dgkh cDNA ORF clone, Danio rerio(zebrafish)

The following dgkh gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dgkh cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa145029 XM_021474623.1
Latest version!
Danio rerio diacylglycerol kinase, eta (dgkh), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00
ODa145029 XM_021472147.1
Latest version!
Danio rerio diacylglycerol kinase, eta (dgkh), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00
ODa145030 XM_021474624.1
Latest version!
Danio rerio diacylglycerol kinase, eta (dgkh), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00
ODa145030 XM_021472148.1
Latest version!
Danio rerio diacylglycerol kinase, eta (dgkh), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00
ODa145031 XM_021474625.1
Latest version!
Danio rerio diacylglycerol kinase, eta (dgkh), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $104.30
$149.00
ODa145031 XM_021472149.1
Latest version!
Danio rerio diacylglycerol kinase, eta (dgkh), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $104.30
$149.00
ODa15020 XM_009304586.2
Latest version!
Danio rerio diacylglycerol kinase, eta (dgkh), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $629.30
$899.00
ODa48522 XM_009304583.3
Latest version!
Danio rerio diacylglycerol kinase, eta (dgkh), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $713.30
$1019.00
ODa48523 XM_009304584.2
Latest version!
Danio rerio diacylglycerol kinase, eta (dgkh), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $713.30
$1019.00
ODa48524 XM_009304585.3
Latest version!
Danio rerio diacylglycerol kinase, eta (dgkh), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $713.30
$1019.00
ODa144060 XM_021478876.1
Latest version!
Danio rerio diacylglycerol kinase, eta (dgkh), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa144061 XM_021478877.1
Latest version!
Danio rerio diacylglycerol kinase, eta (dgkh), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa15017 XM_005167979.4
Latest version!
Danio rerio diacylglycerol kinase, eta (dgkh), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
ODa15017 XM_005167979.3 Danio rerio diacylglycerol kinase, eta (dgkh), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa48522 XM_009304583.2 Danio rerio diacylglycerol kinase, eta (dgkh), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $713.30
$1019.00
ODa48524 XM_009304585.2 Danio rerio diacylglycerol kinase, eta (dgkh), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa145029
    Clone ID Related Accession (Same CDS sequence) XM_021472147.1 , XM_021474623.1
    Accession Version XM_021472147.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 741bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product diacylglycerol kinase eta isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394746.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGACGGCCA AGAGAACCGC TTTCCAGCCG TTGTCCAGCT CCCGCATCCC AGCGGAGAGG 
    AAGGGAGCGG CTGAGCGCGT TCCTAACCCG GAGTGCTGTG ACTTTCCCCC GCAGGAGCCG
    GAGAAAGCAG CTGCGTCCGC CTCGGGGGTT CCCGCCGTGG GGGCTGTCGG GGGCTCGGGG
    CCGGTGGTGG ACGAGTCATC CGACAGCGAA GGAGAGCAGG AAGGGCCGCA GAAACTCATC
    AGGAAAGTGT CAACCTCCGG GCAAATACGA AGCAAGACAA GCGTGAAGGA AGGGCTTCTT
    CTGAAACAGA CAAGCTCGTT CCAGCGTTGG AAAAAACGTT ATTTCAAGCT ACGAGGACGG
    ACGCTCTACT ATGCTAAGGA TGCCAAGGCG GGTGGCGTTT TGGCCCGCTG GCAGTATCGG
    GTCTCTCGTA GCCGCGTGTG CTGGAGGGTG CTTTCCGTGT GCTGGACCGG TGCCCGCCGT
    CCGCCGCCCG CTCTTGGCGC CAGTGGATCG GAGGAAGGCT TGGTGGCGAT TCGGTCTCTT
    ATTTTCGATG AGGTGGACCT TTCAGATGCC AGTGTTGCCG AATCAAGTAC AAAGAATATC
    AACAACAGTT TCACGGTAAG AGGCCATTTC CAGGAATGCT GCAAATATCC ACAATATACA
    GCAAATATTA AGAGAGTACA TTATAAGTGT AACATTAACT ACAAGACCAC CAGATTCTAC
    AAGATCACGC TGTTCTCATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327822.1
    CDS803..1543
    Translation

    Target ORF information:

    RefSeq Version XM_021472147.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio diacylglycerol kinase, eta (dgkh), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021472147.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGACGGCCA AGAGAACCGC TTTCCAGCCG TTGTCCAGCT CCCGCATCCC AGCGGAGAGG 
    AAGGGAGCGG CTGAGCGCGT TCCTAACCCG GAGTGCTGTG ACTTTCCCCC GCAGGAGCCG
    GAGAAAGCAG CTGCGTCCGC CTCGGGGGTT CCCGCCGTGG GGGCTGTCGG GGGCTCGGGG
    CCGGTGGTGG ACGAGTCATC CGACAGCGAA GGAGAGCAGG AAGGGCCGCA GAAACTCATC
    AGGAAAGTGT CAACCTCCGG GCAAATACGA AGCAAGACAA GCGTGAAGGA AGGGCTTCTT
    CTGAAACAGA CAAGCTCGTT CCAGCGTTGG AAAAAACGTT ATTTCAAGCT ACGAGGACGG
    ACGCTCTACT ATGCTAAGGA TGCCAAGGCG GGTGGCGTTT TGGCCCGCTG GCAGTATCGG
    GTCTCTCGTA GCCGCGTGTG CTGGAGGGTG CTTTCCGTGT GCTGGACCGG TGCCCGCCGT
    CCGCCGCCCG CTCTTGGCGC CAGTGGATCG GAGGAAGGCT TGGTGGCGAT TCGGTCTCTT
    ATTTTCGATG AGGTGGACCT TTCAGATGCC AGTGTTGCCG AATCAAGTAC AAAGAATATC
    AACAACAGTT TCACGGTAAG AGGCCATTTC CAGGAATGCT GCAAATATCC ACAATATACA
    GCAAATATTA AGAGAGTACA TTATAAGTGT AACATTAACT ACAAGACCAC CAGATTCTAC
    AAGATCACGC TGTTCTCATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa145029
    Clone ID Related Accession (Same CDS sequence) XM_021472147.1 , XM_021474623.1
    Accession Version XM_021474623.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 741bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product diacylglycerol kinase eta isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395383.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGACGGCCA AGAGAACCGC TTTCCAGCCG TTGTCCAGCT CCCGCATCCC AGCGGAGAGG 
    AAGGGAGCGG CTGAGCGCGT TCCTAACCCG GAGTGCTGTG ACTTTCCCCC GCAGGAGCCG
    GAGAAAGCAG CTGCGTCCGC CTCGGGGGTT CCCGCCGTGG GGGCTGTCGG GGGCTCGGGG
    CCGGTGGTGG ACGAGTCATC CGACAGCGAA GGAGAGCAGG AAGGGCCGCA GAAACTCATC
    AGGAAAGTGT CAACCTCCGG GCAAATACGA AGCAAGACAA GCGTGAAGGA AGGGCTTCTT
    CTGAAACAGA CAAGCTCGTT CCAGCGTTGG AAAAAACGTT ATTTCAAGCT ACGAGGACGG
    ACGCTCTACT ATGCTAAGGA TGCCAAGGCG GGTGGCGTTT TGGCCCGCTG GCAGTATCGG
    GTCTCTCGTA GCCGCGTGTG CTGGAGGGTG CTTTCCGTGT GCTGGACCGG TGCCCGCCGT
    CCGCCGCCCG CTCTTGGCGC CAGTGGATCG GAGGAAGGCT TGGTGGCGAT TCGGTCTCTT
    ATTTTCGATG AGGTGGACCT TTCAGATGCC AGTGTTGCCG AATCAAGTAC AAAGAATATC
    AACAACAGTT TCACGGTAAG AGGCCATTTC CAGGAATGCT GCAAATATCC ACAATATACA
    GCAAATATTA AGAGAGTACA TTATAAGTGT AACATTAACT ACAAGACCAC CAGATTCTAC
    AAGATCACGC TGTTCTCATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330298.1
    CDS803..1543
    Translation

    Target ORF information:

    RefSeq Version XM_021474623.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio diacylglycerol kinase, eta (dgkh), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474623.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGACGGCCA AGAGAACCGC TTTCCAGCCG TTGTCCAGCT CCCGCATCCC AGCGGAGAGG 
    AAGGGAGCGG CTGAGCGCGT TCCTAACCCG GAGTGCTGTG ACTTTCCCCC GCAGGAGCCG
    GAGAAAGCAG CTGCGTCCGC CTCGGGGGTT CCCGCCGTGG GGGCTGTCGG GGGCTCGGGG
    CCGGTGGTGG ACGAGTCATC CGACAGCGAA GGAGAGCAGG AAGGGCCGCA GAAACTCATC
    AGGAAAGTGT CAACCTCCGG GCAAATACGA AGCAAGACAA GCGTGAAGGA AGGGCTTCTT
    CTGAAACAGA CAAGCTCGTT CCAGCGTTGG AAAAAACGTT ATTTCAAGCT ACGAGGACGG
    ACGCTCTACT ATGCTAAGGA TGCCAAGGCG GGTGGCGTTT TGGCCCGCTG GCAGTATCGG
    GTCTCTCGTA GCCGCGTGTG CTGGAGGGTG CTTTCCGTGT GCTGGACCGG TGCCCGCCGT
    CCGCCGCCCG CTCTTGGCGC CAGTGGATCG GAGGAAGGCT TGGTGGCGAT TCGGTCTCTT
    ATTTTCGATG AGGTGGACCT TTCAGATGCC AGTGTTGCCG AATCAAGTAC AAAGAATATC
    AACAACAGTT TCACGGTAAG AGGCCATTTC CAGGAATGCT GCAAATATCC ACAATATACA
    GCAAATATTA AGAGAGTACA TTATAAGTGT AACATTAACT ACAAGACCAC CAGATTCTAC
    AAGATCACGC TGTTCTCATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa145030
    Clone ID Related Accession (Same CDS sequence) XM_021472148.1 , XM_021474624.1
    Accession Version XM_021472148.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 675bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product diacylglycerol kinase eta isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394746.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGGAAGATG ACTGCCAATA TACTCGCAGC CCTGCCTGGG AAGAACTGGA GCCGGAGAAA 
    GCAGCTGCGT CCGCCTCGGG GGTTCCCGCC GTGGGGGCTG TCGGGGGCTC GGGGCCGGTG
    GTGGACGAGT CATCCGACAG CGAAGGAGAG CAGGAAGGGC CGCAGAAACT CATCAGGAAA
    GTGTCAACCT CCGGGCAAAT ACGAAGCAAG ACAAGCGTGA AGGAAGGGCT TCTTCTGAAA
    CAGACAAGCT CGTTCCAGCG TTGGAAAAAA CGTTATTTCA AGCTACGAGG ACGGACGCTC
    TACTATGCTA AGGATGCCAA GGCGGGTGGC GTTTTGGCCC GCTGGCAGTA TCGGGTCTCT
    CGTAGCCGCG TGTGCTGGAG GGTGCTTTCC GTGTGCTGGA CCGGTGCCCG CCGTCCGCCG
    CCCGCTCTTG GCGCCAGTGG ATCGGAGGAA GGCTTGGTGG CGATTCGGTC TCTTATTTTC
    GATGAGGTGG ACCTTTCAGA TGCCAGTGTT GCCGAATCAA GTACAAAGAA TATCAACAAC
    AGTTTCACGG TAAGAGGCCA TTTCCAGGAA TGCTGCAAAT ATCCACAATA TACAGCAAAT
    ATTAAGAGAG TACATTATAA GTGTAACATT AACTACAAGA CCACCAGATT CTACAAGATC
    ACGCTGTTCT CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327823.1
    CDS381..1055
    Translation

    Target ORF information:

    RefSeq Version XM_021472148.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio diacylglycerol kinase, eta (dgkh), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021472148.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGGAAGATG ACTGCCAATA TACTCGCAGC CCTGCCTGGG AAGAACTGGA GCCGGAGAAA 
    GCAGCTGCGT CCGCCTCGGG GGTTCCCGCC GTGGGGGCTG TCGGGGGCTC GGGGCCGGTG
    GTGGACGAGT CATCCGACAG CGAAGGAGAG CAGGAAGGGC CGCAGAAACT CATCAGGAAA
    GTGTCAACCT CCGGGCAAAT ACGAAGCAAG ACAAGCGTGA AGGAAGGGCT TCTTCTGAAA
    CAGACAAGCT CGTTCCAGCG TTGGAAAAAA CGTTATTTCA AGCTACGAGG ACGGACGCTC
    TACTATGCTA AGGATGCCAA GGCGGGTGGC GTTTTGGCCC GCTGGCAGTA TCGGGTCTCT
    CGTAGCCGCG TGTGCTGGAG GGTGCTTTCC GTGTGCTGGA CCGGTGCCCG CCGTCCGCCG
    CCCGCTCTTG GCGCCAGTGG ATCGGAGGAA GGCTTGGTGG CGATTCGGTC TCTTATTTTC
    GATGAGGTGG ACCTTTCAGA TGCCAGTGTT GCCGAATCAA GTACAAAGAA TATCAACAAC
    AGTTTCACGG TAAGAGGCCA TTTCCAGGAA TGCTGCAAAT ATCCACAATA TACAGCAAAT
    ATTAAGAGAG TACATTATAA GTGTAACATT AACTACAAGA CCACCAGATT CTACAAGATC
    ACGCTGTTCT CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa145030
    Clone ID Related Accession (Same CDS sequence) XM_021472148.1 , XM_021474624.1
    Accession Version XM_021474624.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 675bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product diacylglycerol kinase eta isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395383.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGGAAGATG ACTGCCAATA TACTCGCAGC CCTGCCTGGG AAGAACTGGA GCCGGAGAAA 
    GCAGCTGCGT CCGCCTCGGG GGTTCCCGCC GTGGGGGCTG TCGGGGGCTC GGGGCCGGTG
    GTGGACGAGT CATCCGACAG CGAAGGAGAG CAGGAAGGGC CGCAGAAACT CATCAGGAAA
    GTGTCAACCT CCGGGCAAAT ACGAAGCAAG ACAAGCGTGA AGGAAGGGCT TCTTCTGAAA
    CAGACAAGCT CGTTCCAGCG TTGGAAAAAA CGTTATTTCA AGCTACGAGG ACGGACGCTC
    TACTATGCTA AGGATGCCAA GGCGGGTGGC GTTTTGGCCC GCTGGCAGTA TCGGGTCTCT
    CGTAGCCGCG TGTGCTGGAG GGTGCTTTCC GTGTGCTGGA CCGGTGCCCG CCGTCCGCCG
    CCCGCTCTTG GCGCCAGTGG ATCGGAGGAA GGCTTGGTGG CGATTCGGTC TCTTATTTTC
    GATGAGGTGG ACCTTTCAGA TGCCAGTGTT GCCGAATCAA GTACAAAGAA TATCAACAAC
    AGTTTCACGG TAAGAGGCCA TTTCCAGGAA TGCTGCAAAT ATCCACAATA TACAGCAAAT
    ATTAAGAGAG TACATTATAA GTGTAACATT AACTACAAGA CCACCAGATT CTACAAGATC
    ACGCTGTTCT CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330299.1
    CDS381..1055
    Translation

    Target ORF information:

    RefSeq Version XM_021474624.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio diacylglycerol kinase, eta (dgkh), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474624.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGGAAGATG ACTGCCAATA TACTCGCAGC CCTGCCTGGG AAGAACTGGA GCCGGAGAAA 
    GCAGCTGCGT CCGCCTCGGG GGTTCCCGCC GTGGGGGCTG TCGGGGGCTC GGGGCCGGTG
    GTGGACGAGT CATCCGACAG CGAAGGAGAG CAGGAAGGGC CGCAGAAACT CATCAGGAAA
    GTGTCAACCT CCGGGCAAAT ACGAAGCAAG ACAAGCGTGA AGGAAGGGCT TCTTCTGAAA
    CAGACAAGCT CGTTCCAGCG TTGGAAAAAA CGTTATTTCA AGCTACGAGG ACGGACGCTC
    TACTATGCTA AGGATGCCAA GGCGGGTGGC GTTTTGGCCC GCTGGCAGTA TCGGGTCTCT
    CGTAGCCGCG TGTGCTGGAG GGTGCTTTCC GTGTGCTGGA CCGGTGCCCG CCGTCCGCCG
    CCCGCTCTTG GCGCCAGTGG ATCGGAGGAA GGCTTGGTGG CGATTCGGTC TCTTATTTTC
    GATGAGGTGG ACCTTTCAGA TGCCAGTGTT GCCGAATCAA GTACAAAGAA TATCAACAAC
    AGTTTCACGG TAAGAGGCCA TTTCCAGGAA TGCTGCAAAT ATCCACAATA TACAGCAAAT
    ATTAAGAGAG TACATTATAA GTGTAACATT AACTACAAGA CCACCAGATT CTACAAGATC
    ACGCTGTTCT CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa145031
    Clone ID Related Accession (Same CDS sequence) XM_021472149.1 , XM_021474625.1
    Accession Version XM_021472149.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 594bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product diacylglycerol kinase eta isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394746.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGACGGCCA AGAGAACCGC TTTCCAGCCG TTGTCCAGCT CCCGCATCCC AGCGGAGAGG 
    AAGGGAGCGG CTGAGCGCGT TCCTAACCCG GAGTGCTGTG ACTTTCCCCC GCAGGAGCCG
    GAGAAAGCAG CTGCGTCCGC CTCGGGGGTT CCCGCCGTGG GGGCTGTCGG GGGCTCGGGG
    CCGGTGGTGG ACGAGTCATC CGACAGCGAA GGAGAGCAGG AAGGGCCGCA GAAACTCATC
    AGGAAAGTGT CAACCTCCGG GCAAATACGA AGCAAGACAA GCGTGAAGGA AGGGCTTCTT
    CTGAAACAGA CAAGCTCGTT CCAGCGTTGG AAAAAACGTT ATTTCAAGCT ACGAGGACGG
    ACGCTCTACT ATGCTAAGGA TGCCAAGTCT CTTATTTTCG ATGAGGTGGA CCTTTCAGAT
    GCCAGTGTTG CCGAATCAAG TACAAAGAAT ATCAACAACA GTTTCACGGT AAGAGGCCAT
    TTCCAGGAAT GCTGCAAATA TCCACAATAT ACAGCAAATA TTAAGAGAGT ACATTATAAG
    TGTAACATTA ACTACAAGAC CACCAGATTC TACAAGATCA CGCTGTTCTC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327824.1
    CDS803..1396
    Translation

    Target ORF information:

    RefSeq Version XM_021472149.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio diacylglycerol kinase, eta (dgkh), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021472149.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGACGGCCA AGAGAACCGC TTTCCAGCCG TTGTCCAGCT CCCGCATCCC AGCGGAGAGG 
    AAGGGAGCGG CTGAGCGCGT TCCTAACCCG GAGTGCTGTG ACTTTCCCCC GCAGGAGCCG
    GAGAAAGCAG CTGCGTCCGC CTCGGGGGTT CCCGCCGTGG GGGCTGTCGG GGGCTCGGGG
    CCGGTGGTGG ACGAGTCATC CGACAGCGAA GGAGAGCAGG AAGGGCCGCA GAAACTCATC
    AGGAAAGTGT CAACCTCCGG GCAAATACGA AGCAAGACAA GCGTGAAGGA AGGGCTTCTT
    CTGAAACAGA CAAGCTCGTT CCAGCGTTGG AAAAAACGTT ATTTCAAGCT ACGAGGACGG
    ACGCTCTACT ATGCTAAGGA TGCCAAGTCT CTTATTTTCG ATGAGGTGGA CCTTTCAGAT
    GCCAGTGTTG CCGAATCAAG TACAAAGAAT ATCAACAACA GTTTCACGGT AAGAGGCCAT
    TTCCAGGAAT GCTGCAAATA TCCACAATAT ACAGCAAATA TTAAGAGAGT ACATTATAAG
    TGTAACATTA ACTACAAGAC CACCAGATTC TACAAGATCA CGCTGTTCTC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa145031
    Clone ID Related Accession (Same CDS sequence) XM_021472149.1 , XM_021474625.1
    Accession Version XM_021474625.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 594bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product diacylglycerol kinase eta isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395383.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGACGGCCA AGAGAACCGC TTTCCAGCCG TTGTCCAGCT CCCGCATCCC AGCGGAGAGG 
    AAGGGAGCGG CTGAGCGCGT TCCTAACCCG GAGTGCTGTG ACTTTCCCCC GCAGGAGCCG
    GAGAAAGCAG CTGCGTCCGC CTCGGGGGTT CCCGCCGTGG GGGCTGTCGG GGGCTCGGGG
    CCGGTGGTGG ACGAGTCATC CGACAGCGAA GGAGAGCAGG AAGGGCCGCA GAAACTCATC
    AGGAAAGTGT CAACCTCCGG GCAAATACGA AGCAAGACAA GCGTGAAGGA AGGGCTTCTT
    CTGAAACAGA CAAGCTCGTT CCAGCGTTGG AAAAAACGTT ATTTCAAGCT ACGAGGACGG
    ACGCTCTACT ATGCTAAGGA TGCCAAGTCT CTTATTTTCG ATGAGGTGGA CCTTTCAGAT
    GCCAGTGTTG CCGAATCAAG TACAAAGAAT ATCAACAACA GTTTCACGGT AAGAGGCCAT
    TTCCAGGAAT GCTGCAAATA TCCACAATAT ACAGCAAATA TTAAGAGAGT ACATTATAAG
    TGTAACATTA ACTACAAGAC CACCAGATTC TACAAGATCA CGCTGTTCTC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330300.1
    CDS803..1396
    Translation

    Target ORF information:

    RefSeq Version XM_021474625.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio diacylglycerol kinase, eta (dgkh), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474625.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGACGGCCA AGAGAACCGC TTTCCAGCCG TTGTCCAGCT CCCGCATCCC AGCGGAGAGG 
    AAGGGAGCGG CTGAGCGCGT TCCTAACCCG GAGTGCTGTG ACTTTCCCCC GCAGGAGCCG
    GAGAAAGCAG CTGCGTCCGC CTCGGGGGTT CCCGCCGTGG GGGCTGTCGG GGGCTCGGGG
    CCGGTGGTGG ACGAGTCATC CGACAGCGAA GGAGAGCAGG AAGGGCCGCA GAAACTCATC
    AGGAAAGTGT CAACCTCCGG GCAAATACGA AGCAAGACAA GCGTGAAGGA AGGGCTTCTT
    CTGAAACAGA CAAGCTCGTT CCAGCGTTGG AAAAAACGTT ATTTCAAGCT ACGAGGACGG
    ACGCTCTACT ATGCTAAGGA TGCCAAGTCT CTTATTTTCG ATGAGGTGGA CCTTTCAGAT
    GCCAGTGTTG CCGAATCAAG TACAAAGAAT ATCAACAACA GTTTCACGGT AAGAGGCCAT
    TTCCAGGAAT GCTGCAAATA TCCACAATAT ACAGCAAATA TTAAGAGAGT ACATTATAAG
    TGTAACATTA ACTACAAGAC CACCAGATTC TACAAGATCA CGCTGTTCTC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa15020
    Clone ID Related Accession (Same CDS sequence) XM_009304586.2
    Accession Version XM_009304586.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3255bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product diacylglycerol kinase eta isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304586.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGAGGACT GGATCAGCTC GCTGAAATCG GTTCAGTCCA GAGAGCATTA CGAGACGGCA 
    CAGTTTAATG TGGAACATTT CTCAGGGATG CACAACTGGT ATGCCTGCTC CCACGCTCGC
    CCCACCTTCT GTAACGTCTG TCGGGACAGC CTCTCAGGGG TCACTTCTCA TGGCCTCTCC
    TGTGAAGTGT GCAAATTCAA AGCCCACAAA AGATGTGCAG TCAGGGCCAC TAACAACTGC
    AAATGGACAA CATTGGCCTC CATCGGGAAG GACATTATTG AAGACGAGGA TGGGATTGCA
    ATGCCGCACC AATGGCTGGA AGGCAACCTG CCGGTTAGTG CAAAGTGTGC TGTTTGCGAC
    AAAACATGTG GCAGCGTGCT GCGCTTGCAG GACTGGCGCT GTTTGTGGTG CAAGGCCATG
    GTGCACACGG CATGCATGGA TCTCTACCCC CGCAAATGTC CCCTCGGCCA GTGCAAAGTG
    TCCATCATCC CCCCCACTGC CCTTAACAGC ATCGACTCAG ACGGTTTTTG GAAGGCCACG
    TGCCCTCCAT CATGTGCAAG TCCGCTCCTC GTGTTTGTCA ACTCAAAAAG TGGAGACAAC
    CAAGGGGTAA AATTCCTGCG TCGCTTCAAA CAGCTGCTCA ATCCTGCTCA GGTCTTTGAC
    CTGGTCAACG GTGGACCTCA TTTAGGGCTT CGCTTGTTCC AAAAGTTTGA CAACTTCCGG
    ATCCTTGTGT GTGGTGGAGA TGGAAGCGTT GGCTGGGTGC TGTCTGAAAT CGATAAACTA
    AATCTTCATA AACAGTGCCA GCTGGGTGTT CTGCCGTTGG GCACCGGGAA TGACCTAGCA
    CGGGTGCTAG GTTGGGGGCC GTCATGTGAT GATGACACTC AGCTGCCTCA GATACTGGAG
    AAGCTGGAGC GAGCTAGCAC CAAAATGCTG GACAGGTGGA GTATAATGAC GTATGAGATT
    AAAATACCTC CCAAACACAG CTGTCCTGCT ACGCCTGAGG AGGCAGAGGA CGGCCAGTTA
    CAGATATCAG CTTATGAGGA TTCAGTGGCT GCTCATCTCA CAAAGATTCT GAACTCAGAT
    CAGCACTCAG TGGTCATATC TTCATCAAAA GTATTGTGTG AGACGGTGAA GGAATTTGTG
    TCAAGAGTTG GAAAGTGTTA CGAGAGAGGC AGCGACAGCT CAGAAGAATC TGATGCACTG
    GCTCTCAAAT GTGCAATGCT GAATGAGAAG CTGGAGTCAC TCCTGCAGAC GTTAAATTTG
    GAGGCTCAGG TCCTGTCGCC CACAGCCCTT ACCACACCAC CCATCGTGGA GGAGGAGGTG
    GAGGAGGAAG AGCTGGAGGA GGAAGACGAA GATCTGAGCG AAGAGTCTCT AACAGAGTTG
    AAGGAGAACG AGACGGAGAA AGGATCCGCT CATAAACTCT TCAGACCCAG AGAGCAGCTG
    GTGCTGAGGG CCAACAGCCT GAAGAAAGCC CTCAGACAGA TCATCGAGCA GGCTGAGAGA
    ATGGTGGATG AACAGAACGC ACACACAGAG GAGCAGGAGC TTCAGTCTCC TTCTGACTTC
    AGGAAGGACA GCGAAGAGGA AAACAAAGAC AACGAGAAGG ACGAGGACAC TAAAGAACTC
    GAAACTCTGC CTTTGGCAAA GTCTCCATGT TCTCCTCCAG AGAGACGCAT CAGTCGCAGC
    ACACAGTCCT GCGGATCCTT CTCCATCACT CCCTTCACCA CAAGCAAAGA GAACCTGCCC
    GTTCTCAACA CCCGCATCAT CTGTCCCGGT CTGCGGGCAG GTCTCGCTGC CTCCATCGCT
    GGAAGCTCTA TAATCAGTAA AATGCTGCTG GCCAACATTG ATCCGTTTGG TGCCACGCCA
    TTCATAGATC CTGACCTAGA TTCACTAGAG GGCTATTTGG AGAAGTGTGT GATGAATAAC
    TACTTTGGGA TCGGCTTGGA TGCAAAGATC TCACTGGAGT TCAACAACAA GCGTGAGGAG
    CATCCGGAGA AGTGCAGGAG TCGCACTAAA AACATGATGT GGTATGGTGT TCTGGGGACA
    AAAGAGTTGC TGCAGAGGAC TTATAAGAAC CTGGAACAGA AAGTTCAGCT GGAGTGTGAT
    GGCCAGTATA TCCCACTCCC AAGTCTCCAA GGAATTGCTG TGCTGAACAT CCCAAGCTAT
    GCTGGGGGAA CCAACTTCTG GGGCGGCACC AAAGAGGATG ATATATTTTG TGCCCCTTCA
    TTCGATGATA AAATTTTGGA AGTGGTCGCT GTGTTTGGTA GTATGCAGAT GGCTGTCTCA
    AGGGTGATTA AACTGCAGCA TCACCGTATT GCACAGTGTC GCACGGTGAA GATCACAATC
    CTGGGGGATG AAGGAGTGCC TGTCCAGGTG GATGGTGAAG CCTGGATTCA ACCTGCTGGA
    GTCATCAAGA TTCAGCACAA GAACCGGGCC CAGATGCTGA CAAGAGACCG GGCGTTTGAA
    AACACGTTGA AGTCTTGGGA GGATAAATTG AAATACGACA AGCCTCCGCT GCGGCCACAT
    CTGTACCCTC AGCAGTCTGT GGATCTGGCC ACAGAGGAGG AGGCCACGCT CATCCAGATG
    TGTGCCCGCG CTGCTGAGGA TCTCATCACC AGGATCTGTG AGGCTGCCAA AAACTACCAG
    CTCCTCGAGC AGGAGCTCGC CCACGCCGTC AACGCCTCCT CACATGCCAT TAACAAAACA
    CATCCCAAGT TCCCAGAGAG CCTGACAAGA AACACTGCCA TTGAGGTGGC CAGCACCGTG
    AAGGCCCTGC ACAATGAGAC AGAGTCTCTG TTAGTAGGAC GCGTGCCATT GCAACTGGAC
    CCTCCGCATG AAGATCTGCT GTCCACTGCG CTGCAGAACG TTGAGGTTGA ACTAGGCAAG
    CTGGTTGAGA TCCCTTGGCT CTACCACATC TTACAGCCCA ATGATGAGGA GGATCCCTCC
    CTTGAGTATG GAAAAAGAAA CAGTCGGAGT GGCATGTTCC GCATAGTGCC AAAGTTCAAG
    AAAGAGAAAG TGCCCAAGAA GTCTAGCCCA CAATCAGTTC AGAGGTGGGG TACTGAGGAG
    GTGGCCATCT GGCTGGAGCA GCTCAGCTTG GGAGAGTACA AAGACACCTT CATCCGCCAC
    GACATCCGCG GCTCCGAGCT CCTGCACCTG GAGAGGAGAG ACCTTAAGGA TCTGGGGATC
    TCGAAGGTGG GCCATATGAA GAGAATTCTC CAGGGAACTA AGGACTTGGC CAAGAGTTCG
    ATGGTGGACC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009302861.1
    CDS118..3372
    Translation

    Target ORF information:

    RefSeq Version XM_009304586.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio diacylglycerol kinase, eta (dgkh), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009304586.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGAGGACT GGATCAGCTC GCTGAAATCG GTTCAGTCCA GAGAGCATTA CGAGACGGCA 
    CAGTTTAATG TGGAACATTT CTCAGGGATG CACAACTGGT ATGCCTGCTC CCACGCTCGC
    CCCACCTTCT GTAACGTCTG TCGGGACAGC CTCTCAGGGG TCACTTCTCA TGGCCTCTCC
    TGTGAAGTGT GCAAATTCAA AGCCCACAAA AGATGTGCAG TCAGGGCCAC TAACAACTGC
    AAATGGACAA CATTGGCCTC CATCGGGAAG GACATTATTG AAGACGAGGA TGGGATTGCA
    ATGCCGCACC AATGGCTGGA AGGCAACCTG CCGGTTAGTG CAAAGTGTGC TGTTTGCGAC
    AAAACATGTG GCAGCGTGCT GCGCTTGCAG GACTGGCGCT GTTTGTGGTG CAAGGCCATG
    GTGCACACGG CATGCATGGA TCTCTACCCC CGCAAATGTC CCCTCGGCCA GTGCAAAGTG
    TCCATCATCC CCCCCACTGC CCTTAACAGC ATCGACTCAG ACGGTTTTTG GAAGGCCACG
    TGCCCTCCAT CATGTGCAAG TCCGCTCCTC GTGTTTGTCA ACTCAAAAAG TGGAGACAAC
    CAAGGGGTAA AATTCCTGCG TCGCTTCAAA CAGCTGCTCA ATCCTGCTCA GGTCTTTGAC
    CTGGTCAACG GTGGACCTCA TTTAGGGCTT CGCTTGTTCC AAAAGTTTGA CAACTTCCGG
    ATCCTTGTGT GTGGTGGAGA TGGAAGCGTT GGCTGGGTGC TGTCTGAAAT CGATAAACTA
    AATCTTCATA AACAGTGCCA GCTGGGTGTT CTGCCGTTGG GCACCGGGAA TGACCTAGCA
    CGGGTGCTAG GTTGGGGGCC GTCATGTGAT GATGACACTC AGCTGCCTCA GATACTGGAG
    AAGCTGGAGC GAGCTAGCAC CAAAATGCTG GACAGGTGGA GTATAATGAC GTATGAGATT
    AAAATACCTC CCAAACACAG CTGTCCTGCT ACGCCTGAGG AGGCAGAGGA CGGCCAGTTA
    CAGATATCAG CTTATGAGGA TTCAGTGGCT GCTCATCTCA CAAAGATTCT GAACTCAGAT
    CAGCACTCAG TGGTCATATC TTCATCAAAA GTATTGTGTG AGACGGTGAA GGAATTTGTG
    TCAAGAGTTG GAAAGTGTTA CGAGAGAGGC AGCGACAGCT CAGAAGAATC TGATGCACTG
    GCTCTCAAAT GTGCAATGCT GAATGAGAAG CTGGAGTCAC TCCTGCAGAC GTTAAATTTG
    GAGGCTCAGG TCCTGTCGCC CACAGCCCTT ACCACACCAC CCATCGTGGA GGAGGAGGTG
    GAGGAGGAAG AGCTGGAGGA GGAAGACGAA GATCTGAGCG AAGAGTCTCT AACAGAGTTG
    AAGGAGAACG AGACGGAGAA AGGATCCGCT CATAAACTCT TCAGACCCAG AGAGCAGCTG
    GTGCTGAGGG CCAACAGCCT GAAGAAAGCC CTCAGACAGA TCATCGAGCA GGCTGAGAGA
    ATGGTGGATG AACAGAACGC ACACACAGAG GAGCAGGAGC TTCAGTCTCC TTCTGACTTC
    AGGAAGGACA GCGAAGAGGA AAACAAAGAC AACGAGAAGG ACGAGGACAC TAAAGAACTC
    GAAACTCTGC CTTTGGCAAA GTCTCCATGT TCTCCTCCAG AGAGACGCAT CAGTCGCAGC
    ACACAGTCCT GCGGATCCTT CTCCATCACT CCCTTCACCA CAAGCAAAGA GAACCTGCCC
    GTTCTCAACA CCCGCATCAT CTGTCCCGGT CTGCGGGCAG GTCTCGCTGC CTCCATCGCT
    GGAAGCTCTA TAATCAGTAA AATGCTGCTG GCCAACATTG ATCCGTTTGG TGCCACGCCA
    TTCATAGATC CTGACCTAGA TTCACTAGAG GGCTATTTGG AGAAGTGTGT GATGAATAAC
    TACTTTGGGA TCGGCTTGGA TGCAAAGATC TCACTGGAGT TCAACAACAA GCGTGAGGAG
    CATCCGGAGA AGTGCAGGAG TCGCACTAAA AACATGATGT GGTATGGTGT TCTGGGGACA
    AAAGAGTTGC TGCAGAGGAC TTATAAGAAC CTGGAACAGA AAGTTCAGCT GGAGTGTGAT
    GGCCAGTATA TCCCACTCCC AAGTCTCCAA GGAATTGCTG TGCTGAACAT CCCAAGCTAT
    GCTGGGGGAA CCAACTTCTG GGGCGGCACC AAAGAGGATG ATATATTTTG TGCCCCTTCA
    TTCGATGATA AAATTTTGGA AGTGGTCGCT GTGTTTGGTA GTATGCAGAT GGCTGTCTCA
    AGGGTGATTA AACTGCAGCA TCACCGTATT GCACAGTGTC GCACGGTGAA GATCACAATC
    CTGGGGGATG AAGGAGTGCC TGTCCAGGTG GATGGTGAAG CCTGGATTCA ACCTGCTGGA
    GTCATCAAGA TTCAGCACAA GAACCGGGCC CAGATGCTGA CAAGAGACCG GGCGTTTGAA
    AACACGTTGA AGTCTTGGGA GGATAAATTG AAATACGACA AGCCTCCGCT GCGGCCACAT
    CTGTACCCTC AGCAGTCTGT GGATCTGGCC ACAGAGGAGG AGGCCACGCT CATCCAGATG
    TGTGCCCGCG CTGCTGAGGA TCTCATCACC AGGATCTGTG AGGCTGCCAA AAACTACCAG
    CTCCTCGAGC AGGAGCTCGC CCACGCCGTC AACGCCTCCT CACATGCCAT TAACAAAACA
    CATCCCAAGT TCCCAGAGAG CCTGACAAGA AACACTGCCA TTGAGGTGGC CAGCACCGTG
    AAGGCCCTGC ACAATGAGAC AGAGTCTCTG TTAGTAGGAC GCGTGCCATT GCAACTGGAC
    CCTCCGCATG AAGATCTGCT GTCCACTGCG CTGCAGAACG TTGAGGTTGA ACTAGGCAAG
    CTGGTTGAGA TCCCTTGGCT CTACCACATC TTACAGCCCA ATGATGAGGA GGATCCCTCC
    CTTGAGTATG GAAAAAGAAA CAGTCGGAGT GGCATGTTCC GCATAGTGCC AAAGTTCAAG
    AAAGAGAAAG TGCCCAAGAA GTCTAGCCCA CAATCAGTTC AGAGGTGGGG TACTGAGGAG
    GTGGCCATCT GGCTGGAGCA GCTCAGCTTG GGAGAGTACA AAGACACCTT CATCCGCCAC
    GACATCCGCG GCTCCGAGCT CCTGCACCTG GAGAGGAGAG ACCTTAAGGA TCTGGGGATC
    TCGAAGGTGG GCCATATGAA GAGAATTCTC CAGGGAACTA AGGACTTGGC CAAGAGTTCG
    ATGGTGGACC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48522
    Clone ID Related Accession (Same CDS sequence) XM_009304583.3 , XM_009304583.2
    Accession Version XM_009304583.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3921bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product diacylglycerol kinase eta isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304583.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGACGGCCA AGAGAACCGC TTTCCAGCCG TTGTCCAGCT CCCGCATCCC AGCGGAGAGG 
    AAGGGAGCGG CTGAGCGCGT TCCTAACCCG GAGTGCTGTG ACTTTCCCCC GCAGGAGCCG
    GAGAAAGCAG CTGCGTCCGC CTCGGGGGTT CCCGCCGTGG GGGCTGTCGG GGGCTCGGGG
    CCGGTGGTGG ACGAGTCATC CGACAGCGAA GGAGAGCAGG AAGGGCCGCA GAAACTCATC
    AGGAAAGTGT CAACCTCCGG GCAAATACGA AGCAAGACAA GCGTGAAGGA AGGGCTTCTT
    CTGAAACAGA CAAGCTCGTT CCAGCGTTGG AAAAAACGTT ATTTCAAGCT ACGAGGACGG
    ACGCTCTACT ATGCTAAGGA TGCCAAGGCG GGTGGCGTTT TGGCCCGCTG GCAGTATCGG
    GTCTCTCGTA GCCGCGTGTG CTGGAGGGTG CTTTCCGTGT GCTGGACCGG TGCCCGCCGT
    CCGCCGCCCG CTCTTGGCGC CAGTGGATCG GAGGAAGGCT TGGTGGCGAT TCGGTCTCTT
    ATTTTCGATG AGGTGGACCT TTCAGATGCC AGTGTTGCCG AATCAAGTAC AAAGAATATC
    AACAACAGTT TCACGGTAAT TACACCGTTT CGAAGGCTTA TCCTGTGCGC CGAGAACAGG
    AAAGAAATGG AGGACTGGAT CAGCTCGCTG AAATCGGTTC AGTCCAGAGA GCATTACGAG
    ACGGCACAGT TTAATGTGGA ACATTTCTCA GGGATGCACA ACTGGTATGC CTGCTCCCAC
    GCTCGCCCCA CCTTCTGTAA CGTCTGTCGG GACAGCCTCT CAGGGGTCAC TTCTCATGGC
    CTCTCCTGTG AAGTGTGCAA ATTCAAAGCC CACAAAAGAT GTGCAGTCAG GGCCACTAAC
    AACTGCAAAT GGACAACATT GGCCTCCATC GGGAAGGACA TTATTGAAGA CGAGGATGGG
    ATTGCAATGC CGCACCAATG GCTGGAAGGC AACCTGCCGG TTAGTGCAAA GTGTGCTGTT
    TGCGACAAAA CATGTGGCAG CGTGCTGCGC TTGCAGGACT GGCGCTGTTT GTGGTGCAAG
    GCCATGGTGC ACACGGCATG CATGGATCTC TACCCCCGCA AATGTCCCCT CGGCCAGTGC
    AAAGTGTCCA TCATCCCCCC CACTGCCCTT AACAGCATCG ACTCAGACGG TTTTTGGAAG
    GCCACGTGCC CTCCATCATG TGCAAGTCCG CTCCTCGTGT TTGTCAACTC AAAAAGTGGA
    GACAACCAAG GGGTAAAATT CCTGCGTCGC TTCAAACAGC TGCTCAATCC TGCTCAGGTC
    TTTGACCTGG TCAACGGTGG ACCTCATTTA GGGCTTCGCT TGTTCCAAAA GTTTGACAAC
    TTCCGGATCC TTGTGTGTGG TGGAGATGGA AGCGTTGGCT GGGTGCTGTC TGAAATCGAT
    AAACTAAATC TTCATAAACA GTGCCAGCTG GGTGTTCTGC CGTTGGGCAC CGGGAATGAC
    CTAGCACGGG TGCTAGGTTG GGGGCCGTCA TGTGATGATG ACACTCAGCT GCCTCAGATA
    CTGGAGAAGC TGGAGCGAGC TAGCACCAAA ATGCTGGACA GGTGGAGTAT AATGACGTAT
    GAGATTAAAA TACCTCCCAA ACACAGCTGT CCTGCTACGC CTGAGGAGGC AGAGGACGGC
    CAGTTACAGA TATCAGCTTA TGAGGATTCA GTGGCTGCTC ATCTCACAAA GATTCTGAAC
    TCAGATCAGC ACTCAGTGGT CATATCTTCA TCAAAAGTAT TGTGTGAGAC GGTGAAGGAA
    TTTGTGTCAA GAGTTGGAAA GTGTTACGAG AGAGGCAGCG ACAGCTCAGA AGAATCTGAT
    GCACTGGCTC TCAAATGTGC AATGCTGAAT GAGAAGCTGG AGTCACTCCT GCAGACGTTA
    AATTTGGAGG CTCAGGTCCT GTCGCCCACA GCCCTTACCA CACCACCCAT CGTGGAGGAG
    GAGGTGGAGG AGGAAGAGCT GGAGGAGGAA GACGAAGATC TGAGCGAAGA GTCTCTAACA
    GAGTTGAAGG AGAACGAGAC GGAGAAAGGA TCCGCTCATA AACTCTTCAG ACCCAGAGAG
    CAGCTGGTGC TGAGGGCCAA CAGCCTGAAG AAAGCCCTCA GACAGATCAT CGAGCAGGCT
    GAGAGAATGG TGGATGAACA GAACGCACAC ACAGAGGAGC AGGAGCTTCA GTCTCCTTCT
    GACTTCAGGA AGGACAGCGA AGAGGAAAAC AAAGACAACG AGAAGGACGA GGACACTAAA
    GAACTCGAAA CTCTGCCTTT GGCAAAGTCT CCATGTTCTC CTCCAGAGAG ACGCATCAGT
    CGCAGCACAC AGTCCTGCGG ATCCTTCTCC ATCACTCCCT TCACCACAAG CAAAGAGAAC
    CTGCCCGTTC TCAACACCCG CATCATCTGT CCCGGTCTGC GGGCAGGTCT CGCTGCCTCC
    ATCGCTGGAA GCTCTATAAT CAGTAAAATG CTGCTGGCCA ACATTGATCC GTTTGGTGCC
    ACGCCATTCA TAGATCCTGA CCTAGATTCA CTAGAGGGCT ATTTGGAGAA GTGTGTGATG
    AATAACTACT TTGGGATCGG CTTGGATGCA AAGATCTCAC TGGAGTTCAA CAACAAGCGT
    GAGGAGCATC CGGAGAAGTG CAGGAGTCGC ACTAAAAACA TGATGTGGTA TGGTGTTCTG
    GGGACAAAAG AGTTGCTGCA GAGGACTTAT AAGAACCTGG AACAGAAAGT TCAGCTGGAG
    TGTGATGGCC AGTATATCCC ACTCCCAAGT CTCCAAGGAA TTGCTGTGCT GAACATCCCA
    AGCTATGCTG GGGGAACCAA CTTCTGGGGC GGCACCAAAG AGGATGATAT ATTTTGTGCC
    CCTTCATTCG ATGATAAAAT TTTGGAAGTG GTCGCTGTGT TTGGTAGTAT GCAGATGGCT
    GTCTCAAGGG TGATTAAACT GCAGCATCAC CGTATTGCAC AGTGTCGCAC GGTGAAGATC
    ACAATCCTGG GGGATGAAGG AGTGCCTGTC CAGGTGGATG GTGAAGCCTG GATTCAACCT
    GCTGGAGTCA TCAAGATTCA GCACAAGAAC CGGGCCCAGA TGCTGACAAG AGACCGGGCG
    TTTGAAAACA CGTTGAAGTC TTGGGAGGAT AAATTGAAAT ACGACAAGCC TCCGCTGCGG
    CCACATCTGT ACCCTCAGCA GTCTGTGGAT CTGGCCACAG AGGAGGAGGC CACGCTCATC
    CAGATGTGTG CCCGCGCTGC TGAGGATCTC ATCACCAGGA TCTGTGAGGC TGCCAAAAAC
    TACCAGCTCC TCGAGCAGGA GCTCGCCCAC GCCGTCAACG CCTCCTCACA TGCCATTAAC
    AAAACACATC CCAAGTTCCC AGAGAGCCTG ACAAGAAACA CTGCCATTGA GGTGGCCAGC
    ACCGTGAAGG CCCTGCACAA TGAGACAGAG TCTCTGTTAG TAGGACGCGT GCCATTGCAA
    CTGGACCCTC CGCATGAAGA TCTGCTGTCC ACTGCGCTGC AGAACGTTGA GGTTGAACTA
    GGCAAGCTGG TTGAGATCCC TTGGCTCTAC CACATCTTAC AGCCCAATGA TGAGGAGGAT
    CCCTCCCTTG AGTATGGAAA AAGAAACAGT CGGAGTGGCA TGTTCCGCAT AGTGCCAAAG
    TTCAAGAAAG AGAAAGTGCC CAAGAAGTCT AGCCCACAAT CAGTTCAGAG GTGGGGTACT
    GAGGAGGTGG CCATCTGGCT GGAGCAGCTC AGCTTGGGAG AGTACAAAGA CACCTTCATC
    CGCCACGACA TCCGCGGCTC CGAGCTCCTG CACCTGGAGA GGAGAGACCT TAAGGATCTG
    GGGATCTCGA AGGTGGGCCA TATGAAGAGA ATTCTCCAGG GAACTAAGGA CTTGGCCAAG
    AGTTCGATGG TGGACCTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009302858.1
    CDS803..4723
    Translation

    Target ORF information:

    RefSeq Version XM_009304583.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio diacylglycerol kinase, eta (dgkh), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009304583.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGACGGCCA AGAGAACCGC TTTCCAGCCG TTGTCCAGCT CCCGCATCCC AGCGGAGAGG 
    AAGGGAGCGG CTGAGCGCGT TCCTAACCCG GAGTGCTGTG ACTTTCCCCC GCAGGAGCCG
    GAGAAAGCAG CTGCGTCCGC CTCGGGGGTT CCCGCCGTGG GGGCTGTCGG GGGCTCGGGG
    CCGGTGGTGG ACGAGTCATC CGACAGCGAA GGAGAGCAGG AAGGGCCGCA GAAACTCATC
    AGGAAAGTGT CAACCTCCGG GCAAATACGA AGCAAGACAA GCGTGAAGGA AGGGCTTCTT
    CTGAAACAGA CAAGCTCGTT CCAGCGTTGG AAAAAACGTT ATTTCAAGCT ACGAGGACGG
    ACGCTCTACT ATGCTAAGGA TGCCAAGGCG GGTGGCGTTT TGGCCCGCTG GCAGTATCGG
    GTCTCTCGTA GCCGCGTGTG CTGGAGGGTG CTTTCCGTGT GCTGGACCGG TGCCCGCCGT
    CCGCCGCCCG CTCTTGGCGC CAGTGGATCG GAGGAAGGCT TGGTGGCGAT TCGGTCTCTT
    ATTTTCGATG AGGTGGACCT TTCAGATGCC AGTGTTGCCG AATCAAGTAC AAAGAATATC
    AACAACAGTT TCACGGTAAT TACACCGTTT CGAAGGCTTA TCCTGTGCGC CGAGAACAGG
    AAAGAAATGG AGGACTGGAT CAGCTCGCTG AAATCGGTTC AGTCCAGAGA GCATTACGAG
    ACGGCACAGT TTAATGTGGA ACATTTCTCA GGGATGCACA ACTGGTATGC CTGCTCCCAC
    GCTCGCCCCA CCTTCTGTAA CGTCTGTCGG GACAGCCTCT CAGGGGTCAC TTCTCATGGC
    CTCTCCTGTG AAGTGTGCAA ATTCAAAGCC CACAAAAGAT GTGCAGTCAG GGCCACTAAC
    AACTGCAAAT GGACAACATT GGCCTCCATC GGGAAGGACA TTATTGAAGA CGAGGATGGG
    ATTGCAATGC CGCACCAATG GCTGGAAGGC AACCTGCCGG TTAGTGCAAA GTGTGCTGTT
    TGCGACAAAA CATGTGGCAG CGTGCTGCGC TTGCAGGACT GGCGCTGTTT GTGGTGCAAG
    GCCATGGTGC ACACGGCATG CATGGATCTC TACCCCCGCA AATGTCCCCT CGGCCAGTGC
    AAAGTGTCCA TCATCCCCCC CACTGCCCTT AACAGCATCG ACTCAGACGG TTTTTGGAAG
    GCCACGTGCC CTCCATCATG TGCAAGTCCG CTCCTCGTGT TTGTCAACTC AAAAAGTGGA
    GACAACCAAG GGGTAAAATT CCTGCGTCGC TTCAAACAGC TGCTCAATCC TGCTCAGGTC
    TTTGACCTGG TCAACGGTGG ACCTCATTTA GGGCTTCGCT TGTTCCAAAA GTTTGACAAC
    TTCCGGATCC TTGTGTGTGG TGGAGATGGA AGCGTTGGCT GGGTGCTGTC TGAAATCGAT
    AAACTAAATC TTCATAAACA GTGCCAGCTG GGTGTTCTGC CGTTGGGCAC CGGGAATGAC
    CTAGCACGGG TGCTAGGTTG GGGGCCGTCA TGTGATGATG ACACTCAGCT GCCTCAGATA
    CTGGAGAAGC TGGAGCGAGC TAGCACCAAA ATGCTGGACA GGTGGAGTAT AATGACGTAT
    GAGATTAAAA TACCTCCCAA ACACAGCTGT CCTGCTACGC CTGAGGAGGC AGAGGACGGC
    CAGTTACAGA TATCAGCTTA TGAGGATTCA GTGGCTGCTC ATCTCACAAA GATTCTGAAC
    TCAGATCAGC ACTCAGTGGT CATATCTTCA TCAAAAGTAT TGTGTGAGAC GGTGAAGGAA
    TTTGTGTCAA GAGTTGGAAA GTGTTACGAG AGAGGCAGCG ACAGCTCAGA AGAATCTGAT
    GCACTGGCTC TCAAATGTGC AATGCTGAAT GAGAAGCTGG AGTCACTCCT GCAGACGTTA
    AATTTGGAGG CTCAGGTCCT GTCGCCCACA GCCCTTACCA CACCACCCAT CGTGGAGGAG
    GAGGTGGAGG AGGAAGAGCT GGAGGAGGAA GACGAAGATC TGAGCGAAGA GTCTCTAACA
    GAGTTGAAGG AGAACGAGAC GGAGAAAGGA TCCGCTCATA AACTCTTCAG ACCCAGAGAG
    CAGCTGGTGC TGAGGGCCAA CAGCCTGAAG AAAGCCCTCA GACAGATCAT CGAGCAGGCT
    GAGAGAATGG TGGATGAACA GAACGCACAC ACAGAGGAGC AGGAGCTTCA GTCTCCTTCT
    GACTTCAGGA AGGACAGCGA AGAGGAAAAC AAAGACAACG AGAAGGACGA GGACACTAAA
    GAACTCGAAA CTCTGCCTTT GGCAAAGTCT CCATGTTCTC CTCCAGAGAG ACGCATCAGT
    CGCAGCACAC AGTCCTGCGG ATCCTTCTCC ATCACTCCCT TCACCACAAG CAAAGAGAAC
    CTGCCCGTTC TCAACACCCG CATCATCTGT CCCGGTCTGC GGGCAGGTCT CGCTGCCTCC
    ATCGCTGGAA GCTCTATAAT CAGTAAAATG CTGCTGGCCA ACATTGATCC GTTTGGTGCC
    ACGCCATTCA TAGATCCTGA CCTAGATTCA CTAGAGGGCT ATTTGGAGAA GTGTGTGATG
    AATAACTACT TTGGGATCGG CTTGGATGCA AAGATCTCAC TGGAGTTCAA CAACAAGCGT
    GAGGAGCATC CGGAGAAGTG CAGGAGTCGC ACTAAAAACA TGATGTGGTA TGGTGTTCTG
    GGGACAAAAG AGTTGCTGCA GAGGACTTAT AAGAACCTGG AACAGAAAGT TCAGCTGGAG
    TGTGATGGCC AGTATATCCC ACTCCCAAGT CTCCAAGGAA TTGCTGTGCT GAACATCCCA
    AGCTATGCTG GGGGAACCAA CTTCTGGGGC GGCACCAAAG AGGATGATAT ATTTTGTGCC
    CCTTCATTCG ATGATAAAAT TTTGGAAGTG GTCGCTGTGT TTGGTAGTAT GCAGATGGCT
    GTCTCAAGGG TGATTAAACT GCAGCATCAC CGTATTGCAC AGTGTCGCAC GGTGAAGATC
    ACAATCCTGG GGGATGAAGG AGTGCCTGTC CAGGTGGATG GTGAAGCCTG GATTCAACCT
    GCTGGAGTCA TCAAGATTCA GCACAAGAAC CGGGCCCAGA TGCTGACAAG AGACCGGGCG
    TTTGAAAACA CGTTGAAGTC TTGGGAGGAT AAATTGAAAT ACGACAAGCC TCCGCTGCGG
    CCACATCTGT ACCCTCAGCA GTCTGTGGAT CTGGCCACAG AGGAGGAGGC CACGCTCATC
    CAGATGTGTG CCCGCGCTGC TGAGGATCTC ATCACCAGGA TCTGTGAGGC TGCCAAAAAC
    TACCAGCTCC TCGAGCAGGA GCTCGCCCAC GCCGTCAACG CCTCCTCACA TGCCATTAAC
    AAAACACATC CCAAGTTCCC AGAGAGCCTG ACAAGAAACA CTGCCATTGA GGTGGCCAGC
    ACCGTGAAGG CCCTGCACAA TGAGACAGAG TCTCTGTTAG TAGGACGCGT GCCATTGCAA
    CTGGACCCTC CGCATGAAGA TCTGCTGTCC ACTGCGCTGC AGAACGTTGA GGTTGAACTA
    GGCAAGCTGG TTGAGATCCC TTGGCTCTAC CACATCTTAC AGCCCAATGA TGAGGAGGAT
    CCCTCCCTTG AGTATGGAAA AAGAAACAGT CGGAGTGGCA TGTTCCGCAT AGTGCCAAAG
    TTCAAGAAAG AGAAAGTGCC CAAGAAGTCT AGCCCACAAT CAGTTCAGAG GTGGGGTACT
    GAGGAGGTGG CCATCTGGCT GGAGCAGCTC AGCTTGGGAG AGTACAAAGA CACCTTCATC
    CGCCACGACA TCCGCGGCTC CGAGCTCCTG CACCTGGAGA GGAGAGACCT TAAGGATCTG
    GGGATCTCGA AGGTGGGCCA TATGAAGAGA ATTCTCCAGG GAACTAAGGA CTTGGCCAAG
    AGTTCGATGG TGGACCTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48522
    Clone ID Related Accession (Same CDS sequence) XM_009304583.3 , XM_009304583.2
    Accession Version XM_009304583.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3921bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product diacylglycerol kinase eta isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304583.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGACGGCCA AGAGAACCGC TTTCCAGCCG TTGTCCAGCT CCCGCATCCC AGCGGAGAGG 
    AAGGGAGCGG CTGAGCGCGT TCCTAACCCG GAGTGCTGTG ACTTTCCCCC GCAGGAGCCG
    GAGAAAGCAG CTGCGTCCGC CTCGGGGGTT CCCGCCGTGG GGGCTGTCGG GGGCTCGGGG
    CCGGTGGTGG ACGAGTCATC CGACAGCGAA GGAGAGCAGG AAGGGCCGCA GAAACTCATC
    AGGAAAGTGT CAACCTCCGG GCAAATACGA AGCAAGACAA GCGTGAAGGA AGGGCTTCTT
    CTGAAACAGA CAAGCTCGTT CCAGCGTTGG AAAAAACGTT ATTTCAAGCT ACGAGGACGG
    ACGCTCTACT ATGCTAAGGA TGCCAAGGCG GGTGGCGTTT TGGCCCGCTG GCAGTATCGG
    GTCTCTCGTA GCCGCGTGTG CTGGAGGGTG CTTTCCGTGT GCTGGACCGG TGCCCGCCGT
    CCGCCGCCCG CTCTTGGCGC CAGTGGATCG GAGGAAGGCT TGGTGGCGAT TCGGTCTCTT
    ATTTTCGATG AGGTGGACCT TTCAGATGCC AGTGTTGCCG AATCAAGTAC AAAGAATATC
    AACAACAGTT TCACGGTAAT TACACCGTTT CGAAGGCTTA TCCTGTGCGC CGAGAACAGG
    AAAGAAATGG AGGACTGGAT CAGCTCGCTG AAATCGGTTC AGTCCAGAGA GCATTACGAG
    ACGGCACAGT TTAATGTGGA ACATTTCTCA GGGATGCACA ACTGGTATGC CTGCTCCCAC
    GCTCGCCCCA CCTTCTGTAA CGTCTGTCGG GACAGCCTCT CAGGGGTCAC TTCTCATGGC
    CTCTCCTGTG AAGTGTGCAA ATTCAAAGCC CACAAAAGAT GTGCAGTCAG GGCCACTAAC
    AACTGCAAAT GGACAACATT GGCCTCCATC GGGAAGGACA TTATTGAAGA CGAGGATGGG
    ATTGCAATGC CGCACCAATG GCTGGAAGGC AACCTGCCGG TTAGTGCAAA GTGTGCTGTT
    TGCGACAAAA CATGTGGCAG CGTGCTGCGC TTGCAGGACT GGCGCTGTTT GTGGTGCAAG
    GCCATGGTGC ACACGGCATG CATGGATCTC TACCCCCGCA AATGTCCCCT CGGCCAGTGC
    AAAGTGTCCA TCATCCCCCC CACTGCCCTT AACAGCATCG ACTCAGACGG TTTTTGGAAG
    GCCACGTGCC CTCCATCATG TGCAAGTCCG CTCCTCGTGT TTGTCAACTC AAAAAGTGGA
    GACAACCAAG GGGTAAAATT CCTGCGTCGC TTCAAACAGC TGCTCAATCC TGCTCAGGTC
    TTTGACCTGG TCAACGGTGG ACCTCATTTA GGGCTTCGCT TGTTCCAAAA GTTTGACAAC
    TTCCGGATCC TTGTGTGTGG TGGAGATGGA AGCGTTGGCT GGGTGCTGTC TGAAATCGAT
    AAACTAAATC TTCATAAACA GTGCCAGCTG GGTGTTCTGC CGTTGGGCAC CGGGAATGAC
    CTAGCACGGG TGCTAGGTTG GGGGCCGTCA TGTGATGATG ACACTCAGCT GCCTCAGATA
    CTGGAGAAGC TGGAGCGAGC TAGCACCAAA ATGCTGGACA GGTGGAGTAT AATGACGTAT
    GAGATTAAAA TACCTCCCAA ACACAGCTGT CCTGCTACGC CTGAGGAGGC AGAGGACGGC
    CAGTTACAGA TATCAGCTTA TGAGGATTCA GTGGCTGCTC ATCTCACAAA GATTCTGAAC
    TCAGATCAGC ACTCAGTGGT CATATCTTCA TCAAAAGTAT TGTGTGAGAC GGTGAAGGAA
    TTTGTGTCAA GAGTTGGAAA GTGTTACGAG AGAGGCAGCG ACAGCTCAGA AGAATCTGAT
    GCACTGGCTC TCAAATGTGC AATGCTGAAT GAGAAGCTGG AGTCACTCCT GCAGACGTTA
    AATTTGGAGG CTCAGGTCCT GTCGCCCACA GCCCTTACCA CACCACCCAT CGTGGAGGAG
    GAGGTGGAGG AGGAAGAGCT GGAGGAGGAA GACGAAGATC TGAGCGAAGA GTCTCTAACA
    GAGTTGAAGG AGAACGAGAC GGAGAAAGGA TCCGCTCATA AACTCTTCAG ACCCAGAGAG
    CAGCTGGTGC TGAGGGCCAA CAGCCTGAAG AAAGCCCTCA GACAGATCAT CGAGCAGGCT
    GAGAGAATGG TGGATGAACA GAACGCACAC ACAGAGGAGC AGGAGCTTCA GTCTCCTTCT
    GACTTCAGGA AGGACAGCGA AGAGGAAAAC AAAGACAACG AGAAGGACGA GGACACTAAA
    GAACTCGAAA CTCTGCCTTT GGCAAAGTCT CCATGTTCTC CTCCAGAGAG ACGCATCAGT
    CGCAGCACAC AGTCCTGCGG ATCCTTCTCC ATCACTCCCT TCACCACAAG CAAAGAGAAC
    CTGCCCGTTC TCAACACCCG CATCATCTGT CCCGGTCTGC GGGCAGGTCT CGCTGCCTCC
    ATCGCTGGAA GCTCTATAAT CAGTAAAATG CTGCTGGCCA ACATTGATCC GTTTGGTGCC
    ACGCCATTCA TAGATCCTGA CCTAGATTCA CTAGAGGGCT ATTTGGAGAA GTGTGTGATG
    AATAACTACT TTGGGATCGG CTTGGATGCA AAGATCTCAC TGGAGTTCAA CAACAAGCGT
    GAGGAGCATC CGGAGAAGTG CAGGAGTCGC ACTAAAAACA TGATGTGGTA TGGTGTTCTG
    GGGACAAAAG AGTTGCTGCA GAGGACTTAT AAGAACCTGG AACAGAAAGT TCAGCTGGAG
    TGTGATGGCC AGTATATCCC ACTCCCAAGT CTCCAAGGAA TTGCTGTGCT GAACATCCCA
    AGCTATGCTG GGGGAACCAA CTTCTGGGGC GGCACCAAAG AGGATGATAT ATTTTGTGCC
    CCTTCATTCG ATGATAAAAT TTTGGAAGTG GTCGCTGTGT TTGGTAGTAT GCAGATGGCT
    GTCTCAAGGG TGATTAAACT GCAGCATCAC CGTATTGCAC AGTGTCGCAC GGTGAAGATC
    ACAATCCTGG GGGATGAAGG AGTGCCTGTC CAGGTGGATG GTGAAGCCTG GATTCAACCT
    GCTGGAGTCA TCAAGATTCA GCACAAGAAC CGGGCCCAGA TGCTGACAAG AGACCGGGCG
    TTTGAAAACA CGTTGAAGTC TTGGGAGGAT AAATTGAAAT ACGACAAGCC TCCGCTGCGG
    CCACATCTGT ACCCTCAGCA GTCTGTGGAT CTGGCCACAG AGGAGGAGGC CACGCTCATC
    CAGATGTGTG CCCGCGCTGC TGAGGATCTC ATCACCAGGA TCTGTGAGGC TGCCAAAAAC
    TACCAGCTCC TCGAGCAGGA GCTCGCCCAC GCCGTCAACG CCTCCTCACA TGCCATTAAC
    AAAACACATC CCAAGTTCCC AGAGAGCCTG ACAAGAAACA CTGCCATTGA GGTGGCCAGC
    ACCGTGAAGG CCCTGCACAA TGAGACAGAG TCTCTGTTAG TAGGACGCGT GCCATTGCAA
    CTGGACCCTC CGCATGAAGA TCTGCTGTCC ACTGCGCTGC AGAACGTTGA GGTTGAACTA
    GGCAAGCTGG TTGAGATCCC TTGGCTCTAC CACATCTTAC AGCCCAATGA TGAGGAGGAT
    CCCTCCCTTG AGTATGGAAA AAGAAACAGT CGGAGTGGCA TGTTCCGCAT AGTGCCAAAG
    TTCAAGAAAG AGAAAGTGCC CAAGAAGTCT AGCCCACAAT CAGTTCAGAG GTGGGGTACT
    GAGGAGGTGG CCATCTGGCT GGAGCAGCTC AGCTTGGGAG AGTACAAAGA CACCTTCATC
    CGCCACGACA TCCGCGGCTC CGAGCTCCTG CACCTGGAGA GGAGAGACCT TAAGGATCTG
    GGGATCTCGA AGGTGGGCCA TATGAAGAGA ATTCTCCAGG GAACTAAGGA CTTGGCCAAG
    AGTTCGATGG TGGACCTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009302858.1
    CDS649..4569
    Translation

    Target ORF information:

    RefSeq Version XM_009304583.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio diacylglycerol kinase, eta (dgkh), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009304583.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGACGGCCA AGAGAACCGC TTTCCAGCCG TTGTCCAGCT CCCGCATCCC AGCGGAGAGG 
    AAGGGAGCGG CTGAGCGCGT TCCTAACCCG GAGTGCTGTG ACTTTCCCCC GCAGGAGCCG
    GAGAAAGCAG CTGCGTCCGC CTCGGGGGTT CCCGCCGTGG GGGCTGTCGG GGGCTCGGGG
    CCGGTGGTGG ACGAGTCATC CGACAGCGAA GGAGAGCAGG AAGGGCCGCA GAAACTCATC
    AGGAAAGTGT CAACCTCCGG GCAAATACGA AGCAAGACAA GCGTGAAGGA AGGGCTTCTT
    CTGAAACAGA CAAGCTCGTT CCAGCGTTGG AAAAAACGTT ATTTCAAGCT ACGAGGACGG
    ACGCTCTACT ATGCTAAGGA TGCCAAGGCG GGTGGCGTTT TGGCCCGCTG GCAGTATCGG
    GTCTCTCGTA GCCGCGTGTG CTGGAGGGTG CTTTCCGTGT GCTGGACCGG TGCCCGCCGT
    CCGCCGCCCG CTCTTGGCGC CAGTGGATCG GAGGAAGGCT TGGTGGCGAT TCGGTCTCTT
    ATTTTCGATG AGGTGGACCT TTCAGATGCC AGTGTTGCCG AATCAAGTAC AAAGAATATC
    AACAACAGTT TCACGGTAAT TACACCGTTT CGAAGGCTTA TCCTGTGCGC CGAGAACAGG
    AAAGAAATGG AGGACTGGAT CAGCTCGCTG AAATCGGTTC AGTCCAGAGA GCATTACGAG
    ACGGCACAGT TTAATGTGGA ACATTTCTCA GGGATGCACA ACTGGTATGC CTGCTCCCAC
    GCTCGCCCCA CCTTCTGTAA CGTCTGTCGG GACAGCCTCT CAGGGGTCAC TTCTCATGGC
    CTCTCCTGTG AAGTGTGCAA ATTCAAAGCC CACAAAAGAT GTGCAGTCAG GGCCACTAAC
    AACTGCAAAT GGACAACATT GGCCTCCATC GGGAAGGACA TTATTGAAGA CGAGGATGGG
    ATTGCAATGC CGCACCAATG GCTGGAAGGC AACCTGCCGG TTAGTGCAAA GTGTGCTGTT
    TGCGACAAAA CATGTGGCAG CGTGCTGCGC TTGCAGGACT GGCGCTGTTT GTGGTGCAAG
    GCCATGGTGC ACACGGCATG CATGGATCTC TACCCCCGCA AATGTCCCCT CGGCCAGTGC
    AAAGTGTCCA TCATCCCCCC CACTGCCCTT AACAGCATCG ACTCAGACGG TTTTTGGAAG
    GCCACGTGCC CTCCATCATG TGCAAGTCCG CTCCTCGTGT TTGTCAACTC AAAAAGTGGA
    GACAACCAAG GGGTAAAATT CCTGCGTCGC TTCAAACAGC TGCTCAATCC TGCTCAGGTC
    TTTGACCTGG TCAACGGTGG ACCTCATTTA GGGCTTCGCT TGTTCCAAAA GTTTGACAAC
    TTCCGGATCC TTGTGTGTGG TGGAGATGGA AGCGTTGGCT GGGTGCTGTC TGAAATCGAT
    AAACTAAATC TTCATAAACA GTGCCAGCTG GGTGTTCTGC CGTTGGGCAC CGGGAATGAC
    CTAGCACGGG TGCTAGGTTG GGGGCCGTCA TGTGATGATG ACACTCAGCT GCCTCAGATA
    CTGGAGAAGC TGGAGCGAGC TAGCACCAAA ATGCTGGACA GGTGGAGTAT AATGACGTAT
    GAGATTAAAA TACCTCCCAA ACACAGCTGT CCTGCTACGC CTGAGGAGGC AGAGGACGGC
    CAGTTACAGA TATCAGCTTA TGAGGATTCA GTGGCTGCTC ATCTCACAAA GATTCTGAAC
    TCAGATCAGC ACTCAGTGGT CATATCTTCA TCAAAAGTAT TGTGTGAGAC GGTGAAGGAA
    TTTGTGTCAA GAGTTGGAAA GTGTTACGAG AGAGGCAGCG ACAGCTCAGA AGAATCTGAT
    GCACTGGCTC TCAAATGTGC AATGCTGAAT GAGAAGCTGG AGTCACTCCT GCAGACGTTA
    AATTTGGAGG CTCAGGTCCT GTCGCCCACA GCCCTTACCA CACCACCCAT CGTGGAGGAG
    GAGGTGGAGG AGGAAGAGCT GGAGGAGGAA GACGAAGATC TGAGCGAAGA GTCTCTAACA
    GAGTTGAAGG AGAACGAGAC GGAGAAAGGA TCCGCTCATA AACTCTTCAG ACCCAGAGAG
    CAGCTGGTGC TGAGGGCCAA CAGCCTGAAG AAAGCCCTCA GACAGATCAT CGAGCAGGCT
    GAGAGAATGG TGGATGAACA GAACGCACAC ACAGAGGAGC AGGAGCTTCA GTCTCCTTCT
    GACTTCAGGA AGGACAGCGA AGAGGAAAAC AAAGACAACG AGAAGGACGA GGACACTAAA
    GAACTCGAAA CTCTGCCTTT GGCAAAGTCT CCATGTTCTC CTCCAGAGAG ACGCATCAGT
    CGCAGCACAC AGTCCTGCGG ATCCTTCTCC ATCACTCCCT TCACCACAAG CAAAGAGAAC
    CTGCCCGTTC TCAACACCCG CATCATCTGT CCCGGTCTGC GGGCAGGTCT CGCTGCCTCC
    ATCGCTGGAA GCTCTATAAT CAGTAAAATG CTGCTGGCCA ACATTGATCC GTTTGGTGCC
    ACGCCATTCA TAGATCCTGA CCTAGATTCA CTAGAGGGCT ATTTGGAGAA GTGTGTGATG
    AATAACTACT TTGGGATCGG CTTGGATGCA AAGATCTCAC TGGAGTTCAA CAACAAGCGT
    GAGGAGCATC CGGAGAAGTG CAGGAGTCGC ACTAAAAACA TGATGTGGTA TGGTGTTCTG
    GGGACAAAAG AGTTGCTGCA GAGGACTTAT AAGAACCTGG AACAGAAAGT TCAGCTGGAG
    TGTGATGGCC AGTATATCCC ACTCCCAAGT CTCCAAGGAA TTGCTGTGCT GAACATCCCA
    AGCTATGCTG GGGGAACCAA CTTCTGGGGC GGCACCAAAG AGGATGATAT ATTTTGTGCC
    CCTTCATTCG ATGATAAAAT TTTGGAAGTG GTCGCTGTGT TTGGTAGTAT GCAGATGGCT
    GTCTCAAGGG TGATTAAACT GCAGCATCAC CGTATTGCAC AGTGTCGCAC GGTGAAGATC
    ACAATCCTGG GGGATGAAGG AGTGCCTGTC CAGGTGGATG GTGAAGCCTG GATTCAACCT
    GCTGGAGTCA TCAAGATTCA GCACAAGAAC CGGGCCCAGA TGCTGACAAG AGACCGGGCG
    TTTGAAAACA CGTTGAAGTC TTGGGAGGAT AAATTGAAAT ACGACAAGCC TCCGCTGCGG
    CCACATCTGT ACCCTCAGCA GTCTGTGGAT CTGGCCACAG AGGAGGAGGC CACGCTCATC
    CAGATGTGTG CCCGCGCTGC TGAGGATCTC ATCACCAGGA TCTGTGAGGC TGCCAAAAAC
    TACCAGCTCC TCGAGCAGGA GCTCGCCCAC GCCGTCAACG CCTCCTCACA TGCCATTAAC
    AAAACACATC CCAAGTTCCC AGAGAGCCTG ACAAGAAACA CTGCCATTGA GGTGGCCAGC
    ACCGTGAAGG CCCTGCACAA TGAGACAGAG TCTCTGTTAG TAGGACGCGT GCCATTGCAA
    CTGGACCCTC CGCATGAAGA TCTGCTGTCC ACTGCGCTGC AGAACGTTGA GGTTGAACTA
    GGCAAGCTGG TTGAGATCCC TTGGCTCTAC CACATCTTAC AGCCCAATGA TGAGGAGGAT
    CCCTCCCTTG AGTATGGAAA AAGAAACAGT CGGAGTGGCA TGTTCCGCAT AGTGCCAAAG
    TTCAAGAAAG AGAAAGTGCC CAAGAAGTCT AGCCCACAAT CAGTTCAGAG GTGGGGTACT
    GAGGAGGTGG CCATCTGGCT GGAGCAGCTC AGCTTGGGAG AGTACAAAGA CACCTTCATC
    CGCCACGACA TCCGCGGCTC CGAGCTCCTG CACCTGGAGA GGAGAGACCT TAAGGATCTG
    GGGATCTCGA AGGTGGGCCA TATGAAGAGA ATTCTCCAGG GAACTAAGGA CTTGGCCAAG
    AGTTCGATGG TGGACCTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48523
    Clone ID Related Accession (Same CDS sequence) XM_009304584.2
    Accession Version XM_009304584.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3855bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product diacylglycerol kinase eta isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304584.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAAGATG ACTGCCAATA TACTCGCAGC CCTGCCTGGG AAGAACTGGA GCCGGAGAAA 
    GCAGCTGCGT CCGCCTCGGG GGTTCCCGCC GTGGGGGCTG TCGGGGGCTC GGGGCCGGTG
    GTGGACGAGT CATCCGACAG CGAAGGAGAG CAGGAAGGGC CGCAGAAACT CATCAGGAAA
    GTGTCAACCT CCGGGCAAAT ACGAAGCAAG ACAAGCGTGA AGGAAGGGCT TCTTCTGAAA
    CAGACAAGCT CGTTCCAGCG TTGGAAAAAA CGTTATTTCA AGCTACGAGG ACGGACGCTC
    TACTATGCTA AGGATGCCAA GGCGGGTGGC GTTTTGGCCC GCTGGCAGTA TCGGGTCTCT
    CGTAGCCGCG TGTGCTGGAG GGTGCTTTCC GTGTGCTGGA CCGGTGCCCG CCGTCCGCCG
    CCCGCTCTTG GCGCCAGTGG ATCGGAGGAA GGCTTGGTGG CGATTCGGTC TCTTATTTTC
    GATGAGGTGG ACCTTTCAGA TGCCAGTGTT GCCGAATCAA GTACAAAGAA TATCAACAAC
    AGTTTCACGG TAATTACACC GTTTCGAAGG CTTATCCTGT GCGCCGAGAA CAGGAAAGAA
    ATGGAGGACT GGATCAGCTC GCTGAAATCG GTTCAGTCCA GAGAGCATTA CGAGACGGCA
    CAGTTTAATG TGGAACATTT CTCAGGGATG CACAACTGGT ATGCCTGCTC CCACGCTCGC
    CCCACCTTCT GTAACGTCTG TCGGGACAGC CTCTCAGGGG TCACTTCTCA TGGCCTCTCC
    TGTGAAGTGT GCAAATTCAA AGCCCACAAA AGATGTGCAG TCAGGGCCAC TAACAACTGC
    AAATGGACAA CATTGGCCTC CATCGGGAAG GACATTATTG AAGACGAGGA TGGGATTGCA
    ATGCCGCACC AATGGCTGGA AGGCAACCTG CCGGTTAGTG CAAAGTGTGC TGTTTGCGAC
    AAAACATGTG GCAGCGTGCT GCGCTTGCAG GACTGGCGCT GTTTGTGGTG CAAGGCCATG
    GTGCACACGG CATGCATGGA TCTCTACCCC CGCAAATGTC CCCTCGGCCA GTGCAAAGTG
    TCCATCATCC CCCCCACTGC CCTTAACAGC ATCGACTCAG ACGGTTTTTG GAAGGCCACG
    TGCCCTCCAT CATGTGCAAG TCCGCTCCTC GTGTTTGTCA ACTCAAAAAG TGGAGACAAC
    CAAGGGGTAA AATTCCTGCG TCGCTTCAAA CAGCTGCTCA ATCCTGCTCA GGTCTTTGAC
    CTGGTCAACG GTGGACCTCA TTTAGGGCTT CGCTTGTTCC AAAAGTTTGA CAACTTCCGG
    ATCCTTGTGT GTGGTGGAGA TGGAAGCGTT GGCTGGGTGC TGTCTGAAAT CGATAAACTA
    AATCTTCATA AACAGTGCCA GCTGGGTGTT CTGCCGTTGG GCACCGGGAA TGACCTAGCA
    CGGGTGCTAG GTTGGGGGCC GTCATGTGAT GATGACACTC AGCTGCCTCA GATACTGGAG
    AAGCTGGAGC GAGCTAGCAC CAAAATGCTG GACAGGTGGA GTATAATGAC GTATGAGATT
    AAAATACCTC CCAAACACAG CTGTCCTGCT ACGCCTGAGG AGGCAGAGGA CGGCCAGTTA
    CAGATATCAG CTTATGAGGA TTCAGTGGCT GCTCATCTCA CAAAGATTCT GAACTCAGAT
    CAGCACTCAG TGGTCATATC TTCATCAAAA GTATTGTGTG AGACGGTGAA GGAATTTGTG
    TCAAGAGTTG GAAAGTGTTA CGAGAGAGGC AGCGACAGCT CAGAAGAATC TGATGCACTG
    GCTCTCAAAT GTGCAATGCT GAATGAGAAG CTGGAGTCAC TCCTGCAGAC GTTAAATTTG
    GAGGCTCAGG TCCTGTCGCC CACAGCCCTT ACCACACCAC CCATCGTGGA GGAGGAGGTG
    GAGGAGGAAG AGCTGGAGGA GGAAGACGAA GATCTGAGCG AAGAGTCTCT AACAGAGTTG
    AAGGAGAACG AGACGGAGAA AGGATCCGCT CATAAACTCT TCAGACCCAG AGAGCAGCTG
    GTGCTGAGGG CCAACAGCCT GAAGAAAGCC CTCAGACAGA TCATCGAGCA GGCTGAGAGA
    ATGGTGGATG AACAGAACGC ACACACAGAG GAGCAGGAGC TTCAGTCTCC TTCTGACTTC
    AGGAAGGACA GCGAAGAGGA AAACAAAGAC AACGAGAAGG ACGAGGACAC TAAAGAACTC
    GAAACTCTGC CTTTGGCAAA GTCTCCATGT TCTCCTCCAG AGAGACGCAT CAGTCGCAGC
    ACACAGTCCT GCGGATCCTT CTCCATCACT CCCTTCACCA CAAGCAAAGA GAACCTGCCC
    GTTCTCAACA CCCGCATCAT CTGTCCCGGT CTGCGGGCAG GTCTCGCTGC CTCCATCGCT
    GGAAGCTCTA TAATCAGTAA AATGCTGCTG GCCAACATTG ATCCGTTTGG TGCCACGCCA
    TTCATAGATC CTGACCTAGA TTCACTAGAG GGCTATTTGG AGAAGTGTGT GATGAATAAC
    TACTTTGGGA TCGGCTTGGA TGCAAAGATC TCACTGGAGT TCAACAACAA GCGTGAGGAG
    CATCCGGAGA AGTGCAGGAG TCGCACTAAA AACATGATGT GGTATGGTGT TCTGGGGACA
    AAAGAGTTGC TGCAGAGGAC TTATAAGAAC CTGGAACAGA AAGTTCAGCT GGAGTGTGAT
    GGCCAGTATA TCCCACTCCC AAGTCTCCAA GGAATTGCTG TGCTGAACAT CCCAAGCTAT
    GCTGGGGGAA CCAACTTCTG GGGCGGCACC AAAGAGGATG ATATATTTTG TGCCCCTTCA
    TTCGATGATA AAATTTTGGA AGTGGTCGCT GTGTTTGGTA GTATGCAGAT GGCTGTCTCA
    AGGGTGATTA AACTGCAGCA TCACCGTATT GCACAGTGTC GCACGGTGAA GATCACAATC
    CTGGGGGATG AAGGAGTGCC TGTCCAGGTG GATGGTGAAG CCTGGATTCA ACCTGCTGGA
    GTCATCAAGA TTCAGCACAA GAACCGGGCC CAGATGCTGA CAAGAGACCG GGCGTTTGAA
    AACACGTTGA AGTCTTGGGA GGATAAATTG AAATACGACA AGCCTCCGCT GCGGCCACAT
    CTGTACCCTC AGCAGTCTGT GGATCTGGCC ACAGAGGAGG AGGCCACGCT CATCCAGATG
    TGTGCCCGCG CTGCTGAGGA TCTCATCACC AGGATCTGTG AGGCTGCCAA AAACTACCAG
    CTCCTCGAGC AGGAGCTCGC CCACGCCGTC AACGCCTCCT CACATGCCAT TAACAAAACA
    CATCCCAAGT TCCCAGAGAG CCTGACAAGA AACACTGCCA TTGAGGTGGC CAGCACCGTG
    AAGGCCCTGC ACAATGAGAC AGAGTCTCTG TTAGTAGGAC GCGTGCCATT GCAACTGGAC
    CCTCCGCATG AAGATCTGCT GTCCACTGCG CTGCAGAACG TTGAGGTTGA ACTAGGCAAG
    CTGGTTGAGA TCCCTTGGCT CTACCACATC TTACAGCCCA ATGATGAGGA GGATCCCTCC
    CTTGAGTATG GAAAAAGAAA CAGTCGGAGT GGCATGTTCC GCATAGTGCC AAAGTTCAAG
    AAAGAGAAAG TGCCCAAGAA GTCTAGCCCA CAATCAGTTC AGAGGTGGGG TACTGAGGAG
    GTGGCCATCT GGCTGGAGCA GCTCAGCTTG GGAGAGTACA AAGACACCTT CATCCGCCAC
    GACATCCGCG GCTCCGAGCT CCTGCACCTG GAGAGGAGAG ACCTTAAGGA TCTGGGGATC
    TCGAAGGTGG GCCATATGAA GAGAATTCTC CAGGGAACTA AGGACTTGGC CAAGAGTTCG
    ATGGTGGACC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009302859.1
    CDS261..4115
    Translation

    Target ORF information:

    RefSeq Version XM_009304584.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio diacylglycerol kinase, eta (dgkh), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009304584.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAAGATG ACTGCCAATA TACTCGCAGC CCTGCCTGGG AAGAACTGGA GCCGGAGAAA 
    GCAGCTGCGT CCGCCTCGGG GGTTCCCGCC GTGGGGGCTG TCGGGGGCTC GGGGCCGGTG
    GTGGACGAGT CATCCGACAG CGAAGGAGAG CAGGAAGGGC CGCAGAAACT CATCAGGAAA
    GTGTCAACCT CCGGGCAAAT ACGAAGCAAG ACAAGCGTGA AGGAAGGGCT TCTTCTGAAA
    CAGACAAGCT CGTTCCAGCG TTGGAAAAAA CGTTATTTCA AGCTACGAGG ACGGACGCTC
    TACTATGCTA AGGATGCCAA GGCGGGTGGC GTTTTGGCCC GCTGGCAGTA TCGGGTCTCT
    CGTAGCCGCG TGTGCTGGAG GGTGCTTTCC GTGTGCTGGA CCGGTGCCCG CCGTCCGCCG
    CCCGCTCTTG GCGCCAGTGG ATCGGAGGAA GGCTTGGTGG CGATTCGGTC TCTTATTTTC
    GATGAGGTGG ACCTTTCAGA TGCCAGTGTT GCCGAATCAA GTACAAAGAA TATCAACAAC
    AGTTTCACGG TAATTACACC GTTTCGAAGG CTTATCCTGT GCGCCGAGAA CAGGAAAGAA
    ATGGAGGACT GGATCAGCTC GCTGAAATCG GTTCAGTCCA GAGAGCATTA CGAGACGGCA
    CAGTTTAATG TGGAACATTT CTCAGGGATG CACAACTGGT ATGCCTGCTC CCACGCTCGC
    CCCACCTTCT GTAACGTCTG TCGGGACAGC CTCTCAGGGG TCACTTCTCA TGGCCTCTCC
    TGTGAAGTGT GCAAATTCAA AGCCCACAAA AGATGTGCAG TCAGGGCCAC TAACAACTGC
    AAATGGACAA CATTGGCCTC CATCGGGAAG GACATTATTG AAGACGAGGA TGGGATTGCA
    ATGCCGCACC AATGGCTGGA AGGCAACCTG CCGGTTAGTG CAAAGTGTGC TGTTTGCGAC
    AAAACATGTG GCAGCGTGCT GCGCTTGCAG GACTGGCGCT GTTTGTGGTG CAAGGCCATG
    GTGCACACGG CATGCATGGA TCTCTACCCC CGCAAATGTC CCCTCGGCCA GTGCAAAGTG
    TCCATCATCC CCCCCACTGC CCTTAACAGC ATCGACTCAG ACGGTTTTTG GAAGGCCACG
    TGCCCTCCAT CATGTGCAAG TCCGCTCCTC GTGTTTGTCA ACTCAAAAAG TGGAGACAAC
    CAAGGGGTAA AATTCCTGCG TCGCTTCAAA CAGCTGCTCA ATCCTGCTCA GGTCTTTGAC
    CTGGTCAACG GTGGACCTCA TTTAGGGCTT CGCTTGTTCC AAAAGTTTGA CAACTTCCGG
    ATCCTTGTGT GTGGTGGAGA TGGAAGCGTT GGCTGGGTGC TGTCTGAAAT CGATAAACTA
    AATCTTCATA AACAGTGCCA GCTGGGTGTT CTGCCGTTGG GCACCGGGAA TGACCTAGCA
    CGGGTGCTAG GTTGGGGGCC GTCATGTGAT GATGACACTC AGCTGCCTCA GATACTGGAG
    AAGCTGGAGC GAGCTAGCAC CAAAATGCTG GACAGGTGGA GTATAATGAC GTATGAGATT
    AAAATACCTC CCAAACACAG CTGTCCTGCT ACGCCTGAGG AGGCAGAGGA CGGCCAGTTA
    CAGATATCAG CTTATGAGGA TTCAGTGGCT GCTCATCTCA CAAAGATTCT GAACTCAGAT
    CAGCACTCAG TGGTCATATC TTCATCAAAA GTATTGTGTG AGACGGTGAA GGAATTTGTG
    TCAAGAGTTG GAAAGTGTTA CGAGAGAGGC AGCGACAGCT CAGAAGAATC TGATGCACTG
    GCTCTCAAAT GTGCAATGCT GAATGAGAAG CTGGAGTCAC TCCTGCAGAC GTTAAATTTG
    GAGGCTCAGG TCCTGTCGCC CACAGCCCTT ACCACACCAC CCATCGTGGA GGAGGAGGTG
    GAGGAGGAAG AGCTGGAGGA GGAAGACGAA GATCTGAGCG AAGAGTCTCT AACAGAGTTG
    AAGGAGAACG AGACGGAGAA AGGATCCGCT CATAAACTCT TCAGACCCAG AGAGCAGCTG
    GTGCTGAGGG CCAACAGCCT GAAGAAAGCC CTCAGACAGA TCATCGAGCA GGCTGAGAGA
    ATGGTGGATG AACAGAACGC ACACACAGAG GAGCAGGAGC TTCAGTCTCC TTCTGACTTC
    AGGAAGGACA GCGAAGAGGA AAACAAAGAC AACGAGAAGG ACGAGGACAC TAAAGAACTC
    GAAACTCTGC CTTTGGCAAA GTCTCCATGT TCTCCTCCAG AGAGACGCAT CAGTCGCAGC
    ACACAGTCCT GCGGATCCTT CTCCATCACT CCCTTCACCA CAAGCAAAGA GAACCTGCCC
    GTTCTCAACA CCCGCATCAT CTGTCCCGGT CTGCGGGCAG GTCTCGCTGC CTCCATCGCT
    GGAAGCTCTA TAATCAGTAA AATGCTGCTG GCCAACATTG ATCCGTTTGG TGCCACGCCA
    TTCATAGATC CTGACCTAGA TTCACTAGAG GGCTATTTGG AGAAGTGTGT GATGAATAAC
    TACTTTGGGA TCGGCTTGGA TGCAAAGATC TCACTGGAGT TCAACAACAA GCGTGAGGAG
    CATCCGGAGA AGTGCAGGAG TCGCACTAAA AACATGATGT GGTATGGTGT TCTGGGGACA
    AAAGAGTTGC TGCAGAGGAC TTATAAGAAC CTGGAACAGA AAGTTCAGCT GGAGTGTGAT
    GGCCAGTATA TCCCACTCCC AAGTCTCCAA GGAATTGCTG TGCTGAACAT CCCAAGCTAT
    GCTGGGGGAA CCAACTTCTG GGGCGGCACC AAAGAGGATG ATATATTTTG TGCCCCTTCA
    TTCGATGATA AAATTTTGGA AGTGGTCGCT GTGTTTGGTA GTATGCAGAT GGCTGTCTCA
    AGGGTGATTA AACTGCAGCA TCACCGTATT GCACAGTGTC GCACGGTGAA GATCACAATC
    CTGGGGGATG AAGGAGTGCC TGTCCAGGTG GATGGTGAAG CCTGGATTCA ACCTGCTGGA
    GTCATCAAGA TTCAGCACAA GAACCGGGCC CAGATGCTGA CAAGAGACCG GGCGTTTGAA
    AACACGTTGA AGTCTTGGGA GGATAAATTG AAATACGACA AGCCTCCGCT GCGGCCACAT
    CTGTACCCTC AGCAGTCTGT GGATCTGGCC ACAGAGGAGG AGGCCACGCT CATCCAGATG
    TGTGCCCGCG CTGCTGAGGA TCTCATCACC AGGATCTGTG AGGCTGCCAA AAACTACCAG
    CTCCTCGAGC AGGAGCTCGC CCACGCCGTC AACGCCTCCT CACATGCCAT TAACAAAACA
    CATCCCAAGT TCCCAGAGAG CCTGACAAGA AACACTGCCA TTGAGGTGGC CAGCACCGTG
    AAGGCCCTGC ACAATGAGAC AGAGTCTCTG TTAGTAGGAC GCGTGCCATT GCAACTGGAC
    CCTCCGCATG AAGATCTGCT GTCCACTGCG CTGCAGAACG TTGAGGTTGA ACTAGGCAAG
    CTGGTTGAGA TCCCTTGGCT CTACCACATC TTACAGCCCA ATGATGAGGA GGATCCCTCC
    CTTGAGTATG GAAAAAGAAA CAGTCGGAGT GGCATGTTCC GCATAGTGCC AAAGTTCAAG
    AAAGAGAAAG TGCCCAAGAA GTCTAGCCCA CAATCAGTTC AGAGGTGGGG TACTGAGGAG
    GTGGCCATCT GGCTGGAGCA GCTCAGCTTG GGAGAGTACA AAGACACCTT CATCCGCCAC
    GACATCCGCG GCTCCGAGCT CCTGCACCTG GAGAGGAGAG ACCTTAAGGA TCTGGGGATC
    TCGAAGGTGG GCCATATGAA GAGAATTCTC CAGGGAACTA AGGACTTGGC CAAGAGTTCG
    ATGGTGGACC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48524
    Clone ID Related Accession (Same CDS sequence) XM_009304585.2 , XM_009304585.3
    Accession Version XM_009304585.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3756bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product diacylglycerol kinase eta isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304585.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGACGGCCA AGAGAACCGC TTTCCAGCCG TTGTCCAGCT CCCGCATCCC AGCGGAGAGG 
    AAGGGAGCGG CTGAGCGCGT TCCTAACCCG GAGTGCTGTG ACTTTCCCCC GCAGGAGCCG
    GAGAAAGCAG CTGCGTCCGC CTCGGGGGTT CCCGCCGTGG GGGCTGTCGG GGGCTCGGGG
    CCGGTGGTGG ACGAGTCATC CGACAGCGAA GGAGAGCAGG AAGGGCCGCA GAAACTCATC
    AGGAAAGTGT CAACCTCCGG GCAAATACGA AGCAAGACAA GCGTGAAGGA AGGGCTTCTT
    CTGAAACAGA CAAGCTCGTT CCAGCGTTGG AAAAAACGTT ATTTCAAGCT ACGAGGACGG
    ACGCTCTACT ATGCTAAGGA TGCCAAGGCG GGTGGCGTTT TGGCCCGCTG GCAGTATCGG
    GTCTCTCGTA GCCGCGTGTG CTGGAGGGTG CTTTCCGTGT GCTGGACCGG TGCCCGCCGT
    CCGCCGCCCG CTCTTGGCGC CAGTGGATCG GAGGAAGGCT TGGTGGCGAT TCGGTCTCTT
    ATTTTCGATG AGGTGGACCT TTCAGATGCC AGTGTTGCCG AATCAAGTAC AAAGAATATC
    AACAACAGTT TCACGGTAAT TACACCGTTT CGAAGGCTTA TCCTGTGCGC CGAGAACAGG
    AAAGAAATGG AGGACTGGAT CAGCTCGCTG AAATCGGTTC AGTCCAGAGA GCATTACGAG
    ACGGCACAGT TTAATGTGGA ACATTTCTCA GGGATGCACA ACTGGTATGC CTGCTCCCAC
    GCTCGCCCCA CCTTCTGTAA CGTCTGTCGG GACAGCCTCT CAGGGGTCAC TTCTCATGGC
    CTCTCCTGTG AAGTGTGCAA ATTCAAAGCC CACAAAAGAT GTGCAGTCAG GGCCACTAAC
    AACTGCAAAT GGACAACATT GGCCTCCATC GGGAAGGACA TTATTGAAGA CGAGGATGGG
    ATTGCAATGC CGCACCAATG GCTGGAAGGC AACCTGCCGG TTAGTGCAAA GTGTGCTGTT
    TGCGACAAAA CATGTGGCAG CGTGCTGCGC TTGCAGGACT GGCGCTGTTT GTGGTGCAAG
    GCCATGGTGC ACACGGCATG CATGGATCTC TACCCCCGCA AATGTCCCCT CGGCCAGTGC
    AAAGTGTCCA TCATCCCCCC CACTGCCCTT AACAGCATCG ACTCAGACGG TTTTTGGAAG
    GCCACGTGCC CTCCATCATG TGCAAGTCCG CTCCTCGTGT TTGTCAACTC AAAAAGTGGA
    GACAACCAAG GGGTAAAATT CCTGCGTCGC TTCAAACAGC TGCTCAATCC TGCTCAGGTC
    TTTGACCTGG TCAACGGTGG ACCTCATTTA GGGCTTCGCT TGTTCCAAAA GTTTGACAAC
    TTCCGGATCC TTGTGTGTGG TGGAGATGGA AGCGTTGGCT GGGTGCTGTC TGAAATCGAT
    AAACTAAATC TTCATAAACA GTGCCAGCTG GGTGTTCTGC CGTTGGGCAC CGGGAATGAC
    CTAGCACGGG TGCTAGGTTG GGGGCCGTCA TGTGATGATG ACACTCAGCT GCCTCAGATA
    CTGGAGAAGC TGGAGCGAGC TAGCACCAAA ATGCTGGACA GGTGGAGTAT AATGACGTAT
    GAGATTAAAA TACCTCCCAA ACACAGCTGT CCTGCTACGC CTGAGGAGGC AGAGGACGGC
    CAGTTACAGA TATCAGCTTA TGAGGATTCA GTGGCTGCTC ATCTCACAAA GATTCTGAAC
    TCAGATCAGC ACTCAGTGGT CATATCTTCA TCAAAAGTAT TGTGTGAGAC GGTGAAGGAA
    TTTGTGTCAA GAGTTGGAAA GTGTTACGAG AGAGGCAGCG ACAGCTCAGA AGAATCTGAT
    GCACTGGCTC TCAAATGTGC AATGCTGAAT GAGAAGCTGG AGTCACTCCT GCAGACGTTA
    AATTTGGAGG CTCAGGTCCT GTCGCCCACA GCCCTTACCA CACCACCCAT CGTGGAGGAG
    GAGGTGGAGG AGGAAGAGCT GGAGGAGGAA GACGAAGATC TGAGCGAAGA GTCTCTAACA
    GAGTTGAAGG AGAACGAGAC GGAGAAAGGA TCCGCTCATA AACTCTTCAG ACCCAGAGAG
    CAGCTGGTGC TGAGGGCCAA CAGCCTGAAG AAAGCCCTCA GACAGATCAT CGAGCAGGCT
    GAGAGAATGG TGGATGAACA GAACGCACAC ACAGAGGAGC AGGAGCTTCA GTCTCCTTCT
    GACTTCAGGA AGGACAGCGA AGAGGAAAAC AAAGACAACG AGAAGGACGA GGACACTAAA
    GAACTCGAAA CTCTGCCTTT GGCAAAGTCT CCATGTTCTC CTCCAGAGAG ACGCATCAGT
    CGCAGCACAC AGTCCTGCGG ATCCTTCTCC ATCACTCCCT TCACCACAAG CAAAGAGAAC
    CTGCCCGTTC TCAACACCCG CATCATCTGT CCCGGTCTGC GGGCAGGTCT CGCTGCCTCC
    ATCGCTGGAA GCTCTATAAT CAGTAAAATG CTGCTGGCCA ACATTGATCC GTTTGGTGCC
    ACGCCATTCA TAGATCCTGA CCTAGATTCA CTAGAGGGCT ATTTGGAGAA GTGTGTGATG
    AATAACTACT TTGGGATCGG CTTGGATGCA AAGATCTCAC TGGAGTTCAA CAACAAGCGT
    GAGGAGCATC CGGAGAAGTG CAGGAGTCGC ACTAAAAACA TGATGTGGTA TGGTGTTCTG
    GGGACAAAAG AGTTGCTGCA GAGGACTTAT AAGAACCTGG AACAGAAAGT TCAGCTGGAG
    TGTGATGGCC AGTATATCCC ACTCCCAAGT CTCCAAGGAA TTGCTGTGCT GAACATCCCA
    AGCTATGCTG GGGGAACCAA CTTCTGGGGC GGCACCAAAG AGGATGATAT ATTTTGTGCC
    CCTTCATTCG ATGATAAAAT TTTGGAAGTG GTCGCTGTGT TTGGTAGTAT GCAGATGGCT
    GTCTCAAGGG TGATTAAACT GCAGCATCAC CGTATTGCAC AGTGTCGCAC GGTGAAGATC
    ACAATCCTGG GGGATGAAGG AGTGCCTGTC CAGGTGGATG GTGAAGCCTG GATTCAACCT
    GCTGGAGTCA TCAAGATTCA GCACAAGAAC CGGGCCCAGA TGCTGACAAG AGACCGGGCG
    TTTGAAAACA CGTTGAAGTC TTGGGAGGAT AAATTGAAAT ACGACAAGCC TCCGCTGCGG
    CCACATCTGT ACCCTCAGCA GTCTGTGGAT CTGGCCACAG AGGAGGAGGC CACGCTCATC
    CAGATGTGTG CCCGCGCTGC TGAGGATCTC ATCACCAGGA TCTGTGAGGC TGCCAAAAAC
    TACCAGCTCC TCGAGCAGGA GCTCGCCCAC GCCGTCAACG CCTCCTCACA TGCCATTAAC
    AAAACACATC CCAAGTTCCC AGAGAGCCTG ACAAGAAACA CTGCCATTGA GGTGGCCAGC
    ACCGTGAAGG CCCTGCACAA TGAGACAGAG TCTCTGTTAG TAGGACGCGT GCCATTGCAA
    CTGGACCCTC CGCATGAAGA TCTGCTGTCC ACTGCGCTGC AGAACGTTGA GGTTGAACTA
    GGCAAGCTGG TTGAGATCCC TTGGCTCTAC CACATCTTAC AGCCCAATGA TGAGGAGGAT
    CCCTCCCTTG AGTATGGAAA AAGAAACAGT CGGAGTGGCA TGTTCCGCAT AGTGCCAAAG
    TTCAAGAAAG AGAAAGTGCC CAAGAAGTCT AGCCCACAAT CAGGATCTGG GGATCTCGAA
    GGTGGGCCAT ATGAAGAGAA TTCTCCAGGG AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009302860.1
    CDS649..4404
    Translation

