ORF » Species Summary » Danio rerio » pycr1b cDNA ORF clone

pycr1b cDNA ORF clone, Danio rerio(zebrafish)

  • Gene

  • Clones

  • gRNAs

The following pycr1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pycr1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa48812 NM_001320425.1
Latest version!		 Danio rerio pyrroline-5-carboxylate reductase 1b (pycr1b), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
ODa48812 XM_021479539.1
Latest version!		 Danio rerio pyrroline-5-carboxylate reductase 1b (pycr1b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
ODa48812 XM_017358191.2
Latest version!		 Danio rerio pyrroline-5-carboxylate reductase 1b (pycr1b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
ODa147839 XM_021479538.1
Latest version!		 Danio rerio pyrroline-5-carboxylate reductase 1b (pycr1b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00
View Old Accession Versions >>
ODa48812 XM_017358191.1 Danio rerio pyrroline-5-carboxylate reductase 1b (pycr1b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    pycr1b ( NM_001320425.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001307354.1 Danio rerio pyrroline-5-carboxylate reductase 1b (pycr1b), mRNA.
    Price & Availability↑
    CloneID ODa48812
    Clone ID Related Accession (Same CDS sequence) XM_017358191.1 , NM_001320425.1 , XM_021479539.1 , XM_017358191.2
    Accession Version NM_001320425.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 954bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-26
    Organism Danio rerio(zebrafish)
    Product pyrroline-5-carboxylate reductase
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CABZ01074794.1, CN513194.1, EH479157.1 and FP325111.6. On Feb 25, 2016 this sequence version replaced XM_005169420.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: GFIL01044403.1, GDQH01019383.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAGTGTGG GCTTCATTGG AGCGGGTCAG CTGGCTCATG CCATGGTCAA GGGCTTCACC 
    GCAGCGGGTG TCATTGCAGC AAACAGAATC ACAGCGAGTT CACCAGACAC TGAACTGCCC 
    ACTGTCTCAG GCCTGCGGAA AATGGGGGTG AATTTGACCA CAAGCAACAA AGAAGCGACG 
    CACAGGAGCG ACGTTCTGTT TCTGGCCGTG AAGCCGCACA TCATCCCGTT TGTACTGGAC 
    GAGATCGGCC CCGATATTGA AGACCGACAC CTCATCGTGT CCTGCGCTGC TGGAGTCACT 
    ATCAGCTCCA TAGAGAAGAA GCTGCTGCAG TACCGTGAGT CTCCTAAAGT GATGCGATGC 
    ATGACGAACA CCCCGGTGGT GGTGCGCGAG GGGGCGACGG TGTACGCCAC AGGCACACAC 
    GCACATCTGG AGGACGGCAA ACTGCTGGAG CAGCTGATGG CCAGCGTGGG CTTCTGCACC 
    GAGGTGGAGG AGGACCTGAT CGATGCCGTC ACTGGACTCA GCGGCAGCGG ACCCGCATAT 
    GCATTCACGG CTCTGGATGC TCTTGCTGAT GGTGGGGTGA AGATGGGTTT GCCGAGGAGG 
    CTGGCTGTGC GTCTGGGGGC TCAGGCTCTA ATGGGAGCAG CGAAGATGTT GTTGGACAGT 
    GAGCAGCACC CGGGTCAGCT GAAGGATAAT GTGTGTTCTC CAGGTGGAGC TACAATACAC 
    GCGCTGCACT TCCTGGAGAG CGGAGGCTTC CGGAGCTTGC TGATCAACGC TGTGGAGGCG 
    TCCTGCATAC GCACAAGGGA GCTTCAGTCT CTGGCAGATC AGGAGCAGAT CTCCGCCGCA 
    GCCATCAAGA AGACCACGCT GGATAAAGTC CTGCAGCAGC CCGGCGTCAC GCACAACAGT 
    GTCGGCACCA GGAGCAGAGT AAACCTGTTC AACAACCGCA GCAAGAAGAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001307354.1
    CDS112..1065
    Translation

    Target ORF information:

    RefSeq Version NM_001320425.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio pyrroline-5-carboxylate reductase 1b (pycr1b), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001320425.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAGTGTGG GCTTCATTGG AGCGGGTCAG CTGGCTCATG CCATGGTCAA GGGCTTCACC 
    GCAGCGGGTG TCATTGCAGC AAACAGAATC ACAGCGAGTT CACCAGACAC TGAACTGCCC 
    ACTGTCTCAG GCCTGCGGAA AATGGGGGTG AATTTGACCA CAAGCAACAA AGAAGCGACG 
    CACAGGAGCG ACGTTCTGTT TCTGGCCGTG AAGCCGCACA TCATCCCGTT TGTACTGGAC 
    GAGATCGGCC CCGATATTGA AGACCGACAC CTCATCGTGT CCTGCGCTGC TGGAGTCACT 
    ATCAGCTCCA TAGAGAAGAA GCTGCTGCAG TACCGTGAGT CTCCTAAAGT GATGCGATGC 
    ATGACGAACA CCCCGGTGGT GGTGCGCGAG GGGGCGACGG TGTACGCCAC AGGCACACAC 
    GCACATCTGG AGGACGGCAA ACTGCTGGAG CAGCTGATGG CCAGCGTGGG CTTCTGCACC 
    GAGGTGGAGG AGGACCTGAT CGATGCCGTC ACTGGACTCA GCGGCAGCGG ACCCGCATAT 
    GCATTCACGG CTCTGGATGC TCTTGCTGAT GGTGGGGTGA AGATGGGTTT GCCGAGGAGG 
    CTGGCTGTGC GTCTGGGGGC TCAGGCTCTA ATGGGAGCAG CGAAGATGTT GTTGGACAGT 
    GAGCAGCACC CGGGTCAGCT GAAGGATAAT GTGTGTTCTC CAGGTGGAGC TACAATACAC 
    GCGCTGCACT TCCTGGAGAG CGGAGGCTTC CGGAGCTTGC TGATCAACGC TGTGGAGGCG 
    TCCTGCATAC GCACAAGGGA GCTTCAGTCT CTGGCAGATC AGGAGCAGAT CTCCGCCGCA 
    GCCATCAAGA AGACCACGCT GGATAAAGTC CTGCAGCAGC CCGGCGTCAC GCACAACAGT 
    GTCGGCACCA GGAGCAGAGT AAACCTGTTC AACAACCGCA GCAAGAAGAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    pycr1b ( XM_017358191.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213680.1 Danio rerio pyrroline-5-carboxylate reductase 1b (pycr1b), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID ODa48812
    Clone ID Related Accession (Same CDS sequence) XM_017358191.1 , NM_001320425.1 , XM_021479539.1 , XM_017358191.2
    Accession Version XM_017358191.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 954bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product pyrroline-5-carboxylate reductase isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAGTGTGG GCTTCATTGG AGCGGGTCAG CTGGCTCATG CCATGGTCAA GGGCTTCACC 
    GCAGCGGGTG TCATTGCAGC AAACAGAATC ACAGCGAGTT CACCAGACAC TGAACTGCCC 
    ACTGTCTCAG GCCTGCGGAA AATGGGGGTG AATTTGACCA CAAGCAACAA AGAAGCGACG 
    CACAGGAGCG ACGTTCTGTT TCTGGCCGTG AAGCCGCACA TCATCCCGTT TGTACTGGAC 
    GAGATCGGCC CCGATATTGA AGACCGACAC CTCATCGTGT CCTGCGCTGC TGGAGTCACT 
    ATCAGCTCCA TAGAGAAGAA GCTGCTGCAG TACCGTGAGT CTCCTAAAGT GATGCGATGC 
    ATGACGAACA CCCCGGTGGT GGTGCGCGAG GGGGCGACGG TGTACGCCAC AGGCACACAC 
    GCACATCTGG AGGACGGCAA ACTGCTGGAG CAGCTGATGG CCAGCGTGGG CTTCTGCACC 
    GAGGTGGAGG AGGACCTGAT CGATGCCGTC ACTGGACTCA GCGGCAGCGG ACCCGCATAT 
    GCATTCACGG CTCTGGATGC TCTTGCTGAT GGTGGGGTGA AGATGGGTTT GCCGAGGAGG 
    CTGGCTGTGC GTCTGGGGGC TCAGGCTCTA ATGGGAGCAG CGAAGATGTT GTTGGACAGT 
    GAGCAGCACC CGGGTCAGCT GAAGGATAAT GTGTGTTCTC CAGGTGGAGC TACAATACAC 
    GCGCTGCACT TCCTGGAGAG CGGAGGCTTC CGGAGCTTGC TGATCAACGC TGTGGAGGCG 
    TCCTGCATAC GCACAAGGGA GCTTCAGTCT CTGGCAGATC AGGAGCAGAT CTCCGCCGCA 
    GCCATCAAGA AGACCACGCT GGATAAAGTC CTGCAGCAGC CCGGCGTCAC GCACAACAGT 
    GTCGGCACCA GGAGCAGAGT AAACCTGTTC AACAACCGCA GCAAGAAGAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017213680.1
    CDS113..1066
    Translation

