madd cDNA ORF clone, Danio rerio(zebrafish)

The following madd gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the madd cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa143465 XM_021477876.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa143466 XM_021477877.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa143467 XM_021477878.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa143468 XM_021477879.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa143469 XM_021477880.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa143470 XM_021477881.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa143471 XM_021477882.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa143472 XM_021477883.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa143473 XM_021477884.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa143474 XM_021477885.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa143475 XM_021477886.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa143476 XM_021477887.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa143477 XM_021477888.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa143478 XM_021477889.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa143479 XM_021477890.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa143480 XM_021477891.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa143481 XM_021477892.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa143482 XM_021477894.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X25, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa143483 XM_021477895.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X26, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa143484 XM_021477896.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X27, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa143485 XM_021477897.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X28, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa143486 XM_021477899.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X29, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa143487 XM_021477900.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X30, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa143489 XM_021477906.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X32, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa144881 XM_021471950.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X40, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa144883 XM_021471953.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X42, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa144884 XM_021471954.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X43, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa144885 XM_021471955.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X44, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa144886 XM_021471956.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X45, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa144888 XM_021471958.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X47, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa144889 XM_021471959.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X48, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa144891 XM_021471961.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X50, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa144894 XM_021471964.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X53, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa144895 XM_021471965.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X54, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa144896 XM_021471966.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X55, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa144897 XM_021471968.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X56, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa144898 XM_021471969.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X57, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa144899 XM_021471970.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X58, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa144900 XM_021471971.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X59, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa144902 XM_021471973.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X61, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa144904 XM_021471975.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X63, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa144905 XM_021471976.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa144906 XM_021471977.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X64, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa144907 XM_021471978.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X65, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa144908 XM_021471979.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X66, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa144911 XM_021471982.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa144912 XM_021471984.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa144914 XM_021471986.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X68, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa144915 XM_021471987.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X69, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa144916 XM_021471988.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X70, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa144917 XM_021471989.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X71, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa144918 XM_021471990.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X72, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa144919 XM_021471991.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X73, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa59767 XM_017357062.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa13173 XM_021477902.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa13173 XM_009303285.2
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa13174 XM_009303290.2
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X27, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa13174 XM_021477904.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa13175 XM_021477905.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa13175 XM_009303291.2
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X28, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa13176 XM_009303298.3
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X39, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa48914 XM_009303265.2
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa48915 XM_009303266.2
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa48916 XM_009303267.2
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa48917 XM_009303268.2
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa48918 XM_009303269.2
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa48919 XM_009303270.2
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa48920 XM_009303271.2
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa48921 XM_009303272.2
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa48922 XM_009303273.2
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa48923 XM_009303274.2
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa48924 XM_009303275.2
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa48925 XM_009303276.3
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa48926 XM_009303277.2
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa48927 XM_009303278.3
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa48928 XM_009303279.2
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa48929 XM_009303280.2
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa48930 XM_017357063.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa48931 XM_009303282.2
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa48932 XM_021477898.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa48932 XM_009303283.2
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa48933 XM_009303284.2
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa48934 XM_009303286.2
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa48935 XM_009303287.2
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa48936 XM_009303288.2
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X25, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa48937 XM_009303289.2
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X26, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa48937 XM_021477903.1
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa48938 XM_009303292.2
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X29, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa48939 XM_009303293.2
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X30, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa48940 XM_009303294.3
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X35, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa48941 XM_009303295.3
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X36, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa48942 XM_009303296.3
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X37, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa48943 XM_009303297.3
Latest version!
Danio rerio MAP-kinase activating death domain (madd), transcript variant X38, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
View Old Accession Versions >>
ODa48941 XM_009303295.2 Danio rerio MAP-kinase activating death domain (madd), transcript variant X32, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa48940 XM_009303294.2 Danio rerio MAP-kinase activating death domain (madd), transcript variant X31, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa13176 XM_009303298.2 Danio rerio MAP-kinase activating death domain (madd), transcript variant X35, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa48927 XM_009303278.2 Danio rerio MAP-kinase activating death domain (madd), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa48942 XM_009303296.2 Danio rerio MAP-kinase activating death domain (madd), transcript variant X33, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa48943 XM_009303297.2 Danio rerio MAP-kinase activating death domain (madd), transcript variant X34, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa48925 XM_009303276.2 Danio rerio MAP-kinase activating death domain (madd), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa143465
    Clone ID Related Accession (Same CDS sequence) XM_021477876.1
    Accession Version XM_021477876.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5154bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA TTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGAACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCAGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT ACCCAAGCTC TGCCCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTTCCCAGC CTCAAAGAAT ATTTTAATTT TGATTTAGCA
    GAGGACACAG ATCAAGACGA TGCTGATGAC AGTCCTGAAA GTCCTGTATT TGGGCTAAAT
    TCTCTAATGG AGATTGTAAC AGAGATCGGC CCGGGGAGCG GAGAAGGTGC CCGAGCCCCC
    AGAGCACTGG TAGATCAGAA ATCCTCTGTT ATTAAGCACA GCCCTACTGT GAAGAGAGAG
    TCTCCGTCTC CTCAAGGCAG AGCCAACAAT ACCAGTGAGA ACCAGCAGTT TCTAAAGGAG
    GTGGTCCAGA GCGTGCTGGA GGGGCAGGGT GTGGGCTGGC TTAACATGAA GAAGGTGCGT
    CGCCTGCTAG AGAATGAACA GCTCAGAGTC TTTGTGCTCA GCAAACTCAA CCGTGCGGTC
    CAGTCAGAGG AGGACGCCCA GCAGGAAGTT ATAAGAGATG TGGAGATAAA TAGAAAGGTC
    TACAAAGGCA TGCTGGATAT TTTGAAGTGC ACGGTGTCCA GTTTGGAGCA TTCATACACT
    AATGCTGGCT TAGGGGGCAT GGCCAGTGTC TTCAGTCTGC TAGAGATAGC ACGCACCCAC
    TACCAAACCA AAGATCCAGA GAAGCGGAAA CGGAGCCCCA CCGAAGGGCT TAGCAGTCCA
    GGCAGTAAGG AGAGCCCATC GGGCCGAATG GAGAGTGCCA GGGCGGCAGG GGTGCTTCTG
    GTACCCCGTA TCCAATTGCC GCCACCCTCC TCTGGGAAAT CATCACGCCA GTTTGATACC
    CGGAGTCTGA ACGAGGAGAA TTTTATTGCC TCCATTGAAT TGTGGAGCAA GCACCAGGAT
    AACAGAAAGC AAAAAGCTTT GGAAAAAGAA CAGAGTGGTT TAAATAAACG GAAATCAGGA
    GCGGATGGAG TAAAACAACG TGTAGAAGGA GGAGACACTG AGGAGAAGAA ATCACAGATC
    AGTGCAGACA GTGGCCTCAG TGTGACATCT GGCTCACAGA AGAGTGATAC GGATTCCCTG
    GCGAGCTCTG AACCTCCTGC TCTGACCAGA AGCACCAGCC AGGACTCAGA GGCCAGCACA
    GTGGTAAGTA ACAGCTCAGG TGAGACGCTC GGTGCAGACA GTGACCTGAG CAGCACTGCT
    GGAGATGGCC TGACAGGAAG ACATGGTCAG CATCTCAACC TGTCCCGTGG CACACTGTCT
    GACAGTGAGA TTGAGACCAA CCCTGCAACC AGCTCAGTGT TTGGCAAGAC ACACAAGCTG
    AAGCCAGGAG TGAAGGAGCC TGTTGGTGTT AATAAAGCAG CACCTGCTCC ACCCGTTGAG
    GATGTTAGCA TGAGGATCTA TCTGTGTGAG GGGCTTCTGG GCCGGGATAA AAGCTCAGTT
    TGGGACCAGT TGGAGGATGC TGCAATGGAA ACCTTTTCTC TGAGTAAAGA GCGCTCAACT
    CTGTGGGATC AGATGCAGTT CTGGGAGGAC GCCTTCCTGG ATGCCGTAAT GCTGGAACGG
    GAAGGAATGG GAATGGATCA GGGACCACAA GAAATGATTG ACAGGTATCT TTCTCTGGGA
    GATCATGACC GCAAGAGACT GGAGGATGAT GAGGACAGAC TGCTGGCCAC GCTTCTGCAC
    AATATGATTG CTTACATGCT TATGATGAAG GTCGGTAAAA ACGACATCAG GAAGAAAGTA
    CGTCGTCTTA TGGGCAAATC CCACATTGGC CTCACTCACA GTCAGGAGAT CAATGAAGTC
    CTGGATCGCC TCGCACATTT AAGCGGTCGG GAGCTTTCTA TCAGGCCGAG TGGCAGCAGG
    CATATTAAAA AACAGACATT TGTTGTGCAT GCTGGGACAG ATACAACTGG TGACATATTC
    TTCATGGAGG TGTGTGATGA TTGCATCGTC TTGCGCAGTA ATATTGGCAC AGTCTATGAG
    CGCTGGTGGT ATGAGAAGCT CATAAATATG ACTTACTGTC CCAAAACTAA AGTGTTGTGT
    CTGTGGAGGA GAAACGGCCA GGAGACGCAG CTAAACAAGT TTTACACTAA GAAGTGTCGG
    GAATTGTACT ACTGTGTTAA AGACAGCATG GAGCGTGCTG CTGCTCGACA ACAAAGCATC
    AAACCAGGTC CAGAGCTTGG AGGCGAGTTT CCGGTTCAGG ACATGAAGAC AGGGGAGGGT
    GGTCTCCTCC AAGTCACACT GGAAGGCATC AACCTCAAAT TTATGCACAG CCAGGAGCGG
    AAGGTTTTCA TAGAGCTGAA TCACATTAAA AAGTGCAATA CAGTGAAAGG AGTCTTTGTC
    CTGGAGGAGT TTGTTCCTGA AACTAAAGAA GTGGTGATCC ATAAGTACAA GACTCCTATG
    GCACATCAGA TCTGTTACTC GGTGCTCTGC CTCTTCTCCT ACATGGCGGC GGTCAAGGGG
    AAAGAGTCAG AGGGCAAACC CAAGATGCTG TCCCCTCGTC CACTAGCGAG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333551.1
    CDS432..5585
    Translation

    Target ORF information:

    RefSeq Version XM_021477876.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021477876.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA TTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGAACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCAGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT ACCCAAGCTC TGCCCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTTCCCAGC CTCAAAGAAT ATTTTAATTT TGATTTAGCA
    GAGGACACAG ATCAAGACGA TGCTGATGAC AGTCCTGAAA GTCCTGTATT TGGGCTAAAT
    TCTCTAATGG AGATTGTAAC AGAGATCGGC CCGGGGAGCG GAGAAGGTGC CCGAGCCCCC
    AGAGCACTGG TAGATCAGAA ATCCTCTGTT ATTAAGCACA GCCCTACTGT GAAGAGAGAG
    TCTCCGTCTC CTCAAGGCAG AGCCAACAAT ACCAGTGAGA ACCAGCAGTT TCTAAAGGAG
    GTGGTCCAGA GCGTGCTGGA GGGGCAGGGT GTGGGCTGGC TTAACATGAA GAAGGTGCGT
    CGCCTGCTAG AGAATGAACA GCTCAGAGTC TTTGTGCTCA GCAAACTCAA CCGTGCGGTC
    CAGTCAGAGG AGGACGCCCA GCAGGAAGTT ATAAGAGATG TGGAGATAAA TAGAAAGGTC
    TACAAAGGCA TGCTGGATAT TTTGAAGTGC ACGGTGTCCA GTTTGGAGCA TTCATACACT
    AATGCTGGCT TAGGGGGCAT GGCCAGTGTC TTCAGTCTGC TAGAGATAGC ACGCACCCAC
    TACCAAACCA AAGATCCAGA GAAGCGGAAA CGGAGCCCCA CCGAAGGGCT TAGCAGTCCA
    GGCAGTAAGG AGAGCCCATC GGGCCGAATG GAGAGTGCCA GGGCGGCAGG GGTGCTTCTG
    GTACCCCGTA TCCAATTGCC GCCACCCTCC TCTGGGAAAT CATCACGCCA GTTTGATACC
    CGGAGTCTGA ACGAGGAGAA TTTTATTGCC TCCATTGAAT TGTGGAGCAA GCACCAGGAT
    AACAGAAAGC AAAAAGCTTT GGAAAAAGAA CAGAGTGGTT TAAATAAACG GAAATCAGGA
    GCGGATGGAG TAAAACAACG TGTAGAAGGA GGAGACACTG AGGAGAAGAA ATCACAGATC
    AGTGCAGACA GTGGCCTCAG TGTGACATCT GGCTCACAGA AGAGTGATAC GGATTCCCTG
    GCGAGCTCTG AACCTCCTGC TCTGACCAGA AGCACCAGCC AGGACTCAGA GGCCAGCACA
    GTGGTAAGTA ACAGCTCAGG TGAGACGCTC GGTGCAGACA GTGACCTGAG CAGCACTGCT
    GGAGATGGCC TGACAGGAAG ACATGGTCAG CATCTCAACC TGTCCCGTGG CACACTGTCT
    GACAGTGAGA TTGAGACCAA CCCTGCAACC AGCTCAGTGT TTGGCAAGAC ACACAAGCTG
    AAGCCAGGAG TGAAGGAGCC TGTTGGTGTT AATAAAGCAG CACCTGCTCC ACCCGTTGAG
    GATGTTAGCA TGAGGATCTA TCTGTGTGAG GGGCTTCTGG GCCGGGATAA AAGCTCAGTT
    TGGGACCAGT TGGAGGATGC TGCAATGGAA ACCTTTTCTC TGAGTAAAGA GCGCTCAACT
    CTGTGGGATC AGATGCAGTT CTGGGAGGAC GCCTTCCTGG ATGCCGTAAT GCTGGAACGG
    GAAGGAATGG GAATGGATCA GGGACCACAA GAAATGATTG ACAGGTATCT TTCTCTGGGA
    GATCATGACC GCAAGAGACT GGAGGATGAT GAGGACAGAC TGCTGGCCAC GCTTCTGCAC
    AATATGATTG CTTACATGCT TATGATGAAG GTCGGTAAAA ACGACATCAG GAAGAAAGTA
    CGTCGTCTTA TGGGCAAATC CCACATTGGC CTCACTCACA GTCAGGAGAT CAATGAAGTC
    CTGGATCGCC TCGCACATTT AAGCGGTCGG GAGCTTTCTA TCAGGCCGAG TGGCAGCAGG
    CATATTAAAA AACAGACATT TGTTGTGCAT GCTGGGACAG ATACAACTGG TGACATATTC
    TTCATGGAGG TGTGTGATGA TTGCATCGTC TTGCGCAGTA ATATTGGCAC AGTCTATGAG
    CGCTGGTGGT ATGAGAAGCT CATAAATATG ACTTACTGTC CCAAAACTAA AGTGTTGTGT
    CTGTGGAGGA GAAACGGCCA GGAGACGCAG CTAAACAAGT TTTACACTAA GAAGTGTCGG
    GAATTGTACT ACTGTGTTAA AGACAGCATG GAGCGTGCTG CTGCTCGACA ACAAAGCATC
    AAACCAGGTC CAGAGCTTGG AGGCGAGTTT CCGGTTCAGG ACATGAAGAC AGGGGAGGGT
    GGTCTCCTCC AAGTCACACT GGAAGGCATC AACCTCAAAT TTATGCACAG CCAGGAGCGG
    AAGGTTTTCA TAGAGCTGAA TCACATTAAA AAGTGCAATA CAGTGAAAGG AGTCTTTGTC
    CTGGAGGAGT TTGTTCCTGA AACTAAAGAA GTGGTGATCC ATAAGTACAA GACTCCTATG
    GCACATCAGA TCTGTTACTC GGTGCTCTGC CTCTTCTCCT ACATGGCGGC GGTCAAGGGG
    AAAGAGTCAG AGGGCAAACC CAAGATGCTG TCCCCTCGTC CACTAGCGAG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa143466
    Clone ID Related Accession (Same CDS sequence) XM_021477877.1
    Accession Version XM_021477877.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5151bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA TTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGAACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCAGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT ACCCAAGCTC TGCCCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTTCCCAGC CTCAAAGAAT ATTTTAATTT TGATTTAGCA
    GAGGACACAG ATCAAGACGA TGCTGATGAC AGTCCTGAAA GTCCTGTATT TGGGCTAAAT
    TCTCTAATGG AGATTGTAAC AGAGATCGGC CCGGGGAGCG GAGAAGGTGC CCGAGCCCCC
    AGAGCACTGG TAGATCAGAA ATCCTCTGTT ATTAAGCACA GCCCTACTGT GAAGAGAGAG
    TCTCCGTCTC CTCAAGGCAG AGCCAACAAT ACCAGTGAGA ACCAGCAGTT TCTAAAGGAG
    GTGGTCCAGA GCGTGCTGGA GGGGCAGGGT GTGGGCTGGC TTAACATGAA GAAGGTGCGT
    CGCCTGCTAG AGAATGAACA GCTCAGAGTC TTTGTGCTCA GCAAACTCAA CCGTGCGGTC
    CAGTCAGAGG AGGACGCCCA GCAGGAAGTT ATAAGAGATG TGGAGATAAA TAGAAAGGTC
    TACAAAGGCA TGCTGGATAT TTTGAAGTGC ACGGTGTCCA GTTTGGAGCA TTCATACACT
    AATGCTGGCT TAGGGGGCAT GGCCAGTGTC TTCAGTCTGC TAGAGATAGC ACGCACCCAC
    TACCAAACCA AAGATCCAGA GAAGCGGAAA CGGAGCCCCA CCGAAGGGCT TAGCAGTCCA
    GGCAGTAAGG AGAGCCCATC GGGCCGAATG GAGAGTGCCA GGGCGGCAGG GGTGCTTCTG
    GTACCCCGTA TCCAATTGCC GCCACCCTCC TCTGGGAAAT CATCACGCCA GTTTGATACC
    CGGAGTCTGA ACGAGGAGAA TTTTATTGCC TCCATTGAAT TGTGGAGCAA GCACCAGGAT
    AACAGAAAGC AAAAAGCTTT GGAAAAAGAA CAGAGTGGTT TAAATAAACG GAAATCAGGA
    GCGGATGGAG TAAAACAACG TGTAGAAGGA GGAGACACTG AGGAGAAGAA ATCACAGATC
    AGTGCAGACA GTGGCCTCAG TGTGACATCT GGCTCACAGA AGAGTGATAC GGATTCCCTG
    GCGAGCTCTG AACCTCCTGC TCTGACCAGA AGCACCAGCC AGGACTCAGA GGCCAGCACA
    GTAAGTAACA GCTCAGGTGA GACGCTCGGT GCAGACAGTG ACCTGAGCAG CACTGCTGGA
    GATGGCCTGA CAGGAAGACA TGGTCAGCAT CTCAACCTGT CCCGTGGCAC ACTGTCTGAC
    AGTGAGATTG AGACCAACCC TGCAACCAGC TCAGTGTTTG GCAAGACACA CAAGCTGAAG
    CCAGGAGTGA AGGAGCCTGT TGGTGTTAAT AAAGCAGCAC CTGCTCCACC CGTTGAGGAT
    GTTAGCATGA GGATCTATCT GTGTGAGGGG CTTCTGGGCC GGGATAAAAG CTCAGTTTGG
    GACCAGTTGG AGGATGCTGC AATGGAAACC TTTTCTCTGA GTAAAGAGCG CTCAACTCTG
    TGGGATCAGA TGCAGTTCTG GGAGGACGCC TTCCTGGATG CCGTAATGCT GGAACGGGAA
    GGAATGGGAA TGGATCAGGG ACCACAAGAA ATGATTGACA GGTATCTTTC TCTGGGAGAT
    CATGACCGCA AGAGACTGGA GGATGATGAG GACAGACTGC TGGCCACGCT TCTGCACAAT
    ATGATTGCTT ACATGCTTAT GATGAAGGTC GGTAAAAACG ACATCAGGAA GAAAGTACGT
    CGTCTTATGG GCAAATCCCA CATTGGCCTC ACTCACAGTC AGGAGATCAA TGAAGTCCTG
    GATCGCCTCG CACATTTAAG CGGTCGGGAG CTTTCTATCA GGCCGAGTGG CAGCAGGCAT
    ATTAAAAAAC AGACATTTGT TGTGCATGCT GGGACAGATA CAACTGGTGA CATATTCTTC
    ATGGAGGTGT GTGATGATTG CATCGTCTTG CGCAGTAATA TTGGCACAGT CTATGAGCGC
    TGGTGGTATG AGAAGCTCAT AAATATGACT TACTGTCCCA AAACTAAAGT GTTGTGTCTG
    TGGAGGAGAA ACGGCCAGGA GACGCAGCTA AACAAGTTTT ACACTAAGAA GTGTCGGGAA
    TTGTACTACT GTGTTAAAGA CAGCATGGAG CGTGCTGCTG CTCGACAACA AAGCATCAAA
    CCAGGTCCAG AGCTTGGAGG CGAGTTTCCG GTTCAGGACA TGAAGACAGG GGAGGGTGGT
    CTCCTCCAAG TCACACTGGA AGGCATCAAC CTCAAATTTA TGCACAGCCA GGAGCGGAAG
    GTTTTCATAG AGCTGAATCA CATTAAAAAG TGCAATACAG TGAAAGGAGT CTTTGTCCTG
    GAGGAGTTTG TTCCTGAAAC TAAAGAAGTG GTGATCCATA AGTACAAGAC TCCTATGGCA
    CATCAGATCT GTTACTCGGT GCTCTGCCTC TTCTCCTACA TGGCGGCGGT CAAGGGGAAA
    GAGTCAGAGG GCAAACCCAA GATGCTGTCC CCTCGTCCAC TAGCGAGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333552.1
    CDS432..5582
    Translation

    Target ORF information:

    RefSeq Version XM_021477877.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021477877.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA TTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGAACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCAGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT ACCCAAGCTC TGCCCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTTCCCAGC CTCAAAGAAT ATTTTAATTT TGATTTAGCA
    GAGGACACAG ATCAAGACGA TGCTGATGAC AGTCCTGAAA GTCCTGTATT TGGGCTAAAT
    TCTCTAATGG AGATTGTAAC AGAGATCGGC CCGGGGAGCG GAGAAGGTGC CCGAGCCCCC
    AGAGCACTGG TAGATCAGAA ATCCTCTGTT ATTAAGCACA GCCCTACTGT GAAGAGAGAG
    TCTCCGTCTC CTCAAGGCAG AGCCAACAAT ACCAGTGAGA ACCAGCAGTT TCTAAAGGAG
    GTGGTCCAGA GCGTGCTGGA GGGGCAGGGT GTGGGCTGGC TTAACATGAA GAAGGTGCGT
    CGCCTGCTAG AGAATGAACA GCTCAGAGTC TTTGTGCTCA GCAAACTCAA CCGTGCGGTC
    CAGTCAGAGG AGGACGCCCA GCAGGAAGTT ATAAGAGATG TGGAGATAAA TAGAAAGGTC
    TACAAAGGCA TGCTGGATAT TTTGAAGTGC ACGGTGTCCA GTTTGGAGCA TTCATACACT
    AATGCTGGCT TAGGGGGCAT GGCCAGTGTC TTCAGTCTGC TAGAGATAGC ACGCACCCAC
    TACCAAACCA AAGATCCAGA GAAGCGGAAA CGGAGCCCCA CCGAAGGGCT TAGCAGTCCA
    GGCAGTAAGG AGAGCCCATC GGGCCGAATG GAGAGTGCCA GGGCGGCAGG GGTGCTTCTG
    GTACCCCGTA TCCAATTGCC GCCACCCTCC TCTGGGAAAT CATCACGCCA GTTTGATACC
    CGGAGTCTGA ACGAGGAGAA TTTTATTGCC TCCATTGAAT TGTGGAGCAA GCACCAGGAT
    AACAGAAAGC AAAAAGCTTT GGAAAAAGAA CAGAGTGGTT TAAATAAACG GAAATCAGGA
    GCGGATGGAG TAAAACAACG TGTAGAAGGA GGAGACACTG AGGAGAAGAA ATCACAGATC
    AGTGCAGACA GTGGCCTCAG TGTGACATCT GGCTCACAGA AGAGTGATAC GGATTCCCTG
    GCGAGCTCTG AACCTCCTGC TCTGACCAGA AGCACCAGCC AGGACTCAGA GGCCAGCACA
    GTAAGTAACA GCTCAGGTGA GACGCTCGGT GCAGACAGTG ACCTGAGCAG CACTGCTGGA
    GATGGCCTGA CAGGAAGACA TGGTCAGCAT CTCAACCTGT CCCGTGGCAC ACTGTCTGAC
    AGTGAGATTG AGACCAACCC TGCAACCAGC TCAGTGTTTG GCAAGACACA CAAGCTGAAG
    CCAGGAGTGA AGGAGCCTGT TGGTGTTAAT AAAGCAGCAC CTGCTCCACC CGTTGAGGAT
    GTTAGCATGA GGATCTATCT GTGTGAGGGG CTTCTGGGCC GGGATAAAAG CTCAGTTTGG
    GACCAGTTGG AGGATGCTGC AATGGAAACC TTTTCTCTGA GTAAAGAGCG CTCAACTCTG
    TGGGATCAGA TGCAGTTCTG GGAGGACGCC TTCCTGGATG CCGTAATGCT GGAACGGGAA
    GGAATGGGAA TGGATCAGGG ACCACAAGAA ATGATTGACA GGTATCTTTC TCTGGGAGAT
    CATGACCGCA AGAGACTGGA GGATGATGAG GACAGACTGC TGGCCACGCT TCTGCACAAT
    ATGATTGCTT ACATGCTTAT GATGAAGGTC GGTAAAAACG ACATCAGGAA GAAAGTACGT
    CGTCTTATGG GCAAATCCCA CATTGGCCTC ACTCACAGTC AGGAGATCAA TGAAGTCCTG
    GATCGCCTCG CACATTTAAG CGGTCGGGAG CTTTCTATCA GGCCGAGTGG CAGCAGGCAT
    ATTAAAAAAC AGACATTTGT TGTGCATGCT GGGACAGATA CAACTGGTGA CATATTCTTC
    ATGGAGGTGT GTGATGATTG CATCGTCTTG CGCAGTAATA TTGGCACAGT CTATGAGCGC
    TGGTGGTATG AGAAGCTCAT AAATATGACT TACTGTCCCA AAACTAAAGT GTTGTGTCTG
    TGGAGGAGAA ACGGCCAGGA GACGCAGCTA AACAAGTTTT ACACTAAGAA GTGTCGGGAA
    TTGTACTACT GTGTTAAAGA CAGCATGGAG CGTGCTGCTG CTCGACAACA AAGCATCAAA
    CCAGGTCCAG AGCTTGGAGG CGAGTTTCCG GTTCAGGACA TGAAGACAGG GGAGGGTGGT
    CTCCTCCAAG TCACACTGGA AGGCATCAAC CTCAAATTTA TGCACAGCCA GGAGCGGAAG
    GTTTTCATAG AGCTGAATCA CATTAAAAAG TGCAATACAG TGAAAGGAGT CTTTGTCCTG
    GAGGAGTTTG TTCCTGAAAC TAAAGAAGTG GTGATCCATA AGTACAAGAC TCCTATGGCA
    CATCAGATCT GTTACTCGGT GCTCTGCCTC TTCTCCTACA TGGCGGCGGT CAAGGGGAAA
    GAGTCAGAGG GCAAACCCAA GATGCTGTCC CCTCGTCCAC TAGCGAGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa143467
    Clone ID Related Accession (Same CDS sequence) XM_021477878.1
    Accession Version XM_021477878.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5145bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA TTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGAACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCAGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT ACCCAAGCTC TGCCCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTTCCCAGC CTCAAAGAAT ATTTTAATTT TGATTTAGCA
    GAGGACACAG ATCAAGACGA TGCTGATGAC AGTCCTGAAA GTCCTGTATT TGGGCTAAAT
    TCTCTAATGG AGATTGTAAC AGAGATCGGC CCGGGGAGCG GAGAAGGTGC CCGAGCCCCC
    AGAGCACTGG TAGATCAGAA ATCCTCTGTT ATTAAGCACA GCCCTACTGT GAAGAGAGAG
    TCTCCGTCTC CTCAAGGCAG AGCCAACAAT ACCAGTGAGA ACCAGCAGTT TCTAAAGGAG
    GTGGTCCAGA GCGTGCTGGA GGGGCAGGGT GTGGGCTGGC TTAACATGAA GAAGGTGCGT
    CGCCTGCTAG AGAATGAACA GCTCAGAGTC TTTGTGCTCA GCAAACTCAA CCGTGCGGTC
    CAGTCAGAGG AGGACGCCCA GCAGGAAGTT ATAAGAGATG TGGAGATAAA TAGAAAGGTC
    TACAAAGGCA TGCTGGATAT TTTGAAGTGC ACGGTGTCCA GTTTGGAGCA TTCATACACT
    AATGCTGGCT TAGGGGGCAT GGCCAGTGTC TTCAGTCTGC TAGAGATAGC ACGCACCCAC
    TACCAAACCA AAGATCCAGA GAAGCGGAAA CGGAGCCCCA CCGAAGGGCT TAGCAGTCCA
    GGCAGTAAGG AGAGCCCATC GGGCCGAATG GAGAGTGCCA GGGCGGCAGG GGTGCTTCTG
    GTACCCCGTA TCCAATTGCC GCCACCCTCC TCTGGGAAAT CATCACGCCA GTTTGATACC
    CGGAGTCTGA ACGAGGAGAA TTTTATTGCC TCCATTGAAT TGTGGAGCAA GCACCAGGAT
    AACAGAAAGC AAAAAGCTTT GGAAAAAGAA CAGAGTGGTT TAAATAAACG GAAATCAGGA
    GCGGATGGAG TAAAACAACG TGTAGAAGGA GGAGACACTG AGGAGAAGAA ATCACAGATC
    AGTGCAGACA GTGGCCTCAG TGTGACATCT GGCTCACAGA AGAGTGATAC GGATTCCCTG
    GCGAGCTCTG AACCTCCTGC TCTGACCAGA AGCACCAGCC AGGACTCAGA GGCCAGCACA
    GTGGTAAGTA ACAGCTCAGG TGAGACGCTC GGTGCAGACA GTGACCTGAG CAGCACTGCT
    GGAGATGGCC TGACAGGAAG ACATGGTCAG CATCTCAACC TGTCCCGTGG CACACTGTCT
    GACAGTGAGA TTGAGACCAA CCCTGCAACC AGCTCAGTGT TTGGCAAGAC ACACAAGCTG
    AAGCCAGGAG TGAAGGAGCC TGTTGGTGTT AATAAAGCAG CACCTGCTCC ACCCGTTGAG
    GATGTTAGCA TGAGGATCTA TCTGTGTGAG GGGCTTCTGG GCCGGGATAA AAGCTCAGTT
    TGGGACCAGT TGGAGGATGC TGCAATGGAA ACCTTTTCTC TGAGTAAAGA GCGCTCAACT
    CTGTGGGATC AGATGCAGTT CTGGGAGGAC GCCTTCCTGG ATGCCGTAAT GCTGGAACGG
    GAAGGAATGG GAATGGATCA GGGACCACAA GAAATGATTG ACAGGTATCT TTCTCTGGGA
    GATCATGACC GCAAGAGACT GGAGGATGAT GAGGACAGAC TGCTGGCCAC GCTTCTGCAC
    AATATGATTG CTTACATGCT TATGATGAAG GTCGGTAAAA ACGACATCAG GAAGAAAGTA
    CGTCGTCTTA TGGGCAAATC CCACATTGGC CTCACTCACA GTCAGGAGAT CAATGAAGTC
    CTGGATCGCC TCGCACATTT AAGCGGTCGG GAGCTTTCTA TCAGGCCGAG TGGCAGCAGG
    CATATTAAAA AACAGACATT TGTTGTGCAT GCTGGGACAG ATACAACTGG TGACATATTC
    TTCATGGAGG TGTGTGATGA TTGCATCGTC TTGCGCAGTA ATATTGGCAC AGTCTATGAG
    CGCTGGTGGT ATGAGAAGCT CATAAATATG ACTTACTGTC CCAAAACTAA AGTGTTGTGT
    CTGTGGAGGA GAAACGGCCA GGAGACGCAG CTAAACAAGT TTTACACTAA GAAGTGTCGG
    GAATTGTACT ACTGTGTTAA AGACAGCATG GAGCGTGCTG CTGCTCGACA ACAAAGCATC
    AAACCAGGTC CAGAGCTTGG AGGCGAGTTT CCGGTTCAGG ACATGAAGAC AGGGGAGGGT
    GGTCTCCTCC AAGTCACACT GGAAGGCATC AACCTCAAAT TTATGCACAG CCAGGTTTTC
    ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTGAAAG GAGTCTTTGT CCTGGAGGAG
    TTTGTTCCTG AAACTAAAGA AGTGGTGATC CATAAGTACA AGACTCCTAT GGCACATCAG
    ATCTGTTACT CGGTGCTCTG CCTCTTCTCC TACATGGCGG CGGTCAAGGG GAAAGAGTCA
    GAGGGCAAAC CCAAGATGCT GTCCCCTCGT CCACTAGCGA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333553.1
    CDS432..5576
    Translation

    Target ORF information:

    RefSeq Version XM_021477878.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021477878.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA TTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGAACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCAGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT ACCCAAGCTC TGCCCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTTCCCAGC CTCAAAGAAT ATTTTAATTT TGATTTAGCA
    GAGGACACAG ATCAAGACGA TGCTGATGAC AGTCCTGAAA GTCCTGTATT TGGGCTAAAT
    TCTCTAATGG AGATTGTAAC AGAGATCGGC CCGGGGAGCG GAGAAGGTGC CCGAGCCCCC
    AGAGCACTGG TAGATCAGAA ATCCTCTGTT ATTAAGCACA GCCCTACTGT GAAGAGAGAG
    TCTCCGTCTC CTCAAGGCAG AGCCAACAAT ACCAGTGAGA ACCAGCAGTT TCTAAAGGAG
    GTGGTCCAGA GCGTGCTGGA GGGGCAGGGT GTGGGCTGGC TTAACATGAA GAAGGTGCGT
    CGCCTGCTAG AGAATGAACA GCTCAGAGTC TTTGTGCTCA GCAAACTCAA CCGTGCGGTC
    CAGTCAGAGG AGGACGCCCA GCAGGAAGTT ATAAGAGATG TGGAGATAAA TAGAAAGGTC
    TACAAAGGCA TGCTGGATAT TTTGAAGTGC ACGGTGTCCA GTTTGGAGCA TTCATACACT
    AATGCTGGCT TAGGGGGCAT GGCCAGTGTC TTCAGTCTGC TAGAGATAGC ACGCACCCAC
    TACCAAACCA AAGATCCAGA GAAGCGGAAA CGGAGCCCCA CCGAAGGGCT TAGCAGTCCA
    GGCAGTAAGG AGAGCCCATC GGGCCGAATG GAGAGTGCCA GGGCGGCAGG GGTGCTTCTG
    GTACCCCGTA TCCAATTGCC GCCACCCTCC TCTGGGAAAT CATCACGCCA GTTTGATACC
    CGGAGTCTGA ACGAGGAGAA TTTTATTGCC TCCATTGAAT TGTGGAGCAA GCACCAGGAT
    AACAGAAAGC AAAAAGCTTT GGAAAAAGAA CAGAGTGGTT TAAATAAACG GAAATCAGGA
    GCGGATGGAG TAAAACAACG TGTAGAAGGA GGAGACACTG AGGAGAAGAA ATCACAGATC
    AGTGCAGACA GTGGCCTCAG TGTGACATCT GGCTCACAGA AGAGTGATAC GGATTCCCTG
    GCGAGCTCTG AACCTCCTGC TCTGACCAGA AGCACCAGCC AGGACTCAGA GGCCAGCACA
    GTGGTAAGTA ACAGCTCAGG TGAGACGCTC GGTGCAGACA GTGACCTGAG CAGCACTGCT
    GGAGATGGCC TGACAGGAAG ACATGGTCAG CATCTCAACC TGTCCCGTGG CACACTGTCT
    GACAGTGAGA TTGAGACCAA CCCTGCAACC AGCTCAGTGT TTGGCAAGAC ACACAAGCTG
    AAGCCAGGAG TGAAGGAGCC TGTTGGTGTT AATAAAGCAG CACCTGCTCC ACCCGTTGAG
    GATGTTAGCA TGAGGATCTA TCTGTGTGAG GGGCTTCTGG GCCGGGATAA AAGCTCAGTT
    TGGGACCAGT TGGAGGATGC TGCAATGGAA ACCTTTTCTC TGAGTAAAGA GCGCTCAACT
    CTGTGGGATC AGATGCAGTT CTGGGAGGAC GCCTTCCTGG ATGCCGTAAT GCTGGAACGG
    GAAGGAATGG GAATGGATCA GGGACCACAA GAAATGATTG ACAGGTATCT TTCTCTGGGA
    GATCATGACC GCAAGAGACT GGAGGATGAT GAGGACAGAC TGCTGGCCAC GCTTCTGCAC
    AATATGATTG CTTACATGCT TATGATGAAG GTCGGTAAAA ACGACATCAG GAAGAAAGTA
    CGTCGTCTTA TGGGCAAATC CCACATTGGC CTCACTCACA GTCAGGAGAT CAATGAAGTC
    CTGGATCGCC TCGCACATTT AAGCGGTCGG GAGCTTTCTA TCAGGCCGAG TGGCAGCAGG
    CATATTAAAA AACAGACATT TGTTGTGCAT GCTGGGACAG ATACAACTGG TGACATATTC
    TTCATGGAGG TGTGTGATGA TTGCATCGTC TTGCGCAGTA ATATTGGCAC AGTCTATGAG
    CGCTGGTGGT ATGAGAAGCT CATAAATATG ACTTACTGTC CCAAAACTAA AGTGTTGTGT
    CTGTGGAGGA GAAACGGCCA GGAGACGCAG CTAAACAAGT TTTACACTAA GAAGTGTCGG
    GAATTGTACT ACTGTGTTAA AGACAGCATG GAGCGTGCTG CTGCTCGACA ACAAAGCATC
    AAACCAGGTC CAGAGCTTGG AGGCGAGTTT CCGGTTCAGG ACATGAAGAC AGGGGAGGGT
    GGTCTCCTCC AAGTCACACT GGAAGGCATC AACCTCAAAT TTATGCACAG CCAGGTTTTC
    ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTGAAAG GAGTCTTTGT CCTGGAGGAG
    TTTGTTCCTG AAACTAAAGA AGTGGTGATC CATAAGTACA AGACTCCTAT GGCACATCAG
    ATCTGTTACT CGGTGCTCTG CCTCTTCTCC TACATGGCGG CGGTCAAGGG GAAAGAGTCA
    GAGGGCAAAC CCAAGATGCT GTCCCCTCGT CCACTAGCGA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa143468
    Clone ID Related Accession (Same CDS sequence) XM_021477879.1
    Accession Version XM_021477879.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5145bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGTG TCTGGTAAGG
    TTGACTCTGA TCACCCAAAA GCAGATCTTC GCCTCCGATT CTAAGGCTCT GGCTAGCATG
    AGTCTAAACA CACAGCCTAT TCTGAACCTG GAGAAGTTTC AGGATGGACA GGAGTTGTCT
    TTATTGCCCC CCGGTCGTGA CAAAGCTTCC CCGTCCTCCA CTGAGTTCAA CCCACTCATC
    TACGGCAATG ATGTGGACTC AGTTGATGTT GCCACAAGGG TGGCAATGGT GAGGTTCTTC
    AACTCTCCAA ACGTGCTGCA AGGGTTCCAG ATGCACACAC GCACTCTCCG TCTCTTCCCT
    CGTCCTGTGG TGGCCTTTCA GTCCACATCT TTCCTGGCTT CTAGGCCACG ACGAAATGGA
    TTCACAGAAA AGCTCTCCCA CACTCAGGCA GTGGAGTACT ATGGAGAATG GGCTCTCAAC
    CCCACCAACC TGGCTTTCCA AAGGATTCAT AACAATGTGT ATGACCCCTC TCTAATAGGC
    GATAAGCCTA AGTGGTATGC CCACCAGTTA CAGCCAGTAT TCTACCGCGT GTATGATGGA
    AACTCTCATT TAGCGGAGGC CTTGAGTGGA CCCCTGCAGG ATGAGACCAA TGATTCAGAC
    CCAACAGACG ACAGTGGCAG CGACAGTGAA GCATATGATG ACTCCAGCTC CTCCTACTCA
    TCTCTTGGAG ATTTTGTGAA TGAGATGATC AAAGGGGACA TACAGGGAGA CACACCAAAT
    GTTGATCCCC TGACCCATGC TGCACTGGGA GATGCAGATG AGGTTGAGAT CCATGACTTT
    CACGAGTATA AGGGGAACAG CGGTGAACCA GAGCCAGAAG GCCCCACAGA AGCAGCGGAC
    AGTCAGCCCT TGCGGTCCAG CTCTAGCACC ACAGCCAGTT CCAGCCCCAG TACAGTCATA
    CAGGGAGTCA ACCATGAGCA GAAGGAACCT GTTGAGGTGG AGCCCATAGC AAATGTGACT
    TACCCAAGCT CTGCCCCAGT ATTGGGTCCT CCTCCTTTTA CAAGACCCAC CCCTGATCCA
    ACCCTTGTTG ACCCTGCCAA TAAGAAGCGA GAATATGACA ACCCCTATTT TGAGCCCCAG
    TACGGTTTCC CTACAGAGGA AGATGCTGAG ACTGATGAGC AGGAGGAGAG TTACACACCA
    CGATTTAACC AGAATCTCAA TGGCAATAAG CCTTCTCGTC CACTGAGACC CAGCAGCCTG
    AAGCTTCCGG GTGAATCTGA CGGAGAGGGA GACTCCAGAA ATAGCTCTCC TAACTCAACC
    ATCTCCAACA ATAGCAGTGA TGGCTTTGGG GGACTCATGT CCTTTGCCAG TAACTTGTAT
    AAAAACCATG GCACCAGTTT CAGTCTGTCC AGCCTGGCTC TGCCTAATAA AGCGAGAGAG
    AAGAACACGC CGTTTCCCAG CCTCAAAGAA TATTTTAATT TTGATTTAGC AGAGGACACA
    GATCAAGACG ATGCTGATGA CAGTCCTGAA AGTCCTGTAT TTGGGCTAAA TTCTCTAATG
    GAGATTGTAA CAGAGATCGG CCCGGGGAGC GGAGAAGGTG CCCGAGCCCC CAGAGCACTG
    GTAGATCAGA AATCCTCTGT TATTAAGCAC AGCCCTACTG TGAAGAGAGA GTCTCCGTCT
    CCTCAAGGCA GAGCCAACAA TACCAGTGAG AACCAGCAGT TTCTAAAGGA GGTGGTCCAG
    AGCGTGCTGG AGGGGCAGGG TGTGGGCTGG CTTAACATGA AGAAGGTGCG TCGCCTGCTA
    GAGAATGAAC AGCTCAGAGT CTTTGTGCTC AGCAAACTCA ACCGTGCGGT CCAGTCAGAG
    GAGGACGCCC AGCAGGAAGT TATAAGAGAT GTGGAGATAA ATAGAAAGGT CTACAAAGGC
    ATGCTGGATA TTTTGAAGTG CACGGTGTCC AGTTTGGAGC ATTCATACAC TAATGCTGGC
    TTAGGGGGCA TGGCCAGTGT CTTCAGTCTG CTAGAGATAG CACGCACCCA CTACCAAACC
    AAAGATCCAG AGAAGCGGAA ACGGAGCCCC ACCGAAGGGC TTAGCAGTCC AGGCAGTAAG
    GAGAGCCCAT CGGGCCGAAT GGAGAGTGCC AGGGCGGCAG GGGTGCTTCT GGTACCCCGT
    ATCCAATTGC CGCCACCCTC CTCTGGGAAA TCATCACGCC AGTTTGATAC CCGGAGTCTG
    AACGAGGAGA ATTTTATTGC CTCCATTGAA TTGTGGAGCA AGCACCAGGA TAACAGAAAG
    CAAAAAGCTT TGGAAAAAGA ACAGAGTGGT TTAAATAAAC GGAAATCAGG AGCGGATGGA
    GTAAAACAAC GTGTAGAAGG AGGAGACACT GAGGAGAAGA AATCACAGAT CAGTGCAGAC
    AGTGGCCTCA GTGTGACATC TGGCTCACAG AAGAGTGATA CGGATTCCCT GGCGAGCTCT
    GAACCTCCTG CTCTGACCAG AAGCACCAGC CAGGACTCAG AGGCCAGCAC AGTGGTAAGT
    AACAGCTCAG GTGAGACGCT CGGTGCAGAC AGTGACCTGA GCAGCACTGC TGGAGATGGC
    CTGACAGGAA GACATGGTCA GCATCTCAAC CTGTCCCGTG GCACACTGTC TGACAGTGAG
    ATTGAGACCA ACCCTGCAAC CAGCTCAGTG TTTGGCAAGA CACACAAGCT GAAGCCAGGA
    GTGAAGGAGC CTGTTGGTGT TAATAAAGCA GCACCTGCTC CACCCGTTGA GGATGTTAGC
    ATGAGGATCT ATCTGTGTGA GGGGCTTCTG GGCCGGGATA AAAGCTCAGT TTGGGACCAG
    TTGGAGGATG CTGCAATGGA AACCTTTTCT CTGAGTAAAG AGCGCTCAAC TCTGTGGGAT
    CAGATGCAGT TCTGGGAGGA CGCCTTCCTG GATGCCGTAA TGCTGGAACG GGAAGGAATG
    GGAATGGATC AGGGACCACA AGAAATGATT GACAGGTATC TTTCTCTGGG AGATCATGAC
    CGCAAGAGAC TGGAGGATGA TGAGGACAGA CTGCTGGCCA CGCTTCTGCA CAATATGATT
    GCTTACATGC TTATGATGAA GGTCGGTAAA AACGACATCA GGAAGAAAGT ACGTCGTCTT
    ATGGGCAAAT CCCACATTGG CCTCACTCAC AGTCAGGAGA TCAATGAAGT CCTGGATCGC
    CTCGCACATT TAAGCGGTCG GGAGCTTTCT ATCAGGCCGA GTGGCAGCAG GCATATTAAA
    AAACAGACAT TTGTTGTGCA TGCTGGGACA GATACAACTG GTGACATATT CTTCATGGAG
    GTGTGTGATG ATTGCATCGT CTTGCGCAGT AATATTGGCA CAGTCTATGA GCGCTGGTGG
    TATGAGAAGC TCATAAATAT GACTTACTGT CCCAAAACTA AAGTGTTGTG TCTGTGGAGG
    AGAAACGGCC AGGAGACGCA GCTAAACAAG TTTTACACTA AGAAGTGTCG GGAATTGTAC
    TACTGTGTTA AAGACAGCAT GGAGCGTGCT GCTGCTCGAC AACAAAGCAT CAAACCAGGT
    CCAGAGCTTG GAGGCGAGTT TCCGGTTCAG GACATGAAGA CAGGGGAGGG TGGTCTCCTC
    CAAGTCACAC TGGAAGGCAT CAACCTCAAA TTTATGCACA GCCAGGAGCG GAAGGTTTTC
    ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTGAAAG GAGTCTTTGT CCTGGAGGAG
    TTTGTTCCTG AAACTAAAGA AGTGGTGATC CATAAGTACA AGACTCCTAT GGCACATCAG
    ATCTGTTACT CGGTGCTCTG CCTCTTCTCC TACATGGCGG CGGTCAAGGG GAAAGAGTCA
    GAGGGCAAAC CCAAGATGCT GTCCCCTCGT CCACTAGCGA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333554.1
    CDS432..5576
    Translation

    Target ORF information:

    RefSeq Version XM_021477879.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021477879.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGTG TCTGGTAAGG
    TTGACTCTGA TCACCCAAAA GCAGATCTTC GCCTCCGATT CTAAGGCTCT GGCTAGCATG
    AGTCTAAACA CACAGCCTAT TCTGAACCTG GAGAAGTTTC AGGATGGACA GGAGTTGTCT
    TTATTGCCCC CCGGTCGTGA CAAAGCTTCC CCGTCCTCCA CTGAGTTCAA CCCACTCATC
    TACGGCAATG ATGTGGACTC AGTTGATGTT GCCACAAGGG TGGCAATGGT GAGGTTCTTC
    AACTCTCCAA ACGTGCTGCA AGGGTTCCAG ATGCACACAC GCACTCTCCG TCTCTTCCCT
    CGTCCTGTGG TGGCCTTTCA GTCCACATCT TTCCTGGCTT CTAGGCCACG ACGAAATGGA
    TTCACAGAAA AGCTCTCCCA CACTCAGGCA GTGGAGTACT ATGGAGAATG GGCTCTCAAC
    CCCACCAACC TGGCTTTCCA AAGGATTCAT AACAATGTGT ATGACCCCTC TCTAATAGGC
    GATAAGCCTA AGTGGTATGC CCACCAGTTA CAGCCAGTAT TCTACCGCGT GTATGATGGA
    AACTCTCATT TAGCGGAGGC CTTGAGTGGA CCCCTGCAGG ATGAGACCAA TGATTCAGAC
    CCAACAGACG ACAGTGGCAG CGACAGTGAA GCATATGATG ACTCCAGCTC CTCCTACTCA
    TCTCTTGGAG ATTTTGTGAA TGAGATGATC AAAGGGGACA TACAGGGAGA CACACCAAAT
    GTTGATCCCC TGACCCATGC TGCACTGGGA GATGCAGATG AGGTTGAGAT CCATGACTTT
    CACGAGTATA AGGGGAACAG CGGTGAACCA GAGCCAGAAG GCCCCACAGA AGCAGCGGAC
    AGTCAGCCCT TGCGGTCCAG CTCTAGCACC ACAGCCAGTT CCAGCCCCAG TACAGTCATA
    CAGGGAGTCA ACCATGAGCA GAAGGAACCT GTTGAGGTGG AGCCCATAGC AAATGTGACT
    TACCCAAGCT CTGCCCCAGT ATTGGGTCCT CCTCCTTTTA CAAGACCCAC CCCTGATCCA
    ACCCTTGTTG ACCCTGCCAA TAAGAAGCGA GAATATGACA ACCCCTATTT TGAGCCCCAG
    TACGGTTTCC CTACAGAGGA AGATGCTGAG ACTGATGAGC AGGAGGAGAG TTACACACCA
    CGATTTAACC AGAATCTCAA TGGCAATAAG CCTTCTCGTC CACTGAGACC CAGCAGCCTG
    AAGCTTCCGG GTGAATCTGA CGGAGAGGGA GACTCCAGAA ATAGCTCTCC TAACTCAACC
    ATCTCCAACA ATAGCAGTGA TGGCTTTGGG GGACTCATGT CCTTTGCCAG TAACTTGTAT
    AAAAACCATG GCACCAGTTT CAGTCTGTCC AGCCTGGCTC TGCCTAATAA AGCGAGAGAG
    AAGAACACGC CGTTTCCCAG CCTCAAAGAA TATTTTAATT TTGATTTAGC AGAGGACACA
    GATCAAGACG ATGCTGATGA CAGTCCTGAA AGTCCTGTAT TTGGGCTAAA TTCTCTAATG
    GAGATTGTAA CAGAGATCGG CCCGGGGAGC GGAGAAGGTG CCCGAGCCCC CAGAGCACTG
    GTAGATCAGA AATCCTCTGT TATTAAGCAC AGCCCTACTG TGAAGAGAGA GTCTCCGTCT
    CCTCAAGGCA GAGCCAACAA TACCAGTGAG AACCAGCAGT TTCTAAAGGA GGTGGTCCAG
    AGCGTGCTGG AGGGGCAGGG TGTGGGCTGG CTTAACATGA AGAAGGTGCG TCGCCTGCTA
    GAGAATGAAC AGCTCAGAGT CTTTGTGCTC AGCAAACTCA ACCGTGCGGT CCAGTCAGAG
    GAGGACGCCC AGCAGGAAGT TATAAGAGAT GTGGAGATAA ATAGAAAGGT CTACAAAGGC
    ATGCTGGATA TTTTGAAGTG CACGGTGTCC AGTTTGGAGC ATTCATACAC TAATGCTGGC
    TTAGGGGGCA TGGCCAGTGT CTTCAGTCTG CTAGAGATAG CACGCACCCA CTACCAAACC
    AAAGATCCAG AGAAGCGGAA ACGGAGCCCC ACCGAAGGGC TTAGCAGTCC AGGCAGTAAG
    GAGAGCCCAT CGGGCCGAAT GGAGAGTGCC AGGGCGGCAG GGGTGCTTCT GGTACCCCGT
    ATCCAATTGC CGCCACCCTC CTCTGGGAAA TCATCACGCC AGTTTGATAC CCGGAGTCTG
    AACGAGGAGA ATTTTATTGC CTCCATTGAA TTGTGGAGCA AGCACCAGGA TAACAGAAAG
    CAAAAAGCTT TGGAAAAAGA ACAGAGTGGT TTAAATAAAC GGAAATCAGG AGCGGATGGA
    GTAAAACAAC GTGTAGAAGG AGGAGACACT GAGGAGAAGA AATCACAGAT CAGTGCAGAC
    AGTGGCCTCA GTGTGACATC TGGCTCACAG AAGAGTGATA CGGATTCCCT GGCGAGCTCT
    GAACCTCCTG CTCTGACCAG AAGCACCAGC CAGGACTCAG AGGCCAGCAC AGTGGTAAGT
    AACAGCTCAG GTGAGACGCT CGGTGCAGAC AGTGACCTGA GCAGCACTGC TGGAGATGGC
    CTGACAGGAA GACATGGTCA GCATCTCAAC CTGTCCCGTG GCACACTGTC TGACAGTGAG
    ATTGAGACCA ACCCTGCAAC CAGCTCAGTG TTTGGCAAGA CACACAAGCT GAAGCCAGGA
    GTGAAGGAGC CTGTTGGTGT TAATAAAGCA GCACCTGCTC CACCCGTTGA GGATGTTAGC
    ATGAGGATCT ATCTGTGTGA GGGGCTTCTG GGCCGGGATA AAAGCTCAGT TTGGGACCAG
    TTGGAGGATG CTGCAATGGA AACCTTTTCT CTGAGTAAAG AGCGCTCAAC TCTGTGGGAT
    CAGATGCAGT TCTGGGAGGA CGCCTTCCTG GATGCCGTAA TGCTGGAACG GGAAGGAATG
    GGAATGGATC AGGGACCACA AGAAATGATT GACAGGTATC TTTCTCTGGG AGATCATGAC
    CGCAAGAGAC TGGAGGATGA TGAGGACAGA CTGCTGGCCA CGCTTCTGCA CAATATGATT
    GCTTACATGC TTATGATGAA GGTCGGTAAA AACGACATCA GGAAGAAAGT ACGTCGTCTT
    ATGGGCAAAT CCCACATTGG CCTCACTCAC AGTCAGGAGA TCAATGAAGT CCTGGATCGC
    CTCGCACATT TAAGCGGTCG GGAGCTTTCT ATCAGGCCGA GTGGCAGCAG GCATATTAAA
    AAACAGACAT TTGTTGTGCA TGCTGGGACA GATACAACTG GTGACATATT CTTCATGGAG
    GTGTGTGATG ATTGCATCGT CTTGCGCAGT AATATTGGCA CAGTCTATGA GCGCTGGTGG
    TATGAGAAGC TCATAAATAT GACTTACTGT CCCAAAACTA AAGTGTTGTG TCTGTGGAGG
    AGAAACGGCC AGGAGACGCA GCTAAACAAG TTTTACACTA AGAAGTGTCG GGAATTGTAC
    TACTGTGTTA AAGACAGCAT GGAGCGTGCT GCTGCTCGAC AACAAAGCAT CAAACCAGGT
    CCAGAGCTTG GAGGCGAGTT TCCGGTTCAG GACATGAAGA CAGGGGAGGG TGGTCTCCTC
    CAAGTCACAC TGGAAGGCAT CAACCTCAAA TTTATGCACA GCCAGGAGCG GAAGGTTTTC
    ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTGAAAG GAGTCTTTGT CCTGGAGGAG
    TTTGTTCCTG AAACTAAAGA AGTGGTGATC CATAAGTACA AGACTCCTAT GGCACATCAG
    ATCTGTTACT CGGTGCTCTG CCTCTTCTCC TACATGGCGG CGGTCAAGGG GAAAGAGTCA
    GAGGGCAAAC CCAAGATGCT GTCCCCTCGT CCACTAGCGA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa143469
    Clone ID Related Accession (Same CDS sequence) XM_021477880.1
    Accession Version XM_021477880.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5130bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA TTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGAACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCAGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT ACCCAAGCTC TGCCCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTTCCCAGC CTCAAAGAAT ATTTTAATTT TGATTTAGCA
    GAGGACACAG ATCAAGACGA TGCTGATGAC AGTCCTGAAA GTCCTGTATT TGGGCTAAAT
    TCTCTAATGG AGATTGTAAC AGAGATCGGC CCGGGGAGCG GAGAAGGTGC CCGAGCCCCC
    AGAGCACTGG TAGATCAGAA ATCCTCTGTT ATTAAGCACA GCCCTACTGT GAAGAGAGAG
    TCTCCGTCTC CTCAAGGCAG AGCCAACAAT ACCAGTGAGA ACCAGCAGTT TCTAAAGGAG
    GTGGTCCAGA GCGTGCTGGA GGGGCAGGGT GTGGGCTGGC TTAACATGAA GAAGGTGCGT
    CGCCTGCTAG AGAATGAACA GCTCAGAGTC TTTGTGCTCA GCAAACTCAA CCGTGCGGTC
    CAGTCAGAGG AGGACGCCCA GCAGGAAGTT ATAAGAGATG TGGAGATAAA TAGAAAGGTC
    TACAAAGGCA TGCTGGATAT TTTGAAGTGC ACGGTGTCCA GTTTGGAGCA TTCATACACT
    AATGCTGGCT TAGGGGGCAT GGCCAGTGTC TTCAGTCTGC TAGAGATAGC ACGCACCCAC
    TACCAAACCA AAGATCCAGA GAAGCGGAAA CGGAGCCCCA CCGAAGGGCT TAGCAGTCCA
    GGCAGTAAGG AGAGCCCATC GGGCCGAATG GAGAGTGCCA GGGCGGCAGG GGTGCTTCTG
    GTACCCCGTA TCCAATTGCC GCCACCCTCC TCTGGGAAAT CATCACGCCA GTTTGATACC
    CGGAGTCTGA ACGAGGAGAA TTTTATTGCC TCCATTGAAT TGTGGAGCAA GCACCAGGAT
    AACAGAAAGC AAAAAGCTTT GGAAAAAGAA CAGAGAGCGG ATGGAGTAAA ACAACGTGTA
    GAAGGAGGAG ACACTGAGGA GAAGAAATCA CAGATCAGTG CAGACAGTGG CCTCAGTGTG
    ACATCTGGCT CACAGAAGAG TGATACGGAT TCCCTGGCGA GCTCTGAACC TCCTGCTCTG
    ACCAGAAGCA CCAGCCAGGA CTCAGAGGCC AGCACAGTGG TAAGTAACAG CTCAGGTGAG
    ACGCTCGGTG CAGACAGTGA CCTGAGCAGC ACTGCTGGAG ATGGCCTGAC AGGAAGACAT
    GGTCAGCATC TCAACCTGTC CCGTGGCACA CTGTCTGACA GTGAGATTGA GACCAACCCT
    GCAACCAGCT CAGTGTTTGG CAAGACACAC AAGCTGAAGC CAGGAGTGAA GGAGCCTGTT
    GGTGTTAATA AAGCAGCACC TGCTCCACCC GTTGAGGATG TTAGCATGAG GATCTATCTG
    TGTGAGGGGC TTCTGGGCCG GGATAAAAGC TCAGTTTGGG ACCAGTTGGA GGATGCTGCA
    ATGGAAACCT TTTCTCTGAG TAAAGAGCGC TCAACTCTGT GGGATCAGAT GCAGTTCTGG
    GAGGACGCCT TCCTGGATGC CGTAATGCTG GAACGGGAAG GAATGGGAAT GGATCAGGGA
    CCACAAGAAA TGATTGACAG GTATCTTTCT CTGGGAGATC ATGACCGCAA GAGACTGGAG
    GATGATGAGG ACAGACTGCT GGCCACGCTT CTGCACAATA TGATTGCTTA CATGCTTATG
    ATGAAGGTCG GTAAAAACGA CATCAGGAAG AAAGTACGTC GTCTTATGGG CAAATCCCAC
    ATTGGCCTCA CTCACAGTCA GGAGATCAAT GAAGTCCTGG ATCGCCTCGC ACATTTAAGC
    GGTCGGGAGC TTTCTATCAG GCCGAGTGGC AGCAGGCATA TTAAAAAACA GACATTTGTT
    GTGCATGCTG GGACAGATAC AACTGGTGAC ATATTCTTCA TGGAGGTGTG TGATGATTGC
    ATCGTCTTGC GCAGTAATAT TGGCACAGTC TATGAGCGCT GGTGGTATGA GAAGCTCATA
    AATATGACTT ACTGTCCCAA AACTAAAGTG TTGTGTCTGT GGAGGAGAAA CGGCCAGGAG
    ACGCAGCTAA ACAAGTTTTA CACTAAGAAG TGTCGGGAAT TGTACTACTG TGTTAAAGAC
    AGCATGGAGC GTGCTGCTGC TCGACAACAA AGCATCAAAC CAGGTCCAGA GCTTGGAGGC
    GAGTTTCCGG TTCAGGACAT GAAGACAGGG GAGGGTGGTC TCCTCCAAGT CACACTGGAA
    GGCATCAACC TCAAATTTAT GCACAGCCAG GAGCGGAAGG TTTTCATAGA GCTGAATCAC
    ATTAAAAAGT GCAATACAGT GAAAGGAGTC TTTGTCCTGG AGGAGTTTGT TCCTGAAACT
    AAAGAAGTGG TGATCCATAA GTACAAGACT CCTATGGCAC ATCAGATCTG TTACTCGGTG
    CTCTGCCTCT TCTCCTACAT GGCGGCGGTC AAGGGGAAAG AGTCAGAGGG CAAACCCAAG
    ATGCTGTCCC CTCGTCCACT AGCGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333555.1
    CDS432..5561
    Translation

    Target ORF information:

    RefSeq Version XM_021477880.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021477880.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA TTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGAACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCAGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT ACCCAAGCTC TGCCCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTTCCCAGC CTCAAAGAAT ATTTTAATTT TGATTTAGCA
    GAGGACACAG ATCAAGACGA TGCTGATGAC AGTCCTGAAA GTCCTGTATT TGGGCTAAAT
    TCTCTAATGG AGATTGTAAC AGAGATCGGC CCGGGGAGCG GAGAAGGTGC CCGAGCCCCC
    AGAGCACTGG TAGATCAGAA ATCCTCTGTT ATTAAGCACA GCCCTACTGT GAAGAGAGAG
    TCTCCGTCTC CTCAAGGCAG AGCCAACAAT ACCAGTGAGA ACCAGCAGTT TCTAAAGGAG
    GTGGTCCAGA GCGTGCTGGA GGGGCAGGGT GTGGGCTGGC TTAACATGAA GAAGGTGCGT
    CGCCTGCTAG AGAATGAACA GCTCAGAGTC TTTGTGCTCA GCAAACTCAA CCGTGCGGTC
    CAGTCAGAGG AGGACGCCCA GCAGGAAGTT ATAAGAGATG TGGAGATAAA TAGAAAGGTC
    TACAAAGGCA TGCTGGATAT TTTGAAGTGC ACGGTGTCCA GTTTGGAGCA TTCATACACT
    AATGCTGGCT TAGGGGGCAT GGCCAGTGTC TTCAGTCTGC TAGAGATAGC ACGCACCCAC
    TACCAAACCA AAGATCCAGA GAAGCGGAAA CGGAGCCCCA CCGAAGGGCT TAGCAGTCCA
    GGCAGTAAGG AGAGCCCATC GGGCCGAATG GAGAGTGCCA GGGCGGCAGG GGTGCTTCTG
    GTACCCCGTA TCCAATTGCC GCCACCCTCC TCTGGGAAAT CATCACGCCA GTTTGATACC
    CGGAGTCTGA ACGAGGAGAA TTTTATTGCC TCCATTGAAT TGTGGAGCAA GCACCAGGAT
    AACAGAAAGC AAAAAGCTTT GGAAAAAGAA CAGAGAGCGG ATGGAGTAAA ACAACGTGTA
    GAAGGAGGAG ACACTGAGGA GAAGAAATCA CAGATCAGTG CAGACAGTGG CCTCAGTGTG
    ACATCTGGCT CACAGAAGAG TGATACGGAT TCCCTGGCGA GCTCTGAACC TCCTGCTCTG
    ACCAGAAGCA CCAGCCAGGA CTCAGAGGCC AGCACAGTGG TAAGTAACAG CTCAGGTGAG
    ACGCTCGGTG CAGACAGTGA CCTGAGCAGC ACTGCTGGAG ATGGCCTGAC AGGAAGACAT
    GGTCAGCATC TCAACCTGTC CCGTGGCACA CTGTCTGACA GTGAGATTGA GACCAACCCT
    GCAACCAGCT CAGTGTTTGG CAAGACACAC AAGCTGAAGC CAGGAGTGAA GGAGCCTGTT
    GGTGTTAATA AAGCAGCACC TGCTCCACCC GTTGAGGATG TTAGCATGAG GATCTATCTG
    TGTGAGGGGC TTCTGGGCCG GGATAAAAGC TCAGTTTGGG ACCAGTTGGA GGATGCTGCA
    ATGGAAACCT TTTCTCTGAG TAAAGAGCGC TCAACTCTGT GGGATCAGAT GCAGTTCTGG
    GAGGACGCCT TCCTGGATGC CGTAATGCTG GAACGGGAAG GAATGGGAAT GGATCAGGGA
    CCACAAGAAA TGATTGACAG GTATCTTTCT CTGGGAGATC ATGACCGCAA GAGACTGGAG
    GATGATGAGG ACAGACTGCT GGCCACGCTT CTGCACAATA TGATTGCTTA CATGCTTATG
    ATGAAGGTCG GTAAAAACGA CATCAGGAAG AAAGTACGTC GTCTTATGGG CAAATCCCAC
    ATTGGCCTCA CTCACAGTCA GGAGATCAAT GAAGTCCTGG ATCGCCTCGC ACATTTAAGC
    GGTCGGGAGC TTTCTATCAG GCCGAGTGGC AGCAGGCATA TTAAAAAACA GACATTTGTT
    GTGCATGCTG GGACAGATAC AACTGGTGAC ATATTCTTCA TGGAGGTGTG TGATGATTGC
    ATCGTCTTGC GCAGTAATAT TGGCACAGTC TATGAGCGCT GGTGGTATGA GAAGCTCATA
    AATATGACTT ACTGTCCCAA AACTAAAGTG TTGTGTCTGT GGAGGAGAAA CGGCCAGGAG
    ACGCAGCTAA ACAAGTTTTA CACTAAGAAG TGTCGGGAAT TGTACTACTG TGTTAAAGAC
    AGCATGGAGC GTGCTGCTGC TCGACAACAA AGCATCAAAC CAGGTCCAGA GCTTGGAGGC
    GAGTTTCCGG TTCAGGACAT GAAGACAGGG GAGGGTGGTC TCCTCCAAGT CACACTGGAA
    GGCATCAACC TCAAATTTAT GCACAGCCAG GAGCGGAAGG TTTTCATAGA GCTGAATCAC
    ATTAAAAAGT GCAATACAGT GAAAGGAGTC TTTGTCCTGG AGGAGTTTGT TCCTGAAACT
    AAAGAAGTGG TGATCCATAA GTACAAGACT CCTATGGCAC ATCAGATCTG TTACTCGGTG
    CTCTGCCTCT TCTCCTACAT GGCGGCGGTC AAGGGGAAAG AGTCAGAGGG CAAACCCAAG
    ATGCTGTCCC CTCGTCCACT AGCGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa143470
    Clone ID Related Accession (Same CDS sequence) XM_021477881.1
    Accession Version XM_021477881.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5124bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA TTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGAACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCAGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT ACCCAAGCTC TGCCCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTTCCCAGC CTCAAAGAAT ATTTTAATTT TGATTTAGCA
    GAGGACACAG ATCAAGTATT TGGGCTAAAT TCTCTAATGG AGATTGTAAC AGAGATCGGC
    CCGGGGAGCG GAGAAGGTGC CCGAGCCCCC AGAGCACTGG TAGATCAGAA ATCCTCTGTT
    ATTAAGCACA GCCCTACTGT GAAGAGAGAG TCTCCGTCTC CTCAAGGCAG AGCCAACAAT
    ACCAGTGAGA ACCAGCAGTT TCTAAAGGAG GTGGTCCAGA GCGTGCTGGA GGGGCAGGGT
    GTGGGCTGGC TTAACATGAA GAAGGTGCGT CGCCTGCTAG AGAATGAACA GCTCAGAGTC
    TTTGTGCTCA GCAAACTCAA CCGTGCGGTC CAGTCAGAGG AGGACGCCCA GCAGGAAGTT
    ATAAGAGATG TGGAGATAAA TAGAAAGGTC TACAAAGGCA TGCTGGATAT TTTGAAGTGC
    ACGGTGTCCA GTTTGGAGCA TTCATACACT AATGCTGGCT TAGGGGGCAT GGCCAGTGTC
    TTCAGTCTGC TAGAGATAGC ACGCACCCAC TACCAAACCA AAGATCCAGA GAAGCGGAAA
    CGGAGCCCCA CCGAAGGGCT TAGCAGTCCA GGCAGTAAGG AGAGCCCATC GGGCCGAATG
    GAGAGTGCCA GGGCGGCAGG GGTGCTTCTG GTACCCCGTA TCCAATTGCC GCCACCCTCC
    TCTGGGAAAT CATCACGCCA GTTTGATACC CGGAGTCTGA ACGAGGAGAA TTTTATTGCC
    TCCATTGAAT TGTGGAGCAA GCACCAGGAT AACAGAAAGC AAAAAGCTTT GGAAAAAGAA
    CAGAGTGGTT TAAATAAACG GAAATCAGGA GCGGATGGAG TAAAACAACG TGTAGAAGGA
    GGAGACACTG AGGAGAAGAA ATCACAGATC AGTGCAGACA GTGGCCTCAG TGTGACATCT
    GGCTCACAGA AGAGTGATAC GGATTCCCTG GCGAGCTCTG AACCTCCTGC TCTGACCAGA
    AGCACCAGCC AGGACTCAGA GGCCAGCACA GTGGTAAGTA ACAGCTCAGG TGAGACGCTC
    GGTGCAGACA GTGACCTGAG CAGCACTGCT GGAGATGGCC TGACAGGAAG ACATGGTCAG
    CATCTCAACC TGTCCCGTGG CACACTGTCT GACAGTGAGA TTGAGACCAA CCCTGCAACC
    AGCTCAGTGT TTGGCAAGAC ACACAAGCTG AAGCCAGGAG TGAAGGAGCC TGTTGGTGTT
    AATAAAGCAG CACCTGCTCC ACCCGTTGAG GATGTTAGCA TGAGGATCTA TCTGTGTGAG
    GGGCTTCTGG GCCGGGATAA AAGCTCAGTT TGGGACCAGT TGGAGGATGC TGCAATGGAA
    ACCTTTTCTC TGAGTAAAGA GCGCTCAACT CTGTGGGATC AGATGCAGTT CTGGGAGGAC
    GCCTTCCTGG ATGCCGTAAT GCTGGAACGG GAAGGAATGG GAATGGATCA GGGACCACAA
    GAAATGATTG ACAGGTATCT TTCTCTGGGA GATCATGACC GCAAGAGACT GGAGGATGAT
    GAGGACAGAC TGCTGGCCAC GCTTCTGCAC AATATGATTG CTTACATGCT TATGATGAAG
    GTCGGTAAAA ACGACATCAG GAAGAAAGTA CGTCGTCTTA TGGGCAAATC CCACATTGGC
    CTCACTCACA GTCAGGAGAT CAATGAAGTC CTGGATCGCC TCGCACATTT AAGCGGTCGG
    GAGCTTTCTA TCAGGCCGAG TGGCAGCAGG CATATTAAAA AACAGACATT TGTTGTGCAT
    GCTGGGACAG ATACAACTGG TGACATATTC TTCATGGAGG TGTGTGATGA TTGCATCGTC
    TTGCGCAGTA ATATTGGCAC AGTCTATGAG CGCTGGTGGT ATGAGAAGCT CATAAATATG
    ACTTACTGTC CCAAAACTAA AGTGTTGTGT CTGTGGAGGA GAAACGGCCA GGAGACGCAG
    CTAAACAAGT TTTACACTAA GAAGTGTCGG GAATTGTACT ACTGTGTTAA AGACAGCATG
    GAGCGTGCTG CTGCTCGACA ACAAAGCATC AAACCAGGTC CAGAGCTTGG AGGCGAGTTT
    CCGGTTCAGG ACATGAAGAC AGGGGAGGGT GGTCTCCTCC AAGTCACACT GGAAGGCATC
    AACCTCAAAT TTATGCACAG CCAGGAGCGG AAGGTTTTCA TAGAGCTGAA TCACATTAAA
    AAGTGCAATA CAGTGAAAGG AGTCTTTGTC CTGGAGGAGT TTGTTCCTGA AACTAAAGAA
    GTGGTGATCC ATAAGTACAA GACTCCTATG GCACATCAGA TCTGTTACTC GGTGCTCTGC
    CTCTTCTCCT ACATGGCGGC GGTCAAGGGG AAAGAGTCAG AGGGCAAACC CAAGATGCTG
    TCCCCTCGTC CACTAGCGAG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333556.1
    CDS432..5555
    Translation

    Target ORF information:

    RefSeq Version XM_021477881.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021477881.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA TTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGAACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCAGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT ACCCAAGCTC TGCCCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTTCCCAGC CTCAAAGAAT ATTTTAATTT TGATTTAGCA
    GAGGACACAG ATCAAGTATT TGGGCTAAAT TCTCTAATGG AGATTGTAAC AGAGATCGGC
    CCGGGGAGCG GAGAAGGTGC CCGAGCCCCC AGAGCACTGG TAGATCAGAA ATCCTCTGTT
    ATTAAGCACA GCCCTACTGT GAAGAGAGAG TCTCCGTCTC CTCAAGGCAG AGCCAACAAT
    ACCAGTGAGA ACCAGCAGTT TCTAAAGGAG GTGGTCCAGA GCGTGCTGGA GGGGCAGGGT
    GTGGGCTGGC TTAACATGAA GAAGGTGCGT CGCCTGCTAG AGAATGAACA GCTCAGAGTC
    TTTGTGCTCA GCAAACTCAA CCGTGCGGTC CAGTCAGAGG AGGACGCCCA GCAGGAAGTT
    ATAAGAGATG TGGAGATAAA TAGAAAGGTC TACAAAGGCA TGCTGGATAT TTTGAAGTGC
    ACGGTGTCCA GTTTGGAGCA TTCATACACT AATGCTGGCT TAGGGGGCAT GGCCAGTGTC
    TTCAGTCTGC TAGAGATAGC ACGCACCCAC TACCAAACCA AAGATCCAGA GAAGCGGAAA
    CGGAGCCCCA CCGAAGGGCT TAGCAGTCCA GGCAGTAAGG AGAGCCCATC GGGCCGAATG
    GAGAGTGCCA GGGCGGCAGG GGTGCTTCTG GTACCCCGTA TCCAATTGCC GCCACCCTCC
    TCTGGGAAAT CATCACGCCA GTTTGATACC CGGAGTCTGA ACGAGGAGAA TTTTATTGCC
    TCCATTGAAT TGTGGAGCAA GCACCAGGAT AACAGAAAGC AAAAAGCTTT GGAAAAAGAA
    CAGAGTGGTT TAAATAAACG GAAATCAGGA GCGGATGGAG TAAAACAACG TGTAGAAGGA
    GGAGACACTG AGGAGAAGAA ATCACAGATC AGTGCAGACA GTGGCCTCAG TGTGACATCT
    GGCTCACAGA AGAGTGATAC GGATTCCCTG GCGAGCTCTG AACCTCCTGC TCTGACCAGA
    AGCACCAGCC AGGACTCAGA GGCCAGCACA GTGGTAAGTA ACAGCTCAGG TGAGACGCTC
    GGTGCAGACA GTGACCTGAG CAGCACTGCT GGAGATGGCC TGACAGGAAG ACATGGTCAG
    CATCTCAACC TGTCCCGTGG CACACTGTCT GACAGTGAGA TTGAGACCAA CCCTGCAACC
    AGCTCAGTGT TTGGCAAGAC ACACAAGCTG AAGCCAGGAG TGAAGGAGCC TGTTGGTGTT
    AATAAAGCAG CACCTGCTCC ACCCGTTGAG GATGTTAGCA TGAGGATCTA TCTGTGTGAG
    GGGCTTCTGG GCCGGGATAA AAGCTCAGTT TGGGACCAGT TGGAGGATGC TGCAATGGAA
    ACCTTTTCTC TGAGTAAAGA GCGCTCAACT CTGTGGGATC AGATGCAGTT CTGGGAGGAC
    GCCTTCCTGG ATGCCGTAAT GCTGGAACGG GAAGGAATGG GAATGGATCA GGGACCACAA
    GAAATGATTG ACAGGTATCT TTCTCTGGGA GATCATGACC GCAAGAGACT GGAGGATGAT
    GAGGACAGAC TGCTGGCCAC GCTTCTGCAC AATATGATTG CTTACATGCT TATGATGAAG
    GTCGGTAAAA ACGACATCAG GAAGAAAGTA CGTCGTCTTA TGGGCAAATC CCACATTGGC
    CTCACTCACA GTCAGGAGAT CAATGAAGTC CTGGATCGCC TCGCACATTT AAGCGGTCGG
    GAGCTTTCTA TCAGGCCGAG TGGCAGCAGG CATATTAAAA AACAGACATT TGTTGTGCAT
    GCTGGGACAG ATACAACTGG TGACATATTC TTCATGGAGG TGTGTGATGA TTGCATCGTC
    TTGCGCAGTA ATATTGGCAC AGTCTATGAG CGCTGGTGGT ATGAGAAGCT CATAAATATG
    ACTTACTGTC CCAAAACTAA AGTGTTGTGT CTGTGGAGGA GAAACGGCCA GGAGACGCAG
    CTAAACAAGT TTTACACTAA GAAGTGTCGG GAATTGTACT ACTGTGTTAA AGACAGCATG
    GAGCGTGCTG CTGCTCGACA ACAAAGCATC AAACCAGGTC CAGAGCTTGG AGGCGAGTTT
    CCGGTTCAGG ACATGAAGAC AGGGGAGGGT GGTCTCCTCC AAGTCACACT GGAAGGCATC
    AACCTCAAAT TTATGCACAG CCAGGAGCGG AAGGTTTTCA TAGAGCTGAA TCACATTAAA
    AAGTGCAATA CAGTGAAAGG AGTCTTTGTC CTGGAGGAGT TTGTTCCTGA AACTAAAGAA
    GTGGTGATCC ATAAGTACAA GACTCCTATG GCACATCAGA TCTGTTACTC GGTGCTCTGC
    CTCTTCTCCT ACATGGCGGC GGTCAAGGGG AAAGAGTCAG AGGGCAAACC CAAGATGCTG
    TCCCCTCGTC CACTAGCGAG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa143471
    Clone ID Related Accession (Same CDS sequence) XM_021477882.1
    Accession Version XM_021477882.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5115bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA TTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGAACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCAGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT ACCCAAGCTC TGCCCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTTCCCAGC CTCAAAGACG ATGCTGATGA CAGTCCTGAA
    AGTCCTGTAT TTGGGCTAAA TTCTCTAATG GAGATTGTAA CAGAGATCGG CCCGGGGAGC
    GGAGAAGGTG CCCGAGCCCC CAGAGCACTG GTAGATCAGA AATCCTCTGT TATTAAGCAC
    AGCCCTACTG TGAAGAGAGA GTCTCCGTCT CCTCAAGGCA GAGCCAACAA TACCAGTGAG
    AACCAGCAGT TTCTAAAGGA GGTGGTCCAG AGCGTGCTGG AGGGGCAGGG TGTGGGCTGG
    CTTAACATGA AGAAGGTGCG TCGCCTGCTA GAGAATGAAC AGCTCAGAGT CTTTGTGCTC
    AGCAAACTCA ACCGTGCGGT CCAGTCAGAG GAGGACGCCC AGCAGGAAGT TATAAGAGAT
    GTGGAGATAA ATAGAAAGGT CTACAAAGGC ATGCTGGATA TTTTGAAGTG CACGGTGTCC
    AGTTTGGAGC ATTCATACAC TAATGCTGGC TTAGGGGGCA TGGCCAGTGT CTTCAGTCTG
    CTAGAGATAG CACGCACCCA CTACCAAACC AAAGATCCAG AGAAGCGGAA ACGGAGCCCC
    ACCGAAGGGC TTAGCAGTCC AGGCAGTAAG GAGAGCCCAT CGGGCCGAAT GGAGAGTGCC
    AGGGCGGCAG GGGTGCTTCT GGTACCCCGT ATCCAATTGC CGCCACCCTC CTCTGGGAAA
    TCATCACGCC AGTTTGATAC CCGGAGTCTG AACGAGGAGA ATTTTATTGC CTCCATTGAA
    TTGTGGAGCA AGCACCAGGA TAACAGAAAG CAAAAAGCTT TGGAAAAAGA ACAGAGTGGT
    TTAAATAAAC GGAAATCAGG AGCGGATGGA GTAAAACAAC GTGTAGAAGG AGGAGACACT
    GAGGAGAAGA AATCACAGAT CAGTGCAGAC AGTGGCCTCA GTGTGACATC TGGCTCACAG
    AAGAGTGATA CGGATTCCCT GGCGAGCTCT GAACCTCCTG CTCTGACCAG AAGCACCAGC
    CAGGACTCAG AGGCCAGCAC AGTGGTAAGT AACAGCTCAG GTGAGACGCT CGGTGCAGAC
    AGTGACCTGA GCAGCACTGC TGGAGATGGC CTGACAGGAA GACATGGTCA GCATCTCAAC
    CTGTCCCGTG GCACACTGTC TGACAGTGAG ATTGAGACCA ACCCTGCAAC CAGCTCAGTG
    TTTGGCAAGA CACACAAGCT GAAGCCAGGA GTGAAGGAGC CTGTTGGTGT TAATAAAGCA
    GCACCTGCTC CACCCGTTGA GGATGTTAGC ATGAGGATCT ATCTGTGTGA GGGGCTTCTG
    GGCCGGGATA AAAGCTCAGT TTGGGACCAG TTGGAGGATG CTGCAATGGA AACCTTTTCT
    CTGAGTAAAG AGCGCTCAAC TCTGTGGGAT CAGATGCAGT TCTGGGAGGA CGCCTTCCTG
    GATGCCGTAA TGCTGGAACG GGAAGGAATG GGAATGGATC AGGGACCACA AGAAATGATT
    GACAGGTATC TTTCTCTGGG AGATCATGAC CGCAAGAGAC TGGAGGATGA TGAGGACAGA
    CTGCTGGCCA CGCTTCTGCA CAATATGATT GCTTACATGC TTATGATGAA GGTCGGTAAA
    AACGACATCA GGAAGAAAGT ACGTCGTCTT ATGGGCAAAT CCCACATTGG CCTCACTCAC
    AGTCAGGAGA TCAATGAAGT CCTGGATCGC CTCGCACATT TAAGCGGTCG GGAGCTTTCT
    ATCAGGCCGA GTGGCAGCAG GCATATTAAA AAACAGACAT TTGTTGTGCA TGCTGGGACA
    GATACAACTG GTGACATATT CTTCATGGAG GTGTGTGATG ATTGCATCGT CTTGCGCAGT
    AATATTGGCA CAGTCTATGA GCGCTGGTGG TATGAGAAGC TCATAAATAT GACTTACTGT
    CCCAAAACTA AAGTGTTGTG TCTGTGGAGG AGAAACGGCC AGGAGACGCA GCTAAACAAG
    TTTTACACTA AGAAGTGTCG GGAATTGTAC TACTGTGTTA AAGACAGCAT GGAGCGTGCT
    GCTGCTCGAC AACAAAGCAT CAAACCAGGT CCAGAGCTTG GAGGCGAGTT TCCGGTTCAG
    GACATGAAGA CAGGGGAGGG TGGTCTCCTC CAAGTCACAC TGGAAGGCAT CAACCTCAAA
    TTTATGCACA GCCAGGAGCG GAAGGTTTTC ATAGAGCTGA ATCACATTAA AAAGTGCAAT
    ACAGTGAAAG GAGTCTTTGT CCTGGAGGAG TTTGTTCCTG AAACTAAAGA AGTGGTGATC
    CATAAGTACA AGACTCCTAT GGCACATCAG ATCTGTTACT CGGTGCTCTG CCTCTTCTCC
    TACATGGCGG CGGTCAAGGG GAAAGAGTCA GAGGGCAAAC CCAAGATGCT GTCCCCTCGT
    CCACTAGCGA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333557.1
    CDS432..5546
    Translation

    Target ORF information:

    RefSeq Version XM_021477882.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021477882.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA TTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGAACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCAGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT ACCCAAGCTC TGCCCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTTCCCAGC CTCAAAGACG ATGCTGATGA CAGTCCTGAA
    AGTCCTGTAT TTGGGCTAAA TTCTCTAATG GAGATTGTAA CAGAGATCGG CCCGGGGAGC
    GGAGAAGGTG CCCGAGCCCC CAGAGCACTG GTAGATCAGA AATCCTCTGT TATTAAGCAC
    AGCCCTACTG TGAAGAGAGA GTCTCCGTCT CCTCAAGGCA GAGCCAACAA TACCAGTGAG
    AACCAGCAGT TTCTAAAGGA GGTGGTCCAG AGCGTGCTGG AGGGGCAGGG TGTGGGCTGG
    CTTAACATGA AGAAGGTGCG TCGCCTGCTA GAGAATGAAC AGCTCAGAGT CTTTGTGCTC
    AGCAAACTCA ACCGTGCGGT CCAGTCAGAG GAGGACGCCC AGCAGGAAGT TATAAGAGAT
    GTGGAGATAA ATAGAAAGGT CTACAAAGGC ATGCTGGATA TTTTGAAGTG CACGGTGTCC
    AGTTTGGAGC ATTCATACAC TAATGCTGGC TTAGGGGGCA TGGCCAGTGT CTTCAGTCTG
    CTAGAGATAG CACGCACCCA CTACCAAACC AAAGATCCAG AGAAGCGGAA ACGGAGCCCC
    ACCGAAGGGC TTAGCAGTCC AGGCAGTAAG GAGAGCCCAT CGGGCCGAAT GGAGAGTGCC
    AGGGCGGCAG GGGTGCTTCT GGTACCCCGT ATCCAATTGC CGCCACCCTC CTCTGGGAAA
    TCATCACGCC AGTTTGATAC CCGGAGTCTG AACGAGGAGA ATTTTATTGC CTCCATTGAA
    TTGTGGAGCA AGCACCAGGA TAACAGAAAG CAAAAAGCTT TGGAAAAAGA ACAGAGTGGT
    TTAAATAAAC GGAAATCAGG AGCGGATGGA GTAAAACAAC GTGTAGAAGG AGGAGACACT
    GAGGAGAAGA AATCACAGAT CAGTGCAGAC AGTGGCCTCA GTGTGACATC TGGCTCACAG
    AAGAGTGATA CGGATTCCCT GGCGAGCTCT GAACCTCCTG CTCTGACCAG AAGCACCAGC
    CAGGACTCAG AGGCCAGCAC AGTGGTAAGT AACAGCTCAG GTGAGACGCT CGGTGCAGAC
    AGTGACCTGA GCAGCACTGC TGGAGATGGC CTGACAGGAA GACATGGTCA GCATCTCAAC
    CTGTCCCGTG GCACACTGTC TGACAGTGAG ATTGAGACCA ACCCTGCAAC CAGCTCAGTG
    TTTGGCAAGA CACACAAGCT GAAGCCAGGA GTGAAGGAGC CTGTTGGTGT TAATAAAGCA
    GCACCTGCTC CACCCGTTGA GGATGTTAGC ATGAGGATCT ATCTGTGTGA GGGGCTTCTG
    GGCCGGGATA AAAGCTCAGT TTGGGACCAG TTGGAGGATG CTGCAATGGA AACCTTTTCT
    CTGAGTAAAG AGCGCTCAAC TCTGTGGGAT CAGATGCAGT TCTGGGAGGA CGCCTTCCTG
    GATGCCGTAA TGCTGGAACG GGAAGGAATG GGAATGGATC AGGGACCACA AGAAATGATT
    GACAGGTATC TTTCTCTGGG AGATCATGAC CGCAAGAGAC TGGAGGATGA TGAGGACAGA
    CTGCTGGCCA CGCTTCTGCA CAATATGATT GCTTACATGC TTATGATGAA GGTCGGTAAA
    AACGACATCA GGAAGAAAGT ACGTCGTCTT ATGGGCAAAT CCCACATTGG CCTCACTCAC
    AGTCAGGAGA TCAATGAAGT CCTGGATCGC CTCGCACATT TAAGCGGTCG GGAGCTTTCT
    ATCAGGCCGA GTGGCAGCAG GCATATTAAA AAACAGACAT TTGTTGTGCA TGCTGGGACA
    GATACAACTG GTGACATATT CTTCATGGAG GTGTGTGATG ATTGCATCGT CTTGCGCAGT
    AATATTGGCA CAGTCTATGA GCGCTGGTGG TATGAGAAGC TCATAAATAT GACTTACTGT
    CCCAAAACTA AAGTGTTGTG TCTGTGGAGG AGAAACGGCC AGGAGACGCA GCTAAACAAG
    TTTTACACTA AGAAGTGTCG GGAATTGTAC TACTGTGTTA AAGACAGCAT GGAGCGTGCT
    GCTGCTCGAC AACAAAGCAT CAAACCAGGT CCAGAGCTTG GAGGCGAGTT TCCGGTTCAG
    GACATGAAGA CAGGGGAGGG TGGTCTCCTC CAAGTCACAC TGGAAGGCAT CAACCTCAAA
    TTTATGCACA GCCAGGAGCG GAAGGTTTTC ATAGAGCTGA ATCACATTAA AAAGTGCAAT
    ACAGTGAAAG GAGTCTTTGT CCTGGAGGAG TTTGTTCCTG AAACTAAAGA AGTGGTGATC
    CATAAGTACA AGACTCCTAT GGCACATCAG ATCTGTTACT CGGTGCTCTG CCTCTTCTCC
    TACATGGCGG CGGTCAAGGG GAAAGAGTCA GAGGGCAAAC CCAAGATGCT GTCCCCTCGT
    CCACTAGCGA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa143472
    Clone ID Related Accession (Same CDS sequence) XM_021477883.1
    Accession Version XM_021477883.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5097bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGGCT
    CTGGCTAGCA TGAGTCTAAA CACACAGCCT ATTCTGAACC TGGAGAAGTT TCAGGATGGA
    CAGGAGTTGT CTTTATTGCC CCCCGGTCGT GACAAAGCTT CCCCGTCCTC CACTGAGTTC
    AACCCACTCA TCTACGGCAA TGATGTGGAC TCAGTTGATG TTGCCACAAG GGTGGCAATG
    GTGAGGTTCT TCAACTCTCC AAACGTGCTG CAAGGGTTCC AGATGCACAC ACGCACTCTC
    CGTCTCTTCC CTCGTCCTGT GGTGGCCTTT CAGTCCACAT CTTTCCTGGC TTCTAGGCCA
    CGACGAAATG GATTCACAGA AAAGCTCTCC CACACTCAGG CAGTGGAGTA CTATGGAGAA
    TGGGCTCTCA ACCCCACCAA CCTGGCTTTC CAAAGGATTC ATAACAATGT GTATGACCCC
    TCTCTAATAG GCGATAAGCC TAAGTGGTAT GCCCACCAGT TACAGCCAGT ATTCTACCGC
    GTGTATGATG GAAACTCTCA TTTAGCGGAG GCCTTGAGTG GACCCCTGCA GGATGAGACC
    AATGATTCAG ACCCAACAGA CGACAGTGGC AGCGACAGTG AAGCATATGA TGACTCCAGC
    TCCTCCTACT CATCTCTTGG AGATTTTGTG AATGAGATGA TCAAAGGGGA CATACAGGGA
    GACACACCAA ATGTTGATCC CCTGACCCAT GCTGCACTGG GAGATGCAGA TGAGGTTGAG
    ATCCATGACT TTCACGAGTA TAAGGGGAAC AGCGGTGAAC CAGAGCCAGA AGGCCCCACA
    GAAGCAGCGG ACAGTCAGCC CTTGCGGTCC AGCTCTAGCA CCACAGCCAG TTCCAGCCCC
    AGTACAGTCA TACAGGGAGT CAACCATGAG CAGAAGGAAC CTGTTGAGGT GGAGCCCATA
    GCAAATGTGA CTTACCCAAG CTCTGCCCCA GTATTGGGTC CTCCTCCTTT TACAAGACCC
    ACCCCTGATC CAACCCTTGT TGACCCTGCC AATAAGAAGC GAGAATATGA CAACCCCTAT
    TTTGAGCCCC AGTACGGTTT CCCTACAGAG GAAGATGCTG AGACTGATGA GCAGGAGGAG
    AGTTACACAC CACGATTTAA CCAGAATCTC AATGGCAATA AGCCTTCTCG TCCACTGAGA
    CCCAGCAGCC TGAAGCTTCC GGGTGAATCT GACGGAGAGG GAGACTCCAG AAATAGCTCT
    CCTAACTCAA CCATCTCCAA CAATAGCAGT GATGGCTTTG GGGGACTCAT GTCCTTTGCC
    AGTAACTTGT ATAAAAACCA TGGCACCAGT TTCAGTCTGT CCAGCCTGGC TCTGCCTAAT
    AAAGCGAGAG AGAAGAACAC GCCGTTTCCC AGCCTCAAAG AATATTTTAA TTTTGATTTA
    GCAGAGGACA CAGATCAAGA CGATGCTGAT GACAGTCCTG AAAGTCCTGT ATTTGGGCTA
    AATTCTCTAA TGGAGATTGT AACAGAGATC GGCCCGGGGA GCGGAGAAGG TGCCCGAGCC
    CCCAGAGCAC TGGTAGATCA GAAATCCTCT GTTATTAAGC ACAGCCCTAC TGTGAAGAGA
    GAGTCTCCGT CTCCTCAAGG CAGAGCCAAC AATACCAGTG AGAACCAGCA GTTTCTAAAG
    GAGGTGGTCC AGAGCGTGCT GGAGGGGCAG GGTGTGGGCT GGCTTAACAT GAAGAAGGTG
    CGTCGCCTGC TAGAGAATGA ACAGCTCAGA GTCTTTGTGC TCAGCAAACT CAACCGTGCG
    GTCCAGTCAG AGGAGGACGC CCAGCAGGAA GTTATAAGAG ATGTGGAGAT AAATAGAAAG
    GTCTACAAAG GCATGCTGGA TATTTTGAAG TGCACGGTGT CCAGTTTGGA GCATTCATAC
    ACTAATGCTG GCTTAGGGGG CATGGCCAGT GTCTTCAGTC TGCTAGAGAT AGCACGCACC
    CACTACCAAA CCAAAGATCC AGAGAAGCGG AAACGGAGCC CCACCGAAGG GCTTAGCAGT
    CCAGGCAGTA AGGAGAGCCC ATCGGGCCGA ATGGAGAGTG CCAGGGCGGC AGGGGTGCTT
    CTGGTACCCC GTATCCAATT GCCGCCACCC TCCTCTGGGA AATCATCACG CCAGTTTGAT
    ACCCGGAGTC TGAACGAGGA GAATTTTATT GCCTCCATTG AATTGTGGAG CAAGCACCAG
    GATAACAGAA AGCAAAAAGC TTTGGAAAAA GAACAGAGTG GTTTAAATAA ACGGAAATCA
    GGAGCGGATG GAGTAAAACA ACGTGTAGAA GGAGGAGACA CTGAGGAGAA GAAATCACAG
    ATCAGTGCAG ACAGTGGCCT CAGTGTGACA TCTGGCTCAC AGAAGAGTGA TACGGATTCC
    CTGGCGAGCT CTGAACCTCC TGCTCTGACC AGAAGCACCA GCCAGGACTC AGAGGCCAGC
    ACAGTGGTAA GTAACAGCTC AGGTGAGACG CTCGGTGCAG ACAGTGACCT GAGCAGCACT
    GCTGGAGATG GCCTGACAGG AAGACATGGT CAGCATCTCA ACCTGTCCCG TGGCACACTG
    TCTGACAGTG AGATTGAGAC CAACCCTGCA ACCAGCTCAG TGTTTGGCAA GACACACAAG
    CTGAAGCCAG GAGTGAAGGA GCCTGTTGGT GTTAATAAAG CAGCACCTGC TCCACCCGTT
    GAGGATGTTA GCATGAGGAT CTATCTGTGT GAGGGGCTTC TGGGCCGGGA TAAAAGCTCA
    GTTTGGGACC AGTTGGAGGA TGCTGCAATG GAAACCTTTT CTCTGAGTAA AGAGCGCTCA
    ACTCTGTGGG ATCAGATGCA GTTCTGGGAG GACGCCTTCC TGGATGCCGT AATGCTGGAA
    CGGGAAGGAA TGGGAATGGA TCAGGGACCA CAAGAAATGA TTGACAGGTA TCTTTCTCTG
    GGAGATCATG ACCGCAAGAG ACTGGAGGAT GATGAGGACA GACTGCTGGC CACGCTTCTG
    CACAATATGA TTGCTTACAT GCTTATGATG AAGGTCGGTA AAAACGACAT CAGGAAGAAA
    GTACGTCGTC TTATGGGCAA ATCCCACATT GGCCTCACTC ACAGTCAGGA GATCAATGAA
    GTCCTGGATC GCCTCGCACA TTTAAGCGGT CGGGAGCTTT CTATCAGGCC GAGTGGCAGC
    AGGCATATTA AAAAACAGAC ATTTGTTGTG CATGCTGGGA CAGATACAAC TGGTGACATA
    TTCTTCATGG AGGTGTGTGA TGATTGCATC GTCTTGCGCA GTAATATTGG CACAGTCTAT
    GAGCGCTGGT GGTATGAGAA GCTCATAAAT ATGACTTACT GTCCCAAAAC TAAAGTGTTG
    TGTCTGTGGA GGAGAAACGG CCAGGAGACG CAGCTAAACA AGTTTTACAC TAAGAAGTGT
    CGGGAATTGT ACTACTGTGT TAAAGACAGC ATGGAGCGTG CTGCTGCTCG ACAACAAAGC
    ATCAAACCAG GTCCAGAGCT TGGAGGCGAG TTTCCGGTTC AGGACATGAA GACAGGGGAG
    GGTGGTCTCC TCCAAGTCAC ACTGGAAGGC ATCAACCTCA AATTTATGCA CAGCCAGGAG
    CGGAAGGTTT TCATAGAGCT GAATCACATT AAAAAGTGCA ATACAGTGAA AGGAGTCTTT
    GTCCTGGAGG AGTTTGTTCC TGAAACTAAA GAAGTGGTGA TCCATAAGTA CAAGACTCCT
    ATGGCACATC AGATCTGTTA CTCGGTGCTC TGCCTCTTCT CCTACATGGC GGCGGTCAAG
    GGGAAAGAGT CAGAGGGCAA ACCCAAGATG CTGTCCCCTC GTCCACTAGC GAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333558.1
    CDS432..5528
    Translation

    Target ORF information:

    RefSeq Version XM_021477883.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021477883.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGGCT
    CTGGCTAGCA TGAGTCTAAA CACACAGCCT ATTCTGAACC TGGAGAAGTT TCAGGATGGA
    CAGGAGTTGT CTTTATTGCC CCCCGGTCGT GACAAAGCTT CCCCGTCCTC CACTGAGTTC
    AACCCACTCA TCTACGGCAA TGATGTGGAC TCAGTTGATG TTGCCACAAG GGTGGCAATG
    GTGAGGTTCT TCAACTCTCC AAACGTGCTG CAAGGGTTCC AGATGCACAC ACGCACTCTC
    CGTCTCTTCC CTCGTCCTGT GGTGGCCTTT CAGTCCACAT CTTTCCTGGC TTCTAGGCCA
    CGACGAAATG GATTCACAGA AAAGCTCTCC CACACTCAGG CAGTGGAGTA CTATGGAGAA
    TGGGCTCTCA ACCCCACCAA CCTGGCTTTC CAAAGGATTC ATAACAATGT GTATGACCCC
    TCTCTAATAG GCGATAAGCC TAAGTGGTAT GCCCACCAGT TACAGCCAGT ATTCTACCGC
    GTGTATGATG GAAACTCTCA TTTAGCGGAG GCCTTGAGTG GACCCCTGCA GGATGAGACC
    AATGATTCAG ACCCAACAGA CGACAGTGGC AGCGACAGTG AAGCATATGA TGACTCCAGC
    TCCTCCTACT CATCTCTTGG AGATTTTGTG AATGAGATGA TCAAAGGGGA CATACAGGGA
    GACACACCAA ATGTTGATCC CCTGACCCAT GCTGCACTGG GAGATGCAGA TGAGGTTGAG
    ATCCATGACT TTCACGAGTA TAAGGGGAAC AGCGGTGAAC CAGAGCCAGA AGGCCCCACA
    GAAGCAGCGG ACAGTCAGCC CTTGCGGTCC AGCTCTAGCA CCACAGCCAG TTCCAGCCCC
    AGTACAGTCA TACAGGGAGT CAACCATGAG CAGAAGGAAC CTGTTGAGGT GGAGCCCATA
    GCAAATGTGA CTTACCCAAG CTCTGCCCCA GTATTGGGTC CTCCTCCTTT TACAAGACCC
    ACCCCTGATC CAACCCTTGT TGACCCTGCC AATAAGAAGC GAGAATATGA CAACCCCTAT
    TTTGAGCCCC AGTACGGTTT CCCTACAGAG GAAGATGCTG AGACTGATGA GCAGGAGGAG
    AGTTACACAC CACGATTTAA CCAGAATCTC AATGGCAATA AGCCTTCTCG TCCACTGAGA
    CCCAGCAGCC TGAAGCTTCC GGGTGAATCT GACGGAGAGG GAGACTCCAG AAATAGCTCT
    CCTAACTCAA CCATCTCCAA CAATAGCAGT GATGGCTTTG GGGGACTCAT GTCCTTTGCC
    AGTAACTTGT ATAAAAACCA TGGCACCAGT TTCAGTCTGT CCAGCCTGGC TCTGCCTAAT
    AAAGCGAGAG AGAAGAACAC GCCGTTTCCC AGCCTCAAAG AATATTTTAA TTTTGATTTA
    GCAGAGGACA CAGATCAAGA CGATGCTGAT GACAGTCCTG AAAGTCCTGT ATTTGGGCTA
    AATTCTCTAA TGGAGATTGT AACAGAGATC GGCCCGGGGA GCGGAGAAGG TGCCCGAGCC
    CCCAGAGCAC TGGTAGATCA GAAATCCTCT GTTATTAAGC ACAGCCCTAC TGTGAAGAGA
    GAGTCTCCGT CTCCTCAAGG CAGAGCCAAC AATACCAGTG AGAACCAGCA GTTTCTAAAG
    GAGGTGGTCC AGAGCGTGCT GGAGGGGCAG GGTGTGGGCT GGCTTAACAT GAAGAAGGTG
    CGTCGCCTGC TAGAGAATGA ACAGCTCAGA GTCTTTGTGC TCAGCAAACT CAACCGTGCG
    GTCCAGTCAG AGGAGGACGC CCAGCAGGAA GTTATAAGAG ATGTGGAGAT AAATAGAAAG
    GTCTACAAAG GCATGCTGGA TATTTTGAAG TGCACGGTGT CCAGTTTGGA GCATTCATAC
    ACTAATGCTG GCTTAGGGGG CATGGCCAGT GTCTTCAGTC TGCTAGAGAT AGCACGCACC
    CACTACCAAA CCAAAGATCC AGAGAAGCGG AAACGGAGCC CCACCGAAGG GCTTAGCAGT
    CCAGGCAGTA AGGAGAGCCC ATCGGGCCGA ATGGAGAGTG CCAGGGCGGC AGGGGTGCTT
    CTGGTACCCC GTATCCAATT GCCGCCACCC TCCTCTGGGA AATCATCACG CCAGTTTGAT
    ACCCGGAGTC TGAACGAGGA GAATTTTATT GCCTCCATTG AATTGTGGAG CAAGCACCAG
    GATAACAGAA AGCAAAAAGC TTTGGAAAAA GAACAGAGTG GTTTAAATAA ACGGAAATCA
    GGAGCGGATG GAGTAAAACA ACGTGTAGAA GGAGGAGACA CTGAGGAGAA GAAATCACAG
    ATCAGTGCAG ACAGTGGCCT CAGTGTGACA TCTGGCTCAC AGAAGAGTGA TACGGATTCC
    CTGGCGAGCT CTGAACCTCC TGCTCTGACC AGAAGCACCA GCCAGGACTC AGAGGCCAGC
    ACAGTGGTAA GTAACAGCTC AGGTGAGACG CTCGGTGCAG ACAGTGACCT GAGCAGCACT
    GCTGGAGATG GCCTGACAGG AAGACATGGT CAGCATCTCA ACCTGTCCCG TGGCACACTG
    TCTGACAGTG AGATTGAGAC CAACCCTGCA ACCAGCTCAG TGTTTGGCAA GACACACAAG
    CTGAAGCCAG GAGTGAAGGA GCCTGTTGGT GTTAATAAAG CAGCACCTGC TCCACCCGTT
    GAGGATGTTA GCATGAGGAT CTATCTGTGT GAGGGGCTTC TGGGCCGGGA TAAAAGCTCA
    GTTTGGGACC AGTTGGAGGA TGCTGCAATG GAAACCTTTT CTCTGAGTAA AGAGCGCTCA
    ACTCTGTGGG ATCAGATGCA GTTCTGGGAG GACGCCTTCC TGGATGCCGT AATGCTGGAA
    CGGGAAGGAA TGGGAATGGA TCAGGGACCA CAAGAAATGA TTGACAGGTA TCTTTCTCTG
    GGAGATCATG ACCGCAAGAG ACTGGAGGAT GATGAGGACA GACTGCTGGC CACGCTTCTG
    CACAATATGA TTGCTTACAT GCTTATGATG AAGGTCGGTA AAAACGACAT CAGGAAGAAA
    GTACGTCGTC TTATGGGCAA ATCCCACATT GGCCTCACTC ACAGTCAGGA GATCAATGAA
    GTCCTGGATC GCCTCGCACA TTTAAGCGGT CGGGAGCTTT CTATCAGGCC GAGTGGCAGC
    AGGCATATTA AAAAACAGAC ATTTGTTGTG CATGCTGGGA CAGATACAAC TGGTGACATA
    TTCTTCATGG AGGTGTGTGA TGATTGCATC GTCTTGCGCA GTAATATTGG CACAGTCTAT
    GAGCGCTGGT GGTATGAGAA GCTCATAAAT ATGACTTACT GTCCCAAAAC TAAAGTGTTG
    TGTCTGTGGA GGAGAAACGG CCAGGAGACG CAGCTAAACA AGTTTTACAC TAAGAAGTGT
    CGGGAATTGT ACTACTGTGT TAAAGACAGC ATGGAGCGTG CTGCTGCTCG ACAACAAAGC
    ATCAAACCAG GTCCAGAGCT TGGAGGCGAG TTTCCGGTTC AGGACATGAA GACAGGGGAG
    GGTGGTCTCC TCCAAGTCAC ACTGGAAGGC ATCAACCTCA AATTTATGCA CAGCCAGGAG
    CGGAAGGTTT TCATAGAGCT GAATCACATT AAAAAGTGCA ATACAGTGAA AGGAGTCTTT
    GTCCTGGAGG AGTTTGTTCC TGAAACTAAA GAAGTGGTGA TCCATAAGTA CAAGACTCCT
    ATGGCACATC AGATCTGTTA CTCGGTGCTC TGCCTCTTCT CCTACATGGC GGCGGTCAAG
    GGGAAAGAGT CAGAGGGCAA ACCCAAGATG CTGTCCCCTC GTCCACTAGC GAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa143473
    Clone ID Related Accession (Same CDS sequence) XM_021477884.1
    Accession Version XM_021477884.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5094bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA TTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGAACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCAGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT ACCCAAGCTC TGCCCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTTCCCAGC CTCAAAGAAT ATTTTAATTT TGATTTAGCA
    GAGGACACAG ATCAAGACGA TGCTGATGAC AGTCCTGAAA GTCCTGGTGC CCGAGCCCCC
    AGAGCACTGG TAGATCAGAA ATCCTCTGTT ATTAAGCACA GCCCTACTGT GAAGAGAGAG
    TCTCCGTCTC CTCAAGGCAG AGCCAACAAT ACCAGTGAGA ACCAGCAGTT TCTAAAGGAG
    GTGGTCCAGA GCGTGCTGGA GGGGCAGGGT GTGGGCTGGC TTAACATGAA GAAGGTGCGT
    CGCCTGCTAG AGAATGAACA GCTCAGAGTC TTTGTGCTCA GCAAACTCAA CCGTGCGGTC
    CAGTCAGAGG AGGACGCCCA GCAGGAAGTT ATAAGAGATG TGGAGATAAA TAGAAAGGTC
    TACAAAGGCA TGCTGGATAT TTTGAAGTGC ACGGTGTCCA GTTTGGAGCA TTCATACACT
    AATGCTGGCT TAGGGGGCAT GGCCAGTGTC TTCAGTCTGC TAGAGATAGC ACGCACCCAC
    TACCAAACCA AAGATCCAGA GAAGCGGAAA CGGAGCCCCA CCGAAGGGCT TAGCAGTCCA
    GGCAGTAAGG AGAGCCCATC GGGCCGAATG GAGAGTGCCA GGGCGGCAGG GGTGCTTCTG
    GTACCCCGTA TCCAATTGCC GCCACCCTCC TCTGGGAAAT CATCACGCCA GTTTGATACC
    CGGAGTCTGA ACGAGGAGAA TTTTATTGCC TCCATTGAAT TGTGGAGCAA GCACCAGGAT
    AACAGAAAGC AAAAAGCTTT GGAAAAAGAA CAGAGTGGTT TAAATAAACG GAAATCAGGA
    GCGGATGGAG TAAAACAACG TGTAGAAGGA GGAGACACTG AGGAGAAGAA ATCACAGATC
    AGTGCAGACA GTGGCCTCAG TGTGACATCT GGCTCACAGA AGAGTGATAC GGATTCCCTG
    GCGAGCTCTG AACCTCCTGC TCTGACCAGA AGCACCAGCC AGGACTCAGA GGCCAGCACA
    GTGGTAAGTA ACAGCTCAGG TGAGACGCTC GGTGCAGACA GTGACCTGAG CAGCACTGCT
    GGAGATGGCC TGACAGGAAG ACATGGTCAG CATCTCAACC TGTCCCGTGG CACACTGTCT
    GACAGTGAGA TTGAGACCAA CCCTGCAACC AGCTCAGTGT TTGGCAAGAC ACACAAGCTG
    AAGCCAGGAG TGAAGGAGCC TGTTGGTGTT AATAAAGCAG CACCTGCTCC ACCCGTTGAG
    GATGTTAGCA TGAGGATCTA TCTGTGTGAG GGGCTTCTGG GCCGGGATAA AAGCTCAGTT
    TGGGACCAGT TGGAGGATGC TGCAATGGAA ACCTTTTCTC TGAGTAAAGA GCGCTCAACT
    CTGTGGGATC AGATGCAGTT CTGGGAGGAC GCCTTCCTGG ATGCCGTAAT GCTGGAACGG
    GAAGGAATGG GAATGGATCA GGGACCACAA GAAATGATTG ACAGGTATCT TTCTCTGGGA
    GATCATGACC GCAAGAGACT GGAGGATGAT GAGGACAGAC TGCTGGCCAC GCTTCTGCAC
    AATATGATTG CTTACATGCT TATGATGAAG GTCGGTAAAA ACGACATCAG GAAGAAAGTA
    CGTCGTCTTA TGGGCAAATC CCACATTGGC CTCACTCACA GTCAGGAGAT CAATGAAGTC
    CTGGATCGCC TCGCACATTT AAGCGGTCGG GAGCTTTCTA TCAGGCCGAG TGGCAGCAGG
    CATATTAAAA AACAGACATT TGTTGTGCAT GCTGGGACAG ATACAACTGG TGACATATTC
    TTCATGGAGG TGTGTGATGA TTGCATCGTC TTGCGCAGTA ATATTGGCAC AGTCTATGAG
    CGCTGGTGGT ATGAGAAGCT CATAAATATG ACTTACTGTC CCAAAACTAA AGTGTTGTGT
    CTGTGGAGGA GAAACGGCCA GGAGACGCAG CTAAACAAGT TTTACACTAA GAAGTGTCGG
    GAATTGTACT ACTGTGTTAA AGACAGCATG GAGCGTGCTG CTGCTCGACA ACAAAGCATC
    AAACCAGGTC CAGAGCTTGG AGGCGAGTTT CCGGTTCAGG ACATGAAGAC AGGGGAGGGT
    GGTCTCCTCC AAGTCACACT GGAAGGCATC AACCTCAAAT TTATGCACAG CCAGGAGCGG
    AAGGTTTTCA TAGAGCTGAA TCACATTAAA AAGTGCAATA CAGTGAAAGG AGTCTTTGTC
    CTGGAGGAGT TTGTTCCTGA AACTAAAGAA GTGGTGATCC ATAAGTACAA GACTCCTATG
    GCACATCAGA TCTGTTACTC GGTGCTCTGC CTCTTCTCCT ACATGGCGGC GGTCAAGGGG
    AAAGAGTCAG AGGGCAAACC CAAGATGCTG TCCCCTCGTC CACTAGCGAG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333559.1
    CDS432..5525
    Translation

    Target ORF information:

    RefSeq Version XM_021477884.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021477884.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA TTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGAACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCAGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT ACCCAAGCTC TGCCCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTTCCCAGC CTCAAAGAAT ATTTTAATTT TGATTTAGCA
    GAGGACACAG ATCAAGACGA TGCTGATGAC AGTCCTGAAA GTCCTGGTGC CCGAGCCCCC
    AGAGCACTGG TAGATCAGAA ATCCTCTGTT ATTAAGCACA GCCCTACTGT GAAGAGAGAG
    TCTCCGTCTC CTCAAGGCAG AGCCAACAAT ACCAGTGAGA ACCAGCAGTT TCTAAAGGAG
    GTGGTCCAGA GCGTGCTGGA GGGGCAGGGT GTGGGCTGGC TTAACATGAA GAAGGTGCGT
    CGCCTGCTAG AGAATGAACA GCTCAGAGTC TTTGTGCTCA GCAAACTCAA CCGTGCGGTC
    CAGTCAGAGG AGGACGCCCA GCAGGAAGTT ATAAGAGATG TGGAGATAAA TAGAAAGGTC
    TACAAAGGCA TGCTGGATAT TTTGAAGTGC ACGGTGTCCA GTTTGGAGCA TTCATACACT
    AATGCTGGCT TAGGGGGCAT GGCCAGTGTC TTCAGTCTGC TAGAGATAGC ACGCACCCAC
    TACCAAACCA AAGATCCAGA GAAGCGGAAA CGGAGCCCCA CCGAAGGGCT TAGCAGTCCA
    GGCAGTAAGG AGAGCCCATC GGGCCGAATG GAGAGTGCCA GGGCGGCAGG GGTGCTTCTG
    GTACCCCGTA TCCAATTGCC GCCACCCTCC TCTGGGAAAT CATCACGCCA GTTTGATACC
    CGGAGTCTGA ACGAGGAGAA TTTTATTGCC TCCATTGAAT TGTGGAGCAA GCACCAGGAT
    AACAGAAAGC AAAAAGCTTT GGAAAAAGAA CAGAGTGGTT TAAATAAACG GAAATCAGGA
    GCGGATGGAG TAAAACAACG TGTAGAAGGA GGAGACACTG AGGAGAAGAA ATCACAGATC
    AGTGCAGACA GTGGCCTCAG TGTGACATCT GGCTCACAGA AGAGTGATAC GGATTCCCTG
    GCGAGCTCTG AACCTCCTGC TCTGACCAGA AGCACCAGCC AGGACTCAGA GGCCAGCACA
    GTGGTAAGTA ACAGCTCAGG TGAGACGCTC GGTGCAGACA GTGACCTGAG CAGCACTGCT
    GGAGATGGCC TGACAGGAAG ACATGGTCAG CATCTCAACC TGTCCCGTGG CACACTGTCT
    GACAGTGAGA TTGAGACCAA CCCTGCAACC AGCTCAGTGT TTGGCAAGAC ACACAAGCTG
    AAGCCAGGAG TGAAGGAGCC TGTTGGTGTT AATAAAGCAG CACCTGCTCC ACCCGTTGAG
    GATGTTAGCA TGAGGATCTA TCTGTGTGAG GGGCTTCTGG GCCGGGATAA AAGCTCAGTT
    TGGGACCAGT TGGAGGATGC TGCAATGGAA ACCTTTTCTC TGAGTAAAGA GCGCTCAACT
    CTGTGGGATC AGATGCAGTT CTGGGAGGAC GCCTTCCTGG ATGCCGTAAT GCTGGAACGG
    GAAGGAATGG GAATGGATCA GGGACCACAA GAAATGATTG ACAGGTATCT TTCTCTGGGA
    GATCATGACC GCAAGAGACT GGAGGATGAT GAGGACAGAC TGCTGGCCAC GCTTCTGCAC
    AATATGATTG CTTACATGCT TATGATGAAG GTCGGTAAAA ACGACATCAG GAAGAAAGTA
    CGTCGTCTTA TGGGCAAATC CCACATTGGC CTCACTCACA GTCAGGAGAT CAATGAAGTC
    CTGGATCGCC TCGCACATTT AAGCGGTCGG GAGCTTTCTA TCAGGCCGAG TGGCAGCAGG
    CATATTAAAA AACAGACATT TGTTGTGCAT GCTGGGACAG ATACAACTGG TGACATATTC
    TTCATGGAGG TGTGTGATGA TTGCATCGTC TTGCGCAGTA ATATTGGCAC AGTCTATGAG
    CGCTGGTGGT ATGAGAAGCT CATAAATATG ACTTACTGTC CCAAAACTAA AGTGTTGTGT
    CTGTGGAGGA GAAACGGCCA GGAGACGCAG CTAAACAAGT TTTACACTAA GAAGTGTCGG
    GAATTGTACT ACTGTGTTAA AGACAGCATG GAGCGTGCTG CTGCTCGACA ACAAAGCATC
    AAACCAGGTC CAGAGCTTGG AGGCGAGTTT CCGGTTCAGG ACATGAAGAC AGGGGAGGGT
    GGTCTCCTCC AAGTCACACT GGAAGGCATC AACCTCAAAT TTATGCACAG CCAGGAGCGG
    AAGGTTTTCA TAGAGCTGAA TCACATTAAA AAGTGCAATA CAGTGAAAGG AGTCTTTGTC
    CTGGAGGAGT TTGTTCCTGA AACTAAAGAA GTGGTGATCC ATAAGTACAA GACTCCTATG
    GCACATCAGA TCTGTTACTC GGTGCTCTGC CTCTTCTCCT ACATGGCGGC GGTCAAGGGG
    AAAGAGTCAG AGGGCAAACC CAAGATGCTG TCCCCTCGTC CACTAGCGAG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa143474
    Clone ID Related Accession (Same CDS sequence) XM_021477885.1
    Accession Version XM_021477885.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5091bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA TTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGAACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCAGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT ACCCAAGCTC TGCCCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTTCCCAGC CTCAAAGAAT ATTTTAATTT TGATTTAGCA
    GAGGACACAG ATCAAGACGA TGCTGATGAC AGTCCTGAAA GTCCTGTATT TGGGCTAAAT
    TCTCTAATGG AGATTGTAAC AGAGATCGGC CCGGGGAGCG GAGAAGGTGC CCGAGCCCCC
    AGAGCACTGG TAGATCAGAA ATCCTCTGTT ATTAAGCACA GCCCTACTGT GAAGAGAGAG
    TCTCCGTCTC CTCAAGGCAG AGCCAACAAT ACCAGTGAGA ACCAGCAGTT TCTAAAGGAG
    GTGGTCCAGA GCGTGCTGGA GGGGCAGGGT GTGGGCTGGC TTAACATGAA GAAGGTGCGT
    CGCCTGCTAG AGAATGAACA GCTCAGAGTC TTTGTGCTCA GCAAACTCAA CCGTGCGGTC
    CAGTCAGAGG AGGACGCCCA GCAGGAAGTT ATAAGAGATG TGGAGATAAA TAGAAAGGTC
    TACAAAGGCA TGCTGGATAT TTTGAAGTGC ACGGTGTCCA GTTTGGAGCA TTCATACACT
    AATGCTGGCT TAGGGGGCAT GGCCAGTGTC TTCAGTCTGC TAGAGATAGC ACGCACCCAC
    TACCAAACCA AAGATCCAGA GAAGCGGAAA CGGAGCCCCA CCGAAGGGCT TAGCAGTCCA
    GGCAGTAAGG AGAGCCCATC GGGCCGAATG GAGAGTGCCA GGGCGGCAGG GGTGCTTCTG
    GTACCCCGTA TCCAATTGCC GCCACCCTCC TCTGGGAAAT CATCACGCCA GTTTGATACC
    CGGAGTCTGA ACGAGGAGAA TTTTATTGCC TCCATTGAAT TGTGGAGCAA GCACCAGGAT
    AACAGAAAGC AAAAAGCTTT GGAAAAAGAA CAGAGTGGTT TAAATAAACG GAAATCAGGA
    GCGGATGGAG TAAAACAACG TGTAGAAGGA GGAGACACTG AGGAGAAGAA ATCACAGATC
    AGTGCAGACA GTGGCCTCAG TGTGACATCT GGCTCACAGA AGAGTGATAC GGATTCCCTG
    GCGAGCTCTG AACCTCCTGC TCTGACCAGA AGCACCAGCC AGGACTCAGA GGCCAGCACA
    GTGGTAAGTA ACAGCTCAGG TGAGACGCTC GGTGCAGACA GTGACCTGAG CAGCACTGCT
    GGAGATGGCC TGACAGGAAG ACATGGTCAG CATCTCAACC TGTCCCGTGG CACACTGTCT
    GACAGTGAGA TTGAGACCAA CCCTGCAACC AGCTCAGTGT TTGGCAAGAC ACACAAGCTG
    AAGCCAGGAG TGAAGGAGCC TGTTGGTGTT AATAAAGCAG CACCTGCTCC ACCCGTTGAG
    GATGTTAGCA TGAGGATCTA TCTGTGTGAG GGGCTTCTGG GTAAAGAGCG CTCAACTCTG
    TGGGATCAGA TGCAGTTCTG GGAGGACGCC TTCCTGGATG CCGTAATGCT GGAACGGGAA
    GGAATGGGAA TGGATCAGGG ACCACAAGAA ATGATTGACA GGTATCTTTC TCTGGGAGAT
    CATGACCGCA AGAGACTGGA GGATGATGAG GACAGACTGC TGGCCACGCT TCTGCACAAT
    ATGATTGCTT ACATGCTTAT GATGAAGGTC GGTAAAAACG ACATCAGGAA GAAAGTACGT
    CGTCTTATGG GCAAATCCCA CATTGGCCTC ACTCACAGTC AGGAGATCAA TGAAGTCCTG
    GATCGCCTCG CACATTTAAG CGGTCGGGAG CTTTCTATCA GGCCGAGTGG CAGCAGGCAT
    ATTAAAAAAC AGACATTTGT TGTGCATGCT GGGACAGATA CAACTGGTGA CATATTCTTC
    ATGGAGGTGT GTGATGATTG CATCGTCTTG CGCAGTAATA TTGGCACAGT CTATGAGCGC
    TGGTGGTATG AGAAGCTCAT AAATATGACT TACTGTCCCA AAACTAAAGT GTTGTGTCTG
    TGGAGGAGAA ACGGCCAGGA GACGCAGCTA AACAAGTTTT ACACTAAGAA GTGTCGGGAA
    TTGTACTACT GTGTTAAAGA CAGCATGGAG CGTGCTGCTG CTCGACAACA AAGCATCAAA
    CCAGGTCCAG AGCTTGGAGG CGAGTTTCCG GTTCAGGACA TGAAGACAGG GGAGGGTGGT
    CTCCTCCAAG TCACACTGGA AGGCATCAAC CTCAAATTTA TGCACAGCCA GGAGCGGAAG
    GTTTTCATAG AGCTGAATCA CATTAAAAAG TGCAATACAG TGAAAGGAGT CTTTGTCCTG
    GAGGAGTTTG TTCCTGAAAC TAAAGAAGTG GTGATCCATA AGTACAAGAC TCCTATGGCA
    CATCAGATCT GTTACTCGGT GCTCTGCCTC TTCTCCTACA TGGCGGCGGT CAAGGGGAAA
    GAGTCAGAGG GCAAACCCAA GATGCTGTCC CCTCGTCCAC TAGCGAGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333560.1
    CDS432..5522
    Translation

    Target ORF information:

    RefSeq Version XM_021477885.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021477885.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA TTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGAACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCAGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT ACCCAAGCTC TGCCCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTTCCCAGC CTCAAAGAAT ATTTTAATTT TGATTTAGCA
    GAGGACACAG ATCAAGACGA TGCTGATGAC AGTCCTGAAA GTCCTGTATT TGGGCTAAAT
    TCTCTAATGG AGATTGTAAC AGAGATCGGC CCGGGGAGCG GAGAAGGTGC CCGAGCCCCC
    AGAGCACTGG TAGATCAGAA ATCCTCTGTT ATTAAGCACA GCCCTACTGT GAAGAGAGAG
    TCTCCGTCTC CTCAAGGCAG AGCCAACAAT ACCAGTGAGA ACCAGCAGTT TCTAAAGGAG
    GTGGTCCAGA GCGTGCTGGA GGGGCAGGGT GTGGGCTGGC TTAACATGAA GAAGGTGCGT
    CGCCTGCTAG AGAATGAACA GCTCAGAGTC TTTGTGCTCA GCAAACTCAA CCGTGCGGTC
    CAGTCAGAGG AGGACGCCCA GCAGGAAGTT ATAAGAGATG TGGAGATAAA TAGAAAGGTC
    TACAAAGGCA TGCTGGATAT TTTGAAGTGC ACGGTGTCCA GTTTGGAGCA TTCATACACT
    AATGCTGGCT TAGGGGGCAT GGCCAGTGTC TTCAGTCTGC TAGAGATAGC ACGCACCCAC
    TACCAAACCA AAGATCCAGA GAAGCGGAAA CGGAGCCCCA CCGAAGGGCT TAGCAGTCCA
    GGCAGTAAGG AGAGCCCATC GGGCCGAATG GAGAGTGCCA GGGCGGCAGG GGTGCTTCTG
    GTACCCCGTA TCCAATTGCC GCCACCCTCC TCTGGGAAAT CATCACGCCA GTTTGATACC
    CGGAGTCTGA ACGAGGAGAA TTTTATTGCC TCCATTGAAT TGTGGAGCAA GCACCAGGAT
    AACAGAAAGC AAAAAGCTTT GGAAAAAGAA CAGAGTGGTT TAAATAAACG GAAATCAGGA
    GCGGATGGAG TAAAACAACG TGTAGAAGGA GGAGACACTG AGGAGAAGAA ATCACAGATC
    AGTGCAGACA GTGGCCTCAG TGTGACATCT GGCTCACAGA AGAGTGATAC GGATTCCCTG
    GCGAGCTCTG AACCTCCTGC TCTGACCAGA AGCACCAGCC AGGACTCAGA GGCCAGCACA
    GTGGTAAGTA ACAGCTCAGG TGAGACGCTC GGTGCAGACA GTGACCTGAG CAGCACTGCT
    GGAGATGGCC TGACAGGAAG ACATGGTCAG CATCTCAACC TGTCCCGTGG CACACTGTCT
    GACAGTGAGA TTGAGACCAA CCCTGCAACC AGCTCAGTGT TTGGCAAGAC ACACAAGCTG
    AAGCCAGGAG TGAAGGAGCC TGTTGGTGTT AATAAAGCAG CACCTGCTCC ACCCGTTGAG
    GATGTTAGCA TGAGGATCTA TCTGTGTGAG GGGCTTCTGG GTAAAGAGCG CTCAACTCTG
    TGGGATCAGA TGCAGTTCTG GGAGGACGCC TTCCTGGATG CCGTAATGCT GGAACGGGAA
    GGAATGGGAA TGGATCAGGG ACCACAAGAA ATGATTGACA GGTATCTTTC TCTGGGAGAT
    CATGACCGCA AGAGACTGGA GGATGATGAG GACAGACTGC TGGCCACGCT TCTGCACAAT
    ATGATTGCTT ACATGCTTAT GATGAAGGTC GGTAAAAACG ACATCAGGAA GAAAGTACGT
    CGTCTTATGG GCAAATCCCA CATTGGCCTC ACTCACAGTC AGGAGATCAA TGAAGTCCTG
    GATCGCCTCG CACATTTAAG CGGTCGGGAG CTTTCTATCA GGCCGAGTGG CAGCAGGCAT
    ATTAAAAAAC AGACATTTGT TGTGCATGCT GGGACAGATA CAACTGGTGA CATATTCTTC
    ATGGAGGTGT GTGATGATTG CATCGTCTTG CGCAGTAATA TTGGCACAGT CTATGAGCGC
    TGGTGGTATG AGAAGCTCAT AAATATGACT TACTGTCCCA AAACTAAAGT GTTGTGTCTG
    TGGAGGAGAA ACGGCCAGGA GACGCAGCTA AACAAGTTTT ACACTAAGAA GTGTCGGGAA
    TTGTACTACT GTGTTAAAGA CAGCATGGAG CGTGCTGCTG CTCGACAACA AAGCATCAAA
    CCAGGTCCAG AGCTTGGAGG CGAGTTTCCG GTTCAGGACA TGAAGACAGG GGAGGGTGGT
    CTCCTCCAAG TCACACTGGA AGGCATCAAC CTCAAATTTA TGCACAGCCA GGAGCGGAAG
    GTTTTCATAG AGCTGAATCA CATTAAAAAG TGCAATACAG TGAAAGGAGT CTTTGTCCTG
    GAGGAGTTTG TTCCTGAAAC TAAAGAAGTG GTGATCCATA AGTACAAGAC TCCTATGGCA
    CATCAGATCT GTTACTCGGT GCTCTGCCTC TTCTCCTACA TGGCGGCGGT CAAGGGGAAA
    GAGTCAGAGG GCAAACCCAA GATGCTGTCC CCTCGTCCAC TAGCGAGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa143475
    Clone ID Related Accession (Same CDS sequence) XM_021477886.1
    Accession Version XM_021477886.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5088bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GAATATTTTA ATTTTGATTT AGCAGAGGAC
    ACAGATCAAG ACGATGCTGA TGACAGTCCT GAAAGTCCTG TATTTGGGCT AAATTCTCTA
    ATGGAGATTG TAACAGAGAT CGGCCCGGGG AGCGGAGAAG GTGCCCGAGC CCCCAGAGCA
    CTGGTAGATC AGAAATCCTC TGTTATTAAG CACAGCCCTA CTGTGAAGAG AGAGTCTCCG
    TCTCCTCAAG GCAGAGCCAA CAATACCAGT GAGAACCAGC AGTTTCTAAA GGAGGTGGTC
    CAGAGCGTGC TGGAGGGGCA GGGTGTGGGC TGGCTTAACA TGAAGAAGGT GCGTCGCCTG
    CTAGAGAATG AACAGCTCAG AGTCTTTGTG CTCAGCAAAC TCAACCGTGC GGTCCAGTCA
    GAGGAGGACG CCCAGCAGGA AGTTATAAGA GATGTGGAGA TAAATAGAAA GGTCTACAAA
    GGCATGCTGG ATATTTTGAA GTGCACGGTG TCCAGTTTGG AGCATTCATA CACTAATGCT
    GGCTTAGGGG GCATGGCCAG TGTCTTCAGT CTGCTAGAGA TAGCACGCAC CCACTACCAA
    ACCAAAGATC CAGAGAAGCG GAAACGGAGC CCCACCGAAG GGCTTAGCAG TCCAGGCAGT
    AAGGAGAGCC CATCGGGCCG AATGGAGAGT GCCAGGGCGG CAGGGGTGCT TCTGGTACCC
    CGTATCCAAT TGCCGCCACC CTCCTCTGGG AAATCATCAC GCCAGTTTGA TACCCGGAGT
    CTGAACGAGG AGAATTTTAT TGCCTCCATT GAATTGTGGA GCAAGCACCA GGATAACAGA
    AAGCAAAAAG CTTTGGAAAA AGAACAGAGT GGTTTAAATA AACGGAAATC AGGAGCGGAT
    GGAGTAAAAC AACGTGTAGA AGGAGGAGAC ACTGAGGAGA AGAAATCACA GATCAGTGCA
    GACAGTGGCC TCAGTGTGAC ATCTGGCTCA CAGAAGAGTG ATACGGATTC CCTGGCGAGC
    TCTGAACCTC CTGCTCTGAC CAGAAGCACC AGCCAGGACT CAGAGGCCAG CACAGTGGTA
    AGTAACAGCT CAGGTGAGAC GCTCGGTGCA GACAGTGACC TGAGCAGCAC TGCTGGAGAT
    GGCCTGACAG GAAGACATGG TCAGCATCTC AACCTGTCCC GTGGCACACT GTCTGACAGT
    GAGATTGAGA CCAACCCTGC AACCAGCTCA GTGTTTGGCA AGACACACAA GCTGAAGCCA
    GGAGTGAAGG AGCCTGTTGG TGTTAATAAA GCAGCACCTG CTCCACCCGT TGAGGATGTT
    AGCATGAGGA TCTATCTGTG TGAGGGGCTT CTGGGCCGGG ATAAAAGCTC AGTTTGGGAC
    CAGTTGGAGG ATGCTGCAAT GGAAACCTTT TCTCTGAGTA AAGAGCGCTC AACTCTGTGG
    GATCAGATGC AGTTCTGGGA GGACGCCTTC CTGGATGCCG TAATGCTGGA ACGGGAAGGA
    ATGGGAATGG ATCAGGGACC ACAAGAAATG ATTGACAGGT ATCTTTCTCT GGGAGATCAT
    GACCGCAAGA GACTGGAGGA TGATGAGGAC AGACTGCTGG CCACGCTTCT GCACAATATG
    ATTGCTTACA TGCTTATGAT GAAGGTCGGT AAAAACGACA TCAGGAAGAA AGTACGTCGT
    CTTATGGGCA AATCCCACAT TGGCCTCACT CACAGTCAGG AGATCAATGA AGTCCTGGAT
    CGCCTCGCAC ATTTAAGCGG TCGGGAGCTT TCTATCAGGC CGAGTGGCAG CAGGCATATT
    AAAAAACAGA CATTTGTTGT GCATGCTGGG ACAGATACAA CTGGTGACAT ATTCTTCATG
    GAGGTGTGTG ATGATTGCAT CGTCTTGCGC AGTAATATTG GCACAGTCTA TGAGCGCTGG
    TGGTATGAGA AGCTCATAAA TATGACTTAC TGTCCCAAAA CTAAAGTGTT GTGTCTGTGG
    AGGAGAAACG GCCAGGAGAC GCAGCTAAAC AAGTTTTACA CTAAGAAGTG TCGGGAATTG
    TACTACTGTG TTAAAGACAG CATGGAGCGT GCTGCTGCTC GACAACAAAG CATCAAACCA
    GGTCCAGAGC TTGGAGGCGA GTTTCCGGTT CAGGACATGA AGACAGGGGA GGGTGGTCTC
    CTCCAAGTCA CACTGGAAGG CATCAACCTC AAATTTATGC ACAGCCAGGA GCGGAAGGTT
    TTCATAGAGC TGAATCACAT TAAAAAGTGC AATACAGTGA AAGGAGTCTT TGTCCTGGAG
    GAGTTTGTTC CTGAAACTAA AGAAGTGGTG ATCCATAAGT ACAAGACTCC TATGGCACAT
    CAGATCTGTT ACTCGGTGCT CTGCCTCTTC TCCTACATGG CGGCGGTCAA GGGGAAAGAG
    TCAGAGGGCA AACCCAAGAT GCTGTCCCCT CGTCCACTAG CGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333561.1
    CDS432..5519
    Translation

    Target ORF information:

    RefSeq Version XM_021477886.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021477886.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GAATATTTTA ATTTTGATTT AGCAGAGGAC
    ACAGATCAAG ACGATGCTGA TGACAGTCCT GAAAGTCCTG TATTTGGGCT AAATTCTCTA
    ATGGAGATTG TAACAGAGAT CGGCCCGGGG AGCGGAGAAG GTGCCCGAGC CCCCAGAGCA
    CTGGTAGATC AGAAATCCTC TGTTATTAAG CACAGCCCTA CTGTGAAGAG AGAGTCTCCG
    TCTCCTCAAG GCAGAGCCAA CAATACCAGT GAGAACCAGC AGTTTCTAAA GGAGGTGGTC
    CAGAGCGTGC TGGAGGGGCA GGGTGTGGGC TGGCTTAACA TGAAGAAGGT GCGTCGCCTG
    CTAGAGAATG AACAGCTCAG AGTCTTTGTG CTCAGCAAAC TCAACCGTGC GGTCCAGTCA
    GAGGAGGACG CCCAGCAGGA AGTTATAAGA GATGTGGAGA TAAATAGAAA GGTCTACAAA
    GGCATGCTGG ATATTTTGAA GTGCACGGTG TCCAGTTTGG AGCATTCATA CACTAATGCT
    GGCTTAGGGG GCATGGCCAG TGTCTTCAGT CTGCTAGAGA TAGCACGCAC CCACTACCAA
    ACCAAAGATC CAGAGAAGCG GAAACGGAGC CCCACCGAAG GGCTTAGCAG TCCAGGCAGT
    AAGGAGAGCC CATCGGGCCG AATGGAGAGT GCCAGGGCGG CAGGGGTGCT TCTGGTACCC
    CGTATCCAAT TGCCGCCACC CTCCTCTGGG AAATCATCAC GCCAGTTTGA TACCCGGAGT
    CTGAACGAGG AGAATTTTAT TGCCTCCATT GAATTGTGGA GCAAGCACCA GGATAACAGA
    AAGCAAAAAG CTTTGGAAAA AGAACAGAGT GGTTTAAATA AACGGAAATC AGGAGCGGAT
    GGAGTAAAAC AACGTGTAGA AGGAGGAGAC ACTGAGGAGA AGAAATCACA GATCAGTGCA
    GACAGTGGCC TCAGTGTGAC ATCTGGCTCA CAGAAGAGTG ATACGGATTC CCTGGCGAGC
    TCTGAACCTC CTGCTCTGAC CAGAAGCACC AGCCAGGACT CAGAGGCCAG CACAGTGGTA
    AGTAACAGCT CAGGTGAGAC GCTCGGTGCA GACAGTGACC TGAGCAGCAC TGCTGGAGAT
    GGCCTGACAG GAAGACATGG TCAGCATCTC AACCTGTCCC GTGGCACACT GTCTGACAGT
    GAGATTGAGA CCAACCCTGC AACCAGCTCA GTGTTTGGCA AGACACACAA GCTGAAGCCA
    GGAGTGAAGG AGCCTGTTGG TGTTAATAAA GCAGCACCTG CTCCACCCGT TGAGGATGTT
    AGCATGAGGA TCTATCTGTG TGAGGGGCTT CTGGGCCGGG ATAAAAGCTC AGTTTGGGAC
    CAGTTGGAGG ATGCTGCAAT GGAAACCTTT TCTCTGAGTA AAGAGCGCTC AACTCTGTGG
    GATCAGATGC AGTTCTGGGA GGACGCCTTC CTGGATGCCG TAATGCTGGA ACGGGAAGGA
    ATGGGAATGG ATCAGGGACC ACAAGAAATG ATTGACAGGT ATCTTTCTCT GGGAGATCAT
    GACCGCAAGA GACTGGAGGA TGATGAGGAC AGACTGCTGG CCACGCTTCT GCACAATATG
    ATTGCTTACA TGCTTATGAT GAAGGTCGGT AAAAACGACA TCAGGAAGAA AGTACGTCGT
    CTTATGGGCA AATCCCACAT TGGCCTCACT CACAGTCAGG AGATCAATGA AGTCCTGGAT
    CGCCTCGCAC ATTTAAGCGG TCGGGAGCTT TCTATCAGGC CGAGTGGCAG CAGGCATATT
    AAAAAACAGA CATTTGTTGT GCATGCTGGG ACAGATACAA CTGGTGACAT ATTCTTCATG
    GAGGTGTGTG ATGATTGCAT CGTCTTGCGC AGTAATATTG GCACAGTCTA TGAGCGCTGG
    TGGTATGAGA AGCTCATAAA TATGACTTAC TGTCCCAAAA CTAAAGTGTT GTGTCTGTGG
    AGGAGAAACG GCCAGGAGAC GCAGCTAAAC AAGTTTTACA CTAAGAAGTG TCGGGAATTG
    TACTACTGTG TTAAAGACAG CATGGAGCGT GCTGCTGCTC GACAACAAAG CATCAAACCA
    GGTCCAGAGC TTGGAGGCGA GTTTCCGGTT CAGGACATGA AGACAGGGGA GGGTGGTCTC
    CTCCAAGTCA CACTGGAAGG CATCAACCTC AAATTTATGC ACAGCCAGGA GCGGAAGGTT
    TTCATAGAGC TGAATCACAT TAAAAAGTGC AATACAGTGA AAGGAGTCTT TGTCCTGGAG
    GAGTTTGTTC CTGAAACTAA AGAAGTGGTG ATCCATAAGT ACAAGACTCC TATGGCACAT
    CAGATCTGTT ACTCGGTGCT CTGCCTCTTC TCCTACATGG CGGCGGTCAA GGGGAAAGAG
    TCAGAGGGCA AACCCAAGAT GCTGTCCCCT CGTCCACTAG CGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa143476
    Clone ID Related Accession (Same CDS sequence) XM_021477887.1
    Accession Version XM_021477887.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5085bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA TTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGAACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCAGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT ACCCAAGCTC TGCCCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTTCCCAGC CTCAAAGTAT TTGGGCTAAA TTCTCTAATG
    GAGATTGTAA CAGAGATCGG CCCGGGGAGC GGAGAAGGTG CCCGAGCCCC CAGAGCACTG
    GTAGATCAGA AATCCTCTGT TATTAAGCAC AGCCCTACTG TGAAGAGAGA GTCTCCGTCT
    CCTCAAGGCA GAGCCAACAA TACCAGTGAG AACCAGCAGT TTCTAAAGGA GGTGGTCCAG
    AGCGTGCTGG AGGGGCAGGG TGTGGGCTGG CTTAACATGA AGAAGGTGCG TCGCCTGCTA
    GAGAATGAAC AGCTCAGAGT CTTTGTGCTC AGCAAACTCA ACCGTGCGGT CCAGTCAGAG
    GAGGACGCCC AGCAGGAAGT TATAAGAGAT GTGGAGATAA ATAGAAAGGT CTACAAAGGC
    ATGCTGGATA TTTTGAAGTG CACGGTGTCC AGTTTGGAGC ATTCATACAC TAATGCTGGC
    TTAGGGGGCA TGGCCAGTGT CTTCAGTCTG CTAGAGATAG CACGCACCCA CTACCAAACC
    AAAGATCCAG AGAAGCGGAA ACGGAGCCCC ACCGAAGGGC TTAGCAGTCC AGGCAGTAAG
    GAGAGCCCAT CGGGCCGAAT GGAGAGTGCC AGGGCGGCAG GGGTGCTTCT GGTACCCCGT
    ATCCAATTGC CGCCACCCTC CTCTGGGAAA TCATCACGCC AGTTTGATAC CCGGAGTCTG
    AACGAGGAGA ATTTTATTGC CTCCATTGAA TTGTGGAGCA AGCACCAGGA TAACAGAAAG
    CAAAAAGCTT TGGAAAAAGA ACAGAGTGGT TTAAATAAAC GGAAATCAGG AGCGGATGGA
    GTAAAACAAC GTGTAGAAGG AGGAGACACT GAGGAGAAGA AATCACAGAT CAGTGCAGAC
    AGTGGCCTCA GTGTGACATC TGGCTCACAG AAGAGTGATA CGGATTCCCT GGCGAGCTCT
    GAACCTCCTG CTCTGACCAG AAGCACCAGC CAGGACTCAG AGGCCAGCAC AGTGGTAAGT
    AACAGCTCAG GTGAGACGCT CGGTGCAGAC AGTGACCTGA GCAGCACTGC TGGAGATGGC
    CTGACAGGAA GACATGGTCA GCATCTCAAC CTGTCCCGTG GCACACTGTC TGACAGTGAG
    ATTGAGACCA ACCCTGCAAC CAGCTCAGTG TTTGGCAAGA CACACAAGCT GAAGCCAGGA
    GTGAAGGAGC CTGTTGGTGT TAATAAAGCA GCACCTGCTC CACCCGTTGA GGATGTTAGC
    ATGAGGATCT ATCTGTGTGA GGGGCTTCTG GGCCGGGATA AAAGCTCAGT TTGGGACCAG
    TTGGAGGATG CTGCAATGGA AACCTTTTCT CTGAGTAAAG AGCGCTCAAC TCTGTGGGAT
    CAGATGCAGT TCTGGGAGGA CGCCTTCCTG GATGCCGTAA TGCTGGAACG GGAAGGAATG
    GGAATGGATC AGGGACCACA AGAAATGATT GACAGGTATC TTTCTCTGGG AGATCATGAC
    CGCAAGAGAC TGGAGGATGA TGAGGACAGA CTGCTGGCCA CGCTTCTGCA CAATATGATT
    GCTTACATGC TTATGATGAA GGTCGGTAAA AACGACATCA GGAAGAAAGT ACGTCGTCTT
    ATGGGCAAAT CCCACATTGG CCTCACTCAC AGTCAGGAGA TCAATGAAGT CCTGGATCGC
    CTCGCACATT TAAGCGGTCG GGAGCTTTCT ATCAGGCCGA GTGGCAGCAG GCATATTAAA
    AAACAGACAT TTGTTGTGCA TGCTGGGACA GATACAACTG GTGACATATT CTTCATGGAG
    GTGTGTGATG ATTGCATCGT CTTGCGCAGT AATATTGGCA CAGTCTATGA GCGCTGGTGG
    TATGAGAAGC TCATAAATAT GACTTACTGT CCCAAAACTA AAGTGTTGTG TCTGTGGAGG
    AGAAACGGCC AGGAGACGCA GCTAAACAAG TTTTACACTA AGAAGTGTCG GGAATTGTAC
    TACTGTGTTA AAGACAGCAT GGAGCGTGCT GCTGCTCGAC AACAAAGCAT CAAACCAGGT
    CCAGAGCTTG GAGGCGAGTT TCCGGTTCAG GACATGAAGA CAGGGGAGGG TGGTCTCCTC
    CAAGTCACAC TGGAAGGCAT CAACCTCAAA TTTATGCACA GCCAGGAGCG GAAGGTTTTC
    ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTGAAAG GAGTCTTTGT CCTGGAGGAG
    TTTGTTCCTG AAACTAAAGA AGTGGTGATC CATAAGTACA AGACTCCTAT GGCACATCAG
    ATCTGTTACT CGGTGCTCTG CCTCTTCTCC TACATGGCGG CGGTCAAGGG GAAAGAGTCA
    GAGGGCAAAC CCAAGATGCT GTCCCCTCGT CCACTAGCGA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333562.1
    CDS432..5516
    Translation

