col12a1a cDNA ORF clone, Danio rerio(zebrafish)

The following col12a1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the col12a1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa48774 XM_009293298.3
Latest version!
Danio rerio collagen, type XII, alpha 1a (col12a1a), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa22912 XM_002665259.6
Latest version!
Danio rerio collagen, type XII, alpha 1a (col12a1a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa48772 XM_009293296.3
Latest version!
Danio rerio collagen, type XII, alpha 1a (col12a1a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa48773 XM_009293297.3
Latest version!
Danio rerio collagen, type XII, alpha 1a (col12a1a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
View Old Accession Versions >>
ODa48773 XM_009293297.2 Danio rerio collagen, type XII, alpha 1a (col12a1a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa22912 XM_002665259.5 Danio rerio collagen, type XII, alpha 1a (col12a1a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa48774 XM_009293298.2 Danio rerio collagen, type XII, alpha 1a (col12a1a), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa48772 XM_009293296.2 Danio rerio collagen, type XII, alpha 1a (col12a1a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa48774
    Clone ID Related Accession (Same CDS sequence) XM_009293298.3 , XM_009293298.2
    Accession Version XM_009293298.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5853bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product collagen alpha-1(XII) chain isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293298.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGAAGATCA GGTTGTCTTT GGCCGCTGCT GCGCTCTTGA CTCTAGTACT CAGTTCAGTA 
    GAGGCTCAAG ATGCCCAGTG TAAAACCACG GCCCAAGCGG ATATCGTCCT GCTTGTTGAT
    GGATCTTGGA GCATTGGTCG CCTGAACTTC AAGACCATAC GCAATTTCAT TGCTCGCATG
    GTGGGAGTCT TCGACATCGG CCCCGATCGT GTCCAGATTG GTCTGGCACA GTACAGCGGA
    GACCCGAAGA CTGAGTGGCA TCTCAATGCT CACAGAACCA GGACTCAGCT GCTGGATGCG
    GTGGCCAATC TACCCTACAA AGGAGGAAAC ACTCTCACCG GCCTTGCTTT GAATTACATC
    CTTCAGAATA ACTTCAAGCC TAACGTTGGC ATGCGTCCAA ACTCCAGAAA GATCGGCGTC
    CTGGTCACTG ATGGCAAGTC CCAAGACGAC ATTGTGGCGA ACTCACAGAA CCTGCGGGAC
    CAAGGGATTG AACTGTACGC CATTGGTGTA AAGAACGCAG ATGAGAATGA GTTGAGAACC
    ATTGCTTCCG ATCCCGATGA CATTCACATG TACAACGTGG CTGATTTCTC CTTCCTGCTG
    GACATTGTGG ATGACCTCAC AAACAACCTG TGCAACAGCG TCAAAGGTCC AGGAGGAGGA
    CCAGAGCCAC CCACTGATCT GACCACCTCA GAGGTCACAC ATTACTCTTT CCGTGCCACT
    TGGGTGGCTC CTGACGGGCC TGTGGAGAAG TACAGAGTGG AGTATGTGAG CACCGCTGGA
    GGAGAACCAC AGCAACAGAT GTTTGTGGAC GGGACGGTGA CCACTGTGGT TTTGGAAGGT
    CTCACTCCGC TGACCGAGTA CATAGTAAAG GTTTACTCAG TGGTGGGAGA GGACAGCAGC
    GACCCACTCA GAGGCATAGA GACCACATTG CCTCTCAGTG CTGTGAGGAG CATGAACATC
    TACAAAGAGC GCTCCACCAC CATGAATGTG AGATGGGACG CAGCCACAGG GGCCACAGGC
    TACATGCTTC TCTACAAGGC CCTTAACGCC ACTGAACCCT CTGTGGAGCA GGAGATGCGA
    GTGAAAGGAA TGGTCACTGA CGTCCAGCTG AAGAAACTTC TCCCCAACAC GGCCTACAGT
    GTTTCGCTCT TCGCCCTGCA CGGCGATTAC GCCAGTGAAC CTCTGATTGA TCAAGGGGTG
    ACCCTGCCTC TTCCTCCATC CGGTGAGTTG AGGATTACCG ATGTGACCCA CAGCACCATG
    CAGCTGGACT GGGACGCTGC GCCCGGCGCC GTCCGAAAGT ACATCATCAC TTACAAGCCA
    GAGGAGGGAG AGGCGAAGGA GCTTGAAGTT GACGGAGATG TAACATCTAG AGGCCTGGAT
    AATCTCATCT CCCAGACTGA ATATGATGTG GCTGTGACTC CGGTTTATGA TTCGGGCACT
    GGAAACCCCA TGCTTAACCA GGCCATTACA GATGTGGTTC CTGCCCCGCA GAACTTGAGG
    TTCTCGATGG TGACCCAGAC CAGTTTTAGG GTTCACTGGG AGCACGGCGC TCCTGATGTC
    TCCCTCTACC GTATTGGCTG GAAAAAGAAA GGAACGACCG AGTTTCAAAA TGATATCCTG
    AGTAGTGAAG ACACTACATA TGATCTGGTG GACCTGGAGC CAGACACACC TTATGATGTT
    TCGGTCACTG CCATTTACCC TGATGAGTCT GAGAGCGAGG ATCTGCTGGG CACTGAACGC
    ACTTTGGCCA AGCTTGCTAC ACCACCTCCC AGTGGTCCTC CTCAAAACCT CCAGGTGTTC
    GACGAGACCA CCACCTCCCT CACGACTAAG TGGGACCACG CGCCTGGACG AGTTCAGAAC
    TACAAAATCA CCTACGTACC GCTTACTGGA GGACGATCAC AGTCGACGCA GGTCGGAGGA
    AAGAAAAACA CAGTGATCCT GCAGAAACTG ACTCCAGACA CTCCTTACTC CATCACTGTT
    GCTGCGGTCT ACGCCAGCGG AGAGGCCAGA GACATTTCTG GCCAAGGAAA GACATTGCCG
    CTGGGCAGTG TCAGGAATCT TCAGGTCACT GACCCCACAG TTAGCACTCT GAATGTGCGC
    TGGGAGCCGG CGGAGGGAAG CGTTCGCCAG TACAGGATCT ACTACCAGCC CGTCGCAGGG
    GGCGCAGAGG ACATGGTCCA GGTTCCTGGA AGCACCACCA GCACCGTTCT GAAGAACCTG
    CAATCTGACA CTCAGTACAG AGTGACGGTG GTGCCAGTCT ACGCTCCTGG AGAGGGCAAG
    CGCATGTCGG AGACCGGAAA AACACTGGTT CGGACCCCTG CCAAGAACAT CCAGGTTTAC
    AACCCCACCA CAAACACCCT GAACGTGCGC TGGGAGCCGG CCTCAGGACA GGTGCAGCAG
    TACCGCGTGG CTTACGCCCC TCTCTCCGGG ATCCGACCCT CTCAGTCGGT GCTGGTTCCA
    GGAAACACCC ACACGGCTCT GCTGCAGCAG CTGGAATCAG ACACTGACTA CTCTGTGAGT
    GTGGTGGCTC TGTATGCTGA TGGAGAGGGC TCGTCTGTGT CTGATAATGG CAAAACACTG
    CCCCGCAGTG GCCCCAGGAA CCTGCGGGTT TATGATCCCA CCACCAGCAC GCTGTCGGTG
    GGCTGGGAAC ATGCAGAAGG GCCTGTGATT CAGTACCGCA TTGGCTACGC ACCCACCACT
    GGAGATCCCA TTGAAGAATT TACAACTGTC CCAGGCAACC GTAACTCGGT CATCCTGCAA
    AACCTGCAGC CAGACACGCC GTACAACATC AACGTAGAAG CCATTTATCA AGATGGCCCT
    GGAGGACGAC TCAGTGGAGA TGGAAAAACA TTGGATCTCC TTGGGCCTCG TAACCTGCGT
    GTTTCAGACG AGTGGTACAC ACGTTTCCGA GTGTCCTGGG ATCCTGCAGC CGCACGGGTG
    AATGGATATA AACTGGTCTA TACACCAGTG GGCACAACCG ACACCATGGA AGCCTTCGTT
    GGAGACGTGA CTTCACACCA CCTGAACAAC CTCCAGCCTG GCACCACATA CGACCTGAAG
    GTTTACGCTC AGTATGATGC GGGCTTGAGC GGCGCTCTGG TCGGACAGGG AACCACCCTG
    TATCTCAACG TGACCGACCT CACTTCCTAC AACGTGGGCT ACGACTCTTT CTGCATCAGA
    TGGACTCCTC ATCGAGCTGC CACCTCCTAC AGACTCAAAG TCAACCCTTT CAACCCATCT
    CACCGTGGAG CTCAGGAGGT CACGATCACT GCGGCTGAGA GCCAGTACTG TTTTGACGGC
    CTGACGCCAG ACGCGCTATA CAACGCCACC GTCTACACGC AGACCCCCAA CCGAGAGGGG
    CCCGGAGTCA GTGTCAAAGA GCGCACACTG GTGAAGCCCA CCGTCGCCCC AACACTACCT
    CCAACTCCTC CACCTCCAGC CACCATTCCT TCTGCACTCG AGGTGTGCAA AGGTGCCAAG
    GCTGACCTGG TGTTCCTGAT CGATGGCTCG TGGAGTATCG GAGATGACAG CTTCGCTAAA
    GTCCGGCAGT TTGTGTTCAG CATGATTGGG GCGTTTGATG TGGTCAGCCA TGAGGGCATG
    CAGGTGTCTT TCGTGCAGTA CAGCGACGAC GCCAAAACTG AGTTCAAGCT CAACACCTAC
    CACGACAAGG GAATGGTGCT GTCTGCTCTT CAGATGGTCA ATTATAGAGG AGGAAACACC
    AAAACAGGTG CTGCACTGAA GCATGTTGGA GAAAAAGTGT TCACCTCGGA CAATGGCATG
    AGGAGAACCG TTCCCAAGGT ACTGGTTGTG GTGACCGATG GACGTTCTCA AGATGACGTG
    AAGAAGAGCG CTGCTAAACT ACAGCATGCA GGTTACAGTG TGTTTGTGGT GGGTGTCGCT
    GACATTGACG TTAATGAACT GAGGAACATT GGAAGCAAGC CCAGTGACAG ACACGTCTTT
    ATCGTCGACA ACTTCGATGC TTTCGCCAAG ATCCAAGACA ACCTGATCAC GTTCATCTGT
    GAAACGGCAA CGTCCACATG CCCTTTGATT TACCTCAATG GATTCACATC ACCAGGCTTC
    AGGATGCTGG AAGCTTTCAA CATCACTGAC AAGACCTACG CAGGCATGAA CGGCGTCTCT
    CTGGAAACCG GCTCCTTCAA CAGCTTCATC GCATACAGAC TCCATAAGGA CGCCTTCATT
    TCCCAGCCAA CAAAGGAAAT CCATCCAGAT GGTCTTCCTC CATCCTACAC CCTCATCCTC
    CTCTTCCGTC TGCTCCCGGA CACCGGCAAG GATGCGTTTG ATTTATGGCA GATCTCAGAT
    AAGAACAATA ACCCTGAGGT CGGGCTCACC ATTGACCCCT CCAGCAGAGT GATCTCCTTC
    TACAATAAAG ACACTAGAGG GGAAATTCAG AAAGTCTCCT TTGATAACGA CCACGTGAAG
    AAGATTTTCC ACGGCAGTTT CCACAAGCTT CACATTTCTA TTTCGCCGAA AAATGTCAAA
    CTCAACATCG ACTGCCAAGA GGTGGCTGAG AAACAGATCA AAGCAGCCAA CAACGTCTCC
    ACAGATGGCT ACGAAGTCCT TGGAAAAATG ACAAAGTCTG GCGGTTCAAA GAAGCAGTCG
    GCCACATTCC AGCTCCAGAT GTTTGATATT GTCTGCAGTT TGGGCTGGAC AAGTCGAGAC
    AAATGTTGTG ATATTCCAGC AATGCGAGAT GAAGCCAAGT GTCCTGCTCT TCCTCACTCC
    TGCACATGTG CTCAGGACAG TGTTGGTCCT CCAGGCCCAG CGGGTCCTCA AGGCAGTCCT
    GGTACAAAAG GCCCACGAGG CGAGAGAGGA GAGTCTGGTC CTACGGGTCC AATGGGTCCA
    CGTGGAGAGC AGGGTCTGCC AGGGCAGATG GGGCCACCGG GTCCACAGGG CCCCAATGGA
    CTATCCCTTC CCGGAGAACC AGGGCGACCG GGACCTAAGG GAGACCCCGG TGACTCAGGT
    TTACCTGGAC AGAGAGGCCC GGTTGGCGCT GCAGGTCCAC TCGGCCCTGT TGGACCCGCT
    GGAGCAAGAG GTCCACCAGG AAGTGAAGGC CCATCTGGAC CAAGAGGTCC ATCGGGACCA
    ATGGGACCAC CGGGAGCTCC CGGAATGCCA GGAATCACCG GAAAACCAGG AAAACCCGGA
    GACACTGGCT TTCCTGGACC TGTTGGCATG AAAGGAGAGA AGGGTGAAAG AGGAGACACT
    GCGTCACAGA ATATGATGCG CTCTGTAGCG AGACAAGTCT GCGAACAGCT AGTCAGCAGA
    CAAATGAGCA GAATTGATAT GATGATGAAC CAGATCCCCA GCAACTACAG GAGTAACAGC
    CCCGGCCCCG CTGGACCTCC AGGCCCACCT GGAAACCAGG GACCCCATGG AGAGCCTGGT
    CAGCCCGGAC CCAATGGCTT CCCAGGAAAT CCAGGTTTAC CAGGCTCTCC TGGAGAAAGA
    GGAGTGCCTG GAGAGAAGGG TGAACGAGGC AGCCCCGGCA TTGGCACTCG AGGACAGAGA
    GGATTACCTG GACCTCCTGG GCCACCAGGG CAGTCTCAGA CCGGTCCCCC TGGTCCCTCA
    GGCTCACCCG GGCCCCGAGG ACCACCAGGA CAACATGGTT CTTCTGGAGT CACAGGACCA
    CCAGGACCTC CAGGATACTG TGACTCCTCT CAGTGCGGCA TGCCTTACAA CGGACAAGGC
    TACCCAGGCC CCTACCAGCC CGGGTCTGAA ACCTACGTAG TGCCCATACC AGCCGCGCCA
    TCGGAAGATA TCGAGACACA GTCGCCAGGA TTGCGCTACC AGCGCGGAAA AAGGTCGCTA
    TCAAGAAAGG AAGCCGAAAG AATCAAAACA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009291573.1
    CDS178..6030
    Translation

    Target ORF information:

    RefSeq Version XM_009293298.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio collagen, type XII, alpha 1a (col12a1a), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009293298.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGAAGATCA GGTTGTCTTT GGCCGCTGCT GCGCTCTTGA CTCTAGTACT CAGTTCAGTA 
    GAGGCTCAAG ATGCCCAGTG TAAAACCACG GCCCAAGCGG ATATCGTCCT GCTTGTTGAT
    GGATCTTGGA GCATTGGTCG CCTGAACTTC AAGACCATAC GCAATTTCAT TGCTCGCATG
    GTGGGAGTCT TCGACATCGG CCCCGATCGT GTCCAGATTG GTCTGGCACA GTACAGCGGA
    GACCCGAAGA CTGAGTGGCA TCTCAATGCT CACAGAACCA GGACTCAGCT GCTGGATGCG
    GTGGCCAATC TACCCTACAA AGGAGGAAAC ACTCTCACCG GCCTTGCTTT GAATTACATC
    CTTCAGAATA ACTTCAAGCC TAACGTTGGC ATGCGTCCAA ACTCCAGAAA GATCGGCGTC
    CTGGTCACTG ATGGCAAGTC CCAAGACGAC ATTGTGGCGA ACTCACAGAA CCTGCGGGAC
    CAAGGGATTG AACTGTACGC CATTGGTGTA AAGAACGCAG ATGAGAATGA GTTGAGAACC
    ATTGCTTCCG ATCCCGATGA CATTCACATG TACAACGTGG CTGATTTCTC CTTCCTGCTG
    GACATTGTGG ATGACCTCAC AAACAACCTG TGCAACAGCG TCAAAGGTCC AGGAGGAGGA
    CCAGAGCCAC CCACTGATCT GACCACCTCA GAGGTCACAC ATTACTCTTT CCGTGCCACT
    TGGGTGGCTC CTGACGGGCC TGTGGAGAAG TACAGAGTGG AGTATGTGAG CACCGCTGGA
    GGAGAACCAC AGCAACAGAT GTTTGTGGAC GGGACGGTGA CCACTGTGGT TTTGGAAGGT
    CTCACTCCGC TGACCGAGTA CATAGTAAAG GTTTACTCAG TGGTGGGAGA GGACAGCAGC
    GACCCACTCA GAGGCATAGA GACCACATTG CCTCTCAGTG CTGTGAGGAG CATGAACATC
    TACAAAGAGC GCTCCACCAC CATGAATGTG AGATGGGACG CAGCCACAGG GGCCACAGGC
    TACATGCTTC TCTACAAGGC CCTTAACGCC ACTGAACCCT CTGTGGAGCA GGAGATGCGA
    GTGAAAGGAA TGGTCACTGA CGTCCAGCTG AAGAAACTTC TCCCCAACAC GGCCTACAGT
    GTTTCGCTCT TCGCCCTGCA CGGCGATTAC GCCAGTGAAC CTCTGATTGA TCAAGGGGTG
    ACCCTGCCTC TTCCTCCATC CGGTGAGTTG AGGATTACCG ATGTGACCCA CAGCACCATG
    CAGCTGGACT GGGACGCTGC GCCCGGCGCC GTCCGAAAGT ACATCATCAC TTACAAGCCA
    GAGGAGGGAG AGGCGAAGGA GCTTGAAGTT GACGGAGATG TAACATCTAG AGGCCTGGAT
    AATCTCATCT CCCAGACTGA ATATGATGTG GCTGTGACTC CGGTTTATGA TTCGGGCACT
    GGAAACCCCA TGCTTAACCA GGCCATTACA GATGTGGTTC CTGCCCCGCA GAACTTGAGG
    TTCTCGATGG TGACCCAGAC CAGTTTTAGG GTTCACTGGG AGCACGGCGC TCCTGATGTC
    TCCCTCTACC GTATTGGCTG GAAAAAGAAA GGAACGACCG AGTTTCAAAA TGATATCCTG
    AGTAGTGAAG ACACTACATA TGATCTGGTG GACCTGGAGC CAGACACACC TTATGATGTT
    TCGGTCACTG CCATTTACCC TGATGAGTCT GAGAGCGAGG ATCTGCTGGG CACTGAACGC
    ACTTTGGCCA AGCTTGCTAC ACCACCTCCC AGTGGTCCTC CTCAAAACCT CCAGGTGTTC
    GACGAGACCA CCACCTCCCT CACGACTAAG TGGGACCACG CGCCTGGACG AGTTCAGAAC
    TACAAAATCA CCTACGTACC GCTTACTGGA GGACGATCAC AGTCGACGCA GGTCGGAGGA
    AAGAAAAACA CAGTGATCCT GCAGAAACTG ACTCCAGACA CTCCTTACTC CATCACTGTT
    GCTGCGGTCT ACGCCAGCGG AGAGGCCAGA GACATTTCTG GCCAAGGAAA GACATTGCCG
    CTGGGCAGTG TCAGGAATCT TCAGGTCACT GACCCCACAG TTAGCACTCT GAATGTGCGC
    TGGGAGCCGG CGGAGGGAAG CGTTCGCCAG TACAGGATCT ACTACCAGCC CGTCGCAGGG
    GGCGCAGAGG ACATGGTCCA GGTTCCTGGA AGCACCACCA GCACCGTTCT GAAGAACCTG
    CAATCTGACA CTCAGTACAG AGTGACGGTG GTGCCAGTCT ACGCTCCTGG AGAGGGCAAG
    CGCATGTCGG AGACCGGAAA AACACTGGTT CGGACCCCTG CCAAGAACAT CCAGGTTTAC
    AACCCCACCA CAAACACCCT GAACGTGCGC TGGGAGCCGG CCTCAGGACA GGTGCAGCAG
    TACCGCGTGG CTTACGCCCC TCTCTCCGGG ATCCGACCCT CTCAGTCGGT GCTGGTTCCA
    GGAAACACCC ACACGGCTCT GCTGCAGCAG CTGGAATCAG ACACTGACTA CTCTGTGAGT
    GTGGTGGCTC TGTATGCTGA TGGAGAGGGC TCGTCTGTGT CTGATAATGG CAAAACACTG
    CCCCGCAGTG GCCCCAGGAA CCTGCGGGTT TATGATCCCA CCACCAGCAC GCTGTCGGTG
    GGCTGGGAAC ATGCAGAAGG GCCTGTGATT CAGTACCGCA TTGGCTACGC ACCCACCACT
    GGAGATCCCA TTGAAGAATT TACAACTGTC CCAGGCAACC GTAACTCGGT CATCCTGCAA
    AACCTGCAGC CAGACACGCC GTACAACATC AACGTAGAAG CCATTTATCA AGATGGCCCT
    GGAGGACGAC TCAGTGGAGA TGGAAAAACA TTGGATCTCC TTGGGCCTCG TAACCTGCGT
    GTTTCAGACG AGTGGTACAC ACGTTTCCGA GTGTCCTGGG ATCCTGCAGC CGCACGGGTG
    AATGGATATA AACTGGTCTA TACACCAGTG GGCACAACCG ACACCATGGA AGCCTTCGTT
    GGAGACGTGA CTTCACACCA CCTGAACAAC CTCCAGCCTG GCACCACATA CGACCTGAAG
    GTTTACGCTC AGTATGATGC GGGCTTGAGC GGCGCTCTGG TCGGACAGGG AACCACCCTG
    TATCTCAACG TGACCGACCT CACTTCCTAC AACGTGGGCT ACGACTCTTT CTGCATCAGA
    TGGACTCCTC ATCGAGCTGC CACCTCCTAC AGACTCAAAG TCAACCCTTT CAACCCATCT
    CACCGTGGAG CTCAGGAGGT CACGATCACT GCGGCTGAGA GCCAGTACTG TTTTGACGGC
    CTGACGCCAG ACGCGCTATA CAACGCCACC GTCTACACGC AGACCCCCAA CCGAGAGGGG
    CCCGGAGTCA GTGTCAAAGA GCGCACACTG GTGAAGCCCA CCGTCGCCCC AACACTACCT
    CCAACTCCTC CACCTCCAGC CACCATTCCT TCTGCACTCG AGGTGTGCAA AGGTGCCAAG
    GCTGACCTGG TGTTCCTGAT CGATGGCTCG TGGAGTATCG GAGATGACAG CTTCGCTAAA
    GTCCGGCAGT TTGTGTTCAG CATGATTGGG GCGTTTGATG TGGTCAGCCA TGAGGGCATG
    CAGGTGTCTT TCGTGCAGTA CAGCGACGAC GCCAAAACTG AGTTCAAGCT CAACACCTAC
    CACGACAAGG GAATGGTGCT GTCTGCTCTT CAGATGGTCA ATTATAGAGG AGGAAACACC
    AAAACAGGTG CTGCACTGAA GCATGTTGGA GAAAAAGTGT TCACCTCGGA CAATGGCATG
    AGGAGAACCG TTCCCAAGGT ACTGGTTGTG GTGACCGATG GACGTTCTCA AGATGACGTG
    AAGAAGAGCG CTGCTAAACT ACAGCATGCA GGTTACAGTG TGTTTGTGGT GGGTGTCGCT
    GACATTGACG TTAATGAACT GAGGAACATT GGAAGCAAGC CCAGTGACAG ACACGTCTTT
    ATCGTCGACA ACTTCGATGC TTTCGCCAAG ATCCAAGACA ACCTGATCAC GTTCATCTGT
    GAAACGGCAA CGTCCACATG CCCTTTGATT TACCTCAATG GATTCACATC ACCAGGCTTC
    AGGATGCTGG AAGCTTTCAA CATCACTGAC AAGACCTACG CAGGCATGAA CGGCGTCTCT
    CTGGAAACCG GCTCCTTCAA CAGCTTCATC GCATACAGAC TCCATAAGGA CGCCTTCATT
    TCCCAGCCAA CAAAGGAAAT CCATCCAGAT GGTCTTCCTC CATCCTACAC CCTCATCCTC
    CTCTTCCGTC TGCTCCCGGA CACCGGCAAG GATGCGTTTG ATTTATGGCA GATCTCAGAT
    AAGAACAATA ACCCTGAGGT CGGGCTCACC ATTGACCCCT CCAGCAGAGT GATCTCCTTC
    TACAATAAAG ACACTAGAGG GGAAATTCAG AAAGTCTCCT TTGATAACGA CCACGTGAAG
    AAGATTTTCC ACGGCAGTTT CCACAAGCTT CACATTTCTA TTTCGCCGAA AAATGTCAAA
    CTCAACATCG ACTGCCAAGA GGTGGCTGAG AAACAGATCA AAGCAGCCAA CAACGTCTCC
    ACAGATGGCT ACGAAGTCCT TGGAAAAATG ACAAAGTCTG GCGGTTCAAA GAAGCAGTCG
    GCCACATTCC AGCTCCAGAT GTTTGATATT GTCTGCAGTT TGGGCTGGAC AAGTCGAGAC
    AAATGTTGTG ATATTCCAGC AATGCGAGAT GAAGCCAAGT GTCCTGCTCT TCCTCACTCC
    TGCACATGTG CTCAGGACAG TGTTGGTCCT CCAGGCCCAG CGGGTCCTCA AGGCAGTCCT
    GGTACAAAAG GCCCACGAGG CGAGAGAGGA GAGTCTGGTC CTACGGGTCC AATGGGTCCA
    CGTGGAGAGC AGGGTCTGCC AGGGCAGATG GGGCCACCGG GTCCACAGGG CCCCAATGGA
    CTATCCCTTC CCGGAGAACC AGGGCGACCG GGACCTAAGG GAGACCCCGG TGACTCAGGT
    TTACCTGGAC AGAGAGGCCC GGTTGGCGCT GCAGGTCCAC TCGGCCCTGT TGGACCCGCT
    GGAGCAAGAG GTCCACCAGG AAGTGAAGGC CCATCTGGAC CAAGAGGTCC ATCGGGACCA
    ATGGGACCAC CGGGAGCTCC CGGAATGCCA GGAATCACCG GAAAACCAGG AAAACCCGGA
    GACACTGGCT TTCCTGGACC TGTTGGCATG AAAGGAGAGA AGGGTGAAAG AGGAGACACT
    GCGTCACAGA ATATGATGCG CTCTGTAGCG AGACAAGTCT GCGAACAGCT AGTCAGCAGA
    CAAATGAGCA GAATTGATAT GATGATGAAC CAGATCCCCA GCAACTACAG GAGTAACAGC
    CCCGGCCCCG CTGGACCTCC AGGCCCACCT GGAAACCAGG GACCCCATGG AGAGCCTGGT
    CAGCCCGGAC CCAATGGCTT CCCAGGAAAT CCAGGTTTAC CAGGCTCTCC TGGAGAAAGA
    GGAGTGCCTG GAGAGAAGGG TGAACGAGGC AGCCCCGGCA TTGGCACTCG AGGACAGAGA
    GGATTACCTG GACCTCCTGG GCCACCAGGG CAGTCTCAGA CCGGTCCCCC TGGTCCCTCA
    GGCTCACCCG GGCCCCGAGG ACCACCAGGA CAACATGGTT CTTCTGGAGT CACAGGACCA
    CCAGGACCTC CAGGATACTG TGACTCCTCT CAGTGCGGCA TGCCTTACAA CGGACAAGGC
    TACCCAGGCC CCTACCAGCC CGGGTCTGAA ACCTACGTAG TGCCCATACC AGCCGCGCCA
    TCGGAAGATA TCGAGACACA GTCGCCAGGA TTGCGCTACC AGCGCGGAAA AAGGTCGCTA
    TCAAGAAAGG AAGCCGAAAG AATCAAAACA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48774
    Clone ID Related Accession (Same CDS sequence) XM_009293298.3 , XM_009293298.2
    Accession Version XM_009293298.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5853bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product collagen alpha-1(XII) chain isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293298.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGAAGATCA GGTTGTCTTT GGCCGCTGCT GCGCTCTTGA CTCTAGTACT CAGTTCAGTA 
    GAGGCTCAAG ATGCCCAGTG TAAAACCACG GCCCAAGCGG ATATCGTCCT GCTTGTTGAT
    GGATCTTGGA GCATTGGTCG CCTGAACTTC AAGACCATAC GCAATTTCAT TGCTCGCATG
    GTGGGAGTCT TCGACATCGG CCCCGATCGT GTCCAGATTG GTCTGGCACA GTACAGCGGA
    GACCCGAAGA CTGAGTGGCA TCTCAATGCT CACAGAACCA GGACTCAGCT GCTGGATGCG
    GTGGCCAATC TACCCTACAA AGGAGGAAAC ACTCTCACCG GCCTTGCTTT GAATTACATC
    CTTCAGAATA ACTTCAAGCC TAACGTTGGC ATGCGTCCAA ACTCCAGAAA GATCGGCGTC
    CTGGTCACTG ATGGCAAGTC CCAAGACGAC ATTGTGGCGA ACTCACAGAA CCTGCGGGAC
    CAAGGGATTG AACTGTACGC CATTGGTGTA AAGAACGCAG ATGAGAATGA GTTGAGAACC
    ATTGCTTCCG ATCCCGATGA CATTCACATG TACAACGTGG CTGATTTCTC CTTCCTGCTG
    GACATTGTGG ATGACCTCAC AAACAACCTG TGCAACAGCG TCAAAGGTCC AGGAGGAGGA
    CCAGAGCCAC CCACTGATCT GACCACCTCA GAGGTCACAC ATTACTCTTT CCGTGCCACT
    TGGGTGGCTC CTGACGGGCC TGTGGAGAAG TACAGAGTGG AGTATGTGAG CACCGCTGGA
    GGAGAACCAC AGCAACAGAT GTTTGTGGAC GGGACGGTGA CCACTGTGGT TTTGGAAGGT
    CTCACTCCGC TGACCGAGTA CATAGTAAAG GTTTACTCAG TGGTGGGAGA GGACAGCAGC
    GACCCACTCA GAGGCATAGA GACCACATTG CCTCTCAGTG CTGTGAGGAG CATGAACATC
    TACAAAGAGC GCTCCACCAC CATGAATGTG AGATGGGACG CAGCCACAGG GGCCACAGGC
    TACATGCTTC TCTACAAGGC CCTTAACGCC ACTGAACCCT CTGTGGAGCA GGAGATGCGA
    GTGAAAGGAA TGGTCACTGA CGTCCAGCTG AAGAAACTTC TCCCCAACAC GGCCTACAGT
    GTTTCGCTCT TCGCCCTGCA CGGCGATTAC GCCAGTGAAC CTCTGATTGA TCAAGGGGTG
    ACCCTGCCTC TTCCTCCATC CGGTGAGTTG AGGATTACCG ATGTGACCCA CAGCACCATG
    CAGCTGGACT GGGACGCTGC GCCCGGCGCC GTCCGAAAGT ACATCATCAC TTACAAGCCA
    GAGGAGGGAG AGGCGAAGGA GCTTGAAGTT GACGGAGATG TAACATCTAG AGGCCTGGAT
    AATCTCATCT CCCAGACTGA ATATGATGTG GCTGTGACTC CGGTTTATGA TTCGGGCACT
    GGAAACCCCA TGCTTAACCA GGCCATTACA GATGTGGTTC CTGCCCCGCA GAACTTGAGG
    TTCTCGATGG TGACCCAGAC CAGTTTTAGG GTTCACTGGG AGCACGGCGC TCCTGATGTC
    TCCCTCTACC GTATTGGCTG GAAAAAGAAA GGAACGACCG AGTTTCAAAA TGATATCCTG
    AGTAGTGAAG ACACTACATA TGATCTGGTG GACCTGGAGC CAGACACACC TTATGATGTT
    TCGGTCACTG CCATTTACCC TGATGAGTCT GAGAGCGAGG ATCTGCTGGG CACTGAACGC
    ACTTTGGCCA AGCTTGCTAC ACCACCTCCC AGTGGTCCTC CTCAAAACCT CCAGGTGTTC
    GACGAGACCA CCACCTCCCT CACGACTAAG TGGGACCACG CGCCTGGACG AGTTCAGAAC
    TACAAAATCA CCTACGTACC GCTTACTGGA GGACGATCAC AGTCGACGCA GGTCGGAGGA
    AAGAAAAACA CAGTGATCCT GCAGAAACTG ACTCCAGACA CTCCTTACTC CATCACTGTT
    GCTGCGGTCT ACGCCAGCGG AGAGGCCAGA GACATTTCTG GCCAAGGAAA GACATTGCCG
    CTGGGCAGTG TCAGGAATCT TCAGGTCACT GACCCCACAG TTAGCACTCT GAATGTGCGC
    TGGGAGCCGG CGGAGGGAAG CGTTCGCCAG TACAGGATCT ACTACCAGCC CGTCGCAGGG
    GGCGCAGAGG ACATGGTCCA GGTTCCTGGA AGCACCACCA GCACCGTTCT GAAGAACCTG
    CAATCTGACA CTCAGTACAG AGTGACGGTG GTGCCAGTCT ACGCTCCTGG AGAGGGCAAG
    CGCATGTCGG AGACCGGAAA AACACTGGTT CGGACCCCTG CCAAGAACAT CCAGGTTTAC
    AACCCCACCA CAAACACCCT GAACGTGCGC TGGGAGCCGG CCTCAGGACA GGTGCAGCAG
    TACCGCGTGG CTTACGCCCC TCTCTCCGGG ATCCGACCCT CTCAGTCGGT GCTGGTTCCA
    GGAAACACCC ACACGGCTCT GCTGCAGCAG CTGGAATCAG ACACTGACTA CTCTGTGAGT
    GTGGTGGCTC TGTATGCTGA TGGAGAGGGC TCGTCTGTGT CTGATAATGG CAAAACACTG
    CCCCGCAGTG GCCCCAGGAA CCTGCGGGTT TATGATCCCA CCACCAGCAC GCTGTCGGTG
    GGCTGGGAAC ATGCAGAAGG GCCTGTGATT CAGTACCGCA TTGGCTACGC ACCCACCACT
    GGAGATCCCA TTGAAGAATT TACAACTGTC CCAGGCAACC GTAACTCGGT CATCCTGCAA
    AACCTGCAGC CAGACACGCC GTACAACATC AACGTAGAAG CCATTTATCA AGATGGCCCT
    GGAGGACGAC TCAGTGGAGA TGGAAAAACA TTGGATCTCC TTGGGCCTCG TAACCTGCGT
    GTTTCAGACG AGTGGTACAC ACGTTTCCGA GTGTCCTGGG ATCCTGCAGC CGCACGGGTG
    AATGGATATA AACTGGTCTA TACACCAGTG GGCACAACCG ACACCATGGA AGCCTTCGTT
    GGAGACGTGA CTTCACACCA CCTGAACAAC CTCCAGCCTG GCACCACATA CGACCTGAAG
    GTTTACGCTC AGTATGATGC GGGCTTGAGC GGCGCTCTGG TCGGACAGGG AACCACCCTG
    TATCTCAACG TGACCGACCT CACTTCCTAC AACGTGGGCT ACGACTCTTT CTGCATCAGA
    TGGACTCCTC ATCGAGCTGC CACCTCCTAC AGACTCAAAG TCAACCCTTT CAACCCATCT
    CACCGTGGAG CTCAGGAGGT CACGATCACT GCGGCTGAGA GCCAGTACTG TTTTGACGGC
    CTGACGCCAG ACGCGCTATA CAACGCCACC GTCTACACGC AGACCCCCAA CCGAGAGGGG
    CCCGGAGTCA GTGTCAAAGA GCGCACACTG GTGAAGCCCA CCGTCGCCCC AACACTACCT
    CCAACTCCTC CACCTCCAGC CACCATTCCT TCTGCACTCG AGGTGTGCAA AGGTGCCAAG
    GCTGACCTGG TGTTCCTGAT CGATGGCTCG TGGAGTATCG GAGATGACAG CTTCGCTAAA
    GTCCGGCAGT TTGTGTTCAG CATGATTGGG GCGTTTGATG TGGTCAGCCA TGAGGGCATG
    CAGGTGTCTT TCGTGCAGTA CAGCGACGAC GCCAAAACTG AGTTCAAGCT CAACACCTAC
    CACGACAAGG GAATGGTGCT GTCTGCTCTT CAGATGGTCA ATTATAGAGG AGGAAACACC
    AAAACAGGTG CTGCACTGAA GCATGTTGGA GAAAAAGTGT TCACCTCGGA CAATGGCATG
    AGGAGAACCG TTCCCAAGGT ACTGGTTGTG GTGACCGATG GACGTTCTCA AGATGACGTG
    AAGAAGAGCG CTGCTAAACT ACAGCATGCA GGTTACAGTG TGTTTGTGGT GGGTGTCGCT
    GACATTGACG TTAATGAACT GAGGAACATT GGAAGCAAGC CCAGTGACAG ACACGTCTTT
    ATCGTCGACA ACTTCGATGC TTTCGCCAAG ATCCAAGACA ACCTGATCAC GTTCATCTGT
    GAAACGGCAA CGTCCACATG CCCTTTGATT TACCTCAATG GATTCACATC ACCAGGCTTC
    AGGATGCTGG AAGCTTTCAA CATCACTGAC AAGACCTACG CAGGCATGAA CGGCGTCTCT
    CTGGAAACCG GCTCCTTCAA CAGCTTCATC GCATACAGAC TCCATAAGGA CGCCTTCATT
    TCCCAGCCAA CAAAGGAAAT CCATCCAGAT GGTCTTCCTC CATCCTACAC CCTCATCCTC
    CTCTTCCGTC TGCTCCCGGA CACCGGCAAG GATGCGTTTG ATTTATGGCA GATCTCAGAT
    AAGAACAATA ACCCTGAGGT CGGGCTCACC ATTGACCCCT CCAGCAGAGT GATCTCCTTC
    TACAATAAAG ACACTAGAGG GGAAATTCAG AAAGTCTCCT TTGATAACGA CCACGTGAAG
    AAGATTTTCC ACGGCAGTTT CCACAAGCTT CACATTTCTA TTTCGCCGAA AAATGTCAAA
    CTCAACATCG ACTGCCAAGA GGTGGCTGAG AAACAGATCA AAGCAGCCAA CAACGTCTCC
    ACAGATGGCT ACGAAGTCCT TGGAAAAATG ACAAAGTCTG GCGGTTCAAA GAAGCAGTCG
    GCCACATTCC AGCTCCAGAT GTTTGATATT GTCTGCAGTT TGGGCTGGAC AAGTCGAGAC
    AAATGTTGTG ATATTCCAGC AATGCGAGAT GAAGCCAAGT GTCCTGCTCT TCCTCACTCC
    TGCACATGTG CTCAGGACAG TGTTGGTCCT CCAGGCCCAG CGGGTCCTCA AGGCAGTCCT
    GGTACAAAAG GCCCACGAGG CGAGAGAGGA GAGTCTGGTC CTACGGGTCC AATGGGTCCA
    CGTGGAGAGC AGGGTCTGCC AGGGCAGATG GGGCCACCGG GTCCACAGGG CCCCAATGGA
    CTATCCCTTC CCGGAGAACC AGGGCGACCG GGACCTAAGG GAGACCCCGG TGACTCAGGT
    TTACCTGGAC AGAGAGGCCC GGTTGGCGCT GCAGGTCCAC TCGGCCCTGT TGGACCCGCT
    GGAGCAAGAG GTCCACCAGG AAGTGAAGGC CCATCTGGAC CAAGAGGTCC ATCGGGACCA
    ATGGGACCAC CGGGAGCTCC CGGAATGCCA GGAATCACCG GAAAACCAGG AAAACCCGGA
    GACACTGGCT TTCCTGGACC TGTTGGCATG AAAGGAGAGA AGGGTGAAAG AGGAGACACT
    GCGTCACAGA ATATGATGCG CTCTGTAGCG AGACAAGTCT GCGAACAGCT AGTCAGCAGA
    CAAATGAGCA GAATTGATAT GATGATGAAC CAGATCCCCA GCAACTACAG GAGTAACAGC
    CCCGGCCCCG CTGGACCTCC AGGCCCACCT GGAAACCAGG GACCCCATGG AGAGCCTGGT
    CAGCCCGGAC CCAATGGCTT CCCAGGAAAT CCAGGTTTAC CAGGCTCTCC TGGAGAAAGA
    GGAGTGCCTG GAGAGAAGGG TGAACGAGGC AGCCCCGGCA TTGGCACTCG AGGACAGAGA
    GGATTACCTG GACCTCCTGG GCCACCAGGG CAGTCTCAGA CCGGTCCCCC TGGTCCCTCA
    GGCTCACCCG GGCCCCGAGG ACCACCAGGA CAACATGGTT CTTCTGGAGT CACAGGACCA
    CCAGGACCTC CAGGATACTG TGACTCCTCT CAGTGCGGCA TGCCTTACAA CGGACAAGGC
    TACCCAGGCC CCTACCAGCC CGGGTCTGAA ACCTACGTAG TGCCCATACC AGCCGCGCCA
    TCGGAAGATA TCGAGACACA GTCGCCAGGA TTGCGCTACC AGCGCGGAAA AAGGTCGCTA
    TCAAGAAAGG AAGCCGAAAG AATCAAAACA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009291573.1
    CDS137..5989
    Translation

