sphk2 cDNA ORF clone, Danio rerio(zebrafish)

The following sphk2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sphk2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa21674 XM_003200214.4
Latest version!
Danio rerio sphingosine kinase 2 (sphk2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $510.30
$729.00
ODa21674 XM_021466946.1
Latest version!
Danio rerio sphingosine kinase 2 (sphk2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $510.30
$729.00
ODa48844 XM_009292496.1
Latest version!
Danio rerio sphingosine kinase 2 (sphk2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $426.30
$609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa21674
    Clone ID Related Accession (Same CDS sequence) XM_003200214.4 , XM_021466946.1
    Accession Version XM_003200214.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2610bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product sphingosine kinase 2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_003200214.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCGTTCTC CTGGCCCAGA CAGCCCGTCC ATGACTGAGG AAGCATTGCT GCATGGACAG 
    TTTGGTGGTT GGAGTAGTGG AGGCGGCAGT GGAAGTAACA GCAACAGCTG CCCTAACAGT
    CCTGGAGGTG TGTCTTTACC TTCCAGCCCT GCCTCCACTA ACTACGCCTT GACCCTCACG
    CCAACTCACA TCCATGTCCA GCGGTTGTCT CCACGTGCAG GGAAGCAGTC CAGGTTGCTT
    CTGCCTATGG CAGAGCTGAC CGGATGCAGC TGCCCACGCT CACCAGCCCC TCCCTTACTA
    GTGCTTTACT GGTACCCTCC GGGACGCCGC CGGAAGGGTG TTTCTAGACA CAGACAGGTG
    AGGGCGTATT TGGCAGAGAG CCGAGCTGAG GCGGAGAAAT GGAACGCTGC TATACAGAGC
    CTGCTGAGGG GGATGGACGT CAGCTCCACT ACAGAATTTT GCAAAAGCAT GTTGCCTCGT
    CCCCGTCGAC TGTTGCTGCT GGTCAATCCA TTCAGTGGCC GGGGTCAGGC AATGCAATGG
    TGTCAAACGC ACATACTTCC AATGATAAGA GAAGCTAACA TCAGCTATAA CCTCATACAG
    ACAGAGCGTC AGAATCACGC ACGTGAGCTA ATCAGAGAGA TCTCACTACC AGAATGGGAT
    GGAATCGTCA TTGTGTCTGG AGATGGACTT TTGCATGAGG TGATAAATGG CCTTATGGAA
    AGGCCAGATT GGGAACAAGC AATAAAAACT CCTGTGGGAA TTCTGCCTTG TGGCTCTGGA
    AATGCACTTG CTGGTTCCAT CAACCACCAT GCTGGATATG ACATGTGCCT ACGGGAGCCT
    CTCCTCCTCA ACTGCTGTTT TCTGCTGTGC CGAGGTGGGG TTAAACCGTT GGACTTGGTC
    TCTGTTACAA CCAGCCCGTG TGCTTCATCA TCCACGTCTA ATCAAAATGG GCATCCACCT
    GCTCCCAGGA GGCTTTTCTC TTTCCTTTCT GTGGCTTGGG GTTTTGTTTC TGATGTGGAC
    ATAGAGAGCG AACGCTACAG AGGGCTGGGC TCAGCTCGTT TTACACTGGG AACACTAGTG
    AGATTGGCTT CTCTGCGCTC TTATAAGGGT CGCCTTTCCT ACCTGCCTCC TGCTGTAGTC
    AACCCGTCCC CTGATGCCAC ACCTCAGCCA CCCCGGAGGC CCCTGTCGCG CAGTATTACT
    GAAGGTCTGG AGGGATTTTG TCGCACACCC ATCCACCGCA CATGCTCTGA CATGGGCCTG