    Target ORF information:

    RefSeq Version XM_021477887.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021477887.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA TTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGAACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCAGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT ACCCAAGCTC TGCCCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTTCCCAGC CTCAAAGTAT TTGGGCTAAA TTCTCTAATG
    GAGATTGTAA CAGAGATCGG CCCGGGGAGC GGAGAAGGTG CCCGAGCCCC CAGAGCACTG
    GTAGATCAGA AATCCTCTGT TATTAAGCAC AGCCCTACTG TGAAGAGAGA GTCTCCGTCT
    CCTCAAGGCA GAGCCAACAA TACCAGTGAG AACCAGCAGT TTCTAAAGGA GGTGGTCCAG
    AGCGTGCTGG AGGGGCAGGG TGTGGGCTGG CTTAACATGA AGAAGGTGCG TCGCCTGCTA
    GAGAATGAAC AGCTCAGAGT CTTTGTGCTC AGCAAACTCA ACCGTGCGGT CCAGTCAGAG
    GAGGACGCCC AGCAGGAAGT TATAAGAGAT GTGGAGATAA ATAGAAAGGT CTACAAAGGC
    ATGCTGGATA TTTTGAAGTG CACGGTGTCC AGTTTGGAGC ATTCATACAC TAATGCTGGC
    TTAGGGGGCA TGGCCAGTGT CTTCAGTCTG CTAGAGATAG CACGCACCCA CTACCAAACC
    AAAGATCCAG AGAAGCGGAA ACGGAGCCCC ACCGAAGGGC TTAGCAGTCC AGGCAGTAAG
    GAGAGCCCAT CGGGCCGAAT GGAGAGTGCC AGGGCGGCAG GGGTGCTTCT GGTACCCCGT
    ATCCAATTGC CGCCACCCTC CTCTGGGAAA TCATCACGCC AGTTTGATAC CCGGAGTCTG
    AACGAGGAGA ATTTTATTGC CTCCATTGAA TTGTGGAGCA AGCACCAGGA TAACAGAAAG
    CAAAAAGCTT TGGAAAAAGA ACAGAGTGGT TTAAATAAAC GGAAATCAGG AGCGGATGGA
    GTAAAACAAC GTGTAGAAGG AGGAGACACT GAGGAGAAGA AATCACAGAT CAGTGCAGAC
    AGTGGCCTCA GTGTGACATC TGGCTCACAG AAGAGTGATA CGGATTCCCT GGCGAGCTCT
    GAACCTCCTG CTCTGACCAG AAGCACCAGC CAGGACTCAG AGGCCAGCAC AGTGGTAAGT
    AACAGCTCAG GTGAGACGCT CGGTGCAGAC AGTGACCTGA GCAGCACTGC TGGAGATGGC
    CTGACAGGAA GACATGGTCA GCATCTCAAC CTGTCCCGTG GCACACTGTC TGACAGTGAG
    ATTGAGACCA ACCCTGCAAC CAGCTCAGTG TTTGGCAAGA CACACAAGCT GAAGCCAGGA
    GTGAAGGAGC CTGTTGGTGT TAATAAAGCA GCACCTGCTC CACCCGTTGA GGATGTTAGC
    ATGAGGATCT ATCTGTGTGA GGGGCTTCTG GGCCGGGATA AAAGCTCAGT TTGGGACCAG
    TTGGAGGATG CTGCAATGGA AACCTTTTCT CTGAGTAAAG AGCGCTCAAC TCTGTGGGAT
    CAGATGCAGT TCTGGGAGGA CGCCTTCCTG GATGCCGTAA TGCTGGAACG GGAAGGAATG
    GGAATGGATC AGGGACCACA AGAAATGATT GACAGGTATC TTTCTCTGGG AGATCATGAC
    CGCAAGAGAC TGGAGGATGA TGAGGACAGA CTGCTGGCCA CGCTTCTGCA CAATATGATT
    GCTTACATGC TTATGATGAA GGTCGGTAAA AACGACATCA GGAAGAAAGT ACGTCGTCTT
    ATGGGCAAAT CCCACATTGG CCTCACTCAC AGTCAGGAGA TCAATGAAGT CCTGGATCGC
    CTCGCACATT TAAGCGGTCG GGAGCTTTCT ATCAGGCCGA GTGGCAGCAG GCATATTAAA
    AAACAGACAT TTGTTGTGCA TGCTGGGACA GATACAACTG GTGACATATT CTTCATGGAG
    GTGTGTGATG ATTGCATCGT CTTGCGCAGT AATATTGGCA CAGTCTATGA GCGCTGGTGG
    TATGAGAAGC TCATAAATAT GACTTACTGT CCCAAAACTA AAGTGTTGTG TCTGTGGAGG
    AGAAACGGCC AGGAGACGCA GCTAAACAAG TTTTACACTA AGAAGTGTCG GGAATTGTAC
    TACTGTGTTA AAGACAGCAT GGAGCGTGCT GCTGCTCGAC AACAAAGCAT CAAACCAGGT
    CCAGAGCTTG GAGGCGAGTT TCCGGTTCAG GACATGAAGA CAGGGGAGGG TGGTCTCCTC
    CAAGTCACAC TGGAAGGCAT CAACCTCAAA TTTATGCACA GCCAGGAGCG GAAGGTTTTC
    ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTGAAAG GAGTCTTTGT CCTGGAGGAG
    TTTGTTCCTG AAACTAAAGA AGTGGTGATC CATAAGTACA AGACTCCTAT GGCACATCAG
    ATCTGTTACT CGGTGCTCTG CCTCTTCTCC TACATGGCGG CGGTCAAGGG GAAAGAGTCA
    GAGGGCAAAC CCAAGATGCT GTCCCCTCGT CCACTAGCGA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa143477
    Clone ID Related Accession (Same CDS sequence) XM_021477888.1
    Accession Version XM_021477888.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5082bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA TTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGAACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCAGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT ACCCAAGCTC TGCCCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTTCCCAGC CTCAAAGAAT ATTTTAATTT TGATTTAGCA
    GAGGACACAG ATCAAGACGA TGCTGATGAC AGTCCTGAAA GTCCTGTATT TGGGCTAAAT
    TCTCTAATGG AGATTGTAAC AGAGATCGGC CCGGGGAGCG GAGAAGGTGC CCGAGCCCCC
    AGAGCACTGG TAGATCAGAA ATCCTCTGTT ATTAAGCACA GCCCTACTGT GAAGAGAGAG
    TCTCCGTCTC CTCAAGGCAG AGCCAACAAT ACCAGTGAGA ACCAGCAGTT TCTAAAGGAG
    GTGGTCCAGA GCGTGCTGGA GGGGCAGGGT GTGGGCTGGC TTAACATGAA GAAGGTGCGT
    CGCCTGCTAG AGAATGAACA GCTCAGAGTC TTTGTGCTCA GCAAACTCAA CCGTGCGGTC
    CAGTCAGAGG AGGACGCCCA GCAGGAAGTT ATAAGAGATG TGGAGATAAA TAGAAAGGTC
    TACAAAGGCA TGCTGGATAT TTTGAAGTGC ACGGTGTCCA GTTTGGAGCA TTCATACACT
    AATGCTGGCT TAGGGGGCAT GGCCAGTGTC TTCAGTCTGC TAGAGATAGC ACGCACCCAC
    TACCAAACCA AAGATCCAGA GAAGCGGAAA CGGAGCCCCA CCGAAGGGCT TAGCAGTCCA
    GGCAGTAAGG AGAGCCCATC GGGCCGAATG GAGAGTGCCA GGGCGGCAGG GGTGCTTCTG
    GTACCCCGTA TCCAATTGCC GCCACCCTCC TCTGGGAAAT CATCACGCCA GTTTGATACC
    CGGAGTCTGA ACGAGGAGAA TTTTATTGCC TCCATTGAAT TGTGGAGCAA GCACCAGGAT
    AACAGAAAGC AAAAAGCTTT GGAAAAAGAA CAGAGTGGTT TAAATAAACG GAAATCAGGA
    GCGGATGGAG TAAAACAACG TGTAGAAGGA GGAGACACTG AGGAGAAGAA ATCACAGATC
    AGTGCAGACA GTGGCCTCAG TGTGACATCT GGCTCACAGA AGAGTGATAC GGATTCCCTG
    GCGAGCTCTG AACCTCCTGC TCTGACCAGA AGCACCAGCC AGGACTCAGA GGCCAGCACA
    GTGGTAAGTA ACAGCTCAGG TGAGACGCTC GGTGCAGACA GTGACCTGAG CAGCACTGCT
    GGAGATGGCC TGACAGGAAG ACATGGTCAG CATCTCAACC TGTCCCGTGG CACACTGTCT
    GACAGTGAGA TTGAGACCAA CCCTGCAACC AGCTCAGTGT TTGGCAAGAC ACACAAGCTG
    AAGCCAGGAG TGAAGGAGCC TGTTGGTGTT AATAAAGCAG CACCTGCTCC ACCCGTTGAG
    GATGTTAGCA TGAGGATCTA TCTGTGTGAG GGGCTTCTGG GTAAAGAGCG CTCAACTCTG
    TGGGATCAGA TGCAGTTCTG GGAGGACGCC TTCCTGGATG CCGTAATGCT GGAACGGGAA
    GGAATGGGAA TGGATCAGGG ACCACAAGAA ATGATTGACA GGTATCTTTC TCTGGGAGAT
    CATGACCGCA AGAGACTGGA GGATGATGAG GACAGACTGC TGGCCACGCT TCTGCACAAT
    ATGATTGCTT ACATGCTTAT GATGAAGGTC GGTAAAAACG ACATCAGGAA GAAAGTACGT
    CGTCTTATGG GCAAATCCCA CATTGGCCTC ACTCACAGTC AGGAGATCAA TGAAGTCCTG
    GATCGCCTCG CACATTTAAG CGGTCGGGAG CTTTCTATCA GGCCGAGTGG CAGCAGGCAT
    ATTAAAAAAC AGACATTTGT TGTGCATGCT GGGACAGATA CAACTGGTGA CATATTCTTC
    ATGGAGGTGT GTGATGATTG CATCGTCTTG CGCAGTAATA TTGGCACAGT CTATGAGCGC
    TGGTGGTATG AGAAGCTCAT AAATATGACT TACTGTCCCA AAACTAAAGT GTTGTGTCTG
    TGGAGGAGAA ACGGCCAGGA GACGCAGCTA AACAAGTTTT ACACTAAGAA GTGTCGGGAA
    TTGTACTACT GTGTTAAAGA CAGCATGGAG CGTGCTGCTG CTCGACAACA AAGCATCAAA
    CCAGGTCCAG AGCTTGGAGG CGAGTTTCCG GTTCAGGACA TGAAGACAGG GGAGGGTGGT
    CTCCTCCAAG TCACACTGGA AGGCATCAAC CTCAAATTTA TGCACAGCCA GGTTTTCATA
    GAGCTGAATC ACATTAAAAA GTGCAATACA GTGAAAGGAG TCTTTGTCCT GGAGGAGTTT
    GTTCCTGAAA CTAAAGAAGT GGTGATCCAT AAGTACAAGA CTCCTATGGC ACATCAGATC
    TGTTACTCGG TGCTCTGCCT CTTCTCCTAC ATGGCGGCGG TCAAGGGGAA AGAGTCAGAG
    GGCAAACCCA AGATGCTGTC CCCTCGTCCA CTAGCGAGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333563.1
    CDS432..5513
    Translation

    Target ORF information:

    RefSeq Version XM_021477888.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021477888.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA TTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGAACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCAGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT ACCCAAGCTC TGCCCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTTCCCAGC CTCAAAGAAT ATTTTAATTT TGATTTAGCA
    GAGGACACAG ATCAAGACGA TGCTGATGAC AGTCCTGAAA GTCCTGTATT TGGGCTAAAT
    TCTCTAATGG AGATTGTAAC AGAGATCGGC CCGGGGAGCG GAGAAGGTGC CCGAGCCCCC
    AGAGCACTGG TAGATCAGAA ATCCTCTGTT ATTAAGCACA GCCCTACTGT GAAGAGAGAG
    TCTCCGTCTC CTCAAGGCAG AGCCAACAAT ACCAGTGAGA ACCAGCAGTT TCTAAAGGAG
    GTGGTCCAGA GCGTGCTGGA GGGGCAGGGT GTGGGCTGGC TTAACATGAA GAAGGTGCGT
    CGCCTGCTAG AGAATGAACA GCTCAGAGTC TTTGTGCTCA GCAAACTCAA CCGTGCGGTC
    CAGTCAGAGG AGGACGCCCA GCAGGAAGTT ATAAGAGATG TGGAGATAAA TAGAAAGGTC
    TACAAAGGCA TGCTGGATAT TTTGAAGTGC ACGGTGTCCA GTTTGGAGCA TTCATACACT
    AATGCTGGCT TAGGGGGCAT GGCCAGTGTC TTCAGTCTGC TAGAGATAGC ACGCACCCAC
    TACCAAACCA AAGATCCAGA GAAGCGGAAA CGGAGCCCCA CCGAAGGGCT TAGCAGTCCA
    GGCAGTAAGG AGAGCCCATC GGGCCGAATG GAGAGTGCCA GGGCGGCAGG GGTGCTTCTG
    GTACCCCGTA TCCAATTGCC GCCACCCTCC TCTGGGAAAT CATCACGCCA GTTTGATACC
    CGGAGTCTGA ACGAGGAGAA TTTTATTGCC TCCATTGAAT TGTGGAGCAA GCACCAGGAT
    AACAGAAAGC AAAAAGCTTT GGAAAAAGAA CAGAGTGGTT TAAATAAACG GAAATCAGGA
    GCGGATGGAG TAAAACAACG TGTAGAAGGA GGAGACACTG AGGAGAAGAA ATCACAGATC
    AGTGCAGACA GTGGCCTCAG TGTGACATCT GGCTCACAGA AGAGTGATAC GGATTCCCTG
    GCGAGCTCTG AACCTCCTGC TCTGACCAGA AGCACCAGCC AGGACTCAGA GGCCAGCACA
    GTGGTAAGTA ACAGCTCAGG TGAGACGCTC GGTGCAGACA GTGACCTGAG CAGCACTGCT
    GGAGATGGCC TGACAGGAAG ACATGGTCAG CATCTCAACC TGTCCCGTGG CACACTGTCT
    GACAGTGAGA TTGAGACCAA CCCTGCAACC AGCTCAGTGT TTGGCAAGAC ACACAAGCTG
    AAGCCAGGAG TGAAGGAGCC TGTTGGTGTT AATAAAGCAG CACCTGCTCC ACCCGTTGAG
    GATGTTAGCA TGAGGATCTA TCTGTGTGAG GGGCTTCTGG GTAAAGAGCG CTCAACTCTG
    TGGGATCAGA TGCAGTTCTG GGAGGACGCC TTCCTGGATG CCGTAATGCT GGAACGGGAA
    GGAATGGGAA TGGATCAGGG ACCACAAGAA ATGATTGACA GGTATCTTTC TCTGGGAGAT
    CATGACCGCA AGAGACTGGA GGATGATGAG GACAGACTGC TGGCCACGCT TCTGCACAAT
    ATGATTGCTT ACATGCTTAT GATGAAGGTC GGTAAAAACG ACATCAGGAA GAAAGTACGT
    CGTCTTATGG GCAAATCCCA CATTGGCCTC ACTCACAGTC AGGAGATCAA TGAAGTCCTG
    GATCGCCTCG CACATTTAAG CGGTCGGGAG CTTTCTATCA GGCCGAGTGG CAGCAGGCAT
    ATTAAAAAAC AGACATTTGT TGTGCATGCT GGGACAGATA CAACTGGTGA CATATTCTTC
    ATGGAGGTGT GTGATGATTG CATCGTCTTG CGCAGTAATA TTGGCACAGT CTATGAGCGC
    TGGTGGTATG AGAAGCTCAT AAATATGACT TACTGTCCCA AAACTAAAGT GTTGTGTCTG
    TGGAGGAGAA ACGGCCAGGA GACGCAGCTA AACAAGTTTT ACACTAAGAA GTGTCGGGAA
    TTGTACTACT GTGTTAAAGA CAGCATGGAG CGTGCTGCTG CTCGACAACA AAGCATCAAA
    CCAGGTCCAG AGCTTGGAGG CGAGTTTCCG GTTCAGGACA TGAAGACAGG GGAGGGTGGT
    CTCCTCCAAG TCACACTGGA AGGCATCAAC CTCAAATTTA TGCACAGCCA GGTTTTCATA
    GAGCTGAATC ACATTAAAAA GTGCAATACA GTGAAAGGAG TCTTTGTCCT GGAGGAGTTT
    GTTCCTGAAA CTAAAGAAGT GGTGATCCAT AAGTACAAGA CTCCTATGGC ACATCAGATC
    TGTTACTCGG TGCTCTGCCT CTTCTCCTAC ATGGCGGCGG TCAAGGGGAA AGAGTCAGAG
    GGCAAACCCA AGATGCTGTC CCCTCGTCCA CTAGCGAGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa143478
    Clone ID Related Accession (Same CDS sequence) XM_021477889.1
    Accession Version XM_021477889.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5079bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGGG AACATTCGGC GCCCCCTGCA
    GGAGATGGGA CTTTGCTGCC AGGCGAGCCG GGTAGCAGCG GGAAGTCTCC GCGATCTAAA
    CGCAGTGGCC GAATAGCACC ACAGAACCGC AACAGCATGT TAACATCTCT GTGTATTCTG
    AGCCACTACC CTTTCTTCTC AACATTCAGA GAGTGTCTGT ACATCCTCAA GCGCATGGTG
    GACTGCTGTA GTCAGCGGCT CAACCAGAGA CCCGGAGCTC CCAAATCCAC ACAACGGGAC
    ACGATGTGGC GTGTGTTCAC GGGAGCTCTT TCTGTGGAAG AAAAGGAGAA GGGCAGTCAG
    GTGCTCCAGG ACCTGAGGGA GATCGAGTCA TGGGTTTATC GGCTGCTGCG CTCACCGGTG
    CCTGTTGCAG GTCAGCGGCG TGTGGATGTG GAGGTCCTGC CCCATGAGCT GCAGCCTGCT
    CTGACATTTG CCCTTCCAGA CCCGTCCCGC TTCAGCATCG TAGACTTCCC GCTCCACCTG
    CCACTGGAGC TGCTGGGAGT CGATGCCTGT CTGCAGGTCT TGGCCTGCAT TCTGCTGGAG
    CACAAGGTGG TACTGCAGTC TCGAGATTAT AACGCTCTCT CAATGAGCGT CATGGCGTTT
    GTTTCGATGA TTTACCCTCT TGAGTACATG TTTCCTGTCA TTCCACTGTT GCCCACCTGC
    ATGGCCTCTG CAGAACAGCT CCTATTAGCA CCCACTCCAT ACGTCATTGG TGTGCCAGCC
    AGTTTCTTCT TGTACAAGTG TGATTTTAAG ATGCCTGATG ATGTTTGGTT AGTGGATCTG
    GACTGCAACA AGGTTATTGT TCCAAGCAAT GCAGAACTGC TCCCACCCTT ACCTGAACCT
    GAGGCCTCTG AGCTGAAGAA ACACTTGAAG CAGCTCTTGG AGTGTCTGGT AAGGTTGACT
    CTGATCACCC AAAAGCAGAT CTTCGCCTCC GATTCTAAGG CTCTGGCTAG CATGAGTCTA
    AACACACAGC CTATTCTGAA CCTGGAGAAG TTTCAGGATG GACAGGAGTT GTCTTTATTG
    CCCCCCGGTC GTGACAAAGC TTCCCCGTCC TCCACTGAGT TCAACCCACT CATCTACGGC
    AATGATGTGG ACTCAGTTGA TGTTGCCACA AGGGTGGCAA TGGTGAGGTT CTTCAACTCT
    CCAAACGTGC TGCAAGGGTT CCAGATGCAC ACACGCACTC TCCGTCTCTT CCCTCGTCCT
    GTGGTGGCCT TTCAGTCCAC ATCTTTCCTG GCTTCTAGGC CACGACGAAA TGGATTCACA
    GAAAAGCTCT CCCACACTCA GGCAGTGGAG TACTATGGAG AATGGGCTCT CAACCCCACC
    AACCTGGCTT TCCAAAGGAT TCATAACAAT GTGTATGACC CCTCTCTAAT AGGCGATAAG
    CCTAAGTGGT ATGCCCACCA GTTACAGCCA GTATTCTACC GCGTGTATGA TGGAAACTCT
    CATTTAGCGG AGGCCTTGAG TGGACCCCTG CAGGATGAGA CCAATGATTC AGACCCAACA
    GACGACAGTG GCAGCGACAG TGAAGCATAT GATGACTCCA GCTCCTCCTA CTCATCTCTT
    GGAGATTTTG TGAATGAGAT GATCAAAGGG GACATACAGG GAGACACACC AAATGTTGAT
    CCCCTGACCC ATGCTGCACT GGGAGATGCA GATGAGGTTG AGATCCATGA CTTTCACGAG
    TATAAGGGGA ACAGCGGTGA ACCAGAGCCA GAAGGCCCCA CAGAAGCAGC GGACAGTCAG
    CCCTTGCGGT CCAGCTCTAG CACCACAGCC AGTTCCAGCC CCAGTACAGT CATACAGGGA
    GTCAACCATG AGCAGAAGGA ACCTGTTGAG GTGGAGCCCA TAGCAAATGT GACTTACCCA
    AGCTCTGCCC CAGTATTGGG TCCTCCTCCT TTTACAAGAC CCACCCCTGA TCCAACCCTT
    GTTGACCCTG CCAATAAGAA GCGAGAATAT GACAACCCCT ATTTTGAGCC CCAGTACGGT
    TTCCCTACAG AGGAAGATGC TGAGACTGAT GAGCAGGAGG AGAGTTACAC ACCACGATTT
    AACCAGAATC TCAATGGCAA TAAGCCTTCT CGTCCACTGA GACCCAGCAG CCTGAAGCTT
    CCGGGTGAAT CTGACGGAGA GGGAGACTCC AGAAATAGCT CTCCTAACTC AACCATCTCC
    AACAATAGCA GTGATGGCTT TGGGGGACTC ATGTCCTTTG CCAGTAACTT GTATAAAAAC
    CATGGCACCA GTTTCAGTCT GTCCAGCCTG GCTCTGCCTA ATAAAGCGAG AGAGAAGAAC
    ACGCCGTTTC CCAGCCTCAA AGAATATTTT AATTTTGATT TAGCAGAGGA CACAGATCAA
    GACGATGCTG ATGACAGTCC TGAAAGTCCT GTATTTGGGC TAAATTCTCT AATGGAGATT
    GTAACAGAGA TCGGCCCGGG GAGCGGAGAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGAATTGTGG AGCAAGCACC AGGATAACAG AAAGCAAAAA
    GCTTTGGAAA AAGAACAGAG TGGTTTAAAT AAACGGAAAT CAGGAGCGGA TGGAGTAAAA
    CAACGTGTAG AAGGAGGAGA CACTGAGGAG AAGAAATCAC AGATCAGTGC AGACAGTGGC
    CTCAGTGTGA CATCTGGCTC ACAGAAGAGT GATACGGATT CCCTGGCGAG CTCTGAACCT
    CCTGCTCTGA CCAGAAGCAC CAGCCAGGAC TCAGAGGCCA GCACAGTGGT AAGTAACAGC
    TCAGGTGAGA CGCTCGGTGC AGACAGTGAC CTGAGCAGCA CTGCTGGAGA TGGCCTGACA
    GGAAGACATG GTCAGCATCT CAACCTGTCC CGTGGCACAC TGTCTGACAG TGAGATTGAG
    ACCAACCCTG CAACCAGCTC AGTGTTTGGC AAGACACACA AGCTGAAGCC AGGAGTGAAG
    GAGCCTGTTG GTGTTAATAA AGCAGCACCT GCTCCACCCG TTGAGGATGT TAGCATGAGG
    ATCTATCTGT GTGAGGGGCT TCTGGGCCGG GATAAAAGCT CAGTTTGGGA CCAGTTGGAG
    GATGCTGCAA TGGAAACCTT TTCTCTGAGT AAAGAGCGCT CAACTCTGTG GGATCAGATG
    CAGTTCTGGG AGGACGCCTT CCTGGATGCC GTAATGCTGG AACGGGAAGG AATGGGAATG
    GATCAGGGAC CACAAGAAAT GATTGACAGG TATCTTTCTC TGGGAGATCA TGACCGCAAG
    AGACTGGAGG ATGATGAGGA CAGACTGCTG GCCACGCTTC TGCACAATAT GATTGCTTAC
    ATGCTTATGA TGAAGGTCGG TAAAAACGAC ATCAGGAAGA AAGTACGTCG TCTTATGGGC
    AAATCCCACA TTGGCCTCAC TCACAGTCAG GAGATCAATG AAGTCCTGGA TCGCCTCGCA
    CATTTAAGCG GTCGGGAGCT TTCTATCAGG CCGAGTGGCA GCAGGCATAT TAAAAAACAG
    ACATTTGTTG TGCATGCTGG GACAGATACA ACTGGTGACA TATTCTTCAT GGAGGTGTGT
    GATGATTGCA TCGTCTTGCG CAGTAATATT GGCACAGTCT ATGAGCGCTG GTGGTATGAG
    AAGCTCATAA ATATGACTTA CTGTCCCAAA ACTAAAGTGT TGTGTCTGTG GAGGAGAAAC
    GGCCAGGAGA CGCAGCTAAA CAAGTTTTAC ACTAAGAAGT GTCGGGAATT GTACTACTGT
    GTTAAAGACA GCATGGAGCG TGCTGCTGCT CGACAACAAA GCATCAAACC AGGTCCAGAG
    CTTGGAGGCG AGTTTCCGGT TCAGGACATG AAGACAGGGG AGGGTGGTCT CCTCCAAGTC
    ACACTGGAAG GCATCAACCT CAAATTTATG CACAGCCAGG AGCGGAAGGT TTTCATAGAG
    CTGAATCACA TTAAAAAGTG CAATACAGTG AAAGGAGTCT TTGTCCTGGA GGAGTTTGTT
    CCTGAAACTA AAGAAGTGGT GATCCATAAG TACAAGACTC CTATGGCACA TCAGATCTGT
    TACTCGGTGC TCTGCCTCTT CTCCTACATG GCGGCGGTCA AGGGGAAAGA GTCAGAGGGC
    AAACCCAAGA TGCTGTCCCC TCGTCCACTA GCGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333564.1
    CDS432..5510
    Translation

    Target ORF information:

    RefSeq Version XM_021477889.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021477889.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGGG AACATTCGGC GCCCCCTGCA
    GGAGATGGGA CTTTGCTGCC AGGCGAGCCG GGTAGCAGCG GGAAGTCTCC GCGATCTAAA
    CGCAGTGGCC GAATAGCACC ACAGAACCGC AACAGCATGT TAACATCTCT GTGTATTCTG
    AGCCACTACC CTTTCTTCTC AACATTCAGA GAGTGTCTGT ACATCCTCAA GCGCATGGTG
    GACTGCTGTA GTCAGCGGCT CAACCAGAGA CCCGGAGCTC CCAAATCCAC ACAACGGGAC
    ACGATGTGGC GTGTGTTCAC GGGAGCTCTT TCTGTGGAAG AAAAGGAGAA GGGCAGTCAG
    GTGCTCCAGG ACCTGAGGGA GATCGAGTCA TGGGTTTATC GGCTGCTGCG CTCACCGGTG
    CCTGTTGCAG GTCAGCGGCG TGTGGATGTG GAGGTCCTGC CCCATGAGCT GCAGCCTGCT
    CTGACATTTG CCCTTCCAGA CCCGTCCCGC TTCAGCATCG TAGACTTCCC GCTCCACCTG
    CCACTGGAGC TGCTGGGAGT CGATGCCTGT CTGCAGGTCT TGGCCTGCAT TCTGCTGGAG
    CACAAGGTGG TACTGCAGTC TCGAGATTAT AACGCTCTCT CAATGAGCGT CATGGCGTTT
    GTTTCGATGA TTTACCCTCT TGAGTACATG TTTCCTGTCA TTCCACTGTT GCCCACCTGC
    ATGGCCTCTG CAGAACAGCT CCTATTAGCA CCCACTCCAT ACGTCATTGG TGTGCCAGCC
    AGTTTCTTCT TGTACAAGTG TGATTTTAAG ATGCCTGATG ATGTTTGGTT AGTGGATCTG
    GACTGCAACA AGGTTATTGT TCCAAGCAAT GCAGAACTGC TCCCACCCTT ACCTGAACCT
    GAGGCCTCTG AGCTGAAGAA ACACTTGAAG CAGCTCTTGG AGTGTCTGGT AAGGTTGACT
    CTGATCACCC AAAAGCAGAT CTTCGCCTCC GATTCTAAGG CTCTGGCTAG CATGAGTCTA
    AACACACAGC CTATTCTGAA CCTGGAGAAG TTTCAGGATG GACAGGAGTT GTCTTTATTG
    CCCCCCGGTC GTGACAAAGC TTCCCCGTCC TCCACTGAGT TCAACCCACT CATCTACGGC
    AATGATGTGG ACTCAGTTGA TGTTGCCACA AGGGTGGCAA TGGTGAGGTT CTTCAACTCT
    CCAAACGTGC TGCAAGGGTT CCAGATGCAC ACACGCACTC TCCGTCTCTT CCCTCGTCCT
    GTGGTGGCCT TTCAGTCCAC ATCTTTCCTG GCTTCTAGGC CACGACGAAA TGGATTCACA
    GAAAAGCTCT CCCACACTCA GGCAGTGGAG TACTATGGAG AATGGGCTCT CAACCCCACC
    AACCTGGCTT TCCAAAGGAT TCATAACAAT GTGTATGACC CCTCTCTAAT AGGCGATAAG
    CCTAAGTGGT ATGCCCACCA GTTACAGCCA GTATTCTACC GCGTGTATGA TGGAAACTCT
    CATTTAGCGG AGGCCTTGAG TGGACCCCTG CAGGATGAGA CCAATGATTC AGACCCAACA
    GACGACAGTG GCAGCGACAG TGAAGCATAT GATGACTCCA GCTCCTCCTA CTCATCTCTT
    GGAGATTTTG TGAATGAGAT GATCAAAGGG GACATACAGG GAGACACACC AAATGTTGAT
    CCCCTGACCC ATGCTGCACT GGGAGATGCA GATGAGGTTG AGATCCATGA CTTTCACGAG
    TATAAGGGGA ACAGCGGTGA ACCAGAGCCA GAAGGCCCCA CAGAAGCAGC GGACAGTCAG
    CCCTTGCGGT CCAGCTCTAG CACCACAGCC AGTTCCAGCC CCAGTACAGT CATACAGGGA
    GTCAACCATG AGCAGAAGGA ACCTGTTGAG GTGGAGCCCA TAGCAAATGT GACTTACCCA
    AGCTCTGCCC CAGTATTGGG TCCTCCTCCT TTTACAAGAC CCACCCCTGA TCCAACCCTT
    GTTGACCCTG CCAATAAGAA GCGAGAATAT GACAACCCCT ATTTTGAGCC CCAGTACGGT
    TTCCCTACAG AGGAAGATGC TGAGACTGAT GAGCAGGAGG AGAGTTACAC ACCACGATTT
    AACCAGAATC TCAATGGCAA TAAGCCTTCT CGTCCACTGA GACCCAGCAG CCTGAAGCTT
    CCGGGTGAAT CTGACGGAGA GGGAGACTCC AGAAATAGCT CTCCTAACTC AACCATCTCC
    AACAATAGCA GTGATGGCTT TGGGGGACTC ATGTCCTTTG CCAGTAACTT GTATAAAAAC
    CATGGCACCA GTTTCAGTCT GTCCAGCCTG GCTCTGCCTA ATAAAGCGAG AGAGAAGAAC
    ACGCCGTTTC CCAGCCTCAA AGAATATTTT AATTTTGATT TAGCAGAGGA CACAGATCAA
    GACGATGCTG ATGACAGTCC TGAAAGTCCT GTATTTGGGC TAAATTCTCT AATGGAGATT
    GTAACAGAGA TCGGCCCGGG GAGCGGAGAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGAATTGTGG AGCAAGCACC AGGATAACAG AAAGCAAAAA
    GCTTTGGAAA AAGAACAGAG TGGTTTAAAT AAACGGAAAT CAGGAGCGGA TGGAGTAAAA
    CAACGTGTAG AAGGAGGAGA CACTGAGGAG AAGAAATCAC AGATCAGTGC AGACAGTGGC
    CTCAGTGTGA CATCTGGCTC ACAGAAGAGT GATACGGATT CCCTGGCGAG CTCTGAACCT
    CCTGCTCTGA CCAGAAGCAC CAGCCAGGAC TCAGAGGCCA GCACAGTGGT AAGTAACAGC
    TCAGGTGAGA CGCTCGGTGC AGACAGTGAC CTGAGCAGCA CTGCTGGAGA TGGCCTGACA
    GGAAGACATG GTCAGCATCT CAACCTGTCC CGTGGCACAC TGTCTGACAG TGAGATTGAG
    ACCAACCCTG CAACCAGCTC AGTGTTTGGC AAGACACACA AGCTGAAGCC AGGAGTGAAG
    GAGCCTGTTG GTGTTAATAA AGCAGCACCT GCTCCACCCG TTGAGGATGT TAGCATGAGG
    ATCTATCTGT GTGAGGGGCT TCTGGGCCGG GATAAAAGCT CAGTTTGGGA CCAGTTGGAG
    GATGCTGCAA TGGAAACCTT TTCTCTGAGT AAAGAGCGCT CAACTCTGTG GGATCAGATG
    CAGTTCTGGG AGGACGCCTT CCTGGATGCC GTAATGCTGG AACGGGAAGG AATGGGAATG
    GATCAGGGAC CACAAGAAAT GATTGACAGG TATCTTTCTC TGGGAGATCA TGACCGCAAG
    AGACTGGAGG ATGATGAGGA CAGACTGCTG GCCACGCTTC TGCACAATAT GATTGCTTAC
    ATGCTTATGA TGAAGGTCGG TAAAAACGAC ATCAGGAAGA AAGTACGTCG TCTTATGGGC
    AAATCCCACA TTGGCCTCAC TCACAGTCAG GAGATCAATG AAGTCCTGGA TCGCCTCGCA
    CATTTAAGCG GTCGGGAGCT TTCTATCAGG CCGAGTGGCA GCAGGCATAT TAAAAAACAG
    ACATTTGTTG TGCATGCTGG GACAGATACA ACTGGTGACA TATTCTTCAT GGAGGTGTGT
    GATGATTGCA TCGTCTTGCG CAGTAATATT GGCACAGTCT ATGAGCGCTG GTGGTATGAG
    AAGCTCATAA ATATGACTTA CTGTCCCAAA ACTAAAGTGT TGTGTCTGTG GAGGAGAAAC
    GGCCAGGAGA CGCAGCTAAA CAAGTTTTAC ACTAAGAAGT GTCGGGAATT GTACTACTGT
    GTTAAAGACA GCATGGAGCG TGCTGCTGCT CGACAACAAA GCATCAAACC AGGTCCAGAG
    CTTGGAGGCG AGTTTCCGGT TCAGGACATG AAGACAGGGG AGGGTGGTCT CCTCCAAGTC
    ACACTGGAAG GCATCAACCT CAAATTTATG CACAGCCAGG AGCGGAAGGT TTTCATAGAG
    CTGAATCACA TTAAAAAGTG CAATACAGTG AAAGGAGTCT TTGTCCTGGA GGAGTTTGTT
    CCTGAAACTA AAGAAGTGGT GATCCATAAG TACAAGACTC CTATGGCACA TCAGATCTGT
    TACTCGGTGC TCTGCCTCTT CTCCTACATG GCGGCGGTCA AGGGGAAAGA GTCAGAGGGC
    AAACCCAAGA TGCTGTCCCC TCGTCCACTA GCGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa143479
    Clone ID Related Accession (Same CDS sequence) XM_021477890.1
    Accession Version XM_021477890.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5073bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA TTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGAACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCAGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT ACCCAAGCTC TGCCCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTTCCCAGC CTCAAAGAAT ATTTTAATTT TGATTTAGCA
    GAGGACACAG ATCAAGACGA TGCTGATGAC AGTCCTGAAA GTCCTGTATT TGGGCTAAAT
    TCTCTAATGG AGATTGTAAC AGAGATCGGC CCGGGGAGCG GAGAAGGTGC CCGAGCCCCC
    AGAGCACTGG TAGATCAGAA ATCCTCTGTT ATTAAGCACA GCCCTACTGT GAAGAGAGAG
    TCTCCGTCTC CTCAAGGCAG AGCCAACAAT ACCAGTGAGA ACCAGCAGTT TCTAAAGGAG
    GTGGTCCAGA GCGTGCTGGA GGGGCAGGGT GTGGGCTGGC TTAACATGAA GAAGGTGCGT
    CGCCTGCTAG AGAATGAACA GCTCAGAGTC TTTGTGCTCA GCAAACTCAA CCGTGCGGTC
    CAGTCAGAGG AGGACGCCCA GCAGGAAGTT ATAAGAGATG TGGAGATAAA TAGAAAGGTC
    TACAAAGGCA TGCTGGATAT TTTGAAGTGC ACGGTGTCCA GTTTGGAGCA TTCATACACT
    AATGCTGGCT TAGGGGGCAT GGCCAGTGTC TTCAGTCTGC TAGAGATAGC ACGCACCCAC
    TACCAAACCA AAGATCCAGA GAAGCGGAAA CGGAGCCCCA CCGAAGGGCT TAGCAGTCCA
    GGCAGTAAGG AGAGCCCATC GGGCCGAATG GAGAGTGCCA GGGCGGCAGG GGTGCTTCTG
    GTACCCCGTA TCCAATTGCC GCCACCCTCC TCTGGGAAAT CATCACGCCA GTTTGATACC
    CGGAGTCTGA ACGAGGAGAA TTTTATTGCC TCCATTGGAG CGGATGGAGT AAAACAACGT
    GTAGAAGGAG GAGACACTGA GGAGAAGAAA TCACAGATCA GTGCAGACAG TGGCCTCAGT
    GTGACATCTG GCTCACAGAA GAGTGATACG GATTCCCTGG CGAGCTCTGA ACCTCCTGCT
    CTGACCAGAA GCACCAGCCA GGACTCAGAG GCCAGCACAG TGGTAAGTAA CAGCTCAGGT
    GAGACGCTCG GTGCAGACAG TGACCTGAGC AGCACTGCTG GAGATGGCCT GACAGGAAGA
    CATGGTCAGC ATCTCAACCT GTCCCGTGGC ACACTGTCTG ACAGTGAGAT TGAGACCAAC
    CCTGCAACCA GCTCAGTGTT TGGCAAGACA CACAAGCTGA AGCCAGGAGT GAAGGAGCCT
    GTTGGTGTTA ATAAAGCAGC ACCTGCTCCA CCCGTTGAGG ATGTTAGCAT GAGGATCTAT
    CTGTGTGAGG GGCTTCTGGG CCGGGATAAA AGCTCAGTTT GGGACCAGTT GGAGGATGCT
    GCAATGGAAA CCTTTTCTCT GAGTAAAGAG CGCTCAACTC TGTGGGATCA GATGCAGTTC
    TGGGAGGACG CCTTCCTGGA TGCCGTAATG CTGGAACGGG AAGGAATGGG AATGGATCAG
    GGACCACAAG AAATGATTGA CAGGTATCTT TCTCTGGGAG ATCATGACCG CAAGAGACTG
    GAGGATGATG AGGACAGACT GCTGGCCACG CTTCTGCACA ATATGATTGC TTACATGCTT
    ATGATGAAGG TCGGTAAAAA CGACATCAGG AAGAAAGTAC GTCGTCTTAT GGGCAAATCC
    CACATTGGCC TCACTCACAG TCAGGAGATC AATGAAGTCC TGGATCGCCT CGCACATTTA
    AGCGGTCGGG AGCTTTCTAT CAGGCCGAGT GGCAGCAGGC ATATTAAAAA ACAGACATTT
    GTTGTGCATG CTGGGACAGA TACAACTGGT GACATATTCT TCATGGAGGT GTGTGATGAT
    TGCATCGTCT TGCGCAGTAA TATTGGCACA GTCTATGAGC GCTGGTGGTA TGAGAAGCTC
    ATAAATATGA CTTACTGTCC CAAAACTAAA GTGTTGTGTC TGTGGAGGAG AAACGGCCAG
    GAGACGCAGC TAAACAAGTT TTACACTAAG AAGTGTCGGG AATTGTACTA CTGTGTTAAA
    GACAGCATGG AGCGTGCTGC TGCTCGACAA CAAAGCATCA AACCAGGTCC AGAGCTTGGA
    GGCGAGTTTC CGGTTCAGGA CATGAAGACA GGGGAGGGTG GTCTCCTCCA AGTCACACTG
    GAAGGCATCA ACCTCAAATT TATGCACAGC CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT
    CACATTAAAA AGTGCAATAC AGTGAAAGGA GTCTTTGTCC TGGAGGAGTT TGTTCCTGAA
    ACTAAAGAAG TGGTGATCCA TAAGTACAAG ACTCCTATGG CACATCAGAT CTGTTACTCG
    GTGCTCTGCC TCTTCTCCTA CATGGCGGCG GTCAAGGGGA AAGAGTCAGA GGGCAAACCC
    AAGATGCTGT CCCCTCGTCC ACTAGCGAGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333565.1
    CDS432..5504
    Translation

    Target ORF information:

    RefSeq Version XM_021477890.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021477890.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA TTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGAACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCAGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT ACCCAAGCTC TGCCCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTTCCCAGC CTCAAAGAAT ATTTTAATTT TGATTTAGCA
    GAGGACACAG ATCAAGACGA TGCTGATGAC AGTCCTGAAA GTCCTGTATT TGGGCTAAAT
    TCTCTAATGG AGATTGTAAC AGAGATCGGC CCGGGGAGCG GAGAAGGTGC CCGAGCCCCC
    AGAGCACTGG TAGATCAGAA ATCCTCTGTT ATTAAGCACA GCCCTACTGT GAAGAGAGAG
    TCTCCGTCTC CTCAAGGCAG AGCCAACAAT ACCAGTGAGA ACCAGCAGTT TCTAAAGGAG
    GTGGTCCAGA GCGTGCTGGA GGGGCAGGGT GTGGGCTGGC TTAACATGAA GAAGGTGCGT
    CGCCTGCTAG AGAATGAACA GCTCAGAGTC TTTGTGCTCA GCAAACTCAA CCGTGCGGTC
    CAGTCAGAGG AGGACGCCCA GCAGGAAGTT ATAAGAGATG TGGAGATAAA TAGAAAGGTC
    TACAAAGGCA TGCTGGATAT TTTGAAGTGC ACGGTGTCCA GTTTGGAGCA TTCATACACT
    AATGCTGGCT TAGGGGGCAT GGCCAGTGTC TTCAGTCTGC TAGAGATAGC ACGCACCCAC
    TACCAAACCA AAGATCCAGA GAAGCGGAAA CGGAGCCCCA CCGAAGGGCT TAGCAGTCCA
    GGCAGTAAGG AGAGCCCATC GGGCCGAATG GAGAGTGCCA GGGCGGCAGG GGTGCTTCTG
    GTACCCCGTA TCCAATTGCC GCCACCCTCC TCTGGGAAAT CATCACGCCA GTTTGATACC
    CGGAGTCTGA ACGAGGAGAA TTTTATTGCC TCCATTGGAG CGGATGGAGT AAAACAACGT
    GTAGAAGGAG GAGACACTGA GGAGAAGAAA TCACAGATCA GTGCAGACAG TGGCCTCAGT
    GTGACATCTG GCTCACAGAA GAGTGATACG GATTCCCTGG CGAGCTCTGA ACCTCCTGCT
    CTGACCAGAA GCACCAGCCA GGACTCAGAG GCCAGCACAG TGGTAAGTAA CAGCTCAGGT
    GAGACGCTCG GTGCAGACAG TGACCTGAGC AGCACTGCTG GAGATGGCCT GACAGGAAGA
    CATGGTCAGC ATCTCAACCT GTCCCGTGGC ACACTGTCTG ACAGTGAGAT TGAGACCAAC
    CCTGCAACCA GCTCAGTGTT TGGCAAGACA CACAAGCTGA AGCCAGGAGT GAAGGAGCCT
    GTTGGTGTTA ATAAAGCAGC ACCTGCTCCA CCCGTTGAGG ATGTTAGCAT GAGGATCTAT
    CTGTGTGAGG GGCTTCTGGG CCGGGATAAA AGCTCAGTTT GGGACCAGTT GGAGGATGCT
    GCAATGGAAA CCTTTTCTCT GAGTAAAGAG CGCTCAACTC TGTGGGATCA GATGCAGTTC
    TGGGAGGACG CCTTCCTGGA TGCCGTAATG CTGGAACGGG AAGGAATGGG AATGGATCAG
    GGACCACAAG AAATGATTGA CAGGTATCTT TCTCTGGGAG ATCATGACCG CAAGAGACTG
    GAGGATGATG AGGACAGACT GCTGGCCACG CTTCTGCACA ATATGATTGC TTACATGCTT
    ATGATGAAGG TCGGTAAAAA CGACATCAGG AAGAAAGTAC GTCGTCTTAT GGGCAAATCC
    CACATTGGCC TCACTCACAG TCAGGAGATC AATGAAGTCC TGGATCGCCT CGCACATTTA
    AGCGGTCGGG AGCTTTCTAT CAGGCCGAGT GGCAGCAGGC ATATTAAAAA ACAGACATTT
    GTTGTGCATG CTGGGACAGA TACAACTGGT GACATATTCT TCATGGAGGT GTGTGATGAT
    TGCATCGTCT TGCGCAGTAA TATTGGCACA GTCTATGAGC GCTGGTGGTA TGAGAAGCTC
    ATAAATATGA CTTACTGTCC CAAAACTAAA GTGTTGTGTC TGTGGAGGAG AAACGGCCAG
    GAGACGCAGC TAAACAAGTT TTACACTAAG AAGTGTCGGG AATTGTACTA CTGTGTTAAA
    GACAGCATGG AGCGTGCTGC TGCTCGACAA CAAAGCATCA AACCAGGTCC AGAGCTTGGA
    GGCGAGTTTC CGGTTCAGGA CATGAAGACA GGGGAGGGTG GTCTCCTCCA AGTCACACTG
    GAAGGCATCA ACCTCAAATT TATGCACAGC CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT
    CACATTAAAA AGTGCAATAC AGTGAAAGGA GTCTTTGTCC TGGAGGAGTT TGTTCCTGAA
    ACTAAAGAAG TGGTGATCCA TAAGTACAAG ACTCCTATGG CACATCAGAT CTGTTACTCG
    GTGCTCTGCC TCTTCTCCTA CATGGCGGCG GTCAAGGGGA AAGAGTCAGA GGGCAAACCC
    AAGATGCTGT CCCCTCGTCC ACTAGCGAGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa143480
    Clone ID Related Accession (Same CDS sequence) XM_021477891.1
    Accession Version XM_021477891.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5055bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA TTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGAACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCAGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT ACCCAAGCTC TGCCCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTTCCCAGC CTCAAAGACG ATGCTGATGA CAGTCCTGAA
    AGTCCTGGTG CCCGAGCCCC CAGAGCACTG GTAGATCAGA AATCCTCTGT TATTAAGCAC
    AGCCCTACTG TGAAGAGAGA GTCTCCGTCT CCTCAAGGCA GAGCCAACAA TACCAGTGAG
    AACCAGCAGT TTCTAAAGGA GGTGGTCCAG AGCGTGCTGG AGGGGCAGGG TGTGGGCTGG
    CTTAACATGA AGAAGGTGCG TCGCCTGCTA GAGAATGAAC AGCTCAGAGT CTTTGTGCTC
    AGCAAACTCA ACCGTGCGGT CCAGTCAGAG GAGGACGCCC AGCAGGAAGT TATAAGAGAT
    GTGGAGATAA ATAGAAAGGT CTACAAAGGC ATGCTGGATA TTTTGAAGTG CACGGTGTCC
    AGTTTGGAGC ATTCATACAC TAATGCTGGC TTAGGGGGCA TGGCCAGTGT CTTCAGTCTG
    CTAGAGATAG CACGCACCCA CTACCAAACC AAAGATCCAG AGAAGCGGAA ACGGAGCCCC
    ACCGAAGGGC TTAGCAGTCC AGGCAGTAAG GAGAGCCCAT CGGGCCGAAT GGAGAGTGCC
    AGGGCGGCAG GGGTGCTTCT GGTACCCCGT ATCCAATTGC CGCCACCCTC CTCTGGGAAA
    TCATCACGCC AGTTTGATAC CCGGAGTCTG AACGAGGAGA ATTTTATTGC CTCCATTGAA
    TTGTGGAGCA AGCACCAGGA TAACAGAAAG CAAAAAGCTT TGGAAAAAGA ACAGAGTGGT
    TTAAATAAAC GGAAATCAGG AGCGGATGGA GTAAAACAAC GTGTAGAAGG AGGAGACACT
    GAGGAGAAGA AATCACAGAT CAGTGCAGAC AGTGGCCTCA GTGTGACATC TGGCTCACAG
    AAGAGTGATA CGGATTCCCT GGCGAGCTCT GAACCTCCTG CTCTGACCAG AAGCACCAGC
    CAGGACTCAG AGGCCAGCAC AGTGGTAAGT AACAGCTCAG GTGAGACGCT CGGTGCAGAC
    AGTGACCTGA GCAGCACTGC TGGAGATGGC CTGACAGGAA GACATGGTCA GCATCTCAAC
    CTGTCCCGTG GCACACTGTC TGACAGTGAG ATTGAGACCA ACCCTGCAAC CAGCTCAGTG
    TTTGGCAAGA CACACAAGCT GAAGCCAGGA GTGAAGGAGC CTGTTGGTGT TAATAAAGCA
    GCACCTGCTC CACCCGTTGA GGATGTTAGC ATGAGGATCT ATCTGTGTGA GGGGCTTCTG
    GGCCGGGATA AAAGCTCAGT TTGGGACCAG TTGGAGGATG CTGCAATGGA AACCTTTTCT
    CTGAGTAAAG AGCGCTCAAC TCTGTGGGAT CAGATGCAGT TCTGGGAGGA CGCCTTCCTG
    GATGCCGTAA TGCTGGAACG GGAAGGAATG GGAATGGATC AGGGACCACA AGAAATGATT
    GACAGGTATC TTTCTCTGGG AGATCATGAC CGCAAGAGAC TGGAGGATGA TGAGGACAGA
    CTGCTGGCCA CGCTTCTGCA CAATATGATT GCTTACATGC TTATGATGAA GGTCGGTAAA
    AACGACATCA GGAAGAAAGT ACGTCGTCTT ATGGGCAAAT CCCACATTGG CCTCACTCAC
    AGTCAGGAGA TCAATGAAGT CCTGGATCGC CTCGCACATT TAAGCGGTCG GGAGCTTTCT
    ATCAGGCCGA GTGGCAGCAG GCATATTAAA AAACAGACAT TTGTTGTGCA TGCTGGGACA
    GATACAACTG GTGACATATT CTTCATGGAG GTGTGTGATG ATTGCATCGT CTTGCGCAGT
    AATATTGGCA CAGTCTATGA GCGCTGGTGG TATGAGAAGC TCATAAATAT GACTTACTGT
    CCCAAAACTA AAGTGTTGTG TCTGTGGAGG AGAAACGGCC AGGAGACGCA GCTAAACAAG
    TTTTACACTA AGAAGTGTCG GGAATTGTAC TACTGTGTTA AAGACAGCAT GGAGCGTGCT
    GCTGCTCGAC AACAAAGCAT CAAACCAGGT CCAGAGCTTG GAGGCGAGTT TCCGGTTCAG
    GACATGAAGA CAGGGGAGGG TGGTCTCCTC CAAGTCACAC TGGAAGGCAT CAACCTCAAA
    TTTATGCACA GCCAGGAGCG GAAGGTTTTC ATAGAGCTGA ATCACATTAA AAAGTGCAAT
    ACAGTGAAAG GAGTCTTTGT CCTGGAGGAG TTTGTTCCTG AAACTAAAGA AGTGGTGATC
    CATAAGTACA AGACTCCTAT GGCACATCAG ATCTGTTACT CGGTGCTCTG CCTCTTCTCC
    TACATGGCGG CGGTCAAGGG GAAAGAGTCA GAGGGCAAAC CCAAGATGCT GTCCCCTCGT
    CCACTAGCGA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333566.1
    CDS432..5486
    Translation

    Target ORF information:

    RefSeq Version XM_021477891.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021477891.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA TTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGAACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCAGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT ACCCAAGCTC TGCCCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTTCCCAGC CTCAAAGACG ATGCTGATGA CAGTCCTGAA
    AGTCCTGGTG CCCGAGCCCC CAGAGCACTG GTAGATCAGA AATCCTCTGT TATTAAGCAC
    AGCCCTACTG TGAAGAGAGA GTCTCCGTCT CCTCAAGGCA GAGCCAACAA TACCAGTGAG
    AACCAGCAGT TTCTAAAGGA GGTGGTCCAG AGCGTGCTGG AGGGGCAGGG TGTGGGCTGG
    CTTAACATGA AGAAGGTGCG TCGCCTGCTA GAGAATGAAC AGCTCAGAGT CTTTGTGCTC
    AGCAAACTCA ACCGTGCGGT CCAGTCAGAG GAGGACGCCC AGCAGGAAGT TATAAGAGAT
    GTGGAGATAA ATAGAAAGGT CTACAAAGGC ATGCTGGATA TTTTGAAGTG CACGGTGTCC
    AGTTTGGAGC ATTCATACAC TAATGCTGGC TTAGGGGGCA TGGCCAGTGT CTTCAGTCTG
    CTAGAGATAG CACGCACCCA CTACCAAACC AAAGATCCAG AGAAGCGGAA ACGGAGCCCC
    ACCGAAGGGC TTAGCAGTCC AGGCAGTAAG GAGAGCCCAT CGGGCCGAAT GGAGAGTGCC
    AGGGCGGCAG GGGTGCTTCT GGTACCCCGT ATCCAATTGC CGCCACCCTC CTCTGGGAAA
    TCATCACGCC AGTTTGATAC CCGGAGTCTG AACGAGGAGA ATTTTATTGC CTCCATTGAA
    TTGTGGAGCA AGCACCAGGA TAACAGAAAG CAAAAAGCTT TGGAAAAAGA ACAGAGTGGT
    TTAAATAAAC GGAAATCAGG AGCGGATGGA GTAAAACAAC GTGTAGAAGG AGGAGACACT
    GAGGAGAAGA AATCACAGAT CAGTGCAGAC AGTGGCCTCA GTGTGACATC TGGCTCACAG
    AAGAGTGATA CGGATTCCCT GGCGAGCTCT GAACCTCCTG CTCTGACCAG AAGCACCAGC
    CAGGACTCAG AGGCCAGCAC AGTGGTAAGT AACAGCTCAG GTGAGACGCT CGGTGCAGAC
    AGTGACCTGA GCAGCACTGC TGGAGATGGC CTGACAGGAA GACATGGTCA GCATCTCAAC
    CTGTCCCGTG GCACACTGTC TGACAGTGAG ATTGAGACCA ACCCTGCAAC CAGCTCAGTG
    TTTGGCAAGA CACACAAGCT GAAGCCAGGA GTGAAGGAGC CTGTTGGTGT TAATAAAGCA
    GCACCTGCTC CACCCGTTGA GGATGTTAGC ATGAGGATCT ATCTGTGTGA GGGGCTTCTG
    GGCCGGGATA AAAGCTCAGT TTGGGACCAG TTGGAGGATG CTGCAATGGA AACCTTTTCT
    CTGAGTAAAG AGCGCTCAAC TCTGTGGGAT CAGATGCAGT TCTGGGAGGA CGCCTTCCTG
    GATGCCGTAA TGCTGGAACG GGAAGGAATG GGAATGGATC AGGGACCACA AGAAATGATT
    GACAGGTATC TTTCTCTGGG AGATCATGAC CGCAAGAGAC TGGAGGATGA TGAGGACAGA
    CTGCTGGCCA CGCTTCTGCA CAATATGATT GCTTACATGC TTATGATGAA GGTCGGTAAA
    AACGACATCA GGAAGAAAGT ACGTCGTCTT ATGGGCAAAT CCCACATTGG CCTCACTCAC
    AGTCAGGAGA TCAATGAAGT CCTGGATCGC CTCGCACATT TAAGCGGTCG GGAGCTTTCT
    ATCAGGCCGA GTGGCAGCAG GCATATTAAA AAACAGACAT TTGTTGTGCA TGCTGGGACA
    GATACAACTG GTGACATATT CTTCATGGAG GTGTGTGATG ATTGCATCGT CTTGCGCAGT
    AATATTGGCA CAGTCTATGA GCGCTGGTGG TATGAGAAGC TCATAAATAT GACTTACTGT
    CCCAAAACTA AAGTGTTGTG TCTGTGGAGG AGAAACGGCC AGGAGACGCA GCTAAACAAG
    TTTTACACTA AGAAGTGTCG GGAATTGTAC TACTGTGTTA AAGACAGCAT GGAGCGTGCT
    GCTGCTCGAC AACAAAGCAT CAAACCAGGT CCAGAGCTTG GAGGCGAGTT TCCGGTTCAG
    GACATGAAGA CAGGGGAGGG TGGTCTCCTC CAAGTCACAC TGGAAGGCAT CAACCTCAAA
    TTTATGCACA GCCAGGAGCG GAAGGTTTTC ATAGAGCTGA ATCACATTAA AAAGTGCAAT
    ACAGTGAAAG GAGTCTTTGT CCTGGAGGAG TTTGTTCCTG AAACTAAAGA AGTGGTGATC
    CATAAGTACA AGACTCCTAT GGCACATCAG ATCTGTTACT CGGTGCTCTG CCTCTTCTCC
    TACATGGCGG CGGTCAAGGG GAAAGAGTCA GAGGGCAAAC CCAAGATGCT GTCCCCTCGT
    CCACTAGCGA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa143481
    Clone ID Related Accession (Same CDS sequence) XM_021477892.1
    Accession Version XM_021477892.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5025bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA TTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGAACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCAGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT ACCCAAGCTC TGCCCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTTCCCAGC CTCAAAGGTG CCCGAGCCCC CAGAGCACTG
    GTAGATCAGA AATCCTCTGT TATTAAGCAC AGCCCTACTG TGAAGAGAGA GTCTCCGTCT
    CCTCAAGGCA GAGCCAACAA TACCAGTGAG AACCAGCAGT TTCTAAAGGA GGTGGTCCAG
    AGCGTGCTGG AGGGGCAGGG TGTGGGCTGG CTTAACATGA AGAAGGTGCG TCGCCTGCTA
    GAGAATGAAC AGCTCAGAGT CTTTGTGCTC AGCAAACTCA ACCGTGCGGT CCAGTCAGAG
    GAGGACGCCC AGCAGGAAGT TATAAGAGAT GTGGAGATAA ATAGAAAGGT CTACAAAGGC
    ATGCTGGATA TTTTGAAGTG CACGGTGTCC AGTTTGGAGC ATTCATACAC TAATGCTGGC
    TTAGGGGGCA TGGCCAGTGT CTTCAGTCTG CTAGAGATAG CACGCACCCA CTACCAAACC
    AAAGATCCAG AGAAGCGGAA ACGGAGCCCC ACCGAAGGGC TTAGCAGTCC AGGCAGTAAG
    GAGAGCCCAT CGGGCCGAAT GGAGAGTGCC AGGGCGGCAG GGGTGCTTCT GGTACCCCGT
    ATCCAATTGC CGCCACCCTC CTCTGGGAAA TCATCACGCC AGTTTGATAC CCGGAGTCTG
    AACGAGGAGA ATTTTATTGC CTCCATTGAA TTGTGGAGCA AGCACCAGGA TAACAGAAAG
    CAAAAAGCTT TGGAAAAAGA ACAGAGTGGT TTAAATAAAC GGAAATCAGG AGCGGATGGA
    GTAAAACAAC GTGTAGAAGG AGGAGACACT GAGGAGAAGA AATCACAGAT CAGTGCAGAC
    AGTGGCCTCA GTGTGACATC TGGCTCACAG AAGAGTGATA CGGATTCCCT GGCGAGCTCT
    GAACCTCCTG CTCTGACCAG AAGCACCAGC CAGGACTCAG AGGCCAGCAC AGTGGTAAGT
    AACAGCTCAG GTGAGACGCT CGGTGCAGAC AGTGACCTGA GCAGCACTGC TGGAGATGGC
    CTGACAGGAA GACATGGTCA GCATCTCAAC CTGTCCCGTG GCACACTGTC TGACAGTGAG
    ATTGAGACCA ACCCTGCAAC CAGCTCAGTG TTTGGCAAGA CACACAAGCT GAAGCCAGGA
    GTGAAGGAGC CTGTTGGTGT TAATAAAGCA GCACCTGCTC CACCCGTTGA GGATGTTAGC
    ATGAGGATCT ATCTGTGTGA GGGGCTTCTG GGCCGGGATA AAAGCTCAGT TTGGGACCAG
    TTGGAGGATG CTGCAATGGA AACCTTTTCT CTGAGTAAAG AGCGCTCAAC TCTGTGGGAT
    CAGATGCAGT TCTGGGAGGA CGCCTTCCTG GATGCCGTAA TGCTGGAACG GGAAGGAATG
    GGAATGGATC AGGGACCACA AGAAATGATT GACAGGTATC TTTCTCTGGG AGATCATGAC
    CGCAAGAGAC TGGAGGATGA TGAGGACAGA CTGCTGGCCA CGCTTCTGCA CAATATGATT
    GCTTACATGC TTATGATGAA GGTCGGTAAA AACGACATCA GGAAGAAAGT ACGTCGTCTT
    ATGGGCAAAT CCCACATTGG CCTCACTCAC AGTCAGGAGA TCAATGAAGT CCTGGATCGC
    CTCGCACATT TAAGCGGTCG GGAGCTTTCT ATCAGGCCGA GTGGCAGCAG GCATATTAAA
    AAACAGACAT TTGTTGTGCA TGCTGGGACA GATACAACTG GTGACATATT CTTCATGGAG
    GTGTGTGATG ATTGCATCGT CTTGCGCAGT AATATTGGCA CAGTCTATGA GCGCTGGTGG
    TATGAGAAGC TCATAAATAT GACTTACTGT CCCAAAACTA AAGTGTTGTG TCTGTGGAGG
    AGAAACGGCC AGGAGACGCA GCTAAACAAG TTTTACACTA AGAAGTGTCG GGAATTGTAC
    TACTGTGTTA AAGACAGCAT GGAGCGTGCT GCTGCTCGAC AACAAAGCAT CAAACCAGGT
    CCAGAGCTTG GAGGCGAGTT TCCGGTTCAG GACATGAAGA CAGGGGAGGG TGGTCTCCTC
    CAAGTCACAC TGGAAGGCAT CAACCTCAAA TTTATGCACA GCCAGGAGCG GAAGGTTTTC
    ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTGAAAG GAGTCTTTGT CCTGGAGGAG
    TTTGTTCCTG AAACTAAAGA AGTGGTGATC CATAAGTACA AGACTCCTAT GGCACATCAG
    ATCTGTTACT CGGTGCTCTG CCTCTTCTCC TACATGGCGG CGGTCAAGGG GAAAGAGTCA
    GAGGGCAAAC CCAAGATGCT GTCCCCTCGT CCACTAGCGA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333567.1
    CDS432..5456
    Translation

    Target ORF information:

    RefSeq Version XM_021477892.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021477892.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA TTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGAACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCAGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT ACCCAAGCTC TGCCCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTTCCCAGC CTCAAAGGTG CCCGAGCCCC CAGAGCACTG
    GTAGATCAGA AATCCTCTGT TATTAAGCAC AGCCCTACTG TGAAGAGAGA GTCTCCGTCT
    CCTCAAGGCA GAGCCAACAA TACCAGTGAG AACCAGCAGT TTCTAAAGGA GGTGGTCCAG
    AGCGTGCTGG AGGGGCAGGG TGTGGGCTGG CTTAACATGA AGAAGGTGCG TCGCCTGCTA
    GAGAATGAAC AGCTCAGAGT CTTTGTGCTC AGCAAACTCA ACCGTGCGGT CCAGTCAGAG
    GAGGACGCCC AGCAGGAAGT TATAAGAGAT GTGGAGATAA ATAGAAAGGT CTACAAAGGC
    ATGCTGGATA TTTTGAAGTG CACGGTGTCC AGTTTGGAGC ATTCATACAC TAATGCTGGC
    TTAGGGGGCA TGGCCAGTGT CTTCAGTCTG CTAGAGATAG CACGCACCCA CTACCAAACC
    AAAGATCCAG AGAAGCGGAA ACGGAGCCCC ACCGAAGGGC TTAGCAGTCC AGGCAGTAAG
    GAGAGCCCAT CGGGCCGAAT GGAGAGTGCC AGGGCGGCAG GGGTGCTTCT GGTACCCCGT
    ATCCAATTGC CGCCACCCTC CTCTGGGAAA TCATCACGCC AGTTTGATAC CCGGAGTCTG
    AACGAGGAGA ATTTTATTGC CTCCATTGAA TTGTGGAGCA AGCACCAGGA TAACAGAAAG
    CAAAAAGCTT TGGAAAAAGA ACAGAGTGGT TTAAATAAAC GGAAATCAGG AGCGGATGGA
    GTAAAACAAC GTGTAGAAGG AGGAGACACT GAGGAGAAGA AATCACAGAT CAGTGCAGAC
    AGTGGCCTCA GTGTGACATC TGGCTCACAG AAGAGTGATA CGGATTCCCT GGCGAGCTCT
    GAACCTCCTG CTCTGACCAG AAGCACCAGC CAGGACTCAG AGGCCAGCAC AGTGGTAAGT
    AACAGCTCAG GTGAGACGCT CGGTGCAGAC AGTGACCTGA GCAGCACTGC TGGAGATGGC
    CTGACAGGAA GACATGGTCA GCATCTCAAC CTGTCCCGTG GCACACTGTC TGACAGTGAG
    ATTGAGACCA ACCCTGCAAC CAGCTCAGTG TTTGGCAAGA CACACAAGCT GAAGCCAGGA
    GTGAAGGAGC CTGTTGGTGT TAATAAAGCA GCACCTGCTC CACCCGTTGA GGATGTTAGC
    ATGAGGATCT ATCTGTGTGA GGGGCTTCTG GGCCGGGATA AAAGCTCAGT TTGGGACCAG
    TTGGAGGATG CTGCAATGGA AACCTTTTCT CTGAGTAAAG AGCGCTCAAC TCTGTGGGAT
    CAGATGCAGT TCTGGGAGGA CGCCTTCCTG GATGCCGTAA TGCTGGAACG GGAAGGAATG
    GGAATGGATC AGGGACCACA AGAAATGATT GACAGGTATC TTTCTCTGGG AGATCATGAC
    CGCAAGAGAC TGGAGGATGA TGAGGACAGA CTGCTGGCCA CGCTTCTGCA CAATATGATT
    GCTTACATGC TTATGATGAA GGTCGGTAAA AACGACATCA GGAAGAAAGT ACGTCGTCTT
    ATGGGCAAAT CCCACATTGG CCTCACTCAC AGTCAGGAGA TCAATGAAGT CCTGGATCGC
    CTCGCACATT TAAGCGGTCG GGAGCTTTCT ATCAGGCCGA GTGGCAGCAG GCATATTAAA
    AAACAGACAT TTGTTGTGCA TGCTGGGACA GATACAACTG GTGACATATT CTTCATGGAG
    GTGTGTGATG ATTGCATCGT CTTGCGCAGT AATATTGGCA CAGTCTATGA GCGCTGGTGG
    TATGAGAAGC TCATAAATAT GACTTACTGT CCCAAAACTA AAGTGTTGTG TCTGTGGAGG
    AGAAACGGCC AGGAGACGCA GCTAAACAAG TTTTACACTA AGAAGTGTCG GGAATTGTAC
    TACTGTGTTA AAGACAGCAT GGAGCGTGCT GCTGCTCGAC AACAAAGCAT CAAACCAGGT
    CCAGAGCTTG GAGGCGAGTT TCCGGTTCAG GACATGAAGA CAGGGGAGGG TGGTCTCCTC
    CAAGTCACAC TGGAAGGCAT CAACCTCAAA TTTATGCACA GCCAGGAGCG GAAGGTTTTC
    ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTGAAAG GAGTCTTTGT CCTGGAGGAG
    TTTGTTCCTG AAACTAAAGA AGTGGTGATC CATAAGTACA AGACTCCTAT GGCACATCAG
    ATCTGTTACT CGGTGCTCTG CCTCTTCTCC TACATGGCGG CGGTCAAGGG GAAAGAGTCA
    GAGGGCAAAC CCAAGATGCT GTCCCCTCGT CCACTAGCGA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa143482
    Clone ID Related Accession (Same CDS sequence) XM_021477894.1
    Accession Version XM_021477894.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4944bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA TTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGAACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCAGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT ACCCAAGCTC TGCCCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTTCCCAGC CTCAAAGGTG CCCGAGCCCC CAGAGCACTG
    GTAGATCAGA AATCCTCTGT TATTAAGCAC AGCCCTACTG TGAAGAGAGA GTCTCCGTCT
    CCTCAAGGCA GAGCCAACAA TACCAGTGAG AACCAGCAGT TTCTAAAGGA GGTGGTCCAG
    AGCGTGCTGG AGGGGCAGGG TGTGGGCTGG CTTAACATGA AGAAGGTGCG TCGCCTGCTA
    GAGAATGAAC AGCTCAGAGT CTTTGTGCTC AGCAAACTCA ACCGTGCGGT CCAGTCAGAG
    GAGGACGCCC AGCAGGAAGT TATAAGAGAT GTGGAGATAA ATAGAAAGGT CTACAAAGGC
    ATGCTGGATA TTTTGAAGTG CACGGTGTCC AGTTTGGAGC ATTCATACAC TAATGCTGGC
    TTAGGGGGCA TGGCCAGTGT CTTCAGTCTG CTAGAGATAG CACGCACCCA CTACCAAACC
    AAAGATCCAG AGAAGCGGAA ACGGAGCCCC ACCGAAGGGC TTAGCAGTCC AGGCAGTAAG
    GAGAGCCCAT CGGGCCGAAT GGAGAGTGCC AGGGCGGCAG GGGTGCTTCT GGTACCCCGT
    ATCCAATTGC CGCCACCCTC CTCTGGGAAA TCATCACGCC AGTTTGATAC CCGGAGTCTG
    AACGAGGAGA ATTTTATTGC CTCCATTGGA GCGGATGGAG TAAAACAACG TGTAGAAGGA
    GGAGACACTG AGGAGAAGAA ATCACAGATC AGTGCAGACA GTGGCCTCAG TGTGACATCT
    GGCTCACAGA AGAGTGATAC GGATTCCCTG GCGAGCTCTG AACCTCCTGC TCTGACCAGA
    AGCACCAGCC AGGACTCAGA GGCCAGCACA GTGGTAAGTA ACAGCTCAGG TGAGACGCTC
    GGTGCAGACA GTGACCTGAG CAGCACTGCT GGAGATGGCC TGACAGGAAG ACATGGTCAG
    CATCTCAACC TGTCCCGTGG CACACTGTCT GACAGTGAGA TTGAGACCAA CCCTGCAACC
    AGCTCAGTGT TTGGCAAGAC ACACAAGCTG AAGCCAGGAG TGAAGGAGCC TGTTGGTGTT
    AATAAAGCAG CACCTGCTCC ACCCGTTGAG GATGTTAGCA TGAGGATCTA TCTGTGTGAG
    GGGCTTCTGG GCCGGGATAA AAGCTCAGTT TGGGACCAGT TGGAGGATGC TGCAATGGAA
    ACCTTTTCTC TGAGTAAAGA GCGCTCAACT CTGTGGGATC AGATGCAGTT CTGGGAGGAC
    GCCTTCCTGG ATGCCGTAAT GCTGGAACGG GAAGGAATGG GAATGGATCA GGGACCACAA
    GAAATGATTG ACAGGTATCT TTCTCTGGGA GATCATGACC GCAAGAGACT GGAGGATGAT
    GAGGACAGAC TGCTGGCCAC GCTTCTGCAC AATATGATTG CTTACATGCT TATGATGAAG
    GTCGGTAAAA ACGACATCAG GAAGAAAGTA CGTCGTCTTA TGGGCAAATC CCACATTGGC
    CTCACTCACA GTCAGGAGAT CAATGAAGTC CTGGATCGCC TCGCACATTT AAGCGGTCGG
    GAGCTTTCTA TCAGGCCGAG TGGCAGCAGG CATATTAAAA AACAGACATT TGTTGTGCAT
    GCTGGGACAG ATACAACTGG TGACATATTC TTCATGGAGG TGTGTGATGA TTGCATCGTC
    TTGCGCAGTA ATATTGGCAC AGTCTATGAG CGCTGGTGGT ATGAGAAGCT CATAAATATG
    ACTTACTGTC CCAAAACTAA AGTGTTGTGT CTGTGGAGGA GAAACGGCCA GGAGACGCAG
    CTAAACAAGT TTTACACTAA GAAGTGTCGG GAATTGTACT ACTGTGTTAA AGACAGCATG
    GAGCGTGCTG CTGCTCGACA ACAAAGCATC AAACCAGGTC CAGAGCTTGG AGGCGAGTTT
    CCGGTTCAGG ACATGAAGAC AGGGGAGGGT GGTCTCCTCC AAGTCACACT GGAAGGCATC
    AACCTCAAAT TTATGCACAG CCAGGAGCGG AAGGTTTTCA TAGAGCTGAA TCACATTAAA
    AAGTGCAATA CAGTGAAAGG AGTCTTTGTC CTGGAGGAGT TTGTTCCTGA AACTAAAGAA
    GTGGTGATCC ATAAGTACAA GACTCCTATG GCACATCAGA TCTGTTACTC GGTGCTCTGC
    CTCTTCTCCT ACATGGCGGC GGTCAAGGGG AAAGAGTCAG AGGGCAAACC CAAGATGCTG
    TCCCCTCGTC CACTAGCGAG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333569.1
    CDS432..5375
    Translation

    Target ORF information:

    RefSeq Version XM_021477894.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X25, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021477894.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA TTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGAACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCAGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT ACCCAAGCTC TGCCCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTTCCCAGC CTCAAAGGTG CCCGAGCCCC CAGAGCACTG
    GTAGATCAGA AATCCTCTGT TATTAAGCAC AGCCCTACTG TGAAGAGAGA GTCTCCGTCT
    CCTCAAGGCA GAGCCAACAA TACCAGTGAG AACCAGCAGT TTCTAAAGGA GGTGGTCCAG
    AGCGTGCTGG AGGGGCAGGG TGTGGGCTGG CTTAACATGA AGAAGGTGCG TCGCCTGCTA
    GAGAATGAAC AGCTCAGAGT CTTTGTGCTC AGCAAACTCA ACCGTGCGGT CCAGTCAGAG
    GAGGACGCCC AGCAGGAAGT TATAAGAGAT GTGGAGATAA ATAGAAAGGT CTACAAAGGC
    ATGCTGGATA TTTTGAAGTG CACGGTGTCC AGTTTGGAGC ATTCATACAC TAATGCTGGC
    TTAGGGGGCA TGGCCAGTGT CTTCAGTCTG CTAGAGATAG CACGCACCCA CTACCAAACC
    AAAGATCCAG AGAAGCGGAA ACGGAGCCCC ACCGAAGGGC TTAGCAGTCC AGGCAGTAAG
    GAGAGCCCAT CGGGCCGAAT GGAGAGTGCC AGGGCGGCAG GGGTGCTTCT GGTACCCCGT
    ATCCAATTGC CGCCACCCTC CTCTGGGAAA TCATCACGCC AGTTTGATAC CCGGAGTCTG
    AACGAGGAGA ATTTTATTGC CTCCATTGGA GCGGATGGAG TAAAACAACG TGTAGAAGGA
    GGAGACACTG AGGAGAAGAA ATCACAGATC AGTGCAGACA GTGGCCTCAG TGTGACATCT
    GGCTCACAGA AGAGTGATAC GGATTCCCTG GCGAGCTCTG AACCTCCTGC TCTGACCAGA
    AGCACCAGCC AGGACTCAGA GGCCAGCACA GTGGTAAGTA ACAGCTCAGG TGAGACGCTC
    GGTGCAGACA GTGACCTGAG CAGCACTGCT GGAGATGGCC TGACAGGAAG ACATGGTCAG
    CATCTCAACC TGTCCCGTGG CACACTGTCT GACAGTGAGA TTGAGACCAA CCCTGCAACC
    AGCTCAGTGT TTGGCAAGAC ACACAAGCTG AAGCCAGGAG TGAAGGAGCC TGTTGGTGTT
    AATAAAGCAG CACCTGCTCC ACCCGTTGAG GATGTTAGCA TGAGGATCTA TCTGTGTGAG
    GGGCTTCTGG GCCGGGATAA AAGCTCAGTT TGGGACCAGT TGGAGGATGC TGCAATGGAA
    ACCTTTTCTC TGAGTAAAGA GCGCTCAACT CTGTGGGATC AGATGCAGTT CTGGGAGGAC
    GCCTTCCTGG ATGCCGTAAT GCTGGAACGG GAAGGAATGG GAATGGATCA GGGACCACAA
    GAAATGATTG ACAGGTATCT TTCTCTGGGA GATCATGACC GCAAGAGACT GGAGGATGAT
    GAGGACAGAC TGCTGGCCAC GCTTCTGCAC AATATGATTG CTTACATGCT TATGATGAAG
    GTCGGTAAAA ACGACATCAG GAAGAAAGTA CGTCGTCTTA TGGGCAAATC CCACATTGGC
    CTCACTCACA GTCAGGAGAT CAATGAAGTC CTGGATCGCC TCGCACATTT AAGCGGTCGG
    GAGCTTTCTA TCAGGCCGAG TGGCAGCAGG CATATTAAAA AACAGACATT TGTTGTGCAT
    GCTGGGACAG ATACAACTGG TGACATATTC TTCATGGAGG TGTGTGATGA TTGCATCGTC
    TTGCGCAGTA ATATTGGCAC AGTCTATGAG CGCTGGTGGT ATGAGAAGCT CATAAATATG
    ACTTACTGTC CCAAAACTAA AGTGTTGTGT CTGTGGAGGA GAAACGGCCA GGAGACGCAG
    CTAAACAAGT TTTACACTAA GAAGTGTCGG GAATTGTACT ACTGTGTTAA AGACAGCATG
    GAGCGTGCTG CTGCTCGACA ACAAAGCATC AAACCAGGTC CAGAGCTTGG AGGCGAGTTT
    CCGGTTCAGG ACATGAAGAC AGGGGAGGGT GGTCTCCTCC AAGTCACACT GGAAGGCATC
    AACCTCAAAT TTATGCACAG CCAGGAGCGG AAGGTTTTCA TAGAGCTGAA TCACATTAAA
    AAGTGCAATA CAGTGAAAGG AGTCTTTGTC CTGGAGGAGT TTGTTCCTGA AACTAAAGAA
    GTGGTGATCC ATAAGTACAA GACTCCTATG GCACATCAGA TCTGTTACTC GGTGCTCTGC
    CTCTTCTCCT ACATGGCGGC GGTCAAGGGG AAAGAGTCAG AGGGCAAACC CAAGATGCTG
    TCCCCTCGTC CACTAGCGAG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa143483
    Clone ID Related Accession (Same CDS sequence) XM_021477895.1
    Accession Version XM_021477895.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4935bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA TTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGAACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCAGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT ACCCAAGCTC TGCCCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTTCCCAGC CTCAAAGAAT ATTTTAATTT TGATTTAGCA
    GAGGACACAG ATCAAGACGA TGCTGATGAC AGTCCTGAAA GTCCTGTATT TGGGCTAAAT
    TCTCTAATGG AGATTGTAAC AGAGATCGGC CCGGGGAGCG GAGAAGGTGC CCGAGCCCCC
    AGAGCACTGG TAGATCAGAA ATCCTCTGTT ATTAAGCACA GCCCTACTGT GAAGAGAGAG
    TCTCCGTCTC CTCAAGGCAG AGCCAACAAT ACCAGTGAGA ACCAGCAGTT TCTAAAGGAG
    GTGGTCCAGA GCGTGCTGGA GGGGCAGGGT GTGGGCTGGC TTAACATGAA GAAGGTGCGT
    CGCCTGCTAG AGAATGAACA GCTCAGAGTC TTTGTGCTCA GCAAACTCAA CCGTGCGGTC
    CAGTCAGAGG AGGACGCCCA GCAGGAAGTT ATAAGAGATG TGGAGATAAA TAGAAAGGTC
    TACAAAGGCA TGCTGGATAT TTTGAAGTGC ACGGTGTCCA GTTTGGAGCA TTCATACACT
    AATGCTGGCT TAGGGGGCAT GGCCAGTGTC TTCAGTCTGC TAGAGATAGC ACGCACCCAC
    TACCAAACCA AAGATCCAGA GAAGCGGAAA CGGAGCCCCA CCGAAGGGCT TAGCAGTCCA
    GGCAGTAAGG AGAGCCCATC GGGCCGAATG GAGAGTGCCA GGGCGGCAGG GGTGCTTCTG
    GTACCCCGTA TCCAATTGCC GCCACCCTCC TCTGGGAAAT CATCACGCCA GTTTGATACC
    CGGAGTCTGA ACGAGGAGAA TTTTATTGCC TCCATTGAAT TGTGGAGCAA GCACCAGGAT
    AACAGAAAGC AAAAAGCTTT GGAAAAAGAA CAGAGTGGTT TAAATAAACG GAAATCAGGA
    GCGGATGGAG TAAAACAACG TGTAGAAGGA GGAGACACTG AGGAGAAGAA ATCACAGATC
    AGTGCAGACA GTGGCCTCAG TGTGACATCT GGCTCACAGA AGAGTGATAC GGATTCCCTG
    GCGAGCTCTG AACCTCCTGC TCTGACCAGA AGCACCAGCC AGGACTCAGA GGCCAGCACA
    GTAAGTAACA GCTCAGGTGA GACGCTCGGT GCAGACAGTG ACCTGAGCAG CACTGCTGGA
    GATGGCCTGA CAGGAAGACA TGGTCAGCAT CTCAACCTGT CCCGTGGCAC ACTGTCTGAC
    AGTGAGATTG AGACCAACCC TGCAACCAGC TCAGTGTTTG GCAAGACACA CAAGCTGAAG
    CCAGGAGTGA AGGAGCCTGT TGGTGTTAAT AAAGCAGCAC CTGCTCCACC CGTTGAGGAT
    GTTAGCATGA GGATCTATCT GTGTGAGGGG CTTCTGGGCC GGGATAAAAG CTCAGTTTGG
    GACCAGTTGG AGGATGCTGC AATGGAAACC TTTTCTCTGA GTAAAGAGCG CTCAACTCTG
    TGGGATCAGA TGCAGTTCTG GGAGGACGCC TTCCTGGATG CCGTAATGCT GGAACGGGAA
    GGAATGGGAA TGGATCAGGG ACCACAAGAA ATGATTGACA GGTATCTTTC TCTGGGAGAT
    CATGACCGCA AGAGACTGGA GGATGATGAG GACAGACTGC TGGCCACGCT TCTGCACAAT
    ATGATTGCTT ACATGCTTAT GATGAAGGTC GGTAAAAACG ACATCAGGAA GAAAGTACGT
    CGTCTTATGG GCAAATCCCA CATTGGCCTC ACTCACAGTC AGGAGATCAA TGAAGTCCTG
    GATCGCCTCG CACATTTAAG CGGTCGGGAG CTTTCTATCA GGCCGAGTGG CAGCAGGCAT
    ATTAAAAAAC AGACATTTGT TGTGCATGCT GGGACAGATA CAACTGGTGA CATATTCTTC
    ATGGAGGTGT GTGATGATTG CATCGTCTTG CGCAGTAATA TTGGCACAGT CTATGAGCGC
    TGGTGGTATG AGAAGCTCAT AAATATGACT TACTGTCCCA AAACTAAAGT GTTGTGTCTG
    TGGAGGAGAA ACGGCCAGGA GACGCAGCTA AACAAGTTTT ACACTAAGAA GTGTCGGGAA
    TTGTACTACT GTGTTAAAGA CAGCATGGAG CGTGCTGCTG CTCGACAACA AAGCATCAAA
    CCAGGTCCAG AGCTTGGAGG CGAGTTTCCG GTTCAGGACA TGAAGACAGG GGAGGGTGGT
    CTCCTCCAAG TCACACTGGA AGGCATCAAC CTCAAATTTA TGCACAGCCA GTTCCTGAAA
    CTAAAGAAGT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333570.1
    CDS432..5366
    Translation

    Target ORF information:

    RefSeq Version XM_021477895.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X26, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021477895.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA TTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGAACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCAGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT ACCCAAGCTC TGCCCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTTCCCAGC CTCAAAGAAT ATTTTAATTT TGATTTAGCA
    GAGGACACAG ATCAAGACGA TGCTGATGAC AGTCCTGAAA GTCCTGTATT TGGGCTAAAT
    TCTCTAATGG AGATTGTAAC AGAGATCGGC CCGGGGAGCG GAGAAGGTGC CCGAGCCCCC
    AGAGCACTGG TAGATCAGAA ATCCTCTGTT ATTAAGCACA GCCCTACTGT GAAGAGAGAG
    TCTCCGTCTC CTCAAGGCAG AGCCAACAAT ACCAGTGAGA ACCAGCAGTT TCTAAAGGAG
    GTGGTCCAGA GCGTGCTGGA GGGGCAGGGT GTGGGCTGGC TTAACATGAA GAAGGTGCGT
    CGCCTGCTAG AGAATGAACA GCTCAGAGTC TTTGTGCTCA GCAAACTCAA CCGTGCGGTC
    CAGTCAGAGG AGGACGCCCA GCAGGAAGTT ATAAGAGATG TGGAGATAAA TAGAAAGGTC
    TACAAAGGCA TGCTGGATAT TTTGAAGTGC ACGGTGTCCA GTTTGGAGCA TTCATACACT
    AATGCTGGCT TAGGGGGCAT GGCCAGTGTC TTCAGTCTGC TAGAGATAGC ACGCACCCAC
    TACCAAACCA AAGATCCAGA GAAGCGGAAA CGGAGCCCCA CCGAAGGGCT TAGCAGTCCA
    GGCAGTAAGG AGAGCCCATC GGGCCGAATG GAGAGTGCCA GGGCGGCAGG GGTGCTTCTG
    GTACCCCGTA TCCAATTGCC GCCACCCTCC TCTGGGAAAT CATCACGCCA GTTTGATACC
    CGGAGTCTGA ACGAGGAGAA TTTTATTGCC TCCATTGAAT TGTGGAGCAA GCACCAGGAT
    AACAGAAAGC AAAAAGCTTT GGAAAAAGAA CAGAGTGGTT TAAATAAACG GAAATCAGGA
    GCGGATGGAG TAAAACAACG TGTAGAAGGA GGAGACACTG AGGAGAAGAA ATCACAGATC
    AGTGCAGACA GTGGCCTCAG TGTGACATCT GGCTCACAGA AGAGTGATAC GGATTCCCTG
    GCGAGCTCTG AACCTCCTGC TCTGACCAGA AGCACCAGCC AGGACTCAGA GGCCAGCACA
    GTAAGTAACA GCTCAGGTGA GACGCTCGGT GCAGACAGTG ACCTGAGCAG CACTGCTGGA
    GATGGCCTGA CAGGAAGACA TGGTCAGCAT CTCAACCTGT CCCGTGGCAC ACTGTCTGAC
    AGTGAGATTG AGACCAACCC TGCAACCAGC TCAGTGTTTG GCAAGACACA CAAGCTGAAG
    CCAGGAGTGA AGGAGCCTGT TGGTGTTAAT AAAGCAGCAC CTGCTCCACC CGTTGAGGAT
    GTTAGCATGA GGATCTATCT GTGTGAGGGG CTTCTGGGCC GGGATAAAAG CTCAGTTTGG
    GACCAGTTGG AGGATGCTGC AATGGAAACC TTTTCTCTGA GTAAAGAGCG CTCAACTCTG
    TGGGATCAGA TGCAGTTCTG GGAGGACGCC TTCCTGGATG CCGTAATGCT GGAACGGGAA
    GGAATGGGAA TGGATCAGGG ACCACAAGAA ATGATTGACA GGTATCTTTC TCTGGGAGAT
    CATGACCGCA AGAGACTGGA GGATGATGAG GACAGACTGC TGGCCACGCT TCTGCACAAT
    ATGATTGCTT ACATGCTTAT GATGAAGGTC GGTAAAAACG ACATCAGGAA GAAAGTACGT
    CGTCTTATGG GCAAATCCCA CATTGGCCTC ACTCACAGTC AGGAGATCAA TGAAGTCCTG
    GATCGCCTCG CACATTTAAG CGGTCGGGAG CTTTCTATCA GGCCGAGTGG CAGCAGGCAT
    ATTAAAAAAC AGACATTTGT TGTGCATGCT GGGACAGATA CAACTGGTGA CATATTCTTC
    ATGGAGGTGT GTGATGATTG CATCGTCTTG CGCAGTAATA TTGGCACAGT CTATGAGCGC
    TGGTGGTATG AGAAGCTCAT AAATATGACT TACTGTCCCA AAACTAAAGT GTTGTGTCTG
    TGGAGGAGAA ACGGCCAGGA GACGCAGCTA AACAAGTTTT ACACTAAGAA GTGTCGGGAA
    TTGTACTACT GTGTTAAAGA CAGCATGGAG CGTGCTGCTG CTCGACAACA AAGCATCAAA
    CCAGGTCCAG AGCTTGGAGG CGAGTTTCCG GTTCAGGACA TGAAGACAGG GGAGGGTGGT
    CTCCTCCAAG TCACACTGGA AGGCATCAAC CTCAAATTTA TGCACAGCCA GTTCCTGAAA
    CTAAAGAAGT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa143484
    Clone ID Related Accession (Same CDS sequence) XM_021477896.1
    Accession Version XM_021477896.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4932bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA TTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGAACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCAGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT ACCCAAGCTC TGCCCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTTCCCAGC CTCAAAGTAT TTGGGCTAAA TTCTCTAATG
    GAGATTGTAA CAGAGATCGG CCCGGGGAGC GGAGAAGGTG CCCGAGCCCC CAGAGCACTG
    GTAGATCAGA AATCCTCTGT TATTAAGCAC AGCCCTACTG TGAAGAGAGA GTCTCCGTCT
    CCTCAAGGCA GAGCCAACAA TACCAGTGAG AACCAGCAGT TTCTAAAGGA GGTGGTCCAG
    AGCGTGCTGG AGGGGCAGGG TGTGGGCTGG CTTAACATGA AGAAGGTGCG TCGCCTGCTA
    GAGAATGAAC AGCTCAGAGT CTTTGTGCTC AGCAAACTCA ACCGTGCGGT CCAGTCAGAG
    GAGGACGCCC AGCAGGAAGT TATAAGAGAT GTGGAGATAA ATAGAAAGGT CTACAAAGGC
    ATGCTGGATA TTTTGAAGTG CACGGTGTCC AGTTTGGAGC ATTCATACAC TAATGCTGGC
    TTAGGGGGCA TGGCCAGTGT CTTCAGTCTG CTAGAGATAG CACGCACCCA CTACCAAACC
    AAAGATCCAG AGAAGCGGAA ACGGAGCCCC ACCGAAGGGC TTAGCAGTCC AGGCAGTAAG
    GAGAGCCCAT CGGGCCGAAT GGAGAGTGCC AGGGCGGCAG GGGTGCTTCT GGTACCCCGT
    ATCCAATTGC CGCCACCCTC CTCTGGGAAA TCATCACGCC AGTTTGATAC CCGGAGTCTG
    AACGAGGAGA ATTTTATTGC CTCCATTGGA GCGGATGGAG TAAAACAACG TGTAGAAGGA
    GGAGACACTG AGGAGAAGAA ATCACAGATC AGTGCAGACA GTGGCCTCAG TGTGACATCT
    GGCTCACAGA AGAGTGATAC GGATTCCCTG GCGAGCTCTG AACCTCCTGC TCTGACCAGA
    AGCACCAGCC AGGACTCAGA GGCCAGCACA GTGGTAAGTA ACAGCTCAGG TGAGACGCTC
    GGTGCAGACA GTGACCTGAG CAGCACTGCT GGAGATGGCC TGACAGGAAG ACATGGTCAG
    CATCTCAACC TGTCCCGTGG CACACTGTCT GACAGTGAGA TTGAGACCAA CCCTGCAACC
    AGCTCAGTGT TTGGCAAGAC ACACAAGCTG AAGCCAGGAG TGAAGGAGCC TGTTGGTGTT
    AATAAAGCAG CACCTGCTCC ACCCGTTGAG GATGTTAGCA TGAGGATCTA TCTGTGTGAG
    GGGCTTCTGG GTAAAGAGCG CTCAACTCTG TGGGATCAGA TGCAGTTCTG GGAGGACGCC
    TTCCTGGATG CCGTAATGCT GGAACGGGAA GGAATGGGAA TGGATCAGGG ACCACAAGAA
    ATGATTGACA GGTATCTTTC TCTGGGAGAT CATGACCGCA AGAGACTGGA GGATGATGAG
    GACAGACTGC TGGCCACGCT TCTGCACAAT ATGATTGCTT ACATGCTTAT GATGAAGGTC
    GGTAAAAACG ACATCAGGAA GAAAGTACGT CGTCTTATGG GCAAATCCCA CATTGGCCTC
    ACTCACAGTC AGGAGATCAA TGAAGTCCTG GATCGCCTCG CACATTTAAG CGGTCGGGAG
    CTTTCTATCA GGCCGAGTGG CAGCAGGCAT ATTAAAAAAC AGACATTTGT TGTGCATGCT
    GGGACAGATA CAACTGGTGA CATATTCTTC ATGGAGGTGT GTGATGATTG CATCGTCTTG
    CGCAGTAATA TTGGCACAGT CTATGAGCGC TGGTGGTATG AGAAGCTCAT AAATATGACT
    TACTGTCCCA AAACTAAAGT GTTGTGTCTG TGGAGGAGAA ACGGCCAGGA GACGCAGCTA
    AACAAGTTTT ACACTAAGAA GTGTCGGGAA TTGTACTACT GTGTTAAAGA CAGCATGGAG
    CGTGCTGCTG CTCGACAACA AAGCATCAAA CCAGGTCCAG AGCTTGGAGG CGAGTTTCCG
    GTTCAGGACA TGAAGACAGG GGAGGGTGGT CTCCTCCAAG TCACACTGGA AGGCATCAAC
    CTCAAATTTA TGCACAGCCA GGTTTTCATA GAGCTGAATC ACATTAAAAA GTGCAATACA
    GTGAAAGGAG TCTTTGTCCT GGAGGAGTTT GTTCCTGAAA CTAAAGAAGT GGTGATCCAT
    AAGTACAAGA CTCCTATGGC ACATCAGATC TGTTACTCGG TGCTCTGCCT CTTCTCCTAC
    ATGGCGGCGG TCAAGGGGAA AGAGTCAGAG GGCAAACCCA AGATGCTGTC CCCTCGTCCA
    CTAGCGAGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333571.1
    CDS432..5363
    Translation

    Target ORF information:

    RefSeq Version XM_021477896.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X27, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021477896.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA TTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGAACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCAGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT ACCCAAGCTC TGCCCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTTCCCAGC CTCAAAGTAT TTGGGCTAAA TTCTCTAATG
    GAGATTGTAA CAGAGATCGG CCCGGGGAGC GGAGAAGGTG CCCGAGCCCC CAGAGCACTG
    GTAGATCAGA AATCCTCTGT TATTAAGCAC AGCCCTACTG TGAAGAGAGA GTCTCCGTCT
    CCTCAAGGCA GAGCCAACAA TACCAGTGAG AACCAGCAGT TTCTAAAGGA GGTGGTCCAG
    AGCGTGCTGG AGGGGCAGGG TGTGGGCTGG CTTAACATGA AGAAGGTGCG TCGCCTGCTA
    GAGAATGAAC AGCTCAGAGT CTTTGTGCTC AGCAAACTCA ACCGTGCGGT CCAGTCAGAG
    GAGGACGCCC AGCAGGAAGT TATAAGAGAT GTGGAGATAA ATAGAAAGGT CTACAAAGGC
    ATGCTGGATA TTTTGAAGTG CACGGTGTCC AGTTTGGAGC ATTCATACAC TAATGCTGGC
    TTAGGGGGCA TGGCCAGTGT CTTCAGTCTG CTAGAGATAG CACGCACCCA CTACCAAACC
    AAAGATCCAG AGAAGCGGAA ACGGAGCCCC ACCGAAGGGC TTAGCAGTCC AGGCAGTAAG
    GAGAGCCCAT CGGGCCGAAT GGAGAGTGCC AGGGCGGCAG GGGTGCTTCT GGTACCCCGT
    ATCCAATTGC CGCCACCCTC CTCTGGGAAA TCATCACGCC AGTTTGATAC CCGGAGTCTG
    AACGAGGAGA ATTTTATTGC CTCCATTGGA GCGGATGGAG TAAAACAACG TGTAGAAGGA
    GGAGACACTG AGGAGAAGAA ATCACAGATC AGTGCAGACA GTGGCCTCAG TGTGACATCT
    GGCTCACAGA AGAGTGATAC GGATTCCCTG GCGAGCTCTG AACCTCCTGC TCTGACCAGA
    AGCACCAGCC AGGACTCAGA GGCCAGCACA GTGGTAAGTA ACAGCTCAGG TGAGACGCTC
    GGTGCAGACA GTGACCTGAG CAGCACTGCT GGAGATGGCC TGACAGGAAG ACATGGTCAG
    CATCTCAACC TGTCCCGTGG CACACTGTCT GACAGTGAGA TTGAGACCAA CCCTGCAACC
    AGCTCAGTGT TTGGCAAGAC ACACAAGCTG AAGCCAGGAG TGAAGGAGCC TGTTGGTGTT
    AATAAAGCAG CACCTGCTCC ACCCGTTGAG GATGTTAGCA TGAGGATCTA TCTGTGTGAG
    GGGCTTCTGG GTAAAGAGCG CTCAACTCTG TGGGATCAGA TGCAGTTCTG GGAGGACGCC
    TTCCTGGATG CCGTAATGCT GGAACGGGAA GGAATGGGAA TGGATCAGGG ACCACAAGAA
    ATGATTGACA GGTATCTTTC TCTGGGAGAT CATGACCGCA AGAGACTGGA GGATGATGAG
    GACAGACTGC TGGCCACGCT TCTGCACAAT ATGATTGCTT ACATGCTTAT GATGAAGGTC
    GGTAAAAACG ACATCAGGAA GAAAGTACGT CGTCTTATGG GCAAATCCCA CATTGGCCTC
    ACTCACAGTC AGGAGATCAA TGAAGTCCTG GATCGCCTCG CACATTTAAG CGGTCGGGAG
    CTTTCTATCA GGCCGAGTGG CAGCAGGCAT ATTAAAAAAC AGACATTTGT TGTGCATGCT
    GGGACAGATA CAACTGGTGA CATATTCTTC ATGGAGGTGT GTGATGATTG CATCGTCTTG
    CGCAGTAATA TTGGCACAGT CTATGAGCGC TGGTGGTATG AGAAGCTCAT AAATATGACT
    TACTGTCCCA AAACTAAAGT GTTGTGTCTG TGGAGGAGAA ACGGCCAGGA GACGCAGCTA
    AACAAGTTTT ACACTAAGAA GTGTCGGGAA TTGTACTACT GTGTTAAAGA CAGCATGGAG
    CGTGCTGCTG CTCGACAACA AAGCATCAAA CCAGGTCCAG AGCTTGGAGG CGAGTTTCCG
    GTTCAGGACA TGAAGACAGG GGAGGGTGGT CTCCTCCAAG TCACACTGGA AGGCATCAAC
    CTCAAATTTA TGCACAGCCA GGTTTTCATA GAGCTGAATC ACATTAAAAA GTGCAATACA
    GTGAAAGGAG TCTTTGTCCT GGAGGAGTTT GTTCCTGAAA CTAAAGAAGT GGTGATCCAT
    AAGTACAAGA CTCCTATGGC ACATCAGATC TGTTACTCGG TGCTCTGCCT CTTCTCCTAC
    ATGGCGGCGG TCAAGGGGAA AGAGTCAGAG GGCAAACCCA AGATGCTGTC CCCTCGTCCA
    CTAGCGAGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa143485
    Clone ID Related Accession (Same CDS sequence) XM_021477897.1
    Accession Version XM_021477897.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4881bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X23
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA TTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGAACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCAGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT ACCCAAGCTC TGCCCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTTCCCAGC CTCAAAGGTG CCCGAGCCCC CAGAGCACTG
    GTAGATCAGA AATCCTCTGT TATTAAGCAC AGCCCTACTG TGAAGAGAGA GTCTCCGTCT
    CCTCAAGGCA GAGCCAACAA TACCAGTGAG AACCAGCAGT TTCTAAAGGA GGTGGTCCAG
    AGCGTGCTGG AGGGGCAGGG TGTGGGCTGG CTTAACATGA AGAAGGTGCG TCGCCTGCTA
    GAGAATGAAC AGCTCAGAGT CTTTGTGCTC AGCAAACTCA ACCGTGCGGT CCAGTCAGAG
    GAGGACGCCC AGCAGGAAGT TATAAGAGAT GTGGAGATAA ATAGAAAGGT CTACAAAGGC
    ATGCTGGATA TTTTGAAGTG CACGGTGTCC AGTTTGGAGC ATTCATACAC TAATGCTGGC
    TTAGGGGGCA TGGCCAGTGT CTTCAGTCTG CTAGAGATAG CACGCACCCA CTACCAAACC
    AAAGATCCAG AGAAGCGGAA ACGGAGCCCC ACCGAAGGGC TTAGCAGTCC AGGCAGTAAG
    GAGAGCCCAT CGGGCCGAAT GGAGAGTGCC AGGGCGGCAG GGGTGCTTCT GGTACCCCGT
    ATCCAATTGC CGCCACCCTC CTCTGGGAAA TCATCACGCC AGTTTGATAC CCGGAGTCTG
    AACGAGGAGA ATTTTATTGC CTCCATTGGA GCGGATGGAG TAAAACAACG TGTAGAAGGA
    GGAGACACTG AGGAGAAGAA ATCACAGATC AGTGCAGACA GTGGCCTCAG TGTGACATCT
    GGCTCACAGA AGAGTGATAC GGATTCCCTG GCGAGCTCTG AACCTCCTGC TCTGACCAGA
    AGCACCAGCC AGGACTCAGA GGCCAGCACA GTGGTAAGTA ACAGCTCAGG TGAGACGCTC
    GGTGCAGACA GTGACCTGAG CAGCACTGCT GGAGATGGCC TGACAGGAAG ACATGGTCAG
    CATCTCAACC TGTCCCGTGG CACACTGTCT GACAGTGAGA TTGAGACCAA CCCTGCAACC
    AGCTCAGTGT TTGGCAAGAC ACACAAGCTG AAGCCAGGAG TGAAGGAGCC TGTTGGTGTT
    AATAAAGCAG CACCTGCTCC ACCCGTTGAG GATGTTAGCA TGAGGATCTA TCTGTGTGAG
    GGGCTTCTGG GTAAAGAGCG CTCAACTCTG TGGGATCAGA TGCAGTTCTG GGAGGACGCC
    TTCCTGGATG CCGTAATGCT GGAACGGGAA GGAATGGGAA TGGATCAGGG ACCACAAGAA
    ATGATTGACA GGTATCTTTC TCTGGGAGAT CATGACCGCA AGAGACTGGA GGATGATGAG
    GACAGACTGC TGGCCACGCT TCTGCACAAT ATGATTGCTT ACATGCTTAT GATGAAGGTC
    GGTAAAAACG ACATCAGGAA GAAAGTACGT CGTCTTATGG GCAAATCCCA CATTGGCCTC
    ACTCACAGTC AGGAGATCAA TGAAGTCCTG GATCGCCTCG CACATTTAAG CGGTCGGGAG
    CTTTCTATCA GGCCGAGTGG CAGCAGGCAT ATTAAAAAAC AGACATTTGT TGTGCATGCT
    GGGACAGATA CAACTGGTGA CATATTCTTC ATGGAGGTGT GTGATGATTG CATCGTCTTG
    CGCAGTAATA TTGGCACAGT CTATGAGCGC TGGTGGTATG AGAAGCTCAT AAATATGACT
    TACTGTCCCA AAACTAAAGT GTTGTGTCTG TGGAGGAGAA ACGGCCAGGA GACGCAGCTA
    AACAAGTTTT ACACTAAGAA GTGTCGGGAA TTGTACTACT GTGTTAAAGA CAGCATGGAG
    CGTGCTGCTG CTCGACAACA AAGCATCAAA CCAGGTCCAG AGCTTGGAGG CGAGTTTCCG
    GTTCAGGACA TGAAGACAGG GGAGGGTGGT CTCCTCCAAG TCACACTGGA AGGCATCAAC
    CTCAAATTTA TGCACAGCCA GGAGCGGAAG GTTTTCATAG AGCTGAATCA CATTAAAAAG
    TGCAATACAG TGAAAGGAGT CTTTGTCCTG GAGGAGTTTG TTCCTGAAAC TAAAGAAGTG
    GTGATCCATA AGTACAAGAC TCCTATGGCA CATCAGATCT GTTACTCGGT GCTCTGCCTC
    TTCTCCTACA TGGCGGCGGT CAAGGGGAAA GAGTCAGAGG GCAAACCCAA GATGCTGTCC
    CCTCGTCCAC TAGCGAGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333572.1
    CDS432..5312
    Translation

    Target ORF information:

    RefSeq Version XM_021477897.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X28, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021477897.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA TTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGAACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCAGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT ACCCAAGCTC TGCCCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTTCCCAGC CTCAAAGGTG CCCGAGCCCC CAGAGCACTG
    GTAGATCAGA AATCCTCTGT TATTAAGCAC AGCCCTACTG TGAAGAGAGA GTCTCCGTCT
    CCTCAAGGCA GAGCCAACAA TACCAGTGAG AACCAGCAGT TTCTAAAGGA GGTGGTCCAG
    AGCGTGCTGG AGGGGCAGGG TGTGGGCTGG CTTAACATGA AGAAGGTGCG TCGCCTGCTA
    GAGAATGAAC AGCTCAGAGT CTTTGTGCTC AGCAAACTCA ACCGTGCGGT CCAGTCAGAG
    GAGGACGCCC AGCAGGAAGT TATAAGAGAT GTGGAGATAA ATAGAAAGGT CTACAAAGGC
    ATGCTGGATA TTTTGAAGTG CACGGTGTCC AGTTTGGAGC ATTCATACAC TAATGCTGGC
    TTAGGGGGCA TGGCCAGTGT CTTCAGTCTG CTAGAGATAG CACGCACCCA CTACCAAACC
    AAAGATCCAG AGAAGCGGAA ACGGAGCCCC ACCGAAGGGC TTAGCAGTCC AGGCAGTAAG
    GAGAGCCCAT CGGGCCGAAT GGAGAGTGCC AGGGCGGCAG GGGTGCTTCT GGTACCCCGT
    ATCCAATTGC CGCCACCCTC CTCTGGGAAA TCATCACGCC AGTTTGATAC CCGGAGTCTG
    AACGAGGAGA ATTTTATTGC CTCCATTGGA GCGGATGGAG TAAAACAACG TGTAGAAGGA
    GGAGACACTG AGGAGAAGAA ATCACAGATC AGTGCAGACA GTGGCCTCAG TGTGACATCT
    GGCTCACAGA AGAGTGATAC GGATTCCCTG GCGAGCTCTG AACCTCCTGC TCTGACCAGA
    AGCACCAGCC AGGACTCAGA GGCCAGCACA GTGGTAAGTA ACAGCTCAGG TGAGACGCTC
    GGTGCAGACA GTGACCTGAG CAGCACTGCT GGAGATGGCC TGACAGGAAG ACATGGTCAG
    CATCTCAACC TGTCCCGTGG CACACTGTCT GACAGTGAGA TTGAGACCAA CCCTGCAACC
    AGCTCAGTGT TTGGCAAGAC ACACAAGCTG AAGCCAGGAG TGAAGGAGCC TGTTGGTGTT
    AATAAAGCAG CACCTGCTCC ACCCGTTGAG GATGTTAGCA TGAGGATCTA TCTGTGTGAG
    GGGCTTCTGG GTAAAGAGCG CTCAACTCTG TGGGATCAGA TGCAGTTCTG GGAGGACGCC
    TTCCTGGATG CCGTAATGCT GGAACGGGAA GGAATGGGAA TGGATCAGGG ACCACAAGAA
    ATGATTGACA GGTATCTTTC TCTGGGAGAT CATGACCGCA AGAGACTGGA GGATGATGAG
    GACAGACTGC TGGCCACGCT TCTGCACAAT ATGATTGCTT ACATGCTTAT GATGAAGGTC
    GGTAAAAACG ACATCAGGAA GAAAGTACGT CGTCTTATGG GCAAATCCCA CATTGGCCTC
    ACTCACAGTC AGGAGATCAA TGAAGTCCTG GATCGCCTCG CACATTTAAG CGGTCGGGAG
    CTTTCTATCA GGCCGAGTGG CAGCAGGCAT ATTAAAAAAC AGACATTTGT TGTGCATGCT
    GGGACAGATA CAACTGGTGA CATATTCTTC ATGGAGGTGT GTGATGATTG CATCGTCTTG
    CGCAGTAATA TTGGCACAGT CTATGAGCGC TGGTGGTATG AGAAGCTCAT AAATATGACT
    TACTGTCCCA AAACTAAAGT GTTGTGTCTG TGGAGGAGAA ACGGCCAGGA GACGCAGCTA
    AACAAGTTTT ACACTAAGAA GTGTCGGGAA TTGTACTACT GTGTTAAAGA CAGCATGGAG
    CGTGCTGCTG CTCGACAACA AAGCATCAAA CCAGGTCCAG AGCTTGGAGG CGAGTTTCCG
    GTTCAGGACA TGAAGACAGG GGAGGGTGGT CTCCTCCAAG TCACACTGGA AGGCATCAAC
    CTCAAATTTA TGCACAGCCA GGAGCGGAAG GTTTTCATAG AGCTGAATCA CATTAAAAAG
    TGCAATACAG TGAAAGGAGT CTTTGTCCTG GAGGAGTTTG TTCCTGAAAC TAAAGAAGTG
    GTGATCCATA AGTACAAGAC TCCTATGGCA CATCAGATCT GTTACTCGGT GCTCTGCCTC
    TTCTCCTACA TGGCGGCGGT CAAGGGGAAA GAGTCAGAGG GCAAACCCAA GATGCTGTCC
    CCTCGTCCAC TAGCGAGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa143486
    Clone ID Related Accession (Same CDS sequence) XM_021477899.1
    Accession Version XM_021477899.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4872bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X25
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA TTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGAACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCAGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT ACCCAAGCTC TGCCCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTTCCCAGC CTCAAAGGTG CCCGAGCCCC CAGAGCACTG
    GTAGATCAGA AATCCTCTGT TATTAAGCAC AGCCCTACTG TGAAGAGAGA GTCTCCGTCT
    CCTCAAGGCA GAGCCAACAA TACCAGTGAG AACCAGCAGT TTCTAAAGGA GGTGGTCCAG
    AGCGTGCTGG AGGGGCAGGG TGTGGGCTGG CTTAACATGA AGAAGGTGCG TCGCCTGCTA
    GAGAATGAAC AGCTCAGAGT CTTTGTGCTC AGCAAACTCA ACCGTGCGGT CCAGTCAGAG
    GAGGACGCCC AGCAGGAAGT TATAAGAGAT GTGGAGATAA ATAGAAAGGT CTACAAAGGC
    ATGCTGGATA TTTTGAAGTG CACGGTGTCC AGTTTGGAGC ATTCATACAC TAATGCTGGC
    TTAGGGGGCA TGGCCAGTGT CTTCAGTCTG CTAGAGATAG CACGCACCCA CTACCAAACC
    AAAGATCCAG AGAAGCGGAA ACGGAGCCCC ACCGAAGGGC TTAGCAGTCC AGGCAGTAAG
    GAGAGCCCAT CGGGCCGAAT GGAGAGTGCC AGGGCGGCAG GGGTGCTTCT GGTACCCCGT
    ATCCAATTGC CGCCACCCTC CTCTGGGAAA TCATCACGCC AGTTTGATAC CCGGAGTCTG
    AACGAGGAGA ATTTTATTGC CTCCATTGGA GCGGATGGAG TAAAACAACG TGTAGAAGGA
    GGAGACACTG AGGAGAAGAA ATCACAGATC AGTGCAGACA GTGGCCTCAG TGTGACATCT
    GGCTCACAGA AGAGTGATAC GGATTCCCTG GCGAGCTCTG AACCTCCTGC TCTGACCAGA
    AGCACCAGCC AGGACTCAGA GGCCAGCACA GTGGTAAGTA ACAGCTCAGG TGAGACGCTC
    GGTGCAGACA GTGACCTGAG CAGCACTGCT GGAGATGGCC TGACAGGAAG ACATGGTCAG
    CATCTCAACC TGTCCCGTGG CACACTGTCT GACAGTGAGA TTGAGACCAA CCCTGCAACC
    AGCTCAGTGT TTGGCAAGAC ACACAAGCTG AAGCCAGGAG TGAAGGAGCC TGTTGGTGTT
    AATAAAGCAG CACCTGCTCC ACCCGTTGAG GATGTTAGCA TGAGGATCTA TCTGTGTGAG
    GGGCTTCTGG GTAAAGAGCG CTCAACTCTG TGGGATCAGA TGCAGTTCTG GGAGGACGCC
    TTCCTGGATG CCGTAATGCT GGAACGGGAA GGAATGGGAA TGGATCAGGG ACCACAAGAA
    ATGATTGACA GGTATCTTTC TCTGGGAGAT CATGACCGCA AGAGACTGGA GGATGATGAG
    GACAGACTGC TGGCCACGCT TCTGCACAAT ATGATTGCTT ACATGCTTAT GATGAAGGTC
    GGTAAAAACG ACATCAGGAA GAAAGTACGT CGTCTTATGG GCAAATCCCA CATTGGCCTC
    ACTCACAGTC AGGAGATCAA TGAAGTCCTG GATCGCCTCG CACATTTAAG CGGTCGGGAG
    CTTTCTATCA GGCCGAGTGG CAGCAGGCAT ATTAAAAAAC AGACATTTGT TGTGCATGCT
    GGGACAGATA CAACTGGTGA CATATTCTTC ATGGAGGTGT GTGATGATTG CATCGTCTTG
    CGCAGTAATA TTGGCACAGT CTATGAGCGC TGGTGGTATG AGAAGCTCAT AAATATGACT
    TACTGTCCCA AAACTAAAGT GTTGTGTCTG TGGAGGAGAA ACGGCCAGGA GACGCAGCTA
    AACAAGTTTT ACACTAAGAA GTGTCGGGAA TTGTACTACT GTGTTAAAGA CAGCATGGAG
    CGTGCTGCTG CTCGACAACA AAGCATCAAA CCAGGTCCAG AGCTTGGAGG CGAGTTTCCG
    GTTCAGGACA TGAAGACAGG GGAGGGTGGT CTCCTCCAAG TCACACTGGA AGGCATCAAC
    CTCAAATTTA TGCACAGCCA GGTTTTCATA GAGCTGAATC ACATTAAAAA GTGCAATACA
    GTGAAAGGAG TCTTTGTCCT GGAGGAGTTT GTTCCTGAAA CTAAAGAAGT GGTGATCCAT
    AAGTACAAGA CTCCTATGGC ACATCAGATC TGTTACTCGG TGCTCTGCCT CTTCTCCTAC
    ATGGCGGCGG TCAAGGGGAA AGAGTCAGAG GGCAAACCCA AGATGCTGTC CCCTCGTCCA
    CTAGCGAGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333574.1
    CDS432..5303
    Translation

    Target ORF information:

    RefSeq Version XM_021477899.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X29, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021477899.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA TTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGAACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCAGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT ACCCAAGCTC TGCCCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTTCCCAGC CTCAAAGGTG CCCGAGCCCC CAGAGCACTG
    GTAGATCAGA AATCCTCTGT TATTAAGCAC AGCCCTACTG TGAAGAGAGA GTCTCCGTCT
    CCTCAAGGCA GAGCCAACAA TACCAGTGAG AACCAGCAGT TTCTAAAGGA GGTGGTCCAG
    AGCGTGCTGG AGGGGCAGGG TGTGGGCTGG CTTAACATGA AGAAGGTGCG TCGCCTGCTA
    GAGAATGAAC AGCTCAGAGT CTTTGTGCTC AGCAAACTCA ACCGTGCGGT CCAGTCAGAG
    GAGGACGCCC AGCAGGAAGT TATAAGAGAT GTGGAGATAA ATAGAAAGGT CTACAAAGGC
    ATGCTGGATA TTTTGAAGTG CACGGTGTCC AGTTTGGAGC ATTCATACAC TAATGCTGGC
    TTAGGGGGCA TGGCCAGTGT CTTCAGTCTG CTAGAGATAG CACGCACCCA CTACCAAACC
    AAAGATCCAG AGAAGCGGAA ACGGAGCCCC ACCGAAGGGC TTAGCAGTCC AGGCAGTAAG
    GAGAGCCCAT CGGGCCGAAT GGAGAGTGCC AGGGCGGCAG GGGTGCTTCT GGTACCCCGT
    ATCCAATTGC CGCCACCCTC CTCTGGGAAA TCATCACGCC AGTTTGATAC CCGGAGTCTG
    AACGAGGAGA ATTTTATTGC CTCCATTGGA GCGGATGGAG TAAAACAACG TGTAGAAGGA
    GGAGACACTG AGGAGAAGAA ATCACAGATC AGTGCAGACA GTGGCCTCAG TGTGACATCT
    GGCTCACAGA AGAGTGATAC GGATTCCCTG GCGAGCTCTG AACCTCCTGC TCTGACCAGA
    AGCACCAGCC AGGACTCAGA GGCCAGCACA GTGGTAAGTA ACAGCTCAGG TGAGACGCTC
    GGTGCAGACA GTGACCTGAG CAGCACTGCT GGAGATGGCC TGACAGGAAG ACATGGTCAG
    CATCTCAACC TGTCCCGTGG CACACTGTCT GACAGTGAGA TTGAGACCAA CCCTGCAACC
    AGCTCAGTGT TTGGCAAGAC ACACAAGCTG AAGCCAGGAG TGAAGGAGCC TGTTGGTGTT
    AATAAAGCAG CACCTGCTCC ACCCGTTGAG GATGTTAGCA TGAGGATCTA TCTGTGTGAG
    GGGCTTCTGG GTAAAGAGCG CTCAACTCTG TGGGATCAGA TGCAGTTCTG GGAGGACGCC
    TTCCTGGATG CCGTAATGCT GGAACGGGAA GGAATGGGAA TGGATCAGGG ACCACAAGAA
    ATGATTGACA GGTATCTTTC TCTGGGAGAT CATGACCGCA AGAGACTGGA GGATGATGAG
    GACAGACTGC TGGCCACGCT TCTGCACAAT ATGATTGCTT ACATGCTTAT GATGAAGGTC
    GGTAAAAACG ACATCAGGAA GAAAGTACGT CGTCTTATGG GCAAATCCCA CATTGGCCTC
    ACTCACAGTC AGGAGATCAA TGAAGTCCTG GATCGCCTCG CACATTTAAG CGGTCGGGAG
    CTTTCTATCA GGCCGAGTGG CAGCAGGCAT ATTAAAAAAC AGACATTTGT TGTGCATGCT
    GGGACAGATA CAACTGGTGA CATATTCTTC ATGGAGGTGT GTGATGATTG CATCGTCTTG
    CGCAGTAATA TTGGCACAGT CTATGAGCGC TGGTGGTATG AGAAGCTCAT AAATATGACT
    TACTGTCCCA AAACTAAAGT GTTGTGTCTG TGGAGGAGAA ACGGCCAGGA GACGCAGCTA
    AACAAGTTTT ACACTAAGAA GTGTCGGGAA TTGTACTACT GTGTTAAAGA CAGCATGGAG
    CGTGCTGCTG CTCGACAACA AAGCATCAAA CCAGGTCCAG AGCTTGGAGG CGAGTTTCCG
    GTTCAGGACA TGAAGACAGG GGAGGGTGGT CTCCTCCAAG TCACACTGGA AGGCATCAAC
    CTCAAATTTA TGCACAGCCA GGTTTTCATA GAGCTGAATC ACATTAAAAA GTGCAATACA
    GTGAAAGGAG TCTTTGTCCT GGAGGAGTTT GTTCCTGAAA CTAAAGAAGT GGTGATCCAT
    AAGTACAAGA CTCCTATGGC ACATCAGATC TGTTACTCGG TGCTCTGCCT CTTCTCCTAC
    ATGGCGGCGG TCAAGGGGAA AGAGTCAGAG GGCAAACCCA AGATGCTGTC CCCTCGTCCA
    CTAGCGAGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa143487
    Clone ID Related Accession (Same CDS sequence) XM_021477900.1
    Accession Version XM_021477900.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4869bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X26
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA TTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGAACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCAGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT ACCCAAGCTC TGCCCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTTCCCAGC CTCAAAGAAT ATTTTAATTT TGATTTAGCA
    GAGGACACAG ATCAAGACGA TGCTGATGAC AGTCCTGAAA GTCCTGTATT TGGGCTAAAT
    TCTCTAATGG AGATTGTAAC AGAGATCGGC CCGGGGAGCG GAGAAGGTGC CCGAGCCCCC
    AGAGCACTGG TAGATCAGAA ATCCTCTGTT ATTAAGCACA GCCCTACTGT GAAGAGAGAG
    TCTCCGTCTC CTCAAGGCAG AGCCAACAAT ACCAGTGAGA ACCAGCAGTT TCTAAAGGAG
    GTGGTCCAGA GCGTGCTGGA GGGGCAGGGT GTGGGCTGGC TTAACATGAA GAAGGTGCGT
    CGCCTGCTAG AGAATGAACA GCTCAGAGTC TTTGTGCTCA GCAAACTCAA CCGTGCGGTC
    CAGTCAGAGG AGGACGCCCA GCAGGAAGTT ATAAGAGATG TGGAGATAAA TAGAAAGGTC
    TACAAAGGCA TGCTGGATAT TTTGAAGTGC ACGGTGTCCA GTTTGGAGCA TTCATACACT
    AATGCTGGCT TAGGGGGCAT GGCCAGTGTC TTCAGTCTGC TAGAGATAGC ACGCACCCAC
    TACCAAACCA AAGGAGCGGA TGGAGTAAAA CAACGTGTAG AAGGAGGAGA CACTGAGGAG
    AAGAAATCAC AGATCAGTGC AGACAGTGGC CTCAGTGTGA CATCTGGCTC ACAGAAGAGT
    GATACGGATT CCCTGGCGAG CTCTGAACCT CCTGCTCTGA CCAGAAGCAC CAGCCAGGAC
    TCAGAGGCCA GCACAGTGGT AAGTAACAGC TCAGGTGAGA CGCTCGGTGC AGACAGTGAC
    CTGAGCAGCA CTGCTGGAGA TGGCCTGACA GGAAGACATG GTCAGCATCT CAACCTGTCC
    CGTGGCACAC TGTCTGACAG TGAGATTGAG ACCAACCCTG CAACCAGCTC AGTGTTTGGC
    AAGACACACA AGCTGAAGCC AGGAGTGAAG GAGCCTGTTG GTGTTAATAA AGCAGCACCT
    GCTCCACCCG TTGAGGATGT TAGCATGAGG ATCTATCTGT GTGAGGGGCT TCTGGGCCGG
    GATAAAAGCT CAGTTTGGGA CCAGTTGGAG GATGCTGCAA TGGAAACCTT TTCTCTGAGT
    AAAGAGCGCT CAACTCTGTG GGATCAGATG CAGTTCTGGG AGGACGCCTT CCTGGATGCC
    GTAATGCTGG AACGGGAAGG AATGGGAATG GATCAGGGAC CACAAGAAAT GATTGACAGG
    TATCTTTCTC TGGGAGATCA TGACCGCAAG AGACTGGAGG ATGATGAGGA CAGACTGCTG
    GCCACGCTTC TGCACAATAT GATTGCTTAC ATGCTTATGA TGAAGGTCGG TAAAAACGAC
    ATCAGGAAGA AAGTACGTCG TCTTATGGGC AAATCCCACA TTGGCCTCAC TCACAGTCAG
    GAGATCAATG AAGTCCTGGA TCGCCTCGCA CATTTAAGCG GTCGGGAGCT TTCTATCAGG
    CCGAGTGGCA GCAGGCATAT TAAAAAACAG ACATTTGTTG TGCATGCTGG GACAGATACA
    ACTGGTGACA TATTCTTCAT GGAGGTGTGT GATGATTGCA TCGTCTTGCG CAGTAATATT
    GGCACAGTCT ATGAGCGCTG GTGGTATGAG AAGCTCATAA ATATGACTTA CTGTCCCAAA
    ACTAAAGTGT TGTGTCTGTG GAGGAGAAAC GGCCAGGAGA CGCAGCTAAA CAAGTTTTAC
    ACTAAGAAGT GTCGGGAATT GTACTACTGT GTTAAAGACA GCATGGAGCG TGCTGCTGCT
    CGACAACAAA GCATCAAACC AGGTCCAGAG CTTGGAGGCG AGTTTCCGGT TCAGGACATG
    AAGACAGGGG AGGGTGGTCT CCTCCAAGTC ACACTGGAAG GCATCAACCT CAAATTTATG
    CACAGCCAGG AGCGGAAGGT TTTCATAGAG CTGAATCACA TTAAAAAGTG CAATACAGTG
    AAAGGAGTCT TTGTCCTGGA GGAGTTTGTT CCTGAAACTA AAGAAGTGGT GATCCATAAG
    TACAAGACTC CTATGGCACA TCAGATCTGT TACTCGGTGC TCTGCCTCTT CTCCTACATG
    GCGGCGGTCA AGGGGAAAGA GTCAGAGGGC AAACCCAAGA TGCTGTCCCC TCGTCCACTA
    GCGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333575.1
    CDS432..5300
    Translation

    Target ORF information:

    RefSeq Version XM_021477900.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X30, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021477900.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA TTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGAACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCAGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT ACCCAAGCTC TGCCCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTTCCCAGC CTCAAAGAAT ATTTTAATTT TGATTTAGCA
    GAGGACACAG ATCAAGACGA TGCTGATGAC AGTCCTGAAA GTCCTGTATT TGGGCTAAAT
    TCTCTAATGG AGATTGTAAC AGAGATCGGC CCGGGGAGCG GAGAAGGTGC CCGAGCCCCC
    AGAGCACTGG TAGATCAGAA ATCCTCTGTT ATTAAGCACA GCCCTACTGT GAAGAGAGAG
    TCTCCGTCTC CTCAAGGCAG AGCCAACAAT ACCAGTGAGA ACCAGCAGTT TCTAAAGGAG
    GTGGTCCAGA GCGTGCTGGA GGGGCAGGGT GTGGGCTGGC TTAACATGAA GAAGGTGCGT
    CGCCTGCTAG AGAATGAACA GCTCAGAGTC TTTGTGCTCA GCAAACTCAA CCGTGCGGTC
    CAGTCAGAGG AGGACGCCCA GCAGGAAGTT ATAAGAGATG TGGAGATAAA TAGAAAGGTC
    TACAAAGGCA TGCTGGATAT TTTGAAGTGC ACGGTGTCCA GTTTGGAGCA TTCATACACT
    AATGCTGGCT TAGGGGGCAT GGCCAGTGTC TTCAGTCTGC TAGAGATAGC ACGCACCCAC
    TACCAAACCA AAGGAGCGGA TGGAGTAAAA CAACGTGTAG AAGGAGGAGA CACTGAGGAG
    AAGAAATCAC AGATCAGTGC AGACAGTGGC CTCAGTGTGA CATCTGGCTC ACAGAAGAGT
    GATACGGATT CCCTGGCGAG CTCTGAACCT CCTGCTCTGA CCAGAAGCAC CAGCCAGGAC
    TCAGAGGCCA GCACAGTGGT AAGTAACAGC TCAGGTGAGA CGCTCGGTGC AGACAGTGAC
    CTGAGCAGCA CTGCTGGAGA TGGCCTGACA GGAAGACATG GTCAGCATCT CAACCTGTCC
    CGTGGCACAC TGTCTGACAG TGAGATTGAG ACCAACCCTG CAACCAGCTC AGTGTTTGGC
    AAGACACACA AGCTGAAGCC AGGAGTGAAG GAGCCTGTTG GTGTTAATAA AGCAGCACCT
    GCTCCACCCG TTGAGGATGT TAGCATGAGG ATCTATCTGT GTGAGGGGCT TCTGGGCCGG
    GATAAAAGCT CAGTTTGGGA CCAGTTGGAG GATGCTGCAA TGGAAACCTT TTCTCTGAGT
    AAAGAGCGCT CAACTCTGTG GGATCAGATG CAGTTCTGGG AGGACGCCTT CCTGGATGCC
    GTAATGCTGG AACGGGAAGG AATGGGAATG GATCAGGGAC CACAAGAAAT GATTGACAGG
    TATCTTTCTC TGGGAGATCA TGACCGCAAG AGACTGGAGG ATGATGAGGA CAGACTGCTG
    GCCACGCTTC TGCACAATAT GATTGCTTAC ATGCTTATGA TGAAGGTCGG TAAAAACGAC
    ATCAGGAAGA AAGTACGTCG TCTTATGGGC AAATCCCACA TTGGCCTCAC TCACAGTCAG
    GAGATCAATG AAGTCCTGGA TCGCCTCGCA CATTTAAGCG GTCGGGAGCT TTCTATCAGG
    CCGAGTGGCA GCAGGCATAT TAAAAAACAG ACATTTGTTG TGCATGCTGG GACAGATACA
    ACTGGTGACA TATTCTTCAT GGAGGTGTGT GATGATTGCA TCGTCTTGCG CAGTAATATT
    GGCACAGTCT ATGAGCGCTG GTGGTATGAG AAGCTCATAA ATATGACTTA CTGTCCCAAA
    ACTAAAGTGT TGTGTCTGTG GAGGAGAAAC GGCCAGGAGA CGCAGCTAAA CAAGTTTTAC
    ACTAAGAAGT GTCGGGAATT GTACTACTGT GTTAAAGACA GCATGGAGCG TGCTGCTGCT
    CGACAACAAA GCATCAAACC AGGTCCAGAG CTTGGAGGCG AGTTTCCGGT TCAGGACATG
    AAGACAGGGG AGGGTGGTCT CCTCCAAGTC ACACTGGAAG GCATCAACCT CAAATTTATG
    CACAGCCAGG AGCGGAAGGT TTTCATAGAG CTGAATCACA TTAAAAAGTG CAATACAGTG
    AAAGGAGTCT TTGTCCTGGA GGAGTTTGTT CCTGAAACTA AAGAAGTGGT GATCCATAAG
    TACAAGACTC CTATGGCACA TCAGATCTGT TACTCGGTGC TCTGCCTCTT CTCCTACATG
    GCGGCGGTCA AGGGGAAAGA GTCAGAGGGC AAACCCAAGA TGCTGTCCCC TCGTCCACTA
    GCGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa143489
    Clone ID Related Accession (Same CDS sequence) XM_021477906.1
    Accession Version XM_021477906.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4665bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X32
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA TTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGAACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCAGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT ACCCAAGCTC TGCCCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTTCCCAGC CTCAAAGGTG CCCGAGCCCC CAGAGCACTG
    GTAGATCAGA AATCCTCTGT TATTAAGCAC AGCCCTACTG TGAAGAGAGA GTCTCCGTCT
    CCTCAAGGCA GAGCCAACAA TACCAGTGAG AACCAGCAGT TTCTAAAGGA GGTGGTCCAG
    AGCGTGCTGG AGGGGCAGGG TGTGGGCTGG CTTAACATGA AGAAGGTGCG TCGCCTGCTA
    GAGAATGAAC AGCTCAGAGT CTTTGTGCTC AGCAAACTCA ACCGTGCGGT CCAGTCAGAG
    GAGGACGCCC AGCAGGAAGT TATAAGAGAT GTGGAGATAA ATAGAAAGGT CTACAAAGGC
    ATGCTGGATA TTTTGAAGTG CACGGTGTCC AGTTTGGAGC ATTCATACAC TAATGCTGGC
    TTAGGGGGCA TGGCCAGTGT CTTCAGTCTG CTAGAGATAG CACGCACCCA CTACCAAACC
    AAAGATCCAG AGAAGCGGAA ACGGAGCCCC ACCGAAGGGC TTAGCAGTCC AGGCAGTAAG
    GAGAGCCCAT CGGGCCGAAT GGAGAGTGCC AGGGCGGCAG GGGTGCTTCT GGTACCCCGT
    ATCCAATTGC CGCCACCCTC CTCTGGGAAA TCATCACGCC AGTTTGATAC CCGGAGTCTG
    AACGAGGAGA ATTTTATTGC CTCCATTGGA GCGGATGGAG TAAAACAACG TGTAGAAGGA
    GGAGACACTG AGGAGAAGAA ATCACAGATC AGTGCAGACA GTGGCCTCAG TGTGACATCT
    GGCTCACAGA AGAGTGATAC GGATTCCCTG GCGAGCTCTG AACCTCCTGC TCTGACCAGA
    AGCACCAGCC AGGACTCAGA GGCCAGCACA GTGGTAAGTA ACAGCTCAGG TGAGACGCTC
    GGTGCAGACA GTGACCTGAG CAGCACTGCT GGAGATGGCC TGACAGGAAG ACATGGTCAG
    CATCTCAACC TGTCCCGTGG CACACTGTCT GACAGTGAGA TTGAGACCAA CCCTGCAACC
    AGCTCAGTGT TTGGCAAGAC ACACAAGCTG AAGCCAGGAG TGAAGGAGCC TGTTGGTGTT
    AATAAAGCAG CACCTGCTCC ACCCGTTGAG GATGTTAGCA TGAGGATCTA TCTGTGTGAG
    GGGCTTCTGG GTAAAGAGCG CTCAACTCTG TGGGATCAGA TGCAGTTCTG GGAGGACGCC
    TTCCTGGATG CCGTAATGCT GGAACGGGAA GGAATGGGAA TGGATCAGGG ACCACAAGAA
    ATGATTGACA GGTATCTTTC TCTGGGAGAT CATGACCGCA AGAGACTGGA GGATGATGAG
    GACAGACTGC TGGCCACGCT TCTGCACAAT ATGATTGCTT ACATGCTTAT GATGAAGGTC
    GGTAAAAACG ACATCAGGAA GAAAGTACGT CGTCTTATGG GCAAATCCCA CATTGGCCTC
    ACTCACAGTC AGGAGATCAA TGAAGTCCTG GATCGCCTCG CACATTTAAG CGGTCGGGAG
    CTTTCTATCA GGCCGAGTGG CAGCAGGCAT ATTAAAAAAC AGACATTTGT TGTGCATGCT
    GGGACAGATA CAACTGGTGA CATATTCTTC ATGGAGGTGT GTGATGATTG CATCGTCTTG
    CGCAGTAATA TTGGCACAGT CTATGAGCGC TGGTGGTATG AGAAGCTCAT AAATATGACT
    TACTGTCCCA AAACTAAAGT GTTGTGTCTG TGGAGGAGAA ACGGCCAGGA GACGCAGCTA
    AACAAGTTTT ACACTAAGAA GTGTCGGGAA TTGTACTACT GTGTTAAAGA CAGCATGGAG
    CGTGCTGCTG CTCGACAACA AAGCATCAAA CCAGGTCCAG AGCTTGGAGG CGAGTTTCCG
    GTTCAGGACA TGAAGACAGG GGAGGGTGGT CTCCTCCAAG TCACACTGGA AGGCATCAAC
    CTCAAATTTA TGCACAGCCA GTTCCTGAAA CTAAAGAAGT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333581.1
    CDS432..5096
    Translation

    Target ORF information:

