rfx1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	rfx1a
Entrez Gene ID	100038773
Full Name	regulatory factor X, 1a (influences HLA class II expression)
Synonyms	zgc:162914
General protein information	Preferred Names regulatory factor X, 1a (influences HLA class II expression) Names MHC class II regulatory factor RFX1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005163851.3	XP_005163908.1	MHC class II regulatory factor RFX1 isoform X2
XM_021468997.1	XP_021324672.1	MHC class II regulatory factor RFX1 isoform X1
XM_009299321.2	XP_009297596.1	MHC class II regulatory factor RFX1 isoform X1
XM_021469000.1	XP_021324675.1	MHC class II regulatory factor RFX1 isoform X2
XM_005163851.4	XP_005163908.1	MHC class II regulatory factor RFX1 isoform X3
NM_001089553.1	NP_001083022.1	MHC class II regulatory factor RFX1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Rfx1	NP_033081.3
Rattus norvegicus (Norway rat)	Rfx1	NP_001099414.1
Pan troglodytes (chimpanzee)	RFX1	XP_524133.2
Canis lupus familiaris (dog)	RFX1	XP_005632859.1
Xenopus tropicalis (tropical clawed frog)	rfx1	XP_002940666.2
Homo sapiens (human)	RFX1	NP_002909.4
Danio rerio (zebrafish)	rfx1a	NP_001083022.1

Gene Ontology
Bibliography

Related articles in PubMed

Function-driven discovery of disease genes in zebrafish using an integrated genomics big data resource.
Shim H, Kim JH, Kim CY, Hwang S, Kim H, Yang S, Lee JE, Lee I
Nucleic acids research44(20)9611-9623(2016 Nov)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Mutation of kri1l causes definitive hematopoiesis failure via PERK-dependent excessive autophagy induction.
Jia XE, Ma K, Xu T, Gao L, Wu S, Fu C, Zhang W, Wang Z, Liu K, Dong M, Jing C, Ren C, Dong Z, Chen Y, Jin Y, Huang Q, Chang X, Deng M, Li L, Luo L, Zhu J, Dang Y, Chang HC, Zon LI, Zhou Y, Chen S, Pan W
Cell research25(8)946-62(2015 Aug)

Rfx6 is an Ngn3-dependent winged helix transcription factor required for pancreatic islet cell development.
Soyer J, Flasse L, Raffelsberger W, Beucher A, Orvain C, Peers B, Ravassard P, Vermot J, Voz ML, Mellitzer G, Gradwohl G
Development (Cambridge, England)137(2)203-12(2010 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following rfx1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rfx1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa34618	NM_001089553.1 Latest version!	Danio rerio regulatory factor X, 1a (influences HLA class II expression) (rfx1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa08139	XM_005163851.4 Latest version!	Danio rerio regulatory factor X, 1a (influences HLA class II expression) (rfx1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa48429	XM_009299321.2 Latest version!	Danio rerio regulatory factor X, 1a (influences HLA class II expression) (rfx1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa141533	XM_021468997.1 Latest version!	Danio rerio regulatory factor X, 1a (influences HLA class II expression) (rfx1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
ODa141534	XM_021469000.1 Latest version!	Danio rerio regulatory factor X, 1a (influences HLA class II expression) (rfx1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
ODa08139	XM_005163851.3	Danio rerio regulatory factor X, 1a (influences HLA class II expression) (rfx1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rfx1a ( NM_001089553.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001083022.1 Danio rerio regulatory factor X, 1a (influences HLA class II expression) (rfx1a), mRNA.

Price & Availability↑

CloneID	ODa34618
Clone ID Related Accession (Same CDS sequence)	NM_001089553.1
Accession Version	NM_001089553.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	MHC class II regulatory factor RFX1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC139664.1. ##Evidence-Data-START## Transcript exon combination :: BC139664.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCAACTC CAGCATACGT GAGGGAGCTG CAGCAGACCT CCCAGCCTCA GGGGGGCAGT 
GTCACTCCTA CATCAGGACA GGCCTCTACA GTGACGGCAG GTCCTCAGCA ATTCCTGGCT 
GAACTTCAGA CTACAGGAGC CACGCCCTCA GCAGCCCCGC CAACTGGTCG GACCACACCT 
CCCACCTCAC AAGCACAGAA GAGCCAGGCG ATTGCACAGG CTCCACCCAC ACAAGCCCCG 
CCCACTCAAA CTCAGTACGT CACGGCTGAG ATTCAGAGCT CACCTACACA GTCCAGCAAT 
GCTCAGAATA CACCACAGTA CATTGTTGTC ACAGTTACAG AGGGTTCTCT ACACTCTAGT 
GACTCTGTGT CAGATTCCAG TCCTCCTGCT GCTGTGGTCC AGACTGGAGT TCCCACACAG 
GTGGTGCAGC AAGTACAGTC AGCCCAACAG AGGTCAGTAG TTCAAGCCAC ATCTCAGACA 
ACAAAAACAG AGCCGGGCAC GCAGCTGAAT GTCACCAGCC TACAGTCCGT GCACCTGACA 
CAGGAGGTAC AACAGCAGCT CCAGCAGGTG CCTGTCCAGC ATGTGTACAC TAACCAGGTG 
CAGTATGTGG AAGGAGGAGA AGCAAACTAC ACCACCAGCA CCATTCGTTC CAGCAGTTTC 
CCCTACACAG AGACCCCTCT GTACACCCAG AGCAGTGCGG GACAGTACTA CGAGGCCACC 
CCTGCCTCTG GATCTGAAGC CGCATCCCCA GGCACGCCTC TCACCGTCTC TGTCACCTCA 
GGATCTCCGG GTGTGCCCAT GTTTGTGGCA GGGACGGGGC AGATTGTGGC CAACCCCACC 
ACAGCGGCAG GCAGCGGGGT CACTGCAGTA GTAGCAGCTG CGGGGACGGC AGCTAACGGC 
TCTGGAGAAT CTGCCGGAGA GCCTAACGGT AGTGGCGGCA GCTATGTGAT CCAAGGTGGC 
TACATGTTGG CCGGCACCGG GACCGGTAGC AGTGGAAATG GTCACGGGTA CCCGCACCCC 
ACTCGCGCTT CTCCAGCCAC CCTGTCCTCT GGCCCTCAGG TGCAGTGGTT GTTGGATAAT 
TATGAGACAG CAGAAGGAGT GAGTTTGCCC AGGTCTACAC TTTACTGCCA CTACCTGCTG 
CACTGTCAGG AGCAGAAGCT GGAGCCAGTC AATGCTGCAT CCTTCGGGAA ACTCATCCGT 
TCTGTGTTCA TGGGCTTGAG GACCAGACGT CTGGGCACAC GAGGGAATTC CAAATGTCAC 
TACTATGGCC TGCGGATCAA AGCGAGCTCC TCTCTGCTCC GGCTGATGGA GGACCAGCAG 
CACCTGGCCA TGAGACAGCA GCCCTTCTCC CAGAAACAGA GGTTGAAGCC AGTTCAGAAG 
GTGGAGGGAA TCACCAACGG GATGTCGTCA GGATCTGGAC AGCAGCAGCA GCAGCAGCAG 
AGCTCGGGTT TGTCGGACAT CAGCACACAG GTCCAGCAGT ACCAGCAGTT TCTCGATGCG 
TCCAAAGCTC TCCCAGAGTT TCCTGATGTT GACCTGCAGG GTAAAGCTTT ACCAGAGGGC 
ATTGAGCTGG AACATCTCAA GAGTTTTCAG CTGCTCTACA GAGAACACTG CGAAGCCATT 
CTGGACGTGA TGGTCAACCT TCAGTTCCCT TTGGTGGAAA CTCTTTGGAA AACGTTTTGG 
AGGTTCAGTG AAAATCAGGC AGGAGACTCG GCAACACTAG CTGTGCACGA TGAGTCGGAG 
AAGAGGCTAC CCAAGACATG CTTGGTGATT CTCTGTAAAT ATGAGCCGGT GCTTCGCTGG 
AGTCGTGACT GCGACAACAC ACTGTACCAA GGCCTGGTGG AGATGCTCAT CCCAGACGTT 
CTGAGACCTA TTCCCAGTGC CTTAACACAA GCCATCCGAA ACTTTGCCAA GAGTCTTGAG 
AGCTGGTTGA CTAATGCCAT GGTGAACATT CCTGAAGAGA TGGTTCGTGT CAAGGTGACA 
TCTGCAAGTG CGTTTGCTCA GACTCTGCGC AGATACACCT CTCTAAACCA CCTGGCCCAG 
GCAGCTCGAG CCGTCCTGCA AAACACTGCG CAGATCAACC AGATGCTCAG CGACCTCAAC 
AGAGTGGATT TTGCAAACGT GCAGGAACAG GCCTCATGGG TGTGCCGGTG TGAGGACCGG 
GTGGTGCAGC GGCTTGAGCA GGACTTTAAG CTGACCCTTC AGCAGCAGAA CTCTCTGGAG 
CAGTGGGCCG CCTGGCTGGA TGGGGTGGTC TCGCAGGTCC TAAAGCCCTA CCAGTCCAGC 
GCTGCTCTTC CAAAAGCAGC TAAACTCTTC TTGCTGAAGT GGAGCTTTTA CAGCTCTATG 
GTGATCCGAG ATCTGACTCT CCGCAGTGCT GCCAGCTTCG GCTCTTTTCA CCTAATCCGT 
CTGCTCTACG ATGAGTACAT GTACTATCTG ATAGAGCACA GGGTTGCCCA GGCCAAGGGT 
GAAACACCGA TTGCTGTCAT GGGAGAGTTT GCAAGTCTTG GCCGAAGTCT GAACCCACTT 
GATCCTGATA AAGAAGAGGA AGAGGAGGAG GAAGAAGAGA GTGATGATGA GTGTCAGGAG 
TTATCTCTGC AGTCAGACGT GGGTGCGATG GGGGACGAAT CTCTGGAACC GCCTGCTAAA 
CTGGCCCGAA CCGACCAGAG GGTGTTGTTC ACCACAGGAA GCATGGAAAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001083022.1
CDS	70..2763
Translation	MATPAYVRELQQTSQPQGGSVTPTSGQASTVTAGPQQFLAELQTTGATPSAAPPTGRTTPPTSQAQKSQAIAQAPPTQAPPTQTQYVTAEIQSSPTQSSNAQNTPQYIVVTVTEGSLHSSDSVSDSSPPAAVVQTGVPTQVVQQVQSAQQRSVVQATSQTTKTEPGTQLNVTSLQSVHLTQEVQQQLQQVPVQHVYTNQVQYVEGGEANYTTSTIRSSSFPYTETPLYTQSSAGQYYEATPASGSEAASPGTPLTVSVTSGSPGVPMFVAGTGQIVANPTTAAGSGVTAVVAAAGTAANGSGESAGEPNGSGGSYVIQGGYMLAGTGTGSSGNGHGYPHPTRASPATLSSGPQVQWLLDNYETAEGVSLPRSTLYCHYLLHCQEQKLEPVNAASFGKLIRSVFMGLRTRRLGTRGNSKCHYYGLRIKASSSLLRLMEDQQHLAMRQQPFSQKQRLKPVQKVEGITNGMSSGSGQQQQQQQSSGLSDISTQVQQYQQFLDASKALPEFPDVDLQGKALPEGIELEHLKSFQLLYREHCEAILDVMVNLQFPLVETLWKTFWRFSENQAGDSATLAVHDESEKRLPKTCLVILCKYEPVLRWSRDCDNTLYQGLVEMLIPDVLRPIPSALTQAIRNFAKSLESWLTNAMVNIPEEMVRVKVTSASAFAQTLRRYTSLNHLAQAARAVLQNTAQINQMLSDLNRVDFANVQEQASWVCRCEDRVVQRLEQDFKLTLQQQNSLEQWAAWLDGVVSQVLKPYQSSAALPKAAKLFLLKWSFYSSMVIRDLTLRSAASFGSFHLIRLLYDEYMYYLIEHRVAQAKGETPIAVMGEFASLGRSLNPLDPDKEEEEEEEEESDDECQELSLQSDVGAMGDESLEPPAKLARTDQRVLFTTGSMEN