    Target ORF information:

    RefSeq Version XM_009293298.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio collagen, type XII, alpha 1a (col12a1a), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009293298.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGAAGATCA GGTTGTCTTT GGCCGCTGCT GCGCTCTTGA CTCTAGTACT CAGTTCAGTA 
    GAGGCTCAAG ATGCCCAGTG TAAAACCACG GCCCAAGCGG ATATCGTCCT GCTTGTTGAT
    GGATCTTGGA GCATTGGTCG CCTGAACTTC AAGACCATAC GCAATTTCAT TGCTCGCATG
    GTGGGAGTCT TCGACATCGG CCCCGATCGT GTCCAGATTG GTCTGGCACA GTACAGCGGA
    GACCCGAAGA CTGAGTGGCA TCTCAATGCT CACAGAACCA GGACTCAGCT GCTGGATGCG
    GTGGCCAATC TACCCTACAA AGGAGGAAAC ACTCTCACCG GCCTTGCTTT GAATTACATC
    CTTCAGAATA ACTTCAAGCC TAACGTTGGC ATGCGTCCAA ACTCCAGAAA GATCGGCGTC
    CTGGTCACTG ATGGCAAGTC CCAAGACGAC ATTGTGGCGA ACTCACAGAA CCTGCGGGAC
    CAAGGGATTG AACTGTACGC CATTGGTGTA AAGAACGCAG ATGAGAATGA GTTGAGAACC
    ATTGCTTCCG ATCCCGATGA CATTCACATG TACAACGTGG CTGATTTCTC CTTCCTGCTG
    GACATTGTGG ATGACCTCAC AAACAACCTG TGCAACAGCG TCAAAGGTCC AGGAGGAGGA
    CCAGAGCCAC CCACTGATCT GACCACCTCA GAGGTCACAC ATTACTCTTT CCGTGCCACT
    TGGGTGGCTC CTGACGGGCC TGTGGAGAAG TACAGAGTGG AGTATGTGAG CACCGCTGGA
    GGAGAACCAC AGCAACAGAT GTTTGTGGAC GGGACGGTGA CCACTGTGGT TTTGGAAGGT
    CTCACTCCGC TGACCGAGTA CATAGTAAAG GTTTACTCAG TGGTGGGAGA GGACAGCAGC
    GACCCACTCA GAGGCATAGA GACCACATTG CCTCTCAGTG CTGTGAGGAG CATGAACATC
    TACAAAGAGC GCTCCACCAC CATGAATGTG AGATGGGACG CAGCCACAGG GGCCACAGGC
    TACATGCTTC TCTACAAGGC CCTTAACGCC ACTGAACCCT CTGTGGAGCA GGAGATGCGA
    GTGAAAGGAA TGGTCACTGA CGTCCAGCTG AAGAAACTTC TCCCCAACAC GGCCTACAGT
    GTTTCGCTCT TCGCCCTGCA CGGCGATTAC GCCAGTGAAC CTCTGATTGA TCAAGGGGTG
    ACCCTGCCTC TTCCTCCATC CGGTGAGTTG AGGATTACCG ATGTGACCCA CAGCACCATG
    CAGCTGGACT GGGACGCTGC GCCCGGCGCC GTCCGAAAGT ACATCATCAC TTACAAGCCA
    GAGGAGGGAG AGGCGAAGGA GCTTGAAGTT GACGGAGATG TAACATCTAG AGGCCTGGAT
    AATCTCATCT CCCAGACTGA ATATGATGTG GCTGTGACTC CGGTTTATGA TTCGGGCACT
    GGAAACCCCA TGCTTAACCA GGCCATTACA GATGTGGTTC CTGCCCCGCA GAACTTGAGG
    TTCTCGATGG TGACCCAGAC CAGTTTTAGG GTTCACTGGG AGCACGGCGC TCCTGATGTC
    TCCCTCTACC GTATTGGCTG GAAAAAGAAA GGAACGACCG AGTTTCAAAA TGATATCCTG
    AGTAGTGAAG ACACTACATA TGATCTGGTG GACCTGGAGC CAGACACACC TTATGATGTT
    TCGGTCACTG CCATTTACCC TGATGAGTCT GAGAGCGAGG ATCTGCTGGG CACTGAACGC
    ACTTTGGCCA AGCTTGCTAC ACCACCTCCC AGTGGTCCTC CTCAAAACCT CCAGGTGTTC
    GACGAGACCA CCACCTCCCT CACGACTAAG TGGGACCACG CGCCTGGACG AGTTCAGAAC
    TACAAAATCA CCTACGTACC GCTTACTGGA GGACGATCAC AGTCGACGCA GGTCGGAGGA
    AAGAAAAACA CAGTGATCCT GCAGAAACTG ACTCCAGACA CTCCTTACTC CATCACTGTT
    GCTGCGGTCT ACGCCAGCGG AGAGGCCAGA GACATTTCTG GCCAAGGAAA GACATTGCCG
    CTGGGCAGTG TCAGGAATCT TCAGGTCACT GACCCCACAG TTAGCACTCT GAATGTGCGC
    TGGGAGCCGG CGGAGGGAAG CGTTCGCCAG TACAGGATCT ACTACCAGCC CGTCGCAGGG
    GGCGCAGAGG ACATGGTCCA GGTTCCTGGA AGCACCACCA GCACCGTTCT GAAGAACCTG
    CAATCTGACA CTCAGTACAG AGTGACGGTG GTGCCAGTCT ACGCTCCTGG AGAGGGCAAG
    CGCATGTCGG AGACCGGAAA AACACTGGTT CGGACCCCTG CCAAGAACAT CCAGGTTTAC
    AACCCCACCA CAAACACCCT GAACGTGCGC TGGGAGCCGG CCTCAGGACA GGTGCAGCAG
    TACCGCGTGG CTTACGCCCC TCTCTCCGGG ATCCGACCCT CTCAGTCGGT GCTGGTTCCA
    GGAAACACCC ACACGGCTCT GCTGCAGCAG CTGGAATCAG ACACTGACTA CTCTGTGAGT
    GTGGTGGCTC TGTATGCTGA TGGAGAGGGC TCGTCTGTGT CTGATAATGG CAAAACACTG
    CCCCGCAGTG GCCCCAGGAA CCTGCGGGTT TATGATCCCA CCACCAGCAC GCTGTCGGTG
    GGCTGGGAAC ATGCAGAAGG GCCTGTGATT CAGTACCGCA TTGGCTACGC ACCCACCACT
    GGAGATCCCA TTGAAGAATT TACAACTGTC CCAGGCAACC GTAACTCGGT CATCCTGCAA
    AACCTGCAGC CAGACACGCC GTACAACATC AACGTAGAAG CCATTTATCA AGATGGCCCT
    GGAGGACGAC TCAGTGGAGA TGGAAAAACA TTGGATCTCC TTGGGCCTCG TAACCTGCGT
    GTTTCAGACG AGTGGTACAC ACGTTTCCGA GTGTCCTGGG ATCCTGCAGC CGCACGGGTG
    AATGGATATA AACTGGTCTA TACACCAGTG GGCACAACCG ACACCATGGA AGCCTTCGTT
    GGAGACGTGA CTTCACACCA CCTGAACAAC CTCCAGCCTG GCACCACATA CGACCTGAAG
    GTTTACGCTC AGTATGATGC GGGCTTGAGC GGCGCTCTGG TCGGACAGGG AACCACCCTG
    TATCTCAACG TGACCGACCT CACTTCCTAC AACGTGGGCT ACGACTCTTT CTGCATCAGA
    TGGACTCCTC ATCGAGCTGC CACCTCCTAC AGACTCAAAG TCAACCCTTT CAACCCATCT
    CACCGTGGAG CTCAGGAGGT CACGATCACT GCGGCTGAGA GCCAGTACTG TTTTGACGGC
    CTGACGCCAG ACGCGCTATA CAACGCCACC GTCTACACGC AGACCCCCAA CCGAGAGGGG
    CCCGGAGTCA GTGTCAAAGA GCGCACACTG GTGAAGCCCA CCGTCGCCCC AACACTACCT
    CCAACTCCTC CACCTCCAGC CACCATTCCT TCTGCACTCG AGGTGTGCAA AGGTGCCAAG
    GCTGACCTGG TGTTCCTGAT CGATGGCTCG TGGAGTATCG GAGATGACAG CTTCGCTAAA
    GTCCGGCAGT TTGTGTTCAG CATGATTGGG GCGTTTGATG TGGTCAGCCA TGAGGGCATG
    CAGGTGTCTT TCGTGCAGTA CAGCGACGAC GCCAAAACTG AGTTCAAGCT CAACACCTAC
    CACGACAAGG GAATGGTGCT GTCTGCTCTT CAGATGGTCA ATTATAGAGG AGGAAACACC
    AAAACAGGTG CTGCACTGAA GCATGTTGGA GAAAAAGTGT TCACCTCGGA CAATGGCATG
    AGGAGAACCG TTCCCAAGGT ACTGGTTGTG GTGACCGATG GACGTTCTCA AGATGACGTG
    AAGAAGAGCG CTGCTAAACT ACAGCATGCA GGTTACAGTG TGTTTGTGGT GGGTGTCGCT
    GACATTGACG TTAATGAACT GAGGAACATT GGAAGCAAGC CCAGTGACAG ACACGTCTTT
    ATCGTCGACA ACTTCGATGC TTTCGCCAAG ATCCAAGACA ACCTGATCAC GTTCATCTGT
    GAAACGGCAA CGTCCACATG CCCTTTGATT TACCTCAATG GATTCACATC ACCAGGCTTC
    AGGATGCTGG AAGCTTTCAA CATCACTGAC AAGACCTACG CAGGCATGAA CGGCGTCTCT
    CTGGAAACCG GCTCCTTCAA CAGCTTCATC GCATACAGAC TCCATAAGGA CGCCTTCATT
    TCCCAGCCAA CAAAGGAAAT CCATCCAGAT GGTCTTCCTC CATCCTACAC CCTCATCCTC
    CTCTTCCGTC TGCTCCCGGA CACCGGCAAG GATGCGTTTG ATTTATGGCA GATCTCAGAT
    AAGAACAATA ACCCTGAGGT CGGGCTCACC ATTGACCCCT CCAGCAGAGT GATCTCCTTC
    TACAATAAAG ACACTAGAGG GGAAATTCAG AAAGTCTCCT TTGATAACGA CCACGTGAAG
    AAGATTTTCC ACGGCAGTTT CCACAAGCTT CACATTTCTA TTTCGCCGAA AAATGTCAAA
    CTCAACATCG ACTGCCAAGA GGTGGCTGAG AAACAGATCA AAGCAGCCAA CAACGTCTCC
    ACAGATGGCT ACGAAGTCCT TGGAAAAATG ACAAAGTCTG GCGGTTCAAA GAAGCAGTCG
    GCCACATTCC AGCTCCAGAT GTTTGATATT GTCTGCAGTT TGGGCTGGAC AAGTCGAGAC
    AAATGTTGTG ATATTCCAGC AATGCGAGAT GAAGCCAAGT GTCCTGCTCT TCCTCACTCC
    TGCACATGTG CTCAGGACAG TGTTGGTCCT CCAGGCCCAG CGGGTCCTCA AGGCAGTCCT
    GGTACAAAAG GCCCACGAGG CGAGAGAGGA GAGTCTGGTC CTACGGGTCC AATGGGTCCA
    CGTGGAGAGC AGGGTCTGCC AGGGCAGATG GGGCCACCGG GTCCACAGGG CCCCAATGGA
    CTATCCCTTC CCGGAGAACC AGGGCGACCG GGACCTAAGG GAGACCCCGG TGACTCAGGT
    TTACCTGGAC AGAGAGGCCC GGTTGGCGCT GCAGGTCCAC TCGGCCCTGT TGGACCCGCT
    GGAGCAAGAG GTCCACCAGG AAGTGAAGGC CCATCTGGAC CAAGAGGTCC ATCGGGACCA
    ATGGGACCAC CGGGAGCTCC CGGAATGCCA GGAATCACCG GAAAACCAGG AAAACCCGGA
    GACACTGGCT TTCCTGGACC TGTTGGCATG AAAGGAGAGA AGGGTGAAAG AGGAGACACT
    GCGTCACAGA ATATGATGCG CTCTGTAGCG AGACAAGTCT GCGAACAGCT AGTCAGCAGA
    CAAATGAGCA GAATTGATAT GATGATGAAC CAGATCCCCA GCAACTACAG GAGTAACAGC
    CCCGGCCCCG CTGGACCTCC AGGCCCACCT GGAAACCAGG GACCCCATGG AGAGCCTGGT
    CAGCCCGGAC CCAATGGCTT CCCAGGAAAT CCAGGTTTAC CAGGCTCTCC TGGAGAAAGA
    GGAGTGCCTG GAGAGAAGGG TGAACGAGGC AGCCCCGGCA TTGGCACTCG AGGACAGAGA
    GGATTACCTG GACCTCCTGG GCCACCAGGG CAGTCTCAGA CCGGTCCCCC TGGTCCCTCA
    GGCTCACCCG GGCCCCGAGG ACCACCAGGA CAACATGGTT CTTCTGGAGT CACAGGACCA
    CCAGGACCTC CAGGATACTG TGACTCCTCT CAGTGCGGCA TGCCTTACAA CGGACAAGGC
    TACCCAGGCC CCTACCAGCC CGGGTCTGAA ACCTACGTAG TGCCCATACC AGCCGCGCCA
    TCGGAAGATA TCGAGACACA GTCGCCAGGA TTGCGCTACC AGCGCGGAAA AAGGTCGCTA
    TCAAGAAAGG AAGCCGAAAG AATCAAAACA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa22912
    Clone ID Related Accession (Same CDS sequence) XM_002665259.6 , XM_002665259.5
    Accession Version XM_002665259.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9204bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product collagen alpha-1(XII) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_002665259.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGAAGATCA GGTTGTCTTT GGCCGCTGCT GCGCTCTTGA CTCTAGTACT CAGTTCAGTA 
    GAGGCTCAAG TGGAGCCTCC GTCAGACTTA AAATTTAAAA TCCTGAATGA AAACACAGTG
    CAAATGTCAT GGAGGAAGCC CTCGTCTCAG ATACAGGGCT ACAGAATACA GGTCACGTCA
    GATACAGATG ATTCTAAAGA ATTGAGTCTT CCTGCCTCGT CCACAAGCAC ATCAATTACA
    GATCTAACTC CAGATGTAGA CTATTCAGTG TCAATCAATT CATATGATGG TGCTGAAGAA
    AGTATCCCTA TCCTTGGACA AATCACAATT CAATCCAGCA ACACAAGTGG ACCAAGGAGG
    CCACAGTCAG AGGCTGTCAA GTGTTCAGTG AGTGCCGTTG CGGATCTGGT TTTCCTGGTG
    GACGGCTCTT GGAGTGTGGG AAGAGAAAAC TTTAGATTTA TCCGGAGTTT CATTGGGGCC
    ATGGCCGGGG CCTTCGACAT TGAAGAGGAT AAGACCAGAG TGGCTGTGGT TCAGTACAGC
    TCGGACACCA GGACAGAGTT CAGTCTAAAC ACACATTTCA GACGACCCGA TGTCCTGCGA
    GCCATTAAAA ATCTGCCGTA CAAGGGTGGA AATACCATGA CGGGTGATGC AATGGACTAT
    CTGGTGAAAA ACACGTTCAC TCAGGCGGCT GGAGCCAGGA AAGGCTTCCC AAAAGTGGCC
    ATGATCATCA CAGATGGAAA ATCTCAAGAC CCCGTAGAGG AGTACGCCGA ACGGTTGAGG
    AACATCGGAG TGGAGATCTT TGTGCTGGGT ATCAAAGGAG CAGATGAGGA CGAGCTGAAG
    GAGATCGCCT CCAGACCCCA TAGCAAGCAT GTCTATAACG TCCCCAACTT CGACATGATC
    AATCAGGTCC AGAAGGCGCT CATCACTGAA GTTTGCTCTG GCGTGGAGGA GCAGCTAAAT
    TCCCTTGTAA GCGGGGAGGA AGTTGTCGAA CCAGCTTCCA ACCTCAGAGT GACAGATATC
    TCCTCCAAAT CCATGAGAAT TGCGTGGGAT TCATCTCCTG GTGATATCAC AGGCTATAAG
    CTGCAGGTTT TTCCCACTAT TGCGAGCGCC AAAAAGCAGG AGCTCTACAC TGGGCCCACG
    ATGACCTTGG CCAATGTCAG AGACCTGACG CCTGAGATGG AGTATGAGAT CAGTCTGTTT
    GCTCTCAAAG GTCTGACTCC TAGTGTGCCG CAAGTTGTCA CTGAGAAAAC TCAGCCAGTG
    AAAGTATTTA CAGAGTGTTC CCTAGAGGTG GATGTCCAAG CTGACATTGT CCTCCTGGTG
    GATGGGTCAT ATAGTATAGG ACTGCCTAAC TTTGCGAAGG TTAGAGCTTT CCTCGAAGTA
    CTTGTGAATT CTTTTGACAT CGGACCTAAC AAAGTGCAGA TCAGCTTGGT TCAGTACAGC
    AGGGATCCTC ACACTGAGTT CGCCTTGAAT AAATTCGATG ACAATGCAGC CATGGTCAAG
    GCGGTCCGCA CCTTCCCCTA CCGAGGTGGA TCCACCAACA CCGGCAAGGC CATGACATAT
    GTGAGGGAAA AGATCTTTGT TTCGGGCCGT GGCGCTCGTG ATAACGTCCC CCGAGTCATG
    GTGCTTATCA CAGACGGCAA GTCCTCAGAT TCTTTCAAAG ACCCCGCCAA CAAACTGAGG
    GATACAGATG TGGAGATTTT TGCAGTGGGA GTAAAAGATG CCGTACGATC TGAGCTGGAG
    GCTATCGCCA ATGTTCCTGC TGATAACCAT GTGTTTGAGG TGGAGGACTT TGATGCTTTC
    GAGCGGATCT CCAAGGAGCT GACCGCGTCC ATCTGCCTCA GAATTGAGCA GGAGCTCCAT
    AACATCAGGA AAAGAAGTTT GCTTCCCCCA AAGTCACTGA GCTTCTCTGA GGTGACCTCC
    AGGAGTTTCA GGACCTCATG GGTTTCTGAT GGAGAGGATA TCCTGTCTTT TCTGGTGCGC
    TTCAGGCCAG CGGCGGACCT AACTGGAGAC TACATCTCTC TGGTGGTTCC CCATGACATC
    ACCAGTACAA TTCTGCCCAA TCTCACGCCA CTCACCACCT ACGAAGTTAA CGTCATTGCT
    CAGTATGATA AAGGAGACAG CTTTCCTTTG ACGGGCGAGG AGACCACTTT AGAAGAGTTG
    GGTTCTGTTC GTAACCTGAT GGTGAGCGAG GAGGCTGTGG ACCGCTTTCG GGTGTCGTGG
    AGAGCTGCTC CAGGATTAGT GCTGAGGTAC AGACTGCTCT ACGAGCCTGT GAGCGGAGGG
    GATAAACTGG AAGCAGAGAC TGATGGGAAT CAGGTCACCA CTGTGCTCAA TGAACTACTA
    CCGGTCACTA CTTACCGGGT CACCGTTTAC CCTGAGTACG CAAGTGGAAG GGGTCCCTCC
    ATGGACACTG AAGGCACCAC CAAAGAAGTT CGAGGTTCTC CACGCGACTT AAGGGTCTTC
    AATGAGACGG TGTCTAGCAT GAGGGTCTCG TGGCGGGCTG CCCCAGGCAA CGTGCTTCAA
    TATCAGGTGG CCTACAAACC TGACGGTGGG GAGAGGAAAG AAATAAGTGT TAAAGGAGAG
    ACAACAACTG CTGTGCTGAA AAATCTGCTG CCGGACACGG AGTATGAGCT GTTTGTTAGC
    GCACGTTACG TCTCTGGACT TGGCGCACCA CTTATGGGAA CAGGCACCAC GCTGGAAGAA
    CTTGGTTCAC CTTCGAATCT GGTAACTTCA GACGTCACTG AGAACAGTTT TGTAGCATCC
    TGGACCGCCG CCCCTGGAAA CGTCCGTCTT TATCGGGTCA CCTGGAAATC CCTCTTTTCA
    GAGGAAGCTG GCGAGAAGAC TGTCCCCGGG GACGTAACCA CCACTGTTCT GGACGGGCTG
    ACACCAGAGA CCCGCTACAA GGTTTCTGTC TACGCTGTCT ACAGCCGTGC GGAGGGGGCG
    CCACTGCACG GAGAGGAGAC CACAGATGTG TCGGCCGCTA CCAGAGCTCT TACGGTGTCA
    GAAGAAACAG AGAGGACCAT GAAGGTGACA TGGATACCAG CACCTGGCAA TGTTGTCAAT
    TACAGGGTAT CATACAAACC TCAGGGTGAT TTACGCCAAA TGGCCTCCAA AGCACCAGGA
    GGCACCAATT CTATAGTCCT GAGACGCCTG ATCCCCCAGA CCACTTACGA TATCACAGTG
    GTTCCCGTCT ACAAGTTTGG GGATGGAAGA CAAAGGCAAG GAGAAGGAAC TACATTGTCT
    CAATATAAAG CTCCCAGAAA CTTGCAAACT TCGGAGCCCA CTAAAACCAG TTTCAGGGTG
    ACCTGGGATC ATGCTCCTGG AGATGTAAAA GGCTACAAGG TCACTTTCCA TCCCACCGGA
    AAGGAAGTGG ACCTGGGAGA GTTACTCGTA GGCCCCTATG ACAACACTGT GGTTCTGGAG
    GAACTAAGGG CCGGCACTAA GTATACGGTA AATGTGTTTG GTATGTTTGA TGGAGGAGAG
    AGTATGCCAT TGGCTGGAGA GGAGAAGACC ACCCTCTCTG ACGCAGCTTC TGAAAACCTC
    CCTTATACAC CTTCAGATGC CCAGTGTAAA ACCACGGCCC AAGCGGATAT CGTCCTGCTT
    GTTGATGGAT CTTGGAGCAT TGGTCGCCTG AACTTCAAGA CCATACGCAA TTTCATTGCT
    CGCATGGTGG GAGTCTTCGA CATCGGCCCC GATCGTGTCC AGATTGGTCT GGCACAGTAC
    AGCGGAGACC CGAAGACTGA GTGGCATCTC AATGCTCACA GAACCAGGAC TCAGCTGCTG
    GATGCGGTGG CCAATCTACC CTACAAAGGA GGAAACACTC TCACCGGCCT TGCTTTGAAT
    TACATCCTTC AGAATAACTT CAAGCCTAAC GTTGGCATGC GTCCAAACTC CAGAAAGATC
    GGCGTCCTGG TCACTGATGG CAAGTCCCAA GACGACATTG TGGCGAACTC ACAGAACCTG
    CGGGACCAAG GGATTGAACT GTACGCCATT GGTGTAAAGA ACGCAGATGA GAATGAGTTG
    AGAACCATTG CTTCCGATCC CGATGACATT CACATGTACA ACGTGGCTGA TTTCTCCTTC
    CTGCTGGACA TTGTGGATGA CCTCACAAAC AACCTGTGCA ACAGCGTCAA AGGTCCAGGA
    GGAGGACCAG AGCCACCCAC TGATCTGACC ACCTCAGAGG TCACACATTA CTCTTTCCGT
    GCCACTTGGG TGGCTCCTGA CGGGCCTGTG GAGAAGTACA GAGTGGAGTA TGTGAGCACC
    GCTGGAGGAG AACCACAGCA ACAGATGTTT GTGGACGGGA CGGTGACCAC TGTGGTTTTG
    GAAGGTCTCA CTCCGCTGAC CGAGTACATA GTAAAGGTTT ACTCAGTGGT GGGAGAGGAC
    AGCAGCGACC CACTCAGAGG CATAGAGACC ACATTGCCTC TCAGTGCTGT GAGGAGCATG
    AACATCTACA AAGAGCGCTC CACCACCATG AATGTGAGAT GGGACGCAGC CACAGGGGCC
    ACAGGCTACA TGCTTCTCTA CAAGGCCCTT AACGCCACTG AACCCTCTGT GGAGCAGGAG
    ATGCGAGTGA AAGGAATGGT CACTGACGTC CAGCTGAAGA AACTTCTCCC CAACACGGCC
    TACAGTGTTT CGCTCTTCGC CCTGCACGGC GATTACGCCA GTGAACCTCT GATTGATCAA
    GGGGTGACCC TGCCTCTTCC TCCATCCGGT GAGTTGAGGA TTACCGATGT GACCCACAGC
    ACCATGCAGC TGGACTGGGA CGCTGCGCCC GGCGCCGTCC GAAAGTACAT CATCACTTAC
    AAGCCAGAGG AGGGAGAGGC GAAGGAGCTT GAAGTTGACG GAGATGTAAC ATCTAGAGGC
    CTGGATAATC TCATCTCCCA GACTGAATAT GATGTGGCTG TGACTCCGGT TTATGATTCG
    GGCACTGGAA ACCCCATGCT TAACCAGGCC ATTACAGATG TGGTTCCTGC CCCGCAGAAC
    TTGAGGTTCT CGATGGTGAC CCAGACCAGT TTTAGGGTTC ACTGGGAGCA CGGCGCTCCT
    GATGTCTCCC TCTACCGTAT TGGCTGGAAA AAGAAAGGAA CGACCGAGTT TCAAAATGAT
    ATCCTGAGTA GTGAAGACAC TACATATGAT CTGGTGGACC TGGAGCCAGA CACACCTTAT
    GATGTTTCGG TCACTGCCAT TTACCCTGAT GAGTCTGAGA GCGAGGATCT GCTGGGCACT
    GAACGCACTT TGGCCAAGCT TGCTACACCA CCTCCCAGTG GTCCTCCTCA AAACCTCCAG
    GTGTTCGACG AGACCACCAC CTCCCTCACG ACTAAGTGGG ACCACGCGCC TGGACGAGTT
    CAGAACTACA AAATCACCTA CGTACCGCTT ACTGGAGGAC GATCACAGTC GACGCAGGTC
    GGAGGAAAGA AAAACACAGT GATCCTGCAG AAACTGACTC CAGACACTCC TTACTCCATC
    ACTGTTGCTG CGGTCTACGC CAGCGGAGAG GCCAGAGACA TTTCTGGCCA AGGAAAGACA
    TTGCCGCTGG GCAGTGTCAG GAATCTTCAG GTCACTGACC CCACAGTTAG CACTCTGAAT
    GTGCGCTGGG AGCCGGCGGA GGGAAGCGTT CGCCAGTACA GGATCTACTA CCAGCCCGTC
    GCAGGGGGCG CAGAGGACAT GGTCCAGGTT CCTGGAAGCA CCACCAGCAC CGTTCTGAAG
    AACCTGCAAT CTGACACTCA GTACAGAGTG ACGGTGGTGC CAGTCTACGC TCCTGGAGAG
    GGCAAGCGCA TGTCGGAGAC CGGAAAAACA CTGGTTCGGA CCCCTGCCAA GAACATCCAG
    GTTTACAACC CCACCACAAA CACCCTGAAC GTGCGCTGGG AGCCGGCCTC AGGACAGGTG
    CAGCAGTACC GCGTGGCTTA CGCCCCTCTC TCCGGGATCC GACCCTCTCA GTCGGTGCTG
    GTTCCAGGAA ACACCCACAC GGCTCTGCTG CAGCAGCTGG AATCAGACAC TGACTACTCT
    GTGAGTGTGG TGGCTCTGTA TGCTGATGGA GAGGGCTCGT CTGTGTCTGA TAATGGCAAA
    ACACTGCCCC GCAGTGGCCC CAGGAACCTG CGGGTTTATG ATCCCACCAC CAGCACGCTG
    TCGGTGGGCT GGGAACATGC AGAAGGGCCT GTGATTCAGT ACCGCATTGG CTACGCACCC
    ACCACTGGAG ATCCCATTGA AGAATTTACA ACTGTCCCAG GCAACCGTAA CTCGGTCATC
    CTGCAAAACC TGCAGCCAGA CACGCCGTAC AACATCAACG TAGAAGCCAT TTATCAAGAT
    GGCCCTGGAG GACGACTCAG TGGAGATGGA AAAACATTGG ATCTCCTTGG GCCTCGTAAC
    CTGCGTGTTT CAGACGAGTG GTACACACGT TTCCGAGTGT CCTGGGATCC TGCAGCCGCA
    CGGGTGAATG GATATAAACT GGTCTATACA CCAGTGGGCA CAACCGACAC CATGGAAGCC
    TTCGTTGGAG ACGTGACTTC ACACCACCTG AACAACCTCC AGCCTGGCAC CACATACGAC
    CTGAAGGTTT ACGCTCAGTA TGATGCGGGC TTGAGCGGCG CTCTGGTCGG ACAGGGAACC
    ACCCTGTATC TCAACGTGAC CGACCTCACT TCCTACAACG TGGGCTACGA CTCTTTCTGC
    ATCAGATGGA CTCCTCATCG AGCTGCCACC TCCTACAGAC TCAAAGTCAA CCCTTTCAAC
    CCATCTCACC GTGGAGCTCA GGAGGTCACG ATCACTGCGG CTGAGAGCCA GTACTGTTTT
    GACGGCCTGA CGCCAGACGC GCTATACAAC GCCACCGTCT ACACGCAGAC CCCCAACCGA
    GAGGGGCCCG GAGTCAGTGT CAAAGAGCGC ACACTGGTGA AGCCCACCGT CGCCCCAACA
    CTACCTCCAA CTCCTCCACC TCCAGCCACC ATTCCTTCTG CACTCGAGGT GTGCAAAGGT
    GCCAAGGCTG ACCTGGTGTT CCTGATCGAT GGCTCGTGGA GTATCGGAGA TGACAGCTTC
    GCTAAAGTCC GGCAGTTTGT GTTCAGCATG ATTGGGGCGT TTGATGTGGT CAGCCATGAG
    GGCATGCAGG TGTCTTTCGT GCAGTACAGC GACGACGCCA AAACTGAGTT CAAGCTCAAC
    ACCTACCACG ACAAGGGAAT GGTGCTGTCT GCTCTTCAGA TGGTCAATTA TAGAGGAGGA
    AACACCAAAA CAGGTGCTGC ACTGAAGCAT GTTGGAGAAA AAGTGTTCAC CTCGGACAAT
    GGCATGAGGA GAACCGTTCC CAAGGTACTG GTTGTGGTGA CCGATGGACG TTCTCAAGAT
    GACGTGAAGA AGAGCGCTGC TAAACTACAG CATGCAGGTT ACAGTGTGTT TGTGGTGGGT
    GTCGCTGACA TTGACGTTAA TGAACTGAGG AACATTGGAA GCAAGCCCAG TGACAGACAC
    GTCTTTATCG TCGACAACTT CGATGCTTTC GCCAAGATCC AAGACAACCT GATCACGTTC
    ATCTGTGAAA CGGCAACGTC CACATGCCCT TTGATTTACC TCAATGGATT CACATCACCA
    GGCTTCAGGA TGCTGGAAGC TTTCAACATC ACTGACAAGA CCTACGCAGG CATGAACGGC
    GTCTCTCTGG AAACCGGCTC CTTCAACAGC TTCATCGCAT ACAGACTCCA TAAGGACGCC
    TTCATTTCCC AGCCAACAAA GGAAATCCAT CCAGATGGTC TTCCTCCATC CTACACCCTC
    ATCCTCCTCT TCCGTCTGCT CCCGGACACC GGCAAGGATG CGTTTGATTT ATGGCAGATC
    TCAGATAAGA ACAATAACCC TGAGGTCGGG CTCACCATTG ACCCCTCCAG CAGAGTGATC
    TCCTTCTACA ATAAAGACAC TAGAGGGGAA ATTCAGAAAG TCTCCTTTGA TAACGACCAC
    GTGAAGAAGA TTTTCCACGG CAGTTTCCAC AAGCTTCACA TTTCTATTTC GCCGAAAAAT
    GTCAAACTCA ACATCGACTG CCAAGAGGTG GCTGAGAAAC AGATCAAAGC AGCCAACAAC
    GTCTCCACAG ATGGCTACGA AGTCCTTGGA AAAATGACAA AGTCTGGCGG TTCAAAGAAG
    CAGTCGGCCA CATTCCAGCT CCAGATGTTT GATATTGTCT GCAGTTTGGG CTGGACAAGT
    CGAGACAAAT GTTGTGATAT TCCAGCAATG CGAGATGAAG CCAAGTGTCC TGCTCTTCCT
    CACTCCTGCA CATGTGCTCA GGACAGTGTT GGTCCTCCAG GCCCAGCGGG TCCTCAAGGC
    AGTCCTGGTA CAAAAGGCCC ACGAGGCGAG AGAGGAGAGT CTGGTCCTAC GGGTCCAATG
    GGTCCACGTG GAGAGCAGGG TCTGCCAGGG CAGATGGGGC CACCGGGTCC ACAGGGCCCC
    AATGGACTAT CCCTTCCCGG AGAACCAGGG CGACCGGGAC CTAAGGGAGA CCCCGGTGAC
    TCAGGTTTAC CTGGACAGAG AGGCCCGGTT GGCGCTGCAG GTCCACTCGG CCCTGTTGGA
    CCCGCTGGAG CAAGAGGTCC ACCAGGAAGT GAAGGCCCAT CTGGACCAAG AGGTCCATCG
    GGACCAATGG GACCACCGGG AGCTCCCGGA ATGCCAGGAA TCACCGGAAA ACCAGGAAAA
    CCCGGAGACA CTGGCTTTCC TGGACCTGTT GGCATGAAAG GAGAGAAGGG TGAAAGAGGA
    GACACTGCGT CACAGAATAT GATGCGCTCT GTAGCGAGAC AAGTCTGCGA ACAGCTAGTC
    AGCAGACAAA TGAGCAGAAT TGATATGATG ATGAACCAGA TCCCCAGCAA CTACAGGAGT
    AACAGCCCCG GCCCCGCTGG ACCTCCAGGC CCACCTGGAA ACCAGGGACC CCATGGAGAG
    CCTGGTCAGC CCGGACCCAA TGGCTTCCCA GGAAATCCAG GTTTACCAGG CTCTCCTGGA
    GAAAGAGGAG TGCCTGGAGA GAAGGGTGAA CGAGGCAGCC CCGGCATTGG CACTCGAGGA
    CAGAGAGGAT TACCTGGACC TCCTGGGCCA CCAGGGCAGT CTCAGACCGG TCCCCCTGGT
    CCCTCAGGCT CACCCGGGCC CCGAGGACCA CCAGGACAAC ATGGTTCTTC TGGAGTCACA
    GGACCACCAG GACCTCCAGG ATACTGTGAC TCCTCTCAGT GCGGCATGCC TTACAACGGA
    CAAGGCTACC CAGGTTTTGC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002665305.3
    CDS180..9383
    Translation

    Target ORF information:

    RefSeq Version XM_002665259.6
    Organism Danio rerio(zebrafish)
    Definition Danio rerio collagen, type XII, alpha 1a (col12a1a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002665259.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGAAGATCA GGTTGTCTTT GGCCGCTGCT GCGCTCTTGA CTCTAGTACT CAGTTCAGTA 
    GAGGCTCAAG TGGAGCCTCC GTCAGACTTA AAATTTAAAA TCCTGAATGA AAACACAGTG
    CAAATGTCAT GGAGGAAGCC CTCGTCTCAG ATACAGGGCT ACAGAATACA GGTCACGTCA
    GATACAGATG ATTCTAAAGA ATTGAGTCTT CCTGCCTCGT CCACAAGCAC ATCAATTACA
    GATCTAACTC CAGATGTAGA CTATTCAGTG TCAATCAATT CATATGATGG TGCTGAAGAA
    AGTATCCCTA TCCTTGGACA AATCACAATT CAATCCAGCA ACACAAGTGG ACCAAGGAGG
    CCACAGTCAG AGGCTGTCAA GTGTTCAGTG AGTGCCGTTG CGGATCTGGT TTTCCTGGTG
    GACGGCTCTT GGAGTGTGGG AAGAGAAAAC TTTAGATTTA TCCGGAGTTT CATTGGGGCC
    ATGGCCGGGG CCTTCGACAT TGAAGAGGAT AAGACCAGAG TGGCTGTGGT TCAGTACAGC
    TCGGACACCA GGACAGAGTT CAGTCTAAAC ACACATTTCA GACGACCCGA TGTCCTGCGA
    GCCATTAAAA ATCTGCCGTA CAAGGGTGGA AATACCATGA CGGGTGATGC AATGGACTAT
    CTGGTGAAAA ACACGTTCAC TCAGGCGGCT GGAGCCAGGA AAGGCTTCCC AAAAGTGGCC
    ATGATCATCA CAGATGGAAA ATCTCAAGAC CCCGTAGAGG AGTACGCCGA ACGGTTGAGG
    AACATCGGAG TGGAGATCTT TGTGCTGGGT ATCAAAGGAG CAGATGAGGA CGAGCTGAAG
    GAGATCGCCT CCAGACCCCA TAGCAAGCAT GTCTATAACG TCCCCAACTT CGACATGATC
    AATCAGGTCC AGAAGGCGCT CATCACTGAA GTTTGCTCTG GCGTGGAGGA GCAGCTAAAT
    TCCCTTGTAA GCGGGGAGGA AGTTGTCGAA CCAGCTTCCA ACCTCAGAGT GACAGATATC
    TCCTCCAAAT CCATGAGAAT TGCGTGGGAT TCATCTCCTG GTGATATCAC AGGCTATAAG
    CTGCAGGTTT TTCCCACTAT TGCGAGCGCC AAAAAGCAGG AGCTCTACAC TGGGCCCACG
    ATGACCTTGG CCAATGTCAG AGACCTGACG CCTGAGATGG AGTATGAGAT CAGTCTGTTT
    GCTCTCAAAG GTCTGACTCC TAGTGTGCCG CAAGTTGTCA CTGAGAAAAC TCAGCCAGTG
    AAAGTATTTA CAGAGTGTTC CCTAGAGGTG GATGTCCAAG CTGACATTGT CCTCCTGGTG
    GATGGGTCAT ATAGTATAGG ACTGCCTAAC TTTGCGAAGG TTAGAGCTTT CCTCGAAGTA
    CTTGTGAATT CTTTTGACAT CGGACCTAAC AAAGTGCAGA TCAGCTTGGT TCAGTACAGC
    AGGGATCCTC ACACTGAGTT CGCCTTGAAT AAATTCGATG ACAATGCAGC CATGGTCAAG
    GCGGTCCGCA CCTTCCCCTA CCGAGGTGGA TCCACCAACA CCGGCAAGGC CATGACATAT
    GTGAGGGAAA AGATCTTTGT TTCGGGCCGT GGCGCTCGTG ATAACGTCCC CCGAGTCATG
    GTGCTTATCA CAGACGGCAA GTCCTCAGAT TCTTTCAAAG ACCCCGCCAA CAAACTGAGG
    GATACAGATG TGGAGATTTT TGCAGTGGGA GTAAAAGATG CCGTACGATC TGAGCTGGAG
    GCTATCGCCA ATGTTCCTGC TGATAACCAT GTGTTTGAGG TGGAGGACTT TGATGCTTTC
    GAGCGGATCT CCAAGGAGCT GACCGCGTCC ATCTGCCTCA GAATTGAGCA GGAGCTCCAT
    AACATCAGGA AAAGAAGTTT GCTTCCCCCA AAGTCACTGA GCTTCTCTGA GGTGACCTCC
    AGGAGTTTCA GGACCTCATG GGTTTCTGAT GGAGAGGATA TCCTGTCTTT TCTGGTGCGC
    TTCAGGCCAG CGGCGGACCT AACTGGAGAC TACATCTCTC TGGTGGTTCC CCATGACATC
    ACCAGTACAA TTCTGCCCAA TCTCACGCCA CTCACCACCT ACGAAGTTAA CGTCATTGCT
    CAGTATGATA AAGGAGACAG CTTTCCTTTG ACGGGCGAGG AGACCACTTT AGAAGAGTTG
    GGTTCTGTTC GTAACCTGAT GGTGAGCGAG GAGGCTGTGG ACCGCTTTCG GGTGTCGTGG
    AGAGCTGCTC CAGGATTAGT GCTGAGGTAC AGACTGCTCT ACGAGCCTGT GAGCGGAGGG
    GATAAACTGG AAGCAGAGAC TGATGGGAAT CAGGTCACCA CTGTGCTCAA TGAACTACTA
    CCGGTCACTA CTTACCGGGT CACCGTTTAC CCTGAGTACG CAAGTGGAAG GGGTCCCTCC
    ATGGACACTG AAGGCACCAC CAAAGAAGTT CGAGGTTCTC CACGCGACTT AAGGGTCTTC
    AATGAGACGG TGTCTAGCAT GAGGGTCTCG TGGCGGGCTG CCCCAGGCAA CGTGCTTCAA
    TATCAGGTGG CCTACAAACC TGACGGTGGG GAGAGGAAAG AAATAAGTGT TAAAGGAGAG
    ACAACAACTG CTGTGCTGAA AAATCTGCTG CCGGACACGG AGTATGAGCT GTTTGTTAGC
    GCACGTTACG TCTCTGGACT TGGCGCACCA CTTATGGGAA CAGGCACCAC GCTGGAAGAA
    CTTGGTTCAC CTTCGAATCT GGTAACTTCA GACGTCACTG AGAACAGTTT TGTAGCATCC
    TGGACCGCCG CCCCTGGAAA CGTCCGTCTT TATCGGGTCA CCTGGAAATC CCTCTTTTCA
    GAGGAAGCTG GCGAGAAGAC TGTCCCCGGG GACGTAACCA CCACTGTTCT GGACGGGCTG
    ACACCAGAGA CCCGCTACAA GGTTTCTGTC TACGCTGTCT ACAGCCGTGC GGAGGGGGCG
    CCACTGCACG GAGAGGAGAC CACAGATGTG TCGGCCGCTA CCAGAGCTCT TACGGTGTCA
    GAAGAAACAG AGAGGACCAT GAAGGTGACA TGGATACCAG CACCTGGCAA TGTTGTCAAT
    TACAGGGTAT CATACAAACC TCAGGGTGAT TTACGCCAAA TGGCCTCCAA AGCACCAGGA
    GGCACCAATT CTATAGTCCT GAGACGCCTG ATCCCCCAGA CCACTTACGA TATCACAGTG
    GTTCCCGTCT ACAAGTTTGG GGATGGAAGA CAAAGGCAAG GAGAAGGAAC TACATTGTCT
    CAATATAAAG CTCCCAGAAA CTTGCAAACT TCGGAGCCCA CTAAAACCAG TTTCAGGGTG
    ACCTGGGATC ATGCTCCTGG AGATGTAAAA GGCTACAAGG TCACTTTCCA TCCCACCGGA
    AAGGAAGTGG ACCTGGGAGA GTTACTCGTA GGCCCCTATG ACAACACTGT GGTTCTGGAG
    GAACTAAGGG CCGGCACTAA GTATACGGTA AATGTGTTTG GTATGTTTGA TGGAGGAGAG
    AGTATGCCAT TGGCTGGAGA GGAGAAGACC ACCCTCTCTG ACGCAGCTTC TGAAAACCTC
    CCTTATACAC CTTCAGATGC CCAGTGTAAA ACCACGGCCC AAGCGGATAT CGTCCTGCTT
    GTTGATGGAT CTTGGAGCAT TGGTCGCCTG AACTTCAAGA CCATACGCAA TTTCATTGCT
    CGCATGGTGG GAGTCTTCGA CATCGGCCCC GATCGTGTCC AGATTGGTCT GGCACAGTAC
    AGCGGAGACC CGAAGACTGA GTGGCATCTC AATGCTCACA GAACCAGGAC TCAGCTGCTG
    GATGCGGTGG CCAATCTACC CTACAAAGGA GGAAACACTC TCACCGGCCT TGCTTTGAAT
    TACATCCTTC AGAATAACTT CAAGCCTAAC GTTGGCATGC GTCCAAACTC CAGAAAGATC
    GGCGTCCTGG TCACTGATGG CAAGTCCCAA GACGACATTG TGGCGAACTC ACAGAACCTG
    CGGGACCAAG GGATTGAACT GTACGCCATT GGTGTAAAGA ACGCAGATGA GAATGAGTTG
    AGAACCATTG CTTCCGATCC CGATGACATT CACATGTACA ACGTGGCTGA TTTCTCCTTC
    CTGCTGGACA TTGTGGATGA CCTCACAAAC AACCTGTGCA ACAGCGTCAA AGGTCCAGGA
    GGAGGACCAG AGCCACCCAC TGATCTGACC ACCTCAGAGG TCACACATTA CTCTTTCCGT
    GCCACTTGGG TGGCTCCTGA CGGGCCTGTG GAGAAGTACA GAGTGGAGTA TGTGAGCACC
    GCTGGAGGAG AACCACAGCA ACAGATGTTT GTGGACGGGA CGGTGACCAC TGTGGTTTTG
    GAAGGTCTCA CTCCGCTGAC CGAGTACATA GTAAAGGTTT ACTCAGTGGT GGGAGAGGAC
    AGCAGCGACC CACTCAGAGG CATAGAGACC ACATTGCCTC TCAGTGCTGT GAGGAGCATG
    AACATCTACA AAGAGCGCTC CACCACCATG AATGTGAGAT GGGACGCAGC CACAGGGGCC
    ACAGGCTACA TGCTTCTCTA CAAGGCCCTT AACGCCACTG AACCCTCTGT GGAGCAGGAG
    ATGCGAGTGA AAGGAATGGT CACTGACGTC CAGCTGAAGA AACTTCTCCC CAACACGGCC
    TACAGTGTTT CGCTCTTCGC CCTGCACGGC GATTACGCCA GTGAACCTCT GATTGATCAA
    GGGGTGACCC TGCCTCTTCC TCCATCCGGT GAGTTGAGGA TTACCGATGT GACCCACAGC
    ACCATGCAGC TGGACTGGGA CGCTGCGCCC GGCGCCGTCC GAAAGTACAT CATCACTTAC
    AAGCCAGAGG AGGGAGAGGC GAAGGAGCTT GAAGTTGACG GAGATGTAAC ATCTAGAGGC
    CTGGATAATC TCATCTCCCA GACTGAATAT GATGTGGCTG TGACTCCGGT TTATGATTCG
    GGCACTGGAA ACCCCATGCT TAACCAGGCC ATTACAGATG TGGTTCCTGC CCCGCAGAAC
    TTGAGGTTCT CGATGGTGAC CCAGACCAGT TTTAGGGTTC ACTGGGAGCA CGGCGCTCCT
    GATGTCTCCC TCTACCGTAT TGGCTGGAAA AAGAAAGGAA CGACCGAGTT TCAAAATGAT
    ATCCTGAGTA GTGAAGACAC TACATATGAT CTGGTGGACC TGGAGCCAGA CACACCTTAT
    GATGTTTCGG TCACTGCCAT TTACCCTGAT GAGTCTGAGA GCGAGGATCT GCTGGGCACT
    GAACGCACTT TGGCCAAGCT TGCTACACCA CCTCCCAGTG GTCCTCCTCA AAACCTCCAG
    GTGTTCGACG AGACCACCAC CTCCCTCACG ACTAAGTGGG ACCACGCGCC TGGACGAGTT
    CAGAACTACA AAATCACCTA CGTACCGCTT ACTGGAGGAC GATCACAGTC GACGCAGGTC
    GGAGGAAAGA AAAACACAGT GATCCTGCAG AAACTGACTC CAGACACTCC TTACTCCATC
    ACTGTTGCTG CGGTCTACGC CAGCGGAGAG GCCAGAGACA TTTCTGGCCA AGGAAAGACA
    TTGCCGCTGG GCAGTGTCAG GAATCTTCAG GTCACTGACC CCACAGTTAG CACTCTGAAT
    GTGCGCTGGG AGCCGGCGGA GGGAAGCGTT CGCCAGTACA GGATCTACTA CCAGCCCGTC
    GCAGGGGGCG CAGAGGACAT GGTCCAGGTT CCTGGAAGCA CCACCAGCAC CGTTCTGAAG
    AACCTGCAAT CTGACACTCA GTACAGAGTG ACGGTGGTGC CAGTCTACGC TCCTGGAGAG
    GGCAAGCGCA TGTCGGAGAC CGGAAAAACA CTGGTTCGGA CCCCTGCCAA GAACATCCAG
    GTTTACAACC CCACCACAAA CACCCTGAAC GTGCGCTGGG AGCCGGCCTC AGGACAGGTG
    CAGCAGTACC GCGTGGCTTA CGCCCCTCTC TCCGGGATCC GACCCTCTCA GTCGGTGCTG
    GTTCCAGGAA ACACCCACAC GGCTCTGCTG CAGCAGCTGG AATCAGACAC TGACTACTCT
    GTGAGTGTGG TGGCTCTGTA TGCTGATGGA GAGGGCTCGT CTGTGTCTGA TAATGGCAAA
    ACACTGCCCC GCAGTGGCCC CAGGAACCTG CGGGTTTATG ATCCCACCAC CAGCACGCTG
    TCGGTGGGCT GGGAACATGC AGAAGGGCCT GTGATTCAGT ACCGCATTGG CTACGCACCC
    ACCACTGGAG ATCCCATTGA AGAATTTACA ACTGTCCCAG GCAACCGTAA CTCGGTCATC
    CTGCAAAACC TGCAGCCAGA CACGCCGTAC AACATCAACG TAGAAGCCAT TTATCAAGAT
    GGCCCTGGAG GACGACTCAG TGGAGATGGA AAAACATTGG ATCTCCTTGG GCCTCGTAAC
    CTGCGTGTTT CAGACGAGTG GTACACACGT TTCCGAGTGT CCTGGGATCC TGCAGCCGCA
    CGGGTGAATG GATATAAACT GGTCTATACA CCAGTGGGCA CAACCGACAC CATGGAAGCC
    TTCGTTGGAG ACGTGACTTC ACACCACCTG AACAACCTCC AGCCTGGCAC CACATACGAC
    CTGAAGGTTT ACGCTCAGTA TGATGCGGGC TTGAGCGGCG CTCTGGTCGG ACAGGGAACC
    ACCCTGTATC TCAACGTGAC CGACCTCACT TCCTACAACG TGGGCTACGA CTCTTTCTGC
    ATCAGATGGA CTCCTCATCG AGCTGCCACC TCCTACAGAC TCAAAGTCAA CCCTTTCAAC
    CCATCTCACC GTGGAGCTCA GGAGGTCACG ATCACTGCGG CTGAGAGCCA GTACTGTTTT
    GACGGCCTGA CGCCAGACGC GCTATACAAC GCCACCGTCT ACACGCAGAC CCCCAACCGA
    GAGGGGCCCG GAGTCAGTGT CAAAGAGCGC ACACTGGTGA AGCCCACCGT CGCCCCAACA
    CTACCTCCAA CTCCTCCACC TCCAGCCACC ATTCCTTCTG CACTCGAGGT GTGCAAAGGT
    GCCAAGGCTG ACCTGGTGTT CCTGATCGAT GGCTCGTGGA GTATCGGAGA TGACAGCTTC
    GCTAAAGTCC GGCAGTTTGT GTTCAGCATG ATTGGGGCGT TTGATGTGGT CAGCCATGAG
    GGCATGCAGG TGTCTTTCGT GCAGTACAGC GACGACGCCA AAACTGAGTT CAAGCTCAAC
    ACCTACCACG ACAAGGGAAT GGTGCTGTCT GCTCTTCAGA TGGTCAATTA TAGAGGAGGA
    AACACCAAAA CAGGTGCTGC ACTGAAGCAT GTTGGAGAAA AAGTGTTCAC CTCGGACAAT
    GGCATGAGGA GAACCGTTCC CAAGGTACTG GTTGTGGTGA CCGATGGACG TTCTCAAGAT
    GACGTGAAGA AGAGCGCTGC TAAACTACAG CATGCAGGTT ACAGTGTGTT TGTGGTGGGT
    GTCGCTGACA TTGACGTTAA TGAACTGAGG AACATTGGAA GCAAGCCCAG TGACAGACAC
    GTCTTTATCG TCGACAACTT CGATGCTTTC GCCAAGATCC AAGACAACCT GATCACGTTC
    ATCTGTGAAA CGGCAACGTC CACATGCCCT TTGATTTACC TCAATGGATT CACATCACCA
    GGCTTCAGGA TGCTGGAAGC TTTCAACATC ACTGACAAGA CCTACGCAGG CATGAACGGC
    GTCTCTCTGG AAACCGGCTC CTTCAACAGC TTCATCGCAT ACAGACTCCA TAAGGACGCC
    TTCATTTCCC AGCCAACAAA GGAAATCCAT CCAGATGGTC TTCCTCCATC CTACACCCTC
    ATCCTCCTCT TCCGTCTGCT CCCGGACACC GGCAAGGATG CGTTTGATTT ATGGCAGATC
    TCAGATAAGA ACAATAACCC TGAGGTCGGG CTCACCATTG ACCCCTCCAG CAGAGTGATC
    TCCTTCTACA ATAAAGACAC TAGAGGGGAA ATTCAGAAAG TCTCCTTTGA TAACGACCAC
    GTGAAGAAGA TTTTCCACGG CAGTTTCCAC AAGCTTCACA TTTCTATTTC GCCGAAAAAT
    GTCAAACTCA ACATCGACTG CCAAGAGGTG GCTGAGAAAC AGATCAAAGC AGCCAACAAC
    GTCTCCACAG ATGGCTACGA AGTCCTTGGA AAAATGACAA AGTCTGGCGG TTCAAAGAAG
    CAGTCGGCCA CATTCCAGCT CCAGATGTTT GATATTGTCT GCAGTTTGGG CTGGACAAGT
    CGAGACAAAT GTTGTGATAT TCCAGCAATG CGAGATGAAG CCAAGTGTCC TGCTCTTCCT
    CACTCCTGCA CATGTGCTCA GGACAGTGTT GGTCCTCCAG GCCCAGCGGG TCCTCAAGGC
    AGTCCTGGTA CAAAAGGCCC ACGAGGCGAG AGAGGAGAGT CTGGTCCTAC GGGTCCAATG
    GGTCCACGTG GAGAGCAGGG TCTGCCAGGG CAGATGGGGC CACCGGGTCC ACAGGGCCCC
    AATGGACTAT CCCTTCCCGG AGAACCAGGG CGACCGGGAC CTAAGGGAGA CCCCGGTGAC
    TCAGGTTTAC CTGGACAGAG AGGCCCGGTT GGCGCTGCAG GTCCACTCGG CCCTGTTGGA
    CCCGCTGGAG CAAGAGGTCC ACCAGGAAGT GAAGGCCCAT CTGGACCAAG AGGTCCATCG
    GGACCAATGG GACCACCGGG AGCTCCCGGA ATGCCAGGAA TCACCGGAAA ACCAGGAAAA
    CCCGGAGACA CTGGCTTTCC TGGACCTGTT GGCATGAAAG GAGAGAAGGG TGAAAGAGGA
    GACACTGCGT CACAGAATAT GATGCGCTCT GTAGCGAGAC AAGTCTGCGA ACAGCTAGTC
    AGCAGACAAA TGAGCAGAAT TGATATGATG ATGAACCAGA TCCCCAGCAA CTACAGGAGT
    AACAGCCCCG GCCCCGCTGG ACCTCCAGGC CCACCTGGAA ACCAGGGACC CCATGGAGAG
    CCTGGTCAGC CCGGACCCAA TGGCTTCCCA GGAAATCCAG GTTTACCAGG CTCTCCTGGA
    GAAAGAGGAG TGCCTGGAGA GAAGGGTGAA CGAGGCAGCC CCGGCATTGG CACTCGAGGA
    CAGAGAGGAT TACCTGGACC TCCTGGGCCA CCAGGGCAGT CTCAGACCGG TCCCCCTGGT
    CCCTCAGGCT CACCCGGGCC CCGAGGACCA CCAGGACAAC ATGGTTCTTC TGGAGTCACA
    GGACCACCAG GACCTCCAGG ATACTGTGAC TCCTCTCAGT GCGGCATGCC TTACAACGGA
    CAAGGCTACC CAGGTTTTGC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa22912
    Clone ID Related Accession (Same CDS sequence) XM_002665259.6 , XM_002665259.5
    Accession Version XM_002665259.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9204bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product collagen alpha-1(XII) chain isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_002665259.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
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    3601
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    3721
    3781
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    3901
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    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
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    4621
    4681
    4741
    4801
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    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
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    5641
    5701
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    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
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    6781
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    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
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    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGAAGATCA GGTTGTCTTT GGCCGCTGCT GCGCTCTTGA CTCTAGTACT CAGTTCAGTA 
    GAGGCTCAAG TGGAGCCTCC GTCAGACTTA AAATTTAAAA TCCTGAATGA AAACACAGTG
    CAAATGTCAT GGAGGAAGCC CTCGTCTCAG ATACAGGGCT ACAGAATACA GGTCACGTCA
    GATACAGATG ATTCTAAAGA ATTGAGTCTT CCTGCCTCGT CCACAAGCAC ATCAATTACA
    GATCTAACTC CAGATGTAGA CTATTCAGTG TCAATCAATT CATATGATGG TGCTGAAGAA
    AGTATCCCTA TCCTTGGACA AATCACAATT CAATCCAGCA ACACAAGTGG ACCAAGGAGG
    CCACAGTCAG AGGCTGTCAA GTGTTCAGTG AGTGCCGTTG CGGATCTGGT TTTCCTGGTG
    GACGGCTCTT GGAGTGTGGG AAGAGAAAAC TTTAGATTTA TCCGGAGTTT CATTGGGGCC
    ATGGCCGGGG CCTTCGACAT TGAAGAGGAT AAGACCAGAG TGGCTGTGGT TCAGTACAGC
    TCGGACACCA GGACAGAGTT CAGTCTAAAC ACACATTTCA GACGACCCGA TGTCCTGCGA
    GCCATTAAAA ATCTGCCGTA CAAGGGTGGA AATACCATGA CGGGTGATGC AATGGACTAT
    CTGGTGAAAA ACACGTTCAC TCAGGCGGCT GGAGCCAGGA AAGGCTTCCC AAAAGTGGCC
    ATGATCATCA CAGATGGAAA ATCTCAAGAC CCCGTAGAGG AGTACGCCGA ACGGTTGAGG
    AACATCGGAG TGGAGATCTT TGTGCTGGGT ATCAAAGGAG CAGATGAGGA CGAGCTGAAG
    GAGATCGCCT CCAGACCCCA TAGCAAGCAT GTCTATAACG TCCCCAACTT CGACATGATC
    AATCAGGTCC AGAAGGCGCT CATCACTGAA GTTTGCTCTG GCGTGGAGGA GCAGCTAAAT
    TCCCTTGTAA GCGGGGAGGA AGTTGTCGAA CCAGCTTCCA ACCTCAGAGT GACAGATATC
    TCCTCCAAAT CCATGAGAAT TGCGTGGGAT TCATCTCCTG GTGATATCAC AGGCTATAAG
    CTGCAGGTTT TTCCCACTAT TGCGAGCGCC AAAAAGCAGG AGCTCTACAC TGGGCCCACG
    ATGACCTTGG CCAATGTCAG AGACCTGACG CCTGAGATGG AGTATGAGAT CAGTCTGTTT
    GCTCTCAAAG GTCTGACTCC TAGTGTGCCG CAAGTTGTCA CTGAGAAAAC TCAGCCAGTG
    AAAGTATTTA CAGAGTGTTC CCTAGAGGTG GATGTCCAAG CTGACATTGT CCTCCTGGTG
    GATGGGTCAT ATAGTATAGG ACTGCCTAAC TTTGCGAAGG TTAGAGCTTT CCTCGAAGTA
    CTTGTGAATT CTTTTGACAT CGGACCTAAC AAAGTGCAGA TCAGCTTGGT TCAGTACAGC
    AGGGATCCTC ACACTGAGTT CGCCTTGAAT AAATTCGATG ACAATGCAGC CATGGTCAAG
    GCGGTCCGCA CCTTCCCCTA CCGAGGTGGA TCCACCAACA CCGGCAAGGC CATGACATAT
    GTGAGGGAAA AGATCTTTGT TTCGGGCCGT GGCGCTCGTG ATAACGTCCC CCGAGTCATG
    GTGCTTATCA CAGACGGCAA GTCCTCAGAT TCTTTCAAAG ACCCCGCCAA CAAACTGAGG
    GATACAGATG TGGAGATTTT TGCAGTGGGA GTAAAAGATG CCGTACGATC TGAGCTGGAG
    GCTATCGCCA ATGTTCCTGC TGATAACCAT GTGTTTGAGG TGGAGGACTT TGATGCTTTC
    GAGCGGATCT CCAAGGAGCT GACCGCGTCC ATCTGCCTCA GAATTGAGCA GGAGCTCCAT
    AACATCAGGA AAAGAAGTTT GCTTCCCCCA AAGTCACTGA GCTTCTCTGA GGTGACCTCC
    AGGAGTTTCA GGACCTCATG GGTTTCTGAT GGAGAGGATA TCCTGTCTTT TCTGGTGCGC
    TTCAGGCCAG CGGCGGACCT AACTGGAGAC TACATCTCTC TGGTGGTTCC CCATGACATC
    ACCAGTACAA TTCTGCCCAA TCTCACGCCA CTCACCACCT ACGAAGTTAA CGTCATTGCT
    CAGTATGATA AAGGAGACAG CTTTCCTTTG ACGGGCGAGG AGACCACTTT AGAAGAGTTG
    GGTTCTGTTC GTAACCTGAT GGTGAGCGAG GAGGCTGTGG ACCGCTTTCG GGTGTCGTGG
    AGAGCTGCTC CAGGATTAGT GCTGAGGTAC AGACTGCTCT ACGAGCCTGT GAGCGGAGGG
    GATAAACTGG AAGCAGAGAC TGATGGGAAT CAGGTCACCA CTGTGCTCAA TGAACTACTA
    CCGGTCACTA CTTACCGGGT CACCGTTTAC CCTGAGTACG CAAGTGGAAG GGGTCCCTCC
    ATGGACACTG AAGGCACCAC CAAAGAAGTT CGAGGTTCTC CACGCGACTT AAGGGTCTTC
    AATGAGACGG TGTCTAGCAT GAGGGTCTCG TGGCGGGCTG CCCCAGGCAA CGTGCTTCAA
    TATCAGGTGG CCTACAAACC TGACGGTGGG GAGAGGAAAG AAATAAGTGT TAAAGGAGAG
    ACAACAACTG CTGTGCTGAA AAATCTGCTG CCGGACACGG AGTATGAGCT GTTTGTTAGC
    GCACGTTACG TCTCTGGACT TGGCGCACCA CTTATGGGAA CAGGCACCAC GCTGGAAGAA
    CTTGGTTCAC CTTCGAATCT GGTAACTTCA GACGTCACTG AGAACAGTTT TGTAGCATCC
    TGGACCGCCG CCCCTGGAAA CGTCCGTCTT TATCGGGTCA CCTGGAAATC CCTCTTTTCA
    GAGGAAGCTG GCGAGAAGAC TGTCCCCGGG GACGTAACCA CCACTGTTCT GGACGGGCTG
    ACACCAGAGA CCCGCTACAA GGTTTCTGTC TACGCTGTCT ACAGCCGTGC GGAGGGGGCG
    CCACTGCACG GAGAGGAGAC CACAGATGTG TCGGCCGCTA CCAGAGCTCT TACGGTGTCA
    GAAGAAACAG AGAGGACCAT GAAGGTGACA TGGATACCAG CACCTGGCAA TGTTGTCAAT
    TACAGGGTAT CATACAAACC TCAGGGTGAT TTACGCCAAA TGGCCTCCAA AGCACCAGGA
    GGCACCAATT CTATAGTCCT GAGACGCCTG ATCCCCCAGA CCACTTACGA TATCACAGTG
    GTTCCCGTCT ACAAGTTTGG GGATGGAAGA CAAAGGCAAG GAGAAGGAAC TACATTGTCT
    CAATATAAAG CTCCCAGAAA CTTGCAAACT TCGGAGCCCA CTAAAACCAG TTTCAGGGTG
    ACCTGGGATC ATGCTCCTGG AGATGTAAAA GGCTACAAGG TCACTTTCCA TCCCACCGGA
    AAGGAAGTGG ACCTGGGAGA GTTACTCGTA GGCCCCTATG ACAACACTGT GGTTCTGGAG
    GAACTAAGGG CCGGCACTAA GTATACGGTA AATGTGTTTG GTATGTTTGA TGGAGGAGAG
    AGTATGCCAT TGGCTGGAGA GGAGAAGACC ACCCTCTCTG ACGCAGCTTC TGAAAACCTC
    CCTTATACAC CTTCAGATGC CCAGTGTAAA ACCACGGCCC AAGCGGATAT CGTCCTGCTT
    GTTGATGGAT CTTGGAGCAT TGGTCGCCTG AACTTCAAGA CCATACGCAA TTTCATTGCT
    CGCATGGTGG GAGTCTTCGA CATCGGCCCC GATCGTGTCC AGATTGGTCT GGCACAGTAC
    AGCGGAGACC CGAAGACTGA GTGGCATCTC AATGCTCACA GAACCAGGAC TCAGCTGCTG
    GATGCGGTGG CCAATCTACC CTACAAAGGA GGAAACACTC TCACCGGCCT TGCTTTGAAT
    TACATCCTTC AGAATAACTT CAAGCCTAAC GTTGGCATGC GTCCAAACTC CAGAAAGATC
    GGCGTCCTGG TCACTGATGG CAAGTCCCAA GACGACATTG TGGCGAACTC ACAGAACCTG
    CGGGACCAAG GGATTGAACT GTACGCCATT GGTGTAAAGA ACGCAGATGA GAATGAGTTG
    AGAACCATTG CTTCCGATCC CGATGACATT CACATGTACA ACGTGGCTGA TTTCTCCTTC
    CTGCTGGACA TTGTGGATGA CCTCACAAAC AACCTGTGCA ACAGCGTCAA AGGTCCAGGA
    GGAGGACCAG AGCCACCCAC TGATCTGACC ACCTCAGAGG TCACACATTA CTCTTTCCGT
    GCCACTTGGG TGGCTCCTGA CGGGCCTGTG GAGAAGTACA GAGTGGAGTA TGTGAGCACC
    GCTGGAGGAG AACCACAGCA ACAGATGTTT GTGGACGGGA CGGTGACCAC TGTGGTTTTG
    GAAGGTCTCA CTCCGCTGAC CGAGTACATA GTAAAGGTTT ACTCAGTGGT GGGAGAGGAC
    AGCAGCGACC CACTCAGAGG CATAGAGACC ACATTGCCTC TCAGTGCTGT GAGGAGCATG
    AACATCTACA AAGAGCGCTC CACCACCATG AATGTGAGAT GGGACGCAGC CACAGGGGCC
    ACAGGCTACA TGCTTCTCTA CAAGGCCCTT AACGCCACTG AACCCTCTGT GGAGCAGGAG
    ATGCGAGTGA AAGGAATGGT CACTGACGTC CAGCTGAAGA AACTTCTCCC CAACACGGCC
    TACAGTGTTT CGCTCTTCGC CCTGCACGGC GATTACGCCA GTGAACCTCT GATTGATCAA
    GGGGTGACCC TGCCTCTTCC TCCATCCGGT GAGTTGAGGA TTACCGATGT GACCCACAGC
    ACCATGCAGC TGGACTGGGA CGCTGCGCCC GGCGCCGTCC GAAAGTACAT CATCACTTAC
    AAGCCAGAGG AGGGAGAGGC GAAGGAGCTT GAAGTTGACG GAGATGTAAC ATCTAGAGGC
    CTGGATAATC TCATCTCCCA GACTGAATAT GATGTGGCTG TGACTCCGGT TTATGATTCG
    GGCACTGGAA ACCCCATGCT TAACCAGGCC ATTACAGATG TGGTTCCTGC CCCGCAGAAC
    TTGAGGTTCT CGATGGTGAC CCAGACCAGT TTTAGGGTTC ACTGGGAGCA CGGCGCTCCT
    GATGTCTCCC TCTACCGTAT TGGCTGGAAA AAGAAAGGAA CGACCGAGTT TCAAAATGAT
    ATCCTGAGTA GTGAAGACAC TACATATGAT CTGGTGGACC TGGAGCCAGA CACACCTTAT
    GATGTTTCGG TCACTGCCAT TTACCCTGAT GAGTCTGAGA GCGAGGATCT GCTGGGCACT
    GAACGCACTT TGGCCAAGCT TGCTACACCA CCTCCCAGTG GTCCTCCTCA AAACCTCCAG
    GTGTTCGACG AGACCACCAC CTCCCTCACG ACTAAGTGGG ACCACGCGCC TGGACGAGTT
    CAGAACTACA AAATCACCTA CGTACCGCTT ACTGGAGGAC GATCACAGTC GACGCAGGTC
    GGAGGAAAGA AAAACACAGT GATCCTGCAG AAACTGACTC CAGACACTCC TTACTCCATC
    ACTGTTGCTG CGGTCTACGC CAGCGGAGAG GCCAGAGACA TTTCTGGCCA AGGAAAGACA
    TTGCCGCTGG GCAGTGTCAG GAATCTTCAG GTCACTGACC CCACAGTTAG CACTCTGAAT
    GTGCGCTGGG AGCCGGCGGA GGGAAGCGTT CGCCAGTACA GGATCTACTA CCAGCCCGTC
    GCAGGGGGCG CAGAGGACAT GGTCCAGGTT CCTGGAAGCA CCACCAGCAC CGTTCTGAAG
    AACCTGCAAT CTGACACTCA GTACAGAGTG ACGGTGGTGC CAGTCTACGC TCCTGGAGAG
    GGCAAGCGCA TGTCGGAGAC CGGAAAAACA CTGGTTCGGA CCCCTGCCAA GAACATCCAG
    GTTTACAACC CCACCACAAA CACCCTGAAC GTGCGCTGGG AGCCGGCCTC AGGACAGGTG
    CAGCAGTACC GCGTGGCTTA CGCCCCTCTC TCCGGGATCC GACCCTCTCA GTCGGTGCTG
    GTTCCAGGAA ACACCCACAC GGCTCTGCTG CAGCAGCTGG AATCAGACAC TGACTACTCT
    GTGAGTGTGG TGGCTCTGTA TGCTGATGGA GAGGGCTCGT CTGTGTCTGA TAATGGCAAA
    ACACTGCCCC GCAGTGGCCC CAGGAACCTG CGGGTTTATG ATCCCACCAC CAGCACGCTG
    TCGGTGGGCT GGGAACATGC AGAAGGGCCT GTGATTCAGT ACCGCATTGG CTACGCACCC
    ACCACTGGAG ATCCCATTGA AGAATTTACA ACTGTCCCAG GCAACCGTAA CTCGGTCATC
    CTGCAAAACC TGCAGCCAGA CACGCCGTAC AACATCAACG TAGAAGCCAT TTATCAAGAT
    GGCCCTGGAG GACGACTCAG TGGAGATGGA AAAACATTGG ATCTCCTTGG GCCTCGTAAC
    CTGCGTGTTT CAGACGAGTG GTACACACGT TTCCGAGTGT CCTGGGATCC TGCAGCCGCA
    CGGGTGAATG GATATAAACT GGTCTATACA CCAGTGGGCA CAACCGACAC CATGGAAGCC
    TTCGTTGGAG ACGTGACTTC ACACCACCTG AACAACCTCC AGCCTGGCAC CACATACGAC
    CTGAAGGTTT ACGCTCAGTA TGATGCGGGC TTGAGCGGCG CTCTGGTCGG ACAGGGAACC
    ACCCTGTATC TCAACGTGAC CGACCTCACT TCCTACAACG TGGGCTACGA CTCTTTCTGC
    ATCAGATGGA CTCCTCATCG AGCTGCCACC TCCTACAGAC TCAAAGTCAA CCCTTTCAAC
    CCATCTCACC GTGGAGCTCA GGAGGTCACG ATCACTGCGG CTGAGAGCCA GTACTGTTTT
    GACGGCCTGA CGCCAGACGC GCTATACAAC GCCACCGTCT ACACGCAGAC CCCCAACCGA
    GAGGGGCCCG GAGTCAGTGT CAAAGAGCGC ACACTGGTGA AGCCCACCGT CGCCCCAACA
    CTACCTCCAA CTCCTCCACC TCCAGCCACC ATTCCTTCTG CACTCGAGGT GTGCAAAGGT
    GCCAAGGCTG ACCTGGTGTT CCTGATCGAT GGCTCGTGGA GTATCGGAGA TGACAGCTTC
    GCTAAAGTCC GGCAGTTTGT GTTCAGCATG ATTGGGGCGT TTGATGTGGT CAGCCATGAG
    GGCATGCAGG TGTCTTTCGT GCAGTACAGC GACGACGCCA AAACTGAGTT CAAGCTCAAC
    ACCTACCACG ACAAGGGAAT GGTGCTGTCT GCTCTTCAGA TGGTCAATTA TAGAGGAGGA
    AACACCAAAA CAGGTGCTGC ACTGAAGCAT GTTGGAGAAA AAGTGTTCAC CTCGGACAAT
    GGCATGAGGA GAACCGTTCC CAAGGTACTG GTTGTGGTGA CCGATGGACG TTCTCAAGAT
    GACGTGAAGA AGAGCGCTGC TAAACTACAG CATGCAGGTT ACAGTGTGTT TGTGGTGGGT
    GTCGCTGACA TTGACGTTAA TGAACTGAGG AACATTGGAA GCAAGCCCAG TGACAGACAC
    GTCTTTATCG TCGACAACTT CGATGCTTTC GCCAAGATCC AAGACAACCT GATCACGTTC
    ATCTGTGAAA CGGCAACGTC CACATGCCCT TTGATTTACC TCAATGGATT CACATCACCA
    GGCTTCAGGA TGCTGGAAGC TTTCAACATC ACTGACAAGA CCTACGCAGG CATGAACGGC
    GTCTCTCTGG AAACCGGCTC CTTCAACAGC TTCATCGCAT ACAGACTCCA TAAGGACGCC
    TTCATTTCCC AGCCAACAAA GGAAATCCAT CCAGATGGTC TTCCTCCATC CTACACCCTC
    ATCCTCCTCT TCCGTCTGCT CCCGGACACC GGCAAGGATG CGTTTGATTT ATGGCAGATC
    TCAGATAAGA ACAATAACCC TGAGGTCGGG CTCACCATTG ACCCCTCCAG CAGAGTGATC
    TCCTTCTACA ATAAAGACAC TAGAGGGGAA ATTCAGAAAG TCTCCTTTGA TAACGACCAC
    GTGAAGAAGA TTTTCCACGG CAGTTTCCAC AAGCTTCACA TTTCTATTTC GCCGAAAAAT
    GTCAAACTCA ACATCGACTG CCAAGAGGTG GCTGAGAAAC AGATCAAAGC AGCCAACAAC
    GTCTCCACAG ATGGCTACGA AGTCCTTGGA AAAATGACAA AGTCTGGCGG TTCAAAGAAG
    CAGTCGGCCA CATTCCAGCT CCAGATGTTT GATATTGTCT GCAGTTTGGG CTGGACAAGT
    CGAGACAAAT GTTGTGATAT TCCAGCAATG CGAGATGAAG CCAAGTGTCC TGCTCTTCCT
    CACTCCTGCA CATGTGCTCA GGACAGTGTT GGTCCTCCAG GCCCAGCGGG TCCTCAAGGC
    AGTCCTGGTA CAAAAGGCCC ACGAGGCGAG AGAGGAGAGT CTGGTCCTAC GGGTCCAATG
    GGTCCACGTG GAGAGCAGGG TCTGCCAGGG CAGATGGGGC CACCGGGTCC ACAGGGCCCC
    AATGGACTAT CCCTTCCCGG AGAACCAGGG CGACCGGGAC CTAAGGGAGA CCCCGGTGAC
    TCAGGTTTAC CTGGACAGAG AGGCCCGGTT GGCGCTGCAG GTCCACTCGG CCCTGTTGGA
    CCCGCTGGAG CAAGAGGTCC ACCAGGAAGT GAAGGCCCAT CTGGACCAAG AGGTCCATCG
    GGACCAATGG GACCACCGGG AGCTCCCGGA ATGCCAGGAA TCACCGGAAA ACCAGGAAAA
    CCCGGAGACA CTGGCTTTCC TGGACCTGTT GGCATGAAAG GAGAGAAGGG TGAAAGAGGA
    GACACTGCGT CACAGAATAT GATGCGCTCT GTAGCGAGAC AAGTCTGCGA ACAGCTAGTC
    AGCAGACAAA TGAGCAGAAT TGATATGATG ATGAACCAGA TCCCCAGCAA CTACAGGAGT
    AACAGCCCCG GCCCCGCTGG ACCTCCAGGC CCACCTGGAA ACCAGGGACC CCATGGAGAG
    CCTGGTCAGC CCGGACCCAA TGGCTTCCCA GGAAATCCAG GTTTACCAGG CTCTCCTGGA
    GAAAGAGGAG TGCCTGGAGA GAAGGGTGAA CGAGGCAGCC CCGGCATTGG CACTCGAGGA
    CAGAGAGGAT TACCTGGACC TCCTGGGCCA CCAGGGCAGT CTCAGACCGG TCCCCCTGGT
    CCCTCAGGCT CACCCGGGCC CCGAGGACCA CCAGGACAAC ATGGTTCTTC TGGAGTCACA
    GGACCACCAG GACCTCCAGG ATACTGTGAC TCCTCTCAGT GCGGCATGCC TTACAACGGA
    CAAGGCTACC CAGGTTTTGC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002665305.3
    CDS140..9343
    Translation

