sec31b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	sec31b
Entrez Gene ID	100148383
Full Name	SEC31 homolog B, COPII coat complex component
General protein information	Preferred Names SEC31 homolog B, COPII coat complex component Names protein transport protein Sec31B protein transport protein Sec31A SEC31 homolog B, COPII coating complex component sec31l2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005173003.4	XP_005173060.1	protein transport protein Sec31A isoform X1
XM_001921546.7	XP_001921581.3	protein transport protein Sec31A isoform X3
XM_005173004.3	XP_005173061.1	protein transport protein Sec31A isoform X2
XM_005173003.3	XP_005173060.1	protein transport protein Sec31A isoform X1
XM_001921546.6	XP_001921581.3	protein transport protein Sec31A isoform X3
XM_005173004.4	XP_005173061.1	protein transport protein Sec31A isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SEC31B	XP_005637673.1
Rattus norvegicus (Norway rat)	Sec31b	NP_001129185.1
Saccharomyces cerevisiae (baker's yeast)	SEC31	NP_010086.2
Mus musculus (house mouse)	Sec31b	NP_001028515.1
Homo sapiens (human)	SEC31B	NP_056305.1
Gallus gallus (chicken)	SEC31B	XP_001232876.1
Danio rerio (zebrafish)	sec31b	XP_005173061.1
Kluyveromyces lactis	KLLA0F05159g	XP_455314.1
Eremothecium gossypii	AGOS_ADR090W	NP_984186.2
Arabidopsis thaliana (thale cress)	AT1G18830	NP_173317.2
Xenopus tropicalis (tropical clawed frog)	sec31b	XP_004915997.1
Schizosaccharomyces pombe (fission yeast)	sec31	NP_595582.1
Oryza sativa (rice)	Os07g0657200	NP_001060510.1
Pan troglodytes (chimpanzee)	SEC31B	XP_003951789.1
	SEC31B	XP_002805840.1
Bos taurus (cattle)	SEC31B	NP_001179090.1
Magnaporthe oryzae (rice blast fungus)	MGG_01108	XP_003717826.1
Neurospora crassa	NCU06738	XP_963510.1
Arabidopsis thaliana (thale cress)	AT3G63460	NP_851024.1

Gene Ontology

The following sec31b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sec31b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa18301	XM_005173003.4 Latest version!	Danio rerio SEC31 homolog B, COPII coat complex component (sec31b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa18301	XM_005173004.4 Latest version!	Danio rerio SEC31 homolog B, COPII coat complex component (sec31b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa18302	XM_001921546.7 Latest version!	Danio rerio SEC31 homolog B, COPII coat complex component (sec31b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
ODa18301	XM_005173003.3	Danio rerio SEC31 homolog B, COPII coat complex component (sec31b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa18302	XM_001921546.6	Danio rerio SEC31 homolog B, COPII coat complex component (sec31b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa18301	XM_005173004.3	Danio rerio SEC31 homolog B, COPII coat complex component (sec31b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sec31b ( XM_005173003.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005173060.1 Danio rerio SEC31 homolog B, COPII coat complex component (sec31b), transcript variant X1, mRNA.

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CloneID	ODa18301
Clone ID Related Accession (Same CDS sequence)	XM_005173003.4 , XM_005173004.3 , XM_005173003.3 , XM_005173004.4
Accession Version	XM_005173003.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein transport protein Sec31A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005173003.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGTGA AGGAGATCCT CAGGACGGCC ACACAGGCGT GGAGTCCTGC CAGACAACAC 
CCGGCCTACC TGGCCTTAGG CACCGCAGCG CAGCAGTTAG ACGCCTCCTT CAACACCAGC 
TCTGCCCTGG AGATCTTCGA TATGGATTTT TCAGATACGT CAATGGACAT GAAGCTCAGA 
GGAACTCTGG CCACCTCCAA CAGGTTTCAC AGTCTGATCT GGGTGGCGTT TGGTATGGAT 
GTCCAGCCGT CGTCGGGTCG TCTCATCGGA GGCAGTGAGA ATGGTAGCGT GACCGTCTTC 
AATCCCGACG TGATTCTGAC CGCAGGAGAG GATGCTGTGA TTGGCCAGTC TAATAAACAC 
ACGGGACCTG TTCGAGCCCT CGACTTCAAC CGCTTTCAGA GTAATCTGAT CGCATCAGGA 
GCCAATGACT CAGAGATCTA CATCTGGGAC CTGAACAACT TCAGCAATCC AATGACTCCC 
GGCACAAAGT CACAGCTCTG TGAGCCGGCG GAGGACGTGA GCGTGGTGGC CTGGAACAGT 
CAAGTGCAGC ACATTCTGGC CTCCGCAAAC CCCAGCGGGA AAGCAGTGGT GTGGGACCTG 
AGGAAGAACG AGCCCATCAT CAAAATCAGC GACCACAGCA ACAGGATGCA CTGCTCAGGG 
ATGCTGTGGC ATCCAGATGT GGCCACTCAG CTGGTTCTGG CCTCAGAGGA CGACAGGCTG 
CCAGTCATCC AGATGTGGGA TCTTCGGTTC GCAACATCAC CTCTAAAAGT GCTGGAGAAC 
CACACCAGAG GGATTTTGTC GATCTCATGG AGTCAAGCTG ATCCTGAGCT CCTGCTGAGT 
AGCGGGAAAG ACAACCGCAT CCTGTGCTGG AACCCCAACA CTGGAGAGGT GATTTATGAG 
CTGCCGACAG CCAATCAGTG GTGTTTCGAT GTGCAGTGGT GTCCTCGAAA CCCCGCCCTC 
CTGTCAGCCG CATCGTTTGA CGGACGCATC AGTGTTTACT CTGTTATGGG TGGACGACTG 
CAGCAGCAGC AGCACAGCAC AGTCGACCAG ATCTCCGCTT CGTTTGATAC TAATGACCCA 
TTTGGGATGG GTCAAACTCT GCCGCCTCTT CAGGTTCCTC AAGCCGCTGT GGAGACCACC 
ATTATTCCTC CGTTAAAAAA GCCTCCAAAG TGGGTGCGGA GGCCTGTAGG AGCATCTTTC 
GCTTTCGGTG GGAAACTGGT GACCTTTGAG AACCCTAACG TGGCTCCGCC CCAGCAACAA 
CAACCAGCCA ATCCACTCCC TCGACAGGTG CATGTGAGTC AGGTTACCAC AGAGACAGAG 
TTCCTCCAGC GCTCCACTGA GCTGCAGATG ACGCTGCAGT CCGGATCCTT CAGCAGCTTC 
TGCCACAGTA AAATCCAGAG CGCTCCGACC GACGCCGAGC AGGACATCTG GAGGTTCCTC 
ATGGTGAATT TTGAGGATGA AGCGCGTGTG AAGTTTTTGA GGCTTCTGGG ATTCAGTAAA 
GACGAGCTGC AGAGAAAGAT CTCCGCTTGT TTGGGAAGGA AACTTCAACC CAACGGTTTA 
GATGTGAATG ACCTTGCAGA GAAGATGCAG ACACTTTCAG ATCAGAGAGC AGATGAGTGC 
GCCGGAGCTG CTGACGGTTT GCCCGGTCGT GAATCTCCTG CTGATTTCTT CAGTCAGCTG 
CCGAAGAAAG AGAAAGACAA ACAGCCCTTC CAGATCCCCG TATCCTCAGA TACAGACGGT 
TTGATCAGTC AGGCGCTGTT AGTGGGAAAC TTCGAGGGCG CTGTGGATCT GTGTCTGAAT 
GACGGTCGGT TTGCGGAGGC CATCCTTCTG TCCATCAGCG GAGGAGAAGA ACTGCTGAAG 
AAAACCCAGC AGAGATACCT GCAGCGACAG ACCAACAGCA TCTCCATGCT GATCTCGTCA 
GTAGTGACAC AGAACTGGCG TGATATCGTT CAGAGCTGTG AGCTTGATAA CTGGAAGGAG 
GCGTTAGCAG CGTTGCTAAC CTACGCTAAC CCCGAGGAGT TTGCTAGTCT GTGCGATACT 
CTGGGCTCTC GTCTGGAGGC TGGCGGCTCA GAGAAGCTCT GTCTGCAGGC CTGTCTGTGC 
TTCATCTGCT CGGGGAACAT CGAGCGTCTG GTGGACTGCT GGACACTGCA GAGAGACTGC 
TCATCACCAC TCGCTCTCGA GGATCTGGTG GAGAAGGTGA TGATCCTGAG GAAGTCTATT 
GAGCGTCTGC GTAACATTGA GGTGTGTGTG CGCAGCTCAG CGCTGACCCA GAAGCTCATC 
CAGTACTCCA GTATTCTGGC ATCTCAGGGC AGCCTGAGCG CAGCACTGAA CTACCTGCCC 
AGTGACTCCG ACCAGGCTGC AGTGCGGATG TTGAGGGACA GACTGTCACA CGCCAAAGGA 
GAAATACAGC AGAAACCCTC AGCTCCAGCA GGAGGCGCTA AACCTGCACC AAACACACAC 
ACTCCTGCAG CACACACAAC GGCGCCATAC CCGTCCTCGG GGGCCCCTCA GATGCCACGT 
CTCTTCAGTC CTCAGACCGG CCCCGAACCT CCTGCTGGAG CAACAACACA CACACCCAGA 
CCAGCATACA CACAACACCC TGCTACAGCC CCAGGTTTTC ATCCGTTTCC TCCGGCCGCT 
CCTCAGGGTG CTCCTGGTGG TTTCTTCACT CCTCAGATGC CCTCCTCCAG CCTGGCGGGT 
CCCTCAATGC CGCCCTCGTC TCCTCCGGGC CCCATGATGC CCGCTGGTGT TCCCATGATG 
ATGCCCACTC CTCCAGCTCC AGGCTTCCTC TCGCCCACGT CTCTCCCCGG CGGGCCGAGG 
TTTCCAGGAT CTGCGCACAC ACAGGGCCCC GCTCCTCCTC CCACCGGGGC GTATCCGTCT 
CTGGGGGCCG CCGGGTATCC ACAGGGTGGC CCCGGAGCTC CTGCAGCCAA GAGCTTCAGC 
GGGGCCCCCG TCCCTCCGCC ACCCTCAGGG GCTCAGGATG GATGGAATGA CCCTCCGGCT 
GTGCGTGCAG GACAGCGCAA GAAAAAGCTG CCAGAGAACT ACACTCCTCC AGCACCGATT 
ACAGCTCCAG TGATGGGTTT CCCAGCGGAG GCTCCAGTGC TTCAGGATCG TGTGCAGGTG 
CCACCCGGAG CCCCTCAGGA GCCCAGCATA CAGTCCATAC AGCAGCTTCC TGCCGAGCGT 
GTGGAGCAGA AAGAAATCCC AGCCGAACAC ATGATCCTGA AGACCACCTT CAGCAGTCTG 
GTGCAGCGCT GTCAGCTGGC CGCCGCAGAC CCGCAAACGA AACGCAAGCT TGATGACGCC 
TCCAAACGTC TCGAGAATCT GTATGACAAA CTGCGAGATC AATCGCTTTC TCCCAGCATC 
CTCGGCGGTC TCCACGAGAT TAGCCGGTGT GTTAGCGCAC GCAGCTATGT GCAAGGTCTG 
GAGGTGCACA CGCAGGTGGT GAGCAGCAGC AACTTCAGCG AGATCTCGGC CTTCATGCCC 
GTCCTCAAAG TGCTCATGAC CATCGCCAAC AAGCTGGCCG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005173060.1
CDS	131..3655
Translation	MRVKEILRTATQAWSPARQHPAYLALGTAAQQLDASFNTSSALEIFDMDFSDTSMDMKLRGTLATSNRFHSLIWVAFGMDVQPSSGRLIGGSENGSVTVFNPDVILTAGEDAVIGQSNKHTGPVRALDFNRFQSNLIASGANDSEIYIWDLNNFSNPMTPGTKSQLCEPAEDVSVVAWNSQVQHILASANPSGKAVVWDLRKNEPIIKISDHSNRMHCSGMLWHPDVATQLVLASEDDRLPVIQMWDLRFATSPLKVLENHTRGILSISWSQADPELLLSSGKDNRILCWNPNTGEVIYELPTANQWCFDVQWCPRNPALLSAASFDGRISVYSVMGGRLQQQQHSTVDQISASFDTNDPFGMGQTLPPLQVPQAAVETTIIPPLKKPPKWVRRPVGASFAFGGKLVTFENPNVAPPQQQQPANPLPRQVHVSQVTTETEFLQRSTELQMTLQSGSFSSFCHSKIQSAPTDAEQDIWRFLMVNFEDEARVKFLRLLGFSKDELQRKISACLGRKLQPNGLDVNDLAEKMQTLSDQRADECAGAADGLPGRESPADFFSQLPKKEKDKQPFQIPVSSDTDGLISQALLVGNFEGAVDLCLNDGRFAEAILLSISGGEELLKKTQQRYLQRQTNSISMLISSVVTQNWRDIVQSCELDNWKEALAALLTYANPEEFASLCDTLGSRLEAGGSEKLCLQACLCFICSGNIERLVDCWTLQRDCSSPLALEDLVEKVMILRKSIERLRNIEVCVRSSALTQKLIQYSSILASQGSLSAALNYLPSDSDQAAVRMLRDRLSHAKGEIQQKPSAPAGGAKPAPNTHTPAAHTTAPYPSSGAPQMPRLFSPQTGPEPPAGATTHTPRPAYTQHPATAPGFHPFPPAAPQGAPGGFFTPQMPSSSLAGPSMPPSSPPGPMMPAGVPMMMPTPPAPGFLSPTSLPGGPRFPGSAHTQGPAPPPTGAYPSLGAAGYPQGGPGAPAAKSFSGAPVPPPPSGAQDGWNDPPAVRAGQRKKKLPENYTPPAPITAPVMGFPAEAPVLQDRVQVPPGAPQEPSIQSIQQLPAERVEQKEIPAEHMILKTTFSSLVQRCQLAAADPQTKRKLDDASKRLENLYDKLRDQSLSPSILGGLHEISRCVSARSYVQGLEVHTQVVSSSNFSEISAFMPVLKVLMTIANKLAV

Target ORF information:

RefSeq Version	XM_005173003.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SEC31 homolog B, COPII coat complex component (sec31b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005173003.4

ORF Insert Sequence:

