LOC100007608 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100007608
Entrez Gene ID	100007608
Full Name	calmodulin-regulated spectrin-associated protein 3-like
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005171060.3	XP_005171117.1	calmodulin-regulated spectrin-associated protein 3-like isoform X2
XM_005171061.3	XP_005171118.1	calmodulin-regulated spectrin-associated protein 3-like isoform X3
XM_003197797.4	XP_003197845.1	calmodulin-regulated spectrin-associated protein 3-like isoform X1
XM_003197797.5	XP_003197845.1	calmodulin-regulated spectrin-associated protein 3-like isoform X1
XM_005171060.4	XP_005171117.1	calmodulin-regulated spectrin-associated protein 3-like isoform X2
XM_005171061.4	XP_005171118.1	calmodulin-regulated spectrin-associated protein 3-like isoform X3

Pathways from BioSystems
Gene Ontology

The following LOC100007608 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100007608 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa06526	XM_003197797.5 Latest version!	Danio rerio calmodulin-regulated spectrin-associated protein 3-like (LOC100007608), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa06527	XM_005171060.4 Latest version!	Danio rerio calmodulin-regulated spectrin-associated protein 3-like (LOC100007608), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa06528	XM_005171061.4 Latest version!	Danio rerio calmodulin-regulated spectrin-associated protein 3-like (LOC100007608), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
ODa06526	XM_003197797.4	Danio rerio calmodulin-regulated spectrin-associated protein 3-like (LOC100007608), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa06527	XM_005171060.3	Danio rerio calmodulin-regulated spectrin-associated protein 3-like (LOC100007608), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa06528	XM_005171061.3	Danio rerio calmodulin-regulated spectrin-associated protein 3-like (LOC100007608), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100007608 ( XM_003197797.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_003197845.1 Danio rerio calmodulin-regulated spectrin-associated protein 3-like (LOC100007608), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa06526
Clone ID Related Accession (Same CDS sequence)	XM_003197797.4 , XM_003197797.5
Accession Version	XM_003197797.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	calmodulin-regulated spectrin-associated protein 3-like isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_003197797.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGATGG ACGCGCCCGC GGTGAAGCAG AGCGCGCCGG TACCGGACAT CTGCCCACTG 
GACCGGTACG AGCCGAGCCA AGCCAAGACC CGAGCCAGCC TGAGCTGGCT GCTGAGCCGA 
AGCGGACCTG AAGAGAGCGT GTCGTCAGCG GTGTGTGCGG ACGGCTCAGT GTGTGTGTCC 
CTGCAGCGCC GTCTGCTGTC CGCTGAGCTG TACTGTCGTG TGTTCTCGGG TGTGTGTGTG 
TCCGCTGGGG GCGTGGCTCC GGCAGATCTG CCCTCCCTCC TGCTGCTGCT GGACCGCCTC 
CACCTGACGC CGTCGGCCAA TCAGACGCAG CTCAGCCAGA CGCCCATACA CATGGGGGCT 
CATCTGGCTC TCATTGATTC GCTGATGTCA CTGAGCGCCA TGGAAACGGT TGCCCGGGTG 
AAGATGGCGA AGGAGACGGA TCAGTTCGGC TCAGGAAAGA GCTGGGAAAA CTCTCTGCTC 
TTCTGGATCA ACAAGGTCAA TCAGAAGCTG CGGGACAGTG CTGATGTGGA GGAGAATGTG 
AAGCCAAACA CCTGCACAGA CCTGCAGCCG GTGCAGCCCA CGTGTCCTCA GCGCTGGTAC 
TGGAAGCTGG TCCCTCATGC CATCGCATTT TGTTTGAAGG AGTCTGGGAA TAAACCTCCA 
GTGATCCGTT ACAGGAAAGA CAAGGTTCAG TCTAAGCAGA GTCCGGTGTT CTCTGTGGTG 
TCTGGAGTGA AGGATCTCTC CAGCGGCTGC GCCATCGCTG CTGCGCTTCA CTTCTACTGC 
CCTCAGATAC TGCCGCTGGA GGACGTGTGC TTGAAGGACA CCATGTGTGT AGCAGACAGT 
GTGTATAATC TCCAGCTGAT CCGAGAGTTT TATAGAACTC ACCTGAAGAG CTGCTGCCCC 
CTACAGCTGG AGGATTTACT GTACGCCCCG CCCATTCTGC AGGTGAACAT TATGTGCTTC 
CTTTCTGAGC TGTTTGCCAC ATTCGAGGTG CAGAAACCAG ATTTCGTCAA ACCCATACAC 
ACACTGGACC TAACAGATGC ATCAGATCTG GTGGACTGCA CTAGTCCCAT CAACTGCAAC 
AGTGGCTCCC CATCTTTCCT CCTGAAGCAG TCTCTGCCTC AGTCCACCTC AGTGTCGGAA 
GGACGGGGAT GGAGCAAAAA GCAAATAAGC CGTCCTCTCT CGGCGGTGAC CTTCAGCATC 
CCGTTTCCGC TGGACAGTGA TGTTGATGTT GTCATGGGCA ACCCTCTCTT CCGCTCTGTC 
AGCACGGACA GTCTCACTAT GGTAACCAAC CCTGACCCTT ACACTCCTCC AGAGGATCTC 
AGTAAACTCA TTGGTCATGC TCCTCTAGGA GAGCTGCCTA CTATAGAAGA AGCATTACAG 
ATCGTTCACA CTGGCCGTGT GGTACACAAC GAGCCGCGTC TGCGGCCAGA GGGGGCACCA 
TCTGGCTTTT ACCTCCACTC CCAAAATGAG GGTGGAGCCA AACTCAGCAG CTCTGCTCCC 
TGCCGGTCAG GGATTATGTA TCGACCCATT GGAGGAGGGC CTGATAACAG CGGGAACCAC 
AAACGGCAAG ATGGAGGTTC AGAATGTGCC ACGCCTGACT CTCCCAAAAC CTCCAGCCCA 
GCCAACGGAC CCAAGTCTGG CCGCATGACA AACTTTGCTG AGCGCAGAAA AATGATGCCT 
GAATCCCCAA GCAATTCCAG GTCCCAGGAG CGGGAACCTT TGTTCAGCCC TGCAGCAATG 
AGCCCTGTTG GGGCAATGGA GGCGCAGGAG CTGGGTGCAC GTCTGGAGGA GAAGCGGAAA 
GCAATTGAAG CTCAAAAGAG GAGGATTGAG GCCATCTTTA CCAAGCACAG GCAAAGGCTG 
GGCAAAACTG CCTTCCTTCA GCTCCAGAAG AAACAAGAGG GAGAGGTGGA GGAAGATGGA 
CAGTCCATCT CTCTGGATGA ACGACTCACA CAAATGGAGC AGCAGCTTCA ACAAGTGGAG 
GACAGAGAGA AGGAGGAGGA GAAAAGAAAG CAGGAAGAGG GGAAGAGAGA AAAGGATGGG 
GAGGAGAAGA AGAGTGATGT AGTAGCTCCA CCAAAACTAG AGAAACAGGT GACCTTTTCT 
GTTGAGGCAA AGAAAGAGGA GGGGAAAGAG CCACCGTTGG GGGAATATAA CGAGGCAGTA 
GCTAAGCTAA GCTCCACCCT GGAGACTCTG CAAAGAGACA TGCAGCGCCT TACAGAACAG 
CAGCAAAGGC TCATGGGAAA GAAAACTTTG AGTTCTCCAA AAAACATCCC CTCAAGCAAG 
ACCTCAGCAT CCAGCAAGGC CTGGATTATT CCATCTGGTC CCAAATCCCC CGCTACACCA 
CCTCATCTTT CTAGAGACTC CACTCGCATC ATTTCACCCT CTGGCTCTCC ATCACGCTCT 
GGACAACTTT CAGACACACC GAGATCCCCT AAAAGCTCTG CAGCATCCAC CCCTCGTAGA 
TCCCGTAGTT CTGCTCCCAA GAGTCCTAGG CGTCAACGAC CCTCCCGCCC CACGGACCTT 
GGCTTCCAGC CTATCACACG TGTCCTAACA CCGCCGCAGA ATGTGGACAC CCTTCCCCAT 
TTGCGCAGAG TCTCTCCAAG CCAGTGTCAG GTGCAGACCA GCTCATCTCT GCGGCTCGGA 
GGTCCGTACA CCCCACTGGA CTCTTCACCC CAACCTGCCC CTGCTCAAGA GAACACATCA 
GAGTCAGGCT CCAGTGACCA CAACACTCCA ATCTTCAGTC TTGAGCTAGA GGCTGGACCT 
CCGTCTGCAC TCCCAGCGGA GCTGTTAGGA GGAGCAGGCA GCTCTGGAGC TCCATCAGAG 
TGCTCGTTTG AAAGTGACTT GCTTTTGAGC AGTGCCCCCG TGAAAGACGA GACGGAGGGT 
GCCGGAACGA TGGGAGATGA CACCGATTTA GAGCAGGGTG CAGAAATATT CTCATCAGAT 
TCCATGAGTG ACCAGACTGA GAGTGAGAGC CGGCTGGGAC TTGATGTGTT TTTTAAGGAG 
GAGGGTCTCT CGGAGGAGGA GATGGCCCAG AAAAGGGCCC TGCTGTTGGA ACGACAGCAA 
AGACGAGCAC AAGAGCTCAA GAGACGAGCA AGACAAGGGC AGGACCCTGA GAACAGTCAA 
CAGGCTTCCT TGGAAGACCT GCGACCGCAA ACTCCACTGC TATCACAGTC TCTGCCCACC 
TTTTACACCC CACCTCCTCA TTTGAATGCA ACGCCCCCAG GCACGCCCCA GCGCTGGGGA 
GCATTCACAC GGGCGGAGTA TGAACGGCGC CATCAGCTGA AGATAATGGC TGATCTAGGG 
AAGGTTCTCA AACAGAAACC AACAAAGCAA AGTGGCAAAA AGCAGCATGC AAACAAACCA 
CATAACCACG AGCTCATTCA CACACGCTCA CCTGGGAAGA GCAGCACAGC CAACCAGAAA 
GATGGCAGAT CTCTACCAGC TGTAAAACCA GCAAGTCGTT TAGAGTCACC CTCAAAGGTG 
ATGTCATCAG GACTGCTGGC CAGTCAGAAT GGAGAGAAAG ACTGTGAACA AGCCTCCAAC 
GCCTCCTCTC CAGCATCACT CCCAGAATAC ACAGGTCCTA AATTGTTTAA GGAGCCCAGT 
TTTAAATCCA ACAAGTTCAT CATTCACAGT GCGCTCTCAC GCTGCTGCCT TGCTGGCAAA 
GTCAACGAAT CCCAGAAGAA CAAGATCATC GAGGAGATGG AGAAGAGCTC CGCCAGTCAC 
TTTCTGATCC TCATGCGTGA TTCCAACTGT CAGTTCCGTG CCATCTACAC CCTAGACGGC 
CAGTCAGAGG AGCTGCACCG ACTCTGCGGC GTTGGTCCAC GGGTCATTTC CTCATCAGCT 
GTGGAAGCCA TTTATAAATA CAGTTCTGAT AGGAAACAGT TCAATACACT TCCATCCCGC 
ACTCTGAGCA TGAGCGTCGA CGCTTTCACT ATCCCTGGCC AGCTCTGGAA CACTAAGAAA 
CATGGCACGC CCAAAAAAGT AGCCACTCCC AAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003197845.1
CDS	359..4354
Translation	MAMDAPAVKQSAPVPDICPLDRYEPSQAKTRASLSWLLSRSGPEESVSSAVCADGSVCVSLQRRLLSAELYCRVFSGVCVSAGGVAPADLPSLLLLLDRLHLTPSANQTQLSQTPIHMGAHLALIDSLMSLSAMETVARVKMAKETDQFGSGKSWENSLLFWINKVNQKLRDSADVEENVKPNTCTDLQPVQPTCPQRWYWKLVPHAIAFCLKESGNKPPVIRYRKDKVQSKQSPVFSVVSGVKDLSSGCAIAAALHFYCPQILPLEDVCLKDTMCVADSVYNLQLIREFYRTHLKSCCPLQLEDLLYAPPILQVNIMCFLSELFATFEVQKPDFVKPIHTLDLTDASDLVDCTSPINCNSGSPSFLLKQSLPQSTSVSEGRGWSKKQISRPLSAVTFSIPFPLDSDVDVVMGNPLFRSVSTDSLTMVTNPDPYTPPEDLSKLIGHAPLGELPTIEEALQIVHTGRVVHNEPRLRPEGAPSGFYLHSQNEGGAKLSSSAPCRSGIMYRPIGGGPDNSGNHKRQDGGSECATPDSPKTSSPANGPKSGRMTNFAERRKMMPESPSNSRSQEREPLFSPAAMSPVGAMEAQELGARLEEKRKAIEAQKRRIEAIFTKHRQRLGKTAFLQLQKKQEGEVEEDGQSISLDERLTQMEQQLQQVEDREKEEEKRKQEEGKREKDGEEKKSDVVAPPKLEKQVTFSVEAKKEEGKEPPLGEYNEAVAKLSSTLETLQRDMQRLTEQQQRLMGKKTLSSPKNIPSSKTSASSKAWIIPSGPKSPATPPHLSRDSTRIISPSGSPSRSGQLSDTPRSPKSSAASTPRRSRSSAPKSPRRQRPSRPTDLGFQPITRVLTPPQNVDTLPHLRRVSPSQCQVQTSSSLRLGGPYTPLDSSPQPAPAQENTSESGSSDHNTPIFSLELEAGPPSALPAELLGGAGSSGAPSECSFESDLLLSSAPVKDETEGAGTMGDDTDLEQGAEIFSSDSMSDQTESESRLGLDVFFKEEGLSEEEMAQKRALLLERQQRRAQELKRRARQGQDPENSQQASLEDLRPQTPLLSQSLPTFYTPPPHLNATPPGTPQRWGAFTRAEYERRHQLKIMADLGKVLKQKPTKQSGKKQHANKPHNHELIHTRSPGKSSTANQKDGRSLPAVKPASRLESPSKVMSSGLLASQNGEKDCEQASNASSPASLPEYTGPKLFKEPSFKSNKFIIHSALSRCCLAGKVNESQKNKIIEEMEKSSASHFLILMRDSNCQFRAIYTLDGQSEELHRLCGVGPRVISSSAVEAIYKYSSDRKQFNTLPSRTLSMSVDAFTIPGQLWNTKKHGTPKKVATPK

Target ORF information:

RefSeq Version	XM_003197797.4
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calmodulin-regulated spectrin-associated protein 3-like (LOC100007608), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003197797.4

ORF Insert Sequence:

