KIF3B cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	KIF3B
Entrez Gene ID	100101641
Full Name	kinesin family member 3B
Synonyms	zgc:165446
General protein information	Preferred Names kinesin family member 3B Names kinesin-like protein KIF3B
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009296629.2	XP_009294904.1	kinesin-like protein KIF3B isoform X1
NM_001100145.1	NP_001093615.1	kinesin-like protein KIF3B
XM_005174101.4	XP_005174158.1	kinesin-like protein KIF3B isoform X1
XM_005174101.3	XP_005174158.1	kinesin-like protein KIF3B isoform X2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Kif3b	NP_032470.3
Xenopus tropicalis (tropical clawed frog)	kif3b	XP_004918676.1
Danio rerio (zebrafish)	kif3b	NP_001093615.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010396	XP_311552.4
Homo sapiens (human)	KIF3B	NP_004789.1
	KIF3B	XP_001108420.1
Bos taurus (cattle)	KIF3B	NP_001104258.1
Gallus gallus (chicken)	KIF3B	NP_001012852.1
Caenorhabditis elegans (roundworm)	klp-11	NP_741473.1
Pan troglodytes (chimpanzee)	KIF3B	XP_001155482.1
Canis lupus familiaris (dog)	KIF3B	XP_005634978.1
Rattus norvegicus (Norway rat)	Kif3b	NP_001099999.1
Drosophila melanogaster (fruit fly)	Klp68D	NP_001261726.1

Gene Ontology
Bibliography

Related articles in PubMed

The cytoplasmic tail of rhodopsin triggers rapid rod degeneration in kinesin-2 mutants.
Feng D, Chen Z, Yang K, Miao S, Xu B, Kang Y, Xie H, Zhao C
The Journal of biological chemistry292(42)17375-17386(2017 10)

Function-driven discovery of disease genes in zebrafish using an integrated genomics big data resource.
Shim H, Kim JH, Kim CY, Hwang S, Kim H, Yang S, Lee JE, Lee I
Nucleic acids research44(20)9611-9623(2016 Nov)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Differential roles for 3-OSTs in the regulation of cilia length and motility.
Neugebauer JM, Cadwallader AB, Amack JD, Bisgrove BW, Yost HJ
Development (Cambridge, England)140(18)3892-902(2013 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Knock-down of KIF3B Causes Early Embryonic Defects.
Title: Different roles for KIF17 and kinesin II in photoreceptor development and maintenance.

The following KIF3B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KIF3B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa02308	NM_001100145.1 Latest version!	Danio rerio kinesin family member 3B (kif3b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa27402	XM_009296629.2 Latest version!	Danio rerio kinesin family member 3B (kif3b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa27403	XM_005174101.4 Latest version!	Danio rerio kinesin family member 3B (kif3b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
ODa27403	XM_005174101.3	Danio rerio kinesin family member 3B (kif3b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

KIF3B ( NM_001100145.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001093615.1 Danio rerio kinesin family member 3B (kif3b), mRNA.

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CloneID	ODa02308
Clone ID Related Accession (Same CDS sequence)	NM_001100145.1
Accession Version	NM_001100145.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-12
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF3B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC146722.1. ##Evidence-Data-START## Transcript exon combination :: BC146722.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTCTAAAA GCAAGAGCTC TGAATCTGTT AAGGTTGTGG TGCGTTGTCG CCCCATGAAT 
GAGAAAGAGC GAGTCGCTAA CTTTAATAGA GTGGTATCTG TGGATGTGAA GTTAGGGCAA 
GTGGCAGTGT GCAACCCTCG AGGTGCATCT TCCCATGAGC ACCCAAAAGT GTTCACTTTT 
GATTCTGTCT ATGACTGGAA TTCAAAGCAG ATGGAGCTCT ACGACGAGAC TTTCCGCCCG 
TTAGTGGACT CTGTACTGTT TGGCTTCAAT GGGACCATCT TTGCATATGG CCAGACTGGC 
ACTGGAAAGA CTTACACAAT GGAAGGGGTA CGAAACGACC CGGAAAGAAG GGGTGTGATC 
CCAAACTCAT TTGAACACAT TTTCACACAC ATCTCCTGCT CTCAGAACCA GCAGTACTTG 
GTCAGGGCTT CATATTTGGA GATTTATCAG GAGGAGATCA GAGACCTTTT GTCAAAGGAC 
CAAGCACGTC GTTTAGAACT CAAAGAGCGA CCAGACACGG GCGTCTATGT TAAAGACCTG 
TCTTCGTTTG TCACTAAAAG TGTTCGTGAG ATTGAGCATG TGATGAATGT GGGGAACCAG 
AACCGCTCAG TTGGTGCAAC AAACATGAAT GAACACAGTT CTCGCTCTCA TGCCATCTTC 
GTCATCACCA TTGAGTGCAG TGAGTTGGGT CCTGATGGAG AGAACCATAT TCGAGTTGGA 
AAGCTTAACC TTGTTGACCT GGCAGGAAGT GAAAGACAGA CCAAAACCGG TGCTCAAGGT 
GAACGCTTAA AGGAAGCCAC TAAAATCAAT CTCTCCTTGT CAGCTTTGGG AAACGTCATC 
TCAGCATTGG TGGATGGAAG AAGCACCCAT ATCCCGTACC GTGACTCTAA GCTTACAAGA 
CTTCTTCAAG ACTCCTTGGG TGGAAACGCC CGAACGGTTA TGGTGGCCAA CATCGGACCA 
GCTTCCTACA ATGTAGAGGA GACTCTAACC ACGCTACGCT ATGCAAACCG CGCCAAAAAC 
ATCAAGAACA AACCCCGTGT CAATGAAGAC CCAAAGGATG CCCTTCTCCG GGAGTTTCAG 
GAGGAGATTG CCCGCTTGAA AGAACAGTTG GAAAAAAGGT CTGGAAGGAA GAGGAGGAGA 
AGGAGGAGAA GAAGGGTTGG AGAAGGAGGA GAGGAGTTTG AGGATGGGGA GGATGAAGAG 
GATGATGATG ATGATGATGA AGACGAAGAG GAAGGAGTGG ATGCAGATAA GAACATTGCA 
GATTATTGGC ATGAGCAACA GGAGAAGCTG GAGAAGGAGA GGAGAGCCAT CATGGAGGAC 
CACAGCCTGG TGGCAGAGGA GAAACAGCGG CTGCTGAAGG AGAAGGAGAG GAAGATGACT 
GACCTGCACA AAGAGAAAGA GGCATCAGAG ATGCTGACAG CCAAAGTTAA GGCCATGGAG 
AGCAAGCTGC TGGTAGGAGG AAAGAACATA GTGGACCACA CCAATGAGCA GCAGAAGGTT 
CTGGAGCTGA AGAGACAGGA GATCGCAGAA CAGAAAAGGC GGGAAAGGGA GATGAAGCAA 
GAGATGGAGT GTCGTGACGA GGAGACACTG GAGCTAAAAG AGACATACAG TTCACTGCAG 
CAGGAAGTTG ACATCAAGAC CAAGAAACTG AAAAAGCTCT TTTCCAAGCT TCAGTCTGTA 
AAGTCAGAGA TTCAGGATGC CCAGGATGAA CACGTGAAAT ACAGACAGGA ACTGGAACAG 
ACTCAGAATG AACTGACCAG AGAACTTAAA CTCAAACACT TAATCATAGA GAACTTCATC 
CCAATGGAGG AGAAGAACAA GATTGTAACA AGAGCCACAT TTGATGAAGA AGACGATCTC 
TGGAAAATGA CCCCCATCAC CCGTATACAA AACAGTGATC ATCAGATGAT GAAAAGGCCT 
GTTTCTGCAG TGGGCTACAG ACGGCCTCTG AGTCAGCATG CCCGTATGGC CATGTTGATG 
CGGCCTGATG TCAGATACAA GGCTGAGAAT ATCTTGCTGC TGGAGCTGGA TTTGCCCTCC 
CGGACCACAA AAGACTACGA GGGGCCAGTG ATCGCACCTA AAGTAGCTGC AGCTCTGGAG 
GACGCTCTGA GGGAGGAGGA CGAAATCCAG GTGGACGCCT CTGGTTTTCA TGCCAGCCTG 
GGCTCTAGTC CAGGCCTCAG TGCCTCAGCT GCTGGCTTTT CCAAGAAACC AAAGTCAGGC 
CGTCCAAAAA CAGGCAAGAA GGTGAGCACT CCAACTTCAG CCCACAGCCC TCTGTCCGGC 
TCAGGGTCTC CTCTTTACCC ACAATCCCGA GGCCTTGTGC CTAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001093615.1
CDS	239..2566
Translation	MSKSKSSESVKVVVRCRPMNEKERVANFNRVVSVDVKLGQVAVCNPRGASSHEHPKVFTFDSVYDWNSKQMELYDETFRPLVDSVLFGFNGTIFAYGQTGTGKTYTMEGVRNDPERRGVIPNSFEHIFTHISCSQNQQYLVRASYLEIYQEEIRDLLSKDQARRLELKERPDTGVYVKDLSSFVTKSVREIEHVMNVGNQNRSVGATNMNEHSSRSHAIFVITIECSELGPDGENHIRVGKLNLVDLAGSERQTKTGAQGERLKEATKINLSLSALGNVISALVDGRSTHIPYRDSKLTRLLQDSLGGNARTVMVANIGPASYNVEETLTTLRYANRAKNIKNKPRVNEDPKDALLREFQEEIARLKEQLEKRSGRKRRRRRRRRVGEGGEEFEDGEDEEDDDDDDEDEEEGVDADKNIADYWHEQQEKLEKERRAIMEDHSLVAEEKQRLLKEKERKMTDLHKEKEASEMLTAKVKAMESKLLVGGKNIVDHTNEQQKVLELKRQEIAEQKRREREMKQEMECRDEETLELKETYSSLQQEVDIKTKKLKKLFSKLQSVKSEIQDAQDEHVKYRQELEQTQNELTRELKLKHLIIENFIPMEEKNKIVTRATFDEEDDLWKMTPITRIQNSDHQMMKRPVSAVGYRRPLSQHARMAMLMRPDVRYKAENILLLELDLPSRTTKDYEGPVIAPKVAAALEDALREEDEIQVDASGFHASLGSSPGLSASAAGFSKKPKSGRPKTGKKVSTPTSAHSPLSGSGSPLYPQSRGLVPK

