met cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	met
Entrez Gene ID	100150664
Full Name	MET proto-oncogene, receptor tyrosine kinase
Synonyms	c-met,cmet
General protein information	Preferred Names MET proto-oncogene, receptor tyrosine kinase Names hepatocyte growth factor receptor met proto-oncogene (hepatocyte growth factor receptor)
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005162994.3	XP_005163051.1	hepatocyte growth factor receptor isoform X1
XM_005162994.4	XP_005163051.1	hepatocyte growth factor receptor isoform X1
XM_005162995.4	XP_005163052.1	hepatocyte growth factor receptor isoform X2
XM_005162995.3	XP_005163052.1	hepatocyte growth factor receptor isoform X2
NM_001007124.1	NP_001007125.1	hepatocyte growth factor receptor precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MET	NP_001002963.1
Rattus norvegicus (Norway rat)	Met	NP_113705.1
Gallus gallus (chicken)	MET	NP_990543.1
Homo sapiens (human)	MET	NP_001120972.1
Pan troglodytes (chimpanzee)	MET	NP_001129302.1
Bos taurus (cattle)	MET	NP_001013017.2
Mus musculus (house mouse)	Met	NP_032617.2
Danio rerio (zebrafish)	met	NP_001007125.1
Xenopus tropicalis (tropical clawed frog)	met	XP_002935001.1
	MET	NP_001162100.1

Gene Ontology
Bibliography

Related articles in PubMed

Maternal and embryonic exposure to the water soluble fraction of crude oil or lead induces behavioral abnormalities in zebrafish (Danio rerio), and the mechanisms involved.
Wang Y, Shen C, Wang C, Zhou Y, Gao D, Zuo Z
Chemosphere1917-16(2018 Jan)

Anti-angiogenic activity of water extract from Euphorbia pekinensis Rupr.
Zhang W, Liu B, Feng Y, Liu J, Ma Z, Zheng J, Xia Q, Ni Y, Li F, Lin R
Journal of ethnopharmacology206337-346(2017 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results demonstrate a novel role of Klf8, achieved via modulation of p53 and met expression, in the maintenance neuronal progenitors and development of Purkinje cells and the proliferation of granule cells in the cerebella of zebrafish embryos
Title: Modulation of p53 and met expression by Kr?ppel-like factor 8 regulates zebrafish cerebellar development.

Altogether, these results reveal a novel and essential role for HGF signaling in the intrapancreatic ducts during exocrine morphogenesis.
Title: Hepatocyte growth factor signaling in intrapancreatic ductal cells drives pancreatic morphogenesis.

GrnA positively modulates hepatic MET expression both in vivo and in vitro.
Title: Progranulin A-mediated MET signaling is essential for liver morphogenesis in zebrafish.

Results describe roles for Met signaling in coordinating growth and cell type specification within the developing cerebellum, and in migration of hindbrain neurons.
Title: The autism susceptibility gene met regulates zebrafish cerebellar development and facial motor neuron migration.

suggest a role for Met during endoderm specification and indicate that mechanisms of liver development are conserved between mammals and bony fish
Title: Hgf/c-met expression and functional analysis during zebrafish embryogenesis.

The following met gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the met cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa01347	NM_001007124.1 Latest version!	Danio rerio MET proto-oncogene, receptor tyrosine kinase (met), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa29330	XM_005162994.4 Latest version!	Danio rerio MET proto-oncogene, receptor tyrosine kinase (met), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa29331	XM_005162995.4 Latest version!	Danio rerio MET proto-oncogene, receptor tyrosine kinase (met), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
ODa29330	XM_005162994.3	Danio rerio MET proto-oncogene, receptor tyrosine kinase (met), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa29331	XM_005162995.3	Danio rerio MET proto-oncogene, receptor tyrosine kinase (met), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

met ( NM_001007124.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001007125.1 Danio rerio MET proto-oncogene, receptor tyrosine kinase (met), mRNA.

Price & Availability↑

CloneID	ODa01347
Clone ID Related Accession (Same CDS sequence)	NM_001007124.1
Accession Version	NM_001007124.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Danio rerio(zebrafish)
Product	hepatocyte growth factor receptor precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY687384.1. On Jan 29, 2011 this sequence version replaced XM_001922642.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY687384.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGACAATTC ACTATTCTGA AGCTGCTTCC ATCCTAATCA TCCTTCAGTC GCTGTGGTGG 
GGCTTGAATT GTCAATGTGA GGAACCAATA GAAAGCTCCA AACTCGACCT CTCAGTGACC 
TATGACCTCC CTTACTTTGT GTCTGACACC CCCATTCAGA AGCTGTTGGA AATCAATGGA 
ACAGTGTATG TCGGTGCCGT CAATAGACTT TACGCTCTGT CGAAAGACCT GAAGAAGAAA 
CATGAGTATA AGACTGGACC GGTCCACGAG GGTCCAGACT GCAAGACCCC AACAGATCAA 
TGCAGTGGTT GCGAAAACAA GCCCCGTAAC ATAAACAACA CCAATATGGC CCTGTTAATG 
GAGACGTTCT ATGACCTGGA ACTCTTCAGC TGTGGCTCAG TCGGAAATGG CGTCTGCAGT 
CGTCATGTGT TAGAGGATGG GCCTCTGGGT GCGGAAGTAA CTTGCATGTA CACCAAAAAG 
AATGAAGGCA GCAGCCACGG ATGCCCAGAC TGCCTGGCTG GACCTGCGGG CACTCAGATC 
CTCAACATAA TGAGCGGTCG TGTTGTGAGG TTCTTCGTTG CGAACTCTGA ACCTCTTGAG 
TCAAACGGTC CACGTCTCCA CCACACTATT TCCATTAGGA AGATGCGTGA AACTCAAGAT 
GGCTTTGAGT TCTTTTCCGA GCAGTCCTAC ATGGATTTGG CCCCTTCACT GCGGGGGAAC 
TATCCACTAC ATTATGTCTA CTCTTTCCAG AGTGGTCCTT ATGTATATTT TCTCACCGTC 
CAACGCGAAG GTGGCAACTC GAAAGCTTTC CACACGAGAA TCGTACGCAT GTGTTCTTCA 
GATTCTGAGA TCCTGCGTTA TGTAGAAATG CCCTTTGAGT GCATTTACTC TGAGCGAAGG 
AGAAAAAAGC GTTCGGCTCA AGTGGTTTTC AACGTTCTCC AGGCTGCTCA TGTGGCCAAA 
GTTGGCTATG ACTTTCAGCA GGAGATGGGC TTGAAAGAAG GAGAGGACGT GCTGTTTGCT 
GCCTTTGCCC GGAGCAAACC GGACTCACCA GAGCCCACCG CCAGCTCCGC CGTTTGCCTT 
ATCTCCATCA CGGACATCAA TGAATTCTTC AAGGTTTTCA TTCAGAAGGG TTACACAAGG 
AAACTCCATC ACTTTCCAGG ATCTGAAGAG AAAACCTTCA ACCAGACGTT TGTTGGAGAT 
TCCTTCAGCT GTGGGAAACA TGAAAGAGGC TATCGGCTAG AAGTTACAAG CACCAACCCG 
CGCCGGGACT ATTTTCATGG CCGCTTCCGG AATGTTCTTC TCACTTCCAT AGCTGTGGTG 
CCGATCCAAA ACCACACTGT GGTCAGCCTT GGCACAGCTG AGGGCCGCGT CATCCAGGTG 
GTGGTTTCCC GCTTTGGCAA GACAGAGCCA CATGTGGACT TCCGCTTGGA CATGCTCCCC 
GTGTCTTCAG AAATGGCCCT GCTGTCTCCA CAGCATCACA ACGGCTCCTT ATTGTTGATA 
ACAGGAGACA AGGTCTCAAA GCTTCCTGTG ATCGGGCCTG GATGTGAGCA GTTGTGGACT 
TGTAGCTCGT GTCTTCTTGC TCCGGGCTTC ATGGGCTGTG GATGGTGCAG GACCAGCAAC 
CGGTGCACCA GGGCCCCTCG ATGCCCCCAG TCCCAATGGA TCCAGGACTC CTGCCCCCTC 
CTCATCACCT CGATCTCTCC TTCCTCCGCT CCACTCAGAG GTCAAACCAA TATCACAATC 
TGTGGCAAAA ACTTTGGCTT TAACAAAAAA GACAGATTTG ATACCAAACT TGTAGATGTG 
GTGGTTGCTG GAACGAAGTG TAAATTGGAA AGGAAGGACA GTAACAATAA TCGGTTGGTC 
TGTGGACTGG ATTATGTGAA CTGGTCCAGC TTGGACTCTG TGATCACAGT GCGCAGTGGC 
AAGGAACAGG CCCAGAAAGA TGGCTTCTCA TTTGTGAATC CAGTTATCAT AGAGATCTTC 
CCAGAGTTTG GACCTCAGTC TGGCGGGACA ATGCTCACTA TCAGCGGTTC ATTCTTGGAC 
AGTGGAAATG TGCAAACAGT CACAGTGGGG AACGCTACCT GTGTGCTGCA GAGTGTTTCA 
GCCACAATGT TAACATGTCG TACACCACCT CAGCCCTCGC CATCCCAACA CAAGGTACAG 
CTGCACATTG ATGGAGTGAT TTTTGAAGCC CCTGTCAGCT ACACCTACAA CAAGAACCCA 
CACATCTCCA GCGTCCAGCC CAAACATTCT TTCATCAGTG GAGGAAGCAC GGTGACAGTG 
AATGGCTTCT ACCTGCACTC AGCTCTTCAG CCTCAGATGG TTCTCACTGC CGCCACTGAG 
GGCAAACTCT TCCAAGTGAC CTGCAGTCAT GATGAGGATA AGAGAAATAT CCTTTGCATC 
ACGCCCTCCC TGAAAGGCCT CAGCGTTCAG CCTCCGGTCG CCACTAAAAT GACCTTCGTC 
CTGGATGGTT TTTCCACTGA TCAGTACGAC CTGCTGTACG TGGAAGATCC CAAATTTGAG 
GAGTTTCAGA AGCCCACTGT CACACCAAGG GGCAAAAAGA ACATTCTGGA GATTAAGGTC 
CCCCCTGTGA ATCAAGAGGC GGTGAAAAAC GGTGAGGTGC TGAGAGTTTC AAATCGGACC 
TGCGAGAGTG TCACTTTGGT GGGCAACACG CTCGAATGCA CCGTACCCAT GGAGCTCCAG 
ACCGCCGCCA AAGAGCTGGA GGTGGAGTGG AAGCAGGCCA CATCATCTGT GATCTTGGGC 
CGTGTGATTT TGGCTCAAGA CCAGGATTAC AGGATACTGA TCACTGGAGG AGTGTGTGTG 
TCCATCCTCC TCCTGCTCCT GATCGCTGTG TTTGTTTGGA TCAAGAGAAA GAAGCACATT 
GATGATTTAG CTAAGACTAT GGTTTGGTAT GACGGCCGGG CTCACATTCC GCACTTGGAC 
ATGTTGGCAA ACGCGAGGAG TGTCAGTCCC ACTGATGAAA TGGTCTCTCA CGAGTCGGTG 
GACTATAGAA CCACTTTGCT TGAGGACCAA AACTTGCCTC TGTCTCAGAC AGAGTCCTGC 
CGGCCTCATC TCTACGCTCA TTCCCATGTG GATCTGTCCC CAATGCTCGG GCCAATGGAA 
GGGGACCTGG CGTCTCCGCT GCTGCCCTCT ACAGCGCCTA TAGATCTAGG CAGCCTCCAT 
CCTGAGCTGC TGAAGRAGGT CCAGCATGTG GTCATCGCAA GAGAAGATCT GCTCTTACAT 
GTCAATGAGA TCATCGGGAG AGGGCACTTC GGCTGCGTGT TTCACGGAAC CCTCCTCGAG 
CCAGATGGCC AGAAGCAGCA CTGCGCCATC AAGTCCTTAA ACCGAATCAC AGATATCGAG 
GAGGTGTCTC AGTTTCTGAA GGAGGGCATC ATCGTGAAGG ATTTCAGCCA TCCCAATGTG 
CTTTCTCTGC TGGGAATCTG CCTGCCCAGC GAGGGTTCGC CTCTCGTCGT GTTGCCTTAC 
ATGAAGCACG GAGATCTGCG CAACTTTATC AGAGATGAAA GTCATAACCC CACAGTGAAG 
GACCTGATGG GTTTCGGGCT GCAGGTGGCT AAAGGAATGG AGTATCTCGC CAGCAAGAAA 
TTTGTTCACC GAGACCTCGC GGCCAGAAAC TGCATGCTGG ATGAGAGCTA CACAGTGAAG 
GTGGCAGATG TTGGCCTGGC CAGAGACGTG TATGATAAAG AATACTACAG CGTACACAAT 
AAGCACGGAG TGAAGCTGCC TGTCAAATGG ATGGCGTTAG AAAGCCTGCA GACACACAAG 
TTTACAACCA AATCGGATGT GTGGTCGTTT GGTGTTTTGC TGTGGGAACT GATGACCCGA 
GGTGCTCCGC CATACTCTGA TGTGAACTCC TTCGACATTA CAGTGTTTCT TCTGCAAGGC 
CGGAGACTGT TACAACCAGA GTTCTGCCCT GATGCACTCT ATAATGTCAT GATTGAGTGC 
TGGCACCCCA AACCCGAGCG TCGACCAACT TTCTTAGAAC TAGTGTCTCG CATCTCCGCT 
ATCTTCTCAA GCTTCAGCGG AGAGCACTAC ATCCTCCTGA ACACCACCTA CGTCAACATC 
GACAAAATGA CACCCTACCC CTCTCTCATA TCATCTCAGA GCAACCTCGA CCGCGACTGC 
TGCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001007125.1
CDS	1..4149
Translation	MTIHYSEAASILIILQSLWWGLNCQCEEPIESSKLDLSVTYDLPYFVSDTPIQKLLEINGTVYVGAVNRLYALSKDLKKKHEYKTGPVHEGPDCKTPTDQCSGCENKPRNINNTNMALLMETFYDLELFSCGSVGNGVCSRHVLEDGPLGAEVTCMYTKKNEGSSHGCPDCLAGPAGTQILNIMSGRVVRFFVANSEPLESNGPRLHHTISIRKMRETQDGFEFFSEQSYMDLAPSLRGNYPLHYVYSFQSGPYVYFLTVQREGGNSKAFHTRIVRMCSSDSEILRYVEMPFECIYSERRRKKRSAQVVFNVLQAAHVAKVGYDFQQEMGLKEGEDVLFAAFARSKPDSPEPTASSAVCLISITDINEFFKVFIQKGYTRKLHHFPGSEEKTFNQTFVGDSFSCGKHERGYRLEVTSTNPRRDYFHGRFRNVLLTSIAVVPIQNHTVVSLGTAEGRVIQVVVSRFGKTEPHVDFRLDMLPVSSEMALLSPQHHNGSLLLITGDKVSKLPVIGPGCEQLWTCSSCLLAPGFMGCGWCRTSNRCTRAPRCPQSQWIQDSCPLLITSISPSSAPLRGQTNITICGKNFGFNKKDRFDTKLVDVVVAGTKCKLERKDSNNNRLVCGLDYVNWSSLDSVITVRSGKEQAQKDGFSFVNPVIIEIFPEFGPQSGGTMLTISGSFLDSGNVQTVTVGNATCVLQSVSATMLTCRTPPQPSPSQHKVQLHIDGVIFEAPVSYTYNKNPHISSVQPKHSFISGGSTVTVNGFYLHSALQPQMVLTAATEGKLFQVTCSHDEDKRNILCITPSLKGLSVQPPVATKMTFVLDGFSTDQYDLLYVEDPKFEEFQKPTVTPRGKKNILEIKVPPVNQEAVKNGEVLRVSNRTCESVTLVGNTLECTVPMELQTAAKELEVEWKQATSSVILGRVILAQDQDYRILITGGVCVSILLLLLIAVFVWIKRKKHIDDLAKTMVWYDGRAHIPHLDMLANARSVSPTDEMVSHESVDYRTTLLEDQNLPLSQTESCRPHLYAHSHVDLSPMLGPMEGDLASPLLPSTAPIDLGSLHPELLKnullVQHVVIAREDLLLHVNEIIGRGHFGCVFHGTLLEPDGQKQHCAIKSLNRITDIEEVSQFLKEGIIVKDFSHPNVLSLLGICLPSEGSPLVVLPYMKHGDLRNFIRDESHNPTVKDLMGFGLQVAKGMEYLASKKFVHRDLAARNCMLDESYTVKVADVGLARDVYDKEYYSVHNKHGVKLPVKWMALESLQTHKFTTKSDVWSFGVLLWELMTRGAPPYSDVNSFDITVFLLQGRRLLQPEFCPDALYNVMIECWHPKPERRPTFLELVSRISAIFSSFSGEHYILLNTTYVNIDKMTPYPSLISSQSNLDRDCCT

