lca5 cDNA ORF clone, Danio rerio(zebrafish)

The following lca5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lca5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa48999 XM_009297006.2
Latest version!
Danio rerio Leber congenital amaurosis 5 (lca5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa48999 XM_017353734.1
Latest version!
Danio rerio Leber congenital amaurosis 5 (lca5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa48999 XM_021470010.1
Latest version!
Danio rerio Leber congenital amaurosis 5 (lca5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa48999 XM_021470011.1
Latest version!
Danio rerio Leber congenital amaurosis 5 (lca5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa49001 XM_009297008.2
Latest version!
Danio rerio Leber congenital amaurosis 5 (lca5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa49001 XM_021470014.1
Latest version!
Danio rerio Leber congenital amaurosis 5 (lca5), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa49002 XM_009297009.2
Latest version!
Danio rerio Leber congenital amaurosis 5 (lca5), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa49002 XM_021470015.1
Latest version!
Danio rerio Leber congenital amaurosis 5 (lca5), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa146305 XM_009297006.3
Latest version!
Danio rerio Leber congenital amaurosis 5 (lca5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
ODa146305 XM_017353734.2
Latest version!
Danio rerio Leber congenital amaurosis 5 (lca5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
ODa146306 XM_021473730.1
Latest version!
Danio rerio Leber congenital amaurosis 5 (lca5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
ODa146307 XM_017353735.2
Latest version!
Danio rerio Leber congenital amaurosis 5 (lca5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
ODa146308 XM_009297008.3
Latest version!
Danio rerio Leber congenital amaurosis 5 (lca5), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
ODa146309 XM_009297009.3
Latest version!
Danio rerio Leber congenital amaurosis 5 (lca5), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
ODa146310 XM_017353736.2
Latest version!
Danio rerio Leber congenital amaurosis 5 (lca5), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
ODa49000 XM_021470012.1
Latest version!
Danio rerio Leber congenital amaurosis 5 (lca5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
ODa57513 XM_017353735.1
Latest version!
Danio rerio Leber congenital amaurosis 5 (lca5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
ODa57513 XM_021470013.1
Latest version!
Danio rerio Leber congenital amaurosis 5 (lca5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
ODa57514 XM_021470016.1
Latest version!
Danio rerio Leber congenital amaurosis 5 (lca5), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
ODa57514 XM_017353736.1
Latest version!
Danio rerio Leber congenital amaurosis 5 (lca5), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa48999
    Clone ID Related Accession (Same CDS sequence) XM_009297006.2 , XM_021470011.1 , XM_021470010.1 , XM_017353734.1
    Accession Version XM_009297006.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2304bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product lebercilin isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297006.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGACTTGC ATGAAGATAA TCAGGAATCG GAGCACAGCC GATTGTCTCA CCATAATGAA 
    AGGAAGATCT TAGAATCCCT TCGATCTGGA AAGCAAGAGA ACAATGGAAA AGACATGGGG
    AACTCCGCTG GGGATCATGC ACAGAGCAGA ACTCGAGAGA CAGCTGGAGA TCCAGGCCGC
    AATGGGTTTT CAGATGGAGA GCGCAGCAGT GGCTCGTTCT ACTCTGATGA TTATGAAAAC
    GCCTCCCATT CAGATCGGTC ACTCTCACCG TCAGCTTCTC CATCGCCACC CAGAAGAGGG
    AGATCTAGGA GAGTTTCCAG CAGCCCTTTA CATAAGACAG GTGTGCACAA AGGTGCATCT
    CGCCGTATCC TGTCCCACTC CCATCATCCT CAGCAACGAG CAGGAGCAAT CCGATCCCAA
    AGTTTGAGCA AAGACGCTCC ATCCAAAGAA GTGGACCAGG TCACTAAACG CATGCTGTCA
    GCACGTCTAC TAAAGATCAA CGAGTTGAAG AACGCGTTGT CTGAGCTGAG GCTGCATGCG
    GACGAGCTGC AGCGAGAGAA CCGTTTACTG CGACAGCTGC AGCTCCGGCA AGAGAAAGCT
    TTGCACCGTT ACAATGACAC GGACAGCGAG ATCTCACAGC TGCTGGCTCG TCACAACAAT
    GAAACTCACG TGCTGCGCGA GAGATTGAGA CGCAGTCAAG AGAACCAACG CACAGCTGAG
    CGCAGCCTCA GGGAAAGAGA CGCTCAGCTC CAGCGCTGCC GCAGTCAAAT GCAGAAGCTG
    CAACAACTCG CAGATGACCA GAATCTTGGA GAGAGAGAGG AACTCTCGCG AAAACTAATT
    GGCACTCAAA GCAGATTGCA AGATAGTGAA CACAGAGTGA AGGAATTGGA GAAGAACATG
    GAGCTGAGTA GCGGGAGCTT TCAGAGGCAG CTTGCTAATG AGAGGAGGAA AACTCACGAT
    GCACAACAGG AAGTTAAAGC ACTGCAGGAG GAGGTCGAGA GACTTTGCAC TAAACTAAAG
    GAGAAAGAAA GGGAGCTGGA TGCCCGAAAC ATTTACGCTA ACAGGATGCA CAAAGCTTCT
    TCCAGGAAAG AAGCAGAGAA TGGCAGTAAA CGGAAAGGCT TTAATATAAC CAGCACTAAA
    GCAGTACAGA CAGAGGACAG AACATCCTCA TTGGATTTCC CATCGCCACC TCCAGCTCTT
    ACCAATAATC TTCCACCTGA TGACCAGACT GATGATTACC TTTCTCTGAA GGTTCAAAAG
    CCTACTGTGA ATCACAGGGT AAAAAAGCAG GATCAGCAAA GCAGAGAGCA CAGGACGAGA
    GAATCGAAGG AATGGAAGAT TCAAGAGTTT TGGAGAGAAA GGGAGAAAGA AAGACACATG
    GAATCTGCAA GAGAACAGGA GAAAGAAAAA GAGACACTGC ATGAGAGAGA AAGACAAACA
    GAGCTGCTGA AAGATGTGGA GAGGCAAAGA CTAGATCAAG ATCTCAGCTC AAATGAGAAG
    AACTTTAAAG GAAACAGAGT AGGCCTAAAC AGAGAGGAGG AAGACAGGAG GATTGGCATG
    TCTGTGTCTC AAAAGGAAGA AGAGATCCAA AAGCAGCGTG AACTGGAAGA GGAGCAACAA
    AAAGTGAGAA ATCGGGAGAT CCAGGACTAC AATCAGGAGC GCGTGGAAGA AGAACGTCAG
    CGTAAAGAGC AGCTATTAGC TAAGATGCGT GAGATTGACA TTCAAACTCA AGGTCAGGAC
    TCCGACTTCT TTTCAGATGA CACAGGAAGT GTTCGCTCTC CTCCGCGATT GTCTGAGCAA
    CGGAACCACA ATGGTTTTTT CAAGTTAACA GAGCCAGATG AAAACATAAA CACATTTTCA
    AGCGGAAGAA GAGGAGGCAG TCTCCGAGCT CAGGCACCCA ACGAGGATCT TGATCTTGCT
    TTCGGCAGCT ACGCACCTTC TTTTGGTAAG TCCACCCCAC GCGCAGGTTT GGGGACACGA
    AATGCACAAA ATCACTCCCC TCGAGAACTT GATGAAGGGC TGGATCTGGG TGGCCTGGTC
    AAAGAGAGGA AGTCAAACCT CATGCAGCAG CTTTTTGGGG CCACTGCAAC TCCACCTCCT
    TCAGACCCAT CCAGCAAGAT GGAGATCCTG AGCCCTCCTC TAACAACACA GTTTTCAGGA
    CGCAGGAGGG ACACAGACAC ACCAAATGAA CTAAACATCA GTTCTCTCCC CAACAACAGG
    AGCACTCTAC AAGTCTCAGA GAGCCGACCC GCGGTCAGGG CCATTACATC ATTTGATGAT
    GATATAGAGG AACTCACACT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295281.1
    CDS351..2654
    Translation

    Target ORF information:

    RefSeq Version XM_009297006.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Leber congenital amaurosis 5 (lca5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009297006.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGACTTGC ATGAAGATAA TCAGGAATCG GAGCACAGCC GATTGTCTCA CCATAATGAA 
    AGGAAGATCT TAGAATCCCT TCGATCTGGA AAGCAAGAGA ACAATGGAAA AGACATGGGG
    AACTCCGCTG GGGATCATGC ACAGAGCAGA ACTCGAGAGA CAGCTGGAGA TCCAGGCCGC
    AATGGGTTTT CAGATGGAGA GCGCAGCAGT GGCTCGTTCT ACTCTGATGA TTATGAAAAC
    GCCTCCCATT CAGATCGGTC ACTCTCACCG TCAGCTTCTC CATCGCCACC CAGAAGAGGG
    AGATCTAGGA GAGTTTCCAG CAGCCCTTTA CATAAGACAG GTGTGCACAA AGGTGCATCT
    CGCCGTATCC TGTCCCACTC CCATCATCCT CAGCAACGAG CAGGAGCAAT CCGATCCCAA
    AGTTTGAGCA AAGACGCTCC ATCCAAAGAA GTGGACCAGG TCACTAAACG CATGCTGTCA
    GCACGTCTAC TAAAGATCAA CGAGTTGAAG AACGCGTTGT CTGAGCTGAG GCTGCATGCG
    GACGAGCTGC AGCGAGAGAA CCGTTTACTG CGACAGCTGC AGCTCCGGCA AGAGAAAGCT
    TTGCACCGTT ACAATGACAC GGACAGCGAG ATCTCACAGC TGCTGGCTCG TCACAACAAT
    GAAACTCACG TGCTGCGCGA GAGATTGAGA CGCAGTCAAG AGAACCAACG CACAGCTGAG
    CGCAGCCTCA GGGAAAGAGA CGCTCAGCTC CAGCGCTGCC GCAGTCAAAT GCAGAAGCTG
    CAACAACTCG CAGATGACCA GAATCTTGGA GAGAGAGAGG AACTCTCGCG AAAACTAATT
    GGCACTCAAA GCAGATTGCA AGATAGTGAA CACAGAGTGA AGGAATTGGA GAAGAACATG
    GAGCTGAGTA GCGGGAGCTT TCAGAGGCAG CTTGCTAATG AGAGGAGGAA AACTCACGAT
    GCACAACAGG AAGTTAAAGC ACTGCAGGAG GAGGTCGAGA GACTTTGCAC TAAACTAAAG
    GAGAAAGAAA GGGAGCTGGA TGCCCGAAAC ATTTACGCTA ACAGGATGCA CAAAGCTTCT
    TCCAGGAAAG AAGCAGAGAA TGGCAGTAAA CGGAAAGGCT TTAATATAAC CAGCACTAAA
    GCAGTACAGA CAGAGGACAG AACATCCTCA TTGGATTTCC CATCGCCACC TCCAGCTCTT
    ACCAATAATC TTCCACCTGA TGACCAGACT GATGATTACC TTTCTCTGAA GGTTCAAAAG
    CCTACTGTGA ATCACAGGGT AAAAAAGCAG GATCAGCAAA GCAGAGAGCA CAGGACGAGA
    GAATCGAAGG AATGGAAGAT TCAAGAGTTT TGGAGAGAAA GGGAGAAAGA AAGACACATG
    GAATCTGCAA GAGAACAGGA GAAAGAAAAA GAGACACTGC ATGAGAGAGA AAGACAAACA
    GAGCTGCTGA AAGATGTGGA GAGGCAAAGA CTAGATCAAG ATCTCAGCTC AAATGAGAAG
    AACTTTAAAG GAAACAGAGT AGGCCTAAAC AGAGAGGAGG AAGACAGGAG GATTGGCATG
    TCTGTGTCTC AAAAGGAAGA AGAGATCCAA AAGCAGCGTG AACTGGAAGA GGAGCAACAA
    AAAGTGAGAA ATCGGGAGAT CCAGGACTAC AATCAGGAGC GCGTGGAAGA AGAACGTCAG
    CGTAAAGAGC AGCTATTAGC TAAGATGCGT GAGATTGACA TTCAAACTCA AGGTCAGGAC
    TCCGACTTCT TTTCAGATGA CACAGGAAGT GTTCGCTCTC CTCCGCGATT GTCTGAGCAA
    CGGAACCACA ATGGTTTTTT CAAGTTAACA GAGCCAGATG AAAACATAAA CACATTTTCA
    AGCGGAAGAA GAGGAGGCAG TCTCCGAGCT CAGGCACCCA ACGAGGATCT TGATCTTGCT
    TTCGGCAGCT ACGCACCTTC TTTTGGTAAG TCCACCCCAC GCGCAGGTTT GGGGACACGA
    AATGCACAAA ATCACTCCCC TCGAGAACTT GATGAAGGGC TGGATCTGGG TGGCCTGGTC
    AAAGAGAGGA AGTCAAACCT CATGCAGCAG CTTTTTGGGG CCACTGCAAC TCCACCTCCT
    TCAGACCCAT CCAGCAAGAT GGAGATCCTG AGCCCTCCTC TAACAACACA GTTTTCAGGA
    CGCAGGAGGG ACACAGACAC ACCAAATGAA CTAAACATCA GTTCTCTCCC CAACAACAGG
    AGCACTCTAC AAGTCTCAGA GAGCCGACCC GCGGTCAGGG CCATTACATC ATTTGATGAT
    GATATAGAGG AACTCACACT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48999
    Clone ID Related Accession (Same CDS sequence) XM_009297006.2 , XM_021470011.1 , XM_021470010.1 , XM_017353734.1
    Accession Version XM_021470011.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2304bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product lebercilin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGACTTGC ATGAAGATAA TCAGGAATCG GAGCACAGCC GATTGTCTCA CCATAATGAA 
    AGGAAGATCT TAGAATCCCT TCGATCTGGA AAGCAAGAGA ACAATGGAAA AGACATGGGG
    AACTCCGCTG GGGATCATGC ACAGAGCAGA ACTCGAGAGA CAGCTGGAGA TCCAGGCCGC
    AATGGGTTTT CAGATGGAGA GCGCAGCAGT GGCTCGTTCT ACTCTGATGA TTATGAAAAC
    GCCTCCCATT CAGATCGGTC ACTCTCACCG TCAGCTTCTC CATCGCCACC CAGAAGAGGG
    AGATCTAGGA GAGTTTCCAG CAGCCCTTTA CATAAGACAG GTGTGCACAA AGGTGCATCT
    CGCCGTATCC TGTCCCACTC CCATCATCCT CAGCAACGAG CAGGAGCAAT CCGATCCCAA
    AGTTTGAGCA AAGACGCTCC ATCCAAAGAA GTGGACCAGG TCACTAAACG CATGCTGTCA
    GCACGTCTAC TAAAGATCAA CGAGTTGAAG AACGCGTTGT CTGAGCTGAG GCTGCATGCG
    GACGAGCTGC AGCGAGAGAA CCGTTTACTG CGACAGCTGC AGCTCCGGCA AGAGAAAGCT
    TTGCACCGTT ACAATGACAC GGACAGCGAG ATCTCACAGC TGCTGGCTCG TCACAACAAT
    GAAACTCACG TGCTGCGCGA GAGATTGAGA CGCAGTCAAG AGAACCAACG CACAGCTGAG
    CGCAGCCTCA GGGAAAGAGA CGCTCAGCTC CAGCGCTGCC GCAGTCAAAT GCAGAAGCTG
    CAACAACTCG CAGATGACCA GAATCTTGGA GAGAGAGAGG AACTCTCGCG AAAACTAATT
    GGCACTCAAA GCAGATTGCA AGATAGTGAA CACAGAGTGA AGGAATTGGA GAAGAACATG
    GAGCTGAGTA GCGGGAGCTT TCAGAGGCAG CTTGCTAATG AGAGGAGGAA AACTCACGAT
    GCACAACAGG AAGTTAAAGC ACTGCAGGAG GAGGTCGAGA GACTTTGCAC TAAACTAAAG
    GAGAAAGAAA GGGAGCTGGA TGCCCGAAAC ATTTACGCTA ACAGGATGCA CAAAGCTTCT
    TCCAGGAAAG AAGCAGAGAA TGGCAGTAAA CGGAAAGGCT TTAATATAAC CAGCACTAAA
    GCAGTACAGA CAGAGGACAG AACATCCTCA TTGGATTTCC CATCGCCACC TCCAGCTCTT
    ACCAATAATC TTCCACCTGA TGACCAGACT GATGATTACC TTTCTCTGAA GGTTCAAAAG
    CCTACTGTGA ATCACAGGGT AAAAAAGCAG GATCAGCAAA GCAGAGAGCA CAGGACGAGA
    GAATCGAAGG AATGGAAGAT TCAAGAGTTT TGGAGAGAAA GGGAGAAAGA AAGACACATG
    GAATCTGCAA GAGAACAGGA GAAAGAAAAA GAGACACTGC ATGAGAGAGA AAGACAAACA
    GAGCTGCTGA AAGATGTGGA GAGGCAAAGA CTAGATCAAG ATCTCAGCTC AAATGAGAAG
    AACTTTAAAG GAAACAGAGT AGGCCTAAAC AGAGAGGAGG AAGACAGGAG GATTGGCATG
    TCTGTGTCTC AAAAGGAAGA AGAGATCCAA AAGCAGCGTG AACTGGAAGA GGAGCAACAA
    AAAGTGAGAA ATCGGGAGAT CCAGGACTAC AATCAGGAGC GCGTGGAAGA AGAACGTCAG
    CGTAAAGAGC AGCTATTAGC TAAGATGCGT GAGATTGACA TTCAAACTCA AGGTCAGGAC
    TCCGACTTCT TTTCAGATGA CACAGGAAGT GTTCGCTCTC CTCCGCGATT GTCTGAGCAA
    CGGAACCACA ATGGTTTTTT CAAGTTAACA GAGCCAGATG AAAACATAAA CACATTTTCA
    AGCGGAAGAA GAGGAGGCAG TCTCCGAGCT CAGGCACCCA ACGAGGATCT TGATCTTGCT
    TTCGGCAGCT ACGCACCTTC TTTTGGTAAG TCCACCCCAC GCGCAGGTTT GGGGACACGA
    AATGCACAAA ATCACTCCCC TCGAGAACTT GATGAAGGGC TGGATCTGGG TGGCCTGGTC
    AAAGAGAGGA AGTCAAACCT CATGCAGCAG CTTTTTGGGG CCACTGCAAC TCCACCTCCT
    TCAGACCCAT CCAGCAAGAT GGAGATCCTG AGCCCTCCTC TAACAACACA GTTTTCAGGA
    CGCAGGAGGG ACACAGACAC ACCAAATGAA CTAAACATCA GTTCTCTCCC CAACAACAGG
    AGCACTCTAC AAGTCTCAGA GAGCCGACCC GCGGTCAGGG CCATTACATC ATTTGATGAT
    GATATAGAGG AACTCACACT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021325686.1
    CDS396..2699
    Translation

    Target ORF information:

    RefSeq Version XM_021470011.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Leber congenital amaurosis 5 (lca5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021470011.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGACTTGC ATGAAGATAA TCAGGAATCG GAGCACAGCC GATTGTCTCA CCATAATGAA 
    AGGAAGATCT TAGAATCCCT TCGATCTGGA AAGCAAGAGA ACAATGGAAA AGACATGGGG
    AACTCCGCTG GGGATCATGC ACAGAGCAGA ACTCGAGAGA CAGCTGGAGA TCCAGGCCGC
    AATGGGTTTT CAGATGGAGA GCGCAGCAGT GGCTCGTTCT ACTCTGATGA TTATGAAAAC
    GCCTCCCATT CAGATCGGTC ACTCTCACCG TCAGCTTCTC CATCGCCACC CAGAAGAGGG
    AGATCTAGGA GAGTTTCCAG CAGCCCTTTA CATAAGACAG GTGTGCACAA AGGTGCATCT
    CGCCGTATCC TGTCCCACTC CCATCATCCT CAGCAACGAG CAGGAGCAAT CCGATCCCAA
    AGTTTGAGCA AAGACGCTCC ATCCAAAGAA GTGGACCAGG TCACTAAACG CATGCTGTCA
    GCACGTCTAC TAAAGATCAA CGAGTTGAAG AACGCGTTGT CTGAGCTGAG GCTGCATGCG
    GACGAGCTGC AGCGAGAGAA CCGTTTACTG CGACAGCTGC AGCTCCGGCA AGAGAAAGCT
    TTGCACCGTT ACAATGACAC GGACAGCGAG ATCTCACAGC TGCTGGCTCG TCACAACAAT
    GAAACTCACG TGCTGCGCGA GAGATTGAGA CGCAGTCAAG AGAACCAACG CACAGCTGAG
    CGCAGCCTCA GGGAAAGAGA CGCTCAGCTC CAGCGCTGCC GCAGTCAAAT GCAGAAGCTG
    CAACAACTCG CAGATGACCA GAATCTTGGA GAGAGAGAGG AACTCTCGCG AAAACTAATT
    GGCACTCAAA GCAGATTGCA AGATAGTGAA CACAGAGTGA AGGAATTGGA GAAGAACATG
    GAGCTGAGTA GCGGGAGCTT TCAGAGGCAG CTTGCTAATG AGAGGAGGAA AACTCACGAT
    GCACAACAGG AAGTTAAAGC ACTGCAGGAG GAGGTCGAGA GACTTTGCAC TAAACTAAAG
    GAGAAAGAAA GGGAGCTGGA TGCCCGAAAC ATTTACGCTA ACAGGATGCA CAAAGCTTCT
    TCCAGGAAAG AAGCAGAGAA TGGCAGTAAA CGGAAAGGCT TTAATATAAC CAGCACTAAA
    GCAGTACAGA CAGAGGACAG AACATCCTCA TTGGATTTCC CATCGCCACC TCCAGCTCTT
    ACCAATAATC TTCCACCTGA TGACCAGACT GATGATTACC TTTCTCTGAA GGTTCAAAAG
    CCTACTGTGA ATCACAGGGT AAAAAAGCAG GATCAGCAAA GCAGAGAGCA CAGGACGAGA
    GAATCGAAGG AATGGAAGAT TCAAGAGTTT TGGAGAGAAA GGGAGAAAGA AAGACACATG
    GAATCTGCAA GAGAACAGGA GAAAGAAAAA GAGACACTGC ATGAGAGAGA AAGACAAACA
    GAGCTGCTGA AAGATGTGGA GAGGCAAAGA CTAGATCAAG ATCTCAGCTC AAATGAGAAG
    AACTTTAAAG GAAACAGAGT AGGCCTAAAC AGAGAGGAGG AAGACAGGAG GATTGGCATG
    TCTGTGTCTC AAAAGGAAGA AGAGATCCAA AAGCAGCGTG AACTGGAAGA GGAGCAACAA
    AAAGTGAGAA ATCGGGAGAT CCAGGACTAC AATCAGGAGC GCGTGGAAGA AGAACGTCAG
    CGTAAAGAGC AGCTATTAGC TAAGATGCGT GAGATTGACA TTCAAACTCA AGGTCAGGAC
    TCCGACTTCT TTTCAGATGA CACAGGAAGT GTTCGCTCTC CTCCGCGATT GTCTGAGCAA
    CGGAACCACA ATGGTTTTTT CAAGTTAACA GAGCCAGATG AAAACATAAA CACATTTTCA
    AGCGGAAGAA GAGGAGGCAG TCTCCGAGCT CAGGCACCCA ACGAGGATCT TGATCTTGCT
    TTCGGCAGCT ACGCACCTTC TTTTGGTAAG TCCACCCCAC GCGCAGGTTT GGGGACACGA
    AATGCACAAA ATCACTCCCC TCGAGAACTT GATGAAGGGC TGGATCTGGG TGGCCTGGTC
    AAAGAGAGGA AGTCAAACCT CATGCAGCAG CTTTTTGGGG CCACTGCAAC TCCACCTCCT
    TCAGACCCAT CCAGCAAGAT GGAGATCCTG AGCCCTCCTC TAACAACACA GTTTTCAGGA
    CGCAGGAGGG ACACAGACAC ACCAAATGAA CTAAACATCA GTTCTCTCCC CAACAACAGG
    AGCACTCTAC AAGTCTCAGA GAGCCGACCC GCGGTCAGGG CCATTACATC ATTTGATGAT
    GATATAGAGG AACTCACACT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48999
    Clone ID Related Accession (Same CDS sequence) XM_009297006.2 , XM_021470011.1 , XM_021470010.1 , XM_017353734.1
    Accession Version XM_021470010.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2304bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product lebercilin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGACTTGC ATGAAGATAA TCAGGAATCG GAGCACAGCC GATTGTCTCA CCATAATGAA 
    AGGAAGATCT TAGAATCCCT TCGATCTGGA AAGCAAGAGA ACAATGGAAA AGACATGGGG
    AACTCCGCTG GGGATCATGC ACAGAGCAGA ACTCGAGAGA CAGCTGGAGA TCCAGGCCGC
    AATGGGTTTT CAGATGGAGA GCGCAGCAGT GGCTCGTTCT ACTCTGATGA TTATGAAAAC
    GCCTCCCATT CAGATCGGTC ACTCTCACCG TCAGCTTCTC CATCGCCACC CAGAAGAGGG
    AGATCTAGGA GAGTTTCCAG CAGCCCTTTA CATAAGACAG GTGTGCACAA AGGTGCATCT
    CGCCGTATCC TGTCCCACTC CCATCATCCT CAGCAACGAG CAGGAGCAAT CCGATCCCAA
    AGTTTGAGCA AAGACGCTCC ATCCAAAGAA GTGGACCAGG TCACTAAACG CATGCTGTCA
    GCACGTCTAC TAAAGATCAA CGAGTTGAAG AACGCGTTGT CTGAGCTGAG GCTGCATGCG
    GACGAGCTGC AGCGAGAGAA CCGTTTACTG CGACAGCTGC AGCTCCGGCA AGAGAAAGCT
    TTGCACCGTT ACAATGACAC GGACAGCGAG ATCTCACAGC TGCTGGCTCG TCACAACAAT
    GAAACTCACG TGCTGCGCGA GAGATTGAGA CGCAGTCAAG AGAACCAACG CACAGCTGAG
    CGCAGCCTCA GGGAAAGAGA CGCTCAGCTC CAGCGCTGCC GCAGTCAAAT GCAGAAGCTG
    CAACAACTCG CAGATGACCA GAATCTTGGA GAGAGAGAGG AACTCTCGCG AAAACTAATT
    GGCACTCAAA GCAGATTGCA AGATAGTGAA CACAGAGTGA AGGAATTGGA GAAGAACATG
    GAGCTGAGTA GCGGGAGCTT TCAGAGGCAG CTTGCTAATG AGAGGAGGAA AACTCACGAT
    GCACAACAGG AAGTTAAAGC ACTGCAGGAG GAGGTCGAGA GACTTTGCAC TAAACTAAAG
    GAGAAAGAAA GGGAGCTGGA TGCCCGAAAC ATTTACGCTA ACAGGATGCA CAAAGCTTCT
    TCCAGGAAAG AAGCAGAGAA TGGCAGTAAA CGGAAAGGCT TTAATATAAC CAGCACTAAA
    GCAGTACAGA CAGAGGACAG AACATCCTCA TTGGATTTCC CATCGCCACC TCCAGCTCTT
    ACCAATAATC TTCCACCTGA TGACCAGACT GATGATTACC TTTCTCTGAA GGTTCAAAAG
    CCTACTGTGA ATCACAGGGT AAAAAAGCAG GATCAGCAAA GCAGAGAGCA CAGGACGAGA
    GAATCGAAGG AATGGAAGAT TCAAGAGTTT TGGAGAGAAA GGGAGAAAGA AAGACACATG
    GAATCTGCAA GAGAACAGGA GAAAGAAAAA GAGACACTGC ATGAGAGAGA AAGACAAACA
    GAGCTGCTGA AAGATGTGGA GAGGCAAAGA CTAGATCAAG ATCTCAGCTC AAATGAGAAG
    AACTTTAAAG GAAACAGAGT AGGCCTAAAC AGAGAGGAGG AAGACAGGAG GATTGGCATG
    TCTGTGTCTC AAAAGGAAGA AGAGATCCAA AAGCAGCGTG AACTGGAAGA GGAGCAACAA
    AAAGTGAGAA ATCGGGAGAT CCAGGACTAC AATCAGGAGC GCGTGGAAGA AGAACGTCAG
    CGTAAAGAGC AGCTATTAGC TAAGATGCGT GAGATTGACA TTCAAACTCA AGGTCAGGAC
    TCCGACTTCT TTTCAGATGA CACAGGAAGT GTTCGCTCTC CTCCGCGATT GTCTGAGCAA
    CGGAACCACA ATGGTTTTTT CAAGTTAACA GAGCCAGATG AAAACATAAA CACATTTTCA
    AGCGGAAGAA GAGGAGGCAG TCTCCGAGCT CAGGCACCCA ACGAGGATCT TGATCTTGCT
    TTCGGCAGCT ACGCACCTTC TTTTGGTAAG TCCACCCCAC GCGCAGGTTT GGGGACACGA
    AATGCACAAA ATCACTCCCC TCGAGAACTT GATGAAGGGC TGGATCTGGG TGGCCTGGTC
    AAAGAGAGGA AGTCAAACCT CATGCAGCAG CTTTTTGGGG CCACTGCAAC TCCACCTCCT
    TCAGACCCAT CCAGCAAGAT GGAGATCCTG AGCCCTCCTC TAACAACACA GTTTTCAGGA
    CGCAGGAGGG ACACAGACAC ACCAAATGAA CTAAACATCA GTTCTCTCCC CAACAACAGG
    AGCACTCTAC AAGTCTCAGA GAGCCGACCC GCGGTCAGGG CCATTACATC ATTTGATGAT
    GATATAGAGG AACTCACACT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021325685.1
    CDS399..2702
    Translation