Target ORF information:

RefSeq Version	NM_001089553.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio regulatory factor X, 1a (influences HLA class II expression) (rfx1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001089553.1

ORF Insert Sequence:

ATGGCAACTC CAGCATACGT GAGGGAGCTG CAGCAGACCT CCCAGCCTCA GGGGGGCAGT 
GTCACTCCTA CATCAGGACA GGCCTCTACA GTGACGGCAG GTCCTCAGCA ATTCCTGGCT 
GAACTTCAGA CTACAGGAGC CACGCCCTCA GCAGCCCCGC CAACTGGTCG GACCACACCT 
CCCACCTCAC AAGCACAGAA GAGCCAGGCG ATTGCACAGG CTCCACCCAC ACAAGCCCCG 
CCCACTCAAA CTCAGTACGT CACGGCTGAG ATTCAGAGCT CACCTACACA GTCCAGCAAT 
GCTCAGAATA CACCACAGTA CATTGTTGTC ACAGTTACAG AGGGTTCTCT ACACTCTAGT 
GACTCTGTGT CAGATTCCAG TCCTCCTGCT GCTGTGGTCC AGACTGGAGT TCCCACACAG 
GTGGTGCAGC AAGTACAGTC AGCCCAACAG AGGTCAGTAG TTCAAGCCAC ATCTCAGACA 
ACAAAAACAG AGCCGGGCAC GCAGCTGAAT GTCACCAGCC TACAGTCCGT GCACCTGACA 
CAGGAGGTAC AACAGCAGCT CCAGCAGGTG CCTGTCCAGC ATGTGTACAC TAACCAGGTG 
CAGTATGTGG AAGGAGGAGA AGCAAACTAC ACCACCAGCA CCATTCGTTC CAGCAGTTTC 
CCCTACACAG AGACCCCTCT GTACACCCAG AGCAGTGCGG GACAGTACTA CGAGGCCACC 
CCTGCCTCTG GATCTGAAGC CGCATCCCCA GGCACGCCTC TCACCGTCTC TGTCACCTCA 
GGATCTCCGG GTGTGCCCAT GTTTGTGGCA GGGACGGGGC AGATTGTGGC CAACCCCACC 
ACAGCGGCAG GCAGCGGGGT CACTGCAGTA GTAGCAGCTG CGGGGACGGC AGCTAACGGC 
TCTGGAGAAT CTGCCGGAGA GCCTAACGGT AGTGGCGGCA GCTATGTGAT CCAAGGTGGC 
TACATGTTGG CCGGCACCGG GACCGGTAGC AGTGGAAATG GTCACGGGTA CCCGCACCCC 
ACTCGCGCTT CTCCAGCCAC CCTGTCCTCT GGCCCTCAGG TGCAGTGGTT GTTGGATAAT 
TATGAGACAG CAGAAGGAGT GAGTTTGCCC AGGTCTACAC TTTACTGCCA CTACCTGCTG 
CACTGTCAGG AGCAGAAGCT GGAGCCAGTC AATGCTGCAT CCTTCGGGAA ACTCATCCGT 
TCTGTGTTCA TGGGCTTGAG GACCAGACGT CTGGGCACAC GAGGGAATTC CAAATGTCAC 
TACTATGGCC TGCGGATCAA AGCGAGCTCC TCTCTGCTCC GGCTGATGGA GGACCAGCAG 
CACCTGGCCA TGAGACAGCA GCCCTTCTCC CAGAAACAGA GGTTGAAGCC AGTTCAGAAG 
GTGGAGGGAA TCACCAACGG GATGTCGTCA GGATCTGGAC AGCAGCAGCA GCAGCAGCAG 
AGCTCGGGTT TGTCGGACAT CAGCACACAG GTCCAGCAGT ACCAGCAGTT TCTCGATGCG 
TCCAAAGCTC TCCCAGAGTT TCCTGATGTT GACCTGCAGG GTAAAGCTTT ACCAGAGGGC 
ATTGAGCTGG AACATCTCAA GAGTTTTCAG CTGCTCTACA GAGAACACTG CGAAGCCATT 
CTGGACGTGA TGGTCAACCT TCAGTTCCCT TTGGTGGAAA CTCTTTGGAA AACGTTTTGG 
AGGTTCAGTG AAAATCAGGC AGGAGACTCG GCAACACTAG CTGTGCACGA TGAGTCGGAG 
AAGAGGCTAC CCAAGACATG CTTGGTGATT CTCTGTAAAT ATGAGCCGGT GCTTCGCTGG 
AGTCGTGACT GCGACAACAC ACTGTACCAA GGCCTGGTGG AGATGCTCAT CCCAGACGTT 
CTGAGACCTA TTCCCAGTGC CTTAACACAA GCCATCCGAA ACTTTGCCAA GAGTCTTGAG 
AGCTGGTTGA CTAATGCCAT GGTGAACATT CCTGAAGAGA TGGTTCGTGT CAAGGTGACA 
TCTGCAAGTG CGTTTGCTCA GACTCTGCGC AGATACACCT CTCTAAACCA CCTGGCCCAG 
GCAGCTCGAG CCGTCCTGCA AAACACTGCG CAGATCAACC AGATGCTCAG CGACCTCAAC 
AGAGTGGATT TTGCAAACGT GCAGGAACAG GCCTCATGGG TGTGCCGGTG TGAGGACCGG 
GTGGTGCAGC GGCTTGAGCA GGACTTTAAG CTGACCCTTC AGCAGCAGAA CTCTCTGGAG 
CAGTGGGCCG CCTGGCTGGA TGGGGTGGTC TCGCAGGTCC TAAAGCCCTA CCAGTCCAGC 
GCTGCTCTTC CAAAAGCAGC TAAACTCTTC TTGCTGAAGT GGAGCTTTTA CAGCTCTATG 
GTGATCCGAG ATCTGACTCT CCGCAGTGCT GCCAGCTTCG GCTCTTTTCA CCTAATCCGT 
CTGCTCTACG ATGAGTACAT GTACTATCTG ATAGAGCACA GGGTTGCCCA GGCCAAGGGT 
GAAACACCGA TTGCTGTCAT GGGAGAGTTT GCAAGTCTTG GCCGAAGTCT GAACCCACTT 
GATCCTGATA AAGAAGAGGA AGAGGAGGAG GAAGAAGAGA GTGATGATGA GTGTCAGGAG 
TTATCTCTGC AGTCAGACGT GGGTGCGATG GGGGACGAAT CTCTGGAACC GCCTGCTAAA 
CTGGCCCGAA CCGACCAGAG GGTGTTGTTC ACCACAGGAA GCATGGAAAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rfx1a ( XM_005163851.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005163908.1 Danio rerio regulatory factor X, 1a (influences HLA class II expression) (rfx1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa08139
Clone ID Related Accession (Same CDS sequence)	XM_005163851.3 , XM_005163851.4
Accession Version	XM_005163851.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	MHC class II regulatory factor RFX1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005163851.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACTC CAGCATACGT GAGGGAGCTG CAGCAGACCT CCCAGCCTCA GGGGGGCAGT 
GTCACTCCTA CATCAGGACA GGCCTCTACT GTGACGGCGG GTCCTCAGCA ATTCCTGGCT 
GAACTTCAGA CTACAGGAGC CACGCCCTCA GCAGCCCCGC CAACTGGTCG GACCACACCT 
CCCACCTCAC AAGCACAGAA GAGCCAGGCG ATTGCACAGG CTCCACCCAC ACAAGCCCCG 
CCCACTCAAA CTCAGTACGT CACGGCTGAG ATTCAGAGCT CACCTACACA GTCCAGCAAT 
GCTCAGAATA CACCGCAGTA CATTGTTGTC ACAGTTACAG AGGGTTCTCT ACACTCTAGT 
GACTCTGTGT CAGATTCCAG TCCTCCTGCT GCTGTGGTCC AGACTGGAGT TCCCACACAG 
GTGGTGCAGC AAGTACAGTC AGCCCAACAG AGGTCAGTAG TTCAAGCCAC ATCTCAGACA 
ACAAAAACAG AGCCGGGCAC GCAGCTGAAT GTCACCAGCC TACAGTCCGT GCACCTGACA 
CAGGAGGTAC AGCAGCAGCT CCAGCAGGTG CCTGTCCAGC ATGTGTACAC TAACCAGGTG 
CAGTATGTGG AAGGAGGAGA AGCAAACTAC ACCACCAGCA CCATTCGTTC CAGCAGTTTC 
CCCTACACAG AGACCCCTCT GTACACCCAG AGCAGTGCGG GACAGTACTA CGAGGCCACC 
CCTGCCTCTG GATCTGAAGC CGCATCCCCC GGCACGCCTC TCACCGTCTC TGTCACCTCA 
GGATCTCCAG GTGTGCCCAT GTTTGTGGCA GGGACGGGGC AGATTGTGGC CAACCCCACC 
ACAGCGGCAG GCAGTGGGGT CACTGCAGTA GTAGCAGCTG CGGGGACGGC AGCTAACGGC 
TCTGGAGAAT CTGCCGGAGA GGCTAACGGT AGTGGCGGCA GCTATGTGAT CCAAGGTGGC 
TACATGTTGG CCGGCACCGG GAGTGGTAGC AGTGGAAACG GTCACGGGTA CCCGCACCCC 
ACTCGCGCTT CTCCAGCCAC CGTGCAGTGG TTGTTGGATA ATTATGAGAC AGCAGAAGGA 
GTGAGTTTGC CCAGGTCTAC ACTTTACTGC CACTACCTGC TGCACTGTCA GGAGCAGAAG 
CTGGAGCCAG TTAATGCTGC ATCCTTTGGG AAACTGATCC GTTCTGTGTT CATGGGCTTG 
AGGACCAGAC GTCTGGGCAC ACGAGGGAAT TCCAAATATC ACTACTATGG CCTGCGGATC 
AAAGCGAGCT CCTCTCTGCT CCGGCTGATG GAGGACCAGC AGCACCTGGC CATGAGACAG 
CAGCCCTTCT CCCAGAAACA GAGGTTGAAG CCAGTTCAGA AGGTGGAGGG AATCACCAAC 
GGGATGTCGT CAGGATCTGG ACAGCAGCAG CAGCAGCAGC AGAGCTCGGG TTTGTCGGAC 
ATCAGCACAC AGGTCCAGCA GTACCAGCAG TTTCTCGATG CGTCCAAAGC TCTCCCAGAG 
TTTCCTGATG TTGACCTGCA GGGTAAAGCT TTACCAGAGG GCATTGAGCT GGAACATCTC 
AAGAGTTTTC AGCTGCTCTA CAGAGAACAC TGCGAAGCCA TTCTGGACGT GATGGTCAAC 
CTTCAGTTCC CTTTGGTGGA AACTCTTTGG AAAACGTTTT GGAGGTTCAG TGAAAATCAG 
GCAGGAGACT CGGCAACACT AGCTGTGCAC GATGAGTCGG AGAAGAGGCT ACCCAAGACA 
TGCTTGGTGA TTCTCTGTAA ATATGAGCCG GTGCTTCGCT GGAGTCGTGA CTGCGACAAC 
ACACTGTACC AAGGCCTGGT GGAGATGCTC ATCCCAGACG TTCTGAGACC TATTCCCAGT 
GCCTTAACAC AAGCCATCCG AAACTTTGCC AAGAGTCTTG AGAGCTGGTT GACTAATGCC 
ATGGTGAACA TTCCTGAAGA GATGGTTCGT GTCAAGGTGA CATCTGCAAG TGCGTTTGCT 
CAGACTCTGC GCAGATACAC CTCTCTAAAC CACCTGGCTC AGGCAGCTCG AGCCGTCCTG 
CAAAACACTG CGCAGATCAA CCAGATGCTC AGCGACCTCA ACAGAGTGGA TTTTGCAAAT 
GTGCAGGAAC AGGCCTCATG GGTGTGCCGG TGTGAGGACC GGGTGGTGCA GCGGCTTGAG 
CAGGACTTTA AGCTGACCCT TCAGCAGCAG AACTCTCTGG AGCAGTGGGC CGCCTGGCTG 
GATGGGGTGG TCTCGCAGGT CCTAAAGCCC TACCAGTCCA GCGCTGCTCT TCCAAAAGCA 
GCTAAACTCT TCTTGCTGAA GTGGAGCTTT TACAGCTCTA TGGTGATCCG AGATCTGACT 
CTCCGCAGTG CTGCCAGCTT CGGCTCTTTT CACCTAATCC GTCTGCTCTA CGATGAGTAC 
ATGTACTATC TGATAGAGCA CAGGGTTGCC CAGGCCAAGG GTGAAACACC GATTGCTGTC 
ATGGGAGAGT TTGCAAGTCT TGGCCGAAGT CTGAACCCAC TTGATCCTGA TAAAGAAGAG 
GAAGAGGAGG AGGAAGAAGA GAGTGATGAT GAGTGTCAGG AGTTATCTCT GCAGTCAGAC 
GTGGGTGCGA TGGGGGACGA ATCTCTGGAA CCGCCTGCTA AACTGGCCCG AACCGACCAG 
AGGGTGTTGT TCACCACAGG AAGCATGGAA AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163908.1
CDS	335..3010
Translation	MATPAYVRELQQTSQPQGGSVTPTSGQASTVTAGPQQFLAELQTTGATPSAAPPTGRTTPPTSQAQKSQAIAQAPPTQAPPTQTQYVTAEIQSSPTQSSNAQNTPQYIVVTVTEGSLHSSDSVSDSSPPAAVVQTGVPTQVVQQVQSAQQRSVVQATSQTTKTEPGTQLNVTSLQSVHLTQEVQQQLQQVPVQHVYTNQVQYVEGGEANYTTSTIRSSSFPYTETPLYTQSSAGQYYEATPASGSEAASPGTPLTVSVTSGSPGVPMFVAGTGQIVANPTTAAGSGVTAVVAAAGTAANGSGESAGEANGSGGSYVIQGGYMLAGTGSGSSGNGHGYPHPTRASPATVQWLLDNYETAEGVSLPRSTLYCHYLLHCQEQKLEPVNAASFGKLIRSVFMGLRTRRLGTRGNSKYHYYGLRIKASSSLLRLMEDQQHLAMRQQPFSQKQRLKPVQKVEGITNGMSSGSGQQQQQQQSSGLSDISTQVQQYQQFLDASKALPEFPDVDLQGKALPEGIELEHLKSFQLLYREHCEAILDVMVNLQFPLVETLWKTFWRFSENQAGDSATLAVHDESEKRLPKTCLVILCKYEPVLRWSRDCDNTLYQGLVEMLIPDVLRPIPSALTQAIRNFAKSLESWLTNAMVNIPEEMVRVKVTSASAFAQTLRRYTSLNHLAQAARAVLQNTAQINQMLSDLNRVDFANVQEQASWVCRCEDRVVQRLEQDFKLTLQQQNSLEQWAAWLDGVVSQVLKPYQSSAALPKAAKLFLLKWSFYSSMVIRDLTLRSAASFGSFHLIRLLYDEYMYYLIEHRVAQAKGETPIAVMGEFASLGRSLNPLDPDKEEEEEEEEESDDECQELSLQSDVGAMGDESLEPPAKLARTDQRVLFTTGSMEN