Target ORF information:

RefSeq Version	NM_001100145.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 3B (kif3b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001100145.1

ORF Insert Sequence:

ATGTCTAAAA GCAAGAGCTC TGAATCTGTT AAGGTTGTGG TGCGTTGTCG CCCCATGAAT 
GAGAAAGAGC GAGTCGCTAA CTTTAATAGA GTGGTATCTG TGGATGTGAA GTTAGGGCAA 
GTGGCAGTGT GCAACCCTCG AGGTGCATCT TCCCATGAGC ACCCAAAAGT GTTCACTTTT 
GATTCTGTCT ATGACTGGAA TTCAAAGCAG ATGGAGCTCT ACGACGAGAC TTTCCGCCCG 
TTAGTGGACT CTGTACTGTT TGGCTTCAAT GGGACCATCT TTGCATATGG CCAGACTGGC 
ACTGGAAAGA CTTACACAAT GGAAGGGGTA CGAAACGACC CGGAAAGAAG GGGTGTGATC 
CCAAACTCAT TTGAACACAT TTTCACACAC ATCTCCTGCT CTCAGAACCA GCAGTACTTG 
GTCAGGGCTT CATATTTGGA GATTTATCAG GAGGAGATCA GAGACCTTTT GTCAAAGGAC 
CAAGCACGTC GTTTAGAACT CAAAGAGCGA CCAGACACGG GCGTCTATGT TAAAGACCTG 
TCTTCGTTTG TCACTAAAAG TGTTCGTGAG ATTGAGCATG TGATGAATGT GGGGAACCAG 
AACCGCTCAG TTGGTGCAAC AAACATGAAT GAACACAGTT CTCGCTCTCA TGCCATCTTC 
GTCATCACCA TTGAGTGCAG TGAGTTGGGT CCTGATGGAG AGAACCATAT TCGAGTTGGA 
AAGCTTAACC TTGTTGACCT GGCAGGAAGT GAAAGACAGA CCAAAACCGG TGCTCAAGGT 
GAACGCTTAA AGGAAGCCAC TAAAATCAAT CTCTCCTTGT CAGCTTTGGG AAACGTCATC 
TCAGCATTGG TGGATGGAAG AAGCACCCAT ATCCCGTACC GTGACTCTAA GCTTACAAGA 
CTTCTTCAAG ACTCCTTGGG TGGAAACGCC CGAACGGTTA TGGTGGCCAA CATCGGACCA 
GCTTCCTACA ATGTAGAGGA GACTCTAACC ACGCTACGCT ATGCAAACCG CGCCAAAAAC 
ATCAAGAACA AACCCCGTGT CAATGAAGAC CCAAAGGATG CCCTTCTCCG GGAGTTTCAG 
GAGGAGATTG CCCGCTTGAA AGAACAGTTG GAAAAAAGGT CTGGAAGGAA GAGGAGGAGA 
AGGAGGAGAA GAAGGGTTGG AGAAGGAGGA GAGGAGTTTG AGGATGGGGA GGATGAAGAG 
GATGATGATG ATGATGATGA AGACGAAGAG GAAGGAGTGG ATGCAGATAA GAACATTGCA 
GATTATTGGC ATGAGCAACA GGAGAAGCTG GAGAAGGAGA GGAGAGCCAT CATGGAGGAC 
CACAGCCTGG TGGCAGAGGA GAAACAGCGG CTGCTGAAGG AGAAGGAGAG GAAGATGACT 
GACCTGCACA AAGAGAAAGA GGCATCAGAG ATGCTGACAG CCAAAGTTAA GGCCATGGAG 
AGCAAGCTGC TGGTAGGAGG AAAGAACATA GTGGACCACA CCAATGAGCA GCAGAAGGTT 
CTGGAGCTGA AGAGACAGGA GATCGCAGAA CAGAAAAGGC GGGAAAGGGA GATGAAGCAA 
GAGATGGAGT GTCGTGACGA GGAGACACTG GAGCTAAAAG AGACATACAG TTCACTGCAG 
CAGGAAGTTG ACATCAAGAC CAAGAAACTG AAAAAGCTCT TTTCCAAGCT TCAGTCTGTA 
AAGTCAGAGA TTCAGGATGC CCAGGATGAA CACGTGAAAT ACAGACAGGA ACTGGAACAG 
ACTCAGAATG AACTGACCAG AGAACTTAAA CTCAAACACT TAATCATAGA GAACTTCATC 
CCAATGGAGG AGAAGAACAA GATTGTAACA AGAGCCACAT TTGATGAAGA AGACGATCTC 
TGGAAAATGA CCCCCATCAC CCGTATACAA AACAGTGATC ATCAGATGAT GAAAAGGCCT 
GTTTCTGCAG TGGGCTACAG ACGGCCTCTG AGTCAGCATG CCCGTATGGC CATGTTGATG 
CGGCCTGATG TCAGATACAA GGCTGAGAAT ATCTTGCTGC TGGAGCTGGA TTTGCCCTCC 
CGGACCACAA AAGACTACGA GGGGCCAGTG ATCGCACCTA AAGTAGCTGC AGCTCTGGAG 
GACGCTCTGA GGGAGGAGGA CGAAATCCAG GTGGACGCCT CTGGTTTTCA TGCCAGCCTG 
GGCTCTAGTC CAGGCCTCAG TGCCTCAGCT GCTGGCTTTT CCAAGAAACC AAAGTCAGGC 
CGTCCAAAAA CAGGCAAGAA GGTGAGCACT CCAACTTCAG CCCACAGCCC TCTGTCCGGC 
TCAGGGTCTC CTCTTTACCC ACAATCCCGA GGCCTTGTGC CTAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF3B ( XM_009296629.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009294904.1 Danio rerio kinesin family member 3B (kif3b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa27402
Clone ID Related Accession (Same CDS sequence)	XM_009296629.2
Accession Version	XM_009296629.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	kinesin-like protein KIF3B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296629.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAAAA GCAAGAGCTC TGAATCTGTT AAGGTGGTGG TGCGTTGTCG CCCCATGAAT 