    RefSeq Version XM_021477906.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X32, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021477906.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA TTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGAACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCAGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT ACCCAAGCTC TGCCCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTTCCCAGC CTCAAAGGTG CCCGAGCCCC CAGAGCACTG
    GTAGATCAGA AATCCTCTGT TATTAAGCAC AGCCCTACTG TGAAGAGAGA GTCTCCGTCT
    CCTCAAGGCA GAGCCAACAA TACCAGTGAG AACCAGCAGT TTCTAAAGGA GGTGGTCCAG
    AGCGTGCTGG AGGGGCAGGG TGTGGGCTGG CTTAACATGA AGAAGGTGCG TCGCCTGCTA
    GAGAATGAAC AGCTCAGAGT CTTTGTGCTC AGCAAACTCA ACCGTGCGGT CCAGTCAGAG
    GAGGACGCCC AGCAGGAAGT TATAAGAGAT GTGGAGATAA ATAGAAAGGT CTACAAAGGC
    ATGCTGGATA TTTTGAAGTG CACGGTGTCC AGTTTGGAGC ATTCATACAC TAATGCTGGC
    TTAGGGGGCA TGGCCAGTGT CTTCAGTCTG CTAGAGATAG CACGCACCCA CTACCAAACC
    AAAGATCCAG AGAAGCGGAA ACGGAGCCCC ACCGAAGGGC TTAGCAGTCC AGGCAGTAAG
    GAGAGCCCAT CGGGCCGAAT GGAGAGTGCC AGGGCGGCAG GGGTGCTTCT GGTACCCCGT
    ATCCAATTGC CGCCACCCTC CTCTGGGAAA TCATCACGCC AGTTTGATAC CCGGAGTCTG
    AACGAGGAGA ATTTTATTGC CTCCATTGGA GCGGATGGAG TAAAACAACG TGTAGAAGGA
    GGAGACACTG AGGAGAAGAA ATCACAGATC AGTGCAGACA GTGGCCTCAG TGTGACATCT
    GGCTCACAGA AGAGTGATAC GGATTCCCTG GCGAGCTCTG AACCTCCTGC TCTGACCAGA
    AGCACCAGCC AGGACTCAGA GGCCAGCACA GTGGTAAGTA ACAGCTCAGG TGAGACGCTC
    GGTGCAGACA GTGACCTGAG CAGCACTGCT GGAGATGGCC TGACAGGAAG ACATGGTCAG
    CATCTCAACC TGTCCCGTGG CACACTGTCT GACAGTGAGA TTGAGACCAA CCCTGCAACC
    AGCTCAGTGT TTGGCAAGAC ACACAAGCTG AAGCCAGGAG TGAAGGAGCC TGTTGGTGTT
    AATAAAGCAG CACCTGCTCC ACCCGTTGAG GATGTTAGCA TGAGGATCTA TCTGTGTGAG
    GGGCTTCTGG GTAAAGAGCG CTCAACTCTG TGGGATCAGA TGCAGTTCTG GGAGGACGCC
    TTCCTGGATG CCGTAATGCT GGAACGGGAA GGAATGGGAA TGGATCAGGG ACCACAAGAA
    ATGATTGACA GGTATCTTTC TCTGGGAGAT CATGACCGCA AGAGACTGGA GGATGATGAG
    GACAGACTGC TGGCCACGCT TCTGCACAAT ATGATTGCTT ACATGCTTAT GATGAAGGTC
    GGTAAAAACG ACATCAGGAA GAAAGTACGT CGTCTTATGG GCAAATCCCA CATTGGCCTC
    ACTCACAGTC AGGAGATCAA TGAAGTCCTG GATCGCCTCG CACATTTAAG CGGTCGGGAG
    CTTTCTATCA GGCCGAGTGG CAGCAGGCAT ATTAAAAAAC AGACATTTGT TGTGCATGCT
    GGGACAGATA CAACTGGTGA CATATTCTTC ATGGAGGTGT GTGATGATTG CATCGTCTTG
    CGCAGTAATA TTGGCACAGT CTATGAGCGC TGGTGGTATG AGAAGCTCAT AAATATGACT
    TACTGTCCCA AAACTAAAGT GTTGTGTCTG TGGAGGAGAA ACGGCCAGGA GACGCAGCTA
    AACAAGTTTT ACACTAAGAA GTGTCGGGAA TTGTACTACT GTGTTAAAGA CAGCATGGAG
    CGTGCTGCTG CTCGACAACA AAGCATCAAA CCAGGTCCAG AGCTTGGAGG CGAGTTTCCG
    GTTCAGGACA TGAAGACAGG GGAGGGTGGT CTCCTCCAAG TCACACTGGA AGGCATCAAC
    CTCAAATTTA TGCACAGCCA GTTCCTGAAA CTAAAGAAGT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144881
    Clone ID Related Accession (Same CDS sequence) XM_021471950.1
    Accession Version XM_021471950.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5154bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394698.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA CTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGGACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCGGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT TCCCAAGCTC TGCTCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTCCCCAGC CTCAAAGAAT ATTTTAATTT TGATTTAGCA
    GAGGACACAG ATCAAGACGA TGCTGATGAC AGTCCTGAAA GTCCTGTATT TGGGCTAAAT
    TCTCTAATGG AGATTGTAAC AGAGATCGGC CCGGGGAGCG GAGAAGGTGC CCGAGCCCCC
    AGAGCACTGG TAGATCAGAA ATCCTCTGTT ATTAAGCACA GCCCTACTGT GAAGAGAGAG
    TCTCCGTCTC CTCAAGGCAG AGCCAACAAT ACCAGTGAGA ACCAGCAGTT TCTAAAGGAG
    GTGGTCCAGA GCGTGCTGGA GGGGCAGGGT GTGGGCTGGC TTAACATGAA GAAGGTGCGT
    CGCCTGCTAG AGAATGAACA GCTCAGAGTC TTTGTGCTCA GCAAACTCAA CCGTGCGGTC
    CAGTCAGAGG AGGACGCCCA GCAGGAAGTT ATAAGAGATG TGGAGATAAA TAGAAAGGTC
    TACAAAGGCA TGCTGGATAT TTTGAAGTGC ACGGTGTCCA GTTTGGAGCA TTCATACACT
    AATGCTGGCT TAGGGGGCAT GGCCAGTGTC TTCAGTCTGC TAGAGATAGC ACGCACCCAC
    TACCAAACCA AAGATCCAGA GAAGCGGAAA CGGAGCCCCA CCGAAGGGCT TAGCAGTCCA
    GGCAGTAAGG AGAGCCCATC GGGCCGAATG GAGAGTGCCA GGGCGGCAGG GGTGCTTCTG
    GTACCCCGTA TCCAATTGCC GCCACCCTCC TCTGGGAAAT CATCACGCCA GTTTGATACC
    CGGAGTCTGA ACGAGGAGAA TTTTATTGCC TCCATTGAAT TGTGGAGCAA GCACCAGGAT
    AACAGAAAGC AAAAAGCTTT GGAAAAAGAA CAGAGTGGTT TAAATAAACG GAAATCAGGA
    GCGGATGGAG TAAAACAACG TGTAGAAGGA GGAGACACTG AGGAGAAGAA ATCACAGATC
    AGTGCAGACA GTGGCCTCAG TGTGACATCT GGCTCACAGA AGAGTGATAC GGATTCCCTG
    GCGAGCTCTG AACCTCCTGC TCTGACCAGA AGCACCAGCC AGGACTCAGA GGCCAGCACA
    GTGGTAAGTA ACAGCTCAGG TGAGACGCTC GGTGCAGACA GTGACCTGAG CAGCACTGCT
    GGAGATGGCC TGACAGGAAG ACATGGTCAG CATCTCAACC TGTCCCGTGG CACACTGTCT
    GACAGTGAGA TTGAGACCAA CCCTGCAACC AGCTCAGTGT TTGGCAAGAC ACACAAGCTG
    AAGCCAGGAG TGAAGGAGCC TGTTGGTGTT AATAAAGCAG CACCTGCTCC ACCCGTTGAG
    GATGTTAGCA TGAGGATCTA TCTGTGTGAG GGGCTTCTGG GCCGGGATAA AAGCTCAGTT
    TGGGACCAGT TGGAGGATGC TGCAATGGAA ACCTTTTCTC TGAGTAAAGA GCGCTCAACT
    CTGTGGGATC AGATGCAGTT CTGGGAGGAC GCCTTCCTGG ATGCCGTAAT GCTGGAACGG
    GAAGGAATGG GAATGGATCA GGGACCACAA GAAATGATTG ACAGGTATCT TTCTCTGGGA
    GATCATGACC GCAAGAGACT GGAGGATGAT GAGGACAGAC TGCTGGCCAC GCTTCTGCAC
    AATATGATTG CTTACATGCT TATGATGAAG GTCGGTAAAA ACGACATCAG GAAGAAAGTA
    CGTCGTCTTA TGGGCAAATC CCACATTGGC CTCACTCACA GTCAGGAGAT CAATGAAGTC
    CTGGATCGCC TCGCACATTT AAGCGGTCGG GAGCTTTCTA TCAGGCCGAG TGGCAGCAGG
    CATATTAAAA AACAGACATT TGTTGTGCAT GCTGGGACAG ATACAACTGG TGACATATTC
    TTCATGGAGG TGTGTGATGA TTGCATCGTC TTGCGCAGTA ATATTGGCAC AGTCTATGAG
    CGCTGGTGGT ATGAGAAGCT CATAAATATG ACTTACTGTC CCAAAACTAA AGTGTTGTGT
    CTGTGGAGGA GAAACGGCCA GGAGACGCAG CTAAACAAGT TTTACACTAA GAAGTGTCGG
    GAATTGTACT ACTGTGTTAA AGACAGCATG GAGCGTGCTG CTGCTCGACA ACAAAGCATC
    AAACCAGGTC CAGAGCTTGG AGGCGAGTTT CCGGTTCAGG ACATGAAGAC AGGGGAGGGT
    GGTCTCCTCC AAGTCACACT GGAAGGCATC AACCTCAAAT TTATGCACAG CCAGGAGCGG
    AAGGTTTTCA TAGAGCTGAA TCACATTAAA AAGTGCAATA CAGTGAAAGG AGTCTTTGTC
    CTGGAGGAGT TTGTTCCTGA AACTAAAGAA GTGGTGATCC ATAAGTACAA GACTCCTATG
    GCACATCAGA TCTGTTACTC GGTGCTCTGC CTCTTCTCCT ACATGGCGGC GGTCAAGGGG
    AAAGAGTCAG AGGGCAAACC CAAGATGCTG TCCCCTCGTC CACTAGCGAG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327625.1
    CDS169..5322
    Translation

    Target ORF information:

    RefSeq Version XM_021471950.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X40, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471950.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA CTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGGACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCGGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT TCCCAAGCTC TGCTCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTCCCCAGC CTCAAAGAAT ATTTTAATTT TGATTTAGCA
    GAGGACACAG ATCAAGACGA TGCTGATGAC AGTCCTGAAA GTCCTGTATT TGGGCTAAAT
    TCTCTAATGG AGATTGTAAC AGAGATCGGC CCGGGGAGCG GAGAAGGTGC CCGAGCCCCC
    AGAGCACTGG TAGATCAGAA ATCCTCTGTT ATTAAGCACA GCCCTACTGT GAAGAGAGAG
    TCTCCGTCTC CTCAAGGCAG AGCCAACAAT ACCAGTGAGA ACCAGCAGTT TCTAAAGGAG
    GTGGTCCAGA GCGTGCTGGA GGGGCAGGGT GTGGGCTGGC TTAACATGAA GAAGGTGCGT
    CGCCTGCTAG AGAATGAACA GCTCAGAGTC TTTGTGCTCA GCAAACTCAA CCGTGCGGTC
    CAGTCAGAGG AGGACGCCCA GCAGGAAGTT ATAAGAGATG TGGAGATAAA TAGAAAGGTC
    TACAAAGGCA TGCTGGATAT TTTGAAGTGC ACGGTGTCCA GTTTGGAGCA TTCATACACT
    AATGCTGGCT TAGGGGGCAT GGCCAGTGTC TTCAGTCTGC TAGAGATAGC ACGCACCCAC
    TACCAAACCA AAGATCCAGA GAAGCGGAAA CGGAGCCCCA CCGAAGGGCT TAGCAGTCCA
    GGCAGTAAGG AGAGCCCATC GGGCCGAATG GAGAGTGCCA GGGCGGCAGG GGTGCTTCTG
    GTACCCCGTA TCCAATTGCC GCCACCCTCC TCTGGGAAAT CATCACGCCA GTTTGATACC
    CGGAGTCTGA ACGAGGAGAA TTTTATTGCC TCCATTGAAT TGTGGAGCAA GCACCAGGAT
    AACAGAAAGC AAAAAGCTTT GGAAAAAGAA CAGAGTGGTT TAAATAAACG GAAATCAGGA
    GCGGATGGAG TAAAACAACG TGTAGAAGGA GGAGACACTG AGGAGAAGAA ATCACAGATC
    AGTGCAGACA GTGGCCTCAG TGTGACATCT GGCTCACAGA AGAGTGATAC GGATTCCCTG
    GCGAGCTCTG AACCTCCTGC TCTGACCAGA AGCACCAGCC AGGACTCAGA GGCCAGCACA
    GTGGTAAGTA ACAGCTCAGG TGAGACGCTC GGTGCAGACA GTGACCTGAG CAGCACTGCT
    GGAGATGGCC TGACAGGAAG ACATGGTCAG CATCTCAACC TGTCCCGTGG CACACTGTCT
    GACAGTGAGA TTGAGACCAA CCCTGCAACC AGCTCAGTGT TTGGCAAGAC ACACAAGCTG
    AAGCCAGGAG TGAAGGAGCC TGTTGGTGTT AATAAAGCAG CACCTGCTCC ACCCGTTGAG
    GATGTTAGCA TGAGGATCTA TCTGTGTGAG GGGCTTCTGG GCCGGGATAA AAGCTCAGTT
    TGGGACCAGT TGGAGGATGC TGCAATGGAA ACCTTTTCTC TGAGTAAAGA GCGCTCAACT
    CTGTGGGATC AGATGCAGTT CTGGGAGGAC GCCTTCCTGG ATGCCGTAAT GCTGGAACGG
    GAAGGAATGG GAATGGATCA GGGACCACAA GAAATGATTG ACAGGTATCT TTCTCTGGGA
    GATCATGACC GCAAGAGACT GGAGGATGAT GAGGACAGAC TGCTGGCCAC GCTTCTGCAC
    AATATGATTG CTTACATGCT TATGATGAAG GTCGGTAAAA ACGACATCAG GAAGAAAGTA
    CGTCGTCTTA TGGGCAAATC CCACATTGGC CTCACTCACA GTCAGGAGAT CAATGAAGTC
    CTGGATCGCC TCGCACATTT AAGCGGTCGG GAGCTTTCTA TCAGGCCGAG TGGCAGCAGG
    CATATTAAAA AACAGACATT TGTTGTGCAT GCTGGGACAG ATACAACTGG TGACATATTC
    TTCATGGAGG TGTGTGATGA TTGCATCGTC TTGCGCAGTA ATATTGGCAC AGTCTATGAG
    CGCTGGTGGT ATGAGAAGCT CATAAATATG ACTTACTGTC CCAAAACTAA AGTGTTGTGT
    CTGTGGAGGA GAAACGGCCA GGAGACGCAG CTAAACAAGT TTTACACTAA GAAGTGTCGG
    GAATTGTACT ACTGTGTTAA AGACAGCATG GAGCGTGCTG CTGCTCGACA ACAAAGCATC
    AAACCAGGTC CAGAGCTTGG AGGCGAGTTT CCGGTTCAGG ACATGAAGAC AGGGGAGGGT
    GGTCTCCTCC AAGTCACACT GGAAGGCATC AACCTCAAAT TTATGCACAG CCAGGAGCGG
    AAGGTTTTCA TAGAGCTGAA TCACATTAAA AAGTGCAATA CAGTGAAAGG AGTCTTTGTC
    CTGGAGGAGT TTGTTCCTGA AACTAAAGAA GTGGTGATCC ATAAGTACAA GACTCCTATG
    GCACATCAGA TCTGTTACTC GGTGCTCTGC CTCTTCTCCT ACATGGCGGC GGTCAAGGGG
    AAAGAGTCAG AGGGCAAACC CAAGATGCTG TCCCCTCGTC CACTAGCGAG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144883
    Clone ID Related Accession (Same CDS sequence) XM_021471953.1
    Accession Version XM_021471953.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5145bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394698.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA CTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGGACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCGGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT TCCCAAGCTC TGCTCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTCCCCAGC CTCAAAGAAT ATTTTAATTT TGATTTAGCA
    GAGGACACAG ATCAAGACGA TGCTGATGAC AGTCCTGAAA GTCCTGTATT TGGGCTAAAT
    TCTCTAATGG AGATTGTAAC AGAGATCGGC CCGGGGAGCG GAGAAGGTGC CCGAGCCCCC
    AGAGCACTGG TAGATCAGAA ATCCTCTGTT ATTAAGCACA GCCCTACTGT GAAGAGAGAG
    TCTCCGTCTC CTCAAGGCAG AGCCAACAAT ACCAGTGAGA ACCAGCAGTT TCTAAAGGAG
    GTGGTCCAGA GCGTGCTGGA GGGGCAGGGT GTGGGCTGGC TTAACATGAA GAAGGTGCGT
    CGCCTGCTAG AGAATGAACA GCTCAGAGTC TTTGTGCTCA GCAAACTCAA CCGTGCGGTC
    CAGTCAGAGG AGGACGCCCA GCAGGAAGTT ATAAGAGATG TGGAGATAAA TAGAAAGGTC
    TACAAAGGCA TGCTGGATAT TTTGAAGTGC ACGGTGTCCA GTTTGGAGCA TTCATACACT
    AATGCTGGCT TAGGGGGCAT GGCCAGTGTC TTCAGTCTGC TAGAGATAGC ACGCACCCAC
    TACCAAACCA AAGATCCAGA GAAGCGGAAA CGGAGCCCCA CCGAAGGGCT TAGCAGTCCA
    GGCAGTAAGG AGAGCCCATC GGGCCGAATG GAGAGTGCCA GGGCGGCAGG GGTGCTTCTG
    GTACCCCGTA TCCAATTGCC GCCACCCTCC TCTGGGAAAT CATCACGCCA GTTTGATACC
    CGGAGTCTGA ACGAGGAGAA TTTTATTGCC TCCATTGAAT TGTGGAGCAA GCACCAGGAT
    AACAGAAAGC AAAAAGCTTT GGAAAAAGAA CAGAGTGGTT TAAATAAACG GAAATCAGGA
    GCGGATGGAG TAAAACAACG TGTAGAAGGA GGAGACACTG AGGAGAAGAA ATCACAGATC
    AGTGCAGACA GTGGCCTCAG TGTGACATCT GGCTCACAGA AGAGTGATAC GGATTCCCTG
    GCGAGCTCTG AACCTCCTGC TCTGACCAGA AGCACCAGCC AGGACTCAGA GGCCAGCACA
    GTGGTAAGTA ACAGCTCAGG TGAGACGCTC GGTGCAGACA GTGACCTGAG CAGCACTGCT
    GGAGATGGCC TGACAGGAAG ACATGGTCAG CATCTCAACC TGTCCCGTGG CACACTGTCT
    GACAGTGAGA TTGAGACCAA CCCTGCAACC AGCTCAGTGT TTGGCAAGAC ACACAAGCTG
    AAGCCAGGAG TGAAGGAGCC TGTTGGTGTT AATAAAGCAG CACCTGCTCC ACCCGTTGAG
    GATGTTAGCA TGAGGATCTA TCTGTGTGAG GGGCTTCTGG GCCGGGATAA AAGCTCAGTT
    TGGGACCAGT TGGAGGATGC TGCAATGGAA ACCTTTTCTC TGAGTAAAGA GCGCTCAACT
    CTGTGGGATC AGATGCAGTT CTGGGAGGAC GCCTTCCTGG ATGCCGTAAT GCTGGAACGG
    GAAGGAATGG GAATGGATCA GGGACCACAA GAAATGATTG ACAGGTATCT TTCTCTGGGA
    GATCATGACC GCAAGAGACT GGAGGATGAT GAGGACAGAC TGCTGGCCAC GCTTCTGCAC
    AATATGATTG CTTACATGCT TATGATGAAG GTCGGTAAAA ACGACATCAG GAAGAAAGTA
    CGTCGTCTTA TGGGCAAATC CCACATTGGC CTCACTCACA GTCAGGAGAT CAATGAAGTC
    CTGGATCGCC TCGCACATTT AAGCGGTCGG GAGCTTTCTA TCAGGCCGAG TGGCAGCAGG
    CATATTAAAA AACAGACATT TGTTGTGCAT GCTGGGACAG ATACAACTGG TGACATATTC
    TTCATGGAGG TGTGTGATGA TTGCATCGTC TTGCGCAGTA ATATTGGCAC AGTCTATGAG
    CGCTGGTGGT ATGAGAAGCT CATAAATATG ACTTACTGTC CCAAAACTAA AGTGTTGTGT
    CTGTGGAGGA GAAACGGCCA GGAGACGCAG CTAAACAAGT TTTACACTAA GAAGTGTCGG
    GAATTGTACT ACTGTGTTAA AGACAGCATG GAGCGTGCTG CTGCTCGACA ACAAAGCATC
    AAACCAGGTC CAGAGCTTGG AGGCGAGTTT CCGGTTCAGG ACATGAAGAC AGGGGAGGGT
    GGTCTCCTCC AAGTCACACT GGAAGGCATC AACCTCAAAT TTATGCACAG CCAGGTTTTC
    ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTGAAAG GAGTCTTTGT CCTGGAGGAG
    TTTGTTCCTG AAACTAAAGA AGTGGTGATC CATAAGTACA AGACTCCTAT GGCACATCAG
    ATCTGTTACT CGGTGCTCTG CCTCTTCTCC TACATGGCGG CGGTCAAGGG GAAAGAGTCA
    GAGGGCAAAC CCAAGATGCT GTCCCCTCGT CCACTAGCGA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327628.1
    CDS169..5313
    Translation

    Target ORF information:

    RefSeq Version XM_021471953.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X42, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471953.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA CTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGGACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCGGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT TCCCAAGCTC TGCTCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTCCCCAGC CTCAAAGAAT ATTTTAATTT TGATTTAGCA
    GAGGACACAG ATCAAGACGA TGCTGATGAC AGTCCTGAAA GTCCTGTATT TGGGCTAAAT
    TCTCTAATGG AGATTGTAAC AGAGATCGGC CCGGGGAGCG GAGAAGGTGC CCGAGCCCCC
    AGAGCACTGG TAGATCAGAA ATCCTCTGTT ATTAAGCACA GCCCTACTGT GAAGAGAGAG
    TCTCCGTCTC CTCAAGGCAG AGCCAACAAT ACCAGTGAGA ACCAGCAGTT TCTAAAGGAG
    GTGGTCCAGA GCGTGCTGGA GGGGCAGGGT GTGGGCTGGC TTAACATGAA GAAGGTGCGT
    CGCCTGCTAG AGAATGAACA GCTCAGAGTC TTTGTGCTCA GCAAACTCAA CCGTGCGGTC
    CAGTCAGAGG AGGACGCCCA GCAGGAAGTT ATAAGAGATG TGGAGATAAA TAGAAAGGTC
    TACAAAGGCA TGCTGGATAT TTTGAAGTGC ACGGTGTCCA GTTTGGAGCA TTCATACACT
    AATGCTGGCT TAGGGGGCAT GGCCAGTGTC TTCAGTCTGC TAGAGATAGC ACGCACCCAC
    TACCAAACCA AAGATCCAGA GAAGCGGAAA CGGAGCCCCA CCGAAGGGCT TAGCAGTCCA
    GGCAGTAAGG AGAGCCCATC GGGCCGAATG GAGAGTGCCA GGGCGGCAGG GGTGCTTCTG
    GTACCCCGTA TCCAATTGCC GCCACCCTCC TCTGGGAAAT CATCACGCCA GTTTGATACC
    CGGAGTCTGA ACGAGGAGAA TTTTATTGCC TCCATTGAAT TGTGGAGCAA GCACCAGGAT
    AACAGAAAGC AAAAAGCTTT GGAAAAAGAA CAGAGTGGTT TAAATAAACG GAAATCAGGA
    GCGGATGGAG TAAAACAACG TGTAGAAGGA GGAGACACTG AGGAGAAGAA ATCACAGATC
    AGTGCAGACA GTGGCCTCAG TGTGACATCT GGCTCACAGA AGAGTGATAC GGATTCCCTG
    GCGAGCTCTG AACCTCCTGC TCTGACCAGA AGCACCAGCC AGGACTCAGA GGCCAGCACA
    GTGGTAAGTA ACAGCTCAGG TGAGACGCTC GGTGCAGACA GTGACCTGAG CAGCACTGCT
    GGAGATGGCC TGACAGGAAG ACATGGTCAG CATCTCAACC TGTCCCGTGG CACACTGTCT
    GACAGTGAGA TTGAGACCAA CCCTGCAACC AGCTCAGTGT TTGGCAAGAC ACACAAGCTG
    AAGCCAGGAG TGAAGGAGCC TGTTGGTGTT AATAAAGCAG CACCTGCTCC ACCCGTTGAG
    GATGTTAGCA TGAGGATCTA TCTGTGTGAG GGGCTTCTGG GCCGGGATAA AAGCTCAGTT
    TGGGACCAGT TGGAGGATGC TGCAATGGAA ACCTTTTCTC TGAGTAAAGA GCGCTCAACT
    CTGTGGGATC AGATGCAGTT CTGGGAGGAC GCCTTCCTGG ATGCCGTAAT GCTGGAACGG
    GAAGGAATGG GAATGGATCA GGGACCACAA GAAATGATTG ACAGGTATCT TTCTCTGGGA
    GATCATGACC GCAAGAGACT GGAGGATGAT GAGGACAGAC TGCTGGCCAC GCTTCTGCAC
    AATATGATTG CTTACATGCT TATGATGAAG GTCGGTAAAA ACGACATCAG GAAGAAAGTA
    CGTCGTCTTA TGGGCAAATC CCACATTGGC CTCACTCACA GTCAGGAGAT CAATGAAGTC
    CTGGATCGCC TCGCACATTT AAGCGGTCGG GAGCTTTCTA TCAGGCCGAG TGGCAGCAGG
    CATATTAAAA AACAGACATT TGTTGTGCAT GCTGGGACAG ATACAACTGG TGACATATTC
    TTCATGGAGG TGTGTGATGA TTGCATCGTC TTGCGCAGTA ATATTGGCAC AGTCTATGAG
    CGCTGGTGGT ATGAGAAGCT CATAAATATG ACTTACTGTC CCAAAACTAA AGTGTTGTGT
    CTGTGGAGGA GAAACGGCCA GGAGACGCAG CTAAACAAGT TTTACACTAA GAAGTGTCGG
    GAATTGTACT ACTGTGTTAA AGACAGCATG GAGCGTGCTG CTGCTCGACA ACAAAGCATC
    AAACCAGGTC CAGAGCTTGG AGGCGAGTTT CCGGTTCAGG ACATGAAGAC AGGGGAGGGT
    GGTCTCCTCC AAGTCACACT GGAAGGCATC AACCTCAAAT TTATGCACAG CCAGGTTTTC
    ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTGAAAG GAGTCTTTGT CCTGGAGGAG
    TTTGTTCCTG AAACTAAAGA AGTGGTGATC CATAAGTACA AGACTCCTAT GGCACATCAG
    ATCTGTTACT CGGTGCTCTG CCTCTTCTCC TACATGGCGG CGGTCAAGGG GAAAGAGTCA
    GAGGGCAAAC CCAAGATGCT GTCCCCTCGT CCACTAGCGA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144884
    Clone ID Related Accession (Same CDS sequence) XM_021471954.1
    Accession Version XM_021471954.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5145bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394698.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGTG TCTGGTAAGG
    TTGACTCTGA TCACCCAAAA GCAGATCTTC GCCTCCGATT CTAAGGCTCT GGCTAGCATG
    AGTCTAAACA CACAGCCTAT TCTGAACCTG GAGAAGTTTC AGGATGGACA GGAGTTGTCT
    TTATTGCCCC CCGGTCGTGA CAAAGCTTCC CCGTCCTCCA CTGAGTTCAA CCCACTCATC
    TACGGCAATG ATGTGGACTC AGTTGATGTT GCCACAAGGG TGGCAATGGT GAGGTTCTTC
    AACTCTCCAA ACGTGCTGCA AGGGTTCCAG ATGCACACAC GCACTCTCCG TCTCTTCCCT
    CGTCCTGTGG TGGCCTTTCA GTCCACATCT TTCCTGGCTT CTAGGCCACG ACGAAATGGA
    TTCACAGAAA AGCTCTCCCA CACTCAGGCA GTGGAGTACT ATGGAGAATG GGCTCTCAAC
    CCCACCAACC TGGCTTTCCA AAGGATTCAT AACAATGTGT ATGACCCCTC TCTAATAGGC
    GATAAGCCTA AGTGGTATGC CCACCAGTTA CAGCCAGTAT TCTACCGCGT GTATGATGGA
    AACTCTCATT TAGCGGAGGC CTTGAGTGGA CCCCTGCAGG ATGAGACCAA TGATTCAGAC
    CCAACAGACG ACAGTGGCAG CGACAGTGAA GCATATGATG ACTCCAGCTC CTCCTACTCA
    TCTCTTGGAG ACTTTGTGAA TGAGATGATC AAAGGGGACA TACAGGGAGA CACACCAAAT
    GTTGATCCCC TGACCCATGC TGCACTGGGA GATGCAGATG AGGTTGAGAT CCATGACTTT
    CACGAGTATA AGGGGGACAG CGGTGAACCA GAGCCAGAAG GCCCCACAGA AGCGGCGGAC
    AGTCAGCCCT TGCGGTCCAG CTCTAGCACC ACAGCCAGTT CCAGCCCCAG TACAGTCATA
    CAGGGAGTCA ACCATGAGCA GAAGGAACCT GTTGAGGTGG AGCCCATAGC AAATGTGACT
    TTCCCAAGCT CTGCTCCAGT ATTGGGTCCT CCTCCTTTTA CAAGACCCAC CCCTGATCCA
    ACCCTTGTTG ACCCTGCCAA TAAGAAGCGA GAATATGACA ACCCCTATTT TGAGCCCCAG
    TACGGTTTCC CTACAGAGGA AGATGCTGAG ACTGATGAGC AGGAGGAGAG TTACACACCA
    CGATTTAACC AGAATCTCAA TGGCAATAAG CCTTCTCGTC CACTGAGACC CAGCAGCCTG
    AAGCTTCCGG GTGAATCTGA CGGAGAGGGA GACTCCAGAA ATAGCTCTCC TAACTCAACC
    ATCTCCAACA ATAGCAGTGA TGGCTTTGGG GGACTCATGT CCTTTGCCAG TAACTTGTAT
    AAAAACCATG GCACCAGTTT CAGTCTGTCC AGCCTGGCTC TGCCTAATAA AGCGAGAGAG
    AAGAACACGC CGTTCCCCAG CCTCAAAGAA TATTTTAATT TTGATTTAGC AGAGGACACA
    GATCAAGACG ATGCTGATGA CAGTCCTGAA AGTCCTGTAT TTGGGCTAAA TTCTCTAATG
    GAGATTGTAA CAGAGATCGG CCCGGGGAGC GGAGAAGGTG CCCGAGCCCC CAGAGCACTG
    GTAGATCAGA AATCCTCTGT TATTAAGCAC AGCCCTACTG TGAAGAGAGA GTCTCCGTCT
    CCTCAAGGCA GAGCCAACAA TACCAGTGAG AACCAGCAGT TTCTAAAGGA GGTGGTCCAG
    AGCGTGCTGG AGGGGCAGGG TGTGGGCTGG CTTAACATGA AGAAGGTGCG TCGCCTGCTA
    GAGAATGAAC AGCTCAGAGT CTTTGTGCTC AGCAAACTCA ACCGTGCGGT CCAGTCAGAG
    GAGGACGCCC AGCAGGAAGT TATAAGAGAT GTGGAGATAA ATAGAAAGGT CTACAAAGGC
    ATGCTGGATA TTTTGAAGTG CACGGTGTCC AGTTTGGAGC ATTCATACAC TAATGCTGGC
    TTAGGGGGCA TGGCCAGTGT CTTCAGTCTG CTAGAGATAG CACGCACCCA CTACCAAACC
    AAAGATCCAG AGAAGCGGAA ACGGAGCCCC ACCGAAGGGC TTAGCAGTCC AGGCAGTAAG
    GAGAGCCCAT CGGGCCGAAT GGAGAGTGCC AGGGCGGCAG GGGTGCTTCT GGTACCCCGT
    ATCCAATTGC CGCCACCCTC CTCTGGGAAA TCATCACGCC AGTTTGATAC CCGGAGTCTG
    AACGAGGAGA ATTTTATTGC CTCCATTGAA TTGTGGAGCA AGCACCAGGA TAACAGAAAG
    CAAAAAGCTT TGGAAAAAGA ACAGAGTGGT TTAAATAAAC GGAAATCAGG AGCGGATGGA
    GTAAAACAAC GTGTAGAAGG AGGAGACACT GAGGAGAAGA AATCACAGAT CAGTGCAGAC
    AGTGGCCTCA GTGTGACATC TGGCTCACAG AAGAGTGATA CGGATTCCCT GGCGAGCTCT
    GAACCTCCTG CTCTGACCAG AAGCACCAGC CAGGACTCAG AGGCCAGCAC AGTGGTAAGT
    AACAGCTCAG GTGAGACGCT CGGTGCAGAC AGTGACCTGA GCAGCACTGC TGGAGATGGC
    CTGACAGGAA GACATGGTCA GCATCTCAAC CTGTCCCGTG GCACACTGTC TGACAGTGAG
    ATTGAGACCA ACCCTGCAAC CAGCTCAGTG TTTGGCAAGA CACACAAGCT GAAGCCAGGA
    GTGAAGGAGC CTGTTGGTGT TAATAAAGCA GCACCTGCTC CACCCGTTGA GGATGTTAGC
    ATGAGGATCT ATCTGTGTGA GGGGCTTCTG GGCCGGGATA AAAGCTCAGT TTGGGACCAG
    TTGGAGGATG CTGCAATGGA AACCTTTTCT CTGAGTAAAG AGCGCTCAAC TCTGTGGGAT
    CAGATGCAGT TCTGGGAGGA CGCCTTCCTG GATGCCGTAA TGCTGGAACG GGAAGGAATG
    GGAATGGATC AGGGACCACA AGAAATGATT GACAGGTATC TTTCTCTGGG AGATCATGAC
    CGCAAGAGAC TGGAGGATGA TGAGGACAGA CTGCTGGCCA CGCTTCTGCA CAATATGATT
    GCTTACATGC TTATGATGAA GGTCGGTAAA AACGACATCA GGAAGAAAGT ACGTCGTCTT
    ATGGGCAAAT CCCACATTGG CCTCACTCAC AGTCAGGAGA TCAATGAAGT CCTGGATCGC
    CTCGCACATT TAAGCGGTCG GGAGCTTTCT ATCAGGCCGA GTGGCAGCAG GCATATTAAA
    AAACAGACAT TTGTTGTGCA TGCTGGGACA GATACAACTG GTGACATATT CTTCATGGAG
    GTGTGTGATG ATTGCATCGT CTTGCGCAGT AATATTGGCA CAGTCTATGA GCGCTGGTGG
    TATGAGAAGC TCATAAATAT GACTTACTGT CCCAAAACTA AAGTGTTGTG TCTGTGGAGG
    AGAAACGGCC AGGAGACGCA GCTAAACAAG TTTTACACTA AGAAGTGTCG GGAATTGTAC
    TACTGTGTTA AAGACAGCAT GGAGCGTGCT GCTGCTCGAC AACAAAGCAT CAAACCAGGT
    CCAGAGCTTG GAGGCGAGTT TCCGGTTCAG GACATGAAGA CAGGGGAGGG TGGTCTCCTC
    CAAGTCACAC TGGAAGGCAT CAACCTCAAA TTTATGCACA GCCAGGAGCG GAAGGTTTTC
    ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTGAAAG GAGTCTTTGT CCTGGAGGAG
    TTTGTTCCTG AAACTAAAGA AGTGGTGATC CATAAGTACA AGACTCCTAT GGCACATCAG
    ATCTGTTACT CGGTGCTCTG CCTCTTCTCC TACATGGCGG CGGTCAAGGG GAAAGAGTCA
    GAGGGCAAAC CCAAGATGCT GTCCCCTCGT CCACTAGCGA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327629.1
    CDS169..5313
    Translation

    Target ORF information:

    RefSeq Version XM_021471954.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X43, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471954.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGTG TCTGGTAAGG
    TTGACTCTGA TCACCCAAAA GCAGATCTTC GCCTCCGATT CTAAGGCTCT GGCTAGCATG
    AGTCTAAACA CACAGCCTAT TCTGAACCTG GAGAAGTTTC AGGATGGACA GGAGTTGTCT
    TTATTGCCCC CCGGTCGTGA CAAAGCTTCC CCGTCCTCCA CTGAGTTCAA CCCACTCATC
    TACGGCAATG ATGTGGACTC AGTTGATGTT GCCACAAGGG TGGCAATGGT GAGGTTCTTC
    AACTCTCCAA ACGTGCTGCA AGGGTTCCAG ATGCACACAC GCACTCTCCG TCTCTTCCCT
    CGTCCTGTGG TGGCCTTTCA GTCCACATCT TTCCTGGCTT CTAGGCCACG ACGAAATGGA
    TTCACAGAAA AGCTCTCCCA CACTCAGGCA GTGGAGTACT ATGGAGAATG GGCTCTCAAC
    CCCACCAACC TGGCTTTCCA AAGGATTCAT AACAATGTGT ATGACCCCTC TCTAATAGGC
    GATAAGCCTA AGTGGTATGC CCACCAGTTA CAGCCAGTAT TCTACCGCGT GTATGATGGA
    AACTCTCATT TAGCGGAGGC CTTGAGTGGA CCCCTGCAGG ATGAGACCAA TGATTCAGAC
    CCAACAGACG ACAGTGGCAG CGACAGTGAA GCATATGATG ACTCCAGCTC CTCCTACTCA
    TCTCTTGGAG ACTTTGTGAA TGAGATGATC AAAGGGGACA TACAGGGAGA CACACCAAAT
    GTTGATCCCC TGACCCATGC TGCACTGGGA GATGCAGATG AGGTTGAGAT CCATGACTTT
    CACGAGTATA AGGGGGACAG CGGTGAACCA GAGCCAGAAG GCCCCACAGA AGCGGCGGAC
    AGTCAGCCCT TGCGGTCCAG CTCTAGCACC ACAGCCAGTT CCAGCCCCAG TACAGTCATA
    CAGGGAGTCA ACCATGAGCA GAAGGAACCT GTTGAGGTGG AGCCCATAGC AAATGTGACT
    TTCCCAAGCT CTGCTCCAGT ATTGGGTCCT CCTCCTTTTA CAAGACCCAC CCCTGATCCA
    ACCCTTGTTG ACCCTGCCAA TAAGAAGCGA GAATATGACA ACCCCTATTT TGAGCCCCAG
    TACGGTTTCC CTACAGAGGA AGATGCTGAG ACTGATGAGC AGGAGGAGAG TTACACACCA
    CGATTTAACC AGAATCTCAA TGGCAATAAG CCTTCTCGTC CACTGAGACC CAGCAGCCTG
    AAGCTTCCGG GTGAATCTGA CGGAGAGGGA GACTCCAGAA ATAGCTCTCC TAACTCAACC
    ATCTCCAACA ATAGCAGTGA TGGCTTTGGG GGACTCATGT CCTTTGCCAG TAACTTGTAT
    AAAAACCATG GCACCAGTTT CAGTCTGTCC AGCCTGGCTC TGCCTAATAA AGCGAGAGAG
    AAGAACACGC CGTTCCCCAG CCTCAAAGAA TATTTTAATT TTGATTTAGC AGAGGACACA
    GATCAAGACG ATGCTGATGA CAGTCCTGAA AGTCCTGTAT TTGGGCTAAA TTCTCTAATG
    GAGATTGTAA CAGAGATCGG CCCGGGGAGC GGAGAAGGTG CCCGAGCCCC CAGAGCACTG
    GTAGATCAGA AATCCTCTGT TATTAAGCAC AGCCCTACTG TGAAGAGAGA GTCTCCGTCT
    CCTCAAGGCA GAGCCAACAA TACCAGTGAG AACCAGCAGT TTCTAAAGGA GGTGGTCCAG
    AGCGTGCTGG AGGGGCAGGG TGTGGGCTGG CTTAACATGA AGAAGGTGCG TCGCCTGCTA
    GAGAATGAAC AGCTCAGAGT CTTTGTGCTC AGCAAACTCA ACCGTGCGGT CCAGTCAGAG
    GAGGACGCCC AGCAGGAAGT TATAAGAGAT GTGGAGATAA ATAGAAAGGT CTACAAAGGC
    ATGCTGGATA TTTTGAAGTG CACGGTGTCC AGTTTGGAGC ATTCATACAC TAATGCTGGC
    TTAGGGGGCA TGGCCAGTGT CTTCAGTCTG CTAGAGATAG CACGCACCCA CTACCAAACC
    AAAGATCCAG AGAAGCGGAA ACGGAGCCCC ACCGAAGGGC TTAGCAGTCC AGGCAGTAAG
    GAGAGCCCAT CGGGCCGAAT GGAGAGTGCC AGGGCGGCAG GGGTGCTTCT GGTACCCCGT
    ATCCAATTGC CGCCACCCTC CTCTGGGAAA TCATCACGCC AGTTTGATAC CCGGAGTCTG
    AACGAGGAGA ATTTTATTGC CTCCATTGAA TTGTGGAGCA AGCACCAGGA TAACAGAAAG
    CAAAAAGCTT TGGAAAAAGA ACAGAGTGGT TTAAATAAAC GGAAATCAGG AGCGGATGGA
    GTAAAACAAC GTGTAGAAGG AGGAGACACT GAGGAGAAGA AATCACAGAT CAGTGCAGAC
    AGTGGCCTCA GTGTGACATC TGGCTCACAG AAGAGTGATA CGGATTCCCT GGCGAGCTCT
    GAACCTCCTG CTCTGACCAG AAGCACCAGC CAGGACTCAG AGGCCAGCAC AGTGGTAAGT
    AACAGCTCAG GTGAGACGCT CGGTGCAGAC AGTGACCTGA GCAGCACTGC TGGAGATGGC
    CTGACAGGAA GACATGGTCA GCATCTCAAC CTGTCCCGTG GCACACTGTC TGACAGTGAG
    ATTGAGACCA ACCCTGCAAC CAGCTCAGTG TTTGGCAAGA CACACAAGCT GAAGCCAGGA
    GTGAAGGAGC CTGTTGGTGT TAATAAAGCA GCACCTGCTC CACCCGTTGA GGATGTTAGC
    ATGAGGATCT ATCTGTGTGA GGGGCTTCTG GGCCGGGATA AAAGCTCAGT TTGGGACCAG
    TTGGAGGATG CTGCAATGGA AACCTTTTCT CTGAGTAAAG AGCGCTCAAC TCTGTGGGAT
    CAGATGCAGT TCTGGGAGGA CGCCTTCCTG GATGCCGTAA TGCTGGAACG GGAAGGAATG
    GGAATGGATC AGGGACCACA AGAAATGATT GACAGGTATC TTTCTCTGGG AGATCATGAC
    CGCAAGAGAC TGGAGGATGA TGAGGACAGA CTGCTGGCCA CGCTTCTGCA CAATATGATT
    GCTTACATGC TTATGATGAA GGTCGGTAAA AACGACATCA GGAAGAAAGT ACGTCGTCTT
    ATGGGCAAAT CCCACATTGG CCTCACTCAC AGTCAGGAGA TCAATGAAGT CCTGGATCGC
    CTCGCACATT TAAGCGGTCG GGAGCTTTCT ATCAGGCCGA GTGGCAGCAG GCATATTAAA
    AAACAGACAT TTGTTGTGCA TGCTGGGACA GATACAACTG GTGACATATT CTTCATGGAG
    GTGTGTGATG ATTGCATCGT CTTGCGCAGT AATATTGGCA CAGTCTATGA GCGCTGGTGG
    TATGAGAAGC TCATAAATAT GACTTACTGT CCCAAAACTA AAGTGTTGTG TCTGTGGAGG
    AGAAACGGCC AGGAGACGCA GCTAAACAAG TTTTACACTA AGAAGTGTCG GGAATTGTAC
    TACTGTGTTA AAGACAGCAT GGAGCGTGCT GCTGCTCGAC AACAAAGCAT CAAACCAGGT
    CCAGAGCTTG GAGGCGAGTT TCCGGTTCAG GACATGAAGA CAGGGGAGGG TGGTCTCCTC
    CAAGTCACAC TGGAAGGCAT CAACCTCAAA TTTATGCACA GCCAGGAGCG GAAGGTTTTC
    ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTGAAAG GAGTCTTTGT CCTGGAGGAG
    TTTGTTCCTG AAACTAAAGA AGTGGTGATC CATAAGTACA AGACTCCTAT GGCACATCAG
    ATCTGTTACT CGGTGCTCTG CCTCTTCTCC TACATGGCGG CGGTCAAGGG GAAAGAGTCA
    GAGGGCAAAC CCAAGATGCT GTCCCCTCGT CCACTAGCGA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144885
    Clone ID Related Accession (Same CDS sequence) XM_021471955.1
    Accession Version XM_021471955.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5130bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394698.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA CTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGGACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCGGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT TCCCAAGCTC TGCTCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTCCCCAGC CTCAAAGAAT ATTTTAATTT TGATTTAGCA
    GAGGACACAG ATCAAGACGA TGCTGATGAC AGTCCTGAAA GTCCTGTATT TGGGCTAAAT
    TCTCTAATGG AGATTGTAAC AGAGATCGGC CCGGGGAGCG GAGAAGGTGC CCGAGCCCCC
    AGAGCACTGG TAGATCAGAA ATCCTCTGTT ATTAAGCACA GCCCTACTGT GAAGAGAGAG
    TCTCCGTCTC CTCAAGGCAG AGCCAACAAT ACCAGTGAGA ACCAGCAGTT TCTAAAGGAG
    GTGGTCCAGA GCGTGCTGGA GGGGCAGGGT GTGGGCTGGC TTAACATGAA GAAGGTGCGT
    CGCCTGCTAG AGAATGAACA GCTCAGAGTC TTTGTGCTCA GCAAACTCAA CCGTGCGGTC
    CAGTCAGAGG AGGACGCCCA GCAGGAAGTT ATAAGAGATG TGGAGATAAA TAGAAAGGTC
    TACAAAGGCA TGCTGGATAT TTTGAAGTGC ACGGTGTCCA GTTTGGAGCA TTCATACACT
    AATGCTGGCT TAGGGGGCAT GGCCAGTGTC TTCAGTCTGC TAGAGATAGC ACGCACCCAC
    TACCAAACCA AAGATCCAGA GAAGCGGAAA CGGAGCCCCA CCGAAGGGCT TAGCAGTCCA
    GGCAGTAAGG AGAGCCCATC GGGCCGAATG GAGAGTGCCA GGGCGGCAGG GGTGCTTCTG
    GTACCCCGTA TCCAATTGCC GCCACCCTCC TCTGGGAAAT CATCACGCCA GTTTGATACC
    CGGAGTCTGA ACGAGGAGAA TTTTATTGCC TCCATTGAAT TGTGGAGCAA GCACCAGGAT
    AACAGAAAGC AAAAAGCTTT GGAAAAAGAA CAGAGAGCGG ATGGAGTAAA ACAACGTGTA
    GAAGGAGGAG ACACTGAGGA GAAGAAATCA CAGATCAGTG CAGACAGTGG CCTCAGTGTG
    ACATCTGGCT CACAGAAGAG TGATACGGAT TCCCTGGCGA GCTCTGAACC TCCTGCTCTG
    ACCAGAAGCA CCAGCCAGGA CTCAGAGGCC AGCACAGTGG TAAGTAACAG CTCAGGTGAG
    ACGCTCGGTG CAGACAGTGA CCTGAGCAGC ACTGCTGGAG ATGGCCTGAC AGGAAGACAT
    GGTCAGCATC TCAACCTGTC CCGTGGCACA CTGTCTGACA GTGAGATTGA GACCAACCCT
    GCAACCAGCT CAGTGTTTGG CAAGACACAC AAGCTGAAGC CAGGAGTGAA GGAGCCTGTT
    GGTGTTAATA AAGCAGCACC TGCTCCACCC GTTGAGGATG TTAGCATGAG GATCTATCTG
    TGTGAGGGGC TTCTGGGCCG GGATAAAAGC TCAGTTTGGG ACCAGTTGGA GGATGCTGCA
    ATGGAAACCT TTTCTCTGAG TAAAGAGCGC TCAACTCTGT GGGATCAGAT GCAGTTCTGG
    GAGGACGCCT TCCTGGATGC CGTAATGCTG GAACGGGAAG GAATGGGAAT GGATCAGGGA
    CCACAAGAAA TGATTGACAG GTATCTTTCT CTGGGAGATC ATGACCGCAA GAGACTGGAG
    GATGATGAGG ACAGACTGCT GGCCACGCTT CTGCACAATA TGATTGCTTA CATGCTTATG
    ATGAAGGTCG GTAAAAACGA CATCAGGAAG AAAGTACGTC GTCTTATGGG CAAATCCCAC
    ATTGGCCTCA CTCACAGTCA GGAGATCAAT GAAGTCCTGG ATCGCCTCGC ACATTTAAGC
    GGTCGGGAGC TTTCTATCAG GCCGAGTGGC AGCAGGCATA TTAAAAAACA GACATTTGTT
    GTGCATGCTG GGACAGATAC AACTGGTGAC ATATTCTTCA TGGAGGTGTG TGATGATTGC
    ATCGTCTTGC GCAGTAATAT TGGCACAGTC TATGAGCGCT GGTGGTATGA GAAGCTCATA
    AATATGACTT ACTGTCCCAA AACTAAAGTG TTGTGTCTGT GGAGGAGAAA CGGCCAGGAG
    ACGCAGCTAA ACAAGTTTTA CACTAAGAAG TGTCGGGAAT TGTACTACTG TGTTAAAGAC
    AGCATGGAGC GTGCTGCTGC TCGACAACAA AGCATCAAAC CAGGTCCAGA GCTTGGAGGC
    GAGTTTCCGG TTCAGGACAT GAAGACAGGG GAGGGTGGTC TCCTCCAAGT CACACTGGAA
    GGCATCAACC TCAAATTTAT GCACAGCCAG GAGCGGAAGG TTTTCATAGA GCTGAATCAC
    ATTAAAAAGT GCAATACAGT GAAAGGAGTC TTTGTCCTGG AGGAGTTTGT TCCTGAAACT
    AAAGAAGTGG TGATCCATAA GTACAAGACT CCTATGGCAC ATCAGATCTG TTACTCGGTG
    CTCTGCCTCT TCTCCTACAT GGCGGCGGTC AAGGGGAAAG AGTCAGAGGG CAAACCCAAG
    ATGCTGTCCC CTCGTCCACT AGCGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327630.1
    CDS169..5298
    Translation

    Target ORF information:

    RefSeq Version XM_021471955.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X44, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471955.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA CTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGGACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCGGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT TCCCAAGCTC TGCTCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTCCCCAGC CTCAAAGAAT ATTTTAATTT TGATTTAGCA
    GAGGACACAG ATCAAGACGA TGCTGATGAC AGTCCTGAAA GTCCTGTATT TGGGCTAAAT
    TCTCTAATGG AGATTGTAAC AGAGATCGGC CCGGGGAGCG GAGAAGGTGC CCGAGCCCCC
    AGAGCACTGG TAGATCAGAA ATCCTCTGTT ATTAAGCACA GCCCTACTGT GAAGAGAGAG
    TCTCCGTCTC CTCAAGGCAG AGCCAACAAT ACCAGTGAGA ACCAGCAGTT TCTAAAGGAG
    GTGGTCCAGA GCGTGCTGGA GGGGCAGGGT GTGGGCTGGC TTAACATGAA GAAGGTGCGT
    CGCCTGCTAG AGAATGAACA GCTCAGAGTC TTTGTGCTCA GCAAACTCAA CCGTGCGGTC
    CAGTCAGAGG AGGACGCCCA GCAGGAAGTT ATAAGAGATG TGGAGATAAA TAGAAAGGTC
    TACAAAGGCA TGCTGGATAT TTTGAAGTGC ACGGTGTCCA GTTTGGAGCA TTCATACACT
    AATGCTGGCT TAGGGGGCAT GGCCAGTGTC TTCAGTCTGC TAGAGATAGC ACGCACCCAC
    TACCAAACCA AAGATCCAGA GAAGCGGAAA CGGAGCCCCA CCGAAGGGCT TAGCAGTCCA
    GGCAGTAAGG AGAGCCCATC GGGCCGAATG GAGAGTGCCA GGGCGGCAGG GGTGCTTCTG
    GTACCCCGTA TCCAATTGCC GCCACCCTCC TCTGGGAAAT CATCACGCCA GTTTGATACC
    CGGAGTCTGA ACGAGGAGAA TTTTATTGCC TCCATTGAAT TGTGGAGCAA GCACCAGGAT
    AACAGAAAGC AAAAAGCTTT GGAAAAAGAA CAGAGAGCGG ATGGAGTAAA ACAACGTGTA
    GAAGGAGGAG ACACTGAGGA GAAGAAATCA CAGATCAGTG CAGACAGTGG CCTCAGTGTG
    ACATCTGGCT CACAGAAGAG TGATACGGAT TCCCTGGCGA GCTCTGAACC TCCTGCTCTG
    ACCAGAAGCA CCAGCCAGGA CTCAGAGGCC AGCACAGTGG TAAGTAACAG CTCAGGTGAG
    ACGCTCGGTG CAGACAGTGA CCTGAGCAGC ACTGCTGGAG ATGGCCTGAC AGGAAGACAT
    GGTCAGCATC TCAACCTGTC CCGTGGCACA CTGTCTGACA GTGAGATTGA GACCAACCCT
    GCAACCAGCT CAGTGTTTGG CAAGACACAC AAGCTGAAGC CAGGAGTGAA GGAGCCTGTT
    GGTGTTAATA AAGCAGCACC TGCTCCACCC GTTGAGGATG TTAGCATGAG GATCTATCTG
    TGTGAGGGGC TTCTGGGCCG GGATAAAAGC TCAGTTTGGG ACCAGTTGGA GGATGCTGCA
    ATGGAAACCT TTTCTCTGAG TAAAGAGCGC TCAACTCTGT GGGATCAGAT GCAGTTCTGG
    GAGGACGCCT TCCTGGATGC CGTAATGCTG GAACGGGAAG GAATGGGAAT GGATCAGGGA
    CCACAAGAAA TGATTGACAG GTATCTTTCT CTGGGAGATC ATGACCGCAA GAGACTGGAG
    GATGATGAGG ACAGACTGCT GGCCACGCTT CTGCACAATA TGATTGCTTA CATGCTTATG
    ATGAAGGTCG GTAAAAACGA CATCAGGAAG AAAGTACGTC GTCTTATGGG CAAATCCCAC
    ATTGGCCTCA CTCACAGTCA GGAGATCAAT GAAGTCCTGG ATCGCCTCGC ACATTTAAGC
    GGTCGGGAGC TTTCTATCAG GCCGAGTGGC AGCAGGCATA TTAAAAAACA GACATTTGTT
    GTGCATGCTG GGACAGATAC AACTGGTGAC ATATTCTTCA TGGAGGTGTG TGATGATTGC
    ATCGTCTTGC GCAGTAATAT TGGCACAGTC TATGAGCGCT GGTGGTATGA GAAGCTCATA
    AATATGACTT ACTGTCCCAA AACTAAAGTG TTGTGTCTGT GGAGGAGAAA CGGCCAGGAG
    ACGCAGCTAA ACAAGTTTTA CACTAAGAAG TGTCGGGAAT TGTACTACTG TGTTAAAGAC
    AGCATGGAGC GTGCTGCTGC TCGACAACAA AGCATCAAAC CAGGTCCAGA GCTTGGAGGC
    GAGTTTCCGG TTCAGGACAT GAAGACAGGG GAGGGTGGTC TCCTCCAAGT CACACTGGAA
    GGCATCAACC TCAAATTTAT GCACAGCCAG GAGCGGAAGG TTTTCATAGA GCTGAATCAC
    ATTAAAAAGT GCAATACAGT GAAAGGAGTC TTTGTCCTGG AGGAGTTTGT TCCTGAAACT
    AAAGAAGTGG TGATCCATAA GTACAAGACT CCTATGGCAC ATCAGATCTG TTACTCGGTG
    CTCTGCCTCT TCTCCTACAT GGCGGCGGTC AAGGGGAAAG AGTCAGAGGG CAAACCCAAG
    ATGCTGTCCC CTCGTCCACT AGCGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144886
    Clone ID Related Accession (Same CDS sequence) XM_021471956.1
    Accession Version XM_021471956.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5124bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394698.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA CTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGGACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCGGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT TCCCAAGCTC TGCTCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTCCCCAGC CTCAAAGAAT ATTTTAATTT TGATTTAGCA
    GAGGACACAG ATCAAGTATT TGGGCTAAAT TCTCTAATGG AGATTGTAAC AGAGATCGGC
    CCGGGGAGCG GAGAAGGTGC CCGAGCCCCC AGAGCACTGG TAGATCAGAA ATCCTCTGTT
    ATTAAGCACA GCCCTACTGT GAAGAGAGAG TCTCCGTCTC CTCAAGGCAG AGCCAACAAT
    ACCAGTGAGA ACCAGCAGTT TCTAAAGGAG GTGGTCCAGA GCGTGCTGGA GGGGCAGGGT
    GTGGGCTGGC TTAACATGAA GAAGGTGCGT CGCCTGCTAG AGAATGAACA GCTCAGAGTC
    TTTGTGCTCA GCAAACTCAA CCGTGCGGTC CAGTCAGAGG AGGACGCCCA GCAGGAAGTT
    ATAAGAGATG TGGAGATAAA TAGAAAGGTC TACAAAGGCA TGCTGGATAT TTTGAAGTGC
    ACGGTGTCCA GTTTGGAGCA TTCATACACT AATGCTGGCT TAGGGGGCAT GGCCAGTGTC
    TTCAGTCTGC TAGAGATAGC ACGCACCCAC TACCAAACCA AAGATCCAGA GAAGCGGAAA
    CGGAGCCCCA CCGAAGGGCT TAGCAGTCCA GGCAGTAAGG AGAGCCCATC GGGCCGAATG
    GAGAGTGCCA GGGCGGCAGG GGTGCTTCTG GTACCCCGTA TCCAATTGCC GCCACCCTCC
    TCTGGGAAAT CATCACGCCA GTTTGATACC CGGAGTCTGA ACGAGGAGAA TTTTATTGCC
    TCCATTGAAT TGTGGAGCAA GCACCAGGAT AACAGAAAGC AAAAAGCTTT GGAAAAAGAA
    CAGAGTGGTT TAAATAAACG GAAATCAGGA GCGGATGGAG TAAAACAACG TGTAGAAGGA
    GGAGACACTG AGGAGAAGAA ATCACAGATC AGTGCAGACA GTGGCCTCAG TGTGACATCT
    GGCTCACAGA AGAGTGATAC GGATTCCCTG GCGAGCTCTG AACCTCCTGC TCTGACCAGA
    AGCACCAGCC AGGACTCAGA GGCCAGCACA GTGGTAAGTA ACAGCTCAGG TGAGACGCTC
    GGTGCAGACA GTGACCTGAG CAGCACTGCT GGAGATGGCC TGACAGGAAG ACATGGTCAG
    CATCTCAACC TGTCCCGTGG CACACTGTCT GACAGTGAGA TTGAGACCAA CCCTGCAACC
    AGCTCAGTGT TTGGCAAGAC ACACAAGCTG AAGCCAGGAG TGAAGGAGCC TGTTGGTGTT
    AATAAAGCAG CACCTGCTCC ACCCGTTGAG GATGTTAGCA TGAGGATCTA TCTGTGTGAG
    GGGCTTCTGG GCCGGGATAA AAGCTCAGTT TGGGACCAGT TGGAGGATGC TGCAATGGAA
    ACCTTTTCTC TGAGTAAAGA GCGCTCAACT CTGTGGGATC AGATGCAGTT CTGGGAGGAC
    GCCTTCCTGG ATGCCGTAAT GCTGGAACGG GAAGGAATGG GAATGGATCA GGGACCACAA
    GAAATGATTG ACAGGTATCT TTCTCTGGGA GATCATGACC GCAAGAGACT GGAGGATGAT
    GAGGACAGAC TGCTGGCCAC GCTTCTGCAC AATATGATTG CTTACATGCT TATGATGAAG
    GTCGGTAAAA ACGACATCAG GAAGAAAGTA CGTCGTCTTA TGGGCAAATC CCACATTGGC
    CTCACTCACA GTCAGGAGAT CAATGAAGTC CTGGATCGCC TCGCACATTT AAGCGGTCGG
    GAGCTTTCTA TCAGGCCGAG TGGCAGCAGG CATATTAAAA AACAGACATT TGTTGTGCAT
    GCTGGGACAG ATACAACTGG TGACATATTC TTCATGGAGG TGTGTGATGA TTGCATCGTC
    TTGCGCAGTA ATATTGGCAC AGTCTATGAG CGCTGGTGGT ATGAGAAGCT CATAAATATG
    ACTTACTGTC CCAAAACTAA AGTGTTGTGT CTGTGGAGGA GAAACGGCCA GGAGACGCAG
    CTAAACAAGT TTTACACTAA GAAGTGTCGG GAATTGTACT ACTGTGTTAA AGACAGCATG
    GAGCGTGCTG CTGCTCGACA ACAAAGCATC AAACCAGGTC CAGAGCTTGG AGGCGAGTTT
    CCGGTTCAGG ACATGAAGAC AGGGGAGGGT GGTCTCCTCC AAGTCACACT GGAAGGCATC
    AACCTCAAAT TTATGCACAG CCAGGAGCGG AAGGTTTTCA TAGAGCTGAA TCACATTAAA
    AAGTGCAATA CAGTGAAAGG AGTCTTTGTC CTGGAGGAGT TTGTTCCTGA AACTAAAGAA
    GTGGTGATCC ATAAGTACAA GACTCCTATG GCACATCAGA TCTGTTACTC GGTGCTCTGC
    CTCTTCTCCT ACATGGCGGC GGTCAAGGGG AAAGAGTCAG AGGGCAAACC CAAGATGCTG
    TCCCCTCGTC CACTAGCGAG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327631.1
    CDS169..5292
    Translation

    Target ORF information:

    RefSeq Version XM_021471956.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X45, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471956.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA CTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGGACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCGGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT TCCCAAGCTC TGCTCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTCCCCAGC CTCAAAGAAT ATTTTAATTT TGATTTAGCA
    GAGGACACAG ATCAAGTATT TGGGCTAAAT TCTCTAATGG AGATTGTAAC AGAGATCGGC
    CCGGGGAGCG GAGAAGGTGC CCGAGCCCCC AGAGCACTGG TAGATCAGAA ATCCTCTGTT
    ATTAAGCACA GCCCTACTGT GAAGAGAGAG TCTCCGTCTC CTCAAGGCAG AGCCAACAAT
    ACCAGTGAGA ACCAGCAGTT TCTAAAGGAG GTGGTCCAGA GCGTGCTGGA GGGGCAGGGT
    GTGGGCTGGC TTAACATGAA GAAGGTGCGT CGCCTGCTAG AGAATGAACA GCTCAGAGTC
    TTTGTGCTCA GCAAACTCAA CCGTGCGGTC CAGTCAGAGG AGGACGCCCA GCAGGAAGTT
    ATAAGAGATG TGGAGATAAA TAGAAAGGTC TACAAAGGCA TGCTGGATAT TTTGAAGTGC
    ACGGTGTCCA GTTTGGAGCA TTCATACACT AATGCTGGCT TAGGGGGCAT GGCCAGTGTC
    TTCAGTCTGC TAGAGATAGC ACGCACCCAC TACCAAACCA AAGATCCAGA GAAGCGGAAA
    CGGAGCCCCA CCGAAGGGCT TAGCAGTCCA GGCAGTAAGG AGAGCCCATC GGGCCGAATG
    GAGAGTGCCA GGGCGGCAGG GGTGCTTCTG GTACCCCGTA TCCAATTGCC GCCACCCTCC
    TCTGGGAAAT CATCACGCCA GTTTGATACC CGGAGTCTGA ACGAGGAGAA TTTTATTGCC
    TCCATTGAAT TGTGGAGCAA GCACCAGGAT AACAGAAAGC AAAAAGCTTT GGAAAAAGAA
    CAGAGTGGTT TAAATAAACG GAAATCAGGA GCGGATGGAG TAAAACAACG TGTAGAAGGA
    GGAGACACTG AGGAGAAGAA ATCACAGATC AGTGCAGACA GTGGCCTCAG TGTGACATCT
    GGCTCACAGA AGAGTGATAC GGATTCCCTG GCGAGCTCTG AACCTCCTGC TCTGACCAGA
    AGCACCAGCC AGGACTCAGA GGCCAGCACA GTGGTAAGTA ACAGCTCAGG TGAGACGCTC
    GGTGCAGACA GTGACCTGAG CAGCACTGCT GGAGATGGCC TGACAGGAAG ACATGGTCAG
    CATCTCAACC TGTCCCGTGG CACACTGTCT GACAGTGAGA TTGAGACCAA CCCTGCAACC
    AGCTCAGTGT TTGGCAAGAC ACACAAGCTG AAGCCAGGAG TGAAGGAGCC TGTTGGTGTT
    AATAAAGCAG CACCTGCTCC ACCCGTTGAG GATGTTAGCA TGAGGATCTA TCTGTGTGAG
    GGGCTTCTGG GCCGGGATAA AAGCTCAGTT TGGGACCAGT TGGAGGATGC TGCAATGGAA
    ACCTTTTCTC TGAGTAAAGA GCGCTCAACT CTGTGGGATC AGATGCAGTT CTGGGAGGAC
    GCCTTCCTGG ATGCCGTAAT GCTGGAACGG GAAGGAATGG GAATGGATCA GGGACCACAA
    GAAATGATTG ACAGGTATCT TTCTCTGGGA GATCATGACC GCAAGAGACT GGAGGATGAT
    GAGGACAGAC TGCTGGCCAC GCTTCTGCAC AATATGATTG CTTACATGCT TATGATGAAG
    GTCGGTAAAA ACGACATCAG GAAGAAAGTA CGTCGTCTTA TGGGCAAATC CCACATTGGC
    CTCACTCACA GTCAGGAGAT CAATGAAGTC CTGGATCGCC TCGCACATTT AAGCGGTCGG
    GAGCTTTCTA TCAGGCCGAG TGGCAGCAGG CATATTAAAA AACAGACATT TGTTGTGCAT
    GCTGGGACAG ATACAACTGG TGACATATTC TTCATGGAGG TGTGTGATGA TTGCATCGTC
    TTGCGCAGTA ATATTGGCAC AGTCTATGAG CGCTGGTGGT ATGAGAAGCT CATAAATATG
    ACTTACTGTC CCAAAACTAA AGTGTTGTGT CTGTGGAGGA GAAACGGCCA GGAGACGCAG
    CTAAACAAGT TTTACACTAA GAAGTGTCGG GAATTGTACT ACTGTGTTAA AGACAGCATG
    GAGCGTGCTG CTGCTCGACA ACAAAGCATC AAACCAGGTC CAGAGCTTGG AGGCGAGTTT
    CCGGTTCAGG ACATGAAGAC AGGGGAGGGT GGTCTCCTCC AAGTCACACT GGAAGGCATC
    AACCTCAAAT TTATGCACAG CCAGGAGCGG AAGGTTTTCA TAGAGCTGAA TCACATTAAA
    AAGTGCAATA CAGTGAAAGG AGTCTTTGTC CTGGAGGAGT TTGTTCCTGA AACTAAAGAA
    GTGGTGATCC ATAAGTACAA GACTCCTATG GCACATCAGA TCTGTTACTC GGTGCTCTGC
    CTCTTCTCCT ACATGGCGGC GGTCAAGGGG AAAGAGTCAG AGGGCAAACC CAAGATGCTG
    TCCCCTCGTC CACTAGCGAG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144888
    Clone ID Related Accession (Same CDS sequence) XM_021471958.1
    Accession Version XM_021471958.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5097bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394698.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGGCT
    CTGGCTAGCA TGAGTCTAAA CACACAGCCT ATTCTGAACC TGGAGAAGTT TCAGGATGGA
    CAGGAGTTGT CTTTATTGCC CCCCGGTCGT GACAAAGCTT CCCCGTCCTC CACTGAGTTC
    AACCCACTCA TCTACGGCAA TGATGTGGAC TCAGTTGATG TTGCCACAAG GGTGGCAATG
    GTGAGGTTCT TCAACTCTCC AAACGTGCTG CAAGGGTTCC AGATGCACAC ACGCACTCTC
    CGTCTCTTCC CTCGTCCTGT GGTGGCCTTT CAGTCCACAT CTTTCCTGGC TTCTAGGCCA
    CGACGAAATG GATTCACAGA AAAGCTCTCC CACACTCAGG CAGTGGAGTA CTATGGAGAA
    TGGGCTCTCA ACCCCACCAA CCTGGCTTTC CAAAGGATTC ATAACAATGT GTATGACCCC
    TCTCTAATAG GCGATAAGCC TAAGTGGTAT GCCCACCAGT TACAGCCAGT ATTCTACCGC
    GTGTATGATG GAAACTCTCA TTTAGCGGAG GCCTTGAGTG GACCCCTGCA GGATGAGACC
    AATGATTCAG ACCCAACAGA CGACAGTGGC AGCGACAGTG AAGCATATGA TGACTCCAGC
    TCCTCCTACT CATCTCTTGG AGACTTTGTG AATGAGATGA TCAAAGGGGA CATACAGGGA
    GACACACCAA ATGTTGATCC CCTGACCCAT GCTGCACTGG GAGATGCAGA TGAGGTTGAG
    ATCCATGACT TTCACGAGTA TAAGGGGGAC AGCGGTGAAC CAGAGCCAGA AGGCCCCACA
    GAAGCGGCGG ACAGTCAGCC CTTGCGGTCC AGCTCTAGCA CCACAGCCAG TTCCAGCCCC
    AGTACAGTCA TACAGGGAGT CAACCATGAG CAGAAGGAAC CTGTTGAGGT GGAGCCCATA
    GCAAATGTGA CTTTCCCAAG CTCTGCTCCA GTATTGGGTC CTCCTCCTTT TACAAGACCC
    ACCCCTGATC CAACCCTTGT TGACCCTGCC AATAAGAAGC GAGAATATGA CAACCCCTAT
    TTTGAGCCCC AGTACGGTTT CCCTACAGAG GAAGATGCTG AGACTGATGA GCAGGAGGAG
    AGTTACACAC CACGATTTAA CCAGAATCTC AATGGCAATA AGCCTTCTCG TCCACTGAGA
    CCCAGCAGCC TGAAGCTTCC GGGTGAATCT GACGGAGAGG GAGACTCCAG AAATAGCTCT
    CCTAACTCAA CCATCTCCAA CAATAGCAGT GATGGCTTTG GGGGACTCAT GTCCTTTGCC
    AGTAACTTGT ATAAAAACCA TGGCACCAGT TTCAGTCTGT CCAGCCTGGC TCTGCCTAAT
    AAAGCGAGAG AGAAGAACAC GCCGTTCCCC AGCCTCAAAG AATATTTTAA TTTTGATTTA
    GCAGAGGACA CAGATCAAGA CGATGCTGAT GACAGTCCTG AAAGTCCTGT ATTTGGGCTA
    AATTCTCTAA TGGAGATTGT AACAGAGATC GGCCCGGGGA GCGGAGAAGG TGCCCGAGCC
    CCCAGAGCAC TGGTAGATCA GAAATCCTCT GTTATTAAGC ACAGCCCTAC TGTGAAGAGA
    GAGTCTCCGT CTCCTCAAGG CAGAGCCAAC AATACCAGTG AGAACCAGCA GTTTCTAAAG
    GAGGTGGTCC AGAGCGTGCT GGAGGGGCAG GGTGTGGGCT GGCTTAACAT GAAGAAGGTG
    CGTCGCCTGC TAGAGAATGA ACAGCTCAGA GTCTTTGTGC TCAGCAAACT CAACCGTGCG
    GTCCAGTCAG AGGAGGACGC CCAGCAGGAA GTTATAAGAG ATGTGGAGAT AAATAGAAAG
    GTCTACAAAG GCATGCTGGA TATTTTGAAG TGCACGGTGT CCAGTTTGGA GCATTCATAC
    ACTAATGCTG GCTTAGGGGG CATGGCCAGT GTCTTCAGTC TGCTAGAGAT AGCACGCACC
    CACTACCAAA CCAAAGATCC AGAGAAGCGG AAACGGAGCC CCACCGAAGG GCTTAGCAGT
    CCAGGCAGTA AGGAGAGCCC ATCGGGCCGA ATGGAGAGTG CCAGGGCGGC AGGGGTGCTT
    CTGGTACCCC GTATCCAATT GCCGCCACCC TCCTCTGGGA AATCATCACG CCAGTTTGAT
    ACCCGGAGTC TGAACGAGGA GAATTTTATT GCCTCCATTG AATTGTGGAG CAAGCACCAG
    GATAACAGAA AGCAAAAAGC TTTGGAAAAA GAACAGAGTG GTTTAAATAA ACGGAAATCA
    GGAGCGGATG GAGTAAAACA ACGTGTAGAA GGAGGAGACA CTGAGGAGAA GAAATCACAG
    ATCAGTGCAG ACAGTGGCCT CAGTGTGACA TCTGGCTCAC AGAAGAGTGA TACGGATTCC
    CTGGCGAGCT CTGAACCTCC TGCTCTGACC AGAAGCACCA GCCAGGACTC AGAGGCCAGC
    ACAGTGGTAA GTAACAGCTC AGGTGAGACG CTCGGTGCAG ACAGTGACCT GAGCAGCACT
    GCTGGAGATG GCCTGACAGG AAGACATGGT CAGCATCTCA ACCTGTCCCG TGGCACACTG
    TCTGACAGTG AGATTGAGAC CAACCCTGCA ACCAGCTCAG TGTTTGGCAA GACACACAAG
    CTGAAGCCAG GAGTGAAGGA GCCTGTTGGT GTTAATAAAG CAGCACCTGC TCCACCCGTT
    GAGGATGTTA GCATGAGGAT CTATCTGTGT GAGGGGCTTC TGGGCCGGGA TAAAAGCTCA
    GTTTGGGACC AGTTGGAGGA TGCTGCAATG GAAACCTTTT CTCTGAGTAA AGAGCGCTCA
    ACTCTGTGGG ATCAGATGCA GTTCTGGGAG GACGCCTTCC TGGATGCCGT AATGCTGGAA
    CGGGAAGGAA TGGGAATGGA TCAGGGACCA CAAGAAATGA TTGACAGGTA TCTTTCTCTG
    GGAGATCATG ACCGCAAGAG ACTGGAGGAT GATGAGGACA GACTGCTGGC CACGCTTCTG
    CACAATATGA TTGCTTACAT GCTTATGATG AAGGTCGGTA AAAACGACAT CAGGAAGAAA
    GTACGTCGTC TTATGGGCAA ATCCCACATT GGCCTCACTC ACAGTCAGGA GATCAATGAA
    GTCCTGGATC GCCTCGCACA TTTAAGCGGT CGGGAGCTTT CTATCAGGCC GAGTGGCAGC
    AGGCATATTA AAAAACAGAC ATTTGTTGTG CATGCTGGGA CAGATACAAC TGGTGACATA
    TTCTTCATGG AGGTGTGTGA TGATTGCATC GTCTTGCGCA GTAATATTGG CACAGTCTAT
    GAGCGCTGGT GGTATGAGAA GCTCATAAAT ATGACTTACT GTCCCAAAAC TAAAGTGTTG
    TGTCTGTGGA GGAGAAACGG CCAGGAGACG CAGCTAAACA AGTTTTACAC TAAGAAGTGT
    CGGGAATTGT ACTACTGTGT TAAAGACAGC ATGGAGCGTG CTGCTGCTCG ACAACAAAGC
    ATCAAACCAG GTCCAGAGCT TGGAGGCGAG TTTCCGGTTC AGGACATGAA GACAGGGGAG
    GGTGGTCTCC TCCAAGTCAC ACTGGAAGGC ATCAACCTCA AATTTATGCA CAGCCAGGAG
    CGGAAGGTTT TCATAGAGCT GAATCACATT AAAAAGTGCA ATACAGTGAA AGGAGTCTTT
    GTCCTGGAGG AGTTTGTTCC TGAAACTAAA GAAGTGGTGA TCCATAAGTA CAAGACTCCT
    ATGGCACATC AGATCTGTTA CTCGGTGCTC TGCCTCTTCT CCTACATGGC GGCGGTCAAG
    GGGAAAGAGT CAGAGGGCAA ACCCAAGATG CTGTCCCCTC GTCCACTAGC GAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327633.1
    CDS169..5265
    Translation

    Target ORF information:

    RefSeq Version XM_021471958.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X47, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471958.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGGCT
    CTGGCTAGCA TGAGTCTAAA CACACAGCCT ATTCTGAACC TGGAGAAGTT TCAGGATGGA
    CAGGAGTTGT CTTTATTGCC CCCCGGTCGT GACAAAGCTT CCCCGTCCTC CACTGAGTTC
    AACCCACTCA TCTACGGCAA TGATGTGGAC TCAGTTGATG TTGCCACAAG GGTGGCAATG
    GTGAGGTTCT TCAACTCTCC AAACGTGCTG CAAGGGTTCC AGATGCACAC ACGCACTCTC
    CGTCTCTTCC CTCGTCCTGT GGTGGCCTTT CAGTCCACAT CTTTCCTGGC TTCTAGGCCA
    CGACGAAATG GATTCACAGA AAAGCTCTCC CACACTCAGG CAGTGGAGTA CTATGGAGAA
    TGGGCTCTCA ACCCCACCAA CCTGGCTTTC CAAAGGATTC ATAACAATGT GTATGACCCC
    TCTCTAATAG GCGATAAGCC TAAGTGGTAT GCCCACCAGT TACAGCCAGT ATTCTACCGC
    GTGTATGATG GAAACTCTCA TTTAGCGGAG GCCTTGAGTG GACCCCTGCA GGATGAGACC
    AATGATTCAG ACCCAACAGA CGACAGTGGC AGCGACAGTG AAGCATATGA TGACTCCAGC
    TCCTCCTACT CATCTCTTGG AGACTTTGTG AATGAGATGA TCAAAGGGGA CATACAGGGA
    GACACACCAA ATGTTGATCC CCTGACCCAT GCTGCACTGG GAGATGCAGA TGAGGTTGAG
    ATCCATGACT TTCACGAGTA TAAGGGGGAC AGCGGTGAAC CAGAGCCAGA AGGCCCCACA
    GAAGCGGCGG ACAGTCAGCC CTTGCGGTCC AGCTCTAGCA CCACAGCCAG TTCCAGCCCC
    AGTACAGTCA TACAGGGAGT CAACCATGAG CAGAAGGAAC CTGTTGAGGT GGAGCCCATA
    GCAAATGTGA CTTTCCCAAG CTCTGCTCCA GTATTGGGTC CTCCTCCTTT TACAAGACCC
    ACCCCTGATC CAACCCTTGT TGACCCTGCC AATAAGAAGC GAGAATATGA CAACCCCTAT
    TTTGAGCCCC AGTACGGTTT CCCTACAGAG GAAGATGCTG AGACTGATGA GCAGGAGGAG
    AGTTACACAC CACGATTTAA CCAGAATCTC AATGGCAATA AGCCTTCTCG TCCACTGAGA
    CCCAGCAGCC TGAAGCTTCC GGGTGAATCT GACGGAGAGG GAGACTCCAG AAATAGCTCT
    CCTAACTCAA CCATCTCCAA CAATAGCAGT GATGGCTTTG GGGGACTCAT GTCCTTTGCC
    AGTAACTTGT ATAAAAACCA TGGCACCAGT TTCAGTCTGT CCAGCCTGGC TCTGCCTAAT
    AAAGCGAGAG AGAAGAACAC GCCGTTCCCC AGCCTCAAAG AATATTTTAA TTTTGATTTA
    GCAGAGGACA CAGATCAAGA CGATGCTGAT GACAGTCCTG AAAGTCCTGT ATTTGGGCTA
    AATTCTCTAA TGGAGATTGT AACAGAGATC GGCCCGGGGA GCGGAGAAGG TGCCCGAGCC
    CCCAGAGCAC TGGTAGATCA GAAATCCTCT GTTATTAAGC ACAGCCCTAC TGTGAAGAGA
    GAGTCTCCGT CTCCTCAAGG CAGAGCCAAC AATACCAGTG AGAACCAGCA GTTTCTAAAG
    GAGGTGGTCC AGAGCGTGCT GGAGGGGCAG GGTGTGGGCT GGCTTAACAT GAAGAAGGTG
    CGTCGCCTGC TAGAGAATGA ACAGCTCAGA GTCTTTGTGC TCAGCAAACT CAACCGTGCG
    GTCCAGTCAG AGGAGGACGC CCAGCAGGAA GTTATAAGAG ATGTGGAGAT AAATAGAAAG
    GTCTACAAAG GCATGCTGGA TATTTTGAAG TGCACGGTGT CCAGTTTGGA GCATTCATAC
    ACTAATGCTG GCTTAGGGGG CATGGCCAGT GTCTTCAGTC TGCTAGAGAT AGCACGCACC
    CACTACCAAA CCAAAGATCC AGAGAAGCGG AAACGGAGCC CCACCGAAGG GCTTAGCAGT
    CCAGGCAGTA AGGAGAGCCC ATCGGGCCGA ATGGAGAGTG CCAGGGCGGC AGGGGTGCTT
    CTGGTACCCC GTATCCAATT GCCGCCACCC TCCTCTGGGA AATCATCACG CCAGTTTGAT
    ACCCGGAGTC TGAACGAGGA GAATTTTATT GCCTCCATTG AATTGTGGAG CAAGCACCAG
    GATAACAGAA AGCAAAAAGC TTTGGAAAAA GAACAGAGTG GTTTAAATAA ACGGAAATCA
    GGAGCGGATG GAGTAAAACA ACGTGTAGAA GGAGGAGACA CTGAGGAGAA GAAATCACAG
    ATCAGTGCAG ACAGTGGCCT CAGTGTGACA TCTGGCTCAC AGAAGAGTGA TACGGATTCC
    CTGGCGAGCT CTGAACCTCC TGCTCTGACC AGAAGCACCA GCCAGGACTC AGAGGCCAGC
    ACAGTGGTAA GTAACAGCTC AGGTGAGACG CTCGGTGCAG ACAGTGACCT GAGCAGCACT
    GCTGGAGATG GCCTGACAGG AAGACATGGT CAGCATCTCA ACCTGTCCCG TGGCACACTG
    TCTGACAGTG AGATTGAGAC CAACCCTGCA ACCAGCTCAG TGTTTGGCAA GACACACAAG
    CTGAAGCCAG GAGTGAAGGA GCCTGTTGGT GTTAATAAAG CAGCACCTGC TCCACCCGTT
    GAGGATGTTA GCATGAGGAT CTATCTGTGT GAGGGGCTTC TGGGCCGGGA TAAAAGCTCA
    GTTTGGGACC AGTTGGAGGA TGCTGCAATG GAAACCTTTT CTCTGAGTAA AGAGCGCTCA
    ACTCTGTGGG ATCAGATGCA GTTCTGGGAG GACGCCTTCC TGGATGCCGT AATGCTGGAA
    CGGGAAGGAA TGGGAATGGA TCAGGGACCA CAAGAAATGA TTGACAGGTA TCTTTCTCTG
    GGAGATCATG ACCGCAAGAG ACTGGAGGAT GATGAGGACA GACTGCTGGC CACGCTTCTG
    CACAATATGA TTGCTTACAT GCTTATGATG AAGGTCGGTA AAAACGACAT CAGGAAGAAA
    GTACGTCGTC TTATGGGCAA ATCCCACATT GGCCTCACTC ACAGTCAGGA GATCAATGAA
    GTCCTGGATC GCCTCGCACA TTTAAGCGGT CGGGAGCTTT CTATCAGGCC GAGTGGCAGC
    AGGCATATTA AAAAACAGAC ATTTGTTGTG CATGCTGGGA CAGATACAAC TGGTGACATA
    TTCTTCATGG AGGTGTGTGA TGATTGCATC GTCTTGCGCA GTAATATTGG CACAGTCTAT
    GAGCGCTGGT GGTATGAGAA GCTCATAAAT ATGACTTACT GTCCCAAAAC TAAAGTGTTG
    TGTCTGTGGA GGAGAAACGG CCAGGAGACG CAGCTAAACA AGTTTTACAC TAAGAAGTGT
    CGGGAATTGT ACTACTGTGT TAAAGACAGC ATGGAGCGTG CTGCTGCTCG ACAACAAAGC
    ATCAAACCAG GTCCAGAGCT TGGAGGCGAG TTTCCGGTTC AGGACATGAA GACAGGGGAG
    GGTGGTCTCC TCCAAGTCAC ACTGGAAGGC ATCAACCTCA AATTTATGCA CAGCCAGGAG
    CGGAAGGTTT TCATAGAGCT GAATCACATT AAAAAGTGCA ATACAGTGAA AGGAGTCTTT
    GTCCTGGAGG AGTTTGTTCC TGAAACTAAA GAAGTGGTGA TCCATAAGTA CAAGACTCCT
    ATGGCACATC AGATCTGTTA CTCGGTGCTC TGCCTCTTCT CCTACATGGC GGCGGTCAAG
    GGGAAAGAGT CAGAGGGCAA ACCCAAGATG CTGTCCCCTC GTCCACTAGC GAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144889
    Clone ID Related Accession (Same CDS sequence) XM_021471959.1
    Accession Version XM_021471959.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5094bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394698.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA CTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGGACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCGGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT TCCCAAGCTC TGCTCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTCCCCAGC CTCAAAGAAT ATTTTAATTT TGATTTAGCA
    GAGGACACAG ATCAAGACGA TGCTGATGAC AGTCCTGAAA GTCCTGGTGC CCGAGCCCCC
    AGAGCACTGG TAGATCAGAA ATCCTCTGTT ATTAAGCACA GCCCTACTGT GAAGAGAGAG
    TCTCCGTCTC CTCAAGGCAG AGCCAACAAT ACCAGTGAGA ACCAGCAGTT TCTAAAGGAG
    GTGGTCCAGA GCGTGCTGGA GGGGCAGGGT GTGGGCTGGC TTAACATGAA GAAGGTGCGT
    CGCCTGCTAG AGAATGAACA GCTCAGAGTC TTTGTGCTCA GCAAACTCAA CCGTGCGGTC
    CAGTCAGAGG AGGACGCCCA GCAGGAAGTT ATAAGAGATG TGGAGATAAA TAGAAAGGTC
    TACAAAGGCA TGCTGGATAT TTTGAAGTGC ACGGTGTCCA GTTTGGAGCA TTCATACACT
    AATGCTGGCT TAGGGGGCAT GGCCAGTGTC TTCAGTCTGC TAGAGATAGC ACGCACCCAC
    TACCAAACCA AAGATCCAGA GAAGCGGAAA CGGAGCCCCA CCGAAGGGCT TAGCAGTCCA
    GGCAGTAAGG AGAGCCCATC GGGCCGAATG GAGAGTGCCA GGGCGGCAGG GGTGCTTCTG
    GTACCCCGTA TCCAATTGCC GCCACCCTCC TCTGGGAAAT CATCACGCCA GTTTGATACC
    CGGAGTCTGA ACGAGGAGAA TTTTATTGCC TCCATTGAAT TGTGGAGCAA GCACCAGGAT
    AACAGAAAGC AAAAAGCTTT GGAAAAAGAA CAGAGTGGTT TAAATAAACG GAAATCAGGA
    GCGGATGGAG TAAAACAACG TGTAGAAGGA GGAGACACTG AGGAGAAGAA ATCACAGATC
    AGTGCAGACA GTGGCCTCAG TGTGACATCT GGCTCACAGA AGAGTGATAC GGATTCCCTG
    GCGAGCTCTG AACCTCCTGC TCTGACCAGA AGCACCAGCC AGGACTCAGA GGCCAGCACA
    GTGGTAAGTA ACAGCTCAGG TGAGACGCTC GGTGCAGACA GTGACCTGAG CAGCACTGCT
    GGAGATGGCC TGACAGGAAG ACATGGTCAG CATCTCAACC TGTCCCGTGG CACACTGTCT
    GACAGTGAGA TTGAGACCAA CCCTGCAACC AGCTCAGTGT TTGGCAAGAC ACACAAGCTG
    AAGCCAGGAG TGAAGGAGCC TGTTGGTGTT AATAAAGCAG CACCTGCTCC ACCCGTTGAG
    GATGTTAGCA TGAGGATCTA TCTGTGTGAG GGGCTTCTGG GCCGGGATAA AAGCTCAGTT
    TGGGACCAGT TGGAGGATGC TGCAATGGAA ACCTTTTCTC TGAGTAAAGA GCGCTCAACT
    CTGTGGGATC AGATGCAGTT CTGGGAGGAC GCCTTCCTGG ATGCCGTAAT GCTGGAACGG
    GAAGGAATGG GAATGGATCA GGGACCACAA GAAATGATTG ACAGGTATCT TTCTCTGGGA
    GATCATGACC GCAAGAGACT GGAGGATGAT GAGGACAGAC TGCTGGCCAC GCTTCTGCAC
    AATATGATTG CTTACATGCT TATGATGAAG GTCGGTAAAA ACGACATCAG GAAGAAAGTA
    CGTCGTCTTA TGGGCAAATC CCACATTGGC CTCACTCACA GTCAGGAGAT CAATGAAGTC
    CTGGATCGCC TCGCACATTT AAGCGGTCGG GAGCTTTCTA TCAGGCCGAG TGGCAGCAGG
    CATATTAAAA AACAGACATT TGTTGTGCAT GCTGGGACAG ATACAACTGG TGACATATTC
    TTCATGGAGG TGTGTGATGA TTGCATCGTC TTGCGCAGTA ATATTGGCAC AGTCTATGAG
    CGCTGGTGGT ATGAGAAGCT CATAAATATG ACTTACTGTC CCAAAACTAA AGTGTTGTGT
    CTGTGGAGGA GAAACGGCCA GGAGACGCAG CTAAACAAGT TTTACACTAA GAAGTGTCGG
    GAATTGTACT ACTGTGTTAA AGACAGCATG GAGCGTGCTG CTGCTCGACA ACAAAGCATC
    AAACCAGGTC CAGAGCTTGG AGGCGAGTTT CCGGTTCAGG ACATGAAGAC AGGGGAGGGT
    GGTCTCCTCC AAGTCACACT GGAAGGCATC AACCTCAAAT TTATGCACAG CCAGGAGCGG
    AAGGTTTTCA TAGAGCTGAA TCACATTAAA AAGTGCAATA CAGTGAAAGG AGTCTTTGTC
    CTGGAGGAGT TTGTTCCTGA AACTAAAGAA GTGGTGATCC ATAAGTACAA GACTCCTATG
    GCACATCAGA TCTGTTACTC GGTGCTCTGC CTCTTCTCCT ACATGGCGGC GGTCAAGGGG
    AAAGAGTCAG AGGGCAAACC CAAGATGCTG TCCCCTCGTC CACTAGCGAG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327634.1
    CDS169..5262
    Translation

    Target ORF information:

    RefSeq Version XM_021471959.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X48, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471959.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA CTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGGACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCGGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT TCCCAAGCTC TGCTCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTCCCCAGC CTCAAAGAAT ATTTTAATTT TGATTTAGCA
    GAGGACACAG ATCAAGACGA TGCTGATGAC AGTCCTGAAA GTCCTGGTGC CCGAGCCCCC
    AGAGCACTGG TAGATCAGAA ATCCTCTGTT ATTAAGCACA GCCCTACTGT GAAGAGAGAG
    TCTCCGTCTC CTCAAGGCAG AGCCAACAAT ACCAGTGAGA ACCAGCAGTT TCTAAAGGAG
    GTGGTCCAGA GCGTGCTGGA GGGGCAGGGT GTGGGCTGGC TTAACATGAA GAAGGTGCGT
    CGCCTGCTAG AGAATGAACA GCTCAGAGTC TTTGTGCTCA GCAAACTCAA CCGTGCGGTC
    CAGTCAGAGG AGGACGCCCA GCAGGAAGTT ATAAGAGATG TGGAGATAAA TAGAAAGGTC
    TACAAAGGCA TGCTGGATAT TTTGAAGTGC ACGGTGTCCA GTTTGGAGCA TTCATACACT
    AATGCTGGCT TAGGGGGCAT GGCCAGTGTC TTCAGTCTGC TAGAGATAGC ACGCACCCAC
    TACCAAACCA AAGATCCAGA GAAGCGGAAA CGGAGCCCCA CCGAAGGGCT TAGCAGTCCA
    GGCAGTAAGG AGAGCCCATC GGGCCGAATG GAGAGTGCCA GGGCGGCAGG GGTGCTTCTG
    GTACCCCGTA TCCAATTGCC GCCACCCTCC TCTGGGAAAT CATCACGCCA GTTTGATACC
    CGGAGTCTGA ACGAGGAGAA TTTTATTGCC TCCATTGAAT TGTGGAGCAA GCACCAGGAT
    AACAGAAAGC AAAAAGCTTT GGAAAAAGAA CAGAGTGGTT TAAATAAACG GAAATCAGGA
    GCGGATGGAG TAAAACAACG TGTAGAAGGA GGAGACACTG AGGAGAAGAA ATCACAGATC
    AGTGCAGACA GTGGCCTCAG TGTGACATCT GGCTCACAGA AGAGTGATAC GGATTCCCTG
    GCGAGCTCTG AACCTCCTGC TCTGACCAGA AGCACCAGCC AGGACTCAGA GGCCAGCACA
    GTGGTAAGTA ACAGCTCAGG TGAGACGCTC GGTGCAGACA GTGACCTGAG CAGCACTGCT
    GGAGATGGCC TGACAGGAAG ACATGGTCAG CATCTCAACC TGTCCCGTGG CACACTGTCT
    GACAGTGAGA TTGAGACCAA CCCTGCAACC AGCTCAGTGT TTGGCAAGAC ACACAAGCTG
    AAGCCAGGAG TGAAGGAGCC TGTTGGTGTT AATAAAGCAG CACCTGCTCC ACCCGTTGAG
    GATGTTAGCA TGAGGATCTA TCTGTGTGAG GGGCTTCTGG GCCGGGATAA AAGCTCAGTT
    TGGGACCAGT TGGAGGATGC TGCAATGGAA ACCTTTTCTC TGAGTAAAGA GCGCTCAACT
    CTGTGGGATC AGATGCAGTT CTGGGAGGAC GCCTTCCTGG ATGCCGTAAT GCTGGAACGG
    GAAGGAATGG GAATGGATCA GGGACCACAA GAAATGATTG ACAGGTATCT TTCTCTGGGA
    GATCATGACC GCAAGAGACT GGAGGATGAT GAGGACAGAC TGCTGGCCAC GCTTCTGCAC
    AATATGATTG CTTACATGCT TATGATGAAG GTCGGTAAAA ACGACATCAG GAAGAAAGTA
    CGTCGTCTTA TGGGCAAATC CCACATTGGC CTCACTCACA GTCAGGAGAT CAATGAAGTC
    CTGGATCGCC TCGCACATTT AAGCGGTCGG GAGCTTTCTA TCAGGCCGAG TGGCAGCAGG
    CATATTAAAA AACAGACATT TGTTGTGCAT GCTGGGACAG ATACAACTGG TGACATATTC
    TTCATGGAGG TGTGTGATGA TTGCATCGTC TTGCGCAGTA ATATTGGCAC AGTCTATGAG
    CGCTGGTGGT ATGAGAAGCT CATAAATATG ACTTACTGTC CCAAAACTAA AGTGTTGTGT
    CTGTGGAGGA GAAACGGCCA GGAGACGCAG CTAAACAAGT TTTACACTAA GAAGTGTCGG
    GAATTGTACT ACTGTGTTAA AGACAGCATG GAGCGTGCTG CTGCTCGACA ACAAAGCATC
    AAACCAGGTC CAGAGCTTGG AGGCGAGTTT CCGGTTCAGG ACATGAAGAC AGGGGAGGGT
    GGTCTCCTCC AAGTCACACT GGAAGGCATC AACCTCAAAT TTATGCACAG CCAGGAGCGG
    AAGGTTTTCA TAGAGCTGAA TCACATTAAA AAGTGCAATA CAGTGAAAGG AGTCTTTGTC
    CTGGAGGAGT TTGTTCCTGA AACTAAAGAA GTGGTGATCC ATAAGTACAA GACTCCTATG
    GCACATCAGA TCTGTTACTC GGTGCTCTGC CTCTTCTCCT ACATGGCGGC GGTCAAGGGG
    AAAGAGTCAG AGGGCAAACC CAAGATGCTG TCCCCTCGTC CACTAGCGAG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144891
    Clone ID Related Accession (Same CDS sequence) XM_021471961.1
    Accession Version XM_021471961.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5088bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394698.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGACTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGGA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCGGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTTCCCAA GCTCTGCTCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTCCC CAGCCTCAAA GAATATTTTA ATTTTGATTT AGCAGAGGAC
    ACAGATCAAG ACGATGCTGA TGACAGTCCT GAAAGTCCTG TATTTGGGCT AAATTCTCTA
    ATGGAGATTG TAACAGAGAT CGGCCCGGGG AGCGGAGAAG GTGCCCGAGC CCCCAGAGCA
    CTGGTAGATC AGAAATCCTC TGTTATTAAG CACAGCCCTA CTGTGAAGAG AGAGTCTCCG
    TCTCCTCAAG GCAGAGCCAA CAATACCAGT GAGAACCAGC AGTTTCTAAA GGAGGTGGTC
    CAGAGCGTGC TGGAGGGGCA GGGTGTGGGC TGGCTTAACA TGAAGAAGGT GCGTCGCCTG
    CTAGAGAATG AACAGCTCAG AGTCTTTGTG CTCAGCAAAC TCAACCGTGC GGTCCAGTCA
    GAGGAGGACG CCCAGCAGGA AGTTATAAGA GATGTGGAGA TAAATAGAAA GGTCTACAAA
    GGCATGCTGG ATATTTTGAA GTGCACGGTG TCCAGTTTGG AGCATTCATA CACTAATGCT
    GGCTTAGGGG GCATGGCCAG TGTCTTCAGT CTGCTAGAGA TAGCACGCAC CCACTACCAA
    ACCAAAGATC CAGAGAAGCG GAAACGGAGC CCCACCGAAG GGCTTAGCAG TCCAGGCAGT
    AAGGAGAGCC CATCGGGCCG AATGGAGAGT GCCAGGGCGG CAGGGGTGCT TCTGGTACCC
    CGTATCCAAT TGCCGCCACC CTCCTCTGGG AAATCATCAC GCCAGTTTGA TACCCGGAGT
    CTGAACGAGG AGAATTTTAT TGCCTCCATT GAATTGTGGA GCAAGCACCA GGATAACAGA
    AAGCAAAAAG CTTTGGAAAA AGAACAGAGT GGTTTAAATA AACGGAAATC AGGAGCGGAT
    GGAGTAAAAC AACGTGTAGA AGGAGGAGAC ACTGAGGAGA AGAAATCACA GATCAGTGCA
    GACAGTGGCC TCAGTGTGAC ATCTGGCTCA CAGAAGAGTG ATACGGATTC CCTGGCGAGC
    TCTGAACCTC CTGCTCTGAC CAGAAGCACC AGCCAGGACT CAGAGGCCAG CACAGTGGTA
    AGTAACAGCT CAGGTGAGAC GCTCGGTGCA GACAGTGACC TGAGCAGCAC TGCTGGAGAT
    GGCCTGACAG GAAGACATGG TCAGCATCTC AACCTGTCCC GTGGCACACT GTCTGACAGT
    GAGATTGAGA CCAACCCTGC AACCAGCTCA GTGTTTGGCA AGACACACAA GCTGAAGCCA
    GGAGTGAAGG AGCCTGTTGG TGTTAATAAA GCAGCACCTG CTCCACCCGT TGAGGATGTT
    AGCATGAGGA TCTATCTGTG TGAGGGGCTT CTGGGCCGGG ATAAAAGCTC AGTTTGGGAC
    CAGTTGGAGG ATGCTGCAAT GGAAACCTTT TCTCTGAGTA AAGAGCGCTC AACTCTGTGG
    GATCAGATGC AGTTCTGGGA GGACGCCTTC CTGGATGCCG TAATGCTGGA ACGGGAAGGA
    ATGGGAATGG ATCAGGGACC ACAAGAAATG ATTGACAGGT ATCTTTCTCT GGGAGATCAT
    GACCGCAAGA GACTGGAGGA TGATGAGGAC AGACTGCTGG CCACGCTTCT GCACAATATG
    ATTGCTTACA TGCTTATGAT GAAGGTCGGT AAAAACGACA TCAGGAAGAA AGTACGTCGT
    CTTATGGGCA AATCCCACAT TGGCCTCACT CACAGTCAGG AGATCAATGA AGTCCTGGAT
    CGCCTCGCAC ATTTAAGCGG TCGGGAGCTT TCTATCAGGC CGAGTGGCAG CAGGCATATT
    AAAAAACAGA CATTTGTTGT GCATGCTGGG ACAGATACAA CTGGTGACAT ATTCTTCATG
    GAGGTGTGTG ATGATTGCAT CGTCTTGCGC AGTAATATTG GCACAGTCTA TGAGCGCTGG
    TGGTATGAGA AGCTCATAAA TATGACTTAC TGTCCCAAAA CTAAAGTGTT GTGTCTGTGG
    AGGAGAAACG GCCAGGAGAC GCAGCTAAAC AAGTTTTACA CTAAGAAGTG TCGGGAATTG
    TACTACTGTG TTAAAGACAG CATGGAGCGT GCTGCTGCTC GACAACAAAG CATCAAACCA
    GGTCCAGAGC TTGGAGGCGA GTTTCCGGTT CAGGACATGA AGACAGGGGA GGGTGGTCTC
    CTCCAAGTCA CACTGGAAGG CATCAACCTC AAATTTATGC ACAGCCAGGA GCGGAAGGTT
    TTCATAGAGC TGAATCACAT TAAAAAGTGC AATACAGTGA AAGGAGTCTT TGTCCTGGAG
    GAGTTTGTTC CTGAAACTAA AGAAGTGGTG ATCCATAAGT ACAAGACTCC TATGGCACAT
    CAGATCTGTT ACTCGGTGCT CTGCCTCTTC TCCTACATGG CGGCGGTCAA GGGGAAAGAG
    TCAGAGGGCA AACCCAAGAT GCTGTCCCCT CGTCCACTAG CGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327636.1
    CDS169..5256
    Translation

    Target ORF information:

    RefSeq Version XM_021471961.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X50, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471961.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGACTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGGA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCGGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTTCCCAA GCTCTGCTCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTCCC CAGCCTCAAA GAATATTTTA ATTTTGATTT AGCAGAGGAC
    ACAGATCAAG ACGATGCTGA TGACAGTCCT GAAAGTCCTG TATTTGGGCT AAATTCTCTA
    ATGGAGATTG TAACAGAGAT CGGCCCGGGG AGCGGAGAAG GTGCCCGAGC CCCCAGAGCA
    CTGGTAGATC AGAAATCCTC TGTTATTAAG CACAGCCCTA CTGTGAAGAG AGAGTCTCCG
    TCTCCTCAAG GCAGAGCCAA CAATACCAGT GAGAACCAGC AGTTTCTAAA GGAGGTGGTC
    CAGAGCGTGC TGGAGGGGCA GGGTGTGGGC TGGCTTAACA TGAAGAAGGT GCGTCGCCTG
    CTAGAGAATG AACAGCTCAG AGTCTTTGTG CTCAGCAAAC TCAACCGTGC GGTCCAGTCA
    GAGGAGGACG CCCAGCAGGA AGTTATAAGA GATGTGGAGA TAAATAGAAA GGTCTACAAA
    GGCATGCTGG ATATTTTGAA GTGCACGGTG TCCAGTTTGG AGCATTCATA CACTAATGCT
    GGCTTAGGGG GCATGGCCAG TGTCTTCAGT CTGCTAGAGA TAGCACGCAC CCACTACCAA
    ACCAAAGATC CAGAGAAGCG GAAACGGAGC CCCACCGAAG GGCTTAGCAG TCCAGGCAGT
    AAGGAGAGCC CATCGGGCCG AATGGAGAGT GCCAGGGCGG CAGGGGTGCT TCTGGTACCC
    CGTATCCAAT TGCCGCCACC CTCCTCTGGG AAATCATCAC GCCAGTTTGA TACCCGGAGT
    CTGAACGAGG AGAATTTTAT TGCCTCCATT GAATTGTGGA GCAAGCACCA GGATAACAGA
    AAGCAAAAAG CTTTGGAAAA AGAACAGAGT GGTTTAAATA AACGGAAATC AGGAGCGGAT
    GGAGTAAAAC AACGTGTAGA AGGAGGAGAC ACTGAGGAGA AGAAATCACA GATCAGTGCA
    GACAGTGGCC TCAGTGTGAC ATCTGGCTCA CAGAAGAGTG ATACGGATTC CCTGGCGAGC
    TCTGAACCTC CTGCTCTGAC CAGAAGCACC AGCCAGGACT CAGAGGCCAG CACAGTGGTA
    AGTAACAGCT CAGGTGAGAC GCTCGGTGCA GACAGTGACC TGAGCAGCAC TGCTGGAGAT
    GGCCTGACAG GAAGACATGG TCAGCATCTC AACCTGTCCC GTGGCACACT GTCTGACAGT
    GAGATTGAGA CCAACCCTGC AACCAGCTCA GTGTTTGGCA AGACACACAA GCTGAAGCCA
    GGAGTGAAGG AGCCTGTTGG TGTTAATAAA GCAGCACCTG CTCCACCCGT TGAGGATGTT
    AGCATGAGGA TCTATCTGTG TGAGGGGCTT CTGGGCCGGG ATAAAAGCTC AGTTTGGGAC
    CAGTTGGAGG ATGCTGCAAT GGAAACCTTT TCTCTGAGTA AAGAGCGCTC AACTCTGTGG
    GATCAGATGC AGTTCTGGGA GGACGCCTTC CTGGATGCCG TAATGCTGGA ACGGGAAGGA
    ATGGGAATGG ATCAGGGACC ACAAGAAATG ATTGACAGGT ATCTTTCTCT GGGAGATCAT
    GACCGCAAGA GACTGGAGGA TGATGAGGAC AGACTGCTGG CCACGCTTCT GCACAATATG
    ATTGCTTACA TGCTTATGAT GAAGGTCGGT AAAAACGACA TCAGGAAGAA AGTACGTCGT
    CTTATGGGCA AATCCCACAT TGGCCTCACT CACAGTCAGG AGATCAATGA AGTCCTGGAT
    CGCCTCGCAC ATTTAAGCGG TCGGGAGCTT TCTATCAGGC CGAGTGGCAG CAGGCATATT
    AAAAAACAGA CATTTGTTGT GCATGCTGGG ACAGATACAA CTGGTGACAT ATTCTTCATG
    GAGGTGTGTG ATGATTGCAT CGTCTTGCGC AGTAATATTG GCACAGTCTA TGAGCGCTGG
    TGGTATGAGA AGCTCATAAA TATGACTTAC TGTCCCAAAA CTAAAGTGTT GTGTCTGTGG
    AGGAGAAACG GCCAGGAGAC GCAGCTAAAC AAGTTTTACA CTAAGAAGTG TCGGGAATTG
    TACTACTGTG TTAAAGACAG CATGGAGCGT GCTGCTGCTC GACAACAAAG CATCAAACCA
    GGTCCAGAGC TTGGAGGCGA GTTTCCGGTT CAGGACATGA AGACAGGGGA GGGTGGTCTC
    CTCCAAGTCA CACTGGAAGG CATCAACCTC AAATTTATGC ACAGCCAGGA GCGGAAGGTT
    TTCATAGAGC TGAATCACAT TAAAAAGTGC AATACAGTGA AAGGAGTCTT TGTCCTGGAG
    GAGTTTGTTC CTGAAACTAA AGAAGTGGTG ATCCATAAGT ACAAGACTCC TATGGCACAT
    CAGATCTGTT ACTCGGTGCT CTGCCTCTTC TCCTACATGG CGGCGGTCAA GGGGAAAGAG
    TCAGAGGGCA AACCCAAGAT GCTGTCCCCT CGTCCACTAG CGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144894
    Clone ID Related Accession (Same CDS sequence) XM_021471964.1
    Accession Version XM_021471964.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5079bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394698.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGGG AACATTCGGC GCCCCCTGCA
    GGAGATGGGA CTTTGCTGCC AGGCGAGCCG GGTAGCAGCG GGAAGTCTCC GCGATCTAAA
    CGCAGTGGCC GAATAGCACC ACAGAACCGC AACAGCATGT TAACATCTCT GTGTATTCTG
    AGCCACTACC CTTTCTTCTC AACATTCAGA GAGTGTCTGT ACATCCTCAA GCGCATGGTG
    GACTGCTGTA GTCAGCGGCT CAACCAGAGA CCCGGAGCTC CCAAATCCAC ACAACGGGAC
    ACGATGTGGC GTGTGTTCAC GGGAGCTCTT TCTGTGGAAG AAAAGGAGAA GGGCAGTCAG
    GTGCTCCAGG ACCTGAGGGA GATCGAGTCA TGGGTTTATC GGCTGCTGCG CTCACCGGTG
    CCTGTTGCAG GTCAGCGGCG TGTGGATGTG GAGGTCCTGC CCCATGAACT GCAGCCTGCT
    CTGACATTTG CCCTTCCAGA CCCGTCCCGC TTCAGCATCG TAGACTTCCC GCTCCACCTG
    CCACTGGAGC TGCTGGGAGT CGATGCCTGT CTGCAGGTTT TGGCCTGCAT TCTGCTGGAG
    CACAAGGTGG TACTGCAGTC TCGAGATTAT AACGCTCTCT CAATGAGCGT CATGGCGTTT
    GTTTCGATGA TTTACCCTCT TGAGTACATG TTTCCTGTCA TTCCACTGTT GCCCACCTGC
    ATGGCCTCTG CAGAACAGCT CCTATTGGCA CCCACTCCAT ACGTCATTGG TGTGCCAGCC
    AGTTTCTTCC TGTACAAGTG TGATTTTAAG ATGCCTGATG ATGTTTGGTT AGTGGATCTG
    GACTGCAACA AGGTTATTGT TCCAAGCAAT GCAGAACTGC TCCCACCCTT ACCTGAACCT
    GAGGCCTCTG AGCTGAAGAA ACACTTGAAG CAGCTCTTGG AGTGTCTGGT AAGGTTGACT
    CTGATCACCC AAAAGCAGAT CTTCGCCTCC GATTCTAAGG CTCTGGCTAG CATGAGTCTA
    AACACACAGC CTATTCTGAA CCTGGAGAAG TTTCAGGATG GACAGGAGTT GTCTTTATTG
    CCCCCCGGTC GTGACAAAGC TTCCCCGTCC TCCACTGAGT TCAACCCACT CATCTACGGC
    AATGATGTGG ACTCAGTTGA TGTTGCCACA AGGGTGGCAA TGGTGAGGTT CTTCAACTCT
    CCAAACGTGC TGCAAGGGTT CCAGATGCAC ACACGCACTC TCCGTCTCTT CCCTCGTCCT
    GTGGTGGCCT TTCAGTCCAC ATCTTTCCTG GCTTCTAGGC CACGACGAAA TGGATTCACA
    GAAAAGCTCT CCCACACTCA GGCAGTGGAG TACTATGGAG AATGGGCTCT CAACCCCACC
    AACCTGGCTT TCCAAAGGAT TCATAACAAT GTGTATGACC CCTCTCTAAT AGGCGATAAG
    CCTAAGTGGT ATGCCCACCA GTTACAGCCA GTATTCTACC GCGTGTATGA TGGAAACTCT
    CATTTAGCGG AGGCCTTGAG TGGACCCCTG CAGGATGAGA CCAATGATTC AGACCCAACA
    GACGACAGTG GCAGCGACAG TGAAGCATAT GATGACTCCA GCTCCTCCTA CTCATCTCTT
    GGAGACTTTG TGAATGAGAT GATCAAAGGG GACATACAGG GAGACACACC AAATGTTGAT
    CCCCTGACCC ATGCTGCACT GGGAGATGCA GATGAGGTTG AGATCCATGA CTTTCACGAG
    TATAAGGGGG ACAGCGGTGA ACCAGAGCCA GAAGGCCCCA CAGAAGCGGC GGACAGTCAG
    CCCTTGCGGT CCAGCTCTAG CACCACAGCC AGTTCCAGCC CCAGTACAGT CATACAGGGA
    GTCAACCATG AGCAGAAGGA ACCTGTTGAG GTGGAGCCCA TAGCAAATGT GACTTTCCCA
    AGCTCTGCTC CAGTATTGGG TCCTCCTCCT TTTACAAGAC CCACCCCTGA TCCAACCCTT
    GTTGACCCTG CCAATAAGAA GCGAGAATAT GACAACCCCT ATTTTGAGCC CCAGTACGGT
    TTCCCTACAG AGGAAGATGC TGAGACTGAT GAGCAGGAGG AGAGTTACAC ACCACGATTT
    AACCAGAATC TCAATGGCAA TAAGCCTTCT CGTCCACTGA GACCCAGCAG CCTGAAGCTT
    CCGGGTGAAT CTGACGGAGA GGGAGACTCC AGAAATAGCT CTCCTAACTC AACCATCTCC
    AACAATAGCA GTGATGGCTT TGGGGGACTC ATGTCCTTTG CCAGTAACTT GTATAAAAAC
    CATGGCACCA GTTTCAGTCT GTCCAGCCTG GCTCTGCCTA ATAAAGCGAG AGAGAAGAAC
    ACGCCGTTCC CCAGCCTCAA AGAATATTTT AATTTTGATT TAGCAGAGGA CACAGATCAA
    GACGATGCTG ATGACAGTCC TGAAAGTCCT GTATTTGGGC TAAATTCTCT AATGGAGATT
    GTAACAGAGA TCGGCCCGGG GAGCGGAGAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGAATTGTGG AGCAAGCACC AGGATAACAG AAAGCAAAAA
    GCTTTGGAAA AAGAACAGAG TGGTTTAAAT AAACGGAAAT CAGGAGCGGA TGGAGTAAAA
    CAACGTGTAG AAGGAGGAGA CACTGAGGAG AAGAAATCAC AGATCAGTGC AGACAGTGGC
    CTCAGTGTGA CATCTGGCTC ACAGAAGAGT GATACGGATT CCCTGGCGAG CTCTGAACCT
    CCTGCTCTGA CCAGAAGCAC CAGCCAGGAC TCAGAGGCCA GCACAGTGGT AAGTAACAGC
    TCAGGTGAGA CGCTCGGTGC AGACAGTGAC CTGAGCAGCA CTGCTGGAGA TGGCCTGACA
    GGAAGACATG GTCAGCATCT CAACCTGTCC CGTGGCACAC TGTCTGACAG TGAGATTGAG
    ACCAACCCTG CAACCAGCTC AGTGTTTGGC AAGACACACA AGCTGAAGCC AGGAGTGAAG
    GAGCCTGTTG GTGTTAATAA AGCAGCACCT GCTCCACCCG TTGAGGATGT TAGCATGAGG
    ATCTATCTGT GTGAGGGGCT TCTGGGCCGG GATAAAAGCT CAGTTTGGGA CCAGTTGGAG
    GATGCTGCAA TGGAAACCTT TTCTCTGAGT AAAGAGCGCT CAACTCTGTG GGATCAGATG
    CAGTTCTGGG AGGACGCCTT CCTGGATGCC GTAATGCTGG AACGGGAAGG AATGGGAATG
    GATCAGGGAC CACAAGAAAT GATTGACAGG TATCTTTCTC TGGGAGATCA TGACCGCAAG
    AGACTGGAGG ATGATGAGGA CAGACTGCTG GCCACGCTTC TGCACAATAT GATTGCTTAC
    ATGCTTATGA TGAAGGTCGG TAAAAACGAC ATCAGGAAGA AAGTACGTCG TCTTATGGGC
    AAATCCCACA TTGGCCTCAC TCACAGTCAG GAGATCAATG AAGTCCTGGA TCGCCTCGCA
    CATTTAAGCG GTCGGGAGCT TTCTATCAGG CCGAGTGGCA GCAGGCATAT TAAAAAACAG
    ACATTTGTTG TGCATGCTGG GACAGATACA ACTGGTGACA TATTCTTCAT GGAGGTGTGT
    GATGATTGCA TCGTCTTGCG CAGTAATATT GGCACAGTCT ATGAGCGCTG GTGGTATGAG
    AAGCTCATAA ATATGACTTA CTGTCCCAAA ACTAAAGTGT TGTGTCTGTG GAGGAGAAAC
    GGCCAGGAGA CGCAGCTAAA CAAGTTTTAC ACTAAGAAGT GTCGGGAATT GTACTACTGT
    GTTAAAGACA GCATGGAGCG TGCTGCTGCT CGACAACAAA GCATCAAACC AGGTCCAGAG
    CTTGGAGGCG AGTTTCCGGT TCAGGACATG AAGACAGGGG AGGGTGGTCT CCTCCAAGTC
    ACACTGGAAG GCATCAACCT CAAATTTATG CACAGCCAGG AGCGGAAGGT TTTCATAGAG
    CTGAATCACA TTAAAAAGTG CAATACAGTG AAAGGAGTCT TTGTCCTGGA GGAGTTTGTT
    CCTGAAACTA AAGAAGTGGT GATCCATAAG TACAAGACTC CTATGGCACA TCAGATCTGT
    TACTCGGTGC TCTGCCTCTT CTCCTACATG GCGGCGGTCA AGGGGAAAGA GTCAGAGGGC
    AAACCCAAGA TGCTGTCCCC TCGTCCACTA GCGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327639.1
    CDS169..5247
    Translation

    Target ORF information:

    RefSeq Version XM_021471964.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X53, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471964.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGGG AACATTCGGC GCCCCCTGCA
    GGAGATGGGA CTTTGCTGCC AGGCGAGCCG GGTAGCAGCG GGAAGTCTCC GCGATCTAAA
    CGCAGTGGCC GAATAGCACC ACAGAACCGC AACAGCATGT TAACATCTCT GTGTATTCTG
    AGCCACTACC CTTTCTTCTC AACATTCAGA GAGTGTCTGT ACATCCTCAA GCGCATGGTG
    GACTGCTGTA GTCAGCGGCT CAACCAGAGA CCCGGAGCTC CCAAATCCAC ACAACGGGAC
    ACGATGTGGC GTGTGTTCAC GGGAGCTCTT TCTGTGGAAG AAAAGGAGAA GGGCAGTCAG
    GTGCTCCAGG ACCTGAGGGA GATCGAGTCA TGGGTTTATC GGCTGCTGCG CTCACCGGTG
    CCTGTTGCAG GTCAGCGGCG TGTGGATGTG GAGGTCCTGC CCCATGAACT GCAGCCTGCT
    CTGACATTTG CCCTTCCAGA CCCGTCCCGC TTCAGCATCG TAGACTTCCC GCTCCACCTG
    CCACTGGAGC TGCTGGGAGT CGATGCCTGT CTGCAGGTTT TGGCCTGCAT TCTGCTGGAG
    CACAAGGTGG TACTGCAGTC TCGAGATTAT AACGCTCTCT CAATGAGCGT CATGGCGTTT
    GTTTCGATGA TTTACCCTCT TGAGTACATG TTTCCTGTCA TTCCACTGTT GCCCACCTGC
    ATGGCCTCTG CAGAACAGCT CCTATTGGCA CCCACTCCAT ACGTCATTGG TGTGCCAGCC
    AGTTTCTTCC TGTACAAGTG TGATTTTAAG ATGCCTGATG ATGTTTGGTT AGTGGATCTG
    GACTGCAACA AGGTTATTGT TCCAAGCAAT GCAGAACTGC TCCCACCCTT ACCTGAACCT
    GAGGCCTCTG AGCTGAAGAA ACACTTGAAG CAGCTCTTGG AGTGTCTGGT AAGGTTGACT
    CTGATCACCC AAAAGCAGAT CTTCGCCTCC GATTCTAAGG CTCTGGCTAG CATGAGTCTA
    AACACACAGC CTATTCTGAA CCTGGAGAAG TTTCAGGATG GACAGGAGTT GTCTTTATTG
    CCCCCCGGTC GTGACAAAGC TTCCCCGTCC TCCACTGAGT TCAACCCACT CATCTACGGC
    AATGATGTGG ACTCAGTTGA TGTTGCCACA AGGGTGGCAA TGGTGAGGTT CTTCAACTCT
    CCAAACGTGC TGCAAGGGTT CCAGATGCAC ACACGCACTC TCCGTCTCTT CCCTCGTCCT
    GTGGTGGCCT TTCAGTCCAC ATCTTTCCTG GCTTCTAGGC CACGACGAAA TGGATTCACA
    GAAAAGCTCT CCCACACTCA GGCAGTGGAG TACTATGGAG AATGGGCTCT CAACCCCACC
    AACCTGGCTT TCCAAAGGAT TCATAACAAT GTGTATGACC CCTCTCTAAT AGGCGATAAG
    CCTAAGTGGT ATGCCCACCA GTTACAGCCA GTATTCTACC GCGTGTATGA TGGAAACTCT
    CATTTAGCGG AGGCCTTGAG TGGACCCCTG CAGGATGAGA CCAATGATTC AGACCCAACA
    GACGACAGTG GCAGCGACAG TGAAGCATAT GATGACTCCA GCTCCTCCTA CTCATCTCTT
    GGAGACTTTG TGAATGAGAT GATCAAAGGG GACATACAGG GAGACACACC AAATGTTGAT
    CCCCTGACCC ATGCTGCACT GGGAGATGCA GATGAGGTTG AGATCCATGA CTTTCACGAG
    TATAAGGGGG ACAGCGGTGA ACCAGAGCCA GAAGGCCCCA CAGAAGCGGC GGACAGTCAG
    CCCTTGCGGT CCAGCTCTAG CACCACAGCC AGTTCCAGCC CCAGTACAGT CATACAGGGA
    GTCAACCATG AGCAGAAGGA ACCTGTTGAG GTGGAGCCCA TAGCAAATGT GACTTTCCCA
    AGCTCTGCTC CAGTATTGGG TCCTCCTCCT TTTACAAGAC CCACCCCTGA TCCAACCCTT
    GTTGACCCTG CCAATAAGAA GCGAGAATAT GACAACCCCT ATTTTGAGCC CCAGTACGGT
    TTCCCTACAG AGGAAGATGC TGAGACTGAT GAGCAGGAGG AGAGTTACAC ACCACGATTT
    AACCAGAATC TCAATGGCAA TAAGCCTTCT CGTCCACTGA GACCCAGCAG CCTGAAGCTT
    CCGGGTGAAT CTGACGGAGA GGGAGACTCC AGAAATAGCT CTCCTAACTC AACCATCTCC
    AACAATAGCA GTGATGGCTT TGGGGGACTC ATGTCCTTTG CCAGTAACTT GTATAAAAAC
    CATGGCACCA GTTTCAGTCT GTCCAGCCTG GCTCTGCCTA ATAAAGCGAG AGAGAAGAAC
    ACGCCGTTCC CCAGCCTCAA AGAATATTTT AATTTTGATT TAGCAGAGGA CACAGATCAA
    GACGATGCTG ATGACAGTCC TGAAAGTCCT GTATTTGGGC TAAATTCTCT AATGGAGATT
    GTAACAGAGA TCGGCCCGGG GAGCGGAGAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGAATTGTGG AGCAAGCACC AGGATAACAG AAAGCAAAAA
    GCTTTGGAAA AAGAACAGAG TGGTTTAAAT AAACGGAAAT CAGGAGCGGA TGGAGTAAAA
    CAACGTGTAG AAGGAGGAGA CACTGAGGAG AAGAAATCAC AGATCAGTGC AGACAGTGGC
    CTCAGTGTGA CATCTGGCTC ACAGAAGAGT GATACGGATT CCCTGGCGAG CTCTGAACCT
    CCTGCTCTGA CCAGAAGCAC CAGCCAGGAC TCAGAGGCCA GCACAGTGGT AAGTAACAGC
    TCAGGTGAGA CGCTCGGTGC AGACAGTGAC CTGAGCAGCA CTGCTGGAGA TGGCCTGACA
    GGAAGACATG GTCAGCATCT CAACCTGTCC CGTGGCACAC TGTCTGACAG TGAGATTGAG
    ACCAACCCTG CAACCAGCTC AGTGTTTGGC AAGACACACA AGCTGAAGCC AGGAGTGAAG
    GAGCCTGTTG GTGTTAATAA AGCAGCACCT GCTCCACCCG TTGAGGATGT TAGCATGAGG
    ATCTATCTGT GTGAGGGGCT TCTGGGCCGG GATAAAAGCT CAGTTTGGGA CCAGTTGGAG
    GATGCTGCAA TGGAAACCTT TTCTCTGAGT AAAGAGCGCT CAACTCTGTG GGATCAGATG
    CAGTTCTGGG AGGACGCCTT CCTGGATGCC GTAATGCTGG AACGGGAAGG AATGGGAATG
    GATCAGGGAC CACAAGAAAT GATTGACAGG TATCTTTCTC TGGGAGATCA TGACCGCAAG
    AGACTGGAGG ATGATGAGGA CAGACTGCTG GCCACGCTTC TGCACAATAT GATTGCTTAC
    ATGCTTATGA TGAAGGTCGG TAAAAACGAC ATCAGGAAGA AAGTACGTCG TCTTATGGGC
    AAATCCCACA TTGGCCTCAC TCACAGTCAG GAGATCAATG AAGTCCTGGA TCGCCTCGCA
    CATTTAAGCG GTCGGGAGCT TTCTATCAGG CCGAGTGGCA GCAGGCATAT TAAAAAACAG
    ACATTTGTTG TGCATGCTGG GACAGATACA ACTGGTGACA TATTCTTCAT GGAGGTGTGT
    GATGATTGCA TCGTCTTGCG CAGTAATATT GGCACAGTCT ATGAGCGCTG GTGGTATGAG
    AAGCTCATAA ATATGACTTA CTGTCCCAAA ACTAAAGTGT TGTGTCTGTG GAGGAGAAAC
    GGCCAGGAGA CGCAGCTAAA CAAGTTTTAC ACTAAGAAGT GTCGGGAATT GTACTACTGT
    GTTAAAGACA GCATGGAGCG TGCTGCTGCT CGACAACAAA GCATCAAACC AGGTCCAGAG
    CTTGGAGGCG AGTTTCCGGT TCAGGACATG AAGACAGGGG AGGGTGGTCT CCTCCAAGTC
    ACACTGGAAG GCATCAACCT CAAATTTATG CACAGCCAGG AGCGGAAGGT TTTCATAGAG
    CTGAATCACA TTAAAAAGTG CAATACAGTG AAAGGAGTCT TTGTCCTGGA GGAGTTTGTT
    CCTGAAACTA AAGAAGTGGT GATCCATAAG TACAAGACTC CTATGGCACA TCAGATCTGT
    TACTCGGTGC TCTGCCTCTT CTCCTACATG GCGGCGGTCA AGGGGAAAGA GTCAGAGGGC
    AAACCCAAGA TGCTGTCCCC TCGTCCACTA GCGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144895
    Clone ID Related Accession (Same CDS sequence) XM_021471965.1
    Accession Version XM_021471965.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5073bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394698.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA CTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGGACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCGGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT TCCCAAGCTC TGCTCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTCCCCAGC CTCAAAGAAT ATTTTAATTT TGATTTAGCA
    GAGGACACAG ATCAAGACGA TGCTGATGAC AGTCCTGAAA GTCCTGTATT TGGGCTAAAT
    TCTCTAATGG AGATTGTAAC AGAGATCGGC CCGGGGAGCG GAGAAGGTGC CCGAGCCCCC
    AGAGCACTGG TAGATCAGAA ATCCTCTGTT ATTAAGCACA GCCCTACTGT GAAGAGAGAG
    TCTCCGTCTC CTCAAGGCAG AGCCAACAAT ACCAGTGAGA ACCAGCAGTT TCTAAAGGAG
    GTGGTCCAGA GCGTGCTGGA GGGGCAGGGT GTGGGCTGGC TTAACATGAA GAAGGTGCGT
    CGCCTGCTAG AGAATGAACA GCTCAGAGTC TTTGTGCTCA GCAAACTCAA CCGTGCGGTC
    CAGTCAGAGG AGGACGCCCA GCAGGAAGTT ATAAGAGATG TGGAGATAAA TAGAAAGGTC
    TACAAAGGCA TGCTGGATAT TTTGAAGTGC ACGGTGTCCA GTTTGGAGCA TTCATACACT
    AATGCTGGCT TAGGGGGCAT GGCCAGTGTC TTCAGTCTGC TAGAGATAGC ACGCACCCAC
    TACCAAACCA AAGATCCAGA GAAGCGGAAA CGGAGCCCCA CCGAAGGGCT TAGCAGTCCA
    GGCAGTAAGG AGAGCCCATC GGGCCGAATG GAGAGTGCCA GGGCGGCAGG GGTGCTTCTG
    GTACCCCGTA TCCAATTGCC GCCACCCTCC TCTGGGAAAT CATCACGCCA GTTTGATACC
    CGGAGTCTGA ACGAGGAGAA TTTTATTGCC TCCATTGGAG CGGATGGAGT AAAACAACGT
    GTAGAAGGAG GAGACACTGA GGAGAAGAAA TCACAGATCA GTGCAGACAG TGGCCTCAGT
    GTGACATCTG GCTCACAGAA GAGTGATACG GATTCCCTGG CGAGCTCTGA ACCTCCTGCT
    CTGACCAGAA GCACCAGCCA GGACTCAGAG GCCAGCACAG TGGTAAGTAA CAGCTCAGGT
    GAGACGCTCG GTGCAGACAG TGACCTGAGC AGCACTGCTG GAGATGGCCT GACAGGAAGA
    CATGGTCAGC ATCTCAACCT GTCCCGTGGC ACACTGTCTG ACAGTGAGAT TGAGACCAAC
    CCTGCAACCA GCTCAGTGTT TGGCAAGACA CACAAGCTGA AGCCAGGAGT GAAGGAGCCT
    GTTGGTGTTA ATAAAGCAGC ACCTGCTCCA CCCGTTGAGG ATGTTAGCAT GAGGATCTAT
    CTGTGTGAGG GGCTTCTGGG CCGGGATAAA AGCTCAGTTT GGGACCAGTT GGAGGATGCT
    GCAATGGAAA CCTTTTCTCT GAGTAAAGAG CGCTCAACTC TGTGGGATCA GATGCAGTTC
    TGGGAGGACG CCTTCCTGGA TGCCGTAATG CTGGAACGGG AAGGAATGGG AATGGATCAG
    GGACCACAAG AAATGATTGA CAGGTATCTT TCTCTGGGAG ATCATGACCG CAAGAGACTG
    GAGGATGATG AGGACAGACT GCTGGCCACG CTTCTGCACA ATATGATTGC TTACATGCTT
    ATGATGAAGG TCGGTAAAAA CGACATCAGG AAGAAAGTAC GTCGTCTTAT GGGCAAATCC
    CACATTGGCC TCACTCACAG TCAGGAGATC AATGAAGTCC TGGATCGCCT CGCACATTTA
    AGCGGTCGGG AGCTTTCTAT CAGGCCGAGT GGCAGCAGGC ATATTAAAAA ACAGACATTT
    GTTGTGCATG CTGGGACAGA TACAACTGGT GACATATTCT TCATGGAGGT GTGTGATGAT
    TGCATCGTCT TGCGCAGTAA TATTGGCACA GTCTATGAGC GCTGGTGGTA TGAGAAGCTC
    ATAAATATGA CTTACTGTCC CAAAACTAAA GTGTTGTGTC TGTGGAGGAG AAACGGCCAG
    GAGACGCAGC TAAACAAGTT TTACACTAAG AAGTGTCGGG AATTGTACTA CTGTGTTAAA
    GACAGCATGG AGCGTGCTGC TGCTCGACAA CAAAGCATCA AACCAGGTCC AGAGCTTGGA
    GGCGAGTTTC CGGTTCAGGA CATGAAGACA GGGGAGGGTG GTCTCCTCCA AGTCACACTG
    GAAGGCATCA ACCTCAAATT TATGCACAGC CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT
    CACATTAAAA AGTGCAATAC AGTGAAAGGA GTCTTTGTCC TGGAGGAGTT TGTTCCTGAA
    ACTAAAGAAG TGGTGATCCA TAAGTACAAG ACTCCTATGG CACATCAGAT CTGTTACTCG
    GTGCTCTGCC TCTTCTCCTA CATGGCGGCG GTCAAGGGGA AAGAGTCAGA GGGCAAACCC
    AAGATGCTGT CCCCTCGTCC ACTAGCGAGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327640.1
    CDS169..5241
    Translation

    Target ORF information:

    RefSeq Version XM_021471965.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X54, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471965.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA CTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGGACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCGGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT TCCCAAGCTC TGCTCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTCCCCAGC CTCAAAGAAT ATTTTAATTT TGATTTAGCA
    GAGGACACAG ATCAAGACGA TGCTGATGAC AGTCCTGAAA GTCCTGTATT TGGGCTAAAT
    TCTCTAATGG AGATTGTAAC AGAGATCGGC CCGGGGAGCG GAGAAGGTGC CCGAGCCCCC
    AGAGCACTGG TAGATCAGAA ATCCTCTGTT ATTAAGCACA GCCCTACTGT GAAGAGAGAG
    TCTCCGTCTC CTCAAGGCAG AGCCAACAAT ACCAGTGAGA ACCAGCAGTT TCTAAAGGAG
    GTGGTCCAGA GCGTGCTGGA GGGGCAGGGT GTGGGCTGGC TTAACATGAA GAAGGTGCGT
    CGCCTGCTAG AGAATGAACA GCTCAGAGTC TTTGTGCTCA GCAAACTCAA CCGTGCGGTC
    CAGTCAGAGG AGGACGCCCA GCAGGAAGTT ATAAGAGATG TGGAGATAAA TAGAAAGGTC
    TACAAAGGCA TGCTGGATAT TTTGAAGTGC ACGGTGTCCA GTTTGGAGCA TTCATACACT
    AATGCTGGCT TAGGGGGCAT GGCCAGTGTC TTCAGTCTGC TAGAGATAGC ACGCACCCAC
    TACCAAACCA AAGATCCAGA GAAGCGGAAA CGGAGCCCCA CCGAAGGGCT TAGCAGTCCA
    GGCAGTAAGG AGAGCCCATC GGGCCGAATG GAGAGTGCCA GGGCGGCAGG GGTGCTTCTG
    GTACCCCGTA TCCAATTGCC GCCACCCTCC TCTGGGAAAT CATCACGCCA GTTTGATACC
    CGGAGTCTGA ACGAGGAGAA TTTTATTGCC TCCATTGGAG CGGATGGAGT AAAACAACGT
    GTAGAAGGAG GAGACACTGA GGAGAAGAAA TCACAGATCA GTGCAGACAG TGGCCTCAGT
    GTGACATCTG GCTCACAGAA GAGTGATACG GATTCCCTGG CGAGCTCTGA ACCTCCTGCT
    CTGACCAGAA GCACCAGCCA GGACTCAGAG GCCAGCACAG TGGTAAGTAA CAGCTCAGGT
    GAGACGCTCG GTGCAGACAG TGACCTGAGC AGCACTGCTG GAGATGGCCT GACAGGAAGA
    CATGGTCAGC ATCTCAACCT GTCCCGTGGC ACACTGTCTG ACAGTGAGAT TGAGACCAAC
    CCTGCAACCA GCTCAGTGTT TGGCAAGACA CACAAGCTGA AGCCAGGAGT GAAGGAGCCT
    GTTGGTGTTA ATAAAGCAGC ACCTGCTCCA CCCGTTGAGG ATGTTAGCAT GAGGATCTAT
    CTGTGTGAGG GGCTTCTGGG CCGGGATAAA AGCTCAGTTT GGGACCAGTT GGAGGATGCT
    GCAATGGAAA CCTTTTCTCT GAGTAAAGAG CGCTCAACTC TGTGGGATCA GATGCAGTTC
    TGGGAGGACG CCTTCCTGGA TGCCGTAATG CTGGAACGGG AAGGAATGGG AATGGATCAG
    GGACCACAAG AAATGATTGA CAGGTATCTT TCTCTGGGAG ATCATGACCG CAAGAGACTG
    GAGGATGATG AGGACAGACT GCTGGCCACG CTTCTGCACA ATATGATTGC TTACATGCTT
    ATGATGAAGG TCGGTAAAAA CGACATCAGG AAGAAAGTAC GTCGTCTTAT GGGCAAATCC
    CACATTGGCC TCACTCACAG TCAGGAGATC AATGAAGTCC TGGATCGCCT CGCACATTTA
    AGCGGTCGGG AGCTTTCTAT CAGGCCGAGT GGCAGCAGGC ATATTAAAAA ACAGACATTT
    GTTGTGCATG CTGGGACAGA TACAACTGGT GACATATTCT TCATGGAGGT GTGTGATGAT
    TGCATCGTCT TGCGCAGTAA TATTGGCACA GTCTATGAGC GCTGGTGGTA TGAGAAGCTC
    ATAAATATGA CTTACTGTCC CAAAACTAAA GTGTTGTGTC TGTGGAGGAG AAACGGCCAG
    GAGACGCAGC TAAACAAGTT TTACACTAAG AAGTGTCGGG AATTGTACTA CTGTGTTAAA
    GACAGCATGG AGCGTGCTGC TGCTCGACAA CAAAGCATCA AACCAGGTCC AGAGCTTGGA
    GGCGAGTTTC CGGTTCAGGA CATGAAGACA GGGGAGGGTG GTCTCCTCCA AGTCACACTG
    GAAGGCATCA ACCTCAAATT TATGCACAGC CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT
    CACATTAAAA AGTGCAATAC AGTGAAAGGA GTCTTTGTCC TGGAGGAGTT TGTTCCTGAA
    ACTAAAGAAG TGGTGATCCA TAAGTACAAG ACTCCTATGG CACATCAGAT CTGTTACTCG
    GTGCTCTGCC TCTTCTCCTA CATGGCGGCG GTCAAGGGGA AAGAGTCAGA GGGCAAACCC
    AAGATGCTGT CCCCTCGTCC ACTAGCGAGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144896
    Clone ID Related Accession (Same CDS sequence) XM_021471966.1
    Accession Version XM_021471966.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5055bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394698.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA CTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGGACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCGGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT TCCCAAGCTC TGCTCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTCCCCAGC CTCAAAGACG ATGCTGATGA CAGTCCTGAA
    AGTCCTGGTG CCCGAGCCCC CAGAGCACTG GTAGATCAGA AATCCTCTGT TATTAAGCAC
    AGCCCTACTG TGAAGAGAGA GTCTCCGTCT CCTCAAGGCA GAGCCAACAA TACCAGTGAG
    AACCAGCAGT TTCTAAAGGA GGTGGTCCAG AGCGTGCTGG AGGGGCAGGG TGTGGGCTGG
    CTTAACATGA AGAAGGTGCG TCGCCTGCTA GAGAATGAAC AGCTCAGAGT CTTTGTGCTC
    AGCAAACTCA ACCGTGCGGT CCAGTCAGAG GAGGACGCCC AGCAGGAAGT TATAAGAGAT
    GTGGAGATAA ATAGAAAGGT CTACAAAGGC ATGCTGGATA TTTTGAAGTG CACGGTGTCC
    AGTTTGGAGC ATTCATACAC TAATGCTGGC TTAGGGGGCA TGGCCAGTGT CTTCAGTCTG
    CTAGAGATAG CACGCACCCA CTACCAAACC AAAGATCCAG AGAAGCGGAA ACGGAGCCCC
    ACCGAAGGGC TTAGCAGTCC AGGCAGTAAG GAGAGCCCAT CGGGCCGAAT GGAGAGTGCC
    AGGGCGGCAG GGGTGCTTCT GGTACCCCGT ATCCAATTGC CGCCACCCTC CTCTGGGAAA
    TCATCACGCC AGTTTGATAC CCGGAGTCTG AACGAGGAGA ATTTTATTGC CTCCATTGAA
    TTGTGGAGCA AGCACCAGGA TAACAGAAAG CAAAAAGCTT TGGAAAAAGA ACAGAGTGGT
    TTAAATAAAC GGAAATCAGG AGCGGATGGA GTAAAACAAC GTGTAGAAGG AGGAGACACT
    GAGGAGAAGA AATCACAGAT CAGTGCAGAC AGTGGCCTCA GTGTGACATC TGGCTCACAG
    AAGAGTGATA CGGATTCCCT GGCGAGCTCT GAACCTCCTG CTCTGACCAG AAGCACCAGC
    CAGGACTCAG AGGCCAGCAC AGTGGTAAGT AACAGCTCAG GTGAGACGCT CGGTGCAGAC
    AGTGACCTGA GCAGCACTGC TGGAGATGGC CTGACAGGAA GACATGGTCA GCATCTCAAC
    CTGTCCCGTG GCACACTGTC TGACAGTGAG ATTGAGACCA ACCCTGCAAC CAGCTCAGTG
    TTTGGCAAGA CACACAAGCT GAAGCCAGGA GTGAAGGAGC CTGTTGGTGT TAATAAAGCA
    GCACCTGCTC CACCCGTTGA GGATGTTAGC ATGAGGATCT ATCTGTGTGA GGGGCTTCTG
    GGCCGGGATA AAAGCTCAGT TTGGGACCAG TTGGAGGATG CTGCAATGGA AACCTTTTCT
    CTGAGTAAAG AGCGCTCAAC TCTGTGGGAT CAGATGCAGT TCTGGGAGGA CGCCTTCCTG
    GATGCCGTAA TGCTGGAACG GGAAGGAATG GGAATGGATC AGGGACCACA AGAAATGATT
    GACAGGTATC TTTCTCTGGG AGATCATGAC CGCAAGAGAC TGGAGGATGA TGAGGACAGA
    CTGCTGGCCA CGCTTCTGCA CAATATGATT GCTTACATGC TTATGATGAA GGTCGGTAAA
    AACGACATCA GGAAGAAAGT ACGTCGTCTT ATGGGCAAAT CCCACATTGG CCTCACTCAC
    AGTCAGGAGA TCAATGAAGT CCTGGATCGC CTCGCACATT TAAGCGGTCG GGAGCTTTCT
    ATCAGGCCGA GTGGCAGCAG GCATATTAAA AAACAGACAT TTGTTGTGCA TGCTGGGACA
    GATACAACTG GTGACATATT CTTCATGGAG GTGTGTGATG ATTGCATCGT CTTGCGCAGT
    AATATTGGCA CAGTCTATGA GCGCTGGTGG TATGAGAAGC TCATAAATAT GACTTACTGT
    CCCAAAACTA AAGTGTTGTG TCTGTGGAGG AGAAACGGCC AGGAGACGCA GCTAAACAAG
    TTTTACACTA AGAAGTGTCG GGAATTGTAC TACTGTGTTA AAGACAGCAT GGAGCGTGCT
    GCTGCTCGAC AACAAAGCAT CAAACCAGGT CCAGAGCTTG GAGGCGAGTT TCCGGTTCAG
    GACATGAAGA CAGGGGAGGG TGGTCTCCTC CAAGTCACAC TGGAAGGCAT CAACCTCAAA
    TTTATGCACA GCCAGGAGCG GAAGGTTTTC ATAGAGCTGA ATCACATTAA AAAGTGCAAT
    ACAGTGAAAG GAGTCTTTGT CCTGGAGGAG TTTGTTCCTG AAACTAAAGA AGTGGTGATC
    CATAAGTACA AGACTCCTAT GGCACATCAG ATCTGTTACT CGGTGCTCTG CCTCTTCTCC
    TACATGGCGG CGGTCAAGGG GAAAGAGTCA GAGGGCAAAC CCAAGATGCT GTCCCCTCGT
    CCACTAGCGA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327641.1
    CDS169..5223
    Translation

    Target ORF information:

    RefSeq Version XM_021471966.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X55, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471966.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA CTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGGACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCGGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT TCCCAAGCTC TGCTCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTCCCCAGC CTCAAAGACG ATGCTGATGA CAGTCCTGAA
    AGTCCTGGTG CCCGAGCCCC CAGAGCACTG GTAGATCAGA AATCCTCTGT TATTAAGCAC
    AGCCCTACTG TGAAGAGAGA GTCTCCGTCT CCTCAAGGCA GAGCCAACAA TACCAGTGAG
    AACCAGCAGT TTCTAAAGGA GGTGGTCCAG AGCGTGCTGG AGGGGCAGGG TGTGGGCTGG
    CTTAACATGA AGAAGGTGCG TCGCCTGCTA GAGAATGAAC AGCTCAGAGT CTTTGTGCTC
    AGCAAACTCA ACCGTGCGGT CCAGTCAGAG GAGGACGCCC AGCAGGAAGT TATAAGAGAT
    GTGGAGATAA ATAGAAAGGT CTACAAAGGC ATGCTGGATA TTTTGAAGTG CACGGTGTCC
    AGTTTGGAGC ATTCATACAC TAATGCTGGC TTAGGGGGCA TGGCCAGTGT CTTCAGTCTG
    CTAGAGATAG CACGCACCCA CTACCAAACC AAAGATCCAG AGAAGCGGAA ACGGAGCCCC
    ACCGAAGGGC TTAGCAGTCC AGGCAGTAAG GAGAGCCCAT CGGGCCGAAT GGAGAGTGCC
    AGGGCGGCAG GGGTGCTTCT GGTACCCCGT ATCCAATTGC CGCCACCCTC CTCTGGGAAA
    TCATCACGCC AGTTTGATAC CCGGAGTCTG AACGAGGAGA ATTTTATTGC CTCCATTGAA
    TTGTGGAGCA AGCACCAGGA TAACAGAAAG CAAAAAGCTT TGGAAAAAGA ACAGAGTGGT
    TTAAATAAAC GGAAATCAGG AGCGGATGGA GTAAAACAAC GTGTAGAAGG AGGAGACACT
    GAGGAGAAGA AATCACAGAT CAGTGCAGAC AGTGGCCTCA GTGTGACATC TGGCTCACAG
    AAGAGTGATA CGGATTCCCT GGCGAGCTCT GAACCTCCTG CTCTGACCAG AAGCACCAGC
    CAGGACTCAG AGGCCAGCAC AGTGGTAAGT AACAGCTCAG GTGAGACGCT CGGTGCAGAC
    AGTGACCTGA GCAGCACTGC TGGAGATGGC CTGACAGGAA GACATGGTCA GCATCTCAAC
    CTGTCCCGTG GCACACTGTC TGACAGTGAG ATTGAGACCA ACCCTGCAAC CAGCTCAGTG
    TTTGGCAAGA CACACAAGCT GAAGCCAGGA GTGAAGGAGC CTGTTGGTGT TAATAAAGCA
    GCACCTGCTC CACCCGTTGA GGATGTTAGC ATGAGGATCT ATCTGTGTGA GGGGCTTCTG
    GGCCGGGATA AAAGCTCAGT TTGGGACCAG TTGGAGGATG CTGCAATGGA AACCTTTTCT
    CTGAGTAAAG AGCGCTCAAC TCTGTGGGAT CAGATGCAGT TCTGGGAGGA CGCCTTCCTG
    GATGCCGTAA TGCTGGAACG GGAAGGAATG GGAATGGATC AGGGACCACA AGAAATGATT
    GACAGGTATC TTTCTCTGGG AGATCATGAC CGCAAGAGAC TGGAGGATGA TGAGGACAGA
    CTGCTGGCCA CGCTTCTGCA CAATATGATT GCTTACATGC TTATGATGAA GGTCGGTAAA
    AACGACATCA GGAAGAAAGT ACGTCGTCTT ATGGGCAAAT CCCACATTGG CCTCACTCAC
    AGTCAGGAGA TCAATGAAGT CCTGGATCGC CTCGCACATT TAAGCGGTCG GGAGCTTTCT
    ATCAGGCCGA GTGGCAGCAG GCATATTAAA AAACAGACAT TTGTTGTGCA TGCTGGGACA
    GATACAACTG GTGACATATT CTTCATGGAG GTGTGTGATG ATTGCATCGT CTTGCGCAGT
    AATATTGGCA CAGTCTATGA GCGCTGGTGG TATGAGAAGC TCATAAATAT GACTTACTGT
    CCCAAAACTA AAGTGTTGTG TCTGTGGAGG AGAAACGGCC AGGAGACGCA GCTAAACAAG
    TTTTACACTA AGAAGTGTCG GGAATTGTAC TACTGTGTTA AAGACAGCAT GGAGCGTGCT
    GCTGCTCGAC AACAAAGCAT CAAACCAGGT CCAGAGCTTG GAGGCGAGTT TCCGGTTCAG
    GACATGAAGA CAGGGGAGGG TGGTCTCCTC CAAGTCACAC TGGAAGGCAT CAACCTCAAA
    TTTATGCACA GCCAGGAGCG GAAGGTTTTC ATAGAGCTGA ATCACATTAA AAAGTGCAAT
    ACAGTGAAAG GAGTCTTTGT CCTGGAGGAG TTTGTTCCTG AAACTAAAGA AGTGGTGATC
    CATAAGTACA AGACTCCTAT GGCACATCAG ATCTGTTACT CGGTGCTCTG CCTCTTCTCC
    TACATGGCGG CGGTCAAGGG GAAAGAGTCA GAGGGCAAAC CCAAGATGCT GTCCCCTCGT
    CCACTAGCGA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144897
    Clone ID Related Accession (Same CDS sequence) XM_021471968.1
    Accession Version XM_021471968.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5025bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394698.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA CTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGGACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCGGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT TCCCAAGCTC TGCTCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTCCCCAGC CTCAAAGGTG CCCGAGCCCC CAGAGCACTG
    GTAGATCAGA AATCCTCTGT TATTAAGCAC AGCCCTACTG TGAAGAGAGA GTCTCCGTCT
    CCTCAAGGCA GAGCCAACAA TACCAGTGAG AACCAGCAGT TTCTAAAGGA GGTGGTCCAG
    AGCGTGCTGG AGGGGCAGGG TGTGGGCTGG CTTAACATGA AGAAGGTGCG TCGCCTGCTA
    GAGAATGAAC AGCTCAGAGT CTTTGTGCTC AGCAAACTCA ACCGTGCGGT CCAGTCAGAG
    GAGGACGCCC AGCAGGAAGT TATAAGAGAT GTGGAGATAA ATAGAAAGGT CTACAAAGGC
    ATGCTGGATA TTTTGAAGTG CACGGTGTCC AGTTTGGAGC ATTCATACAC TAATGCTGGC
    TTAGGGGGCA TGGCCAGTGT CTTCAGTCTG CTAGAGATAG CACGCACCCA CTACCAAACC
    AAAGATCCAG AGAAGCGGAA ACGGAGCCCC ACCGAAGGGC TTAGCAGTCC AGGCAGTAAG
    GAGAGCCCAT CGGGCCGAAT GGAGAGTGCC AGGGCGGCAG GGGTGCTTCT GGTACCCCGT
    ATCCAATTGC CGCCACCCTC CTCTGGGAAA TCATCACGCC AGTTTGATAC CCGGAGTCTG
    AACGAGGAGA ATTTTATTGC CTCCATTGAA TTGTGGAGCA AGCACCAGGA TAACAGAAAG
    CAAAAAGCTT TGGAAAAAGA ACAGAGTGGT TTAAATAAAC GGAAATCAGG AGCGGATGGA
    GTAAAACAAC GTGTAGAAGG AGGAGACACT GAGGAGAAGA AATCACAGAT CAGTGCAGAC
    AGTGGCCTCA GTGTGACATC TGGCTCACAG AAGAGTGATA CGGATTCCCT GGCGAGCTCT
    GAACCTCCTG CTCTGACCAG AAGCACCAGC CAGGACTCAG AGGCCAGCAC AGTGGTAAGT
    AACAGCTCAG GTGAGACGCT CGGTGCAGAC AGTGACCTGA GCAGCACTGC TGGAGATGGC
    CTGACAGGAA GACATGGTCA GCATCTCAAC CTGTCCCGTG GCACACTGTC TGACAGTGAG
    ATTGAGACCA ACCCTGCAAC CAGCTCAGTG TTTGGCAAGA CACACAAGCT GAAGCCAGGA
    GTGAAGGAGC CTGTTGGTGT TAATAAAGCA GCACCTGCTC CACCCGTTGA GGATGTTAGC
    ATGAGGATCT ATCTGTGTGA GGGGCTTCTG GGCCGGGATA AAAGCTCAGT TTGGGACCAG
    TTGGAGGATG CTGCAATGGA AACCTTTTCT CTGAGTAAAG AGCGCTCAAC TCTGTGGGAT
    CAGATGCAGT TCTGGGAGGA CGCCTTCCTG GATGCCGTAA TGCTGGAACG GGAAGGAATG
    GGAATGGATC AGGGACCACA AGAAATGATT GACAGGTATC TTTCTCTGGG AGATCATGAC
    CGCAAGAGAC TGGAGGATGA TGAGGACAGA CTGCTGGCCA CGCTTCTGCA CAATATGATT
    GCTTACATGC TTATGATGAA GGTCGGTAAA AACGACATCA GGAAGAAAGT ACGTCGTCTT
    ATGGGCAAAT CCCACATTGG CCTCACTCAC AGTCAGGAGA TCAATGAAGT CCTGGATCGC
    CTCGCACATT TAAGCGGTCG GGAGCTTTCT ATCAGGCCGA GTGGCAGCAG GCATATTAAA
    AAACAGACAT TTGTTGTGCA TGCTGGGACA GATACAACTG GTGACATATT CTTCATGGAG
    GTGTGTGATG ATTGCATCGT CTTGCGCAGT AATATTGGCA CAGTCTATGA GCGCTGGTGG
    TATGAGAAGC TCATAAATAT GACTTACTGT CCCAAAACTA AAGTGTTGTG TCTGTGGAGG
    AGAAACGGCC AGGAGACGCA GCTAAACAAG TTTTACACTA AGAAGTGTCG GGAATTGTAC
    TACTGTGTTA AAGACAGCAT GGAGCGTGCT GCTGCTCGAC AACAAAGCAT CAAACCAGGT
    CCAGAGCTTG GAGGCGAGTT TCCGGTTCAG GACATGAAGA CAGGGGAGGG TGGTCTCCTC
    CAAGTCACAC TGGAAGGCAT CAACCTCAAA TTTATGCACA GCCAGGAGCG GAAGGTTTTC
    ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTGAAAG GAGTCTTTGT CCTGGAGGAG
    TTTGTTCCTG AAACTAAAGA AGTGGTGATC CATAAGTACA AGACTCCTAT GGCACATCAG
    ATCTGTTACT CGGTGCTCTG CCTCTTCTCC TACATGGCGG CGGTCAAGGG GAAAGAGTCA
    GAGGGCAAAC CCAAGATGCT GTCCCCTCGT CCACTAGCGA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327643.1
    CDS169..5193
    Translation

    Target ORF information:

    RefSeq Version XM_021471968.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X56, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471968.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA CTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGGACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCGGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT TCCCAAGCTC TGCTCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTCCCCAGC CTCAAAGGTG CCCGAGCCCC CAGAGCACTG
    GTAGATCAGA AATCCTCTGT TATTAAGCAC AGCCCTACTG TGAAGAGAGA GTCTCCGTCT
    CCTCAAGGCA GAGCCAACAA TACCAGTGAG AACCAGCAGT TTCTAAAGGA GGTGGTCCAG
    AGCGTGCTGG AGGGGCAGGG TGTGGGCTGG CTTAACATGA AGAAGGTGCG TCGCCTGCTA
    GAGAATGAAC AGCTCAGAGT CTTTGTGCTC AGCAAACTCA ACCGTGCGGT CCAGTCAGAG
    GAGGACGCCC AGCAGGAAGT TATAAGAGAT GTGGAGATAA ATAGAAAGGT CTACAAAGGC
    ATGCTGGATA TTTTGAAGTG CACGGTGTCC AGTTTGGAGC ATTCATACAC TAATGCTGGC
    TTAGGGGGCA TGGCCAGTGT CTTCAGTCTG CTAGAGATAG CACGCACCCA CTACCAAACC
    AAAGATCCAG AGAAGCGGAA ACGGAGCCCC ACCGAAGGGC TTAGCAGTCC AGGCAGTAAG
    GAGAGCCCAT CGGGCCGAAT GGAGAGTGCC AGGGCGGCAG GGGTGCTTCT GGTACCCCGT
    ATCCAATTGC CGCCACCCTC CTCTGGGAAA TCATCACGCC AGTTTGATAC CCGGAGTCTG
    AACGAGGAGA ATTTTATTGC CTCCATTGAA TTGTGGAGCA AGCACCAGGA TAACAGAAAG
    CAAAAAGCTT TGGAAAAAGA ACAGAGTGGT TTAAATAAAC GGAAATCAGG AGCGGATGGA
    GTAAAACAAC GTGTAGAAGG AGGAGACACT GAGGAGAAGA AATCACAGAT CAGTGCAGAC
    AGTGGCCTCA GTGTGACATC TGGCTCACAG AAGAGTGATA CGGATTCCCT GGCGAGCTCT
    GAACCTCCTG CTCTGACCAG AAGCACCAGC CAGGACTCAG AGGCCAGCAC AGTGGTAAGT
    AACAGCTCAG GTGAGACGCT CGGTGCAGAC AGTGACCTGA GCAGCACTGC TGGAGATGGC
    CTGACAGGAA GACATGGTCA GCATCTCAAC CTGTCCCGTG GCACACTGTC TGACAGTGAG
    ATTGAGACCA ACCCTGCAAC CAGCTCAGTG TTTGGCAAGA CACACAAGCT GAAGCCAGGA
    GTGAAGGAGC CTGTTGGTGT TAATAAAGCA GCACCTGCTC CACCCGTTGA GGATGTTAGC
    ATGAGGATCT ATCTGTGTGA GGGGCTTCTG GGCCGGGATA AAAGCTCAGT TTGGGACCAG
    TTGGAGGATG CTGCAATGGA AACCTTTTCT CTGAGTAAAG AGCGCTCAAC TCTGTGGGAT
    CAGATGCAGT TCTGGGAGGA CGCCTTCCTG GATGCCGTAA TGCTGGAACG GGAAGGAATG
    GGAATGGATC AGGGACCACA AGAAATGATT GACAGGTATC TTTCTCTGGG AGATCATGAC
    CGCAAGAGAC TGGAGGATGA TGAGGACAGA CTGCTGGCCA CGCTTCTGCA CAATATGATT
    GCTTACATGC TTATGATGAA GGTCGGTAAA AACGACATCA GGAAGAAAGT ACGTCGTCTT
    ATGGGCAAAT CCCACATTGG CCTCACTCAC AGTCAGGAGA TCAATGAAGT CCTGGATCGC
    CTCGCACATT TAAGCGGTCG GGAGCTTTCT ATCAGGCCGA GTGGCAGCAG GCATATTAAA
    AAACAGACAT TTGTTGTGCA TGCTGGGACA GATACAACTG GTGACATATT CTTCATGGAG
    GTGTGTGATG ATTGCATCGT CTTGCGCAGT AATATTGGCA CAGTCTATGA GCGCTGGTGG
    TATGAGAAGC TCATAAATAT GACTTACTGT CCCAAAACTA AAGTGTTGTG TCTGTGGAGG
    AGAAACGGCC AGGAGACGCA GCTAAACAAG TTTTACACTA AGAAGTGTCG GGAATTGTAC
    TACTGTGTTA AAGACAGCAT GGAGCGTGCT GCTGCTCGAC AACAAAGCAT CAAACCAGGT
    CCAGAGCTTG GAGGCGAGTT TCCGGTTCAG GACATGAAGA CAGGGGAGGG TGGTCTCCTC
    CAAGTCACAC TGGAAGGCAT CAACCTCAAA TTTATGCACA GCCAGGAGCG GAAGGTTTTC
    ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTGAAAG GAGTCTTTGT CCTGGAGGAG
    TTTGTTCCTG AAACTAAAGA AGTGGTGATC CATAAGTACA AGACTCCTAT GGCACATCAG
    ATCTGTTACT CGGTGCTCTG CCTCTTCTCC TACATGGCGG CGGTCAAGGG GAAAGAGTCA
    GAGGGCAAAC CCAAGATGCT GTCCCCTCGT CCACTAGCGA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144898
    Clone ID Related Accession (Same CDS sequence) XM_021471969.1
    Accession Version XM_021471969.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5019bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394698.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGACTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGGA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCGGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTTCCCAA GCTCTGCTCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTCCC CAGCCTCAAA GTATTTGGGC TAAATTCTCT AATGGAGATT
    GTAACAGAGA TCGGCCCGGG GAGCGGAGAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGAATTGTGG AGCAAGCACC AGGATAACAG AAAGCAAAAA
    GCTTTGGAAA AAGAACAGAG TGGTTTAAAT AAACGGAAAT CAGGAGCGGA TGGAGTAAAA
    CAACGTGTAG AAGGAGGAGA CACTGAGGAG AAGAAATCAC AGATCAGTGC AGACAGTGGC
    CTCAGTGTGA CATCTGGCTC ACAGAAGAGT GATACGGATT CCCTGGCGAG CTCTGAACCT
    CCTGCTCTGA CCAGAAGCAC CAGCCAGGAC TCAGAGGCCA GCACAGTGGT AAGTAACAGC
    TCAGGTGAGA CGCTCGGTGC AGACAGTGAC CTGAGCAGCA CTGCTGGAGA TGGCCTGACA
    GGAAGACATG GTCAGCATCT CAACCTGTCC CGTGGCACAC TGTCTGACAG TGAGATTGAG
    ACCAACCCTG CAACCAGCTC AGTGTTTGGC AAGACACACA AGCTGAAGCC AGGAGTGAAG
    GAGCCTGTTG GTGTTAATAA AGCAGCACCT GCTCCACCCG TTGAGGATGT TAGCATGAGG
    ATCTATCTGT GTGAGGGGCT TCTGGGCCGG GATAAAAGCT CAGTTTGGGA CCAGTTGGAG
    GATGCTGCAA TGGAAACCTT TTCTCTGAGT AAAGAGCGCT CAACTCTGTG GGATCAGATG
    CAGTTCTGGG AGGACGCCTT CCTGGATGCC GTAATGCTGG AACGGGAAGG AATGGGAATG
    GATCAGGGAC CACAAGAAAT GATTGACAGG TATCTTTCTC TGGGAGATCA TGACCGCAAG
    AGACTGGAGG ATGATGAGGA CAGACTGCTG GCCACGCTTC TGCACAATAT GATTGCTTAC
    ATGCTTATGA TGAAGGTCGG TAAAAACGAC ATCAGGAAGA AAGTACGTCG TCTTATGGGC
    AAATCCCACA TTGGCCTCAC TCACAGTCAG GAGATCAATG AAGTCCTGGA TCGCCTCGCA
    CATTTAAGCG GTCGGGAGCT TTCTATCAGG CCGAGTGGCA GCAGGCATAT TAAAAAACAG
    ACATTTGTTG TGCATGCTGG GACAGATACA ACTGGTGACA TATTCTTCAT GGAGGTGTGT
    GATGATTGCA TCGTCTTGCG CAGTAATATT GGCACAGTCT ATGAGCGCTG GTGGTATGAG
    AAGCTCATAA ATATGACTTA CTGTCCCAAA ACTAAAGTGT TGTGTCTGTG GAGGAGAAAC
    GGCCAGGAGA CGCAGCTAAA CAAGTTTTAC ACTAAGAAGT GTCGGGAATT GTACTACTGT
    GTTAAAGACA GCATGGAGCG TGCTGCTGCT CGACAACAAA GCATCAAACC AGGTCCAGAG
    CTTGGAGGCG AGTTTCCGGT TCAGGACATG AAGACAGGGG AGGGTGGTCT CCTCCAAGTC
    ACACTGGAAG GCATCAACCT CAAATTTATG CACAGCCAGG AGCGGAAGGT TTTCATAGAG
    CTGAATCACA TTAAAAAGTG CAATACAGTG AAAGGAGTCT TTGTCCTGGA GGAGTTTGTT
    CCTGAAACTA AAGAAGTGGT GATCCATAAG TACAAGACTC CTATGGCACA TCAGATCTGT
    TACTCGGTGC TCTGCCTCTT CTCCTACATG GCGGCGGTCA AGGGGAAAGA GTCAGAGGGC
    AAACCCAAGA TGCTGTCCCC TCGTCCACTA GCGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327644.1
    CDS1..5019
    Translation

    Target ORF information:

    RefSeq Version XM_021471969.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X57, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471969.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGACTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGGA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCGGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTTCCCAA GCTCTGCTCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTCCC CAGCCTCAAA GTATTTGGGC TAAATTCTCT AATGGAGATT
    GTAACAGAGA TCGGCCCGGG GAGCGGAGAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGAATTGTGG AGCAAGCACC AGGATAACAG AAAGCAAAAA
    GCTTTGGAAA AAGAACAGAG TGGTTTAAAT AAACGGAAAT CAGGAGCGGA TGGAGTAAAA
    CAACGTGTAG AAGGAGGAGA CACTGAGGAG AAGAAATCAC AGATCAGTGC AGACAGTGGC
    CTCAGTGTGA CATCTGGCTC ACAGAAGAGT GATACGGATT CCCTGGCGAG CTCTGAACCT
    CCTGCTCTGA CCAGAAGCAC CAGCCAGGAC TCAGAGGCCA GCACAGTGGT AAGTAACAGC
    TCAGGTGAGA CGCTCGGTGC AGACAGTGAC CTGAGCAGCA CTGCTGGAGA TGGCCTGACA
    GGAAGACATG GTCAGCATCT CAACCTGTCC CGTGGCACAC TGTCTGACAG TGAGATTGAG
    ACCAACCCTG CAACCAGCTC AGTGTTTGGC AAGACACACA AGCTGAAGCC AGGAGTGAAG
    GAGCCTGTTG GTGTTAATAA AGCAGCACCT GCTCCACCCG TTGAGGATGT TAGCATGAGG
    ATCTATCTGT GTGAGGGGCT TCTGGGCCGG GATAAAAGCT CAGTTTGGGA CCAGTTGGAG
    GATGCTGCAA TGGAAACCTT TTCTCTGAGT AAAGAGCGCT CAACTCTGTG GGATCAGATG
    CAGTTCTGGG AGGACGCCTT CCTGGATGCC GTAATGCTGG AACGGGAAGG AATGGGAATG
    GATCAGGGAC CACAAGAAAT GATTGACAGG TATCTTTCTC TGGGAGATCA TGACCGCAAG
    AGACTGGAGG ATGATGAGGA CAGACTGCTG GCCACGCTTC TGCACAATAT GATTGCTTAC
    ATGCTTATGA TGAAGGTCGG TAAAAACGAC ATCAGGAAGA AAGTACGTCG TCTTATGGGC
    AAATCCCACA TTGGCCTCAC TCACAGTCAG GAGATCAATG AAGTCCTGGA TCGCCTCGCA
    CATTTAAGCG GTCGGGAGCT TTCTATCAGG CCGAGTGGCA GCAGGCATAT TAAAAAACAG
    ACATTTGTTG TGCATGCTGG GACAGATACA ACTGGTGACA TATTCTTCAT GGAGGTGTGT
    GATGATTGCA TCGTCTTGCG CAGTAATATT GGCACAGTCT ATGAGCGCTG GTGGTATGAG
    AAGCTCATAA ATATGACTTA CTGTCCCAAA ACTAAAGTGT TGTGTCTGTG GAGGAGAAAC
    GGCCAGGAGA CGCAGCTAAA CAAGTTTTAC ACTAAGAAGT GTCGGGAATT GTACTACTGT
    GTTAAAGACA GCATGGAGCG TGCTGCTGCT CGACAACAAA GCATCAAACC AGGTCCAGAG
    CTTGGAGGCG AGTTTCCGGT TCAGGACATG AAGACAGGGG AGGGTGGTCT CCTCCAAGTC
    ACACTGGAAG GCATCAACCT CAAATTTATG CACAGCCAGG AGCGGAAGGT TTTCATAGAG
    CTGAATCACA TTAAAAAGTG CAATACAGTG AAAGGAGTCT TTGTCCTGGA GGAGTTTGTT
    CCTGAAACTA AAGAAGTGGT GATCCATAAG TACAAGACTC CTATGGCACA TCAGATCTGT
    TACTCGGTGC TCTGCCTCTT CTCCTACATG GCGGCGGTCA AGGGGAAAGA GTCAGAGGGC
    AAACCCAAGA TGCTGTCCCC TCGTCCACTA GCGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144899
    Clone ID Related Accession (Same CDS sequence) XM_021471970.1
    Accession Version XM_021471970.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4959bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394698.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGACTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGGA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCGGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTTCCCAA GCTCTGCTCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTCCC CAGCCTCAAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGAATTGTGG AGCAAGCACC AGGATAACAG AAAGCAAAAA
    GCTTTGGAAA AAGAACAGAG TGGTTTAAAT AAACGGAAAT CAGGAGCGGA TGGAGTAAAA
    CAACGTGTAG AAGGAGGAGA CACTGAGGAG AAGAAATCAC AGATCAGTGC AGACAGTGGC
    CTCAGTGTGA CATCTGGCTC ACAGAAGAGT GATACGGATT CCCTGGCGAG CTCTGAACCT
    CCTGCTCTGA CCAGAAGCAC CAGCCAGGAC TCAGAGGCCA GCACAGTGGT AAGTAACAGC
    TCAGGTGAGA CGCTCGGTGC AGACAGTGAC CTGAGCAGCA CTGCTGGAGA TGGCCTGACA
    GGAAGACATG GTCAGCATCT CAACCTGTCC CGTGGCACAC TGTCTGACAG TGAGATTGAG
    ACCAACCCTG CAACCAGCTC AGTGTTTGGC AAGACACACA AGCTGAAGCC AGGAGTGAAG
    GAGCCTGTTG GTGTTAATAA AGCAGCACCT GCTCCACCCG TTGAGGATGT TAGCATGAGG
    ATCTATCTGT GTGAGGGGCT TCTGGGCCGG GATAAAAGCT CAGTTTGGGA CCAGTTGGAG
    GATGCTGCAA TGGAAACCTT TTCTCTGAGT AAAGAGCGCT CAACTCTGTG GGATCAGATG
    CAGTTCTGGG AGGACGCCTT CCTGGATGCC GTAATGCTGG AACGGGAAGG AATGGGAATG
    GATCAGGGAC CACAAGAAAT GATTGACAGG TATCTTTCTC TGGGAGATCA TGACCGCAAG
    AGACTGGAGG ATGATGAGGA CAGACTGCTG GCCACGCTTC TGCACAATAT GATTGCTTAC
    ATGCTTATGA TGAAGGTCGG TAAAAACGAC ATCAGGAAGA AAGTACGTCG TCTTATGGGC
    AAATCCCACA TTGGCCTCAC TCACAGTCAG GAGATCAATG AAGTCCTGGA TCGCCTCGCA
    CATTTAAGCG GTCGGGAGCT TTCTATCAGG CCGAGTGGCA GCAGGCATAT TAAAAAACAG
    ACATTTGTTG TGCATGCTGG GACAGATACA ACTGGTGACA TATTCTTCAT GGAGGTGTGT
    GATGATTGCA TCGTCTTGCG CAGTAATATT GGCACAGTCT ATGAGCGCTG GTGGTATGAG
    AAGCTCATAA ATATGACTTA CTGTCCCAAA ACTAAAGTGT TGTGTCTGTG GAGGAGAAAC
    GGCCAGGAGA CGCAGCTAAA CAAGTTTTAC ACTAAGAAGT GTCGGGAATT GTACTACTGT
    GTTAAAGACA GCATGGAGCG TGCTGCTGCT CGACAACAAA GCATCAAACC AGGTCCAGAG
    CTTGGAGGCG AGTTTCCGGT TCAGGACATG AAGACAGGGG AGGGTGGTCT CCTCCAAGTC
    ACACTGGAAG GCATCAACCT CAAATTTATG CACAGCCAGG AGCGGAAGGT TTTCATAGAG
    CTGAATCACA TTAAAAAGTG CAATACAGTG AAAGGAGTCT TTGTCCTGGA GGAGTTTGTT
    CCTGAAACTA AAGAAGTGGT GATCCATAAG TACAAGACTC CTATGGCACA TCAGATCTGT
    TACTCGGTGC TCTGCCTCTT CTCCTACATG GCGGCGGTCA AGGGGAAAGA GTCAGAGGGC
    AAACCCAAGA TGCTGTCCCC TCGTCCACTA GCGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327645.1
    CDS1..4959
    Translation

    Target ORF information:

    RefSeq Version XM_021471970.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X58, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471970.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGACTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGGA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCGGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTTCCCAA GCTCTGCTCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTCCC CAGCCTCAAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGAATTGTGG AGCAAGCACC AGGATAACAG AAAGCAAAAA
    GCTTTGGAAA AAGAACAGAG TGGTTTAAAT AAACGGAAAT CAGGAGCGGA TGGAGTAAAA
    CAACGTGTAG AAGGAGGAGA CACTGAGGAG AAGAAATCAC AGATCAGTGC AGACAGTGGC
    CTCAGTGTGA CATCTGGCTC ACAGAAGAGT GATACGGATT CCCTGGCGAG CTCTGAACCT
    CCTGCTCTGA CCAGAAGCAC CAGCCAGGAC TCAGAGGCCA GCACAGTGGT AAGTAACAGC
    TCAGGTGAGA CGCTCGGTGC AGACAGTGAC CTGAGCAGCA CTGCTGGAGA TGGCCTGACA
    GGAAGACATG GTCAGCATCT CAACCTGTCC CGTGGCACAC TGTCTGACAG TGAGATTGAG
    ACCAACCCTG CAACCAGCTC AGTGTTTGGC AAGACACACA AGCTGAAGCC AGGAGTGAAG
    GAGCCTGTTG GTGTTAATAA AGCAGCACCT GCTCCACCCG TTGAGGATGT TAGCATGAGG
    ATCTATCTGT GTGAGGGGCT TCTGGGCCGG GATAAAAGCT CAGTTTGGGA CCAGTTGGAG
    GATGCTGCAA TGGAAACCTT TTCTCTGAGT AAAGAGCGCT CAACTCTGTG GGATCAGATG
    CAGTTCTGGG AGGACGCCTT CCTGGATGCC GTAATGCTGG AACGGGAAGG AATGGGAATG
    GATCAGGGAC CACAAGAAAT GATTGACAGG TATCTTTCTC TGGGAGATCA TGACCGCAAG
    AGACTGGAGG ATGATGAGGA CAGACTGCTG GCCACGCTTC TGCACAATAT GATTGCTTAC
    ATGCTTATGA TGAAGGTCGG TAAAAACGAC ATCAGGAAGA AAGTACGTCG TCTTATGGGC
    AAATCCCACA TTGGCCTCAC TCACAGTCAG GAGATCAATG AAGTCCTGGA TCGCCTCGCA
    CATTTAAGCG GTCGGGAGCT TTCTATCAGG CCGAGTGGCA GCAGGCATAT TAAAAAACAG
    ACATTTGTTG TGCATGCTGG GACAGATACA ACTGGTGACA TATTCTTCAT GGAGGTGTGT
    GATGATTGCA TCGTCTTGCG CAGTAATATT GGCACAGTCT ATGAGCGCTG GTGGTATGAG
    AAGCTCATAA ATATGACTTA CTGTCCCAAA ACTAAAGTGT TGTGTCTGTG GAGGAGAAAC
    GGCCAGGAGA CGCAGCTAAA CAAGTTTTAC ACTAAGAAGT GTCGGGAATT GTACTACTGT
    GTTAAAGACA GCATGGAGCG TGCTGCTGCT CGACAACAAA GCATCAAACC AGGTCCAGAG
    CTTGGAGGCG AGTTTCCGGT TCAGGACATG AAGACAGGGG AGGGTGGTCT CCTCCAAGTC
    ACACTGGAAG GCATCAACCT CAAATTTATG CACAGCCAGG AGCGGAAGGT TTTCATAGAG
    CTGAATCACA TTAAAAAGTG CAATACAGTG AAAGGAGTCT TTGTCCTGGA GGAGTTTGTT
    CCTGAAACTA AAGAAGTGGT GATCCATAAG TACAAGACTC CTATGGCACA TCAGATCTGT
    TACTCGGTGC TCTGCCTCTT CTCCTACATG GCGGCGGTCA AGGGGAAAGA GTCAGAGGGC
    AAACCCAAGA TGCTGTCCCC TCGTCCACTA GCGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144900
    Clone ID Related Accession (Same CDS sequence) XM_021471971.1
    Accession Version XM_021471971.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4944bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394698.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA CTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGGACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCGGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT TCCCAAGCTC TGCTCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTCCCCAGC CTCAAAGGTG CCCGAGCCCC CAGAGCACTG
    GTAGATCAGA AATCCTCTGT TATTAAGCAC AGCCCTACTG TGAAGAGAGA GTCTCCGTCT
    CCTCAAGGCA GAGCCAACAA TACCAGTGAG AACCAGCAGT TTCTAAAGGA GGTGGTCCAG
    AGCGTGCTGG AGGGGCAGGG TGTGGGCTGG CTTAACATGA AGAAGGTGCG TCGCCTGCTA
    GAGAATGAAC AGCTCAGAGT CTTTGTGCTC AGCAAACTCA ACCGTGCGGT CCAGTCAGAG
    GAGGACGCCC AGCAGGAAGT TATAAGAGAT GTGGAGATAA ATAGAAAGGT CTACAAAGGC
    ATGCTGGATA TTTTGAAGTG CACGGTGTCC AGTTTGGAGC ATTCATACAC TAATGCTGGC
    TTAGGGGGCA TGGCCAGTGT CTTCAGTCTG CTAGAGATAG CACGCACCCA CTACCAAACC
    AAAGATCCAG AGAAGCGGAA ACGGAGCCCC ACCGAAGGGC TTAGCAGTCC AGGCAGTAAG
    GAGAGCCCAT CGGGCCGAAT GGAGAGTGCC AGGGCGGCAG GGGTGCTTCT GGTACCCCGT
    ATCCAATTGC CGCCACCCTC CTCTGGGAAA TCATCACGCC AGTTTGATAC CCGGAGTCTG
    AACGAGGAGA ATTTTATTGC CTCCATTGGA GCGGATGGAG TAAAACAACG TGTAGAAGGA
    GGAGACACTG AGGAGAAGAA ATCACAGATC AGTGCAGACA GTGGCCTCAG TGTGACATCT
    GGCTCACAGA AGAGTGATAC GGATTCCCTG GCGAGCTCTG AACCTCCTGC TCTGACCAGA
    AGCACCAGCC AGGACTCAGA GGCCAGCACA GTGGTAAGTA ACAGCTCAGG TGAGACGCTC
    GGTGCAGACA GTGACCTGAG CAGCACTGCT GGAGATGGCC TGACAGGAAG ACATGGTCAG
    CATCTCAACC TGTCCCGTGG CACACTGTCT GACAGTGAGA TTGAGACCAA CCCTGCAACC
    AGCTCAGTGT TTGGCAAGAC ACACAAGCTG AAGCCAGGAG TGAAGGAGCC TGTTGGTGTT
    AATAAAGCAG CACCTGCTCC ACCCGTTGAG GATGTTAGCA TGAGGATCTA TCTGTGTGAG
    GGGCTTCTGG GCCGGGATAA AAGCTCAGTT TGGGACCAGT TGGAGGATGC TGCAATGGAA
    ACCTTTTCTC TGAGTAAAGA GCGCTCAACT CTGTGGGATC AGATGCAGTT CTGGGAGGAC
    GCCTTCCTGG ATGCCGTAAT GCTGGAACGG GAAGGAATGG GAATGGATCA GGGACCACAA
    GAAATGATTG ACAGGTATCT TTCTCTGGGA GATCATGACC GCAAGAGACT GGAGGATGAT
    GAGGACAGAC TGCTGGCCAC GCTTCTGCAC AATATGATTG CTTACATGCT TATGATGAAG
    GTCGGTAAAA ACGACATCAG GAAGAAAGTA CGTCGTCTTA TGGGCAAATC CCACATTGGC
    CTCACTCACA GTCAGGAGAT CAATGAAGTC CTGGATCGCC TCGCACATTT AAGCGGTCGG
    GAGCTTTCTA TCAGGCCGAG TGGCAGCAGG CATATTAAAA AACAGACATT TGTTGTGCAT
    GCTGGGACAG ATACAACTGG TGACATATTC TTCATGGAGG TGTGTGATGA TTGCATCGTC
    TTGCGCAGTA ATATTGGCAC AGTCTATGAG CGCTGGTGGT ATGAGAAGCT CATAAATATG
    ACTTACTGTC CCAAAACTAA AGTGTTGTGT CTGTGGAGGA GAAACGGCCA GGAGACGCAG
    CTAAACAAGT TTTACACTAA GAAGTGTCGG GAATTGTACT ACTGTGTTAA AGACAGCATG
    GAGCGTGCTG CTGCTCGACA ACAAAGCATC AAACCAGGTC CAGAGCTTGG AGGCGAGTTT
    CCGGTTCAGG ACATGAAGAC AGGGGAGGGT GGTCTCCTCC AAGTCACACT GGAAGGCATC
    AACCTCAAAT TTATGCACAG CCAGGAGCGG AAGGTTTTCA TAGAGCTGAA TCACATTAAA
    AAGTGCAATA CAGTGAAAGG AGTCTTTGTC CTGGAGGAGT TTGTTCCTGA AACTAAAGAA
    GTGGTGATCC ATAAGTACAA GACTCCTATG GCACATCAGA TCTGTTACTC GGTGCTCTGC
    CTCTTCTCCT ACATGGCGGC GGTCAAGGGG AAAGAGTCAG AGGGCAAACC CAAGATGCTG
    TCCCCTCGTC CACTAGCGAG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327646.1
    CDS169..5112
    Translation

    Target ORF information:

    RefSeq Version XM_021471971.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X59, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471971.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA CTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGGACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCGGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT TCCCAAGCTC TGCTCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTCCCCAGC CTCAAAGGTG CCCGAGCCCC CAGAGCACTG
    GTAGATCAGA AATCCTCTGT TATTAAGCAC AGCCCTACTG TGAAGAGAGA GTCTCCGTCT
    CCTCAAGGCA GAGCCAACAA TACCAGTGAG AACCAGCAGT TTCTAAAGGA GGTGGTCCAG
    AGCGTGCTGG AGGGGCAGGG TGTGGGCTGG CTTAACATGA AGAAGGTGCG TCGCCTGCTA
    GAGAATGAAC AGCTCAGAGT CTTTGTGCTC AGCAAACTCA ACCGTGCGGT CCAGTCAGAG
    GAGGACGCCC AGCAGGAAGT TATAAGAGAT GTGGAGATAA ATAGAAAGGT CTACAAAGGC
    ATGCTGGATA TTTTGAAGTG CACGGTGTCC AGTTTGGAGC ATTCATACAC TAATGCTGGC
    TTAGGGGGCA TGGCCAGTGT CTTCAGTCTG CTAGAGATAG CACGCACCCA CTACCAAACC
    AAAGATCCAG AGAAGCGGAA ACGGAGCCCC ACCGAAGGGC TTAGCAGTCC AGGCAGTAAG
    GAGAGCCCAT CGGGCCGAAT GGAGAGTGCC AGGGCGGCAG GGGTGCTTCT GGTACCCCGT
    ATCCAATTGC CGCCACCCTC CTCTGGGAAA TCATCACGCC AGTTTGATAC CCGGAGTCTG
    AACGAGGAGA ATTTTATTGC CTCCATTGGA GCGGATGGAG TAAAACAACG TGTAGAAGGA
    GGAGACACTG AGGAGAAGAA ATCACAGATC AGTGCAGACA GTGGCCTCAG TGTGACATCT
    GGCTCACAGA AGAGTGATAC GGATTCCCTG GCGAGCTCTG AACCTCCTGC TCTGACCAGA
    AGCACCAGCC AGGACTCAGA GGCCAGCACA GTGGTAAGTA ACAGCTCAGG TGAGACGCTC
    GGTGCAGACA GTGACCTGAG CAGCACTGCT GGAGATGGCC TGACAGGAAG ACATGGTCAG
    CATCTCAACC TGTCCCGTGG CACACTGTCT GACAGTGAGA TTGAGACCAA CCCTGCAACC
    AGCTCAGTGT TTGGCAAGAC ACACAAGCTG AAGCCAGGAG TGAAGGAGCC TGTTGGTGTT
    AATAAAGCAG CACCTGCTCC ACCCGTTGAG GATGTTAGCA TGAGGATCTA TCTGTGTGAG
    GGGCTTCTGG GCCGGGATAA AAGCTCAGTT TGGGACCAGT TGGAGGATGC TGCAATGGAA
    ACCTTTTCTC TGAGTAAAGA GCGCTCAACT CTGTGGGATC AGATGCAGTT CTGGGAGGAC
    GCCTTCCTGG ATGCCGTAAT GCTGGAACGG GAAGGAATGG GAATGGATCA GGGACCACAA
    GAAATGATTG ACAGGTATCT TTCTCTGGGA GATCATGACC GCAAGAGACT GGAGGATGAT
    GAGGACAGAC TGCTGGCCAC GCTTCTGCAC AATATGATTG CTTACATGCT TATGATGAAG
    GTCGGTAAAA ACGACATCAG GAAGAAAGTA CGTCGTCTTA TGGGCAAATC CCACATTGGC
    CTCACTCACA GTCAGGAGAT CAATGAAGTC CTGGATCGCC TCGCACATTT AAGCGGTCGG
    GAGCTTTCTA TCAGGCCGAG TGGCAGCAGG CATATTAAAA AACAGACATT TGTTGTGCAT
    GCTGGGACAG ATACAACTGG TGACATATTC TTCATGGAGG TGTGTGATGA TTGCATCGTC
    TTGCGCAGTA ATATTGGCAC AGTCTATGAG CGCTGGTGGT ATGAGAAGCT CATAAATATG
    ACTTACTGTC CCAAAACTAA AGTGTTGTGT CTGTGGAGGA GAAACGGCCA GGAGACGCAG
    CTAAACAAGT TTTACACTAA GAAGTGTCGG GAATTGTACT ACTGTGTTAA AGACAGCATG
    GAGCGTGCTG CTGCTCGACA ACAAAGCATC AAACCAGGTC CAGAGCTTGG AGGCGAGTTT
    CCGGTTCAGG ACATGAAGAC AGGGGAGGGT GGTCTCCTCC AAGTCACACT GGAAGGCATC
    AACCTCAAAT TTATGCACAG CCAGGAGCGG AAGGTTTTCA TAGAGCTGAA TCACATTAAA
    AAGTGCAATA CAGTGAAAGG AGTCTTTGTC CTGGAGGAGT TTGTTCCTGA AACTAAAGAA
    GTGGTGATCC ATAAGTACAA GACTCCTATG GCACATCAGA TCTGTTACTC GGTGCTCTGC
    CTCTTCTCCT ACATGGCGGC GGTCAAGGGG AAAGAGTCAG AGGGCAAACC CAAGATGCTG
    TCCCCTCGTC CACTAGCGAG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144902
    Clone ID Related Accession (Same CDS sequence) XM_021471973.1
    Accession Version XM_021471973.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4935bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394698.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA CTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGGACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCGGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT TCCCAAGCTC TGCTCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTCCCCAGC CTCAAAGAAT ATTTTAATTT TGATTTAGCA
    GAGGACACAG ATCAAGACGA TGCTGATGAC AGTCCTGAAA GTCCTGTATT TGGGCTAAAT
    TCTCTAATGG AGATTGTAAC AGAGATCGGC CCGGGGAGCG GAGAAGGTGC CCGAGCCCCC
    AGAGCACTGG TAGATCAGAA ATCCTCTGTT ATTAAGCACA GCCCTACTGT GAAGAGAGAG
    TCTCCGTCTC CTCAAGGCAG AGCCAACAAT ACCAGTGAGA ACCAGCAGTT TCTAAAGGAG
    GTGGTCCAGA GCGTGCTGGA GGGGCAGGGT GTGGGCTGGC TTAACATGAA GAAGGTGCGT
    CGCCTGCTAG AGAATGAACA GCTCAGAGTC TTTGTGCTCA GCAAACTCAA CCGTGCGGTC
    CAGTCAGAGG AGGACGCCCA GCAGGAAGTT ATAAGAGATG TGGAGATAAA TAGAAAGGTC
    TACAAAGGCA TGCTGGATAT TTTGAAGTGC ACGGTGTCCA GTTTGGAGCA TTCATACACT
    AATGCTGGCT TAGGGGGCAT GGCCAGTGTC TTCAGTCTGC TAGAGATAGC ACGCACCCAC
    TACCAAACCA AAGATCCAGA GAAGCGGAAA CGGAGCCCCA CCGAAGGGCT TAGCAGTCCA
    GGCAGTAAGG AGAGCCCATC GGGCCGAATG GAGAGTGCCA GGGCGGCAGG GGTGCTTCTG
    GTACCCCGTA TCCAATTGCC GCCACCCTCC TCTGGGAAAT CATCACGCCA GTTTGATACC
    CGGAGTCTGA ACGAGGAGAA TTTTATTGCC TCCATTGAAT TGTGGAGCAA GCACCAGGAT
    AACAGAAAGC AAAAAGCTTT GGAAAAAGAA CAGAGTGGTT TAAATAAACG GAAATCAGGA
    GCGGATGGAG TAAAACAACG TGTAGAAGGA GGAGACACTG AGGAGAAGAA ATCACAGATC
    AGTGCAGACA GTGGCCTCAG TGTGACATCT GGCTCACAGA AGAGTGATAC GGATTCCCTG
    GCGAGCTCTG AACCTCCTGC TCTGACCAGA AGCACCAGCC AGGACTCAGA GGCCAGCACA
    GTAAGTAACA GCTCAGGTGA GACGCTCGGT GCAGACAGTG ACCTGAGCAG CACTGCTGGA
    GATGGCCTGA CAGGAAGACA TGGTCAGCAT CTCAACCTGT CCCGTGGCAC ACTGTCTGAC
    AGTGAGATTG AGACCAACCC TGCAACCAGC TCAGTGTTTG GCAAGACACA CAAGCTGAAG
    CCAGGAGTGA AGGAGCCTGT TGGTGTTAAT AAAGCAGCAC CTGCTCCACC CGTTGAGGAT
    GTTAGCATGA GGATCTATCT GTGTGAGGGG CTTCTGGGCC GGGATAAAAG CTCAGTTTGG
    GACCAGTTGG AGGATGCTGC AATGGAAACC TTTTCTCTGA GTAAAGAGCG CTCAACTCTG
    TGGGATCAGA TGCAGTTCTG GGAGGACGCC TTCCTGGATG CCGTAATGCT GGAACGGGAA
    GGAATGGGAA TGGATCAGGG ACCACAAGAA ATGATTGACA GGTATCTTTC TCTGGGAGAT
    CATGACCGCA AGAGACTGGA GGATGATGAG GACAGACTGC TGGCCACGCT TCTGCACAAT
    ATGATTGCTT ACATGCTTAT GATGAAGGTC GGTAAAAACG ACATCAGGAA GAAAGTACGT
    CGTCTTATGG GCAAATCCCA CATTGGCCTC ACTCACAGTC AGGAGATCAA TGAAGTCCTG
    GATCGCCTCG CACATTTAAG CGGTCGGGAG CTTTCTATCA GGCCGAGTGG CAGCAGGCAT
    ATTAAAAAAC AGACATTTGT TGTGCATGCT GGGACAGATA CAACTGGTGA CATATTCTTC
    ATGGAGGTGT GTGATGATTG CATCGTCTTG CGCAGTAATA TTGGCACAGT CTATGAGCGC
    TGGTGGTATG AGAAGCTCAT AAATATGACT TACTGTCCCA AAACTAAAGT GTTGTGTCTG
    TGGAGGAGAA ACGGCCAGGA GACGCAGCTA AACAAGTTTT ACACTAAGAA GTGTCGGGAA
    TTGTACTACT GTGTTAAAGA CAGCATGGAG CGTGCTGCTG CTCGACAACA AAGCATCAAA
    CCAGGTCCAG AGCTTGGAGG CGAGTTTCCG GTTCAGGACA TGAAGACAGG GGAGGGTGGT
    CTCCTCCAAG TCACACTGGA AGGCATCAAC CTCAAATTTA TGCACAGCCA GTTCCTGAAA
    CTAAAGAAGT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327648.1
    CDS170..5104
    Translation

    Target ORF information:

    RefSeq Version XM_021471973.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X61, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471973.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA CTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGGACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCGGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT TCCCAAGCTC TGCTCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTCCCCAGC CTCAAAGAAT ATTTTAATTT TGATTTAGCA
    GAGGACACAG ATCAAGACGA TGCTGATGAC AGTCCTGAAA GTCCTGTATT TGGGCTAAAT
    TCTCTAATGG AGATTGTAAC AGAGATCGGC CCGGGGAGCG GAGAAGGTGC CCGAGCCCCC
    AGAGCACTGG TAGATCAGAA ATCCTCTGTT ATTAAGCACA GCCCTACTGT GAAGAGAGAG
    TCTCCGTCTC CTCAAGGCAG AGCCAACAAT ACCAGTGAGA ACCAGCAGTT TCTAAAGGAG
    GTGGTCCAGA GCGTGCTGGA GGGGCAGGGT GTGGGCTGGC TTAACATGAA GAAGGTGCGT
    CGCCTGCTAG AGAATGAACA GCTCAGAGTC TTTGTGCTCA GCAAACTCAA CCGTGCGGTC
    CAGTCAGAGG AGGACGCCCA GCAGGAAGTT ATAAGAGATG TGGAGATAAA TAGAAAGGTC
    TACAAAGGCA TGCTGGATAT TTTGAAGTGC ACGGTGTCCA GTTTGGAGCA TTCATACACT
    AATGCTGGCT TAGGGGGCAT GGCCAGTGTC TTCAGTCTGC TAGAGATAGC ACGCACCCAC
    TACCAAACCA AAGATCCAGA GAAGCGGAAA CGGAGCCCCA CCGAAGGGCT TAGCAGTCCA
    GGCAGTAAGG AGAGCCCATC GGGCCGAATG GAGAGTGCCA GGGCGGCAGG GGTGCTTCTG
    GTACCCCGTA TCCAATTGCC GCCACCCTCC TCTGGGAAAT CATCACGCCA GTTTGATACC
    CGGAGTCTGA ACGAGGAGAA TTTTATTGCC TCCATTGAAT TGTGGAGCAA GCACCAGGAT
    AACAGAAAGC AAAAAGCTTT GGAAAAAGAA CAGAGTGGTT TAAATAAACG GAAATCAGGA
    GCGGATGGAG TAAAACAACG TGTAGAAGGA GGAGACACTG AGGAGAAGAA ATCACAGATC
    AGTGCAGACA GTGGCCTCAG TGTGACATCT GGCTCACAGA AGAGTGATAC GGATTCCCTG
    GCGAGCTCTG AACCTCCTGC TCTGACCAGA AGCACCAGCC AGGACTCAGA GGCCAGCACA
    GTAAGTAACA GCTCAGGTGA GACGCTCGGT GCAGACAGTG ACCTGAGCAG CACTGCTGGA
    GATGGCCTGA CAGGAAGACA TGGTCAGCAT CTCAACCTGT CCCGTGGCAC ACTGTCTGAC
    AGTGAGATTG AGACCAACCC TGCAACCAGC TCAGTGTTTG GCAAGACACA CAAGCTGAAG
    CCAGGAGTGA AGGAGCCTGT TGGTGTTAAT AAAGCAGCAC CTGCTCCACC CGTTGAGGAT
    GTTAGCATGA GGATCTATCT GTGTGAGGGG CTTCTGGGCC GGGATAAAAG CTCAGTTTGG
    GACCAGTTGG AGGATGCTGC AATGGAAACC TTTTCTCTGA GTAAAGAGCG CTCAACTCTG
    TGGGATCAGA TGCAGTTCTG GGAGGACGCC TTCCTGGATG CCGTAATGCT GGAACGGGAA
    GGAATGGGAA TGGATCAGGG ACCACAAGAA ATGATTGACA GGTATCTTTC TCTGGGAGAT
    CATGACCGCA AGAGACTGGA GGATGATGAG GACAGACTGC TGGCCACGCT TCTGCACAAT
    ATGATTGCTT ACATGCTTAT GATGAAGGTC GGTAAAAACG ACATCAGGAA GAAAGTACGT
    CGTCTTATGG GCAAATCCCA CATTGGCCTC ACTCACAGTC AGGAGATCAA TGAAGTCCTG
    GATCGCCTCG CACATTTAAG CGGTCGGGAG CTTTCTATCA GGCCGAGTGG CAGCAGGCAT
    ATTAAAAAAC AGACATTTGT TGTGCATGCT GGGACAGATA CAACTGGTGA CATATTCTTC
    ATGGAGGTGT GTGATGATTG CATCGTCTTG CGCAGTAATA TTGGCACAGT CTATGAGCGC
    TGGTGGTATG AGAAGCTCAT AAATATGACT TACTGTCCCA AAACTAAAGT GTTGTGTCTG
    TGGAGGAGAA ACGGCCAGGA GACGCAGCTA AACAAGTTTT ACACTAAGAA GTGTCGGGAA
    TTGTACTACT GTGTTAAAGA CAGCATGGAG CGTGCTGCTG CTCGACAACA AAGCATCAAA
    CCAGGTCCAG AGCTTGGAGG CGAGTTTCCG GTTCAGGACA TGAAGACAGG GGAGGGTGGT
    CTCCTCCAAG TCACACTGGA AGGCATCAAC CTCAAATTTA TGCACAGCCA GTTCCTGAAA
    CTAAAGAAGT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144904
    Clone ID Related Accession (Same CDS sequence) XM_021471975.1
    Accession Version XM_021471975.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4881bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X23
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394698.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA CTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGGACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCGGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT TCCCAAGCTC TGCTCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTCCCCAGC CTCAAAGGTG CCCGAGCCCC CAGAGCACTG
    GTAGATCAGA AATCCTCTGT TATTAAGCAC AGCCCTACTG TGAAGAGAGA GTCTCCGTCT
    CCTCAAGGCA GAGCCAACAA TACCAGTGAG AACCAGCAGT TTCTAAAGGA GGTGGTCCAG
    AGCGTGCTGG AGGGGCAGGG TGTGGGCTGG CTTAACATGA AGAAGGTGCG TCGCCTGCTA
    GAGAATGAAC AGCTCAGAGT CTTTGTGCTC AGCAAACTCA ACCGTGCGGT CCAGTCAGAG
    GAGGACGCCC AGCAGGAAGT TATAAGAGAT GTGGAGATAA ATAGAAAGGT CTACAAAGGC
    ATGCTGGATA TTTTGAAGTG CACGGTGTCC AGTTTGGAGC ATTCATACAC TAATGCTGGC
    TTAGGGGGCA TGGCCAGTGT CTTCAGTCTG CTAGAGATAG CACGCACCCA CTACCAAACC
    AAAGATCCAG AGAAGCGGAA ACGGAGCCCC ACCGAAGGGC TTAGCAGTCC AGGCAGTAAG
    GAGAGCCCAT CGGGCCGAAT GGAGAGTGCC AGGGCGGCAG GGGTGCTTCT GGTACCCCGT
    ATCCAATTGC CGCCACCCTC CTCTGGGAAA TCATCACGCC AGTTTGATAC CCGGAGTCTG
    AACGAGGAGA ATTTTATTGC CTCCATTGGA GCGGATGGAG TAAAACAACG TGTAGAAGGA
    GGAGACACTG AGGAGAAGAA ATCACAGATC AGTGCAGACA GTGGCCTCAG TGTGACATCT
    GGCTCACAGA AGAGTGATAC GGATTCCCTG GCGAGCTCTG AACCTCCTGC TCTGACCAGA
    AGCACCAGCC AGGACTCAGA GGCCAGCACA GTGGTAAGTA ACAGCTCAGG TGAGACGCTC
    GGTGCAGACA GTGACCTGAG CAGCACTGCT GGAGATGGCC TGACAGGAAG ACATGGTCAG
    CATCTCAACC TGTCCCGTGG CACACTGTCT GACAGTGAGA TTGAGACCAA CCCTGCAACC
    AGCTCAGTGT TTGGCAAGAC ACACAAGCTG AAGCCAGGAG TGAAGGAGCC TGTTGGTGTT
    AATAAAGCAG CACCTGCTCC ACCCGTTGAG GATGTTAGCA TGAGGATCTA TCTGTGTGAG
    GGGCTTCTGG GTAAAGAGCG CTCAACTCTG TGGGATCAGA TGCAGTTCTG GGAGGACGCC
    TTCCTGGATG CCGTAATGCT GGAACGGGAA GGAATGGGAA TGGATCAGGG ACCACAAGAA
    ATGATTGACA GGTATCTTTC TCTGGGAGAT CATGACCGCA AGAGACTGGA GGATGATGAG
    GACAGACTGC TGGCCACGCT TCTGCACAAT ATGATTGCTT ACATGCTTAT GATGAAGGTC
    GGTAAAAACG ACATCAGGAA GAAAGTACGT CGTCTTATGG GCAAATCCCA CATTGGCCTC
    ACTCACAGTC AGGAGATCAA TGAAGTCCTG GATCGCCTCG CACATTTAAG CGGTCGGGAG
    CTTTCTATCA GGCCGAGTGG CAGCAGGCAT ATTAAAAAAC AGACATTTGT TGTGCATGCT
    GGGACAGATA CAACTGGTGA CATATTCTTC ATGGAGGTGT GTGATGATTG CATCGTCTTG
    CGCAGTAATA TTGGCACAGT CTATGAGCGC TGGTGGTATG AGAAGCTCAT AAATATGACT
    TACTGTCCCA AAACTAAAGT GTTGTGTCTG TGGAGGAGAA ACGGCCAGGA GACGCAGCTA
    AACAAGTTTT ACACTAAGAA GTGTCGGGAA TTGTACTACT GTGTTAAAGA CAGCATGGAG
    CGTGCTGCTG CTCGACAACA AAGCATCAAA CCAGGTCCAG AGCTTGGAGG CGAGTTTCCG
    GTTCAGGACA TGAAGACAGG GGAGGGTGGT CTCCTCCAAG TCACACTGGA AGGCATCAAC
    CTCAAATTTA TGCACAGCCA GGAGCGGAAG GTTTTCATAG AGCTGAATCA CATTAAAAAG
    TGCAATACAG TGAAAGGAGT CTTTGTCCTG GAGGAGTTTG TTCCTGAAAC TAAAGAAGTG
    GTGATCCATA AGTACAAGAC TCCTATGGCA CATCAGATCT GTTACTCGGT GCTCTGCCTC
    TTCTCCTACA TGGCGGCGGT CAAGGGGAAA GAGTCAGAGG GCAAACCCAA GATGCTGTCC
    CCTCGTCCAC TAGCGAGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327650.1
    CDS169..5049
    Translation

    Target ORF information:

    RefSeq Version XM_021471975.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X63, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471975.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA CTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGGACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCGGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT TCCCAAGCTC TGCTCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTCCCCAGC CTCAAAGGTG CCCGAGCCCC CAGAGCACTG
    GTAGATCAGA AATCCTCTGT TATTAAGCAC AGCCCTACTG TGAAGAGAGA GTCTCCGTCT
    CCTCAAGGCA GAGCCAACAA TACCAGTGAG AACCAGCAGT TTCTAAAGGA GGTGGTCCAG
    AGCGTGCTGG AGGGGCAGGG TGTGGGCTGG CTTAACATGA AGAAGGTGCG TCGCCTGCTA
    GAGAATGAAC AGCTCAGAGT CTTTGTGCTC AGCAAACTCA ACCGTGCGGT CCAGTCAGAG
    GAGGACGCCC AGCAGGAAGT TATAAGAGAT GTGGAGATAA ATAGAAAGGT CTACAAAGGC
    ATGCTGGATA TTTTGAAGTG CACGGTGTCC AGTTTGGAGC ATTCATACAC TAATGCTGGC
    TTAGGGGGCA TGGCCAGTGT CTTCAGTCTG CTAGAGATAG CACGCACCCA CTACCAAACC
    AAAGATCCAG AGAAGCGGAA ACGGAGCCCC ACCGAAGGGC TTAGCAGTCC AGGCAGTAAG
    GAGAGCCCAT CGGGCCGAAT GGAGAGTGCC AGGGCGGCAG GGGTGCTTCT GGTACCCCGT
    ATCCAATTGC CGCCACCCTC CTCTGGGAAA TCATCACGCC AGTTTGATAC CCGGAGTCTG
    AACGAGGAGA ATTTTATTGC CTCCATTGGA GCGGATGGAG TAAAACAACG TGTAGAAGGA
    GGAGACACTG AGGAGAAGAA ATCACAGATC AGTGCAGACA GTGGCCTCAG TGTGACATCT
    GGCTCACAGA AGAGTGATAC GGATTCCCTG GCGAGCTCTG AACCTCCTGC TCTGACCAGA
    AGCACCAGCC AGGACTCAGA GGCCAGCACA GTGGTAAGTA ACAGCTCAGG TGAGACGCTC
    GGTGCAGACA GTGACCTGAG CAGCACTGCT GGAGATGGCC TGACAGGAAG ACATGGTCAG
    CATCTCAACC TGTCCCGTGG CACACTGTCT GACAGTGAGA TTGAGACCAA CCCTGCAACC
    AGCTCAGTGT TTGGCAAGAC ACACAAGCTG AAGCCAGGAG TGAAGGAGCC TGTTGGTGTT
    AATAAAGCAG CACCTGCTCC ACCCGTTGAG GATGTTAGCA TGAGGATCTA TCTGTGTGAG
    GGGCTTCTGG GTAAAGAGCG CTCAACTCTG TGGGATCAGA TGCAGTTCTG GGAGGACGCC
    TTCCTGGATG CCGTAATGCT GGAACGGGAA GGAATGGGAA TGGATCAGGG ACCACAAGAA
    ATGATTGACA GGTATCTTTC TCTGGGAGAT CATGACCGCA AGAGACTGGA GGATGATGAG
    GACAGACTGC TGGCCACGCT TCTGCACAAT ATGATTGCTT ACATGCTTAT GATGAAGGTC
    GGTAAAAACG ACATCAGGAA GAAAGTACGT CGTCTTATGG GCAAATCCCA CATTGGCCTC
    ACTCACAGTC AGGAGATCAA TGAAGTCCTG GATCGCCTCG CACATTTAAG CGGTCGGGAG
    CTTTCTATCA GGCCGAGTGG CAGCAGGCAT ATTAAAAAAC AGACATTTGT TGTGCATGCT
    GGGACAGATA CAACTGGTGA CATATTCTTC ATGGAGGTGT GTGATGATTG CATCGTCTTG
    CGCAGTAATA TTGGCACAGT CTATGAGCGC TGGTGGTATG AGAAGCTCAT AAATATGACT
    TACTGTCCCA AAACTAAAGT GTTGTGTCTG TGGAGGAGAA ACGGCCAGGA GACGCAGCTA
    AACAAGTTTT ACACTAAGAA GTGTCGGGAA TTGTACTACT GTGTTAAAGA CAGCATGGAG
    CGTGCTGCTG CTCGACAACA AAGCATCAAA CCAGGTCCAG AGCTTGGAGG CGAGTTTCCG
    GTTCAGGACA TGAAGACAGG GGAGGGTGGT CTCCTCCAAG TCACACTGGA AGGCATCAAC
    CTCAAATTTA TGCACAGCCA GGAGCGGAAG GTTTTCATAG AGCTGAATCA CATTAAAAAG
    TGCAATACAG TGAAAGGAGT CTTTGTCCTG GAGGAGTTTG TTCCTGAAAC TAAAGAAGTG
    GTGATCCATA AGTACAAGAC TCCTATGGCA CATCAGATCT GTTACTCGGT GCTCTGCCTC
    TTCTCCTACA TGGCGGCGGT CAAGGGGAAA GAGTCAGAGG GCAAACCCAA GATGCTGTCC
    CCTCGTCCAC TAGCGAGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144905
    Clone ID Related Accession (Same CDS sequence) XM_021471976.1
    Accession Version XM_021471976.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4875bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X24
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394698.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGACTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGGA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCGGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTTCCCAA GCTCTGCTCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTCCC CAGCCTCAAA GTATTTGGGC TAAATTCTCT AATGGAGATT
    GTAACAGAGA TCGGCCCGGG GAGCGGAGAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGGAGCGGAT GGAGTAAAAC AACGTGTAGA AGGAGGAGAC
    ACTGAGGAGA AGAAATCACA GATCAGTGCA GACAGTGGCC TCAGTGTGAC ATCTGGCTCA
    CAGAAGAGTG ATACGGATTC CCTGGCGAGC TCTGAACCTC CTGCTCTGAC CAGAAGCACC
    AGCCAGGACT CAGAGGCCAG CACAGTGGTA AGTAACAGCT CAGGTGAGAC GCTCGGTGCA
    GACAGTGACC TGAGCAGCAC TGCTGGAGAT GGCCTGACAG GAAGACATGG TCAGCATCTC
    AACCTGTCCC GTGGCACACT GTCTGACAGT GAGATTGAGA CCAACCCTGC AACCAGCTCA
    GTGTTTGGCA AGACACACAA GCTGAAGCCA GGAGTGAAGG AGCCTGTTGG TGTTAATAAA
    GCAGCACCTG CTCCACCCGT TGAGGATGTT AGCATGAGGA TCTATCTGTG TGAGGGGCTT
    CTGGGTAAAG AGCGCTCAAC TCTGTGGGAT CAGATGCAGT TCTGGGAGGA CGCCTTCCTG
    GATGCCGTAA TGCTGGAACG GGAAGGAATG GGAATGGATC AGGGACCACA AGAAATGATT
    GACAGGTATC TTTCTCTGGG AGATCATGAC CGCAAGAGAC TGGAGGATGA TGAGGACAGA
    CTGCTGGCCA CGCTTCTGCA CAATATGATT GCTTACATGC TTATGATGAA GGTCGGTAAA
    AACGACATCA GGAAGAAAGT ACGTCGTCTT ATGGGCAAAT CCCACATTGG CCTCACTCAC
    AGTCAGGAGA TCAATGAAGT CCTGGATCGC CTCGCACATT TAAGCGGTCG GGAGCTTTCT
    ATCAGGCCGA GTGGCAGCAG GCATATTAAA AAACAGACAT TTGTTGTGCA TGCTGGGACA
    GATACAACTG GTGACATATT CTTCATGGAG GTGTGTGATG ATTGCATCGT CTTGCGCAGT
    AATATTGGCA CAGTCTATGA GCGCTGGTGG TATGAGAAGC TCATAAATAT GACTTACTGT
    CCCAAAACTA AAGTGTTGTG TCTGTGGAGG AGAAACGGCC AGGAGACGCA GCTAAACAAG
    TTTTACACTA AGAAGTGTCG GGAATTGTAC TACTGTGTTA AAGACAGCAT GGAGCGTGCT
    GCTGCTCGAC AACAAAGCAT CAAACCAGGT CCAGAGCTTG GAGGCGAGTT TCCGGTTCAG
    GACATGAAGA CAGGGGAGGG TGGTCTCCTC CAAGTCACAC TGGAAGGCAT CAACCTCAAA
    TTTATGCACA GCCAGGAGCG GAAGGTTTTC ATAGAGCTGA ATCACATTAA AAAGTGCAAT
    ACAGTGAAAG GAGTCTTTGT CCTGGAGGAG TTTGTTCCTG AAACTAAAGA AGTGGTGATC
    CATAAGTACA AGACTCCTAT GGCACATCAG ATCTGTTACT CGGTGCTCTG CCTCTTCTCC
    TACATGGCGG CGGTCAAGGG GAAAGAGTCA GAGGGCAAAC CCAAGATGCT GTCCCCTCGT
    CCACTAGCGA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327651.1
    CDS1..4875
    Translation

    Target ORF information:

    RefSeq Version XM_021471976.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471976.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGACTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGGA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCGGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTTCCCAA GCTCTGCTCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTCCC CAGCCTCAAA GTATTTGGGC TAAATTCTCT AATGGAGATT
    GTAACAGAGA TCGGCCCGGG GAGCGGAGAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGGAGCGGAT GGAGTAAAAC AACGTGTAGA AGGAGGAGAC
    ACTGAGGAGA AGAAATCACA GATCAGTGCA GACAGTGGCC TCAGTGTGAC ATCTGGCTCA
    CAGAAGAGTG ATACGGATTC CCTGGCGAGC TCTGAACCTC CTGCTCTGAC CAGAAGCACC
    AGCCAGGACT CAGAGGCCAG CACAGTGGTA AGTAACAGCT CAGGTGAGAC GCTCGGTGCA
    GACAGTGACC TGAGCAGCAC TGCTGGAGAT GGCCTGACAG GAAGACATGG TCAGCATCTC
    AACCTGTCCC GTGGCACACT GTCTGACAGT GAGATTGAGA CCAACCCTGC AACCAGCTCA
    GTGTTTGGCA AGACACACAA GCTGAAGCCA GGAGTGAAGG AGCCTGTTGG TGTTAATAAA
    GCAGCACCTG CTCCACCCGT TGAGGATGTT AGCATGAGGA TCTATCTGTG TGAGGGGCTT
    CTGGGTAAAG AGCGCTCAAC TCTGTGGGAT CAGATGCAGT TCTGGGAGGA CGCCTTCCTG
    GATGCCGTAA TGCTGGAACG GGAAGGAATG GGAATGGATC AGGGACCACA AGAAATGATT
    GACAGGTATC TTTCTCTGGG AGATCATGAC CGCAAGAGAC TGGAGGATGA TGAGGACAGA
    CTGCTGGCCA CGCTTCTGCA CAATATGATT GCTTACATGC TTATGATGAA GGTCGGTAAA
    AACGACATCA GGAAGAAAGT ACGTCGTCTT ATGGGCAAAT CCCACATTGG CCTCACTCAC
    AGTCAGGAGA TCAATGAAGT CCTGGATCGC CTCGCACATT TAAGCGGTCG GGAGCTTTCT
    ATCAGGCCGA GTGGCAGCAG GCATATTAAA AAACAGACAT TTGTTGTGCA TGCTGGGACA
    GATACAACTG GTGACATATT CTTCATGGAG GTGTGTGATG ATTGCATCGT CTTGCGCAGT
    AATATTGGCA CAGTCTATGA GCGCTGGTGG TATGAGAAGC TCATAAATAT GACTTACTGT
    CCCAAAACTA AAGTGTTGTG TCTGTGGAGG AGAAACGGCC AGGAGACGCA GCTAAACAAG
    TTTTACACTA AGAAGTGTCG GGAATTGTAC TACTGTGTTA AAGACAGCAT GGAGCGTGCT
    GCTGCTCGAC AACAAAGCAT CAAACCAGGT CCAGAGCTTG GAGGCGAGTT TCCGGTTCAG
    GACATGAAGA CAGGGGAGGG TGGTCTCCTC CAAGTCACAC TGGAAGGCAT CAACCTCAAA
    TTTATGCACA GCCAGGAGCG GAAGGTTTTC ATAGAGCTGA ATCACATTAA AAAGTGCAAT
    ACAGTGAAAG GAGTCTTTGT CCTGGAGGAG TTTGTTCCTG AAACTAAAGA AGTGGTGATC
    CATAAGTACA AGACTCCTAT GGCACATCAG ATCTGTTACT CGGTGCTCTG CCTCTTCTCC
    TACATGGCGG CGGTCAAGGG GAAAGAGTCA GAGGGCAAAC CCAAGATGCT GTCCCCTCGT
    CCACTAGCGA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144906
    Clone ID Related Accession (Same CDS sequence) XM_021471977.1
    Accession Version XM_021471977.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4872bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X25
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394698.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA CTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGGACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCGGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT TCCCAAGCTC TGCTCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTCCCCAGC CTCAAAGGTG CCCGAGCCCC CAGAGCACTG
    GTAGATCAGA AATCCTCTGT TATTAAGCAC AGCCCTACTG TGAAGAGAGA GTCTCCGTCT
    CCTCAAGGCA GAGCCAACAA TACCAGTGAG AACCAGCAGT TTCTAAAGGA GGTGGTCCAG
    AGCGTGCTGG AGGGGCAGGG TGTGGGCTGG CTTAACATGA AGAAGGTGCG TCGCCTGCTA
    GAGAATGAAC AGCTCAGAGT CTTTGTGCTC AGCAAACTCA ACCGTGCGGT CCAGTCAGAG
    GAGGACGCCC AGCAGGAAGT TATAAGAGAT GTGGAGATAA ATAGAAAGGT CTACAAAGGC
    ATGCTGGATA TTTTGAAGTG CACGGTGTCC AGTTTGGAGC ATTCATACAC TAATGCTGGC
    TTAGGGGGCA TGGCCAGTGT CTTCAGTCTG CTAGAGATAG CACGCACCCA CTACCAAACC
    AAAGATCCAG AGAAGCGGAA ACGGAGCCCC ACCGAAGGGC TTAGCAGTCC AGGCAGTAAG
    GAGAGCCCAT CGGGCCGAAT GGAGAGTGCC AGGGCGGCAG GGGTGCTTCT GGTACCCCGT
    ATCCAATTGC CGCCACCCTC CTCTGGGAAA TCATCACGCC AGTTTGATAC CCGGAGTCTG
    AACGAGGAGA ATTTTATTGC CTCCATTGGA GCGGATGGAG TAAAACAACG TGTAGAAGGA
    GGAGACACTG AGGAGAAGAA ATCACAGATC AGTGCAGACA GTGGCCTCAG TGTGACATCT
    GGCTCACAGA AGAGTGATAC GGATTCCCTG GCGAGCTCTG AACCTCCTGC TCTGACCAGA
    AGCACCAGCC AGGACTCAGA GGCCAGCACA GTGGTAAGTA ACAGCTCAGG TGAGACGCTC
    GGTGCAGACA GTGACCTGAG CAGCACTGCT GGAGATGGCC TGACAGGAAG ACATGGTCAG
    CATCTCAACC TGTCCCGTGG CACACTGTCT GACAGTGAGA TTGAGACCAA CCCTGCAACC
    AGCTCAGTGT TTGGCAAGAC ACACAAGCTG AAGCCAGGAG TGAAGGAGCC TGTTGGTGTT
    AATAAAGCAG CACCTGCTCC ACCCGTTGAG GATGTTAGCA TGAGGATCTA TCTGTGTGAG
    GGGCTTCTGG GTAAAGAGCG CTCAACTCTG TGGGATCAGA TGCAGTTCTG GGAGGACGCC
    TTCCTGGATG CCGTAATGCT GGAACGGGAA GGAATGGGAA TGGATCAGGG ACCACAAGAA
    ATGATTGACA GGTATCTTTC TCTGGGAGAT CATGACCGCA AGAGACTGGA GGATGATGAG
    GACAGACTGC TGGCCACGCT TCTGCACAAT ATGATTGCTT ACATGCTTAT GATGAAGGTC
    GGTAAAAACG ACATCAGGAA GAAAGTACGT CGTCTTATGG GCAAATCCCA CATTGGCCTC
    ACTCACAGTC AGGAGATCAA TGAAGTCCTG GATCGCCTCG CACATTTAAG CGGTCGGGAG
    CTTTCTATCA GGCCGAGTGG CAGCAGGCAT ATTAAAAAAC AGACATTTGT TGTGCATGCT
    GGGACAGATA CAACTGGTGA CATATTCTTC ATGGAGGTGT GTGATGATTG CATCGTCTTG
    CGCAGTAATA TTGGCACAGT CTATGAGCGC TGGTGGTATG AGAAGCTCAT AAATATGACT
    TACTGTCCCA AAACTAAAGT GTTGTGTCTG TGGAGGAGAA ACGGCCAGGA GACGCAGCTA
    AACAAGTTTT ACACTAAGAA GTGTCGGGAA TTGTACTACT GTGTTAAAGA CAGCATGGAG
    CGTGCTGCTG CTCGACAACA AAGCATCAAA CCAGGTCCAG AGCTTGGAGG CGAGTTTCCG
    GTTCAGGACA TGAAGACAGG GGAGGGTGGT CTCCTCCAAG TCACACTGGA AGGCATCAAC
    CTCAAATTTA TGCACAGCCA GGTTTTCATA GAGCTGAATC ACATTAAAAA GTGCAATACA
    GTGAAAGGAG TCTTTGTCCT GGAGGAGTTT GTTCCTGAAA CTAAAGAAGT GGTGATCCAT
    AAGTACAAGA CTCCTATGGC ACATCAGATC TGTTACTCGG TGCTCTGCCT CTTCTCCTAC
    ATGGCGGCGG TCAAGGGGAA AGAGTCAGAG GGCAAACCCA AGATGCTGTC CCCTCGTCCA
    CTAGCGAGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327652.1
    CDS169..5040
    Translation

    Target ORF information:

    RefSeq Version XM_021471977.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X64, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471977.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA CTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGGACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCGGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT TCCCAAGCTC TGCTCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTCCCCAGC CTCAAAGGTG CCCGAGCCCC CAGAGCACTG
    GTAGATCAGA AATCCTCTGT TATTAAGCAC AGCCCTACTG TGAAGAGAGA GTCTCCGTCT
    CCTCAAGGCA GAGCCAACAA TACCAGTGAG AACCAGCAGT TTCTAAAGGA GGTGGTCCAG
    AGCGTGCTGG AGGGGCAGGG TGTGGGCTGG CTTAACATGA AGAAGGTGCG TCGCCTGCTA
    GAGAATGAAC AGCTCAGAGT CTTTGTGCTC AGCAAACTCA ACCGTGCGGT CCAGTCAGAG
    GAGGACGCCC AGCAGGAAGT TATAAGAGAT GTGGAGATAA ATAGAAAGGT CTACAAAGGC
    ATGCTGGATA TTTTGAAGTG CACGGTGTCC AGTTTGGAGC ATTCATACAC TAATGCTGGC
    TTAGGGGGCA TGGCCAGTGT CTTCAGTCTG CTAGAGATAG CACGCACCCA CTACCAAACC
    AAAGATCCAG AGAAGCGGAA ACGGAGCCCC ACCGAAGGGC TTAGCAGTCC AGGCAGTAAG
    GAGAGCCCAT CGGGCCGAAT GGAGAGTGCC AGGGCGGCAG GGGTGCTTCT GGTACCCCGT
    ATCCAATTGC CGCCACCCTC CTCTGGGAAA TCATCACGCC AGTTTGATAC CCGGAGTCTG
    AACGAGGAGA ATTTTATTGC CTCCATTGGA GCGGATGGAG TAAAACAACG TGTAGAAGGA
    GGAGACACTG AGGAGAAGAA ATCACAGATC AGTGCAGACA GTGGCCTCAG TGTGACATCT
    GGCTCACAGA AGAGTGATAC GGATTCCCTG GCGAGCTCTG AACCTCCTGC TCTGACCAGA
    AGCACCAGCC AGGACTCAGA GGCCAGCACA GTGGTAAGTA ACAGCTCAGG TGAGACGCTC
    GGTGCAGACA GTGACCTGAG CAGCACTGCT GGAGATGGCC TGACAGGAAG ACATGGTCAG
    CATCTCAACC TGTCCCGTGG CACACTGTCT GACAGTGAGA TTGAGACCAA CCCTGCAACC
    AGCTCAGTGT TTGGCAAGAC ACACAAGCTG AAGCCAGGAG TGAAGGAGCC TGTTGGTGTT
    AATAAAGCAG CACCTGCTCC ACCCGTTGAG GATGTTAGCA TGAGGATCTA TCTGTGTGAG
    GGGCTTCTGG GTAAAGAGCG CTCAACTCTG TGGGATCAGA TGCAGTTCTG GGAGGACGCC
    TTCCTGGATG CCGTAATGCT GGAACGGGAA GGAATGGGAA TGGATCAGGG ACCACAAGAA
    ATGATTGACA GGTATCTTTC TCTGGGAGAT CATGACCGCA AGAGACTGGA GGATGATGAG
    GACAGACTGC TGGCCACGCT TCTGCACAAT ATGATTGCTT ACATGCTTAT GATGAAGGTC
    GGTAAAAACG ACATCAGGAA GAAAGTACGT CGTCTTATGG GCAAATCCCA CATTGGCCTC
    ACTCACAGTC AGGAGATCAA TGAAGTCCTG GATCGCCTCG CACATTTAAG CGGTCGGGAG
    CTTTCTATCA GGCCGAGTGG CAGCAGGCAT ATTAAAAAAC AGACATTTGT TGTGCATGCT
    GGGACAGATA CAACTGGTGA CATATTCTTC ATGGAGGTGT GTGATGATTG CATCGTCTTG
    CGCAGTAATA TTGGCACAGT CTATGAGCGC TGGTGGTATG AGAAGCTCAT AAATATGACT
    TACTGTCCCA AAACTAAAGT GTTGTGTCTG TGGAGGAGAA ACGGCCAGGA GACGCAGCTA
    AACAAGTTTT ACACTAAGAA GTGTCGGGAA TTGTACTACT GTGTTAAAGA CAGCATGGAG
    CGTGCTGCTG CTCGACAACA AAGCATCAAA CCAGGTCCAG AGCTTGGAGG CGAGTTTCCG
    GTTCAGGACA TGAAGACAGG GGAGGGTGGT CTCCTCCAAG TCACACTGGA AGGCATCAAC
    CTCAAATTTA TGCACAGCCA GGTTTTCATA GAGCTGAATC ACATTAAAAA GTGCAATACA
    GTGAAAGGAG TCTTTGTCCT GGAGGAGTTT GTTCCTGAAA CTAAAGAAGT GGTGATCCAT
    AAGTACAAGA CTCCTATGGC ACATCAGATC TGTTACTCGG TGCTCTGCCT CTTCTCCTAC
    ATGGCGGCGG TCAAGGGGAA AGAGTCAGAG GGCAAACCCA AGATGCTGTC CCCTCGTCCA
    CTAGCGAGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144907
    Clone ID Related Accession (Same CDS sequence) XM_021471978.1
    Accession Version XM_021471978.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4869bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X26
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394698.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA CTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGGACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCGGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT TCCCAAGCTC TGCTCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTCCCCAGC CTCAAAGAAT ATTTTAATTT TGATTTAGCA
    GAGGACACAG ATCAAGACGA TGCTGATGAC AGTCCTGAAA GTCCTGTATT TGGGCTAAAT
    TCTCTAATGG AGATTGTAAC AGAGATCGGC CCGGGGAGCG GAGAAGGTGC CCGAGCCCCC
    AGAGCACTGG TAGATCAGAA ATCCTCTGTT ATTAAGCACA GCCCTACTGT GAAGAGAGAG
    TCTCCGTCTC CTCAAGGCAG AGCCAACAAT ACCAGTGAGA ACCAGCAGTT TCTAAAGGAG
    GTGGTCCAGA GCGTGCTGGA GGGGCAGGGT GTGGGCTGGC TTAACATGAA GAAGGTGCGT
    CGCCTGCTAG AGAATGAACA GCTCAGAGTC TTTGTGCTCA GCAAACTCAA CCGTGCGGTC
    CAGTCAGAGG AGGACGCCCA GCAGGAAGTT ATAAGAGATG TGGAGATAAA TAGAAAGGTC
    TACAAAGGCA TGCTGGATAT TTTGAAGTGC ACGGTGTCCA GTTTGGAGCA TTCATACACT
    AATGCTGGCT TAGGGGGCAT GGCCAGTGTC TTCAGTCTGC TAGAGATAGC ACGCACCCAC
    TACCAAACCA AAGGAGCGGA TGGAGTAAAA CAACGTGTAG AAGGAGGAGA CACTGAGGAG
    AAGAAATCAC AGATCAGTGC AGACAGTGGC CTCAGTGTGA CATCTGGCTC ACAGAAGAGT
    GATACGGATT CCCTGGCGAG CTCTGAACCT CCTGCTCTGA CCAGAAGCAC CAGCCAGGAC
    TCAGAGGCCA GCACAGTGGT AAGTAACAGC TCAGGTGAGA CGCTCGGTGC AGACAGTGAC
    CTGAGCAGCA CTGCTGGAGA TGGCCTGACA GGAAGACATG GTCAGCATCT CAACCTGTCC
    CGTGGCACAC TGTCTGACAG TGAGATTGAG ACCAACCCTG CAACCAGCTC AGTGTTTGGC
    AAGACACACA AGCTGAAGCC AGGAGTGAAG GAGCCTGTTG GTGTTAATAA AGCAGCACCT
    GCTCCACCCG TTGAGGATGT TAGCATGAGG ATCTATCTGT GTGAGGGGCT TCTGGGCCGG
    GATAAAAGCT CAGTTTGGGA CCAGTTGGAG GATGCTGCAA TGGAAACCTT TTCTCTGAGT
    AAAGAGCGCT CAACTCTGTG GGATCAGATG CAGTTCTGGG AGGACGCCTT CCTGGATGCC
    GTAATGCTGG AACGGGAAGG AATGGGAATG GATCAGGGAC CACAAGAAAT GATTGACAGG
    TATCTTTCTC TGGGAGATCA TGACCGCAAG AGACTGGAGG ATGATGAGGA CAGACTGCTG
    GCCACGCTTC TGCACAATAT GATTGCTTAC ATGCTTATGA TGAAGGTCGG TAAAAACGAC
    ATCAGGAAGA AAGTACGTCG TCTTATGGGC AAATCCCACA TTGGCCTCAC TCACAGTCAG
    GAGATCAATG AAGTCCTGGA TCGCCTCGCA CATTTAAGCG GTCGGGAGCT TTCTATCAGG
    CCGAGTGGCA GCAGGCATAT TAAAAAACAG ACATTTGTTG TGCATGCTGG GACAGATACA
    ACTGGTGACA TATTCTTCAT GGAGGTGTGT GATGATTGCA TCGTCTTGCG CAGTAATATT
    GGCACAGTCT ATGAGCGCTG GTGGTATGAG AAGCTCATAA ATATGACTTA CTGTCCCAAA
    ACTAAAGTGT TGTGTCTGTG GAGGAGAAAC GGCCAGGAGA CGCAGCTAAA CAAGTTTTAC
    ACTAAGAAGT GTCGGGAATT GTACTACTGT GTTAAAGACA GCATGGAGCG TGCTGCTGCT
    CGACAACAAA GCATCAAACC AGGTCCAGAG CTTGGAGGCG AGTTTCCGGT TCAGGACATG
    AAGACAGGGG AGGGTGGTCT CCTCCAAGTC ACACTGGAAG GCATCAACCT CAAATTTATG
    CACAGCCAGG AGCGGAAGGT TTTCATAGAG CTGAATCACA TTAAAAAGTG CAATACAGTG
    AAAGGAGTCT TTGTCCTGGA GGAGTTTGTT CCTGAAACTA AAGAAGTGGT GATCCATAAG
    TACAAGACTC CTATGGCACA TCAGATCTGT TACTCGGTGC TCTGCCTCTT CTCCTACATG
    GCGGCGGTCA AGGGGAAAGA GTCAGAGGGC AAACCCAAGA TGCTGTCCCC TCGTCCACTA
    GCGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327653.1
    CDS169..5037
    Translation

    Target ORF information:

    RefSeq Version XM_021471978.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X65, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471978.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA CTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGGACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCGGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT TCCCAAGCTC TGCTCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTCCCCAGC CTCAAAGAAT ATTTTAATTT TGATTTAGCA
    GAGGACACAG ATCAAGACGA TGCTGATGAC AGTCCTGAAA GTCCTGTATT TGGGCTAAAT
    TCTCTAATGG AGATTGTAAC AGAGATCGGC CCGGGGAGCG GAGAAGGTGC CCGAGCCCCC
    AGAGCACTGG TAGATCAGAA ATCCTCTGTT ATTAAGCACA GCCCTACTGT GAAGAGAGAG
    TCTCCGTCTC CTCAAGGCAG AGCCAACAAT ACCAGTGAGA ACCAGCAGTT TCTAAAGGAG
    GTGGTCCAGA GCGTGCTGGA GGGGCAGGGT GTGGGCTGGC TTAACATGAA GAAGGTGCGT
    CGCCTGCTAG AGAATGAACA GCTCAGAGTC TTTGTGCTCA GCAAACTCAA CCGTGCGGTC
    CAGTCAGAGG AGGACGCCCA GCAGGAAGTT ATAAGAGATG TGGAGATAAA TAGAAAGGTC
    TACAAAGGCA TGCTGGATAT TTTGAAGTGC ACGGTGTCCA GTTTGGAGCA TTCATACACT
    AATGCTGGCT TAGGGGGCAT GGCCAGTGTC TTCAGTCTGC TAGAGATAGC ACGCACCCAC
    TACCAAACCA AAGGAGCGGA TGGAGTAAAA CAACGTGTAG AAGGAGGAGA CACTGAGGAG
    AAGAAATCAC AGATCAGTGC AGACAGTGGC CTCAGTGTGA CATCTGGCTC ACAGAAGAGT
    GATACGGATT CCCTGGCGAG CTCTGAACCT CCTGCTCTGA CCAGAAGCAC CAGCCAGGAC
    TCAGAGGCCA GCACAGTGGT AAGTAACAGC TCAGGTGAGA CGCTCGGTGC AGACAGTGAC
    CTGAGCAGCA CTGCTGGAGA TGGCCTGACA GGAAGACATG GTCAGCATCT CAACCTGTCC
    CGTGGCACAC TGTCTGACAG TGAGATTGAG ACCAACCCTG CAACCAGCTC AGTGTTTGGC
    AAGACACACA AGCTGAAGCC AGGAGTGAAG GAGCCTGTTG GTGTTAATAA AGCAGCACCT
    GCTCCACCCG TTGAGGATGT TAGCATGAGG ATCTATCTGT GTGAGGGGCT TCTGGGCCGG
    GATAAAAGCT CAGTTTGGGA CCAGTTGGAG GATGCTGCAA TGGAAACCTT TTCTCTGAGT
    AAAGAGCGCT CAACTCTGTG GGATCAGATG CAGTTCTGGG AGGACGCCTT CCTGGATGCC
    GTAATGCTGG AACGGGAAGG AATGGGAATG GATCAGGGAC CACAAGAAAT GATTGACAGG
    TATCTTTCTC TGGGAGATCA TGACCGCAAG AGACTGGAGG ATGATGAGGA CAGACTGCTG
    GCCACGCTTC TGCACAATAT GATTGCTTAC ATGCTTATGA TGAAGGTCGG TAAAAACGAC
    ATCAGGAAGA AAGTACGTCG TCTTATGGGC AAATCCCACA TTGGCCTCAC TCACAGTCAG
    GAGATCAATG AAGTCCTGGA TCGCCTCGCA CATTTAAGCG GTCGGGAGCT TTCTATCAGG
    CCGAGTGGCA GCAGGCATAT TAAAAAACAG ACATTTGTTG TGCATGCTGG GACAGATACA
    ACTGGTGACA TATTCTTCAT GGAGGTGTGT GATGATTGCA TCGTCTTGCG CAGTAATATT
    GGCACAGTCT ATGAGCGCTG GTGGTATGAG AAGCTCATAA ATATGACTTA CTGTCCCAAA
    ACTAAAGTGT TGTGTCTGTG GAGGAGAAAC GGCCAGGAGA CGCAGCTAAA CAAGTTTTAC
    ACTAAGAAGT GTCGGGAATT GTACTACTGT GTTAAAGACA GCATGGAGCG TGCTGCTGCT
    CGACAACAAA GCATCAAACC AGGTCCAGAG CTTGGAGGCG AGTTTCCGGT TCAGGACATG
    AAGACAGGGG AGGGTGGTCT CCTCCAAGTC ACACTGGAAG GCATCAACCT CAAATTTATG
    CACAGCCAGG AGCGGAAGGT TTTCATAGAG CTGAATCACA TTAAAAAGTG CAATACAGTG
    AAAGGAGTCT TTGTCCTGGA GGAGTTTGTT CCTGAAACTA AAGAAGTGGT GATCCATAAG
    TACAAGACTC CTATGGCACA TCAGATCTGT TACTCGGTGC TCTGCCTCTT CTCCTACATG
    GCGGCGGTCA AGGGGAAAGA GTCAGAGGGC AAACCCAAGA TGCTGTCCCC TCGTCCACTA
    GCGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144908
    Clone ID Related Accession (Same CDS sequence) XM_021471979.1
    Accession Version XM_021471979.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4869bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X27
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394698.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA CTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGGACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCGGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT TCCCAAGCTC TGCTCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTCCCCAGC CTCAAAGGTG CCCGAGCCCC CAGAGCACTG
    GTAGATCAGA AATCCTCTGT TATTAAGCAC AGCCCTACTG TGAAGAGAGA GTCTCCGTCT
    CCTCAAGGCA GAGCCAACAA TACCAGTGAG AACCAGCAGT TTCTAAAGGA GGTGGTCCAG
    AGCGTGCTGG AGGGGCAGGG TGTGGGCTGG CTTAACATGA AGAAGGTGCG TCGCCTGCTA
    GAGAATGAAC AGCTCAGAGT CTTTGTGCTC AGCAAACTCA ACCGTGCGGT CCAGTCAGAG
    GAGGACGCCC AGCAGGAAGT TATAAGAGAT GTGGAGATAA ATAGAAAGGT CTACAAAGGC
    ATGCTGGATA TTTTGAAGTG CACGGTGTCC AGTTTGGAGC ATTCATACAC TAATGCTGGC
    TTAGGGGGCA TGGCCAGTGT CTTCAGTCTG CTAGAGATAG CACGCACCCA CTACCAAACC
    AAAGATCCAG AGAAGCGGAA ACGGAGCCCC ACCGAAGGGC TTAGCAGTCC AGGCAGTAAG
    GAGAGCCCAT CGGGCCGAAT GGAGAGTGCC AGGGCGGCAG GGGTGCTTCT GGTACCCCGT
    ATCCAATTGC CGCCACCCTC CTCTGGGAAA TCATCACGCC AGTTTGATAC CCGGAGTCTG
    AACGAGGAGA ATTTTATTGC CTCCATTGGA GCGGATGGAG TAAAACAACG TGTAGAAGGA
    GGAGACACTG AGGAGAAGAA ATCACAGATC AGTGCAGACA GTGGCCTCAG TGTGACATCT
    GGCTCACAGA AGAGTGATAC GGATTCCCTG GCGAGCTCTG AACCTCCTGC TCTGACCAGA
    AGCACCAGCC AGGACTCAGA GGCCAGCACA GTAAGTAACA GCTCAGGTGA GACGCTCGGT
    GCAGACAGTG ACCTGAGCAG CACTGCTGGA GATGGCCTGA CAGGAAGACA TGGTCAGCAT
    CTCAACCTGT CCCGTGGCAC ACTGTCTGAC AGTGAGATTG AGACCAACCC TGCAACCAGC
    TCAGTGTTTG GCAAGACACA CAAGCTGAAG CCAGGAGTGA AGGAGCCTGT TGGTGTTAAT
    AAAGCAGCAC CTGCTCCACC CGTTGAGGAT GTTAGCATGA GGATCTATCT GTGTGAGGGG
    CTTCTGGGTA AAGAGCGCTC AACTCTGTGG GATCAGATGC AGTTCTGGGA GGACGCCTTC
    CTGGATGCCG TAATGCTGGA ACGGGAAGGA ATGGGAATGG ATCAGGGACC ACAAGAAATG
    ATTGACAGGT ATCTTTCTCT GGGAGATCAT GACCGCAAGA GACTGGAGGA TGATGAGGAC
    AGACTGCTGG CCACGCTTCT GCACAATATG ATTGCTTACA TGCTTATGAT GAAGGTCGGT
    AAAAACGACA TCAGGAAGAA AGTACGTCGT CTTATGGGCA AATCCCACAT TGGCCTCACT
    CACAGTCAGG AGATCAATGA AGTCCTGGAT CGCCTCGCAC ATTTAAGCGG TCGGGAGCTT
    TCTATCAGGC CGAGTGGCAG CAGGCATATT AAAAAACAGA CATTTGTTGT GCATGCTGGG
    ACAGATACAA CTGGTGACAT ATTCTTCATG GAGGTGTGTG ATGATTGCAT CGTCTTGCGC
    AGTAATATTG GCACAGTCTA TGAGCGCTGG TGGTATGAGA AGCTCATAAA TATGACTTAC
    TGTCCCAAAA CTAAAGTGTT GTGTCTGTGG AGGAGAAACG GCCAGGAGAC GCAGCTAAAC
    AAGTTTTACA CTAAGAAGTG TCGGGAATTG TACTACTGTG TTAAAGACAG CATGGAGCGT
    GCTGCTGCTC GACAACAAAG CATCAAACCA GGTCCAGAGC TTGGAGGCGA GTTTCCGGTT
    CAGGACATGA AGACAGGGGA GGGTGGTCTC CTCCAAGTCA CACTGGAAGG CATCAACCTC
    AAATTTATGC ACAGCCAGGT TTTCATAGAG CTGAATCACA TTAAAAAGTG CAATACAGTG
    AAAGGAGTCT TTGTCCTGGA GGAGTTTGTT CCTGAAACTA AAGAAGTGGT GATCCATAAG
    TACAAGACTC CTATGGCACA TCAGATCTGT TACTCGGTGC TCTGCCTCTT CTCCTACATG
    GCGGCGGTCA AGGGGAAAGA GTCAGAGGGC AAACCCAAGA TGCTGTCCCC TCGTCCACTA
    GCGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327654.1
    CDS169..5037
    Translation

    Target ORF information:

    RefSeq Version XM_021471979.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X66, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471979.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGTGT
    CTGGTAAGGT TGACTCTGAT CACCCAAAAG CAGATCTTCG CCTCCGATTC TAAGGCTCTG
    GCTAGCATGA GTCTAAACAC ACAGCCTATT CTGAACCTGG AGAAGTTTCA GGATGGACAG
    GAGTTGTCTT TATTGCCCCC CGGTCGTGAC AAAGCTTCCC CGTCCTCCAC TGAGTTCAAC
    CCACTCATCT ACGGCAATGA TGTGGACTCA GTTGATGTTG CCACAAGGGT GGCAATGGTG
    AGGTTCTTCA ACTCTCCAAA CGTGCTGCAA GGGTTCCAGA TGCACACACG CACTCTCCGT
    CTCTTCCCTC GTCCTGTGGT GGCCTTTCAG TCCACATCTT TCCTGGCTTC TAGGCCACGA
    CGAAATGGAT TCACAGAAAA GCTCTCCCAC ACTCAGGCAG TGGAGTACTA TGGAGAATGG
    GCTCTCAACC CCACCAACCT GGCTTTCCAA AGGATTCATA ACAATGTGTA TGACCCCTCT
    CTAATAGGCG ATAAGCCTAA GTGGTATGCC CACCAGTTAC AGCCAGTATT CTACCGCGTG
    TATGATGGAA ACTCTCATTT AGCGGAGGCC TTGAGTGGAC CCCTGCAGGA TGAGACCAAT
    GATTCAGACC CAACAGACGA CAGTGGCAGC GACAGTGAAG CATATGATGA CTCCAGCTCC
    TCCTACTCAT CTCTTGGAGA CTTTGTGAAT GAGATGATCA AAGGGGACAT ACAGGGAGAC
    ACACCAAATG TTGATCCCCT GACCCATGCT GCACTGGGAG ATGCAGATGA GGTTGAGATC
    CATGACTTTC ACGAGTATAA GGGGGACAGC GGTGAACCAG AGCCAGAAGG CCCCACAGAA
    GCGGCGGACA GTCAGCCCTT GCGGTCCAGC TCTAGCACCA CAGCCAGTTC CAGCCCCAGT
    ACAGTCATAC AGGGAGTCAA CCATGAGCAG AAGGAACCTG TTGAGGTGGA GCCCATAGCA
    AATGTGACTT TCCCAAGCTC TGCTCCAGTA TTGGGTCCTC CTCCTTTTAC AAGACCCACC
    CCTGATCCAA CCCTTGTTGA CCCTGCCAAT AAGAAGCGAG AATATGACAA CCCCTATTTT
    GAGCCCCAGT ACGGTTTCCC TACAGAGGAA GATGCTGAGA CTGATGAGCA GGAGGAGAGT
    TACACACCAC GATTTAACCA GAATCTCAAT GGCAATAAGC CTTCTCGTCC ACTGAGACCC
    AGCAGCCTGA AGCTTCCGGG TGAATCTGAC GGAGAGGGAG ACTCCAGAAA TAGCTCTCCT
    AACTCAACCA TCTCCAACAA TAGCAGTGAT GGCTTTGGGG GACTCATGTC CTTTGCCAGT
    AACTTGTATA AAAACCATGG CACCAGTTTC AGTCTGTCCA GCCTGGCTCT GCCTAATAAA
    GCGAGAGAGA AGAACACGCC GTTCCCCAGC CTCAAAGGTG CCCGAGCCCC CAGAGCACTG
    GTAGATCAGA AATCCTCTGT TATTAAGCAC AGCCCTACTG TGAAGAGAGA GTCTCCGTCT
    CCTCAAGGCA GAGCCAACAA TACCAGTGAG AACCAGCAGT TTCTAAAGGA GGTGGTCCAG
    AGCGTGCTGG AGGGGCAGGG TGTGGGCTGG CTTAACATGA AGAAGGTGCG TCGCCTGCTA
    GAGAATGAAC AGCTCAGAGT CTTTGTGCTC AGCAAACTCA ACCGTGCGGT CCAGTCAGAG
    GAGGACGCCC AGCAGGAAGT TATAAGAGAT GTGGAGATAA ATAGAAAGGT CTACAAAGGC
    ATGCTGGATA TTTTGAAGTG CACGGTGTCC AGTTTGGAGC ATTCATACAC TAATGCTGGC
    TTAGGGGGCA TGGCCAGTGT CTTCAGTCTG CTAGAGATAG CACGCACCCA CTACCAAACC
    AAAGATCCAG AGAAGCGGAA ACGGAGCCCC ACCGAAGGGC TTAGCAGTCC AGGCAGTAAG
    GAGAGCCCAT CGGGCCGAAT GGAGAGTGCC AGGGCGGCAG GGGTGCTTCT GGTACCCCGT
    ATCCAATTGC CGCCACCCTC CTCTGGGAAA TCATCACGCC AGTTTGATAC CCGGAGTCTG
    AACGAGGAGA ATTTTATTGC CTCCATTGGA GCGGATGGAG TAAAACAACG TGTAGAAGGA
    GGAGACACTG AGGAGAAGAA ATCACAGATC AGTGCAGACA GTGGCCTCAG TGTGACATCT
    GGCTCACAGA AGAGTGATAC GGATTCCCTG GCGAGCTCTG AACCTCCTGC TCTGACCAGA
    AGCACCAGCC AGGACTCAGA GGCCAGCACA GTAAGTAACA GCTCAGGTGA GACGCTCGGT
    GCAGACAGTG ACCTGAGCAG CACTGCTGGA GATGGCCTGA CAGGAAGACA TGGTCAGCAT
    CTCAACCTGT CCCGTGGCAC ACTGTCTGAC AGTGAGATTG AGACCAACCC TGCAACCAGC
    TCAGTGTTTG GCAAGACACA CAAGCTGAAG CCAGGAGTGA AGGAGCCTGT TGGTGTTAAT
    AAAGCAGCAC CTGCTCCACC CGTTGAGGAT GTTAGCATGA GGATCTATCT GTGTGAGGGG
    CTTCTGGGTA AAGAGCGCTC AACTCTGTGG GATCAGATGC AGTTCTGGGA GGACGCCTTC
    CTGGATGCCG TAATGCTGGA ACGGGAAGGA ATGGGAATGG ATCAGGGACC ACAAGAAATG
    ATTGACAGGT ATCTTTCTCT GGGAGATCAT GACCGCAAGA GACTGGAGGA TGATGAGGAC
    AGACTGCTGG CCACGCTTCT GCACAATATG ATTGCTTACA TGCTTATGAT GAAGGTCGGT
    AAAAACGACA TCAGGAAGAA AGTACGTCGT CTTATGGGCA AATCCCACAT TGGCCTCACT
    CACAGTCAGG AGATCAATGA AGTCCTGGAT CGCCTCGCAC ATTTAAGCGG TCGGGAGCTT
    TCTATCAGGC CGAGTGGCAG CAGGCATATT AAAAAACAGA CATTTGTTGT GCATGCTGGG
    ACAGATACAA CTGGTGACAT ATTCTTCATG GAGGTGTGTG ATGATTGCAT CGTCTTGCGC
    AGTAATATTG GCACAGTCTA TGAGCGCTGG TGGTATGAGA AGCTCATAAA TATGACTTAC
    TGTCCCAAAA CTAAAGTGTT GTGTCTGTGG AGGAGAAACG GCCAGGAGAC GCAGCTAAAC
    AAGTTTTACA CTAAGAAGTG TCGGGAATTG TACTACTGTG TTAAAGACAG CATGGAGCGT
    GCTGCTGCTC GACAACAAAG CATCAAACCA GGTCCAGAGC TTGGAGGCGA GTTTCCGGTT
    CAGGACATGA AGACAGGGGA GGGTGGTCTC CTCCAAGTCA CACTGGAAGG CATCAACCTC
    AAATTTATGC ACAGCCAGGT TTTCATAGAG CTGAATCACA TTAAAAAGTG CAATACAGTG
    AAAGGAGTCT TTGTCCTGGA GGAGTTTGTT CCTGAAACTA AAGAAGTGGT GATCCATAAG
    TACAAGACTC CTATGGCACA TCAGATCTGT TACTCGGTGC TCTGCCTCTT CTCCTACATG
    GCGGCGGTCA AGGGGAAAGA GTCAGAGGGC AAACCCAAGA TGCTGTCCCC TCGTCCACTA
    GCGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144911
    Clone ID Related Accession (Same CDS sequence) XM_021471982.1
    Accession Version XM_021471982.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4806bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X30
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394698.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGACTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGGA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCGGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTTCCCAA GCTCTGCTCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTCCC CAGCCTCAAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGGAGCGGAT GGAGTAAAAC AACGTGTAGA AGGAGGAGAC
    ACTGAGGAGA AGAAATCACA GATCAGTGCA GACAGTGGCC TCAGTGTGAC ATCTGGCTCA
    CAGAAGAGTG ATACGGATTC CCTGGCGAGC TCTGAACCTC CTGCTCTGAC CAGAAGCACC
    AGCCAGGACT CAGAGGCCAG CACAGTGGTA AGTAACAGCT CAGGTGAGAC GCTCGGTGCA
    GACAGTGACC TGAGCAGCAC TGCTGGAGAT GGCCTGACAG GAAGACATGG TCAGCATCTC
    AACCTGTCCC GTGGCACACT GTCTGACAGT GAGATTGAGA CCAACCCTGC AACCAGCTCA
    GTGTTTGGCA AGACACACAA GCTGAAGCCA GGAGTGAAGG AGCCTGTTGG TGTTAATAAA
    GCAGCACCTG CTCCACCCGT TGAGGATGTT AGCATGAGGA TCTATCTGTG TGAGGGGCTT
    CTGGGTAAAG AGCGCTCAAC TCTGTGGGAT CAGATGCAGT TCTGGGAGGA CGCCTTCCTG
    GATGCCGTAA TGCTGGAACG GGAAGGAATG GGAATGGATC AGGGACCACA AGAAATGATT
    GACAGGTATC TTTCTCTGGG AGATCATGAC CGCAAGAGAC TGGAGGATGA TGAGGACAGA
    CTGCTGGCCA CGCTTCTGCA CAATATGATT GCTTACATGC TTATGATGAA GGTCGGTAAA
    AACGACATCA GGAAGAAAGT ACGTCGTCTT ATGGGCAAAT CCCACATTGG CCTCACTCAC
    AGTCAGGAGA TCAATGAAGT CCTGGATCGC CTCGCACATT TAAGCGGTCG GGAGCTTTCT
    ATCAGGCCGA GTGGCAGCAG GCATATTAAA AAACAGACAT TTGTTGTGCA TGCTGGGACA
    GATACAACTG GTGACATATT CTTCATGGAG GTGTGTGATG ATTGCATCGT CTTGCGCAGT
    AATATTGGCA CAGTCTATGA GCGCTGGTGG TATGAGAAGC TCATAAATAT GACTTACTGT
    CCCAAAACTA AAGTGTTGTG TCTGTGGAGG AGAAACGGCC AGGAGACGCA GCTAAACAAG
    TTTTACACTA AGAAGTGTCG GGAATTGTAC TACTGTGTTA AAGACAGCAT GGAGCGTGCT
    GCTGCTCGAC AACAAAGCAT CAAACCAGGT CCAGAGCTTG GAGGCGAGTT TCCGGTTCAG
    GACATGAAGA CAGGGGAGGG TGGTCTCCTC CAAGTCACAC TGGAAGGCAT CAACCTCAAA
    TTTATGCACA GCCAGGTTTT CATAGAGCTG AATCACATTA AAAAGTGCAA TACAGTGAAA
    GGAGTCTTTG TCCTGGAGGA GTTTGTTCCT GAAACTAAAG AAGTGGTGAT CCATAAGTAC
    AAGACTCCTA TGGCACATCA GATCTGTTAC TCGGTGCTCT GCCTCTTCTC CTACATGGCG
    GCGGTCAAGG GGAAAGAGTC AGAGGGCAAA CCCAAGATGC TGTCCCCTCG TCCACTAGCG
    AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327657.1
    CDS1..4806
    Translation

    Target ORF information:

    RefSeq Version XM_021471982.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471982.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGACTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGGA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCGGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTTCCCAA GCTCTGCTCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTCCC CAGCCTCAAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGGAGCGGAT GGAGTAAAAC AACGTGTAGA AGGAGGAGAC
    ACTGAGGAGA AGAAATCACA GATCAGTGCA GACAGTGGCC TCAGTGTGAC ATCTGGCTCA
    CAGAAGAGTG ATACGGATTC CCTGGCGAGC TCTGAACCTC CTGCTCTGAC CAGAAGCACC
    AGCCAGGACT CAGAGGCCAG CACAGTGGTA AGTAACAGCT CAGGTGAGAC GCTCGGTGCA
    GACAGTGACC TGAGCAGCAC TGCTGGAGAT GGCCTGACAG GAAGACATGG TCAGCATCTC
    AACCTGTCCC GTGGCACACT GTCTGACAGT GAGATTGAGA CCAACCCTGC AACCAGCTCA
    GTGTTTGGCA AGACACACAA GCTGAAGCCA GGAGTGAAGG AGCCTGTTGG TGTTAATAAA
    GCAGCACCTG CTCCACCCGT TGAGGATGTT AGCATGAGGA TCTATCTGTG TGAGGGGCTT
    CTGGGTAAAG AGCGCTCAAC TCTGTGGGAT CAGATGCAGT TCTGGGAGGA CGCCTTCCTG
    GATGCCGTAA TGCTGGAACG GGAAGGAATG GGAATGGATC AGGGACCACA AGAAATGATT
    GACAGGTATC TTTCTCTGGG AGATCATGAC CGCAAGAGAC TGGAGGATGA TGAGGACAGA
    CTGCTGGCCA CGCTTCTGCA CAATATGATT GCTTACATGC TTATGATGAA GGTCGGTAAA
    AACGACATCA GGAAGAAAGT ACGTCGTCTT ATGGGCAAAT CCCACATTGG CCTCACTCAC
    AGTCAGGAGA TCAATGAAGT CCTGGATCGC CTCGCACATT TAAGCGGTCG GGAGCTTTCT
    ATCAGGCCGA GTGGCAGCAG GCATATTAAA AAACAGACAT TTGTTGTGCA TGCTGGGACA
    GATACAACTG GTGACATATT CTTCATGGAG GTGTGTGATG ATTGCATCGT CTTGCGCAGT
    AATATTGGCA CAGTCTATGA GCGCTGGTGG TATGAGAAGC TCATAAATAT GACTTACTGT
    CCCAAAACTA AAGTGTTGTG TCTGTGGAGG AGAAACGGCC AGGAGACGCA GCTAAACAAG
    TTTTACACTA AGAAGTGTCG GGAATTGTAC TACTGTGTTA AAGACAGCAT GGAGCGTGCT
    GCTGCTCGAC AACAAAGCAT CAAACCAGGT CCAGAGCTTG GAGGCGAGTT TCCGGTTCAG
    GACATGAAGA CAGGGGAGGG TGGTCTCCTC CAAGTCACAC TGGAAGGCAT CAACCTCAAA
    TTTATGCACA GCCAGGTTTT CATAGAGCTG AATCACATTA AAAAGTGCAA TACAGTGAAA
    GGAGTCTTTG TCCTGGAGGA GTTTGTTCCT GAAACTAAAG AAGTGGTGAT CCATAAGTAC
    AAGACTCCTA TGGCACATCA GATCTGTTAC TCGGTGCTCT GCCTCTTCTC CTACATGGCG
    GCGGTCAAGG GGAAAGAGTC AGAGGGCAAA CCCAAGATGC TGTCCCCTCG TCCACTAGCG
    AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144912
    Clone ID Related Accession (Same CDS sequence) XM_021471984.1
    Accession Version XM_021471984.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4803bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X31
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394698.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGACTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGGA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCGGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTTCCCAA GCTCTGCTCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTCCC CAGCCTCAAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGGAGCGGAT GGAGTAAAAC AACGTGTAGA AGGAGGAGAC
    ACTGAGGAGA AGAAATCACA GATCAGTGCA GACAGTGGCC TCAGTGTGAC ATCTGGCTCA
    CAGAAGAGTG ATACGGATTC CCTGGCGAGC TCTGAACCTC CTGCTCTGAC CAGAAGCACC
    AGCCAGGACT CAGAGGCCAG CACAGTAAGT AACAGCTCAG GTGAGACGCT CGGTGCAGAC
    AGTGACCTGA GCAGCACTGC TGGAGATGGC CTGACAGGAA GACATGGTCA GCATCTCAAC
    CTGTCCCGTG GCACACTGTC TGACAGTGAG ATTGAGACCA ACCCTGCAAC CAGCTCAGTG
    TTTGGCAAGA CACACAAGCT GAAGCCAGGA GTGAAGGAGC CTGTTGGTGT TAATAAAGCA
    GCACCTGCTC CACCCGTTGA GGATGTTAGC ATGAGGATCT ATCTGTGTGA GGGGCTTCTG
    GGTAAAGAGC GCTCAACTCT GTGGGATCAG ATGCAGTTCT GGGAGGACGC CTTCCTGGAT
    GCCGTAATGC TGGAACGGGA AGGAATGGGA ATGGATCAGG GACCACAAGA AATGATTGAC
    AGGTATCTTT CTCTGGGAGA TCATGACCGC AAGAGACTGG AGGATGATGA GGACAGACTG
    CTGGCCACGC TTCTGCACAA TATGATTGCT TACATGCTTA TGATGAAGGT CGGTAAAAAC
    GACATCAGGA AGAAAGTACG TCGTCTTATG GGCAAATCCC ACATTGGCCT CACTCACAGT
    CAGGAGATCA ATGAAGTCCT GGATCGCCTC GCACATTTAA GCGGTCGGGA GCTTTCTATC
    AGGCCGAGTG GCAGCAGGCA TATTAAAAAA CAGACATTTG TTGTGCATGC TGGGACAGAT
    ACAACTGGTG ACATATTCTT CATGGAGGTG TGTGATGATT GCATCGTCTT GCGCAGTAAT
    ATTGGCACAG TCTATGAGCG CTGGTGGTAT GAGAAGCTCA TAAATATGAC TTACTGTCCC
    AAAACTAAAG TGTTGTGTCT GTGGAGGAGA AACGGCCAGG AGACGCAGCT AAACAAGTTT
    TACACTAAGA AGTGTCGGGA ATTGTACTAC TGTGTTAAAG ACAGCATGGA GCGTGCTGCT
    GCTCGACAAC AAAGCATCAA ACCAGGTCCA GAGCTTGGAG GCGAGTTTCC GGTTCAGGAC
    ATGAAGACAG GGGAGGGTGG TCTCCTCCAA GTCACACTGG AAGGCATCAA CCTCAAATTT
    ATGCACAGCC AGGTTTTCAT AGAGCTGAAT CACATTAAAA AGTGCAATAC AGTGAAAGGA
    GTCTTTGTCC TGGAGGAGTT TGTTCCTGAA ACTAAAGAAG TGGTGATCCA TAAGTACAAG
    ACTCCTATGG CACATCAGAT CTGTTACTCG GTGCTCTGCC TCTTCTCCTA CATGGCGGCG
    GTCAAGGGGA AAGAGTCAGA GGGCAAACCC AAGATGCTGT CCCCTCGTCC ACTAGCGAGC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327659.1
    CDS1..4803
    Translation

    Target ORF information:

    RefSeq Version XM_021471984.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471984.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGACTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGGA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCGGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTTCCCAA GCTCTGCTCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTCCC CAGCCTCAAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGGAGCGGAT GGAGTAAAAC AACGTGTAGA AGGAGGAGAC
    ACTGAGGAGA AGAAATCACA GATCAGTGCA GACAGTGGCC TCAGTGTGAC ATCTGGCTCA
    CAGAAGAGTG ATACGGATTC CCTGGCGAGC TCTGAACCTC CTGCTCTGAC CAGAAGCACC
    AGCCAGGACT CAGAGGCCAG CACAGTAAGT AACAGCTCAG GTGAGACGCT CGGTGCAGAC
    AGTGACCTGA GCAGCACTGC TGGAGATGGC CTGACAGGAA GACATGGTCA GCATCTCAAC
    CTGTCCCGTG GCACACTGTC TGACAGTGAG ATTGAGACCA ACCCTGCAAC CAGCTCAGTG
    TTTGGCAAGA CACACAAGCT GAAGCCAGGA GTGAAGGAGC CTGTTGGTGT TAATAAAGCA
    GCACCTGCTC CACCCGTTGA GGATGTTAGC ATGAGGATCT ATCTGTGTGA GGGGCTTCTG
    GGTAAAGAGC GCTCAACTCT GTGGGATCAG ATGCAGTTCT GGGAGGACGC CTTCCTGGAT
    GCCGTAATGC TGGAACGGGA AGGAATGGGA ATGGATCAGG GACCACAAGA AATGATTGAC
    AGGTATCTTT CTCTGGGAGA TCATGACCGC AAGAGACTGG AGGATGATGA GGACAGACTG
    CTGGCCACGC TTCTGCACAA TATGATTGCT TACATGCTTA TGATGAAGGT CGGTAAAAAC
    GACATCAGGA AGAAAGTACG TCGTCTTATG GGCAAATCCC ACATTGGCCT CACTCACAGT
    CAGGAGATCA ATGAAGTCCT GGATCGCCTC GCACATTTAA GCGGTCGGGA GCTTTCTATC
    AGGCCGAGTG GCAGCAGGCA TATTAAAAAA CAGACATTTG TTGTGCATGC TGGGACAGAT
    ACAACTGGTG ACATATTCTT CATGGAGGTG TGTGATGATT GCATCGTCTT GCGCAGTAAT
    ATTGGCACAG TCTATGAGCG CTGGTGGTAT GAGAAGCTCA TAAATATGAC TTACTGTCCC
    AAAACTAAAG TGTTGTGTCT GTGGAGGAGA AACGGCCAGG AGACGCAGCT AAACAAGTTT
    TACACTAAGA AGTGTCGGGA ATTGTACTAC TGTGTTAAAG ACAGCATGGA GCGTGCTGCT
    GCTCGACAAC AAAGCATCAA ACCAGGTCCA GAGCTTGGAG GCGAGTTTCC GGTTCAGGAC
    ATGAAGACAG GGGAGGGTGG TCTCCTCCAA GTCACACTGG AAGGCATCAA CCTCAAATTT
    ATGCACAGCC AGGTTTTCAT AGAGCTGAAT CACATTAAAA AGTGCAATAC AGTGAAAGGA
    GTCTTTGTCC TGGAGGAGTT TGTTCCTGAA ACTAAAGAAG TGGTGATCCA TAAGTACAAG
    ACTCCTATGG CACATCAGAT CTGTTACTCG GTGCTCTGCC TCTTCTCCTA CATGGCGGCG
    GTCAAGGGGA AAGAGTCAGA GGGCAAACCC AAGATGCTGT CCCCTCGTCC ACTAGCGAGC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144914
    Clone ID Related Accession (Same CDS sequence) XM_021471986.1
    Accession Version XM_021471986.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5010bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X33
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394698.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGACTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGGA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCGGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTTCCCAA GCTCTGCTCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTCCC CAGCCTCAAA GTATTTGGGC TAAATTCTCT AATGGAGATT
    GTAACAGAGA TCGGCCCGGG GAGCGGAGAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGAATTGTGG AGCAAGCACC AGGATAACAG AAAGCAAAAA
    GCTTTGGAAA AAGAACAGAG TGGTTTAAAT AAACGGAAAT CAGGAGCGGA TGGAGTAAAA
    CAACGTGTAG AAGGAGGAGA CACTGAGGAG AAGAAATCAC AGATCAGTGC AGACAGTGGC
    CTCAGTGTGA CATCTGGCTC ACAGAAGAGT GATACGGATT CCCTGGCGAG CTCTGAACCT
    CCTGCTCTGA CCAGAAGCAC CAGCCAGGAC TCAGAGGCCA GCACAGTGGT AAGTAACAGC
    TCAGGTGAGA CGCTCGGTGC AGACAGTGAC CTGAGCAGCA CTGCTGGAGA TGGCCTGACA
    GGAAGACATG GTCAGCATCT CAACCTGTCC CGTGGCACAC TGTCTGACAG TGAGATTGAG
    ACCAACCCTG CAACCAGCTC AGTGTTTGGC AAGACACACA AGCTGAAGCC AGGAGTGAAG
    GAGCCTGTTG GTGTTAATAA AGCAGCACCT GCTCCACCCG TTGAGGATGT TAGCATGAGG
    ATCTATCTGT GTGAGGGGCT TCTGGGCCGG GATAAAAGCT CAGTTTGGGA CCAGTTGGAG
    GATGCTGCAA TGGAAACCTT TTCTCTGAGT AAAGAGCGCT CAACTCTGTG GGATCAGATG
    CAGTTCTGGG AGGACGCCTT CCTGGATGCC GTAATGCTGG AACGGGAAGG AATGGGAATG
    GATCAGGGAC CACAAGAAAT GATTGACAGG TATCTTTCTC TGGGAGATCA TGACCGCAAG
    AGACTGGAGG ATGATGAGGA CAGACTGCTG GCCACGCTTC TGCACAATAT GATTGCTTAC
    ATGCTTATGA TGAAGGTCGG TAAAAACGAC ATCAGGAAGA AAGTACGTCG TCTTATGGGC
    AAATCCCACA TTGGCCTCAC TCACAGTCAG GAGATCAATG AAGTCCTGGA TCGCCTCGCA
    CATTTAAGCG GTCGGGAGCT TTCTATCAGG CCGAGTGGCA GCAGGCATAT TAAAAAACAG
    ACATTTGTTG TGCATGCTGG GACAGATACA ACTGGTGACA TATTCTTCAT GGAGGTGTGT
    GATGATTGCA TCGTCTTGCG CAGTAATATT GGCACAGTCT ATGAGCGCTG GTGGTATGAG
    AAGCTCATAA ATATGACTTA CTGTCCCAAA ACTAAAGTGT TGTGTCTGTG GAGGAGAAAC
    GGCCAGGAGA CGCAGCTAAA CAAGTTTTAC ACTAAGAAGT GTCGGGAATT GTACTACTGT
    GTTAAAGACA GCATGGAGCG TGCTGCTGCT CGACAACAAA GCATCAAACC AGGTCCAGAG
    CTTGGAGGCG AGTTTCCGGT TCAGGACATG AAGACAGGGG AGGGTGGTCT CCTCCAAGTC
    ACACTGGAAG GCATCAACCT CAAATTTATG CACAGCCAGG TTTTCATAGA GCTGAATCAC
    ATTAAAAAGT GCAATACAGT GAAAGGAGTC TTTGTCCTGG AGGAGTTTGT TCCTGAAACT
    AAAGAAGTGG TGATCCATAA GTACAAGACT CCTATGGCAC ATCAGATCTG TTACTCGGTG
    CTCTGCCTCT TCTCCTACAT GGCGGCGGTC AAGGGGAAAG AGTCAGAGGG CAAACCCAAG
    ATGCTGTCCC CTCGTCCACT AGCGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327661.1
    CDS169..5178
    Translation

    Target ORF information:

    RefSeq Version XM_021471986.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X68, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471986.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGACTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGGA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCGGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTTCCCAA GCTCTGCTCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTCCC CAGCCTCAAA GTATTTGGGC TAAATTCTCT AATGGAGATT
    GTAACAGAGA TCGGCCCGGG GAGCGGAGAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGAATTGTGG AGCAAGCACC AGGATAACAG AAAGCAAAAA
    GCTTTGGAAA AAGAACAGAG TGGTTTAAAT AAACGGAAAT CAGGAGCGGA TGGAGTAAAA
    CAACGTGTAG AAGGAGGAGA CACTGAGGAG AAGAAATCAC AGATCAGTGC AGACAGTGGC
    CTCAGTGTGA CATCTGGCTC ACAGAAGAGT GATACGGATT CCCTGGCGAG CTCTGAACCT
    CCTGCTCTGA CCAGAAGCAC CAGCCAGGAC TCAGAGGCCA GCACAGTGGT AAGTAACAGC
    TCAGGTGAGA CGCTCGGTGC AGACAGTGAC CTGAGCAGCA CTGCTGGAGA TGGCCTGACA
    GGAAGACATG GTCAGCATCT CAACCTGTCC CGTGGCACAC TGTCTGACAG TGAGATTGAG
    ACCAACCCTG CAACCAGCTC AGTGTTTGGC AAGACACACA AGCTGAAGCC AGGAGTGAAG
    GAGCCTGTTG GTGTTAATAA AGCAGCACCT GCTCCACCCG TTGAGGATGT TAGCATGAGG
    ATCTATCTGT GTGAGGGGCT TCTGGGCCGG GATAAAAGCT CAGTTTGGGA CCAGTTGGAG
    GATGCTGCAA TGGAAACCTT TTCTCTGAGT AAAGAGCGCT CAACTCTGTG GGATCAGATG
    CAGTTCTGGG AGGACGCCTT CCTGGATGCC GTAATGCTGG AACGGGAAGG AATGGGAATG
    GATCAGGGAC CACAAGAAAT GATTGACAGG TATCTTTCTC TGGGAGATCA TGACCGCAAG
    AGACTGGAGG ATGATGAGGA CAGACTGCTG GCCACGCTTC TGCACAATAT GATTGCTTAC
    ATGCTTATGA TGAAGGTCGG TAAAAACGAC ATCAGGAAGA AAGTACGTCG TCTTATGGGC
    AAATCCCACA TTGGCCTCAC TCACAGTCAG GAGATCAATG AAGTCCTGGA TCGCCTCGCA
    CATTTAAGCG GTCGGGAGCT TTCTATCAGG CCGAGTGGCA GCAGGCATAT TAAAAAACAG
    ACATTTGTTG TGCATGCTGG GACAGATACA ACTGGTGACA TATTCTTCAT GGAGGTGTGT
    GATGATTGCA TCGTCTTGCG CAGTAATATT GGCACAGTCT ATGAGCGCTG GTGGTATGAG
    AAGCTCATAA ATATGACTTA CTGTCCCAAA ACTAAAGTGT TGTGTCTGTG GAGGAGAAAC
    GGCCAGGAGA CGCAGCTAAA CAAGTTTTAC ACTAAGAAGT GTCGGGAATT GTACTACTGT
    GTTAAAGACA GCATGGAGCG TGCTGCTGCT CGACAACAAA GCATCAAACC AGGTCCAGAG
    CTTGGAGGCG AGTTTCCGGT TCAGGACATG AAGACAGGGG AGGGTGGTCT CCTCCAAGTC
    ACACTGGAAG GCATCAACCT CAAATTTATG CACAGCCAGG TTTTCATAGA GCTGAATCAC
    ATTAAAAAGT GCAATACAGT GAAAGGAGTC TTTGTCCTGG AGGAGTTTGT TCCTGAAACT
    AAAGAAGTGG TGATCCATAA GTACAAGACT CCTATGGCAC ATCAGATCTG TTACTCGGTG
    CTCTGCCTCT TCTCCTACAT GGCGGCGGTC AAGGGGAAAG AGTCAGAGGG CAAACCCAAG
    ATGCTGTCCC CTCGTCCACT AGCGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144915
    Clone ID Related Accession (Same CDS sequence) XM_021471987.1
    Accession Version XM_021471987.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4803bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X34
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394698.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGACTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGGA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCGGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTTCCCAA GCTCTGCTCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTCCC CAGCCTCAAA GTATTTGGGC TAAATTCTCT AATGGAGATT
    GTAACAGAGA TCGGCCCGGG GAGCGGAGAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGAATTGTGG AGCAAGCACC AGGATAACAG AAAGCAAAAA
    GCTTTGGAAA AAGAACAGAG TGGTTTAAAT AAACGGAAAT CAGGAGCGGA TGGAGTAAAA
    CAACGTGTAG AAGGAGGAGA CACTGAGGAG AAGAAATCAC AGATCAGTGC AGACAGTGGC
    CTCAGTGTGA CATCTGGCTC ACAGAAGAGT GATACGGATT CCCTGGCGAG CTCTGAACCT
    CCTGCTCTGA CCAGAAGCAC CAGCCAGGAC TCAGAGGCCA GCACAGTGGT AAGTAACAGC
    TCAGGTGAGA CGCTCGGTGC AGACAGTGAC CTGAGCAGCA CTGCTGGAGA TGGCCTGACA
    GGAAGACATG GTCAGCATCT CAACCTGTCC CGTGGCACAC TGTCTGACAG TGAGATTGAG
    ACCAACCCTG CAACCAGCTC AGTGTTTGGC AAGACACACA AGCTGAAGCC AGGAGTGAAG
    GAGCCTGTTG GTGTTAATAA AGCAGCACCT GCTCCACCCG TTGAGGATGT TAGCATGAGG
    ATCTATCTGT GTGAGGGGCT TCTGGGCCGG GATAAAAGCT CAGTTTGGGA CCAGTTGGAG
    GATGCTGCAA TGGAAACCTT TTCTCTGAGT AAAGAGCGCT CAACTCTGTG GGATCAGATG
    CAGTTCTGGG AGGACGCCTT CCTGGATGCC GTAATGCTGG AACGGGAAGG AATGGGAATG
    GATCAGGGAC CACAAGAAAT GATTGACAGG TATCTTTCTC TGGGAGATCA TGACCGCAAG
    AGACTGGAGG ATGATGAGGA CAGACTGCTG GCCACGCTTC TGCACAATAT GATTGCTTAC
    ATGCTTATGA TGAAGGTCGG TAAAAACGAC ATCAGGAAGA AAGTACGTCG TCTTATGGGC
    AAATCCCACA TTGGCCTCAC TCACAGTCAG GAGATCAATG AAGTCCTGGA TCGCCTCGCA
    CATTTAAGCG GTCGGGAGCT TTCTATCAGG CCGAGTGGCA GCAGGCATAT TAAAAAACAG
    ACATTTGTTG TGCATGCTGG GACAGATACA ACTGGTGACA TATTCTTCAT GGAGGTGTGT
    GATGATTGCA TCGTCTTGCG CAGTAATATT GGCACAGTCT ATGAGCGCTG GTGGTATGAG
    AAGCTCATAA ATATGACTTA CTGTCCCAAA ACTAAAGTGT TGTGTCTGTG GAGGAGAAAC
    GGCCAGGAGA CGCAGCTAAA CAAGTTTTAC ACTAAGAAGT GTCGGGAATT GTACTACTGT
    GTTAAAGACA GCATGGAGCG TGCTGCTGCT CGACAACAAA GCATCAAACC AGGTCCAGAG
    CTTGGAGGCG AGTTTCCGGT TCAGGACATG AAGACAGGGG AGGGTGGTCT CCTCCAAGTC
    ACACTGGAAG GCATCAACCT CAAATTTATG CACAGCCAGT TCCTGAAACT AAAGAAGTGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327662.1
    CDS169..4971
    Translation

    Target ORF information:

    RefSeq Version XM_021471987.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X69, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471987.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGACTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGGA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCGGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTTCCCAA GCTCTGCTCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTCCC CAGCCTCAAA GTATTTGGGC TAAATTCTCT AATGGAGATT
    GTAACAGAGA TCGGCCCGGG GAGCGGAGAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGAATTGTGG AGCAAGCACC AGGATAACAG AAAGCAAAAA
    GCTTTGGAAA AAGAACAGAG TGGTTTAAAT AAACGGAAAT CAGGAGCGGA TGGAGTAAAA
    CAACGTGTAG AAGGAGGAGA CACTGAGGAG AAGAAATCAC AGATCAGTGC AGACAGTGGC
    CTCAGTGTGA CATCTGGCTC ACAGAAGAGT GATACGGATT CCCTGGCGAG CTCTGAACCT
    CCTGCTCTGA CCAGAAGCAC CAGCCAGGAC TCAGAGGCCA GCACAGTGGT AAGTAACAGC
    TCAGGTGAGA CGCTCGGTGC AGACAGTGAC CTGAGCAGCA CTGCTGGAGA TGGCCTGACA
    GGAAGACATG GTCAGCATCT CAACCTGTCC CGTGGCACAC TGTCTGACAG TGAGATTGAG
    ACCAACCCTG CAACCAGCTC AGTGTTTGGC AAGACACACA AGCTGAAGCC AGGAGTGAAG
    GAGCCTGTTG GTGTTAATAA AGCAGCACCT GCTCCACCCG TTGAGGATGT TAGCATGAGG
    ATCTATCTGT GTGAGGGGCT TCTGGGCCGG GATAAAAGCT CAGTTTGGGA CCAGTTGGAG
    GATGCTGCAA TGGAAACCTT TTCTCTGAGT AAAGAGCGCT CAACTCTGTG GGATCAGATG
    CAGTTCTGGG AGGACGCCTT CCTGGATGCC GTAATGCTGG AACGGGAAGG AATGGGAATG
    GATCAGGGAC CACAAGAAAT GATTGACAGG TATCTTTCTC TGGGAGATCA TGACCGCAAG
    AGACTGGAGG ATGATGAGGA CAGACTGCTG GCCACGCTTC TGCACAATAT GATTGCTTAC
    ATGCTTATGA TGAAGGTCGG TAAAAACGAC ATCAGGAAGA AAGTACGTCG TCTTATGGGC
    AAATCCCACA TTGGCCTCAC TCACAGTCAG GAGATCAATG AAGTCCTGGA TCGCCTCGCA
    CATTTAAGCG GTCGGGAGCT TTCTATCAGG CCGAGTGGCA GCAGGCATAT TAAAAAACAG
    ACATTTGTTG TGCATGCTGG GACAGATACA ACTGGTGACA TATTCTTCAT GGAGGTGTGT
    GATGATTGCA TCGTCTTGCG CAGTAATATT GGCACAGTCT ATGAGCGCTG GTGGTATGAG
    AAGCTCATAA ATATGACTTA CTGTCCCAAA ACTAAAGTGT TGTGTCTGTG GAGGAGAAAC
    GGCCAGGAGA CGCAGCTAAA CAAGTTTTAC ACTAAGAAGT GTCGGGAATT GTACTACTGT
    GTTAAAGACA GCATGGAGCG TGCTGCTGCT CGACAACAAA GCATCAAACC AGGTCCAGAG
    CTTGGAGGCG AGTTTCCGGT TCAGGACATG AAGACAGGGG AGGGTGGTCT CCTCCAAGTC
    ACACTGGAAG GCATCAACCT CAAATTTATG CACAGCCAGT TCCTGAAACT AAAGAAGTGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144916
    Clone ID Related Accession (Same CDS sequence) XM_021471988.1
    Accession Version XM_021471988.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4800bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X35
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394698.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGACTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGGA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCGGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTTCCCAA GCTCTGCTCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTCCC CAGCCTCAAA GTATTTGGGC TAAATTCTCT AATGGAGATT
    GTAACAGAGA TCGGCCCGGG GAGCGGAGAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGAATTGTGG AGCAAGCACC AGGATAACAG AAAGCAAAAA
    GCTTTGGAAA AAGAACAGAG TGGTTTAAAT AAACGGAAAT CAGGAGCGGA TGGAGTAAAA
    CAACGTGTAG AAGGAGGAGA CACTGAGGAG AAGAAATCAC AGATCAGTGC AGACAGTGGC
    CTCAGTGTGA CATCTGGCTC ACAGAAGAGT GATACGGATT CCCTGGCGAG CTCTGAACCT
    CCTGCTCTGA CCAGAAGCAC CAGCCAGGAC TCAGAGGCCA GCACAGTAAG TAACAGCTCA
    GGTGAGACGC TCGGTGCAGA CAGTGACCTG AGCAGCACTG CTGGAGATGG CCTGACAGGA
    AGACATGGTC AGCATCTCAA CCTGTCCCGT GGCACACTGT CTGACAGTGA GATTGAGACC
    AACCCTGCAA CCAGCTCAGT GTTTGGCAAG ACACACAAGC TGAAGCCAGG AGTGAAGGAG
    CCTGTTGGTG TTAATAAAGC AGCACCTGCT CCACCCGTTG AGGATGTTAG CATGAGGATC
    TATCTGTGTG AGGGGCTTCT GGGCCGGGAT AAAAGCTCAG TTTGGGACCA GTTGGAGGAT
    GCTGCAATGG AAACCTTTTC TCTGAGTAAA GAGCGCTCAA CTCTGTGGGA TCAGATGCAG
    TTCTGGGAGG ACGCCTTCCT GGATGCCGTA ATGCTGGAAC GGGAAGGAAT GGGAATGGAT
    CAGGGACCAC AAGAAATGAT TGACAGGTAT CTTTCTCTGG GAGATCATGA CCGCAAGAGA
    CTGGAGGATG ATGAGGACAG ACTGCTGGCC ACGCTTCTGC ACAATATGAT TGCTTACATG
    CTTATGATGA AGGTCGGTAA AAACGACATC AGGAAGAAAG TACGTCGTCT TATGGGCAAA
    TCCCACATTG GCCTCACTCA CAGTCAGGAG ATCAATGAAG TCCTGGATCG CCTCGCACAT
    TTAAGCGGTC GGGAGCTTTC TATCAGGCCG AGTGGCAGCA GGCATATTAA AAAACAGACA
    TTTGTTGTGC ATGCTGGGAC AGATACAACT GGTGACATAT TCTTCATGGA GGTGTGTGAT
    GATTGCATCG TCTTGCGCAG TAATATTGGC ACAGTCTATG AGCGCTGGTG GTATGAGAAG
    CTCATAAATA TGACTTACTG TCCCAAAACT AAAGTGTTGT GTCTGTGGAG GAGAAACGGC
    CAGGAGACGC AGCTAAACAA GTTTTACACT AAGAAGTGTC GGGAATTGTA CTACTGTGTT
    AAAGACAGCA TGGAGCGTGC TGCTGCTCGA CAACAAAGCA TCAAACCAGG TCCAGAGCTT
    GGAGGCGAGT TTCCGGTTCA GGACATGAAG ACAGGGGAGG GTGGTCTCCT CCAAGTCACA
    CTGGAAGGCA TCAACCTCAA ATTTATGCAC AGCCAGTTCC TGAAACTAAA GAAGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327663.1
    CDS169..4968
    Translation

    Target ORF information:

    RefSeq Version XM_021471988.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X70, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471988.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGACTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGGA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCGGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTTCCCAA GCTCTGCTCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTCCC CAGCCTCAAA GTATTTGGGC TAAATTCTCT AATGGAGATT
    GTAACAGAGA TCGGCCCGGG GAGCGGAGAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGAATTGTGG AGCAAGCACC AGGATAACAG AAAGCAAAAA
    GCTTTGGAAA AAGAACAGAG TGGTTTAAAT AAACGGAAAT CAGGAGCGGA TGGAGTAAAA
    CAACGTGTAG AAGGAGGAGA CACTGAGGAG AAGAAATCAC AGATCAGTGC AGACAGTGGC
    CTCAGTGTGA CATCTGGCTC ACAGAAGAGT GATACGGATT CCCTGGCGAG CTCTGAACCT
    CCTGCTCTGA CCAGAAGCAC CAGCCAGGAC TCAGAGGCCA GCACAGTAAG TAACAGCTCA
    GGTGAGACGC TCGGTGCAGA CAGTGACCTG AGCAGCACTG CTGGAGATGG CCTGACAGGA
    AGACATGGTC AGCATCTCAA CCTGTCCCGT GGCACACTGT CTGACAGTGA GATTGAGACC
    AACCCTGCAA CCAGCTCAGT GTTTGGCAAG ACACACAAGC TGAAGCCAGG AGTGAAGGAG
    CCTGTTGGTG TTAATAAAGC AGCACCTGCT CCACCCGTTG AGGATGTTAG CATGAGGATC
    TATCTGTGTG AGGGGCTTCT GGGCCGGGAT AAAAGCTCAG TTTGGGACCA GTTGGAGGAT
    GCTGCAATGG AAACCTTTTC TCTGAGTAAA GAGCGCTCAA CTCTGTGGGA TCAGATGCAG
    TTCTGGGAGG ACGCCTTCCT GGATGCCGTA ATGCTGGAAC GGGAAGGAAT GGGAATGGAT
    CAGGGACCAC AAGAAATGAT TGACAGGTAT CTTTCTCTGG GAGATCATGA CCGCAAGAGA
    CTGGAGGATG ATGAGGACAG ACTGCTGGCC ACGCTTCTGC ACAATATGAT TGCTTACATG
    CTTATGATGA AGGTCGGTAA AAACGACATC AGGAAGAAAG TACGTCGTCT TATGGGCAAA
    TCCCACATTG GCCTCACTCA CAGTCAGGAG ATCAATGAAG TCCTGGATCG CCTCGCACAT
    TTAAGCGGTC GGGAGCTTTC TATCAGGCCG AGTGGCAGCA GGCATATTAA AAAACAGACA
    TTTGTTGTGC ATGCTGGGAC AGATACAACT GGTGACATAT TCTTCATGGA GGTGTGTGAT
    GATTGCATCG TCTTGCGCAG TAATATTGGC ACAGTCTATG AGCGCTGGTG GTATGAGAAG
    CTCATAAATA TGACTTACTG TCCCAAAACT AAAGTGTTGT GTCTGTGGAG GAGAAACGGC
    CAGGAGACGC AGCTAAACAA GTTTTACACT AAGAAGTGTC GGGAATTGTA CTACTGTGTT
    AAAGACAGCA TGGAGCGTGC TGCTGCTCGA CAACAAAGCA TCAAACCAGG TCCAGAGCTT
    GGAGGCGAGT TTCCGGTTCA GGACATGAAG ACAGGGGAGG GTGGTCTCCT CCAAGTCACA
    CTGGAAGGCA TCAACCTCAA ATTTATGCAC AGCCAGTTCC TGAAACTAAA GAAGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144917
    Clone ID Related Accession (Same CDS sequence) XM_021471989.1
    Accession Version XM_021471989.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4743bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X36
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394698.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGACTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGGA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCGGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTTCCCAA GCTCTGCTCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTCCC CAGCCTCAAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGAATTGTGG AGCAAGCACC AGGATAACAG AAAGCAAAAA
    GCTTTGGAAA AAGAACAGAG TGGTTTAAAT AAACGGAAAT CAGGAGCGGA TGGAGTAAAA
    CAACGTGTAG AAGGAGGAGA CACTGAGGAG AAGAAATCAC AGATCAGTGC AGACAGTGGC
    CTCAGTGTGA CATCTGGCTC ACAGAAGAGT GATACGGATT CCCTGGCGAG CTCTGAACCT
    CCTGCTCTGA CCAGAAGCAC CAGCCAGGAC TCAGAGGCCA GCACAGTGGT AAGTAACAGC
    TCAGGTGAGA CGCTCGGTGC AGACAGTGAC CTGAGCAGCA CTGCTGGAGA TGGCCTGACA
    GGAAGACATG GTCAGCATCT CAACCTGTCC CGTGGCACAC TGTCTGACAG TGAGATTGAG
    ACCAACCCTG CAACCAGCTC AGTGTTTGGC AAGACACACA AGCTGAAGCC AGGAGTGAAG
    GAGCCTGTTG GTGTTAATAA AGCAGCACCT GCTCCACCCG TTGAGGATGT TAGCATGAGG
    ATCTATCTGT GTGAGGGGCT TCTGGGCCGG GATAAAAGCT CAGTTTGGGA CCAGTTGGAG
    GATGCTGCAA TGGAAACCTT TTCTCTGAGT AAAGAGCGCT CAACTCTGTG GGATCAGATG
    CAGTTCTGGG AGGACGCCTT CCTGGATGCC GTAATGCTGG AACGGGAAGG AATGGGAATG
    GATCAGGGAC CACAAGAAAT GATTGACAGG TATCTTTCTC TGGGAGATCA TGACCGCAAG
    AGACTGGAGG ATGATGAGGA CAGACTGCTG GCCACGCTTC TGCACAATAT GATTGCTTAC
    ATGCTTATGA TGAAGGTCGG TAAAAACGAC ATCAGGAAGA AAGTACGTCG TCTTATGGGC
    AAATCCCACA TTGGCCTCAC TCACAGTCAG GAGATCAATG AAGTCCTGGA TCGCCTCGCA
    CATTTAAGCG GTCGGGAGCT TTCTATCAGG CCGAGTGGCA GCAGGCATAT TAAAAAACAG
    ACATTTGTTG TGCATGCTGG GACAGATACA ACTGGTGACA TATTCTTCAT GGAGGTGTGT
    GATGATTGCA TCGTCTTGCG CAGTAATATT GGCACAGTCT ATGAGCGCTG GTGGTATGAG
    AAGCTCATAA ATATGACTTA CTGTCCCAAA ACTAAAGTGT TGTGTCTGTG GAGGAGAAAC
    GGCCAGGAGA CGCAGCTAAA CAAGTTTTAC ACTAAGAAGT GTCGGGAATT GTACTACTGT
    GTTAAAGACA GCATGGAGCG TGCTGCTGCT CGACAACAAA GCATCAAACC AGGTCCAGAG
    CTTGGAGGCG AGTTTCCGGT TCAGGACATG AAGACAGGGG AGGGTGGTCT CCTCCAAGTC
    ACACTGGAAG GCATCAACCT CAAATTTATG CACAGCCAGT TCCTGAAACT AAAGAAGTGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327664.1
    CDS169..4911
    Translation

    Target ORF information:

    RefSeq Version XM_021471989.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X71, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471989.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGACTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGGA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCGGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTTCCCAA GCTCTGCTCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTCCC CAGCCTCAAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGAATTGTGG AGCAAGCACC AGGATAACAG AAAGCAAAAA
    GCTTTGGAAA AAGAACAGAG TGGTTTAAAT AAACGGAAAT CAGGAGCGGA TGGAGTAAAA
    CAACGTGTAG AAGGAGGAGA CACTGAGGAG AAGAAATCAC AGATCAGTGC AGACAGTGGC
    CTCAGTGTGA CATCTGGCTC ACAGAAGAGT GATACGGATT CCCTGGCGAG CTCTGAACCT
    CCTGCTCTGA CCAGAAGCAC CAGCCAGGAC TCAGAGGCCA GCACAGTGGT AAGTAACAGC
    TCAGGTGAGA CGCTCGGTGC AGACAGTGAC CTGAGCAGCA CTGCTGGAGA TGGCCTGACA
    GGAAGACATG GTCAGCATCT CAACCTGTCC CGTGGCACAC TGTCTGACAG TGAGATTGAG
    ACCAACCCTG CAACCAGCTC AGTGTTTGGC AAGACACACA AGCTGAAGCC AGGAGTGAAG
    GAGCCTGTTG GTGTTAATAA AGCAGCACCT GCTCCACCCG TTGAGGATGT TAGCATGAGG
    ATCTATCTGT GTGAGGGGCT TCTGGGCCGG GATAAAAGCT CAGTTTGGGA CCAGTTGGAG
    GATGCTGCAA TGGAAACCTT TTCTCTGAGT AAAGAGCGCT CAACTCTGTG GGATCAGATG
    CAGTTCTGGG AGGACGCCTT CCTGGATGCC GTAATGCTGG AACGGGAAGG AATGGGAATG
    GATCAGGGAC CACAAGAAAT GATTGACAGG TATCTTTCTC TGGGAGATCA TGACCGCAAG
    AGACTGGAGG ATGATGAGGA CAGACTGCTG GCCACGCTTC TGCACAATAT GATTGCTTAC
    ATGCTTATGA TGAAGGTCGG TAAAAACGAC ATCAGGAAGA AAGTACGTCG TCTTATGGGC
    AAATCCCACA TTGGCCTCAC TCACAGTCAG GAGATCAATG AAGTCCTGGA TCGCCTCGCA
    CATTTAAGCG GTCGGGAGCT TTCTATCAGG CCGAGTGGCA GCAGGCATAT TAAAAAACAG
    ACATTTGTTG TGCATGCTGG GACAGATACA ACTGGTGACA TATTCTTCAT GGAGGTGTGT
    GATGATTGCA TCGTCTTGCG CAGTAATATT GGCACAGTCT ATGAGCGCTG GTGGTATGAG
    AAGCTCATAA ATATGACTTA CTGTCCCAAA ACTAAAGTGT TGTGTCTGTG GAGGAGAAAC
    GGCCAGGAGA CGCAGCTAAA CAAGTTTTAC ACTAAGAAGT GTCGGGAATT GTACTACTGT
    GTTAAAGACA GCATGGAGCG TGCTGCTGCT CGACAACAAA GCATCAAACC AGGTCCAGAG
    CTTGGAGGCG AGTTTCCGGT TCAGGACATG AAGACAGGGG AGGGTGGTCT CCTCCAAGTC
    ACACTGGAAG GCATCAACCT CAAATTTATG CACAGCCAGT TCCTGAAACT AAAGAAGTGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144918
    Clone ID Related Accession (Same CDS sequence) XM_021471990.1
    Accession Version XM_021471990.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4869bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X37
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394698.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGACTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGGA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCGGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTTCCCAA GCTCTGCTCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTCCC CAGCCTCAAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGGAGCGGAT GGAGTAAAAC AACGTGTAGA AGGAGGAGAC
    ACTGAGGAGA AGAAATCACA GATCAGTGCA GACAGTGGCC TCAGTGTGAC ATCTGGCTCA
    CAGAAGAGTG ATACGGATTC CCTGGCGAGC TCTGAACCTC CTGCTCTGAC CAGAAGCACC
    AGCCAGGACT CAGAGGCCAG CACAGTGGTA AGTAACAGCT CAGGTGAGAC GCTCGGTGCA
    GACAGTGACC TGAGCAGCAC TGCTGGAGAT GGCCTGACAG GAAGACATGG TCAGCATCTC
    AACCTGTCCC GTGGCACACT GTCTGACAGT GAGATTGAGA CCAACCCTGC AACCAGCTCA
    GTGTTTGGCA AGACACACAA GCTGAAGCCA GGAGTGAAGG AGCCTGTTGG TGTTAATAAA
    GCAGCACCTG CTCCACCCGT TGAGGATGTT AGCATGAGGA TCTATCTGTG TGAGGGGCTT
    CTGGGCCGGG ATAAAAGCTC AGTTTGGGAC CAGTTGGAGG ATGCTGCAAT GGAAACCTTT
    TCTCTGAGTA AAGAGCGCTC AACTCTGTGG GATCAGATGC AGTTCTGGGA GGACGCCTTC
    CTGGATGCCG TAATGCTGGA ACGGGAAGGA ATGGGAATGG ATCAGGGACC ACAAGAAATG
    ATTGACAGGT ATCTTTCTCT GGGAGATCAT GACCGCAAGA GACTGGAGGA TGATGAGGAC
    AGACTGCTGG CCACGCTTCT GCACAATATG ATTGCTTACA TGCTTATGAT GAAGGTCGGT
    AAAAACGACA TCAGGAAGAA AGTACGTCGT CTTATGGGCA AATCCCACAT TGGCCTCACT
    CACAGTCAGG AGATCAATGA AGTCCTGGAT CGCCTCGCAC ATTTAAGCGG TCGGGAGCTT
    TCTATCAGGC CGAGTGGCAG CAGGCATATT AAAAAACAGA CATTTGTTGT GCATGCTGGG
    ACAGATACAA CTGGTGACAT ATTCTTCATG GAGGTGTGTG ATGATTGCAT CGTCTTGCGC
    AGTAATATTG GCACAGTCTA TGAGCGCTGG TGGTATGAGA AGCTCATAAA TATGACTTAC
    TGTCCCAAAA CTAAAGTGTT GTGTCTGTGG AGGAGAAACG GCCAGGAGAC GCAGCTAAAC
    AAGTTTTACA CTAAGAAGTG TCGGGAATTG TACTACTGTG TTAAAGACAG CATGGAGCGT
    GCTGCTGCTC GACAACAAAG CATCAAACCA GGTCCAGAGC TTGGAGGCGA GTTTCCGGTT
    CAGGACATGA AGACAGGGGA GGGTGGTCTC CTCCAAGTCA CACTGGAAGG CATCAACCTC
    AAATTTATGC ACAGCCAGGT TTTCATAGAG CTGAATCACA TTAAAAAGTG CAATACAGTG
    AAAGGAGTCT TTGTCCTGGA GGAGTTTGTT CCTGAAACTA AAGAAGTGGT GATCCATAAG
    TACAAGACTC CTATGGCACA TCAGATCTGT TACTCGGTGC TCTGCCTCTT CTCCTACATG
    GCGGCGGTCA AGGGGAAAGA GTCAGAGGGC AAACCCAAGA TGCTGTCCCC TCGTCCACTA
    GCGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327665.1
    CDS169..5037
    Translation

    Target ORF information:

    RefSeq Version XM_021471990.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X72, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471990.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGACTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGGA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCGGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTTCCCAA GCTCTGCTCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTCCC CAGCCTCAAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGGAGCGGAT GGAGTAAAAC AACGTGTAGA AGGAGGAGAC
    ACTGAGGAGA AGAAATCACA GATCAGTGCA GACAGTGGCC TCAGTGTGAC ATCTGGCTCA
    CAGAAGAGTG ATACGGATTC CCTGGCGAGC TCTGAACCTC CTGCTCTGAC CAGAAGCACC
    AGCCAGGACT CAGAGGCCAG CACAGTGGTA AGTAACAGCT CAGGTGAGAC GCTCGGTGCA
    GACAGTGACC TGAGCAGCAC TGCTGGAGAT GGCCTGACAG GAAGACATGG TCAGCATCTC
    AACCTGTCCC GTGGCACACT GTCTGACAGT GAGATTGAGA CCAACCCTGC AACCAGCTCA
    GTGTTTGGCA AGACACACAA GCTGAAGCCA GGAGTGAAGG AGCCTGTTGG TGTTAATAAA
    GCAGCACCTG CTCCACCCGT TGAGGATGTT AGCATGAGGA TCTATCTGTG TGAGGGGCTT
    CTGGGCCGGG ATAAAAGCTC AGTTTGGGAC CAGTTGGAGG ATGCTGCAAT GGAAACCTTT
    TCTCTGAGTA AAGAGCGCTC AACTCTGTGG GATCAGATGC AGTTCTGGGA GGACGCCTTC
    CTGGATGCCG TAATGCTGGA ACGGGAAGGA ATGGGAATGG ATCAGGGACC ACAAGAAATG
    ATTGACAGGT ATCTTTCTCT GGGAGATCAT GACCGCAAGA GACTGGAGGA TGATGAGGAC
    AGACTGCTGG CCACGCTTCT GCACAATATG ATTGCTTACA TGCTTATGAT GAAGGTCGGT
    AAAAACGACA TCAGGAAGAA AGTACGTCGT CTTATGGGCA AATCCCACAT TGGCCTCACT
    CACAGTCAGG AGATCAATGA AGTCCTGGAT CGCCTCGCAC ATTTAAGCGG TCGGGAGCTT
    TCTATCAGGC CGAGTGGCAG CAGGCATATT AAAAAACAGA CATTTGTTGT GCATGCTGGG
    ACAGATACAA CTGGTGACAT ATTCTTCATG GAGGTGTGTG ATGATTGCAT CGTCTTGCGC
    AGTAATATTG GCACAGTCTA TGAGCGCTGG TGGTATGAGA AGCTCATAAA TATGACTTAC
    TGTCCCAAAA CTAAAGTGTT GTGTCTGTGG AGGAGAAACG GCCAGGAGAC GCAGCTAAAC
    AAGTTTTACA CTAAGAAGTG TCGGGAATTG TACTACTGTG TTAAAGACAG CATGGAGCGT
    GCTGCTGCTC GACAACAAAG CATCAAACCA GGTCCAGAGC TTGGAGGCGA GTTTCCGGTT
    CAGGACATGA AGACAGGGGA GGGTGGTCTC CTCCAAGTCA CACTGGAAGG CATCAACCTC
    AAATTTATGC ACAGCCAGGT TTTCATAGAG CTGAATCACA TTAAAAAGTG CAATACAGTG
    AAAGGAGTCT TTGTCCTGGA GGAGTTTGTT CCTGAAACTA AAGAAGTGGT GATCCATAAG
    TACAAGACTC CTATGGCACA TCAGATCTGT TACTCGGTGC TCTGCCTCTT CTCCTACATG
    GCGGCGGTCA AGGGGAAAGA GTCAGAGGGC AAACCCAAGA TGCTGTCCCC TCGTCCACTA
    GCGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144919
    Clone ID Related Accession (Same CDS sequence) XM_021471991.1
    Accession Version XM_021471991.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4599bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X38
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394698.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGACTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGGA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCGGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTTCCCAA GCTCTGCTCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTCCC CAGCCTCAAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGGAGCGGAT GGAGTAAAAC AACGTGTAGA AGGAGGAGAC
    ACTGAGGAGA AGAAATCACA GATCAGTGCA GACAGTGGCC TCAGTGTGAC ATCTGGCTCA
    CAGAAGAGTG ATACGGATTC CCTGGCGAGC TCTGAACCTC CTGCTCTGAC CAGAAGCACC
    AGCCAGGACT CAGAGGCCAG CACAGTGGTA AGTAACAGCT CAGGTGAGAC GCTCGGTGCA
    GACAGTGACC TGAGCAGCAC TGCTGGAGAT GGCCTGACAG GAAGACATGG TCAGCATCTC
    AACCTGTCCC GTGGCACACT GTCTGACAGT GAGATTGAGA CCAACCCTGC AACCAGCTCA
    GTGTTTGGCA AGACACACAA GCTGAAGCCA GGAGTGAAGG AGCCTGTTGG TGTTAATAAA
    GCAGCACCTG CTCCACCCGT TGAGGATGTT AGCATGAGGA TCTATCTGTG TGAGGGGCTT
    CTGGGTAAAG AGCGCTCAAC TCTGTGGGAT CAGATGCAGT TCTGGGAGGA CGCCTTCCTG
    GATGCCGTAA TGCTGGAACG GGAAGGAATG GGAATGGATC AGGGACCACA AGAAATGATT
    GACAGGTATC TTTCTCTGGG AGATCATGAC CGCAAGAGAC TGGAGGATGA TGAGGACAGA
    CTGCTGGCCA CGCTTCTGCA CAATATGATT GCTTACATGC TTATGATGAA GGTCGGTAAA
    AACGACATCA GGAAGAAAGT ACGTCGTCTT ATGGGCAAAT CCCACATTGG CCTCACTCAC
    AGTCAGGAGA TCAATGAAGT CCTGGATCGC CTCGCACATT TAAGCGGTCG GGAGCTTTCT
    ATCAGGCCGA GTGGCAGCAG GCATATTAAA AAACAGACAT TTGTTGTGCA TGCTGGGACA
    GATACAACTG GTGACATATT CTTCATGGAG GTGTGTGATG ATTGCATCGT CTTGCGCAGT
    AATATTGGCA CAGTCTATGA GCGCTGGTGG TATGAGAAGC TCATAAATAT GACTTACTGT
    CCCAAAACTA AAGTGTTGTG TCTGTGGAGG AGAAACGGCC AGGAGACGCA GCTAAACAAG
    TTTTACACTA AGAAGTGTCG GGAATTGTAC TACTGTGTTA AAGACAGCAT GGAGCGTGCT
    GCTGCTCGAC AACAAAGCAT CAAACCAGGT CCAGAGCTTG GAGGCGAGTT TCCGGTTCAG
    GACATGAAGA CAGGGGAGGG TGGTCTCCTC CAAGTCACAC TGGAAGGCAT CAACCTCAAA
    TTTATGCACA GCCAGTTCCT GAAACTAAAG AAGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327666.1
    CDS169..4767
    Translation

    Target ORF information:

    RefSeq Version XM_021471991.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X73, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471991.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTTACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAACTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTTTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TGGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCCTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGACTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGGA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCGGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTTCCCAA GCTCTGCTCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTCCC CAGCCTCAAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGGAGCGGAT GGAGTAAAAC AACGTGTAGA AGGAGGAGAC
    ACTGAGGAGA AGAAATCACA GATCAGTGCA GACAGTGGCC TCAGTGTGAC ATCTGGCTCA
    CAGAAGAGTG ATACGGATTC CCTGGCGAGC TCTGAACCTC CTGCTCTGAC CAGAAGCACC
    AGCCAGGACT CAGAGGCCAG CACAGTGGTA AGTAACAGCT CAGGTGAGAC GCTCGGTGCA
    GACAGTGACC TGAGCAGCAC TGCTGGAGAT GGCCTGACAG GAAGACATGG TCAGCATCTC
    AACCTGTCCC GTGGCACACT GTCTGACAGT GAGATTGAGA CCAACCCTGC AACCAGCTCA
    GTGTTTGGCA AGACACACAA GCTGAAGCCA GGAGTGAAGG AGCCTGTTGG TGTTAATAAA
    GCAGCACCTG CTCCACCCGT TGAGGATGTT AGCATGAGGA TCTATCTGTG TGAGGGGCTT
    CTGGGTAAAG AGCGCTCAAC TCTGTGGGAT CAGATGCAGT TCTGGGAGGA CGCCTTCCTG
    GATGCCGTAA TGCTGGAACG GGAAGGAATG GGAATGGATC AGGGACCACA AGAAATGATT
    GACAGGTATC TTTCTCTGGG AGATCATGAC CGCAAGAGAC TGGAGGATGA TGAGGACAGA
    CTGCTGGCCA CGCTTCTGCA CAATATGATT GCTTACATGC TTATGATGAA GGTCGGTAAA
    AACGACATCA GGAAGAAAGT ACGTCGTCTT ATGGGCAAAT CCCACATTGG CCTCACTCAC
    AGTCAGGAGA TCAATGAAGT CCTGGATCGC CTCGCACATT TAAGCGGTCG GGAGCTTTCT
    ATCAGGCCGA GTGGCAGCAG GCATATTAAA AAACAGACAT TTGTTGTGCA TGCTGGGACA
    GATACAACTG GTGACATATT CTTCATGGAG GTGTGTGATG ATTGCATCGT CTTGCGCAGT
    AATATTGGCA CAGTCTATGA GCGCTGGTGG TATGAGAAGC TCATAAATAT GACTTACTGT
    CCCAAAACTA AAGTGTTGTG TCTGTGGAGG AGAAACGGCC AGGAGACGCA GCTAAACAAG
    TTTTACACTA AGAAGTGTCG GGAATTGTAC TACTGTGTTA AAGACAGCAT GGAGCGTGCT
    GCTGCTCGAC AACAAAGCAT CAAACCAGGT CCAGAGCTTG GAGGCGAGTT TCCGGTTCAG
    GACATGAAGA CAGGGGAGGG TGGTCTCCTC CAAGTCACAC TGGAAGGCAT CAACCTCAAA
    TTTATGCACA GCCAGTTCCT GAAACTAAAG AAGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa59767
    Clone ID Related Accession (Same CDS sequence) XM_017357062.1
    Accession Version XM_017357062.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5103bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product MAP kinase-activating death domain protein isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGTG TCTGGTAAGG
    TTGACTCTGA TCACCCAAAA GCAGATCTTC GCCTCCGATT CTAAGGCTCT GGCTAGCATG
    AGTCTAAACA CACAGCCTAT TCTGAACCTG GAGAAGTTTC AGGATGGACA GGAGTTGTCT
    TTATTGCCCC CCGGTCGTGA CAAAGCTTCC CCGTCCTCCA CTGAGTTCAA CCCACTCATC
    TACGGCAATG ATGTGGACTC AGTTGATGTT GCCACAAGGG TGGCAATGGT GAGGTTCTTC
    AACTCTCCAA ACGTGCTGCA AGGGTTCCAG ATGCACACAC GCACTCTCCG TCTCTTCCCT
    CGTCCTGTGG TGGCCTTTCA GTCCACATCT TTCCTGGCTT CTAGGCCACG ACGAAATGGA
    TTCACAGAAA AGCTCTCCCA CACTCAGGCA GTGGAGTACT ATGGAGAATG GGCTCTCAAC
    CCCACCAACC TGGCTTTCCA AAGGATTCAT AACAATGTGT ATGACCCCTC TCTAATAGGC
    GATAAGCCTA AGTGGTATGC CCACCAGTTA CAGCCAGTAT TCTACCGCGT GTATGATGGA
    AACTCTCATT TAGCGGAGGC CTTGAGTGGA CCCCTGCAGG ATGAGACCAA TGATTCAGAC
    CCAACAGACG ACAGTGGCAG CGACAGTGAA GCATATGATG ACTCCAGCTC CTCCTACTCA
    TCTCTTGGAG ATTTTGTGAA TGAGATGATC AAAGGGGACA TACAGGGAGA CACACCAAAT
    GTTGATCCCC TGACCCATGC TGCACTGGGA GATGCAGATG AGGTTGAGAT CCATGACTTT
    CACGAGTATA AGGGGAACAG CGGTGAACCA GAGCCAGAAG GCCCCACAGA AGCAGCGGAC
    AGTCAGCCCT TGCGGTCCAG CTCTAGCACC ACAGCCAGTT CCAGCCCCAG TACAGTCATA
    CAGGGAGTCA ACCATGAGCA GAAGGAACCT GTTGAGGTGG AGCCCATAGC AAATGTGACT
    TACCCAAGCT CTGCCCCAGT ATTGGGTCCT CCTCCTTTTA CAAGACCCAC CCCTGATCCA
    ACCCTTGTTG ACCCTGCCAA TAAGAAGCGA GAATATGACA ACCCCTATTT TGAGCCCCAG
    TACGGTTTCC CTACAGAGGA AGATGCTGAG ACTGATGAGC AGGAGGAGAG TTACACACCA
    CGATTTAACC AGAATCTCAA TGGCAATAAG CCTTCTCGTC CACTGAGACC CAGCAGCCTG
    AAGCTTCCGG GTGAATCTGA CGGAGAGGGA GACTCCAGAA ATAGCTCTCC TAACTCAACC
    ATCTCCAACA ATAGCAGTGA TGGCTTTGGG GGACTCATGT CCTTTGCCAG TAACTTGTAT
    AAAAACCATG GCACCAGTTT CAGTCTGTCC AGCCTGGCTC TGCCTAATAA AGCGAGAGAG
    AAGAACACGC CGTTTCCCAG CCTCAAAGAC GATGCTGATG ACAGTCCTGA AAGTCCTGTA
    TTTGGGCTAA ATTCTCTAAT GGAGATTGTA ACAGAGATCG GCCCGGGGAG CGGAGAAGGT
    GCCCGAGCCC CCAGAGCACT GGTAGATCAG AAATCCTCTG TTATTAAGCA CAGCCCTACT
    GTGAAGAGAG AGTCTCCGTC TCCTCAAGGC AGAGCCAACA ATACCAGTGA GAACCAGCAG
    TTTCTAAAGG AGGTGGTCCA GAGCGTGCTG GAGGGGCAGG GTGTGGGCTG GCTTAACATG
    AAGAAGGTGC GTCGCCTGCT AGAGAATGAA CAGCTCAGAG TCTTTGTGCT CAGCAAACTC
    AACCGTGCGG TCCAGTCAGA GGAGGACGCC CAGCAGGAAG TTATAAGAGA TGTGGAGATA
    AATAGAAAGG TCTACAAAGG CATGCTGGAT ATTTTGAAGT GCACGGTGTC CAGTTTGGAG
    CATTCATACA CTAATGCTGG CTTAGGGGGC ATGGCCAGTG TCTTCAGTCT GCTAGAGATA
    GCACGCACCC ACTACCAAAC CAAAGATCCA GAGAAGCGGA AACGGAGCCC CACCGAAGGG
    CTTAGCAGTC CAGGCAGTAA GGAGAGCCCA TCGGGCCGAA TGGAGAGTGC CAGGGCGGCA
    GGGGTGCTTC TGGTACCCCG TATCCAATTG CCGCCACCCT CCTCTGGGAA ATCATCACGC
    CAGTTTGATA CCCGGAGTCT GAACGAGGAG AATTTTATTG CCTCCATTGA ATTGTGGAGC
    AAGCACCAGG ATAACAGAAA GCAAAAAGCT TTGGAAAAAG AACAGAGTGG TTTAAATAAA
    CGGAAATCAG GAGCGGATGG AGTAAAACAA CGTGTAGAAG GAGGAGACAC TGAGGAGAAG
    AAATCACAGA TCAGTGCAGA CAGTGGCCTC AGTGTGACAT CTGGCTCACA GAAGAGTGAT
    ACGGATTCCC TGGCGAGCTC TGAACCTCCT GCTCTGACCA GAAGCACCAG CCAGGACTCA
    GAGGCCAGCA CAGTAAGTAA CAGCTCAGGT GAGACGCTCG GTGCAGACAG TGACCTGAGC
    AGCACTGCTG GAGATGGCCT GACAGGAAGA CATGGTCAGC ATCTCAACCT GTCCCGTGGC
    ACACTGTCTG ACAGTGAGAT TGAGACCAAC CCTGCAACCA GCTCAGTGTT TGGCAAGACA
    CACAAGCTGA AGCCAGGAGT GAAGGAGCCT GTTGGTGTTA ATAAAGCAGC ACCTGCTCCA
    CCCGTTGAGG ATGTTAGCAT GAGGATCTAT CTGTGTGAGG GGCTTCTGGG CCGGGATAAA
    AGCTCAGTTT GGGACCAGTT GGAGGATGCT GCAATGGAAA CCTTTTCTCT GAGTAAAGAG
    CGCTCAACTC TGTGGGATCA GATGCAGTTC TGGGAGGACG CCTTCCTGGA TGCCGTAATG
    CTGGAACGGG AAGGAATGGG AATGGATCAG GGACCACAAG AAATGATTGA CAGGTATCTT
    TCTCTGGGAG ATCATGACCG CAAGAGACTG GAGGATGATG AGGACAGACT GCTGGCCACG
    CTTCTGCACA ATATGATTGC TTACATGCTT ATGATGAAGG TCGGTAAAAA CGACATCAGG
    AAGAAAGTAC GTCGTCTTAT GGGCAAATCC CACATTGGCC TCACTCACAG TCAGGAGATC
    AATGAAGTCC TGGATCGCCT CGCACATTTA AGCGGTCGGG AGCTTTCTAT CAGGCCGAGT
    GGCAGCAGGC ATATTAAAAA ACAGACATTT GTTGTGCATG CTGGGACAGA TACAACTGGT
    GACATATTCT TCATGGAGGT GTGTGATGAT TGCATCGTCT TGCGCAGTAA TATTGGCACA
    GTCTATGAGC GCTGGTGGTA TGAGAAGCTC ATAAATATGA CTTACTGTCC CAAAACTAAA
    GTGTTGTGTC TGTGGAGGAG AAACGGCCAG GAGACGCAGC TAAACAAGTT TTACACTAAG
    AAGTGTCGGG AATTGTACTA CTGTGTTAAA GACAGCATGG AGCGTGCTGC TGCTCGACAA
    CAAAGCATCA AACCAGGTCC AGAGCTTGGA GGCGAGTTTC CGGTTCAGGA CATGAAGACA
    GGGGAGGGTG GTCTCCTCCA AGTCACACTG GAAGGCATCA ACCTCAAATT TATGCACAGC
    CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT CACATTAAAA AGTGCAATAC AGTGAAAGGA
    GTCTTTGTCC TGGAGGAGTT TGTTCCTGAA ACTAAAGAAG TGGTGATCCA TAAGTACAAG
    ACTCCTATGG CACATCAGAT CTGTTACTCG GTGCTCTGCC TCTTCTCCTA CATGGCGGCG
    GTCAAGGGGA AAGAGTCAGA GGGCAAACCC AAGATGCTGT CCCCTCGTCC ACTAGCGAGC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017212551.1
    CDS435..5537
    Translation

    Target ORF information:

    RefSeq Version XM_017357062.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017357062.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGTG TCTGGTAAGG
    TTGACTCTGA TCACCCAAAA GCAGATCTTC GCCTCCGATT CTAAGGCTCT GGCTAGCATG
    AGTCTAAACA CACAGCCTAT TCTGAACCTG GAGAAGTTTC AGGATGGACA GGAGTTGTCT
    TTATTGCCCC CCGGTCGTGA CAAAGCTTCC CCGTCCTCCA CTGAGTTCAA CCCACTCATC
    TACGGCAATG ATGTGGACTC AGTTGATGTT GCCACAAGGG TGGCAATGGT GAGGTTCTTC
    AACTCTCCAA ACGTGCTGCA AGGGTTCCAG ATGCACACAC GCACTCTCCG TCTCTTCCCT
    CGTCCTGTGG TGGCCTTTCA GTCCACATCT TTCCTGGCTT CTAGGCCACG ACGAAATGGA
    TTCACAGAAA AGCTCTCCCA CACTCAGGCA GTGGAGTACT ATGGAGAATG GGCTCTCAAC
    CCCACCAACC TGGCTTTCCA AAGGATTCAT AACAATGTGT ATGACCCCTC TCTAATAGGC
    GATAAGCCTA AGTGGTATGC CCACCAGTTA CAGCCAGTAT TCTACCGCGT GTATGATGGA
    AACTCTCATT TAGCGGAGGC CTTGAGTGGA CCCCTGCAGG ATGAGACCAA TGATTCAGAC
    CCAACAGACG ACAGTGGCAG CGACAGTGAA GCATATGATG ACTCCAGCTC CTCCTACTCA
    TCTCTTGGAG ATTTTGTGAA TGAGATGATC AAAGGGGACA TACAGGGAGA CACACCAAAT
    GTTGATCCCC TGACCCATGC TGCACTGGGA GATGCAGATG AGGTTGAGAT CCATGACTTT
    CACGAGTATA AGGGGAACAG CGGTGAACCA GAGCCAGAAG GCCCCACAGA AGCAGCGGAC
    AGTCAGCCCT TGCGGTCCAG CTCTAGCACC ACAGCCAGTT CCAGCCCCAG TACAGTCATA
    CAGGGAGTCA ACCATGAGCA GAAGGAACCT GTTGAGGTGG AGCCCATAGC AAATGTGACT
    TACCCAAGCT CTGCCCCAGT ATTGGGTCCT CCTCCTTTTA CAAGACCCAC CCCTGATCCA
    ACCCTTGTTG ACCCTGCCAA TAAGAAGCGA GAATATGACA ACCCCTATTT TGAGCCCCAG
    TACGGTTTCC CTACAGAGGA AGATGCTGAG ACTGATGAGC AGGAGGAGAG TTACACACCA
    CGATTTAACC AGAATCTCAA TGGCAATAAG CCTTCTCGTC CACTGAGACC CAGCAGCCTG
    AAGCTTCCGG GTGAATCTGA CGGAGAGGGA GACTCCAGAA ATAGCTCTCC TAACTCAACC
    ATCTCCAACA ATAGCAGTGA TGGCTTTGGG GGACTCATGT CCTTTGCCAG TAACTTGTAT
    AAAAACCATG GCACCAGTTT CAGTCTGTCC AGCCTGGCTC TGCCTAATAA AGCGAGAGAG
    AAGAACACGC CGTTTCCCAG CCTCAAAGAC GATGCTGATG ACAGTCCTGA AAGTCCTGTA
    TTTGGGCTAA ATTCTCTAAT GGAGATTGTA ACAGAGATCG GCCCGGGGAG CGGAGAAGGT
    GCCCGAGCCC CCAGAGCACT GGTAGATCAG AAATCCTCTG TTATTAAGCA CAGCCCTACT
    GTGAAGAGAG AGTCTCCGTC TCCTCAAGGC AGAGCCAACA ATACCAGTGA GAACCAGCAG
    TTTCTAAAGG AGGTGGTCCA GAGCGTGCTG GAGGGGCAGG GTGTGGGCTG GCTTAACATG
    AAGAAGGTGC GTCGCCTGCT AGAGAATGAA CAGCTCAGAG TCTTTGTGCT CAGCAAACTC
    AACCGTGCGG TCCAGTCAGA GGAGGACGCC CAGCAGGAAG TTATAAGAGA TGTGGAGATA
    AATAGAAAGG TCTACAAAGG CATGCTGGAT ATTTTGAAGT GCACGGTGTC CAGTTTGGAG
    CATTCATACA CTAATGCTGG CTTAGGGGGC ATGGCCAGTG TCTTCAGTCT GCTAGAGATA
    GCACGCACCC ACTACCAAAC CAAAGATCCA GAGAAGCGGA AACGGAGCCC CACCGAAGGG
    CTTAGCAGTC CAGGCAGTAA GGAGAGCCCA TCGGGCCGAA TGGAGAGTGC CAGGGCGGCA
    GGGGTGCTTC TGGTACCCCG TATCCAATTG CCGCCACCCT CCTCTGGGAA ATCATCACGC
    CAGTTTGATA CCCGGAGTCT GAACGAGGAG AATTTTATTG CCTCCATTGA ATTGTGGAGC
    AAGCACCAGG ATAACAGAAA GCAAAAAGCT TTGGAAAAAG AACAGAGTGG TTTAAATAAA
    CGGAAATCAG GAGCGGATGG AGTAAAACAA CGTGTAGAAG GAGGAGACAC TGAGGAGAAG
    AAATCACAGA TCAGTGCAGA CAGTGGCCTC AGTGTGACAT CTGGCTCACA GAAGAGTGAT
    ACGGATTCCC TGGCGAGCTC TGAACCTCCT GCTCTGACCA GAAGCACCAG CCAGGACTCA
    GAGGCCAGCA CAGTAAGTAA CAGCTCAGGT GAGACGCTCG GTGCAGACAG TGACCTGAGC
    AGCACTGCTG GAGATGGCCT GACAGGAAGA CATGGTCAGC ATCTCAACCT GTCCCGTGGC
    ACACTGTCTG ACAGTGAGAT TGAGACCAAC CCTGCAACCA GCTCAGTGTT TGGCAAGACA
    CACAAGCTGA AGCCAGGAGT GAAGGAGCCT GTTGGTGTTA ATAAAGCAGC ACCTGCTCCA
    CCCGTTGAGG ATGTTAGCAT GAGGATCTAT CTGTGTGAGG GGCTTCTGGG CCGGGATAAA
    AGCTCAGTTT GGGACCAGTT GGAGGATGCT GCAATGGAAA CCTTTTCTCT GAGTAAAGAG
    CGCTCAACTC TGTGGGATCA GATGCAGTTC TGGGAGGACG CCTTCCTGGA TGCCGTAATG
    CTGGAACGGG AAGGAATGGG AATGGATCAG GGACCACAAG AAATGATTGA CAGGTATCTT
    TCTCTGGGAG ATCATGACCG CAAGAGACTG GAGGATGATG AGGACAGACT GCTGGCCACG
    CTTCTGCACA ATATGATTGC TTACATGCTT ATGATGAAGG TCGGTAAAAA CGACATCAGG
    AAGAAAGTAC GTCGTCTTAT GGGCAAATCC CACATTGGCC TCACTCACAG TCAGGAGATC
    AATGAAGTCC TGGATCGCCT CGCACATTTA AGCGGTCGGG AGCTTTCTAT CAGGCCGAGT
    GGCAGCAGGC ATATTAAAAA ACAGACATTT GTTGTGCATG CTGGGACAGA TACAACTGGT
    GACATATTCT TCATGGAGGT GTGTGATGAT TGCATCGTCT TGCGCAGTAA TATTGGCACA
    GTCTATGAGC GCTGGTGGTA TGAGAAGCTC ATAAATATGA CTTACTGTCC CAAAACTAAA
    GTGTTGTGTC TGTGGAGGAG AAACGGCCAG GAGACGCAGC TAAACAAGTT TTACACTAAG
    AAGTGTCGGG AATTGTACTA CTGTGTTAAA GACAGCATGG AGCGTGCTGC TGCTCGACAA
    CAAAGCATCA AACCAGGTCC AGAGCTTGGA GGCGAGTTTC CGGTTCAGGA CATGAAGACA
    GGGGAGGGTG GTCTCCTCCA AGTCACACTG GAAGGCATCA ACCTCAAATT TATGCACAGC
    CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT CACATTAAAA AGTGCAATAC AGTGAAAGGA
    GTCTTTGTCC TGGAGGAGTT TGTTCCTGAA ACTAAAGAAG TGGTGATCCA TAAGTACAAG
    ACTCCTATGG CACATCAGAT CTGTTACTCG GTGCTCTGCC TCTTCTCCTA CATGGCGGCG
    GTCAAGGGGA AAGAGTCAGA GGGCAAACCC AAGATGCTGT CCCCTCGTCC ACTAGCGAGC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa13173
    Clone ID Related Accession (Same CDS sequence) XM_009303285.2 , XM_021477902.1
    Accession Version XM_009303285.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4866bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product MAP kinase-activating death domain protein isoform X22
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303285.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GTATTTGGGC TAAATTCTCT AATGGAGATT
    GTAACAGAGA TCGGCCCGGG GAGCGGAGAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGGAGCGGAT GGAGTAAAAC AACGTGTAGA AGGAGGAGAC
    ACTGAGGAGA AGAAATCACA GATCAGTGCA GACAGTGGCC TCAGTGTGAC ATCTGGCTCA
    CAGAAGAGTG ATACGGATTC CCTGGCGAGC TCTGAACCTC CTGCTCTGAC CAGAAGCACC
    AGCCAGGACT CAGAGGCCAG CACAGTGGTA AGTAACAGCT CAGGTGAGAC GCTCGGTGCA
    GACAGTGACC TGAGCAGCAC TGCTGGAGAT GGCCTGACAG GAAGACATGG TCAGCATCTC
    AACCTGTCCC GTGGCACACT GTCTGACAGT GAGATTGAGA CCAACCCTGC AACCAGCTCA
    GTGTTTGGCA AGACACACAA GCTGAAGCCA GGAGTGAAGG AGCCTGTTGG TGTTAATAAA
    GCAGCACCTG CTCCACCCGT TGAGGATGTT AGCATGAGGA TCTATCTGTG TGAGGGGCTT
    CTGGGTAAAG AGCGCTCAAC TCTGTGGGAT CAGATGCAGT TCTGGGAGGA CGCCTTCCTG
    GATGCCGTAA TGCTGGAACG GGAAGGAATG GGAATGGATC AGGGACCACA AGAAATGATT
    GACAGGTATC TTTCTCTGGG AGATCATGAC CGCAAGAGAC TGGAGGATGA TGAGGACAGA
    CTGCTGGCCA CGCTTCTGCA CAATATGATT GCTTACATGC TTATGATGAA GGTCGGTAAA
    AACGACATCA GGAAGAAAGT ACGTCGTCTT ATGGGCAAAT CCCACATTGG CCTCACTCAC
    AGTCAGGAGA TCAATGAAGT CCTGGATCGC CTCGCACATT TAAGCGGTCG GGAGCTTTCT
    ATCAGGCCGA GTGGCAGCAG GCATATTAAA AAACAGACAT TTGTTGTGCA TGCTGGGACA
    GATACAACTG GTGACATATT CTTCATGGAG GTGTGTGATG ATTGCATCGT CTTGCGCAGT
    AATATTGGCA CAGTCTATGA GCGCTGGTGG TATGAGAAGC TCATAAATAT GACTTACTGT
    CCCAAAACTA AAGTGTTGTG TCTGTGGAGG AGAAACGGCC AGGAGACGCA GCTAAACAAG
    TTTTACACTA AGAAGTGTCG GGAATTGTAC TACTGTGTTA AAGACAGCAT GGAGCGTGCT
    GCTGCTCGAC AACAAAGCAT CAAACCAGGT CCAGAGCTTG GAGGCGAGTT TCCGGTTCAG
    GACATGAAGA CAGGGGAGGG TGGTCTCCTC CAAGTCACAC TGGAAGGCAT CAACCTCAAA
    TTTATGCACA GCCAGGTTTT CATAGAGCTG AATCACATTA AAAAGTGCAA TACAGTGAAA
    GGAGTCTTTG TCCTGGAGGA GTTTGTTCCT GAAACTAAAG AAGTGGTGAT CCATAAGTAC
    AAGACTCCTA TGGCACATCA GATCTGTTAC TCGGTGCTCT GCCTCTTCTC CTACATGGCG
    GCGGTCAAGG GGAAAGAGTC AGAGGGCAAA CCCAAGATGC TGTCCCCTCG TCCACTAGCG
    AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301560.1
    CDS435..5300
    Translation

    Target ORF information:

    RefSeq Version XM_009303285.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303285.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GTATTTGGGC TAAATTCTCT AATGGAGATT
    GTAACAGAGA TCGGCCCGGG GAGCGGAGAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGGAGCGGAT GGAGTAAAAC AACGTGTAGA AGGAGGAGAC
    ACTGAGGAGA AGAAATCACA GATCAGTGCA GACAGTGGCC TCAGTGTGAC ATCTGGCTCA
    CAGAAGAGTG ATACGGATTC CCTGGCGAGC TCTGAACCTC CTGCTCTGAC CAGAAGCACC
    AGCCAGGACT CAGAGGCCAG CACAGTGGTA AGTAACAGCT CAGGTGAGAC GCTCGGTGCA
    GACAGTGACC TGAGCAGCAC TGCTGGAGAT GGCCTGACAG GAAGACATGG TCAGCATCTC
    AACCTGTCCC GTGGCACACT GTCTGACAGT GAGATTGAGA CCAACCCTGC AACCAGCTCA
    GTGTTTGGCA AGACACACAA GCTGAAGCCA GGAGTGAAGG AGCCTGTTGG TGTTAATAAA
    GCAGCACCTG CTCCACCCGT TGAGGATGTT AGCATGAGGA TCTATCTGTG TGAGGGGCTT
    CTGGGTAAAG AGCGCTCAAC TCTGTGGGAT CAGATGCAGT TCTGGGAGGA CGCCTTCCTG
    GATGCCGTAA TGCTGGAACG GGAAGGAATG GGAATGGATC AGGGACCACA AGAAATGATT
    GACAGGTATC TTTCTCTGGG AGATCATGAC CGCAAGAGAC TGGAGGATGA TGAGGACAGA
    CTGCTGGCCA CGCTTCTGCA CAATATGATT GCTTACATGC TTATGATGAA GGTCGGTAAA
    AACGACATCA GGAAGAAAGT ACGTCGTCTT ATGGGCAAAT CCCACATTGG CCTCACTCAC
    AGTCAGGAGA TCAATGAAGT CCTGGATCGC CTCGCACATT TAAGCGGTCG GGAGCTTTCT
    ATCAGGCCGA GTGGCAGCAG GCATATTAAA AAACAGACAT TTGTTGTGCA TGCTGGGACA
    GATACAACTG GTGACATATT CTTCATGGAG GTGTGTGATG ATTGCATCGT CTTGCGCAGT
    AATATTGGCA CAGTCTATGA GCGCTGGTGG TATGAGAAGC TCATAAATAT GACTTACTGT
    CCCAAAACTA AAGTGTTGTG TCTGTGGAGG AGAAACGGCC AGGAGACGCA GCTAAACAAG
    TTTTACACTA AGAAGTGTCG GGAATTGTAC TACTGTGTTA AAGACAGCAT GGAGCGTGCT
    GCTGCTCGAC AACAAAGCAT CAAACCAGGT CCAGAGCTTG GAGGCGAGTT TCCGGTTCAG
    GACATGAAGA CAGGGGAGGG TGGTCTCCTC CAAGTCACAC TGGAAGGCAT CAACCTCAAA
    TTTATGCACA GCCAGGTTTT CATAGAGCTG AATCACATTA AAAAGTGCAA TACAGTGAAA
    GGAGTCTTTG TCCTGGAGGA GTTTGTTCCT GAAACTAAAG AAGTGGTGAT CCATAAGTAC
    AAGACTCCTA TGGCACATCA GATCTGTTAC TCGGTGCTCT GCCTCTTCTC CTACATGGCG
    GCGGTCAAGG GGAAAGAGTC AGAGGGCAAA CCCAAGATGC TGTCCCCTCG TCCACTAGCG
    AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa13173
    Clone ID Related Accession (Same CDS sequence) XM_009303285.2 , XM_021477902.1
    Accession Version XM_021477902.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4866bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X28
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GTATTTGGGC TAAATTCTCT AATGGAGATT
    GTAACAGAGA TCGGCCCGGG GAGCGGAGAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGGAGCGGAT GGAGTAAAAC AACGTGTAGA AGGAGGAGAC
    ACTGAGGAGA AGAAATCACA GATCAGTGCA GACAGTGGCC TCAGTGTGAC ATCTGGCTCA
    CAGAAGAGTG ATACGGATTC CCTGGCGAGC TCTGAACCTC CTGCTCTGAC CAGAAGCACC
    AGCCAGGACT CAGAGGCCAG CACAGTGGTA AGTAACAGCT CAGGTGAGAC GCTCGGTGCA
    GACAGTGACC TGAGCAGCAC TGCTGGAGAT GGCCTGACAG GAAGACATGG TCAGCATCTC
    AACCTGTCCC GTGGCACACT GTCTGACAGT GAGATTGAGA CCAACCCTGC AACCAGCTCA
    GTGTTTGGCA AGACACACAA GCTGAAGCCA GGAGTGAAGG AGCCTGTTGG TGTTAATAAA
    GCAGCACCTG CTCCACCCGT TGAGGATGTT AGCATGAGGA TCTATCTGTG TGAGGGGCTT
    CTGGGTAAAG AGCGCTCAAC TCTGTGGGAT CAGATGCAGT TCTGGGAGGA CGCCTTCCTG
    GATGCCGTAA TGCTGGAACG GGAAGGAATG GGAATGGATC AGGGACCACA AGAAATGATT
    GACAGGTATC TTTCTCTGGG AGATCATGAC CGCAAGAGAC TGGAGGATGA TGAGGACAGA
    CTGCTGGCCA CGCTTCTGCA CAATATGATT GCTTACATGC TTATGATGAA GGTCGGTAAA
    AACGACATCA GGAAGAAAGT ACGTCGTCTT ATGGGCAAAT CCCACATTGG CCTCACTCAC
    AGTCAGGAGA TCAATGAAGT CCTGGATCGC CTCGCACATT TAAGCGGTCG GGAGCTTTCT
    ATCAGGCCGA GTGGCAGCAG GCATATTAAA AAACAGACAT TTGTTGTGCA TGCTGGGACA
    GATACAACTG GTGACATATT CTTCATGGAG GTGTGTGATG ATTGCATCGT CTTGCGCAGT
    AATATTGGCA CAGTCTATGA GCGCTGGTGG TATGAGAAGC TCATAAATAT GACTTACTGT
    CCCAAAACTA AAGTGTTGTG TCTGTGGAGG AGAAACGGCC AGGAGACGCA GCTAAACAAG
    TTTTACACTA AGAAGTGTCG GGAATTGTAC TACTGTGTTA AAGACAGCAT GGAGCGTGCT
    GCTGCTCGAC AACAAAGCAT CAAACCAGGT CCAGAGCTTG GAGGCGAGTT TCCGGTTCAG
    GACATGAAGA CAGGGGAGGG TGGTCTCCTC CAAGTCACAC TGGAAGGCAT CAACCTCAAA
    TTTATGCACA GCCAGGTTTT CATAGAGCTG AATCACATTA AAAAGTGCAA TACAGTGAAA
    GGAGTCTTTG TCCTGGAGGA GTTTGTTCCT GAAACTAAAG AAGTGGTGAT CCATAAGTAC
    AAGACTCCTA TGGCACATCA GATCTGTTAC TCGGTGCTCT GCCTCTTCTC CTACATGGCG
    GCGGTCAAGG GGAAAGAGTC AGAGGGCAAA CCCAAGATGC TGTCCCCTCG TCCACTAGCG
    AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333577.1
    CDS1..4866
    Translation

    Target ORF information:

    RefSeq Version XM_021477902.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021477902.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GTATTTGGGC TAAATTCTCT AATGGAGATT
    GTAACAGAGA TCGGCCCGGG GAGCGGAGAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGGAGCGGAT GGAGTAAAAC AACGTGTAGA AGGAGGAGAC
    ACTGAGGAGA AGAAATCACA GATCAGTGCA GACAGTGGCC TCAGTGTGAC ATCTGGCTCA
    CAGAAGAGTG ATACGGATTC CCTGGCGAGC TCTGAACCTC CTGCTCTGAC CAGAAGCACC
    AGCCAGGACT CAGAGGCCAG CACAGTGGTA AGTAACAGCT CAGGTGAGAC GCTCGGTGCA
    GACAGTGACC TGAGCAGCAC TGCTGGAGAT GGCCTGACAG GAAGACATGG TCAGCATCTC
    AACCTGTCCC GTGGCACACT GTCTGACAGT GAGATTGAGA CCAACCCTGC AACCAGCTCA
    GTGTTTGGCA AGACACACAA GCTGAAGCCA GGAGTGAAGG AGCCTGTTGG TGTTAATAAA
    GCAGCACCTG CTCCACCCGT TGAGGATGTT AGCATGAGGA TCTATCTGTG TGAGGGGCTT
    CTGGGTAAAG AGCGCTCAAC TCTGTGGGAT CAGATGCAGT TCTGGGAGGA CGCCTTCCTG
    GATGCCGTAA TGCTGGAACG GGAAGGAATG GGAATGGATC AGGGACCACA AGAAATGATT
    GACAGGTATC TTTCTCTGGG AGATCATGAC CGCAAGAGAC TGGAGGATGA TGAGGACAGA
    CTGCTGGCCA CGCTTCTGCA CAATATGATT GCTTACATGC TTATGATGAA GGTCGGTAAA
    AACGACATCA GGAAGAAAGT ACGTCGTCTT ATGGGCAAAT CCCACATTGG CCTCACTCAC
    AGTCAGGAGA TCAATGAAGT CCTGGATCGC CTCGCACATT TAAGCGGTCG GGAGCTTTCT
    ATCAGGCCGA GTGGCAGCAG GCATATTAAA AAACAGACAT TTGTTGTGCA TGCTGGGACA
    GATACAACTG GTGACATATT CTTCATGGAG GTGTGTGATG ATTGCATCGT CTTGCGCAGT
    AATATTGGCA CAGTCTATGA GCGCTGGTGG TATGAGAAGC TCATAAATAT GACTTACTGT
    CCCAAAACTA AAGTGTTGTG TCTGTGGAGG AGAAACGGCC AGGAGACGCA GCTAAACAAG
    TTTTACACTA AGAAGTGTCG GGAATTGTAC TACTGTGTTA AAGACAGCAT GGAGCGTGCT
    GCTGCTCGAC AACAAAGCAT CAAACCAGGT CCAGAGCTTG GAGGCGAGTT TCCGGTTCAG
    GACATGAAGA CAGGGGAGGG TGGTCTCCTC CAAGTCACAC TGGAAGGCAT CAACCTCAAA
    TTTATGCACA GCCAGGTTTT CATAGAGCTG AATCACATTA AAAAGTGCAA TACAGTGAAA
    GGAGTCTTTG TCCTGGAGGA GTTTGTTCCT GAAACTAAAG AAGTGGTGAT CCATAAGTAC
    AAGACTCCTA TGGCACATCA GATCTGTTAC TCGGTGCTCT GCCTCTTCTC CTACATGGCG
    GCGGTCAAGG GGAAAGAGTC AGAGGGCAAA CCCAAGATGC TGTCCCCTCG TCCACTAGCG
    AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa13174
    Clone ID Related Accession (Same CDS sequence) XM_009303290.2 , XM_021477904.1
    Accession Version XM_009303290.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4806bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product MAP kinase-activating death domain protein isoform X27
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303290.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGGAGCGGAT GGAGTAAAAC AACGTGTAGA AGGAGGAGAC
    ACTGAGGAGA AGAAATCACA GATCAGTGCA GACAGTGGCC TCAGTGTGAC ATCTGGCTCA
    CAGAAGAGTG ATACGGATTC CCTGGCGAGC TCTGAACCTC CTGCTCTGAC CAGAAGCACC
    AGCCAGGACT CAGAGGCCAG CACAGTGGTA AGTAACAGCT CAGGTGAGAC GCTCGGTGCA
    GACAGTGACC TGAGCAGCAC TGCTGGAGAT GGCCTGACAG GAAGACATGG TCAGCATCTC
    AACCTGTCCC GTGGCACACT GTCTGACAGT GAGATTGAGA CCAACCCTGC AACCAGCTCA
    GTGTTTGGCA AGACACACAA GCTGAAGCCA GGAGTGAAGG AGCCTGTTGG TGTTAATAAA
    GCAGCACCTG CTCCACCCGT TGAGGATGTT AGCATGAGGA TCTATCTGTG TGAGGGGCTT
    CTGGGTAAAG AGCGCTCAAC TCTGTGGGAT CAGATGCAGT TCTGGGAGGA CGCCTTCCTG
    GATGCCGTAA TGCTGGAACG GGAAGGAATG GGAATGGATC AGGGACCACA AGAAATGATT
    GACAGGTATC TTTCTCTGGG AGATCATGAC CGCAAGAGAC TGGAGGATGA TGAGGACAGA
    CTGCTGGCCA CGCTTCTGCA CAATATGATT GCTTACATGC TTATGATGAA GGTCGGTAAA
    AACGACATCA GGAAGAAAGT ACGTCGTCTT ATGGGCAAAT CCCACATTGG CCTCACTCAC
    AGTCAGGAGA TCAATGAAGT CCTGGATCGC CTCGCACATT TAAGCGGTCG GGAGCTTTCT
    ATCAGGCCGA GTGGCAGCAG GCATATTAAA AAACAGACAT TTGTTGTGCA TGCTGGGACA
    GATACAACTG GTGACATATT CTTCATGGAG GTGTGTGATG ATTGCATCGT CTTGCGCAGT
    AATATTGGCA CAGTCTATGA GCGCTGGTGG TATGAGAAGC TCATAAATAT GACTTACTGT
    CCCAAAACTA AAGTGTTGTG TCTGTGGAGG AGAAACGGCC AGGAGACGCA GCTAAACAAG
    TTTTACACTA AGAAGTGTCG GGAATTGTAC TACTGTGTTA AAGACAGCAT GGAGCGTGCT
    GCTGCTCGAC AACAAAGCAT CAAACCAGGT CCAGAGCTTG GAGGCGAGTT TCCGGTTCAG
    GACATGAAGA CAGGGGAGGG TGGTCTCCTC CAAGTCACAC TGGAAGGCAT CAACCTCAAA
    TTTATGCACA GCCAGGTTTT CATAGAGCTG AATCACATTA AAAAGTGCAA TACAGTGAAA
    GGAGTCTTTG TCCTGGAGGA GTTTGTTCCT GAAACTAAAG AAGTGGTGAT CCATAAGTAC
    AAGACTCCTA TGGCACATCA GATCTGTTAC TCGGTGCTCT GCCTCTTCTC CTACATGGCG
    GCGGTCAAGG GGAAAGAGTC AGAGGGCAAA CCCAAGATGC TGTCCCCTCG TCCACTAGCG
    AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301565.1
    CDS434..5239
    Translation

    Target ORF information:

    RefSeq Version XM_009303290.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X27, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303290.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGGAGCGGAT GGAGTAAAAC AACGTGTAGA AGGAGGAGAC
    ACTGAGGAGA AGAAATCACA GATCAGTGCA GACAGTGGCC TCAGTGTGAC ATCTGGCTCA
    CAGAAGAGTG ATACGGATTC CCTGGCGAGC TCTGAACCTC CTGCTCTGAC CAGAAGCACC
    AGCCAGGACT CAGAGGCCAG CACAGTGGTA AGTAACAGCT CAGGTGAGAC GCTCGGTGCA
    GACAGTGACC TGAGCAGCAC TGCTGGAGAT GGCCTGACAG GAAGACATGG TCAGCATCTC
    AACCTGTCCC GTGGCACACT GTCTGACAGT GAGATTGAGA CCAACCCTGC AACCAGCTCA
    GTGTTTGGCA AGACACACAA GCTGAAGCCA GGAGTGAAGG AGCCTGTTGG TGTTAATAAA
    GCAGCACCTG CTCCACCCGT TGAGGATGTT AGCATGAGGA TCTATCTGTG TGAGGGGCTT
    CTGGGTAAAG AGCGCTCAAC TCTGTGGGAT CAGATGCAGT TCTGGGAGGA CGCCTTCCTG
    GATGCCGTAA TGCTGGAACG GGAAGGAATG GGAATGGATC AGGGACCACA AGAAATGATT
    GACAGGTATC TTTCTCTGGG AGATCATGAC CGCAAGAGAC TGGAGGATGA TGAGGACAGA
    CTGCTGGCCA CGCTTCTGCA CAATATGATT GCTTACATGC TTATGATGAA GGTCGGTAAA
    AACGACATCA GGAAGAAAGT ACGTCGTCTT ATGGGCAAAT CCCACATTGG CCTCACTCAC
    AGTCAGGAGA TCAATGAAGT CCTGGATCGC CTCGCACATT TAAGCGGTCG GGAGCTTTCT
    ATCAGGCCGA GTGGCAGCAG GCATATTAAA AAACAGACAT TTGTTGTGCA TGCTGGGACA
    GATACAACTG GTGACATATT CTTCATGGAG GTGTGTGATG ATTGCATCGT CTTGCGCAGT
    AATATTGGCA CAGTCTATGA GCGCTGGTGG TATGAGAAGC TCATAAATAT GACTTACTGT
    CCCAAAACTA AAGTGTTGTG TCTGTGGAGG AGAAACGGCC AGGAGACGCA GCTAAACAAG
    TTTTACACTA AGAAGTGTCG GGAATTGTAC TACTGTGTTA AAGACAGCAT GGAGCGTGCT
    GCTGCTCGAC AACAAAGCAT CAAACCAGGT CCAGAGCTTG GAGGCGAGTT TCCGGTTCAG
    GACATGAAGA CAGGGGAGGG TGGTCTCCTC CAAGTCACAC TGGAAGGCAT CAACCTCAAA
    TTTATGCACA GCCAGGTTTT CATAGAGCTG AATCACATTA AAAAGTGCAA TACAGTGAAA
    GGAGTCTTTG TCCTGGAGGA GTTTGTTCCT GAAACTAAAG AAGTGGTGAT CCATAAGTAC
    AAGACTCCTA TGGCACATCA GATCTGTTAC TCGGTGCTCT GCCTCTTCTC CTACATGGCG
    GCGGTCAAGG GGAAAGAGTC AGAGGGCAAA CCCAAGATGC TGTCCCCTCG TCCACTAGCG
    AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa13174
    Clone ID Related Accession (Same CDS sequence) XM_009303290.2 , XM_021477904.1
    Accession Version XM_021477904.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4806bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X30
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGGAGCGGAT GGAGTAAAAC AACGTGTAGA AGGAGGAGAC
    ACTGAGGAGA AGAAATCACA GATCAGTGCA GACAGTGGCC TCAGTGTGAC ATCTGGCTCA
    CAGAAGAGTG ATACGGATTC CCTGGCGAGC TCTGAACCTC CTGCTCTGAC CAGAAGCACC
    AGCCAGGACT CAGAGGCCAG CACAGTGGTA AGTAACAGCT CAGGTGAGAC GCTCGGTGCA
    GACAGTGACC TGAGCAGCAC TGCTGGAGAT GGCCTGACAG GAAGACATGG TCAGCATCTC
    AACCTGTCCC GTGGCACACT GTCTGACAGT GAGATTGAGA CCAACCCTGC AACCAGCTCA
    GTGTTTGGCA AGACACACAA GCTGAAGCCA GGAGTGAAGG AGCCTGTTGG TGTTAATAAA
    GCAGCACCTG CTCCACCCGT TGAGGATGTT AGCATGAGGA TCTATCTGTG TGAGGGGCTT
    CTGGGTAAAG AGCGCTCAAC TCTGTGGGAT CAGATGCAGT TCTGGGAGGA CGCCTTCCTG
    GATGCCGTAA TGCTGGAACG GGAAGGAATG GGAATGGATC AGGGACCACA AGAAATGATT
    GACAGGTATC TTTCTCTGGG AGATCATGAC CGCAAGAGAC TGGAGGATGA TGAGGACAGA
    CTGCTGGCCA CGCTTCTGCA CAATATGATT GCTTACATGC TTATGATGAA GGTCGGTAAA
    AACGACATCA GGAAGAAAGT ACGTCGTCTT ATGGGCAAAT CCCACATTGG CCTCACTCAC
    AGTCAGGAGA TCAATGAAGT CCTGGATCGC CTCGCACATT TAAGCGGTCG GGAGCTTTCT
    ATCAGGCCGA GTGGCAGCAG GCATATTAAA AAACAGACAT TTGTTGTGCA TGCTGGGACA
    GATACAACTG GTGACATATT CTTCATGGAG GTGTGTGATG ATTGCATCGT CTTGCGCAGT
    AATATTGGCA CAGTCTATGA GCGCTGGTGG TATGAGAAGC TCATAAATAT GACTTACTGT
    CCCAAAACTA AAGTGTTGTG TCTGTGGAGG AGAAACGGCC AGGAGACGCA GCTAAACAAG
    TTTTACACTA AGAAGTGTCG GGAATTGTAC TACTGTGTTA AAGACAGCAT GGAGCGTGCT
    GCTGCTCGAC AACAAAGCAT CAAACCAGGT CCAGAGCTTG GAGGCGAGTT TCCGGTTCAG
    GACATGAAGA CAGGGGAGGG TGGTCTCCTC CAAGTCACAC TGGAAGGCAT CAACCTCAAA
    TTTATGCACA GCCAGGTTTT CATAGAGCTG AATCACATTA AAAAGTGCAA TACAGTGAAA
    GGAGTCTTTG TCCTGGAGGA GTTTGTTCCT GAAACTAAAG AAGTGGTGAT CCATAAGTAC
    AAGACTCCTA TGGCACATCA GATCTGTTAC TCGGTGCTCT GCCTCTTCTC CTACATGGCG
    GCGGTCAAGG GGAAAGAGTC AGAGGGCAAA CCCAAGATGC TGTCCCCTCG TCCACTAGCG
    AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333579.1
    CDS1..4806
    Translation

    Target ORF information:

    RefSeq Version XM_021477904.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021477904.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGGAGCGGAT GGAGTAAAAC AACGTGTAGA AGGAGGAGAC
    ACTGAGGAGA AGAAATCACA GATCAGTGCA GACAGTGGCC TCAGTGTGAC ATCTGGCTCA
    CAGAAGAGTG ATACGGATTC CCTGGCGAGC TCTGAACCTC CTGCTCTGAC CAGAAGCACC
    AGCCAGGACT CAGAGGCCAG CACAGTGGTA AGTAACAGCT CAGGTGAGAC GCTCGGTGCA
    GACAGTGACC TGAGCAGCAC TGCTGGAGAT GGCCTGACAG GAAGACATGG TCAGCATCTC
    AACCTGTCCC GTGGCACACT GTCTGACAGT GAGATTGAGA CCAACCCTGC AACCAGCTCA
    GTGTTTGGCA AGACACACAA GCTGAAGCCA GGAGTGAAGG AGCCTGTTGG TGTTAATAAA
    GCAGCACCTG CTCCACCCGT TGAGGATGTT AGCATGAGGA TCTATCTGTG TGAGGGGCTT
    CTGGGTAAAG AGCGCTCAAC TCTGTGGGAT CAGATGCAGT TCTGGGAGGA CGCCTTCCTG
    GATGCCGTAA TGCTGGAACG GGAAGGAATG GGAATGGATC AGGGACCACA AGAAATGATT
    GACAGGTATC TTTCTCTGGG AGATCATGAC CGCAAGAGAC TGGAGGATGA TGAGGACAGA
    CTGCTGGCCA CGCTTCTGCA CAATATGATT GCTTACATGC TTATGATGAA GGTCGGTAAA
    AACGACATCA GGAAGAAAGT ACGTCGTCTT ATGGGCAAAT CCCACATTGG CCTCACTCAC
    AGTCAGGAGA TCAATGAAGT CCTGGATCGC CTCGCACATT TAAGCGGTCG GGAGCTTTCT
    ATCAGGCCGA GTGGCAGCAG GCATATTAAA AAACAGACAT TTGTTGTGCA TGCTGGGACA
    GATACAACTG GTGACATATT CTTCATGGAG GTGTGTGATG ATTGCATCGT CTTGCGCAGT
    AATATTGGCA CAGTCTATGA GCGCTGGTGG TATGAGAAGC TCATAAATAT GACTTACTGT
    CCCAAAACTA AAGTGTTGTG TCTGTGGAGG AGAAACGGCC AGGAGACGCA GCTAAACAAG
    TTTTACACTA AGAAGTGTCG GGAATTGTAC TACTGTGTTA AAGACAGCAT GGAGCGTGCT
    GCTGCTCGAC AACAAAGCAT CAAACCAGGT CCAGAGCTTG GAGGCGAGTT TCCGGTTCAG
    GACATGAAGA CAGGGGAGGG TGGTCTCCTC CAAGTCACAC TGGAAGGCAT CAACCTCAAA
    TTTATGCACA GCCAGGTTTT CATAGAGCTG AATCACATTA AAAAGTGCAA TACAGTGAAA
    GGAGTCTTTG TCCTGGAGGA GTTTGTTCCT GAAACTAAAG AAGTGGTGAT CCATAAGTAC
    AAGACTCCTA TGGCACATCA GATCTGTTAC TCGGTGCTCT GCCTCTTCTC CTACATGGCG
    GCGGTCAAGG GGAAAGAGTC AGAGGGCAAA CCCAAGATGC TGTCCCCTCG TCCACTAGCG
    AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa13175
    Clone ID Related Accession (Same CDS sequence) XM_021477905.1 , XM_009303291.2
    Accession Version XM_021477905.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4803bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X31
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGGAGCGGAT GGAGTAAAAC AACGTGTAGA AGGAGGAGAC
    ACTGAGGAGA AGAAATCACA GATCAGTGCA GACAGTGGCC TCAGTGTGAC ATCTGGCTCA
    CAGAAGAGTG ATACGGATTC CCTGGCGAGC TCTGAACCTC CTGCTCTGAC CAGAAGCACC
    AGCCAGGACT CAGAGGCCAG CACAGTAAGT AACAGCTCAG GTGAGACGCT CGGTGCAGAC
    AGTGACCTGA GCAGCACTGC TGGAGATGGC CTGACAGGAA GACATGGTCA GCATCTCAAC
    CTGTCCCGTG GCACACTGTC TGACAGTGAG ATTGAGACCA ACCCTGCAAC CAGCTCAGTG
    TTTGGCAAGA CACACAAGCT GAAGCCAGGA GTGAAGGAGC CTGTTGGTGT TAATAAAGCA
    GCACCTGCTC CACCCGTTGA GGATGTTAGC ATGAGGATCT ATCTGTGTGA GGGGCTTCTG
    GGTAAAGAGC GCTCAACTCT GTGGGATCAG ATGCAGTTCT GGGAGGACGC CTTCCTGGAT
    GCCGTAATGC TGGAACGGGA AGGAATGGGA ATGGATCAGG GACCACAAGA AATGATTGAC
    AGGTATCTTT CTCTGGGAGA TCATGACCGC AAGAGACTGG AGGATGATGA GGACAGACTG
    CTGGCCACGC TTCTGCACAA TATGATTGCT TACATGCTTA TGATGAAGGT CGGTAAAAAC
    GACATCAGGA AGAAAGTACG TCGTCTTATG GGCAAATCCC ACATTGGCCT CACTCACAGT
    CAGGAGATCA ATGAAGTCCT GGATCGCCTC GCACATTTAA GCGGTCGGGA GCTTTCTATC
    AGGCCGAGTG GCAGCAGGCA TATTAAAAAA CAGACATTTG TTGTGCATGC TGGGACAGAT
    ACAACTGGTG ACATATTCTT CATGGAGGTG TGTGATGATT GCATCGTCTT GCGCAGTAAT
    ATTGGCACAG TCTATGAGCG CTGGTGGTAT GAGAAGCTCA TAAATATGAC TTACTGTCCC
    AAAACTAAAG TGTTGTGTCT GTGGAGGAGA AACGGCCAGG AGACGCAGCT AAACAAGTTT
    TACACTAAGA AGTGTCGGGA ATTGTACTAC TGTGTTAAAG ACAGCATGGA GCGTGCTGCT
    GCTCGACAAC AAAGCATCAA ACCAGGTCCA GAGCTTGGAG GCGAGTTTCC GGTTCAGGAC
    ATGAAGACAG GGGAGGGTGG TCTCCTCCAA GTCACACTGG AAGGCATCAA CCTCAAATTT
    ATGCACAGCC AGGTTTTCAT AGAGCTGAAT CACATTAAAA AGTGCAATAC AGTGAAAGGA
    GTCTTTGTCC TGGAGGAGTT TGTTCCTGAA ACTAAAGAAG TGGTGATCCA TAAGTACAAG
    ACTCCTATGG CACATCAGAT CTGTTACTCG GTGCTCTGCC TCTTCTCCTA CATGGCGGCG
    GTCAAGGGGA AAGAGTCAGA GGGCAAACCC AAGATGCTGT CCCCTCGTCC ACTAGCGAGC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333580.1
    CDS1..4803
    Translation

    Target ORF information:

    RefSeq Version XM_021477905.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021477905.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGGAGCGGAT GGAGTAAAAC AACGTGTAGA AGGAGGAGAC
    ACTGAGGAGA AGAAATCACA GATCAGTGCA GACAGTGGCC TCAGTGTGAC ATCTGGCTCA
    CAGAAGAGTG ATACGGATTC CCTGGCGAGC TCTGAACCTC CTGCTCTGAC CAGAAGCACC
    AGCCAGGACT CAGAGGCCAG CACAGTAAGT AACAGCTCAG GTGAGACGCT CGGTGCAGAC
    AGTGACCTGA GCAGCACTGC TGGAGATGGC CTGACAGGAA GACATGGTCA GCATCTCAAC
    CTGTCCCGTG GCACACTGTC TGACAGTGAG ATTGAGACCA ACCCTGCAAC CAGCTCAGTG
    TTTGGCAAGA CACACAAGCT GAAGCCAGGA GTGAAGGAGC CTGTTGGTGT TAATAAAGCA
    GCACCTGCTC CACCCGTTGA GGATGTTAGC ATGAGGATCT ATCTGTGTGA GGGGCTTCTG
    GGTAAAGAGC GCTCAACTCT GTGGGATCAG ATGCAGTTCT GGGAGGACGC CTTCCTGGAT
    GCCGTAATGC TGGAACGGGA AGGAATGGGA ATGGATCAGG GACCACAAGA AATGATTGAC
    AGGTATCTTT CTCTGGGAGA TCATGACCGC AAGAGACTGG AGGATGATGA GGACAGACTG
    CTGGCCACGC TTCTGCACAA TATGATTGCT TACATGCTTA TGATGAAGGT CGGTAAAAAC
    GACATCAGGA AGAAAGTACG TCGTCTTATG GGCAAATCCC ACATTGGCCT CACTCACAGT
    CAGGAGATCA ATGAAGTCCT GGATCGCCTC GCACATTTAA GCGGTCGGGA GCTTTCTATC
    AGGCCGAGTG GCAGCAGGCA TATTAAAAAA CAGACATTTG TTGTGCATGC TGGGACAGAT
    ACAACTGGTG ACATATTCTT CATGGAGGTG TGTGATGATT GCATCGTCTT GCGCAGTAAT
    ATTGGCACAG TCTATGAGCG CTGGTGGTAT GAGAAGCTCA TAAATATGAC TTACTGTCCC
    AAAACTAAAG TGTTGTGTCT GTGGAGGAGA AACGGCCAGG AGACGCAGCT AAACAAGTTT
    TACACTAAGA AGTGTCGGGA ATTGTACTAC TGTGTTAAAG ACAGCATGGA GCGTGCTGCT
    GCTCGACAAC AAAGCATCAA ACCAGGTCCA GAGCTTGGAG GCGAGTTTCC GGTTCAGGAC
    ATGAAGACAG GGGAGGGTGG TCTCCTCCAA GTCACACTGG AAGGCATCAA CCTCAAATTT
    ATGCACAGCC AGGTTTTCAT AGAGCTGAAT CACATTAAAA AGTGCAATAC AGTGAAAGGA
    GTCTTTGTCC TGGAGGAGTT TGTTCCTGAA ACTAAAGAAG TGGTGATCCA TAAGTACAAG
    ACTCCTATGG CACATCAGAT CTGTTACTCG GTGCTCTGCC TCTTCTCCTA CATGGCGGCG
    GTCAAGGGGA AAGAGTCAGA GGGCAAACCC AAGATGCTGT CCCCTCGTCC ACTAGCGAGC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa13175
    Clone ID Related Accession (Same CDS sequence) XM_021477905.1 , XM_009303291.2
    Accession Version XM_009303291.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4803bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product MAP kinase-activating death domain protein isoform X28
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303291.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGGAGCGGAT GGAGTAAAAC AACGTGTAGA AGGAGGAGAC
    ACTGAGGAGA AGAAATCACA GATCAGTGCA GACAGTGGCC TCAGTGTGAC ATCTGGCTCA
    CAGAAGAGTG ATACGGATTC CCTGGCGAGC TCTGAACCTC CTGCTCTGAC CAGAAGCACC
    AGCCAGGACT CAGAGGCCAG CACAGTAAGT AACAGCTCAG GTGAGACGCT CGGTGCAGAC
    AGTGACCTGA GCAGCACTGC TGGAGATGGC CTGACAGGAA GACATGGTCA GCATCTCAAC
    CTGTCCCGTG GCACACTGTC TGACAGTGAG ATTGAGACCA ACCCTGCAAC CAGCTCAGTG
    TTTGGCAAGA CACACAAGCT GAAGCCAGGA GTGAAGGAGC CTGTTGGTGT TAATAAAGCA
    GCACCTGCTC CACCCGTTGA GGATGTTAGC ATGAGGATCT ATCTGTGTGA GGGGCTTCTG
    GGTAAAGAGC GCTCAACTCT GTGGGATCAG ATGCAGTTCT GGGAGGACGC CTTCCTGGAT
    GCCGTAATGC TGGAACGGGA AGGAATGGGA ATGGATCAGG GACCACAAGA AATGATTGAC
    AGGTATCTTT CTCTGGGAGA TCATGACCGC AAGAGACTGG AGGATGATGA GGACAGACTG
    CTGGCCACGC TTCTGCACAA TATGATTGCT TACATGCTTA TGATGAAGGT CGGTAAAAAC
    GACATCAGGA AGAAAGTACG TCGTCTTATG GGCAAATCCC ACATTGGCCT CACTCACAGT
    CAGGAGATCA ATGAAGTCCT GGATCGCCTC GCACATTTAA GCGGTCGGGA GCTTTCTATC
    AGGCCGAGTG GCAGCAGGCA TATTAAAAAA CAGACATTTG TTGTGCATGC TGGGACAGAT
    ACAACTGGTG ACATATTCTT CATGGAGGTG TGTGATGATT GCATCGTCTT GCGCAGTAAT
    ATTGGCACAG TCTATGAGCG CTGGTGGTAT GAGAAGCTCA TAAATATGAC TTACTGTCCC
    AAAACTAAAG TGTTGTGTCT GTGGAGGAGA AACGGCCAGG AGACGCAGCT AAACAAGTTT
    TACACTAAGA AGTGTCGGGA ATTGTACTAC TGTGTTAAAG ACAGCATGGA GCGTGCTGCT
    GCTCGACAAC AAAGCATCAA ACCAGGTCCA GAGCTTGGAG GCGAGTTTCC GGTTCAGGAC
    ATGAAGACAG GGGAGGGTGG TCTCCTCCAA GTCACACTGG AAGGCATCAA CCTCAAATTT
    ATGCACAGCC AGGTTTTCAT AGAGCTGAAT CACATTAAAA AGTGCAATAC AGTGAAAGGA
    GTCTTTGTCC TGGAGGAGTT TGTTCCTGAA ACTAAAGAAG TGGTGATCCA TAAGTACAAG
    ACTCCTATGG CACATCAGAT CTGTTACTCG GTGCTCTGCC TCTTCTCCTA CATGGCGGCG
    GTCAAGGGGA AAGAGTCAGA GGGCAAACCC AAGATGCTGT CCCCTCGTCC ACTAGCGAGC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301566.1
    CDS435..5237
    Translation

    Target ORF information:

    RefSeq Version XM_009303291.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X28, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303291.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGGAGCGGAT GGAGTAAAAC AACGTGTAGA AGGAGGAGAC
    ACTGAGGAGA AGAAATCACA GATCAGTGCA GACAGTGGCC TCAGTGTGAC ATCTGGCTCA
    CAGAAGAGTG ATACGGATTC CCTGGCGAGC TCTGAACCTC CTGCTCTGAC CAGAAGCACC
    AGCCAGGACT CAGAGGCCAG CACAGTAAGT AACAGCTCAG GTGAGACGCT CGGTGCAGAC
    AGTGACCTGA GCAGCACTGC TGGAGATGGC CTGACAGGAA GACATGGTCA GCATCTCAAC
    CTGTCCCGTG GCACACTGTC TGACAGTGAG ATTGAGACCA ACCCTGCAAC CAGCTCAGTG
    TTTGGCAAGA CACACAAGCT GAAGCCAGGA GTGAAGGAGC CTGTTGGTGT TAATAAAGCA
    GCACCTGCTC CACCCGTTGA GGATGTTAGC ATGAGGATCT ATCTGTGTGA GGGGCTTCTG
    GGTAAAGAGC GCTCAACTCT GTGGGATCAG ATGCAGTTCT GGGAGGACGC CTTCCTGGAT
    GCCGTAATGC TGGAACGGGA AGGAATGGGA ATGGATCAGG GACCACAAGA AATGATTGAC
    AGGTATCTTT CTCTGGGAGA TCATGACCGC AAGAGACTGG AGGATGATGA GGACAGACTG
    CTGGCCACGC TTCTGCACAA TATGATTGCT TACATGCTTA TGATGAAGGT CGGTAAAAAC
    GACATCAGGA AGAAAGTACG TCGTCTTATG GGCAAATCCC ACATTGGCCT CACTCACAGT
    CAGGAGATCA ATGAAGTCCT GGATCGCCTC GCACATTTAA GCGGTCGGGA GCTTTCTATC
    AGGCCGAGTG GCAGCAGGCA TATTAAAAAA CAGACATTTG TTGTGCATGC TGGGACAGAT
    ACAACTGGTG ACATATTCTT CATGGAGGTG TGTGATGATT GCATCGTCTT GCGCAGTAAT
    ATTGGCACAG TCTATGAGCG CTGGTGGTAT GAGAAGCTCA TAAATATGAC TTACTGTCCC
    AAAACTAAAG TGTTGTGTCT GTGGAGGAGA AACGGCCAGG AGACGCAGCT AAACAAGTTT
    TACACTAAGA AGTGTCGGGA ATTGTACTAC TGTGTTAAAG ACAGCATGGA GCGTGCTGCT
    GCTCGACAAC AAAGCATCAA ACCAGGTCCA GAGCTTGGAG GCGAGTTTCC GGTTCAGGAC
    ATGAAGACAG GGGAGGGTGG TCTCCTCCAA GTCACACTGG AAGGCATCAA CCTCAAATTT
    ATGCACAGCC AGGTTTTCAT AGAGCTGAAT CACATTAAAA AGTGCAATAC AGTGAAAGGA
    GTCTTTGTCC TGGAGGAGTT TGTTCCTGAA ACTAAAGAAG TGGTGATCCA TAAGTACAAG
    ACTCCTATGG CACATCAGAT CTGTTACTCG GTGCTCTGCC TCTTCTCCTA CATGGCGGCG
    GTCAAGGGGA AAGAGTCAGA GGGCAAACCC AAGATGCTGT CCCCTCGTCC ACTAGCGAGC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa13176
    Clone ID Related Accession (Same CDS sequence) XM_009303298.2 , XM_009303298.3
    Accession Version XM_009303298.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4599bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product MAP kinase-activating death domain protein isoform X35
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303298.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGGAGCGGAT GGAGTAAAAC AACGTGTAGA AGGAGGAGAC
    ACTGAGGAGA AGAAATCACA GATCAGTGCA GACAGTGGCC TCAGTGTGAC ATCTGGCTCA
    CAGAAGAGTG ATACGGATTC CCTGGCGAGC TCTGAACCTC CTGCTCTGAC CAGAAGCACC
    AGCCAGGACT CAGAGGCCAG CACAGTGGTA AGTAACAGCT CAGGTGAGAC GCTCGGTGCA
    GACAGTGACC TGAGCAGCAC TGCTGGAGAT GGCCTGACAG GAAGACATGG TCAGCATCTC
    AACCTGTCCC GTGGCACACT GTCTGACAGT GAGATTGAGA CCAACCCTGC AACCAGCTCA
    GTGTTTGGCA AGACACACAA GCTGAAGCCA GGAGTGAAGG AGCCTGTTGG TGTTAATAAA
    GCAGCACCTG CTCCACCCGT TGAGGATGTT AGCATGAGGA TCTATCTGTG TGAGGGGCTT
    CTGGGTAAAG AGCGCTCAAC TCTGTGGGAT CAGATGCAGT TCTGGGAGGA CGCCTTCCTG
    GATGCCGTAA TGCTGGAACG GGAAGGAATG GGAATGGATC AGGGACCACA AGAAATGATT
    GACAGGTATC TTTCTCTGGG AGATCATGAC CGCAAGAGAC TGGAGGATGA TGAGGACAGA
    CTGCTGGCCA CGCTTCTGCA CAATATGATT GCTTACATGC TTATGATGAA GGTCGGTAAA
    AACGACATCA GGAAGAAAGT ACGTCGTCTT ATGGGCAAAT CCCACATTGG CCTCACTCAC
    AGTCAGGAGA TCAATGAAGT CCTGGATCGC CTCGCACATT TAAGCGGTCG GGAGCTTTCT
    ATCAGGCCGA GTGGCAGCAG GCATATTAAA AAACAGACAT TTGTTGTGCA TGCTGGGACA
    GATACAACTG GTGACATATT CTTCATGGAG GTGTGTGATG ATTGCATCGT CTTGCGCAGT
    AATATTGGCA CAGTCTATGA GCGCTGGTGG TATGAGAAGC TCATAAATAT GACTTACTGT
    CCCAAAACTA AAGTGTTGTG TCTGTGGAGG AGAAACGGCC AGGAGACGCA GCTAAACAAG
    TTTTACACTA AGAAGTGTCG GGAATTGTAC TACTGTGTTA AAGACAGCAT GGAGCGTGCT
    GCTGCTCGAC AACAAAGCAT CAAACCAGGT CCAGAGCTTG GAGGCGAGTT TCCGGTTCAG
    GACATGAAGA CAGGGGAGGG TGGTCTCCTC CAAGTCACAC TGGAAGGCAT CAACCTCAAA
    TTTATGCACA GCCAGTTCCT GAAACTAAAG AAGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301573.1
    CDS435..5033
    Translation

    Target ORF information:

    RefSeq Version XM_009303298.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X35, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303298.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGGAGCGGAT GGAGTAAAAC AACGTGTAGA AGGAGGAGAC
    ACTGAGGAGA AGAAATCACA GATCAGTGCA GACAGTGGCC TCAGTGTGAC ATCTGGCTCA
    CAGAAGAGTG ATACGGATTC CCTGGCGAGC TCTGAACCTC CTGCTCTGAC CAGAAGCACC
    AGCCAGGACT CAGAGGCCAG CACAGTGGTA AGTAACAGCT CAGGTGAGAC GCTCGGTGCA
    GACAGTGACC TGAGCAGCAC TGCTGGAGAT GGCCTGACAG GAAGACATGG TCAGCATCTC
    AACCTGTCCC GTGGCACACT GTCTGACAGT GAGATTGAGA CCAACCCTGC AACCAGCTCA
    GTGTTTGGCA AGACACACAA GCTGAAGCCA GGAGTGAAGG AGCCTGTTGG TGTTAATAAA
    GCAGCACCTG CTCCACCCGT TGAGGATGTT AGCATGAGGA TCTATCTGTG TGAGGGGCTT
    CTGGGTAAAG AGCGCTCAAC TCTGTGGGAT CAGATGCAGT TCTGGGAGGA CGCCTTCCTG
    GATGCCGTAA TGCTGGAACG GGAAGGAATG GGAATGGATC AGGGACCACA AGAAATGATT
    GACAGGTATC TTTCTCTGGG AGATCATGAC CGCAAGAGAC TGGAGGATGA TGAGGACAGA
    CTGCTGGCCA CGCTTCTGCA CAATATGATT GCTTACATGC TTATGATGAA GGTCGGTAAA
    AACGACATCA GGAAGAAAGT ACGTCGTCTT ATGGGCAAAT CCCACATTGG CCTCACTCAC
    AGTCAGGAGA TCAATGAAGT CCTGGATCGC CTCGCACATT TAAGCGGTCG GGAGCTTTCT
    ATCAGGCCGA GTGGCAGCAG GCATATTAAA AAACAGACAT TTGTTGTGCA TGCTGGGACA
    GATACAACTG GTGACATATT CTTCATGGAG GTGTGTGATG ATTGCATCGT CTTGCGCAGT
    AATATTGGCA CAGTCTATGA GCGCTGGTGG TATGAGAAGC TCATAAATAT GACTTACTGT
    CCCAAAACTA AAGTGTTGTG TCTGTGGAGG AGAAACGGCC AGGAGACGCA GCTAAACAAG
    TTTTACACTA AGAAGTGTCG GGAATTGTAC TACTGTGTTA AAGACAGCAT GGAGCGTGCT
    GCTGCTCGAC AACAAAGCAT CAAACCAGGT CCAGAGCTTG GAGGCGAGTT TCCGGTTCAG
    GACATGAAGA CAGGGGAGGG TGGTCTCCTC CAAGTCACAC TGGAAGGCAT CAACCTCAAA
    TTTATGCACA GCCAGTTCCT GAAACTAAAG AAGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa13176
    Clone ID Related Accession (Same CDS sequence) XM_009303298.2 , XM_009303298.3
    Accession Version XM_009303298.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4599bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X38
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303298.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGGAGCGGAT GGAGTAAAAC AACGTGTAGA AGGAGGAGAC
    ACTGAGGAGA AGAAATCACA GATCAGTGCA GACAGTGGCC TCAGTGTGAC ATCTGGCTCA
    CAGAAGAGTG ATACGGATTC CCTGGCGAGC TCTGAACCTC CTGCTCTGAC CAGAAGCACC
    AGCCAGGACT CAGAGGCCAG CACAGTGGTA AGTAACAGCT CAGGTGAGAC GCTCGGTGCA
    GACAGTGACC TGAGCAGCAC TGCTGGAGAT GGCCTGACAG GAAGACATGG TCAGCATCTC
    AACCTGTCCC GTGGCACACT GTCTGACAGT GAGATTGAGA CCAACCCTGC AACCAGCTCA
    GTGTTTGGCA AGACACACAA GCTGAAGCCA GGAGTGAAGG AGCCTGTTGG TGTTAATAAA
    GCAGCACCTG CTCCACCCGT TGAGGATGTT AGCATGAGGA TCTATCTGTG TGAGGGGCTT
    CTGGGTAAAG AGCGCTCAAC TCTGTGGGAT CAGATGCAGT TCTGGGAGGA CGCCTTCCTG
    GATGCCGTAA TGCTGGAACG GGAAGGAATG GGAATGGATC AGGGACCACA AGAAATGATT
    GACAGGTATC TTTCTCTGGG AGATCATGAC CGCAAGAGAC TGGAGGATGA TGAGGACAGA
    CTGCTGGCCA CGCTTCTGCA CAATATGATT GCTTACATGC TTATGATGAA GGTCGGTAAA
    AACGACATCA GGAAGAAAGT ACGTCGTCTT ATGGGCAAAT CCCACATTGG CCTCACTCAC
    AGTCAGGAGA TCAATGAAGT CCTGGATCGC CTCGCACATT TAAGCGGTCG GGAGCTTTCT
    ATCAGGCCGA GTGGCAGCAG GCATATTAAA AAACAGACAT TTGTTGTGCA TGCTGGGACA
    GATACAACTG GTGACATATT CTTCATGGAG GTGTGTGATG ATTGCATCGT CTTGCGCAGT
    AATATTGGCA CAGTCTATGA GCGCTGGTGG TATGAGAAGC TCATAAATAT GACTTACTGT
    CCCAAAACTA AAGTGTTGTG TCTGTGGAGG AGAAACGGCC AGGAGACGCA GCTAAACAAG
    TTTTACACTA AGAAGTGTCG GGAATTGTAC TACTGTGTTA AAGACAGCAT GGAGCGTGCT
    GCTGCTCGAC AACAAAGCAT CAAACCAGGT CCAGAGCTTG GAGGCGAGTT TCCGGTTCAG
    GACATGAAGA CAGGGGAGGG TGGTCTCCTC CAAGTCACAC TGGAAGGCAT CAACCTCAAA
    TTTATGCACA GCCAGTTCCT GAAACTAAAG AAGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301573.1
    CDS432..5030
    Translation

    Target ORF information:

    RefSeq Version XM_009303298.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X39, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303298.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGGAGCGGAT GGAGTAAAAC AACGTGTAGA AGGAGGAGAC
    ACTGAGGAGA AGAAATCACA GATCAGTGCA GACAGTGGCC TCAGTGTGAC ATCTGGCTCA
    CAGAAGAGTG ATACGGATTC CCTGGCGAGC TCTGAACCTC CTGCTCTGAC CAGAAGCACC
    AGCCAGGACT CAGAGGCCAG CACAGTGGTA AGTAACAGCT CAGGTGAGAC GCTCGGTGCA
    GACAGTGACC TGAGCAGCAC TGCTGGAGAT GGCCTGACAG GAAGACATGG TCAGCATCTC
    AACCTGTCCC GTGGCACACT GTCTGACAGT GAGATTGAGA CCAACCCTGC AACCAGCTCA
    GTGTTTGGCA AGACACACAA GCTGAAGCCA GGAGTGAAGG AGCCTGTTGG TGTTAATAAA
    GCAGCACCTG CTCCACCCGT TGAGGATGTT AGCATGAGGA TCTATCTGTG TGAGGGGCTT
    CTGGGTAAAG AGCGCTCAAC TCTGTGGGAT CAGATGCAGT TCTGGGAGGA CGCCTTCCTG
    GATGCCGTAA TGCTGGAACG GGAAGGAATG GGAATGGATC AGGGACCACA AGAAATGATT
    GACAGGTATC TTTCTCTGGG AGATCATGAC CGCAAGAGAC TGGAGGATGA TGAGGACAGA
    CTGCTGGCCA CGCTTCTGCA CAATATGATT GCTTACATGC TTATGATGAA GGTCGGTAAA
    AACGACATCA GGAAGAAAGT ACGTCGTCTT ATGGGCAAAT CCCACATTGG CCTCACTCAC
    AGTCAGGAGA TCAATGAAGT CCTGGATCGC CTCGCACATT TAAGCGGTCG GGAGCTTTCT
    ATCAGGCCGA GTGGCAGCAG GCATATTAAA AAACAGACAT TTGTTGTGCA TGCTGGGACA
    GATACAACTG GTGACATATT CTTCATGGAG GTGTGTGATG ATTGCATCGT CTTGCGCAGT
    AATATTGGCA CAGTCTATGA GCGCTGGTGG TATGAGAAGC TCATAAATAT GACTTACTGT
    CCCAAAACTA AAGTGTTGTG TCTGTGGAGG AGAAACGGCC AGGAGACGCA GCTAAACAAG
    TTTTACACTA AGAAGTGTCG GGAATTGTAC TACTGTGTTA AAGACAGCAT GGAGCGTGCT
    GCTGCTCGAC AACAAAGCAT CAAACCAGGT CCAGAGCTTG GAGGCGAGTT TCCGGTTCAG
    GACATGAAGA CAGGGGAGGG TGGTCTCCTC CAAGTCACAC TGGAAGGCAT CAACCTCAAA
    TTTATGCACA GCCAGTTCCT GAAACTAAAG AAGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48914
    Clone ID Related Accession (Same CDS sequence) XM_009303265.2
    Accession Version XM_009303265.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5106bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product MAP kinase-activating death domain protein isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303265.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGTG TCTGGTAAGG
    TTGACTCTGA TCACCCAAAA GCAGATCTTC GCCTCCGATT CTAAGGCTCT GGCTAGCATG
    AGTCTAAACA CACAGCCTAT TCTGAACCTG GAGAAGTTTC AGGATGGACA GGAGTTGTCT
    TTATTGCCCC CCGGTCGTGA CAAAGCTTCC CCGTCCTCCA CTGAGTTCAA CCCACTCATC
    TACGGCAATG ATGTGGACTC AGTTGATGTT GCCACAAGGG TGGCAATGGT GAGGTTCTTC
    AACTCTCCAA ACGTGCTGCA AGGGTTCCAG ATGCACACAC GCACTCTCCG TCTCTTCCCT
    CGTCCTGTGG TGGCCTTTCA GTCCACATCT TTCCTGGCTT CTAGGCCACG ACGAAATGGA
    TTCACAGAAA AGCTCTCCCA CACTCAGGCA GTGGAGTACT ATGGAGAATG GGCTCTCAAC
    CCCACCAACC TGGCTTTCCA AAGGATTCAT AACAATGTGT ATGACCCCTC TCTAATAGGC
    GATAAGCCTA AGTGGTATGC CCACCAGTTA CAGCCAGTAT TCTACCGCGT GTATGATGGA
    AACTCTCATT TAGCGGAGGC CTTGAGTGGA CCCCTGCAGG ATGAGACCAA TGATTCAGAC
    CCAACAGACG ACAGTGGCAG CGACAGTGAA GCATATGATG ACTCCAGCTC CTCCTACTCA
    TCTCTTGGAG ATTTTGTGAA TGAGATGATC AAAGGGGACA TACAGGGAGA CACACCAAAT
    GTTGATCCCC TGACCCATGC TGCACTGGGA GATGCAGATG AGGTTGAGAT CCATGACTTT
    CACGAGTATA AGGGGAACAG CGGTGAACCA GAGCCAGAAG GCCCCACAGA AGCAGCGGAC
    AGTCAGCCCT TGCGGTCCAG CTCTAGCACC ACAGCCAGTT CCAGCCCCAG TACAGTCATA
    CAGGGAGTCA ACCATGAGCA GAAGGAACCT GTTGAGGTGG AGCCCATAGC AAATGTGACT
    TACCCAAGCT CTGCCCCAGT ATTGGGTCCT CCTCCTTTTA CAAGACCCAC CCCTGATCCA
    ACCCTTGTTG ACCCTGCCAA TAAGAAGCGA GAATATGACA ACCCCTATTT TGAGCCCCAG
    TACGGTTTCC CTACAGAGGA AGATGCTGAG ACTGATGAGC AGGAGGAGAG TTACACACCA
    CGATTTAACC AGAATCTCAA TGGCAATAAG CCTTCTCGTC CACTGAGACC CAGCAGCCTG
    AAGCTTCCGG GTGAATCTGA CGGAGAGGGA GACTCCAGAA ATAGCTCTCC TAACTCAACC
    ATCTCCAACA ATAGCAGTGA TGGCTTTGGG GGACTCATGT CCTTTGCCAG TAACTTGTAT
    AAAAACCATG GCACCAGTTT CAGTCTGTCC AGCCTGGCTC TGCCTAATAA AGCGAGAGAG
    AAGAACACGC CGTTTCCCAG CCTCAAAGAC GATGCTGATG ACAGTCCTGA AAGTCCTGTA
    TTTGGGCTAA ATTCTCTAAT GGAGATTGTA ACAGAGATCG GCCCGGGGAG CGGAGAAGGT
    GCCCGAGCCC CCAGAGCACT GGTAGATCAG AAATCCTCTG TTATTAAGCA CAGCCCTACT
    GTGAAGAGAG AGTCTCCGTC TCCTCAAGGC AGAGCCAACA ATACCAGTGA GAACCAGCAG
    TTTCTAAAGG AGGTGGTCCA GAGCGTGCTG GAGGGGCAGG GTGTGGGCTG GCTTAACATG
    AAGAAGGTGC GTCGCCTGCT AGAGAATGAA CAGCTCAGAG TCTTTGTGCT CAGCAAACTC
    AACCGTGCGG TCCAGTCAGA GGAGGACGCC CAGCAGGAAG TTATAAGAGA TGTGGAGATA
    AATAGAAAGG TCTACAAAGG CATGCTGGAT ATTTTGAAGT GCACGGTGTC CAGTTTGGAG
    CATTCATACA CTAATGCTGG CTTAGGGGGC ATGGCCAGTG TCTTCAGTCT GCTAGAGATA
    GCACGCACCC ACTACCAAAC CAAAGATCCA GAGAAGCGGA AACGGAGCCC CACCGAAGGG
    CTTAGCAGTC CAGGCAGTAA GGAGAGCCCA TCGGGCCGAA TGGAGAGTGC CAGGGCGGCA
    GGGGTGCTTC TGGTACCCCG TATCCAATTG CCGCCACCCT CCTCTGGGAA ATCATCACGC
    CAGTTTGATA CCCGGAGTCT GAACGAGGAG AATTTTATTG CCTCCATTGA ATTGTGGAGC
    AAGCACCAGG ATAACAGAAA GCAAAAAGCT TTGGAAAAAG AACAGAGTGG TTTAAATAAA
    CGGAAATCAG GAGCGGATGG AGTAAAACAA CGTGTAGAAG GAGGAGACAC TGAGGAGAAG
    AAATCACAGA TCAGTGCAGA CAGTGGCCTC AGTGTGACAT CTGGCTCACA GAAGAGTGAT
    ACGGATTCCC TGGCGAGCTC TGAACCTCCT GCTCTGACCA GAAGCACCAG CCAGGACTCA
    GAGGCCAGCA CAGTGGTAAG TAACAGCTCA GGTGAGACGC TCGGTGCAGA CAGTGACCTG
    AGCAGCACTG CTGGAGATGG CCTGACAGGA AGACATGGTC AGCATCTCAA CCTGTCCCGT
    GGCACACTGT CTGACAGTGA GATTGAGACC AACCCTGCAA CCAGCTCAGT GTTTGGCAAG
    ACACACAAGC TGAAGCCAGG AGTGAAGGAG CCTGTTGGTG TTAATAAAGC AGCACCTGCT
    CCACCCGTTG AGGATGTTAG CATGAGGATC TATCTGTGTG AGGGGCTTCT GGGCCGGGAT
    AAAAGCTCAG TTTGGGACCA GTTGGAGGAT GCTGCAATGG AAACCTTTTC TCTGAGTAAA
    GAGCGCTCAA CTCTGTGGGA TCAGATGCAG TTCTGGGAGG ACGCCTTCCT GGATGCCGTA
    ATGCTGGAAC GGGAAGGAAT GGGAATGGAT CAGGGACCAC AAGAAATGAT TGACAGGTAT
    CTTTCTCTGG GAGATCATGA CCGCAAGAGA CTGGAGGATG ATGAGGACAG ACTGCTGGCC
    ACGCTTCTGC ACAATATGAT TGCTTACATG CTTATGATGA AGGTCGGTAA AAACGACATC
    AGGAAGAAAG TACGTCGTCT TATGGGCAAA TCCCACATTG GCCTCACTCA CAGTCAGGAG
    ATCAATGAAG TCCTGGATCG CCTCGCACAT TTAAGCGGTC GGGAGCTTTC TATCAGGCCG
    AGTGGCAGCA GGCATATTAA AAAACAGACA TTTGTTGTGC ATGCTGGGAC AGATACAACT
    GGTGACATAT TCTTCATGGA GGTGTGTGAT GATTGCATCG TCTTGCGCAG TAATATTGGC
    ACAGTCTATG AGCGCTGGTG GTATGAGAAG CTCATAAATA TGACTTACTG TCCCAAAACT
    AAAGTGTTGT GTCTGTGGAG GAGAAACGGC CAGGAGACGC AGCTAAACAA GTTTTACACT
    AAGAAGTGTC GGGAATTGTA CTACTGTGTT AAAGACAGCA TGGAGCGTGC TGCTGCTCGA
    CAACAAAGCA TCAAACCAGG TCCAGAGCTT GGAGGCGAGT TTCCGGTTCA GGACATGAAG
    ACAGGGGAGG GTGGTCTCCT CCAAGTCACA CTGGAAGGCA TCAACCTCAA ATTTATGCAC
    AGCCAGGAGC GGAAGGTTTT CATAGAGCTG AATCACATTA AAAAGTGCAA TACAGTGAAA
    GGAGTCTTTG TCCTGGAGGA GTTTGTTCCT GAAACTAAAG AAGTGGTGAT CCATAAGTAC
    AAGACTCCTA TGGCACATCA GATCTGTTAC TCGGTGCTCT GCCTCTTCTC CTACATGGCG
    GCGGTCAAGG GGAAAGAGTC AGAGGGCAAA CCCAAGATGC TGTCCCCTCG TCCACTAGCG
    AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301540.1
    CDS435..5540
    Translation

    Target ORF information:

    RefSeq Version XM_009303265.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303265.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGTG TCTGGTAAGG
    TTGACTCTGA TCACCCAAAA GCAGATCTTC GCCTCCGATT CTAAGGCTCT GGCTAGCATG
    AGTCTAAACA CACAGCCTAT TCTGAACCTG GAGAAGTTTC AGGATGGACA GGAGTTGTCT
    TTATTGCCCC CCGGTCGTGA CAAAGCTTCC CCGTCCTCCA CTGAGTTCAA CCCACTCATC
    TACGGCAATG ATGTGGACTC AGTTGATGTT GCCACAAGGG TGGCAATGGT GAGGTTCTTC
    AACTCTCCAA ACGTGCTGCA AGGGTTCCAG ATGCACACAC GCACTCTCCG TCTCTTCCCT
    CGTCCTGTGG TGGCCTTTCA GTCCACATCT TTCCTGGCTT CTAGGCCACG ACGAAATGGA
    TTCACAGAAA AGCTCTCCCA CACTCAGGCA GTGGAGTACT ATGGAGAATG GGCTCTCAAC
    CCCACCAACC TGGCTTTCCA AAGGATTCAT AACAATGTGT ATGACCCCTC TCTAATAGGC
    GATAAGCCTA AGTGGTATGC CCACCAGTTA CAGCCAGTAT TCTACCGCGT GTATGATGGA
    AACTCTCATT TAGCGGAGGC CTTGAGTGGA CCCCTGCAGG ATGAGACCAA TGATTCAGAC
    CCAACAGACG ACAGTGGCAG CGACAGTGAA GCATATGATG ACTCCAGCTC CTCCTACTCA
    TCTCTTGGAG ATTTTGTGAA TGAGATGATC AAAGGGGACA TACAGGGAGA CACACCAAAT
    GTTGATCCCC TGACCCATGC TGCACTGGGA GATGCAGATG AGGTTGAGAT CCATGACTTT
    CACGAGTATA AGGGGAACAG CGGTGAACCA GAGCCAGAAG GCCCCACAGA AGCAGCGGAC
    AGTCAGCCCT TGCGGTCCAG CTCTAGCACC ACAGCCAGTT CCAGCCCCAG TACAGTCATA
    CAGGGAGTCA ACCATGAGCA GAAGGAACCT GTTGAGGTGG AGCCCATAGC AAATGTGACT
    TACCCAAGCT CTGCCCCAGT ATTGGGTCCT CCTCCTTTTA CAAGACCCAC CCCTGATCCA
    ACCCTTGTTG ACCCTGCCAA TAAGAAGCGA GAATATGACA ACCCCTATTT TGAGCCCCAG
    TACGGTTTCC CTACAGAGGA AGATGCTGAG ACTGATGAGC AGGAGGAGAG TTACACACCA
    CGATTTAACC AGAATCTCAA TGGCAATAAG CCTTCTCGTC CACTGAGACC CAGCAGCCTG
    AAGCTTCCGG GTGAATCTGA CGGAGAGGGA GACTCCAGAA ATAGCTCTCC TAACTCAACC
    ATCTCCAACA ATAGCAGTGA TGGCTTTGGG GGACTCATGT CCTTTGCCAG TAACTTGTAT
    AAAAACCATG GCACCAGTTT CAGTCTGTCC AGCCTGGCTC TGCCTAATAA AGCGAGAGAG
    AAGAACACGC CGTTTCCCAG CCTCAAAGAC GATGCTGATG ACAGTCCTGA AAGTCCTGTA
    TTTGGGCTAA ATTCTCTAAT GGAGATTGTA ACAGAGATCG GCCCGGGGAG CGGAGAAGGT
    GCCCGAGCCC CCAGAGCACT GGTAGATCAG AAATCCTCTG TTATTAAGCA CAGCCCTACT
    GTGAAGAGAG AGTCTCCGTC TCCTCAAGGC AGAGCCAACA ATACCAGTGA GAACCAGCAG
    TTTCTAAAGG AGGTGGTCCA GAGCGTGCTG GAGGGGCAGG GTGTGGGCTG GCTTAACATG
    AAGAAGGTGC GTCGCCTGCT AGAGAATGAA CAGCTCAGAG TCTTTGTGCT CAGCAAACTC
    AACCGTGCGG TCCAGTCAGA GGAGGACGCC CAGCAGGAAG TTATAAGAGA TGTGGAGATA
    AATAGAAAGG TCTACAAAGG CATGCTGGAT ATTTTGAAGT GCACGGTGTC CAGTTTGGAG
    CATTCATACA CTAATGCTGG CTTAGGGGGC ATGGCCAGTG TCTTCAGTCT GCTAGAGATA
    GCACGCACCC ACTACCAAAC CAAAGATCCA GAGAAGCGGA AACGGAGCCC CACCGAAGGG
    CTTAGCAGTC CAGGCAGTAA GGAGAGCCCA TCGGGCCGAA TGGAGAGTGC CAGGGCGGCA
    GGGGTGCTTC TGGTACCCCG TATCCAATTG CCGCCACCCT CCTCTGGGAA ATCATCACGC
    CAGTTTGATA CCCGGAGTCT GAACGAGGAG AATTTTATTG CCTCCATTGA ATTGTGGAGC
    AAGCACCAGG ATAACAGAAA GCAAAAAGCT TTGGAAAAAG AACAGAGTGG TTTAAATAAA
    CGGAAATCAG GAGCGGATGG AGTAAAACAA CGTGTAGAAG GAGGAGACAC TGAGGAGAAG
    AAATCACAGA TCAGTGCAGA CAGTGGCCTC AGTGTGACAT CTGGCTCACA GAAGAGTGAT
    ACGGATTCCC TGGCGAGCTC TGAACCTCCT GCTCTGACCA GAAGCACCAG CCAGGACTCA
    GAGGCCAGCA CAGTGGTAAG TAACAGCTCA GGTGAGACGC TCGGTGCAGA CAGTGACCTG
    AGCAGCACTG CTGGAGATGG CCTGACAGGA AGACATGGTC AGCATCTCAA CCTGTCCCGT
    GGCACACTGT CTGACAGTGA GATTGAGACC AACCCTGCAA CCAGCTCAGT GTTTGGCAAG
    ACACACAAGC TGAAGCCAGG AGTGAAGGAG CCTGTTGGTG TTAATAAAGC AGCACCTGCT
    CCACCCGTTG AGGATGTTAG CATGAGGATC TATCTGTGTG AGGGGCTTCT GGGCCGGGAT
    AAAAGCTCAG TTTGGGACCA GTTGGAGGAT GCTGCAATGG AAACCTTTTC TCTGAGTAAA
    GAGCGCTCAA CTCTGTGGGA TCAGATGCAG TTCTGGGAGG ACGCCTTCCT GGATGCCGTA
    ATGCTGGAAC GGGAAGGAAT GGGAATGGAT CAGGGACCAC AAGAAATGAT TGACAGGTAT
    CTTTCTCTGG GAGATCATGA CCGCAAGAGA CTGGAGGATG ATGAGGACAG ACTGCTGGCC
    ACGCTTCTGC ACAATATGAT TGCTTACATG CTTATGATGA AGGTCGGTAA AAACGACATC
    AGGAAGAAAG TACGTCGTCT TATGGGCAAA TCCCACATTG GCCTCACTCA CAGTCAGGAG
    ATCAATGAAG TCCTGGATCG CCTCGCACAT TTAAGCGGTC GGGAGCTTTC TATCAGGCCG
    AGTGGCAGCA GGCATATTAA AAAACAGACA TTTGTTGTGC ATGCTGGGAC AGATACAACT
    GGTGACATAT TCTTCATGGA GGTGTGTGAT GATTGCATCG TCTTGCGCAG TAATATTGGC
    ACAGTCTATG AGCGCTGGTG GTATGAGAAG CTCATAAATA TGACTTACTG TCCCAAAACT
    AAAGTGTTGT GTCTGTGGAG GAGAAACGGC CAGGAGACGC AGCTAAACAA GTTTTACACT
    AAGAAGTGTC GGGAATTGTA CTACTGTGTT AAAGACAGCA TGGAGCGTGC TGCTGCTCGA
    CAACAAAGCA TCAAACCAGG TCCAGAGCTT GGAGGCGAGT TTCCGGTTCA GGACATGAAG
    ACAGGGGAGG GTGGTCTCCT CCAAGTCACA CTGGAAGGCA TCAACCTCAA ATTTATGCAC
    AGCCAGGAGC GGAAGGTTTT CATAGAGCTG AATCACATTA AAAAGTGCAA TACAGTGAAA
    GGAGTCTTTG TCCTGGAGGA GTTTGTTCCT GAAACTAAAG AAGTGGTGAT CCATAAGTAC
    AAGACTCCTA TGGCACATCA GATCTGTTAC TCGGTGCTCT GCCTCTTCTC CTACATGGCG
    GCGGTCAAGG GGAAAGAGTC AGAGGGCAAA CCCAAGATGC TGTCCCCTCG TCCACTAGCG
    AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48915
    Clone ID Related Accession (Same CDS sequence) XM_009303266.2
    Accession Version XM_009303266.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5097bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product MAP kinase-activating death domain protein isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303266.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGTG TCTGGTAAGG
    TTGACTCTGA TCACCCAAAA GCAGATCTTC GCCTCCGATT CTAAGGCTCT GGCTAGCATG
    AGTCTAAACA CACAGCCTAT TCTGAACCTG GAGAAGTTTC AGGATGGACA GGAGTTGTCT
    TTATTGCCCC CCGGTCGTGA CAAAGCTTCC CCGTCCTCCA CTGAGTTCAA CCCACTCATC
    TACGGCAATG ATGTGGACTC AGTTGATGTT GCCACAAGGG TGGCAATGGT GAGGTTCTTC
    AACTCTCCAA ACGTGCTGCA AGGGTTCCAG ATGCACACAC GCACTCTCCG TCTCTTCCCT
    CGTCCTGTGG TGGCCTTTCA GTCCACATCT TTCCTGGCTT CTAGGCCACG ACGAAATGGA
    TTCACAGAAA AGCTCTCCCA CACTCAGGCA GTGGAGTACT ATGGAGAATG GGCTCTCAAC
    CCCACCAACC TGGCTTTCCA AAGGATTCAT AACAATGTGT ATGACCCCTC TCTAATAGGC
    GATAAGCCTA AGTGGTATGC CCACCAGTTA CAGCCAGTAT TCTACCGCGT GTATGATGGA
    AACTCTCATT TAGCGGAGGC CTTGAGTGGA CCCCTGCAGG ATGAGACCAA TGATTCAGAC
    CCAACAGACG ACAGTGGCAG CGACAGTGAA GCATATGATG ACTCCAGCTC CTCCTACTCA
    TCTCTTGGAG ATTTTGTGAA TGAGATGATC AAAGGGGACA TACAGGGAGA CACACCAAAT
    GTTGATCCCC TGACCCATGC TGCACTGGGA GATGCAGATG AGGTTGAGAT CCATGACTTT
    CACGAGTATA AGGGGAACAG CGGTGAACCA GAGCCAGAAG GCCCCACAGA AGCAGCGGAC
    AGTCAGCCCT TGCGGTCCAG CTCTAGCACC ACAGCCAGTT CCAGCCCCAG TACAGTCATA
    CAGGGAGTCA ACCATGAGCA GAAGGAACCT GTTGAGGTGG AGCCCATAGC AAATGTGACT
    TACCCAAGCT CTGCCCCAGT ATTGGGTCCT CCTCCTTTTA CAAGACCCAC CCCTGATCCA
    ACCCTTGTTG ACCCTGCCAA TAAGAAGCGA GAATATGACA ACCCCTATTT TGAGCCCCAG
    TACGGTTTCC CTACAGAGGA AGATGCTGAG ACTGATGAGC AGGAGGAGAG TTACACACCA
    CGATTTAACC AGAATCTCAA TGGCAATAAG CCTTCTCGTC CACTGAGACC CAGCAGCCTG
    AAGCTTCCGG GTGAATCTGA CGGAGAGGGA GACTCCAGAA ATAGCTCTCC TAACTCAACC
    ATCTCCAACA ATAGCAGTGA TGGCTTTGGG GGACTCATGT CCTTTGCCAG TAACTTGTAT
    AAAAACCATG GCACCAGTTT CAGTCTGTCC AGCCTGGCTC TGCCTAATAA AGCGAGAGAG
    AAGAACACGC CGTTTCCCAG CCTCAAAGAC GATGCTGATG ACAGTCCTGA AAGTCCTGTA
    TTTGGGCTAA ATTCTCTAAT GGAGATTGTA ACAGAGATCG GCCCGGGGAG CGGAGAAGGT
    GCCCGAGCCC CCAGAGCACT GGTAGATCAG AAATCCTCTG TTATTAAGCA CAGCCCTACT
    GTGAAGAGAG AGTCTCCGTC TCCTCAAGGC AGAGCCAACA ATACCAGTGA GAACCAGCAG
    TTTCTAAAGG AGGTGGTCCA GAGCGTGCTG GAGGGGCAGG GTGTGGGCTG GCTTAACATG
    AAGAAGGTGC GTCGCCTGCT AGAGAATGAA CAGCTCAGAG TCTTTGTGCT CAGCAAACTC
    AACCGTGCGG TCCAGTCAGA GGAGGACGCC CAGCAGGAAG TTATAAGAGA TGTGGAGATA
    AATAGAAAGG TCTACAAAGG CATGCTGGAT ATTTTGAAGT GCACGGTGTC CAGTTTGGAG
    CATTCATACA CTAATGCTGG CTTAGGGGGC ATGGCCAGTG TCTTCAGTCT GCTAGAGATA
    GCACGCACCC ACTACCAAAC CAAAGATCCA GAGAAGCGGA AACGGAGCCC CACCGAAGGG
    CTTAGCAGTC CAGGCAGTAA GGAGAGCCCA TCGGGCCGAA TGGAGAGTGC CAGGGCGGCA
    GGGGTGCTTC TGGTACCCCG TATCCAATTG CCGCCACCCT CCTCTGGGAA ATCATCACGC
    CAGTTTGATA CCCGGAGTCT GAACGAGGAG AATTTTATTG CCTCCATTGA ATTGTGGAGC
    AAGCACCAGG ATAACAGAAA GCAAAAAGCT TTGGAAAAAG AACAGAGTGG TTTAAATAAA
    CGGAAATCAG GAGCGGATGG AGTAAAACAA CGTGTAGAAG GAGGAGACAC TGAGGAGAAG
    AAATCACAGA TCAGTGCAGA CAGTGGCCTC AGTGTGACAT CTGGCTCACA GAAGAGTGAT
    ACGGATTCCC TGGCGAGCTC TGAACCTCCT GCTCTGACCA GAAGCACCAG CCAGGACTCA
    GAGGCCAGCA CAGTGGTAAG TAACAGCTCA GGTGAGACGC TCGGTGCAGA CAGTGACCTG
    AGCAGCACTG CTGGAGATGG CCTGACAGGA AGACATGGTC AGCATCTCAA CCTGTCCCGT
    GGCACACTGT CTGACAGTGA GATTGAGACC AACCCTGCAA CCAGCTCAGT GTTTGGCAAG
    ACACACAAGC TGAAGCCAGG AGTGAAGGAG CCTGTTGGTG TTAATAAAGC AGCACCTGCT
    CCACCCGTTG AGGATGTTAG CATGAGGATC TATCTGTGTG AGGGGCTTCT GGGCCGGGAT
    AAAAGCTCAG TTTGGGACCA GTTGGAGGAT GCTGCAATGG AAACCTTTTC TCTGAGTAAA
    GAGCGCTCAA CTCTGTGGGA TCAGATGCAG TTCTGGGAGG ACGCCTTCCT GGATGCCGTA
    ATGCTGGAAC GGGAAGGAAT GGGAATGGAT CAGGGACCAC AAGAAATGAT TGACAGGTAT
    CTTTCTCTGG GAGATCATGA CCGCAAGAGA CTGGAGGATG ATGAGGACAG ACTGCTGGCC
    ACGCTTCTGC ACAATATGAT TGCTTACATG CTTATGATGA AGGTCGGTAA AAACGACATC
    AGGAAGAAAG TACGTCGTCT TATGGGCAAA TCCCACATTG GCCTCACTCA CAGTCAGGAG
    ATCAATGAAG TCCTGGATCG CCTCGCACAT TTAAGCGGTC GGGAGCTTTC TATCAGGCCG
    AGTGGCAGCA GGCATATTAA AAAACAGACA TTTGTTGTGC ATGCTGGGAC AGATACAACT
    GGTGACATAT TCTTCATGGA GGTGTGTGAT GATTGCATCG TCTTGCGCAG TAATATTGGC
    ACAGTCTATG AGCGCTGGTG GTATGAGAAG CTCATAAATA TGACTTACTG TCCCAAAACT
    AAAGTGTTGT GTCTGTGGAG GAGAAACGGC CAGGAGACGC AGCTAAACAA GTTTTACACT
    AAGAAGTGTC GGGAATTGTA CTACTGTGTT AAAGACAGCA TGGAGCGTGC TGCTGCTCGA
    CAACAAAGCA TCAAACCAGG TCCAGAGCTT GGAGGCGAGT TTCCGGTTCA GGACATGAAG
    ACAGGGGAGG GTGGTCTCCT CCAAGTCACA CTGGAAGGCA TCAACCTCAA ATTTATGCAC
    AGCCAGGTTT TCATAGAGCT GAATCACATT AAAAAGTGCA ATACAGTGAA AGGAGTCTTT
    GTCCTGGAGG AGTTTGTTCC TGAAACTAAA GAAGTGGTGA TCCATAAGTA CAAGACTCCT
    ATGGCACATC AGATCTGTTA CTCGGTGCTC TGCCTCTTCT CCTACATGGC GGCGGTCAAG
    GGGAAAGAGT CAGAGGGCAA ACCCAAGATG CTGTCCCCTC GTCCACTAGC GAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301541.1
    CDS435..5531
    Translation

    Target ORF information:

    RefSeq Version XM_009303266.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303266.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGTG TCTGGTAAGG
    TTGACTCTGA TCACCCAAAA GCAGATCTTC GCCTCCGATT CTAAGGCTCT GGCTAGCATG
    AGTCTAAACA CACAGCCTAT TCTGAACCTG GAGAAGTTTC AGGATGGACA GGAGTTGTCT
    TTATTGCCCC CCGGTCGTGA CAAAGCTTCC CCGTCCTCCA CTGAGTTCAA CCCACTCATC
    TACGGCAATG ATGTGGACTC AGTTGATGTT GCCACAAGGG TGGCAATGGT GAGGTTCTTC
    AACTCTCCAA ACGTGCTGCA AGGGTTCCAG ATGCACACAC GCACTCTCCG TCTCTTCCCT
    CGTCCTGTGG TGGCCTTTCA GTCCACATCT TTCCTGGCTT CTAGGCCACG ACGAAATGGA
    TTCACAGAAA AGCTCTCCCA CACTCAGGCA GTGGAGTACT ATGGAGAATG GGCTCTCAAC
    CCCACCAACC TGGCTTTCCA AAGGATTCAT AACAATGTGT ATGACCCCTC TCTAATAGGC
    GATAAGCCTA AGTGGTATGC CCACCAGTTA CAGCCAGTAT TCTACCGCGT GTATGATGGA
    AACTCTCATT TAGCGGAGGC CTTGAGTGGA CCCCTGCAGG ATGAGACCAA TGATTCAGAC
    CCAACAGACG ACAGTGGCAG CGACAGTGAA GCATATGATG ACTCCAGCTC CTCCTACTCA
    TCTCTTGGAG ATTTTGTGAA TGAGATGATC AAAGGGGACA TACAGGGAGA CACACCAAAT
    GTTGATCCCC TGACCCATGC TGCACTGGGA GATGCAGATG AGGTTGAGAT CCATGACTTT
    CACGAGTATA AGGGGAACAG CGGTGAACCA GAGCCAGAAG GCCCCACAGA AGCAGCGGAC
    AGTCAGCCCT TGCGGTCCAG CTCTAGCACC ACAGCCAGTT CCAGCCCCAG TACAGTCATA
    CAGGGAGTCA ACCATGAGCA GAAGGAACCT GTTGAGGTGG AGCCCATAGC AAATGTGACT
    TACCCAAGCT CTGCCCCAGT ATTGGGTCCT CCTCCTTTTA CAAGACCCAC CCCTGATCCA
    ACCCTTGTTG ACCCTGCCAA TAAGAAGCGA GAATATGACA ACCCCTATTT TGAGCCCCAG
    TACGGTTTCC CTACAGAGGA AGATGCTGAG ACTGATGAGC AGGAGGAGAG TTACACACCA
    CGATTTAACC AGAATCTCAA TGGCAATAAG CCTTCTCGTC CACTGAGACC CAGCAGCCTG
    AAGCTTCCGG GTGAATCTGA CGGAGAGGGA GACTCCAGAA ATAGCTCTCC TAACTCAACC
    ATCTCCAACA ATAGCAGTGA TGGCTTTGGG GGACTCATGT CCTTTGCCAG TAACTTGTAT
    AAAAACCATG GCACCAGTTT CAGTCTGTCC AGCCTGGCTC TGCCTAATAA AGCGAGAGAG
    AAGAACACGC CGTTTCCCAG CCTCAAAGAC GATGCTGATG ACAGTCCTGA AAGTCCTGTA
    TTTGGGCTAA ATTCTCTAAT GGAGATTGTA ACAGAGATCG GCCCGGGGAG CGGAGAAGGT
    GCCCGAGCCC CCAGAGCACT GGTAGATCAG AAATCCTCTG TTATTAAGCA CAGCCCTACT
    GTGAAGAGAG AGTCTCCGTC TCCTCAAGGC AGAGCCAACA ATACCAGTGA GAACCAGCAG
    TTTCTAAAGG AGGTGGTCCA GAGCGTGCTG GAGGGGCAGG GTGTGGGCTG GCTTAACATG
    AAGAAGGTGC GTCGCCTGCT AGAGAATGAA CAGCTCAGAG TCTTTGTGCT CAGCAAACTC
    AACCGTGCGG TCCAGTCAGA GGAGGACGCC CAGCAGGAAG TTATAAGAGA TGTGGAGATA
    AATAGAAAGG TCTACAAAGG CATGCTGGAT ATTTTGAAGT GCACGGTGTC CAGTTTGGAG
    CATTCATACA CTAATGCTGG CTTAGGGGGC ATGGCCAGTG TCTTCAGTCT GCTAGAGATA
    GCACGCACCC ACTACCAAAC CAAAGATCCA GAGAAGCGGA AACGGAGCCC CACCGAAGGG
    CTTAGCAGTC CAGGCAGTAA GGAGAGCCCA TCGGGCCGAA TGGAGAGTGC CAGGGCGGCA
    GGGGTGCTTC TGGTACCCCG TATCCAATTG CCGCCACCCT CCTCTGGGAA ATCATCACGC
    CAGTTTGATA CCCGGAGTCT GAACGAGGAG AATTTTATTG CCTCCATTGA ATTGTGGAGC
    AAGCACCAGG ATAACAGAAA GCAAAAAGCT TTGGAAAAAG AACAGAGTGG TTTAAATAAA
    CGGAAATCAG GAGCGGATGG AGTAAAACAA CGTGTAGAAG GAGGAGACAC TGAGGAGAAG
    AAATCACAGA TCAGTGCAGA CAGTGGCCTC AGTGTGACAT CTGGCTCACA GAAGAGTGAT
    ACGGATTCCC TGGCGAGCTC TGAACCTCCT GCTCTGACCA GAAGCACCAG CCAGGACTCA
    GAGGCCAGCA CAGTGGTAAG TAACAGCTCA GGTGAGACGC TCGGTGCAGA CAGTGACCTG
    AGCAGCACTG CTGGAGATGG CCTGACAGGA AGACATGGTC AGCATCTCAA CCTGTCCCGT
    GGCACACTGT CTGACAGTGA GATTGAGACC AACCCTGCAA CCAGCTCAGT GTTTGGCAAG
    ACACACAAGC TGAAGCCAGG AGTGAAGGAG CCTGTTGGTG TTAATAAAGC AGCACCTGCT
    CCACCCGTTG AGGATGTTAG CATGAGGATC TATCTGTGTG AGGGGCTTCT GGGCCGGGAT
    AAAAGCTCAG TTTGGGACCA GTTGGAGGAT GCTGCAATGG AAACCTTTTC TCTGAGTAAA
    GAGCGCTCAA CTCTGTGGGA TCAGATGCAG TTCTGGGAGG ACGCCTTCCT GGATGCCGTA
    ATGCTGGAAC GGGAAGGAAT GGGAATGGAT CAGGGACCAC AAGAAATGAT TGACAGGTAT
    CTTTCTCTGG GAGATCATGA CCGCAAGAGA CTGGAGGATG ATGAGGACAG ACTGCTGGCC
    ACGCTTCTGC ACAATATGAT TGCTTACATG CTTATGATGA AGGTCGGTAA AAACGACATC
    AGGAAGAAAG TACGTCGTCT TATGGGCAAA TCCCACATTG GCCTCACTCA CAGTCAGGAG
    ATCAATGAAG TCCTGGATCG CCTCGCACAT TTAAGCGGTC GGGAGCTTTC TATCAGGCCG
    AGTGGCAGCA GGCATATTAA AAAACAGACA TTTGTTGTGC ATGCTGGGAC AGATACAACT
    GGTGACATAT TCTTCATGGA GGTGTGTGAT GATTGCATCG TCTTGCGCAG TAATATTGGC
    ACAGTCTATG AGCGCTGGTG GTATGAGAAG CTCATAAATA TGACTTACTG TCCCAAAACT
    AAAGTGTTGT GTCTGTGGAG GAGAAACGGC CAGGAGACGC AGCTAAACAA GTTTTACACT
    AAGAAGTGTC GGGAATTGTA CTACTGTGTT AAAGACAGCA TGGAGCGTGC TGCTGCTCGA
    CAACAAAGCA TCAAACCAGG TCCAGAGCTT GGAGGCGAGT TTCCGGTTCA GGACATGAAG
    ACAGGGGAGG GTGGTCTCCT CCAAGTCACA CTGGAAGGCA TCAACCTCAA ATTTATGCAC
    AGCCAGGTTT TCATAGAGCT GAATCACATT AAAAAGTGCA ATACAGTGAA AGGAGTCTTT
    GTCCTGGAGG AGTTTGTTCC TGAAACTAAA GAAGTGGTGA TCCATAAGTA CAAGACTCCT
    ATGGCACATC AGATCTGTTA CTCGGTGCTC TGCCTCTTCT CCTACATGGC GGCGGTCAAG
    GGGAAAGAGT CAGAGGGCAA ACCCAAGATG CTGTCCCCTC GTCCACTAGC GAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48916
    Clone ID Related Accession (Same CDS sequence) XM_009303267.2
    Accession Version XM_009303267.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5082bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product MAP kinase-activating death domain protein isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303267.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGTG TCTGGTAAGG
    TTGACTCTGA TCACCCAAAA GCAGATCTTC GCCTCCGATT CTAAGGCTCT GGCTAGCATG
    AGTCTAAACA CACAGCCTAT TCTGAACCTG GAGAAGTTTC AGGATGGACA GGAGTTGTCT
    TTATTGCCCC CCGGTCGTGA CAAAGCTTCC CCGTCCTCCA CTGAGTTCAA CCCACTCATC
    TACGGCAATG ATGTGGACTC AGTTGATGTT GCCACAAGGG TGGCAATGGT GAGGTTCTTC
    AACTCTCCAA ACGTGCTGCA AGGGTTCCAG ATGCACACAC GCACTCTCCG TCTCTTCCCT
    CGTCCTGTGG TGGCCTTTCA GTCCACATCT TTCCTGGCTT CTAGGCCACG ACGAAATGGA
    TTCACAGAAA AGCTCTCCCA CACTCAGGCA GTGGAGTACT ATGGAGAATG GGCTCTCAAC
    CCCACCAACC TGGCTTTCCA AAGGATTCAT AACAATGTGT ATGACCCCTC TCTAATAGGC
    GATAAGCCTA AGTGGTATGC CCACCAGTTA CAGCCAGTAT TCTACCGCGT GTATGATGGA
    AACTCTCATT TAGCGGAGGC CTTGAGTGGA CCCCTGCAGG ATGAGACCAA TGATTCAGAC
    CCAACAGACG ACAGTGGCAG CGACAGTGAA GCATATGATG ACTCCAGCTC CTCCTACTCA
    TCTCTTGGAG ATTTTGTGAA TGAGATGATC AAAGGGGACA TACAGGGAGA CACACCAAAT
    GTTGATCCCC TGACCCATGC TGCACTGGGA GATGCAGATG AGGTTGAGAT CCATGACTTT
    CACGAGTATA AGGGGAACAG CGGTGAACCA GAGCCAGAAG GCCCCACAGA AGCAGCGGAC
    AGTCAGCCCT TGCGGTCCAG CTCTAGCACC ACAGCCAGTT CCAGCCCCAG TACAGTCATA
    CAGGGAGTCA ACCATGAGCA GAAGGAACCT GTTGAGGTGG AGCCCATAGC AAATGTGACT
    TACCCAAGCT CTGCCCCAGT ATTGGGTCCT CCTCCTTTTA CAAGACCCAC CCCTGATCCA
    ACCCTTGTTG ACCCTGCCAA TAAGAAGCGA GAATATGACA ACCCCTATTT TGAGCCCCAG
    TACGGTTTCC CTACAGAGGA AGATGCTGAG ACTGATGAGC AGGAGGAGAG TTACACACCA
    CGATTTAACC AGAATCTCAA TGGCAATAAG CCTTCTCGTC CACTGAGACC CAGCAGCCTG
    AAGCTTCCGG GTGAATCTGA CGGAGAGGGA GACTCCAGAA ATAGCTCTCC TAACTCAACC
    ATCTCCAACA ATAGCAGTGA TGGCTTTGGG GGACTCATGT CCTTTGCCAG TAACTTGTAT
    AAAAACCATG GCACCAGTTT CAGTCTGTCC AGCCTGGCTC TGCCTAATAA AGCGAGAGAG
    AAGAACACGC CGTTTCCCAG CCTCAAAGAC GATGCTGATG ACAGTCCTGA AAGTCCTGTA
    TTTGGGCTAA ATTCTCTAAT GGAGATTGTA ACAGAGATCG GCCCGGGGAG CGGAGAAGGT
    GCCCGAGCCC CCAGAGCACT GGTAGATCAG AAATCCTCTG TTATTAAGCA CAGCCCTACT
    GTGAAGAGAG AGTCTCCGTC TCCTCAAGGC AGAGCCAACA ATACCAGTGA GAACCAGCAG
    TTTCTAAAGG AGGTGGTCCA GAGCGTGCTG GAGGGGCAGG GTGTGGGCTG GCTTAACATG
    AAGAAGGTGC GTCGCCTGCT AGAGAATGAA CAGCTCAGAG TCTTTGTGCT CAGCAAACTC
    AACCGTGCGG TCCAGTCAGA GGAGGACGCC CAGCAGGAAG TTATAAGAGA TGTGGAGATA
    AATAGAAAGG TCTACAAAGG CATGCTGGAT ATTTTGAAGT GCACGGTGTC CAGTTTGGAG
    CATTCATACA CTAATGCTGG CTTAGGGGGC ATGGCCAGTG TCTTCAGTCT GCTAGAGATA
    GCACGCACCC ACTACCAAAC CAAAGATCCA GAGAAGCGGA AACGGAGCCC CACCGAAGGG
    CTTAGCAGTC CAGGCAGTAA GGAGAGCCCA TCGGGCCGAA TGGAGAGTGC CAGGGCGGCA
    GGGGTGCTTC TGGTACCCCG TATCCAATTG CCGCCACCCT CCTCTGGGAA ATCATCACGC
    CAGTTTGATA CCCGGAGTCT GAACGAGGAG AATTTTATTG CCTCCATTGA ATTGTGGAGC
    AAGCACCAGG ATAACAGAAA GCAAAAAGCT TTGGAAAAAG AACAGAGAGC GGATGGAGTA
    AAACAACGTG TAGAAGGAGG AGACACTGAG GAGAAGAAAT CACAGATCAG TGCAGACAGT
    GGCCTCAGTG TGACATCTGG CTCACAGAAG AGTGATACGG ATTCCCTGGC GAGCTCTGAA
    CCTCCTGCTC TGACCAGAAG CACCAGCCAG GACTCAGAGG CCAGCACAGT GGTAAGTAAC
    AGCTCAGGTG AGACGCTCGG TGCAGACAGT GACCTGAGCA GCACTGCTGG AGATGGCCTG
    ACAGGAAGAC ATGGTCAGCA TCTCAACCTG TCCCGTGGCA CACTGTCTGA CAGTGAGATT
    GAGACCAACC CTGCAACCAG CTCAGTGTTT GGCAAGACAC ACAAGCTGAA GCCAGGAGTG
    AAGGAGCCTG TTGGTGTTAA TAAAGCAGCA CCTGCTCCAC CCGTTGAGGA TGTTAGCATG
    AGGATCTATC TGTGTGAGGG GCTTCTGGGC CGGGATAAAA GCTCAGTTTG GGACCAGTTG
    GAGGATGCTG CAATGGAAAC CTTTTCTCTG AGTAAAGAGC GCTCAACTCT GTGGGATCAG
    ATGCAGTTCT GGGAGGACGC CTTCCTGGAT GCCGTAATGC TGGAACGGGA AGGAATGGGA
    ATGGATCAGG GACCACAAGA AATGATTGAC AGGTATCTTT CTCTGGGAGA TCATGACCGC
    AAGAGACTGG AGGATGATGA GGACAGACTG CTGGCCACGC TTCTGCACAA TATGATTGCT
    TACATGCTTA TGATGAAGGT CGGTAAAAAC GACATCAGGA AGAAAGTACG TCGTCTTATG
    GGCAAATCCC ACATTGGCCT CACTCACAGT CAGGAGATCA ATGAAGTCCT GGATCGCCTC
    GCACATTTAA GCGGTCGGGA GCTTTCTATC AGGCCGAGTG GCAGCAGGCA TATTAAAAAA
    CAGACATTTG TTGTGCATGC TGGGACAGAT ACAACTGGTG ACATATTCTT CATGGAGGTG
    TGTGATGATT GCATCGTCTT GCGCAGTAAT ATTGGCACAG TCTATGAGCG CTGGTGGTAT
    GAGAAGCTCA TAAATATGAC TTACTGTCCC AAAACTAAAG TGTTGTGTCT GTGGAGGAGA
    AACGGCCAGG AGACGCAGCT AAACAAGTTT TACACTAAGA AGTGTCGGGA ATTGTACTAC
    TGTGTTAAAG ACAGCATGGA GCGTGCTGCT GCTCGACAAC AAAGCATCAA ACCAGGTCCA
    GAGCTTGGAG GCGAGTTTCC GGTTCAGGAC ATGAAGACAG GGGAGGGTGG TCTCCTCCAA
    GTCACACTGG AAGGCATCAA CCTCAAATTT ATGCACAGCC AGGAGCGGAA GGTTTTCATA
    GAGCTGAATC ACATTAAAAA GTGCAATACA GTGAAAGGAG TCTTTGTCCT GGAGGAGTTT
    GTTCCTGAAA CTAAAGAAGT GGTGATCCAT AAGTACAAGA CTCCTATGGC ACATCAGATC
    TGTTACTCGG TGCTCTGCCT CTTCTCCTAC ATGGCGGCGG TCAAGGGGAA AGAGTCAGAG
    GGCAAACCCA AGATGCTGTC CCCTCGTCCA CTAGCGAGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301542.1
    CDS435..5516
    Translation

    Target ORF information:

    RefSeq Version XM_009303267.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303267.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGTG TCTGGTAAGG
    TTGACTCTGA TCACCCAAAA GCAGATCTTC GCCTCCGATT CTAAGGCTCT GGCTAGCATG
    AGTCTAAACA CACAGCCTAT TCTGAACCTG GAGAAGTTTC AGGATGGACA GGAGTTGTCT
    TTATTGCCCC CCGGTCGTGA CAAAGCTTCC CCGTCCTCCA CTGAGTTCAA CCCACTCATC
    TACGGCAATG ATGTGGACTC AGTTGATGTT GCCACAAGGG TGGCAATGGT GAGGTTCTTC
    AACTCTCCAA ACGTGCTGCA AGGGTTCCAG ATGCACACAC GCACTCTCCG TCTCTTCCCT
    CGTCCTGTGG TGGCCTTTCA GTCCACATCT TTCCTGGCTT CTAGGCCACG ACGAAATGGA
    TTCACAGAAA AGCTCTCCCA CACTCAGGCA GTGGAGTACT ATGGAGAATG GGCTCTCAAC
    CCCACCAACC TGGCTTTCCA AAGGATTCAT AACAATGTGT ATGACCCCTC TCTAATAGGC
    GATAAGCCTA AGTGGTATGC CCACCAGTTA CAGCCAGTAT TCTACCGCGT GTATGATGGA
    AACTCTCATT TAGCGGAGGC CTTGAGTGGA CCCCTGCAGG ATGAGACCAA TGATTCAGAC
    CCAACAGACG ACAGTGGCAG CGACAGTGAA GCATATGATG ACTCCAGCTC CTCCTACTCA
    TCTCTTGGAG ATTTTGTGAA TGAGATGATC AAAGGGGACA TACAGGGAGA CACACCAAAT
    GTTGATCCCC TGACCCATGC TGCACTGGGA GATGCAGATG AGGTTGAGAT CCATGACTTT
    CACGAGTATA AGGGGAACAG CGGTGAACCA GAGCCAGAAG GCCCCACAGA AGCAGCGGAC
    AGTCAGCCCT TGCGGTCCAG CTCTAGCACC ACAGCCAGTT CCAGCCCCAG TACAGTCATA
    CAGGGAGTCA ACCATGAGCA GAAGGAACCT GTTGAGGTGG AGCCCATAGC AAATGTGACT
    TACCCAAGCT CTGCCCCAGT ATTGGGTCCT CCTCCTTTTA CAAGACCCAC CCCTGATCCA
    ACCCTTGTTG ACCCTGCCAA TAAGAAGCGA GAATATGACA ACCCCTATTT TGAGCCCCAG
    TACGGTTTCC CTACAGAGGA AGATGCTGAG ACTGATGAGC AGGAGGAGAG TTACACACCA
    CGATTTAACC AGAATCTCAA TGGCAATAAG CCTTCTCGTC CACTGAGACC CAGCAGCCTG
    AAGCTTCCGG GTGAATCTGA CGGAGAGGGA GACTCCAGAA ATAGCTCTCC TAACTCAACC
    ATCTCCAACA ATAGCAGTGA TGGCTTTGGG GGACTCATGT CCTTTGCCAG TAACTTGTAT
    AAAAACCATG GCACCAGTTT CAGTCTGTCC AGCCTGGCTC TGCCTAATAA AGCGAGAGAG
    AAGAACACGC CGTTTCCCAG CCTCAAAGAC GATGCTGATG ACAGTCCTGA AAGTCCTGTA
    TTTGGGCTAA ATTCTCTAAT GGAGATTGTA ACAGAGATCG GCCCGGGGAG CGGAGAAGGT
    GCCCGAGCCC CCAGAGCACT GGTAGATCAG AAATCCTCTG TTATTAAGCA CAGCCCTACT
    GTGAAGAGAG AGTCTCCGTC TCCTCAAGGC AGAGCCAACA ATACCAGTGA GAACCAGCAG
    TTTCTAAAGG AGGTGGTCCA GAGCGTGCTG GAGGGGCAGG GTGTGGGCTG GCTTAACATG
    AAGAAGGTGC GTCGCCTGCT AGAGAATGAA CAGCTCAGAG TCTTTGTGCT CAGCAAACTC
    AACCGTGCGG TCCAGTCAGA GGAGGACGCC CAGCAGGAAG TTATAAGAGA TGTGGAGATA
    AATAGAAAGG TCTACAAAGG CATGCTGGAT ATTTTGAAGT GCACGGTGTC CAGTTTGGAG
    CATTCATACA CTAATGCTGG CTTAGGGGGC ATGGCCAGTG TCTTCAGTCT GCTAGAGATA
    GCACGCACCC ACTACCAAAC CAAAGATCCA GAGAAGCGGA AACGGAGCCC CACCGAAGGG
    CTTAGCAGTC CAGGCAGTAA GGAGAGCCCA TCGGGCCGAA TGGAGAGTGC CAGGGCGGCA
    GGGGTGCTTC TGGTACCCCG TATCCAATTG CCGCCACCCT CCTCTGGGAA ATCATCACGC
    CAGTTTGATA CCCGGAGTCT GAACGAGGAG AATTTTATTG CCTCCATTGA ATTGTGGAGC
    AAGCACCAGG ATAACAGAAA GCAAAAAGCT TTGGAAAAAG AACAGAGAGC GGATGGAGTA
    AAACAACGTG TAGAAGGAGG AGACACTGAG GAGAAGAAAT CACAGATCAG TGCAGACAGT
    GGCCTCAGTG TGACATCTGG CTCACAGAAG AGTGATACGG ATTCCCTGGC GAGCTCTGAA
    CCTCCTGCTC TGACCAGAAG CACCAGCCAG GACTCAGAGG CCAGCACAGT GGTAAGTAAC
    AGCTCAGGTG AGACGCTCGG TGCAGACAGT GACCTGAGCA GCACTGCTGG AGATGGCCTG
    ACAGGAAGAC ATGGTCAGCA TCTCAACCTG TCCCGTGGCA CACTGTCTGA CAGTGAGATT
    GAGACCAACC CTGCAACCAG CTCAGTGTTT GGCAAGACAC ACAAGCTGAA GCCAGGAGTG
    AAGGAGCCTG TTGGTGTTAA TAAAGCAGCA CCTGCTCCAC CCGTTGAGGA TGTTAGCATG
    AGGATCTATC TGTGTGAGGG GCTTCTGGGC CGGGATAAAA GCTCAGTTTG GGACCAGTTG
    GAGGATGCTG CAATGGAAAC CTTTTCTCTG AGTAAAGAGC GCTCAACTCT GTGGGATCAG
    ATGCAGTTCT GGGAGGACGC CTTCCTGGAT GCCGTAATGC TGGAACGGGA AGGAATGGGA
    ATGGATCAGG GACCACAAGA AATGATTGAC AGGTATCTTT CTCTGGGAGA TCATGACCGC
    AAGAGACTGG AGGATGATGA GGACAGACTG CTGGCCACGC TTCTGCACAA TATGATTGCT
    TACATGCTTA TGATGAAGGT CGGTAAAAAC GACATCAGGA AGAAAGTACG TCGTCTTATG
    GGCAAATCCC ACATTGGCCT CACTCACAGT CAGGAGATCA ATGAAGTCCT GGATCGCCTC
    GCACATTTAA GCGGTCGGGA GCTTTCTATC AGGCCGAGTG GCAGCAGGCA TATTAAAAAA
    CAGACATTTG TTGTGCATGC TGGGACAGAT ACAACTGGTG ACATATTCTT CATGGAGGTG
    TGTGATGATT GCATCGTCTT GCGCAGTAAT ATTGGCACAG TCTATGAGCG CTGGTGGTAT
    GAGAAGCTCA TAAATATGAC TTACTGTCCC AAAACTAAAG TGTTGTGTCT GTGGAGGAGA
    AACGGCCAGG AGACGCAGCT AAACAAGTTT TACACTAAGA AGTGTCGGGA ATTGTACTAC
    TGTGTTAAAG ACAGCATGGA GCGTGCTGCT GCTCGACAAC AAAGCATCAA ACCAGGTCCA
    GAGCTTGGAG GCGAGTTTCC GGTTCAGGAC ATGAAGACAG GGGAGGGTGG TCTCCTCCAA
    GTCACACTGG AAGGCATCAA CCTCAAATTT ATGCACAGCC AGGAGCGGAA GGTTTTCATA
    GAGCTGAATC ACATTAAAAA GTGCAATACA GTGAAAGGAG TCTTTGTCCT GGAGGAGTTT
    GTTCCTGAAA CTAAAGAAGT GGTGATCCAT AAGTACAAGA CTCCTATGGC ACATCAGATC
    TGTTACTCGG TGCTCTGCCT CTTCTCCTAC ATGGCGGCGG TCAAGGGGAA AGAGTCAGAG
    GGCAAACCCA AGATGCTGTC CCCTCGTCCA CTAGCGAGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48917
    Clone ID Related Accession (Same CDS sequence) XM_009303268.2
    Accession Version XM_009303268.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5076bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product MAP kinase-activating death domain protein isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303268.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGTG TCTGGTAAGG
    TTGACTCTGA TCACCCAAAA GCAGATCTTC GCCTCCGATT CTAAGGCTCT GGCTAGCATG
    AGTCTAAACA CACAGCCTAT TCTGAACCTG GAGAAGTTTC AGGATGGACA GGAGTTGTCT
    TTATTGCCCC CCGGTCGTGA CAAAGCTTCC CCGTCCTCCA CTGAGTTCAA CCCACTCATC
    TACGGCAATG ATGTGGACTC AGTTGATGTT GCCACAAGGG TGGCAATGGT GAGGTTCTTC
    AACTCTCCAA ACGTGCTGCA AGGGTTCCAG ATGCACACAC GCACTCTCCG TCTCTTCCCT
    CGTCCTGTGG TGGCCTTTCA GTCCACATCT TTCCTGGCTT CTAGGCCACG ACGAAATGGA
    TTCACAGAAA AGCTCTCCCA CACTCAGGCA GTGGAGTACT ATGGAGAATG GGCTCTCAAC
    CCCACCAACC TGGCTTTCCA AAGGATTCAT AACAATGTGT ATGACCCCTC TCTAATAGGC
    GATAAGCCTA AGTGGTATGC CCACCAGTTA CAGCCAGTAT TCTACCGCGT GTATGATGGA
    AACTCTCATT TAGCGGAGGC CTTGAGTGGA CCCCTGCAGG ATGAGACCAA TGATTCAGAC
    CCAACAGACG ACAGTGGCAG CGACAGTGAA GCATATGATG ACTCCAGCTC CTCCTACTCA
    TCTCTTGGAG ATTTTGTGAA TGAGATGATC AAAGGGGACA TACAGGGAGA CACACCAAAT
    GTTGATCCCC TGACCCATGC TGCACTGGGA GATGCAGATG AGGTTGAGAT CCATGACTTT
    CACGAGTATA AGGGGAACAG CGGTGAACCA GAGCCAGAAG GCCCCACAGA AGCAGCGGAC
    AGTCAGCCCT TGCGGTCCAG CTCTAGCACC ACAGCCAGTT CCAGCCCCAG TACAGTCATA
    CAGGGAGTCA ACCATGAGCA GAAGGAACCT GTTGAGGTGG AGCCCATAGC AAATGTGACT
    TACCCAAGCT CTGCCCCAGT ATTGGGTCCT CCTCCTTTTA CAAGACCCAC CCCTGATCCA
    ACCCTTGTTG ACCCTGCCAA TAAGAAGCGA GAATATGACA ACCCCTATTT TGAGCCCCAG
    TACGGTTTCC CTACAGAGGA AGATGCTGAG ACTGATGAGC AGGAGGAGAG TTACACACCA
    CGATTTAACC AGAATCTCAA TGGCAATAAG CCTTCTCGTC CACTGAGACC CAGCAGCCTG
    AAGCTTCCGG GTGAATCTGA CGGAGAGGGA GACTCCAGAA ATAGCTCTCC TAACTCAACC
    ATCTCCAACA ATAGCAGTGA TGGCTTTGGG GGACTCATGT CCTTTGCCAG TAACTTGTAT
    AAAAACCATG GCACCAGTTT CAGTCTGTCC AGCCTGGCTC TGCCTAATAA AGCGAGAGAG
    AAGAACACGC CGTTTCCCAG CCTCAAAGTA TTTGGGCTAA ATTCTCTAAT GGAGATTGTA
    ACAGAGATCG GCCCGGGGAG CGGAGAAGGT GCCCGAGCCC CCAGAGCACT GGTAGATCAG
    AAATCCTCTG TTATTAAGCA CAGCCCTACT GTGAAGAGAG AGTCTCCGTC TCCTCAAGGC
    AGAGCCAACA ATACCAGTGA GAACCAGCAG TTTCTAAAGG AGGTGGTCCA GAGCGTGCTG
    GAGGGGCAGG GTGTGGGCTG GCTTAACATG AAGAAGGTGC GTCGCCTGCT AGAGAATGAA
    CAGCTCAGAG TCTTTGTGCT CAGCAAACTC AACCGTGCGG TCCAGTCAGA GGAGGACGCC
    CAGCAGGAAG TTATAAGAGA TGTGGAGATA AATAGAAAGG TCTACAAAGG CATGCTGGAT
    ATTTTGAAGT GCACGGTGTC CAGTTTGGAG CATTCATACA CTAATGCTGG CTTAGGGGGC
    ATGGCCAGTG TCTTCAGTCT GCTAGAGATA GCACGCACCC ACTACCAAAC CAAAGATCCA
    GAGAAGCGGA AACGGAGCCC CACCGAAGGG CTTAGCAGTC CAGGCAGTAA GGAGAGCCCA
    TCGGGCCGAA TGGAGAGTGC CAGGGCGGCA GGGGTGCTTC TGGTACCCCG TATCCAATTG
    CCGCCACCCT CCTCTGGGAA ATCATCACGC CAGTTTGATA CCCGGAGTCT GAACGAGGAG
    AATTTTATTG CCTCCATTGA ATTGTGGAGC AAGCACCAGG ATAACAGAAA GCAAAAAGCT
    TTGGAAAAAG AACAGAGTGG TTTAAATAAA CGGAAATCAG GAGCGGATGG AGTAAAACAA
    CGTGTAGAAG GAGGAGACAC TGAGGAGAAG AAATCACAGA TCAGTGCAGA CAGTGGCCTC
    AGTGTGACAT CTGGCTCACA GAAGAGTGAT ACGGATTCCC TGGCGAGCTC TGAACCTCCT
    GCTCTGACCA GAAGCACCAG CCAGGACTCA GAGGCCAGCA CAGTGGTAAG TAACAGCTCA
    GGTGAGACGC TCGGTGCAGA CAGTGACCTG AGCAGCACTG CTGGAGATGG CCTGACAGGA
    AGACATGGTC AGCATCTCAA CCTGTCCCGT GGCACACTGT CTGACAGTGA GATTGAGACC
    AACCCTGCAA CCAGCTCAGT GTTTGGCAAG ACACACAAGC TGAAGCCAGG AGTGAAGGAG
    CCTGTTGGTG TTAATAAAGC AGCACCTGCT CCACCCGTTG AGGATGTTAG CATGAGGATC
    TATCTGTGTG AGGGGCTTCT GGGCCGGGAT AAAAGCTCAG TTTGGGACCA GTTGGAGGAT
    GCTGCAATGG AAACCTTTTC TCTGAGTAAA GAGCGCTCAA CTCTGTGGGA TCAGATGCAG
    TTCTGGGAGG ACGCCTTCCT GGATGCCGTA ATGCTGGAAC GGGAAGGAAT GGGAATGGAT
    CAGGGACCAC AAGAAATGAT TGACAGGTAT CTTTCTCTGG GAGATCATGA CCGCAAGAGA
    CTGGAGGATG ATGAGGACAG ACTGCTGGCC ACGCTTCTGC ACAATATGAT TGCTTACATG
    CTTATGATGA AGGTCGGTAA AAACGACATC AGGAAGAAAG TACGTCGTCT TATGGGCAAA
    TCCCACATTG GCCTCACTCA CAGTCAGGAG ATCAATGAAG TCCTGGATCG CCTCGCACAT
    TTAAGCGGTC GGGAGCTTTC TATCAGGCCG AGTGGCAGCA GGCATATTAA AAAACAGACA
    TTTGTTGTGC ATGCTGGGAC AGATACAACT GGTGACATAT TCTTCATGGA GGTGTGTGAT
    GATTGCATCG TCTTGCGCAG TAATATTGGC ACAGTCTATG AGCGCTGGTG GTATGAGAAG
    CTCATAAATA TGACTTACTG TCCCAAAACT AAAGTGTTGT GTCTGTGGAG GAGAAACGGC
    CAGGAGACGC AGCTAAACAA GTTTTACACT AAGAAGTGTC GGGAATTGTA CTACTGTGTT
    AAAGACAGCA TGGAGCGTGC TGCTGCTCGA CAACAAAGCA TCAAACCAGG TCCAGAGCTT
    GGAGGCGAGT TTCCGGTTCA GGACATGAAG ACAGGGGAGG GTGGTCTCCT CCAAGTCACA
    CTGGAAGGCA TCAACCTCAA ATTTATGCAC AGCCAGGAGC GGAAGGTTTT CATAGAGCTG
    AATCACATTA AAAAGTGCAA TACAGTGAAA GGAGTCTTTG TCCTGGAGGA GTTTGTTCCT
    GAAACTAAAG AAGTGGTGAT CCATAAGTAC AAGACTCCTA TGGCACATCA GATCTGTTAC
    TCGGTGCTCT GCCTCTTCTC CTACATGGCG GCGGTCAAGG GGAAAGAGTC AGAGGGCAAA
    CCCAAGATGC TGTCCCCTCG TCCACTAGCG AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301543.1
    CDS435..5510
    Translation