ATGAGGGTGA AGGAGATCCT CAGGACGGCC ACACAGGCGT GGAGTCCTGC CAGACAACAC 
CCGGCCTACC TGGCCTTAGG CACCGCAGCG CAGCAGTTAG ACGCCTCCTT CAACACCAGC 
TCTGCCCTGG AGATCTTCGA TATGGATTTT TCAGATACGT CAATGGACAT GAAGCTCAGA 
GGAACTCTGG CCACCTCCAA CAGGTTTCAC AGTCTGATCT GGGTGGCGTT TGGTATGGAT 
GTCCAGCCGT CGTCGGGTCG TCTCATCGGA GGCAGTGAGA ATGGTAGCGT GACCGTCTTC 
AATCCCGACG TGATTCTGAC CGCAGGAGAG GATGCTGTGA TTGGCCAGTC TAATAAACAC 
ACGGGACCTG TTCGAGCCCT CGACTTCAAC CGCTTTCAGA GTAATCTGAT CGCATCAGGA 
GCCAATGACT CAGAGATCTA CATCTGGGAC CTGAACAACT TCAGCAATCC AATGACTCCC 
GGCACAAAGT CACAGCTCTG TGAGCCGGCG GAGGACGTGA GCGTGGTGGC CTGGAACAGT 
CAAGTGCAGC ACATTCTGGC CTCCGCAAAC CCCAGCGGGA AAGCAGTGGT GTGGGACCTG 
AGGAAGAACG AGCCCATCAT CAAAATCAGC GACCACAGCA ACAGGATGCA CTGCTCAGGG 
ATGCTGTGGC ATCCAGATGT GGCCACTCAG CTGGTTCTGG CCTCAGAGGA CGACAGGCTG 
CCAGTCATCC AGATGTGGGA TCTTCGGTTC GCAACATCAC CTCTAAAAGT GCTGGAGAAC 
CACACCAGAG GGATTTTGTC GATCTCATGG AGTCAAGCTG ATCCTGAGCT CCTGCTGAGT 
AGCGGGAAAG ACAACCGCAT CCTGTGCTGG AACCCCAACA CTGGAGAGGT GATTTATGAG 
CTGCCGACAG CCAATCAGTG GTGTTTCGAT GTGCAGTGGT GTCCTCGAAA CCCCGCCCTC 
CTGTCAGCCG CATCGTTTGA CGGACGCATC AGTGTTTACT CTGTTATGGG TGGACGACTG 
CAGCAGCAGC AGCACAGCAC AGTCGACCAG ATCTCCGCTT CGTTTGATAC TAATGACCCA 
TTTGGGATGG GTCAAACTCT GCCGCCTCTT CAGGTTCCTC AAGCCGCTGT GGAGACCACC 
ATTATTCCTC CGTTAAAAAA GCCTCCAAAG TGGGTGCGGA GGCCTGTAGG AGCATCTTTC 
GCTTTCGGTG GGAAACTGGT GACCTTTGAG AACCCTAACG TGGCTCCGCC CCAGCAACAA 
CAACCAGCCA ATCCACTCCC TCGACAGGTG CATGTGAGTC AGGTTACCAC AGAGACAGAG 
TTCCTCCAGC GCTCCACTGA GCTGCAGATG ACGCTGCAGT CCGGATCCTT CAGCAGCTTC 
TGCCACAGTA AAATCCAGAG CGCTCCGACC GACGCCGAGC AGGACATCTG GAGGTTCCTC 
ATGGTGAATT TTGAGGATGA AGCGCGTGTG AAGTTTTTGA GGCTTCTGGG ATTCAGTAAA 
GACGAGCTGC AGAGAAAGAT CTCCGCTTGT TTGGGAAGGA AACTTCAACC CAACGGTTTA 
GATGTGAATG ACCTTGCAGA GAAGATGCAG ACACTTTCAG ATCAGAGAGC AGATGAGTGC 
GCCGGAGCTG CTGACGGTTT GCCCGGTCGT GAATCTCCTG CTGATTTCTT CAGTCAGCTG 
CCGAAGAAAG AGAAAGACAA ACAGCCCTTC CAGATCCCCG TATCCTCAGA TACAGACGGT 
TTGATCAGTC AGGCGCTGTT AGTGGGAAAC TTCGAGGGCG CTGTGGATCT GTGTCTGAAT 
GACGGTCGGT TTGCGGAGGC CATCCTTCTG TCCATCAGCG GAGGAGAAGA ACTGCTGAAG 
AAAACCCAGC AGAGATACCT GCAGCGACAG ACCAACAGCA TCTCCATGCT GATCTCGTCA 
GTAGTGACAC AGAACTGGCG TGATATCGTT CAGAGCTGTG AGCTTGATAA CTGGAAGGAG 
GCGTTAGCAG CGTTGCTAAC CTACGCTAAC CCCGAGGAGT TTGCTAGTCT GTGCGATACT 
CTGGGCTCTC GTCTGGAGGC TGGCGGCTCA GAGAAGCTCT GTCTGCAGGC CTGTCTGTGC 
TTCATCTGCT CGGGGAACAT CGAGCGTCTG GTGGACTGCT GGACACTGCA GAGAGACTGC 
TCATCACCAC TCGCTCTCGA GGATCTGGTG GAGAAGGTGA TGATCCTGAG GAAGTCTATT 
GAGCGTCTGC GTAACATTGA GGTGTGTGTG CGCAGCTCAG CGCTGACCCA GAAGCTCATC 
CAGTACTCCA GTATTCTGGC ATCTCAGGGC AGCCTGAGCG CAGCACTGAA CTACCTGCCC 
AGTGACTCCG ACCAGGCTGC AGTGCGGATG TTGAGGGACA GACTGTCACA CGCCAAAGGA 
GAAATACAGC AGAAACCCTC AGCTCCAGCA GGAGGCGCTA AACCTGCACC AAACACACAC 
ACTCCTGCAG CACACACAAC GGCGCCATAC CCGTCCTCGG GGGCCCCTCA GATGCCACGT 
CTCTTCAGTC CTCAGACCGG CCCCGAACCT CCTGCTGGAG CAACAACACA CACACCCAGA 
CCAGCATACA CACAACACCC TGCTACAGCC CCAGGTTTTC ATCCGTTTCC TCCGGCCGCT 
CCTCAGGGTG CTCCTGGTGG TTTCTTCACT CCTCAGATGC CCTCCTCCAG CCTGGCGGGT 
CCCTCAATGC CGCCCTCGTC TCCTCCGGGC CCCATGATGC CCGCTGGTGT TCCCATGATG 
ATGCCCACTC CTCCAGCTCC AGGCTTCCTC TCGCCCACGT CTCTCCCCGG CGGGCCGAGG 
TTTCCAGGAT CTGCGCACAC ACAGGGCCCC GCTCCTCCTC CCACCGGGGC GTATCCGTCT 
CTGGGGGCCG CCGGGTATCC ACAGGGTGGC CCCGGAGCTC CTGCAGCCAA GAGCTTCAGC 
GGGGCCCCCG TCCCTCCGCC ACCCTCAGGG GCTCAGGATG GATGGAATGA CCCTCCGGCT 
GTGCGTGCAG GACAGCGCAA GAAAAAGCTG CCAGAGAACT ACACTCCTCC AGCACCGATT 
ACAGCTCCAG TGATGGGTTT CCCAGCGGAG GCTCCAGTGC TTCAGGATCG TGTGCAGGTG 
CCACCCGGAG CCCCTCAGGA GCCCAGCATA CAGTCCATAC AGCAGCTTCC TGCCGAGCGT 
GTGGAGCAGA AAGAAATCCC AGCCGAACAC ATGATCCTGA AGACCACCTT CAGCAGTCTG 
GTGCAGCGCT GTCAGCTGGC CGCCGCAGAC CCGCAAACGA AACGCAAGCT TGATGACGCC 
TCCAAACGTC TCGAGAATCT GTATGACAAA CTGCGAGATC AATCGCTTTC TCCCAGCATC 
CTCGGCGGTC TCCACGAGAT TAGCCGGTGT GTTAGCGCAC GCAGCTATGT GCAAGGTCTG 
GAGGTGCACA CGCAGGTGGT GAGCAGCAGC AACTTCAGCG AGATCTCGGC CTTCATGCCC 
GTCCTCAAAG TGCTCATGAC CATCGCCAAC AAGCTGGCCG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sec31b ( XM_005173004.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005173061.1 Danio rerio SEC31 homolog B, COPII coat complex component (sec31b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa18301
Clone ID Related Accession (Same CDS sequence)	XM_005173003.4 , XM_005173004.3 , XM_005173003.3 , XM_005173004.4
Accession Version	XM_005173004.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein transport protein Sec31A isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005173004.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGTGA AGGAGATCCT CAGGACGGCC ACACAGGCGT GGAGTCCTGC CAGACAACAC 
CCGGCCTACC TGGCCTTAGG CACCGCAGCG CAGCAGTTAG ACGCCTCCTT CAACACCAGC 
TCTGCCCTGG AGATCTTCGA TATGGATTTT TCAGATACGT CAATGGACAT GAAGCTCAGA 
GGAACTCTGG CCACCTCCAA CAGGTTTCAC AGTCTGATCT GGGTGGCGTT TGGTATGGAT 
GTCCAGCCGT CGTCGGGTCG TCTCATCGGA GGCAGTGAGA ATGGTAGCGT GACCGTCTTC 
AATCCCGACG TGATTCTGAC CGCAGGAGAG GATGCTGTGA TTGGCCAGTC TAATAAACAC 
ACGGGACCTG TTCGAGCCCT CGACTTCAAC CGCTTTCAGA GTAATCTGAT CGCATCAGGA 
GCCAATGACT CAGAGATCTA CATCTGGGAC CTGAACAACT TCAGCAATCC AATGACTCCC 
GGCACAAAGT CACAGCTCTG TGAGCCGGCG GAGGACGTGA GCGTGGTGGC CTGGAACAGT 
CAAGTGCAGC ACATTCTGGC CTCCGCAAAC CCCAGCGGGA AAGCAGTGGT GTGGGACCTG 
AGGAAGAACG AGCCCATCAT CAAAATCAGC GACCACAGCA ACAGGATGCA CTGCTCAGGG 
ATGCTGTGGC ATCCAGATGT GGCCACTCAG CTGGTTCTGG CCTCAGAGGA CGACAGGCTG 
CCAGTCATCC AGATGTGGGA TCTTCGGTTC GCAACATCAC CTCTAAAAGT GCTGGAGAAC 
CACACCAGAG GGATTTTGTC GATCTCATGG AGTCAAGCTG ATCCTGAGCT CCTGCTGAGT 
AGCGGGAAAG ACAACCGCAT CCTGTGCTGG AACCCCAACA CTGGAGAGGT GATTTATGAG 
CTGCCGACAG CCAATCAGTG GTGTTTCGAT GTGCAGTGGT GTCCTCGAAA CCCCGCCCTC 
CTGTCAGCCG CATCGTTTGA CGGACGCATC AGTGTTTACT CTGTTATGGG TGGACGACTG 
CAGCAGCAGC AGCACAGCAC AGTCGACCAG ATCTCCGCTT CGTTTGATAC TAATGACCCA 
TTTGGGATGG GTCAAACTCT GCCGCCTCTT CAGGTTCCTC AAGCCGCTGT GGAGACCACC 
ATTATTCCTC CGTTAAAAAA GCCTCCAAAG TGGGTGCGGA GGCCTGTAGG AGCATCTTTC 
GCTTTCGGTG GGAAACTGGT GACCTTTGAG AACCCTAACG TGGCTCCGCC CCAGCAACAA 
CAACCAGCCA ATCCACTCCC TCGACAGGTG CATGTGAGTC AGGTTACCAC AGAGACAGAG 
TTCCTCCAGC GCTCCACTGA GCTGCAGATG ACGCTGCAGT CCGGATCCTT CAGCAGCTTC 
TGCCACAGTA AAATCCAGAG CGCTCCGACC GACGCCGAGC AGGACATCTG GAGGTTCCTC 
ATGGTGAATT TTGAGGATGA AGCGCGTGTG AAGTTTTTGA GGCTTCTGGG ATTCAGTAAA 
GACGAGCTGC AGAGAAAGAT CTCCGCTTGT TTGGGAAGGA AACTTCAACC CAACGGTTTA 
GATGTGAATG ACCTTGCAGA GAAGATGCAG ACACTTTCAG ATCAGAGAGC AGATGAGTGC 
GCCGGAGCTG CTGACGGTTT GCCCGGTCGT GAATCTCCTG CTGATTTCTT CAGTCAGCTG 
CCGAAGAAAG AGAAAGACAA ACAGCCCTTC CAGATCCCCG TATCCTCAGA TACAGACGGT 
TTGATCAGTC AGGCGCTGTT AGTGGGAAAC TTCGAGGGCG CTGTGGATCT GTGTCTGAAT 
GACGGTCGGT TTGCGGAGGC CATCCTTCTG TCCATCAGCG GAGGAGAAGA ACTGCTGAAG 
AAAACCCAGC AGAGATACCT GCAGCGACAG ACCAACAGCA TCTCCATGCT GATCTCGTCA 
GTAGTGACAC AGAACTGGCG TGATATCGTT CAGAGCTGTG AGCTTGATAA CTGGAAGGAG 
GCGTTAGCAG CGTTGCTAAC CTACGCTAAC CCCGAGGAGT TTGCTAGTCT GTGCGATACT 
CTGGGCTCTC GTCTGGAGGC TGGCGGCTCA GAGAAGCTCT GTCTGCAGGC CTGTCTGTGC 
TTCATCTGCT CGGGGAACAT CGAGCGTCTG GTGGACTGCT GGACACTGCA GAGAGACTGC 
TCATCACCAC TCGCTCTCGA GGATCTGGTG GAGAAGGTGA TGATCCTGAG GAAGTCTATT 
GAGCGTCTGC GTAACATTGA GGTGTGTGTG CGCAGCTCAG CGCTGACCCA GAAGCTCATC 
CAGTACTCCA GTATTCTGGC ATCTCAGGGC AGCCTGAGCG CAGCACTGAA CTACCTGCCC 
AGTGACTCCG ACCAGGCTGC AGTGCGGATG TTGAGGGACA GACTGTCACA CGCCAAAGGA 
GAAATACAGC AGAAACCCTC AGCTCCAGCA GGAGGCGCTA AACCTGCACC AAACACACAC 
ACTCCTGCAG CACACACAAC GGCGCCATAC CCGTCCTCGG GGGCCCCTCA GATGCCACGT 
CTCTTCAGTC CTCAGACCGG CCCCGAACCT CCTGCTGGAG CAACAACACA CACACCCAGA 
CCAGCATACA CACAACACCC TGCTACAGCC CCAGGTTTTC ATCCGTTTCC TCCGGCCGCT 
CCTCAGGGTG CTCCTGGTGG TTTCTTCACT CCTCAGATGC CCTCCTCCAG CCTGGCGGGT 
CCCTCAATGC CGCCCTCGTC TCCTCCGGGC CCCATGATGC CCGCTGGTGT TCCCATGATG 
ATGCCCACTC CTCCAGCTCC AGGCTTCCTC TCGCCCACGT CTCTCCCCGG CGGGCCGAGG 
TTTCCAGGAT CTGCGCACAC ACAGGGCCCC GCTCCTCCTC CCACCGGGGC GTATCCGTCT 
CTGGGGGCCG CCGGGTATCC ACAGGGTGGC CCCGGAGCTC CTGCAGCCAA GAGCTTCAGC 
GGGGCCCCCG TCCCTCCGCC ACCCTCAGGG GCTCAGGATG GATGGAATGA CCCTCCGGCT 
GTGCGTGCAG GACAGCGCAA GAAAAAGCTG CCAGAGAACT ACACTCCTCC AGCACCGATT 
ACAGCTCCAG TGATGGGTTT CCCAGCGGAG GCTCCAGTGC TTCAGGATCG TGTGCAGGTG 
CCACCCGGAG CCCCTCAGGA GCCCAGCATA CAGTCCATAC AGCAGCTTCC TGCCGAGCGT 
GTGGAGCAGA AAGAAATCCC AGCCGAACAC ATGATCCTGA AGACCACCTT CAGCAGTCTG 
GTGCAGCGCT GTCAGCTGGC CGCCGCAGAC CCGCAAACGA AACGCAAGCT TGATGACGCC 
TCCAAACGTC TCGAGAATCT GTATGACAAA CTGCGAGATC AATCGCTTTC TCCCAGCATC 
CTCGGCGGTC TCCACGAGAT TAGCCGGTGT GTTAGCGCAC GCAGCTATGT GCAAGGTCTG 
GAGGTGCACA CGCAGGTGGT GAGCAGCAGC AACTTCAGCG AGATCTCGGC CTTCATGCCC 
GTCCTCAAAG TGCTCATGAC CATCGCCAAC AAGCTGGCCG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005173061.1
CDS	90..3614
Translation	MRVKEILRTATQAWSPARQHPAYLALGTAAQQLDASFNTSSALEIFDMDFSDTSMDMKLRGTLATSNRFHSLIWVAFGMDVQPSSGRLIGGSENGSVTVFNPDVILTAGEDAVIGQSNKHTGPVRALDFNRFQSNLIASGANDSEIYIWDLNNFSNPMTPGTKSQLCEPAEDVSVVAWNSQVQHILASANPSGKAVVWDLRKNEPIIKISDHSNRMHCSGMLWHPDVATQLVLASEDDRLPVIQMWDLRFATSPLKVLENHTRGILSISWSQADPELLLSSGKDNRILCWNPNTGEVIYELPTANQWCFDVQWCPRNPALLSAASFDGRISVYSVMGGRLQQQQHSTVDQISASFDTNDPFGMGQTLPPLQVPQAAVETTIIPPLKKPPKWVRRPVGASFAFGGKLVTFENPNVAPPQQQQPANPLPRQVHVSQVTTETEFLQRSTELQMTLQSGSFSSFCHSKIQSAPTDAEQDIWRFLMVNFEDEARVKFLRLLGFSKDELQRKISACLGRKLQPNGLDVNDLAEKMQTLSDQRADECAGAADGLPGRESPADFFSQLPKKEKDKQPFQIPVSSDTDGLISQALLVGNFEGAVDLCLNDGRFAEAILLSISGGEELLKKTQQRYLQRQTNSISMLISSVVTQNWRDIVQSCELDNWKEALAALLTYANPEEFASLCDTLGSRLEAGGSEKLCLQACLCFICSGNIERLVDCWTLQRDCSSPLALEDLVEKVMILRKSIERLRNIEVCVRSSALTQKLIQYSSILASQGSLSAALNYLPSDSDQAAVRMLRDRLSHAKGEIQQKPSAPAGGAKPAPNTHTPAAHTTAPYPSSGAPQMPRLFSPQTGPEPPAGATTHTPRPAYTQHPATAPGFHPFPPAAPQGAPGGFFTPQMPSSSLAGPSMPPSSPPGPMMPAGVPMMMPTPPAPGFLSPTSLPGGPRFPGSAHTQGPAPPPTGAYPSLGAAGYPQGGPGAPAAKSFSGAPVPPPPSGAQDGWNDPPAVRAGQRKKKLPENYTPPAPITAPVMGFPAEAPVLQDRVQVPPGAPQEPSIQSIQQLPAERVEQKEIPAEHMILKTTFSSLVQRCQLAAADPQTKRKLDDASKRLENLYDKLRDQSLSPSILGGLHEISRCVSARSYVQGLEVHTQVVSSSNFSEISAFMPVLKVLMTIANKLAV

