lama2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	lama2
Entrez Gene ID	100049705
Full Name	laminin, alpha 2
General protein information	Preferred Names laminin, alpha 2 Names laminin subunit alpha-2 caf candyfloss laminin alpha 2 chain unm teg15a unm tk209
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001278799.1	NP_001265728.1	laminin subunit alpha-2 precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Lama2	NP_032507.2
Rattus norvegicus (Norway rat)	Lama2	XP_219866.6
Homo sapiens (human)	LAMA2	NP_001073291.1
Pan troglodytes (chimpanzee)	LAMA2	XP_003311525.1
Bos taurus (cattle)	LAMA2	XP_002690266.1
Xenopus tropicalis (tropical clawed frog)	lama2	XP_002936356.2
	LAMA2	XP_001105600.2
Danio rerio (zebrafish)	lama2	NP_001265728.1
Gallus gallus (chicken)	LAMA2	XP_419746.4
Caenorhabditis elegans (roundworm)	lam-3	NP_492775.2
Canis lupus familiaris (dog)	LAMA2	XP_003432570.1

Gene Ontology
Bibliography

Related articles in PubMed

A novel early onset phenotype in a zebrafish model of merosin deficient congenital muscular dystrophy.
Smith SJ, Wang JC, Gupta VA, Dowling JJ
PloS one12(2)e0172648(2017)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Laminin and Matrix metalloproteinase 11 regulate Fibronectin levels in the zebrafish myotendinous junction.
Jenkins MH, Alrowaished SS, Goody MF, Crawford BD, Henry CA
Skeletal muscle618(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

analysis of the caf zebrafish model of MDC1A, which has a mutation in LAMA2
Title: A novel early onset phenotype in a zebrafish model of merosin deficient congenital muscular dystrophy.

splice site mutation in laminin-alpha2 results in a severe muscular dystrophy and growth abnormalities
Title: A splice site mutation in laminin-?2 results in a severe muscular dystrophy and growth abnormalities in zebrafish.

The degenerative muscle phenotype in the zebrafish dystrophic mutant, candyfloss (caf) results from mutations in the laminin alpha2 (lama2) gene.
Title: The zebrafish candyfloss mutant implicates extracellular matrix adhesion failure in laminin alpha2-deficient congenital muscular dystrophy.

The following lama2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lama2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa05305	NM_001278799.1 Latest version!	Danio rerio laminin, alpha 2 (lama2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lama2 ( NM_001278799.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001265728.1 Danio rerio laminin, alpha 2 (lama2), mRNA.

Price & Availability↑

CloneID	ODa05305
Clone ID Related Accession (Same CDS sequence)	NM_001278799.1
Accession Version	NM_001278799.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-31
Organism	Danio rerio(zebrafish)
Product	laminin subunit alpha-2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from JN786914.1. On Jul 17, 2013 this sequence version replaced XM_002665635.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: JN786914.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA898403, SAMN01765705 [ECO:0000350] ##Evidence-Data-END##