ATGGCGATGG ACGCGCCCGC GGTGAAGCAG AGCGCGCCGG TACCGGACAT CTGCCCACTG 
GACCGGTACG AGCCGAGCCA AGCCAAGACC CGAGCCAGCC TGAGCTGGCT GCTGAGCCGA 
AGCGGACCTG AAGAGAGCGT GTCGTCAGCG GTGTGTGCGG ACGGCTCAGT GTGTGTGTCC 
CTGCAGCGCC GTCTGCTGTC CGCTGAGCTG TACTGTCGTG TGTTCTCGGG TGTGTGTGTG 
TCCGCTGGGG GCGTGGCTCC GGCAGATCTG CCCTCCCTCC TGCTGCTGCT GGACCGCCTC 
CACCTGACGC CGTCGGCCAA TCAGACGCAG CTCAGCCAGA CGCCCATACA CATGGGGGCT 
CATCTGGCTC TCATTGATTC GCTGATGTCA CTGAGCGCCA TGGAAACGGT TGCCCGGGTG 
AAGATGGCGA AGGAGACGGA TCAGTTCGGC TCAGGAAAGA GCTGGGAAAA CTCTCTGCTC 
TTCTGGATCA ACAAGGTCAA TCAGAAGCTG CGGGACAGTG CTGATGTGGA GGAGAATGTG 
AAGCCAAACA CCTGCACAGA CCTGCAGCCG GTGCAGCCCA CGTGTCCTCA GCGCTGGTAC 
TGGAAGCTGG TCCCTCATGC CATCGCATTT TGTTTGAAGG AGTCTGGGAA TAAACCTCCA 
GTGATCCGTT ACAGGAAAGA CAAGGTTCAG TCTAAGCAGA GTCCGGTGTT CTCTGTGGTG 
TCTGGAGTGA AGGATCTCTC CAGCGGCTGC GCCATCGCTG CTGCGCTTCA CTTCTACTGC 
CCTCAGATAC TGCCGCTGGA GGACGTGTGC TTGAAGGACA CCATGTGTGT AGCAGACAGT 
GTGTATAATC TCCAGCTGAT CCGAGAGTTT TATAGAACTC ACCTGAAGAG CTGCTGCCCC 
CTACAGCTGG AGGATTTACT GTACGCCCCG CCCATTCTGC AGGTGAACAT TATGTGCTTC 
CTTTCTGAGC TGTTTGCCAC ATTCGAGGTG CAGAAACCAG ATTTCGTCAA ACCCATACAC 
ACACTGGACC TAACAGATGC ATCAGATCTG GTGGACTGCA CTAGTCCCAT CAACTGCAAC 
AGTGGCTCCC CATCTTTCCT CCTGAAGCAG TCTCTGCCTC AGTCCACCTC AGTGTCGGAA 
GGACGGGGAT GGAGCAAAAA GCAAATAAGC CGTCCTCTCT CGGCGGTGAC CTTCAGCATC 
CCGTTTCCGC TGGACAGTGA TGTTGATGTT GTCATGGGCA ACCCTCTCTT CCGCTCTGTC 
AGCACGGACA GTCTCACTAT GGTAACCAAC CCTGACCCTT ACACTCCTCC AGAGGATCTC 
AGTAAACTCA TTGGTCATGC TCCTCTAGGA GAGCTGCCTA CTATAGAAGA AGCATTACAG 
ATCGTTCACA CTGGCCGTGT GGTACACAAC GAGCCGCGTC TGCGGCCAGA GGGGGCACCA 
TCTGGCTTTT ACCTCCACTC CCAAAATGAG GGTGGAGCCA AACTCAGCAG CTCTGCTCCC 
TGCCGGTCAG GGATTATGTA TCGACCCATT GGAGGAGGGC CTGATAACAG CGGGAACCAC 
AAACGGCAAG ATGGAGGTTC AGAATGTGCC ACGCCTGACT CTCCCAAAAC CTCCAGCCCA 
GCCAACGGAC CCAAGTCTGG CCGCATGACA AACTTTGCTG AGCGCAGAAA AATGATGCCT 
GAATCCCCAA GCAATTCCAG GTCCCAGGAG CGGGAACCTT TGTTCAGCCC TGCAGCAATG 
AGCCCTGTTG GGGCAATGGA GGCGCAGGAG CTGGGTGCAC GTCTGGAGGA GAAGCGGAAA 
GCAATTGAAG CTCAAAAGAG GAGGATTGAG GCCATCTTTA CCAAGCACAG GCAAAGGCTG 
GGCAAAACTG CCTTCCTTCA GCTCCAGAAG AAACAAGAGG GAGAGGTGGA GGAAGATGGA 
CAGTCCATCT CTCTGGATGA ACGACTCACA CAAATGGAGC AGCAGCTTCA ACAAGTGGAG 
GACAGAGAGA AGGAGGAGGA GAAAAGAAAG CAGGAAGAGG GGAAGAGAGA AAAGGATGGG 
GAGGAGAAGA AGAGTGATGT AGTAGCTCCA CCAAAACTAG AGAAACAGGT GACCTTTTCT 
GTTGAGGCAA AGAAAGAGGA GGGGAAAGAG CCACCGTTGG GGGAATATAA CGAGGCAGTA 
GCTAAGCTAA GCTCCACCCT GGAGACTCTG CAAAGAGACA TGCAGCGCCT TACAGAACAG 
CAGCAAAGGC TCATGGGAAA GAAAACTTTG AGTTCTCCAA AAAACATCCC CTCAAGCAAG 
ACCTCAGCAT CCAGCAAGGC CTGGATTATT CCATCTGGTC CCAAATCCCC CGCTACACCA 
CCTCATCTTT CTAGAGACTC CACTCGCATC ATTTCACCCT CTGGCTCTCC ATCACGCTCT 
GGACAACTTT CAGACACACC GAGATCCCCT AAAAGCTCTG CAGCATCCAC CCCTCGTAGA 
TCCCGTAGTT CTGCTCCCAA GAGTCCTAGG CGTCAACGAC CCTCCCGCCC CACGGACCTT 
GGCTTCCAGC CTATCACACG TGTCCTAACA CCGCCGCAGA ATGTGGACAC CCTTCCCCAT 
TTGCGCAGAG TCTCTCCAAG CCAGTGTCAG GTGCAGACCA GCTCATCTCT GCGGCTCGGA 
GGTCCGTACA CCCCACTGGA CTCTTCACCC CAACCTGCCC CTGCTCAAGA GAACACATCA 
GAGTCAGGCT CCAGTGACCA CAACACTCCA ATCTTCAGTC TTGAGCTAGA GGCTGGACCT 
CCGTCTGCAC TCCCAGCGGA GCTGTTAGGA GGAGCAGGCA GCTCTGGAGC TCCATCAGAG 
TGCTCGTTTG AAAGTGACTT GCTTTTGAGC AGTGCCCCCG TGAAAGACGA GACGGAGGGT 
GCCGGAACGA TGGGAGATGA CACCGATTTA GAGCAGGGTG CAGAAATATT CTCATCAGAT 
TCCATGAGTG ACCAGACTGA GAGTGAGAGC CGGCTGGGAC TTGATGTGTT TTTTAAGGAG 
GAGGGTCTCT CGGAGGAGGA GATGGCCCAG AAAAGGGCCC TGCTGTTGGA ACGACAGCAA 
AGACGAGCAC AAGAGCTCAA GAGACGAGCA AGACAAGGGC AGGACCCTGA GAACAGTCAA 
CAGGCTTCCT TGGAAGACCT GCGACCGCAA ACTCCACTGC TATCACAGTC TCTGCCCACC 
TTTTACACCC CACCTCCTCA TTTGAATGCA ACGCCCCCAG GCACGCCCCA GCGCTGGGGA 
GCATTCACAC GGGCGGAGTA TGAACGGCGC CATCAGCTGA AGATAATGGC TGATCTAGGG 
AAGGTTCTCA AACAGAAACC AACAAAGCAA AGTGGCAAAA AGCAGCATGC AAACAAACCA 
CATAACCACG AGCTCATTCA CACACGCTCA CCTGGGAAGA GCAGCACAGC CAACCAGAAA 
GATGGCAGAT CTCTACCAGC TGTAAAACCA GCAAGTCGTT TAGAGTCACC CTCAAAGGTG 
ATGTCATCAG GACTGCTGGC CAGTCAGAAT GGAGAGAAAG ACTGTGAACA AGCCTCCAAC 
GCCTCCTCTC CAGCATCACT CCCAGAATAC ACAGGTCCTA AATTGTTTAA GGAGCCCAGT 
TTTAAATCCA ACAAGTTCAT CATTCACAGT GCGCTCTCAC GCTGCTGCCT TGCTGGCAAA 
GTCAACGAAT CCCAGAAGAA CAAGATCATC GAGGAGATGG AGAAGAGCTC CGCCAGTCAC 
TTTCTGATCC TCATGCGTGA TTCCAACTGT CAGTTCCGTG CCATCTACAC CCTAGACGGC 
CAGTCAGAGG AGCTGCACCG ACTCTGCGGC GTTGGTCCAC GGGTCATTTC CTCATCAGCT 
GTGGAAGCCA TTTATAAATA CAGTTCTGAT AGGAAACAGT TCAATACACT TCCATCCCGC 
ACTCTGAGCA TGAGCGTCGA CGCTTTCACT ATCCCTGGCC AGCTCTGGAA CACTAAGAAA 
CATGGCACGC CCAAAAAAGT AGCCACTCCC AAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100007608 ( XM_003197797.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_003197845.1 Danio rerio calmodulin-regulated spectrin-associated protein 3-like (LOC100007608), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa06526
Clone ID Related Accession (Same CDS sequence)	XM_003197797.4 , XM_003197797.5
Accession Version	XM_003197797.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	calmodulin-regulated spectrin-associated protein 3-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_003197797.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGATGG ACGCGCCCGC GGTGAAGCAG AGCGCGCCGG TACCGGACAT CTGCCCACTG 
GACCGGTACG AGCCGAGCCA AGCCAAGACC CGAGCCAGCC TGAGCTGGCT GCTGAGCCGA 
AGCGGACCTG AAGAGAGCGT GTCGTCAGCG GTGTGTGCGG ACGGCTCAGT GTGTGTGTCC 
CTGCAGCGCC GTCTGCTGTC CGCTGAGCTG TACTGTCGTG TGTTCTCGGG TGTGTGTGTG 
TCCGCTGGGG GCGTGGCTCC GGCAGATCTG CCCTCCCTCC TGCTGCTGCT GGACCGCCTC 
CACCTGACGC CGTCGGCCAA TCAGACGCAG CTCAGCCAGA CGCCCATACA CATGGGGGCT 
CATCTGGCTC TCATTGATTC GCTGATGTCA CTGAGCGCCA TGGAAACGGT TGCCCGGGTG 
AAGATGGCGA AGGAGACGGA TCAGTTCGGC TCAGGAAAGA GCTGGGAAAA CTCTCTGCTC 
TTCTGGATCA ACAAGGTCAA TCAGAAGCTG CGGGACAGTG CTGATGTGGA GGAGAATGTG 
AAGCCAAACA CCTGCACAGA CCTGCAGCCG GTGCAGCCCA CGTGTCCTCA GCGCTGGTAC 
TGGAAGCTGG TCCCTCATGC CATCGCATTT TGTTTGAAGG AGTCTGGGAA TAAACCTCCA 
GTGATCCGTT ACAGGAAAGA CAAGGTTCAG TCTAAGCAGA GTCCGGTGTT CTCTGTGGTG 
TCTGGAGTGA AGGATCTCTC CAGCGGCTGC GCCATCGCTG CTGCGCTTCA CTTCTACTGC 
CCTCAGATAC TGCCGCTGGA GGACGTGTGC TTGAAGGACA CCATGTGTGT AGCAGACAGT 
GTGTATAATC TCCAGCTGAT CCGAGAGTTT TATAGAACTC ACCTGAAGAG CTGCTGCCCC 
CTACAGCTGG AGGATTTACT GTACGCCCCG CCCATTCTGC AGGTGAACAT TATGTGCTTC 
CTTTCTGAGC TGTTTGCCAC ATTCGAGGTG CAGAAACCAG ATTTCGTCAA ACCCATACAC 
ACACTGGACC TAACAGATGC ATCAGATCTG GTGGACTGCA CTAGTCCCAT CAACTGCAAC 
AGTGGCTCCC CATCTTTCCT CCTGAAGCAG TCTCTGCCTC AGTCCACCTC AGTGTCGGAA 
GGACGGGGAT GGAGCAAAAA GCAAATAAGC CGTCCTCTCT CGGCGGTGAC CTTCAGCATC 
CCGTTTCCGC TGGACAGTGA TGTTGATGTT GTCATGGGCA ACCCTCTCTT CCGCTCTGTC 
AGCACGGACA GTCTCACTAT GGTAACCAAC CCTGACCCTT ACACTCCTCC AGAGGATCTC 
AGTAAACTCA TTGGTCATGC TCCTCTAGGA GAGCTGCCTA CTATAGAAGA AGCATTACAG 
ATCGTTCACA CTGGCCGTGT GGTACACAAC GAGCCGCGTC TGCGGCCAGA GGGGGCACCA 
TCTGGCTTTT ACCTCCACTC CCAAAATGAG GGTGGAGCCA AACTCAGCAG CTCTGCTCCC 
TGCCGGTCAG GGATTATGTA TCGACCCATT GGAGGAGGGC CTGATAACAG CGGGAACCAC 
AAACGGCAAG ATGGAGGTTC AGAATGTGCC ACGCCTGACT CTCCCAAAAC CTCCAGCCCA 
GCCAACGGAC CCAAGTCTGG CCGCATGACA AACTTTGCTG AGCGCAGAAA AATGATGCCT 
GAATCCCCAA GCAATTCCAG GTCCCAGGAG CGGGAACCTT TGTTCAGCCC TGCAGCAATG 
AGCCCTGTTG GGGCAATGGA GGCGCAGGAG CTGGGTGCAC GTCTGGAGGA GAAGCGGAAA 
GCAATTGAAG CTCAAAAGAG GAGGATTGAG GCCATCTTTA CCAAGCACAG GCAAAGGCTG 
GGCAAAACTG CCTTCCTTCA GCTCCAGAAG AAACAAGAGG GAGAGGTGGA GGAAGATGGA 
CAGTCCATCT CTCTGGATGA ACGACTCACA CAAATGGAGC AGCAGCTTCA ACAAGTGGAG 
GACAGAGAGA AGGAGGAGGA GAAAAGAAAG CAGGAAGAGG GGAAGAGAGA AAAGGATGGG 
GAGGAGAAGA AGAGTGATGT AGTAGCTCCA CCAAAACTAG AGAAACAGGT GACCTTTTCT 
GTTGAGGCAA AGAAAGAGGA GGGGAAAGAG CCACCGTTGG GGGAATATAA CGAGGCAGTA 
GCTAAGCTAA GCTCCACCCT GGAGACTCTG CAAAGAGACA TGCAGCGCCT TACAGAACAG 
CAGCAAAGGC TCATGGGAAA GAAAACTTTG AGTTCTCCAA AAAACATCCC CTCAAGCAAG 
ACCTCAGCAT CCAGCAAGGC CTGGATTATT CCATCTGGTC CCAAATCCCC CGCTACACCA 
CCTCATCTTT CTAGAGACTC CACTCGCATC ATTTCACCCT CTGGCTCTCC ATCACGCTCT 
GGACAACTTT CAGACACACC GAGATCCCCT AAAAGCTCTG CAGCATCCAC CCCTCGTAGA 
TCCCGTAGTT CTGCTCCCAA GAGTCCTAGG CGTCAACGAC CCTCCCGCCC CACGGACCTT 
GGCTTCCAGC CTATCACACG TGTCCTAACA CCGCCGCAGA ATGTGGACAC CCTTCCCCAT 
TTGCGCAGAG TCTCTCCAAG CCAGTGTCAG GTGCAGACCA GCTCATCTCT GCGGCTCGGA 
GGTCCGTACA CCCCACTGGA CTCTTCACCC CAACCTGCCC CTGCTCAAGA GAACACATCA 
GAGTCAGGCT CCAGTGACCA CAACACTCCA ATCTTCAGTC TTGAGCTAGA GGCTGGACCT 
CCGTCTGCAC TCCCAGCGGA GCTGTTAGGA GGAGCAGGCA GCTCTGGAGC TCCATCAGAG 
TGCTCGTTTG AAAGTGACTT GCTTTTGAGC AGTGCCCCCG TGAAAGACGA GACGGAGGGT 
GCCGGAACGA TGGGAGATGA CACCGATTTA GAGCAGGGTG CAGAAATATT CTCATCAGAT 
TCCATGAGTG ACCAGACTGA GAGTGAGAGC CGGCTGGGAC TTGATGTGTT TTTTAAGGAG 
GAGGGTCTCT CGGAGGAGGA GATGGCCCAG AAAAGGGCCC TGCTGTTGGA ACGACAGCAA 
AGACGAGCAC AAGAGCTCAA GAGACGAGCA AGACAAGGGC AGGACCCTGA GAACAGTCAA 
CAGGCTTCCT TGGAAGACCT GCGACCGCAA ACTCCACTGC TATCACAGTC TCTGCCCACC 
TTTTACACCC CACCTCCTCA TTTGAATGCA ACGCCCCCAG GCACGCCCCA GCGCTGGGGA 
GCATTCACAC GGGCGGAGTA TGAACGGCGC CATCAGCTGA AGATAATGGC TGATCTAGGG 
AAGGTTCTCA AACAGAAACC AACAAAGCAA AGTGGCAAAA AGCAGCATGC AAACAAACCA 
CATAACCACG AGCTCATTCA CACACGCTCA CCTGGGAAGA GCAGCACAGC CAACCAGAAA 
GATGGCAGAT CTCTACCAGC TGTAAAACCA GCAAGTCGTT TAGAGTCACC CTCAAAGGTG 
ATGTCATCAG GACTGCTGGC CAGTCAGAAT GGAGAGAAAG ACTGTGAACA AGCCTCCAAC 
GCCTCCTCTC CAGCATCACT CCCAGAATAC ACAGGTCCTA AATTGTTTAA GGAGCCCAGT 
TTTAAATCCA ACAAGTTCAT CATTCACAGT GCGCTCTCAC GCTGCTGCCT TGCTGGCAAA 
GTCAACGAAT CCCAGAAGAA CAAGATCATC GAGGAGATGG AGAAGAGCTC CGCCAGTCAC 
TTTCTGATCC TCATGCGTGA TTCCAACTGT CAGTTCCGTG CCATCTACAC CCTAGACGGC 
CAGTCAGAGG AGCTGCACCG ACTCTGCGGC GTTGGTCCAC GGGTCATTTC CTCATCAGCT 
GTGGAAGCCA TTTATAAATA CAGTTCTGAT AGGAAACAGT TCAATACACT TCCATCCCGC 
ACTCTGAGCA TGAGCGTCGA CGCTTTCACT ATCCCTGGCC AGCTCTGGAA CACTAAGAAA 
CATGGCACGC CCAAAAAAGT AGCCACTCCC AAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003197845.1
CDS	184..4179
Translation	MAMDAPAVKQSAPVPDICPLDRYEPSQAKTRASLSWLLSRSGPEESVSSAVCADGSVCVSLQRRLLSAELYCRVFSGVCVSAGGVAPADLPSLLLLLDRLHLTPSANQTQLSQTPIHMGAHLALIDSLMSLSAMETVARVKMAKETDQFGSGKSWENSLLFWINKVNQKLRDSADVEENVKPNTCTDLQPVQPTCPQRWYWKLVPHAIAFCLKESGNKPPVIRYRKDKVQSKQSPVFSVVSGVKDLSSGCAIAAALHFYCPQILPLEDVCLKDTMCVADSVYNLQLIREFYRTHLKSCCPLQLEDLLYAPPILQVNIMCFLSELFATFEVQKPDFVKPIHTLDLTDASDLVDCTSPINCNSGSPSFLLKQSLPQSTSVSEGRGWSKKQISRPLSAVTFSIPFPLDSDVDVVMGNPLFRSVSTDSLTMVTNPDPYTPPEDLSKLIGHAPLGELPTIEEALQIVHTGRVVHNEPRLRPEGAPSGFYLHSQNEGGAKLSSSAPCRSGIMYRPIGGGPDNSGNHKRQDGGSECATPDSPKTSSPANGPKSGRMTNFAERRKMMPESPSNSRSQEREPLFSPAAMSPVGAMEAQELGARLEEKRKAIEAQKRRIEAIFTKHRQRLGKTAFLQLQKKQEGEVEEDGQSISLDERLTQMEQQLQQVEDREKEEEKRKQEEGKREKDGEEKKSDVVAPPKLEKQVTFSVEAKKEEGKEPPLGEYNEAVAKLSSTLETLQRDMQRLTEQQQRLMGKKTLSSPKNIPSSKTSASSKAWIIPSGPKSPATPPHLSRDSTRIISPSGSPSRSGQLSDTPRSPKSSAASTPRRSRSSAPKSPRRQRPSRPTDLGFQPITRVLTPPQNVDTLPHLRRVSPSQCQVQTSSSLRLGGPYTPLDSSPQPAPAQENTSESGSSDHNTPIFSLELEAGPPSALPAELLGGAGSSGAPSECSFESDLLLSSAPVKDETEGAGTMGDDTDLEQGAEIFSSDSMSDQTESESRLGLDVFFKEEGLSEEEMAQKRALLLERQQRRAQELKRRARQGQDPENSQQASLEDLRPQTPLLSQSLPTFYTPPPHLNATPPGTPQRWGAFTRAEYERRHQLKIMADLGKVLKQKPTKQSGKKQHANKPHNHELIHTRSPGKSSTANQKDGRSLPAVKPASRLESPSKVMSSGLLASQNGEKDCEQASNASSPASLPEYTGPKLFKEPSFKSNKFIIHSALSRCCLAGKVNESQKNKIIEEMEKSSASHFLILMRDSNCQFRAIYTLDGQSEELHRLCGVGPRVISSSAVEAIYKYSSDRKQFNTLPSRTLSMSVDAFTIPGQLWNTKKHGTPKKVATPK