Target ORF information:

RefSeq Version	XM_005173004.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio SEC31 homolog B, COPII coat complex component (sec31b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005173004.3

ORF Insert Sequence:

ATGAGGGTGA AGGAGATCCT CAGGACGGCC ACACAGGCGT GGAGTCCTGC CAGACAACAC 
CCGGCCTACC TGGCCTTAGG CACCGCAGCG CAGCAGTTAG ACGCCTCCTT CAACACCAGC 
TCTGCCCTGG AGATCTTCGA TATGGATTTT TCAGATACGT CAATGGACAT GAAGCTCAGA 
GGAACTCTGG CCACCTCCAA CAGGTTTCAC AGTCTGATCT GGGTGGCGTT TGGTATGGAT 
GTCCAGCCGT CGTCGGGTCG TCTCATCGGA GGCAGTGAGA ATGGTAGCGT GACCGTCTTC 
AATCCCGACG TGATTCTGAC CGCAGGAGAG GATGCTGTGA TTGGCCAGTC TAATAAACAC 
ACGGGACCTG TTCGAGCCCT CGACTTCAAC CGCTTTCAGA GTAATCTGAT CGCATCAGGA 
GCCAATGACT CAGAGATCTA CATCTGGGAC CTGAACAACT TCAGCAATCC AATGACTCCC 
GGCACAAAGT CACAGCTCTG TGAGCCGGCG GAGGACGTGA GCGTGGTGGC CTGGAACAGT 
CAAGTGCAGC ACATTCTGGC CTCCGCAAAC CCCAGCGGGA AAGCAGTGGT GTGGGACCTG 
AGGAAGAACG AGCCCATCAT CAAAATCAGC GACCACAGCA ACAGGATGCA CTGCTCAGGG 
ATGCTGTGGC ATCCAGATGT GGCCACTCAG CTGGTTCTGG CCTCAGAGGA CGACAGGCTG 
CCAGTCATCC AGATGTGGGA TCTTCGGTTC GCAACATCAC CTCTAAAAGT GCTGGAGAAC 
CACACCAGAG GGATTTTGTC GATCTCATGG AGTCAAGCTG ATCCTGAGCT CCTGCTGAGT 
AGCGGGAAAG ACAACCGCAT CCTGTGCTGG AACCCCAACA CTGGAGAGGT GATTTATGAG 
CTGCCGACAG CCAATCAGTG GTGTTTCGAT GTGCAGTGGT GTCCTCGAAA CCCCGCCCTC 
CTGTCAGCCG CATCGTTTGA CGGACGCATC AGTGTTTACT CTGTTATGGG TGGACGACTG 
CAGCAGCAGC AGCACAGCAC AGTCGACCAG ATCTCCGCTT CGTTTGATAC TAATGACCCA 
TTTGGGATGG GTCAAACTCT GCCGCCTCTT CAGGTTCCTC AAGCCGCTGT GGAGACCACC 
ATTATTCCTC CGTTAAAAAA GCCTCCAAAG TGGGTGCGGA GGCCTGTAGG AGCATCTTTC 
GCTTTCGGTG GGAAACTGGT GACCTTTGAG AACCCTAACG TGGCTCCGCC CCAGCAACAA 
CAACCAGCCA ATCCACTCCC TCGACAGGTG CATGTGAGTC AGGTTACCAC AGAGACAGAG 
TTCCTCCAGC GCTCCACTGA GCTGCAGATG ACGCTGCAGT CCGGATCCTT CAGCAGCTTC 
TGCCACAGTA AAATCCAGAG CGCTCCGACC GACGCCGAGC AGGACATCTG GAGGTTCCTC 
ATGGTGAATT TTGAGGATGA AGCGCGTGTG AAGTTTTTGA GGCTTCTGGG ATTCAGTAAA 
GACGAGCTGC AGAGAAAGAT CTCCGCTTGT TTGGGAAGGA AACTTCAACC CAACGGTTTA 
GATGTGAATG ACCTTGCAGA GAAGATGCAG ACACTTTCAG ATCAGAGAGC AGATGAGTGC 
GCCGGAGCTG CTGACGGTTT GCCCGGTCGT GAATCTCCTG CTGATTTCTT CAGTCAGCTG 
CCGAAGAAAG AGAAAGACAA ACAGCCCTTC CAGATCCCCG TATCCTCAGA TACAGACGGT 
TTGATCAGTC AGGCGCTGTT AGTGGGAAAC TTCGAGGGCG CTGTGGATCT GTGTCTGAAT 
GACGGTCGGT TTGCGGAGGC CATCCTTCTG TCCATCAGCG GAGGAGAAGA ACTGCTGAAG 
AAAACCCAGC AGAGATACCT GCAGCGACAG ACCAACAGCA TCTCCATGCT GATCTCGTCA 
GTAGTGACAC AGAACTGGCG TGATATCGTT CAGAGCTGTG AGCTTGATAA CTGGAAGGAG 
GCGTTAGCAG CGTTGCTAAC CTACGCTAAC CCCGAGGAGT TTGCTAGTCT GTGCGATACT 
CTGGGCTCTC GTCTGGAGGC TGGCGGCTCA GAGAAGCTCT GTCTGCAGGC CTGTCTGTGC 
TTCATCTGCT CGGGGAACAT CGAGCGTCTG GTGGACTGCT GGACACTGCA GAGAGACTGC 
TCATCACCAC TCGCTCTCGA GGATCTGGTG GAGAAGGTGA TGATCCTGAG GAAGTCTATT 
GAGCGTCTGC GTAACATTGA GGTGTGTGTG CGCAGCTCAG CGCTGACCCA GAAGCTCATC 
CAGTACTCCA GTATTCTGGC ATCTCAGGGC AGCCTGAGCG CAGCACTGAA CTACCTGCCC 
AGTGACTCCG ACCAGGCTGC AGTGCGGATG TTGAGGGACA GACTGTCACA CGCCAAAGGA 
GAAATACAGC AGAAACCCTC AGCTCCAGCA GGAGGCGCTA AACCTGCACC AAACACACAC 
ACTCCTGCAG CACACACAAC GGCGCCATAC CCGTCCTCGG GGGCCCCTCA GATGCCACGT 
CTCTTCAGTC CTCAGACCGG CCCCGAACCT CCTGCTGGAG CAACAACACA CACACCCAGA 
CCAGCATACA CACAACACCC TGCTACAGCC CCAGGTTTTC ATCCGTTTCC TCCGGCCGCT 
CCTCAGGGTG CTCCTGGTGG TTTCTTCACT CCTCAGATGC CCTCCTCCAG CCTGGCGGGT 
CCCTCAATGC CGCCCTCGTC TCCTCCGGGC CCCATGATGC CCGCTGGTGT TCCCATGATG 
ATGCCCACTC CTCCAGCTCC AGGCTTCCTC TCGCCCACGT CTCTCCCCGG CGGGCCGAGG 
TTTCCAGGAT CTGCGCACAC ACAGGGCCCC GCTCCTCCTC CCACCGGGGC GTATCCGTCT 
CTGGGGGCCG CCGGGTATCC ACAGGGTGGC CCCGGAGCTC CTGCAGCCAA GAGCTTCAGC 
GGGGCCCCCG TCCCTCCGCC ACCCTCAGGG GCTCAGGATG GATGGAATGA CCCTCCGGCT 
GTGCGTGCAG GACAGCGCAA GAAAAAGCTG CCAGAGAACT ACACTCCTCC AGCACCGATT 
ACAGCTCCAG TGATGGGTTT CCCAGCGGAG GCTCCAGTGC TTCAGGATCG TGTGCAGGTG 
CCACCCGGAG CCCCTCAGGA GCCCAGCATA CAGTCCATAC AGCAGCTTCC TGCCGAGCGT 
GTGGAGCAGA AAGAAATCCC AGCCGAACAC ATGATCCTGA AGACCACCTT CAGCAGTCTG 
GTGCAGCGCT GTCAGCTGGC CGCCGCAGAC CCGCAAACGA AACGCAAGCT TGATGACGCC 
TCCAAACGTC TCGAGAATCT GTATGACAAA CTGCGAGATC AATCGCTTTC TCCCAGCATC 
CTCGGCGGTC TCCACGAGAT TAGCCGGTGT GTTAGCGCAC GCAGCTATGT GCAAGGTCTG 
GAGGTGCACA CGCAGGTGGT GAGCAGCAGC AACTTCAGCG AGATCTCGGC CTTCATGCCC 
GTCCTCAAAG TGCTCATGAC CATCGCCAAC AAGCTGGCCG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sec31b ( XM_005173003.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005173060.1 Danio rerio SEC31 homolog B, COPII coat complex component (sec31b), transcript variant X1, mRNA.