ATGGACACGC GGAGTTTAGT GGCGCTGCTC GGGCTGCTGT GGCGGTGTTG TGGAGCTCCG 
CAGAGGGGTC TTTTCCCAGC GGTGCTGAAT CTGGCCTCCA TGGCAGAGAT CAAGACCAAC 
GCCACATGTG GAGAAACAGG TCCAGAGATG TACTGCAAAC TAGTGGAGCA TGTGCCAGGA 
CGTCCCGTCA AGAACCCTCA GTGTAGGACC TGCGATCTGA ACAGCGATTA TGATTACGAG 
CGGCATCCTA TTGAGTTCGC CATCGATGGC ACAAACAGAT GGTGGCAGAG CCCAAGCATC 
ATGAACGGGA TGGACTTGCA TCACGTCACT GTCACTCTGG ACCTGCAGCA GGTTTTCCAG 
ATTGCTTATG TGATCCTGAA GGCTGCAAAC TCTCCTCGAC CTGGGAACTG GATTCTGGAG 
CGATCTCTGG ATGGTGAAAC CTTCATGCCG TGGCAATATT ACGCCATAAC AGACACAGAG 
TGTATAACAC GCTATGACAT CATCCCCAGG ACAGGCCCAC CATCCTACAC ACACGACGAC 
GAGGTGATCT GTACCTCCTT CTACTCCAAA ATACATCCTC TGGAGAACGG AGAAATCCAC 
ACATCCCTGA TAAACGGACG TCCCAGTGCA GACGATCCCT CGCCAACACT CTTGAACTTC 
ACCTCCGCAC GCTTCATCCG TCTGCAGTTC CAGAGAATCC GTACATTAAA TGCTGACCTC 
ATGACCCTCG CCCTGCACGA CCCGCGGGAC ATCGACCCTA TTGTCACCAG AAGGTATTAT 
TACTCCATTA AGGATATCTC CGTCGGAGGC ATGTGCATCT GTTACGGTCA CGCGAAGGCC 
TGCCCTCTCA ACACACACAC CAAGAAATTC AGCTGTGAGT GTGAGCACAA CACATGTGGA 
GAGAGCTGCG ACCGCTGCTG TCCTGCTTAC AACCAGAAGC CCTGGATGGC AGGAACCTTC 
CTCACACGGC ACGTCTGCGA AAAGTGTAAT TGTCACAATA AATCAGAGGA ATGCTATTAC 
AACCAGACGG TTGCCGATGC TAAGCTGAGT TTGAATATTC ATGGTGAATA TGAGGGGGGC 
GGAGTCTGCC TCGGCTGCTC TGAGAACACT GGAGGAATTA ACTGCCAATC ATGTATAGAC 
GGCTTCTACA GACCAAGTGG GGTGAGTCCA CTGGAGCCGT GGCCCTGCAC ACCCTGCACA 
TGTGACCTGC GAGGGGCACT TCACTCTACA TGCGTCCCTG ATGAGAGTCA GGCCACCACA 
GATCTGCCTG TGGGCTCGTG CATCTGTAAG CCGGGATACG CAGGTGAGCG CTGTGACAGG 
TGTGTGTTTG GATACGCTGG ATTCCCTCAG TGTGAGCGCT GCAGATGCAG CAGTGACGGC 
AGTGTAAATC AGGATCCCTG CCAGCCGCCC TGTGTGTGTA AGGAGCATGT GGAGGGACGC 
GACTGTGACC GCTGTAAACA GGGATTTTAC CATCTCAGCG GAGGACGAGT GCGCGGATGT 
GACAAGTGCT ACTGTTCTGG AGTCGCCAGC CTGTGTGCCG AATCCCAGTG GGACTACACT 
AATGTGACGG ATATGTCTGG ATGGTATCTG ACAGGAGCGG ATGGGGAGGG GCTTGTGTGG 
GCAGAGCCAA GCAGAGAGAC CGCCCATCAG GTTACTGTGA GCCAATCAGA TGCTCAGACT 
CACCTGACAG CACCTTACTA CTGGAGCGCC CCGTCTGCTT ACCTGGGCAA GAAGCTCTTG 
GCGTACGGAG GCAGGCTGGT GTATTCGATT TCTCACGACA CAGAAAGCCT GGAGGCCGTT 
CACACTCTCG GAGCTCCTGA TGTCATCATA GAGGGTGGAG GTCTGCGGCT GGTGCACAGG 
TGGTCTGAGG TGTTGCTGAA GTCGCCCTCC TCATCTGAGC GTGGCGTAGA TCTGCTCCCG 
GAGCTTTTCC AGCATCAGGC CTCAGGGCAG AGCGTCAGCA GGAGAGACTT CCACTCTGTG 
CTGCAGCACA TCCAGCGGCT GATGCTGCGG GCCGAGCACA GTCTACAGGA GCACACACAC 
GCCATCTACA GGCTGGGCTC TGTGAGGACG AGTGTGGCTG ATGAAGACTC TCGTAGTGGC 
ATTCAGGCCG GGGCTGTGGA GGTGTGCCAG TGTCCTCCCG GATATGATGG GACATCCTGC 
GAGATGTGTG CGCTGGGCTA CCGGCGGGTC AACGGGACGA TCTACCAGGG TGTGTGTGAA 
CTCTGTCAAT GCCACGGACA CACAGAGCGC TGTGATGACC TCACTGGACA CTGCCTGGGC 
TGCAGGGATC ACACTGTAGG ACCGAACTGT GGCCAGTGTG CTCCTGGATA TTATGGAGAC 
GCCACTAGAG GAACTGCAGA CGACTGTCAG CCCTGCGCCT GTCCTCTGCT GAGCCCCGCA 
AACAATTTCA GTCCCACATG TCGTGTGGAT GGCAGGGGCG AGGTGATCTG CGACCGATGC 
CCGCCGGGGT ACACCGGATC ACGCTGCGAC AGGTGTTCTA ATGGCTATTT CGGACGTCCC 
AATATTCCTG GCGGCTCCTG TCAGCCGTGT GTGTGTAGCG GTAACCTGGA CCAAACCGCT 
CCGCTGAGCT GTGACCCGCT GACCGGAGCC TGTCTGCGCT GTCGAAAGGG ATACGGAGGA 
GCTGACTGCG AACGCTGCGC TGACGGATAC TTCGGAGATG CCCTCATAGC CAAGAACTGC 
CAAGCGTGTC AGTGCCACAG CAATGGATCA GTGTCAGAGC TCTGCCATCA GGAGACAGGA 
CAGTGCCAGT GTCGCCAGCA CGTGATTGGA CGGCAGTGTG ACGAGTGTAT GGCTGGCTCT 
CATCTGCAGG GCGCTCTCGG CTGTGTCCCG TGCCACTGCA ACTCTTTTGG CTCCAAATCG 
TTTGACTGTG ATGAGAGCGG GCAGTGCCGC TGCCAGCCCG GTGTCACGGG ACAGAAGTGT 
GACCGCTGTG CCGCTGGACA CTTCAGCTTC CAGGAGGGAG GATGCACACC GTGCCAGTGT 