    Target ORF information:

    RefSeq Version XM_002665259.5
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio collagen, type XII, alpha 1a (col12a1a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002665259.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGAAGATCA GGTTGTCTTT GGCCGCTGCT GCGCTCTTGA CTCTAGTACT CAGTTCAGTA 
    GAGGCTCAAG TGGAGCCTCC GTCAGACTTA AAATTTAAAA TCCTGAATGA AAACACAGTG
    CAAATGTCAT GGAGGAAGCC CTCGTCTCAG ATACAGGGCT ACAGAATACA GGTCACGTCA
    GATACAGATG ATTCTAAAGA ATTGAGTCTT CCTGCCTCGT CCACAAGCAC ATCAATTACA
    GATCTAACTC CAGATGTAGA CTATTCAGTG TCAATCAATT CATATGATGG TGCTGAAGAA
    AGTATCCCTA TCCTTGGACA AATCACAATT CAATCCAGCA ACACAAGTGG ACCAAGGAGG
    CCACAGTCAG AGGCTGTCAA GTGTTCAGTG AGTGCCGTTG CGGATCTGGT TTTCCTGGTG
    GACGGCTCTT GGAGTGTGGG AAGAGAAAAC TTTAGATTTA TCCGGAGTTT CATTGGGGCC
    ATGGCCGGGG CCTTCGACAT TGAAGAGGAT AAGACCAGAG TGGCTGTGGT TCAGTACAGC
    TCGGACACCA GGACAGAGTT CAGTCTAAAC ACACATTTCA GACGACCCGA TGTCCTGCGA
    GCCATTAAAA ATCTGCCGTA CAAGGGTGGA AATACCATGA CGGGTGATGC AATGGACTAT
    CTGGTGAAAA ACACGTTCAC TCAGGCGGCT GGAGCCAGGA AAGGCTTCCC AAAAGTGGCC
    ATGATCATCA CAGATGGAAA ATCTCAAGAC CCCGTAGAGG AGTACGCCGA ACGGTTGAGG
    AACATCGGAG TGGAGATCTT TGTGCTGGGT ATCAAAGGAG CAGATGAGGA CGAGCTGAAG
    GAGATCGCCT CCAGACCCCA TAGCAAGCAT GTCTATAACG TCCCCAACTT CGACATGATC
    AATCAGGTCC AGAAGGCGCT CATCACTGAA GTTTGCTCTG GCGTGGAGGA GCAGCTAAAT
    TCCCTTGTAA GCGGGGAGGA AGTTGTCGAA CCAGCTTCCA ACCTCAGAGT GACAGATATC
    TCCTCCAAAT CCATGAGAAT TGCGTGGGAT TCATCTCCTG GTGATATCAC AGGCTATAAG
    CTGCAGGTTT TTCCCACTAT TGCGAGCGCC AAAAAGCAGG AGCTCTACAC TGGGCCCACG
    ATGACCTTGG CCAATGTCAG AGACCTGACG CCTGAGATGG AGTATGAGAT CAGTCTGTTT
    GCTCTCAAAG GTCTGACTCC TAGTGTGCCG CAAGTTGTCA CTGAGAAAAC TCAGCCAGTG
    AAAGTATTTA CAGAGTGTTC CCTAGAGGTG GATGTCCAAG CTGACATTGT CCTCCTGGTG
    GATGGGTCAT ATAGTATAGG ACTGCCTAAC TTTGCGAAGG TTAGAGCTTT CCTCGAAGTA
    CTTGTGAATT CTTTTGACAT CGGACCTAAC AAAGTGCAGA TCAGCTTGGT TCAGTACAGC
    AGGGATCCTC ACACTGAGTT CGCCTTGAAT AAATTCGATG ACAATGCAGC CATGGTCAAG
    GCGGTCCGCA CCTTCCCCTA CCGAGGTGGA TCCACCAACA CCGGCAAGGC CATGACATAT
    GTGAGGGAAA AGATCTTTGT TTCGGGCCGT GGCGCTCGTG ATAACGTCCC CCGAGTCATG
    GTGCTTATCA CAGACGGCAA GTCCTCAGAT TCTTTCAAAG ACCCCGCCAA CAAACTGAGG
    GATACAGATG TGGAGATTTT TGCAGTGGGA GTAAAAGATG CCGTACGATC TGAGCTGGAG
    GCTATCGCCA ATGTTCCTGC TGATAACCAT GTGTTTGAGG TGGAGGACTT TGATGCTTTC
    GAGCGGATCT CCAAGGAGCT GACCGCGTCC ATCTGCCTCA GAATTGAGCA GGAGCTCCAT
    AACATCAGGA AAAGAAGTTT GCTTCCCCCA AAGTCACTGA GCTTCTCTGA GGTGACCTCC
    AGGAGTTTCA GGACCTCATG GGTTTCTGAT GGAGAGGATA TCCTGTCTTT TCTGGTGCGC
    TTCAGGCCAG CGGCGGACCT AACTGGAGAC TACATCTCTC TGGTGGTTCC CCATGACATC
    ACCAGTACAA TTCTGCCCAA TCTCACGCCA CTCACCACCT ACGAAGTTAA CGTCATTGCT
    CAGTATGATA AAGGAGACAG CTTTCCTTTG ACGGGCGAGG AGACCACTTT AGAAGAGTTG
    GGTTCTGTTC GTAACCTGAT GGTGAGCGAG GAGGCTGTGG ACCGCTTTCG GGTGTCGTGG
    AGAGCTGCTC CAGGATTAGT GCTGAGGTAC AGACTGCTCT ACGAGCCTGT GAGCGGAGGG
    GATAAACTGG AAGCAGAGAC TGATGGGAAT CAGGTCACCA CTGTGCTCAA TGAACTACTA
    CCGGTCACTA CTTACCGGGT CACCGTTTAC CCTGAGTACG CAAGTGGAAG GGGTCCCTCC
    ATGGACACTG AAGGCACCAC CAAAGAAGTT CGAGGTTCTC CACGCGACTT AAGGGTCTTC
    AATGAGACGG TGTCTAGCAT GAGGGTCTCG TGGCGGGCTG CCCCAGGCAA CGTGCTTCAA
    TATCAGGTGG CCTACAAACC TGACGGTGGG GAGAGGAAAG AAATAAGTGT TAAAGGAGAG
    ACAACAACTG CTGTGCTGAA AAATCTGCTG CCGGACACGG AGTATGAGCT GTTTGTTAGC
    GCACGTTACG TCTCTGGACT TGGCGCACCA CTTATGGGAA CAGGCACCAC GCTGGAAGAA
    CTTGGTTCAC CTTCGAATCT GGTAACTTCA GACGTCACTG AGAACAGTTT TGTAGCATCC
    TGGACCGCCG CCCCTGGAAA CGTCCGTCTT TATCGGGTCA CCTGGAAATC CCTCTTTTCA
    GAGGAAGCTG GCGAGAAGAC TGTCCCCGGG GACGTAACCA CCACTGTTCT GGACGGGCTG
    ACACCAGAGA CCCGCTACAA GGTTTCTGTC TACGCTGTCT ACAGCCGTGC GGAGGGGGCG
    CCACTGCACG GAGAGGAGAC CACAGATGTG TCGGCCGCTA CCAGAGCTCT TACGGTGTCA
    GAAGAAACAG AGAGGACCAT GAAGGTGACA TGGATACCAG CACCTGGCAA TGTTGTCAAT
    TACAGGGTAT CATACAAACC TCAGGGTGAT TTACGCCAAA TGGCCTCCAA AGCACCAGGA
    GGCACCAATT CTATAGTCCT GAGACGCCTG ATCCCCCAGA CCACTTACGA TATCACAGTG
    GTTCCCGTCT ACAAGTTTGG GGATGGAAGA CAAAGGCAAG GAGAAGGAAC TACATTGTCT
    CAATATAAAG CTCCCAGAAA CTTGCAAACT TCGGAGCCCA CTAAAACCAG TTTCAGGGTG
    ACCTGGGATC ATGCTCCTGG AGATGTAAAA GGCTACAAGG TCACTTTCCA TCCCACCGGA
    AAGGAAGTGG ACCTGGGAGA GTTACTCGTA GGCCCCTATG ACAACACTGT GGTTCTGGAG
    GAACTAAGGG CCGGCACTAA GTATACGGTA AATGTGTTTG GTATGTTTGA TGGAGGAGAG
    AGTATGCCAT TGGCTGGAGA GGAGAAGACC ACCCTCTCTG ACGCAGCTTC TGAAAACCTC
    CCTTATACAC CTTCAGATGC CCAGTGTAAA ACCACGGCCC AAGCGGATAT CGTCCTGCTT
    GTTGATGGAT CTTGGAGCAT TGGTCGCCTG AACTTCAAGA CCATACGCAA TTTCATTGCT
    CGCATGGTGG GAGTCTTCGA CATCGGCCCC GATCGTGTCC AGATTGGTCT GGCACAGTAC
    AGCGGAGACC CGAAGACTGA GTGGCATCTC AATGCTCACA GAACCAGGAC TCAGCTGCTG
    GATGCGGTGG CCAATCTACC CTACAAAGGA GGAAACACTC TCACCGGCCT TGCTTTGAAT
    TACATCCTTC AGAATAACTT CAAGCCTAAC GTTGGCATGC GTCCAAACTC CAGAAAGATC
    GGCGTCCTGG TCACTGATGG CAAGTCCCAA GACGACATTG TGGCGAACTC ACAGAACCTG
    CGGGACCAAG GGATTGAACT GTACGCCATT GGTGTAAAGA ACGCAGATGA GAATGAGTTG
    AGAACCATTG CTTCCGATCC CGATGACATT CACATGTACA ACGTGGCTGA TTTCTCCTTC
    CTGCTGGACA TTGTGGATGA CCTCACAAAC AACCTGTGCA ACAGCGTCAA AGGTCCAGGA
    GGAGGACCAG AGCCACCCAC TGATCTGACC ACCTCAGAGG TCACACATTA CTCTTTCCGT
    GCCACTTGGG TGGCTCCTGA CGGGCCTGTG GAGAAGTACA GAGTGGAGTA TGTGAGCACC
    GCTGGAGGAG AACCACAGCA ACAGATGTTT GTGGACGGGA CGGTGACCAC TGTGGTTTTG
    GAAGGTCTCA CTCCGCTGAC CGAGTACATA GTAAAGGTTT ACTCAGTGGT GGGAGAGGAC
    AGCAGCGACC CACTCAGAGG CATAGAGACC ACATTGCCTC TCAGTGCTGT GAGGAGCATG
    AACATCTACA AAGAGCGCTC CACCACCATG AATGTGAGAT GGGACGCAGC CACAGGGGCC
    ACAGGCTACA TGCTTCTCTA CAAGGCCCTT AACGCCACTG AACCCTCTGT GGAGCAGGAG
    ATGCGAGTGA AAGGAATGGT CACTGACGTC CAGCTGAAGA AACTTCTCCC CAACACGGCC
    TACAGTGTTT CGCTCTTCGC CCTGCACGGC GATTACGCCA GTGAACCTCT GATTGATCAA
    GGGGTGACCC TGCCTCTTCC TCCATCCGGT GAGTTGAGGA TTACCGATGT GACCCACAGC
    ACCATGCAGC TGGACTGGGA CGCTGCGCCC GGCGCCGTCC GAAAGTACAT CATCACTTAC
    AAGCCAGAGG AGGGAGAGGC GAAGGAGCTT GAAGTTGACG GAGATGTAAC ATCTAGAGGC
    CTGGATAATC TCATCTCCCA GACTGAATAT GATGTGGCTG TGACTCCGGT TTATGATTCG
    GGCACTGGAA ACCCCATGCT TAACCAGGCC ATTACAGATG TGGTTCCTGC CCCGCAGAAC
    TTGAGGTTCT CGATGGTGAC CCAGACCAGT TTTAGGGTTC ACTGGGAGCA CGGCGCTCCT
    GATGTCTCCC TCTACCGTAT TGGCTGGAAA AAGAAAGGAA CGACCGAGTT TCAAAATGAT
    ATCCTGAGTA GTGAAGACAC TACATATGAT CTGGTGGACC TGGAGCCAGA CACACCTTAT
    GATGTTTCGG TCACTGCCAT TTACCCTGAT GAGTCTGAGA GCGAGGATCT GCTGGGCACT
    GAACGCACTT TGGCCAAGCT TGCTACACCA CCTCCCAGTG GTCCTCCTCA AAACCTCCAG
    GTGTTCGACG AGACCACCAC CTCCCTCACG ACTAAGTGGG ACCACGCGCC TGGACGAGTT
    CAGAACTACA AAATCACCTA CGTACCGCTT ACTGGAGGAC GATCACAGTC GACGCAGGTC
    GGAGGAAAGA AAAACACAGT GATCCTGCAG AAACTGACTC CAGACACTCC TTACTCCATC
    ACTGTTGCTG CGGTCTACGC CAGCGGAGAG GCCAGAGACA TTTCTGGCCA AGGAAAGACA
    TTGCCGCTGG GCAGTGTCAG GAATCTTCAG GTCACTGACC CCACAGTTAG CACTCTGAAT
    GTGCGCTGGG AGCCGGCGGA GGGAAGCGTT CGCCAGTACA GGATCTACTA CCAGCCCGTC
    GCAGGGGGCG CAGAGGACAT GGTCCAGGTT CCTGGAAGCA CCACCAGCAC CGTTCTGAAG
    AACCTGCAAT CTGACACTCA GTACAGAGTG ACGGTGGTGC CAGTCTACGC TCCTGGAGAG
    GGCAAGCGCA TGTCGGAGAC CGGAAAAACA CTGGTTCGGA CCCCTGCCAA GAACATCCAG
    GTTTACAACC CCACCACAAA CACCCTGAAC GTGCGCTGGG AGCCGGCCTC AGGACAGGTG
    CAGCAGTACC GCGTGGCTTA CGCCCCTCTC TCCGGGATCC GACCCTCTCA GTCGGTGCTG
    GTTCCAGGAA ACACCCACAC GGCTCTGCTG CAGCAGCTGG AATCAGACAC TGACTACTCT
    GTGAGTGTGG TGGCTCTGTA TGCTGATGGA GAGGGCTCGT CTGTGTCTGA TAATGGCAAA
    ACACTGCCCC GCAGTGGCCC CAGGAACCTG CGGGTTTATG ATCCCACCAC CAGCACGCTG
    TCGGTGGGCT GGGAACATGC AGAAGGGCCT GTGATTCAGT ACCGCATTGG CTACGCACCC
    ACCACTGGAG ATCCCATTGA AGAATTTACA ACTGTCCCAG GCAACCGTAA CTCGGTCATC
    CTGCAAAACC TGCAGCCAGA CACGCCGTAC AACATCAACG TAGAAGCCAT TTATCAAGAT
    GGCCCTGGAG GACGACTCAG TGGAGATGGA AAAACATTGG ATCTCCTTGG GCCTCGTAAC
    CTGCGTGTTT CAGACGAGTG GTACACACGT TTCCGAGTGT CCTGGGATCC TGCAGCCGCA
    CGGGTGAATG GATATAAACT GGTCTATACA CCAGTGGGCA CAACCGACAC CATGGAAGCC
    TTCGTTGGAG ACGTGACTTC ACACCACCTG AACAACCTCC AGCCTGGCAC CACATACGAC
    CTGAAGGTTT ACGCTCAGTA TGATGCGGGC TTGAGCGGCG CTCTGGTCGG ACAGGGAACC
    ACCCTGTATC TCAACGTGAC CGACCTCACT TCCTACAACG TGGGCTACGA CTCTTTCTGC
    ATCAGATGGA CTCCTCATCG AGCTGCCACC TCCTACAGAC TCAAAGTCAA CCCTTTCAAC
    CCATCTCACC GTGGAGCTCA GGAGGTCACG ATCACTGCGG CTGAGAGCCA GTACTGTTTT
    GACGGCCTGA CGCCAGACGC GCTATACAAC GCCACCGTCT ACACGCAGAC CCCCAACCGA
    GAGGGGCCCG GAGTCAGTGT CAAAGAGCGC ACACTGGTGA AGCCCACCGT CGCCCCAACA
    CTACCTCCAA CTCCTCCACC TCCAGCCACC ATTCCTTCTG CACTCGAGGT GTGCAAAGGT
    GCCAAGGCTG ACCTGGTGTT CCTGATCGAT GGCTCGTGGA GTATCGGAGA TGACAGCTTC
    GCTAAAGTCC GGCAGTTTGT GTTCAGCATG ATTGGGGCGT TTGATGTGGT CAGCCATGAG
    GGCATGCAGG TGTCTTTCGT GCAGTACAGC GACGACGCCA AAACTGAGTT CAAGCTCAAC
    ACCTACCACG ACAAGGGAAT GGTGCTGTCT GCTCTTCAGA TGGTCAATTA TAGAGGAGGA
    AACACCAAAA CAGGTGCTGC ACTGAAGCAT GTTGGAGAAA AAGTGTTCAC CTCGGACAAT
    GGCATGAGGA GAACCGTTCC CAAGGTACTG GTTGTGGTGA CCGATGGACG TTCTCAAGAT
    GACGTGAAGA AGAGCGCTGC TAAACTACAG CATGCAGGTT ACAGTGTGTT TGTGGTGGGT
    GTCGCTGACA TTGACGTTAA TGAACTGAGG AACATTGGAA GCAAGCCCAG TGACAGACAC
    GTCTTTATCG TCGACAACTT CGATGCTTTC GCCAAGATCC AAGACAACCT GATCACGTTC
    ATCTGTGAAA CGGCAACGTC CACATGCCCT TTGATTTACC TCAATGGATT CACATCACCA
    GGCTTCAGGA TGCTGGAAGC TTTCAACATC ACTGACAAGA CCTACGCAGG CATGAACGGC
    GTCTCTCTGG AAACCGGCTC CTTCAACAGC TTCATCGCAT ACAGACTCCA TAAGGACGCC
    TTCATTTCCC AGCCAACAAA GGAAATCCAT CCAGATGGTC TTCCTCCATC CTACACCCTC
    ATCCTCCTCT TCCGTCTGCT CCCGGACACC GGCAAGGATG CGTTTGATTT ATGGCAGATC
    TCAGATAAGA ACAATAACCC TGAGGTCGGG CTCACCATTG ACCCCTCCAG CAGAGTGATC
    TCCTTCTACA ATAAAGACAC TAGAGGGGAA ATTCAGAAAG TCTCCTTTGA TAACGACCAC
    GTGAAGAAGA TTTTCCACGG CAGTTTCCAC AAGCTTCACA TTTCTATTTC GCCGAAAAAT
    GTCAAACTCA ACATCGACTG CCAAGAGGTG GCTGAGAAAC AGATCAAAGC AGCCAACAAC
    GTCTCCACAG ATGGCTACGA AGTCCTTGGA AAAATGACAA AGTCTGGCGG TTCAAAGAAG
    CAGTCGGCCA CATTCCAGCT CCAGATGTTT GATATTGTCT GCAGTTTGGG CTGGACAAGT
    CGAGACAAAT GTTGTGATAT TCCAGCAATG CGAGATGAAG CCAAGTGTCC TGCTCTTCCT
    CACTCCTGCA CATGTGCTCA GGACAGTGTT GGTCCTCCAG GCCCAGCGGG TCCTCAAGGC
    AGTCCTGGTA CAAAAGGCCC ACGAGGCGAG AGAGGAGAGT CTGGTCCTAC GGGTCCAATG
    GGTCCACGTG GAGAGCAGGG TCTGCCAGGG CAGATGGGGC CACCGGGTCC ACAGGGCCCC
    AATGGACTAT CCCTTCCCGG AGAACCAGGG CGACCGGGAC CTAAGGGAGA CCCCGGTGAC
    TCAGGTTTAC CTGGACAGAG AGGCCCGGTT GGCGCTGCAG GTCCACTCGG CCCTGTTGGA
    CCCGCTGGAG CAAGAGGTCC ACCAGGAAGT GAAGGCCCAT CTGGACCAAG AGGTCCATCG
    GGACCAATGG GACCACCGGG AGCTCCCGGA ATGCCAGGAA TCACCGGAAA ACCAGGAAAA
    CCCGGAGACA CTGGCTTTCC TGGACCTGTT GGCATGAAAG GAGAGAAGGG TGAAAGAGGA
    GACACTGCGT CACAGAATAT GATGCGCTCT GTAGCGAGAC AAGTCTGCGA ACAGCTAGTC
    AGCAGACAAA TGAGCAGAAT TGATATGATG ATGAACCAGA TCCCCAGCAA CTACAGGAGT
    AACAGCCCCG GCCCCGCTGG ACCTCCAGGC CCACCTGGAA ACCAGGGACC CCATGGAGAG
    CCTGGTCAGC CCGGACCCAA TGGCTTCCCA GGAAATCCAG GTTTACCAGG CTCTCCTGGA
    GAAAGAGGAG TGCCTGGAGA GAAGGGTGAA CGAGGCAGCC CCGGCATTGG CACTCGAGGA
    CAGAGAGGAT TACCTGGACC TCCTGGGCCA CCAGGGCAGT CTCAGACCGG TCCCCCTGGT
    CCCTCAGGCT CACCCGGGCC CCGAGGACCA CCAGGACAAC ATGGTTCTTC TGGAGTCACA
    GGACCACCAG GACCTCCAGG ATACTGTGAC TCCTCTCAGT GCGGCATGCC TTACAACGGA
    CAAGGCTACC CAGGTTTTGC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48772
    Clone ID Related Accession (Same CDS sequence) XM_009293296.2 , XM_009293296.3
    Accession Version XM_009293296.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9339bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product collagen alpha-1(XII) chain isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293296.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    ATGAAGATCA GGTTGTCTTT GGCCGCTGCT GCGCTCTTGA CTCTAGTACT CAGTTCAGTA 
    GAGGCTCAAG TGGAGCCTCC GTCAGACTTA AAATTTAAAA TCCTGAATGA AAACACAGTG
    CAAATGTCAT GGAGGAAGCC CTCGTCTCAG ATACAGGGCT ACAGAATACA GGTCACGTCA
    GATACAGATG ATTCTAAAGA ATTGAGTCTT CCTGCCTCGT CCACAAGCAC ATCAATTACA
    GATCTAACTC CAGATGTAGA CTATTCAGTG TCAATCAATT CATATGATGG TGCTGAAGAA
    AGTATCCCTA TCCTTGGACA AATCACAATT CAATCCAGCA ACACAAGTGG ACCAAGGAGG
    CCACAGTCAG AGGCTGTCAA GTGTTCAGTG AGTGCCGTTG CGGATCTGGT TTTCCTGGTG
    GACGGCTCTT GGAGTGTGGG AAGAGAAAAC TTTAGATTTA TCCGGAGTTT CATTGGGGCC
    ATGGCCGGGG CCTTCGACAT TGAAGAGGAT AAGACCAGAG TGGCTGTGGT TCAGTACAGC
    TCGGACACCA GGACAGAGTT CAGTCTAAAC ACACATTTCA GACGACCCGA TGTCCTGCGA
    GCCATTAAAA ATCTGCCGTA CAAGGGTGGA AATACCATGA CGGGTGATGC AATGGACTAT
    CTGGTGAAAA ACACGTTCAC TCAGGCGGCT GGAGCCAGGA AAGGCTTCCC AAAAGTGGCC
    ATGATCATCA CAGATGGAAA ATCTCAAGAC CCCGTAGAGG AGTACGCCGA ACGGTTGAGG
    AACATCGGAG TGGAGATCTT TGTGCTGGGT ATCAAAGGAG CAGATGAGGA CGAGCTGAAG
    GAGATCGCCT CCAGACCCCA TAGCAAGCAT GTCTATAACG TCCCCAACTT CGACATGATC
    AATCAGGTCC AGAAGGCGCT CATCACTGAA GTTTGCTCTG GCGTGGAGGA GCAGCTAAAT
    TCCCTTGTAA GCGGGGAGGA AGTTGTCGAA CCAGCTTCCA ACCTCAGAGT GACAGATATC
    TCCTCCAAAT CCATGAGAAT TGCGTGGGAT TCATCTCCTG GTGATATCAC AGGCTATAAG
    CTGCAGGTTT TTCCCACTAT TGCGAGCGCC AAAAAGCAGG AGCTCTACAC TGGGCCCACG
    ATGACCTTGG CCAATGTCAG AGACCTGACG CCTGAGATGG AGTATGAGAT CAGTCTGTTT
    GCTCTCAAAG GTCTGACTCC TAGTGTGCCG CAAGTTGTCA CTGAGAAAAC TCAGCCAGTG
    AAAGTATTTA CAGAGTGTTC CCTAGAGGTG GATGTCCAAG CTGACATTGT CCTCCTGGTG
    GATGGGTCAT ATAGTATAGG ACTGCCTAAC TTTGCGAAGG TTAGAGCTTT CCTCGAAGTA
    CTTGTGAATT CTTTTGACAT CGGACCTAAC AAAGTGCAGA TCAGCTTGGT TCAGTACAGC
    AGGGATCCTC ACACTGAGTT CGCCTTGAAT AAATTCGATG ACAATGCAGC CATGGTCAAG
    GCGGTCCGCA CCTTCCCCTA CCGAGGTGGA TCCACCAACA CCGGCAAGGC CATGACATAT
    GTGAGGGAAA AGATCTTTGT TTCGGGCCGT GGCGCTCGTG ATAACGTCCC CCGAGTCATG
    GTGCTTATCA CAGACGGCAA GTCCTCAGAT TCTTTCAAAG ACCCCGCCAA CAAACTGAGG
    GATACAGATG TGGAGATTTT TGCAGTGGGA GTAAAAGATG CCGTACGATC TGAGCTGGAG
    GCTATCGCCA ATGTTCCTGC TGATAACCAT GTGTTTGAGG TGGAGGACTT TGATGCTTTC
    GAGCGGATCT CCAAGGAGCT GACCGCGTCC ATCTGCCTCA GAATTGAGCA GGAGCTCCAT
    AACATCAGGA AAAGAAGTTT GCTTCCCCCA AAGTCACTGA GCTTCTCTGA GGTGACCTCC
    AGGAGTTTCA GGACCTCATG GGTTTCTGAT GGAGAGGATA TCCTGTCTTT TCTGGTGCGC
    TTCAGGCCAG CGGCGGACCT AACTGGAGAC TACATCTCTC TGGTGGTTCC CCATGACATC
    ACCAGTACAA TTCTGCCCAA TCTCACGCCA CTCACCACCT ACGAAGTTAA CGTCATTGCT
    CAGTATGATA AAGGAGACAG CTTTCCTTTG ACGGGCGAGG AGACCACTTT AGAAGAGTTG
    GGTTCTGTTC GTAACCTGAT GGTGAGCGAG GAGGCTGTGG ACCGCTTTCG GGTGTCGTGG
    AGAGCTGCTC CAGGATTAGT GCTGAGGTAC AGACTGCTCT ACGAGCCTGT GAGCGGAGGG
    GATAAACTGG AAGCAGAGAC TGATGGGAAT CAGGTCACCA CTGTGCTCAA TGAACTACTA
    CCGGTCACTA CTTACCGGGT CACCGTTTAC CCTGAGTACG CAAGTGGAAG GGGTCCCTCC
    ATGGACACTG AAGGCACCAC CAAAGAAGTT CGAGGTTCTC CACGCGACTT AAGGGTCTTC
    AATGAGACGG TGTCTAGCAT GAGGGTCTCG TGGCGGGCTG CCCCAGGCAA CGTGCTTCAA
    TATCAGGTGG CCTACAAACC TGACGGTGGG GAGAGGAAAG AAATAAGTGT TAAAGGAGAG
    ACAACAACTG CTGTGCTGAA AAATCTGCTG CCGGACACGG AGTATGAGCT GTTTGTTAGC
    GCACGTTACG TCTCTGGACT TGGCGCACCA CTTATGGGAA CAGGCACCAC GCTGGAAGAA
    CTTGGTTCAC CTTCGAATCT GGTAACTTCA GACGTCACTG AGAACAGTTT TGTAGCATCC
    TGGACCGCCG CCCCTGGAAA CGTCCGTCTT TATCGGGTCA CCTGGAAATC CCTCTTTTCA
    GAGGAAGCTG GCGAGAAGAC TGTCCCCGGG GACGTAACCA CCACTGTTCT GGACGGGCTG
    ACACCAGAGA CCCGCTACAA GGTTTCTGTC TACGCTGTCT ACAGCCGTGC GGAGGGGGCG
    CCACTGCACG GAGAGGAGAC CACAGATGTG TCGGCCGCTA CCAGAGCTCT TACGGTGTCA
    GAAGAAACAG AGAGGACCAT GAAGGTGACA TGGATACCAG CACCTGGCAA TGTTGTCAAT
    TACAGGGTAT CATACAAACC TCAGGGTGAT TTACGCCAAA TGGCCTCCAA AGCACCAGGA
    GGCACCAATT CTATAGTCCT GAGACGCCTG ATCCCCCAGA CCACTTACGA TATCACAGTG
    GTTCCCGTCT ACAAGTTTGG GGATGGAAGA CAAAGGCAAG GAGAAGGAAC TACATTGTCT
    CAATATAAAG CTCCCAGAAA CTTGCAAACT TCGGAGCCCA CTAAAACCAG TTTCAGGGTG
    ACCTGGGATC ATGCTCCTGG AGATGTAAAA GGCTACAAGG TCACTTTCCA TCCCACCGGA
    AAGGAAGTGG ACCTGGGAGA GTTACTCGTA GGCCCCTATG ACAACACTGT GGTTCTGGAG
    GAACTAAGGG CCGGCACTAA GTATACGGTA AATGTGTTTG GTATGTTTGA TGGAGGAGAG
    AGTATGCCAT TGGCTGGAGA GGAGAAGACC ACCCTCTCTG ACGCAGCTTC TGAAAACCTC
    CCTTATACAC CTTCAGATGC CCAGTGTAAA ACCACGGCCC AAGCGGATAT CGTCCTGCTT
    GTTGATGGAT CTTGGAGCAT TGGTCGCCTG AACTTCAAGA CCATACGCAA TTTCATTGCT
    CGCATGGTGG GAGTCTTCGA CATCGGCCCC GATCGTGTCC AGATTGGTCT GGCACAGTAC
    AGCGGAGACC CGAAGACTGA GTGGCATCTC AATGCTCACA GAACCAGGAC TCAGCTGCTG
    GATGCGGTGG CCAATCTACC CTACAAAGGA GGAAACACTC TCACCGGCCT TGCTTTGAAT
    TACATCCTTC AGAATAACTT CAAGCCTAAC GTTGGCATGC GTCCAAACTC CAGAAAGATC
    GGCGTCCTGG TCACTGATGG CAAGTCCCAA GACGACATTG TGGCGAACTC ACAGAACCTG
    CGGGACCAAG GGATTGAACT GTACGCCATT GGTGTAAAGA ACGCAGATGA GAATGAGTTG
    AGAACCATTG CTTCCGATCC CGATGACATT CACATGTACA ACGTGGCTGA TTTCTCCTTC
    CTGCTGGACA TTGTGGATGA CCTCACAAAC AACCTGTGCA ACAGCGTCAA AGGTCCAGGA
    GGAGGACCAG AGCCACCCAC TGATCTGACC ACCTCAGAGG TCACACATTA CTCTTTCCGT
    GCCACTTGGG TGGCTCCTGA CGGGCCTGTG GAGAAGTACA GAGTGGAGTA TGTGAGCACC
    GCTGGAGGAG AACCACAGCA ACAGATGTTT GTGGACGGGA CGGTGACCAC TGTGGTTTTG
    GAAGGTCTCA CTCCGCTGAC CGAGTACATA GTAAAGGTTT ACTCAGTGGT GGGAGAGGAC
    AGCAGCGACC CACTCAGAGG CATAGAGACC ACATTGCCTC TCAGTGCTGT GAGGAGCATG
    AACATCTACA AAGAGCGCTC CACCACCATG AATGTGAGAT GGGACGCAGC CACAGGGGCC
    ACAGGCTACA TGCTTCTCTA CAAGGCCCTT AACGCCACTG AACCCTCTGT GGAGCAGGAG
    ATGCGAGTGA AAGGAATGGT CACTGACGTC CAGCTGAAGA AACTTCTCCC CAACACGGCC
    TACAGTGTTT CGCTCTTCGC CCTGCACGGC GATTACGCCA GTGAACCTCT GATTGATCAA
    GGGGTGACCC TGCCTCTTCC TCCATCCGGT GAGTTGAGGA TTACCGATGT GACCCACAGC
    ACCATGCAGC TGGACTGGGA CGCTGCGCCC GGCGCCGTCC GAAAGTACAT CATCACTTAC
    AAGCCAGAGG AGGGAGAGGC GAAGGAGCTT GAAGTTGACG GAGATGTAAC ATCTAGAGGC
    CTGGATAATC TCATCTCCCA GACTGAATAT GATGTGGCTG TGACTCCGGT TTATGATTCG
    GGCACTGGAA ACCCCATGCT TAACCAGGCC ATTACAGATG TGGTTCCTGC CCCGCAGAAC
    TTGAGGTTCT CGATGGTGAC CCAGACCAGT TTTAGGGTTC ACTGGGAGCA CGGCGCTCCT
    GATGTCTCCC TCTACCGTAT TGGCTGGAAA AAGAAAGGAA CGACCGAGTT TCAAAATGAT
    ATCCTGAGTA GTGAAGACAC TACATATGAT CTGGTGGACC TGGAGCCAGA CACACCTTAT
    GATGTTTCGG TCACTGCCAT TTACCCTGAT GAGTCTGAGA GCGAGGATCT GCTGGGCACT
    GAACGCACTT TGGCCAAGCT TGCTACACCA CCTCCCAGTG GTCCTCCTCA AAACCTCCAG
    GTGTTCGACG AGACCACCAC CTCCCTCACG ACTAAGTGGG ACCACGCGCC TGGACGAGTT
    CAGAACTACA AAATCACCTA CGTACCGCTT ACTGGAGGAC GATCACAGTC GACGCAGGTC
    GGAGGAAAGA AAAACACAGT GATCCTGCAG AAACTGACTC CAGACACTCC TTACTCCATC
    ACTGTTGCTG CGGTCTACGC CAGCGGAGAG GCCAGAGACA TTTCTGGCCA AGGAAAGACA
    TTGCCGCTGG GCAGTGTCAG GAATCTTCAG GTCACTGACC CCACAGTTAG CACTCTGAAT
    GTGCGCTGGG AGCCGGCGGA GGGAAGCGTT CGCCAGTACA GGATCTACTA CCAGCCCGTC
    GCAGGGGGCG CAGAGGACAT GGTCCAGGTT CCTGGAAGCA CCACCAGCAC CGTTCTGAAG
    AACCTGCAAT CTGACACTCA GTACAGAGTG ACGGTGGTGC CAGTCTACGC TCCTGGAGAG
    GGCAAGCGCA TGTCGGAGAC CGGAAAAACA CTGGTTCGGA CCCCTGCCAA GAACATCCAG
    GTTTACAACC CCACCACAAA CACCCTGAAC GTGCGCTGGG AGCCGGCCTC AGGACAGGTG
    CAGCAGTACC GCGTGGCTTA CGCCCCTCTC TCCGGGATCC GACCCTCTCA GTCGGTGCTG
    GTTCCAGGAA ACACCCACAC GGCTCTGCTG CAGCAGCTGG AATCAGACAC TGACTACTCT
    GTGAGTGTGG TGGCTCTGTA TGCTGATGGA GAGGGCTCGT CTGTGTCTGA TAATGGCAAA
    ACACTGCCCC GCAGTGGCCC CAGGAACCTG CGGGTTTATG ATCCCACCAC CAGCACGCTG
    TCGGTGGGCT GGGAACATGC AGAAGGGCCT GTGATTCAGT ACCGCATTGG CTACGCACCC
    ACCACTGGAG ATCCCATTGA AGAATTTACA ACTGTCCCAG GCAACCGTAA CTCGGTCATC
    CTGCAAAACC TGCAGCCAGA CACGCCGTAC AACATCAACG TAGAAGCCAT TTATCAAGAT
    GGCCCTGGAG GACGACTCAG TGGAGATGGA AAAACATTGG ATCTCCTTGG GCCTCGTAAC
    CTGCGTGTTT CAGACGAGTG GTACACACGT TTCCGAGTGT CCTGGGATCC TGCAGCCGCA
    CGGGTGAATG GATATAAACT GGTCTATACA CCAGTGGGCA CAACCGACAC CATGGAAGCC
    TTCGTTGGAG ACGTGACTTC ACACCACCTG AACAACCTCC AGCCTGGCAC CACATACGAC
    CTGAAGGTTT ACGCTCAGTA TGATGCGGGC TTGAGCGGCG CTCTGGTCGG ACAGGGAACC
    ACCCTGTATC TCAACGTGAC CGACCTCACT TCCTACAACG TGGGCTACGA CTCTTTCTGC
    ATCAGATGGA CTCCTCATCG AGCTGCCACC TCCTACAGAC TCAAAGTCAA CCCTTTCAAC
    CCATCTCACC GTGGAGCTCA GGAGGTCACG ATCACTGCGG CTGAGAGCCA GTACTGTTTT
    GACGGCCTGA CGCCAGACGC GCTATACAAC GCCACCGTCT ACACGCAGAC CCCCAACCGA
    GAGGGGCCCG GAGTCAGTGT CAAAGAGCGC ACACTGGTGA AGCCCACCGT CGCCCCAACA
    CTACCTCCAA CTCCTCCACC TCCAGCCACC ATTCCTTCTG CACTCGAGGT GTGCAAAGGT
    GCCAAGGCTG ACCTGGTGTT CCTGATCGAT GGCTCGTGGA GTATCGGAGA TGACAGCTTC
    GCTAAAGTCC GGCAGTTTGT GTTCAGCATG ATTGGGGCGT TTGATGTGGT CAGCCATGAG
    GGCATGCAGG TGTCTTTCGT GCAGTACAGC GACGACGCCA AAACTGAGTT CAAGCTCAAC
    ACCTACCACG ACAAGGGAAT GGTGCTGTCT GCTCTTCAGA TGGTCAATTA TAGAGGAGGA
    AACACCAAAA CAGGTGCTGC ACTGAAGCAT GTTGGAGAAA AAGTGTTCAC CTCGGACAAT
    GGCATGAGGA GAACCGTTCC CAAGGTACTG GTTGTGGTGA CCGATGGACG TTCTCAAGAT
    GACGTGAAGA AGAGCGCTGC TAAACTACAG CATGCAGGTT ACAGTGTGTT TGTGGTGGGT
    GTCGCTGACA TTGACGTTAA TGAACTGAGG AACATTGGAA GCAAGCCCAG TGACAGACAC
    GTCTTTATCG TCGACAACTT CGATGCTTTC GCCAAGATCC AAGACAACCT GATCACGTTC
    ATCTGTGAAA CGGCAACGTC CACATGCCCT TTGATTTACC TCAATGGATT CACATCACCA
    GGCTTCAGGA TGCTGGAAGC TTTCAACATC ACTGACAAGA CCTACGCAGG CATGAACGGC
    GTCTCTCTGG AAACCGGCTC CTTCAACAGC TTCATCGCAT ACAGACTCCA TAAGGACGCC
    TTCATTTCCC AGCCAACAAA GGAAATCCAT CCAGATGGTC TTCCTCCATC CTACACCCTC
    ATCCTCCTCT TCCGTCTGCT CCCGGACACC GGCAAGGATG CGTTTGATTT ATGGCAGATC
    TCAGATAAGA ACAATAACCC TGAGGTCGGG CTCACCATTG ACCCCTCCAG CAGAGTGATC
    TCCTTCTACA ATAAAGACAC TAGAGGGGAA ATTCAGAAAG TCTCCTTTGA TAACGACCAC
    GTGAAGAAGA TTTTCCACGG CAGTTTCCAC AAGCTTCACA TTTCTATTTC GCCGAAAAAT
    GTCAAACTCA ACATCGACTG CCAAGAGGTG GCTGAGAAAC AGATCAAAGC AGCCAACAAC
    GTCTCCACAG ATGGCTACGA AGTCCTTGGA AAAATGACAA AGTCTGGCGG TTCAAAGAAG
    CAGTCGGCCA CATTCCAGCT CCAGATGTTT GATATTGTCT GCAGTTTGGG CTGGACAAGT
    CGAGACAAAT GTTGTGATAT TCCAGCAATG CGAGATGAAG CCAAGTGTCC TGCTCTTCCT
    CACTCCTGCA CATGTGCTCA GGACAGTGTT GGTCCTCCAG GCCCAGCGGG TCCTCAAGGC
    AGTCCTGGTA CAAAAGGCCC ACGAGGCGAG AGAGGAGAGT CTGGTCCTAC GGGTCCAATG
    GGTCCACGTG GAGAGCAGGG TCTGCCAGGG CAGATGGGGC CACCGGGTCC ACAGGGCCCC
    AATGGACTAT CCCTTCCCGG AGAACCAGGG CGACCGGGAC CTAAGGGAGA CCCCGGTGAC
    TCAGGTTTAC CTGGACAGAG AGGCCCGGTT GGCGCTGCAG GTCCACTCGG CCCTGTTGGA
    CCCGCTGGAG CAAGAGGTCC ACCAGGAAGT GAAGGCCCAT CTGGACCAAG AGGTCCATCG
    GGACCAATGG GACCACCGGG AGCTCCCGGA ATGCCAGGAA TCACCGGAAA ACCAGGAAAA
    CCCGGAGACA CTGGCTTTCC TGGACCTGTT GGCATGAAAG GAGAGAAGGG TGAAAGAGGA
    GACACTGCGT CACAGAATAT GATGCGCTCT GTAGCGAGAC AAGTCTGCGA ACAGCTAGTC
    AGCAGACAAA TGAGCAGAAT TGATATGATG ATGAACCAGA TCCCCAGCAA CTACAGGAGT
    AACAGCCCCG GCCCCGCTGG ACCTCCAGGC CCACCTGGAA ACCAGGGACC CCATGGAGAG
    CCTGGTCAGC CCGGACCCAA TGGCTTCCCA GGAAATCCAG GTTTACCAGG CTCTCCTGGA
    GAAAGAGGAG TGCCTGGAGA GAAGGGTGAA CGAGGCAGCC CCGGCATTGG CACTCGAGGA
    CAGAGAGGAT TACCTGGACC TCCTGGGCCA CCAGGGCAGT CTCAGACCGG TCCCCCTGGT
    CCCTCAGGCT CACCCGGGCC CCGAGGACCA CCAGGACAAC ATGGTTCTTC TGGAGTCACA
    GGACCACCAG GACCTCCAGG ATACTGTGAC TCCTCTCAGT GCGGCATGCC TTACAACGGA
    CAAGGCTACC CAGGCCCCTA CCAGCCCGGG TCTGAAACCT ACGTAGTGCC CATACCAGCC
    GCGCCATCGG AAGATATCGA GACACAGTCG CCAGGATTGC GCTACCAGCG CGGAAAAAGG
    TCGCTATCAA GAAAGGAAGC CGAAAGAATC AAAACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009291571.1
    CDS140..9478
    Translation