    Target ORF information:

    RefSeq Version XM_009303268.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303268.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGTG TCTGGTAAGG
    TTGACTCTGA TCACCCAAAA GCAGATCTTC GCCTCCGATT CTAAGGCTCT GGCTAGCATG
    AGTCTAAACA CACAGCCTAT TCTGAACCTG GAGAAGTTTC AGGATGGACA GGAGTTGTCT
    TTATTGCCCC CCGGTCGTGA CAAAGCTTCC CCGTCCTCCA CTGAGTTCAA CCCACTCATC
    TACGGCAATG ATGTGGACTC AGTTGATGTT GCCACAAGGG TGGCAATGGT GAGGTTCTTC
    AACTCTCCAA ACGTGCTGCA AGGGTTCCAG ATGCACACAC GCACTCTCCG TCTCTTCCCT
    CGTCCTGTGG TGGCCTTTCA GTCCACATCT TTCCTGGCTT CTAGGCCACG ACGAAATGGA
    TTCACAGAAA AGCTCTCCCA CACTCAGGCA GTGGAGTACT ATGGAGAATG GGCTCTCAAC
    CCCACCAACC TGGCTTTCCA AAGGATTCAT AACAATGTGT ATGACCCCTC TCTAATAGGC
    GATAAGCCTA AGTGGTATGC CCACCAGTTA CAGCCAGTAT TCTACCGCGT GTATGATGGA
    AACTCTCATT TAGCGGAGGC CTTGAGTGGA CCCCTGCAGG ATGAGACCAA TGATTCAGAC
    CCAACAGACG ACAGTGGCAG CGACAGTGAA GCATATGATG ACTCCAGCTC CTCCTACTCA
    TCTCTTGGAG ATTTTGTGAA TGAGATGATC AAAGGGGACA TACAGGGAGA CACACCAAAT
    GTTGATCCCC TGACCCATGC TGCACTGGGA GATGCAGATG AGGTTGAGAT CCATGACTTT
    CACGAGTATA AGGGGAACAG CGGTGAACCA GAGCCAGAAG GCCCCACAGA AGCAGCGGAC
    AGTCAGCCCT TGCGGTCCAG CTCTAGCACC ACAGCCAGTT CCAGCCCCAG TACAGTCATA
    CAGGGAGTCA ACCATGAGCA GAAGGAACCT GTTGAGGTGG AGCCCATAGC AAATGTGACT
    TACCCAAGCT CTGCCCCAGT ATTGGGTCCT CCTCCTTTTA CAAGACCCAC CCCTGATCCA
    ACCCTTGTTG ACCCTGCCAA TAAGAAGCGA GAATATGACA ACCCCTATTT TGAGCCCCAG
    TACGGTTTCC CTACAGAGGA AGATGCTGAG ACTGATGAGC AGGAGGAGAG TTACACACCA
    CGATTTAACC AGAATCTCAA TGGCAATAAG CCTTCTCGTC CACTGAGACC CAGCAGCCTG
    AAGCTTCCGG GTGAATCTGA CGGAGAGGGA GACTCCAGAA ATAGCTCTCC TAACTCAACC
    ATCTCCAACA ATAGCAGTGA TGGCTTTGGG GGACTCATGT CCTTTGCCAG TAACTTGTAT
    AAAAACCATG GCACCAGTTT CAGTCTGTCC AGCCTGGCTC TGCCTAATAA AGCGAGAGAG
    AAGAACACGC CGTTTCCCAG CCTCAAAGTA TTTGGGCTAA ATTCTCTAAT GGAGATTGTA
    ACAGAGATCG GCCCGGGGAG CGGAGAAGGT GCCCGAGCCC CCAGAGCACT GGTAGATCAG
    AAATCCTCTG TTATTAAGCA CAGCCCTACT GTGAAGAGAG AGTCTCCGTC TCCTCAAGGC
    AGAGCCAACA ATACCAGTGA GAACCAGCAG TTTCTAAAGG AGGTGGTCCA GAGCGTGCTG
    GAGGGGCAGG GTGTGGGCTG GCTTAACATG AAGAAGGTGC GTCGCCTGCT AGAGAATGAA
    CAGCTCAGAG TCTTTGTGCT CAGCAAACTC AACCGTGCGG TCCAGTCAGA GGAGGACGCC
    CAGCAGGAAG TTATAAGAGA TGTGGAGATA AATAGAAAGG TCTACAAAGG CATGCTGGAT
    ATTTTGAAGT GCACGGTGTC CAGTTTGGAG CATTCATACA CTAATGCTGG CTTAGGGGGC
    ATGGCCAGTG TCTTCAGTCT GCTAGAGATA GCACGCACCC ACTACCAAAC CAAAGATCCA
    GAGAAGCGGA AACGGAGCCC CACCGAAGGG CTTAGCAGTC CAGGCAGTAA GGAGAGCCCA
    TCGGGCCGAA TGGAGAGTGC CAGGGCGGCA GGGGTGCTTC TGGTACCCCG TATCCAATTG
    CCGCCACCCT CCTCTGGGAA ATCATCACGC CAGTTTGATA CCCGGAGTCT GAACGAGGAG
    AATTTTATTG CCTCCATTGA ATTGTGGAGC AAGCACCAGG ATAACAGAAA GCAAAAAGCT
    TTGGAAAAAG AACAGAGTGG TTTAAATAAA CGGAAATCAG GAGCGGATGG AGTAAAACAA
    CGTGTAGAAG GAGGAGACAC TGAGGAGAAG AAATCACAGA TCAGTGCAGA CAGTGGCCTC
    AGTGTGACAT CTGGCTCACA GAAGAGTGAT ACGGATTCCC TGGCGAGCTC TGAACCTCCT
    GCTCTGACCA GAAGCACCAG CCAGGACTCA GAGGCCAGCA CAGTGGTAAG TAACAGCTCA
    GGTGAGACGC TCGGTGCAGA CAGTGACCTG AGCAGCACTG CTGGAGATGG CCTGACAGGA
    AGACATGGTC AGCATCTCAA CCTGTCCCGT GGCACACTGT CTGACAGTGA GATTGAGACC
    AACCCTGCAA CCAGCTCAGT GTTTGGCAAG ACACACAAGC TGAAGCCAGG AGTGAAGGAG
    CCTGTTGGTG TTAATAAAGC AGCACCTGCT CCACCCGTTG AGGATGTTAG CATGAGGATC
    TATCTGTGTG AGGGGCTTCT GGGCCGGGAT AAAAGCTCAG TTTGGGACCA GTTGGAGGAT
    GCTGCAATGG AAACCTTTTC TCTGAGTAAA GAGCGCTCAA CTCTGTGGGA TCAGATGCAG
    TTCTGGGAGG ACGCCTTCCT GGATGCCGTA ATGCTGGAAC GGGAAGGAAT GGGAATGGAT
    CAGGGACCAC AAGAAATGAT TGACAGGTAT CTTTCTCTGG GAGATCATGA CCGCAAGAGA
    CTGGAGGATG ATGAGGACAG ACTGCTGGCC ACGCTTCTGC ACAATATGAT TGCTTACATG
    CTTATGATGA AGGTCGGTAA AAACGACATC AGGAAGAAAG TACGTCGTCT TATGGGCAAA
    TCCCACATTG GCCTCACTCA CAGTCAGGAG ATCAATGAAG TCCTGGATCG CCTCGCACAT
    TTAAGCGGTC GGGAGCTTTC TATCAGGCCG AGTGGCAGCA GGCATATTAA AAAACAGACA
    TTTGTTGTGC ATGCTGGGAC AGATACAACT GGTGACATAT TCTTCATGGA GGTGTGTGAT
    GATTGCATCG TCTTGCGCAG TAATATTGGC ACAGTCTATG AGCGCTGGTG GTATGAGAAG
    CTCATAAATA TGACTTACTG TCCCAAAACT AAAGTGTTGT GTCTGTGGAG GAGAAACGGC
    CAGGAGACGC AGCTAAACAA GTTTTACACT AAGAAGTGTC GGGAATTGTA CTACTGTGTT
    AAAGACAGCA TGGAGCGTGC TGCTGCTCGA CAACAAAGCA TCAAACCAGG TCCAGAGCTT
    GGAGGCGAGT TTCCGGTTCA GGACATGAAG ACAGGGGAGG GTGGTCTCCT CCAAGTCACA
    CTGGAAGGCA TCAACCTCAA ATTTATGCAC AGCCAGGAGC GGAAGGTTTT CATAGAGCTG
    AATCACATTA AAAAGTGCAA TACAGTGAAA GGAGTCTTTG TCCTGGAGGA GTTTGTTCCT
    GAAACTAAAG AAGTGGTGAT CCATAAGTAC AAGACTCCTA TGGCACATCA GATCTGTTAC
    TCGGTGCTCT GCCTCTTCTC CTACATGGCG GCGGTCAAGG GGAAAGAGTC AGAGGGCAAA
    CCCAAGATGC TGTCCCCTCG TCCACTAGCG AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48918
    Clone ID Related Accession (Same CDS sequence) XM_009303269.2
    Accession Version XM_009303269.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5058bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product MAP kinase-activating death domain protein isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303269.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGGCT
    CTGGCTAGCA TGAGTCTAAA CACACAGCCT ATTCTGAACC TGGAGAAGTT TCAGGATGGA
    CAGGAGTTGT CTTTATTGCC CCCCGGTCGT GACAAAGCTT CCCCGTCCTC CACTGAGTTC
    AACCCACTCA TCTACGGCAA TGATGTGGAC TCAGTTGATG TTGCCACAAG GGTGGCAATG
    GTGAGGTTCT TCAACTCTCC AAACGTGCTG CAAGGGTTCC AGATGCACAC ACGCACTCTC
    CGTCTCTTCC CTCGTCCTGT GGTGGCCTTT CAGTCCACAT CTTTCCTGGC TTCTAGGCCA
    CGACGAAATG GATTCACAGA AAAGCTCTCC CACACTCAGG CAGTGGAGTA CTATGGAGAA
    TGGGCTCTCA ACCCCACCAA CCTGGCTTTC CAAAGGATTC ATAACAATGT GTATGACCCC
    TCTCTAATAG GCGATAAGCC TAAGTGGTAT GCCCACCAGT TACAGCCAGT ATTCTACCGC
    GTGTATGATG GAAACTCTCA TTTAGCGGAG GCCTTGAGTG GACCCCTGCA GGATGAGACC
    AATGATTCAG ACCCAACAGA CGACAGTGGC AGCGACAGTG AAGCATATGA TGACTCCAGC
    TCCTCCTACT CATCTCTTGG AGATTTTGTG AATGAGATGA TCAAAGGGGA CATACAGGGA
    GACACACCAA ATGTTGATCC CCTGACCCAT GCTGCACTGG GAGATGCAGA TGAGGTTGAG
    ATCCATGACT TTCACGAGTA TAAGGGGAAC AGCGGTGAAC CAGAGCCAGA AGGCCCCACA
    GAAGCAGCGG ACAGTCAGCC CTTGCGGTCC AGCTCTAGCA CCACAGCCAG TTCCAGCCCC
    AGTACAGTCA TACAGGGAGT CAACCATGAG CAGAAGGAAC CTGTTGAGGT GGAGCCCATA
    GCAAATGTGA CTTACCCAAG CTCTGCCCCA GTATTGGGTC CTCCTCCTTT TACAAGACCC
    ACCCCTGATC CAACCCTTGT TGACCCTGCC AATAAGAAGC GAGAATATGA CAACCCCTAT
    TTTGAGCCCC AGTACGGTTT CCCTACAGAG GAAGATGCTG AGACTGATGA GCAGGAGGAG
    AGTTACACAC CACGATTTAA CCAGAATCTC AATGGCAATA AGCCTTCTCG TCCACTGAGA
    CCCAGCAGCC TGAAGCTTCC GGGTGAATCT GACGGAGAGG GAGACTCCAG AAATAGCTCT
    CCTAACTCAA CCATCTCCAA CAATAGCAGT GATGGCTTTG GGGGACTCAT GTCCTTTGCC
    AGTAACTTGT ATAAAAACCA TGGCACCAGT TTCAGTCTGT CCAGCCTGGC TCTGCCTAAT
    AAAGCGAGAG AGAAGAACAC GCCGTTTCCC AGCCTCAAAG ACGATGCTGA TGACAGTCCT
    GAAAGTCCTG TATTTGGGCT AAATTCTCTA ATGGAGATTG TAACAGAGAT CGGCCCGGGG
    AGCGGAGAAG GTGCCCGAGC CCCCAGAGCA CTGGTAGATC AGAAATCCTC TGTTATTAAG
    CACAGCCCTA CTGTGAAGAG AGAGTCTCCG TCTCCTCAAG GCAGAGCCAA CAATACCAGT
    GAGAACCAGC AGTTTCTAAA GGAGGTGGTC CAGAGCGTGC TGGAGGGGCA GGGTGTGGGC
    TGGCTTAACA TGAAGAAGGT GCGTCGCCTG CTAGAGAATG AACAGCTCAG AGTCTTTGTG
    CTCAGCAAAC TCAACCGTGC GGTCCAGTCA GAGGAGGACG CCCAGCAGGA AGTTATAAGA
    GATGTGGAGA TAAATAGAAA GGTCTACAAA GGCATGCTGG ATATTTTGAA GTGCACGGTG
    TCCAGTTTGG AGCATTCATA CACTAATGCT GGCTTAGGGG GCATGGCCAG TGTCTTCAGT
    CTGCTAGAGA TAGCACGCAC CCACTACCAA ACCAAAGATC CAGAGAAGCG GAAACGGAGC
    CCCACCGAAG GGCTTAGCAG TCCAGGCAGT AAGGAGAGCC CATCGGGCCG AATGGAGAGT
    GCCAGGGCGG CAGGGGTGCT TCTGGTACCC CGTATCCAAT TGCCGCCACC CTCCTCTGGG
    AAATCATCAC GCCAGTTTGA TACCCGGAGT CTGAACGAGG AGAATTTTAT TGCCTCCATT
    GAATTGTGGA GCAAGCACCA GGATAACAGA AAGCAAAAAG CTTTGGAAAA AGAACAGAGT
    GGTTTAAATA AACGGAAATC AGGAGCGGAT GGAGTAAAAC AACGTGTAGA AGGAGGAGAC
    ACTGAGGAGA AGAAATCACA GATCAGTGCA GACAGTGGCC TCAGTGTGAC ATCTGGCTCA
    CAGAAGAGTG ATACGGATTC CCTGGCGAGC TCTGAACCTC CTGCTCTGAC CAGAAGCACC
    AGCCAGGACT CAGAGGCCAG CACAGTGGTA AGTAACAGCT CAGGTGAGAC GCTCGGTGCA
    GACAGTGACC TGAGCAGCAC TGCTGGAGAT GGCCTGACAG GAAGACATGG TCAGCATCTC
    AACCTGTCCC GTGGCACACT GTCTGACAGT GAGATTGAGA CCAACCCTGC AACCAGCTCA
    GTGTTTGGCA AGACACACAA GCTGAAGCCA GGAGTGAAGG AGCCTGTTGG TGTTAATAAA
    GCAGCACCTG CTCCACCCGT TGAGGATGTT AGCATGAGGA TCTATCTGTG TGAGGGGCTT
    CTGGGCCGGG ATAAAAGCTC AGTTTGGGAC CAGTTGGAGG ATGCTGCAAT GGAAACCTTT
    TCTCTGAGTA AAGAGCGCTC AACTCTGTGG GATCAGATGC AGTTCTGGGA GGACGCCTTC
    CTGGATGCCG TAATGCTGGA ACGGGAAGGA ATGGGAATGG ATCAGGGACC ACAAGAAATG
    ATTGACAGGT ATCTTTCTCT GGGAGATCAT GACCGCAAGA GACTGGAGGA TGATGAGGAC
    AGACTGCTGG CCACGCTTCT GCACAATATG ATTGCTTACA TGCTTATGAT GAAGGTCGGT
    AAAAACGACA TCAGGAAGAA AGTACGTCGT CTTATGGGCA AATCCCACAT TGGCCTCACT
    CACAGTCAGG AGATCAATGA AGTCCTGGAT CGCCTCGCAC ATTTAAGCGG TCGGGAGCTT
    TCTATCAGGC CGAGTGGCAG CAGGCATATT AAAAAACAGA CATTTGTTGT GCATGCTGGG
    ACAGATACAA CTGGTGACAT ATTCTTCATG GAGGTGTGTG ATGATTGCAT CGTCTTGCGC
    AGTAATATTG GCACAGTCTA TGAGCGCTGG TGGTATGAGA AGCTCATAAA TATGACTTAC
    TGTCCCAAAA CTAAAGTGTT GTGTCTGTGG AGGAGAAACG GCCAGGAGAC GCAGCTAAAC
    AAGTTTTACA CTAAGAAGTG TCGGGAATTG TACTACTGTG TTAAAGACAG CATGGAGCGT
    GCTGCTGCTC GACAACAAAG CATCAAACCA GGTCCAGAGC TTGGAGGCGA GTTTCCGGTT
    CAGGACATGA AGACAGGGGA GGGTGGTCTC CTCCAAGTCA CACTGGAAGG CATCAACCTC
    AAATTTATGC ACAGCCAGGA GCGGAAGGTT TTCATAGAGC TGAATCACAT TAAAAAGTGC
    AATACAGTGA AAGGAGTCTT TGTCCTGGAG GAGTTTGTTC CTGAAACTAA AGAAGTGGTG
    ATCCATAAGT ACAAGACTCC TATGGCACAT CAGATCTGTT ACTCGGTGCT CTGCCTCTTC
    TCCTACATGG CGGCGGTCAA GGGGAAAGAG TCAGAGGGCA AACCCAAGAT GCTGTCCCCT
    CGTCCACTAG CGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301544.1
    CDS435..5492
    Translation

    Target ORF information:

    RefSeq Version XM_009303269.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303269.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGCT CTTGGAGGCT
    CTGGCTAGCA TGAGTCTAAA CACACAGCCT ATTCTGAACC TGGAGAAGTT TCAGGATGGA
    CAGGAGTTGT CTTTATTGCC CCCCGGTCGT GACAAAGCTT CCCCGTCCTC CACTGAGTTC
    AACCCACTCA TCTACGGCAA TGATGTGGAC TCAGTTGATG TTGCCACAAG GGTGGCAATG
    GTGAGGTTCT TCAACTCTCC AAACGTGCTG CAAGGGTTCC AGATGCACAC ACGCACTCTC
    CGTCTCTTCC CTCGTCCTGT GGTGGCCTTT CAGTCCACAT CTTTCCTGGC TTCTAGGCCA
    CGACGAAATG GATTCACAGA AAAGCTCTCC CACACTCAGG CAGTGGAGTA CTATGGAGAA
    TGGGCTCTCA ACCCCACCAA CCTGGCTTTC CAAAGGATTC ATAACAATGT GTATGACCCC
    TCTCTAATAG GCGATAAGCC TAAGTGGTAT GCCCACCAGT TACAGCCAGT ATTCTACCGC
    GTGTATGATG GAAACTCTCA TTTAGCGGAG GCCTTGAGTG GACCCCTGCA GGATGAGACC
    AATGATTCAG ACCCAACAGA CGACAGTGGC AGCGACAGTG AAGCATATGA TGACTCCAGC
    TCCTCCTACT CATCTCTTGG AGATTTTGTG AATGAGATGA TCAAAGGGGA CATACAGGGA
    GACACACCAA ATGTTGATCC CCTGACCCAT GCTGCACTGG GAGATGCAGA TGAGGTTGAG
    ATCCATGACT TTCACGAGTA TAAGGGGAAC AGCGGTGAAC CAGAGCCAGA AGGCCCCACA
    GAAGCAGCGG ACAGTCAGCC CTTGCGGTCC AGCTCTAGCA CCACAGCCAG TTCCAGCCCC
    AGTACAGTCA TACAGGGAGT CAACCATGAG CAGAAGGAAC CTGTTGAGGT GGAGCCCATA
    GCAAATGTGA CTTACCCAAG CTCTGCCCCA GTATTGGGTC CTCCTCCTTT TACAAGACCC
    ACCCCTGATC CAACCCTTGT TGACCCTGCC AATAAGAAGC GAGAATATGA CAACCCCTAT
    TTTGAGCCCC AGTACGGTTT CCCTACAGAG GAAGATGCTG AGACTGATGA GCAGGAGGAG
    AGTTACACAC CACGATTTAA CCAGAATCTC AATGGCAATA AGCCTTCTCG TCCACTGAGA
    CCCAGCAGCC TGAAGCTTCC GGGTGAATCT GACGGAGAGG GAGACTCCAG AAATAGCTCT
    CCTAACTCAA CCATCTCCAA CAATAGCAGT GATGGCTTTG GGGGACTCAT GTCCTTTGCC
    AGTAACTTGT ATAAAAACCA TGGCACCAGT TTCAGTCTGT CCAGCCTGGC TCTGCCTAAT
    AAAGCGAGAG AGAAGAACAC GCCGTTTCCC AGCCTCAAAG ACGATGCTGA TGACAGTCCT
    GAAAGTCCTG TATTTGGGCT AAATTCTCTA ATGGAGATTG TAACAGAGAT CGGCCCGGGG
    AGCGGAGAAG GTGCCCGAGC CCCCAGAGCA CTGGTAGATC AGAAATCCTC TGTTATTAAG
    CACAGCCCTA CTGTGAAGAG AGAGTCTCCG TCTCCTCAAG GCAGAGCCAA CAATACCAGT
    GAGAACCAGC AGTTTCTAAA GGAGGTGGTC CAGAGCGTGC TGGAGGGGCA GGGTGTGGGC
    TGGCTTAACA TGAAGAAGGT GCGTCGCCTG CTAGAGAATG AACAGCTCAG AGTCTTTGTG
    CTCAGCAAAC TCAACCGTGC GGTCCAGTCA GAGGAGGACG CCCAGCAGGA AGTTATAAGA
    GATGTGGAGA TAAATAGAAA GGTCTACAAA GGCATGCTGG ATATTTTGAA GTGCACGGTG
    TCCAGTTTGG AGCATTCATA CACTAATGCT GGCTTAGGGG GCATGGCCAG TGTCTTCAGT
    CTGCTAGAGA TAGCACGCAC CCACTACCAA ACCAAAGATC CAGAGAAGCG GAAACGGAGC
    CCCACCGAAG GGCTTAGCAG TCCAGGCAGT AAGGAGAGCC CATCGGGCCG AATGGAGAGT
    GCCAGGGCGG CAGGGGTGCT TCTGGTACCC CGTATCCAAT TGCCGCCACC CTCCTCTGGG
    AAATCATCAC GCCAGTTTGA TACCCGGAGT CTGAACGAGG AGAATTTTAT TGCCTCCATT
    GAATTGTGGA GCAAGCACCA GGATAACAGA AAGCAAAAAG CTTTGGAAAA AGAACAGAGT
    GGTTTAAATA AACGGAAATC AGGAGCGGAT GGAGTAAAAC AACGTGTAGA AGGAGGAGAC
    ACTGAGGAGA AGAAATCACA GATCAGTGCA GACAGTGGCC TCAGTGTGAC ATCTGGCTCA
    CAGAAGAGTG ATACGGATTC CCTGGCGAGC TCTGAACCTC CTGCTCTGAC CAGAAGCACC
    AGCCAGGACT CAGAGGCCAG CACAGTGGTA AGTAACAGCT CAGGTGAGAC GCTCGGTGCA
    GACAGTGACC TGAGCAGCAC TGCTGGAGAT GGCCTGACAG GAAGACATGG TCAGCATCTC
    AACCTGTCCC GTGGCACACT GTCTGACAGT GAGATTGAGA CCAACCCTGC AACCAGCTCA
    GTGTTTGGCA AGACACACAA GCTGAAGCCA GGAGTGAAGG AGCCTGTTGG TGTTAATAAA
    GCAGCACCTG CTCCACCCGT TGAGGATGTT AGCATGAGGA TCTATCTGTG TGAGGGGCTT
    CTGGGCCGGG ATAAAAGCTC AGTTTGGGAC CAGTTGGAGG ATGCTGCAAT GGAAACCTTT
    TCTCTGAGTA AAGAGCGCTC AACTCTGTGG GATCAGATGC AGTTCTGGGA GGACGCCTTC
    CTGGATGCCG TAATGCTGGA ACGGGAAGGA ATGGGAATGG ATCAGGGACC ACAAGAAATG
    ATTGACAGGT ATCTTTCTCT GGGAGATCAT GACCGCAAGA GACTGGAGGA TGATGAGGAC
    AGACTGCTGG CCACGCTTCT GCACAATATG ATTGCTTACA TGCTTATGAT GAAGGTCGGT
    AAAAACGACA TCAGGAAGAA AGTACGTCGT CTTATGGGCA AATCCCACAT TGGCCTCACT
    CACAGTCAGG AGATCAATGA AGTCCTGGAT CGCCTCGCAC ATTTAAGCGG TCGGGAGCTT
    TCTATCAGGC CGAGTGGCAG CAGGCATATT AAAAAACAGA CATTTGTTGT GCATGCTGGG
    ACAGATACAA CTGGTGACAT ATTCTTCATG GAGGTGTGTG ATGATTGCAT CGTCTTGCGC
    AGTAATATTG GCACAGTCTA TGAGCGCTGG TGGTATGAGA AGCTCATAAA TATGACTTAC
    TGTCCCAAAA CTAAAGTGTT GTGTCTGTGG AGGAGAAACG GCCAGGAGAC GCAGCTAAAC
    AAGTTTTACA CTAAGAAGTG TCGGGAATTG TACTACTGTG TTAAAGACAG CATGGAGCGT
    GCTGCTGCTC GACAACAAAG CATCAAACCA GGTCCAGAGC TTGGAGGCGA GTTTCCGGTT
    CAGGACATGA AGACAGGGGA GGGTGGTCTC CTCCAAGTCA CACTGGAAGG CATCAACCTC
    AAATTTATGC ACAGCCAGGA GCGGAAGGTT TTCATAGAGC TGAATCACAT TAAAAAGTGC
    AATACAGTGA AAGGAGTCTT TGTCCTGGAG GAGTTTGTTC CTGAAACTAA AGAAGTGGTG
    ATCCATAAGT ACAAGACTCC TATGGCACAT CAGATCTGTT ACTCGGTGCT CTGCCTCTTC
    TCCTACATGG CGGCGGTCAA GGGGAAAGAG TCAGAGGGCA AACCCAAGAT GCTGTCCCCT
    CGTCCACTAG CGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48919
    Clone ID Related Accession (Same CDS sequence) XM_009303270.2
    Accession Version XM_009303270.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5049bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product MAP kinase-activating death domain protein isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303270.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GACGATGCTG ATGACAGTCC TGAAAGTCCT
    GTATTTGGGC TAAATTCTCT AATGGAGATT GTAACAGAGA TCGGCCCGGG GAGCGGAGAA
    GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT CAGAAATCCT CTGTTATTAA GCACAGCCCT
    ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA GGCAGAGCCA ACAATACCAG TGAGAACCAG
    CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC
    ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT GAACAGCTCA GAGTCTTTGT GCTCAGCAAA
    CTCAACCGTG CGGTCCAGTC AGAGGAGGAC GCCCAGCAGG AAGTTATAAG AGATGTGGAG
    ATAAATAGAA AGGTCTACAA AGGCATGCTG GATATTTTGA AGTGCACGGT GTCCAGTTTG
    GAGCATTCAT ACACTAATGC TGGCTTAGGG GGCATGGCCA GTGTCTTCAG TCTGCTAGAG
    ATAGCACGCA CCCACTACCA AACCAAAGAT CCAGAGAAGC GGAAACGGAG CCCCACCGAA
    GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC CCATCGGGCC GAATGGAGAG TGCCAGGGCG
    GCAGGGGTGC TTCTGGTACC CCGTATCCAA TTGCCGCCAC CCTCCTCTGG GAAATCATCA
    CGCCAGTTTG ATACCCGGAG TCTGAACGAG GAGAATTTTA TTGCCTCCAT TGAATTGTGG
    AGCAAGCACC AGGATAACAG AAAGCAAAAA GCTTTGGAAA AAGAACAGAG TGGTTTAAAT
    AAACGGAAAT CAGGAGCGGA TGGAGTAAAA CAACGTGTAG AAGGAGGAGA CACTGAGGAG
    AAGAAATCAC AGATCAGTGC AGACAGTGGC CTCAGTGTGA CATCTGGCTC ACAGAAGAGT
    GATACGGATT CCCTGGCGAG CTCTGAACCT CCTGCTCTGA CCAGAAGCAC CAGCCAGGAC
    TCAGAGGCCA GCACAGTGGT AAGTAACAGC TCAGGTGAGA CGCTCGGTGC AGACAGTGAC
    CTGAGCAGCA CTGCTGGAGA TGGCCTGACA GGAAGACATG GTCAGCATCT CAACCTGTCC
    CGTGGCACAC TGTCTGACAG TGAGATTGAG ACCAACCCTG CAACCAGCTC AGTGTTTGGC
    AAGACACACA AGCTGAAGCC AGGAGTGAAG GAGCCTGTTG GTGTTAATAA AGCAGCACCT
    GCTCCACCCG TTGAGGATGT TAGCATGAGG ATCTATCTGT GTGAGGGGCT TCTGGGCCGG
    GATAAAAGCT CAGTTTGGGA CCAGTTGGAG GATGCTGCAA TGGAAACCTT TTCTCTGAGT
    AAAGAGCGCT CAACTCTGTG GGATCAGATG CAGTTCTGGG AGGACGCCTT CCTGGATGCC
    GTAATGCTGG AACGGGAAGG AATGGGAATG GATCAGGGAC CACAAGAAAT GATTGACAGG
    TATCTTTCTC TGGGAGATCA TGACCGCAAG AGACTGGAGG ATGATGAGGA CAGACTGCTG
    GCCACGCTTC TGCACAATAT GATTGCTTAC ATGCTTATGA TGAAGGTCGG TAAAAACGAC
    ATCAGGAAGA AAGTACGTCG TCTTATGGGC AAATCCCACA TTGGCCTCAC TCACAGTCAG
    GAGATCAATG AAGTCCTGGA TCGCCTCGCA CATTTAAGCG GTCGGGAGCT TTCTATCAGG
    CCGAGTGGCA GCAGGCATAT TAAAAAACAG ACATTTGTTG TGCATGCTGG GACAGATACA
    ACTGGTGACA TATTCTTCAT GGAGGTGTGT GATGATTGCA TCGTCTTGCG CAGTAATATT
    GGCACAGTCT ATGAGCGCTG GTGGTATGAG AAGCTCATAA ATATGACTTA CTGTCCCAAA
    ACTAAAGTGT TGTGTCTGTG GAGGAGAAAC GGCCAGGAGA CGCAGCTAAA CAAGTTTTAC
    ACTAAGAAGT GTCGGGAATT GTACTACTGT GTTAAAGACA GCATGGAGCG TGCTGCTGCT
    CGACAACAAA GCATCAAACC AGGTCCAGAG CTTGGAGGCG AGTTTCCGGT TCAGGACATG
    AAGACAGGGG AGGGTGGTCT CCTCCAAGTC ACACTGGAAG GCATCAACCT CAAATTTATG
    CACAGCCAGG AGCGGAAGGT TTTCATAGAG CTGAATCACA TTAAAAAGTG CAATACAGTG
    AAAGGAGTCT TTGTCCTGGA GGAGTTTGTT CCTGAAACTA AAGAAGTGGT GATCCATAAG
    TACAAGACTC CTATGGCACA TCAGATCTGT TACTCGGTGC TCTGCCTCTT CTCCTACATG
    GCGGCGGTCA AGGGGAAAGA GTCAGAGGGC AAACCCAAGA TGCTGTCCCC TCGTCCACTA
    GCGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301545.1
    CDS435..5483
    Translation

    Target ORF information:

    RefSeq Version XM_009303270.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303270.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GACGATGCTG ATGACAGTCC TGAAAGTCCT
    GTATTTGGGC TAAATTCTCT AATGGAGATT GTAACAGAGA TCGGCCCGGG GAGCGGAGAA
    GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT CAGAAATCCT CTGTTATTAA GCACAGCCCT
    ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA GGCAGAGCCA ACAATACCAG TGAGAACCAG
    CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC
    ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT GAACAGCTCA GAGTCTTTGT GCTCAGCAAA
    CTCAACCGTG CGGTCCAGTC AGAGGAGGAC GCCCAGCAGG AAGTTATAAG AGATGTGGAG
    ATAAATAGAA AGGTCTACAA AGGCATGCTG GATATTTTGA AGTGCACGGT GTCCAGTTTG
    GAGCATTCAT ACACTAATGC TGGCTTAGGG GGCATGGCCA GTGTCTTCAG TCTGCTAGAG
    ATAGCACGCA CCCACTACCA AACCAAAGAT CCAGAGAAGC GGAAACGGAG CCCCACCGAA
    GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC CCATCGGGCC GAATGGAGAG TGCCAGGGCG
    GCAGGGGTGC TTCTGGTACC CCGTATCCAA TTGCCGCCAC CCTCCTCTGG GAAATCATCA
    CGCCAGTTTG ATACCCGGAG TCTGAACGAG GAGAATTTTA TTGCCTCCAT TGAATTGTGG
    AGCAAGCACC AGGATAACAG AAAGCAAAAA GCTTTGGAAA AAGAACAGAG TGGTTTAAAT
    AAACGGAAAT CAGGAGCGGA TGGAGTAAAA CAACGTGTAG AAGGAGGAGA CACTGAGGAG
    AAGAAATCAC AGATCAGTGC AGACAGTGGC CTCAGTGTGA CATCTGGCTC ACAGAAGAGT
    GATACGGATT CCCTGGCGAG CTCTGAACCT CCTGCTCTGA CCAGAAGCAC CAGCCAGGAC
    TCAGAGGCCA GCACAGTGGT AAGTAACAGC TCAGGTGAGA CGCTCGGTGC AGACAGTGAC
    CTGAGCAGCA CTGCTGGAGA TGGCCTGACA GGAAGACATG GTCAGCATCT CAACCTGTCC
    CGTGGCACAC TGTCTGACAG TGAGATTGAG ACCAACCCTG CAACCAGCTC AGTGTTTGGC
    AAGACACACA AGCTGAAGCC AGGAGTGAAG GAGCCTGTTG GTGTTAATAA AGCAGCACCT
    GCTCCACCCG TTGAGGATGT TAGCATGAGG ATCTATCTGT GTGAGGGGCT TCTGGGCCGG
    GATAAAAGCT CAGTTTGGGA CCAGTTGGAG GATGCTGCAA TGGAAACCTT TTCTCTGAGT
    AAAGAGCGCT CAACTCTGTG GGATCAGATG CAGTTCTGGG AGGACGCCTT CCTGGATGCC
    GTAATGCTGG AACGGGAAGG AATGGGAATG GATCAGGGAC CACAAGAAAT GATTGACAGG
    TATCTTTCTC TGGGAGATCA TGACCGCAAG AGACTGGAGG ATGATGAGGA CAGACTGCTG
    GCCACGCTTC TGCACAATAT GATTGCTTAC ATGCTTATGA TGAAGGTCGG TAAAAACGAC
    ATCAGGAAGA AAGTACGTCG TCTTATGGGC AAATCCCACA TTGGCCTCAC TCACAGTCAG
    GAGATCAATG AAGTCCTGGA TCGCCTCGCA CATTTAAGCG GTCGGGAGCT TTCTATCAGG
    CCGAGTGGCA GCAGGCATAT TAAAAAACAG ACATTTGTTG TGCATGCTGG GACAGATACA
    ACTGGTGACA TATTCTTCAT GGAGGTGTGT GATGATTGCA TCGTCTTGCG CAGTAATATT
    GGCACAGTCT ATGAGCGCTG GTGGTATGAG AAGCTCATAA ATATGACTTA CTGTCCCAAA
    ACTAAAGTGT TGTGTCTGTG GAGGAGAAAC GGCCAGGAGA CGCAGCTAAA CAAGTTTTAC
    ACTAAGAAGT GTCGGGAATT GTACTACTGT GTTAAAGACA GCATGGAGCG TGCTGCTGCT
    CGACAACAAA GCATCAAACC AGGTCCAGAG CTTGGAGGCG AGTTTCCGGT TCAGGACATG
    AAGACAGGGG AGGGTGGTCT CCTCCAAGTC ACACTGGAAG GCATCAACCT CAAATTTATG
    CACAGCCAGG AGCGGAAGGT TTTCATAGAG CTGAATCACA TTAAAAAGTG CAATACAGTG
    AAAGGAGTCT TTGTCCTGGA GGAGTTTGTT CCTGAAACTA AAGAAGTGGT GATCCATAAG
    TACAAGACTC CTATGGCACA TCAGATCTGT TACTCGGTGC TCTGCCTCTT CTCCTACATG
    GCGGCGGTCA AGGGGAAAGA GTCAGAGGGC AAACCCAAGA TGCTGTCCCC TCGTCCACTA
    GCGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48920
    Clone ID Related Accession (Same CDS sequence) XM_009303271.2
    Accession Version XM_009303271.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5046bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product MAP kinase-activating death domain protein isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303271.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGTG TCTGGTAAGG
    TTGACTCTGA TCACCCAAAA GCAGATCTTC GCCTCCGATT CTAAGGCTCT GGCTAGCATG
    AGTCTAAACA CACAGCCTAT TCTGAACCTG GAGAAGTTTC AGGATGGACA GGAGTTGTCT
    TTATTGCCCC CCGGTCGTGA CAAAGCTTCC CCGTCCTCCA CTGAGTTCAA CCCACTCATC
    TACGGCAATG ATGTGGACTC AGTTGATGTT GCCACAAGGG TGGCAATGGT GAGGTTCTTC
    AACTCTCCAA ACGTGCTGCA AGGGTTCCAG ATGCACACAC GCACTCTCCG TCTCTTCCCT
    CGTCCTGTGG TGGCCTTTCA GTCCACATCT TTCCTGGCTT CTAGGCCACG ACGAAATGGA
    TTCACAGAAA AGCTCTCCCA CACTCAGGCA GTGGAGTACT ATGGAGAATG GGCTCTCAAC
    CCCACCAACC TGGCTTTCCA AAGGATTCAT AACAATGTGT ATGACCCCTC TCTAATAGGC
    GATAAGCCTA AGTGGTATGC CCACCAGTTA CAGCCAGTAT TCTACCGCGT GTATGATGGA
    AACTCTCATT TAGCGGAGGC CTTGAGTGGA CCCCTGCAGG ATGAGACCAA TGATTCAGAC
    CCAACAGACG ACAGTGGCAG CGACAGTGAA GCATATGATG ACTCCAGCTC CTCCTACTCA
    TCTCTTGGAG ATTTTGTGAA TGAGATGATC AAAGGGGACA TACAGGGAGA CACACCAAAT
    GTTGATCCCC TGACCCATGC TGCACTGGGA GATGCAGATG AGGTTGAGAT CCATGACTTT
    CACGAGTATA AGGGGAACAG CGGTGAACCA GAGCCAGAAG GCCCCACAGA AGCAGCGGAC
    AGTCAGCCCT TGCGGTCCAG CTCTAGCACC ACAGCCAGTT CCAGCCCCAG TACAGTCATA
    CAGGGAGTCA ACCATGAGCA GAAGGAACCT GTTGAGGTGG AGCCCATAGC AAATGTGACT
    TACCCAAGCT CTGCCCCAGT ATTGGGTCCT CCTCCTTTTA CAAGACCCAC CCCTGATCCA
    ACCCTTGTTG ACCCTGCCAA TAAGAAGCGA GAATATGACA ACCCCTATTT TGAGCCCCAG
    TACGGTTTCC CTACAGAGGA AGATGCTGAG ACTGATGAGC AGGAGGAGAG TTACACACCA
    CGATTTAACC AGAATCTCAA TGGCAATAAG CCTTCTCGTC CACTGAGACC CAGCAGCCTG
    AAGCTTCCGG GTGAATCTGA CGGAGAGGGA GACTCCAGAA ATAGCTCTCC TAACTCAACC
    ATCTCCAACA ATAGCAGTGA TGGCTTTGGG GGACTCATGT CCTTTGCCAG TAACTTGTAT
    AAAAACCATG GCACCAGTTT CAGTCTGTCC AGCCTGGCTC TGCCTAATAA AGCGAGAGAG
    AAGAACACGC CGTTTCCCAG CCTCAAAGAC GATGCTGATG ACAGTCCTGA AAGTCCTGGT
    GCCCGAGCCC CCAGAGCACT GGTAGATCAG AAATCCTCTG TTATTAAGCA CAGCCCTACT
    GTGAAGAGAG AGTCTCCGTC TCCTCAAGGC AGAGCCAACA ATACCAGTGA GAACCAGCAG
    TTTCTAAAGG AGGTGGTCCA GAGCGTGCTG GAGGGGCAGG GTGTGGGCTG GCTTAACATG
    AAGAAGGTGC GTCGCCTGCT AGAGAATGAA CAGCTCAGAG TCTTTGTGCT CAGCAAACTC
    AACCGTGCGG TCCAGTCAGA GGAGGACGCC CAGCAGGAAG TTATAAGAGA TGTGGAGATA
    AATAGAAAGG TCTACAAAGG CATGCTGGAT ATTTTGAAGT GCACGGTGTC CAGTTTGGAG
    CATTCATACA CTAATGCTGG CTTAGGGGGC ATGGCCAGTG TCTTCAGTCT GCTAGAGATA
    GCACGCACCC ACTACCAAAC CAAAGATCCA GAGAAGCGGA AACGGAGCCC CACCGAAGGG
    CTTAGCAGTC CAGGCAGTAA GGAGAGCCCA TCGGGCCGAA TGGAGAGTGC CAGGGCGGCA
    GGGGTGCTTC TGGTACCCCG TATCCAATTG CCGCCACCCT CCTCTGGGAA ATCATCACGC
    CAGTTTGATA CCCGGAGTCT GAACGAGGAG AATTTTATTG CCTCCATTGA ATTGTGGAGC
    AAGCACCAGG ATAACAGAAA GCAAAAAGCT TTGGAAAAAG AACAGAGTGG TTTAAATAAA
    CGGAAATCAG GAGCGGATGG AGTAAAACAA CGTGTAGAAG GAGGAGACAC TGAGGAGAAG
    AAATCACAGA TCAGTGCAGA CAGTGGCCTC AGTGTGACAT CTGGCTCACA GAAGAGTGAT
    ACGGATTCCC TGGCGAGCTC TGAACCTCCT GCTCTGACCA GAAGCACCAG CCAGGACTCA
    GAGGCCAGCA CAGTGGTAAG TAACAGCTCA GGTGAGACGC TCGGTGCAGA CAGTGACCTG
    AGCAGCACTG CTGGAGATGG CCTGACAGGA AGACATGGTC AGCATCTCAA CCTGTCCCGT
    GGCACACTGT CTGACAGTGA GATTGAGACC AACCCTGCAA CCAGCTCAGT GTTTGGCAAG
    ACACACAAGC TGAAGCCAGG AGTGAAGGAG CCTGTTGGTG TTAATAAAGC AGCACCTGCT
    CCACCCGTTG AGGATGTTAG CATGAGGATC TATCTGTGTG AGGGGCTTCT GGGCCGGGAT
    AAAAGCTCAG TTTGGGACCA GTTGGAGGAT GCTGCAATGG AAACCTTTTC TCTGAGTAAA
    GAGCGCTCAA CTCTGTGGGA TCAGATGCAG TTCTGGGAGG ACGCCTTCCT GGATGCCGTA
    ATGCTGGAAC GGGAAGGAAT GGGAATGGAT CAGGGACCAC AAGAAATGAT TGACAGGTAT
    CTTTCTCTGG GAGATCATGA CCGCAAGAGA CTGGAGGATG ATGAGGACAG ACTGCTGGCC
    ACGCTTCTGC ACAATATGAT TGCTTACATG CTTATGATGA AGGTCGGTAA AAACGACATC
    AGGAAGAAAG TACGTCGTCT TATGGGCAAA TCCCACATTG GCCTCACTCA CAGTCAGGAG
    ATCAATGAAG TCCTGGATCG CCTCGCACAT TTAAGCGGTC GGGAGCTTTC TATCAGGCCG
    AGTGGCAGCA GGCATATTAA AAAACAGACA TTTGTTGTGC ATGCTGGGAC AGATACAACT
    GGTGACATAT TCTTCATGGA GGTGTGTGAT GATTGCATCG TCTTGCGCAG TAATATTGGC
    ACAGTCTATG AGCGCTGGTG GTATGAGAAG CTCATAAATA TGACTTACTG TCCCAAAACT
    AAAGTGTTGT GTCTGTGGAG GAGAAACGGC CAGGAGACGC AGCTAAACAA GTTTTACACT
    AAGAAGTGTC GGGAATTGTA CTACTGTGTT AAAGACAGCA TGGAGCGTGC TGCTGCTCGA
    CAACAAAGCA TCAAACCAGG TCCAGAGCTT GGAGGCGAGT TTCCGGTTCA GGACATGAAG
    ACAGGGGAGG GTGGTCTCCT CCAAGTCACA CTGGAAGGCA TCAACCTCAA ATTTATGCAC
    AGCCAGGAGC GGAAGGTTTT CATAGAGCTG AATCACATTA AAAAGTGCAA TACAGTGAAA
    GGAGTCTTTG TCCTGGAGGA GTTTGTTCCT GAAACTAAAG AAGTGGTGAT CCATAAGTAC
    AAGACTCCTA TGGCACATCA GATCTGTTAC TCGGTGCTCT GCCTCTTCTC CTACATGGCG
    GCGGTCAAGG GGAAAGAGTC AGAGGGCAAA CCCAAGATGC TGTCCCCTCG TCCACTAGCG
    AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301546.1
    CDS435..5480
    Translation

    Target ORF information:

    RefSeq Version XM_009303271.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303271.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGTG TCTGGTAAGG
    TTGACTCTGA TCACCCAAAA GCAGATCTTC GCCTCCGATT CTAAGGCTCT GGCTAGCATG
    AGTCTAAACA CACAGCCTAT TCTGAACCTG GAGAAGTTTC AGGATGGACA GGAGTTGTCT
    TTATTGCCCC CCGGTCGTGA CAAAGCTTCC CCGTCCTCCA CTGAGTTCAA CCCACTCATC
    TACGGCAATG ATGTGGACTC AGTTGATGTT GCCACAAGGG TGGCAATGGT GAGGTTCTTC
    AACTCTCCAA ACGTGCTGCA AGGGTTCCAG ATGCACACAC GCACTCTCCG TCTCTTCCCT
    CGTCCTGTGG TGGCCTTTCA GTCCACATCT TTCCTGGCTT CTAGGCCACG ACGAAATGGA
    TTCACAGAAA AGCTCTCCCA CACTCAGGCA GTGGAGTACT ATGGAGAATG GGCTCTCAAC
    CCCACCAACC TGGCTTTCCA AAGGATTCAT AACAATGTGT ATGACCCCTC TCTAATAGGC
    GATAAGCCTA AGTGGTATGC CCACCAGTTA CAGCCAGTAT TCTACCGCGT GTATGATGGA
    AACTCTCATT TAGCGGAGGC CTTGAGTGGA CCCCTGCAGG ATGAGACCAA TGATTCAGAC
    CCAACAGACG ACAGTGGCAG CGACAGTGAA GCATATGATG ACTCCAGCTC CTCCTACTCA
    TCTCTTGGAG ATTTTGTGAA TGAGATGATC AAAGGGGACA TACAGGGAGA CACACCAAAT
    GTTGATCCCC TGACCCATGC TGCACTGGGA GATGCAGATG AGGTTGAGAT CCATGACTTT
    CACGAGTATA AGGGGAACAG CGGTGAACCA GAGCCAGAAG GCCCCACAGA AGCAGCGGAC
    AGTCAGCCCT TGCGGTCCAG CTCTAGCACC ACAGCCAGTT CCAGCCCCAG TACAGTCATA
    CAGGGAGTCA ACCATGAGCA GAAGGAACCT GTTGAGGTGG AGCCCATAGC AAATGTGACT
    TACCCAAGCT CTGCCCCAGT ATTGGGTCCT CCTCCTTTTA CAAGACCCAC CCCTGATCCA
    ACCCTTGTTG ACCCTGCCAA TAAGAAGCGA GAATATGACA ACCCCTATTT TGAGCCCCAG
    TACGGTTTCC CTACAGAGGA AGATGCTGAG ACTGATGAGC AGGAGGAGAG TTACACACCA
    CGATTTAACC AGAATCTCAA TGGCAATAAG CCTTCTCGTC CACTGAGACC CAGCAGCCTG
    AAGCTTCCGG GTGAATCTGA CGGAGAGGGA GACTCCAGAA ATAGCTCTCC TAACTCAACC
    ATCTCCAACA ATAGCAGTGA TGGCTTTGGG GGACTCATGT CCTTTGCCAG TAACTTGTAT
    AAAAACCATG GCACCAGTTT CAGTCTGTCC AGCCTGGCTC TGCCTAATAA AGCGAGAGAG
    AAGAACACGC CGTTTCCCAG CCTCAAAGAC GATGCTGATG ACAGTCCTGA AAGTCCTGGT
    GCCCGAGCCC CCAGAGCACT GGTAGATCAG AAATCCTCTG TTATTAAGCA CAGCCCTACT
    GTGAAGAGAG AGTCTCCGTC TCCTCAAGGC AGAGCCAACA ATACCAGTGA GAACCAGCAG
    TTTCTAAAGG AGGTGGTCCA GAGCGTGCTG GAGGGGCAGG GTGTGGGCTG GCTTAACATG
    AAGAAGGTGC GTCGCCTGCT AGAGAATGAA CAGCTCAGAG TCTTTGTGCT CAGCAAACTC
    AACCGTGCGG TCCAGTCAGA GGAGGACGCC CAGCAGGAAG TTATAAGAGA TGTGGAGATA
    AATAGAAAGG TCTACAAAGG CATGCTGGAT ATTTTGAAGT GCACGGTGTC CAGTTTGGAG
    CATTCATACA CTAATGCTGG CTTAGGGGGC ATGGCCAGTG TCTTCAGTCT GCTAGAGATA
    GCACGCACCC ACTACCAAAC CAAAGATCCA GAGAAGCGGA AACGGAGCCC CACCGAAGGG
    CTTAGCAGTC CAGGCAGTAA GGAGAGCCCA TCGGGCCGAA TGGAGAGTGC CAGGGCGGCA
    GGGGTGCTTC TGGTACCCCG TATCCAATTG CCGCCACCCT CCTCTGGGAA ATCATCACGC
    CAGTTTGATA CCCGGAGTCT GAACGAGGAG AATTTTATTG CCTCCATTGA ATTGTGGAGC
    AAGCACCAGG ATAACAGAAA GCAAAAAGCT TTGGAAAAAG AACAGAGTGG TTTAAATAAA
    CGGAAATCAG GAGCGGATGG AGTAAAACAA CGTGTAGAAG GAGGAGACAC TGAGGAGAAG
    AAATCACAGA TCAGTGCAGA CAGTGGCCTC AGTGTGACAT CTGGCTCACA GAAGAGTGAT
    ACGGATTCCC TGGCGAGCTC TGAACCTCCT GCTCTGACCA GAAGCACCAG CCAGGACTCA
    GAGGCCAGCA CAGTGGTAAG TAACAGCTCA GGTGAGACGC TCGGTGCAGA CAGTGACCTG
    AGCAGCACTG CTGGAGATGG CCTGACAGGA AGACATGGTC AGCATCTCAA CCTGTCCCGT
    GGCACACTGT CTGACAGTGA GATTGAGACC AACCCTGCAA CCAGCTCAGT GTTTGGCAAG
    ACACACAAGC TGAAGCCAGG AGTGAAGGAG CCTGTTGGTG TTAATAAAGC AGCACCTGCT
    CCACCCGTTG AGGATGTTAG CATGAGGATC TATCTGTGTG AGGGGCTTCT GGGCCGGGAT
    AAAAGCTCAG TTTGGGACCA GTTGGAGGAT GCTGCAATGG AAACCTTTTC TCTGAGTAAA
    GAGCGCTCAA CTCTGTGGGA TCAGATGCAG TTCTGGGAGG ACGCCTTCCT GGATGCCGTA
    ATGCTGGAAC GGGAAGGAAT GGGAATGGAT CAGGGACCAC AAGAAATGAT TGACAGGTAT
    CTTTCTCTGG GAGATCATGA CCGCAAGAGA CTGGAGGATG ATGAGGACAG ACTGCTGGCC
    ACGCTTCTGC ACAATATGAT TGCTTACATG CTTATGATGA AGGTCGGTAA AAACGACATC
    AGGAAGAAAG TACGTCGTCT TATGGGCAAA TCCCACATTG GCCTCACTCA CAGTCAGGAG
    ATCAATGAAG TCCTGGATCG CCTCGCACAT TTAAGCGGTC GGGAGCTTTC TATCAGGCCG
    AGTGGCAGCA GGCATATTAA AAAACAGACA TTTGTTGTGC ATGCTGGGAC AGATACAACT
    GGTGACATAT TCTTCATGGA GGTGTGTGAT GATTGCATCG TCTTGCGCAG TAATATTGGC
    ACAGTCTATG AGCGCTGGTG GTATGAGAAG CTCATAAATA TGACTTACTG TCCCAAAACT
    AAAGTGTTGT GTCTGTGGAG GAGAAACGGC CAGGAGACGC AGCTAAACAA GTTTTACACT
    AAGAAGTGTC GGGAATTGTA CTACTGTGTT AAAGACAGCA TGGAGCGTGC TGCTGCTCGA
    CAACAAAGCA TCAAACCAGG TCCAGAGCTT GGAGGCGAGT TTCCGGTTCA GGACATGAAG
    ACAGGGGAGG GTGGTCTCCT CCAAGTCACA CTGGAAGGCA TCAACCTCAA ATTTATGCAC
    AGCCAGGAGC GGAAGGTTTT CATAGAGCTG AATCACATTA AAAAGTGCAA TACAGTGAAA
    GGAGTCTTTG TCCTGGAGGA GTTTGTTCCT GAAACTAAAG AAGTGGTGAT CCATAAGTAC
    AAGACTCCTA TGGCACATCA GATCTGTTAC TCGGTGCTCT GCCTCTTCTC CTACATGGCG
    GCGGTCAAGG GGAAAGAGTC AGAGGGCAAA CCCAAGATGC TGTCCCCTCG TCCACTAGCG
    AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48921
    Clone ID Related Accession (Same CDS sequence) XM_009303272.2
    Accession Version XM_009303272.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5043bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product MAP kinase-activating death domain protein isoform X9
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303272.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGTG TCTGGTAAGG
    TTGACTCTGA TCACCCAAAA GCAGATCTTC GCCTCCGATT CTAAGGCTCT GGCTAGCATG
    AGTCTAAACA CACAGCCTAT TCTGAACCTG GAGAAGTTTC AGGATGGACA GGAGTTGTCT
    TTATTGCCCC CCGGTCGTGA CAAAGCTTCC CCGTCCTCCA CTGAGTTCAA CCCACTCATC
    TACGGCAATG ATGTGGACTC AGTTGATGTT GCCACAAGGG TGGCAATGGT GAGGTTCTTC
    AACTCTCCAA ACGTGCTGCA AGGGTTCCAG ATGCACACAC GCACTCTCCG TCTCTTCCCT
    CGTCCTGTGG TGGCCTTTCA GTCCACATCT TTCCTGGCTT CTAGGCCACG ACGAAATGGA
    TTCACAGAAA AGCTCTCCCA CACTCAGGCA GTGGAGTACT ATGGAGAATG GGCTCTCAAC
    CCCACCAACC TGGCTTTCCA AAGGATTCAT AACAATGTGT ATGACCCCTC TCTAATAGGC
    GATAAGCCTA AGTGGTATGC CCACCAGTTA CAGCCAGTAT TCTACCGCGT GTATGATGGA
    AACTCTCATT TAGCGGAGGC CTTGAGTGGA CCCCTGCAGG ATGAGACCAA TGATTCAGAC
    CCAACAGACG ACAGTGGCAG CGACAGTGAA GCATATGATG ACTCCAGCTC CTCCTACTCA
    TCTCTTGGAG ATTTTGTGAA TGAGATGATC AAAGGGGACA TACAGGGAGA CACACCAAAT
    GTTGATCCCC TGACCCATGC TGCACTGGGA GATGCAGATG AGGTTGAGAT CCATGACTTT
    CACGAGTATA AGGGGAACAG CGGTGAACCA GAGCCAGAAG GCCCCACAGA AGCAGCGGAC
    AGTCAGCCCT TGCGGTCCAG CTCTAGCACC ACAGCCAGTT CCAGCCCCAG TACAGTCATA
    CAGGGAGTCA ACCATGAGCA GAAGGAACCT GTTGAGGTGG AGCCCATAGC AAATGTGACT
    TACCCAAGCT CTGCCCCAGT ATTGGGTCCT CCTCCTTTTA CAAGACCCAC CCCTGATCCA
    ACCCTTGTTG ACCCTGCCAA TAAGAAGCGA GAATATGACA ACCCCTATTT TGAGCCCCAG
    TACGGTTTCC CTACAGAGGA AGATGCTGAG ACTGATGAGC AGGAGGAGAG TTACACACCA
    CGATTTAACC AGAATCTCAA TGGCAATAAG CCTTCTCGTC CACTGAGACC CAGCAGCCTG
    AAGCTTCCGG GTGAATCTGA CGGAGAGGGA GACTCCAGAA ATAGCTCTCC TAACTCAACC
    ATCTCCAACA ATAGCAGTGA TGGCTTTGGG GGACTCATGT CCTTTGCCAG TAACTTGTAT
    AAAAACCATG GCACCAGTTT CAGTCTGTCC AGCCTGGCTC TGCCTAATAA AGCGAGAGAG
    AAGAACACGC CGTTTCCCAG CCTCAAAGAC GATGCTGATG ACAGTCCTGA AAGTCCTGTA
    TTTGGGCTAA ATTCTCTAAT GGAGATTGTA ACAGAGATCG GCCCGGGGAG CGGAGAAGGT
    GCCCGAGCCC CCAGAGCACT GGTAGATCAG AAATCCTCTG TTATTAAGCA CAGCCCTACT
    GTGAAGAGAG AGTCTCCGTC TCCTCAAGGC AGAGCCAACA ATACCAGTGA GAACCAGCAG
    TTTCTAAAGG AGGTGGTCCA GAGCGTGCTG GAGGGGCAGG GTGTGGGCTG GCTTAACATG
    AAGAAGGTGC GTCGCCTGCT AGAGAATGAA CAGCTCAGAG TCTTTGTGCT CAGCAAACTC
    AACCGTGCGG TCCAGTCAGA GGAGGACGCC CAGCAGGAAG TTATAAGAGA TGTGGAGATA
    AATAGAAAGG TCTACAAAGG CATGCTGGAT ATTTTGAAGT GCACGGTGTC CAGTTTGGAG
    CATTCATACA CTAATGCTGG CTTAGGGGGC ATGGCCAGTG TCTTCAGTCT GCTAGAGATA
    GCACGCACCC ACTACCAAAC CAAAGATCCA GAGAAGCGGA AACGGAGCCC CACCGAAGGG
    CTTAGCAGTC CAGGCAGTAA GGAGAGCCCA TCGGGCCGAA TGGAGAGTGC CAGGGCGGCA
    GGGGTGCTTC TGGTACCCCG TATCCAATTG CCGCCACCCT CCTCTGGGAA ATCATCACGC
    CAGTTTGATA CCCGGAGTCT GAACGAGGAG AATTTTATTG CCTCCATTGA ATTGTGGAGC
    AAGCACCAGG ATAACAGAAA GCAAAAAGCT TTGGAAAAAG AACAGAGTGG TTTAAATAAA
    CGGAAATCAG GAGCGGATGG AGTAAAACAA CGTGTAGAAG GAGGAGACAC TGAGGAGAAG
    AAATCACAGA TCAGTGCAGA CAGTGGCCTC AGTGTGACAT CTGGCTCACA GAAGAGTGAT
    ACGGATTCCC TGGCGAGCTC TGAACCTCCT GCTCTGACCA GAAGCACCAG CCAGGACTCA
    GAGGCCAGCA CAGTGGTAAG TAACAGCTCA GGTGAGACGC TCGGTGCAGA CAGTGACCTG
    AGCAGCACTG CTGGAGATGG CCTGACAGGA AGACATGGTC AGCATCTCAA CCTGTCCCGT
    GGCACACTGT CTGACAGTGA GATTGAGACC AACCCTGCAA CCAGCTCAGT GTTTGGCAAG
    ACACACAAGC TGAAGCCAGG AGTGAAGGAG CCTGTTGGTG TTAATAAAGC AGCACCTGCT
    CCACCCGTTG AGGATGTTAG CATGAGGATC TATCTGTGTG AGGGGCTTCT GGGTAAAGAG
    CGCTCAACTC TGTGGGATCA GATGCAGTTC TGGGAGGACG CCTTCCTGGA TGCCGTAATG
    CTGGAACGGG AAGGAATGGG AATGGATCAG GGACCACAAG AAATGATTGA CAGGTATCTT
    TCTCTGGGAG ATCATGACCG CAAGAGACTG GAGGATGATG AGGACAGACT GCTGGCCACG
    CTTCTGCACA ATATGATTGC TTACATGCTT ATGATGAAGG TCGGTAAAAA CGACATCAGG
    AAGAAAGTAC GTCGTCTTAT GGGCAAATCC CACATTGGCC TCACTCACAG TCAGGAGATC
    AATGAAGTCC TGGATCGCCT CGCACATTTA AGCGGTCGGG AGCTTTCTAT CAGGCCGAGT
    GGCAGCAGGC ATATTAAAAA ACAGACATTT GTTGTGCATG CTGGGACAGA TACAACTGGT
    GACATATTCT TCATGGAGGT GTGTGATGAT TGCATCGTCT TGCGCAGTAA TATTGGCACA
    GTCTATGAGC GCTGGTGGTA TGAGAAGCTC ATAAATATGA CTTACTGTCC CAAAACTAAA
    GTGTTGTGTC TGTGGAGGAG AAACGGCCAG GAGACGCAGC TAAACAAGTT TTACACTAAG
    AAGTGTCGGG AATTGTACTA CTGTGTTAAA GACAGCATGG AGCGTGCTGC TGCTCGACAA
    CAAAGCATCA AACCAGGTCC AGAGCTTGGA GGCGAGTTTC CGGTTCAGGA CATGAAGACA
    GGGGAGGGTG GTCTCCTCCA AGTCACACTG GAAGGCATCA ACCTCAAATT TATGCACAGC
    CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT CACATTAAAA AGTGCAATAC AGTGAAAGGA
    GTCTTTGTCC TGGAGGAGTT TGTTCCTGAA ACTAAAGAAG TGGTGATCCA TAAGTACAAG
    ACTCCTATGG CACATCAGAT CTGTTACTCG GTGCTCTGCC TCTTCTCCTA CATGGCGGCG
    GTCAAGGGGA AAGAGTCAGA GGGCAAACCC AAGATGCTGT CCCCTCGTCC ACTAGCGAGC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301547.1
    CDS435..5477
    Translation

    Target ORF information:

    RefSeq Version XM_009303272.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303272.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGTG TCTGGTAAGG
    TTGACTCTGA TCACCCAAAA GCAGATCTTC GCCTCCGATT CTAAGGCTCT GGCTAGCATG
    AGTCTAAACA CACAGCCTAT TCTGAACCTG GAGAAGTTTC AGGATGGACA GGAGTTGTCT
    TTATTGCCCC CCGGTCGTGA CAAAGCTTCC CCGTCCTCCA CTGAGTTCAA CCCACTCATC
    TACGGCAATG ATGTGGACTC AGTTGATGTT GCCACAAGGG TGGCAATGGT GAGGTTCTTC
    AACTCTCCAA ACGTGCTGCA AGGGTTCCAG ATGCACACAC GCACTCTCCG TCTCTTCCCT
    CGTCCTGTGG TGGCCTTTCA GTCCACATCT TTCCTGGCTT CTAGGCCACG ACGAAATGGA
    TTCACAGAAA AGCTCTCCCA CACTCAGGCA GTGGAGTACT ATGGAGAATG GGCTCTCAAC
    CCCACCAACC TGGCTTTCCA AAGGATTCAT AACAATGTGT ATGACCCCTC TCTAATAGGC
    GATAAGCCTA AGTGGTATGC CCACCAGTTA CAGCCAGTAT TCTACCGCGT GTATGATGGA
    AACTCTCATT TAGCGGAGGC CTTGAGTGGA CCCCTGCAGG ATGAGACCAA TGATTCAGAC
    CCAACAGACG ACAGTGGCAG CGACAGTGAA GCATATGATG ACTCCAGCTC CTCCTACTCA
    TCTCTTGGAG ATTTTGTGAA TGAGATGATC AAAGGGGACA TACAGGGAGA CACACCAAAT
    GTTGATCCCC TGACCCATGC TGCACTGGGA GATGCAGATG AGGTTGAGAT CCATGACTTT
    CACGAGTATA AGGGGAACAG CGGTGAACCA GAGCCAGAAG GCCCCACAGA AGCAGCGGAC
    AGTCAGCCCT TGCGGTCCAG CTCTAGCACC ACAGCCAGTT CCAGCCCCAG TACAGTCATA
    CAGGGAGTCA ACCATGAGCA GAAGGAACCT GTTGAGGTGG AGCCCATAGC AAATGTGACT
    TACCCAAGCT CTGCCCCAGT ATTGGGTCCT CCTCCTTTTA CAAGACCCAC CCCTGATCCA
    ACCCTTGTTG ACCCTGCCAA TAAGAAGCGA GAATATGACA ACCCCTATTT TGAGCCCCAG
    TACGGTTTCC CTACAGAGGA AGATGCTGAG ACTGATGAGC AGGAGGAGAG TTACACACCA
    CGATTTAACC AGAATCTCAA TGGCAATAAG CCTTCTCGTC CACTGAGACC CAGCAGCCTG
    AAGCTTCCGG GTGAATCTGA CGGAGAGGGA GACTCCAGAA ATAGCTCTCC TAACTCAACC
    ATCTCCAACA ATAGCAGTGA TGGCTTTGGG GGACTCATGT CCTTTGCCAG TAACTTGTAT
    AAAAACCATG GCACCAGTTT CAGTCTGTCC AGCCTGGCTC TGCCTAATAA AGCGAGAGAG
    AAGAACACGC CGTTTCCCAG CCTCAAAGAC GATGCTGATG ACAGTCCTGA AAGTCCTGTA
    TTTGGGCTAA ATTCTCTAAT GGAGATTGTA ACAGAGATCG GCCCGGGGAG CGGAGAAGGT
    GCCCGAGCCC CCAGAGCACT GGTAGATCAG AAATCCTCTG TTATTAAGCA CAGCCCTACT
    GTGAAGAGAG AGTCTCCGTC TCCTCAAGGC AGAGCCAACA ATACCAGTGA GAACCAGCAG
    TTTCTAAAGG AGGTGGTCCA GAGCGTGCTG GAGGGGCAGG GTGTGGGCTG GCTTAACATG
    AAGAAGGTGC GTCGCCTGCT AGAGAATGAA CAGCTCAGAG TCTTTGTGCT CAGCAAACTC
    AACCGTGCGG TCCAGTCAGA GGAGGACGCC CAGCAGGAAG TTATAAGAGA TGTGGAGATA
    AATAGAAAGG TCTACAAAGG CATGCTGGAT ATTTTGAAGT GCACGGTGTC CAGTTTGGAG
    CATTCATACA CTAATGCTGG CTTAGGGGGC ATGGCCAGTG TCTTCAGTCT GCTAGAGATA
    GCACGCACCC ACTACCAAAC CAAAGATCCA GAGAAGCGGA AACGGAGCCC CACCGAAGGG
    CTTAGCAGTC CAGGCAGTAA GGAGAGCCCA TCGGGCCGAA TGGAGAGTGC CAGGGCGGCA
    GGGGTGCTTC TGGTACCCCG TATCCAATTG CCGCCACCCT CCTCTGGGAA ATCATCACGC
    CAGTTTGATA CCCGGAGTCT GAACGAGGAG AATTTTATTG CCTCCATTGA ATTGTGGAGC
    AAGCACCAGG ATAACAGAAA GCAAAAAGCT TTGGAAAAAG AACAGAGTGG TTTAAATAAA
    CGGAAATCAG GAGCGGATGG AGTAAAACAA CGTGTAGAAG GAGGAGACAC TGAGGAGAAG
    AAATCACAGA TCAGTGCAGA CAGTGGCCTC AGTGTGACAT CTGGCTCACA GAAGAGTGAT
    ACGGATTCCC TGGCGAGCTC TGAACCTCCT GCTCTGACCA GAAGCACCAG CCAGGACTCA
    GAGGCCAGCA CAGTGGTAAG TAACAGCTCA GGTGAGACGC TCGGTGCAGA CAGTGACCTG
    AGCAGCACTG CTGGAGATGG CCTGACAGGA AGACATGGTC AGCATCTCAA CCTGTCCCGT
    GGCACACTGT CTGACAGTGA GATTGAGACC AACCCTGCAA CCAGCTCAGT GTTTGGCAAG
    ACACACAAGC TGAAGCCAGG AGTGAAGGAG CCTGTTGGTG TTAATAAAGC AGCACCTGCT
    CCACCCGTTG AGGATGTTAG CATGAGGATC TATCTGTGTG AGGGGCTTCT GGGTAAAGAG
    CGCTCAACTC TGTGGGATCA GATGCAGTTC TGGGAGGACG CCTTCCTGGA TGCCGTAATG
    CTGGAACGGG AAGGAATGGG AATGGATCAG GGACCACAAG AAATGATTGA CAGGTATCTT
    TCTCTGGGAG ATCATGACCG CAAGAGACTG GAGGATGATG AGGACAGACT GCTGGCCACG
    CTTCTGCACA ATATGATTGC TTACATGCTT ATGATGAAGG TCGGTAAAAA CGACATCAGG
    AAGAAAGTAC GTCGTCTTAT GGGCAAATCC CACATTGGCC TCACTCACAG TCAGGAGATC
    AATGAAGTCC TGGATCGCCT CGCACATTTA AGCGGTCGGG AGCTTTCTAT CAGGCCGAGT
    GGCAGCAGGC ATATTAAAAA ACAGACATTT GTTGTGCATG CTGGGACAGA TACAACTGGT
    GACATATTCT TCATGGAGGT GTGTGATGAT TGCATCGTCT TGCGCAGTAA TATTGGCACA
    GTCTATGAGC GCTGGTGGTA TGAGAAGCTC ATAAATATGA CTTACTGTCC CAAAACTAAA
    GTGTTGTGTC TGTGGAGGAG AAACGGCCAG GAGACGCAGC TAAACAAGTT TTACACTAAG
    AAGTGTCGGG AATTGTACTA CTGTGTTAAA GACAGCATGG AGCGTGCTGC TGCTCGACAA
    CAAAGCATCA AACCAGGTCC AGAGCTTGGA GGCGAGTTTC CGGTTCAGGA CATGAAGACA
    GGGGAGGGTG GTCTCCTCCA AGTCACACTG GAAGGCATCA ACCTCAAATT TATGCACAGC
    CAGGAGCGGA AGGTTTTCAT AGAGCTGAAT CACATTAAAA AGTGCAATAC AGTGAAAGGA
    GTCTTTGTCC TGGAGGAGTT TGTTCCTGAA ACTAAAGAAG TGGTGATCCA TAAGTACAAG
    ACTCCTATGG CACATCAGAT CTGTTACTCG GTGCTCTGCC TCTTCTCCTA CATGGCGGCG
    GTCAAGGGGA AAGAGTCAGA GGGCAAACCC AAGATGCTGT CCCCTCGTCC ACTAGCGAGC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48922
    Clone ID Related Accession (Same CDS sequence) XM_009303273.2
    Accession Version XM_009303273.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5034bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product MAP kinase-activating death domain protein isoform X10
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303273.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGTG TCTGGTAAGG
    TTGACTCTGA TCACCCAAAA GCAGATCTTC GCCTCCGATT CTAAGGCTCT GGCTAGCATG
    AGTCTAAACA CACAGCCTAT TCTGAACCTG GAGAAGTTTC AGGATGGACA GGAGTTGTCT
    TTATTGCCCC CCGGTCGTGA CAAAGCTTCC CCGTCCTCCA CTGAGTTCAA CCCACTCATC
    TACGGCAATG ATGTGGACTC AGTTGATGTT GCCACAAGGG TGGCAATGGT GAGGTTCTTC
    AACTCTCCAA ACGTGCTGCA AGGGTTCCAG ATGCACACAC GCACTCTCCG TCTCTTCCCT
    CGTCCTGTGG TGGCCTTTCA GTCCACATCT TTCCTGGCTT CTAGGCCACG ACGAAATGGA
    TTCACAGAAA AGCTCTCCCA CACTCAGGCA GTGGAGTACT ATGGAGAATG GGCTCTCAAC
    CCCACCAACC TGGCTTTCCA AAGGATTCAT AACAATGTGT ATGACCCCTC TCTAATAGGC
    GATAAGCCTA AGTGGTATGC CCACCAGTTA CAGCCAGTAT TCTACCGCGT GTATGATGGA
    AACTCTCATT TAGCGGAGGC CTTGAGTGGA CCCCTGCAGG ATGAGACCAA TGATTCAGAC
    CCAACAGACG ACAGTGGCAG CGACAGTGAA GCATATGATG ACTCCAGCTC CTCCTACTCA
    TCTCTTGGAG ATTTTGTGAA TGAGATGATC AAAGGGGACA TACAGGGAGA CACACCAAAT
    GTTGATCCCC TGACCCATGC TGCACTGGGA GATGCAGATG AGGTTGAGAT CCATGACTTT
    CACGAGTATA AGGGGAACAG CGGTGAACCA GAGCCAGAAG GCCCCACAGA AGCAGCGGAC
    AGTCAGCCCT TGCGGTCCAG CTCTAGCACC ACAGCCAGTT CCAGCCCCAG TACAGTCATA
    CAGGGAGTCA ACCATGAGCA GAAGGAACCT GTTGAGGTGG AGCCCATAGC AAATGTGACT
    TACCCAAGCT CTGCCCCAGT ATTGGGTCCT CCTCCTTTTA CAAGACCCAC CCCTGATCCA
    ACCCTTGTTG ACCCTGCCAA TAAGAAGCGA GAATATGACA ACCCCTATTT TGAGCCCCAG
    TACGGTTTCC CTACAGAGGA AGATGCTGAG ACTGATGAGC AGGAGGAGAG TTACACACCA
    CGATTTAACC AGAATCTCAA TGGCAATAAG CCTTCTCGTC CACTGAGACC CAGCAGCCTG
    AAGCTTCCGG GTGAATCTGA CGGAGAGGGA GACTCCAGAA ATAGCTCTCC TAACTCAACC
    ATCTCCAACA ATAGCAGTGA TGGCTTTGGG GGACTCATGT CCTTTGCCAG TAACTTGTAT
    AAAAACCATG GCACCAGTTT CAGTCTGTCC AGCCTGGCTC TGCCTAATAA AGCGAGAGAG
    AAGAACACGC CGTTTCCCAG CCTCAAAGAC GATGCTGATG ACAGTCCTGA AAGTCCTGTA
    TTTGGGCTAA ATTCTCTAAT GGAGATTGTA ACAGAGATCG GCCCGGGGAG CGGAGAAGGT
    GCCCGAGCCC CCAGAGCACT GGTAGATCAG AAATCCTCTG TTATTAAGCA CAGCCCTACT
    GTGAAGAGAG AGTCTCCGTC TCCTCAAGGC AGAGCCAACA ATACCAGTGA GAACCAGCAG
    TTTCTAAAGG AGGTGGTCCA GAGCGTGCTG GAGGGGCAGG GTGTGGGCTG GCTTAACATG
    AAGAAGGTGC GTCGCCTGCT AGAGAATGAA CAGCTCAGAG TCTTTGTGCT CAGCAAACTC
    AACCGTGCGG TCCAGTCAGA GGAGGACGCC CAGCAGGAAG TTATAAGAGA TGTGGAGATA
    AATAGAAAGG TCTACAAAGG CATGCTGGAT ATTTTGAAGT GCACGGTGTC CAGTTTGGAG
    CATTCATACA CTAATGCTGG CTTAGGGGGC ATGGCCAGTG TCTTCAGTCT GCTAGAGATA
    GCACGCACCC ACTACCAAAC CAAAGATCCA GAGAAGCGGA AACGGAGCCC CACCGAAGGG
    CTTAGCAGTC CAGGCAGTAA GGAGAGCCCA TCGGGCCGAA TGGAGAGTGC CAGGGCGGCA
    GGGGTGCTTC TGGTACCCCG TATCCAATTG CCGCCACCCT CCTCTGGGAA ATCATCACGC
    CAGTTTGATA CCCGGAGTCT GAACGAGGAG AATTTTATTG CCTCCATTGA ATTGTGGAGC
    AAGCACCAGG ATAACAGAAA GCAAAAAGCT TTGGAAAAAG AACAGAGTGG TTTAAATAAA
    CGGAAATCAG GAGCGGATGG AGTAAAACAA CGTGTAGAAG GAGGAGACAC TGAGGAGAAG
    AAATCACAGA TCAGTGCAGA CAGTGGCCTC AGTGTGACAT CTGGCTCACA GAAGAGTGAT
    ACGGATTCCC TGGCGAGCTC TGAACCTCCT GCTCTGACCA GAAGCACCAG CCAGGACTCA
    GAGGCCAGCA CAGTGGTAAG TAACAGCTCA GGTGAGACGC TCGGTGCAGA CAGTGACCTG
    AGCAGCACTG CTGGAGATGG CCTGACAGGA AGACATGGTC AGCATCTCAA CCTGTCCCGT
    GGCACACTGT CTGACAGTGA GATTGAGACC AACCCTGCAA CCAGCTCAGT GTTTGGCAAG
    ACACACAAGC TGAAGCCAGG AGTGAAGGAG CCTGTTGGTG TTAATAAAGC AGCACCTGCT
    CCACCCGTTG AGGATGTTAG CATGAGGATC TATCTGTGTG AGGGGCTTCT GGGTAAAGAG
    CGCTCAACTC TGTGGGATCA GATGCAGTTC TGGGAGGACG CCTTCCTGGA TGCCGTAATG
    CTGGAACGGG AAGGAATGGG AATGGATCAG GGACCACAAG AAATGATTGA CAGGTATCTT
    TCTCTGGGAG ATCATGACCG CAAGAGACTG GAGGATGATG AGGACAGACT GCTGGCCACG
    CTTCTGCACA ATATGATTGC TTACATGCTT ATGATGAAGG TCGGTAAAAA CGACATCAGG
    AAGAAAGTAC GTCGTCTTAT GGGCAAATCC CACATTGGCC TCACTCACAG TCAGGAGATC
    AATGAAGTCC TGGATCGCCT CGCACATTTA AGCGGTCGGG AGCTTTCTAT CAGGCCGAGT
    GGCAGCAGGC ATATTAAAAA ACAGACATTT GTTGTGCATG CTGGGACAGA TACAACTGGT
    GACATATTCT TCATGGAGGT GTGTGATGAT TGCATCGTCT TGCGCAGTAA TATTGGCACA
    GTCTATGAGC GCTGGTGGTA TGAGAAGCTC ATAAATATGA CTTACTGTCC CAAAACTAAA
    GTGTTGTGTC TGTGGAGGAG AAACGGCCAG GAGACGCAGC TAAACAAGTT TTACACTAAG
    AAGTGTCGGG AATTGTACTA CTGTGTTAAA GACAGCATGG AGCGTGCTGC TGCTCGACAA
    CAAAGCATCA AACCAGGTCC AGAGCTTGGA GGCGAGTTTC CGGTTCAGGA CATGAAGACA
    GGGGAGGGTG GTCTCCTCCA AGTCACACTG GAAGGCATCA ACCTCAAATT TATGCACAGC
    CAGGTTTTCA TAGAGCTGAA TCACATTAAA AAGTGCAATA CAGTGAAAGG AGTCTTTGTC
    CTGGAGGAGT TTGTTCCTGA AACTAAAGAA GTGGTGATCC ATAAGTACAA GACTCCTATG
    GCACATCAGA TCTGTTACTC GGTGCTCTGC CTCTTCTCCT ACATGGCGGC GGTCAAGGGG
    AAAGAGTCAG AGGGCAAACC CAAGATGCTG TCCCCTCGTC CACTAGCGAG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301548.1
    CDS435..5468
    Translation

    Target ORF information:

    RefSeq Version XM_009303273.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303273.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGTG TCTGGTAAGG
    TTGACTCTGA TCACCCAAAA GCAGATCTTC GCCTCCGATT CTAAGGCTCT GGCTAGCATG
    AGTCTAAACA CACAGCCTAT TCTGAACCTG GAGAAGTTTC AGGATGGACA GGAGTTGTCT
    TTATTGCCCC CCGGTCGTGA CAAAGCTTCC CCGTCCTCCA CTGAGTTCAA CCCACTCATC
    TACGGCAATG ATGTGGACTC AGTTGATGTT GCCACAAGGG TGGCAATGGT GAGGTTCTTC
    AACTCTCCAA ACGTGCTGCA AGGGTTCCAG ATGCACACAC GCACTCTCCG TCTCTTCCCT
    CGTCCTGTGG TGGCCTTTCA GTCCACATCT TTCCTGGCTT CTAGGCCACG ACGAAATGGA
    TTCACAGAAA AGCTCTCCCA CACTCAGGCA GTGGAGTACT ATGGAGAATG GGCTCTCAAC
    CCCACCAACC TGGCTTTCCA AAGGATTCAT AACAATGTGT ATGACCCCTC TCTAATAGGC
    GATAAGCCTA AGTGGTATGC CCACCAGTTA CAGCCAGTAT TCTACCGCGT GTATGATGGA
    AACTCTCATT TAGCGGAGGC CTTGAGTGGA CCCCTGCAGG ATGAGACCAA TGATTCAGAC
    CCAACAGACG ACAGTGGCAG CGACAGTGAA GCATATGATG ACTCCAGCTC CTCCTACTCA
    TCTCTTGGAG ATTTTGTGAA TGAGATGATC AAAGGGGACA TACAGGGAGA CACACCAAAT
    GTTGATCCCC TGACCCATGC TGCACTGGGA GATGCAGATG AGGTTGAGAT CCATGACTTT
    CACGAGTATA AGGGGAACAG CGGTGAACCA GAGCCAGAAG GCCCCACAGA AGCAGCGGAC
    AGTCAGCCCT TGCGGTCCAG CTCTAGCACC ACAGCCAGTT CCAGCCCCAG TACAGTCATA
    CAGGGAGTCA ACCATGAGCA GAAGGAACCT GTTGAGGTGG AGCCCATAGC AAATGTGACT
    TACCCAAGCT CTGCCCCAGT ATTGGGTCCT CCTCCTTTTA CAAGACCCAC CCCTGATCCA
    ACCCTTGTTG ACCCTGCCAA TAAGAAGCGA GAATATGACA ACCCCTATTT TGAGCCCCAG
    TACGGTTTCC CTACAGAGGA AGATGCTGAG ACTGATGAGC AGGAGGAGAG TTACACACCA
    CGATTTAACC AGAATCTCAA TGGCAATAAG CCTTCTCGTC CACTGAGACC CAGCAGCCTG
    AAGCTTCCGG GTGAATCTGA CGGAGAGGGA GACTCCAGAA ATAGCTCTCC TAACTCAACC
    ATCTCCAACA ATAGCAGTGA TGGCTTTGGG GGACTCATGT CCTTTGCCAG TAACTTGTAT
    AAAAACCATG GCACCAGTTT CAGTCTGTCC AGCCTGGCTC TGCCTAATAA AGCGAGAGAG
    AAGAACACGC CGTTTCCCAG CCTCAAAGAC GATGCTGATG ACAGTCCTGA AAGTCCTGTA
    TTTGGGCTAA ATTCTCTAAT GGAGATTGTA ACAGAGATCG GCCCGGGGAG CGGAGAAGGT
    GCCCGAGCCC CCAGAGCACT GGTAGATCAG AAATCCTCTG TTATTAAGCA CAGCCCTACT
    GTGAAGAGAG AGTCTCCGTC TCCTCAAGGC AGAGCCAACA ATACCAGTGA GAACCAGCAG
    TTTCTAAAGG AGGTGGTCCA GAGCGTGCTG GAGGGGCAGG GTGTGGGCTG GCTTAACATG
    AAGAAGGTGC GTCGCCTGCT AGAGAATGAA CAGCTCAGAG TCTTTGTGCT CAGCAAACTC
    AACCGTGCGG TCCAGTCAGA GGAGGACGCC CAGCAGGAAG TTATAAGAGA TGTGGAGATA
    AATAGAAAGG TCTACAAAGG CATGCTGGAT ATTTTGAAGT GCACGGTGTC CAGTTTGGAG
    CATTCATACA CTAATGCTGG CTTAGGGGGC ATGGCCAGTG TCTTCAGTCT GCTAGAGATA
    GCACGCACCC ACTACCAAAC CAAAGATCCA GAGAAGCGGA AACGGAGCCC CACCGAAGGG
    CTTAGCAGTC CAGGCAGTAA GGAGAGCCCA TCGGGCCGAA TGGAGAGTGC CAGGGCGGCA
    GGGGTGCTTC TGGTACCCCG TATCCAATTG CCGCCACCCT CCTCTGGGAA ATCATCACGC
    CAGTTTGATA CCCGGAGTCT GAACGAGGAG AATTTTATTG CCTCCATTGA ATTGTGGAGC
    AAGCACCAGG ATAACAGAAA GCAAAAAGCT TTGGAAAAAG AACAGAGTGG TTTAAATAAA
    CGGAAATCAG GAGCGGATGG AGTAAAACAA CGTGTAGAAG GAGGAGACAC TGAGGAGAAG
    AAATCACAGA TCAGTGCAGA CAGTGGCCTC AGTGTGACAT CTGGCTCACA GAAGAGTGAT
    ACGGATTCCC TGGCGAGCTC TGAACCTCCT GCTCTGACCA GAAGCACCAG CCAGGACTCA
    GAGGCCAGCA CAGTGGTAAG TAACAGCTCA GGTGAGACGC TCGGTGCAGA CAGTGACCTG
    AGCAGCACTG CTGGAGATGG CCTGACAGGA AGACATGGTC AGCATCTCAA CCTGTCCCGT
    GGCACACTGT CTGACAGTGA GATTGAGACC AACCCTGCAA CCAGCTCAGT GTTTGGCAAG
    ACACACAAGC TGAAGCCAGG AGTGAAGGAG CCTGTTGGTG TTAATAAAGC AGCACCTGCT
    CCACCCGTTG AGGATGTTAG CATGAGGATC TATCTGTGTG AGGGGCTTCT GGGTAAAGAG
    CGCTCAACTC TGTGGGATCA GATGCAGTTC TGGGAGGACG CCTTCCTGGA TGCCGTAATG
    CTGGAACGGG AAGGAATGGG AATGGATCAG GGACCACAAG AAATGATTGA CAGGTATCTT
    TCTCTGGGAG ATCATGACCG CAAGAGACTG GAGGATGATG AGGACAGACT GCTGGCCACG
    CTTCTGCACA ATATGATTGC TTACATGCTT ATGATGAAGG TCGGTAAAAA CGACATCAGG
    AAGAAAGTAC GTCGTCTTAT GGGCAAATCC CACATTGGCC TCACTCACAG TCAGGAGATC
    AATGAAGTCC TGGATCGCCT CGCACATTTA AGCGGTCGGG AGCTTTCTAT CAGGCCGAGT
    GGCAGCAGGC ATATTAAAAA ACAGACATTT GTTGTGCATG CTGGGACAGA TACAACTGGT
    GACATATTCT TCATGGAGGT GTGTGATGAT TGCATCGTCT TGCGCAGTAA TATTGGCACA
    GTCTATGAGC GCTGGTGGTA TGAGAAGCTC ATAAATATGA CTTACTGTCC CAAAACTAAA
    GTGTTGTGTC TGTGGAGGAG AAACGGCCAG GAGACGCAGC TAAACAAGTT TTACACTAAG
    AAGTGTCGGG AATTGTACTA CTGTGTTAAA GACAGCATGG AGCGTGCTGC TGCTCGACAA
    CAAAGCATCA AACCAGGTCC AGAGCTTGGA GGCGAGTTTC CGGTTCAGGA CATGAAGACA
    GGGGAGGGTG GTCTCCTCCA AGTCACACTG GAAGGCATCA ACCTCAAATT TATGCACAGC
    CAGGTTTTCA TAGAGCTGAA TCACATTAAA AAGTGCAATA CAGTGAAAGG AGTCTTTGTC
    CTGGAGGAGT TTGTTCCTGA AACTAAAGAA GTGGTGATCC ATAAGTACAA GACTCCTATG
    GCACATCAGA TCTGTTACTC GGTGCTCTGC CTCTTCTCCT ACATGGCGGC GGTCAAGGGG
    AAAGAGTCAG AGGGCAAACC CAAGATGCTG TCCCCTCGTC CACTAGCGAG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48923
    Clone ID Related Accession (Same CDS sequence) XM_009303274.2
    Accession Version XM_009303274.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5031bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product MAP kinase-activating death domain protein isoform X11
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303274.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGGG AACATTCGGC GCCCCCTGCA
    GGAGATGGGA CTTTGCTGCC AGGCGAGCCG GGTAGCAGCG GGAAGTCTCC GCGATCTAAA
    CGCAGTGGCC GAATAGCACC ACAGAACCGC AACAGCATGT TAACATCTCT GTGTATTCTG
    AGCCACTACC CTTTCTTCTC AACATTCAGA GAGTGTCTGT ACATCCTCAA GCGCATGGTG
    GACTGCTGTA GTCAGCGGCT CAACCAGAGA CCCGGAGCTC CCAAATCCAC ACAACGGGAC
    ACGATGTGGC GTGTGTTCAC GGGAGCTCTT TCTGTGGAAG AAAAGGAGAA GGGCAGTCAG
    GTGCTCCAGG ACCTGAGGGA GATCGAGTCA TGGGTTTATC GGCTGCTGCG CTCACCGGTG
    CCTGTTGCAG GTCAGCGGCG TGTGGATGTG GAGGTCCTGC CCCATGAGCT GCAGCCTGCT
    CTGACATTTG CCCTTCCAGA CCCGTCCCGC TTCAGCATCG TAGACTTCCC GCTCCACCTG
    CCACTGGAGC TGCTGGGAGT CGATGCCTGT CTGCAGGTCT TGGCCTGCAT TCTGCTGGAG
    CACAAGGTGG TACTGCAGTC TCGAGATTAT AACGCTCTCT CAATGAGCGT CATGGCGTTT
    GTTTCGATGA TTTACCCTCT TGAGTACATG TTTCCTGTCA TTCCACTGTT GCCCACCTGC
    ATGGCCTCTG CAGAACAGCT CCTATTAGCA CCCACTCCAT ACGTCATTGG TGTGCCAGCC
    AGTTTCTTCT TGTACAAGTG TGATTTTAAG ATGCCTGATG ATGTTTGGTT AGTGGATCTG
    GACTGCAACA AGGTTATTGT TCCAAGCAAT GCAGAACTGC TCCCACCCTT ACCTGAACCT
    GAGGCCTCTG AGCTGAAGAA ACACTTGAAG CAGTGTCTGG TAAGGTTGAC TCTGATCACC
    CAAAAGCAGA TCTTCGCCTC CGATTCTAAG GCTCTGGCTA GCATGAGTCT AAACACACAG
    CCTATTCTGA ACCTGGAGAA GTTTCAGGAT GGACAGGAGT TGTCTTTATT GCCCCCCGGT
    CGTGACAAAG CTTCCCCGTC CTCCACTGAG TTCAACCCAC TCATCTACGG CAATGATGTG
    GACTCAGTTG ATGTTGCCAC AAGGGTGGCA ATGGTGAGGT TCTTCAACTC TCCAAACGTG
    CTGCAAGGGT TCCAGATGCA CACACGCACT CTCCGTCTCT TCCCTCGTCC TGTGGTGGCC
    TTTCAGTCCA CATCTTTCCT GGCTTCTAGG CCACGACGAA ATGGATTCAC AGAAAAGCTC
    TCCCACACTC AGGCAGTGGA GTACTATGGA GAATGGGCTC TCAACCCCAC CAACCTGGCT
    TTCCAAAGGA TTCATAACAA TGTGTATGAC CCCTCTCTAA TAGGCGATAA GCCTAAGTGG
    TATGCCCACC AGTTACAGCC AGTATTCTAC CGCGTGTATG ATGGAAACTC TCATTTAGCG
    GAGGCCTTGA GTGGACCCCT GCAGGATGAG ACCAATGATT CAGACCCAAC AGACGACAGT
    GGCAGCGACA GTGAAGCATA TGATGACTCC AGCTCCTCCT ACTCATCTCT TGGAGATTTT
    GTGAATGAGA TGATCAAAGG GGACATACAG GGAGACACAC CAAATGTTGA TCCCCTGACC
    CATGCTGCAC TGGGAGATGC AGATGAGGTT GAGATCCATG ACTTTCACGA GTATAAGGGG
    AACAGCGGTG AACCAGAGCC AGAAGGCCCC ACAGAAGCAG CGGACAGTCA GCCCTTGCGG
    TCCAGCTCTA GCACCACAGC CAGTTCCAGC CCCAGTACAG TCATACAGGG AGTCAACCAT
    GAGCAGAAGG AACCTGTTGA GGTGGAGCCC ATAGCAAATG TGACTTACCC AAGCTCTGCC
    CCAGTATTGG GTCCTCCTCC TTTTACAAGA CCCACCCCTG ATCCAACCCT TGTTGACCCT
    GCCAATAAGA AGCGAGAATA TGACAACCCC TATTTTGAGC CCCAGTACGG TTTCCCTACA
    GAGGAAGATG CTGAGACTGA TGAGCAGGAG GAGAGTTACA CACCACGATT TAACCAGAAT
    CTCAATGGCA ATAAGCCTTC TCGTCCACTG AGACCCAGCA GCCTGAAGCT TCCGGGTGAA
    TCTGACGGAG AGGGAGACTC CAGAAATAGC TCTCCTAACT CAACCATCTC CAACAATAGC
    AGTGATGGCT TTGGGGGACT CATGTCCTTT GCCAGTAACT TGTATAAAAA CCATGGCACC
    AGTTTCAGTC TGTCCAGCCT GGCTCTGCCT AATAAAGCGA GAGAGAAGAA CACGCCGTTT
    CCCAGCCTCA AAGACGATGC TGATGACAGT CCTGAAAGTC CTGTATTTGG GCTAAATTCT
    CTAATGGAGA TTGTAACAGA GATCGGCCCG GGGAGCGGAG AAGGTGCCCG AGCCCCCAGA
    GCACTGGTAG ATCAGAAATC CTCTGTTATT AAGCACAGCC CTACTGTGAA GAGAGAGTCT
    CCGTCTCCTC AAGGCAGAGC CAACAATACC AGTGAGAACC AGCAGTTTCT AAAGGAGGTG
    GTCCAGAGCG TGCTGGAGGG GCAGGGTGTG GGCTGGCTTA ACATGAAGAA GGTGCGTCGC
    CTGCTAGAGA ATGAACAGCT CAGAGTCTTT GTGCTCAGCA AACTCAACCG TGCGGTCCAG
    TCAGAGGAGG ACGCCCAGCA GGAAGTTATA AGAGATGTGG AGATAAATAG AAAGGTCTAC
    AAAGGCATGC TGGATATTTT GAAGTGCACG GTGTCCAGTT TGGAGCATTC ATACACTAAT
    GCTGGCTTAG GGGGCATGGC CAGTGTCTTC AGTCTGCTAG AGATAGCACG CACCCACTAC
    CAAACCAAAG ATCCAGAGAA GCGGAAACGG AGCCCCACCG AAGGGCTTAG CAGTCCAGGC
    AGTAAGGAGA GCCCATCGGG CCGAATGGAG AGTGCCAGGG CGGCAGGGGT GCTTCTGGTA
    CCCCGTATCC AATTGCCGCC ACCCTCCTCT GGGAAATCAT CACGCCAGTT TGATACCCGG
    AGTCTGAACG AGGAGAATTT TATTGCCTCC ATTGAATTGT GGAGCAAGCA CCAGGATAAC
    AGAAAGCAAA AAGCTTTGGA AAAAGAACAG AGTGGTTTAA ATAAACGGAA ATCAGGAGCG
    GATGGAGTAA AACAACGTGT AGAAGGAGGA GACACTGAGG AGAAGAAATC ACAGATCAGT
    GCAGACAGTG GCCTCAGTGT GACATCTGGC TCACAGAAGA GTGATACGGA TTCCCTGGCG
    AGCTCTGAAC CTCCTGCTCT GACCAGAAGC ACCAGCCAGG ACTCAGAGGC CAGCACAGTG
    GTAAGTAACA GCTCAGGTGA GACGCTCGGT GCAGACAGTG ACCTGAGCAG CACTGCTGGA
    GATGGCCTGA CAGGAAGACA TGGTCAGCAT CTCAACCTGT CCCGTGGCAC ACTGTCTGAC
    AGTGAGATTG AGACCAACCC TGCAACCAGC TCAGTGTTTG GCAAGACACA CAAGCTGAAG
    CCAGGAGTGA AGGAGCCTGT TGGTGTTAAT AAAGCAGCAC CTGCTCCACC CGTTGAGGAT
    GTTAGCATGA GGATCTATCT GTGTGAGGGG CTTCTGGGCC GGGATAAAAG CTCAGTTTGG
    GACCAGTTGG AGGATGCTGC AATGGAAACC TTTTCTCTGA GTAAAGAGCG CTCAACTCTG
    TGGGATCAGA TGCAGTTCTG GGAGGACGCC TTCCTGGATG CCGTAATGCT GGAACGGGAA
    GGAATGGGAA TGGATCAGGG ACCACAAGAA ATGATTGACA GGTATCTTTC TCTGGGAGAT
    CATGACCGCA AGAGACTGGA GGATGATGAG GACAGACTGC TGGCCACGCT TCTGCACAAT
    ATGATTGCTT ACATGCTTAT GATGAAGGTC GGTAAAAACG ACATCAGGAA GAAAGTACGT
    CGTCTTATGG GCAAATCCCA CATTGGCCTC ACTCACAGTC AGGAGATCAA TGAAGTCCTG
    GATCGCCTCG CACATTTAAG CGGTCGGGAG CTTTCTATCA GGCCGAGTGG CAGCAGGCAT
    ATTAAAAAAC AGACATTTGT TGTGCATGCT GGGACAGATA CAACTGGTGA CATATTCTTC
    ATGGAGGTGT GTGATGATTG CATCGTCTTG CGCAGTAATA TTGGCACAGT CTATGAGCGC
    TGGTGGTATG AGAAGCTCAT AAATATGACT TACTGTCCCA AAACTAAAGT GTTGTGTCTG
    TGGAGGAGAA ACGGCCAGGA GACGCAGCTA AACAAGTTTT ACACTAAGAA GTGTCGGGAA
    TTGTACTACT GTGTTAAAGA CAGCATGGAG CGTGCTGCTG CTCGACAACA AAGCATCAAA
    CCAGGTCCAG AGCTTGGAGG CGAGTTTCCG GTTCAGGACA TGAAGACAGG GGAGGGTGGT
    CTCCTCCAAG TCACACTGGA AGGCATCAAC CTCAAATTTA TGCACAGCCA GGAGCGGAAG
    GTTTTCATAG AGCTGAATCA CATTAAAAAG TGCAATACAG TGAAAGGAGT CTTTGTCCTG
    GAGGAGTTTG TTCCTGAAAC TAAAGAAGTG GTGATCCATA AGTACAAGAC TCCTATGGCA
    CATCAGATCT GTTACTCGGT GCTCTGCCTC TTCTCCTACA TGGCGGCGGT CAAGGGGAAA
    GAGTCAGAGG GCAAACCCAA GATGCTGTCC CCTCGTCCAC TAGCGAGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301549.1
    CDS435..5465
    Translation

    Target ORF information:

    RefSeq Version XM_009303274.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303274.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGGG AACATTCGGC GCCCCCTGCA
    GGAGATGGGA CTTTGCTGCC AGGCGAGCCG GGTAGCAGCG GGAAGTCTCC GCGATCTAAA
    CGCAGTGGCC GAATAGCACC ACAGAACCGC AACAGCATGT TAACATCTCT GTGTATTCTG
    AGCCACTACC CTTTCTTCTC AACATTCAGA GAGTGTCTGT ACATCCTCAA GCGCATGGTG
    GACTGCTGTA GTCAGCGGCT CAACCAGAGA CCCGGAGCTC CCAAATCCAC ACAACGGGAC
    ACGATGTGGC GTGTGTTCAC GGGAGCTCTT TCTGTGGAAG AAAAGGAGAA GGGCAGTCAG
    GTGCTCCAGG ACCTGAGGGA GATCGAGTCA TGGGTTTATC GGCTGCTGCG CTCACCGGTG
    CCTGTTGCAG GTCAGCGGCG TGTGGATGTG GAGGTCCTGC CCCATGAGCT GCAGCCTGCT
    CTGACATTTG CCCTTCCAGA CCCGTCCCGC TTCAGCATCG TAGACTTCCC GCTCCACCTG
    CCACTGGAGC TGCTGGGAGT CGATGCCTGT CTGCAGGTCT TGGCCTGCAT TCTGCTGGAG
    CACAAGGTGG TACTGCAGTC TCGAGATTAT AACGCTCTCT CAATGAGCGT CATGGCGTTT
    GTTTCGATGA TTTACCCTCT TGAGTACATG TTTCCTGTCA TTCCACTGTT GCCCACCTGC
    ATGGCCTCTG CAGAACAGCT CCTATTAGCA CCCACTCCAT ACGTCATTGG TGTGCCAGCC
    AGTTTCTTCT TGTACAAGTG TGATTTTAAG ATGCCTGATG ATGTTTGGTT AGTGGATCTG
    GACTGCAACA AGGTTATTGT TCCAAGCAAT GCAGAACTGC TCCCACCCTT ACCTGAACCT
    GAGGCCTCTG AGCTGAAGAA ACACTTGAAG CAGTGTCTGG TAAGGTTGAC TCTGATCACC
    CAAAAGCAGA TCTTCGCCTC CGATTCTAAG GCTCTGGCTA GCATGAGTCT AAACACACAG
    CCTATTCTGA ACCTGGAGAA GTTTCAGGAT GGACAGGAGT TGTCTTTATT GCCCCCCGGT
    CGTGACAAAG CTTCCCCGTC CTCCACTGAG TTCAACCCAC TCATCTACGG CAATGATGTG
    GACTCAGTTG ATGTTGCCAC AAGGGTGGCA ATGGTGAGGT TCTTCAACTC TCCAAACGTG
    CTGCAAGGGT TCCAGATGCA CACACGCACT CTCCGTCTCT TCCCTCGTCC TGTGGTGGCC
    TTTCAGTCCA CATCTTTCCT GGCTTCTAGG CCACGACGAA ATGGATTCAC AGAAAAGCTC
    TCCCACACTC AGGCAGTGGA GTACTATGGA GAATGGGCTC TCAACCCCAC CAACCTGGCT
    TTCCAAAGGA TTCATAACAA TGTGTATGAC CCCTCTCTAA TAGGCGATAA GCCTAAGTGG
    TATGCCCACC AGTTACAGCC AGTATTCTAC CGCGTGTATG ATGGAAACTC TCATTTAGCG
    GAGGCCTTGA GTGGACCCCT GCAGGATGAG ACCAATGATT CAGACCCAAC AGACGACAGT
    GGCAGCGACA GTGAAGCATA TGATGACTCC AGCTCCTCCT ACTCATCTCT TGGAGATTTT
    GTGAATGAGA TGATCAAAGG GGACATACAG GGAGACACAC CAAATGTTGA TCCCCTGACC
    CATGCTGCAC TGGGAGATGC AGATGAGGTT GAGATCCATG ACTTTCACGA GTATAAGGGG
    AACAGCGGTG AACCAGAGCC AGAAGGCCCC ACAGAAGCAG CGGACAGTCA GCCCTTGCGG
    TCCAGCTCTA GCACCACAGC CAGTTCCAGC CCCAGTACAG TCATACAGGG AGTCAACCAT
    GAGCAGAAGG AACCTGTTGA GGTGGAGCCC ATAGCAAATG TGACTTACCC AAGCTCTGCC
    CCAGTATTGG GTCCTCCTCC TTTTACAAGA CCCACCCCTG ATCCAACCCT TGTTGACCCT
    GCCAATAAGA AGCGAGAATA TGACAACCCC TATTTTGAGC CCCAGTACGG TTTCCCTACA
    GAGGAAGATG CTGAGACTGA TGAGCAGGAG GAGAGTTACA CACCACGATT TAACCAGAAT
    CTCAATGGCA ATAAGCCTTC TCGTCCACTG AGACCCAGCA GCCTGAAGCT TCCGGGTGAA
    TCTGACGGAG AGGGAGACTC CAGAAATAGC TCTCCTAACT CAACCATCTC CAACAATAGC
    AGTGATGGCT TTGGGGGACT CATGTCCTTT GCCAGTAACT TGTATAAAAA CCATGGCACC
    AGTTTCAGTC TGTCCAGCCT GGCTCTGCCT AATAAAGCGA GAGAGAAGAA CACGCCGTTT
    CCCAGCCTCA AAGACGATGC TGATGACAGT CCTGAAAGTC CTGTATTTGG GCTAAATTCT
    CTAATGGAGA TTGTAACAGA GATCGGCCCG GGGAGCGGAG AAGGTGCCCG AGCCCCCAGA
    GCACTGGTAG ATCAGAAATC CTCTGTTATT AAGCACAGCC CTACTGTGAA GAGAGAGTCT
    CCGTCTCCTC AAGGCAGAGC CAACAATACC AGTGAGAACC AGCAGTTTCT AAAGGAGGTG
    GTCCAGAGCG TGCTGGAGGG GCAGGGTGTG GGCTGGCTTA ACATGAAGAA GGTGCGTCGC
    CTGCTAGAGA ATGAACAGCT CAGAGTCTTT GTGCTCAGCA AACTCAACCG TGCGGTCCAG
    TCAGAGGAGG ACGCCCAGCA GGAAGTTATA AGAGATGTGG AGATAAATAG AAAGGTCTAC
    AAAGGCATGC TGGATATTTT GAAGTGCACG GTGTCCAGTT TGGAGCATTC ATACACTAAT
    GCTGGCTTAG GGGGCATGGC CAGTGTCTTC AGTCTGCTAG AGATAGCACG CACCCACTAC
    CAAACCAAAG ATCCAGAGAA GCGGAAACGG AGCCCCACCG AAGGGCTTAG CAGTCCAGGC
    AGTAAGGAGA GCCCATCGGG CCGAATGGAG AGTGCCAGGG CGGCAGGGGT GCTTCTGGTA
    CCCCGTATCC AATTGCCGCC ACCCTCCTCT GGGAAATCAT CACGCCAGTT TGATACCCGG
    AGTCTGAACG AGGAGAATTT TATTGCCTCC ATTGAATTGT GGAGCAAGCA CCAGGATAAC
    AGAAAGCAAA AAGCTTTGGA AAAAGAACAG AGTGGTTTAA ATAAACGGAA ATCAGGAGCG
    GATGGAGTAA AACAACGTGT AGAAGGAGGA GACACTGAGG AGAAGAAATC ACAGATCAGT
    GCAGACAGTG GCCTCAGTGT GACATCTGGC TCACAGAAGA GTGATACGGA TTCCCTGGCG
    AGCTCTGAAC CTCCTGCTCT GACCAGAAGC ACCAGCCAGG ACTCAGAGGC CAGCACAGTG
    GTAAGTAACA GCTCAGGTGA GACGCTCGGT GCAGACAGTG ACCTGAGCAG CACTGCTGGA
    GATGGCCTGA CAGGAAGACA TGGTCAGCAT CTCAACCTGT CCCGTGGCAC ACTGTCTGAC
    AGTGAGATTG AGACCAACCC TGCAACCAGC TCAGTGTTTG GCAAGACACA CAAGCTGAAG
    CCAGGAGTGA AGGAGCCTGT TGGTGTTAAT AAAGCAGCAC CTGCTCCACC CGTTGAGGAT
    GTTAGCATGA GGATCTATCT GTGTGAGGGG CTTCTGGGCC GGGATAAAAG CTCAGTTTGG
    GACCAGTTGG AGGATGCTGC AATGGAAACC TTTTCTCTGA GTAAAGAGCG CTCAACTCTG
    TGGGATCAGA TGCAGTTCTG GGAGGACGCC TTCCTGGATG CCGTAATGCT GGAACGGGAA
    GGAATGGGAA TGGATCAGGG ACCACAAGAA ATGATTGACA GGTATCTTTC TCTGGGAGAT
    CATGACCGCA AGAGACTGGA GGATGATGAG GACAGACTGC TGGCCACGCT TCTGCACAAT
    ATGATTGCTT ACATGCTTAT GATGAAGGTC GGTAAAAACG ACATCAGGAA GAAAGTACGT
    CGTCTTATGG GCAAATCCCA CATTGGCCTC ACTCACAGTC AGGAGATCAA TGAAGTCCTG
    GATCGCCTCG CACATTTAAG CGGTCGGGAG CTTTCTATCA GGCCGAGTGG CAGCAGGCAT
    ATTAAAAAAC AGACATTTGT TGTGCATGCT GGGACAGATA CAACTGGTGA CATATTCTTC
    ATGGAGGTGT GTGATGATTG CATCGTCTTG CGCAGTAATA TTGGCACAGT CTATGAGCGC
    TGGTGGTATG AGAAGCTCAT AAATATGACT TACTGTCCCA AAACTAAAGT GTTGTGTCTG
    TGGAGGAGAA ACGGCCAGGA GACGCAGCTA AACAAGTTTT ACACTAAGAA GTGTCGGGAA
    TTGTACTACT GTGTTAAAGA CAGCATGGAG CGTGCTGCTG CTCGACAACA AAGCATCAAA
    CCAGGTCCAG AGCTTGGAGG CGAGTTTCCG GTTCAGGACA TGAAGACAGG GGAGGGTGGT
    CTCCTCCAAG TCACACTGGA AGGCATCAAC CTCAAATTTA TGCACAGCCA GGAGCGGAAG
    GTTTTCATAG AGCTGAATCA CATTAAAAAG TGCAATACAG TGAAAGGAGT CTTTGTCCTG
    GAGGAGTTTG TTCCTGAAAC TAAAGAAGTG GTGATCCATA AGTACAAGAC TCCTATGGCA
    CATCAGATCT GTTACTCGGT GCTCTGCCTC TTCTCCTACA TGGCGGCGGT CAAGGGGAAA
    GAGTCAGAGG GCAAACCCAA GATGCTGTCC CCTCGTCCAC TAGCGAGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48924
    Clone ID Related Accession (Same CDS sequence) XM_009303275.2
    Accession Version XM_009303275.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5025bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product MAP kinase-activating death domain protein isoform X12
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303275.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGTG TCTGGTAAGG
    TTGACTCTGA TCACCCAAAA GCAGATCTTC GCCTCCGATT CTAAGGCTCT GGCTAGCATG
    AGTCTAAACA CACAGCCTAT TCTGAACCTG GAGAAGTTTC AGGATGGACA GGAGTTGTCT
    TTATTGCCCC CCGGTCGTGA CAAAGCTTCC CCGTCCTCCA CTGAGTTCAA CCCACTCATC
    TACGGCAATG ATGTGGACTC AGTTGATGTT GCCACAAGGG TGGCAATGGT GAGGTTCTTC
    AACTCTCCAA ACGTGCTGCA AGGGTTCCAG ATGCACACAC GCACTCTCCG TCTCTTCCCT
    CGTCCTGTGG TGGCCTTTCA GTCCACATCT TTCCTGGCTT CTAGGCCACG ACGAAATGGA
    TTCACAGAAA AGCTCTCCCA CACTCAGGCA GTGGAGTACT ATGGAGAATG GGCTCTCAAC
    CCCACCAACC TGGCTTTCCA AAGGATTCAT AACAATGTGT ATGACCCCTC TCTAATAGGC
    GATAAGCCTA AGTGGTATGC CCACCAGTTA CAGCCAGTAT TCTACCGCGT GTATGATGGA
    AACTCTCATT TAGCGGAGGC CTTGAGTGGA CCCCTGCAGG ATGAGACCAA TGATTCAGAC
    CCAACAGACG ACAGTGGCAG CGACAGTGAA GCATATGATG ACTCCAGCTC CTCCTACTCA
    TCTCTTGGAG ATTTTGTGAA TGAGATGATC AAAGGGGACA TACAGGGAGA CACACCAAAT
    GTTGATCCCC TGACCCATGC TGCACTGGGA GATGCAGATG AGGTTGAGAT CCATGACTTT
    CACGAGTATA AGGGGAACAG CGGTGAACCA GAGCCAGAAG GCCCCACAGA AGCAGCGGAC
    AGTCAGCCCT TGCGGTCCAG CTCTAGCACC ACAGCCAGTT CCAGCCCCAG TACAGTCATA
    CAGGGAGTCA ACCATGAGCA GAAGGAACCT GTTGAGGTGG AGCCCATAGC AAATGTGACT
    TACCCAAGCT CTGCCCCAGT ATTGGGTCCT CCTCCTTTTA CAAGACCCAC CCCTGATCCA
    ACCCTTGTTG ACCCTGCCAA TAAGAAGCGA GAATATGACA ACCCCTATTT TGAGCCCCAG
    TACGGTTTCC CTACAGAGGA AGATGCTGAG ACTGATGAGC AGGAGGAGAG TTACACACCA
    CGATTTAACC AGAATCTCAA TGGCAATAAG CCTTCTCGTC CACTGAGACC CAGCAGCCTG
    AAGCTTCCGG GTGAATCTGA CGGAGAGGGA GACTCCAGAA ATAGCTCTCC TAACTCAACC
    ATCTCCAACA ATAGCAGTGA TGGCTTTGGG GGACTCATGT CCTTTGCCAG TAACTTGTAT
    AAAAACCATG GCACCAGTTT CAGTCTGTCC AGCCTGGCTC TGCCTAATAA AGCGAGAGAG
    AAGAACACGC CGTTTCCCAG CCTCAAAGAC GATGCTGATG ACAGTCCTGA AAGTCCTGTA
    TTTGGGCTAA ATTCTCTAAT GGAGATTGTA ACAGAGATCG GCCCGGGGAG CGGAGAAGGT
    GCCCGAGCCC CCAGAGCACT GGTAGATCAG AAATCCTCTG TTATTAAGCA CAGCCCTACT
    GTGAAGAGAG AGTCTCCGTC TCCTCAAGGC AGAGCCAACA ATACCAGTGA GAACCAGCAG
    TTTCTAAAGG AGGTGGTCCA GAGCGTGCTG GAGGGGCAGG GTGTGGGCTG GCTTAACATG
    AAGAAGGTGC GTCGCCTGCT AGAGAATGAA CAGCTCAGAG TCTTTGTGCT CAGCAAACTC
    AACCGTGCGG TCCAGTCAGA GGAGGACGCC CAGCAGGAAG TTATAAGAGA TGTGGAGATA
    AATAGAAAGG TCTACAAAGG CATGCTGGAT ATTTTGAAGT GCACGGTGTC CAGTTTGGAG
    CATTCATACA CTAATGCTGG CTTAGGGGGC ATGGCCAGTG TCTTCAGTCT GCTAGAGATA
    GCACGCACCC ACTACCAAAC CAAAGATCCA GAGAAGCGGA AACGGAGCCC CACCGAAGGG
    CTTAGCAGTC CAGGCAGTAA GGAGAGCCCA TCGGGCCGAA TGGAGAGTGC CAGGGCGGCA
    GGGGTGCTTC TGGTACCCCG TATCCAATTG CCGCCACCCT CCTCTGGGAA ATCATCACGC
    CAGTTTGATA CCCGGAGTCT GAACGAGGAG AATTTTATTG CCTCCATTGG AGCGGATGGA
    GTAAAACAAC GTGTAGAAGG AGGAGACACT GAGGAGAAGA AATCACAGAT CAGTGCAGAC
    AGTGGCCTCA GTGTGACATC TGGCTCACAG AAGAGTGATA CGGATTCCCT GGCGAGCTCT
    GAACCTCCTG CTCTGACCAG AAGCACCAGC CAGGACTCAG AGGCCAGCAC AGTGGTAAGT
    AACAGCTCAG GTGAGACGCT CGGTGCAGAC AGTGACCTGA GCAGCACTGC TGGAGATGGC
    CTGACAGGAA GACATGGTCA GCATCTCAAC CTGTCCCGTG GCACACTGTC TGACAGTGAG
    ATTGAGACCA ACCCTGCAAC CAGCTCAGTG TTTGGCAAGA CACACAAGCT GAAGCCAGGA
    GTGAAGGAGC CTGTTGGTGT TAATAAAGCA GCACCTGCTC CACCCGTTGA GGATGTTAGC
    ATGAGGATCT ATCTGTGTGA GGGGCTTCTG GGCCGGGATA AAAGCTCAGT TTGGGACCAG
    TTGGAGGATG CTGCAATGGA AACCTTTTCT CTGAGTAAAG AGCGCTCAAC TCTGTGGGAT
    CAGATGCAGT TCTGGGAGGA CGCCTTCCTG GATGCCGTAA TGCTGGAACG GGAAGGAATG
    GGAATGGATC AGGGACCACA AGAAATGATT GACAGGTATC TTTCTCTGGG AGATCATGAC
    CGCAAGAGAC TGGAGGATGA TGAGGACAGA CTGCTGGCCA CGCTTCTGCA CAATATGATT
    GCTTACATGC TTATGATGAA GGTCGGTAAA AACGACATCA GGAAGAAAGT ACGTCGTCTT
    ATGGGCAAAT CCCACATTGG CCTCACTCAC AGTCAGGAGA TCAATGAAGT CCTGGATCGC
    CTCGCACATT TAAGCGGTCG GGAGCTTTCT ATCAGGCCGA GTGGCAGCAG GCATATTAAA
    AAACAGACAT TTGTTGTGCA TGCTGGGACA GATACAACTG GTGACATATT CTTCATGGAG
    GTGTGTGATG ATTGCATCGT CTTGCGCAGT AATATTGGCA CAGTCTATGA GCGCTGGTGG
    TATGAGAAGC TCATAAATAT GACTTACTGT CCCAAAACTA AAGTGTTGTG TCTGTGGAGG
    AGAAACGGCC AGGAGACGCA GCTAAACAAG TTTTACACTA AGAAGTGTCG GGAATTGTAC
    TACTGTGTTA AAGACAGCAT GGAGCGTGCT GCTGCTCGAC AACAAAGCAT CAAACCAGGT
    CCAGAGCTTG GAGGCGAGTT TCCGGTTCAG GACATGAAGA CAGGGGAGGG TGGTCTCCTC
    CAAGTCACAC TGGAAGGCAT CAACCTCAAA TTTATGCACA GCCAGGAGCG GAAGGTTTTC
    ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTGAAAG GAGTCTTTGT CCTGGAGGAG
    TTTGTTCCTG AAACTAAAGA AGTGGTGATC CATAAGTACA AGACTCCTAT GGCACATCAG
    ATCTGTTACT CGGTGCTCTG CCTCTTCTCC TACATGGCGG CGGTCAAGGG GAAAGAGTCA
    GAGGGCAAAC CCAAGATGCT GTCCCCTCGT CCACTAGCGA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301550.1
    CDS435..5459
    Translation

    Target ORF information:

    RefSeq Version XM_009303275.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303275.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGTG TCTGGTAAGG
    TTGACTCTGA TCACCCAAAA GCAGATCTTC GCCTCCGATT CTAAGGCTCT GGCTAGCATG
    AGTCTAAACA CACAGCCTAT TCTGAACCTG GAGAAGTTTC AGGATGGACA GGAGTTGTCT
    TTATTGCCCC CCGGTCGTGA CAAAGCTTCC CCGTCCTCCA CTGAGTTCAA CCCACTCATC
    TACGGCAATG ATGTGGACTC AGTTGATGTT GCCACAAGGG TGGCAATGGT GAGGTTCTTC
    AACTCTCCAA ACGTGCTGCA AGGGTTCCAG ATGCACACAC GCACTCTCCG TCTCTTCCCT
    CGTCCTGTGG TGGCCTTTCA GTCCACATCT TTCCTGGCTT CTAGGCCACG ACGAAATGGA
    TTCACAGAAA AGCTCTCCCA CACTCAGGCA GTGGAGTACT ATGGAGAATG GGCTCTCAAC
    CCCACCAACC TGGCTTTCCA AAGGATTCAT AACAATGTGT ATGACCCCTC TCTAATAGGC
    GATAAGCCTA AGTGGTATGC CCACCAGTTA CAGCCAGTAT TCTACCGCGT GTATGATGGA
    AACTCTCATT TAGCGGAGGC CTTGAGTGGA CCCCTGCAGG ATGAGACCAA TGATTCAGAC
    CCAACAGACG ACAGTGGCAG CGACAGTGAA GCATATGATG ACTCCAGCTC CTCCTACTCA
    TCTCTTGGAG ATTTTGTGAA TGAGATGATC AAAGGGGACA TACAGGGAGA CACACCAAAT
    GTTGATCCCC TGACCCATGC TGCACTGGGA GATGCAGATG AGGTTGAGAT CCATGACTTT
    CACGAGTATA AGGGGAACAG CGGTGAACCA GAGCCAGAAG GCCCCACAGA AGCAGCGGAC
    AGTCAGCCCT TGCGGTCCAG CTCTAGCACC ACAGCCAGTT CCAGCCCCAG TACAGTCATA
    CAGGGAGTCA ACCATGAGCA GAAGGAACCT GTTGAGGTGG AGCCCATAGC AAATGTGACT
    TACCCAAGCT CTGCCCCAGT ATTGGGTCCT CCTCCTTTTA CAAGACCCAC CCCTGATCCA
    ACCCTTGTTG ACCCTGCCAA TAAGAAGCGA GAATATGACA ACCCCTATTT TGAGCCCCAG
    TACGGTTTCC CTACAGAGGA AGATGCTGAG ACTGATGAGC AGGAGGAGAG TTACACACCA
    CGATTTAACC AGAATCTCAA TGGCAATAAG CCTTCTCGTC CACTGAGACC CAGCAGCCTG
    AAGCTTCCGG GTGAATCTGA CGGAGAGGGA GACTCCAGAA ATAGCTCTCC TAACTCAACC
    ATCTCCAACA ATAGCAGTGA TGGCTTTGGG GGACTCATGT CCTTTGCCAG TAACTTGTAT
    AAAAACCATG GCACCAGTTT CAGTCTGTCC AGCCTGGCTC TGCCTAATAA AGCGAGAGAG
    AAGAACACGC CGTTTCCCAG CCTCAAAGAC GATGCTGATG ACAGTCCTGA AAGTCCTGTA
    TTTGGGCTAA ATTCTCTAAT GGAGATTGTA ACAGAGATCG GCCCGGGGAG CGGAGAAGGT
    GCCCGAGCCC CCAGAGCACT GGTAGATCAG AAATCCTCTG TTATTAAGCA CAGCCCTACT
    GTGAAGAGAG AGTCTCCGTC TCCTCAAGGC AGAGCCAACA ATACCAGTGA GAACCAGCAG
    TTTCTAAAGG AGGTGGTCCA GAGCGTGCTG GAGGGGCAGG GTGTGGGCTG GCTTAACATG
    AAGAAGGTGC GTCGCCTGCT AGAGAATGAA CAGCTCAGAG TCTTTGTGCT CAGCAAACTC
    AACCGTGCGG TCCAGTCAGA GGAGGACGCC CAGCAGGAAG TTATAAGAGA TGTGGAGATA
    AATAGAAAGG TCTACAAAGG CATGCTGGAT ATTTTGAAGT GCACGGTGTC CAGTTTGGAG
    CATTCATACA CTAATGCTGG CTTAGGGGGC ATGGCCAGTG TCTTCAGTCT GCTAGAGATA
    GCACGCACCC ACTACCAAAC CAAAGATCCA GAGAAGCGGA AACGGAGCCC CACCGAAGGG
    CTTAGCAGTC CAGGCAGTAA GGAGAGCCCA TCGGGCCGAA TGGAGAGTGC CAGGGCGGCA
    GGGGTGCTTC TGGTACCCCG TATCCAATTG CCGCCACCCT CCTCTGGGAA ATCATCACGC
    CAGTTTGATA CCCGGAGTCT GAACGAGGAG AATTTTATTG CCTCCATTGG AGCGGATGGA
    GTAAAACAAC GTGTAGAAGG AGGAGACACT GAGGAGAAGA AATCACAGAT CAGTGCAGAC
    AGTGGCCTCA GTGTGACATC TGGCTCACAG AAGAGTGATA CGGATTCCCT GGCGAGCTCT
    GAACCTCCTG CTCTGACCAG AAGCACCAGC CAGGACTCAG AGGCCAGCAC AGTGGTAAGT
    AACAGCTCAG GTGAGACGCT CGGTGCAGAC AGTGACCTGA GCAGCACTGC TGGAGATGGC
    CTGACAGGAA GACATGGTCA GCATCTCAAC CTGTCCCGTG GCACACTGTC TGACAGTGAG
    ATTGAGACCA ACCCTGCAAC CAGCTCAGTG TTTGGCAAGA CACACAAGCT GAAGCCAGGA
    GTGAAGGAGC CTGTTGGTGT TAATAAAGCA GCACCTGCTC CACCCGTTGA GGATGTTAGC
    ATGAGGATCT ATCTGTGTGA GGGGCTTCTG GGCCGGGATA AAAGCTCAGT TTGGGACCAG
    TTGGAGGATG CTGCAATGGA AACCTTTTCT CTGAGTAAAG AGCGCTCAAC TCTGTGGGAT
    CAGATGCAGT TCTGGGAGGA CGCCTTCCTG GATGCCGTAA TGCTGGAACG GGAAGGAATG
    GGAATGGATC AGGGACCACA AGAAATGATT GACAGGTATC TTTCTCTGGG AGATCATGAC
    CGCAAGAGAC TGGAGGATGA TGAGGACAGA CTGCTGGCCA CGCTTCTGCA CAATATGATT
    GCTTACATGC TTATGATGAA GGTCGGTAAA AACGACATCA GGAAGAAAGT ACGTCGTCTT
    ATGGGCAAAT CCCACATTGG CCTCACTCAC AGTCAGGAGA TCAATGAAGT CCTGGATCGC
    CTCGCACATT TAAGCGGTCG GGAGCTTTCT ATCAGGCCGA GTGGCAGCAG GCATATTAAA
    AAACAGACAT TTGTTGTGCA TGCTGGGACA GATACAACTG GTGACATATT CTTCATGGAG
    GTGTGTGATG ATTGCATCGT CTTGCGCAGT AATATTGGCA CAGTCTATGA GCGCTGGTGG
    TATGAGAAGC TCATAAATAT GACTTACTGT CCCAAAACTA AAGTGTTGTG TCTGTGGAGG
    AGAAACGGCC AGGAGACGCA GCTAAACAAG TTTTACACTA AGAAGTGTCG GGAATTGTAC
    TACTGTGTTA AAGACAGCAT GGAGCGTGCT GCTGCTCGAC AACAAAGCAT CAAACCAGGT
    CCAGAGCTTG GAGGCGAGTT TCCGGTTCAG GACATGAAGA CAGGGGAGGG TGGTCTCCTC
    CAAGTCACAC TGGAAGGCAT CAACCTCAAA TTTATGCACA GCCAGGAGCG GAAGGTTTTC
    ATAGAGCTGA ATCACATTAA AAAGTGCAAT ACAGTGAAAG GAGTCTTTGT CCTGGAGGAG
    TTTGTTCCTG AAACTAAAGA AGTGGTGATC CATAAGTACA AGACTCCTAT GGCACATCAG
    ATCTGTTACT CGGTGCTCTG CCTCTTCTCC TACATGGCGG CGGTCAAGGG GAAAGAGTCA
    GAGGGCAAAC CCAAGATGCT GTCCCCTCGT CCACTAGCGA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48925
    Clone ID Related Accession (Same CDS sequence) XM_009303276.2 , XM_009303276.3
    Accession Version XM_009303276.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5019bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product MAP kinase-activating death domain protein isoform X13
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303276.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GTATTTGGGC TAAATTCTCT AATGGAGATT
    GTAACAGAGA TCGGCCCGGG GAGCGGAGAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGAATTGTGG AGCAAGCACC AGGATAACAG AAAGCAAAAA
    GCTTTGGAAA AAGAACAGAG TGGTTTAAAT AAACGGAAAT CAGGAGCGGA TGGAGTAAAA
    CAACGTGTAG AAGGAGGAGA CACTGAGGAG AAGAAATCAC AGATCAGTGC AGACAGTGGC
    CTCAGTGTGA CATCTGGCTC ACAGAAGAGT GATACGGATT CCCTGGCGAG CTCTGAACCT
    CCTGCTCTGA CCAGAAGCAC CAGCCAGGAC TCAGAGGCCA GCACAGTGGT AAGTAACAGC
    TCAGGTGAGA CGCTCGGTGC AGACAGTGAC CTGAGCAGCA CTGCTGGAGA TGGCCTGACA
    GGAAGACATG GTCAGCATCT CAACCTGTCC CGTGGCACAC TGTCTGACAG TGAGATTGAG
    ACCAACCCTG CAACCAGCTC AGTGTTTGGC AAGACACACA AGCTGAAGCC AGGAGTGAAG
    GAGCCTGTTG GTGTTAATAA AGCAGCACCT GCTCCACCCG TTGAGGATGT TAGCATGAGG
    ATCTATCTGT GTGAGGGGCT TCTGGGCCGG GATAAAAGCT CAGTTTGGGA CCAGTTGGAG
    GATGCTGCAA TGGAAACCTT TTCTCTGAGT AAAGAGCGCT CAACTCTGTG GGATCAGATG
    CAGTTCTGGG AGGACGCCTT CCTGGATGCC GTAATGCTGG AACGGGAAGG AATGGGAATG
    GATCAGGGAC CACAAGAAAT GATTGACAGG TATCTTTCTC TGGGAGATCA TGACCGCAAG
    AGACTGGAGG ATGATGAGGA CAGACTGCTG GCCACGCTTC TGCACAATAT GATTGCTTAC
    ATGCTTATGA TGAAGGTCGG TAAAAACGAC ATCAGGAAGA AAGTACGTCG TCTTATGGGC
    AAATCCCACA TTGGCCTCAC TCACAGTCAG GAGATCAATG AAGTCCTGGA TCGCCTCGCA
    CATTTAAGCG GTCGGGAGCT TTCTATCAGG CCGAGTGGCA GCAGGCATAT TAAAAAACAG
    ACATTTGTTG TGCATGCTGG GACAGATACA ACTGGTGACA TATTCTTCAT GGAGGTGTGT
    GATGATTGCA TCGTCTTGCG CAGTAATATT GGCACAGTCT ATGAGCGCTG GTGGTATGAG
    AAGCTCATAA ATATGACTTA CTGTCCCAAA ACTAAAGTGT TGTGTCTGTG GAGGAGAAAC
    GGCCAGGAGA CGCAGCTAAA CAAGTTTTAC ACTAAGAAGT GTCGGGAATT GTACTACTGT
    GTTAAAGACA GCATGGAGCG TGCTGCTGCT CGACAACAAA GCATCAAACC AGGTCCAGAG
    CTTGGAGGCG AGTTTCCGGT TCAGGACATG AAGACAGGGG AGGGTGGTCT CCTCCAAGTC
    ACACTGGAAG GCATCAACCT CAAATTTATG CACAGCCAGG AGCGGAAGGT TTTCATAGAG
    CTGAATCACA TTAAAAAGTG CAATACAGTG AAAGGAGTCT TTGTCCTGGA GGAGTTTGTT
    CCTGAAACTA AAGAAGTGGT GATCCATAAG TACAAGACTC CTATGGCACA TCAGATCTGT
    TACTCGGTGC TCTGCCTCTT CTCCTACATG GCGGCGGTCA AGGGGAAAGA GTCAGAGGGC
    AAACCCAAGA TGCTGTCCCC TCGTCCACTA GCGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301551.1
    CDS435..5453
    Translation

    Target ORF information:

    RefSeq Version XM_009303276.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303276.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GTATTTGGGC TAAATTCTCT AATGGAGATT
    GTAACAGAGA TCGGCCCGGG GAGCGGAGAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGAATTGTGG AGCAAGCACC AGGATAACAG AAAGCAAAAA
    GCTTTGGAAA AAGAACAGAG TGGTTTAAAT AAACGGAAAT CAGGAGCGGA TGGAGTAAAA
    CAACGTGTAG AAGGAGGAGA CACTGAGGAG AAGAAATCAC AGATCAGTGC AGACAGTGGC
    CTCAGTGTGA CATCTGGCTC ACAGAAGAGT GATACGGATT CCCTGGCGAG CTCTGAACCT
    CCTGCTCTGA CCAGAAGCAC CAGCCAGGAC TCAGAGGCCA GCACAGTGGT AAGTAACAGC
    TCAGGTGAGA CGCTCGGTGC AGACAGTGAC CTGAGCAGCA CTGCTGGAGA TGGCCTGACA
    GGAAGACATG GTCAGCATCT CAACCTGTCC CGTGGCACAC TGTCTGACAG TGAGATTGAG
    ACCAACCCTG CAACCAGCTC AGTGTTTGGC AAGACACACA AGCTGAAGCC AGGAGTGAAG
    GAGCCTGTTG GTGTTAATAA AGCAGCACCT GCTCCACCCG TTGAGGATGT TAGCATGAGG
    ATCTATCTGT GTGAGGGGCT TCTGGGCCGG GATAAAAGCT CAGTTTGGGA CCAGTTGGAG
    GATGCTGCAA TGGAAACCTT TTCTCTGAGT AAAGAGCGCT CAACTCTGTG GGATCAGATG
    CAGTTCTGGG AGGACGCCTT CCTGGATGCC GTAATGCTGG AACGGGAAGG AATGGGAATG
    GATCAGGGAC CACAAGAAAT GATTGACAGG TATCTTTCTC TGGGAGATCA TGACCGCAAG
    AGACTGGAGG ATGATGAGGA CAGACTGCTG GCCACGCTTC TGCACAATAT GATTGCTTAC
    ATGCTTATGA TGAAGGTCGG TAAAAACGAC ATCAGGAAGA AAGTACGTCG TCTTATGGGC
    AAATCCCACA TTGGCCTCAC TCACAGTCAG GAGATCAATG AAGTCCTGGA TCGCCTCGCA
    CATTTAAGCG GTCGGGAGCT TTCTATCAGG CCGAGTGGCA GCAGGCATAT TAAAAAACAG
    ACATTTGTTG TGCATGCTGG GACAGATACA ACTGGTGACA TATTCTTCAT GGAGGTGTGT
    GATGATTGCA TCGTCTTGCG CAGTAATATT GGCACAGTCT ATGAGCGCTG GTGGTATGAG
    AAGCTCATAA ATATGACTTA CTGTCCCAAA ACTAAAGTGT TGTGTCTGTG GAGGAGAAAC
    GGCCAGGAGA CGCAGCTAAA CAAGTTTTAC ACTAAGAAGT GTCGGGAATT GTACTACTGT
    GTTAAAGACA GCATGGAGCG TGCTGCTGCT CGACAACAAA GCATCAAACC AGGTCCAGAG
    CTTGGAGGCG AGTTTCCGGT TCAGGACATG AAGACAGGGG AGGGTGGTCT CCTCCAAGTC
    ACACTGGAAG GCATCAACCT CAAATTTATG CACAGCCAGG AGCGGAAGGT TTTCATAGAG
    CTGAATCACA TTAAAAAGTG CAATACAGTG AAAGGAGTCT TTGTCCTGGA GGAGTTTGTT
    CCTGAAACTA AAGAAGTGGT GATCCATAAG TACAAGACTC CTATGGCACA TCAGATCTGT
    TACTCGGTGC TCTGCCTCTT CTCCTACATG GCGGCGGTCA AGGGGAAAGA GTCAGAGGGC
    AAACCCAAGA TGCTGTCCCC TCGTCCACTA GCGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48925
    Clone ID Related Accession (Same CDS sequence) XM_009303276.2 , XM_009303276.3
    Accession Version XM_009303276.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5019bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303276.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GTATTTGGGC TAAATTCTCT AATGGAGATT
    GTAACAGAGA TCGGCCCGGG GAGCGGAGAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGAATTGTGG AGCAAGCACC AGGATAACAG AAAGCAAAAA
    GCTTTGGAAA AAGAACAGAG TGGTTTAAAT AAACGGAAAT CAGGAGCGGA TGGAGTAAAA
    CAACGTGTAG AAGGAGGAGA CACTGAGGAG AAGAAATCAC AGATCAGTGC AGACAGTGGC
    CTCAGTGTGA CATCTGGCTC ACAGAAGAGT GATACGGATT CCCTGGCGAG CTCTGAACCT
    CCTGCTCTGA CCAGAAGCAC CAGCCAGGAC TCAGAGGCCA GCACAGTGGT AAGTAACAGC
    TCAGGTGAGA CGCTCGGTGC AGACAGTGAC CTGAGCAGCA CTGCTGGAGA TGGCCTGACA
    GGAAGACATG GTCAGCATCT CAACCTGTCC CGTGGCACAC TGTCTGACAG TGAGATTGAG
    ACCAACCCTG CAACCAGCTC AGTGTTTGGC AAGACACACA AGCTGAAGCC AGGAGTGAAG
    GAGCCTGTTG GTGTTAATAA AGCAGCACCT GCTCCACCCG TTGAGGATGT TAGCATGAGG
    ATCTATCTGT GTGAGGGGCT TCTGGGCCGG GATAAAAGCT CAGTTTGGGA CCAGTTGGAG
    GATGCTGCAA TGGAAACCTT TTCTCTGAGT AAAGAGCGCT CAACTCTGTG GGATCAGATG
    CAGTTCTGGG AGGACGCCTT CCTGGATGCC GTAATGCTGG AACGGGAAGG AATGGGAATG
    GATCAGGGAC CACAAGAAAT GATTGACAGG TATCTTTCTC TGGGAGATCA TGACCGCAAG
    AGACTGGAGG ATGATGAGGA CAGACTGCTG GCCACGCTTC TGCACAATAT GATTGCTTAC
    ATGCTTATGA TGAAGGTCGG TAAAAACGAC ATCAGGAAGA AAGTACGTCG TCTTATGGGC
    AAATCCCACA TTGGCCTCAC TCACAGTCAG GAGATCAATG AAGTCCTGGA TCGCCTCGCA
    CATTTAAGCG GTCGGGAGCT TTCTATCAGG CCGAGTGGCA GCAGGCATAT TAAAAAACAG
    ACATTTGTTG TGCATGCTGG GACAGATACA ACTGGTGACA TATTCTTCAT GGAGGTGTGT
    GATGATTGCA TCGTCTTGCG CAGTAATATT GGCACAGTCT ATGAGCGCTG GTGGTATGAG
    AAGCTCATAA ATATGACTTA CTGTCCCAAA ACTAAAGTGT TGTGTCTGTG GAGGAGAAAC
    GGCCAGGAGA CGCAGCTAAA CAAGTTTTAC ACTAAGAAGT GTCGGGAATT GTACTACTGT
    GTTAAAGACA GCATGGAGCG TGCTGCTGCT CGACAACAAA GCATCAAACC AGGTCCAGAG
    CTTGGAGGCG AGTTTCCGGT TCAGGACATG AAGACAGGGG AGGGTGGTCT CCTCCAAGTC
    ACACTGGAAG GCATCAACCT CAAATTTATG CACAGCCAGG AGCGGAAGGT TTTCATAGAG
    CTGAATCACA TTAAAAAGTG CAATACAGTG AAAGGAGTCT TTGTCCTGGA GGAGTTTGTT
    CCTGAAACTA AAGAAGTGGT GATCCATAAG TACAAGACTC CTATGGCACA TCAGATCTGT
    TACTCGGTGC TCTGCCTCTT CTCCTACATG GCGGCGGTCA AGGGGAAAGA GTCAGAGGGC
    AAACCCAAGA TGCTGTCCCC TCGTCCACTA GCGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301551.1
    CDS432..5450
    Translation

