grin1b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	grin1b
Entrez Gene ID	100005675
Full Name	glutamate receptor, ionotropic, N-methyl D-aspartate 1b
Synonyms	NMDAR1.2,si:rp71-1o1.1
General protein information	Preferred Names glutamate receptor, ionotropic, N-methyl D-aspartate 1b Names glutamate receptor, ionotropic, N-methyl D-aspartate 1b
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005171776.2	XP_005171833.1	glutamate receptor, ionotropic, N-methyl D-aspartate 1b isoform X1
NM_001144131.1	NP_001137603.1	glutamate receptor, ionotropic, N-methyl D-aspartate 1b precursor
XM_005171776.1	XP_005171833.1	glutamate receptor, ionotropic, N-methyl D-aspartate 1b isoform X1
XM_005171777.1	XP_005171834.1	glutamate receptor, ionotropic, N-methyl D-aspartate 1b isoform X2
XM_005171777.2	XP_005171834.1	glutamate receptor, ionotropic, N-methyl D-aspartate 1b isoform X2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	grin1b	NP_001137603.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001478	XP_321646.3
Caenorhabditis elegans (roundworm)	nmr-1	NP_495033.2
Xenopus tropicalis (tropical clawed frog)	grin1	NP_001072593.1
Homo sapiens (human)	GRIN1	NP_001172019.1
Bos taurus (cattle)	GRIN1	XP_002691748.3
Mus musculus (house mouse)	Grin1	NP_032195.1
Rattus norvegicus (Norway rat)	Grin1	NP_001257535.1
Gallus gallus (chicken)	GRIN1	NP_996862.1
	GRIN1	XP_001117773.1
Canis lupus familiaris (dog)	GRIN1	NP_001008717.1
Danio rerio (zebrafish)	grin1a	NP_001070182.2
Drosophila melanogaster (fruit fly)	Nmdar1	NP_730940.1
Arabidopsis thaliana (thale cress)	GLR5	NP_565744.1

Gene Ontology
Bibliography

Related articles in PubMed

Pla2g6 Deficiency in Zebrafish Leads to Dopaminergic Cell Death, Axonal Degeneration, Increased ?-Synuclein Expression, and Defects in Brain Functions and Pathways.
S?nchez E, Azcona LJ, Pais?n-Ruiz C
Molecular neurobiology(2018 Jan)

Effects of 2,2',4,4'-tetrabromodiphenyl ether on neurobehavior and memory change and bcl-2, c-fos, grin1b and lingo1b gene expression in male zebrafish (Danio rerio).
Zheng S, Liu C, Huang Y, Bao M, Huang Y, Wu K
Toxicology and applied pharmacology33310-16(2017 10)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Using a comparative sequence-based approach and sequence analysis, two novel RNA editing sites were located in grin1b transcripts of embryonic Danio rerio.
Title: Identification and characterization of two novel RNA editing sites in grin1b transcripts of embryonic Danio rerio.

The following grin1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the grin1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa00083	NM_001144131.1 Latest version!	Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 1b (grin1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa10404	XM_005171776.2 Latest version!	Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 1b (grin1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa10405	XM_005171777.2 Latest version!	Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 1b (grin1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
View Old Accession Versions >>
ODa10404	XM_005171776.1	Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 1b (grin1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa10405	XM_005171777.1	Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 1b (grin1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

grin1b ( NM_001144131.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001137603.1 Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 1b (grin1b), mRNA.

