arhgap23a gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_005164176.4	XP_005164233.1	rho GTPase-activating protein 23 isoform X6
XM_005164175.3	XP_005164232.1	rho GTPase-activating protein 23 isoform X5
XM_005164176.3	XP_005164233.1	rho GTPase-activating protein 23 isoform X6
XM_005164174.3	XP_005164231.1	rho GTPase-activating protein 23 isoform X2
XM_005164174.4	XP_005164231.1	rho GTPase-activating protein 23 isoform X2
XM_009299680.3	XP_009297955.1	rho GTPase-activating protein 23 isoform X3
XM_005164175.4	XP_005164232.1	rho GTPase-activating protein 23 isoform X5
XM_009299680.2	XP_009297955.1	rho GTPase-activating protein 23 isoform X3
XM_003198148.5	XP_003198196.2	rho GTPase-activating protein 23 isoform X1
XM_003198148.4	XP_003198196.2	rho GTPase-activating protein 23 isoform X1
XM_009299681.2	XP_009297956.1	rho GTPase-activating protein 23 isoform X4
XM_021474705.1	XP_021330380.1	rho GTPase-activating protein 23 isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa08640	XM_003198148.5 Latest version!	Danio rerio Rho GTPase activating protein 23a (arhgap23a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa08641	XM_005164174.4 Latest version!	Danio rerio Rho GTPase activating protein 23a (arhgap23a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa08642	XM_005164175.4 Latest version!	Danio rerio Rho GTPase activating protein 23a (arhgap23a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa08643	XM_005164176.4 Latest version!	Danio rerio Rho GTPase activating protein 23a (arhgap23a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa48127	XM_009299680.3 Latest version!	Danio rerio Rho GTPase activating protein 23a (arhgap23a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa48128	XM_021474705.1 Latest version!	Danio rerio Rho GTPase activating protein 23a (arhgap23a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa48128	XM_009299681.2 Latest version!	Danio rerio Rho GTPase activating protein 23a (arhgap23a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
ODa08643	XM_005164176.3	Danio rerio Rho GTPase activating protein 23a (arhgap23a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa08641	XM_005164174.3	Danio rerio Rho GTPase activating protein 23a (arhgap23a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa08642	XM_005164175.3	Danio rerio Rho GTPase activating protein 23a (arhgap23a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa48127	XM_009299680.2	Danio rerio Rho GTPase activating protein 23a (arhgap23a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa08640	XM_003198148.4	Danio rerio Rho GTPase activating protein 23a (arhgap23a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

arhgap23a ( XM_003198148.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_003198196.2 Danio rerio Rho GTPase activating protein 23a (arhgap23a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa08640
Clone ID Related Accession (Same CDS sequence)	XM_003198148.5 , XM_003198148.4
Accession Version	XM_003198148.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	rho GTPase-activating protein 23 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_003198148.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCAG CAGGTCAAGT GCCACTGGCG ATGCCCGTTG TGTGCCCGCA TGTAGGGGGT 
AAGGAGTGGT GCTTTCAGGA TGCAGTCGGG GTGGATTGCA GCTCTCCAGA GCCTCGCTGT 
ATCTGGGTGG CGGTTTTACA CGGGGATACA CAGCAAATTA ACATTTCCAA CCTCCAAAAT 
GACTCTTCTC CAAACATAAA TGGCATCAGT GCTGGGAAAG CACTGTCAAG ACCCGCACAG 
ACCTCCAGGG CAAAAGGGCG GAGAGATGGG CTCTCTTCAT CCAGTGAGAA CCCTCGGCCG 
GTGGTCCGGA GCGGGACAGA TGGGCTGGGC GTGTCCTGGC AGGGTCCACG CACGCTGGTG 
CTCCAAAAGA ACTCTCAGGG CTTCGGCTTT ACTCTGCGTC ACTTCATCGT TTACCCGCCA 
GAGTCCGCTC TGCACACCAC TCTCAAGGAT GAAGAGAACG GGAATGGAAA GGGGCTTCAA 
CGAAGTCGTC TGGAGCCCAT GGACACAATT TTTGTGAAGA ATGTGAGGGA AAAAGGCCCC 
GCTCACCTGG CTGGCCTGTG TACAGGAGAC AGGCTGGTTA AAGTGAATGG GGAGAGCGTG 
CTGGGTAAGA CGTACTCGCA GGTCATTGCA CTGATTCAGA ACAGTGAGAG TGTACTGGAG 
CTCTCCATTA TGCCAAAGGA TGAGGACGTG CTTCAGCTGG CATATTCCCA TGATGCCTAC 
CTAAGGGGGA ACAATCCATA TACAGGAAAG GCCCAAAATC TTCCTCCACC ACCCCCTGTT 
CTCTACCCTC CTCGTACAAA ATCACAGTCT CCTGCAGGGT TCAGCCCCTG CCCTCCTCCA 
GCTTCTTCAC ACATGGGGCA AAACCAGCTG GACAACTGGA GCCGCTGGCC CGGCTCTTCT 
GCTGGTCCCT CTCCACCCCT TGACAACCGG ACTGTGGCCT CCAGCAATGT TGTCTGGGCA 
GCCGAGGGCA GGAATTGTGA GCCGGGTGGT GTTGGTCACA GTAGCCCTGC ACACCGAACA 
GAGGAGATCC AGTATGGAAT GACTGACAGA GGACAGCAGC CAACTGGACA AATGAGAGGA 
CGCTCATACT CATCTTCTTC CTCCTCAGGA GGAATGATGA GCCCTTTGCA TCATGGTCAT 
CTTGCCAATC ACAACATGGG TACTGCCATG GGGCACACTG GAGGTCGTAA GGGAAGCCCA 
CCTTGGGCCA GCCCTTCTCA GCCAACATCA ACACCTTCAT CCAGCCGCAA CGAACGAACC 
CAACAAGCCC TTTCCAACTG GTACTACAGC CAGGTCCCAG GCCGTGAGCG AGAACGAGAA 
CGAGAGCGGG AGCGATCTAG TTCTCGCTCA ATGCACCCAC GCCACAGGAG TTTCTCACAA 
GACCGTCTGG GAGAGCTTGG GAAGGCCAGG CGTTCTCAAC AAGCAGGTTT TCCCCATAGT 
GCATCGCAAG ACACGTTGCT GCTGCTTCAG CAGAGTGCTG CAGCATATGC TGATCCTTAC 
TGGAACCATG GAGACTGGCG GGGTGAGCAT ATGTATGGTC AGCACCCATC TGTGGTGGCC 
TATGGGCGAA AGTCCTCCGA AAATCTTCTA GCAAGCCGGT ACGGACACTC GGGACGCTCA 
CTGGAAAATC TCGACAGAGC TGTGGGGATA CTCTCACCAA GGGTTGAAAG ATCTGCTTGG 
GTTCAACAAC CTCCAAATAG GACTGAAGTC TATGCAAGAC ATGCTGGGCA TTTCGGCCAA 
CATATAGAGT CACCCCACCA AATGCACCAC ACACCCCAAC ACCACCAGAA TCTCACACAT 
GCAACACACC AACACTCATC TCAAATCCCA CAACCTCAGC AGGCAGCTCC AATGGCAAGA 
CGTTTGCCTT CTTGCCAGAG TCTAGATGAG GAGCCGATTG GCTATCGTAG CTACAGTCCC 
TCATTCAATA GGAAGACTGG CCGTATCTTG CAGCAGGCCC AGTCATTTAG AGATCCCTCT 
TACACAGGTC CACACCTTTG GACTCCAACA TCCAAAACCA GTCCCCCAGA GGGGCATCCA 
CCCTCAGCCA CTGCTTCCCC AGCCCCACCA ACCACTTCCT CTTCAACTAC GATTTCCACA 
CCATCTGAAG GACAAGACAG AACTTACCGA CCCACTAATC ATGACAGAGG GGCAGGGGAA 
GCGACAAGTG AAGCCCAGAC ACAGGAAGTG GTTCTGAGGC AGAAACCACC TACCGGCCGA 
CGAGGTGCAA ACGCCCTTCG ACACCCACAT TATGCAATAG CACTTGACTC CAGTGACCCT 
CTACTCTTTA CCACTGACCC TACGGAGCCC ACTGCCAAGG CAGAAAGTGC TATTTCTCAT 
CGCCGCACAG ATGTTAACCT TCCTCCACTA TCTATTGAGG ATGACTCCCT TGCATCAATT 
CCATTCATCG ATGAGCCGAC CAGTCCAAGT GCAGATCTGC GGGCACGTCA TGTCCCCGCC 
TCCTCCGTCG TGTCCAGTGG CGGTATAAAC TCCGCCCCTG CTGTGATCAC AAGCCCCGCC 
TCCCCAACCT TCACCTTTCC CCTCAACCGC CTTTTCTCCC ACAACTGCAG TGATCCTAAA 
TCTAATCGTC GCTCCTCGTA CCTGTTGGCC ATCACCACTG AACGCTCCAA GTCATGTGAT 
GAGGGGCTTA ACGCATATAG AGAAGAGGGC CGGGTCTTCT CGAGGCTTCC AAAAAGAGTC 
AAGAGCTTCT TTACTGAAGG GTCTCTGGAC AGTCTGGGTG CTGCTGAAGA GGCTCGCTCT 
AAACGACACT CTACCTCTGA GCTGGGCAAC ATCAGCTACA GTGACGTGAA GAGAGAAGGC 
TGGCTCCACT TCAAACAGAT CCACACGGAG AAGGGCAAGA AGGTTGGCAG TGCAATCTGG 
CCATGGAGAC GGGTGTTCTC TGTGCTTAGG ACCCATTCTC TGTTTCTTTA TAAAGACAAG 
CGAGAGGCAG TGCTTAAAGG GGCGCAATTA GGGGGCGGGG CTGAAGACGA GCAGCCAATC 
AGCATCCGGG GCTGCCTTGT GGATATAGCA TACAGTGAAA CGAAGCGCAA GCACGCGCTG 
AGATTGACCA CGCAGGACTT CTGCGAGTAC CTGCTGCAGG CGGAGGACAG AGAGGACATG 
CTGGAGTGGA TCCGAGTCAT CAGGGACAAC AGCAAGACCG ACAACGAGGA ATTGGGTTTC 
TCACGACAAG CTCTCATCAA TAAAAAGCTT AACGACTACA GGAAGCAAAG TCCAACGGGT 
AACAAACCAG ACTCCTCACC CAGAGTGCAT CGAATGACCT TCCTCCTCAG CAAGCCAGAC 
AACAGCGCAC CTCCACGCTC CCCAAAACAC GAGGCCAAAG ATGAGAGCAG CCCCCCAAAA 
TCTCCCTGGG GTATAAACAT AATGAAGAAG AGCAAGAAGG CCGGTCCTAA AGCTTTTGGC 
GTCCGTCTAG AGGACTGCCA GCCTGCAGTA AACAACAAGT TCATTCCTCA GATTGTGGAG 
ATCTGCTGTG GGCTGGTGGA GGACATGGGT CTGGAGTATA CAGGGATCTA CAGAGTGCCA 
GGAAACAATG CTGTAGTCTC CAGTCTGCAG GAGCAGCTCA ACAAAGGAGT AGACATTAAC 
ATCACTGAGG AGAAATGGCA GGACCTGAAT GTGGTGAGCA GCCTGCTGAA GTCCTTCTTT 
CGTAAACTCC CTGAACCCCT CTTCACTGAT GATAAATATA ATGACTTTAT CGACGCCAAT 
CGGATGGAGA ACGCCAGCGA TAGGCTGAGG ACAATGAAGA AATTGATTCG GGACCTACCA 
GATCATTACT TTCACACTCT GAAATTTTTA GTTGGACACC TAAAGACGGT GGCTGACCAC 
TCTGAGAAAA ACAAAATGGA GCCGCGTAAT TTGGCTCTGG TGTTTGGCCC CACTCTTGTT 
CGAACTTCAG AGGACAACAT GACAGACATG GTCACCCACA TGCCAGACCG CTACAAGATT 
GTGGAGACAC TCATTCAGCA TTACACCTGG TTTTTCTGCG AAGAACAGGA TAAGGATGAA 
AAGACCCCAG TGGACACAGA GGATGTCCAG CCTGCCCCAA ACATTGATCA TCTGCTCTCC 
AACATTGGCC GCACAGGCAT GCTGGGTGAG GCTTCAGACT CAACCAACAG TGATTCTGCA 
AAATCTAAGG GGTCATGGGG ATCAAAACGT GACTTTACTG CCAAGGATAT CCTTACATTA 
TCAATTATGT CGGCTGTGAC CCGCAAACGG CGGAAGCGGC ACAACGGCCG TCTCCTCGGC 
AGCAGCACAG ACGACGATTC TGAGCATGAA CCCATTAAAT CCTGCATCTC CGAGAAAGAC 
GATCTGCAAG AGATGGGGCA GGTTGCATCC TTCTGTGCCC CACGAGCAGA GGGAGAGGAG 
GATGACGATG AAGAGGAGGA AGATGATGAA GAGGAGGAGG AGGGGGCTGA GAGACAGAAG 
GCACAAGAGC AGAAGGAAAC AGTAGTCCCC AGCATTTCCT CAACAGAGGA AGTGACATCA 
ACAGTGCTGC TGAGCGAAGA GGAAGAGCAG AAGTCTGAAG TGAAAGGAGG ACGCAGCTGG 
AGATTAGATG ATGCTCGGTC TATTGTGTCA GGCTACTCTA CTCTGTCCAC TTTGGGGCGG 
AGCTTAGCTT CAGAGGGGCG GGGCGAGGAT GCAGATGATG AGCATAGTGA ACTAGTGAGT 
GAAACTGATA ATGAAAGCGG GTTTGCCTCA CGCTCCCTCA CCCAAGAAAG GCCAGAAAAA 
CCAAACCGTT CCAACCAGAA CTCTCATGCG TCTCCTGAAC CAACCGCACC ACAGAGTTTC 
CTCTATGCGC ACTGCAAATC ACCTACACCC TCTGGCTCCG CCACTGCTCC ATCTCCCCCT 
TCACAACACA TCACAACCAA CCCAGAGGAC AGCGCAGCAC GCTCGTCTAC TCCCTCAACC 
TCCTCTTACT CCTCATCCGC CTCACAACGG CTCCACAACC GCTCTTTCAA CTCCCACCGC 
CTCATACAGT GCGACACCCT TGCACGCCGT CGGACAAAGG CGGACAAAGC CAAAGCTCCC 
TCAGTGGACC TTTCCGAACT CTCGAGCTCC TCCGCCGCAG AGGAGGACCG CCAGTCAGGT 
GCCAATCAAA TCCCTGACTC CTCTCCGTCT CCTTCTTCAA AAGCCAAAGG GTTTAGCATA 
AGAAACGAGT GTACGCCAAT ATCCATGGTT GGTCCCGGAA GTAAGGGGTG TGTGTTGTCC 
CCCGGGCAAG GATCCCTGGC GGATCAGGTA CGTGCTCGTC TAATGGGCTC GGCGGATGAC 
CTGTGCAGCG TTGGCCTGAG AAAGCAGCTC TCGCCGGAGA CGCGCCGGAA GAGACGCGCT 
TGGAGGAGAC ACACAGTGGT CGTGTCCCCT ACGGATTGTT CAGACAAAAA GCCAACGGTC 
CAAAGCACCA AACCTCTAGC TTCACCCTCC CCTGTTGAAA AGCAGGATGT CAGAGAGTCC 
CACCCAGAGT GCTCAGAAAC TGAAGCCCCT ACTGCACGTC GGGCCGTGCC TACCTCGCGC 
TTTCGTGATT TCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003198196.2
CDS	988..6525
Translation	MWSAGQVPLAMPVVCPHVGGKEWCFQDAVGVDCSSPEPRCIWVAVLHGDTQQINISNLQNDSSPNINGISAGKALSRPAQTSRAKGRRDGLSSSSENPRPVVRSGTDGLGVSWQGPRTLVLQKNSQGFGFTLRHFIVYPPESALHTTLKDEENGNGKGLQRSRLEPMDTIFVKNVREKGPAHLAGLCTGDRLVKVNGESVLGKTYSQVIALIQNSESVLELSIMPKDEDVLQLAYSHDAYLRGNNPYTGKAQNLPPPPPVLYPPRTKSQSPAGFSPCPPPASSHMGQNQLDNWSRWPGSSAGPSPPLDNRTVASSNVVWAAEGRNCEPGGVGHSSPAHRTEEIQYGMTDRGQQPTGQMRGRSYSSSSSSGGMMSPLHHGHLANHNMGTAMGHTGGRKGSPPWASPSQPTSTPSSSRNERTQQALSNWYYSQVPGRERERERERERSSSRSMHPRHRSFSQDRLGELGKARRSQQAGFPHSASQDTLLLLQQSAAAYADPYWNHGDWRGEHMYGQHPSVVAYGRKSSENLLASRYGHSGRSLENLDRAVGILSPRVERSAWVQQPPNRTEVYARHAGHFGQHIESPHQMHHTPQHHQNLTHATHQHSSQIPQPQQAAPMARRLPSCQSLDEEPIGYRSYSPSFNRKTGRILQQAQSFRDPSYTGPHLWTPTSKTSPPEGHPPSATASPAPPTTSSSTTISTPSEGQDRTYRPTNHDRGAGEATSEAQTQEVVLRQKPPTGRRGANALRHPHYAIALDSSDPLLFTTDPTEPTAKAESAISHRRTDVNLPPLSIEDDSLASIPFIDEPTSPSADLRARHVPASSVVSSGGINSAPAVITSPASPTFTFPLNRLFSHNCSDPKSNRRSSYLLAITTERSKSCDEGLNAYREEGRVFSRLPKRVKSFFTEGSLDSLGAAEEARSKRHSTSELGNISYSDVKREGWLHFKQIHTEKGKKVGSAIWPWRRVFSVLRTHSLFLYKDKREAVLKGAQLGGGAEDEQPISIRGCLVDIAYSETKRKHALRLTTQDFCEYLLQAEDREDMLEWIRVIRDNSKTDNEELGFSRQALINKKLNDYRKQSPTGNKPDSSPRVHRMTFLLSKPDNSAPPRSPKHEAKDESSPPKSPWGINIMKKSKKAGPKAFGVRLEDCQPAVNNKFIPQIVEICCGLVEDMGLEYTGIYRVPGNNAVVSSLQEQLNKGVDINITEEKWQDLNVVSSLLKSFFRKLPEPLFTDDKYNDFIDANRMENASDRLRTMKKLIRDLPDHYFHTLKFLVGHLKTVADHSEKNKMEPRNLALVFGPTLVRTSEDNMTDMVTHMPDRYKIVETLIQHYTWFFCEEQDKDEKTPVDTEDVQPAPNIDHLLSNIGRTGMLGEASDSTNSDSAKSKGSWGSKRDFTAKDILTLSIMSAVTRKRRKRHNGRLLGSSTDDDSEHEPIKSCISEKDDLQEMGQVASFCAPRAEGEEDDDEEEEDDEEEEEGAERQKAQEQKETVVPSISSTEEVTSTVLLSEEEEQKSEVKGGRSWRLDDARSIVSGYSTLSTLGRSLASEGRGEDADDEHSELVSETDNESGFASRSLTQERPEKPNRSNQNSHASPEPTAPQSFLYAHCKSPTPSGSATAPSPPSQHITTNPEDSAARSSTPSTSSYSSSASQRLHNRSFNSHRLIQCDTLARRRTKADKAKAPSVDLSELSSSSAAEEDRQSGANQIPDSSPSPSSKAKGFSIRNECTPISMVGPGSKGCVLSPGQGSLADQVRARLMGSADDLCSVGLRKQLSPETRRKRRAWRRHTVVVSPTDCSDKKPTVQSTKPLASPSPVEKQDVRESHPECSETEAPTARRAVPTSRFRDFL

Target ORF information:

RefSeq Version	XM_003198148.5
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Rho GTPase activating protein 23a (arhgap23a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003198148.5

ORF Insert Sequence:

ATGTGGTCAG CAGGTCAAGT GCCACTGGCG ATGCCCGTTG TGTGCCCGCA TGTAGGGGGT 
AAGGAGTGGT GCTTTCAGGA TGCAGTCGGG GTGGATTGCA GCTCTCCAGA GCCTCGCTGT 
ATCTGGGTGG CGGTTTTACA CGGGGATACA CAGCAAATTA ACATTTCCAA CCTCCAAAAT 
GACTCTTCTC CAAACATAAA TGGCATCAGT GCTGGGAAAG CACTGTCAAG ACCCGCACAG 
ACCTCCAGGG CAAAAGGGCG GAGAGATGGG CTCTCTTCAT CCAGTGAGAA CCCTCGGCCG 
GTGGTCCGGA GCGGGACAGA TGGGCTGGGC GTGTCCTGGC AGGGTCCACG CACGCTGGTG 
CTCCAAAAGA ACTCTCAGGG CTTCGGCTTT ACTCTGCGTC ACTTCATCGT TTACCCGCCA 
GAGTCCGCTC TGCACACCAC TCTCAAGGAT GAAGAGAACG GGAATGGAAA GGGGCTTCAA 
CGAAGTCGTC TGGAGCCCAT GGACACAATT TTTGTGAAGA ATGTGAGGGA AAAAGGCCCC 
GCTCACCTGG CTGGCCTGTG TACAGGAGAC AGGCTGGTTA AAGTGAATGG GGAGAGCGTG 
CTGGGTAAGA CGTACTCGCA GGTCATTGCA CTGATTCAGA ACAGTGAGAG TGTACTGGAG 
CTCTCCATTA TGCCAAAGGA TGAGGACGTG CTTCAGCTGG CATATTCCCA TGATGCCTAC 
CTAAGGGGGA ACAATCCATA TACAGGAAAG GCCCAAAATC TTCCTCCACC ACCCCCTGTT 
CTCTACCCTC CTCGTACAAA ATCACAGTCT CCTGCAGGGT TCAGCCCCTG CCCTCCTCCA 
GCTTCTTCAC ACATGGGGCA AAACCAGCTG GACAACTGGA GCCGCTGGCC CGGCTCTTCT 
GCTGGTCCCT CTCCACCCCT TGACAACCGG ACTGTGGCCT CCAGCAATGT TGTCTGGGCA 
GCCGAGGGCA GGAATTGTGA GCCGGGTGGT GTTGGTCACA GTAGCCCTGC ACACCGAACA 
GAGGAGATCC AGTATGGAAT GACTGACAGA GGACAGCAGC CAACTGGACA AATGAGAGGA 
CGCTCATACT CATCTTCTTC CTCCTCAGGA GGAATGATGA GCCCTTTGCA TCATGGTCAT 
CTTGCCAATC ACAACATGGG TACTGCCATG GGGCACACTG GAGGTCGTAA GGGAAGCCCA 
CCTTGGGCCA GCCCTTCTCA GCCAACATCA ACACCTTCAT CCAGCCGCAA CGAACGAACC 
CAACAAGCCC TTTCCAACTG GTACTACAGC CAGGTCCCAG GCCGTGAGCG AGAACGAGAA 
CGAGAGCGGG AGCGATCTAG TTCTCGCTCA ATGCACCCAC GCCACAGGAG TTTCTCACAA 
GACCGTCTGG GAGAGCTTGG GAAGGCCAGG CGTTCTCAAC AAGCAGGTTT TCCCCATAGT 
GCATCGCAAG ACACGTTGCT GCTGCTTCAG CAGAGTGCTG CAGCATATGC TGATCCTTAC 
TGGAACCATG GAGACTGGCG GGGTGAGCAT ATGTATGGTC AGCACCCATC TGTGGTGGCC 
TATGGGCGAA AGTCCTCCGA AAATCTTCTA GCAAGCCGGT ACGGACACTC GGGACGCTCA 
CTGGAAAATC TCGACAGAGC TGTGGGGATA CTCTCACCAA GGGTTGAAAG ATCTGCTTGG 
GTTCAACAAC CTCCAAATAG GACTGAAGTC TATGCAAGAC ATGCTGGGCA TTTCGGCCAA 
CATATAGAGT CACCCCACCA AATGCACCAC ACACCCCAAC ACCACCAGAA TCTCACACAT 
GCAACACACC AACACTCATC TCAAATCCCA CAACCTCAGC AGGCAGCTCC AATGGCAAGA 
CGTTTGCCTT CTTGCCAGAG TCTAGATGAG GAGCCGATTG GCTATCGTAG CTACAGTCCC 
TCATTCAATA GGAAGACTGG CCGTATCTTG CAGCAGGCCC AGTCATTTAG AGATCCCTCT 
TACACAGGTC CACACCTTTG GACTCCAACA TCCAAAACCA GTCCCCCAGA GGGGCATCCA 
CCCTCAGCCA CTGCTTCCCC AGCCCCACCA ACCACTTCCT CTTCAACTAC GATTTCCACA 
CCATCTGAAG GACAAGACAG AACTTACCGA CCCACTAATC ATGACAGAGG GGCAGGGGAA 
GCGACAAGTG AAGCCCAGAC ACAGGAAGTG GTTCTGAGGC AGAAACCACC TACCGGCCGA 
CGAGGTGCAA ACGCCCTTCG ACACCCACAT TATGCAATAG CACTTGACTC CAGTGACCCT 
CTACTCTTTA CCACTGACCC TACGGAGCCC ACTGCCAAGG CAGAAAGTGC TATTTCTCAT 
CGCCGCACAG ATGTTAACCT TCCTCCACTA TCTATTGAGG ATGACTCCCT TGCATCAATT 
CCATTCATCG ATGAGCCGAC CAGTCCAAGT GCAGATCTGC GGGCACGTCA TGTCCCCGCC 
TCCTCCGTCG TGTCCAGTGG CGGTATAAAC TCCGCCCCTG CTGTGATCAC AAGCCCCGCC 
TCCCCAACCT TCACCTTTCC CCTCAACCGC CTTTTCTCCC ACAACTGCAG TGATCCTAAA 
TCTAATCGTC GCTCCTCGTA CCTGTTGGCC ATCACCACTG AACGCTCCAA GTCATGTGAT 
GAGGGGCTTA ACGCATATAG AGAAGAGGGC CGGGTCTTCT CGAGGCTTCC AAAAAGAGTC 
AAGAGCTTCT TTACTGAAGG GTCTCTGGAC AGTCTGGGTG CTGCTGAAGA GGCTCGCTCT 
AAACGACACT CTACCTCTGA GCTGGGCAAC ATCAGCTACA GTGACGTGAA GAGAGAAGGC 
TGGCTCCACT TCAAACAGAT CCACACGGAG AAGGGCAAGA AGGTTGGCAG TGCAATCTGG 
CCATGGAGAC GGGTGTTCTC TGTGCTTAGG ACCCATTCTC TGTTTCTTTA TAAAGACAAG 
CGAGAGGCAG TGCTTAAAGG GGCGCAATTA GGGGGCGGGG CTGAAGACGA GCAGCCAATC 
AGCATCCGGG GCTGCCTTGT GGATATAGCA TACAGTGAAA CGAAGCGCAA GCACGCGCTG 
AGATTGACCA CGCAGGACTT CTGCGAGTAC CTGCTGCAGG CGGAGGACAG AGAGGACATG 
CTGGAGTGGA TCCGAGTCAT CAGGGACAAC AGCAAGACCG ACAACGAGGA ATTGGGTTTC 
TCACGACAAG CTCTCATCAA TAAAAAGCTT AACGACTACA GGAAGCAAAG TCCAACGGGT 
AACAAACCAG ACTCCTCACC CAGAGTGCAT CGAATGACCT TCCTCCTCAG CAAGCCAGAC 
AACAGCGCAC CTCCACGCTC CCCAAAACAC GAGGCCAAAG ATGAGAGCAG CCCCCCAAAA 
TCTCCCTGGG GTATAAACAT AATGAAGAAG AGCAAGAAGG CCGGTCCTAA AGCTTTTGGC 
GTCCGTCTAG AGGACTGCCA GCCTGCAGTA AACAACAAGT TCATTCCTCA GATTGTGGAG 
ATCTGCTGTG GGCTGGTGGA GGACATGGGT CTGGAGTATA CAGGGATCTA CAGAGTGCCA 
GGAAACAATG CTGTAGTCTC CAGTCTGCAG GAGCAGCTCA ACAAAGGAGT AGACATTAAC 
ATCACTGAGG AGAAATGGCA GGACCTGAAT GTGGTGAGCA GCCTGCTGAA GTCCTTCTTT 
CGTAAACTCC CTGAACCCCT CTTCACTGAT GATAAATATA ATGACTTTAT CGACGCCAAT 
CGGATGGAGA ACGCCAGCGA TAGGCTGAGG ACAATGAAGA AATTGATTCG GGACCTACCA 
GATCATTACT TTCACACTCT GAAATTTTTA GTTGGACACC TAAAGACGGT GGCTGACCAC 
TCTGAGAAAA ACAAAATGGA GCCGCGTAAT TTGGCTCTGG TGTTTGGCCC CACTCTTGTT 
CGAACTTCAG AGGACAACAT GACAGACATG GTCACCCACA TGCCAGACCG CTACAAGATT 
GTGGAGACAC TCATTCAGCA TTACACCTGG TTTTTCTGCG AAGAACAGGA TAAGGATGAA 
AAGACCCCAG TGGACACAGA GGATGTCCAG CCTGCCCCAA ACATTGATCA TCTGCTCTCC 
AACATTGGCC GCACAGGCAT GCTGGGTGAG GCTTCAGACT CAACCAACAG TGATTCTGCA 
AAATCTAAGG GGTCATGGGG ATCAAAACGT GACTTTACTG CCAAGGATAT CCTTACATTA 
TCAATTATGT CGGCTGTGAC CCGCAAACGG CGGAAGCGGC ACAACGGCCG TCTCCTCGGC 
AGCAGCACAG ACGACGATTC TGAGCATGAA CCCATTAAAT CCTGCATCTC CGAGAAAGAC 
GATCTGCAAG AGATGGGGCA GGTTGCATCC TTCTGTGCCC CACGAGCAGA GGGAGAGGAG 
GATGACGATG AAGAGGAGGA AGATGATGAA GAGGAGGAGG AGGGGGCTGA GAGACAGAAG 
GCACAAGAGC AGAAGGAAAC AGTAGTCCCC AGCATTTCCT CAACAGAGGA AGTGACATCA 
ACAGTGCTGC TGAGCGAAGA GGAAGAGCAG AAGTCTGAAG TGAAAGGAGG ACGCAGCTGG 
AGATTAGATG ATGCTCGGTC TATTGTGTCA GGCTACTCTA CTCTGTCCAC TTTGGGGCGG 
AGCTTAGCTT CAGAGGGGCG GGGCGAGGAT GCAGATGATG AGCATAGTGA ACTAGTGAGT 
GAAACTGATA ATGAAAGCGG GTTTGCCTCA CGCTCCCTCA CCCAAGAAAG GCCAGAAAAA 
CCAAACCGTT CCAACCAGAA CTCTCATGCG TCTCCTGAAC CAACCGCACC ACAGAGTTTC 
CTCTATGCGC ACTGCAAATC ACCTACACCC TCTGGCTCCG CCACTGCTCC ATCTCCCCCT 
TCACAACACA TCACAACCAA CCCAGAGGAC AGCGCAGCAC GCTCGTCTAC TCCCTCAACC 
TCCTCTTACT CCTCATCCGC CTCACAACGG CTCCACAACC GCTCTTTCAA CTCCCACCGC 
CTCATACAGT GCGACACCCT TGCACGCCGT CGGACAAAGG CGGACAAAGC CAAAGCTCCC 
TCAGTGGACC TTTCCGAACT CTCGAGCTCC TCCGCCGCAG AGGAGGACCG CCAGTCAGGT 
GCCAATCAAA TCCCTGACTC CTCTCCGTCT CCTTCTTCAA AAGCCAAAGG GTTTAGCATA 
AGAAACGAGT GTACGCCAAT ATCCATGGTT GGTCCCGGAA GTAAGGGGTG TGTGTTGTCC 
CCCGGGCAAG GATCCCTGGC GGATCAGGTA CGTGCTCGTC TAATGGGCTC GGCGGATGAC 
CTGTGCAGCG TTGGCCTGAG AAAGCAGCTC TCGCCGGAGA CGCGCCGGAA GAGACGCGCT 
TGGAGGAGAC ACACAGTGGT CGTGTCCCCT ACGGATTGTT CAGACAAAAA GCCAACGGTC 
CAAAGCACCA AACCTCTAGC TTCACCCTCC CCTGTTGAAA AGCAGGATGT CAGAGAGTCC 
CACCCAGAGT GCTCAGAAAC TGAAGCCCCT ACTGCACGTC GGGCCGTGCC TACCTCGCGC 
TTTCGTGATT TCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap23a ( XM_003198148.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_003198196.2 Danio rerio Rho GTPase activating protein 23a (arhgap23a), transcript variant X1, mRNA.

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CloneID	ODa08640
Clone ID Related Accession (Same CDS sequence)	XM_003198148.5 , XM_003198148.4
Accession Version	XM_003198148.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	rho GTPase-activating protein 23 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_003198148.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCAG CAGGTCAAGT GCCACTGGCG ATGCCCGTTG TGTGCCCGCA TGTAGGGGGT 
AAGGAGTGGT GCTTTCAGGA TGCAGTCGGG GTGGATTGCA GCTCTCCAGA GCCTCGCTGT 
ATCTGGGTGG CGGTTTTACA CGGGGATACA CAGCAAATTA ACATTTCCAA CCTCCAAAAT 
GACTCTTCTC CAAACATAAA TGGCATCAGT GCTGGGAAAG CACTGTCAAG ACCCGCACAG 
ACCTCCAGGG CAAAAGGGCG GAGAGATGGG CTCTCTTCAT CCAGTGAGAA CCCTCGGCCG 
GTGGTCCGGA GCGGGACAGA TGGGCTGGGC GTGTCCTGGC AGGGTCCACG CACGCTGGTG 
CTCCAAAAGA ACTCTCAGGG CTTCGGCTTT ACTCTGCGTC ACTTCATCGT TTACCCGCCA 
GAGTCCGCTC TGCACACCAC TCTCAAGGAT GAAGAGAACG GGAATGGAAA GGGGCTTCAA 
CGAAGTCGTC TGGAGCCCAT GGACACAATT TTTGTGAAGA ATGTGAGGGA AAAAGGCCCC 
GCTCACCTGG CTGGCCTGTG TACAGGAGAC AGGCTGGTTA AAGTGAATGG GGAGAGCGTG 
CTGGGTAAGA CGTACTCGCA GGTCATTGCA CTGATTCAGA ACAGTGAGAG TGTACTGGAG 
CTCTCCATTA TGCCAAAGGA TGAGGACGTG CTTCAGCTGG CATATTCCCA TGATGCCTAC 
CTAAGGGGGA ACAATCCATA TACAGGAAAG GCCCAAAATC TTCCTCCACC ACCCCCTGTT 
CTCTACCCTC CTCGTACAAA ATCACAGTCT CCTGCAGGGT TCAGCCCCTG CCCTCCTCCA 
GCTTCTTCAC ACATGGGGCA AAACCAGCTG GACAACTGGA GCCGCTGGCC CGGCTCTTCT 
GCTGGTCCCT CTCCACCCCT TGACAACCGG ACTGTGGCCT CCAGCAATGT TGTCTGGGCA 
GCCGAGGGCA GGAATTGTGA GCCGGGTGGT GTTGGTCACA GTAGCCCTGC ACACCGAACA 
GAGGAGATCC AGTATGGAAT GACTGACAGA GGACAGCAGC CAACTGGACA AATGAGAGGA 
CGCTCATACT CATCTTCTTC CTCCTCAGGA GGAATGATGA GCCCTTTGCA TCATGGTCAT 
CTTGCCAATC ACAACATGGG TACTGCCATG GGGCACACTG GAGGTCGTAA GGGAAGCCCA 
CCTTGGGCCA GCCCTTCTCA GCCAACATCA ACACCTTCAT CCAGCCGCAA CGAACGAACC 
CAACAAGCCC TTTCCAACTG GTACTACAGC CAGGTCCCAG GCCGTGAGCG AGAACGAGAA 
CGAGAGCGGG AGCGATCTAG TTCTCGCTCA ATGCACCCAC GCCACAGGAG TTTCTCACAA 
GACCGTCTGG GAGAGCTTGG GAAGGCCAGG CGTTCTCAAC AAGCAGGTTT TCCCCATAGT 
GCATCGCAAG ACACGTTGCT GCTGCTTCAG CAGAGTGCTG CAGCATATGC TGATCCTTAC 
TGGAACCATG GAGACTGGCG GGGTGAGCAT ATGTATGGTC AGCACCCATC TGTGGTGGCC 
TATGGGCGAA AGTCCTCCGA AAATCTTCTA GCAAGCCGGT ACGGACACTC GGGACGCTCA 
CTGGAAAATC TCGACAGAGC TGTGGGGATA CTCTCACCAA GGGTTGAAAG ATCTGCTTGG 
GTTCAACAAC CTCCAAATAG GACTGAAGTC TATGCAAGAC ATGCTGGGCA TTTCGGCCAA 
CATATAGAGT CACCCCACCA AATGCACCAC ACACCCCAAC ACCACCAGAA TCTCACACAT 
GCAACACACC AACACTCATC TCAAATCCCA CAACCTCAGC AGGCAGCTCC AATGGCAAGA 
CGTTTGCCTT CTTGCCAGAG TCTAGATGAG GAGCCGATTG GCTATCGTAG CTACAGTCCC 
TCATTCAATA GGAAGACTGG CCGTATCTTG CAGCAGGCCC AGTCATTTAG AGATCCCTCT 
TACACAGGTC CACACCTTTG GACTCCAACA TCCAAAACCA GTCCCCCAGA GGGGCATCCA 
CCCTCAGCCA CTGCTTCCCC AGCCCCACCA ACCACTTCCT CTTCAACTAC GATTTCCACA 
CCATCTGAAG GACAAGACAG AACTTACCGA CCCACTAATC ATGACAGAGG GGCAGGGGAA 
GCGACAAGTG AAGCCCAGAC ACAGGAAGTG GTTCTGAGGC AGAAACCACC TACCGGCCGA 
CGAGGTGCAA ACGCCCTTCG ACACCCACAT TATGCAATAG CACTTGACTC CAGTGACCCT 
CTACTCTTTA CCACTGACCC TACGGAGCCC ACTGCCAAGG CAGAAAGTGC TATTTCTCAT 
CGCCGCACAG ATGTTAACCT TCCTCCACTA TCTATTGAGG ATGACTCCCT TGCATCAATT 
CCATTCATCG ATGAGCCGAC CAGTCCAAGT GCAGATCTGC GGGCACGTCA TGTCCCCGCC 
TCCTCCGTCG TGTCCAGTGG CGGTATAAAC TCCGCCCCTG CTGTGATCAC AAGCCCCGCC 
TCCCCAACCT TCACCTTTCC CCTCAACCGC CTTTTCTCCC ACAACTGCAG TGATCCTAAA 
TCTAATCGTC GCTCCTCGTA CCTGTTGGCC ATCACCACTG AACGCTCCAA GTCATGTGAT 
GAGGGGCTTA ACGCATATAG AGAAGAGGGC CGGGTCTTCT CGAGGCTTCC AAAAAGAGTC 
AAGAGCTTCT TTACTGAAGG GTCTCTGGAC AGTCTGGGTG CTGCTGAAGA GGCTCGCTCT 
AAACGACACT CTACCTCTGA GCTGGGCAAC ATCAGCTACA GTGACGTGAA GAGAGAAGGC 
TGGCTCCACT TCAAACAGAT CCACACGGAG AAGGGCAAGA AGGTTGGCAG TGCAATCTGG 
CCATGGAGAC GGGTGTTCTC TGTGCTTAGG ACCCATTCTC TGTTTCTTTA TAAAGACAAG 
CGAGAGGCAG TGCTTAAAGG GGCGCAATTA GGGGGCGGGG CTGAAGACGA GCAGCCAATC 
AGCATCCGGG GCTGCCTTGT GGATATAGCA TACAGTGAAA CGAAGCGCAA GCACGCGCTG 
AGATTGACCA CGCAGGACTT CTGCGAGTAC CTGCTGCAGG CGGAGGACAG AGAGGACATG 
CTGGAGTGGA TCCGAGTCAT CAGGGACAAC AGCAAGACCG ACAACGAGGA ATTGGGTTTC 
TCACGACAAG CTCTCATCAA TAAAAAGCTT AACGACTACA GGAAGCAAAG TCCAACGGGT 
AACAAACCAG ACTCCTCACC CAGAGTGCAT CGAATGACCT TCCTCCTCAG CAAGCCAGAC 
AACAGCGCAC CTCCACGCTC CCCAAAACAC GAGGCCAAAG ATGAGAGCAG CCCCCCAAAA 
TCTCCCTGGG GTATAAACAT AATGAAGAAG AGCAAGAAGG CCGGTCCTAA AGCTTTTGGC 
GTCCGTCTAG AGGACTGCCA GCCTGCAGTA AACAACAAGT TCATTCCTCA GATTGTGGAG 
ATCTGCTGTG GGCTGGTGGA GGACATGGGT CTGGAGTATA CAGGGATCTA CAGAGTGCCA 
GGAAACAATG CTGTAGTCTC CAGTCTGCAG GAGCAGCTCA ACAAAGGAGT AGACATTAAC 
ATCACTGAGG AGAAATGGCA GGACCTGAAT GTGGTGAGCA GCCTGCTGAA GTCCTTCTTT 
CGTAAACTCC CTGAACCCCT CTTCACTGAT GATAAATATA ATGACTTTAT CGACGCCAAT 
CGGATGGAGA ACGCCAGCGA TAGGCTGAGG ACAATGAAGA AATTGATTCG GGACCTACCA 
GATCATTACT TTCACACTCT GAAATTTTTA GTTGGACACC TAAAGACGGT GGCTGACCAC 
TCTGAGAAAA ACAAAATGGA GCCGCGTAAT TTGGCTCTGG TGTTTGGCCC CACTCTTGTT 
CGAACTTCAG AGGACAACAT GACAGACATG GTCACCCACA TGCCAGACCG CTACAAGATT 
GTGGAGACAC TCATTCAGCA TTACACCTGG TTTTTCTGCG AAGAACAGGA TAAGGATGAA 
AAGACCCCAG TGGACACAGA GGATGTCCAG CCTGCCCCAA ACATTGATCA TCTGCTCTCC 
AACATTGGCC GCACAGGCAT GCTGGGTGAG GCTTCAGACT CAACCAACAG TGATTCTGCA 
AAATCTAAGG GGTCATGGGG ATCAAAACGT GACTTTACTG CCAAGGATAT CCTTACATTA 
TCAATTATGT CGGCTGTGAC CCGCAAACGG CGGAAGCGGC ACAACGGCCG TCTCCTCGGC 
AGCAGCACAG ACGACGATTC TGAGCATGAA CCCATTAAAT CCTGCATCTC CGAGAAAGAC 
GATCTGCAAG AGATGGGGCA GGTTGCATCC TTCTGTGCCC CACGAGCAGA GGGAGAGGAG 
GATGACGATG AAGAGGAGGA AGATGATGAA GAGGAGGAGG AGGGGGCTGA GAGACAGAAG 
GCACAAGAGC AGAAGGAAAC AGTAGTCCCC AGCATTTCCT CAACAGAGGA AGTGACATCA 
ACAGTGCTGC TGAGCGAAGA GGAAGAGCAG AAGTCTGAAG TGAAAGGAGG ACGCAGCTGG 
AGATTAGATG ATGCTCGGTC TATTGTGTCA GGCTACTCTA CTCTGTCCAC TTTGGGGCGG 
AGCTTAGCTT CAGAGGGGCG GGGCGAGGAT GCAGATGATG AGCATAGTGA ACTAGTGAGT 
GAAACTGATA ATGAAAGCGG GTTTGCCTCA CGCTCCCTCA CCCAAGAAAG GCCAGAAAAA 
CCAAACCGTT CCAACCAGAA CTCTCATGCG TCTCCTGAAC CAACCGCACC ACAGAGTTTC 
CTCTATGCGC ACTGCAAATC ACCTACACCC TCTGGCTCCG CCACTGCTCC ATCTCCCCCT 
TCACAACACA TCACAACCAA CCCAGAGGAC AGCGCAGCAC GCTCGTCTAC TCCCTCAACC 
TCCTCTTACT CCTCATCCGC CTCACAACGG CTCCACAACC GCTCTTTCAA CTCCCACCGC 
CTCATACAGT GCGACACCCT TGCACGCCGT CGGACAAAGG CGGACAAAGC CAAAGCTCCC 
TCAGTGGACC TTTCCGAACT CTCGAGCTCC TCCGCCGCAG AGGAGGACCG CCAGTCAGGT 
GCCAATCAAA TCCCTGACTC CTCTCCGTCT CCTTCTTCAA AAGCCAAAGG GTTTAGCATA 
AGAAACGAGT GTACGCCAAT ATCCATGGTT GGTCCCGGAA GTAAGGGGTG TGTGTTGTCC 
CCCGGGCAAG GATCCCTGGC GGATCAGGTA CGTGCTCGTC TAATGGGCTC GGCGGATGAC 
CTGTGCAGCG TTGGCCTGAG AAAGCAGCTC TCGCCGGAGA CGCGCCGGAA GAGACGCGCT 
TGGAGGAGAC ACACAGTGGT CGTGTCCCCT ACGGATTGTT CAGACAAAAA GCCAACGGTC 
CAAAGCACCA AACCTCTAGC TTCACCCTCC CCTGTTGAAA AGCAGGATGT CAGAGAGTCC 
CACCCAGAGT GCTCAGAAAC TGAAGCCCCT ACTGCACGTC GGGCCGTGCC TACCTCGCGC 
TTTCGTGATT TCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003198196.2
CDS	1017..6554
Translation	MWSAGQVPLAMPVVCPHVGGKEWCFQDAVGVDCSSPEPRCIWVAVLHGDTQQINISNLQNDSSPNINGISAGKALSRPAQTSRAKGRRDGLSSSSENPRPVVRSGTDGLGVSWQGPRTLVLQKNSQGFGFTLRHFIVYPPESALHTTLKDEENGNGKGLQRSRLEPMDTIFVKNVREKGPAHLAGLCTGDRLVKVNGESVLGKTYSQVIALIQNSESVLELSIMPKDEDVLQLAYSHDAYLRGNNPYTGKAQNLPPPPPVLYPPRTKSQSPAGFSPCPPPASSHMGQNQLDNWSRWPGSSAGPSPPLDNRTVASSNVVWAAEGRNCEPGGVGHSSPAHRTEEIQYGMTDRGQQPTGQMRGRSYSSSSSSGGMMSPLHHGHLANHNMGTAMGHTGGRKGSPPWASPSQPTSTPSSSRNERTQQALSNWYYSQVPGRERERERERERSSSRSMHPRHRSFSQDRLGELGKARRSQQAGFPHSASQDTLLLLQQSAAAYADPYWNHGDWRGEHMYGQHPSVVAYGRKSSENLLASRYGHSGRSLENLDRAVGILSPRVERSAWVQQPPNRTEVYARHAGHFGQHIESPHQMHHTPQHHQNLTHATHQHSSQIPQPQQAAPMARRLPSCQSLDEEPIGYRSYSPSFNRKTGRILQQAQSFRDPSYTGPHLWTPTSKTSPPEGHPPSATASPAPPTTSSSTTISTPSEGQDRTYRPTNHDRGAGEATSEAQTQEVVLRQKPPTGRRGANALRHPHYAIALDSSDPLLFTTDPTEPTAKAESAISHRRTDVNLPPLSIEDDSLASIPFIDEPTSPSADLRARHVPASSVVSSGGINSAPAVITSPASPTFTFPLNRLFSHNCSDPKSNRRSSYLLAITTERSKSCDEGLNAYREEGRVFSRLPKRVKSFFTEGSLDSLGAAEEARSKRHSTSELGNISYSDVKREGWLHFKQIHTEKGKKVGSAIWPWRRVFSVLRTHSLFLYKDKREAVLKGAQLGGGAEDEQPISIRGCLVDIAYSETKRKHALRLTTQDFCEYLLQAEDREDMLEWIRVIRDNSKTDNEELGFSRQALINKKLNDYRKQSPTGNKPDSSPRVHRMTFLLSKPDNSAPPRSPKHEAKDESSPPKSPWGINIMKKSKKAGPKAFGVRLEDCQPAVNNKFIPQIVEICCGLVEDMGLEYTGIYRVPGNNAVVSSLQEQLNKGVDINITEEKWQDLNVVSSLLKSFFRKLPEPLFTDDKYNDFIDANRMENASDRLRTMKKLIRDLPDHYFHTLKFLVGHLKTVADHSEKNKMEPRNLALVFGPTLVRTSEDNMTDMVTHMPDRYKIVETLIQHYTWFFCEEQDKDEKTPVDTEDVQPAPNIDHLLSNIGRTGMLGEASDSTNSDSAKSKGSWGSKRDFTAKDILTLSIMSAVTRKRRKRHNGRLLGSSTDDDSEHEPIKSCISEKDDLQEMGQVASFCAPRAEGEEDDDEEEEDDEEEEEGAERQKAQEQKETVVPSISSTEEVTSTVLLSEEEEQKSEVKGGRSWRLDDARSIVSGYSTLSTLGRSLASEGRGEDADDEHSELVSETDNESGFASRSLTQERPEKPNRSNQNSHASPEPTAPQSFLYAHCKSPTPSGSATAPSPPSQHITTNPEDSAARSSTPSTSSYSSSASQRLHNRSFNSHRLIQCDTLARRRTKADKAKAPSVDLSELSSSSAAEEDRQSGANQIPDSSPSPSSKAKGFSIRNECTPISMVGPGSKGCVLSPGQGSLADQVRARLMGSADDLCSVGLRKQLSPETRRKRRAWRRHTVVVSPTDCSDKKPTVQSTKPLASPSPVEKQDVRESHPECSETEAPTARRAVPTSRFRDFL