    AGTGAACAAA GAAGTCTCCG AAAAGATGAT GGAGAAAGAG AGAGGGAAAG AGAGCGGGAA
    AGACAGGAGA GAGAGCGGGA GAGGGAAAGG AGGAGAGACA GGGCAAGAGG GGTTGGGGTA
    GTGAGAGCCA GCAGTCTCGC AGAAGACCGG GAGAGAGAGG TAGAAGCAGA AGAGAAAATG
    GAGGAGATGT CTGGCACCAG CTCTGAGTCA ACTGAAAGGG ATGAATGTGA CAACATAGCC
    AAGAATAATG GTAACGAAGA AGAAAATAAT TCAGGAAAGA GTAAGATAGA TGGCCAAGAG
    TTGACAAATG CAAGAAATTT AAGTGACACA AATGAAGTAA ATGACTGTGA CGGAAGTTAT
    CAGACAACAC CACCTGAACT GCAACATGCA TTACGTAAAA ACTCGGCTCC CTCTAGCCAA
    ATAATTAATG CCTTCTTCAG CCAACCGATT GGTGCAGACA CTGACCAAGA CTTGGAGCTG
    GCAGCCTATG GCAAGGAGGA TGAGGACATA AATGGCACTT ATTTTCAGAA AGAGGCATAT
    TCATTGGACA AGTCTCGCGA GCGAGCCCTC ACTATCTCAT CCTCTCCATT CCGTCAGTCT
    CCTTTCAAAG CTTTCAAGCC CAAAACTTTG GACCAGAACC AGAACCCTTC AAGGCCAAGG
    CCCCTCTCTC TTCTCCACCA ATCCCATTCA AACTCTCTCC CTCCGAAATT TCCATCCCTT
    TCTCTGTCCC TTTCTCCAAC CCCTCCTTCA TCTCCATCTT GTGCCTCTCC ACACTCTTCT
    TATCTCGCAC CACGTCCCAA CACTCCCAGC TCATCTTCTC CATCCCCTTC TATTCATTCA
    CCAACTCCTT CCTTCAACTT TGACCTAGCA GAGCCTGCAG GACCCCTAAA AAACCGACCT
    CACATGACAT CCTCCATCAA CCTTCCAGAG GATGATTTGC TACCTCCTCT GGACCAGCCT
    CTCCCTACAA GGGACTGGGT TACTATTGAA GGTGATTTTG TCCTGGTTCT TGCCATCTAC
    CAGTCCCACC TGGGAGCAGA CCTTCTTGCT GCGCCTCAGG CTCGGTTTGA TGATGGCTTG
    ATCCATCTGA CATTTGTGCG GGCTGGCATC TCCCGAGCAA CACTACTTAG ACTGTTCCTA
    GCAATGGAGA GAGGAGCCCA TCTGTCTCTT AGCTCTCCCT ATGTTAGTCA CGTTTCTGCT
    CGGGCGTTCC GCCTTCAGCC ACTTTCCCCA CGAGGAACCC TTACTGTAGA TGGAGAGCTG
    GTGCCCTATG GGCCACTGCA GGCACAGGTT CATCCCTCCA TGGCTCGTTT GATTGTTGGG
    GACTCTGGAA TAAAGATTAC AAGATTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003200262.2
    CDS1245..3854
    Translation

    Target ORF information:

    RefSeq Version XM_003200214.4
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio sphingosine kinase 2 (sphk2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003200214.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCGTTCTC CTGGCCCAGA CAGCCCGTCC ATGACTGAGG AAGCATTGCT GCATGGACAG 
    TTTGGTGGTT GGAGTAGTGG AGGCGGCAGT GGAAGTAACA GCAACAGCTG CCCTAACAGT
    CCTGGAGGTG TGTCTTTACC TTCCAGCCCT GCCTCCACTA ACTACGCCTT GACCCTCACG
    CCAACTCACA TCCATGTCCA GCGGTTGTCT CCACGTGCAG GGAAGCAGTC CAGGTTGCTT
    CTGCCTATGG CAGAGCTGAC CGGATGCAGC TGCCCACGCT CACCAGCCCC TCCCTTACTA
    GTGCTTTACT GGTACCCTCC GGGACGCCGC CGGAAGGGTG TTTCTAGACA CAGACAGGTG
    AGGGCGTATT TGGCAGAGAG CCGAGCTGAG GCGGAGAAAT GGAACGCTGC TATACAGAGC
    CTGCTGAGGG GGATGGACGT CAGCTCCACT ACAGAATTTT GCAAAAGCAT GTTGCCTCGT