    Target ORF information:

    RefSeq Version XM_009293296.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio collagen, type XII, alpha 1a (col12a1a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009293296.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    ATGAAGATCA GGTTGTCTTT GGCCGCTGCT GCGCTCTTGA CTCTAGTACT CAGTTCAGTA 
    GAGGCTCAAG TGGAGCCTCC GTCAGACTTA AAATTTAAAA TCCTGAATGA AAACACAGTG
    CAAATGTCAT GGAGGAAGCC CTCGTCTCAG ATACAGGGCT ACAGAATACA GGTCACGTCA
    GATACAGATG ATTCTAAAGA ATTGAGTCTT CCTGCCTCGT CCACAAGCAC ATCAATTACA
    GATCTAACTC CAGATGTAGA CTATTCAGTG TCAATCAATT CATATGATGG TGCTGAAGAA
    AGTATCCCTA TCCTTGGACA AATCACAATT CAATCCAGCA ACACAAGTGG ACCAAGGAGG
    CCACAGTCAG AGGCTGTCAA GTGTTCAGTG AGTGCCGTTG CGGATCTGGT TTTCCTGGTG
    GACGGCTCTT GGAGTGTGGG AAGAGAAAAC TTTAGATTTA TCCGGAGTTT CATTGGGGCC
    ATGGCCGGGG CCTTCGACAT TGAAGAGGAT AAGACCAGAG TGGCTGTGGT TCAGTACAGC
    TCGGACACCA GGACAGAGTT CAGTCTAAAC ACACATTTCA GACGACCCGA TGTCCTGCGA
    GCCATTAAAA ATCTGCCGTA CAAGGGTGGA AATACCATGA CGGGTGATGC AATGGACTAT
    CTGGTGAAAA ACACGTTCAC TCAGGCGGCT GGAGCCAGGA AAGGCTTCCC AAAAGTGGCC
    ATGATCATCA CAGATGGAAA ATCTCAAGAC CCCGTAGAGG AGTACGCCGA ACGGTTGAGG
    AACATCGGAG TGGAGATCTT TGTGCTGGGT ATCAAAGGAG CAGATGAGGA CGAGCTGAAG
    GAGATCGCCT CCAGACCCCA TAGCAAGCAT GTCTATAACG TCCCCAACTT CGACATGATC
    AATCAGGTCC AGAAGGCGCT CATCACTGAA GTTTGCTCTG GCGTGGAGGA GCAGCTAAAT
    TCCCTTGTAA GCGGGGAGGA AGTTGTCGAA CCAGCTTCCA ACCTCAGAGT GACAGATATC
    TCCTCCAAAT CCATGAGAAT TGCGTGGGAT TCATCTCCTG GTGATATCAC AGGCTATAAG
    CTGCAGGTTT TTCCCACTAT TGCGAGCGCC AAAAAGCAGG AGCTCTACAC TGGGCCCACG
    ATGACCTTGG CCAATGTCAG AGACCTGACG CCTGAGATGG AGTATGAGAT CAGTCTGTTT
    GCTCTCAAAG GTCTGACTCC TAGTGTGCCG CAAGTTGTCA CTGAGAAAAC TCAGCCAGTG
    AAAGTATTTA CAGAGTGTTC CCTAGAGGTG GATGTCCAAG CTGACATTGT CCTCCTGGTG
    GATGGGTCAT ATAGTATAGG ACTGCCTAAC TTTGCGAAGG TTAGAGCTTT CCTCGAAGTA
    CTTGTGAATT CTTTTGACAT CGGACCTAAC AAAGTGCAGA TCAGCTTGGT TCAGTACAGC
    AGGGATCCTC ACACTGAGTT CGCCTTGAAT AAATTCGATG ACAATGCAGC CATGGTCAAG
    GCGGTCCGCA CCTTCCCCTA CCGAGGTGGA TCCACCAACA CCGGCAAGGC CATGACATAT
    GTGAGGGAAA AGATCTTTGT TTCGGGCCGT GGCGCTCGTG ATAACGTCCC CCGAGTCATG
    GTGCTTATCA CAGACGGCAA GTCCTCAGAT TCTTTCAAAG ACCCCGCCAA CAAACTGAGG
    GATACAGATG TGGAGATTTT TGCAGTGGGA GTAAAAGATG CCGTACGATC TGAGCTGGAG
    GCTATCGCCA ATGTTCCTGC TGATAACCAT GTGTTTGAGG TGGAGGACTT TGATGCTTTC
    GAGCGGATCT CCAAGGAGCT GACCGCGTCC ATCTGCCTCA GAATTGAGCA GGAGCTCCAT
    AACATCAGGA AAAGAAGTTT GCTTCCCCCA AAGTCACTGA GCTTCTCTGA GGTGACCTCC
    AGGAGTTTCA GGACCTCATG GGTTTCTGAT GGAGAGGATA TCCTGTCTTT TCTGGTGCGC
    TTCAGGCCAG CGGCGGACCT AACTGGAGAC TACATCTCTC TGGTGGTTCC CCATGACATC
    ACCAGTACAA TTCTGCCCAA TCTCACGCCA CTCACCACCT ACGAAGTTAA CGTCATTGCT
    CAGTATGATA AAGGAGACAG CTTTCCTTTG ACGGGCGAGG AGACCACTTT AGAAGAGTTG
    GGTTCTGTTC GTAACCTGAT GGTGAGCGAG GAGGCTGTGG ACCGCTTTCG GGTGTCGTGG
    AGAGCTGCTC CAGGATTAGT GCTGAGGTAC AGACTGCTCT ACGAGCCTGT GAGCGGAGGG
    GATAAACTGG AAGCAGAGAC TGATGGGAAT CAGGTCACCA CTGTGCTCAA TGAACTACTA
    CCGGTCACTA CTTACCGGGT CACCGTTTAC CCTGAGTACG CAAGTGGAAG GGGTCCCTCC
    ATGGACACTG AAGGCACCAC CAAAGAAGTT CGAGGTTCTC CACGCGACTT AAGGGTCTTC
    AATGAGACGG TGTCTAGCAT GAGGGTCTCG TGGCGGGCTG CCCCAGGCAA CGTGCTTCAA
    TATCAGGTGG CCTACAAACC TGACGGTGGG GAGAGGAAAG AAATAAGTGT TAAAGGAGAG
    ACAACAACTG CTGTGCTGAA AAATCTGCTG CCGGACACGG AGTATGAGCT GTTTGTTAGC
    GCACGTTACG TCTCTGGACT TGGCGCACCA CTTATGGGAA CAGGCACCAC GCTGGAAGAA
    CTTGGTTCAC CTTCGAATCT GGTAACTTCA GACGTCACTG AGAACAGTTT TGTAGCATCC
    TGGACCGCCG CCCCTGGAAA CGTCCGTCTT TATCGGGTCA CCTGGAAATC CCTCTTTTCA
    GAGGAAGCTG GCGAGAAGAC TGTCCCCGGG GACGTAACCA CCACTGTTCT GGACGGGCTG
    ACACCAGAGA CCCGCTACAA GGTTTCTGTC TACGCTGTCT ACAGCCGTGC GGAGGGGGCG
    CCACTGCACG GAGAGGAGAC CACAGATGTG TCGGCCGCTA CCAGAGCTCT TACGGTGTCA
    GAAGAAACAG AGAGGACCAT GAAGGTGACA TGGATACCAG CACCTGGCAA TGTTGTCAAT
    TACAGGGTAT CATACAAACC TCAGGGTGAT TTACGCCAAA TGGCCTCCAA AGCACCAGGA
    GGCACCAATT CTATAGTCCT GAGACGCCTG ATCCCCCAGA CCACTTACGA TATCACAGTG
    GTTCCCGTCT ACAAGTTTGG GGATGGAAGA CAAAGGCAAG GAGAAGGAAC TACATTGTCT
    CAATATAAAG CTCCCAGAAA CTTGCAAACT TCGGAGCCCA CTAAAACCAG TTTCAGGGTG
    ACCTGGGATC ATGCTCCTGG AGATGTAAAA GGCTACAAGG TCACTTTCCA TCCCACCGGA
    AAGGAAGTGG ACCTGGGAGA GTTACTCGTA GGCCCCTATG ACAACACTGT GGTTCTGGAG
    GAACTAAGGG CCGGCACTAA GTATACGGTA AATGTGTTTG GTATGTTTGA TGGAGGAGAG
    AGTATGCCAT TGGCTGGAGA GGAGAAGACC ACCCTCTCTG ACGCAGCTTC TGAAAACCTC
    CCTTATACAC CTTCAGATGC CCAGTGTAAA ACCACGGCCC AAGCGGATAT CGTCCTGCTT
    GTTGATGGAT CTTGGAGCAT TGGTCGCCTG AACTTCAAGA CCATACGCAA TTTCATTGCT
    CGCATGGTGG GAGTCTTCGA CATCGGCCCC GATCGTGTCC AGATTGGTCT GGCACAGTAC
    AGCGGAGACC CGAAGACTGA GTGGCATCTC AATGCTCACA GAACCAGGAC TCAGCTGCTG
    GATGCGGTGG CCAATCTACC CTACAAAGGA GGAAACACTC TCACCGGCCT TGCTTTGAAT
    TACATCCTTC AGAATAACTT CAAGCCTAAC GTTGGCATGC GTCCAAACTC CAGAAAGATC
    GGCGTCCTGG TCACTGATGG CAAGTCCCAA GACGACATTG TGGCGAACTC ACAGAACCTG
    CGGGACCAAG GGATTGAACT GTACGCCATT GGTGTAAAGA ACGCAGATGA GAATGAGTTG
    AGAACCATTG CTTCCGATCC CGATGACATT CACATGTACA ACGTGGCTGA TTTCTCCTTC
    CTGCTGGACA TTGTGGATGA CCTCACAAAC AACCTGTGCA ACAGCGTCAA AGGTCCAGGA
    GGAGGACCAG AGCCACCCAC TGATCTGACC ACCTCAGAGG TCACACATTA CTCTTTCCGT
    GCCACTTGGG TGGCTCCTGA CGGGCCTGTG GAGAAGTACA GAGTGGAGTA TGTGAGCACC
    GCTGGAGGAG AACCACAGCA ACAGATGTTT GTGGACGGGA CGGTGACCAC TGTGGTTTTG
    GAAGGTCTCA CTCCGCTGAC CGAGTACATA GTAAAGGTTT ACTCAGTGGT GGGAGAGGAC
    AGCAGCGACC CACTCAGAGG CATAGAGACC ACATTGCCTC TCAGTGCTGT GAGGAGCATG
    AACATCTACA AAGAGCGCTC CACCACCATG AATGTGAGAT GGGACGCAGC CACAGGGGCC
    ACAGGCTACA TGCTTCTCTA CAAGGCCCTT AACGCCACTG AACCCTCTGT GGAGCAGGAG
    ATGCGAGTGA AAGGAATGGT CACTGACGTC CAGCTGAAGA AACTTCTCCC CAACACGGCC
    TACAGTGTTT CGCTCTTCGC CCTGCACGGC GATTACGCCA GTGAACCTCT GATTGATCAA
    GGGGTGACCC TGCCTCTTCC TCCATCCGGT GAGTTGAGGA TTACCGATGT GACCCACAGC
    ACCATGCAGC TGGACTGGGA CGCTGCGCCC GGCGCCGTCC GAAAGTACAT CATCACTTAC
    AAGCCAGAGG AGGGAGAGGC GAAGGAGCTT GAAGTTGACG GAGATGTAAC ATCTAGAGGC
    CTGGATAATC TCATCTCCCA GACTGAATAT GATGTGGCTG TGACTCCGGT TTATGATTCG
    GGCACTGGAA ACCCCATGCT TAACCAGGCC ATTACAGATG TGGTTCCTGC CCCGCAGAAC
    TTGAGGTTCT CGATGGTGAC CCAGACCAGT TTTAGGGTTC ACTGGGAGCA CGGCGCTCCT
    GATGTCTCCC TCTACCGTAT TGGCTGGAAA AAGAAAGGAA CGACCGAGTT TCAAAATGAT
    ATCCTGAGTA GTGAAGACAC TACATATGAT CTGGTGGACC TGGAGCCAGA CACACCTTAT
    GATGTTTCGG TCACTGCCAT TTACCCTGAT GAGTCTGAGA GCGAGGATCT GCTGGGCACT
    GAACGCACTT TGGCCAAGCT TGCTACACCA CCTCCCAGTG GTCCTCCTCA AAACCTCCAG
    GTGTTCGACG AGACCACCAC CTCCCTCACG ACTAAGTGGG ACCACGCGCC TGGACGAGTT
    CAGAACTACA AAATCACCTA CGTACCGCTT ACTGGAGGAC GATCACAGTC GACGCAGGTC
    GGAGGAAAGA AAAACACAGT GATCCTGCAG AAACTGACTC CAGACACTCC TTACTCCATC
    ACTGTTGCTG CGGTCTACGC CAGCGGAGAG GCCAGAGACA TTTCTGGCCA AGGAAAGACA
    TTGCCGCTGG GCAGTGTCAG GAATCTTCAG GTCACTGACC CCACAGTTAG CACTCTGAAT
    GTGCGCTGGG AGCCGGCGGA GGGAAGCGTT CGCCAGTACA GGATCTACTA CCAGCCCGTC
    GCAGGGGGCG CAGAGGACAT GGTCCAGGTT CCTGGAAGCA CCACCAGCAC CGTTCTGAAG
    AACCTGCAAT CTGACACTCA GTACAGAGTG ACGGTGGTGC CAGTCTACGC TCCTGGAGAG
    GGCAAGCGCA TGTCGGAGAC CGGAAAAACA CTGGTTCGGA CCCCTGCCAA GAACATCCAG
    GTTTACAACC CCACCACAAA CACCCTGAAC GTGCGCTGGG AGCCGGCCTC AGGACAGGTG
    CAGCAGTACC GCGTGGCTTA CGCCCCTCTC TCCGGGATCC GACCCTCTCA GTCGGTGCTG
    GTTCCAGGAA ACACCCACAC GGCTCTGCTG CAGCAGCTGG AATCAGACAC TGACTACTCT
    GTGAGTGTGG TGGCTCTGTA TGCTGATGGA GAGGGCTCGT CTGTGTCTGA TAATGGCAAA
    ACACTGCCCC GCAGTGGCCC CAGGAACCTG CGGGTTTATG ATCCCACCAC CAGCACGCTG
    TCGGTGGGCT GGGAACATGC AGAAGGGCCT GTGATTCAGT ACCGCATTGG CTACGCACCC
    ACCACTGGAG ATCCCATTGA AGAATTTACA ACTGTCCCAG GCAACCGTAA CTCGGTCATC
    CTGCAAAACC TGCAGCCAGA CACGCCGTAC AACATCAACG TAGAAGCCAT TTATCAAGAT
    GGCCCTGGAG GACGACTCAG TGGAGATGGA AAAACATTGG ATCTCCTTGG GCCTCGTAAC
    CTGCGTGTTT CAGACGAGTG GTACACACGT TTCCGAGTGT CCTGGGATCC TGCAGCCGCA
    CGGGTGAATG GATATAAACT GGTCTATACA CCAGTGGGCA CAACCGACAC CATGGAAGCC
    TTCGTTGGAG ACGTGACTTC ACACCACCTG AACAACCTCC AGCCTGGCAC CACATACGAC
    CTGAAGGTTT ACGCTCAGTA TGATGCGGGC TTGAGCGGCG CTCTGGTCGG ACAGGGAACC
    ACCCTGTATC TCAACGTGAC CGACCTCACT TCCTACAACG TGGGCTACGA CTCTTTCTGC
    ATCAGATGGA CTCCTCATCG AGCTGCCACC TCCTACAGAC TCAAAGTCAA CCCTTTCAAC
    CCATCTCACC GTGGAGCTCA GGAGGTCACG ATCACTGCGG CTGAGAGCCA GTACTGTTTT
    GACGGCCTGA CGCCAGACGC GCTATACAAC GCCACCGTCT ACACGCAGAC CCCCAACCGA
    GAGGGGCCCG GAGTCAGTGT CAAAGAGCGC ACACTGGTGA AGCCCACCGT CGCCCCAACA
    CTACCTCCAA CTCCTCCACC TCCAGCCACC ATTCCTTCTG CACTCGAGGT GTGCAAAGGT
    GCCAAGGCTG ACCTGGTGTT CCTGATCGAT GGCTCGTGGA GTATCGGAGA TGACAGCTTC
    GCTAAAGTCC GGCAGTTTGT GTTCAGCATG ATTGGGGCGT TTGATGTGGT CAGCCATGAG
    GGCATGCAGG TGTCTTTCGT GCAGTACAGC GACGACGCCA AAACTGAGTT CAAGCTCAAC
    ACCTACCACG ACAAGGGAAT GGTGCTGTCT GCTCTTCAGA TGGTCAATTA TAGAGGAGGA
    AACACCAAAA CAGGTGCTGC ACTGAAGCAT GTTGGAGAAA AAGTGTTCAC CTCGGACAAT
    GGCATGAGGA GAACCGTTCC CAAGGTACTG GTTGTGGTGA CCGATGGACG TTCTCAAGAT
    GACGTGAAGA AGAGCGCTGC TAAACTACAG CATGCAGGTT ACAGTGTGTT TGTGGTGGGT
    GTCGCTGACA TTGACGTTAA TGAACTGAGG AACATTGGAA GCAAGCCCAG TGACAGACAC
    GTCTTTATCG TCGACAACTT CGATGCTTTC GCCAAGATCC AAGACAACCT GATCACGTTC
    ATCTGTGAAA CGGCAACGTC CACATGCCCT TTGATTTACC TCAATGGATT CACATCACCA
    GGCTTCAGGA TGCTGGAAGC TTTCAACATC ACTGACAAGA CCTACGCAGG CATGAACGGC
    GTCTCTCTGG AAACCGGCTC CTTCAACAGC TTCATCGCAT ACAGACTCCA TAAGGACGCC
    TTCATTTCCC AGCCAACAAA GGAAATCCAT CCAGATGGTC TTCCTCCATC CTACACCCTC
    ATCCTCCTCT TCCGTCTGCT CCCGGACACC GGCAAGGATG CGTTTGATTT ATGGCAGATC
    TCAGATAAGA ACAATAACCC TGAGGTCGGG CTCACCATTG ACCCCTCCAG CAGAGTGATC
    TCCTTCTACA ATAAAGACAC TAGAGGGGAA ATTCAGAAAG TCTCCTTTGA TAACGACCAC
    GTGAAGAAGA TTTTCCACGG CAGTTTCCAC AAGCTTCACA TTTCTATTTC GCCGAAAAAT
    GTCAAACTCA ACATCGACTG CCAAGAGGTG GCTGAGAAAC AGATCAAAGC AGCCAACAAC
    GTCTCCACAG ATGGCTACGA AGTCCTTGGA AAAATGACAA AGTCTGGCGG TTCAAAGAAG
    CAGTCGGCCA CATTCCAGCT CCAGATGTTT GATATTGTCT GCAGTTTGGG CTGGACAAGT
    CGAGACAAAT GTTGTGATAT TCCAGCAATG CGAGATGAAG CCAAGTGTCC TGCTCTTCCT
    CACTCCTGCA CATGTGCTCA GGACAGTGTT GGTCCTCCAG GCCCAGCGGG TCCTCAAGGC
    AGTCCTGGTA CAAAAGGCCC ACGAGGCGAG AGAGGAGAGT CTGGTCCTAC GGGTCCAATG
    GGTCCACGTG GAGAGCAGGG TCTGCCAGGG CAGATGGGGC CACCGGGTCC ACAGGGCCCC
    AATGGACTAT CCCTTCCCGG AGAACCAGGG CGACCGGGAC CTAAGGGAGA CCCCGGTGAC
    TCAGGTTTAC CTGGACAGAG AGGCCCGGTT GGCGCTGCAG GTCCACTCGG CCCTGTTGGA
    CCCGCTGGAG CAAGAGGTCC ACCAGGAAGT GAAGGCCCAT CTGGACCAAG AGGTCCATCG
    GGACCAATGG GACCACCGGG AGCTCCCGGA ATGCCAGGAA TCACCGGAAA ACCAGGAAAA
    CCCGGAGACA CTGGCTTTCC TGGACCTGTT GGCATGAAAG GAGAGAAGGG TGAAAGAGGA
    GACACTGCGT CACAGAATAT GATGCGCTCT GTAGCGAGAC AAGTCTGCGA ACAGCTAGTC
    AGCAGACAAA TGAGCAGAAT TGATATGATG ATGAACCAGA TCCCCAGCAA CTACAGGAGT
    AACAGCCCCG GCCCCGCTGG ACCTCCAGGC CCACCTGGAA ACCAGGGACC CCATGGAGAG
    CCTGGTCAGC CCGGACCCAA TGGCTTCCCA GGAAATCCAG GTTTACCAGG CTCTCCTGGA
    GAAAGAGGAG TGCCTGGAGA GAAGGGTGAA CGAGGCAGCC CCGGCATTGG CACTCGAGGA
    CAGAGAGGAT TACCTGGACC TCCTGGGCCA CCAGGGCAGT CTCAGACCGG TCCCCCTGGT
    CCCTCAGGCT CACCCGGGCC CCGAGGACCA CCAGGACAAC ATGGTTCTTC TGGAGTCACA
    GGACCACCAG GACCTCCAGG ATACTGTGAC TCCTCTCAGT GCGGCATGCC TTACAACGGA
    CAAGGCTACC CAGGCCCCTA CCAGCCCGGG TCTGAAACCT ACGTAGTGCC CATACCAGCC
    GCGCCATCGG AAGATATCGA GACACAGTCG CCAGGATTGC GCTACCAGCG CGGAAAAAGG
    TCGCTATCAA GAAAGGAAGC CGAAAGAATC AAAACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48772
    Clone ID Related Accession (Same CDS sequence) XM_009293296.2 , XM_009293296.3
    Accession Version XM_009293296.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9339bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product collagen alpha-1(XII) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293296.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    ATGAAGATCA GGTTGTCTTT GGCCGCTGCT GCGCTCTTGA CTCTAGTACT CAGTTCAGTA 
    GAGGCTCAAG TGGAGCCTCC GTCAGACTTA AAATTTAAAA TCCTGAATGA AAACACAGTG
    CAAATGTCAT GGAGGAAGCC CTCGTCTCAG ATACAGGGCT ACAGAATACA GGTCACGTCA
    GATACAGATG ATTCTAAAGA ATTGAGTCTT CCTGCCTCGT CCACAAGCAC ATCAATTACA
    GATCTAACTC CAGATGTAGA CTATTCAGTG TCAATCAATT CATATGATGG TGCTGAAGAA
    AGTATCCCTA TCCTTGGACA AATCACAATT CAATCCAGCA ACACAAGTGG ACCAAGGAGG
    CCACAGTCAG AGGCTGTCAA GTGTTCAGTG AGTGCCGTTG CGGATCTGGT TTTCCTGGTG
    GACGGCTCTT GGAGTGTGGG AAGAGAAAAC TTTAGATTTA TCCGGAGTTT CATTGGGGCC
    ATGGCCGGGG CCTTCGACAT TGAAGAGGAT AAGACCAGAG TGGCTGTGGT TCAGTACAGC
    TCGGACACCA GGACAGAGTT CAGTCTAAAC ACACATTTCA GACGACCCGA TGTCCTGCGA
    GCCATTAAAA ATCTGCCGTA CAAGGGTGGA AATACCATGA CGGGTGATGC AATGGACTAT
    CTGGTGAAAA ACACGTTCAC TCAGGCGGCT GGAGCCAGGA AAGGCTTCCC AAAAGTGGCC
    ATGATCATCA CAGATGGAAA ATCTCAAGAC CCCGTAGAGG AGTACGCCGA ACGGTTGAGG
    AACATCGGAG TGGAGATCTT TGTGCTGGGT ATCAAAGGAG CAGATGAGGA CGAGCTGAAG
    GAGATCGCCT CCAGACCCCA TAGCAAGCAT GTCTATAACG TCCCCAACTT CGACATGATC
    AATCAGGTCC AGAAGGCGCT CATCACTGAA GTTTGCTCTG GCGTGGAGGA GCAGCTAAAT
    TCCCTTGTAA GCGGGGAGGA AGTTGTCGAA CCAGCTTCCA ACCTCAGAGT GACAGATATC
    TCCTCCAAAT CCATGAGAAT TGCGTGGGAT TCATCTCCTG GTGATATCAC AGGCTATAAG
    CTGCAGGTTT TTCCCACTAT TGCGAGCGCC AAAAAGCAGG AGCTCTACAC TGGGCCCACG
    ATGACCTTGG CCAATGTCAG AGACCTGACG CCTGAGATGG AGTATGAGAT CAGTCTGTTT
    GCTCTCAAAG GTCTGACTCC TAGTGTGCCG CAAGTTGTCA CTGAGAAAAC TCAGCCAGTG
    AAAGTATTTA CAGAGTGTTC CCTAGAGGTG GATGTCCAAG CTGACATTGT CCTCCTGGTG
    GATGGGTCAT ATAGTATAGG ACTGCCTAAC TTTGCGAAGG TTAGAGCTTT CCTCGAAGTA
    CTTGTGAATT CTTTTGACAT CGGACCTAAC AAAGTGCAGA TCAGCTTGGT TCAGTACAGC
    AGGGATCCTC ACACTGAGTT CGCCTTGAAT AAATTCGATG ACAATGCAGC CATGGTCAAG
    GCGGTCCGCA CCTTCCCCTA CCGAGGTGGA TCCACCAACA CCGGCAAGGC CATGACATAT
    GTGAGGGAAA AGATCTTTGT TTCGGGCCGT GGCGCTCGTG ATAACGTCCC CCGAGTCATG
    GTGCTTATCA CAGACGGCAA GTCCTCAGAT TCTTTCAAAG ACCCCGCCAA CAAACTGAGG
    GATACAGATG TGGAGATTTT TGCAGTGGGA GTAAAAGATG CCGTACGATC TGAGCTGGAG
    GCTATCGCCA ATGTTCCTGC TGATAACCAT GTGTTTGAGG TGGAGGACTT TGATGCTTTC
    GAGCGGATCT CCAAGGAGCT GACCGCGTCC ATCTGCCTCA GAATTGAGCA GGAGCTCCAT
    AACATCAGGA AAAGAAGTTT GCTTCCCCCA AAGTCACTGA GCTTCTCTGA GGTGACCTCC
    AGGAGTTTCA GGACCTCATG GGTTTCTGAT GGAGAGGATA TCCTGTCTTT TCTGGTGCGC
    TTCAGGCCAG CGGCGGACCT AACTGGAGAC TACATCTCTC TGGTGGTTCC CCATGACATC
    ACCAGTACAA TTCTGCCCAA TCTCACGCCA CTCACCACCT ACGAAGTTAA CGTCATTGCT
    CAGTATGATA AAGGAGACAG CTTTCCTTTG ACGGGCGAGG AGACCACTTT AGAAGAGTTG
    GGTTCTGTTC GTAACCTGAT GGTGAGCGAG GAGGCTGTGG ACCGCTTTCG GGTGTCGTGG
    AGAGCTGCTC CAGGATTAGT GCTGAGGTAC AGACTGCTCT ACGAGCCTGT GAGCGGAGGG
    GATAAACTGG AAGCAGAGAC TGATGGGAAT CAGGTCACCA CTGTGCTCAA TGAACTACTA
    CCGGTCACTA CTTACCGGGT CACCGTTTAC CCTGAGTACG CAAGTGGAAG GGGTCCCTCC
    ATGGACACTG AAGGCACCAC CAAAGAAGTT CGAGGTTCTC CACGCGACTT AAGGGTCTTC
    AATGAGACGG TGTCTAGCAT GAGGGTCTCG TGGCGGGCTG CCCCAGGCAA CGTGCTTCAA
    TATCAGGTGG CCTACAAACC TGACGGTGGG GAGAGGAAAG AAATAAGTGT TAAAGGAGAG
    ACAACAACTG CTGTGCTGAA AAATCTGCTG CCGGACACGG AGTATGAGCT GTTTGTTAGC
    GCACGTTACG TCTCTGGACT TGGCGCACCA CTTATGGGAA CAGGCACCAC GCTGGAAGAA
    CTTGGTTCAC CTTCGAATCT GGTAACTTCA GACGTCACTG AGAACAGTTT TGTAGCATCC
    TGGACCGCCG CCCCTGGAAA CGTCCGTCTT TATCGGGTCA CCTGGAAATC CCTCTTTTCA
    GAGGAAGCTG GCGAGAAGAC TGTCCCCGGG GACGTAACCA CCACTGTTCT GGACGGGCTG
    ACACCAGAGA CCCGCTACAA GGTTTCTGTC TACGCTGTCT ACAGCCGTGC GGAGGGGGCG
    CCACTGCACG GAGAGGAGAC CACAGATGTG TCGGCCGCTA CCAGAGCTCT TACGGTGTCA
    GAAGAAACAG AGAGGACCAT GAAGGTGACA TGGATACCAG CACCTGGCAA TGTTGTCAAT
    TACAGGGTAT CATACAAACC TCAGGGTGAT TTACGCCAAA TGGCCTCCAA AGCACCAGGA
    GGCACCAATT CTATAGTCCT GAGACGCCTG ATCCCCCAGA CCACTTACGA TATCACAGTG
    GTTCCCGTCT ACAAGTTTGG GGATGGAAGA CAAAGGCAAG GAGAAGGAAC TACATTGTCT
    CAATATAAAG CTCCCAGAAA CTTGCAAACT TCGGAGCCCA CTAAAACCAG TTTCAGGGTG
    ACCTGGGATC ATGCTCCTGG AGATGTAAAA GGCTACAAGG TCACTTTCCA TCCCACCGGA
    AAGGAAGTGG ACCTGGGAGA GTTACTCGTA GGCCCCTATG ACAACACTGT GGTTCTGGAG
    GAACTAAGGG CCGGCACTAA GTATACGGTA AATGTGTTTG GTATGTTTGA TGGAGGAGAG
    AGTATGCCAT TGGCTGGAGA GGAGAAGACC ACCCTCTCTG ACGCAGCTTC TGAAAACCTC
    CCTTATACAC CTTCAGATGC CCAGTGTAAA ACCACGGCCC AAGCGGATAT CGTCCTGCTT
    GTTGATGGAT CTTGGAGCAT TGGTCGCCTG AACTTCAAGA CCATACGCAA TTTCATTGCT
    CGCATGGTGG GAGTCTTCGA CATCGGCCCC GATCGTGTCC AGATTGGTCT GGCACAGTAC
    AGCGGAGACC CGAAGACTGA GTGGCATCTC AATGCTCACA GAACCAGGAC TCAGCTGCTG
    GATGCGGTGG CCAATCTACC CTACAAAGGA GGAAACACTC TCACCGGCCT TGCTTTGAAT
    TACATCCTTC AGAATAACTT CAAGCCTAAC GTTGGCATGC GTCCAAACTC CAGAAAGATC
    GGCGTCCTGG TCACTGATGG CAAGTCCCAA GACGACATTG TGGCGAACTC ACAGAACCTG
    CGGGACCAAG GGATTGAACT GTACGCCATT GGTGTAAAGA ACGCAGATGA GAATGAGTTG
    AGAACCATTG CTTCCGATCC CGATGACATT CACATGTACA ACGTGGCTGA TTTCTCCTTC
    CTGCTGGACA TTGTGGATGA CCTCACAAAC AACCTGTGCA ACAGCGTCAA AGGTCCAGGA
    GGAGGACCAG AGCCACCCAC TGATCTGACC ACCTCAGAGG TCACACATTA CTCTTTCCGT
    GCCACTTGGG TGGCTCCTGA CGGGCCTGTG GAGAAGTACA GAGTGGAGTA TGTGAGCACC
    GCTGGAGGAG AACCACAGCA ACAGATGTTT GTGGACGGGA CGGTGACCAC TGTGGTTTTG
    GAAGGTCTCA CTCCGCTGAC CGAGTACATA GTAAAGGTTT ACTCAGTGGT GGGAGAGGAC
    AGCAGCGACC CACTCAGAGG CATAGAGACC ACATTGCCTC TCAGTGCTGT GAGGAGCATG
    AACATCTACA AAGAGCGCTC CACCACCATG AATGTGAGAT GGGACGCAGC CACAGGGGCC
    ACAGGCTACA TGCTTCTCTA CAAGGCCCTT AACGCCACTG AACCCTCTGT GGAGCAGGAG
    ATGCGAGTGA AAGGAATGGT CACTGACGTC CAGCTGAAGA AACTTCTCCC CAACACGGCC
    TACAGTGTTT CGCTCTTCGC CCTGCACGGC GATTACGCCA GTGAACCTCT GATTGATCAA
    GGGGTGACCC TGCCTCTTCC TCCATCCGGT GAGTTGAGGA TTACCGATGT GACCCACAGC
    ACCATGCAGC TGGACTGGGA CGCTGCGCCC GGCGCCGTCC GAAAGTACAT CATCACTTAC
    AAGCCAGAGG AGGGAGAGGC GAAGGAGCTT GAAGTTGACG GAGATGTAAC ATCTAGAGGC
    CTGGATAATC TCATCTCCCA GACTGAATAT GATGTGGCTG TGACTCCGGT TTATGATTCG
    GGCACTGGAA ACCCCATGCT TAACCAGGCC ATTACAGATG TGGTTCCTGC CCCGCAGAAC
    TTGAGGTTCT CGATGGTGAC CCAGACCAGT TTTAGGGTTC ACTGGGAGCA CGGCGCTCCT
    GATGTCTCCC TCTACCGTAT TGGCTGGAAA AAGAAAGGAA CGACCGAGTT TCAAAATGAT
    ATCCTGAGTA GTGAAGACAC TACATATGAT CTGGTGGACC TGGAGCCAGA CACACCTTAT
    GATGTTTCGG TCACTGCCAT TTACCCTGAT GAGTCTGAGA GCGAGGATCT GCTGGGCACT
    GAACGCACTT TGGCCAAGCT TGCTACACCA CCTCCCAGTG GTCCTCCTCA AAACCTCCAG
    GTGTTCGACG AGACCACCAC CTCCCTCACG ACTAAGTGGG ACCACGCGCC TGGACGAGTT
    CAGAACTACA AAATCACCTA CGTACCGCTT ACTGGAGGAC GATCACAGTC GACGCAGGTC
    GGAGGAAAGA AAAACACAGT GATCCTGCAG AAACTGACTC CAGACACTCC TTACTCCATC
    ACTGTTGCTG CGGTCTACGC CAGCGGAGAG GCCAGAGACA TTTCTGGCCA AGGAAAGACA
    TTGCCGCTGG GCAGTGTCAG GAATCTTCAG GTCACTGACC CCACAGTTAG CACTCTGAAT
    GTGCGCTGGG AGCCGGCGGA GGGAAGCGTT CGCCAGTACA GGATCTACTA CCAGCCCGTC
    GCAGGGGGCG CAGAGGACAT GGTCCAGGTT CCTGGAAGCA CCACCAGCAC CGTTCTGAAG
    AACCTGCAAT CTGACACTCA GTACAGAGTG ACGGTGGTGC CAGTCTACGC TCCTGGAGAG
    GGCAAGCGCA TGTCGGAGAC CGGAAAAACA CTGGTTCGGA CCCCTGCCAA GAACATCCAG
    GTTTACAACC CCACCACAAA CACCCTGAAC GTGCGCTGGG AGCCGGCCTC AGGACAGGTG
    CAGCAGTACC GCGTGGCTTA CGCCCCTCTC TCCGGGATCC GACCCTCTCA GTCGGTGCTG
    GTTCCAGGAA ACACCCACAC GGCTCTGCTG CAGCAGCTGG AATCAGACAC TGACTACTCT
    GTGAGTGTGG TGGCTCTGTA TGCTGATGGA GAGGGCTCGT CTGTGTCTGA TAATGGCAAA
    ACACTGCCCC GCAGTGGCCC CAGGAACCTG CGGGTTTATG ATCCCACCAC CAGCACGCTG
    TCGGTGGGCT GGGAACATGC AGAAGGGCCT GTGATTCAGT ACCGCATTGG CTACGCACCC
    ACCACTGGAG ATCCCATTGA AGAATTTACA ACTGTCCCAG GCAACCGTAA CTCGGTCATC
    CTGCAAAACC TGCAGCCAGA CACGCCGTAC AACATCAACG TAGAAGCCAT TTATCAAGAT
    GGCCCTGGAG GACGACTCAG TGGAGATGGA AAAACATTGG ATCTCCTTGG GCCTCGTAAC
    CTGCGTGTTT CAGACGAGTG GTACACACGT TTCCGAGTGT CCTGGGATCC TGCAGCCGCA
    CGGGTGAATG GATATAAACT GGTCTATACA CCAGTGGGCA CAACCGACAC CATGGAAGCC
    TTCGTTGGAG ACGTGACTTC ACACCACCTG AACAACCTCC AGCCTGGCAC CACATACGAC
    CTGAAGGTTT ACGCTCAGTA TGATGCGGGC TTGAGCGGCG CTCTGGTCGG ACAGGGAACC
    ACCCTGTATC TCAACGTGAC CGACCTCACT TCCTACAACG TGGGCTACGA CTCTTTCTGC
    ATCAGATGGA CTCCTCATCG AGCTGCCACC TCCTACAGAC TCAAAGTCAA CCCTTTCAAC
    CCATCTCACC GTGGAGCTCA GGAGGTCACG ATCACTGCGG CTGAGAGCCA GTACTGTTTT
    GACGGCCTGA CGCCAGACGC GCTATACAAC GCCACCGTCT ACACGCAGAC CCCCAACCGA
    GAGGGGCCCG GAGTCAGTGT CAAAGAGCGC ACACTGGTGA AGCCCACCGT CGCCCCAACA
    CTACCTCCAA CTCCTCCACC TCCAGCCACC ATTCCTTCTG CACTCGAGGT GTGCAAAGGT
    GCCAAGGCTG ACCTGGTGTT CCTGATCGAT GGCTCGTGGA GTATCGGAGA TGACAGCTTC
    GCTAAAGTCC GGCAGTTTGT GTTCAGCATG ATTGGGGCGT TTGATGTGGT CAGCCATGAG
    GGCATGCAGG TGTCTTTCGT GCAGTACAGC GACGACGCCA AAACTGAGTT CAAGCTCAAC
    ACCTACCACG ACAAGGGAAT GGTGCTGTCT GCTCTTCAGA TGGTCAATTA TAGAGGAGGA
    AACACCAAAA CAGGTGCTGC ACTGAAGCAT GTTGGAGAAA AAGTGTTCAC CTCGGACAAT
    GGCATGAGGA GAACCGTTCC CAAGGTACTG GTTGTGGTGA CCGATGGACG TTCTCAAGAT
    GACGTGAAGA AGAGCGCTGC TAAACTACAG CATGCAGGTT ACAGTGTGTT TGTGGTGGGT
    GTCGCTGACA TTGACGTTAA TGAACTGAGG AACATTGGAA GCAAGCCCAG TGACAGACAC
    GTCTTTATCG TCGACAACTT CGATGCTTTC GCCAAGATCC AAGACAACCT GATCACGTTC
    ATCTGTGAAA CGGCAACGTC CACATGCCCT TTGATTTACC TCAATGGATT CACATCACCA
    GGCTTCAGGA TGCTGGAAGC TTTCAACATC ACTGACAAGA CCTACGCAGG CATGAACGGC
    GTCTCTCTGG AAACCGGCTC CTTCAACAGC TTCATCGCAT ACAGACTCCA TAAGGACGCC
    TTCATTTCCC AGCCAACAAA GGAAATCCAT CCAGATGGTC TTCCTCCATC CTACACCCTC
    ATCCTCCTCT TCCGTCTGCT CCCGGACACC GGCAAGGATG CGTTTGATTT ATGGCAGATC
    TCAGATAAGA ACAATAACCC TGAGGTCGGG CTCACCATTG ACCCCTCCAG CAGAGTGATC
    TCCTTCTACA ATAAAGACAC TAGAGGGGAA ATTCAGAAAG TCTCCTTTGA TAACGACCAC
    GTGAAGAAGA TTTTCCACGG CAGTTTCCAC AAGCTTCACA TTTCTATTTC GCCGAAAAAT
    GTCAAACTCA ACATCGACTG CCAAGAGGTG GCTGAGAAAC AGATCAAAGC AGCCAACAAC
    GTCTCCACAG ATGGCTACGA AGTCCTTGGA AAAATGACAA AGTCTGGCGG TTCAAAGAAG
    CAGTCGGCCA CATTCCAGCT CCAGATGTTT GATATTGTCT GCAGTTTGGG CTGGACAAGT
    CGAGACAAAT GTTGTGATAT TCCAGCAATG CGAGATGAAG CCAAGTGTCC TGCTCTTCCT
    CACTCCTGCA CATGTGCTCA GGACAGTGTT GGTCCTCCAG GCCCAGCGGG TCCTCAAGGC
    AGTCCTGGTA CAAAAGGCCC ACGAGGCGAG AGAGGAGAGT CTGGTCCTAC GGGTCCAATG
    GGTCCACGTG GAGAGCAGGG TCTGCCAGGG CAGATGGGGC CACCGGGTCC ACAGGGCCCC
    AATGGACTAT CCCTTCCCGG AGAACCAGGG CGACCGGGAC CTAAGGGAGA CCCCGGTGAC
    TCAGGTTTAC CTGGACAGAG AGGCCCGGTT GGCGCTGCAG GTCCACTCGG CCCTGTTGGA
    CCCGCTGGAG CAAGAGGTCC ACCAGGAAGT GAAGGCCCAT CTGGACCAAG AGGTCCATCG
    GGACCAATGG GACCACCGGG AGCTCCCGGA ATGCCAGGAA TCACCGGAAA ACCAGGAAAA
    CCCGGAGACA CTGGCTTTCC TGGACCTGTT GGCATGAAAG GAGAGAAGGG TGAAAGAGGA
    GACACTGCGT CACAGAATAT GATGCGCTCT GTAGCGAGAC AAGTCTGCGA ACAGCTAGTC
    AGCAGACAAA TGAGCAGAAT TGATATGATG ATGAACCAGA TCCCCAGCAA CTACAGGAGT
    AACAGCCCCG GCCCCGCTGG ACCTCCAGGC CCACCTGGAA ACCAGGGACC CCATGGAGAG
    CCTGGTCAGC CCGGACCCAA TGGCTTCCCA GGAAATCCAG GTTTACCAGG CTCTCCTGGA
    GAAAGAGGAG TGCCTGGAGA GAAGGGTGAA CGAGGCAGCC CCGGCATTGG CACTCGAGGA
    CAGAGAGGAT TACCTGGACC TCCTGGGCCA CCAGGGCAGT CTCAGACCGG TCCCCCTGGT
    CCCTCAGGCT CACCCGGGCC CCGAGGACCA CCAGGACAAC ATGGTTCTTC TGGAGTCACA
    GGACCACCAG GACCTCCAGG ATACTGTGAC TCCTCTCAGT GCGGCATGCC TTACAACGGA
    CAAGGCTACC CAGGCCCCTA CCAGCCCGGG TCTGAAACCT ACGTAGTGCC CATACCAGCC
    GCGCCATCGG AAGATATCGA GACACAGTCG CCAGGATTGC GCTACCAGCG CGGAAAAAGG
    TCGCTATCAA GAAAGGAAGC CGAAAGAATC AAAACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009291571.1
    CDS180..9518
    Translation