Target ORF information:

RefSeq Version	NM_001007124.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio MET proto-oncogene, receptor tyrosine kinase (met), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001007124.1

ORF Insert Sequence:

ATGACAATTC ACTATTCTGA AGCTGCTTCC ATCCTAATCA TCCTTCAGTC GCTGTGGTGG 
GGCTTGAATT GTCAATGTGA GGAACCAATA GAAAGCTCCA AACTCGACCT CTCAGTGACC 
TATGACCTCC CTTACTTTGT GTCTGACACC CCCATTCAGA AGCTGTTGGA AATCAATGGA 
ACAGTGTATG TCGGTGCCGT CAATAGACTT TACGCTCTGT CGAAAGACCT GAAGAAGAAA 
CATGAGTATA AGACTGGACC GGTCCACGAG GGTCCAGACT GCAAGACCCC AACAGATCAA 
TGCAGTGGTT GCGAAAACAA GCCCCGTAAC ATAAACAACA CCAATATGGC CCTGTTAATG 
GAGACGTTCT ATGACCTGGA ACTCTTCAGC TGTGGCTCAG TCGGAAATGG CGTCTGCAGT 
CGTCATGTGT TAGAGGATGG GCCTCTGGGT GCGGAAGTAA CTTGCATGTA CACCAAAAAG 
AATGAAGGCA GCAGCCACGG ATGCCCAGAC TGCCTGGCTG GACCTGCGGG CACTCAGATC 
CTCAACATAA TGAGCGGTCG TGTTGTGAGG TTCTTCGTTG CGAACTCTGA ACCTCTTGAG 
TCAAACGGTC CACGTCTCCA CCACACTATT TCCATTAGGA AGATGCGTGA AACTCAAGAT 
GGCTTTGAGT TCTTTTCCGA GCAGTCCTAC ATGGATTTGG CCCCTTCACT GCGGGGGAAC 
TATCCACTAC ATTATGTCTA CTCTTTCCAG AGTGGTCCTT ATGTATATTT TCTCACCGTC 
CAACGCGAAG GTGGCAACTC GAAAGCTTTC CACACGAGAA TCGTACGCAT GTGTTCTTCA 
GATTCTGAGA TCCTGCGTTA TGTAGAAATG CCCTTTGAGT GCATTTACTC TGAGCGAAGG 
AGAAAAAAGC GTTCGGCTCA AGTGGTTTTC AACGTTCTCC AGGCTGCTCA TGTGGCCAAA 
GTTGGCTATG ACTTTCAGCA GGAGATGGGC TTGAAAGAAG GAGAGGACGT GCTGTTTGCT 
GCCTTTGCCC GGAGCAAACC GGACTCACCA GAGCCCACCG CCAGCTCCGC CGTTTGCCTT 
ATCTCCATCA CGGACATCAA TGAATTCTTC AAGGTTTTCA TTCAGAAGGG TTACACAAGG 
AAACTCCATC ACTTTCCAGG ATCTGAAGAG AAAACCTTCA ACCAGACGTT TGTTGGAGAT 
TCCTTCAGCT GTGGGAAACA TGAAAGAGGC TATCGGCTAG AAGTTACAAG CACCAACCCG 
CGCCGGGACT ATTTTCATGG CCGCTTCCGG AATGTTCTTC TCACTTCCAT AGCTGTGGTG 
CCGATCCAAA ACCACACTGT GGTCAGCCTT GGCACAGCTG AGGGCCGCGT CATCCAGGTG 
GTGGTTTCCC GCTTTGGCAA GACAGAGCCA CATGTGGACT TCCGCTTGGA CATGCTCCCC 
GTGTCTTCAG AAATGGCCCT GCTGTCTCCA CAGCATCACA ACGGCTCCTT ATTGTTGATA 
ACAGGAGACA AGGTCTCAAA GCTTCCTGTG ATCGGGCCTG GATGTGAGCA GTTGTGGACT 
TGTAGCTCGT GTCTTCTTGC TCCGGGCTTC ATGGGCTGTG GATGGTGCAG GACCAGCAAC 
CGGTGCACCA GGGCCCCTCG ATGCCCCCAG TCCCAATGGA TCCAGGACTC CTGCCCCCTC 
CTCATCACCT CGATCTCTCC TTCCTCCGCT CCACTCAGAG GTCAAACCAA TATCACAATC 
TGTGGCAAAA ACTTTGGCTT TAACAAAAAA GACAGATTTG ATACCAAACT TGTAGATGTG 
GTGGTTGCTG GAACGAAGTG TAAATTGGAA AGGAAGGACA GTAACAATAA TCGGTTGGTC 
TGTGGACTGG ATTATGTGAA CTGGTCCAGC TTGGACTCTG TGATCACAGT GCGCAGTGGC 
AAGGAACAGG CCCAGAAAGA TGGCTTCTCA TTTGTGAATC CAGTTATCAT AGAGATCTTC 
CCAGAGTTTG GACCTCAGTC TGGCGGGACA ATGCTCACTA TCAGCGGTTC ATTCTTGGAC 
AGTGGAAATG TGCAAACAGT CACAGTGGGG AACGCTACCT GTGTGCTGCA GAGTGTTTCA 
GCCACAATGT TAACATGTCG TACACCACCT CAGCCCTCGC CATCCCAACA CAAGGTACAG 
CTGCACATTG ATGGAGTGAT TTTTGAAGCC CCTGTCAGCT ACACCTACAA CAAGAACCCA 
CACATCTCCA GCGTCCAGCC CAAACATTCT TTCATCAGTG GAGGAAGCAC GGTGACAGTG 
AATGGCTTCT ACCTGCACTC AGCTCTTCAG CCTCAGATGG TTCTCACTGC CGCCACTGAG 
GGCAAACTCT TCCAAGTGAC CTGCAGTCAT GATGAGGATA AGAGAAATAT CCTTTGCATC 
ACGCCCTCCC TGAAAGGCCT CAGCGTTCAG CCTCCGGTCG CCACTAAAAT GACCTTCGTC 
CTGGATGGTT TTTCCACTGA TCAGTACGAC CTGCTGTACG TGGAAGATCC CAAATTTGAG 
GAGTTTCAGA AGCCCACTGT CACACCAAGG GGCAAAAAGA ACATTCTGGA GATTAAGGTC 
CCCCCTGTGA ATCAAGAGGC GGTGAAAAAC GGTGAGGTGC TGAGAGTTTC AAATCGGACC 
TGCGAGAGTG TCACTTTGGT GGGCAACACG CTCGAATGCA CCGTACCCAT GGAGCTCCAG 
ACCGCCGCCA AAGAGCTGGA GGTGGAGTGG AAGCAGGCCA CATCATCTGT GATCTTGGGC 
CGTGTGATTT TGGCTCAAGA CCAGGATTAC AGGATACTGA TCACTGGAGG AGTGTGTGTG 
TCCATCCTCC TCCTGCTCCT GATCGCTGTG TTTGTTTGGA TCAAGAGAAA GAAGCACATT 
GATGATTTAG CTAAGACTAT GGTTTGGTAT GACGGCCGGG CTCACATTCC GCACTTGGAC 
ATGTTGGCAA ACGCGAGGAG TGTCAGTCCC ACTGATGAAA TGGTCTCTCA CGAGTCGGTG 
GACTATAGAA CCACTTTGCT TGAGGACCAA AACTTGCCTC TGTCTCAGAC AGAGTCCTGC 
CGGCCTCATC TCTACGCTCA TTCCCATGTG GATCTGTCCC CAATGCTCGG GCCAATGGAA 
GGGGACCTGG CGTCTCCGCT GCTGCCCTCT ACAGCGCCTA TAGATCTAGG CAGCCTCCAT 
CCTGAGCTGC TGAAGRAGGT CCAGCATGTG GTCATCGCAA GAGAAGATCT GCTCTTACAT 
GTCAATGAGA TCATCGGGAG AGGGCACTTC GGCTGCGTGT TTCACGGAAC CCTCCTCGAG 
CCAGATGGCC AGAAGCAGCA CTGCGCCATC AAGTCCTTAA ACCGAATCAC AGATATCGAG 
GAGGTGTCTC AGTTTCTGAA GGAGGGCATC ATCGTGAAGG ATTTCAGCCA TCCCAATGTG 
CTTTCTCTGC TGGGAATCTG CCTGCCCAGC GAGGGTTCGC CTCTCGTCGT GTTGCCTTAC 
ATGAAGCACG GAGATCTGCG CAACTTTATC AGAGATGAAA GTCATAACCC CACAGTGAAG 
GACCTGATGG GTTTCGGGCT GCAGGTGGCT AAAGGAATGG AGTATCTCGC CAGCAAGAAA 
TTTGTTCACC GAGACCTCGC GGCCAGAAAC TGCATGCTGG ATGAGAGCTA CACAGTGAAG 
GTGGCAGATG TTGGCCTGGC CAGAGACGTG TATGATAAAG AATACTACAG CGTACACAAT 
AAGCACGGAG TGAAGCTGCC TGTCAAATGG ATGGCGTTAG AAAGCCTGCA GACACACAAG 
TTTACAACCA AATCGGATGT GTGGTCGTTT GGTGTTTTGC TGTGGGAACT GATGACCCGA 
GGTGCTCCGC CATACTCTGA TGTGAACTCC TTCGACATTA CAGTGTTTCT TCTGCAAGGC 
CGGAGACTGT TACAACCAGA GTTCTGCCCT GATGCACTCT ATAATGTCAT GATTGAGTGC 
TGGCACCCCA AACCCGAGCG TCGACCAACT TTCTTAGAAC TAGTGTCTCG CATCTCCGCT 
ATCTTCTCAA GCTTCAGCGG AGAGCACTAC ATCCTCCTGA ACACCACCTA CGTCAACATC 
GACAAAATGA CACCCTACCC CTCTCTCATA TCATCTCAGA GCAACCTCGA CCGCGACTGC 
TGCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

