grhl2b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	grhl2b
Entrez Gene ID	100034503
Full Name	grainyhead-like transcription factor 2b
Synonyms	grhl2,si:dkey-21k18.2
General protein information	Preferred Names grainyhead-like transcription factor 2b Names grainyhead-like protein 2 homolog grainyhead-like 2 grainyhead-like 2b protein
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005159580.3	XP_005159637.1	grainyhead-like protein 2 homolog isoform X1
XM_009294097.2	XP_009292372.1	grainyhead-like protein 2 homolog isoform X2
XM_005159580.4	XP_005159637.1	grainyhead-like protein 2 homolog isoform X1
NM_001083072.1	NP_001076541.1	grainyhead-like protein 2 homolog

Pathways from BioSystems

Homologies

Homo sapiens (human)	GRHL2	NP_079191.2
	GRHL2	XP_001098439.2
Canis lupus familiaris (dog)	GRHL2	XP_005627890.1
Gallus gallus (chicken)	GRHL2	XP_004940038.1
Xenopus tropicalis (tropical clawed frog)	grhl2	NP_001011338.1
Pan troglodytes (chimpanzee)	GRHL2	XP_519890.3
Mus musculus (house mouse)	Grhl2	NP_080772.2
Bos taurus (cattle)	GRHL2	XP_002692840.1
Rattus norvegicus (Norway rat)	Grhl2	NP_001127999.1
Danio rerio (zebrafish)	grhl2b	NP_001076541.1

Gene Ontology
Bibliography

Related articles in PubMed

Mis-expression of grainyhead-like transcription factors in zebrafish leads to defects in enveloping layer (EVL) integrity, cellular morphogenesis and axial extension.
Miles LB, Darido C, Kaslin J, Heath JK, Jane SM, Dworkin S
Scientific reports7(1)17607(2017 Dec)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

High-throughput gene targeting and phenotyping in zebrafish using CRISPR/Cas9.
Varshney GK, Pei W, LaFave MC, Idol J, Xu L, Gallardo V, Carrington B, Bishop K, Jones M, Li M, Harper U, Huang SC, Prakash A, Chen W, Sood R, Ledin J, Burgess SM
Genome research25(7)1030-42(2015 Jul)

Grhl1 deficiency affects inner ear development in zebrafish.
Liu F, Yang F, Wen D, Xia W, Hao L, Hu J, Zong J, Shen X, Ma J, Jiang N, Sun S, Zhang J, Wang H, Wang X, Ma Z, Ma D
The International journal of developmental biology59(10-12)417-23(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The data showed that midbrain-hindbrain boundary maintenance and morphogenesis are dissociable events regulated by grhl2b through diverse transcriptional targets.
Title: Midbrain-hindbrain boundary patterning and morphogenesis are regulated by diverse grainy head-like 2-dependent pathways.

This work reveals an evolutionarily conserved function of Grhl2 in otic development and provides a fish model for further studying mechanisms of Grhl2-related hearing loss.
Title: Grhl2 deficiency impairs otic development and hearing ability in a zebrafish model of the progressive dominant hearing loss DFNA28.

The following grhl2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the grhl2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa35880	NM_001083072.1 Latest version!	Danio rerio grainyhead-like transcription factor 2b (grhl2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa23993	XM_005159580.4 Latest version!	Danio rerio grainyhead-like transcription factor 2b (grhl2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa48381	XM_009294097.2 Latest version!	Danio rerio grainyhead-like transcription factor 2b (grhl2b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
View Old Accession Versions >>
ODa23993	XM_005159580.3	Danio rerio grainyhead-like transcription factor 2b (grhl2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

grhl2b ( NM_001083072.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001076541.1 Danio rerio grainyhead-like transcription factor 2b (grhl2b), mRNA.