GCCCATGTGG GAAATAACTG CGACGCCAGC ACGGGCCAGT GTATCTGTCC GCCCAACACC 
GTAGGAGAGC GATGTGACAA ATGTGCGCCC AACCACTGGG GTCACGACAT CAGCACGGGC 
TGCAAGCCGT GTGGCTGTAA TGCACTGGGG TCTGTGGCGC AGCAGTGTAA CCTGAACACC 
GGCTGCTGCA TGTGCCGAGA GCAGTTCAGG GGGGAGAAAT GCGACGAGTG TAAGCTTGGA 
TACAGAGATT TCCCACAATG CATTTCCTGC CAGTGCTCTG CAGCAGGCTC ATCGCTGGAC 
ACCTGCGACT CAGAGTCAGG CCTCTGCGCA TGTGCGGACA GAAGCGGACA GTGCTCCTGC 
AAGGCTAATG TGGAGGGCCA GAAGTGTGAG CGCTGTAAGA GCGGCTCATT CGGCCTCAGT 
CACAGGAATC CTCTGGGCTG CAGTCGCTGC TACTGCTTTG GCTTGAGCAG CTCCTGTACA 
GAGGCCACAG GACTCATCCG AATGAGGCTG ACTCTGATGC CCGAGCAGAC TGTTCTTCCT 
CTGGTGGACC GTTCTGGACA TCAGGAGACC ACAGCAGGCA TCACTTTCCA GCATCCAGAC 
ATCATCGCCA CCGCAGACGT GGTCCAGCAG CAGCTGAGTG AGCCGTACTA CTGGAGACTG 
CCCAAACAGT TCAAGCGCTC CATGATAACG GCCTATGGTG GTAAGCTAAG GTACGCCATA 
TATTACGAGG CCCGTGAGGA GACAGGACGC ACCTCATACG AGCCTCAGGT CATCATTAAG 
GGTGGCCCGA ATAAAGACAA GATCATGGTG CGGCACATGC CTGCGCTTCA GATCGGCCAG 
CTGACCCGCC ATGAGATCGA CATTACAGAG CACGAGTGGA AACACAAGGA CGACAGGCCC 
ATGTCTCGAG AGGACTTCAT GGACGTTCTG TTTCATGTGG AATATATACT AATAAAGGCT 
TCGCACGGCA ACGTCATGAG GCACAGCAGG ATTTCTGAGA TCACTCTGGA GGTGGCGGAG 
GTGGGCAGAG CATCCCAGGA CAGTGAAATC GCCCATCAGA TTGAGAAATG TGTGTGCCCT 
CAGGGATACG CCGGTCTCTC CTGTGAGGAG TGTGCAGCCG GGTTCTACAG ACTCTCGGTT 
CTGGCTGGTG GATCTGCATC GCGGGCCGGT TTTGGCAGCT GCGTTCGCTG CCAGTGCAAT 
GGGCACAGCG ACTCCTGTGA CCCCGAAACC TCCATCTGCC AGAATTGTCA GCACAACACA 
GTGGGTGATA AGTGTGAGCG CTGTGCTGCG GGGTTTTACG GTGTAGTGCG AGGATTTACA 
GACGACTGCA AACCCTGCGC CTGTCCACTG CTCAACCCTG AGAACAACTT CAGTCCCACC 
TGTCAGACGG AGGGTTTTAA TGAGTACCGC TGCACCGCCT GTCCTGAAGG CTACGAGGGA 
AAACACTGTG AAAGGTGCGC TCCTGGTTTT CACGGTGACC CCGGGGTGCT GGGCGGCCGC 
TGTGAGGAGT CGTGTGACGA CGACTGCGCC GGGCTCTTGA TTCGGGACAT GGAGCGTTTG 
CTGCGTTTGA TTGGCAGTGT CAATCTCACT CTGCCCCTCC CACTGCCCTA TAAAGTGCTG 
TATCGATTCG AGAACGTGAC GGAGGAGCTG AAGCACATGC TGTCTCCTCA GAGAGCACCA 
GAGAGACTAA TGCAGCTGGC CGACAGCAAC CTGGGAAGCC TGGTCACTGA GATGGACGAA 
CTGCTTAACC GGGCAACTAA AGTGTCTGCT GATGGGGAGC AGACGGCAGC GGATGCAGAG 
AGAAGCCGTA AAGCTGCAGA AGAGCTGGAA CTGTACGTCA GGAACACACT GCTGGCTGCT 
GAAGCACTGC GTGACAAAGC ACGTGATCTG AACGCGACTT TGGGACTTCG GGATGGGACT 
TTAGAGAAAA GCGTGAACGA GATGAATGAC GAGATCCAGG AAATGCTCGC AGAGCTCCGC 
AAACGCCAGC TCACCGGAAA GAAACACATG GCTGATGAAG AGCTCGGGGC TGCGGAGGCT 
CTCTTGTCCC GCGTGAAGCG CTTGTTTGGA GACCCACAGC AGGCCACAGC GGACCTGAAG 
CAGGAGGTGG GACAGAAGAT GAGCGACCAC AAGCTGAAGC TGCAGGAGGC GCAGGAGCTG 
CTGGAGGAGG CGCTCACACA CACCAGACAG GCTGAAGACC TGACTCTCAA CAACCAGCTC 
AAACTGCAGC ATCTGCAGGG AAAGAGGGAT GCGGCCGTTA AACAGAAAGA ACAAACAGAG 
GGGGTCTTAT TGGAGGGAGA GAAGAGTCTG GACGAAGCCA ATGCTCTCTC CGACGCCATC 
ACCCGCGGCA AAGAGGAGCT GGAGGAGATG GCGGGAGAGC TGGGGCCTCT GCGGGATCAG 
CTGCGGGATA AAGAGTCTGA TCTGAGTCGC GGTCTGGATG AGTCTGTCCC GGAGCTGCTG 
CTAAGAGCGG AGGATCACGC CGCACAGCTC AACCACTCCG CCGCCATACT CGACAGTATT 
CTGGCTGAGG CAAAGAATCT GTCCTTCAAC GCCACGGCTG CTTTCAGCGC ATACACCAAC 
ATCAAGAACT ACATCGAGGA GGCCGATAGA GTGGCCAAAG ATGCAAAGAA AACCAGCGCT 
GAGGCTCTAC AGCTGGCCTC GGGACCCAAA GGCTCTCTGA AAGATCAGGC TAAAGGTTCG 
CTCCAGAAGA GCCACGATCT GCTGAATGAG GCTAAAGACC TGCAGAAGGA CGTGACAGAA 
AATGGAGAGA ATCTGGGAGC CCTTCAGCTC AGACTGAAAG GAGCCAACAA GCAGAACAAA 
GACCTACAGA AACGCCTGAA CGACACCGTG GAGAAACTCA ACTCCATCCC TGACGATATG 
GCGCTGAAGA TCCAGGCTAC TAAGCTGAAG GCGGCCGAAG CCAACGACAC GGCGCTGGAC 
GTCCTGAATC GGCTGAAGGA CATGAACCTC AACCTGATGG GCTTGAAGAA AAACTACGAC 
AAGCTGGAGG ACGACGTCAG GAAAACCAAC AACCTGATCA AAGACCCCGA GAAAAACATT 