    Target ORF information:

    RefSeq Version XM_009304585.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio diacylglycerol kinase, eta (dgkh), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009304585.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGACGGCCA AGAGAACCGC TTTCCAGCCG TTGTCCAGCT CCCGCATCCC AGCGGAGAGG 
    AAGGGAGCGG CTGAGCGCGT TCCTAACCCG GAGTGCTGTG ACTTTCCCCC GCAGGAGCCG
    GAGAAAGCAG CTGCGTCCGC CTCGGGGGTT CCCGCCGTGG GGGCTGTCGG GGGCTCGGGG
    CCGGTGGTGG ACGAGTCATC CGACAGCGAA GGAGAGCAGG AAGGGCCGCA GAAACTCATC
    AGGAAAGTGT CAACCTCCGG GCAAATACGA AGCAAGACAA GCGTGAAGGA AGGGCTTCTT
    CTGAAACAGA CAAGCTCGTT CCAGCGTTGG AAAAAACGTT ATTTCAAGCT ACGAGGACGG
    ACGCTCTACT ATGCTAAGGA TGCCAAGGCG GGTGGCGTTT TGGCCCGCTG GCAGTATCGG
    GTCTCTCGTA GCCGCGTGTG CTGGAGGGTG CTTTCCGTGT GCTGGACCGG TGCCCGCCGT
    CCGCCGCCCG CTCTTGGCGC CAGTGGATCG GAGGAAGGCT TGGTGGCGAT TCGGTCTCTT
    ATTTTCGATG AGGTGGACCT TTCAGATGCC AGTGTTGCCG AATCAAGTAC AAAGAATATC
    AACAACAGTT TCACGGTAAT TACACCGTTT CGAAGGCTTA TCCTGTGCGC CGAGAACAGG
    AAAGAAATGG AGGACTGGAT CAGCTCGCTG AAATCGGTTC AGTCCAGAGA GCATTACGAG
    ACGGCACAGT TTAATGTGGA ACATTTCTCA GGGATGCACA ACTGGTATGC CTGCTCCCAC
    GCTCGCCCCA CCTTCTGTAA CGTCTGTCGG GACAGCCTCT CAGGGGTCAC TTCTCATGGC
    CTCTCCTGTG AAGTGTGCAA ATTCAAAGCC CACAAAAGAT GTGCAGTCAG GGCCACTAAC
    AACTGCAAAT GGACAACATT GGCCTCCATC GGGAAGGACA TTATTGAAGA CGAGGATGGG
    ATTGCAATGC CGCACCAATG GCTGGAAGGC AACCTGCCGG TTAGTGCAAA GTGTGCTGTT
    TGCGACAAAA CATGTGGCAG CGTGCTGCGC TTGCAGGACT GGCGCTGTTT GTGGTGCAAG
    GCCATGGTGC ACACGGCATG CATGGATCTC TACCCCCGCA AATGTCCCCT CGGCCAGTGC
    AAAGTGTCCA TCATCCCCCC CACTGCCCTT AACAGCATCG ACTCAGACGG TTTTTGGAAG
    GCCACGTGCC CTCCATCATG TGCAAGTCCG CTCCTCGTGT TTGTCAACTC AAAAAGTGGA
    GACAACCAAG GGGTAAAATT CCTGCGTCGC TTCAAACAGC TGCTCAATCC TGCTCAGGTC
    TTTGACCTGG TCAACGGTGG ACCTCATTTA GGGCTTCGCT TGTTCCAAAA GTTTGACAAC
    TTCCGGATCC TTGTGTGTGG TGGAGATGGA AGCGTTGGCT GGGTGCTGTC TGAAATCGAT
    AAACTAAATC TTCATAAACA GTGCCAGCTG GGTGTTCTGC CGTTGGGCAC CGGGAATGAC
    CTAGCACGGG TGCTAGGTTG GGGGCCGTCA TGTGATGATG ACACTCAGCT GCCTCAGATA
    CTGGAGAAGC TGGAGCGAGC TAGCACCAAA ATGCTGGACA GGTGGAGTAT AATGACGTAT
    GAGATTAAAA TACCTCCCAA ACACAGCTGT CCTGCTACGC CTGAGGAGGC AGAGGACGGC
    CAGTTACAGA TATCAGCTTA TGAGGATTCA GTGGCTGCTC ATCTCACAAA GATTCTGAAC
    TCAGATCAGC ACTCAGTGGT CATATCTTCA TCAAAAGTAT TGTGTGAGAC GGTGAAGGAA
    TTTGTGTCAA GAGTTGGAAA GTGTTACGAG AGAGGCAGCG ACAGCTCAGA AGAATCTGAT
    GCACTGGCTC TCAAATGTGC AATGCTGAAT GAGAAGCTGG AGTCACTCCT GCAGACGTTA
    AATTTGGAGG CTCAGGTCCT GTCGCCCACA GCCCTTACCA CACCACCCAT CGTGGAGGAG
    GAGGTGGAGG AGGAAGAGCT GGAGGAGGAA GACGAAGATC TGAGCGAAGA GTCTCTAACA
    GAGTTGAAGG AGAACGAGAC GGAGAAAGGA TCCGCTCATA AACTCTTCAG ACCCAGAGAG
    CAGCTGGTGC TGAGGGCCAA CAGCCTGAAG AAAGCCCTCA GACAGATCAT CGAGCAGGCT
    GAGAGAATGG TGGATGAACA GAACGCACAC ACAGAGGAGC AGGAGCTTCA GTCTCCTTCT
    GACTTCAGGA AGGACAGCGA AGAGGAAAAC AAAGACAACG AGAAGGACGA GGACACTAAA
    GAACTCGAAA CTCTGCCTTT GGCAAAGTCT CCATGTTCTC CTCCAGAGAG ACGCATCAGT
    CGCAGCACAC AGTCCTGCGG ATCCTTCTCC ATCACTCCCT TCACCACAAG CAAAGAGAAC
    CTGCCCGTTC TCAACACCCG CATCATCTGT CCCGGTCTGC GGGCAGGTCT CGCTGCCTCC
    ATCGCTGGAA GCTCTATAAT CAGTAAAATG CTGCTGGCCA ACATTGATCC GTTTGGTGCC
    ACGCCATTCA TAGATCCTGA CCTAGATTCA CTAGAGGGCT ATTTGGAGAA GTGTGTGATG
    AATAACTACT TTGGGATCGG CTTGGATGCA AAGATCTCAC TGGAGTTCAA CAACAAGCGT
    GAGGAGCATC CGGAGAAGTG CAGGAGTCGC ACTAAAAACA TGATGTGGTA TGGTGTTCTG
    GGGACAAAAG AGTTGCTGCA GAGGACTTAT AAGAACCTGG AACAGAAAGT TCAGCTGGAG
    TGTGATGGCC AGTATATCCC ACTCCCAAGT CTCCAAGGAA TTGCTGTGCT GAACATCCCA
    AGCTATGCTG GGGGAACCAA CTTCTGGGGC GGCACCAAAG AGGATGATAT ATTTTGTGCC
    CCTTCATTCG ATGATAAAAT TTTGGAAGTG GTCGCTGTGT TTGGTAGTAT GCAGATGGCT
    GTCTCAAGGG TGATTAAACT GCAGCATCAC CGTATTGCAC AGTGTCGCAC GGTGAAGATC
    ACAATCCTGG GGGATGAAGG AGTGCCTGTC CAGGTGGATG GTGAAGCCTG GATTCAACCT
    GCTGGAGTCA TCAAGATTCA GCACAAGAAC CGGGCCCAGA TGCTGACAAG AGACCGGGCG
    TTTGAAAACA CGTTGAAGTC TTGGGAGGAT AAATTGAAAT ACGACAAGCC TCCGCTGCGG
    CCACATCTGT ACCCTCAGCA GTCTGTGGAT CTGGCCACAG AGGAGGAGGC CACGCTCATC
    CAGATGTGTG CCCGCGCTGC TGAGGATCTC ATCACCAGGA TCTGTGAGGC TGCCAAAAAC
    TACCAGCTCC TCGAGCAGGA GCTCGCCCAC GCCGTCAACG CCTCCTCACA TGCCATTAAC
    AAAACACATC CCAAGTTCCC AGAGAGCCTG ACAAGAAACA CTGCCATTGA GGTGGCCAGC
    ACCGTGAAGG CCCTGCACAA TGAGACAGAG TCTCTGTTAG TAGGACGCGT GCCATTGCAA
    CTGGACCCTC CGCATGAAGA TCTGCTGTCC ACTGCGCTGC AGAACGTTGA GGTTGAACTA
    GGCAAGCTGG TTGAGATCCC TTGGCTCTAC CACATCTTAC AGCCCAATGA TGAGGAGGAT
    CCCTCCCTTG AGTATGGAAA AAGAAACAGT CGGAGTGGCA TGTTCCGCAT AGTGCCAAAG
    TTCAAGAAAG AGAAAGTGCC CAAGAAGTCT AGCCCACAAT CAGGATCTGG GGATCTCGAA
    GGTGGGCCAT ATGAAGAGAA TTCTCCAGGG AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48524
    Clone ID Related Accession (Same CDS sequence) XM_009304585.2 , XM_009304585.3
    Accession Version XM_009304585.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3756bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product diacylglycerol kinase eta isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304585.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGACGGCCA AGAGAACCGC TTTCCAGCCG TTGTCCAGCT CCCGCATCCC AGCGGAGAGG 
    AAGGGAGCGG CTGAGCGCGT TCCTAACCCG GAGTGCTGTG ACTTTCCCCC GCAGGAGCCG
    GAGAAAGCAG CTGCGTCCGC CTCGGGGGTT CCCGCCGTGG GGGCTGTCGG GGGCTCGGGG
    CCGGTGGTGG ACGAGTCATC CGACAGCGAA GGAGAGCAGG AAGGGCCGCA GAAACTCATC
    AGGAAAGTGT CAACCTCCGG GCAAATACGA AGCAAGACAA GCGTGAAGGA AGGGCTTCTT
    CTGAAACAGA CAAGCTCGTT CCAGCGTTGG AAAAAACGTT ATTTCAAGCT ACGAGGACGG
    ACGCTCTACT ATGCTAAGGA TGCCAAGGCG GGTGGCGTTT TGGCCCGCTG GCAGTATCGG
    GTCTCTCGTA GCCGCGTGTG CTGGAGGGTG CTTTCCGTGT GCTGGACCGG TGCCCGCCGT
    CCGCCGCCCG CTCTTGGCGC CAGTGGATCG GAGGAAGGCT TGGTGGCGAT TCGGTCTCTT
    ATTTTCGATG AGGTGGACCT TTCAGATGCC AGTGTTGCCG AATCAAGTAC AAAGAATATC
    AACAACAGTT TCACGGTAAT TACACCGTTT CGAAGGCTTA TCCTGTGCGC CGAGAACAGG
    AAAGAAATGG AGGACTGGAT CAGCTCGCTG AAATCGGTTC AGTCCAGAGA GCATTACGAG
    ACGGCACAGT TTAATGTGGA ACATTTCTCA GGGATGCACA ACTGGTATGC CTGCTCCCAC
    GCTCGCCCCA CCTTCTGTAA CGTCTGTCGG GACAGCCTCT CAGGGGTCAC TTCTCATGGC
    CTCTCCTGTG AAGTGTGCAA ATTCAAAGCC CACAAAAGAT GTGCAGTCAG GGCCACTAAC
    AACTGCAAAT GGACAACATT GGCCTCCATC GGGAAGGACA TTATTGAAGA CGAGGATGGG
    ATTGCAATGC CGCACCAATG GCTGGAAGGC AACCTGCCGG TTAGTGCAAA GTGTGCTGTT
    TGCGACAAAA CATGTGGCAG CGTGCTGCGC TTGCAGGACT GGCGCTGTTT GTGGTGCAAG
    GCCATGGTGC ACACGGCATG CATGGATCTC TACCCCCGCA AATGTCCCCT CGGCCAGTGC
    AAAGTGTCCA TCATCCCCCC CACTGCCCTT AACAGCATCG ACTCAGACGG TTTTTGGAAG
    GCCACGTGCC CTCCATCATG TGCAAGTCCG CTCCTCGTGT TTGTCAACTC AAAAAGTGGA
    GACAACCAAG GGGTAAAATT CCTGCGTCGC TTCAAACAGC TGCTCAATCC TGCTCAGGTC
    TTTGACCTGG TCAACGGTGG ACCTCATTTA GGGCTTCGCT TGTTCCAAAA GTTTGACAAC
    TTCCGGATCC TTGTGTGTGG TGGAGATGGA AGCGTTGGCT GGGTGCTGTC TGAAATCGAT
    AAACTAAATC TTCATAAACA GTGCCAGCTG GGTGTTCTGC CGTTGGGCAC CGGGAATGAC
    CTAGCACGGG TGCTAGGTTG GGGGCCGTCA TGTGATGATG ACACTCAGCT GCCTCAGATA
    CTGGAGAAGC TGGAGCGAGC TAGCACCAAA ATGCTGGACA GGTGGAGTAT AATGACGTAT
    GAGATTAAAA TACCTCCCAA ACACAGCTGT CCTGCTACGC CTGAGGAGGC AGAGGACGGC
    CAGTTACAGA TATCAGCTTA TGAGGATTCA GTGGCTGCTC ATCTCACAAA GATTCTGAAC
    TCAGATCAGC ACTCAGTGGT CATATCTTCA TCAAAAGTAT TGTGTGAGAC GGTGAAGGAA
    TTTGTGTCAA GAGTTGGAAA GTGTTACGAG AGAGGCAGCG ACAGCTCAGA AGAATCTGAT
    GCACTGGCTC TCAAATGTGC AATGCTGAAT GAGAAGCTGG AGTCACTCCT GCAGACGTTA
    AATTTGGAGG CTCAGGTCCT GTCGCCCACA GCCCTTACCA CACCACCCAT CGTGGAGGAG
    GAGGTGGAGG AGGAAGAGCT GGAGGAGGAA GACGAAGATC TGAGCGAAGA GTCTCTAACA
    GAGTTGAAGG AGAACGAGAC GGAGAAAGGA TCCGCTCATA AACTCTTCAG ACCCAGAGAG
    CAGCTGGTGC TGAGGGCCAA CAGCCTGAAG AAAGCCCTCA GACAGATCAT CGAGCAGGCT
    GAGAGAATGG TGGATGAACA GAACGCACAC ACAGAGGAGC AGGAGCTTCA GTCTCCTTCT
    GACTTCAGGA AGGACAGCGA AGAGGAAAAC AAAGACAACG AGAAGGACGA GGACACTAAA
    GAACTCGAAA CTCTGCCTTT GGCAAAGTCT CCATGTTCTC CTCCAGAGAG ACGCATCAGT
    CGCAGCACAC AGTCCTGCGG ATCCTTCTCC ATCACTCCCT TCACCACAAG CAAAGAGAAC
    CTGCCCGTTC TCAACACCCG CATCATCTGT CCCGGTCTGC GGGCAGGTCT CGCTGCCTCC
    ATCGCTGGAA GCTCTATAAT CAGTAAAATG CTGCTGGCCA ACATTGATCC GTTTGGTGCC
    ACGCCATTCA TAGATCCTGA CCTAGATTCA CTAGAGGGCT ATTTGGAGAA GTGTGTGATG
    AATAACTACT TTGGGATCGG CTTGGATGCA AAGATCTCAC TGGAGTTCAA CAACAAGCGT
    GAGGAGCATC CGGAGAAGTG CAGGAGTCGC ACTAAAAACA TGATGTGGTA TGGTGTTCTG
    GGGACAAAAG AGTTGCTGCA GAGGACTTAT AAGAACCTGG AACAGAAAGT TCAGCTGGAG
    TGTGATGGCC AGTATATCCC ACTCCCAAGT CTCCAAGGAA TTGCTGTGCT GAACATCCCA
    AGCTATGCTG GGGGAACCAA CTTCTGGGGC GGCACCAAAG AGGATGATAT ATTTTGTGCC
    CCTTCATTCG ATGATAAAAT TTTGGAAGTG GTCGCTGTGT TTGGTAGTAT GCAGATGGCT
    GTCTCAAGGG TGATTAAACT GCAGCATCAC CGTATTGCAC AGTGTCGCAC GGTGAAGATC
    ACAATCCTGG GGGATGAAGG AGTGCCTGTC CAGGTGGATG GTGAAGCCTG GATTCAACCT
    GCTGGAGTCA TCAAGATTCA GCACAAGAAC CGGGCCCAGA TGCTGACAAG AGACCGGGCG
    TTTGAAAACA CGTTGAAGTC TTGGGAGGAT AAATTGAAAT ACGACAAGCC TCCGCTGCGG
    CCACATCTGT ACCCTCAGCA GTCTGTGGAT CTGGCCACAG AGGAGGAGGC CACGCTCATC
    CAGATGTGTG CCCGCGCTGC TGAGGATCTC ATCACCAGGA TCTGTGAGGC TGCCAAAAAC
    TACCAGCTCC TCGAGCAGGA GCTCGCCCAC GCCGTCAACG CCTCCTCACA TGCCATTAAC
    AAAACACATC CCAAGTTCCC AGAGAGCCTG ACAAGAAACA CTGCCATTGA GGTGGCCAGC
    ACCGTGAAGG CCCTGCACAA TGAGACAGAG TCTCTGTTAG TAGGACGCGT GCCATTGCAA
    CTGGACCCTC CGCATGAAGA TCTGCTGTCC ACTGCGCTGC AGAACGTTGA GGTTGAACTA
    GGCAAGCTGG TTGAGATCCC TTGGCTCTAC CACATCTTAC AGCCCAATGA TGAGGAGGAT
    CCCTCCCTTG AGTATGGAAA AAGAAACAGT CGGAGTGGCA TGTTCCGCAT AGTGCCAAAG
    TTCAAGAAAG AGAAAGTGCC CAAGAAGTCT AGCCCACAAT CAGGATCTGG GGATCTCGAA
    GGTGGGCCAT ATGAAGAGAA TTCTCCAGGG AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009302860.1
    CDS803..4558
    Translation

    Target ORF information:

    RefSeq Version XM_009304585.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio diacylglycerol kinase, eta (dgkh), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009304585.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGACGGCCA AGAGAACCGC TTTCCAGCCG TTGTCCAGCT CCCGCATCCC AGCGGAGAGG 
    AAGGGAGCGG CTGAGCGCGT TCCTAACCCG GAGTGCTGTG ACTTTCCCCC GCAGGAGCCG
    GAGAAAGCAG CTGCGTCCGC CTCGGGGGTT CCCGCCGTGG GGGCTGTCGG GGGCTCGGGG
    CCGGTGGTGG ACGAGTCATC CGACAGCGAA GGAGAGCAGG AAGGGCCGCA GAAACTCATC
    AGGAAAGTGT CAACCTCCGG GCAAATACGA AGCAAGACAA GCGTGAAGGA AGGGCTTCTT
    CTGAAACAGA CAAGCTCGTT CCAGCGTTGG AAAAAACGTT ATTTCAAGCT ACGAGGACGG
    ACGCTCTACT ATGCTAAGGA TGCCAAGGCG GGTGGCGTTT TGGCCCGCTG GCAGTATCGG
    GTCTCTCGTA GCCGCGTGTG CTGGAGGGTG CTTTCCGTGT GCTGGACCGG TGCCCGCCGT
    CCGCCGCCCG CTCTTGGCGC CAGTGGATCG GAGGAAGGCT TGGTGGCGAT TCGGTCTCTT
    ATTTTCGATG AGGTGGACCT TTCAGATGCC AGTGTTGCCG AATCAAGTAC AAAGAATATC
    AACAACAGTT TCACGGTAAT TACACCGTTT CGAAGGCTTA TCCTGTGCGC CGAGAACAGG
    AAAGAAATGG AGGACTGGAT CAGCTCGCTG AAATCGGTTC AGTCCAGAGA GCATTACGAG
    ACGGCACAGT TTAATGTGGA ACATTTCTCA GGGATGCACA ACTGGTATGC CTGCTCCCAC
    GCTCGCCCCA CCTTCTGTAA CGTCTGTCGG GACAGCCTCT CAGGGGTCAC TTCTCATGGC
    CTCTCCTGTG AAGTGTGCAA ATTCAAAGCC CACAAAAGAT GTGCAGTCAG GGCCACTAAC
    AACTGCAAAT GGACAACATT GGCCTCCATC GGGAAGGACA TTATTGAAGA CGAGGATGGG
    ATTGCAATGC CGCACCAATG GCTGGAAGGC AACCTGCCGG TTAGTGCAAA GTGTGCTGTT
    TGCGACAAAA CATGTGGCAG CGTGCTGCGC TTGCAGGACT GGCGCTGTTT GTGGTGCAAG
    GCCATGGTGC ACACGGCATG CATGGATCTC TACCCCCGCA AATGTCCCCT CGGCCAGTGC
    AAAGTGTCCA TCATCCCCCC CACTGCCCTT AACAGCATCG ACTCAGACGG TTTTTGGAAG
    GCCACGTGCC CTCCATCATG TGCAAGTCCG CTCCTCGTGT TTGTCAACTC AAAAAGTGGA
    GACAACCAAG GGGTAAAATT CCTGCGTCGC TTCAAACAGC TGCTCAATCC TGCTCAGGTC
    TTTGACCTGG TCAACGGTGG ACCTCATTTA GGGCTTCGCT TGTTCCAAAA GTTTGACAAC
    TTCCGGATCC TTGTGTGTGG TGGAGATGGA AGCGTTGGCT GGGTGCTGTC TGAAATCGAT
    AAACTAAATC TTCATAAACA GTGCCAGCTG GGTGTTCTGC CGTTGGGCAC CGGGAATGAC
    CTAGCACGGG TGCTAGGTTG GGGGCCGTCA TGTGATGATG ACACTCAGCT GCCTCAGATA
    CTGGAGAAGC TGGAGCGAGC TAGCACCAAA ATGCTGGACA GGTGGAGTAT AATGACGTAT
    GAGATTAAAA TACCTCCCAA ACACAGCTGT CCTGCTACGC CTGAGGAGGC AGAGGACGGC
    CAGTTACAGA TATCAGCTTA TGAGGATTCA GTGGCTGCTC ATCTCACAAA GATTCTGAAC
    TCAGATCAGC ACTCAGTGGT CATATCTTCA TCAAAAGTAT TGTGTGAGAC GGTGAAGGAA
    TTTGTGTCAA GAGTTGGAAA GTGTTACGAG AGAGGCAGCG ACAGCTCAGA AGAATCTGAT
    GCACTGGCTC TCAAATGTGC AATGCTGAAT GAGAAGCTGG AGTCACTCCT GCAGACGTTA
    AATTTGGAGG CTCAGGTCCT GTCGCCCACA GCCCTTACCA CACCACCCAT CGTGGAGGAG
    GAGGTGGAGG AGGAAGAGCT GGAGGAGGAA GACGAAGATC TGAGCGAAGA GTCTCTAACA
    GAGTTGAAGG AGAACGAGAC GGAGAAAGGA TCCGCTCATA AACTCTTCAG ACCCAGAGAG
    CAGCTGGTGC TGAGGGCCAA CAGCCTGAAG AAAGCCCTCA GACAGATCAT CGAGCAGGCT
    GAGAGAATGG TGGATGAACA GAACGCACAC ACAGAGGAGC AGGAGCTTCA GTCTCCTTCT
    GACTTCAGGA AGGACAGCGA AGAGGAAAAC AAAGACAACG AGAAGGACGA GGACACTAAA
    GAACTCGAAA CTCTGCCTTT GGCAAAGTCT CCATGTTCTC CTCCAGAGAG ACGCATCAGT
    CGCAGCACAC AGTCCTGCGG ATCCTTCTCC ATCACTCCCT TCACCACAAG CAAAGAGAAC
    CTGCCCGTTC TCAACACCCG CATCATCTGT CCCGGTCTGC GGGCAGGTCT CGCTGCCTCC
    ATCGCTGGAA GCTCTATAAT CAGTAAAATG CTGCTGGCCA ACATTGATCC GTTTGGTGCC
    ACGCCATTCA TAGATCCTGA CCTAGATTCA CTAGAGGGCT ATTTGGAGAA GTGTGTGATG
    AATAACTACT TTGGGATCGG CTTGGATGCA AAGATCTCAC TGGAGTTCAA CAACAAGCGT
    GAGGAGCATC CGGAGAAGTG CAGGAGTCGC ACTAAAAACA TGATGTGGTA TGGTGTTCTG
    GGGACAAAAG AGTTGCTGCA GAGGACTTAT AAGAACCTGG AACAGAAAGT TCAGCTGGAG
    TGTGATGGCC AGTATATCCC ACTCCCAAGT CTCCAAGGAA TTGCTGTGCT GAACATCCCA
    AGCTATGCTG GGGGAACCAA CTTCTGGGGC GGCACCAAAG AGGATGATAT ATTTTGTGCC
    CCTTCATTCG ATGATAAAAT TTTGGAAGTG GTCGCTGTGT TTGGTAGTAT GCAGATGGCT
    GTCTCAAGGG TGATTAAACT GCAGCATCAC CGTATTGCAC AGTGTCGCAC GGTGAAGATC
    ACAATCCTGG GGGATGAAGG AGTGCCTGTC CAGGTGGATG GTGAAGCCTG GATTCAACCT
    GCTGGAGTCA TCAAGATTCA GCACAAGAAC CGGGCCCAGA TGCTGACAAG AGACCGGGCG
    TTTGAAAACA CGTTGAAGTC TTGGGAGGAT AAATTGAAAT ACGACAAGCC TCCGCTGCGG
    CCACATCTGT ACCCTCAGCA GTCTGTGGAT CTGGCCACAG AGGAGGAGGC CACGCTCATC
    CAGATGTGTG CCCGCGCTGC TGAGGATCTC ATCACCAGGA TCTGTGAGGC TGCCAAAAAC
    TACCAGCTCC TCGAGCAGGA GCTCGCCCAC GCCGTCAACG CCTCCTCACA TGCCATTAAC
    AAAACACATC CCAAGTTCCC AGAGAGCCTG ACAAGAAACA CTGCCATTGA GGTGGCCAGC
    ACCGTGAAGG CCCTGCACAA TGAGACAGAG TCTCTGTTAG TAGGACGCGT GCCATTGCAA
    CTGGACCCTC CGCATGAAGA TCTGCTGTCC ACTGCGCTGC AGAACGTTGA GGTTGAACTA
    GGCAAGCTGG TTGAGATCCC TTGGCTCTAC CACATCTTAC AGCCCAATGA TGAGGAGGAT
    CCCTCCCTTG AGTATGGAAA AAGAAACAGT CGGAGTGGCA TGTTCCGCAT AGTGCCAAAG
    TTCAAGAAAG AGAAAGTGCC CAAGAAGTCT AGCCCACAAT CAGGATCTGG GGATCTCGAA
    GGTGGGCCAT ATGAAGAGAA TTCTCCAGGG AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144060
    Clone ID Related Accession (Same CDS sequence) XM_021478876.1
    Accession Version XM_021478876.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3990bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product diacylglycerol kinase eta isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGACGGCCA AGAGAACCGC TTTCCAGCCG TTGTCCAGCT CCCGCATCCC AGCGGAGAGG 
    AAGGGAGCGG CTGAGCGCGT TCCTAACCCG GAGTGCTGTG ACTTTCCCCC GCAGGAGCCG
    GAGAAAGCAG CTGCGTCCGC CTCGGGGGTT CCCGCCGTGG GGGCTGTCGG GGGCTCGGGG
    CCGGTGGTGG ACGAGTCATC CGACAGCGAA GGAGAGCAGG AAGGGCCGCA GAAACTCATC
    AGGAAAGTGT CAACCTCCGG GCAAATACGA AGCAAGACAA GCGTGAAGGA AGGGCTTCTT
    CTGAAACAGA CAAGCTCGTT CCAGCGTTGG AAAAAACGTT ATTTCAAGCT ACGAGGACGG
    ACGCTCTACT ATGCTAAGGA TGCCAAGGCG GGTGGCGTTT TGGCCCGCTG GCAGTATCGG
    GTCTCTCGTA GCCGCGTGTG CTGGAGGGTG CTTTCCGTGT GCTGGACCGG TGCCCGCCGT
    CCGCCGCCCG CTCTTGGCGC CAGTGGATCG GAGGAAGGCT TGGTGGCGAT TCGGTCTCTT
    ATTTTCGATG AGGTGGACCT TTCAGATGCC AGTGTTGCCG AATCAAGTAC AAAGAATATC
    AACAACAGTT TCACGGTAAT TACACCGTTT CGAAGGCTTA TCCTGTGCGC CGAGAACAGG
    AAAGAAATGG AGGACTGGAT CAGCTCGCTG AAATCGGTTC AGTCCAGAGA GCATTACGAG
    ACGGCACAGT TTAATGTGGA ACATTTCTCA GGGATGCACA ACTGGTATGC CTGCTCCCAC
    GCTCGCCCCA CCTTCTGTAA CGTCTGTCGG GACAGCCTCT CAGGGGTCAC TTCTCATGGC
    CTCTCCTGTG AAGTGTGCAA ATTCAAAGCC CACAAAAGAT GTGCAGTCAG GGCCACTAAC
    AACTGCAAAT GGACAACATT GGCCTCCATC GGGAAGGACA TTATTGAAGA CGAGGATGGG
    ATTGCAATGC CGCACCAATG GCTGGAAGGC AACCTGCCGG TTAGTGCAAA GTGTGCTGTT
    TGCGACAAAA CATGTGGCAG CGTGCTGCGC TTGCAGGACT GGCGCTGTTT GTGGTGCAAG
    GCCATGGTGC ACACGGCATG CATGGATCTC TACCCCCGCA AATGTCCCCT CGGCCAGTGC
    AAAGTGTCCA TCATCCCCCC CACTGCCCTT AACAGCATCG ACTCAGACGG TTTTTGGAAG
    GCCACGTGCC CTCCATCATG TGCAAGTCCG CTCCTCGTGT TTGTCAACTC AAAAAGTGGA
    GACAACCAAG GGGTAAAATT CCTGCGTCGC TTCAAACAGC TGCTCAATCC TGCTCAGGTC
    TTTGACCTGG TCAACGGTGG ACCTCATTTA GGGCTTCGCT TGTTCCAAAA GTTTGACAAC
    TTCCGGATCC TTGTGTGTGG TGGAGATGGA AGCGTTGGCT GGGTGCTGTC TGAAATCGAT
    AAACTAAATC TTCATAAACA GTGCCAGCTG GGTGTTCTGC CGTTGGGCAC CGGGAATGAC
    CTAGCACGGG TGCTAGGTTG GGGGCCGTCA TGTGATGATG ACACTCAGCT GCCTCAGATA
    CTGGAGAAGC TGGAGCGAGC TAGCACCAAA ATGCTGGACA GGTGGAGTAT AATGACGTAT
    GAGATTAAAA TACCTCCCAA ACACAGCTGT CCTGCTACGC CTGAGGAGGC AGAGGACGGC
    CAGTTACAGA TATCAGCTTA TGAGGATTCA GTGGCTGCTC ATCTCACAAA GATTCTGAAC
    TCAGATCAGC ACTCAGTGGT CATATCTTCA TCAAAAGTAT TGTGTGAGAC GGTGAAGGAA
    TTTGTGTCAA GAGTTGGAAA GTGTTACGAG AGAGGCAGCG ACAGCTCAGA AGAATCTGAT
    GCACTGGCTC TCAAATGTGC AATGCTGAAT GAGAAGCTGG AGTCACTCCT GCAGACGTTA
    AATTTGGAGG CTCAGGTCCT GTCGCCCACA GCCCTTACCA CACCACCCAT CGTGGAGGAG
    GAGGTGGAGG AGGAAGAGCT GGAGGAGGAA GACGAAGATC TGAGCGAAGA GTCTCTAACA
    GAGTTGAAGG AGAACGAGAC GGAGAAAGGA TCCGCTCATA AACTCTTCAG ACCCAGAGAG
    CAGCTGGTGC TGAGGGCCAA CAGCCTGAAG AAAGCCCTCA GACAGATCAT CGAGCAGGCT
    GAGAGAATGG TGGATGAACA GAACGCACAC ACAGAGGAGC AGGAGCTTCA GTCTCCTTCT
    GACTTCAGGA AGGACAGCGA AGAGGAAAAC AAAGACAACG AGAAGGACGA GGACACTAAA
    GAACTCGAAA CTCTGCCTTT GGCAAAGTCT CCATGTTCTC CTCCAGAGAG ACGCATCAGT
    CGCAGCACAC AGTCCTGCGG ATCCTTCTCC ATCACTCCCT TCACCACAAG CAAAGAGAAC
    CTGCCCGTTC TCAACACCCG CATCATCTGT CCCGGTCTGC GGGCAGGTCT CGCTGCCTCC
    ATCGCTGGAA GCTCTATAAT CAGTAAAATG CTGCTGGCCA ACATTGATCC GTTTGGTGCC
    ACGCCATTCA TAGATCCTGA CCTAGATTCA CTAGAGGGCT ATTTGGAGAA GTGTGTGATG
    AATAACTACT TTGGGATCGG CTTGGATGCA AAGATCTCAC TGGAGTTCAA CAACAAGCGT
    GAGGAGCATC CGGAGAAGTG CAGGAGTCGC ACTAAAAACA TGATGTGGTA TGGTGTTCTG
    GGGACAAAAG AGTTGCTGCA GAGGACTTAT AAGAACCTGG AACAGAAAGT TCAGCTGGAG
    TGTGATGGCC AGTATATCCC ACTCCCAAGT CTCCAAGGAA TTGCTGTGCT GAACATCCCA
    AGCTATGCTG GGGGAACCAA CTTCTGGGGC GGCACCAAAG AGGATGATAT ATTTTGTGCC
    CCTTCATTCG ATGATAAAAT TTTGGAAGTG GTCGCTGTGT TTGGTAGTAT GCAGATGGCT
    GTCTCAAGGG TGATTAAACT GCAGCATCAC CGTATTGCAC AGTGTCGCAC GGTGAAGATC
    ACAATCCTGG GGGATGAAGG AGTGCCTGTC CAGGTGGATG GTGAAGCCTG GATTCAACCT
    GCTGGAGTCA TCAAGATTCA GCACAAGAAC CGGGCCCAGA TGCTGACAAG AGACCGGGCG
    TTTGAAAACA CGTTGAAGTC TTGGGAGGAT AAATTGAAAT ACGACAAGCC TCCGCTGCGG
    CCACATCTGT ACCCTCAGCA GTCTGTGGAT CTGGCCACAG AGGAGGAGGC CACGCTCATC
    CAGATGTGTG CCCGCGCTGC TGAGGATCTC ATCACCAGGA TCTGTGAGGC TGCCAAAAAC
    TACCAGCTCC TCGAGCAGGA GCTCGCCCAC GCCGTCAACG CCTCCTCACA TGCCATTAAC
    AAAACACATC CCAAGTTCCC AGAGAGCCTG ACAAGAAACA CTGCCATTGA GGTGGCCAGC
    ACCGTGAAGG CCCTGCACAA TGAGACAGAG TCTCTGTTAG TAGGACGCGT GCCATTGCAA
    CTGGACCCTC CGCATGAAGA TCTGCTGTCC ACTGCGCTGC AGAACGTTGA GGTTGAACTA
    GGCAAGCTGG TTGAGATCCC TTGGCTCTAC CACATCTTAC AGCCCAATGA TGAGGAGGAT
    CCCTCCCTTG AGTATGGAAA AAGAAACAGT CGGAGTGGCA TGTTCCGCAT AGTGCCAAAG
    TTCAAGAAAG AGAAAGTGCC CAAGAAGTCT AGCCCACAAT CAGACGATGT CCATGGACAT
    ACGGAGGCAG TGACACGCAG CCTGTGTGAT GAAATAGAGC GCCATTCAGC ATTTCAGAGG
    TGGGGTACTG AGGAGGTGGC CATCTGGCTG GAGCAGCTCA GCTTGGGAGA GTACAAAGAC
    ACCTTCATCC GCCACGACAT CCGCGGCTCC GAGCTCCTGC ACCTGGAGAG GAGAGACCTT
    AAGGATCTGG GGATCTCGAA GGTGGGCCAT ATGAAGAGAA TTCTCCAGGG AACTAAGGAC
    TTGGCCAAGA GTTCGATGGT GGACCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021334551.1
    CDS803..4792
    Translation

    Target ORF information:

    RefSeq Version XM_021478876.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio diacylglycerol kinase, eta (dgkh), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021478876.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGACGGCCA AGAGAACCGC TTTCCAGCCG TTGTCCAGCT CCCGCATCCC AGCGGAGAGG 
    AAGGGAGCGG CTGAGCGCGT TCCTAACCCG GAGTGCTGTG ACTTTCCCCC GCAGGAGCCG
    GAGAAAGCAG CTGCGTCCGC CTCGGGGGTT CCCGCCGTGG GGGCTGTCGG GGGCTCGGGG
    CCGGTGGTGG ACGAGTCATC CGACAGCGAA GGAGAGCAGG AAGGGCCGCA GAAACTCATC
    AGGAAAGTGT CAACCTCCGG GCAAATACGA AGCAAGACAA GCGTGAAGGA AGGGCTTCTT
    CTGAAACAGA CAAGCTCGTT CCAGCGTTGG AAAAAACGTT ATTTCAAGCT ACGAGGACGG
    ACGCTCTACT ATGCTAAGGA TGCCAAGGCG GGTGGCGTTT TGGCCCGCTG GCAGTATCGG
    GTCTCTCGTA GCCGCGTGTG CTGGAGGGTG CTTTCCGTGT GCTGGACCGG TGCCCGCCGT
    CCGCCGCCCG CTCTTGGCGC CAGTGGATCG GAGGAAGGCT TGGTGGCGAT TCGGTCTCTT
    ATTTTCGATG AGGTGGACCT TTCAGATGCC AGTGTTGCCG AATCAAGTAC AAAGAATATC
    AACAACAGTT TCACGGTAAT TACACCGTTT CGAAGGCTTA TCCTGTGCGC CGAGAACAGG
    AAAGAAATGG AGGACTGGAT CAGCTCGCTG AAATCGGTTC AGTCCAGAGA GCATTACGAG
    ACGGCACAGT TTAATGTGGA ACATTTCTCA GGGATGCACA ACTGGTATGC CTGCTCCCAC
    GCTCGCCCCA CCTTCTGTAA CGTCTGTCGG GACAGCCTCT CAGGGGTCAC TTCTCATGGC
    CTCTCCTGTG AAGTGTGCAA ATTCAAAGCC CACAAAAGAT GTGCAGTCAG GGCCACTAAC
    AACTGCAAAT GGACAACATT GGCCTCCATC GGGAAGGACA TTATTGAAGA CGAGGATGGG
    ATTGCAATGC CGCACCAATG GCTGGAAGGC AACCTGCCGG TTAGTGCAAA GTGTGCTGTT
    TGCGACAAAA CATGTGGCAG CGTGCTGCGC TTGCAGGACT GGCGCTGTTT GTGGTGCAAG
    GCCATGGTGC ACACGGCATG CATGGATCTC TACCCCCGCA AATGTCCCCT CGGCCAGTGC
    AAAGTGTCCA TCATCCCCCC CACTGCCCTT AACAGCATCG ACTCAGACGG TTTTTGGAAG
    GCCACGTGCC CTCCATCATG TGCAAGTCCG CTCCTCGTGT TTGTCAACTC AAAAAGTGGA
    GACAACCAAG GGGTAAAATT CCTGCGTCGC TTCAAACAGC TGCTCAATCC TGCTCAGGTC
    TTTGACCTGG TCAACGGTGG ACCTCATTTA GGGCTTCGCT TGTTCCAAAA GTTTGACAAC
    TTCCGGATCC TTGTGTGTGG TGGAGATGGA AGCGTTGGCT GGGTGCTGTC TGAAATCGAT
    AAACTAAATC TTCATAAACA GTGCCAGCTG GGTGTTCTGC CGTTGGGCAC CGGGAATGAC
    CTAGCACGGG TGCTAGGTTG GGGGCCGTCA TGTGATGATG ACACTCAGCT GCCTCAGATA
    CTGGAGAAGC TGGAGCGAGC TAGCACCAAA ATGCTGGACA GGTGGAGTAT AATGACGTAT
    GAGATTAAAA TACCTCCCAA ACACAGCTGT CCTGCTACGC CTGAGGAGGC AGAGGACGGC
    CAGTTACAGA TATCAGCTTA TGAGGATTCA GTGGCTGCTC ATCTCACAAA GATTCTGAAC
    TCAGATCAGC ACTCAGTGGT CATATCTTCA TCAAAAGTAT TGTGTGAGAC GGTGAAGGAA
    TTTGTGTCAA GAGTTGGAAA GTGTTACGAG AGAGGCAGCG ACAGCTCAGA AGAATCTGAT
    GCACTGGCTC TCAAATGTGC AATGCTGAAT GAGAAGCTGG AGTCACTCCT GCAGACGTTA
    AATTTGGAGG CTCAGGTCCT GTCGCCCACA GCCCTTACCA CACCACCCAT CGTGGAGGAG
    GAGGTGGAGG AGGAAGAGCT GGAGGAGGAA GACGAAGATC TGAGCGAAGA GTCTCTAACA
    GAGTTGAAGG AGAACGAGAC GGAGAAAGGA TCCGCTCATA AACTCTTCAG ACCCAGAGAG
    CAGCTGGTGC TGAGGGCCAA CAGCCTGAAG AAAGCCCTCA GACAGATCAT CGAGCAGGCT
    GAGAGAATGG TGGATGAACA GAACGCACAC ACAGAGGAGC AGGAGCTTCA GTCTCCTTCT
    GACTTCAGGA AGGACAGCGA AGAGGAAAAC AAAGACAACG AGAAGGACGA GGACACTAAA
    GAACTCGAAA CTCTGCCTTT GGCAAAGTCT CCATGTTCTC CTCCAGAGAG ACGCATCAGT
    CGCAGCACAC AGTCCTGCGG ATCCTTCTCC ATCACTCCCT TCACCACAAG CAAAGAGAAC
    CTGCCCGTTC TCAACACCCG CATCATCTGT CCCGGTCTGC GGGCAGGTCT CGCTGCCTCC
    ATCGCTGGAA GCTCTATAAT CAGTAAAATG CTGCTGGCCA ACATTGATCC GTTTGGTGCC
    ACGCCATTCA TAGATCCTGA CCTAGATTCA CTAGAGGGCT ATTTGGAGAA GTGTGTGATG
    AATAACTACT TTGGGATCGG CTTGGATGCA AAGATCTCAC TGGAGTTCAA CAACAAGCGT
    GAGGAGCATC CGGAGAAGTG CAGGAGTCGC ACTAAAAACA TGATGTGGTA TGGTGTTCTG
    GGGACAAAAG AGTTGCTGCA GAGGACTTAT AAGAACCTGG AACAGAAAGT TCAGCTGGAG
    TGTGATGGCC AGTATATCCC ACTCCCAAGT CTCCAAGGAA TTGCTGTGCT GAACATCCCA
    AGCTATGCTG GGGGAACCAA CTTCTGGGGC GGCACCAAAG AGGATGATAT ATTTTGTGCC
    CCTTCATTCG ATGATAAAAT TTTGGAAGTG GTCGCTGTGT TTGGTAGTAT GCAGATGGCT
    GTCTCAAGGG TGATTAAACT GCAGCATCAC CGTATTGCAC AGTGTCGCAC GGTGAAGATC
    ACAATCCTGG GGGATGAAGG AGTGCCTGTC CAGGTGGATG GTGAAGCCTG GATTCAACCT
    GCTGGAGTCA TCAAGATTCA GCACAAGAAC CGGGCCCAGA TGCTGACAAG AGACCGGGCG
    TTTGAAAACA CGTTGAAGTC TTGGGAGGAT AAATTGAAAT ACGACAAGCC TCCGCTGCGG
    CCACATCTGT ACCCTCAGCA GTCTGTGGAT CTGGCCACAG AGGAGGAGGC CACGCTCATC
    CAGATGTGTG CCCGCGCTGC TGAGGATCTC ATCACCAGGA TCTGTGAGGC TGCCAAAAAC
    TACCAGCTCC TCGAGCAGGA GCTCGCCCAC GCCGTCAACG CCTCCTCACA TGCCATTAAC
    AAAACACATC CCAAGTTCCC AGAGAGCCTG ACAAGAAACA CTGCCATTGA GGTGGCCAGC
    ACCGTGAAGG CCCTGCACAA TGAGACAGAG TCTCTGTTAG TAGGACGCGT GCCATTGCAA
    CTGGACCCTC CGCATGAAGA TCTGCTGTCC ACTGCGCTGC AGAACGTTGA GGTTGAACTA
    GGCAAGCTGG TTGAGATCCC TTGGCTCTAC CACATCTTAC AGCCCAATGA TGAGGAGGAT
    CCCTCCCTTG AGTATGGAAA AAGAAACAGT CGGAGTGGCA TGTTCCGCAT AGTGCCAAAG
    TTCAAGAAAG AGAAAGTGCC CAAGAAGTCT AGCCCACAAT CAGACGATGT CCATGGACAT
    ACGGAGGCAG TGACACGCAG CCTGTGTGAT GAAATAGAGC GCCATTCAGC ATTTCAGAGG
    TGGGGTACTG AGGAGGTGGC CATCTGGCTG GAGCAGCTCA GCTTGGGAGA GTACAAAGAC
    ACCTTCATCC GCCACGACAT CCGCGGCTCC GAGCTCCTGC ACCTGGAGAG GAGAGACCTT
    AAGGATCTGG GGATCTCGAA GGTGGGCCAT ATGAAGAGAA TTCTCCAGGG AACTAAGGAC
    TTGGCCAAGA GTTCGATGGT GGACCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144061
    Clone ID Related Accession (Same CDS sequence) XM_021478877.1
    Accession Version XM_021478877.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3324bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product diacylglycerol kinase eta isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAGGACT GGATCAGCTC GCTGAAATCG GTTCAGTCCA GAGAGCATTA CGAGACGGCA 
    CAGTTTAATG TGGAACATTT CTCAGGGATG CACAACTGGT ATGCCTGCTC CCACGCTCGC
    CCCACCTTCT GTAACGTCTG TCGGGACAGC CTCTCAGGGG TCACTTCTCA TGGCCTCTCC
    TGTGAAGTGT GCAAATTCAA AGCCCACAAA AGATGTGCAG TCAGGGCCAC TAACAACTGC
    AAATGGACAA CATTGGCCTC CATCGGGAAG GACATTATTG AAGACGAGGA TGGGATTGCA
    ATGCCGCACC AATGGCTGGA AGGCAACCTG CCGGTTAGTG CAAAGTGTGC TGTTTGCGAC
    AAAACATGTG GCAGCGTGCT GCGCTTGCAG GACTGGCGCT GTTTGTGGTG CAAGGCCATG
    GTGCACACGG CATGCATGGA TCTCTACCCC CGCAAATGTC CCCTCGGCCA GTGCAAAGTG
    TCCATCATCC CCCCCACTGC CCTTAACAGC ATCGACTCAG ACGGTTTTTG GAAGGCCACG
    TGCCCTCCAT CATGTGCAAG TCCGCTCCTC GTGTTTGTCA ACTCAAAAAG TGGAGACAAC
    CAAGGGGTAA AATTCCTGCG TCGCTTCAAA CAGCTGCTCA ATCCTGCTCA GGTCTTTGAC
    CTGGTCAACG GTGGACCTCA TTTAGGGCTT CGCTTGTTCC AAAAGTTTGA CAACTTCCGG
    ATCCTTGTGT GTGGTGGAGA TGGAAGCGTT GGCTGGGTGC TGTCTGAAAT CGATAAACTA
    AATCTTCATA AACAGTGCCA GCTGGGTGTT CTGCCGTTGG GCACCGGGAA TGACCTAGCA
    CGGGTGCTAG GTTGGGGGCC GTCATGTGAT GATGACACTC AGCTGCCTCA GATACTGGAG
    AAGCTGGAGC GAGCTAGCAC CAAAATGCTG GACAGGTGGA GTATAATGAC GTATGAGATT
    AAAATACCTC CCAAACACAG CTGTCCTGCT ACGCCTGAGG AGGCAGAGGA CGGCCAGTTA
    CAGATATCAG CTTATGAGGA TTCAGTGGCT GCTCATCTCA CAAAGATTCT GAACTCAGAT
    CAGCACTCAG TGGTCATATC TTCATCAAAA GTATTGTGTG AGACGGTGAA GGAATTTGTG
    TCAAGAGTTG GAAAGTGTTA CGAGAGAGGC AGCGACAGCT CAGAAGAATC TGATGCACTG
    GCTCTCAAAT GTGCAATGCT GAATGAGAAG CTGGAGTCAC TCCTGCAGAC GTTAAATTTG
    GAGGCTCAGG TCCTGTCGCC CACAGCCCTT ACCACACCAC CCATCGTGGA GGAGGAGGTG
    GAGGAGGAAG AGCTGGAGGA GGAAGACGAA GATCTGAGCG AAGAGTCTCT AACAGAGTTG
    AAGGAGAACG AGACGGAGAA AGGATCCGCT CATAAACTCT TCAGACCCAG AGAGCAGCTG
    GTGCTGAGGG CCAACAGCCT GAAGAAAGCC CTCAGACAGA TCATCGAGCA GGCTGAGAGA
    ATGGTGGATG AACAGAACGC ACACACAGAG GAGCAGGAGC TTCAGTCTCC TTCTGACTTC
    AGGAAGGACA GCGAAGAGGA AAACAAAGAC AACGAGAAGG ACGAGGACAC TAAAGAACTC
    GAAACTCTGC CTTTGGCAAA GTCTCCATGT TCTCCTCCAG AGAGACGCAT CAGTCGCAGC
    ACACAGTCCT GCGGATCCTT CTCCATCACT CCCTTCACCA CAAGCAAAGA GAACCTGCCC
    GTTCTCAACA CCCGCATCAT CTGTCCCGGT CTGCGGGCAG GTCTCGCTGC CTCCATCGCT
    GGAAGCTCTA TAATCAGTAA AATGCTGCTG GCCAACATTG ATCCGTTTGG TGCCACGCCA
    TTCATAGATC CTGACCTAGA TTCACTAGAG GGCTATTTGG AGAAGTGTGT GATGAATAAC
    TACTTTGGGA TCGGCTTGGA TGCAAAGATC TCACTGGAGT TCAACAACAA GCGTGAGGAG
    CATCCGGAGA AGTGCAGGAG TCGCACTAAA AACATGATGT GGTATGGTGT TCTGGGGACA
    AAAGAGTTGC TGCAGAGGAC TTATAAGAAC CTGGAACAGA AAGTTCAGCT GGAGTGTGAT
    GGCCAGTATA TCCCACTCCC AAGTCTCCAA GGAATTGCTG TGCTGAACAT CCCAAGCTAT
    GCTGGGGGAA CCAACTTCTG GGGCGGCACC AAAGAGGATG ATATATTTTG TGCCCCTTCA
    TTCGATGATA AAATTTTGGA AGTGGTCGCT GTGTTTGGTA GTATGCAGAT GGCTGTCTCA
    AGGGTGATTA AACTGCAGCA TCACCGTATT GCACAGTGTC GCACGGTGAA GATCACAATC
    CTGGGGGATG AAGGAGTGCC TGTCCAGGTG GATGGTGAAG CCTGGATTCA ACCTGCTGGA
    GTCATCAAGA TTCAGCACAA GAACCGGGCC CAGATGCTGA CAAGAGACCG GGCGTTTGAA
    AACACGTTGA AGTCTTGGGA GGATAAATTG AAATACGACA AGCCTCCGCT GCGGCCACAT
    CTGTACCCTC AGCAGTCTGT GGATCTGGCC ACAGAGGAGG AGGCCACGCT CATCCAGATG
    TGTGCCCGCG CTGCTGAGGA TCTCATCACC AGGATCTGTG AGGCTGCCAA AAACTACCAG
    CTCCTCGAGC AGGAGCTCGC CCACGCCGTC AACGCCTCCT CACATGCCAT TAACAAAACA
    CATCCCAAGT TCCCAGAGAG CCTGACAAGA AACACTGCCA TTGAGGTGGC CAGCACCGTG
    AAGGCCCTGC ACAATGAGAC AGAGTCTCTG TTAGTAGGAC GCGTGCCATT GCAACTGGAC
    CCTCCGCATG AAGATCTGCT GTCCACTGCG CTGCAGAACG TTGAGGTTGA ACTAGGCAAG
    CTGGTTGAGA TCCCTTGGCT CTACCACATC TTACAGCCCA ATGATGAGGA GGATCCCTCC
    CTTGAGTATG GAAAAAGAAA CAGTCGGAGT GGCATGTTCC GCATAGTGCC AAAGTTCAAG
    AAAGAGAAAG TGCCCAAGAA GTCTAGCCCA CAATCAGACG ATGTCCATGG ACATACGGAG
    GCAGTGACAC GCAGCCTGTG TGATGAAATA GAGCGCCATT CAGCATTTCA GAGGTGGGGT
    ACTGAGGAGG TGGCCATCTG GCTGGAGCAG CTCAGCTTGG GAGAGTACAA AGACACCTTC
    ATCCGCCACG ACATCCGCGG CTCCGAGCTC CTGCACCTGG AGAGGAGAGA CCTTAAGGAT
    CTGGGGATCT CGAAGGTGGG CCATATGAAG AGAATTCTCC AGGGAACTAA GGACTTGGCC
    AAGAGTTCGA TGGTGGACCT GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021334552.1
    CDS118..3441
    Translation