Target ORF information:

RefSeq Version	XM_003198148.4
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Rho GTPase activating protein 23a (arhgap23a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003198148.4

ORF Insert Sequence:

ATGTGGTCAG CAGGTCAAGT GCCACTGGCG ATGCCCGTTG TGTGCCCGCA TGTAGGGGGT 
AAGGAGTGGT GCTTTCAGGA TGCAGTCGGG GTGGATTGCA GCTCTCCAGA GCCTCGCTGT 
ATCTGGGTGG CGGTTTTACA CGGGGATACA CAGCAAATTA ACATTTCCAA CCTCCAAAAT 
GACTCTTCTC CAAACATAAA TGGCATCAGT GCTGGGAAAG CACTGTCAAG ACCCGCACAG 
ACCTCCAGGG CAAAAGGGCG GAGAGATGGG CTCTCTTCAT CCAGTGAGAA CCCTCGGCCG 
GTGGTCCGGA GCGGGACAGA TGGGCTGGGC GTGTCCTGGC AGGGTCCACG CACGCTGGTG 
CTCCAAAAGA ACTCTCAGGG CTTCGGCTTT ACTCTGCGTC ACTTCATCGT TTACCCGCCA 
GAGTCCGCTC TGCACACCAC TCTCAAGGAT GAAGAGAACG GGAATGGAAA GGGGCTTCAA 
CGAAGTCGTC TGGAGCCCAT GGACACAATT TTTGTGAAGA ATGTGAGGGA AAAAGGCCCC 
GCTCACCTGG CTGGCCTGTG TACAGGAGAC AGGCTGGTTA AAGTGAATGG GGAGAGCGTG 
CTGGGTAAGA CGTACTCGCA GGTCATTGCA CTGATTCAGA ACAGTGAGAG TGTACTGGAG 
CTCTCCATTA TGCCAAAGGA TGAGGACGTG CTTCAGCTGG CATATTCCCA TGATGCCTAC 
CTAAGGGGGA ACAATCCATA TACAGGAAAG GCCCAAAATC TTCCTCCACC ACCCCCTGTT 
CTCTACCCTC CTCGTACAAA ATCACAGTCT CCTGCAGGGT TCAGCCCCTG CCCTCCTCCA 
GCTTCTTCAC ACATGGGGCA AAACCAGCTG GACAACTGGA GCCGCTGGCC CGGCTCTTCT 
GCTGGTCCCT CTCCACCCCT TGACAACCGG ACTGTGGCCT CCAGCAATGT TGTCTGGGCA 
GCCGAGGGCA GGAATTGTGA GCCGGGTGGT GTTGGTCACA GTAGCCCTGC ACACCGAACA 
GAGGAGATCC AGTATGGAAT GACTGACAGA GGACAGCAGC CAACTGGACA AATGAGAGGA 
CGCTCATACT CATCTTCTTC CTCCTCAGGA GGAATGATGA GCCCTTTGCA TCATGGTCAT 
CTTGCCAATC ACAACATGGG TACTGCCATG GGGCACACTG GAGGTCGTAA GGGAAGCCCA 
CCTTGGGCCA GCCCTTCTCA GCCAACATCA ACACCTTCAT CCAGCCGCAA CGAACGAACC 
CAACAAGCCC TTTCCAACTG GTACTACAGC CAGGTCCCAG GCCGTGAGCG AGAACGAGAA 
CGAGAGCGGG AGCGATCTAG TTCTCGCTCA ATGCACCCAC GCCACAGGAG TTTCTCACAA 
GACCGTCTGG GAGAGCTTGG GAAGGCCAGG CGTTCTCAAC AAGCAGGTTT TCCCCATAGT 
GCATCGCAAG ACACGTTGCT GCTGCTTCAG CAGAGTGCTG CAGCATATGC TGATCCTTAC 
TGGAACCATG GAGACTGGCG GGGTGAGCAT ATGTATGGTC AGCACCCATC TGTGGTGGCC 
TATGGGCGAA AGTCCTCCGA AAATCTTCTA GCAAGCCGGT ACGGACACTC GGGACGCTCA 
CTGGAAAATC TCGACAGAGC TGTGGGGATA CTCTCACCAA GGGTTGAAAG ATCTGCTTGG 
GTTCAACAAC CTCCAAATAG GACTGAAGTC TATGCAAGAC ATGCTGGGCA TTTCGGCCAA 
CATATAGAGT CACCCCACCA AATGCACCAC ACACCCCAAC ACCACCAGAA TCTCACACAT 
GCAACACACC AACACTCATC TCAAATCCCA CAACCTCAGC AGGCAGCTCC AATGGCAAGA 
CGTTTGCCTT CTTGCCAGAG TCTAGATGAG GAGCCGATTG GCTATCGTAG CTACAGTCCC 
TCATTCAATA GGAAGACTGG CCGTATCTTG CAGCAGGCCC AGTCATTTAG AGATCCCTCT 
TACACAGGTC CACACCTTTG GACTCCAACA TCCAAAACCA GTCCCCCAGA GGGGCATCCA 
CCCTCAGCCA CTGCTTCCCC AGCCCCACCA ACCACTTCCT CTTCAACTAC GATTTCCACA 
CCATCTGAAG GACAAGACAG AACTTACCGA CCCACTAATC ATGACAGAGG GGCAGGGGAA 
GCGACAAGTG AAGCCCAGAC ACAGGAAGTG GTTCTGAGGC AGAAACCACC TACCGGCCGA 
CGAGGTGCAA ACGCCCTTCG ACACCCACAT TATGCAATAG CACTTGACTC CAGTGACCCT 
CTACTCTTTA CCACTGACCC TACGGAGCCC ACTGCCAAGG CAGAAAGTGC TATTTCTCAT 
CGCCGCACAG ATGTTAACCT TCCTCCACTA TCTATTGAGG ATGACTCCCT TGCATCAATT 
CCATTCATCG ATGAGCCGAC CAGTCCAAGT GCAGATCTGC GGGCACGTCA TGTCCCCGCC 
TCCTCCGTCG TGTCCAGTGG CGGTATAAAC TCCGCCCCTG CTGTGATCAC AAGCCCCGCC 
TCCCCAACCT TCACCTTTCC CCTCAACCGC CTTTTCTCCC ACAACTGCAG TGATCCTAAA 
TCTAATCGTC GCTCCTCGTA CCTGTTGGCC ATCACCACTG AACGCTCCAA GTCATGTGAT 
GAGGGGCTTA ACGCATATAG AGAAGAGGGC CGGGTCTTCT CGAGGCTTCC AAAAAGAGTC 
AAGAGCTTCT TTACTGAAGG GTCTCTGGAC AGTCTGGGTG CTGCTGAAGA GGCTCGCTCT 
AAACGACACT CTACCTCTGA GCTGGGCAAC ATCAGCTACA GTGACGTGAA GAGAGAAGGC 
TGGCTCCACT TCAAACAGAT CCACACGGAG AAGGGCAAGA AGGTTGGCAG TGCAATCTGG 
CCATGGAGAC GGGTGTTCTC TGTGCTTAGG ACCCATTCTC TGTTTCTTTA TAAAGACAAG 
CGAGAGGCAG TGCTTAAAGG GGCGCAATTA GGGGGCGGGG CTGAAGACGA GCAGCCAATC 
AGCATCCGGG GCTGCCTTGT GGATATAGCA TACAGTGAAA CGAAGCGCAA GCACGCGCTG 
AGATTGACCA CGCAGGACTT CTGCGAGTAC CTGCTGCAGG CGGAGGACAG AGAGGACATG 
CTGGAGTGGA TCCGAGTCAT CAGGGACAAC AGCAAGACCG ACAACGAGGA ATTGGGTTTC 
TCACGACAAG CTCTCATCAA TAAAAAGCTT AACGACTACA GGAAGCAAAG TCCAACGGGT 
AACAAACCAG ACTCCTCACC CAGAGTGCAT CGAATGACCT TCCTCCTCAG CAAGCCAGAC 
AACAGCGCAC CTCCACGCTC CCCAAAACAC GAGGCCAAAG ATGAGAGCAG CCCCCCAAAA 
TCTCCCTGGG GTATAAACAT AATGAAGAAG AGCAAGAAGG CCGGTCCTAA AGCTTTTGGC 
GTCCGTCTAG AGGACTGCCA GCCTGCAGTA AACAACAAGT TCATTCCTCA GATTGTGGAG 
ATCTGCTGTG GGCTGGTGGA GGACATGGGT CTGGAGTATA CAGGGATCTA CAGAGTGCCA 
GGAAACAATG CTGTAGTCTC CAGTCTGCAG GAGCAGCTCA ACAAAGGAGT AGACATTAAC 
ATCACTGAGG AGAAATGGCA GGACCTGAAT GTGGTGAGCA GCCTGCTGAA GTCCTTCTTT 
CGTAAACTCC CTGAACCCCT CTTCACTGAT GATAAATATA ATGACTTTAT CGACGCCAAT 
CGGATGGAGA ACGCCAGCGA TAGGCTGAGG ACAATGAAGA AATTGATTCG GGACCTACCA 
GATCATTACT TTCACACTCT GAAATTTTTA GTTGGACACC TAAAGACGGT GGCTGACCAC 
TCTGAGAAAA ACAAAATGGA GCCGCGTAAT TTGGCTCTGG TGTTTGGCCC CACTCTTGTT 
CGAACTTCAG AGGACAACAT GACAGACATG GTCACCCACA TGCCAGACCG CTACAAGATT 
GTGGAGACAC TCATTCAGCA TTACACCTGG TTTTTCTGCG AAGAACAGGA TAAGGATGAA 
AAGACCCCAG TGGACACAGA GGATGTCCAG CCTGCCCCAA ACATTGATCA TCTGCTCTCC 
AACATTGGCC GCACAGGCAT GCTGGGTGAG GCTTCAGACT CAACCAACAG TGATTCTGCA 
AAATCTAAGG GGTCATGGGG ATCAAAACGT GACTTTACTG CCAAGGATAT CCTTACATTA 
TCAATTATGT CGGCTGTGAC CCGCAAACGG CGGAAGCGGC ACAACGGCCG TCTCCTCGGC 
AGCAGCACAG ACGACGATTC TGAGCATGAA CCCATTAAAT CCTGCATCTC CGAGAAAGAC 
GATCTGCAAG AGATGGGGCA GGTTGCATCC TTCTGTGCCC CACGAGCAGA GGGAGAGGAG 
GATGACGATG AAGAGGAGGA AGATGATGAA GAGGAGGAGG AGGGGGCTGA GAGACAGAAG 
GCACAAGAGC AGAAGGAAAC AGTAGTCCCC AGCATTTCCT CAACAGAGGA AGTGACATCA 
ACAGTGCTGC TGAGCGAAGA GGAAGAGCAG AAGTCTGAAG TGAAAGGAGG ACGCAGCTGG 
AGATTAGATG ATGCTCGGTC TATTGTGTCA GGCTACTCTA CTCTGTCCAC TTTGGGGCGG 
AGCTTAGCTT CAGAGGGGCG GGGCGAGGAT GCAGATGATG AGCATAGTGA ACTAGTGAGT 
GAAACTGATA ATGAAAGCGG GTTTGCCTCA CGCTCCCTCA CCCAAGAAAG GCCAGAAAAA 
CCAAACCGTT CCAACCAGAA CTCTCATGCG TCTCCTGAAC CAACCGCACC ACAGAGTTTC 
CTCTATGCGC ACTGCAAATC ACCTACACCC TCTGGCTCCG CCACTGCTCC ATCTCCCCCT 
TCACAACACA TCACAACCAA CCCAGAGGAC AGCGCAGCAC GCTCGTCTAC TCCCTCAACC 
TCCTCTTACT CCTCATCCGC CTCACAACGG CTCCACAACC GCTCTTTCAA CTCCCACCGC 
CTCATACAGT GCGACACCCT TGCACGCCGT CGGACAAAGG CGGACAAAGC CAAAGCTCCC 
TCAGTGGACC TTTCCGAACT CTCGAGCTCC TCCGCCGCAG AGGAGGACCG CCAGTCAGGT 
GCCAATCAAA TCCCTGACTC CTCTCCGTCT CCTTCTTCAA AAGCCAAAGG GTTTAGCATA 
AGAAACGAGT GTACGCCAAT ATCCATGGTT GGTCCCGGAA GTAAGGGGTG TGTGTTGTCC 
CCCGGGCAAG GATCCCTGGC GGATCAGGTA CGTGCTCGTC TAATGGGCTC GGCGGATGAC 
CTGTGCAGCG TTGGCCTGAG AAAGCAGCTC TCGCCGGAGA CGCGCCGGAA GAGACGCGCT 
TGGAGGAGAC ACACAGTGGT CGTGTCCCCT ACGGATTGTT CAGACAAAAA GCCAACGGTC 
CAAAGCACCA AACCTCTAGC TTCACCCTCC CCTGTTGAAA AGCAGGATGT CAGAGAGTCC 
CACCCAGAGT GCTCAGAAAC TGAAGCCCCT ACTGCACGTC GGGCCGTGCC TACCTCGCGC 
TTTCGTGATT TCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap23a ( XM_005164174.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005164231.1 Danio rerio Rho GTPase activating protein 23a (arhgap23a), transcript variant X2, mRNA.

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CloneID	ODa08641
Clone ID Related Accession (Same CDS sequence)	XM_005164174.3 , XM_005164174.4
Accession Version	XM_005164174.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5379bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	rho GTPase-activating protein 23 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005164174.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCAG CAGGTCAAGT GCCACTGGCG ATGCCCGTTG TGTGCCCGCA TGTAGGGGGT 
AAGGAGTGGT GCTTTCAGGA TGCAGTCGGG GTGGATTGCA GCTCTCCAGA GCCTCGCTGT 
ATCTGGGTGG CGGTTTTACA CGGGGATACA CAGCAAATTA ACATTTCCAA CCTCCAAAAT 
GACTCTTCTC CAAACATAAA TGGCATCAGT GCTGGGAAAG CACTGTCAAG ACCCGCACAG 
ACCTCCAGGG CAAAAGGGCG GAGAGATGGG CTCTCTTCAT CCAGTGAGAA CCCTCGGCCG 
GTGGTCCGGA GCGGGACAGA TGGGCTGGGC GTGTCCTGGC AGGGTCCACG CACGCTGGTG 
CTCCAAAAGA ACTCTCAGGG CTTCGGCTTT ACTCTGCGTC ACTTCATCGT TTACCCGCCA 
GAGTCCGCTC TGCACACCAC TCTCAAGGAT GAAGAGAACG GGAATGGAAA GGGGCTTCAA 
CGAAGTCGTC TGGAGCCCAT GGACACAATT TTTGTGAAGA ATGTGAGGGA AAAAGGCCCC 
GCTCACCTGG CTGGCCTGTG TACAGGAGAC AGGCTGGTTA AAGTGAATGG GGAGAGCGTG 
CTGGGTAAGA CGTACTCGCA GGTCATTGCA CTGATTCAGA ACAGTGAGAG TGTACTGGAG 
CTCTCCATTA TGCCAAAGGA TGAGGACGTG CTTCAGCTGG CATATTCCCA TGATGCCTAC 
CTAAGGGGGA ACAATCCATA TACAGGAAAG GCCCAAAATC TTCCTCCACC ACCCCCTGTT 
CTCTACCCTC CTCGTACAAA ATCACAGTCT CCTGCAGGGT TCAGCCCCTG CCCTCCTCCA 
GCTTCTTCAC ACATGGGGCA AAACCAGCTG GACAACTGGA GCCGCTGGCC CGGCTCTTCT 
GCTGGTCCCT CTCCACCCCT TGACAACCGG ACTGTGGCCT CCAGCAATGT TGTCTGGGCA 
GCCGAGGGCA GGAATTGTGA GCCGGGTGGT GTTGGTCACA GTAGCCCTGC ACACCGAACA 
GAGGAGATCC AGTATGGAAT GACTGACAGA GGACAGCAGC CAACTGGACA AATGAGAGGA 
CGCTCATACT CATCTTCTTC CTCCTCAGGA GGAATGATGA GCCCTTTGCA TCATGGTCAT 
CTTGCCAATC ACAACATGGG TACTGCCATG GGGCACACTG GAGGTCGTAA GGGAAGCCCA 
CCTTGGGCCA GCCCTTCTCA GCCAACATCA ACACCTTCAT CCAGCCGCAA CGAACGAACC 
CAACAAGCCC TTTCCAACTG GTACTACAGC CAGGTCCCAG GCCGTGAGCG AGAACGAGAA 
CGAGAGCGGG AGCGATCTAG TTCTCGCTCA ATGCACCCAC GCCACAGGAG TTTCTCACAA 
GACCGTCTGG GAGAGCTTGG GAAGGCCAGG CGTTCTCAAC AAGCAGGTTT TCCCCATAGT 
GCATCGCAAG ACACGTTGCT GCTGCTTCAG CAGAGTGCTG CAGCATATGC TGATCCTTAC 
TGGAACCATG GAGACTGGCG GGGTGAGCAT ATGTATGGTC AGCACCCATC TGTGGTGGCC 
TATGGGCGAA AGTCCTCCGA AAATCTTCTA GCAAGCCGGT ACGGACACTC GGGACGCTCA 
CTGGAAAATC TCGACAGAGC TGTGGGGATA CTCTCACCAA GGGTTGAAAG ATCTGCTTGG 
GTTCAACAAC CTCCAAATAG GACTGAAGTC TATGCAAGAC ATGCTGGGCA TTTCGGCCAA 
CATATAGAGT CACCCCACCA AATGCACCAC ACACCCCAAC ACCACCAGAA TCTCACACAT 
GCAACACACC AACACTCATC TCAAATCCCA CAACCTCAGC AGGCAGCTCC AATGGCAAGA 
CGTTTGCCTT CTTGCCAGAG TCTAGATGAG GAGCCGATTG GCTATCGTAG CTACAGTCCC 
TCATTCAATA GGAAGACTGG CCGTATCTTG CAGCAGGCCC AGTCATTTAG AGATCCCTCT 
TACACAGGTC CACACCTTTG GACTCCAACA TCCAAAACCA GTCCCCCAGA GGGGCATCCA 
CCCTCAGCCA CTGCTTCCCC AGCCCCACCA ACCACTTCCT CTTCAACTAC GATTTCCACA 
CCATCTGAAG GACAAGACAG AACTTACCGA CCCACTAATC ATGACAGAGG GGCAGGGGAA 
GCGACAAGTG AAGCCCAGAC ACAGGAAGTG GTTCTGAGGC AGAAACCACC TACCGGCCGA 
CGAGGTGCAA ACGCCCTTCG ACACCCACAT TATGCAATAG CACTTGACTC CAGTGACCCT 
CTACTCTTTA CCACTGACCC TACGGAGCCC ACTGCCAAGG CAGAAAGTGC TATTTCTCAT 
CGCCGCACAG ATGTTAACCT TCCTCCACTA TCTATTGAGG ATGACTCCCT TGCATCAATT 
CCATTCATCG GTGATCCTAA ATCTAATCGT CGCTCCTCGT ACCTGTTGGC CATCACCACT 
GAACGCTCCA AGTCATGTGA TGAGGGGCTT AACGCATATA GAGAAGAGGG CCGGGTCTTC 
TCGAGGCTTC CAAAAAGAGT CAAGAGCTTC TTTACTGAAG GGTCTCTGGA CAGTCTGGGT 
GCTGCTGAAG AGGCTCGCTC TAAACGACAC TCTACCTCTG AGCTGGGCAA CATCAGCTAC 
AGTGACGTGA AGAGAGAAGG CTGGCTCCAC TTCAAACAGA TCCACACGGA GAAGGGCAAG 
AAGGTTGGCA GTGCAATCTG GCCATGGAGA CGGGTGTTCT CTGTGCTTAG GACCCATTCT 
CTGTTTCTTT ATAAAGACAA GCGAGAGGCA GTGCTTAAAG GGGCGCAATT AGGGGGCGGG 
GCTGAAGACG AGCAGCCAAT CAGCATCCGG GGCTGCCTTG TGGATATAGC ATACAGTGAA 
ACGAAGCGCA AGCACGCGCT GAGATTGACC ACGCAGGACT TCTGCGAGTA CCTGCTGCAG 
GCGGAGGACA GAGAGGACAT GCTGGAGTGG ATCCGAGTCA TCAGGGACAA CAGCAAGACC 
GACAACGAGG AATTGGGTTT CTCACGACAA GCTCTCATCA ATAAAAAGCT TAACGACTAC 
AGGAAGCAAA GTCCAACGGG TAACAAACCA GACTCCTCAC CCAGAGTGCA TCGAATGACC 
TTCCTCCTCA GCAAGCCAGA CAACAGCGCA CCTCCACGCT CCCCAAAACA CGAGGCCAAA 
GATGAGAGCA GCCCCCCAAA ATCTCCCTGG GGTATAAACA TAATGAAGAA GAGCAAGAAG 
GCCGGTCCTA AAGCTTTTGG CGTCCGTCTA GAGGACTGCC AGCCTGCAGT AAACAACAAG 
TTCATTCCTC AGATTGTGGA GATCTGCTGT GGGCTGGTGG AGGACATGGG TCTGGAGTAT 
ACAGGGATCT ACAGAGTGCC AGGAAACAAT GCTGTAGTCT CCAGTCTGCA GGAGCAGCTC 
AACAAAGGAG TAGACATTAA CATCACTGAG GAGAAATGGC AGGACCTGAA TGTGGTGAGC 
AGCCTGCTGA AGTCCTTCTT TCGTAAACTC CCTGAACCCC TCTTCACTGA TGATAAATAT 
AATGACTTTA TCGACGCCAA TCGGATGGAG AACGCCAGCG ATAGGCTGAG GACAATGAAG 
AAATTGATTC GGGACCTACC AGATCATTAC TTTCACACTC TGAAATTTTT AGTTGGACAC 
CTAAAGACGG TGGCTGACCA CTCTGAGAAA AACAAAATGG AGCCGCGTAA TTTGGCTCTG 
GTGTTTGGCC CCACTCTTGT TCGAACTTCA GAGGACAACA TGACAGACAT GGTCACCCAC 
ATGCCAGACC GCTACAAGAT TGTGGAGACA CTCATTCAGC ATTACACCTG GTTTTTCTGC 
GAAGAACAGG ATAAGGATGA AAAGACCCCA GTGGACACAG AGGATGTCCA GCCTGCCCCA 
AACATTGATC ATCTGCTCTC CAACATTGGC CGCACAGGCA TGCTGGGTGA GGCTTCAGAC 
TCAACCAACA GTGATTCTGC AAAATCTAAG GGGTCATGGG GATCAAAACG TGACTTTACT 
GCCAAGGATA TCCTTACATT ATCAATTATG TCGGCTGTGA CCCGCAAACG GCGGAAGCGG 
CACAACGGCC GTCTCCTCGG CAGCAGCACA GACGACGATT CTGAGCATGA ACCCATTAAA 
TCCTGCATCT CCGAGAAAGA CGATCTGCAA GAGATGGGGC AGGTTGCATC CTTCTGTGCC 
CCACGAGCAG AGGGAGAGGA GGATGACGAT GAAGAGGAGG AAGATGATGA AGAGGAGGAG 
GAGGGGGCTG AGAGACAGAA GGCACAAGAG CAGAAGGAAA CAGTAGTCCC CAGCATTTCC 
TCAACAGAGG AAGTGACATC AACAGTGCTG CTGAGCGAAG AGGAAGAGCA GAAGTCTGAA 
GTGAAAGGAG GACGCAGCTG GAGATTAGAT GATGCTCGGT CTATTGTGTC AGGCTACTCT 
ACTCTGTCCA CTTTGGGGCG GAGCTTAGCT TCAGAGGGGC GGGGCGAGGA TGCAGATGAT 
GAGCATAGTG AACTAGTGAG TGAAACTGAT AATGAAAGCG GGTTTGCCTC ACGCTCCCTC 
ACCCAAGAAA GGCCAGAAAA ACCAAACCGT TCCAACCAGA ACTCTCATGC GTCTCCTGAA 
CCAACCGCAC CACAGAGTTT CCTCTATGCG CACTGCAAAT CACCTACACC CTCTGGCTCC 
GCCACTGCTC CATCTCCCCC TTCACAACAC ATCACAACCA ACCCAGAGGA CAGCGCAGCA 
CGCTCGTCTA CTCCCTCAAC CTCCTCTTAC TCCTCATCCG CCTCACAACG GCTCCACAAC 
CGCTCTTTCA ACTCCCACCG CCTCATACAG TGCGACACCC TTGCACGCCG TCGGACAAAG 
GCGGACAAAG CCAAAGCTCC CTCAGTGGAC CTTTCCGAAC TCTCGAGCTC CTCCGCCGCA 
GAGGAGGACC GCCAGTCAGG TGCCAATCAA ATCCCTGACT CCTCTCCGTC TCCTTCTTCA 
AAAGCCAAAG GGTTTAGCAT AAGAAACGAG TGTACGCCAA TATCCATGGT TGGTCCCGGA 
AGTAAGGGGT GTGTGTTGTC CCCCGGGCAA GGATCCCTGG CGGATCAGGT ACGTGCTCGT 
CTAATGGGCT CGGCGGATGA CCTGTGCAGC GTTGGCCTGA GAAAGCAGCT CTCGCCGGAG 
ACGCGCCGGA AGAGACGCGC TTGGAGGAGA CACACAGTGG TCGTGTCCCC TACGGATTGT 
TCAGACAAAA AGCCAACGGT CCAAAGCACC AAACCTCTAG CTTCACCCTC CCCTGTTGAA 
AAGCAGGATG TCAGAGAGTC CCACCCAGAG TGCTCAGAAA CTGAAGCCCC TACTGCACGT 
CGGGCCGTGC CTACCTCGCG CTTTCGTGAT TTCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164231.1
CDS	1017..6395
Translation	MWSAGQVPLAMPVVCPHVGGKEWCFQDAVGVDCSSPEPRCIWVAVLHGDTQQINISNLQNDSSPNINGISAGKALSRPAQTSRAKGRRDGLSSSSENPRPVVRSGTDGLGVSWQGPRTLVLQKNSQGFGFTLRHFIVYPPESALHTTLKDEENGNGKGLQRSRLEPMDTIFVKNVREKGPAHLAGLCTGDRLVKVNGESVLGKTYSQVIALIQNSESVLELSIMPKDEDVLQLAYSHDAYLRGNNPYTGKAQNLPPPPPVLYPPRTKSQSPAGFSPCPPPASSHMGQNQLDNWSRWPGSSAGPSPPLDNRTVASSNVVWAAEGRNCEPGGVGHSSPAHRTEEIQYGMTDRGQQPTGQMRGRSYSSSSSSGGMMSPLHHGHLANHNMGTAMGHTGGRKGSPPWASPSQPTSTPSSSRNERTQQALSNWYYSQVPGRERERERERERSSSRSMHPRHRSFSQDRLGELGKARRSQQAGFPHSASQDTLLLLQQSAAAYADPYWNHGDWRGEHMYGQHPSVVAYGRKSSENLLASRYGHSGRSLENLDRAVGILSPRVERSAWVQQPPNRTEVYARHAGHFGQHIESPHQMHHTPQHHQNLTHATHQHSSQIPQPQQAAPMARRLPSCQSLDEEPIGYRSYSPSFNRKTGRILQQAQSFRDPSYTGPHLWTPTSKTSPPEGHPPSATASPAPPTTSSSTTISTPSEGQDRTYRPTNHDRGAGEATSEAQTQEVVLRQKPPTGRRGANALRHPHYAIALDSSDPLLFTTDPTEPTAKAESAISHRRTDVNLPPLSIEDDSLASIPFIGDPKSNRRSSYLLAITTERSKSCDEGLNAYREEGRVFSRLPKRVKSFFTEGSLDSLGAAEEARSKRHSTSELGNISYSDVKREGWLHFKQIHTEKGKKVGSAIWPWRRVFSVLRTHSLFLYKDKREAVLKGAQLGGGAEDEQPISIRGCLVDIAYSETKRKHALRLTTQDFCEYLLQAEDREDMLEWIRVIRDNSKTDNEELGFSRQALINKKLNDYRKQSPTGNKPDSSPRVHRMTFLLSKPDNSAPPRSPKHEAKDESSPPKSPWGINIMKKSKKAGPKAFGVRLEDCQPAVNNKFIPQIVEICCGLVEDMGLEYTGIYRVPGNNAVVSSLQEQLNKGVDINITEEKWQDLNVVSSLLKSFFRKLPEPLFTDDKYNDFIDANRMENASDRLRTMKKLIRDLPDHYFHTLKFLVGHLKTVADHSEKNKMEPRNLALVFGPTLVRTSEDNMTDMVTHMPDRYKIVETLIQHYTWFFCEEQDKDEKTPVDTEDVQPAPNIDHLLSNIGRTGMLGEASDSTNSDSAKSKGSWGSKRDFTAKDILTLSIMSAVTRKRRKRHNGRLLGSSTDDDSEHEPIKSCISEKDDLQEMGQVASFCAPRAEGEEDDDEEEEDDEEEEEGAERQKAQEQKETVVPSISSTEEVTSTVLLSEEEEQKSEVKGGRSWRLDDARSIVSGYSTLSTLGRSLASEGRGEDADDEHSELVSETDNESGFASRSLTQERPEKPNRSNQNSHASPEPTAPQSFLYAHCKSPTPSGSATAPSPPSQHITTNPEDSAARSSTPSTSSYSSSASQRLHNRSFNSHRLIQCDTLARRRTKADKAKAPSVDLSELSSSSAAEEDRQSGANQIPDSSPSPSSKAKGFSIRNECTPISMVGPGSKGCVLSPGQGSLADQVRARLMGSADDLCSVGLRKQLSPETRRKRRAWRRHTVVVSPTDCSDKKPTVQSTKPLASPSPVEKQDVRESHPECSETEAPTARRAVPTSRFRDFL

Target ORF information:

RefSeq Version	XM_005164174.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Rho GTPase activating protein 23a (arhgap23a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164174.3

ORF Insert Sequence:

ATGTGGTCAG CAGGTCAAGT GCCACTGGCG ATGCCCGTTG TGTGCCCGCA TGTAGGGGGT 
AAGGAGTGGT GCTTTCAGGA TGCAGTCGGG GTGGATTGCA GCTCTCCAGA GCCTCGCTGT 
ATCTGGGTGG CGGTTTTACA CGGGGATACA CAGCAAATTA ACATTTCCAA CCTCCAAAAT 
GACTCTTCTC CAAACATAAA TGGCATCAGT GCTGGGAAAG CACTGTCAAG ACCCGCACAG 
ACCTCCAGGG CAAAAGGGCG GAGAGATGGG CTCTCTTCAT CCAGTGAGAA CCCTCGGCCG 
GTGGTCCGGA GCGGGACAGA TGGGCTGGGC GTGTCCTGGC AGGGTCCACG CACGCTGGTG 
CTCCAAAAGA ACTCTCAGGG CTTCGGCTTT ACTCTGCGTC ACTTCATCGT TTACCCGCCA 
GAGTCCGCTC TGCACACCAC TCTCAAGGAT GAAGAGAACG GGAATGGAAA GGGGCTTCAA 
CGAAGTCGTC TGGAGCCCAT GGACACAATT TTTGTGAAGA ATGTGAGGGA AAAAGGCCCC 
GCTCACCTGG CTGGCCTGTG TACAGGAGAC AGGCTGGTTA AAGTGAATGG GGAGAGCGTG 
CTGGGTAAGA CGTACTCGCA GGTCATTGCA CTGATTCAGA ACAGTGAGAG TGTACTGGAG 
CTCTCCATTA TGCCAAAGGA TGAGGACGTG CTTCAGCTGG CATATTCCCA TGATGCCTAC 
CTAAGGGGGA ACAATCCATA TACAGGAAAG GCCCAAAATC TTCCTCCACC ACCCCCTGTT 
CTCTACCCTC CTCGTACAAA ATCACAGTCT CCTGCAGGGT TCAGCCCCTG CCCTCCTCCA 
GCTTCTTCAC ACATGGGGCA AAACCAGCTG GACAACTGGA GCCGCTGGCC CGGCTCTTCT 
GCTGGTCCCT CTCCACCCCT TGACAACCGG ACTGTGGCCT CCAGCAATGT TGTCTGGGCA 
GCCGAGGGCA GGAATTGTGA GCCGGGTGGT GTTGGTCACA GTAGCCCTGC ACACCGAACA 
GAGGAGATCC AGTATGGAAT GACTGACAGA GGACAGCAGC CAACTGGACA AATGAGAGGA 
CGCTCATACT CATCTTCTTC CTCCTCAGGA GGAATGATGA GCCCTTTGCA TCATGGTCAT 
CTTGCCAATC ACAACATGGG TACTGCCATG GGGCACACTG GAGGTCGTAA GGGAAGCCCA 
CCTTGGGCCA GCCCTTCTCA GCCAACATCA ACACCTTCAT CCAGCCGCAA CGAACGAACC 
CAACAAGCCC TTTCCAACTG GTACTACAGC CAGGTCCCAG GCCGTGAGCG AGAACGAGAA 
CGAGAGCGGG AGCGATCTAG TTCTCGCTCA ATGCACCCAC GCCACAGGAG TTTCTCACAA 
GACCGTCTGG GAGAGCTTGG GAAGGCCAGG CGTTCTCAAC AAGCAGGTTT TCCCCATAGT 
GCATCGCAAG ACACGTTGCT GCTGCTTCAG CAGAGTGCTG CAGCATATGC TGATCCTTAC 
TGGAACCATG GAGACTGGCG GGGTGAGCAT ATGTATGGTC AGCACCCATC TGTGGTGGCC 
TATGGGCGAA AGTCCTCCGA AAATCTTCTA GCAAGCCGGT ACGGACACTC GGGACGCTCA 
CTGGAAAATC TCGACAGAGC TGTGGGGATA CTCTCACCAA GGGTTGAAAG ATCTGCTTGG 
GTTCAACAAC CTCCAAATAG GACTGAAGTC TATGCAAGAC ATGCTGGGCA TTTCGGCCAA 
CATATAGAGT CACCCCACCA AATGCACCAC ACACCCCAAC ACCACCAGAA TCTCACACAT 
GCAACACACC AACACTCATC TCAAATCCCA CAACCTCAGC AGGCAGCTCC AATGGCAAGA 
CGTTTGCCTT CTTGCCAGAG TCTAGATGAG GAGCCGATTG GCTATCGTAG CTACAGTCCC 
TCATTCAATA GGAAGACTGG CCGTATCTTG CAGCAGGCCC AGTCATTTAG AGATCCCTCT 
TACACAGGTC CACACCTTTG GACTCCAACA TCCAAAACCA GTCCCCCAGA GGGGCATCCA 
CCCTCAGCCA CTGCTTCCCC AGCCCCACCA ACCACTTCCT CTTCAACTAC GATTTCCACA 
CCATCTGAAG GACAAGACAG AACTTACCGA CCCACTAATC ATGACAGAGG GGCAGGGGAA 
GCGACAAGTG AAGCCCAGAC ACAGGAAGTG GTTCTGAGGC AGAAACCACC TACCGGCCGA 
CGAGGTGCAA ACGCCCTTCG ACACCCACAT TATGCAATAG CACTTGACTC CAGTGACCCT 
CTACTCTTTA CCACTGACCC TACGGAGCCC ACTGCCAAGG CAGAAAGTGC TATTTCTCAT 
CGCCGCACAG ATGTTAACCT TCCTCCACTA TCTATTGAGG ATGACTCCCT TGCATCAATT 
CCATTCATCG GTGATCCTAA ATCTAATCGT CGCTCCTCGT ACCTGTTGGC CATCACCACT 
GAACGCTCCA AGTCATGTGA TGAGGGGCTT AACGCATATA GAGAAGAGGG CCGGGTCTTC 
TCGAGGCTTC CAAAAAGAGT CAAGAGCTTC TTTACTGAAG GGTCTCTGGA CAGTCTGGGT 
GCTGCTGAAG AGGCTCGCTC TAAACGACAC TCTACCTCTG AGCTGGGCAA CATCAGCTAC 
AGTGACGTGA AGAGAGAAGG CTGGCTCCAC TTCAAACAGA TCCACACGGA GAAGGGCAAG 
AAGGTTGGCA GTGCAATCTG GCCATGGAGA CGGGTGTTCT CTGTGCTTAG GACCCATTCT 
CTGTTTCTTT ATAAAGACAA GCGAGAGGCA GTGCTTAAAG GGGCGCAATT AGGGGGCGGG 
GCTGAAGACG AGCAGCCAAT CAGCATCCGG GGCTGCCTTG TGGATATAGC ATACAGTGAA 
ACGAAGCGCA AGCACGCGCT GAGATTGACC ACGCAGGACT TCTGCGAGTA CCTGCTGCAG 
GCGGAGGACA GAGAGGACAT GCTGGAGTGG ATCCGAGTCA TCAGGGACAA CAGCAAGACC 
GACAACGAGG AATTGGGTTT CTCACGACAA GCTCTCATCA ATAAAAAGCT TAACGACTAC 
AGGAAGCAAA GTCCAACGGG TAACAAACCA GACTCCTCAC CCAGAGTGCA TCGAATGACC 
TTCCTCCTCA GCAAGCCAGA CAACAGCGCA CCTCCACGCT CCCCAAAACA CGAGGCCAAA 
GATGAGAGCA GCCCCCCAAA ATCTCCCTGG GGTATAAACA TAATGAAGAA GAGCAAGAAG 
GCCGGTCCTA AAGCTTTTGG CGTCCGTCTA GAGGACTGCC AGCCTGCAGT AAACAACAAG 
TTCATTCCTC AGATTGTGGA GATCTGCTGT GGGCTGGTGG AGGACATGGG TCTGGAGTAT 
ACAGGGATCT ACAGAGTGCC AGGAAACAAT GCTGTAGTCT CCAGTCTGCA GGAGCAGCTC 
AACAAAGGAG TAGACATTAA CATCACTGAG GAGAAATGGC AGGACCTGAA TGTGGTGAGC 
AGCCTGCTGA AGTCCTTCTT TCGTAAACTC CCTGAACCCC TCTTCACTGA TGATAAATAT 
AATGACTTTA TCGACGCCAA TCGGATGGAG AACGCCAGCG ATAGGCTGAG GACAATGAAG 
AAATTGATTC GGGACCTACC AGATCATTAC TTTCACACTC TGAAATTTTT AGTTGGACAC 
CTAAAGACGG TGGCTGACCA CTCTGAGAAA AACAAAATGG AGCCGCGTAA TTTGGCTCTG 
GTGTTTGGCC CCACTCTTGT TCGAACTTCA GAGGACAACA TGACAGACAT GGTCACCCAC 
ATGCCAGACC GCTACAAGAT TGTGGAGACA CTCATTCAGC ATTACACCTG GTTTTTCTGC 
GAAGAACAGG ATAAGGATGA AAAGACCCCA GTGGACACAG AGGATGTCCA GCCTGCCCCA 
AACATTGATC ATCTGCTCTC CAACATTGGC CGCACAGGCA TGCTGGGTGA GGCTTCAGAC 
TCAACCAACA GTGATTCTGC AAAATCTAAG GGGTCATGGG GATCAAAACG TGACTTTACT 
GCCAAGGATA TCCTTACATT ATCAATTATG TCGGCTGTGA CCCGCAAACG GCGGAAGCGG 
CACAACGGCC GTCTCCTCGG CAGCAGCACA GACGACGATT CTGAGCATGA ACCCATTAAA 
TCCTGCATCT CCGAGAAAGA CGATCTGCAA GAGATGGGGC AGGTTGCATC CTTCTGTGCC 
CCACGAGCAG AGGGAGAGGA GGATGACGAT GAAGAGGAGG AAGATGATGA AGAGGAGGAG 
GAGGGGGCTG AGAGACAGAA GGCACAAGAG CAGAAGGAAA CAGTAGTCCC CAGCATTTCC 
TCAACAGAGG AAGTGACATC AACAGTGCTG CTGAGCGAAG AGGAAGAGCA GAAGTCTGAA 
GTGAAAGGAG GACGCAGCTG GAGATTAGAT GATGCTCGGT CTATTGTGTC AGGCTACTCT 
ACTCTGTCCA CTTTGGGGCG GAGCTTAGCT TCAGAGGGGC GGGGCGAGGA TGCAGATGAT 
GAGCATAGTG AACTAGTGAG TGAAACTGAT AATGAAAGCG GGTTTGCCTC ACGCTCCCTC 
ACCCAAGAAA GGCCAGAAAA ACCAAACCGT TCCAACCAGA ACTCTCATGC GTCTCCTGAA 
CCAACCGCAC CACAGAGTTT CCTCTATGCG CACTGCAAAT CACCTACACC CTCTGGCTCC 
GCCACTGCTC CATCTCCCCC TTCACAACAC ATCACAACCA ACCCAGAGGA CAGCGCAGCA 
CGCTCGTCTA CTCCCTCAAC CTCCTCTTAC TCCTCATCCG CCTCACAACG GCTCCACAAC 
CGCTCTTTCA ACTCCCACCG CCTCATACAG TGCGACACCC TTGCACGCCG TCGGACAAAG 
GCGGACAAAG CCAAAGCTCC CTCAGTGGAC CTTTCCGAAC TCTCGAGCTC CTCCGCCGCA 
GAGGAGGACC GCCAGTCAGG TGCCAATCAA ATCCCTGACT CCTCTCCGTC TCCTTCTTCA 
AAAGCCAAAG GGTTTAGCAT AAGAAACGAG TGTACGCCAA TATCCATGGT TGGTCCCGGA 
AGTAAGGGGT GTGTGTTGTC CCCCGGGCAA GGATCCCTGG CGGATCAGGT ACGTGCTCGT 
CTAATGGGCT CGGCGGATGA CCTGTGCAGC GTTGGCCTGA GAAAGCAGCT CTCGCCGGAG 
ACGCGCCGGA AGAGACGCGC TTGGAGGAGA CACACAGTGG TCGTGTCCCC TACGGATTGT 
TCAGACAAAA AGCCAACGGT CCAAAGCACC AAACCTCTAG CTTCACCCTC CCCTGTTGAA 
AAGCAGGATG TCAGAGAGTC CCACCCAGAG TGCTCAGAAA CTGAAGCCCC TACTGCACGT 
CGGGCCGTGC CTACCTCGCG CTTTCGTGAT TTCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap23a ( XM_005164174.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005164231.1 Danio rerio Rho GTPase activating protein 23a (arhgap23a), transcript variant X2, mRNA.

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CloneID	ODa08641
Clone ID Related Accession (Same CDS sequence)	XM_005164174.3 , XM_005164174.4
Accession Version	XM_005164174.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5379bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	rho GTPase-activating protein 23 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005164174.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCAG CAGGTCAAGT GCCACTGGCG ATGCCCGTTG TGTGCCCGCA TGTAGGGGGT 
AAGGAGTGGT GCTTTCAGGA TGCAGTCGGG GTGGATTGCA GCTCTCCAGA GCCTCGCTGT 
ATCTGGGTGG CGGTTTTACA CGGGGATACA CAGCAAATTA ACATTTCCAA CCTCCAAAAT 
GACTCTTCTC CAAACATAAA TGGCATCAGT GCTGGGAAAG CACTGTCAAG ACCCGCACAG 
ACCTCCAGGG CAAAAGGGCG GAGAGATGGG CTCTCTTCAT CCAGTGAGAA CCCTCGGCCG 
GTGGTCCGGA GCGGGACAGA TGGGCTGGGC GTGTCCTGGC AGGGTCCACG CACGCTGGTG 
CTCCAAAAGA ACTCTCAGGG CTTCGGCTTT ACTCTGCGTC ACTTCATCGT TTACCCGCCA 
GAGTCCGCTC TGCACACCAC TCTCAAGGAT GAAGAGAACG GGAATGGAAA GGGGCTTCAA 
CGAAGTCGTC TGGAGCCCAT GGACACAATT TTTGTGAAGA ATGTGAGGGA AAAAGGCCCC 
GCTCACCTGG CTGGCCTGTG TACAGGAGAC AGGCTGGTTA AAGTGAATGG GGAGAGCGTG 
CTGGGTAAGA CGTACTCGCA GGTCATTGCA CTGATTCAGA ACAGTGAGAG TGTACTGGAG 
CTCTCCATTA TGCCAAAGGA TGAGGACGTG CTTCAGCTGG CATATTCCCA TGATGCCTAC 
CTAAGGGGGA ACAATCCATA TACAGGAAAG GCCCAAAATC TTCCTCCACC ACCCCCTGTT 
CTCTACCCTC CTCGTACAAA ATCACAGTCT CCTGCAGGGT TCAGCCCCTG CCCTCCTCCA 
GCTTCTTCAC ACATGGGGCA AAACCAGCTG GACAACTGGA GCCGCTGGCC CGGCTCTTCT 
GCTGGTCCCT CTCCACCCCT TGACAACCGG ACTGTGGCCT CCAGCAATGT TGTCTGGGCA 
GCCGAGGGCA GGAATTGTGA GCCGGGTGGT GTTGGTCACA GTAGCCCTGC ACACCGAACA 
GAGGAGATCC AGTATGGAAT GACTGACAGA GGACAGCAGC CAACTGGACA AATGAGAGGA 
CGCTCATACT CATCTTCTTC CTCCTCAGGA GGAATGATGA GCCCTTTGCA TCATGGTCAT 
CTTGCCAATC ACAACATGGG TACTGCCATG GGGCACACTG GAGGTCGTAA GGGAAGCCCA 
CCTTGGGCCA GCCCTTCTCA GCCAACATCA ACACCTTCAT CCAGCCGCAA CGAACGAACC 
CAACAAGCCC TTTCCAACTG GTACTACAGC CAGGTCCCAG GCCGTGAGCG AGAACGAGAA 
CGAGAGCGGG AGCGATCTAG TTCTCGCTCA ATGCACCCAC GCCACAGGAG TTTCTCACAA 
GACCGTCTGG GAGAGCTTGG GAAGGCCAGG CGTTCTCAAC AAGCAGGTTT TCCCCATAGT 
GCATCGCAAG ACACGTTGCT GCTGCTTCAG CAGAGTGCTG CAGCATATGC TGATCCTTAC 
TGGAACCATG GAGACTGGCG GGGTGAGCAT ATGTATGGTC AGCACCCATC TGTGGTGGCC 
TATGGGCGAA AGTCCTCCGA AAATCTTCTA GCAAGCCGGT ACGGACACTC GGGACGCTCA 
CTGGAAAATC TCGACAGAGC TGTGGGGATA CTCTCACCAA GGGTTGAAAG ATCTGCTTGG 
GTTCAACAAC CTCCAAATAG GACTGAAGTC TATGCAAGAC ATGCTGGGCA TTTCGGCCAA 
CATATAGAGT CACCCCACCA AATGCACCAC ACACCCCAAC ACCACCAGAA TCTCACACAT 
GCAACACACC AACACTCATC TCAAATCCCA CAACCTCAGC AGGCAGCTCC AATGGCAAGA 
CGTTTGCCTT CTTGCCAGAG TCTAGATGAG GAGCCGATTG GCTATCGTAG CTACAGTCCC 
TCATTCAATA GGAAGACTGG CCGTATCTTG CAGCAGGCCC AGTCATTTAG AGATCCCTCT 
TACACAGGTC CACACCTTTG GACTCCAACA TCCAAAACCA GTCCCCCAGA GGGGCATCCA 
CCCTCAGCCA CTGCTTCCCC AGCCCCACCA ACCACTTCCT CTTCAACTAC GATTTCCACA 
CCATCTGAAG GACAAGACAG AACTTACCGA CCCACTAATC ATGACAGAGG GGCAGGGGAA 
GCGACAAGTG AAGCCCAGAC ACAGGAAGTG GTTCTGAGGC AGAAACCACC TACCGGCCGA 
CGAGGTGCAA ACGCCCTTCG ACACCCACAT TATGCAATAG CACTTGACTC CAGTGACCCT 
CTACTCTTTA CCACTGACCC TACGGAGCCC ACTGCCAAGG CAGAAAGTGC TATTTCTCAT 
CGCCGCACAG ATGTTAACCT TCCTCCACTA TCTATTGAGG ATGACTCCCT TGCATCAATT 
CCATTCATCG GTGATCCTAA ATCTAATCGT CGCTCCTCGT ACCTGTTGGC CATCACCACT 
GAACGCTCCA AGTCATGTGA TGAGGGGCTT AACGCATATA GAGAAGAGGG CCGGGTCTTC 
TCGAGGCTTC CAAAAAGAGT CAAGAGCTTC TTTACTGAAG GGTCTCTGGA CAGTCTGGGT 
GCTGCTGAAG AGGCTCGCTC TAAACGACAC TCTACCTCTG AGCTGGGCAA CATCAGCTAC 
AGTGACGTGA AGAGAGAAGG CTGGCTCCAC TTCAAACAGA TCCACACGGA GAAGGGCAAG 
AAGGTTGGCA GTGCAATCTG GCCATGGAGA CGGGTGTTCT CTGTGCTTAG GACCCATTCT 
CTGTTTCTTT ATAAAGACAA GCGAGAGGCA GTGCTTAAAG GGGCGCAATT AGGGGGCGGG 
GCTGAAGACG AGCAGCCAAT CAGCATCCGG GGCTGCCTTG TGGATATAGC ATACAGTGAA 
ACGAAGCGCA AGCACGCGCT GAGATTGACC ACGCAGGACT TCTGCGAGTA CCTGCTGCAG 
GCGGAGGACA GAGAGGACAT GCTGGAGTGG ATCCGAGTCA TCAGGGACAA CAGCAAGACC 
GACAACGAGG AATTGGGTTT CTCACGACAA GCTCTCATCA ATAAAAAGCT TAACGACTAC 
AGGAAGCAAA GTCCAACGGG TAACAAACCA GACTCCTCAC CCAGAGTGCA TCGAATGACC 
TTCCTCCTCA GCAAGCCAGA CAACAGCGCA CCTCCACGCT CCCCAAAACA CGAGGCCAAA 
GATGAGAGCA GCCCCCCAAA ATCTCCCTGG GGTATAAACA TAATGAAGAA GAGCAAGAAG 
GCCGGTCCTA AAGCTTTTGG CGTCCGTCTA GAGGACTGCC AGCCTGCAGT AAACAACAAG 
TTCATTCCTC AGATTGTGGA GATCTGCTGT GGGCTGGTGG AGGACATGGG TCTGGAGTAT 
ACAGGGATCT ACAGAGTGCC AGGAAACAAT GCTGTAGTCT CCAGTCTGCA GGAGCAGCTC 
AACAAAGGAG TAGACATTAA CATCACTGAG GAGAAATGGC AGGACCTGAA TGTGGTGAGC 
AGCCTGCTGA AGTCCTTCTT TCGTAAACTC CCTGAACCCC TCTTCACTGA TGATAAATAT 
AATGACTTTA TCGACGCCAA TCGGATGGAG AACGCCAGCG ATAGGCTGAG GACAATGAAG 
AAATTGATTC GGGACCTACC AGATCATTAC TTTCACACTC TGAAATTTTT AGTTGGACAC 
CTAAAGACGG TGGCTGACCA CTCTGAGAAA AACAAAATGG AGCCGCGTAA TTTGGCTCTG 
GTGTTTGGCC CCACTCTTGT TCGAACTTCA GAGGACAACA TGACAGACAT GGTCACCCAC 
ATGCCAGACC GCTACAAGAT TGTGGAGACA CTCATTCAGC ATTACACCTG GTTTTTCTGC 
GAAGAACAGG ATAAGGATGA AAAGACCCCA GTGGACACAG AGGATGTCCA GCCTGCCCCA 
AACATTGATC ATCTGCTCTC CAACATTGGC CGCACAGGCA TGCTGGGTGA GGCTTCAGAC 
TCAACCAACA GTGATTCTGC AAAATCTAAG GGGTCATGGG GATCAAAACG TGACTTTACT 
GCCAAGGATA TCCTTACATT ATCAATTATG TCGGCTGTGA CCCGCAAACG GCGGAAGCGG 
CACAACGGCC GTCTCCTCGG CAGCAGCACA GACGACGATT CTGAGCATGA ACCCATTAAA 
TCCTGCATCT CCGAGAAAGA CGATCTGCAA GAGATGGGGC AGGTTGCATC CTTCTGTGCC 
CCACGAGCAG AGGGAGAGGA GGATGACGAT GAAGAGGAGG AAGATGATGA AGAGGAGGAG 
GAGGGGGCTG AGAGACAGAA GGCACAAGAG CAGAAGGAAA CAGTAGTCCC CAGCATTTCC 
TCAACAGAGG AAGTGACATC AACAGTGCTG CTGAGCGAAG AGGAAGAGCA GAAGTCTGAA 
GTGAAAGGAG GACGCAGCTG GAGATTAGAT GATGCTCGGT CTATTGTGTC AGGCTACTCT 
ACTCTGTCCA CTTTGGGGCG GAGCTTAGCT TCAGAGGGGC GGGGCGAGGA TGCAGATGAT 
GAGCATAGTG AACTAGTGAG TGAAACTGAT AATGAAAGCG GGTTTGCCTC ACGCTCCCTC 
ACCCAAGAAA GGCCAGAAAA ACCAAACCGT TCCAACCAGA ACTCTCATGC GTCTCCTGAA 
CCAACCGCAC CACAGAGTTT CCTCTATGCG CACTGCAAAT CACCTACACC CTCTGGCTCC 
GCCACTGCTC CATCTCCCCC TTCACAACAC ATCACAACCA ACCCAGAGGA CAGCGCAGCA 
CGCTCGTCTA CTCCCTCAAC CTCCTCTTAC TCCTCATCCG CCTCACAACG GCTCCACAAC 
CGCTCTTTCA ACTCCCACCG CCTCATACAG TGCGACACCC TTGCACGCCG TCGGACAAAG 
GCGGACAAAG CCAAAGCTCC CTCAGTGGAC CTTTCCGAAC TCTCGAGCTC CTCCGCCGCA 
GAGGAGGACC GCCAGTCAGG TGCCAATCAA ATCCCTGACT CCTCTCCGTC TCCTTCTTCA 
AAAGCCAAAG GGTTTAGCAT AAGAAACGAG TGTACGCCAA TATCCATGGT TGGTCCCGGA 
AGTAAGGGGT GTGTGTTGTC CCCCGGGCAA GGATCCCTGG CGGATCAGGT ACGTGCTCGT 
CTAATGGGCT CGGCGGATGA CCTGTGCAGC GTTGGCCTGA GAAAGCAGCT CTCGCCGGAG 
ACGCGCCGGA AGAGACGCGC TTGGAGGAGA CACACAGTGG TCGTGTCCCC TACGGATTGT 
TCAGACAAAA AGCCAACGGT CCAAAGCACC AAACCTCTAG CTTCACCCTC CCCTGTTGAA 
AAGCAGGATG TCAGAGAGTC CCACCCAGAG TGCTCAGAAA CTGAAGCCCC TACTGCACGT 
CGGGCCGTGC CTACCTCGCG CTTTCGTGAT TTCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164231.1
CDS	988..6366
Translation	MWSAGQVPLAMPVVCPHVGGKEWCFQDAVGVDCSSPEPRCIWVAVLHGDTQQINISNLQNDSSPNINGISAGKALSRPAQTSRAKGRRDGLSSSSENPRPVVRSGTDGLGVSWQGPRTLVLQKNSQGFGFTLRHFIVYPPESALHTTLKDEENGNGKGLQRSRLEPMDTIFVKNVREKGPAHLAGLCTGDRLVKVNGESVLGKTYSQVIALIQNSESVLELSIMPKDEDVLQLAYSHDAYLRGNNPYTGKAQNLPPPPPVLYPPRTKSQSPAGFSPCPPPASSHMGQNQLDNWSRWPGSSAGPSPPLDNRTVASSNVVWAAEGRNCEPGGVGHSSPAHRTEEIQYGMTDRGQQPTGQMRGRSYSSSSSSGGMMSPLHHGHLANHNMGTAMGHTGGRKGSPPWASPSQPTSTPSSSRNERTQQALSNWYYSQVPGRERERERERERSSSRSMHPRHRSFSQDRLGELGKARRSQQAGFPHSASQDTLLLLQQSAAAYADPYWNHGDWRGEHMYGQHPSVVAYGRKSSENLLASRYGHSGRSLENLDRAVGILSPRVERSAWVQQPPNRTEVYARHAGHFGQHIESPHQMHHTPQHHQNLTHATHQHSSQIPQPQQAAPMARRLPSCQSLDEEPIGYRSYSPSFNRKTGRILQQAQSFRDPSYTGPHLWTPTSKTSPPEGHPPSATASPAPPTTSSSTTISTPSEGQDRTYRPTNHDRGAGEATSEAQTQEVVLRQKPPTGRRGANALRHPHYAIALDSSDPLLFTTDPTEPTAKAESAISHRRTDVNLPPLSIEDDSLASIPFIGDPKSNRRSSYLLAITTERSKSCDEGLNAYREEGRVFSRLPKRVKSFFTEGSLDSLGAAEEARSKRHSTSELGNISYSDVKREGWLHFKQIHTEKGKKVGSAIWPWRRVFSVLRTHSLFLYKDKREAVLKGAQLGGGAEDEQPISIRGCLVDIAYSETKRKHALRLTTQDFCEYLLQAEDREDMLEWIRVIRDNSKTDNEELGFSRQALINKKLNDYRKQSPTGNKPDSSPRVHRMTFLLSKPDNSAPPRSPKHEAKDESSPPKSPWGINIMKKSKKAGPKAFGVRLEDCQPAVNNKFIPQIVEICCGLVEDMGLEYTGIYRVPGNNAVVSSLQEQLNKGVDINITEEKWQDLNVVSSLLKSFFRKLPEPLFTDDKYNDFIDANRMENASDRLRTMKKLIRDLPDHYFHTLKFLVGHLKTVADHSEKNKMEPRNLALVFGPTLVRTSEDNMTDMVTHMPDRYKIVETLIQHYTWFFCEEQDKDEKTPVDTEDVQPAPNIDHLLSNIGRTGMLGEASDSTNSDSAKSKGSWGSKRDFTAKDILTLSIMSAVTRKRRKRHNGRLLGSSTDDDSEHEPIKSCISEKDDLQEMGQVASFCAPRAEGEEDDDEEEEDDEEEEEGAERQKAQEQKETVVPSISSTEEVTSTVLLSEEEEQKSEVKGGRSWRLDDARSIVSGYSTLSTLGRSLASEGRGEDADDEHSELVSETDNESGFASRSLTQERPEKPNRSNQNSHASPEPTAPQSFLYAHCKSPTPSGSATAPSPPSQHITTNPEDSAARSSTPSTSSYSSSASQRLHNRSFNSHRLIQCDTLARRRTKADKAKAPSVDLSELSSSSAAEEDRQSGANQIPDSSPSPSSKAKGFSIRNECTPISMVGPGSKGCVLSPGQGSLADQVRARLMGSADDLCSVGLRKQLSPETRRKRRAWRRHTVVVSPTDCSDKKPTVQSTKPLASPSPVEKQDVRESHPECSETEAPTARRAVPTSRFRDFL

Target ORF information:

RefSeq Version	XM_005164174.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Rho GTPase activating protein 23a (arhgap23a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164174.4

ORF Insert Sequence:

ATGTGGTCAG CAGGTCAAGT GCCACTGGCG ATGCCCGTTG TGTGCCCGCA TGTAGGGGGT 
AAGGAGTGGT GCTTTCAGGA TGCAGTCGGG GTGGATTGCA GCTCTCCAGA GCCTCGCTGT 
ATCTGGGTGG CGGTTTTACA CGGGGATACA CAGCAAATTA ACATTTCCAA CCTCCAAAAT 
GACTCTTCTC CAAACATAAA TGGCATCAGT GCTGGGAAAG CACTGTCAAG ACCCGCACAG 
ACCTCCAGGG CAAAAGGGCG GAGAGATGGG CTCTCTTCAT CCAGTGAGAA CCCTCGGCCG 
GTGGTCCGGA GCGGGACAGA TGGGCTGGGC GTGTCCTGGC AGGGTCCACG CACGCTGGTG 
CTCCAAAAGA ACTCTCAGGG CTTCGGCTTT ACTCTGCGTC ACTTCATCGT TTACCCGCCA 
GAGTCCGCTC TGCACACCAC TCTCAAGGAT GAAGAGAACG GGAATGGAAA GGGGCTTCAA 
CGAAGTCGTC TGGAGCCCAT GGACACAATT TTTGTGAAGA ATGTGAGGGA AAAAGGCCCC 
GCTCACCTGG CTGGCCTGTG TACAGGAGAC AGGCTGGTTA AAGTGAATGG GGAGAGCGTG 
CTGGGTAAGA CGTACTCGCA GGTCATTGCA CTGATTCAGA ACAGTGAGAG TGTACTGGAG 
CTCTCCATTA TGCCAAAGGA TGAGGACGTG CTTCAGCTGG CATATTCCCA TGATGCCTAC 
CTAAGGGGGA ACAATCCATA TACAGGAAAG GCCCAAAATC TTCCTCCACC ACCCCCTGTT 
CTCTACCCTC CTCGTACAAA ATCACAGTCT CCTGCAGGGT TCAGCCCCTG CCCTCCTCCA 
GCTTCTTCAC ACATGGGGCA AAACCAGCTG GACAACTGGA GCCGCTGGCC CGGCTCTTCT 
GCTGGTCCCT CTCCACCCCT TGACAACCGG ACTGTGGCCT CCAGCAATGT TGTCTGGGCA 
GCCGAGGGCA GGAATTGTGA GCCGGGTGGT GTTGGTCACA GTAGCCCTGC ACACCGAACA 
GAGGAGATCC AGTATGGAAT GACTGACAGA GGACAGCAGC CAACTGGACA AATGAGAGGA 
CGCTCATACT CATCTTCTTC CTCCTCAGGA GGAATGATGA GCCCTTTGCA TCATGGTCAT 
CTTGCCAATC ACAACATGGG TACTGCCATG GGGCACACTG GAGGTCGTAA GGGAAGCCCA 
CCTTGGGCCA GCCCTTCTCA GCCAACATCA ACACCTTCAT CCAGCCGCAA CGAACGAACC 
CAACAAGCCC TTTCCAACTG GTACTACAGC CAGGTCCCAG GCCGTGAGCG AGAACGAGAA 
CGAGAGCGGG AGCGATCTAG TTCTCGCTCA ATGCACCCAC GCCACAGGAG TTTCTCACAA 
GACCGTCTGG GAGAGCTTGG GAAGGCCAGG CGTTCTCAAC AAGCAGGTTT TCCCCATAGT 
GCATCGCAAG ACACGTTGCT GCTGCTTCAG CAGAGTGCTG CAGCATATGC TGATCCTTAC 
TGGAACCATG GAGACTGGCG GGGTGAGCAT ATGTATGGTC AGCACCCATC TGTGGTGGCC 
TATGGGCGAA AGTCCTCCGA AAATCTTCTA GCAAGCCGGT ACGGACACTC GGGACGCTCA 
CTGGAAAATC TCGACAGAGC TGTGGGGATA CTCTCACCAA GGGTTGAAAG ATCTGCTTGG 
GTTCAACAAC CTCCAAATAG GACTGAAGTC TATGCAAGAC ATGCTGGGCA TTTCGGCCAA 
CATATAGAGT CACCCCACCA AATGCACCAC ACACCCCAAC ACCACCAGAA TCTCACACAT 
GCAACACACC AACACTCATC TCAAATCCCA CAACCTCAGC AGGCAGCTCC AATGGCAAGA 
CGTTTGCCTT CTTGCCAGAG TCTAGATGAG GAGCCGATTG GCTATCGTAG CTACAGTCCC 
TCATTCAATA GGAAGACTGG CCGTATCTTG CAGCAGGCCC AGTCATTTAG AGATCCCTCT 
TACACAGGTC CACACCTTTG GACTCCAACA TCCAAAACCA GTCCCCCAGA GGGGCATCCA 
CCCTCAGCCA CTGCTTCCCC AGCCCCACCA ACCACTTCCT CTTCAACTAC GATTTCCACA 
CCATCTGAAG GACAAGACAG AACTTACCGA CCCACTAATC ATGACAGAGG GGCAGGGGAA 
GCGACAAGTG AAGCCCAGAC ACAGGAAGTG GTTCTGAGGC AGAAACCACC TACCGGCCGA 
CGAGGTGCAA ACGCCCTTCG ACACCCACAT TATGCAATAG CACTTGACTC CAGTGACCCT 
CTACTCTTTA CCACTGACCC TACGGAGCCC ACTGCCAAGG CAGAAAGTGC TATTTCTCAT 
CGCCGCACAG ATGTTAACCT TCCTCCACTA TCTATTGAGG ATGACTCCCT TGCATCAATT 
CCATTCATCG GTGATCCTAA ATCTAATCGT CGCTCCTCGT ACCTGTTGGC CATCACCACT 
GAACGCTCCA AGTCATGTGA TGAGGGGCTT AACGCATATA GAGAAGAGGG CCGGGTCTTC 
TCGAGGCTTC CAAAAAGAGT CAAGAGCTTC TTTACTGAAG GGTCTCTGGA CAGTCTGGGT 
GCTGCTGAAG AGGCTCGCTC TAAACGACAC TCTACCTCTG AGCTGGGCAA CATCAGCTAC 
AGTGACGTGA AGAGAGAAGG CTGGCTCCAC TTCAAACAGA TCCACACGGA GAAGGGCAAG 
AAGGTTGGCA GTGCAATCTG GCCATGGAGA CGGGTGTTCT CTGTGCTTAG GACCCATTCT 
CTGTTTCTTT ATAAAGACAA GCGAGAGGCA GTGCTTAAAG GGGCGCAATT AGGGGGCGGG 
GCTGAAGACG AGCAGCCAAT CAGCATCCGG GGCTGCCTTG TGGATATAGC ATACAGTGAA 
ACGAAGCGCA AGCACGCGCT GAGATTGACC ACGCAGGACT TCTGCGAGTA CCTGCTGCAG 
GCGGAGGACA GAGAGGACAT GCTGGAGTGG ATCCGAGTCA TCAGGGACAA CAGCAAGACC 
GACAACGAGG AATTGGGTTT CTCACGACAA GCTCTCATCA ATAAAAAGCT TAACGACTAC 
AGGAAGCAAA GTCCAACGGG TAACAAACCA GACTCCTCAC CCAGAGTGCA TCGAATGACC 
TTCCTCCTCA GCAAGCCAGA CAACAGCGCA CCTCCACGCT CCCCAAAACA CGAGGCCAAA 
GATGAGAGCA GCCCCCCAAA ATCTCCCTGG GGTATAAACA TAATGAAGAA GAGCAAGAAG 
GCCGGTCCTA AAGCTTTTGG CGTCCGTCTA GAGGACTGCC AGCCTGCAGT AAACAACAAG 
TTCATTCCTC AGATTGTGGA GATCTGCTGT GGGCTGGTGG AGGACATGGG TCTGGAGTAT 
ACAGGGATCT ACAGAGTGCC AGGAAACAAT GCTGTAGTCT CCAGTCTGCA GGAGCAGCTC 
AACAAAGGAG TAGACATTAA CATCACTGAG GAGAAATGGC AGGACCTGAA TGTGGTGAGC 
AGCCTGCTGA AGTCCTTCTT TCGTAAACTC CCTGAACCCC TCTTCACTGA TGATAAATAT 
AATGACTTTA TCGACGCCAA TCGGATGGAG AACGCCAGCG ATAGGCTGAG GACAATGAAG 
AAATTGATTC GGGACCTACC AGATCATTAC TTTCACACTC TGAAATTTTT AGTTGGACAC 
CTAAAGACGG TGGCTGACCA CTCTGAGAAA AACAAAATGG AGCCGCGTAA TTTGGCTCTG 
GTGTTTGGCC CCACTCTTGT TCGAACTTCA GAGGACAACA TGACAGACAT GGTCACCCAC 
ATGCCAGACC GCTACAAGAT TGTGGAGACA CTCATTCAGC ATTACACCTG GTTTTTCTGC 
GAAGAACAGG ATAAGGATGA AAAGACCCCA GTGGACACAG AGGATGTCCA GCCTGCCCCA 
AACATTGATC ATCTGCTCTC CAACATTGGC CGCACAGGCA TGCTGGGTGA GGCTTCAGAC 
TCAACCAACA GTGATTCTGC AAAATCTAAG GGGTCATGGG GATCAAAACG TGACTTTACT 
GCCAAGGATA TCCTTACATT ATCAATTATG TCGGCTGTGA CCCGCAAACG GCGGAAGCGG 
CACAACGGCC GTCTCCTCGG CAGCAGCACA GACGACGATT CTGAGCATGA ACCCATTAAA 
TCCTGCATCT CCGAGAAAGA CGATCTGCAA GAGATGGGGC AGGTTGCATC CTTCTGTGCC 
CCACGAGCAG AGGGAGAGGA GGATGACGAT GAAGAGGAGG AAGATGATGA AGAGGAGGAG 
GAGGGGGCTG AGAGACAGAA GGCACAAGAG CAGAAGGAAA CAGTAGTCCC CAGCATTTCC 
TCAACAGAGG AAGTGACATC AACAGTGCTG CTGAGCGAAG AGGAAGAGCA GAAGTCTGAA 
GTGAAAGGAG GACGCAGCTG GAGATTAGAT GATGCTCGGT CTATTGTGTC AGGCTACTCT 
ACTCTGTCCA CTTTGGGGCG GAGCTTAGCT TCAGAGGGGC GGGGCGAGGA TGCAGATGAT 
GAGCATAGTG AACTAGTGAG TGAAACTGAT AATGAAAGCG GGTTTGCCTC ACGCTCCCTC 
ACCCAAGAAA GGCCAGAAAA ACCAAACCGT TCCAACCAGA ACTCTCATGC GTCTCCTGAA 
CCAACCGCAC CACAGAGTTT CCTCTATGCG CACTGCAAAT CACCTACACC CTCTGGCTCC 
GCCACTGCTC CATCTCCCCC TTCACAACAC ATCACAACCA ACCCAGAGGA CAGCGCAGCA 
CGCTCGTCTA CTCCCTCAAC CTCCTCTTAC TCCTCATCCG CCTCACAACG GCTCCACAAC 
CGCTCTTTCA ACTCCCACCG CCTCATACAG TGCGACACCC TTGCACGCCG TCGGACAAAG 
GCGGACAAAG CCAAAGCTCC CTCAGTGGAC CTTTCCGAAC TCTCGAGCTC CTCCGCCGCA 
GAGGAGGACC GCCAGTCAGG TGCCAATCAA ATCCCTGACT CCTCTCCGTC TCCTTCTTCA 
AAAGCCAAAG GGTTTAGCAT AAGAAACGAG TGTACGCCAA TATCCATGGT TGGTCCCGGA 
AGTAAGGGGT GTGTGTTGTC CCCCGGGCAA GGATCCCTGG CGGATCAGGT ACGTGCTCGT 
CTAATGGGCT CGGCGGATGA CCTGTGCAGC GTTGGCCTGA GAAAGCAGCT CTCGCCGGAG 
ACGCGCCGGA AGAGACGCGC TTGGAGGAGA CACACAGTGG TCGTGTCCCC TACGGATTGT 
TCAGACAAAA AGCCAACGGT CCAAAGCACC AAACCTCTAG CTTCACCCTC CCCTGTTGAA 
AAGCAGGATG TCAGAGAGTC CCACCCAGAG TGCTCAGAAA CTGAAGCCCC TACTGCACGT 
CGGGCCGTGC CTACCTCGCG CTTTCGTGAT TTCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap23a ( XM_005164175.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005164232.1 Danio rerio Rho GTPase activating protein 23a (arhgap23a), transcript variant X5, mRNA.