    CCCCGTCGAC TGTTGCTGCT GGTCAATCCA TTCAGTGGCC GGGGTCAGGC AATGCAATGG
    TGTCAAACGC ACATACTTCC AATGATAAGA GAAGCTAACA TCAGCTATAA CCTCATACAG
    ACAGAGCGTC AGAATCACGC ACGTGAGCTA ATCAGAGAGA TCTCACTACC AGAATGGGAT
    GGAATCGTCA TTGTGTCTGG AGATGGACTT TTGCATGAGG TGATAAATGG CCTTATGGAA
    AGGCCAGATT GGGAACAAGC AATAAAAACT CCTGTGGGAA TTCTGCCTTG TGGCTCTGGA
    AATGCACTTG CTGGTTCCAT CAACCACCAT GCTGGATATG ACATGTGCCT ACGGGAGCCT
    CTCCTCCTCA ACTGCTGTTT TCTGCTGTGC CGAGGTGGGG TTAAACCGTT GGACTTGGTC
    TCTGTTACAA CCAGCCCGTG TGCTTCATCA TCCACGTCTA ATCAAAATGG GCATCCACCT
    GCTCCCAGGA GGCTTTTCTC TTTCCTTTCT GTGGCTTGGG GTTTTGTTTC TGATGTGGAC
    ATAGAGAGCG AACGCTACAG AGGGCTGGGC TCAGCTCGTT TTACACTGGG AACACTAGTG
    AGATTGGCTT CTCTGCGCTC TTATAAGGGT CGCCTTTCCT ACCTGCCTCC TGCTGTAGTC
    AACCCGTCCC CTGATGCCAC ACCTCAGCCA CCCCGGAGGC CCCTGTCGCG CAGTATTACT
    GAAGGTCTGG AGGGATTTTG TCGCACACCC ATCCACCGCA CATGCTCTGA CATGGGCCTG
    AGTGAACAAA GAAGTCTCCG AAAAGATGAT GGAGAAAGAG AGAGGGAAAG AGAGCGGGAA
    AGACAGGAGA GAGAGCGGGA GAGGGAAAGG AGGAGAGACA GGGCAAGAGG GGTTGGGGTA
    GTGAGAGCCA GCAGTCTCGC AGAAGACCGG GAGAGAGAGG TAGAAGCAGA AGAGAAAATG
    GAGGAGATGT CTGGCACCAG CTCTGAGTCA ACTGAAAGGG ATGAATGTGA CAACATAGCC
    AAGAATAATG GTAACGAAGA AGAAAATAAT TCAGGAAAGA GTAAGATAGA TGGCCAAGAG
    TTGACAAATG CAAGAAATTT AAGTGACACA AATGAAGTAA ATGACTGTGA CGGAAGTTAT
    CAGACAACAC CACCTGAACT GCAACATGCA TTACGTAAAA ACTCGGCTCC CTCTAGCCAA
    ATAATTAATG CCTTCTTCAG CCAACCGATT GGTGCAGACA CTGACCAAGA CTTGGAGCTG
    GCAGCCTATG GCAAGGAGGA TGAGGACATA AATGGCACTT ATTTTCAGAA AGAGGCATAT
    TCATTGGACA AGTCTCGCGA GCGAGCCCTC ACTATCTCAT CCTCTCCATT CCGTCAGTCT
    CCTTTCAAAG CTTTCAAGCC CAAAACTTTG GACCAGAACC AGAACCCTTC AAGGCCAAGG
    CCCCTCTCTC TTCTCCACCA ATCCCATTCA AACTCTCTCC CTCCGAAATT TCCATCCCTT
    TCTCTGTCCC TTTCTCCAAC CCCTCCTTCA TCTCCATCTT GTGCCTCTCC ACACTCTTCT
    TATCTCGCAC CACGTCCCAA CACTCCCAGC TCATCTTCTC CATCCCCTTC TATTCATTCA
    CCAACTCCTT CCTTCAACTT TGACCTAGCA GAGCCTGCAG GACCCCTAAA AAACCGACCT
    CACATGACAT CCTCCATCAA CCTTCCAGAG GATGATTTGC TACCTCCTCT GGACCAGCCT
    CTCCCTACAA GGGACTGGGT TACTATTGAA GGTGATTTTG TCCTGGTTCT TGCCATCTAC
    CAGTCCCACC TGGGAGCAGA CCTTCTTGCT GCGCCTCAGG CTCGGTTTGA TGATGGCTTG
    ATCCATCTGA CATTTGTGCG GGCTGGCATC TCCCGAGCAA CACTACTTAG ACTGTTCCTA
    GCAATGGAGA GAGGAGCCCA TCTGTCTCTT AGCTCTCCCT ATGTTAGTCA CGTTTCTGCT
    CGGGCGTTCC GCCTTCAGCC ACTTTCCCCA CGAGGAACCC TTACTGTAGA TGGAGAGCTG
    GTGCCCTATG GGCCACTGCA GGCACAGGTT CATCCCTCCA TGGCTCGTTT GATTGTTGGG
    GACTCTGGAA TAAAGATTAC AAGATTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa21674