Target ORF information:

RefSeq Version	XM_003197797.5
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calmodulin-regulated spectrin-associated protein 3-like (LOC100007608), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003197797.5

ORF Insert Sequence:

ATGGCGATGG ACGCGCCCGC GGTGAAGCAG AGCGCGCCGG TACCGGACAT CTGCCCACTG 
GACCGGTACG AGCCGAGCCA AGCCAAGACC CGAGCCAGCC TGAGCTGGCT GCTGAGCCGA 
AGCGGACCTG AAGAGAGCGT GTCGTCAGCG GTGTGTGCGG ACGGCTCAGT GTGTGTGTCC 
CTGCAGCGCC GTCTGCTGTC CGCTGAGCTG TACTGTCGTG TGTTCTCGGG TGTGTGTGTG 
TCCGCTGGGG GCGTGGCTCC GGCAGATCTG CCCTCCCTCC TGCTGCTGCT GGACCGCCTC 
CACCTGACGC CGTCGGCCAA TCAGACGCAG CTCAGCCAGA CGCCCATACA CATGGGGGCT 
CATCTGGCTC TCATTGATTC GCTGATGTCA CTGAGCGCCA TGGAAACGGT TGCCCGGGTG 
AAGATGGCGA AGGAGACGGA TCAGTTCGGC TCAGGAAAGA GCTGGGAAAA CTCTCTGCTC 
TTCTGGATCA ACAAGGTCAA TCAGAAGCTG CGGGACAGTG CTGATGTGGA GGAGAATGTG 
AAGCCAAACA CCTGCACAGA CCTGCAGCCG GTGCAGCCCA CGTGTCCTCA GCGCTGGTAC 
TGGAAGCTGG TCCCTCATGC CATCGCATTT TGTTTGAAGG AGTCTGGGAA TAAACCTCCA 
GTGATCCGTT ACAGGAAAGA CAAGGTTCAG TCTAAGCAGA GTCCGGTGTT CTCTGTGGTG 
TCTGGAGTGA AGGATCTCTC CAGCGGCTGC GCCATCGCTG CTGCGCTTCA CTTCTACTGC 
CCTCAGATAC TGCCGCTGGA GGACGTGTGC TTGAAGGACA CCATGTGTGT AGCAGACAGT 
GTGTATAATC TCCAGCTGAT CCGAGAGTTT TATAGAACTC ACCTGAAGAG CTGCTGCCCC 
CTACAGCTGG AGGATTTACT GTACGCCCCG CCCATTCTGC AGGTGAACAT TATGTGCTTC 
CTTTCTGAGC TGTTTGCCAC ATTCGAGGTG CAGAAACCAG ATTTCGTCAA ACCCATACAC 
ACACTGGACC TAACAGATGC ATCAGATCTG GTGGACTGCA CTAGTCCCAT CAACTGCAAC 
AGTGGCTCCC CATCTTTCCT CCTGAAGCAG TCTCTGCCTC AGTCCACCTC AGTGTCGGAA 
GGACGGGGAT GGAGCAAAAA GCAAATAAGC CGTCCTCTCT CGGCGGTGAC CTTCAGCATC 
CCGTTTCCGC TGGACAGTGA TGTTGATGTT GTCATGGGCA ACCCTCTCTT CCGCTCTGTC 
AGCACGGACA GTCTCACTAT GGTAACCAAC CCTGACCCTT ACACTCCTCC AGAGGATCTC 
AGTAAACTCA TTGGTCATGC TCCTCTAGGA GAGCTGCCTA CTATAGAAGA AGCATTACAG 
ATCGTTCACA CTGGCCGTGT GGTACACAAC GAGCCGCGTC TGCGGCCAGA GGGGGCACCA 
TCTGGCTTTT ACCTCCACTC CCAAAATGAG GGTGGAGCCA AACTCAGCAG CTCTGCTCCC 
TGCCGGTCAG GGATTATGTA TCGACCCATT GGAGGAGGGC CTGATAACAG CGGGAACCAC 
AAACGGCAAG ATGGAGGTTC AGAATGTGCC ACGCCTGACT CTCCCAAAAC CTCCAGCCCA 
GCCAACGGAC CCAAGTCTGG CCGCATGACA AACTTTGCTG AGCGCAGAAA AATGATGCCT 
GAATCCCCAA GCAATTCCAG GTCCCAGGAG CGGGAACCTT TGTTCAGCCC TGCAGCAATG 
AGCCCTGTTG GGGCAATGGA GGCGCAGGAG CTGGGTGCAC GTCTGGAGGA GAAGCGGAAA 
GCAATTGAAG CTCAAAAGAG GAGGATTGAG GCCATCTTTA CCAAGCACAG GCAAAGGCTG 
GGCAAAACTG CCTTCCTTCA GCTCCAGAAG AAACAAGAGG GAGAGGTGGA GGAAGATGGA 
CAGTCCATCT CTCTGGATGA ACGACTCACA CAAATGGAGC AGCAGCTTCA ACAAGTGGAG 
GACAGAGAGA AGGAGGAGGA GAAAAGAAAG CAGGAAGAGG GGAAGAGAGA AAAGGATGGG 
GAGGAGAAGA AGAGTGATGT AGTAGCTCCA CCAAAACTAG AGAAACAGGT GACCTTTTCT 
GTTGAGGCAA AGAAAGAGGA GGGGAAAGAG CCACCGTTGG GGGAATATAA CGAGGCAGTA 
GCTAAGCTAA GCTCCACCCT GGAGACTCTG CAAAGAGACA TGCAGCGCCT TACAGAACAG 
CAGCAAAGGC TCATGGGAAA GAAAACTTTG AGTTCTCCAA AAAACATCCC CTCAAGCAAG 
ACCTCAGCAT CCAGCAAGGC CTGGATTATT CCATCTGGTC CCAAATCCCC CGCTACACCA 
CCTCATCTTT CTAGAGACTC CACTCGCATC ATTTCACCCT CTGGCTCTCC ATCACGCTCT 
GGACAACTTT CAGACACACC GAGATCCCCT AAAAGCTCTG CAGCATCCAC CCCTCGTAGA 
TCCCGTAGTT CTGCTCCCAA GAGTCCTAGG CGTCAACGAC CCTCCCGCCC CACGGACCTT 
GGCTTCCAGC CTATCACACG TGTCCTAACA CCGCCGCAGA ATGTGGACAC CCTTCCCCAT 
TTGCGCAGAG TCTCTCCAAG CCAGTGTCAG GTGCAGACCA GCTCATCTCT GCGGCTCGGA 
GGTCCGTACA CCCCACTGGA CTCTTCACCC CAACCTGCCC CTGCTCAAGA GAACACATCA 
GAGTCAGGCT CCAGTGACCA CAACACTCCA ATCTTCAGTC TTGAGCTAGA GGCTGGACCT 
CCGTCTGCAC TCCCAGCGGA GCTGTTAGGA GGAGCAGGCA GCTCTGGAGC TCCATCAGAG 
TGCTCGTTTG AAAGTGACTT GCTTTTGAGC AGTGCCCCCG TGAAAGACGA GACGGAGGGT 
GCCGGAACGA TGGGAGATGA CACCGATTTA GAGCAGGGTG CAGAAATATT CTCATCAGAT 
TCCATGAGTG ACCAGACTGA GAGTGAGAGC CGGCTGGGAC TTGATGTGTT TTTTAAGGAG 
GAGGGTCTCT CGGAGGAGGA GATGGCCCAG AAAAGGGCCC TGCTGTTGGA ACGACAGCAA 
AGACGAGCAC AAGAGCTCAA GAGACGAGCA AGACAAGGGC AGGACCCTGA GAACAGTCAA 
CAGGCTTCCT TGGAAGACCT GCGACCGCAA ACTCCACTGC TATCACAGTC TCTGCCCACC 
TTTTACACCC CACCTCCTCA TTTGAATGCA ACGCCCCCAG GCACGCCCCA GCGCTGGGGA 
GCATTCACAC GGGCGGAGTA TGAACGGCGC CATCAGCTGA AGATAATGGC TGATCTAGGG 
AAGGTTCTCA AACAGAAACC AACAAAGCAA AGTGGCAAAA AGCAGCATGC AAACAAACCA 
CATAACCACG AGCTCATTCA CACACGCTCA CCTGGGAAGA GCAGCACAGC CAACCAGAAA 
GATGGCAGAT CTCTACCAGC TGTAAAACCA GCAAGTCGTT TAGAGTCACC CTCAAAGGTG 
ATGTCATCAG GACTGCTGGC CAGTCAGAAT GGAGAGAAAG ACTGTGAACA AGCCTCCAAC 
GCCTCCTCTC CAGCATCACT CCCAGAATAC ACAGGTCCTA AATTGTTTAA GGAGCCCAGT 
TTTAAATCCA ACAAGTTCAT CATTCACAGT GCGCTCTCAC GCTGCTGCCT TGCTGGCAAA 
GTCAACGAAT CCCAGAAGAA CAAGATCATC GAGGAGATGG AGAAGAGCTC CGCCAGTCAC 
TTTCTGATCC TCATGCGTGA TTCCAACTGT CAGTTCCGTG CCATCTACAC CCTAGACGGC 
CAGTCAGAGG AGCTGCACCG ACTCTGCGGC GTTGGTCCAC GGGTCATTTC CTCATCAGCT 
GTGGAAGCCA TTTATAAATA CAGTTCTGAT AGGAAACAGT TCAATACACT TCCATCCCGC 
ACTCTGAGCA TGAGCGTCGA CGCTTTCACT ATCCCTGGCC AGCTCTGGAA CACTAAGAAA 
CATGGCACGC CCAAAAAAGT AGCCACTCCC AAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100007608 ( XM_005171060.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171117.1 Danio rerio calmodulin-regulated spectrin-associated protein 3-like (LOC100007608), transcript variant X2, mRNA.