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CloneID	ODa18301
Clone ID Related Accession (Same CDS sequence)	XM_005173003.4 , XM_005173004.3 , XM_005173003.3 , XM_005173004.4
Accession Version	XM_005173003.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein transport protein Sec31A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005173003.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGTGA AGGAGATCCT CAGGACGGCC ACACAGGCGT GGAGTCCTGC CAGACAACAC 
CCGGCCTACC TGGCCTTAGG CACCGCAGCG CAGCAGTTAG ACGCCTCCTT CAACACCAGC 
TCTGCCCTGG AGATCTTCGA TATGGATTTT TCAGATACGT CAATGGACAT GAAGCTCAGA 
GGAACTCTGG CCACCTCCAA CAGGTTTCAC AGTCTGATCT GGGTGGCGTT TGGTATGGAT 
GTCCAGCCGT CGTCGGGTCG TCTCATCGGA GGCAGTGAGA ATGGTAGCGT GACCGTCTTC 
AATCCCGACG TGATTCTGAC CGCAGGAGAG GATGCTGTGA TTGGCCAGTC TAATAAACAC 
ACGGGACCTG TTCGAGCCCT CGACTTCAAC CGCTTTCAGA GTAATCTGAT CGCATCAGGA 
GCCAATGACT CAGAGATCTA CATCTGGGAC CTGAACAACT TCAGCAATCC AATGACTCCC 
GGCACAAAGT CACAGCTCTG TGAGCCGGCG GAGGACGTGA GCGTGGTGGC CTGGAACAGT 
CAAGTGCAGC ACATTCTGGC CTCCGCAAAC CCCAGCGGGA AAGCAGTGGT GTGGGACCTG 
AGGAAGAACG AGCCCATCAT CAAAATCAGC GACCACAGCA ACAGGATGCA CTGCTCAGGG 
ATGCTGTGGC ATCCAGATGT GGCCACTCAG CTGGTTCTGG CCTCAGAGGA CGACAGGCTG 
CCAGTCATCC AGATGTGGGA TCTTCGGTTC GCAACATCAC CTCTAAAAGT GCTGGAGAAC 
CACACCAGAG GGATTTTGTC GATCTCATGG AGTCAAGCTG ATCCTGAGCT CCTGCTGAGT 
AGCGGGAAAG ACAACCGCAT CCTGTGCTGG AACCCCAACA CTGGAGAGGT GATTTATGAG 
CTGCCGACAG CCAATCAGTG GTGTTTCGAT GTGCAGTGGT GTCCTCGAAA CCCCGCCCTC 
CTGTCAGCCG CATCGTTTGA CGGACGCATC AGTGTTTACT CTGTTATGGG TGGACGACTG 
CAGCAGCAGC AGCACAGCAC AGTCGACCAG ATCTCCGCTT CGTTTGATAC TAATGACCCA 
TTTGGGATGG GTCAAACTCT GCCGCCTCTT CAGGTTCCTC AAGCCGCTGT GGAGACCACC 
ATTATTCCTC CGTTAAAAAA GCCTCCAAAG TGGGTGCGGA GGCCTGTAGG AGCATCTTTC 
GCTTTCGGTG GGAAACTGGT GACCTTTGAG AACCCTAACG TGGCTCCGCC CCAGCAACAA 
CAACCAGCCA ATCCACTCCC TCGACAGGTG CATGTGAGTC AGGTTACCAC AGAGACAGAG 
TTCCTCCAGC GCTCCACTGA GCTGCAGATG ACGCTGCAGT CCGGATCCTT CAGCAGCTTC 
TGCCACAGTA AAATCCAGAG CGCTCCGACC GACGCCGAGC AGGACATCTG GAGGTTCCTC 
ATGGTGAATT TTGAGGATGA AGCGCGTGTG AAGTTTTTGA GGCTTCTGGG ATTCAGTAAA 
GACGAGCTGC AGAGAAAGAT CTCCGCTTGT TTGGGAAGGA AACTTCAACC CAACGGTTTA 
GATGTGAATG ACCTTGCAGA GAAGATGCAG ACACTTTCAG ATCAGAGAGC AGATGAGTGC 
GCCGGAGCTG CTGACGGTTT GCCCGGTCGT GAATCTCCTG CTGATTTCTT CAGTCAGCTG 
CCGAAGAAAG AGAAAGACAA ACAGCCCTTC CAGATCCCCG TATCCTCAGA TACAGACGGT 
TTGATCAGTC AGGCGCTGTT AGTGGGAAAC TTCGAGGGCG CTGTGGATCT GTGTCTGAAT 
GACGGTCGGT TTGCGGAGGC CATCCTTCTG TCCATCAGCG GAGGAGAAGA ACTGCTGAAG 
AAAACCCAGC AGAGATACCT GCAGCGACAG ACCAACAGCA TCTCCATGCT GATCTCGTCA 
GTAGTGACAC AGAACTGGCG TGATATCGTT CAGAGCTGTG AGCTTGATAA CTGGAAGGAG 
GCGTTAGCAG CGTTGCTAAC CTACGCTAAC CCCGAGGAGT TTGCTAGTCT GTGCGATACT 
CTGGGCTCTC GTCTGGAGGC TGGCGGCTCA GAGAAGCTCT GTCTGCAGGC CTGTCTGTGC 
TTCATCTGCT CGGGGAACAT CGAGCGTCTG GTGGACTGCT GGACACTGCA GAGAGACTGC 
TCATCACCAC TCGCTCTCGA GGATCTGGTG GAGAAGGTGA TGATCCTGAG GAAGTCTATT 
GAGCGTCTGC GTAACATTGA GGTGTGTGTG CGCAGCTCAG CGCTGACCCA GAAGCTCATC 
CAGTACTCCA GTATTCTGGC ATCTCAGGGC AGCCTGAGCG CAGCACTGAA CTACCTGCCC 
AGTGACTCCG ACCAGGCTGC AGTGCGGATG TTGAGGGACA GACTGTCACA CGCCAAAGGA 
GAAATACAGC AGAAACCCTC AGCTCCAGCA GGAGGCGCTA AACCTGCACC AAACACACAC 
ACTCCTGCAG CACACACAAC GGCGCCATAC CCGTCCTCGG GGGCCCCTCA GATGCCACGT 
CTCTTCAGTC CTCAGACCGG CCCCGAACCT CCTGCTGGAG CAACAACACA CACACCCAGA 
CCAGCATACA CACAACACCC TGCTACAGCC CCAGGTTTTC ATCCGTTTCC TCCGGCCGCT 
CCTCAGGGTG CTCCTGGTGG TTTCTTCACT CCTCAGATGC CCTCCTCCAG CCTGGCGGGT 
CCCTCAATGC CGCCCTCGTC TCCTCCGGGC CCCATGATGC CCGCTGGTGT TCCCATGATG 
ATGCCCACTC CTCCAGCTCC AGGCTTCCTC TCGCCCACGT CTCTCCCCGG CGGGCCGAGG 
TTTCCAGGAT CTGCGCACAC ACAGGGCCCC GCTCCTCCTC CCACCGGGGC GTATCCGTCT 
CTGGGGGCCG CCGGGTATCC ACAGGGTGGC CCCGGAGCTC CTGCAGCCAA GAGCTTCAGC 
GGGGCCCCCG TCCCTCCGCC ACCCTCAGGG GCTCAGGATG GATGGAATGA CCCTCCGGCT 
GTGCGTGCAG GACAGCGCAA GAAAAAGCTG CCAGAGAACT ACACTCCTCC AGCACCGATT 
ACAGCTCCAG TGATGGGTTT CCCAGCGGAG GCTCCAGTGC TTCAGGATCG TGTGCAGGTG 
CCACCCGGAG CCCCTCAGGA GCCCAGCATA CAGTCCATAC AGCAGCTTCC TGCCGAGCGT 
GTGGAGCAGA AAGAAATCCC AGCCGAACAC ATGATCCTGA AGACCACCTT CAGCAGTCTG 
GTGCAGCGCT GTCAGCTGGC CGCCGCAGAC CCGCAAACGA AACGCAAGCT TGATGACGCC 
TCCAAACGTC TCGAGAATCT GTATGACAAA CTGCGAGATC AATCGCTTTC TCCCAGCATC 
CTCGGCGGTC TCCACGAGAT TAGCCGGTGT GTTAGCGCAC GCAGCTATGT GCAAGGTCTG 
GAGGTGCACA CGCAGGTGGT GAGCAGCAGC AACTTCAGCG AGATCTCGGC CTTCATGCCC 
GTCCTCAAAG TGCTCATGAC CATCGCCAAC AAGCTGGCCG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005173060.1
CDS	90..3614
Translation	MRVKEILRTATQAWSPARQHPAYLALGTAAQQLDASFNTSSALEIFDMDFSDTSMDMKLRGTLATSNRFHSLIWVAFGMDVQPSSGRLIGGSENGSVTVFNPDVILTAGEDAVIGQSNKHTGPVRALDFNRFQSNLIASGANDSEIYIWDLNNFSNPMTPGTKSQLCEPAEDVSVVAWNSQVQHILASANPSGKAVVWDLRKNEPIIKISDHSNRMHCSGMLWHPDVATQLVLASEDDRLPVIQMWDLRFATSPLKVLENHTRGILSISWSQADPELLLSSGKDNRILCWNPNTGEVIYELPTANQWCFDVQWCPRNPALLSAASFDGRISVYSVMGGRLQQQQHSTVDQISASFDTNDPFGMGQTLPPLQVPQAAVETTIIPPLKKPPKWVRRPVGASFAFGGKLVTFENPNVAPPQQQQPANPLPRQVHVSQVTTETEFLQRSTELQMTLQSGSFSSFCHSKIQSAPTDAEQDIWRFLMVNFEDEARVKFLRLLGFSKDELQRKISACLGRKLQPNGLDVNDLAEKMQTLSDQRADECAGAADGLPGRESPADFFSQLPKKEKDKQPFQIPVSSDTDGLISQALLVGNFEGAVDLCLNDGRFAEAILLSISGGEELLKKTQQRYLQRQTNSISMLISSVVTQNWRDIVQSCELDNWKEALAALLTYANPEEFASLCDTLGSRLEAGGSEKLCLQACLCFICSGNIERLVDCWTLQRDCSSPLALEDLVEKVMILRKSIERLRNIEVCVRSSALTQKLIQYSSILASQGSLSAALNYLPSDSDQAAVRMLRDRLSHAKGEIQQKPSAPAGGAKPAPNTHTPAAHTTAPYPSSGAPQMPRLFSPQTGPEPPAGATTHTPRPAYTQHPATAPGFHPFPPAAPQGAPGGFFTPQMPSSSLAGPSMPPSSPPGPMMPAGVPMMMPTPPAPGFLSPTSLPGGPRFPGSAHTQGPAPPPTGAYPSLGAAGYPQGGPGAPAAKSFSGAPVPPPPSGAQDGWNDPPAVRAGQRKKKLPENYTPPAPITAPVMGFPAEAPVLQDRVQVPPGAPQEPSIQSIQQLPAERVEQKEIPAEHMILKTTFSSLVQRCQLAAADPQTKRKLDDASKRLENLYDKLRDQSLSPSILGGLHEISRCVSARSYVQGLEVHTQVVSSSNFSEISAFMPVLKVLMTIANKLAV

Target ORF information:

RefSeq Version	XM_005173003.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio SEC31 homolog B, COPII coat complex component (sec31b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005173003.3

ORF Insert Sequence:

ATGAGGGTGA AGGAGATCCT CAGGACGGCC ACACAGGCGT GGAGTCCTGC CAGACAACAC 
CCGGCCTACC TGGCCTTAGG CACCGCAGCG CAGCAGTTAG ACGCCTCCTT CAACACCAGC 
TCTGCCCTGG AGATCTTCGA TATGGATTTT TCAGATACGT CAATGGACAT GAAGCTCAGA 
GGAACTCTGG CCACCTCCAA CAGGTTTCAC AGTCTGATCT GGGTGGCGTT TGGTATGGAT 
GTCCAGCCGT CGTCGGGTCG TCTCATCGGA GGCAGTGAGA ATGGTAGCGT GACCGTCTTC 
AATCCCGACG TGATTCTGAC CGCAGGAGAG GATGCTGTGA TTGGCCAGTC TAATAAACAC 
ACGGGACCTG TTCGAGCCCT CGACTTCAAC CGCTTTCAGA GTAATCTGAT CGCATCAGGA 
GCCAATGACT CAGAGATCTA CATCTGGGAC CTGAACAACT TCAGCAATCC AATGACTCCC 
GGCACAAAGT CACAGCTCTG TGAGCCGGCG GAGGACGTGA GCGTGGTGGC CTGGAACAGT 
CAAGTGCAGC ACATTCTGGC CTCCGCAAAC CCCAGCGGGA AAGCAGTGGT GTGGGACCTG 
AGGAAGAACG AGCCCATCAT CAAAATCAGC GACCACAGCA ACAGGATGCA CTGCTCAGGG 
ATGCTGTGGC ATCCAGATGT GGCCACTCAG CTGGTTCTGG CCTCAGAGGA CGACAGGCTG 
CCAGTCATCC AGATGTGGGA TCTTCGGTTC GCAACATCAC CTCTAAAAGT GCTGGAGAAC 
CACACCAGAG GGATTTTGTC GATCTCATGG AGTCAAGCTG ATCCTGAGCT CCTGCTGAGT 
AGCGGGAAAG ACAACCGCAT CCTGTGCTGG AACCCCAACA CTGGAGAGGT GATTTATGAG 
CTGCCGACAG CCAATCAGTG GTGTTTCGAT GTGCAGTGGT GTCCTCGAAA CCCCGCCCTC 
CTGTCAGCCG CATCGTTTGA CGGACGCATC AGTGTTTACT CTGTTATGGG TGGACGACTG 
CAGCAGCAGC AGCACAGCAC AGTCGACCAG ATCTCCGCTT CGTTTGATAC TAATGACCCA 
TTTGGGATGG GTCAAACTCT GCCGCCTCTT CAGGTTCCTC AAGCCGCTGT GGAGACCACC 
ATTATTCCTC CGTTAAAAAA GCCTCCAAAG TGGGTGCGGA GGCCTGTAGG AGCATCTTTC 
GCTTTCGGTG GGAAACTGGT GACCTTTGAG AACCCTAACG TGGCTCCGCC CCAGCAACAA 
CAACCAGCCA ATCCACTCCC TCGACAGGTG CATGTGAGTC AGGTTACCAC AGAGACAGAG 
TTCCTCCAGC GCTCCACTGA GCTGCAGATG ACGCTGCAGT CCGGATCCTT CAGCAGCTTC 
TGCCACAGTA AAATCCAGAG CGCTCCGACC GACGCCGAGC AGGACATCTG GAGGTTCCTC 
ATGGTGAATT TTGAGGATGA AGCGCGTGTG AAGTTTTTGA GGCTTCTGGG ATTCAGTAAA 
GACGAGCTGC AGAGAAAGAT CTCCGCTTGT TTGGGAAGGA AACTTCAACC CAACGGTTTA 
GATGTGAATG ACCTTGCAGA GAAGATGCAG ACACTTTCAG ATCAGAGAGC AGATGAGTGC 
GCCGGAGCTG CTGACGGTTT GCCCGGTCGT GAATCTCCTG CTGATTTCTT CAGTCAGCTG 
CCGAAGAAAG AGAAAGACAA ACAGCCCTTC CAGATCCCCG TATCCTCAGA TACAGACGGT 
TTGATCAGTC AGGCGCTGTT AGTGGGAAAC TTCGAGGGCG CTGTGGATCT GTGTCTGAAT 
GACGGTCGGT TTGCGGAGGC CATCCTTCTG TCCATCAGCG GAGGAGAAGA ACTGCTGAAG 
AAAACCCAGC AGAGATACCT GCAGCGACAG ACCAACAGCA TCTCCATGCT GATCTCGTCA 
GTAGTGACAC AGAACTGGCG TGATATCGTT CAGAGCTGTG AGCTTGATAA CTGGAAGGAG 
GCGTTAGCAG CGTTGCTAAC CTACGCTAAC CCCGAGGAGT TTGCTAGTCT GTGCGATACT 
CTGGGCTCTC GTCTGGAGGC TGGCGGCTCA GAGAAGCTCT GTCTGCAGGC CTGTCTGTGC 
TTCATCTGCT CGGGGAACAT CGAGCGTCTG GTGGACTGCT GGACACTGCA GAGAGACTGC 
TCATCACCAC TCGCTCTCGA GGATCTGGTG GAGAAGGTGA TGATCCTGAG GAAGTCTATT 
GAGCGTCTGC GTAACATTGA GGTGTGTGTG CGCAGCTCAG CGCTGACCCA GAAGCTCATC 
CAGTACTCCA GTATTCTGGC ATCTCAGGGC AGCCTGAGCG CAGCACTGAA CTACCTGCCC 
AGTGACTCCG ACCAGGCTGC AGTGCGGATG TTGAGGGACA GACTGTCACA CGCCAAAGGA 
GAAATACAGC AGAAACCCTC AGCTCCAGCA GGAGGCGCTA AACCTGCACC AAACACACAC 
ACTCCTGCAG CACACACAAC GGCGCCATAC CCGTCCTCGG GGGCCCCTCA GATGCCACGT 
CTCTTCAGTC CTCAGACCGG CCCCGAACCT CCTGCTGGAG CAACAACACA CACACCCAGA 
CCAGCATACA CACAACACCC TGCTACAGCC CCAGGTTTTC ATCCGTTTCC TCCGGCCGCT 
CCTCAGGGTG CTCCTGGTGG TTTCTTCACT CCTCAGATGC CCTCCTCCAG CCTGGCGGGT 
CCCTCAATGC CGCCCTCGTC TCCTCCGGGC CCCATGATGC CCGCTGGTGT TCCCATGATG 
ATGCCCACTC CTCCAGCTCC AGGCTTCCTC TCGCCCACGT CTCTCCCCGG CGGGCCGAGG 
TTTCCAGGAT CTGCGCACAC ACAGGGCCCC GCTCCTCCTC CCACCGGGGC GTATCCGTCT 
CTGGGGGCCG CCGGGTATCC ACAGGGTGGC CCCGGAGCTC CTGCAGCCAA GAGCTTCAGC 
GGGGCCCCCG TCCCTCCGCC ACCCTCAGGG GCTCAGGATG GATGGAATGA CCCTCCGGCT 
GTGCGTGCAG GACAGCGCAA GAAAAAGCTG CCAGAGAACT ACACTCCTCC AGCACCGATT 
ACAGCTCCAG TGATGGGTTT CCCAGCGGAG GCTCCAGTGC TTCAGGATCG TGTGCAGGTG 
CCACCCGGAG CCCCTCAGGA GCCCAGCATA CAGTCCATAC AGCAGCTTCC TGCCGAGCGT 
GTGGAGCAGA AAGAAATCCC AGCCGAACAC ATGATCCTGA AGACCACCTT CAGCAGTCTG 
GTGCAGCGCT GTCAGCTGGC CGCCGCAGAC CCGCAAACGA AACGCAAGCT TGATGACGCC 
TCCAAACGTC TCGAGAATCT GTATGACAAA CTGCGAGATC AATCGCTTTC TCCCAGCATC 
CTCGGCGGTC TCCACGAGAT TAGCCGGTGT GTTAGCGCAC GCAGCTATGT GCAAGGTCTG 
GAGGTGCACA CGCAGGTGGT GAGCAGCAGC AACTTCAGCG AGATCTCGGC CTTCATGCCC 
GTCCTCAAAG TGCTCATGAC CATCGCCAAC AAGCTGGCCG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sec31b ( XM_005173004.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005173061.1 Danio rerio SEC31 homolog B, COPII coat complex component (sec31b), transcript variant X2, mRNA.