    Target ORF information:

    RefSeq Version XM_017358191.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio pyrroline-5-carboxylate reductase 1b (pycr1b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017358191.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAGTGTGG GCTTCATTGG AGCGGGTCAG CTGGCTCATG CCATGGTCAA GGGCTTCACC 
    GCAGCGGGTG TCATTGCAGC AAACAGAATC ACAGCGAGTT CACCAGACAC TGAACTGCCC 
    ACTGTCTCAG GCCTGCGGAA AATGGGGGTG AATTTGACCA CAAGCAACAA AGAAGCGACG 
    CACAGGAGCG ACGTTCTGTT TCTGGCCGTG AAGCCGCACA TCATCCCGTT TGTACTGGAC 
    GAGATCGGCC CCGATATTGA AGACCGACAC CTCATCGTGT CCTGCGCTGC TGGAGTCACT 
    ATCAGCTCCA TAGAGAAGAA GCTGCTGCAG TACCGTGAGT CTCCTAAAGT GATGCGATGC 
    ATGACGAACA CCCCGGTGGT GGTGCGCGAG GGGGCGACGG TGTACGCCAC AGGCACACAC 
    GCACATCTGG AGGACGGCAA ACTGCTGGAG CAGCTGATGG CCAGCGTGGG CTTCTGCACC 
    GAGGTGGAGG AGGACCTGAT CGATGCCGTC ACTGGACTCA GCGGCAGCGG ACCCGCATAT 
    GCATTCACGG CTCTGGATGC TCTTGCTGAT GGTGGGGTGA AGATGGGTTT GCCGAGGAGG 
    CTGGCTGTGC GTCTGGGGGC TCAGGCTCTA ATGGGAGCAG CGAAGATGTT GTTGGACAGT 
    GAGCAGCACC CGGGTCAGCT GAAGGATAAT GTGTGTTCTC CAGGTGGAGC TACAATACAC 
    GCGCTGCACT TCCTGGAGAG CGGAGGCTTC CGGAGCTTGC TGATCAACGC TGTGGAGGCG 
    TCCTGCATAC GCACAAGGGA GCTTCAGTCT CTGGCAGATC AGGAGCAGAT CTCCGCCGCA 
    GCCATCAAGA AGACCACGCT GGATAAAGTC CTGCAGCAGC CCGGCGTCAC GCACAACAGT 
    GTCGGCACCA GGAGCAGAGT AAACCTGTTC AACAACCGCA GCAAGAAGAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    pycr1b ( XM_021479539.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335214.1 Danio rerio pyrroline-5-carboxylate reductase 1b (pycr1b), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID ODa48812
    Clone ID Related Accession (Same CDS sequence) XM_017358191.1 , NM_001320425.1 , XM_021479539.1 , XM_017358191.2
    Accession Version XM_021479539.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 954bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product pyrroline-5-carboxylate reductase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAGTGTGG GCTTCATTGG AGCGGGTCAG CTGGCTCATG CCATGGTCAA GGGCTTCACC 
    GCAGCGGGTG TCATTGCAGC AAACAGAATC ACAGCGAGTT CACCAGACAC TGAACTGCCC 
    ACTGTCTCAG GCCTGCGGAA AATGGGGGTG AATTTGACCA CAAGCAACAA AGAAGCGACG 
    CACAGGAGCG ACGTTCTGTT TCTGGCCGTG AAGCCGCACA TCATCCCGTT TGTACTGGAC 
    GAGATCGGCC CCGATATTGA AGACCGACAC CTCATCGTGT CCTGCGCTGC TGGAGTCACT 
    ATCAGCTCCA TAGAGAAGAA GCTGCTGCAG TACCGTGAGT CTCCTAAAGT GATGCGATGC 
    ATGACGAACA CCCCGGTGGT GGTGCGCGAG GGGGCGACGG TGTACGCCAC AGGCACACAC 
    GCACATCTGG AGGACGGCAA ACTGCTGGAG CAGCTGATGG CCAGCGTGGG CTTCTGCACC 
    GAGGTGGAGG AGGACCTGAT CGATGCCGTC ACTGGACTCA GCGGCAGCGG ACCCGCATAT 
    GCATTCACGG CTCTGGATGC TCTTGCTGAT GGTGGGGTGA AGATGGGTTT GCCGAGGAGG 
    CTGGCTGTGC GTCTGGGGGC TCAGGCTCTA ATGGGAGCAG CGAAGATGTT GTTGGACAGT 
    GAGCAGCACC CGGGTCAGCT GAAGGATAAT GTGTGTTCTC CAGGTGGAGC TACAATACAC 
    GCGCTGCACT TCCTGGAGAG CGGAGGCTTC CGGAGCTTGC TGATCAACGC TGTGGAGGCG 
    TCCTGCATAC GCACAAGGGA GCTTCAGTCT CTGGCAGATC AGGAGCAGAT CTCCGCCGCA 
    GCCATCAAGA AGACCACGCT GGATAAAGTC CTGCAGCAGC CCGGCGTCAC GCACAACAGT 
    GTCGGCACCA GGAGCAGAGT AAACCTGTTC AACAACCGCA GCAAGAAGAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021335214.1
    CDS83..1036
    Translation