GAGAAAGAGC GAGTCGCTAA CTTCAATAGA GTGGTATCTG TGGATGTGAA GTTAGGGCAA 
GTGGCAGTGT GCAACCCTCG AGGTGCATCT TCCCATGAGC ACCCAAAAGT GTTCACTTTT 
GATTCTGTCT ATGACTGGAA TTCAAAGCAG ATGGAGCTCT ACGACGAGAC TTTCCGCCCG 
TTAGTGGACT CTGTACTGTT TGGCTTCAAT GGGACCATCT TTGCATATGG CCAGACTGGC 
ACTGGAAAGA CTTACACAAT GGAAGGGGTA CGAAACGACC CGGAAAGAAG GGGTGTGATC 
CCAAACTCAT TTGAACACAT TTTCACACAC ATCTCCCGCT CTCAGAACCA GCAGTACTTG 
GTCAGGGCTT CATATTTGGA GATTTATCAG GAGGAGATCA GAGACCTTTT GTCAAAGGAC 
CAAGCACGTC GTTTGGAACT CAAAGAGCGA CCAGACACAG GTGTCTATGT TAAAGACCTG 
TCTTCGTTTG TCACTAAAAG TGTTCGTGAG ATTGAGCATG TGATGAATGT GGGGAACCAG 
AACCGCTCAG TTGGTGCAAC AAACATGAAT GAACACAGTT CTCGCTCTCA TGCCATCTTC 
GTCATCACCA TTGAGTGCAG TGAGTTGGGT CCTGACGGAG AGAACCATAT TCGAGTTGGG 
AAGCTTAACC TTGTTGACCT GGCAGGAAGT GAAAGACAGA CCAAAACCGG TGCTCAAGGT 
GAACGCTTAA AAGAAGCCAC TAAAATCAAT CTCTCCTTGT CAGCTTTGGG AAACGTCATC 
TCAGCGTTGG TGGATGGAAG AAGCACCCAT ATCCCGTACC GTGACTCTAA GCTTACAAGA 
CTTCTTCAAG ACTCCTTGGG TGGAAACGCC CGAACGGTTA TGGTGGCCAA CATTGGACCA 
GCTTCCTACA ATGTAGAGGA GACTCTAACG ACGCTACGCT ATGCAAACCG CGCCAAAAAC 
ATCAAGAACA AACCCCGTGT CAATGAAGAC CCCAAGGATG CCCTTCTTCG GGAGTTTCAG 
GAGGAGATTG CCCGCTTGAA AGAACAGTTG GAGAAAAGGT CTGGAAGGAA GAGGAGGAGA 
AGGAGGAGAA GAAGGGTTGG AGAAGGAGGA GAGGAGTTTG AGGATGGGGA GGATGAAGAG 
GATGATGATG ATGATGATGA AGACGAAGAG GAAGGAGTGG ATGCAGATAA GAACATTGCA 
GATTATTGGC ATGAGCAACA GGAGAAGCTG GAGAAGGAGA GGAGAGCCAT CATGGAGGAC 
CACAGTCTGG TGGCAGAGGA GAAACAGCGG CTGCTGAAGG AGAAGGAGAG GAAGATGACT 
GACCTGCACA AAGAGAAAGA GGCATCAGAG ATGCTGACAG CCAAAGTTAA GGCCATGGAG 
AGCAAGCTGC TGGTAGGAGG AAAGAACATA GTGGACCACA CCAACGAGCA GCAGAAGGTT 
CTGGAGCTGA AGAGACAGGA GATCGCAGAA CAGAAAAGGC GGGAAAGGGA GATGAAGCAA 
GAGATGGAGT GTCGTGACGA GGAGACACTG GAGCTAAAAG AGACGTACAG TTCACTGCAG 
CAGGAAGTTG ACATCAAGAC CAAGAAACTG AAAAAGCTCT TTTCCAAGCT TCAGTCTGTA 
AAGTCAGAGA TTCAGGATGC CCAGGATGAG CACGTGAAAT ACAGACAGGA ACTGGAACAG 
ACTCAGAATG AACTGACCAG AGAACTTAAA CTCAAACACT TAATCATAGA GAACTTCATC 
CCAATGGAGG AGAAGAACAA GATTGTAACA AGAGCCACAT TTGATGAAGA AGACGATCTC 
TGGAAAATGA CCCCCATCAC CCGTATACAA AACAGTGATC ATCAGATGAT GAAAAGGCCT 
GTTTCTGCAG TGGGCTACAG ACGGCCTCTG AGTCAGCATG CCCGTATGGC CATGTTGATG 
CGGCCTGATG TCAGATACAA GGCTGAGAAT ATCTTGCTGC TGGAGCTGGA TTTGCCCTCC 
CGGACCACAA AAGACTACGA GGGGCCAGTG ATCGCACCTA AAGTAGCTGC AGCTCTGGAG 
GACGCTCTGA GGGAGGAGGA CGAAATCCAG GTGGACGCCT CTGGTTTTCA TGCCAGCCTG 
GGCTCTAGTC CAGGCCTCAG TGCCTCAGCT GCTGGCTTTT CCAAGAAACC AAAGTCAGGC 
CGTCCAAAAA CAGGCAAGAA GGTGAGCACT CCAACTTCAG CCCATAGCCC TCTGTCCGGC 
TCAGGGTCTC CTCTTTACCC ACAATCCCGA GGCCTTGTGC CTAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294904.1
CDS	112..2439
Translation	MSKSKSSESVKVVVRCRPMNEKERVANFNRVVSVDVKLGQVAVCNPRGASSHEHPKVFTFDSVYDWNSKQMELYDETFRPLVDSVLFGFNGTIFAYGQTGTGKTYTMEGVRNDPERRGVIPNSFEHIFTHISRSQNQQYLVRASYLEIYQEEIRDLLSKDQARRLELKERPDTGVYVKDLSSFVTKSVREIEHVMNVGNQNRSVGATNMNEHSSRSHAIFVITIECSELGPDGENHIRVGKLNLVDLAGSERQTKTGAQGERLKEATKINLSLSALGNVISALVDGRSTHIPYRDSKLTRLLQDSLGGNARTVMVANIGPASYNVEETLTTLRYANRAKNIKNKPRVNEDPKDALLREFQEEIARLKEQLEKRSGRKRRRRRRRRVGEGGEEFEDGEDEEDDDDDDEDEEEGVDADKNIADYWHEQQEKLEKERRAIMEDHSLVAEEKQRLLKEKERKMTDLHKEKEASEMLTAKVKAMESKLLVGGKNIVDHTNEQQKVLELKRQEIAEQKRREREMKQEMECRDEETLELKETYSSLQQEVDIKTKKLKKLFSKLQSVKSEIQDAQDEHVKYRQELEQTQNELTRELKLKHLIIENFIPMEEKNKIVTRATFDEEDDLWKMTPITRIQNSDHQMMKRPVSAVGYRRPLSQHARMAMLMRPDVRYKAENILLLELDLPSRTTKDYEGPVIAPKVAAALEDALREEDEIQVDASGFHASLGSSPGLSASAAGFSKKPKSGRPKTGKKVSTPTSAHSPLSGSGSPLYPQSRGLVPK