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CloneID	ODa18301
Clone ID Related Accession (Same CDS sequence)	XM_005173003.4 , XM_005173004.3 , XM_005173003.3 , XM_005173004.4
Accession Version	XM_005173004.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein transport protein Sec31A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005173004.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGTGA AGGAGATCCT CAGGACGGCC ACACAGGCGT GGAGTCCTGC CAGACAACAC 
CCGGCCTACC TGGCCTTAGG CACCGCAGCG CAGCAGTTAG ACGCCTCCTT CAACACCAGC 
TCTGCCCTGG AGATCTTCGA TATGGATTTT TCAGATACGT CAATGGACAT GAAGCTCAGA 
GGAACTCTGG CCACCTCCAA CAGGTTTCAC AGTCTGATCT GGGTGGCGTT TGGTATGGAT 
GTCCAGCCGT CGTCGGGTCG TCTCATCGGA GGCAGTGAGA ATGGTAGCGT GACCGTCTTC 
AATCCCGACG TGATTCTGAC CGCAGGAGAG GATGCTGTGA TTGGCCAGTC TAATAAACAC 
ACGGGACCTG TTCGAGCCCT CGACTTCAAC CGCTTTCAGA GTAATCTGAT CGCATCAGGA 
GCCAATGACT CAGAGATCTA CATCTGGGAC CTGAACAACT TCAGCAATCC AATGACTCCC 
GGCACAAAGT CACAGCTCTG TGAGCCGGCG GAGGACGTGA GCGTGGTGGC CTGGAACAGT 
CAAGTGCAGC ACATTCTGGC CTCCGCAAAC CCCAGCGGGA AAGCAGTGGT GTGGGACCTG 
AGGAAGAACG AGCCCATCAT CAAAATCAGC GACCACAGCA ACAGGATGCA CTGCTCAGGG 
ATGCTGTGGC ATCCAGATGT GGCCACTCAG CTGGTTCTGG CCTCAGAGGA CGACAGGCTG 
CCAGTCATCC AGATGTGGGA TCTTCGGTTC GCAACATCAC CTCTAAAAGT GCTGGAGAAC 
CACACCAGAG GGATTTTGTC GATCTCATGG AGTCAAGCTG ATCCTGAGCT CCTGCTGAGT 
AGCGGGAAAG ACAACCGCAT CCTGTGCTGG AACCCCAACA CTGGAGAGGT GATTTATGAG 
CTGCCGACAG CCAATCAGTG GTGTTTCGAT GTGCAGTGGT GTCCTCGAAA CCCCGCCCTC 
CTGTCAGCCG CATCGTTTGA CGGACGCATC AGTGTTTACT CTGTTATGGG TGGACGACTG 
CAGCAGCAGC AGCACAGCAC AGTCGACCAG ATCTCCGCTT CGTTTGATAC TAATGACCCA 
TTTGGGATGG GTCAAACTCT GCCGCCTCTT CAGGTTCCTC AAGCCGCTGT GGAGACCACC 
ATTATTCCTC CGTTAAAAAA GCCTCCAAAG TGGGTGCGGA GGCCTGTAGG AGCATCTTTC 
GCTTTCGGTG GGAAACTGGT GACCTTTGAG AACCCTAACG TGGCTCCGCC CCAGCAACAA 
CAACCAGCCA ATCCACTCCC TCGACAGGTG CATGTGAGTC AGGTTACCAC AGAGACAGAG 
TTCCTCCAGC GCTCCACTGA GCTGCAGATG ACGCTGCAGT CCGGATCCTT CAGCAGCTTC 
TGCCACAGTA AAATCCAGAG CGCTCCGACC GACGCCGAGC AGGACATCTG GAGGTTCCTC 
ATGGTGAATT TTGAGGATGA AGCGCGTGTG AAGTTTTTGA GGCTTCTGGG ATTCAGTAAA 
GACGAGCTGC AGAGAAAGAT CTCCGCTTGT TTGGGAAGGA AACTTCAACC CAACGGTTTA 
GATGTGAATG ACCTTGCAGA GAAGATGCAG ACACTTTCAG ATCAGAGAGC AGATGAGTGC 
GCCGGAGCTG CTGACGGTTT GCCCGGTCGT GAATCTCCTG CTGATTTCTT CAGTCAGCTG 
CCGAAGAAAG AGAAAGACAA ACAGCCCTTC CAGATCCCCG TATCCTCAGA TACAGACGGT 
TTGATCAGTC AGGCGCTGTT AGTGGGAAAC TTCGAGGGCG CTGTGGATCT GTGTCTGAAT 
GACGGTCGGT TTGCGGAGGC CATCCTTCTG TCCATCAGCG GAGGAGAAGA ACTGCTGAAG 
AAAACCCAGC AGAGATACCT GCAGCGACAG ACCAACAGCA TCTCCATGCT GATCTCGTCA 
GTAGTGACAC AGAACTGGCG TGATATCGTT CAGAGCTGTG AGCTTGATAA CTGGAAGGAG 
GCGTTAGCAG CGTTGCTAAC CTACGCTAAC CCCGAGGAGT TTGCTAGTCT GTGCGATACT 
CTGGGCTCTC GTCTGGAGGC TGGCGGCTCA GAGAAGCTCT GTCTGCAGGC CTGTCTGTGC 
TTCATCTGCT CGGGGAACAT CGAGCGTCTG GTGGACTGCT GGACACTGCA GAGAGACTGC 
TCATCACCAC TCGCTCTCGA GGATCTGGTG GAGAAGGTGA TGATCCTGAG GAAGTCTATT 
GAGCGTCTGC GTAACATTGA GGTGTGTGTG CGCAGCTCAG CGCTGACCCA GAAGCTCATC 
CAGTACTCCA GTATTCTGGC ATCTCAGGGC AGCCTGAGCG CAGCACTGAA CTACCTGCCC 
AGTGACTCCG ACCAGGCTGC AGTGCGGATG TTGAGGGACA GACTGTCACA CGCCAAAGGA 
GAAATACAGC AGAAACCCTC AGCTCCAGCA GGAGGCGCTA AACCTGCACC AAACACACAC 
ACTCCTGCAG CACACACAAC GGCGCCATAC CCGTCCTCGG GGGCCCCTCA GATGCCACGT 
CTCTTCAGTC CTCAGACCGG CCCCGAACCT CCTGCTGGAG CAACAACACA CACACCCAGA 
CCAGCATACA CACAACACCC TGCTACAGCC CCAGGTTTTC ATCCGTTTCC TCCGGCCGCT 
CCTCAGGGTG CTCCTGGTGG TTTCTTCACT CCTCAGATGC CCTCCTCCAG CCTGGCGGGT 
CCCTCAATGC CGCCCTCGTC TCCTCCGGGC CCCATGATGC CCGCTGGTGT TCCCATGATG 
ATGCCCACTC CTCCAGCTCC AGGCTTCCTC TCGCCCACGT CTCTCCCCGG CGGGCCGAGG 
TTTCCAGGAT CTGCGCACAC ACAGGGCCCC GCTCCTCCTC CCACCGGGGC GTATCCGTCT 
CTGGGGGCCG CCGGGTATCC ACAGGGTGGC CCCGGAGCTC CTGCAGCCAA GAGCTTCAGC 
GGGGCCCCCG TCCCTCCGCC ACCCTCAGGG GCTCAGGATG GATGGAATGA CCCTCCGGCT 
GTGCGTGCAG GACAGCGCAA GAAAAAGCTG CCAGAGAACT ACACTCCTCC AGCACCGATT 
ACAGCTCCAG TGATGGGTTT CCCAGCGGAG GCTCCAGTGC TTCAGGATCG TGTGCAGGTG 
CCACCCGGAG CCCCTCAGGA GCCCAGCATA CAGTCCATAC AGCAGCTTCC TGCCGAGCGT 
GTGGAGCAGA AAGAAATCCC AGCCGAACAC ATGATCCTGA AGACCACCTT CAGCAGTCTG 
GTGCAGCGCT GTCAGCTGGC CGCCGCAGAC CCGCAAACGA AACGCAAGCT TGATGACGCC 
TCCAAACGTC TCGAGAATCT GTATGACAAA CTGCGAGATC AATCGCTTTC TCCCAGCATC 
CTCGGCGGTC TCCACGAGAT TAGCCGGTGT GTTAGCGCAC GCAGCTATGT GCAAGGTCTG 
GAGGTGCACA CGCAGGTGGT GAGCAGCAGC AACTTCAGCG AGATCTCGGC CTTCATGCCC 
GTCCTCAAAG TGCTCATGAC CATCGCCAAC AAGCTGGCCG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005173061.1
CDS	131..3655
Translation	MRVKEILRTATQAWSPARQHPAYLALGTAAQQLDASFNTSSALEIFDMDFSDTSMDMKLRGTLATSNRFHSLIWVAFGMDVQPSSGRLIGGSENGSVTVFNPDVILTAGEDAVIGQSNKHTGPVRALDFNRFQSNLIASGANDSEIYIWDLNNFSNPMTPGTKSQLCEPAEDVSVVAWNSQVQHILASANPSGKAVVWDLRKNEPIIKISDHSNRMHCSGMLWHPDVATQLVLASEDDRLPVIQMWDLRFATSPLKVLENHTRGILSISWSQADPELLLSSGKDNRILCWNPNTGEVIYELPTANQWCFDVQWCPRNPALLSAASFDGRISVYSVMGGRLQQQQHSTVDQISASFDTNDPFGMGQTLPPLQVPQAAVETTIIPPLKKPPKWVRRPVGASFAFGGKLVTFENPNVAPPQQQQPANPLPRQVHVSQVTTETEFLQRSTELQMTLQSGSFSSFCHSKIQSAPTDAEQDIWRFLMVNFEDEARVKFLRLLGFSKDELQRKISACLGRKLQPNGLDVNDLAEKMQTLSDQRADECAGAADGLPGRESPADFFSQLPKKEKDKQPFQIPVSSDTDGLISQALLVGNFEGAVDLCLNDGRFAEAILLSISGGEELLKKTQQRYLQRQTNSISMLISSVVTQNWRDIVQSCELDNWKEALAALLTYANPEEFASLCDTLGSRLEAGGSEKLCLQACLCFICSGNIERLVDCWTLQRDCSSPLALEDLVEKVMILRKSIERLRNIEVCVRSSALTQKLIQYSSILASQGSLSAALNYLPSDSDQAAVRMLRDRLSHAKGEIQQKPSAPAGGAKPAPNTHTPAAHTTAPYPSSGAPQMPRLFSPQTGPEPPAGATTHTPRPAYTQHPATAPGFHPFPPAAPQGAPGGFFTPQMPSSSLAGPSMPPSSPPGPMMPAGVPMMMPTPPAPGFLSPTSLPGGPRFPGSAHTQGPAPPPTGAYPSLGAAGYPQGGPGAPAAKSFSGAPVPPPPSGAQDGWNDPPAVRAGQRKKKLPENYTPPAPITAPVMGFPAEAPVLQDRVQVPPGAPQEPSIQSIQQLPAERVEQKEIPAEHMILKTTFSSLVQRCQLAAADPQTKRKLDDASKRLENLYDKLRDQSLSPSILGGLHEISRCVSARSYVQGLEVHTQVVSSSNFSEISAFMPVLKVLMTIANKLAV

Target ORF information:

RefSeq Version	XM_005173004.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SEC31 homolog B, COPII coat complex component (sec31b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005173004.4

ORF Insert Sequence:

ATGAGGGTGA AGGAGATCCT CAGGACGGCC ACACAGGCGT GGAGTCCTGC CAGACAACAC 
CCGGCCTACC TGGCCTTAGG CACCGCAGCG CAGCAGTTAG ACGCCTCCTT CAACACCAGC 
TCTGCCCTGG AGATCTTCGA TATGGATTTT TCAGATACGT CAATGGACAT GAAGCTCAGA 
GGAACTCTGG CCACCTCCAA CAGGTTTCAC AGTCTGATCT GGGTGGCGTT TGGTATGGAT 
GTCCAGCCGT CGTCGGGTCG TCTCATCGGA GGCAGTGAGA ATGGTAGCGT GACCGTCTTC 
AATCCCGACG TGATTCTGAC CGCAGGAGAG GATGCTGTGA TTGGCCAGTC TAATAAACAC 
ACGGGACCTG TTCGAGCCCT CGACTTCAAC CGCTTTCAGA GTAATCTGAT CGCATCAGGA 
GCCAATGACT CAGAGATCTA CATCTGGGAC CTGAACAACT TCAGCAATCC AATGACTCCC 
GGCACAAAGT CACAGCTCTG TGAGCCGGCG GAGGACGTGA GCGTGGTGGC CTGGAACAGT 
CAAGTGCAGC ACATTCTGGC CTCCGCAAAC CCCAGCGGGA AAGCAGTGGT GTGGGACCTG 
AGGAAGAACG AGCCCATCAT CAAAATCAGC GACCACAGCA ACAGGATGCA CTGCTCAGGG 
ATGCTGTGGC ATCCAGATGT GGCCACTCAG CTGGTTCTGG CCTCAGAGGA CGACAGGCTG 
CCAGTCATCC AGATGTGGGA TCTTCGGTTC GCAACATCAC CTCTAAAAGT GCTGGAGAAC 
CACACCAGAG GGATTTTGTC GATCTCATGG AGTCAAGCTG ATCCTGAGCT CCTGCTGAGT 
AGCGGGAAAG ACAACCGCAT CCTGTGCTGG AACCCCAACA CTGGAGAGGT GATTTATGAG 
CTGCCGACAG CCAATCAGTG GTGTTTCGAT GTGCAGTGGT GTCCTCGAAA CCCCGCCCTC 
CTGTCAGCCG CATCGTTTGA CGGACGCATC AGTGTTTACT CTGTTATGGG TGGACGACTG 
CAGCAGCAGC AGCACAGCAC AGTCGACCAG ATCTCCGCTT CGTTTGATAC TAATGACCCA 
TTTGGGATGG GTCAAACTCT GCCGCCTCTT CAGGTTCCTC AAGCCGCTGT GGAGACCACC 
ATTATTCCTC CGTTAAAAAA GCCTCCAAAG TGGGTGCGGA GGCCTGTAGG AGCATCTTTC 
GCTTTCGGTG GGAAACTGGT GACCTTTGAG AACCCTAACG TGGCTCCGCC CCAGCAACAA 
CAACCAGCCA ATCCACTCCC TCGACAGGTG CATGTGAGTC AGGTTACCAC AGAGACAGAG 
TTCCTCCAGC GCTCCACTGA GCTGCAGATG ACGCTGCAGT CCGGATCCTT CAGCAGCTTC 
TGCCACAGTA AAATCCAGAG CGCTCCGACC GACGCCGAGC AGGACATCTG GAGGTTCCTC 
ATGGTGAATT TTGAGGATGA AGCGCGTGTG AAGTTTTTGA GGCTTCTGGG ATTCAGTAAA 
GACGAGCTGC AGAGAAAGAT CTCCGCTTGT TTGGGAAGGA AACTTCAACC CAACGGTTTA 
GATGTGAATG ACCTTGCAGA GAAGATGCAG ACACTTTCAG ATCAGAGAGC AGATGAGTGC 
GCCGGAGCTG CTGACGGTTT GCCCGGTCGT GAATCTCCTG CTGATTTCTT CAGTCAGCTG 
CCGAAGAAAG AGAAAGACAA ACAGCCCTTC CAGATCCCCG TATCCTCAGA TACAGACGGT 
TTGATCAGTC AGGCGCTGTT AGTGGGAAAC TTCGAGGGCG CTGTGGATCT GTGTCTGAAT 
GACGGTCGGT TTGCGGAGGC CATCCTTCTG TCCATCAGCG GAGGAGAAGA ACTGCTGAAG 
AAAACCCAGC AGAGATACCT GCAGCGACAG ACCAACAGCA TCTCCATGCT GATCTCGTCA 
GTAGTGACAC AGAACTGGCG TGATATCGTT CAGAGCTGTG AGCTTGATAA CTGGAAGGAG 
GCGTTAGCAG CGTTGCTAAC CTACGCTAAC CCCGAGGAGT TTGCTAGTCT GTGCGATACT 
CTGGGCTCTC GTCTGGAGGC TGGCGGCTCA GAGAAGCTCT GTCTGCAGGC CTGTCTGTGC 
TTCATCTGCT CGGGGAACAT CGAGCGTCTG GTGGACTGCT GGACACTGCA GAGAGACTGC 
TCATCACCAC TCGCTCTCGA GGATCTGGTG GAGAAGGTGA TGATCCTGAG GAAGTCTATT 
GAGCGTCTGC GTAACATTGA GGTGTGTGTG CGCAGCTCAG CGCTGACCCA GAAGCTCATC 
CAGTACTCCA GTATTCTGGC ATCTCAGGGC AGCCTGAGCG CAGCACTGAA CTACCTGCCC 
AGTGACTCCG ACCAGGCTGC AGTGCGGATG TTGAGGGACA GACTGTCACA CGCCAAAGGA 
GAAATACAGC AGAAACCCTC AGCTCCAGCA GGAGGCGCTA AACCTGCACC AAACACACAC 
ACTCCTGCAG CACACACAAC GGCGCCATAC CCGTCCTCGG GGGCCCCTCA GATGCCACGT 
CTCTTCAGTC CTCAGACCGG CCCCGAACCT CCTGCTGGAG CAACAACACA CACACCCAGA 
CCAGCATACA CACAACACCC TGCTACAGCC CCAGGTTTTC ATCCGTTTCC TCCGGCCGCT 
CCTCAGGGTG CTCCTGGTGG TTTCTTCACT CCTCAGATGC CCTCCTCCAG CCTGGCGGGT 
CCCTCAATGC CGCCCTCGTC TCCTCCGGGC CCCATGATGC CCGCTGGTGT TCCCATGATG 
ATGCCCACTC CTCCAGCTCC AGGCTTCCTC TCGCCCACGT CTCTCCCCGG CGGGCCGAGG 
TTTCCAGGAT CTGCGCACAC ACAGGGCCCC GCTCCTCCTC CCACCGGGGC GTATCCGTCT 
CTGGGGGCCG CCGGGTATCC ACAGGGTGGC CCCGGAGCTC CTGCAGCCAA GAGCTTCAGC 
GGGGCCCCCG TCCCTCCGCC ACCCTCAGGG GCTCAGGATG GATGGAATGA CCCTCCGGCT 
GTGCGTGCAG GACAGCGCAA GAAAAAGCTG CCAGAGAACT ACACTCCTCC AGCACCGATT 
ACAGCTCCAG TGATGGGTTT CCCAGCGGAG GCTCCAGTGC TTCAGGATCG TGTGCAGGTG 
CCACCCGGAG CCCCTCAGGA GCCCAGCATA CAGTCCATAC AGCAGCTTCC TGCCGAGCGT 
GTGGAGCAGA AAGAAATCCC AGCCGAACAC ATGATCCTGA AGACCACCTT CAGCAGTCTG 
GTGCAGCGCT GTCAGCTGGC CGCCGCAGAC CCGCAAACGA AACGCAAGCT TGATGACGCC 
TCCAAACGTC TCGAGAATCT GTATGACAAA CTGCGAGATC AATCGCTTTC TCCCAGCATC 
CTCGGCGGTC TCCACGAGAT TAGCCGGTGT GTTAGCGCAC GCAGCTATGT GCAAGGTCTG 
GAGGTGCACA CGCAGGTGGT GAGCAGCAGC AACTTCAGCG AGATCTCGGC CTTCATGCCC 
GTCCTCAAAG TGCTCATGAC CATCGCCAAC AAGCTGGCCG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sec31b ( XM_001921546.7 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001921581.3 Danio rerio SEC31 homolog B, COPII coat complex component (sec31b), transcript variant X3, mRNA.

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CloneID	ODa18302
Clone ID Related Accession (Same CDS sequence)	XM_001921546.7 , XM_001921546.6
Accession Version	XM_001921546.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein transport protein Sec31A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001921546.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGTGA AGGAGATCCT CAGGACGGCC ACACAGGCGT GGAGTCCTGC CAGACAACAC 
CCGGCCTACC TGGCCTTAGG CACCGCAGCG CAGCAGTTAG ACGCCTCCTT CAACACCAGC 
TCTGCCCTGG AGATCTTCGA TATGGATTTT TCAGATACGT CAATGGACAT GAAGCTCAGA 
GGAACTCTGG CCACCTCCAA CAGGTTTCAC AGTCTGATCT GGGTGGCGTT TGGTATGGAT 
GTCCAGCCGT CGTCGGGTCG TCTCATCGGA GGCAGTGAGA ATGGTAGCGT GACCGTCTTC 
AATCCCGACG TGATTCTGAC CGCAGGAGAG GATGCTGTGA TTGGCCAGTC TAATAAACAC 
ACGGGACCTG TTCGAGCCCT CGACTTCAAC CGCTTTCAGA GTAATCTGAT CGCATCAGGA 
GCCAATGACT CAGAGATCTA CATCTGGGAC CTGAACAACT TCAGCAATCC AATGACTCCC 
GGCACAAAGT CACAGCCGGC GGAGGACGTG AGCGTGGTGG CCTGGAACAG TCAAGTGCAG 
CACATTCTGG CCTCCGCAAA CCCCAGCGGG AAAGCAGTGG TGTGGGACCT GAGGAAGAAC 
GAGCCCATCA TCAAAATCAG CGACCACAGC AACAGGATGC ACTGCTCAGG GATGCTGTGG 
CATCCAGATG TGGCCACTCA GCTGGTTCTG GCCTCAGAGG ACGACAGGCT GCCAGTCATC 
CAGATGTGGG ATCTTCGGTT CGCAACATCA CCTCTAAAAG TGCTGGAGAA CCACACCAGA 
GGGATTTTGT CGATCTCATG GAGTCAAGCT GATCCTGAGC TCCTGCTGAG TAGCGGGAAA 
GACAACCGCA TCCTGTGCTG GAACCCCAAC ACTGGAGAGG TGATTTATGA GCTGCCGACA 
GCCAATCAGT GGTGTTTCGA TGTGCAGTGG TGTCCTCGAA ACCCCGCCCT CCTGTCAGCC 
GCATCGTTTG ACGGACGCAT CAGTGTTTAC TCTGTTATGG GTGGACGACT GCAGCAGCAG 
CAGCACAGCA CAGTCGACCA GATCTCCGCT TCGTTTGATA CTAATGACCC ATTTGGGATG 
GGTCAAACTC TGCCGCCTCT TCAGGTTCCT CAAGCCGCTG TGGAGACCAC CATTATTCCT 
CCGTTAAAAA AGCCTCCAAA GTGGGTGCGG AGGCCTGTAG GAGCATCTTT CGCTTTCGGT 
GGGAAACTGG TGACCTTTGA GAACCCTAAC GTGGCTCCGC CCCAGCAACA ACAACCAGCC 
AATCCACTCC CTCGACAGGT GCATGTGAGT CAGGTTACCA CAGAGACAGA GTTCCTCCAG 
CGCTCCACTG AGCTGCAGAT GACGCTGCAG TCCGGATCCT TCAGCAGCTT CTGCCACAGT 
AAAATCCAGA GCGCTCCGAC CGACGCCGAG CAGGACATCT GGAGGTTCCT CATGGTGAAT 
TTTGAGGATG AAGCGCGTGT GAAGTTTTTG AGGCTTCTGG GATTCAGTAA AGACGAGCTG 
CAGAGAAAGA TCTCCGCTTG TTTGGGAAGG AAACTTCAAC CCAACGGTTT AGATGTGAAT 
GACCTTGCAG AGAAGATGCA GACACTTTCA GATCAGAGAG CAGATGAGTG CGCCGGAGCT 
GCTGACGGTT TGCCCGGTCG TGAATCTCCT GCTGATTTCT TCAGTCAGCT GCCGAAGAAA 
GAGAAAGACA AACAGCCCTT CCAGATCCCC GTATCCTCAG ATACAGACGG TTTGATCAGT 
CAGGCGCTGT TAGTGGGAAA CTTCGAGGGC GCTGTGGATC TGTGTCTGAA TGACGGTCGG 
TTTGCGGAGG CCATCCTTCT GTCCATCAGC GGAGGAGAAG AACTGCTGAA GAAAACCCAG 
CAGAGATACC TGCAGCGACA GACCAACAGC ATCTCCATGC TGATCTCGTC AGTAGTGACA 
CAGAACTGGC GTGATATCGT TCAGAGCTGT GAGCTTGATA ACTGGAAGGA GGCGTTAGCA 
GCGTTGCTAA CCTACGCTAA CCCCGAGGAG TTTGCTAGTC TGTGCGATAC TCTGGGCTCT 
CGTCTGGAGG CTGGCGGCTC AGAGAAGCTC TGTCTGCAGG CCTGTCTGTG CTTCATCTGC 
TCGGGGAACA TCGAGCGTCT GGTGGACTGC TGGACACTGC AGAGAGACTG CTCATCACCA 
CTCGCTCTCG AGGATCTGGT GGAGAAGGTG ATGATCCTGA GGAAGTCTAT TGAGCGTCTG 
CGTAACATTG AGGTGTGTGT GCGCAGCTCA GCGCTGACCC AGAAGCTCAT CCAGTACTCC 
AGTATTCTGG CATCTCAGGG CAGCCTGAGC GCAGCACTGA ACTACCTGCC CAGTGACTCC 
GACCAGGCTG CAGTGCGGAT GTTGAGGGAC AGACTGTCAC ACGCCAAAGG AGAAATACAG 
CAGAAACCCT CAGCTCCAGC AGGAGGCGCT AAACCTGCAC CAAACACACA CACTCCTGCA 
GCACACACAA CGGCGCCATA CCCGTCCTCG GGGGCCCCTC AGATGCCACG TCTCTTCAGT 
CCTCAGACCG GCCCCGAACC TCCTGCTGGA GCAACAACAC ACACACCCAG ACCAGCATAC 
ACACAACACC CTGCTACAGC CCCAGGTTTT CATCCGTTTC CTCCGGCCGC TCCTCAGGGT 
GCTCCTGGTG GTTTCTTCAC TCCTCAGATG CCCTCCTCCA GCCTGGCGGG TCCCTCAATG 
CCGCCCTCGT CTCCTCCGGG CCCCATGATG CCCGCTGGTG TTCCCATGAT GATGCCCACT 
CCTCCAGCTC CAGGCTTCCT CTCGCCCACG TCTCTCCCCG GCGGGCCGAG GTTTCCAGGA 
TCTGCGCACA CACAGGGCCC CGCTCCTCCT CCCACCGGGG CGTATCCGTC TCTGGGGGCC 
GCCGGGTATC CACAGGGTGG CCCCGGAGCT CCTGCAGCCA AGAGCTTCAG CGGGGCCCCC 
GTCCCTCCGC CACCCTCAGG GGCTCAGGAT GGATGGAATG ACCCTCCGGC TGTGCGTGCA 
GGACAGCGCA AGAAAAAGCT GCCAGAGAAC TACACTCCTC CAGCACCGAT TACAGCTCCA 
GTGATGGGTT TCCCAGCGGA GGCTCCAGTG CTTCAGGATC GTGTGCAGGT GCCACCCGGA 
GCCCCTCAGG AGCCCAGCAT ACAGTCCATA CAGCAGCTTC CTGCCGAGCG TGTGGAGCAG 
AAAGAAATCC CAGCCGAACA CATGATCCTG AAGACCACCT TCAGCAGTCT GGTGCAGCGC 
TGTCAGCTGG CCGCCGCAGA CCCGCAAACG AAACGCAAGC TTGATGACGC CTCCAAACGT 
CTCGAGAATC TGTATGACAA ACTGCGAGAT CAATCGCTTT CTCCCAGCAT CCTCGGCGGT 
CTCCACGAGA TTAGCCGGTG TGTTAGCGCA CGCAGCTATG TGCAAGGTCT GGAGGTGCAC 
ACGCAGGTGG TGAGCAGCAG CAACTTCAGC GAGATCTCGG CCTTCATGCC CGTCCTCAAA 
GTGCTCATGA CCATCGCCAA CAAGCTGGCC GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001921581.3
CDS	131..3646
Translation	MRVKEILRTATQAWSPARQHPAYLALGTAAQQLDASFNTSSALEIFDMDFSDTSMDMKLRGTLATSNRFHSLIWVAFGMDVQPSSGRLIGGSENGSVTVFNPDVILTAGEDAVIGQSNKHTGPVRALDFNRFQSNLIASGANDSEIYIWDLNNFSNPMTPGTKSQPAEDVSVVAWNSQVQHILASANPSGKAVVWDLRKNEPIIKISDHSNRMHCSGMLWHPDVATQLVLASEDDRLPVIQMWDLRFATSPLKVLENHTRGILSISWSQADPELLLSSGKDNRILCWNPNTGEVIYELPTANQWCFDVQWCPRNPALLSAASFDGRISVYSVMGGRLQQQQHSTVDQISASFDTNDPFGMGQTLPPLQVPQAAVETTIIPPLKKPPKWVRRPVGASFAFGGKLVTFENPNVAPPQQQQPANPLPRQVHVSQVTTETEFLQRSTELQMTLQSGSFSSFCHSKIQSAPTDAEQDIWRFLMVNFEDEARVKFLRLLGFSKDELQRKISACLGRKLQPNGLDVNDLAEKMQTLSDQRADECAGAADGLPGRESPADFFSQLPKKEKDKQPFQIPVSSDTDGLISQALLVGNFEGAVDLCLNDGRFAEAILLSISGGEELLKKTQQRYLQRQTNSISMLISSVVTQNWRDIVQSCELDNWKEALAALLTYANPEEFASLCDTLGSRLEAGGSEKLCLQACLCFICSGNIERLVDCWTLQRDCSSPLALEDLVEKVMILRKSIERLRNIEVCVRSSALTQKLIQYSSILASQGSLSAALNYLPSDSDQAAVRMLRDRLSHAKGEIQQKPSAPAGGAKPAPNTHTPAAHTTAPYPSSGAPQMPRLFSPQTGPEPPAGATTHTPRPAYTQHPATAPGFHPFPPAAPQGAPGGFFTPQMPSSSLAGPSMPPSSPPGPMMPAGVPMMMPTPPAPGFLSPTSLPGGPRFPGSAHTQGPAPPPTGAYPSLGAAGYPQGGPGAPAAKSFSGAPVPPPPSGAQDGWNDPPAVRAGQRKKKLPENYTPPAPITAPVMGFPAEAPVLQDRVQVPPGAPQEPSIQSIQQLPAERVEQKEIPAEHMILKTTFSSLVQRCQLAAADPQTKRKLDDASKRLENLYDKLRDQSLSPSILGGLHEISRCVSARSYVQGLEVHTQVVSSSNFSEISAFMPVLKVLMTIANKLAV