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CloneID	ODa06527
Clone ID Related Accession (Same CDS sequence)	XM_005171060.3 , XM_005171060.4
Accession Version	XM_005171060.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	calmodulin-regulated spectrin-associated protein 3-like isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005171060.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGATGG ACGCGCCCGC GGTGAAGCAG AGCGCGCCGG TACCGGACAT CTGCCCACTG 
GACCGGTACG AGCCGAGCCA AGCCAAGACC CGAGCCAGCC TGAGCTGGCT GCTGAGCCGA 
AGCGGACCTG AAGAGAGCGT GTCGTCAGCG GTGTGTGCGG ACGGCTCAGT GTGTGTGTCC 
CTGCAGCGCC GTCTGCTGTC CGCTGAGCTG TACTGTCGTG TGTTCTCGGG TGTGTGTGTG 
TCCGCTGGGG GCGTGGCTCC GGCAGATCTG CCCTCCCTCC TGCTGCTGCT GGACCGCCTC 
CACCTGACGC CGTCGGCCAA TCAGACGCAG CTCAGCCAGA CGCCCATACA CATGGGGGCT 
CATCTGGCTC TCATTGATTC GCTGATGTCA CTGAGCGCCA TGGAAACGGT TGCCCGGGTG 
AAGATGGCGA AGGAGACGGA TCAGTTCGGC TCAGGAAAGA GCTGGGAAAA CTCTCTGCTC 
TTCTGGATCA ACAAGGTCAA TCAGAAGCTG CGGGACAGTG CTGATGTGGA GGAGAATGTG 
AAGCCAAACA CCTGCACAGA CCTGCAGCCG GTGCAGCCCA CGTGTCCTCA GCGCTGGTAC 
TGGAAGCTGG TCCCTCATGC CATCGCATTT TGTTTGAAGG AGTCTGGGAA TAAACCTCCA 
GTGATCCGTT ACAGGAAAGA CAAGGTTCAG TCTAAGCAGA GTCCGGTGTT CTCTGTGGTG 
TCTGGAGTGA AGGATCTCTC CAGCGGCTGC GCCATCGCTG CTGCGCTTCA CTTCTACTGC 
CCTCAGATAC TGCCGCTGGA GGACGTGTGC TTGAAGGACA CCATGTGTGT AGCAGACAGT 
GTGTATAATC TCCAGCTGAT CCGAGAGTTT TATAGAACTC ACCTGAAGAG CTGCTGCCCC 
CTACAGCTGG AGGATTTACT GTACGCCCCG CCCATTCTGC AGGTGAACAT TATGTGCTTC 
CTTTCTGAGC TGTTTGCCAC ATTCGAGGTG CAGAAACCAG ATTTCGTCAA ACCCATACAC 
ACACTGGACC TAACAGATGC ATCAGATCTG GTGGACTGCA CTAGTCCCAT CAACTGCAAC 
AGTGGCTCCC CATCTTTCCT CCTGAAGCAG TCTCTGCCTC AGTCCACCTC AGTGTCGGGA 
CGGGGATGGA GCAAAAAGCA AATAAGCCGT CCTCTCTCGG CGGTGACCTT CAGCATCCCG 
TTTCCGCTGG ACAGTGATGT TGATGTTGTC ATGGGCAACC CTCTCTTCCG CTCTGTCAGC 
ACGGACAGTC TCACTATGGT AACCAACCCT GACCCTTACA CTCCTCCAGA GGATCTCAGT 
AAACTCATTG GTCATGCTCC TCTAGGAGAG CTGCCTACTA TAGAAGAAGC ATTACAGATC 
GTTCACACTG GCCGTGTGGT ACACAACGAG CCGCGTCTGC GGCCAGAGGG GGCACCATCT 
GGCTTTTACC TCCACTCCCA AAATGAGGGT GGAGCCAAAC TCAGCAGCTC TGCTCCCTGC 
CGGTCAGGGA TTATGTATCG ACCCATTGGA GGAGGGCCTG ATAACAGCGG GAACCACAAA 
CGGCAAGATG GAGGTTCAGA ATGTGCCACG CCTGACTCTC CCAAAACCTC CAGCCCAGCC 
AACGGACCCA AGTCTGGCCG CATGACAAAC TTTGCTGAGC GCAGAAAAAT GATGCCTGAA 
TCCCCAAGCA ATTCCAGGTC CCAGGAGCGG GAACCTTTGT TCAGCCCTGC AGCAATGAGC 
CCTGTTGGGG CAATGGAGGC GCAGGAGCTG GGTGCACGTC TGGAGGAGAA GCGGAAAGCA 
ATTGAAGCTC AAAAGAGGAG GATTGAGGCC ATCTTTACCA AGCACAGGCA AAGGCTGGGC 
AAAACTGCCT TCCTTCAGCT CCAGAAGAAA CAAGAGGGAG AGGTGGAGGA AGATGGACAG 
TCCATCTCTC TGGATGAACG ACTCACACAA ATGGAGCAGC AGCTTCAACA AGTGGAGGAC 
AGAGAGAAGG AGGAGGAGAA AAGAAAGCAG GAAGAGGGGA AGAGAGAAAA GGATGGGGAG 
GAGAAGAAGA GTGATGTAGT AGCTCCACCA AAACTAGAGA AACAGGTGAC CTTTTCTGTT 
GAGGCAAAGA AAGAGGAGGG GAAAGAGCCA CCGTTGGGGG AATATAACGA GGCAGTAGCT 
AAGCTAAGCT CCACCCTGGA GACTCTGCAA AGAGACATGC AGCGCCTTAC AGAACAGCAG 
CAAAGGCTCA TGGGAAAGAA AACTTTGAGT TCTCCAAAAA ACATCCCCTC AAGCAAGACC 
TCAGCATCCA GCAAGGCCTG GATTATTCCA TCTGGTCCCA AATCCCCCGC TACACCACCT 
CATCTTTCTA GAGACTCCAC TCGCATCATT TCACCCTCTG GCTCTCCATC ACGCTCTGGA 
CAACTTTCAG ACACACCGAG ATCCCCTAAA AGCTCTGCAG CATCCACCCC TCGTAGATCC 
CGTAGTTCTG CTCCCAAGAG TCCTAGGCGT CAACGACCCT CCCGCCCCAC GGACCTTGGC 
TTCCAGCCTA TCACACGTGT CCTAACACCG CCGCAGAATG TGGACACCCT TCCCCATTTG 
CGCAGAGTCT CTCCAAGCCA GTGTCAGGTG CAGACCAGCT CATCTCTGCG GCTCGGAGGT 
CCGTACACCC CACTGGACTC TTCACCCCAA CCTGCCCCTG CTCAAGAGAA CACATCAGAG 
TCAGGCTCCA GTGACCACAA CACTCCAATC TTCAGTCTTG AGCTAGAGGC TGGACCTCCG 
TCTGCACTCC CAGCGGAGCT GTTAGGAGGA GCAGGCAGCT CTGGAGCTCC ATCAGAGTGC 
TCGTTTGAAA GTGACTTGCT TTTGAGCAGT GCCCCCGTGA AAGACGAGAC GGAGGGTGCC 
GGAACGATGG GAGATGACAC CGATTTAGAG CAGGGTGCAG AAATATTCTC ATCAGATTCC 
ATGAGTGACC AGACTGAGAG TGAGAGCCGG CTGGGACTTG ATGTGTTTTT TAAGGAGGAG 
GGTCTCTCGG AGGAGGAGAT GGCCCAGAAA AGGGCCCTGC TGTTGGAACG ACAGCAAAGA 
CGAGCACAAG AGCTCAAGAG ACGAGCAAGA CAAGGGCAGG ACCCTGAGAA CAGTCAACAG 
GCTTCCTTGG AAGACCTGCG ACCGCAAACT CCACTGCTAT CACAGTCTCT GCCCACCTTT 
TACACCCCAC CTCCTCATTT GAATGCAACG CCCCCAGGCA CGCCCCAGCG CTGGGGAGCA 
TTCACACGGG CGGAGTATGA ACGGCGCCAT CAGCTGAAGA TAATGGCTGA TCTAGGGAAG 
GTTCTCAAAC AGAAACCAAC AAAGCAAAGT GGCAAAAAGC AGCATGCAAA CAAACCACAT 
AACCACGAGC TCATTCACAC ACGCTCACCT GGGAAGAGCA GCACAGCCAA CCAGAAAGAT 
GGCAGATCTC TACCAGCTGT AAAACCAGCA AGTCGTTTAG AGTCACCCTC AAAGGTGATG 
TCATCAGGAC TGCTGGCCAG TCAGAATGGA GAGAAAGACT GTGAACAAGC CTCCAACGCC 
TCCTCTCCAG CATCACTCCC AGAATACACA GGTCCTAAAT TGTTTAAGGA GCCCAGTTTT 
AAATCCAACA AGTTCATCAT TCACAGTGCG CTCTCACGCT GCTGCCTTGC TGGCAAAGTC 
AACGAATCCC AGAAGAACAA GATCATCGAG GAGATGGAGA AGAGCTCCGC CAGTCACTTT 
CTGATCCTCA TGCGTGATTC CAACTGTCAG TTCCGTGCCA TCTACACCCT AGACGGCCAG 
TCAGAGGAGC TGCACCGACT CTGCGGCGTT GGTCCACGGG TCATTTCCTC ATCAGCTGTG 
GAAGCCATTT ATAAATACAG TTCTGATAGG AAACAGTTCA ATACACTTCC ATCCCGCACT 
CTGAGCATGA GCGTCGACGC TTTCACTATC CCTGGCCAGC TCTGGAACAC TAAGAAACAT 
GGCACGCCCA AAAAAGTAGC CACTCCCAAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171117.1
CDS	359..4351
Translation	MAMDAPAVKQSAPVPDICPLDRYEPSQAKTRASLSWLLSRSGPEESVSSAVCADGSVCVSLQRRLLSAELYCRVFSGVCVSAGGVAPADLPSLLLLLDRLHLTPSANQTQLSQTPIHMGAHLALIDSLMSLSAMETVARVKMAKETDQFGSGKSWENSLLFWINKVNQKLRDSADVEENVKPNTCTDLQPVQPTCPQRWYWKLVPHAIAFCLKESGNKPPVIRYRKDKVQSKQSPVFSVVSGVKDLSSGCAIAAALHFYCPQILPLEDVCLKDTMCVADSVYNLQLIREFYRTHLKSCCPLQLEDLLYAPPILQVNIMCFLSELFATFEVQKPDFVKPIHTLDLTDASDLVDCTSPINCNSGSPSFLLKQSLPQSTSVSGRGWSKKQISRPLSAVTFSIPFPLDSDVDVVMGNPLFRSVSTDSLTMVTNPDPYTPPEDLSKLIGHAPLGELPTIEEALQIVHTGRVVHNEPRLRPEGAPSGFYLHSQNEGGAKLSSSAPCRSGIMYRPIGGGPDNSGNHKRQDGGSECATPDSPKTSSPANGPKSGRMTNFAERRKMMPESPSNSRSQEREPLFSPAAMSPVGAMEAQELGARLEEKRKAIEAQKRRIEAIFTKHRQRLGKTAFLQLQKKQEGEVEEDGQSISLDERLTQMEQQLQQVEDREKEEEKRKQEEGKREKDGEEKKSDVVAPPKLEKQVTFSVEAKKEEGKEPPLGEYNEAVAKLSSTLETLQRDMQRLTEQQQRLMGKKTLSSPKNIPSSKTSASSKAWIIPSGPKSPATPPHLSRDSTRIISPSGSPSRSGQLSDTPRSPKSSAASTPRRSRSSAPKSPRRQRPSRPTDLGFQPITRVLTPPQNVDTLPHLRRVSPSQCQVQTSSSLRLGGPYTPLDSSPQPAPAQENTSESGSSDHNTPIFSLELEAGPPSALPAELLGGAGSSGAPSECSFESDLLLSSAPVKDETEGAGTMGDDTDLEQGAEIFSSDSMSDQTESESRLGLDVFFKEEGLSEEEMAQKRALLLERQQRRAQELKRRARQGQDPENSQQASLEDLRPQTPLLSQSLPTFYTPPPHLNATPPGTPQRWGAFTRAEYERRHQLKIMADLGKVLKQKPTKQSGKKQHANKPHNHELIHTRSPGKSSTANQKDGRSLPAVKPASRLESPSKVMSSGLLASQNGEKDCEQASNASSPASLPEYTGPKLFKEPSFKSNKFIIHSALSRCCLAGKVNESQKNKIIEEMEKSSASHFLILMRDSNCQFRAIYTLDGQSEELHRLCGVGPRVISSSAVEAIYKYSSDRKQFNTLPSRTLSMSVDAFTIPGQLWNTKKHGTPKKVATPK

Target ORF information:

RefSeq Version	XM_005171060.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calmodulin-regulated spectrin-associated protein 3-like (LOC100007608), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171060.3

ORF Insert Sequence:

ATGGCGATGG ACGCGCCCGC GGTGAAGCAG AGCGCGCCGG TACCGGACAT CTGCCCACTG 
GACCGGTACG AGCCGAGCCA AGCCAAGACC CGAGCCAGCC TGAGCTGGCT GCTGAGCCGA 
AGCGGACCTG AAGAGAGCGT GTCGTCAGCG GTGTGTGCGG ACGGCTCAGT GTGTGTGTCC 
CTGCAGCGCC GTCTGCTGTC CGCTGAGCTG TACTGTCGTG TGTTCTCGGG TGTGTGTGTG 
TCCGCTGGGG GCGTGGCTCC GGCAGATCTG CCCTCCCTCC TGCTGCTGCT GGACCGCCTC 
CACCTGACGC CGTCGGCCAA TCAGACGCAG CTCAGCCAGA CGCCCATACA CATGGGGGCT 
CATCTGGCTC TCATTGATTC GCTGATGTCA CTGAGCGCCA TGGAAACGGT TGCCCGGGTG 
AAGATGGCGA AGGAGACGGA TCAGTTCGGC TCAGGAAAGA GCTGGGAAAA CTCTCTGCTC 
TTCTGGATCA ACAAGGTCAA TCAGAAGCTG CGGGACAGTG CTGATGTGGA GGAGAATGTG 
AAGCCAAACA CCTGCACAGA CCTGCAGCCG GTGCAGCCCA CGTGTCCTCA GCGCTGGTAC 
TGGAAGCTGG TCCCTCATGC CATCGCATTT TGTTTGAAGG AGTCTGGGAA TAAACCTCCA 
GTGATCCGTT ACAGGAAAGA CAAGGTTCAG TCTAAGCAGA GTCCGGTGTT CTCTGTGGTG 
TCTGGAGTGA AGGATCTCTC CAGCGGCTGC GCCATCGCTG CTGCGCTTCA CTTCTACTGC 
CCTCAGATAC TGCCGCTGGA GGACGTGTGC TTGAAGGACA CCATGTGTGT AGCAGACAGT 
GTGTATAATC TCCAGCTGAT CCGAGAGTTT TATAGAACTC ACCTGAAGAG CTGCTGCCCC 
CTACAGCTGG AGGATTTACT GTACGCCCCG CCCATTCTGC AGGTGAACAT TATGTGCTTC 
CTTTCTGAGC TGTTTGCCAC ATTCGAGGTG CAGAAACCAG ATTTCGTCAA ACCCATACAC 
ACACTGGACC TAACAGATGC ATCAGATCTG GTGGACTGCA CTAGTCCCAT CAACTGCAAC 
AGTGGCTCCC CATCTTTCCT CCTGAAGCAG TCTCTGCCTC AGTCCACCTC AGTGTCGGGA 
CGGGGATGGA GCAAAAAGCA AATAAGCCGT CCTCTCTCGG CGGTGACCTT CAGCATCCCG 
TTTCCGCTGG ACAGTGATGT TGATGTTGTC ATGGGCAACC CTCTCTTCCG CTCTGTCAGC 
ACGGACAGTC TCACTATGGT AACCAACCCT GACCCTTACA CTCCTCCAGA GGATCTCAGT 
AAACTCATTG GTCATGCTCC TCTAGGAGAG CTGCCTACTA TAGAAGAAGC ATTACAGATC 
GTTCACACTG GCCGTGTGGT ACACAACGAG CCGCGTCTGC GGCCAGAGGG GGCACCATCT 
GGCTTTTACC TCCACTCCCA AAATGAGGGT GGAGCCAAAC TCAGCAGCTC TGCTCCCTGC 
CGGTCAGGGA TTATGTATCG ACCCATTGGA GGAGGGCCTG ATAACAGCGG GAACCACAAA 
CGGCAAGATG GAGGTTCAGA ATGTGCCACG CCTGACTCTC CCAAAACCTC CAGCCCAGCC 
AACGGACCCA AGTCTGGCCG CATGACAAAC TTTGCTGAGC GCAGAAAAAT GATGCCTGAA 
TCCCCAAGCA ATTCCAGGTC CCAGGAGCGG GAACCTTTGT TCAGCCCTGC AGCAATGAGC 
CCTGTTGGGG CAATGGAGGC GCAGGAGCTG GGTGCACGTC TGGAGGAGAA GCGGAAAGCA 
ATTGAAGCTC AAAAGAGGAG GATTGAGGCC ATCTTTACCA AGCACAGGCA AAGGCTGGGC 
AAAACTGCCT TCCTTCAGCT CCAGAAGAAA CAAGAGGGAG AGGTGGAGGA AGATGGACAG 
TCCATCTCTC TGGATGAACG ACTCACACAA ATGGAGCAGC AGCTTCAACA AGTGGAGGAC 
AGAGAGAAGG AGGAGGAGAA AAGAAAGCAG GAAGAGGGGA AGAGAGAAAA GGATGGGGAG 
GAGAAGAAGA GTGATGTAGT AGCTCCACCA AAACTAGAGA AACAGGTGAC CTTTTCTGTT 
GAGGCAAAGA AAGAGGAGGG GAAAGAGCCA CCGTTGGGGG AATATAACGA GGCAGTAGCT 
AAGCTAAGCT CCACCCTGGA GACTCTGCAA AGAGACATGC AGCGCCTTAC AGAACAGCAG 
CAAAGGCTCA TGGGAAAGAA AACTTTGAGT TCTCCAAAAA ACATCCCCTC AAGCAAGACC 
TCAGCATCCA GCAAGGCCTG GATTATTCCA TCTGGTCCCA AATCCCCCGC TACACCACCT 
CATCTTTCTA GAGACTCCAC TCGCATCATT TCACCCTCTG GCTCTCCATC ACGCTCTGGA 
CAACTTTCAG ACACACCGAG ATCCCCTAAA AGCTCTGCAG CATCCACCCC TCGTAGATCC 
CGTAGTTCTG CTCCCAAGAG TCCTAGGCGT CAACGACCCT CCCGCCCCAC GGACCTTGGC 
TTCCAGCCTA TCACACGTGT CCTAACACCG CCGCAGAATG TGGACACCCT TCCCCATTTG 
CGCAGAGTCT CTCCAAGCCA GTGTCAGGTG CAGACCAGCT CATCTCTGCG GCTCGGAGGT 
CCGTACACCC CACTGGACTC TTCACCCCAA CCTGCCCCTG CTCAAGAGAA CACATCAGAG 
TCAGGCTCCA GTGACCACAA CACTCCAATC TTCAGTCTTG AGCTAGAGGC TGGACCTCCG 
TCTGCACTCC CAGCGGAGCT GTTAGGAGGA GCAGGCAGCT CTGGAGCTCC ATCAGAGTGC 
TCGTTTGAAA GTGACTTGCT TTTGAGCAGT GCCCCCGTGA AAGACGAGAC GGAGGGTGCC 
GGAACGATGG GAGATGACAC CGATTTAGAG CAGGGTGCAG AAATATTCTC ATCAGATTCC 
ATGAGTGACC AGACTGAGAG TGAGAGCCGG CTGGGACTTG ATGTGTTTTT TAAGGAGGAG 
GGTCTCTCGG AGGAGGAGAT GGCCCAGAAA AGGGCCCTGC TGTTGGAACG ACAGCAAAGA 
CGAGCACAAG AGCTCAAGAG ACGAGCAAGA CAAGGGCAGG ACCCTGAGAA CAGTCAACAG 
GCTTCCTTGG AAGACCTGCG ACCGCAAACT CCACTGCTAT CACAGTCTCT GCCCACCTTT 
TACACCCCAC CTCCTCATTT GAATGCAACG CCCCCAGGCA CGCCCCAGCG CTGGGGAGCA 
TTCACACGGG CGGAGTATGA ACGGCGCCAT CAGCTGAAGA TAATGGCTGA TCTAGGGAAG 
GTTCTCAAAC AGAAACCAAC AAAGCAAAGT GGCAAAAAGC AGCATGCAAA CAAACCACAT 
AACCACGAGC TCATTCACAC ACGCTCACCT GGGAAGAGCA GCACAGCCAA CCAGAAAGAT 
GGCAGATCTC TACCAGCTGT AAAACCAGCA AGTCGTTTAG AGTCACCCTC AAAGGTGATG 
TCATCAGGAC TGCTGGCCAG TCAGAATGGA GAGAAAGACT GTGAACAAGC CTCCAACGCC 
TCCTCTCCAG CATCACTCCC AGAATACACA GGTCCTAAAT TGTTTAAGGA GCCCAGTTTT 
AAATCCAACA AGTTCATCAT TCACAGTGCG CTCTCACGCT GCTGCCTTGC TGGCAAAGTC 
AACGAATCCC AGAAGAACAA GATCATCGAG GAGATGGAGA AGAGCTCCGC CAGTCACTTT 
CTGATCCTCA TGCGTGATTC CAACTGTCAG TTCCGTGCCA TCTACACCCT AGACGGCCAG 
TCAGAGGAGC TGCACCGACT CTGCGGCGTT GGTCCACGGG TCATTTCCTC ATCAGCTGTG 
GAAGCCATTT ATAAATACAG TTCTGATAGG AAACAGTTCA ATACACTTCC ATCCCGCACT 
CTGAGCATGA GCGTCGACGC TTTCACTATC CCTGGCCAGC TCTGGAACAC TAAGAAACAT 
GGCACGCCCA AAAAAGTAGC CACTCCCAAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100007608 ( XM_005171060.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171117.1 Danio rerio calmodulin-regulated spectrin-associated protein 3-like (LOC100007608), transcript variant X2, mRNA.