Target ORF information:

RefSeq Version	XM_009296629.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio kinesin family member 3B (kif3b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296629.2

ORF Insert Sequence:

ATGTCTAAAA GCAAGAGCTC TGAATCTGTT AAGGTGGTGG TGCGTTGTCG CCCCATGAAT 
GAGAAAGAGC GAGTCGCTAA CTTCAATAGA GTGGTATCTG TGGATGTGAA GTTAGGGCAA 
GTGGCAGTGT GCAACCCTCG AGGTGCATCT TCCCATGAGC ACCCAAAAGT GTTCACTTTT 
GATTCTGTCT ATGACTGGAA TTCAAAGCAG ATGGAGCTCT ACGACGAGAC TTTCCGCCCG 
TTAGTGGACT CTGTACTGTT TGGCTTCAAT GGGACCATCT TTGCATATGG CCAGACTGGC 
ACTGGAAAGA CTTACACAAT GGAAGGGGTA CGAAACGACC CGGAAAGAAG GGGTGTGATC 
CCAAACTCAT TTGAACACAT TTTCACACAC ATCTCCCGCT CTCAGAACCA GCAGTACTTG 
GTCAGGGCTT CATATTTGGA GATTTATCAG GAGGAGATCA GAGACCTTTT GTCAAAGGAC 
CAAGCACGTC GTTTGGAACT CAAAGAGCGA CCAGACACAG GTGTCTATGT TAAAGACCTG 
TCTTCGTTTG TCACTAAAAG TGTTCGTGAG ATTGAGCATG TGATGAATGT GGGGAACCAG 
AACCGCTCAG TTGGTGCAAC AAACATGAAT GAACACAGTT CTCGCTCTCA TGCCATCTTC 
GTCATCACCA TTGAGTGCAG TGAGTTGGGT CCTGACGGAG AGAACCATAT TCGAGTTGGG 
AAGCTTAACC TTGTTGACCT GGCAGGAAGT GAAAGACAGA CCAAAACCGG TGCTCAAGGT 
GAACGCTTAA AAGAAGCCAC TAAAATCAAT CTCTCCTTGT CAGCTTTGGG AAACGTCATC 
TCAGCGTTGG TGGATGGAAG AAGCACCCAT ATCCCGTACC GTGACTCTAA GCTTACAAGA 
CTTCTTCAAG ACTCCTTGGG TGGAAACGCC CGAACGGTTA TGGTGGCCAA CATTGGACCA 
GCTTCCTACA ATGTAGAGGA GACTCTAACG ACGCTACGCT ATGCAAACCG CGCCAAAAAC 
ATCAAGAACA AACCCCGTGT CAATGAAGAC CCCAAGGATG CCCTTCTTCG GGAGTTTCAG 
GAGGAGATTG CCCGCTTGAA AGAACAGTTG GAGAAAAGGT CTGGAAGGAA GAGGAGGAGA 
AGGAGGAGAA GAAGGGTTGG AGAAGGAGGA GAGGAGTTTG AGGATGGGGA GGATGAAGAG 
GATGATGATG ATGATGATGA AGACGAAGAG GAAGGAGTGG ATGCAGATAA GAACATTGCA 
GATTATTGGC ATGAGCAACA GGAGAAGCTG GAGAAGGAGA GGAGAGCCAT CATGGAGGAC 
CACAGTCTGG TGGCAGAGGA GAAACAGCGG CTGCTGAAGG AGAAGGAGAG GAAGATGACT 
GACCTGCACA AAGAGAAAGA GGCATCAGAG ATGCTGACAG CCAAAGTTAA GGCCATGGAG 
AGCAAGCTGC TGGTAGGAGG AAAGAACATA GTGGACCACA CCAACGAGCA GCAGAAGGTT 
CTGGAGCTGA AGAGACAGGA GATCGCAGAA CAGAAAAGGC GGGAAAGGGA GATGAAGCAA 
GAGATGGAGT GTCGTGACGA GGAGACACTG GAGCTAAAAG AGACGTACAG TTCACTGCAG 
CAGGAAGTTG ACATCAAGAC CAAGAAACTG AAAAAGCTCT TTTCCAAGCT TCAGTCTGTA 
AAGTCAGAGA TTCAGGATGC CCAGGATGAG CACGTGAAAT ACAGACAGGA ACTGGAACAG 
ACTCAGAATG AACTGACCAG AGAACTTAAA CTCAAACACT TAATCATAGA GAACTTCATC 
CCAATGGAGG AGAAGAACAA GATTGTAACA AGAGCCACAT TTGATGAAGA AGACGATCTC 
TGGAAAATGA CCCCCATCAC CCGTATACAA AACAGTGATC ATCAGATGAT GAAAAGGCCT 
GTTTCTGCAG TGGGCTACAG ACGGCCTCTG AGTCAGCATG CCCGTATGGC CATGTTGATG 
CGGCCTGATG TCAGATACAA GGCTGAGAAT ATCTTGCTGC TGGAGCTGGA TTTGCCCTCC 
CGGACCACAA AAGACTACGA GGGGCCAGTG ATCGCACCTA AAGTAGCTGC AGCTCTGGAG 
GACGCTCTGA GGGAGGAGGA CGAAATCCAG GTGGACGCCT CTGGTTTTCA TGCCAGCCTG 
GGCTCTAGTC CAGGCCTCAG TGCCTCAGCT GCTGGCTTTT CCAAGAAACC AAAGTCAGGC 
CGTCCAAAAA CAGGCAAGAA GGTGAGCACT CCAACTTCAG CCCATAGCCC TCTGTCCGGC 
TCAGGGTCTC CTCTTTACCC ACAATCCCGA GGCCTTGTGC CTAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF3B ( XM_005174101.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005174158.1 Danio rerio kinesin family member 3B (kif3b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa27403
Clone ID Related Accession (Same CDS sequence)	XM_005174101.4 , XM_005174101.3
Accession Version	XM_005174101.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kinesin-like protein KIF3B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005174101.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAAAA GCAAGAGCTC TGAATCTGTT AAGGTGGTGG TGCGTTGTCG CCCCATGAAT 