met ( XM_005162994.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005163051.1 Danio rerio MET proto-oncogene, receptor tyrosine kinase (met), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa29330
Clone ID Related Accession (Same CDS sequence)	XM_005162994.3 , XM_005162994.4
Accession Version	XM_005162994.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	hepatocyte growth factor receptor isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005162994.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAATTC ACTATTCTGA AGCTGCTTCC ATCCTAATCA TCCTTCAGTC GCTGTGGTGG 
GGCTTGAATT GTCAATGTGA GGAACCAATA GAAAGCTCCA AACTCGACCT CTCAGTGACC 
TATGACCTCC CTTACTTTGT GTCTGACACC CCCATTCAGA AGCTGTTGGA AATCAATGGA 
ACAGTGTATG TCGGTGCCGT CAATAGACTT TACGCTCTGT CGAAAGACCT GAAGAAGAAA 
CATGAGTATA AGACTGGACC GGTCCATGAG GGTCCAGACT GCAAGACCCC AACAGATCAA 
TGCAGTGGTT GTGAAAACAA GCCCCGTAAC ATAAACAACA CCAATATGGC CCTGTTAATG 
GAGACGTTCT ATGACCTGGA ACTTTTCAGC TGTGGCTCAG TCGGGAATGG CGTCTGCAGT 
CGTCATGTGT TAGAGGATGG GCCTCTGGGT GCGGAAGTAA CTTGCATGTA CACCAAAAAG 
AATGAAGGCA GCAGCCATGG ATGCCCAGAC TGCCTGGCTG GACCTGCGGG CACTCAGATC 
CTCAACATAA TGAGCGGTCG TGTTGTGAGG TTCTTCGTTG CGAACTCTGA ACCTCTTGAG 
TCAAACGGTC CACGTCTCCA CCACACTATT TCCATTAGGA AGATGCGTGA AACTCAAGAT 
GGCTTTGAGT TCTTTTCCGA TCAGTCCTAC ATGGATTTGG CCCCTTCACT GCGGGGGAAC 
TATCCACTAC ATTATGTCTA CTCTTTCCAG AGTGGTCCTT ATGTATATTT TCTCACCGTC 
CAACGCGAAG GTGGCAACTC GAAAGCTTTC CACACGAGAA TCGTACGCAT GTGTTCTTCA 
GATTCTGAGA TCCTGCGTTA TGTAGAAATG CCCTTTGAGT GCATTTACTC TGAGCGAAGG 
AGAAAAAAGC GTTCGGCTCA AGTGGTTTTC AACGTTCTCC AGGCTGCTCA TGTGGCCAAA 
GTTGGCTATG ACTTTCAGCA GGAGATGGGC TTGAAAGAAG GAGAGGACGT GCTGTTTGCT 
GCCTTTGCCC GGAGCAAACC GGACTCACCA GAGCCCACCG CCAGCTCCGC CGTTTGCCTT 
ATCTCCATCA CGGACATCAA TGAATTCTTC AAGGTTTTCA TTCAGAAGGG TTACACAAGG 
AAACTCCATC ACTTTCCAGG ATCTGAAGAG AAAACCTTCA ACCAGACGTT TGTAGGAGAT 
TCCTTCAGCT GTGGGAAACA TGAAAGAGGC TATCGGCTAG AAGTTACAAG CACCAACCCG 
CGCCGGGACT ATTTTCATGG CCGCTTCCGG AATGTTCTTC TCACTTCCAT AGCTGTGGTG 
CCGATCCAAA ACCACACTGT GGTCAGCCTT GGCACAGCTG AAGGCCGCGT CATCCAGGTG 
GTGGTTTCCC GCTTTGGCAA GACAGAGCCA CATGTGGACT TCCGCTTGGA CACGCTCCCT 
GTGTCTTCAG AAATGGCCCT GCTGTCTCCA CAGCATCACA ACGGCTCCTT ATTGTTGATA 
ACAGGAAACA AGGTCTCAAA GCTTCCTGTG ATCGGGCCTG GATGTGAGCA GTTGTGGACT 
TGTAGCTCGT GTCTTCTTGC TCCGGGCTTC ATGGGCTGTG GATGGTGCAG GACCAGCAAC 
CTGTGCACCA GGGCCCCTCG ATGCCCCCAG TCCCAATGGA TCCAGGACTC CTGCCCCCTC 
CTCATCACCT CGATCTCTCC TTCCTCCGCT CCACTCAGAG GTCAAACCAA CATCACAATC 
TGTGGCAAAA ACTTTGGCTT TAACAAAAAA GACAGATTTG ATACCAAACT GATAGACGTG 
GTGGTTGCTG GAACGAAGTG TAAATTGGAA AGGAAGGACA GTAACAATAA TCGGTTGGTC 
TGTGGACTGG ATCATGTGAA CTGGTCCAGC GTGGACTCTG TGGTCACAGT GCGCAGTGGC 
AAGGAACAGG CCCAGAAAGA TGGCTTCTCA TTTGTGAATC CAGTTATCAT AGAGATCTTC 
CCAGAGTTTG GACCTCAGTC TGGTGGGACA ATGCTCACTA TCAGCGGTTC ATTCTTGGAC 
AGTGGAAATG TGCAAACAGT CACAGTGGGG AACGCTACCT GTGTGCTGCA GAGTGTTTCA 
GCCACAATGT TAACATGTCG TACACCACCT CAGCCCTCGC CATCCCAACA CAAGGTACAG 
CTGCACATTG ATGGAGTAAT ATTTGAAGCG CCTGTCAGCT ACACCTACAA CAAGAACCCA 
CACATCTCCA GCGTCCAGCC CAAACATTCT TTCATCAGTG GAGGAAGCAC GGTGACAGTG 
AATGGCTTCT ACCTGCACTC AGCTCTTCAG CCTCAGATGG TTCTCACTGC TGCCACTGAG 
GGCAAACTCT TCCAAGTGAC CTGCAGTCAT GATGAGGATA AGAGAAATAT CCTTTGCATC 
ACGCCCTCCC TGAAAGGCCT CAGCGTTCAG CCTCCGGTCG CCACTAAAAT GACCTTCGTC 
CTGGATGGTT TTTCCACTGA TCAGTACGAC CTGCTGTACG TGGAAGATCC CAAATTTGAG 
GAGTTTCAGA AGCCCACTGT CACACCAAGG GGCAAAAAGA ACATTCTGGA GATTAAGGTC 
CCCCCTGTGA ATCAAGAGGC GGTGAAAAAC GGTGAGGTGC TGAGAGTTTC AAATCGGACC 
TGCGAGAGTG TCACTTTGGT GGGCAACACG CTCGAATGCA CCGTACCCAT GGAGCTCCAG 
ACCGCCGCCA AAGAGCTGGA GGTGGAGTGG AAGCAGGCCA CATCATCTGT GATCTTGGGC 
CGTGTGATTT TGGCTCAAGA CCAGGATTAC AGGATACTGA TCACTGGAGG AGTGTGTGTG 
TCCATCCTCC TCCTGCTCCT GATCGCTGTG TTTGTTTGGA TCAAGAGAAA GAAGCACATT 
AATGATTTAG CTAAGACTAT GGTTTGGTAT GACGGCCGGG CTCACATTCC GCACTTGGAC 
ATGTTGGCAA ACGCGAGGAG TGTCAGTCCC ACTAATGAAA TGGTCTCTCA CGAGTCGGTG 
GACTATAGAA CCACTTTGCT TGAGGACCAA AACTTGCCTC TGTCTCAGAC AGAGTCCTGC 
CGGCCTCATC TCTACGCTCA TTCCCATGTG GATCTGTCCC CAATGCTCGG GCCAATGGAA 
GGGGACCTGG CGTCTCCGCT GCTGCCCTCT ACAGCGCCTA TAGATCTAGG CAGCCTCCAT 
CCTGAGCTGC TGAAGGAGGT CCAGCATGTG GTCATCGCAA GAGAAGATCT GCTCTTACAT 
GTCAATGAGA TCATCGGGAG AGGGCACTTC GGCTGCGTGT TTCATGGAAC CCTCCTCGAG 
CCAGATGGCC AGAAGCAGCA CTGCGCCATC AAGTCCTTAA ACCGAATCAC AGATATCGAG 
GAGGTGTCTC AGTTTCTGAA GGAGGGCATC ATCATGAAGG ATTTCAGCCA TCCCAATGTG 
CTTTCTCTGC TGGGAATCTG CCTGCCCAGC GAGGGTTCGC CTCTCGTCGT GTTGCCTTAC 
ATGAAGCACG GAGATCTGCG CAACTTTATC AGAGATGAAA GTCATAACCC CACAGTGAAG 
GACCTGATGG GTTTCGGGCT GCAGGTGGCT AAAGGAATGG AGTATCTCGC CAGCAAGAAA 
TTTGTTCACC GAGACCTCGC GGCCAGAAAC TGCATGCTGG ATGAGAGCTA CACAGTGAAG 
GTGGCAGATT TTGGCCTGGC CAGAGACGTG TATGATAAAG AATACTACAG CGTACACAAC 
AAGCACGGAG TGAAGCTGCC TGTCAAATGG ATGGCGTTAG AAAGCCTGCA GACACACAAG 
TTCACAACCA AATCGGATGT GTGGTCGTTT GGTGTTTTGC TGTGGGAACT GATGACCCGA 
GGTGCTCCGC CATACTCTGA TGTGAACTCC TTCGACATTA CAGTGTTTCT TCTGCAAGGC 
CGGAGACTGT TACAACCAGA GTTCTGCCCG GATGCACTCT ATAATGTCAT GATTGAGTGC 
TGGCACCCCA AACCCGAGCG TCGACCAACT TTCTCAGAAC TAGTGTCTCG CATCTCCGCC 
ATCTTCTCAA GCTTCAGCGG AGAGCACTAC ATCCTCCTGA ACACCACCTA CGTCAACATC 
GACAAAATGA CACCCTACCC CTCTCTCATA TCATCTCAGA GCAACCTCGA CCGCGACTGC 
TGCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163051.1
CDS	343..4491
Translation	MTIHYSEAASILIILQSLWWGLNCQCEEPIESSKLDLSVTYDLPYFVSDTPIQKLLEINGTVYVGAVNRLYALSKDLKKKHEYKTGPVHEGPDCKTPTDQCSGCENKPRNINNTNMALLMETFYDLELFSCGSVGNGVCSRHVLEDGPLGAEVTCMYTKKNEGSSHGCPDCLAGPAGTQILNIMSGRVVRFFVANSEPLESNGPRLHHTISIRKMRETQDGFEFFSDQSYMDLAPSLRGNYPLHYVYSFQSGPYVYFLTVQREGGNSKAFHTRIVRMCSSDSEILRYVEMPFECIYSERRRKKRSAQVVFNVLQAAHVAKVGYDFQQEMGLKEGEDVLFAAFARSKPDSPEPTASSAVCLISITDINEFFKVFIQKGYTRKLHHFPGSEEKTFNQTFVGDSFSCGKHERGYRLEVTSTNPRRDYFHGRFRNVLLTSIAVVPIQNHTVVSLGTAEGRVIQVVVSRFGKTEPHVDFRLDTLPVSSEMALLSPQHHNGSLLLITGNKVSKLPVIGPGCEQLWTCSSCLLAPGFMGCGWCRTSNLCTRAPRCPQSQWIQDSCPLLITSISPSSAPLRGQTNITICGKNFGFNKKDRFDTKLIDVVVAGTKCKLERKDSNNNRLVCGLDHVNWSSVDSVVTVRSGKEQAQKDGFSFVNPVIIEIFPEFGPQSGGTMLTISGSFLDSGNVQTVTVGNATCVLQSVSATMLTCRTPPQPSPSQHKVQLHIDGVIFEAPVSYTYNKNPHISSVQPKHSFISGGSTVTVNGFYLHSALQPQMVLTAATEGKLFQVTCSHDEDKRNILCITPSLKGLSVQPPVATKMTFVLDGFSTDQYDLLYVEDPKFEEFQKPTVTPRGKKNILEIKVPPVNQEAVKNGEVLRVSNRTCESVTLVGNTLECTVPMELQTAAKELEVEWKQATSSVILGRVILAQDQDYRILITGGVCVSILLLLLIAVFVWIKRKKHINDLAKTMVWYDGRAHIPHLDMLANARSVSPTNEMVSHESVDYRTTLLEDQNLPLSQTESCRPHLYAHSHVDLSPMLGPMEGDLASPLLPSTAPIDLGSLHPELLKEVQHVVIAREDLLLHVNEIIGRGHFGCVFHGTLLEPDGQKQHCAIKSLNRITDIEEVSQFLKEGIIMKDFSHPNVLSLLGICLPSEGSPLVVLPYMKHGDLRNFIRDESHNPTVKDLMGFGLQVAKGMEYLASKKFVHRDLAARNCMLDESYTVKVADFGLARDVYDKEYYSVHNKHGVKLPVKWMALESLQTHKFTTKSDVWSFGVLLWELMTRGAPPYSDVNSFDITVFLLQGRRLLQPEFCPDALYNVMIECWHPKPERRPTFSELVSRISAIFSSFSGEHYILLNTTYVNIDKMTPYPSLISSQSNLDRDCCT

Target ORF information:

RefSeq Version	XM_005162994.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio MET proto-oncogene, receptor tyrosine kinase (met), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162994.3

ORF Insert Sequence:

ATGACAATTC ACTATTCTGA AGCTGCTTCC ATCCTAATCA TCCTTCAGTC GCTGTGGTGG 
GGCTTGAATT GTCAATGTGA GGAACCAATA GAAAGCTCCA AACTCGACCT CTCAGTGACC 
TATGACCTCC CTTACTTTGT GTCTGACACC CCCATTCAGA AGCTGTTGGA AATCAATGGA 
ACAGTGTATG TCGGTGCCGT CAATAGACTT TACGCTCTGT CGAAAGACCT GAAGAAGAAA 
CATGAGTATA AGACTGGACC GGTCCATGAG GGTCCAGACT GCAAGACCCC AACAGATCAA 
TGCAGTGGTT GTGAAAACAA GCCCCGTAAC ATAAACAACA CCAATATGGC CCTGTTAATG 
GAGACGTTCT ATGACCTGGA ACTTTTCAGC TGTGGCTCAG TCGGGAATGG CGTCTGCAGT 
CGTCATGTGT TAGAGGATGG GCCTCTGGGT GCGGAAGTAA CTTGCATGTA CACCAAAAAG 
AATGAAGGCA GCAGCCATGG ATGCCCAGAC TGCCTGGCTG GACCTGCGGG CACTCAGATC 
CTCAACATAA TGAGCGGTCG TGTTGTGAGG TTCTTCGTTG CGAACTCTGA ACCTCTTGAG 
TCAAACGGTC CACGTCTCCA CCACACTATT TCCATTAGGA AGATGCGTGA AACTCAAGAT 
GGCTTTGAGT TCTTTTCCGA TCAGTCCTAC ATGGATTTGG CCCCTTCACT GCGGGGGAAC 
TATCCACTAC ATTATGTCTA CTCTTTCCAG AGTGGTCCTT ATGTATATTT TCTCACCGTC 
CAACGCGAAG GTGGCAACTC GAAAGCTTTC CACACGAGAA TCGTACGCAT GTGTTCTTCA 
GATTCTGAGA TCCTGCGTTA TGTAGAAATG CCCTTTGAGT GCATTTACTC TGAGCGAAGG 
AGAAAAAAGC GTTCGGCTCA AGTGGTTTTC AACGTTCTCC AGGCTGCTCA TGTGGCCAAA 
GTTGGCTATG ACTTTCAGCA GGAGATGGGC TTGAAAGAAG GAGAGGACGT GCTGTTTGCT 
GCCTTTGCCC GGAGCAAACC GGACTCACCA GAGCCCACCG CCAGCTCCGC CGTTTGCCTT 
ATCTCCATCA CGGACATCAA TGAATTCTTC AAGGTTTTCA TTCAGAAGGG TTACACAAGG 
AAACTCCATC ACTTTCCAGG ATCTGAAGAG AAAACCTTCA ACCAGACGTT TGTAGGAGAT 
TCCTTCAGCT GTGGGAAACA TGAAAGAGGC TATCGGCTAG AAGTTACAAG CACCAACCCG 
CGCCGGGACT ATTTTCATGG CCGCTTCCGG AATGTTCTTC TCACTTCCAT AGCTGTGGTG 
CCGATCCAAA ACCACACTGT GGTCAGCCTT GGCACAGCTG AAGGCCGCGT CATCCAGGTG 
GTGGTTTCCC GCTTTGGCAA GACAGAGCCA CATGTGGACT TCCGCTTGGA CACGCTCCCT 
GTGTCTTCAG AAATGGCCCT GCTGTCTCCA CAGCATCACA ACGGCTCCTT ATTGTTGATA 
ACAGGAAACA AGGTCTCAAA GCTTCCTGTG ATCGGGCCTG GATGTGAGCA GTTGTGGACT 
TGTAGCTCGT GTCTTCTTGC TCCGGGCTTC ATGGGCTGTG GATGGTGCAG GACCAGCAAC 
CTGTGCACCA GGGCCCCTCG ATGCCCCCAG TCCCAATGGA TCCAGGACTC CTGCCCCCTC 
CTCATCACCT CGATCTCTCC TTCCTCCGCT CCACTCAGAG GTCAAACCAA CATCACAATC 
TGTGGCAAAA ACTTTGGCTT TAACAAAAAA GACAGATTTG ATACCAAACT GATAGACGTG 
GTGGTTGCTG GAACGAAGTG TAAATTGGAA AGGAAGGACA GTAACAATAA TCGGTTGGTC 
TGTGGACTGG ATCATGTGAA CTGGTCCAGC GTGGACTCTG TGGTCACAGT GCGCAGTGGC 
AAGGAACAGG CCCAGAAAGA TGGCTTCTCA TTTGTGAATC CAGTTATCAT AGAGATCTTC 
CCAGAGTTTG GACCTCAGTC TGGTGGGACA ATGCTCACTA TCAGCGGTTC ATTCTTGGAC 
AGTGGAAATG TGCAAACAGT CACAGTGGGG AACGCTACCT GTGTGCTGCA GAGTGTTTCA 
GCCACAATGT TAACATGTCG TACACCACCT CAGCCCTCGC CATCCCAACA CAAGGTACAG 
CTGCACATTG ATGGAGTAAT ATTTGAAGCG CCTGTCAGCT ACACCTACAA CAAGAACCCA 
CACATCTCCA GCGTCCAGCC CAAACATTCT TTCATCAGTG GAGGAAGCAC GGTGACAGTG 
AATGGCTTCT ACCTGCACTC AGCTCTTCAG CCTCAGATGG TTCTCACTGC TGCCACTGAG 
GGCAAACTCT TCCAAGTGAC CTGCAGTCAT GATGAGGATA AGAGAAATAT CCTTTGCATC 
ACGCCCTCCC TGAAAGGCCT CAGCGTTCAG CCTCCGGTCG CCACTAAAAT GACCTTCGTC 
CTGGATGGTT TTTCCACTGA TCAGTACGAC CTGCTGTACG TGGAAGATCC CAAATTTGAG 
GAGTTTCAGA AGCCCACTGT CACACCAAGG GGCAAAAAGA ACATTCTGGA GATTAAGGTC 
CCCCCTGTGA ATCAAGAGGC GGTGAAAAAC GGTGAGGTGC TGAGAGTTTC AAATCGGACC 
TGCGAGAGTG TCACTTTGGT GGGCAACACG CTCGAATGCA CCGTACCCAT GGAGCTCCAG 
ACCGCCGCCA AAGAGCTGGA GGTGGAGTGG AAGCAGGCCA CATCATCTGT GATCTTGGGC 
CGTGTGATTT TGGCTCAAGA CCAGGATTAC AGGATACTGA TCACTGGAGG AGTGTGTGTG 
TCCATCCTCC TCCTGCTCCT GATCGCTGTG TTTGTTTGGA TCAAGAGAAA GAAGCACATT 
AATGATTTAG CTAAGACTAT GGTTTGGTAT GACGGCCGGG CTCACATTCC GCACTTGGAC 
ATGTTGGCAA ACGCGAGGAG TGTCAGTCCC ACTAATGAAA TGGTCTCTCA CGAGTCGGTG 
GACTATAGAA CCACTTTGCT TGAGGACCAA AACTTGCCTC TGTCTCAGAC AGAGTCCTGC 
CGGCCTCATC TCTACGCTCA TTCCCATGTG GATCTGTCCC CAATGCTCGG GCCAATGGAA 
GGGGACCTGG CGTCTCCGCT GCTGCCCTCT ACAGCGCCTA TAGATCTAGG CAGCCTCCAT 
CCTGAGCTGC TGAAGGAGGT CCAGCATGTG GTCATCGCAA GAGAAGATCT GCTCTTACAT 
GTCAATGAGA TCATCGGGAG AGGGCACTTC GGCTGCGTGT TTCATGGAAC CCTCCTCGAG 
CCAGATGGCC AGAAGCAGCA CTGCGCCATC AAGTCCTTAA ACCGAATCAC AGATATCGAG 
GAGGTGTCTC AGTTTCTGAA GGAGGGCATC ATCATGAAGG ATTTCAGCCA TCCCAATGTG 
CTTTCTCTGC TGGGAATCTG CCTGCCCAGC GAGGGTTCGC CTCTCGTCGT GTTGCCTTAC 
ATGAAGCACG GAGATCTGCG CAACTTTATC AGAGATGAAA GTCATAACCC CACAGTGAAG 
GACCTGATGG GTTTCGGGCT GCAGGTGGCT AAAGGAATGG AGTATCTCGC CAGCAAGAAA 
TTTGTTCACC GAGACCTCGC GGCCAGAAAC TGCATGCTGG ATGAGAGCTA CACAGTGAAG 
GTGGCAGATT TTGGCCTGGC CAGAGACGTG TATGATAAAG AATACTACAG CGTACACAAC 
AAGCACGGAG TGAAGCTGCC TGTCAAATGG ATGGCGTTAG AAAGCCTGCA GACACACAAG 
TTCACAACCA AATCGGATGT GTGGTCGTTT GGTGTTTTGC TGTGGGAACT GATGACCCGA 
GGTGCTCCGC CATACTCTGA TGTGAACTCC TTCGACATTA CAGTGTTTCT TCTGCAAGGC 
CGGAGACTGT TACAACCAGA GTTCTGCCCG GATGCACTCT ATAATGTCAT GATTGAGTGC 
TGGCACCCCA AACCCGAGCG TCGACCAACT TTCTCAGAAC TAGTGTCTCG CATCTCCGCC 
ATCTTCTCAA GCTTCAGCGG AGAGCACTAC ATCCTCCTGA ACACCACCTA CGTCAACATC 
GACAAAATGA CACCCTACCC CTCTCTCATA TCATCTCAGA GCAACCTCGA CCGCGACTGC 
TGCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

met ( XM_005162994.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005163051.1 Danio rerio MET proto-oncogene, receptor tyrosine kinase (met), transcript variant X1, mRNA.

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CloneID	ODa29330
Clone ID Related Accession (Same CDS sequence)	XM_005162994.3 , XM_005162994.4
Accession Version	XM_005162994.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	hepatocyte growth factor receptor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005162994.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAATTC ACTATTCTGA AGCTGCTTCC ATCCTAATCA TCCTTCAGTC GCTGTGGTGG 
GGCTTGAATT GTCAATGTGA GGAACCAATA GAAAGCTCCA AACTCGACCT CTCAGTGACC 
TATGACCTCC CTTACTTTGT GTCTGACACC CCCATTCAGA AGCTGTTGGA AATCAATGGA 
ACAGTGTATG TCGGTGCCGT CAATAGACTT TACGCTCTGT CGAAAGACCT GAAGAAGAAA 
CATGAGTATA AGACTGGACC GGTCCATGAG GGTCCAGACT GCAAGACCCC AACAGATCAA 
TGCAGTGGTT GTGAAAACAA GCCCCGTAAC ATAAACAACA CCAATATGGC CCTGTTAATG 
GAGACGTTCT ATGACCTGGA ACTTTTCAGC TGTGGCTCAG TCGGGAATGG CGTCTGCAGT 
CGTCATGTGT TAGAGGATGG GCCTCTGGGT GCGGAAGTAA CTTGCATGTA CACCAAAAAG 
AATGAAGGCA GCAGCCATGG ATGCCCAGAC TGCCTGGCTG GACCTGCGGG CACTCAGATC 
CTCAACATAA TGAGCGGTCG TGTTGTGAGG TTCTTCGTTG CGAACTCTGA ACCTCTTGAG 
TCAAACGGTC CACGTCTCCA CCACACTATT TCCATTAGGA AGATGCGTGA AACTCAAGAT 
GGCTTTGAGT TCTTTTCCGA TCAGTCCTAC ATGGATTTGG CCCCTTCACT GCGGGGGAAC 
TATCCACTAC ATTATGTCTA CTCTTTCCAG AGTGGTCCTT ATGTATATTT TCTCACCGTC 
CAACGCGAAG GTGGCAACTC GAAAGCTTTC CACACGAGAA TCGTACGCAT GTGTTCTTCA 
GATTCTGAGA TCCTGCGTTA TGTAGAAATG CCCTTTGAGT GCATTTACTC TGAGCGAAGG 
AGAAAAAAGC GTTCGGCTCA AGTGGTTTTC AACGTTCTCC AGGCTGCTCA TGTGGCCAAA 
GTTGGCTATG ACTTTCAGCA GGAGATGGGC TTGAAAGAAG GAGAGGACGT GCTGTTTGCT 
GCCTTTGCCC GGAGCAAACC GGACTCACCA GAGCCCACCG CCAGCTCCGC CGTTTGCCTT 
ATCTCCATCA CGGACATCAA TGAATTCTTC AAGGTTTTCA TTCAGAAGGG TTACACAAGG 
AAACTCCATC ACTTTCCAGG ATCTGAAGAG AAAACCTTCA ACCAGACGTT TGTAGGAGAT 
TCCTTCAGCT GTGGGAAACA TGAAAGAGGC TATCGGCTAG AAGTTACAAG CACCAACCCG 
CGCCGGGACT ATTTTCATGG CCGCTTCCGG AATGTTCTTC TCACTTCCAT AGCTGTGGTG 
CCGATCCAAA ACCACACTGT GGTCAGCCTT GGCACAGCTG AAGGCCGCGT CATCCAGGTG 
GTGGTTTCCC GCTTTGGCAA GACAGAGCCA CATGTGGACT TCCGCTTGGA CACGCTCCCT 
GTGTCTTCAG AAATGGCCCT GCTGTCTCCA CAGCATCACA ACGGCTCCTT ATTGTTGATA 
ACAGGAAACA AGGTCTCAAA GCTTCCTGTG ATCGGGCCTG GATGTGAGCA GTTGTGGACT 
TGTAGCTCGT GTCTTCTTGC TCCGGGCTTC ATGGGCTGTG GATGGTGCAG GACCAGCAAC 
CTGTGCACCA GGGCCCCTCG ATGCCCCCAG TCCCAATGGA TCCAGGACTC CTGCCCCCTC 
CTCATCACCT CGATCTCTCC TTCCTCCGCT CCACTCAGAG GTCAAACCAA CATCACAATC 
TGTGGCAAAA ACTTTGGCTT TAACAAAAAA GACAGATTTG ATACCAAACT GATAGACGTG 
GTGGTTGCTG GAACGAAGTG TAAATTGGAA AGGAAGGACA GTAACAATAA TCGGTTGGTC 
TGTGGACTGG ATCATGTGAA CTGGTCCAGC GTGGACTCTG TGGTCACAGT GCGCAGTGGC 
AAGGAACAGG CCCAGAAAGA TGGCTTCTCA TTTGTGAATC CAGTTATCAT AGAGATCTTC 
CCAGAGTTTG GACCTCAGTC TGGTGGGACA ATGCTCACTA TCAGCGGTTC ATTCTTGGAC 
AGTGGAAATG TGCAAACAGT CACAGTGGGG AACGCTACCT GTGTGCTGCA GAGTGTTTCA 
GCCACAATGT TAACATGTCG TACACCACCT CAGCCCTCGC CATCCCAACA CAAGGTACAG 
CTGCACATTG ATGGAGTAAT ATTTGAAGCG CCTGTCAGCT ACACCTACAA CAAGAACCCA 
CACATCTCCA GCGTCCAGCC CAAACATTCT TTCATCAGTG GAGGAAGCAC GGTGACAGTG 
AATGGCTTCT ACCTGCACTC AGCTCTTCAG CCTCAGATGG TTCTCACTGC TGCCACTGAG 
GGCAAACTCT TCCAAGTGAC CTGCAGTCAT GATGAGGATA AGAGAAATAT CCTTTGCATC 
ACGCCCTCCC TGAAAGGCCT CAGCGTTCAG CCTCCGGTCG CCACTAAAAT GACCTTCGTC 
CTGGATGGTT TTTCCACTGA TCAGTACGAC CTGCTGTACG TGGAAGATCC CAAATTTGAG 
GAGTTTCAGA AGCCCACTGT CACACCAAGG GGCAAAAAGA ACATTCTGGA GATTAAGGTC 
CCCCCTGTGA ATCAAGAGGC GGTGAAAAAC GGTGAGGTGC TGAGAGTTTC AAATCGGACC 
TGCGAGAGTG TCACTTTGGT GGGCAACACG CTCGAATGCA CCGTACCCAT GGAGCTCCAG 
ACCGCCGCCA AAGAGCTGGA GGTGGAGTGG AAGCAGGCCA CATCATCTGT GATCTTGGGC 
CGTGTGATTT TGGCTCAAGA CCAGGATTAC AGGATACTGA TCACTGGAGG AGTGTGTGTG 
TCCATCCTCC TCCTGCTCCT GATCGCTGTG TTTGTTTGGA TCAAGAGAAA GAAGCACATT 
AATGATTTAG CTAAGACTAT GGTTTGGTAT GACGGCCGGG CTCACATTCC GCACTTGGAC 
ATGTTGGCAA ACGCGAGGAG TGTCAGTCCC ACTAATGAAA TGGTCTCTCA CGAGTCGGTG 
GACTATAGAA CCACTTTGCT TGAGGACCAA AACTTGCCTC TGTCTCAGAC AGAGTCCTGC 
CGGCCTCATC TCTACGCTCA TTCCCATGTG GATCTGTCCC CAATGCTCGG GCCAATGGAA 
GGGGACCTGG CGTCTCCGCT GCTGCCCTCT ACAGCGCCTA TAGATCTAGG CAGCCTCCAT 
CCTGAGCTGC TGAAGGAGGT CCAGCATGTG GTCATCGCAA GAGAAGATCT GCTCTTACAT 
GTCAATGAGA TCATCGGGAG AGGGCACTTC GGCTGCGTGT TTCATGGAAC CCTCCTCGAG 
CCAGATGGCC AGAAGCAGCA CTGCGCCATC AAGTCCTTAA ACCGAATCAC AGATATCGAG 
GAGGTGTCTC AGTTTCTGAA GGAGGGCATC ATCATGAAGG ATTTCAGCCA TCCCAATGTG 
CTTTCTCTGC TGGGAATCTG CCTGCCCAGC GAGGGTTCGC CTCTCGTCGT GTTGCCTTAC 
ATGAAGCACG GAGATCTGCG CAACTTTATC AGAGATGAAA GTCATAACCC CACAGTGAAG 
GACCTGATGG GTTTCGGGCT GCAGGTGGCT AAAGGAATGG AGTATCTCGC CAGCAAGAAA 
TTTGTTCACC GAGACCTCGC GGCCAGAAAC TGCATGCTGG ATGAGAGCTA CACAGTGAAG 
GTGGCAGATT TTGGCCTGGC CAGAGACGTG TATGATAAAG AATACTACAG CGTACACAAC 
AAGCACGGAG TGAAGCTGCC TGTCAAATGG ATGGCGTTAG AAAGCCTGCA GACACACAAG 
TTCACAACCA AATCGGATGT GTGGTCGTTT GGTGTTTTGC TGTGGGAACT GATGACCCGA 
GGTGCTCCGC CATACTCTGA TGTGAACTCC TTCGACATTA CAGTGTTTCT TCTGCAAGGC 
CGGAGACTGT TACAACCAGA GTTCTGCCCG GATGCACTCT ATAATGTCAT GATTGAGTGC 
TGGCACCCCA AACCCGAGCG TCGACCAACT TTCTCAGAAC TAGTGTCTCG CATCTCCGCC 
ATCTTCTCAA GCTTCAGCGG AGAGCACTAC ATCCTCCTGA ACACCACCTA CGTCAACATC 
GACAAAATGA CACCCTACCC CTCTCTCATA TCATCTCAGA GCAACCTCGA CCGCGACTGC 
TGCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163051.1
CDS	368..4516
Translation	MTIHYSEAASILIILQSLWWGLNCQCEEPIESSKLDLSVTYDLPYFVSDTPIQKLLEINGTVYVGAVNRLYALSKDLKKKHEYKTGPVHEGPDCKTPTDQCSGCENKPRNINNTNMALLMETFYDLELFSCGSVGNGVCSRHVLEDGPLGAEVTCMYTKKNEGSSHGCPDCLAGPAGTQILNIMSGRVVRFFVANSEPLESNGPRLHHTISIRKMRETQDGFEFFSDQSYMDLAPSLRGNYPLHYVYSFQSGPYVYFLTVQREGGNSKAFHTRIVRMCSSDSEILRYVEMPFECIYSERRRKKRSAQVVFNVLQAAHVAKVGYDFQQEMGLKEGEDVLFAAFARSKPDSPEPTASSAVCLISITDINEFFKVFIQKGYTRKLHHFPGSEEKTFNQTFVGDSFSCGKHERGYRLEVTSTNPRRDYFHGRFRNVLLTSIAVVPIQNHTVVSLGTAEGRVIQVVVSRFGKTEPHVDFRLDTLPVSSEMALLSPQHHNGSLLLITGNKVSKLPVIGPGCEQLWTCSSCLLAPGFMGCGWCRTSNLCTRAPRCPQSQWIQDSCPLLITSISPSSAPLRGQTNITICGKNFGFNKKDRFDTKLIDVVVAGTKCKLERKDSNNNRLVCGLDHVNWSSVDSVVTVRSGKEQAQKDGFSFVNPVIIEIFPEFGPQSGGTMLTISGSFLDSGNVQTVTVGNATCVLQSVSATMLTCRTPPQPSPSQHKVQLHIDGVIFEAPVSYTYNKNPHISSVQPKHSFISGGSTVTVNGFYLHSALQPQMVLTAATEGKLFQVTCSHDEDKRNILCITPSLKGLSVQPPVATKMTFVLDGFSTDQYDLLYVEDPKFEEFQKPTVTPRGKKNILEIKVPPVNQEAVKNGEVLRVSNRTCESVTLVGNTLECTVPMELQTAAKELEVEWKQATSSVILGRVILAQDQDYRILITGGVCVSILLLLLIAVFVWIKRKKHINDLAKTMVWYDGRAHIPHLDMLANARSVSPTNEMVSHESVDYRTTLLEDQNLPLSQTESCRPHLYAHSHVDLSPMLGPMEGDLASPLLPSTAPIDLGSLHPELLKEVQHVVIAREDLLLHVNEIIGRGHFGCVFHGTLLEPDGQKQHCAIKSLNRITDIEEVSQFLKEGIIMKDFSHPNVLSLLGICLPSEGSPLVVLPYMKHGDLRNFIRDESHNPTVKDLMGFGLQVAKGMEYLASKKFVHRDLAARNCMLDESYTVKVADFGLARDVYDKEYYSVHNKHGVKLPVKWMALESLQTHKFTTKSDVWSFGVLLWELMTRGAPPYSDVNSFDITVFLLQGRRLLQPEFCPDALYNVMIECWHPKPERRPTFSELVSRISAIFSSFSGEHYILLNTTYVNIDKMTPYPSLISSQSNLDRDCCT