Target ORF information:

RefSeq Version	XM_005163851.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio regulatory factor X, 1a (influences HLA class II expression) (rfx1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163851.3

ORF Insert Sequence:

ATGGCAACTC CAGCATACGT GAGGGAGCTG CAGCAGACCT CCCAGCCTCA GGGGGGCAGT 
GTCACTCCTA CATCAGGACA GGCCTCTACT GTGACGGCGG GTCCTCAGCA ATTCCTGGCT 
GAACTTCAGA CTACAGGAGC CACGCCCTCA GCAGCCCCGC CAACTGGTCG GACCACACCT 
CCCACCTCAC AAGCACAGAA GAGCCAGGCG ATTGCACAGG CTCCACCCAC ACAAGCCCCG 
CCCACTCAAA CTCAGTACGT CACGGCTGAG ATTCAGAGCT CACCTACACA GTCCAGCAAT 
GCTCAGAATA CACCGCAGTA CATTGTTGTC ACAGTTACAG AGGGTTCTCT ACACTCTAGT 
GACTCTGTGT CAGATTCCAG TCCTCCTGCT GCTGTGGTCC AGACTGGAGT TCCCACACAG 
GTGGTGCAGC AAGTACAGTC AGCCCAACAG AGGTCAGTAG TTCAAGCCAC ATCTCAGACA 
ACAAAAACAG AGCCGGGCAC GCAGCTGAAT GTCACCAGCC TACAGTCCGT GCACCTGACA 
CAGGAGGTAC AGCAGCAGCT CCAGCAGGTG CCTGTCCAGC ATGTGTACAC TAACCAGGTG 
CAGTATGTGG AAGGAGGAGA AGCAAACTAC ACCACCAGCA CCATTCGTTC CAGCAGTTTC 
CCCTACACAG AGACCCCTCT GTACACCCAG AGCAGTGCGG GACAGTACTA CGAGGCCACC 
CCTGCCTCTG GATCTGAAGC CGCATCCCCC GGCACGCCTC TCACCGTCTC TGTCACCTCA 
GGATCTCCAG GTGTGCCCAT GTTTGTGGCA GGGACGGGGC AGATTGTGGC CAACCCCACC 
ACAGCGGCAG GCAGTGGGGT CACTGCAGTA GTAGCAGCTG CGGGGACGGC AGCTAACGGC 
TCTGGAGAAT CTGCCGGAGA GGCTAACGGT AGTGGCGGCA GCTATGTGAT CCAAGGTGGC 
TACATGTTGG CCGGCACCGG GAGTGGTAGC AGTGGAAACG GTCACGGGTA CCCGCACCCC 
ACTCGCGCTT CTCCAGCCAC CGTGCAGTGG TTGTTGGATA ATTATGAGAC AGCAGAAGGA 
GTGAGTTTGC CCAGGTCTAC ACTTTACTGC CACTACCTGC TGCACTGTCA GGAGCAGAAG 
CTGGAGCCAG TTAATGCTGC ATCCTTTGGG AAACTGATCC GTTCTGTGTT CATGGGCTTG 
AGGACCAGAC GTCTGGGCAC ACGAGGGAAT TCCAAATATC ACTACTATGG CCTGCGGATC 
AAAGCGAGCT CCTCTCTGCT CCGGCTGATG GAGGACCAGC AGCACCTGGC CATGAGACAG 
CAGCCCTTCT CCCAGAAACA GAGGTTGAAG CCAGTTCAGA AGGTGGAGGG AATCACCAAC 
GGGATGTCGT CAGGATCTGG ACAGCAGCAG CAGCAGCAGC AGAGCTCGGG TTTGTCGGAC 
ATCAGCACAC AGGTCCAGCA GTACCAGCAG TTTCTCGATG CGTCCAAAGC TCTCCCAGAG 
TTTCCTGATG TTGACCTGCA GGGTAAAGCT TTACCAGAGG GCATTGAGCT GGAACATCTC 
AAGAGTTTTC AGCTGCTCTA CAGAGAACAC TGCGAAGCCA TTCTGGACGT GATGGTCAAC 
CTTCAGTTCC CTTTGGTGGA AACTCTTTGG AAAACGTTTT GGAGGTTCAG TGAAAATCAG 
GCAGGAGACT CGGCAACACT AGCTGTGCAC GATGAGTCGG AGAAGAGGCT ACCCAAGACA 
TGCTTGGTGA TTCTCTGTAA ATATGAGCCG GTGCTTCGCT GGAGTCGTGA CTGCGACAAC 
ACACTGTACC AAGGCCTGGT GGAGATGCTC ATCCCAGACG TTCTGAGACC TATTCCCAGT 
GCCTTAACAC AAGCCATCCG AAACTTTGCC AAGAGTCTTG AGAGCTGGTT GACTAATGCC 
ATGGTGAACA TTCCTGAAGA GATGGTTCGT GTCAAGGTGA CATCTGCAAG TGCGTTTGCT 
CAGACTCTGC GCAGATACAC CTCTCTAAAC CACCTGGCTC AGGCAGCTCG AGCCGTCCTG 
CAAAACACTG CGCAGATCAA CCAGATGCTC AGCGACCTCA ACAGAGTGGA TTTTGCAAAT 
GTGCAGGAAC AGGCCTCATG GGTGTGCCGG TGTGAGGACC GGGTGGTGCA GCGGCTTGAG 
CAGGACTTTA AGCTGACCCT TCAGCAGCAG AACTCTCTGG AGCAGTGGGC CGCCTGGCTG 
GATGGGGTGG TCTCGCAGGT CCTAAAGCCC TACCAGTCCA GCGCTGCTCT TCCAAAAGCA 
GCTAAACTCT TCTTGCTGAA GTGGAGCTTT TACAGCTCTA TGGTGATCCG AGATCTGACT 
CTCCGCAGTG CTGCCAGCTT CGGCTCTTTT CACCTAATCC GTCTGCTCTA CGATGAGTAC 
ATGTACTATC TGATAGAGCA CAGGGTTGCC CAGGCCAAGG GTGAAACACC GATTGCTGTC 
ATGGGAGAGT TTGCAAGTCT TGGCCGAAGT CTGAACCCAC TTGATCCTGA TAAAGAAGAG 
GAAGAGGAGG AGGAAGAAGA GAGTGATGAT GAGTGTCAGG AGTTATCTCT GCAGTCAGAC 
GTGGGTGCGA TGGGGGACGA ATCTCTGGAA CCGCCTGCTA AACTGGCCCG AACCGACCAG 
AGGGTGTTGT TCACCACAGG AAGCATGGAA AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rfx1a ( XM_005163851.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005163908.1 Danio rerio regulatory factor X, 1a (influences HLA class II expression) (rfx1a), transcript variant X3, mRNA.