GAGAAAGAGC GAGTCGCTAA CTTCAATAGA GTGGTATCTG TGGATGTGAA GTTAGGGCAA 
GTGGCAGTGT GCAACCCTCG AGGTGCATCT TCCCATGAGC ACCCAAAAGT GTTCACTTTT 
GATTCTGTCT ATGACTGGAA TTCAAAGCAG ATGGAGCTCT ACGACGAGAC TTTCCGCCCG 
TTAGTGGACT CTGTACTGTT TGGCTTCAAT GGGACCATCT TTGCATATGG CCAGACTGGC 
ACTGGAAAGA CTTACACAAT GGAAGGGGTA CGAAACGACC CGGAAAGAAG GGGTGTGATC 
CCAAACTCAT TTGAACACAT TTTCACACAC ATCTCCCGCT CTCAGAACCA GCAGTACTTG 
GTCAGGGCTT CATATTTGGA GATTTATCAG GAGGAGATCA GAGACCTTTT GTCAAAGGAC 
CAAGCACGTC GTTTGGAACT CAAAGAGCGA CCAGACACAG GTGTCTATGT TAAAGACCTG 
TCTTCGTTTG TCACTAAAAG TGTTCGTGAG ATTGAGCATG TGATGAATGT GGGGAACCAG 
AACCGCTCAG TTGGTGCAAC AAACATGAAT GAACACAGTT CTCGCTCTCA TGCCATCTTC 
GTCATCACCA TTGAGTGCAG TGAGTTGGGT CCTGACGGAG AGAACCATAT TCGAGTTGGG 
AAGCTTAACC TTGTTGACCT GGCAGGAAGT GAAAGACAGA CCAAAACCGG TGCTCAAGGT 
GAACGCTTAA AAGAAGCCAC TAAAATCAAT CTCTCCTTGT CAGCTTTGGG AAACGTCATC 
TCAGCGTTGG TGGATGGAAG AAGCACCCAT ATCCCGTACC GTGACTCTAA GCTTACAAGA 
CTTCTTCAAG ACTCCTTGGG TGGAAACGCC CGAACGGTTA TGGTGGCCAA CATTGGACCA 
GCTTCCTACA ATGTAGAGGA GACTCTAACG ACGCTACGCT ATGCAAACCG CGCCAAAAAC 
ATCAAGAACA AACCCCGTGT CAATGAAGAC CCCAAGGATG CCCTTCTTCG GGAGTTTCAG 
GAGGAGATTG CCCGCTTGAA AGAACAGTTG GAGAAAAGGT CTGGAAGGAA GAGGAGGAGA 
AGGAGGAGAA GAAGGGTTGG AGAAGGAGGA GAGGAGTTTG AGGATGGGGA GGATGAAGAG 
GATGATGATG ATGATGATGA AGACGAAGAG GAAGGAGTGG ATGCAGATAA GAACATTGCA 
GATTATTGGC ATGAGCAACA GGAGAAGCTG GAGAAGGAGA GGAGAGCCAT CATGGAGGAC 
CACAGTCTGG TGGCAGAGGA GAAACAGCGG CTGCTGAAGG AGAAGGAGAG GAAGATGACT 
GACCTGCACA AAGAGAAAGA GGCATCAGAG ATGCTGACAG CCAAAGTTAA GGCCATGGAG 
AGCAAGCTGC TGGTAGGAGG AAAGAACATA GTGGACCACA CCAACGAGCA GCAGAAGGTT 
CTGGAGCTGA AGAGACAGGA GATCGCAGAA CAGAAAAGGC GGGAAAGGGA GATGAAGCAA 
GAGATGGAGT GTCGTGACGA GGAGACACTG GAGCTAAAAG AGACGTACAG TTCACTGCAG 
CAGGAAGTTG ACATCAAGAC CAAGAAACTG AAAAAGCTCT TTTCCAAGCT TCAGTCTGTA 
AAGTCAGAGA TTCAGGATGC CCAGGATGAG CACGTGAAAT ACAGACAGGA ACTGGAACAG 
ACTCAGAATG AACTGACCAG AGAACTTAAA CTCAAACACT TAATCATAGA GAACTTCATC 
CCAATGGAGG AGAAGAACAA GATTGTAACA AGAGCCACAT TTGATGAAGA AGACGATCTC 
TGGAAAATGA CCCCCATCAC CCGTATACAA AATGATCATC AGATGATGAA AAGGCCTGTT 
TCTGCAGTGG GCTACAGACG GCCTCTGAGT CAGCATGCCC GTATGGCCAT GTTGATGCGG 
CCTGATGTCA GATACAAGGC TGAGAATATC TTGCTGCTGG AGCTGGATTT GCCCTCCCGG 
ACCACAAAAG ACTACGAGGG GCCAGTGATC GCACCTAAAG TAGCTGCAGC TCTGGAGGAC 
GCTCTGAGGG AGGAGGACGA AATCCAGGTG GACGCCTCTG GTTTTCATGC CAGCCTGGGC 
TCTAGTCCAG GCCTCAGTGC CTCAGCTGCT GGCTTTTCCA AGAAACCAAA GTCAGGCCGT 
CCAAAAACAG GCAAGAAGGT GAGCACTCCA ACTTCAGCCC ATAGCCCTCT GTCCGGCTCA 
GGGTCTCCTC TTTACCCACA ATCCCGAGGC CTTGTGCCTA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005174158.1
CDS	323..2647
Translation	MSKSKSSESVKVVVRCRPMNEKERVANFNRVVSVDVKLGQVAVCNPRGASSHEHPKVFTFDSVYDWNSKQMELYDETFRPLVDSVLFGFNGTIFAYGQTGTGKTYTMEGVRNDPERRGVIPNSFEHIFTHISRSQNQQYLVRASYLEIYQEEIRDLLSKDQARRLELKERPDTGVYVKDLSSFVTKSVREIEHVMNVGNQNRSVGATNMNEHSSRSHAIFVITIECSELGPDGENHIRVGKLNLVDLAGSERQTKTGAQGERLKEATKINLSLSALGNVISALVDGRSTHIPYRDSKLTRLLQDSLGGNARTVMVANIGPASYNVEETLTTLRYANRAKNIKNKPRVNEDPKDALLREFQEEIARLKEQLEKRSGRKRRRRRRRRVGEGGEEFEDGEDEEDDDDDDEDEEEGVDADKNIADYWHEQQEKLEKERRAIMEDHSLVAEEKQRLLKEKERKMTDLHKEKEASEMLTAKVKAMESKLLVGGKNIVDHTNEQQKVLELKRQEIAEQKRREREMKQEMECRDEETLELKETYSSLQQEVDIKTKKLKKLFSKLQSVKSEIQDAQDEHVKYRQELEQTQNELTRELKLKHLIIENFIPMEEKNKIVTRATFDEEDDLWKMTPITRIQNDHQMMKRPVSAVGYRRPLSQHARMAMLMRPDVRYKAENILLLELDLPSRTTKDYEGPVIAPKVAAALEDALREEDEIQVDASGFHASLGSSPGLSASAAGFSKKPKSGRPKTGKKVSTPTSAHSPLSGSGSPLYPQSRGLVPK