    Clone ID Related Accession (Same CDS sequence) XM_003200214.4 , XM_021466946.1
    Accession Version XM_021466946.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2610bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product sphingosine kinase 2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCGTTCTC CTGGCCCAGA CAGCCCGTCC ATGACTGAGG AAGCATTGCT GCATGGACAG 
    TTTGGTGGTT GGAGTAGTGG AGGCGGCAGT GGAAGTAACA GCAACAGCTG CCCTAACAGT
    CCTGGAGGTG TGTCTTTACC TTCCAGCCCT GCCTCCACTA ACTACGCCTT GACCCTCACG
    CCAACTCACA TCCATGTCCA GCGGTTGTCT CCACGTGCAG GGAAGCAGTC CAGGTTGCTT
    CTGCCTATGG CAGAGCTGAC CGGATGCAGC TGCCCACGCT CACCAGCCCC TCCCTTACTA
    GTGCTTTACT GGTACCCTCC GGGACGCCGC CGGAAGGGTG TTTCTAGACA CAGACAGGTG
    AGGGCGTATT TGGCAGAGAG CCGAGCTGAG GCGGAGAAAT GGAACGCTGC TATACAGAGC
    CTGCTGAGGG GGATGGACGT CAGCTCCACT ACAGAATTTT GCAAAAGCAT GTTGCCTCGT
    CCCCGTCGAC TGTTGCTGCT GGTCAATCCA TTCAGTGGCC GGGGTCAGGC AATGCAATGG
    TGTCAAACGC ACATACTTCC AATGATAAGA GAAGCTAACA TCAGCTATAA CCTCATACAG
    ACAGAGCGTC AGAATCACGC ACGTGAGCTA ATCAGAGAGA TCTCACTACC AGAATGGGAT
    GGAATCGTCA TTGTGTCTGG AGATGGACTT TTGCATGAGG TGATAAATGG CCTTATGGAA
    AGGCCAGATT GGGAACAAGC AATAAAAACT CCTGTGGGAA TTCTGCCTTG TGGCTCTGGA
    AATGCACTTG CTGGTTCCAT CAACCACCAT GCTGGATATG ACATGTGCCT ACGGGAGCCT
    CTCCTCCTCA ACTGCTGTTT TCTGCTGTGC CGAGGTGGGG TTAAACCGTT GGACTTGGTC
    TCTGTTACAA CCAGCCCGTG TGCTTCATCA TCCACGTCTA ATCAAAATGG GCATCCACCT
    GCTCCCAGGA GGCTTTTCTC TTTCCTTTCT GTGGCTTGGG GTTTTGTTTC TGATGTGGAC
    ATAGAGAGCG AACGCTACAG AGGGCTGGGC TCAGCTCGTT TTACACTGGG AACACTAGTG
    AGATTGGCTT CTCTGCGCTC TTATAAGGGT CGCCTTTCCT ACCTGCCTCC TGCTGTAGTC
    AACCCGTCCC CTGATGCCAC ACCTCAGCCA CCCCGGAGGC CCCTGTCGCG CAGTATTACT
    GAAGGTCTGG AGGGATTTTG TCGCACACCC ATCCACCGCA CATGCTCTGA CATGGGCCTG
    AGTGAACAAA GAAGTCTCCG AAAAGATGAT GGAGAAAGAG AGAGGGAAAG AGAGCGGGAA
    AGACAGGAGA GAGAGCGGGA GAGGGAAAGG AGGAGAGACA GGGCAAGAGG GGTTGGGGTA
    GTGAGAGCCA GCAGTCTCGC AGAAGACCGG GAGAGAGAGG TAGAAGCAGA AGAGAAAATG
    GAGGAGATGT CTGGCACCAG CTCTGAGTCA ACTGAAAGGG ATGAATGTGA CAACATAGCC
    AAGAATAATG GTAACGAAGA AGAAAATAAT TCAGGAAAGA GTAAGATAGA TGGCCAAGAG
    TTGACAAATG CAAGAAATTT AAGTGACACA AATGAAGTAA ATGACTGTGA CGGAAGTTAT
    CAGACAACAC CACCTGAACT GCAACATGCA TTACGTAAAA ACTCGGCTCC CTCTAGCCAA
    ATAATTAATG CCTTCTTCAG CCAACCGATT GGTGCAGACA CTGACCAAGA CTTGGAGCTG
    GCAGCCTATG GCAAGGAGGA TGAGGACATA AATGGCACTT ATTTTCAGAA AGAGGCATAT
    TCATTGGACA AGTCTCGCGA GCGAGCCCTC ACTATCTCAT CCTCTCCATT CCGTCAGTCT
