pkn1b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	pkn1b
Entrez Gene ID	100007897
Full Name	protein kinase N1b
Synonyms	zgc:162290
General protein information	Preferred Names protein kinase N1b Names protein kinase N1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001089460.1	NP_001082929.1	protein kinase N1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PKN1	XP_512443.3
Danio rerio (zebrafish)	LOC560840	XP_005160108.1
Homo sapiens (human)	PKN1	NP_002732.3
Canis lupus familiaris (dog)	PKN1	XP_003639827.1
Bos taurus (cattle)	PKN1	NP_001073715.1
Rattus norvegicus (Norway rat)	Pkn1	NP_058871.2
Danio rerio (zebrafish)	zgc:162290	NP_001082929.1
	LOC718834	XP_002808221.1
Mus musculus (house mouse)	Pkn1	NP_796236.2

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Comparative analysis of the human and zebrafish kinomes: focus on the development of kinase inhibitors.
Wlodarchak N, Tariq R, Striker R
Trends in cell & molecular biology1049-75(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following pkn1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pkn1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa00907	NM_001089460.1 Latest version!	Danio rerio protein kinase N1b (pkn1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pkn1b ( NM_001089460.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001082929.1 Danio rerio protein kinase N1b (pkn1b), mRNA.

Price & Availability↑

CloneID	ODa00907
Clone ID Related Accession (Same CDS sequence)	NM_001089460.1
Accession Version	NM_001089460.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	protein kinase N1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC139563.1. On May 24, 2007 this sequence version replaced XM_001346204.1. ##Evidence-Data-START## Transcript exon combination :: BC139563.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGGACTGG ACCAGAACTC TGATTTCTCC GACTCCAGCG TGCAGCATCG GCTGGACGAG 
AAGCGCGAGT GGATCCGCAG GGAGATCCGT AAAGAACTGA AGATTAAAGA GGGCGCGGAG 
AAACTGCGGC GGGCCACTAC TGACAAACGC AATGCCCAGC AGGTGGAGAG CCAGTTGCGC 
AGCTCCAGCC GAAAGCTGGA CTCGCTCCAC GCTCAGCTGC AGGAGCTGGA CGCATATATC 
ATGGTCAAGG GTCACGACGA CAGCAAAGAT GACATTCAGT CTGTTGGGAC ACCAGGACGC 
TCCTCAGCCA ATCAGGACAG GATTAAGGCT CTGGAGCGAC AGCTCAACAT CGAACTGAAG 
GTCAAGCAGG GGGCAGAGAA CATGATCCCC ATCTACGCCA ATGGGGTTAC CAAGGATAAG 
AAGCTGCTTC AGTCGGCTCA GCAGATGCTG CAGGACAGTA AAACGAAGAT CGACATCATC 
CGAATGCAGA TCCGTAAAGC CATGCAGGCC ACTGAACAGA GCGACGACAC TCAGGGTAAG 
CCGGATCTGT GTGGTGTGGA GCTGCGTATC GAGGAGCTCT GGCATCATTA CCGTGTTGAA 
CACGCCATGG CTGAAGGCGC CAAAAACATG CTGAGGCTTC TGGGAACTGG AAAAGCGCAG 
GACAAGAAAG CCATCGCTGA GGCTCAGAGT CGTTTGAGTG AAGCGTCCCA GAGGCTGGAT 
CTCCTGCGGG TTTCCTTAGA GCAGCGTTTG GTGAATCTGC CCGAGGATCA TCCCAAAGCG 
TGTCTGATTA AAGAGGAGCT GGTGCGGGCG TCCTCCTCCG CCTTCAGCTC CAGTCACAGC 
GCCCCCTATG TCCACAATCA GTACAGCACC CTCAGCAGAC CCTCCCCGCT CACCGGCACT 
CTGCAGGTGC GTCTGCTGGG CTGTGTGGAT TTACTGGAGA TGGTCCCGGG CCGCAGTCGA 