Target ORF information:

RefSeq Version	XM_005174101.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kinesin family member 3B (kif3b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005174101.4

ORF Insert Sequence:

ATGTCTAAAA GCAAGAGCTC TGAATCTGTT AAGGTGGTGG TGCGTTGTCG CCCCATGAAT 
GAGAAAGAGC GAGTCGCTAA CTTCAATAGA GTGGTATCTG TGGATGTGAA GTTAGGGCAA 
GTGGCAGTGT GCAACCCTCG AGGTGCATCT TCCCATGAGC ACCCAAAAGT GTTCACTTTT 
GATTCTGTCT ATGACTGGAA TTCAAAGCAG ATGGAGCTCT ACGACGAGAC TTTCCGCCCG 
TTAGTGGACT CTGTACTGTT TGGCTTCAAT GGGACCATCT TTGCATATGG CCAGACTGGC 
ACTGGAAAGA CTTACACAAT GGAAGGGGTA CGAAACGACC CGGAAAGAAG GGGTGTGATC 
CCAAACTCAT TTGAACACAT TTTCACACAC ATCTCCCGCT CTCAGAACCA GCAGTACTTG 
GTCAGGGCTT CATATTTGGA GATTTATCAG GAGGAGATCA GAGACCTTTT GTCAAAGGAC 
CAAGCACGTC GTTTGGAACT CAAAGAGCGA CCAGACACAG GTGTCTATGT TAAAGACCTG 
TCTTCGTTTG TCACTAAAAG TGTTCGTGAG ATTGAGCATG TGATGAATGT GGGGAACCAG 
AACCGCTCAG TTGGTGCAAC AAACATGAAT GAACACAGTT CTCGCTCTCA TGCCATCTTC 
GTCATCACCA TTGAGTGCAG TGAGTTGGGT CCTGACGGAG AGAACCATAT TCGAGTTGGG 
AAGCTTAACC TTGTTGACCT GGCAGGAAGT GAAAGACAGA CCAAAACCGG TGCTCAAGGT 
GAACGCTTAA AAGAAGCCAC TAAAATCAAT CTCTCCTTGT CAGCTTTGGG AAACGTCATC 
TCAGCGTTGG TGGATGGAAG AAGCACCCAT ATCCCGTACC GTGACTCTAA GCTTACAAGA 
CTTCTTCAAG ACTCCTTGGG TGGAAACGCC CGAACGGTTA TGGTGGCCAA CATTGGACCA 
GCTTCCTACA ATGTAGAGGA GACTCTAACG ACGCTACGCT ATGCAAACCG CGCCAAAAAC 
ATCAAGAACA AACCCCGTGT CAATGAAGAC CCCAAGGATG CCCTTCTTCG GGAGTTTCAG 
GAGGAGATTG CCCGCTTGAA AGAACAGTTG GAGAAAAGGT CTGGAAGGAA GAGGAGGAGA 
AGGAGGAGAA GAAGGGTTGG AGAAGGAGGA GAGGAGTTTG AGGATGGGGA GGATGAAGAG 
GATGATGATG ATGATGATGA AGACGAAGAG GAAGGAGTGG ATGCAGATAA GAACATTGCA 
GATTATTGGC ATGAGCAACA GGAGAAGCTG GAGAAGGAGA GGAGAGCCAT CATGGAGGAC 
CACAGTCTGG TGGCAGAGGA GAAACAGCGG CTGCTGAAGG AGAAGGAGAG GAAGATGACT 
GACCTGCACA AAGAGAAAGA GGCATCAGAG ATGCTGACAG CCAAAGTTAA GGCCATGGAG 
AGCAAGCTGC TGGTAGGAGG AAAGAACATA GTGGACCACA CCAACGAGCA GCAGAAGGTT 
CTGGAGCTGA AGAGACAGGA GATCGCAGAA CAGAAAAGGC GGGAAAGGGA GATGAAGCAA 
GAGATGGAGT GTCGTGACGA GGAGACACTG GAGCTAAAAG AGACGTACAG TTCACTGCAG 
CAGGAAGTTG ACATCAAGAC CAAGAAACTG AAAAAGCTCT TTTCCAAGCT TCAGTCTGTA 
AAGTCAGAGA TTCAGGATGC CCAGGATGAG CACGTGAAAT ACAGACAGGA ACTGGAACAG 
ACTCAGAATG AACTGACCAG AGAACTTAAA CTCAAACACT TAATCATAGA GAACTTCATC 
CCAATGGAGG AGAAGAACAA GATTGTAACA AGAGCCACAT TTGATGAAGA AGACGATCTC 
TGGAAAATGA CCCCCATCAC CCGTATACAA AATGATCATC AGATGATGAA AAGGCCTGTT 
TCTGCAGTGG GCTACAGACG GCCTCTGAGT CAGCATGCCC GTATGGCCAT GTTGATGCGG 
CCTGATGTCA GATACAAGGC TGAGAATATC TTGCTGCTGG AGCTGGATTT GCCCTCCCGG 
ACCACAAAAG ACTACGAGGG GCCAGTGATC GCACCTAAAG TAGCTGCAGC TCTGGAGGAC 
GCTCTGAGGG AGGAGGACGA AATCCAGGTG GACGCCTCTG GTTTTCATGC CAGCCTGGGC 
TCTAGTCCAG GCCTCAGTGC CTCAGCTGCT GGCTTTTCCA AGAAACCAAA GTCAGGCCGT 
CCAAAAACAG GCAAGAAGGT GAGCACTCCA ACTTCAGCCC ATAGCCCTCT GTCCGGCTCA 
GGGTCTCCTC TTTACCCACA ATCCCGAGGC CTTGTGCCTA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF3B ( XM_005174101.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005174158.1 Danio rerio kinesin family member 3B (kif3b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa27403
Clone ID Related Accession (Same CDS sequence)	XM_005174101.4 , XM_005174101.3
Accession Version	XM_005174101.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	kinesin-like protein KIF3B isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005174101.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAAAA GCAAGAGCTC TGAATCTGTT AAGGTGGTGG TGCGTTGTCG CCCCATGAAT 