    CCTTTCAAAG CTTTCAAGCC CAAAACTTTG GACCAGAACC AGAACCCTTC AAGGCCAAGG
    CCCCTCTCTC TTCTCCACCA ATCCCATTCA AACTCTCTCC CTCCGAAATT TCCATCCCTT
    TCTCTGTCCC TTTCTCCAAC CCCTCCTTCA TCTCCATCTT GTGCCTCTCC ACACTCTTCT
    TATCTCGCAC CACGTCCCAA CACTCCCAGC TCATCTTCTC CATCCCCTTC TATTCATTCA
    CCAACTCCTT CCTTCAACTT TGACCTAGCA GAGCCTGCAG GACCCCTAAA AAACCGACCT
    CACATGACAT CCTCCATCAA CCTTCCAGAG GATGATTTGC TACCTCCTCT GGACCAGCCT
    CTCCCTACAA GGGACTGGGT TACTATTGAA GGTGATTTTG TCCTGGTTCT TGCCATCTAC
    CAGTCCCACC TGGGAGCAGA CCTTCTTGCT GCGCCTCAGG CTCGGTTTGA TGATGGCTTG
    ATCCATCTGA CATTTGTGCG GGCTGGCATC TCCCGAGCAA CACTACTTAG ACTGTTCCTA
    GCAATGGAGA GAGGAGCCCA TCTGTCTCTT AGCTCTCCCT ATGTTAGTCA CGTTTCTGCT
    CGGGCGTTCC GCCTTCAGCC ACTTTCCCCA CGAGGAACCC TTACTGTAGA TGGAGAGCTG
    GTGCCCTATG GGCCACTGCA GGCACAGGTT CATCCCTCCA TGGCTCGTTT GATTGTTGGG
    GACTCTGGAA TAAAGATTAC AAGATTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322621.1
    CDS601..3210
    Translation

    Target ORF information:

    RefSeq Version XM_021466946.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio sphingosine kinase 2 (sphk2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021466946.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCGTTCTC CTGGCCCAGA CAGCCCGTCC ATGACTGAGG AAGCATTGCT GCATGGACAG 
    TTTGGTGGTT GGAGTAGTGG AGGCGGCAGT GGAAGTAACA GCAACAGCTG CCCTAACAGT
    CCTGGAGGTG TGTCTTTACC TTCCAGCCCT GCCTCCACTA ACTACGCCTT GACCCTCACG
    CCAACTCACA TCCATGTCCA GCGGTTGTCT CCACGTGCAG GGAAGCAGTC CAGGTTGCTT
    CTGCCTATGG CAGAGCTGAC CGGATGCAGC TGCCCACGCT CACCAGCCCC TCCCTTACTA
    GTGCTTTACT GGTACCCTCC GGGACGCCGC CGGAAGGGTG TTTCTAGACA CAGACAGGTG
    AGGGCGTATT TGGCAGAGAG CCGAGCTGAG GCGGAGAAAT GGAACGCTGC TATACAGAGC
    CTGCTGAGGG GGATGGACGT CAGCTCCACT ACAGAATTTT GCAAAAGCAT GTTGCCTCGT
    CCCCGTCGAC TGTTGCTGCT GGTCAATCCA TTCAGTGGCC GGGGTCAGGC AATGCAATGG
    TGTCAAACGC ACATACTTCC AATGATAAGA GAAGCTAACA TCAGCTATAA CCTCATACAG
    ACAGAGCGTC AGAATCACGC ACGTGAGCTA ATCAGAGAGA TCTCACTACC AGAATGGGAT
    GGAATCGTCA TTGTGTCTGG AGATGGACTT TTGCATGAGG TGATAAATGG CCTTATGGAA
    AGGCCAGATT GGGAACAAGC AATAAAAACT CCTGTGGGAA TTCTGCCTTG TGGCTCTGGA
    AATGCACTTG CTGGTTCCAT CAACCACCAT GCTGGATATG ACATGTGCCT ACGGGAGCCT
    CTCCTCCTCA ACTGCTGTTT TCTGCTGTGC CGAGGTGGGG TTAAACCGTT GGACTTGGTC
    TCTGTTACAA CCAGCCCGTG TGCTTCATCA TCCACGTCTA ATCAAAATGG GCATCCACCT
    GCTCCCAGGA GGCTTTTCTC TTTCCTTTCT GTGGCTTGGG GTTTTGTTTC TGATGTGGAC
    ATAGAGAGCG AACGCTACAG AGGGCTGGGC TCAGCTCGTT TTACACTGGG AACACTAGTG