GCGTCTCCCG TGGTTTTACC TCACTTCAGT CCTGGAGACG GACGAGCCTT CAAGCTGACC 
CTGAGCAGCA GAAGTAGCAG CTTCAGCCTG AAGACGCCCA GCAAGAGTGA AGAGTCCTCG 
GAGGTGAGCG CTGTGCTGAA GCTGGAGAAC TGTGTTGTGG GTCAGACGTG TTGGAAGAGT 
GTTGGGGAAC AGACGTGGGA TCAGACCTTC ACTCTGGAGC TGGAGAGATC TAGAGAGATG 
GAGATAGCGC TGTACTGGAA GGATTATCGC TCGCTCTGTG CGCTGAAGTA TCTGAAACTG 
GAGGATTTTC TGGACAATCA GAGACACAGA GTTCAGCTGG AGCTGGAGCC GCAGGGTCTG 
CTGTTGGCCG AGGTGATGTT CTTCAATCCT GTGATCGAGC GAGGGCCACG TTTACAGCGC 
CAGAGGAAGG TGTTTTCTAA ACAACAGGGT AAAGCGTTTC TGCGTGCTAA ACAGATGAAT 
GTGGACATGG CGACATGGGT CCGTCTGCTG AAGAACGCCA TTACCATCCC GACACACAAC 
TACACACAGC ACAGCCACAC ACACCACAGC AGCAGTGAGA TCTCCATCCA GAAGATCCAG 
CTTGATGGAG AATCTCCTCC ATCTGTGACG AGGAGAAACA CACACACGTC AGCCACGGTG 
GACTTCGGCC GGGCGGCTGA ACCCGTCTCT CCTCCTGGTC CCGCGCCTCC GGAGAGGGTC 
GTCCGGAGCA TTTCGCATCT TTCCCAGCGC AGGTCATCCA AAAGCGCTCT GTGTCTTCAG 
GATTTCCGAA TGATTGCAGT GTTGGGAAGG GGACATTTTG GAAAGGTACT CCTGTCTGAA 
TACAGACGCT CGGGAAATCT GTACGCCATC AAAGCACTGA AGAAAGGAGA CATCGTTGCA 
CGGGATGAGG TGGAGAGTTT GATGTGTGAG AAGCGAATCT TCGAGACGGT GAACAGCGCA 
CAGCACCCGT TCCTGGTCAA CCTGTTTGCG TGCTTTCAGA CGCCAGAACA CGTGTGTTTC 
GTGATGGAGT ACACGGCCGG CGGAGACCTG ATGATGCACA TACATGCAGA CGTCTTCTCT 
GAAACACGCT CTGTGTTTTA TTCTGCATGT GTAGTTTTGG GGCTTCAGTT TCTTCATGAT 
AATAAGATTG TGTATCGAGA TCTGAAGCTG GACAACCTGC TGCTGGATAC TGAAGGTTAT 
GTGAAAATCG CAGACTTCGG CCTCTGTAAA GAGGGAATGG GTCACGGGGA CCGCACCAGC 
ACTTTCTGTG GGACTCCAGA GTTTCTGGCT CCTGAGGTTT TGACGGACAC ATCCTACACG 
CGGGCCGTGG ACTGGTGGGG TCTCGGAGTG CTCATTTATG AGATGATGGT CGGAGAGTCT 
CCCTTCCCAG GTGACGATGA GGAGGAGGTG TTCGACAGCA TCGTGAATGA TGAAGTTCGA 
TACCCGAGAT TCCTCTCCAC TGAAGCCATT GGCATCATGA GACGGCTGCT GAGGCGTAAT 
CCTGAGAGAC GGCTGGGCTC CGGCGAGAAA GACGCAGAGG ACATAAAGAA ACAGCCCTTC 
TTTAGGAATA TGGACTGGGA TGCTCTTCTG CAGCGAAAGG TTCCTCCTCC ATTTGTTCCA 
GCAGTCGCTA ACAGTGAAGA TGTCAGTAAT TTCGACGCAG AGTTTACGAA CGAGGCTCCA 
ACACTGACGC CGCCTCGAGA ACGCCGGAGC CTTTCACGCA AAGACCAGGA CTATTTTAAG 
GACTTTGATT ACGTCTCTGA CTTCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001082929.1
CDS	115..2844
Translation	MGLDQNSDFSDSSVQHRLDEKREWIRREIRKELKIKEGAEKLRRATTDKRNAQQVESQLRSSSRKLDSLHAQLQELDAYIMVKGHDDSKDDIQSVGTPGRSSANQDRIKALERQLNIELKVKQGAENMIPIYANGVTKDKKLLQSAQQMLQDSKTKIDIIRMQIRKAMQATEQSDDTQGKPDLCGVELRIEELWHHYRVEHAMAEGAKNMLRLLGTGKAQDKKAIAEAQSRLSEASQRLDLLRVSLEQRLVNLPEDHPKACLIKEELVRASSSAFSSSHSAPYVHNQYSTLSRPSPLTGTLQVRLLGCVDLLEMVPGRSRASPVVLPHFSPGDGRAFKLTLSSRSSSFSLKTPSKSEESSEVSAVLKLENCVVGQTCWKSVGEQTWDQTFTLELERSREMEIALYWKDYRSLCALKYLKLEDFLDNQRHRVQLELEPQGLLLAEVMFFNPVIERGPRLQRQRKVFSKQQGKAFLRAKQMNVDMATWVRLLKNAITIPTHNYTQHSHTHHSSSEISIQKIQLDGESPPSVTRRNTHTSATVDFGRAAEPVSPPGPAPPERVVRSISHLSQRRSSKSALCLQDFRMIAVLGRGHFGKVLLSEYRRSGNLYAIKALKKGDIVARDEVESLMCEKRIFETVNSAQHPFLVNLFACFQTPEHVCFVMEYTAGGDLMMHIHADVFSETRSVFYSACVVLGLQFLHDNKIVYRDLKLDNLLLDTEGYVKIADFGLCKEGMGHGDRTSTFCGTPEFLAPEVLTDTSYTRAVDWWGLGVLIYEMMVGESPFPGDDEEEVFDSIVNDEVRYPRFLSTEAIGIMRRLLRRNPERRLGSGEKDAEDIKKQPFFRNMDWDALLQRKVPPPFVPAVANSEDVSNFDAEFTNEAPTLTPPRERRSLSRKDQDYFKDFDYVSDFC