    Target ORF information:

    RefSeq Version XM_021470010.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Leber congenital amaurosis 5 (lca5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021470010.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGACTTGC ATGAAGATAA TCAGGAATCG GAGCACAGCC GATTGTCTCA CCATAATGAA 
    AGGAAGATCT TAGAATCCCT TCGATCTGGA AAGCAAGAGA ACAATGGAAA AGACATGGGG
    AACTCCGCTG GGGATCATGC ACAGAGCAGA ACTCGAGAGA CAGCTGGAGA TCCAGGCCGC
    AATGGGTTTT CAGATGGAGA GCGCAGCAGT GGCTCGTTCT ACTCTGATGA TTATGAAAAC
    GCCTCCCATT CAGATCGGTC ACTCTCACCG TCAGCTTCTC CATCGCCACC CAGAAGAGGG
    AGATCTAGGA GAGTTTCCAG CAGCCCTTTA CATAAGACAG GTGTGCACAA AGGTGCATCT
    CGCCGTATCC TGTCCCACTC CCATCATCCT CAGCAACGAG CAGGAGCAAT CCGATCCCAA
    AGTTTGAGCA AAGACGCTCC ATCCAAAGAA GTGGACCAGG TCACTAAACG CATGCTGTCA
    GCACGTCTAC TAAAGATCAA CGAGTTGAAG AACGCGTTGT CTGAGCTGAG GCTGCATGCG
    GACGAGCTGC AGCGAGAGAA CCGTTTACTG CGACAGCTGC AGCTCCGGCA AGAGAAAGCT
    TTGCACCGTT ACAATGACAC GGACAGCGAG ATCTCACAGC TGCTGGCTCG TCACAACAAT
    GAAACTCACG TGCTGCGCGA GAGATTGAGA CGCAGTCAAG AGAACCAACG CACAGCTGAG
    CGCAGCCTCA GGGAAAGAGA CGCTCAGCTC CAGCGCTGCC GCAGTCAAAT GCAGAAGCTG
    CAACAACTCG CAGATGACCA GAATCTTGGA GAGAGAGAGG AACTCTCGCG AAAACTAATT
    GGCACTCAAA GCAGATTGCA AGATAGTGAA CACAGAGTGA AGGAATTGGA GAAGAACATG
    GAGCTGAGTA GCGGGAGCTT TCAGAGGCAG CTTGCTAATG AGAGGAGGAA AACTCACGAT
    GCACAACAGG AAGTTAAAGC ACTGCAGGAG GAGGTCGAGA GACTTTGCAC TAAACTAAAG
    GAGAAAGAAA GGGAGCTGGA TGCCCGAAAC ATTTACGCTA ACAGGATGCA CAAAGCTTCT
    TCCAGGAAAG AAGCAGAGAA TGGCAGTAAA CGGAAAGGCT TTAATATAAC CAGCACTAAA
    GCAGTACAGA CAGAGGACAG AACATCCTCA TTGGATTTCC CATCGCCACC TCCAGCTCTT
    ACCAATAATC TTCCACCTGA TGACCAGACT GATGATTACC TTTCTCTGAA GGTTCAAAAG
    CCTACTGTGA ATCACAGGGT AAAAAAGCAG GATCAGCAAA GCAGAGAGCA CAGGACGAGA
    GAATCGAAGG AATGGAAGAT TCAAGAGTTT TGGAGAGAAA GGGAGAAAGA AAGACACATG
    GAATCTGCAA GAGAACAGGA GAAAGAAAAA GAGACACTGC ATGAGAGAGA AAGACAAACA
    GAGCTGCTGA AAGATGTGGA GAGGCAAAGA CTAGATCAAG ATCTCAGCTC AAATGAGAAG
    AACTTTAAAG GAAACAGAGT AGGCCTAAAC AGAGAGGAGG AAGACAGGAG GATTGGCATG
    TCTGTGTCTC AAAAGGAAGA AGAGATCCAA AAGCAGCGTG AACTGGAAGA GGAGCAACAA
    AAAGTGAGAA ATCGGGAGAT CCAGGACTAC AATCAGGAGC GCGTGGAAGA AGAACGTCAG
    CGTAAAGAGC AGCTATTAGC TAAGATGCGT GAGATTGACA TTCAAACTCA AGGTCAGGAC
    TCCGACTTCT TTTCAGATGA CACAGGAAGT GTTCGCTCTC CTCCGCGATT GTCTGAGCAA
    CGGAACCACA ATGGTTTTTT CAAGTTAACA GAGCCAGATG AAAACATAAA CACATTTTCA
    AGCGGAAGAA GAGGAGGCAG TCTCCGAGCT CAGGCACCCA ACGAGGATCT TGATCTTGCT
    TTCGGCAGCT ACGCACCTTC TTTTGGTAAG TCCACCCCAC GCGCAGGTTT GGGGACACGA
    AATGCACAAA ATCACTCCCC TCGAGAACTT GATGAAGGGC TGGATCTGGG TGGCCTGGTC
    AAAGAGAGGA AGTCAAACCT CATGCAGCAG CTTTTTGGGG CCACTGCAAC TCCACCTCCT
    TCAGACCCAT CCAGCAAGAT GGAGATCCTG AGCCCTCCTC TAACAACACA GTTTTCAGGA
    CGCAGGAGGG ACACAGACAC ACCAAATGAA CTAAACATCA GTTCTCTCCC CAACAACAGG
    AGCACTCTAC AAGTCTCAGA GAGCCGACCC GCGGTCAGGG CCATTACATC ATTTGATGAT
    GATATAGAGG AACTCACACT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48999
    Clone ID Related Accession (Same CDS sequence) XM_009297006.2 , XM_021470011.1 , XM_021470010.1 , XM_017353734.1
    Accession Version XM_017353734.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2304bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product lebercilin isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGACTTGC ATGAAGATAA TCAGGAATCG GAGCACAGCC GATTGTCTCA CCATAATGAA 
    AGGAAGATCT TAGAATCCCT TCGATCTGGA AAGCAAGAGA ACAATGGAAA AGACATGGGG
    AACTCCGCTG GGGATCATGC ACAGAGCAGA ACTCGAGAGA CAGCTGGAGA TCCAGGCCGC
    AATGGGTTTT CAGATGGAGA GCGCAGCAGT GGCTCGTTCT ACTCTGATGA TTATGAAAAC
    GCCTCCCATT CAGATCGGTC ACTCTCACCG TCAGCTTCTC CATCGCCACC CAGAAGAGGG
    AGATCTAGGA GAGTTTCCAG CAGCCCTTTA CATAAGACAG GTGTGCACAA AGGTGCATCT
    CGCCGTATCC TGTCCCACTC CCATCATCCT CAGCAACGAG CAGGAGCAAT CCGATCCCAA
    AGTTTGAGCA AAGACGCTCC ATCCAAAGAA GTGGACCAGG TCACTAAACG CATGCTGTCA
    GCACGTCTAC TAAAGATCAA CGAGTTGAAG AACGCGTTGT CTGAGCTGAG GCTGCATGCG
    GACGAGCTGC AGCGAGAGAA CCGTTTACTG CGACAGCTGC AGCTCCGGCA AGAGAAAGCT
    TTGCACCGTT ACAATGACAC GGACAGCGAG ATCTCACAGC TGCTGGCTCG TCACAACAAT
    GAAACTCACG TGCTGCGCGA GAGATTGAGA CGCAGTCAAG AGAACCAACG CACAGCTGAG
    CGCAGCCTCA GGGAAAGAGA CGCTCAGCTC CAGCGCTGCC GCAGTCAAAT GCAGAAGCTG
    CAACAACTCG CAGATGACCA GAATCTTGGA GAGAGAGAGG AACTCTCGCG AAAACTAATT
    GGCACTCAAA GCAGATTGCA AGATAGTGAA CACAGAGTGA AGGAATTGGA GAAGAACATG
    GAGCTGAGTA GCGGGAGCTT TCAGAGGCAG CTTGCTAATG AGAGGAGGAA AACTCACGAT
    GCACAACAGG AAGTTAAAGC ACTGCAGGAG GAGGTCGAGA GACTTTGCAC TAAACTAAAG
    GAGAAAGAAA GGGAGCTGGA TGCCCGAAAC ATTTACGCTA ACAGGATGCA CAAAGCTTCT
    TCCAGGAAAG AAGCAGAGAA TGGCAGTAAA CGGAAAGGCT TTAATATAAC CAGCACTAAA
    GCAGTACAGA CAGAGGACAG AACATCCTCA TTGGATTTCC CATCGCCACC TCCAGCTCTT
    ACCAATAATC TTCCACCTGA TGACCAGACT GATGATTACC TTTCTCTGAA GGTTCAAAAG
    CCTACTGTGA ATCACAGGGT AAAAAAGCAG GATCAGCAAA GCAGAGAGCA CAGGACGAGA
    GAATCGAAGG AATGGAAGAT TCAAGAGTTT TGGAGAGAAA GGGAGAAAGA AAGACACATG
    GAATCTGCAA GAGAACAGGA GAAAGAAAAA GAGACACTGC ATGAGAGAGA AAGACAAACA
    GAGCTGCTGA AAGATGTGGA GAGGCAAAGA CTAGATCAAG ATCTCAGCTC AAATGAGAAG
    AACTTTAAAG GAAACAGAGT AGGCCTAAAC AGAGAGGAGG AAGACAGGAG GATTGGCATG
    TCTGTGTCTC AAAAGGAAGA AGAGATCCAA AAGCAGCGTG AACTGGAAGA GGAGCAACAA
    AAAGTGAGAA ATCGGGAGAT CCAGGACTAC AATCAGGAGC GCGTGGAAGA AGAACGTCAG
    CGTAAAGAGC AGCTATTAGC TAAGATGCGT GAGATTGACA TTCAAACTCA AGGTCAGGAC
    TCCGACTTCT TTTCAGATGA CACAGGAAGT GTTCGCTCTC CTCCGCGATT GTCTGAGCAA
    CGGAACCACA ATGGTTTTTT CAAGTTAACA GAGCCAGATG AAAACATAAA CACATTTTCA
    AGCGGAAGAA GAGGAGGCAG TCTCCGAGCT CAGGCACCCA ACGAGGATCT TGATCTTGCT
    TTCGGCAGCT ACGCACCTTC TTTTGGTAAG TCCACCCCAC GCGCAGGTTT GGGGACACGA
    AATGCACAAA ATCACTCCCC TCGAGAACTT GATGAAGGGC TGGATCTGGG TGGCCTGGTC
    AAAGAGAGGA AGTCAAACCT CATGCAGCAG CTTTTTGGGG CCACTGCAAC TCCACCTCCT
    TCAGACCCAT CCAGCAAGAT GGAGATCCTG AGCCCTCCTC TAACAACACA GTTTTCAGGA
    CGCAGGAGGG ACACAGACAC ACCAAATGAA CTAAACATCA GTTCTCTCCC CAACAACAGG
    AGCACTCTAC AAGTCTCAGA GAGCCGACCC GCGGTCAGGG CCATTACATC ATTTGATGAT
    GATATAGAGG AACTCACACT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017209223.1
    CDS348..2651
    Translation

    Target ORF information:

    RefSeq Version XM_017353734.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Leber congenital amaurosis 5 (lca5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017353734.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGACTTGC ATGAAGATAA TCAGGAATCG GAGCACAGCC GATTGTCTCA CCATAATGAA 
    AGGAAGATCT TAGAATCCCT TCGATCTGGA AAGCAAGAGA ACAATGGAAA AGACATGGGG
    AACTCCGCTG GGGATCATGC ACAGAGCAGA ACTCGAGAGA CAGCTGGAGA TCCAGGCCGC
    AATGGGTTTT CAGATGGAGA GCGCAGCAGT GGCTCGTTCT ACTCTGATGA TTATGAAAAC
    GCCTCCCATT CAGATCGGTC ACTCTCACCG TCAGCTTCTC CATCGCCACC CAGAAGAGGG
    AGATCTAGGA GAGTTTCCAG CAGCCCTTTA CATAAGACAG GTGTGCACAA AGGTGCATCT
    CGCCGTATCC TGTCCCACTC CCATCATCCT CAGCAACGAG CAGGAGCAAT CCGATCCCAA
    AGTTTGAGCA AAGACGCTCC ATCCAAAGAA GTGGACCAGG TCACTAAACG CATGCTGTCA
    GCACGTCTAC TAAAGATCAA CGAGTTGAAG AACGCGTTGT CTGAGCTGAG GCTGCATGCG
    GACGAGCTGC AGCGAGAGAA CCGTTTACTG CGACAGCTGC AGCTCCGGCA AGAGAAAGCT
    TTGCACCGTT ACAATGACAC GGACAGCGAG ATCTCACAGC TGCTGGCTCG TCACAACAAT
    GAAACTCACG TGCTGCGCGA GAGATTGAGA CGCAGTCAAG AGAACCAACG CACAGCTGAG
    CGCAGCCTCA GGGAAAGAGA CGCTCAGCTC CAGCGCTGCC GCAGTCAAAT GCAGAAGCTG
    CAACAACTCG CAGATGACCA GAATCTTGGA GAGAGAGAGG AACTCTCGCG AAAACTAATT
    GGCACTCAAA GCAGATTGCA AGATAGTGAA CACAGAGTGA AGGAATTGGA GAAGAACATG
    GAGCTGAGTA GCGGGAGCTT TCAGAGGCAG CTTGCTAATG AGAGGAGGAA AACTCACGAT
    GCACAACAGG AAGTTAAAGC ACTGCAGGAG GAGGTCGAGA GACTTTGCAC TAAACTAAAG
    GAGAAAGAAA GGGAGCTGGA TGCCCGAAAC ATTTACGCTA ACAGGATGCA CAAAGCTTCT
    TCCAGGAAAG AAGCAGAGAA TGGCAGTAAA CGGAAAGGCT TTAATATAAC CAGCACTAAA
    GCAGTACAGA CAGAGGACAG AACATCCTCA TTGGATTTCC CATCGCCACC TCCAGCTCTT
    ACCAATAATC TTCCACCTGA TGACCAGACT GATGATTACC TTTCTCTGAA GGTTCAAAAG
    CCTACTGTGA ATCACAGGGT AAAAAAGCAG GATCAGCAAA GCAGAGAGCA CAGGACGAGA
    GAATCGAAGG AATGGAAGAT TCAAGAGTTT TGGAGAGAAA GGGAGAAAGA AAGACACATG
    GAATCTGCAA GAGAACAGGA GAAAGAAAAA GAGACACTGC ATGAGAGAGA AAGACAAACA
    GAGCTGCTGA AAGATGTGGA GAGGCAAAGA CTAGATCAAG ATCTCAGCTC AAATGAGAAG
    AACTTTAAAG GAAACAGAGT AGGCCTAAAC AGAGAGGAGG AAGACAGGAG GATTGGCATG
    TCTGTGTCTC AAAAGGAAGA AGAGATCCAA AAGCAGCGTG AACTGGAAGA GGAGCAACAA
    AAAGTGAGAA ATCGGGAGAT CCAGGACTAC AATCAGGAGC GCGTGGAAGA AGAACGTCAG
    CGTAAAGAGC AGCTATTAGC TAAGATGCGT GAGATTGACA TTCAAACTCA AGGTCAGGAC
    TCCGACTTCT TTTCAGATGA CACAGGAAGT GTTCGCTCTC CTCCGCGATT GTCTGAGCAA
    CGGAACCACA ATGGTTTTTT CAAGTTAACA GAGCCAGATG AAAACATAAA CACATTTTCA
    AGCGGAAGAA GAGGAGGCAG TCTCCGAGCT CAGGCACCCA ACGAGGATCT TGATCTTGCT
    TTCGGCAGCT ACGCACCTTC TTTTGGTAAG TCCACCCCAC GCGCAGGTTT GGGGACACGA
    AATGCACAAA ATCACTCCCC TCGAGAACTT GATGAAGGGC TGGATCTGGG TGGCCTGGTC
    AAAGAGAGGA AGTCAAACCT CATGCAGCAG CTTTTTGGGG CCACTGCAAC TCCACCTCCT
    TCAGACCCAT CCAGCAAGAT GGAGATCCTG AGCCCTCCTC TAACAACACA GTTTTCAGGA
    CGCAGGAGGG ACACAGACAC ACCAAATGAA CTAAACATCA GTTCTCTCCC CAACAACAGG
    AGCACTCTAC AAGTCTCAGA GAGCCGACCC GCGGTCAGGG CCATTACATC ATTTGATGAT
    GATATAGAGG AACTCACACT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa49001
    Clone ID Related Accession (Same CDS sequence) XM_021470014.1 , XM_009297008.2
    Accession Version XM_021470014.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2268bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product lebercilin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGACTTGC ATGAAGATAA TCAGGAATCG GAGCACAGCC GATTGTCTCA CCATAATGAA 
    AGGAAGATCT TAGAATCCCT TCGATCTGGA AAGCAAGAGA ACAATGGAAA AGACATGGGG
    AACTCCGCTG GGGATCATGC ACAGAGCAGA ACTCGAGAGA CAGCTGGAGA TCCAGGCCGC
    AATGGGTTTT CAGATGGAGA GCGCAGCAGT GGCTCGTTCT ACTCTGATGA TTATGAAAAC
    GCCTCCCATT CAGATCGGTC ACTCTCACCG TCAGCTTCTC CATCGCCACC CAGAAGAGGG
    AGATCTAGGA GAGTTTCCAG CAGCCCTTTA CATAAGACAG GTGTGCACAA AGGTGCATCT
    CGCCGTATCC TGTCCCACTC CCATCATCCT CAGCAACGAG CAGGAGCAAT CCGATCCCAA
    AGTTTGAGCA AAGACGCTCC ATCCAAAGAA GTGGACCAGG TCACTAAACG CATGCTGTCA
    GCACGTCTAC TAAAGATCAA CGAGTTGAAG AACGCGTTGT CTGAGCTGAG GCTGCATGCG
    GACGAGCTGC AGCGAGAGAA CCGTTTACTG CGACAGCTGC AGCTCCGGCA AGAGAAAGCT
    TTGCACCGTT ACAATGACAC GGACAGCGAG ATCTCACAGC TGCTGGCTCG TCACAACAAT
    GAAACTCACG TGCTGCGCGA GAGATTGAGA CGCAGTCAAG AGAACCAACG CACAGCTGAG
    CGCAGCCTCA GGGAAAGAGA CGCTCAGCTC CAGCGCTGCC GCAGTCAAAT GCAGAAGCTG
    CAACAACTCG CAGATGACCA GAATCTTGGA GAGAGAGAGG AACTCTCGCG AAAACTAATT
    GGCACTCAAA GCAGATTGCA AGATAGTGAA CACAGAGTGA AGGAATTGGA GAAGAACATG
    GAGCTGAGTA GCGGGAGCTT TCAGAGGCAG CTTGCTAATG AGAGGAGGAA AACTCACGAT
    GCACAACAGG AAGTTAAAGC ACTGCAGGAG GAGGTCGAGA GACTTTGCAC TAAACTAAAG
    GAGAAAGAAA GGGAGCTGGA TGCCCGAAAC ATTTACGCTA ACAGGATGCA CAAAGCTTCT
    TCCAGGAAAG AAGCAGAGAA TGGCAGTAAA CGGAAAGGCT TTAATATAAC CAGCACTAAA
    GCAGTACAGA CAGAGGACAG AACATCCTCA TTGGATTTCC CATCGCCACC TCCAGCTCTT
    ACCAATAATC TTCCACCTGA TGACCAGACT GATGATTACC TTTCTCTGAA GCAGGATCAG
    CAAAGCAGAG AGCACAGGAC GAGAGAATCG AAGGAATGGA AGATTCAAGA GTTTTGGAGA
    GAAAGGGAGA AAGAAAGACA CATGGAATCT GCAAGAGAAC AGGAGAAAGA AAAAGAGACA
    CTGCATGAGA GAGAAAGACA AACAGAGCTG CTGAAAGATG TGGAGAGGCA AAGACTAGAT
    CAAGATCTCA GCTCAAATGA GAAGAACTTT AAAGGAAACA GAGTAGGCCT AAACAGAGAG
    GAGGAAGACA GGAGGATTGG CATGTCTGTG TCTCAAAAGG AAGAAGAGAT CCAAAAGCAG
    CGTGAACTGG AAGAGGAGCA ACAAAAAGTG AGAAATCGGG AGATCCAGGA CTACAATCAG
    GAGCGCGTGG AAGAAGAACG TCAGCGTAAA GAGCAGCTAT TAGCTAAGAT GCGTGAGATT
    GACATTCAAA CTCAAGGTCA GGACTCCGAC TTCTTTTCAG ATGACACAGG AAGTGTTCGC
    TCTCCTCCGC GATTGTCTGA GCAACGGAAC CACAATGGTT TTTTCAAGTT AACAGAGCCA
    GATGAAAACA TAAACACATT TTCAAGCGGA AGAAGAGGAG GCAGTCTCCG AGCTCAGGCA
    CCCAACGAGG ATCTTGATCT TGCTTTCGGC AGCTACGCAC CTTCTTTTGG TAAGTCCACC
    CCACGCGCAG GTTTGGGGAC ACGAAATGCA CAAAATCACT CCCCTCGAGA ACTTGATGAA
    GGGCTGGATC TGGGTGGCCT GGTCAAAGAG AGGAAGTCAA ACCTCATGCA GCAGCTTTTT
    GGGGCCACTG CAACTCCACC TCCTTCAGAC CCATCCAGCA AGATGGAGAT CCTGAGCCCT
    CCTCTAACAA CACAGTTTTC AGGACGCAGG AGGGACACAG ACACACCAAA TGAACTAAAC
    ATCAGTTCTC TCCCCAACAA CAGGAGCACT CTACAAGTCT CAGAGAGCCG ACCCGCGGTC
    AGGGCCATTA CATCATTTGA TGATGATATA GAGGAACTCA CACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021325689.1
    CDS399..2666
    Translation

    Target ORF information:

    RefSeq Version XM_021470014.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Leber congenital amaurosis 5 (lca5), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021470014.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGACTTGC ATGAAGATAA TCAGGAATCG GAGCACAGCC GATTGTCTCA CCATAATGAA 
    AGGAAGATCT TAGAATCCCT TCGATCTGGA AAGCAAGAGA ACAATGGAAA AGACATGGGG
    AACTCCGCTG GGGATCATGC ACAGAGCAGA ACTCGAGAGA CAGCTGGAGA TCCAGGCCGC
    AATGGGTTTT CAGATGGAGA GCGCAGCAGT GGCTCGTTCT ACTCTGATGA TTATGAAAAC
    GCCTCCCATT CAGATCGGTC ACTCTCACCG TCAGCTTCTC CATCGCCACC CAGAAGAGGG
    AGATCTAGGA GAGTTTCCAG CAGCCCTTTA CATAAGACAG GTGTGCACAA AGGTGCATCT
    CGCCGTATCC TGTCCCACTC CCATCATCCT CAGCAACGAG CAGGAGCAAT CCGATCCCAA
    AGTTTGAGCA AAGACGCTCC ATCCAAAGAA GTGGACCAGG TCACTAAACG CATGCTGTCA
    GCACGTCTAC TAAAGATCAA CGAGTTGAAG AACGCGTTGT CTGAGCTGAG GCTGCATGCG
    GACGAGCTGC AGCGAGAGAA CCGTTTACTG CGACAGCTGC AGCTCCGGCA AGAGAAAGCT
    TTGCACCGTT ACAATGACAC GGACAGCGAG ATCTCACAGC TGCTGGCTCG TCACAACAAT
    GAAACTCACG TGCTGCGCGA GAGATTGAGA CGCAGTCAAG AGAACCAACG CACAGCTGAG
    CGCAGCCTCA GGGAAAGAGA CGCTCAGCTC CAGCGCTGCC GCAGTCAAAT GCAGAAGCTG
    CAACAACTCG CAGATGACCA GAATCTTGGA GAGAGAGAGG AACTCTCGCG AAAACTAATT
    GGCACTCAAA GCAGATTGCA AGATAGTGAA CACAGAGTGA AGGAATTGGA GAAGAACATG
    GAGCTGAGTA GCGGGAGCTT TCAGAGGCAG CTTGCTAATG AGAGGAGGAA AACTCACGAT
    GCACAACAGG AAGTTAAAGC ACTGCAGGAG GAGGTCGAGA GACTTTGCAC TAAACTAAAG
    GAGAAAGAAA GGGAGCTGGA TGCCCGAAAC ATTTACGCTA ACAGGATGCA CAAAGCTTCT
    TCCAGGAAAG AAGCAGAGAA TGGCAGTAAA CGGAAAGGCT TTAATATAAC CAGCACTAAA
    GCAGTACAGA CAGAGGACAG AACATCCTCA TTGGATTTCC CATCGCCACC TCCAGCTCTT
    ACCAATAATC TTCCACCTGA TGACCAGACT GATGATTACC TTTCTCTGAA GCAGGATCAG
    CAAAGCAGAG AGCACAGGAC GAGAGAATCG AAGGAATGGA AGATTCAAGA GTTTTGGAGA
    GAAAGGGAGA AAGAAAGACA CATGGAATCT GCAAGAGAAC AGGAGAAAGA AAAAGAGACA
    CTGCATGAGA GAGAAAGACA AACAGAGCTG CTGAAAGATG TGGAGAGGCA AAGACTAGAT
    CAAGATCTCA GCTCAAATGA GAAGAACTTT AAAGGAAACA GAGTAGGCCT AAACAGAGAG
    GAGGAAGACA GGAGGATTGG CATGTCTGTG TCTCAAAAGG AAGAAGAGAT CCAAAAGCAG
    CGTGAACTGG AAGAGGAGCA ACAAAAAGTG AGAAATCGGG AGATCCAGGA CTACAATCAG
    GAGCGCGTGG AAGAAGAACG TCAGCGTAAA GAGCAGCTAT TAGCTAAGAT GCGTGAGATT
    GACATTCAAA CTCAAGGTCA GGACTCCGAC TTCTTTTCAG ATGACACAGG AAGTGTTCGC
    TCTCCTCCGC GATTGTCTGA GCAACGGAAC CACAATGGTT TTTTCAAGTT AACAGAGCCA
    GATGAAAACA TAAACACATT TTCAAGCGGA AGAAGAGGAG GCAGTCTCCG AGCTCAGGCA
    CCCAACGAGG ATCTTGATCT TGCTTTCGGC AGCTACGCAC CTTCTTTTGG TAAGTCCACC
    CCACGCGCAG GTTTGGGGAC ACGAAATGCA CAAAATCACT CCCCTCGAGA ACTTGATGAA
    GGGCTGGATC TGGGTGGCCT GGTCAAAGAG AGGAAGTCAA ACCTCATGCA GCAGCTTTTT
    GGGGCCACTG CAACTCCACC TCCTTCAGAC CCATCCAGCA AGATGGAGAT CCTGAGCCCT
    CCTCTAACAA CACAGTTTTC AGGACGCAGG AGGGACACAG ACACACCAAA TGAACTAAAC
    ATCAGTTCTC TCCCCAACAA CAGGAGCACT CTACAAGTCT CAGAGAGCCG ACCCGCGGTC
    AGGGCCATTA CATCATTTGA TGATGATATA GAGGAACTCA CACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa49001
    Clone ID Related Accession (Same CDS sequence) XM_021470014.1 , XM_009297008.2
    Accession Version XM_009297008.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2268bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product lebercilin isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297008.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGACTTGC ATGAAGATAA TCAGGAATCG GAGCACAGCC GATTGTCTCA CCATAATGAA 
    AGGAAGATCT TAGAATCCCT TCGATCTGGA AAGCAAGAGA ACAATGGAAA AGACATGGGG
    AACTCCGCTG GGGATCATGC ACAGAGCAGA ACTCGAGAGA CAGCTGGAGA TCCAGGCCGC
    AATGGGTTTT CAGATGGAGA GCGCAGCAGT GGCTCGTTCT ACTCTGATGA TTATGAAAAC
    GCCTCCCATT CAGATCGGTC ACTCTCACCG TCAGCTTCTC CATCGCCACC CAGAAGAGGG
    AGATCTAGGA GAGTTTCCAG CAGCCCTTTA CATAAGACAG GTGTGCACAA AGGTGCATCT
    CGCCGTATCC TGTCCCACTC CCATCATCCT CAGCAACGAG CAGGAGCAAT CCGATCCCAA
    AGTTTGAGCA AAGACGCTCC ATCCAAAGAA GTGGACCAGG TCACTAAACG CATGCTGTCA
    GCACGTCTAC TAAAGATCAA CGAGTTGAAG AACGCGTTGT CTGAGCTGAG GCTGCATGCG
    GACGAGCTGC AGCGAGAGAA CCGTTTACTG CGACAGCTGC AGCTCCGGCA AGAGAAAGCT
    TTGCACCGTT ACAATGACAC GGACAGCGAG ATCTCACAGC TGCTGGCTCG TCACAACAAT
    GAAACTCACG TGCTGCGCGA GAGATTGAGA CGCAGTCAAG AGAACCAACG CACAGCTGAG
    CGCAGCCTCA GGGAAAGAGA CGCTCAGCTC CAGCGCTGCC GCAGTCAAAT GCAGAAGCTG
    CAACAACTCG CAGATGACCA GAATCTTGGA GAGAGAGAGG AACTCTCGCG AAAACTAATT
    GGCACTCAAA GCAGATTGCA AGATAGTGAA CACAGAGTGA AGGAATTGGA GAAGAACATG
    GAGCTGAGTA GCGGGAGCTT TCAGAGGCAG CTTGCTAATG AGAGGAGGAA AACTCACGAT
    GCACAACAGG AAGTTAAAGC ACTGCAGGAG GAGGTCGAGA GACTTTGCAC TAAACTAAAG
    GAGAAAGAAA GGGAGCTGGA TGCCCGAAAC ATTTACGCTA ACAGGATGCA CAAAGCTTCT
    TCCAGGAAAG AAGCAGAGAA TGGCAGTAAA CGGAAAGGCT TTAATATAAC CAGCACTAAA
    GCAGTACAGA CAGAGGACAG AACATCCTCA TTGGATTTCC CATCGCCACC TCCAGCTCTT
    ACCAATAATC TTCCACCTGA TGACCAGACT GATGATTACC TTTCTCTGAA GCAGGATCAG
    CAAAGCAGAG AGCACAGGAC GAGAGAATCG AAGGAATGGA AGATTCAAGA GTTTTGGAGA
    GAAAGGGAGA AAGAAAGACA CATGGAATCT GCAAGAGAAC AGGAGAAAGA AAAAGAGACA
    CTGCATGAGA GAGAAAGACA AACAGAGCTG CTGAAAGATG TGGAGAGGCA AAGACTAGAT
    CAAGATCTCA GCTCAAATGA GAAGAACTTT AAAGGAAACA GAGTAGGCCT AAACAGAGAG
    GAGGAAGACA GGAGGATTGG CATGTCTGTG TCTCAAAAGG AAGAAGAGAT CCAAAAGCAG
    CGTGAACTGG AAGAGGAGCA ACAAAAAGTG AGAAATCGGG AGATCCAGGA CTACAATCAG
    GAGCGCGTGG AAGAAGAACG TCAGCGTAAA GAGCAGCTAT TAGCTAAGAT GCGTGAGATT
    GACATTCAAA CTCAAGGTCA GGACTCCGAC TTCTTTTCAG ATGACACAGG AAGTGTTCGC
    TCTCCTCCGC GATTGTCTGA GCAACGGAAC CACAATGGTT TTTTCAAGTT AACAGAGCCA
    GATGAAAACA TAAACACATT TTCAAGCGGA AGAAGAGGAG GCAGTCTCCG AGCTCAGGCA
    CCCAACGAGG ATCTTGATCT TGCTTTCGGC AGCTACGCAC CTTCTTTTGG TAAGTCCACC
    CCACGCGCAG GTTTGGGGAC ACGAAATGCA CAAAATCACT CCCCTCGAGA ACTTGATGAA
    GGGCTGGATC TGGGTGGCCT GGTCAAAGAG AGGAAGTCAA ACCTCATGCA GCAGCTTTTT
    GGGGCCACTG CAACTCCACC TCCTTCAGAC CCATCCAGCA AGATGGAGAT CCTGAGCCCT
    CCTCTAACAA CACAGTTTTC AGGACGCAGG AGGGACACAG ACACACCAAA TGAACTAAAC
    ATCAGTTCTC TCCCCAACAA CAGGAGCACT CTACAAGTCT CAGAGAGCCG ACCCGCGGTC
    AGGGCCATTA CATCATTTGA TGATGATATA GAGGAACTCA CACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295283.1
    CDS351..2618
    Translation

    Target ORF information:

    RefSeq Version XM_009297008.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Leber congenital amaurosis 5 (lca5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009297008.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGACTTGC ATGAAGATAA TCAGGAATCG GAGCACAGCC GATTGTCTCA CCATAATGAA 
    AGGAAGATCT TAGAATCCCT TCGATCTGGA AAGCAAGAGA ACAATGGAAA AGACATGGGG
    AACTCCGCTG GGGATCATGC ACAGAGCAGA ACTCGAGAGA CAGCTGGAGA TCCAGGCCGC
    AATGGGTTTT CAGATGGAGA GCGCAGCAGT GGCTCGTTCT ACTCTGATGA TTATGAAAAC
    GCCTCCCATT CAGATCGGTC ACTCTCACCG TCAGCTTCTC CATCGCCACC CAGAAGAGGG
    AGATCTAGGA GAGTTTCCAG CAGCCCTTTA CATAAGACAG GTGTGCACAA AGGTGCATCT
    CGCCGTATCC TGTCCCACTC CCATCATCCT CAGCAACGAG CAGGAGCAAT CCGATCCCAA
    AGTTTGAGCA AAGACGCTCC ATCCAAAGAA GTGGACCAGG TCACTAAACG CATGCTGTCA
    GCACGTCTAC TAAAGATCAA CGAGTTGAAG AACGCGTTGT CTGAGCTGAG GCTGCATGCG
    GACGAGCTGC AGCGAGAGAA CCGTTTACTG CGACAGCTGC AGCTCCGGCA AGAGAAAGCT
    TTGCACCGTT ACAATGACAC GGACAGCGAG ATCTCACAGC TGCTGGCTCG TCACAACAAT
    GAAACTCACG TGCTGCGCGA GAGATTGAGA CGCAGTCAAG AGAACCAACG CACAGCTGAG
    CGCAGCCTCA GGGAAAGAGA CGCTCAGCTC CAGCGCTGCC GCAGTCAAAT GCAGAAGCTG
    CAACAACTCG CAGATGACCA GAATCTTGGA GAGAGAGAGG AACTCTCGCG AAAACTAATT
    GGCACTCAAA GCAGATTGCA AGATAGTGAA CACAGAGTGA AGGAATTGGA GAAGAACATG
    GAGCTGAGTA GCGGGAGCTT TCAGAGGCAG CTTGCTAATG AGAGGAGGAA AACTCACGAT
    GCACAACAGG AAGTTAAAGC ACTGCAGGAG GAGGTCGAGA GACTTTGCAC TAAACTAAAG
    GAGAAAGAAA GGGAGCTGGA TGCCCGAAAC ATTTACGCTA ACAGGATGCA CAAAGCTTCT
    TCCAGGAAAG AAGCAGAGAA TGGCAGTAAA CGGAAAGGCT TTAATATAAC CAGCACTAAA
    GCAGTACAGA CAGAGGACAG AACATCCTCA TTGGATTTCC CATCGCCACC TCCAGCTCTT
    ACCAATAATC TTCCACCTGA TGACCAGACT GATGATTACC TTTCTCTGAA GCAGGATCAG
    CAAAGCAGAG AGCACAGGAC GAGAGAATCG AAGGAATGGA AGATTCAAGA GTTTTGGAGA
    GAAAGGGAGA AAGAAAGACA CATGGAATCT GCAAGAGAAC AGGAGAAAGA AAAAGAGACA
    CTGCATGAGA GAGAAAGACA AACAGAGCTG CTGAAAGATG TGGAGAGGCA AAGACTAGAT
    CAAGATCTCA GCTCAAATGA GAAGAACTTT AAAGGAAACA GAGTAGGCCT AAACAGAGAG
    GAGGAAGACA GGAGGATTGG CATGTCTGTG TCTCAAAAGG AAGAAGAGAT CCAAAAGCAG
    CGTGAACTGG AAGAGGAGCA ACAAAAAGTG AGAAATCGGG AGATCCAGGA CTACAATCAG
    GAGCGCGTGG AAGAAGAACG TCAGCGTAAA GAGCAGCTAT TAGCTAAGAT GCGTGAGATT
    GACATTCAAA CTCAAGGTCA GGACTCCGAC TTCTTTTCAG ATGACACAGG AAGTGTTCGC
    TCTCCTCCGC GATTGTCTGA GCAACGGAAC CACAATGGTT TTTTCAAGTT AACAGAGCCA
    GATGAAAACA TAAACACATT TTCAAGCGGA AGAAGAGGAG GCAGTCTCCG AGCTCAGGCA
    CCCAACGAGG ATCTTGATCT TGCTTTCGGC AGCTACGCAC CTTCTTTTGG TAAGTCCACC
    CCACGCGCAG GTTTGGGGAC ACGAAATGCA CAAAATCACT CCCCTCGAGA ACTTGATGAA
    GGGCTGGATC TGGGTGGCCT GGTCAAAGAG AGGAAGTCAA ACCTCATGCA GCAGCTTTTT
    GGGGCCACTG CAACTCCACC TCCTTCAGAC CCATCCAGCA AGATGGAGAT CCTGAGCCCT
    CCTCTAACAA CACAGTTTTC AGGACGCAGG AGGGACACAG ACACACCAAA TGAACTAAAC
    ATCAGTTCTC TCCCCAACAA CAGGAGCACT CTACAAGTCT CAGAGAGCCG ACCCGCGGTC
    AGGGCCATTA CATCATTTGA TGATGATATA GAGGAACTCA CACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa49002
    Clone ID Related Accession (Same CDS sequence) XM_009297009.2 , XM_021470015.1
    Accession Version XM_009297009.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2265bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product lebercilin isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297009.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGACTTGC ATGAAGATAA TCAGGAATCG GAGCACAGCC GATTGTCTCA CCATAATGAA 
    AGGAAGATCT TAGAATCCCT TCGATCTGGA AAGCAAGAGA ACAATGGAAA AGACATGGGG
    AACTCCGCTG GGGATCATGC ACAGAGCAGA ACTCGAGAGA CAGCTGGAGA TCCAGGCCGC
    AATGGGTTTT CAGATGGAGA GCGCAGCAGT GGCTCGTTCT ACTCTGATGA TTATGAAAAC
    GCCTCCCATT CAGATCGGTC ACTCTCACCG TCAGCTTCTC CATCGCCACC CAGAAGAGGG
    AGATCTAGGA GAGTTTCCAG CAGCCCTTTA CATAAGACAG GTGTGCACAA AGGTGCATCT
    CGCCGTATCC TGTCCCACTC CCATCATCCT CAGCAACGAG CAGGAGCAAT CCGATCCCAA
    AGTTTGAGCA AAGACGCTCC ATCCAAAGAA GTGGACCAGG TCACTAAACG CATGCTGTCA
    GCACGTCTAC TAAAGATCAA CGAGTTGAAG AACGCGTTGT CTGAGCTGAG GCTGCATGCG
    GACGAGCTGC AGCGAGAGAA CCGTTTACTG CGACAGCTGC AGCTCCGGCA AGAGAAAGCT
    TTGCACCGTT ACAATGACAC GGACAGCGAG ATCTCACAGC TGCTGGCTCG TCACAACAAT
    GAAACTCACG TGCTGCGCGA GAGATTGAGA CGCAGTCAAG AGAACCAACG CACAGCTGAG
    CGCAGCCTCA GGGAAAGAGA CGCTCAGCTC CAGCGCTGCC GCAGTCAAAT GCAGAAGCTG
    CAACAACTCG CAGATGACCA GAATCTTGGA GAGAGAGAGG AACTCTCGCG AAAACTAATT
    GGCACTCAAA GCAGATTGCA AGATAGTGAA CACAGAGTGA AGGAATTGGA GAAGAACATG
    GAGCTGAGTA GCGGGAGCTT TCAGAGGCAG CTTGCTAATG AGAGGAGGAA AACTCACGAT
    GCACAACAGG AAGTTAAAGC ACTGCAGGAG GAGGTCGAGA GACTTTGCAC TAAACTAAAG
    GAGAAAGAAA GGGAGCTGGA TGCCCGAAAC ATTTACGCTA ACAGGATGCA CAAAGCTTCT
    TCCAGGAAAG AAGCAGAGAA TGGCAGTAAA CGGAAAGGCT TTAATATAAC CAGCACTAAA
    GCAGTACAGA CAGAGGACAG AACATCCTCA TTGGATTTCC CATCGCCACC TCCAGCTCTT
    ACCAATAATC TTCCACCTGA TGACCAGACT GATGATTACC TTTCTCTGAA GGATCAGCAA
    AGCAGAGAGC ACAGGACGAG AGAATCGAAG GAATGGAAGA TTCAAGAGTT TTGGAGAGAA
    AGGGAGAAAG AAAGACACAT GGAATCTGCA AGAGAACAGG AGAAAGAAAA AGAGACACTG
    CATGAGAGAG AAAGACAAAC AGAGCTGCTG AAAGATGTGG AGAGGCAAAG ACTAGATCAA
    GATCTCAGCT CAAATGAGAA GAACTTTAAA GGAAACAGAG TAGGCCTAAA CAGAGAGGAG
    GAAGACAGGA GGATTGGCAT GTCTGTGTCT CAAAAGGAAG AAGAGATCCA AAAGCAGCGT
    GAACTGGAAG AGGAGCAACA AAAAGTGAGA AATCGGGAGA TCCAGGACTA CAATCAGGAG
    CGCGTGGAAG AAGAACGTCA GCGTAAAGAG CAGCTATTAG CTAAGATGCG TGAGATTGAC
    ATTCAAACTC AAGGTCAGGA CTCCGACTTC TTTTCAGATG ACACAGGAAG TGTTCGCTCT
    CCTCCGCGAT TGTCTGAGCA ACGGAACCAC AATGGTTTTT TCAAGTTAAC AGAGCCAGAT
    GAAAACATAA ACACATTTTC AAGCGGAAGA AGAGGAGGCA GTCTCCGAGC TCAGGCACCC
    AACGAGGATC TTGATCTTGC TTTCGGCAGC TACGCACCTT CTTTTGGTAA GTCCACCCCA
    CGCGCAGGTT TGGGGACACG AAATGCACAA AATCACTCCC CTCGAGAACT TGATGAAGGG
    CTGGATCTGG GTGGCCTGGT CAAAGAGAGG AAGTCAAACC TCATGCAGCA GCTTTTTGGG
    GCCACTGCAA CTCCACCTCC TTCAGACCCA TCCAGCAAGA TGGAGATCCT GAGCCCTCCT
    CTAACAACAC AGTTTTCAGG ACGCAGGAGG GACACAGACA CACCAAATGA ACTAAACATC
    AGTTCTCTCC CCAACAACAG GAGCACTCTA CAAGTCTCAG AGAGCCGACC CGCGGTCAGG
    GCCATTACAT CATTTGATGA TGATATAGAG GAACTCACAC TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295284.1
    CDS351..2615
    Translation

    Target ORF information:

    RefSeq Version XM_009297009.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Leber congenital amaurosis 5 (lca5), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009297009.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGACTTGC ATGAAGATAA TCAGGAATCG GAGCACAGCC GATTGTCTCA CCATAATGAA 
    AGGAAGATCT TAGAATCCCT TCGATCTGGA AAGCAAGAGA ACAATGGAAA AGACATGGGG
    AACTCCGCTG GGGATCATGC ACAGAGCAGA ACTCGAGAGA CAGCTGGAGA TCCAGGCCGC
    AATGGGTTTT CAGATGGAGA GCGCAGCAGT GGCTCGTTCT ACTCTGATGA TTATGAAAAC
    GCCTCCCATT CAGATCGGTC ACTCTCACCG TCAGCTTCTC CATCGCCACC CAGAAGAGGG
    AGATCTAGGA GAGTTTCCAG CAGCCCTTTA CATAAGACAG GTGTGCACAA AGGTGCATCT
    CGCCGTATCC TGTCCCACTC CCATCATCCT CAGCAACGAG CAGGAGCAAT CCGATCCCAA
    AGTTTGAGCA AAGACGCTCC ATCCAAAGAA GTGGACCAGG TCACTAAACG CATGCTGTCA
    GCACGTCTAC TAAAGATCAA CGAGTTGAAG AACGCGTTGT CTGAGCTGAG GCTGCATGCG
    GACGAGCTGC AGCGAGAGAA CCGTTTACTG CGACAGCTGC AGCTCCGGCA AGAGAAAGCT
    TTGCACCGTT ACAATGACAC GGACAGCGAG ATCTCACAGC TGCTGGCTCG TCACAACAAT
    GAAACTCACG TGCTGCGCGA GAGATTGAGA CGCAGTCAAG AGAACCAACG CACAGCTGAG
    CGCAGCCTCA GGGAAAGAGA CGCTCAGCTC CAGCGCTGCC GCAGTCAAAT GCAGAAGCTG
    CAACAACTCG CAGATGACCA GAATCTTGGA GAGAGAGAGG AACTCTCGCG AAAACTAATT
    GGCACTCAAA GCAGATTGCA AGATAGTGAA CACAGAGTGA AGGAATTGGA GAAGAACATG
    GAGCTGAGTA GCGGGAGCTT TCAGAGGCAG CTTGCTAATG AGAGGAGGAA AACTCACGAT
    GCACAACAGG AAGTTAAAGC ACTGCAGGAG GAGGTCGAGA GACTTTGCAC TAAACTAAAG
    GAGAAAGAAA GGGAGCTGGA TGCCCGAAAC ATTTACGCTA ACAGGATGCA CAAAGCTTCT
    TCCAGGAAAG AAGCAGAGAA TGGCAGTAAA CGGAAAGGCT TTAATATAAC CAGCACTAAA
    GCAGTACAGA CAGAGGACAG AACATCCTCA TTGGATTTCC CATCGCCACC TCCAGCTCTT
    ACCAATAATC TTCCACCTGA TGACCAGACT GATGATTACC TTTCTCTGAA GGATCAGCAA
    AGCAGAGAGC ACAGGACGAG AGAATCGAAG GAATGGAAGA TTCAAGAGTT TTGGAGAGAA
    AGGGAGAAAG AAAGACACAT GGAATCTGCA AGAGAACAGG AGAAAGAAAA AGAGACACTG
    CATGAGAGAG AAAGACAAAC AGAGCTGCTG AAAGATGTGG AGAGGCAAAG ACTAGATCAA
    GATCTCAGCT CAAATGAGAA GAACTTTAAA GGAAACAGAG TAGGCCTAAA CAGAGAGGAG
    GAAGACAGGA GGATTGGCAT GTCTGTGTCT CAAAAGGAAG AAGAGATCCA AAAGCAGCGT
    GAACTGGAAG AGGAGCAACA AAAAGTGAGA AATCGGGAGA TCCAGGACTA CAATCAGGAG
    CGCGTGGAAG AAGAACGTCA GCGTAAAGAG CAGCTATTAG CTAAGATGCG TGAGATTGAC
    ATTCAAACTC AAGGTCAGGA CTCCGACTTC TTTTCAGATG ACACAGGAAG TGTTCGCTCT
    CCTCCGCGAT TGTCTGAGCA ACGGAACCAC AATGGTTTTT TCAAGTTAAC AGAGCCAGAT
    GAAAACATAA ACACATTTTC AAGCGGAAGA AGAGGAGGCA GTCTCCGAGC TCAGGCACCC
    AACGAGGATC TTGATCTTGC TTTCGGCAGC TACGCACCTT CTTTTGGTAA GTCCACCCCA
    CGCGCAGGTT TGGGGACACG AAATGCACAA AATCACTCCC CTCGAGAACT TGATGAAGGG
    CTGGATCTGG GTGGCCTGGT CAAAGAGAGG AAGTCAAACC TCATGCAGCA GCTTTTTGGG
    GCCACTGCAA CTCCACCTCC TTCAGACCCA TCCAGCAAGA TGGAGATCCT GAGCCCTCCT
    CTAACAACAC AGTTTTCAGG ACGCAGGAGG GACACAGACA CACCAAATGA ACTAAACATC
    AGTTCTCTCC CCAACAACAG GAGCACTCTA CAAGTCTCAG AGAGCCGACC CGCGGTCAGG
    GCCATTACAT CATTTGATGA TGATATAGAG GAACTCACAC TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa49002
    Clone ID Related Accession (Same CDS sequence) XM_009297009.2 , XM_021470015.1
    Accession Version XM_021470015.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2265bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product lebercilin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGACTTGC ATGAAGATAA TCAGGAATCG GAGCACAGCC GATTGTCTCA CCATAATGAA 
    AGGAAGATCT TAGAATCCCT TCGATCTGGA AAGCAAGAGA ACAATGGAAA AGACATGGGG
    AACTCCGCTG GGGATCATGC ACAGAGCAGA ACTCGAGAGA CAGCTGGAGA TCCAGGCCGC
    AATGGGTTTT CAGATGGAGA GCGCAGCAGT GGCTCGTTCT ACTCTGATGA TTATGAAAAC
    GCCTCCCATT CAGATCGGTC ACTCTCACCG TCAGCTTCTC CATCGCCACC CAGAAGAGGG
    AGATCTAGGA GAGTTTCCAG CAGCCCTTTA CATAAGACAG GTGTGCACAA AGGTGCATCT
    CGCCGTATCC TGTCCCACTC CCATCATCCT CAGCAACGAG CAGGAGCAAT CCGATCCCAA
    AGTTTGAGCA AAGACGCTCC ATCCAAAGAA GTGGACCAGG TCACTAAACG CATGCTGTCA
    GCACGTCTAC TAAAGATCAA CGAGTTGAAG AACGCGTTGT CTGAGCTGAG GCTGCATGCG
    GACGAGCTGC AGCGAGAGAA CCGTTTACTG CGACAGCTGC AGCTCCGGCA AGAGAAAGCT
    TTGCACCGTT ACAATGACAC GGACAGCGAG ATCTCACAGC TGCTGGCTCG TCACAACAAT
    GAAACTCACG TGCTGCGCGA GAGATTGAGA CGCAGTCAAG AGAACCAACG CACAGCTGAG
    CGCAGCCTCA GGGAAAGAGA CGCTCAGCTC CAGCGCTGCC GCAGTCAAAT GCAGAAGCTG
    CAACAACTCG CAGATGACCA GAATCTTGGA GAGAGAGAGG AACTCTCGCG AAAACTAATT
    GGCACTCAAA GCAGATTGCA AGATAGTGAA CACAGAGTGA AGGAATTGGA GAAGAACATG
    GAGCTGAGTA GCGGGAGCTT TCAGAGGCAG CTTGCTAATG AGAGGAGGAA AACTCACGAT
    GCACAACAGG AAGTTAAAGC ACTGCAGGAG GAGGTCGAGA GACTTTGCAC TAAACTAAAG
    GAGAAAGAAA GGGAGCTGGA TGCCCGAAAC ATTTACGCTA ACAGGATGCA CAAAGCTTCT
    TCCAGGAAAG AAGCAGAGAA TGGCAGTAAA CGGAAAGGCT TTAATATAAC CAGCACTAAA
    GCAGTACAGA CAGAGGACAG AACATCCTCA TTGGATTTCC CATCGCCACC TCCAGCTCTT
    ACCAATAATC TTCCACCTGA TGACCAGACT GATGATTACC TTTCTCTGAA GGATCAGCAA
    AGCAGAGAGC ACAGGACGAG AGAATCGAAG GAATGGAAGA TTCAAGAGTT TTGGAGAGAA
    AGGGAGAAAG AAAGACACAT GGAATCTGCA AGAGAACAGG AGAAAGAAAA AGAGACACTG
    CATGAGAGAG AAAGACAAAC AGAGCTGCTG AAAGATGTGG AGAGGCAAAG ACTAGATCAA
    GATCTCAGCT CAAATGAGAA GAACTTTAAA GGAAACAGAG TAGGCCTAAA CAGAGAGGAG
    GAAGACAGGA GGATTGGCAT GTCTGTGTCT CAAAAGGAAG AAGAGATCCA AAAGCAGCGT
    GAACTGGAAG AGGAGCAACA AAAAGTGAGA AATCGGGAGA TCCAGGACTA CAATCAGGAG
    CGCGTGGAAG AAGAACGTCA GCGTAAAGAG CAGCTATTAG CTAAGATGCG TGAGATTGAC
    ATTCAAACTC AAGGTCAGGA CTCCGACTTC TTTTCAGATG ACACAGGAAG TGTTCGCTCT
    CCTCCGCGAT TGTCTGAGCA ACGGAACCAC AATGGTTTTT TCAAGTTAAC AGAGCCAGAT
    GAAAACATAA ACACATTTTC AAGCGGAAGA AGAGGAGGCA GTCTCCGAGC TCAGGCACCC
    AACGAGGATC TTGATCTTGC TTTCGGCAGC TACGCACCTT CTTTTGGTAA GTCCACCCCA
    CGCGCAGGTT TGGGGACACG AAATGCACAA AATCACTCCC CTCGAGAACT TGATGAAGGG
    CTGGATCTGG GTGGCCTGGT CAAAGAGAGG AAGTCAAACC TCATGCAGCA GCTTTTTGGG
    GCCACTGCAA CTCCACCTCC TTCAGACCCA TCCAGCAAGA TGGAGATCCT GAGCCCTCCT
    CTAACAACAC AGTTTTCAGG ACGCAGGAGG GACACAGACA CACCAAATGA ACTAAACATC
    AGTTCTCTCC CCAACAACAG GAGCACTCTA CAAGTCTCAG AGAGCCGACC CGCGGTCAGG
    GCCATTACAT CATTTGATGA TGATATAGAG GAACTCACAC TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021325690.1
    CDS398..2662
    Translation