    AGATTGGCTT CTCTGCGCTC TTATAAGGGT CGCCTTTCCT ACCTGCCTCC TGCTGTAGTC
    AACCCGTCCC CTGATGCCAC ACCTCAGCCA CCCCGGAGGC CCCTGTCGCG CAGTATTACT
    GAAGGTCTGG AGGGATTTTG TCGCACACCC ATCCACCGCA CATGCTCTGA CATGGGCCTG
    AGTGAACAAA GAAGTCTCCG AAAAGATGAT GGAGAAAGAG AGAGGGAAAG AGAGCGGGAA
    AGACAGGAGA GAGAGCGGGA GAGGGAAAGG AGGAGAGACA GGGCAAGAGG GGTTGGGGTA
    GTGAGAGCCA GCAGTCTCGC AGAAGACCGG GAGAGAGAGG TAGAAGCAGA AGAGAAAATG
    GAGGAGATGT CTGGCACCAG CTCTGAGTCA ACTGAAAGGG ATGAATGTGA CAACATAGCC
    AAGAATAATG GTAACGAAGA AGAAAATAAT TCAGGAAAGA GTAAGATAGA TGGCCAAGAG
    TTGACAAATG CAAGAAATTT AAGTGACACA AATGAAGTAA ATGACTGTGA CGGAAGTTAT
    CAGACAACAC CACCTGAACT GCAACATGCA TTACGTAAAA ACTCGGCTCC CTCTAGCCAA
    ATAATTAATG CCTTCTTCAG CCAACCGATT GGTGCAGACA CTGACCAAGA CTTGGAGCTG
    GCAGCCTATG GCAAGGAGGA TGAGGACATA AATGGCACTT ATTTTCAGAA AGAGGCATAT
    TCATTGGACA AGTCTCGCGA GCGAGCCCTC ACTATCTCAT CCTCTCCATT CCGTCAGTCT
    CCTTTCAAAG CTTTCAAGCC CAAAACTTTG GACCAGAACC AGAACCCTTC AAGGCCAAGG
    CCCCTCTCTC TTCTCCACCA ATCCCATTCA AACTCTCTCC CTCCGAAATT TCCATCCCTT
    TCTCTGTCCC TTTCTCCAAC CCCTCCTTCA TCTCCATCTT GTGCCTCTCC ACACTCTTCT
    TATCTCGCAC CACGTCCCAA CACTCCCAGC TCATCTTCTC CATCCCCTTC TATTCATTCA
    CCAACTCCTT CCTTCAACTT TGACCTAGCA GAGCCTGCAG GACCCCTAAA AAACCGACCT
    CACATGACAT CCTCCATCAA CCTTCCAGAG GATGATTTGC TACCTCCTCT GGACCAGCCT
    CTCCCTACAA GGGACTGGGT TACTATTGAA GGTGATTTTG TCCTGGTTCT TGCCATCTAC
    CAGTCCCACC TGGGAGCAGA CCTTCTTGCT GCGCCTCAGG CTCGGTTTGA TGATGGCTTG
    ATCCATCTGA CATTTGTGCG GGCTGGCATC TCCCGAGCAA CACTACTTAG ACTGTTCCTA
    GCAATGGAGA GAGGAGCCCA TCTGTCTCTT AGCTCTCCCT ATGTTAGTCA CGTTTCTGCT
    CGGGCGTTCC GCCTTCAGCC ACTTTCCCCA CGAGGAACCC TTACTGTAGA TGGAGAGCTG
    GTGCCCTATG GGCCACTGCA GGCACAGGTT CATCCCTCCA TGGCTCGTTT GATTGTTGGG
    GACTCTGGAA TAAAGATTAC AAGATTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48844
    Clone ID Related Accession (Same CDS sequence) XM_009292496.1
    Accession Version XM_009292496.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2142bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product sphingosine kinase 2 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTTGCCTC GTCCCCGTCG ACTGTTGCTG CTGGTCAATC CATTCAGTGG CCGGGGTCAG 
    GCAATGCAAT GGTGTCAAAC GCACATACTT CCAATGATAA GAGAAGCTAA CATCAGCTAT
    AACCTCATAC AGACAGAGCG TCAGAATCAC GCACGTGAGC TAATCAGAGA GATCTCACTA
    CCAGAATGGG ATGGAATCGT CATTGTGTCT GGAGATGGAC TTTTGCATGA GGTGATAAAT
    GGCCTTATGG AAAGGCCAGA TTGGGAACAA GCAATAAAAA CTCCTGTGGG AATTCTGCCT
    TGTGGCTCTG GAAATGCACT TGCTGGTTCC ATCAACCACC ATGCTGGATA TGACATGTGC