CATGCAGCAG GCGCCAAAGT GAAGGATCTG GAGAATGAAG CCGACAGGCT GATGGAGAAG 
CTGAAGCCCA TTCAGGAGCT GCAGGACAAC CTGAAGAAAA ACATCTCGCA GATTAAAGAA 
CTCATCAACC AGGCACGCAA GCAGGCCAAC TCTATTAAGG TGTCTGTGTT GTCCGGTGGA 
GACTGCATCC GCACCTACCG GCCAGAGATC AAGAAGGGCC GCTACAACAC CATCATCCTC 
CACCTCAAGA CCCTCGCTGC CGATAACCTG CTCTTCTACC TGGGATCCGC CAAATACGTG 
GATTTCTTGG CCATCGAGAT GCGTAAGGGG AAGGTGAACT TCCTGTGGGA TGTGGGGTCA 
GGGGTCGGCC GAGTGGAATA TCCAGATCTG ATCATCAACG ATGGAAACTG GCACCGAATC 
GAAGCCTCAC GTATTGGTCT GAACGGCAGT ATCTCAGTCC GTGCGCTCGA GGGACCCAAA 
ACAGGCATCT TACCTTCACT CAGAAGTGCA AAAGCACCGG AGACCTACAC TGTGCTGGAC 
GTGGACCAGA ACGCTTACCT GTTTGTCGGA GGCATGCTGG GATCAGTGAA GAAAGCAGAT 
GCTGTGAAAA CCACCACATT CTCAGGGTGT ATGGGTGAGA CGTCTCTGGA TGGAAAGCCC 
ATCGGCCTCT GGAACTACAG AGAGAGAGAC GGGGACTGTG CTGGATGTGT TGTCAGTCCT 
CAGCCTGCAG ACACTGAAGG AACGGTTCAG TTTGATGGAG ATGGTTATGC GTCCGTCAGT 
CGCCCCACCA GGTGGAACCC AAATGTCTCC ACTGTCATGT TCAAGTTTCG CACCTTCTCC 
ACACACTCCG TCATCATGTA CTTCGCCACC AAAGACATGA AAGACTTCAT GAGTGCTGAA 
CTGAGTGATG GCAGGGTGAA AGTGAGTTAT GATCTGGGCT CTGGCACCGG CTCCATCATC 
AGTGATAAAC GCTATAATGA CGGCAAGTGG AAGTCCTTCA CCATGTCCCG CATGAAGAAA 
GAAGCCACCA TCACCATCGT CAACATGGAC ACTAATGAGA ATGAGCGGCT GCTGATGGCG 
TCACCAGGGG GCGCTACAGG CCTTAACCTG CGAGACAACG AGCGCATATT TTTCGGAGGA 
TTGCCCAAAG CTGGAAAATA CAGGTCAGAG GTGGTTTTGA AGAGGTTTTC TGGCTGTATG 
AAGGATATCG AGGTCTCCCG AACGCCATAT AATCTGCTGA GCAGTCCAGA TTACACAGGA 
CTGACCAAAG GCTGCTCCAT TGAGAATCTG CACACAGTGA GTTTCCCGCG GGCCGGGTTC 
ATGGAGCTGA ATCCGGTGTC ATTTGCCGTG GGCTCGGAGA TCTCGCTCTC CTTCAGCACC 
AGGAGTGAAG ACGGGCTGAT ACTGTTCGGC AGAGGAGGAC TGACCTCCAT GACGGAGCTG 
ACACAGGGAC TCACGCCAGT GCACATCCCA CGCCGCTCAC GCAGACAGAC CGGAGAGCCC 
TTCCTCTCTG TGCAGCTCAG TAAAGGGGCA CTGGAGGTGC TGGTGTTCAC CGGCTCCAGA 
AACCCTCGCA GAGTTTTCCG AAAACCAGAT CAGGGAATCC TGCATGATGG CCGAGAGCAC 
TCACTGCGCA TCCAGAGGCT CCCGGGAGCA TCCTTCACTG TGCAGGTGGA CGAGGAGCCG 
CTGGTGCAGC AGCCCTTACC CAACGACCTC CCACTCAACA TCCACCGCCT CTTCATCGGA 
GGAATCCCGC CTGATGTACA AACCGGCGTC CACAGGCCCA GCGTGCCGTT CGAGGGCTGC 
ATCTGGAACC TCCTGATCAA CACTGTCCCG GTGGACTTCT CCCAGCCTGT GGCCTTTGAG 
AATGCAGAGA TTGGCCACTG CCCTGACCTG GCCCCGGCCC CAGCTCCGCC TCTTGAGGAG 
GTGGAAAAGG AAGAGGCTCC GCCTACACCA GCAGAAGCAG TTCGTCCTCC ACCAGCTCCA 
GCCTCCACTG CCACACCCCT TGCCGAAGCC AAGCCCATCA CAGACACACC CACCAGTGTC 
AAAGAGCCCG CGGTGTCGTG CGCAGCAGAG GCGGAGCCTG CAGTGCTGGG GCTGGCCAAA 
CAGTTCGGTC TGTCCAGAAA CAGTCACATG AGCTTTGAGT TTGATGACAG AAAGGTGATG 
AACAGGTTGG TCATTGAGTT CGAGATGCGC ACAGAGACAG ACTCTGGTCT GGTTTTCTAC 
ATGGCCAGAA TAAACCACGC AGACTTCGCC ACCATCCAGC TGAAGGAGGG AATGGCTCAC 
CTAAGCTTCG ACCTGGGCAG CGGGAACACC TCCGTCAGCG TGCCGCGCAT CATCAACGAC 
GGGCAGTGGC ACAAGATCCG TGTGATGAGA GACAAACAGA GAGGAGTCCT CACGATAGAC 
GGCCGCTACA GCAAACACAC CACCAGCCCG AAGAAAGCAG AAATCCTGGA TGTGGTGGGA 
ATGCTGTATG TTGGTGGTTT ACCGCTCAAC TACACCACCA AACGCATCGG CCCTGTGCTG 
TACAGTATTG ACGCTTGCAT TAGGAACTTC AAGATGATGA ATCTGCCTTT GGATATGGAG 
AATCCCACCT CCAGCTACCG CGTGGGCTCC TGCTTTGCCA ACCCAGAAAA GGGAAACTAT 
TTCAATGGCA CAGGCTATGC CAAAGCTGTG GAGGCATATC GAGTGGGTAA TGACGTGTCA 
GTGGATCTGG AGTTCAGGAC GGCTCAGAGC TCTGCTGTCC TGGTGGGCGT CAGCAGCCAA 
AAGATGGACG GGCTGGGCAT CGAACTCGTA AATGGAAAGC TGTTCTTCCA TGCTGATAAC 
GGAGTGGGCC GCTTCACAGC CGTGCATGAG CCGAGGCAGG AGGCGGGGCT TTGTGATGGG 
CAGTGGCACA CAGTCTCCGC CCACAAGCTG AAGCACCGCC TGGAGCTGAC GGTGGACGGA 
CAGAAGTCTG AGGCGGCGAG TCCAGACATC CGCTCGCCCT CCGCAGACAC CAATGACCCA 