Price & Availability↑

CloneID	ODa00083
Clone ID Related Accession (Same CDS sequence)	NM_001144131.1
Accession Version	NM_001144131.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2814bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-05
Organism	Danio rerio(zebrafish)
Product	glutamate receptor, ionotropic, N-methyl D-aspartate 1b precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX276128.8 and AL831768.9. On Mar 11, 2009 this sequence version replaced XM_001340391.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCGCCTGG TTCTGTTCGC CTTTCTCATC TCGTGCTCCT GCGCGAGAGG CGGATGCGAA 
CCCAAGACTG TGAACATTGG AGCTGTTCTG AGCCAGAAGA GATATGAACA AGTGTTCAAA 
GACGCCGTCA CCCAAGCCAA CAATATCTAC GGCAAAGATA AGTTCAAGAT GAACGCGATT 
TCAGTCACCC ATAAGCCAAA CGCCATCCAG ATGGCACTCT CCGTGTGCGA GGATCTCATC 
TCTAACCAGG TTTATGCGAT TTTGGTAAGC CACCCTCCCC AGTCCAATGA CCATCTGACA 
CCCACTCCGG TGTCCTACAC GGCTGGCTTT TACCGTATCC CTGTAGTGGG CCTGACCACC 
CGCATGTCCA TCTATTCTGA TAAGAGTATC CACCTCTCCT TCCTGCGCAC GGTGCCTCCC 
TATTCCCACC AAGCCCAAGT GTGGTTTGAC ATGATGCGCG AGTTCCAGTG GAATCACATC 
ATCCTGATTG TGAGCGACGA CCACGAGGGC AGAGCGGCAC AGAAAAGGCT GGAAACCCTT 
CTGGAGGAGA GGGAAACAAA GAGTAAAAAC AGGAACTATG AAAACCTCGA CCAACTGTCC 
TTTGACAACA AGCGAGGACC CAAGGCAGAG AAAGTCCTCC TGTTCAGCCA AGACACAAAT 
CTGACGGCTC TGCTCCAGGA AGCCAAGGAG CTTGAGGCCC GAGTTATCAT CCTGTCTGCA 
AGTGAAGACG AAGCCGCGGC CATTTATAAA GTGGCACGCC AGCTCAATAT GACAGGCTCT 
GGTTACGTGT GGCTGGTTGG AGAAAGGGAG ATGTCTGGTA AAGCACTGAG TGAAGCCCCA 
GATGGCTTGC TCGGCCTCCA GCTAATCAAT GGCAAGAACG AGTCTGCGCA CATCTACGAT 
GCCGTGGCTG TGGTGGCCCA GTCCATCCAG GAGCTCTTTG AGAAGGAGAA TATCACAGAG 
CCTCCTCGAG GCTGTGTTGG CAATACAAAC ATCTGGAAGA CTGGCCCACT CTTCAAACGA 
GTGCTGATGT CATCCAAGTA TCCAGATGGC CTGACAGGCC GCGTGGAGTT TAATGATGAC 
GGAGACAGGA GGTTCGCCCA CTACAGCATT CTGAACTACC AGAAAACCCG GCTGGTGCAA 
GTGGGCGTCT ACAATGGCTC GCAAGTTGTA ATGAACGCTC AGAGGAAGAT TATTTGGCCA 
GGAGGAGAGA CTGAAAAGCC GAAAGGCTAT CAGATGTCAA CTAGATTAAA GATTGTAACA 
ATTCATCAAG AGCCCTTTGT CTACGTGAAG CCGACTTTAC GAGATGGAAC CTGTAAGGAA 
GAGCACACTG TAAATGGAGT CAAAATCAAA AAAGTGATCT GCACCGGCCC AAACGAGACC 
ATTCCAGGTC GCCCCATAGT GCCTCAGTGC TGCTATGGGT TCTGCATTGA CCTTTTGATC 
AAACTTGCAT TCACAATGAA CTTCACCTAT GAAGTGCATC TTGTGGCTGA TGGCAAATTT 
GGCACTCAGG AGCGTGTAAA TAACAGCAAC AAGAAAGAGT GGAATGGCAT GATGGGAGAG 
CTTCTGAGTG GCCTGGCAGA CATGATCGTG GCTCCACTTA CAATCAACAA TGAACGAGCC 