    Target ORF information:

    RefSeq Version XM_009303276.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303276.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GTATTTGGGC TAAATTCTCT AATGGAGATT
    GTAACAGAGA TCGGCCCGGG GAGCGGAGAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGAATTGTGG AGCAAGCACC AGGATAACAG AAAGCAAAAA
    GCTTTGGAAA AAGAACAGAG TGGTTTAAAT AAACGGAAAT CAGGAGCGGA TGGAGTAAAA
    CAACGTGTAG AAGGAGGAGA CACTGAGGAG AAGAAATCAC AGATCAGTGC AGACAGTGGC
    CTCAGTGTGA CATCTGGCTC ACAGAAGAGT GATACGGATT CCCTGGCGAG CTCTGAACCT
    CCTGCTCTGA CCAGAAGCAC CAGCCAGGAC TCAGAGGCCA GCACAGTGGT AAGTAACAGC
    TCAGGTGAGA CGCTCGGTGC AGACAGTGAC CTGAGCAGCA CTGCTGGAGA TGGCCTGACA
    GGAAGACATG GTCAGCATCT CAACCTGTCC CGTGGCACAC TGTCTGACAG TGAGATTGAG
    ACCAACCCTG CAACCAGCTC AGTGTTTGGC AAGACACACA AGCTGAAGCC AGGAGTGAAG
    GAGCCTGTTG GTGTTAATAA AGCAGCACCT GCTCCACCCG TTGAGGATGT TAGCATGAGG
    ATCTATCTGT GTGAGGGGCT TCTGGGCCGG GATAAAAGCT CAGTTTGGGA CCAGTTGGAG
    GATGCTGCAA TGGAAACCTT TTCTCTGAGT AAAGAGCGCT CAACTCTGTG GGATCAGATG
    CAGTTCTGGG AGGACGCCTT CCTGGATGCC GTAATGCTGG AACGGGAAGG AATGGGAATG
    GATCAGGGAC CACAAGAAAT GATTGACAGG TATCTTTCTC TGGGAGATCA TGACCGCAAG
    AGACTGGAGG ATGATGAGGA CAGACTGCTG GCCACGCTTC TGCACAATAT GATTGCTTAC
    ATGCTTATGA TGAAGGTCGG TAAAAACGAC ATCAGGAAGA AAGTACGTCG TCTTATGGGC
    AAATCCCACA TTGGCCTCAC TCACAGTCAG GAGATCAATG AAGTCCTGGA TCGCCTCGCA
    CATTTAAGCG GTCGGGAGCT TTCTATCAGG CCGAGTGGCA GCAGGCATAT TAAAAAACAG
    ACATTTGTTG TGCATGCTGG GACAGATACA ACTGGTGACA TATTCTTCAT GGAGGTGTGT
    GATGATTGCA TCGTCTTGCG CAGTAATATT GGCACAGTCT ATGAGCGCTG GTGGTATGAG
    AAGCTCATAA ATATGACTTA CTGTCCCAAA ACTAAAGTGT TGTGTCTGTG GAGGAGAAAC
    GGCCAGGAGA CGCAGCTAAA CAAGTTTTAC ACTAAGAAGT GTCGGGAATT GTACTACTGT
    GTTAAAGACA GCATGGAGCG TGCTGCTGCT CGACAACAAA GCATCAAACC AGGTCCAGAG
    CTTGGAGGCG AGTTTCCGGT TCAGGACATG AAGACAGGGG AGGGTGGTCT CCTCCAAGTC
    ACACTGGAAG GCATCAACCT CAAATTTATG CACAGCCAGG AGCGGAAGGT TTTCATAGAG
    CTGAATCACA TTAAAAAGTG CAATACAGTG AAAGGAGTCT TTGTCCTGGA GGAGTTTGTT
    CCTGAAACTA AAGAAGTGGT GATCCATAAG TACAAGACTC CTATGGCACA TCAGATCTGT
    TACTCGGTGC TCTGCCTCTT CTCCTACATG GCGGCGGTCA AGGGGAAAGA GTCAGAGGGC
    AAACCCAAGA TGCTGTCCCC TCGTCCACTA GCGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48926
    Clone ID Related Accession (Same CDS sequence) XM_009303277.2
    Accession Version XM_009303277.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5016bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product MAP kinase-activating death domain protein isoform X14
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303277.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGTG TCTGGTAAGG
    TTGACTCTGA TCACCCAAAA GCAGATCTTC GCCTCCGATT CTAAGGCTCT GGCTAGCATG
    AGTCTAAACA CACAGCCTAT TCTGAACCTG GAGAAGTTTC AGGATGGACA GGAGTTGTCT
    TTATTGCCCC CCGGTCGTGA CAAAGCTTCC CCGTCCTCCA CTGAGTTCAA CCCACTCATC
    TACGGCAATG ATGTGGACTC AGTTGATGTT GCCACAAGGG TGGCAATGGT GAGGTTCTTC
    AACTCTCCAA ACGTGCTGCA AGGGTTCCAG ATGCACACAC GCACTCTCCG TCTCTTCCCT
    CGTCCTGTGG TGGCCTTTCA GTCCACATCT TTCCTGGCTT CTAGGCCACG ACGAAATGGA
    TTCACAGAAA AGCTCTCCCA CACTCAGGCA GTGGAGTACT ATGGAGAATG GGCTCTCAAC
    CCCACCAACC TGGCTTTCCA AAGGATTCAT AACAATGTGT ATGACCCCTC TCTAATAGGC
    GATAAGCCTA AGTGGTATGC CCACCAGTTA CAGCCAGTAT TCTACCGCGT GTATGATGGA
    AACTCTCATT TAGCGGAGGC CTTGAGTGGA CCCCTGCAGG ATGAGACCAA TGATTCAGAC
    CCAACAGACG ACAGTGGCAG CGACAGTGAA GCATATGATG ACTCCAGCTC CTCCTACTCA
    TCTCTTGGAG ATTTTGTGAA TGAGATGATC AAAGGGGACA TACAGGGAGA CACACCAAAT
    GTTGATCCCC TGACCCATGC TGCACTGGGA GATGCAGATG AGGTTGAGAT CCATGACTTT
    CACGAGTATA AGGGGAACAG CGGTGAACCA GAGCCAGAAG GCCCCACAGA AGCAGCGGAC
    AGTCAGCCCT TGCGGTCCAG CTCTAGCACC ACAGCCAGTT CCAGCCCCAG TACAGTCATA
    CAGGGAGTCA ACCATGAGCA GAAGGAACCT GTTGAGGTGG AGCCCATAGC AAATGTGACT
    TACCCAAGCT CTGCCCCAGT ATTGGGTCCT CCTCCTTTTA CAAGACCCAC CCCTGATCCA
    ACCCTTGTTG ACCCTGCCAA TAAGAAGCGA GAATATGACA ACCCCTATTT TGAGCCCCAG
    TACGGTTTCC CTACAGAGGA AGATGCTGAG ACTGATGAGC AGGAGGAGAG TTACACACCA
    CGATTTAACC AGAATCTCAA TGGCAATAAG CCTTCTCGTC CACTGAGACC CAGCAGCCTG
    AAGCTTCCGG GTGAATCTGA CGGAGAGGGA GACTCCAGAA ATAGCTCTCC TAACTCAACC
    ATCTCCAACA ATAGCAGTGA TGGCTTTGGG GGACTCATGT CCTTTGCCAG TAACTTGTAT
    AAAAACCATG GCACCAGTTT CAGTCTGTCC AGCCTGGCTC TGCCTAATAA AGCGAGAGAG
    AAGAACACGC CGTTTCCCAG CCTCAAAGGT GCCCGAGCCC CCAGAGCACT GGTAGATCAG
    AAATCCTCTG TTATTAAGCA CAGCCCTACT GTGAAGAGAG AGTCTCCGTC TCCTCAAGGC
    AGAGCCAACA ATACCAGTGA GAACCAGCAG TTTCTAAAGG AGGTGGTCCA GAGCGTGCTG
    GAGGGGCAGG GTGTGGGCTG GCTTAACATG AAGAAGGTGC GTCGCCTGCT AGAGAATGAA
    CAGCTCAGAG TCTTTGTGCT CAGCAAACTC AACCGTGCGG TCCAGTCAGA GGAGGACGCC
    CAGCAGGAAG TTATAAGAGA TGTGGAGATA AATAGAAAGG TCTACAAAGG CATGCTGGAT
    ATTTTGAAGT GCACGGTGTC CAGTTTGGAG CATTCATACA CTAATGCTGG CTTAGGGGGC
    ATGGCCAGTG TCTTCAGTCT GCTAGAGATA GCACGCACCC ACTACCAAAC CAAAGATCCA
    GAGAAGCGGA AACGGAGCCC CACCGAAGGG CTTAGCAGTC CAGGCAGTAA GGAGAGCCCA
    TCGGGCCGAA TGGAGAGTGC CAGGGCGGCA GGGGTGCTTC TGGTACCCCG TATCCAATTG
    CCGCCACCCT CCTCTGGGAA ATCATCACGC CAGTTTGATA CCCGGAGTCT GAACGAGGAG
    AATTTTATTG CCTCCATTGA ATTGTGGAGC AAGCACCAGG ATAACAGAAA GCAAAAAGCT
    TTGGAAAAAG AACAGAGTGG TTTAAATAAA CGGAAATCAG GAGCGGATGG AGTAAAACAA
    CGTGTAGAAG GAGGAGACAC TGAGGAGAAG AAATCACAGA TCAGTGCAGA CAGTGGCCTC
    AGTGTGACAT CTGGCTCACA GAAGAGTGAT ACGGATTCCC TGGCGAGCTC TGAACCTCCT
    GCTCTGACCA GAAGCACCAG CCAGGACTCA GAGGCCAGCA CAGTGGTAAG TAACAGCTCA
    GGTGAGACGC TCGGTGCAGA CAGTGACCTG AGCAGCACTG CTGGAGATGG CCTGACAGGA
    AGACATGGTC AGCATCTCAA CCTGTCCCGT GGCACACTGT CTGACAGTGA GATTGAGACC
    AACCCTGCAA CCAGCTCAGT GTTTGGCAAG ACACACAAGC TGAAGCCAGG AGTGAAGGAG
    CCTGTTGGTG TTAATAAAGC AGCACCTGCT CCACCCGTTG AGGATGTTAG CATGAGGATC
    TATCTGTGTG AGGGGCTTCT GGGCCGGGAT AAAAGCTCAG TTTGGGACCA GTTGGAGGAT
    GCTGCAATGG AAACCTTTTC TCTGAGTAAA GAGCGCTCAA CTCTGTGGGA TCAGATGCAG
    TTCTGGGAGG ACGCCTTCCT GGATGCCGTA ATGCTGGAAC GGGAAGGAAT GGGAATGGAT
    CAGGGACCAC AAGAAATGAT TGACAGGTAT CTTTCTCTGG GAGATCATGA CCGCAAGAGA
    CTGGAGGATG ATGAGGACAG ACTGCTGGCC ACGCTTCTGC ACAATATGAT TGCTTACATG
    CTTATGATGA AGGTCGGTAA AAACGACATC AGGAAGAAAG TACGTCGTCT TATGGGCAAA
    TCCCACATTG GCCTCACTCA CAGTCAGGAG ATCAATGAAG TCCTGGATCG CCTCGCACAT
    TTAAGCGGTC GGGAGCTTTC TATCAGGCCG AGTGGCAGCA GGCATATTAA AAAACAGACA
    TTTGTTGTGC ATGCTGGGAC AGATACAACT GGTGACATAT TCTTCATGGA GGTGTGTGAT
    GATTGCATCG TCTTGCGCAG TAATATTGGC ACAGTCTATG AGCGCTGGTG GTATGAGAAG
    CTCATAAATA TGACTTACTG TCCCAAAACT AAAGTGTTGT GTCTGTGGAG GAGAAACGGC
    CAGGAGACGC AGCTAAACAA GTTTTACACT AAGAAGTGTC GGGAATTGTA CTACTGTGTT
    AAAGACAGCA TGGAGCGTGC TGCTGCTCGA CAACAAAGCA TCAAACCAGG TCCAGAGCTT
    GGAGGCGAGT TTCCGGTTCA GGACATGAAG ACAGGGGAGG GTGGTCTCCT CCAAGTCACA
    CTGGAAGGCA TCAACCTCAA ATTTATGCAC AGCCAGGAGC GGAAGGTTTT CATAGAGCTG
    AATCACATTA AAAAGTGCAA TACAGTGAAA GGAGTCTTTG TCCTGGAGGA GTTTGTTCCT
    GAAACTAAAG AAGTGGTGAT CCATAAGTAC AAGACTCCTA TGGCACATCA GATCTGTTAC
    TCGGTGCTCT GCCTCTTCTC CTACATGGCG GCGGTCAAGG GGAAAGAGTC AGAGGGCAAA
    CCCAAGATGC TGTCCCCTCG TCCACTAGCG AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301552.1
    CDS435..5450
    Translation

    Target ORF information:

    RefSeq Version XM_009303277.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303277.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGTG TCTGGTAAGG
    TTGACTCTGA TCACCCAAAA GCAGATCTTC GCCTCCGATT CTAAGGCTCT GGCTAGCATG
    AGTCTAAACA CACAGCCTAT TCTGAACCTG GAGAAGTTTC AGGATGGACA GGAGTTGTCT
    TTATTGCCCC CCGGTCGTGA CAAAGCTTCC CCGTCCTCCA CTGAGTTCAA CCCACTCATC
    TACGGCAATG ATGTGGACTC AGTTGATGTT GCCACAAGGG TGGCAATGGT GAGGTTCTTC
    AACTCTCCAA ACGTGCTGCA AGGGTTCCAG ATGCACACAC GCACTCTCCG TCTCTTCCCT
    CGTCCTGTGG TGGCCTTTCA GTCCACATCT TTCCTGGCTT CTAGGCCACG ACGAAATGGA
    TTCACAGAAA AGCTCTCCCA CACTCAGGCA GTGGAGTACT ATGGAGAATG GGCTCTCAAC
    CCCACCAACC TGGCTTTCCA AAGGATTCAT AACAATGTGT ATGACCCCTC TCTAATAGGC
    GATAAGCCTA AGTGGTATGC CCACCAGTTA CAGCCAGTAT TCTACCGCGT GTATGATGGA
    AACTCTCATT TAGCGGAGGC CTTGAGTGGA CCCCTGCAGG ATGAGACCAA TGATTCAGAC
    CCAACAGACG ACAGTGGCAG CGACAGTGAA GCATATGATG ACTCCAGCTC CTCCTACTCA
    TCTCTTGGAG ATTTTGTGAA TGAGATGATC AAAGGGGACA TACAGGGAGA CACACCAAAT
    GTTGATCCCC TGACCCATGC TGCACTGGGA GATGCAGATG AGGTTGAGAT CCATGACTTT
    CACGAGTATA AGGGGAACAG CGGTGAACCA GAGCCAGAAG GCCCCACAGA AGCAGCGGAC
    AGTCAGCCCT TGCGGTCCAG CTCTAGCACC ACAGCCAGTT CCAGCCCCAG TACAGTCATA
    CAGGGAGTCA ACCATGAGCA GAAGGAACCT GTTGAGGTGG AGCCCATAGC AAATGTGACT
    TACCCAAGCT CTGCCCCAGT ATTGGGTCCT CCTCCTTTTA CAAGACCCAC CCCTGATCCA
    ACCCTTGTTG ACCCTGCCAA TAAGAAGCGA GAATATGACA ACCCCTATTT TGAGCCCCAG
    TACGGTTTCC CTACAGAGGA AGATGCTGAG ACTGATGAGC AGGAGGAGAG TTACACACCA
    CGATTTAACC AGAATCTCAA TGGCAATAAG CCTTCTCGTC CACTGAGACC CAGCAGCCTG
    AAGCTTCCGG GTGAATCTGA CGGAGAGGGA GACTCCAGAA ATAGCTCTCC TAACTCAACC
    ATCTCCAACA ATAGCAGTGA TGGCTTTGGG GGACTCATGT CCTTTGCCAG TAACTTGTAT
    AAAAACCATG GCACCAGTTT CAGTCTGTCC AGCCTGGCTC TGCCTAATAA AGCGAGAGAG
    AAGAACACGC CGTTTCCCAG CCTCAAAGGT GCCCGAGCCC CCAGAGCACT GGTAGATCAG
    AAATCCTCTG TTATTAAGCA CAGCCCTACT GTGAAGAGAG AGTCTCCGTC TCCTCAAGGC
    AGAGCCAACA ATACCAGTGA GAACCAGCAG TTTCTAAAGG AGGTGGTCCA GAGCGTGCTG
    GAGGGGCAGG GTGTGGGCTG GCTTAACATG AAGAAGGTGC GTCGCCTGCT AGAGAATGAA
    CAGCTCAGAG TCTTTGTGCT CAGCAAACTC AACCGTGCGG TCCAGTCAGA GGAGGACGCC
    CAGCAGGAAG TTATAAGAGA TGTGGAGATA AATAGAAAGG TCTACAAAGG CATGCTGGAT
    ATTTTGAAGT GCACGGTGTC CAGTTTGGAG CATTCATACA CTAATGCTGG CTTAGGGGGC
    ATGGCCAGTG TCTTCAGTCT GCTAGAGATA GCACGCACCC ACTACCAAAC CAAAGATCCA
    GAGAAGCGGA AACGGAGCCC CACCGAAGGG CTTAGCAGTC CAGGCAGTAA GGAGAGCCCA
    TCGGGCCGAA TGGAGAGTGC CAGGGCGGCA GGGGTGCTTC TGGTACCCCG TATCCAATTG
    CCGCCACCCT CCTCTGGGAA ATCATCACGC CAGTTTGATA CCCGGAGTCT GAACGAGGAG
    AATTTTATTG CCTCCATTGA ATTGTGGAGC AAGCACCAGG ATAACAGAAA GCAAAAAGCT
    TTGGAAAAAG AACAGAGTGG TTTAAATAAA CGGAAATCAG GAGCGGATGG AGTAAAACAA
    CGTGTAGAAG GAGGAGACAC TGAGGAGAAG AAATCACAGA TCAGTGCAGA CAGTGGCCTC
    AGTGTGACAT CTGGCTCACA GAAGAGTGAT ACGGATTCCC TGGCGAGCTC TGAACCTCCT
    GCTCTGACCA GAAGCACCAG CCAGGACTCA GAGGCCAGCA CAGTGGTAAG TAACAGCTCA
    GGTGAGACGC TCGGTGCAGA CAGTGACCTG AGCAGCACTG CTGGAGATGG CCTGACAGGA
    AGACATGGTC AGCATCTCAA CCTGTCCCGT GGCACACTGT CTGACAGTGA GATTGAGACC
    AACCCTGCAA CCAGCTCAGT GTTTGGCAAG ACACACAAGC TGAAGCCAGG AGTGAAGGAG
    CCTGTTGGTG TTAATAAAGC AGCACCTGCT CCACCCGTTG AGGATGTTAG CATGAGGATC
    TATCTGTGTG AGGGGCTTCT GGGCCGGGAT AAAAGCTCAG TTTGGGACCA GTTGGAGGAT
    GCTGCAATGG AAACCTTTTC TCTGAGTAAA GAGCGCTCAA CTCTGTGGGA TCAGATGCAG
    TTCTGGGAGG ACGCCTTCCT GGATGCCGTA ATGCTGGAAC GGGAAGGAAT GGGAATGGAT
    CAGGGACCAC AAGAAATGAT TGACAGGTAT CTTTCTCTGG GAGATCATGA CCGCAAGAGA
    CTGGAGGATG ATGAGGACAG ACTGCTGGCC ACGCTTCTGC ACAATATGAT TGCTTACATG
    CTTATGATGA AGGTCGGTAA AAACGACATC AGGAAGAAAG TACGTCGTCT TATGGGCAAA
    TCCCACATTG GCCTCACTCA CAGTCAGGAG ATCAATGAAG TCCTGGATCG CCTCGCACAT
    TTAAGCGGTC GGGAGCTTTC TATCAGGCCG AGTGGCAGCA GGCATATTAA AAAACAGACA
    TTTGTTGTGC ATGCTGGGAC AGATACAACT GGTGACATAT TCTTCATGGA GGTGTGTGAT
    GATTGCATCG TCTTGCGCAG TAATATTGGC ACAGTCTATG AGCGCTGGTG GTATGAGAAG
    CTCATAAATA TGACTTACTG TCCCAAAACT AAAGTGTTGT GTCTGTGGAG GAGAAACGGC
    CAGGAGACGC AGCTAAACAA GTTTTACACT AAGAAGTGTC GGGAATTGTA CTACTGTGTT
    AAAGACAGCA TGGAGCGTGC TGCTGCTCGA CAACAAAGCA TCAAACCAGG TCCAGAGCTT
    GGAGGCGAGT TTCCGGTTCA GGACATGAAG ACAGGGGAGG GTGGTCTCCT CCAAGTCACA
    CTGGAAGGCA TCAACCTCAA ATTTATGCAC AGCCAGGAGC GGAAGGTTTT CATAGAGCTG
    AATCACATTA AAAAGTGCAA TACAGTGAAA GGAGTCTTTG TCCTGGAGGA GTTTGTTCCT
    GAAACTAAAG AAGTGGTGAT CCATAAGTAC AAGACTCCTA TGGCACATCA GATCTGTTAC
    TCGGTGCTCT GCCTCTTCTC CTACATGGCG GCGGTCAAGG GGAAAGAGTC AGAGGGCAAA
    CCCAAGATGC TGTCCCCTCG TCCACTAGCG AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48927
    Clone ID Related Accession (Same CDS sequence) XM_009303278.3 , XM_009303278.2
    Accession Version XM_009303278.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4959bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303278.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGAATTGTGG AGCAAGCACC AGGATAACAG AAAGCAAAAA
    GCTTTGGAAA AAGAACAGAG TGGTTTAAAT AAACGGAAAT CAGGAGCGGA TGGAGTAAAA
    CAACGTGTAG AAGGAGGAGA CACTGAGGAG AAGAAATCAC AGATCAGTGC AGACAGTGGC
    CTCAGTGTGA CATCTGGCTC ACAGAAGAGT GATACGGATT CCCTGGCGAG CTCTGAACCT
    CCTGCTCTGA CCAGAAGCAC CAGCCAGGAC TCAGAGGCCA GCACAGTGGT AAGTAACAGC
    TCAGGTGAGA CGCTCGGTGC AGACAGTGAC CTGAGCAGCA CTGCTGGAGA TGGCCTGACA
    GGAAGACATG GTCAGCATCT CAACCTGTCC CGTGGCACAC TGTCTGACAG TGAGATTGAG
    ACCAACCCTG CAACCAGCTC AGTGTTTGGC AAGACACACA AGCTGAAGCC AGGAGTGAAG
    GAGCCTGTTG GTGTTAATAA AGCAGCACCT GCTCCACCCG TTGAGGATGT TAGCATGAGG
    ATCTATCTGT GTGAGGGGCT TCTGGGCCGG GATAAAAGCT CAGTTTGGGA CCAGTTGGAG
    GATGCTGCAA TGGAAACCTT TTCTCTGAGT AAAGAGCGCT CAACTCTGTG GGATCAGATG
    CAGTTCTGGG AGGACGCCTT CCTGGATGCC GTAATGCTGG AACGGGAAGG AATGGGAATG
    GATCAGGGAC CACAAGAAAT GATTGACAGG TATCTTTCTC TGGGAGATCA TGACCGCAAG
    AGACTGGAGG ATGATGAGGA CAGACTGCTG GCCACGCTTC TGCACAATAT GATTGCTTAC
    ATGCTTATGA TGAAGGTCGG TAAAAACGAC ATCAGGAAGA AAGTACGTCG TCTTATGGGC
    AAATCCCACA TTGGCCTCAC TCACAGTCAG GAGATCAATG AAGTCCTGGA TCGCCTCGCA
    CATTTAAGCG GTCGGGAGCT TTCTATCAGG CCGAGTGGCA GCAGGCATAT TAAAAAACAG
    ACATTTGTTG TGCATGCTGG GACAGATACA ACTGGTGACA TATTCTTCAT GGAGGTGTGT
    GATGATTGCA TCGTCTTGCG CAGTAATATT GGCACAGTCT ATGAGCGCTG GTGGTATGAG
    AAGCTCATAA ATATGACTTA CTGTCCCAAA ACTAAAGTGT TGTGTCTGTG GAGGAGAAAC
    GGCCAGGAGA CGCAGCTAAA CAAGTTTTAC ACTAAGAAGT GTCGGGAATT GTACTACTGT
    GTTAAAGACA GCATGGAGCG TGCTGCTGCT CGACAACAAA GCATCAAACC AGGTCCAGAG
    CTTGGAGGCG AGTTTCCGGT TCAGGACATG AAGACAGGGG AGGGTGGTCT CCTCCAAGTC
    ACACTGGAAG GCATCAACCT CAAATTTATG CACAGCCAGG AGCGGAAGGT TTTCATAGAG
    CTGAATCACA TTAAAAAGTG CAATACAGTG AAAGGAGTCT TTGTCCTGGA GGAGTTTGTT
    CCTGAAACTA AAGAAGTGGT GATCCATAAG TACAAGACTC CTATGGCACA TCAGATCTGT
    TACTCGGTGC TCTGCCTCTT CTCCTACATG GCGGCGGTCA AGGGGAAAGA GTCAGAGGGC
    AAACCCAAGA TGCTGTCCCC TCGTCCACTA GCGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301553.1
    CDS432..5390
    Translation

    Target ORF information:

    RefSeq Version XM_009303278.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303278.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGAATTGTGG AGCAAGCACC AGGATAACAG AAAGCAAAAA
    GCTTTGGAAA AAGAACAGAG TGGTTTAAAT AAACGGAAAT CAGGAGCGGA TGGAGTAAAA
    CAACGTGTAG AAGGAGGAGA CACTGAGGAG AAGAAATCAC AGATCAGTGC AGACAGTGGC
    CTCAGTGTGA CATCTGGCTC ACAGAAGAGT GATACGGATT CCCTGGCGAG CTCTGAACCT
    CCTGCTCTGA CCAGAAGCAC CAGCCAGGAC TCAGAGGCCA GCACAGTGGT AAGTAACAGC
    TCAGGTGAGA CGCTCGGTGC AGACAGTGAC CTGAGCAGCA CTGCTGGAGA TGGCCTGACA
    GGAAGACATG GTCAGCATCT CAACCTGTCC CGTGGCACAC TGTCTGACAG TGAGATTGAG
    ACCAACCCTG CAACCAGCTC AGTGTTTGGC AAGACACACA AGCTGAAGCC AGGAGTGAAG
    GAGCCTGTTG GTGTTAATAA AGCAGCACCT GCTCCACCCG TTGAGGATGT TAGCATGAGG
    ATCTATCTGT GTGAGGGGCT TCTGGGCCGG GATAAAAGCT CAGTTTGGGA CCAGTTGGAG
    GATGCTGCAA TGGAAACCTT TTCTCTGAGT AAAGAGCGCT CAACTCTGTG GGATCAGATG
    CAGTTCTGGG AGGACGCCTT CCTGGATGCC GTAATGCTGG AACGGGAAGG AATGGGAATG
    GATCAGGGAC CACAAGAAAT GATTGACAGG TATCTTTCTC TGGGAGATCA TGACCGCAAG
    AGACTGGAGG ATGATGAGGA CAGACTGCTG GCCACGCTTC TGCACAATAT GATTGCTTAC
    ATGCTTATGA TGAAGGTCGG TAAAAACGAC ATCAGGAAGA AAGTACGTCG TCTTATGGGC
    AAATCCCACA TTGGCCTCAC TCACAGTCAG GAGATCAATG AAGTCCTGGA TCGCCTCGCA
    CATTTAAGCG GTCGGGAGCT TTCTATCAGG CCGAGTGGCA GCAGGCATAT TAAAAAACAG
    ACATTTGTTG TGCATGCTGG GACAGATACA ACTGGTGACA TATTCTTCAT GGAGGTGTGT
    GATGATTGCA TCGTCTTGCG CAGTAATATT GGCACAGTCT ATGAGCGCTG GTGGTATGAG
    AAGCTCATAA ATATGACTTA CTGTCCCAAA ACTAAAGTGT TGTGTCTGTG GAGGAGAAAC
    GGCCAGGAGA CGCAGCTAAA CAAGTTTTAC ACTAAGAAGT GTCGGGAATT GTACTACTGT
    GTTAAAGACA GCATGGAGCG TGCTGCTGCT CGACAACAAA GCATCAAACC AGGTCCAGAG
    CTTGGAGGCG AGTTTCCGGT TCAGGACATG AAGACAGGGG AGGGTGGTCT CCTCCAAGTC
    ACACTGGAAG GCATCAACCT CAAATTTATG CACAGCCAGG AGCGGAAGGT TTTCATAGAG
    CTGAATCACA TTAAAAAGTG CAATACAGTG AAAGGAGTCT TTGTCCTGGA GGAGTTTGTT
    CCTGAAACTA AAGAAGTGGT GATCCATAAG TACAAGACTC CTATGGCACA TCAGATCTGT
    TACTCGGTGC TCTGCCTCTT CTCCTACATG GCGGCGGTCA AGGGGAAAGA GTCAGAGGGC
    AAACCCAAGA TGCTGTCCCC TCGTCCACTA GCGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48927
    Clone ID Related Accession (Same CDS sequence) XM_009303278.3 , XM_009303278.2
    Accession Version XM_009303278.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4959bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product MAP kinase-activating death domain protein isoform X15
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303278.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGAATTGTGG AGCAAGCACC AGGATAACAG AAAGCAAAAA
    GCTTTGGAAA AAGAACAGAG TGGTTTAAAT AAACGGAAAT CAGGAGCGGA TGGAGTAAAA
    CAACGTGTAG AAGGAGGAGA CACTGAGGAG AAGAAATCAC AGATCAGTGC AGACAGTGGC
    CTCAGTGTGA CATCTGGCTC ACAGAAGAGT GATACGGATT CCCTGGCGAG CTCTGAACCT
    CCTGCTCTGA CCAGAAGCAC CAGCCAGGAC TCAGAGGCCA GCACAGTGGT AAGTAACAGC
    TCAGGTGAGA CGCTCGGTGC AGACAGTGAC CTGAGCAGCA CTGCTGGAGA TGGCCTGACA
    GGAAGACATG GTCAGCATCT CAACCTGTCC CGTGGCACAC TGTCTGACAG TGAGATTGAG
    ACCAACCCTG CAACCAGCTC AGTGTTTGGC AAGACACACA AGCTGAAGCC AGGAGTGAAG
    GAGCCTGTTG GTGTTAATAA AGCAGCACCT GCTCCACCCG TTGAGGATGT TAGCATGAGG
    ATCTATCTGT GTGAGGGGCT TCTGGGCCGG GATAAAAGCT CAGTTTGGGA CCAGTTGGAG
    GATGCTGCAA TGGAAACCTT TTCTCTGAGT AAAGAGCGCT CAACTCTGTG GGATCAGATG
    CAGTTCTGGG AGGACGCCTT CCTGGATGCC GTAATGCTGG AACGGGAAGG AATGGGAATG
    GATCAGGGAC CACAAGAAAT GATTGACAGG TATCTTTCTC TGGGAGATCA TGACCGCAAG
    AGACTGGAGG ATGATGAGGA CAGACTGCTG GCCACGCTTC TGCACAATAT GATTGCTTAC
    ATGCTTATGA TGAAGGTCGG TAAAAACGAC ATCAGGAAGA AAGTACGTCG TCTTATGGGC
    AAATCCCACA TTGGCCTCAC TCACAGTCAG GAGATCAATG AAGTCCTGGA TCGCCTCGCA
    CATTTAAGCG GTCGGGAGCT TTCTATCAGG CCGAGTGGCA GCAGGCATAT TAAAAAACAG
    ACATTTGTTG TGCATGCTGG GACAGATACA ACTGGTGACA TATTCTTCAT GGAGGTGTGT
    GATGATTGCA TCGTCTTGCG CAGTAATATT GGCACAGTCT ATGAGCGCTG GTGGTATGAG
    AAGCTCATAA ATATGACTTA CTGTCCCAAA ACTAAAGTGT TGTGTCTGTG GAGGAGAAAC
    GGCCAGGAGA CGCAGCTAAA CAAGTTTTAC ACTAAGAAGT GTCGGGAATT GTACTACTGT
    GTTAAAGACA GCATGGAGCG TGCTGCTGCT CGACAACAAA GCATCAAACC AGGTCCAGAG
    CTTGGAGGCG AGTTTCCGGT TCAGGACATG AAGACAGGGG AGGGTGGTCT CCTCCAAGTC
    ACACTGGAAG GCATCAACCT CAAATTTATG CACAGCCAGG AGCGGAAGGT TTTCATAGAG
    CTGAATCACA TTAAAAAGTG CAATACAGTG AAAGGAGTCT TTGTCCTGGA GGAGTTTGTT
    CCTGAAACTA AAGAAGTGGT GATCCATAAG TACAAGACTC CTATGGCACA TCAGATCTGT
    TACTCGGTGC TCTGCCTCTT CTCCTACATG GCGGCGGTCA AGGGGAAAGA GTCAGAGGGC
    AAACCCAAGA TGCTGTCCCC TCGTCCACTA GCGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301553.1
    CDS434..5392
    Translation

    Target ORF information:

    RefSeq Version XM_009303278.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303278.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGAATTGTGG AGCAAGCACC AGGATAACAG AAAGCAAAAA
    GCTTTGGAAA AAGAACAGAG TGGTTTAAAT AAACGGAAAT CAGGAGCGGA TGGAGTAAAA
    CAACGTGTAG AAGGAGGAGA CACTGAGGAG AAGAAATCAC AGATCAGTGC AGACAGTGGC
    CTCAGTGTGA CATCTGGCTC ACAGAAGAGT GATACGGATT CCCTGGCGAG CTCTGAACCT
    CCTGCTCTGA CCAGAAGCAC CAGCCAGGAC TCAGAGGCCA GCACAGTGGT AAGTAACAGC
    TCAGGTGAGA CGCTCGGTGC AGACAGTGAC CTGAGCAGCA CTGCTGGAGA TGGCCTGACA
    GGAAGACATG GTCAGCATCT CAACCTGTCC CGTGGCACAC TGTCTGACAG TGAGATTGAG
    ACCAACCCTG CAACCAGCTC AGTGTTTGGC AAGACACACA AGCTGAAGCC AGGAGTGAAG
    GAGCCTGTTG GTGTTAATAA AGCAGCACCT GCTCCACCCG TTGAGGATGT TAGCATGAGG
    ATCTATCTGT GTGAGGGGCT TCTGGGCCGG GATAAAAGCT CAGTTTGGGA CCAGTTGGAG
    GATGCTGCAA TGGAAACCTT TTCTCTGAGT AAAGAGCGCT CAACTCTGTG GGATCAGATG
    CAGTTCTGGG AGGACGCCTT CCTGGATGCC GTAATGCTGG AACGGGAAGG AATGGGAATG
    GATCAGGGAC CACAAGAAAT GATTGACAGG TATCTTTCTC TGGGAGATCA TGACCGCAAG
    AGACTGGAGG ATGATGAGGA CAGACTGCTG GCCACGCTTC TGCACAATAT GATTGCTTAC
    ATGCTTATGA TGAAGGTCGG TAAAAACGAC ATCAGGAAGA AAGTACGTCG TCTTATGGGC
    AAATCCCACA TTGGCCTCAC TCACAGTCAG GAGATCAATG AAGTCCTGGA TCGCCTCGCA
    CATTTAAGCG GTCGGGAGCT TTCTATCAGG CCGAGTGGCA GCAGGCATAT TAAAAAACAG
    ACATTTGTTG TGCATGCTGG GACAGATACA ACTGGTGACA TATTCTTCAT GGAGGTGTGT
    GATGATTGCA TCGTCTTGCG CAGTAATATT GGCACAGTCT ATGAGCGCTG GTGGTATGAG
    AAGCTCATAA ATATGACTTA CTGTCCCAAA ACTAAAGTGT TGTGTCTGTG GAGGAGAAAC
    GGCCAGGAGA CGCAGCTAAA CAAGTTTTAC ACTAAGAAGT GTCGGGAATT GTACTACTGT
    GTTAAAGACA GCATGGAGCG TGCTGCTGCT CGACAACAAA GCATCAAACC AGGTCCAGAG
    CTTGGAGGCG AGTTTCCGGT TCAGGACATG AAGACAGGGG AGGGTGGTCT CCTCCAAGTC
    ACACTGGAAG GCATCAACCT CAAATTTATG CACAGCCAGG AGCGGAAGGT TTTCATAGAG
    CTGAATCACA TTAAAAAGTG CAATACAGTG AAAGGAGTCT TTGTCCTGGA GGAGTTTGTT
    CCTGAAACTA AAGAAGTGGT GATCCATAAG TACAAGACTC CTATGGCACA TCAGATCTGT
    TACTCGGTGC TCTGCCTCTT CTCCTACATG GCGGCGGTCA AGGGGAAAGA GTCAGAGGGC
    AAACCCAAGA TGCTGTCCCC TCGTCCACTA GCGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48928
    Clone ID Related Accession (Same CDS sequence) XM_009303279.2
    Accession Version XM_009303279.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4935bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product MAP kinase-activating death domain protein isoform X16
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303279.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGTG TCTGGTAAGG
    TTGACTCTGA TCACCCAAAA GCAGATCTTC GCCTCCGATT CTAAGGCTCT GGCTAGCATG
    AGTCTAAACA CACAGCCTAT TCTGAACCTG GAGAAGTTTC AGGATGGACA GGAGTTGTCT
    TTATTGCCCC CCGGTCGTGA CAAAGCTTCC CCGTCCTCCA CTGAGTTCAA CCCACTCATC
    TACGGCAATG ATGTGGACTC AGTTGATGTT GCCACAAGGG TGGCAATGGT GAGGTTCTTC
    AACTCTCCAA ACGTGCTGCA AGGGTTCCAG ATGCACACAC GCACTCTCCG TCTCTTCCCT
    CGTCCTGTGG TGGCCTTTCA GTCCACATCT TTCCTGGCTT CTAGGCCACG ACGAAATGGA
    TTCACAGAAA AGCTCTCCCA CACTCAGGCA GTGGAGTACT ATGGAGAATG GGCTCTCAAC
    CCCACCAACC TGGCTTTCCA AAGGATTCAT AACAATGTGT ATGACCCCTC TCTAATAGGC
    GATAAGCCTA AGTGGTATGC CCACCAGTTA CAGCCAGTAT TCTACCGCGT GTATGATGGA
    AACTCTCATT TAGCGGAGGC CTTGAGTGGA CCCCTGCAGG ATGAGACCAA TGATTCAGAC
    CCAACAGACG ACAGTGGCAG CGACAGTGAA GCATATGATG ACTCCAGCTC CTCCTACTCA
    TCTCTTGGAG ATTTTGTGAA TGAGATGATC AAAGGGGACA TACAGGGAGA CACACCAAAT
    GTTGATCCCC TGACCCATGC TGCACTGGGA GATGCAGATG AGGTTGAGAT CCATGACTTT
    CACGAGTATA AGGGGAACAG CGGTGAACCA GAGCCAGAAG GCCCCACAGA AGCAGCGGAC
    AGTCAGCCCT TGCGGTCCAG CTCTAGCACC ACAGCCAGTT CCAGCCCCAG TACAGTCATA
    CAGGGAGTCA ACCATGAGCA GAAGGAACCT GTTGAGGTGG AGCCCATAGC AAATGTGACT
    TACCCAAGCT CTGCCCCAGT ATTGGGTCCT CCTCCTTTTA CAAGACCCAC CCCTGATCCA
    ACCCTTGTTG ACCCTGCCAA TAAGAAGCGA GAATATGACA ACCCCTATTT TGAGCCCCAG
    TACGGTTTCC CTACAGAGGA AGATGCTGAG ACTGATGAGC AGGAGGAGAG TTACACACCA
    CGATTTAACC AGAATCTCAA TGGCAATAAG CCTTCTCGTC CACTGAGACC CAGCAGCCTG
    AAGCTTCCGG GTGAATCTGA CGGAGAGGGA GACTCCAGAA ATAGCTCTCC TAACTCAACC
    ATCTCCAACA ATAGCAGTGA TGGCTTTGGG GGACTCATGT CCTTTGCCAG TAACTTGTAT
    AAAAACCATG GCACCAGTTT CAGTCTGTCC AGCCTGGCTC TGCCTAATAA AGCGAGAGAG
    AAGAACACGC CGTTTCCCAG CCTCAAAGGT GCCCGAGCCC CCAGAGCACT GGTAGATCAG
    AAATCCTCTG TTATTAAGCA CAGCCCTACT GTGAAGAGAG AGTCTCCGTC TCCTCAAGGC
    AGAGCCAACA ATACCAGTGA GAACCAGCAG TTTCTAAAGG AGGTGGTCCA GAGCGTGCTG
    GAGGGGCAGG GTGTGGGCTG GCTTAACATG AAGAAGGTGC GTCGCCTGCT AGAGAATGAA
    CAGCTCAGAG TCTTTGTGCT CAGCAAACTC AACCGTGCGG TCCAGTCAGA GGAGGACGCC
    CAGCAGGAAG TTATAAGAGA TGTGGAGATA AATAGAAAGG TCTACAAAGG CATGCTGGAT
    ATTTTGAAGT GCACGGTGTC CAGTTTGGAG CATTCATACA CTAATGCTGG CTTAGGGGGC
    ATGGCCAGTG TCTTCAGTCT GCTAGAGATA GCACGCACCC ACTACCAAAC CAAAGATCCA
    GAGAAGCGGA AACGGAGCCC CACCGAAGGG CTTAGCAGTC CAGGCAGTAA GGAGAGCCCA
    TCGGGCCGAA TGGAGAGTGC CAGGGCGGCA GGGGTGCTTC TGGTACCCCG TATCCAATTG
    CCGCCACCCT CCTCTGGGAA ATCATCACGC CAGTTTGATA CCCGGAGTCT GAACGAGGAG
    AATTTTATTG CCTCCATTGG AGCGGATGGA GTAAAACAAC GTGTAGAAGG AGGAGACACT
    GAGGAGAAGA AATCACAGAT CAGTGCAGAC AGTGGCCTCA GTGTGACATC TGGCTCACAG
    AAGAGTGATA CGGATTCCCT GGCGAGCTCT GAACCTCCTG CTCTGACCAG AAGCACCAGC
    CAGGACTCAG AGGCCAGCAC AGTGGTAAGT AACAGCTCAG GTGAGACGCT CGGTGCAGAC
    AGTGACCTGA GCAGCACTGC TGGAGATGGC CTGACAGGAA GACATGGTCA GCATCTCAAC
    CTGTCCCGTG GCACACTGTC TGACAGTGAG ATTGAGACCA ACCCTGCAAC CAGCTCAGTG
    TTTGGCAAGA CACACAAGCT GAAGCCAGGA GTGAAGGAGC CTGTTGGTGT TAATAAAGCA
    GCACCTGCTC CACCCGTTGA GGATGTTAGC ATGAGGATCT ATCTGTGTGA GGGGCTTCTG
    GGCCGGGATA AAAGCTCAGT TTGGGACCAG TTGGAGGATG CTGCAATGGA AACCTTTTCT
    CTGAGTAAAG AGCGCTCAAC TCTGTGGGAT CAGATGCAGT TCTGGGAGGA CGCCTTCCTG
    GATGCCGTAA TGCTGGAACG GGAAGGAATG GGAATGGATC AGGGACCACA AGAAATGATT
    GACAGGTATC TTTCTCTGGG AGATCATGAC CGCAAGAGAC TGGAGGATGA TGAGGACAGA
    CTGCTGGCCA CGCTTCTGCA CAATATGATT GCTTACATGC TTATGATGAA GGTCGGTAAA
    AACGACATCA GGAAGAAAGT ACGTCGTCTT ATGGGCAAAT CCCACATTGG CCTCACTCAC
    AGTCAGGAGA TCAATGAAGT CCTGGATCGC CTCGCACATT TAAGCGGTCG GGAGCTTTCT
    ATCAGGCCGA GTGGCAGCAG GCATATTAAA AAACAGACAT TTGTTGTGCA TGCTGGGACA
    GATACAACTG GTGACATATT CTTCATGGAG GTGTGTGATG ATTGCATCGT CTTGCGCAGT
    AATATTGGCA CAGTCTATGA GCGCTGGTGG TATGAGAAGC TCATAAATAT GACTTACTGT
    CCCAAAACTA AAGTGTTGTG TCTGTGGAGG AGAAACGGCC AGGAGACGCA GCTAAACAAG
    TTTTACACTA AGAAGTGTCG GGAATTGTAC TACTGTGTTA AAGACAGCAT GGAGCGTGCT
    GCTGCTCGAC AACAAAGCAT CAAACCAGGT CCAGAGCTTG GAGGCGAGTT TCCGGTTCAG
    GACATGAAGA CAGGGGAGGG TGGTCTCCTC CAAGTCACAC TGGAAGGCAT CAACCTCAAA
    TTTATGCACA GCCAGGAGCG GAAGGTTTTC ATAGAGCTGA ATCACATTAA AAAGTGCAAT
    ACAGTGAAAG GAGTCTTTGT CCTGGAGGAG TTTGTTCCTG AAACTAAAGA AGTGGTGATC
    CATAAGTACA AGACTCCTAT GGCACATCAG ATCTGTTACT CGGTGCTCTG CCTCTTCTCC
    TACATGGCGG CGGTCAAGGG GAAAGAGTCA GAGGGCAAAC CCAAGATGCT GTCCCCTCGT
    CCACTAGCGA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301554.1
    CDS435..5369
    Translation

    Target ORF information:

    RefSeq Version XM_009303279.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303279.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGTG TCTGGTAAGG
    TTGACTCTGA TCACCCAAAA GCAGATCTTC GCCTCCGATT CTAAGGCTCT GGCTAGCATG
    AGTCTAAACA CACAGCCTAT TCTGAACCTG GAGAAGTTTC AGGATGGACA GGAGTTGTCT
    TTATTGCCCC CCGGTCGTGA CAAAGCTTCC CCGTCCTCCA CTGAGTTCAA CCCACTCATC
    TACGGCAATG ATGTGGACTC AGTTGATGTT GCCACAAGGG TGGCAATGGT GAGGTTCTTC
    AACTCTCCAA ACGTGCTGCA AGGGTTCCAG ATGCACACAC GCACTCTCCG TCTCTTCCCT
    CGTCCTGTGG TGGCCTTTCA GTCCACATCT TTCCTGGCTT CTAGGCCACG ACGAAATGGA
    TTCACAGAAA AGCTCTCCCA CACTCAGGCA GTGGAGTACT ATGGAGAATG GGCTCTCAAC
    CCCACCAACC TGGCTTTCCA AAGGATTCAT AACAATGTGT ATGACCCCTC TCTAATAGGC
    GATAAGCCTA AGTGGTATGC CCACCAGTTA CAGCCAGTAT TCTACCGCGT GTATGATGGA
    AACTCTCATT TAGCGGAGGC CTTGAGTGGA CCCCTGCAGG ATGAGACCAA TGATTCAGAC
    CCAACAGACG ACAGTGGCAG CGACAGTGAA GCATATGATG ACTCCAGCTC CTCCTACTCA
    TCTCTTGGAG ATTTTGTGAA TGAGATGATC AAAGGGGACA TACAGGGAGA CACACCAAAT
    GTTGATCCCC TGACCCATGC TGCACTGGGA GATGCAGATG AGGTTGAGAT CCATGACTTT
    CACGAGTATA AGGGGAACAG CGGTGAACCA GAGCCAGAAG GCCCCACAGA AGCAGCGGAC
    AGTCAGCCCT TGCGGTCCAG CTCTAGCACC ACAGCCAGTT CCAGCCCCAG TACAGTCATA
    CAGGGAGTCA ACCATGAGCA GAAGGAACCT GTTGAGGTGG AGCCCATAGC AAATGTGACT
    TACCCAAGCT CTGCCCCAGT ATTGGGTCCT CCTCCTTTTA CAAGACCCAC CCCTGATCCA
    ACCCTTGTTG ACCCTGCCAA TAAGAAGCGA GAATATGACA ACCCCTATTT TGAGCCCCAG
    TACGGTTTCC CTACAGAGGA AGATGCTGAG ACTGATGAGC AGGAGGAGAG TTACACACCA
    CGATTTAACC AGAATCTCAA TGGCAATAAG CCTTCTCGTC CACTGAGACC CAGCAGCCTG
    AAGCTTCCGG GTGAATCTGA CGGAGAGGGA GACTCCAGAA ATAGCTCTCC TAACTCAACC
    ATCTCCAACA ATAGCAGTGA TGGCTTTGGG GGACTCATGT CCTTTGCCAG TAACTTGTAT
    AAAAACCATG GCACCAGTTT CAGTCTGTCC AGCCTGGCTC TGCCTAATAA AGCGAGAGAG
    AAGAACACGC CGTTTCCCAG CCTCAAAGGT GCCCGAGCCC CCAGAGCACT GGTAGATCAG
    AAATCCTCTG TTATTAAGCA CAGCCCTACT GTGAAGAGAG AGTCTCCGTC TCCTCAAGGC
    AGAGCCAACA ATACCAGTGA GAACCAGCAG TTTCTAAAGG AGGTGGTCCA GAGCGTGCTG
    GAGGGGCAGG GTGTGGGCTG GCTTAACATG AAGAAGGTGC GTCGCCTGCT AGAGAATGAA
    CAGCTCAGAG TCTTTGTGCT CAGCAAACTC AACCGTGCGG TCCAGTCAGA GGAGGACGCC
    CAGCAGGAAG TTATAAGAGA TGTGGAGATA AATAGAAAGG TCTACAAAGG CATGCTGGAT
    ATTTTGAAGT GCACGGTGTC CAGTTTGGAG CATTCATACA CTAATGCTGG CTTAGGGGGC
    ATGGCCAGTG TCTTCAGTCT GCTAGAGATA GCACGCACCC ACTACCAAAC CAAAGATCCA
    GAGAAGCGGA AACGGAGCCC CACCGAAGGG CTTAGCAGTC CAGGCAGTAA GGAGAGCCCA
    TCGGGCCGAA TGGAGAGTGC CAGGGCGGCA GGGGTGCTTC TGGTACCCCG TATCCAATTG
    CCGCCACCCT CCTCTGGGAA ATCATCACGC CAGTTTGATA CCCGGAGTCT GAACGAGGAG
    AATTTTATTG CCTCCATTGG AGCGGATGGA GTAAAACAAC GTGTAGAAGG AGGAGACACT
    GAGGAGAAGA AATCACAGAT CAGTGCAGAC AGTGGCCTCA GTGTGACATC TGGCTCACAG
    AAGAGTGATA CGGATTCCCT GGCGAGCTCT GAACCTCCTG CTCTGACCAG AAGCACCAGC
    CAGGACTCAG AGGCCAGCAC AGTGGTAAGT AACAGCTCAG GTGAGACGCT CGGTGCAGAC
    AGTGACCTGA GCAGCACTGC TGGAGATGGC CTGACAGGAA GACATGGTCA GCATCTCAAC
    CTGTCCCGTG GCACACTGTC TGACAGTGAG ATTGAGACCA ACCCTGCAAC CAGCTCAGTG
    TTTGGCAAGA CACACAAGCT GAAGCCAGGA GTGAAGGAGC CTGTTGGTGT TAATAAAGCA
    GCACCTGCTC CACCCGTTGA GGATGTTAGC ATGAGGATCT ATCTGTGTGA GGGGCTTCTG
    GGCCGGGATA AAAGCTCAGT TTGGGACCAG TTGGAGGATG CTGCAATGGA AACCTTTTCT
    CTGAGTAAAG AGCGCTCAAC TCTGTGGGAT CAGATGCAGT TCTGGGAGGA CGCCTTCCTG
    GATGCCGTAA TGCTGGAACG GGAAGGAATG GGAATGGATC AGGGACCACA AGAAATGATT
    GACAGGTATC TTTCTCTGGG AGATCATGAC CGCAAGAGAC TGGAGGATGA TGAGGACAGA
    CTGCTGGCCA CGCTTCTGCA CAATATGATT GCTTACATGC TTATGATGAA GGTCGGTAAA
    AACGACATCA GGAAGAAAGT ACGTCGTCTT ATGGGCAAAT CCCACATTGG CCTCACTCAC
    AGTCAGGAGA TCAATGAAGT CCTGGATCGC CTCGCACATT TAAGCGGTCG GGAGCTTTCT
    ATCAGGCCGA GTGGCAGCAG GCATATTAAA AAACAGACAT TTGTTGTGCA TGCTGGGACA
    GATACAACTG GTGACATATT CTTCATGGAG GTGTGTGATG ATTGCATCGT CTTGCGCAGT
    AATATTGGCA CAGTCTATGA GCGCTGGTGG TATGAGAAGC TCATAAATAT GACTTACTGT
    CCCAAAACTA AAGTGTTGTG TCTGTGGAGG AGAAACGGCC AGGAGACGCA GCTAAACAAG
    TTTTACACTA AGAAGTGTCG GGAATTGTAC TACTGTGTTA AAGACAGCAT GGAGCGTGCT
    GCTGCTCGAC AACAAAGCAT CAAACCAGGT CCAGAGCTTG GAGGCGAGTT TCCGGTTCAG
    GACATGAAGA CAGGGGAGGG TGGTCTCCTC CAAGTCACAC TGGAAGGCAT CAACCTCAAA
    TTTATGCACA GCCAGGAGCG GAAGGTTTTC ATAGAGCTGA ATCACATTAA AAAGTGCAAT
    ACAGTGAAAG GAGTCTTTGT CCTGGAGGAG TTTGTTCCTG AAACTAAAGA AGTGGTGATC
    CATAAGTACA AGACTCCTAT GGCACATCAG ATCTGTTACT CGGTGCTCTG CCTCTTCTCC
    TACATGGCGG CGGTCAAGGG GAAAGAGTCA GAGGGCAAAC CCAAGATGCT GTCCCCTCGT
    CCACTAGCGA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48929
    Clone ID Related Accession (Same CDS sequence) XM_009303280.2
    Accession Version XM_009303280.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4923bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product MAP kinase-activating death domain protein isoform X17
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303280.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGTG TCTGGTAAGG
    TTGACTCTGA TCACCCAAAA GCAGATCTTC GCCTCCGATT CTAAGGCTCT GGCTAGCATG
    AGTCTAAACA CACAGCCTAT TCTGAACCTG GAGAAGTTTC AGGATGGACA GGAGTTGTCT
    TTATTGCCCC CCGGTCGTGA CAAAGCTTCC CCGTCCTCCA CTGAGTTCAA CCCACTCATC
    TACGGCAATG ATGTGGACTC AGTTGATGTT GCCACAAGGG TGGCAATGGT GAGGTTCTTC
    AACTCTCCAA ACGTGCTGCA AGGGTTCCAG ATGCACACAC GCACTCTCCG TCTCTTCCCT
    CGTCCTGTGG TGGCCTTTCA GTCCACATCT TTCCTGGCTT CTAGGCCACG ACGAAATGGA
    TTCACAGAAA AGCTCTCCCA CACTCAGGCA GTGGAGTACT ATGGAGAATG GGCTCTCAAC
    CCCACCAACC TGGCTTTCCA AAGGATTCAT AACAATGTGT ATGACCCCTC TCTAATAGGC
    GATAAGCCTA AGTGGTATGC CCACCAGTTA CAGCCAGTAT TCTACCGCGT GTATGATGGA
    AACTCTCATT TAGCGGAGGC CTTGAGTGGA CCCCTGCAGG ATGAGACCAA TGATTCAGAC
    CCAACAGACG ACAGTGGCAG CGACAGTGAA GCATATGATG ACTCCAGCTC CTCCTACTCA
    TCTCTTGGAG ATTTTGTGAA TGAGATGATC AAAGGGGACA TACAGGGAGA CACACCAAAT
    GTTGATCCCC TGACCCATGC TGCACTGGGA GATGCAGATG AGGTTGAGAT CCATGACTTT
    CACGAGTATA AGGGGAACAG CGGTGAACCA GAGCCAGAAG GCCCCACAGA AGCAGCGGAC
    AGTCAGCCCT TGCGGTCCAG CTCTAGCACC ACAGCCAGTT CCAGCCCCAG TACAGTCATA
    CAGGGAGTCA ACCATGAGCA GAAGGAACCT GTTGAGGTGG AGCCCATAGC AAATGTGACT
    TACCCAAGCT CTGCCCCAGT ATTGGGTCCT CCTCCTTTTA CAAGACCCAC CCCTGATCCA
    ACCCTTGTTG ACCCTGCCAA TAAGAAGCGA GAATATGACA ACCCCTATTT TGAGCCCCAG
    TACGGTTTCC CTACAGAGGA AGATGCTGAG ACTGATGAGC AGGAGGAGAG TTACACACCA
    CGATTTAACC AGAATCTCAA TGGCAATAAG CCTTCTCGTC CACTGAGACC CAGCAGCCTG
    AAGCTTCCGG GTGAATCTGA CGGAGAGGGA GACTCCAGAA ATAGCTCTCC TAACTCAACC
    ATCTCCAACA ATAGCAGTGA TGGCTTTGGG GGACTCATGT CCTTTGCCAG TAACTTGTAT
    AAAAACCATG GCACCAGTTT CAGTCTGTCC AGCCTGGCTC TGCCTAATAA AGCGAGAGAG
    AAGAACACGC CGTTTCCCAG CCTCAAAGTA TTTGGGCTAA ATTCTCTAAT GGAGATTGTA
    ACAGAGATCG GCCCGGGGAG CGGAGAAGGT GCCCGAGCCC CCAGAGCACT GGTAGATCAG
    AAATCCTCTG TTATTAAGCA CAGCCCTACT GTGAAGAGAG AGTCTCCGTC TCCTCAAGGC
    AGAGCCAACA ATACCAGTGA GAACCAGCAG TTTCTAAAGG AGGTGGTCCA GAGCGTGCTG
    GAGGGGCAGG GTGTGGGCTG GCTTAACATG AAGAAGGTGC GTCGCCTGCT AGAGAATGAA
    CAGCTCAGAG TCTTTGTGCT CAGCAAACTC AACCGTGCGG TCCAGTCAGA GGAGGACGCC
    CAGCAGGAAG TTATAAGAGA TGTGGAGATA AATAGAAAGG TCTACAAAGG CATGCTGGAT
    ATTTTGAAGT GCACGGTGTC CAGTTTGGAG CATTCATACA CTAATGCTGG CTTAGGGGGC
    ATGGCCAGTG TCTTCAGTCT GCTAGAGATA GCACGCACCC ACTACCAAAC CAAAGATCCA
    GAGAAGCGGA AACGGAGCCC CACCGAAGGG CTTAGCAGTC CAGGCAGTAA GGAGAGCCCA
    TCGGGCCGAA TGGAGAGTGC CAGGGCGGCA GGGGTGCTTC TGGTACCCCG TATCCAATTG
    CCGCCACCCT CCTCTGGGAA ATCATCACGC CAGTTTGATA CCCGGAGTCT GAACGAGGAG
    AATTTTATTG CCTCCATTGG AGCGGATGGA GTAAAACAAC GTGTAGAAGG AGGAGACACT
    GAGGAGAAGA AATCACAGAT CAGTGCAGAC AGTGGCCTCA GTGTGACATC TGGCTCACAG
    AAGAGTGATA CGGATTCCCT GGCGAGCTCT GAACCTCCTG CTCTGACCAG AAGCACCAGC
    CAGGACTCAG AGGCCAGCAC AGTGGTAAGT AACAGCTCAG GTGAGACGCT CGGTGCAGAC
    AGTGACCTGA GCAGCACTGC TGGAGATGGC CTGACAGGAA GACATGGTCA GCATCTCAAC
    CTGTCCCGTG GCACACTGTC TGACAGTGAG ATTGAGACCA ACCCTGCAAC CAGCTCAGTG
    TTTGGCAAGA CACACAAGCT GAAGCCAGGA GTGAAGGAGC CTGTTGGTGT TAATAAAGCA
    GCACCTGCTC CACCCGTTGA GGATGTTAGC ATGAGGATCT ATCTGTGTGA GGGGCTTCTG
    GGTAAAGAGC GCTCAACTCT GTGGGATCAG ATGCAGTTCT GGGAGGACGC CTTCCTGGAT
    GCCGTAATGC TGGAACGGGA AGGAATGGGA ATGGATCAGG GACCACAAGA AATGATTGAC
    AGGTATCTTT CTCTGGGAGA TCATGACCGC AAGAGACTGG AGGATGATGA GGACAGACTG
    CTGGCCACGC TTCTGCACAA TATGATTGCT TACATGCTTA TGATGAAGGT CGGTAAAAAC
    GACATCAGGA AGAAAGTACG TCGTCTTATG GGCAAATCCC ACATTGGCCT CACTCACAGT
    CAGGAGATCA ATGAAGTCCT GGATCGCCTC GCACATTTAA GCGGTCGGGA GCTTTCTATC
    AGGCCGAGTG GCAGCAGGCA TATTAAAAAA CAGACATTTG TTGTGCATGC TGGGACAGAT
    ACAACTGGTG ACATATTCTT CATGGAGGTG TGTGATGATT GCATCGTCTT GCGCAGTAAT
    ATTGGCACAG TCTATGAGCG CTGGTGGTAT GAGAAGCTCA TAAATATGAC TTACTGTCCC
    AAAACTAAAG TGTTGTGTCT GTGGAGGAGA AACGGCCAGG AGACGCAGCT AAACAAGTTT
    TACACTAAGA AGTGTCGGGA ATTGTACTAC TGTGTTAAAG ACAGCATGGA GCGTGCTGCT
    GCTCGACAAC AAAGCATCAA ACCAGGTCCA GAGCTTGGAG GCGAGTTTCC GGTTCAGGAC
    ATGAAGACAG GGGAGGGTGG TCTCCTCCAA GTCACACTGG AAGGCATCAA CCTCAAATTT
    ATGCACAGCC AGGTTTTCAT AGAGCTGAAT CACATTAAAA AGTGCAATAC AGTGAAAGGA
    GTCTTTGTCC TGGAGGAGTT TGTTCCTGAA ACTAAAGAAG TGGTGATCCA TAAGTACAAG
    ACTCCTATGG CACATCAGAT CTGTTACTCG GTGCTCTGCC TCTTCTCCTA CATGGCGGCG
    GTCAAGGGGA AAGAGTCAGA GGGCAAACCC AAGATGCTGT CCCCTCGTCC ACTAGCGAGC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301555.1
    CDS435..5357
    Translation

    Target ORF information:

    RefSeq Version XM_009303280.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303280.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGTG TCTGGTAAGG
    TTGACTCTGA TCACCCAAAA GCAGATCTTC GCCTCCGATT CTAAGGCTCT GGCTAGCATG
    AGTCTAAACA CACAGCCTAT TCTGAACCTG GAGAAGTTTC AGGATGGACA GGAGTTGTCT
    TTATTGCCCC CCGGTCGTGA CAAAGCTTCC CCGTCCTCCA CTGAGTTCAA CCCACTCATC
    TACGGCAATG ATGTGGACTC AGTTGATGTT GCCACAAGGG TGGCAATGGT GAGGTTCTTC
    AACTCTCCAA ACGTGCTGCA AGGGTTCCAG ATGCACACAC GCACTCTCCG TCTCTTCCCT
    CGTCCTGTGG TGGCCTTTCA GTCCACATCT TTCCTGGCTT CTAGGCCACG ACGAAATGGA
    TTCACAGAAA AGCTCTCCCA CACTCAGGCA GTGGAGTACT ATGGAGAATG GGCTCTCAAC
    CCCACCAACC TGGCTTTCCA AAGGATTCAT AACAATGTGT ATGACCCCTC TCTAATAGGC
    GATAAGCCTA AGTGGTATGC CCACCAGTTA CAGCCAGTAT TCTACCGCGT GTATGATGGA
    AACTCTCATT TAGCGGAGGC CTTGAGTGGA CCCCTGCAGG ATGAGACCAA TGATTCAGAC
    CCAACAGACG ACAGTGGCAG CGACAGTGAA GCATATGATG ACTCCAGCTC CTCCTACTCA
    TCTCTTGGAG ATTTTGTGAA TGAGATGATC AAAGGGGACA TACAGGGAGA CACACCAAAT
    GTTGATCCCC TGACCCATGC TGCACTGGGA GATGCAGATG AGGTTGAGAT CCATGACTTT
    CACGAGTATA AGGGGAACAG CGGTGAACCA GAGCCAGAAG GCCCCACAGA AGCAGCGGAC
    AGTCAGCCCT TGCGGTCCAG CTCTAGCACC ACAGCCAGTT CCAGCCCCAG TACAGTCATA
    CAGGGAGTCA ACCATGAGCA GAAGGAACCT GTTGAGGTGG AGCCCATAGC AAATGTGACT
    TACCCAAGCT CTGCCCCAGT ATTGGGTCCT CCTCCTTTTA CAAGACCCAC CCCTGATCCA
    ACCCTTGTTG ACCCTGCCAA TAAGAAGCGA GAATATGACA ACCCCTATTT TGAGCCCCAG
    TACGGTTTCC CTACAGAGGA AGATGCTGAG ACTGATGAGC AGGAGGAGAG TTACACACCA
    CGATTTAACC AGAATCTCAA TGGCAATAAG CCTTCTCGTC CACTGAGACC CAGCAGCCTG
    AAGCTTCCGG GTGAATCTGA CGGAGAGGGA GACTCCAGAA ATAGCTCTCC TAACTCAACC
    ATCTCCAACA ATAGCAGTGA TGGCTTTGGG GGACTCATGT CCTTTGCCAG TAACTTGTAT
    AAAAACCATG GCACCAGTTT CAGTCTGTCC AGCCTGGCTC TGCCTAATAA AGCGAGAGAG
    AAGAACACGC CGTTTCCCAG CCTCAAAGTA TTTGGGCTAA ATTCTCTAAT GGAGATTGTA
    ACAGAGATCG GCCCGGGGAG CGGAGAAGGT GCCCGAGCCC CCAGAGCACT GGTAGATCAG
    AAATCCTCTG TTATTAAGCA CAGCCCTACT GTGAAGAGAG AGTCTCCGTC TCCTCAAGGC
    AGAGCCAACA ATACCAGTGA GAACCAGCAG TTTCTAAAGG AGGTGGTCCA GAGCGTGCTG
    GAGGGGCAGG GTGTGGGCTG GCTTAACATG AAGAAGGTGC GTCGCCTGCT AGAGAATGAA
    CAGCTCAGAG TCTTTGTGCT CAGCAAACTC AACCGTGCGG TCCAGTCAGA GGAGGACGCC
    CAGCAGGAAG TTATAAGAGA TGTGGAGATA AATAGAAAGG TCTACAAAGG CATGCTGGAT
    ATTTTGAAGT GCACGGTGTC CAGTTTGGAG CATTCATACA CTAATGCTGG CTTAGGGGGC
    ATGGCCAGTG TCTTCAGTCT GCTAGAGATA GCACGCACCC ACTACCAAAC CAAAGATCCA
    GAGAAGCGGA AACGGAGCCC CACCGAAGGG CTTAGCAGTC CAGGCAGTAA GGAGAGCCCA
    TCGGGCCGAA TGGAGAGTGC CAGGGCGGCA GGGGTGCTTC TGGTACCCCG TATCCAATTG
    CCGCCACCCT CCTCTGGGAA ATCATCACGC CAGTTTGATA CCCGGAGTCT GAACGAGGAG
    AATTTTATTG CCTCCATTGG AGCGGATGGA GTAAAACAAC GTGTAGAAGG AGGAGACACT
    GAGGAGAAGA AATCACAGAT CAGTGCAGAC AGTGGCCTCA GTGTGACATC TGGCTCACAG
    AAGAGTGATA CGGATTCCCT GGCGAGCTCT GAACCTCCTG CTCTGACCAG AAGCACCAGC
    CAGGACTCAG AGGCCAGCAC AGTGGTAAGT AACAGCTCAG GTGAGACGCT CGGTGCAGAC
    AGTGACCTGA GCAGCACTGC TGGAGATGGC CTGACAGGAA GACATGGTCA GCATCTCAAC
    CTGTCCCGTG GCACACTGTC TGACAGTGAG ATTGAGACCA ACCCTGCAAC CAGCTCAGTG
    TTTGGCAAGA CACACAAGCT GAAGCCAGGA GTGAAGGAGC CTGTTGGTGT TAATAAAGCA
    GCACCTGCTC CACCCGTTGA GGATGTTAGC ATGAGGATCT ATCTGTGTGA GGGGCTTCTG
    GGTAAAGAGC GCTCAACTCT GTGGGATCAG ATGCAGTTCT GGGAGGACGC CTTCCTGGAT
    GCCGTAATGC TGGAACGGGA AGGAATGGGA ATGGATCAGG GACCACAAGA AATGATTGAC
    AGGTATCTTT CTCTGGGAGA TCATGACCGC AAGAGACTGG AGGATGATGA GGACAGACTG
    CTGGCCACGC TTCTGCACAA TATGATTGCT TACATGCTTA TGATGAAGGT CGGTAAAAAC
    GACATCAGGA AGAAAGTACG TCGTCTTATG GGCAAATCCC ACATTGGCCT CACTCACAGT
    CAGGAGATCA ATGAAGTCCT GGATCGCCTC GCACATTTAA GCGGTCGGGA GCTTTCTATC
    AGGCCGAGTG GCAGCAGGCA TATTAAAAAA CAGACATTTG TTGTGCATGC TGGGACAGAT
    ACAACTGGTG ACATATTCTT CATGGAGGTG TGTGATGATT GCATCGTCTT GCGCAGTAAT
    ATTGGCACAG TCTATGAGCG CTGGTGGTAT GAGAAGCTCA TAAATATGAC TTACTGTCCC
    AAAACTAAAG TGTTGTGTCT GTGGAGGAGA AACGGCCAGG AGACGCAGCT AAACAAGTTT
    TACACTAAGA AGTGTCGGGA ATTGTACTAC TGTGTTAAAG ACAGCATGGA GCGTGCTGCT
    GCTCGACAAC AAAGCATCAA ACCAGGTCCA GAGCTTGGAG GCGAGTTTCC GGTTCAGGAC
    ATGAAGACAG GGGAGGGTGG TCTCCTCCAA GTCACACTGG AAGGCATCAA CCTCAAATTT
    ATGCACAGCC AGGTTTTCAT AGAGCTGAAT CACATTAAAA AGTGCAATAC AGTGAAAGGA
    GTCTTTGTCC TGGAGGAGTT TGTTCCTGAA ACTAAAGAAG TGGTGATCCA TAAGTACAAG
    ACTCCTATGG CACATCAGAT CTGTTACTCG GTGCTCTGCC TCTTCTCCTA CATGGCGGCG
    GTCAAGGGGA AAGAGTCAGA GGGCAAACCC AAGATGCTGT CCCCTCGTCC ACTAGCGAGC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48930
    Clone ID Related Accession (Same CDS sequence) XM_017357063.1
    Accession Version XM_017357063.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4890bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product MAP kinase-activating death domain protein isoform X18
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGTG TCTGGTAAGG
    TTGACTCTGA TCACCCAAAA GCAGATCTTC GCCTCCGATT CTAAGGCTCT GGCTAGCATG
    AGTCTAAACA CACAGCCTAT TCTGAACCTG GAGAAGTTTC AGGATGGACA GGAGTTGTCT
    TTATTGCCCC CCGGTCGTGA CAAAGCTTCC CCGTCCTCCA CTGAGTTCAA CCCACTCATC
    TACGGCAATG ATGTGGACTC AGTTGATGTT GCCACAAGGG TGGCAATGGT GAGGTTCTTC
    AACTCTCCAA ACGTGCTGCA AGGGTTCCAG ATGCACACAC GCACTCTCCG TCTCTTCCCT
    CGTCCTGTGG TGGCCTTTCA GTCCACATCT TTCCTGGCTT CTAGGCCACG ACGAAATGGA
    TTCACAGAAA AGCTCTCCCA CACTCAGGCA GTGGAGTACT ATGGAGAATG GGCTCTCAAC
    CCCACCAACC TGGCTTTCCA AAGGATTCAT AACAATGTGT ATGACCCCTC TCTAATAGGC
    GATAAGCCTA AGTGGTATGC CCACCAGTTA CAGCCAGTAT TCTACCGCGT GTATGATGGA
    AACTCTCATT TAGCGGAGGC CTTGAGTGGA CCCCTGCAGG ATGAGACCAA TGATTCAGAC
    CCAACAGACG ACAGTGGCAG CGACAGTGAA GCATATGATG ACTCCAGCTC CTCCTACTCA
    TCTCTTGGAG ATTTTGTGAA TGAGATGATC AAAGGGGACA TACAGGGAGA CACACCAAAT
    GTTGATCCCC TGACCCATGC TGCACTGGGA GATGCAGATG AGGTTGAGAT CCATGACTTT
    CACGAGTATA AGGGGAACAG CGGTGAACCA GAGCCAGAAG GCCCCACAGA AGCAGCGGAC
    AGTCAGCCCT TGCGGTCCAG CTCTAGCACC ACAGCCAGTT CCAGCCCCAG TACAGTCATA
    CAGGGAGTCA ACCATGAGCA GAAGGAACCT GTTGAGGTGG AGCCCATAGC AAATGTGACT
    TACCCAAGCT CTGCCCCAGT ATTGGGTCCT CCTCCTTTTA CAAGACCCAC CCCTGATCCA
    ACCCTTGTTG ACCCTGCCAA TAAGAAGCGA GAATATGACA ACCCCTATTT TGAGCCCCAG
    TACGGTTTCC CTACAGAGGA AGATGCTGAG ACTGATGAGC AGGAGGAGAG TTACACACCA
    CGATTTAACC AGAATCTCAA TGGCAATAAG CCTTCTCGTC CACTGAGACC CAGCAGCCTG
    AAGCTTCCGG GTGAATCTGA CGGAGAGGGA GACTCCAGAA ATAGCTCTCC TAACTCAACC
    ATCTCCAACA ATAGCAGTGA TGGCTTTGGG GGACTCATGT CCTTTGCCAG TAACTTGTAT
    AAAAACCATG GCACCAGTTT CAGTCTGTCC AGCCTGGCTC TGCCTAATAA AGCGAGAGAG
    AAGAACACGC CGTTTCCCAG CCTCAAAGAC GATGCTGATG ACAGTCCTGA AAGTCCTGTA
    TTTGGGCTAA ATTCTCTAAT GGAGATTGTA ACAGAGATCG GCCCGGGGAG CGGAGAAGGT
    GCCCGAGCCC CCAGAGCACT GGTAGATCAG AAATCCTCTG TTATTAAGCA CAGCCCTACT
    GTGAAGAGAG AGTCTCCGTC TCCTCAAGGC AGAGCCAACA ATACCAGTGA GAACCAGCAG
    TTTCTAAAGG AGGTGGTCCA GAGCGTGCTG GAGGGGCAGG GTGTGGGCTG GCTTAACATG
    AAGAAGGTGC GTCGCCTGCT AGAGAATGAA CAGCTCAGAG TCTTTGTGCT CAGCAAACTC
    AACCGTGCGG TCCAGTCAGA GGAGGACGCC CAGCAGGAAG TTATAAGAGA TGTGGAGATA
    AATAGAAAGG TCTACAAAGG CATGCTGGAT ATTTTGAAGT GCACGGTGTC CAGTTTGGAG
    CATTCATACA CTAATGCTGG CTTAGGGGGC ATGGCCAGTG TCTTCAGTCT GCTAGAGATA
    GCACGCACCC ACTACCAAAC CAAAGATCCA GAGAAGCGGA AACGGAGCCC CACCGAAGGG
    CTTAGCAGTC CAGGCAGTAA GGAGAGCCCA TCGGGCCGAA TGGAGAGTGC CAGGGCGGCA
    GGGGTGCTTC TGGTACCCCG TATCCAATTG CCGCCACCCT CCTCTGGGAA ATCATCACGC
    CAGTTTGATA CCCGGAGTCT GAACGAGGAG AATTTTATTG CCTCCATTGA ATTGTGGAGC
    AAGCACCAGG ATAACAGAAA GCAAAAAGCT TTGGAAAAAG AACAGAGTGG TTTAAATAAA
    CGGAAATCAG GAGCGGATGG AGTAAAACAA CGTGTAGAAG GAGGAGACAC TGAGGAGAAG
    AAATCACAGA TCAGTGCAGA CAGTGGCCTC AGTGTGACAT CTGGCTCACA GAAGAGTGAT
    ACGGATTCCC TGGCGAGCTC TGAACCTCCT GCTCTGACCA GAAGCACCAG CCAGGACTCA
    GAGGCCAGCA CAGTGGTAAG TAACAGCTCA GGTGAGACGC TCGGTGCAGA CAGTGACCTG
    AGCAGCACTG CTGGAGATGG CCTGACAGGA AGACATGGTC AGCATCTCAA CCTGTCCCGT
    GGCACACTGT CTGACAGTGA GATTGAGACC AACCCTGCAA CCAGCTCAGT GTTTGGCAAG
    ACACACAAGC TGAAGCCAGG AGTGAAGGAG CCTGTTGGTG TTAATAAAGC AGCACCTGCT
    CCACCCGTTG AGGATGTTAG CATGAGGATC TATCTGTGTG AGGGGCTTCT GGGCCGGGAT
    AAAAGCTCAG TTTGGGACCA GTTGGAGGAT GCTGCAATGG AAACCTTTTC TCTGAGTAAA
    GAGCGCTCAA CTCTGTGGGA TCAGATGCAG TTCTGGGAGG ACGCCTTCCT GGATGCCGTA
    ATGCTGGAAC GGGAAGGAAT GGGAATGGAT CAGGGACCAC AAGAAATGAT TGACAGGTAT
    CTTTCTCTGG GAGATCATGA CCGCAAGAGA CTGGAGGATG ATGAGGACAG ACTGCTGGCC
    ACGCTTCTGC ACAATATGAT TGCTTACATG CTTATGATGA AGGTCGGTAA AAACGACATC
    AGGAAGAAAG TACGTCGTCT TATGGGCAAA TCCCACATTG GCCTCACTCA CAGTCAGGAG
    ATCAATGAAG TCCTGGATCG CCTCGCACAT TTAAGCGGTC GGGAGCTTTC TATCAGGCCG
    AGTGGCAGCA GGCATATTAA AAAACAGACA TTTGTTGTGC ATGCTGGGAC AGATACAACT
    GGTGACATAT TCTTCATGGA GGTGTGTGAT GATTGCATCG TCTTGCGCAG TAATATTGGC
    ACAGTCTATG AGCGCTGGTG GTATGAGAAG CTCATAAATA TGACTTACTG TCCCAAAACT
    AAAGTGTTGT GTCTGTGGAG GAGAAACGGC CAGGAGACGC AGCTAAACAA GTTTTACACT
    AAGAAGTGTC GGGAATTGTA CTACTGTGTT AAAGACAGCA TGGAGCGTGC TGCTGCTCGA
    CAACAAAGCA TCAAACCAGG TCCAGAGCTT GGAGGCGAGT TTCCGGTTCA GGACATGAAG
    ACAGGGGAGG GTGGTCTCCT CCAAGTCACA CTGGAAGGCA TCAACCTCAA ATTTATGCAC
    AGCCAGTTCC TGAAACTAAA GAAGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017212552.1
    CDS435..5324
    Translation

    Target ORF information:

    RefSeq Version XM_017357063.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017357063.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGTG TCTGGTAAGG
    TTGACTCTGA TCACCCAAAA GCAGATCTTC GCCTCCGATT CTAAGGCTCT GGCTAGCATG
    AGTCTAAACA CACAGCCTAT TCTGAACCTG GAGAAGTTTC AGGATGGACA GGAGTTGTCT
    TTATTGCCCC CCGGTCGTGA CAAAGCTTCC CCGTCCTCCA CTGAGTTCAA CCCACTCATC
    TACGGCAATG ATGTGGACTC AGTTGATGTT GCCACAAGGG TGGCAATGGT GAGGTTCTTC
    AACTCTCCAA ACGTGCTGCA AGGGTTCCAG ATGCACACAC GCACTCTCCG TCTCTTCCCT
    CGTCCTGTGG TGGCCTTTCA GTCCACATCT TTCCTGGCTT CTAGGCCACG ACGAAATGGA
    TTCACAGAAA AGCTCTCCCA CACTCAGGCA GTGGAGTACT ATGGAGAATG GGCTCTCAAC
    CCCACCAACC TGGCTTTCCA AAGGATTCAT AACAATGTGT ATGACCCCTC TCTAATAGGC
    GATAAGCCTA AGTGGTATGC CCACCAGTTA CAGCCAGTAT TCTACCGCGT GTATGATGGA
    AACTCTCATT TAGCGGAGGC CTTGAGTGGA CCCCTGCAGG ATGAGACCAA TGATTCAGAC
    CCAACAGACG ACAGTGGCAG CGACAGTGAA GCATATGATG ACTCCAGCTC CTCCTACTCA
    TCTCTTGGAG ATTTTGTGAA TGAGATGATC AAAGGGGACA TACAGGGAGA CACACCAAAT
    GTTGATCCCC TGACCCATGC TGCACTGGGA GATGCAGATG AGGTTGAGAT CCATGACTTT
    CACGAGTATA AGGGGAACAG CGGTGAACCA GAGCCAGAAG GCCCCACAGA AGCAGCGGAC
    AGTCAGCCCT TGCGGTCCAG CTCTAGCACC ACAGCCAGTT CCAGCCCCAG TACAGTCATA
    CAGGGAGTCA ACCATGAGCA GAAGGAACCT GTTGAGGTGG AGCCCATAGC AAATGTGACT
    TACCCAAGCT CTGCCCCAGT ATTGGGTCCT CCTCCTTTTA CAAGACCCAC CCCTGATCCA
    ACCCTTGTTG ACCCTGCCAA TAAGAAGCGA GAATATGACA ACCCCTATTT TGAGCCCCAG
    TACGGTTTCC CTACAGAGGA AGATGCTGAG ACTGATGAGC AGGAGGAGAG TTACACACCA
    CGATTTAACC AGAATCTCAA TGGCAATAAG CCTTCTCGTC CACTGAGACC CAGCAGCCTG
    AAGCTTCCGG GTGAATCTGA CGGAGAGGGA GACTCCAGAA ATAGCTCTCC TAACTCAACC
    ATCTCCAACA ATAGCAGTGA TGGCTTTGGG GGACTCATGT CCTTTGCCAG TAACTTGTAT
    AAAAACCATG GCACCAGTTT CAGTCTGTCC AGCCTGGCTC TGCCTAATAA AGCGAGAGAG
    AAGAACACGC CGTTTCCCAG CCTCAAAGAC GATGCTGATG ACAGTCCTGA AAGTCCTGTA
    TTTGGGCTAA ATTCTCTAAT GGAGATTGTA ACAGAGATCG GCCCGGGGAG CGGAGAAGGT
    GCCCGAGCCC CCAGAGCACT GGTAGATCAG AAATCCTCTG TTATTAAGCA CAGCCCTACT
    GTGAAGAGAG AGTCTCCGTC TCCTCAAGGC AGAGCCAACA ATACCAGTGA GAACCAGCAG
    TTTCTAAAGG AGGTGGTCCA GAGCGTGCTG GAGGGGCAGG GTGTGGGCTG GCTTAACATG
    AAGAAGGTGC GTCGCCTGCT AGAGAATGAA CAGCTCAGAG TCTTTGTGCT CAGCAAACTC
    AACCGTGCGG TCCAGTCAGA GGAGGACGCC CAGCAGGAAG TTATAAGAGA TGTGGAGATA
    AATAGAAAGG TCTACAAAGG CATGCTGGAT ATTTTGAAGT GCACGGTGTC CAGTTTGGAG
    CATTCATACA CTAATGCTGG CTTAGGGGGC ATGGCCAGTG TCTTCAGTCT GCTAGAGATA
    GCACGCACCC ACTACCAAAC CAAAGATCCA GAGAAGCGGA AACGGAGCCC CACCGAAGGG
    CTTAGCAGTC CAGGCAGTAA GGAGAGCCCA TCGGGCCGAA TGGAGAGTGC CAGGGCGGCA
    GGGGTGCTTC TGGTACCCCG TATCCAATTG CCGCCACCCT CCTCTGGGAA ATCATCACGC
    CAGTTTGATA CCCGGAGTCT GAACGAGGAG AATTTTATTG CCTCCATTGA ATTGTGGAGC
    AAGCACCAGG ATAACAGAAA GCAAAAAGCT TTGGAAAAAG AACAGAGTGG TTTAAATAAA
    CGGAAATCAG GAGCGGATGG AGTAAAACAA CGTGTAGAAG GAGGAGACAC TGAGGAGAAG
    AAATCACAGA TCAGTGCAGA CAGTGGCCTC AGTGTGACAT CTGGCTCACA GAAGAGTGAT
    ACGGATTCCC TGGCGAGCTC TGAACCTCCT GCTCTGACCA GAAGCACCAG CCAGGACTCA
    GAGGCCAGCA CAGTGGTAAG TAACAGCTCA GGTGAGACGC TCGGTGCAGA CAGTGACCTG
    AGCAGCACTG CTGGAGATGG CCTGACAGGA AGACATGGTC AGCATCTCAA CCTGTCCCGT
    GGCACACTGT CTGACAGTGA GATTGAGACC AACCCTGCAA CCAGCTCAGT GTTTGGCAAG
    ACACACAAGC TGAAGCCAGG AGTGAAGGAG CCTGTTGGTG TTAATAAAGC AGCACCTGCT
    CCACCCGTTG AGGATGTTAG CATGAGGATC TATCTGTGTG AGGGGCTTCT GGGCCGGGAT
    AAAAGCTCAG TTTGGGACCA GTTGGAGGAT GCTGCAATGG AAACCTTTTC TCTGAGTAAA
    GAGCGCTCAA CTCTGTGGGA TCAGATGCAG TTCTGGGAGG ACGCCTTCCT GGATGCCGTA
    ATGCTGGAAC GGGAAGGAAT GGGAATGGAT CAGGGACCAC AAGAAATGAT TGACAGGTAT
    CTTTCTCTGG GAGATCATGA CCGCAAGAGA CTGGAGGATG ATGAGGACAG ACTGCTGGCC
    ACGCTTCTGC ACAATATGAT TGCTTACATG CTTATGATGA AGGTCGGTAA AAACGACATC
    AGGAAGAAAG TACGTCGTCT TATGGGCAAA TCCCACATTG GCCTCACTCA CAGTCAGGAG
    ATCAATGAAG TCCTGGATCG CCTCGCACAT TTAAGCGGTC GGGAGCTTTC TATCAGGCCG
    AGTGGCAGCA GGCATATTAA AAAACAGACA TTTGTTGTGC ATGCTGGGAC AGATACAACT
    GGTGACATAT TCTTCATGGA GGTGTGTGAT GATTGCATCG TCTTGCGCAG TAATATTGGC
    ACAGTCTATG AGCGCTGGTG GTATGAGAAG CTCATAAATA TGACTTACTG TCCCAAAACT
    AAAGTGTTGT GTCTGTGGAG GAGAAACGGC CAGGAGACGC AGCTAAACAA GTTTTACACT
    AAGAAGTGTC GGGAATTGTA CTACTGTGTT AAAGACAGCA TGGAGCGTGC TGCTGCTCGA
    CAACAAAGCA TCAAACCAGG TCCAGAGCTT GGAGGCGAGT TTCCGGTTCA GGACATGAAG
    ACAGGGGAGG GTGGTCTCCT CCAAGTCACA CTGGAAGGCA TCAACCTCAA ATTTATGCAC
    AGCCAGTTCC TGAAACTAAA GAAGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48931
    Clone ID Related Accession (Same CDS sequence) XM_009303282.2
    Accession Version XM_009303282.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4887bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product MAP kinase-activating death domain protein isoform X19
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303282.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGTG TCTGGTAAGG
    TTGACTCTGA TCACCCAAAA GCAGATCTTC GCCTCCGATT CTAAGGCTCT GGCTAGCATG
    AGTCTAAACA CACAGCCTAT TCTGAACCTG GAGAAGTTTC AGGATGGACA GGAGTTGTCT
    TTATTGCCCC CCGGTCGTGA CAAAGCTTCC CCGTCCTCCA CTGAGTTCAA CCCACTCATC
    TACGGCAATG ATGTGGACTC AGTTGATGTT GCCACAAGGG TGGCAATGGT GAGGTTCTTC
    AACTCTCCAA ACGTGCTGCA AGGGTTCCAG ATGCACACAC GCACTCTCCG TCTCTTCCCT
    CGTCCTGTGG TGGCCTTTCA GTCCACATCT TTCCTGGCTT CTAGGCCACG ACGAAATGGA
    TTCACAGAAA AGCTCTCCCA CACTCAGGCA GTGGAGTACT ATGGAGAATG GGCTCTCAAC
    CCCACCAACC TGGCTTTCCA AAGGATTCAT AACAATGTGT ATGACCCCTC TCTAATAGGC
    GATAAGCCTA AGTGGTATGC CCACCAGTTA CAGCCAGTAT TCTACCGCGT GTATGATGGA
    AACTCTCATT TAGCGGAGGC CTTGAGTGGA CCCCTGCAGG ATGAGACCAA TGATTCAGAC
    CCAACAGACG ACAGTGGCAG CGACAGTGAA GCATATGATG ACTCCAGCTC CTCCTACTCA
    TCTCTTGGAG ATTTTGTGAA TGAGATGATC AAAGGGGACA TACAGGGAGA CACACCAAAT
    GTTGATCCCC TGACCCATGC TGCACTGGGA GATGCAGATG AGGTTGAGAT CCATGACTTT
    CACGAGTATA AGGGGAACAG CGGTGAACCA GAGCCAGAAG GCCCCACAGA AGCAGCGGAC
    AGTCAGCCCT TGCGGTCCAG CTCTAGCACC ACAGCCAGTT CCAGCCCCAG TACAGTCATA
    CAGGGAGTCA ACCATGAGCA GAAGGAACCT GTTGAGGTGG AGCCCATAGC AAATGTGACT
    TACCCAAGCT CTGCCCCAGT ATTGGGTCCT CCTCCTTTTA CAAGACCCAC CCCTGATCCA
    ACCCTTGTTG ACCCTGCCAA TAAGAAGCGA GAATATGACA ACCCCTATTT TGAGCCCCAG
    TACGGTTTCC CTACAGAGGA AGATGCTGAG ACTGATGAGC AGGAGGAGAG TTACACACCA
    CGATTTAACC AGAATCTCAA TGGCAATAAG CCTTCTCGTC CACTGAGACC CAGCAGCCTG
    AAGCTTCCGG GTGAATCTGA CGGAGAGGGA GACTCCAGAA ATAGCTCTCC TAACTCAACC
    ATCTCCAACA ATAGCAGTGA TGGCTTTGGG GGACTCATGT CCTTTGCCAG TAACTTGTAT
    AAAAACCATG GCACCAGTTT CAGTCTGTCC AGCCTGGCTC TGCCTAATAA AGCGAGAGAG
    AAGAACACGC CGTTTCCCAG CCTCAAAGAC GATGCTGATG ACAGTCCTGA AAGTCCTGTA
    TTTGGGCTAA ATTCTCTAAT GGAGATTGTA ACAGAGATCG GCCCGGGGAG CGGAGAAGGT
    GCCCGAGCCC CCAGAGCACT GGTAGATCAG AAATCCTCTG TTATTAAGCA CAGCCCTACT
    GTGAAGAGAG AGTCTCCGTC TCCTCAAGGC AGAGCCAACA ATACCAGTGA GAACCAGCAG
    TTTCTAAAGG AGGTGGTCCA GAGCGTGCTG GAGGGGCAGG GTGTGGGCTG GCTTAACATG
    AAGAAGGTGC GTCGCCTGCT AGAGAATGAA CAGCTCAGAG TCTTTGTGCT CAGCAAACTC
    AACCGTGCGG TCCAGTCAGA GGAGGACGCC CAGCAGGAAG TTATAAGAGA TGTGGAGATA
    AATAGAAAGG TCTACAAAGG CATGCTGGAT ATTTTGAAGT GCACGGTGTC CAGTTTGGAG
    CATTCATACA CTAATGCTGG CTTAGGGGGC ATGGCCAGTG TCTTCAGTCT GCTAGAGATA
    GCACGCACCC ACTACCAAAC CAAAGATCCA GAGAAGCGGA AACGGAGCCC CACCGAAGGG
    CTTAGCAGTC CAGGCAGTAA GGAGAGCCCA TCGGGCCGAA TGGAGAGTGC CAGGGCGGCA
    GGGGTGCTTC TGGTACCCCG TATCCAATTG CCGCCACCCT CCTCTGGGAA ATCATCACGC
    CAGTTTGATA CCCGGAGTCT GAACGAGGAG AATTTTATTG CCTCCATTGA ATTGTGGAGC
    AAGCACCAGG ATAACAGAAA GCAAAAAGCT TTGGAAAAAG AACAGAGTGG TTTAAATAAA
    CGGAAATCAG GAGCGGATGG AGTAAAACAA CGTGTAGAAG GAGGAGACAC TGAGGAGAAG
    AAATCACAGA TCAGTGCAGA CAGTGGCCTC AGTGTGACAT CTGGCTCACA GAAGAGTGAT
    ACGGATTCCC TGGCGAGCTC TGAACCTCCT GCTCTGACCA GAAGCACCAG CCAGGACTCA
    GAGGCCAGCA CAGTAAGTAA CAGCTCAGGT GAGACGCTCG GTGCAGACAG TGACCTGAGC
    AGCACTGCTG GAGATGGCCT GACAGGAAGA CATGGTCAGC ATCTCAACCT GTCCCGTGGC
    ACACTGTCTG ACAGTGAGAT TGAGACCAAC CCTGCAACCA GCTCAGTGTT TGGCAAGACA
    CACAAGCTGA AGCCAGGAGT GAAGGAGCCT GTTGGTGTTA ATAAAGCAGC ACCTGCTCCA
    CCCGTTGAGG ATGTTAGCAT GAGGATCTAT CTGTGTGAGG GGCTTCTGGG CCGGGATAAA
    AGCTCAGTTT GGGACCAGTT GGAGGATGCT GCAATGGAAA CCTTTTCTCT GAGTAAAGAG
    CGCTCAACTC TGTGGGATCA GATGCAGTTC TGGGAGGACG CCTTCCTGGA TGCCGTAATG
    CTGGAACGGG AAGGAATGGG AATGGATCAG GGACCACAAG AAATGATTGA CAGGTATCTT
    TCTCTGGGAG ATCATGACCG CAAGAGACTG GAGGATGATG AGGACAGACT GCTGGCCACG
    CTTCTGCACA ATATGATTGC TTACATGCTT ATGATGAAGG TCGGTAAAAA CGACATCAGG
    AAGAAAGTAC GTCGTCTTAT GGGCAAATCC CACATTGGCC TCACTCACAG TCAGGAGATC
    AATGAAGTCC TGGATCGCCT CGCACATTTA AGCGGTCGGG AGCTTTCTAT CAGGCCGAGT
    GGCAGCAGGC ATATTAAAAA ACAGACATTT GTTGTGCATG CTGGGACAGA TACAACTGGT
    GACATATTCT TCATGGAGGT GTGTGATGAT TGCATCGTCT TGCGCAGTAA TATTGGCACA
    GTCTATGAGC GCTGGTGGTA TGAGAAGCTC ATAAATATGA CTTACTGTCC CAAAACTAAA
    GTGTTGTGTC TGTGGAGGAG AAACGGCCAG GAGACGCAGC TAAACAAGTT TTACACTAAG
    AAGTGTCGGG AATTGTACTA CTGTGTTAAA GACAGCATGG AGCGTGCTGC TGCTCGACAA
    CAAAGCATCA AACCAGGTCC AGAGCTTGGA GGCGAGTTTC CGGTTCAGGA CATGAAGACA
    GGGGAGGGTG GTCTCCTCCA AGTCACACTG GAAGGCATCA ACCTCAAATT TATGCACAGC
    CAGTTCCTGA AACTAAAGAA GTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301557.1
    CDS435..5321
    Translation

    Target ORF information:

    RefSeq Version XM_009303282.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303282.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGTG TCTGGTAAGG
    TTGACTCTGA TCACCCAAAA GCAGATCTTC GCCTCCGATT CTAAGGCTCT GGCTAGCATG
    AGTCTAAACA CACAGCCTAT TCTGAACCTG GAGAAGTTTC AGGATGGACA GGAGTTGTCT
    TTATTGCCCC CCGGTCGTGA CAAAGCTTCC CCGTCCTCCA CTGAGTTCAA CCCACTCATC
    TACGGCAATG ATGTGGACTC AGTTGATGTT GCCACAAGGG TGGCAATGGT GAGGTTCTTC
    AACTCTCCAA ACGTGCTGCA AGGGTTCCAG ATGCACACAC GCACTCTCCG TCTCTTCCCT
    CGTCCTGTGG TGGCCTTTCA GTCCACATCT TTCCTGGCTT CTAGGCCACG ACGAAATGGA
    TTCACAGAAA AGCTCTCCCA CACTCAGGCA GTGGAGTACT ATGGAGAATG GGCTCTCAAC
    CCCACCAACC TGGCTTTCCA AAGGATTCAT AACAATGTGT ATGACCCCTC TCTAATAGGC
    GATAAGCCTA AGTGGTATGC CCACCAGTTA CAGCCAGTAT TCTACCGCGT GTATGATGGA
    AACTCTCATT TAGCGGAGGC CTTGAGTGGA CCCCTGCAGG ATGAGACCAA TGATTCAGAC
    CCAACAGACG ACAGTGGCAG CGACAGTGAA GCATATGATG ACTCCAGCTC CTCCTACTCA
    TCTCTTGGAG ATTTTGTGAA TGAGATGATC AAAGGGGACA TACAGGGAGA CACACCAAAT
    GTTGATCCCC TGACCCATGC TGCACTGGGA GATGCAGATG AGGTTGAGAT CCATGACTTT
    CACGAGTATA AGGGGAACAG CGGTGAACCA GAGCCAGAAG GCCCCACAGA AGCAGCGGAC
    AGTCAGCCCT TGCGGTCCAG CTCTAGCACC ACAGCCAGTT CCAGCCCCAG TACAGTCATA
    CAGGGAGTCA ACCATGAGCA GAAGGAACCT GTTGAGGTGG AGCCCATAGC AAATGTGACT
    TACCCAAGCT CTGCCCCAGT ATTGGGTCCT CCTCCTTTTA CAAGACCCAC CCCTGATCCA
    ACCCTTGTTG ACCCTGCCAA TAAGAAGCGA GAATATGACA ACCCCTATTT TGAGCCCCAG
    TACGGTTTCC CTACAGAGGA AGATGCTGAG ACTGATGAGC AGGAGGAGAG TTACACACCA
    CGATTTAACC AGAATCTCAA TGGCAATAAG CCTTCTCGTC CACTGAGACC CAGCAGCCTG
    AAGCTTCCGG GTGAATCTGA CGGAGAGGGA GACTCCAGAA ATAGCTCTCC TAACTCAACC
    ATCTCCAACA ATAGCAGTGA TGGCTTTGGG GGACTCATGT CCTTTGCCAG TAACTTGTAT
    AAAAACCATG GCACCAGTTT CAGTCTGTCC AGCCTGGCTC TGCCTAATAA AGCGAGAGAG
    AAGAACACGC CGTTTCCCAG CCTCAAAGAC GATGCTGATG ACAGTCCTGA AAGTCCTGTA
    TTTGGGCTAA ATTCTCTAAT GGAGATTGTA ACAGAGATCG GCCCGGGGAG CGGAGAAGGT
    GCCCGAGCCC CCAGAGCACT GGTAGATCAG AAATCCTCTG TTATTAAGCA CAGCCCTACT
    GTGAAGAGAG AGTCTCCGTC TCCTCAAGGC AGAGCCAACA ATACCAGTGA GAACCAGCAG
    TTTCTAAAGG AGGTGGTCCA GAGCGTGCTG GAGGGGCAGG GTGTGGGCTG GCTTAACATG
    AAGAAGGTGC GTCGCCTGCT AGAGAATGAA CAGCTCAGAG TCTTTGTGCT CAGCAAACTC
    AACCGTGCGG TCCAGTCAGA GGAGGACGCC CAGCAGGAAG TTATAAGAGA TGTGGAGATA
    AATAGAAAGG TCTACAAAGG CATGCTGGAT ATTTTGAAGT GCACGGTGTC CAGTTTGGAG
    CATTCATACA CTAATGCTGG CTTAGGGGGC ATGGCCAGTG TCTTCAGTCT GCTAGAGATA
    GCACGCACCC ACTACCAAAC CAAAGATCCA GAGAAGCGGA AACGGAGCCC CACCGAAGGG
    CTTAGCAGTC CAGGCAGTAA GGAGAGCCCA TCGGGCCGAA TGGAGAGTGC CAGGGCGGCA
    GGGGTGCTTC TGGTACCCCG TATCCAATTG CCGCCACCCT CCTCTGGGAA ATCATCACGC
    CAGTTTGATA CCCGGAGTCT GAACGAGGAG AATTTTATTG CCTCCATTGA ATTGTGGAGC
    AAGCACCAGG ATAACAGAAA GCAAAAAGCT TTGGAAAAAG AACAGAGTGG TTTAAATAAA
    CGGAAATCAG GAGCGGATGG AGTAAAACAA CGTGTAGAAG GAGGAGACAC TGAGGAGAAG
    AAATCACAGA TCAGTGCAGA CAGTGGCCTC AGTGTGACAT CTGGCTCACA GAAGAGTGAT
    ACGGATTCCC TGGCGAGCTC TGAACCTCCT GCTCTGACCA GAAGCACCAG CCAGGACTCA
    GAGGCCAGCA CAGTAAGTAA CAGCTCAGGT GAGACGCTCG GTGCAGACAG TGACCTGAGC
    AGCACTGCTG GAGATGGCCT GACAGGAAGA CATGGTCAGC ATCTCAACCT GTCCCGTGGC
    ACACTGTCTG ACAGTGAGAT TGAGACCAAC CCTGCAACCA GCTCAGTGTT TGGCAAGACA
    CACAAGCTGA AGCCAGGAGT GAAGGAGCCT GTTGGTGTTA ATAAAGCAGC ACCTGCTCCA
    CCCGTTGAGG ATGTTAGCAT GAGGATCTAT CTGTGTGAGG GGCTTCTGGG CCGGGATAAA
    AGCTCAGTTT GGGACCAGTT GGAGGATGCT GCAATGGAAA CCTTTTCTCT GAGTAAAGAG
    CGCTCAACTC TGTGGGATCA GATGCAGTTC TGGGAGGACG CCTTCCTGGA TGCCGTAATG
    CTGGAACGGG AAGGAATGGG AATGGATCAG GGACCACAAG AAATGATTGA CAGGTATCTT
    TCTCTGGGAG ATCATGACCG CAAGAGACTG GAGGATGATG AGGACAGACT GCTGGCCACG
    CTTCTGCACA ATATGATTGC TTACATGCTT ATGATGAAGG TCGGTAAAAA CGACATCAGG
    AAGAAAGTAC GTCGTCTTAT GGGCAAATCC CACATTGGCC TCACTCACAG TCAGGAGATC
    AATGAAGTCC TGGATCGCCT CGCACATTTA AGCGGTCGGG AGCTTTCTAT CAGGCCGAGT
    GGCAGCAGGC ATATTAAAAA ACAGACATTT GTTGTGCATG CTGGGACAGA TACAACTGGT
    GACATATTCT TCATGGAGGT GTGTGATGAT TGCATCGTCT TGCGCAGTAA TATTGGCACA
    GTCTATGAGC GCTGGTGGTA TGAGAAGCTC ATAAATATGA CTTACTGTCC CAAAACTAAA
    GTGTTGTGTC TGTGGAGGAG AAACGGCCAG GAGACGCAGC TAAACAAGTT TTACACTAAG
    AAGTGTCGGG AATTGTACTA CTGTGTTAAA GACAGCATGG AGCGTGCTGC TGCTCGACAA
    CAAAGCATCA AACCAGGTCC AGAGCTTGGA GGCGAGTTTC CGGTTCAGGA CATGAAGACA
    GGGGAGGGTG GTCTCCTCCA AGTCACACTG GAAGGCATCA ACCTCAAATT TATGCACAGC
    CAGTTCCTGA AACTAAAGAA GTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48932
    Clone ID Related Accession (Same CDS sequence) XM_009303283.2 , XM_021477898.1
    Accession Version XM_009303283.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4875bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product MAP kinase-activating death domain protein isoform X20
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303283.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GTATTTGGGC TAAATTCTCT AATGGAGATT
    GTAACAGAGA TCGGCCCGGG GAGCGGAGAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGGAGCGGAT GGAGTAAAAC AACGTGTAGA AGGAGGAGAC
    ACTGAGGAGA AGAAATCACA GATCAGTGCA GACAGTGGCC TCAGTGTGAC ATCTGGCTCA
    CAGAAGAGTG ATACGGATTC CCTGGCGAGC TCTGAACCTC CTGCTCTGAC CAGAAGCACC
    AGCCAGGACT CAGAGGCCAG CACAGTGGTA AGTAACAGCT CAGGTGAGAC GCTCGGTGCA
    GACAGTGACC TGAGCAGCAC TGCTGGAGAT GGCCTGACAG GAAGACATGG TCAGCATCTC
    AACCTGTCCC GTGGCACACT GTCTGACAGT GAGATTGAGA CCAACCCTGC AACCAGCTCA
    GTGTTTGGCA AGACACACAA GCTGAAGCCA GGAGTGAAGG AGCCTGTTGG TGTTAATAAA
    GCAGCACCTG CTCCACCCGT TGAGGATGTT AGCATGAGGA TCTATCTGTG TGAGGGGCTT
    CTGGGTAAAG AGCGCTCAAC TCTGTGGGAT CAGATGCAGT TCTGGGAGGA CGCCTTCCTG
    GATGCCGTAA TGCTGGAACG GGAAGGAATG GGAATGGATC AGGGACCACA AGAAATGATT
    GACAGGTATC TTTCTCTGGG AGATCATGAC CGCAAGAGAC TGGAGGATGA TGAGGACAGA
    CTGCTGGCCA CGCTTCTGCA CAATATGATT GCTTACATGC TTATGATGAA GGTCGGTAAA
    AACGACATCA GGAAGAAAGT ACGTCGTCTT ATGGGCAAAT CCCACATTGG CCTCACTCAC
    AGTCAGGAGA TCAATGAAGT CCTGGATCGC CTCGCACATT TAAGCGGTCG GGAGCTTTCT
    ATCAGGCCGA GTGGCAGCAG GCATATTAAA AAACAGACAT TTGTTGTGCA TGCTGGGACA
    GATACAACTG GTGACATATT CTTCATGGAG GTGTGTGATG ATTGCATCGT CTTGCGCAGT
    AATATTGGCA CAGTCTATGA GCGCTGGTGG TATGAGAAGC TCATAAATAT GACTTACTGT
    CCCAAAACTA AAGTGTTGTG TCTGTGGAGG AGAAACGGCC AGGAGACGCA GCTAAACAAG
    TTTTACACTA AGAAGTGTCG GGAATTGTAC TACTGTGTTA AAGACAGCAT GGAGCGTGCT
    GCTGCTCGAC AACAAAGCAT CAAACCAGGT CCAGAGCTTG GAGGCGAGTT TCCGGTTCAG
    GACATGAAGA CAGGGGAGGG TGGTCTCCTC CAAGTCACAC TGGAAGGCAT CAACCTCAAA
    TTTATGCACA GCCAGGAGCG GAAGGTTTTC ATAGAGCTGA ATCACATTAA AAAGTGCAAT
    ACAGTGAAAG GAGTCTTTGT CCTGGAGGAG TTTGTTCCTG AAACTAAAGA AGTGGTGATC
    CATAAGTACA AGACTCCTAT GGCACATCAG ATCTGTTACT CGGTGCTCTG CCTCTTCTCC
    TACATGGCGG CGGTCAAGGG GAAAGAGTCA GAGGGCAAAC CCAAGATGCT GTCCCCTCGT
    CCACTAGCGA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301558.1
    CDS435..5309
    Translation

    Target ORF information:

    RefSeq Version XM_009303283.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303283.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GTATTTGGGC TAAATTCTCT AATGGAGATT
    GTAACAGAGA TCGGCCCGGG GAGCGGAGAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGGAGCGGAT GGAGTAAAAC AACGTGTAGA AGGAGGAGAC
    ACTGAGGAGA AGAAATCACA GATCAGTGCA GACAGTGGCC TCAGTGTGAC ATCTGGCTCA
    CAGAAGAGTG ATACGGATTC CCTGGCGAGC TCTGAACCTC CTGCTCTGAC CAGAAGCACC
    AGCCAGGACT CAGAGGCCAG CACAGTGGTA AGTAACAGCT CAGGTGAGAC GCTCGGTGCA
    GACAGTGACC TGAGCAGCAC TGCTGGAGAT GGCCTGACAG GAAGACATGG TCAGCATCTC
    AACCTGTCCC GTGGCACACT GTCTGACAGT GAGATTGAGA CCAACCCTGC AACCAGCTCA
    GTGTTTGGCA AGACACACAA GCTGAAGCCA GGAGTGAAGG AGCCTGTTGG TGTTAATAAA
    GCAGCACCTG CTCCACCCGT TGAGGATGTT AGCATGAGGA TCTATCTGTG TGAGGGGCTT
    CTGGGTAAAG AGCGCTCAAC TCTGTGGGAT CAGATGCAGT TCTGGGAGGA CGCCTTCCTG
    GATGCCGTAA TGCTGGAACG GGAAGGAATG GGAATGGATC AGGGACCACA AGAAATGATT
    GACAGGTATC TTTCTCTGGG AGATCATGAC CGCAAGAGAC TGGAGGATGA TGAGGACAGA
    CTGCTGGCCA CGCTTCTGCA CAATATGATT GCTTACATGC TTATGATGAA GGTCGGTAAA
    AACGACATCA GGAAGAAAGT ACGTCGTCTT ATGGGCAAAT CCCACATTGG CCTCACTCAC
    AGTCAGGAGA TCAATGAAGT CCTGGATCGC CTCGCACATT TAAGCGGTCG GGAGCTTTCT
    ATCAGGCCGA GTGGCAGCAG GCATATTAAA AAACAGACAT TTGTTGTGCA TGCTGGGACA
    GATACAACTG GTGACATATT CTTCATGGAG GTGTGTGATG ATTGCATCGT CTTGCGCAGT
    AATATTGGCA CAGTCTATGA GCGCTGGTGG TATGAGAAGC TCATAAATAT GACTTACTGT
    CCCAAAACTA AAGTGTTGTG TCTGTGGAGG AGAAACGGCC AGGAGACGCA GCTAAACAAG
    TTTTACACTA AGAAGTGTCG GGAATTGTAC TACTGTGTTA AAGACAGCAT GGAGCGTGCT
    GCTGCTCGAC AACAAAGCAT CAAACCAGGT CCAGAGCTTG GAGGCGAGTT TCCGGTTCAG
    GACATGAAGA CAGGGGAGGG TGGTCTCCTC CAAGTCACAC TGGAAGGCAT CAACCTCAAA
    TTTATGCACA GCCAGGAGCG GAAGGTTTTC ATAGAGCTGA ATCACATTAA AAAGTGCAAT
    ACAGTGAAAG GAGTCTTTGT CCTGGAGGAG TTTGTTCCTG AAACTAAAGA AGTGGTGATC
    CATAAGTACA AGACTCCTAT GGCACATCAG ATCTGTTACT CGGTGCTCTG CCTCTTCTCC
    TACATGGCGG CGGTCAAGGG GAAAGAGTCA GAGGGCAAAC CCAAGATGCT GTCCCCTCGT
    CCACTAGCGA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48932
    Clone ID Related Accession (Same CDS sequence) XM_009303283.2 , XM_021477898.1
    Accession Version XM_021477898.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4875bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X24
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GTATTTGGGC TAAATTCTCT AATGGAGATT
    GTAACAGAGA TCGGCCCGGG GAGCGGAGAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGGAGCGGAT GGAGTAAAAC AACGTGTAGA AGGAGGAGAC
    ACTGAGGAGA AGAAATCACA GATCAGTGCA GACAGTGGCC TCAGTGTGAC ATCTGGCTCA
    CAGAAGAGTG ATACGGATTC CCTGGCGAGC TCTGAACCTC CTGCTCTGAC CAGAAGCACC
    AGCCAGGACT CAGAGGCCAG CACAGTGGTA AGTAACAGCT CAGGTGAGAC GCTCGGTGCA
    GACAGTGACC TGAGCAGCAC TGCTGGAGAT GGCCTGACAG GAAGACATGG TCAGCATCTC
    AACCTGTCCC GTGGCACACT GTCTGACAGT GAGATTGAGA CCAACCCTGC AACCAGCTCA
    GTGTTTGGCA AGACACACAA GCTGAAGCCA GGAGTGAAGG AGCCTGTTGG TGTTAATAAA
    GCAGCACCTG CTCCACCCGT TGAGGATGTT AGCATGAGGA TCTATCTGTG TGAGGGGCTT
    CTGGGTAAAG AGCGCTCAAC TCTGTGGGAT CAGATGCAGT TCTGGGAGGA CGCCTTCCTG
    GATGCCGTAA TGCTGGAACG GGAAGGAATG GGAATGGATC AGGGACCACA AGAAATGATT
    GACAGGTATC TTTCTCTGGG AGATCATGAC CGCAAGAGAC TGGAGGATGA TGAGGACAGA
    CTGCTGGCCA CGCTTCTGCA CAATATGATT GCTTACATGC TTATGATGAA GGTCGGTAAA
    AACGACATCA GGAAGAAAGT ACGTCGTCTT ATGGGCAAAT CCCACATTGG CCTCACTCAC
    AGTCAGGAGA TCAATGAAGT CCTGGATCGC CTCGCACATT TAAGCGGTCG GGAGCTTTCT
    ATCAGGCCGA GTGGCAGCAG GCATATTAAA AAACAGACAT TTGTTGTGCA TGCTGGGACA
    GATACAACTG GTGACATATT CTTCATGGAG GTGTGTGATG ATTGCATCGT CTTGCGCAGT
    AATATTGGCA CAGTCTATGA GCGCTGGTGG TATGAGAAGC TCATAAATAT GACTTACTGT
    CCCAAAACTA AAGTGTTGTG TCTGTGGAGG AGAAACGGCC AGGAGACGCA GCTAAACAAG
    TTTTACACTA AGAAGTGTCG GGAATTGTAC TACTGTGTTA AAGACAGCAT GGAGCGTGCT
    GCTGCTCGAC AACAAAGCAT CAAACCAGGT CCAGAGCTTG GAGGCGAGTT TCCGGTTCAG
    GACATGAAGA CAGGGGAGGG TGGTCTCCTC CAAGTCACAC TGGAAGGCAT CAACCTCAAA
    TTTATGCACA GCCAGGAGCG GAAGGTTTTC ATAGAGCTGA ATCACATTAA AAAGTGCAAT
    ACAGTGAAAG GAGTCTTTGT CCTGGAGGAG TTTGTTCCTG AAACTAAAGA AGTGGTGATC
    CATAAGTACA AGACTCCTAT GGCACATCAG ATCTGTTACT CGGTGCTCTG CCTCTTCTCC
    TACATGGCGG CGGTCAAGGG GAAAGAGTCA GAGGGCAAAC CCAAGATGCT GTCCCCTCGT
    CCACTAGCGA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333573.1
    CDS1..4875
    Translation

    Target ORF information:

    RefSeq Version XM_021477898.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021477898.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GTATTTGGGC TAAATTCTCT AATGGAGATT
    GTAACAGAGA TCGGCCCGGG GAGCGGAGAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGGAGCGGAT GGAGTAAAAC AACGTGTAGA AGGAGGAGAC
    ACTGAGGAGA AGAAATCACA GATCAGTGCA GACAGTGGCC TCAGTGTGAC ATCTGGCTCA
    CAGAAGAGTG ATACGGATTC CCTGGCGAGC TCTGAACCTC CTGCTCTGAC CAGAAGCACC
    AGCCAGGACT CAGAGGCCAG CACAGTGGTA AGTAACAGCT CAGGTGAGAC GCTCGGTGCA
    GACAGTGACC TGAGCAGCAC TGCTGGAGAT GGCCTGACAG GAAGACATGG TCAGCATCTC
    AACCTGTCCC GTGGCACACT GTCTGACAGT GAGATTGAGA CCAACCCTGC AACCAGCTCA
    GTGTTTGGCA AGACACACAA GCTGAAGCCA GGAGTGAAGG AGCCTGTTGG TGTTAATAAA
    GCAGCACCTG CTCCACCCGT TGAGGATGTT AGCATGAGGA TCTATCTGTG TGAGGGGCTT
    CTGGGTAAAG AGCGCTCAAC TCTGTGGGAT CAGATGCAGT TCTGGGAGGA CGCCTTCCTG
    GATGCCGTAA TGCTGGAACG GGAAGGAATG GGAATGGATC AGGGACCACA AGAAATGATT
    GACAGGTATC TTTCTCTGGG AGATCATGAC CGCAAGAGAC TGGAGGATGA TGAGGACAGA
    CTGCTGGCCA CGCTTCTGCA CAATATGATT GCTTACATGC TTATGATGAA GGTCGGTAAA
    AACGACATCA GGAAGAAAGT ACGTCGTCTT ATGGGCAAAT CCCACATTGG CCTCACTCAC
    AGTCAGGAGA TCAATGAAGT CCTGGATCGC CTCGCACATT TAAGCGGTCG GGAGCTTTCT
    ATCAGGCCGA GTGGCAGCAG GCATATTAAA AAACAGACAT TTGTTGTGCA TGCTGGGACA
    GATACAACTG GTGACATATT CTTCATGGAG GTGTGTGATG ATTGCATCGT CTTGCGCAGT
    AATATTGGCA CAGTCTATGA GCGCTGGTGG TATGAGAAGC TCATAAATAT GACTTACTGT
    CCCAAAACTA AAGTGTTGTG TCTGTGGAGG AGAAACGGCC AGGAGACGCA GCTAAACAAG
    TTTTACACTA AGAAGTGTCG GGAATTGTAC TACTGTGTTA AAGACAGCAT GGAGCGTGCT
    GCTGCTCGAC AACAAAGCAT CAAACCAGGT CCAGAGCTTG GAGGCGAGTT TCCGGTTCAG
    GACATGAAGA CAGGGGAGGG TGGTCTCCTC CAAGTCACAC TGGAAGGCAT CAACCTCAAA
    TTTATGCACA GCCAGGAGCG GAAGGTTTTC ATAGAGCTGA ATCACATTAA AAAGTGCAAT
    ACAGTGAAAG GAGTCTTTGT CCTGGAGGAG TTTGTTCCTG AAACTAAAGA AGTGGTGATC
    CATAAGTACA AGACTCCTAT GGCACATCAG ATCTGTTACT CGGTGCTCTG CCTCTTCTCC
    TACATGGCGG CGGTCAAGGG GAAAGAGTCA GAGGGCAAAC CCAAGATGCT GTCCCCTCGT
    CCACTAGCGA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48933
    Clone ID Related Accession (Same CDS sequence) XM_009303284.2
    Accession Version XM_009303284.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4872bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product MAP kinase-activating death domain protein isoform X21
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303284.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGTG TCTGGTAAGG
    TTGACTCTGA TCACCCAAAA GCAGATCTTC GCCTCCGATT CTAAGGCTCT GGCTAGCATG
    AGTCTAAACA CACAGCCTAT TCTGAACCTG GAGAAGTTTC AGGATGGACA GGAGTTGTCT
    TTATTGCCCC CCGGTCGTGA CAAAGCTTCC CCGTCCTCCA CTGAGTTCAA CCCACTCATC
    TACGGCAATG ATGTGGACTC AGTTGATGTT GCCACAAGGG TGGCAATGGT GAGGTTCTTC
    AACTCTCCAA ACGTGCTGCA AGGGTTCCAG ATGCACACAC GCACTCTCCG TCTCTTCCCT
    CGTCCTGTGG TGGCCTTTCA GTCCACATCT TTCCTGGCTT CTAGGCCACG ACGAAATGGA
    TTCACAGAAA AGCTCTCCCA CACTCAGGCA GTGGAGTACT ATGGAGAATG GGCTCTCAAC
    CCCACCAACC TGGCTTTCCA AAGGATTCAT AACAATGTGT ATGACCCCTC TCTAATAGGC
    GATAAGCCTA AGTGGTATGC CCACCAGTTA CAGCCAGTAT TCTACCGCGT GTATGATGGA
    AACTCTCATT TAGCGGAGGC CTTGAGTGGA CCCCTGCAGG ATGAGACCAA TGATTCAGAC
    CCAACAGACG ACAGTGGCAG CGACAGTGAA GCATATGATG ACTCCAGCTC CTCCTACTCA
    TCTCTTGGAG ATTTTGTGAA TGAGATGATC AAAGGGGACA TACAGGGAGA CACACCAAAT
    GTTGATCCCC TGACCCATGC TGCACTGGGA GATGCAGATG AGGTTGAGAT CCATGACTTT
    CACGAGTATA AGGGGAACAG CGGTGAACCA GAGCCAGAAG GCCCCACAGA AGCAGCGGAC
    AGTCAGCCCT TGCGGTCCAG CTCTAGCACC ACAGCCAGTT CCAGCCCCAG TACAGTCATA
    CAGGGAGTCA ACCATGAGCA GAAGGAACCT GTTGAGGTGG AGCCCATAGC AAATGTGACT
    TACCCAAGCT CTGCCCCAGT ATTGGGTCCT CCTCCTTTTA CAAGACCCAC CCCTGATCCA
    ACCCTTGTTG ACCCTGCCAA TAAGAAGCGA GAATATGACA ACCCCTATTT TGAGCCCCAG
    TACGGTTTCC CTACAGAGGA AGATGCTGAG ACTGATGAGC AGGAGGAGAG TTACACACCA
    CGATTTAACC AGAATCTCAA TGGCAATAAG CCTTCTCGTC CACTGAGACC CAGCAGCCTG
    AAGCTTCCGG GTGAATCTGA CGGAGAGGGA GACTCCAGAA ATAGCTCTCC TAACTCAACC
    ATCTCCAACA ATAGCAGTGA TGGCTTTGGG GGACTCATGT CCTTTGCCAG TAACTTGTAT
    AAAAACCATG GCACCAGTTT CAGTCTGTCC AGCCTGGCTC TGCCTAATAA AGCGAGAGAG
    AAGAACACGC CGTTTCCCAG CCTCAAAGGT GCCCGAGCCC CCAGAGCACT GGTAGATCAG
    AAATCCTCTG TTATTAAGCA CAGCCCTACT GTGAAGAGAG AGTCTCCGTC TCCTCAAGGC
    AGAGCCAACA ATACCAGTGA GAACCAGCAG TTTCTAAAGG AGGTGGTCCA GAGCGTGCTG
    GAGGGGCAGG GTGTGGGCTG GCTTAACATG AAGAAGGTGC GTCGCCTGCT AGAGAATGAA
    CAGCTCAGAG TCTTTGTGCT CAGCAAACTC AACCGTGCGG TCCAGTCAGA GGAGGACGCC
    CAGCAGGAAG TTATAAGAGA TGTGGAGATA AATAGAAAGG TCTACAAAGG CATGCTGGAT
    ATTTTGAAGT GCACGGTGTC CAGTTTGGAG CATTCATACA CTAATGCTGG CTTAGGGGGC
    ATGGCCAGTG TCTTCAGTCT GCTAGAGATA GCACGCACCC ACTACCAAAC CAAAGATCCA
    GAGAAGCGGA AACGGAGCCC CACCGAAGGG CTTAGCAGTC CAGGCAGTAA GGAGAGCCCA
    TCGGGCCGAA TGGAGAGTGC CAGGGCGGCA GGGGTGCTTC TGGTACCCCG TATCCAATTG
    CCGCCACCCT CCTCTGGGAA ATCATCACGC CAGTTTGATA CCCGGAGTCT GAACGAGGAG
    AATTTTATTG CCTCCATTGG AGCGGATGGA GTAAAACAAC GTGTAGAAGG AGGAGACACT
    GAGGAGAAGA AATCACAGAT CAGTGCAGAC AGTGGCCTCA GTGTGACATC TGGCTCACAG
    AAGAGTGATA CGGATTCCCT GGCGAGCTCT GAACCTCCTG CTCTGACCAG AAGCACCAGC
    CAGGACTCAG AGGCCAGCAC AGTGGTAAGT AACAGCTCAG GTGAGACGCT CGGTGCAGAC
    AGTGACCTGA GCAGCACTGC TGGAGATGGC CTGACAGGAA GACATGGTCA GCATCTCAAC
    CTGTCCCGTG GCACACTGTC TGACAGTGAG ATTGAGACCA ACCCTGCAAC CAGCTCAGTG
    TTTGGCAAGA CACACAAGCT GAAGCCAGGA GTGAAGGAGC CTGTTGGTGT TAATAAAGCA
    GCACCTGCTC CACCCGTTGA GGATGTTAGC ATGAGGATCT ATCTGTGTGA GGGGCTTCTG
    GGTAAAGAGC GCTCAACTCT GTGGGATCAG ATGCAGTTCT GGGAGGACGC CTTCCTGGAT
    GCCGTAATGC TGGAACGGGA AGGAATGGGA ATGGATCAGG GACCACAAGA AATGATTGAC
    AGGTATCTTT CTCTGGGAGA TCATGACCGC AAGAGACTGG AGGATGATGA GGACAGACTG
    CTGGCCACGC TTCTGCACAA TATGATTGCT TACATGCTTA TGATGAAGGT CGGTAAAAAC
    GACATCAGGA AGAAAGTACG TCGTCTTATG GGCAAATCCC ACATTGGCCT CACTCACAGT
    CAGGAGATCA ATGAAGTCCT GGATCGCCTC GCACATTTAA GCGGTCGGGA GCTTTCTATC
    AGGCCGAGTG GCAGCAGGCA TATTAAAAAA CAGACATTTG TTGTGCATGC TGGGACAGAT
    ACAACTGGTG ACATATTCTT CATGGAGGTG TGTGATGATT GCATCGTCTT GCGCAGTAAT
    ATTGGCACAG TCTATGAGCG CTGGTGGTAT GAGAAGCTCA TAAATATGAC TTACTGTCCC
    AAAACTAAAG TGTTGTGTCT GTGGAGGAGA AACGGCCAGG AGACGCAGCT AAACAAGTTT
    TACACTAAGA AGTGTCGGGA ATTGTACTAC TGTGTTAAAG ACAGCATGGA GCGTGCTGCT
    GCTCGACAAC AAAGCATCAA ACCAGGTCCA GAGCTTGGAG GCGAGTTTCC GGTTCAGGAC
    ATGAAGACAG GGGAGGGTGG TCTCCTCCAA GTCACACTGG AAGGCATCAA CCTCAAATTT
    ATGCACAGCC AGGAGCGGAA GGTTTTCATA GAGCTGAATC ACATTAAAAA GTGCAATACA
    GTGAAAGGAG TCTTTGTCCT GGAGGAGTTT GTTCCTGAAA CTAAAGAAGT GGTGATCCAT
    AAGTACAAGA CTCCTATGGC ACATCAGATC TGTTACTCGG TGCTCTGCCT CTTCTCCTAC
    ATGGCGGCGG TCAAGGGGAA AGAGTCAGAG GGCAAACCCA AGATGCTGTC CCCTCGTCCA
    CTAGCGAGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301559.1
    CDS435..5306
    Translation

    Target ORF information:

    RefSeq Version XM_009303284.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303284.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGTG TCTGGTAAGG
    TTGACTCTGA TCACCCAAAA GCAGATCTTC GCCTCCGATT CTAAGGCTCT GGCTAGCATG
    AGTCTAAACA CACAGCCTAT TCTGAACCTG GAGAAGTTTC AGGATGGACA GGAGTTGTCT
    TTATTGCCCC CCGGTCGTGA CAAAGCTTCC CCGTCCTCCA CTGAGTTCAA CCCACTCATC
    TACGGCAATG ATGTGGACTC AGTTGATGTT GCCACAAGGG TGGCAATGGT GAGGTTCTTC
    AACTCTCCAA ACGTGCTGCA AGGGTTCCAG ATGCACACAC GCACTCTCCG TCTCTTCCCT
    CGTCCTGTGG TGGCCTTTCA GTCCACATCT TTCCTGGCTT CTAGGCCACG ACGAAATGGA
    TTCACAGAAA AGCTCTCCCA CACTCAGGCA GTGGAGTACT ATGGAGAATG GGCTCTCAAC
    CCCACCAACC TGGCTTTCCA AAGGATTCAT AACAATGTGT ATGACCCCTC TCTAATAGGC
    GATAAGCCTA AGTGGTATGC CCACCAGTTA CAGCCAGTAT TCTACCGCGT GTATGATGGA
    AACTCTCATT TAGCGGAGGC CTTGAGTGGA CCCCTGCAGG ATGAGACCAA TGATTCAGAC
    CCAACAGACG ACAGTGGCAG CGACAGTGAA GCATATGATG ACTCCAGCTC CTCCTACTCA
    TCTCTTGGAG ATTTTGTGAA TGAGATGATC AAAGGGGACA TACAGGGAGA CACACCAAAT
    GTTGATCCCC TGACCCATGC TGCACTGGGA GATGCAGATG AGGTTGAGAT CCATGACTTT
    CACGAGTATA AGGGGAACAG CGGTGAACCA GAGCCAGAAG GCCCCACAGA AGCAGCGGAC
    AGTCAGCCCT TGCGGTCCAG CTCTAGCACC ACAGCCAGTT CCAGCCCCAG TACAGTCATA
    CAGGGAGTCA ACCATGAGCA GAAGGAACCT GTTGAGGTGG AGCCCATAGC AAATGTGACT
    TACCCAAGCT CTGCCCCAGT ATTGGGTCCT CCTCCTTTTA CAAGACCCAC CCCTGATCCA
    ACCCTTGTTG ACCCTGCCAA TAAGAAGCGA GAATATGACA ACCCCTATTT TGAGCCCCAG
    TACGGTTTCC CTACAGAGGA AGATGCTGAG ACTGATGAGC AGGAGGAGAG TTACACACCA
    CGATTTAACC AGAATCTCAA TGGCAATAAG CCTTCTCGTC CACTGAGACC CAGCAGCCTG
    AAGCTTCCGG GTGAATCTGA CGGAGAGGGA GACTCCAGAA ATAGCTCTCC TAACTCAACC
    ATCTCCAACA ATAGCAGTGA TGGCTTTGGG GGACTCATGT CCTTTGCCAG TAACTTGTAT
    AAAAACCATG GCACCAGTTT CAGTCTGTCC AGCCTGGCTC TGCCTAATAA AGCGAGAGAG
    AAGAACACGC CGTTTCCCAG CCTCAAAGGT GCCCGAGCCC CCAGAGCACT GGTAGATCAG
    AAATCCTCTG TTATTAAGCA CAGCCCTACT GTGAAGAGAG AGTCTCCGTC TCCTCAAGGC
    AGAGCCAACA ATACCAGTGA GAACCAGCAG TTTCTAAAGG AGGTGGTCCA GAGCGTGCTG
    GAGGGGCAGG GTGTGGGCTG GCTTAACATG AAGAAGGTGC GTCGCCTGCT AGAGAATGAA
    CAGCTCAGAG TCTTTGTGCT CAGCAAACTC AACCGTGCGG TCCAGTCAGA GGAGGACGCC
    CAGCAGGAAG TTATAAGAGA TGTGGAGATA AATAGAAAGG TCTACAAAGG CATGCTGGAT
    ATTTTGAAGT GCACGGTGTC CAGTTTGGAG CATTCATACA CTAATGCTGG CTTAGGGGGC
    ATGGCCAGTG TCTTCAGTCT GCTAGAGATA GCACGCACCC ACTACCAAAC CAAAGATCCA
    GAGAAGCGGA AACGGAGCCC CACCGAAGGG CTTAGCAGTC CAGGCAGTAA GGAGAGCCCA
    TCGGGCCGAA TGGAGAGTGC CAGGGCGGCA GGGGTGCTTC TGGTACCCCG TATCCAATTG
    CCGCCACCCT CCTCTGGGAA ATCATCACGC CAGTTTGATA CCCGGAGTCT GAACGAGGAG
    AATTTTATTG CCTCCATTGG AGCGGATGGA GTAAAACAAC GTGTAGAAGG AGGAGACACT
    GAGGAGAAGA AATCACAGAT CAGTGCAGAC AGTGGCCTCA GTGTGACATC TGGCTCACAG
    AAGAGTGATA CGGATTCCCT GGCGAGCTCT GAACCTCCTG CTCTGACCAG AAGCACCAGC
    CAGGACTCAG AGGCCAGCAC AGTGGTAAGT AACAGCTCAG GTGAGACGCT CGGTGCAGAC
    AGTGACCTGA GCAGCACTGC TGGAGATGGC CTGACAGGAA GACATGGTCA GCATCTCAAC
    CTGTCCCGTG GCACACTGTC TGACAGTGAG ATTGAGACCA ACCCTGCAAC CAGCTCAGTG
    TTTGGCAAGA CACACAAGCT GAAGCCAGGA GTGAAGGAGC CTGTTGGTGT TAATAAAGCA
    GCACCTGCTC CACCCGTTGA GGATGTTAGC ATGAGGATCT ATCTGTGTGA GGGGCTTCTG
    GGTAAAGAGC GCTCAACTCT GTGGGATCAG ATGCAGTTCT GGGAGGACGC CTTCCTGGAT
    GCCGTAATGC TGGAACGGGA AGGAATGGGA ATGGATCAGG GACCACAAGA AATGATTGAC
    AGGTATCTTT CTCTGGGAGA TCATGACCGC AAGAGACTGG AGGATGATGA GGACAGACTG
    CTGGCCACGC TTCTGCACAA TATGATTGCT TACATGCTTA TGATGAAGGT CGGTAAAAAC
    GACATCAGGA AGAAAGTACG TCGTCTTATG GGCAAATCCC ACATTGGCCT CACTCACAGT
    CAGGAGATCA ATGAAGTCCT GGATCGCCTC GCACATTTAA GCGGTCGGGA GCTTTCTATC
    AGGCCGAGTG GCAGCAGGCA TATTAAAAAA CAGACATTTG TTGTGCATGC TGGGACAGAT
    ACAACTGGTG ACATATTCTT CATGGAGGTG TGTGATGATT GCATCGTCTT GCGCAGTAAT
    ATTGGCACAG TCTATGAGCG CTGGTGGTAT GAGAAGCTCA TAAATATGAC TTACTGTCCC
    AAAACTAAAG TGTTGTGTCT GTGGAGGAGA AACGGCCAGG AGACGCAGCT AAACAAGTTT
    TACACTAAGA AGTGTCGGGA ATTGTACTAC TGTGTTAAAG ACAGCATGGA GCGTGCTGCT
    GCTCGACAAC AAAGCATCAA ACCAGGTCCA GAGCTTGGAG GCGAGTTTCC GGTTCAGGAC
    ATGAAGACAG GGGAGGGTGG TCTCCTCCAA GTCACACTGG AAGGCATCAA CCTCAAATTT
    ATGCACAGCC AGGAGCGGAA GGTTTTCATA GAGCTGAATC ACATTAAAAA GTGCAATACA
    GTGAAAGGAG TCTTTGTCCT GGAGGAGTTT GTTCCTGAAA CTAAAGAAGT GGTGATCCAT
    AAGTACAAGA CTCCTATGGC ACATCAGATC TGTTACTCGG TGCTCTGCCT CTTCTCCTAC
    ATGGCGGCGG TCAAGGGGAA AGAGTCAGAG GGCAAACCCA AGATGCTGTC CCCTCGTCCA
    CTAGCGAGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48934
    Clone ID Related Accession (Same CDS sequence) XM_009303286.2
    Accession Version XM_009303286.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4863bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product MAP kinase-activating death domain protein isoform X23
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303286.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGTG TCTGGTAAGG
    TTGACTCTGA TCACCCAAAA GCAGATCTTC GCCTCCGATT CTAAGGCTCT GGCTAGCATG
    AGTCTAAACA CACAGCCTAT TCTGAACCTG GAGAAGTTTC AGGATGGACA GGAGTTGTCT
    TTATTGCCCC CCGGTCGTGA CAAAGCTTCC CCGTCCTCCA CTGAGTTCAA CCCACTCATC
    TACGGCAATG ATGTGGACTC AGTTGATGTT GCCACAAGGG TGGCAATGGT GAGGTTCTTC
    AACTCTCCAA ACGTGCTGCA AGGGTTCCAG ATGCACACAC GCACTCTCCG TCTCTTCCCT
    CGTCCTGTGG TGGCCTTTCA GTCCACATCT TTCCTGGCTT CTAGGCCACG ACGAAATGGA
    TTCACAGAAA AGCTCTCCCA CACTCAGGCA GTGGAGTACT ATGGAGAATG GGCTCTCAAC
    CCCACCAACC TGGCTTTCCA AAGGATTCAT AACAATGTGT ATGACCCCTC TCTAATAGGC
    GATAAGCCTA AGTGGTATGC CCACCAGTTA CAGCCAGTAT TCTACCGCGT GTATGATGGA
    AACTCTCATT TAGCGGAGGC CTTGAGTGGA CCCCTGCAGG ATGAGACCAA TGATTCAGAC
    CCAACAGACG ACAGTGGCAG CGACAGTGAA GCATATGATG ACTCCAGCTC CTCCTACTCA
    TCTCTTGGAG ATTTTGTGAA TGAGATGATC AAAGGGGACA TACAGGGAGA CACACCAAAT
    GTTGATCCCC TGACCCATGC TGCACTGGGA GATGCAGATG AGGTTGAGAT CCATGACTTT
    CACGAGTATA AGGGGAACAG CGGTGAACCA GAGCCAGAAG GCCCCACAGA AGCAGCGGAC
    AGTCAGCCCT TGCGGTCCAG CTCTAGCACC ACAGCCAGTT CCAGCCCCAG TACAGTCATA
    CAGGGAGTCA ACCATGAGCA GAAGGAACCT GTTGAGGTGG AGCCCATAGC AAATGTGACT
    TACCCAAGCT CTGCCCCAGT ATTGGGTCCT CCTCCTTTTA CAAGACCCAC CCCTGATCCA
    ACCCTTGTTG ACCCTGCCAA TAAGAAGCGA GAATATGACA ACCCCTATTT TGAGCCCCAG
    TACGGTTTCC CTACAGAGGA AGATGCTGAG ACTGATGAGC AGGAGGAGAG TTACACACCA
    CGATTTAACC AGAATCTCAA TGGCAATAAG CCTTCTCGTC CACTGAGACC CAGCAGCCTG
    AAGCTTCCGG GTGAATCTGA CGGAGAGGGA GACTCCAGAA ATAGCTCTCC TAACTCAACC
    ATCTCCAACA ATAGCAGTGA TGGCTTTGGG GGACTCATGT CCTTTGCCAG TAACTTGTAT
    AAAAACCATG GCACCAGTTT CAGTCTGTCC AGCCTGGCTC TGCCTAATAA AGCGAGAGAG
    AAGAACACGC CGTTTCCCAG CCTCAAAGGT GCCCGAGCCC CCAGAGCACT GGTAGATCAG
    AAATCCTCTG TTATTAAGCA CAGCCCTACT GTGAAGAGAG AGTCTCCGTC TCCTCAAGGC
    AGAGCCAACA ATACCAGTGA GAACCAGCAG TTTCTAAAGG AGGTGGTCCA GAGCGTGCTG
    GAGGGGCAGG GTGTGGGCTG GCTTAACATG AAGAAGGTGC GTCGCCTGCT AGAGAATGAA
    CAGCTCAGAG TCTTTGTGCT CAGCAAACTC AACCGTGCGG TCCAGTCAGA GGAGGACGCC
    CAGCAGGAAG TTATAAGAGA TGTGGAGATA AATAGAAAGG TCTACAAAGG CATGCTGGAT
    ATTTTGAAGT GCACGGTGTC CAGTTTGGAG CATTCATACA CTAATGCTGG CTTAGGGGGC
    ATGGCCAGTG TCTTCAGTCT GCTAGAGATA GCACGCACCC ACTACCAAAC CAAAGATCCA
    GAGAAGCGGA AACGGAGCCC CACCGAAGGG CTTAGCAGTC CAGGCAGTAA GGAGAGCCCA
    TCGGGCCGAA TGGAGAGTGC CAGGGCGGCA GGGGTGCTTC TGGTACCCCG TATCCAATTG
    CCGCCACCCT CCTCTGGGAA ATCATCACGC CAGTTTGATA CCCGGAGTCT GAACGAGGAG
    AATTTTATTG CCTCCATTGG AGCGGATGGA GTAAAACAAC GTGTAGAAGG AGGAGACACT
    GAGGAGAAGA AATCACAGAT CAGTGCAGAC AGTGGCCTCA GTGTGACATC TGGCTCACAG
    AAGAGTGATA CGGATTCCCT GGCGAGCTCT GAACCTCCTG CTCTGACCAG AAGCACCAGC
    CAGGACTCAG AGGCCAGCAC AGTGGTAAGT AACAGCTCAG GTGAGACGCT CGGTGCAGAC
    AGTGACCTGA GCAGCACTGC TGGAGATGGC CTGACAGGAA GACATGGTCA GCATCTCAAC
    CTGTCCCGTG GCACACTGTC TGACAGTGAG ATTGAGACCA ACCCTGCAAC CAGCTCAGTG
    TTTGGCAAGA CACACAAGCT GAAGCCAGGA GTGAAGGAGC CTGTTGGTGT TAATAAAGCA
    GCACCTGCTC CACCCGTTGA GGATGTTAGC ATGAGGATCT ATCTGTGTGA GGGGCTTCTG
    GGTAAAGAGC GCTCAACTCT GTGGGATCAG ATGCAGTTCT GGGAGGACGC CTTCCTGGAT
    GCCGTAATGC TGGAACGGGA AGGAATGGGA ATGGATCAGG GACCACAAGA AATGATTGAC
    AGGTATCTTT CTCTGGGAGA TCATGACCGC AAGAGACTGG AGGATGATGA GGACAGACTG
    CTGGCCACGC TTCTGCACAA TATGATTGCT TACATGCTTA TGATGAAGGT CGGTAAAAAC
    GACATCAGGA AGAAAGTACG TCGTCTTATG GGCAAATCCC ACATTGGCCT CACTCACAGT
    CAGGAGATCA ATGAAGTCCT GGATCGCCTC GCACATTTAA GCGGTCGGGA GCTTTCTATC
    AGGCCGAGTG GCAGCAGGCA TATTAAAAAA CAGACATTTG TTGTGCATGC TGGGACAGAT
    ACAACTGGTG ACATATTCTT CATGGAGGTG TGTGATGATT GCATCGTCTT GCGCAGTAAT
    ATTGGCACAG TCTATGAGCG CTGGTGGTAT GAGAAGCTCA TAAATATGAC TTACTGTCCC
    AAAACTAAAG TGTTGTGTCT GTGGAGGAGA AACGGCCAGG AGACGCAGCT AAACAAGTTT
    TACACTAAGA AGTGTCGGGA ATTGTACTAC TGTGTTAAAG ACAGCATGGA GCGTGCTGCT
    GCTCGACAAC AAAGCATCAA ACCAGGTCCA GAGCTTGGAG GCGAGTTTCC GGTTCAGGAC
    ATGAAGACAG GGGAGGGTGG TCTCCTCCAA GTCACACTGG AAGGCATCAA CCTCAAATTT
    ATGCACAGCC AGGTTTTCAT AGAGCTGAAT CACATTAAAA AGTGCAATAC AGTGAAAGGA
    GTCTTTGTCC TGGAGGAGTT TGTTCCTGAA ACTAAAGAAG TGGTGATCCA TAAGTACAAG
    ACTCCTATGG CACATCAGAT CTGTTACTCG GTGCTCTGCC TCTTCTCCTA CATGGCGGCG
    GTCAAGGGGA AAGAGTCAGA GGGCAAACCC AAGATGCTGT CCCCTCGTCC ACTAGCGAGC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301561.1
    CDS435..5297
    Translation