    CTACGGGAGC CTCTCCTCCT CAACTGCTGT TTTCTGCTGT GCCGAGGTGG GGTTAAACCG
    TTGGACTTGG TCTCTGTTAC AACCAGCCCG TGTGCTTCAT CATCCACGTC TAATCAAAAT
    GGGCATCCAC CTGCTCCCAG GAGGCTTTTC TCTTTCCTTT CTGTGGCTTG GGGTTTTGTT
    TCTGATGTGG ACATAGAGAG CGAACGCTAC AGAGGGCTGG GCTCAGCTCG TTTTACACTG
    GGAACACTAG TGAGATTGGC TTCTCTGCGC TCTTATAAGG GTCGCCTTTC CTACCTGCCT
    CCTGCTGTAG TCAACCCGTC CCCTGATGCC ACACCTCAGC CACCCCGGAG GCCCCTGTCG
    CGCAGTATTA CTGAAGGTCT GGAGGGATTT TGTCGCACAC CCATCCACCG CACATGCTCT
    GACATGGGCC TGAGTGAACA AAGAAGTCTC CGAAAAGATG ATGGAGAAAG AGAGAGGGAA
    AGAGAGCGGG AAAGACAGGA GAGAGAGCGG GAGAGGGAAA GGAGGAGAGA CAGGGCAAGA
    GGGGTTGGGG TAGTGAGAGC CAGCAGTCTC GCAGAAGACC GGGAGAGAGA GGTAGAAGCA
    GAAGAGAAAA TGGAGGAGAT GTCTGGCACC AGCTCTGAGT CAACTGAAAG GGATGAATGT
    GACAACATAG CCAAGAATAA TGGTAACGAA GAAGAAAATA ATTCAGGAAA GAGTAAGATA
    GATGGCCAAG AGTTGACAAA TGCAAGAAAT TTAAGTGACA CAAATGAAGT AAATGACTGT
    GACGGAAGTT ATCAGACAAC ACCACCTGAA CTGCAACATG CATTACGTAA AAACTCGGCT
    CCCTCTAGCC AAATAATTAA TGCCTTCTTC AGCCAACCGA TTGGTGCAGA CACTGACCAA
    GACTTGGAGC TGGCAGCCTA TGGCAAGGAG GATGAGGACA TAAATGGCAC TTATTTTCAG
    AAAGAGGCAT ATTCATTGGA CAAGTCTCGC GAGCGAGCCC TCACTATCTC ATCCTCTCCA
    TTCCGTCAGT CTCCTTTCAA AGCTTTCAAG CCCAAAACTT TGGACCAGAA CCAGAACCCT
    TCAAGGCCAA GGCCCCTCTC TCTTCTCCAC CAATCCCATT CAAACTCTCT CCCTCCGAAA
    TTTCCATCCC TTTCTCTGTC CCTTTCTCCA ACCCCTCCTT CATCTCCATC TTGTGCCTCT
    CCACACTCTT CTTATCTCGC ACCACGTCCC AACACTCCCA GCTCATCTTC TCCATCCCCT
    TCTATTCATT CACCAACTCC TTCCTTCAAC TTTGACCTAG CAGAGCCTGC AGGACCCCTA
    AAAAACCGAC CTCACATGAC ATCCTCCATC AACCTTCCAG AGGATGATTT GCTACCTCCT
    CTGGACCAGC CTCTCCCTAC AAGGGACTGG GTTACTATTG AAGGTGATTT TGTCCTGGTT
    CTTGCCATCT ACCAGTCCCA CCTGGGAGCA GACCTTCTTG CTGCGCCTCA GGCTCGGTTT
    GATGATGGCT TGATCCATCT GACATTTGTG CGGGCTGGCA TCTCCCGAGC AACACTACTT
    AGACTGTTCC TAGCAATGGA GAGAGGAGCC CATCTGTCTC TTAGCTCTCC CTATGTTAGT
    CACGTTTCTG CTCGGGCGTT CCGCCTTCAG CCACTTTCCC CACGAGGAAC CCTTACTGTA
    GATGGAGAGC TGGTGCCCTA TGGGCCACTG CAGGCACAGG TTCATCCCTC CATGGCTCGT
    TTGATTGTTG GGGACTCTGG AATAAAGATT ACAAGATTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290771.1
    CDS94..2235
    Translation

    Target ORF information:

    RefSeq Version XM_009292496.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio sphingosine kinase 2 (sphk2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292496.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTTGCCTC GTCCCCGTCG ACTGTTGCTG CTGGTCAATC CATTCAGTGG CCGGGGTCAG 