Target ORF information:

RefSeq Version	XM_005162994.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio MET proto-oncogene, receptor tyrosine kinase (met), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162994.4

ORF Insert Sequence:

ATGACAATTC ACTATTCTGA AGCTGCTTCC ATCCTAATCA TCCTTCAGTC GCTGTGGTGG 
GGCTTGAATT GTCAATGTGA GGAACCAATA GAAAGCTCCA AACTCGACCT CTCAGTGACC 
TATGACCTCC CTTACTTTGT GTCTGACACC CCCATTCAGA AGCTGTTGGA AATCAATGGA 
ACAGTGTATG TCGGTGCCGT CAATAGACTT TACGCTCTGT CGAAAGACCT GAAGAAGAAA 
CATGAGTATA AGACTGGACC GGTCCATGAG GGTCCAGACT GCAAGACCCC AACAGATCAA 
TGCAGTGGTT GTGAAAACAA GCCCCGTAAC ATAAACAACA CCAATATGGC CCTGTTAATG 
GAGACGTTCT ATGACCTGGA ACTTTTCAGC TGTGGCTCAG TCGGGAATGG CGTCTGCAGT 
CGTCATGTGT TAGAGGATGG GCCTCTGGGT GCGGAAGTAA CTTGCATGTA CACCAAAAAG 
AATGAAGGCA GCAGCCATGG ATGCCCAGAC TGCCTGGCTG GACCTGCGGG CACTCAGATC 
CTCAACATAA TGAGCGGTCG TGTTGTGAGG TTCTTCGTTG CGAACTCTGA ACCTCTTGAG 
TCAAACGGTC CACGTCTCCA CCACACTATT TCCATTAGGA AGATGCGTGA AACTCAAGAT 
GGCTTTGAGT TCTTTTCCGA TCAGTCCTAC ATGGATTTGG CCCCTTCACT GCGGGGGAAC 
TATCCACTAC ATTATGTCTA CTCTTTCCAG AGTGGTCCTT ATGTATATTT TCTCACCGTC 
CAACGCGAAG GTGGCAACTC GAAAGCTTTC CACACGAGAA TCGTACGCAT GTGTTCTTCA 
GATTCTGAGA TCCTGCGTTA TGTAGAAATG CCCTTTGAGT GCATTTACTC TGAGCGAAGG 
AGAAAAAAGC GTTCGGCTCA AGTGGTTTTC AACGTTCTCC AGGCTGCTCA TGTGGCCAAA 
GTTGGCTATG ACTTTCAGCA GGAGATGGGC TTGAAAGAAG GAGAGGACGT GCTGTTTGCT 
GCCTTTGCCC GGAGCAAACC GGACTCACCA GAGCCCACCG CCAGCTCCGC CGTTTGCCTT 
ATCTCCATCA CGGACATCAA TGAATTCTTC AAGGTTTTCA TTCAGAAGGG TTACACAAGG 
AAACTCCATC ACTTTCCAGG ATCTGAAGAG AAAACCTTCA ACCAGACGTT TGTAGGAGAT 
TCCTTCAGCT GTGGGAAACA TGAAAGAGGC TATCGGCTAG AAGTTACAAG CACCAACCCG 
CGCCGGGACT ATTTTCATGG CCGCTTCCGG AATGTTCTTC TCACTTCCAT AGCTGTGGTG 
CCGATCCAAA ACCACACTGT GGTCAGCCTT GGCACAGCTG AAGGCCGCGT CATCCAGGTG 
GTGGTTTCCC GCTTTGGCAA GACAGAGCCA CATGTGGACT TCCGCTTGGA CACGCTCCCT 
GTGTCTTCAG AAATGGCCCT GCTGTCTCCA CAGCATCACA ACGGCTCCTT ATTGTTGATA 
ACAGGAAACA AGGTCTCAAA GCTTCCTGTG ATCGGGCCTG GATGTGAGCA GTTGTGGACT 
TGTAGCTCGT GTCTTCTTGC TCCGGGCTTC ATGGGCTGTG GATGGTGCAG GACCAGCAAC 
CTGTGCACCA GGGCCCCTCG ATGCCCCCAG TCCCAATGGA TCCAGGACTC CTGCCCCCTC 
CTCATCACCT CGATCTCTCC TTCCTCCGCT CCACTCAGAG GTCAAACCAA CATCACAATC 
TGTGGCAAAA ACTTTGGCTT TAACAAAAAA GACAGATTTG ATACCAAACT GATAGACGTG 
GTGGTTGCTG GAACGAAGTG TAAATTGGAA AGGAAGGACA GTAACAATAA TCGGTTGGTC 
TGTGGACTGG ATCATGTGAA CTGGTCCAGC GTGGACTCTG TGGTCACAGT GCGCAGTGGC 
AAGGAACAGG CCCAGAAAGA TGGCTTCTCA TTTGTGAATC CAGTTATCAT AGAGATCTTC 
CCAGAGTTTG GACCTCAGTC TGGTGGGACA ATGCTCACTA TCAGCGGTTC ATTCTTGGAC 
AGTGGAAATG TGCAAACAGT CACAGTGGGG AACGCTACCT GTGTGCTGCA GAGTGTTTCA 
GCCACAATGT TAACATGTCG TACACCACCT CAGCCCTCGC CATCCCAACA CAAGGTACAG 
CTGCACATTG ATGGAGTAAT ATTTGAAGCG CCTGTCAGCT ACACCTACAA CAAGAACCCA 
CACATCTCCA GCGTCCAGCC CAAACATTCT TTCATCAGTG GAGGAAGCAC GGTGACAGTG 
AATGGCTTCT ACCTGCACTC AGCTCTTCAG CCTCAGATGG TTCTCACTGC TGCCACTGAG 
GGCAAACTCT TCCAAGTGAC CTGCAGTCAT GATGAGGATA AGAGAAATAT CCTTTGCATC 
ACGCCCTCCC TGAAAGGCCT CAGCGTTCAG CCTCCGGTCG CCACTAAAAT GACCTTCGTC 
CTGGATGGTT TTTCCACTGA TCAGTACGAC CTGCTGTACG TGGAAGATCC CAAATTTGAG 
GAGTTTCAGA AGCCCACTGT CACACCAAGG GGCAAAAAGA ACATTCTGGA GATTAAGGTC 
CCCCCTGTGA ATCAAGAGGC GGTGAAAAAC GGTGAGGTGC TGAGAGTTTC AAATCGGACC 
TGCGAGAGTG TCACTTTGGT GGGCAACACG CTCGAATGCA CCGTACCCAT GGAGCTCCAG 
ACCGCCGCCA AAGAGCTGGA GGTGGAGTGG AAGCAGGCCA CATCATCTGT GATCTTGGGC 
CGTGTGATTT TGGCTCAAGA CCAGGATTAC AGGATACTGA TCACTGGAGG AGTGTGTGTG 
TCCATCCTCC TCCTGCTCCT GATCGCTGTG TTTGTTTGGA TCAAGAGAAA GAAGCACATT 
AATGATTTAG CTAAGACTAT GGTTTGGTAT GACGGCCGGG CTCACATTCC GCACTTGGAC 
ATGTTGGCAA ACGCGAGGAG TGTCAGTCCC ACTAATGAAA TGGTCTCTCA CGAGTCGGTG 
GACTATAGAA CCACTTTGCT TGAGGACCAA AACTTGCCTC TGTCTCAGAC AGAGTCCTGC 
CGGCCTCATC TCTACGCTCA TTCCCATGTG GATCTGTCCC CAATGCTCGG GCCAATGGAA 
GGGGACCTGG CGTCTCCGCT GCTGCCCTCT ACAGCGCCTA TAGATCTAGG CAGCCTCCAT 
CCTGAGCTGC TGAAGGAGGT CCAGCATGTG GTCATCGCAA GAGAAGATCT GCTCTTACAT 
GTCAATGAGA TCATCGGGAG AGGGCACTTC GGCTGCGTGT TTCATGGAAC CCTCCTCGAG 
CCAGATGGCC AGAAGCAGCA CTGCGCCATC AAGTCCTTAA ACCGAATCAC AGATATCGAG 
GAGGTGTCTC AGTTTCTGAA GGAGGGCATC ATCATGAAGG ATTTCAGCCA TCCCAATGTG 
CTTTCTCTGC TGGGAATCTG CCTGCCCAGC GAGGGTTCGC CTCTCGTCGT GTTGCCTTAC 
ATGAAGCACG GAGATCTGCG CAACTTTATC AGAGATGAAA GTCATAACCC CACAGTGAAG 
GACCTGATGG GTTTCGGGCT GCAGGTGGCT AAAGGAATGG AGTATCTCGC CAGCAAGAAA 
TTTGTTCACC GAGACCTCGC GGCCAGAAAC TGCATGCTGG ATGAGAGCTA CACAGTGAAG 
GTGGCAGATT TTGGCCTGGC CAGAGACGTG TATGATAAAG AATACTACAG CGTACACAAC 
AAGCACGGAG TGAAGCTGCC TGTCAAATGG ATGGCGTTAG AAAGCCTGCA GACACACAAG 
TTCACAACCA AATCGGATGT GTGGTCGTTT GGTGTTTTGC TGTGGGAACT GATGACCCGA 
GGTGCTCCGC CATACTCTGA TGTGAACTCC TTCGACATTA CAGTGTTTCT TCTGCAAGGC 
CGGAGACTGT TACAACCAGA GTTCTGCCCG GATGCACTCT ATAATGTCAT GATTGAGTGC 
TGGCACCCCA AACCCGAGCG TCGACCAACT TTCTCAGAAC TAGTGTCTCG CATCTCCGCC 
ATCTTCTCAA GCTTCAGCGG AGAGCACTAC ATCCTCCTGA ACACCACCTA CGTCAACATC 
GACAAAATGA CACCCTACCC CTCTCTCATA TCATCTCAGA GCAACCTCGA CCGCGACTGC 
TGCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

met ( XM_005162995.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005163052.1 Danio rerio MET proto-oncogene, receptor tyrosine kinase (met), transcript variant X2, mRNA.

