il6st cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	il6st
Entrez Gene ID	100006778
Full Name	interleukin 6 signal transducer
Synonyms	gp130
General protein information	Preferred Names interleukin 6 signal transducer Names interleukin-6 receptor subunit beta
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005155489.4	XP_005155546.1	interleukin-6 receptor subunit beta isoform X1
XM_005155489.3	XP_005155546.1	interleukin-6 receptor subunit beta isoform X1
NM_001365484.1	NP_001352413.1	interleukin-6 receptor subunit beta isoform 2 precursor
NM_001113504.1	NP_001106976.1	interleukin-6 receptor subunit beta precursor
NM_001113504.2	NP_001106976.1	interleukin-6 receptor subunit beta isoform 1 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	IL6ST	NP_002175.2
Pan troglodytes (chimpanzee)	IL6ST	XP_001144416.1
Rattus norvegicus (Norway rat)	Il6st	NP_001008725.2
Danio rerio (zebrafish)	il6st	NP_001106976.1
Xenopus tropicalis (tropical clawed frog)	il6st	XP_002940276.2
	IL6ST	XP_001100601.1
Canis lupus familiaris (dog)	IL6ST	XP_005617454.1
Mus musculus (house mouse)	Il6st	NP_034690.3
Bos taurus (cattle)	IL6ST	XP_002696368.3
Gallus gallus (chicken)	IL6ST	NP_990202.1

Gene Ontology
Bibliography

Related articles in PubMed

Inflammatory response and blood hypercoagulable state induced by low level co-exposure with silica nanoparticles and benzo[a]pyrene in zebrafish (Danio rerio) embryos.
Duan J, Yu Y, Li Y, Wang Y, Sun Z
Chemosphere151152-62(2016 May)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The expression of IL-6, IL-6R and gp130 transcripts were detected very early, increased during the first week of life and were predominantly expressed in the head, epidermis and neuromasts of the anterior and posterior lateral line system.
Title: Characterisation, expression and ontogeny of interleukin-6 and its receptors in zebrafish (Danio rerio).

The following il6st gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the il6st cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa00466	NM_001113504.2 Latest version!	Danio rerio interleukin 6 signal transducer (il6st), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa15795	NM_001365484.1 Latest version!	Danio rerio interleukin 6 signal transducer (il6st), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa15795	XM_005155489.4 Latest version!	Danio rerio interleukin 6 signal transducer (il6st), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
View Old Accession Versions >>
ODa15795	XM_005155489.3	Danio rerio interleukin 6 signal transducer (il6st), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa00466	NM_001113504.1	Danio rerio interleukin 6 signal transducer (il6st), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

il6st ( NM_001113504.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001106976.1 Danio rerio interleukin 6 signal transducer (il6st), mRNA.

Price & Availability↑

CloneID	ODa00466
Clone ID Related Accession (Same CDS sequence)	NM_001113504.1 , NM_001113504.2
Accession Version	NM_001113504.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-05
Organism	Danio rerio(zebrafish)
Product	interleukin-6 receptor subunit beta precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BN000730.1. On Jan 16, 2008 this sequence version replaced XM_001345398.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BN000730.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGACACTT CACATCATTG GCTGTTGGTC TTGATGCTCT TGCATTATGC AAAAGGTTCC 
AACAGTAGCT GTGTATTAAA AATTCTTCCA GACTCCAAGA CTAAGATTCC TGTTGAGTTG 
GAAAGAAACT TCACAGCGAC ATGCCACCTC CAGGGTGGAT CTGAATTTAC CTCAAATGAC 
ATCAAATGGT CTTTTAGCAA CAAAGAAACT ACAGACATTT TATCAAAGAC ATTTTATCAC 
AAAATAAATG AAACCTCCGT CAGTGTTACA GTTAACCTCA GCAAAAACAT GGGTCATATG 
TTGATGTGTG AAGCCTCCAG AGAGTCCTTA ACATACATGG GAAAATGTGC CTACGGAATC 
TACCTTGATG CAGGATACCC TCCGATGAAA CCAAAGAATC TGACATGCAT TGCTTTGCAA 
AGGAAGAAGG ATATTTCTCC TGACCTAAAT TGTTATTGGG AACCAGGATT GAGAGACCCA 
CTGCTTGCCA CCAATTATAC TCTTTATGCC CAAGTAGGAC AAGATTTATA TAACAGCACC 
TGTAAACAGA GTCTTGCAAG AAGTTGCACC GTCAACCTGA GCACTTATCC TGTTCATATG 
ACTTTGAAAG TCTGGGTTGA AGTGAAAAAT TCACTGGGGT CAGAACGGTC AGACGAACAC 
GAGTACGATT CCTTGAGTCT TGTGAAACCA AATCCACCAA ATGTCGAAGT TAAAGTTGAA 
GAGGACTTCC CTACATCTTT TGTGGTTAAA TGGGAGTATG ACATTGAAGA CACAACTATG 
CGATTCTCCA GTGCCATTAG ATACTGCCAA GTAGGGTCAA GTGATTGGAA AAAGGTGCCT 
GAGAACTACA CCAATGTGCA CATTACGTCG TATCGACTGC AGTCCCTAGA GCCTTACACT 
GAGTATGTGG TACAGATGCG ATCCATCGGA GAGCACTACA CCATCTGGAG TGAATGGAGC 
TCCAATGTTA CAGTTCGCAC TCCAGAAGCC AGGGAATTCC CACTGCCATT TTTGAAGTGC 
GTTGCACTTT GTGAAGTGGT CAATGCAAGT TTATATGAAC AGACCCTTGA CCTGTCAATT 
TTGACTAAGC CTCCTGTGAA AGCTAATGGA AAAATGTGTA ACTATTCCCT GATCATCAGT 
CAAGATAACC AGGCTTTTGA AAGTCACGTT ATAAAAGCTG ATCAGCTCAA CCAGAGGTAT 
TCACTGGAAG TGCCACCAGA AGAAAATGCC AGTATTGAAA TAACTGCATT CAATTCAGCC 
GGAGGGTCTC CCAAAACTAT TTTGTTCATT CCAAGATCAA ATGACGAGTT TCTTGGCGTG 
GCGAGCATGA GGTGGTGGGT TCATGATGGA AAGCTTCATG TGGAATGGGG TCCTTTGAAG 
CAGCATCCTC CTCATGGCAT TCATATCTCA GAATATCTGC TGGAGTGGGT GAATGAAAAA 
GATGTTCAGC CTCAGTGGCA GAGGGTTCCT AAAAATGTCA ACACCACCAT ACTGAACGGT 
AATTTAGCCA AATACAAGCA GTACAACATA TCAGTCTATC CAATCTACAA GTTCTATTAT 
GATGGTCGAT GGCATTTCCA AGCAGGAACA CCAGTAACCA AGCCAGCCTA CGTTCAGCAA 
GGAGCACCAG AGAAAGGCCC TACTGTCACA GATACCATTT CCAAGAAAAA TAGTGTTACA 
CTGATATGGG AGGAAATTCC ACTGGACCAC CGGTATGGAT TCATCCAAAA CTACACCATA 
TTCTACAGGA CCGGCAACGC TGAGTGGCAA TCTGTGCTAG TGGCCGCTAA CATCCACTCG 
TATGTATTGA CTGATCTGGT CAGTGAACGC GACTATGTGG TTCACATCAT GGCCTCGACG 
GAAGCTGGCT CCAAAAACGG CACCGATTTT AATTTCAAAA CAATGAAGTT CGCCGATGGA 
GAGGTGGAGA TGATTGTAGT GCTGGTGTGC CTCGGCTTCC TGTTCCTGAG CGTCTTCACC 
ATAATGCTCT GCCTTCGCAA GAGAGAAGTG ATTAAGAAGC TGTTATGGCC GCAAGTCCCG 
GATCCGTCTG ACAGCTCTAT TGCCCACTGG TCACCTGATT TCCCTGTAAA GACCGAAATG 
CCCAAGGAAG ACGTCAGCGT GGTGGAAGTG GACGTGTTTG ACGGCAAGTC TTTGTGCGAA 
GAAGATAAAG TTGTTCTGCC GCTAAAGAAG GACAAGTACC TCTCCGAGGA GCACAGCAGC 
GGTATTGGAG GCTCGTCCTG CATGTCTTCG CCCCGCCACA GTGTTTCCGA CAGCGATGAG 
GGCGACTCGG GCCAGACCAC GGCCAGCACC GTCCAGTACT CGTCAGTGGT GGCCAGCGGA 
TACAAGGGCC AAACCCCAAA CCATCAGCCT CCAACGTTCG CCCGCTCCGA GTCCACACAA 
CCGCTGCTGG ACTGCGAGGA GCATCCAGAG AATCATCCCG GCGAGAGGAA CTCTTACTTT 
AGACGGGGCC GAGAGCTGGA GCCGCAGAGT ATCGATGAGC CTGTTTTCAG CGCGGTGCAG 
GAGGAAGAAG CATCTCCCGT TGTGGATGAT GCGCCGGCGT TAAGTTACAT GCCGCAGCAG 
AGCGGGTATC GACCGCAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001106976.1
CDS	1..2601
Translation	MDTSHHWLLVLMLLHYAKGSNSSCVLKILPDSKTKIPVELERNFTATCHLQGGSEFTSNDIKWSFSNKETTDILSKTFYHKINETSVSVTVNLSKNMGHMLMCEASRESLTYMGKCAYGIYLDAGYPPMKPKNLTCIALQRKKDISPDLNCYWEPGLRDPLLATNYTLYAQVGQDLYNSTCKQSLARSCTVNLSTYPVHMTLKVWVEVKNSLGSERSDEHEYDSLSLVKPNPPNVEVKVEEDFPTSFVVKWEYDIEDTTMRFSSAIRYCQVGSSDWKKVPENYTNVHITSYRLQSLEPYTEYVVQMRSIGEHYTIWSEWSSNVTVRTPEAREFPLPFLKCVALCEVVNASLYEQTLDLSILTKPPVKANGKMCNYSLIISQDNQAFESHVIKADQLNQRYSLEVPPEENASIEITAFNSAGGSPKTILFIPRSNDEFLGVASMRWWVHDGKLHVEWGPLKQHPPHGIHISEYLLEWVNEKDVQPQWQRVPKNVNTTILNGNLAKYKQYNISVYPIYKFYYDGRWHFQAGTPVTKPAYVQQGAPEKGPTVTDTISKKNSVTLIWEEIPLDHRYGFIQNYTIFYRTGNAEWQSVLVAANIHSYVLTDLVSERDYVVHIMASTEAGSKNGTDFNFKTMKFADGEVEMIVVLVCLGFLFLSVFTIMLCLRKREVIKKLLWPQVPDPSDSSIAHWSPDFPVKTEMPKEDVSVVEVDVFDGKSLCEEDKVVLPLKKDKYLSEEHSSGIGGSSCMSSPRHSVSDSDEGDSGQTTASTVQYSSVVASGYKGQTPNHQPPTFARSESTQPLLDCEEHPENHPGERNSYFRRGRELEPQSIDEPVFSAVQEEEASPVVDDAPALSYMPQQSGYRPQ