CAGTACATAG AGTTCTCTAA ACCGTTCAAA TACCAGGGGC TCACAATACT TGTCAAAAAG 
GAAATACCAC GCAGTACACT GGACTCGTTC ATGCAGCCCT TTCAGAGCAC TCTGTGGTTA 
CTGGTGGGTC TATCGGTCCA TGTTGTGGCG GTGATGCTCT ACCTTTTAGA CCGTTTCAGC 
CCATTTGGAA GGTTTAAAGT GAATAGTGAA GAGGAAGAAG AAGACGCCCT CACCTTATCC 
TCCGCTATGT GGTTCTCCTG GGGTGTACTT CTGAACTCCG GCATTGGAGA AGGTGCCCCT 
CGGAGCTTTT CGGCGAGGAT TTTAGGTATG GTGTGGGCTG GTTTTGCTAT GATTATAGTT 
GCATCTTATA CTGCCAATTT GGCAGCCTTC CTAGTGCTGG ATCGGCCTGA GGAGCGCATT 
ACCGGCATCA ACGACCCTAG GCTGCGAAAC CCATCAGATA AGTTCATCTA CGCCACAGTG 
AAGCAGAGCT CTGTGGACAT TTATTTCCGG CGCCAAGTTG AGCTTAGCAC CATGTACCGC 
CACATGGAAA AGCACAACTA CGAGAGCGCC GCTGAAGCCA TCCAGGCTGT GCGAGACAAC 
AAACTCCACG CATTTATATG GGATTCAGCA GTGTTGGAGT TTGAGGCCTC GCAGAAATGT 
GACCTGGTTA CTACGGGCGA GCTGTTCTTC CGCTCTGGAT TCGGTATCGG CATGCGTAAA 
GACAGCCCTT GGAAGCAAAA CGTCTCTCTG GCCATCCTCA GTTCCCATGA GAACGGCTTC 
ATGGAGGACT TGGATAAAAC CTGGGTTCGA TATCAGGAGT GTGACTCCAG AAGCAATGCC 
CCAGCCACGC TCACTTTTGA GAATATGGCA GGCGTGTTCA TGTTGGTGGC TGGTGGCATA 
GTCGCCGGGA TCTTCCTCAT CTTCATTGAG ATTGCATACA AACGACACAA GGACGCCCGC 
AGGAAGCAAA TGCAGCTGGC CTTTGCAGCA GTTAACGTCT GGAGGAAGAA CCTACAGGAT 
AGGAAAAGTG GTAGAGCAGA GCCCGACCCC AAAAAGAAAG CCTCTTTTAG GTCCATCAGT 
ACCAACCTGG CCTCCAACAT CAAGAGACGT AGGTCGTCCA AAGACACGCC ATATCCCACG 
GACATTACGG GCCAGCTCAA CCTGTCAGAC CCCTCTGTGA GCACAGTGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137603.1
CDS	601..3414
Translation	MRLVLFAFLISCSCARGGCEPKTVNIGAVLSQKRYEQVFKDAVTQANNIYGKDKFKMNAISVTHKPNAIQMALSVCEDLISNQVYAILVSHPPQSNDHLTPTPVSYTAGFYRIPVVGLTTRMSIYSDKSIHLSFLRTVPPYSHQAQVWFDMMREFQWNHIILIVSDDHEGRAAQKRLETLLEERETKSKNRNYENLDQLSFDNKRGPKAEKVLLFSQDTNLTALLQEAKELEARVIILSASEDEAAAIYKVARQLNMTGSGYVWLVGEREMSGKALSEAPDGLLGLQLINGKNESAHIYDAVAVVAQSIQELFEKENITEPPRGCVGNTNIWKTGPLFKRVLMSSKYPDGLTGRVEFNDDGDRRFAHYSILNYQKTRLVQVGVYNGSQVVMNAQRKIIWPGGETEKPKGYQMSTRLKIVTIHQEPFVYVKPTLRDGTCKEEHTVNGVKIKKVICTGPNETIPGRPIVPQCCYGFCIDLLIKLAFTMNFTYEVHLVADGKFGTQERVNNSNKKEWNGMMGELLSGLADMIVAPLTINNERAQYIEFSKPFKYQGLTILVKKEIPRSTLDSFMQPFQSTLWLLVGLSVHVVAVMLYLLDRFSPFGRFKVNSEEEEEDALTLSSAMWFSWGVLLNSGIGEGAPRSFSARILGMVWAGFAMIIVASYTANLAAFLVLDRPEERITGINDPRLRNPSDKFIYATVKQSSVDIYFRRQVELSTMYRHMEKHNYESAAEAIQAVRDNKLHAFIWDSAVLEFEASQKCDLVTTGELFFRSGFGIGMRKDSPWKQNVSLAILSSHENGFMEDLDKTWVRYQECDSRSNAPATLTFENMAGVFMLVAGGIVAGIFLIFIEIAYKRHKDARRKQMQLAFAAVNVWRKNLQDRKSGRAEPDPKKKASFRSISTNLASNIKRRRSSKDTPYPTDITGQLNLSDPSVSTVV