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CloneID	ODa08642
Clone ID Related Accession (Same CDS sequence)	XM_005164175.3 , XM_005164175.4
Accession Version	XM_005164175.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	rho GTPase-activating protein 23 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005164175.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA ACCAGCTGGA CAACTGGAGC CGCTGGCCCG GCTCTTCTGC TGGTCCCTCT 
CCACCCCTTG ACAACCGGAC TGTGGCCTCC AGCAATGTTG TCTGGGCAGC CGAGGGCAGG 
AATTGTGAGC CGGGTGGTGT TGGTCACAGT AGCCCTGCAC ACCGAACAGA GGAGATCCAG 
TATGGAATGA CTGACAGAGG ACAGCAGCCA ACTGGACAAA TGAGAGGACG CTCATACTCA 
TCTTCTTCCT CCTCAGGAGG AATGATGAGC CCTTTGCATC ATGGTCATCT TGCCAATCAC 
AACATGGGTA CTGCCATGGG GCACACTGGA GGTCGTAAGG GAAGCCCACC TTGGGCCAGC 
CCTTCTCAGC CAACATCAAC ACCTTCATCC AGCCGCAACG AACGAACCCA ACAAGCCCTT 
TCCAACTGGT ACTACAGCCA GGTCCCAGGC CGTGAGCGAG AACGAGAACG AGAGCGGGAG 
CGATCTAGTT CTCGCTCAAT GCACCCACGC CACAGGAGTT TCTCACAAGA CCGTCTGGGA 
GAGCTTGGGA AGGCCAGGCG TTCTCAACAA GCAGGTTTTC CCCATAGTGC ATCGCAAGAC 
ACGTTGCTGC TGCTTCAGCA GAGTGCTGCA GCATATGCTG ATCCTTACTG GAACCATGGA 
GACTGGCGGG GTGAGCATAT GTATGGTCAG CACCCATCTG TGGTGGCCTA TGGGCGAAAG 
TCCTCCGAAA ATCTTCTAGC AAGCCGGTAC GGACACTCGG GACGCTCACT GGAAAATCTC 
GACAGAGCTG TGGGGATACT CTCACCAAGG GTTGAAAGAT CTGCTTGGGT TCAACAACCT 
CCAAATAGGA CTGAAGTCTA TGCAAGACAT GCTGGGCATT TCGGCCAACA TATAGAGTCA 
CCCCACCAAA TGCACCACAC ACCCCAACAC CACCAGAATC TCACACATGC AACACACCAA 
CACTCATCTC AAATCCCACA ACCTCAGCAG GCAGCTCCAA TGGCAAGACG TTTGCCTTCT 
TGCCAGAGTC TAGATGAGGA GCCGATTGGC TATCGTAGCT ACAGTCCCTC ATTCAATAGG 
AAGACTGGCC GTATCTTGCA GCAGGCCCAG TCATTTAGAG ATCCCTCTTA CACAGGTCCA 
CACCTTTGGA CTCCAACATC CAAAACCAGT CCCCCAGAGG GGCATCCACC CTCAGCCACT 
GCTTCCCCAG CCCCACCAAC CACTTCCTCT TCAACTACGA TTTCCACACC ATCTGAAGGA 
CAAGACAGAA CTTACCGACC CACTAATCAT GACAGAGGGG CAGGGGAAGC GACAAGTGAA 
GCCCAGACAC AGGAAGTGGT TCTGAGGCAG AAACCACCTA CCGGCCGACG AGGTGCAAAC 
GCCCTTCGAC ACCCACATTA TGCAATAGCA CTTGACTCCA GTGACCCTCT ACTCTTTACC 
ACTGACCCTA CGGAGCCCAC TGCCAAGGCA GAAAGTGCTA TTTCTCATCG CCGCACAGAT 
GTTAACCTTC CTCCACTATC TATTGAGGAT GACTCCCTTG CATCAATTCC ATTCATCGAT 
GAGCCGACCA GTCCAAGTGC AGATCTGCGG GCACGTCATG TCCCCGCCTC CTCCGTCGTG 
TCCAGTGGCG GTATAAACTC CGCCCCTGCT GTGATCACAA GCCCCGCCTC CCCAACCTTC 
ACCTTTCCCC TCAACCGCCT TTTCTCCCAC AACTGCAGTG ATCCTAAATC TAATCGTCGC 
TCCTCGTACC TGTTGGCCAT CACCACTGAA CGCTCCAAGT CATGTGATGA GGGGCTTAAC 
GCATATAGAG AAGAGGGCCG GGTCTTCTCG AGGCTTCCAA AAAGAGTCAA GAGCTTCTTT 
ACTGAAGGGT CTCTGGACAG TCTGGGTGCT GCTGAAGAGG CTCGCTCTAA ACGACACTCT 
ACCTCTGAGC TGGGCAACAT CAGCTACAGT GACGTGAAGA GAGAAGGCTG GCTCCACTTC 
AAACAGATCC ACACGGAGAA GGGCAAGAAG GTTGGCAGTG CAATCTGGCC ATGGAGACGG 
GTGTTCTCTG TGCTTAGGAC CCATTCTCTG TTTCTTTATA AAGACAAGCG AGAGGCAGTG 
CTTAAAGGGG CGCAATTAGG GGGCGGGGCT GAAGACGAGC AGCCAATCAG CATCCGGGGC 
TGCCTTGTGG ATATAGCATA CAGTGAAACG AAGCGCAAGC ACGCGCTGAG ATTGACCACG 
CAGGACTTCT GCGAGTACCT GCTGCAGGCG GAGGACAGAG AGGACATGCT GGAGTGGATC 
CGAGTCATCA GGGACAACAG CAAGACCGAC AACGAGGAAT TGGGTTTCTC ACGACAAGCT 
CTCATCAATA AAAAGCTTAA CGACTACAGG AAGCAAAGTC CAACGGGTAA CAAACCAGAC 
TCCTCACCCA GAGTGCATCG AATGACCTTC CTCCTCAGCA AGCCAGACAA CAGCGCACCT 
CCACGCTCCC CAAAACACGA GGCCAAAGAT GAGAGCAGCC CCCCAAAATC TCCCTGGGGT 
ATAAACATAA TGAAGAAGAG CAAGAAGGCC GGTCCTAAAG CTTTTGGCGT CCGTCTAGAG 
GACTGCCAGC CTGCAGTAAA CAACAAGTTC ATTCCTCAGA TTGTGGAGAT CTGCTGTGGG 
CTGGTGGAGG ACATGGGTCT GGAGTATACA GGGATCTACA GAGTGCCAGG AAACAATGCT 
GTAGTCTCCA GTCTGCAGGA GCAGCTCAAC AAAGGAGTAG ACATTAACAT CACTGAGGAG 
AAATGGCAGG ACCTGAATGT GGTGAGCAGC CTGCTGAAGT CCTTCTTTCG TAAACTCCCT 
GAACCCCTCT TCACTGATGA TAAATATAAT GACTTTATCG ACGCCAATCG GATGGAGAAC 
GCCAGCGATA GGCTGAGGAC AATGAAGAAA TTGATTCGGG ACCTACCAGA TCATTACTTT 
CACACTCTGA AATTTTTAGT TGGACACCTA AAGACGGTGG CTGACCACTC TGAGAAAAAC 
AAAATGGAGC CGCGTAATTT GGCTCTGGTG TTTGGCCCCA CTCTTGTTCG AACTTCAGAG 
GACAACATGA CAGACATGGT CACCCACATG CCAGACCGCT ACAAGATTGT GGAGACACTC 
ATTCAGCATT ACACCTGGTT TTTCTGCGAA GAACAGGATA AGGATGAAAA GACCCCAGTG 
GACACAGAGG ATGTCCAGCC TGCCCCAAAC ATTGATCATC TGCTCTCCAA CATTGGCCGC 
ACAGGCATGC TGGGTGAGGC TTCAGACTCA ACCAACAGTG ATTCTGCAAA ATCTAAGGGG 
TCATGGGGAT CAAAACGTGA CTTTACTGCC AAGGATATCC TTACATTATC AATTATGTCG 
GCTGTGACCC GCAAACGGCG GAAGCGGCAC AACGGCCGTC TCCTCGGCAG CAGCACAGAC 
GACGATTCTG AGCATGAACC CATTAAATCC TGCATCTCCG AGAAAGACGA TCTGCAAGAG 
ATGGGGCAGG TTGCATCCTT CTGTGCCCCA CGAGCAGAGG GAGAGGAGGA TGACGATGAA 
GAGGAGGAAG ATGATGAAGA GGAGGAGGAG GGGGCTGAGA GACAGAAGGC ACAAGAGCAG 
AAGGAAACAG TAGTCCCCAG CATTTCCTCA ACAGAGGAAG TGACATCAAC AGTGCTGCTG 
AGCGAAGAGG AAGAGCAGAA GTCTGAAGTG AAAGGAGGAC GCAGCTGGAG ATTAGATGAT 
GCTCGGTCTA TTGTGTCAGG CTACTCTACT CTGTCCACTT TGGGGCGGAG CTTAGCTTCA 
GAGGGGCGGG GCGAGGATGC AGATGATGAG CATAGTGAAC TAGTGAGTGA AACTGATAAT 
GAAAGCGGGT TTGCCTCACG CTCCCTCACC CAAGAAAGGC CAGAAAAACC AAACCGTTCC 
AACCAGAACT CTCATGCGTC TCCTGAACCA ACCGCACCAC AGAGTTTCCT CTATGCGCAC 
TGCAAATCAC CTACACCCTC TGGCTCCGCC ACTGCTCCAT CTCCCCCTTC ACAACACATC 
ACAACCAACC CAGAGGACAG CGCAGCACGC TCGTCTACTC CCTCAACCTC CTCTTACTCC 
TCATCCGCCT CACAACGGCT CCACAACCGC TCTTTCAACT CCCACCGCCT CATACAGTGC 
GACACCCTTG CACGCCGTCG GACAAAGGCG GACAAAGCCA AAGCTCCCTC AGTGGACCTT 
TCCGAACTCT CGAGCTCCTC CGCCGCAGAG GAGGACCGCC AGTCAGGTGC CAATCAAATC 
CCTGACTCCT CTCCGTCTCC TTCTTCAAAA GCCAAAGGGT TTAGCATAAG AAACGAGTGT 
ACGCCAATAT CCATGGTTGG TCCCGGAAGT AAGGGGTGTG TGTTGTCCCC CGGGCAAGGA 
TCCCTGGCGG ATCAGGTACG TGCTCGTCTA ATGGGCTCGG CGGATGACCT GTGCAGCGTT 
GGCCTGAGAA AGCAGCTCTC GCCGGAGACG CGCCGGAAGA GACGCGCTTG GAGGAGACAC 
ACAGTGGTCG TGTCCCCTAC GGATTGTTCA GACAAAAAGC CAACGGTCCA AAGCACCAAA 
CCTCTAGCTT CACCCTCCCC TGTTGAAAAG CAGGATGTCA GAGAGTCCCA CCCAGAGTGC 
TCAGAAACTG AAGCCCCTAC TGCACGTCGG GCCGTGCCTA CCTCGCGCTT TCGTGATTTC 
TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164232.1
CDS	474..5159
Translation	MGQNQLDNWSRWPGSSAGPSPPLDNRTVASSNVVWAAEGRNCEPGGVGHSSPAHRTEEIQYGMTDRGQQPTGQMRGRSYSSSSSSGGMMSPLHHGHLANHNMGTAMGHTGGRKGSPPWASPSQPTSTPSSSRNERTQQALSNWYYSQVPGRERERERERERSSSRSMHPRHRSFSQDRLGELGKARRSQQAGFPHSASQDTLLLLQQSAAAYADPYWNHGDWRGEHMYGQHPSVVAYGRKSSENLLASRYGHSGRSLENLDRAVGILSPRVERSAWVQQPPNRTEVYARHAGHFGQHIESPHQMHHTPQHHQNLTHATHQHSSQIPQPQQAAPMARRLPSCQSLDEEPIGYRSYSPSFNRKTGRILQQAQSFRDPSYTGPHLWTPTSKTSPPEGHPPSATASPAPPTTSSSTTISTPSEGQDRTYRPTNHDRGAGEATSEAQTQEVVLRQKPPTGRRGANALRHPHYAIALDSSDPLLFTTDPTEPTAKAESAISHRRTDVNLPPLSIEDDSLASIPFIDEPTSPSADLRARHVPASSVVSSGGINSAPAVITSPASPTFTFPLNRLFSHNCSDPKSNRRSSYLLAITTERSKSCDEGLNAYREEGRVFSRLPKRVKSFFTEGSLDSLGAAEEARSKRHSTSELGNISYSDVKREGWLHFKQIHTEKGKKVGSAIWPWRRVFSVLRTHSLFLYKDKREAVLKGAQLGGGAEDEQPISIRGCLVDIAYSETKRKHALRLTTQDFCEYLLQAEDREDMLEWIRVIRDNSKTDNEELGFSRQALINKKLNDYRKQSPTGNKPDSSPRVHRMTFLLSKPDNSAPPRSPKHEAKDESSPPKSPWGINIMKKSKKAGPKAFGVRLEDCQPAVNNKFIPQIVEICCGLVEDMGLEYTGIYRVPGNNAVVSSLQEQLNKGVDINITEEKWQDLNVVSSLLKSFFRKLPEPLFTDDKYNDFIDANRMENASDRLRTMKKLIRDLPDHYFHTLKFLVGHLKTVADHSEKNKMEPRNLALVFGPTLVRTSEDNMTDMVTHMPDRYKIVETLIQHYTWFFCEEQDKDEKTPVDTEDVQPAPNIDHLLSNIGRTGMLGEASDSTNSDSAKSKGSWGSKRDFTAKDILTLSIMSAVTRKRRKRHNGRLLGSSTDDDSEHEPIKSCISEKDDLQEMGQVASFCAPRAEGEEDDDEEEEDDEEEEEGAERQKAQEQKETVVPSISSTEEVTSTVLLSEEEEQKSEVKGGRSWRLDDARSIVSGYSTLSTLGRSLASEGRGEDADDEHSELVSETDNESGFASRSLTQERPEKPNRSNQNSHASPEPTAPQSFLYAHCKSPTPSGSATAPSPPSQHITTNPEDSAARSSTPSTSSYSSSASQRLHNRSFNSHRLIQCDTLARRRTKADKAKAPSVDLSELSSSSAAEEDRQSGANQIPDSSPSPSSKAKGFSIRNECTPISMVGPGSKGCVLSPGQGSLADQVRARLMGSADDLCSVGLRKQLSPETRRKRRAWRRHTVVVSPTDCSDKKPTVQSTKPLASPSPVEKQDVRESHPECSETEAPTARRAVPTSRFRDFL

Target ORF information:

RefSeq Version	XM_005164175.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Rho GTPase activating protein 23a (arhgap23a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164175.3

ORF Insert Sequence:

ATGGGGCAAA ACCAGCTGGA CAACTGGAGC CGCTGGCCCG GCTCTTCTGC TGGTCCCTCT 
CCACCCCTTG ACAACCGGAC TGTGGCCTCC AGCAATGTTG TCTGGGCAGC CGAGGGCAGG 
AATTGTGAGC CGGGTGGTGT TGGTCACAGT AGCCCTGCAC ACCGAACAGA GGAGATCCAG 
TATGGAATGA CTGACAGAGG ACAGCAGCCA ACTGGACAAA TGAGAGGACG CTCATACTCA 
TCTTCTTCCT CCTCAGGAGG AATGATGAGC CCTTTGCATC ATGGTCATCT TGCCAATCAC 
AACATGGGTA CTGCCATGGG GCACACTGGA GGTCGTAAGG GAAGCCCACC TTGGGCCAGC 
CCTTCTCAGC CAACATCAAC ACCTTCATCC AGCCGCAACG AACGAACCCA ACAAGCCCTT 
TCCAACTGGT ACTACAGCCA GGTCCCAGGC CGTGAGCGAG AACGAGAACG AGAGCGGGAG 
CGATCTAGTT CTCGCTCAAT GCACCCACGC CACAGGAGTT TCTCACAAGA CCGTCTGGGA 
GAGCTTGGGA AGGCCAGGCG TTCTCAACAA GCAGGTTTTC CCCATAGTGC ATCGCAAGAC 
ACGTTGCTGC TGCTTCAGCA GAGTGCTGCA GCATATGCTG ATCCTTACTG GAACCATGGA 
GACTGGCGGG GTGAGCATAT GTATGGTCAG CACCCATCTG TGGTGGCCTA TGGGCGAAAG 
TCCTCCGAAA ATCTTCTAGC AAGCCGGTAC GGACACTCGG GACGCTCACT GGAAAATCTC 
GACAGAGCTG TGGGGATACT CTCACCAAGG GTTGAAAGAT CTGCTTGGGT TCAACAACCT 
CCAAATAGGA CTGAAGTCTA TGCAAGACAT GCTGGGCATT TCGGCCAACA TATAGAGTCA 
CCCCACCAAA TGCACCACAC ACCCCAACAC CACCAGAATC TCACACATGC AACACACCAA 
CACTCATCTC AAATCCCACA ACCTCAGCAG GCAGCTCCAA TGGCAAGACG TTTGCCTTCT 
TGCCAGAGTC TAGATGAGGA GCCGATTGGC TATCGTAGCT ACAGTCCCTC ATTCAATAGG 
AAGACTGGCC GTATCTTGCA GCAGGCCCAG TCATTTAGAG ATCCCTCTTA CACAGGTCCA 
CACCTTTGGA CTCCAACATC CAAAACCAGT CCCCCAGAGG GGCATCCACC CTCAGCCACT 
GCTTCCCCAG CCCCACCAAC CACTTCCTCT TCAACTACGA TTTCCACACC ATCTGAAGGA 
CAAGACAGAA CTTACCGACC CACTAATCAT GACAGAGGGG CAGGGGAAGC GACAAGTGAA 
GCCCAGACAC AGGAAGTGGT TCTGAGGCAG AAACCACCTA CCGGCCGACG AGGTGCAAAC 
GCCCTTCGAC ACCCACATTA TGCAATAGCA CTTGACTCCA GTGACCCTCT ACTCTTTACC 
ACTGACCCTA CGGAGCCCAC TGCCAAGGCA GAAAGTGCTA TTTCTCATCG CCGCACAGAT 
GTTAACCTTC CTCCACTATC TATTGAGGAT GACTCCCTTG CATCAATTCC ATTCATCGAT 
GAGCCGACCA GTCCAAGTGC AGATCTGCGG GCACGTCATG TCCCCGCCTC CTCCGTCGTG 
TCCAGTGGCG GTATAAACTC CGCCCCTGCT GTGATCACAA GCCCCGCCTC CCCAACCTTC 
ACCTTTCCCC TCAACCGCCT TTTCTCCCAC AACTGCAGTG ATCCTAAATC TAATCGTCGC 
TCCTCGTACC TGTTGGCCAT CACCACTGAA CGCTCCAAGT CATGTGATGA GGGGCTTAAC 
GCATATAGAG AAGAGGGCCG GGTCTTCTCG AGGCTTCCAA AAAGAGTCAA GAGCTTCTTT 
ACTGAAGGGT CTCTGGACAG TCTGGGTGCT GCTGAAGAGG CTCGCTCTAA ACGACACTCT 
ACCTCTGAGC TGGGCAACAT CAGCTACAGT GACGTGAAGA GAGAAGGCTG GCTCCACTTC 
AAACAGATCC ACACGGAGAA GGGCAAGAAG GTTGGCAGTG CAATCTGGCC ATGGAGACGG 
GTGTTCTCTG TGCTTAGGAC CCATTCTCTG TTTCTTTATA AAGACAAGCG AGAGGCAGTG 
CTTAAAGGGG CGCAATTAGG GGGCGGGGCT GAAGACGAGC AGCCAATCAG CATCCGGGGC 
TGCCTTGTGG ATATAGCATA CAGTGAAACG AAGCGCAAGC ACGCGCTGAG ATTGACCACG 
CAGGACTTCT GCGAGTACCT GCTGCAGGCG GAGGACAGAG AGGACATGCT GGAGTGGATC 
CGAGTCATCA GGGACAACAG CAAGACCGAC AACGAGGAAT TGGGTTTCTC ACGACAAGCT 
CTCATCAATA AAAAGCTTAA CGACTACAGG AAGCAAAGTC CAACGGGTAA CAAACCAGAC 
TCCTCACCCA GAGTGCATCG AATGACCTTC CTCCTCAGCA AGCCAGACAA CAGCGCACCT 
CCACGCTCCC CAAAACACGA GGCCAAAGAT GAGAGCAGCC CCCCAAAATC TCCCTGGGGT 
ATAAACATAA TGAAGAAGAG CAAGAAGGCC GGTCCTAAAG CTTTTGGCGT CCGTCTAGAG 
GACTGCCAGC CTGCAGTAAA CAACAAGTTC ATTCCTCAGA TTGTGGAGAT CTGCTGTGGG 
CTGGTGGAGG ACATGGGTCT GGAGTATACA GGGATCTACA GAGTGCCAGG AAACAATGCT 
GTAGTCTCCA GTCTGCAGGA GCAGCTCAAC AAAGGAGTAG ACATTAACAT CACTGAGGAG 
AAATGGCAGG ACCTGAATGT GGTGAGCAGC CTGCTGAAGT CCTTCTTTCG TAAACTCCCT 
GAACCCCTCT TCACTGATGA TAAATATAAT GACTTTATCG ACGCCAATCG GATGGAGAAC 
GCCAGCGATA GGCTGAGGAC AATGAAGAAA TTGATTCGGG ACCTACCAGA TCATTACTTT 
CACACTCTGA AATTTTTAGT TGGACACCTA AAGACGGTGG CTGACCACTC TGAGAAAAAC 
AAAATGGAGC CGCGTAATTT GGCTCTGGTG TTTGGCCCCA CTCTTGTTCG AACTTCAGAG 
GACAACATGA CAGACATGGT CACCCACATG CCAGACCGCT ACAAGATTGT GGAGACACTC 
ATTCAGCATT ACACCTGGTT TTTCTGCGAA GAACAGGATA AGGATGAAAA GACCCCAGTG 
GACACAGAGG ATGTCCAGCC TGCCCCAAAC ATTGATCATC TGCTCTCCAA CATTGGCCGC 
ACAGGCATGC TGGGTGAGGC TTCAGACTCA ACCAACAGTG ATTCTGCAAA ATCTAAGGGG 
TCATGGGGAT CAAAACGTGA CTTTACTGCC AAGGATATCC TTACATTATC AATTATGTCG 
GCTGTGACCC GCAAACGGCG GAAGCGGCAC AACGGCCGTC TCCTCGGCAG CAGCACAGAC 
GACGATTCTG AGCATGAACC CATTAAATCC TGCATCTCCG AGAAAGACGA TCTGCAAGAG 
ATGGGGCAGG TTGCATCCTT CTGTGCCCCA CGAGCAGAGG GAGAGGAGGA TGACGATGAA 
GAGGAGGAAG ATGATGAAGA GGAGGAGGAG GGGGCTGAGA GACAGAAGGC ACAAGAGCAG 
AAGGAAACAG TAGTCCCCAG CATTTCCTCA ACAGAGGAAG TGACATCAAC AGTGCTGCTG 
AGCGAAGAGG AAGAGCAGAA GTCTGAAGTG AAAGGAGGAC GCAGCTGGAG ATTAGATGAT 
GCTCGGTCTA TTGTGTCAGG CTACTCTACT CTGTCCACTT TGGGGCGGAG CTTAGCTTCA 
GAGGGGCGGG GCGAGGATGC AGATGATGAG CATAGTGAAC TAGTGAGTGA AACTGATAAT 
GAAAGCGGGT TTGCCTCACG CTCCCTCACC CAAGAAAGGC CAGAAAAACC AAACCGTTCC 
AACCAGAACT CTCATGCGTC TCCTGAACCA ACCGCACCAC AGAGTTTCCT CTATGCGCAC 
TGCAAATCAC CTACACCCTC TGGCTCCGCC ACTGCTCCAT CTCCCCCTTC ACAACACATC 
ACAACCAACC CAGAGGACAG CGCAGCACGC TCGTCTACTC CCTCAACCTC CTCTTACTCC 
TCATCCGCCT CACAACGGCT CCACAACCGC TCTTTCAACT CCCACCGCCT CATACAGTGC 
GACACCCTTG CACGCCGTCG GACAAAGGCG GACAAAGCCA AAGCTCCCTC AGTGGACCTT 
TCCGAACTCT CGAGCTCCTC CGCCGCAGAG GAGGACCGCC AGTCAGGTGC CAATCAAATC 
CCTGACTCCT CTCCGTCTCC TTCTTCAAAA GCCAAAGGGT TTAGCATAAG AAACGAGTGT 
ACGCCAATAT CCATGGTTGG TCCCGGAAGT AAGGGGTGTG TGTTGTCCCC CGGGCAAGGA 
TCCCTGGCGG ATCAGGTACG TGCTCGTCTA ATGGGCTCGG CGGATGACCT GTGCAGCGTT 
GGCCTGAGAA AGCAGCTCTC GCCGGAGACG CGCCGGAAGA GACGCGCTTG GAGGAGACAC 
ACAGTGGTCG TGTCCCCTAC GGATTGTTCA GACAAAAAGC CAACGGTCCA AAGCACCAAA 
CCTCTAGCTT CACCCTCCCC TGTTGAAAAG CAGGATGTCA GAGAGTCCCA CCCAGAGTGC 
TCAGAAACTG AAGCCCCTAC TGCACGTCGG GCCGTGCCTA CCTCGCGCTT TCGTGATTTC 
TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap23a ( XM_005164175.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005164232.1 Danio rerio Rho GTPase activating protein 23a (arhgap23a), transcript variant X5, mRNA.

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CloneID	ODa08642
Clone ID Related Accession (Same CDS sequence)	XM_005164175.3 , XM_005164175.4
Accession Version	XM_005164175.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	rho GTPase-activating protein 23 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005164175.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCAAA ACCAGCTGGA CAACTGGAGC CGCTGGCCCG GCTCTTCTGC TGGTCCCTCT 
CCACCCCTTG ACAACCGGAC TGTGGCCTCC AGCAATGTTG TCTGGGCAGC CGAGGGCAGG 
AATTGTGAGC CGGGTGGTGT TGGTCACAGT AGCCCTGCAC ACCGAACAGA GGAGATCCAG 
TATGGAATGA CTGACAGAGG ACAGCAGCCA ACTGGACAAA TGAGAGGACG CTCATACTCA 
TCTTCTTCCT CCTCAGGAGG AATGATGAGC CCTTTGCATC ATGGTCATCT TGCCAATCAC 
AACATGGGTA CTGCCATGGG GCACACTGGA GGTCGTAAGG GAAGCCCACC TTGGGCCAGC 
CCTTCTCAGC CAACATCAAC ACCTTCATCC AGCCGCAACG AACGAACCCA ACAAGCCCTT 
TCCAACTGGT ACTACAGCCA GGTCCCAGGC CGTGAGCGAG AACGAGAACG AGAGCGGGAG 
CGATCTAGTT CTCGCTCAAT GCACCCACGC CACAGGAGTT TCTCACAAGA CCGTCTGGGA 
GAGCTTGGGA AGGCCAGGCG TTCTCAACAA GCAGGTTTTC CCCATAGTGC ATCGCAAGAC 
ACGTTGCTGC TGCTTCAGCA GAGTGCTGCA GCATATGCTG ATCCTTACTG GAACCATGGA 
GACTGGCGGG GTGAGCATAT GTATGGTCAG CACCCATCTG TGGTGGCCTA TGGGCGAAAG 
TCCTCCGAAA ATCTTCTAGC AAGCCGGTAC GGACACTCGG GACGCTCACT GGAAAATCTC 
GACAGAGCTG TGGGGATACT CTCACCAAGG GTTGAAAGAT CTGCTTGGGT TCAACAACCT 
CCAAATAGGA CTGAAGTCTA TGCAAGACAT GCTGGGCATT TCGGCCAACA TATAGAGTCA 
CCCCACCAAA TGCACCACAC ACCCCAACAC CACCAGAATC TCACACATGC AACACACCAA 
CACTCATCTC AAATCCCACA ACCTCAGCAG GCAGCTCCAA TGGCAAGACG TTTGCCTTCT 
TGCCAGAGTC TAGATGAGGA GCCGATTGGC TATCGTAGCT ACAGTCCCTC ATTCAATAGG 
AAGACTGGCC GTATCTTGCA GCAGGCCCAG TCATTTAGAG ATCCCTCTTA CACAGGTCCA 
CACCTTTGGA CTCCAACATC CAAAACCAGT CCCCCAGAGG GGCATCCACC CTCAGCCACT 
GCTTCCCCAG CCCCACCAAC CACTTCCTCT TCAACTACGA TTTCCACACC ATCTGAAGGA 
CAAGACAGAA CTTACCGACC CACTAATCAT GACAGAGGGG CAGGGGAAGC GACAAGTGAA 
GCCCAGACAC AGGAAGTGGT TCTGAGGCAG AAACCACCTA CCGGCCGACG AGGTGCAAAC 
GCCCTTCGAC ACCCACATTA TGCAATAGCA CTTGACTCCA GTGACCCTCT ACTCTTTACC 
ACTGACCCTA CGGAGCCCAC TGCCAAGGCA GAAAGTGCTA TTTCTCATCG CCGCACAGAT 
GTTAACCTTC CTCCACTATC TATTGAGGAT GACTCCCTTG CATCAATTCC ATTCATCGAT 
GAGCCGACCA GTCCAAGTGC AGATCTGCGG GCACGTCATG TCCCCGCCTC CTCCGTCGTG 
TCCAGTGGCG GTATAAACTC CGCCCCTGCT GTGATCACAA GCCCCGCCTC CCCAACCTTC 
ACCTTTCCCC TCAACCGCCT TTTCTCCCAC AACTGCAGTG ATCCTAAATC TAATCGTCGC 
TCCTCGTACC TGTTGGCCAT CACCACTGAA CGCTCCAAGT CATGTGATGA GGGGCTTAAC 
GCATATAGAG AAGAGGGCCG GGTCTTCTCG AGGCTTCCAA AAAGAGTCAA GAGCTTCTTT 
ACTGAAGGGT CTCTGGACAG TCTGGGTGCT GCTGAAGAGG CTCGCTCTAA ACGACACTCT 
ACCTCTGAGC TGGGCAACAT CAGCTACAGT GACGTGAAGA GAGAAGGCTG GCTCCACTTC 
AAACAGATCC ACACGGAGAA GGGCAAGAAG GTTGGCAGTG CAATCTGGCC ATGGAGACGG 
GTGTTCTCTG TGCTTAGGAC CCATTCTCTG TTTCTTTATA AAGACAAGCG AGAGGCAGTG 
CTTAAAGGGG CGCAATTAGG GGGCGGGGCT GAAGACGAGC AGCCAATCAG CATCCGGGGC 
TGCCTTGTGG ATATAGCATA CAGTGAAACG AAGCGCAAGC ACGCGCTGAG ATTGACCACG 
CAGGACTTCT GCGAGTACCT GCTGCAGGCG GAGGACAGAG AGGACATGCT GGAGTGGATC 
CGAGTCATCA GGGACAACAG CAAGACCGAC AACGAGGAAT TGGGTTTCTC ACGACAAGCT 
CTCATCAATA AAAAGCTTAA CGACTACAGG AAGCAAAGTC CAACGGGTAA CAAACCAGAC 
TCCTCACCCA GAGTGCATCG AATGACCTTC CTCCTCAGCA AGCCAGACAA CAGCGCACCT 
CCACGCTCCC CAAAACACGA GGCCAAAGAT GAGAGCAGCC CCCCAAAATC TCCCTGGGGT 
ATAAACATAA TGAAGAAGAG CAAGAAGGCC GGTCCTAAAG CTTTTGGCGT CCGTCTAGAG 
GACTGCCAGC CTGCAGTAAA CAACAAGTTC ATTCCTCAGA TTGTGGAGAT CTGCTGTGGG 
CTGGTGGAGG ACATGGGTCT GGAGTATACA GGGATCTACA GAGTGCCAGG AAACAATGCT 
GTAGTCTCCA GTCTGCAGGA GCAGCTCAAC AAAGGAGTAG ACATTAACAT CACTGAGGAG 
AAATGGCAGG ACCTGAATGT GGTGAGCAGC CTGCTGAAGT CCTTCTTTCG TAAACTCCCT 
GAACCCCTCT TCACTGATGA TAAATATAAT GACTTTATCG ACGCCAATCG GATGGAGAAC 
GCCAGCGATA GGCTGAGGAC AATGAAGAAA TTGATTCGGG ACCTACCAGA TCATTACTTT 
CACACTCTGA AATTTTTAGT TGGACACCTA AAGACGGTGG CTGACCACTC TGAGAAAAAC 
AAAATGGAGC CGCGTAATTT GGCTCTGGTG TTTGGCCCCA CTCTTGTTCG AACTTCAGAG 
GACAACATGA CAGACATGGT CACCCACATG CCAGACCGCT ACAAGATTGT GGAGACACTC 
ATTCAGCATT ACACCTGGTT TTTCTGCGAA GAACAGGATA AGGATGAAAA GACCCCAGTG 
GACACAGAGG ATGTCCAGCC TGCCCCAAAC ATTGATCATC TGCTCTCCAA CATTGGCCGC 
ACAGGCATGC TGGGTGAGGC TTCAGACTCA ACCAACAGTG ATTCTGCAAA ATCTAAGGGG 
TCATGGGGAT CAAAACGTGA CTTTACTGCC AAGGATATCC TTACATTATC AATTATGTCG 
GCTGTGACCC GCAAACGGCG GAAGCGGCAC AACGGCCGTC TCCTCGGCAG CAGCACAGAC 
GACGATTCTG AGCATGAACC CATTAAATCC TGCATCTCCG AGAAAGACGA TCTGCAAGAG 
ATGGGGCAGG TTGCATCCTT CTGTGCCCCA CGAGCAGAGG GAGAGGAGGA TGACGATGAA 
GAGGAGGAAG ATGATGAAGA GGAGGAGGAG GGGGCTGAGA GACAGAAGGC ACAAGAGCAG 
AAGGAAACAG TAGTCCCCAG CATTTCCTCA ACAGAGGAAG TGACATCAAC AGTGCTGCTG 
AGCGAAGAGG AAGAGCAGAA GTCTGAAGTG AAAGGAGGAC GCAGCTGGAG ATTAGATGAT 
GCTCGGTCTA TTGTGTCAGG CTACTCTACT CTGTCCACTT TGGGGCGGAG CTTAGCTTCA 
GAGGGGCGGG GCGAGGATGC AGATGATGAG CATAGTGAAC TAGTGAGTGA AACTGATAAT 
GAAAGCGGGT TTGCCTCACG CTCCCTCACC CAAGAAAGGC CAGAAAAACC AAACCGTTCC 
AACCAGAACT CTCATGCGTC TCCTGAACCA ACCGCACCAC AGAGTTTCCT CTATGCGCAC 
TGCAAATCAC CTACACCCTC TGGCTCCGCC ACTGCTCCAT CTCCCCCTTC ACAACACATC 
ACAACCAACC CAGAGGACAG CGCAGCACGC TCGTCTACTC CCTCAACCTC CTCTTACTCC 
TCATCCGCCT CACAACGGCT CCACAACCGC TCTTTCAACT CCCACCGCCT CATACAGTGC 
GACACCCTTG CACGCCGTCG GACAAAGGCG GACAAAGCCA AAGCTCCCTC AGTGGACCTT 
TCCGAACTCT CGAGCTCCTC CGCCGCAGAG GAGGACCGCC AGTCAGGTGC CAATCAAATC 
CCTGACTCCT CTCCGTCTCC TTCTTCAAAA GCCAAAGGGT TTAGCATAAG AAACGAGTGT 
ACGCCAATAT CCATGGTTGG TCCCGGAAGT AAGGGGTGTG TGTTGTCCCC CGGGCAAGGA 
TCCCTGGCGG ATCAGGTACG TGCTCGTCTA ATGGGCTCGG CGGATGACCT GTGCAGCGTT 
GGCCTGAGAA AGCAGCTCTC GCCGGAGACG CGCCGGAAGA GACGCGCTTG GAGGAGACAC 
ACAGTGGTCG TGTCCCCTAC GGATTGTTCA GACAAAAAGC CAACGGTCCA AAGCACCAAA 
CCTCTAGCTT CACCCTCCCC TGTTGAAAAG CAGGATGTCA GAGAGTCCCA CCCAGAGTGC 
TCAGAAACTG AAGCCCCTAC TGCACGTCGG GCCGTGCCTA CCTCGCGCTT TCGTGATTTC 
TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164232.1
CDS	301..4986
Translation	MGQNQLDNWSRWPGSSAGPSPPLDNRTVASSNVVWAAEGRNCEPGGVGHSSPAHRTEEIQYGMTDRGQQPTGQMRGRSYSSSSSSGGMMSPLHHGHLANHNMGTAMGHTGGRKGSPPWASPSQPTSTPSSSRNERTQQALSNWYYSQVPGRERERERERERSSSRSMHPRHRSFSQDRLGELGKARRSQQAGFPHSASQDTLLLLQQSAAAYADPYWNHGDWRGEHMYGQHPSVVAYGRKSSENLLASRYGHSGRSLENLDRAVGILSPRVERSAWVQQPPNRTEVYARHAGHFGQHIESPHQMHHTPQHHQNLTHATHQHSSQIPQPQQAAPMARRLPSCQSLDEEPIGYRSYSPSFNRKTGRILQQAQSFRDPSYTGPHLWTPTSKTSPPEGHPPSATASPAPPTTSSSTTISTPSEGQDRTYRPTNHDRGAGEATSEAQTQEVVLRQKPPTGRRGANALRHPHYAIALDSSDPLLFTTDPTEPTAKAESAISHRRTDVNLPPLSIEDDSLASIPFIDEPTSPSADLRARHVPASSVVSSGGINSAPAVITSPASPTFTFPLNRLFSHNCSDPKSNRRSSYLLAITTERSKSCDEGLNAYREEGRVFSRLPKRVKSFFTEGSLDSLGAAEEARSKRHSTSELGNISYSDVKREGWLHFKQIHTEKGKKVGSAIWPWRRVFSVLRTHSLFLYKDKREAVLKGAQLGGGAEDEQPISIRGCLVDIAYSETKRKHALRLTTQDFCEYLLQAEDREDMLEWIRVIRDNSKTDNEELGFSRQALINKKLNDYRKQSPTGNKPDSSPRVHRMTFLLSKPDNSAPPRSPKHEAKDESSPPKSPWGINIMKKSKKAGPKAFGVRLEDCQPAVNNKFIPQIVEICCGLVEDMGLEYTGIYRVPGNNAVVSSLQEQLNKGVDINITEEKWQDLNVVSSLLKSFFRKLPEPLFTDDKYNDFIDANRMENASDRLRTMKKLIRDLPDHYFHTLKFLVGHLKTVADHSEKNKMEPRNLALVFGPTLVRTSEDNMTDMVTHMPDRYKIVETLIQHYTWFFCEEQDKDEKTPVDTEDVQPAPNIDHLLSNIGRTGMLGEASDSTNSDSAKSKGSWGSKRDFTAKDILTLSIMSAVTRKRRKRHNGRLLGSSTDDDSEHEPIKSCISEKDDLQEMGQVASFCAPRAEGEEDDDEEEEDDEEEEEGAERQKAQEQKETVVPSISSTEEVTSTVLLSEEEEQKSEVKGGRSWRLDDARSIVSGYSTLSTLGRSLASEGRGEDADDEHSELVSETDNESGFASRSLTQERPEKPNRSNQNSHASPEPTAPQSFLYAHCKSPTPSGSATAPSPPSQHITTNPEDSAARSSTPSTSSYSSSASQRLHNRSFNSHRLIQCDTLARRRTKADKAKAPSVDLSELSSSSAAEEDRQSGANQIPDSSPSPSSKAKGFSIRNECTPISMVGPGSKGCVLSPGQGSLADQVRARLMGSADDLCSVGLRKQLSPETRRKRRAWRRHTVVVSPTDCSDKKPTVQSTKPLASPSPVEKQDVRESHPECSETEAPTARRAVPTSRFRDFL

Target ORF information:

RefSeq Version	XM_005164175.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Rho GTPase activating protein 23a (arhgap23a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164175.4

ORF Insert Sequence:

ATGGGGCAAA ACCAGCTGGA CAACTGGAGC CGCTGGCCCG GCTCTTCTGC TGGTCCCTCT 
CCACCCCTTG ACAACCGGAC TGTGGCCTCC AGCAATGTTG TCTGGGCAGC CGAGGGCAGG 
AATTGTGAGC CGGGTGGTGT TGGTCACAGT AGCCCTGCAC ACCGAACAGA GGAGATCCAG 
TATGGAATGA CTGACAGAGG ACAGCAGCCA ACTGGACAAA TGAGAGGACG CTCATACTCA 
TCTTCTTCCT CCTCAGGAGG AATGATGAGC CCTTTGCATC ATGGTCATCT TGCCAATCAC 
AACATGGGTA CTGCCATGGG GCACACTGGA GGTCGTAAGG GAAGCCCACC TTGGGCCAGC 
CCTTCTCAGC CAACATCAAC ACCTTCATCC AGCCGCAACG AACGAACCCA ACAAGCCCTT 
TCCAACTGGT ACTACAGCCA GGTCCCAGGC CGTGAGCGAG AACGAGAACG AGAGCGGGAG 
CGATCTAGTT CTCGCTCAAT GCACCCACGC CACAGGAGTT TCTCACAAGA CCGTCTGGGA 
GAGCTTGGGA AGGCCAGGCG TTCTCAACAA GCAGGTTTTC CCCATAGTGC ATCGCAAGAC 
ACGTTGCTGC TGCTTCAGCA GAGTGCTGCA GCATATGCTG ATCCTTACTG GAACCATGGA 
GACTGGCGGG GTGAGCATAT GTATGGTCAG CACCCATCTG TGGTGGCCTA TGGGCGAAAG 
TCCTCCGAAA ATCTTCTAGC AAGCCGGTAC GGACACTCGG GACGCTCACT GGAAAATCTC 
GACAGAGCTG TGGGGATACT CTCACCAAGG GTTGAAAGAT CTGCTTGGGT TCAACAACCT 
CCAAATAGGA CTGAAGTCTA TGCAAGACAT GCTGGGCATT TCGGCCAACA TATAGAGTCA 
CCCCACCAAA TGCACCACAC ACCCCAACAC CACCAGAATC TCACACATGC AACACACCAA 
CACTCATCTC AAATCCCACA ACCTCAGCAG GCAGCTCCAA TGGCAAGACG TTTGCCTTCT 
TGCCAGAGTC TAGATGAGGA GCCGATTGGC TATCGTAGCT ACAGTCCCTC ATTCAATAGG 
AAGACTGGCC GTATCTTGCA GCAGGCCCAG TCATTTAGAG ATCCCTCTTA CACAGGTCCA 
CACCTTTGGA CTCCAACATC CAAAACCAGT CCCCCAGAGG GGCATCCACC CTCAGCCACT 
GCTTCCCCAG CCCCACCAAC CACTTCCTCT TCAACTACGA TTTCCACACC ATCTGAAGGA 
CAAGACAGAA CTTACCGACC CACTAATCAT GACAGAGGGG CAGGGGAAGC GACAAGTGAA 
GCCCAGACAC AGGAAGTGGT TCTGAGGCAG AAACCACCTA CCGGCCGACG AGGTGCAAAC 
GCCCTTCGAC ACCCACATTA TGCAATAGCA CTTGACTCCA GTGACCCTCT ACTCTTTACC 
ACTGACCCTA CGGAGCCCAC TGCCAAGGCA GAAAGTGCTA TTTCTCATCG CCGCACAGAT 
GTTAACCTTC CTCCACTATC TATTGAGGAT GACTCCCTTG CATCAATTCC ATTCATCGAT 
GAGCCGACCA GTCCAAGTGC AGATCTGCGG GCACGTCATG TCCCCGCCTC CTCCGTCGTG 
TCCAGTGGCG GTATAAACTC CGCCCCTGCT GTGATCACAA GCCCCGCCTC CCCAACCTTC 
ACCTTTCCCC TCAACCGCCT TTTCTCCCAC AACTGCAGTG ATCCTAAATC TAATCGTCGC 
TCCTCGTACC TGTTGGCCAT CACCACTGAA CGCTCCAAGT CATGTGATGA GGGGCTTAAC 
GCATATAGAG AAGAGGGCCG GGTCTTCTCG AGGCTTCCAA AAAGAGTCAA GAGCTTCTTT 
ACTGAAGGGT CTCTGGACAG TCTGGGTGCT GCTGAAGAGG CTCGCTCTAA ACGACACTCT 
ACCTCTGAGC TGGGCAACAT CAGCTACAGT GACGTGAAGA GAGAAGGCTG GCTCCACTTC 
AAACAGATCC ACACGGAGAA GGGCAAGAAG GTTGGCAGTG CAATCTGGCC ATGGAGACGG 
GTGTTCTCTG TGCTTAGGAC CCATTCTCTG TTTCTTTATA AAGACAAGCG AGAGGCAGTG 
CTTAAAGGGG CGCAATTAGG GGGCGGGGCT GAAGACGAGC AGCCAATCAG CATCCGGGGC 
TGCCTTGTGG ATATAGCATA CAGTGAAACG AAGCGCAAGC ACGCGCTGAG ATTGACCACG 
CAGGACTTCT GCGAGTACCT GCTGCAGGCG GAGGACAGAG AGGACATGCT GGAGTGGATC 
CGAGTCATCA GGGACAACAG CAAGACCGAC AACGAGGAAT TGGGTTTCTC ACGACAAGCT 
CTCATCAATA AAAAGCTTAA CGACTACAGG AAGCAAAGTC CAACGGGTAA CAAACCAGAC 
TCCTCACCCA GAGTGCATCG AATGACCTTC CTCCTCAGCA AGCCAGACAA CAGCGCACCT 
CCACGCTCCC CAAAACACGA GGCCAAAGAT GAGAGCAGCC CCCCAAAATC TCCCTGGGGT 
ATAAACATAA TGAAGAAGAG CAAGAAGGCC GGTCCTAAAG CTTTTGGCGT CCGTCTAGAG 
GACTGCCAGC CTGCAGTAAA CAACAAGTTC ATTCCTCAGA TTGTGGAGAT CTGCTGTGGG 
CTGGTGGAGG ACATGGGTCT GGAGTATACA GGGATCTACA GAGTGCCAGG AAACAATGCT 
GTAGTCTCCA GTCTGCAGGA GCAGCTCAAC AAAGGAGTAG ACATTAACAT CACTGAGGAG 
AAATGGCAGG ACCTGAATGT GGTGAGCAGC CTGCTGAAGT CCTTCTTTCG TAAACTCCCT 
GAACCCCTCT TCACTGATGA TAAATATAAT GACTTTATCG ACGCCAATCG GATGGAGAAC 
GCCAGCGATA GGCTGAGGAC AATGAAGAAA TTGATTCGGG ACCTACCAGA TCATTACTTT 
CACACTCTGA AATTTTTAGT TGGACACCTA AAGACGGTGG CTGACCACTC TGAGAAAAAC 
AAAATGGAGC CGCGTAATTT GGCTCTGGTG TTTGGCCCCA CTCTTGTTCG AACTTCAGAG 
GACAACATGA CAGACATGGT CACCCACATG CCAGACCGCT ACAAGATTGT GGAGACACTC 
ATTCAGCATT ACACCTGGTT TTTCTGCGAA GAACAGGATA AGGATGAAAA GACCCCAGTG 
GACACAGAGG ATGTCCAGCC TGCCCCAAAC ATTGATCATC TGCTCTCCAA CATTGGCCGC 
ACAGGCATGC TGGGTGAGGC TTCAGACTCA ACCAACAGTG ATTCTGCAAA ATCTAAGGGG 
TCATGGGGAT CAAAACGTGA CTTTACTGCC AAGGATATCC TTACATTATC AATTATGTCG 
GCTGTGACCC GCAAACGGCG GAAGCGGCAC AACGGCCGTC TCCTCGGCAG CAGCACAGAC 
GACGATTCTG AGCATGAACC CATTAAATCC TGCATCTCCG AGAAAGACGA TCTGCAAGAG 
ATGGGGCAGG TTGCATCCTT CTGTGCCCCA CGAGCAGAGG GAGAGGAGGA TGACGATGAA 
GAGGAGGAAG ATGATGAAGA GGAGGAGGAG GGGGCTGAGA GACAGAAGGC ACAAGAGCAG 
AAGGAAACAG TAGTCCCCAG CATTTCCTCA ACAGAGGAAG TGACATCAAC AGTGCTGCTG 
AGCGAAGAGG AAGAGCAGAA GTCTGAAGTG AAAGGAGGAC GCAGCTGGAG ATTAGATGAT 
GCTCGGTCTA TTGTGTCAGG CTACTCTACT CTGTCCACTT TGGGGCGGAG CTTAGCTTCA 
GAGGGGCGGG GCGAGGATGC AGATGATGAG CATAGTGAAC TAGTGAGTGA AACTGATAAT 
GAAAGCGGGT TTGCCTCACG CTCCCTCACC CAAGAAAGGC CAGAAAAACC AAACCGTTCC 
AACCAGAACT CTCATGCGTC TCCTGAACCA ACCGCACCAC AGAGTTTCCT CTATGCGCAC 
TGCAAATCAC CTACACCCTC TGGCTCCGCC ACTGCTCCAT CTCCCCCTTC ACAACACATC 
ACAACCAACC CAGAGGACAG CGCAGCACGC TCGTCTACTC CCTCAACCTC CTCTTACTCC 
TCATCCGCCT CACAACGGCT CCACAACCGC TCTTTCAACT CCCACCGCCT CATACAGTGC 
GACACCCTTG CACGCCGTCG GACAAAGGCG GACAAAGCCA AAGCTCCCTC AGTGGACCTT 
TCCGAACTCT CGAGCTCCTC CGCCGCAGAG GAGGACCGCC AGTCAGGTGC CAATCAAATC 
CCTGACTCCT CTCCGTCTCC TTCTTCAAAA GCCAAAGGGT TTAGCATAAG AAACGAGTGT 
ACGCCAATAT CCATGGTTGG TCCCGGAAGT AAGGGGTGTG TGTTGTCCCC CGGGCAAGGA 
TCCCTGGCGG ATCAGGTACG TGCTCGTCTA ATGGGCTCGG CGGATGACCT GTGCAGCGTT 
GGCCTGAGAA AGCAGCTCTC GCCGGAGACG CGCCGGAAGA GACGCGCTTG GAGGAGACAC 
ACAGTGGTCG TGTCCCCTAC GGATTGTTCA GACAAAAAGC CAACGGTCCA AAGCACCAAA 
CCTCTAGCTT CACCCTCCCC TGTTGAAAAG CAGGATGTCA GAGAGTCCCA CCCAGAGTGC 
TCAGAAACTG AAGCCCCTAC TGCACGTCGG GCCGTGCCTA CCTCGCGCTT TCGTGATTTC 
TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap23a ( XM_005164176.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005164233.1 Danio rerio Rho GTPase activating protein 23a (arhgap23a), transcript variant X6, mRNA.