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CloneID	ODa29331
Clone ID Related Accession (Same CDS sequence)	XM_005162995.4 , XM_005162995.3
Accession Version	XM_005162995.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	hepatocyte growth factor receptor isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005162995.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAATTC ACTATTCTGA AGCTGCTTCC ATCCTAATCA TCCTTCAGTC GCTGTGGTGG 
GGCTTGAATT GTCAATGTGA GGAACCAATA GAAAGCTCCA AACTCGACCT CTCAGTGACC 
TATGACCTCC CTTACTTTGT GTCTGACACC CCCATTCAGA AGCTGTTGGA AATCAATGGA 
ACAGTGTATG TCGGTGCCGT CAATAGACTT TACGCTCTGT CGAAAGACCT GAAGAAGAAA 
CATGAGTATA AGACTGGACC GGTCCATGAG GGTCCAGACT GCAAGACCCC AACAGATCAA 
TGCAGTGGTT GTGAAAACAA GCCCCGTAAC ATAAACAACA CCAATATGGC CCTGTTAATG 
GAGACGTTCT ATGACCTGGA ACTTTTCAGC TGTGGCTCAG TCGGGAATGG CGTCTGCAGT 
CGTCATGTGT TAGAGGATGG GCCTCTGGGT GCGGAAGTAA CTTGCATGTA CACCAAAAAG 
AATGAAGGCA GCAGCCATGG ATGCCCAGAC TGCCTGGCTG GACCTGCGGG CACTCAGATC 
CTCAACATAA TGAGCGGTCG TGTTGTGAGG TTCTTCGTTG CGAACTCTGA ACCTCTTGAG 
TCAAACGGTC CACGTCTCCA CCACACTATT TCCATTAGGA AGATGCGTGA AACTCAAGAT 
GGCTTTGAGT TCTTTTCCGA TCAGTCCTAC ATGGATTTGG CCCCTTCACT GCGGGGGAAC 
TATCCACTAC ATTATGTCTA CTCTTTCCAG AGTGGTCCTT ATGTATATTT TCTCACCGTC 
CAACGCGAAG GTGGCAACTC GAAAGCTTTC CACACGAGAA TCGTACGCAT GTGTTCTTCA 
GATTCTGAGA TCCTGCGTTA TGTAGAAATG CCCTTTGAGT GCATTTACTC TGAGCGAAGG 
AGAAAAAAGC GTTCGGCTCA AGTGGTTTTC AACGTTCTCC AGGCTGCTCA TGTGGCCAAA 
GTTGGCTATG ACTTTCAGCA GGAGATGGGC TTGAAAGAAG GAGAGGACGT GCTGTTTGCT 
GCCTTTGCCC GGAGCAAACC GGACTCACCA GAGCCCACCG CCAGCTCCGC CGTTTGCCTT 
ATCTCCATCA CGGACATCAA TGAATTCTTC AAGGTTTTCA TTCAGAAGGG TTACACAAGG 
AAACTCCATC ACTTTCCAGG ATCTGAAGAG AAAACCTTCA ACCAGACGTT TGTAGGAGAT 
TCCTTCAGCT GTGGGAAACA TGAAAGAGGC TATCGGCTAG AAGTTACAAG CACCAACCCG 
CGCCGGGACT ATTTTCATGG CCGCTTCCGG AATGTTCTTC TCACTTCCAT AGCTGTGGTG 
CCGATCCAAA ACCACACTGT GGTCAGCCTT GGCACAGCTG AAGGCCGCGT CATCCAGGTG 
GTGGTTTCCC GCTTTGGCAA GACAGAGCCA CATGTGGACT TCCGCTTGGA CACGCTCCCT 
GTGTCTTCAG AAATGGCCCT GCTGTCTCCA CAGCATCACA ACGGCTCCTT ATTGTTGATA 
ACAGGAAACA AGGTCTCAAA GCTTCCTGTG ATCGGGCCTG GATGTGAGCA GTTGTGGACT 
TGTAGCTCGT GTCTTCTTGC TCCGGGCTTC ATGGGCTGTG GATGGTGCAG GACCAGCAAC 
CTGTGCACCA GGGCCCCTCG ATGCCCCCAG TCCCAATGGA TCCAGGACTC CTGCCCCCTC 
CTCATCACCT CGATCTCTCC TTCCTCCGCT CCACTCAGAG GTCAAACCAA CATCACAATC 
TGTGGCAAAA ACTTTGGCTT TAACAAAAAA GACAGATTTG ATACCAAACT GATAGACGTG 
GTGGTTGCTG GAACGAAGTG TAAATTGGAA AGGAAGGACA GTAACAATAA TCGGTTGGTC 
TGTGGACTGG ATCATGTGAA CTGGTCCAGC GTGGACTCTG TGGTCACAGT GCGCAGTGGC 
AAGGAACAGG CCCAGAAAGA TGGCTTCTCA TTTGTGAATC CAGTTATCAT AGAGATCTTC 
CCAGAGTTTG GACCTCAGTC TGGTGGGACA ATGCTCACTA TCAGCGGTTC ATTCTTGGAC 
AGTGGAAATG TGCAAACAGT CACAGTGGGG AACGCTACCT GTGTGCTGCA GAGTGTTTCA 
GCCACAATGT TAACATGTCG TACACCACCT CAGCCCTCGC CATCCCAACA CAAGGTACAG 
CTGCACATTG ATGGAGTAAT ATTTGAAGCG CCTGTCAGCT ACACCTACAA CAAGAACCCA 
CACATCTCCA GCGTCCAGCC CAAACATTCT TTCATCAGTG GAGGAAGCAC GGTGACAGTG 
AATGGCTTCT ACCTGCACTC AGCTCTTCAG CCTCAGATGG TTCTCACTGC TGCCACTGAG 
GGCAAACTCT TCCAAGTGAC CTGCAGTCAT GATGAGGATA AGAGAAATAT CCTTTGCATC 
ACGCCCTCCC TGAAAGGCCT CAGCGTTCAG CCTCCGGTCG CCACTAAAAT GACCTTCGTC 
CTGGATGGTT TTTCCACTGA TCAGTACGAC CTGCTGTACG TGGAAGATCC CAAATTTGAG 
GAGTTTCAGA AGCCCACTGT CACACCAAGG GGCAAAAAGA ACATTCTGGA GATTAAGGTC 
CCCCCTGTGA ATCAAGAGGC GGTGAAAAAC GGTGAGGTGC TGAGAGTTTC AAATCGGACC 
TGCGAGAGTG TCACTTTGGT GGGCAACACG CTCGAATGCA CCGTACCCAT GGAGCTCCAG 
ACCGCCGCCA AAGAGCTGGA GGTGGAGTGG AAGCAGGCCA CATCATCTGT GATCTTGGGC 
CGTGTGATTT TGGCTCAAGA CCAGGATTAC AGGATACTGA TCACTGGAGG AGTGTGTGTG 
TCCATCCTCC TCCTGCTCCT GATCGCTGTG TTTGTTTGGA TCAAGAGAAA GAAGCACATT 
AATGACCAAA ACTTGCCTCT GTCTCAGACA GAGTCCTGCC GGCCTCATCT CTACGCTCAT 
TCCCATGTGG ATCTGTCCCC AATGCTCGGG CCAATGGAAG GGGACCTGGC GTCTCCGCTG 
CTGCCCTCTA CAGCGCCTAT AGATCTAGGC AGCCTCCATC CTGAGCTGCT GAAGGAGGTC 
CAGCATGTGG TCATCGCAAG AGAAGATCTG CTCTTACATG TCAATGAGAT CATCGGGAGA 
GGGCACTTCG GCTGCGTGTT TCATGGAACC CTCCTCGAGC CAGATGGCCA GAAGCAGCAC 
TGCGCCATCA AGTCCTTAAA CCGAATCACA GATATCGAGG AGGTGTCTCA GTTTCTGAAG 
GAGGGCATCA TCATGAAGGA TTTCAGCCAT CCCAATGTGC TTTCTCTGCT GGGAATCTGC 
CTGCCCAGCG AGGGTTCGCC TCTCGTCGTG TTGCCTTACA TGAAGCACGG AGATCTGCGC 
AACTTTATCA GAGATGAAAG TCATAACCCC ACAGTGAAGG ACCTGATGGG TTTCGGGCTG 
CAGGTGGCTA AAGGAATGGA GTATCTCGCC AGCAAGAAAT TTGTTCACCG AGACCTCGCG 
GCCAGAAACT GCATGCTGGA TGAGAGCTAC ACAGTGAAGG TGGCAGATTT TGGCCTGGCC 
AGAGACGTGT ATGATAAAGA ATACTACAGC GTACACAACA AGCACGGAGT GAAGCTGCCT 
GTCAAATGGA TGGCGTTAGA AAGCCTGCAG ACACACAAGT TCACAACCAA ATCGGATGTG 
TGGTCGTTTG GTGTTTTGCT GTGGGAACTG ATGACCCGAG GTGCTCCGCC ATACTCTGAT 
GTGAACTCCT TCGACATTAC AGTGTTTCTT CTGCAAGGCC GGAGACTGTT ACAACCAGAG 
TTCTGCCCGG ATGCACTCTA TAATGTCATG ATTGAGTGCT GGCACCCCAA ACCCGAGCGT 
CGACCAACTT TCTCAGAACT AGTGTCTCGC ATCTCCGCCA TCTTCTCAAG CTTCAGCGGA 
GAGCACTACA TCCTCCTGAA CACCACCTAC GTCAACATCG ACAAAATGAC ACCCTACCCC 
TCTCTCATAT CATCTCAGAG CAACCTCGAC CGCGACTGCT GCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163052.1
CDS	369..4376
Translation	MTIHYSEAASILIILQSLWWGLNCQCEEPIESSKLDLSVTYDLPYFVSDTPIQKLLEINGTVYVGAVNRLYALSKDLKKKHEYKTGPVHEGPDCKTPTDQCSGCENKPRNINNTNMALLMETFYDLELFSCGSVGNGVCSRHVLEDGPLGAEVTCMYTKKNEGSSHGCPDCLAGPAGTQILNIMSGRVVRFFVANSEPLESNGPRLHHTISIRKMRETQDGFEFFSDQSYMDLAPSLRGNYPLHYVYSFQSGPYVYFLTVQREGGNSKAFHTRIVRMCSSDSEILRYVEMPFECIYSERRRKKRSAQVVFNVLQAAHVAKVGYDFQQEMGLKEGEDVLFAAFARSKPDSPEPTASSAVCLISITDINEFFKVFIQKGYTRKLHHFPGSEEKTFNQTFVGDSFSCGKHERGYRLEVTSTNPRRDYFHGRFRNVLLTSIAVVPIQNHTVVSLGTAEGRVIQVVVSRFGKTEPHVDFRLDTLPVSSEMALLSPQHHNGSLLLITGNKVSKLPVIGPGCEQLWTCSSCLLAPGFMGCGWCRTSNLCTRAPRCPQSQWIQDSCPLLITSISPSSAPLRGQTNITICGKNFGFNKKDRFDTKLIDVVVAGTKCKLERKDSNNNRLVCGLDHVNWSSVDSVVTVRSGKEQAQKDGFSFVNPVIIEIFPEFGPQSGGTMLTISGSFLDSGNVQTVTVGNATCVLQSVSATMLTCRTPPQPSPSQHKVQLHIDGVIFEAPVSYTYNKNPHISSVQPKHSFISGGSTVTVNGFYLHSALQPQMVLTAATEGKLFQVTCSHDEDKRNILCITPSLKGLSVQPPVATKMTFVLDGFSTDQYDLLYVEDPKFEEFQKPTVTPRGKKNILEIKVPPVNQEAVKNGEVLRVSNRTCESVTLVGNTLECTVPMELQTAAKELEVEWKQATSSVILGRVILAQDQDYRILITGGVCVSILLLLLIAVFVWIKRKKHINDQNLPLSQTESCRPHLYAHSHVDLSPMLGPMEGDLASPLLPSTAPIDLGSLHPELLKEVQHVVIAREDLLLHVNEIIGRGHFGCVFHGTLLEPDGQKQHCAIKSLNRITDIEEVSQFLKEGIIMKDFSHPNVLSLLGICLPSEGSPLVVLPYMKHGDLRNFIRDESHNPTVKDLMGFGLQVAKGMEYLASKKFVHRDLAARNCMLDESYTVKVADFGLARDVYDKEYYSVHNKHGVKLPVKWMALESLQTHKFTTKSDVWSFGVLLWELMTRGAPPYSDVNSFDITVFLLQGRRLLQPEFCPDALYNVMIECWHPKPERRPTFSELVSRISAIFSSFSGEHYILLNTTYVNIDKMTPYPSLISSQSNLDRDCCT

Target ORF information:

RefSeq Version	XM_005162995.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio MET proto-oncogene, receptor tyrosine kinase (met), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162995.4

ORF Insert Sequence:

ATGACAATTC ACTATTCTGA AGCTGCTTCC ATCCTAATCA TCCTTCAGTC GCTGTGGTGG 
GGCTTGAATT GTCAATGTGA GGAACCAATA GAAAGCTCCA AACTCGACCT CTCAGTGACC 
TATGACCTCC CTTACTTTGT GTCTGACACC CCCATTCAGA AGCTGTTGGA AATCAATGGA 
ACAGTGTATG TCGGTGCCGT CAATAGACTT TACGCTCTGT CGAAAGACCT GAAGAAGAAA 
CATGAGTATA AGACTGGACC GGTCCATGAG GGTCCAGACT GCAAGACCCC AACAGATCAA 
TGCAGTGGTT GTGAAAACAA GCCCCGTAAC ATAAACAACA CCAATATGGC CCTGTTAATG 
GAGACGTTCT ATGACCTGGA ACTTTTCAGC TGTGGCTCAG TCGGGAATGG CGTCTGCAGT 
CGTCATGTGT TAGAGGATGG GCCTCTGGGT GCGGAAGTAA CTTGCATGTA CACCAAAAAG 
AATGAAGGCA GCAGCCATGG ATGCCCAGAC TGCCTGGCTG GACCTGCGGG CACTCAGATC 
CTCAACATAA TGAGCGGTCG TGTTGTGAGG TTCTTCGTTG CGAACTCTGA ACCTCTTGAG 
TCAAACGGTC CACGTCTCCA CCACACTATT TCCATTAGGA AGATGCGTGA AACTCAAGAT 
GGCTTTGAGT TCTTTTCCGA TCAGTCCTAC ATGGATTTGG CCCCTTCACT GCGGGGGAAC 
TATCCACTAC ATTATGTCTA CTCTTTCCAG AGTGGTCCTT ATGTATATTT TCTCACCGTC 
CAACGCGAAG GTGGCAACTC GAAAGCTTTC CACACGAGAA TCGTACGCAT GTGTTCTTCA 
GATTCTGAGA TCCTGCGTTA TGTAGAAATG CCCTTTGAGT GCATTTACTC TGAGCGAAGG 
AGAAAAAAGC GTTCGGCTCA AGTGGTTTTC AACGTTCTCC AGGCTGCTCA TGTGGCCAAA 
GTTGGCTATG ACTTTCAGCA GGAGATGGGC TTGAAAGAAG GAGAGGACGT GCTGTTTGCT 
GCCTTTGCCC GGAGCAAACC GGACTCACCA GAGCCCACCG CCAGCTCCGC CGTTTGCCTT 
ATCTCCATCA CGGACATCAA TGAATTCTTC AAGGTTTTCA TTCAGAAGGG TTACACAAGG 
AAACTCCATC ACTTTCCAGG ATCTGAAGAG AAAACCTTCA ACCAGACGTT TGTAGGAGAT 
TCCTTCAGCT GTGGGAAACA TGAAAGAGGC TATCGGCTAG AAGTTACAAG CACCAACCCG 
CGCCGGGACT ATTTTCATGG CCGCTTCCGG AATGTTCTTC TCACTTCCAT AGCTGTGGTG 
CCGATCCAAA ACCACACTGT GGTCAGCCTT GGCACAGCTG AAGGCCGCGT CATCCAGGTG 
GTGGTTTCCC GCTTTGGCAA GACAGAGCCA CATGTGGACT TCCGCTTGGA CACGCTCCCT 
GTGTCTTCAG AAATGGCCCT GCTGTCTCCA CAGCATCACA ACGGCTCCTT ATTGTTGATA 
ACAGGAAACA AGGTCTCAAA GCTTCCTGTG ATCGGGCCTG GATGTGAGCA GTTGTGGACT 
TGTAGCTCGT GTCTTCTTGC TCCGGGCTTC ATGGGCTGTG GATGGTGCAG GACCAGCAAC 
CTGTGCACCA GGGCCCCTCG ATGCCCCCAG TCCCAATGGA TCCAGGACTC CTGCCCCCTC 
CTCATCACCT CGATCTCTCC TTCCTCCGCT CCACTCAGAG GTCAAACCAA CATCACAATC 
TGTGGCAAAA ACTTTGGCTT TAACAAAAAA GACAGATTTG ATACCAAACT GATAGACGTG 
GTGGTTGCTG GAACGAAGTG TAAATTGGAA AGGAAGGACA GTAACAATAA TCGGTTGGTC 
TGTGGACTGG ATCATGTGAA CTGGTCCAGC GTGGACTCTG TGGTCACAGT GCGCAGTGGC 
AAGGAACAGG CCCAGAAAGA TGGCTTCTCA TTTGTGAATC CAGTTATCAT AGAGATCTTC 
CCAGAGTTTG GACCTCAGTC TGGTGGGACA ATGCTCACTA TCAGCGGTTC ATTCTTGGAC 
AGTGGAAATG TGCAAACAGT CACAGTGGGG AACGCTACCT GTGTGCTGCA GAGTGTTTCA 
GCCACAATGT TAACATGTCG TACACCACCT CAGCCCTCGC CATCCCAACA CAAGGTACAG 
CTGCACATTG ATGGAGTAAT ATTTGAAGCG CCTGTCAGCT ACACCTACAA CAAGAACCCA 
CACATCTCCA GCGTCCAGCC CAAACATTCT TTCATCAGTG GAGGAAGCAC GGTGACAGTG 
AATGGCTTCT ACCTGCACTC AGCTCTTCAG CCTCAGATGG TTCTCACTGC TGCCACTGAG 
GGCAAACTCT TCCAAGTGAC CTGCAGTCAT GATGAGGATA AGAGAAATAT CCTTTGCATC 
ACGCCCTCCC TGAAAGGCCT CAGCGTTCAG CCTCCGGTCG CCACTAAAAT GACCTTCGTC 
CTGGATGGTT TTTCCACTGA TCAGTACGAC CTGCTGTACG TGGAAGATCC CAAATTTGAG 
GAGTTTCAGA AGCCCACTGT CACACCAAGG GGCAAAAAGA ACATTCTGGA GATTAAGGTC 
CCCCCTGTGA ATCAAGAGGC GGTGAAAAAC GGTGAGGTGC TGAGAGTTTC AAATCGGACC 
TGCGAGAGTG TCACTTTGGT GGGCAACACG CTCGAATGCA CCGTACCCAT GGAGCTCCAG 
ACCGCCGCCA AAGAGCTGGA GGTGGAGTGG AAGCAGGCCA CATCATCTGT GATCTTGGGC 
CGTGTGATTT TGGCTCAAGA CCAGGATTAC AGGATACTGA TCACTGGAGG AGTGTGTGTG 
TCCATCCTCC TCCTGCTCCT GATCGCTGTG TTTGTTTGGA TCAAGAGAAA GAAGCACATT 
AATGACCAAA ACTTGCCTCT GTCTCAGACA GAGTCCTGCC GGCCTCATCT CTACGCTCAT 
TCCCATGTGG ATCTGTCCCC AATGCTCGGG CCAATGGAAG GGGACCTGGC GTCTCCGCTG 
CTGCCCTCTA CAGCGCCTAT AGATCTAGGC AGCCTCCATC CTGAGCTGCT GAAGGAGGTC 
CAGCATGTGG TCATCGCAAG AGAAGATCTG CTCTTACATG TCAATGAGAT CATCGGGAGA 
GGGCACTTCG GCTGCGTGTT TCATGGAACC CTCCTCGAGC CAGATGGCCA GAAGCAGCAC 
TGCGCCATCA AGTCCTTAAA CCGAATCACA GATATCGAGG AGGTGTCTCA GTTTCTGAAG 
GAGGGCATCA TCATGAAGGA TTTCAGCCAT CCCAATGTGC TTTCTCTGCT GGGAATCTGC 
CTGCCCAGCG AGGGTTCGCC TCTCGTCGTG TTGCCTTACA TGAAGCACGG AGATCTGCGC 
AACTTTATCA GAGATGAAAG TCATAACCCC ACAGTGAAGG ACCTGATGGG TTTCGGGCTG 
CAGGTGGCTA AAGGAATGGA GTATCTCGCC AGCAAGAAAT TTGTTCACCG AGACCTCGCG 
GCCAGAAACT GCATGCTGGA TGAGAGCTAC ACAGTGAAGG TGGCAGATTT TGGCCTGGCC 
AGAGACGTGT ATGATAAAGA ATACTACAGC GTACACAACA AGCACGGAGT GAAGCTGCCT 
GTCAAATGGA TGGCGTTAGA AAGCCTGCAG ACACACAAGT TCACAACCAA ATCGGATGTG 
TGGTCGTTTG GTGTTTTGCT GTGGGAACTG ATGACCCGAG GTGCTCCGCC ATACTCTGAT 
GTGAACTCCT TCGACATTAC AGTGTTTCTT CTGCAAGGCC GGAGACTGTT ACAACCAGAG 
TTCTGCCCGG ATGCACTCTA TAATGTCATG ATTGAGTGCT GGCACCCCAA ACCCGAGCGT 
CGACCAACTT TCTCAGAACT AGTGTCTCGC ATCTCCGCCA TCTTCTCAAG CTTCAGCGGA 
GAGCACTACA TCCTCCTGAA CACCACCTAC GTCAACATCG ACAAAATGAC ACCCTACCCC 
TCTCTCATAT CATCTCAGAG CAACCTCGAC CGCGACTGCT GCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

met ( XM_005162995.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005163052.1 Danio rerio MET proto-oncogene, receptor tyrosine kinase (met), transcript variant X2, mRNA.

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CloneID	ODa29331
Clone ID Related Accession (Same CDS sequence)	XM_005162995.4 , XM_005162995.3
Accession Version	XM_005162995.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	hepatocyte growth factor receptor isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005162995.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAATTC ACTATTCTGA AGCTGCTTCC ATCCTAATCA TCCTTCAGTC GCTGTGGTGG 
GGCTTGAATT GTCAATGTGA GGAACCAATA GAAAGCTCCA AACTCGACCT CTCAGTGACC 
TATGACCTCC CTTACTTTGT GTCTGACACC CCCATTCAGA AGCTGTTGGA AATCAATGGA 
ACAGTGTATG TCGGTGCCGT CAATAGACTT TACGCTCTGT CGAAAGACCT GAAGAAGAAA 
CATGAGTATA AGACTGGACC GGTCCATGAG GGTCCAGACT GCAAGACCCC AACAGATCAA 
TGCAGTGGTT GTGAAAACAA GCCCCGTAAC ATAAACAACA CCAATATGGC CCTGTTAATG 
GAGACGTTCT ATGACCTGGA ACTTTTCAGC TGTGGCTCAG TCGGGAATGG CGTCTGCAGT 
CGTCATGTGT TAGAGGATGG GCCTCTGGGT GCGGAAGTAA CTTGCATGTA CACCAAAAAG 
AATGAAGGCA GCAGCCATGG ATGCCCAGAC TGCCTGGCTG GACCTGCGGG CACTCAGATC 
CTCAACATAA TGAGCGGTCG TGTTGTGAGG TTCTTCGTTG CGAACTCTGA ACCTCTTGAG 
TCAAACGGTC CACGTCTCCA CCACACTATT TCCATTAGGA AGATGCGTGA AACTCAAGAT 
GGCTTTGAGT TCTTTTCCGA TCAGTCCTAC ATGGATTTGG CCCCTTCACT GCGGGGGAAC 
TATCCACTAC ATTATGTCTA CTCTTTCCAG AGTGGTCCTT ATGTATATTT TCTCACCGTC 
CAACGCGAAG GTGGCAACTC GAAAGCTTTC CACACGAGAA TCGTACGCAT GTGTTCTTCA 
GATTCTGAGA TCCTGCGTTA TGTAGAAATG CCCTTTGAGT GCATTTACTC TGAGCGAAGG 
AGAAAAAAGC GTTCGGCTCA AGTGGTTTTC AACGTTCTCC AGGCTGCTCA TGTGGCCAAA 
GTTGGCTATG ACTTTCAGCA GGAGATGGGC TTGAAAGAAG GAGAGGACGT GCTGTTTGCT 
GCCTTTGCCC GGAGCAAACC GGACTCACCA GAGCCCACCG CCAGCTCCGC CGTTTGCCTT 
ATCTCCATCA CGGACATCAA TGAATTCTTC AAGGTTTTCA TTCAGAAGGG TTACACAAGG 
AAACTCCATC ACTTTCCAGG ATCTGAAGAG AAAACCTTCA ACCAGACGTT TGTAGGAGAT 
TCCTTCAGCT GTGGGAAACA TGAAAGAGGC TATCGGCTAG AAGTTACAAG CACCAACCCG 
CGCCGGGACT ATTTTCATGG CCGCTTCCGG AATGTTCTTC TCACTTCCAT AGCTGTGGTG 
CCGATCCAAA ACCACACTGT GGTCAGCCTT GGCACAGCTG AAGGCCGCGT CATCCAGGTG 
GTGGTTTCCC GCTTTGGCAA GACAGAGCCA CATGTGGACT TCCGCTTGGA CACGCTCCCT 
GTGTCTTCAG AAATGGCCCT GCTGTCTCCA CAGCATCACA ACGGCTCCTT ATTGTTGATA 
ACAGGAAACA AGGTCTCAAA GCTTCCTGTG ATCGGGCCTG GATGTGAGCA GTTGTGGACT 
TGTAGCTCGT GTCTTCTTGC TCCGGGCTTC ATGGGCTGTG GATGGTGCAG GACCAGCAAC 
CTGTGCACCA GGGCCCCTCG ATGCCCCCAG TCCCAATGGA TCCAGGACTC CTGCCCCCTC 
CTCATCACCT CGATCTCTCC TTCCTCCGCT CCACTCAGAG GTCAAACCAA CATCACAATC 
TGTGGCAAAA ACTTTGGCTT TAACAAAAAA GACAGATTTG ATACCAAACT GATAGACGTG 
GTGGTTGCTG GAACGAAGTG TAAATTGGAA AGGAAGGACA GTAACAATAA TCGGTTGGTC 
TGTGGACTGG ATCATGTGAA CTGGTCCAGC GTGGACTCTG TGGTCACAGT GCGCAGTGGC 
AAGGAACAGG CCCAGAAAGA TGGCTTCTCA TTTGTGAATC CAGTTATCAT AGAGATCTTC 
CCAGAGTTTG GACCTCAGTC TGGTGGGACA ATGCTCACTA TCAGCGGTTC ATTCTTGGAC 
AGTGGAAATG TGCAAACAGT CACAGTGGGG AACGCTACCT GTGTGCTGCA GAGTGTTTCA 
GCCACAATGT TAACATGTCG TACACCACCT CAGCCCTCGC CATCCCAACA CAAGGTACAG 
CTGCACATTG ATGGAGTAAT ATTTGAAGCG CCTGTCAGCT ACACCTACAA CAAGAACCCA 
CACATCTCCA GCGTCCAGCC CAAACATTCT TTCATCAGTG GAGGAAGCAC GGTGACAGTG 
AATGGCTTCT ACCTGCACTC AGCTCTTCAG CCTCAGATGG TTCTCACTGC TGCCACTGAG 
GGCAAACTCT TCCAAGTGAC CTGCAGTCAT GATGAGGATA AGAGAAATAT CCTTTGCATC 
ACGCCCTCCC TGAAAGGCCT CAGCGTTCAG CCTCCGGTCG CCACTAAAAT GACCTTCGTC 
CTGGATGGTT TTTCCACTGA TCAGTACGAC CTGCTGTACG TGGAAGATCC CAAATTTGAG 
GAGTTTCAGA AGCCCACTGT CACACCAAGG GGCAAAAAGA ACATTCTGGA GATTAAGGTC 
CCCCCTGTGA ATCAAGAGGC GGTGAAAAAC GGTGAGGTGC TGAGAGTTTC AAATCGGACC 
TGCGAGAGTG TCACTTTGGT GGGCAACACG CTCGAATGCA CCGTACCCAT GGAGCTCCAG 
ACCGCCGCCA AAGAGCTGGA GGTGGAGTGG AAGCAGGCCA CATCATCTGT GATCTTGGGC 
CGTGTGATTT TGGCTCAAGA CCAGGATTAC AGGATACTGA TCACTGGAGG AGTGTGTGTG 
TCCATCCTCC TCCTGCTCCT GATCGCTGTG TTTGTTTGGA TCAAGAGAAA GAAGCACATT 
AATGACCAAA ACTTGCCTCT GTCTCAGACA GAGTCCTGCC GGCCTCATCT CTACGCTCAT 
TCCCATGTGG ATCTGTCCCC AATGCTCGGG CCAATGGAAG GGGACCTGGC GTCTCCGCTG 
CTGCCCTCTA CAGCGCCTAT AGATCTAGGC AGCCTCCATC CTGAGCTGCT GAAGGAGGTC 
CAGCATGTGG TCATCGCAAG AGAAGATCTG CTCTTACATG TCAATGAGAT CATCGGGAGA 
GGGCACTTCG GCTGCGTGTT TCATGGAACC CTCCTCGAGC CAGATGGCCA GAAGCAGCAC 
TGCGCCATCA AGTCCTTAAA CCGAATCACA GATATCGAGG AGGTGTCTCA GTTTCTGAAG 
GAGGGCATCA TCATGAAGGA TTTCAGCCAT CCCAATGTGC TTTCTCTGCT GGGAATCTGC 
CTGCCCAGCG AGGGTTCGCC TCTCGTCGTG TTGCCTTACA TGAAGCACGG AGATCTGCGC 
AACTTTATCA GAGATGAAAG TCATAACCCC ACAGTGAAGG ACCTGATGGG TTTCGGGCTG 
CAGGTGGCTA AAGGAATGGA GTATCTCGCC AGCAAGAAAT TTGTTCACCG AGACCTCGCG 
GCCAGAAACT GCATGCTGGA TGAGAGCTAC ACAGTGAAGG TGGCAGATTT TGGCCTGGCC 
AGAGACGTGT ATGATAAAGA ATACTACAGC GTACACAACA AGCACGGAGT GAAGCTGCCT 
GTCAAATGGA TGGCGTTAGA AAGCCTGCAG ACACACAAGT TCACAACCAA ATCGGATGTG 
TGGTCGTTTG GTGTTTTGCT GTGGGAACTG ATGACCCGAG GTGCTCCGCC ATACTCTGAT 
GTGAACTCCT TCGACATTAC AGTGTTTCTT CTGCAAGGCC GGAGACTGTT ACAACCAGAG 
TTCTGCCCGG ATGCACTCTA TAATGTCATG ATTGAGTGCT GGCACCCCAA ACCCGAGCGT 
CGACCAACTT TCTCAGAACT AGTGTCTCGC ATCTCCGCCA TCTTCTCAAG CTTCAGCGGA 
GAGCACTACA TCCTCCTGAA CACCACCTAC GTCAACATCG ACAAAATGAC ACCCTACCCC 
TCTCTCATAT CATCTCAGAG CAACCTCGAC CGCGACTGCT GCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163052.1
CDS	343..4350
Translation	MTIHYSEAASILIILQSLWWGLNCQCEEPIESSKLDLSVTYDLPYFVSDTPIQKLLEINGTVYVGAVNRLYALSKDLKKKHEYKTGPVHEGPDCKTPTDQCSGCENKPRNINNTNMALLMETFYDLELFSCGSVGNGVCSRHVLEDGPLGAEVTCMYTKKNEGSSHGCPDCLAGPAGTQILNIMSGRVVRFFVANSEPLESNGPRLHHTISIRKMRETQDGFEFFSDQSYMDLAPSLRGNYPLHYVYSFQSGPYVYFLTVQREGGNSKAFHTRIVRMCSSDSEILRYVEMPFECIYSERRRKKRSAQVVFNVLQAAHVAKVGYDFQQEMGLKEGEDVLFAAFARSKPDSPEPTASSAVCLISITDINEFFKVFIQKGYTRKLHHFPGSEEKTFNQTFVGDSFSCGKHERGYRLEVTSTNPRRDYFHGRFRNVLLTSIAVVPIQNHTVVSLGTAEGRVIQVVVSRFGKTEPHVDFRLDTLPVSSEMALLSPQHHNGSLLLITGNKVSKLPVIGPGCEQLWTCSSCLLAPGFMGCGWCRTSNLCTRAPRCPQSQWIQDSCPLLITSISPSSAPLRGQTNITICGKNFGFNKKDRFDTKLIDVVVAGTKCKLERKDSNNNRLVCGLDHVNWSSVDSVVTVRSGKEQAQKDGFSFVNPVIIEIFPEFGPQSGGTMLTISGSFLDSGNVQTVTVGNATCVLQSVSATMLTCRTPPQPSPSQHKVQLHIDGVIFEAPVSYTYNKNPHISSVQPKHSFISGGSTVTVNGFYLHSALQPQMVLTAATEGKLFQVTCSHDEDKRNILCITPSLKGLSVQPPVATKMTFVLDGFSTDQYDLLYVEDPKFEEFQKPTVTPRGKKNILEIKVPPVNQEAVKNGEVLRVSNRTCESVTLVGNTLECTVPMELQTAAKELEVEWKQATSSVILGRVILAQDQDYRILITGGVCVSILLLLLIAVFVWIKRKKHINDQNLPLSQTESCRPHLYAHSHVDLSPMLGPMEGDLASPLLPSTAPIDLGSLHPELLKEVQHVVIAREDLLLHVNEIIGRGHFGCVFHGTLLEPDGQKQHCAIKSLNRITDIEEVSQFLKEGIIMKDFSHPNVLSLLGICLPSEGSPLVVLPYMKHGDLRNFIRDESHNPTVKDLMGFGLQVAKGMEYLASKKFVHRDLAARNCMLDESYTVKVADFGLARDVYDKEYYSVHNKHGVKLPVKWMALESLQTHKFTTKSDVWSFGVLLWELMTRGAPPYSDVNSFDITVFLLQGRRLLQPEFCPDALYNVMIECWHPKPERRPTFSELVSRISAIFSSFSGEHYILLNTTYVNIDKMTPYPSLISSQSNLDRDCCT