    Target ORF information:

    RefSeq Version XM_021470015.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Leber congenital amaurosis 5 (lca5), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021470015.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGACTTGC ATGAAGATAA TCAGGAATCG GAGCACAGCC GATTGTCTCA CCATAATGAA 
    AGGAAGATCT TAGAATCCCT TCGATCTGGA AAGCAAGAGA ACAATGGAAA AGACATGGGG
    AACTCCGCTG GGGATCATGC ACAGAGCAGA ACTCGAGAGA CAGCTGGAGA TCCAGGCCGC
    AATGGGTTTT CAGATGGAGA GCGCAGCAGT GGCTCGTTCT ACTCTGATGA TTATGAAAAC
    GCCTCCCATT CAGATCGGTC ACTCTCACCG TCAGCTTCTC CATCGCCACC CAGAAGAGGG
    AGATCTAGGA GAGTTTCCAG CAGCCCTTTA CATAAGACAG GTGTGCACAA AGGTGCATCT
    CGCCGTATCC TGTCCCACTC CCATCATCCT CAGCAACGAG CAGGAGCAAT CCGATCCCAA
    AGTTTGAGCA AAGACGCTCC ATCCAAAGAA GTGGACCAGG TCACTAAACG CATGCTGTCA
    GCACGTCTAC TAAAGATCAA CGAGTTGAAG AACGCGTTGT CTGAGCTGAG GCTGCATGCG
    GACGAGCTGC AGCGAGAGAA CCGTTTACTG CGACAGCTGC AGCTCCGGCA AGAGAAAGCT
    TTGCACCGTT ACAATGACAC GGACAGCGAG ATCTCACAGC TGCTGGCTCG TCACAACAAT
    GAAACTCACG TGCTGCGCGA GAGATTGAGA CGCAGTCAAG AGAACCAACG CACAGCTGAG
    CGCAGCCTCA GGGAAAGAGA CGCTCAGCTC CAGCGCTGCC GCAGTCAAAT GCAGAAGCTG
    CAACAACTCG CAGATGACCA GAATCTTGGA GAGAGAGAGG AACTCTCGCG AAAACTAATT
    GGCACTCAAA GCAGATTGCA AGATAGTGAA CACAGAGTGA AGGAATTGGA GAAGAACATG
    GAGCTGAGTA GCGGGAGCTT TCAGAGGCAG CTTGCTAATG AGAGGAGGAA AACTCACGAT
    GCACAACAGG AAGTTAAAGC ACTGCAGGAG GAGGTCGAGA GACTTTGCAC TAAACTAAAG
    GAGAAAGAAA GGGAGCTGGA TGCCCGAAAC ATTTACGCTA ACAGGATGCA CAAAGCTTCT
    TCCAGGAAAG AAGCAGAGAA TGGCAGTAAA CGGAAAGGCT TTAATATAAC CAGCACTAAA
    GCAGTACAGA CAGAGGACAG AACATCCTCA TTGGATTTCC CATCGCCACC TCCAGCTCTT
    ACCAATAATC TTCCACCTGA TGACCAGACT GATGATTACC TTTCTCTGAA GGATCAGCAA
    AGCAGAGAGC ACAGGACGAG AGAATCGAAG GAATGGAAGA TTCAAGAGTT TTGGAGAGAA
    AGGGAGAAAG AAAGACACAT GGAATCTGCA AGAGAACAGG AGAAAGAAAA AGAGACACTG
    CATGAGAGAG AAAGACAAAC AGAGCTGCTG AAAGATGTGG AGAGGCAAAG ACTAGATCAA
    GATCTCAGCT CAAATGAGAA GAACTTTAAA GGAAACAGAG TAGGCCTAAA CAGAGAGGAG
    GAAGACAGGA GGATTGGCAT GTCTGTGTCT CAAAAGGAAG AAGAGATCCA AAAGCAGCGT
    GAACTGGAAG AGGAGCAACA AAAAGTGAGA AATCGGGAGA TCCAGGACTA CAATCAGGAG
    CGCGTGGAAG AAGAACGTCA GCGTAAAGAG CAGCTATTAG CTAAGATGCG TGAGATTGAC
    ATTCAAACTC AAGGTCAGGA CTCCGACTTC TTTTCAGATG ACACAGGAAG TGTTCGCTCT
    CCTCCGCGAT TGTCTGAGCA ACGGAACCAC AATGGTTTTT TCAAGTTAAC AGAGCCAGAT
    GAAAACATAA ACACATTTTC AAGCGGAAGA AGAGGAGGCA GTCTCCGAGC TCAGGCACCC
    AACGAGGATC TTGATCTTGC TTTCGGCAGC TACGCACCTT CTTTTGGTAA GTCCACCCCA
    CGCGCAGGTT TGGGGACACG AAATGCACAA AATCACTCCC CTCGAGAACT TGATGAAGGG
    CTGGATCTGG GTGGCCTGGT CAAAGAGAGG AAGTCAAACC TCATGCAGCA GCTTTTTGGG
    GCCACTGCAA CTCCACCTCC TTCAGACCCA TCCAGCAAGA TGGAGATCCT GAGCCCTCCT
    CTAACAACAC AGTTTTCAGG ACGCAGGAGG GACACAGACA CACCAAATGA ACTAAACATC
    AGTTCTCTCC CCAACAACAG GAGCACTCTA CAAGTCTCAG AGAGCCGACC CGCGGTCAGG
    GCCATTACAT CATTTGATGA TGATATAGAG GAACTCACAC TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146305
    Clone ID Related Accession (Same CDS sequence) XM_009297006.3 , XM_017353734.2
    Accession Version XM_009297006.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2304bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product lebercilin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395159.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009297006.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGACTTGC ATGAAGATAA TCAGGAATCG GAGCACAGCC GATTGTCTCA CCATAATGAA 
    AGGAAGATCT TAGAATCCCT TCGATCTGGA AAGCAAGAGA ACAATGGAAA AGACATGGGG
    AACTCCGCTG GGGATCATGC ACAGAGCAGA ACTCGAGAGA CAGCTGGAGA TCCAGACCGC
    AATGGGTTTT CAGATGGAGA GCGCAGCAGT GGCTCGTTCT ACTCTGATGA TTATGAAAAC
    GCCTCCCATT CAGATCGGTC ACTCTCACCG TCAGCTTCTC CATCGCCACC CAGAAGAGGG
    AGATCTAGGA GAGTTTCCAG CAGCCCTTTA CATAAGACAG GTGTGCACAA AGGTGCATCT
    CGCCGTATCC TGTCCCACTC CCATCATCCT CAGCAACGAG CAGGAGCAAT CCGATCCCAA
    AGTTTGAGCA AAGACGCTCC ATCCAAAGAT GTGGACCAGG TCACTAAACG CATGCTGTCA
    GCACGTCTGC TAAAGATCAA CGAGTTGAAG AACGCGTTGT CTGAGCTGAG GCTGCATGCG
    GACGAGCTGC AGCGAGAGAA CCGTTTACTG CGACAGCTGC AGCTCCGGCA AGAGAAAGCT
    TTGCACCGTT ACAATGACAC GGACAGCGAG ATCTCACAGC TGCTGGCTCG TCACAACAAT
    GAAACTCACG TGCTGCGCGA GAGATTGAGA CGCAGTCAAG AGAACCAACG CACAGCTGAG
    CGCAGCCTCA GGGAAAGAGA CGCTCAGCTC CAGCGCTGCC GCAGTCAAAT GCAGAAGCTG
    CAACAACTCG CAGATGACCA GAATCTTGGA GAGAGAGAGG AACTCTCGCG AAAACTAATT
    GGCACTCAAA GCAGATTGCA AGATAGTGAA CACAGAGTGA AGGAATTGGA GAAGAACATG
    GAGCTGAGTA GCGGGAGCTT TCAGAGGCAG CTTGCTAATG AGAGGAGGAA AACTCACGAT
    GCACAACAGG AAGTTAAAGC ACTGCAGGAG GAGGTCGAGA GACTTTGCAC AAAACTAAAG
    GAGAAAGAAA GGGAGCTGGA TGCCCGAAAC ATTTACGCTA ACAGGATGCA TAAAGCTTCT
    TCCAGGAAAG AAGCAGAGAA TGGCAGTAAA CGGAAAGGCT TTAATATAAC CAGCACTAAA
    GCAGTACAGA CAGAGGACAG AACATCCTCT TTGGATTTCC CATCGCCACC TCCAGCTCTT
    ACCAATAATC TTCCACCTGA TGACCAGACT GATGATTACC TTTCTCTGAA GGTTCAAAAG
    CCTACTGTGA ATCACAGGGT AAAAAAGCAG GATCAGCAAA GCAGAGAGCA CAGGACGAGA
    GAATCGAAGG AATGGAAGAT TCAAGAGTTT TGGAGAGAAA GGGAGAAAGA AAGACACATG
    GAATCTGCAA GAGAACAGGA GAAAGAAAAA GAGACACTGC ATGAGAGAGA AAGACAAACA
    GAGCTGCTGA AAGATGTGGA GAGGCAAAGA CTAGATCAAG ATCTCAGCTC AAATGAGAAG
    AACTTTAAAG GAAACAGAGT AGGCCTAAAC AGAGAGGAGG AAGACAGGAG GATTGGCATG
    TCTGTGTCTC AAAAGGAAGA AGAGATCCAA AAACAGCGTG AACTGGAAGA GGAGCAACAA
    AAAGTAAGGA ATCGGGAGAT CCAGGACTAC AATCAGGAGC GTGTGGAAGA AGAACGTCAG
    CGCAAAGAGC AGCTATTAGC TAAGATGCGT GAGATTGACA TTCAAACTCA AGGTCAGGAC
    TCCGACTTCT TTTCAGATGA CACAGGAAGT GTTCGCTCTC CTCCGCGATT GTCTGAGCAA
    CGGAACCACA ATGGTTTTTT CAAGTTAACA GAGCCAGATG AAAACATAAA CACATTTTCA
    AGCGGAAGAA GAGGAGGCAG TCTCCGAGCT CAGGCACCCA ACGAGGATCT TGATCTTGCT
    TTCGGCAGCT ACGCACCTTC TTTTGGTAAA TCCACCCCAC GCGCAGGTTT GGGGACACGA
    AATGCACAAA ATCACTCCCC TCGAGAACTT GATGAAGGGC TGGATCTGGG TGGCCTGGTC
    AAAGAGAGGA AGTCAAACCT CATGCAGCAG CTTTTTGGGG CCACTGCAAC TCCACCTCCT
    TCAGACCCAT CCAGCAAGAT GGAGATCCTG AGCCCTCCTC TAACAACACA GTTTTCAGGA
    CGCAGGAGGG ACACAGACAC ACCAAATGAA CTAAACATCA GTTCTCTCCC CAACAACAGG
    AGCACTCTAC AAGTCTCAGA GAGCCGACCC GCGGTCAGGG CCATTACATC ATTTGATGAT
    GATATAGAGG AACTCACACT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295281.2
    CDS396..2699
    Translation

    Target ORF information:

    RefSeq Version XM_009297006.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Leber congenital amaurosis 5 (lca5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009297006.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGACTTGC ATGAAGATAA TCAGGAATCG GAGCACAGCC GATTGTCTCA CCATAATGAA 
    AGGAAGATCT TAGAATCCCT TCGATCTGGA AAGCAAGAGA ACAATGGAAA AGACATGGGG
    AACTCCGCTG GGGATCATGC ACAGAGCAGA ACTCGAGAGA CAGCTGGAGA TCCAGACCGC
    AATGGGTTTT CAGATGGAGA GCGCAGCAGT GGCTCGTTCT ACTCTGATGA TTATGAAAAC
    GCCTCCCATT CAGATCGGTC ACTCTCACCG TCAGCTTCTC CATCGCCACC CAGAAGAGGG
    AGATCTAGGA GAGTTTCCAG CAGCCCTTTA CATAAGACAG GTGTGCACAA AGGTGCATCT
    CGCCGTATCC TGTCCCACTC CCATCATCCT CAGCAACGAG CAGGAGCAAT CCGATCCCAA
    AGTTTGAGCA AAGACGCTCC ATCCAAAGAT GTGGACCAGG TCACTAAACG CATGCTGTCA
    GCACGTCTGC TAAAGATCAA CGAGTTGAAG AACGCGTTGT CTGAGCTGAG GCTGCATGCG
    GACGAGCTGC AGCGAGAGAA CCGTTTACTG CGACAGCTGC AGCTCCGGCA AGAGAAAGCT
    TTGCACCGTT ACAATGACAC GGACAGCGAG ATCTCACAGC TGCTGGCTCG TCACAACAAT
    GAAACTCACG TGCTGCGCGA GAGATTGAGA CGCAGTCAAG AGAACCAACG CACAGCTGAG
    CGCAGCCTCA GGGAAAGAGA CGCTCAGCTC CAGCGCTGCC GCAGTCAAAT GCAGAAGCTG
    CAACAACTCG CAGATGACCA GAATCTTGGA GAGAGAGAGG AACTCTCGCG AAAACTAATT
    GGCACTCAAA GCAGATTGCA AGATAGTGAA CACAGAGTGA AGGAATTGGA GAAGAACATG
    GAGCTGAGTA GCGGGAGCTT TCAGAGGCAG CTTGCTAATG AGAGGAGGAA AACTCACGAT
    GCACAACAGG AAGTTAAAGC ACTGCAGGAG GAGGTCGAGA GACTTTGCAC AAAACTAAAG
    GAGAAAGAAA GGGAGCTGGA TGCCCGAAAC ATTTACGCTA ACAGGATGCA TAAAGCTTCT
    TCCAGGAAAG AAGCAGAGAA TGGCAGTAAA CGGAAAGGCT TTAATATAAC CAGCACTAAA
    GCAGTACAGA CAGAGGACAG AACATCCTCT TTGGATTTCC CATCGCCACC TCCAGCTCTT
    ACCAATAATC TTCCACCTGA TGACCAGACT GATGATTACC TTTCTCTGAA GGTTCAAAAG
    CCTACTGTGA ATCACAGGGT AAAAAAGCAG GATCAGCAAA GCAGAGAGCA CAGGACGAGA
    GAATCGAAGG AATGGAAGAT TCAAGAGTTT TGGAGAGAAA GGGAGAAAGA AAGACACATG
    GAATCTGCAA GAGAACAGGA GAAAGAAAAA GAGACACTGC ATGAGAGAGA AAGACAAACA
    GAGCTGCTGA AAGATGTGGA GAGGCAAAGA CTAGATCAAG ATCTCAGCTC AAATGAGAAG
    AACTTTAAAG GAAACAGAGT AGGCCTAAAC AGAGAGGAGG AAGACAGGAG GATTGGCATG
    TCTGTGTCTC AAAAGGAAGA AGAGATCCAA AAACAGCGTG AACTGGAAGA GGAGCAACAA
    AAAGTAAGGA ATCGGGAGAT CCAGGACTAC AATCAGGAGC GTGTGGAAGA AGAACGTCAG
    CGCAAAGAGC AGCTATTAGC TAAGATGCGT GAGATTGACA TTCAAACTCA AGGTCAGGAC
    TCCGACTTCT TTTCAGATGA CACAGGAAGT GTTCGCTCTC CTCCGCGATT GTCTGAGCAA
    CGGAACCACA ATGGTTTTTT CAAGTTAACA GAGCCAGATG AAAACATAAA CACATTTTCA
    AGCGGAAGAA GAGGAGGCAG TCTCCGAGCT CAGGCACCCA ACGAGGATCT TGATCTTGCT
    TTCGGCAGCT ACGCACCTTC TTTTGGTAAA TCCACCCCAC GCGCAGGTTT GGGGACACGA
    AATGCACAAA ATCACTCCCC TCGAGAACTT GATGAAGGGC TGGATCTGGG TGGCCTGGTC
    AAAGAGAGGA AGTCAAACCT CATGCAGCAG CTTTTTGGGG CCACTGCAAC TCCACCTCCT
    TCAGACCCAT CCAGCAAGAT GGAGATCCTG AGCCCTCCTC TAACAACACA GTTTTCAGGA
    CGCAGGAGGG ACACAGACAC ACCAAATGAA CTAAACATCA GTTCTCTCCC CAACAACAGG
    AGCACTCTAC AAGTCTCAGA GAGCCGACCC GCGGTCAGGG CCATTACATC ATTTGATGAT
    GATATAGAGG AACTCACACT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146305
    Clone ID Related Accession (Same CDS sequence) XM_009297006.3 , XM_017353734.2
    Accession Version XM_017353734.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2304bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product lebercilin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395159.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017353734.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGACTTGC ATGAAGATAA TCAGGAATCG GAGCACAGCC GATTGTCTCA CCATAATGAA 
    AGGAAGATCT TAGAATCCCT TCGATCTGGA AAGCAAGAGA ACAATGGAAA AGACATGGGG
    AACTCCGCTG GGGATCATGC ACAGAGCAGA ACTCGAGAGA CAGCTGGAGA TCCAGACCGC
    AATGGGTTTT CAGATGGAGA GCGCAGCAGT GGCTCGTTCT ACTCTGATGA TTATGAAAAC
    GCCTCCCATT CAGATCGGTC ACTCTCACCG TCAGCTTCTC CATCGCCACC CAGAAGAGGG
    AGATCTAGGA GAGTTTCCAG CAGCCCTTTA CATAAGACAG GTGTGCACAA AGGTGCATCT
    CGCCGTATCC TGTCCCACTC CCATCATCCT CAGCAACGAG CAGGAGCAAT CCGATCCCAA
    AGTTTGAGCA AAGACGCTCC ATCCAAAGAT GTGGACCAGG TCACTAAACG CATGCTGTCA
    GCACGTCTGC TAAAGATCAA CGAGTTGAAG AACGCGTTGT CTGAGCTGAG GCTGCATGCG
    GACGAGCTGC AGCGAGAGAA CCGTTTACTG CGACAGCTGC AGCTCCGGCA AGAGAAAGCT
    TTGCACCGTT ACAATGACAC GGACAGCGAG ATCTCACAGC TGCTGGCTCG TCACAACAAT
    GAAACTCACG TGCTGCGCGA GAGATTGAGA CGCAGTCAAG AGAACCAACG CACAGCTGAG
    CGCAGCCTCA GGGAAAGAGA CGCTCAGCTC CAGCGCTGCC GCAGTCAAAT GCAGAAGCTG
    CAACAACTCG CAGATGACCA GAATCTTGGA GAGAGAGAGG AACTCTCGCG AAAACTAATT
    GGCACTCAAA GCAGATTGCA AGATAGTGAA CACAGAGTGA AGGAATTGGA GAAGAACATG
    GAGCTGAGTA GCGGGAGCTT TCAGAGGCAG CTTGCTAATG AGAGGAGGAA AACTCACGAT
    GCACAACAGG AAGTTAAAGC ACTGCAGGAG GAGGTCGAGA GACTTTGCAC AAAACTAAAG
    GAGAAAGAAA GGGAGCTGGA TGCCCGAAAC ATTTACGCTA ACAGGATGCA TAAAGCTTCT
    TCCAGGAAAG AAGCAGAGAA TGGCAGTAAA CGGAAAGGCT TTAATATAAC CAGCACTAAA
    GCAGTACAGA CAGAGGACAG AACATCCTCT TTGGATTTCC CATCGCCACC TCCAGCTCTT
    ACCAATAATC TTCCACCTGA TGACCAGACT GATGATTACC TTTCTCTGAA GGTTCAAAAG
    CCTACTGTGA ATCACAGGGT AAAAAAGCAG GATCAGCAAA GCAGAGAGCA CAGGACGAGA
    GAATCGAAGG AATGGAAGAT TCAAGAGTTT TGGAGAGAAA GGGAGAAAGA AAGACACATG
    GAATCTGCAA GAGAACAGGA GAAAGAAAAA GAGACACTGC ATGAGAGAGA AAGACAAACA
    GAGCTGCTGA AAGATGTGGA GAGGCAAAGA CTAGATCAAG ATCTCAGCTC AAATGAGAAG
    AACTTTAAAG GAAACAGAGT AGGCCTAAAC AGAGAGGAGG AAGACAGGAG GATTGGCATG
    TCTGTGTCTC AAAAGGAAGA AGAGATCCAA AAACAGCGTG AACTGGAAGA GGAGCAACAA
    AAAGTAAGGA ATCGGGAGAT CCAGGACTAC AATCAGGAGC GTGTGGAAGA AGAACGTCAG
    CGCAAAGAGC AGCTATTAGC TAAGATGCGT GAGATTGACA TTCAAACTCA AGGTCAGGAC
    TCCGACTTCT TTTCAGATGA CACAGGAAGT GTTCGCTCTC CTCCGCGATT GTCTGAGCAA
    CGGAACCACA ATGGTTTTTT CAAGTTAACA GAGCCAGATG AAAACATAAA CACATTTTCA
    AGCGGAAGAA GAGGAGGCAG TCTCCGAGCT CAGGCACCCA ACGAGGATCT TGATCTTGCT
    TTCGGCAGCT ACGCACCTTC TTTTGGTAAA TCCACCCCAC GCGCAGGTTT GGGGACACGA
    AATGCACAAA ATCACTCCCC TCGAGAACTT GATGAAGGGC TGGATCTGGG TGGCCTGGTC
    AAAGAGAGGA AGTCAAACCT CATGCAGCAG CTTTTTGGGG CCACTGCAAC TCCACCTCCT
    TCAGACCCAT CCAGCAAGAT GGAGATCCTG AGCCCTCCTC TAACAACACA GTTTTCAGGA
    CGCAGGAGGG ACACAGACAC ACCAAATGAA CTAAACATCA GTTCTCTCCC CAACAACAGG
    AGCACTCTAC AAGTCTCAGA GAGCCGACCC GCGGTCAGGG CCATTACATC ATTTGATGAT
    GATATAGAGG AACTCACACT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017209223.2
    CDS394..2697
    Translation

    Target ORF information:

    RefSeq Version XM_017353734.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Leber congenital amaurosis 5 (lca5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017353734.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGACTTGC ATGAAGATAA TCAGGAATCG GAGCACAGCC GATTGTCTCA CCATAATGAA 
    AGGAAGATCT TAGAATCCCT TCGATCTGGA AAGCAAGAGA ACAATGGAAA AGACATGGGG
    AACTCCGCTG GGGATCATGC ACAGAGCAGA ACTCGAGAGA CAGCTGGAGA TCCAGACCGC
    AATGGGTTTT CAGATGGAGA GCGCAGCAGT GGCTCGTTCT ACTCTGATGA TTATGAAAAC
    GCCTCCCATT CAGATCGGTC ACTCTCACCG TCAGCTTCTC CATCGCCACC CAGAAGAGGG
    AGATCTAGGA GAGTTTCCAG CAGCCCTTTA CATAAGACAG GTGTGCACAA AGGTGCATCT
    CGCCGTATCC TGTCCCACTC CCATCATCCT CAGCAACGAG CAGGAGCAAT CCGATCCCAA
    AGTTTGAGCA AAGACGCTCC ATCCAAAGAT GTGGACCAGG TCACTAAACG CATGCTGTCA
    GCACGTCTGC TAAAGATCAA CGAGTTGAAG AACGCGTTGT CTGAGCTGAG GCTGCATGCG
    GACGAGCTGC AGCGAGAGAA CCGTTTACTG CGACAGCTGC AGCTCCGGCA AGAGAAAGCT
    TTGCACCGTT ACAATGACAC GGACAGCGAG ATCTCACAGC TGCTGGCTCG TCACAACAAT
    GAAACTCACG TGCTGCGCGA GAGATTGAGA CGCAGTCAAG AGAACCAACG CACAGCTGAG
    CGCAGCCTCA GGGAAAGAGA CGCTCAGCTC CAGCGCTGCC GCAGTCAAAT GCAGAAGCTG
    CAACAACTCG CAGATGACCA GAATCTTGGA GAGAGAGAGG AACTCTCGCG AAAACTAATT
    GGCACTCAAA GCAGATTGCA AGATAGTGAA CACAGAGTGA AGGAATTGGA GAAGAACATG
    GAGCTGAGTA GCGGGAGCTT TCAGAGGCAG CTTGCTAATG AGAGGAGGAA AACTCACGAT
    GCACAACAGG AAGTTAAAGC ACTGCAGGAG GAGGTCGAGA GACTTTGCAC AAAACTAAAG
    GAGAAAGAAA GGGAGCTGGA TGCCCGAAAC ATTTACGCTA ACAGGATGCA TAAAGCTTCT
    TCCAGGAAAG AAGCAGAGAA TGGCAGTAAA CGGAAAGGCT TTAATATAAC CAGCACTAAA
    GCAGTACAGA CAGAGGACAG AACATCCTCT TTGGATTTCC CATCGCCACC TCCAGCTCTT
    ACCAATAATC TTCCACCTGA TGACCAGACT GATGATTACC TTTCTCTGAA GGTTCAAAAG
    CCTACTGTGA ATCACAGGGT AAAAAAGCAG GATCAGCAAA GCAGAGAGCA CAGGACGAGA
    GAATCGAAGG AATGGAAGAT TCAAGAGTTT TGGAGAGAAA GGGAGAAAGA AAGACACATG
    GAATCTGCAA GAGAACAGGA GAAAGAAAAA GAGACACTGC ATGAGAGAGA AAGACAAACA
    GAGCTGCTGA AAGATGTGGA GAGGCAAAGA CTAGATCAAG ATCTCAGCTC AAATGAGAAG
    AACTTTAAAG GAAACAGAGT AGGCCTAAAC AGAGAGGAGG AAGACAGGAG GATTGGCATG
    TCTGTGTCTC AAAAGGAAGA AGAGATCCAA AAACAGCGTG AACTGGAAGA GGAGCAACAA
    AAAGTAAGGA ATCGGGAGAT CCAGGACTAC AATCAGGAGC GTGTGGAAGA AGAACGTCAG
    CGCAAAGAGC AGCTATTAGC TAAGATGCGT GAGATTGACA TTCAAACTCA AGGTCAGGAC
    TCCGACTTCT TTTCAGATGA CACAGGAAGT GTTCGCTCTC CTCCGCGATT GTCTGAGCAA
    CGGAACCACA ATGGTTTTTT CAAGTTAACA GAGCCAGATG AAAACATAAA CACATTTTCA
    AGCGGAAGAA GAGGAGGCAG TCTCCGAGCT CAGGCACCCA ACGAGGATCT TGATCTTGCT
    TTCGGCAGCT ACGCACCTTC TTTTGGTAAA TCCACCCCAC GCGCAGGTTT GGGGACACGA
    AATGCACAAA ATCACTCCCC TCGAGAACTT GATGAAGGGC TGGATCTGGG TGGCCTGGTC
    AAAGAGAGGA AGTCAAACCT CATGCAGCAG CTTTTTGGGG CCACTGCAAC TCCACCTCCT
    TCAGACCCAT CCAGCAAGAT GGAGATCCTG AGCCCTCCTC TAACAACACA GTTTTCAGGA
    CGCAGGAGGG ACACAGACAC ACCAAATGAA CTAAACATCA GTTCTCTCCC CAACAACAGG
    AGCACTCTAC AAGTCTCAGA GAGCCGACCC GCGGTCAGGG CCATTACATC ATTTGATGAT
    GATATAGAGG AACTCACACT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146306
    Clone ID Related Accession (Same CDS sequence) XM_021473730.1
    Accession Version XM_021473730.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2301bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product lebercilin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395159.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGACTTGC ATGAAGATAA TCAGGAATCG GAGCACAGCC GATTGTCTCA CCATAATGAA 
    AGGAAGATCT TAGAATCCCT TCGATCTGGA AAGCAAGAGA ACAATGGAAA AGACATGGGG
    AACTCCGCTG GGGATCATGC ACAGAGCAGA ACTCGAGAGA CAGCTGGAGA TCCAGACCGC
    AATGGGTTTT CAGATGGAGA GCGCAGCAGT GGCTCGTTCT ACTCTGATGA TTATGAAAAC
    GCCTCCCATT CAGATCGGTC ACTCTCACCG TCAGCTTCTC CATCGCCACC CAGAAGAGGG
    AGATCTAGGA GAGTTTCCAG CAGCCCTTTA CATAAGACAG GTGTGCACAA AGGTGCATCT
    CGCCGTATCC TGTCCCACTC CCATCATCCT CAGCAACGAG CAGGAGCAAT CCGATCCCAA
    AGTTTGAGCA AAGACGCTCC ATCCAAAGAT GTGGACCAGG TCACTAAACG CATGCTGTCA
    GCACGTCTGC TAAAGATCAA CGAGTTGAAG AACGCGTTGT CTGAGCTGAG GCTGCATGCG
    GACGAGCTGC AGCGAGAGAA CCGTTTACTG CGACAGCTGC AGCTCCGGCA AGAGAAAGCT
    TTGCACCGTT ACAATGACAC GGACAGCGAG ATCTCACAGC TGCTGGCTCG TCACAACAAT
    GAAACTCACG TGCTGCGCGA GAGATTGAGA CGCAGTCAAG AGAACCAACG CACAGCTGAG
    CGCAGCCTCA GGGAAAGAGA CGCTCAGCTC CAGCGCTGCC GCAGTCAAAT GCAGAAGCTG
    CAACAACTCG CAGATGACCA GAATCTTGGA GAGAGAGAGG AACTCTCGCG AAAACTAATT
    GGCACTCAAA GCAGATTGCA AGATAGTGAA CACAGAGTGA AGGAATTGGA GAAGAACATG
    GAGCTGAGTA GCGGGAGCTT TCAGAGGCAG CTTGCTAATG AGAGGAGGAA AACTCACGAT
    GCACAACAGG AAGTTAAAGC ACTGCAGGAG GAGGTCGAGA GACTTTGCAC AAAACTAAAG
    GAGAAAGAAA GGGAGCTGGA TGCCCGAAAC ATTTACGCTA ACAGGATGCA TAAAGCTTCT
    TCCAGGAAAG AAGCAGAGAA TGGCAGTAAA CGGAAAGGCT TTAATATAAC CAGCACTAAA
    GCAGTACAGA CAGAGGACAG AACATCCTCT TTGGATTTCC CATCGCCACC TCCAGCTCTT
    ACCAATAATC TTCCACCTGA TGACCAGACT GATGATTACC TTTCTCTGAA GGTTCAAAAG
    CCTACTGTGA ATCACAGGGT AAAAAAGGAT CAGCAAAGCA GAGAGCACAG GACGAGAGAA
    TCGAAGGAAT GGAAGATTCA AGAGTTTTGG AGAGAAAGGG AGAAAGAAAG ACACATGGAA
    TCTGCAAGAG AACAGGAGAA AGAAAAAGAG ACACTGCATG AGAGAGAAAG ACAAACAGAG
    CTGCTGAAAG ATGTGGAGAG GCAAAGACTA GATCAAGATC TCAGCTCAAA TGAGAAGAAC
    TTTAAAGGAA ACAGAGTAGG CCTAAACAGA GAGGAGGAAG ACAGGAGGAT TGGCATGTCT
    GTGTCTCAAA AGGAAGAAGA GATCCAAAAA CAGCGTGAAC TGGAAGAGGA GCAACAAAAA
    GTAAGGAATC GGGAGATCCA GGACTACAAT CAGGAGCGTG TGGAAGAAGA ACGTCAGCGC
    AAAGAGCAGC TATTAGCTAA GATGCGTGAG ATTGACATTC AAACTCAAGG TCAGGACTCC
    GACTTCTTTT CAGATGACAC AGGAAGTGTT CGCTCTCCTC CGCGATTGTC TGAGCAACGG
    AACCACAATG GTTTTTTCAA GTTAACAGAG CCAGATGAAA ACATAAACAC ATTTTCAAGC
    GGAAGAAGAG GAGGCAGTCT CCGAGCTCAG GCACCCAACG AGGATCTTGA TCTTGCTTTC
    GGCAGCTACG CACCTTCTTT TGGTAAATCC ACCCCACGCG CAGGTTTGGG GACACGAAAT
    GCACAAAATC ACTCCCCTCG AGAACTTGAT GAAGGGCTGG ATCTGGGTGG CCTGGTCAAA
    GAGAGGAAGT CAAACCTCAT GCAGCAGCTT TTTGGGGCCA CTGCAACTCC ACCTCCTTCA
    GACCCATCCA GCAAGATGGA GATCCTGAGC CCTCCTCTAA CAACACAGTT TTCAGGACGC
    AGGAGGGACA CAGACACACC AAATGAACTA AACATCAGTT CTCTCCCCAA CAACAGGAGC
    ACTCTACAAG TCTCAGAGAG CCGACCCGCG GTCAGGGCCA TTACATCATT TGATGATGAT
    ATAGAGGAAC TCACACTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329405.1
    CDS397..2697
    Translation

    Target ORF information:

    RefSeq Version XM_021473730.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Leber congenital amaurosis 5 (lca5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021473730.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGACTTGC ATGAAGATAA TCAGGAATCG GAGCACAGCC GATTGTCTCA CCATAATGAA 
    AGGAAGATCT TAGAATCCCT TCGATCTGGA AAGCAAGAGA ACAATGGAAA AGACATGGGG
    AACTCCGCTG GGGATCATGC ACAGAGCAGA ACTCGAGAGA CAGCTGGAGA TCCAGACCGC
    AATGGGTTTT CAGATGGAGA GCGCAGCAGT GGCTCGTTCT ACTCTGATGA TTATGAAAAC
    GCCTCCCATT CAGATCGGTC ACTCTCACCG TCAGCTTCTC CATCGCCACC CAGAAGAGGG
    AGATCTAGGA GAGTTTCCAG CAGCCCTTTA CATAAGACAG GTGTGCACAA AGGTGCATCT
    CGCCGTATCC TGTCCCACTC CCATCATCCT CAGCAACGAG CAGGAGCAAT CCGATCCCAA
    AGTTTGAGCA AAGACGCTCC ATCCAAAGAT GTGGACCAGG TCACTAAACG CATGCTGTCA
    GCACGTCTGC TAAAGATCAA CGAGTTGAAG AACGCGTTGT CTGAGCTGAG GCTGCATGCG
    GACGAGCTGC AGCGAGAGAA CCGTTTACTG CGACAGCTGC AGCTCCGGCA AGAGAAAGCT
    TTGCACCGTT ACAATGACAC GGACAGCGAG ATCTCACAGC TGCTGGCTCG TCACAACAAT
    GAAACTCACG TGCTGCGCGA GAGATTGAGA CGCAGTCAAG AGAACCAACG CACAGCTGAG
    CGCAGCCTCA GGGAAAGAGA CGCTCAGCTC CAGCGCTGCC GCAGTCAAAT GCAGAAGCTG
    CAACAACTCG CAGATGACCA GAATCTTGGA GAGAGAGAGG AACTCTCGCG AAAACTAATT
    GGCACTCAAA GCAGATTGCA AGATAGTGAA CACAGAGTGA AGGAATTGGA GAAGAACATG
    GAGCTGAGTA GCGGGAGCTT TCAGAGGCAG CTTGCTAATG AGAGGAGGAA AACTCACGAT
    GCACAACAGG AAGTTAAAGC ACTGCAGGAG GAGGTCGAGA GACTTTGCAC AAAACTAAAG
    GAGAAAGAAA GGGAGCTGGA TGCCCGAAAC ATTTACGCTA ACAGGATGCA TAAAGCTTCT
    TCCAGGAAAG AAGCAGAGAA TGGCAGTAAA CGGAAAGGCT TTAATATAAC CAGCACTAAA
    GCAGTACAGA CAGAGGACAG AACATCCTCT TTGGATTTCC CATCGCCACC TCCAGCTCTT
    ACCAATAATC TTCCACCTGA TGACCAGACT GATGATTACC TTTCTCTGAA GGTTCAAAAG
    CCTACTGTGA ATCACAGGGT AAAAAAGGAT CAGCAAAGCA GAGAGCACAG GACGAGAGAA
    TCGAAGGAAT GGAAGATTCA AGAGTTTTGG AGAGAAAGGG AGAAAGAAAG ACACATGGAA
    TCTGCAAGAG AACAGGAGAA AGAAAAAGAG ACACTGCATG AGAGAGAAAG ACAAACAGAG
    CTGCTGAAAG ATGTGGAGAG GCAAAGACTA GATCAAGATC TCAGCTCAAA TGAGAAGAAC
    TTTAAAGGAA ACAGAGTAGG CCTAAACAGA GAGGAGGAAG ACAGGAGGAT TGGCATGTCT
    GTGTCTCAAA AGGAAGAAGA GATCCAAAAA CAGCGTGAAC TGGAAGAGGA GCAACAAAAA
    GTAAGGAATC GGGAGATCCA GGACTACAAT CAGGAGCGTG TGGAAGAAGA ACGTCAGCGC
    AAAGAGCAGC TATTAGCTAA GATGCGTGAG ATTGACATTC AAACTCAAGG TCAGGACTCC
    GACTTCTTTT CAGATGACAC AGGAAGTGTT CGCTCTCCTC CGCGATTGTC TGAGCAACGG
    AACCACAATG GTTTTTTCAA GTTAACAGAG CCAGATGAAA ACATAAACAC ATTTTCAAGC
    GGAAGAAGAG GAGGCAGTCT CCGAGCTCAG GCACCCAACG AGGATCTTGA TCTTGCTTTC
    GGCAGCTACG CACCTTCTTT TGGTAAATCC ACCCCACGCG CAGGTTTGGG GACACGAAAT
    GCACAAAATC ACTCCCCTCG AGAACTTGAT GAAGGGCTGG ATCTGGGTGG CCTGGTCAAA
    GAGAGGAAGT CAAACCTCAT GCAGCAGCTT TTTGGGGCCA CTGCAACTCC ACCTCCTTCA
    GACCCATCCA GCAAGATGGA GATCCTGAGC CCTCCTCTAA CAACACAGTT TTCAGGACGC
    AGGAGGGACA CAGACACACC AAATGAACTA AACATCAGTT CTCTCCCCAA CAACAGGAGC
    ACTCTACAAG TCTCAGAGAG CCGACCCGCG GTCAGGGCCA TTACATCATT TGATGATGAT
    ATAGAGGAAC TCACACTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146307
    Clone ID Related Accession (Same CDS sequence) XM_017353735.2
    Accession Version XM_017353735.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2301bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product lebercilin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395159.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017353735.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGACTTGC ATGAAGATAA TCAGGAATCG GAGCACAGCC GATTGTCTCA CCATAATGAA 
    AGGAAGATCT TAGAATCCCT TCGATCTGGA AAGCAAGAGA ACAATGGAAA AGACATGGGG
    AACTCCGCTG GGGATCATGC ACAGAGCAGA ACTCGAGAGA CAGCTGGAGA TCCAGACCGC
    AATGGGTTTT CAGATGGAGA GCGCAGCAGT GGCTCGTTCT ACTCTGATGA TTATGAAAAC
    GCCTCCCATT CAGATCGGTC ACTCTCACCG TCAGCTTCTC CATCGCCACC CAGAAGAGGG
    AGATCTAGGA GAGTTTCCAG CAGCCCTTTA CATAAGACAG GTGTGCACAA AGGTGCATCT
    CGCCGTATCC TGTCCCACTC CCATCATCCT CAGCAACGAG CAGGAGCAAT CCGATCCCAA
    AGTTTGAGCA AAGACGCTCC ATCCAAAGAT GTGGACCAGG TCACTAAACG CATGCTGTCA
    GCACGTCTGC TAAAGATCAA CGAGTTGAAG AACGCGTTGT CTGAGCTGAG GCTGCATGCG
    GACGAGCTGC AGCGAGAGAA CCGTTTACTG CGACAGCTGC AGCTCCGGCA AGAGAAAGCT
    TTGCACCGTT ACAATGACAC GGACAGCGAG ATCTCACAGC TGCTGGCTCG TCACAACAAT
    GAAACTCACG TGCTGCGCGA GAGATTGAGA CGCAGTCAAG AGAACCAACG CACAGCTGAG
    CGCAGCCTCA GGGAAAGAGA CGCTCAGCTC CAGCGCTGCC GCAGTCAAAT GCAGAAGCTG
    CAACAACTCG CAGATGACCA GAATCTTGGA GAGAGAGAGG AACTCTCGCG AAAACTAATT
    GGCACTCAAA GCAGATTGCA AGATAGTGAA CACAGAGTGA AGGAATTGGA GAAGAACATG
    GAGCTGAGTA GCGGGAGCTT TCAGAGGCAG CTTGCTAATG AGAGGAGGAA AACTCACGAT
    GCACAACAGG AAGTTAAAGC ACTGCAGGAG GAGGTCGAGA GACTTTGCAC AAAACTAAAG
    GAGAAAGAAA GGGAGCTGGA TGCCCGAAAC ATTTACGCTA ACAGGATGCA TAAAGCTTCT
    TCCAGGAAAG AAGCAGAGAA TGGCAGTAAA CGGAAAGGCT TTAATATAAC CAGCACTAAA
    GCAGTACAGA CAGAGGACAG AACATCCTCT TTGGATTTCC CATCGCCACC TCCAGCTCTT
    ACCAATAATC TTCCACCTGA TGACCAGACT GATGATTACC TTTCTCTGAA GGTTCAAAAG
    CCTACTGTGA ATCACAGGGT AAAAAAGCAG GATCAGCAAA GCAGAGAGCA CAGGACGAGA
    GAATCGAAGG AATGGAAGAT TCAAGAGTTT TGGAGAGAAA GGGAGAAAGA AAGACACATG
    GAATCTGCAA GAGAACAGGA GAAAGAAAAA GAGACACTGC ATGAGAGAGA AAGACAAACA
    GAGCTGCTGA AAGATGTGGA GAGGCAAAGA CTAGATCAAG ATCTCAGCTC AAATGAGAAG
    AACTTTAAAG GAAACAGAGG CCTAAACAGA GAGGAGGAAG ACAGGAGGAT TGGCATGTCT
    GTGTCTCAAA AGGAAGAAGA GATCCAAAAA CAGCGTGAAC TGGAAGAGGA GCAACAAAAA
    GTAAGGAATC GGGAGATCCA GGACTACAAT CAGGAGCGTG TGGAAGAAGA ACGTCAGCGC
    AAAGAGCAGC TATTAGCTAA GATGCGTGAG ATTGACATTC AAACTCAAGG TCAGGACTCC
    GACTTCTTTT CAGATGACAC AGGAAGTGTT CGCTCTCCTC CGCGATTGTC TGAGCAACGG
    AACCACAATG GTTTTTTCAA GTTAACAGAG CCAGATGAAA ACATAAACAC ATTTTCAAGC
    GGAAGAAGAG GAGGCAGTCT CCGAGCTCAG GCACCCAACG AGGATCTTGA TCTTGCTTTC
    GGCAGCTACG CACCTTCTTT TGGTAAATCC ACCCCACGCG CAGGTTTGGG GACACGAAAT
    GCACAAAATC ACTCCCCTCG AGAACTTGAT GAAGGGCTGG ATCTGGGTGG CCTGGTCAAA
    GAGAGGAAGT CAAACCTCAT GCAGCAGCTT TTTGGGGCCA CTGCAACTCC ACCTCCTTCA
    GACCCATCCA GCAAGATGGA GATCCTGAGC CCTCCTCTAA CAACACAGTT TTCAGGACGC
    AGGAGGGACA CAGACACACC AAATGAACTA AACATCAGTT CTCTCCCCAA CAACAGGAGC
    ACTCTACAAG TCTCAGAGAG CCGACCCGCG GTCAGGGCCA TTACATCATT TGATGATGAT
    ATAGAGGAAC TCACACTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017209224.2
    CDS400..2700
    Translation

    Target ORF information:

    RefSeq Version XM_017353735.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Leber congenital amaurosis 5 (lca5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017353735.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGACTTGC ATGAAGATAA TCAGGAATCG GAGCACAGCC GATTGTCTCA CCATAATGAA 
    AGGAAGATCT TAGAATCCCT TCGATCTGGA AAGCAAGAGA ACAATGGAAA AGACATGGGG
    AACTCCGCTG GGGATCATGC ACAGAGCAGA ACTCGAGAGA CAGCTGGAGA TCCAGACCGC
    AATGGGTTTT CAGATGGAGA GCGCAGCAGT GGCTCGTTCT ACTCTGATGA TTATGAAAAC
    GCCTCCCATT CAGATCGGTC ACTCTCACCG TCAGCTTCTC CATCGCCACC CAGAAGAGGG
    AGATCTAGGA GAGTTTCCAG CAGCCCTTTA CATAAGACAG GTGTGCACAA AGGTGCATCT
    CGCCGTATCC TGTCCCACTC CCATCATCCT CAGCAACGAG CAGGAGCAAT CCGATCCCAA
    AGTTTGAGCA AAGACGCTCC ATCCAAAGAT GTGGACCAGG TCACTAAACG CATGCTGTCA
    GCACGTCTGC TAAAGATCAA CGAGTTGAAG AACGCGTTGT CTGAGCTGAG GCTGCATGCG
    GACGAGCTGC AGCGAGAGAA CCGTTTACTG CGACAGCTGC AGCTCCGGCA AGAGAAAGCT
    TTGCACCGTT ACAATGACAC GGACAGCGAG ATCTCACAGC TGCTGGCTCG TCACAACAAT
    GAAACTCACG TGCTGCGCGA GAGATTGAGA CGCAGTCAAG AGAACCAACG CACAGCTGAG
    CGCAGCCTCA GGGAAAGAGA CGCTCAGCTC CAGCGCTGCC GCAGTCAAAT GCAGAAGCTG
    CAACAACTCG CAGATGACCA GAATCTTGGA GAGAGAGAGG AACTCTCGCG AAAACTAATT
    GGCACTCAAA GCAGATTGCA AGATAGTGAA CACAGAGTGA AGGAATTGGA GAAGAACATG
    GAGCTGAGTA GCGGGAGCTT TCAGAGGCAG CTTGCTAATG AGAGGAGGAA AACTCACGAT
    GCACAACAGG AAGTTAAAGC ACTGCAGGAG GAGGTCGAGA GACTTTGCAC AAAACTAAAG
    GAGAAAGAAA GGGAGCTGGA TGCCCGAAAC ATTTACGCTA ACAGGATGCA TAAAGCTTCT
    TCCAGGAAAG AAGCAGAGAA TGGCAGTAAA CGGAAAGGCT TTAATATAAC CAGCACTAAA
    GCAGTACAGA CAGAGGACAG AACATCCTCT TTGGATTTCC CATCGCCACC TCCAGCTCTT
    ACCAATAATC TTCCACCTGA TGACCAGACT GATGATTACC TTTCTCTGAA GGTTCAAAAG
    CCTACTGTGA ATCACAGGGT AAAAAAGCAG GATCAGCAAA GCAGAGAGCA CAGGACGAGA
    GAATCGAAGG AATGGAAGAT TCAAGAGTTT TGGAGAGAAA GGGAGAAAGA AAGACACATG
    GAATCTGCAA GAGAACAGGA GAAAGAAAAA GAGACACTGC ATGAGAGAGA AAGACAAACA
    GAGCTGCTGA AAGATGTGGA GAGGCAAAGA CTAGATCAAG ATCTCAGCTC AAATGAGAAG
    AACTTTAAAG GAAACAGAGG CCTAAACAGA GAGGAGGAAG ACAGGAGGAT TGGCATGTCT
    GTGTCTCAAA AGGAAGAAGA GATCCAAAAA CAGCGTGAAC TGGAAGAGGA GCAACAAAAA
    GTAAGGAATC GGGAGATCCA GGACTACAAT CAGGAGCGTG TGGAAGAAGA ACGTCAGCGC
    AAAGAGCAGC TATTAGCTAA GATGCGTGAG ATTGACATTC AAACTCAAGG TCAGGACTCC
    GACTTCTTTT CAGATGACAC AGGAAGTGTT CGCTCTCCTC CGCGATTGTC TGAGCAACGG
    AACCACAATG GTTTTTTCAA GTTAACAGAG CCAGATGAAA ACATAAACAC ATTTTCAAGC
    GGAAGAAGAG GAGGCAGTCT CCGAGCTCAG GCACCCAACG AGGATCTTGA TCTTGCTTTC
    GGCAGCTACG CACCTTCTTT TGGTAAATCC ACCCCACGCG CAGGTTTGGG GACACGAAAT
    GCACAAAATC ACTCCCCTCG AGAACTTGAT GAAGGGCTGG ATCTGGGTGG CCTGGTCAAA
    GAGAGGAAGT CAAACCTCAT GCAGCAGCTT TTTGGGGCCA CTGCAACTCC ACCTCCTTCA
    GACCCATCCA GCAAGATGGA GATCCTGAGC CCTCCTCTAA CAACACAGTT TTCAGGACGC
    AGGAGGGACA CAGACACACC AAATGAACTA AACATCAGTT CTCTCCCCAA CAACAGGAGC
    ACTCTACAAG TCTCAGAGAG CCGACCCGCG GTCAGGGCCA TTACATCATT TGATGATGAT
    ATAGAGGAAC TCACACTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146308
    Clone ID Related Accession (Same CDS sequence) XM_009297008.3
    Accession Version XM_009297008.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2268bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product lebercilin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395159.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009297008.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGACTTGC ATGAAGATAA TCAGGAATCG GAGCACAGCC GATTGTCTCA CCATAATGAA 
    AGGAAGATCT TAGAATCCCT TCGATCTGGA AAGCAAGAGA ACAATGGAAA AGACATGGGG
    AACTCCGCTG GGGATCATGC ACAGAGCAGA ACTCGAGAGA CAGCTGGAGA TCCAGACCGC
    AATGGGTTTT CAGATGGAGA GCGCAGCAGT GGCTCGTTCT ACTCTGATGA TTATGAAAAC
    GCCTCCCATT CAGATCGGTC ACTCTCACCG TCAGCTTCTC CATCGCCACC CAGAAGAGGG
    AGATCTAGGA GAGTTTCCAG CAGCCCTTTA CATAAGACAG GTGTGCACAA AGGTGCATCT
    CGCCGTATCC TGTCCCACTC CCATCATCCT CAGCAACGAG CAGGAGCAAT CCGATCCCAA
    AGTTTGAGCA AAGACGCTCC ATCCAAAGAT GTGGACCAGG TCACTAAACG CATGCTGTCA
    GCACGTCTGC TAAAGATCAA CGAGTTGAAG AACGCGTTGT CTGAGCTGAG GCTGCATGCG
    GACGAGCTGC AGCGAGAGAA CCGTTTACTG CGACAGCTGC AGCTCCGGCA AGAGAAAGCT
    TTGCACCGTT ACAATGACAC GGACAGCGAG ATCTCACAGC TGCTGGCTCG TCACAACAAT
    GAAACTCACG TGCTGCGCGA GAGATTGAGA CGCAGTCAAG AGAACCAACG CACAGCTGAG
    CGCAGCCTCA GGGAAAGAGA CGCTCAGCTC CAGCGCTGCC GCAGTCAAAT GCAGAAGCTG
    CAACAACTCG CAGATGACCA GAATCTTGGA GAGAGAGAGG AACTCTCGCG AAAACTAATT
    GGCACTCAAA GCAGATTGCA AGATAGTGAA CACAGAGTGA AGGAATTGGA GAAGAACATG
    GAGCTGAGTA GCGGGAGCTT TCAGAGGCAG CTTGCTAATG AGAGGAGGAA AACTCACGAT
    GCACAACAGG AAGTTAAAGC ACTGCAGGAG GAGGTCGAGA GACTTTGCAC AAAACTAAAG
    GAGAAAGAAA GGGAGCTGGA TGCCCGAAAC ATTTACGCTA ACAGGATGCA TAAAGCTTCT
    TCCAGGAAAG AAGCAGAGAA TGGCAGTAAA CGGAAAGGCT TTAATATAAC CAGCACTAAA
    GCAGTACAGA CAGAGGACAG AACATCCTCT TTGGATTTCC CATCGCCACC TCCAGCTCTT
    ACCAATAATC TTCCACCTGA TGACCAGACT GATGATTACC TTTCTCTGAA GCAGGATCAG
    CAAAGCAGAG AGCACAGGAC GAGAGAATCG AAGGAATGGA AGATTCAAGA GTTTTGGAGA
    GAAAGGGAGA AAGAAAGACA CATGGAATCT GCAAGAGAAC AGGAGAAAGA AAAAGAGACA
    CTGCATGAGA GAGAAAGACA AACAGAGCTG CTGAAAGATG TGGAGAGGCA AAGACTAGAT
    CAAGATCTCA GCTCAAATGA GAAGAACTTT AAAGGAAACA GAGTAGGCCT AAACAGAGAG
    GAGGAAGACA GGAGGATTGG CATGTCTGTG TCTCAAAAGG AAGAAGAGAT CCAAAAACAG
    CGTGAACTGG AAGAGGAGCA ACAAAAAGTA AGGAATCGGG AGATCCAGGA CTACAATCAG
    GAGCGTGTGG AAGAAGAACG TCAGCGCAAA GAGCAGCTAT TAGCTAAGAT GCGTGAGATT
    GACATTCAAA CTCAAGGTCA GGACTCCGAC TTCTTTTCAG ATGACACAGG AAGTGTTCGC
    TCTCCTCCGC GATTGTCTGA GCAACGGAAC CACAATGGTT TTTTCAAGTT AACAGAGCCA
    GATGAAAACA TAAACACATT TTCAAGCGGA AGAAGAGGAG GCAGTCTCCG AGCTCAGGCA
    CCCAACGAGG ATCTTGATCT TGCTTTCGGC AGCTACGCAC CTTCTTTTGG TAAATCCACC
    CCACGCGCAG GTTTGGGGAC ACGAAATGCA CAAAATCACT CCCCTCGAGA ACTTGATGAA
    GGGCTGGATC TGGGTGGCCT GGTCAAAGAG AGGAAGTCAA ACCTCATGCA GCAGCTTTTT
    GGGGCCACTG CAACTCCACC TCCTTCAGAC CCATCCAGCA AGATGGAGAT CCTGAGCCCT
    CCTCTAACAA CACAGTTTTC AGGACGCAGG AGGGACACAG ACACACCAAA TGAACTAAAC
    ATCAGTTCTC TCCCCAACAA CAGGAGCACT CTACAAGTCT CAGAGAGCCG ACCCGCGGTC
    AGGGCCATTA CATCATTTGA TGATGATATA GAGGAACTCA CACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295283.2
    CDS396..2663
    Translation