Price & Availability↑

CloneID	ODa35880
Clone ID Related Accession (Same CDS sequence)	NM_001083072.1
Accession Version	NM_001083072.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	grainyhead-like protein 2 homolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX294657.11. ##Evidence-Data-START## CDS exon combination :: GDQH01021635.1, GFIL01011948.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGATGAGCA TCAATGGAGA TGAAGACAGT GCTGCCGCCC TGGGGCTCCT TTATGAGTAC 
TACAAGGTGC CCAAAGATAA GAGAGTACCT ATCAGCAAAG TGGTTGAAAT GTCAGACGAA 
CCGGAAAAGA GAAGCACACC AGTGGGAAAC AGCAGCCAAA TGGAGCCGGA GACGGTGGAT 
AATCATGTCC AAGTCCTGAA ATCAGTGCCT GTTAATCTGT CACTCAACAC GGATCACCAG 
GACAATAAAC GGGAACAGTA CAGCGTGCCG GCTGGAGAGA GCGGGACGGC TGCTGTGGTA 
AAAGCTGAAG CGCACACTGC TGTCTTTATG ACTTCAGGGG GACACTACAG GACAGAAGGG 
GAGGAGCAGA TGCGGGTCAT CTGTGAACAG ACCCACTCAT TCGACACCTC CACCATAAAT 
CACAGTGCAT ATGGAAAAGA TGACCATCGC AGTAGTCCAG AAAGCACATA TGAAGATGAC 
AGCAATGAAA AGTACCGCTC GTCTTCGATT GGACCTGATG ACTTCATTTT TGACCAGTCA 
CATATGTTAG ACACATTTCA ATACACACTA GAGGCCAGCA AGTCATTGCG GCAAAAGCAG 
GGCGAGGGGC CTATGACTTA TCTGAACAAG GGCCAGTTCT ATGCTATTAC ACTAAGTGAG 
ACAAGTGCTA ATAAACGCCT TCGACATCCC ATCAGCAAAG TGAGGAGTGT CGTCATGGTT 
GTATTCAGTG AGGATAAGAA TCGAGATGAG CAACTTAAAT ACTGGAAGTA TTGGCACTCC 
CGCCAGCACA CGGCGAAGCA GAGGGTTCTG GACATTGCTG ACTACAAGGA GAGCTTCAAC 
ACAATTGGAA ATATTGAAGA AATTGCATAC AACGCTGTGT CTTTCACCTG GGACCTGAAC 
GAGGAGGCCA AGATCTTCAT CACGGTGAAC TGTCTAAGCA CAGATTTCTC CTCTCAGAAG 
GGGGTGAAAG GTCTTCCTCT GATGATACAG ATTGACACCT ACAGCTACAA CAACCGCAGT 
AACAAACCCC TGCACAGAGC CTACTGCCAG ATCAAGGTCT TCTGTGACAA GGGAGCGGAG 
AGGAAGATCA GAGATGAGGA GAGGAAACAA AACCGCAAGA AGACAAAAGG AAAGGATGGA 
GGCAGCGGGA CCGTTACTGT CCCGAAAAAA TGTGACAGCA CCCTCTTCAA AACGATGACT 
GATTTGGATG CCCAGCCAGT TCTCTTTATC CCGGATGTGC ACTTTGGGAA CCTACAGAGG 
GCAGGCCAGG TATTTGCATT CAATACAGAG GAAATTGAAT GTGAGGGGAG TGTGCTGGTG 
AAGAGGATGT TCCGGTCATC TGAGGAAGAC TTCTGCCCAC CGCCACATAA ACAGCTCAGA 
GAAGACACAC AGAAGAGAGT GCTGTTGTAC GTGAGGAAGG AGTCAGACGA GGTGTTTGAT 
GCTCTGATGT TGCGGTCGCC CACTGTCAGA GGACTCATGG ATGCAATTTC TGAGAAGTAT 
GGTGTTCCCA TTGACAGAAT GGCTAAGATT TACAAAAAGA GCAAGAAAGG GATTCTGGTG 
AACATGGACG ACAACATCAT CGAGCACTAC TCAAACGAGG ACACGTTTAT CCTGAATATC 
GAGAGCTACG CAGACGCATA CAAAATCACT TTAACGGAAA TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001076541.1
CDS	122..1786
Translation	MMSINGDEDSAAALGLLYEYYKVPKDKRVPISKVVEMSDEPEKRSTPVGNSSQMEPETVDNHVQVLKSVPVNLSLNTDHQDNKREQYSVPAGESGTAAVVKAEAHTAVFMTSGGHYRTEGEEQMRVICEQTHSFDTSTINHSAYGKDDHRSSPESTYEDDSNEKYRSSSIGPDDFIFDQSHMLDTFQYTLEASKSLRQKQGEGPMTYLNKGQFYAITLSETSANKRLRHPISKVRSVVMVVFSEDKNRDEQLKYWKYWHSRQHTAKQRVLDIADYKESFNTIGNIEEIAYNAVSFTWDLNEEAKIFITVNCLSTDFSSQKGVKGLPLMIQIDTYSYNNRSNKPLHRAYCQIKVFCDKGAERKIRDEERKQNRKKTKGKDGGSGTVTVPKKCDSTLFKTMTDLDAQPVLFIPDVHFGNLQRAGQVFAFNTEEIECEGSVLVKRMFRSSEEDFCPPPHKQLREDTQKRVLLYVRKESDEVFDALMLRSPTVRGLMDAISEKYGVPIDRMAKIYKKSKKGILVNMDDNIIEHYSNEDTFILNIESYADAYKITLTEI

Target ORF information:

RefSeq Version	NM_001083072.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio grainyhead-like transcription factor 2b (grhl2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083072.1

ORF Insert Sequence:

ATGATGAGCA TCAATGGAGA TGAAGACAGT GCTGCCGCCC TGGGGCTCCT TTATGAGTAC 
TACAAGGTGC CCAAAGATAA GAGAGTACCT ATCAGCAAAG TGGTTGAAAT GTCAGACGAA 
CCGGAAAAGA GAAGCACACC AGTGGGAAAC AGCAGCCAAA TGGAGCCGGA GACGGTGGAT 
AATCATGTCC AAGTCCTGAA ATCAGTGCCT GTTAATCTGT CACTCAACAC GGATCACCAG 
GACAATAAAC GGGAACAGTA CAGCGTGCCG GCTGGAGAGA GCGGGACGGC TGCTGTGGTA 
AAAGCTGAAG CGCACACTGC TGTCTTTATG ACTTCAGGGG GACACTACAG GACAGAAGGG 
GAGGAGCAGA TGCGGGTCAT CTGTGAACAG ACCCACTCAT TCGACACCTC CACCATAAAT 
CACAGTGCAT ATGGAAAAGA TGACCATCGC AGTAGTCCAG AAAGCACATA TGAAGATGAC 
AGCAATGAAA AGTACCGCTC GTCTTCGATT GGACCTGATG ACTTCATTTT TGACCAGTCA 
CATATGTTAG ACACATTTCA ATACACACTA GAGGCCAGCA AGTCATTGCG GCAAAAGCAG 
GGCGAGGGGC CTATGACTTA TCTGAACAAG GGCCAGTTCT ATGCTATTAC ACTAAGTGAG 
ACAAGTGCTA ATAAACGCCT TCGACATCCC ATCAGCAAAG TGAGGAGTGT CGTCATGGTT 
GTATTCAGTG AGGATAAGAA TCGAGATGAG CAACTTAAAT ACTGGAAGTA TTGGCACTCC 
CGCCAGCACA CGGCGAAGCA GAGGGTTCTG GACATTGCTG ACTACAAGGA GAGCTTCAAC 
ACAATTGGAA ATATTGAAGA AATTGCATAC AACGCTGTGT CTTTCACCTG GGACCTGAAC 
GAGGAGGCCA AGATCTTCAT CACGGTGAAC TGTCTAAGCA CAGATTTCTC CTCTCAGAAG 
GGGGTGAAAG GTCTTCCTCT GATGATACAG ATTGACACCT ACAGCTACAA CAACCGCAGT 
AACAAACCCC TGCACAGAGC CTACTGCCAG ATCAAGGTCT TCTGTGACAA GGGAGCGGAG 
AGGAAGATCA GAGATGAGGA GAGGAAACAA AACCGCAAGA AGACAAAAGG AAAGGATGGA 
GGCAGCGGGA CCGTTACTGT CCCGAAAAAA TGTGACAGCA CCCTCTTCAA AACGATGACT 
GATTTGGATG CCCAGCCAGT TCTCTTTATC CCGGATGTGC ACTTTGGGAA CCTACAGAGG 
GCAGGCCAGG TATTTGCATT CAATACAGAG GAAATTGAAT GTGAGGGGAG TGTGCTGGTG 
AAGAGGATGT TCCGGTCATC TGAGGAAGAC TTCTGCCCAC CGCCACATAA ACAGCTCAGA 
GAAGACACAC AGAAGAGAGT GCTGTTGTAC GTGAGGAAGG AGTCAGACGA GGTGTTTGAT 
GCTCTGATGT TGCGGTCGCC CACTGTCAGA GGACTCATGG ATGCAATTTC TGAGAAGTAT 
GGTGTTCCCA TTGACAGAAT GGCTAAGATT TACAAAAAGA GCAAGAAAGG GATTCTGGTG 
AACATGGACG ACAACATCAT CGAGCACTAC TCAAACGAGG ACACGTTTAT CCTGAATATC 
GAGAGCTACG CAGACGCATA CAAAATCACT TTAACGGAAA TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