Target ORF information:

RefSeq Version	XM_005162995.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio MET proto-oncogene, receptor tyrosine kinase (met), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162995.3

ORF Insert Sequence:

ATGACAATTC ACTATTCTGA AGCTGCTTCC ATCCTAATCA TCCTTCAGTC GCTGTGGTGG 
GGCTTGAATT GTCAATGTGA GGAACCAATA GAAAGCTCCA AACTCGACCT CTCAGTGACC 
TATGACCTCC CTTACTTTGT GTCTGACACC CCCATTCAGA AGCTGTTGGA AATCAATGGA 
ACAGTGTATG TCGGTGCCGT CAATAGACTT TACGCTCTGT CGAAAGACCT GAAGAAGAAA 
CATGAGTATA AGACTGGACC GGTCCATGAG GGTCCAGACT GCAAGACCCC AACAGATCAA 
TGCAGTGGTT GTGAAAACAA GCCCCGTAAC ATAAACAACA CCAATATGGC CCTGTTAATG 
GAGACGTTCT ATGACCTGGA ACTTTTCAGC TGTGGCTCAG TCGGGAATGG CGTCTGCAGT 
CGTCATGTGT TAGAGGATGG GCCTCTGGGT GCGGAAGTAA CTTGCATGTA CACCAAAAAG 
AATGAAGGCA GCAGCCATGG ATGCCCAGAC TGCCTGGCTG GACCTGCGGG CACTCAGATC 
CTCAACATAA TGAGCGGTCG TGTTGTGAGG TTCTTCGTTG CGAACTCTGA ACCTCTTGAG 
TCAAACGGTC CACGTCTCCA CCACACTATT TCCATTAGGA AGATGCGTGA AACTCAAGAT 
GGCTTTGAGT TCTTTTCCGA TCAGTCCTAC ATGGATTTGG CCCCTTCACT GCGGGGGAAC 
TATCCACTAC ATTATGTCTA CTCTTTCCAG AGTGGTCCTT ATGTATATTT TCTCACCGTC 
CAACGCGAAG GTGGCAACTC GAAAGCTTTC CACACGAGAA TCGTACGCAT GTGTTCTTCA 
GATTCTGAGA TCCTGCGTTA TGTAGAAATG CCCTTTGAGT GCATTTACTC TGAGCGAAGG 
AGAAAAAAGC GTTCGGCTCA AGTGGTTTTC AACGTTCTCC AGGCTGCTCA TGTGGCCAAA 
GTTGGCTATG ACTTTCAGCA GGAGATGGGC TTGAAAGAAG GAGAGGACGT GCTGTTTGCT 
GCCTTTGCCC GGAGCAAACC GGACTCACCA GAGCCCACCG CCAGCTCCGC CGTTTGCCTT 
ATCTCCATCA CGGACATCAA TGAATTCTTC AAGGTTTTCA TTCAGAAGGG TTACACAAGG 
AAACTCCATC ACTTTCCAGG ATCTGAAGAG AAAACCTTCA ACCAGACGTT TGTAGGAGAT 
TCCTTCAGCT GTGGGAAACA TGAAAGAGGC TATCGGCTAG AAGTTACAAG CACCAACCCG 
CGCCGGGACT ATTTTCATGG CCGCTTCCGG AATGTTCTTC TCACTTCCAT AGCTGTGGTG 
CCGATCCAAA ACCACACTGT GGTCAGCCTT GGCACAGCTG AAGGCCGCGT CATCCAGGTG 
GTGGTTTCCC GCTTTGGCAA GACAGAGCCA CATGTGGACT TCCGCTTGGA CACGCTCCCT 
GTGTCTTCAG AAATGGCCCT GCTGTCTCCA CAGCATCACA ACGGCTCCTT ATTGTTGATA 
ACAGGAAACA AGGTCTCAAA GCTTCCTGTG ATCGGGCCTG GATGTGAGCA GTTGTGGACT 
TGTAGCTCGT GTCTTCTTGC TCCGGGCTTC ATGGGCTGTG GATGGTGCAG GACCAGCAAC 
CTGTGCACCA GGGCCCCTCG ATGCCCCCAG TCCCAATGGA TCCAGGACTC CTGCCCCCTC 
CTCATCACCT CGATCTCTCC TTCCTCCGCT CCACTCAGAG GTCAAACCAA CATCACAATC 
TGTGGCAAAA ACTTTGGCTT TAACAAAAAA GACAGATTTG ATACCAAACT GATAGACGTG 
GTGGTTGCTG GAACGAAGTG TAAATTGGAA AGGAAGGACA GTAACAATAA TCGGTTGGTC 
TGTGGACTGG ATCATGTGAA CTGGTCCAGC GTGGACTCTG TGGTCACAGT GCGCAGTGGC 
AAGGAACAGG CCCAGAAAGA TGGCTTCTCA TTTGTGAATC CAGTTATCAT AGAGATCTTC 
CCAGAGTTTG GACCTCAGTC TGGTGGGACA ATGCTCACTA TCAGCGGTTC ATTCTTGGAC 
AGTGGAAATG TGCAAACAGT CACAGTGGGG AACGCTACCT GTGTGCTGCA GAGTGTTTCA 
GCCACAATGT TAACATGTCG TACACCACCT CAGCCCTCGC CATCCCAACA CAAGGTACAG 
CTGCACATTG ATGGAGTAAT ATTTGAAGCG CCTGTCAGCT ACACCTACAA CAAGAACCCA 
CACATCTCCA GCGTCCAGCC CAAACATTCT TTCATCAGTG GAGGAAGCAC GGTGACAGTG 
AATGGCTTCT ACCTGCACTC AGCTCTTCAG CCTCAGATGG TTCTCACTGC TGCCACTGAG 
GGCAAACTCT TCCAAGTGAC CTGCAGTCAT GATGAGGATA AGAGAAATAT CCTTTGCATC 
ACGCCCTCCC TGAAAGGCCT CAGCGTTCAG CCTCCGGTCG CCACTAAAAT GACCTTCGTC 
CTGGATGGTT TTTCCACTGA TCAGTACGAC CTGCTGTACG TGGAAGATCC CAAATTTGAG 
GAGTTTCAGA AGCCCACTGT CACACCAAGG GGCAAAAAGA ACATTCTGGA GATTAAGGTC 
CCCCCTGTGA ATCAAGAGGC GGTGAAAAAC GGTGAGGTGC TGAGAGTTTC AAATCGGACC 
TGCGAGAGTG TCACTTTGGT GGGCAACACG CTCGAATGCA CCGTACCCAT GGAGCTCCAG 
ACCGCCGCCA AAGAGCTGGA GGTGGAGTGG AAGCAGGCCA CATCATCTGT GATCTTGGGC 
CGTGTGATTT TGGCTCAAGA CCAGGATTAC AGGATACTGA TCACTGGAGG AGTGTGTGTG 
TCCATCCTCC TCCTGCTCCT GATCGCTGTG TTTGTTTGGA TCAAGAGAAA GAAGCACATT 
AATGACCAAA ACTTGCCTCT GTCTCAGACA GAGTCCTGCC GGCCTCATCT CTACGCTCAT 
TCCCATGTGG ATCTGTCCCC AATGCTCGGG CCAATGGAAG GGGACCTGGC GTCTCCGCTG 
CTGCCCTCTA CAGCGCCTAT AGATCTAGGC AGCCTCCATC CTGAGCTGCT GAAGGAGGTC 
CAGCATGTGG TCATCGCAAG AGAAGATCTG CTCTTACATG TCAATGAGAT CATCGGGAGA 
GGGCACTTCG GCTGCGTGTT TCATGGAACC CTCCTCGAGC CAGATGGCCA GAAGCAGCAC 
TGCGCCATCA AGTCCTTAAA CCGAATCACA GATATCGAGG AGGTGTCTCA GTTTCTGAAG 
GAGGGCATCA TCATGAAGGA TTTCAGCCAT CCCAATGTGC TTTCTCTGCT GGGAATCTGC 
CTGCCCAGCG AGGGTTCGCC TCTCGTCGTG TTGCCTTACA TGAAGCACGG AGATCTGCGC 
AACTTTATCA GAGATGAAAG TCATAACCCC ACAGTGAAGG ACCTGATGGG TTTCGGGCTG 
CAGGTGGCTA AAGGAATGGA GTATCTCGCC AGCAAGAAAT TTGTTCACCG AGACCTCGCG 
GCCAGAAACT GCATGCTGGA TGAGAGCTAC ACAGTGAAGG TGGCAGATTT TGGCCTGGCC 
AGAGACGTGT ATGATAAAGA ATACTACAGC GTACACAACA AGCACGGAGT GAAGCTGCCT 
GTCAAATGGA TGGCGTTAGA AAGCCTGCAG ACACACAAGT TCACAACCAA ATCGGATGTG 
TGGTCGTTTG GTGTTTTGCT GTGGGAACTG ATGACCCGAG GTGCTCCGCC ATACTCTGAT 
GTGAACTCCT TCGACATTAC AGTGTTTCTT CTGCAAGGCC GGAGACTGTT ACAACCAGAG 
TTCTGCCCGG ATGCACTCTA TAATGTCATG ATTGAGTGCT GGCACCCCAA ACCCGAGCGT 
CGACCAACTT TCTCAGAACT AGTGTCTCGC ATCTCCGCCA TCTTCTCAAG CTTCAGCGGA 
GAGCACTACA TCCTCCTGAA CACCACCTAC GTCAACATCG ACAAAATGAC ACCCTACCCC 
TCTCTCATAT CATCTCAGAG CAACCTCGAC CGCGACTGCT GCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Maternal and embryonic exposure to the water soluble fraction of crude oil or lead induces behavioral abnormalities in zebrafish (Danio rerio), and the mechanisms involved.
Chemosphere1917-16(2018 Jan)
Wang Y,Shen C,Wang C,Zhou Y,Gao D,Zuo Z

Anti-angiogenic activity of water extract from Euphorbia pekinensis Rupr.
Journal of ethnopharmacology206337-346(2017 Jul)
Zhang W,Liu B,Feng Y,Liu J,Ma Z,Zheng J,Xia Q,Ni Y,Li F,Lin R

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met cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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