    Target ORF information:

    RefSeq Version XM_009297008.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Leber congenital amaurosis 5 (lca5), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009297008.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGACTTGC ATGAAGATAA TCAGGAATCG GAGCACAGCC GATTGTCTCA CCATAATGAA 
    AGGAAGATCT TAGAATCCCT TCGATCTGGA AAGCAAGAGA ACAATGGAAA AGACATGGGG
    AACTCCGCTG GGGATCATGC ACAGAGCAGA ACTCGAGAGA CAGCTGGAGA TCCAGACCGC
    AATGGGTTTT CAGATGGAGA GCGCAGCAGT GGCTCGTTCT ACTCTGATGA TTATGAAAAC
    GCCTCCCATT CAGATCGGTC ACTCTCACCG TCAGCTTCTC CATCGCCACC CAGAAGAGGG
    AGATCTAGGA GAGTTTCCAG CAGCCCTTTA CATAAGACAG GTGTGCACAA AGGTGCATCT
    CGCCGTATCC TGTCCCACTC CCATCATCCT CAGCAACGAG CAGGAGCAAT CCGATCCCAA
    AGTTTGAGCA AAGACGCTCC ATCCAAAGAT GTGGACCAGG TCACTAAACG CATGCTGTCA
    GCACGTCTGC TAAAGATCAA CGAGTTGAAG AACGCGTTGT CTGAGCTGAG GCTGCATGCG
    GACGAGCTGC AGCGAGAGAA CCGTTTACTG CGACAGCTGC AGCTCCGGCA AGAGAAAGCT
    TTGCACCGTT ACAATGACAC GGACAGCGAG ATCTCACAGC TGCTGGCTCG TCACAACAAT
    GAAACTCACG TGCTGCGCGA GAGATTGAGA CGCAGTCAAG AGAACCAACG CACAGCTGAG
    CGCAGCCTCA GGGAAAGAGA CGCTCAGCTC CAGCGCTGCC GCAGTCAAAT GCAGAAGCTG
    CAACAACTCG CAGATGACCA GAATCTTGGA GAGAGAGAGG AACTCTCGCG AAAACTAATT
    GGCACTCAAA GCAGATTGCA AGATAGTGAA CACAGAGTGA AGGAATTGGA GAAGAACATG
    GAGCTGAGTA GCGGGAGCTT TCAGAGGCAG CTTGCTAATG AGAGGAGGAA AACTCACGAT
    GCACAACAGG AAGTTAAAGC ACTGCAGGAG GAGGTCGAGA GACTTTGCAC AAAACTAAAG
    GAGAAAGAAA GGGAGCTGGA TGCCCGAAAC ATTTACGCTA ACAGGATGCA TAAAGCTTCT
    TCCAGGAAAG AAGCAGAGAA TGGCAGTAAA CGGAAAGGCT TTAATATAAC CAGCACTAAA
    GCAGTACAGA CAGAGGACAG AACATCCTCT TTGGATTTCC CATCGCCACC TCCAGCTCTT
    ACCAATAATC TTCCACCTGA TGACCAGACT GATGATTACC TTTCTCTGAA GCAGGATCAG
    CAAAGCAGAG AGCACAGGAC GAGAGAATCG AAGGAATGGA AGATTCAAGA GTTTTGGAGA
    GAAAGGGAGA AAGAAAGACA CATGGAATCT GCAAGAGAAC AGGAGAAAGA AAAAGAGACA
    CTGCATGAGA GAGAAAGACA AACAGAGCTG CTGAAAGATG TGGAGAGGCA AAGACTAGAT
    CAAGATCTCA GCTCAAATGA GAAGAACTTT AAAGGAAACA GAGTAGGCCT AAACAGAGAG
    GAGGAAGACA GGAGGATTGG CATGTCTGTG TCTCAAAAGG AAGAAGAGAT CCAAAAACAG
    CGTGAACTGG AAGAGGAGCA ACAAAAAGTA AGGAATCGGG AGATCCAGGA CTACAATCAG
    GAGCGTGTGG AAGAAGAACG TCAGCGCAAA GAGCAGCTAT TAGCTAAGAT GCGTGAGATT
    GACATTCAAA CTCAAGGTCA GGACTCCGAC TTCTTTTCAG ATGACACAGG AAGTGTTCGC
    TCTCCTCCGC GATTGTCTGA GCAACGGAAC CACAATGGTT TTTTCAAGTT AACAGAGCCA
    GATGAAAACA TAAACACATT TTCAAGCGGA AGAAGAGGAG GCAGTCTCCG AGCTCAGGCA
    CCCAACGAGG ATCTTGATCT TGCTTTCGGC AGCTACGCAC CTTCTTTTGG TAAATCCACC
    CCACGCGCAG GTTTGGGGAC ACGAAATGCA CAAAATCACT CCCCTCGAGA ACTTGATGAA
    GGGCTGGATC TGGGTGGCCT GGTCAAAGAG AGGAAGTCAA ACCTCATGCA GCAGCTTTTT
    GGGGCCACTG CAACTCCACC TCCTTCAGAC CCATCCAGCA AGATGGAGAT CCTGAGCCCT
    CCTCTAACAA CACAGTTTTC AGGACGCAGG AGGGACACAG ACACACCAAA TGAACTAAAC
    ATCAGTTCTC TCCCCAACAA CAGGAGCACT CTACAAGTCT CAGAGAGCCG ACCCGCGGTC
    AGGGCCATTA CATCATTTGA TGATGATATA GAGGAACTCA CACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146309
    Clone ID Related Accession (Same CDS sequence) XM_009297009.3
    Accession Version XM_009297009.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2265bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product lebercilin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395159.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009297009.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGACTTGC ATGAAGATAA TCAGGAATCG GAGCACAGCC GATTGTCTCA CCATAATGAA 
    AGGAAGATCT TAGAATCCCT TCGATCTGGA AAGCAAGAGA ACAATGGAAA AGACATGGGG
    AACTCCGCTG GGGATCATGC ACAGAGCAGA ACTCGAGAGA CAGCTGGAGA TCCAGACCGC
    AATGGGTTTT CAGATGGAGA GCGCAGCAGT GGCTCGTTCT ACTCTGATGA TTATGAAAAC
    GCCTCCCATT CAGATCGGTC ACTCTCACCG TCAGCTTCTC CATCGCCACC CAGAAGAGGG
    AGATCTAGGA GAGTTTCCAG CAGCCCTTTA CATAAGACAG GTGTGCACAA AGGTGCATCT
    CGCCGTATCC TGTCCCACTC CCATCATCCT CAGCAACGAG CAGGAGCAAT CCGATCCCAA
    AGTTTGAGCA AAGACGCTCC ATCCAAAGAT GTGGACCAGG TCACTAAACG CATGCTGTCA
    GCACGTCTGC TAAAGATCAA CGAGTTGAAG AACGCGTTGT CTGAGCTGAG GCTGCATGCG
    GACGAGCTGC AGCGAGAGAA CCGTTTACTG CGACAGCTGC AGCTCCGGCA AGAGAAAGCT
    TTGCACCGTT ACAATGACAC GGACAGCGAG ATCTCACAGC TGCTGGCTCG TCACAACAAT
    GAAACTCACG TGCTGCGCGA GAGATTGAGA CGCAGTCAAG AGAACCAACG CACAGCTGAG
    CGCAGCCTCA GGGAAAGAGA CGCTCAGCTC CAGCGCTGCC GCAGTCAAAT GCAGAAGCTG
    CAACAACTCG CAGATGACCA GAATCTTGGA GAGAGAGAGG AACTCTCGCG AAAACTAATT
    GGCACTCAAA GCAGATTGCA AGATAGTGAA CACAGAGTGA AGGAATTGGA GAAGAACATG
    GAGCTGAGTA GCGGGAGCTT TCAGAGGCAG CTTGCTAATG AGAGGAGGAA AACTCACGAT
    GCACAACAGG AAGTTAAAGC ACTGCAGGAG GAGGTCGAGA GACTTTGCAC AAAACTAAAG
    GAGAAAGAAA GGGAGCTGGA TGCCCGAAAC ATTTACGCTA ACAGGATGCA TAAAGCTTCT
    TCCAGGAAAG AAGCAGAGAA TGGCAGTAAA CGGAAAGGCT TTAATATAAC CAGCACTAAA
    GCAGTACAGA CAGAGGACAG AACATCCTCT TTGGATTTCC CATCGCCACC TCCAGCTCTT
    ACCAATAATC TTCCACCTGA TGACCAGACT GATGATTACC TTTCTCTGAA GGATCAGCAA
    AGCAGAGAGC ACAGGACGAG AGAATCGAAG GAATGGAAGA TTCAAGAGTT TTGGAGAGAA
    AGGGAGAAAG AAAGACACAT GGAATCTGCA AGAGAACAGG AGAAAGAAAA AGAGACACTG
    CATGAGAGAG AAAGACAAAC AGAGCTGCTG AAAGATGTGG AGAGGCAAAG ACTAGATCAA
    GATCTCAGCT CAAATGAGAA GAACTTTAAA GGAAACAGAG TAGGCCTAAA CAGAGAGGAG
    GAAGACAGGA GGATTGGCAT GTCTGTGTCT CAAAAGGAAG AAGAGATCCA AAAACAGCGT
    GAACTGGAAG AGGAGCAACA AAAAGTAAGG AATCGGGAGA TCCAGGACTA CAATCAGGAG
    CGTGTGGAAG AAGAACGTCA GCGCAAAGAG CAGCTATTAG CTAAGATGCG TGAGATTGAC
    ATTCAAACTC AAGGTCAGGA CTCCGACTTC TTTTCAGATG ACACAGGAAG TGTTCGCTCT
    CCTCCGCGAT TGTCTGAGCA ACGGAACCAC AATGGTTTTT TCAAGTTAAC AGAGCCAGAT
    GAAAACATAA ACACATTTTC AAGCGGAAGA AGAGGAGGCA GTCTCCGAGC TCAGGCACCC
    AACGAGGATC TTGATCTTGC TTTCGGCAGC TACGCACCTT CTTTTGGTAA ATCCACCCCA
    CGCGCAGGTT TGGGGACACG AAATGCACAA AATCACTCCC CTCGAGAACT TGATGAAGGG
    CTGGATCTGG GTGGCCTGGT CAAAGAGAGG AAGTCAAACC TCATGCAGCA GCTTTTTGGG
    GCCACTGCAA CTCCACCTCC TTCAGACCCA TCCAGCAAGA TGGAGATCCT GAGCCCTCCT
    CTAACAACAC AGTTTTCAGG ACGCAGGAGG GACACAGACA CACCAAATGA ACTAAACATC
    AGTTCTCTCC CCAACAACAG GAGCACTCTA CAAGTCTCAG AGAGCCGACC CGCGGTCAGG
    GCCATTACAT CATTTGATGA TGATATAGAG GAACTCACAC TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295284.2
    CDS396..2660
    Translation

    Target ORF information:

    RefSeq Version XM_009297009.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Leber congenital amaurosis 5 (lca5), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009297009.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGACTTGC ATGAAGATAA TCAGGAATCG GAGCACAGCC GATTGTCTCA CCATAATGAA 
    AGGAAGATCT TAGAATCCCT TCGATCTGGA AAGCAAGAGA ACAATGGAAA AGACATGGGG
    AACTCCGCTG GGGATCATGC ACAGAGCAGA ACTCGAGAGA CAGCTGGAGA TCCAGACCGC
    AATGGGTTTT CAGATGGAGA GCGCAGCAGT GGCTCGTTCT ACTCTGATGA TTATGAAAAC
    GCCTCCCATT CAGATCGGTC ACTCTCACCG TCAGCTTCTC CATCGCCACC CAGAAGAGGG
    AGATCTAGGA GAGTTTCCAG CAGCCCTTTA CATAAGACAG GTGTGCACAA AGGTGCATCT
    CGCCGTATCC TGTCCCACTC CCATCATCCT CAGCAACGAG CAGGAGCAAT CCGATCCCAA
    AGTTTGAGCA AAGACGCTCC ATCCAAAGAT GTGGACCAGG TCACTAAACG CATGCTGTCA
    GCACGTCTGC TAAAGATCAA CGAGTTGAAG AACGCGTTGT CTGAGCTGAG GCTGCATGCG
    GACGAGCTGC AGCGAGAGAA CCGTTTACTG CGACAGCTGC AGCTCCGGCA AGAGAAAGCT
    TTGCACCGTT ACAATGACAC GGACAGCGAG ATCTCACAGC TGCTGGCTCG TCACAACAAT
    GAAACTCACG TGCTGCGCGA GAGATTGAGA CGCAGTCAAG AGAACCAACG CACAGCTGAG
    CGCAGCCTCA GGGAAAGAGA CGCTCAGCTC CAGCGCTGCC GCAGTCAAAT GCAGAAGCTG
    CAACAACTCG CAGATGACCA GAATCTTGGA GAGAGAGAGG AACTCTCGCG AAAACTAATT
    GGCACTCAAA GCAGATTGCA AGATAGTGAA CACAGAGTGA AGGAATTGGA GAAGAACATG
    GAGCTGAGTA GCGGGAGCTT TCAGAGGCAG CTTGCTAATG AGAGGAGGAA AACTCACGAT
    GCACAACAGG AAGTTAAAGC ACTGCAGGAG GAGGTCGAGA GACTTTGCAC AAAACTAAAG
    GAGAAAGAAA GGGAGCTGGA TGCCCGAAAC ATTTACGCTA ACAGGATGCA TAAAGCTTCT
    TCCAGGAAAG AAGCAGAGAA TGGCAGTAAA CGGAAAGGCT TTAATATAAC CAGCACTAAA
    GCAGTACAGA CAGAGGACAG AACATCCTCT TTGGATTTCC CATCGCCACC TCCAGCTCTT
    ACCAATAATC TTCCACCTGA TGACCAGACT GATGATTACC TTTCTCTGAA GGATCAGCAA
    AGCAGAGAGC ACAGGACGAG AGAATCGAAG GAATGGAAGA TTCAAGAGTT TTGGAGAGAA
    AGGGAGAAAG AAAGACACAT GGAATCTGCA AGAGAACAGG AGAAAGAAAA AGAGACACTG
    CATGAGAGAG AAAGACAAAC AGAGCTGCTG AAAGATGTGG AGAGGCAAAG ACTAGATCAA
    GATCTCAGCT CAAATGAGAA GAACTTTAAA GGAAACAGAG TAGGCCTAAA CAGAGAGGAG
    GAAGACAGGA GGATTGGCAT GTCTGTGTCT CAAAAGGAAG AAGAGATCCA AAAACAGCGT
    GAACTGGAAG AGGAGCAACA AAAAGTAAGG AATCGGGAGA TCCAGGACTA CAATCAGGAG
    CGTGTGGAAG AAGAACGTCA GCGCAAAGAG CAGCTATTAG CTAAGATGCG TGAGATTGAC
    ATTCAAACTC AAGGTCAGGA CTCCGACTTC TTTTCAGATG ACACAGGAAG TGTTCGCTCT
    CCTCCGCGAT TGTCTGAGCA ACGGAACCAC AATGGTTTTT TCAAGTTAAC AGAGCCAGAT
    GAAAACATAA ACACATTTTC AAGCGGAAGA AGAGGAGGCA GTCTCCGAGC TCAGGCACCC
    AACGAGGATC TTGATCTTGC TTTCGGCAGC TACGCACCTT CTTTTGGTAA ATCCACCCCA
    CGCGCAGGTT TGGGGACACG AAATGCACAA AATCACTCCC CTCGAGAACT TGATGAAGGG
    CTGGATCTGG GTGGCCTGGT CAAAGAGAGG AAGTCAAACC TCATGCAGCA GCTTTTTGGG
    GCCACTGCAA CTCCACCTCC TTCAGACCCA TCCAGCAAGA TGGAGATCCT GAGCCCTCCT
    CTAACAACAC AGTTTTCAGG ACGCAGGAGG GACACAGACA CACCAAATGA ACTAAACATC
    AGTTCTCTCC CCAACAACAG GAGCACTCTA CAAGTCTCAG AGAGCCGACC CGCGGTCAGG
    GCCATTACAT CATTTGATGA TGATATAGAG GAACTCACAC TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146310
    Clone ID Related Accession (Same CDS sequence) XM_017353736.2
    Accession Version XM_017353736.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2262bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product lebercilin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395159.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017353736.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGACTTGC ATGAAGATAA TCAGGAATCG GAGCACAGCC GATTGTCTCA CCATAATGAA 
    AGGAAGATCT TAGAATCCCT TCGATCTGGA AAGCAAGAGA ACAATGGAAA AGACATGGGG
    AACTCCGCTG GGGATCATGC ACAGAGCAGA ACTCGAGAGA CAGCTGGAGA TCCAGACCGC
    AATGGGTTTT CAGATGGAGA GCGCAGCAGT GGCTCGTTCT ACTCTGATGA TTATGAAAAC
    GCCTCCCATT CAGATCGGTC ACTCTCACCG TCAGCTTCTC CATCGCCACC CAGAAGAGGG
    AGATCTAGGA GAGTTTCCAG CAGCCCTTTA CATAAGACAG GTGTGCACAA AGGTGCATCT
    CGCCGTATCC TGTCCCACTC CCATCATCCT CAGCAACGAG CAGGAGCAAT CCGATCCCAA
    AGTTTGAGCA AAGACGCTCC ATCCAAAGAT GTGGACCAGG TCACTAAACG CATGCTGTCA
    GCACGTCTGC TAAAGATCAA CGAGTTGAAG AACGCGTTGT CTGAGCTGAG GCTGCATGCG
    GACGAGCTGC AGCGAGAGAA CCGTTTACTG CGACAGCTGC AGCTCCGGCA AGAGAAAGCT
    TTGCACCGTT ACAATGACAC GGACAGCGAG ATCTCACAGC TGCTGGCTCG TCACAACAAT
    GAAACTCACG TGCTGCGCGA GAGATTGAGA CGCAGTCAAG AGAACCAACG CACAGCTGAG
    CGCAGCCTCA GGGAAAGAGA CGCTCAGCTC CAGCGCTGCC GCAGTCAAAT GCAGAAGCTG
    CAACAACTCG CAGATGACCA GAATCTTGGA GAGAGAGAGG AACTCTCGCG AAAACTAATT
    GGCACTCAAA GCAGATTGCA AGATAGTGAA CACAGAGTGA AGGAATTGGA GAAGAACATG
    GAGCTGAGTA GCGGGAGCTT TCAGAGGCAG CTTGCTAATG AGAGGAGGAA AACTCACGAT
    GCACAACAGG AAGTTAAAGC ACTGCAGGAG GAGGTCGAGA GACTTTGCAC AAAACTAAAG
    GAGAAAGAAA GGGAGCTGGA TGCCCGAAAC ATTTACGCTA ACAGGATGCA TAAAGCTTCT
    TCCAGGAAAG AAGCAGAGAA TGGCAGTAAA CGGAAAGGCT TTAATATAAC CAGCACTAAA
    GCAGTACAGA CAGAGGACAG AACATCCTCT TTGGATTTCC CATCGCCACC TCCAGCTCTT
    ACCAATAATC TTCCACCTGA TGACCAGACT GATGATTACC TTTCTCTGAA GGATCAGCAA
    AGCAGAGAGC ACAGGACGAG AGAATCGAAG GAATGGAAGA TTCAAGAGTT TTGGAGAGAA
    AGGGAGAAAG AAAGACACAT GGAATCTGCA AGAGAACAGG AGAAAGAAAA AGAGACACTG
    CATGAGAGAG AAAGACAAAC AGAGCTGCTG AAAGATGTGG AGAGGCAAAG ACTAGATCAA
    GATCTCAGCT CAAATGAGAA GAACTTTAAA GGAAACAGAG GCCTAAACAG AGAGGAGGAA
    GACAGGAGGA TTGGCATGTC TGTGTCTCAA AAGGAAGAAG AGATCCAAAA ACAGCGTGAA
    CTGGAAGAGG AGCAACAAAA AGTAAGGAAT CGGGAGATCC AGGACTACAA TCAGGAGCGT
    GTGGAAGAAG AACGTCAGCG CAAAGAGCAG CTATTAGCTA AGATGCGTGA GATTGACATT
    CAAACTCAAG GTCAGGACTC CGACTTCTTT TCAGATGACA CAGGAAGTGT TCGCTCTCCT
    CCGCGATTGT CTGAGCAACG GAACCACAAT GGTTTTTTCA AGTTAACAGA GCCAGATGAA
    AACATAAACA CATTTTCAAG CGGAAGAAGA GGAGGCAGTC TCCGAGCTCA GGCACCCAAC
    GAGGATCTTG ATCTTGCTTT CGGCAGCTAC GCACCTTCTT TTGGTAAATC CACCCCACGC
    GCAGGTTTGG GGACACGAAA TGCACAAAAT CACTCCCCTC GAGAACTTGA TGAAGGGCTG
    GATCTGGGTG GCCTGGTCAA AGAGAGGAAG TCAAACCTCA TGCAGCAGCT TTTTGGGGCC
    ACTGCAACTC CACCTCCTTC AGACCCATCC AGCAAGATGG AGATCCTGAG CCCTCCTCTA
    ACAACACAGT TTTCAGGACG CAGGAGGGAC ACAGACACAC CAAATGAACT AAACATCAGT
    TCTCTCCCCA ACAACAGGAG CACTCTACAA GTCTCAGAGA GCCGACCCGC GGTCAGGGCC
    ATTACATCAT TTGATGATGA TATAGAGGAA CTCACACTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017209225.2
    CDS400..2661
    Translation

    Target ORF information:

    RefSeq Version XM_017353736.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Leber congenital amaurosis 5 (lca5), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017353736.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGACTTGC ATGAAGATAA TCAGGAATCG GAGCACAGCC GATTGTCTCA CCATAATGAA 
    AGGAAGATCT TAGAATCCCT TCGATCTGGA AAGCAAGAGA ACAATGGAAA AGACATGGGG
    AACTCCGCTG GGGATCATGC ACAGAGCAGA ACTCGAGAGA CAGCTGGAGA TCCAGACCGC
    AATGGGTTTT CAGATGGAGA GCGCAGCAGT GGCTCGTTCT ACTCTGATGA TTATGAAAAC
    GCCTCCCATT CAGATCGGTC ACTCTCACCG TCAGCTTCTC CATCGCCACC CAGAAGAGGG
    AGATCTAGGA GAGTTTCCAG CAGCCCTTTA CATAAGACAG GTGTGCACAA AGGTGCATCT
    CGCCGTATCC TGTCCCACTC CCATCATCCT CAGCAACGAG CAGGAGCAAT CCGATCCCAA
    AGTTTGAGCA AAGACGCTCC ATCCAAAGAT GTGGACCAGG TCACTAAACG CATGCTGTCA
    GCACGTCTGC TAAAGATCAA CGAGTTGAAG AACGCGTTGT CTGAGCTGAG GCTGCATGCG
    GACGAGCTGC AGCGAGAGAA CCGTTTACTG CGACAGCTGC AGCTCCGGCA AGAGAAAGCT
    TTGCACCGTT ACAATGACAC GGACAGCGAG ATCTCACAGC TGCTGGCTCG TCACAACAAT
    GAAACTCACG TGCTGCGCGA GAGATTGAGA CGCAGTCAAG AGAACCAACG CACAGCTGAG
    CGCAGCCTCA GGGAAAGAGA CGCTCAGCTC CAGCGCTGCC GCAGTCAAAT GCAGAAGCTG
    CAACAACTCG CAGATGACCA GAATCTTGGA GAGAGAGAGG AACTCTCGCG AAAACTAATT
    GGCACTCAAA GCAGATTGCA AGATAGTGAA CACAGAGTGA AGGAATTGGA GAAGAACATG
    GAGCTGAGTA GCGGGAGCTT TCAGAGGCAG CTTGCTAATG AGAGGAGGAA AACTCACGAT
    GCACAACAGG AAGTTAAAGC ACTGCAGGAG GAGGTCGAGA GACTTTGCAC AAAACTAAAG
    GAGAAAGAAA GGGAGCTGGA TGCCCGAAAC ATTTACGCTA ACAGGATGCA TAAAGCTTCT
    TCCAGGAAAG AAGCAGAGAA TGGCAGTAAA CGGAAAGGCT TTAATATAAC CAGCACTAAA
    GCAGTACAGA CAGAGGACAG AACATCCTCT TTGGATTTCC CATCGCCACC TCCAGCTCTT
    ACCAATAATC TTCCACCTGA TGACCAGACT GATGATTACC TTTCTCTGAA GGATCAGCAA
    AGCAGAGAGC ACAGGACGAG AGAATCGAAG GAATGGAAGA TTCAAGAGTT TTGGAGAGAA
    AGGGAGAAAG AAAGACACAT GGAATCTGCA AGAGAACAGG AGAAAGAAAA AGAGACACTG
    CATGAGAGAG AAAGACAAAC AGAGCTGCTG AAAGATGTGG AGAGGCAAAG ACTAGATCAA
    GATCTCAGCT CAAATGAGAA GAACTTTAAA GGAAACAGAG GCCTAAACAG AGAGGAGGAA
    GACAGGAGGA TTGGCATGTC TGTGTCTCAA AAGGAAGAAG AGATCCAAAA ACAGCGTGAA
    CTGGAAGAGG AGCAACAAAA AGTAAGGAAT CGGGAGATCC AGGACTACAA TCAGGAGCGT
    GTGGAAGAAG AACGTCAGCG CAAAGAGCAG CTATTAGCTA AGATGCGTGA GATTGACATT
    CAAACTCAAG GTCAGGACTC CGACTTCTTT TCAGATGACA CAGGAAGTGT TCGCTCTCCT
    CCGCGATTGT CTGAGCAACG GAACCACAAT GGTTTTTTCA AGTTAACAGA GCCAGATGAA
    AACATAAACA CATTTTCAAG CGGAAGAAGA GGAGGCAGTC TCCGAGCTCA GGCACCCAAC
    GAGGATCTTG ATCTTGCTTT CGGCAGCTAC GCACCTTCTT TTGGTAAATC CACCCCACGC
    GCAGGTTTGG GGACACGAAA TGCACAAAAT CACTCCCCTC GAGAACTTGA TGAAGGGCTG
    GATCTGGGTG GCCTGGTCAA AGAGAGGAAG TCAAACCTCA TGCAGCAGCT TTTTGGGGCC
    ACTGCAACTC CACCTCCTTC AGACCCATCC AGCAAGATGG AGATCCTGAG CCCTCCTCTA
    ACAACACAGT TTTCAGGACG CAGGAGGGAC ACAGACACAC CAAATGAACT AAACATCAGT
    TCTCTCCCCA ACAACAGGAG CACTCTACAA GTCTCAGAGA GCCGACCCGC GGTCAGGGCC
    ATTACATCAT TTGATGATGA TATAGAGGAA CTCACACTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa49000
    Clone ID Related Accession (Same CDS sequence) XM_021470012.1
    Accession Version XM_021470012.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2301bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product lebercilin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGACTTGC ATGAAGATAA TCAGGAATCG GAGCACAGCC GATTGTCTCA CCATAATGAA 
    AGGAAGATCT TAGAATCCCT TCGATCTGGA AAGCAAGAGA ACAATGGAAA AGACATGGGG
    AACTCCGCTG GGGATCATGC ACAGAGCAGA ACTCGAGAGA CAGCTGGAGA TCCAGGCCGC
    AATGGGTTTT CAGATGGAGA GCGCAGCAGT GGCTCGTTCT ACTCTGATGA TTATGAAAAC
    GCCTCCCATT CAGATCGGTC ACTCTCACCG TCAGCTTCTC CATCGCCACC CAGAAGAGGG
    AGATCTAGGA GAGTTTCCAG CAGCCCTTTA CATAAGACAG GTGTGCACAA AGGTGCATCT
    CGCCGTATCC TGTCCCACTC CCATCATCCT CAGCAACGAG CAGGAGCAAT CCGATCCCAA
    AGTTTGAGCA AAGACGCTCC ATCCAAAGAA GTGGACCAGG TCACTAAACG CATGCTGTCA
    GCACGTCTAC TAAAGATCAA CGAGTTGAAG AACGCGTTGT CTGAGCTGAG GCTGCATGCG
    GACGAGCTGC AGCGAGAGAA CCGTTTACTG CGACAGCTGC AGCTCCGGCA AGAGAAAGCT
    TTGCACCGTT ACAATGACAC GGACAGCGAG ATCTCACAGC TGCTGGCTCG TCACAACAAT
    GAAACTCACG TGCTGCGCGA GAGATTGAGA CGCAGTCAAG AGAACCAACG CACAGCTGAG
    CGCAGCCTCA GGGAAAGAGA CGCTCAGCTC CAGCGCTGCC GCAGTCAAAT GCAGAAGCTG
    CAACAACTCG CAGATGACCA GAATCTTGGA GAGAGAGAGG AACTCTCGCG AAAACTAATT
    GGCACTCAAA GCAGATTGCA AGATAGTGAA CACAGAGTGA AGGAATTGGA GAAGAACATG
    GAGCTGAGTA GCGGGAGCTT TCAGAGGCAG CTTGCTAATG AGAGGAGGAA AACTCACGAT
    GCACAACAGG AAGTTAAAGC ACTGCAGGAG GAGGTCGAGA GACTTTGCAC TAAACTAAAG
    GAGAAAGAAA GGGAGCTGGA TGCCCGAAAC ATTTACGCTA ACAGGATGCA CAAAGCTTCT
    TCCAGGAAAG AAGCAGAGAA TGGCAGTAAA CGGAAAGGCT TTAATATAAC CAGCACTAAA
    GCAGTACAGA CAGAGGACAG AACATCCTCA TTGGATTTCC CATCGCCACC TCCAGCTCTT
    ACCAATAATC TTCCACCTGA TGACCAGACT GATGATTACC TTTCTCTGAA GGTTCAAAAG
    CCTACTGTGA ATCACAGGGT AAAAAAGGAT CAGCAAAGCA GAGAGCACAG GACGAGAGAA
    TCGAAGGAAT GGAAGATTCA AGAGTTTTGG AGAGAAAGGG AGAAAGAAAG ACACATGGAA
    TCTGCAAGAG AACAGGAGAA AGAAAAAGAG ACACTGCATG AGAGAGAAAG ACAAACAGAG
    CTGCTGAAAG ATGTGGAGAG GCAAAGACTA GATCAAGATC TCAGCTCAAA TGAGAAGAAC
    TTTAAAGGAA ACAGAGTAGG CCTAAACAGA GAGGAGGAAG ACAGGAGGAT TGGCATGTCT
    GTGTCTCAAA AGGAAGAAGA GATCCAAAAG CAGCGTGAAC TGGAAGAGGA GCAACAAAAA
    GTGAGAAATC GGGAGATCCA GGACTACAAT CAGGAGCGCG TGGAAGAAGA ACGTCAGCGT
    AAAGAGCAGC TATTAGCTAA GATGCGTGAG ATTGACATTC AAACTCAAGG TCAGGACTCC
    GACTTCTTTT CAGATGACAC AGGAAGTGTT CGCTCTCCTC CGCGATTGTC TGAGCAACGG
    AACCACAATG GTTTTTTCAA GTTAACAGAG CCAGATGAAA ACATAAACAC ATTTTCAAGC
    GGAAGAAGAG GAGGCAGTCT CCGAGCTCAG GCACCCAACG AGGATCTTGA TCTTGCTTTC
    GGCAGCTACG CACCTTCTTT TGGTAAGTCC ACCCCACGCG CAGGTTTGGG GACACGAAAT
    GCACAAAATC ACTCCCCTCG AGAACTTGAT GAAGGGCTGG ATCTGGGTGG CCTGGTCAAA
    GAGAGGAAGT CAAACCTCAT GCAGCAGCTT TTTGGGGCCA CTGCAACTCC ACCTCCTTCA
    GACCCATCCA GCAAGATGGA GATCCTGAGC CCTCCTCTAA CAACACAGTT TTCAGGACGC
    AGGAGGGACA CAGACACACC AAATGAACTA AACATCAGTT CTCTCCCCAA CAACAGGAGC
    ACTCTACAAG TCTCAGAGAG CCGACCCGCG GTCAGGGCCA TTACATCATT TGATGATGAT
    ATAGAGGAAC TCACACTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021325687.1
    CDS399..2699
    Translation

    Target ORF information:

    RefSeq Version XM_021470012.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Leber congenital amaurosis 5 (lca5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021470012.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGACTTGC ATGAAGATAA TCAGGAATCG GAGCACAGCC GATTGTCTCA CCATAATGAA 
    AGGAAGATCT TAGAATCCCT TCGATCTGGA AAGCAAGAGA ACAATGGAAA AGACATGGGG
    AACTCCGCTG GGGATCATGC ACAGAGCAGA ACTCGAGAGA CAGCTGGAGA TCCAGGCCGC
    AATGGGTTTT CAGATGGAGA GCGCAGCAGT GGCTCGTTCT ACTCTGATGA TTATGAAAAC
    GCCTCCCATT CAGATCGGTC ACTCTCACCG TCAGCTTCTC CATCGCCACC CAGAAGAGGG
    AGATCTAGGA GAGTTTCCAG CAGCCCTTTA CATAAGACAG GTGTGCACAA AGGTGCATCT
    CGCCGTATCC TGTCCCACTC CCATCATCCT CAGCAACGAG CAGGAGCAAT CCGATCCCAA
    AGTTTGAGCA AAGACGCTCC ATCCAAAGAA GTGGACCAGG TCACTAAACG CATGCTGTCA
    GCACGTCTAC TAAAGATCAA CGAGTTGAAG AACGCGTTGT CTGAGCTGAG GCTGCATGCG
    GACGAGCTGC AGCGAGAGAA CCGTTTACTG CGACAGCTGC AGCTCCGGCA AGAGAAAGCT
    TTGCACCGTT ACAATGACAC GGACAGCGAG ATCTCACAGC TGCTGGCTCG TCACAACAAT
    GAAACTCACG TGCTGCGCGA GAGATTGAGA CGCAGTCAAG AGAACCAACG CACAGCTGAG
    CGCAGCCTCA GGGAAAGAGA CGCTCAGCTC CAGCGCTGCC GCAGTCAAAT GCAGAAGCTG
    CAACAACTCG CAGATGACCA GAATCTTGGA GAGAGAGAGG AACTCTCGCG AAAACTAATT
    GGCACTCAAA GCAGATTGCA AGATAGTGAA CACAGAGTGA AGGAATTGGA GAAGAACATG
    GAGCTGAGTA GCGGGAGCTT TCAGAGGCAG CTTGCTAATG AGAGGAGGAA AACTCACGAT
    GCACAACAGG AAGTTAAAGC ACTGCAGGAG GAGGTCGAGA GACTTTGCAC TAAACTAAAG
    GAGAAAGAAA GGGAGCTGGA TGCCCGAAAC ATTTACGCTA ACAGGATGCA CAAAGCTTCT
    TCCAGGAAAG AAGCAGAGAA TGGCAGTAAA CGGAAAGGCT TTAATATAAC CAGCACTAAA
    GCAGTACAGA CAGAGGACAG AACATCCTCA TTGGATTTCC CATCGCCACC TCCAGCTCTT
    ACCAATAATC TTCCACCTGA TGACCAGACT GATGATTACC TTTCTCTGAA GGTTCAAAAG
    CCTACTGTGA ATCACAGGGT AAAAAAGGAT CAGCAAAGCA GAGAGCACAG GACGAGAGAA
    TCGAAGGAAT GGAAGATTCA AGAGTTTTGG AGAGAAAGGG AGAAAGAAAG ACACATGGAA
    TCTGCAAGAG AACAGGAGAA AGAAAAAGAG ACACTGCATG AGAGAGAAAG ACAAACAGAG
    CTGCTGAAAG ATGTGGAGAG GCAAAGACTA GATCAAGATC TCAGCTCAAA TGAGAAGAAC
    TTTAAAGGAA ACAGAGTAGG CCTAAACAGA GAGGAGGAAG ACAGGAGGAT TGGCATGTCT
    GTGTCTCAAA AGGAAGAAGA GATCCAAAAG CAGCGTGAAC TGGAAGAGGA GCAACAAAAA
    GTGAGAAATC GGGAGATCCA GGACTACAAT CAGGAGCGCG TGGAAGAAGA ACGTCAGCGT
    AAAGAGCAGC TATTAGCTAA GATGCGTGAG ATTGACATTC AAACTCAAGG TCAGGACTCC
    GACTTCTTTT CAGATGACAC AGGAAGTGTT CGCTCTCCTC CGCGATTGTC TGAGCAACGG
    AACCACAATG GTTTTTTCAA GTTAACAGAG CCAGATGAAA ACATAAACAC ATTTTCAAGC
    GGAAGAAGAG GAGGCAGTCT CCGAGCTCAG GCACCCAACG AGGATCTTGA TCTTGCTTTC
    GGCAGCTACG CACCTTCTTT TGGTAAGTCC ACCCCACGCG CAGGTTTGGG GACACGAAAT
    GCACAAAATC ACTCCCCTCG AGAACTTGAT GAAGGGCTGG ATCTGGGTGG CCTGGTCAAA
    GAGAGGAAGT CAAACCTCAT GCAGCAGCTT TTTGGGGCCA CTGCAACTCC ACCTCCTTCA
    GACCCATCCA GCAAGATGGA GATCCTGAGC CCTCCTCTAA CAACACAGTT TTCAGGACGC
    AGGAGGGACA CAGACACACC AAATGAACTA AACATCAGTT CTCTCCCCAA CAACAGGAGC
    ACTCTACAAG TCTCAGAGAG CCGACCCGCG GTCAGGGCCA TTACATCATT TGATGATGAT
    ATAGAGGAAC TCACACTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa57513
    Clone ID Related Accession (Same CDS sequence) XM_017353735.1 , XM_021470013.1
    Accession Version XM_017353735.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2301bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product lebercilin isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGACTTGC ATGAAGATAA TCAGGAATCG GAGCACAGCC GATTGTCTCA CCATAATGAA 
    AGGAAGATCT TAGAATCCCT TCGATCTGGA AAGCAAGAGA ACAATGGAAA AGACATGGGG
    AACTCCGCTG GGGATCATGC ACAGAGCAGA ACTCGAGAGA CAGCTGGAGA TCCAGGCCGC
    AATGGGTTTT CAGATGGAGA GCGCAGCAGT GGCTCGTTCT ACTCTGATGA TTATGAAAAC
    GCCTCCCATT CAGATCGGTC ACTCTCACCG TCAGCTTCTC CATCGCCACC CAGAAGAGGG
    AGATCTAGGA GAGTTTCCAG CAGCCCTTTA CATAAGACAG GTGTGCACAA AGGTGCATCT
    CGCCGTATCC TGTCCCACTC CCATCATCCT CAGCAACGAG CAGGAGCAAT CCGATCCCAA
    AGTTTGAGCA AAGACGCTCC ATCCAAAGAA GTGGACCAGG TCACTAAACG CATGCTGTCA
    GCACGTCTAC TAAAGATCAA CGAGTTGAAG AACGCGTTGT CTGAGCTGAG GCTGCATGCG
    GACGAGCTGC AGCGAGAGAA CCGTTTACTG CGACAGCTGC AGCTCCGGCA AGAGAAAGCT
    TTGCACCGTT ACAATGACAC GGACAGCGAG ATCTCACAGC TGCTGGCTCG TCACAACAAT
    GAAACTCACG TGCTGCGCGA GAGATTGAGA CGCAGTCAAG AGAACCAACG CACAGCTGAG
    CGCAGCCTCA GGGAAAGAGA CGCTCAGCTC CAGCGCTGCC GCAGTCAAAT GCAGAAGCTG
    CAACAACTCG CAGATGACCA GAATCTTGGA GAGAGAGAGG AACTCTCGCG AAAACTAATT
    GGCACTCAAA GCAGATTGCA AGATAGTGAA CACAGAGTGA AGGAATTGGA GAAGAACATG
    GAGCTGAGTA GCGGGAGCTT TCAGAGGCAG CTTGCTAATG AGAGGAGGAA AACTCACGAT
    GCACAACAGG AAGTTAAAGC ACTGCAGGAG GAGGTCGAGA GACTTTGCAC TAAACTAAAG
    GAGAAAGAAA GGGAGCTGGA TGCCCGAAAC ATTTACGCTA ACAGGATGCA CAAAGCTTCT
    TCCAGGAAAG AAGCAGAGAA TGGCAGTAAA CGGAAAGGCT TTAATATAAC CAGCACTAAA
    GCAGTACAGA CAGAGGACAG AACATCCTCA TTGGATTTCC CATCGCCACC TCCAGCTCTT
    ACCAATAATC TTCCACCTGA TGACCAGACT GATGATTACC TTTCTCTGAA GGTTCAAAAG
    CCTACTGTGA ATCACAGGGT AAAAAAGCAG GATCAGCAAA GCAGAGAGCA CAGGACGAGA
    GAATCGAAGG AATGGAAGAT TCAAGAGTTT TGGAGAGAAA GGGAGAAAGA AAGACACATG
    GAATCTGCAA GAGAACAGGA GAAAGAAAAA GAGACACTGC ATGAGAGAGA AAGACAAACA
    GAGCTGCTGA AAGATGTGGA GAGGCAAAGA CTAGATCAAG ATCTCAGCTC AAATGAGAAG
    AACTTTAAAG GAAACAGAGG CCTAAACAGA GAGGAGGAAG ACAGGAGGAT TGGCATGTCT
    GTGTCTCAAA AGGAAGAAGA GATCCAAAAG CAGCGTGAAC TGGAAGAGGA GCAACAAAAA
    GTGAGAAATC GGGAGATCCA GGACTACAAT CAGGAGCGCG TGGAAGAAGA ACGTCAGCGT
    AAAGAGCAGC TATTAGCTAA GATGCGTGAG ATTGACATTC AAACTCAAGG TCAGGACTCC
    GACTTCTTTT CAGATGACAC AGGAAGTGTT CGCTCTCCTC CGCGATTGTC TGAGCAACGG
    AACCACAATG GTTTTTTCAA GTTAACAGAG CCAGATGAAA ACATAAACAC ATTTTCAAGC
    GGAAGAAGAG GAGGCAGTCT CCGAGCTCAG GCACCCAACG AGGATCTTGA TCTTGCTTTC
    GGCAGCTACG CACCTTCTTT TGGTAAGTCC ACCCCACGCG CAGGTTTGGG GACACGAAAT
    GCACAAAATC ACTCCCCTCG AGAACTTGAT GAAGGGCTGG ATCTGGGTGG CCTGGTCAAA
    GAGAGGAAGT CAAACCTCAT GCAGCAGCTT TTTGGGGCCA CTGCAACTCC ACCTCCTTCA
    GACCCATCCA GCAAGATGGA GATCCTGAGC CCTCCTCTAA CAACACAGTT TTCAGGACGC
    AGGAGGGACA CAGACACACC AAATGAACTA AACATCAGTT CTCTCCCCAA CAACAGGAGC
    ACTCTACAAG TCTCAGAGAG CCGACCCGCG GTCAGGGCCA TTACATCATT TGATGATGAT
    ATAGAGGAAC TCACACTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017209224.1
    CDS351..2651
    Translation

    Target ORF information:

    RefSeq Version XM_017353735.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Leber congenital amaurosis 5 (lca5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017353735.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGACTTGC ATGAAGATAA TCAGGAATCG GAGCACAGCC GATTGTCTCA CCATAATGAA 
    AGGAAGATCT TAGAATCCCT TCGATCTGGA AAGCAAGAGA ACAATGGAAA AGACATGGGG
    AACTCCGCTG GGGATCATGC ACAGAGCAGA ACTCGAGAGA CAGCTGGAGA TCCAGGCCGC
    AATGGGTTTT CAGATGGAGA GCGCAGCAGT GGCTCGTTCT ACTCTGATGA TTATGAAAAC
    GCCTCCCATT CAGATCGGTC ACTCTCACCG TCAGCTTCTC CATCGCCACC CAGAAGAGGG
    AGATCTAGGA GAGTTTCCAG CAGCCCTTTA CATAAGACAG GTGTGCACAA AGGTGCATCT
    CGCCGTATCC TGTCCCACTC CCATCATCCT CAGCAACGAG CAGGAGCAAT CCGATCCCAA
    AGTTTGAGCA AAGACGCTCC ATCCAAAGAA GTGGACCAGG TCACTAAACG CATGCTGTCA
    GCACGTCTAC TAAAGATCAA CGAGTTGAAG AACGCGTTGT CTGAGCTGAG GCTGCATGCG
    GACGAGCTGC AGCGAGAGAA CCGTTTACTG CGACAGCTGC AGCTCCGGCA AGAGAAAGCT
    TTGCACCGTT ACAATGACAC GGACAGCGAG ATCTCACAGC TGCTGGCTCG TCACAACAAT
    GAAACTCACG TGCTGCGCGA GAGATTGAGA CGCAGTCAAG AGAACCAACG CACAGCTGAG
    CGCAGCCTCA GGGAAAGAGA CGCTCAGCTC CAGCGCTGCC GCAGTCAAAT GCAGAAGCTG
    CAACAACTCG CAGATGACCA GAATCTTGGA GAGAGAGAGG AACTCTCGCG AAAACTAATT
    GGCACTCAAA GCAGATTGCA AGATAGTGAA CACAGAGTGA AGGAATTGGA GAAGAACATG
    GAGCTGAGTA GCGGGAGCTT TCAGAGGCAG CTTGCTAATG AGAGGAGGAA AACTCACGAT
    GCACAACAGG AAGTTAAAGC ACTGCAGGAG GAGGTCGAGA GACTTTGCAC TAAACTAAAG
    GAGAAAGAAA GGGAGCTGGA TGCCCGAAAC ATTTACGCTA ACAGGATGCA CAAAGCTTCT
    TCCAGGAAAG AAGCAGAGAA TGGCAGTAAA CGGAAAGGCT TTAATATAAC CAGCACTAAA
    GCAGTACAGA CAGAGGACAG AACATCCTCA TTGGATTTCC CATCGCCACC TCCAGCTCTT
    ACCAATAATC TTCCACCTGA TGACCAGACT GATGATTACC TTTCTCTGAA GGTTCAAAAG
    CCTACTGTGA ATCACAGGGT AAAAAAGCAG GATCAGCAAA GCAGAGAGCA CAGGACGAGA
    GAATCGAAGG AATGGAAGAT TCAAGAGTTT TGGAGAGAAA GGGAGAAAGA AAGACACATG
    GAATCTGCAA GAGAACAGGA GAAAGAAAAA GAGACACTGC ATGAGAGAGA AAGACAAACA
    GAGCTGCTGA AAGATGTGGA GAGGCAAAGA CTAGATCAAG ATCTCAGCTC AAATGAGAAG
    AACTTTAAAG GAAACAGAGG CCTAAACAGA GAGGAGGAAG ACAGGAGGAT TGGCATGTCT
    GTGTCTCAAA AGGAAGAAGA GATCCAAAAG CAGCGTGAAC TGGAAGAGGA GCAACAAAAA
    GTGAGAAATC GGGAGATCCA GGACTACAAT CAGGAGCGCG TGGAAGAAGA ACGTCAGCGT
    AAAGAGCAGC TATTAGCTAA GATGCGTGAG ATTGACATTC AAACTCAAGG TCAGGACTCC
    GACTTCTTTT CAGATGACAC AGGAAGTGTT CGCTCTCCTC CGCGATTGTC TGAGCAACGG
    AACCACAATG GTTTTTTCAA GTTAACAGAG CCAGATGAAA ACATAAACAC ATTTTCAAGC
    GGAAGAAGAG GAGGCAGTCT CCGAGCTCAG GCACCCAACG AGGATCTTGA TCTTGCTTTC
    GGCAGCTACG CACCTTCTTT TGGTAAGTCC ACCCCACGCG CAGGTTTGGG GACACGAAAT
    GCACAAAATC ACTCCCCTCG AGAACTTGAT GAAGGGCTGG ATCTGGGTGG CCTGGTCAAA
    GAGAGGAAGT CAAACCTCAT GCAGCAGCTT TTTGGGGCCA CTGCAACTCC ACCTCCTTCA
    GACCCATCCA GCAAGATGGA GATCCTGAGC CCTCCTCTAA CAACACAGTT TTCAGGACGC
    AGGAGGGACA CAGACACACC AAATGAACTA AACATCAGTT CTCTCCCCAA CAACAGGAGC
    ACTCTACAAG TCTCAGAGAG CCGACCCGCG GTCAGGGCCA TTACATCATT TGATGATGAT
    ATAGAGGAAC TCACACTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa57513
    Clone ID Related Accession (Same CDS sequence) XM_017353735.1 , XM_021470013.1
    Accession Version XM_021470013.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2301bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product lebercilin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGACTTGC ATGAAGATAA TCAGGAATCG GAGCACAGCC GATTGTCTCA CCATAATGAA 
    AGGAAGATCT TAGAATCCCT TCGATCTGGA AAGCAAGAGA ACAATGGAAA AGACATGGGG
    AACTCCGCTG GGGATCATGC ACAGAGCAGA ACTCGAGAGA CAGCTGGAGA TCCAGGCCGC
    AATGGGTTTT CAGATGGAGA GCGCAGCAGT GGCTCGTTCT ACTCTGATGA TTATGAAAAC
    GCCTCCCATT CAGATCGGTC ACTCTCACCG TCAGCTTCTC CATCGCCACC CAGAAGAGGG
    AGATCTAGGA GAGTTTCCAG CAGCCCTTTA CATAAGACAG GTGTGCACAA AGGTGCATCT
    CGCCGTATCC TGTCCCACTC CCATCATCCT CAGCAACGAG CAGGAGCAAT CCGATCCCAA
    AGTTTGAGCA AAGACGCTCC ATCCAAAGAA GTGGACCAGG TCACTAAACG CATGCTGTCA
    GCACGTCTAC TAAAGATCAA CGAGTTGAAG AACGCGTTGT CTGAGCTGAG GCTGCATGCG
    GACGAGCTGC AGCGAGAGAA CCGTTTACTG CGACAGCTGC AGCTCCGGCA AGAGAAAGCT
    TTGCACCGTT ACAATGACAC GGACAGCGAG ATCTCACAGC TGCTGGCTCG TCACAACAAT
    GAAACTCACG TGCTGCGCGA GAGATTGAGA CGCAGTCAAG AGAACCAACG CACAGCTGAG
    CGCAGCCTCA GGGAAAGAGA CGCTCAGCTC CAGCGCTGCC GCAGTCAAAT GCAGAAGCTG
    CAACAACTCG CAGATGACCA GAATCTTGGA GAGAGAGAGG AACTCTCGCG AAAACTAATT
    GGCACTCAAA GCAGATTGCA AGATAGTGAA CACAGAGTGA AGGAATTGGA GAAGAACATG
    GAGCTGAGTA GCGGGAGCTT TCAGAGGCAG CTTGCTAATG AGAGGAGGAA AACTCACGAT
    GCACAACAGG AAGTTAAAGC ACTGCAGGAG GAGGTCGAGA GACTTTGCAC TAAACTAAAG
    GAGAAAGAAA GGGAGCTGGA TGCCCGAAAC ATTTACGCTA ACAGGATGCA CAAAGCTTCT
    TCCAGGAAAG AAGCAGAGAA TGGCAGTAAA CGGAAAGGCT TTAATATAAC CAGCACTAAA
    GCAGTACAGA CAGAGGACAG AACATCCTCA TTGGATTTCC CATCGCCACC TCCAGCTCTT
    ACCAATAATC TTCCACCTGA TGACCAGACT GATGATTACC TTTCTCTGAA GGTTCAAAAG
    CCTACTGTGA ATCACAGGGT AAAAAAGCAG GATCAGCAAA GCAGAGAGCA CAGGACGAGA
    GAATCGAAGG AATGGAAGAT TCAAGAGTTT TGGAGAGAAA GGGAGAAAGA AAGACACATG
    GAATCTGCAA GAGAACAGGA GAAAGAAAAA GAGACACTGC ATGAGAGAGA AAGACAAACA
    GAGCTGCTGA AAGATGTGGA GAGGCAAAGA CTAGATCAAG ATCTCAGCTC AAATGAGAAG
    AACTTTAAAG GAAACAGAGG CCTAAACAGA GAGGAGGAAG ACAGGAGGAT TGGCATGTCT
    GTGTCTCAAA AGGAAGAAGA GATCCAAAAG CAGCGTGAAC TGGAAGAGGA GCAACAAAAA
    GTGAGAAATC GGGAGATCCA GGACTACAAT CAGGAGCGCG TGGAAGAAGA ACGTCAGCGT
    AAAGAGCAGC TATTAGCTAA GATGCGTGAG ATTGACATTC AAACTCAAGG TCAGGACTCC
    GACTTCTTTT CAGATGACAC AGGAAGTGTT CGCTCTCCTC CGCGATTGTC TGAGCAACGG
    AACCACAATG GTTTTTTCAA GTTAACAGAG CCAGATGAAA ACATAAACAC ATTTTCAAGC
    GGAAGAAGAG GAGGCAGTCT CCGAGCTCAG GCACCCAACG AGGATCTTGA TCTTGCTTTC
    GGCAGCTACG CACCTTCTTT TGGTAAGTCC ACCCCACGCG CAGGTTTGGG GACACGAAAT
    GCACAAAATC ACTCCCCTCG AGAACTTGAT GAAGGGCTGG ATCTGGGTGG CCTGGTCAAA
    GAGAGGAAGT CAAACCTCAT GCAGCAGCTT TTTGGGGCCA CTGCAACTCC ACCTCCTTCA
    GACCCATCCA GCAAGATGGA GATCCTGAGC CCTCCTCTAA CAACACAGTT TTCAGGACGC
    AGGAGGGACA CAGACACACC AAATGAACTA AACATCAGTT CTCTCCCCAA CAACAGGAGC
    ACTCTACAAG TCTCAGAGAG CCGACCCGCG GTCAGGGCCA TTACATCATT TGATGATGAT
    ATAGAGGAAC TCACACTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021325688.1
    CDS402..2702
    Translation