Target ORF information:

RefSeq Version	XM_001921546.7
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SEC31 homolog B, COPII coat complex component (sec31b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001921546.7

ORF Insert Sequence:

ATGAGGGTGA AGGAGATCCT CAGGACGGCC ACACAGGCGT GGAGTCCTGC CAGACAACAC 
CCGGCCTACC TGGCCTTAGG CACCGCAGCG CAGCAGTTAG ACGCCTCCTT CAACACCAGC 
TCTGCCCTGG AGATCTTCGA TATGGATTTT TCAGATACGT CAATGGACAT GAAGCTCAGA 
GGAACTCTGG CCACCTCCAA CAGGTTTCAC AGTCTGATCT GGGTGGCGTT TGGTATGGAT 
GTCCAGCCGT CGTCGGGTCG TCTCATCGGA GGCAGTGAGA ATGGTAGCGT GACCGTCTTC 
AATCCCGACG TGATTCTGAC CGCAGGAGAG GATGCTGTGA TTGGCCAGTC TAATAAACAC 
ACGGGACCTG TTCGAGCCCT CGACTTCAAC CGCTTTCAGA GTAATCTGAT CGCATCAGGA 
GCCAATGACT CAGAGATCTA CATCTGGGAC CTGAACAACT TCAGCAATCC AATGACTCCC 
GGCACAAAGT CACAGCCGGC GGAGGACGTG AGCGTGGTGG CCTGGAACAG TCAAGTGCAG 
CACATTCTGG CCTCCGCAAA CCCCAGCGGG AAAGCAGTGG TGTGGGACCT GAGGAAGAAC 
GAGCCCATCA TCAAAATCAG CGACCACAGC AACAGGATGC ACTGCTCAGG GATGCTGTGG 
CATCCAGATG TGGCCACTCA GCTGGTTCTG GCCTCAGAGG ACGACAGGCT GCCAGTCATC 
CAGATGTGGG ATCTTCGGTT CGCAACATCA CCTCTAAAAG TGCTGGAGAA CCACACCAGA 
GGGATTTTGT CGATCTCATG GAGTCAAGCT GATCCTGAGC TCCTGCTGAG TAGCGGGAAA 
GACAACCGCA TCCTGTGCTG GAACCCCAAC ACTGGAGAGG TGATTTATGA GCTGCCGACA 
GCCAATCAGT GGTGTTTCGA TGTGCAGTGG TGTCCTCGAA ACCCCGCCCT CCTGTCAGCC 
GCATCGTTTG ACGGACGCAT CAGTGTTTAC TCTGTTATGG GTGGACGACT GCAGCAGCAG 
CAGCACAGCA CAGTCGACCA GATCTCCGCT TCGTTTGATA CTAATGACCC ATTTGGGATG 
GGTCAAACTC TGCCGCCTCT TCAGGTTCCT CAAGCCGCTG TGGAGACCAC CATTATTCCT 
CCGTTAAAAA AGCCTCCAAA GTGGGTGCGG AGGCCTGTAG GAGCATCTTT CGCTTTCGGT 
GGGAAACTGG TGACCTTTGA GAACCCTAAC GTGGCTCCGC CCCAGCAACA ACAACCAGCC 
AATCCACTCC CTCGACAGGT GCATGTGAGT CAGGTTACCA CAGAGACAGA GTTCCTCCAG 
CGCTCCACTG AGCTGCAGAT GACGCTGCAG TCCGGATCCT TCAGCAGCTT CTGCCACAGT 
AAAATCCAGA GCGCTCCGAC CGACGCCGAG CAGGACATCT GGAGGTTCCT CATGGTGAAT 
TTTGAGGATG AAGCGCGTGT GAAGTTTTTG AGGCTTCTGG GATTCAGTAA AGACGAGCTG 
CAGAGAAAGA TCTCCGCTTG TTTGGGAAGG AAACTTCAAC CCAACGGTTT AGATGTGAAT 
GACCTTGCAG AGAAGATGCA GACACTTTCA GATCAGAGAG CAGATGAGTG CGCCGGAGCT 
GCTGACGGTT TGCCCGGTCG TGAATCTCCT GCTGATTTCT TCAGTCAGCT GCCGAAGAAA 
GAGAAAGACA AACAGCCCTT CCAGATCCCC GTATCCTCAG ATACAGACGG TTTGATCAGT 
CAGGCGCTGT TAGTGGGAAA CTTCGAGGGC GCTGTGGATC TGTGTCTGAA TGACGGTCGG 
TTTGCGGAGG CCATCCTTCT GTCCATCAGC GGAGGAGAAG AACTGCTGAA GAAAACCCAG 
CAGAGATACC TGCAGCGACA GACCAACAGC ATCTCCATGC TGATCTCGTC AGTAGTGACA 
CAGAACTGGC GTGATATCGT TCAGAGCTGT GAGCTTGATA ACTGGAAGGA GGCGTTAGCA 
GCGTTGCTAA CCTACGCTAA CCCCGAGGAG TTTGCTAGTC TGTGCGATAC TCTGGGCTCT 
CGTCTGGAGG CTGGCGGCTC AGAGAAGCTC TGTCTGCAGG CCTGTCTGTG CTTCATCTGC 
TCGGGGAACA TCGAGCGTCT GGTGGACTGC TGGACACTGC AGAGAGACTG CTCATCACCA 
CTCGCTCTCG AGGATCTGGT GGAGAAGGTG ATGATCCTGA GGAAGTCTAT TGAGCGTCTG 
CGTAACATTG AGGTGTGTGT GCGCAGCTCA GCGCTGACCC AGAAGCTCAT CCAGTACTCC 
AGTATTCTGG CATCTCAGGG CAGCCTGAGC GCAGCACTGA ACTACCTGCC CAGTGACTCC 
GACCAGGCTG CAGTGCGGAT GTTGAGGGAC AGACTGTCAC ACGCCAAAGG AGAAATACAG 
CAGAAACCCT CAGCTCCAGC AGGAGGCGCT AAACCTGCAC CAAACACACA CACTCCTGCA 
GCACACACAA CGGCGCCATA CCCGTCCTCG GGGGCCCCTC AGATGCCACG TCTCTTCAGT 
CCTCAGACCG GCCCCGAACC TCCTGCTGGA GCAACAACAC ACACACCCAG ACCAGCATAC 
ACACAACACC CTGCTACAGC CCCAGGTTTT CATCCGTTTC CTCCGGCCGC TCCTCAGGGT 
GCTCCTGGTG GTTTCTTCAC TCCTCAGATG CCCTCCTCCA GCCTGGCGGG TCCCTCAATG 
CCGCCCTCGT CTCCTCCGGG CCCCATGATG CCCGCTGGTG TTCCCATGAT GATGCCCACT 
CCTCCAGCTC CAGGCTTCCT CTCGCCCACG TCTCTCCCCG GCGGGCCGAG GTTTCCAGGA 
TCTGCGCACA CACAGGGCCC CGCTCCTCCT CCCACCGGGG CGTATCCGTC TCTGGGGGCC 
GCCGGGTATC CACAGGGTGG CCCCGGAGCT CCTGCAGCCA AGAGCTTCAG CGGGGCCCCC 
GTCCCTCCGC CACCCTCAGG GGCTCAGGAT GGATGGAATG ACCCTCCGGC TGTGCGTGCA 
GGACAGCGCA AGAAAAAGCT GCCAGAGAAC TACACTCCTC CAGCACCGAT TACAGCTCCA 
GTGATGGGTT TCCCAGCGGA GGCTCCAGTG CTTCAGGATC GTGTGCAGGT GCCACCCGGA 
GCCCCTCAGG AGCCCAGCAT ACAGTCCATA CAGCAGCTTC CTGCCGAGCG TGTGGAGCAG 
AAAGAAATCC CAGCCGAACA CATGATCCTG AAGACCACCT TCAGCAGTCT GGTGCAGCGC 
TGTCAGCTGG CCGCCGCAGA CCCGCAAACG AAACGCAAGC TTGATGACGC CTCCAAACGT 
CTCGAGAATC TGTATGACAA ACTGCGAGAT CAATCGCTTT CTCCCAGCAT CCTCGGCGGT 
CTCCACGAGA TTAGCCGGTG TGTTAGCGCA CGCAGCTATG TGCAAGGTCT GGAGGTGCAC 
ACGCAGGTGG TGAGCAGCAG CAACTTCAGC GAGATCTCGG CCTTCATGCC CGTCCTCAAA 
GTGCTCATGA CCATCGCCAA CAAGCTGGCC GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sec31b ( XM_001921546.6 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001921581.3 Danio rerio SEC31 homolog B, COPII coat complex component (sec31b), transcript variant X3, mRNA.