Target ORF information:

RefSeq Version	NM_001113504.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio interleukin 6 signal transducer (il6st), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001113504.1

ORF Insert Sequence:

ATGGACACTT CACATCATTG GCTGTTGGTC TTGATGCTCT TGCATTATGC AAAAGGTTCC 
AACAGTAGCT GTGTATTAAA AATTCTTCCA GACTCCAAGA CTAAGATTCC TGTTGAGTTG 
GAAAGAAACT TCACAGCGAC ATGCCACCTC CAGGGTGGAT CTGAATTTAC CTCAAATGAC 
ATCAAATGGT CTTTTAGCAA CAAAGAAACT ACAGACATTT TATCAAAGAC ATTTTATCAC 
AAAATAAATG AAACCTCCGT CAGTGTTACA GTTAACCTCA GCAAAAACAT GGGTCATATG 
TTGATGTGTG AAGCCTCCAG AGAGTCCTTA ACATACATGG GAAAATGTGC CTACGGAATC 
TACCTTGATG CAGGATACCC TCCGATGAAA CCAAAGAATC TGACATGCAT TGCTTTGCAA 
AGGAAGAAGG ATATTTCTCC TGACCTAAAT TGTTATTGGG AACCAGGATT GAGAGACCCA 
CTGCTTGCCA CCAATTATAC TCTTTATGCC CAAGTAGGAC AAGATTTATA TAACAGCACC 
TGTAAACAGA GTCTTGCAAG AAGTTGCACC GTCAACCTGA GCACTTATCC TGTTCATATG 
ACTTTGAAAG TCTGGGTTGA AGTGAAAAAT TCACTGGGGT CAGAACGGTC AGACGAACAC 
GAGTACGATT CCTTGAGTCT TGTGAAACCA AATCCACCAA ATGTCGAAGT TAAAGTTGAA 
GAGGACTTCC CTACATCTTT TGTGGTTAAA TGGGAGTATG ACATTGAAGA CACAACTATG 
CGATTCTCCA GTGCCATTAG ATACTGCCAA GTAGGGTCAA GTGATTGGAA AAAGGTGCCT 
GAGAACTACA CCAATGTGCA CATTACGTCG TATCGACTGC AGTCCCTAGA GCCTTACACT 
GAGTATGTGG TACAGATGCG ATCCATCGGA GAGCACTACA CCATCTGGAG TGAATGGAGC 
TCCAATGTTA CAGTTCGCAC TCCAGAAGCC AGGGAATTCC CACTGCCATT TTTGAAGTGC 
GTTGCACTTT GTGAAGTGGT CAATGCAAGT TTATATGAAC AGACCCTTGA CCTGTCAATT 
TTGACTAAGC CTCCTGTGAA AGCTAATGGA AAAATGTGTA ACTATTCCCT GATCATCAGT 
CAAGATAACC AGGCTTTTGA AAGTCACGTT ATAAAAGCTG ATCAGCTCAA CCAGAGGTAT 
TCACTGGAAG TGCCACCAGA AGAAAATGCC AGTATTGAAA TAACTGCATT CAATTCAGCC 
GGAGGGTCTC CCAAAACTAT TTTGTTCATT CCAAGATCAA ATGACGAGTT TCTTGGCGTG 
GCGAGCATGA GGTGGTGGGT TCATGATGGA AAGCTTCATG TGGAATGGGG TCCTTTGAAG 
CAGCATCCTC CTCATGGCAT TCATATCTCA GAATATCTGC TGGAGTGGGT GAATGAAAAA 
GATGTTCAGC CTCAGTGGCA GAGGGTTCCT AAAAATGTCA ACACCACCAT ACTGAACGGT 
AATTTAGCCA AATACAAGCA GTACAACATA TCAGTCTATC CAATCTACAA GTTCTATTAT 
GATGGTCGAT GGCATTTCCA AGCAGGAACA CCAGTAACCA AGCCAGCCTA CGTTCAGCAA 
GGAGCACCAG AGAAAGGCCC TACTGTCACA GATACCATTT CCAAGAAAAA TAGTGTTACA 
CTGATATGGG AGGAAATTCC ACTGGACCAC CGGTATGGAT TCATCCAAAA CTACACCATA 
TTCTACAGGA CCGGCAACGC TGAGTGGCAA TCTGTGCTAG TGGCCGCTAA CATCCACTCG 
TATGTATTGA CTGATCTGGT CAGTGAACGC GACTATGTGG TTCACATCAT GGCCTCGACG 
GAAGCTGGCT CCAAAAACGG CACCGATTTT AATTTCAAAA CAATGAAGTT CGCCGATGGA 
GAGGTGGAGA TGATTGTAGT GCTGGTGTGC CTCGGCTTCC TGTTCCTGAG CGTCTTCACC 
ATAATGCTCT GCCTTCGCAA GAGAGAAGTG ATTAAGAAGC TGTTATGGCC GCAAGTCCCG 
GATCCGTCTG ACAGCTCTAT TGCCCACTGG TCACCTGATT TCCCTGTAAA GACCGAAATG 
CCCAAGGAAG ACGTCAGCGT GGTGGAAGTG GACGTGTTTG ACGGCAAGTC TTTGTGCGAA 
GAAGATAAAG TTGTTCTGCC GCTAAAGAAG GACAAGTACC TCTCCGAGGA GCACAGCAGC 
GGTATTGGAG GCTCGTCCTG CATGTCTTCG CCCCGCCACA GTGTTTCCGA CAGCGATGAG 
GGCGACTCGG GCCAGACCAC GGCCAGCACC GTCCAGTACT CGTCAGTGGT GGCCAGCGGA 
TACAAGGGCC AAACCCCAAA CCATCAGCCT CCAACGTTCG CCCGCTCCGA GTCCACACAA 
CCGCTGCTGG ACTGCGAGGA GCATCCAGAG AATCATCCCG GCGAGAGGAA CTCTTACTTT 
AGACGGGGCC GAGAGCTGGA GCCGCAGAGT ATCGATGAGC CTGTTTTCAG CGCGGTGCAG 
GAGGAAGAAG CATCTCCCGT TGTGGATGAT GCGCCGGCGT TAAGTTACAT GCCGCAGCAG 
AGCGGGTATC GACCGCAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