    Target ORF information:

    RefSeq Version XM_021479539.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio pyrroline-5-carboxylate reductase 1b (pycr1b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021479539.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAGTGTGG GCTTCATTGG AGCGGGTCAG CTGGCTCATG CCATGGTCAA GGGCTTCACC 
    GCAGCGGGTG TCATTGCAGC AAACAGAATC ACAGCGAGTT CACCAGACAC TGAACTGCCC 
    ACTGTCTCAG GCCTGCGGAA AATGGGGGTG AATTTGACCA CAAGCAACAA AGAAGCGACG 
    CACAGGAGCG ACGTTCTGTT TCTGGCCGTG AAGCCGCACA TCATCCCGTT TGTACTGGAC 
    GAGATCGGCC CCGATATTGA AGACCGACAC CTCATCGTGT CCTGCGCTGC TGGAGTCACT 
    ATCAGCTCCA TAGAGAAGAA GCTGCTGCAG TACCGTGAGT CTCCTAAAGT GATGCGATGC 
    ATGACGAACA CCCCGGTGGT GGTGCGCGAG GGGGCGACGG TGTACGCCAC AGGCACACAC 
    GCACATCTGG AGGACGGCAA ACTGCTGGAG CAGCTGATGG CCAGCGTGGG CTTCTGCACC 
    GAGGTGGAGG AGGACCTGAT CGATGCCGTC ACTGGACTCA GCGGCAGCGG ACCCGCATAT 
    GCATTCACGG CTCTGGATGC TCTTGCTGAT GGTGGGGTGA AGATGGGTTT GCCGAGGAGG 
    CTGGCTGTGC GTCTGGGGGC TCAGGCTCTA ATGGGAGCAG CGAAGATGTT GTTGGACAGT 
    GAGCAGCACC CGGGTCAGCT GAAGGATAAT GTGTGTTCTC CAGGTGGAGC TACAATACAC 
    GCGCTGCACT TCCTGGAGAG CGGAGGCTTC CGGAGCTTGC TGATCAACGC TGTGGAGGCG 
    TCCTGCATAC GCACAAGGGA GCTTCAGTCT CTGGCAGATC AGGAGCAGAT CTCCGCCGCA 
    GCCATCAAGA AGACCACGCT GGATAAAGTC CTGCAGCAGC CCGGCGTCAC GCACAACAGT 
    GTCGGCACCA GGAGCAGAGT AAACCTGTTC AACAACCGCA GCAAGAAGAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    pycr1b ( XM_017358191.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213680.1 Danio rerio pyrroline-5-carboxylate reductase 1b (pycr1b), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID ODa48812
    Clone ID Related Accession (Same CDS sequence) XM_017358191.1 , NM_001320425.1 , XM_021479539.1 , XM_017358191.2
    Accession Version XM_017358191.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 954bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product pyrroline-5-carboxylate reductase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017358191.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAGTGTGG GCTTCATTGG AGCGGGTCAG CTGGCTCATG CCATGGTCAA GGGCTTCACC 
    GCAGCGGGTG TCATTGCAGC AAACAGAATC ACAGCGAGTT CACCAGACAC TGAACTGCCC 
    ACTGTCTCAG GCCTGCGGAA AATGGGGGTG AATTTGACCA CAAGCAACAA AGAAGCGACG 
    CACAGGAGCG ACGTTCTGTT TCTGGCCGTG AAGCCGCACA TCATCCCGTT TGTACTGGAC 
    GAGATCGGCC CCGATATTGA AGACCGACAC CTCATCGTGT CCTGCGCTGC TGGAGTCACT 
    ATCAGCTCCA TAGAGAAGAA GCTGCTGCAG TACCGTGAGT CTCCTAAAGT GATGCGATGC 
    ATGACGAACA CCCCGGTGGT GGTGCGCGAG GGGGCGACGG TGTACGCCAC AGGCACACAC 
    GCACATCTGG AGGACGGCAA ACTGCTGGAG CAGCTGATGG CCAGCGTGGG CTTCTGCACC 
    GAGGTGGAGG AGGACCTGAT CGATGCCGTC ACTGGACTCA GCGGCAGCGG ACCCGCATAT 
    GCATTCACGG CTCTGGATGC TCTTGCTGAT GGTGGGGTGA AGATGGGTTT GCCGAGGAGG 
    CTGGCTGTGC GTCTGGGGGC TCAGGCTCTA ATGGGAGCAG CGAAGATGTT GTTGGACAGT 
    GAGCAGCACC CGGGTCAGCT GAAGGATAAT GTGTGTTCTC CAGGTGGAGC TACAATACAC 
    GCGCTGCACT TCCTGGAGAG CGGAGGCTTC CGGAGCTTGC TGATCAACGC TGTGGAGGCG 
    TCCTGCATAC GCACAAGGGA GCTTCAGTCT CTGGCAGATC AGGAGCAGAT CTCCGCCGCA 
    GCCATCAAGA AGACCACGCT GGATAAAGTC CTGCAGCAGC CCGGCGTCAC GCACAACAGT 
    GTCGGCACCA GGAGCAGAGT AAACCTGTTC AACAACCGCA GCAAGAAGAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017213680.1
    CDS141..1094
    Translation

    Target ORF information:

    RefSeq Version XM_017358191.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio pyrroline-5-carboxylate reductase 1b (pycr1b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017358191.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAGTGTGG GCTTCATTGG AGCGGGTCAG CTGGCTCATG CCATGGTCAA GGGCTTCACC 
    GCAGCGGGTG TCATTGCAGC AAACAGAATC ACAGCGAGTT CACCAGACAC TGAACTGCCC 
    ACTGTCTCAG GCCTGCGGAA AATGGGGGTG AATTTGACCA CAAGCAACAA AGAAGCGACG 
    CACAGGAGCG ACGTTCTGTT TCTGGCCGTG AAGCCGCACA TCATCCCGTT TGTACTGGAC 
    GAGATCGGCC CCGATATTGA AGACCGACAC CTCATCGTGT CCTGCGCTGC TGGAGTCACT 
    ATCAGCTCCA TAGAGAAGAA GCTGCTGCAG TACCGTGAGT CTCCTAAAGT GATGCGATGC 
    ATGACGAACA CCCCGGTGGT GGTGCGCGAG GGGGCGACGG TGTACGCCAC AGGCACACAC 
    GCACATCTGG AGGACGGCAA ACTGCTGGAG CAGCTGATGG CCAGCGTGGG CTTCTGCACC 
    GAGGTGGAGG AGGACCTGAT CGATGCCGTC ACTGGACTCA GCGGCAGCGG ACCCGCATAT 
    GCATTCACGG CTCTGGATGC TCTTGCTGAT GGTGGGGTGA AGATGGGTTT GCCGAGGAGG 
    CTGGCTGTGC GTCTGGGGGC TCAGGCTCTA ATGGGAGCAG CGAAGATGTT GTTGGACAGT 
    GAGCAGCACC CGGGTCAGCT GAAGGATAAT GTGTGTTCTC CAGGTGGAGC TACAATACAC 
    GCGCTGCACT TCCTGGAGAG CGGAGGCTTC CGGAGCTTGC TGATCAACGC TGTGGAGGCG 
    TCCTGCATAC GCACAAGGGA GCTTCAGTCT CTGGCAGATC AGGAGCAGAT CTCCGCCGCA 
    GCCATCAAGA AGACCACGCT GGATAAAGTC CTGCAGCAGC CCGGCGTCAC GCACAACAGT 
    GTCGGCACCA GGAGCAGAGT AAACCTGTTC AACAACCGCA GCAAGAAGAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    pycr1b ( XM_021479538.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335213.1 Danio rerio pyrroline-5-carboxylate reductase 1b (pycr1b), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID ODa147839
    Clone ID Related Accession (Same CDS sequence) XM_021479538.1