Target ORF information:

RefSeq Version	NM_001089460.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein kinase N1b (pkn1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001089460.1

ORF Insert Sequence:

ATGGGACTGG ACCAGAACTC TGATTTCTCC GACTCCAGCG TGCAGCATCG GCTGGACGAG 
AAGCGCGAGT GGATCCGCAG GGAGATCCGT AAAGAACTGA AGATTAAAGA GGGCGCGGAG 
AAACTGCGGC GGGCCACTAC TGACAAACGC AATGCCCAGC AGGTGGAGAG CCAGTTGCGC 
AGCTCCAGCC GAAAGCTGGA CTCGCTCCAC GCTCAGCTGC AGGAGCTGGA CGCATATATC 
ATGGTCAAGG GTCACGACGA CAGCAAAGAT GACATTCAGT CTGTTGGGAC ACCAGGACGC 
TCCTCAGCCA ATCAGGACAG GATTAAGGCT CTGGAGCGAC AGCTCAACAT CGAACTGAAG 
GTCAAGCAGG GGGCAGAGAA CATGATCCCC ATCTACGCCA ATGGGGTTAC CAAGGATAAG 
AAGCTGCTTC AGTCGGCTCA GCAGATGCTG CAGGACAGTA AAACGAAGAT CGACATCATC 
CGAATGCAGA TCCGTAAAGC CATGCAGGCC ACTGAACAGA GCGACGACAC TCAGGGTAAG 
CCGGATCTGT GTGGTGTGGA GCTGCGTATC GAGGAGCTCT GGCATCATTA CCGTGTTGAA 
CACGCCATGG CTGAAGGCGC CAAAAACATG CTGAGGCTTC TGGGAACTGG AAAAGCGCAG 
GACAAGAAAG CCATCGCTGA GGCTCAGAGT CGTTTGAGTG AAGCGTCCCA GAGGCTGGAT 
CTCCTGCGGG TTTCCTTAGA GCAGCGTTTG GTGAATCTGC CCGAGGATCA TCCCAAAGCG 
TGTCTGATTA AAGAGGAGCT GGTGCGGGCG TCCTCCTCCG CCTTCAGCTC CAGTCACAGC 
GCCCCCTATG TCCACAATCA GTACAGCACC CTCAGCAGAC CCTCCCCGCT CACCGGCACT 
CTGCAGGTGC GTCTGCTGGG CTGTGTGGAT TTACTGGAGA TGGTCCCGGG CCGCAGTCGA 
GCGTCTCCCG TGGTTTTACC TCACTTCAGT CCTGGAGACG GACGAGCCTT CAAGCTGACC 
CTGAGCAGCA GAAGTAGCAG CTTCAGCCTG AAGACGCCCA GCAAGAGTGA AGAGTCCTCG 
GAGGTGAGCG CTGTGCTGAA GCTGGAGAAC TGTGTTGTGG GTCAGACGTG TTGGAAGAGT 
GTTGGGGAAC AGACGTGGGA TCAGACCTTC ACTCTGGAGC TGGAGAGATC TAGAGAGATG 
GAGATAGCGC TGTACTGGAA GGATTATCGC TCGCTCTGTG CGCTGAAGTA TCTGAAACTG 
GAGGATTTTC TGGACAATCA GAGACACAGA GTTCAGCTGG AGCTGGAGCC GCAGGGTCTG 
CTGTTGGCCG AGGTGATGTT CTTCAATCCT GTGATCGAGC GAGGGCCACG TTTACAGCGC 
CAGAGGAAGG TGTTTTCTAA ACAACAGGGT AAAGCGTTTC TGCGTGCTAA ACAGATGAAT 