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CloneID	ODa08139
Clone ID Related Accession (Same CDS sequence)	XM_005163851.3 , XM_005163851.4
Accession Version	XM_005163851.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	MHC class II regulatory factor RFX1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005163851.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACTC CAGCATACGT GAGGGAGCTG CAGCAGACCT CCCAGCCTCA GGGGGGCAGT 
GTCACTCCTA CATCAGGACA GGCCTCTACT GTGACGGCGG GTCCTCAGCA ATTCCTGGCT 
GAACTTCAGA CTACAGGAGC CACGCCCTCA GCAGCCCCGC CAACTGGTCG GACCACACCT 
CCCACCTCAC AAGCACAGAA GAGCCAGGCG ATTGCACAGG CTCCACCCAC ACAAGCCCCG 
CCCACTCAAA CTCAGTACGT CACGGCTGAG ATTCAGAGCT CACCTACACA GTCCAGCAAT 
GCTCAGAATA CACCGCAGTA CATTGTTGTC ACAGTTACAG AGGGTTCTCT ACACTCTAGT 
GACTCTGTGT CAGATTCCAG TCCTCCTGCT GCTGTGGTCC AGACTGGAGT TCCCACACAG 
GTGGTGCAGC AAGTACAGTC AGCCCAACAG AGGTCAGTAG TTCAAGCCAC ATCTCAGACA 
ACAAAAACAG AGCCGGGCAC GCAGCTGAAT GTCACCAGCC TACAGTCCGT GCACCTGACA 
CAGGAGGTAC AGCAGCAGCT CCAGCAGGTG CCTGTCCAGC ATGTGTACAC TAACCAGGTG 
CAGTATGTGG AAGGAGGAGA AGCAAACTAC ACCACCAGCA CCATTCGTTC CAGCAGTTTC 
CCCTACACAG AGACCCCTCT GTACACCCAG AGCAGTGCGG GACAGTACTA CGAGGCCACC 
CCTGCCTCTG GATCTGAAGC CGCATCCCCC GGCACGCCTC TCACCGTCTC TGTCACCTCA 
GGATCTCCAG GTGTGCCCAT GTTTGTGGCA GGGACGGGGC AGATTGTGGC CAACCCCACC 
ACAGCGGCAG GCAGTGGGGT CACTGCAGTA GTAGCAGCTG CGGGGACGGC AGCTAACGGC 
TCTGGAGAAT CTGCCGGAGA GGCTAACGGT AGTGGCGGCA GCTATGTGAT CCAAGGTGGC 
TACATGTTGG CCGGCACCGG GAGTGGTAGC AGTGGAAACG GTCACGGGTA CCCGCACCCC 
ACTCGCGCTT CTCCAGCCAC CGTGCAGTGG TTGTTGGATA ATTATGAGAC AGCAGAAGGA 
GTGAGTTTGC CCAGGTCTAC ACTTTACTGC CACTACCTGC TGCACTGTCA GGAGCAGAAG 
CTGGAGCCAG TTAATGCTGC ATCCTTTGGG AAACTGATCC GTTCTGTGTT CATGGGCTTG 
AGGACCAGAC GTCTGGGCAC ACGAGGGAAT TCCAAATATC ACTACTATGG CCTGCGGATC 
AAAGCGAGCT CCTCTCTGCT CCGGCTGATG GAGGACCAGC AGCACCTGGC CATGAGACAG 
CAGCCCTTCT CCCAGAAACA GAGGTTGAAG CCAGTTCAGA AGGTGGAGGG AATCACCAAC 
GGGATGTCGT CAGGATCTGG ACAGCAGCAG CAGCAGCAGC AGAGCTCGGG TTTGTCGGAC 
ATCAGCACAC AGGTCCAGCA GTACCAGCAG TTTCTCGATG CGTCCAAAGC TCTCCCAGAG 
TTTCCTGATG TTGACCTGCA GGGTAAAGCT TTACCAGAGG GCATTGAGCT GGAACATCTC 
AAGAGTTTTC AGCTGCTCTA CAGAGAACAC TGCGAAGCCA TTCTGGACGT GATGGTCAAC 
CTTCAGTTCC CTTTGGTGGA AACTCTTTGG AAAACGTTTT GGAGGTTCAG TGAAAATCAG 
GCAGGAGACT CGGCAACACT AGCTGTGCAC GATGAGTCGG AGAAGAGGCT ACCCAAGACA 
TGCTTGGTGA TTCTCTGTAA ATATGAGCCG GTGCTTCGCT GGAGTCGTGA CTGCGACAAC 
ACACTGTACC AAGGCCTGGT GGAGATGCTC ATCCCAGACG TTCTGAGACC TATTCCCAGT 
GCCTTAACAC AAGCCATCCG AAACTTTGCC AAGAGTCTTG AGAGCTGGTT GACTAATGCC 
ATGGTGAACA TTCCTGAAGA GATGGTTCGT GTCAAGGTGA CATCTGCAAG TGCGTTTGCT 
CAGACTCTGC GCAGATACAC CTCTCTAAAC CACCTGGCTC AGGCAGCTCG AGCCGTCCTG 
CAAAACACTG CGCAGATCAA CCAGATGCTC AGCGACCTCA ACAGAGTGGA TTTTGCAAAT 
GTGCAGGAAC AGGCCTCATG GGTGTGCCGG TGTGAGGACC GGGTGGTGCA GCGGCTTGAG 
CAGGACTTTA AGCTGACCCT TCAGCAGCAG AACTCTCTGG AGCAGTGGGC CGCCTGGCTG 
GATGGGGTGG TCTCGCAGGT CCTAAAGCCC TACCAGTCCA GCGCTGCTCT TCCAAAAGCA 
GCTAAACTCT TCTTGCTGAA GTGGAGCTTT TACAGCTCTA TGGTGATCCG AGATCTGACT 
CTCCGCAGTG CTGCCAGCTT CGGCTCTTTT CACCTAATCC GTCTGCTCTA CGATGAGTAC 
ATGTACTATC TGATAGAGCA CAGGGTTGCC CAGGCCAAGG GTGAAACACC GATTGCTGTC 
ATGGGAGAGT TTGCAAGTCT TGGCCGAAGT CTGAACCCAC TTGATCCTGA TAAAGAAGAG 
GAAGAGGAGG AGGAAGAAGA GAGTGATGAT GAGTGTCAGG AGTTATCTCT GCAGTCAGAC 
GTGGGTGCGA TGGGGGACGA ATCTCTGGAA CCGCCTGCTA AACTGGCCCG AACCGACCAG 
AGGGTGTTGT TCACCACAGG AAGCATGGAA AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163908.1
CDS	299..2974
Translation	MATPAYVRELQQTSQPQGGSVTPTSGQASTVTAGPQQFLAELQTTGATPSAAPPTGRTTPPTSQAQKSQAIAQAPPTQAPPTQTQYVTAEIQSSPTQSSNAQNTPQYIVVTVTEGSLHSSDSVSDSSPPAAVVQTGVPTQVVQQVQSAQQRSVVQATSQTTKTEPGTQLNVTSLQSVHLTQEVQQQLQQVPVQHVYTNQVQYVEGGEANYTTSTIRSSSFPYTETPLYTQSSAGQYYEATPASGSEAASPGTPLTVSVTSGSPGVPMFVAGTGQIVANPTTAAGSGVTAVVAAAGTAANGSGESAGEANGSGGSYVIQGGYMLAGTGSGSSGNGHGYPHPTRASPATVQWLLDNYETAEGVSLPRSTLYCHYLLHCQEQKLEPVNAASFGKLIRSVFMGLRTRRLGTRGNSKYHYYGLRIKASSSLLRLMEDQQHLAMRQQPFSQKQRLKPVQKVEGITNGMSSGSGQQQQQQQSSGLSDISTQVQQYQQFLDASKALPEFPDVDLQGKALPEGIELEHLKSFQLLYREHCEAILDVMVNLQFPLVETLWKTFWRFSENQAGDSATLAVHDESEKRLPKTCLVILCKYEPVLRWSRDCDNTLYQGLVEMLIPDVLRPIPSALTQAIRNFAKSLESWLTNAMVNIPEEMVRVKVTSASAFAQTLRRYTSLNHLAQAARAVLQNTAQINQMLSDLNRVDFANVQEQASWVCRCEDRVVQRLEQDFKLTLQQQNSLEQWAAWLDGVVSQVLKPYQSSAALPKAAKLFLLKWSFYSSMVIRDLTLRSAASFGSFHLIRLLYDEYMYYLIEHRVAQAKGETPIAVMGEFASLGRSLNPLDPDKEEEEEEEEESDDECQELSLQSDVGAMGDESLEPPAKLARTDQRVLFTTGSMEN

Target ORF information:

RefSeq Version	XM_005163851.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio regulatory factor X, 1a (influences HLA class II expression) (rfx1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163851.4

ORF Insert Sequence:

ATGGCAACTC CAGCATACGT GAGGGAGCTG CAGCAGACCT CCCAGCCTCA GGGGGGCAGT 
GTCACTCCTA CATCAGGACA GGCCTCTACT GTGACGGCGG GTCCTCAGCA ATTCCTGGCT 
GAACTTCAGA CTACAGGAGC CACGCCCTCA GCAGCCCCGC CAACTGGTCG GACCACACCT 
CCCACCTCAC AAGCACAGAA GAGCCAGGCG ATTGCACAGG CTCCACCCAC ACAAGCCCCG 
CCCACTCAAA CTCAGTACGT CACGGCTGAG ATTCAGAGCT CACCTACACA GTCCAGCAAT 
GCTCAGAATA CACCGCAGTA CATTGTTGTC ACAGTTACAG AGGGTTCTCT ACACTCTAGT 
GACTCTGTGT CAGATTCCAG TCCTCCTGCT GCTGTGGTCC AGACTGGAGT TCCCACACAG 
GTGGTGCAGC AAGTACAGTC AGCCCAACAG AGGTCAGTAG TTCAAGCCAC ATCTCAGACA 
ACAAAAACAG AGCCGGGCAC GCAGCTGAAT GTCACCAGCC TACAGTCCGT GCACCTGACA 
CAGGAGGTAC AGCAGCAGCT CCAGCAGGTG CCTGTCCAGC ATGTGTACAC TAACCAGGTG 
CAGTATGTGG AAGGAGGAGA AGCAAACTAC ACCACCAGCA CCATTCGTTC CAGCAGTTTC 
CCCTACACAG AGACCCCTCT GTACACCCAG AGCAGTGCGG GACAGTACTA CGAGGCCACC 
CCTGCCTCTG GATCTGAAGC CGCATCCCCC GGCACGCCTC TCACCGTCTC TGTCACCTCA 
GGATCTCCAG GTGTGCCCAT GTTTGTGGCA GGGACGGGGC AGATTGTGGC CAACCCCACC 
ACAGCGGCAG GCAGTGGGGT CACTGCAGTA GTAGCAGCTG CGGGGACGGC AGCTAACGGC 
TCTGGAGAAT CTGCCGGAGA GGCTAACGGT AGTGGCGGCA GCTATGTGAT CCAAGGTGGC 
TACATGTTGG CCGGCACCGG GAGTGGTAGC AGTGGAAACG GTCACGGGTA CCCGCACCCC 
ACTCGCGCTT CTCCAGCCAC CGTGCAGTGG TTGTTGGATA ATTATGAGAC AGCAGAAGGA 
GTGAGTTTGC CCAGGTCTAC ACTTTACTGC CACTACCTGC TGCACTGTCA GGAGCAGAAG 
CTGGAGCCAG TTAATGCTGC ATCCTTTGGG AAACTGATCC GTTCTGTGTT CATGGGCTTG 
AGGACCAGAC GTCTGGGCAC ACGAGGGAAT TCCAAATATC ACTACTATGG CCTGCGGATC 
AAAGCGAGCT CCTCTCTGCT CCGGCTGATG GAGGACCAGC AGCACCTGGC CATGAGACAG 
CAGCCCTTCT CCCAGAAACA GAGGTTGAAG CCAGTTCAGA AGGTGGAGGG AATCACCAAC 
GGGATGTCGT CAGGATCTGG ACAGCAGCAG CAGCAGCAGC AGAGCTCGGG TTTGTCGGAC 
ATCAGCACAC AGGTCCAGCA GTACCAGCAG TTTCTCGATG CGTCCAAAGC TCTCCCAGAG 
TTTCCTGATG TTGACCTGCA GGGTAAAGCT TTACCAGAGG GCATTGAGCT GGAACATCTC 
AAGAGTTTTC AGCTGCTCTA CAGAGAACAC TGCGAAGCCA TTCTGGACGT GATGGTCAAC 
CTTCAGTTCC CTTTGGTGGA AACTCTTTGG AAAACGTTTT GGAGGTTCAG TGAAAATCAG 
GCAGGAGACT CGGCAACACT AGCTGTGCAC GATGAGTCGG AGAAGAGGCT ACCCAAGACA 
TGCTTGGTGA TTCTCTGTAA ATATGAGCCG GTGCTTCGCT GGAGTCGTGA CTGCGACAAC 
ACACTGTACC AAGGCCTGGT GGAGATGCTC ATCCCAGACG TTCTGAGACC TATTCCCAGT 
GCCTTAACAC AAGCCATCCG AAACTTTGCC AAGAGTCTTG AGAGCTGGTT GACTAATGCC 
ATGGTGAACA TTCCTGAAGA GATGGTTCGT GTCAAGGTGA CATCTGCAAG TGCGTTTGCT 
CAGACTCTGC GCAGATACAC CTCTCTAAAC CACCTGGCTC AGGCAGCTCG AGCCGTCCTG 
CAAAACACTG CGCAGATCAA CCAGATGCTC AGCGACCTCA ACAGAGTGGA TTTTGCAAAT 
GTGCAGGAAC AGGCCTCATG GGTGTGCCGG TGTGAGGACC GGGTGGTGCA GCGGCTTGAG 
CAGGACTTTA AGCTGACCCT TCAGCAGCAG AACTCTCTGG AGCAGTGGGC CGCCTGGCTG 
GATGGGGTGG TCTCGCAGGT CCTAAAGCCC TACCAGTCCA GCGCTGCTCT TCCAAAAGCA 
GCTAAACTCT TCTTGCTGAA GTGGAGCTTT TACAGCTCTA TGGTGATCCG AGATCTGACT 
CTCCGCAGTG CTGCCAGCTT CGGCTCTTTT CACCTAATCC GTCTGCTCTA CGATGAGTAC 
ATGTACTATC TGATAGAGCA CAGGGTTGCC CAGGCCAAGG GTGAAACACC GATTGCTGTC 
ATGGGAGAGT TTGCAAGTCT TGGCCGAAGT CTGAACCCAC TTGATCCTGA TAAAGAAGAG 
GAAGAGGAGG AGGAAGAAGA GAGTGATGAT GAGTGTCAGG AGTTATCTCT GCAGTCAGAC 
GTGGGTGCGA TGGGGGACGA ATCTCTGGAA CCGCCTGCTA AACTGGCCCG AACCGACCAG 
AGGGTGTTGT TCACCACAGG AAGCATGGAA AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rfx1a ( XM_009299321.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009297596.1 Danio rerio regulatory factor X, 1a (influences HLA class II expression) (rfx1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa48429
Clone ID Related Accession (Same CDS sequence)	XM_009299321.2
Accession Version	XM_009299321.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	MHC class II regulatory factor RFX1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299321.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACTC CAGCATACGT GAGGGAGCTG CAGCAGACCT CCCAGCCTCA GGGGGGCAGT 
GTCACTCCTA CATCAGGACA GGCCTCTACT GTGACGGCGG GTCCTCAGCA ATTCCTGGCT 
GAACTTCAGA CTACAGGAGC CACGCCCTCA GCAGCCCCGC CAACTGGTCG GACCACACCT 
CCCACCTCAC AAGCACAGAA GAGCCAGGCG ATTGCACAGG CTCCACCCAC ACAAGCCCCG 
CCCACTCAAA CTCAGTACGT CACGGCTGAG ATTCAGAGCT CACCTACACA GTCCAGCAAT 
GCTCAGAATA CACCGCAGTA CATTGTTGTC ACAGTTACAG AGGGTTCTCT ACACTCTAGT 
GACTCTGTGT CAGATTCCAG TCCTCCTGCT GCTGTGGTCC AGACTGGAGT TCCCACACAG 
GTGGTGCAGC AAGTACAGTC AGCCCAACAG AGGTCAGTAG TTCAAGCCAC ATCTCAGACA 
ACAAAAACAG AGCCGGGCAC GCAGCTGAAT GTCACCAGCC TACAGTCCGT GCACCTGACA 
CAGGAGGTAC AGCAGCAGCT CCAGCAGGTG CCTGTCCAGC ATGTGTACAC TAACCAGGTG 
CAGTATGTGG AAGGAGGAGA AGCAAACTAC ACCACCAGCA CCATTCGTTC CAGCAGTTTC 
CCCTACACAG AGACCCCTCT GTACACCCAG AGCAGTGCGG GACAGTACTA CGAGGCCACC 
CCTGCCTCTG GATCTGAAGC CGCATCCCCC GGCACGCCTC TCACCGTCTC TGTCACCTCA 
GGATCTCCAG GTGTGCCCAT GTTTGTGGCA GGGACGGGGC AGATTGTGGC CAACCCCACC 
ACAGCGGCAG GCAGTGGGGT CACTGCAGTA GTAGCAGCTG CGGGGACGGC AGCTAACGGC 
TCTGGAGAAT CTGCCGGAGA GGCTAACGGT AGTGGCGGCA GCTATGTGAT CCAAGGTGGC 
TACATGTTGG CCGGCACCGG GAGTGGTAGC AGTGGAAACG GTCACGGGTA CCCGCACCCC 
ACTCGCGCTT CTCCAGCCAC CCTGTCCTCT GGCCCTCAGG TGCAGTGGTT GTTGGATAAT 
TATGAGACAG CAGAAGGAGT GAGTTTGCCC AGGTCTACAC TTTACTGCCA CTACCTGCTG 
CACTGTCAGG AGCAGAAGCT GGAGCCAGTT AATGCTGCAT CCTTTGGGAA ACTGATCCGT 
TCTGTGTTCA TGGGCTTGAG GACCAGACGT CTGGGCACAC GAGGGAATTC CAAATATCAC 
TACTATGGCC TGCGGATCAA AGCGAGCTCC TCTCTGCTCC GGCTGATGGA GGACCAGCAG 
CACCTGGCCA TGAGACAGCA GCCCTTCTCC CAGAAACAGA GGTTGAAGCC AGTTCAGAAG 
GTGGAGGGAA TCACCAACGG GATGTCGTCA GGATCTGGAC AGCAGCAGCA GCAGCAGCAG 
AGCTCGGGTT TGTCGGACAT CAGCACACAG GTCCAGCAGT ACCAGCAGTT TCTCGATGCG 
TCCAAAGCTC TCCCAGAGTT TCCTGATGTT GACCTGCAGG GTAAAGCTTT ACCAGAGGGC 
ATTGAGCTGG AACATCTCAA GAGTTTTCAG CTGCTCTACA GAGAACACTG CGAAGCCATT 
CTGGACGTGA TGGTCAACCT TCAGTTCCCT TTGGTGGAAA CTCTTTGGAA AACGTTTTGG 
AGGTTCAGTG AAAATCAGGC AGGAGACTCG GCAACACTAG CTGTGCACGA TGAGTCGGAG 
AAGAGGCTAC CCAAGACATG CTTGGTGATT CTCTGTAAAT ATGAGCCGGT GCTTCGCTGG 
AGTCGTGACT GCGACAACAC ACTGTACCAA GGCCTGGTGG AGATGCTCAT CCCAGACGTT 
CTGAGACCTA TTCCCAGTGC CTTAACACAA GCCATCCGAA ACTTTGCCAA GAGTCTTGAG 
AGCTGGTTGA CTAATGCCAT GGTGAACATT CCTGAAGAGA TGGTTCGTGT CAAGGTGACA 
TCTGCAAGTG CGTTTGCTCA GACTCTGCGC AGATACACCT CTCTAAACCA CCTGGCTCAG 
GCAGCTCGAG CCGTCCTGCA AAACACTGCG CAGATCAACC AGATGCTCAG CGACCTCAAC 
AGAGTGGATT TTGCAAATGT GCAGGAACAG GCCTCATGGG TGTGCCGGTG TGAGGACCGG 
GTGGTGCAGC GGCTTGAGCA GGACTTTAAG CTGACCCTTC AGCAGCAGAA CTCTCTGGAG 
CAGTGGGCCG CCTGGCTGGA TGGGGTGGTC TCGCAGGTCC TAAAGCCCTA CCAGTCCAGC 
GCTGCTCTTC CAAAAGCAGC TAAACTCTTC TTGCTGAAGT GGAGCTTTTA CAGCTCTATG 
GTGATCCGAG ATCTGACTCT CCGCAGTGCT GCCAGCTTCG GCTCTTTTCA CCTAATCCGT 
CTGCTCTACG ATGAGTACAT GTACTATCTG ATAGAGCACA GGGTTGCCCA GGCCAAGGGT 
GAAACACCGA TTGCTGTCAT GGGAGAGTTT GCAAGTCTTG GCCGAAGTCT GAACCCACTT 
GATCCTGATA AAGAAGAGGA AGAGGAGGAG GAAGAAGAGA GTGATGATGA GTGTCAGGAG 
TTATCTCTGC AGTCAGACGT GGGTGCGATG GGGGACGAAT CTCTGGAACC GCCTGCTAAA 
CTGGCCCGAA CCGACCAGAG GGTGTTGTTC ACCACAGGAA GCATGGAAAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297596.1
CDS	330..3023
Translation	MATPAYVRELQQTSQPQGGSVTPTSGQASTVTAGPQQFLAELQTTGATPSAAPPTGRTTPPTSQAQKSQAIAQAPPTQAPPTQTQYVTAEIQSSPTQSSNAQNTPQYIVVTVTEGSLHSSDSVSDSSPPAAVVQTGVPTQVVQQVQSAQQRSVVQATSQTTKTEPGTQLNVTSLQSVHLTQEVQQQLQQVPVQHVYTNQVQYVEGGEANYTTSTIRSSSFPYTETPLYTQSSAGQYYEATPASGSEAASPGTPLTVSVTSGSPGVPMFVAGTGQIVANPTTAAGSGVTAVVAAAGTAANGSGESAGEANGSGGSYVIQGGYMLAGTGSGSSGNGHGYPHPTRASPATLSSGPQVQWLLDNYETAEGVSLPRSTLYCHYLLHCQEQKLEPVNAASFGKLIRSVFMGLRTRRLGTRGNSKYHYYGLRIKASSSLLRLMEDQQHLAMRQQPFSQKQRLKPVQKVEGITNGMSSGSGQQQQQQQSSGLSDISTQVQQYQQFLDASKALPEFPDVDLQGKALPEGIELEHLKSFQLLYREHCEAILDVMVNLQFPLVETLWKTFWRFSENQAGDSATLAVHDESEKRLPKTCLVILCKYEPVLRWSRDCDNTLYQGLVEMLIPDVLRPIPSALTQAIRNFAKSLESWLTNAMVNIPEEMVRVKVTSASAFAQTLRRYTSLNHLAQAARAVLQNTAQINQMLSDLNRVDFANVQEQASWVCRCEDRVVQRLEQDFKLTLQQQNSLEQWAAWLDGVVSQVLKPYQSSAALPKAAKLFLLKWSFYSSMVIRDLTLRSAASFGSFHLIRLLYDEYMYYLIEHRVAQAKGETPIAVMGEFASLGRSLNPLDPDKEEEEEEEEESDDECQELSLQSDVGAMGDESLEPPAKLARTDQRVLFTTGSMEN

Target ORF information:

RefSeq Version	XM_009299321.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio regulatory factor X, 1a (influences HLA class II expression) (rfx1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299321.2

ORF Insert Sequence:

ATGGCAACTC CAGCATACGT GAGGGAGCTG CAGCAGACCT CCCAGCCTCA GGGGGGCAGT 
GTCACTCCTA CATCAGGACA GGCCTCTACT GTGACGGCGG GTCCTCAGCA ATTCCTGGCT 
GAACTTCAGA CTACAGGAGC CACGCCCTCA GCAGCCCCGC CAACTGGTCG GACCACACCT 
CCCACCTCAC AAGCACAGAA GAGCCAGGCG ATTGCACAGG CTCCACCCAC ACAAGCCCCG 
CCCACTCAAA CTCAGTACGT CACGGCTGAG ATTCAGAGCT CACCTACACA GTCCAGCAAT 
GCTCAGAATA CACCGCAGTA CATTGTTGTC ACAGTTACAG AGGGTTCTCT ACACTCTAGT 
GACTCTGTGT CAGATTCCAG TCCTCCTGCT GCTGTGGTCC AGACTGGAGT TCCCACACAG 
GTGGTGCAGC AAGTACAGTC AGCCCAACAG AGGTCAGTAG TTCAAGCCAC ATCTCAGACA 
ACAAAAACAG AGCCGGGCAC GCAGCTGAAT GTCACCAGCC TACAGTCCGT GCACCTGACA 
CAGGAGGTAC AGCAGCAGCT CCAGCAGGTG CCTGTCCAGC ATGTGTACAC TAACCAGGTG 
CAGTATGTGG AAGGAGGAGA AGCAAACTAC ACCACCAGCA CCATTCGTTC CAGCAGTTTC 
CCCTACACAG AGACCCCTCT GTACACCCAG AGCAGTGCGG GACAGTACTA CGAGGCCACC 
CCTGCCTCTG GATCTGAAGC CGCATCCCCC GGCACGCCTC TCACCGTCTC TGTCACCTCA 
GGATCTCCAG GTGTGCCCAT GTTTGTGGCA GGGACGGGGC AGATTGTGGC CAACCCCACC 
ACAGCGGCAG GCAGTGGGGT CACTGCAGTA GTAGCAGCTG CGGGGACGGC AGCTAACGGC 
TCTGGAGAAT CTGCCGGAGA GGCTAACGGT AGTGGCGGCA GCTATGTGAT CCAAGGTGGC 
TACATGTTGG CCGGCACCGG GAGTGGTAGC AGTGGAAACG GTCACGGGTA CCCGCACCCC 
ACTCGCGCTT CTCCAGCCAC CCTGTCCTCT GGCCCTCAGG TGCAGTGGTT GTTGGATAAT 
TATGAGACAG CAGAAGGAGT GAGTTTGCCC AGGTCTACAC TTTACTGCCA CTACCTGCTG 
CACTGTCAGG AGCAGAAGCT GGAGCCAGTT AATGCTGCAT CCTTTGGGAA ACTGATCCGT 
TCTGTGTTCA TGGGCTTGAG GACCAGACGT CTGGGCACAC GAGGGAATTC CAAATATCAC 
TACTATGGCC TGCGGATCAA AGCGAGCTCC TCTCTGCTCC GGCTGATGGA GGACCAGCAG 
CACCTGGCCA TGAGACAGCA GCCCTTCTCC CAGAAACAGA GGTTGAAGCC AGTTCAGAAG 
GTGGAGGGAA TCACCAACGG GATGTCGTCA GGATCTGGAC AGCAGCAGCA GCAGCAGCAG 
AGCTCGGGTT TGTCGGACAT CAGCACACAG GTCCAGCAGT ACCAGCAGTT TCTCGATGCG 
TCCAAAGCTC TCCCAGAGTT TCCTGATGTT GACCTGCAGG GTAAAGCTTT ACCAGAGGGC 
ATTGAGCTGG AACATCTCAA GAGTTTTCAG CTGCTCTACA GAGAACACTG CGAAGCCATT 
CTGGACGTGA TGGTCAACCT TCAGTTCCCT TTGGTGGAAA CTCTTTGGAA AACGTTTTGG 
AGGTTCAGTG AAAATCAGGC AGGAGACTCG GCAACACTAG CTGTGCACGA TGAGTCGGAG 
AAGAGGCTAC CCAAGACATG CTTGGTGATT CTCTGTAAAT ATGAGCCGGT GCTTCGCTGG 
AGTCGTGACT GCGACAACAC ACTGTACCAA GGCCTGGTGG AGATGCTCAT CCCAGACGTT 
CTGAGACCTA TTCCCAGTGC CTTAACACAA GCCATCCGAA ACTTTGCCAA GAGTCTTGAG 
AGCTGGTTGA CTAATGCCAT GGTGAACATT CCTGAAGAGA TGGTTCGTGT CAAGGTGACA 
TCTGCAAGTG CGTTTGCTCA GACTCTGCGC AGATACACCT CTCTAAACCA CCTGGCTCAG 
GCAGCTCGAG CCGTCCTGCA AAACACTGCG CAGATCAACC AGATGCTCAG CGACCTCAAC 
AGAGTGGATT TTGCAAATGT GCAGGAACAG GCCTCATGGG TGTGCCGGTG TGAGGACCGG 
GTGGTGCAGC GGCTTGAGCA GGACTTTAAG CTGACCCTTC AGCAGCAGAA CTCTCTGGAG 
CAGTGGGCCG CCTGGCTGGA TGGGGTGGTC TCGCAGGTCC TAAAGCCCTA CCAGTCCAGC 
GCTGCTCTTC CAAAAGCAGC TAAACTCTTC TTGCTGAAGT GGAGCTTTTA CAGCTCTATG 
GTGATCCGAG ATCTGACTCT CCGCAGTGCT GCCAGCTTCG GCTCTTTTCA CCTAATCCGT 
CTGCTCTACG ATGAGTACAT GTACTATCTG ATAGAGCACA GGGTTGCCCA GGCCAAGGGT 
GAAACACCGA TTGCTGTCAT GGGAGAGTTT GCAAGTCTTG GCCGAAGTCT GAACCCACTT 
GATCCTGATA AAGAAGAGGA AGAGGAGGAG GAAGAAGAGA GTGATGATGA GTGTCAGGAG 
TTATCTCTGC AGTCAGACGT GGGTGCGATG GGGGACGAAT CTCTGGAACC GCCTGCTAAA 
CTGGCCCGAA CCGACCAGAG GGTGTTGTTC ACCACAGGAA GCATGGAAAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rfx1a ( XM_021468997.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324672.1 Danio rerio regulatory factor X, 1a (influences HLA class II expression) (rfx1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa141533
Clone ID Related Accession (Same CDS sequence)	XM_021468997.1
Accession Version	XM_021468997.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	MHC class II regulatory factor RFX1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACTC CAGCATACGT GAGGGAGCTG CAGCAGACCT CCCAGCCTCA GGGGGGCAGT 
GTCACTCCTA CATCAGGACA GGCCTCTACT GTGACGGCGG GTCCTCAGCA ATTCCTGGCT 
GAACTTCAGA CTACAGGAGC CACGCCCTCA GCAGCCCCGC CAACTGGTCG GACCACACCT 
CCCACCTCAC AAGCACAGAA GAGCCAGGCG ATTGCACAGG CTCCACCCAC ACAAGCCCCG 
CCCACTCAAA CTCAGTACGT CACGGCTGAG ATTCAGAGCT CACCTACACA GTCCAGCAAT 
GCTCAGAATA CACCGCAGTA CATTGTTGTC ACAGTTACAG AGGGTTCTCT ACACTCTAGT 
GACTCTGTGT CAGATTCCAG TCCTCCTGCT GCTGTGGTCC AGACTGGAGT TCCCACACAG 
GTGGTGCAGC AAGTACAGTC AGCCCAACAG AGGTCAGTAG TTCAAGCCAC ATCTCAGACA 
ACAAAAACAG AGCCGGGCAC GCAGCTGAAT GTCACCAGCC TACAGTCCGT GCACCTGACA 
CAGGAGGTAC AGCAGCAGCT CCAGCAGGTG CCTGTCCAGC ATGTGTACAC TAACCAGGTG 
CAGTATGTGG AAGGAGGAGA AGCAAACTAC ACCACCAGCA CCATTCGTTC CAGCAGTTTC 
CCCTACACAG AGACCCCTCT GTACACCCAG AGCAGTGCGG GACAGTACTA CGAGGCCACC 
CCTGCCTCTG GATCTGAAGC CGCATCCCCC GGCACGCCTC TCACCGTCTC TGTCACCTCA 
GGATCTCCAG GTGTGCCCAT GTTTGTGGCA GGGACGGGGC AGATTGTGGC CAACCCCACC 
ACAGCGGCAG GCAGTGGGGT CACTGCAGTA GTAGCAGCTG CGGGGACGGC AGCTAACGGC 
TCTGGAGAAT CTGCCGGAGA GGCTAACGGT AGTGGCGGCA GCTATGTGAT CCAAGGTGGC 
TACATGTTGG CCGGCACCGG GAGTGGTAGC AGTGGAAACG GTCACGGGTA CCCGCACCCC 
ACTCGCGCTT CTCCAGCCAC CGTGAGTATA AATGAAGGCG AGGACAATAG CGTGCCGTCG 
GCAGACAAAA AGCTGTCCTC TGGCCCTCAG GTGCAGTGGT TGTTGGATAA TTATGAGACA 
GCAGAAGGAG TGAGTTTGCC CAGGTCTACA CTTTACTGCC ACTACCTGCT GCACTGTCAG 
GAGCAGAAGC TGGAGCCAGT TAATGCTGCA TCCTTTGGGA AACTGATCCG TTCTGTGTTC 
ATGGGCTTGA GGACCAGACG TCTGGGCACA CGAGGGAATT CCAAATATCA CTACTATGGC 
CTGCGGATCA AAGCGAGCTC CTCTCTGCTC CGGCTGATGG AGGACCAGCA GCACCTGGCC 
ATGAGACAGC AGCCCTTCTC CCAGAAACAG AGGTTGAAGC CAGTTCAGAA GGTGGAGGGA 
ATCACCAACG GGATGTCGTC AGGATCTGGA CAGCAGCAGC AGCAGCAGCA GAGCTCGGGT 
TTGTCGGACA TCAGCACACA GGTCCAGCAG TACCAGCAGT TTCTCGATGC GTCCAAAGCT 
CTCCCAGAGT TTCCTGATGT TGACCTGCAG GGTAAAGCTT TACCAGAGGG CATTGAGCTG 
GAACATCTCA AGAGTTTTCA GCTGCTCTAC AGAGAACACT GCGAAGCCAT TCTGGACGTG 
ATGGTCAACC TTCAGTTCCC TTTGGTGGAA ACTCTTTGGA AAACGTTTTG GAGGTTCAGT 
GAAAATCAGG CAGGAGACTC GGCAACACTA GCTGTGCACG ATGAGTCGGA GAAGAGGCTA 
CCCAAGACAT GCTTGGTGAT TCTCTGTAAA TATGAGCCGG TGCTTCGCTG GAGTCGTGAC 
TGCGACAACA CACTGTACCA AGGCCTGGTG GAGATGCTCA TCCCAGACGT TCTGAGACCT 
ATTCCCAGTG CCTTAACACA AGCCATCCGA AACTTTGCCA AGAGTCTTGA GAGCTGGTTG 
ACTAATGCCA TGGTGAACAT TCCTGAAGAG ATGGTTCGTG TCAAGGTGAC ATCTGCAAGT 
GCGTTTGCTC AGACTCTGCG CAGATACACC TCTCTAAACC ACCTGGCTCA GGCAGCTCGA 
GCCGTCCTGC AAAACACTGC GCAGATCAAC CAGATGCTCA GCGACCTCAA CAGAGTGGAT 
TTTGCAAATG TGCAGGAACA GGCCTCATGG GTGTGCCGGT GTGAGGACCG GGTGGTGCAG 
CGGCTTGAGC AGGACTTTAA GCTGACCCTT CAGCAGCAGA ACTCTCTGGA GCAGTGGGCC 
GCCTGGCTGG ATGGGGTGGT CTCGCAGGTC CTAAAGCCCT ACCAGTCCAG CGCTGCTCTT 
CCAAAAGCAG CTAAACTCTT CTTGCTGAAG TGGAGCTTTT ACAGCTCTAT GGTGATCCGA 
GATCTGACTC TCCGCAGTGC TGCCAGCTTC GGCTCTTTTC ACCTAATCCG TCTGCTCTAC 
GATGAGTACA TGTACTATCT GATAGAGCAC AGGGTTGCCC AGGCCAAGGG TGAAACACCG 
ATTGCTGTCA TGGGAGAGTT TGCAAGTCTT GGCCGAAGTC TGAACCCACT TGATCCTGAT 
AAAGAAGAGG AAGAGGAGGA GGAAGAAGAG AGTGATGATG AGTGTCAGGA GTTATCTCTG 
CAGTCAGACG TGGGTGCGAT GGGGGACGAA TCTCTGGAAC CGCCTGCTAA ACTGGCCCGA 
ACCGACCAGA GGGTGTTGTT CACCACAGGA AGCATGGAAA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324672.1
CDS	300..3044
Translation	MATPAYVRELQQTSQPQGGSVTPTSGQASTVTAGPQQFLAELQTTGATPSAAPPTGRTTPPTSQAQKSQAIAQAPPTQAPPTQTQYVTAEIQSSPTQSSNAQNTPQYIVVTVTEGSLHSSDSVSDSSPPAAVVQTGVPTQVVQQVQSAQQRSVVQATSQTTKTEPGTQLNVTSLQSVHLTQEVQQQLQQVPVQHVYTNQVQYVEGGEANYTTSTIRSSSFPYTETPLYTQSSAGQYYEATPASGSEAASPGTPLTVSVTSGSPGVPMFVAGTGQIVANPTTAAGSGVTAVVAAAGTAANGSGESAGEANGSGGSYVIQGGYMLAGTGSGSSGNGHGYPHPTRASPATVSINEGEDNSVPSADKKLSSGPQVQWLLDNYETAEGVSLPRSTLYCHYLLHCQEQKLEPVNAASFGKLIRSVFMGLRTRRLGTRGNSKYHYYGLRIKASSSLLRLMEDQQHLAMRQQPFSQKQRLKPVQKVEGITNGMSSGSGQQQQQQQSSGLSDISTQVQQYQQFLDASKALPEFPDVDLQGKALPEGIELEHLKSFQLLYREHCEAILDVMVNLQFPLVETLWKTFWRFSENQAGDSATLAVHDESEKRLPKTCLVILCKYEPVLRWSRDCDNTLYQGLVEMLIPDVLRPIPSALTQAIRNFAKSLESWLTNAMVNIPEEMVRVKVTSASAFAQTLRRYTSLNHLAQAARAVLQNTAQINQMLSDLNRVDFANVQEQASWVCRCEDRVVQRLEQDFKLTLQQQNSLEQWAAWLDGVVSQVLKPYQSSAALPKAAKLFLLKWSFYSSMVIRDLTLRSAASFGSFHLIRLLYDEYMYYLIEHRVAQAKGETPIAVMGEFASLGRSLNPLDPDKEEEEEEEEESDDECQELSLQSDVGAMGDESLEPPAKLARTDQRVLFTTGSMEN