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CloneID	ODa18302
Clone ID Related Accession (Same CDS sequence)	XM_001921546.7 , XM_001921546.6
Accession Version	XM_001921546.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein transport protein Sec31A isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001921546.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGTGA AGGAGATCCT CAGGACGGCC ACACAGGCGT GGAGTCCTGC CAGACAACAC 
CCGGCCTACC TGGCCTTAGG CACCGCAGCG CAGCAGTTAG ACGCCTCCTT CAACACCAGC 
TCTGCCCTGG AGATCTTCGA TATGGATTTT TCAGATACGT CAATGGACAT GAAGCTCAGA 
GGAACTCTGG CCACCTCCAA CAGGTTTCAC AGTCTGATCT GGGTGGCGTT TGGTATGGAT 
GTCCAGCCGT CGTCGGGTCG TCTCATCGGA GGCAGTGAGA ATGGTAGCGT GACCGTCTTC 
AATCCCGACG TGATTCTGAC CGCAGGAGAG GATGCTGTGA TTGGCCAGTC TAATAAACAC 
ACGGGACCTG TTCGAGCCCT CGACTTCAAC CGCTTTCAGA GTAATCTGAT CGCATCAGGA 
GCCAATGACT CAGAGATCTA CATCTGGGAC CTGAACAACT TCAGCAATCC AATGACTCCC 
GGCACAAAGT CACAGCCGGC GGAGGACGTG AGCGTGGTGG CCTGGAACAG TCAAGTGCAG 
CACATTCTGG CCTCCGCAAA CCCCAGCGGG AAAGCAGTGG TGTGGGACCT GAGGAAGAAC 
GAGCCCATCA TCAAAATCAG CGACCACAGC AACAGGATGC ACTGCTCAGG GATGCTGTGG 
CATCCAGATG TGGCCACTCA GCTGGTTCTG GCCTCAGAGG ACGACAGGCT GCCAGTCATC 
CAGATGTGGG ATCTTCGGTT CGCAACATCA CCTCTAAAAG TGCTGGAGAA CCACACCAGA 
GGGATTTTGT CGATCTCATG GAGTCAAGCT GATCCTGAGC TCCTGCTGAG TAGCGGGAAA 
GACAACCGCA TCCTGTGCTG GAACCCCAAC ACTGGAGAGG TGATTTATGA GCTGCCGACA 
GCCAATCAGT GGTGTTTCGA TGTGCAGTGG TGTCCTCGAA ACCCCGCCCT CCTGTCAGCC 
GCATCGTTTG ACGGACGCAT CAGTGTTTAC TCTGTTATGG GTGGACGACT GCAGCAGCAG 
CAGCACAGCA CAGTCGACCA GATCTCCGCT TCGTTTGATA CTAATGACCC ATTTGGGATG 
GGTCAAACTC TGCCGCCTCT TCAGGTTCCT CAAGCCGCTG TGGAGACCAC CATTATTCCT 
CCGTTAAAAA AGCCTCCAAA GTGGGTGCGG AGGCCTGTAG GAGCATCTTT CGCTTTCGGT 
GGGAAACTGG TGACCTTTGA GAACCCTAAC GTGGCTCCGC CCCAGCAACA ACAACCAGCC 
AATCCACTCC CTCGACAGGT GCATGTGAGT CAGGTTACCA CAGAGACAGA GTTCCTCCAG 
CGCTCCACTG AGCTGCAGAT GACGCTGCAG TCCGGATCCT TCAGCAGCTT CTGCCACAGT 
AAAATCCAGA GCGCTCCGAC CGACGCCGAG CAGGACATCT GGAGGTTCCT CATGGTGAAT 
TTTGAGGATG AAGCGCGTGT GAAGTTTTTG AGGCTTCTGG GATTCAGTAA AGACGAGCTG 
CAGAGAAAGA TCTCCGCTTG TTTGGGAAGG AAACTTCAAC CCAACGGTTT AGATGTGAAT 
GACCTTGCAG AGAAGATGCA GACACTTTCA GATCAGAGAG CAGATGAGTG CGCCGGAGCT 
GCTGACGGTT TGCCCGGTCG TGAATCTCCT GCTGATTTCT TCAGTCAGCT GCCGAAGAAA 
GAGAAAGACA AACAGCCCTT CCAGATCCCC GTATCCTCAG ATACAGACGG TTTGATCAGT 
CAGGCGCTGT TAGTGGGAAA CTTCGAGGGC GCTGTGGATC TGTGTCTGAA TGACGGTCGG 
TTTGCGGAGG CCATCCTTCT GTCCATCAGC GGAGGAGAAG AACTGCTGAA GAAAACCCAG 
CAGAGATACC TGCAGCGACA GACCAACAGC ATCTCCATGC TGATCTCGTC AGTAGTGACA 
CAGAACTGGC GTGATATCGT TCAGAGCTGT GAGCTTGATA ACTGGAAGGA GGCGTTAGCA 
GCGTTGCTAA CCTACGCTAA CCCCGAGGAG TTTGCTAGTC TGTGCGATAC TCTGGGCTCT 
CGTCTGGAGG CTGGCGGCTC AGAGAAGCTC TGTCTGCAGG CCTGTCTGTG CTTCATCTGC 
TCGGGGAACA TCGAGCGTCT GGTGGACTGC TGGACACTGC AGAGAGACTG CTCATCACCA 
CTCGCTCTCG AGGATCTGGT GGAGAAGGTG ATGATCCTGA GGAAGTCTAT TGAGCGTCTG 
CGTAACATTG AGGTGTGTGT GCGCAGCTCA GCGCTGACCC AGAAGCTCAT CCAGTACTCC 
AGTATTCTGG CATCTCAGGG CAGCCTGAGC GCAGCACTGA ACTACCTGCC CAGTGACTCC 
GACCAGGCTG CAGTGCGGAT GTTGAGGGAC AGACTGTCAC ACGCCAAAGG AGAAATACAG 
CAGAAACCCT CAGCTCCAGC AGGAGGCGCT AAACCTGCAC CAAACACACA CACTCCTGCA 
GCACACACAA CGGCGCCATA CCCGTCCTCG GGGGCCCCTC AGATGCCACG TCTCTTCAGT 
CCTCAGACCG GCCCCGAACC TCCTGCTGGA GCAACAACAC ACACACCCAG ACCAGCATAC 
ACACAACACC CTGCTACAGC CCCAGGTTTT CATCCGTTTC CTCCGGCCGC TCCTCAGGGT 
GCTCCTGGTG GTTTCTTCAC TCCTCAGATG CCCTCCTCCA GCCTGGCGGG TCCCTCAATG 
CCGCCCTCGT CTCCTCCGGG CCCCATGATG CCCGCTGGTG TTCCCATGAT GATGCCCACT 
CCTCCAGCTC CAGGCTTCCT CTCGCCCACG TCTCTCCCCG GCGGGCCGAG GTTTCCAGGA 
TCTGCGCACA CACAGGGCCC CGCTCCTCCT CCCACCGGGG CGTATCCGTC TCTGGGGGCC 
GCCGGGTATC CACAGGGTGG CCCCGGAGCT CCTGCAGCCA AGAGCTTCAG CGGGGCCCCC 
GTCCCTCCGC CACCCTCAGG GGCTCAGGAT GGATGGAATG ACCCTCCGGC TGTGCGTGCA 
GGACAGCGCA AGAAAAAGCT GCCAGAGAAC TACACTCCTC CAGCACCGAT TACAGCTCCA 
GTGATGGGTT TCCCAGCGGA GGCTCCAGTG CTTCAGGATC GTGTGCAGGT GCCACCCGGA 
GCCCCTCAGG AGCCCAGCAT ACAGTCCATA CAGCAGCTTC CTGCCGAGCG TGTGGAGCAG 
AAAGAAATCC CAGCCGAACA CATGATCCTG AAGACCACCT TCAGCAGTCT GGTGCAGCGC 
TGTCAGCTGG CCGCCGCAGA CCCGCAAACG AAACGCAAGC TTGATGACGC CTCCAAACGT 
CTCGAGAATC TGTATGACAA ACTGCGAGAT CAATCGCTTT CTCCCAGCAT CCTCGGCGGT 
CTCCACGAGA TTAGCCGGTG TGTTAGCGCA CGCAGCTATG TGCAAGGTCT GGAGGTGCAC 
ACGCAGGTGG TGAGCAGCAG CAACTTCAGC GAGATCTCGG CCTTCATGCC CGTCCTCAAA 
GTGCTCATGA CCATCGCCAA CAAGCTGGCC GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001921581.3
CDS	90..3605
Translation	MRVKEILRTATQAWSPARQHPAYLALGTAAQQLDASFNTSSALEIFDMDFSDTSMDMKLRGTLATSNRFHSLIWVAFGMDVQPSSGRLIGGSENGSVTVFNPDVILTAGEDAVIGQSNKHTGPVRALDFNRFQSNLIASGANDSEIYIWDLNNFSNPMTPGTKSQPAEDVSVVAWNSQVQHILASANPSGKAVVWDLRKNEPIIKISDHSNRMHCSGMLWHPDVATQLVLASEDDRLPVIQMWDLRFATSPLKVLENHTRGILSISWSQADPELLLSSGKDNRILCWNPNTGEVIYELPTANQWCFDVQWCPRNPALLSAASFDGRISVYSVMGGRLQQQQHSTVDQISASFDTNDPFGMGQTLPPLQVPQAAVETTIIPPLKKPPKWVRRPVGASFAFGGKLVTFENPNVAPPQQQQPANPLPRQVHVSQVTTETEFLQRSTELQMTLQSGSFSSFCHSKIQSAPTDAEQDIWRFLMVNFEDEARVKFLRLLGFSKDELQRKISACLGRKLQPNGLDVNDLAEKMQTLSDQRADECAGAADGLPGRESPADFFSQLPKKEKDKQPFQIPVSSDTDGLISQALLVGNFEGAVDLCLNDGRFAEAILLSISGGEELLKKTQQRYLQRQTNSISMLISSVVTQNWRDIVQSCELDNWKEALAALLTYANPEEFASLCDTLGSRLEAGGSEKLCLQACLCFICSGNIERLVDCWTLQRDCSSPLALEDLVEKVMILRKSIERLRNIEVCVRSSALTQKLIQYSSILASQGSLSAALNYLPSDSDQAAVRMLRDRLSHAKGEIQQKPSAPAGGAKPAPNTHTPAAHTTAPYPSSGAPQMPRLFSPQTGPEPPAGATTHTPRPAYTQHPATAPGFHPFPPAAPQGAPGGFFTPQMPSSSLAGPSMPPSSPPGPMMPAGVPMMMPTPPAPGFLSPTSLPGGPRFPGSAHTQGPAPPPTGAYPSLGAAGYPQGGPGAPAAKSFSGAPVPPPPSGAQDGWNDPPAVRAGQRKKKLPENYTPPAPITAPVMGFPAEAPVLQDRVQVPPGAPQEPSIQSIQQLPAERVEQKEIPAEHMILKTTFSSLVQRCQLAAADPQTKRKLDDASKRLENLYDKLRDQSLSPSILGGLHEISRCVSARSYVQGLEVHTQVVSSSNFSEISAFMPVLKVLMTIANKLAV

Target ORF information:

RefSeq Version	XM_001921546.6
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio SEC31 homolog B, COPII coat complex component (sec31b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001921546.6

ORF Insert Sequence:

ATGAGGGTGA AGGAGATCCT CAGGACGGCC ACACAGGCGT GGAGTCCTGC CAGACAACAC 
CCGGCCTACC TGGCCTTAGG CACCGCAGCG CAGCAGTTAG ACGCCTCCTT CAACACCAGC 
TCTGCCCTGG AGATCTTCGA TATGGATTTT TCAGATACGT CAATGGACAT GAAGCTCAGA 
GGAACTCTGG CCACCTCCAA CAGGTTTCAC AGTCTGATCT GGGTGGCGTT TGGTATGGAT 
GTCCAGCCGT CGTCGGGTCG TCTCATCGGA GGCAGTGAGA ATGGTAGCGT GACCGTCTTC 
AATCCCGACG TGATTCTGAC CGCAGGAGAG GATGCTGTGA TTGGCCAGTC TAATAAACAC 
ACGGGACCTG TTCGAGCCCT CGACTTCAAC CGCTTTCAGA GTAATCTGAT CGCATCAGGA 
GCCAATGACT CAGAGATCTA CATCTGGGAC CTGAACAACT TCAGCAATCC AATGACTCCC 
GGCACAAAGT CACAGCCGGC GGAGGACGTG AGCGTGGTGG CCTGGAACAG TCAAGTGCAG 
CACATTCTGG CCTCCGCAAA CCCCAGCGGG AAAGCAGTGG TGTGGGACCT GAGGAAGAAC 
GAGCCCATCA TCAAAATCAG CGACCACAGC AACAGGATGC ACTGCTCAGG GATGCTGTGG 
CATCCAGATG TGGCCACTCA GCTGGTTCTG GCCTCAGAGG ACGACAGGCT GCCAGTCATC 
CAGATGTGGG ATCTTCGGTT CGCAACATCA CCTCTAAAAG TGCTGGAGAA CCACACCAGA 
GGGATTTTGT CGATCTCATG GAGTCAAGCT GATCCTGAGC TCCTGCTGAG TAGCGGGAAA 
GACAACCGCA TCCTGTGCTG GAACCCCAAC ACTGGAGAGG TGATTTATGA GCTGCCGACA 
GCCAATCAGT GGTGTTTCGA TGTGCAGTGG TGTCCTCGAA ACCCCGCCCT CCTGTCAGCC 
GCATCGTTTG ACGGACGCAT CAGTGTTTAC TCTGTTATGG GTGGACGACT GCAGCAGCAG 
CAGCACAGCA CAGTCGACCA GATCTCCGCT TCGTTTGATA CTAATGACCC ATTTGGGATG 
GGTCAAACTC TGCCGCCTCT TCAGGTTCCT CAAGCCGCTG TGGAGACCAC CATTATTCCT 
CCGTTAAAAA AGCCTCCAAA GTGGGTGCGG AGGCCTGTAG GAGCATCTTT CGCTTTCGGT 
GGGAAACTGG TGACCTTTGA GAACCCTAAC GTGGCTCCGC CCCAGCAACA ACAACCAGCC 
AATCCACTCC CTCGACAGGT GCATGTGAGT CAGGTTACCA CAGAGACAGA GTTCCTCCAG 
CGCTCCACTG AGCTGCAGAT GACGCTGCAG TCCGGATCCT TCAGCAGCTT CTGCCACAGT 
AAAATCCAGA GCGCTCCGAC CGACGCCGAG CAGGACATCT GGAGGTTCCT CATGGTGAAT 
TTTGAGGATG AAGCGCGTGT GAAGTTTTTG AGGCTTCTGG GATTCAGTAA AGACGAGCTG 
CAGAGAAAGA TCTCCGCTTG TTTGGGAAGG AAACTTCAAC CCAACGGTTT AGATGTGAAT 
GACCTTGCAG AGAAGATGCA GACACTTTCA GATCAGAGAG CAGATGAGTG CGCCGGAGCT 
GCTGACGGTT TGCCCGGTCG TGAATCTCCT GCTGATTTCT TCAGTCAGCT GCCGAAGAAA 
GAGAAAGACA AACAGCCCTT CCAGATCCCC GTATCCTCAG ATACAGACGG TTTGATCAGT 
CAGGCGCTGT TAGTGGGAAA CTTCGAGGGC GCTGTGGATC TGTGTCTGAA TGACGGTCGG 
TTTGCGGAGG CCATCCTTCT GTCCATCAGC GGAGGAGAAG AACTGCTGAA GAAAACCCAG 
CAGAGATACC TGCAGCGACA GACCAACAGC ATCTCCATGC TGATCTCGTC AGTAGTGACA 
CAGAACTGGC GTGATATCGT TCAGAGCTGT GAGCTTGATA ACTGGAAGGA GGCGTTAGCA 
GCGTTGCTAA CCTACGCTAA CCCCGAGGAG TTTGCTAGTC TGTGCGATAC TCTGGGCTCT 
CGTCTGGAGG CTGGCGGCTC AGAGAAGCTC TGTCTGCAGG CCTGTCTGTG CTTCATCTGC 
TCGGGGAACA TCGAGCGTCT GGTGGACTGC TGGACACTGC AGAGAGACTG CTCATCACCA 
CTCGCTCTCG AGGATCTGGT GGAGAAGGTG ATGATCCTGA GGAAGTCTAT TGAGCGTCTG 
CGTAACATTG AGGTGTGTGT GCGCAGCTCA GCGCTGACCC AGAAGCTCAT CCAGTACTCC 
AGTATTCTGG CATCTCAGGG CAGCCTGAGC GCAGCACTGA ACTACCTGCC CAGTGACTCC 
GACCAGGCTG CAGTGCGGAT GTTGAGGGAC AGACTGTCAC ACGCCAAAGG AGAAATACAG 
CAGAAACCCT CAGCTCCAGC AGGAGGCGCT AAACCTGCAC CAAACACACA CACTCCTGCA 
GCACACACAA CGGCGCCATA CCCGTCCTCG GGGGCCCCTC AGATGCCACG TCTCTTCAGT 
CCTCAGACCG GCCCCGAACC TCCTGCTGGA GCAACAACAC ACACACCCAG ACCAGCATAC 
ACACAACACC CTGCTACAGC CCCAGGTTTT CATCCGTTTC CTCCGGCCGC TCCTCAGGGT 
GCTCCTGGTG GTTTCTTCAC TCCTCAGATG CCCTCCTCCA GCCTGGCGGG TCCCTCAATG 
CCGCCCTCGT CTCCTCCGGG CCCCATGATG CCCGCTGGTG TTCCCATGAT GATGCCCACT 
CCTCCAGCTC CAGGCTTCCT CTCGCCCACG TCTCTCCCCG GCGGGCCGAG GTTTCCAGGA 
TCTGCGCACA CACAGGGCCC CGCTCCTCCT CCCACCGGGG CGTATCCGTC TCTGGGGGCC 
GCCGGGTATC CACAGGGTGG CCCCGGAGCT CCTGCAGCCA AGAGCTTCAG CGGGGCCCCC 
GTCCCTCCGC CACCCTCAGG GGCTCAGGAT GGATGGAATG ACCCTCCGGC TGTGCGTGCA 
GGACAGCGCA AGAAAAAGCT GCCAGAGAAC TACACTCCTC CAGCACCGAT TACAGCTCCA 
GTGATGGGTT TCCCAGCGGA GGCTCCAGTG CTTCAGGATC GTGTGCAGGT GCCACCCGGA 
GCCCCTCAGG AGCCCAGCAT ACAGTCCATA CAGCAGCTTC CTGCCGAGCG TGTGGAGCAG 
AAAGAAATCC CAGCCGAACA CATGATCCTG AAGACCACCT TCAGCAGTCT GGTGCAGCGC 
TGTCAGCTGG CCGCCGCAGA CCCGCAAACG AAACGCAAGC TTGATGACGC CTCCAAACGT 
CTCGAGAATC TGTATGACAA ACTGCGAGAT CAATCGCTTT CTCCCAGCAT CCTCGGCGGT 
CTCCACGAGA TTAGCCGGTG TGTTAGCGCA CGCAGCTATG TGCAAGGTCT GGAGGTGCAC 
ACGCAGGTGG TGAGCAGCAG CAACTTCAGC GAGATCTCGG CCTTCATGCC CGTCCTCAAA 
GTGCTCATGA CCATCGCCAA CAAGCTGGCC GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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sec31b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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