il6st ( NM_001113504.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001106976.1 Danio rerio interleukin 6 signal transducer (il6st), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ODa00466
Clone ID Related Accession (Same CDS sequence)	NM_001113504.1 , NM_001113504.2
Accession Version	NM_001113504.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-21
Organism	Danio rerio(zebrafish)
Product	interleukin-6 receptor subunit beta isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX321872.10. On Aug 30, 2018 this sequence version replaced NM_001113504.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BN000730.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGACACTT CACATCATTG GCTGTTGGTC TTGATGCTCT TGCATTATGC AAAAGGTTCC 
AACAGTAGCT GTGTATTAAA AATTCTTCCA GACTCCAAGA CTAAGATTCC TGTTGAGTTG 
GAAAGAAACT TCACAGCGAC ATGCCACCTC CAGGGTGGAT CTGAATTTAC CTCAAATGAC 
ATCAAATGGT CTTTTAGCAA CAAAGAAACT ACAGACATTT TATCAAAGAC ATTTTATCAC 
AAAATAAATG AAACCTCCGT CAGTGTTACA GTTAACCTCA GCAAAAACAT GGGTCATATG 
TTGATGTGTG AAGCCTCCAG AGAGTCCTTA ACATACATGG GAAAATGTGC CTACGGAATC 
TACCTTGATG CAGGATACCC TCCGATGAAA CCAAAGAATC TGACATGCAT TGCTTTGCAA 
AGGAAGAAGG ATATTTCTCC TGACCTAAAT TGTTATTGGG AACCAGGATT GAGAGACCCA 
CTGCTTGCCA CCAATTATAC TCTTTATGCC CAAGTAGGAC AAGATTTATA TAACAGCACC 
TGTAAACAGA GTCTTGCAAG AAGTTGCACC GTCAACCTGA GCACTTATCC TGTTCATATG 
ACTTTGAAAG TCTGGGTTGA AGTGAAAAAT TCACTGGGGT CAGAACGGTC AGACGAACAC 
GAGTACGATT CCTTGAGTCT TGTGAAACCA AATCCACCAA ATGTCGAAGT TAAAGTTGAA 
GAGGACTTCC CTACATCTTT TGTGGTTAAA TGGGAGTATG ACATTGAAGA CACAACTATG 
CGATTCTCCA GTGCCATTAG ATACTGCCAA GTAGGGTCAA GTGATTGGAA AAAGGTGCCT 
GAGAACTACA CCAATGTGCA CATTACGTCG TATCGACTGC AGTCCCTAGA GCCTTACACT 
GAGTATGTGG TACAGATGCG ATCCATCGGA GAGCACTACA CCATCTGGAG TGAATGGAGC 
TCCAATGTTA CAGTTCGCAC TCCAGAAGCC AGGGAATTCC CACTGCCATT TTTGAAGTGC 
GTTGCACTTT GTGAAGTGGT CAATGCAAGT TTATATGAAC AGACCCTTGA CCTGTCAATT 
TTGACTAAGC CTCCTGTGAA AGCTAATGGA AAAATGTGTA ACTATTCCCT GATCATCAGT 
CAAGATAACC AGGCTTTTGA AAGTCACGTT ATAAAAGCTG ATCAGCTCAA CCAGAGGTAT 
TCACTGGAAG TGCCACCAGA AGAAAATGCC AGTATTGAAA TAACTGCATT CAATTCAGCC 
GGAGGGTCTC CCAAAACTAT TTTGTTCATT CCAAGATCAA ATGACGAGTT TCTTGGCGTG 
GCGAGCATGA GGTGGTGGGT TCATGATGGA AAGCTTCATG TGGAATGGGG TCCTTTGAAG 
CAGCATCCTC CTCATGGCAT TCATATCTCA GAATATCTGC TGGAGTGGGT GAATGAAAAA 
GATGTTCAGC CTCAGTGGCA GAGGGTTCCT AAAAATGTCA ACACCACCAT ACTGAACGGT 
AATTTAGCCA AATACAAGCA GTACAACATA TCAGTCTATC CAATCTACAA GTTCTATTAT 
GATGGTCGAT GGCATTTCCA AGCAGGAACA CCAGTAACCA AGCCAGCCTA CGTTCAGCAA 
GGAGCACCAG AGAAAGGCCC TACTGTCACA GATACCATTT CCAAGAAAAA TAGTGTTACA 
CTGATATGGG AGGAAATTCC ACTGGACCAC CGGTATGGAT TCATCCAAAA CTACACCATA 
TTCTACAGGA CCGGCAACGC TGAGTGGCAA TCTGTGCTAG TGGCCGCTAA CATCCACTCG 
TATGTATTGA CTGATCTGGT CAGTGAACGC GACTATGTGG TTCACATCAT GGCCTCGACG 
GAAGCTGGCT CCAAAAACGG CACCGATTTT AATTTCAAAA CAATGAAGTT CGCCGATGGA 
GAGGTGGAGA TGATTGTAGT GCTGGTGTGC CTCGGCTTCC TGTTCCTGAG CGTCTTCACC 
ATAATGCTCT GCCTTCGCAA GAGAGAAGTG ATTAAGAAGC TGTTATGGCC GCAAGTCCCG 
GATCCGTCTG ACAGCTCTAT TGCCCACTGG TCACCTGATT TCCCTGTAAA GACCGAAATG 
CCCAAGGAAG ACGTCAGCGT GGTGGAAGTG GACGTGTTTG ACGGCAAGTC TTTGTGCGAA 
GAAGATAAAG TTGTTCTGCC GCTAAAGAAG GACAAGTACC TCTCCGAGGA GCACAGCAGC 
GGTATTGGAG GCTCGTCCTG CATGTCTTCG CCCCGCCACA GTGTTTCCGA CAGCGATGAG 
GGCGACTCGG GCCAGACCAC GGCCAGCACC GTCCAGTACT CGTCAGTGGT GGCCAGCGGA 
TACAAGGGCC AAACCCCAAA CCATCAGCCT CCAACGTTCG CCCGCTCCGA GTCCACACAA 
CCGCTGCTGG ACTGCGAGGA GCATCCAGAG AATCATCCCG GCGAGAGGAA CTCTTACTTT 
AGACGGGGCC GAGAGCTGGA GCCGCAGAGT ATCGATGAGC CTGTTTTCAG CGCGGTGCAG 
GAGGAAGAAG CATCTCCCGT TGTGGATGAT GCGCCGGCGT TAAGTTACAT GCCGCAGCAG 
AGCGGGTATC GACCGCAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001106976.1
CDS	78..2678
Translation	MDTSHHWLLVLMLLHYAKGSNSSCVLKILPDSKTKIPVELERNFTATCHLQGGSEFTSNDIKWSFSNKETTDILSKTFYHKINETSVSVTVNLSKNMGHMLMCEASRESLTYMGKCAYGIYLDAGYPPMKPKNLTCIALQRKKDISPDLNCYWEPGLRDPLLATNYTLYAQVGQDLYNSTCKQSLARSCTVNLSTYPVHMTLKVWVEVKNSLGSERSDEHEYDSLSLVKPNPPNVEVKVEEDFPTSFVVKWEYDIEDTTMRFSSAIRYCQVGSSDWKKVPENYTNVHITSYRLQSLEPYTEYVVQMRSIGEHYTIWSEWSSNVTVRTPEAREFPLPFLKCVALCEVVNASLYEQTLDLSILTKPPVKANGKMCNYSLIISQDNQAFESHVIKADQLNQRYSLEVPPEENASIEITAFNSAGGSPKTILFIPRSNDEFLGVASMRWWVHDGKLHVEWGPLKQHPPHGIHISEYLLEWVNEKDVQPQWQRVPKNVNTTILNGNLAKYKQYNISVYPIYKFYYDGRWHFQAGTPVTKPAYVQQGAPEKGPTVTDTISKKNSVTLIWEEIPLDHRYGFIQNYTIFYRTGNAEWQSVLVAANIHSYVLTDLVSERDYVVHIMASTEAGSKNGTDFNFKTMKFADGEVEMIVVLVCLGFLFLSVFTIMLCLRKREVIKKLLWPQVPDPSDSSIAHWSPDFPVKTEMPKEDVSVVEVDVFDGKSLCEEDKVVLPLKKDKYLSEEHSSGIGGSSCMSSPRHSVSDSDEGDSGQTTASTVQYSSVVASGYKGQTPNHQPPTFARSESTQPLLDCEEHPENHPGERNSYFRRGRELEPQSIDEPVFSAVQEEEASPVVDDAPALSYMPQQSGYRPQ

Target ORF information:

RefSeq Version	NM_001113504.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio interleukin 6 signal transducer (il6st), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001113504.2

ORF Insert Sequence:

ATGGACACTT CACATCATTG GCTGTTGGTC TTGATGCTCT TGCATTATGC AAAAGGTTCC 
AACAGTAGCT GTGTATTAAA AATTCTTCCA GACTCCAAGA CTAAGATTCC TGTTGAGTTG 
GAAAGAAACT TCACAGCGAC ATGCCACCTC CAGGGTGGAT CTGAATTTAC CTCAAATGAC 
ATCAAATGGT CTTTTAGCAA CAAAGAAACT ACAGACATTT TATCAAAGAC ATTTTATCAC 
AAAATAAATG AAACCTCCGT CAGTGTTACA GTTAACCTCA GCAAAAACAT GGGTCATATG 
TTGATGTGTG AAGCCTCCAG AGAGTCCTTA ACATACATGG GAAAATGTGC CTACGGAATC 
TACCTTGATG CAGGATACCC TCCGATGAAA CCAAAGAATC TGACATGCAT TGCTTTGCAA 
AGGAAGAAGG ATATTTCTCC TGACCTAAAT TGTTATTGGG AACCAGGATT GAGAGACCCA 
CTGCTTGCCA CCAATTATAC TCTTTATGCC CAAGTAGGAC AAGATTTATA TAACAGCACC 
TGTAAACAGA GTCTTGCAAG AAGTTGCACC GTCAACCTGA GCACTTATCC TGTTCATATG 
ACTTTGAAAG TCTGGGTTGA AGTGAAAAAT TCACTGGGGT CAGAACGGTC AGACGAACAC 
GAGTACGATT CCTTGAGTCT TGTGAAACCA AATCCACCAA ATGTCGAAGT TAAAGTTGAA 
GAGGACTTCC CTACATCTTT TGTGGTTAAA TGGGAGTATG ACATTGAAGA CACAACTATG 
CGATTCTCCA GTGCCATTAG ATACTGCCAA GTAGGGTCAA GTGATTGGAA AAAGGTGCCT 
GAGAACTACA CCAATGTGCA CATTACGTCG TATCGACTGC AGTCCCTAGA GCCTTACACT 
GAGTATGTGG TACAGATGCG ATCCATCGGA GAGCACTACA CCATCTGGAG TGAATGGAGC 
TCCAATGTTA CAGTTCGCAC TCCAGAAGCC AGGGAATTCC CACTGCCATT TTTGAAGTGC 
GTTGCACTTT GTGAAGTGGT CAATGCAAGT TTATATGAAC AGACCCTTGA CCTGTCAATT 
TTGACTAAGC CTCCTGTGAA AGCTAATGGA AAAATGTGTA ACTATTCCCT GATCATCAGT 
CAAGATAACC AGGCTTTTGA AAGTCACGTT ATAAAAGCTG ATCAGCTCAA CCAGAGGTAT 
TCACTGGAAG TGCCACCAGA AGAAAATGCC AGTATTGAAA TAACTGCATT CAATTCAGCC 
GGAGGGTCTC CCAAAACTAT TTTGTTCATT CCAAGATCAA ATGACGAGTT TCTTGGCGTG 
GCGAGCATGA GGTGGTGGGT TCATGATGGA AAGCTTCATG TGGAATGGGG TCCTTTGAAG 
CAGCATCCTC CTCATGGCAT TCATATCTCA GAATATCTGC TGGAGTGGGT GAATGAAAAA 
GATGTTCAGC CTCAGTGGCA GAGGGTTCCT AAAAATGTCA ACACCACCAT ACTGAACGGT 
AATTTAGCCA AATACAAGCA GTACAACATA TCAGTCTATC CAATCTACAA GTTCTATTAT 
GATGGTCGAT GGCATTTCCA AGCAGGAACA CCAGTAACCA AGCCAGCCTA CGTTCAGCAA 
GGAGCACCAG AGAAAGGCCC TACTGTCACA GATACCATTT CCAAGAAAAA TAGTGTTACA 
CTGATATGGG AGGAAATTCC ACTGGACCAC CGGTATGGAT TCATCCAAAA CTACACCATA 
TTCTACAGGA CCGGCAACGC TGAGTGGCAA TCTGTGCTAG TGGCCGCTAA CATCCACTCG 
TATGTATTGA CTGATCTGGT CAGTGAACGC GACTATGTGG TTCACATCAT GGCCTCGACG 
GAAGCTGGCT CCAAAAACGG CACCGATTTT AATTTCAAAA CAATGAAGTT CGCCGATGGA 
GAGGTGGAGA TGATTGTAGT GCTGGTGTGC CTCGGCTTCC TGTTCCTGAG CGTCTTCACC 
ATAATGCTCT GCCTTCGCAA GAGAGAAGTG ATTAAGAAGC TGTTATGGCC GCAAGTCCCG 
GATCCGTCTG ACAGCTCTAT TGCCCACTGG TCACCTGATT TCCCTGTAAA GACCGAAATG 
CCCAAGGAAG ACGTCAGCGT GGTGGAAGTG GACGTGTTTG ACGGCAAGTC TTTGTGCGAA 
GAAGATAAAG TTGTTCTGCC GCTAAAGAAG GACAAGTACC TCTCCGAGGA GCACAGCAGC 
GGTATTGGAG GCTCGTCCTG CATGTCTTCG CCCCGCCACA GTGTTTCCGA CAGCGATGAG 
GGCGACTCGG GCCAGACCAC GGCCAGCACC GTCCAGTACT CGTCAGTGGT GGCCAGCGGA 
TACAAGGGCC AAACCCCAAA CCATCAGCCT CCAACGTTCG CCCGCTCCGA GTCCACACAA 
CCGCTGCTGG ACTGCGAGGA GCATCCAGAG AATCATCCCG GCGAGAGGAA CTCTTACTTT 
AGACGGGGCC GAGAGCTGGA GCCGCAGAGT ATCGATGAGC CTGTTTTCAG CGCGGTGCAG 
GAGGAAGAAG CATCTCCCGT TGTGGATGAT GCGCCGGCGT TAAGTTACAT GCCGCAGCAG 
AGCGGGTATC GACCGCAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

il6st ( NM_001365484.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001352413.1 Danio rerio interleukin 6 signal transducer (il6st), transcript variant 2, mRNA.

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CloneID	ODa15795
Clone ID Related Accession (Same CDS sequence)	XM_005155489.4 , XM_005155489.3 , NM_001365484.1
Accession Version	NM_001365484.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2580bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-21
Organism	Danio rerio(zebrafish)
Product	interleukin-6 receptor subunit beta isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX321872.10. On Aug 30, 2018 this sequence version replaced XM_005155489.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB018216.1, GDQH01003941.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGACACTT CACATCATTG GCTGTTGGTC TTGATGCTCT TGCATTATGC AAAAGGTTCC 
AACAGTAGCT GTGTATTAAA AATTCTTCCA GACTCCAAGA CTAAGATTCC TGTTGAGTTG 
GAAAGAAACT TCACAGCGAC ATGCCACCTC CAGGGTGGAT CTGAATTTAC CTCAAATGAC 
ATCAAATGGT CTTTTAGCAA CAAAGAAACT ACAGACATTT TATCAAAGAC ATTTTATCAC 
AAAATAAATG AAACCTCCGT CAGTGTTACA GTTAACCTCA GCAAAAACAT GGGTCATATG 
TTGATGTGTG AAGCCTCCAG AGAGTCCTTA ACATACATGG GAAAATGTGC CTACGGAATC 
TACCTTGATG CAGGATACCC TCCGATGAAA CCAAAGAATC TGACATGCAT TGCTTTGCAA 
AGGAAGAAGG ATATTTCTCC TGACCTAAAT TGTTATTGGG AACCAGGATT GAGAGACCCA 
CTGCTTGCCA CCAATTATAC TCTTTATGCC CAAGTAGGAC AAGATTTATA TAACAGCACC 
TGTAAACAGA GTCTTGCAAG AAGTTGCACC GTCAACCTGA GCACTTATCC TGTTCATATG 
ACTTTGAAAG TCTGGGTTGA AGTGAAAAAT TCACTGGGGT CAGAACGGTC AGACGAACAC 
GAGTACGATT CCTTGAGTCT TGTGAAACCA AATCCACCAA ATGTCGAAGT TAAAGTTGAA 
GAGGACTTCC CTACATCTTT TGTGGTTAAA TGGGAGTATG ACATTGAAGA CACAACTATG 
CGATTCTCCA GTGCCATTAG ATACTGCCAA GTAGGGTCAA GTGATTGGAA AAAGGTGCCT 
GAGAACTACA CCAATGTGCA CATTACGTCG TATCGACTGC AGTCCCTAGA GCCTTACACT 
GAGTATGTGG TACAGATGCG ATCCATCGGA GAGCACTACA CCATCTGGAG TGAATGGAGC 
TCCAATGTTA CAGTTCGCAC TCCAGAAGCC AAGCCAGCAA GTCCACCAGA CCTGTGGAGA 
ATCATCCAGC ACACGCCTGG GAAAAGAAAC GTCACATTAA TGTGGAAGCC TCCTGTGAAA 
GCTAATGGAA AAATGTGTAA CTATTCCCTG ATCATCAGTC AAGATAACCA GGCTTTTGAA 
AGTCACGTTA TAAAAGCTGA TCAGCTCAAC CAGAGGTATT CACTGGAAGT GCCACCAGAA 
GAAAATGCCA GTATTGAAAT AACTGCATTC AATTCAGCCG GAGGGTCTCC CAAAACTATT 
TTGTTCATTC CAAGATCAAA TGACGAGTTT CTTGGCGTGG CGAGCATGAG GTGGTGGGTT 
CATGATGGAA AGCTTCATGT GGAATGGGGT CCTTTGAAGC AGCATCCTCC TCATGGCATT 
CATATCTCAG AATATCTGCT GGAGTGGGTG AATGAAAAAG ATGTTCAGCC TCAGTGGCAG 
AGGGTTCCTA AAAATGTCAA CACCACCATA CTGAACGGTA ATTTAGCCAA ATACAAGCAG 
TACAACATAT CAGTCTATCC AATCTACAAG TTCTATTATG ATGGTCGATG GCATTTCCAA 
GCAGGAACAC CAGTAACCAA GCCAGCCTAC GTTCAGCAAG GAGCACCAGA GAAAGGCCCT 
ACTGTCACAG ATACCATTTC CAAGAAAAAT AGTGTTACAC TGATATGGGA GGAAATTCCA 
CTGGACCACC GGTATGGATT CATCCAAAAC TACACCATAT TCTACAGGAC CGGCAACGCT 
GAGTGGCAAT CTGTGCTAGT GGCCGCTAAC ATCCACTCGT ATGTATTGAC TGATCTGGTC 
AGTGAACGCG ACTATGTGGT TCACATCATG GCCTCGACGG AAGCTGGCTC CAAAAACGGC 
ACCGATTTTA ATTTCAAAAC AATGAAGTTC GCCGATGGAG AGGTGGAGAT GATTGTAGTG 
CTGGTGTGCC TCGGCTTCCT GTTCCTGAGC GTCTTCACCA TAATGCTCTG CCTTCGCAAG 
AGAGAAGTGA TTAAGAAGCT GTTATGGCCG CAAGTCCCGG ATCCGTCTGA CAGCTCTATT 
GCCCACTGGT CACCTGATTT CCCTGTAAAG ACCGAAATGC CCAAGGAAGA CGTCAGCGTG 
GTGGAAGTGG ACGTGTTTGA CGGCAAGTCT TTGTGCGAAG AAGATAAAGT TGTTCTGCCG 
CTAAAGAAGG ACAAGTACCT CTCCGAGGAG CACAGCAGCG GTATTGGAGG CTCGTCCTGC 
ATGTCTTCGC CCCGCCACAG TGTTTCCGAC AGCGATGAGG GCGACTCGGG CCAGACCACG 
GCCAGCACCG TCCAGTACTC GTCAGTGGTG GCCAGCGGAT ACAAGGGCCA AACCCCAAAC 
CATCAGCCTC CAACGTTCGC CCGCTCCGAG TCCACACAAC CGCTGCTGGA CTGCGAGGAG 
CATCCAGAGA ATCATCCCGG CGAGAGGAAC TCTTACTTTA GACGGGGCCG AGAGCTGGAG 
CCGCAGAGTA TCGATGAGCC TGTTTTCAGC GCGGTGCAGG AGGAAGAAGC ATCTCCCGTT 
GTGGATGATG CGCCGGCGTT AAGTTACATG CCGCAGCAGA GCGGGTATCG ACCGCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001352413.1
CDS	78..2657
Translation	MDTSHHWLLVLMLLHYAKGSNSSCVLKILPDSKTKIPVELERNFTATCHLQGGSEFTSNDIKWSFSNKETTDILSKTFYHKINETSVSVTVNLSKNMGHMLMCEASRESLTYMGKCAYGIYLDAGYPPMKPKNLTCIALQRKKDISPDLNCYWEPGLRDPLLATNYTLYAQVGQDLYNSTCKQSLARSCTVNLSTYPVHMTLKVWVEVKNSLGSERSDEHEYDSLSLVKPNPPNVEVKVEEDFPTSFVVKWEYDIEDTTMRFSSAIRYCQVGSSDWKKVPENYTNVHITSYRLQSLEPYTEYVVQMRSIGEHYTIWSEWSSNVTVRTPEAKPASPPDLWRIIQHTPGKRNVTLMWKPPVKANGKMCNYSLIISQDNQAFESHVIKADQLNQRYSLEVPPEENASIEITAFNSAGGSPKTILFIPRSNDEFLGVASMRWWVHDGKLHVEWGPLKQHPPHGIHISEYLLEWVNEKDVQPQWQRVPKNVNTTILNGNLAKYKQYNISVYPIYKFYYDGRWHFQAGTPVTKPAYVQQGAPEKGPTVTDTISKKNSVTLIWEEIPLDHRYGFIQNYTIFYRTGNAEWQSVLVAANIHSYVLTDLVSERDYVVHIMASTEAGSKNGTDFNFKTMKFADGEVEMIVVLVCLGFLFLSVFTIMLCLRKREVIKKLLWPQVPDPSDSSIAHWSPDFPVKTEMPKEDVSVVEVDVFDGKSLCEEDKVVLPLKKDKYLSEEHSSGIGGSSCMSSPRHSVSDSDEGDSGQTTASTVQYSSVVASGYKGQTPNHQPPTFARSESTQPLLDCEEHPENHPGERNSYFRRGRELEPQSIDEPVFSAVQEEEASPVVDDAPALSYMPQQSGYRPQ