    Target ORF information:

    RefSeq Version XM_009293296.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio collagen, type XII, alpha 1a (col12a1a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009293296.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    ATGAAGATCA GGTTGTCTTT GGCCGCTGCT GCGCTCTTGA CTCTAGTACT CAGTTCAGTA 
    GAGGCTCAAG TGGAGCCTCC GTCAGACTTA AAATTTAAAA TCCTGAATGA AAACACAGTG
    CAAATGTCAT GGAGGAAGCC CTCGTCTCAG ATACAGGGCT ACAGAATACA GGTCACGTCA
    GATACAGATG ATTCTAAAGA ATTGAGTCTT CCTGCCTCGT CCACAAGCAC ATCAATTACA
    GATCTAACTC CAGATGTAGA CTATTCAGTG TCAATCAATT CATATGATGG TGCTGAAGAA
    AGTATCCCTA TCCTTGGACA AATCACAATT CAATCCAGCA ACACAAGTGG ACCAAGGAGG
    CCACAGTCAG AGGCTGTCAA GTGTTCAGTG AGTGCCGTTG CGGATCTGGT TTTCCTGGTG
    GACGGCTCTT GGAGTGTGGG AAGAGAAAAC TTTAGATTTA TCCGGAGTTT CATTGGGGCC
    ATGGCCGGGG CCTTCGACAT TGAAGAGGAT AAGACCAGAG TGGCTGTGGT TCAGTACAGC
    TCGGACACCA GGACAGAGTT CAGTCTAAAC ACACATTTCA GACGACCCGA TGTCCTGCGA
    GCCATTAAAA ATCTGCCGTA CAAGGGTGGA AATACCATGA CGGGTGATGC AATGGACTAT
    CTGGTGAAAA ACACGTTCAC TCAGGCGGCT GGAGCCAGGA AAGGCTTCCC AAAAGTGGCC
    ATGATCATCA CAGATGGAAA ATCTCAAGAC CCCGTAGAGG AGTACGCCGA ACGGTTGAGG
    AACATCGGAG TGGAGATCTT TGTGCTGGGT ATCAAAGGAG CAGATGAGGA CGAGCTGAAG
    GAGATCGCCT CCAGACCCCA TAGCAAGCAT GTCTATAACG TCCCCAACTT CGACATGATC
    AATCAGGTCC AGAAGGCGCT CATCACTGAA GTTTGCTCTG GCGTGGAGGA GCAGCTAAAT
    TCCCTTGTAA GCGGGGAGGA AGTTGTCGAA CCAGCTTCCA ACCTCAGAGT GACAGATATC
    TCCTCCAAAT CCATGAGAAT TGCGTGGGAT TCATCTCCTG GTGATATCAC AGGCTATAAG
    CTGCAGGTTT TTCCCACTAT TGCGAGCGCC AAAAAGCAGG AGCTCTACAC TGGGCCCACG
    ATGACCTTGG CCAATGTCAG AGACCTGACG CCTGAGATGG AGTATGAGAT CAGTCTGTTT
    GCTCTCAAAG GTCTGACTCC TAGTGTGCCG CAAGTTGTCA CTGAGAAAAC TCAGCCAGTG
    AAAGTATTTA CAGAGTGTTC CCTAGAGGTG GATGTCCAAG CTGACATTGT CCTCCTGGTG
    GATGGGTCAT ATAGTATAGG ACTGCCTAAC TTTGCGAAGG TTAGAGCTTT CCTCGAAGTA
    CTTGTGAATT CTTTTGACAT CGGACCTAAC AAAGTGCAGA TCAGCTTGGT TCAGTACAGC
    AGGGATCCTC ACACTGAGTT CGCCTTGAAT AAATTCGATG ACAATGCAGC CATGGTCAAG
    GCGGTCCGCA CCTTCCCCTA CCGAGGTGGA TCCACCAACA CCGGCAAGGC CATGACATAT
    GTGAGGGAAA AGATCTTTGT TTCGGGCCGT GGCGCTCGTG ATAACGTCCC CCGAGTCATG
    GTGCTTATCA CAGACGGCAA GTCCTCAGAT TCTTTCAAAG ACCCCGCCAA CAAACTGAGG
    GATACAGATG TGGAGATTTT TGCAGTGGGA GTAAAAGATG CCGTACGATC TGAGCTGGAG
    GCTATCGCCA ATGTTCCTGC TGATAACCAT GTGTTTGAGG TGGAGGACTT TGATGCTTTC
    GAGCGGATCT CCAAGGAGCT GACCGCGTCC ATCTGCCTCA GAATTGAGCA GGAGCTCCAT
    AACATCAGGA AAAGAAGTTT GCTTCCCCCA AAGTCACTGA GCTTCTCTGA GGTGACCTCC
    AGGAGTTTCA GGACCTCATG GGTTTCTGAT GGAGAGGATA TCCTGTCTTT TCTGGTGCGC
    TTCAGGCCAG CGGCGGACCT AACTGGAGAC TACATCTCTC TGGTGGTTCC CCATGACATC
    ACCAGTACAA TTCTGCCCAA TCTCACGCCA CTCACCACCT ACGAAGTTAA CGTCATTGCT
    CAGTATGATA AAGGAGACAG CTTTCCTTTG ACGGGCGAGG AGACCACTTT AGAAGAGTTG
    GGTTCTGTTC GTAACCTGAT GGTGAGCGAG GAGGCTGTGG ACCGCTTTCG GGTGTCGTGG
    AGAGCTGCTC CAGGATTAGT GCTGAGGTAC AGACTGCTCT ACGAGCCTGT GAGCGGAGGG
    GATAAACTGG AAGCAGAGAC TGATGGGAAT CAGGTCACCA CTGTGCTCAA TGAACTACTA
    CCGGTCACTA CTTACCGGGT CACCGTTTAC CCTGAGTACG CAAGTGGAAG GGGTCCCTCC
    ATGGACACTG AAGGCACCAC CAAAGAAGTT CGAGGTTCTC CACGCGACTT AAGGGTCTTC
    AATGAGACGG TGTCTAGCAT GAGGGTCTCG TGGCGGGCTG CCCCAGGCAA CGTGCTTCAA
    TATCAGGTGG CCTACAAACC TGACGGTGGG GAGAGGAAAG AAATAAGTGT TAAAGGAGAG
    ACAACAACTG CTGTGCTGAA AAATCTGCTG CCGGACACGG AGTATGAGCT GTTTGTTAGC
    GCACGTTACG TCTCTGGACT TGGCGCACCA CTTATGGGAA CAGGCACCAC GCTGGAAGAA
    CTTGGTTCAC CTTCGAATCT GGTAACTTCA GACGTCACTG AGAACAGTTT TGTAGCATCC
    TGGACCGCCG CCCCTGGAAA CGTCCGTCTT TATCGGGTCA CCTGGAAATC CCTCTTTTCA
    GAGGAAGCTG GCGAGAAGAC TGTCCCCGGG GACGTAACCA CCACTGTTCT GGACGGGCTG
    ACACCAGAGA CCCGCTACAA GGTTTCTGTC TACGCTGTCT ACAGCCGTGC GGAGGGGGCG
    CCACTGCACG GAGAGGAGAC CACAGATGTG TCGGCCGCTA CCAGAGCTCT TACGGTGTCA
    GAAGAAACAG AGAGGACCAT GAAGGTGACA TGGATACCAG CACCTGGCAA TGTTGTCAAT
    TACAGGGTAT CATACAAACC TCAGGGTGAT TTACGCCAAA TGGCCTCCAA AGCACCAGGA
    GGCACCAATT CTATAGTCCT GAGACGCCTG ATCCCCCAGA CCACTTACGA TATCACAGTG
    GTTCCCGTCT ACAAGTTTGG GGATGGAAGA CAAAGGCAAG GAGAAGGAAC TACATTGTCT
    CAATATAAAG CTCCCAGAAA CTTGCAAACT TCGGAGCCCA CTAAAACCAG TTTCAGGGTG
    ACCTGGGATC ATGCTCCTGG AGATGTAAAA GGCTACAAGG TCACTTTCCA TCCCACCGGA
    AAGGAAGTGG ACCTGGGAGA GTTACTCGTA GGCCCCTATG ACAACACTGT GGTTCTGGAG
    GAACTAAGGG CCGGCACTAA GTATACGGTA AATGTGTTTG GTATGTTTGA TGGAGGAGAG
    AGTATGCCAT TGGCTGGAGA GGAGAAGACC ACCCTCTCTG ACGCAGCTTC TGAAAACCTC
    CCTTATACAC CTTCAGATGC CCAGTGTAAA ACCACGGCCC AAGCGGATAT CGTCCTGCTT
    GTTGATGGAT CTTGGAGCAT TGGTCGCCTG AACTTCAAGA CCATACGCAA TTTCATTGCT
    CGCATGGTGG GAGTCTTCGA CATCGGCCCC GATCGTGTCC AGATTGGTCT GGCACAGTAC
    AGCGGAGACC CGAAGACTGA GTGGCATCTC AATGCTCACA GAACCAGGAC TCAGCTGCTG
    GATGCGGTGG CCAATCTACC CTACAAAGGA GGAAACACTC TCACCGGCCT TGCTTTGAAT
    TACATCCTTC AGAATAACTT CAAGCCTAAC GTTGGCATGC GTCCAAACTC CAGAAAGATC
    GGCGTCCTGG TCACTGATGG CAAGTCCCAA GACGACATTG TGGCGAACTC ACAGAACCTG
    CGGGACCAAG GGATTGAACT GTACGCCATT GGTGTAAAGA ACGCAGATGA GAATGAGTTG
    AGAACCATTG CTTCCGATCC CGATGACATT CACATGTACA ACGTGGCTGA TTTCTCCTTC
    CTGCTGGACA TTGTGGATGA CCTCACAAAC AACCTGTGCA ACAGCGTCAA AGGTCCAGGA
    GGAGGACCAG AGCCACCCAC TGATCTGACC ACCTCAGAGG TCACACATTA CTCTTTCCGT
    GCCACTTGGG TGGCTCCTGA CGGGCCTGTG GAGAAGTACA GAGTGGAGTA TGTGAGCACC
    GCTGGAGGAG AACCACAGCA ACAGATGTTT GTGGACGGGA CGGTGACCAC TGTGGTTTTG
    GAAGGTCTCA CTCCGCTGAC CGAGTACATA GTAAAGGTTT ACTCAGTGGT GGGAGAGGAC
    AGCAGCGACC CACTCAGAGG CATAGAGACC ACATTGCCTC TCAGTGCTGT GAGGAGCATG
    AACATCTACA AAGAGCGCTC CACCACCATG AATGTGAGAT GGGACGCAGC CACAGGGGCC
    ACAGGCTACA TGCTTCTCTA CAAGGCCCTT AACGCCACTG AACCCTCTGT GGAGCAGGAG
    ATGCGAGTGA AAGGAATGGT CACTGACGTC CAGCTGAAGA AACTTCTCCC CAACACGGCC
    TACAGTGTTT CGCTCTTCGC CCTGCACGGC GATTACGCCA GTGAACCTCT GATTGATCAA
    GGGGTGACCC TGCCTCTTCC TCCATCCGGT GAGTTGAGGA TTACCGATGT GACCCACAGC
    ACCATGCAGC TGGACTGGGA CGCTGCGCCC GGCGCCGTCC GAAAGTACAT CATCACTTAC
    AAGCCAGAGG AGGGAGAGGC GAAGGAGCTT GAAGTTGACG GAGATGTAAC ATCTAGAGGC
    CTGGATAATC TCATCTCCCA GACTGAATAT GATGTGGCTG TGACTCCGGT TTATGATTCG
    GGCACTGGAA ACCCCATGCT TAACCAGGCC ATTACAGATG TGGTTCCTGC CCCGCAGAAC
    TTGAGGTTCT CGATGGTGAC CCAGACCAGT TTTAGGGTTC ACTGGGAGCA CGGCGCTCCT
    GATGTCTCCC TCTACCGTAT TGGCTGGAAA AAGAAAGGAA CGACCGAGTT TCAAAATGAT
    ATCCTGAGTA GTGAAGACAC TACATATGAT CTGGTGGACC TGGAGCCAGA CACACCTTAT
    GATGTTTCGG TCACTGCCAT TTACCCTGAT GAGTCTGAGA GCGAGGATCT GCTGGGCACT
    GAACGCACTT TGGCCAAGCT TGCTACACCA CCTCCCAGTG GTCCTCCTCA AAACCTCCAG
    GTGTTCGACG AGACCACCAC CTCCCTCACG ACTAAGTGGG ACCACGCGCC TGGACGAGTT
    CAGAACTACA AAATCACCTA CGTACCGCTT ACTGGAGGAC GATCACAGTC GACGCAGGTC
    GGAGGAAAGA AAAACACAGT GATCCTGCAG AAACTGACTC CAGACACTCC TTACTCCATC
    ACTGTTGCTG CGGTCTACGC CAGCGGAGAG GCCAGAGACA TTTCTGGCCA AGGAAAGACA
    TTGCCGCTGG GCAGTGTCAG GAATCTTCAG GTCACTGACC CCACAGTTAG CACTCTGAAT
    GTGCGCTGGG AGCCGGCGGA GGGAAGCGTT CGCCAGTACA GGATCTACTA CCAGCCCGTC
    GCAGGGGGCG CAGAGGACAT GGTCCAGGTT CCTGGAAGCA CCACCAGCAC CGTTCTGAAG
    AACCTGCAAT CTGACACTCA GTACAGAGTG ACGGTGGTGC CAGTCTACGC TCCTGGAGAG
    GGCAAGCGCA TGTCGGAGAC CGGAAAAACA CTGGTTCGGA CCCCTGCCAA GAACATCCAG
    GTTTACAACC CCACCACAAA CACCCTGAAC GTGCGCTGGG AGCCGGCCTC AGGACAGGTG
    CAGCAGTACC GCGTGGCTTA CGCCCCTCTC TCCGGGATCC GACCCTCTCA GTCGGTGCTG
    GTTCCAGGAA ACACCCACAC GGCTCTGCTG CAGCAGCTGG AATCAGACAC TGACTACTCT
    GTGAGTGTGG TGGCTCTGTA TGCTGATGGA GAGGGCTCGT CTGTGTCTGA TAATGGCAAA
    ACACTGCCCC GCAGTGGCCC CAGGAACCTG CGGGTTTATG ATCCCACCAC CAGCACGCTG
    TCGGTGGGCT GGGAACATGC AGAAGGGCCT GTGATTCAGT ACCGCATTGG CTACGCACCC
    ACCACTGGAG ATCCCATTGA AGAATTTACA ACTGTCCCAG GCAACCGTAA CTCGGTCATC
    CTGCAAAACC TGCAGCCAGA CACGCCGTAC AACATCAACG TAGAAGCCAT TTATCAAGAT
    GGCCCTGGAG GACGACTCAG TGGAGATGGA AAAACATTGG ATCTCCTTGG GCCTCGTAAC
    CTGCGTGTTT CAGACGAGTG GTACACACGT TTCCGAGTGT CCTGGGATCC TGCAGCCGCA
    CGGGTGAATG GATATAAACT GGTCTATACA CCAGTGGGCA CAACCGACAC CATGGAAGCC
    TTCGTTGGAG ACGTGACTTC ACACCACCTG AACAACCTCC AGCCTGGCAC CACATACGAC
    CTGAAGGTTT ACGCTCAGTA TGATGCGGGC TTGAGCGGCG CTCTGGTCGG ACAGGGAACC
    ACCCTGTATC TCAACGTGAC CGACCTCACT TCCTACAACG TGGGCTACGA CTCTTTCTGC
    ATCAGATGGA CTCCTCATCG AGCTGCCACC TCCTACAGAC TCAAAGTCAA CCCTTTCAAC
    CCATCTCACC GTGGAGCTCA GGAGGTCACG ATCACTGCGG CTGAGAGCCA GTACTGTTTT
    GACGGCCTGA CGCCAGACGC GCTATACAAC GCCACCGTCT ACACGCAGAC CCCCAACCGA
    GAGGGGCCCG GAGTCAGTGT CAAAGAGCGC ACACTGGTGA AGCCCACCGT CGCCCCAACA
    CTACCTCCAA CTCCTCCACC TCCAGCCACC ATTCCTTCTG CACTCGAGGT GTGCAAAGGT
    GCCAAGGCTG ACCTGGTGTT CCTGATCGAT GGCTCGTGGA GTATCGGAGA TGACAGCTTC
    GCTAAAGTCC GGCAGTTTGT GTTCAGCATG ATTGGGGCGT TTGATGTGGT CAGCCATGAG
    GGCATGCAGG TGTCTTTCGT GCAGTACAGC GACGACGCCA AAACTGAGTT CAAGCTCAAC
    ACCTACCACG ACAAGGGAAT GGTGCTGTCT GCTCTTCAGA TGGTCAATTA TAGAGGAGGA
    AACACCAAAA CAGGTGCTGC ACTGAAGCAT GTTGGAGAAA AAGTGTTCAC CTCGGACAAT
    GGCATGAGGA GAACCGTTCC CAAGGTACTG GTTGTGGTGA CCGATGGACG TTCTCAAGAT
    GACGTGAAGA AGAGCGCTGC TAAACTACAG CATGCAGGTT ACAGTGTGTT TGTGGTGGGT
    GTCGCTGACA TTGACGTTAA TGAACTGAGG AACATTGGAA GCAAGCCCAG TGACAGACAC
    GTCTTTATCG TCGACAACTT CGATGCTTTC GCCAAGATCC AAGACAACCT GATCACGTTC
    ATCTGTGAAA CGGCAACGTC CACATGCCCT TTGATTTACC TCAATGGATT CACATCACCA
    GGCTTCAGGA TGCTGGAAGC TTTCAACATC ACTGACAAGA CCTACGCAGG CATGAACGGC
    GTCTCTCTGG AAACCGGCTC CTTCAACAGC TTCATCGCAT ACAGACTCCA TAAGGACGCC
    TTCATTTCCC AGCCAACAAA GGAAATCCAT CCAGATGGTC TTCCTCCATC CTACACCCTC
    ATCCTCCTCT TCCGTCTGCT CCCGGACACC GGCAAGGATG CGTTTGATTT ATGGCAGATC
    TCAGATAAGA ACAATAACCC TGAGGTCGGG CTCACCATTG ACCCCTCCAG CAGAGTGATC
    TCCTTCTACA ATAAAGACAC TAGAGGGGAA ATTCAGAAAG TCTCCTTTGA TAACGACCAC
    GTGAAGAAGA TTTTCCACGG CAGTTTCCAC AAGCTTCACA TTTCTATTTC GCCGAAAAAT
    GTCAAACTCA ACATCGACTG CCAAGAGGTG GCTGAGAAAC AGATCAAAGC AGCCAACAAC
    GTCTCCACAG ATGGCTACGA AGTCCTTGGA AAAATGACAA AGTCTGGCGG TTCAAAGAAG
    CAGTCGGCCA CATTCCAGCT CCAGATGTTT GATATTGTCT GCAGTTTGGG CTGGACAAGT
    CGAGACAAAT GTTGTGATAT TCCAGCAATG CGAGATGAAG CCAAGTGTCC TGCTCTTCCT
    CACTCCTGCA CATGTGCTCA GGACAGTGTT GGTCCTCCAG GCCCAGCGGG TCCTCAAGGC
    AGTCCTGGTA CAAAAGGCCC ACGAGGCGAG AGAGGAGAGT CTGGTCCTAC GGGTCCAATG
    GGTCCACGTG GAGAGCAGGG TCTGCCAGGG CAGATGGGGC CACCGGGTCC ACAGGGCCCC
    AATGGACTAT CCCTTCCCGG AGAACCAGGG CGACCGGGAC CTAAGGGAGA CCCCGGTGAC
    TCAGGTTTAC CTGGACAGAG AGGCCCGGTT GGCGCTGCAG GTCCACTCGG CCCTGTTGGA
    CCCGCTGGAG CAAGAGGTCC ACCAGGAAGT GAAGGCCCAT CTGGACCAAG AGGTCCATCG
    GGACCAATGG GACCACCGGG AGCTCCCGGA ATGCCAGGAA TCACCGGAAA ACCAGGAAAA
    CCCGGAGACA CTGGCTTTCC TGGACCTGTT GGCATGAAAG GAGAGAAGGG TGAAAGAGGA
    GACACTGCGT CACAGAATAT GATGCGCTCT GTAGCGAGAC AAGTCTGCGA ACAGCTAGTC
    AGCAGACAAA TGAGCAGAAT TGATATGATG ATGAACCAGA TCCCCAGCAA CTACAGGAGT
    AACAGCCCCG GCCCCGCTGG ACCTCCAGGC CCACCTGGAA ACCAGGGACC CCATGGAGAG
    CCTGGTCAGC CCGGACCCAA TGGCTTCCCA GGAAATCCAG GTTTACCAGG CTCTCCTGGA
    GAAAGAGGAG TGCCTGGAGA GAAGGGTGAA CGAGGCAGCC CCGGCATTGG CACTCGAGGA
    CAGAGAGGAT TACCTGGACC TCCTGGGCCA CCAGGGCAGT CTCAGACCGG TCCCCCTGGT
    CCCTCAGGCT CACCCGGGCC CCGAGGACCA CCAGGACAAC ATGGTTCTTC TGGAGTCACA
    GGACCACCAG GACCTCCAGG ATACTGTGAC TCCTCTCAGT GCGGCATGCC TTACAACGGA
    CAAGGCTACC CAGGCCCCTA CCAGCCCGGG TCTGAAACCT ACGTAGTGCC CATACCAGCC
    GCGCCATCGG AAGATATCGA GACACAGTCG CCAGGATTGC GCTACCAGCG CGGAAAAAGG
    TCGCTATCAA GAAAGGAAGC CGAAAGAATC AAAACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48773
    Clone ID Related Accession (Same CDS sequence) XM_009293297.3 , XM_009293297.2
    Accession Version XM_009293297.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9063bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product collagen alpha-1(XII) chain isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293297.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    ATGAAGATCA GGTTGTCTTT GGCCGCTGCT GCGCTCTTGA CTCTAGTACT CAGTTCAGTA 
    GAGGCTCAAG TGGAGCCTCC GTCAGACTTA AAATTTAAAA TCCTGAATGA AAACACAGTG
    CAAATGTCAT GGAGGAAGCC CTCGTCTCAG ATACAGGGCT ACAGAATACA GGTCACGTCA
    GATACAGATG ATTCTAAAGA ATTGAGTCTT CCTGCCTCGT CCACAAGCAC ATCAATTACA
    GATCTAACTC CAGATGTAGA CTATTCAGTG TCAATCAATT CATATGATGG TGCTGAAGAA
    AGTATCCCTA TCCTTGGACA AATCACAATT CAATCCAGCA ACACAAGTGG ACCAAGGAGG
    CCACAGTCAG AGGCTGTCAA GTGTTCAGTG AGTGCCGTTG CGGATCTGGT TTTCCTGGTG
    GACGGCTCTT GGAGTGTGGG AAGAGAAAAC TTTAGATTTA TCCGGAGTTT CATTGGGGCC
    ATGGCCGGGG CCTTCGACAT TGAAGAGGAT AAGACCAGAG TGGCTGTGGT TCAGTACAGC
    TCGGACACCA GGACAGAGTT CAGTCTAAAC ACACATTTCA GACGACCCGA TGTCCTGCGA
    GCCATTAAAA ATCTGCCGTA CAAGGGTGGA AATACCATGA CGGGTGATGC AATGGACTAT
    CTGGTGAAAA ACACGTTCAC TCAGGCGGCT GGAGCCAGGA AAGGCTTCCC AAAAGTGGCC
    ATGATCATCA CAGATGGAAA ATCTCAAGAC CCCGTAGAGG AGTACGCCGA ACGGTTGAGG
    AACATCGGAG TGGAGATCTT TGTGCTGGGT ATCAAAGGAG CAGATGAGGA CGAGCTGAAG
    GAGATCGCCT CCAGACCCCA TAGCAAGCAT GTCTATAACG TCCCCAACTT CGACATGATC
    AATCAGGTCC AGAAGGCGCT CATCACTGAA GTTTGCTCTG GCGTGGAGGA GCAGCTAAAT
    TCCCTTGTAA GCGGGGAGGA AGTTGTCGAA CCAGCTTCCA ACCTCAGAGT GACAGATATC
    TCCTCCAAAT CCATGAGAAT TGCGTGGGAT TCATCTCCTG GTGATATCAC AGGCTATAAG
    CTGCAGGTTT TTCCCACTAT TGCGAGCGCC AAAAAGCAGG AGCTCTACAC TGGGCCCACG
    ATGACCTTGG CCAATGTCAG AGACCTGACG CCTGAGATGG AGTATGAGAT CAGTCTGTTT
    GCTCTCAAAG GTCTGACTCC TAGTGTGCCG CAAGTTGTCA CTGAGAAAAC TCAGCCAGTG
    AAAGTATTTA CAGAGTGTTC CCTAGAGGTG GATGTCCAAG CTGACATTGT CCTCCTGGTG
    GATGGGTCAT ATAGTATAGG ACTGCCTAAC TTTGCGAAGG TTAGAGCTTT CCTCGAAGTA
    CTTGTGAATT CTTTTGACAT CGGACCTAAC AAAGTGCAGA TCAGCTTGGT TCAGTACAGC
    AGGGATCCTC ACACTGAGTT CGCCTTGAAT AAATTCGATG ACAATGCAGC CATGGTCAAG
    GCGGTCCGCA CCTTCCCCTA CCGAGGTGGA TCCACCAACA CCGGCAAGGC CATGACATAT
    GTGAGGGAAA AGATCTTTGT TTCGGGCCGT GGCGCTCGTG ATAACGTCCC CCGAGTCATG
    GTGCTTATCA CAGACGGCAA GTCCTCAGAT TCTTTCAAAG ACCCCGCCAA CAAACTGAGG
    GATACAGATG TGGAGATTTT TGCAGTGGGA GTAAAAGATG CCGTACGATC TGAGCTGGAG
    GCTATCGCCA ATGTTCCTGC TGATAACCAT GTGTTTGAGG TGGAGGACTT TGATGCTTTC
    GAGCGGATCT CCAAGGAGCT GACCGCGTCC ATCTGCCTCA GAATTGAGCA GGAGCTCCAT
    AACATCAGGA AAAGAAGTTT GCTTCCCCCA AAGTCACTGA GCTTCTCTGA GGTGACCTCC
    AGGAGTTTCA GGACCTCATG GGTTTCTGAT GGAGAGGATA TCCTGTCTTT TCTGGTGCGC
    TTCAGGCCAG CGGCGGACCT AACTGGAGAC TACATCTCTC TGGTGGTTCC CCATGACATC
    ACCAGTACAA TTCTGCCCAA TCTCACGCCA CTCACCACCT ACGAAGTTAA CGTCATTGCT
    CAGTATGATA AAGGAGACAG CTTTCCTTTG ACGGGCGAGG AGACCACTTT AGAAGAGTTG
    GGTTCTGTTC GTAACCTGAT GGTGAGCGAG GAGGCTGTGG ACCGCTTTCG GGTGTCGTGG
    AGAGCTGCTC CAGGATTAGT GCTGAGGTAC AGACTGCTCT ACGAGCCTGT GAGCGGAGGG
    GATAAACTGG AAGCAGAGAC TGATGGGAAT CAGGTCACCA CTGTGCTCAA TGAACTACTA
    CCGGTCACTA CTTACCGGGT CACCGTTTAC CCTGAGTACG CAAGTGGAAG GGGTCCCTCC
    ATGGACACTG AAGGCACCAC CAAAGAAGTT CGAGGTTCTC CACGCGACTT AAGGGTCTTC
    AATGAGACGG TGTCTAGCAT GAGGGTCTCG TGGCGGGCTG CCCCAGGCAA CGTGCTTCAA
    TATCAGGTGG CCTACAAACC TGACGGTGGG GAGAGGAAAG AAATAAGTGT TAAAGGAGAG
    ACAACAACTG CTGTGCTGAA AAATCTGCTG CCGGACACGG AGTATGAGCT GTTTGTTAGC
    GCACGTTACG TCTCTGGACT TGGCGCACCA CTTATGGGAA CAGGCACCAC GCTGGAAGAA
    CTTGGTTCAC CTTCGAATCT GGTAACTTCA GACGTCACTG AGAACAGTTT TGTAGCATCC
    TGGACCGCCG CCCCTGGAAA CGTCCGTCTT TATCGGGTCA CCTGGAAATC CCTCTTTTCA
    GAGGAAGCTG GCGAGAAGAC TGTCCCCGGG GACGTAACCA CCACTGTTCT GGACGGGCTG
    ACACCAGAGA CCCGCTACAA GGTTTCTGTC TACGCTGTCT ACAGCCGTGC GGAGGGGGCG
    CCACTGCACG GAGAGGAGAC CACAGATGTG TCGGCCGCTA CCAGAGCTCT TACGGTGTCA
    GAAGAAACAG AGAGGACCAT GAAGGTGACA TGGATACCAG CACCTGGCAA TGTTGTCAAT
    TACAGGGTAT CATACAAACC TCAGGGTGAT TTACGCCAAA TGGCCTCCAA AGCACCAGGA
    GGCACCAATT CTATAGTCCT GAGACGCCTG ATCCCCCAGA CCACTTACGA TATCACAGTG
    GTTCCCGTCT ACAAGTTTGG GGATGGAAGA CAAAGGCAAG GAGAAGGAAC TACATTGTCT
    CAATATAAAG CTCCCAGAAA CTTGCAAACT TCGGAGCCCA CTAAAACCAG TTTCAGGGTG
    ACCTGGGATC ATGCTCCTGG AGATGTAAAA GGCTACAAGG TCACTTTCCA TCCCACCGGA
    AAGGAAGTGG ACCTGGGAGA GTTACTCGTA GGCCCCTATG ACAACACTGT GGTTCTGGAG
    GAACTAAGGG CCGGCACTAA GTATACGGTA AATGTGTTTG GTATGTTTGA TGGAGGAGAG
    AGTATGCCAT TGGCTGGAGA GGAGAAGACC ACCCTCTCTG ACGCAGCTTC TGAAAACCTC
    CCTTATACAC CTTCAGATGC CCAGTGTAAA ACCACGGCCC AAGCGGATAT CGTCCTGCTT
    GTTGATGGAT CTTGGAGCAT TGGTCGCCTG AACTTCAAGA CCATACGCAA TTTCATTGCT
    CGCATGGTGG GAGTCTTCGA CATCGGCCCC GATCGTGTCC AGATTGGTCT GGCACAGTAC
    AGCGGAGACC CGAAGACTGA GTGGCATCTC AATGCTCACA GAACCAGGAC TCAGCTGCTG
    GATGCGGTGG CCAATCTACC CTACAAAGGA GGAAACACTC TCACCGGCCT TGCTTTGAAT
    TACATCCTTC AGAATAACTT CAAGCCTAAC GTTGGCATGC GTCCAAACTC CAGAAAGATC
    GGCGTCCTGG TCACTGATGG CAAGTCCCAA GACGACATTG TGGCGAACTC ACAGAACCTG
    CGGGACCAAG GGATTGAACT GTACGCCATT GGTGTAAAGA ACGCAGATGA GAATGAGTTG
    AGAACCATTG CTTCCGATCC CGATGACATT CACATGTACA ACGTGGCTGA TTTCTCCTTC
    CTGCTGGACA TTGTGGATGA CCTCACAAAC AACCTGTGCA ACAGCGTCAA AGGTCCAGGA
    GGAGGACCAG AGCCACCCAC TGATCTGACC ACCTCAGAGG TCACACATTA CTCTTTCCGT
    GCCACTTGGG TGGCTCCTGA CGGGCCTGTG GAGAAGTACA GAGTGGAGTA TGTGAGCACC
    GCTGGAGGAG AACCACAGCA ACAGATGTTT GTGGACGGGA CGGTGACCAC TGTGGTTTTG
    GAAGGTCTCA CTCCGCTGAC CGAGTACATA GTAAAGGTTT ACTCAGTGGT GGGAGAGGAC
    AGCAGCGACC CACTCAGAGG CATAGAGACC ACATTGCCTC TTCCTCCATC CGGTGAGTTG
    AGGATTACCG ATGTGACCCA CAGCACCATG CAGCTGGACT GGGACGCTGC GCCCGGCGCC
    GTCCGAAAGT ACATCATCAC TTACAAGCCA GAGGAGGGAG AGGCGAAGGA GCTTGAAGTT
    GACGGAGATG TAACATCTAG AGGCCTGGAT AATCTCATCT CCCAGACTGA ATATGATGTG
    GCTGTGACTC CGGTTTATGA TTCGGGCACT GGAAACCCCA TGCTTAACCA GGCCATTACA
    GATGTGGTTC CTGCCCCGCA GAACTTGAGG TTCTCGATGG TGACCCAGAC CAGTTTTAGG
    GTTCACTGGG AGCACGGCGC TCCTGATGTC TCCCTCTACC GTATTGGCTG GAAAAAGAAA
    GGAACGACCG AGTTTCAAAA TGATATCCTG AGTAGTGAAG ACACTACATA TGATCTGGTG
    GACCTGGAGC CAGACACACC TTATGATGTT TCGGTCACTG CCATTTACCC TGATGAGTCT
    GAGAGCGAGG ATCTGCTGGG CACTGAACGC ACTTTGGCCA AGCTTGCTAC ACCACCTCCC
    AGTGGTCCTC CTCAAAACCT CCAGGTGTTC GACGAGACCA CCACCTCCCT CACGACTAAG
    TGGGACCACG CGCCTGGACG AGTTCAGAAC TACAAAATCA CCTACGTACC GCTTACTGGA
    GGACGATCAC AGTCGACGCA GGTCGGAGGA AAGAAAAACA CAGTGATCCT GCAGAAACTG
    ACTCCAGACA CTCCTTACTC CATCACTGTT GCTGCGGTCT ACGCCAGCGG AGAGGCCAGA
    GACATTTCTG GCCAAGGAAA GACATTGCCG CTGGGCAGTG TCAGGAATCT TCAGGTCACT
    GACCCCACAG TTAGCACTCT GAATGTGCGC TGGGAGCCGG CGGAGGGAAG CGTTCGCCAG
    TACAGGATCT ACTACCAGCC CGTCGCAGGG GGCGCAGAGG ACATGGTCCA GGTTCCTGGA
    AGCACCACCA GCACCGTTCT GAAGAACCTG CAATCTGACA CTCAGTACAG AGTGACGGTG
    GTGCCAGTCT ACGCTCCTGG AGAGGGCAAG CGCATGTCGG AGACCGGAAA AACACTGGTT
    CGGACCCCTG CCAAGAACAT CCAGGTTTAC AACCCCACCA CAAACACCCT GAACGTGCGC
    TGGGAGCCGG CCTCAGGACA GGTGCAGCAG TACCGCGTGG CTTACGCCCC TCTCTCCGGG
    ATCCGACCCT CTCAGTCGGT GCTGGTTCCA GGAAACACCC ACACGGCTCT GCTGCAGCAG
    CTGGAATCAG ACACTGACTA CTCTGTGAGT GTGGTGGCTC TGTATGCTGA TGGAGAGGGC
    TCGTCTGTGT CTGATAATGG CAAAACACTG CCCCGCAGTG GCCCCAGGAA CCTGCGGGTT
    TATGATCCCA CCACCAGCAC GCTGTCGGTG GGCTGGGAAC ATGCAGAAGG GCCTGTGATT
    CAGTACCGCA TTGGCTACGC ACCCACCACT GGAGATCCCA TTGAAGAATT TACAACTGTC
    CCAGGCAACC GTAACTCGGT CATCCTGCAA AACCTGCAGC CAGACACGCC GTACAACATC
    AACGTAGAAG CCATTTATCA AGATGGCCCT GGAGGACGAC TCAGTGGAGA TGGAAAAACA
    TTGGATCTCC TTGGGCCTCG TAACCTGCGT GTTTCAGACG AGTGGTACAC ACGTTTCCGA
    GTGTCCTGGG ATCCTGCAGC CGCACGGGTG AATGGATATA AACTGGTCTA TACACCAGTG
    GGCACAACCG ACACCATGGA AGCCTTCGTT GGAGACGTGA CTTCACACCA CCTGAACAAC
    CTCCAGCCTG GCACCACATA CGACCTGAAG GTTTACGCTC AGTATGATGC GGGCTTGAGC
    GGCGCTCTGG TCGGACAGGG AACCACCCTG TATCTCAACG TGACCGACCT CACTTCCTAC
    AACGTGGGCT ACGACTCTTT CTGCATCAGA TGGACTCCTC ATCGAGCTGC CACCTCCTAC
    AGACTCAAAG TCAACCCTTT CAACCCATCT CACCGTGGAG CTCAGGAGGT CACGATCACT
    GCGGCTGAGA GCCAGTACTG TTTTGACGGC CTGACGCCAG ACGCGCTATA CAACGCCACC
    GTCTACACGC AGACCCCCAA CCGAGAGGGG CCCGGAGTCA GTGTCAAAGA GCGCACACTG
    GTGAAGCCCA CCGTCGCCCC AACACTACCT CCAACTCCTC CACCTCCAGC CACCATTCCT
    TCTGCACTCG AGGTGTGCAA AGGTGCCAAG GCTGACCTGG TGTTCCTGAT CGATGGCTCG
    TGGAGTATCG GAGATGACAG CTTCGCTAAA GTCCGGCAGT TTGTGTTCAG CATGATTGGG
    GCGTTTGATG TGGTCAGCCA TGAGGGCATG CAGGTGTCTT TCGTGCAGTA CAGCGACGAC
    GCCAAAACTG AGTTCAAGCT CAACACCTAC CACGACAAGG GAATGGTGCT GTCTGCTCTT
    CAGATGGTCA ATTATAGAGG AGGAAACACC AAAACAGGTG CTGCACTGAA GCATGTTGGA
    GAAAAAGTGT TCACCTCGGA CAATGGCATG AGGAGAACCG TTCCCAAGGT ACTGGTTGTG
    GTGACCGATG GACGTTCTCA AGATGACGTG AAGAAGAGCG CTGCTAAACT ACAGCATGCA
    GGTTACAGTG TGTTTGTGGT GGGTGTCGCT GACATTGACG TTAATGAACT GAGGAACATT
    GGAAGCAAGC CCAGTGACAG ACACGTCTTT ATCGTCGACA ACTTCGATGC TTTCGCCAAG
    ATCCAAGACA ACCTGATCAC GTTCATCTGT GAAACGGCAA CGTCCACATG CCCTTTGATT
    TACCTCAATG GATTCACATC ACCAGGCTTC AGGATGCTGG AAGCTTTCAA CATCACTGAC
    AAGACCTACG CAGGCATGAA CGGCGTCTCT CTGGAAACCG GCTCCTTCAA CAGCTTCATC
    GCATACAGAC TCCATAAGGA CGCCTTCATT TCCCAGCCAA CAAAGGAAAT CCATCCAGAT
    GGTCTTCCTC CATCCTACAC CCTCATCCTC CTCTTCCGTC TGCTCCCGGA CACCGGCAAG
    GATGCGTTTG ATTTATGGCA GATCTCAGAT AAGAACAATA ACCCTGAGGT CGGGCTCACC
    ATTGACCCCT CCAGCAGAGT GATCTCCTTC TACAATAAAG ACACTAGAGG GGAAATTCAG
    AAAGTCTCCT TTGATAACGA CCACGTGAAG AAGATTTTCC ACGGCAGTTT CCACAAGCTT
    CACATTTCTA TTTCGCCGAA AAATGTCAAA CTCAACATCG ACTGCCAAGA GGTGGCTGAG
    AAACAGATCA AAGCAGCCAA CAACGTCTCC ACAGATGGCT ACGAAGTCCT TGGAAAAATG
    ACAAAGTCTG GCGGTTCAAA GAAGCAGTCG GCCACATTCC AGCTCCAGAT GTTTGATATT
    GTCTGCAGTT TGGGCTGGAC AAGTCGAGAC AAATGTTGTG ATATTCCAGC AATGCGAGAT
    GAAGCCAAGT GTCCTGCTCT TCCTCACTCC TGCACATGTG CTCAGGACAG TGTTGGTCCT
    CCAGGCCCAG CGGGTCCTCA AGGCAGTCCT GGTACAAAAG GCCCACGAGG CGAGAGAGGA
    GAGTCTGGTC CTACGGGTCC AATGGGTCCA CGTGGAGAGC AGGGTCTGCC AGGGCAGATG
    GGGCCACCGG GTCCACAGGG CCCCAATGGA CTATCCCTTC CCGGAGAACC AGGGCGACCG
    GGACCTAAGG GAGACCCCGG TGACTCAGGT TTACCTGGAC AGAGAGGCCC GGTTGGCGCT
    GCAGGTCCAC TCGGCCCTGT TGGACCCGCT GGAGCAAGAG GTCCACCAGG AAGTGAAGGC
    CCATCTGGAC CAAGAGGTCC ATCGGGACCA ATGGGACCAC CGGGAGCTCC CGGAATGCCA
    GGAATCACCG GAAAACCAGG AAAACCCGGA GACACTGGCT TTCCTGGACC TGTTGGCATG
    AAAGGAGAGA AGGGTGAAAG AGGAGACACT GCGTCACAGA ATATGATGCG CTCTGTAGCG
    AGACAAGTCT GCGAACAGCT AGTCAGCAGA CAAATGAGCA GAATTGATAT GATGATGAAC
    CAGATCCCCA GCAACTACAG GAGTAACAGC CCCGGCCCCG CTGGACCTCC AGGCCCACCT
    GGAAACCAGG GACCCCATGG AGAGCCTGGT CAGCCCGGAC CCAATGGCTT CCCAGGAAAT
    CCAGGTTTAC CAGGCTCTCC TGGAGAAAGA GGAGTGCCTG GAGAGAAGGG TGAACGAGGC
    AGCCCCGGCA TTGGCACTCG AGGACAGAGA GGATTACCTG GACCTCCTGG GCCACCAGGG
    CAGTCTCAGA CCGGTCCCCC TGGTCCCTCA GGCTCACCCG GGCCCCGAGG ACCACCAGGA
    CAACATGGTT CTTCTGGAGT CACAGGACCA CCAGGACCTC CAGGATACTG TGACTCCTCT
    CAGTGCGGCA TGCCTTACAA CGGACAAGGC TACCCAGGCC CCTACCAGCC CGGGTCTGAA
    ACCTACGTAG TGCCCATACC AGCCGCGCCA TCGGAAGATA TCGAGACACA GTCGCCAGGA
    TTGCGCTACC AGCGCGGAAA AAGGTCGCTA TCAAGAAAGG AAGCCGAAAG AATCAAAACA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009291572.1
    CDS179..9241
    Translation