Target ORF information:

RefSeq Version	NM_001144131.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 1b (grin1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144131.1

ORF Insert Sequence:

ATGCGCCTGG TTCTGTTCGC CTTTCTCATC TCGTGCTCCT GCGCGAGAGG CGGATGCGAA 
CCCAAGACTG TGAACATTGG AGCTGTTCTG AGCCAGAAGA GATATGAACA AGTGTTCAAA 
GACGCCGTCA CCCAAGCCAA CAATATCTAC GGCAAAGATA AGTTCAAGAT GAACGCGATT 
TCAGTCACCC ATAAGCCAAA CGCCATCCAG ATGGCACTCT CCGTGTGCGA GGATCTCATC 
TCTAACCAGG TTTATGCGAT TTTGGTAAGC CACCCTCCCC AGTCCAATGA CCATCTGACA 
CCCACTCCGG TGTCCTACAC GGCTGGCTTT TACCGTATCC CTGTAGTGGG CCTGACCACC 
CGCATGTCCA TCTATTCTGA TAAGAGTATC CACCTCTCCT TCCTGCGCAC GGTGCCTCCC 
TATTCCCACC AAGCCCAAGT GTGGTTTGAC ATGATGCGCG AGTTCCAGTG GAATCACATC 
ATCCTGATTG TGAGCGACGA CCACGAGGGC AGAGCGGCAC AGAAAAGGCT GGAAACCCTT 
CTGGAGGAGA GGGAAACAAA GAGTAAAAAC AGGAACTATG AAAACCTCGA CCAACTGTCC 
TTTGACAACA AGCGAGGACC CAAGGCAGAG AAAGTCCTCC TGTTCAGCCA AGACACAAAT 
CTGACGGCTC TGCTCCAGGA AGCCAAGGAG CTTGAGGCCC GAGTTATCAT CCTGTCTGCA 
AGTGAAGACG AAGCCGCGGC CATTTATAAA GTGGCACGCC AGCTCAATAT GACAGGCTCT 
GGTTACGTGT GGCTGGTTGG AGAAAGGGAG ATGTCTGGTA AAGCACTGAG TGAAGCCCCA 
GATGGCTTGC TCGGCCTCCA GCTAATCAAT GGCAAGAACG AGTCTGCGCA CATCTACGAT 
GCCGTGGCTG TGGTGGCCCA GTCCATCCAG GAGCTCTTTG AGAAGGAGAA TATCACAGAG 
CCTCCTCGAG GCTGTGTTGG CAATACAAAC ATCTGGAAGA CTGGCCCACT CTTCAAACGA 
GTGCTGATGT CATCCAAGTA TCCAGATGGC CTGACAGGCC GCGTGGAGTT TAATGATGAC 
GGAGACAGGA GGTTCGCCCA CTACAGCATT CTGAACTACC AGAAAACCCG GCTGGTGCAA 
GTGGGCGTCT ACAATGGCTC GCAAGTTGTA ATGAACGCTC AGAGGAAGAT TATTTGGCCA 
GGAGGAGAGA CTGAAAAGCC GAAAGGCTAT CAGATGTCAA CTAGATTAAA GATTGTAACA 
ATTCATCAAG AGCCCTTTGT CTACGTGAAG CCGACTTTAC GAGATGGAAC CTGTAAGGAA 
GAGCACACTG TAAATGGAGT CAAAATCAAA AAAGTGATCT GCACCGGCCC AAACGAGACC 
ATTCCAGGTC GCCCCATAGT GCCTCAGTGC TGCTATGGGT TCTGCATTGA CCTTTTGATC 
AAACTTGCAT TCACAATGAA CTTCACCTAT GAAGTGCATC TTGTGGCTGA TGGCAAATTT 
GGCACTCAGG AGCGTGTAAA TAACAGCAAC AAGAAAGAGT GGAATGGCAT GATGGGAGAG 
CTTCTGAGTG GCCTGGCAGA CATGATCGTG GCTCCACTTA CAATCAACAA TGAACGAGCC 
CAGTACATAG AGTTCTCTAA ACCGTTCAAA TACCAGGGGC TCACAATACT TGTCAAAAAG 
GAAATACCAC GCAGTACACT GGACTCGTTC ATGCAGCCCT TTCAGAGCAC TCTGTGGTTA 
CTGGTGGGTC TATCGGTCCA TGTTGTGGCG GTGATGCTCT ACCTTTTAGA CCGTTTCAGC 
CCATTTGGAA GGTTTAAAGT GAATAGTGAA GAGGAAGAAG AAGACGCCCT CACCTTATCC 
TCCGCTATGT GGTTCTCCTG GGGTGTACTT CTGAACTCCG GCATTGGAGA AGGTGCCCCT 
CGGAGCTTTT CGGCGAGGAT TTTAGGTATG GTGTGGGCTG GTTTTGCTAT GATTATAGTT 
GCATCTTATA CTGCCAATTT GGCAGCCTTC CTAGTGCTGG ATCGGCCTGA GGAGCGCATT 
ACCGGCATCA ACGACCCTAG GCTGCGAAAC CCATCAGATA AGTTCATCTA CGCCACAGTG 
AAGCAGAGCT CTGTGGACAT TTATTTCCGG CGCCAAGTTG AGCTTAGCAC CATGTACCGC 
CACATGGAAA AGCACAACTA CGAGAGCGCC GCTGAAGCCA TCCAGGCTGT GCGAGACAAC 
AAACTCCACG CATTTATATG GGATTCAGCA GTGTTGGAGT TTGAGGCCTC GCAGAAATGT 
GACCTGGTTA CTACGGGCGA GCTGTTCTTC CGCTCTGGAT TCGGTATCGG CATGCGTAAA 
GACAGCCCTT GGAAGCAAAA CGTCTCTCTG GCCATCCTCA GTTCCCATGA GAACGGCTTC 
ATGGAGGACT TGGATAAAAC CTGGGTTCGA TATCAGGAGT GTGACTCCAG AAGCAATGCC 
CCAGCCACGC TCACTTTTGA GAATATGGCA GGCGTGTTCA TGTTGGTGGC TGGTGGCATA 
GTCGCCGGGA TCTTCCTCAT CTTCATTGAG ATTGCATACA AACGACACAA GGACGCCCGC 
AGGAAGCAAA TGCAGCTGGC CTTTGCAGCA GTTAACGTCT GGAGGAAGAA CCTACAGGAT 
AGGAAAAGTG GTAGAGCAGA GCCCGACCCC AAAAAGAAAG CCTCTTTTAG GTCCATCAGT 
ACCAACCTGG CCTCCAACAT CAAGAGACGT AGGTCGTCCA AAGACACGCC ATATCCCACG 
GACATTACGG GCCAGCTCAA CCTGTCAGAC CCCTCTGTGA GCACAGTGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