Target ORF information:

RefSeq Version	NM_001365484.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio interleukin 6 signal transducer (il6st), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001365484.1

ORF Insert Sequence:

ATGGACACTT CACATCATTG GCTGTTGGTC TTGATGCTCT TGCATTATGC AAAAGGTTCC 
AACAGTAGCT GTGTATTAAA AATTCTTCCA GACTCCAAGA CTAAGATTCC TGTTGAGTTG 
GAAAGAAACT TCACAGCGAC ATGCCACCTC CAGGGTGGAT CTGAATTTAC CTCAAATGAC 
ATCAAATGGT CTTTTAGCAA CAAAGAAACT ACAGACATTT TATCAAAGAC ATTTTATCAC 
AAAATAAATG AAACCTCCGT CAGTGTTACA GTTAACCTCA GCAAAAACAT GGGTCATATG 
TTGATGTGTG AAGCCTCCAG AGAGTCCTTA ACATACATGG GAAAATGTGC CTACGGAATC 
TACCTTGATG CAGGATACCC TCCGATGAAA CCAAAGAATC TGACATGCAT TGCTTTGCAA 
AGGAAGAAGG ATATTTCTCC TGACCTAAAT TGTTATTGGG AACCAGGATT GAGAGACCCA 
CTGCTTGCCA CCAATTATAC TCTTTATGCC CAAGTAGGAC AAGATTTATA TAACAGCACC 
TGTAAACAGA GTCTTGCAAG AAGTTGCACC GTCAACCTGA GCACTTATCC TGTTCATATG 
ACTTTGAAAG TCTGGGTTGA AGTGAAAAAT TCACTGGGGT CAGAACGGTC AGACGAACAC 
GAGTACGATT CCTTGAGTCT TGTGAAACCA AATCCACCAA ATGTCGAAGT TAAAGTTGAA 
GAGGACTTCC CTACATCTTT TGTGGTTAAA TGGGAGTATG ACATTGAAGA CACAACTATG 
CGATTCTCCA GTGCCATTAG ATACTGCCAA GTAGGGTCAA GTGATTGGAA AAAGGTGCCT 
GAGAACTACA CCAATGTGCA CATTACGTCG TATCGACTGC AGTCCCTAGA GCCTTACACT 
GAGTATGTGG TACAGATGCG ATCCATCGGA GAGCACTACA CCATCTGGAG TGAATGGAGC 
TCCAATGTTA CAGTTCGCAC TCCAGAAGCC AAGCCAGCAA GTCCACCAGA CCTGTGGAGA 
ATCATCCAGC ACACGCCTGG GAAAAGAAAC GTCACATTAA TGTGGAAGCC TCCTGTGAAA 
GCTAATGGAA AAATGTGTAA CTATTCCCTG ATCATCAGTC AAGATAACCA GGCTTTTGAA 
AGTCACGTTA TAAAAGCTGA TCAGCTCAAC CAGAGGTATT CACTGGAAGT GCCACCAGAA 
GAAAATGCCA GTATTGAAAT AACTGCATTC AATTCAGCCG GAGGGTCTCC CAAAACTATT 
TTGTTCATTC CAAGATCAAA TGACGAGTTT CTTGGCGTGG CGAGCATGAG GTGGTGGGTT 
CATGATGGAA AGCTTCATGT GGAATGGGGT CCTTTGAAGC AGCATCCTCC TCATGGCATT 
CATATCTCAG AATATCTGCT GGAGTGGGTG AATGAAAAAG ATGTTCAGCC TCAGTGGCAG 
AGGGTTCCTA AAAATGTCAA CACCACCATA CTGAACGGTA ATTTAGCCAA ATACAAGCAG 
TACAACATAT CAGTCTATCC AATCTACAAG TTCTATTATG ATGGTCGATG GCATTTCCAA 
GCAGGAACAC CAGTAACCAA GCCAGCCTAC GTTCAGCAAG GAGCACCAGA GAAAGGCCCT 
ACTGTCACAG ATACCATTTC CAAGAAAAAT AGTGTTACAC TGATATGGGA GGAAATTCCA 
CTGGACCACC GGTATGGATT CATCCAAAAC TACACCATAT TCTACAGGAC CGGCAACGCT 
GAGTGGCAAT CTGTGCTAGT GGCCGCTAAC ATCCACTCGT ATGTATTGAC TGATCTGGTC 
AGTGAACGCG ACTATGTGGT TCACATCATG GCCTCGACGG AAGCTGGCTC CAAAAACGGC 
ACCGATTTTA ATTTCAAAAC AATGAAGTTC GCCGATGGAG AGGTGGAGAT GATTGTAGTG 
CTGGTGTGCC TCGGCTTCCT GTTCCTGAGC GTCTTCACCA TAATGCTCTG CCTTCGCAAG 
AGAGAAGTGA TTAAGAAGCT GTTATGGCCG CAAGTCCCGG ATCCGTCTGA CAGCTCTATT 
GCCCACTGGT CACCTGATTT CCCTGTAAAG ACCGAAATGC CCAAGGAAGA CGTCAGCGTG 
GTGGAAGTGG ACGTGTTTGA CGGCAAGTCT TTGTGCGAAG AAGATAAAGT TGTTCTGCCG 
CTAAAGAAGG ACAAGTACCT CTCCGAGGAG CACAGCAGCG GTATTGGAGG CTCGTCCTGC 
ATGTCTTCGC CCCGCCACAG TGTTTCCGAC AGCGATGAGG GCGACTCGGG CCAGACCACG 
GCCAGCACCG TCCAGTACTC GTCAGTGGTG GCCAGCGGAT ACAAGGGCCA AACCCCAAAC 
CATCAGCCTC CAACGTTCGC CCGCTCCGAG TCCACACAAC CGCTGCTGGA CTGCGAGGAG 
CATCCAGAGA ATCATCCCGG CGAGAGGAAC TCTTACTTTA GACGGGGCCG AGAGCTGGAG 
CCGCAGAGTA TCGATGAGCC TGTTTTCAGC GCGGTGCAGG AGGAAGAAGC ATCTCCCGTT 
GTGGATGATG CGCCGGCGTT AAGTTACATG CCGCAGCAGA GCGGGTATCG ACCGCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