Target ORF information:

RefSeq Version	XM_021468997.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio regulatory factor X, 1a (influences HLA class II expression) (rfx1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468997.1

ORF Insert Sequence:

ATGGCAACTC CAGCATACGT GAGGGAGCTG CAGCAGACCT CCCAGCCTCA GGGGGGCAGT 
GTCACTCCTA CATCAGGACA GGCCTCTACT GTGACGGCGG GTCCTCAGCA ATTCCTGGCT 
GAACTTCAGA CTACAGGAGC CACGCCCTCA GCAGCCCCGC CAACTGGTCG GACCACACCT 
CCCACCTCAC AAGCACAGAA GAGCCAGGCG ATTGCACAGG CTCCACCCAC ACAAGCCCCG 
CCCACTCAAA CTCAGTACGT CACGGCTGAG ATTCAGAGCT CACCTACACA GTCCAGCAAT 
GCTCAGAATA CACCGCAGTA CATTGTTGTC ACAGTTACAG AGGGTTCTCT ACACTCTAGT 
GACTCTGTGT CAGATTCCAG TCCTCCTGCT GCTGTGGTCC AGACTGGAGT TCCCACACAG 
GTGGTGCAGC AAGTACAGTC AGCCCAACAG AGGTCAGTAG TTCAAGCCAC ATCTCAGACA 
ACAAAAACAG AGCCGGGCAC GCAGCTGAAT GTCACCAGCC TACAGTCCGT GCACCTGACA 
CAGGAGGTAC AGCAGCAGCT CCAGCAGGTG CCTGTCCAGC ATGTGTACAC TAACCAGGTG 
CAGTATGTGG AAGGAGGAGA AGCAAACTAC ACCACCAGCA CCATTCGTTC CAGCAGTTTC 
CCCTACACAG AGACCCCTCT GTACACCCAG AGCAGTGCGG GACAGTACTA CGAGGCCACC 
CCTGCCTCTG GATCTGAAGC CGCATCCCCC GGCACGCCTC TCACCGTCTC TGTCACCTCA 
GGATCTCCAG GTGTGCCCAT GTTTGTGGCA GGGACGGGGC AGATTGTGGC CAACCCCACC 
ACAGCGGCAG GCAGTGGGGT CACTGCAGTA GTAGCAGCTG CGGGGACGGC AGCTAACGGC 
TCTGGAGAAT CTGCCGGAGA GGCTAACGGT AGTGGCGGCA GCTATGTGAT CCAAGGTGGC 
TACATGTTGG CCGGCACCGG GAGTGGTAGC AGTGGAAACG GTCACGGGTA CCCGCACCCC 
ACTCGCGCTT CTCCAGCCAC CGTGAGTATA AATGAAGGCG AGGACAATAG CGTGCCGTCG 
GCAGACAAAA AGCTGTCCTC TGGCCCTCAG GTGCAGTGGT TGTTGGATAA TTATGAGACA 
GCAGAAGGAG TGAGTTTGCC CAGGTCTACA CTTTACTGCC ACTACCTGCT GCACTGTCAG 
GAGCAGAAGC TGGAGCCAGT TAATGCTGCA TCCTTTGGGA AACTGATCCG TTCTGTGTTC 
ATGGGCTTGA GGACCAGACG TCTGGGCACA CGAGGGAATT CCAAATATCA CTACTATGGC 
CTGCGGATCA AAGCGAGCTC CTCTCTGCTC CGGCTGATGG AGGACCAGCA GCACCTGGCC 
ATGAGACAGC AGCCCTTCTC CCAGAAACAG AGGTTGAAGC CAGTTCAGAA GGTGGAGGGA 
ATCACCAACG GGATGTCGTC AGGATCTGGA CAGCAGCAGC AGCAGCAGCA GAGCTCGGGT 
TTGTCGGACA TCAGCACACA GGTCCAGCAG TACCAGCAGT TTCTCGATGC GTCCAAAGCT 
CTCCCAGAGT TTCCTGATGT TGACCTGCAG GGTAAAGCTT TACCAGAGGG CATTGAGCTG 
GAACATCTCA AGAGTTTTCA GCTGCTCTAC AGAGAACACT GCGAAGCCAT TCTGGACGTG 
ATGGTCAACC TTCAGTTCCC TTTGGTGGAA ACTCTTTGGA AAACGTTTTG GAGGTTCAGT 
GAAAATCAGG CAGGAGACTC GGCAACACTA GCTGTGCACG ATGAGTCGGA GAAGAGGCTA 
CCCAAGACAT GCTTGGTGAT TCTCTGTAAA TATGAGCCGG TGCTTCGCTG GAGTCGTGAC 
TGCGACAACA CACTGTACCA AGGCCTGGTG GAGATGCTCA TCCCAGACGT TCTGAGACCT 
ATTCCCAGTG CCTTAACACA AGCCATCCGA AACTTTGCCA AGAGTCTTGA GAGCTGGTTG 
ACTAATGCCA TGGTGAACAT TCCTGAAGAG ATGGTTCGTG TCAAGGTGAC ATCTGCAAGT 
GCGTTTGCTC AGACTCTGCG CAGATACACC TCTCTAAACC ACCTGGCTCA GGCAGCTCGA 
GCCGTCCTGC AAAACACTGC GCAGATCAAC CAGATGCTCA GCGACCTCAA CAGAGTGGAT 
TTTGCAAATG TGCAGGAACA GGCCTCATGG GTGTGCCGGT GTGAGGACCG GGTGGTGCAG 
CGGCTTGAGC AGGACTTTAA GCTGACCCTT CAGCAGCAGA ACTCTCTGGA GCAGTGGGCC 
GCCTGGCTGG ATGGGGTGGT CTCGCAGGTC CTAAAGCCCT ACCAGTCCAG CGCTGCTCTT 
CCAAAAGCAG CTAAACTCTT CTTGCTGAAG TGGAGCTTTT ACAGCTCTAT GGTGATCCGA 
GATCTGACTC TCCGCAGTGC TGCCAGCTTC GGCTCTTTTC ACCTAATCCG TCTGCTCTAC 
GATGAGTACA TGTACTATCT GATAGAGCAC AGGGTTGCCC AGGCCAAGGG TGAAACACCG 
ATTGCTGTCA TGGGAGAGTT TGCAAGTCTT GGCCGAAGTC TGAACCCACT TGATCCTGAT 
AAAGAAGAGG AAGAGGAGGA GGAAGAAGAG AGTGATGATG AGTGTCAGGA GTTATCTCTG 
CAGTCAGACG TGGGTGCGAT GGGGGACGAA TCTCTGGAAC CGCCTGCTAA ACTGGCCCGA 
ACCGACCAGA GGGTGTTGTT CACCACAGGA AGCATGGAAA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rfx1a ( XM_021469000.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324675.1 Danio rerio regulatory factor X, 1a (influences HLA class II expression) (rfx1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa141534
Clone ID Related Accession (Same CDS sequence)	XM_021469000.1
Accession Version	XM_021469000.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2727bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	MHC class II regulatory factor RFX1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACTC CAGCATACGT GAGGGAGCTG CAGCAGACCT CCCAGCCTCA GGGGGGCAGT 
GTCACTCCTA CATCAGGACA GGCCTCTACT GTGACGGCGG GTCCTCAGCA ATTCCTGGCT 
GAACTTCAGA CTACAGGAGC CACGCCCTCA GCAGCCCCGC CAACTGGTCG GACCACACCT 
CCCACCTCAC AAGCACAGAA GAGCCAGGCG ATTGCACAGG CTCCACCCAC ACAAGCCCCG 
CCCACTCAAA CTCAGTACGT CACGGCTGAG ATTCAGAGCT CACCTACACA GTCCAGCAAT 
GCTCAGAATA CACCGCAGTA CATTGTTGTC ACAGTTACAG AGGGTTCTCT ACACTCTAGT 
GACTCTGTGT CAGATTCCAG TCCTCCTGCT GCTGTGGTCC AGACTGGAGT TCCCACACAG 
GTGGTGCAGC AAGTACAGTC AGCCCAACAG AGGTCAGTAG TTCAAGCCAC ATCTCAGACA 
ACAAAAACAG AGCCGGGCAC GCAGCTGAAT GTCACCAGCC TACAGTCCGT GCACCTGACA 
CAGGAGGTAC AGCAGCAGCT CCAGCAGGTG CCTGTCCAGC ATGTGTACAC TAACCAGGTG 
CAGTATGTGG AAGGAGGAGA AGCAAACTAC ACCACCAGCA CCATTCGTTC CAGCAGTTTC 
CCCTACACAG AGACCCCTCT GTACACCCAG AGCAGTGCGG GACAGTACTA CGAGGCCACC 
CCTGCCTCTG GATCTGAAGC CGCATCCCCC GGCACGCCTC TCACCGTCTC TGTCACCTCA 
GGATCTCCAG GTGTGCCCAT GTTTGTGGCA GGGACGGGGC AGATTGTGGC CAACCCCACC 
ACAGCGGCAG GCAGTGGGGT CACTGCAGTA GTAGCAGCTG CGGGGACGGC AGCTAACGGC 
TCTGGAGAAT CTGCCGGAGA GGCTAACGGT AGTGGCGGCA GCTATGTGAT CCAAGGTGGC 
TACATGTTGG CCGGCACCGG GAGTGGTAGC AGTGGAAACG GTCACGGGTA CCCGCACCCC 
ACTCGCGCTT CTCCAGCCAC CGTGAGTATA AATGAAGGCG AGGACAATAG CGTGCCGTCG 
GCAGACAAAA AGGTGCAGTG GTTGTTGGAT AATTATGAGA CAGCAGAAGG AGTGAGTTTG 
CCCAGGTCTA CACTTTACTG CCACTACCTG CTGCACTGTC AGGAGCAGAA GCTGGAGCCA 
GTTAATGCTG CATCCTTTGG GAAACTGATC CGTTCTGTGT TCATGGGCTT GAGGACCAGA 
CGTCTGGGCA CACGAGGGAA TTCCAAATAT CACTACTATG GCCTGCGGAT CAAAGCGAGC 
TCCTCTCTGC TCCGGCTGAT GGAGGACCAG CAGCACCTGG CCATGAGACA GCAGCCCTTC 
TCCCAGAAAC AGAGGTTGAA GCCAGTTCAG AAGGTGGAGG GAATCACCAA CGGGATGTCG 
TCAGGATCTG GACAGCAGCA GCAGCAGCAG CAGAGCTCGG GTTTGTCGGA CATCAGCACA 
CAGGTCCAGC AGTACCAGCA GTTTCTCGAT GCGTCCAAAG CTCTCCCAGA GTTTCCTGAT 
GTTGACCTGC AGGGTAAAGC TTTACCAGAG GGCATTGAGC TGGAACATCT CAAGAGTTTT 
CAGCTGCTCT ACAGAGAACA CTGCGAAGCC ATTCTGGACG TGATGGTCAA CCTTCAGTTC 
CCTTTGGTGG AAACTCTTTG GAAAACGTTT TGGAGGTTCA GTGAAAATCA GGCAGGAGAC 
TCGGCAACAC TAGCTGTGCA CGATGAGTCG GAGAAGAGGC TACCCAAGAC ATGCTTGGTG 
ATTCTCTGTA AATATGAGCC GGTGCTTCGC TGGAGTCGTG ACTGCGACAA CACACTGTAC 
CAAGGCCTGG TGGAGATGCT CATCCCAGAC GTTCTGAGAC CTATTCCCAG TGCCTTAACA 
CAAGCCATCC GAAACTTTGC CAAGAGTCTT GAGAGCTGGT TGACTAATGC CATGGTGAAC 
ATTCCTGAAG AGATGGTTCG TGTCAAGGTG ACATCTGCAA GTGCGTTTGC TCAGACTCTG 
CGCAGATACA CCTCTCTAAA CCACCTGGCT CAGGCAGCTC GAGCCGTCCT GCAAAACACT 
GCGCAGATCA ACCAGATGCT CAGCGACCTC AACAGAGTGG ATTTTGCAAA TGTGCAGGAA 
CAGGCCTCAT GGGTGTGCCG GTGTGAGGAC CGGGTGGTGC AGCGGCTTGA GCAGGACTTT 
AAGCTGACCC TTCAGCAGCA GAACTCTCTG GAGCAGTGGG CCGCCTGGCT GGATGGGGTG 
GTCTCGCAGG TCCTAAAGCC CTACCAGTCC AGCGCTGCTC TTCCAAAAGC AGCTAAACTC 
TTCTTGCTGA AGTGGAGCTT TTACAGCTCT ATGGTGATCC GAGATCTGAC TCTCCGCAGT 
GCTGCCAGCT TCGGCTCTTT TCACCTAATC CGTCTGCTCT ACGATGAGTA CATGTACTAT 
CTGATAGAGC ACAGGGTTGC CCAGGCCAAG GGTGAAACAC CGATTGCTGT CATGGGAGAG 
TTTGCAAGTC TTGGCCGAAG TCTGAACCCA CTTGATCCTG ATAAAGAAGA GGAAGAGGAG 
GAGGAAGAAG AGAGTGATGA TGAGTGTCAG GAGTTATCTC TGCAGTCAGA CGTGGGTGCG 
ATGGGGGACG AATCTCTGGA ACCGCCTGCT AAACTGGCCC GAACCGACCA GAGGGTGTTG 
TTCACCACAG GAAGCATGGA AAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324675.1
CDS	300..3026
Translation	MATPAYVRELQQTSQPQGGSVTPTSGQASTVTAGPQQFLAELQTTGATPSAAPPTGRTTPPTSQAQKSQAIAQAPPTQAPPTQTQYVTAEIQSSPTQSSNAQNTPQYIVVTVTEGSLHSSDSVSDSSPPAAVVQTGVPTQVVQQVQSAQQRSVVQATSQTTKTEPGTQLNVTSLQSVHLTQEVQQQLQQVPVQHVYTNQVQYVEGGEANYTTSTIRSSSFPYTETPLYTQSSAGQYYEATPASGSEAASPGTPLTVSVTSGSPGVPMFVAGTGQIVANPTTAAGSGVTAVVAAAGTAANGSGESAGEANGSGGSYVIQGGYMLAGTGSGSSGNGHGYPHPTRASPATVSINEGEDNSVPSADKKVQWLLDNYETAEGVSLPRSTLYCHYLLHCQEQKLEPVNAASFGKLIRSVFMGLRTRRLGTRGNSKYHYYGLRIKASSSLLRLMEDQQHLAMRQQPFSQKQRLKPVQKVEGITNGMSSGSGQQQQQQQSSGLSDISTQVQQYQQFLDASKALPEFPDVDLQGKALPEGIELEHLKSFQLLYREHCEAILDVMVNLQFPLVETLWKTFWRFSENQAGDSATLAVHDESEKRLPKTCLVILCKYEPVLRWSRDCDNTLYQGLVEMLIPDVLRPIPSALTQAIRNFAKSLESWLTNAMVNIPEEMVRVKVTSASAFAQTLRRYTSLNHLAQAARAVLQNTAQINQMLSDLNRVDFANVQEQASWVCRCEDRVVQRLEQDFKLTLQQQNSLEQWAAWLDGVVSQVLKPYQSSAALPKAAKLFLLKWSFYSSMVIRDLTLRSAASFGSFHLIRLLYDEYMYYLIEHRVAQAKGETPIAVMGEFASLGRSLNPLDPDKEEEEEEEEESDDECQELSLQSDVGAMGDESLEPPAKLARTDQRVLFTTGSMEN