grhl2b ( XM_005159580.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159637.1 Danio rerio grainyhead-like transcription factor 2b (grhl2b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa23993
Clone ID Related Accession (Same CDS sequence)	XM_005159580.3 , XM_005159580.4
Accession Version	XM_005159580.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1806bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	grainyhead-like protein 2 homolog isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159580.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACAGG AGACAGACAG TAAGAGGCTG GTTGTAGTGG TGCCCAATGA GCCGTCTTTC 
CACCAGCGGC GGCCCTACAC GAGCGAGGAT GAGGCCTGGA GGTCTTACCT GGAGAATCCA 
CTTACTGCGG CCACAAAGGC TATGATGAGC ATCAATGGAG ATGAAGACAG TGCTGCCGCC 
CTGGGGCTCC TTTATGAGTA CTACAAGGTG CCCAAAGATA AGAGAGTACC TATCAGCAAA 
GTGGTTGAAA TGTCAGACGA ACCGGAAAAG AGAAGCACAC CAGTGGGAAA CAGCAGCCAA 
ATGGAGCCGG AGACGGTGGA TAATCATGTC CAAGTCCTGA AATCAGTGCC TGTTAATCTG 
TCACTCAACA CGGATCACCA GGACAATAAA CGGGAACAGT ACAGCGTGCC GGCTGGAGAG 
AGCGGGACGG CTGCTGTGGT AAAAGCTGAA GCGCACACTG CTGTCTTTAT GACTTCAGGG 
GGACACTACA GGACAGAAGG GGAGGAGCAG ATGCGGGTCA TCTGTGAACA GACCCACTCA 
TTCGACACCT CCACCATAAA TCACAGTGCA TATGGAAAAG ATGACCATCG CAGTAGTCCA 
GAAAGCACAT ATGAAGATGA CAGCAATGAA AAGTACCGCT CGTCTTCGAT TGGACCTGAT 
GACTTCATTT TTGACCAGTC ACATATGTTA GACACATTTC AATACACACT AGAGGCCAGC 
AAGTCATTGC GGCAAAAGCA GGGCGAGGGG CCTATGACTT ATCTGAACAA GGGCCAGTTC 
TATGCTATTA CACTAAGTGA GACAAGTGCT AATAAACGCC TTCGACATCC CATCAGCAAA 
GTGAGGAGTG TCGTCATGGT TGTATTCAGT GAGGATAAGA ATCGAGATGA GCAACTTAAA 
TACTGGAAGT ATTGGCACTC CCGCCAGCAC ACGGCGAAGC AGAGGGTTCT GGACATTGCT 
GACTACAAGG AGAGCTTCAA CACAATTGGA AATATTGAAG AAATTGCATA CAACGCTGTG 
TCTTTCACCT GGGACCTGAA CGAGGAGGCC AAGATCTTCA TCACGGTGAA CTGTCTAAGC 
ACAGATTTCT CCTCTCAGAA GGGGGTGAAA GGTCTTCCTC TGATGATACA GATTGACACC 
TACAGCTACA ACAACCGCAG TAACAAACCC CTGCACAGAG CCTACTGCCA GATCAAGGTC 
TTCTGTGACA AGGGAGCGGA GAGGAAGATC AGAGATGAGG AGAGGAAACA AAACCGCAAG 
AAGACAAAAG GAAAGGATGG AGGCAGCGGG ACCGTTACTG TCCCGAAAAA ATGTGACAGC 
ACCCTCTTCA AAACGATGAC TGATTTGGAT GCCCAGCCAG TTCTCTTTAT CCCGGATGTG 
CACTTTGGGA ACCTACAGAG GGCAGGCCAG GTATTTGCAT TCAATACAGA GGAAATTGAA 
TGTGAGGGGA GTGTGCTGGT GAAGAGGATG TTCCGGTCAT CTGAGGAAGA CTTCTGCCCA 
CCGCCACATA AACAGCTCAG AGAAGACACA CAGAAGAGAG TGCTGTTGTA CGTGAGGAAG 
GAGTCAGACG AGGTGTTTGA TGCTCTGATG TTGCGGTCGC CCACTGTCAG AGGACTCATG 
GATGCAATTT CTGAGAAGTA TGGTGTTCCC ATTGACAGAA TGGCTAAGAT TTACAAAAAG 
AGCAAGAAAG GGATTCTGGT GAACATGGAC GACAACATCA TCGAGCACTA CTCAAACGAG 
GACACGTTTA TCCTGAATAT CGAGAGCTAC GCAGACGCAT ACAAAATCAC TTTAACGGAA 
ATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159637.1
CDS	283..2088
Translation	MSQETDSKRLVVVVPNEPSFHQRRPYTSEDEAWRSYLENPLTAATKAMMSINGDEDSAAALGLLYEYYKVPKDKRVPISKVVEMSDEPEKRSTPVGNSSQMEPETVDNHVQVLKSVPVNLSLNTDHQDNKREQYSVPAGESGTAAVVKAEAHTAVFMTSGGHYRTEGEEQMRVICEQTHSFDTSTINHSAYGKDDHRSSPESTYEDDSNEKYRSSSIGPDDFIFDQSHMLDTFQYTLEASKSLRQKQGEGPMTYLNKGQFYAITLSETSANKRLRHPISKVRSVVMVVFSEDKNRDEQLKYWKYWHSRQHTAKQRVLDIADYKESFNTIGNIEEIAYNAVSFTWDLNEEAKIFITVNCLSTDFSSQKGVKGLPLMIQIDTYSYNNRSNKPLHRAYCQIKVFCDKGAERKIRDEERKQNRKKTKGKDGGSGTVTVPKKCDSTLFKTMTDLDAQPVLFIPDVHFGNLQRAGQVFAFNTEEIECEGSVLVKRMFRSSEEDFCPPPHKQLREDTQKRVLLYVRKESDEVFDALMLRSPTVRGLMDAISEKYGVPIDRMAKIYKKSKKGILVNMDDNIIEHYSNEDTFILNIESYADAYKITLTEI

Target ORF information:

RefSeq Version	XM_005159580.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio grainyhead-like transcription factor 2b (grhl2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159580.3

ORF Insert Sequence:

ATGTCACAGG AGACAGACAG TAAGAGGCTG GTTGTAGTGG TGCCCAATGA GCCGTCTTTC 
CACCAGCGGC GGCCCTACAC GAGCGAGGAT GAGGCCTGGA GGTCTTACCT GGAGAATCCA 
CTTACTGCGG CCACAAAGGC TATGATGAGC ATCAATGGAG ATGAAGACAG TGCTGCCGCC 
CTGGGGCTCC TTTATGAGTA CTACAAGGTG CCCAAAGATA AGAGAGTACC TATCAGCAAA 
GTGGTTGAAA TGTCAGACGA ACCGGAAAAG AGAAGCACAC CAGTGGGAAA CAGCAGCCAA 
ATGGAGCCGG AGACGGTGGA TAATCATGTC CAAGTCCTGA AATCAGTGCC TGTTAATCTG 
TCACTCAACA CGGATCACCA GGACAATAAA CGGGAACAGT ACAGCGTGCC GGCTGGAGAG 
AGCGGGACGG CTGCTGTGGT AAAAGCTGAA GCGCACACTG CTGTCTTTAT GACTTCAGGG 
GGACACTACA GGACAGAAGG GGAGGAGCAG ATGCGGGTCA TCTGTGAACA GACCCACTCA 
TTCGACACCT CCACCATAAA TCACAGTGCA TATGGAAAAG ATGACCATCG CAGTAGTCCA 
GAAAGCACAT ATGAAGATGA CAGCAATGAA AAGTACCGCT CGTCTTCGAT TGGACCTGAT 
GACTTCATTT TTGACCAGTC ACATATGTTA GACACATTTC AATACACACT AGAGGCCAGC 
AAGTCATTGC GGCAAAAGCA GGGCGAGGGG CCTATGACTT ATCTGAACAA GGGCCAGTTC 
TATGCTATTA CACTAAGTGA GACAAGTGCT AATAAACGCC TTCGACATCC CATCAGCAAA 
GTGAGGAGTG TCGTCATGGT TGTATTCAGT GAGGATAAGA ATCGAGATGA GCAACTTAAA 
TACTGGAAGT ATTGGCACTC CCGCCAGCAC ACGGCGAAGC AGAGGGTTCT GGACATTGCT 
GACTACAAGG AGAGCTTCAA CACAATTGGA AATATTGAAG AAATTGCATA CAACGCTGTG 
TCTTTCACCT GGGACCTGAA CGAGGAGGCC AAGATCTTCA TCACGGTGAA CTGTCTAAGC 
ACAGATTTCT CCTCTCAGAA GGGGGTGAAA GGTCTTCCTC TGATGATACA GATTGACACC 
TACAGCTACA ACAACCGCAG TAACAAACCC CTGCACAGAG CCTACTGCCA GATCAAGGTC 
TTCTGTGACA AGGGAGCGGA GAGGAAGATC AGAGATGAGG AGAGGAAACA AAACCGCAAG 
AAGACAAAAG GAAAGGATGG AGGCAGCGGG ACCGTTACTG TCCCGAAAAA ATGTGACAGC 
ACCCTCTTCA AAACGATGAC TGATTTGGAT GCCCAGCCAG TTCTCTTTAT CCCGGATGTG 
CACTTTGGGA ACCTACAGAG GGCAGGCCAG GTATTTGCAT TCAATACAGA GGAAATTGAA 
TGTGAGGGGA GTGTGCTGGT GAAGAGGATG TTCCGGTCAT CTGAGGAAGA CTTCTGCCCA 
CCGCCACATA AACAGCTCAG AGAAGACACA CAGAAGAGAG TGCTGTTGTA CGTGAGGAAG 
GAGTCAGACG AGGTGTTTGA TGCTCTGATG TTGCGGTCGC CCACTGTCAG AGGACTCATG 
GATGCAATTT CTGAGAAGTA TGGTGTTCCC ATTGACAGAA TGGCTAAGAT TTACAAAAAG 
AGCAAGAAAG GGATTCTGGT GAACATGGAC GACAACATCA TCGAGCACTA CTCAAACGAG 
GACACGTTTA TCCTGAATAT CGAGAGCTAC GCAGACGCAT ACAAAATCAC TTTAACGGAA 
ATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