grin1b ( XM_005171776.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171833.1 Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 1b (grin1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa10404
Clone ID Related Accession (Same CDS sequence)	XM_005171776.2 , XM_005171776.1
Accession Version	XM_005171776.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	glutamate receptor, ionotropic, N-methyl D-aspartate 1b isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005171776.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCCTGG TTCTGTTCGC CTTTCTCATC TCGTGCTCCT GCGCGAGAGG CGGATGCGAA 
CCCAAGACTG TGAACATTGG AGCTGTTCTG AGCCAGAAGA GATATGAACA AGTGTTCAAA 
GACGCCGTCA CCCAAGCCAA CAATATCTAC GGCAAAGATA AGTTCAAGAT GAACGCGATT 
TCAGTCACCC ATAAGCCAAA CGCCATCCAG ATGGCACTCT CCGTGTGCGA GGATCTCATC 
TCTAACCAGG TTTATGCGAT TTTGGTAAGC CACCCTCCCC AGTCCAATGA CCATCTGACA 
CCCACTCCGG TGTCCTACAC GGCTGGCTTT TACCGTATCC CTGTAGTGGG CCTGACCACC 
CGCATGTCCA TCTATTCTGA TAAGAGTATC CACCTCTCCT TCCTGCGCAC GGTGCCTCCC 
TATTCCCACC AAGCCCAAGT GTGGTTTGAC ATGATGCGCG AGTTCCAGTG GAATCACATC 
ATCCTGATTG TGAGCGACGA CCACGAGGGC AGAGCGGCAC AGAAAAGGCT GGAAACCCTT 
CTGGAGGAGA GGGAAACAAA GGCAGAGAAA GTCCTCCTGT TCAGCCAAGA CACAAATCTG 
ACGGCTCTGC TCCAGGAAGC CAAGGAGCTT GAGGCCCGAG TTATCATCCT GTCTGCAAGT 
GAAGACGAAG CCGCGGCCAT TTATAAAGTG GCACGCCAGC TCAATATGAC AGGCTCTGGT 
TACGTGTGGC TGGTTGGAGA AAGGGAGATG TCTGGTAAAG CACTGAGTGA AGCCCCAGAT 
GGCTTGCTCG GCCTCCAGCT AATCAATGGC AAGAACGAGT CTGCGCACAT CTACGATGCC 
GTGGCTGTGG TGGCCCAGTC CATCCAGGAG CTCTTTGAGA AGGAGAATAT CACAGAGCCT 
CCTCGAGGCT GTGTTGGCAA TACAAACATC TGGAAGACTG GCCCACTCTT CAAACGAGTG 
CTGATGTCAT CCAAGTATCC AGATGGCCTG ACAGGCCGCG TGGAGTTTAA TGATGACGGA 
GACAGGAGGT TCGCCCACTA CAGCATTCTG AACTACCAGA AAACCCGGCT GGTGCAAGTG 
GGCGTCTACA ATGGCTCGCA AGTTGTAATG AACGCTCAGA GGAAGATTAT TTGGCCAGGA 
GGAGAGACTG AAAAGCCGAA AGGCTATCAG ATGTCAACTA GATTAAAGAT TGTAACAATT 
CATCAAGAGC CCTTTGTCTA CGTGAAGCCG ACTTTACGAG ATGGAACCTG TAAGGAAGAG 
CACACTGTAA ATGGAGTCAA AATCAAAAAA GTGATCTGCA CCGGCCCAAA CGAGACCATT 
CCAGGTCGCC CCATAGTGCC TCAGTGCTGC TATGGGTTCT GCATTGACCT TTTGATCAAA 
CTTGCATTCA CAATGAACTT CACCTATGAA GTGCATCTTG TGGCTGATGG CAAATTTGGC 
ACTCAGGAGC GTGTAAATAA CAGCAACAAG AAAGAGTGGA ATGGCATGAT GGGAGAGCTT 
CTGAGTGGCC TGGCAGACAT GATCGTGGCT CCACTTACAA TCAACAATGA ACGAGCCCAG 