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CloneID	ODa06527
Clone ID Related Accession (Same CDS sequence)	XM_005171060.3 , XM_005171060.4
Accession Version	XM_005171060.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	calmodulin-regulated spectrin-associated protein 3-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005171060.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGATGG ACGCGCCCGC GGTGAAGCAG AGCGCGCCGG TACCGGACAT CTGCCCACTG 
GACCGGTACG AGCCGAGCCA AGCCAAGACC CGAGCCAGCC TGAGCTGGCT GCTGAGCCGA 
AGCGGACCTG AAGAGAGCGT GTCGTCAGCG GTGTGTGCGG ACGGCTCAGT GTGTGTGTCC 
CTGCAGCGCC GTCTGCTGTC CGCTGAGCTG TACTGTCGTG TGTTCTCGGG TGTGTGTGTG 
TCCGCTGGGG GCGTGGCTCC GGCAGATCTG CCCTCCCTCC TGCTGCTGCT GGACCGCCTC 
CACCTGACGC CGTCGGCCAA TCAGACGCAG CTCAGCCAGA CGCCCATACA CATGGGGGCT 
CATCTGGCTC TCATTGATTC GCTGATGTCA CTGAGCGCCA TGGAAACGGT TGCCCGGGTG 
AAGATGGCGA AGGAGACGGA TCAGTTCGGC TCAGGAAAGA GCTGGGAAAA CTCTCTGCTC 
TTCTGGATCA ACAAGGTCAA TCAGAAGCTG CGGGACAGTG CTGATGTGGA GGAGAATGTG 
AAGCCAAACA CCTGCACAGA CCTGCAGCCG GTGCAGCCCA CGTGTCCTCA GCGCTGGTAC 
TGGAAGCTGG TCCCTCATGC CATCGCATTT TGTTTGAAGG AGTCTGGGAA TAAACCTCCA 
GTGATCCGTT ACAGGAAAGA CAAGGTTCAG TCTAAGCAGA GTCCGGTGTT CTCTGTGGTG 
TCTGGAGTGA AGGATCTCTC CAGCGGCTGC GCCATCGCTG CTGCGCTTCA CTTCTACTGC 
CCTCAGATAC TGCCGCTGGA GGACGTGTGC TTGAAGGACA CCATGTGTGT AGCAGACAGT 
GTGTATAATC TCCAGCTGAT CCGAGAGTTT TATAGAACTC ACCTGAAGAG CTGCTGCCCC 
CTACAGCTGG AGGATTTACT GTACGCCCCG CCCATTCTGC AGGTGAACAT TATGTGCTTC 
CTTTCTGAGC TGTTTGCCAC ATTCGAGGTG CAGAAACCAG ATTTCGTCAA ACCCATACAC 
ACACTGGACC TAACAGATGC ATCAGATCTG GTGGACTGCA CTAGTCCCAT CAACTGCAAC 
AGTGGCTCCC CATCTTTCCT CCTGAAGCAG TCTCTGCCTC AGTCCACCTC AGTGTCGGGA 
CGGGGATGGA GCAAAAAGCA AATAAGCCGT CCTCTCTCGG CGGTGACCTT CAGCATCCCG 
TTTCCGCTGG ACAGTGATGT TGATGTTGTC ATGGGCAACC CTCTCTTCCG CTCTGTCAGC 
ACGGACAGTC TCACTATGGT AACCAACCCT GACCCTTACA CTCCTCCAGA GGATCTCAGT 
AAACTCATTG GTCATGCTCC TCTAGGAGAG CTGCCTACTA TAGAAGAAGC ATTACAGATC 
GTTCACACTG GCCGTGTGGT ACACAACGAG CCGCGTCTGC GGCCAGAGGG GGCACCATCT 
GGCTTTTACC TCCACTCCCA AAATGAGGGT GGAGCCAAAC TCAGCAGCTC TGCTCCCTGC 
CGGTCAGGGA TTATGTATCG ACCCATTGGA GGAGGGCCTG ATAACAGCGG GAACCACAAA 
CGGCAAGATG GAGGTTCAGA ATGTGCCACG CCTGACTCTC CCAAAACCTC CAGCCCAGCC 
AACGGACCCA AGTCTGGCCG CATGACAAAC TTTGCTGAGC GCAGAAAAAT GATGCCTGAA 
TCCCCAAGCA ATTCCAGGTC CCAGGAGCGG GAACCTTTGT TCAGCCCTGC AGCAATGAGC 
CCTGTTGGGG CAATGGAGGC GCAGGAGCTG GGTGCACGTC TGGAGGAGAA GCGGAAAGCA 
ATTGAAGCTC AAAAGAGGAG GATTGAGGCC ATCTTTACCA AGCACAGGCA AAGGCTGGGC 
AAAACTGCCT TCCTTCAGCT CCAGAAGAAA CAAGAGGGAG AGGTGGAGGA AGATGGACAG 
TCCATCTCTC TGGATGAACG ACTCACACAA ATGGAGCAGC AGCTTCAACA AGTGGAGGAC 
AGAGAGAAGG AGGAGGAGAA AAGAAAGCAG GAAGAGGGGA AGAGAGAAAA GGATGGGGAG 
GAGAAGAAGA GTGATGTAGT AGCTCCACCA AAACTAGAGA AACAGGTGAC CTTTTCTGTT 
GAGGCAAAGA AAGAGGAGGG GAAAGAGCCA CCGTTGGGGG AATATAACGA GGCAGTAGCT 
AAGCTAAGCT CCACCCTGGA GACTCTGCAA AGAGACATGC AGCGCCTTAC AGAACAGCAG 
CAAAGGCTCA TGGGAAAGAA AACTTTGAGT TCTCCAAAAA ACATCCCCTC AAGCAAGACC 
TCAGCATCCA GCAAGGCCTG GATTATTCCA TCTGGTCCCA AATCCCCCGC TACACCACCT 
CATCTTTCTA GAGACTCCAC TCGCATCATT TCACCCTCTG GCTCTCCATC ACGCTCTGGA 
CAACTTTCAG ACACACCGAG ATCCCCTAAA AGCTCTGCAG CATCCACCCC TCGTAGATCC 
CGTAGTTCTG CTCCCAAGAG TCCTAGGCGT CAACGACCCT CCCGCCCCAC GGACCTTGGC 
TTCCAGCCTA TCACACGTGT CCTAACACCG CCGCAGAATG TGGACACCCT TCCCCATTTG 
CGCAGAGTCT CTCCAAGCCA GTGTCAGGTG CAGACCAGCT CATCTCTGCG GCTCGGAGGT 
CCGTACACCC CACTGGACTC TTCACCCCAA CCTGCCCCTG CTCAAGAGAA CACATCAGAG 
TCAGGCTCCA GTGACCACAA CACTCCAATC TTCAGTCTTG AGCTAGAGGC TGGACCTCCG 
TCTGCACTCC CAGCGGAGCT GTTAGGAGGA GCAGGCAGCT CTGGAGCTCC ATCAGAGTGC 
TCGTTTGAAA GTGACTTGCT TTTGAGCAGT GCCCCCGTGA AAGACGAGAC GGAGGGTGCC 
GGAACGATGG GAGATGACAC CGATTTAGAG CAGGGTGCAG AAATATTCTC ATCAGATTCC 
ATGAGTGACC AGACTGAGAG TGAGAGCCGG CTGGGACTTG ATGTGTTTTT TAAGGAGGAG 
GGTCTCTCGG AGGAGGAGAT GGCCCAGAAA AGGGCCCTGC TGTTGGAACG ACAGCAAAGA 
CGAGCACAAG AGCTCAAGAG ACGAGCAAGA CAAGGGCAGG ACCCTGAGAA CAGTCAACAG 
GCTTCCTTGG AAGACCTGCG ACCGCAAACT CCACTGCTAT CACAGTCTCT GCCCACCTTT 
TACACCCCAC CTCCTCATTT GAATGCAACG CCCCCAGGCA CGCCCCAGCG CTGGGGAGCA 
TTCACACGGG CGGAGTATGA ACGGCGCCAT CAGCTGAAGA TAATGGCTGA TCTAGGGAAG 
GTTCTCAAAC AGAAACCAAC AAAGCAAAGT GGCAAAAAGC AGCATGCAAA CAAACCACAT 
AACCACGAGC TCATTCACAC ACGCTCACCT GGGAAGAGCA GCACAGCCAA CCAGAAAGAT 
GGCAGATCTC TACCAGCTGT AAAACCAGCA AGTCGTTTAG AGTCACCCTC AAAGGTGATG 
TCATCAGGAC TGCTGGCCAG TCAGAATGGA GAGAAAGACT GTGAACAAGC CTCCAACGCC 
TCCTCTCCAG CATCACTCCC AGAATACACA GGTCCTAAAT TGTTTAAGGA GCCCAGTTTT 
AAATCCAACA AGTTCATCAT TCACAGTGCG CTCTCACGCT GCTGCCTTGC TGGCAAAGTC 
AACGAATCCC AGAAGAACAA GATCATCGAG GAGATGGAGA AGAGCTCCGC CAGTCACTTT 
CTGATCCTCA TGCGTGATTC CAACTGTCAG TTCCGTGCCA TCTACACCCT AGACGGCCAG 
TCAGAGGAGC TGCACCGACT CTGCGGCGTT GGTCCACGGG TCATTTCCTC ATCAGCTGTG 
GAAGCCATTT ATAAATACAG TTCTGATAGG AAACAGTTCA ATACACTTCC ATCCCGCACT 
CTGAGCATGA GCGTCGACGC TTTCACTATC CCTGGCCAGC TCTGGAACAC TAAGAAACAT 
GGCACGCCCA AAAAAGTAGC CACTCCCAAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171117.1
CDS	179..4171
Translation	MAMDAPAVKQSAPVPDICPLDRYEPSQAKTRASLSWLLSRSGPEESVSSAVCADGSVCVSLQRRLLSAELYCRVFSGVCVSAGGVAPADLPSLLLLLDRLHLTPSANQTQLSQTPIHMGAHLALIDSLMSLSAMETVARVKMAKETDQFGSGKSWENSLLFWINKVNQKLRDSADVEENVKPNTCTDLQPVQPTCPQRWYWKLVPHAIAFCLKESGNKPPVIRYRKDKVQSKQSPVFSVVSGVKDLSSGCAIAAALHFYCPQILPLEDVCLKDTMCVADSVYNLQLIREFYRTHLKSCCPLQLEDLLYAPPILQVNIMCFLSELFATFEVQKPDFVKPIHTLDLTDASDLVDCTSPINCNSGSPSFLLKQSLPQSTSVSGRGWSKKQISRPLSAVTFSIPFPLDSDVDVVMGNPLFRSVSTDSLTMVTNPDPYTPPEDLSKLIGHAPLGELPTIEEALQIVHTGRVVHNEPRLRPEGAPSGFYLHSQNEGGAKLSSSAPCRSGIMYRPIGGGPDNSGNHKRQDGGSECATPDSPKTSSPANGPKSGRMTNFAERRKMMPESPSNSRSQEREPLFSPAAMSPVGAMEAQELGARLEEKRKAIEAQKRRIEAIFTKHRQRLGKTAFLQLQKKQEGEVEEDGQSISLDERLTQMEQQLQQVEDREKEEEKRKQEEGKREKDGEEKKSDVVAPPKLEKQVTFSVEAKKEEGKEPPLGEYNEAVAKLSSTLETLQRDMQRLTEQQQRLMGKKTLSSPKNIPSSKTSASSKAWIIPSGPKSPATPPHLSRDSTRIISPSGSPSRSGQLSDTPRSPKSSAASTPRRSRSSAPKSPRRQRPSRPTDLGFQPITRVLTPPQNVDTLPHLRRVSPSQCQVQTSSSLRLGGPYTPLDSSPQPAPAQENTSESGSSDHNTPIFSLELEAGPPSALPAELLGGAGSSGAPSECSFESDLLLSSAPVKDETEGAGTMGDDTDLEQGAEIFSSDSMSDQTESESRLGLDVFFKEEGLSEEEMAQKRALLLERQQRRAQELKRRARQGQDPENSQQASLEDLRPQTPLLSQSLPTFYTPPPHLNATPPGTPQRWGAFTRAEYERRHQLKIMADLGKVLKQKPTKQSGKKQHANKPHNHELIHTRSPGKSSTANQKDGRSLPAVKPASRLESPSKVMSSGLLASQNGEKDCEQASNASSPASLPEYTGPKLFKEPSFKSNKFIIHSALSRCCLAGKVNESQKNKIIEEMEKSSASHFLILMRDSNCQFRAIYTLDGQSEELHRLCGVGPRVISSSAVEAIYKYSSDRKQFNTLPSRTLSMSVDAFTIPGQLWNTKKHGTPKKVATPK

Target ORF information:

RefSeq Version	XM_005171060.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calmodulin-regulated spectrin-associated protein 3-like (LOC100007608), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171060.4

ORF Insert Sequence:

ATGGCGATGG ACGCGCCCGC GGTGAAGCAG AGCGCGCCGG TACCGGACAT CTGCCCACTG 
GACCGGTACG AGCCGAGCCA AGCCAAGACC CGAGCCAGCC TGAGCTGGCT GCTGAGCCGA 
AGCGGACCTG AAGAGAGCGT GTCGTCAGCG GTGTGTGCGG ACGGCTCAGT GTGTGTGTCC 
CTGCAGCGCC GTCTGCTGTC CGCTGAGCTG TACTGTCGTG TGTTCTCGGG TGTGTGTGTG 
TCCGCTGGGG GCGTGGCTCC GGCAGATCTG CCCTCCCTCC TGCTGCTGCT GGACCGCCTC 
CACCTGACGC CGTCGGCCAA TCAGACGCAG CTCAGCCAGA CGCCCATACA CATGGGGGCT 
CATCTGGCTC TCATTGATTC GCTGATGTCA CTGAGCGCCA TGGAAACGGT TGCCCGGGTG 
AAGATGGCGA AGGAGACGGA TCAGTTCGGC TCAGGAAAGA GCTGGGAAAA CTCTCTGCTC 
TTCTGGATCA ACAAGGTCAA TCAGAAGCTG CGGGACAGTG CTGATGTGGA GGAGAATGTG 
AAGCCAAACA CCTGCACAGA CCTGCAGCCG GTGCAGCCCA CGTGTCCTCA GCGCTGGTAC 
TGGAAGCTGG TCCCTCATGC CATCGCATTT TGTTTGAAGG AGTCTGGGAA TAAACCTCCA 
GTGATCCGTT ACAGGAAAGA CAAGGTTCAG TCTAAGCAGA GTCCGGTGTT CTCTGTGGTG 
TCTGGAGTGA AGGATCTCTC CAGCGGCTGC GCCATCGCTG CTGCGCTTCA CTTCTACTGC 
CCTCAGATAC TGCCGCTGGA GGACGTGTGC TTGAAGGACA CCATGTGTGT AGCAGACAGT 
GTGTATAATC TCCAGCTGAT CCGAGAGTTT TATAGAACTC ACCTGAAGAG CTGCTGCCCC 
CTACAGCTGG AGGATTTACT GTACGCCCCG CCCATTCTGC AGGTGAACAT TATGTGCTTC 
CTTTCTGAGC TGTTTGCCAC ATTCGAGGTG CAGAAACCAG ATTTCGTCAA ACCCATACAC 
ACACTGGACC TAACAGATGC ATCAGATCTG GTGGACTGCA CTAGTCCCAT CAACTGCAAC 
AGTGGCTCCC CATCTTTCCT CCTGAAGCAG TCTCTGCCTC AGTCCACCTC AGTGTCGGGA 
CGGGGATGGA GCAAAAAGCA AATAAGCCGT CCTCTCTCGG CGGTGACCTT CAGCATCCCG 
TTTCCGCTGG ACAGTGATGT TGATGTTGTC ATGGGCAACC CTCTCTTCCG CTCTGTCAGC 
ACGGACAGTC TCACTATGGT AACCAACCCT GACCCTTACA CTCCTCCAGA GGATCTCAGT 
AAACTCATTG GTCATGCTCC TCTAGGAGAG CTGCCTACTA TAGAAGAAGC ATTACAGATC 
GTTCACACTG GCCGTGTGGT ACACAACGAG CCGCGTCTGC GGCCAGAGGG GGCACCATCT 
GGCTTTTACC TCCACTCCCA AAATGAGGGT GGAGCCAAAC TCAGCAGCTC TGCTCCCTGC 
CGGTCAGGGA TTATGTATCG ACCCATTGGA GGAGGGCCTG ATAACAGCGG GAACCACAAA 
CGGCAAGATG GAGGTTCAGA ATGTGCCACG CCTGACTCTC CCAAAACCTC CAGCCCAGCC 
AACGGACCCA AGTCTGGCCG CATGACAAAC TTTGCTGAGC GCAGAAAAAT GATGCCTGAA 
TCCCCAAGCA ATTCCAGGTC CCAGGAGCGG GAACCTTTGT TCAGCCCTGC AGCAATGAGC 
CCTGTTGGGG CAATGGAGGC GCAGGAGCTG GGTGCACGTC TGGAGGAGAA GCGGAAAGCA 
ATTGAAGCTC AAAAGAGGAG GATTGAGGCC ATCTTTACCA AGCACAGGCA AAGGCTGGGC 
AAAACTGCCT TCCTTCAGCT CCAGAAGAAA CAAGAGGGAG AGGTGGAGGA AGATGGACAG 
TCCATCTCTC TGGATGAACG ACTCACACAA ATGGAGCAGC AGCTTCAACA AGTGGAGGAC 
AGAGAGAAGG AGGAGGAGAA AAGAAAGCAG GAAGAGGGGA AGAGAGAAAA GGATGGGGAG 
GAGAAGAAGA GTGATGTAGT AGCTCCACCA AAACTAGAGA AACAGGTGAC CTTTTCTGTT 
GAGGCAAAGA AAGAGGAGGG GAAAGAGCCA CCGTTGGGGG AATATAACGA GGCAGTAGCT 
AAGCTAAGCT CCACCCTGGA GACTCTGCAA AGAGACATGC AGCGCCTTAC AGAACAGCAG 
CAAAGGCTCA TGGGAAAGAA AACTTTGAGT TCTCCAAAAA ACATCCCCTC AAGCAAGACC 
TCAGCATCCA GCAAGGCCTG GATTATTCCA TCTGGTCCCA AATCCCCCGC TACACCACCT 
CATCTTTCTA GAGACTCCAC TCGCATCATT TCACCCTCTG GCTCTCCATC ACGCTCTGGA 
CAACTTTCAG ACACACCGAG ATCCCCTAAA AGCTCTGCAG CATCCACCCC TCGTAGATCC 
CGTAGTTCTG CTCCCAAGAG TCCTAGGCGT CAACGACCCT CCCGCCCCAC GGACCTTGGC 
TTCCAGCCTA TCACACGTGT CCTAACACCG CCGCAGAATG TGGACACCCT TCCCCATTTG 
CGCAGAGTCT CTCCAAGCCA GTGTCAGGTG CAGACCAGCT CATCTCTGCG GCTCGGAGGT 
CCGTACACCC CACTGGACTC TTCACCCCAA CCTGCCCCTG CTCAAGAGAA CACATCAGAG 
TCAGGCTCCA GTGACCACAA CACTCCAATC TTCAGTCTTG AGCTAGAGGC TGGACCTCCG 
TCTGCACTCC CAGCGGAGCT GTTAGGAGGA GCAGGCAGCT CTGGAGCTCC ATCAGAGTGC 
TCGTTTGAAA GTGACTTGCT TTTGAGCAGT GCCCCCGTGA AAGACGAGAC GGAGGGTGCC 
GGAACGATGG GAGATGACAC CGATTTAGAG CAGGGTGCAG AAATATTCTC ATCAGATTCC 
ATGAGTGACC AGACTGAGAG TGAGAGCCGG CTGGGACTTG ATGTGTTTTT TAAGGAGGAG 
GGTCTCTCGG AGGAGGAGAT GGCCCAGAAA AGGGCCCTGC TGTTGGAACG ACAGCAAAGA 
CGAGCACAAG AGCTCAAGAG ACGAGCAAGA CAAGGGCAGG ACCCTGAGAA CAGTCAACAG 
GCTTCCTTGG AAGACCTGCG ACCGCAAACT CCACTGCTAT CACAGTCTCT GCCCACCTTT 
TACACCCCAC CTCCTCATTT GAATGCAACG CCCCCAGGCA CGCCCCAGCG CTGGGGAGCA 
TTCACACGGG CGGAGTATGA ACGGCGCCAT CAGCTGAAGA TAATGGCTGA TCTAGGGAAG 
GTTCTCAAAC AGAAACCAAC AAAGCAAAGT GGCAAAAAGC AGCATGCAAA CAAACCACAT 
AACCACGAGC TCATTCACAC ACGCTCACCT GGGAAGAGCA GCACAGCCAA CCAGAAAGAT 
GGCAGATCTC TACCAGCTGT AAAACCAGCA AGTCGTTTAG AGTCACCCTC AAAGGTGATG 
TCATCAGGAC TGCTGGCCAG TCAGAATGGA GAGAAAGACT GTGAACAAGC CTCCAACGCC 
TCCTCTCCAG CATCACTCCC AGAATACACA GGTCCTAAAT TGTTTAAGGA GCCCAGTTTT 
AAATCCAACA AGTTCATCAT TCACAGTGCG CTCTCACGCT GCTGCCTTGC TGGCAAAGTC 
AACGAATCCC AGAAGAACAA GATCATCGAG GAGATGGAGA AGAGCTCCGC CAGTCACTTT 
CTGATCCTCA TGCGTGATTC CAACTGTCAG TTCCGTGCCA TCTACACCCT AGACGGCCAG 
TCAGAGGAGC TGCACCGACT CTGCGGCGTT GGTCCACGGG TCATTTCCTC ATCAGCTGTG 
GAAGCCATTT ATAAATACAG TTCTGATAGG AAACAGTTCA ATACACTTCC ATCCCGCACT 
CTGAGCATGA GCGTCGACGC TTTCACTATC CCTGGCCAGC TCTGGAACAC TAAGAAACAT 
GGCACGCCCA AAAAAGTAGC CACTCCCAAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100007608 ( XM_005171061.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171118.1 Danio rerio calmodulin-regulated spectrin-associated protein 3-like (LOC100007608), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa06528
Clone ID Related Accession (Same CDS sequence)	XM_005171061.3 , XM_005171061.4
Accession Version	XM_005171061.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3948bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	calmodulin-regulated spectrin-associated protein 3-like isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005171061.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGATGG ACGCGCCCGC GGTGAAGCAG AGCGCGCCGG TACCGGACAT CTGCCCACTG 
GACCGGTACG AGCCGAGCCA AGCCAAGACC CGAGCCAGCC TGAGCTGGCT GCTGAGCCGA 
AGCGGACCTG AAGAGAGCGT GTCGTCAGCG GTGTGTGCGG ACGGCTCAGT GTGTGTGTCC 
CTGCAGCGCC GTCTGCTGTC CGCTGAGCTG TACTGTCGTG TGTTCTCGGG TGTGTGTGTG 
TCCGCTGGGG GCGTGGCTCC GGCAGATCTG CCCTCCCTCC TGCTGCTGCT GGACCGCCTC 
CACCTGACGC CGTCGGCCAA TCAGACGCAG CTCAGCCAGA CGCCCATACA CATGGGGGCT 
CATCTGGCTC TCATTGATTC GCTGATGTCA CTGAGCGCCA TGGAAACGGT TGCCCGGGTG 
AAGATGGCGA AGGAGACGGA TCAGTTCGGC TCAGGAAAGA GCTGGGAAAA CTCTCTGCTC 
TTCTGGATCA ACAAGGTCAA TCAGAAGCTG CGGGACAGTG CTGATGTGGA GGAGAATGTG 
AAGCCAAACA CCTGCACAGA CCTGCAGCCG GTGCAGCCCA CGTGTCCTCA GCGCTGGTAC 
TGGAAGCTGG TCCCTATCCG TTACAGGAAA GACAAGGTTC AGTCTAAGCA GAGTCCGGTG 
TTCTCTGTGG TGTCTGGAGT GAAGGATCTC TCCAGCGGCT GCGCCATCGC TGCTGCGCTT 
CACTTCTACT GCCCTCAGAT ACTGCCGCTG GAGGACGTGT GCTTGAAGGA CACCATGTGT 
GTAGCAGACA GTGTGTATAA TCTCCAGCTG ATCCGAGAGT TTTATAGAAC TCACCTGAAG 
AGCTGCTGCC CCCTACAGCT GGAGGATTTA CTGTACGCCC CGCCCATTCT GCAGGTGAAC 
ATTATGTGCT TCCTTTCTGA GCTGTTTGCC ACATTCGAGG TGCAGAAACC AGATTTCGTC 
AAACCCATAC ACACACTGGA CCTAACAGAT GCATCAGATC TGGTGGACTG CACTAGTCCC 
ATCAACTGCA ACAGTGGCTC CCCATCTTTC CTCCTGAAGC AGTCTCTGCC TCAGTCCACC 
TCAGTGTCGG AAGGACGGGG ATGGAGCAAA AAGCAAATAA GCCGTCCTCT CTCGGCGGTG 
ACCTTCAGCA TCCCGTTTCC GCTGGACAGT GATGTTGATG TTGTCATGGG CAACCCTCTC 
TTCCGCTCTG TCAGCACGGA CAGTCTCACT ATGGTAACCA ACCCTGACCC TTACACTCCT 
CCAGAGGATC TCAGTAAACT CATTGGTCAT GCTCCTCTAG GAGAGCTGCC TACTATAGAA 
GAAGCATTAC AGATCGTTCA CACTGGCCGT GTGGTACACA ACGAGCCGCG TCTGCGGCCA 
GAGGGGGCAC CATCTGGCTT TTACCTCCAC TCCCAAAATG AGGGTGGAGC CAAACTCAGC 
AGCTCTGCTC CCTGCCGGTC AGGGATTATG TATCGACCCA TTGGAGGAGG GCCTGATAAC 
AGCGGGAACC ACAAACGGCA AGATGGAGGT TCAGAATGTG CCACGCCTGA CTCTCCCAAA 
ACCTCCAGCC CAGCCAACGG ACCCAAGTCT GGCCGCATGA CAAACTTTGC TGAGCGCAGA 
AAAATGATGC CTGAATCCCC AAGCAATTCC AGGTCCCAGG AGCGGGAACC TTTGTTCAGC 
CCTGCAGCAA TGAGCCCTGT TGGGGCAATG GAGGCGCAGG AGCTGGGTGC ACGTCTGGAG 
GAGAAGCGGA AAGCAATTGA AGCTCAAAAG AGGAGGATTG AGGCCATCTT TACCAAGCAC 
AGGCAAAGGC TGGGCAAAAC TGCCTTCCTT CAGCTCCAGA AGAAACAAGA GGGAGAGGTG 
GAGGAAGATG GACAGTCCAT CTCTCTGGAT GAACGACTCA CACAAATGGA GCAGCAGCTT 
CAACAAGTGG AGGACAGAGA GAAGGAGGAG GAGAAAAGAA AGCAGGAAGA GGGGAAGAGA 
GAAAAGGATG GGGAGGAGAA GAAGAGTGAT GTAGTAGCTC CACCAAAACT AGAGAAACAG 
GTGACCTTTT CTGTTGAGGC AAAGAAAGAG GAGGGGAAAG AGCCACCGTT GGGGGAATAT 
AACGAGGCAG TAGCTAAGCT AAGCTCCACC CTGGAGACTC TGCAAAGAGA CATGCAGCGC 
CTTACAGAAC AGCAGCAAAG GCTCATGGGA AAGAAAACTT TGAGTTCTCC AAAAAACATC 
CCCTCAAGCA AGACCTCAGC ATCCAGCAAG GCCTGGATTA TTCCATCTGG TCCCAAATCC 
CCCGCTACAC CACCTCATCT TTCTAGAGAC TCCACTCGCA TCATTTCACC CTCTGGCTCT 
CCATCACGCT CTGGACAACT TTCAGACACA CCGAGATCCC CTAAAAGCTC TGCAGCATCC 
ACCCCTCGTA GATCCCGTAG TTCTGCTCCC AAGAGTCCTA GGCGTCAACG ACCCTCCCGC 
CCCACGGACC TTGGCTTCCA GCCTATCACA CGTGTCCTAA CACCGCCGCA GAATGTGGAC 
ACCCTTCCCC ATTTGCGCAG AGTCTCTCCA AGCCAGTGTC AGGTGCAGAC CAGCTCATCT 
CTGCGGCTCG GAGGTCCGTA CACCCCACTG GACTCTTCAC CCCAACCTGC CCCTGCTCAA 
GAGAACACAT CAGAGTCAGG CTCCAGTGAC CACAACACTC CAATCTTCAG TCTTGAGCTA 
GAGGCTGGAC CTCCGTCTGC ACTCCCAGCG GAGCTGTTAG GAGGAGCAGG CAGCTCTGGA 
GCTCCATCAG AGTGCTCGTT TGAAAGTGAC TTGCTTTTGA GCAGTGCCCC CGTGAAAGAC 
GAGACGGAGG GTGCCGGAAC GATGGGAGAT GACACCGATT TAGAGCAGGG TGCAGAAATA 
TTCTCATCAG ATTCCATGAG TGACCAGACT GAGAGTGAGA GCCGGCTGGG ACTTGATGTG 
TTTTTTAAGG AGGAGGGTCT CTCGGAGGAG GAGATGGCCC AGAAAAGGGC CCTGCTGTTG 
GAACGACAGC AAAGACGAGC ACAAGAGCTC AAGAGACGAG CAAGACAAGG GCAGGACCCT 
GAGAACAGTC AACAGGCTTC CTTGGAAGAC CTGCGACCGC AAACTCCACT GCTATCACAG 
TCTCTGCCCA CCTTTTACAC CCCACCTCCT CATTTGAATG CAACGCCCCC AGGCACGCCC 
CAGCGCTGGG GAGCATTCAC ACGGGCGGAG TATGAACGGC GCCATCAGCT GAAGATAATG 
GCTGATCTAG GGAAGGTTCT CAAACAGAAA CCAACAAAGC AAAGTGGCAA AAAGCAGCAT 
GCAAACAAAC CACATAACCA CGAGCTCATT CACACACGCT CACCTGGGAA GAGCAGCACA 
GCCAACCAGA AAGATGGCAG ATCTCTACCA GCTGTAAAAC CAGCAAGTCG TTTAGAGTCA 
CCCTCAAAGG TGATGTCATC AGGACTGCTG GCCAGTCAGA ATGGAGAGAA AGACTGTGAA 
CAAGCCTCCA ACGCCTCCTC TCCAGCATCA CTCCCAGAAT ACACAGGTCC TAAATTGTTT 
AAGGAGCCCA GTTTTAAATC CAACAAGTTC ATCATTCACA GTGCGCTCTC ACGCTGCTGC 
CTTGCTGGCA AAGTCAACGA ATCCCAGAAG AACAAGATCA TCGAGGAGAT GGAGAAGAGC 
TCCGCCAGTC ACTTTCTGAT CCTCATGCGT GATTCCAACT GTCAGTTCCG TGCCATCTAC 
ACCCTAGACG GCCAGTCAGA GGAGCTGCAC CGACTCTGCG GCGTTGGTCC ACGGGTCATT 
TCCTCATCAG CTGTGGAAGC CATTTATAAA TACAGTTCTG ATAGGAAACA GTTCAATACA 
CTTCCATCCC GCACTCTGAG CATGAGCGTC GACGCTTTCA CTATCCCTGG CCAGCTCTGG 
AACACTAAGA AACATGGCAC GCCCAAAAAA GTAGCCACTC CCAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171118.1
CDS	359..4306
Translation	MAMDAPAVKQSAPVPDICPLDRYEPSQAKTRASLSWLLSRSGPEESVSSAVCADGSVCVSLQRRLLSAELYCRVFSGVCVSAGGVAPADLPSLLLLLDRLHLTPSANQTQLSQTPIHMGAHLALIDSLMSLSAMETVARVKMAKETDQFGSGKSWENSLLFWINKVNQKLRDSADVEENVKPNTCTDLQPVQPTCPQRWYWKLVPIRYRKDKVQSKQSPVFSVVSGVKDLSSGCAIAAALHFYCPQILPLEDVCLKDTMCVADSVYNLQLIREFYRTHLKSCCPLQLEDLLYAPPILQVNIMCFLSELFATFEVQKPDFVKPIHTLDLTDASDLVDCTSPINCNSGSPSFLLKQSLPQSTSVSEGRGWSKKQISRPLSAVTFSIPFPLDSDVDVVMGNPLFRSVSTDSLTMVTNPDPYTPPEDLSKLIGHAPLGELPTIEEALQIVHTGRVVHNEPRLRPEGAPSGFYLHSQNEGGAKLSSSAPCRSGIMYRPIGGGPDNSGNHKRQDGGSECATPDSPKTSSPANGPKSGRMTNFAERRKMMPESPSNSRSQEREPLFSPAAMSPVGAMEAQELGARLEEKRKAIEAQKRRIEAIFTKHRQRLGKTAFLQLQKKQEGEVEEDGQSISLDERLTQMEQQLQQVEDREKEEEKRKQEEGKREKDGEEKKSDVVAPPKLEKQVTFSVEAKKEEGKEPPLGEYNEAVAKLSSTLETLQRDMQRLTEQQQRLMGKKTLSSPKNIPSSKTSASSKAWIIPSGPKSPATPPHLSRDSTRIISPSGSPSRSGQLSDTPRSPKSSAASTPRRSRSSAPKSPRRQRPSRPTDLGFQPITRVLTPPQNVDTLPHLRRVSPSQCQVQTSSSLRLGGPYTPLDSSPQPAPAQENTSESGSSDHNTPIFSLELEAGPPSALPAELLGGAGSSGAPSECSFESDLLLSSAPVKDETEGAGTMGDDTDLEQGAEIFSSDSMSDQTESESRLGLDVFFKEEGLSEEEMAQKRALLLERQQRRAQELKRRARQGQDPENSQQASLEDLRPQTPLLSQSLPTFYTPPPHLNATPPGTPQRWGAFTRAEYERRHQLKIMADLGKVLKQKPTKQSGKKQHANKPHNHELIHTRSPGKSSTANQKDGRSLPAVKPASRLESPSKVMSSGLLASQNGEKDCEQASNASSPASLPEYTGPKLFKEPSFKSNKFIIHSALSRCCLAGKVNESQKNKIIEEMEKSSASHFLILMRDSNCQFRAIYTLDGQSEELHRLCGVGPRVISSSAVEAIYKYSSDRKQFNTLPSRTLSMSVDAFTIPGQLWNTKKHGTPKKVATPK