    Target ORF information:

    RefSeq Version XM_021470013.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Leber congenital amaurosis 5 (lca5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021470013.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGACTTGC ATGAAGATAA TCAGGAATCG GAGCACAGCC GATTGTCTCA CCATAATGAA 
    AGGAAGATCT TAGAATCCCT TCGATCTGGA AAGCAAGAGA ACAATGGAAA AGACATGGGG
    AACTCCGCTG GGGATCATGC ACAGAGCAGA ACTCGAGAGA CAGCTGGAGA TCCAGGCCGC
    AATGGGTTTT CAGATGGAGA GCGCAGCAGT GGCTCGTTCT ACTCTGATGA TTATGAAAAC
    GCCTCCCATT CAGATCGGTC ACTCTCACCG TCAGCTTCTC CATCGCCACC CAGAAGAGGG
    AGATCTAGGA GAGTTTCCAG CAGCCCTTTA CATAAGACAG GTGTGCACAA AGGTGCATCT
    CGCCGTATCC TGTCCCACTC CCATCATCCT CAGCAACGAG CAGGAGCAAT CCGATCCCAA
    AGTTTGAGCA AAGACGCTCC ATCCAAAGAA GTGGACCAGG TCACTAAACG CATGCTGTCA
    GCACGTCTAC TAAAGATCAA CGAGTTGAAG AACGCGTTGT CTGAGCTGAG GCTGCATGCG
    GACGAGCTGC AGCGAGAGAA CCGTTTACTG CGACAGCTGC AGCTCCGGCA AGAGAAAGCT
    TTGCACCGTT ACAATGACAC GGACAGCGAG ATCTCACAGC TGCTGGCTCG TCACAACAAT
    GAAACTCACG TGCTGCGCGA GAGATTGAGA CGCAGTCAAG AGAACCAACG CACAGCTGAG
    CGCAGCCTCA GGGAAAGAGA CGCTCAGCTC CAGCGCTGCC GCAGTCAAAT GCAGAAGCTG
    CAACAACTCG CAGATGACCA GAATCTTGGA GAGAGAGAGG AACTCTCGCG AAAACTAATT
    GGCACTCAAA GCAGATTGCA AGATAGTGAA CACAGAGTGA AGGAATTGGA GAAGAACATG
    GAGCTGAGTA GCGGGAGCTT TCAGAGGCAG CTTGCTAATG AGAGGAGGAA AACTCACGAT
    GCACAACAGG AAGTTAAAGC ACTGCAGGAG GAGGTCGAGA GACTTTGCAC TAAACTAAAG
    GAGAAAGAAA GGGAGCTGGA TGCCCGAAAC ATTTACGCTA ACAGGATGCA CAAAGCTTCT
    TCCAGGAAAG AAGCAGAGAA TGGCAGTAAA CGGAAAGGCT TTAATATAAC CAGCACTAAA
    GCAGTACAGA CAGAGGACAG AACATCCTCA TTGGATTTCC CATCGCCACC TCCAGCTCTT
    ACCAATAATC TTCCACCTGA TGACCAGACT GATGATTACC TTTCTCTGAA GGTTCAAAAG
    CCTACTGTGA ATCACAGGGT AAAAAAGCAG GATCAGCAAA GCAGAGAGCA CAGGACGAGA
    GAATCGAAGG AATGGAAGAT TCAAGAGTTT TGGAGAGAAA GGGAGAAAGA AAGACACATG
    GAATCTGCAA GAGAACAGGA GAAAGAAAAA GAGACACTGC ATGAGAGAGA AAGACAAACA
    GAGCTGCTGA AAGATGTGGA GAGGCAAAGA CTAGATCAAG ATCTCAGCTC AAATGAGAAG
    AACTTTAAAG GAAACAGAGG CCTAAACAGA GAGGAGGAAG ACAGGAGGAT TGGCATGTCT
    GTGTCTCAAA AGGAAGAAGA GATCCAAAAG CAGCGTGAAC TGGAAGAGGA GCAACAAAAA
    GTGAGAAATC GGGAGATCCA GGACTACAAT CAGGAGCGCG TGGAAGAAGA ACGTCAGCGT
    AAAGAGCAGC TATTAGCTAA GATGCGTGAG ATTGACATTC AAACTCAAGG TCAGGACTCC
    GACTTCTTTT CAGATGACAC AGGAAGTGTT CGCTCTCCTC CGCGATTGTC TGAGCAACGG
    AACCACAATG GTTTTTTCAA GTTAACAGAG CCAGATGAAA ACATAAACAC ATTTTCAAGC
    GGAAGAAGAG GAGGCAGTCT CCGAGCTCAG GCACCCAACG AGGATCTTGA TCTTGCTTTC
    GGCAGCTACG CACCTTCTTT TGGTAAGTCC ACCCCACGCG CAGGTTTGGG GACACGAAAT
    GCACAAAATC ACTCCCCTCG AGAACTTGAT GAAGGGCTGG ATCTGGGTGG CCTGGTCAAA
    GAGAGGAAGT CAAACCTCAT GCAGCAGCTT TTTGGGGCCA CTGCAACTCC ACCTCCTTCA
    GACCCATCCA GCAAGATGGA GATCCTGAGC CCTCCTCTAA CAACACAGTT TTCAGGACGC
    AGGAGGGACA CAGACACACC AAATGAACTA AACATCAGTT CTCTCCCCAA CAACAGGAGC
    ACTCTACAAG TCTCAGAGAG CCGACCCGCG GTCAGGGCCA TTACATCATT TGATGATGAT
    ATAGAGGAAC TCACACTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa57514
    Clone ID Related Accession (Same CDS sequence) XM_021470016.1 , XM_017353736.1
    Accession Version XM_021470016.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2262bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product lebercilin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGACTTGC ATGAAGATAA TCAGGAATCG GAGCACAGCC GATTGTCTCA CCATAATGAA 
    AGGAAGATCT TAGAATCCCT TCGATCTGGA AAGCAAGAGA ACAATGGAAA AGACATGGGG
    AACTCCGCTG GGGATCATGC ACAGAGCAGA ACTCGAGAGA CAGCTGGAGA TCCAGGCCGC
    AATGGGTTTT CAGATGGAGA GCGCAGCAGT GGCTCGTTCT ACTCTGATGA TTATGAAAAC
    GCCTCCCATT CAGATCGGTC ACTCTCACCG TCAGCTTCTC CATCGCCACC CAGAAGAGGG
    AGATCTAGGA GAGTTTCCAG CAGCCCTTTA CATAAGACAG GTGTGCACAA AGGTGCATCT
    CGCCGTATCC TGTCCCACTC CCATCATCCT CAGCAACGAG CAGGAGCAAT CCGATCCCAA
    AGTTTGAGCA AAGACGCTCC ATCCAAAGAA GTGGACCAGG TCACTAAACG CATGCTGTCA
    GCACGTCTAC TAAAGATCAA CGAGTTGAAG AACGCGTTGT CTGAGCTGAG GCTGCATGCG
    GACGAGCTGC AGCGAGAGAA CCGTTTACTG CGACAGCTGC AGCTCCGGCA AGAGAAAGCT
    TTGCACCGTT ACAATGACAC GGACAGCGAG ATCTCACAGC TGCTGGCTCG TCACAACAAT
    GAAACTCACG TGCTGCGCGA GAGATTGAGA CGCAGTCAAG AGAACCAACG CACAGCTGAG
    CGCAGCCTCA GGGAAAGAGA CGCTCAGCTC CAGCGCTGCC GCAGTCAAAT GCAGAAGCTG
    CAACAACTCG CAGATGACCA GAATCTTGGA GAGAGAGAGG AACTCTCGCG AAAACTAATT
    GGCACTCAAA GCAGATTGCA AGATAGTGAA CACAGAGTGA AGGAATTGGA GAAGAACATG
    GAGCTGAGTA GCGGGAGCTT TCAGAGGCAG CTTGCTAATG AGAGGAGGAA AACTCACGAT
    GCACAACAGG AAGTTAAAGC ACTGCAGGAG GAGGTCGAGA GACTTTGCAC TAAACTAAAG
    GAGAAAGAAA GGGAGCTGGA TGCCCGAAAC ATTTACGCTA ACAGGATGCA CAAAGCTTCT
    TCCAGGAAAG AAGCAGAGAA TGGCAGTAAA CGGAAAGGCT TTAATATAAC CAGCACTAAA
    GCAGTACAGA CAGAGGACAG AACATCCTCA TTGGATTTCC CATCGCCACC TCCAGCTCTT
    ACCAATAATC TTCCACCTGA TGACCAGACT GATGATTACC TTTCTCTGAA GGATCAGCAA
    AGCAGAGAGC ACAGGACGAG AGAATCGAAG GAATGGAAGA TTCAAGAGTT TTGGAGAGAA
    AGGGAGAAAG AAAGACACAT GGAATCTGCA AGAGAACAGG AGAAAGAAAA AGAGACACTG
    CATGAGAGAG AAAGACAAAC AGAGCTGCTG AAAGATGTGG AGAGGCAAAG ACTAGATCAA
    GATCTCAGCT CAAATGAGAA GAACTTTAAA GGAAACAGAG GCCTAAACAG AGAGGAGGAA
    GACAGGAGGA TTGGCATGTC TGTGTCTCAA AAGGAAGAAG AGATCCAAAA GCAGCGTGAA
    CTGGAAGAGG AGCAACAAAA AGTGAGAAAT CGGGAGATCC AGGACTACAA TCAGGAGCGC
    GTGGAAGAAG AACGTCAGCG TAAAGAGCAG CTATTAGCTA AGATGCGTGA GATTGACATT
    CAAACTCAAG GTCAGGACTC CGACTTCTTT TCAGATGACA CAGGAAGTGT TCGCTCTCCT
    CCGCGATTGT CTGAGCAACG GAACCACAAT GGTTTTTTCA AGTTAACAGA GCCAGATGAA
    AACATAAACA CATTTTCAAG CGGAAGAAGA GGAGGCAGTC TCCGAGCTCA GGCACCCAAC
    GAGGATCTTG ATCTTGCTTT CGGCAGCTAC GCACCTTCTT TTGGTAAGTC CACCCCACGC
    GCAGGTTTGG GGACACGAAA TGCACAAAAT CACTCCCCTC GAGAACTTGA TGAAGGGCTG
    GATCTGGGTG GCCTGGTCAA AGAGAGGAAG TCAAACCTCA TGCAGCAGCT TTTTGGGGCC
    ACTGCAACTC CACCTCCTTC AGACCCATCC AGCAAGATGG AGATCCTGAG CCCTCCTCTA
    ACAACACAGT TTTCAGGACG CAGGAGGGAC ACAGACACAC CAAATGAACT AAACATCAGT
    TCTCTCCCCA ACAACAGGAG CACTCTACAA GTCTCAGAGA GCCGACCCGC GGTCAGGGCC
    ATTACATCAT TTGATGATGA TATAGAGGAA CTCACACTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021325691.1
    CDS402..2663
    Translation

    Target ORF information:

    RefSeq Version XM_021470016.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Leber congenital amaurosis 5 (lca5), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021470016.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGACTTGC ATGAAGATAA TCAGGAATCG GAGCACAGCC GATTGTCTCA CCATAATGAA 
    AGGAAGATCT TAGAATCCCT TCGATCTGGA AAGCAAGAGA ACAATGGAAA AGACATGGGG
    AACTCCGCTG GGGATCATGC ACAGAGCAGA ACTCGAGAGA CAGCTGGAGA TCCAGGCCGC
    AATGGGTTTT CAGATGGAGA GCGCAGCAGT GGCTCGTTCT ACTCTGATGA TTATGAAAAC
    GCCTCCCATT CAGATCGGTC ACTCTCACCG TCAGCTTCTC CATCGCCACC CAGAAGAGGG
    AGATCTAGGA GAGTTTCCAG CAGCCCTTTA CATAAGACAG GTGTGCACAA AGGTGCATCT
    CGCCGTATCC TGTCCCACTC CCATCATCCT CAGCAACGAG CAGGAGCAAT CCGATCCCAA
    AGTTTGAGCA AAGACGCTCC ATCCAAAGAA GTGGACCAGG TCACTAAACG CATGCTGTCA
    GCACGTCTAC TAAAGATCAA CGAGTTGAAG AACGCGTTGT CTGAGCTGAG GCTGCATGCG
    GACGAGCTGC AGCGAGAGAA CCGTTTACTG CGACAGCTGC AGCTCCGGCA AGAGAAAGCT
    TTGCACCGTT ACAATGACAC GGACAGCGAG ATCTCACAGC TGCTGGCTCG TCACAACAAT
    GAAACTCACG TGCTGCGCGA GAGATTGAGA CGCAGTCAAG AGAACCAACG CACAGCTGAG
    CGCAGCCTCA GGGAAAGAGA CGCTCAGCTC CAGCGCTGCC GCAGTCAAAT GCAGAAGCTG
    CAACAACTCG CAGATGACCA GAATCTTGGA GAGAGAGAGG AACTCTCGCG AAAACTAATT
    GGCACTCAAA GCAGATTGCA AGATAGTGAA CACAGAGTGA AGGAATTGGA GAAGAACATG
    GAGCTGAGTA GCGGGAGCTT TCAGAGGCAG CTTGCTAATG AGAGGAGGAA AACTCACGAT
    GCACAACAGG AAGTTAAAGC ACTGCAGGAG GAGGTCGAGA GACTTTGCAC TAAACTAAAG
    GAGAAAGAAA GGGAGCTGGA TGCCCGAAAC ATTTACGCTA ACAGGATGCA CAAAGCTTCT
    TCCAGGAAAG AAGCAGAGAA TGGCAGTAAA CGGAAAGGCT TTAATATAAC CAGCACTAAA
    GCAGTACAGA CAGAGGACAG AACATCCTCA TTGGATTTCC CATCGCCACC TCCAGCTCTT
    ACCAATAATC TTCCACCTGA TGACCAGACT GATGATTACC TTTCTCTGAA GGATCAGCAA
    AGCAGAGAGC ACAGGACGAG AGAATCGAAG GAATGGAAGA TTCAAGAGTT TTGGAGAGAA
    AGGGAGAAAG AAAGACACAT GGAATCTGCA AGAGAACAGG AGAAAGAAAA AGAGACACTG
    CATGAGAGAG AAAGACAAAC AGAGCTGCTG AAAGATGTGG AGAGGCAAAG ACTAGATCAA
    GATCTCAGCT CAAATGAGAA GAACTTTAAA GGAAACAGAG GCCTAAACAG AGAGGAGGAA
    GACAGGAGGA TTGGCATGTC TGTGTCTCAA AAGGAAGAAG AGATCCAAAA GCAGCGTGAA
    CTGGAAGAGG AGCAACAAAA AGTGAGAAAT CGGGAGATCC AGGACTACAA TCAGGAGCGC
    GTGGAAGAAG AACGTCAGCG TAAAGAGCAG CTATTAGCTA AGATGCGTGA GATTGACATT
    CAAACTCAAG GTCAGGACTC CGACTTCTTT TCAGATGACA CAGGAAGTGT TCGCTCTCCT
    CCGCGATTGT CTGAGCAACG GAACCACAAT GGTTTTTTCA AGTTAACAGA GCCAGATGAA
    AACATAAACA CATTTTCAAG CGGAAGAAGA GGAGGCAGTC TCCGAGCTCA GGCACCCAAC
    GAGGATCTTG ATCTTGCTTT CGGCAGCTAC GCACCTTCTT TTGGTAAGTC CACCCCACGC
    GCAGGTTTGG GGACACGAAA TGCACAAAAT CACTCCCCTC GAGAACTTGA TGAAGGGCTG
    GATCTGGGTG GCCTGGTCAA AGAGAGGAAG TCAAACCTCA TGCAGCAGCT TTTTGGGGCC
    ACTGCAACTC CACCTCCTTC AGACCCATCC AGCAAGATGG AGATCCTGAG CCCTCCTCTA
    ACAACACAGT TTTCAGGACG CAGGAGGGAC ACAGACACAC CAAATGAACT AAACATCAGT
    TCTCTCCCCA ACAACAGGAG CACTCTACAA GTCTCAGAGA GCCGACCCGC GGTCAGGGCC
    ATTACATCAT TTGATGATGA TATAGAGGAA CTCACACTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa57514
    Clone ID Related Accession (Same CDS sequence) XM_021470016.1 , XM_017353736.1
    Accession Version XM_017353736.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2262bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product lebercilin isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGACTTGC ATGAAGATAA TCAGGAATCG GAGCACAGCC GATTGTCTCA CCATAATGAA 
    AGGAAGATCT TAGAATCCCT TCGATCTGGA AAGCAAGAGA ACAATGGAAA AGACATGGGG
    AACTCCGCTG GGGATCATGC ACAGAGCAGA ACTCGAGAGA CAGCTGGAGA TCCAGGCCGC
    AATGGGTTTT CAGATGGAGA GCGCAGCAGT GGCTCGTTCT ACTCTGATGA TTATGAAAAC
    GCCTCCCATT CAGATCGGTC ACTCTCACCG TCAGCTTCTC CATCGCCACC CAGAAGAGGG
    AGATCTAGGA GAGTTTCCAG CAGCCCTTTA CATAAGACAG GTGTGCACAA AGGTGCATCT
    CGCCGTATCC TGTCCCACTC CCATCATCCT CAGCAACGAG CAGGAGCAAT CCGATCCCAA
    AGTTTGAGCA AAGACGCTCC ATCCAAAGAA GTGGACCAGG TCACTAAACG CATGCTGTCA
    GCACGTCTAC TAAAGATCAA CGAGTTGAAG AACGCGTTGT CTGAGCTGAG GCTGCATGCG
    GACGAGCTGC AGCGAGAGAA CCGTTTACTG CGACAGCTGC AGCTCCGGCA AGAGAAAGCT
    TTGCACCGTT ACAATGACAC GGACAGCGAG ATCTCACAGC TGCTGGCTCG TCACAACAAT
    GAAACTCACG TGCTGCGCGA GAGATTGAGA CGCAGTCAAG AGAACCAACG CACAGCTGAG
    CGCAGCCTCA GGGAAAGAGA CGCTCAGCTC CAGCGCTGCC GCAGTCAAAT GCAGAAGCTG
    CAACAACTCG CAGATGACCA GAATCTTGGA GAGAGAGAGG AACTCTCGCG AAAACTAATT
    GGCACTCAAA GCAGATTGCA AGATAGTGAA CACAGAGTGA AGGAATTGGA GAAGAACATG
    GAGCTGAGTA GCGGGAGCTT TCAGAGGCAG CTTGCTAATG AGAGGAGGAA AACTCACGAT
    GCACAACAGG AAGTTAAAGC ACTGCAGGAG GAGGTCGAGA GACTTTGCAC TAAACTAAAG
    GAGAAAGAAA GGGAGCTGGA TGCCCGAAAC ATTTACGCTA ACAGGATGCA CAAAGCTTCT
    TCCAGGAAAG AAGCAGAGAA TGGCAGTAAA CGGAAAGGCT TTAATATAAC CAGCACTAAA
    GCAGTACAGA CAGAGGACAG AACATCCTCA TTGGATTTCC CATCGCCACC TCCAGCTCTT
    ACCAATAATC TTCCACCTGA TGACCAGACT GATGATTACC TTTCTCTGAA GGATCAGCAA
    AGCAGAGAGC ACAGGACGAG AGAATCGAAG GAATGGAAGA TTCAAGAGTT TTGGAGAGAA
    AGGGAGAAAG AAAGACACAT GGAATCTGCA AGAGAACAGG AGAAAGAAAA AGAGACACTG
    CATGAGAGAG AAAGACAAAC AGAGCTGCTG AAAGATGTGG AGAGGCAAAG ACTAGATCAA
    GATCTCAGCT CAAATGAGAA GAACTTTAAA GGAAACAGAG GCCTAAACAG AGAGGAGGAA
    GACAGGAGGA TTGGCATGTC TGTGTCTCAA AAGGAAGAAG AGATCCAAAA GCAGCGTGAA
    CTGGAAGAGG AGCAACAAAA AGTGAGAAAT CGGGAGATCC AGGACTACAA TCAGGAGCGC
    GTGGAAGAAG AACGTCAGCG TAAAGAGCAG CTATTAGCTA AGATGCGTGA GATTGACATT
    CAAACTCAAG GTCAGGACTC CGACTTCTTT TCAGATGACA CAGGAAGTGT TCGCTCTCCT
    CCGCGATTGT CTGAGCAACG GAACCACAAT GGTTTTTTCA AGTTAACAGA GCCAGATGAA
    AACATAAACA CATTTTCAAG CGGAAGAAGA GGAGGCAGTC TCCGAGCTCA GGCACCCAAC
    GAGGATCTTG ATCTTGCTTT CGGCAGCTAC GCACCTTCTT TTGGTAAGTC CACCCCACGC
    GCAGGTTTGG GGACACGAAA TGCACAAAAT CACTCCCCTC GAGAACTTGA TGAAGGGCTG
    GATCTGGGTG GCCTGGTCAA AGAGAGGAAG TCAAACCTCA TGCAGCAGCT TTTTGGGGCC
    ACTGCAACTC CACCTCCTTC AGACCCATCC AGCAAGATGG AGATCCTGAG CCCTCCTCTA
    ACAACACAGT TTTCAGGACG CAGGAGGGAC ACAGACACAC CAAATGAACT AAACATCAGT
    TCTCTCCCCA ACAACAGGAG CACTCTACAA GTCTCAGAGA GCCGACCCGC GGTCAGGGCC
    ATTACATCAT TTGATGATGA TATAGAGGAA CTCACACTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017209225.1
    CDS351..2612
    Translation

    Target ORF information:

    RefSeq Version XM_017353736.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Leber congenital amaurosis 5 (lca5), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017353736.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGACTTGC ATGAAGATAA TCAGGAATCG GAGCACAGCC GATTGTCTCA CCATAATGAA 
    AGGAAGATCT TAGAATCCCT TCGATCTGGA AAGCAAGAGA ACAATGGAAA AGACATGGGG
    AACTCCGCTG GGGATCATGC ACAGAGCAGA ACTCGAGAGA CAGCTGGAGA TCCAGGCCGC
    AATGGGTTTT CAGATGGAGA GCGCAGCAGT GGCTCGTTCT ACTCTGATGA TTATGAAAAC
    GCCTCCCATT CAGATCGGTC ACTCTCACCG TCAGCTTCTC CATCGCCACC CAGAAGAGGG
    AGATCTAGGA GAGTTTCCAG CAGCCCTTTA CATAAGACAG GTGTGCACAA AGGTGCATCT
    CGCCGTATCC TGTCCCACTC CCATCATCCT CAGCAACGAG CAGGAGCAAT CCGATCCCAA
    AGTTTGAGCA AAGACGCTCC ATCCAAAGAA GTGGACCAGG TCACTAAACG CATGCTGTCA
    GCACGTCTAC TAAAGATCAA CGAGTTGAAG AACGCGTTGT CTGAGCTGAG GCTGCATGCG
    GACGAGCTGC AGCGAGAGAA CCGTTTACTG CGACAGCTGC AGCTCCGGCA AGAGAAAGCT
    TTGCACCGTT ACAATGACAC GGACAGCGAG ATCTCACAGC TGCTGGCTCG TCACAACAAT
    GAAACTCACG TGCTGCGCGA GAGATTGAGA CGCAGTCAAG AGAACCAACG CACAGCTGAG
    CGCAGCCTCA GGGAAAGAGA CGCTCAGCTC CAGCGCTGCC GCAGTCAAAT GCAGAAGCTG
    CAACAACTCG CAGATGACCA GAATCTTGGA GAGAGAGAGG AACTCTCGCG AAAACTAATT
    GGCACTCAAA GCAGATTGCA AGATAGTGAA CACAGAGTGA AGGAATTGGA GAAGAACATG
    GAGCTGAGTA GCGGGAGCTT TCAGAGGCAG CTTGCTAATG AGAGGAGGAA AACTCACGAT
    GCACAACAGG AAGTTAAAGC ACTGCAGGAG GAGGTCGAGA GACTTTGCAC TAAACTAAAG
    GAGAAAGAAA GGGAGCTGGA TGCCCGAAAC ATTTACGCTA ACAGGATGCA CAAAGCTTCT
    TCCAGGAAAG AAGCAGAGAA TGGCAGTAAA CGGAAAGGCT TTAATATAAC CAGCACTAAA
    GCAGTACAGA CAGAGGACAG AACATCCTCA TTGGATTTCC CATCGCCACC TCCAGCTCTT
    ACCAATAATC TTCCACCTGA TGACCAGACT GATGATTACC TTTCTCTGAA GGATCAGCAA
    AGCAGAGAGC ACAGGACGAG AGAATCGAAG GAATGGAAGA TTCAAGAGTT TTGGAGAGAA
    AGGGAGAAAG AAAGACACAT GGAATCTGCA AGAGAACAGG AGAAAGAAAA AGAGACACTG
    CATGAGAGAG AAAGACAAAC AGAGCTGCTG AAAGATGTGG AGAGGCAAAG ACTAGATCAA
    GATCTCAGCT CAAATGAGAA GAACTTTAAA GGAAACAGAG GCCTAAACAG AGAGGAGGAA
    GACAGGAGGA TTGGCATGTC TGTGTCTCAA AAGGAAGAAG AGATCCAAAA GCAGCGTGAA
    CTGGAAGAGG AGCAACAAAA AGTGAGAAAT CGGGAGATCC AGGACTACAA TCAGGAGCGC
    GTGGAAGAAG AACGTCAGCG TAAAGAGCAG CTATTAGCTA AGATGCGTGA GATTGACATT
    CAAACTCAAG GTCAGGACTC CGACTTCTTT TCAGATGACA CAGGAAGTGT TCGCTCTCCT
    CCGCGATTGT CTGAGCAACG GAACCACAAT GGTTTTTTCA AGTTAACAGA GCCAGATGAA
    AACATAAACA CATTTTCAAG CGGAAGAAGA GGAGGCAGTC TCCGAGCTCA GGCACCCAAC
    GAGGATCTTG ATCTTGCTTT CGGCAGCTAC GCACCTTCTT TTGGTAAGTC CACCCCACGC
    GCAGGTTTGG GGACACGAAA TGCACAAAAT CACTCCCCTC GAGAACTTGA TGAAGGGCTG
    GATCTGGGTG GCCTGGTCAA AGAGAGGAAG TCAAACCTCA TGCAGCAGCT TTTTGGGGCC
    ACTGCAACTC CACCTCCTTC AGACCCATCC AGCAAGATGG AGATCCTGAG CCCTCCTCTA
    ACAACACAGT TTTCAGGACG CAGGAGGGAC ACAGACACAC CAAATGAACT AAACATCAGT
    TCTCTCCCCA ACAACAGGAG CACTCTACAA GTCTCAGAGA GCCGACCCGC GGTCAGGGCC
    ATTACATCAT TTGATGATGA TATAGAGGAA CTCACACTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.