    Target ORF information:

    RefSeq Version XM_009293297.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio collagen, type XII, alpha 1a (col12a1a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009293297.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    ATGAAGATCA GGTTGTCTTT GGCCGCTGCT GCGCTCTTGA CTCTAGTACT CAGTTCAGTA 
    GAGGCTCAAG TGGAGCCTCC GTCAGACTTA AAATTTAAAA TCCTGAATGA AAACACAGTG
    CAAATGTCAT GGAGGAAGCC CTCGTCTCAG ATACAGGGCT ACAGAATACA GGTCACGTCA
    GATACAGATG ATTCTAAAGA ATTGAGTCTT CCTGCCTCGT CCACAAGCAC ATCAATTACA
    GATCTAACTC CAGATGTAGA CTATTCAGTG TCAATCAATT CATATGATGG TGCTGAAGAA
    AGTATCCCTA TCCTTGGACA AATCACAATT CAATCCAGCA ACACAAGTGG ACCAAGGAGG
    CCACAGTCAG AGGCTGTCAA GTGTTCAGTG AGTGCCGTTG CGGATCTGGT TTTCCTGGTG
    GACGGCTCTT GGAGTGTGGG AAGAGAAAAC TTTAGATTTA TCCGGAGTTT CATTGGGGCC
    ATGGCCGGGG CCTTCGACAT TGAAGAGGAT AAGACCAGAG TGGCTGTGGT TCAGTACAGC
    TCGGACACCA GGACAGAGTT CAGTCTAAAC ACACATTTCA GACGACCCGA TGTCCTGCGA
    GCCATTAAAA ATCTGCCGTA CAAGGGTGGA AATACCATGA CGGGTGATGC AATGGACTAT
    CTGGTGAAAA ACACGTTCAC TCAGGCGGCT GGAGCCAGGA AAGGCTTCCC AAAAGTGGCC
    ATGATCATCA CAGATGGAAA ATCTCAAGAC CCCGTAGAGG AGTACGCCGA ACGGTTGAGG
    AACATCGGAG TGGAGATCTT TGTGCTGGGT ATCAAAGGAG CAGATGAGGA CGAGCTGAAG
    GAGATCGCCT CCAGACCCCA TAGCAAGCAT GTCTATAACG TCCCCAACTT CGACATGATC
    AATCAGGTCC AGAAGGCGCT CATCACTGAA GTTTGCTCTG GCGTGGAGGA GCAGCTAAAT
    TCCCTTGTAA GCGGGGAGGA AGTTGTCGAA CCAGCTTCCA ACCTCAGAGT GACAGATATC
    TCCTCCAAAT CCATGAGAAT TGCGTGGGAT TCATCTCCTG GTGATATCAC AGGCTATAAG
    CTGCAGGTTT TTCCCACTAT TGCGAGCGCC AAAAAGCAGG AGCTCTACAC TGGGCCCACG
    ATGACCTTGG CCAATGTCAG AGACCTGACG CCTGAGATGG AGTATGAGAT CAGTCTGTTT
    GCTCTCAAAG GTCTGACTCC TAGTGTGCCG CAAGTTGTCA CTGAGAAAAC TCAGCCAGTG
    AAAGTATTTA CAGAGTGTTC CCTAGAGGTG GATGTCCAAG CTGACATTGT CCTCCTGGTG
    GATGGGTCAT ATAGTATAGG ACTGCCTAAC TTTGCGAAGG TTAGAGCTTT CCTCGAAGTA
    CTTGTGAATT CTTTTGACAT CGGACCTAAC AAAGTGCAGA TCAGCTTGGT TCAGTACAGC
    AGGGATCCTC ACACTGAGTT CGCCTTGAAT AAATTCGATG ACAATGCAGC CATGGTCAAG
    GCGGTCCGCA CCTTCCCCTA CCGAGGTGGA TCCACCAACA CCGGCAAGGC CATGACATAT
    GTGAGGGAAA AGATCTTTGT TTCGGGCCGT GGCGCTCGTG ATAACGTCCC CCGAGTCATG
    GTGCTTATCA CAGACGGCAA GTCCTCAGAT TCTTTCAAAG ACCCCGCCAA CAAACTGAGG
    GATACAGATG TGGAGATTTT TGCAGTGGGA GTAAAAGATG CCGTACGATC TGAGCTGGAG
    GCTATCGCCA ATGTTCCTGC TGATAACCAT GTGTTTGAGG TGGAGGACTT TGATGCTTTC
    GAGCGGATCT CCAAGGAGCT GACCGCGTCC ATCTGCCTCA GAATTGAGCA GGAGCTCCAT
    AACATCAGGA AAAGAAGTTT GCTTCCCCCA AAGTCACTGA GCTTCTCTGA GGTGACCTCC
    AGGAGTTTCA GGACCTCATG GGTTTCTGAT GGAGAGGATA TCCTGTCTTT TCTGGTGCGC
    TTCAGGCCAG CGGCGGACCT AACTGGAGAC TACATCTCTC TGGTGGTTCC CCATGACATC
    ACCAGTACAA TTCTGCCCAA TCTCACGCCA CTCACCACCT ACGAAGTTAA CGTCATTGCT
    CAGTATGATA AAGGAGACAG CTTTCCTTTG ACGGGCGAGG AGACCACTTT AGAAGAGTTG
    GGTTCTGTTC GTAACCTGAT GGTGAGCGAG GAGGCTGTGG ACCGCTTTCG GGTGTCGTGG
    AGAGCTGCTC CAGGATTAGT GCTGAGGTAC AGACTGCTCT ACGAGCCTGT GAGCGGAGGG
    GATAAACTGG AAGCAGAGAC TGATGGGAAT CAGGTCACCA CTGTGCTCAA TGAACTACTA
    CCGGTCACTA CTTACCGGGT CACCGTTTAC CCTGAGTACG CAAGTGGAAG GGGTCCCTCC
    ATGGACACTG AAGGCACCAC CAAAGAAGTT CGAGGTTCTC CACGCGACTT AAGGGTCTTC
    AATGAGACGG TGTCTAGCAT GAGGGTCTCG TGGCGGGCTG CCCCAGGCAA CGTGCTTCAA
    TATCAGGTGG CCTACAAACC TGACGGTGGG GAGAGGAAAG AAATAAGTGT TAAAGGAGAG
    ACAACAACTG CTGTGCTGAA AAATCTGCTG CCGGACACGG AGTATGAGCT GTTTGTTAGC
    GCACGTTACG TCTCTGGACT TGGCGCACCA CTTATGGGAA CAGGCACCAC GCTGGAAGAA
    CTTGGTTCAC CTTCGAATCT GGTAACTTCA GACGTCACTG AGAACAGTTT TGTAGCATCC
    TGGACCGCCG CCCCTGGAAA CGTCCGTCTT TATCGGGTCA CCTGGAAATC CCTCTTTTCA
    GAGGAAGCTG GCGAGAAGAC TGTCCCCGGG GACGTAACCA CCACTGTTCT GGACGGGCTG
    ACACCAGAGA CCCGCTACAA GGTTTCTGTC TACGCTGTCT ACAGCCGTGC GGAGGGGGCG
    CCACTGCACG GAGAGGAGAC CACAGATGTG TCGGCCGCTA CCAGAGCTCT TACGGTGTCA
    GAAGAAACAG AGAGGACCAT GAAGGTGACA TGGATACCAG CACCTGGCAA TGTTGTCAAT
    TACAGGGTAT CATACAAACC TCAGGGTGAT TTACGCCAAA TGGCCTCCAA AGCACCAGGA
    GGCACCAATT CTATAGTCCT GAGACGCCTG ATCCCCCAGA CCACTTACGA TATCACAGTG
    GTTCCCGTCT ACAAGTTTGG GGATGGAAGA CAAAGGCAAG GAGAAGGAAC TACATTGTCT
    CAATATAAAG CTCCCAGAAA CTTGCAAACT TCGGAGCCCA CTAAAACCAG TTTCAGGGTG
    ACCTGGGATC ATGCTCCTGG AGATGTAAAA GGCTACAAGG TCACTTTCCA TCCCACCGGA
    AAGGAAGTGG ACCTGGGAGA GTTACTCGTA GGCCCCTATG ACAACACTGT GGTTCTGGAG
    GAACTAAGGG CCGGCACTAA GTATACGGTA AATGTGTTTG GTATGTTTGA TGGAGGAGAG
    AGTATGCCAT TGGCTGGAGA GGAGAAGACC ACCCTCTCTG ACGCAGCTTC TGAAAACCTC
    CCTTATACAC CTTCAGATGC CCAGTGTAAA ACCACGGCCC AAGCGGATAT CGTCCTGCTT
    GTTGATGGAT CTTGGAGCAT TGGTCGCCTG AACTTCAAGA CCATACGCAA TTTCATTGCT
    CGCATGGTGG GAGTCTTCGA CATCGGCCCC GATCGTGTCC AGATTGGTCT GGCACAGTAC
    AGCGGAGACC CGAAGACTGA GTGGCATCTC AATGCTCACA GAACCAGGAC TCAGCTGCTG
    GATGCGGTGG CCAATCTACC CTACAAAGGA GGAAACACTC TCACCGGCCT TGCTTTGAAT
    TACATCCTTC AGAATAACTT CAAGCCTAAC GTTGGCATGC GTCCAAACTC CAGAAAGATC
    GGCGTCCTGG TCACTGATGG CAAGTCCCAA GACGACATTG TGGCGAACTC ACAGAACCTG
    CGGGACCAAG GGATTGAACT GTACGCCATT GGTGTAAAGA ACGCAGATGA GAATGAGTTG
    AGAACCATTG CTTCCGATCC CGATGACATT CACATGTACA ACGTGGCTGA TTTCTCCTTC
    CTGCTGGACA TTGTGGATGA CCTCACAAAC AACCTGTGCA ACAGCGTCAA AGGTCCAGGA
    GGAGGACCAG AGCCACCCAC TGATCTGACC ACCTCAGAGG TCACACATTA CTCTTTCCGT
    GCCACTTGGG TGGCTCCTGA CGGGCCTGTG GAGAAGTACA GAGTGGAGTA TGTGAGCACC
    GCTGGAGGAG AACCACAGCA ACAGATGTTT GTGGACGGGA CGGTGACCAC TGTGGTTTTG
    GAAGGTCTCA CTCCGCTGAC CGAGTACATA GTAAAGGTTT ACTCAGTGGT GGGAGAGGAC
    AGCAGCGACC CACTCAGAGG CATAGAGACC ACATTGCCTC TTCCTCCATC CGGTGAGTTG
    AGGATTACCG ATGTGACCCA CAGCACCATG CAGCTGGACT GGGACGCTGC GCCCGGCGCC
    GTCCGAAAGT ACATCATCAC TTACAAGCCA GAGGAGGGAG AGGCGAAGGA GCTTGAAGTT
    GACGGAGATG TAACATCTAG AGGCCTGGAT AATCTCATCT CCCAGACTGA ATATGATGTG
    GCTGTGACTC CGGTTTATGA TTCGGGCACT GGAAACCCCA TGCTTAACCA GGCCATTACA
    GATGTGGTTC CTGCCCCGCA GAACTTGAGG TTCTCGATGG TGACCCAGAC CAGTTTTAGG
    GTTCACTGGG AGCACGGCGC TCCTGATGTC TCCCTCTACC GTATTGGCTG GAAAAAGAAA
    GGAACGACCG AGTTTCAAAA TGATATCCTG AGTAGTGAAG ACACTACATA TGATCTGGTG
    GACCTGGAGC CAGACACACC TTATGATGTT TCGGTCACTG CCATTTACCC TGATGAGTCT
    GAGAGCGAGG ATCTGCTGGG CACTGAACGC ACTTTGGCCA AGCTTGCTAC ACCACCTCCC
    AGTGGTCCTC CTCAAAACCT CCAGGTGTTC GACGAGACCA CCACCTCCCT CACGACTAAG
    TGGGACCACG CGCCTGGACG AGTTCAGAAC TACAAAATCA CCTACGTACC GCTTACTGGA
    GGACGATCAC AGTCGACGCA GGTCGGAGGA AAGAAAAACA CAGTGATCCT GCAGAAACTG
    ACTCCAGACA CTCCTTACTC CATCACTGTT GCTGCGGTCT ACGCCAGCGG AGAGGCCAGA
    GACATTTCTG GCCAAGGAAA GACATTGCCG CTGGGCAGTG TCAGGAATCT TCAGGTCACT
    GACCCCACAG TTAGCACTCT GAATGTGCGC TGGGAGCCGG CGGAGGGAAG CGTTCGCCAG
    TACAGGATCT ACTACCAGCC CGTCGCAGGG GGCGCAGAGG ACATGGTCCA GGTTCCTGGA
    AGCACCACCA GCACCGTTCT GAAGAACCTG CAATCTGACA CTCAGTACAG AGTGACGGTG
    GTGCCAGTCT ACGCTCCTGG AGAGGGCAAG CGCATGTCGG AGACCGGAAA AACACTGGTT
    CGGACCCCTG CCAAGAACAT CCAGGTTTAC AACCCCACCA CAAACACCCT GAACGTGCGC
    TGGGAGCCGG CCTCAGGACA GGTGCAGCAG TACCGCGTGG CTTACGCCCC TCTCTCCGGG
    ATCCGACCCT CTCAGTCGGT GCTGGTTCCA GGAAACACCC ACACGGCTCT GCTGCAGCAG
    CTGGAATCAG ACACTGACTA CTCTGTGAGT GTGGTGGCTC TGTATGCTGA TGGAGAGGGC
    TCGTCTGTGT CTGATAATGG CAAAACACTG CCCCGCAGTG GCCCCAGGAA CCTGCGGGTT
    TATGATCCCA CCACCAGCAC GCTGTCGGTG GGCTGGGAAC ATGCAGAAGG GCCTGTGATT
    CAGTACCGCA TTGGCTACGC ACCCACCACT GGAGATCCCA TTGAAGAATT TACAACTGTC
    CCAGGCAACC GTAACTCGGT CATCCTGCAA AACCTGCAGC CAGACACGCC GTACAACATC
    AACGTAGAAG CCATTTATCA AGATGGCCCT GGAGGACGAC TCAGTGGAGA TGGAAAAACA
    TTGGATCTCC TTGGGCCTCG TAACCTGCGT GTTTCAGACG AGTGGTACAC ACGTTTCCGA
    GTGTCCTGGG ATCCTGCAGC CGCACGGGTG AATGGATATA AACTGGTCTA TACACCAGTG
    GGCACAACCG ACACCATGGA AGCCTTCGTT GGAGACGTGA CTTCACACCA CCTGAACAAC
    CTCCAGCCTG GCACCACATA CGACCTGAAG GTTTACGCTC AGTATGATGC GGGCTTGAGC
    GGCGCTCTGG TCGGACAGGG AACCACCCTG TATCTCAACG TGACCGACCT CACTTCCTAC
    AACGTGGGCT ACGACTCTTT CTGCATCAGA TGGACTCCTC ATCGAGCTGC CACCTCCTAC
    AGACTCAAAG TCAACCCTTT CAACCCATCT CACCGTGGAG CTCAGGAGGT CACGATCACT
    GCGGCTGAGA GCCAGTACTG TTTTGACGGC CTGACGCCAG ACGCGCTATA CAACGCCACC
    GTCTACACGC AGACCCCCAA CCGAGAGGGG CCCGGAGTCA GTGTCAAAGA GCGCACACTG
    GTGAAGCCCA CCGTCGCCCC AACACTACCT CCAACTCCTC CACCTCCAGC CACCATTCCT
    TCTGCACTCG AGGTGTGCAA AGGTGCCAAG GCTGACCTGG TGTTCCTGAT CGATGGCTCG
    TGGAGTATCG GAGATGACAG CTTCGCTAAA GTCCGGCAGT TTGTGTTCAG CATGATTGGG
    GCGTTTGATG TGGTCAGCCA TGAGGGCATG CAGGTGTCTT TCGTGCAGTA CAGCGACGAC
    GCCAAAACTG AGTTCAAGCT CAACACCTAC CACGACAAGG GAATGGTGCT GTCTGCTCTT
    CAGATGGTCA ATTATAGAGG AGGAAACACC AAAACAGGTG CTGCACTGAA GCATGTTGGA
    GAAAAAGTGT TCACCTCGGA CAATGGCATG AGGAGAACCG TTCCCAAGGT ACTGGTTGTG
    GTGACCGATG GACGTTCTCA AGATGACGTG AAGAAGAGCG CTGCTAAACT ACAGCATGCA
    GGTTACAGTG TGTTTGTGGT GGGTGTCGCT GACATTGACG TTAATGAACT GAGGAACATT
    GGAAGCAAGC CCAGTGACAG ACACGTCTTT ATCGTCGACA ACTTCGATGC TTTCGCCAAG
    ATCCAAGACA ACCTGATCAC GTTCATCTGT GAAACGGCAA CGTCCACATG CCCTTTGATT
    TACCTCAATG GATTCACATC ACCAGGCTTC AGGATGCTGG AAGCTTTCAA CATCACTGAC
    AAGACCTACG CAGGCATGAA CGGCGTCTCT CTGGAAACCG GCTCCTTCAA CAGCTTCATC
    GCATACAGAC TCCATAAGGA CGCCTTCATT TCCCAGCCAA CAAAGGAAAT CCATCCAGAT
    GGTCTTCCTC CATCCTACAC CCTCATCCTC CTCTTCCGTC TGCTCCCGGA CACCGGCAAG
    GATGCGTTTG ATTTATGGCA GATCTCAGAT AAGAACAATA ACCCTGAGGT CGGGCTCACC
    ATTGACCCCT CCAGCAGAGT GATCTCCTTC TACAATAAAG ACACTAGAGG GGAAATTCAG
    AAAGTCTCCT TTGATAACGA CCACGTGAAG AAGATTTTCC ACGGCAGTTT CCACAAGCTT
    CACATTTCTA TTTCGCCGAA AAATGTCAAA CTCAACATCG ACTGCCAAGA GGTGGCTGAG
    AAACAGATCA AAGCAGCCAA CAACGTCTCC ACAGATGGCT ACGAAGTCCT TGGAAAAATG
    ACAAAGTCTG GCGGTTCAAA GAAGCAGTCG GCCACATTCC AGCTCCAGAT GTTTGATATT
    GTCTGCAGTT TGGGCTGGAC AAGTCGAGAC AAATGTTGTG ATATTCCAGC AATGCGAGAT
    GAAGCCAAGT GTCCTGCTCT TCCTCACTCC TGCACATGTG CTCAGGACAG TGTTGGTCCT
    CCAGGCCCAG CGGGTCCTCA AGGCAGTCCT GGTACAAAAG GCCCACGAGG CGAGAGAGGA
    GAGTCTGGTC CTACGGGTCC AATGGGTCCA CGTGGAGAGC AGGGTCTGCC AGGGCAGATG
    GGGCCACCGG GTCCACAGGG CCCCAATGGA CTATCCCTTC CCGGAGAACC AGGGCGACCG
    GGACCTAAGG GAGACCCCGG TGACTCAGGT TTACCTGGAC AGAGAGGCCC GGTTGGCGCT
    GCAGGTCCAC TCGGCCCTGT TGGACCCGCT GGAGCAAGAG GTCCACCAGG AAGTGAAGGC
    CCATCTGGAC CAAGAGGTCC ATCGGGACCA ATGGGACCAC CGGGAGCTCC CGGAATGCCA
    GGAATCACCG GAAAACCAGG AAAACCCGGA GACACTGGCT TTCCTGGACC TGTTGGCATG
    AAAGGAGAGA AGGGTGAAAG AGGAGACACT GCGTCACAGA ATATGATGCG CTCTGTAGCG
    AGACAAGTCT GCGAACAGCT AGTCAGCAGA CAAATGAGCA GAATTGATAT GATGATGAAC
    CAGATCCCCA GCAACTACAG GAGTAACAGC CCCGGCCCCG CTGGACCTCC AGGCCCACCT
    GGAAACCAGG GACCCCATGG AGAGCCTGGT CAGCCCGGAC CCAATGGCTT CCCAGGAAAT
    CCAGGTTTAC CAGGCTCTCC TGGAGAAAGA GGAGTGCCTG GAGAGAAGGG TGAACGAGGC
    AGCCCCGGCA TTGGCACTCG AGGACAGAGA GGATTACCTG GACCTCCTGG GCCACCAGGG
    CAGTCTCAGA CCGGTCCCCC TGGTCCCTCA GGCTCACCCG GGCCCCGAGG ACCACCAGGA
    CAACATGGTT CTTCTGGAGT CACAGGACCA CCAGGACCTC CAGGATACTG TGACTCCTCT
    CAGTGCGGCA TGCCTTACAA CGGACAAGGC TACCCAGGCC CCTACCAGCC CGGGTCTGAA
    ACCTACGTAG TGCCCATACC AGCCGCGCCA TCGGAAGATA TCGAGACACA GTCGCCAGGA
    TTGCGCTACC AGCGCGGAAA AAGGTCGCTA TCAAGAAAGG AAGCCGAAAG AATCAAAACA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48773
    Clone ID Related Accession (Same CDS sequence) XM_009293297.3 , XM_009293297.2
    Accession Version XM_009293297.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9063bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product collagen alpha-1(XII) chain isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293297.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
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    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
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    3721
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    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
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    4621
    4681
    4741
    4801
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    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
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    5521
    5581
    5641
    5701
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    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
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    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
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    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    ATGAAGATCA GGTTGTCTTT GGCCGCTGCT GCGCTCTTGA CTCTAGTACT CAGTTCAGTA 
    GAGGCTCAAG TGGAGCCTCC GTCAGACTTA AAATTTAAAA TCCTGAATGA AAACACAGTG
    CAAATGTCAT GGAGGAAGCC CTCGTCTCAG ATACAGGGCT ACAGAATACA GGTCACGTCA
    GATACAGATG ATTCTAAAGA ATTGAGTCTT CCTGCCTCGT CCACAAGCAC ATCAATTACA
    GATCTAACTC CAGATGTAGA CTATTCAGTG TCAATCAATT CATATGATGG TGCTGAAGAA
    AGTATCCCTA TCCTTGGACA AATCACAATT CAATCCAGCA ACACAAGTGG ACCAAGGAGG
    CCACAGTCAG AGGCTGTCAA GTGTTCAGTG AGTGCCGTTG CGGATCTGGT TTTCCTGGTG
    GACGGCTCTT GGAGTGTGGG AAGAGAAAAC TTTAGATTTA TCCGGAGTTT CATTGGGGCC
    ATGGCCGGGG CCTTCGACAT TGAAGAGGAT AAGACCAGAG TGGCTGTGGT TCAGTACAGC
    TCGGACACCA GGACAGAGTT CAGTCTAAAC ACACATTTCA GACGACCCGA TGTCCTGCGA
    GCCATTAAAA ATCTGCCGTA CAAGGGTGGA AATACCATGA CGGGTGATGC AATGGACTAT
    CTGGTGAAAA ACACGTTCAC TCAGGCGGCT GGAGCCAGGA AAGGCTTCCC AAAAGTGGCC
    ATGATCATCA CAGATGGAAA ATCTCAAGAC CCCGTAGAGG AGTACGCCGA ACGGTTGAGG
    AACATCGGAG TGGAGATCTT TGTGCTGGGT ATCAAAGGAG CAGATGAGGA CGAGCTGAAG
    GAGATCGCCT CCAGACCCCA TAGCAAGCAT GTCTATAACG TCCCCAACTT CGACATGATC
    AATCAGGTCC AGAAGGCGCT CATCACTGAA GTTTGCTCTG GCGTGGAGGA GCAGCTAAAT
    TCCCTTGTAA GCGGGGAGGA AGTTGTCGAA CCAGCTTCCA ACCTCAGAGT GACAGATATC
    TCCTCCAAAT CCATGAGAAT TGCGTGGGAT TCATCTCCTG GTGATATCAC AGGCTATAAG
    CTGCAGGTTT TTCCCACTAT TGCGAGCGCC AAAAAGCAGG AGCTCTACAC TGGGCCCACG
    ATGACCTTGG CCAATGTCAG AGACCTGACG CCTGAGATGG AGTATGAGAT CAGTCTGTTT
    GCTCTCAAAG GTCTGACTCC TAGTGTGCCG CAAGTTGTCA CTGAGAAAAC TCAGCCAGTG
    AAAGTATTTA CAGAGTGTTC CCTAGAGGTG GATGTCCAAG CTGACATTGT CCTCCTGGTG
    GATGGGTCAT ATAGTATAGG ACTGCCTAAC TTTGCGAAGG TTAGAGCTTT CCTCGAAGTA
    CTTGTGAATT CTTTTGACAT CGGACCTAAC AAAGTGCAGA TCAGCTTGGT TCAGTACAGC
    AGGGATCCTC ACACTGAGTT CGCCTTGAAT AAATTCGATG ACAATGCAGC CATGGTCAAG
    GCGGTCCGCA CCTTCCCCTA CCGAGGTGGA TCCACCAACA CCGGCAAGGC CATGACATAT
    GTGAGGGAAA AGATCTTTGT TTCGGGCCGT GGCGCTCGTG ATAACGTCCC CCGAGTCATG
    GTGCTTATCA CAGACGGCAA GTCCTCAGAT TCTTTCAAAG ACCCCGCCAA CAAACTGAGG
    GATACAGATG TGGAGATTTT TGCAGTGGGA GTAAAAGATG CCGTACGATC TGAGCTGGAG
    GCTATCGCCA ATGTTCCTGC TGATAACCAT GTGTTTGAGG TGGAGGACTT TGATGCTTTC
    GAGCGGATCT CCAAGGAGCT GACCGCGTCC ATCTGCCTCA GAATTGAGCA GGAGCTCCAT
    AACATCAGGA AAAGAAGTTT GCTTCCCCCA AAGTCACTGA GCTTCTCTGA GGTGACCTCC
    AGGAGTTTCA GGACCTCATG GGTTTCTGAT GGAGAGGATA TCCTGTCTTT TCTGGTGCGC
    TTCAGGCCAG CGGCGGACCT AACTGGAGAC TACATCTCTC TGGTGGTTCC CCATGACATC
    ACCAGTACAA TTCTGCCCAA TCTCACGCCA CTCACCACCT ACGAAGTTAA CGTCATTGCT
    CAGTATGATA AAGGAGACAG CTTTCCTTTG ACGGGCGAGG AGACCACTTT AGAAGAGTTG
    GGTTCTGTTC GTAACCTGAT GGTGAGCGAG GAGGCTGTGG ACCGCTTTCG GGTGTCGTGG
    AGAGCTGCTC CAGGATTAGT GCTGAGGTAC AGACTGCTCT ACGAGCCTGT GAGCGGAGGG
    GATAAACTGG AAGCAGAGAC TGATGGGAAT CAGGTCACCA CTGTGCTCAA TGAACTACTA
    CCGGTCACTA CTTACCGGGT CACCGTTTAC CCTGAGTACG CAAGTGGAAG GGGTCCCTCC
    ATGGACACTG AAGGCACCAC CAAAGAAGTT CGAGGTTCTC CACGCGACTT AAGGGTCTTC
    AATGAGACGG TGTCTAGCAT GAGGGTCTCG TGGCGGGCTG CCCCAGGCAA CGTGCTTCAA
    TATCAGGTGG CCTACAAACC TGACGGTGGG GAGAGGAAAG AAATAAGTGT TAAAGGAGAG
    ACAACAACTG CTGTGCTGAA AAATCTGCTG CCGGACACGG AGTATGAGCT GTTTGTTAGC
    GCACGTTACG TCTCTGGACT TGGCGCACCA CTTATGGGAA CAGGCACCAC GCTGGAAGAA
    CTTGGTTCAC CTTCGAATCT GGTAACTTCA GACGTCACTG AGAACAGTTT TGTAGCATCC
    TGGACCGCCG CCCCTGGAAA CGTCCGTCTT TATCGGGTCA CCTGGAAATC CCTCTTTTCA
    GAGGAAGCTG GCGAGAAGAC TGTCCCCGGG GACGTAACCA CCACTGTTCT GGACGGGCTG
    ACACCAGAGA CCCGCTACAA GGTTTCTGTC TACGCTGTCT ACAGCCGTGC GGAGGGGGCG
    CCACTGCACG GAGAGGAGAC CACAGATGTG TCGGCCGCTA CCAGAGCTCT TACGGTGTCA
    GAAGAAACAG AGAGGACCAT GAAGGTGACA TGGATACCAG CACCTGGCAA TGTTGTCAAT
    TACAGGGTAT CATACAAACC TCAGGGTGAT TTACGCCAAA TGGCCTCCAA AGCACCAGGA
    GGCACCAATT CTATAGTCCT GAGACGCCTG ATCCCCCAGA CCACTTACGA TATCACAGTG
    GTTCCCGTCT ACAAGTTTGG GGATGGAAGA CAAAGGCAAG GAGAAGGAAC TACATTGTCT
    CAATATAAAG CTCCCAGAAA CTTGCAAACT TCGGAGCCCA CTAAAACCAG TTTCAGGGTG
    ACCTGGGATC ATGCTCCTGG AGATGTAAAA GGCTACAAGG TCACTTTCCA TCCCACCGGA
    AAGGAAGTGG ACCTGGGAGA GTTACTCGTA GGCCCCTATG ACAACACTGT GGTTCTGGAG
    GAACTAAGGG CCGGCACTAA GTATACGGTA AATGTGTTTG GTATGTTTGA TGGAGGAGAG
    AGTATGCCAT TGGCTGGAGA GGAGAAGACC ACCCTCTCTG ACGCAGCTTC TGAAAACCTC
    CCTTATACAC CTTCAGATGC CCAGTGTAAA ACCACGGCCC AAGCGGATAT CGTCCTGCTT
    GTTGATGGAT CTTGGAGCAT TGGTCGCCTG AACTTCAAGA CCATACGCAA TTTCATTGCT
    CGCATGGTGG GAGTCTTCGA CATCGGCCCC GATCGTGTCC AGATTGGTCT GGCACAGTAC
    AGCGGAGACC CGAAGACTGA GTGGCATCTC AATGCTCACA GAACCAGGAC TCAGCTGCTG
    GATGCGGTGG CCAATCTACC CTACAAAGGA GGAAACACTC TCACCGGCCT TGCTTTGAAT
    TACATCCTTC AGAATAACTT CAAGCCTAAC GTTGGCATGC GTCCAAACTC CAGAAAGATC
    GGCGTCCTGG TCACTGATGG CAAGTCCCAA GACGACATTG TGGCGAACTC ACAGAACCTG
    CGGGACCAAG GGATTGAACT GTACGCCATT GGTGTAAAGA ACGCAGATGA GAATGAGTTG
    AGAACCATTG CTTCCGATCC CGATGACATT CACATGTACA ACGTGGCTGA TTTCTCCTTC
    CTGCTGGACA TTGTGGATGA CCTCACAAAC AACCTGTGCA ACAGCGTCAA AGGTCCAGGA
    GGAGGACCAG AGCCACCCAC TGATCTGACC ACCTCAGAGG TCACACATTA CTCTTTCCGT
    GCCACTTGGG TGGCTCCTGA CGGGCCTGTG GAGAAGTACA GAGTGGAGTA TGTGAGCACC
    GCTGGAGGAG AACCACAGCA ACAGATGTTT GTGGACGGGA CGGTGACCAC TGTGGTTTTG
    GAAGGTCTCA CTCCGCTGAC CGAGTACATA GTAAAGGTTT ACTCAGTGGT GGGAGAGGAC
    AGCAGCGACC CACTCAGAGG CATAGAGACC ACATTGCCTC TTCCTCCATC CGGTGAGTTG
    AGGATTACCG ATGTGACCCA CAGCACCATG CAGCTGGACT GGGACGCTGC GCCCGGCGCC
    GTCCGAAAGT ACATCATCAC TTACAAGCCA GAGGAGGGAG AGGCGAAGGA GCTTGAAGTT
    GACGGAGATG TAACATCTAG AGGCCTGGAT AATCTCATCT CCCAGACTGA ATATGATGTG
    GCTGTGACTC CGGTTTATGA TTCGGGCACT GGAAACCCCA TGCTTAACCA GGCCATTACA
    GATGTGGTTC CTGCCCCGCA GAACTTGAGG TTCTCGATGG TGACCCAGAC CAGTTTTAGG
    GTTCACTGGG AGCACGGCGC TCCTGATGTC TCCCTCTACC GTATTGGCTG GAAAAAGAAA
    GGAACGACCG AGTTTCAAAA TGATATCCTG AGTAGTGAAG ACACTACATA TGATCTGGTG
    GACCTGGAGC CAGACACACC TTATGATGTT TCGGTCACTG CCATTTACCC TGATGAGTCT
    GAGAGCGAGG ATCTGCTGGG CACTGAACGC ACTTTGGCCA AGCTTGCTAC ACCACCTCCC
    AGTGGTCCTC CTCAAAACCT CCAGGTGTTC GACGAGACCA CCACCTCCCT CACGACTAAG
    TGGGACCACG CGCCTGGACG AGTTCAGAAC TACAAAATCA CCTACGTACC GCTTACTGGA
    GGACGATCAC AGTCGACGCA GGTCGGAGGA AAGAAAAACA CAGTGATCCT GCAGAAACTG
    ACTCCAGACA CTCCTTACTC CATCACTGTT GCTGCGGTCT ACGCCAGCGG AGAGGCCAGA
    GACATTTCTG GCCAAGGAAA GACATTGCCG CTGGGCAGTG TCAGGAATCT TCAGGTCACT
    GACCCCACAG TTAGCACTCT GAATGTGCGC TGGGAGCCGG CGGAGGGAAG CGTTCGCCAG
    TACAGGATCT ACTACCAGCC CGTCGCAGGG GGCGCAGAGG ACATGGTCCA GGTTCCTGGA
    AGCACCACCA GCACCGTTCT GAAGAACCTG CAATCTGACA CTCAGTACAG AGTGACGGTG
    GTGCCAGTCT ACGCTCCTGG AGAGGGCAAG CGCATGTCGG AGACCGGAAA AACACTGGTT
    CGGACCCCTG CCAAGAACAT CCAGGTTTAC AACCCCACCA CAAACACCCT GAACGTGCGC
    TGGGAGCCGG CCTCAGGACA GGTGCAGCAG TACCGCGTGG CTTACGCCCC TCTCTCCGGG
    ATCCGACCCT CTCAGTCGGT GCTGGTTCCA GGAAACACCC ACACGGCTCT GCTGCAGCAG
    CTGGAATCAG ACACTGACTA CTCTGTGAGT GTGGTGGCTC TGTATGCTGA TGGAGAGGGC
    TCGTCTGTGT CTGATAATGG CAAAACACTG CCCCGCAGTG GCCCCAGGAA CCTGCGGGTT
    TATGATCCCA CCACCAGCAC GCTGTCGGTG GGCTGGGAAC ATGCAGAAGG GCCTGTGATT
    CAGTACCGCA TTGGCTACGC ACCCACCACT GGAGATCCCA TTGAAGAATT TACAACTGTC
    CCAGGCAACC GTAACTCGGT CATCCTGCAA AACCTGCAGC CAGACACGCC GTACAACATC
    AACGTAGAAG CCATTTATCA AGATGGCCCT GGAGGACGAC TCAGTGGAGA TGGAAAAACA
    TTGGATCTCC TTGGGCCTCG TAACCTGCGT GTTTCAGACG AGTGGTACAC ACGTTTCCGA
    GTGTCCTGGG ATCCTGCAGC CGCACGGGTG AATGGATATA AACTGGTCTA TACACCAGTG
    GGCACAACCG ACACCATGGA AGCCTTCGTT GGAGACGTGA CTTCACACCA CCTGAACAAC
    CTCCAGCCTG GCACCACATA CGACCTGAAG GTTTACGCTC AGTATGATGC GGGCTTGAGC
    GGCGCTCTGG TCGGACAGGG AACCACCCTG TATCTCAACG TGACCGACCT CACTTCCTAC
    AACGTGGGCT ACGACTCTTT CTGCATCAGA TGGACTCCTC ATCGAGCTGC CACCTCCTAC
    AGACTCAAAG TCAACCCTTT CAACCCATCT CACCGTGGAG CTCAGGAGGT CACGATCACT
    GCGGCTGAGA GCCAGTACTG TTTTGACGGC CTGACGCCAG ACGCGCTATA CAACGCCACC
    GTCTACACGC AGACCCCCAA CCGAGAGGGG CCCGGAGTCA GTGTCAAAGA GCGCACACTG
    GTGAAGCCCA CCGTCGCCCC AACACTACCT CCAACTCCTC CACCTCCAGC CACCATTCCT
    TCTGCACTCG AGGTGTGCAA AGGTGCCAAG GCTGACCTGG TGTTCCTGAT CGATGGCTCG
    TGGAGTATCG GAGATGACAG CTTCGCTAAA GTCCGGCAGT TTGTGTTCAG CATGATTGGG
    GCGTTTGATG TGGTCAGCCA TGAGGGCATG CAGGTGTCTT TCGTGCAGTA CAGCGACGAC
    GCCAAAACTG AGTTCAAGCT CAACACCTAC CACGACAAGG GAATGGTGCT GTCTGCTCTT
    CAGATGGTCA ATTATAGAGG AGGAAACACC AAAACAGGTG CTGCACTGAA GCATGTTGGA
    GAAAAAGTGT TCACCTCGGA CAATGGCATG AGGAGAACCG TTCCCAAGGT ACTGGTTGTG
    GTGACCGATG GACGTTCTCA AGATGACGTG AAGAAGAGCG CTGCTAAACT ACAGCATGCA
    GGTTACAGTG TGTTTGTGGT GGGTGTCGCT GACATTGACG TTAATGAACT GAGGAACATT
    GGAAGCAAGC CCAGTGACAG ACACGTCTTT ATCGTCGACA ACTTCGATGC TTTCGCCAAG
    ATCCAAGACA ACCTGATCAC GTTCATCTGT GAAACGGCAA CGTCCACATG CCCTTTGATT
    TACCTCAATG GATTCACATC ACCAGGCTTC AGGATGCTGG AAGCTTTCAA CATCACTGAC
    AAGACCTACG CAGGCATGAA CGGCGTCTCT CTGGAAACCG GCTCCTTCAA CAGCTTCATC
    GCATACAGAC TCCATAAGGA CGCCTTCATT TCCCAGCCAA CAAAGGAAAT CCATCCAGAT
    GGTCTTCCTC CATCCTACAC CCTCATCCTC CTCTTCCGTC TGCTCCCGGA CACCGGCAAG
    GATGCGTTTG ATTTATGGCA GATCTCAGAT AAGAACAATA ACCCTGAGGT CGGGCTCACC
    ATTGACCCCT CCAGCAGAGT GATCTCCTTC TACAATAAAG ACACTAGAGG GGAAATTCAG
    AAAGTCTCCT TTGATAACGA CCACGTGAAG AAGATTTTCC ACGGCAGTTT CCACAAGCTT
    CACATTTCTA TTTCGCCGAA AAATGTCAAA CTCAACATCG ACTGCCAAGA GGTGGCTGAG
    AAACAGATCA AAGCAGCCAA CAACGTCTCC ACAGATGGCT ACGAAGTCCT TGGAAAAATG
    ACAAAGTCTG GCGGTTCAAA GAAGCAGTCG GCCACATTCC AGCTCCAGAT GTTTGATATT
    GTCTGCAGTT TGGGCTGGAC AAGTCGAGAC AAATGTTGTG ATATTCCAGC AATGCGAGAT
    GAAGCCAAGT GTCCTGCTCT TCCTCACTCC TGCACATGTG CTCAGGACAG TGTTGGTCCT
    CCAGGCCCAG CGGGTCCTCA AGGCAGTCCT GGTACAAAAG GCCCACGAGG CGAGAGAGGA
    GAGTCTGGTC CTACGGGTCC AATGGGTCCA CGTGGAGAGC AGGGTCTGCC AGGGCAGATG
    GGGCCACCGG GTCCACAGGG CCCCAATGGA CTATCCCTTC CCGGAGAACC AGGGCGACCG
    GGACCTAAGG GAGACCCCGG TGACTCAGGT TTACCTGGAC AGAGAGGCCC GGTTGGCGCT
    GCAGGTCCAC TCGGCCCTGT TGGACCCGCT GGAGCAAGAG GTCCACCAGG AAGTGAAGGC
    CCATCTGGAC CAAGAGGTCC ATCGGGACCA ATGGGACCAC CGGGAGCTCC CGGAATGCCA
    GGAATCACCG GAAAACCAGG AAAACCCGGA GACACTGGCT TTCCTGGACC TGTTGGCATG
    AAAGGAGAGA AGGGTGAAAG AGGAGACACT GCGTCACAGA ATATGATGCG CTCTGTAGCG
    AGACAAGTCT GCGAACAGCT AGTCAGCAGA CAAATGAGCA GAATTGATAT GATGATGAAC
    CAGATCCCCA GCAACTACAG GAGTAACAGC CCCGGCCCCG CTGGACCTCC AGGCCCACCT
    GGAAACCAGG GACCCCATGG AGAGCCTGGT CAGCCCGGAC CCAATGGCTT CCCAGGAAAT
    CCAGGTTTAC CAGGCTCTCC TGGAGAAAGA GGAGTGCCTG GAGAGAAGGG TGAACGAGGC
    AGCCCCGGCA TTGGCACTCG AGGACAGAGA GGATTACCTG GACCTCCTGG GCCACCAGGG
    CAGTCTCAGA CCGGTCCCCC TGGTCCCTCA GGCTCACCCG GGCCCCGAGG ACCACCAGGA
    CAACATGGTT CTTCTGGAGT CACAGGACCA CCAGGACCTC CAGGATACTG TGACTCCTCT
    CAGTGCGGCA TGCCTTACAA CGGACAAGGC TACCCAGGCC CCTACCAGCC CGGGTCTGAA
    ACCTACGTAG TGCCCATACC AGCCGCGCCA TCGGAAGATA TCGAGACACA GTCGCCAGGA
    TTGCGCTACC AGCGCGGAAA AAGGTCGCTA TCAAGAAAGG AAGCCGAAAG AATCAAAACA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009291572.1
    CDS140..9202
    Translation