Target ORF information:

RefSeq Version	XM_005171061.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calmodulin-regulated spectrin-associated protein 3-like (LOC100007608), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171061.3

ORF Insert Sequence:

ATGGCGATGG ACGCGCCCGC GGTGAAGCAG AGCGCGCCGG TACCGGACAT CTGCCCACTG 
GACCGGTACG AGCCGAGCCA AGCCAAGACC CGAGCCAGCC TGAGCTGGCT GCTGAGCCGA 
AGCGGACCTG AAGAGAGCGT GTCGTCAGCG GTGTGTGCGG ACGGCTCAGT GTGTGTGTCC 
CTGCAGCGCC GTCTGCTGTC CGCTGAGCTG TACTGTCGTG TGTTCTCGGG TGTGTGTGTG 
TCCGCTGGGG GCGTGGCTCC GGCAGATCTG CCCTCCCTCC TGCTGCTGCT GGACCGCCTC 
CACCTGACGC CGTCGGCCAA TCAGACGCAG CTCAGCCAGA CGCCCATACA CATGGGGGCT 
CATCTGGCTC TCATTGATTC GCTGATGTCA CTGAGCGCCA TGGAAACGGT TGCCCGGGTG 
AAGATGGCGA AGGAGACGGA TCAGTTCGGC TCAGGAAAGA GCTGGGAAAA CTCTCTGCTC 
TTCTGGATCA ACAAGGTCAA TCAGAAGCTG CGGGACAGTG CTGATGTGGA GGAGAATGTG 
AAGCCAAACA CCTGCACAGA CCTGCAGCCG GTGCAGCCCA CGTGTCCTCA GCGCTGGTAC 
TGGAAGCTGG TCCCTATCCG TTACAGGAAA GACAAGGTTC AGTCTAAGCA GAGTCCGGTG 
TTCTCTGTGG TGTCTGGAGT GAAGGATCTC TCCAGCGGCT GCGCCATCGC TGCTGCGCTT 
CACTTCTACT GCCCTCAGAT ACTGCCGCTG GAGGACGTGT GCTTGAAGGA CACCATGTGT 
GTAGCAGACA GTGTGTATAA TCTCCAGCTG ATCCGAGAGT TTTATAGAAC TCACCTGAAG 
AGCTGCTGCC CCCTACAGCT GGAGGATTTA CTGTACGCCC CGCCCATTCT GCAGGTGAAC 
ATTATGTGCT TCCTTTCTGA GCTGTTTGCC ACATTCGAGG TGCAGAAACC AGATTTCGTC 
AAACCCATAC ACACACTGGA CCTAACAGAT GCATCAGATC TGGTGGACTG CACTAGTCCC 
ATCAACTGCA ACAGTGGCTC CCCATCTTTC CTCCTGAAGC AGTCTCTGCC TCAGTCCACC 
TCAGTGTCGG AAGGACGGGG ATGGAGCAAA AAGCAAATAA GCCGTCCTCT CTCGGCGGTG 
ACCTTCAGCA TCCCGTTTCC GCTGGACAGT GATGTTGATG TTGTCATGGG CAACCCTCTC 
TTCCGCTCTG TCAGCACGGA CAGTCTCACT ATGGTAACCA ACCCTGACCC TTACACTCCT 
CCAGAGGATC TCAGTAAACT CATTGGTCAT GCTCCTCTAG GAGAGCTGCC TACTATAGAA 
GAAGCATTAC AGATCGTTCA CACTGGCCGT GTGGTACACA ACGAGCCGCG TCTGCGGCCA 
GAGGGGGCAC CATCTGGCTT TTACCTCCAC TCCCAAAATG AGGGTGGAGC CAAACTCAGC 
AGCTCTGCTC CCTGCCGGTC AGGGATTATG TATCGACCCA TTGGAGGAGG GCCTGATAAC 
AGCGGGAACC ACAAACGGCA AGATGGAGGT TCAGAATGTG CCACGCCTGA CTCTCCCAAA 
ACCTCCAGCC CAGCCAACGG ACCCAAGTCT GGCCGCATGA CAAACTTTGC TGAGCGCAGA 
AAAATGATGC CTGAATCCCC AAGCAATTCC AGGTCCCAGG AGCGGGAACC TTTGTTCAGC 
CCTGCAGCAA TGAGCCCTGT TGGGGCAATG GAGGCGCAGG AGCTGGGTGC ACGTCTGGAG 
GAGAAGCGGA AAGCAATTGA AGCTCAAAAG AGGAGGATTG AGGCCATCTT TACCAAGCAC 
AGGCAAAGGC TGGGCAAAAC TGCCTTCCTT CAGCTCCAGA AGAAACAAGA GGGAGAGGTG 
GAGGAAGATG GACAGTCCAT CTCTCTGGAT GAACGACTCA CACAAATGGA GCAGCAGCTT 
CAACAAGTGG AGGACAGAGA GAAGGAGGAG GAGAAAAGAA AGCAGGAAGA GGGGAAGAGA 
GAAAAGGATG GGGAGGAGAA GAAGAGTGAT GTAGTAGCTC CACCAAAACT AGAGAAACAG 
GTGACCTTTT CTGTTGAGGC AAAGAAAGAG GAGGGGAAAG AGCCACCGTT GGGGGAATAT 
AACGAGGCAG TAGCTAAGCT AAGCTCCACC CTGGAGACTC TGCAAAGAGA CATGCAGCGC 
CTTACAGAAC AGCAGCAAAG GCTCATGGGA AAGAAAACTT TGAGTTCTCC AAAAAACATC 
CCCTCAAGCA AGACCTCAGC ATCCAGCAAG GCCTGGATTA TTCCATCTGG TCCCAAATCC 
CCCGCTACAC CACCTCATCT TTCTAGAGAC TCCACTCGCA TCATTTCACC CTCTGGCTCT 
CCATCACGCT CTGGACAACT TTCAGACACA CCGAGATCCC CTAAAAGCTC TGCAGCATCC 
ACCCCTCGTA GATCCCGTAG TTCTGCTCCC AAGAGTCCTA GGCGTCAACG ACCCTCCCGC 
CCCACGGACC TTGGCTTCCA GCCTATCACA CGTGTCCTAA CACCGCCGCA GAATGTGGAC 
ACCCTTCCCC ATTTGCGCAG AGTCTCTCCA AGCCAGTGTC AGGTGCAGAC CAGCTCATCT 
CTGCGGCTCG GAGGTCCGTA CACCCCACTG GACTCTTCAC CCCAACCTGC CCCTGCTCAA 
GAGAACACAT CAGAGTCAGG CTCCAGTGAC CACAACACTC CAATCTTCAG TCTTGAGCTA 
GAGGCTGGAC CTCCGTCTGC ACTCCCAGCG GAGCTGTTAG GAGGAGCAGG CAGCTCTGGA 
GCTCCATCAG AGTGCTCGTT TGAAAGTGAC TTGCTTTTGA GCAGTGCCCC CGTGAAAGAC 
GAGACGGAGG GTGCCGGAAC GATGGGAGAT GACACCGATT TAGAGCAGGG TGCAGAAATA 
TTCTCATCAG ATTCCATGAG TGACCAGACT GAGAGTGAGA GCCGGCTGGG ACTTGATGTG 
TTTTTTAAGG AGGAGGGTCT CTCGGAGGAG GAGATGGCCC AGAAAAGGGC CCTGCTGTTG 
GAACGACAGC AAAGACGAGC ACAAGAGCTC AAGAGACGAG CAAGACAAGG GCAGGACCCT 
GAGAACAGTC AACAGGCTTC CTTGGAAGAC CTGCGACCGC AAACTCCACT GCTATCACAG 
TCTCTGCCCA CCTTTTACAC CCCACCTCCT CATTTGAATG CAACGCCCCC AGGCACGCCC 
CAGCGCTGGG GAGCATTCAC ACGGGCGGAG TATGAACGGC GCCATCAGCT GAAGATAATG 
GCTGATCTAG GGAAGGTTCT CAAACAGAAA CCAACAAAGC AAAGTGGCAA AAAGCAGCAT 
GCAAACAAAC CACATAACCA CGAGCTCATT CACACACGCT CACCTGGGAA GAGCAGCACA 
GCCAACCAGA AAGATGGCAG ATCTCTACCA GCTGTAAAAC CAGCAAGTCG TTTAGAGTCA 
CCCTCAAAGG TGATGTCATC AGGACTGCTG GCCAGTCAGA ATGGAGAGAA AGACTGTGAA 
CAAGCCTCCA ACGCCTCCTC TCCAGCATCA CTCCCAGAAT ACACAGGTCC TAAATTGTTT 
AAGGAGCCCA GTTTTAAATC CAACAAGTTC ATCATTCACA GTGCGCTCTC ACGCTGCTGC 
CTTGCTGGCA AAGTCAACGA ATCCCAGAAG AACAAGATCA TCGAGGAGAT GGAGAAGAGC 
TCCGCCAGTC ACTTTCTGAT CCTCATGCGT GATTCCAACT GTCAGTTCCG TGCCATCTAC 
ACCCTAGACG GCCAGTCAGA GGAGCTGCAC CGACTCTGCG GCGTTGGTCC ACGGGTCATT 
TCCTCATCAG CTGTGGAAGC CATTTATAAA TACAGTTCTG ATAGGAAACA GTTCAATACA 
CTTCCATCCC GCACTCTGAG CATGAGCGTC GACGCTTTCA CTATCCCTGG CCAGCTCTGG 
AACACTAAGA AACATGGCAC GCCCAAAAAA GTAGCCACTC CCAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100007608 ( XM_005171061.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171118.1 Danio rerio calmodulin-regulated spectrin-associated protein 3-like (LOC100007608), transcript variant X3, mRNA.