il6st ( XM_005155489.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155546.1 Danio rerio interleukin 6 signal transducer (il6st), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa15795
Clone ID Related Accession (Same CDS sequence)	XM_005155489.4 , XM_005155489.3 , NM_001365484.1
Accession Version	XM_005155489.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2580bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	interleukin-6 receptor subunit beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005155489.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACTT CACATCATTG GCTGTTGGTC TTGATGCTCT TGCATTATGC AAAAGGTTCC 
AACAGTAGCT GTGTATTAAA AATTCTTCCA GACTCCAAGA CTAAGATTCC TGTTGAGTTG 
GAAAGAAACT TCACAGCGAC ATGCCACCTC CAGGGTGGAT CTGAATTTAC CTCAAATGAC 
ATCAAATGGT CTTTTAGCAA CAAAGAAACT ACAGACATTT TATCAAAGAC ATTTTATCAC 
AAAATAAATG AAACCTCCGT CAGTGTTACA GTTAACCTCA GCAAAAACAT GGGTCATATG 
TTGATGTGTG AAGCCTCCAG AGAGTCCTTA ACATACATGG GAAAATGTGC CTACGGAATC 
TACCTTGATG CAGGATACCC TCCGATGAAA CCAAAGAATC TGACATGCAT TGCTTTGCAA 
AGGAAGAAGG ATATTTCTCC TGACCTAAAT TGTTATTGGG AACCAGGATT GAGAGACCCA 
CTGCTTGCCA CCAATTATAC TCTTTATGCC CAAGTAGGAC AAGATTTATA TAACAGCACC 
TGTAAACAGA GTCTTGCAAG AAGTTGCACC GTCAACCTGA GCACTTATCC TGTTCATATG 
ACTTTGAAAG TCTGGGTTGA AGTGAAAAAT TCACTGGGGT CAGAACGGTC AGACGAACAC 
GAGTACGATT CCTTGAGTCT TGTGAAACCA AATCCACCAA ATGTCGAAGT TAAAGTTGAA 
GAGGACTTCC CTACATCTTT TGTGGTTAAA TGGGAGTATG ACATTGAAGA CACAACTATG 
CGATTCTCCA GTGCCATTAG ATACTGCCAA GTAGGGTCAA GTGATTGGAA AAAGGTGCCT 
GAGAACTACA CCAATGTGCA CATTACGTCG TATCGACTGC AGTCCCTAGA GCCTTACACT 
GAGTATGTGG TACAGATGCG ATCCATCGGA GAGCACTACA CCATCTGGAG TGAATGGAGC 
TCCAATGTTA CAGTTCGCAC TCCAGAAGCC AAGCCAGCAA GTCCACCAGA CCTGTGGAGA 
ATCATCCAGC ACACGCCTGG GAAAAGAAAC GTCACATTAA TGTGGAAGCC TCCTGTGAAA 
GCTAATGGAA AAATGTGTAA CTATTCCCTG ATCATCAGTC AAGATAACCA GGCTTTTGAA 
AGTCACGTTA TAAAAGCTGA TCAGCTCAAC CAGAGGTATT CACTGGAAGT GCCACCAGAA 
GAAAATGCCA GTATTGAAAT AACTGCATTC AATTCAGCCG GAGGGTCTCC CAAAACTATT 
TTGTTCATTC CAAGATCAAA TGACGAGTTT CTTGGCGTGG CGAGCATGAG GTGGTGGGTT 
CATGATGGAA AGCTTCATGT GGAATGGGGT CCTTTGAAGC AGCATCCTCC TCATGGCATT 
CATATCTCAG AATATCTGCT GGAGTGGGTG AATGAAAAAG ATGTTCAGCC TCAGTGGCAG 
AGGGTTCCTA AAAATGTCAA CACCACCATA CTGAACGGTA ATTTAGCCAA ATACAAGCAG 
TACAACATAT CAGTCTATCC AATCTACAAG TTCTATTATG ATGGTCGATG GCATTTCCAA 
GCAGGAACAC CAGTAACCAA GCCAGCCTAC GTTCAGCAAG GAGCACCAGA GAAAGGCCCT 
ACTGTCACAG ATACCATTTC CAAGAAAAAT AGTGTTACAC TGATATGGGA GGAAATTCCA 
CTGGACCACC GGTATGGATT CATCCAAAAC TACACCATAT TCTACAGGAC CGGCAACGCT 
GAGTGGCAAT CTGTGCTAGT GGCCGCTAAC ATCCACTCGT ATGTATTGAC TGATCTGGTC 
AGTGAACGCG ACTATGTGGT TCACATCATG GCCTCGACGG AAGCTGGCTC CAAAAACGGC 
ACCGATTTTA ATTTCAAAAC AATGAAGTTC GCCGATGGAG AGGTGGAGAT GATTGTAGTG 
CTGGTGTGCC TCGGCTTCCT GTTCCTGAGC GTCTTCACCA TAATGCTCTG CCTTCGCAAG 
AGAGAAGTGA TTAAGAAGCT GTTATGGCCG CAAGTCCCGG ATCCGTCTGA CAGCTCTATT 
GCCCACTGGT CACCTGATTT CCCTGTAAAG ACCGAAATGC CCAAGGAAGA CGTCAGCGTG 
GTGGAAGTGG ACGTGTTTGA CGGCAAGTCT TTGTGCGAAG AAGATAAAGT TGTTCTGCCG 
CTAAAGAAGG ACAAGTACCT CTCCGAGGAG CACAGCAGCG GTATTGGAGG CTCGTCCTGC 
ATGTCTTCGC CCCGCCACAG TGTTTCCGAC AGCGATGAGG GCGACTCGGG CCAGACCACG 
GCCAGCACCG TCCAGTACTC GTCAGTGGTG GCCAGCGGAT ACAAGGGCCA AACCCCAAAC 
CATCAGCCTC CAACGTTCGC CCGCTCCGAG TCCACACAAC CGCTGCTGGA CTGCGAGGAG 
CATCCAGAGA ATCATCCCGG CGAGAGGAAC TCTTACTTTA GACGGGGCCG AGAGCTGGAG 
CCGCAGAGTA TCGATGAGCC TGTTTTCAGC GCGGTGCAGG AGGAAGAAGC ATCTCCCGTT 
GTGGATGATG CGCCGGCGTT AAGTTACATG CCGCAGCAGA GCGGGTATCG ACCGCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155546.1
CDS	154..2733
Translation	MDTSHHWLLVLMLLHYAKGSNSSCVLKILPDSKTKIPVELERNFTATCHLQGGSEFTSNDIKWSFSNKETTDILSKTFYHKINETSVSVTVNLSKNMGHMLMCEASRESLTYMGKCAYGIYLDAGYPPMKPKNLTCIALQRKKDISPDLNCYWEPGLRDPLLATNYTLYAQVGQDLYNSTCKQSLARSCTVNLSTYPVHMTLKVWVEVKNSLGSERSDEHEYDSLSLVKPNPPNVEVKVEEDFPTSFVVKWEYDIEDTTMRFSSAIRYCQVGSSDWKKVPENYTNVHITSYRLQSLEPYTEYVVQMRSIGEHYTIWSEWSSNVTVRTPEAKPASPPDLWRIIQHTPGKRNVTLMWKPPVKANGKMCNYSLIISQDNQAFESHVIKADQLNQRYSLEVPPEENASIEITAFNSAGGSPKTILFIPRSNDEFLGVASMRWWVHDGKLHVEWGPLKQHPPHGIHISEYLLEWVNEKDVQPQWQRVPKNVNTTILNGNLAKYKQYNISVYPIYKFYYDGRWHFQAGTPVTKPAYVQQGAPEKGPTVTDTISKKNSVTLIWEEIPLDHRYGFIQNYTIFYRTGNAEWQSVLVAANIHSYVLTDLVSERDYVVHIMASTEAGSKNGTDFNFKTMKFADGEVEMIVVLVCLGFLFLSVFTIMLCLRKREVIKKLLWPQVPDPSDSSIAHWSPDFPVKTEMPKEDVSVVEVDVFDGKSLCEEDKVVLPLKKDKYLSEEHSSGIGGSSCMSSPRHSVSDSDEGDSGQTTASTVQYSSVVASGYKGQTPNHQPPTFARSESTQPLLDCEEHPENHPGERNSYFRRGRELEPQSIDEPVFSAVQEEEASPVVDDAPALSYMPQQSGYRPQ

Target ORF information:

RefSeq Version	XM_005155489.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio interleukin 6 signal transducer (il6st), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155489.4

ORF Insert Sequence:

ATGGACACTT CACATCATTG GCTGTTGGTC TTGATGCTCT TGCATTATGC AAAAGGTTCC 
AACAGTAGCT GTGTATTAAA AATTCTTCCA GACTCCAAGA CTAAGATTCC TGTTGAGTTG 
GAAAGAAACT TCACAGCGAC ATGCCACCTC CAGGGTGGAT CTGAATTTAC CTCAAATGAC 
ATCAAATGGT CTTTTAGCAA CAAAGAAACT ACAGACATTT TATCAAAGAC ATTTTATCAC 
AAAATAAATG AAACCTCCGT CAGTGTTACA GTTAACCTCA GCAAAAACAT GGGTCATATG 
TTGATGTGTG AAGCCTCCAG AGAGTCCTTA ACATACATGG GAAAATGTGC CTACGGAATC 
TACCTTGATG CAGGATACCC TCCGATGAAA CCAAAGAATC TGACATGCAT TGCTTTGCAA 
AGGAAGAAGG ATATTTCTCC TGACCTAAAT TGTTATTGGG AACCAGGATT GAGAGACCCA 
CTGCTTGCCA CCAATTATAC TCTTTATGCC CAAGTAGGAC AAGATTTATA TAACAGCACC 
TGTAAACAGA GTCTTGCAAG AAGTTGCACC GTCAACCTGA GCACTTATCC TGTTCATATG 
ACTTTGAAAG TCTGGGTTGA AGTGAAAAAT TCACTGGGGT CAGAACGGTC AGACGAACAC 
GAGTACGATT CCTTGAGTCT TGTGAAACCA AATCCACCAA ATGTCGAAGT TAAAGTTGAA 
GAGGACTTCC CTACATCTTT TGTGGTTAAA TGGGAGTATG ACATTGAAGA CACAACTATG 
CGATTCTCCA GTGCCATTAG ATACTGCCAA GTAGGGTCAA GTGATTGGAA AAAGGTGCCT 
GAGAACTACA CCAATGTGCA CATTACGTCG TATCGACTGC AGTCCCTAGA GCCTTACACT 
GAGTATGTGG TACAGATGCG ATCCATCGGA GAGCACTACA CCATCTGGAG TGAATGGAGC 
TCCAATGTTA CAGTTCGCAC TCCAGAAGCC AAGCCAGCAA GTCCACCAGA CCTGTGGAGA 
ATCATCCAGC ACACGCCTGG GAAAAGAAAC GTCACATTAA TGTGGAAGCC TCCTGTGAAA 
GCTAATGGAA AAATGTGTAA CTATTCCCTG ATCATCAGTC AAGATAACCA GGCTTTTGAA 
AGTCACGTTA TAAAAGCTGA TCAGCTCAAC CAGAGGTATT CACTGGAAGT GCCACCAGAA 
GAAAATGCCA GTATTGAAAT AACTGCATTC AATTCAGCCG GAGGGTCTCC CAAAACTATT 
TTGTTCATTC CAAGATCAAA TGACGAGTTT CTTGGCGTGG CGAGCATGAG GTGGTGGGTT 
CATGATGGAA AGCTTCATGT GGAATGGGGT CCTTTGAAGC AGCATCCTCC TCATGGCATT 
CATATCTCAG AATATCTGCT GGAGTGGGTG AATGAAAAAG ATGTTCAGCC TCAGTGGCAG 
AGGGTTCCTA AAAATGTCAA CACCACCATA CTGAACGGTA ATTTAGCCAA ATACAAGCAG 
TACAACATAT CAGTCTATCC AATCTACAAG TTCTATTATG ATGGTCGATG GCATTTCCAA 
GCAGGAACAC CAGTAACCAA GCCAGCCTAC GTTCAGCAAG GAGCACCAGA GAAAGGCCCT 
ACTGTCACAG ATACCATTTC CAAGAAAAAT AGTGTTACAC TGATATGGGA GGAAATTCCA 
CTGGACCACC GGTATGGATT CATCCAAAAC TACACCATAT TCTACAGGAC CGGCAACGCT 
GAGTGGCAAT CTGTGCTAGT GGCCGCTAAC ATCCACTCGT ATGTATTGAC TGATCTGGTC 
AGTGAACGCG ACTATGTGGT TCACATCATG GCCTCGACGG AAGCTGGCTC CAAAAACGGC 
ACCGATTTTA ATTTCAAAAC AATGAAGTTC GCCGATGGAG AGGTGGAGAT GATTGTAGTG 
CTGGTGTGCC TCGGCTTCCT GTTCCTGAGC GTCTTCACCA TAATGCTCTG CCTTCGCAAG 
AGAGAAGTGA TTAAGAAGCT GTTATGGCCG CAAGTCCCGG ATCCGTCTGA CAGCTCTATT 
GCCCACTGGT CACCTGATTT CCCTGTAAAG ACCGAAATGC CCAAGGAAGA CGTCAGCGTG 
GTGGAAGTGG ACGTGTTTGA CGGCAAGTCT TTGTGCGAAG AAGATAAAGT TGTTCTGCCG 
CTAAAGAAGG ACAAGTACCT CTCCGAGGAG CACAGCAGCG GTATTGGAGG CTCGTCCTGC 
ATGTCTTCGC CCCGCCACAG TGTTTCCGAC AGCGATGAGG GCGACTCGGG CCAGACCACG 
GCCAGCACCG TCCAGTACTC GTCAGTGGTG GCCAGCGGAT ACAAGGGCCA AACCCCAAAC 
CATCAGCCTC CAACGTTCGC CCGCTCCGAG TCCACACAAC CGCTGCTGGA CTGCGAGGAG 
CATCCAGAGA ATCATCCCGG CGAGAGGAAC TCTTACTTTA GACGGGGCCG AGAGCTGGAG 
CCGCAGAGTA TCGATGAGCC TGTTTTCAGC GCGGTGCAGG AGGAAGAAGC ATCTCCCGTT 
GTGGATGATG CGCCGGCGTT AAGTTACATG CCGCAGCAGA GCGGGTATCG ACCGCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

il6st ( XM_005155489.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155546.1 Danio rerio interleukin 6 signal transducer (il6st), transcript variant X1, mRNA.

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CloneID	ODa15795
Clone ID Related Accession (Same CDS sequence)	XM_005155489.4 , XM_005155489.3 , NM_001365484.1
Accession Version	XM_005155489.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2580bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	interleukin-6 receptor subunit beta isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005155489.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACTT CACATCATTG GCTGTTGGTC TTGATGCTCT TGCATTATGC AAAAGGTTCC 
AACAGTAGCT GTGTATTAAA AATTCTTCCA GACTCCAAGA CTAAGATTCC TGTTGAGTTG 
GAAAGAAACT TCACAGCGAC ATGCCACCTC CAGGGTGGAT CTGAATTTAC CTCAAATGAC 
ATCAAATGGT CTTTTAGCAA CAAAGAAACT ACAGACATTT TATCAAAGAC ATTTTATCAC 
AAAATAAATG AAACCTCCGT CAGTGTTACA GTTAACCTCA GCAAAAACAT GGGTCATATG 
TTGATGTGTG AAGCCTCCAG AGAGTCCTTA ACATACATGG GAAAATGTGC CTACGGAATC 
TACCTTGATG CAGGATACCC TCCGATGAAA CCAAAGAATC TGACATGCAT TGCTTTGCAA 
AGGAAGAAGG ATATTTCTCC TGACCTAAAT TGTTATTGGG AACCAGGATT GAGAGACCCA 
CTGCTTGCCA CCAATTATAC TCTTTATGCC CAAGTAGGAC AAGATTTATA TAACAGCACC 
TGTAAACAGA GTCTTGCAAG AAGTTGCACC GTCAACCTGA GCACTTATCC TGTTCATATG 
ACTTTGAAAG TCTGGGTTGA AGTGAAAAAT TCACTGGGGT CAGAACGGTC AGACGAACAC 
GAGTACGATT CCTTGAGTCT TGTGAAACCA AATCCACCAA ATGTCGAAGT TAAAGTTGAA 
GAGGACTTCC CTACATCTTT TGTGGTTAAA TGGGAGTATG ACATTGAAGA CACAACTATG 
CGATTCTCCA GTGCCATTAG ATACTGCCAA GTAGGGTCAA GTGATTGGAA AAAGGTGCCT 
GAGAACTACA CCAATGTGCA CATTACGTCG TATCGACTGC AGTCCCTAGA GCCTTACACT 
GAGTATGTGG TACAGATGCG ATCCATCGGA GAGCACTACA CCATCTGGAG TGAATGGAGC 
TCCAATGTTA CAGTTCGCAC TCCAGAAGCC AAGCCAGCAA GTCCACCAGA CCTGTGGAGA 
ATCATCCAGC ACACGCCTGG GAAAAGAAAC GTCACATTAA TGTGGAAGCC TCCTGTGAAA 
GCTAATGGAA AAATGTGTAA CTATTCCCTG ATCATCAGTC AAGATAACCA GGCTTTTGAA 
AGTCACGTTA TAAAAGCTGA TCAGCTCAAC CAGAGGTATT CACTGGAAGT GCCACCAGAA 
GAAAATGCCA GTATTGAAAT AACTGCATTC AATTCAGCCG GAGGGTCTCC CAAAACTATT 
TTGTTCATTC CAAGATCAAA TGACGAGTTT CTTGGCGTGG CGAGCATGAG GTGGTGGGTT 
CATGATGGAA AGCTTCATGT GGAATGGGGT CCTTTGAAGC AGCATCCTCC TCATGGCATT 
CATATCTCAG AATATCTGCT GGAGTGGGTG AATGAAAAAG ATGTTCAGCC TCAGTGGCAG 
AGGGTTCCTA AAAATGTCAA CACCACCATA CTGAACGGTA ATTTAGCCAA ATACAAGCAG 
TACAACATAT CAGTCTATCC AATCTACAAG TTCTATTATG ATGGTCGATG GCATTTCCAA 
GCAGGAACAC CAGTAACCAA GCCAGCCTAC GTTCAGCAAG GAGCACCAGA GAAAGGCCCT 
ACTGTCACAG ATACCATTTC CAAGAAAAAT AGTGTTACAC TGATATGGGA GGAAATTCCA 
CTGGACCACC GGTATGGATT CATCCAAAAC TACACCATAT TCTACAGGAC CGGCAACGCT 
GAGTGGCAAT CTGTGCTAGT GGCCGCTAAC ATCCACTCGT ATGTATTGAC TGATCTGGTC 
AGTGAACGCG ACTATGTGGT TCACATCATG GCCTCGACGG AAGCTGGCTC CAAAAACGGC 
ACCGATTTTA ATTTCAAAAC AATGAAGTTC GCCGATGGAG AGGTGGAGAT GATTGTAGTG 
CTGGTGTGCC TCGGCTTCCT GTTCCTGAGC GTCTTCACCA TAATGCTCTG CCTTCGCAAG 
AGAGAAGTGA TTAAGAAGCT GTTATGGCCG CAAGTCCCGG ATCCGTCTGA CAGCTCTATT 
GCCCACTGGT CACCTGATTT CCCTGTAAAG ACCGAAATGC CCAAGGAAGA CGTCAGCGTG 
GTGGAAGTGG ACGTGTTTGA CGGCAAGTCT TTGTGCGAAG AAGATAAAGT TGTTCTGCCG 
CTAAAGAAGG ACAAGTACCT CTCCGAGGAG CACAGCAGCG GTATTGGAGG CTCGTCCTGC 
ATGTCTTCGC CCCGCCACAG TGTTTCCGAC AGCGATGAGG GCGACTCGGG CCAGACCACG 
GCCAGCACCG TCCAGTACTC GTCAGTGGTG GCCAGCGGAT ACAAGGGCCA AACCCCAAAC 
CATCAGCCTC CAACGTTCGC CCGCTCCGAG TCCACACAAC CGCTGCTGGA CTGCGAGGAG 
CATCCAGAGA ATCATCCCGG CGAGAGGAAC TCTTACTTTA GACGGGGCCG AGAGCTGGAG 
CCGCAGAGTA TCGATGAGCC TGTTTTCAGC GCGGTGCAGG AGGAAGAAGC ATCTCCCGTT 
GTGGATGATG CGCCGGCGTT AAGTTACATG CCGCAGCAGA GCGGGTATCG ACCGCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155546.1
CDS	146..2725
Translation	MDTSHHWLLVLMLLHYAKGSNSSCVLKILPDSKTKIPVELERNFTATCHLQGGSEFTSNDIKWSFSNKETTDILSKTFYHKINETSVSVTVNLSKNMGHMLMCEASRESLTYMGKCAYGIYLDAGYPPMKPKNLTCIALQRKKDISPDLNCYWEPGLRDPLLATNYTLYAQVGQDLYNSTCKQSLARSCTVNLSTYPVHMTLKVWVEVKNSLGSERSDEHEYDSLSLVKPNPPNVEVKVEEDFPTSFVVKWEYDIEDTTMRFSSAIRYCQVGSSDWKKVPENYTNVHITSYRLQSLEPYTEYVVQMRSIGEHYTIWSEWSSNVTVRTPEAKPASPPDLWRIIQHTPGKRNVTLMWKPPVKANGKMCNYSLIISQDNQAFESHVIKADQLNQRYSLEVPPEENASIEITAFNSAGGSPKTILFIPRSNDEFLGVASMRWWVHDGKLHVEWGPLKQHPPHGIHISEYLLEWVNEKDVQPQWQRVPKNVNTTILNGNLAKYKQYNISVYPIYKFYYDGRWHFQAGTPVTKPAYVQQGAPEKGPTVTDTISKKNSVTLIWEEIPLDHRYGFIQNYTIFYRTGNAEWQSVLVAANIHSYVLTDLVSERDYVVHIMASTEAGSKNGTDFNFKTMKFADGEVEMIVVLVCLGFLFLSVFTIMLCLRKREVIKKLLWPQVPDPSDSSIAHWSPDFPVKTEMPKEDVSVVEVDVFDGKSLCEEDKVVLPLKKDKYLSEEHSSGIGGSSCMSSPRHSVSDSDEGDSGQTTASTVQYSSVVASGYKGQTPNHQPPTFARSESTQPLLDCEEHPENHPGERNSYFRRGRELEPQSIDEPVFSAVQEEEASPVVDDAPALSYMPQQSGYRPQ