CTGTATGTGG GCGGATACCC CGAGGGTCTG AAGCAGTTCG GGCTCACCAT AAACACCCCG 
TTTAAAGGCT GCATGAGGAA CGTGAAGATC AGTAAAGCTG GAAAAACTCT GCAGGTCCAG 
ATGAACAAAC TGCTGGAGCT CAGAGGAGTT CAGCCGCTCA GCTGCCCTGC TGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265728.1
CDS	1..9237
Translation	MDTRSLVALLGLLWRCCGAPQRGLFPAVLNLASMAEIKTNATCGETGPEMYCKLVEHVPGRPVKNPQCRTCDLNSDYDYERHPIEFAIDGTNRWWQSPSIMNGMDLHHVTVTLDLQQVFQIAYVILKAANSPRPGNWILERSLDGETFMPWQYYAITDTECITRYDIIPRTGPPSYTHDDEVICTSFYSKIHPLENGEIHTSLINGRPSADDPSPTLLNFTSARFIRLQFQRIRTLNADLMTLALHDPRDIDPIVTRRYYYSIKDISVGGMCICYGHAKACPLNTHTKKFSCECEHNTCGESCDRCCPAYNQKPWMAGTFLTRHVCEKCNCHNKSEECYYNQTVADAKLSLNIHGEYEGGGVCLGCSENTGGINCQSCIDGFYRPSGVSPLEPWPCTPCTCDLRGALHSTCVPDESQATTDLPVGSCICKPGYAGERCDRCVFGYAGFPQCERCRCSSDGSVNQDPCQPPCVCKEHVEGRDCDRCKQGFYHLSGGRVRGCDKCYCSGVASLCAESQWDYTNVTDMSGWYLTGADGEGLVWAEPSRETAHQVTVSQSDAQTHLTAPYYWSAPSAYLGKKLLAYGGRLVYSISHDTESLEAVHTLGAPDVIIEGGGLRLVHRWSEVLLKSPSSSERGVDLLPELFQHQASGQSVSRRDFHSVLQHIQRLMLRAEHSLQEHTHAIYRLGSVRTSVADEDSRSGIQAGAVEVCQCPPGYDGTSCEMCALGYRRVNGTIYQGVCELCQCHGHTERCDDLTGHCLGCRDHTVGPNCGQCAPGYYGDATRGTADDCQPCACPLLSPANNFSPTCRVDGRGEVICDRCPPGYTGSRCDRCSNGYFGRPNIPGGSCQPCVCSGNLDQTAPLSCDPLTGACLRCRKGYGGADCERCADGYFGDALIAKNCQACQCHSNGSVSELCHQETGQCQCRQHVIGRQCDECMAGSHLQGALGCVPCHCNSFGSKSFDCDESGQCRCQPGVTGQKCDRCAAGHFSFQEGGCTPCQCAHVGNNCDASTGQCICPPNTVGERCDKCAPNHWGHDISTGCKPCGCNALGSVAQQCNLNTGCCMCREQFRGEKCDECKLGYRDFPQCISCQCSAAGSSLDTCDSESGLCACADRSGQCSCKANVEGQKCERCKSGSFGLSHRNPLGCSRCYCFGLSSSCTEATGLIRMRLTLMPEQTVLPLVDRSGHQETTAGITFQHPDIIATADVVQQQLSEPYYWRLPKQFKRSMITAYGGKLRYAIYYEAREETGRTSYEPQVIIKGGPNKDKIMVRHMPALQIGQLTRHEIDITEHEWKHKDDRPMSREDFMDVLFHVEYILIKASHGNVMRHSRISEITLEVAEVGRASQDSEIAHQIEKCVCPQGYAGLSCEECAAGFYRLSVLAGGSASRAGFGSCVRCQCNGHSDSCDPETSICQNCQHNTVGDKCERCAAGFYGVVRGFTDDCKPCACPLLNPENNFSPTCQTEGFNEYRCTACPEGYEGKHCERCAPGFHGDPGVLGGRCEESCDDDCAGLLIRDMERLLRLIGSVNLTLPLPLPYKVLYRFENVTEELKHMLSPQRAPERLMQLADSNLGSLVTEMDELLNRATKVSADGEQTAADAERSRKAAEELELYVRNTLLAAEALRDKARDLNATLGLRDGTLEKSVNEMNDEIQEMLAELRKRQLTGKKHMADEELGAAEALLSRVKRLFGDPQQATADLKQEVGQKMSDHKLKLQEAQELLEEALTHTRQAEDLTLNNQLKLQHLQGKRDAAVKQKEQTEGVLLEGEKSLDEANALSDAITRGKEELEEMAGELGPLRDQLRDKESDLSRGLDESVPELLLRAEDHAAQLNHSAAILDSILAEAKNLSFNATAAFSAYTNIKNYIEEADRVAKDAKKTSAEALQLASGPKGSLKDQAKGSLQKSHDLLNEAKDLQKDVTENGENLGALQLRLKGANKQNKDLQKRLNDTVEKLNSIPDDMALKIQATKLKAAEANDTALDVLNRLKDMNLNLMGLKKNYDKLEDDVRKTNNLIKDPEKNIHAAGAKVKDLENEADRLMEKLKPIQELQDNLKKNISQIKELINQARKQANSIKVSVLSGGDCIRTYRPEIKKGRYNTIILHLKTLAADNLLFYLGSAKYVDFLAIEMRKGKVNFLWDVGSGVGRVEYPDLIINDGNWHRIEASRIGLNGSISVRALEGPKTGILPSLRSAKAPETYTVLDVDQNAYLFVGGMLGSVKKADAVKTTTFSGCMGETSLDGKPIGLWNYRERDGDCAGCVVSPQPADTEGTVQFDGDGYASVSRPTRWNPNVSTVMFKFRTFSTHSVIMYFATKDMKDFMSAELSDGRVKVSYDLGSGTGSIISDKRYNDGKWKSFTMSRMKKEATITIVNMDTNENERLLMASPGGATGLNLRDNERIFFGGLPKAGKYRSEVVLKRFSGCMKDIEVSRTPYNLLSSPDYTGLTKGCSIENLHTVSFPRAGFMELNPVSFAVGSEISLSFSTRSEDGLILFGRGGLTSMTELTQGLTPVHIPRRSRRQTGEPFLSVQLSKGALEVLVFTGSRNPRRVFRKPDQGILHDGREHSLRIQRLPGASFTVQVDEEPLVQQPLPNDLPLNIHRLFIGGIPPDVQTGVHRPSVPFEGCIWNLLINTVPVDFSQPVAFENAEIGHCPDLAPAPAPPLEEVEKEEAPPTPAEAVRPPPAPASTATPLAEAKPITDTPTSVKEPAVSCAAEAEPAVLGLAKQFGLSRNSHMSFEFDDRKVMNRLVIEFEMRTETDSGLVFYMARINHADFATIQLKEGMAHLSFDLGSGNTSVSVPRIINDGQWHKIRVMRDKQRGVLTIDGRYSKHTTSPKKAEILDVVGMLYVGGLPLNYTTKRIGPVLYSIDACIRNFKMMNLPLDMENPTSSYRVGSCFANPEKGNYFNGTGYAKAVEAYRVGNDVSVDLEFRTAQSSAVLVGVSSQKMDGLGIELVNGKLFFHADNGVGRFTAVHEPRQEAGLCDGQWHTVSAHKLKHRLELTVDGQKSEAASPDIRSPSADTNDPLYVGGYPEGLKQFGLTINTPFKGCMRNVKISKAGKTLQVQMNKLLELRGVQPLSCPAA