grhl2b ( XM_005159580.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159637.1 Danio rerio grainyhead-like transcription factor 2b (grhl2b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa23993
Clone ID Related Accession (Same CDS sequence)	XM_005159580.3 , XM_005159580.4
Accession Version	XM_005159580.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1806bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	grainyhead-like protein 2 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159580.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACAGG AGACAGACAG TAAGAGGCTG GTTGTAGTGG TGCCCAATGA GCCGTCTTTC 
CACCAGCGGC GGCCCTACAC GAGCGAGGAT GAGGCCTGGA GGTCTTACCT GGAGAATCCA 
CTTACTGCGG CCACAAAGGC TATGATGAGC ATCAATGGAG ATGAAGACAG TGCTGCCGCC 
CTGGGGCTCC TTTATGAGTA CTACAAGGTG CCCAAAGATA AGAGAGTACC TATCAGCAAA 
GTGGTTGAAA TGTCAGACGA ACCGGAAAAG AGAAGCACAC CAGTGGGAAA CAGCAGCCAA 
ATGGAGCCGG AGACGGTGGA TAATCATGTC CAAGTCCTGA AATCAGTGCC TGTTAATCTG 
TCACTCAACA CGGATCACCA GGACAATAAA CGGGAACAGT ACAGCGTGCC GGCTGGAGAG 
AGCGGGACGG CTGCTGTGGT AAAAGCTGAA GCGCACACTG CTGTCTTTAT GACTTCAGGG 
GGACACTACA GGACAGAAGG GGAGGAGCAG ATGCGGGTCA TCTGTGAACA GACCCACTCA 
TTCGACACCT CCACCATAAA TCACAGTGCA TATGGAAAAG ATGACCATCG CAGTAGTCCA 
GAAAGCACAT ATGAAGATGA CAGCAATGAA AAGTACCGCT CGTCTTCGAT TGGACCTGAT 
GACTTCATTT TTGACCAGTC ACATATGTTA GACACATTTC AATACACACT AGAGGCCAGC 
AAGTCATTGC GGCAAAAGCA GGGCGAGGGG CCTATGACTT ATCTGAACAA GGGCCAGTTC 
TATGCTATTA CACTAAGTGA GACAAGTGCT AATAAACGCC TTCGACATCC CATCAGCAAA 
GTGAGGAGTG TCGTCATGGT TGTATTCAGT GAGGATAAGA ATCGAGATGA GCAACTTAAA 
TACTGGAAGT ATTGGCACTC CCGCCAGCAC ACGGCGAAGC AGAGGGTTCT GGACATTGCT 
GACTACAAGG AGAGCTTCAA CACAATTGGA AATATTGAAG AAATTGCATA CAACGCTGTG 
TCTTTCACCT GGGACCTGAA CGAGGAGGCC AAGATCTTCA TCACGGTGAA CTGTCTAAGC 
ACAGATTTCT CCTCTCAGAA GGGGGTGAAA GGTCTTCCTC TGATGATACA GATTGACACC 
TACAGCTACA ACAACCGCAG TAACAAACCC CTGCACAGAG CCTACTGCCA GATCAAGGTC 
TTCTGTGACA AGGGAGCGGA GAGGAAGATC AGAGATGAGG AGAGGAAACA AAACCGCAAG 
AAGACAAAAG GAAAGGATGG AGGCAGCGGG ACCGTTACTG TCCCGAAAAA ATGTGACAGC 
ACCCTCTTCA AAACGATGAC TGATTTGGAT GCCCAGCCAG TTCTCTTTAT CCCGGATGTG 
CACTTTGGGA ACCTACAGAG GGCAGGCCAG GTATTTGCAT TCAATACAGA GGAAATTGAA 
TGTGAGGGGA GTGTGCTGGT GAAGAGGATG TTCCGGTCAT CTGAGGAAGA CTTCTGCCCA 
CCGCCACATA AACAGCTCAG AGAAGACACA CAGAAGAGAG TGCTGTTGTA CGTGAGGAAG 
GAGTCAGACG AGGTGTTTGA TGCTCTGATG TTGCGGTCGC CCACTGTCAG AGGACTCATG 
GATGCAATTT CTGAGAAGTA TGGTGTTCCC ATTGACAGAA TGGCTAAGAT TTACAAAAAG 
AGCAAGAAAG GGATTCTGGT GAACATGGAC GACAACATCA TCGAGCACTA CTCAAACGAG 
GACACGTTTA TCCTGAATAT CGAGAGCTAC GCAGACGCAT ACAAAATCAC TTTAACGGAA 
ATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159637.1
CDS	278..2083
Translation	MSQETDSKRLVVVVPNEPSFHQRRPYTSEDEAWRSYLENPLTAATKAMMSINGDEDSAAALGLLYEYYKVPKDKRVPISKVVEMSDEPEKRSTPVGNSSQMEPETVDNHVQVLKSVPVNLSLNTDHQDNKREQYSVPAGESGTAAVVKAEAHTAVFMTSGGHYRTEGEEQMRVICEQTHSFDTSTINHSAYGKDDHRSSPESTYEDDSNEKYRSSSIGPDDFIFDQSHMLDTFQYTLEASKSLRQKQGEGPMTYLNKGQFYAITLSETSANKRLRHPISKVRSVVMVVFSEDKNRDEQLKYWKYWHSRQHTAKQRVLDIADYKESFNTIGNIEEIAYNAVSFTWDLNEEAKIFITVNCLSTDFSSQKGVKGLPLMIQIDTYSYNNRSNKPLHRAYCQIKVFCDKGAERKIRDEERKQNRKKTKGKDGGSGTVTVPKKCDSTLFKTMTDLDAQPVLFIPDVHFGNLQRAGQVFAFNTEEIECEGSVLVKRMFRSSEEDFCPPPHKQLREDTQKRVLLYVRKESDEVFDALMLRSPTVRGLMDAISEKYGVPIDRMAKIYKKSKKGILVNMDDNIIEHYSNEDTFILNIESYADAYKITLTEI

Target ORF information:

RefSeq Version	XM_005159580.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio grainyhead-like transcription factor 2b (grhl2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159580.4

ORF Insert Sequence:

ATGTCACAGG AGACAGACAG TAAGAGGCTG GTTGTAGTGG TGCCCAATGA GCCGTCTTTC 
CACCAGCGGC GGCCCTACAC GAGCGAGGAT GAGGCCTGGA GGTCTTACCT GGAGAATCCA 
CTTACTGCGG CCACAAAGGC TATGATGAGC ATCAATGGAG ATGAAGACAG TGCTGCCGCC 
CTGGGGCTCC TTTATGAGTA CTACAAGGTG CCCAAAGATA AGAGAGTACC TATCAGCAAA 
GTGGTTGAAA TGTCAGACGA ACCGGAAAAG AGAAGCACAC CAGTGGGAAA CAGCAGCCAA 
ATGGAGCCGG AGACGGTGGA TAATCATGTC CAAGTCCTGA AATCAGTGCC TGTTAATCTG 
TCACTCAACA CGGATCACCA GGACAATAAA CGGGAACAGT ACAGCGTGCC GGCTGGAGAG 
AGCGGGACGG CTGCTGTGGT AAAAGCTGAA GCGCACACTG CTGTCTTTAT GACTTCAGGG 
GGACACTACA GGACAGAAGG GGAGGAGCAG ATGCGGGTCA TCTGTGAACA GACCCACTCA 
TTCGACACCT CCACCATAAA TCACAGTGCA TATGGAAAAG ATGACCATCG CAGTAGTCCA 
GAAAGCACAT ATGAAGATGA CAGCAATGAA AAGTACCGCT CGTCTTCGAT TGGACCTGAT 
GACTTCATTT TTGACCAGTC ACATATGTTA GACACATTTC AATACACACT AGAGGCCAGC 
AAGTCATTGC GGCAAAAGCA GGGCGAGGGG CCTATGACTT ATCTGAACAA GGGCCAGTTC 
TATGCTATTA CACTAAGTGA GACAAGTGCT AATAAACGCC TTCGACATCC CATCAGCAAA 
GTGAGGAGTG TCGTCATGGT TGTATTCAGT GAGGATAAGA ATCGAGATGA GCAACTTAAA 
TACTGGAAGT ATTGGCACTC CCGCCAGCAC ACGGCGAAGC AGAGGGTTCT GGACATTGCT 
GACTACAAGG AGAGCTTCAA CACAATTGGA AATATTGAAG AAATTGCATA CAACGCTGTG 
TCTTTCACCT GGGACCTGAA CGAGGAGGCC AAGATCTTCA TCACGGTGAA CTGTCTAAGC 
ACAGATTTCT CCTCTCAGAA GGGGGTGAAA GGTCTTCCTC TGATGATACA GATTGACACC 
TACAGCTACA ACAACCGCAG TAACAAACCC CTGCACAGAG CCTACTGCCA GATCAAGGTC 
TTCTGTGACA AGGGAGCGGA GAGGAAGATC AGAGATGAGG AGAGGAAACA AAACCGCAAG 
AAGACAAAAG GAAAGGATGG AGGCAGCGGG ACCGTTACTG TCCCGAAAAA ATGTGACAGC 
ACCCTCTTCA AAACGATGAC TGATTTGGAT GCCCAGCCAG TTCTCTTTAT CCCGGATGTG 
CACTTTGGGA ACCTACAGAG GGCAGGCCAG GTATTTGCAT TCAATACAGA GGAAATTGAA 
TGTGAGGGGA GTGTGCTGGT GAAGAGGATG TTCCGGTCAT CTGAGGAAGA CTTCTGCCCA 
CCGCCACATA AACAGCTCAG AGAAGACACA CAGAAGAGAG TGCTGTTGTA CGTGAGGAAG 
GAGTCAGACG AGGTGTTTGA TGCTCTGATG TTGCGGTCGC CCACTGTCAG AGGACTCATG 
GATGCAATTT CTGAGAAGTA TGGTGTTCCC ATTGACAGAA TGGCTAAGAT TTACAAAAAG 
AGCAAGAAAG GGATTCTGGT GAACATGGAC GACAACATCA TCGAGCACTA CTCAAACGAG 
GACACGTTTA TCCTGAATAT CGAGAGCTAC GCAGACGCAT ACAAAATCAC TTTAACGGAA 
ATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

grhl2b ( XM_009294097.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292372.1 Danio rerio grainyhead-like transcription factor 2b (grhl2b), transcript variant X2, mRNA.

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CloneID	ODa48381
Clone ID Related Accession (Same CDS sequence)	XM_009294097.2
Accession Version	XM_009294097.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	grainyhead-like protein 2 homolog isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294097.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACAGG AGACAGACAG TAAGAGGCTG GTTGTAGTGG TGCCCAATGA GCCGTCTTTC 
CACCAGCGGC GGCCCTACAC GAGCGAGGAT GAGGCCTGGA GGTCTTACCT GGAGAATCCA 
CTTACTGCGG CCACAAAGGC TATGATGAGC ATCAATGGAG ATGAAGACAG TGCTGCCGCC 
CTGGGGCTCC TTTATGAGTA CTACAAGGTG CCCAAAGATA AGAGAGTACC TATCAGCAAA 
GTGGTTGAAA TGTCAGACGA ACCGGAAAAG AGAAGCACAC CAGTGGGAAA CAGCAGCCAA 
ATGGAGCCGG AGACGGTGGA TAATCATGTC CAAGTCCTGA AATCAGTGCC TGTTAATCTG 
TCACTCAACA CGGATCACCA GGACAATAAA CGGGAACAGT ACAGCGTGCC GGCTGGAGAG 
AGCGGGACGG CTGCTGTGGT AAAAGCTGAA GCGCACACTG CTGTCTTTAT GACTTCAGGG 
GGACACTACA GGACAGAAGG GGAGGAGCAG ATGCGGGTCA TCTGTGAACA GACCCACTCA 
TTCGACACCT CCACCATAAA TCACAGTGCA TATGGAAAAG ATGACCATCG CAGTAGTCCA 
GAAAGCACAT ATGAAGATGA CAGCAATGAA AAGTACCGCT CGTCTTCGAT TGGACCTGAT 
GACTTCATTT TTGACCAGTC ACATATGTTA GACACATTTC AATACACACT AGAGGCCAGC 
AAGTCATTGC GGCAAAAGCA GGGCGAGGGG CCTATGACTT ATCTGAACAA GGGCCAGTTC 
TATGCTATTA CACTAAGTGA GACAAGTGCT AATAAACGCC TTCGACATCC CATCAGCAAA 
GTGAGGAGTG TCGTCATGGT TGTATTCAGT GAGGATAAGA ATCGAGATGA GCAACTTAAA 
TACTGGAAGT ATTGGCACTC CCGCCAGCAC ACGGCGAAGC AGAGGGTTCT GGACATTGGC 
ATGGGCCGGT ATACGATTCT GACGGTGTGG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292372.1
CDS	283..1275
Translation	MSQETDSKRLVVVVPNEPSFHQRRPYTSEDEAWRSYLENPLTAATKAMMSINGDEDSAAALGLLYEYYKVPKDKRVPISKVVEMSDEPEKRSTPVGNSSQMEPETVDNHVQVLKSVPVNLSLNTDHQDNKREQYSVPAGESGTAAVVKAEAHTAVFMTSGGHYRTEGEEQMRVICEQTHSFDTSTINHSAYGKDDHRSSPESTYEDDSNEKYRSSSIGPDDFIFDQSHMLDTFQYTLEASKSLRQKQGEGPMTYLNKGQFYAITLSETSANKRLRHPISKVRSVVMVVFSEDKNRDEQLKYWKYWHSRQHTAKQRVLDIGMGRYTILTVW