GTGGACATGG CGACATGGGT CCGTCTGCTG AAGAACGCCA TTACCATCCC GACACACAAC 
TACACACAGC ACAGCCACAC ACACCACAGC AGCAGTGAGA TCTCCATCCA GAAGATCCAG 
CTTGATGGAG AATCTCCTCC ATCTGTGACG AGGAGAAACA CACACACGTC AGCCACGGTG 
GACTTCGGCC GGGCGGCTGA ACCCGTCTCT CCTCCTGGTC CCGCGCCTCC GGAGAGGGTC 
GTCCGGAGCA TTTCGCATCT TTCCCAGCGC AGGTCATCCA AAAGCGCTCT GTGTCTTCAG 
GATTTCCGAA TGATTGCAGT GTTGGGAAGG GGACATTTTG GAAAGGTACT CCTGTCTGAA 
TACAGACGCT CGGGAAATCT GTACGCCATC AAAGCACTGA AGAAAGGAGA CATCGTTGCA 
CGGGATGAGG TGGAGAGTTT GATGTGTGAG AAGCGAATCT TCGAGACGGT GAACAGCGCA 
CAGCACCCGT TCCTGGTCAA CCTGTTTGCG TGCTTTCAGA CGCCAGAACA CGTGTGTTTC 
GTGATGGAGT ACACGGCCGG CGGAGACCTG ATGATGCACA TACATGCAGA CGTCTTCTCT 
GAAACACGCT CTGTGTTTTA TTCTGCATGT GTAGTTTTGG GGCTTCAGTT TCTTCATGAT 
AATAAGATTG TGTATCGAGA TCTGAAGCTG GACAACCTGC TGCTGGATAC TGAAGGTTAT 
GTGAAAATCG CAGACTTCGG CCTCTGTAAA GAGGGAATGG GTCACGGGGA CCGCACCAGC 
ACTTTCTGTG GGACTCCAGA GTTTCTGGCT CCTGAGGTTT TGACGGACAC ATCCTACACG 
CGGGCCGTGG ACTGGTGGGG TCTCGGAGTG CTCATTTATG AGATGATGGT CGGAGAGTCT 
CCCTTCCCAG GTGACGATGA GGAGGAGGTG TTCGACAGCA TCGTGAATGA TGAAGTTCGA 
TACCCGAGAT TCCTCTCCAC TGAAGCCATT GGCATCATGA GACGGCTGCT GAGGCGTAAT 
CCTGAGAGAC GGCTGGGCTC CGGCGAGAAA GACGCAGAGG ACATAAAGAA ACAGCCCTTC 
TTTAGGAATA TGGACTGGGA TGCTCTTCTG CAGCGAAAGG TTCCTCCTCC ATTTGTTCCA 
GCAGTCGCTA ACAGTGAAGA TGTCAGTAAT TTCGACGCAG AGTTTACGAA CGAGGCTCCA 
ACACTGACGC CGCCTCGAGA ACGCCGGAGC CTTTCACGCA AAGACCAGGA CTATTTTAAG 
GACTTTGATT ACGTCTCTGA CTTCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Comparative analysis of the human and zebrafish kinomes: focus on the development of kinase inhibitors.
Trends in cell & molecular biology1049-75(2015)
Wlodarchak N,Tariq R,Striker R

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pkn1b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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