rhoab cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	rhoab
Entrez Gene ID	100006041
Full Name	ras homolog gene family, member Ab
Synonyms	arha,rhoa,wu:fj42e08,zgc:92206
General protein information	Preferred Names ras homolog gene family, member Ab Names rho-related GTP-binding protein RhoA-B small GTPase RhoA
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005166980.3	XP_005167037.1	rho-related GTP-binding protein RhoA-B isoform X1
NM_212749.2	NP_997914.2	rho-related GTP-binding protein RhoA-B
XM_021478160.1	XP_021333835.1	rho-related GTP-binding protein RhoA-B isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	RHOA	NP_001655.1
Canis lupus familiaris (dog)	RHOA	NP_001003273.2
Mus musculus (house mouse)	Rhoa	NP_058082.2
Gallus gallus (chicken)	RHOA	NP_990035.1
Eremothecium gossypii	AGOS_ABR183W	NP_983131.1
Schizosaccharomyces pombe (fission yeast)	rho1	NP_594490.1
Neurospora crassa	NCU01484	XP_956761.2
Pan troglodytes (chimpanzee)	RHOA	XP_003950161.1
Bos taurus (cattle)	RHOA	NP_788818.1
Rattus norvegicus (Norway rat)	Rhoa	NP_476473.1
Danio rerio (zebrafish)	rhoab	NP_997914.2
Kluyveromyces lactis	KLLA0B10626g	XP_452002.1
Magnaporthe oryzae (rice blast fungus)	MGG_07176	XP_003715379.1
Schizosaccharomyces pombe (fission yeast)	rho5	NP_593631.1
Saccharomyces cerevisiae (baker's yeast)	RHO1	NP_015491.1
Xenopus tropicalis (tropical clawed frog)	rhoa.2	NP_001016648.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Tissue Extract Fractions from Starfish Undergoing Regeneration Promote Wound Healing and Lower Jaw Blastema Regeneration of Zebrafish.
Dai Y, Prithiviraj N, Gan J, Zhang XA, Yan J
Scientific reports638693(2016 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Cell movement defects are caused by defective RhoA regulation.
Title: Pair-wise regulation of convergence and extension cell movements by four phosphatases via RhoA.

results provide first evidence that RhoA in vivo acts downstream of Wnt5 and Wnt11 to effect, without affecting cell fates, the convergence and extension movements in zebrafish embryos; it elicits such effect via both effectors, Rho kinase and Dia
Title: RhoA acts downstream of Wnt5 and Wnt11 to regulate convergence and extension movements by involving effectors Rho kinase and Diaphanous: use of zebrafish as an in vivo model for GTPase signaling.

The following rhoab gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rhoab cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa31396	NM_212749.2 Latest version!	Danio rerio ras homolog gene family, member Ab (rhoab), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
ODa14174	XM_005166980.3 Latest version!	Danio rerio ras homolog gene family, member Ab (rhoab), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
ODa14174	XM_021478160.1 Latest version!	Danio rerio ras homolog gene family, member Ab (rhoab), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rhoab ( NM_212749.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_997914.2 Danio rerio ras homolog gene family, member Ab (rhoab), mRNA.

Price & Availability↑

CloneID	ODa31396
Clone ID Related Accession (Same CDS sequence)	NM_212749.2
Accession Version	NM_212749.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-23
Organism	Danio rerio(zebrafish)
Product	rho-related GTP-binding protein RhoA-B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC075938.1. On or before Jul 6, 2005 this sequence version replaced XM_694659.1, NM_212749.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC075938.1, BC152258.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGCAA TTCGCAAGAA GCTGGTGATT GTAGGAGATG GAGCATGCGG GAAAACATGT 
TTGCTTATTG TGTTCAGTAA AGACCAGTTT CCTGAAGTCT ACGTACCAAC AGTGTTTGAA 
AACTATGTTG CCGACATTGA AGTTGACAGC AAACAGGTGG AACTCGCTCT GTGGGATACT 
GCAGGGCAGG AGGACTACGA CAGGCTTCGT CCTCTTTCAT ACCCAGACAC CGATGTCATT 
CTTATGTGTT TCTCTATTGA CAGTCCTGAC AGTTTAGAGA ATATTCCGGA GAAGTGGACG 
CCTGAGGTCA AACACTTTTG TCCAAACGTT CCAATCATCC TTGTGGGAAA CAAAAAAGAC 
CTGCGCAATG ATGAACACAC ACGCAGGGAA CTGACCAAGA TGAAGCAGGA GCCAGTTAAA 
GCAGAGGAAG GGAGAGACAT GGCCAATCGA ATTGGAGCAT TTGGTTATAT GGAATGTTCT 
GCCAAAACAA AAGACGGTGT TCGGGAGGTG TTTGAAATGG CCACTAGAGC GGCGCTGCAG 
GCACGCAGGG GAAAGAAGAG CAACAAATGC TGTCTGCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997914.2
CDS	54..635
Translation	MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDSKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRNDEHTRRELTKMKQEPVKAEEGRDMANRIGAFGYMECSAKTKDGVREVFEMATRAALQARRGKKSNKCCLL