    Target ORF information:

    RefSeq Version XM_009293297.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio collagen, type XII, alpha 1a (col12a1a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009293297.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    ATGAAGATCA GGTTGTCTTT GGCCGCTGCT GCGCTCTTGA CTCTAGTACT CAGTTCAGTA 
    GAGGCTCAAG TGGAGCCTCC GTCAGACTTA AAATTTAAAA TCCTGAATGA AAACACAGTG
    CAAATGTCAT GGAGGAAGCC CTCGTCTCAG ATACAGGGCT ACAGAATACA GGTCACGTCA
    GATACAGATG ATTCTAAAGA ATTGAGTCTT CCTGCCTCGT CCACAAGCAC ATCAATTACA
    GATCTAACTC CAGATGTAGA CTATTCAGTG TCAATCAATT CATATGATGG TGCTGAAGAA
    AGTATCCCTA TCCTTGGACA AATCACAATT CAATCCAGCA ACACAAGTGG ACCAAGGAGG
    CCACAGTCAG AGGCTGTCAA GTGTTCAGTG AGTGCCGTTG CGGATCTGGT TTTCCTGGTG
    GACGGCTCTT GGAGTGTGGG AAGAGAAAAC TTTAGATTTA TCCGGAGTTT CATTGGGGCC
    ATGGCCGGGG CCTTCGACAT TGAAGAGGAT AAGACCAGAG TGGCTGTGGT TCAGTACAGC
    TCGGACACCA GGACAGAGTT CAGTCTAAAC ACACATTTCA GACGACCCGA TGTCCTGCGA
    GCCATTAAAA ATCTGCCGTA CAAGGGTGGA AATACCATGA CGGGTGATGC AATGGACTAT
    CTGGTGAAAA ACACGTTCAC TCAGGCGGCT GGAGCCAGGA AAGGCTTCCC AAAAGTGGCC
    ATGATCATCA CAGATGGAAA ATCTCAAGAC CCCGTAGAGG AGTACGCCGA ACGGTTGAGG
    AACATCGGAG TGGAGATCTT TGTGCTGGGT ATCAAAGGAG CAGATGAGGA CGAGCTGAAG
    GAGATCGCCT CCAGACCCCA TAGCAAGCAT GTCTATAACG TCCCCAACTT CGACATGATC
    AATCAGGTCC AGAAGGCGCT CATCACTGAA GTTTGCTCTG GCGTGGAGGA GCAGCTAAAT
    TCCCTTGTAA GCGGGGAGGA AGTTGTCGAA CCAGCTTCCA ACCTCAGAGT GACAGATATC
    TCCTCCAAAT CCATGAGAAT TGCGTGGGAT TCATCTCCTG GTGATATCAC AGGCTATAAG
    CTGCAGGTTT TTCCCACTAT TGCGAGCGCC AAAAAGCAGG AGCTCTACAC TGGGCCCACG
    ATGACCTTGG CCAATGTCAG AGACCTGACG CCTGAGATGG AGTATGAGAT CAGTCTGTTT
    GCTCTCAAAG GTCTGACTCC TAGTGTGCCG CAAGTTGTCA CTGAGAAAAC TCAGCCAGTG
    AAAGTATTTA CAGAGTGTTC CCTAGAGGTG GATGTCCAAG CTGACATTGT CCTCCTGGTG
    GATGGGTCAT ATAGTATAGG ACTGCCTAAC TTTGCGAAGG TTAGAGCTTT CCTCGAAGTA
    CTTGTGAATT CTTTTGACAT CGGACCTAAC AAAGTGCAGA TCAGCTTGGT TCAGTACAGC
    AGGGATCCTC ACACTGAGTT CGCCTTGAAT AAATTCGATG ACAATGCAGC CATGGTCAAG
    GCGGTCCGCA CCTTCCCCTA CCGAGGTGGA TCCACCAACA CCGGCAAGGC CATGACATAT
    GTGAGGGAAA AGATCTTTGT TTCGGGCCGT GGCGCTCGTG ATAACGTCCC CCGAGTCATG
    GTGCTTATCA CAGACGGCAA GTCCTCAGAT TCTTTCAAAG ACCCCGCCAA CAAACTGAGG
    GATACAGATG TGGAGATTTT TGCAGTGGGA GTAAAAGATG CCGTACGATC TGAGCTGGAG
    GCTATCGCCA ATGTTCCTGC TGATAACCAT GTGTTTGAGG TGGAGGACTT TGATGCTTTC
    GAGCGGATCT CCAAGGAGCT GACCGCGTCC ATCTGCCTCA GAATTGAGCA GGAGCTCCAT
    AACATCAGGA AAAGAAGTTT GCTTCCCCCA AAGTCACTGA GCTTCTCTGA GGTGACCTCC
    AGGAGTTTCA GGACCTCATG GGTTTCTGAT GGAGAGGATA TCCTGTCTTT TCTGGTGCGC
    TTCAGGCCAG CGGCGGACCT AACTGGAGAC TACATCTCTC TGGTGGTTCC CCATGACATC
    ACCAGTACAA TTCTGCCCAA TCTCACGCCA CTCACCACCT ACGAAGTTAA CGTCATTGCT
    CAGTATGATA AAGGAGACAG CTTTCCTTTG ACGGGCGAGG AGACCACTTT AGAAGAGTTG
    GGTTCTGTTC GTAACCTGAT GGTGAGCGAG GAGGCTGTGG ACCGCTTTCG GGTGTCGTGG
    AGAGCTGCTC CAGGATTAGT GCTGAGGTAC AGACTGCTCT ACGAGCCTGT GAGCGGAGGG
    GATAAACTGG AAGCAGAGAC TGATGGGAAT CAGGTCACCA CTGTGCTCAA TGAACTACTA
    CCGGTCACTA CTTACCGGGT CACCGTTTAC CCTGAGTACG CAAGTGGAAG GGGTCCCTCC
    ATGGACACTG AAGGCACCAC CAAAGAAGTT CGAGGTTCTC CACGCGACTT AAGGGTCTTC
    AATGAGACGG TGTCTAGCAT GAGGGTCTCG TGGCGGGCTG CCCCAGGCAA CGTGCTTCAA
    TATCAGGTGG CCTACAAACC TGACGGTGGG GAGAGGAAAG AAATAAGTGT TAAAGGAGAG
    ACAACAACTG CTGTGCTGAA AAATCTGCTG CCGGACACGG AGTATGAGCT GTTTGTTAGC
    GCACGTTACG TCTCTGGACT TGGCGCACCA CTTATGGGAA CAGGCACCAC GCTGGAAGAA
    CTTGGTTCAC CTTCGAATCT GGTAACTTCA GACGTCACTG AGAACAGTTT TGTAGCATCC
    TGGACCGCCG CCCCTGGAAA CGTCCGTCTT TATCGGGTCA CCTGGAAATC CCTCTTTTCA
    GAGGAAGCTG GCGAGAAGAC TGTCCCCGGG GACGTAACCA CCACTGTTCT GGACGGGCTG
    ACACCAGAGA CCCGCTACAA GGTTTCTGTC TACGCTGTCT ACAGCCGTGC GGAGGGGGCG
    CCACTGCACG GAGAGGAGAC CACAGATGTG TCGGCCGCTA CCAGAGCTCT TACGGTGTCA
    GAAGAAACAG AGAGGACCAT GAAGGTGACA TGGATACCAG CACCTGGCAA TGTTGTCAAT
    TACAGGGTAT CATACAAACC TCAGGGTGAT TTACGCCAAA TGGCCTCCAA AGCACCAGGA
    GGCACCAATT CTATAGTCCT GAGACGCCTG ATCCCCCAGA CCACTTACGA TATCACAGTG
    GTTCCCGTCT ACAAGTTTGG GGATGGAAGA CAAAGGCAAG GAGAAGGAAC TACATTGTCT
    CAATATAAAG CTCCCAGAAA CTTGCAAACT TCGGAGCCCA CTAAAACCAG TTTCAGGGTG
    ACCTGGGATC ATGCTCCTGG AGATGTAAAA GGCTACAAGG TCACTTTCCA TCCCACCGGA
    AAGGAAGTGG ACCTGGGAGA GTTACTCGTA GGCCCCTATG ACAACACTGT GGTTCTGGAG
    GAACTAAGGG CCGGCACTAA GTATACGGTA AATGTGTTTG GTATGTTTGA TGGAGGAGAG
    AGTATGCCAT TGGCTGGAGA GGAGAAGACC ACCCTCTCTG ACGCAGCTTC TGAAAACCTC
    CCTTATACAC CTTCAGATGC CCAGTGTAAA ACCACGGCCC AAGCGGATAT CGTCCTGCTT
    GTTGATGGAT CTTGGAGCAT TGGTCGCCTG AACTTCAAGA CCATACGCAA TTTCATTGCT
    CGCATGGTGG GAGTCTTCGA CATCGGCCCC GATCGTGTCC AGATTGGTCT GGCACAGTAC
    AGCGGAGACC CGAAGACTGA GTGGCATCTC AATGCTCACA GAACCAGGAC TCAGCTGCTG
    GATGCGGTGG CCAATCTACC CTACAAAGGA GGAAACACTC TCACCGGCCT TGCTTTGAAT
    TACATCCTTC AGAATAACTT CAAGCCTAAC GTTGGCATGC GTCCAAACTC CAGAAAGATC
    GGCGTCCTGG TCACTGATGG CAAGTCCCAA GACGACATTG TGGCGAACTC ACAGAACCTG
    CGGGACCAAG GGATTGAACT GTACGCCATT GGTGTAAAGA ACGCAGATGA GAATGAGTTG
    AGAACCATTG CTTCCGATCC CGATGACATT CACATGTACA ACGTGGCTGA TTTCTCCTTC
    CTGCTGGACA TTGTGGATGA CCTCACAAAC AACCTGTGCA ACAGCGTCAA AGGTCCAGGA
    GGAGGACCAG AGCCACCCAC TGATCTGACC ACCTCAGAGG TCACACATTA CTCTTTCCGT
    GCCACTTGGG TGGCTCCTGA CGGGCCTGTG GAGAAGTACA GAGTGGAGTA TGTGAGCACC
    GCTGGAGGAG AACCACAGCA ACAGATGTTT GTGGACGGGA CGGTGACCAC TGTGGTTTTG
    GAAGGTCTCA CTCCGCTGAC CGAGTACATA GTAAAGGTTT ACTCAGTGGT GGGAGAGGAC
    AGCAGCGACC CACTCAGAGG CATAGAGACC ACATTGCCTC TTCCTCCATC CGGTGAGTTG
    AGGATTACCG ATGTGACCCA CAGCACCATG CAGCTGGACT GGGACGCTGC GCCCGGCGCC
    GTCCGAAAGT ACATCATCAC TTACAAGCCA GAGGAGGGAG AGGCGAAGGA GCTTGAAGTT
    GACGGAGATG TAACATCTAG AGGCCTGGAT AATCTCATCT CCCAGACTGA ATATGATGTG
    GCTGTGACTC CGGTTTATGA TTCGGGCACT GGAAACCCCA TGCTTAACCA GGCCATTACA
    GATGTGGTTC CTGCCCCGCA GAACTTGAGG TTCTCGATGG TGACCCAGAC CAGTTTTAGG
    GTTCACTGGG AGCACGGCGC TCCTGATGTC TCCCTCTACC GTATTGGCTG GAAAAAGAAA
    GGAACGACCG AGTTTCAAAA TGATATCCTG AGTAGTGAAG ACACTACATA TGATCTGGTG
    GACCTGGAGC CAGACACACC TTATGATGTT TCGGTCACTG CCATTTACCC TGATGAGTCT
    GAGAGCGAGG ATCTGCTGGG CACTGAACGC ACTTTGGCCA AGCTTGCTAC ACCACCTCCC
    AGTGGTCCTC CTCAAAACCT CCAGGTGTTC GACGAGACCA CCACCTCCCT CACGACTAAG
    TGGGACCACG CGCCTGGACG AGTTCAGAAC TACAAAATCA CCTACGTACC GCTTACTGGA
    GGACGATCAC AGTCGACGCA GGTCGGAGGA AAGAAAAACA CAGTGATCCT GCAGAAACTG
    ACTCCAGACA CTCCTTACTC CATCACTGTT GCTGCGGTCT ACGCCAGCGG AGAGGCCAGA
    GACATTTCTG GCCAAGGAAA GACATTGCCG CTGGGCAGTG TCAGGAATCT TCAGGTCACT
    GACCCCACAG TTAGCACTCT GAATGTGCGC TGGGAGCCGG CGGAGGGAAG CGTTCGCCAG
    TACAGGATCT ACTACCAGCC CGTCGCAGGG GGCGCAGAGG ACATGGTCCA GGTTCCTGGA
    AGCACCACCA GCACCGTTCT GAAGAACCTG CAATCTGACA CTCAGTACAG AGTGACGGTG
    GTGCCAGTCT ACGCTCCTGG AGAGGGCAAG CGCATGTCGG AGACCGGAAA AACACTGGTT
    CGGACCCCTG CCAAGAACAT CCAGGTTTAC AACCCCACCA CAAACACCCT GAACGTGCGC
    TGGGAGCCGG CCTCAGGACA GGTGCAGCAG TACCGCGTGG CTTACGCCCC TCTCTCCGGG
    ATCCGACCCT CTCAGTCGGT GCTGGTTCCA GGAAACACCC ACACGGCTCT GCTGCAGCAG
    CTGGAATCAG ACACTGACTA CTCTGTGAGT GTGGTGGCTC TGTATGCTGA TGGAGAGGGC
    TCGTCTGTGT CTGATAATGG CAAAACACTG CCCCGCAGTG GCCCCAGGAA CCTGCGGGTT
    TATGATCCCA CCACCAGCAC GCTGTCGGTG GGCTGGGAAC ATGCAGAAGG GCCTGTGATT
    CAGTACCGCA TTGGCTACGC ACCCACCACT GGAGATCCCA TTGAAGAATT TACAACTGTC
    CCAGGCAACC GTAACTCGGT CATCCTGCAA AACCTGCAGC CAGACACGCC GTACAACATC
    AACGTAGAAG CCATTTATCA AGATGGCCCT GGAGGACGAC TCAGTGGAGA TGGAAAAACA
    TTGGATCTCC TTGGGCCTCG TAACCTGCGT GTTTCAGACG AGTGGTACAC ACGTTTCCGA
    GTGTCCTGGG ATCCTGCAGC CGCACGGGTG AATGGATATA AACTGGTCTA TACACCAGTG
    GGCACAACCG ACACCATGGA AGCCTTCGTT GGAGACGTGA CTTCACACCA CCTGAACAAC
    CTCCAGCCTG GCACCACATA CGACCTGAAG GTTTACGCTC AGTATGATGC GGGCTTGAGC
    GGCGCTCTGG TCGGACAGGG AACCACCCTG TATCTCAACG TGACCGACCT CACTTCCTAC
    AACGTGGGCT ACGACTCTTT CTGCATCAGA TGGACTCCTC ATCGAGCTGC CACCTCCTAC
    AGACTCAAAG TCAACCCTTT CAACCCATCT CACCGTGGAG CTCAGGAGGT CACGATCACT
    GCGGCTGAGA GCCAGTACTG TTTTGACGGC CTGACGCCAG ACGCGCTATA CAACGCCACC
    GTCTACACGC AGACCCCCAA CCGAGAGGGG CCCGGAGTCA GTGTCAAAGA GCGCACACTG
    GTGAAGCCCA CCGTCGCCCC AACACTACCT CCAACTCCTC CACCTCCAGC CACCATTCCT
    TCTGCACTCG AGGTGTGCAA AGGTGCCAAG GCTGACCTGG TGTTCCTGAT CGATGGCTCG
    TGGAGTATCG GAGATGACAG CTTCGCTAAA GTCCGGCAGT TTGTGTTCAG CATGATTGGG
    GCGTTTGATG TGGTCAGCCA TGAGGGCATG CAGGTGTCTT TCGTGCAGTA CAGCGACGAC
    GCCAAAACTG AGTTCAAGCT CAACACCTAC CACGACAAGG GAATGGTGCT GTCTGCTCTT
    CAGATGGTCA ATTATAGAGG AGGAAACACC AAAACAGGTG CTGCACTGAA GCATGTTGGA
    GAAAAAGTGT TCACCTCGGA CAATGGCATG AGGAGAACCG TTCCCAAGGT ACTGGTTGTG
    GTGACCGATG GACGTTCTCA AGATGACGTG AAGAAGAGCG CTGCTAAACT ACAGCATGCA
    GGTTACAGTG TGTTTGTGGT GGGTGTCGCT GACATTGACG TTAATGAACT GAGGAACATT
    GGAAGCAAGC CCAGTGACAG ACACGTCTTT ATCGTCGACA ACTTCGATGC TTTCGCCAAG
    ATCCAAGACA ACCTGATCAC GTTCATCTGT GAAACGGCAA CGTCCACATG CCCTTTGATT
    TACCTCAATG GATTCACATC ACCAGGCTTC AGGATGCTGG AAGCTTTCAA CATCACTGAC
    AAGACCTACG CAGGCATGAA CGGCGTCTCT CTGGAAACCG GCTCCTTCAA CAGCTTCATC
    GCATACAGAC TCCATAAGGA CGCCTTCATT TCCCAGCCAA CAAAGGAAAT CCATCCAGAT
    GGTCTTCCTC CATCCTACAC CCTCATCCTC CTCTTCCGTC TGCTCCCGGA CACCGGCAAG
    GATGCGTTTG ATTTATGGCA GATCTCAGAT AAGAACAATA ACCCTGAGGT CGGGCTCACC
    ATTGACCCCT CCAGCAGAGT GATCTCCTTC TACAATAAAG ACACTAGAGG GGAAATTCAG
    AAAGTCTCCT TTGATAACGA CCACGTGAAG AAGATTTTCC ACGGCAGTTT CCACAAGCTT
    CACATTTCTA TTTCGCCGAA AAATGTCAAA CTCAACATCG ACTGCCAAGA GGTGGCTGAG
    AAACAGATCA AAGCAGCCAA CAACGTCTCC ACAGATGGCT ACGAAGTCCT TGGAAAAATG
    ACAAAGTCTG GCGGTTCAAA GAAGCAGTCG GCCACATTCC AGCTCCAGAT GTTTGATATT
    GTCTGCAGTT TGGGCTGGAC AAGTCGAGAC AAATGTTGTG ATATTCCAGC AATGCGAGAT
    GAAGCCAAGT GTCCTGCTCT TCCTCACTCC TGCACATGTG CTCAGGACAG TGTTGGTCCT
    CCAGGCCCAG CGGGTCCTCA AGGCAGTCCT GGTACAAAAG GCCCACGAGG CGAGAGAGGA
    GAGTCTGGTC CTACGGGTCC AATGGGTCCA CGTGGAGAGC AGGGTCTGCC AGGGCAGATG
    GGGCCACCGG GTCCACAGGG CCCCAATGGA CTATCCCTTC CCGGAGAACC AGGGCGACCG
    GGACCTAAGG GAGACCCCGG TGACTCAGGT TTACCTGGAC AGAGAGGCCC GGTTGGCGCT
    GCAGGTCCAC TCGGCCCTGT TGGACCCGCT GGAGCAAGAG GTCCACCAGG AAGTGAAGGC
    CCATCTGGAC CAAGAGGTCC ATCGGGACCA ATGGGACCAC CGGGAGCTCC CGGAATGCCA
    GGAATCACCG GAAAACCAGG AAAACCCGGA GACACTGGCT TTCCTGGACC TGTTGGCATG
    AAAGGAGAGA AGGGTGAAAG AGGAGACACT GCGTCACAGA ATATGATGCG CTCTGTAGCG
    AGACAAGTCT GCGAACAGCT AGTCAGCAGA CAAATGAGCA GAATTGATAT GATGATGAAC
    CAGATCCCCA GCAACTACAG GAGTAACAGC CCCGGCCCCG CTGGACCTCC AGGCCCACCT
    GGAAACCAGG GACCCCATGG AGAGCCTGGT CAGCCCGGAC CCAATGGCTT CCCAGGAAAT
    CCAGGTTTAC CAGGCTCTCC TGGAGAAAGA GGAGTGCCTG GAGAGAAGGG TGAACGAGGC
    AGCCCCGGCA TTGGCACTCG AGGACAGAGA GGATTACCTG GACCTCCTGG GCCACCAGGG
    CAGTCTCAGA CCGGTCCCCC TGGTCCCTCA GGCTCACCCG GGCCCCGAGG ACCACCAGGA
    CAACATGGTT CTTCTGGAGT CACAGGACCA CCAGGACCTC CAGGATACTG TGACTCCTCT
    CAGTGCGGCA TGCCTTACAA CGGACAAGGC TACCCAGGCC CCTACCAGCC CGGGTCTGAA
    ACCTACGTAG TGCCCATACC AGCCGCGCCA TCGGAAGATA TCGAGACACA GTCGCCAGGA
    TTGCGCTACC AGCGCGGAAA AAGGTCGCTA TCAAGAAAGG AAGCCGAAAG AATCAAAACA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.