Target ORF information:

RefSeq Version	NM_001278799.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio laminin, alpha 2 (lama2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278799.1

ORF Insert Sequence:

ATGGACACGC GGAGTTTAGT GGCGCTGCTC GGGCTGCTGT GGCGGTGTTG TGGAGCTCCG 
CAGAGGGGTC TTTTCCCAGC GGTGCTGAAT CTGGCCTCCA TGGCAGAGAT CAAGACCAAC 
GCCACATGTG GAGAAACAGG TCCAGAGATG TACTGCAAAC TAGTGGAGCA TGTGCCAGGA 
CGTCCCGTCA AGAACCCTCA GTGTAGGACC TGCGATCTGA ACAGCGATTA TGATTACGAG 
CGGCATCCTA TTGAGTTCGC CATCGATGGC ACAAACAGAT GGTGGCAGAG CCCAAGCATC 
ATGAACGGGA TGGACTTGCA TCACGTCACT GTCACTCTGG ACCTGCAGCA GGTTTTCCAG 
ATTGCTTATG TGATCCTGAA GGCTGCAAAC TCTCCTCGAC CTGGGAACTG GATTCTGGAG 
CGATCTCTGG ATGGTGAAAC CTTCATGCCG TGGCAATATT ACGCCATAAC AGACACAGAG 
TGTATAACAC GCTATGACAT CATCCCCAGG ACAGGCCCAC CATCCTACAC ACACGACGAC 
GAGGTGATCT GTACCTCCTT CTACTCCAAA ATACATCCTC TGGAGAACGG AGAAATCCAC 
ACATCCCTGA TAAACGGACG TCCCAGTGCA GACGATCCCT CGCCAACACT CTTGAACTTC 
ACCTCCGCAC GCTTCATCCG TCTGCAGTTC CAGAGAATCC GTACATTAAA TGCTGACCTC 
ATGACCCTCG CCCTGCACGA CCCGCGGGAC ATCGACCCTA TTGTCACCAG AAGGTATTAT 
TACTCCATTA AGGATATCTC CGTCGGAGGC ATGTGCATCT GTTACGGTCA CGCGAAGGCC 
TGCCCTCTCA ACACACACAC CAAGAAATTC AGCTGTGAGT GTGAGCACAA CACATGTGGA 
GAGAGCTGCG ACCGCTGCTG TCCTGCTTAC AACCAGAAGC CCTGGATGGC AGGAACCTTC 
CTCACACGGC ACGTCTGCGA AAAGTGTAAT TGTCACAATA AATCAGAGGA ATGCTATTAC 
AACCAGACGG TTGCCGATGC TAAGCTGAGT TTGAATATTC ATGGTGAATA TGAGGGGGGC 
GGAGTCTGCC TCGGCTGCTC TGAGAACACT GGAGGAATTA ACTGCCAATC ATGTATAGAC 
GGCTTCTACA GACCAAGTGG GGTGAGTCCA CTGGAGCCGT GGCCCTGCAC ACCCTGCACA 
TGTGACCTGC GAGGGGCACT TCACTCTACA TGCGTCCCTG ATGAGAGTCA GGCCACCACA 
GATCTGCCTG TGGGCTCGTG CATCTGTAAG CCGGGATACG CAGGTGAGCG CTGTGACAGG 
TGTGTGTTTG GATACGCTGG ATTCCCTCAG TGTGAGCGCT GCAGATGCAG CAGTGACGGC 
AGTGTAAATC AGGATCCCTG CCAGCCGCCC TGTGTGTGTA AGGAGCATGT GGAGGGACGC 
GACTGTGACC GCTGTAAACA GGGATTTTAC CATCTCAGCG GAGGACGAGT GCGCGGATGT 
GACAAGTGCT ACTGTTCTGG AGTCGCCAGC CTGTGTGCCG AATCCCAGTG GGACTACACT 
AATGTGACGG ATATGTCTGG ATGGTATCTG ACAGGAGCGG ATGGGGAGGG GCTTGTGTGG 
GCAGAGCCAA GCAGAGAGAC CGCCCATCAG GTTACTGTGA GCCAATCAGA TGCTCAGACT 
CACCTGACAG CACCTTACTA CTGGAGCGCC CCGTCTGCTT ACCTGGGCAA GAAGCTCTTG 
GCGTACGGAG GCAGGCTGGT GTATTCGATT TCTCACGACA CAGAAAGCCT GGAGGCCGTT 
CACACTCTCG GAGCTCCTGA TGTCATCATA GAGGGTGGAG GTCTGCGGCT GGTGCACAGG 
TGGTCTGAGG TGTTGCTGAA GTCGCCCTCC TCATCTGAGC GTGGCGTAGA TCTGCTCCCG 
GAGCTTTTCC AGCATCAGGC CTCAGGGCAG AGCGTCAGCA GGAGAGACTT CCACTCTGTG 
CTGCAGCACA TCCAGCGGCT GATGCTGCGG GCCGAGCACA GTCTACAGGA GCACACACAC 
GCCATCTACA GGCTGGGCTC TGTGAGGACG AGTGTGGCTG ATGAAGACTC TCGTAGTGGC 
ATTCAGGCCG GGGCTGTGGA GGTGTGCCAG TGTCCTCCCG GATATGATGG GACATCCTGC 
GAGATGTGTG CGCTGGGCTA CCGGCGGGTC AACGGGACGA TCTACCAGGG TGTGTGTGAA 
CTCTGTCAAT GCCACGGACA CACAGAGCGC TGTGATGACC TCACTGGACA CTGCCTGGGC 
TGCAGGGATC ACACTGTAGG ACCGAACTGT GGCCAGTGTG CTCCTGGATA TTATGGAGAC 
GCCACTAGAG GAACTGCAGA CGACTGTCAG CCCTGCGCCT GTCCTCTGCT GAGCCCCGCA 
AACAATTTCA GTCCCACATG TCGTGTGGAT GGCAGGGGCG AGGTGATCTG CGACCGATGC 
CCGCCGGGGT ACACCGGATC ACGCTGCGAC AGGTGTTCTA ATGGCTATTT CGGACGTCCC 
AATATTCCTG GCGGCTCCTG TCAGCCGTGT GTGTGTAGCG GTAACCTGGA CCAAACCGCT 
CCGCTGAGCT GTGACCCGCT GACCGGAGCC TGTCTGCGCT GTCGAAAGGG ATACGGAGGA 
GCTGACTGCG AACGCTGCGC TGACGGATAC TTCGGAGATG CCCTCATAGC CAAGAACTGC 
CAAGCGTGTC AGTGCCACAG CAATGGATCA GTGTCAGAGC TCTGCCATCA GGAGACAGGA 
CAGTGCCAGT GTCGCCAGCA CGTGATTGGA CGGCAGTGTG ACGAGTGTAT GGCTGGCTCT 
CATCTGCAGG GCGCTCTCGG CTGTGTCCCG TGCCACTGCA ACTCTTTTGG CTCCAAATCG 
TTTGACTGTG ATGAGAGCGG GCAGTGCCGC TGCCAGCCCG GTGTCACGGG ACAGAAGTGT 
GACCGCTGTG CCGCTGGACA CTTCAGCTTC CAGGAGGGAG GATGCACACC GTGCCAGTGT 
GCCCATGTGG GAAATAACTG CGACGCCAGC ACGGGCCAGT GTATCTGTCC GCCCAACACC 
GTAGGAGAGC GATGTGACAA ATGTGCGCCC AACCACTGGG GTCACGACAT CAGCACGGGC 