Target ORF information:

RefSeq Version	XM_005155489.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio interleukin 6 signal transducer (il6st), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155489.3

ORF Insert Sequence:

ATGGACACTT CACATCATTG GCTGTTGGTC TTGATGCTCT TGCATTATGC AAAAGGTTCC 
AACAGTAGCT GTGTATTAAA AATTCTTCCA GACTCCAAGA CTAAGATTCC TGTTGAGTTG 
GAAAGAAACT TCACAGCGAC ATGCCACCTC CAGGGTGGAT CTGAATTTAC CTCAAATGAC 
ATCAAATGGT CTTTTAGCAA CAAAGAAACT ACAGACATTT TATCAAAGAC ATTTTATCAC 
AAAATAAATG AAACCTCCGT CAGTGTTACA GTTAACCTCA GCAAAAACAT GGGTCATATG 
TTGATGTGTG AAGCCTCCAG AGAGTCCTTA ACATACATGG GAAAATGTGC CTACGGAATC 
TACCTTGATG CAGGATACCC TCCGATGAAA CCAAAGAATC TGACATGCAT TGCTTTGCAA 
AGGAAGAAGG ATATTTCTCC TGACCTAAAT TGTTATTGGG AACCAGGATT GAGAGACCCA 
CTGCTTGCCA CCAATTATAC TCTTTATGCC CAAGTAGGAC AAGATTTATA TAACAGCACC 
TGTAAACAGA GTCTTGCAAG AAGTTGCACC GTCAACCTGA GCACTTATCC TGTTCATATG 
ACTTTGAAAG TCTGGGTTGA AGTGAAAAAT TCACTGGGGT CAGAACGGTC AGACGAACAC 
GAGTACGATT CCTTGAGTCT TGTGAAACCA AATCCACCAA ATGTCGAAGT TAAAGTTGAA 
GAGGACTTCC CTACATCTTT TGTGGTTAAA TGGGAGTATG ACATTGAAGA CACAACTATG 
CGATTCTCCA GTGCCATTAG ATACTGCCAA GTAGGGTCAA GTGATTGGAA AAAGGTGCCT 
GAGAACTACA CCAATGTGCA CATTACGTCG TATCGACTGC AGTCCCTAGA GCCTTACACT 
GAGTATGTGG TACAGATGCG ATCCATCGGA GAGCACTACA CCATCTGGAG TGAATGGAGC 
TCCAATGTTA CAGTTCGCAC TCCAGAAGCC AAGCCAGCAA GTCCACCAGA CCTGTGGAGA 
ATCATCCAGC ACACGCCTGG GAAAAGAAAC GTCACATTAA TGTGGAAGCC TCCTGTGAAA 
GCTAATGGAA AAATGTGTAA CTATTCCCTG ATCATCAGTC AAGATAACCA GGCTTTTGAA 
AGTCACGTTA TAAAAGCTGA TCAGCTCAAC CAGAGGTATT CACTGGAAGT GCCACCAGAA 
GAAAATGCCA GTATTGAAAT AACTGCATTC AATTCAGCCG GAGGGTCTCC CAAAACTATT 
TTGTTCATTC CAAGATCAAA TGACGAGTTT CTTGGCGTGG CGAGCATGAG GTGGTGGGTT 
CATGATGGAA AGCTTCATGT GGAATGGGGT CCTTTGAAGC AGCATCCTCC TCATGGCATT 
CATATCTCAG AATATCTGCT GGAGTGGGTG AATGAAAAAG ATGTTCAGCC TCAGTGGCAG 
AGGGTTCCTA AAAATGTCAA CACCACCATA CTGAACGGTA ATTTAGCCAA ATACAAGCAG 
TACAACATAT CAGTCTATCC AATCTACAAG TTCTATTATG ATGGTCGATG GCATTTCCAA 
GCAGGAACAC CAGTAACCAA GCCAGCCTAC GTTCAGCAAG GAGCACCAGA GAAAGGCCCT 
ACTGTCACAG ATACCATTTC CAAGAAAAAT AGTGTTACAC TGATATGGGA GGAAATTCCA 
CTGGACCACC GGTATGGATT CATCCAAAAC TACACCATAT TCTACAGGAC CGGCAACGCT 
GAGTGGCAAT CTGTGCTAGT GGCCGCTAAC ATCCACTCGT ATGTATTGAC TGATCTGGTC 
AGTGAACGCG ACTATGTGGT TCACATCATG GCCTCGACGG AAGCTGGCTC CAAAAACGGC 
ACCGATTTTA ATTTCAAAAC AATGAAGTTC GCCGATGGAG AGGTGGAGAT GATTGTAGTG 
CTGGTGTGCC TCGGCTTCCT GTTCCTGAGC GTCTTCACCA TAATGCTCTG CCTTCGCAAG 
AGAGAAGTGA TTAAGAAGCT GTTATGGCCG CAAGTCCCGG ATCCGTCTGA CAGCTCTATT 
GCCCACTGGT CACCTGATTT CCCTGTAAAG ACCGAAATGC CCAAGGAAGA CGTCAGCGTG 
GTGGAAGTGG ACGTGTTTGA CGGCAAGTCT TTGTGCGAAG AAGATAAAGT TGTTCTGCCG 
CTAAAGAAGG ACAAGTACCT CTCCGAGGAG CACAGCAGCG GTATTGGAGG CTCGTCCTGC 
ATGTCTTCGC CCCGCCACAG TGTTTCCGAC AGCGATGAGG GCGACTCGGG CCAGACCACG 
GCCAGCACCG TCCAGTACTC GTCAGTGGTG GCCAGCGGAT ACAAGGGCCA AACCCCAAAC 
CATCAGCCTC CAACGTTCGC CCGCTCCGAG TCCACACAAC CGCTGCTGGA CTGCGAGGAG 
CATCCAGAGA ATCATCCCGG CGAGAGGAAC TCTTACTTTA GACGGGGCCG AGAGCTGGAG 
CCGCAGAGTA TCGATGAGCC TGTTTTCAGC GCGGTGCAGG AGGAAGAAGC ATCTCCCGTT 
GTGGATGATG CGCCGGCGTT AAGTTACATG CCGCAGCAGA GCGGGTATCG ACCGCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Inflammatory response and blood hypercoagulable state induced by low level co-exposure with silica nanoparticles and benzo[a]pyrene in zebrafish (Danio rerio) embryos.
Chemosphere151152-62(2016 May)
Duan J,Yu Y,Li Y,Wang Y,Sun Z

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

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il6st cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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