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CloneID	ODa08643
Clone ID Related Accession (Same CDS sequence)	XM_005164176.4 , XM_005164176.3
Accession Version	XM_005164176.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	rho GTPase-activating protein 23 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005164176.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCAG CAGGTCAAGT GCCACTGGCG ATGCCCGTTG TGTGCCCGCA TGTAGGGGGT 
AAGGAGTGGT GCTTTCAGGA TGCAGTCGGG GTGGATTGCA GCTCTCCAGA GCCTCGCTGT 
ATCTGGGTGG CGGTTTTACA CGGGGATACA CAGCAAATTA ACATTTCCAA CCTCCAAAAT 
GACTCTTCTC CAAACATAAA TGGCATCAGT GCTGGGAAAG CACTGTCAAG ACCCGCACAG 
ACCTCCAGGG CAAAAGGGCG GAGAGATGGG CTCTCTTCAT CCAGTGAGAA CCCTCGGCCG 
GTGGTCCGGA GCGGGACAGA TGGGCTGGGC GTGTCCTGGC AGGGTCCACG CACGCTGGTG 
CTCCAAAAGA ACTCTCAGGG CTTCGGCTTT ACTCTGCGTC ACTTCATCGT TTACCCGCCA 
GAGTCCGCTC TGCACACCAC TCTCAAGGAT GAAGAGAACG GGAATGGAAA GGGGCTTCAA 
CGAAGTCGTC TGGAGCCCAT GGACACAATT TTTGTGAAGA ATGTGAGGGA AAAAGGCCCC 
GCTCACCTGG CTGGCCTGTG TACAGGAGAC AGGCTGGTTA AAGTGAATGG GGAGAGCGTG 
CTGGGTAAGA CGTACTCGCA GGTCATTGCA CTGATTCAGA ACAGTGAGAG TGTACTGGAG 
CTCTCCATTA TGCCAAAGGA TGAGGACGTG CTTCAGCTGG CATATTCCCA TGATGCCTAC 
CTAAGGGGGA ACAATCCATA TACAGGAAAG GCCCAAAATC TTCCTCCACC ACCCCCTGTT 
CTCTACCCTC CTCGTACAAA ATCACAGTCT CCTGCAGGGT TCAGCCCCTG CCCTCCTCCA 
GCTTCTTCAC ACATGGGGCA AAACCAGCTG GACAACTGGA GCCGCTGGCC CGGCTCTTCT 
GCTGGTCCCT CTCCACCCCT TGACAACCGG ACTGTGGCCT CCAGCAATGT TGTCTGGGCA 
GCCGAGGGCA GGAATTGTGA GCCGGGTGGT GTTGGTCACA GTAGCCCTGC ACACCGAACA 
GAGGAGATCC AGTATGGAAT GACTGACAGA GGACAGCAGC CAACTGGACA AATGAGAGGA 
CGCTCATACT CATCTTCTTC CTCCTCAGGA GGAATGATGA GCCCTTTGCA TCATGGTCAT 
CTTGCCAATC ACAACATGGG TACTGCCATG GGGCACACTG GAGGTCGTAA GGGAAGCCCA 
CCTTGGGCCA GCCCTTCTCA GCCAACATCA ACACCTTCAT CCAGCCGCAA CGAACGAACC 
CAACAAGCCC TTTCCAACTG GTACTACAGC CAGGTCCCAG GCCGTGAGCG AGAACGAGAA 
CGAGAGCGGG AGCGATCTAG TTCTCGCTCA ATGCACCCAC GCCACAGGAG TTTCTCACAA 
GACCGTCTGG GAGAGCTTGG GAAGGCCAGG CGTTCTCAAC AAGCAGGTTT TCCCCATAGT 
GCATCGCAAG ACACGTTGCT GCTGCTTCAG CAGAGTGCTG CAGCATATGC TGATCCTTAC 
TGGAACCATG GAGACTGGCG GGGTGAGCAT ATGTATGGTC AGCACCCATC TGTGGTGGCC 
TATGGGCGAA AGTCCTCCGA AAATCTTCTA GCAAGCCGGT ACGGACACTC GGGACGCTCA 
CTGGAAAATC TCGACAGAGC TGTGGGGATA CTCTCACCAA GGGTTGAAAG ATCTGCTTGG 
GTTCAACAAC CTCCAAATAG GACTGAAGTC TATGCAAGAC ATGCTGGGCA TTTCGGCCAA 
CATATAGAGT CACCCCACCA AATGCACCAC ACACCCCAAC ACCACCAGAA TCTCACACAT 
GCAACACACC AACACTCATC TCAAATCCCA CAACCTCAGC AGGCAGCTCC AATGGCAAGA 
CGTTTGCCTT CTTGCCAGAG TCTAGATGAG GAGCCGATTG GCTATCGTAG CTACAGTCCC 
TCATTCAATA GGAAGACTGG CCGTATCTTG CAGCAGGCCC AGTCATTTAG AGATCCCTCT 
TACACAGGTC CACACCTTTG GACTCCAACA TCCAAAACCA GTCCCCCAGA GGGGCATCCA 
CCCTCAGCCA CTGCTTCCCC AGCCCCACCA ACCACTTCCT CTTCAACTAC GATTTCCACA 
CCATCTGAAG GACAAGACAG AACTTACCGA CCCACTAATC ATGACAGAGG GGCAGGGGAA 
GCGACAAGTG AAGCCCAGAC ACAGGAAGTG GTTCTGAGGC AGAAACCACC TACCGGCCGA 
CGAGGTGCAA ACGCCCTTCG ACACCCACAT TATGCAATAG CACTTGACTC CAGTGACCCT 
CTACTCTTTA CCACTGACCC TACGGAGCCC ACTGCCAAGG CAGAAAGTGC TATTTCTCAT 
CGCCGCACAG ATGTTAACCT TCCTCCACTA TCTATTGAGG ATGACTCCCT TGCATCAATT 
CCATTCATCG ATGAGCCGAC CAGTCCAAGT GCAGATCTGC GGGCACGTCA TGTCCCCGCC 
TCCTCCGTCG TGTCCAGTGG CGGTATAAAC TCCGCCCCTG CTGTGATCAC AAGCCCCGCC 
TCCCCAACCT TCACCTTTCC CCTCAACCGC CTTTTCTCCC ACAACTGCAG TGATCCTAAA 
TCTAATCGTC GCTCCTCGTA CCTGTTGGCC ATCACCACTG AACGCTCCAA GTCATGTGAT 
GAGGGGCTTA ACGCATATAG AGAAGAGGGC CGGGTCTTCT CGAGGCTTCC AAAAAGAGTC 
AAGAGCTTCT TTACTGAAGG GTCTCTGGAC AGTCTGGGTG CTGCTGAAGA GGCTCGCTCT 
AAACGACACT CTACCTCTGA GCTGGGCAAC ATCAGCTACA GTGACGTGAA GAGAGAAGGC 
TGGCTCCACT TCAAACAGAT CCACACGGAG AAGGGCAAGA AGGTTGGCAG TGCAATCTGG 
CCATGGAGAC GGGTGTTCTC TGTGCTTAGG ACCCATTCTC TGTTTCTTTA TAAAGACAAG 
CGAGAGGCAG TGCTTAAAGG GGCGCAATTA GGGGGCGGGG CTGAAGACGA GCAGCCAATC 
AGCATCCGGG GCTGCCTTGT GGATATAGCA TACAGTGAAA CGAAGCGCAA GCACGCGCTG 
AGATTGACCA CGCAGGACTT CTGCGAGTAC CTGCTGCAGG CGGAGGACAG AGAGGACATG 
CTGGAGTGGA TCCGAGTCAT CAGGGACAAC AGCAAGACCG ACAACGAGGA ATTGGGTTTC 
TCACGACAAG CTCTCATCAA TAAAAAGCTT AACGACTACA GGAAGCAAAG TCCAACGGGT 
AACAAACCAG ACTCCTCACC CAGAGTGCAT CGAATGACCT TCCTCCTCAG CAAGCCAGAC 
AACAGCGCAC CTCCACGCTC CCCAAAACAC GAGGCCAAAG ATGAGAGCAG CCCCCCAAAA 
TCTCCCTGGG GTATAAACAT AATGAAGAAG AGCAAGAAGG CCGGTCCTAA AGCTTTTGGC 
GTCCGTCTAG AGGACTGCCA GCCTGCAGTA AACAACAAGT TCATTCCTCA GATTGTGGAG 
ATCTGCTGTG GGCTGGTGGA GGACATGGGT CTGGAGTATA CAGGGATCTA CAGAGTGCCA 
GGAAACAATG CTGTAGTCTC CAGTCTGCAG GAGCAGCTCA ACAAAGGAGT AGACATTAAC 
ATCACTGAGG AGAAATGGCA GGACCTGAAT GTGGTGAGCA GCCTGCTGAA GTCCTTCTTT 
CGTAAACTCC CTGAACCCCT CTTCACTGAT GATAAATATA ATGACTTTAT CGACGCCAAT 
CGGATGGAGA ACGCCAGCGA TAGGCTGAGG ACAATGAAGA AATTGATTCG GGACCTACCA 
GATCATTACT TTCACACTCT GAAATTTTTA GTTGGACACC TAAAGACGGT GGCTGACCAC 
TCTGAGAAAA ACAAAATGGA GCCGCGTAAT TTGGCTCTGG TGTTTGGCCC CACTCTTGTT 
CGAACTTCAG AGGACAACAT GACAGACATG GTCACCCACA TGCCAGACCG CTACAAGATT 
GTGGAGACAC TCATTCAGCA TTACACCTGG TTTTTCTGCG AAGAACAGGA TAAGGATGAA 
AAGACCCCAG TGGACACAGA GGATGTCCAG CCTGCCCCAA ACATTGATCA TCTGCTCTCC 
AACATTGGCC GCACAGGCAT GCTGGGTGAG GCTTCAGCTG AGCCTGTGGA ACAGCCACTG 
CGACTCAACC AACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164233.1
CDS	993..5150
Translation	MWSAGQVPLAMPVVCPHVGGKEWCFQDAVGVDCSSPEPRCIWVAVLHGDTQQINISNLQNDSSPNINGISAGKALSRPAQTSRAKGRRDGLSSSSENPRPVVRSGTDGLGVSWQGPRTLVLQKNSQGFGFTLRHFIVYPPESALHTTLKDEENGNGKGLQRSRLEPMDTIFVKNVREKGPAHLAGLCTGDRLVKVNGESVLGKTYSQVIALIQNSESVLELSIMPKDEDVLQLAYSHDAYLRGNNPYTGKAQNLPPPPPVLYPPRTKSQSPAGFSPCPPPASSHMGQNQLDNWSRWPGSSAGPSPPLDNRTVASSNVVWAAEGRNCEPGGVGHSSPAHRTEEIQYGMTDRGQQPTGQMRGRSYSSSSSSGGMMSPLHHGHLANHNMGTAMGHTGGRKGSPPWASPSQPTSTPSSSRNERTQQALSNWYYSQVPGRERERERERERSSSRSMHPRHRSFSQDRLGELGKARRSQQAGFPHSASQDTLLLLQQSAAAYADPYWNHGDWRGEHMYGQHPSVVAYGRKSSENLLASRYGHSGRSLENLDRAVGILSPRVERSAWVQQPPNRTEVYARHAGHFGQHIESPHQMHHTPQHHQNLTHATHQHSSQIPQPQQAAPMARRLPSCQSLDEEPIGYRSYSPSFNRKTGRILQQAQSFRDPSYTGPHLWTPTSKTSPPEGHPPSATASPAPPTTSSSTTISTPSEGQDRTYRPTNHDRGAGEATSEAQTQEVVLRQKPPTGRRGANALRHPHYAIALDSSDPLLFTTDPTEPTAKAESAISHRRTDVNLPPLSIEDDSLASIPFIDEPTSPSADLRARHVPASSVVSSGGINSAPAVITSPASPTFTFPLNRLFSHNCSDPKSNRRSSYLLAITTERSKSCDEGLNAYREEGRVFSRLPKRVKSFFTEGSLDSLGAAEEARSKRHSTSELGNISYSDVKREGWLHFKQIHTEKGKKVGSAIWPWRRVFSVLRTHSLFLYKDKREAVLKGAQLGGGAEDEQPISIRGCLVDIAYSETKRKHALRLTTQDFCEYLLQAEDREDMLEWIRVIRDNSKTDNEELGFSRQALINKKLNDYRKQSPTGNKPDSSPRVHRMTFLLSKPDNSAPPRSPKHEAKDESSPPKSPWGINIMKKSKKAGPKAFGVRLEDCQPAVNNKFIPQIVEICCGLVEDMGLEYTGIYRVPGNNAVVSSLQEQLNKGVDINITEEKWQDLNVVSSLLKSFFRKLPEPLFTDDKYNDFIDANRMENASDRLRTMKKLIRDLPDHYFHTLKFLVGHLKTVADHSEKNKMEPRNLALVFGPTLVRTSEDNMTDMVTHMPDRYKIVETLIQHYTWFFCEEQDKDEKTPVDTEDVQPAPNIDHLLSNIGRTGMLGEASAEPVEQPLRLNQQ

Target ORF information:

RefSeq Version	XM_005164176.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Rho GTPase activating protein 23a (arhgap23a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164176.4

ORF Insert Sequence:

ATGTGGTCAG CAGGTCAAGT GCCACTGGCG ATGCCCGTTG TGTGCCCGCA TGTAGGGGGT 
AAGGAGTGGT GCTTTCAGGA TGCAGTCGGG GTGGATTGCA GCTCTCCAGA GCCTCGCTGT 
ATCTGGGTGG CGGTTTTACA CGGGGATACA CAGCAAATTA ACATTTCCAA CCTCCAAAAT 
GACTCTTCTC CAAACATAAA TGGCATCAGT GCTGGGAAAG CACTGTCAAG ACCCGCACAG 
ACCTCCAGGG CAAAAGGGCG GAGAGATGGG CTCTCTTCAT CCAGTGAGAA CCCTCGGCCG 
GTGGTCCGGA GCGGGACAGA TGGGCTGGGC GTGTCCTGGC AGGGTCCACG CACGCTGGTG 
CTCCAAAAGA ACTCTCAGGG CTTCGGCTTT ACTCTGCGTC ACTTCATCGT TTACCCGCCA 
GAGTCCGCTC TGCACACCAC TCTCAAGGAT GAAGAGAACG GGAATGGAAA GGGGCTTCAA 
CGAAGTCGTC TGGAGCCCAT GGACACAATT TTTGTGAAGA ATGTGAGGGA AAAAGGCCCC 
GCTCACCTGG CTGGCCTGTG TACAGGAGAC AGGCTGGTTA AAGTGAATGG GGAGAGCGTG 
CTGGGTAAGA CGTACTCGCA GGTCATTGCA CTGATTCAGA ACAGTGAGAG TGTACTGGAG 
CTCTCCATTA TGCCAAAGGA TGAGGACGTG CTTCAGCTGG CATATTCCCA TGATGCCTAC 
CTAAGGGGGA ACAATCCATA TACAGGAAAG GCCCAAAATC TTCCTCCACC ACCCCCTGTT 
CTCTACCCTC CTCGTACAAA ATCACAGTCT CCTGCAGGGT TCAGCCCCTG CCCTCCTCCA 
GCTTCTTCAC ACATGGGGCA AAACCAGCTG GACAACTGGA GCCGCTGGCC CGGCTCTTCT 
GCTGGTCCCT CTCCACCCCT TGACAACCGG ACTGTGGCCT CCAGCAATGT TGTCTGGGCA 
GCCGAGGGCA GGAATTGTGA GCCGGGTGGT GTTGGTCACA GTAGCCCTGC ACACCGAACA 
GAGGAGATCC AGTATGGAAT GACTGACAGA GGACAGCAGC CAACTGGACA AATGAGAGGA 
CGCTCATACT CATCTTCTTC CTCCTCAGGA GGAATGATGA GCCCTTTGCA TCATGGTCAT 
CTTGCCAATC ACAACATGGG TACTGCCATG GGGCACACTG GAGGTCGTAA GGGAAGCCCA 
CCTTGGGCCA GCCCTTCTCA GCCAACATCA ACACCTTCAT CCAGCCGCAA CGAACGAACC 
CAACAAGCCC TTTCCAACTG GTACTACAGC CAGGTCCCAG GCCGTGAGCG AGAACGAGAA 
CGAGAGCGGG AGCGATCTAG TTCTCGCTCA ATGCACCCAC GCCACAGGAG TTTCTCACAA 
GACCGTCTGG GAGAGCTTGG GAAGGCCAGG CGTTCTCAAC AAGCAGGTTT TCCCCATAGT 
GCATCGCAAG ACACGTTGCT GCTGCTTCAG CAGAGTGCTG CAGCATATGC TGATCCTTAC 
TGGAACCATG GAGACTGGCG GGGTGAGCAT ATGTATGGTC AGCACCCATC TGTGGTGGCC 
TATGGGCGAA AGTCCTCCGA AAATCTTCTA GCAAGCCGGT ACGGACACTC GGGACGCTCA 
CTGGAAAATC TCGACAGAGC TGTGGGGATA CTCTCACCAA GGGTTGAAAG ATCTGCTTGG 
GTTCAACAAC CTCCAAATAG GACTGAAGTC TATGCAAGAC ATGCTGGGCA TTTCGGCCAA 
CATATAGAGT CACCCCACCA AATGCACCAC ACACCCCAAC ACCACCAGAA TCTCACACAT 
GCAACACACC AACACTCATC TCAAATCCCA CAACCTCAGC AGGCAGCTCC AATGGCAAGA 
CGTTTGCCTT CTTGCCAGAG TCTAGATGAG GAGCCGATTG GCTATCGTAG CTACAGTCCC 
TCATTCAATA GGAAGACTGG CCGTATCTTG CAGCAGGCCC AGTCATTTAG AGATCCCTCT 
TACACAGGTC CACACCTTTG GACTCCAACA TCCAAAACCA GTCCCCCAGA GGGGCATCCA 
CCCTCAGCCA CTGCTTCCCC AGCCCCACCA ACCACTTCCT CTTCAACTAC GATTTCCACA 
CCATCTGAAG GACAAGACAG AACTTACCGA CCCACTAATC ATGACAGAGG GGCAGGGGAA 
GCGACAAGTG AAGCCCAGAC ACAGGAAGTG GTTCTGAGGC AGAAACCACC TACCGGCCGA 
CGAGGTGCAA ACGCCCTTCG ACACCCACAT TATGCAATAG CACTTGACTC CAGTGACCCT 
CTACTCTTTA CCACTGACCC TACGGAGCCC ACTGCCAAGG CAGAAAGTGC TATTTCTCAT 
CGCCGCACAG ATGTTAACCT TCCTCCACTA TCTATTGAGG ATGACTCCCT TGCATCAATT 
CCATTCATCG ATGAGCCGAC CAGTCCAAGT GCAGATCTGC GGGCACGTCA TGTCCCCGCC 
TCCTCCGTCG TGTCCAGTGG CGGTATAAAC TCCGCCCCTG CTGTGATCAC AAGCCCCGCC 
TCCCCAACCT TCACCTTTCC CCTCAACCGC CTTTTCTCCC ACAACTGCAG TGATCCTAAA 
TCTAATCGTC GCTCCTCGTA CCTGTTGGCC ATCACCACTG AACGCTCCAA GTCATGTGAT 
GAGGGGCTTA ACGCATATAG AGAAGAGGGC CGGGTCTTCT CGAGGCTTCC AAAAAGAGTC 
AAGAGCTTCT TTACTGAAGG GTCTCTGGAC AGTCTGGGTG CTGCTGAAGA GGCTCGCTCT 
AAACGACACT CTACCTCTGA GCTGGGCAAC ATCAGCTACA GTGACGTGAA GAGAGAAGGC 
TGGCTCCACT TCAAACAGAT CCACACGGAG AAGGGCAAGA AGGTTGGCAG TGCAATCTGG 
CCATGGAGAC GGGTGTTCTC TGTGCTTAGG ACCCATTCTC TGTTTCTTTA TAAAGACAAG 
CGAGAGGCAG TGCTTAAAGG GGCGCAATTA GGGGGCGGGG CTGAAGACGA GCAGCCAATC 
AGCATCCGGG GCTGCCTTGT GGATATAGCA TACAGTGAAA CGAAGCGCAA GCACGCGCTG 
AGATTGACCA CGCAGGACTT CTGCGAGTAC CTGCTGCAGG CGGAGGACAG AGAGGACATG 
CTGGAGTGGA TCCGAGTCAT CAGGGACAAC AGCAAGACCG ACAACGAGGA ATTGGGTTTC 
TCACGACAAG CTCTCATCAA TAAAAAGCTT AACGACTACA GGAAGCAAAG TCCAACGGGT 
AACAAACCAG ACTCCTCACC CAGAGTGCAT CGAATGACCT TCCTCCTCAG CAAGCCAGAC 
AACAGCGCAC CTCCACGCTC CCCAAAACAC GAGGCCAAAG ATGAGAGCAG CCCCCCAAAA 
TCTCCCTGGG GTATAAACAT AATGAAGAAG AGCAAGAAGG CCGGTCCTAA AGCTTTTGGC 
GTCCGTCTAG AGGACTGCCA GCCTGCAGTA AACAACAAGT TCATTCCTCA GATTGTGGAG 
ATCTGCTGTG GGCTGGTGGA GGACATGGGT CTGGAGTATA CAGGGATCTA CAGAGTGCCA 
GGAAACAATG CTGTAGTCTC CAGTCTGCAG GAGCAGCTCA ACAAAGGAGT AGACATTAAC 
ATCACTGAGG AGAAATGGCA GGACCTGAAT GTGGTGAGCA GCCTGCTGAA GTCCTTCTTT 
CGTAAACTCC CTGAACCCCT CTTCACTGAT GATAAATATA ATGACTTTAT CGACGCCAAT 
CGGATGGAGA ACGCCAGCGA TAGGCTGAGG ACAATGAAGA AATTGATTCG GGACCTACCA 
GATCATTACT TTCACACTCT GAAATTTTTA GTTGGACACC TAAAGACGGT GGCTGACCAC 
TCTGAGAAAA ACAAAATGGA GCCGCGTAAT TTGGCTCTGG TGTTTGGCCC CACTCTTGTT 
CGAACTTCAG AGGACAACAT GACAGACATG GTCACCCACA TGCCAGACCG CTACAAGATT 
GTGGAGACAC TCATTCAGCA TTACACCTGG TTTTTCTGCG AAGAACAGGA TAAGGATGAA 
AAGACCCCAG TGGACACAGA GGATGTCCAG CCTGCCCCAA ACATTGATCA TCTGCTCTCC 
AACATTGGCC GCACAGGCAT GCTGGGTGAG GCTTCAGCTG AGCCTGTGGA ACAGCCACTG 
CGACTCAACC AACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap23a ( XM_005164176.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005164233.1 Danio rerio Rho GTPase activating protein 23a (arhgap23a), transcript variant X6, mRNA.

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CloneID	ODa08643
Clone ID Related Accession (Same CDS sequence)	XM_005164176.4 , XM_005164176.3
Accession Version	XM_005164176.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	rho GTPase-activating protein 23 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005164176.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCAG CAGGTCAAGT GCCACTGGCG ATGCCCGTTG TGTGCCCGCA TGTAGGGGGT 
AAGGAGTGGT GCTTTCAGGA TGCAGTCGGG GTGGATTGCA GCTCTCCAGA GCCTCGCTGT 
ATCTGGGTGG CGGTTTTACA CGGGGATACA CAGCAAATTA ACATTTCCAA CCTCCAAAAT 
GACTCTTCTC CAAACATAAA TGGCATCAGT GCTGGGAAAG CACTGTCAAG ACCCGCACAG 
ACCTCCAGGG CAAAAGGGCG GAGAGATGGG CTCTCTTCAT CCAGTGAGAA CCCTCGGCCG 
GTGGTCCGGA GCGGGACAGA TGGGCTGGGC GTGTCCTGGC AGGGTCCACG CACGCTGGTG 
CTCCAAAAGA ACTCTCAGGG CTTCGGCTTT ACTCTGCGTC ACTTCATCGT TTACCCGCCA 
GAGTCCGCTC TGCACACCAC TCTCAAGGAT GAAGAGAACG GGAATGGAAA GGGGCTTCAA 
CGAAGTCGTC TGGAGCCCAT GGACACAATT TTTGTGAAGA ATGTGAGGGA AAAAGGCCCC 
GCTCACCTGG CTGGCCTGTG TACAGGAGAC AGGCTGGTTA AAGTGAATGG GGAGAGCGTG 
CTGGGTAAGA CGTACTCGCA GGTCATTGCA CTGATTCAGA ACAGTGAGAG TGTACTGGAG 
CTCTCCATTA TGCCAAAGGA TGAGGACGTG CTTCAGCTGG CATATTCCCA TGATGCCTAC 
CTAAGGGGGA ACAATCCATA TACAGGAAAG GCCCAAAATC TTCCTCCACC ACCCCCTGTT 
CTCTACCCTC CTCGTACAAA ATCACAGTCT CCTGCAGGGT TCAGCCCCTG CCCTCCTCCA 
GCTTCTTCAC ACATGGGGCA AAACCAGCTG GACAACTGGA GCCGCTGGCC CGGCTCTTCT 
GCTGGTCCCT CTCCACCCCT TGACAACCGG ACTGTGGCCT CCAGCAATGT TGTCTGGGCA 
GCCGAGGGCA GGAATTGTGA GCCGGGTGGT GTTGGTCACA GTAGCCCTGC ACACCGAACA 
GAGGAGATCC AGTATGGAAT GACTGACAGA GGACAGCAGC CAACTGGACA AATGAGAGGA 
CGCTCATACT CATCTTCTTC CTCCTCAGGA GGAATGATGA GCCCTTTGCA TCATGGTCAT 
CTTGCCAATC ACAACATGGG TACTGCCATG GGGCACACTG GAGGTCGTAA GGGAAGCCCA 
CCTTGGGCCA GCCCTTCTCA GCCAACATCA ACACCTTCAT CCAGCCGCAA CGAACGAACC 
CAACAAGCCC TTTCCAACTG GTACTACAGC CAGGTCCCAG GCCGTGAGCG AGAACGAGAA 
CGAGAGCGGG AGCGATCTAG TTCTCGCTCA ATGCACCCAC GCCACAGGAG TTTCTCACAA 
GACCGTCTGG GAGAGCTTGG GAAGGCCAGG CGTTCTCAAC AAGCAGGTTT TCCCCATAGT 
GCATCGCAAG ACACGTTGCT GCTGCTTCAG CAGAGTGCTG CAGCATATGC TGATCCTTAC 
TGGAACCATG GAGACTGGCG GGGTGAGCAT ATGTATGGTC AGCACCCATC TGTGGTGGCC 
TATGGGCGAA AGTCCTCCGA AAATCTTCTA GCAAGCCGGT ACGGACACTC GGGACGCTCA 
CTGGAAAATC TCGACAGAGC TGTGGGGATA CTCTCACCAA GGGTTGAAAG ATCTGCTTGG 
GTTCAACAAC CTCCAAATAG GACTGAAGTC TATGCAAGAC ATGCTGGGCA TTTCGGCCAA 
CATATAGAGT CACCCCACCA AATGCACCAC ACACCCCAAC ACCACCAGAA TCTCACACAT 
GCAACACACC AACACTCATC TCAAATCCCA CAACCTCAGC AGGCAGCTCC AATGGCAAGA 
CGTTTGCCTT CTTGCCAGAG TCTAGATGAG GAGCCGATTG GCTATCGTAG CTACAGTCCC 
TCATTCAATA GGAAGACTGG CCGTATCTTG CAGCAGGCCC AGTCATTTAG AGATCCCTCT 
TACACAGGTC CACACCTTTG GACTCCAACA TCCAAAACCA GTCCCCCAGA GGGGCATCCA 
CCCTCAGCCA CTGCTTCCCC AGCCCCACCA ACCACTTCCT CTTCAACTAC GATTTCCACA 
CCATCTGAAG GACAAGACAG AACTTACCGA CCCACTAATC ATGACAGAGG GGCAGGGGAA 
GCGACAAGTG AAGCCCAGAC ACAGGAAGTG GTTCTGAGGC AGAAACCACC TACCGGCCGA 
CGAGGTGCAA ACGCCCTTCG ACACCCACAT TATGCAATAG CACTTGACTC CAGTGACCCT 
CTACTCTTTA CCACTGACCC TACGGAGCCC ACTGCCAAGG CAGAAAGTGC TATTTCTCAT 
CGCCGCACAG ATGTTAACCT TCCTCCACTA TCTATTGAGG ATGACTCCCT TGCATCAATT 
CCATTCATCG ATGAGCCGAC CAGTCCAAGT GCAGATCTGC GGGCACGTCA TGTCCCCGCC 
TCCTCCGTCG TGTCCAGTGG CGGTATAAAC TCCGCCCCTG CTGTGATCAC AAGCCCCGCC 
TCCCCAACCT TCACCTTTCC CCTCAACCGC CTTTTCTCCC ACAACTGCAG TGATCCTAAA 
TCTAATCGTC GCTCCTCGTA CCTGTTGGCC ATCACCACTG AACGCTCCAA GTCATGTGAT 
GAGGGGCTTA ACGCATATAG AGAAGAGGGC CGGGTCTTCT CGAGGCTTCC AAAAAGAGTC 
AAGAGCTTCT TTACTGAAGG GTCTCTGGAC AGTCTGGGTG CTGCTGAAGA GGCTCGCTCT 
AAACGACACT CTACCTCTGA GCTGGGCAAC ATCAGCTACA GTGACGTGAA GAGAGAAGGC 
TGGCTCCACT TCAAACAGAT CCACACGGAG AAGGGCAAGA AGGTTGGCAG TGCAATCTGG 
CCATGGAGAC GGGTGTTCTC TGTGCTTAGG ACCCATTCTC TGTTTCTTTA TAAAGACAAG 
CGAGAGGCAG TGCTTAAAGG GGCGCAATTA GGGGGCGGGG CTGAAGACGA GCAGCCAATC 
AGCATCCGGG GCTGCCTTGT GGATATAGCA TACAGTGAAA CGAAGCGCAA GCACGCGCTG 
AGATTGACCA CGCAGGACTT CTGCGAGTAC CTGCTGCAGG CGGAGGACAG AGAGGACATG 
CTGGAGTGGA TCCGAGTCAT CAGGGACAAC AGCAAGACCG ACAACGAGGA ATTGGGTTTC 
TCACGACAAG CTCTCATCAA TAAAAAGCTT AACGACTACA GGAAGCAAAG TCCAACGGGT 
AACAAACCAG ACTCCTCACC CAGAGTGCAT CGAATGACCT TCCTCCTCAG CAAGCCAGAC 
AACAGCGCAC CTCCACGCTC CCCAAAACAC GAGGCCAAAG ATGAGAGCAG CCCCCCAAAA 
TCTCCCTGGG GTATAAACAT AATGAAGAAG AGCAAGAAGG CCGGTCCTAA AGCTTTTGGC 
GTCCGTCTAG AGGACTGCCA GCCTGCAGTA AACAACAAGT TCATTCCTCA GATTGTGGAG 
ATCTGCTGTG GGCTGGTGGA GGACATGGGT CTGGAGTATA CAGGGATCTA CAGAGTGCCA 
GGAAACAATG CTGTAGTCTC CAGTCTGCAG GAGCAGCTCA ACAAAGGAGT AGACATTAAC 
ATCACTGAGG AGAAATGGCA GGACCTGAAT GTGGTGAGCA GCCTGCTGAA GTCCTTCTTT 
CGTAAACTCC CTGAACCCCT CTTCACTGAT GATAAATATA ATGACTTTAT CGACGCCAAT 
CGGATGGAGA ACGCCAGCGA TAGGCTGAGG ACAATGAAGA AATTGATTCG GGACCTACCA 
GATCATTACT TTCACACTCT GAAATTTTTA GTTGGACACC TAAAGACGGT GGCTGACCAC 
TCTGAGAAAA ACAAAATGGA GCCGCGTAAT TTGGCTCTGG TGTTTGGCCC CACTCTTGTT 
CGAACTTCAG AGGACAACAT GACAGACATG GTCACCCACA TGCCAGACCG CTACAAGATT 
GTGGAGACAC TCATTCAGCA TTACACCTGG TTTTTCTGCG AAGAACAGGA TAAGGATGAA 
AAGACCCCAG TGGACACAGA GGATGTCCAG CCTGCCCCAA ACATTGATCA TCTGCTCTCC 
AACATTGGCC GCACAGGCAT GCTGGGTGAG GCTTCAGCTG AGCCTGTGGA ACAGCCACTG 
CGACTCAACC AACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164233.1
CDS	1017..5174
Translation	MWSAGQVPLAMPVVCPHVGGKEWCFQDAVGVDCSSPEPRCIWVAVLHGDTQQINISNLQNDSSPNINGISAGKALSRPAQTSRAKGRRDGLSSSSENPRPVVRSGTDGLGVSWQGPRTLVLQKNSQGFGFTLRHFIVYPPESALHTTLKDEENGNGKGLQRSRLEPMDTIFVKNVREKGPAHLAGLCTGDRLVKVNGESVLGKTYSQVIALIQNSESVLELSIMPKDEDVLQLAYSHDAYLRGNNPYTGKAQNLPPPPPVLYPPRTKSQSPAGFSPCPPPASSHMGQNQLDNWSRWPGSSAGPSPPLDNRTVASSNVVWAAEGRNCEPGGVGHSSPAHRTEEIQYGMTDRGQQPTGQMRGRSYSSSSSSGGMMSPLHHGHLANHNMGTAMGHTGGRKGSPPWASPSQPTSTPSSSRNERTQQALSNWYYSQVPGRERERERERERSSSRSMHPRHRSFSQDRLGELGKARRSQQAGFPHSASQDTLLLLQQSAAAYADPYWNHGDWRGEHMYGQHPSVVAYGRKSSENLLASRYGHSGRSLENLDRAVGILSPRVERSAWVQQPPNRTEVYARHAGHFGQHIESPHQMHHTPQHHQNLTHATHQHSSQIPQPQQAAPMARRLPSCQSLDEEPIGYRSYSPSFNRKTGRILQQAQSFRDPSYTGPHLWTPTSKTSPPEGHPPSATASPAPPTTSSSTTISTPSEGQDRTYRPTNHDRGAGEATSEAQTQEVVLRQKPPTGRRGANALRHPHYAIALDSSDPLLFTTDPTEPTAKAESAISHRRTDVNLPPLSIEDDSLASIPFIDEPTSPSADLRARHVPASSVVSSGGINSAPAVITSPASPTFTFPLNRLFSHNCSDPKSNRRSSYLLAITTERSKSCDEGLNAYREEGRVFSRLPKRVKSFFTEGSLDSLGAAEEARSKRHSTSELGNISYSDVKREGWLHFKQIHTEKGKKVGSAIWPWRRVFSVLRTHSLFLYKDKREAVLKGAQLGGGAEDEQPISIRGCLVDIAYSETKRKHALRLTTQDFCEYLLQAEDREDMLEWIRVIRDNSKTDNEELGFSRQALINKKLNDYRKQSPTGNKPDSSPRVHRMTFLLSKPDNSAPPRSPKHEAKDESSPPKSPWGINIMKKSKKAGPKAFGVRLEDCQPAVNNKFIPQIVEICCGLVEDMGLEYTGIYRVPGNNAVVSSLQEQLNKGVDINITEEKWQDLNVVSSLLKSFFRKLPEPLFTDDKYNDFIDANRMENASDRLRTMKKLIRDLPDHYFHTLKFLVGHLKTVADHSEKNKMEPRNLALVFGPTLVRTSEDNMTDMVTHMPDRYKIVETLIQHYTWFFCEEQDKDEKTPVDTEDVQPAPNIDHLLSNIGRTGMLGEASAEPVEQPLRLNQQ

Target ORF information:

RefSeq Version	XM_005164176.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Rho GTPase activating protein 23a (arhgap23a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164176.3

ORF Insert Sequence:

ATGTGGTCAG CAGGTCAAGT GCCACTGGCG ATGCCCGTTG TGTGCCCGCA TGTAGGGGGT 
AAGGAGTGGT GCTTTCAGGA TGCAGTCGGG GTGGATTGCA GCTCTCCAGA GCCTCGCTGT 
ATCTGGGTGG CGGTTTTACA CGGGGATACA CAGCAAATTA ACATTTCCAA CCTCCAAAAT 
GACTCTTCTC CAAACATAAA TGGCATCAGT GCTGGGAAAG CACTGTCAAG ACCCGCACAG 
ACCTCCAGGG CAAAAGGGCG GAGAGATGGG CTCTCTTCAT CCAGTGAGAA CCCTCGGCCG 
GTGGTCCGGA GCGGGACAGA TGGGCTGGGC GTGTCCTGGC AGGGTCCACG CACGCTGGTG 
CTCCAAAAGA ACTCTCAGGG CTTCGGCTTT ACTCTGCGTC ACTTCATCGT TTACCCGCCA 
GAGTCCGCTC TGCACACCAC TCTCAAGGAT GAAGAGAACG GGAATGGAAA GGGGCTTCAA 
CGAAGTCGTC TGGAGCCCAT GGACACAATT TTTGTGAAGA ATGTGAGGGA AAAAGGCCCC 
GCTCACCTGG CTGGCCTGTG TACAGGAGAC AGGCTGGTTA AAGTGAATGG GGAGAGCGTG 
CTGGGTAAGA CGTACTCGCA GGTCATTGCA CTGATTCAGA ACAGTGAGAG TGTACTGGAG 
CTCTCCATTA TGCCAAAGGA TGAGGACGTG CTTCAGCTGG CATATTCCCA TGATGCCTAC 
CTAAGGGGGA ACAATCCATA TACAGGAAAG GCCCAAAATC TTCCTCCACC ACCCCCTGTT 
CTCTACCCTC CTCGTACAAA ATCACAGTCT CCTGCAGGGT TCAGCCCCTG CCCTCCTCCA 
GCTTCTTCAC ACATGGGGCA AAACCAGCTG GACAACTGGA GCCGCTGGCC CGGCTCTTCT 
GCTGGTCCCT CTCCACCCCT TGACAACCGG ACTGTGGCCT CCAGCAATGT TGTCTGGGCA 
GCCGAGGGCA GGAATTGTGA GCCGGGTGGT GTTGGTCACA GTAGCCCTGC ACACCGAACA 
GAGGAGATCC AGTATGGAAT GACTGACAGA GGACAGCAGC CAACTGGACA AATGAGAGGA 
CGCTCATACT CATCTTCTTC CTCCTCAGGA GGAATGATGA GCCCTTTGCA TCATGGTCAT 
CTTGCCAATC ACAACATGGG TACTGCCATG GGGCACACTG GAGGTCGTAA GGGAAGCCCA 
CCTTGGGCCA GCCCTTCTCA GCCAACATCA ACACCTTCAT CCAGCCGCAA CGAACGAACC 
CAACAAGCCC TTTCCAACTG GTACTACAGC CAGGTCCCAG GCCGTGAGCG AGAACGAGAA 
CGAGAGCGGG AGCGATCTAG TTCTCGCTCA ATGCACCCAC GCCACAGGAG TTTCTCACAA 
GACCGTCTGG GAGAGCTTGG GAAGGCCAGG CGTTCTCAAC AAGCAGGTTT TCCCCATAGT 
GCATCGCAAG ACACGTTGCT GCTGCTTCAG CAGAGTGCTG CAGCATATGC TGATCCTTAC 
TGGAACCATG GAGACTGGCG GGGTGAGCAT ATGTATGGTC AGCACCCATC TGTGGTGGCC 
TATGGGCGAA AGTCCTCCGA AAATCTTCTA GCAAGCCGGT ACGGACACTC GGGACGCTCA 
CTGGAAAATC TCGACAGAGC TGTGGGGATA CTCTCACCAA GGGTTGAAAG ATCTGCTTGG 
GTTCAACAAC CTCCAAATAG GACTGAAGTC TATGCAAGAC ATGCTGGGCA TTTCGGCCAA 
CATATAGAGT CACCCCACCA AATGCACCAC ACACCCCAAC ACCACCAGAA TCTCACACAT 
GCAACACACC AACACTCATC TCAAATCCCA CAACCTCAGC AGGCAGCTCC AATGGCAAGA 
CGTTTGCCTT CTTGCCAGAG TCTAGATGAG GAGCCGATTG GCTATCGTAG CTACAGTCCC 
TCATTCAATA GGAAGACTGG CCGTATCTTG CAGCAGGCCC AGTCATTTAG AGATCCCTCT 
TACACAGGTC CACACCTTTG GACTCCAACA TCCAAAACCA GTCCCCCAGA GGGGCATCCA 
CCCTCAGCCA CTGCTTCCCC AGCCCCACCA ACCACTTCCT CTTCAACTAC GATTTCCACA 
CCATCTGAAG GACAAGACAG AACTTACCGA CCCACTAATC ATGACAGAGG GGCAGGGGAA 
GCGACAAGTG AAGCCCAGAC ACAGGAAGTG GTTCTGAGGC AGAAACCACC TACCGGCCGA 
CGAGGTGCAA ACGCCCTTCG ACACCCACAT TATGCAATAG CACTTGACTC CAGTGACCCT 
CTACTCTTTA CCACTGACCC TACGGAGCCC ACTGCCAAGG CAGAAAGTGC TATTTCTCAT 
CGCCGCACAG ATGTTAACCT TCCTCCACTA TCTATTGAGG ATGACTCCCT TGCATCAATT 
CCATTCATCG ATGAGCCGAC CAGTCCAAGT GCAGATCTGC GGGCACGTCA TGTCCCCGCC 
TCCTCCGTCG TGTCCAGTGG CGGTATAAAC TCCGCCCCTG CTGTGATCAC AAGCCCCGCC 
TCCCCAACCT TCACCTTTCC CCTCAACCGC CTTTTCTCCC ACAACTGCAG TGATCCTAAA 
TCTAATCGTC GCTCCTCGTA CCTGTTGGCC ATCACCACTG AACGCTCCAA GTCATGTGAT 
GAGGGGCTTA ACGCATATAG AGAAGAGGGC CGGGTCTTCT CGAGGCTTCC AAAAAGAGTC 
AAGAGCTTCT TTACTGAAGG GTCTCTGGAC AGTCTGGGTG CTGCTGAAGA GGCTCGCTCT 
AAACGACACT CTACCTCTGA GCTGGGCAAC ATCAGCTACA GTGACGTGAA GAGAGAAGGC 
TGGCTCCACT TCAAACAGAT CCACACGGAG AAGGGCAAGA AGGTTGGCAG TGCAATCTGG 
CCATGGAGAC GGGTGTTCTC TGTGCTTAGG ACCCATTCTC TGTTTCTTTA TAAAGACAAG 
CGAGAGGCAG TGCTTAAAGG GGCGCAATTA GGGGGCGGGG CTGAAGACGA GCAGCCAATC 
AGCATCCGGG GCTGCCTTGT GGATATAGCA TACAGTGAAA CGAAGCGCAA GCACGCGCTG 
AGATTGACCA CGCAGGACTT CTGCGAGTAC CTGCTGCAGG CGGAGGACAG AGAGGACATG 
CTGGAGTGGA TCCGAGTCAT CAGGGACAAC AGCAAGACCG ACAACGAGGA ATTGGGTTTC 
TCACGACAAG CTCTCATCAA TAAAAAGCTT AACGACTACA GGAAGCAAAG TCCAACGGGT 
AACAAACCAG ACTCCTCACC CAGAGTGCAT CGAATGACCT TCCTCCTCAG CAAGCCAGAC 
AACAGCGCAC CTCCACGCTC CCCAAAACAC GAGGCCAAAG ATGAGAGCAG CCCCCCAAAA 
TCTCCCTGGG GTATAAACAT AATGAAGAAG AGCAAGAAGG CCGGTCCTAA AGCTTTTGGC 
GTCCGTCTAG AGGACTGCCA GCCTGCAGTA AACAACAAGT TCATTCCTCA GATTGTGGAG 
ATCTGCTGTG GGCTGGTGGA GGACATGGGT CTGGAGTATA CAGGGATCTA CAGAGTGCCA 
GGAAACAATG CTGTAGTCTC CAGTCTGCAG GAGCAGCTCA ACAAAGGAGT AGACATTAAC 
ATCACTGAGG AGAAATGGCA GGACCTGAAT GTGGTGAGCA GCCTGCTGAA GTCCTTCTTT 
CGTAAACTCC CTGAACCCCT CTTCACTGAT GATAAATATA ATGACTTTAT CGACGCCAAT 
CGGATGGAGA ACGCCAGCGA TAGGCTGAGG ACAATGAAGA AATTGATTCG GGACCTACCA 
GATCATTACT TTCACACTCT GAAATTTTTA GTTGGACACC TAAAGACGGT GGCTGACCAC 
TCTGAGAAAA ACAAAATGGA GCCGCGTAAT TTGGCTCTGG TGTTTGGCCC CACTCTTGTT 
CGAACTTCAG AGGACAACAT GACAGACATG GTCACCCACA TGCCAGACCG CTACAAGATT 
GTGGAGACAC TCATTCAGCA TTACACCTGG TTTTTCTGCG AAGAACAGGA TAAGGATGAA 
AAGACCCCAG TGGACACAGA GGATGTCCAG CCTGCCCCAA ACATTGATCA TCTGCTCTCC 
AACATTGGCC GCACAGGCAT GCTGGGTGAG GCTTCAGCTG AGCCTGTGGA ACAGCCACTG 
CGACTCAACC AACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap23a ( XM_009299680.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009297955.1 Danio rerio Rho GTPase activating protein 23a (arhgap23a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa48127
Clone ID Related Accession (Same CDS sequence)	XM_009299680.3 , XM_009299680.2
Accession Version	XM_009299680.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	rho GTPase-activating protein 23 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009299680.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGAG TTGCATTTTG CCTTGTTGGA ATACCTCCGT ATCCAGAAAG CGAAGCAAAA 
GGGCGGAGAG ATGGGCTCTC TTCATCCAGT GAGAACCCTC GGCCGGTGGT CCGGAGCGGG 
ACAGATGGGC TGGGCGTGTC CTGGCAGGGT CCACGCACGC TGGTGCTCCA AAAGAACTCT 
CAGGGCTTCG GCTTTACTCT GCGTCACTTC ATCGTTTACC CGCCAGAGTC CGCTCTGCAC 
ACCACTCTCA AGGATGAAGA GAACGGGAAT GGAAAGGGGC TTCAACGAAG TCGTCTGGAG 
CCCATGGACA CAATTTTTGT GAAGAATGTG AGGGAAAAAG GCCCCGCTCA CCTGGCTGGC 
CTGTGTACAG GAGACAGGCT GGTTAAAGTG AATGGGGAGA GCGTGCTGGG TAAGACGTAC 
TCGCAGGTCA TTGCACTGAT TCAGAACAGT GAGAGTGTAC TGGAGCTCTC CATTATGCCA 
AAGGATGAGG ACGTGCTTCA GCTGGCATAT TCCCATGATG CCTACCTAAG GGGGAACAAT 
CCATATACAG GAAAGGCCCA AAATCTTCCT CCACCACCCC CTGTTCTCTA CCCTCCTCGT 
ACAAAATCAC AGTCTCCTGC AGGGTTCAGC CCCTGCCCTC CTCCAGCTTC TTCACACATG 
GGGCAAAACC AGCTGGACAA CTGGAGCCGC TGGCCCGGCT CTTCTGCTGG TCCCTCTCCA 
CCCCTTGACA ACCGGACTGT GGCCTCCAGC AATGTTGTCT GGGCAGCCGA GGGCAGGAAT 
TGTGAGCCGG GTGGTGTTGG TCACAGTAGC CCTGCACACC GAACAGAGGA GATCCAGTAT 
GGAATGACTG ACAGAGGACA GCAGCCAACT GGACAAATGA GAGGACGCTC ATACTCATCT 
TCTTCCTCCT CAGGAGGAAT GATGAGCCCT TTGCATCATG GTCATCTTGC CAATCACAAC 
ATGGGTACTG CCATGGGGCA CACTGGAGGT CGTAAGGGAA GCCCACCTTG GGCCAGCCCT 
TCTCAGCCAA CATCAACACC TTCATCCAGC CGCAACGAAC GAACCCAACA AGCCCTTTCC 
AACTGGTACT ACAGCCAGGT CCCAGGCCGT GAGCGAGAAC GAGAACGAGA GCGGGAGCGA 
TCTAGTTCTC GCTCAATGCA CCCACGCCAC AGGAGTTTCT CACAAGACCG TCTGGGAGAG 
CTTGGGAAGG CCAGGCGTTC TCAACAAGCA GGTTTTCCCC ATAGTGCATC GCAAGACACG 
TTGCTGCTGC TTCAGCAGAG TGCTGCAGCA TATGCTGATC CTTACTGGAA CCATGGAGAC 
TGGCGGGGTG AGCATATGTA TGGTCAGCAC CCATCTGTGG TGGCCTATGG GCGAAAGTCC 
TCCGAAAATC TTCTAGCAAG CCGGTACGGA CACTCGGGAC GCTCACTGGA AAATCTCGAC 
AGAGCTGTGG GGATACTCTC ACCAAGGGTT GAAAGATCTG CTTGGGTTCA ACAACCTCCA 
AATAGGACTG AAGTCTATGC AAGACATGCT GGGCATTTCG GCCAACATAT AGAGTCACCC 
CACCAAATGC ACCACACACC CCAACACCAC CAGAATCTCA CACATGCAAC ACACCAACAC 
TCATCTCAAA TCCCACAACC TCAGCAGGCA GCTCCAATGG CAAGACGTTT GCCTTCTTGC 
CAGAGTCTAG ATGAGGAGCC GATTGGCTAT CGTAGCTACA GTCCCTCATT CAATAGGAAG 
ACTGGCCGTA TCTTGCAGCA GGCCCAGTCA TTTAGAGATC CCTCTTACAC AGGTCCACAC 
CTTTGGACTC CAACATCCAA AACCAGTCCC CCAGAGGGGC ATCCACCCTC AGCCACTGCT 
TCCCCAGCCC CACCAACCAC TTCCTCTTCA ACTACGATTT CCACACCATC TGAAGGACAA 
GACAGAACTT ACCGACCCAC TAATCATGAC AGAGGGGCAG GGGAAGCGAC AAGTGAAGCC 
CAGACACAGG AAGTGGTTCT GAGGCAGAAA CCACCTACCG GCCGACGAGG TGCAAACGCC 
CTTCGACACC CACATTATGC AATAGCACTT GACTCCAGTG ACCCTCTACT CTTTACCACT 
GACCCTACGG AGCCCACTGC CAAGGCAGAA AGTGCTATTT CTCATCGCCG CACAGATGTT 
AACCTTCCTC CACTATCTAT TGAGGATGAC TCCCTTGCAT CAATTCCATT CATCGATGAG 
CCGACCAGTC CAAGTGCAGA TCTGCGGGCA CGTCATGTCC CCGCCTCCTC CGTCGTGTCC 
AGTGGCGGTA TAAACTCCGC CCCTGCTGTG ATCACAAGCC CCGCCTCCCC AACCTTCACC 
TTTCCCCTCA ACCGCCTTTT CTCCCACAAC TGCAGTGATC CTAAATCTAA TCGTCGCTCC 
TCGTACCTGT TGGCCATCAC CACTGAACGC TCCAAGTCAT GTGATGAGGG GCTTAACGCA 
TATAGAGAAG AGGGCCGGGT CTTCTCGAGG CTTCCAAAAA GAGTCAAGAG CTTCTTTACT 
GAAGGGTCTC TGGACAGTCT GGGTGCTGCT GAAGAGGCTC GCTCTAAACG ACACTCTACC 
TCTGAGCTGG GCAACATCAG CTACAGTGAC GTGAAGAGAG AAGGCTGGCT CCACTTCAAA 
CAGATCCACA CGGAGAAGGG CAAGAAGGTT GGCAGTGCAA TCTGGCCATG GAGACGGGTG 
TTCTCTGTGC TTAGGACCCA TTCTCTGTTT CTTTATAAAG ACAAGCGAGA GGCAGTGCTT 
AAAGGGGCGC AATTAGGGGG CGGGGCTGAA GACGAGCAGC CAATCAGCAT CCGGGGCTGC 
CTTGTGGATA TAGCATACAG TGAAACGAAG CGCAAGCACG CGCTGAGATT GACCACGCAG 
GACTTCTGCG AGTACCTGCT GCAGGCGGAG GACAGAGAGG ACATGCTGGA GTGGATCCGA 
GTCATCAGGG ACAACAGCAA GACCGACAAC GAGGAATTGG GTTTCTCACG ACAAGCTCTC 
ATCAATAAAA AGCTTAACGA CTACAGGAAG CAAAGTCCAA CGGGTAACAA ACCAGACTCC 
TCACCCAGAG TGCATCGAAT GACCTTCCTC CTCAGCAAGC CAGACAACAG CGCACCTCCA 
CGCTCCCCAA AACACGAGGC CAAAGATGAG AGCAGCCCCC CAAAATCTCC CTGGGGTATA 
AACATAATGA AGAAGAGCAA GAAGGCCGGT CCTAAAGCTT TTGGCGTCCG TCTAGAGGAC 
TGCCAGCCTG CAGTAAACAA CAAGTTCATT CCTCAGATTG TGGAGATCTG CTGTGGGCTG 
GTGGAGGACA TGGGTCTGGA GTATACAGGG ATCTACAGAG TGCCAGGAAA CAATGCTGTA 
GTCTCCAGTC TGCAGGAGCA GCTCAACAAA GGAGTAGACA TTAACATCAC TGAGGAGAAA 
TGGCAGGACC TGAATGTGGT GAGCAGCCTG CTGAAGTCCT TCTTTCGTAA ACTCCCTGAA 
CCCCTCTTCA CTGATGATAA ATATAATGAC TTTATCGACG CCAATCGGAT GGAGAACGCC 
AGCGATAGGC TGAGGACAAT GAAGAAATTG ATTCGGGACC TACCAGATCA TTACTTTCAC 
ACTCTGAAAT TTTTAGTTGG ACACCTAAAG ACGGTGGCTG ACCACTCTGA GAAAAACAAA 
ATGGAGCCGC GTAATTTGGC TCTGGTGTTT GGCCCCACTC TTGTTCGAAC TTCAGAGGAC 
AACATGACAG ACATGGTCAC CCACATGCCA GACCGCTACA AGATTGTGGA GACACTCATT 
CAGCATTACA CCTGGTTTTT CTGCGAAGAA CAGGATAAGG ATGAAAAGAC CCCAGTGGAC 
ACAGAGGATG TCCAGCCTGC CCCAAACATT GATCATCTGC TCTCCAACAT TGGCCGCACA 
GGCATGCTGG GTGAGGCTTC AGACTCAACC AACAGTGATT CTGCAAAATC TAAGGGGTCA 
TGGGGATCAA AACGTGACTT TACTGCCAAG GATATCCTTA CATTATCAAT TATGTCGGCT 
GTGACCCGCA AACGGCGGAA GCGGCACAAC GGCCGTCTCC TCGGCAGCAG CACAGACGAC 
GATTCTGAGC ATGAACCCAT TAAATCCTGC ATCTCCGAGA AAGACGATCT GCAAGAGATG 
GGGCAGGTTG CATCCTTCTG TGCCCCACGA GCAGAGGGAG AGGAGGATGA CGATGAAGAG 
GAGGAAGATG ATGAAGAGGA GGAGGAGGGG GCTGAGAGAC AGAAGGCACA AGAGCAGAAG 
GAAACAGTAG TCCCCAGCAT TTCCTCAACA GAGGAAGTGA CATCAACAGT GCTGCTGAGC 
GAAGAGGAAG AGCAGAAGTC TGAAGTGAAA GGAGGACGCA GCTGGAGATT AGATGATGCT 
CGGTCTATTG TGTCAGGCTA CTCTACTCTG TCCACTTTGG GGCGGAGCTT AGCTTCAGAG 
GGGCGGGGCG AGGATGCAGA TGATGAGCAT AGTGAACTAG TGAGTGAAAC TGATAATGAA 
AGCGGGTTTG CCTCACGCTC CCTCACCCAA GAAAGGCCAG AAAAACCAAA CCGTTCCAAC 
CAGAACTCTC ATGCGTCTCC TGAACCAACC GCACCACAGA GTTTCCTCTA TGCGCACTGC 
AAATCACCTA CACCCTCTGG CTCCGCCACT GCTCCATCTC CCCCTTCACA ACACATCACA 
ACCAACCCAG AGGACAGCGC AGCACGCTCG TCTACTCCCT CAACCTCCTC TTACTCCTCA 
TCCGCCTCAC AACGGCTCCA CAACCGCTCT TTCAACTCCC ACCGCCTCAT ACAGTGCGAC 
ACCCTTGCAC GCCGTCGGAC AAAGGCGGAC AAAGCCAAAG CTCCCTCAGT GGACCTTTCC 
GAACTCTCGA GCTCCTCCGC CGCAGAGGAG GACCGCCAGT CAGGTGCCAA TCAAATCCCT 
GACTCCTCTC CGTCTCCTTC TTCAAAAGCC AAAGGGTTTA GCATAAGAAA CGAGTGTACG 
CCAATATCCA TGGTTGGTCC CGGAAGTAAG GGGTGTGTGT TGTCCCCCGG GCAAGGATCC 
CTGGCGGATC AGGTACGTGC TCGTCTAATG GGCTCGGCGG ATGACCTGTG CAGCGTTGGC 
CTGAGAAAGC AGCTCTCGCC GGAGACGCGC CGGAAGAGAC GCGCTTGGAG GAGACACACA 
GTGGTCGTGT CCCCTACGGA TTGTTCAGAC AAAAAGCCAA CGGTCCAAAG CACCAAACCT 
CTAGCTTCAC CCTCCCCTGT TGAAAAGCAG GATGTCAGAG AGTCCCACCC AGAGTGCTCA 
GAAACTGAAG CCCCTACTGC ACGTCGGGCC GTGCCTACCT CGCGCTTTCG TGATTTCTTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297955.1
CDS	234..5576
Translation	MNGVAFCLVGIPPYPESEAKGRRDGLSSSSENPRPVVRSGTDGLGVSWQGPRTLVLQKNSQGFGFTLRHFIVYPPESALHTTLKDEENGNGKGLQRSRLEPMDTIFVKNVREKGPAHLAGLCTGDRLVKVNGESVLGKTYSQVIALIQNSESVLELSIMPKDEDVLQLAYSHDAYLRGNNPYTGKAQNLPPPPPVLYPPRTKSQSPAGFSPCPPPASSHMGQNQLDNWSRWPGSSAGPSPPLDNRTVASSNVVWAAEGRNCEPGGVGHSSPAHRTEEIQYGMTDRGQQPTGQMRGRSYSSSSSSGGMMSPLHHGHLANHNMGTAMGHTGGRKGSPPWASPSQPTSTPSSSRNERTQQALSNWYYSQVPGRERERERERERSSSRSMHPRHRSFSQDRLGELGKARRSQQAGFPHSASQDTLLLLQQSAAAYADPYWNHGDWRGEHMYGQHPSVVAYGRKSSENLLASRYGHSGRSLENLDRAVGILSPRVERSAWVQQPPNRTEVYARHAGHFGQHIESPHQMHHTPQHHQNLTHATHQHSSQIPQPQQAAPMARRLPSCQSLDEEPIGYRSYSPSFNRKTGRILQQAQSFRDPSYTGPHLWTPTSKTSPPEGHPPSATASPAPPTTSSSTTISTPSEGQDRTYRPTNHDRGAGEATSEAQTQEVVLRQKPPTGRRGANALRHPHYAIALDSSDPLLFTTDPTEPTAKAESAISHRRTDVNLPPLSIEDDSLASIPFIDEPTSPSADLRARHVPASSVVSSGGINSAPAVITSPASPTFTFPLNRLFSHNCSDPKSNRRSSYLLAITTERSKSCDEGLNAYREEGRVFSRLPKRVKSFFTEGSLDSLGAAEEARSKRHSTSELGNISYSDVKREGWLHFKQIHTEKGKKVGSAIWPWRRVFSVLRTHSLFLYKDKREAVLKGAQLGGGAEDEQPISIRGCLVDIAYSETKRKHALRLTTQDFCEYLLQAEDREDMLEWIRVIRDNSKTDNEELGFSRQALINKKLNDYRKQSPTGNKPDSSPRVHRMTFLLSKPDNSAPPRSPKHEAKDESSPPKSPWGINIMKKSKKAGPKAFGVRLEDCQPAVNNKFIPQIVEICCGLVEDMGLEYTGIYRVPGNNAVVSSLQEQLNKGVDINITEEKWQDLNVVSSLLKSFFRKLPEPLFTDDKYNDFIDANRMENASDRLRTMKKLIRDLPDHYFHTLKFLVGHLKTVADHSEKNKMEPRNLALVFGPTLVRTSEDNMTDMVTHMPDRYKIVETLIQHYTWFFCEEQDKDEKTPVDTEDVQPAPNIDHLLSNIGRTGMLGEASDSTNSDSAKSKGSWGSKRDFTAKDILTLSIMSAVTRKRRKRHNGRLLGSSTDDDSEHEPIKSCISEKDDLQEMGQVASFCAPRAEGEEDDDEEEEDDEEEEEGAERQKAQEQKETVVPSISSTEEVTSTVLLSEEEEQKSEVKGGRSWRLDDARSIVSGYSTLSTLGRSLASEGRGEDADDEHSELVSETDNESGFASRSLTQERPEKPNRSNQNSHASPEPTAPQSFLYAHCKSPTPSGSATAPSPPSQHITTNPEDSAARSSTPSTSSYSSSASQRLHNRSFNSHRLIQCDTLARRRTKADKAKAPSVDLSELSSSSAAEEDRQSGANQIPDSSPSPSSKAKGFSIRNECTPISMVGPGSKGCVLSPGQGSLADQVRARLMGSADDLCSVGLRKQLSPETRRKRRAWRRHTVVVSPTDCSDKKPTVQSTKPLASPSPVEKQDVRESHPECSETEAPTARRAVPTSRFRDFL

Target ORF information:

RefSeq Version	XM_009299680.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Rho GTPase activating protein 23a (arhgap23a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299680.3

ORF Insert Sequence:

ATGAATGGAG TTGCATTTTG CCTTGTTGGA ATACCTCCGT ATCCAGAAAG CGAAGCAAAA 
GGGCGGAGAG ATGGGCTCTC TTCATCCAGT GAGAACCCTC GGCCGGTGGT CCGGAGCGGG 
ACAGATGGGC TGGGCGTGTC CTGGCAGGGT CCACGCACGC TGGTGCTCCA AAAGAACTCT 
CAGGGCTTCG GCTTTACTCT GCGTCACTTC ATCGTTTACC CGCCAGAGTC CGCTCTGCAC 
ACCACTCTCA AGGATGAAGA GAACGGGAAT GGAAAGGGGC TTCAACGAAG TCGTCTGGAG 
CCCATGGACA CAATTTTTGT GAAGAATGTG AGGGAAAAAG GCCCCGCTCA CCTGGCTGGC 
CTGTGTACAG GAGACAGGCT GGTTAAAGTG AATGGGGAGA GCGTGCTGGG TAAGACGTAC 
TCGCAGGTCA TTGCACTGAT TCAGAACAGT GAGAGTGTAC TGGAGCTCTC CATTATGCCA 
AAGGATGAGG ACGTGCTTCA GCTGGCATAT TCCCATGATG CCTACCTAAG GGGGAACAAT 
CCATATACAG GAAAGGCCCA AAATCTTCCT CCACCACCCC CTGTTCTCTA CCCTCCTCGT 
ACAAAATCAC AGTCTCCTGC AGGGTTCAGC CCCTGCCCTC CTCCAGCTTC TTCACACATG 
GGGCAAAACC AGCTGGACAA CTGGAGCCGC TGGCCCGGCT CTTCTGCTGG TCCCTCTCCA 
CCCCTTGACA ACCGGACTGT GGCCTCCAGC AATGTTGTCT GGGCAGCCGA GGGCAGGAAT 
TGTGAGCCGG GTGGTGTTGG TCACAGTAGC CCTGCACACC GAACAGAGGA GATCCAGTAT 
GGAATGACTG ACAGAGGACA GCAGCCAACT GGACAAATGA GAGGACGCTC ATACTCATCT 
TCTTCCTCCT CAGGAGGAAT GATGAGCCCT TTGCATCATG GTCATCTTGC CAATCACAAC 
ATGGGTACTG CCATGGGGCA CACTGGAGGT CGTAAGGGAA GCCCACCTTG GGCCAGCCCT 
TCTCAGCCAA CATCAACACC TTCATCCAGC CGCAACGAAC GAACCCAACA AGCCCTTTCC 
AACTGGTACT ACAGCCAGGT CCCAGGCCGT GAGCGAGAAC GAGAACGAGA GCGGGAGCGA 
TCTAGTTCTC GCTCAATGCA CCCACGCCAC AGGAGTTTCT CACAAGACCG TCTGGGAGAG 
CTTGGGAAGG CCAGGCGTTC TCAACAAGCA GGTTTTCCCC ATAGTGCATC GCAAGACACG 
TTGCTGCTGC TTCAGCAGAG TGCTGCAGCA TATGCTGATC CTTACTGGAA CCATGGAGAC 
TGGCGGGGTG AGCATATGTA TGGTCAGCAC CCATCTGTGG TGGCCTATGG GCGAAAGTCC 
TCCGAAAATC TTCTAGCAAG CCGGTACGGA CACTCGGGAC GCTCACTGGA AAATCTCGAC 
AGAGCTGTGG GGATACTCTC ACCAAGGGTT GAAAGATCTG CTTGGGTTCA ACAACCTCCA 
AATAGGACTG AAGTCTATGC AAGACATGCT GGGCATTTCG GCCAACATAT AGAGTCACCC 
CACCAAATGC ACCACACACC CCAACACCAC CAGAATCTCA CACATGCAAC ACACCAACAC 
TCATCTCAAA TCCCACAACC TCAGCAGGCA GCTCCAATGG CAAGACGTTT GCCTTCTTGC 
CAGAGTCTAG ATGAGGAGCC GATTGGCTAT CGTAGCTACA GTCCCTCATT CAATAGGAAG 
ACTGGCCGTA TCTTGCAGCA GGCCCAGTCA TTTAGAGATC CCTCTTACAC AGGTCCACAC 
CTTTGGACTC CAACATCCAA AACCAGTCCC CCAGAGGGGC ATCCACCCTC AGCCACTGCT 
TCCCCAGCCC CACCAACCAC TTCCTCTTCA ACTACGATTT CCACACCATC TGAAGGACAA 
GACAGAACTT ACCGACCCAC TAATCATGAC AGAGGGGCAG GGGAAGCGAC AAGTGAAGCC 
CAGACACAGG AAGTGGTTCT GAGGCAGAAA CCACCTACCG GCCGACGAGG TGCAAACGCC 
CTTCGACACC CACATTATGC AATAGCACTT GACTCCAGTG ACCCTCTACT CTTTACCACT 
GACCCTACGG AGCCCACTGC CAAGGCAGAA AGTGCTATTT CTCATCGCCG CACAGATGTT 
AACCTTCCTC CACTATCTAT TGAGGATGAC TCCCTTGCAT CAATTCCATT CATCGATGAG 
CCGACCAGTC CAAGTGCAGA TCTGCGGGCA CGTCATGTCC CCGCCTCCTC CGTCGTGTCC 
AGTGGCGGTA TAAACTCCGC CCCTGCTGTG ATCACAAGCC CCGCCTCCCC AACCTTCACC 
TTTCCCCTCA ACCGCCTTTT CTCCCACAAC TGCAGTGATC CTAAATCTAA TCGTCGCTCC 
TCGTACCTGT TGGCCATCAC CACTGAACGC TCCAAGTCAT GTGATGAGGG GCTTAACGCA 
TATAGAGAAG AGGGCCGGGT CTTCTCGAGG CTTCCAAAAA GAGTCAAGAG CTTCTTTACT 
GAAGGGTCTC TGGACAGTCT GGGTGCTGCT GAAGAGGCTC GCTCTAAACG ACACTCTACC 
TCTGAGCTGG GCAACATCAG CTACAGTGAC GTGAAGAGAG AAGGCTGGCT CCACTTCAAA 
CAGATCCACA CGGAGAAGGG CAAGAAGGTT GGCAGTGCAA TCTGGCCATG GAGACGGGTG 
TTCTCTGTGC TTAGGACCCA TTCTCTGTTT CTTTATAAAG ACAAGCGAGA GGCAGTGCTT 
AAAGGGGCGC AATTAGGGGG CGGGGCTGAA GACGAGCAGC CAATCAGCAT CCGGGGCTGC 
CTTGTGGATA TAGCATACAG TGAAACGAAG CGCAAGCACG CGCTGAGATT GACCACGCAG 
GACTTCTGCG AGTACCTGCT GCAGGCGGAG GACAGAGAGG ACATGCTGGA GTGGATCCGA 
GTCATCAGGG ACAACAGCAA GACCGACAAC GAGGAATTGG GTTTCTCACG ACAAGCTCTC 
ATCAATAAAA AGCTTAACGA CTACAGGAAG CAAAGTCCAA CGGGTAACAA ACCAGACTCC 
TCACCCAGAG TGCATCGAAT GACCTTCCTC CTCAGCAAGC CAGACAACAG CGCACCTCCA 
CGCTCCCCAA AACACGAGGC CAAAGATGAG AGCAGCCCCC CAAAATCTCC CTGGGGTATA 
AACATAATGA AGAAGAGCAA GAAGGCCGGT CCTAAAGCTT TTGGCGTCCG TCTAGAGGAC 
TGCCAGCCTG CAGTAAACAA CAAGTTCATT CCTCAGATTG TGGAGATCTG CTGTGGGCTG 
GTGGAGGACA TGGGTCTGGA GTATACAGGG ATCTACAGAG TGCCAGGAAA CAATGCTGTA 
GTCTCCAGTC TGCAGGAGCA GCTCAACAAA GGAGTAGACA TTAACATCAC TGAGGAGAAA 
TGGCAGGACC TGAATGTGGT GAGCAGCCTG CTGAAGTCCT TCTTTCGTAA ACTCCCTGAA 
CCCCTCTTCA CTGATGATAA ATATAATGAC TTTATCGACG CCAATCGGAT GGAGAACGCC 
AGCGATAGGC TGAGGACAAT GAAGAAATTG ATTCGGGACC TACCAGATCA TTACTTTCAC 
ACTCTGAAAT TTTTAGTTGG ACACCTAAAG ACGGTGGCTG ACCACTCTGA GAAAAACAAA 
ATGGAGCCGC GTAATTTGGC TCTGGTGTTT GGCCCCACTC TTGTTCGAAC TTCAGAGGAC 
AACATGACAG ACATGGTCAC CCACATGCCA GACCGCTACA AGATTGTGGA GACACTCATT 
CAGCATTACA CCTGGTTTTT CTGCGAAGAA CAGGATAAGG ATGAAAAGAC CCCAGTGGAC 
ACAGAGGATG TCCAGCCTGC CCCAAACATT GATCATCTGC TCTCCAACAT TGGCCGCACA 
GGCATGCTGG GTGAGGCTTC AGACTCAACC AACAGTGATT CTGCAAAATC TAAGGGGTCA 
TGGGGATCAA AACGTGACTT TACTGCCAAG GATATCCTTA CATTATCAAT TATGTCGGCT 
GTGACCCGCA AACGGCGGAA GCGGCACAAC GGCCGTCTCC TCGGCAGCAG CACAGACGAC 
GATTCTGAGC ATGAACCCAT TAAATCCTGC ATCTCCGAGA AAGACGATCT GCAAGAGATG 
GGGCAGGTTG CATCCTTCTG TGCCCCACGA GCAGAGGGAG AGGAGGATGA CGATGAAGAG 
GAGGAAGATG ATGAAGAGGA GGAGGAGGGG GCTGAGAGAC AGAAGGCACA AGAGCAGAAG 
GAAACAGTAG TCCCCAGCAT TTCCTCAACA GAGGAAGTGA CATCAACAGT GCTGCTGAGC 
GAAGAGGAAG AGCAGAAGTC TGAAGTGAAA GGAGGACGCA GCTGGAGATT AGATGATGCT 
CGGTCTATTG TGTCAGGCTA CTCTACTCTG TCCACTTTGG GGCGGAGCTT AGCTTCAGAG 
GGGCGGGGCG AGGATGCAGA TGATGAGCAT AGTGAACTAG TGAGTGAAAC TGATAATGAA 
AGCGGGTTTG CCTCACGCTC CCTCACCCAA GAAAGGCCAG AAAAACCAAA CCGTTCCAAC 
CAGAACTCTC ATGCGTCTCC TGAACCAACC GCACCACAGA GTTTCCTCTA TGCGCACTGC 
AAATCACCTA CACCCTCTGG CTCCGCCACT GCTCCATCTC CCCCTTCACA ACACATCACA 
ACCAACCCAG AGGACAGCGC AGCACGCTCG TCTACTCCCT CAACCTCCTC TTACTCCTCA 
TCCGCCTCAC AACGGCTCCA CAACCGCTCT TTCAACTCCC ACCGCCTCAT ACAGTGCGAC 
ACCCTTGCAC GCCGTCGGAC AAAGGCGGAC AAAGCCAAAG CTCCCTCAGT GGACCTTTCC 
GAACTCTCGA GCTCCTCCGC CGCAGAGGAG GACCGCCAGT CAGGTGCCAA TCAAATCCCT 
GACTCCTCTC CGTCTCCTTC TTCAAAAGCC AAAGGGTTTA GCATAAGAAA CGAGTGTACG 
CCAATATCCA TGGTTGGTCC CGGAAGTAAG GGGTGTGTGT TGTCCCCCGG GCAAGGATCC 
CTGGCGGATC AGGTACGTGC TCGTCTAATG GGCTCGGCGG ATGACCTGTG CAGCGTTGGC 
CTGAGAAAGC AGCTCTCGCC GGAGACGCGC CGGAAGAGAC GCGCTTGGAG GAGACACACA 
GTGGTCGTGT CCCCTACGGA TTGTTCAGAC AAAAAGCCAA CGGTCCAAAG CACCAAACCT 
CTAGCTTCAC CCTCCCCTGT TGAAAAGCAG GATGTCAGAG AGTCCCACCC AGAGTGCTCA 
GAAACTGAAG CCCCTACTGC ACGTCGGGCC GTGCCTACCT CGCGCTTTCG TGATTTCTTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap23a ( XM_009299680.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009297955.1 Danio rerio Rho GTPase activating protein 23a (arhgap23a), transcript variant X3, mRNA.