    Accession Version XM_021479538.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1089bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product pyrroline-5-carboxylate reductase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGATTGGCC GGCTCGTGTG TCAGTCAGAG CGCAGAGCTG CTGCACATGG AAGCGCTGGA 
    GCCACGTTGA CATCACTGGT GTTTCTACAG CTTCTCCAAC AACAATCTCA AGTTCAAGTT 
    CAGTCTCGGC GCAGGATGAG TGTGGGCTTC ATTGGAGCGG GTCAGCTGGC TCATGCCATG 
    GTCAAGGGCT TCACCGCAGC GGGTGTCATT GCAGCAAACA GAATCACAGC GAGTTCACCA 
    GACACTGAAC TGCCCACTGT CTCAGGCCTG CGGAAAATGG GGGTGAATTT GACCACAAGC 
    AACAAAGAAG CGACGCACAG GAGCGACGTT CTGTTTCTGG CCGTGAAGCC GCACATCATC 
    CCGTTTGTAC TGGACGAGAT CGGCCCCGAT ATTGAAGACC GACACCTCAT CGTGTCCTGC 
    GCTGCTGGAG TCACTATCAG CTCCATAGAG AAGAAGCTGC TGCAGTACCG TGAGTCTCCT 
    AAAGTGATGC GATGCATGAC GAACACCCCG GTGGTGGTGC GCGAGGGGGC GACGGTGTAC 
    GCCACAGGCA CACACGCACA TCTGGAGGAC GGCAAACTGC TGGAGCAGCT GATGGCCAGC 
    GTGGGCTTCT GCACCGAGGT GGAGGAGGAC CTGATCGATG CCGTCACTGG ACTCAGCGGC 
    AGCGGACCCG CATATGCATT CACGGCTCTG GATGCTCTTG CTGATGGTGG GGTGAAGATG 
    GGTTTGCCGA GGAGGCTGGC TGTGCGTCTG GGGGCTCAGG CTCTAATGGG AGCAGCGAAG 
    ATGTTGTTGG ACAGTGAGCA GCACCCGGGT CAGCTGAAGG ATAATGTGTG TTCTCCAGGT 
    GGAGCTACAA TACACGCGCT GCACTTCCTG GAGAGCGGAG GCTTCCGGAG CTTGCTGATC 
    AACGCTGTGG AGGCGTCCTG CATACGCACA AGGGAGCTTC AGTCTCTGGC AGATCAGGAG 
    CAGATCTCCG CCGCAGCCAT CAAGAAGACC ACGCTGGATA AAGTCCTGCA GCAGCCCGGC 
    GTCACGCACA ACAGTGTCGG CACCAGGAGC AGAGTAAACC TGTTCAACAA CCGCAGCAAG 
    AAGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021335213.1
    CDS12..1100
    Translation

    Target ORF information:

    RefSeq Version XM_021479538.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio pyrroline-5-carboxylate reductase 1b (pycr1b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021479538.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGATTGGCC GGCTCGTGTG TCAGTCAGAG CGCAGAGCTG CTGCACATGG AAGCGCTGGA 
    GCCACGTTGA CATCACTGGT GTTTCTACAG CTTCTCCAAC AACAATCTCA AGTTCAAGTT 
    CAGTCTCGGC GCAGGATGAG TGTGGGCTTC ATTGGAGCGG GTCAGCTGGC TCATGCCATG 
    GTCAAGGGCT TCACCGCAGC GGGTGTCATT GCAGCAAACA GAATCACAGC GAGTTCACCA 
    GACACTGAAC TGCCCACTGT CTCAGGCCTG CGGAAAATGG GGGTGAATTT GACCACAAGC 
    AACAAAGAAG CGACGCACAG GAGCGACGTT CTGTTTCTGG CCGTGAAGCC GCACATCATC 
    CCGTTTGTAC TGGACGAGAT CGGCCCCGAT ATTGAAGACC GACACCTCAT CGTGTCCTGC 
    GCTGCTGGAG TCACTATCAG CTCCATAGAG AAGAAGCTGC TGCAGTACCG TGAGTCTCCT 
    AAAGTGATGC GATGCATGAC GAACACCCCG GTGGTGGTGC GCGAGGGGGC GACGGTGTAC 
    GCCACAGGCA CACACGCACA TCTGGAGGAC GGCAAACTGC TGGAGCAGCT GATGGCCAGC 
    GTGGGCTTCT GCACCGAGGT GGAGGAGGAC CTGATCGATG CCGTCACTGG ACTCAGCGGC 
    AGCGGACCCG CATATGCATT CACGGCTCTG GATGCTCTTG CTGATGGTGG GGTGAAGATG 
    GGTTTGCCGA GGAGGCTGGC TGTGCGTCTG GGGGCTCAGG CTCTAATGGG AGCAGCGAAG 
    ATGTTGTTGG ACAGTGAGCA GCACCCGGGT CAGCTGAAGG ATAATGTGTG TTCTCCAGGT 
    GGAGCTACAA TACACGCGCT GCACTTCCTG GAGAGCGGAG GCTTCCGGAG CTTGCTGATC 
    AACGCTGTGG AGGCGTCCTG CATACGCACA AGGGAGCTTC AGTCTCTGGC AGATCAGGAG 
    CAGATCTCCG CCGCAGCCAT CAAGAAGACC ACGCTGGATA AAGTCCTGCA GCAGCCCGGC 
    GTCACGCACA ACAGTGTCGG CACCAGGAGC AGAGTAAACC TGTTCAACAA CCGCAGCAAG 
    AAGAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Evolution of complexity in the zebrafish synapse proteome.
    Nature communications814613(2017 Mar)
    Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG


    Mutations in PYCR2, Encoding Pyrroline-5-Carboxylate Reductase 2, Cause Microcephaly and Hypomyelination.
    American journal of human genetics96(5)709-19(2015 May)
    Nakayama T,Al-Maawali A,El-Quessny M,Rajab A,Khalil S,Stoler JM,Tan WH,Nasir R,Schmitz-Abe K,Hill RS,Partlow JN,Al-Saffar M,Servattalab S,LaCoursiere CM,Tambunan DE,Coulter ME,Elhosary PC,Gorski G,Barkovich AJ,Markianos K,Poduri A,Mochida GH


    Mutations in PYCR1 cause cutis laxa with progeroid features.
    Nature genetics41(9)1016-21(2009 Sep)
    Reversade B,Escande-Beillard N,Dimopoulou A,Fischer B,Chng SC,Li Y,Shboul M,Tham PY,Kayserili H,Al-Gazali L,Shahwan M,Brancati F,Lee H,O'Connor BD,Schmidt-von Kegler M,Merriman B,Nelson SF,Masri A,Alkazaleh F,Guerra D,Ferrari P,Nanda A,Rajab A,Markie D,Gray M,Nelson J,Grix A,Sommer A,Savarirayan R,Janecke AR,Steichen E,Sillence D,Hausser I,Budde B,N?rnberg G,N?rnberg P,Seemann P,Kunkel D,Zambruno G,Dallapiccola B,Schuelke M,Robertson S,Hamamy H,Wollnik B,Van Maldergem L,Mundlos S,Kornak U


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