Target ORF information:

RefSeq Version	XM_009294097.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio grainyhead-like transcription factor 2b (grhl2b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294097.2

ORF Insert Sequence:

ATGTCACAGG AGACAGACAG TAAGAGGCTG GTTGTAGTGG TGCCCAATGA GCCGTCTTTC 
CACCAGCGGC GGCCCTACAC GAGCGAGGAT GAGGCCTGGA GGTCTTACCT GGAGAATCCA 
CTTACTGCGG CCACAAAGGC TATGATGAGC ATCAATGGAG ATGAAGACAG TGCTGCCGCC 
CTGGGGCTCC TTTATGAGTA CTACAAGGTG CCCAAAGATA AGAGAGTACC TATCAGCAAA 
GTGGTTGAAA TGTCAGACGA ACCGGAAAAG AGAAGCACAC CAGTGGGAAA CAGCAGCCAA 
ATGGAGCCGG AGACGGTGGA TAATCATGTC CAAGTCCTGA AATCAGTGCC TGTTAATCTG 
TCACTCAACA CGGATCACCA GGACAATAAA CGGGAACAGT ACAGCGTGCC GGCTGGAGAG 
AGCGGGACGG CTGCTGTGGT AAAAGCTGAA GCGCACACTG CTGTCTTTAT GACTTCAGGG 
GGACACTACA GGACAGAAGG GGAGGAGCAG ATGCGGGTCA TCTGTGAACA GACCCACTCA 
TTCGACACCT CCACCATAAA TCACAGTGCA TATGGAAAAG ATGACCATCG CAGTAGTCCA 
GAAAGCACAT ATGAAGATGA CAGCAATGAA AAGTACCGCT CGTCTTCGAT TGGACCTGAT 
GACTTCATTT TTGACCAGTC ACATATGTTA GACACATTTC AATACACACT AGAGGCCAGC 
AAGTCATTGC GGCAAAAGCA GGGCGAGGGG CCTATGACTT ATCTGAACAA GGGCCAGTTC 
TATGCTATTA CACTAAGTGA GACAAGTGCT AATAAACGCC TTCGACATCC CATCAGCAAA 
GTGAGGAGTG TCGTCATGGT TGTATTCAGT GAGGATAAGA ATCGAGATGA GCAACTTAAA 
TACTGGAAGT ATTGGCACTC CCGCCAGCAC ACGGCGAAGC AGAGGGTTCT GGACATTGGC 
ATGGGCCGGT ATACGATTCT GACGGTGTGG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mis-expression of grainyhead-like transcription factors in zebrafish leads to defects in enveloping layer (EVL) integrity, cellular morphogenesis and axial extension.
Scientific reports7(1)17607(2017 Dec)
Miles LB,Darido C,Kaslin J,Heath JK,Jane SM,Dworkin S

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

High-throughput gene targeting and phenotyping in zebrafish using CRISPR/Cas9.
Genome research25(7)1030-42(2015 Jul)
Varshney GK,Pei W,LaFave MC,Idol J,Xu L,Gallardo V,Carrington B,Bishop K,Jones M,Li M,Harper U,Huang SC,Prakash A,Chen W,Sood R,Ledin J,Burgess SM

Grhl1 deficiency affects inner ear development in zebrafish.
The International journal of developmental biology59(10-12)417-23(2015)
Liu F,Yang F,Wen D,Xia W,Hao L,Hu J,Zong J,Shen X,Ma J,Jiang N,Sun S,Zhang J,Wang H,Wang X,Ma Z,Ma D

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grhl2b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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