    Target ORF information:

    RefSeq Version XM_021478877.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio diacylglycerol kinase, eta (dgkh), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021478877.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAGGACT GGATCAGCTC GCTGAAATCG GTTCAGTCCA GAGAGCATTA CGAGACGGCA 
    CAGTTTAATG TGGAACATTT CTCAGGGATG CACAACTGGT ATGCCTGCTC CCACGCTCGC
    CCCACCTTCT GTAACGTCTG TCGGGACAGC CTCTCAGGGG TCACTTCTCA TGGCCTCTCC
    TGTGAAGTGT GCAAATTCAA AGCCCACAAA AGATGTGCAG TCAGGGCCAC TAACAACTGC
    AAATGGACAA CATTGGCCTC CATCGGGAAG GACATTATTG AAGACGAGGA TGGGATTGCA
    ATGCCGCACC AATGGCTGGA AGGCAACCTG CCGGTTAGTG CAAAGTGTGC TGTTTGCGAC
    AAAACATGTG GCAGCGTGCT GCGCTTGCAG GACTGGCGCT GTTTGTGGTG CAAGGCCATG
    GTGCACACGG CATGCATGGA TCTCTACCCC CGCAAATGTC CCCTCGGCCA GTGCAAAGTG
    TCCATCATCC CCCCCACTGC CCTTAACAGC ATCGACTCAG ACGGTTTTTG GAAGGCCACG
    TGCCCTCCAT CATGTGCAAG TCCGCTCCTC GTGTTTGTCA ACTCAAAAAG TGGAGACAAC
    CAAGGGGTAA AATTCCTGCG TCGCTTCAAA CAGCTGCTCA ATCCTGCTCA GGTCTTTGAC
    CTGGTCAACG GTGGACCTCA TTTAGGGCTT CGCTTGTTCC AAAAGTTTGA CAACTTCCGG
    ATCCTTGTGT GTGGTGGAGA TGGAAGCGTT GGCTGGGTGC TGTCTGAAAT CGATAAACTA
    AATCTTCATA AACAGTGCCA GCTGGGTGTT CTGCCGTTGG GCACCGGGAA TGACCTAGCA
    CGGGTGCTAG GTTGGGGGCC GTCATGTGAT GATGACACTC AGCTGCCTCA GATACTGGAG
    AAGCTGGAGC GAGCTAGCAC CAAAATGCTG GACAGGTGGA GTATAATGAC GTATGAGATT
    AAAATACCTC CCAAACACAG CTGTCCTGCT ACGCCTGAGG AGGCAGAGGA CGGCCAGTTA
    CAGATATCAG CTTATGAGGA TTCAGTGGCT GCTCATCTCA CAAAGATTCT GAACTCAGAT
    CAGCACTCAG TGGTCATATC TTCATCAAAA GTATTGTGTG AGACGGTGAA GGAATTTGTG
    TCAAGAGTTG GAAAGTGTTA CGAGAGAGGC AGCGACAGCT CAGAAGAATC TGATGCACTG
    GCTCTCAAAT GTGCAATGCT GAATGAGAAG CTGGAGTCAC TCCTGCAGAC GTTAAATTTG
    GAGGCTCAGG TCCTGTCGCC CACAGCCCTT ACCACACCAC CCATCGTGGA GGAGGAGGTG
    GAGGAGGAAG AGCTGGAGGA GGAAGACGAA GATCTGAGCG AAGAGTCTCT AACAGAGTTG
    AAGGAGAACG AGACGGAGAA AGGATCCGCT CATAAACTCT TCAGACCCAG AGAGCAGCTG
    GTGCTGAGGG CCAACAGCCT GAAGAAAGCC CTCAGACAGA TCATCGAGCA GGCTGAGAGA
    ATGGTGGATG AACAGAACGC ACACACAGAG GAGCAGGAGC TTCAGTCTCC TTCTGACTTC
    AGGAAGGACA GCGAAGAGGA AAACAAAGAC AACGAGAAGG ACGAGGACAC TAAAGAACTC
    GAAACTCTGC CTTTGGCAAA GTCTCCATGT TCTCCTCCAG AGAGACGCAT CAGTCGCAGC
    ACACAGTCCT GCGGATCCTT CTCCATCACT CCCTTCACCA CAAGCAAAGA GAACCTGCCC
    GTTCTCAACA CCCGCATCAT CTGTCCCGGT CTGCGGGCAG GTCTCGCTGC CTCCATCGCT
    GGAAGCTCTA TAATCAGTAA AATGCTGCTG GCCAACATTG ATCCGTTTGG TGCCACGCCA
    TTCATAGATC CTGACCTAGA TTCACTAGAG GGCTATTTGG AGAAGTGTGT GATGAATAAC
    TACTTTGGGA TCGGCTTGGA TGCAAAGATC TCACTGGAGT TCAACAACAA GCGTGAGGAG
    CATCCGGAGA AGTGCAGGAG TCGCACTAAA AACATGATGT GGTATGGTGT TCTGGGGACA
    AAAGAGTTGC TGCAGAGGAC TTATAAGAAC CTGGAACAGA AAGTTCAGCT GGAGTGTGAT
    GGCCAGTATA TCCCACTCCC AAGTCTCCAA GGAATTGCTG TGCTGAACAT CCCAAGCTAT
    GCTGGGGGAA CCAACTTCTG GGGCGGCACC AAAGAGGATG ATATATTTTG TGCCCCTTCA
    TTCGATGATA AAATTTTGGA AGTGGTCGCT GTGTTTGGTA GTATGCAGAT GGCTGTCTCA
    AGGGTGATTA AACTGCAGCA TCACCGTATT GCACAGTGTC GCACGGTGAA GATCACAATC
    CTGGGGGATG AAGGAGTGCC TGTCCAGGTG GATGGTGAAG CCTGGATTCA ACCTGCTGGA
    GTCATCAAGA TTCAGCACAA GAACCGGGCC CAGATGCTGA CAAGAGACCG GGCGTTTGAA
    AACACGTTGA AGTCTTGGGA GGATAAATTG AAATACGACA AGCCTCCGCT GCGGCCACAT
    CTGTACCCTC AGCAGTCTGT GGATCTGGCC ACAGAGGAGG AGGCCACGCT CATCCAGATG
    TGTGCCCGCG CTGCTGAGGA TCTCATCACC AGGATCTGTG AGGCTGCCAA AAACTACCAG
    CTCCTCGAGC AGGAGCTCGC CCACGCCGTC AACGCCTCCT CACATGCCAT TAACAAAACA
    CATCCCAAGT TCCCAGAGAG CCTGACAAGA AACACTGCCA TTGAGGTGGC CAGCACCGTG
    AAGGCCCTGC ACAATGAGAC AGAGTCTCTG TTAGTAGGAC GCGTGCCATT GCAACTGGAC
    CCTCCGCATG AAGATCTGCT GTCCACTGCG CTGCAGAACG TTGAGGTTGA ACTAGGCAAG
    CTGGTTGAGA TCCCTTGGCT CTACCACATC TTACAGCCCA ATGATGAGGA GGATCCCTCC
    CTTGAGTATG GAAAAAGAAA CAGTCGGAGT GGCATGTTCC GCATAGTGCC AAAGTTCAAG
    AAAGAGAAAG TGCCCAAGAA GTCTAGCCCA CAATCAGACG ATGTCCATGG ACATACGGAG
    GCAGTGACAC GCAGCCTGTG TGATGAAATA GAGCGCCATT CAGCATTTCA GAGGTGGGGT
    ACTGAGGAGG TGGCCATCTG GCTGGAGCAG CTCAGCTTGG GAGAGTACAA AGACACCTTC
    ATCCGCCACG ACATCCGCGG CTCCGAGCTC CTGCACCTGG AGAGGAGAGA CCTTAAGGAT
    CTGGGGATCT CGAAGGTGGG CCATATGAAG AGAATTCTCC AGGGAACTAA GGACTTGGCC
    AAGAGTTCGA TGGTGGACCT GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa15017
    Clone ID Related Accession (Same CDS sequence) XM_005167979.3 , XM_005167979.4
    Accession Version XM_005167979.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3774bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product diacylglycerol kinase eta isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005167979.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGACGGCCA AGAGAACCGC TTTCCAGCCG TTGTCCAGCT CCCGCATCCC AGCGGAGAGG 
    AAGGGAGCGG CTGAGCGCGT TCCTAACCCG GAGTGCTGTG ACTTTCCCCC GCAGGAGCCG
    GAGAAAGCAG CTGCGTCCGC CTCGGGGGTT CCCGCCGTGG GGGCTGTCGG GGGCTCGGGG
    CCGGTGGTGG ACGAGTCATC CGACAGCGAA GGAGAGCAGG AAGGGCCGCA GAAACTCATC
    AGGAAAGTGT CAACCTCCGG GCAAATACGA AGCAAGACAA GCGTGAAGGA AGGGCTTCTT
    CTGAAACAGA CAAGCTCGTT CCAGCGTTGG AAAAAACGTT ATTTCAAGCT ACGAGGACGG
    ACGCTCTACT ATGCTAAGGA TGCCAAGTCT CTTATTTTCG ATGAGGTGGA CCTTTCAGAT
    GCCAGTGTTG CCGAATCAAG TACAAAGAAT ATCAACAACA GTTTCACGGT AATTACACCG
    TTTCGAAGGC TTATCCTGTG CGCCGAGAAC AGGAAAGAAA TGGAGGACTG GATCAGCTCG
    CTGAAATCGG TTCAGTCCAG AGAGCATTAC GAGACGGCAC AGTTTAATGT GGAACATTTC
    TCAGGGATGC ACAACTGGTA TGCCTGCTCC CACGCTCGCC CCACCTTCTG TAACGTCTGT
    CGGGACAGCC TCTCAGGGGT CACTTCTCAT GGCCTCTCCT GTGAAGTGTG CAAATTCAAA
    GCCCACAAAA GATGTGCAGT CAGGGCCACT AACAACTGCA AATGGACAAC ATTGGCCTCC
    ATCGGGAAGG ACATTATTGA AGACGAGGAT GGGATTGCAA TGCCGCACCA ATGGCTGGAA
    GGCAACCTGC CGGTTAGTGC AAAGTGTGCT GTTTGCGACA AAACATGTGG CAGCGTGCTG
    CGCTTGCAGG ACTGGCGCTG TTTGTGGTGC AAGGCCATGG TGCACACGGC ATGCATGGAT
    CTCTACCCCC GCAAATGTCC CCTCGGCCAG TGCAAAGTGT CCATCATCCC CCCCACTGCC
    CTTAACAGCA TCGACTCAGA CGGTTTTTGG AAGGCCACGT GCCCTCCATC ATGTGCAAGT
    CCGCTCCTCG TGTTTGTCAA CTCAAAAAGT GGAGACAACC AAGGGGTAAA ATTCCTGCGT
    CGCTTCAAAC AGCTGCTCAA TCCTGCTCAG GTCTTTGACC TGGTCAACGG TGGACCTCAT
    TTAGGGCTTC GCTTGTTCCA AAAGTTTGAC AACTTCCGGA TCCTTGTGTG TGGTGGAGAT
    GGAAGCGTTG GCTGGGTGCT GTCTGAAATC GATAAACTAA ATCTTCATAA ACAGTGCCAG
    CTGGGTGTTC TGCCGTTGGG CACCGGGAAT GACCTAGCAC GGGTGCTAGG TTGGGGGCCG
    TCATGTGATG ATGACACTCA GCTGCCTCAG ATACTGGAGA AGCTGGAGCG AGCTAGCACC
    AAAATGCTGG ACAGGTGGAG TATAATGACG TATGAGATTA AAATACCTCC CAAACACAGC
    TGTCCTGCTA CGCCTGAGGA GGCAGAGGAC GGCCAGTTAC AGATATCAGC TTATGAGGAT
    TCAGTGGCTG CTCATCTCAC AAAGATTCTG AACTCAGATC AGCACTCAGT GGTCATATCT
    TCATCAAAAG TATTGTGTGA GACGGTGAAG GAATTTGTGT CAAGAGTTGG AAAGTGTTAC
    GAGAGAGGCA GCGACAGCTC AGAAGAATCT GATGCACTGG CTCTCAAATG TGCAATGCTG
    AATGAGAAGC TGGAGTCACT CCTGCAGACG TTAAATTTGG AGGCTCAGGT CCTGTCGCCC
    ACAGCCCTTA CCACACCACC CATCGTGGAG GAGGAGGTGG AGGAGGAAGA GCTGGAGGAG
    GAAGACGAAG ATCTGAGCGA AGAGTCTCTA ACAGAGTTGA AGGAGAACGA GACGGAGAAA
    GGATCCGCTC ATAAACTCTT CAGACCCAGA GAGCAGCTGG TGCTGAGGGC CAACAGCCTG
    AAGAAAGCCC TCAGACAGAT CATCGAGCAG GCTGAGAGAA TGGTGGATGA ACAGAACGCA
    CACACAGAGG AGCAGGAGCT TCAGTCTCCT TCTGACTTCA GGAAGGACAG CGAAGAGGAA
    AACAAAGACA ACGAGAAGGA CGAGGACACT AAAGAACTCG AAACTCTGCC TTTGGCAAAG
    TCTCCATGTT CTCCTCCAGA GAGACGCATC AGTCGCAGCA CACAGTCCTG CGGATCCTTC
    TCCATCACTC CCTTCACCAC AAGCAAAGAG AACCTGCCCG TTCTCAACAC CCGCATCATC
    TGTCCCGGTC TGCGGGCAGG TCTCGCTGCC TCCATCGCTG GAAGCTCTAT AATCAGTAAA
    ATGCTGCTGG CCAACATTGA TCCGTTTGGT GCCACGCCAT TCATAGATCC TGACCTAGAT
    TCACTAGAGG GCTATTTGGA GAAGTGTGTG ATGAATAACT ACTTTGGGAT CGGCTTGGAT
    GCAAAGATCT CACTGGAGTT CAACAACAAG CGTGAGGAGC ATCCGGAGAA GTGCAGGAGT
    CGCACTAAAA ACATGATGTG GTATGGTGTT CTGGGGACAA AAGAGTTGCT GCAGAGGACT
    TATAAGAACC TGGAACAGAA AGTTCAGCTG GAGTGTGATG GCCAGTATAT CCCACTCCCA
    AGTCTCCAAG GAATTGCTGT GCTGAACATC CCAAGCTATG CTGGGGGAAC CAACTTCTGG
    GGCGGCACCA AAGAGGATGA TATATTTTGT GCCCCTTCAT TCGATGATAA AATTTTGGAA
    GTGGTCGCTG TGTTTGGTAG TATGCAGATG GCTGTCTCAA GGGTGATTAA ACTGCAGCAT
    CACCGTATTG CACAGTGTCG CACGGTGAAG ATCACAATCC TGGGGGATGA AGGAGTGCCT
    GTCCAGGTGG ATGGTGAAGC CTGGATTCAA CCTGCTGGAG TCATCAAGAT TCAGCACAAG
    AACCGGGCCC AGATGCTGAC AAGAGACCGG GCGTTTGAAA ACACGTTGAA GTCTTGGGAG
    GATAAATTGA AATACGACAA GCCTCCGCTG CGGCCACATC TGTACCCTCA GCAGTCTGTG
    GATCTGGCCA CAGAGGAGGA GGCCACGCTC ATCCAGATGT GTGCCCGCGC TGCTGAGGAT
    CTCATCACCA GGATCTGTGA GGCTGCCAAA AACTACCAGC TCCTCGAGCA GGAGCTCGCC
    CACGCCGTCA ACGCCTCCTC ACATGCCATT AACAAAACAC ATCCCAAGTT CCCAGAGAGC
    CTGACAAGAA ACACTGCCAT TGAGGTGGCC AGCACCGTGA AGGCCCTGCA CAATGAGACA
    GAGTCTCTGT TAGTAGGACG CGTGCCATTG CAACTGGACC CTCCGCATGA AGATCTGCTG
    TCCACTGCGC TGCAGAACGT TGAGGTTGAA CTAGGCAAGC TGGTTGAGAT CCCTTGGCTC
    TACCACATCT TACAGCCCAA TGATGAGGAG GATCCCTCCC TTGAGTATGG AAAAAGAAAC
    AGTCGGAGTG GCATGTTCCG CATAGTGCCA AAGTTCAAGA AAGAGAAAGT GCCCAAGAAG
    TCTAGCCCAC AATCAGTTCA GAGGTGGGGT ACTGAGGAGG TGGCCATCTG GCTGGAGCAG
    CTCAGCTTGG GAGAGTACAA AGACACCTTC ATCCGCCACG ACATCCGCGG CTCCGAGCTC
    CTGCACCTGG AGAGGAGAGA CCTTAAGGAT CTGGGGATCT CGAAGGTGGG CCATATGAAG
    AGAATTCTCC AGGGAACTAA GGACTTGGCC AAGAGTTCGA TGGTGGACCT GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005168036.1
    CDS649..4422
    Translation

    Target ORF information:

    RefSeq Version XM_005167979.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio diacylglycerol kinase, eta (dgkh), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005167979.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGACGGCCA AGAGAACCGC TTTCCAGCCG TTGTCCAGCT CCCGCATCCC AGCGGAGAGG 
    AAGGGAGCGG CTGAGCGCGT TCCTAACCCG GAGTGCTGTG ACTTTCCCCC GCAGGAGCCG
    GAGAAAGCAG CTGCGTCCGC CTCGGGGGTT CCCGCCGTGG GGGCTGTCGG GGGCTCGGGG
    CCGGTGGTGG ACGAGTCATC CGACAGCGAA GGAGAGCAGG AAGGGCCGCA GAAACTCATC
    AGGAAAGTGT CAACCTCCGG GCAAATACGA AGCAAGACAA GCGTGAAGGA AGGGCTTCTT
    CTGAAACAGA CAAGCTCGTT CCAGCGTTGG AAAAAACGTT ATTTCAAGCT ACGAGGACGG
    ACGCTCTACT ATGCTAAGGA TGCCAAGTCT CTTATTTTCG ATGAGGTGGA CCTTTCAGAT
    GCCAGTGTTG CCGAATCAAG TACAAAGAAT ATCAACAACA GTTTCACGGT AATTACACCG
    TTTCGAAGGC TTATCCTGTG CGCCGAGAAC AGGAAAGAAA TGGAGGACTG GATCAGCTCG
    CTGAAATCGG TTCAGTCCAG AGAGCATTAC GAGACGGCAC AGTTTAATGT GGAACATTTC
    TCAGGGATGC ACAACTGGTA TGCCTGCTCC CACGCTCGCC CCACCTTCTG TAACGTCTGT
    CGGGACAGCC TCTCAGGGGT CACTTCTCAT GGCCTCTCCT GTGAAGTGTG CAAATTCAAA
    GCCCACAAAA GATGTGCAGT CAGGGCCACT AACAACTGCA AATGGACAAC ATTGGCCTCC
    ATCGGGAAGG ACATTATTGA AGACGAGGAT GGGATTGCAA TGCCGCACCA ATGGCTGGAA
    GGCAACCTGC CGGTTAGTGC AAAGTGTGCT GTTTGCGACA AAACATGTGG CAGCGTGCTG
    CGCTTGCAGG ACTGGCGCTG TTTGTGGTGC AAGGCCATGG TGCACACGGC ATGCATGGAT
    CTCTACCCCC GCAAATGTCC CCTCGGCCAG TGCAAAGTGT CCATCATCCC CCCCACTGCC
    CTTAACAGCA TCGACTCAGA CGGTTTTTGG AAGGCCACGT GCCCTCCATC ATGTGCAAGT
    CCGCTCCTCG TGTTTGTCAA CTCAAAAAGT GGAGACAACC AAGGGGTAAA ATTCCTGCGT
    CGCTTCAAAC AGCTGCTCAA TCCTGCTCAG GTCTTTGACC TGGTCAACGG TGGACCTCAT
    TTAGGGCTTC GCTTGTTCCA AAAGTTTGAC AACTTCCGGA TCCTTGTGTG TGGTGGAGAT
    GGAAGCGTTG GCTGGGTGCT GTCTGAAATC GATAAACTAA ATCTTCATAA ACAGTGCCAG
    CTGGGTGTTC TGCCGTTGGG CACCGGGAAT GACCTAGCAC GGGTGCTAGG TTGGGGGCCG
    TCATGTGATG ATGACACTCA GCTGCCTCAG ATACTGGAGA AGCTGGAGCG AGCTAGCACC
    AAAATGCTGG ACAGGTGGAG TATAATGACG TATGAGATTA AAATACCTCC CAAACACAGC
    TGTCCTGCTA CGCCTGAGGA GGCAGAGGAC GGCCAGTTAC AGATATCAGC TTATGAGGAT
    TCAGTGGCTG CTCATCTCAC AAAGATTCTG AACTCAGATC AGCACTCAGT GGTCATATCT
    TCATCAAAAG TATTGTGTGA GACGGTGAAG GAATTTGTGT CAAGAGTTGG AAAGTGTTAC
    GAGAGAGGCA GCGACAGCTC AGAAGAATCT GATGCACTGG CTCTCAAATG TGCAATGCTG
    AATGAGAAGC TGGAGTCACT CCTGCAGACG TTAAATTTGG AGGCTCAGGT CCTGTCGCCC
    ACAGCCCTTA CCACACCACC CATCGTGGAG GAGGAGGTGG AGGAGGAAGA GCTGGAGGAG
    GAAGACGAAG ATCTGAGCGA AGAGTCTCTA ACAGAGTTGA AGGAGAACGA GACGGAGAAA
    GGATCCGCTC ATAAACTCTT CAGACCCAGA GAGCAGCTGG TGCTGAGGGC CAACAGCCTG
    AAGAAAGCCC TCAGACAGAT CATCGAGCAG GCTGAGAGAA TGGTGGATGA ACAGAACGCA
    CACACAGAGG AGCAGGAGCT TCAGTCTCCT TCTGACTTCA GGAAGGACAG CGAAGAGGAA
    AACAAAGACA ACGAGAAGGA CGAGGACACT AAAGAACTCG AAACTCTGCC TTTGGCAAAG
    TCTCCATGTT CTCCTCCAGA GAGACGCATC AGTCGCAGCA CACAGTCCTG CGGATCCTTC
    TCCATCACTC CCTTCACCAC AAGCAAAGAG AACCTGCCCG TTCTCAACAC CCGCATCATC
    TGTCCCGGTC TGCGGGCAGG TCTCGCTGCC TCCATCGCTG GAAGCTCTAT AATCAGTAAA
    ATGCTGCTGG CCAACATTGA TCCGTTTGGT GCCACGCCAT TCATAGATCC TGACCTAGAT
    TCACTAGAGG GCTATTTGGA GAAGTGTGTG ATGAATAACT ACTTTGGGAT CGGCTTGGAT
    GCAAAGATCT CACTGGAGTT CAACAACAAG CGTGAGGAGC ATCCGGAGAA GTGCAGGAGT
    CGCACTAAAA ACATGATGTG GTATGGTGTT CTGGGGACAA AAGAGTTGCT GCAGAGGACT
    TATAAGAACC TGGAACAGAA AGTTCAGCTG GAGTGTGATG GCCAGTATAT CCCACTCCCA
    AGTCTCCAAG GAATTGCTGT GCTGAACATC CCAAGCTATG CTGGGGGAAC CAACTTCTGG
    GGCGGCACCA AAGAGGATGA TATATTTTGT GCCCCTTCAT TCGATGATAA AATTTTGGAA
    GTGGTCGCTG TGTTTGGTAG TATGCAGATG GCTGTCTCAA GGGTGATTAA ACTGCAGCAT
    CACCGTATTG CACAGTGTCG CACGGTGAAG ATCACAATCC TGGGGGATGA AGGAGTGCCT
    GTCCAGGTGG ATGGTGAAGC CTGGATTCAA CCTGCTGGAG TCATCAAGAT TCAGCACAAG
    AACCGGGCCC AGATGCTGAC AAGAGACCGG GCGTTTGAAA ACACGTTGAA GTCTTGGGAG
    GATAAATTGA AATACGACAA GCCTCCGCTG CGGCCACATC TGTACCCTCA GCAGTCTGTG
    GATCTGGCCA CAGAGGAGGA GGCCACGCTC ATCCAGATGT GTGCCCGCGC TGCTGAGGAT
    CTCATCACCA GGATCTGTGA GGCTGCCAAA AACTACCAGC TCCTCGAGCA GGAGCTCGCC
    CACGCCGTCA ACGCCTCCTC ACATGCCATT AACAAAACAC ATCCCAAGTT CCCAGAGAGC
    CTGACAAGAA ACACTGCCAT TGAGGTGGCC AGCACCGTGA AGGCCCTGCA CAATGAGACA
    GAGTCTCTGT TAGTAGGACG CGTGCCATTG CAACTGGACC CTCCGCATGA AGATCTGCTG
    TCCACTGCGC TGCAGAACGT TGAGGTTGAA CTAGGCAAGC TGGTTGAGAT CCCTTGGCTC
    TACCACATCT TACAGCCCAA TGATGAGGAG GATCCCTCCC TTGAGTATGG AAAAAGAAAC
    AGTCGGAGTG GCATGTTCCG CATAGTGCCA AAGTTCAAGA AAGAGAAAGT GCCCAAGAAG
    TCTAGCCCAC AATCAGTTCA GAGGTGGGGT ACTGAGGAGG TGGCCATCTG GCTGGAGCAG
    CTCAGCTTGG GAGAGTACAA AGACACCTTC ATCCGCCACG ACATCCGCGG CTCCGAGCTC
    CTGCACCTGG AGAGGAGAGA CCTTAAGGAT CTGGGGATCT CGAAGGTGGG CCATATGAAG
    AGAATTCTCC AGGGAACTAA GGACTTGGCC AAGAGTTCGA TGGTGGACCT GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa15017
    Clone ID Related Accession (Same CDS sequence) XM_005167979.3 , XM_005167979.4
    Accession Version XM_005167979.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3774bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product diacylglycerol kinase eta isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005167979.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGACGGCCA AGAGAACCGC TTTCCAGCCG TTGTCCAGCT CCCGCATCCC AGCGGAGAGG 
    AAGGGAGCGG CTGAGCGCGT TCCTAACCCG GAGTGCTGTG ACTTTCCCCC GCAGGAGCCG
    GAGAAAGCAG CTGCGTCCGC CTCGGGGGTT CCCGCCGTGG GGGCTGTCGG GGGCTCGGGG
    CCGGTGGTGG ACGAGTCATC CGACAGCGAA GGAGAGCAGG AAGGGCCGCA GAAACTCATC
    AGGAAAGTGT CAACCTCCGG GCAAATACGA AGCAAGACAA GCGTGAAGGA AGGGCTTCTT
    CTGAAACAGA CAAGCTCGTT CCAGCGTTGG AAAAAACGTT ATTTCAAGCT ACGAGGACGG
    ACGCTCTACT ATGCTAAGGA TGCCAAGTCT CTTATTTTCG ATGAGGTGGA CCTTTCAGAT
    GCCAGTGTTG CCGAATCAAG TACAAAGAAT ATCAACAACA GTTTCACGGT AATTACACCG
    TTTCGAAGGC TTATCCTGTG CGCCGAGAAC AGGAAAGAAA TGGAGGACTG GATCAGCTCG
    CTGAAATCGG TTCAGTCCAG AGAGCATTAC GAGACGGCAC AGTTTAATGT GGAACATTTC
    TCAGGGATGC ACAACTGGTA TGCCTGCTCC CACGCTCGCC CCACCTTCTG TAACGTCTGT
    CGGGACAGCC TCTCAGGGGT CACTTCTCAT GGCCTCTCCT GTGAAGTGTG CAAATTCAAA
    GCCCACAAAA GATGTGCAGT CAGGGCCACT AACAACTGCA AATGGACAAC ATTGGCCTCC
    ATCGGGAAGG ACATTATTGA AGACGAGGAT GGGATTGCAA TGCCGCACCA ATGGCTGGAA
    GGCAACCTGC CGGTTAGTGC AAAGTGTGCT GTTTGCGACA AAACATGTGG CAGCGTGCTG
    CGCTTGCAGG ACTGGCGCTG TTTGTGGTGC AAGGCCATGG TGCACACGGC ATGCATGGAT
    CTCTACCCCC GCAAATGTCC CCTCGGCCAG TGCAAAGTGT CCATCATCCC CCCCACTGCC
    CTTAACAGCA TCGACTCAGA CGGTTTTTGG AAGGCCACGT GCCCTCCATC ATGTGCAAGT
    CCGCTCCTCG TGTTTGTCAA CTCAAAAAGT GGAGACAACC AAGGGGTAAA ATTCCTGCGT
    CGCTTCAAAC AGCTGCTCAA TCCTGCTCAG GTCTTTGACC TGGTCAACGG TGGACCTCAT
    TTAGGGCTTC GCTTGTTCCA AAAGTTTGAC AACTTCCGGA TCCTTGTGTG TGGTGGAGAT
    GGAAGCGTTG GCTGGGTGCT GTCTGAAATC GATAAACTAA ATCTTCATAA ACAGTGCCAG
    CTGGGTGTTC TGCCGTTGGG CACCGGGAAT GACCTAGCAC GGGTGCTAGG TTGGGGGCCG
    TCATGTGATG ATGACACTCA GCTGCCTCAG ATACTGGAGA AGCTGGAGCG AGCTAGCACC
    AAAATGCTGG ACAGGTGGAG TATAATGACG TATGAGATTA AAATACCTCC CAAACACAGC
    TGTCCTGCTA CGCCTGAGGA GGCAGAGGAC GGCCAGTTAC AGATATCAGC TTATGAGGAT
    TCAGTGGCTG CTCATCTCAC AAAGATTCTG AACTCAGATC AGCACTCAGT GGTCATATCT
    TCATCAAAAG TATTGTGTGA GACGGTGAAG GAATTTGTGT CAAGAGTTGG AAAGTGTTAC
    GAGAGAGGCA GCGACAGCTC AGAAGAATCT GATGCACTGG CTCTCAAATG TGCAATGCTG
    AATGAGAAGC TGGAGTCACT CCTGCAGACG TTAAATTTGG AGGCTCAGGT CCTGTCGCCC
    ACAGCCCTTA CCACACCACC CATCGTGGAG GAGGAGGTGG AGGAGGAAGA GCTGGAGGAG
    GAAGACGAAG ATCTGAGCGA AGAGTCTCTA ACAGAGTTGA AGGAGAACGA GACGGAGAAA
    GGATCCGCTC ATAAACTCTT CAGACCCAGA GAGCAGCTGG TGCTGAGGGC CAACAGCCTG
    AAGAAAGCCC TCAGACAGAT CATCGAGCAG GCTGAGAGAA TGGTGGATGA ACAGAACGCA
    CACACAGAGG AGCAGGAGCT TCAGTCTCCT TCTGACTTCA GGAAGGACAG CGAAGAGGAA
    AACAAAGACA ACGAGAAGGA CGAGGACACT AAAGAACTCG AAACTCTGCC TTTGGCAAAG
    TCTCCATGTT CTCCTCCAGA GAGACGCATC AGTCGCAGCA CACAGTCCTG CGGATCCTTC
    TCCATCACTC CCTTCACCAC AAGCAAAGAG AACCTGCCCG TTCTCAACAC CCGCATCATC
    TGTCCCGGTC TGCGGGCAGG TCTCGCTGCC TCCATCGCTG GAAGCTCTAT AATCAGTAAA
    ATGCTGCTGG CCAACATTGA TCCGTTTGGT GCCACGCCAT TCATAGATCC TGACCTAGAT
    TCACTAGAGG GCTATTTGGA GAAGTGTGTG ATGAATAACT ACTTTGGGAT CGGCTTGGAT
    GCAAAGATCT CACTGGAGTT CAACAACAAG CGTGAGGAGC ATCCGGAGAA GTGCAGGAGT
    CGCACTAAAA ACATGATGTG GTATGGTGTT CTGGGGACAA AAGAGTTGCT GCAGAGGACT
    TATAAGAACC TGGAACAGAA AGTTCAGCTG GAGTGTGATG GCCAGTATAT CCCACTCCCA
    AGTCTCCAAG GAATTGCTGT GCTGAACATC CCAAGCTATG CTGGGGGAAC CAACTTCTGG
    GGCGGCACCA AAGAGGATGA TATATTTTGT GCCCCTTCAT TCGATGATAA AATTTTGGAA
    GTGGTCGCTG TGTTTGGTAG TATGCAGATG GCTGTCTCAA GGGTGATTAA ACTGCAGCAT
    CACCGTATTG CACAGTGTCG CACGGTGAAG ATCACAATCC TGGGGGATGA AGGAGTGCCT
    GTCCAGGTGG ATGGTGAAGC CTGGATTCAA CCTGCTGGAG TCATCAAGAT TCAGCACAAG
    AACCGGGCCC AGATGCTGAC AAGAGACCGG GCGTTTGAAA ACACGTTGAA GTCTTGGGAG
    GATAAATTGA AATACGACAA GCCTCCGCTG CGGCCACATC TGTACCCTCA GCAGTCTGTG
    GATCTGGCCA CAGAGGAGGA GGCCACGCTC ATCCAGATGT GTGCCCGCGC TGCTGAGGAT
    CTCATCACCA GGATCTGTGA GGCTGCCAAA AACTACCAGC TCCTCGAGCA GGAGCTCGCC
    CACGCCGTCA ACGCCTCCTC ACATGCCATT AACAAAACAC ATCCCAAGTT CCCAGAGAGC
    CTGACAAGAA ACACTGCCAT TGAGGTGGCC AGCACCGTGA AGGCCCTGCA CAATGAGACA
    GAGTCTCTGT TAGTAGGACG CGTGCCATTG CAACTGGACC CTCCGCATGA AGATCTGCTG
    TCCACTGCGC TGCAGAACGT TGAGGTTGAA CTAGGCAAGC TGGTTGAGAT CCCTTGGCTC
    TACCACATCT TACAGCCCAA TGATGAGGAG GATCCCTCCC TTGAGTATGG AAAAAGAAAC
    AGTCGGAGTG GCATGTTCCG CATAGTGCCA AAGTTCAAGA AAGAGAAAGT GCCCAAGAAG
    TCTAGCCCAC AATCAGTTCA GAGGTGGGGT ACTGAGGAGG TGGCCATCTG GCTGGAGCAG
    CTCAGCTTGG GAGAGTACAA AGACACCTTC ATCCGCCACG ACATCCGCGG CTCCGAGCTC
    CTGCACCTGG AGAGGAGAGA CCTTAAGGAT CTGGGGATCT CGAAGGTGGG CCATATGAAG
    AGAATTCTCC AGGGAACTAA GGACTTGGCC AAGAGTTCGA TGGTGGACCT GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005168036.1
    CDS803..4576
    Translation

    Target ORF information:

    RefSeq Version XM_005167979.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio diacylglycerol kinase, eta (dgkh), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005167979.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGACGGCCA AGAGAACCGC TTTCCAGCCG TTGTCCAGCT CCCGCATCCC AGCGGAGAGG 
    AAGGGAGCGG CTGAGCGCGT TCCTAACCCG GAGTGCTGTG ACTTTCCCCC GCAGGAGCCG
    GAGAAAGCAG CTGCGTCCGC CTCGGGGGTT CCCGCCGTGG GGGCTGTCGG GGGCTCGGGG
    CCGGTGGTGG ACGAGTCATC CGACAGCGAA GGAGAGCAGG AAGGGCCGCA GAAACTCATC
    AGGAAAGTGT CAACCTCCGG GCAAATACGA AGCAAGACAA GCGTGAAGGA AGGGCTTCTT
    CTGAAACAGA CAAGCTCGTT CCAGCGTTGG AAAAAACGTT ATTTCAAGCT ACGAGGACGG
    ACGCTCTACT ATGCTAAGGA TGCCAAGTCT CTTATTTTCG ATGAGGTGGA CCTTTCAGAT
    GCCAGTGTTG CCGAATCAAG TACAAAGAAT ATCAACAACA GTTTCACGGT AATTACACCG
    TTTCGAAGGC TTATCCTGTG CGCCGAGAAC AGGAAAGAAA TGGAGGACTG GATCAGCTCG
    CTGAAATCGG TTCAGTCCAG AGAGCATTAC GAGACGGCAC AGTTTAATGT GGAACATTTC
    TCAGGGATGC ACAACTGGTA TGCCTGCTCC CACGCTCGCC CCACCTTCTG TAACGTCTGT
    CGGGACAGCC TCTCAGGGGT CACTTCTCAT GGCCTCTCCT GTGAAGTGTG CAAATTCAAA
    GCCCACAAAA GATGTGCAGT CAGGGCCACT AACAACTGCA AATGGACAAC ATTGGCCTCC
    ATCGGGAAGG ACATTATTGA AGACGAGGAT GGGATTGCAA TGCCGCACCA ATGGCTGGAA
    GGCAACCTGC CGGTTAGTGC AAAGTGTGCT GTTTGCGACA AAACATGTGG CAGCGTGCTG
    CGCTTGCAGG ACTGGCGCTG TTTGTGGTGC AAGGCCATGG TGCACACGGC ATGCATGGAT
    CTCTACCCCC GCAAATGTCC CCTCGGCCAG TGCAAAGTGT CCATCATCCC CCCCACTGCC
    CTTAACAGCA TCGACTCAGA CGGTTTTTGG AAGGCCACGT GCCCTCCATC ATGTGCAAGT
    CCGCTCCTCG TGTTTGTCAA CTCAAAAAGT GGAGACAACC AAGGGGTAAA ATTCCTGCGT
    CGCTTCAAAC AGCTGCTCAA TCCTGCTCAG GTCTTTGACC TGGTCAACGG TGGACCTCAT
    TTAGGGCTTC GCTTGTTCCA AAAGTTTGAC AACTTCCGGA TCCTTGTGTG TGGTGGAGAT
    GGAAGCGTTG GCTGGGTGCT GTCTGAAATC GATAAACTAA ATCTTCATAA ACAGTGCCAG
    CTGGGTGTTC TGCCGTTGGG CACCGGGAAT GACCTAGCAC GGGTGCTAGG TTGGGGGCCG
    TCATGTGATG ATGACACTCA GCTGCCTCAG ATACTGGAGA AGCTGGAGCG AGCTAGCACC
    AAAATGCTGG ACAGGTGGAG TATAATGACG TATGAGATTA AAATACCTCC CAAACACAGC
    TGTCCTGCTA CGCCTGAGGA GGCAGAGGAC GGCCAGTTAC AGATATCAGC TTATGAGGAT
    TCAGTGGCTG CTCATCTCAC AAAGATTCTG AACTCAGATC AGCACTCAGT GGTCATATCT
    TCATCAAAAG TATTGTGTGA GACGGTGAAG GAATTTGTGT CAAGAGTTGG AAAGTGTTAC
    GAGAGAGGCA GCGACAGCTC AGAAGAATCT GATGCACTGG CTCTCAAATG TGCAATGCTG
    AATGAGAAGC TGGAGTCACT CCTGCAGACG TTAAATTTGG AGGCTCAGGT CCTGTCGCCC
    ACAGCCCTTA CCACACCACC CATCGTGGAG GAGGAGGTGG AGGAGGAAGA GCTGGAGGAG
    GAAGACGAAG ATCTGAGCGA AGAGTCTCTA ACAGAGTTGA AGGAGAACGA GACGGAGAAA
    GGATCCGCTC ATAAACTCTT CAGACCCAGA GAGCAGCTGG TGCTGAGGGC CAACAGCCTG
    AAGAAAGCCC TCAGACAGAT CATCGAGCAG GCTGAGAGAA TGGTGGATGA ACAGAACGCA
    CACACAGAGG AGCAGGAGCT TCAGTCTCCT TCTGACTTCA GGAAGGACAG CGAAGAGGAA
    AACAAAGACA ACGAGAAGGA CGAGGACACT AAAGAACTCG AAACTCTGCC TTTGGCAAAG
    TCTCCATGTT CTCCTCCAGA GAGACGCATC AGTCGCAGCA CACAGTCCTG CGGATCCTTC
    TCCATCACTC CCTTCACCAC AAGCAAAGAG AACCTGCCCG TTCTCAACAC CCGCATCATC
    TGTCCCGGTC TGCGGGCAGG TCTCGCTGCC TCCATCGCTG GAAGCTCTAT AATCAGTAAA
    ATGCTGCTGG CCAACATTGA TCCGTTTGGT GCCACGCCAT TCATAGATCC TGACCTAGAT
    TCACTAGAGG GCTATTTGGA GAAGTGTGTG ATGAATAACT ACTTTGGGAT CGGCTTGGAT
    GCAAAGATCT CACTGGAGTT CAACAACAAG CGTGAGGAGC ATCCGGAGAA GTGCAGGAGT
    CGCACTAAAA ACATGATGTG GTATGGTGTT CTGGGGACAA AAGAGTTGCT GCAGAGGACT
    TATAAGAACC TGGAACAGAA AGTTCAGCTG GAGTGTGATG GCCAGTATAT CCCACTCCCA
    AGTCTCCAAG GAATTGCTGT GCTGAACATC CCAAGCTATG CTGGGGGAAC CAACTTCTGG
    GGCGGCACCA AAGAGGATGA TATATTTTGT GCCCCTTCAT TCGATGATAA AATTTTGGAA
    GTGGTCGCTG TGTTTGGTAG TATGCAGATG GCTGTCTCAA GGGTGATTAA ACTGCAGCAT
    CACCGTATTG CACAGTGTCG CACGGTGAAG ATCACAATCC TGGGGGATGA AGGAGTGCCT
    GTCCAGGTGG ATGGTGAAGC CTGGATTCAA CCTGCTGGAG TCATCAAGAT TCAGCACAAG
    AACCGGGCCC AGATGCTGAC AAGAGACCGG GCGTTTGAAA ACACGTTGAA GTCTTGGGAG
    GATAAATTGA AATACGACAA GCCTCCGCTG CGGCCACATC TGTACCCTCA GCAGTCTGTG
    GATCTGGCCA CAGAGGAGGA GGCCACGCTC ATCCAGATGT GTGCCCGCGC TGCTGAGGAT
    CTCATCACCA GGATCTGTGA GGCTGCCAAA AACTACCAGC TCCTCGAGCA GGAGCTCGCC
    CACGCCGTCA ACGCCTCCTC ACATGCCATT AACAAAACAC ATCCCAAGTT CCCAGAGAGC
    CTGACAAGAA ACACTGCCAT TGAGGTGGCC AGCACCGTGA AGGCCCTGCA CAATGAGACA
    GAGTCTCTGT TAGTAGGACG CGTGCCATTG CAACTGGACC CTCCGCATGA AGATCTGCTG
    TCCACTGCGC TGCAGAACGT TGAGGTTGAA CTAGGCAAGC TGGTTGAGAT CCCTTGGCTC
    TACCACATCT TACAGCCCAA TGATGAGGAG GATCCCTCCC TTGAGTATGG AAAAAGAAAC
    AGTCGGAGTG GCATGTTCCG CATAGTGCCA AAGTTCAAGA AAGAGAAAGT GCCCAAGAAG
    TCTAGCCCAC AATCAGTTCA GAGGTGGGGT ACTGAGGAGG TGGCCATCTG GCTGGAGCAG
    CTCAGCTTGG GAGAGTACAA AGACACCTTC ATCCGCCACG ACATCCGCGG CTCCGAGCTC
    CTGCACCTGG AGAGGAGAGA CCTTAAGGAT CTGGGGATCT CGAAGGTGGG CCATATGAAG
    AGAATTCTCC AGGGAACTAA GGACTTGGCC AAGAGTTCGA TGGTGGACCT GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.