GAGAAAGAGC GAGTCGCTAA CTTCAATAGA GTGGTATCTG TGGATGTGAA GTTAGGGCAA 
GTGGCAGTGT GCAACCCTCG AGGTGCATCT TCCCATGAGC ACCCAAAAGT GTTCACTTTT 
GATTCTGTCT ATGACTGGAA TTCAAAGCAG ATGGAGCTCT ACGACGAGAC TTTCCGCCCG 
TTAGTGGACT CTGTACTGTT TGGCTTCAAT GGGACCATCT TTGCATATGG CCAGACTGGC 
ACTGGAAAGA CTTACACAAT GGAAGGGGTA CGAAACGACC CGGAAAGAAG GGGTGTGATC 
CCAAACTCAT TTGAACACAT TTTCACACAC ATCTCCCGCT CTCAGAACCA GCAGTACTTG 
GTCAGGGCTT CATATTTGGA GATTTATCAG GAGGAGATCA GAGACCTTTT GTCAAAGGAC 
CAAGCACGTC GTTTGGAACT CAAAGAGCGA CCAGACACAG GTGTCTATGT TAAAGACCTG 
TCTTCGTTTG TCACTAAAAG TGTTCGTGAG ATTGAGCATG TGATGAATGT GGGGAACCAG 
AACCGCTCAG TTGGTGCAAC AAACATGAAT GAACACAGTT CTCGCTCTCA TGCCATCTTC 
GTCATCACCA TTGAGTGCAG TGAGTTGGGT CCTGACGGAG AGAACCATAT TCGAGTTGGG 
AAGCTTAACC TTGTTGACCT GGCAGGAAGT GAAAGACAGA CCAAAACCGG TGCTCAAGGT 
GAACGCTTAA AAGAAGCCAC TAAAATCAAT CTCTCCTTGT CAGCTTTGGG AAACGTCATC 
TCAGCGTTGG TGGATGGAAG AAGCACCCAT ATCCCGTACC GTGACTCTAA GCTTACAAGA 
CTTCTTCAAG ACTCCTTGGG TGGAAACGCC CGAACGGTTA TGGTGGCCAA CATTGGACCA 
GCTTCCTACA ATGTAGAGGA GACTCTAACG ACGCTACGCT ATGCAAACCG CGCCAAAAAC 
ATCAAGAACA AACCCCGTGT CAATGAAGAC CCCAAGGATG CCCTTCTTCG GGAGTTTCAG 
GAGGAGATTG CCCGCTTGAA AGAACAGTTG GAGAAAAGGT CTGGAAGGAA GAGGAGGAGA 
AGGAGGAGAA GAAGGGTTGG AGAAGGAGGA GAGGAGTTTG AGGATGGGGA GGATGAAGAG 
GATGATGATG ATGATGATGA AGACGAAGAG GAAGGAGTGG ATGCAGATAA GAACATTGCA 
GATTATTGGC ATGAGCAACA GGAGAAGCTG GAGAAGGAGA GGAGAGCCAT CATGGAGGAC 
CACAGTCTGG TGGCAGAGGA GAAACAGCGG CTGCTGAAGG AGAAGGAGAG GAAGATGACT 
GACCTGCACA AAGAGAAAGA GGCATCAGAG ATGCTGACAG CCAAAGTTAA GGCCATGGAG 
AGCAAGCTGC TGGTAGGAGG AAAGAACATA GTGGACCACA CCAACGAGCA GCAGAAGGTT 
CTGGAGCTGA AGAGACAGGA GATCGCAGAA CAGAAAAGGC GGGAAAGGGA GATGAAGCAA 
GAGATGGAGT GTCGTGACGA GGAGACACTG GAGCTAAAAG AGACGTACAG TTCACTGCAG 
CAGGAAGTTG ACATCAAGAC CAAGAAACTG AAAAAGCTCT TTTCCAAGCT TCAGTCTGTA 
AAGTCAGAGA TTCAGGATGC CCAGGATGAG CACGTGAAAT ACAGACAGGA ACTGGAACAG 
ACTCAGAATG AACTGACCAG AGAACTTAAA CTCAAACACT TAATCATAGA GAACTTCATC 
CCAATGGAGG AGAAGAACAA GATTGTAACA AGAGCCACAT TTGATGAAGA AGACGATCTC 
TGGAAAATGA CCCCCATCAC CCGTATACAA AATGATCATC AGATGATGAA AAGGCCTGTT 
TCTGCAGTGG GCTACAGACG GCCTCTGAGT CAGCATGCCC GTATGGCCAT GTTGATGCGG 
CCTGATGTCA GATACAAGGC TGAGAATATC TTGCTGCTGG AGCTGGATTT GCCCTCCCGG 
ACCACAAAAG ACTACGAGGG GCCAGTGATC GCACCTAAAG TAGCTGCAGC TCTGGAGGAC 
GCTCTGAGGG AGGAGGACGA AATCCAGGTG GACGCCTCTG GTTTTCATGC CAGCCTGGGC 
TCTAGTCCAG GCCTCAGTGC CTCAGCTGCT GGCTTTTCCA AGAAACCAAA GTCAGGCCGT 
CCAAAAACAG GCAAGAAGGT GAGCACTCCA ACTTCAGCCC ATAGCCCTCT GTCCGGCTCA 
GGGTCTCCTC TTTACCCACA ATCCCGAGGC CTTGTGCCTA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005174158.1
CDS	328..2652
Translation	MSKSKSSESVKVVVRCRPMNEKERVANFNRVVSVDVKLGQVAVCNPRGASSHEHPKVFTFDSVYDWNSKQMELYDETFRPLVDSVLFGFNGTIFAYGQTGTGKTYTMEGVRNDPERRGVIPNSFEHIFTHISRSQNQQYLVRASYLEIYQEEIRDLLSKDQARRLELKERPDTGVYVKDLSSFVTKSVREIEHVMNVGNQNRSVGATNMNEHSSRSHAIFVITIECSELGPDGENHIRVGKLNLVDLAGSERQTKTGAQGERLKEATKINLSLSALGNVISALVDGRSTHIPYRDSKLTRLLQDSLGGNARTVMVANIGPASYNVEETLTTLRYANRAKNIKNKPRVNEDPKDALLREFQEEIARLKEQLEKRSGRKRRRRRRRRVGEGGEEFEDGEDEEDDDDDDEDEEEGVDADKNIADYWHEQQEKLEKERRAIMEDHSLVAEEKQRLLKEKERKMTDLHKEKEASEMLTAKVKAMESKLLVGGKNIVDHTNEQQKVLELKRQEIAEQKRREREMKQEMECRDEETLELKETYSSLQQEVDIKTKKLKKLFSKLQSVKSEIQDAQDEHVKYRQELEQTQNELTRELKLKHLIIENFIPMEEKNKIVTRATFDEEDDLWKMTPITRIQNDHQMMKRPVSAVGYRRPLSQHARMAMLMRPDVRYKAENILLLELDLPSRTTKDYEGPVIAPKVAAALEDALREEDEIQVDASGFHASLGSSPGLSASAAGFSKKPKSGRPKTGKKVSTPTSAHSPLSGSGSPLYPQSRGLVPK