TACATAGAGT TCTCTAAACC GTTCAAATAC CAGGGGCTCA CAATACTTGT CAAAAAGGAA 
ATACCACGCA GTACACTGGA CTCGTTCATG CAGCCCTTTC AGAGCACTCT GTGGTTACTG 
GTGGGTCTAT CGGTCCATGT TGTGGCGGTG ATGCTCTACC TTTTAGACCG TTTCAGCCCA 
TTTGGAAGGT TTAAAGTGAA TAGTGAAGAG GAAGAAGAAG ACGCCCTCAC CTTATCCTCC 
GCTATGTGGT TCTCCTGGGG TGTACTTCTG AACTCCGGCA TTGGAGAAGG TGCCCCTCGG 
AGCTTTTCGG CGAGGATTTT AGGTATGGTG TGGGCTGGTT TTGCTATGAT TATAGTTGCA 
TCTTATACTG CCAATTTGGC AGCCTTCCTA GTGCTGGATC GGCCTGAGGA GCGCATTACC 
GGCATCAACG ACCCTAGGCT GCGAAACCCA TCAGATAAGT TCATCTACGC CACAGTGAAG 
CAGAGCTCTG TGGACATTTA TTTCCGGCGC CAAGTTGAGC TTAGCACCAT GTACCGCCAC 
ATGGAAAAGC ACAACTACGA GAGCGCCGCT GAAGCCATCC AGGCTGTGCG AGACAACAAA 
CTCCACGCAT TTATATGGGA TTCAGCAGTG TTGGAGTTTG AGGCCTCGCA GAAATGTGAC 
CTGGTTACTA CGGGCGAGCT GTTCTTCCGC TCTGGATTCG GTATCGGCAT GCGTAAAGAC 
AGCCCTTGGA AGCAAAACGT CTCTCTGGCC ATCCTCAGTT CCCATGAGAA CGGCTTCATG 
GAGGACTTGG ATAAAACCTG GGTTCGATAT CAGGAGTGTG ACTCCAGAAG CAATGCCCCA 
GCCACGCTCA CTTTTGAGAA TATGGCAGGC GTGTTCATGT TGGTGGCTGG TGGCATAGTC 
GCCGGGATCT TCCTCATCTT CATTGAGATT GCATACAAAC GACACAAGGA CGCCCGCAGG 
AAGCAAATGC AGCTGGCCTT TGCAGCAGTT AACGTCTGGA GGAAGAACCT ACAGGATAGG 
AAAAGTGGTA GAGCAGAGCC CGACCCCAAA AAGAAAGCCT CTTTTAGGTC CATCAGTACC 
AACCTGGCCT CCAACATCAA GAGACGTAGG TCGTCCAAAG ACACGCCATA TCCCACGGAC 
ATTACGGGCC AGCTCAACCT GTCAGACCCC TCTGTGAGCA CAGTGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171833.1
CDS	1..2751
Translation	MRLVLFAFLISCSCARGGCEPKTVNIGAVLSQKRYEQVFKDAVTQANNIYGKDKFKMNAISVTHKPNAIQMALSVCEDLISNQVYAILVSHPPQSNDHLTPTPVSYTAGFYRIPVVGLTTRMSIYSDKSIHLSFLRTVPPYSHQAQVWFDMMREFQWNHIILIVSDDHEGRAAQKRLETLLEERETKAEKVLLFSQDTNLTALLQEAKELEARVIILSASEDEAAAIYKVARQLNMTGSGYVWLVGEREMSGKALSEAPDGLLGLQLINGKNESAHIYDAVAVVAQSIQELFEKENITEPPRGCVGNTNIWKTGPLFKRVLMSSKYPDGLTGRVEFNDDGDRRFAHYSILNYQKTRLVQVGVYNGSQVVMNAQRKIIWPGGETEKPKGYQMSTRLKIVTIHQEPFVYVKPTLRDGTCKEEHTVNGVKIKKVICTGPNETIPGRPIVPQCCYGFCIDLLIKLAFTMNFTYEVHLVADGKFGTQERVNNSNKKEWNGMMGELLSGLADMIVAPLTINNERAQYIEFSKPFKYQGLTILVKKEIPRSTLDSFMQPFQSTLWLLVGLSVHVVAVMLYLLDRFSPFGRFKVNSEEEEEDALTLSSAMWFSWGVLLNSGIGEGAPRSFSARILGMVWAGFAMIIVASYTANLAAFLVLDRPEERITGINDPRLRNPSDKFIYATVKQSSVDIYFRRQVELSTMYRHMEKHNYESAAEAIQAVRDNKLHAFIWDSAVLEFEASQKCDLVTTGELFFRSGFGIGMRKDSPWKQNVSLAILSSHENGFMEDLDKTWVRYQECDSRSNAPATLTFENMAGVFMLVAGGIVAGIFLIFIEIAYKRHKDARRKQMQLAFAAVNVWRKNLQDRKSGRAEPDPKKKASFRSISTNLASNIKRRRSSKDTPYPTDITGQLNLSDPSVSTVV