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CloneID	ODa06528
Clone ID Related Accession (Same CDS sequence)	XM_005171061.3 , XM_005171061.4
Accession Version	XM_005171061.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3948bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	calmodulin-regulated spectrin-associated protein 3-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005171061.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGATGG ACGCGCCCGC GGTGAAGCAG AGCGCGCCGG TACCGGACAT CTGCCCACTG 
GACCGGTACG AGCCGAGCCA AGCCAAGACC CGAGCCAGCC TGAGCTGGCT GCTGAGCCGA 
AGCGGACCTG AAGAGAGCGT GTCGTCAGCG GTGTGTGCGG ACGGCTCAGT GTGTGTGTCC 
CTGCAGCGCC GTCTGCTGTC CGCTGAGCTG TACTGTCGTG TGTTCTCGGG TGTGTGTGTG 
TCCGCTGGGG GCGTGGCTCC GGCAGATCTG CCCTCCCTCC TGCTGCTGCT GGACCGCCTC 
CACCTGACGC CGTCGGCCAA TCAGACGCAG CTCAGCCAGA CGCCCATACA CATGGGGGCT 
CATCTGGCTC TCATTGATTC GCTGATGTCA CTGAGCGCCA TGGAAACGGT TGCCCGGGTG 
AAGATGGCGA AGGAGACGGA TCAGTTCGGC TCAGGAAAGA GCTGGGAAAA CTCTCTGCTC 
TTCTGGATCA ACAAGGTCAA TCAGAAGCTG CGGGACAGTG CTGATGTGGA GGAGAATGTG 
AAGCCAAACA CCTGCACAGA CCTGCAGCCG GTGCAGCCCA CGTGTCCTCA GCGCTGGTAC 
TGGAAGCTGG TCCCTATCCG TTACAGGAAA GACAAGGTTC AGTCTAAGCA GAGTCCGGTG 
TTCTCTGTGG TGTCTGGAGT GAAGGATCTC TCCAGCGGCT GCGCCATCGC TGCTGCGCTT 
CACTTCTACT GCCCTCAGAT ACTGCCGCTG GAGGACGTGT GCTTGAAGGA CACCATGTGT 
GTAGCAGACA GTGTGTATAA TCTCCAGCTG ATCCGAGAGT TTTATAGAAC TCACCTGAAG 
AGCTGCTGCC CCCTACAGCT GGAGGATTTA CTGTACGCCC CGCCCATTCT GCAGGTGAAC 
ATTATGTGCT TCCTTTCTGA GCTGTTTGCC ACATTCGAGG TGCAGAAACC AGATTTCGTC 
AAACCCATAC ACACACTGGA CCTAACAGAT GCATCAGATC TGGTGGACTG CACTAGTCCC 
ATCAACTGCA ACAGTGGCTC CCCATCTTTC CTCCTGAAGC AGTCTCTGCC TCAGTCCACC 
TCAGTGTCGG AAGGACGGGG ATGGAGCAAA AAGCAAATAA GCCGTCCTCT CTCGGCGGTG 
ACCTTCAGCA TCCCGTTTCC GCTGGACAGT GATGTTGATG TTGTCATGGG CAACCCTCTC 
TTCCGCTCTG TCAGCACGGA CAGTCTCACT ATGGTAACCA ACCCTGACCC TTACACTCCT 
CCAGAGGATC TCAGTAAACT CATTGGTCAT GCTCCTCTAG GAGAGCTGCC TACTATAGAA 
GAAGCATTAC AGATCGTTCA CACTGGCCGT GTGGTACACA ACGAGCCGCG TCTGCGGCCA 
GAGGGGGCAC CATCTGGCTT TTACCTCCAC TCCCAAAATG AGGGTGGAGC CAAACTCAGC 
AGCTCTGCTC CCTGCCGGTC AGGGATTATG TATCGACCCA TTGGAGGAGG GCCTGATAAC 
AGCGGGAACC ACAAACGGCA AGATGGAGGT TCAGAATGTG CCACGCCTGA CTCTCCCAAA 
ACCTCCAGCC CAGCCAACGG ACCCAAGTCT GGCCGCATGA CAAACTTTGC TGAGCGCAGA 
AAAATGATGC CTGAATCCCC AAGCAATTCC AGGTCCCAGG AGCGGGAACC TTTGTTCAGC 
CCTGCAGCAA TGAGCCCTGT TGGGGCAATG GAGGCGCAGG AGCTGGGTGC ACGTCTGGAG 
GAGAAGCGGA AAGCAATTGA AGCTCAAAAG AGGAGGATTG AGGCCATCTT TACCAAGCAC 
AGGCAAAGGC TGGGCAAAAC TGCCTTCCTT CAGCTCCAGA AGAAACAAGA GGGAGAGGTG 
GAGGAAGATG GACAGTCCAT CTCTCTGGAT GAACGACTCA CACAAATGGA GCAGCAGCTT 
CAACAAGTGG AGGACAGAGA GAAGGAGGAG GAGAAAAGAA AGCAGGAAGA GGGGAAGAGA 
GAAAAGGATG GGGAGGAGAA GAAGAGTGAT GTAGTAGCTC CACCAAAACT AGAGAAACAG 
GTGACCTTTT CTGTTGAGGC AAAGAAAGAG GAGGGGAAAG AGCCACCGTT GGGGGAATAT 
AACGAGGCAG TAGCTAAGCT AAGCTCCACC CTGGAGACTC TGCAAAGAGA CATGCAGCGC 
CTTACAGAAC AGCAGCAAAG GCTCATGGGA AAGAAAACTT TGAGTTCTCC AAAAAACATC 
CCCTCAAGCA AGACCTCAGC ATCCAGCAAG GCCTGGATTA TTCCATCTGG TCCCAAATCC 
CCCGCTACAC CACCTCATCT TTCTAGAGAC TCCACTCGCA TCATTTCACC CTCTGGCTCT 
CCATCACGCT CTGGACAACT TTCAGACACA CCGAGATCCC CTAAAAGCTC TGCAGCATCC 
ACCCCTCGTA GATCCCGTAG TTCTGCTCCC AAGAGTCCTA GGCGTCAACG ACCCTCCCGC 
CCCACGGACC TTGGCTTCCA GCCTATCACA CGTGTCCTAA CACCGCCGCA GAATGTGGAC 
ACCCTTCCCC ATTTGCGCAG AGTCTCTCCA AGCCAGTGTC AGGTGCAGAC CAGCTCATCT 
CTGCGGCTCG GAGGTCCGTA CACCCCACTG GACTCTTCAC CCCAACCTGC CCCTGCTCAA 
GAGAACACAT CAGAGTCAGG CTCCAGTGAC CACAACACTC CAATCTTCAG TCTTGAGCTA 
GAGGCTGGAC CTCCGTCTGC ACTCCCAGCG GAGCTGTTAG GAGGAGCAGG CAGCTCTGGA 
GCTCCATCAG AGTGCTCGTT TGAAAGTGAC TTGCTTTTGA GCAGTGCCCC CGTGAAAGAC 
GAGACGGAGG GTGCCGGAAC GATGGGAGAT GACACCGATT TAGAGCAGGG TGCAGAAATA 
TTCTCATCAG ATTCCATGAG TGACCAGACT GAGAGTGAGA GCCGGCTGGG ACTTGATGTG 
TTTTTTAAGG AGGAGGGTCT CTCGGAGGAG GAGATGGCCC AGAAAAGGGC CCTGCTGTTG 
GAACGACAGC AAAGACGAGC ACAAGAGCTC AAGAGACGAG CAAGACAAGG GCAGGACCCT 
GAGAACAGTC AACAGGCTTC CTTGGAAGAC CTGCGACCGC AAACTCCACT GCTATCACAG 
TCTCTGCCCA CCTTTTACAC CCCACCTCCT CATTTGAATG CAACGCCCCC AGGCACGCCC 
CAGCGCTGGG GAGCATTCAC ACGGGCGGAG TATGAACGGC GCCATCAGCT GAAGATAATG 
GCTGATCTAG GGAAGGTTCT CAAACAGAAA CCAACAAAGC AAAGTGGCAA AAAGCAGCAT 
GCAAACAAAC CACATAACCA CGAGCTCATT CACACACGCT CACCTGGGAA GAGCAGCACA 
GCCAACCAGA AAGATGGCAG ATCTCTACCA GCTGTAAAAC CAGCAAGTCG TTTAGAGTCA 
CCCTCAAAGG TGATGTCATC AGGACTGCTG GCCAGTCAGA ATGGAGAGAA AGACTGTGAA 
CAAGCCTCCA ACGCCTCCTC TCCAGCATCA CTCCCAGAAT ACACAGGTCC TAAATTGTTT 
AAGGAGCCCA GTTTTAAATC CAACAAGTTC ATCATTCACA GTGCGCTCTC ACGCTGCTGC 
CTTGCTGGCA AAGTCAACGA ATCCCAGAAG AACAAGATCA TCGAGGAGAT GGAGAAGAGC 
TCCGCCAGTC ACTTTCTGAT CCTCATGCGT GATTCCAACT GTCAGTTCCG TGCCATCTAC 
ACCCTAGACG GCCAGTCAGA GGAGCTGCAC CGACTCTGCG GCGTTGGTCC ACGGGTCATT 
TCCTCATCAG CTGTGGAAGC CATTTATAAA TACAGTTCTG ATAGGAAACA GTTCAATACA 
CTTCCATCCC GCACTCTGAG CATGAGCGTC GACGCTTTCA CTATCCCTGG CCAGCTCTGG 
AACACTAAGA AACATGGCAC GCCCAAAAAA GTAGCCACTC CCAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171118.1
CDS	184..4131
Translation	MAMDAPAVKQSAPVPDICPLDRYEPSQAKTRASLSWLLSRSGPEESVSSAVCADGSVCVSLQRRLLSAELYCRVFSGVCVSAGGVAPADLPSLLLLLDRLHLTPSANQTQLSQTPIHMGAHLALIDSLMSLSAMETVARVKMAKETDQFGSGKSWENSLLFWINKVNQKLRDSADVEENVKPNTCTDLQPVQPTCPQRWYWKLVPIRYRKDKVQSKQSPVFSVVSGVKDLSSGCAIAAALHFYCPQILPLEDVCLKDTMCVADSVYNLQLIREFYRTHLKSCCPLQLEDLLYAPPILQVNIMCFLSELFATFEVQKPDFVKPIHTLDLTDASDLVDCTSPINCNSGSPSFLLKQSLPQSTSVSEGRGWSKKQISRPLSAVTFSIPFPLDSDVDVVMGNPLFRSVSTDSLTMVTNPDPYTPPEDLSKLIGHAPLGELPTIEEALQIVHTGRVVHNEPRLRPEGAPSGFYLHSQNEGGAKLSSSAPCRSGIMYRPIGGGPDNSGNHKRQDGGSECATPDSPKTSSPANGPKSGRMTNFAERRKMMPESPSNSRSQEREPLFSPAAMSPVGAMEAQELGARLEEKRKAIEAQKRRIEAIFTKHRQRLGKTAFLQLQKKQEGEVEEDGQSISLDERLTQMEQQLQQVEDREKEEEKRKQEEGKREKDGEEKKSDVVAPPKLEKQVTFSVEAKKEEGKEPPLGEYNEAVAKLSSTLETLQRDMQRLTEQQQRLMGKKTLSSPKNIPSSKTSASSKAWIIPSGPKSPATPPHLSRDSTRIISPSGSPSRSGQLSDTPRSPKSSAASTPRRSRSSAPKSPRRQRPSRPTDLGFQPITRVLTPPQNVDTLPHLRRVSPSQCQVQTSSSLRLGGPYTPLDSSPQPAPAQENTSESGSSDHNTPIFSLELEAGPPSALPAELLGGAGSSGAPSECSFESDLLLSSAPVKDETEGAGTMGDDTDLEQGAEIFSSDSMSDQTESESRLGLDVFFKEEGLSEEEMAQKRALLLERQQRRAQELKRRARQGQDPENSQQASLEDLRPQTPLLSQSLPTFYTPPPHLNATPPGTPQRWGAFTRAEYERRHQLKIMADLGKVLKQKPTKQSGKKQHANKPHNHELIHTRSPGKSSTANQKDGRSLPAVKPASRLESPSKVMSSGLLASQNGEKDCEQASNASSPASLPEYTGPKLFKEPSFKSNKFIIHSALSRCCLAGKVNESQKNKIIEEMEKSSASHFLILMRDSNCQFRAIYTLDGQSEELHRLCGVGPRVISSSAVEAIYKYSSDRKQFNTLPSRTLSMSVDAFTIPGQLWNTKKHGTPKKVATPK

Target ORF information:

RefSeq Version	XM_005171061.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calmodulin-regulated spectrin-associated protein 3-like (LOC100007608), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171061.4

ORF Insert Sequence:

ATGGCGATGG ACGCGCCCGC GGTGAAGCAG AGCGCGCCGG TACCGGACAT CTGCCCACTG 
GACCGGTACG AGCCGAGCCA AGCCAAGACC CGAGCCAGCC TGAGCTGGCT GCTGAGCCGA 
AGCGGACCTG AAGAGAGCGT GTCGTCAGCG GTGTGTGCGG ACGGCTCAGT GTGTGTGTCC 
CTGCAGCGCC GTCTGCTGTC CGCTGAGCTG TACTGTCGTG TGTTCTCGGG TGTGTGTGTG 
TCCGCTGGGG GCGTGGCTCC GGCAGATCTG CCCTCCCTCC TGCTGCTGCT GGACCGCCTC 
CACCTGACGC CGTCGGCCAA TCAGACGCAG CTCAGCCAGA CGCCCATACA CATGGGGGCT 
CATCTGGCTC TCATTGATTC GCTGATGTCA CTGAGCGCCA TGGAAACGGT TGCCCGGGTG 
AAGATGGCGA AGGAGACGGA TCAGTTCGGC TCAGGAAAGA GCTGGGAAAA CTCTCTGCTC 
TTCTGGATCA ACAAGGTCAA TCAGAAGCTG CGGGACAGTG CTGATGTGGA GGAGAATGTG 
AAGCCAAACA CCTGCACAGA CCTGCAGCCG GTGCAGCCCA CGTGTCCTCA GCGCTGGTAC 
TGGAAGCTGG TCCCTATCCG TTACAGGAAA GACAAGGTTC AGTCTAAGCA GAGTCCGGTG 
TTCTCTGTGG TGTCTGGAGT GAAGGATCTC TCCAGCGGCT GCGCCATCGC TGCTGCGCTT 
CACTTCTACT GCCCTCAGAT ACTGCCGCTG GAGGACGTGT GCTTGAAGGA CACCATGTGT 
GTAGCAGACA GTGTGTATAA TCTCCAGCTG ATCCGAGAGT TTTATAGAAC TCACCTGAAG 
AGCTGCTGCC CCCTACAGCT GGAGGATTTA CTGTACGCCC CGCCCATTCT GCAGGTGAAC 
ATTATGTGCT TCCTTTCTGA GCTGTTTGCC ACATTCGAGG TGCAGAAACC AGATTTCGTC 
AAACCCATAC ACACACTGGA CCTAACAGAT GCATCAGATC TGGTGGACTG CACTAGTCCC 
ATCAACTGCA ACAGTGGCTC CCCATCTTTC CTCCTGAAGC AGTCTCTGCC TCAGTCCACC 
TCAGTGTCGG AAGGACGGGG ATGGAGCAAA AAGCAAATAA GCCGTCCTCT CTCGGCGGTG 
ACCTTCAGCA TCCCGTTTCC GCTGGACAGT GATGTTGATG TTGTCATGGG CAACCCTCTC 
TTCCGCTCTG TCAGCACGGA CAGTCTCACT ATGGTAACCA ACCCTGACCC TTACACTCCT 
CCAGAGGATC TCAGTAAACT CATTGGTCAT GCTCCTCTAG GAGAGCTGCC TACTATAGAA 
GAAGCATTAC AGATCGTTCA CACTGGCCGT GTGGTACACA ACGAGCCGCG TCTGCGGCCA 
GAGGGGGCAC CATCTGGCTT TTACCTCCAC TCCCAAAATG AGGGTGGAGC CAAACTCAGC 
AGCTCTGCTC CCTGCCGGTC AGGGATTATG TATCGACCCA TTGGAGGAGG GCCTGATAAC 
AGCGGGAACC ACAAACGGCA AGATGGAGGT TCAGAATGTG CCACGCCTGA CTCTCCCAAA 
ACCTCCAGCC CAGCCAACGG ACCCAAGTCT GGCCGCATGA CAAACTTTGC TGAGCGCAGA 
AAAATGATGC CTGAATCCCC AAGCAATTCC AGGTCCCAGG AGCGGGAACC TTTGTTCAGC 
CCTGCAGCAA TGAGCCCTGT TGGGGCAATG GAGGCGCAGG AGCTGGGTGC ACGTCTGGAG 
GAGAAGCGGA AAGCAATTGA AGCTCAAAAG AGGAGGATTG AGGCCATCTT TACCAAGCAC 
AGGCAAAGGC TGGGCAAAAC TGCCTTCCTT CAGCTCCAGA AGAAACAAGA GGGAGAGGTG 
GAGGAAGATG GACAGTCCAT CTCTCTGGAT GAACGACTCA CACAAATGGA GCAGCAGCTT 
CAACAAGTGG AGGACAGAGA GAAGGAGGAG GAGAAAAGAA AGCAGGAAGA GGGGAAGAGA 
GAAAAGGATG GGGAGGAGAA GAAGAGTGAT GTAGTAGCTC CACCAAAACT AGAGAAACAG 
GTGACCTTTT CTGTTGAGGC AAAGAAAGAG GAGGGGAAAG AGCCACCGTT GGGGGAATAT 
AACGAGGCAG TAGCTAAGCT AAGCTCCACC CTGGAGACTC TGCAAAGAGA CATGCAGCGC 
CTTACAGAAC AGCAGCAAAG GCTCATGGGA AAGAAAACTT TGAGTTCTCC AAAAAACATC 
CCCTCAAGCA AGACCTCAGC ATCCAGCAAG GCCTGGATTA TTCCATCTGG TCCCAAATCC 
CCCGCTACAC CACCTCATCT TTCTAGAGAC TCCACTCGCA TCATTTCACC CTCTGGCTCT 
CCATCACGCT CTGGACAACT TTCAGACACA CCGAGATCCC CTAAAAGCTC TGCAGCATCC 
ACCCCTCGTA GATCCCGTAG TTCTGCTCCC AAGAGTCCTA GGCGTCAACG ACCCTCCCGC 
CCCACGGACC TTGGCTTCCA GCCTATCACA CGTGTCCTAA CACCGCCGCA GAATGTGGAC 
ACCCTTCCCC ATTTGCGCAG AGTCTCTCCA AGCCAGTGTC AGGTGCAGAC CAGCTCATCT 
CTGCGGCTCG GAGGTCCGTA CACCCCACTG GACTCTTCAC CCCAACCTGC CCCTGCTCAA 
GAGAACACAT CAGAGTCAGG CTCCAGTGAC CACAACACTC CAATCTTCAG TCTTGAGCTA 
GAGGCTGGAC CTCCGTCTGC ACTCCCAGCG GAGCTGTTAG GAGGAGCAGG CAGCTCTGGA 
GCTCCATCAG AGTGCTCGTT TGAAAGTGAC TTGCTTTTGA GCAGTGCCCC CGTGAAAGAC 
GAGACGGAGG GTGCCGGAAC GATGGGAGAT GACACCGATT TAGAGCAGGG TGCAGAAATA 
TTCTCATCAG ATTCCATGAG TGACCAGACT GAGAGTGAGA GCCGGCTGGG ACTTGATGTG 
TTTTTTAAGG AGGAGGGTCT CTCGGAGGAG GAGATGGCCC AGAAAAGGGC CCTGCTGTTG 
GAACGACAGC AAAGACGAGC ACAAGAGCTC AAGAGACGAG CAAGACAAGG GCAGGACCCT 
GAGAACAGTC AACAGGCTTC CTTGGAAGAC CTGCGACCGC AAACTCCACT GCTATCACAG 
TCTCTGCCCA CCTTTTACAC CCCACCTCCT CATTTGAATG CAACGCCCCC AGGCACGCCC 
CAGCGCTGGG GAGCATTCAC ACGGGCGGAG TATGAACGGC GCCATCAGCT GAAGATAATG 
GCTGATCTAG GGAAGGTTCT CAAACAGAAA CCAACAAAGC AAAGTGGCAA AAAGCAGCAT 
GCAAACAAAC CACATAACCA CGAGCTCATT CACACACGCT CACCTGGGAA GAGCAGCACA 
GCCAACCAGA AAGATGGCAG ATCTCTACCA GCTGTAAAAC CAGCAAGTCG TTTAGAGTCA 
CCCTCAAAGG TGATGTCATC AGGACTGCTG GCCAGTCAGA ATGGAGAGAA AGACTGTGAA 
CAAGCCTCCA ACGCCTCCTC TCCAGCATCA CTCCCAGAAT ACACAGGTCC TAAATTGTTT 
AAGGAGCCCA GTTTTAAATC CAACAAGTTC ATCATTCACA GTGCGCTCTC ACGCTGCTGC 
CTTGCTGGCA AAGTCAACGA ATCCCAGAAG AACAAGATCA TCGAGGAGAT GGAGAAGAGC 
TCCGCCAGTC ACTTTCTGAT CCTCATGCGT GATTCCAACT GTCAGTTCCG TGCCATCTAC 
ACCCTAGACG GCCAGTCAGA GGAGCTGCAC CGACTCTGCG GCGTTGGTCC ACGGGTCATT 
TCCTCATCAG CTGTGGAAGC CATTTATAAA TACAGTTCTG ATAGGAAACA GTTCAATACA 
CTTCCATCCC GCACTCTGAG CATGAGCGTC GACGCTTTCA CTATCCCTGG CCAGCTCTGG 
AACACTAAGA AACATGGCAC GCCCAAAAAA GTAGCCACTC CCAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC100007608 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム

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