TGCAAGCCGT GTGGCTGTAA TGCACTGGGG TCTGTGGCGC AGCAGTGTAA CCTGAACACC 
GGCTGCTGCA TGTGCCGAGA GCAGTTCAGG GGGGAGAAAT GCGACGAGTG TAAGCTTGGA 
TACAGAGATT TCCCACAATG CATTTCCTGC CAGTGCTCTG CAGCAGGCTC ATCGCTGGAC 
ACCTGCGACT CAGAGTCAGG CCTCTGCGCA TGTGCGGACA GAAGCGGACA GTGCTCCTGC 
AAGGCTAATG TGGAGGGCCA GAAGTGTGAG CGCTGTAAGA GCGGCTCATT CGGCCTCAGT 
CACAGGAATC CTCTGGGCTG CAGTCGCTGC TACTGCTTTG GCTTGAGCAG CTCCTGTACA 
GAGGCCACAG GACTCATCCG AATGAGGCTG ACTCTGATGC CCGAGCAGAC TGTTCTTCCT 
CTGGTGGACC GTTCTGGACA TCAGGAGACC ACAGCAGGCA TCACTTTCCA GCATCCAGAC 
ATCATCGCCA CCGCAGACGT GGTCCAGCAG CAGCTGAGTG AGCCGTACTA CTGGAGACTG 
CCCAAACAGT TCAAGCGCTC CATGATAACG GCCTATGGTG GTAAGCTAAG GTACGCCATA 
TATTACGAGG CCCGTGAGGA GACAGGACGC ACCTCATACG AGCCTCAGGT CATCATTAAG 
GGTGGCCCGA ATAAAGACAA GATCATGGTG CGGCACATGC CTGCGCTTCA GATCGGCCAG 
CTGACCCGCC ATGAGATCGA CATTACAGAG CACGAGTGGA AACACAAGGA CGACAGGCCC 
ATGTCTCGAG AGGACTTCAT GGACGTTCTG TTTCATGTGG AATATATACT AATAAAGGCT 
TCGCACGGCA ACGTCATGAG GCACAGCAGG ATTTCTGAGA TCACTCTGGA GGTGGCGGAG 
GTGGGCAGAG CATCCCAGGA CAGTGAAATC GCCCATCAGA TTGAGAAATG TGTGTGCCCT 
CAGGGATACG CCGGTCTCTC CTGTGAGGAG TGTGCAGCCG GGTTCTACAG ACTCTCGGTT 
CTGGCTGGTG GATCTGCATC GCGGGCCGGT TTTGGCAGCT GCGTTCGCTG CCAGTGCAAT 
GGGCACAGCG ACTCCTGTGA CCCCGAAACC TCCATCTGCC AGAATTGTCA GCACAACACA 
GTGGGTGATA AGTGTGAGCG CTGTGCTGCG GGGTTTTACG GTGTAGTGCG AGGATTTACA 
GACGACTGCA AACCCTGCGC CTGTCCACTG CTCAACCCTG AGAACAACTT CAGTCCCACC 
TGTCAGACGG AGGGTTTTAA TGAGTACCGC TGCACCGCCT GTCCTGAAGG CTACGAGGGA 
AAACACTGTG AAAGGTGCGC TCCTGGTTTT CACGGTGACC CCGGGGTGCT GGGCGGCCGC 
TGTGAGGAGT CGTGTGACGA CGACTGCGCC GGGCTCTTGA TTCGGGACAT GGAGCGTTTG 
CTGCGTTTGA TTGGCAGTGT CAATCTCACT CTGCCCCTCC CACTGCCCTA TAAAGTGCTG 
TATCGATTCG AGAACGTGAC GGAGGAGCTG AAGCACATGC TGTCTCCTCA GAGAGCACCA 
GAGAGACTAA TGCAGCTGGC CGACAGCAAC CTGGGAAGCC TGGTCACTGA GATGGACGAA 
CTGCTTAACC GGGCAACTAA AGTGTCTGCT GATGGGGAGC AGACGGCAGC GGATGCAGAG 
AGAAGCCGTA AAGCTGCAGA AGAGCTGGAA CTGTACGTCA GGAACACACT GCTGGCTGCT 
GAAGCACTGC GTGACAAAGC ACGTGATCTG AACGCGACTT TGGGACTTCG GGATGGGACT 
TTAGAGAAAA GCGTGAACGA GATGAATGAC GAGATCCAGG AAATGCTCGC AGAGCTCCGC 
AAACGCCAGC TCACCGGAAA GAAACACATG GCTGATGAAG AGCTCGGGGC TGCGGAGGCT 
CTCTTGTCCC GCGTGAAGCG CTTGTTTGGA GACCCACAGC AGGCCACAGC GGACCTGAAG 
CAGGAGGTGG GACAGAAGAT GAGCGACCAC AAGCTGAAGC TGCAGGAGGC GCAGGAGCTG 
CTGGAGGAGG CGCTCACACA CACCAGACAG GCTGAAGACC TGACTCTCAA CAACCAGCTC 
AAACTGCAGC ATCTGCAGGG AAAGAGGGAT GCGGCCGTTA AACAGAAAGA ACAAACAGAG 
GGGGTCTTAT TGGAGGGAGA GAAGAGTCTG GACGAAGCCA ATGCTCTCTC CGACGCCATC 
ACCCGCGGCA AAGAGGAGCT GGAGGAGATG GCGGGAGAGC TGGGGCCTCT GCGGGATCAG 
CTGCGGGATA AAGAGTCTGA TCTGAGTCGC GGTCTGGATG AGTCTGTCCC GGAGCTGCTG 
CTAAGAGCGG AGGATCACGC CGCACAGCTC AACCACTCCG CCGCCATACT CGACAGTATT 
CTGGCTGAGG CAAAGAATCT GTCCTTCAAC GCCACGGCTG CTTTCAGCGC ATACACCAAC 
ATCAAGAACT ACATCGAGGA GGCCGATAGA GTGGCCAAAG ATGCAAAGAA AACCAGCGCT 
GAGGCTCTAC AGCTGGCCTC GGGACCCAAA GGCTCTCTGA AAGATCAGGC TAAAGGTTCG 
CTCCAGAAGA GCCACGATCT GCTGAATGAG GCTAAAGACC TGCAGAAGGA CGTGACAGAA 
AATGGAGAGA ATCTGGGAGC CCTTCAGCTC AGACTGAAAG GAGCCAACAA GCAGAACAAA 
GACCTACAGA AACGCCTGAA CGACACCGTG GAGAAACTCA ACTCCATCCC TGACGATATG 
GCGCTGAAGA TCCAGGCTAC TAAGCTGAAG GCGGCCGAAG CCAACGACAC GGCGCTGGAC 
GTCCTGAATC GGCTGAAGGA CATGAACCTC AACCTGATGG GCTTGAAGAA AAACTACGAC 
AAGCTGGAGG ACGACGTCAG GAAAACCAAC AACCTGATCA AAGACCCCGA GAAAAACATT 
CATGCAGCAG GCGCCAAAGT GAAGGATCTG GAGAATGAAG CCGACAGGCT GATGGAGAAG 
CTGAAGCCCA TTCAGGAGCT GCAGGACAAC CTGAAGAAAA ACATCTCGCA GATTAAAGAA 
CTCATCAACC AGGCACGCAA GCAGGCCAAC TCTATTAAGG TGTCTGTGTT GTCCGGTGGA 
GACTGCATCC GCACCTACCG GCCAGAGATC AAGAAGGGCC GCTACAACAC CATCATCCTC 
CACCTCAAGA CCCTCGCTGC CGATAACCTG CTCTTCTACC TGGGATCCGC CAAATACGTG 
GATTTCTTGG CCATCGAGAT GCGTAAGGGG AAGGTGAACT TCCTGTGGGA TGTGGGGTCA 
GGGGTCGGCC GAGTGGAATA TCCAGATCTG ATCATCAACG ATGGAAACTG GCACCGAATC 