Target ORF information:

RefSeq Version	XM_005171776.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 1b (grin1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171776.2

ORF Insert Sequence:

ATGCGCCTGG TTCTGTTCGC CTTTCTCATC TCGTGCTCCT GCGCGAGAGG CGGATGCGAA 
CCCAAGACTG TGAACATTGG AGCTGTTCTG AGCCAGAAGA GATATGAACA AGTGTTCAAA 
GACGCCGTCA CCCAAGCCAA CAATATCTAC GGCAAAGATA AGTTCAAGAT GAACGCGATT 
TCAGTCACCC ATAAGCCAAA CGCCATCCAG ATGGCACTCT CCGTGTGCGA GGATCTCATC 
TCTAACCAGG TTTATGCGAT TTTGGTAAGC CACCCTCCCC AGTCCAATGA CCATCTGACA 
CCCACTCCGG TGTCCTACAC GGCTGGCTTT TACCGTATCC CTGTAGTGGG CCTGACCACC 
CGCATGTCCA TCTATTCTGA TAAGAGTATC CACCTCTCCT TCCTGCGCAC GGTGCCTCCC 
TATTCCCACC AAGCCCAAGT GTGGTTTGAC ATGATGCGCG AGTTCCAGTG GAATCACATC 
ATCCTGATTG TGAGCGACGA CCACGAGGGC AGAGCGGCAC AGAAAAGGCT GGAAACCCTT 
CTGGAGGAGA GGGAAACAAA GGCAGAGAAA GTCCTCCTGT TCAGCCAAGA CACAAATCTG 
ACGGCTCTGC TCCAGGAAGC CAAGGAGCTT GAGGCCCGAG TTATCATCCT GTCTGCAAGT 
GAAGACGAAG CCGCGGCCAT TTATAAAGTG GCACGCCAGC TCAATATGAC AGGCTCTGGT 
TACGTGTGGC TGGTTGGAGA AAGGGAGATG TCTGGTAAAG CACTGAGTGA AGCCCCAGAT 
GGCTTGCTCG GCCTCCAGCT AATCAATGGC AAGAACGAGT CTGCGCACAT CTACGATGCC 
GTGGCTGTGG TGGCCCAGTC CATCCAGGAG CTCTTTGAGA AGGAGAATAT CACAGAGCCT 
CCTCGAGGCT GTGTTGGCAA TACAAACATC TGGAAGACTG GCCCACTCTT CAAACGAGTG 
CTGATGTCAT CCAAGTATCC AGATGGCCTG ACAGGCCGCG TGGAGTTTAA TGATGACGGA 
GACAGGAGGT TCGCCCACTA CAGCATTCTG AACTACCAGA AAACCCGGCT GGTGCAAGTG 
GGCGTCTACA ATGGCTCGCA AGTTGTAATG AACGCTCAGA GGAAGATTAT TTGGCCAGGA 
GGAGAGACTG AAAAGCCGAA AGGCTATCAG ATGTCAACTA GATTAAAGAT TGTAACAATT 
CATCAAGAGC CCTTTGTCTA CGTGAAGCCG ACTTTACGAG ATGGAACCTG TAAGGAAGAG 
CACACTGTAA ATGGAGTCAA AATCAAAAAA GTGATCTGCA CCGGCCCAAA CGAGACCATT 
CCAGGTCGCC CCATAGTGCC TCAGTGCTGC TATGGGTTCT GCATTGACCT TTTGATCAAA 
CTTGCATTCA CAATGAACTT CACCTATGAA GTGCATCTTG TGGCTGATGG CAAATTTGGC 
ACTCAGGAGC GTGTAAATAA CAGCAACAAG AAAGAGTGGA ATGGCATGAT GGGAGAGCTT 
CTGAGTGGCC TGGCAGACAT GATCGTGGCT CCACTTACAA TCAACAATGA ACGAGCCCAG 
TACATAGAGT TCTCTAAACC GTTCAAATAC CAGGGGCTCA CAATACTTGT CAAAAAGGAA 
ATACCACGCA GTACACTGGA CTCGTTCATG CAGCCCTTTC AGAGCACTCT GTGGTTACTG 
GTGGGTCTAT CGGTCCATGT TGTGGCGGTG ATGCTCTACC TTTTAGACCG TTTCAGCCCA 
TTTGGAAGGT TTAAAGTGAA TAGTGAAGAG GAAGAAGAAG ACGCCCTCAC CTTATCCTCC 
GCTATGTGGT TCTCCTGGGG TGTACTTCTG AACTCCGGCA TTGGAGAAGG TGCCCCTCGG 
AGCTTTTCGG CGAGGATTTT AGGTATGGTG TGGGCTGGTT TTGCTATGAT TATAGTTGCA 
TCTTATACTG CCAATTTGGC AGCCTTCCTA GTGCTGGATC GGCCTGAGGA GCGCATTACC 
GGCATCAACG ACCCTAGGCT GCGAAACCCA TCAGATAAGT TCATCTACGC CACAGTGAAG 
CAGAGCTCTG TGGACATTTA TTTCCGGCGC CAAGTTGAGC TTAGCACCAT GTACCGCCAC 
ATGGAAAAGC ACAACTACGA GAGCGCCGCT GAAGCCATCC AGGCTGTGCG AGACAACAAA 
CTCCACGCAT TTATATGGGA TTCAGCAGTG TTGGAGTTTG AGGCCTCGCA GAAATGTGAC 
CTGGTTACTA CGGGCGAGCT GTTCTTCCGC TCTGGATTCG GTATCGGCAT GCGTAAAGAC 
AGCCCTTGGA AGCAAAACGT CTCTCTGGCC ATCCTCAGTT CCCATGAGAA CGGCTTCATG 
GAGGACTTGG ATAAAACCTG GGTTCGATAT CAGGAGTGTG ACTCCAGAAG CAATGCCCCA 
GCCACGCTCA CTTTTGAGAA TATGGCAGGC GTGTTCATGT TGGTGGCTGG TGGCATAGTC 
GCCGGGATCT TCCTCATCTT CATTGAGATT GCATACAAAC GACACAAGGA CGCCCGCAGG 
AAGCAAATGC AGCTGGCCTT TGCAGCAGTT AACGTCTGGA GGAAGAACCT ACAGGATAGG 
AAAAGTGGTA GAGCAGAGCC CGACCCCAAA AAGAAAGCCT CTTTTAGGTC CATCAGTACC 
AACCTGGCCT CCAACATCAA GAGACGTAGG TCGTCCAAAG ACACGCCATA TCCCACGGAC 
ATTACGGGCC AGCTCAACCT GTCAGACCCC TCTGTGAGCA CAGTGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