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CloneID	ODa48127
Clone ID Related Accession (Same CDS sequence)	XM_009299680.3 , XM_009299680.2
Accession Version	XM_009299680.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	rho GTPase-activating protein 23 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299680.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGAG TTGCATTTTG CCTTGTTGGA ATACCTCCGT ATCCAGAAAG CGAAGCAAAA 
GGGCGGAGAG ATGGGCTCTC TTCATCCAGT GAGAACCCTC GGCCGGTGGT CCGGAGCGGG 
ACAGATGGGC TGGGCGTGTC CTGGCAGGGT CCACGCACGC TGGTGCTCCA AAAGAACTCT 
CAGGGCTTCG GCTTTACTCT GCGTCACTTC ATCGTTTACC CGCCAGAGTC CGCTCTGCAC 
ACCACTCTCA AGGATGAAGA GAACGGGAAT GGAAAGGGGC TTCAACGAAG TCGTCTGGAG 
CCCATGGACA CAATTTTTGT GAAGAATGTG AGGGAAAAAG GCCCCGCTCA CCTGGCTGGC 
CTGTGTACAG GAGACAGGCT GGTTAAAGTG AATGGGGAGA GCGTGCTGGG TAAGACGTAC 
TCGCAGGTCA TTGCACTGAT TCAGAACAGT GAGAGTGTAC TGGAGCTCTC CATTATGCCA 
AAGGATGAGG ACGTGCTTCA GCTGGCATAT TCCCATGATG CCTACCTAAG GGGGAACAAT 
CCATATACAG GAAAGGCCCA AAATCTTCCT CCACCACCCC CTGTTCTCTA CCCTCCTCGT 
ACAAAATCAC AGTCTCCTGC AGGGTTCAGC CCCTGCCCTC CTCCAGCTTC TTCACACATG 
GGGCAAAACC AGCTGGACAA CTGGAGCCGC TGGCCCGGCT CTTCTGCTGG TCCCTCTCCA 
CCCCTTGACA ACCGGACTGT GGCCTCCAGC AATGTTGTCT GGGCAGCCGA GGGCAGGAAT 
TGTGAGCCGG GTGGTGTTGG TCACAGTAGC CCTGCACACC GAACAGAGGA GATCCAGTAT 
GGAATGACTG ACAGAGGACA GCAGCCAACT GGACAAATGA GAGGACGCTC ATACTCATCT 
TCTTCCTCCT CAGGAGGAAT GATGAGCCCT TTGCATCATG GTCATCTTGC CAATCACAAC 
ATGGGTACTG CCATGGGGCA CACTGGAGGT CGTAAGGGAA GCCCACCTTG GGCCAGCCCT 
TCTCAGCCAA CATCAACACC TTCATCCAGC CGCAACGAAC GAACCCAACA AGCCCTTTCC 
AACTGGTACT ACAGCCAGGT CCCAGGCCGT GAGCGAGAAC GAGAACGAGA GCGGGAGCGA 
TCTAGTTCTC GCTCAATGCA CCCACGCCAC AGGAGTTTCT CACAAGACCG TCTGGGAGAG 
CTTGGGAAGG CCAGGCGTTC TCAACAAGCA GGTTTTCCCC ATAGTGCATC GCAAGACACG 
TTGCTGCTGC TTCAGCAGAG TGCTGCAGCA TATGCTGATC CTTACTGGAA CCATGGAGAC 
TGGCGGGGTG AGCATATGTA TGGTCAGCAC CCATCTGTGG TGGCCTATGG GCGAAAGTCC 
TCCGAAAATC TTCTAGCAAG CCGGTACGGA CACTCGGGAC GCTCACTGGA AAATCTCGAC 
AGAGCTGTGG GGATACTCTC ACCAAGGGTT GAAAGATCTG CTTGGGTTCA ACAACCTCCA 
AATAGGACTG AAGTCTATGC AAGACATGCT GGGCATTTCG GCCAACATAT AGAGTCACCC 
CACCAAATGC ACCACACACC CCAACACCAC CAGAATCTCA CACATGCAAC ACACCAACAC 
TCATCTCAAA TCCCACAACC TCAGCAGGCA GCTCCAATGG CAAGACGTTT GCCTTCTTGC 
CAGAGTCTAG ATGAGGAGCC GATTGGCTAT CGTAGCTACA GTCCCTCATT CAATAGGAAG 
ACTGGCCGTA TCTTGCAGCA GGCCCAGTCA TTTAGAGATC CCTCTTACAC AGGTCCACAC 
CTTTGGACTC CAACATCCAA AACCAGTCCC CCAGAGGGGC ATCCACCCTC AGCCACTGCT 
TCCCCAGCCC CACCAACCAC TTCCTCTTCA ACTACGATTT CCACACCATC TGAAGGACAA 
GACAGAACTT ACCGACCCAC TAATCATGAC AGAGGGGCAG GGGAAGCGAC AAGTGAAGCC 
CAGACACAGG AAGTGGTTCT GAGGCAGAAA CCACCTACCG GCCGACGAGG TGCAAACGCC 
CTTCGACACC CACATTATGC AATAGCACTT GACTCCAGTG ACCCTCTACT CTTTACCACT 
GACCCTACGG AGCCCACTGC CAAGGCAGAA AGTGCTATTT CTCATCGCCG CACAGATGTT 
AACCTTCCTC CACTATCTAT TGAGGATGAC TCCCTTGCAT CAATTCCATT CATCGATGAG 
CCGACCAGTC CAAGTGCAGA TCTGCGGGCA CGTCATGTCC CCGCCTCCTC CGTCGTGTCC 
AGTGGCGGTA TAAACTCCGC CCCTGCTGTG ATCACAAGCC CCGCCTCCCC AACCTTCACC 
TTTCCCCTCA ACCGCCTTTT CTCCCACAAC TGCAGTGATC CTAAATCTAA TCGTCGCTCC 
TCGTACCTGT TGGCCATCAC CACTGAACGC TCCAAGTCAT GTGATGAGGG GCTTAACGCA 
TATAGAGAAG AGGGCCGGGT CTTCTCGAGG CTTCCAAAAA GAGTCAAGAG CTTCTTTACT 
GAAGGGTCTC TGGACAGTCT GGGTGCTGCT GAAGAGGCTC GCTCTAAACG ACACTCTACC 
TCTGAGCTGG GCAACATCAG CTACAGTGAC GTGAAGAGAG AAGGCTGGCT CCACTTCAAA 
CAGATCCACA CGGAGAAGGG CAAGAAGGTT GGCAGTGCAA TCTGGCCATG GAGACGGGTG 
TTCTCTGTGC TTAGGACCCA TTCTCTGTTT CTTTATAAAG ACAAGCGAGA GGCAGTGCTT 
AAAGGGGCGC AATTAGGGGG CGGGGCTGAA GACGAGCAGC CAATCAGCAT CCGGGGCTGC 
CTTGTGGATA TAGCATACAG TGAAACGAAG CGCAAGCACG CGCTGAGATT GACCACGCAG 
GACTTCTGCG AGTACCTGCT GCAGGCGGAG GACAGAGAGG ACATGCTGGA GTGGATCCGA 
GTCATCAGGG ACAACAGCAA GACCGACAAC GAGGAATTGG GTTTCTCACG ACAAGCTCTC 
ATCAATAAAA AGCTTAACGA CTACAGGAAG CAAAGTCCAA CGGGTAACAA ACCAGACTCC 
TCACCCAGAG TGCATCGAAT GACCTTCCTC CTCAGCAAGC CAGACAACAG CGCACCTCCA 
CGCTCCCCAA AACACGAGGC CAAAGATGAG AGCAGCCCCC CAAAATCTCC CTGGGGTATA 
AACATAATGA AGAAGAGCAA GAAGGCCGGT CCTAAAGCTT TTGGCGTCCG TCTAGAGGAC 
TGCCAGCCTG CAGTAAACAA CAAGTTCATT CCTCAGATTG TGGAGATCTG CTGTGGGCTG 
GTGGAGGACA TGGGTCTGGA GTATACAGGG ATCTACAGAG TGCCAGGAAA CAATGCTGTA 
GTCTCCAGTC TGCAGGAGCA GCTCAACAAA GGAGTAGACA TTAACATCAC TGAGGAGAAA 
TGGCAGGACC TGAATGTGGT GAGCAGCCTG CTGAAGTCCT TCTTTCGTAA ACTCCCTGAA 
CCCCTCTTCA CTGATGATAA ATATAATGAC TTTATCGACG CCAATCGGAT GGAGAACGCC 
AGCGATAGGC TGAGGACAAT GAAGAAATTG ATTCGGGACC TACCAGATCA TTACTTTCAC 
ACTCTGAAAT TTTTAGTTGG ACACCTAAAG ACGGTGGCTG ACCACTCTGA GAAAAACAAA 
ATGGAGCCGC GTAATTTGGC TCTGGTGTTT GGCCCCACTC TTGTTCGAAC TTCAGAGGAC 
AACATGACAG ACATGGTCAC CCACATGCCA GACCGCTACA AGATTGTGGA GACACTCATT 
CAGCATTACA CCTGGTTTTT CTGCGAAGAA CAGGATAAGG ATGAAAAGAC CCCAGTGGAC 
ACAGAGGATG TCCAGCCTGC CCCAAACATT GATCATCTGC TCTCCAACAT TGGCCGCACA 
GGCATGCTGG GTGAGGCTTC AGACTCAACC AACAGTGATT CTGCAAAATC TAAGGGGTCA 
TGGGGATCAA AACGTGACTT TACTGCCAAG GATATCCTTA CATTATCAAT TATGTCGGCT 
GTGACCCGCA AACGGCGGAA GCGGCACAAC GGCCGTCTCC TCGGCAGCAG CACAGACGAC 
GATTCTGAGC ATGAACCCAT TAAATCCTGC ATCTCCGAGA AAGACGATCT GCAAGAGATG 
GGGCAGGTTG CATCCTTCTG TGCCCCACGA GCAGAGGGAG AGGAGGATGA CGATGAAGAG 
GAGGAAGATG ATGAAGAGGA GGAGGAGGGG GCTGAGAGAC AGAAGGCACA AGAGCAGAAG 
GAAACAGTAG TCCCCAGCAT TTCCTCAACA GAGGAAGTGA CATCAACAGT GCTGCTGAGC 
GAAGAGGAAG AGCAGAAGTC TGAAGTGAAA GGAGGACGCA GCTGGAGATT AGATGATGCT 
CGGTCTATTG TGTCAGGCTA CTCTACTCTG TCCACTTTGG GGCGGAGCTT AGCTTCAGAG 
GGGCGGGGCG AGGATGCAGA TGATGAGCAT AGTGAACTAG TGAGTGAAAC TGATAATGAA 
AGCGGGTTTG CCTCACGCTC CCTCACCCAA GAAAGGCCAG AAAAACCAAA CCGTTCCAAC 
CAGAACTCTC ATGCGTCTCC TGAACCAACC GCACCACAGA GTTTCCTCTA TGCGCACTGC 
AAATCACCTA CACCCTCTGG CTCCGCCACT GCTCCATCTC CCCCTTCACA ACACATCACA 
ACCAACCCAG AGGACAGCGC AGCACGCTCG TCTACTCCCT CAACCTCCTC TTACTCCTCA 
TCCGCCTCAC AACGGCTCCA CAACCGCTCT TTCAACTCCC ACCGCCTCAT ACAGTGCGAC 
ACCCTTGCAC GCCGTCGGAC AAAGGCGGAC AAAGCCAAAG CTCCCTCAGT GGACCTTTCC 
GAACTCTCGA GCTCCTCCGC CGCAGAGGAG GACCGCCAGT CAGGTGCCAA TCAAATCCCT 
GACTCCTCTC CGTCTCCTTC TTCAAAAGCC AAAGGGTTTA GCATAAGAAA CGAGTGTACG 
CCAATATCCA TGGTTGGTCC CGGAAGTAAG GGGTGTGTGT TGTCCCCCGG GCAAGGATCC 
CTGGCGGATC AGGTACGTGC TCGTCTAATG GGCTCGGCGG ATGACCTGTG CAGCGTTGGC 
CTGAGAAAGC AGCTCTCGCC GGAGACGCGC CGGAAGAGAC GCGCTTGGAG GAGACACACA 
GTGGTCGTGT CCCCTACGGA TTGTTCAGAC AAAAAGCCAA CGGTCCAAAG CACCAAACCT 
CTAGCTTCAC CCTCCCCTGT TGAAAAGCAG GATGTCAGAG AGTCCCACCC AGAGTGCTCA 
GAAACTGAAG CCCCTACTGC ACGTCGGGCC GTGCCTACCT CGCGCTTTCG TGATTTCTTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297955.1
CDS	388..5730
Translation	MNGVAFCLVGIPPYPESEAKGRRDGLSSSSENPRPVVRSGTDGLGVSWQGPRTLVLQKNSQGFGFTLRHFIVYPPESALHTTLKDEENGNGKGLQRSRLEPMDTIFVKNVREKGPAHLAGLCTGDRLVKVNGESVLGKTYSQVIALIQNSESVLELSIMPKDEDVLQLAYSHDAYLRGNNPYTGKAQNLPPPPPVLYPPRTKSQSPAGFSPCPPPASSHMGQNQLDNWSRWPGSSAGPSPPLDNRTVASSNVVWAAEGRNCEPGGVGHSSPAHRTEEIQYGMTDRGQQPTGQMRGRSYSSSSSSGGMMSPLHHGHLANHNMGTAMGHTGGRKGSPPWASPSQPTSTPSSSRNERTQQALSNWYYSQVPGRERERERERERSSSRSMHPRHRSFSQDRLGELGKARRSQQAGFPHSASQDTLLLLQQSAAAYADPYWNHGDWRGEHMYGQHPSVVAYGRKSSENLLASRYGHSGRSLENLDRAVGILSPRVERSAWVQQPPNRTEVYARHAGHFGQHIESPHQMHHTPQHHQNLTHATHQHSSQIPQPQQAAPMARRLPSCQSLDEEPIGYRSYSPSFNRKTGRILQQAQSFRDPSYTGPHLWTPTSKTSPPEGHPPSATASPAPPTTSSSTTISTPSEGQDRTYRPTNHDRGAGEATSEAQTQEVVLRQKPPTGRRGANALRHPHYAIALDSSDPLLFTTDPTEPTAKAESAISHRRTDVNLPPLSIEDDSLASIPFIDEPTSPSADLRARHVPASSVVSSGGINSAPAVITSPASPTFTFPLNRLFSHNCSDPKSNRRSSYLLAITTERSKSCDEGLNAYREEGRVFSRLPKRVKSFFTEGSLDSLGAAEEARSKRHSTSELGNISYSDVKREGWLHFKQIHTEKGKKVGSAIWPWRRVFSVLRTHSLFLYKDKREAVLKGAQLGGGAEDEQPISIRGCLVDIAYSETKRKHALRLTTQDFCEYLLQAEDREDMLEWIRVIRDNSKTDNEELGFSRQALINKKLNDYRKQSPTGNKPDSSPRVHRMTFLLSKPDNSAPPRSPKHEAKDESSPPKSPWGINIMKKSKKAGPKAFGVRLEDCQPAVNNKFIPQIVEICCGLVEDMGLEYTGIYRVPGNNAVVSSLQEQLNKGVDINITEEKWQDLNVVSSLLKSFFRKLPEPLFTDDKYNDFIDANRMENASDRLRTMKKLIRDLPDHYFHTLKFLVGHLKTVADHSEKNKMEPRNLALVFGPTLVRTSEDNMTDMVTHMPDRYKIVETLIQHYTWFFCEEQDKDEKTPVDTEDVQPAPNIDHLLSNIGRTGMLGEASDSTNSDSAKSKGSWGSKRDFTAKDILTLSIMSAVTRKRRKRHNGRLLGSSTDDDSEHEPIKSCISEKDDLQEMGQVASFCAPRAEGEEDDDEEEEDDEEEEEGAERQKAQEQKETVVPSISSTEEVTSTVLLSEEEEQKSEVKGGRSWRLDDARSIVSGYSTLSTLGRSLASEGRGEDADDEHSELVSETDNESGFASRSLTQERPEKPNRSNQNSHASPEPTAPQSFLYAHCKSPTPSGSATAPSPPSQHITTNPEDSAARSSTPSTSSYSSSASQRLHNRSFNSHRLIQCDTLARRRTKADKAKAPSVDLSELSSSSAAEEDRQSGANQIPDSSPSPSSKAKGFSIRNECTPISMVGPGSKGCVLSPGQGSLADQVRARLMGSADDLCSVGLRKQLSPETRRKRRAWRRHTVVVSPTDCSDKKPTVQSTKPLASPSPVEKQDVRESHPECSETEAPTARRAVPTSRFRDFL

Target ORF information:

RefSeq Version	XM_009299680.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Rho GTPase activating protein 23a (arhgap23a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299680.2

ORF Insert Sequence:

ATGAATGGAG TTGCATTTTG CCTTGTTGGA ATACCTCCGT ATCCAGAAAG CGAAGCAAAA 
GGGCGGAGAG ATGGGCTCTC TTCATCCAGT GAGAACCCTC GGCCGGTGGT CCGGAGCGGG 
ACAGATGGGC TGGGCGTGTC CTGGCAGGGT CCACGCACGC TGGTGCTCCA AAAGAACTCT 
CAGGGCTTCG GCTTTACTCT GCGTCACTTC ATCGTTTACC CGCCAGAGTC CGCTCTGCAC 
ACCACTCTCA AGGATGAAGA GAACGGGAAT GGAAAGGGGC TTCAACGAAG TCGTCTGGAG 
CCCATGGACA CAATTTTTGT GAAGAATGTG AGGGAAAAAG GCCCCGCTCA CCTGGCTGGC 
CTGTGTACAG GAGACAGGCT GGTTAAAGTG AATGGGGAGA GCGTGCTGGG TAAGACGTAC 
TCGCAGGTCA TTGCACTGAT TCAGAACAGT GAGAGTGTAC TGGAGCTCTC CATTATGCCA 
AAGGATGAGG ACGTGCTTCA GCTGGCATAT TCCCATGATG CCTACCTAAG GGGGAACAAT 
CCATATACAG GAAAGGCCCA AAATCTTCCT CCACCACCCC CTGTTCTCTA CCCTCCTCGT 
ACAAAATCAC AGTCTCCTGC AGGGTTCAGC CCCTGCCCTC CTCCAGCTTC TTCACACATG 
GGGCAAAACC AGCTGGACAA CTGGAGCCGC TGGCCCGGCT CTTCTGCTGG TCCCTCTCCA 
CCCCTTGACA ACCGGACTGT GGCCTCCAGC AATGTTGTCT GGGCAGCCGA GGGCAGGAAT 
TGTGAGCCGG GTGGTGTTGG TCACAGTAGC CCTGCACACC GAACAGAGGA GATCCAGTAT 
GGAATGACTG ACAGAGGACA GCAGCCAACT GGACAAATGA GAGGACGCTC ATACTCATCT 
TCTTCCTCCT CAGGAGGAAT GATGAGCCCT TTGCATCATG GTCATCTTGC CAATCACAAC 
ATGGGTACTG CCATGGGGCA CACTGGAGGT CGTAAGGGAA GCCCACCTTG GGCCAGCCCT 
TCTCAGCCAA CATCAACACC TTCATCCAGC CGCAACGAAC GAACCCAACA AGCCCTTTCC 
AACTGGTACT ACAGCCAGGT CCCAGGCCGT GAGCGAGAAC GAGAACGAGA GCGGGAGCGA 
TCTAGTTCTC GCTCAATGCA CCCACGCCAC AGGAGTTTCT CACAAGACCG TCTGGGAGAG 
CTTGGGAAGG CCAGGCGTTC TCAACAAGCA GGTTTTCCCC ATAGTGCATC GCAAGACACG 
TTGCTGCTGC TTCAGCAGAG TGCTGCAGCA TATGCTGATC CTTACTGGAA CCATGGAGAC 
TGGCGGGGTG AGCATATGTA TGGTCAGCAC CCATCTGTGG TGGCCTATGG GCGAAAGTCC 
TCCGAAAATC TTCTAGCAAG CCGGTACGGA CACTCGGGAC GCTCACTGGA AAATCTCGAC 
AGAGCTGTGG GGATACTCTC ACCAAGGGTT GAAAGATCTG CTTGGGTTCA ACAACCTCCA 
AATAGGACTG AAGTCTATGC AAGACATGCT GGGCATTTCG GCCAACATAT AGAGTCACCC 
CACCAAATGC ACCACACACC CCAACACCAC CAGAATCTCA CACATGCAAC ACACCAACAC 
TCATCTCAAA TCCCACAACC TCAGCAGGCA GCTCCAATGG CAAGACGTTT GCCTTCTTGC 
CAGAGTCTAG ATGAGGAGCC GATTGGCTAT CGTAGCTACA GTCCCTCATT CAATAGGAAG 
ACTGGCCGTA TCTTGCAGCA GGCCCAGTCA TTTAGAGATC CCTCTTACAC AGGTCCACAC 
CTTTGGACTC CAACATCCAA AACCAGTCCC CCAGAGGGGC ATCCACCCTC AGCCACTGCT 
TCCCCAGCCC CACCAACCAC TTCCTCTTCA ACTACGATTT CCACACCATC TGAAGGACAA 
GACAGAACTT ACCGACCCAC TAATCATGAC AGAGGGGCAG GGGAAGCGAC AAGTGAAGCC 
CAGACACAGG AAGTGGTTCT GAGGCAGAAA CCACCTACCG GCCGACGAGG TGCAAACGCC 
CTTCGACACC CACATTATGC AATAGCACTT GACTCCAGTG ACCCTCTACT CTTTACCACT 
GACCCTACGG AGCCCACTGC CAAGGCAGAA AGTGCTATTT CTCATCGCCG CACAGATGTT 
AACCTTCCTC CACTATCTAT TGAGGATGAC TCCCTTGCAT CAATTCCATT CATCGATGAG 
CCGACCAGTC CAAGTGCAGA TCTGCGGGCA CGTCATGTCC CCGCCTCCTC CGTCGTGTCC 
AGTGGCGGTA TAAACTCCGC CCCTGCTGTG ATCACAAGCC CCGCCTCCCC AACCTTCACC 
TTTCCCCTCA ACCGCCTTTT CTCCCACAAC TGCAGTGATC CTAAATCTAA TCGTCGCTCC 
TCGTACCTGT TGGCCATCAC CACTGAACGC TCCAAGTCAT GTGATGAGGG GCTTAACGCA 
TATAGAGAAG AGGGCCGGGT CTTCTCGAGG CTTCCAAAAA GAGTCAAGAG CTTCTTTACT 
GAAGGGTCTC TGGACAGTCT GGGTGCTGCT GAAGAGGCTC GCTCTAAACG ACACTCTACC 
TCTGAGCTGG GCAACATCAG CTACAGTGAC GTGAAGAGAG AAGGCTGGCT CCACTTCAAA 
CAGATCCACA CGGAGAAGGG CAAGAAGGTT GGCAGTGCAA TCTGGCCATG GAGACGGGTG 
TTCTCTGTGC TTAGGACCCA TTCTCTGTTT CTTTATAAAG ACAAGCGAGA GGCAGTGCTT 
AAAGGGGCGC AATTAGGGGG CGGGGCTGAA GACGAGCAGC CAATCAGCAT CCGGGGCTGC 
CTTGTGGATA TAGCATACAG TGAAACGAAG CGCAAGCACG CGCTGAGATT GACCACGCAG 
GACTTCTGCG AGTACCTGCT GCAGGCGGAG GACAGAGAGG ACATGCTGGA GTGGATCCGA 
GTCATCAGGG ACAACAGCAA GACCGACAAC GAGGAATTGG GTTTCTCACG ACAAGCTCTC 
ATCAATAAAA AGCTTAACGA CTACAGGAAG CAAAGTCCAA CGGGTAACAA ACCAGACTCC 
TCACCCAGAG TGCATCGAAT GACCTTCCTC CTCAGCAAGC CAGACAACAG CGCACCTCCA 
CGCTCCCCAA AACACGAGGC CAAAGATGAG AGCAGCCCCC CAAAATCTCC CTGGGGTATA 
AACATAATGA AGAAGAGCAA GAAGGCCGGT CCTAAAGCTT TTGGCGTCCG TCTAGAGGAC 
TGCCAGCCTG CAGTAAACAA CAAGTTCATT CCTCAGATTG TGGAGATCTG CTGTGGGCTG 
GTGGAGGACA TGGGTCTGGA GTATACAGGG ATCTACAGAG TGCCAGGAAA CAATGCTGTA 
GTCTCCAGTC TGCAGGAGCA GCTCAACAAA GGAGTAGACA TTAACATCAC TGAGGAGAAA 
TGGCAGGACC TGAATGTGGT GAGCAGCCTG CTGAAGTCCT TCTTTCGTAA ACTCCCTGAA 
CCCCTCTTCA CTGATGATAA ATATAATGAC TTTATCGACG CCAATCGGAT GGAGAACGCC 
AGCGATAGGC TGAGGACAAT GAAGAAATTG ATTCGGGACC TACCAGATCA TTACTTTCAC 
ACTCTGAAAT TTTTAGTTGG ACACCTAAAG ACGGTGGCTG ACCACTCTGA GAAAAACAAA 
ATGGAGCCGC GTAATTTGGC TCTGGTGTTT GGCCCCACTC TTGTTCGAAC TTCAGAGGAC 
AACATGACAG ACATGGTCAC CCACATGCCA GACCGCTACA AGATTGTGGA GACACTCATT 
CAGCATTACA CCTGGTTTTT CTGCGAAGAA CAGGATAAGG ATGAAAAGAC CCCAGTGGAC 
ACAGAGGATG TCCAGCCTGC CCCAAACATT GATCATCTGC TCTCCAACAT TGGCCGCACA 
GGCATGCTGG GTGAGGCTTC AGACTCAACC AACAGTGATT CTGCAAAATC TAAGGGGTCA 
TGGGGATCAA AACGTGACTT TACTGCCAAG GATATCCTTA CATTATCAAT TATGTCGGCT 
GTGACCCGCA AACGGCGGAA GCGGCACAAC GGCCGTCTCC TCGGCAGCAG CACAGACGAC 
GATTCTGAGC ATGAACCCAT TAAATCCTGC ATCTCCGAGA AAGACGATCT GCAAGAGATG 
GGGCAGGTTG CATCCTTCTG TGCCCCACGA GCAGAGGGAG AGGAGGATGA CGATGAAGAG 
GAGGAAGATG ATGAAGAGGA GGAGGAGGGG GCTGAGAGAC AGAAGGCACA AGAGCAGAAG 
GAAACAGTAG TCCCCAGCAT TTCCTCAACA GAGGAAGTGA CATCAACAGT GCTGCTGAGC 
GAAGAGGAAG AGCAGAAGTC TGAAGTGAAA GGAGGACGCA GCTGGAGATT AGATGATGCT 
CGGTCTATTG TGTCAGGCTA CTCTACTCTG TCCACTTTGG GGCGGAGCTT AGCTTCAGAG 
GGGCGGGGCG AGGATGCAGA TGATGAGCAT AGTGAACTAG TGAGTGAAAC TGATAATGAA 
AGCGGGTTTG CCTCACGCTC CCTCACCCAA GAAAGGCCAG AAAAACCAAA CCGTTCCAAC 
CAGAACTCTC ATGCGTCTCC TGAACCAACC GCACCACAGA GTTTCCTCTA TGCGCACTGC 
AAATCACCTA CACCCTCTGG CTCCGCCACT GCTCCATCTC CCCCTTCACA ACACATCACA 
ACCAACCCAG AGGACAGCGC AGCACGCTCG TCTACTCCCT CAACCTCCTC TTACTCCTCA 
TCCGCCTCAC AACGGCTCCA CAACCGCTCT TTCAACTCCC ACCGCCTCAT ACAGTGCGAC 
ACCCTTGCAC GCCGTCGGAC AAAGGCGGAC AAAGCCAAAG CTCCCTCAGT GGACCTTTCC 
GAACTCTCGA GCTCCTCCGC CGCAGAGGAG GACCGCCAGT CAGGTGCCAA TCAAATCCCT 
GACTCCTCTC CGTCTCCTTC TTCAAAAGCC AAAGGGTTTA GCATAAGAAA CGAGTGTACG 
CCAATATCCA TGGTTGGTCC CGGAAGTAAG GGGTGTGTGT TGTCCCCCGG GCAAGGATCC 
CTGGCGGATC AGGTACGTGC TCGTCTAATG GGCTCGGCGG ATGACCTGTG CAGCGTTGGC 
CTGAGAAAGC AGCTCTCGCC GGAGACGCGC CGGAAGAGAC GCGCTTGGAG GAGACACACA 
GTGGTCGTGT CCCCTACGGA TTGTTCAGAC AAAAAGCCAA CGGTCCAAAG CACCAAACCT 
CTAGCTTCAC CCTCCCCTGT TGAAAAGCAG GATGTCAGAG AGTCCCACCC AGAGTGCTCA 
GAAACTGAAG CCCCTACTGC ACGTCGGGCC GTGCCTACCT CGCGCTTTCG TGATTTCTTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap23a ( XM_009299681.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009297956.1 Danio rerio Rho GTPase activating protein 23a (arhgap23a), transcript variant X4, mRNA.

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CloneID	ODa48128
Clone ID Related Accession (Same CDS sequence)	XM_009299681.2 , XM_021474705.1
Accession Version	XM_009299681.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	rho GTPase-activating protein 23 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299681.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG CAAAAGGGCG GAGAGATGGG CTCTCTTCAT CCAGTGAGAA CCCTCGGCCG 
GTGGTCCGGA GCGGGACAGA TGGGCTGGGC GTGTCCTGGC AGGGTCCACG CACGCTGGTG 
CTCCAAAAGA ACTCTCAGGG CTTCGGCTTT ACTCTGCGTC ACTTCATCGT TTACCCGCCA 
GAGTCCGCTC TGCACACCAC TCTCAAGGAT GAAGAGAACG GGAATGGAAA GGGGCTTCAA 
CGAAGTCGTC TGGAGCCCAT GGACACAATT TTTGTGAAGA ATGTGAGGGA AAAAGGCCCC 
GCTCACCTGG CTGGCCTGTG TACAGGAGAC AGGCTGGTTA AAGTGAATGG GGAGAGCGTG 
CTGGGTAAGA CGTACTCGCA GGTCATTGCA CTGATTCAGA ACAGTGAGAG TGTACTGGAG 
CTCTCCATTA TGCCAAAGGA TGAGGACGTG CTTCAGCTGG CATATTCCCA TGATGCCTAC 
CTAAGGGGGA ACAATCCATA TACAGGAAAG GCCCAAAATC TTCCTCCACC ACCCCCTGTT 
CTCTACCCTC CTCGTACAAA ATCACAGTCT CCTGCAGGGT TCAGCCCCTG CCCTCCTCCA 
GCTTCTTCAC ACATGGGGCA AAACCAGCTG GACAACTGGA GCCGCTGGCC CGGCTCTTCT 
GCTGGTCCCT CTCCACCCCT TGACAACCGG ACTGTGGCCT CCAGCAATGT TGTCTGGGCA 
GCCGAGGGCA GGAATTGTGA GCCGGGTGGT GTTGGTCACA GTAGCCCTGC ACACCGAACA 
GAGGAGATCC AGTATGGAAT GACTGACAGA GGACAGCAGC CAACTGGACA AATGAGAGGA 
CGCTCATACT CATCTTCTTC CTCCTCAGGA GGAATGATGA GCCCTTTGCA TCATGGTCAT 
CTTGCCAATC ACAACATGGG TACTGCCATG GGGCACACTG GAGGTCGTAA GGGAAGCCCA 
CCTTGGGCCA GCCCTTCTCA GCCAACATCA ACACCTTCAT CCAGCCGCAA CGAACGAACC 
CAACAAGCCC TTTCCAACTG GTACTACAGC CAGGTCCCAG GCCGTGAGCG AGAACGAGAA 
CGAGAGCGGG AGCGATCTAG TTCTCGCTCA ATGCACCCAC GCCACAGGAG TTTCTCACAA 
GACCGTCTGG GAGAGCTTGG GAAGGCCAGG CGTTCTCAAC AAGCAGGTTT TCCCCATAGT 
GCATCGCAAG ACACGTTGCT GCTGCTTCAG CAGAGTGCTG CAGCATATGC TGATCCTTAC 
TGGAACCATG GAGACTGGCG GGGTGAGCAT ATGTATGGTC AGCACCCATC TGTGGTGGCC 
TATGGGCGAA AGTCCTCCGA AAATCTTCTA GCAAGCCGGT ACGGACACTC GGGACGCTCA 
CTGGAAAATC TCGACAGAGC TGTGGGGATA CTCTCACCAA GGGTTGAAAG ATCTGCTTGG 
GTTCAACAAC CTCCAAATAG GACTGAAGTC TATGCAAGAC ATGCTGGGCA TTTCGGCCAA 
CATATAGAGT CACCCCACCA AATGCACCAC ACACCCCAAC ACCACCAGAA TCTCACACAT 
GCAACACACC AACACTCATC TCAAATCCCA CAACCTCAGC AGGCAGCTCC AATGGCAAGA 
CGTTTGCCTT CTTGCCAGAG TCTAGATGAG GAGCCGATTG GCTATCGTAG CTACAGTCCC 
TCATTCAATA GGAAGACTGG CCGTATCTTG CAGCAGGCCC AGTCATTTAG AGATCCCTCT 
TACACAGGTC CACACCTTTG GACTCCAACA TCCAAAACCA GTCCCCCAGA GGGGCATCCA 
CCCTCAGCCA CTGCTTCCCC AGCCCCACCA ACCACTTCCT CTTCAACTAC GATTTCCACA 
CCATCTGAAG GACAAGACAG AACTTACCGA CCCACTAATC ATGACAGAGG GGCAGGGGAA 
GCGACAAGTG AAGCCCAGAC ACAGGAAGTG GTTCTGAGGC AGAAACCACC TACCGGCCGA 
CGAGGTGCAA ACGCCCTTCG ACACCCACAT TATGCAATAG CACTTGACTC CAGTGACCCT 
CTACTCTTTA CCACTGACCC TACGGAGCCC ACTGCCAAGG CAGAAAGTGC TATTTCTCAT 
CGCCGCACAG ATGTTAACCT TCCTCCACTA TCTATTGAGG ATGACTCCCT TGCATCAATT 
CCATTCATCG ATGAGCCGAC CAGTCCAAGT GCAGATCTGC GGGCACGTCA TGTCCCCGCC 
TCCTCCGTCG TGTCCAGTGG CGGTATAAAC TCCGCCCCTG CTGTGATCAC AAGCCCCGCC 
TCCCCAACCT TCACCTTTCC CCTCAACCGC CTTTTCTCCC ACAACTGCAG TGATCCTAAA 
TCTAATCGTC GCTCCTCGTA CCTGTTGGCC ATCACCACTG AACGCTCCAA GTCATGTGAT 
GAGGGGCTTA ACGCATATAG AGAAGAGGGC CGGGTCTTCT CGAGGCTTCC AAAAAGAGTC 
AAGAGCTTCT TTACTGAAGG GTCTCTGGAC AGTCTGGGTG CTGCTGAAGA GGCTCGCTCT 
AAACGACACT CTACCTCTGA GCTGGGCAAC ATCAGCTACA GTGACGTGAA GAGAGAAGGC 
TGGCTCCACT TCAAACAGAT CCACACGGAG AAGGGCAAGA AGGTTGGCAG TGCAATCTGG 
CCATGGAGAC GGGTGTTCTC TGTGCTTAGG ACCCATTCTC TGTTTCTTTA TAAAGACAAG 
CGAGAGGCAG TGCTTAAAGG GGCGCAATTA GGGGGCGGGG CTGAAGACGA GCAGCCAATC 
AGCATCCGGG GCTGCCTTGT GGATATAGCA TACAGTGAAA CGAAGCGCAA GCACGCGCTG 
AGATTGACCA CGCAGGACTT CTGCGAGTAC CTGCTGCAGG CGGAGGACAG AGAGGACATG 
CTGGAGTGGA TCCGAGTCAT CAGGGACAAC AGCAAGACCG ACAACGAGGA ATTGGGTTTC 
TCACGACAAG CTCTCATCAA TAAAAAGCTT AACGACTACA GGAAGCAAAG TCCAACGGGT 
AACAAACCAG ACTCCTCACC CAGAGTGCAT CGAATGACCT TCCTCCTCAG CAAGCCAGAC 
AACAGCGCAC CTCCACGCTC CCCAAAACAC GAGGCCAAAG ATGAGAGCAG CCCCCCAAAA 
TCTCCCTGGG GTATAAACAT AATGAAGAAG AGCAAGAAGG CCGGTCCTAA AGCTTTTGGC 
GTCCGTCTAG AGGACTGCCA GCCTGCAGTA AACAACAAGT TCATTCCTCA GATTGTGGAG 
ATCTGCTGTG GGCTGGTGGA GGACATGGGT CTGGAGTATA CAGGGATCTA CAGAGTGCCA 
GGAAACAATG CTGTAGTCTC CAGTCTGCAG GAGCAGCTCA ACAAAGGAGT AGACATTAAC 
ATCACTGAGG AGAAATGGCA GGACCTGAAT GTGGTGAGCA GCCTGCTGAA GTCCTTCTTT 
CGTAAACTCC CTGAACCCCT CTTCACTGAT GATAAATATA ATGACTTTAT CGACGCCAAT 
CGGATGGAGA ACGCCAGCGA TAGGCTGAGG ACAATGAAGA AATTGATTCG GGACCTACCA 
GATCATTACT TTCACACTCT GAAATTTTTA GTTGGACACC TAAAGACGGT GGCTGACCAC 
TCTGAGAAAA ACAAAATGGA GCCGCGTAAT TTGGCTCTGG TGTTTGGCCC CACTCTTGTT 
CGAACTTCAG AGGACAACAT GACAGACATG GTCACCCACA TGCCAGACCG CTACAAGATT 
GTGGAGACAC TCATTCAGCA TTACACCTGG TTTTTCTGCG AAGAACAGGA TAAGGATGAA 
AAGACCCCAG TGGACACAGA GGATGTCCAG CCTGCCCCAA ACATTGATCA TCTGCTCTCC 
AACATTGGCC GCACAGGCAT GCTGGGTGAG GCTTCAGACT CAACCAACAG TGATTCTGCA 
AAATCTAAGG GGTCATGGGG ATCAAAACGT GACTTTACTG CCAAGGATAT CCTTACATTA 
TCAATTATGT CGGCTGTGAC CCGCAAACGG CGGAAGCGGC ACAACGGCCG TCTCCTCGGC 
AGCAGCACAG ACGACGATTC TGAGCATGAA CCCATTAAAT CCTGCATCTC CGAGAAAGAC 
GATCTGCAAG AGATGGGGCA GGTTGCATCC TTCTGTGCCC CACGAGCAGA GGGAGAGGAG 
GATGACGATG AAGAGGAGGA AGATGATGAA GAGGAGGAGG AGGGGGCTGA GAGACAGAAG 
GCACAAGAGC AGAAGGAAAC AGTAGTCCCC AGCATTTCCT CAACAGAGGA AGTGACATCA 
ACAGTGCTGC TGAGCGAAGA GGAAGAGCAG AAGTCTGAAG TGAAAGGAGG ACGCAGCTGG 
AGATTAGATG ATGCTCGGTC TATTGTGTCA GGCTACTCTA CTCTGTCCAC TTTGGGGCGG 
AGCTTAGCTT CAGAGGGGCG GGGCGAGGAT GCAGATGATG AGCATAGTGA ACTAGTGAGT 
GAAACTGATA ATGAAAGCGG GTTTGCCTCA CGCTCCCTCA CCCAAGAAAG GCCAGAAAAA 
CCAAACCGTT CCAACCAGAA CTCTCATGCG TCTCCTGAAC CAACCGCACC ACAGAGTTTC 
CTCTATGCGC ACTGCAAATC ACCTACACCC TCTGGCTCCG CCACTGCTCC ATCTCCCCCT 
TCACAACACA TCACAACCAA CCCAGAGGAC AGCGCAGCAC GCTCGTCTAC TCCCTCAACC 
TCCTCTTACT CCTCATCCGC CTCACAACGG CTCCACAACC GCTCTTTCAA CTCCCACCGC 
CTCATACAGT GCGACACCCT TGCACGCCGT CGGACAAAGG CGGACAAAGC CAAAGCTCCC 
TCAGTGGACC TTTCCGAACT CTCGAGCTCC TCCGCCGCAG AGGAGGACCG CCAGTCAGGT 
GCCAATCAAA TCCCTGACTC CTCTCCGTCT CCTTCTTCAA AAGCCAAAGG GTTTAGCATA 
AGAAACGAGT GTACGCCAAT ATCCATGGTT GGTCCCGGAA GTAAGGGGTG TGTGTTGTCC 
CCCGGGCAAG GATCCCTGGC GGATCAGGTA CGTGCTCGTC TAATGGGCTC GGCGGATGAC 
CTGTGCAGCG TTGGCCTGAG AAAGCAGCTC TCGCCGGAGA CGCGCCGGAA GAGACGCGCT 
TGGAGGAGAC ACACAGTGGT CGTGTCCCCT ACGGATTGTT CAGACAAAAA GCCAACGGTC 
CAAAGCACCA AACCTCTAGC TTCACCCTCC CCTGTTGAAA AGCAGGATGT CAGAGAGTCC 
CACCCAGAGT GCTCAGAAAC TGAAGCCCCT ACTGCACGTC GGGCCGTGCC TACCTCGCGC 
TTTCGTGATT TCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297956.1
CDS	854..6151
Translation	MEQAKGRRDGLSSSSENPRPVVRSGTDGLGVSWQGPRTLVLQKNSQGFGFTLRHFIVYPPESALHTTLKDEENGNGKGLQRSRLEPMDTIFVKNVREKGPAHLAGLCTGDRLVKVNGESVLGKTYSQVIALIQNSESVLELSIMPKDEDVLQLAYSHDAYLRGNNPYTGKAQNLPPPPPVLYPPRTKSQSPAGFSPCPPPASSHMGQNQLDNWSRWPGSSAGPSPPLDNRTVASSNVVWAAEGRNCEPGGVGHSSPAHRTEEIQYGMTDRGQQPTGQMRGRSYSSSSSSGGMMSPLHHGHLANHNMGTAMGHTGGRKGSPPWASPSQPTSTPSSSRNERTQQALSNWYYSQVPGRERERERERERSSSRSMHPRHRSFSQDRLGELGKARRSQQAGFPHSASQDTLLLLQQSAAAYADPYWNHGDWRGEHMYGQHPSVVAYGRKSSENLLASRYGHSGRSLENLDRAVGILSPRVERSAWVQQPPNRTEVYARHAGHFGQHIESPHQMHHTPQHHQNLTHATHQHSSQIPQPQQAAPMARRLPSCQSLDEEPIGYRSYSPSFNRKTGRILQQAQSFRDPSYTGPHLWTPTSKTSPPEGHPPSATASPAPPTTSSSTTISTPSEGQDRTYRPTNHDRGAGEATSEAQTQEVVLRQKPPTGRRGANALRHPHYAIALDSSDPLLFTTDPTEPTAKAESAISHRRTDVNLPPLSIEDDSLASIPFIDEPTSPSADLRARHVPASSVVSSGGINSAPAVITSPASPTFTFPLNRLFSHNCSDPKSNRRSSYLLAITTERSKSCDEGLNAYREEGRVFSRLPKRVKSFFTEGSLDSLGAAEEARSKRHSTSELGNISYSDVKREGWLHFKQIHTEKGKKVGSAIWPWRRVFSVLRTHSLFLYKDKREAVLKGAQLGGGAEDEQPISIRGCLVDIAYSETKRKHALRLTTQDFCEYLLQAEDREDMLEWIRVIRDNSKTDNEELGFSRQALINKKLNDYRKQSPTGNKPDSSPRVHRMTFLLSKPDNSAPPRSPKHEAKDESSPPKSPWGINIMKKSKKAGPKAFGVRLEDCQPAVNNKFIPQIVEICCGLVEDMGLEYTGIYRVPGNNAVVSSLQEQLNKGVDINITEEKWQDLNVVSSLLKSFFRKLPEPLFTDDKYNDFIDANRMENASDRLRTMKKLIRDLPDHYFHTLKFLVGHLKTVADHSEKNKMEPRNLALVFGPTLVRTSEDNMTDMVTHMPDRYKIVETLIQHYTWFFCEEQDKDEKTPVDTEDVQPAPNIDHLLSNIGRTGMLGEASDSTNSDSAKSKGSWGSKRDFTAKDILTLSIMSAVTRKRRKRHNGRLLGSSTDDDSEHEPIKSCISEKDDLQEMGQVASFCAPRAEGEEDDDEEEEDDEEEEEGAERQKAQEQKETVVPSISSTEEVTSTVLLSEEEEQKSEVKGGRSWRLDDARSIVSGYSTLSTLGRSLASEGRGEDADDEHSELVSETDNESGFASRSLTQERPEKPNRSNQNSHASPEPTAPQSFLYAHCKSPTPSGSATAPSPPSQHITTNPEDSAARSSTPSTSSYSSSASQRLHNRSFNSHRLIQCDTLARRRTKADKAKAPSVDLSELSSSSAAEEDRQSGANQIPDSSPSPSSKAKGFSIRNECTPISMVGPGSKGCVLSPGQGSLADQVRARLMGSADDLCSVGLRKQLSPETRRKRRAWRRHTVVVSPTDCSDKKPTVQSTKPLASPSPVEKQDVRESHPECSETEAPTARRAVPTSRFRDFL

Target ORF information:

RefSeq Version	XM_009299681.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Rho GTPase activating protein 23a (arhgap23a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299681.2

ORF Insert Sequence:

ATGGAGCAGG CAAAAGGGCG GAGAGATGGG CTCTCTTCAT CCAGTGAGAA CCCTCGGCCG 
GTGGTCCGGA GCGGGACAGA TGGGCTGGGC GTGTCCTGGC AGGGTCCACG CACGCTGGTG 
CTCCAAAAGA ACTCTCAGGG CTTCGGCTTT ACTCTGCGTC ACTTCATCGT TTACCCGCCA 
GAGTCCGCTC TGCACACCAC TCTCAAGGAT GAAGAGAACG GGAATGGAAA GGGGCTTCAA 
CGAAGTCGTC TGGAGCCCAT GGACACAATT TTTGTGAAGA ATGTGAGGGA AAAAGGCCCC 
GCTCACCTGG CTGGCCTGTG TACAGGAGAC AGGCTGGTTA AAGTGAATGG GGAGAGCGTG 
CTGGGTAAGA CGTACTCGCA GGTCATTGCA CTGATTCAGA ACAGTGAGAG TGTACTGGAG 
CTCTCCATTA TGCCAAAGGA TGAGGACGTG CTTCAGCTGG CATATTCCCA TGATGCCTAC 
CTAAGGGGGA ACAATCCATA TACAGGAAAG GCCCAAAATC TTCCTCCACC ACCCCCTGTT 
CTCTACCCTC CTCGTACAAA ATCACAGTCT CCTGCAGGGT TCAGCCCCTG CCCTCCTCCA 
GCTTCTTCAC ACATGGGGCA AAACCAGCTG GACAACTGGA GCCGCTGGCC CGGCTCTTCT 
GCTGGTCCCT CTCCACCCCT TGACAACCGG ACTGTGGCCT CCAGCAATGT TGTCTGGGCA 
GCCGAGGGCA GGAATTGTGA GCCGGGTGGT GTTGGTCACA GTAGCCCTGC ACACCGAACA 
GAGGAGATCC AGTATGGAAT GACTGACAGA GGACAGCAGC CAACTGGACA AATGAGAGGA 
CGCTCATACT CATCTTCTTC CTCCTCAGGA GGAATGATGA GCCCTTTGCA TCATGGTCAT 
CTTGCCAATC ACAACATGGG TACTGCCATG GGGCACACTG GAGGTCGTAA GGGAAGCCCA 
CCTTGGGCCA GCCCTTCTCA GCCAACATCA ACACCTTCAT CCAGCCGCAA CGAACGAACC 
CAACAAGCCC TTTCCAACTG GTACTACAGC CAGGTCCCAG GCCGTGAGCG AGAACGAGAA 
CGAGAGCGGG AGCGATCTAG TTCTCGCTCA ATGCACCCAC GCCACAGGAG TTTCTCACAA 
GACCGTCTGG GAGAGCTTGG GAAGGCCAGG CGTTCTCAAC AAGCAGGTTT TCCCCATAGT 
GCATCGCAAG ACACGTTGCT GCTGCTTCAG CAGAGTGCTG CAGCATATGC TGATCCTTAC 
TGGAACCATG GAGACTGGCG GGGTGAGCAT ATGTATGGTC AGCACCCATC TGTGGTGGCC 
TATGGGCGAA AGTCCTCCGA AAATCTTCTA GCAAGCCGGT ACGGACACTC GGGACGCTCA 
CTGGAAAATC TCGACAGAGC TGTGGGGATA CTCTCACCAA GGGTTGAAAG ATCTGCTTGG 
GTTCAACAAC CTCCAAATAG GACTGAAGTC TATGCAAGAC ATGCTGGGCA TTTCGGCCAA 
CATATAGAGT CACCCCACCA AATGCACCAC ACACCCCAAC ACCACCAGAA TCTCACACAT 
GCAACACACC AACACTCATC TCAAATCCCA CAACCTCAGC AGGCAGCTCC AATGGCAAGA 
CGTTTGCCTT CTTGCCAGAG TCTAGATGAG GAGCCGATTG GCTATCGTAG CTACAGTCCC 
TCATTCAATA GGAAGACTGG CCGTATCTTG CAGCAGGCCC AGTCATTTAG AGATCCCTCT 
TACACAGGTC CACACCTTTG GACTCCAACA TCCAAAACCA GTCCCCCAGA GGGGCATCCA 
CCCTCAGCCA CTGCTTCCCC AGCCCCACCA ACCACTTCCT CTTCAACTAC GATTTCCACA 
CCATCTGAAG GACAAGACAG AACTTACCGA CCCACTAATC ATGACAGAGG GGCAGGGGAA 
GCGACAAGTG AAGCCCAGAC ACAGGAAGTG GTTCTGAGGC AGAAACCACC TACCGGCCGA 
CGAGGTGCAA ACGCCCTTCG ACACCCACAT TATGCAATAG CACTTGACTC CAGTGACCCT 
CTACTCTTTA CCACTGACCC TACGGAGCCC ACTGCCAAGG CAGAAAGTGC TATTTCTCAT 
CGCCGCACAG ATGTTAACCT TCCTCCACTA TCTATTGAGG ATGACTCCCT TGCATCAATT 
CCATTCATCG ATGAGCCGAC CAGTCCAAGT GCAGATCTGC GGGCACGTCA TGTCCCCGCC 
TCCTCCGTCG TGTCCAGTGG CGGTATAAAC TCCGCCCCTG CTGTGATCAC AAGCCCCGCC 
TCCCCAACCT TCACCTTTCC CCTCAACCGC CTTTTCTCCC ACAACTGCAG TGATCCTAAA 
TCTAATCGTC GCTCCTCGTA CCTGTTGGCC ATCACCACTG AACGCTCCAA GTCATGTGAT 
GAGGGGCTTA ACGCATATAG AGAAGAGGGC CGGGTCTTCT CGAGGCTTCC AAAAAGAGTC 
AAGAGCTTCT TTACTGAAGG GTCTCTGGAC AGTCTGGGTG CTGCTGAAGA GGCTCGCTCT 
AAACGACACT CTACCTCTGA GCTGGGCAAC ATCAGCTACA GTGACGTGAA GAGAGAAGGC 
TGGCTCCACT TCAAACAGAT CCACACGGAG AAGGGCAAGA AGGTTGGCAG TGCAATCTGG 
CCATGGAGAC GGGTGTTCTC TGTGCTTAGG ACCCATTCTC TGTTTCTTTA TAAAGACAAG 
CGAGAGGCAG TGCTTAAAGG GGCGCAATTA GGGGGCGGGG CTGAAGACGA GCAGCCAATC 
AGCATCCGGG GCTGCCTTGT GGATATAGCA TACAGTGAAA CGAAGCGCAA GCACGCGCTG 
AGATTGACCA CGCAGGACTT CTGCGAGTAC CTGCTGCAGG CGGAGGACAG AGAGGACATG 
CTGGAGTGGA TCCGAGTCAT CAGGGACAAC AGCAAGACCG ACAACGAGGA ATTGGGTTTC 
TCACGACAAG CTCTCATCAA TAAAAAGCTT AACGACTACA GGAAGCAAAG TCCAACGGGT 
AACAAACCAG ACTCCTCACC CAGAGTGCAT CGAATGACCT TCCTCCTCAG CAAGCCAGAC 
AACAGCGCAC CTCCACGCTC CCCAAAACAC GAGGCCAAAG ATGAGAGCAG CCCCCCAAAA 
TCTCCCTGGG GTATAAACAT AATGAAGAAG AGCAAGAAGG CCGGTCCTAA AGCTTTTGGC 
GTCCGTCTAG AGGACTGCCA GCCTGCAGTA AACAACAAGT TCATTCCTCA GATTGTGGAG 
ATCTGCTGTG GGCTGGTGGA GGACATGGGT CTGGAGTATA CAGGGATCTA CAGAGTGCCA 
GGAAACAATG CTGTAGTCTC CAGTCTGCAG GAGCAGCTCA ACAAAGGAGT AGACATTAAC 
ATCACTGAGG AGAAATGGCA GGACCTGAAT GTGGTGAGCA GCCTGCTGAA GTCCTTCTTT 
CGTAAACTCC CTGAACCCCT CTTCACTGAT GATAAATATA ATGACTTTAT CGACGCCAAT 
CGGATGGAGA ACGCCAGCGA TAGGCTGAGG ACAATGAAGA AATTGATTCG GGACCTACCA 
GATCATTACT TTCACACTCT GAAATTTTTA GTTGGACACC TAAAGACGGT GGCTGACCAC 
TCTGAGAAAA ACAAAATGGA GCCGCGTAAT TTGGCTCTGG TGTTTGGCCC CACTCTTGTT 
CGAACTTCAG AGGACAACAT GACAGACATG GTCACCCACA TGCCAGACCG CTACAAGATT 
GTGGAGACAC TCATTCAGCA TTACACCTGG TTTTTCTGCG AAGAACAGGA TAAGGATGAA 
AAGACCCCAG TGGACACAGA GGATGTCCAG CCTGCCCCAA ACATTGATCA TCTGCTCTCC 
AACATTGGCC GCACAGGCAT GCTGGGTGAG GCTTCAGACT CAACCAACAG TGATTCTGCA 
AAATCTAAGG GGTCATGGGG ATCAAAACGT GACTTTACTG CCAAGGATAT CCTTACATTA 
TCAATTATGT CGGCTGTGAC CCGCAAACGG CGGAAGCGGC ACAACGGCCG TCTCCTCGGC 
AGCAGCACAG ACGACGATTC TGAGCATGAA CCCATTAAAT CCTGCATCTC CGAGAAAGAC 
GATCTGCAAG AGATGGGGCA GGTTGCATCC TTCTGTGCCC CACGAGCAGA GGGAGAGGAG 
GATGACGATG AAGAGGAGGA AGATGATGAA GAGGAGGAGG AGGGGGCTGA GAGACAGAAG 
GCACAAGAGC AGAAGGAAAC AGTAGTCCCC AGCATTTCCT CAACAGAGGA AGTGACATCA 
ACAGTGCTGC TGAGCGAAGA GGAAGAGCAG AAGTCTGAAG TGAAAGGAGG ACGCAGCTGG 
AGATTAGATG ATGCTCGGTC TATTGTGTCA GGCTACTCTA CTCTGTCCAC TTTGGGGCGG 
AGCTTAGCTT CAGAGGGGCG GGGCGAGGAT GCAGATGATG AGCATAGTGA ACTAGTGAGT 
GAAACTGATA ATGAAAGCGG GTTTGCCTCA CGCTCCCTCA CCCAAGAAAG GCCAGAAAAA 
CCAAACCGTT CCAACCAGAA CTCTCATGCG TCTCCTGAAC CAACCGCACC ACAGAGTTTC 
CTCTATGCGC ACTGCAAATC ACCTACACCC TCTGGCTCCG CCACTGCTCC ATCTCCCCCT 
TCACAACACA TCACAACCAA CCCAGAGGAC AGCGCAGCAC GCTCGTCTAC TCCCTCAACC 
TCCTCTTACT CCTCATCCGC CTCACAACGG CTCCACAACC GCTCTTTCAA CTCCCACCGC 
CTCATACAGT GCGACACCCT TGCACGCCGT CGGACAAAGG CGGACAAAGC CAAAGCTCCC 
TCAGTGGACC TTTCCGAACT CTCGAGCTCC TCCGCCGCAG AGGAGGACCG CCAGTCAGGT 
GCCAATCAAA TCCCTGACTC CTCTCCGTCT CCTTCTTCAA AAGCCAAAGG GTTTAGCATA 
AGAAACGAGT GTACGCCAAT ATCCATGGTT GGTCCCGGAA GTAAGGGGTG TGTGTTGTCC 
CCCGGGCAAG GATCCCTGGC GGATCAGGTA CGTGCTCGTC TAATGGGCTC GGCGGATGAC 
CTGTGCAGCG TTGGCCTGAG AAAGCAGCTC TCGCCGGAGA CGCGCCGGAA GAGACGCGCT 
TGGAGGAGAC ACACAGTGGT CGTGTCCCCT ACGGATTGTT CAGACAAAAA GCCAACGGTC 
CAAAGCACCA AACCTCTAGC TTCACCCTCC CCTGTTGAAA AGCAGGATGT CAGAGAGTCC 
CACCCAGAGT GCTCAGAAAC TGAAGCCCCT ACTGCACGTC GGGCCGTGCC TACCTCGCGC 
TTTCGTGATT TCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap23a ( XM_021474705.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330380.1 Danio rerio Rho GTPase activating protein 23a (arhgap23a), transcript variant X4, mRNA.

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CloneID	ODa48128
Clone ID Related Accession (Same CDS sequence)	XM_009299681.2 , XM_021474705.1
Accession Version	XM_021474705.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	rho GTPase-activating protein 23 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGG CAAAAGGGCG GAGAGATGGG CTCTCTTCAT CCAGTGAGAA CCCTCGGCCG 
GTGGTCCGGA GCGGGACAGA TGGGCTGGGC GTGTCCTGGC AGGGTCCACG CACGCTGGTG 
CTCCAAAAGA ACTCTCAGGG CTTCGGCTTT ACTCTGCGTC ACTTCATCGT TTACCCGCCA 
GAGTCCGCTC TGCACACCAC TCTCAAGGAT GAAGAGAACG GGAATGGAAA GGGGCTTCAA 
CGAAGTCGTC TGGAGCCCAT GGACACAATT TTTGTGAAGA ATGTGAGGGA AAAAGGCCCC 
GCTCACCTGG CTGGCCTGTG TACAGGAGAC AGGCTGGTTA AAGTGAATGG GGAGAGCGTG 
CTGGGTAAGA CGTACTCGCA GGTCATTGCA CTGATTCAGA ACAGTGAGAG TGTACTGGAG 
CTCTCCATTA TGCCAAAGGA TGAGGACGTG CTTCAGCTGG CATATTCCCA TGATGCCTAC 
CTAAGGGGGA ACAATCCATA TACAGGAAAG GCCCAAAATC TTCCTCCACC ACCCCCTGTT 
CTCTACCCTC CTCGTACAAA ATCACAGTCT CCTGCAGGGT TCAGCCCCTG CCCTCCTCCA 
GCTTCTTCAC ACATGGGGCA AAACCAGCTG GACAACTGGA GCCGCTGGCC CGGCTCTTCT 
GCTGGTCCCT CTCCACCCCT TGACAACCGG ACTGTGGCCT CCAGCAATGT TGTCTGGGCA 
GCCGAGGGCA GGAATTGTGA GCCGGGTGGT GTTGGTCACA GTAGCCCTGC ACACCGAACA 
GAGGAGATCC AGTATGGAAT GACTGACAGA GGACAGCAGC CAACTGGACA AATGAGAGGA 
CGCTCATACT CATCTTCTTC CTCCTCAGGA GGAATGATGA GCCCTTTGCA TCATGGTCAT 
CTTGCCAATC ACAACATGGG TACTGCCATG GGGCACACTG GAGGTCGTAA GGGAAGCCCA 
CCTTGGGCCA GCCCTTCTCA GCCAACATCA ACACCTTCAT CCAGCCGCAA CGAACGAACC 
CAACAAGCCC TTTCCAACTG GTACTACAGC CAGGTCCCAG GCCGTGAGCG AGAACGAGAA 
CGAGAGCGGG AGCGATCTAG TTCTCGCTCA ATGCACCCAC GCCACAGGAG TTTCTCACAA 
GACCGTCTGG GAGAGCTTGG GAAGGCCAGG CGTTCTCAAC AAGCAGGTTT TCCCCATAGT 
GCATCGCAAG ACACGTTGCT GCTGCTTCAG CAGAGTGCTG CAGCATATGC TGATCCTTAC 
TGGAACCATG GAGACTGGCG GGGTGAGCAT ATGTATGGTC AGCACCCATC TGTGGTGGCC 
TATGGGCGAA AGTCCTCCGA AAATCTTCTA GCAAGCCGGT ACGGACACTC GGGACGCTCA 
CTGGAAAATC TCGACAGAGC TGTGGGGATA CTCTCACCAA GGGTTGAAAG ATCTGCTTGG 
GTTCAACAAC CTCCAAATAG GACTGAAGTC TATGCAAGAC ATGCTGGGCA TTTCGGCCAA 
CATATAGAGT CACCCCACCA AATGCACCAC ACACCCCAAC ACCACCAGAA TCTCACACAT 
GCAACACACC AACACTCATC TCAAATCCCA CAACCTCAGC AGGCAGCTCC AATGGCAAGA 
CGTTTGCCTT CTTGCCAGAG TCTAGATGAG GAGCCGATTG GCTATCGTAG CTACAGTCCC 
TCATTCAATA GGAAGACTGG CCGTATCTTG CAGCAGGCCC AGTCATTTAG AGATCCCTCT 
TACACAGGTC CACACCTTTG GACTCCAACA TCCAAAACCA GTCCCCCAGA GGGGCATCCA 
CCCTCAGCCA CTGCTTCCCC AGCCCCACCA ACCACTTCCT CTTCAACTAC GATTTCCACA 
CCATCTGAAG GACAAGACAG AACTTACCGA CCCACTAATC ATGACAGAGG GGCAGGGGAA 
GCGACAAGTG AAGCCCAGAC ACAGGAAGTG GTTCTGAGGC AGAAACCACC TACCGGCCGA 
CGAGGTGCAA ACGCCCTTCG ACACCCACAT TATGCAATAG CACTTGACTC CAGTGACCCT 
CTACTCTTTA CCACTGACCC TACGGAGCCC ACTGCCAAGG CAGAAAGTGC TATTTCTCAT 
CGCCGCACAG ATGTTAACCT TCCTCCACTA TCTATTGAGG ATGACTCCCT TGCATCAATT 
CCATTCATCG ATGAGCCGAC CAGTCCAAGT GCAGATCTGC GGGCACGTCA TGTCCCCGCC 
TCCTCCGTCG TGTCCAGTGG CGGTATAAAC TCCGCCCCTG CTGTGATCAC AAGCCCCGCC 
TCCCCAACCT TCACCTTTCC CCTCAACCGC CTTTTCTCCC ACAACTGCAG TGATCCTAAA 
TCTAATCGTC GCTCCTCGTA CCTGTTGGCC ATCACCACTG AACGCTCCAA GTCATGTGAT 
GAGGGGCTTA ACGCATATAG AGAAGAGGGC CGGGTCTTCT CGAGGCTTCC AAAAAGAGTC 
AAGAGCTTCT TTACTGAAGG GTCTCTGGAC AGTCTGGGTG CTGCTGAAGA GGCTCGCTCT 
AAACGACACT CTACCTCTGA GCTGGGCAAC ATCAGCTACA GTGACGTGAA GAGAGAAGGC 
TGGCTCCACT TCAAACAGAT CCACACGGAG AAGGGCAAGA AGGTTGGCAG TGCAATCTGG 
CCATGGAGAC GGGTGTTCTC TGTGCTTAGG ACCCATTCTC TGTTTCTTTA TAAAGACAAG 
CGAGAGGCAG TGCTTAAAGG GGCGCAATTA GGGGGCGGGG CTGAAGACGA GCAGCCAATC 
AGCATCCGGG GCTGCCTTGT GGATATAGCA TACAGTGAAA CGAAGCGCAA GCACGCGCTG 
AGATTGACCA CGCAGGACTT CTGCGAGTAC CTGCTGCAGG CGGAGGACAG AGAGGACATG 
CTGGAGTGGA TCCGAGTCAT CAGGGACAAC AGCAAGACCG ACAACGAGGA ATTGGGTTTC 
TCACGACAAG CTCTCATCAA TAAAAAGCTT AACGACTACA GGAAGCAAAG TCCAACGGGT 
AACAAACCAG ACTCCTCACC CAGAGTGCAT CGAATGACCT TCCTCCTCAG CAAGCCAGAC 
AACAGCGCAC CTCCACGCTC CCCAAAACAC GAGGCCAAAG ATGAGAGCAG CCCCCCAAAA 
TCTCCCTGGG GTATAAACAT AATGAAGAAG AGCAAGAAGG CCGGTCCTAA AGCTTTTGGC 
GTCCGTCTAG AGGACTGCCA GCCTGCAGTA AACAACAAGT TCATTCCTCA GATTGTGGAG 
ATCTGCTGTG GGCTGGTGGA GGACATGGGT CTGGAGTATA CAGGGATCTA CAGAGTGCCA 
GGAAACAATG CTGTAGTCTC CAGTCTGCAG GAGCAGCTCA ACAAAGGAGT AGACATTAAC 
ATCACTGAGG AGAAATGGCA GGACCTGAAT GTGGTGAGCA GCCTGCTGAA GTCCTTCTTT 
CGTAAACTCC CTGAACCCCT CTTCACTGAT GATAAATATA ATGACTTTAT CGACGCCAAT 
CGGATGGAGA ACGCCAGCGA TAGGCTGAGG ACAATGAAGA AATTGATTCG GGACCTACCA 
GATCATTACT TTCACACTCT GAAATTTTTA GTTGGACACC TAAAGACGGT GGCTGACCAC 
TCTGAGAAAA ACAAAATGGA GCCGCGTAAT TTGGCTCTGG TGTTTGGCCC CACTCTTGTT 
CGAACTTCAG AGGACAACAT GACAGACATG GTCACCCACA TGCCAGACCG CTACAAGATT 
GTGGAGACAC TCATTCAGCA TTACACCTGG TTTTTCTGCG AAGAACAGGA TAAGGATGAA 
AAGACCCCAG TGGACACAGA GGATGTCCAG CCTGCCCCAA ACATTGATCA TCTGCTCTCC 
AACATTGGCC GCACAGGCAT GCTGGGTGAG GCTTCAGACT CAACCAACAG TGATTCTGCA 
AAATCTAAGG GGTCATGGGG ATCAAAACGT GACTTTACTG CCAAGGATAT CCTTACATTA 
TCAATTATGT CGGCTGTGAC CCGCAAACGG CGGAAGCGGC ACAACGGCCG TCTCCTCGGC 
AGCAGCACAG ACGACGATTC TGAGCATGAA CCCATTAAAT CCTGCATCTC CGAGAAAGAC 
GATCTGCAAG AGATGGGGCA GGTTGCATCC TTCTGTGCCC CACGAGCAGA GGGAGAGGAG 
GATGACGATG AAGAGGAGGA AGATGATGAA GAGGAGGAGG AGGGGGCTGA GAGACAGAAG 
GCACAAGAGC AGAAGGAAAC AGTAGTCCCC AGCATTTCCT CAACAGAGGA AGTGACATCA 
ACAGTGCTGC TGAGCGAAGA GGAAGAGCAG AAGTCTGAAG TGAAAGGAGG ACGCAGCTGG 
AGATTAGATG ATGCTCGGTC TATTGTGTCA GGCTACTCTA CTCTGTCCAC TTTGGGGCGG 
AGCTTAGCTT CAGAGGGGCG GGGCGAGGAT GCAGATGATG AGCATAGTGA ACTAGTGAGT 
GAAACTGATA ATGAAAGCGG GTTTGCCTCA CGCTCCCTCA CCCAAGAAAG GCCAGAAAAA 
CCAAACCGTT CCAACCAGAA CTCTCATGCG TCTCCTGAAC CAACCGCACC ACAGAGTTTC 
CTCTATGCGC ACTGCAAATC ACCTACACCC TCTGGCTCCG CCACTGCTCC ATCTCCCCCT 
TCACAACACA TCACAACCAA CCCAGAGGAC AGCGCAGCAC GCTCGTCTAC TCCCTCAACC 
TCCTCTTACT CCTCATCCGC CTCACAACGG CTCCACAACC GCTCTTTCAA CTCCCACCGC 
CTCATACAGT GCGACACCCT TGCACGCCGT CGGACAAAGG CGGACAAAGC CAAAGCTCCC 
TCAGTGGACC TTTCCGAACT CTCGAGCTCC TCCGCCGCAG AGGAGGACCG CCAGTCAGGT 
GCCAATCAAA TCCCTGACTC CTCTCCGTCT CCTTCTTCAA AAGCCAAAGG GTTTAGCATA 
AGAAACGAGT GTACGCCAAT ATCCATGGTT GGTCCCGGAA GTAAGGGGTG TGTGTTGTCC 
CCCGGGCAAG GATCCCTGGC GGATCAGGTA CGTGCTCGTC TAATGGGCTC GGCGGATGAC 
CTGTGCAGCG TTGGCCTGAG AAAGCAGCTC TCGCCGGAGA CGCGCCGGAA GAGACGCGCT 
TGGAGGAGAC ACACAGTGGT CGTGTCCCCT ACGGATTGTT CAGACAAAAA GCCAACGGTC 
CAAAGCACCA AACCTCTAGC TTCACCCTCC CCTGTTGAAA AGCAGGATGT CAGAGAGTCC 
CACCCAGAGT GCTCAGAAAC TGAAGCCCCT ACTGCACGTC GGGCCGTGCC TACCTCGCGC 
TTTCGTGATT TCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330380.1
CDS	553..5850
Translation	MEQAKGRRDGLSSSSENPRPVVRSGTDGLGVSWQGPRTLVLQKNSQGFGFTLRHFIVYPPESALHTTLKDEENGNGKGLQRSRLEPMDTIFVKNVREKGPAHLAGLCTGDRLVKVNGESVLGKTYSQVIALIQNSESVLELSIMPKDEDVLQLAYSHDAYLRGNNPYTGKAQNLPPPPPVLYPPRTKSQSPAGFSPCPPPASSHMGQNQLDNWSRWPGSSAGPSPPLDNRTVASSNVVWAAEGRNCEPGGVGHSSPAHRTEEIQYGMTDRGQQPTGQMRGRSYSSSSSSGGMMSPLHHGHLANHNMGTAMGHTGGRKGSPPWASPSQPTSTPSSSRNERTQQALSNWYYSQVPGRERERERERERSSSRSMHPRHRSFSQDRLGELGKARRSQQAGFPHSASQDTLLLLQQSAAAYADPYWNHGDWRGEHMYGQHPSVVAYGRKSSENLLASRYGHSGRSLENLDRAVGILSPRVERSAWVQQPPNRTEVYARHAGHFGQHIESPHQMHHTPQHHQNLTHATHQHSSQIPQPQQAAPMARRLPSCQSLDEEPIGYRSYSPSFNRKTGRILQQAQSFRDPSYTGPHLWTPTSKTSPPEGHPPSATASPAPPTTSSSTTISTPSEGQDRTYRPTNHDRGAGEATSEAQTQEVVLRQKPPTGRRGANALRHPHYAIALDSSDPLLFTTDPTEPTAKAESAISHRRTDVNLPPLSIEDDSLASIPFIDEPTSPSADLRARHVPASSVVSSGGINSAPAVITSPASPTFTFPLNRLFSHNCSDPKSNRRSSYLLAITTERSKSCDEGLNAYREEGRVFSRLPKRVKSFFTEGSLDSLGAAEEARSKRHSTSELGNISYSDVKREGWLHFKQIHTEKGKKVGSAIWPWRRVFSVLRTHSLFLYKDKREAVLKGAQLGGGAEDEQPISIRGCLVDIAYSETKRKHALRLTTQDFCEYLLQAEDREDMLEWIRVIRDNSKTDNEELGFSRQALINKKLNDYRKQSPTGNKPDSSPRVHRMTFLLSKPDNSAPPRSPKHEAKDESSPPKSPWGINIMKKSKKAGPKAFGVRLEDCQPAVNNKFIPQIVEICCGLVEDMGLEYTGIYRVPGNNAVVSSLQEQLNKGVDINITEEKWQDLNVVSSLLKSFFRKLPEPLFTDDKYNDFIDANRMENASDRLRTMKKLIRDLPDHYFHTLKFLVGHLKTVADHSEKNKMEPRNLALVFGPTLVRTSEDNMTDMVTHMPDRYKIVETLIQHYTWFFCEEQDKDEKTPVDTEDVQPAPNIDHLLSNIGRTGMLGEASDSTNSDSAKSKGSWGSKRDFTAKDILTLSIMSAVTRKRRKRHNGRLLGSSTDDDSEHEPIKSCISEKDDLQEMGQVASFCAPRAEGEEDDDEEEEDDEEEEEGAERQKAQEQKETVVPSISSTEEVTSTVLLSEEEEQKSEVKGGRSWRLDDARSIVSGYSTLSTLGRSLASEGRGEDADDEHSELVSETDNESGFASRSLTQERPEKPNRSNQNSHASPEPTAPQSFLYAHCKSPTPSGSATAPSPPSQHITTNPEDSAARSSTPSTSSYSSSASQRLHNRSFNSHRLIQCDTLARRRTKADKAKAPSVDLSELSSSSAAEEDRQSGANQIPDSSPSPSSKAKGFSIRNECTPISMVGPGSKGCVLSPGQGSLADQVRARLMGSADDLCSVGLRKQLSPETRRKRRAWRRHTVVVSPTDCSDKKPTVQSTKPLASPSPVEKQDVRESHPECSETEAPTARRAVPTSRFRDFL

Target ORF information:

RefSeq Version	XM_021474705.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Rho GTPase activating protein 23a (arhgap23a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474705.1

ORF Insert Sequence:

ATGGAGCAGG CAAAAGGGCG GAGAGATGGG CTCTCTTCAT CCAGTGAGAA CCCTCGGCCG 
GTGGTCCGGA GCGGGACAGA TGGGCTGGGC GTGTCCTGGC AGGGTCCACG CACGCTGGTG 
CTCCAAAAGA ACTCTCAGGG CTTCGGCTTT ACTCTGCGTC ACTTCATCGT TTACCCGCCA 
GAGTCCGCTC TGCACACCAC TCTCAAGGAT GAAGAGAACG GGAATGGAAA GGGGCTTCAA 
CGAAGTCGTC TGGAGCCCAT GGACACAATT TTTGTGAAGA ATGTGAGGGA AAAAGGCCCC 
GCTCACCTGG CTGGCCTGTG TACAGGAGAC AGGCTGGTTA AAGTGAATGG GGAGAGCGTG 
CTGGGTAAGA CGTACTCGCA GGTCATTGCA CTGATTCAGA ACAGTGAGAG TGTACTGGAG 
CTCTCCATTA TGCCAAAGGA TGAGGACGTG CTTCAGCTGG CATATTCCCA TGATGCCTAC 
CTAAGGGGGA ACAATCCATA TACAGGAAAG GCCCAAAATC TTCCTCCACC ACCCCCTGTT 
CTCTACCCTC CTCGTACAAA ATCACAGTCT CCTGCAGGGT TCAGCCCCTG CCCTCCTCCA 
GCTTCTTCAC ACATGGGGCA AAACCAGCTG GACAACTGGA GCCGCTGGCC CGGCTCTTCT 
GCTGGTCCCT CTCCACCCCT TGACAACCGG ACTGTGGCCT CCAGCAATGT TGTCTGGGCA 
GCCGAGGGCA GGAATTGTGA GCCGGGTGGT GTTGGTCACA GTAGCCCTGC ACACCGAACA 
GAGGAGATCC AGTATGGAAT GACTGACAGA GGACAGCAGC CAACTGGACA AATGAGAGGA 
CGCTCATACT CATCTTCTTC CTCCTCAGGA GGAATGATGA GCCCTTTGCA TCATGGTCAT 
CTTGCCAATC ACAACATGGG TACTGCCATG GGGCACACTG GAGGTCGTAA GGGAAGCCCA 
CCTTGGGCCA GCCCTTCTCA GCCAACATCA ACACCTTCAT CCAGCCGCAA CGAACGAACC 
CAACAAGCCC TTTCCAACTG GTACTACAGC CAGGTCCCAG GCCGTGAGCG AGAACGAGAA 
CGAGAGCGGG AGCGATCTAG TTCTCGCTCA ATGCACCCAC GCCACAGGAG TTTCTCACAA 
GACCGTCTGG GAGAGCTTGG GAAGGCCAGG CGTTCTCAAC AAGCAGGTTT TCCCCATAGT 
GCATCGCAAG ACACGTTGCT GCTGCTTCAG CAGAGTGCTG CAGCATATGC TGATCCTTAC 
TGGAACCATG GAGACTGGCG GGGTGAGCAT ATGTATGGTC AGCACCCATC TGTGGTGGCC 
TATGGGCGAA AGTCCTCCGA AAATCTTCTA GCAAGCCGGT ACGGACACTC GGGACGCTCA 
CTGGAAAATC TCGACAGAGC TGTGGGGATA CTCTCACCAA GGGTTGAAAG ATCTGCTTGG 
GTTCAACAAC CTCCAAATAG GACTGAAGTC TATGCAAGAC ATGCTGGGCA TTTCGGCCAA 
CATATAGAGT CACCCCACCA AATGCACCAC ACACCCCAAC ACCACCAGAA TCTCACACAT 
GCAACACACC AACACTCATC TCAAATCCCA CAACCTCAGC AGGCAGCTCC AATGGCAAGA 
CGTTTGCCTT CTTGCCAGAG TCTAGATGAG GAGCCGATTG GCTATCGTAG CTACAGTCCC 
TCATTCAATA GGAAGACTGG CCGTATCTTG CAGCAGGCCC AGTCATTTAG AGATCCCTCT 
TACACAGGTC CACACCTTTG GACTCCAACA TCCAAAACCA GTCCCCCAGA GGGGCATCCA 
CCCTCAGCCA CTGCTTCCCC AGCCCCACCA ACCACTTCCT CTTCAACTAC GATTTCCACA 
CCATCTGAAG GACAAGACAG AACTTACCGA CCCACTAATC ATGACAGAGG GGCAGGGGAA 
GCGACAAGTG AAGCCCAGAC ACAGGAAGTG GTTCTGAGGC AGAAACCACC TACCGGCCGA 
CGAGGTGCAA ACGCCCTTCG ACACCCACAT TATGCAATAG CACTTGACTC CAGTGACCCT 
CTACTCTTTA CCACTGACCC TACGGAGCCC ACTGCCAAGG CAGAAAGTGC TATTTCTCAT 
CGCCGCACAG ATGTTAACCT TCCTCCACTA TCTATTGAGG ATGACTCCCT TGCATCAATT 
CCATTCATCG ATGAGCCGAC CAGTCCAAGT GCAGATCTGC GGGCACGTCA TGTCCCCGCC 
TCCTCCGTCG TGTCCAGTGG CGGTATAAAC TCCGCCCCTG CTGTGATCAC AAGCCCCGCC 
TCCCCAACCT TCACCTTTCC CCTCAACCGC CTTTTCTCCC ACAACTGCAG TGATCCTAAA 
TCTAATCGTC GCTCCTCGTA CCTGTTGGCC ATCACCACTG AACGCTCCAA GTCATGTGAT 
GAGGGGCTTA ACGCATATAG AGAAGAGGGC CGGGTCTTCT CGAGGCTTCC AAAAAGAGTC 
AAGAGCTTCT TTACTGAAGG GTCTCTGGAC AGTCTGGGTG CTGCTGAAGA GGCTCGCTCT 
AAACGACACT CTACCTCTGA GCTGGGCAAC ATCAGCTACA GTGACGTGAA GAGAGAAGGC 
TGGCTCCACT TCAAACAGAT CCACACGGAG AAGGGCAAGA AGGTTGGCAG TGCAATCTGG 
CCATGGAGAC GGGTGTTCTC TGTGCTTAGG ACCCATTCTC TGTTTCTTTA TAAAGACAAG 
CGAGAGGCAG TGCTTAAAGG GGCGCAATTA GGGGGCGGGG CTGAAGACGA GCAGCCAATC 
AGCATCCGGG GCTGCCTTGT GGATATAGCA TACAGTGAAA CGAAGCGCAA GCACGCGCTG 
AGATTGACCA CGCAGGACTT CTGCGAGTAC CTGCTGCAGG CGGAGGACAG AGAGGACATG 
CTGGAGTGGA TCCGAGTCAT CAGGGACAAC AGCAAGACCG ACAACGAGGA ATTGGGTTTC 
TCACGACAAG CTCTCATCAA TAAAAAGCTT AACGACTACA GGAAGCAAAG TCCAACGGGT 
AACAAACCAG ACTCCTCACC CAGAGTGCAT CGAATGACCT TCCTCCTCAG CAAGCCAGAC 
AACAGCGCAC CTCCACGCTC CCCAAAACAC GAGGCCAAAG ATGAGAGCAG CCCCCCAAAA 
TCTCCCTGGG GTATAAACAT AATGAAGAAG AGCAAGAAGG CCGGTCCTAA AGCTTTTGGC 
GTCCGTCTAG AGGACTGCCA GCCTGCAGTA AACAACAAGT TCATTCCTCA GATTGTGGAG 
ATCTGCTGTG GGCTGGTGGA GGACATGGGT CTGGAGTATA CAGGGATCTA CAGAGTGCCA 
GGAAACAATG CTGTAGTCTC CAGTCTGCAG GAGCAGCTCA ACAAAGGAGT AGACATTAAC 
ATCACTGAGG AGAAATGGCA GGACCTGAAT GTGGTGAGCA GCCTGCTGAA GTCCTTCTTT 
CGTAAACTCC CTGAACCCCT CTTCACTGAT GATAAATATA ATGACTTTAT CGACGCCAAT 
CGGATGGAGA ACGCCAGCGA TAGGCTGAGG ACAATGAAGA AATTGATTCG GGACCTACCA 
GATCATTACT TTCACACTCT GAAATTTTTA GTTGGACACC TAAAGACGGT GGCTGACCAC 
TCTGAGAAAA ACAAAATGGA GCCGCGTAAT TTGGCTCTGG TGTTTGGCCC CACTCTTGTT 
CGAACTTCAG AGGACAACAT GACAGACATG GTCACCCACA TGCCAGACCG CTACAAGATT 
GTGGAGACAC TCATTCAGCA TTACACCTGG TTTTTCTGCG AAGAACAGGA TAAGGATGAA 
AAGACCCCAG TGGACACAGA GGATGTCCAG CCTGCCCCAA ACATTGATCA TCTGCTCTCC 
AACATTGGCC GCACAGGCAT GCTGGGTGAG GCTTCAGACT CAACCAACAG TGATTCTGCA 
AAATCTAAGG GGTCATGGGG ATCAAAACGT GACTTTACTG CCAAGGATAT CCTTACATTA 
TCAATTATGT CGGCTGTGAC CCGCAAACGG CGGAAGCGGC ACAACGGCCG TCTCCTCGGC 
AGCAGCACAG ACGACGATTC TGAGCATGAA CCCATTAAAT CCTGCATCTC CGAGAAAGAC 
GATCTGCAAG AGATGGGGCA GGTTGCATCC TTCTGTGCCC CACGAGCAGA GGGAGAGGAG 
GATGACGATG AAGAGGAGGA AGATGATGAA GAGGAGGAGG AGGGGGCTGA GAGACAGAAG 
GCACAAGAGC AGAAGGAAAC AGTAGTCCCC AGCATTTCCT CAACAGAGGA AGTGACATCA 
ACAGTGCTGC TGAGCGAAGA GGAAGAGCAG AAGTCTGAAG TGAAAGGAGG ACGCAGCTGG 
AGATTAGATG ATGCTCGGTC TATTGTGTCA GGCTACTCTA CTCTGTCCAC TTTGGGGCGG 
AGCTTAGCTT CAGAGGGGCG GGGCGAGGAT GCAGATGATG AGCATAGTGA ACTAGTGAGT 
GAAACTGATA ATGAAAGCGG GTTTGCCTCA CGCTCCCTCA CCCAAGAAAG GCCAGAAAAA 
CCAAACCGTT CCAACCAGAA CTCTCATGCG TCTCCTGAAC CAACCGCACC ACAGAGTTTC 
CTCTATGCGC ACTGCAAATC ACCTACACCC TCTGGCTCCG CCACTGCTCC ATCTCCCCCT 
TCACAACACA TCACAACCAA CCCAGAGGAC AGCGCAGCAC GCTCGTCTAC TCCCTCAACC 
TCCTCTTACT CCTCATCCGC CTCACAACGG CTCCACAACC GCTCTTTCAA CTCCCACCGC 
CTCATACAGT GCGACACCCT TGCACGCCGT CGGACAAAGG CGGACAAAGC CAAAGCTCCC 
TCAGTGGACC TTTCCGAACT CTCGAGCTCC TCCGCCGCAG AGGAGGACCG CCAGTCAGGT 
GCCAATCAAA TCCCTGACTC CTCTCCGTCT CCTTCTTCAA AAGCCAAAGG GTTTAGCATA 
AGAAACGAGT GTACGCCAAT ATCCATGGTT GGTCCCGGAA GTAAGGGGTG TGTGTTGTCC 
CCCGGGCAAG GATCCCTGGC GGATCAGGTA CGTGCTCGTC TAATGGGCTC GGCGGATGAC 
CTGTGCAGCG TTGGCCTGAG AAAGCAGCTC TCGCCGGAGA CGCGCCGGAA GAGACGCGCT 
TGGAGGAGAC ACACAGTGGT CGTGTCCCCT ACGGATTGTT CAGACAAAAA GCCAACGGTC 
CAAAGCACCA AACCTCTAGC TTCACCCTCC CCTGTTGAAA AGCAGGATGT CAGAGAGTCC 
CACCCAGAGT GCTCAGAAAC TGAAGCCCCT ACTGCACGTC GGGCCGTGCC TACCTCGCGC 
TTTCGTGATT TCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	arhgap23a
Entrez Gene ID	100006233
Full Name	Rho GTPase activating protein 23a
Synonyms	sb:eu857
General protein information	Preferred Names Rho GTPase activating protein 23a Names Rho GTPase activating protein 23a rho GTPase-activating protein 23
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Rattus norvegicus (Norway rat)	Arhgap23	XP_220918.6
Homo sapiens (human)	ARHGAP23	NP_001186346.1
Pan troglodytes (chimpanzee)	ARHGAP23	XP_003315576.2
	ARHGAP23	XP_001082710.2
Canis lupus familiaris (dog)	ARHGAP23	XP_005624648.1
Mus musculus (house mouse)	Arhgap23	NP_067468.2
Bos taurus (cattle)	ARHGAP23	XP_002695993.3
Gallus gallus (chicken)	LOC101750894	XP_004950782.1
Xenopus tropicalis (tropical clawed frog)	arhgap23	XP_002940202.2
Danio rerio (zebrafish)	LOC100006233	XP_005164231.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

arhgap23a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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