GAAGCCTCAC GTATTGGTCT GAACGGCAGT ATCTCAGTCC GTGCGCTCGA GGGACCCAAA 
ACAGGCATCT TACCTTCACT CAGAAGTGCA AAAGCACCGG AGACCTACAC TGTGCTGGAC 
GTGGACCAGA ACGCTTACCT GTTTGTCGGA GGCATGCTGG GATCAGTGAA GAAAGCAGAT 
GCTGTGAAAA CCACCACATT CTCAGGGTGT ATGGGTGAGA CGTCTCTGGA TGGAAAGCCC 
ATCGGCCTCT GGAACTACAG AGAGAGAGAC GGGGACTGTG CTGGATGTGT TGTCAGTCCT 
CAGCCTGCAG ACACTGAAGG AACGGTTCAG TTTGATGGAG ATGGTTATGC GTCCGTCAGT 
CGCCCCACCA GGTGGAACCC AAATGTCTCC ACTGTCATGT TCAAGTTTCG CACCTTCTCC 
ACACACTCCG TCATCATGTA CTTCGCCACC AAAGACATGA AAGACTTCAT GAGTGCTGAA 
CTGAGTGATG GCAGGGTGAA AGTGAGTTAT GATCTGGGCT CTGGCACCGG CTCCATCATC 
AGTGATAAAC GCTATAATGA CGGCAAGTGG AAGTCCTTCA CCATGTCCCG CATGAAGAAA 
GAAGCCACCA TCACCATCGT CAACATGGAC ACTAATGAGA ATGAGCGGCT GCTGATGGCG 
TCACCAGGGG GCGCTACAGG CCTTAACCTG CGAGACAACG AGCGCATATT TTTCGGAGGA 
TTGCCCAAAG CTGGAAAATA CAGGTCAGAG GTGGTTTTGA AGAGGTTTTC TGGCTGTATG 
AAGGATATCG AGGTCTCCCG AACGCCATAT AATCTGCTGA GCAGTCCAGA TTACACAGGA 
CTGACCAAAG GCTGCTCCAT TGAGAATCTG CACACAGTGA GTTTCCCGCG GGCCGGGTTC 
ATGGAGCTGA ATCCGGTGTC ATTTGCCGTG GGCTCGGAGA TCTCGCTCTC CTTCAGCACC 
AGGAGTGAAG ACGGGCTGAT ACTGTTCGGC AGAGGAGGAC TGACCTCCAT GACGGAGCTG 
ACACAGGGAC TCACGCCAGT GCACATCCCA CGCCGCTCAC GCAGACAGAC CGGAGAGCCC 
TTCCTCTCTG TGCAGCTCAG TAAAGGGGCA CTGGAGGTGC TGGTGTTCAC CGGCTCCAGA 
AACCCTCGCA GAGTTTTCCG AAAACCAGAT CAGGGAATCC TGCATGATGG CCGAGAGCAC 
TCACTGCGCA TCCAGAGGCT CCCGGGAGCA TCCTTCACTG TGCAGGTGGA CGAGGAGCCG 
CTGGTGCAGC AGCCCTTACC CAACGACCTC CCACTCAACA TCCACCGCCT CTTCATCGGA 
GGAATCCCGC CTGATGTACA AACCGGCGTC CACAGGCCCA GCGTGCCGTT CGAGGGCTGC 
ATCTGGAACC TCCTGATCAA CACTGTCCCG GTGGACTTCT CCCAGCCTGT GGCCTTTGAG 
AATGCAGAGA TTGGCCACTG CCCTGACCTG GCCCCGGCCC CAGCTCCGCC TCTTGAGGAG 
GTGGAAAAGG AAGAGGCTCC GCCTACACCA GCAGAAGCAG TTCGTCCTCC ACCAGCTCCA 
GCCTCCACTG CCACACCCCT TGCCGAAGCC AAGCCCATCA CAGACACACC CACCAGTGTC 
AAAGAGCCCG CGGTGTCGTG CGCAGCAGAG GCGGAGCCTG CAGTGCTGGG GCTGGCCAAA 
CAGTTCGGTC TGTCCAGAAA CAGTCACATG AGCTTTGAGT TTGATGACAG AAAGGTGATG 
AACAGGTTGG TCATTGAGTT CGAGATGCGC ACAGAGACAG ACTCTGGTCT GGTTTTCTAC 
ATGGCCAGAA TAAACCACGC AGACTTCGCC ACCATCCAGC TGAAGGAGGG AATGGCTCAC 
CTAAGCTTCG ACCTGGGCAG CGGGAACACC TCCGTCAGCG TGCCGCGCAT CATCAACGAC 
GGGCAGTGGC ACAAGATCCG TGTGATGAGA GACAAACAGA GAGGAGTCCT CACGATAGAC 
GGCCGCTACA GCAAACACAC CACCAGCCCG AAGAAAGCAG AAATCCTGGA TGTGGTGGGA 
ATGCTGTATG TTGGTGGTTT ACCGCTCAAC TACACCACCA AACGCATCGG CCCTGTGCTG 
TACAGTATTG ACGCTTGCAT TAGGAACTTC AAGATGATGA ATCTGCCTTT GGATATGGAG 
AATCCCACCT CCAGCTACCG CGTGGGCTCC TGCTTTGCCA ACCCAGAAAA GGGAAACTAT 
TTCAATGGCA CAGGCTATGC CAAAGCTGTG GAGGCATATC GAGTGGGTAA TGACGTGTCA 
GTGGATCTGG AGTTCAGGAC GGCTCAGAGC TCTGCTGTCC TGGTGGGCGT CAGCAGCCAA 
AAGATGGACG GGCTGGGCAT CGAACTCGTA AATGGAAAGC TGTTCTTCCA TGCTGATAAC 
GGAGTGGGCC GCTTCACAGC CGTGCATGAG CCGAGGCAGG AGGCGGGGCT TTGTGATGGG 
CAGTGGCACA CAGTCTCCGC CCACAAGCTG AAGCACCGCC TGGAGCTGAC GGTGGACGGA 
CAGAAGTCTG AGGCGGCGAG TCCAGACATC CGCTCGCCCT CCGCAGACAC CAATGACCCA 
CTGTATGTGG GCGGATACCC CGAGGGTCTG AAGCAGTTCG GGCTCACCAT AAACACCCCG 
TTTAAAGGCT GCATGAGGAA CGTGAAGATC AGTAAAGCTG GAAAAACTCT GCAGGTCCAG 
ATGAACAAAC TGCTGGAGCT CAGAGGAGTT CAGCCGCTCA GCTGCCCTGC TGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A novel early onset phenotype in a zebrafish model of merosin deficient congenital muscular dystrophy.
PloS one12(2)e0172648(2017)
Smith SJ,Wang JC,Gupta VA,Dowling JJ

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Laminin and Matrix metalloproteinase 11 regulate Fibronectin levels in the zebrafish myotendinous junction.
Skeletal muscle618(2016)
Jenkins MH,Alrowaished SS,Goody MF,Crawford BD,Henry CA

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lama2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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