    Target ORF information:

    RefSeq Version XM_009303286.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303286.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGTG TCTGGTAAGG
    TTGACTCTGA TCACCCAAAA GCAGATCTTC GCCTCCGATT CTAAGGCTCT GGCTAGCATG
    AGTCTAAACA CACAGCCTAT TCTGAACCTG GAGAAGTTTC AGGATGGACA GGAGTTGTCT
    TTATTGCCCC CCGGTCGTGA CAAAGCTTCC CCGTCCTCCA CTGAGTTCAA CCCACTCATC
    TACGGCAATG ATGTGGACTC AGTTGATGTT GCCACAAGGG TGGCAATGGT GAGGTTCTTC
    AACTCTCCAA ACGTGCTGCA AGGGTTCCAG ATGCACACAC GCACTCTCCG TCTCTTCCCT
    CGTCCTGTGG TGGCCTTTCA GTCCACATCT TTCCTGGCTT CTAGGCCACG ACGAAATGGA
    TTCACAGAAA AGCTCTCCCA CACTCAGGCA GTGGAGTACT ATGGAGAATG GGCTCTCAAC
    CCCACCAACC TGGCTTTCCA AAGGATTCAT AACAATGTGT ATGACCCCTC TCTAATAGGC
    GATAAGCCTA AGTGGTATGC CCACCAGTTA CAGCCAGTAT TCTACCGCGT GTATGATGGA
    AACTCTCATT TAGCGGAGGC CTTGAGTGGA CCCCTGCAGG ATGAGACCAA TGATTCAGAC
    CCAACAGACG ACAGTGGCAG CGACAGTGAA GCATATGATG ACTCCAGCTC CTCCTACTCA
    TCTCTTGGAG ATTTTGTGAA TGAGATGATC AAAGGGGACA TACAGGGAGA CACACCAAAT
    GTTGATCCCC TGACCCATGC TGCACTGGGA GATGCAGATG AGGTTGAGAT CCATGACTTT
    CACGAGTATA AGGGGAACAG CGGTGAACCA GAGCCAGAAG GCCCCACAGA AGCAGCGGAC
    AGTCAGCCCT TGCGGTCCAG CTCTAGCACC ACAGCCAGTT CCAGCCCCAG TACAGTCATA
    CAGGGAGTCA ACCATGAGCA GAAGGAACCT GTTGAGGTGG AGCCCATAGC AAATGTGACT
    TACCCAAGCT CTGCCCCAGT ATTGGGTCCT CCTCCTTTTA CAAGACCCAC CCCTGATCCA
    ACCCTTGTTG ACCCTGCCAA TAAGAAGCGA GAATATGACA ACCCCTATTT TGAGCCCCAG
    TACGGTTTCC CTACAGAGGA AGATGCTGAG ACTGATGAGC AGGAGGAGAG TTACACACCA
    CGATTTAACC AGAATCTCAA TGGCAATAAG CCTTCTCGTC CACTGAGACC CAGCAGCCTG
    AAGCTTCCGG GTGAATCTGA CGGAGAGGGA GACTCCAGAA ATAGCTCTCC TAACTCAACC
    ATCTCCAACA ATAGCAGTGA TGGCTTTGGG GGACTCATGT CCTTTGCCAG TAACTTGTAT
    AAAAACCATG GCACCAGTTT CAGTCTGTCC AGCCTGGCTC TGCCTAATAA AGCGAGAGAG
    AAGAACACGC CGTTTCCCAG CCTCAAAGGT GCCCGAGCCC CCAGAGCACT GGTAGATCAG
    AAATCCTCTG TTATTAAGCA CAGCCCTACT GTGAAGAGAG AGTCTCCGTC TCCTCAAGGC
    AGAGCCAACA ATACCAGTGA GAACCAGCAG TTTCTAAAGG AGGTGGTCCA GAGCGTGCTG
    GAGGGGCAGG GTGTGGGCTG GCTTAACATG AAGAAGGTGC GTCGCCTGCT AGAGAATGAA
    CAGCTCAGAG TCTTTGTGCT CAGCAAACTC AACCGTGCGG TCCAGTCAGA GGAGGACGCC
    CAGCAGGAAG TTATAAGAGA TGTGGAGATA AATAGAAAGG TCTACAAAGG CATGCTGGAT
    ATTTTGAAGT GCACGGTGTC CAGTTTGGAG CATTCATACA CTAATGCTGG CTTAGGGGGC
    ATGGCCAGTG TCTTCAGTCT GCTAGAGATA GCACGCACCC ACTACCAAAC CAAAGATCCA
    GAGAAGCGGA AACGGAGCCC CACCGAAGGG CTTAGCAGTC CAGGCAGTAA GGAGAGCCCA
    TCGGGCCGAA TGGAGAGTGC CAGGGCGGCA GGGGTGCTTC TGGTACCCCG TATCCAATTG
    CCGCCACCCT CCTCTGGGAA ATCATCACGC CAGTTTGATA CCCGGAGTCT GAACGAGGAG
    AATTTTATTG CCTCCATTGG AGCGGATGGA GTAAAACAAC GTGTAGAAGG AGGAGACACT
    GAGGAGAAGA AATCACAGAT CAGTGCAGAC AGTGGCCTCA GTGTGACATC TGGCTCACAG
    AAGAGTGATA CGGATTCCCT GGCGAGCTCT GAACCTCCTG CTCTGACCAG AAGCACCAGC
    CAGGACTCAG AGGCCAGCAC AGTGGTAAGT AACAGCTCAG GTGAGACGCT CGGTGCAGAC
    AGTGACCTGA GCAGCACTGC TGGAGATGGC CTGACAGGAA GACATGGTCA GCATCTCAAC
    CTGTCCCGTG GCACACTGTC TGACAGTGAG ATTGAGACCA ACCCTGCAAC CAGCTCAGTG
    TTTGGCAAGA CACACAAGCT GAAGCCAGGA GTGAAGGAGC CTGTTGGTGT TAATAAAGCA
    GCACCTGCTC CACCCGTTGA GGATGTTAGC ATGAGGATCT ATCTGTGTGA GGGGCTTCTG
    GGTAAAGAGC GCTCAACTCT GTGGGATCAG ATGCAGTTCT GGGAGGACGC CTTCCTGGAT
    GCCGTAATGC TGGAACGGGA AGGAATGGGA ATGGATCAGG GACCACAAGA AATGATTGAC
    AGGTATCTTT CTCTGGGAGA TCATGACCGC AAGAGACTGG AGGATGATGA GGACAGACTG
    CTGGCCACGC TTCTGCACAA TATGATTGCT TACATGCTTA TGATGAAGGT CGGTAAAAAC
    GACATCAGGA AGAAAGTACG TCGTCTTATG GGCAAATCCC ACATTGGCCT CACTCACAGT
    CAGGAGATCA ATGAAGTCCT GGATCGCCTC GCACATTTAA GCGGTCGGGA GCTTTCTATC
    AGGCCGAGTG GCAGCAGGCA TATTAAAAAA CAGACATTTG TTGTGCATGC TGGGACAGAT
    ACAACTGGTG ACATATTCTT CATGGAGGTG TGTGATGATT GCATCGTCTT GCGCAGTAAT
    ATTGGCACAG TCTATGAGCG CTGGTGGTAT GAGAAGCTCA TAAATATGAC TTACTGTCCC
    AAAACTAAAG TGTTGTGTCT GTGGAGGAGA AACGGCCAGG AGACGCAGCT AAACAAGTTT
    TACACTAAGA AGTGTCGGGA ATTGTACTAC TGTGTTAAAG ACAGCATGGA GCGTGCTGCT
    GCTCGACAAC AAAGCATCAA ACCAGGTCCA GAGCTTGGAG GCGAGTTTCC GGTTCAGGAC
    ATGAAGACAG GGGAGGGTGG TCTCCTCCAA GTCACACTGG AAGGCATCAA CCTCAAATTT
    ATGCACAGCC AGGTTTTCAT AGAGCTGAAT CACATTAAAA AGTGCAATAC AGTGAAAGGA
    GTCTTTGTCC TGGAGGAGTT TGTTCCTGAA ACTAAAGAAG TGGTGATCCA TAAGTACAAG
    ACTCCTATGG CACATCAGAT CTGTTACTCG GTGCTCTGCC TCTTCTCCTA CATGGCGGCG
    GTCAAGGGGA AAGAGTCAGA GGGCAAACCC AAGATGCTGT CCCCTCGTCC ACTAGCGAGC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48935
    Clone ID Related Accession (Same CDS sequence) XM_009303287.2
    Accession Version XM_009303287.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4860bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product MAP kinase-activating death domain protein isoform X24
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303287.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGTG TCTGGTAAGG
    TTGACTCTGA TCACCCAAAA GCAGATCTTC GCCTCCGATT CTAAGGCTCT GGCTAGCATG
    AGTCTAAACA CACAGCCTAT TCTGAACCTG GAGAAGTTTC AGGATGGACA GGAGTTGTCT
    TTATTGCCCC CCGGTCGTGA CAAAGCTTCC CCGTCCTCCA CTGAGTTCAA CCCACTCATC
    TACGGCAATG ATGTGGACTC AGTTGATGTT GCCACAAGGG TGGCAATGGT GAGGTTCTTC
    AACTCTCCAA ACGTGCTGCA AGGGTTCCAG ATGCACACAC GCACTCTCCG TCTCTTCCCT
    CGTCCTGTGG TGGCCTTTCA GTCCACATCT TTCCTGGCTT CTAGGCCACG ACGAAATGGA
    TTCACAGAAA AGCTCTCCCA CACTCAGGCA GTGGAGTACT ATGGAGAATG GGCTCTCAAC
    CCCACCAACC TGGCTTTCCA AAGGATTCAT AACAATGTGT ATGACCCCTC TCTAATAGGC
    GATAAGCCTA AGTGGTATGC CCACCAGTTA CAGCCAGTAT TCTACCGCGT GTATGATGGA
    AACTCTCATT TAGCGGAGGC CTTGAGTGGA CCCCTGCAGG ATGAGACCAA TGATTCAGAC
    CCAACAGACG ACAGTGGCAG CGACAGTGAA GCATATGATG ACTCCAGCTC CTCCTACTCA
    TCTCTTGGAG ATTTTGTGAA TGAGATGATC AAAGGGGACA TACAGGGAGA CACACCAAAT
    GTTGATCCCC TGACCCATGC TGCACTGGGA GATGCAGATG AGGTTGAGAT CCATGACTTT
    CACGAGTATA AGGGGAACAG CGGTGAACCA GAGCCAGAAG GCCCCACAGA AGCAGCGGAC
    AGTCAGCCCT TGCGGTCCAG CTCTAGCACC ACAGCCAGTT CCAGCCCCAG TACAGTCATA
    CAGGGAGTCA ACCATGAGCA GAAGGAACCT GTTGAGGTGG AGCCCATAGC AAATGTGACT
    TACCCAAGCT CTGCCCCAGT ATTGGGTCCT CCTCCTTTTA CAAGACCCAC CCCTGATCCA
    ACCCTTGTTG ACCCTGCCAA TAAGAAGCGA GAATATGACA ACCCCTATTT TGAGCCCCAG
    TACGGTTTCC CTACAGAGGA AGATGCTGAG ACTGATGAGC AGGAGGAGAG TTACACACCA
    CGATTTAACC AGAATCTCAA TGGCAATAAG CCTTCTCGTC CACTGAGACC CAGCAGCCTG
    AAGCTTCCGG GTGAATCTGA CGGAGAGGGA GACTCCAGAA ATAGCTCTCC TAACTCAACC
    ATCTCCAACA ATAGCAGTGA TGGCTTTGGG GGACTCATGT CCTTTGCCAG TAACTTGTAT
    AAAAACCATG GCACCAGTTT CAGTCTGTCC AGCCTGGCTC TGCCTAATAA AGCGAGAGAG
    AAGAACACGC CGTTTCCCAG CCTCAAAGGT GCCCGAGCCC CCAGAGCACT GGTAGATCAG
    AAATCCTCTG TTATTAAGCA CAGCCCTACT GTGAAGAGAG AGTCTCCGTC TCCTCAAGGC
    AGAGCCAACA ATACCAGTGA GAACCAGCAG TTTCTAAAGG AGGTGGTCCA GAGCGTGCTG
    GAGGGGCAGG GTGTGGGCTG GCTTAACATG AAGAAGGTGC GTCGCCTGCT AGAGAATGAA
    CAGCTCAGAG TCTTTGTGCT CAGCAAACTC AACCGTGCGG TCCAGTCAGA GGAGGACGCC
    CAGCAGGAAG TTATAAGAGA TGTGGAGATA AATAGAAAGG TCTACAAAGG CATGCTGGAT
    ATTTTGAAGT GCACGGTGTC CAGTTTGGAG CATTCATACA CTAATGCTGG CTTAGGGGGC
    ATGGCCAGTG TCTTCAGTCT GCTAGAGATA GCACGCACCC ACTACCAAAC CAAAGATCCA
    GAGAAGCGGA AACGGAGCCC CACCGAAGGG CTTAGCAGTC CAGGCAGTAA GGAGAGCCCA
    TCGGGCCGAA TGGAGAGTGC CAGGGCGGCA GGGGTGCTTC TGGTACCCCG TATCCAATTG
    CCGCCACCCT CCTCTGGGAA ATCATCACGC CAGTTTGATA CCCGGAGTCT GAACGAGGAG
    AATTTTATTG CCTCCATTGG AGCGGATGGA GTAAAACAAC GTGTAGAAGG AGGAGACACT
    GAGGAGAAGA AATCACAGAT CAGTGCAGAC AGTGGCCTCA GTGTGACATC TGGCTCACAG
    AAGAGTGATA CGGATTCCCT GGCGAGCTCT GAACCTCCTG CTCTGACCAG AAGCACCAGC
    CAGGACTCAG AGGCCAGCAC AGTAAGTAAC AGCTCAGGTG AGACGCTCGG TGCAGACAGT
    GACCTGAGCA GCACTGCTGG AGATGGCCTG ACAGGAAGAC ATGGTCAGCA TCTCAACCTG
    TCCCGTGGCA CACTGTCTGA CAGTGAGATT GAGACCAACC CTGCAACCAG CTCAGTGTTT
    GGCAAGACAC ACAAGCTGAA GCCAGGAGTG AAGGAGCCTG TTGGTGTTAA TAAAGCAGCA
    CCTGCTCCAC CCGTTGAGGA TGTTAGCATG AGGATCTATC TGTGTGAGGG GCTTCTGGGT
    AAAGAGCGCT CAACTCTGTG GGATCAGATG CAGTTCTGGG AGGACGCCTT CCTGGATGCC
    GTAATGCTGG AACGGGAAGG AATGGGAATG GATCAGGGAC CACAAGAAAT GATTGACAGG
    TATCTTTCTC TGGGAGATCA TGACCGCAAG AGACTGGAGG ATGATGAGGA CAGACTGCTG
    GCCACGCTTC TGCACAATAT GATTGCTTAC ATGCTTATGA TGAAGGTCGG TAAAAACGAC
    ATCAGGAAGA AAGTACGTCG TCTTATGGGC AAATCCCACA TTGGCCTCAC TCACAGTCAG
    GAGATCAATG AAGTCCTGGA TCGCCTCGCA CATTTAAGCG GTCGGGAGCT TTCTATCAGG
    CCGAGTGGCA GCAGGCATAT TAAAAAACAG ACATTTGTTG TGCATGCTGG GACAGATACA
    ACTGGTGACA TATTCTTCAT GGAGGTGTGT GATGATTGCA TCGTCTTGCG CAGTAATATT
    GGCACAGTCT ATGAGCGCTG GTGGTATGAG AAGCTCATAA ATATGACTTA CTGTCCCAAA
    ACTAAAGTGT TGTGTCTGTG GAGGAGAAAC GGCCAGGAGA CGCAGCTAAA CAAGTTTTAC
    ACTAAGAAGT GTCGGGAATT GTACTACTGT GTTAAAGACA GCATGGAGCG TGCTGCTGCT
    CGACAACAAA GCATCAAACC AGGTCCAGAG CTTGGAGGCG AGTTTCCGGT TCAGGACATG
    AAGACAGGGG AGGGTGGTCT CCTCCAAGTC ACACTGGAAG GCATCAACCT CAAATTTATG
    CACAGCCAGG TTTTCATAGA GCTGAATCAC ATTAAAAAGT GCAATACAGT GAAAGGAGTC
    TTTGTCCTGG AGGAGTTTGT TCCTGAAACT AAAGAAGTGG TGATCCATAA GTACAAGACT
    CCTATGGCAC ATCAGATCTG TTACTCGGTG CTCTGCCTCT TCTCCTACAT GGCGGCGGTC
    AAGGGGAAAG AGTCAGAGGG CAAACCCAAG ATGCTGTCCC CTCGTCCACT AGCGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301562.1
    CDS435..5294
    Translation

    Target ORF information:

    RefSeq Version XM_009303287.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303287.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGTG TCTGGTAAGG
    TTGACTCTGA TCACCCAAAA GCAGATCTTC GCCTCCGATT CTAAGGCTCT GGCTAGCATG
    AGTCTAAACA CACAGCCTAT TCTGAACCTG GAGAAGTTTC AGGATGGACA GGAGTTGTCT
    TTATTGCCCC CCGGTCGTGA CAAAGCTTCC CCGTCCTCCA CTGAGTTCAA CCCACTCATC
    TACGGCAATG ATGTGGACTC AGTTGATGTT GCCACAAGGG TGGCAATGGT GAGGTTCTTC
    AACTCTCCAA ACGTGCTGCA AGGGTTCCAG ATGCACACAC GCACTCTCCG TCTCTTCCCT
    CGTCCTGTGG TGGCCTTTCA GTCCACATCT TTCCTGGCTT CTAGGCCACG ACGAAATGGA
    TTCACAGAAA AGCTCTCCCA CACTCAGGCA GTGGAGTACT ATGGAGAATG GGCTCTCAAC
    CCCACCAACC TGGCTTTCCA AAGGATTCAT AACAATGTGT ATGACCCCTC TCTAATAGGC
    GATAAGCCTA AGTGGTATGC CCACCAGTTA CAGCCAGTAT TCTACCGCGT GTATGATGGA
    AACTCTCATT TAGCGGAGGC CTTGAGTGGA CCCCTGCAGG ATGAGACCAA TGATTCAGAC
    CCAACAGACG ACAGTGGCAG CGACAGTGAA GCATATGATG ACTCCAGCTC CTCCTACTCA
    TCTCTTGGAG ATTTTGTGAA TGAGATGATC AAAGGGGACA TACAGGGAGA CACACCAAAT
    GTTGATCCCC TGACCCATGC TGCACTGGGA GATGCAGATG AGGTTGAGAT CCATGACTTT
    CACGAGTATA AGGGGAACAG CGGTGAACCA GAGCCAGAAG GCCCCACAGA AGCAGCGGAC
    AGTCAGCCCT TGCGGTCCAG CTCTAGCACC ACAGCCAGTT CCAGCCCCAG TACAGTCATA
    CAGGGAGTCA ACCATGAGCA GAAGGAACCT GTTGAGGTGG AGCCCATAGC AAATGTGACT
    TACCCAAGCT CTGCCCCAGT ATTGGGTCCT CCTCCTTTTA CAAGACCCAC CCCTGATCCA
    ACCCTTGTTG ACCCTGCCAA TAAGAAGCGA GAATATGACA ACCCCTATTT TGAGCCCCAG
    TACGGTTTCC CTACAGAGGA AGATGCTGAG ACTGATGAGC AGGAGGAGAG TTACACACCA
    CGATTTAACC AGAATCTCAA TGGCAATAAG CCTTCTCGTC CACTGAGACC CAGCAGCCTG
    AAGCTTCCGG GTGAATCTGA CGGAGAGGGA GACTCCAGAA ATAGCTCTCC TAACTCAACC
    ATCTCCAACA ATAGCAGTGA TGGCTTTGGG GGACTCATGT CCTTTGCCAG TAACTTGTAT
    AAAAACCATG GCACCAGTTT CAGTCTGTCC AGCCTGGCTC TGCCTAATAA AGCGAGAGAG
    AAGAACACGC CGTTTCCCAG CCTCAAAGGT GCCCGAGCCC CCAGAGCACT GGTAGATCAG
    AAATCCTCTG TTATTAAGCA CAGCCCTACT GTGAAGAGAG AGTCTCCGTC TCCTCAAGGC
    AGAGCCAACA ATACCAGTGA GAACCAGCAG TTTCTAAAGG AGGTGGTCCA GAGCGTGCTG
    GAGGGGCAGG GTGTGGGCTG GCTTAACATG AAGAAGGTGC GTCGCCTGCT AGAGAATGAA
    CAGCTCAGAG TCTTTGTGCT CAGCAAACTC AACCGTGCGG TCCAGTCAGA GGAGGACGCC
    CAGCAGGAAG TTATAAGAGA TGTGGAGATA AATAGAAAGG TCTACAAAGG CATGCTGGAT
    ATTTTGAAGT GCACGGTGTC CAGTTTGGAG CATTCATACA CTAATGCTGG CTTAGGGGGC
    ATGGCCAGTG TCTTCAGTCT GCTAGAGATA GCACGCACCC ACTACCAAAC CAAAGATCCA
    GAGAAGCGGA AACGGAGCCC CACCGAAGGG CTTAGCAGTC CAGGCAGTAA GGAGAGCCCA
    TCGGGCCGAA TGGAGAGTGC CAGGGCGGCA GGGGTGCTTC TGGTACCCCG TATCCAATTG
    CCGCCACCCT CCTCTGGGAA ATCATCACGC CAGTTTGATA CCCGGAGTCT GAACGAGGAG
    AATTTTATTG CCTCCATTGG AGCGGATGGA GTAAAACAAC GTGTAGAAGG AGGAGACACT
    GAGGAGAAGA AATCACAGAT CAGTGCAGAC AGTGGCCTCA GTGTGACATC TGGCTCACAG
    AAGAGTGATA CGGATTCCCT GGCGAGCTCT GAACCTCCTG CTCTGACCAG AAGCACCAGC
    CAGGACTCAG AGGCCAGCAC AGTAAGTAAC AGCTCAGGTG AGACGCTCGG TGCAGACAGT
    GACCTGAGCA GCACTGCTGG AGATGGCCTG ACAGGAAGAC ATGGTCAGCA TCTCAACCTG
    TCCCGTGGCA CACTGTCTGA CAGTGAGATT GAGACCAACC CTGCAACCAG CTCAGTGTTT
    GGCAAGACAC ACAAGCTGAA GCCAGGAGTG AAGGAGCCTG TTGGTGTTAA TAAAGCAGCA
    CCTGCTCCAC CCGTTGAGGA TGTTAGCATG AGGATCTATC TGTGTGAGGG GCTTCTGGGT
    AAAGAGCGCT CAACTCTGTG GGATCAGATG CAGTTCTGGG AGGACGCCTT CCTGGATGCC
    GTAATGCTGG AACGGGAAGG AATGGGAATG GATCAGGGAC CACAAGAAAT GATTGACAGG
    TATCTTTCTC TGGGAGATCA TGACCGCAAG AGACTGGAGG ATGATGAGGA CAGACTGCTG
    GCCACGCTTC TGCACAATAT GATTGCTTAC ATGCTTATGA TGAAGGTCGG TAAAAACGAC
    ATCAGGAAGA AAGTACGTCG TCTTATGGGC AAATCCCACA TTGGCCTCAC TCACAGTCAG
    GAGATCAATG AAGTCCTGGA TCGCCTCGCA CATTTAAGCG GTCGGGAGCT TTCTATCAGG
    CCGAGTGGCA GCAGGCATAT TAAAAAACAG ACATTTGTTG TGCATGCTGG GACAGATACA
    ACTGGTGACA TATTCTTCAT GGAGGTGTGT GATGATTGCA TCGTCTTGCG CAGTAATATT
    GGCACAGTCT ATGAGCGCTG GTGGTATGAG AAGCTCATAA ATATGACTTA CTGTCCCAAA
    ACTAAAGTGT TGTGTCTGTG GAGGAGAAAC GGCCAGGAGA CGCAGCTAAA CAAGTTTTAC
    ACTAAGAAGT GTCGGGAATT GTACTACTGT GTTAAAGACA GCATGGAGCG TGCTGCTGCT
    CGACAACAAA GCATCAAACC AGGTCCAGAG CTTGGAGGCG AGTTTCCGGT TCAGGACATG
    AAGACAGGGG AGGGTGGTCT CCTCCAAGTC ACACTGGAAG GCATCAACCT CAAATTTATG
    CACAGCCAGG TTTTCATAGA GCTGAATCAC ATTAAAAAGT GCAATACAGT GAAAGGAGTC
    TTTGTCCTGG AGGAGTTTGT TCCTGAAACT AAAGAAGTGG TGATCCATAA GTACAAGACT
    CCTATGGCAC ATCAGATCTG TTACTCGGTG CTCTGCCTCT TCTCCTACAT GGCGGCGGTC
    AAGGGGAAAG AGTCAGAGGG CAAACCCAAG ATGCTGTCCC CTCGTCCACT AGCGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48936
    Clone ID Related Accession (Same CDS sequence) XM_009303288.2
    Accession Version XM_009303288.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4821bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product MAP kinase-activating death domain protein isoform X25
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303288.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGTG TCTGGTAAGG
    TTGACTCTGA TCACCCAAAA GCAGATCTTC GCCTCCGATT CTAAGGCTCT GGCTAGCATG
    AGTCTAAACA CACAGCCTAT TCTGAACCTG GAGAAGTTTC AGGATGGACA GGAGTTGTCT
    TTATTGCCCC CCGGTCGTGA CAAAGCTTCC CCGTCCTCCA CTGAGTTCAA CCCACTCATC
    TACGGCAATG ATGTGGACTC AGTTGATGTT GCCACAAGGG TGGCAATGGT GAGGTTCTTC
    AACTCTCCAA ACGTGCTGCA AGGGTTCCAG ATGCACACAC GCACTCTCCG TCTCTTCCCT
    CGTCCTGTGG TGGCCTTTCA GTCCACATCT TTCCTGGCTT CTAGGCCACG ACGAAATGGA
    TTCACAGAAA AGCTCTCCCA CACTCAGGCA GTGGAGTACT ATGGAGAATG GGCTCTCAAC
    CCCACCAACC TGGCTTTCCA AAGGATTCAT AACAATGTGT ATGACCCCTC TCTAATAGGC
    GATAAGCCTA AGTGGTATGC CCACCAGTTA CAGCCAGTAT TCTACCGCGT GTATGATGGA
    AACTCTCATT TAGCGGAGGC CTTGAGTGGA CCCCTGCAGG ATGAGACCAA TGATTCAGAC
    CCAACAGACG ACAGTGGCAG CGACAGTGAA GCATATGATG ACTCCAGCTC CTCCTACTCA
    TCTCTTGGAG ATTTTGTGAA TGAGATGATC AAAGGGGACA TACAGGGAGA CACACCAAAT
    GTTGATCCCC TGACCCATGC TGCACTGGGA GATGCAGATG AGGTTGAGAT CCATGACTTT
    CACGAGTATA AGGGGAACAG CGGTGAACCA GAGCCAGAAG GCCCCACAGA AGCAGCGGAC
    AGTCAGCCCT TGCGGTCCAG CTCTAGCACC ACAGCCAGTT CCAGCCCCAG TACAGTCATA
    CAGGGAGTCA ACCATGAGCA GAAGGAACCT GTTGAGGTGG AGCCCATAGC AAATGTGACT
    TACCCAAGCT CTGCCCCAGT ATTGGGTCCT CCTCCTTTTA CAAGACCCAC CCCTGATCCA
    ACCCTTGTTG ACCCTGCCAA TAAGAAGCGA GAATATGACA ACCCCTATTT TGAGCCCCAG
    TACGGTTTCC CTACAGAGGA AGATGCTGAG ACTGATGAGC AGGAGGAGAG TTACACACCA
    CGATTTAACC AGAATCTCAA TGGCAATAAG CCTTCTCGTC CACTGAGACC CAGCAGCCTG
    AAGCTTCCGG GTGAATCTGA CGGAGAGGGA GACTCCAGAA ATAGCTCTCC TAACTCAACC
    ATCTCCAACA ATAGCAGTGA TGGCTTTGGG GGACTCATGT CCTTTGCCAG TAACTTGTAT
    AAAAACCATG GCACCAGTTT CAGTCTGTCC AGCCTGGCTC TGCCTAATAA AGCGAGAGAG
    AAGAACACGC CGTTTCCCAG CCTCAAAGAC GATGCTGATG ACAGTCCTGA AAGTCCTGTA
    TTTGGGCTAA ATTCTCTAAT GGAGATTGTA ACAGAGATCG GCCCGGGGAG CGGAGAAGGT
    GCCCGAGCCC CCAGAGCACT GGTAGATCAG AAATCCTCTG TTATTAAGCA CAGCCCTACT
    GTGAAGAGAG AGTCTCCGTC TCCTCAAGGC AGAGCCAACA ATACCAGTGA GAACCAGCAG
    TTTCTAAAGG AGGTGGTCCA GAGCGTGCTG GAGGGGCAGG GTGTGGGCTG GCTTAACATG
    AAGAAGGTGC GTCGCCTGCT AGAGAATGAA CAGCTCAGAG TCTTTGTGCT CAGCAAACTC
    AACCGTGCGG TCCAGTCAGA GGAGGACGCC CAGCAGGAAG TTATAAGAGA TGTGGAGATA
    AATAGAAAGG TCTACAAAGG CATGCTGGAT ATTTTGAAGT GCACGGTGTC CAGTTTGGAG
    CATTCATACA CTAATGCTGG CTTAGGGGGC ATGGCCAGTG TCTTCAGTCT GCTAGAGATA
    GCACGCACCC ACTACCAAAC CAAAGGAGCG GATGGAGTAA AACAACGTGT AGAAGGAGGA
    GACACTGAGG AGAAGAAATC ACAGATCAGT GCAGACAGTG GCCTCAGTGT GACATCTGGC
    TCACAGAAGA GTGATACGGA TTCCCTGGCG AGCTCTGAAC CTCCTGCTCT GACCAGAAGC
    ACCAGCCAGG ACTCAGAGGC CAGCACAGTG GTAAGTAACA GCTCAGGTGA GACGCTCGGT
    GCAGACAGTG ACCTGAGCAG CACTGCTGGA GATGGCCTGA CAGGAAGACA TGGTCAGCAT
    CTCAACCTGT CCCGTGGCAC ACTGTCTGAC AGTGAGATTG AGACCAACCC TGCAACCAGC
    TCAGTGTTTG GCAAGACACA CAAGCTGAAG CCAGGAGTGA AGGAGCCTGT TGGTGTTAAT
    AAAGCAGCAC CTGCTCCACC CGTTGAGGAT GTTAGCATGA GGATCTATCT GTGTGAGGGG
    CTTCTGGGCC GGGATAAAAG CTCAGTTTGG GACCAGTTGG AGGATGCTGC AATGGAAACC
    TTTTCTCTGA GTAAAGAGCG CTCAACTCTG TGGGATCAGA TGCAGTTCTG GGAGGACGCC
    TTCCTGGATG CCGTAATGCT GGAACGGGAA GGAATGGGAA TGGATCAGGG ACCACAAGAA
    ATGATTGACA GGTATCTTTC TCTGGGAGAT CATGACCGCA AGAGACTGGA GGATGATGAG
    GACAGACTGC TGGCCACGCT TCTGCACAAT ATGATTGCTT ACATGCTTAT GATGAAGGTC
    GGTAAAAACG ACATCAGGAA GAAAGTACGT CGTCTTATGG GCAAATCCCA CATTGGCCTC
    ACTCACAGTC AGGAGATCAA TGAAGTCCTG GATCGCCTCG CACATTTAAG CGGTCGGGAG
    CTTTCTATCA GGCCGAGTGG CAGCAGGCAT ATTAAAAAAC AGACATTTGT TGTGCATGCT
    GGGACAGATA CAACTGGTGA CATATTCTTC ATGGAGGTGT GTGATGATTG CATCGTCTTG
    CGCAGTAATA TTGGCACAGT CTATGAGCGC TGGTGGTATG AGAAGCTCAT AAATATGACT
    TACTGTCCCA AAACTAAAGT GTTGTGTCTG TGGAGGAGAA ACGGCCAGGA GACGCAGCTA
    AACAAGTTTT ACACTAAGAA GTGTCGGGAA TTGTACTACT GTGTTAAAGA CAGCATGGAG
    CGTGCTGCTG CTCGACAACA AAGCATCAAA CCAGGTCCAG AGCTTGGAGG CGAGTTTCCG
    GTTCAGGACA TGAAGACAGG GGAGGGTGGT CTCCTCCAAG TCACACTGGA AGGCATCAAC
    CTCAAATTTA TGCACAGCCA GGAGCGGAAG GTTTTCATAG AGCTGAATCA CATTAAAAAG
    TGCAATACAG TGAAAGGAGT CTTTGTCCTG GAGGAGTTTG TTCCTGAAAC TAAAGAAGTG
    GTGATCCATA AGTACAAGAC TCCTATGGCA CATCAGATCT GTTACTCGGT GCTCTGCCTC
    TTCTCCTACA TGGCGGCGGT CAAGGGGAAA GAGTCAGAGG GCAAACCCAA GATGCTGTCC
    CCTCGTCCAC TAGCGAGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301563.1
    CDS435..5255
    Translation

    Target ORF information:

    RefSeq Version XM_009303288.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X25, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303288.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGTG TCTGGTAAGG
    TTGACTCTGA TCACCCAAAA GCAGATCTTC GCCTCCGATT CTAAGGCTCT GGCTAGCATG
    AGTCTAAACA CACAGCCTAT TCTGAACCTG GAGAAGTTTC AGGATGGACA GGAGTTGTCT
    TTATTGCCCC CCGGTCGTGA CAAAGCTTCC CCGTCCTCCA CTGAGTTCAA CCCACTCATC
    TACGGCAATG ATGTGGACTC AGTTGATGTT GCCACAAGGG TGGCAATGGT GAGGTTCTTC
    AACTCTCCAA ACGTGCTGCA AGGGTTCCAG ATGCACACAC GCACTCTCCG TCTCTTCCCT
    CGTCCTGTGG TGGCCTTTCA GTCCACATCT TTCCTGGCTT CTAGGCCACG ACGAAATGGA
    TTCACAGAAA AGCTCTCCCA CACTCAGGCA GTGGAGTACT ATGGAGAATG GGCTCTCAAC
    CCCACCAACC TGGCTTTCCA AAGGATTCAT AACAATGTGT ATGACCCCTC TCTAATAGGC
    GATAAGCCTA AGTGGTATGC CCACCAGTTA CAGCCAGTAT TCTACCGCGT GTATGATGGA
    AACTCTCATT TAGCGGAGGC CTTGAGTGGA CCCCTGCAGG ATGAGACCAA TGATTCAGAC
    CCAACAGACG ACAGTGGCAG CGACAGTGAA GCATATGATG ACTCCAGCTC CTCCTACTCA
    TCTCTTGGAG ATTTTGTGAA TGAGATGATC AAAGGGGACA TACAGGGAGA CACACCAAAT
    GTTGATCCCC TGACCCATGC TGCACTGGGA GATGCAGATG AGGTTGAGAT CCATGACTTT
    CACGAGTATA AGGGGAACAG CGGTGAACCA GAGCCAGAAG GCCCCACAGA AGCAGCGGAC
    AGTCAGCCCT TGCGGTCCAG CTCTAGCACC ACAGCCAGTT CCAGCCCCAG TACAGTCATA
    CAGGGAGTCA ACCATGAGCA GAAGGAACCT GTTGAGGTGG AGCCCATAGC AAATGTGACT
    TACCCAAGCT CTGCCCCAGT ATTGGGTCCT CCTCCTTTTA CAAGACCCAC CCCTGATCCA
    ACCCTTGTTG ACCCTGCCAA TAAGAAGCGA GAATATGACA ACCCCTATTT TGAGCCCCAG
    TACGGTTTCC CTACAGAGGA AGATGCTGAG ACTGATGAGC AGGAGGAGAG TTACACACCA
    CGATTTAACC AGAATCTCAA TGGCAATAAG CCTTCTCGTC CACTGAGACC CAGCAGCCTG
    AAGCTTCCGG GTGAATCTGA CGGAGAGGGA GACTCCAGAA ATAGCTCTCC TAACTCAACC
    ATCTCCAACA ATAGCAGTGA TGGCTTTGGG GGACTCATGT CCTTTGCCAG TAACTTGTAT
    AAAAACCATG GCACCAGTTT CAGTCTGTCC AGCCTGGCTC TGCCTAATAA AGCGAGAGAG
    AAGAACACGC CGTTTCCCAG CCTCAAAGAC GATGCTGATG ACAGTCCTGA AAGTCCTGTA
    TTTGGGCTAA ATTCTCTAAT GGAGATTGTA ACAGAGATCG GCCCGGGGAG CGGAGAAGGT
    GCCCGAGCCC CCAGAGCACT GGTAGATCAG AAATCCTCTG TTATTAAGCA CAGCCCTACT
    GTGAAGAGAG AGTCTCCGTC TCCTCAAGGC AGAGCCAACA ATACCAGTGA GAACCAGCAG
    TTTCTAAAGG AGGTGGTCCA GAGCGTGCTG GAGGGGCAGG GTGTGGGCTG GCTTAACATG
    AAGAAGGTGC GTCGCCTGCT AGAGAATGAA CAGCTCAGAG TCTTTGTGCT CAGCAAACTC
    AACCGTGCGG TCCAGTCAGA GGAGGACGCC CAGCAGGAAG TTATAAGAGA TGTGGAGATA
    AATAGAAAGG TCTACAAAGG CATGCTGGAT ATTTTGAAGT GCACGGTGTC CAGTTTGGAG
    CATTCATACA CTAATGCTGG CTTAGGGGGC ATGGCCAGTG TCTTCAGTCT GCTAGAGATA
    GCACGCACCC ACTACCAAAC CAAAGGAGCG GATGGAGTAA AACAACGTGT AGAAGGAGGA
    GACACTGAGG AGAAGAAATC ACAGATCAGT GCAGACAGTG GCCTCAGTGT GACATCTGGC
    TCACAGAAGA GTGATACGGA TTCCCTGGCG AGCTCTGAAC CTCCTGCTCT GACCAGAAGC
    ACCAGCCAGG ACTCAGAGGC CAGCACAGTG GTAAGTAACA GCTCAGGTGA GACGCTCGGT
    GCAGACAGTG ACCTGAGCAG CACTGCTGGA GATGGCCTGA CAGGAAGACA TGGTCAGCAT
    CTCAACCTGT CCCGTGGCAC ACTGTCTGAC AGTGAGATTG AGACCAACCC TGCAACCAGC
    TCAGTGTTTG GCAAGACACA CAAGCTGAAG CCAGGAGTGA AGGAGCCTGT TGGTGTTAAT
    AAAGCAGCAC CTGCTCCACC CGTTGAGGAT GTTAGCATGA GGATCTATCT GTGTGAGGGG
    CTTCTGGGCC GGGATAAAAG CTCAGTTTGG GACCAGTTGG AGGATGCTGC AATGGAAACC
    TTTTCTCTGA GTAAAGAGCG CTCAACTCTG TGGGATCAGA TGCAGTTCTG GGAGGACGCC
    TTCCTGGATG CCGTAATGCT GGAACGGGAA GGAATGGGAA TGGATCAGGG ACCACAAGAA
    ATGATTGACA GGTATCTTTC TCTGGGAGAT CATGACCGCA AGAGACTGGA GGATGATGAG
    GACAGACTGC TGGCCACGCT TCTGCACAAT ATGATTGCTT ACATGCTTAT GATGAAGGTC
    GGTAAAAACG ACATCAGGAA GAAAGTACGT CGTCTTATGG GCAAATCCCA CATTGGCCTC
    ACTCACAGTC AGGAGATCAA TGAAGTCCTG GATCGCCTCG CACATTTAAG CGGTCGGGAG
    CTTTCTATCA GGCCGAGTGG CAGCAGGCAT ATTAAAAAAC AGACATTTGT TGTGCATGCT
    GGGACAGATA CAACTGGTGA CATATTCTTC ATGGAGGTGT GTGATGATTG CATCGTCTTG
    CGCAGTAATA TTGGCACAGT CTATGAGCGC TGGTGGTATG AGAAGCTCAT AAATATGACT
    TACTGTCCCA AAACTAAAGT GTTGTGTCTG TGGAGGAGAA ACGGCCAGGA GACGCAGCTA
    AACAAGTTTT ACACTAAGAA GTGTCGGGAA TTGTACTACT GTGTTAAAGA CAGCATGGAG
    CGTGCTGCTG CTCGACAACA AAGCATCAAA CCAGGTCCAG AGCTTGGAGG CGAGTTTCCG
    GTTCAGGACA TGAAGACAGG GGAGGGTGGT CTCCTCCAAG TCACACTGGA AGGCATCAAC
    CTCAAATTTA TGCACAGCCA GGAGCGGAAG GTTTTCATAG AGCTGAATCA CATTAAAAAG
    TGCAATACAG TGAAAGGAGT CTTTGTCCTG GAGGAGTTTG TTCCTGAAAC TAAAGAAGTG
    GTGATCCATA AGTACAAGAC TCCTATGGCA CATCAGATCT GTTACTCGGT GCTCTGCCTC
    TTCTCCTACA TGGCGGCGGT CAAGGGGAAA GAGTCAGAGG GCAAACCCAA GATGCTGTCC
    CCTCGTCCAC TAGCGAGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48937
    Clone ID Related Accession (Same CDS sequence) XM_009303289.2 , XM_021477903.1
    Accession Version XM_009303289.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4815bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product MAP kinase-activating death domain protein isoform X26
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303289.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGGAGCGGAT GGAGTAAAAC AACGTGTAGA AGGAGGAGAC
    ACTGAGGAGA AGAAATCACA GATCAGTGCA GACAGTGGCC TCAGTGTGAC ATCTGGCTCA
    CAGAAGAGTG ATACGGATTC CCTGGCGAGC TCTGAACCTC CTGCTCTGAC CAGAAGCACC
    AGCCAGGACT CAGAGGCCAG CACAGTGGTA AGTAACAGCT CAGGTGAGAC GCTCGGTGCA
    GACAGTGACC TGAGCAGCAC TGCTGGAGAT GGCCTGACAG GAAGACATGG TCAGCATCTC
    AACCTGTCCC GTGGCACACT GTCTGACAGT GAGATTGAGA CCAACCCTGC AACCAGCTCA
    GTGTTTGGCA AGACACACAA GCTGAAGCCA GGAGTGAAGG AGCCTGTTGG TGTTAATAAA
    GCAGCACCTG CTCCACCCGT TGAGGATGTT AGCATGAGGA TCTATCTGTG TGAGGGGCTT
    CTGGGTAAAG AGCGCTCAAC TCTGTGGGAT CAGATGCAGT TCTGGGAGGA CGCCTTCCTG
    GATGCCGTAA TGCTGGAACG GGAAGGAATG GGAATGGATC AGGGACCACA AGAAATGATT
    GACAGGTATC TTTCTCTGGG AGATCATGAC CGCAAGAGAC TGGAGGATGA TGAGGACAGA
    CTGCTGGCCA CGCTTCTGCA CAATATGATT GCTTACATGC TTATGATGAA GGTCGGTAAA
    AACGACATCA GGAAGAAAGT ACGTCGTCTT ATGGGCAAAT CCCACATTGG CCTCACTCAC
    AGTCAGGAGA TCAATGAAGT CCTGGATCGC CTCGCACATT TAAGCGGTCG GGAGCTTTCT
    ATCAGGCCGA GTGGCAGCAG GCATATTAAA AAACAGACAT TTGTTGTGCA TGCTGGGACA
    GATACAACTG GTGACATATT CTTCATGGAG GTGTGTGATG ATTGCATCGT CTTGCGCAGT
    AATATTGGCA CAGTCTATGA GCGCTGGTGG TATGAGAAGC TCATAAATAT GACTTACTGT
    CCCAAAACTA AAGTGTTGTG TCTGTGGAGG AGAAACGGCC AGGAGACGCA GCTAAACAAG
    TTTTACACTA AGAAGTGTCG GGAATTGTAC TACTGTGTTA AAGACAGCAT GGAGCGTGCT
    GCTGCTCGAC AACAAAGCAT CAAACCAGGT CCAGAGCTTG GAGGCGAGTT TCCGGTTCAG
    GACATGAAGA CAGGGGAGGG TGGTCTCCTC CAAGTCACAC TGGAAGGCAT CAACCTCAAA
    TTTATGCACA GCCAGGAGCG GAAGGTTTTC ATAGAGCTGA ATCACATTAA AAAGTGCAAT
    ACAGTGAAAG GAGTCTTTGT CCTGGAGGAG TTTGTTCCTG AAACTAAAGA AGTGGTGATC
    CATAAGTACA AGACTCCTAT GGCACATCAG ATCTGTTACT CGGTGCTCTG CCTCTTCTCC
    TACATGGCGG CGGTCAAGGG GAAAGAGTCA GAGGGCAAAC CCAAGATGCT GTCCCCTCGT
    CCACTAGCGA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301564.1
    CDS435..5249
    Translation

    Target ORF information:

    RefSeq Version XM_009303289.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X26, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303289.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGGAGCGGAT GGAGTAAAAC AACGTGTAGA AGGAGGAGAC
    ACTGAGGAGA AGAAATCACA GATCAGTGCA GACAGTGGCC TCAGTGTGAC ATCTGGCTCA
    CAGAAGAGTG ATACGGATTC CCTGGCGAGC TCTGAACCTC CTGCTCTGAC CAGAAGCACC
    AGCCAGGACT CAGAGGCCAG CACAGTGGTA AGTAACAGCT CAGGTGAGAC GCTCGGTGCA
    GACAGTGACC TGAGCAGCAC TGCTGGAGAT GGCCTGACAG GAAGACATGG TCAGCATCTC
    AACCTGTCCC GTGGCACACT GTCTGACAGT GAGATTGAGA CCAACCCTGC AACCAGCTCA
    GTGTTTGGCA AGACACACAA GCTGAAGCCA GGAGTGAAGG AGCCTGTTGG TGTTAATAAA
    GCAGCACCTG CTCCACCCGT TGAGGATGTT AGCATGAGGA TCTATCTGTG TGAGGGGCTT
    CTGGGTAAAG AGCGCTCAAC TCTGTGGGAT CAGATGCAGT TCTGGGAGGA CGCCTTCCTG
    GATGCCGTAA TGCTGGAACG GGAAGGAATG GGAATGGATC AGGGACCACA AGAAATGATT
    GACAGGTATC TTTCTCTGGG AGATCATGAC CGCAAGAGAC TGGAGGATGA TGAGGACAGA
    CTGCTGGCCA CGCTTCTGCA CAATATGATT GCTTACATGC TTATGATGAA GGTCGGTAAA
    AACGACATCA GGAAGAAAGT ACGTCGTCTT ATGGGCAAAT CCCACATTGG CCTCACTCAC
    AGTCAGGAGA TCAATGAAGT CCTGGATCGC CTCGCACATT TAAGCGGTCG GGAGCTTTCT
    ATCAGGCCGA GTGGCAGCAG GCATATTAAA AAACAGACAT TTGTTGTGCA TGCTGGGACA
    GATACAACTG GTGACATATT CTTCATGGAG GTGTGTGATG ATTGCATCGT CTTGCGCAGT
    AATATTGGCA CAGTCTATGA GCGCTGGTGG TATGAGAAGC TCATAAATAT GACTTACTGT
    CCCAAAACTA AAGTGTTGTG TCTGTGGAGG AGAAACGGCC AGGAGACGCA GCTAAACAAG
    TTTTACACTA AGAAGTGTCG GGAATTGTAC TACTGTGTTA AAGACAGCAT GGAGCGTGCT
    GCTGCTCGAC AACAAAGCAT CAAACCAGGT CCAGAGCTTG GAGGCGAGTT TCCGGTTCAG
    GACATGAAGA CAGGGGAGGG TGGTCTCCTC CAAGTCACAC TGGAAGGCAT CAACCTCAAA
    TTTATGCACA GCCAGGAGCG GAAGGTTTTC ATAGAGCTGA ATCACATTAA AAAGTGCAAT
    ACAGTGAAAG GAGTCTTTGT CCTGGAGGAG TTTGTTCCTG AAACTAAAGA AGTGGTGATC
    CATAAGTACA AGACTCCTAT GGCACATCAG ATCTGTTACT CGGTGCTCTG CCTCTTCTCC
    TACATGGCGG CGGTCAAGGG GAAAGAGTCA GAGGGCAAAC CCAAGATGCT GTCCCCTCGT
    CCACTAGCGA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48937
    Clone ID Related Accession (Same CDS sequence) XM_009303289.2 , XM_021477903.1
    Accession Version XM_021477903.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4815bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X29
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGGAGCGGAT GGAGTAAAAC AACGTGTAGA AGGAGGAGAC
    ACTGAGGAGA AGAAATCACA GATCAGTGCA GACAGTGGCC TCAGTGTGAC ATCTGGCTCA
    CAGAAGAGTG ATACGGATTC CCTGGCGAGC TCTGAACCTC CTGCTCTGAC CAGAAGCACC
    AGCCAGGACT CAGAGGCCAG CACAGTGGTA AGTAACAGCT CAGGTGAGAC GCTCGGTGCA
    GACAGTGACC TGAGCAGCAC TGCTGGAGAT GGCCTGACAG GAAGACATGG TCAGCATCTC
    AACCTGTCCC GTGGCACACT GTCTGACAGT GAGATTGAGA CCAACCCTGC AACCAGCTCA
    GTGTTTGGCA AGACACACAA GCTGAAGCCA GGAGTGAAGG AGCCTGTTGG TGTTAATAAA
    GCAGCACCTG CTCCACCCGT TGAGGATGTT AGCATGAGGA TCTATCTGTG TGAGGGGCTT
    CTGGGTAAAG AGCGCTCAAC TCTGTGGGAT CAGATGCAGT TCTGGGAGGA CGCCTTCCTG
    GATGCCGTAA TGCTGGAACG GGAAGGAATG GGAATGGATC AGGGACCACA AGAAATGATT
    GACAGGTATC TTTCTCTGGG AGATCATGAC CGCAAGAGAC TGGAGGATGA TGAGGACAGA
    CTGCTGGCCA CGCTTCTGCA CAATATGATT GCTTACATGC TTATGATGAA GGTCGGTAAA
    AACGACATCA GGAAGAAAGT ACGTCGTCTT ATGGGCAAAT CCCACATTGG CCTCACTCAC
    AGTCAGGAGA TCAATGAAGT CCTGGATCGC CTCGCACATT TAAGCGGTCG GGAGCTTTCT
    ATCAGGCCGA GTGGCAGCAG GCATATTAAA AAACAGACAT TTGTTGTGCA TGCTGGGACA
    GATACAACTG GTGACATATT CTTCATGGAG GTGTGTGATG ATTGCATCGT CTTGCGCAGT
    AATATTGGCA CAGTCTATGA GCGCTGGTGG TATGAGAAGC TCATAAATAT GACTTACTGT
    CCCAAAACTA AAGTGTTGTG TCTGTGGAGG AGAAACGGCC AGGAGACGCA GCTAAACAAG
    TTTTACACTA AGAAGTGTCG GGAATTGTAC TACTGTGTTA AAGACAGCAT GGAGCGTGCT
    GCTGCTCGAC AACAAAGCAT CAAACCAGGT CCAGAGCTTG GAGGCGAGTT TCCGGTTCAG
    GACATGAAGA CAGGGGAGGG TGGTCTCCTC CAAGTCACAC TGGAAGGCAT CAACCTCAAA
    TTTATGCACA GCCAGGAGCG GAAGGTTTTC ATAGAGCTGA ATCACATTAA AAAGTGCAAT
    ACAGTGAAAG GAGTCTTTGT CCTGGAGGAG TTTGTTCCTG AAACTAAAGA AGTGGTGATC
    CATAAGTACA AGACTCCTAT GGCACATCAG ATCTGTTACT CGGTGCTCTG CCTCTTCTCC
    TACATGGCGG CGGTCAAGGG GAAAGAGTCA GAGGGCAAAC CCAAGATGCT GTCCCCTCGT
    CCACTAGCGA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333578.1
    CDS1..4815
    Translation

    Target ORF information:

    RefSeq Version XM_021477903.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021477903.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGGAGCGGAT GGAGTAAAAC AACGTGTAGA AGGAGGAGAC
    ACTGAGGAGA AGAAATCACA GATCAGTGCA GACAGTGGCC TCAGTGTGAC ATCTGGCTCA
    CAGAAGAGTG ATACGGATTC CCTGGCGAGC TCTGAACCTC CTGCTCTGAC CAGAAGCACC
    AGCCAGGACT CAGAGGCCAG CACAGTGGTA AGTAACAGCT CAGGTGAGAC GCTCGGTGCA
    GACAGTGACC TGAGCAGCAC TGCTGGAGAT GGCCTGACAG GAAGACATGG TCAGCATCTC
    AACCTGTCCC GTGGCACACT GTCTGACAGT GAGATTGAGA CCAACCCTGC AACCAGCTCA
    GTGTTTGGCA AGACACACAA GCTGAAGCCA GGAGTGAAGG AGCCTGTTGG TGTTAATAAA
    GCAGCACCTG CTCCACCCGT TGAGGATGTT AGCATGAGGA TCTATCTGTG TGAGGGGCTT
    CTGGGTAAAG AGCGCTCAAC TCTGTGGGAT CAGATGCAGT TCTGGGAGGA CGCCTTCCTG
    GATGCCGTAA TGCTGGAACG GGAAGGAATG GGAATGGATC AGGGACCACA AGAAATGATT
    GACAGGTATC TTTCTCTGGG AGATCATGAC CGCAAGAGAC TGGAGGATGA TGAGGACAGA
    CTGCTGGCCA CGCTTCTGCA CAATATGATT GCTTACATGC TTATGATGAA GGTCGGTAAA
    AACGACATCA GGAAGAAAGT ACGTCGTCTT ATGGGCAAAT CCCACATTGG CCTCACTCAC
    AGTCAGGAGA TCAATGAAGT CCTGGATCGC CTCGCACATT TAAGCGGTCG GGAGCTTTCT
    ATCAGGCCGA GTGGCAGCAG GCATATTAAA AAACAGACAT TTGTTGTGCA TGCTGGGACA
    GATACAACTG GTGACATATT CTTCATGGAG GTGTGTGATG ATTGCATCGT CTTGCGCAGT
    AATATTGGCA CAGTCTATGA GCGCTGGTGG TATGAGAAGC TCATAAATAT GACTTACTGT
    CCCAAAACTA AAGTGTTGTG TCTGTGGAGG AGAAACGGCC AGGAGACGCA GCTAAACAAG
    TTTTACACTA AGAAGTGTCG GGAATTGTAC TACTGTGTTA AAGACAGCAT GGAGCGTGCT
    GCTGCTCGAC AACAAAGCAT CAAACCAGGT CCAGAGCTTG GAGGCGAGTT TCCGGTTCAG
    GACATGAAGA CAGGGGAGGG TGGTCTCCTC CAAGTCACAC TGGAAGGCAT CAACCTCAAA
    TTTATGCACA GCCAGGAGCG GAAGGTTTTC ATAGAGCTGA ATCACATTAA AAAGTGCAAT
    ACAGTGAAAG GAGTCTTTGT CCTGGAGGAG TTTGTTCCTG AAACTAAAGA AGTGGTGATC
    CATAAGTACA AGACTCCTAT GGCACATCAG ATCTGTTACT CGGTGCTCTG CCTCTTCTCC
    TACATGGCGG CGGTCAAGGG GAAAGAGTCA GAGGGCAAAC CCAAGATGCT GTCCCCTCGT
    CCACTAGCGA GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48938
    Clone ID Related Accession (Same CDS sequence) XM_009303292.2
    Accession Version XM_009303292.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4656bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product MAP kinase-activating death domain protein isoform X29
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303292.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGTG TCTGGTAAGG
    TTGACTCTGA TCACCCAAAA GCAGATCTTC GCCTCCGATT CTAAGGCTCT GGCTAGCATG
    AGTCTAAACA CACAGCCTAT TCTGAACCTG GAGAAGTTTC AGGATGGACA GGAGTTGTCT
    TTATTGCCCC CCGGTCGTGA CAAAGCTTCC CCGTCCTCCA CTGAGTTCAA CCCACTCATC
    TACGGCAATG ATGTGGACTC AGTTGATGTT GCCACAAGGG TGGCAATGGT GAGGTTCTTC
    AACTCTCCAA ACGTGCTGCA AGGGTTCCAG ATGCACACAC GCACTCTCCG TCTCTTCCCT
    CGTCCTGTGG TGGCCTTTCA GTCCACATCT TTCCTGGCTT CTAGGCCACG ACGAAATGGA
    TTCACAGAAA AGCTCTCCCA CACTCAGGCA GTGGAGTACT ATGGAGAATG GGCTCTCAAC
    CCCACCAACC TGGCTTTCCA AAGGATTCAT AACAATGTGT ATGACCCCTC TCTAATAGGC
    GATAAGCCTA AGTGGTATGC CCACCAGTTA CAGCCAGTAT TCTACCGCGT GTATGATGGA
    AACTCTCATT TAGCGGAGGC CTTGAGTGGA CCCCTGCAGG ATGAGACCAA TGATTCAGAC
    CCAACAGACG ACAGTGGCAG CGACAGTGAA GCATATGATG ACTCCAGCTC CTCCTACTCA
    TCTCTTGGAG ATTTTGTGAA TGAGATGATC AAAGGGGACA TACAGGGAGA CACACCAAAT
    GTTGATCCCC TGACCCATGC TGCACTGGGA GATGCAGATG AGGTTGAGAT CCATGACTTT
    CACGAGTATA AGGGGAACAG CGGTGAACCA GAGCCAGAAG GCCCCACAGA AGCAGCGGAC
    AGTCAGCCCT TGCGGTCCAG CTCTAGCACC ACAGCCAGTT CCAGCCCCAG TACAGTCATA
    CAGGGAGTCA ACCATGAGCA GAAGGAACCT GTTGAGGTGG AGCCCATAGC AAATGTGACT
    TACCCAAGCT CTGCCCCAGT ATTGGGTCCT CCTCCTTTTA CAAGACCCAC CCCTGATCCA
    ACCCTTGTTG ACCCTGCCAA TAAGAAGCGA GAATATGACA ACCCCTATTT TGAGCCCCAG
    TACGGTTTCC CTACAGAGGA AGATGCTGAG ACTGATGAGC AGGAGGAGAG TTACACACCA
    CGATTTAACC AGAATCTCAA TGGCAATAAG CCTTCTCGTC CACTGAGACC CAGCAGCCTG
    AAGCTTCCGG GTGAATCTGA CGGAGAGGGA GACTCCAGAA ATAGCTCTCC TAACTCAACC
    ATCTCCAACA ATAGCAGTGA TGGCTTTGGG GGACTCATGT CCTTTGCCAG TAACTTGTAT
    AAAAACCATG GCACCAGTTT CAGTCTGTCC AGCCTGGCTC TGCCTAATAA AGCGAGAGAG
    AAGAACACGC CGTTTCCCAG CCTCAAAGGT GCCCGAGCCC CCAGAGCACT GGTAGATCAG
    AAATCCTCTG TTATTAAGCA CAGCCCTACT GTGAAGAGAG AGTCTCCGTC TCCTCAAGGC
    AGAGCCAACA ATACCAGTGA GAACCAGCAG TTTCTAAAGG AGGTGGTCCA GAGCGTGCTG
    GAGGGGCAGG GTGTGGGCTG GCTTAACATG AAGAAGGTGC GTCGCCTGCT AGAGAATGAA
    CAGCTCAGAG TCTTTGTGCT CAGCAAACTC AACCGTGCGG TCCAGTCAGA GGAGGACGCC
    CAGCAGGAAG TTATAAGAGA TGTGGAGATA AATAGAAAGG TCTACAAAGG CATGCTGGAT
    ATTTTGAAGT GCACGGTGTC CAGTTTGGAG CATTCATACA CTAATGCTGG CTTAGGGGGC
    ATGGCCAGTG TCTTCAGTCT GCTAGAGATA GCACGCACCC ACTACCAAAC CAAAGATCCA
    GAGAAGCGGA AACGGAGCCC CACCGAAGGG CTTAGCAGTC CAGGCAGTAA GGAGAGCCCA
    TCGGGCCGAA TGGAGAGTGC CAGGGCGGCA GGGGTGCTTC TGGTACCCCG TATCCAATTG
    CCGCCACCCT CCTCTGGGAA ATCATCACGC CAGTTTGATA CCCGGAGTCT GAACGAGGAG
    AATTTTATTG CCTCCATTGG AGCGGATGGA GTAAAACAAC GTGTAGAAGG AGGAGACACT
    GAGGAGAAGA AATCACAGAT CAGTGCAGAC AGTGGCCTCA GTGTGACATC TGGCTCACAG
    AAGAGTGATA CGGATTCCCT GGCGAGCTCT GAACCTCCTG CTCTGACCAG AAGCACCAGC
    CAGGACTCAG AGGCCAGCAC AGTGGTAAGT AACAGCTCAG GTGAGACGCT CGGTGCAGAC
    AGTGACCTGA GCAGCACTGC TGGAGATGGC CTGACAGGAA GACATGGTCA GCATCTCAAC
    CTGTCCCGTG GCACACTGTC TGACAGTGAG ATTGAGACCA ACCCTGCAAC CAGCTCAGTG
    TTTGGCAAGA CACACAAGCT GAAGCCAGGA GTGAAGGAGC CTGTTGGTGT TAATAAAGCA
    GCACCTGCTC CACCCGTTGA GGATGTTAGC ATGAGGATCT ATCTGTGTGA GGGGCTTCTG
    GGTAAAGAGC GCTCAACTCT GTGGGATCAG ATGCAGTTCT GGGAGGACGC CTTCCTGGAT
    GCCGTAATGC TGGAACGGGA AGGAATGGGA ATGGATCAGG GACCACAAGA AATGATTGAC
    AGGTATCTTT CTCTGGGAGA TCATGACCGC AAGAGACTGG AGGATGATGA GGACAGACTG
    CTGGCCACGC TTCTGCACAA TATGATTGCT TACATGCTTA TGATGAAGGT CGGTAAAAAC
    GACATCAGGA AGAAAGTACG TCGTCTTATG GGCAAATCCC ACATTGGCCT CACTCACAGT
    CAGGAGATCA ATGAAGTCCT GGATCGCCTC GCACATTTAA GCGGTCGGGA GCTTTCTATC
    AGGCCGAGTG GCAGCAGGCA TATTAAAAAA CAGACATTTG TTGTGCATGC TGGGACAGAT
    ACAACTGGTG ACATATTCTT CATGGAGGTG TGTGATGATT GCATCGTCTT GCGCAGTAAT
    ATTGGCACAG TCTATGAGCG CTGGTGGTAT GAGAAGCTCA TAAATATGAC TTACTGTCCC
    AAAACTAAAG TGTTGTGTCT GTGGAGGAGA AACGGCCAGG AGACGCAGCT AAACAAGTTT
    TACACTAAGA AGTGTCGGGA ATTGTACTAC TGTGTTAAAG ACAGCATGGA GCGTGCTGCT
    GCTCGACAAC AAAGCATCAA ACCAGGTCCA GAGCTTGGAG GCGAGTTTCC GGTTCAGGAC
    ATGAAGACAG GGGAGGGTGG TCTCCTCCAA GTCACACTGG AAGGCATCAA CCTCAAATTT
    ATGCACAGCC AGTTCCTGAA ACTAAAGAAG TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301567.1
    CDS435..5090
    Translation

    Target ORF information:

    RefSeq Version XM_009303292.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X29, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303292.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGTG TCTGGTAAGG
    TTGACTCTGA TCACCCAAAA GCAGATCTTC GCCTCCGATT CTAAGGCTCT GGCTAGCATG
    AGTCTAAACA CACAGCCTAT TCTGAACCTG GAGAAGTTTC AGGATGGACA GGAGTTGTCT
    TTATTGCCCC CCGGTCGTGA CAAAGCTTCC CCGTCCTCCA CTGAGTTCAA CCCACTCATC
    TACGGCAATG ATGTGGACTC AGTTGATGTT GCCACAAGGG TGGCAATGGT GAGGTTCTTC
    AACTCTCCAA ACGTGCTGCA AGGGTTCCAG ATGCACACAC GCACTCTCCG TCTCTTCCCT
    CGTCCTGTGG TGGCCTTTCA GTCCACATCT TTCCTGGCTT CTAGGCCACG ACGAAATGGA
    TTCACAGAAA AGCTCTCCCA CACTCAGGCA GTGGAGTACT ATGGAGAATG GGCTCTCAAC
    CCCACCAACC TGGCTTTCCA AAGGATTCAT AACAATGTGT ATGACCCCTC TCTAATAGGC
    GATAAGCCTA AGTGGTATGC CCACCAGTTA CAGCCAGTAT TCTACCGCGT GTATGATGGA
    AACTCTCATT TAGCGGAGGC CTTGAGTGGA CCCCTGCAGG ATGAGACCAA TGATTCAGAC
    CCAACAGACG ACAGTGGCAG CGACAGTGAA GCATATGATG ACTCCAGCTC CTCCTACTCA
    TCTCTTGGAG ATTTTGTGAA TGAGATGATC AAAGGGGACA TACAGGGAGA CACACCAAAT
    GTTGATCCCC TGACCCATGC TGCACTGGGA GATGCAGATG AGGTTGAGAT CCATGACTTT
    CACGAGTATA AGGGGAACAG CGGTGAACCA GAGCCAGAAG GCCCCACAGA AGCAGCGGAC
    AGTCAGCCCT TGCGGTCCAG CTCTAGCACC ACAGCCAGTT CCAGCCCCAG TACAGTCATA
    CAGGGAGTCA ACCATGAGCA GAAGGAACCT GTTGAGGTGG AGCCCATAGC AAATGTGACT
    TACCCAAGCT CTGCCCCAGT ATTGGGTCCT CCTCCTTTTA CAAGACCCAC CCCTGATCCA
    ACCCTTGTTG ACCCTGCCAA TAAGAAGCGA GAATATGACA ACCCCTATTT TGAGCCCCAG
    TACGGTTTCC CTACAGAGGA AGATGCTGAG ACTGATGAGC AGGAGGAGAG TTACACACCA
    CGATTTAACC AGAATCTCAA TGGCAATAAG CCTTCTCGTC CACTGAGACC CAGCAGCCTG
    AAGCTTCCGG GTGAATCTGA CGGAGAGGGA GACTCCAGAA ATAGCTCTCC TAACTCAACC
    ATCTCCAACA ATAGCAGTGA TGGCTTTGGG GGACTCATGT CCTTTGCCAG TAACTTGTAT
    AAAAACCATG GCACCAGTTT CAGTCTGTCC AGCCTGGCTC TGCCTAATAA AGCGAGAGAG
    AAGAACACGC CGTTTCCCAG CCTCAAAGGT GCCCGAGCCC CCAGAGCACT GGTAGATCAG
    AAATCCTCTG TTATTAAGCA CAGCCCTACT GTGAAGAGAG AGTCTCCGTC TCCTCAAGGC
    AGAGCCAACA ATACCAGTGA GAACCAGCAG TTTCTAAAGG AGGTGGTCCA GAGCGTGCTG
    GAGGGGCAGG GTGTGGGCTG GCTTAACATG AAGAAGGTGC GTCGCCTGCT AGAGAATGAA
    CAGCTCAGAG TCTTTGTGCT CAGCAAACTC AACCGTGCGG TCCAGTCAGA GGAGGACGCC
    CAGCAGGAAG TTATAAGAGA TGTGGAGATA AATAGAAAGG TCTACAAAGG CATGCTGGAT
    ATTTTGAAGT GCACGGTGTC CAGTTTGGAG CATTCATACA CTAATGCTGG CTTAGGGGGC
    ATGGCCAGTG TCTTCAGTCT GCTAGAGATA GCACGCACCC ACTACCAAAC CAAAGATCCA
    GAGAAGCGGA AACGGAGCCC CACCGAAGGG CTTAGCAGTC CAGGCAGTAA GGAGAGCCCA
    TCGGGCCGAA TGGAGAGTGC CAGGGCGGCA GGGGTGCTTC TGGTACCCCG TATCCAATTG
    CCGCCACCCT CCTCTGGGAA ATCATCACGC CAGTTTGATA CCCGGAGTCT GAACGAGGAG
    AATTTTATTG CCTCCATTGG AGCGGATGGA GTAAAACAAC GTGTAGAAGG AGGAGACACT
    GAGGAGAAGA AATCACAGAT CAGTGCAGAC AGTGGCCTCA GTGTGACATC TGGCTCACAG
    AAGAGTGATA CGGATTCCCT GGCGAGCTCT GAACCTCCTG CTCTGACCAG AAGCACCAGC
    CAGGACTCAG AGGCCAGCAC AGTGGTAAGT AACAGCTCAG GTGAGACGCT CGGTGCAGAC
    AGTGACCTGA GCAGCACTGC TGGAGATGGC CTGACAGGAA GACATGGTCA GCATCTCAAC
    CTGTCCCGTG GCACACTGTC TGACAGTGAG ATTGAGACCA ACCCTGCAAC CAGCTCAGTG
    TTTGGCAAGA CACACAAGCT GAAGCCAGGA GTGAAGGAGC CTGTTGGTGT TAATAAAGCA
    GCACCTGCTC CACCCGTTGA GGATGTTAGC ATGAGGATCT ATCTGTGTGA GGGGCTTCTG
    GGTAAAGAGC GCTCAACTCT GTGGGATCAG ATGCAGTTCT GGGAGGACGC CTTCCTGGAT
    GCCGTAATGC TGGAACGGGA AGGAATGGGA ATGGATCAGG GACCACAAGA AATGATTGAC
    AGGTATCTTT CTCTGGGAGA TCATGACCGC AAGAGACTGG AGGATGATGA GGACAGACTG
    CTGGCCACGC TTCTGCACAA TATGATTGCT TACATGCTTA TGATGAAGGT CGGTAAAAAC
    GACATCAGGA AGAAAGTACG TCGTCTTATG GGCAAATCCC ACATTGGCCT CACTCACAGT
    CAGGAGATCA ATGAAGTCCT GGATCGCCTC GCACATTTAA GCGGTCGGGA GCTTTCTATC
    AGGCCGAGTG GCAGCAGGCA TATTAAAAAA CAGACATTTG TTGTGCATGC TGGGACAGAT
    ACAACTGGTG ACATATTCTT CATGGAGGTG TGTGATGATT GCATCGTCTT GCGCAGTAAT
    ATTGGCACAG TCTATGAGCG CTGGTGGTAT GAGAAGCTCA TAAATATGAC TTACTGTCCC
    AAAACTAAAG TGTTGTGTCT GTGGAGGAGA AACGGCCAGG AGACGCAGCT AAACAAGTTT
    TACACTAAGA AGTGTCGGGA ATTGTACTAC TGTGTTAAAG ACAGCATGGA GCGTGCTGCT
    GCTCGACAAC AAAGCATCAA ACCAGGTCCA GAGCTTGGAG GCGAGTTTCC GGTTCAGGAC
    ATGAAGACAG GGGAGGGTGG TCTCCTCCAA GTCACACTGG AAGGCATCAA CCTCAAATTT
    ATGCACAGCC AGTTCCTGAA ACTAAAGAAG TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48939
    Clone ID Related Accession (Same CDS sequence) XM_009303293.2
    Accession Version XM_009303293.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5010bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product MAP kinase-activating death domain protein isoform X30
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303293.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GTATTTGGGC TAAATTCTCT AATGGAGATT
    GTAACAGAGA TCGGCCCGGG GAGCGGAGAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGAATTGTGG AGCAAGCACC AGGATAACAG AAAGCAAAAA
    GCTTTGGAAA AAGAACAGAG TGGTTTAAAT AAACGGAAAT CAGGAGCGGA TGGAGTAAAA
    CAACGTGTAG AAGGAGGAGA CACTGAGGAG AAGAAATCAC AGATCAGTGC AGACAGTGGC
    CTCAGTGTGA CATCTGGCTC ACAGAAGAGT GATACGGATT CCCTGGCGAG CTCTGAACCT
    CCTGCTCTGA CCAGAAGCAC CAGCCAGGAC TCAGAGGCCA GCACAGTGGT AAGTAACAGC
    TCAGGTGAGA CGCTCGGTGC AGACAGTGAC CTGAGCAGCA CTGCTGGAGA TGGCCTGACA
    GGAAGACATG GTCAGCATCT CAACCTGTCC CGTGGCACAC TGTCTGACAG TGAGATTGAG
    ACCAACCCTG CAACCAGCTC AGTGTTTGGC AAGACACACA AGCTGAAGCC AGGAGTGAAG
    GAGCCTGTTG GTGTTAATAA AGCAGCACCT GCTCCACCCG TTGAGGATGT TAGCATGAGG
    ATCTATCTGT GTGAGGGGCT TCTGGGCCGG GATAAAAGCT CAGTTTGGGA CCAGTTGGAG
    GATGCTGCAA TGGAAACCTT TTCTCTGAGT AAAGAGCGCT CAACTCTGTG GGATCAGATG
    CAGTTCTGGG AGGACGCCTT CCTGGATGCC GTAATGCTGG AACGGGAAGG AATGGGAATG
    GATCAGGGAC CACAAGAAAT GATTGACAGG TATCTTTCTC TGGGAGATCA TGACCGCAAG
    AGACTGGAGG ATGATGAGGA CAGACTGCTG GCCACGCTTC TGCACAATAT GATTGCTTAC
    ATGCTTATGA TGAAGGTCGG TAAAAACGAC ATCAGGAAGA AAGTACGTCG TCTTATGGGC
    AAATCCCACA TTGGCCTCAC TCACAGTCAG GAGATCAATG AAGTCCTGGA TCGCCTCGCA
    CATTTAAGCG GTCGGGAGCT TTCTATCAGG CCGAGTGGCA GCAGGCATAT TAAAAAACAG
    ACATTTGTTG TGCATGCTGG GACAGATACA ACTGGTGACA TATTCTTCAT GGAGGTGTGT
    GATGATTGCA TCGTCTTGCG CAGTAATATT GGCACAGTCT ATGAGCGCTG GTGGTATGAG
    AAGCTCATAA ATATGACTTA CTGTCCCAAA ACTAAAGTGT TGTGTCTGTG GAGGAGAAAC
    GGCCAGGAGA CGCAGCTAAA CAAGTTTTAC ACTAAGAAGT GTCGGGAATT GTACTACTGT
    GTTAAAGACA GCATGGAGCG TGCTGCTGCT CGACAACAAA GCATCAAACC AGGTCCAGAG
    CTTGGAGGCG AGTTTCCGGT TCAGGACATG AAGACAGGGG AGGGTGGTCT CCTCCAAGTC
    ACACTGGAAG GCATCAACCT CAAATTTATG CACAGCCAGG TTTTCATAGA GCTGAATCAC
    ATTAAAAAGT GCAATACAGT GAAAGGAGTC TTTGTCCTGG AGGAGTTTGT TCCTGAAACT
    AAAGAAGTGG TGATCCATAA GTACAAGACT CCTATGGCAC ATCAGATCTG TTACTCGGTG
    CTCTGCCTCT TCTCCTACAT GGCGGCGGTC AAGGGGAAAG AGTCAGAGGG CAAACCCAAG
    ATGCTGTCCC CTCGTCCACT AGCGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301568.1
    CDS435..5444
    Translation

    Target ORF information:

    RefSeq Version XM_009303293.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X30, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303293.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GTATTTGGGC TAAATTCTCT AATGGAGATT
    GTAACAGAGA TCGGCCCGGG GAGCGGAGAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGAATTGTGG AGCAAGCACC AGGATAACAG AAAGCAAAAA
    GCTTTGGAAA AAGAACAGAG TGGTTTAAAT AAACGGAAAT CAGGAGCGGA TGGAGTAAAA
    CAACGTGTAG AAGGAGGAGA CACTGAGGAG AAGAAATCAC AGATCAGTGC AGACAGTGGC
    CTCAGTGTGA CATCTGGCTC ACAGAAGAGT GATACGGATT CCCTGGCGAG CTCTGAACCT
    CCTGCTCTGA CCAGAAGCAC CAGCCAGGAC TCAGAGGCCA GCACAGTGGT AAGTAACAGC
    TCAGGTGAGA CGCTCGGTGC AGACAGTGAC CTGAGCAGCA CTGCTGGAGA TGGCCTGACA
    GGAAGACATG GTCAGCATCT CAACCTGTCC CGTGGCACAC TGTCTGACAG TGAGATTGAG
    ACCAACCCTG CAACCAGCTC AGTGTTTGGC AAGACACACA AGCTGAAGCC AGGAGTGAAG
    GAGCCTGTTG GTGTTAATAA AGCAGCACCT GCTCCACCCG TTGAGGATGT TAGCATGAGG
    ATCTATCTGT GTGAGGGGCT TCTGGGCCGG GATAAAAGCT CAGTTTGGGA CCAGTTGGAG
    GATGCTGCAA TGGAAACCTT TTCTCTGAGT AAAGAGCGCT CAACTCTGTG GGATCAGATG
    CAGTTCTGGG AGGACGCCTT CCTGGATGCC GTAATGCTGG AACGGGAAGG AATGGGAATG
    GATCAGGGAC CACAAGAAAT GATTGACAGG TATCTTTCTC TGGGAGATCA TGACCGCAAG
    AGACTGGAGG ATGATGAGGA CAGACTGCTG GCCACGCTTC TGCACAATAT GATTGCTTAC
    ATGCTTATGA TGAAGGTCGG TAAAAACGAC ATCAGGAAGA AAGTACGTCG TCTTATGGGC
    AAATCCCACA TTGGCCTCAC TCACAGTCAG GAGATCAATG AAGTCCTGGA TCGCCTCGCA
    CATTTAAGCG GTCGGGAGCT TTCTATCAGG CCGAGTGGCA GCAGGCATAT TAAAAAACAG
    ACATTTGTTG TGCATGCTGG GACAGATACA ACTGGTGACA TATTCTTCAT GGAGGTGTGT
    GATGATTGCA TCGTCTTGCG CAGTAATATT GGCACAGTCT ATGAGCGCTG GTGGTATGAG
    AAGCTCATAA ATATGACTTA CTGTCCCAAA ACTAAAGTGT TGTGTCTGTG GAGGAGAAAC
    GGCCAGGAGA CGCAGCTAAA CAAGTTTTAC ACTAAGAAGT GTCGGGAATT GTACTACTGT
    GTTAAAGACA GCATGGAGCG TGCTGCTGCT CGACAACAAA GCATCAAACC AGGTCCAGAG
    CTTGGAGGCG AGTTTCCGGT TCAGGACATG AAGACAGGGG AGGGTGGTCT CCTCCAAGTC
    ACACTGGAAG GCATCAACCT CAAATTTATG CACAGCCAGG TTTTCATAGA GCTGAATCAC
    ATTAAAAAGT GCAATACAGT GAAAGGAGTC TTTGTCCTGG AGGAGTTTGT TCCTGAAACT
    AAAGAAGTGG TGATCCATAA GTACAAGACT CCTATGGCAC ATCAGATCTG TTACTCGGTG
    CTCTGCCTCT TCTCCTACAT GGCGGCGGTC AAGGGGAAAG AGTCAGAGGG CAAACCCAAG
    ATGCTGTCCC CTCGTCCACT AGCGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48940
    Clone ID Related Accession (Same CDS sequence) XM_009303294.3 , XM_009303294.2
    Accession Version XM_009303294.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4803bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X34
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303294.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GTATTTGGGC TAAATTCTCT AATGGAGATT
    GTAACAGAGA TCGGCCCGGG GAGCGGAGAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGAATTGTGG AGCAAGCACC AGGATAACAG AAAGCAAAAA
    GCTTTGGAAA AAGAACAGAG TGGTTTAAAT AAACGGAAAT CAGGAGCGGA TGGAGTAAAA
    CAACGTGTAG AAGGAGGAGA CACTGAGGAG AAGAAATCAC AGATCAGTGC AGACAGTGGC
    CTCAGTGTGA CATCTGGCTC ACAGAAGAGT GATACGGATT CCCTGGCGAG CTCTGAACCT
    CCTGCTCTGA CCAGAAGCAC CAGCCAGGAC TCAGAGGCCA GCACAGTGGT AAGTAACAGC
    TCAGGTGAGA CGCTCGGTGC AGACAGTGAC CTGAGCAGCA CTGCTGGAGA TGGCCTGACA
    GGAAGACATG GTCAGCATCT CAACCTGTCC CGTGGCACAC TGTCTGACAG TGAGATTGAG
    ACCAACCCTG CAACCAGCTC AGTGTTTGGC AAGACACACA AGCTGAAGCC AGGAGTGAAG
    GAGCCTGTTG GTGTTAATAA AGCAGCACCT GCTCCACCCG TTGAGGATGT TAGCATGAGG
    ATCTATCTGT GTGAGGGGCT TCTGGGCCGG GATAAAAGCT CAGTTTGGGA CCAGTTGGAG
    GATGCTGCAA TGGAAACCTT TTCTCTGAGT AAAGAGCGCT CAACTCTGTG GGATCAGATG
    CAGTTCTGGG AGGACGCCTT CCTGGATGCC GTAATGCTGG AACGGGAAGG AATGGGAATG
    GATCAGGGAC CACAAGAAAT GATTGACAGG TATCTTTCTC TGGGAGATCA TGACCGCAAG
    AGACTGGAGG ATGATGAGGA CAGACTGCTG GCCACGCTTC TGCACAATAT GATTGCTTAC
    ATGCTTATGA TGAAGGTCGG TAAAAACGAC ATCAGGAAGA AAGTACGTCG TCTTATGGGC
    AAATCCCACA TTGGCCTCAC TCACAGTCAG GAGATCAATG AAGTCCTGGA TCGCCTCGCA
    CATTTAAGCG GTCGGGAGCT TTCTATCAGG CCGAGTGGCA GCAGGCATAT TAAAAAACAG
    ACATTTGTTG TGCATGCTGG GACAGATACA ACTGGTGACA TATTCTTCAT GGAGGTGTGT
    GATGATTGCA TCGTCTTGCG CAGTAATATT GGCACAGTCT ATGAGCGCTG GTGGTATGAG
    AAGCTCATAA ATATGACTTA CTGTCCCAAA ACTAAAGTGT TGTGTCTGTG GAGGAGAAAC
    GGCCAGGAGA CGCAGCTAAA CAAGTTTTAC ACTAAGAAGT GTCGGGAATT GTACTACTGT
    GTTAAAGACA GCATGGAGCG TGCTGCTGCT CGACAACAAA GCATCAAACC AGGTCCAGAG
    CTTGGAGGCG AGTTTCCGGT TCAGGACATG AAGACAGGGG AGGGTGGTCT CCTCCAAGTC
    ACACTGGAAG GCATCAACCT CAAATTTATG CACAGCCAGT TCCTGAAACT AAAGAAGTGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301569.1
    CDS432..5234
    Translation

    Target ORF information:

    RefSeq Version XM_009303294.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X35, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303294.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GTATTTGGGC TAAATTCTCT AATGGAGATT
    GTAACAGAGA TCGGCCCGGG GAGCGGAGAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGAATTGTGG AGCAAGCACC AGGATAACAG AAAGCAAAAA
    GCTTTGGAAA AAGAACAGAG TGGTTTAAAT AAACGGAAAT CAGGAGCGGA TGGAGTAAAA
    CAACGTGTAG AAGGAGGAGA CACTGAGGAG AAGAAATCAC AGATCAGTGC AGACAGTGGC
    CTCAGTGTGA CATCTGGCTC ACAGAAGAGT GATACGGATT CCCTGGCGAG CTCTGAACCT
    CCTGCTCTGA CCAGAAGCAC CAGCCAGGAC TCAGAGGCCA GCACAGTGGT AAGTAACAGC
    TCAGGTGAGA CGCTCGGTGC AGACAGTGAC CTGAGCAGCA CTGCTGGAGA TGGCCTGACA
    GGAAGACATG GTCAGCATCT CAACCTGTCC CGTGGCACAC TGTCTGACAG TGAGATTGAG
    ACCAACCCTG CAACCAGCTC AGTGTTTGGC AAGACACACA AGCTGAAGCC AGGAGTGAAG
    GAGCCTGTTG GTGTTAATAA AGCAGCACCT GCTCCACCCG TTGAGGATGT TAGCATGAGG
    ATCTATCTGT GTGAGGGGCT TCTGGGCCGG GATAAAAGCT CAGTTTGGGA CCAGTTGGAG
    GATGCTGCAA TGGAAACCTT TTCTCTGAGT AAAGAGCGCT CAACTCTGTG GGATCAGATG
    CAGTTCTGGG AGGACGCCTT CCTGGATGCC GTAATGCTGG AACGGGAAGG AATGGGAATG
    GATCAGGGAC CACAAGAAAT GATTGACAGG TATCTTTCTC TGGGAGATCA TGACCGCAAG
    AGACTGGAGG ATGATGAGGA CAGACTGCTG GCCACGCTTC TGCACAATAT GATTGCTTAC
    ATGCTTATGA TGAAGGTCGG TAAAAACGAC ATCAGGAAGA AAGTACGTCG TCTTATGGGC
    AAATCCCACA TTGGCCTCAC TCACAGTCAG GAGATCAATG AAGTCCTGGA TCGCCTCGCA
    CATTTAAGCG GTCGGGAGCT TTCTATCAGG CCGAGTGGCA GCAGGCATAT TAAAAAACAG
    ACATTTGTTG TGCATGCTGG GACAGATACA ACTGGTGACA TATTCTTCAT GGAGGTGTGT
    GATGATTGCA TCGTCTTGCG CAGTAATATT GGCACAGTCT ATGAGCGCTG GTGGTATGAG
    AAGCTCATAA ATATGACTTA CTGTCCCAAA ACTAAAGTGT TGTGTCTGTG GAGGAGAAAC
    GGCCAGGAGA CGCAGCTAAA CAAGTTTTAC ACTAAGAAGT GTCGGGAATT GTACTACTGT
    GTTAAAGACA GCATGGAGCG TGCTGCTGCT CGACAACAAA GCATCAAACC AGGTCCAGAG
    CTTGGAGGCG AGTTTCCGGT TCAGGACATG AAGACAGGGG AGGGTGGTCT CCTCCAAGTC
    ACACTGGAAG GCATCAACCT CAAATTTATG CACAGCCAGT TCCTGAAACT AAAGAAGTGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48940
    Clone ID Related Accession (Same CDS sequence) XM_009303294.3 , XM_009303294.2
    Accession Version XM_009303294.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4803bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product MAP kinase-activating death domain protein isoform X31
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303294.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GTATTTGGGC TAAATTCTCT AATGGAGATT
    GTAACAGAGA TCGGCCCGGG GAGCGGAGAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGAATTGTGG AGCAAGCACC AGGATAACAG AAAGCAAAAA
    GCTTTGGAAA AAGAACAGAG TGGTTTAAAT AAACGGAAAT CAGGAGCGGA TGGAGTAAAA
    CAACGTGTAG AAGGAGGAGA CACTGAGGAG AAGAAATCAC AGATCAGTGC AGACAGTGGC
    CTCAGTGTGA CATCTGGCTC ACAGAAGAGT GATACGGATT CCCTGGCGAG CTCTGAACCT
    CCTGCTCTGA CCAGAAGCAC CAGCCAGGAC TCAGAGGCCA GCACAGTGGT AAGTAACAGC
    TCAGGTGAGA CGCTCGGTGC AGACAGTGAC CTGAGCAGCA CTGCTGGAGA TGGCCTGACA
    GGAAGACATG GTCAGCATCT CAACCTGTCC CGTGGCACAC TGTCTGACAG TGAGATTGAG
    ACCAACCCTG CAACCAGCTC AGTGTTTGGC AAGACACACA AGCTGAAGCC AGGAGTGAAG
    GAGCCTGTTG GTGTTAATAA AGCAGCACCT GCTCCACCCG TTGAGGATGT TAGCATGAGG
    ATCTATCTGT GTGAGGGGCT TCTGGGCCGG GATAAAAGCT CAGTTTGGGA CCAGTTGGAG
    GATGCTGCAA TGGAAACCTT TTCTCTGAGT AAAGAGCGCT CAACTCTGTG GGATCAGATG
    CAGTTCTGGG AGGACGCCTT CCTGGATGCC GTAATGCTGG AACGGGAAGG AATGGGAATG
    GATCAGGGAC CACAAGAAAT GATTGACAGG TATCTTTCTC TGGGAGATCA TGACCGCAAG
    AGACTGGAGG ATGATGAGGA CAGACTGCTG GCCACGCTTC TGCACAATAT GATTGCTTAC
    ATGCTTATGA TGAAGGTCGG TAAAAACGAC ATCAGGAAGA AAGTACGTCG TCTTATGGGC
    AAATCCCACA TTGGCCTCAC TCACAGTCAG GAGATCAATG AAGTCCTGGA TCGCCTCGCA
    CATTTAAGCG GTCGGGAGCT TTCTATCAGG CCGAGTGGCA GCAGGCATAT TAAAAAACAG
    ACATTTGTTG TGCATGCTGG GACAGATACA ACTGGTGACA TATTCTTCAT GGAGGTGTGT
    GATGATTGCA TCGTCTTGCG CAGTAATATT GGCACAGTCT ATGAGCGCTG GTGGTATGAG
    AAGCTCATAA ATATGACTTA CTGTCCCAAA ACTAAAGTGT TGTGTCTGTG GAGGAGAAAC
    GGCCAGGAGA CGCAGCTAAA CAAGTTTTAC ACTAAGAAGT GTCGGGAATT GTACTACTGT
    GTTAAAGACA GCATGGAGCG TGCTGCTGCT CGACAACAAA GCATCAAACC AGGTCCAGAG
    CTTGGAGGCG AGTTTCCGGT TCAGGACATG AAGACAGGGG AGGGTGGTCT CCTCCAAGTC
    ACACTGGAAG GCATCAACCT CAAATTTATG CACAGCCAGT TCCTGAAACT AAAGAAGTGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301569.1
    CDS435..5237
    Translation

    Target ORF information:

    RefSeq Version XM_009303294.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X31, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303294.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GTATTTGGGC TAAATTCTCT AATGGAGATT
    GTAACAGAGA TCGGCCCGGG GAGCGGAGAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGAATTGTGG AGCAAGCACC AGGATAACAG AAAGCAAAAA
    GCTTTGGAAA AAGAACAGAG TGGTTTAAAT AAACGGAAAT CAGGAGCGGA TGGAGTAAAA
    CAACGTGTAG AAGGAGGAGA CACTGAGGAG AAGAAATCAC AGATCAGTGC AGACAGTGGC
    CTCAGTGTGA CATCTGGCTC ACAGAAGAGT GATACGGATT CCCTGGCGAG CTCTGAACCT
    CCTGCTCTGA CCAGAAGCAC CAGCCAGGAC TCAGAGGCCA GCACAGTGGT AAGTAACAGC
    TCAGGTGAGA CGCTCGGTGC AGACAGTGAC CTGAGCAGCA CTGCTGGAGA TGGCCTGACA
    GGAAGACATG GTCAGCATCT CAACCTGTCC CGTGGCACAC TGTCTGACAG TGAGATTGAG
    ACCAACCCTG CAACCAGCTC AGTGTTTGGC AAGACACACA AGCTGAAGCC AGGAGTGAAG
    GAGCCTGTTG GTGTTAATAA AGCAGCACCT GCTCCACCCG TTGAGGATGT TAGCATGAGG
    ATCTATCTGT GTGAGGGGCT TCTGGGCCGG GATAAAAGCT CAGTTTGGGA CCAGTTGGAG
    GATGCTGCAA TGGAAACCTT TTCTCTGAGT AAAGAGCGCT CAACTCTGTG GGATCAGATG
    CAGTTCTGGG AGGACGCCTT CCTGGATGCC GTAATGCTGG AACGGGAAGG AATGGGAATG
    GATCAGGGAC CACAAGAAAT GATTGACAGG TATCTTTCTC TGGGAGATCA TGACCGCAAG
    AGACTGGAGG ATGATGAGGA CAGACTGCTG GCCACGCTTC TGCACAATAT GATTGCTTAC
    ATGCTTATGA TGAAGGTCGG TAAAAACGAC ATCAGGAAGA AAGTACGTCG TCTTATGGGC
    AAATCCCACA TTGGCCTCAC TCACAGTCAG GAGATCAATG AAGTCCTGGA TCGCCTCGCA
    CATTTAAGCG GTCGGGAGCT TTCTATCAGG CCGAGTGGCA GCAGGCATAT TAAAAAACAG
    ACATTTGTTG TGCATGCTGG GACAGATACA ACTGGTGACA TATTCTTCAT GGAGGTGTGT
    GATGATTGCA TCGTCTTGCG CAGTAATATT GGCACAGTCT ATGAGCGCTG GTGGTATGAG
    AAGCTCATAA ATATGACTTA CTGTCCCAAA ACTAAAGTGT TGTGTCTGTG GAGGAGAAAC
    GGCCAGGAGA CGCAGCTAAA CAAGTTTTAC ACTAAGAAGT GTCGGGAATT GTACTACTGT
    GTTAAAGACA GCATGGAGCG TGCTGCTGCT CGACAACAAA GCATCAAACC AGGTCCAGAG
    CTTGGAGGCG AGTTTCCGGT TCAGGACATG AAGACAGGGG AGGGTGGTCT CCTCCAAGTC
    ACACTGGAAG GCATCAACCT CAAATTTATG CACAGCCAGT TCCTGAAACT AAAGAAGTGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48941
    Clone ID Related Accession (Same CDS sequence) XM_009303295.2 , XM_009303295.3
    Accession Version XM_009303295.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4800bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product MAP kinase-activating death domain protein isoform X32
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303295.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GTATTTGGGC TAAATTCTCT AATGGAGATT
    GTAACAGAGA TCGGCCCGGG GAGCGGAGAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGAATTGTGG AGCAAGCACC AGGATAACAG AAAGCAAAAA
    GCTTTGGAAA AAGAACAGAG TGGTTTAAAT AAACGGAAAT CAGGAGCGGA TGGAGTAAAA
    CAACGTGTAG AAGGAGGAGA CACTGAGGAG AAGAAATCAC AGATCAGTGC AGACAGTGGC
    CTCAGTGTGA CATCTGGCTC ACAGAAGAGT GATACGGATT CCCTGGCGAG CTCTGAACCT
    CCTGCTCTGA CCAGAAGCAC CAGCCAGGAC TCAGAGGCCA GCACAGTAAG TAACAGCTCA
    GGTGAGACGC TCGGTGCAGA CAGTGACCTG AGCAGCACTG CTGGAGATGG CCTGACAGGA
    AGACATGGTC AGCATCTCAA CCTGTCCCGT GGCACACTGT CTGACAGTGA GATTGAGACC
    AACCCTGCAA CCAGCTCAGT GTTTGGCAAG ACACACAAGC TGAAGCCAGG AGTGAAGGAG
    CCTGTTGGTG TTAATAAAGC AGCACCTGCT CCACCCGTTG AGGATGTTAG CATGAGGATC
    TATCTGTGTG AGGGGCTTCT GGGCCGGGAT AAAAGCTCAG TTTGGGACCA GTTGGAGGAT
    GCTGCAATGG AAACCTTTTC TCTGAGTAAA GAGCGCTCAA CTCTGTGGGA TCAGATGCAG
    TTCTGGGAGG ACGCCTTCCT GGATGCCGTA ATGCTGGAAC GGGAAGGAAT GGGAATGGAT
    CAGGGACCAC AAGAAATGAT TGACAGGTAT CTTTCTCTGG GAGATCATGA CCGCAAGAGA
    CTGGAGGATG ATGAGGACAG ACTGCTGGCC ACGCTTCTGC ACAATATGAT TGCTTACATG
    CTTATGATGA AGGTCGGTAA AAACGACATC AGGAAGAAAG TACGTCGTCT TATGGGCAAA
    TCCCACATTG GCCTCACTCA CAGTCAGGAG ATCAATGAAG TCCTGGATCG CCTCGCACAT
    TTAAGCGGTC GGGAGCTTTC TATCAGGCCG AGTGGCAGCA GGCATATTAA AAAACAGACA
    TTTGTTGTGC ATGCTGGGAC AGATACAACT GGTGACATAT TCTTCATGGA GGTGTGTGAT
    GATTGCATCG TCTTGCGCAG TAATATTGGC ACAGTCTATG AGCGCTGGTG GTATGAGAAG
    CTCATAAATA TGACTTACTG TCCCAAAACT AAAGTGTTGT GTCTGTGGAG GAGAAACGGC
    CAGGAGACGC AGCTAAACAA GTTTTACACT AAGAAGTGTC GGGAATTGTA CTACTGTGTT
    AAAGACAGCA TGGAGCGTGC TGCTGCTCGA CAACAAAGCA TCAAACCAGG TCCAGAGCTT
    GGAGGCGAGT TTCCGGTTCA GGACATGAAG ACAGGGGAGG GTGGTCTCCT CCAAGTCACA
    CTGGAAGGCA TCAACCTCAA ATTTATGCAC AGCCAGTTCC TGAAACTAAA GAAGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301570.1
    CDS435..5234
    Translation

    Target ORF information:

    RefSeq Version XM_009303295.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X32, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303295.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GTATTTGGGC TAAATTCTCT AATGGAGATT
    GTAACAGAGA TCGGCCCGGG GAGCGGAGAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGAATTGTGG AGCAAGCACC AGGATAACAG AAAGCAAAAA
    GCTTTGGAAA AAGAACAGAG TGGTTTAAAT AAACGGAAAT CAGGAGCGGA TGGAGTAAAA
    CAACGTGTAG AAGGAGGAGA CACTGAGGAG AAGAAATCAC AGATCAGTGC AGACAGTGGC
    CTCAGTGTGA CATCTGGCTC ACAGAAGAGT GATACGGATT CCCTGGCGAG CTCTGAACCT
    CCTGCTCTGA CCAGAAGCAC CAGCCAGGAC TCAGAGGCCA GCACAGTAAG TAACAGCTCA
    GGTGAGACGC TCGGTGCAGA CAGTGACCTG AGCAGCACTG CTGGAGATGG CCTGACAGGA
    AGACATGGTC AGCATCTCAA CCTGTCCCGT GGCACACTGT CTGACAGTGA GATTGAGACC
    AACCCTGCAA CCAGCTCAGT GTTTGGCAAG ACACACAAGC TGAAGCCAGG AGTGAAGGAG
    CCTGTTGGTG TTAATAAAGC AGCACCTGCT CCACCCGTTG AGGATGTTAG CATGAGGATC
    TATCTGTGTG AGGGGCTTCT GGGCCGGGAT AAAAGCTCAG TTTGGGACCA GTTGGAGGAT
    GCTGCAATGG AAACCTTTTC TCTGAGTAAA GAGCGCTCAA CTCTGTGGGA TCAGATGCAG
    TTCTGGGAGG ACGCCTTCCT GGATGCCGTA ATGCTGGAAC GGGAAGGAAT GGGAATGGAT
    CAGGGACCAC AAGAAATGAT TGACAGGTAT CTTTCTCTGG GAGATCATGA CCGCAAGAGA
    CTGGAGGATG ATGAGGACAG ACTGCTGGCC ACGCTTCTGC ACAATATGAT TGCTTACATG
    CTTATGATGA AGGTCGGTAA AAACGACATC AGGAAGAAAG TACGTCGTCT TATGGGCAAA
    TCCCACATTG GCCTCACTCA CAGTCAGGAG ATCAATGAAG TCCTGGATCG CCTCGCACAT
    TTAAGCGGTC GGGAGCTTTC TATCAGGCCG AGTGGCAGCA GGCATATTAA AAAACAGACA
    TTTGTTGTGC ATGCTGGGAC AGATACAACT GGTGACATAT TCTTCATGGA GGTGTGTGAT
    GATTGCATCG TCTTGCGCAG TAATATTGGC ACAGTCTATG AGCGCTGGTG GTATGAGAAG
    CTCATAAATA TGACTTACTG TCCCAAAACT AAAGTGTTGT GTCTGTGGAG GAGAAACGGC
    CAGGAGACGC AGCTAAACAA GTTTTACACT AAGAAGTGTC GGGAATTGTA CTACTGTGTT
    AAAGACAGCA TGGAGCGTGC TGCTGCTCGA CAACAAAGCA TCAAACCAGG TCCAGAGCTT
    GGAGGCGAGT TTCCGGTTCA GGACATGAAG ACAGGGGAGG GTGGTCTCCT CCAAGTCACA
    CTGGAAGGCA TCAACCTCAA ATTTATGCAC AGCCAGTTCC TGAAACTAAA GAAGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48941
    Clone ID Related Accession (Same CDS sequence) XM_009303295.2 , XM_009303295.3
    Accession Version XM_009303295.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4800bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X35
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303295.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GTATTTGGGC TAAATTCTCT AATGGAGATT
    GTAACAGAGA TCGGCCCGGG GAGCGGAGAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGAATTGTGG AGCAAGCACC AGGATAACAG AAAGCAAAAA
    GCTTTGGAAA AAGAACAGAG TGGTTTAAAT AAACGGAAAT CAGGAGCGGA TGGAGTAAAA
    CAACGTGTAG AAGGAGGAGA CACTGAGGAG AAGAAATCAC AGATCAGTGC AGACAGTGGC
    CTCAGTGTGA CATCTGGCTC ACAGAAGAGT GATACGGATT CCCTGGCGAG CTCTGAACCT
    CCTGCTCTGA CCAGAAGCAC CAGCCAGGAC TCAGAGGCCA GCACAGTAAG TAACAGCTCA
    GGTGAGACGC TCGGTGCAGA CAGTGACCTG AGCAGCACTG CTGGAGATGG CCTGACAGGA
    AGACATGGTC AGCATCTCAA CCTGTCCCGT GGCACACTGT CTGACAGTGA GATTGAGACC
    AACCCTGCAA CCAGCTCAGT GTTTGGCAAG ACACACAAGC TGAAGCCAGG AGTGAAGGAG
    CCTGTTGGTG TTAATAAAGC AGCACCTGCT CCACCCGTTG AGGATGTTAG CATGAGGATC
    TATCTGTGTG AGGGGCTTCT GGGCCGGGAT AAAAGCTCAG TTTGGGACCA GTTGGAGGAT
    GCTGCAATGG AAACCTTTTC TCTGAGTAAA GAGCGCTCAA CTCTGTGGGA TCAGATGCAG
    TTCTGGGAGG ACGCCTTCCT GGATGCCGTA ATGCTGGAAC GGGAAGGAAT GGGAATGGAT
    CAGGGACCAC AAGAAATGAT TGACAGGTAT CTTTCTCTGG GAGATCATGA CCGCAAGAGA
    CTGGAGGATG ATGAGGACAG ACTGCTGGCC ACGCTTCTGC ACAATATGAT TGCTTACATG
    CTTATGATGA AGGTCGGTAA AAACGACATC AGGAAGAAAG TACGTCGTCT TATGGGCAAA
    TCCCACATTG GCCTCACTCA CAGTCAGGAG ATCAATGAAG TCCTGGATCG CCTCGCACAT
    TTAAGCGGTC GGGAGCTTTC TATCAGGCCG AGTGGCAGCA GGCATATTAA AAAACAGACA
    TTTGTTGTGC ATGCTGGGAC AGATACAACT GGTGACATAT TCTTCATGGA GGTGTGTGAT
    GATTGCATCG TCTTGCGCAG TAATATTGGC ACAGTCTATG AGCGCTGGTG GTATGAGAAG
    CTCATAAATA TGACTTACTG TCCCAAAACT AAAGTGTTGT GTCTGTGGAG GAGAAACGGC
    CAGGAGACGC AGCTAAACAA GTTTTACACT AAGAAGTGTC GGGAATTGTA CTACTGTGTT
    AAAGACAGCA TGGAGCGTGC TGCTGCTCGA CAACAAAGCA TCAAACCAGG TCCAGAGCTT
    GGAGGCGAGT TTCCGGTTCA GGACATGAAG ACAGGGGAGG GTGGTCTCCT CCAAGTCACA
    CTGGAAGGCA TCAACCTCAA ATTTATGCAC AGCCAGTTCC TGAAACTAAA GAAGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301570.1
    CDS432..5231
    Translation

    Target ORF information:

    RefSeq Version XM_009303295.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X36, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303295.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GTATTTGGGC TAAATTCTCT AATGGAGATT
    GTAACAGAGA TCGGCCCGGG GAGCGGAGAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGAATTGTGG AGCAAGCACC AGGATAACAG AAAGCAAAAA
    GCTTTGGAAA AAGAACAGAG TGGTTTAAAT AAACGGAAAT CAGGAGCGGA TGGAGTAAAA
    CAACGTGTAG AAGGAGGAGA CACTGAGGAG AAGAAATCAC AGATCAGTGC AGACAGTGGC
    CTCAGTGTGA CATCTGGCTC ACAGAAGAGT GATACGGATT CCCTGGCGAG CTCTGAACCT
    CCTGCTCTGA CCAGAAGCAC CAGCCAGGAC TCAGAGGCCA GCACAGTAAG TAACAGCTCA
    GGTGAGACGC TCGGTGCAGA CAGTGACCTG AGCAGCACTG CTGGAGATGG CCTGACAGGA
    AGACATGGTC AGCATCTCAA CCTGTCCCGT GGCACACTGT CTGACAGTGA GATTGAGACC
    AACCCTGCAA CCAGCTCAGT GTTTGGCAAG ACACACAAGC TGAAGCCAGG AGTGAAGGAG
    CCTGTTGGTG TTAATAAAGC AGCACCTGCT CCACCCGTTG AGGATGTTAG CATGAGGATC
    TATCTGTGTG AGGGGCTTCT GGGCCGGGAT AAAAGCTCAG TTTGGGACCA GTTGGAGGAT
    GCTGCAATGG AAACCTTTTC TCTGAGTAAA GAGCGCTCAA CTCTGTGGGA TCAGATGCAG
    TTCTGGGAGG ACGCCTTCCT GGATGCCGTA ATGCTGGAAC GGGAAGGAAT GGGAATGGAT
    CAGGGACCAC AAGAAATGAT TGACAGGTAT CTTTCTCTGG GAGATCATGA CCGCAAGAGA
    CTGGAGGATG ATGAGGACAG ACTGCTGGCC ACGCTTCTGC ACAATATGAT TGCTTACATG
    CTTATGATGA AGGTCGGTAA AAACGACATC AGGAAGAAAG TACGTCGTCT TATGGGCAAA
    TCCCACATTG GCCTCACTCA CAGTCAGGAG ATCAATGAAG TCCTGGATCG CCTCGCACAT
    TTAAGCGGTC GGGAGCTTTC TATCAGGCCG AGTGGCAGCA GGCATATTAA AAAACAGACA
    TTTGTTGTGC ATGCTGGGAC AGATACAACT GGTGACATAT TCTTCATGGA GGTGTGTGAT
    GATTGCATCG TCTTGCGCAG TAATATTGGC ACAGTCTATG AGCGCTGGTG GTATGAGAAG
    CTCATAAATA TGACTTACTG TCCCAAAACT AAAGTGTTGT GTCTGTGGAG GAGAAACGGC
    CAGGAGACGC AGCTAAACAA GTTTTACACT AAGAAGTGTC GGGAATTGTA CTACTGTGTT
    AAAGACAGCA TGGAGCGTGC TGCTGCTCGA CAACAAAGCA TCAAACCAGG TCCAGAGCTT
    GGAGGCGAGT TTCCGGTTCA GGACATGAAG ACAGGGGAGG GTGGTCTCCT CCAAGTCACA
    CTGGAAGGCA TCAACCTCAA ATTTATGCAC AGCCAGTTCC TGAAACTAAA GAAGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48942
    Clone ID Related Accession (Same CDS sequence) XM_009303296.3 , XM_009303296.2
    Accession Version XM_009303296.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4743bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X36
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303296.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGAATTGTGG AGCAAGCACC AGGATAACAG AAAGCAAAAA
    GCTTTGGAAA AAGAACAGAG TGGTTTAAAT AAACGGAAAT CAGGAGCGGA TGGAGTAAAA
    CAACGTGTAG AAGGAGGAGA CACTGAGGAG AAGAAATCAC AGATCAGTGC AGACAGTGGC
    CTCAGTGTGA CATCTGGCTC ACAGAAGAGT GATACGGATT CCCTGGCGAG CTCTGAACCT
    CCTGCTCTGA CCAGAAGCAC CAGCCAGGAC TCAGAGGCCA GCACAGTGGT AAGTAACAGC
    TCAGGTGAGA CGCTCGGTGC AGACAGTGAC CTGAGCAGCA CTGCTGGAGA TGGCCTGACA
    GGAAGACATG GTCAGCATCT CAACCTGTCC CGTGGCACAC TGTCTGACAG TGAGATTGAG
    ACCAACCCTG CAACCAGCTC AGTGTTTGGC AAGACACACA AGCTGAAGCC AGGAGTGAAG
    GAGCCTGTTG GTGTTAATAA AGCAGCACCT GCTCCACCCG TTGAGGATGT TAGCATGAGG
    ATCTATCTGT GTGAGGGGCT TCTGGGCCGG GATAAAAGCT CAGTTTGGGA CCAGTTGGAG
    GATGCTGCAA TGGAAACCTT TTCTCTGAGT AAAGAGCGCT CAACTCTGTG GGATCAGATG
    CAGTTCTGGG AGGACGCCTT CCTGGATGCC GTAATGCTGG AACGGGAAGG AATGGGAATG
    GATCAGGGAC CACAAGAAAT GATTGACAGG TATCTTTCTC TGGGAGATCA TGACCGCAAG
    AGACTGGAGG ATGATGAGGA CAGACTGCTG GCCACGCTTC TGCACAATAT GATTGCTTAC
    ATGCTTATGA TGAAGGTCGG TAAAAACGAC ATCAGGAAGA AAGTACGTCG TCTTATGGGC
    AAATCCCACA TTGGCCTCAC TCACAGTCAG GAGATCAATG AAGTCCTGGA TCGCCTCGCA
    CATTTAAGCG GTCGGGAGCT TTCTATCAGG CCGAGTGGCA GCAGGCATAT TAAAAAACAG
    ACATTTGTTG TGCATGCTGG GACAGATACA ACTGGTGACA TATTCTTCAT GGAGGTGTGT
    GATGATTGCA TCGTCTTGCG CAGTAATATT GGCACAGTCT ATGAGCGCTG GTGGTATGAG
    AAGCTCATAA ATATGACTTA CTGTCCCAAA ACTAAAGTGT TGTGTCTGTG GAGGAGAAAC
    GGCCAGGAGA CGCAGCTAAA CAAGTTTTAC ACTAAGAAGT GTCGGGAATT GTACTACTGT
    GTTAAAGACA GCATGGAGCG TGCTGCTGCT CGACAACAAA GCATCAAACC AGGTCCAGAG
    CTTGGAGGCG AGTTTCCGGT TCAGGACATG AAGACAGGGG AGGGTGGTCT CCTCCAAGTC
    ACACTGGAAG GCATCAACCT CAAATTTATG CACAGCCAGT TCCTGAAACT AAAGAAGTGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301571.1
    CDS432..5174
    Translation

    Target ORF information:

    RefSeq Version XM_009303296.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X37, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303296.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGAATTGTGG AGCAAGCACC AGGATAACAG AAAGCAAAAA
    GCTTTGGAAA AAGAACAGAG TGGTTTAAAT AAACGGAAAT CAGGAGCGGA TGGAGTAAAA
    CAACGTGTAG AAGGAGGAGA CACTGAGGAG AAGAAATCAC AGATCAGTGC AGACAGTGGC
    CTCAGTGTGA CATCTGGCTC ACAGAAGAGT GATACGGATT CCCTGGCGAG CTCTGAACCT
    CCTGCTCTGA CCAGAAGCAC CAGCCAGGAC TCAGAGGCCA GCACAGTGGT AAGTAACAGC
    TCAGGTGAGA CGCTCGGTGC AGACAGTGAC CTGAGCAGCA CTGCTGGAGA TGGCCTGACA
    GGAAGACATG GTCAGCATCT CAACCTGTCC CGTGGCACAC TGTCTGACAG TGAGATTGAG
    ACCAACCCTG CAACCAGCTC AGTGTTTGGC AAGACACACA AGCTGAAGCC AGGAGTGAAG
    GAGCCTGTTG GTGTTAATAA AGCAGCACCT GCTCCACCCG TTGAGGATGT TAGCATGAGG
    ATCTATCTGT GTGAGGGGCT TCTGGGCCGG GATAAAAGCT CAGTTTGGGA CCAGTTGGAG
    GATGCTGCAA TGGAAACCTT TTCTCTGAGT AAAGAGCGCT CAACTCTGTG GGATCAGATG
    CAGTTCTGGG AGGACGCCTT CCTGGATGCC GTAATGCTGG AACGGGAAGG AATGGGAATG
    GATCAGGGAC CACAAGAAAT GATTGACAGG TATCTTTCTC TGGGAGATCA TGACCGCAAG
    AGACTGGAGG ATGATGAGGA CAGACTGCTG GCCACGCTTC TGCACAATAT GATTGCTTAC
    ATGCTTATGA TGAAGGTCGG TAAAAACGAC ATCAGGAAGA AAGTACGTCG TCTTATGGGC
    AAATCCCACA TTGGCCTCAC TCACAGTCAG GAGATCAATG AAGTCCTGGA TCGCCTCGCA
    CATTTAAGCG GTCGGGAGCT TTCTATCAGG CCGAGTGGCA GCAGGCATAT TAAAAAACAG
    ACATTTGTTG TGCATGCTGG GACAGATACA ACTGGTGACA TATTCTTCAT GGAGGTGTGT
    GATGATTGCA TCGTCTTGCG CAGTAATATT GGCACAGTCT ATGAGCGCTG GTGGTATGAG
    AAGCTCATAA ATATGACTTA CTGTCCCAAA ACTAAAGTGT TGTGTCTGTG GAGGAGAAAC
    GGCCAGGAGA CGCAGCTAAA CAAGTTTTAC ACTAAGAAGT GTCGGGAATT GTACTACTGT
    GTTAAAGACA GCATGGAGCG TGCTGCTGCT CGACAACAAA GCATCAAACC AGGTCCAGAG
    CTTGGAGGCG AGTTTCCGGT TCAGGACATG AAGACAGGGG AGGGTGGTCT CCTCCAAGTC
    ACACTGGAAG GCATCAACCT CAAATTTATG CACAGCCAGT TCCTGAAACT AAAGAAGTGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48942
    Clone ID Related Accession (Same CDS sequence) XM_009303296.3 , XM_009303296.2
    Accession Version XM_009303296.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4743bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product MAP kinase-activating death domain protein isoform X33
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303296.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGAATTGTGG AGCAAGCACC AGGATAACAG AAAGCAAAAA
    GCTTTGGAAA AAGAACAGAG TGGTTTAAAT AAACGGAAAT CAGGAGCGGA TGGAGTAAAA
    CAACGTGTAG AAGGAGGAGA CACTGAGGAG AAGAAATCAC AGATCAGTGC AGACAGTGGC
    CTCAGTGTGA CATCTGGCTC ACAGAAGAGT GATACGGATT CCCTGGCGAG CTCTGAACCT
    CCTGCTCTGA CCAGAAGCAC CAGCCAGGAC TCAGAGGCCA GCACAGTGGT AAGTAACAGC
    TCAGGTGAGA CGCTCGGTGC AGACAGTGAC CTGAGCAGCA CTGCTGGAGA TGGCCTGACA
    GGAAGACATG GTCAGCATCT CAACCTGTCC CGTGGCACAC TGTCTGACAG TGAGATTGAG
    ACCAACCCTG CAACCAGCTC AGTGTTTGGC AAGACACACA AGCTGAAGCC AGGAGTGAAG
    GAGCCTGTTG GTGTTAATAA AGCAGCACCT GCTCCACCCG TTGAGGATGT TAGCATGAGG
    ATCTATCTGT GTGAGGGGCT TCTGGGCCGG GATAAAAGCT CAGTTTGGGA CCAGTTGGAG
    GATGCTGCAA TGGAAACCTT TTCTCTGAGT AAAGAGCGCT CAACTCTGTG GGATCAGATG
    CAGTTCTGGG AGGACGCCTT CCTGGATGCC GTAATGCTGG AACGGGAAGG AATGGGAATG
    GATCAGGGAC CACAAGAAAT GATTGACAGG TATCTTTCTC TGGGAGATCA TGACCGCAAG
    AGACTGGAGG ATGATGAGGA CAGACTGCTG GCCACGCTTC TGCACAATAT GATTGCTTAC
    ATGCTTATGA TGAAGGTCGG TAAAAACGAC ATCAGGAAGA AAGTACGTCG TCTTATGGGC
    AAATCCCACA TTGGCCTCAC TCACAGTCAG GAGATCAATG AAGTCCTGGA TCGCCTCGCA
    CATTTAAGCG GTCGGGAGCT TTCTATCAGG CCGAGTGGCA GCAGGCATAT TAAAAAACAG
    ACATTTGTTG TGCATGCTGG GACAGATACA ACTGGTGACA TATTCTTCAT GGAGGTGTGT
    GATGATTGCA TCGTCTTGCG CAGTAATATT GGCACAGTCT ATGAGCGCTG GTGGTATGAG
    AAGCTCATAA ATATGACTTA CTGTCCCAAA ACTAAAGTGT TGTGTCTGTG GAGGAGAAAC
    GGCCAGGAGA CGCAGCTAAA CAAGTTTTAC ACTAAGAAGT GTCGGGAATT GTACTACTGT
    GTTAAAGACA GCATGGAGCG TGCTGCTGCT CGACAACAAA GCATCAAACC AGGTCCAGAG
    CTTGGAGGCG AGTTTCCGGT TCAGGACATG AAGACAGGGG AGGGTGGTCT CCTCCAAGTC
    ACACTGGAAG GCATCAACCT CAAATTTATG CACAGCCAGT TCCTGAAACT AAAGAAGTGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301571.1
    CDS435..5177
    Translation

    Target ORF information:

    RefSeq Version XM_009303296.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X33, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303296.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGAATTGTGG AGCAAGCACC AGGATAACAG AAAGCAAAAA
    GCTTTGGAAA AAGAACAGAG TGGTTTAAAT AAACGGAAAT CAGGAGCGGA TGGAGTAAAA
    CAACGTGTAG AAGGAGGAGA CACTGAGGAG AAGAAATCAC AGATCAGTGC AGACAGTGGC
    CTCAGTGTGA CATCTGGCTC ACAGAAGAGT GATACGGATT CCCTGGCGAG CTCTGAACCT
    CCTGCTCTGA CCAGAAGCAC CAGCCAGGAC TCAGAGGCCA GCACAGTGGT AAGTAACAGC
    TCAGGTGAGA CGCTCGGTGC AGACAGTGAC CTGAGCAGCA CTGCTGGAGA TGGCCTGACA
    GGAAGACATG GTCAGCATCT CAACCTGTCC CGTGGCACAC TGTCTGACAG TGAGATTGAG
    ACCAACCCTG CAACCAGCTC AGTGTTTGGC AAGACACACA AGCTGAAGCC AGGAGTGAAG
    GAGCCTGTTG GTGTTAATAA AGCAGCACCT GCTCCACCCG TTGAGGATGT TAGCATGAGG
    ATCTATCTGT GTGAGGGGCT TCTGGGCCGG GATAAAAGCT CAGTTTGGGA CCAGTTGGAG
    GATGCTGCAA TGGAAACCTT TTCTCTGAGT AAAGAGCGCT CAACTCTGTG GGATCAGATG
    CAGTTCTGGG AGGACGCCTT CCTGGATGCC GTAATGCTGG AACGGGAAGG AATGGGAATG
    GATCAGGGAC CACAAGAAAT GATTGACAGG TATCTTTCTC TGGGAGATCA TGACCGCAAG
    AGACTGGAGG ATGATGAGGA CAGACTGCTG GCCACGCTTC TGCACAATAT GATTGCTTAC
    ATGCTTATGA TGAAGGTCGG TAAAAACGAC ATCAGGAAGA AAGTACGTCG TCTTATGGGC
    AAATCCCACA TTGGCCTCAC TCACAGTCAG GAGATCAATG AAGTCCTGGA TCGCCTCGCA
    CATTTAAGCG GTCGGGAGCT TTCTATCAGG CCGAGTGGCA GCAGGCATAT TAAAAAACAG
    ACATTTGTTG TGCATGCTGG GACAGATACA ACTGGTGACA TATTCTTCAT GGAGGTGTGT
    GATGATTGCA TCGTCTTGCG CAGTAATATT GGCACAGTCT ATGAGCGCTG GTGGTATGAG
    AAGCTCATAA ATATGACTTA CTGTCCCAAA ACTAAAGTGT TGTGTCTGTG GAGGAGAAAC
    GGCCAGGAGA CGCAGCTAAA CAAGTTTTAC ACTAAGAAGT GTCGGGAATT GTACTACTGT
    GTTAAAGACA GCATGGAGCG TGCTGCTGCT CGACAACAAA GCATCAAACC AGGTCCAGAG
    CTTGGAGGCG AGTTTCCGGT TCAGGACATG AAGACAGGGG AGGGTGGTCT CCTCCAAGTC
    ACACTGGAAG GCATCAACCT CAAATTTATG CACAGCCAGT TCCTGAAACT AAAGAAGTGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48943
    Clone ID Related Accession (Same CDS sequence) XM_009303297.3 , XM_009303297.2
    Accession Version XM_009303297.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4869bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product MAP kinase-activating death domain protein isoform X37
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303297.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGGAGCGGAT GGAGTAAAAC AACGTGTAGA AGGAGGAGAC
    ACTGAGGAGA AGAAATCACA GATCAGTGCA GACAGTGGCC TCAGTGTGAC ATCTGGCTCA
    CAGAAGAGTG ATACGGATTC CCTGGCGAGC TCTGAACCTC CTGCTCTGAC CAGAAGCACC
    AGCCAGGACT CAGAGGCCAG CACAGTGGTA AGTAACAGCT CAGGTGAGAC GCTCGGTGCA
    GACAGTGACC TGAGCAGCAC TGCTGGAGAT GGCCTGACAG GAAGACATGG TCAGCATCTC
    AACCTGTCCC GTGGCACACT GTCTGACAGT GAGATTGAGA CCAACCCTGC AACCAGCTCA
    GTGTTTGGCA AGACACACAA GCTGAAGCCA GGAGTGAAGG AGCCTGTTGG TGTTAATAAA
    GCAGCACCTG CTCCACCCGT TGAGGATGTT AGCATGAGGA TCTATCTGTG TGAGGGGCTT
    CTGGGCCGGG ATAAAAGCTC AGTTTGGGAC CAGTTGGAGG ATGCTGCAAT GGAAACCTTT
    TCTCTGAGTA AAGAGCGCTC AACTCTGTGG GATCAGATGC AGTTCTGGGA GGACGCCTTC
    CTGGATGCCG TAATGCTGGA ACGGGAAGGA ATGGGAATGG ATCAGGGACC ACAAGAAATG
    ATTGACAGGT ATCTTTCTCT GGGAGATCAT GACCGCAAGA GACTGGAGGA TGATGAGGAC
    AGACTGCTGG CCACGCTTCT GCACAATATG ATTGCTTACA TGCTTATGAT GAAGGTCGGT
    AAAAACGACA TCAGGAAGAA AGTACGTCGT CTTATGGGCA AATCCCACAT TGGCCTCACT
    CACAGTCAGG AGATCAATGA AGTCCTGGAT CGCCTCGCAC ATTTAAGCGG TCGGGAGCTT
    TCTATCAGGC CGAGTGGCAG CAGGCATATT AAAAAACAGA CATTTGTTGT GCATGCTGGG
    ACAGATACAA CTGGTGACAT ATTCTTCATG GAGGTGTGTG ATGATTGCAT CGTCTTGCGC
    AGTAATATTG GCACAGTCTA TGAGCGCTGG TGGTATGAGA AGCTCATAAA TATGACTTAC
    TGTCCCAAAA CTAAAGTGTT GTGTCTGTGG AGGAGAAACG GCCAGGAGAC GCAGCTAAAC
    AAGTTTTACA CTAAGAAGTG TCGGGAATTG TACTACTGTG TTAAAGACAG CATGGAGCGT
    GCTGCTGCTC GACAACAAAG CATCAAACCA GGTCCAGAGC TTGGAGGCGA GTTTCCGGTT
    CAGGACATGA AGACAGGGGA GGGTGGTCTC CTCCAAGTCA CACTGGAAGG CATCAACCTC
    AAATTTATGC ACAGCCAGGT TTTCATAGAG CTGAATCACA TTAAAAAGTG CAATACAGTG
    AAAGGAGTCT TTGTCCTGGA GGAGTTTGTT CCTGAAACTA AAGAAGTGGT GATCCATAAG
    TACAAGACTC CTATGGCACA TCAGATCTGT TACTCGGTGC TCTGCCTCTT CTCCTACATG
    GCGGCGGTCA AGGGGAAAGA GTCAGAGGGC AAACCCAAGA TGCTGTCCCC TCGTCCACTA
    GCGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301572.1
    CDS431..5299
    Translation

    Target ORF information:

    RefSeq Version XM_009303297.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X38, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303297.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGGAGCGGAT GGAGTAAAAC AACGTGTAGA AGGAGGAGAC
    ACTGAGGAGA AGAAATCACA GATCAGTGCA GACAGTGGCC TCAGTGTGAC ATCTGGCTCA
    CAGAAGAGTG ATACGGATTC CCTGGCGAGC TCTGAACCTC CTGCTCTGAC CAGAAGCACC
    AGCCAGGACT CAGAGGCCAG CACAGTGGTA AGTAACAGCT CAGGTGAGAC GCTCGGTGCA
    GACAGTGACC TGAGCAGCAC TGCTGGAGAT GGCCTGACAG GAAGACATGG TCAGCATCTC
    AACCTGTCCC GTGGCACACT GTCTGACAGT GAGATTGAGA CCAACCCTGC AACCAGCTCA
    GTGTTTGGCA AGACACACAA GCTGAAGCCA GGAGTGAAGG AGCCTGTTGG TGTTAATAAA
    GCAGCACCTG CTCCACCCGT TGAGGATGTT AGCATGAGGA TCTATCTGTG TGAGGGGCTT
    CTGGGCCGGG ATAAAAGCTC AGTTTGGGAC CAGTTGGAGG ATGCTGCAAT GGAAACCTTT
    TCTCTGAGTA AAGAGCGCTC AACTCTGTGG GATCAGATGC AGTTCTGGGA GGACGCCTTC
    CTGGATGCCG TAATGCTGGA ACGGGAAGGA ATGGGAATGG ATCAGGGACC ACAAGAAATG
    ATTGACAGGT ATCTTTCTCT GGGAGATCAT GACCGCAAGA GACTGGAGGA TGATGAGGAC
    AGACTGCTGG CCACGCTTCT GCACAATATG ATTGCTTACA TGCTTATGAT GAAGGTCGGT
    AAAAACGACA TCAGGAAGAA AGTACGTCGT CTTATGGGCA AATCCCACAT TGGCCTCACT
    CACAGTCAGG AGATCAATGA AGTCCTGGAT CGCCTCGCAC ATTTAAGCGG TCGGGAGCTT
    TCTATCAGGC CGAGTGGCAG CAGGCATATT AAAAAACAGA CATTTGTTGT GCATGCTGGG
    ACAGATACAA CTGGTGACAT ATTCTTCATG GAGGTGTGTG ATGATTGCAT CGTCTTGCGC
    AGTAATATTG GCACAGTCTA TGAGCGCTGG TGGTATGAGA AGCTCATAAA TATGACTTAC
    TGTCCCAAAA CTAAAGTGTT GTGTCTGTGG AGGAGAAACG GCCAGGAGAC GCAGCTAAAC
    AAGTTTTACA CTAAGAAGTG TCGGGAATTG TACTACTGTG TTAAAGACAG CATGGAGCGT
    GCTGCTGCTC GACAACAAAG CATCAAACCA GGTCCAGAGC TTGGAGGCGA GTTTCCGGTT
    CAGGACATGA AGACAGGGGA GGGTGGTCTC CTCCAAGTCA CACTGGAAGG CATCAACCTC
    AAATTTATGC ACAGCCAGGT TTTCATAGAG CTGAATCACA TTAAAAAGTG CAATACAGTG
    AAAGGAGTCT TTGTCCTGGA GGAGTTTGTT CCTGAAACTA AAGAAGTGGT GATCCATAAG
    TACAAGACTC CTATGGCACA TCAGATCTGT TACTCGGTGC TCTGCCTCTT CTCCTACATG
    GCGGCGGTCA AGGGGAAAGA GTCAGAGGGC AAACCCAAGA TGCTGTCCCC TCGTCCACTA
    GCGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48943
    Clone ID Related Accession (Same CDS sequence) XM_009303297.3 , XM_009303297.2
    Accession Version XM_009303297.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4869bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product MAP kinase-activating death domain protein isoform X34
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303297.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGGAGCGGAT GGAGTAAAAC AACGTGTAGA AGGAGGAGAC
    ACTGAGGAGA AGAAATCACA GATCAGTGCA GACAGTGGCC TCAGTGTGAC ATCTGGCTCA
    CAGAAGAGTG ATACGGATTC CCTGGCGAGC TCTGAACCTC CTGCTCTGAC CAGAAGCACC
    AGCCAGGACT CAGAGGCCAG CACAGTGGTA AGTAACAGCT CAGGTGAGAC GCTCGGTGCA
    GACAGTGACC TGAGCAGCAC TGCTGGAGAT GGCCTGACAG GAAGACATGG TCAGCATCTC
    AACCTGTCCC GTGGCACACT GTCTGACAGT GAGATTGAGA CCAACCCTGC AACCAGCTCA
    GTGTTTGGCA AGACACACAA GCTGAAGCCA GGAGTGAAGG AGCCTGTTGG TGTTAATAAA
    GCAGCACCTG CTCCACCCGT TGAGGATGTT AGCATGAGGA TCTATCTGTG TGAGGGGCTT
    CTGGGCCGGG ATAAAAGCTC AGTTTGGGAC CAGTTGGAGG ATGCTGCAAT GGAAACCTTT
    TCTCTGAGTA AAGAGCGCTC AACTCTGTGG GATCAGATGC AGTTCTGGGA GGACGCCTTC
    CTGGATGCCG TAATGCTGGA ACGGGAAGGA ATGGGAATGG ATCAGGGACC ACAAGAAATG
    ATTGACAGGT ATCTTTCTCT GGGAGATCAT GACCGCAAGA GACTGGAGGA TGATGAGGAC
    AGACTGCTGG CCACGCTTCT GCACAATATG ATTGCTTACA TGCTTATGAT GAAGGTCGGT
    AAAAACGACA TCAGGAAGAA AGTACGTCGT CTTATGGGCA AATCCCACAT TGGCCTCACT
    CACAGTCAGG AGATCAATGA AGTCCTGGAT CGCCTCGCAC ATTTAAGCGG TCGGGAGCTT
    TCTATCAGGC CGAGTGGCAG CAGGCATATT AAAAAACAGA CATTTGTTGT GCATGCTGGG
    ACAGATACAA CTGGTGACAT ATTCTTCATG GAGGTGTGTG ATGATTGCAT CGTCTTGCGC
    AGTAATATTG GCACAGTCTA TGAGCGCTGG TGGTATGAGA AGCTCATAAA TATGACTTAC
    TGTCCCAAAA CTAAAGTGTT GTGTCTGTGG AGGAGAAACG GCCAGGAGAC GCAGCTAAAC
    AAGTTTTACA CTAAGAAGTG TCGGGAATTG TACTACTGTG TTAAAGACAG CATGGAGCGT
    GCTGCTGCTC GACAACAAAG CATCAAACCA GGTCCAGAGC TTGGAGGCGA GTTTCCGGTT
    CAGGACATGA AGACAGGGGA GGGTGGTCTC CTCCAAGTCA CACTGGAAGG CATCAACCTC
    AAATTTATGC ACAGCCAGGT TTTCATAGAG CTGAATCACA TTAAAAAGTG CAATACAGTG
    AAAGGAGTCT TTGTCCTGGA GGAGTTTGTT CCTGAAACTA AAGAAGTGGT GATCCATAAG
    TACAAGACTC CTATGGCACA TCAGATCTGT TACTCGGTGC TCTGCCTCTT CTCCTACATG
    GCGGCGGTCA AGGGGAAAGA GTCAGAGGGC AAACCCAAGA TGCTGTCCCC TCGTCCACTA
    GCGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301572.1
    CDS434..5302
    Translation

    Target ORF information:

    RefSeq Version XM_009303297.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio MAP-kinase activating death domain (madd), transcript variant X34, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303297.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGAGAAAA AGAAAATGTG TCCCCGCCTC CTGGACTACC TGGTGGTAGT TGGAGCCAGG 
    CAACCCAGCA ATGACAGTGT GGCACAGACA CCTCAGCTGT TGCGGCGTTA TCCGCTGGAG
    GACCACAATG ACTTTCCTCT GCCACCTGAT GTGGTCTTCT TCTGCCAGCC TGAGGGCTGT
    CTGAGCATCC GTCAGCGGCG GGTCAGTCTC CGCGATGACA CCTCTTTCGT CTTCACTCTC
    ACTGACAAAG ACTCGGGCAT CACTCGCTAT GGAATCTGTC TCAACTTCTA CCGCTCTTTT
    CAAAAGGCCC ACCATCGCCC AAGAGCTGAA GGAAAAGGAG AAAAGCCTGC TCATACAGAC
    ACTGCAGTGG AGGCCACAGA GAAATCTGAC CCCTCCACTT TGACTCTTTC AGGGGAACAT
    TCGGCGCCCC CTGCAGGAGA TGGGACTTTG CTGCCAGGCG AGCCGGGTAG CAGCGGGAAG
    TCTCCGCGAT CTAAACGCAG TGGCCGAATA GCACCACAGA ACCGCAACAG CATGTTAACA
    TCTCTGTGTA TTCTGAGCCA CTACCCTTTC TTCTCAACAT TCAGAGAGTG TCTGTACATC
    CTCAAGCGCA TGGTGGACTG CTGTAGTCAG CGGCTCAACC AGAGACCCGG AGCTCCCAAA
    TCCACACAAC GGGACACGAT GTGGCGTGTG TTCACGGGAG CTCTTTCTGT GGAAGAAAAG
    GAGAAGGGCA GTCAGGTGCT CCAGGACCTG AGGGAGATCG AGTCATGGGT TTATCGGCTG
    CTGCGCTCAC CGGTGCCTGT TGCAGGTCAG CGGCGTGTGG ATGTGGAGGT CCTGCCCCAT
    GAGCTGCAGC CTGCTCTGAC ATTTGCCCTT CCAGACCCGT CCCGCTTCAG CATCGTAGAC
    TTCCCGCTCC ACCTGCCACT GGAGCTGCTG GGAGTCGATG CCTGTCTGCA GGTCTTGGCC
    TGCATTCTGC TGGAGCACAA GGTGGTACTG CAGTCTCGAG ATTATAACGC TCTCTCAATG
    AGCGTCATGG CGTTTGTTTC GATGATTTAC CCTCTTGAGT ACATGTTTCC TGTCATTCCA
    CTGTTGCCCA CCTGCATGGC CTCTGCAGAA CAGCTCCTAT TAGCACCCAC TCCATACGTC
    ATTGGTGTGC CAGCCAGTTT CTTCTTGTAC AAGTGTGATT TTAAGATGCC TGATGATGTT
    TGGTTAGTGG ATCTGGACTG CAACAAGGTT ATTGTTCCAA GCAATGCAGA ACTGCTCCCA
    CCCTTACCTG AACCTGAGGC CTCTGAGCTG AAGAAACACT TGAAGCAGGC TCTGGCTAGC
    ATGAGTCTAA ACACACAGCC TATTCTGAAC CTGGAGAAGT TTCAGGATGG ACAGGAGTTG
    TCTTTATTGC CCCCCGGTCG TGACAAAGCT TCCCCGTCCT CCACTGAGTT CAACCCACTC
    ATCTACGGCA ATGATGTGGA CTCAGTTGAT GTTGCCACAA GGGTGGCAAT GGTGAGGTTC
    TTCAACTCTC CAAACGTGCT GCAAGGGTTC CAGATGCACA CACGCACTCT CCGTCTCTTC
    CCTCGTCCTG TGGTGGCCTT TCAGTCCACA TCTTTCCTGG CTTCTAGGCC ACGACGAAAT
    GGATTCACAG AAAAGCTCTC CCACACTCAG GCAGTGGAGT ACTATGGAGA ATGGGCTCTC
    AACCCCACCA ACCTGGCTTT CCAAAGGATT CATAACAATG TGTATGACCC CTCTCTAATA
    GGCGATAAGC CTAAGTGGTA TGCCCACCAG TTACAGCCAG TATTCTACCG CGTGTATGAT
    GGAAACTCTC ATTTAGCGGA GGCCTTGAGT GGACCCCTGC AGGATGAGAC CAATGATTCA
    GACCCAACAG ACGACAGTGG CAGCGACAGT GAAGCATATG ATGACTCCAG CTCCTCCTAC
    TCATCTCTTG GAGATTTTGT GAATGAGATG ATCAAAGGGG ACATACAGGG AGACACACCA
    AATGTTGATC CCCTGACCCA TGCTGCACTG GGAGATGCAG ATGAGGTTGA GATCCATGAC
    TTTCACGAGT ATAAGGGGAA CAGCGGTGAA CCAGAGCCAG AAGGCCCCAC AGAAGCAGCG
    GACAGTCAGC CCTTGCGGTC CAGCTCTAGC ACCACAGCCA GTTCCAGCCC CAGTACAGTC
    ATACAGGGAG TCAACCATGA GCAGAAGGAA CCTGTTGAGG TGGAGCCCAT AGCAAATGTG
    ACTTACCCAA GCTCTGCCCC AGTATTGGGT CCTCCTCCTT TTACAAGACC CACCCCTGAT
    CCAACCCTTG TTGACCCTGC CAATAAGAAG CGAGAATATG ACAACCCCTA TTTTGAGCCC
    CAGTACGGTT TCCCTACAGA GGAAGATGCT GAGACTGATG AGCAGGAGGA GAGTTACACA
    CCACGATTTA ACCAGAATCT CAATGGCAAT AAGCCTTCTC GTCCACTGAG ACCCAGCAGC
    CTGAAGCTTC CGGGTGAATC TGACGGAGAG GGAGACTCCA GAAATAGCTC TCCTAACTCA
    ACCATCTCCA ACAATAGCAG TGATGGCTTT GGGGGACTCA TGTCCTTTGC CAGTAACTTG
    TATAAAAACC ATGGCACCAG TTTCAGTCTG TCCAGCCTGG CTCTGCCTAA TAAAGCGAGA
    GAGAAGAACA CGCCGTTTCC CAGCCTCAAA GGTGCCCGAG CCCCCAGAGC ACTGGTAGAT
    CAGAAATCCT CTGTTATTAA GCACAGCCCT ACTGTGAAGA GAGAGTCTCC GTCTCCTCAA
    GGCAGAGCCA ACAATACCAG TGAGAACCAG CAGTTTCTAA AGGAGGTGGT CCAGAGCGTG
    CTGGAGGGGC AGGGTGTGGG CTGGCTTAAC ATGAAGAAGG TGCGTCGCCT GCTAGAGAAT
    GAACAGCTCA GAGTCTTTGT GCTCAGCAAA CTCAACCGTG CGGTCCAGTC AGAGGAGGAC
    GCCCAGCAGG AAGTTATAAG AGATGTGGAG ATAAATAGAA AGGTCTACAA AGGCATGCTG
    GATATTTTGA AGTGCACGGT GTCCAGTTTG GAGCATTCAT ACACTAATGC TGGCTTAGGG
    GGCATGGCCA GTGTCTTCAG TCTGCTAGAG ATAGCACGCA CCCACTACCA AACCAAAGAT
    CCAGAGAAGC GGAAACGGAG CCCCACCGAA GGGCTTAGCA GTCCAGGCAG TAAGGAGAGC
    CCATCGGGCC GAATGGAGAG TGCCAGGGCG GCAGGGGTGC TTCTGGTACC CCGTATCCAA
    TTGCCGCCAC CCTCCTCTGG GAAATCATCA CGCCAGTTTG ATACCCGGAG TCTGAACGAG
    GAGAATTTTA TTGCCTCCAT TGGAGCGGAT GGAGTAAAAC AACGTGTAGA AGGAGGAGAC
    ACTGAGGAGA AGAAATCACA GATCAGTGCA GACAGTGGCC TCAGTGTGAC ATCTGGCTCA
    CAGAAGAGTG ATACGGATTC CCTGGCGAGC TCTGAACCTC CTGCTCTGAC CAGAAGCACC
    AGCCAGGACT CAGAGGCCAG CACAGTGGTA AGTAACAGCT CAGGTGAGAC GCTCGGTGCA
    GACAGTGACC TGAGCAGCAC TGCTGGAGAT GGCCTGACAG GAAGACATGG TCAGCATCTC
    AACCTGTCCC GTGGCACACT GTCTGACAGT GAGATTGAGA CCAACCCTGC AACCAGCTCA
    GTGTTTGGCA AGACACACAA GCTGAAGCCA GGAGTGAAGG AGCCTGTTGG TGTTAATAAA
    GCAGCACCTG CTCCACCCGT TGAGGATGTT AGCATGAGGA TCTATCTGTG TGAGGGGCTT
    CTGGGCCGGG ATAAAAGCTC AGTTTGGGAC CAGTTGGAGG ATGCTGCAAT GGAAACCTTT
    TCTCTGAGTA AAGAGCGCTC AACTCTGTGG GATCAGATGC AGTTCTGGGA GGACGCCTTC
    CTGGATGCCG TAATGCTGGA ACGGGAAGGA ATGGGAATGG ATCAGGGACC ACAAGAAATG
    ATTGACAGGT ATCTTTCTCT GGGAGATCAT GACCGCAAGA GACTGGAGGA TGATGAGGAC
    AGACTGCTGG CCACGCTTCT GCACAATATG ATTGCTTACA TGCTTATGAT GAAGGTCGGT
    AAAAACGACA TCAGGAAGAA AGTACGTCGT CTTATGGGCA AATCCCACAT TGGCCTCACT
    CACAGTCAGG AGATCAATGA AGTCCTGGAT CGCCTCGCAC ATTTAAGCGG TCGGGAGCTT
    TCTATCAGGC CGAGTGGCAG CAGGCATATT AAAAAACAGA CATTTGTTGT GCATGCTGGG
    ACAGATACAA CTGGTGACAT ATTCTTCATG GAGGTGTGTG ATGATTGCAT CGTCTTGCGC
    AGTAATATTG GCACAGTCTA TGAGCGCTGG TGGTATGAGA AGCTCATAAA TATGACTTAC
    TGTCCCAAAA CTAAAGTGTT GTGTCTGTGG AGGAGAAACG GCCAGGAGAC GCAGCTAAAC
    AAGTTTTACA CTAAGAAGTG TCGGGAATTG TACTACTGTG TTAAAGACAG CATGGAGCGT
    GCTGCTGCTC GACAACAAAG CATCAAACCA GGTCCAGAGC TTGGAGGCGA GTTTCCGGTT
    CAGGACATGA AGACAGGGGA GGGTGGTCTC CTCCAAGTCA CACTGGAAGG CATCAACCTC
    AAATTTATGC ACAGCCAGGT TTTCATAGAG CTGAATCACA TTAAAAAGTG CAATACAGTG
    AAAGGAGTCT TTGTCCTGGA GGAGTTTGTT CCTGAAACTA AAGAAGTGGT GATCCATAAG
    TACAAGACTC CTATGGCACA TCAGATCTGT TACTCGGTGC TCTGCCTCTT CTCCTACATG
    GCGGCGGTCA AGGGGAAAGA GTCAGAGGGC AAACCCAAGA TGCTGTCCCC TCGTCCACTA
    GCGAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.