    GCAATGCAAT GGTGTCAAAC GCACATACTT CCAATGATAA GAGAAGCTAA CATCAGCTAT
    AACCTCATAC AGACAGAGCG TCAGAATCAC GCACGTGAGC TAATCAGAGA GATCTCACTA
    CCAGAATGGG ATGGAATCGT CATTGTGTCT GGAGATGGAC TTTTGCATGA GGTGATAAAT
    GGCCTTATGG AAAGGCCAGA TTGGGAACAA GCAATAAAAA CTCCTGTGGG AATTCTGCCT
    TGTGGCTCTG GAAATGCACT TGCTGGTTCC ATCAACCACC ATGCTGGATA TGACATGTGC
    CTACGGGAGC CTCTCCTCCT CAACTGCTGT TTTCTGCTGT GCCGAGGTGG GGTTAAACCG
    TTGGACTTGG TCTCTGTTAC AACCAGCCCG TGTGCTTCAT CATCCACGTC TAATCAAAAT
    GGGCATCCAC CTGCTCCCAG GAGGCTTTTC TCTTTCCTTT CTGTGGCTTG GGGTTTTGTT
    TCTGATGTGG ACATAGAGAG CGAACGCTAC AGAGGGCTGG GCTCAGCTCG TTTTACACTG
    GGAACACTAG TGAGATTGGC TTCTCTGCGC TCTTATAAGG GTCGCCTTTC CTACCTGCCT
    CCTGCTGTAG TCAACCCGTC CCCTGATGCC ACACCTCAGC CACCCCGGAG GCCCCTGTCG
    CGCAGTATTA CTGAAGGTCT GGAGGGATTT TGTCGCACAC CCATCCACCG CACATGCTCT
    GACATGGGCC TGAGTGAACA AAGAAGTCTC CGAAAAGATG ATGGAGAAAG AGAGAGGGAA
    AGAGAGCGGG AAAGACAGGA GAGAGAGCGG GAGAGGGAAA GGAGGAGAGA CAGGGCAAGA
    GGGGTTGGGG TAGTGAGAGC CAGCAGTCTC GCAGAAGACC GGGAGAGAGA GGTAGAAGCA
    GAAGAGAAAA TGGAGGAGAT GTCTGGCACC AGCTCTGAGT CAACTGAAAG GGATGAATGT
    GACAACATAG CCAAGAATAA TGGTAACGAA GAAGAAAATA ATTCAGGAAA GAGTAAGATA
    GATGGCCAAG AGTTGACAAA TGCAAGAAAT TTAAGTGACA CAAATGAAGT AAATGACTGT
    GACGGAAGTT ATCAGACAAC ACCACCTGAA CTGCAACATG CATTACGTAA AAACTCGGCT
    CCCTCTAGCC AAATAATTAA TGCCTTCTTC AGCCAACCGA TTGGTGCAGA CACTGACCAA
    GACTTGGAGC TGGCAGCCTA TGGCAAGGAG GATGAGGACA TAAATGGCAC TTATTTTCAG
    AAAGAGGCAT ATTCATTGGA CAAGTCTCGC GAGCGAGCCC TCACTATCTC ATCCTCTCCA
    TTCCGTCAGT CTCCTTTCAA AGCTTTCAAG CCCAAAACTT TGGACCAGAA CCAGAACCCT
    TCAAGGCCAA GGCCCCTCTC TCTTCTCCAC CAATCCCATT CAAACTCTCT CCCTCCGAAA
    TTTCCATCCC TTTCTCTGTC CCTTTCTCCA ACCCCTCCTT CATCTCCATC TTGTGCCTCT
    CCACACTCTT CTTATCTCGC ACCACGTCCC AACACTCCCA GCTCATCTTC TCCATCCCCT
    TCTATTCATT CACCAACTCC TTCCTTCAAC TTTGACCTAG CAGAGCCTGC AGGACCCCTA
    AAAAACCGAC CTCACATGAC ATCCTCCATC AACCTTCCAG AGGATGATTT GCTACCTCCT
    CTGGACCAGC CTCTCCCTAC AAGGGACTGG GTTACTATTG AAGGTGATTT TGTCCTGGTT
    CTTGCCATCT ACCAGTCCCA CCTGGGAGCA GACCTTCTTG CTGCGCCTCA GGCTCGGTTT
    GATGATGGCT TGATCCATCT GACATTTGTG CGGGCTGGCA TCTCCCGAGC AACACTACTT
    AGACTGTTCC TAGCAATGGA GAGAGGAGCC CATCTGTCTC TTAGCTCTCC CTATGTTAGT
    CACGTTTCTG CTCGGGCGTT CCGCCTTCAG CCACTTTCCC CACGAGGAAC CCTTACTGTA
    GATGGAGAGC TGGTGCCCTA TGGGCCACTG CAGGCACAGG TTCATCCCTC CATGGCTCGT
    TTGATTGTTG GGGACTCTGG AATAAAGATT ACAAGATTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.