Target ORF information:

RefSeq Version	NM_212749.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ras homolog gene family, member Ab (rhoab), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212749.2

ORF Insert Sequence:

ATGGCAGCAA TTCGCAAGAA GCTGGTGATT GTAGGAGATG GAGCATGCGG GAAAACATGT 
TTGCTTATTG TGTTCAGTAA AGACCAGTTT CCTGAAGTCT ACGTACCAAC AGTGTTTGAA 
AACTATGTTG CCGACATTGA AGTTGACAGC AAACAGGTGG AACTCGCTCT GTGGGATACT 
GCAGGGCAGG AGGACTACGA CAGGCTTCGT CCTCTTTCAT ACCCAGACAC CGATGTCATT 
CTTATGTGTT TCTCTATTGA CAGTCCTGAC AGTTTAGAGA ATATTCCGGA GAAGTGGACG 
CCTGAGGTCA AACACTTTTG TCCAAACGTT CCAATCATCC TTGTGGGAAA CAAAAAAGAC 
CTGCGCAATG ATGAACACAC ACGCAGGGAA CTGACCAAGA TGAAGCAGGA GCCAGTTAAA 
GCAGAGGAAG GGAGAGACAT GGCCAATCGA ATTGGAGCAT TTGGTTATAT GGAATGTTCT 
GCCAAAACAA AAGACGGTGT TCGGGAGGTG TTTGAAATGG CCACTAGAGC GGCGCTGCAG 
GCACGCAGGG GAAAGAAGAG CAACAAATGC TGTCTGCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rhoab ( XM_005166980.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005167037.1 Danio rerio ras homolog gene family, member Ab (rhoab), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa14174
Clone ID Related Accession (Same CDS sequence)	XM_005166980.3 , XM_021478160.1
Accession Version	XM_005166980.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	rho-related GTP-binding protein RhoA-B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005166980.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAA TTCGCAAGAA GCTGGTGATT GTAGGAGATG GAGCATGCGG GAAAACATGT 
TTGCTTATTG TGTTCAGTAA AGACCAGTTT CCTGAAGTCT ACGTACCAAC AGTGTTTGAA 
AACTATGTTG CCGACATTGA AGTTGACAGC AAACAGGTGG AACTCGCTCT GTGGGATACT 
GCAGGGCAGG AGGACTACGA CAGGCTTCGT CCTCTTTCAT ACCCAGACAC CGATGTCATT 
CTTATGTGTT TCTCTATTGA CAGTCCTGAC AGTTTAGAGA ATATTCCGGA GAAGTGGACG 
CCTGAGGTCA AACATTTTTG TCCAAACGTT CCAATCATCC TTGTGGGAAA CAAAAAAGAC 
CTGCGCAATG ATGAACACAC ACGCAGGGAA CTGACCAAGA TGAAGCAGGA GCCAGTTAAA 
GCAGAGGAAG GGAGAGACAT GGCCAATCGA ATTGGAGCAT TTGGTTATAT GGAATGTTCT 
GCCAAAACAA AAGACGGTGT TCGGGAGGTG TTTGAAATGG CCACTAGAGC GGCGCTGCAG 
GCACGCAGGG GAAAGAAGAG CAACAAATGC TGTCTGCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167037.1
CDS	299..880
Translation	MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDSKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRNDEHTRRELTKMKQEPVKAEEGRDMANRIGAFGYMECSAKTKDGVREVFEMATRAALQARRGKKSNKCCLL

Target ORF information:

RefSeq Version	XM_005166980.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ras homolog gene family, member Ab (rhoab), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166980.3

ORF Insert Sequence:

ATGGCAGCAA TTCGCAAGAA GCTGGTGATT GTAGGAGATG GAGCATGCGG GAAAACATGT 
TTGCTTATTG TGTTCAGTAA AGACCAGTTT CCTGAAGTCT ACGTACCAAC AGTGTTTGAA 
AACTATGTTG CCGACATTGA AGTTGACAGC AAACAGGTGG AACTCGCTCT GTGGGATACT 
GCAGGGCAGG AGGACTACGA CAGGCTTCGT CCTCTTTCAT ACCCAGACAC CGATGTCATT 
CTTATGTGTT TCTCTATTGA CAGTCCTGAC AGTTTAGAGA ATATTCCGGA GAAGTGGACG 
CCTGAGGTCA AACATTTTTG TCCAAACGTT CCAATCATCC TTGTGGGAAA CAAAAAAGAC 
CTGCGCAATG ATGAACACAC ACGCAGGGAA CTGACCAAGA TGAAGCAGGA GCCAGTTAAA 
GCAGAGGAAG GGAGAGACAT GGCCAATCGA ATTGGAGCAT TTGGTTATAT GGAATGTTCT 
GCCAAAACAA AAGACGGTGT TCGGGAGGTG TTTGAAATGG CCACTAGAGC GGCGCTGCAG 
GCACGCAGGG GAAAGAAGAG CAACAAATGC TGTCTGCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rhoab ( XM_021478160.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333835.1 Danio rerio ras homolog gene family, member Ab (rhoab), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa14174
Clone ID Related Accession (Same CDS sequence)	XM_005166980.3 , XM_021478160.1
Accession Version	XM_021478160.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	rho-related GTP-binding protein RhoA-B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAA TTCGCAAGAA GCTGGTGATT GTAGGAGATG GAGCATGCGG GAAAACATGT 
TTGCTTATTG TGTTCAGTAA AGACCAGTTT CCTGAAGTCT ACGTACCAAC AGTGTTTGAA 
AACTATGTTG CCGACATTGA AGTTGACAGC AAACAGGTGG AACTCGCTCT GTGGGATACT 
GCAGGGCAGG AGGACTACGA CAGGCTTCGT CCTCTTTCAT ACCCAGACAC CGATGTCATT 
CTTATGTGTT TCTCTATTGA CAGTCCTGAC AGTTTAGAGA ATATTCCGGA GAAGTGGACG 
CCTGAGGTCA AACATTTTTG TCCAAACGTT CCAATCATCC TTGTGGGAAA CAAAAAAGAC 
CTGCGCAATG ATGAACACAC ACGCAGGGAA CTGACCAAGA TGAAGCAGGA GCCAGTTAAA 
GCAGAGGAAG GGAGAGACAT GGCCAATCGA ATTGGAGCAT TTGGTTATAT GGAATGTTCT 
GCCAAAACAA AAGACGGTGT TCGGGAGGTG TTTGAAATGG CCACTAGAGC GGCGCTGCAG 
GCACGCAGGG GAAAGAAGAG CAACAAATGC TGTCTGCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333835.1
CDS	181..762
Translation	MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDSKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRNDEHTRRELTKMKQEPVKAEEGRDMANRIGAFGYMECSAKTKDGVREVFEMATRAALQARRGKKSNKCCLL

Target ORF information:

RefSeq Version	XM_021478160.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ras homolog gene family, member Ab (rhoab), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478160.1

ORF Insert Sequence:

ATGGCAGCAA TTCGCAAGAA GCTGGTGATT GTAGGAGATG GAGCATGCGG GAAAACATGT 
TTGCTTATTG TGTTCAGTAA AGACCAGTTT CCTGAAGTCT ACGTACCAAC AGTGTTTGAA 
AACTATGTTG CCGACATTGA AGTTGACAGC AAACAGGTGG AACTCGCTCT GTGGGATACT 
GCAGGGCAGG AGGACTACGA CAGGCTTCGT CCTCTTTCAT ACCCAGACAC CGATGTCATT 
CTTATGTGTT TCTCTATTGA CAGTCCTGAC AGTTTAGAGA ATATTCCGGA GAAGTGGACG 
CCTGAGGTCA AACATTTTTG TCCAAACGTT CCAATCATCC TTGTGGGAAA CAAAAAAGAC 
CTGCGCAATG ATGAACACAC ACGCAGGGAA CTGACCAAGA TGAAGCAGGA GCCAGTTAAA 
GCAGAGGAAG GGAGAGACAT GGCCAATCGA ATTGGAGCAT TTGGTTATAT GGAATGTTCT 
GCCAAAACAA AAGACGGTGT TCGGGAGGTG TTTGAAATGG CCACTAGAGC GGCGCTGCAG 
GCACGCAGGG GAAAGAAGAG CAACAAATGC TGTCTGCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Tissue Extract Fractions from Starfish Undergoing Regeneration Promote Wound Healing and Lower Jaw Blastema Regeneration of Zebrafish.
Scientific reports638693(2016 Dec)
Dai Y,Prithiviraj N,Gan J,Zhang XA,Yan J

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rhoab cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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