Target ORF information:

RefSeq Version	XM_005174101.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio kinesin family member 3B (kif3b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005174101.3

ORF Insert Sequence:

ATGTCTAAAA GCAAGAGCTC TGAATCTGTT AAGGTGGTGG TGCGTTGTCG CCCCATGAAT 
GAGAAAGAGC GAGTCGCTAA CTTCAATAGA GTGGTATCTG TGGATGTGAA GTTAGGGCAA 
GTGGCAGTGT GCAACCCTCG AGGTGCATCT TCCCATGAGC ACCCAAAAGT GTTCACTTTT 
GATTCTGTCT ATGACTGGAA TTCAAAGCAG ATGGAGCTCT ACGACGAGAC TTTCCGCCCG 
TTAGTGGACT CTGTACTGTT TGGCTTCAAT GGGACCATCT TTGCATATGG CCAGACTGGC 
ACTGGAAAGA CTTACACAAT GGAAGGGGTA CGAAACGACC CGGAAAGAAG GGGTGTGATC 
CCAAACTCAT TTGAACACAT TTTCACACAC ATCTCCCGCT CTCAGAACCA GCAGTACTTG 
GTCAGGGCTT CATATTTGGA GATTTATCAG GAGGAGATCA GAGACCTTTT GTCAAAGGAC 
CAAGCACGTC GTTTGGAACT CAAAGAGCGA CCAGACACAG GTGTCTATGT TAAAGACCTG 
TCTTCGTTTG TCACTAAAAG TGTTCGTGAG ATTGAGCATG TGATGAATGT GGGGAACCAG 
AACCGCTCAG TTGGTGCAAC AAACATGAAT GAACACAGTT CTCGCTCTCA TGCCATCTTC 
GTCATCACCA TTGAGTGCAG TGAGTTGGGT CCTGACGGAG AGAACCATAT TCGAGTTGGG 
AAGCTTAACC TTGTTGACCT GGCAGGAAGT GAAAGACAGA CCAAAACCGG TGCTCAAGGT 
GAACGCTTAA AAGAAGCCAC TAAAATCAAT CTCTCCTTGT CAGCTTTGGG AAACGTCATC 
TCAGCGTTGG TGGATGGAAG AAGCACCCAT ATCCCGTACC GTGACTCTAA GCTTACAAGA 
CTTCTTCAAG ACTCCTTGGG TGGAAACGCC CGAACGGTTA TGGTGGCCAA CATTGGACCA 
GCTTCCTACA ATGTAGAGGA GACTCTAACG ACGCTACGCT ATGCAAACCG CGCCAAAAAC 
ATCAAGAACA AACCCCGTGT CAATGAAGAC CCCAAGGATG CCCTTCTTCG GGAGTTTCAG 
GAGGAGATTG CCCGCTTGAA AGAACAGTTG GAGAAAAGGT CTGGAAGGAA GAGGAGGAGA 
AGGAGGAGAA GAAGGGTTGG AGAAGGAGGA GAGGAGTTTG AGGATGGGGA GGATGAAGAG 
GATGATGATG ATGATGATGA AGACGAAGAG GAAGGAGTGG ATGCAGATAA GAACATTGCA 
GATTATTGGC ATGAGCAACA GGAGAAGCTG GAGAAGGAGA GGAGAGCCAT CATGGAGGAC 
CACAGTCTGG TGGCAGAGGA GAAACAGCGG CTGCTGAAGG AGAAGGAGAG GAAGATGACT 
GACCTGCACA AAGAGAAAGA GGCATCAGAG ATGCTGACAG CCAAAGTTAA GGCCATGGAG 
AGCAAGCTGC TGGTAGGAGG AAAGAACATA GTGGACCACA CCAACGAGCA GCAGAAGGTT 
CTGGAGCTGA AGAGACAGGA GATCGCAGAA CAGAAAAGGC GGGAAAGGGA GATGAAGCAA 
GAGATGGAGT GTCGTGACGA GGAGACACTG GAGCTAAAAG AGACGTACAG TTCACTGCAG 
CAGGAAGTTG ACATCAAGAC CAAGAAACTG AAAAAGCTCT TTTCCAAGCT TCAGTCTGTA 
AAGTCAGAGA TTCAGGATGC CCAGGATGAG CACGTGAAAT ACAGACAGGA ACTGGAACAG 
ACTCAGAATG AACTGACCAG AGAACTTAAA CTCAAACACT TAATCATAGA GAACTTCATC 
CCAATGGAGG AGAAGAACAA GATTGTAACA AGAGCCACAT TTGATGAAGA AGACGATCTC 
TGGAAAATGA CCCCCATCAC CCGTATACAA AATGATCATC AGATGATGAA AAGGCCTGTT 
TCTGCAGTGG GCTACAGACG GCCTCTGAGT CAGCATGCCC GTATGGCCAT GTTGATGCGG 
CCTGATGTCA GATACAAGGC TGAGAATATC TTGCTGCTGG AGCTGGATTT GCCCTCCCGG 
ACCACAAAAG ACTACGAGGG GCCAGTGATC GCACCTAAAG TAGCTGCAGC TCTGGAGGAC 
GCTCTGAGGG AGGAGGACGA AATCCAGGTG GACGCCTCTG GTTTTCATGC CAGCCTGGGC 
TCTAGTCCAG GCCTCAGTGC CTCAGCTGCT GGCTTTTCCA AGAAACCAAA GTCAGGCCGT 
CCAAAAACAG GCAAGAAGGT GAGCACTCCA ACTTCAGCCC ATAGCCCTCT GTCCGGCTCA 
GGGTCTCCTC TTTACCCACA ATCCCGAGGC CTTGTGCCTA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The cytoplasmic tail of rhodopsin triggers rapid rod degeneration in kinesin-2 mutants.
The Journal of biological chemistry292(42)17375-17386(2017 10)
Feng D,Chen Z,Yang K,Miao S,Xu B,Kang Y,Xie H,Zhao C

Function-driven discovery of disease genes in zebrafish using an integrated genomics big data resource.
Nucleic acids research44(20)9611-9623(2016 Nov)
Shim H,Kim JH,Kim CY,Hwang S,Kim H,Yang S,Lee JE,Lee I

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Differential roles for 3-OSTs in the regulation of cilia length and motility.
Development (Cambridge, England)140(18)3892-902(2013 Sep)
Neugebauer JM,Cadwallader AB,Amack JD,Bisgrove BW,Yost HJ

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KIF3B cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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