Target ORF information:

RefSeq Version	XM_021469000.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio regulatory factor X, 1a (influences HLA class II expression) (rfx1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469000.1

ORF Insert Sequence:

ATGGCAACTC CAGCATACGT GAGGGAGCTG CAGCAGACCT CCCAGCCTCA GGGGGGCAGT 
GTCACTCCTA CATCAGGACA GGCCTCTACT GTGACGGCGG GTCCTCAGCA ATTCCTGGCT 
GAACTTCAGA CTACAGGAGC CACGCCCTCA GCAGCCCCGC CAACTGGTCG GACCACACCT 
CCCACCTCAC AAGCACAGAA GAGCCAGGCG ATTGCACAGG CTCCACCCAC ACAAGCCCCG 
CCCACTCAAA CTCAGTACGT CACGGCTGAG ATTCAGAGCT CACCTACACA GTCCAGCAAT 
GCTCAGAATA CACCGCAGTA CATTGTTGTC ACAGTTACAG AGGGTTCTCT ACACTCTAGT 
GACTCTGTGT CAGATTCCAG TCCTCCTGCT GCTGTGGTCC AGACTGGAGT TCCCACACAG 
GTGGTGCAGC AAGTACAGTC AGCCCAACAG AGGTCAGTAG TTCAAGCCAC ATCTCAGACA 
ACAAAAACAG AGCCGGGCAC GCAGCTGAAT GTCACCAGCC TACAGTCCGT GCACCTGACA 
CAGGAGGTAC AGCAGCAGCT CCAGCAGGTG CCTGTCCAGC ATGTGTACAC TAACCAGGTG 
CAGTATGTGG AAGGAGGAGA AGCAAACTAC ACCACCAGCA CCATTCGTTC CAGCAGTTTC 
CCCTACACAG AGACCCCTCT GTACACCCAG AGCAGTGCGG GACAGTACTA CGAGGCCACC 
CCTGCCTCTG GATCTGAAGC CGCATCCCCC GGCACGCCTC TCACCGTCTC TGTCACCTCA 
GGATCTCCAG GTGTGCCCAT GTTTGTGGCA GGGACGGGGC AGATTGTGGC CAACCCCACC 
ACAGCGGCAG GCAGTGGGGT CACTGCAGTA GTAGCAGCTG CGGGGACGGC AGCTAACGGC 
TCTGGAGAAT CTGCCGGAGA GGCTAACGGT AGTGGCGGCA GCTATGTGAT CCAAGGTGGC 
TACATGTTGG CCGGCACCGG GAGTGGTAGC AGTGGAAACG GTCACGGGTA CCCGCACCCC 
ACTCGCGCTT CTCCAGCCAC CGTGAGTATA AATGAAGGCG AGGACAATAG CGTGCCGTCG 
GCAGACAAAA AGGTGCAGTG GTTGTTGGAT AATTATGAGA CAGCAGAAGG AGTGAGTTTG 
CCCAGGTCTA CACTTTACTG CCACTACCTG CTGCACTGTC AGGAGCAGAA GCTGGAGCCA 
GTTAATGCTG CATCCTTTGG GAAACTGATC CGTTCTGTGT TCATGGGCTT GAGGACCAGA 
CGTCTGGGCA CACGAGGGAA TTCCAAATAT CACTACTATG GCCTGCGGAT CAAAGCGAGC 
TCCTCTCTGC TCCGGCTGAT GGAGGACCAG CAGCACCTGG CCATGAGACA GCAGCCCTTC 
TCCCAGAAAC AGAGGTTGAA GCCAGTTCAG AAGGTGGAGG GAATCACCAA CGGGATGTCG 
TCAGGATCTG GACAGCAGCA GCAGCAGCAG CAGAGCTCGG GTTTGTCGGA CATCAGCACA 
CAGGTCCAGC AGTACCAGCA GTTTCTCGAT GCGTCCAAAG CTCTCCCAGA GTTTCCTGAT 
GTTGACCTGC AGGGTAAAGC TTTACCAGAG GGCATTGAGC TGGAACATCT CAAGAGTTTT 
CAGCTGCTCT ACAGAGAACA CTGCGAAGCC ATTCTGGACG TGATGGTCAA CCTTCAGTTC 
CCTTTGGTGG AAACTCTTTG GAAAACGTTT TGGAGGTTCA GTGAAAATCA GGCAGGAGAC 
TCGGCAACAC TAGCTGTGCA CGATGAGTCG GAGAAGAGGC TACCCAAGAC ATGCTTGGTG 
ATTCTCTGTA AATATGAGCC GGTGCTTCGC TGGAGTCGTG ACTGCGACAA CACACTGTAC 
CAAGGCCTGG TGGAGATGCT CATCCCAGAC GTTCTGAGAC CTATTCCCAG TGCCTTAACA 
CAAGCCATCC GAAACTTTGC CAAGAGTCTT GAGAGCTGGT TGACTAATGC CATGGTGAAC 
ATTCCTGAAG AGATGGTTCG TGTCAAGGTG ACATCTGCAA GTGCGTTTGC TCAGACTCTG 
CGCAGATACA CCTCTCTAAA CCACCTGGCT CAGGCAGCTC GAGCCGTCCT GCAAAACACT 
GCGCAGATCA ACCAGATGCT CAGCGACCTC AACAGAGTGG ATTTTGCAAA TGTGCAGGAA 
CAGGCCTCAT GGGTGTGCCG GTGTGAGGAC CGGGTGGTGC AGCGGCTTGA GCAGGACTTT 
AAGCTGACCC TTCAGCAGCA GAACTCTCTG GAGCAGTGGG CCGCCTGGCT GGATGGGGTG 
GTCTCGCAGG TCCTAAAGCC CTACCAGTCC AGCGCTGCTC TTCCAAAAGC AGCTAAACTC 
TTCTTGCTGA AGTGGAGCTT TTACAGCTCT ATGGTGATCC GAGATCTGAC TCTCCGCAGT 
GCTGCCAGCT TCGGCTCTTT TCACCTAATC CGTCTGCTCT ACGATGAGTA CATGTACTAT 
CTGATAGAGC ACAGGGTTGC CCAGGCCAAG GGTGAAACAC CGATTGCTGT CATGGGAGAG 
TTTGCAAGTC TTGGCCGAAG TCTGAACCCA CTTGATCCTG ATAAAGAAGA GGAAGAGGAG 
GAGGAAGAAG AGAGTGATGA TGAGTGTCAG GAGTTATCTC TGCAGTCAGA CGTGGGTGCG 
ATGGGGGACG AATCTCTGGA ACCGCCTGCT AAACTGGCCC GAACCGACCA GAGGGTGTTG 
TTCACCACAG GAAGCATGGA AAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Function-driven discovery of disease genes in zebrafish using an integrated genomics big data resource.
Nucleic acids research44(20)9611-9623(2016 Nov)
Shim H,Kim JH,Kim CY,Hwang S,Kim H,Yang S,Lee JE,Lee I

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Mutation of kri1l causes definitive hematopoiesis failure via PERK-dependent excessive autophagy induction.
Cell research25(8)946-62(2015 Aug)
Jia XE,Ma K,Xu T,Gao L,Wu S,Fu C,Zhang W,Wang Z,Liu K,Dong M,Jing C,Ren C,Dong Z,Chen Y,Jin Y,Huang Q,Chang X,Deng M,Li L,Luo L,Zhu J,Dang Y,Chang HC,Zon LI,Zhou Y,Chen S,Pan W

Rfx6 is an Ngn3-dependent winged helix transcription factor required for pancreatic islet cell development.
Development (Cambridge, England)137(2)203-12(2010 Jan)
Soyer J,Flasse L,Raffelsberger W,Beucher A,Orvain C,Peers B,Ravassard P,Vermot J,Voz ML,Mellitzer G,Gradwohl G

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rfx1a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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