grin1b ( XM_005171776.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171833.1 Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 1b (grin1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa10404
Clone ID Related Accession (Same CDS sequence)	XM_005171776.2 , XM_005171776.1
Accession Version	XM_005171776.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	glutamate receptor, ionotropic, N-methyl D-aspartate 1b isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCCTGG TTCTGTTCGC CTTTCTCATC TCGTGCTCCT GCGCGAGAGG CGGATGCGAA 
CCCAAGACTG TGAACATTGG AGCTGTTCTG AGCCAGAAGA GATATGAACA AGTGTTCAAA 
GACGCCGTCA CCCAAGCCAA CAATATCTAC GGCAAAGATA AGTTCAAGAT GAACGCGATT 
TCAGTCACCC ATAAGCCAAA CGCCATCCAG ATGGCACTCT CCGTGTGCGA GGATCTCATC 
TCTAACCAGG TTTATGCGAT TTTGGTAAGC CACCCTCCCC AGTCCAATGA CCATCTGACA 
CCCACTCCGG TGTCCTACAC GGCTGGCTTT TACCGTATCC CTGTAGTGGG CCTGACCACC 
CGCATGTCCA TCTATTCTGA TAAGAGTATC CACCTCTCCT TCCTGCGCAC GGTGCCTCCC 
TATTCCCACC AAGCCCAAGT GTGGTTTGAC ATGATGCGCG AGTTCCAGTG GAATCACATC 
ATCCTGATTG TGAGCGACGA CCACGAGGGC AGAGCGGCAC AGAAAAGGCT GGAAACCCTT 
CTGGAGGAGA GGGAAACAAA GGCAGAGAAA GTCCTCCTGT TCAGCCAAGA CACAAATCTG 
ACGGCTCTGC TCCAGGAAGC CAAGGAGCTT GAGGCCCGAG TTATCATCCT GTCTGCAAGT 
GAAGACGAAG CCGCGGCCAT TTATAAAGTG GCACGCCAGC TCAATATGAC AGGCTCTGGT 
TACGTGTGGC TGGTTGGAGA AAGGGAGATG TCTGGTAAAG CACTGAGTGA AGCCCCAGAT 
GGCTTGCTCG GCCTCCAGCT AATCAATGGC AAGAACGAGT CTGCGCACAT CTACGATGCC 
GTGGCTGTGG TGGCCCAGTC CATCCAGGAG CTCTTTGAGA AGGAGAATAT CACAGAGCCT 
CCTCGAGGCT GTGTTGGCAA TACAAACATC TGGAAGACTG GCCCACTCTT CAAACGAGTG 
CTGATGTCAT CCAAGTATCC AGATGGCCTG ACAGGCCGCG TGGAGTTTAA TGATGACGGA 
GACAGGAGGT TCGCCCACTA CAGCATTCTG AACTACCAGA AAACCCGGCT GGTGCAAGTG 
GGCGTCTACA ATGGCTCGCA AGTTGTAATG AACGCTCAGA GGAAGATTAT TTGGCCAGGA 
GGAGAGACTG AAAAGCCGAA AGGCTATCAG ATGTCAACTA GATTAAAGAT TGTAACAATT 
CATCAAGAGC CCTTTGTCTA CGTGAAGCCG ACTTTACGAG ATGGAACCTG TAAGGAAGAG 
CACACTGTAA ATGGAGTCAA AATCAAAAAA GTGATCTGCA CCGGCCCAAA CGAGACCATT 
CCAGGTCGCC CCATAGTGCC TCAGTGCTGC TATGGGTTCT GCATTGACCT TTTGATCAAA 
CTTGCATTCA CAATGAACTT CACCTATGAA GTGCATCTTG TGGCTGATGG CAAATTTGGC 
ACTCAGGAGC GTGTAAATAA CAGCAACAAG AAAGAGTGGA ATGGCATGAT GGGAGAGCTT 
CTGAGTGGCC TGGCAGACAT GATCGTGGCT CCACTTACAA TCAACAATGA ACGAGCCCAG 
TACATAGAGT TCTCTAAACC GTTCAAATAC CAGGGGCTCA CAATACTTGT CAAAAAGGAA 
ATACCACGCA GTACACTGGA CTCGTTCATG CAGCCCTTTC AGAGCACTCT GTGGTTACTG 
GTGGGTCTAT CGGTCCATGT TGTGGCGGTG ATGCTCTACC TTTTAGACCG TTTCAGCCCA 
TTTGGAAGGT TTAAAGTGAA TAGTGAAGAG GAAGAAGAAG ACGCCCTCAC CTTATCCTCC 
GCTATGTGGT TCTCCTGGGG TGTACTTCTG AACTCCGGCA TTGGAGAAGG TGCCCCTCGG 
AGCTTTTCGG CGAGGATTTT AGGTATGGTG TGGGCTGGTT TTGCTATGAT TATAGTTGCA 
TCTTATACTG CCAATTTGGC AGCCTTCCTA GTGCTGGATC GGCCTGAGGA GCGCATTACC 
GGCATCAACG ACCCTAGGCT GCGAAACCCA TCAGATAAGT TCATCTACGC CACAGTGAAG 
CAGAGCTCTG TGGACATTTA TTTCCGGCGC CAAGTTGAGC TTAGCACCAT GTACCGCCAC 
ATGGAAAAGC ACAACTACGA GAGCGCCGCT GAAGCCATCC AGGCTGTGCG AGACAACAAA 
CTCCACGCAT TTATATGGGA TTCAGCAGTG TTGGAGTTTG AGGCCTCGCA GAAATGTGAC 
CTGGTTACTA CGGGCGAGCT GTTCTTCCGC TCTGGATTCG GTATCGGCAT GCGTAAAGAC 
AGCCCTTGGA AGCAAAACGT CTCTCTGGCC ATCCTCAGTT CCCATGAGAA CGGCTTCATG 
GAGGACTTGG ATAAAACCTG GGTTCGATAT CAGGAGTGTG ACTCCAGAAG CAATGCCCCA 
GCCACGCTCA CTTTTGAGAA TATGGCAGGC GTGTTCATGT TGGTGGCTGG TGGCATAGTC 
GCCGGGATCT TCCTCATCTT CATTGAGATT GCATACAAAC GACACAAGGA CGCCCGCAGG 
AAGCAAATGC AGCTGGCCTT TGCAGCAGTT AACGTCTGGA GGAAGAACCT ACAGGATAGG 
AAAAGTGGTA GAGCAGAGCC CGACCCCAAA AAGAAAGCCT CTTTTAGGTC CATCAGTACC 
AACCTGGCCT CCAACATCAA GAGACGTAGG TCGTCCAAAG ACACGCCATA TCCCACGGAC 
ATTACGGGCC AGCTCAACCT GTCAGACCCC TCTGTGAGCA CAGTGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171833.1
CDS	601..3351
Translation	MRLVLFAFLISCSCARGGCEPKTVNIGAVLSQKRYEQVFKDAVTQANNIYGKDKFKMNAISVTHKPNAIQMALSVCEDLISNQVYAILVSHPPQSNDHLTPTPVSYTAGFYRIPVVGLTTRMSIYSDKSIHLSFLRTVPPYSHQAQVWFDMMREFQWNHIILIVSDDHEGRAAQKRLETLLEERETKAEKVLLFSQDTNLTALLQEAKELEARVIILSASEDEAAAIYKVARQLNMTGSGYVWLVGEREMSGKALSEAPDGLLGLQLINGKNESAHIYDAVAVVAQSIQELFEKENITEPPRGCVGNTNIWKTGPLFKRVLMSSKYPDGLTGRVEFNDDGDRRFAHYSILNYQKTRLVQVGVYNGSQVVMNAQRKIIWPGGETEKPKGYQMSTRLKIVTIHQEPFVYVKPTLRDGTCKEEHTVNGVKIKKVICTGPNETIPGRPIVPQCCYGFCIDLLIKLAFTMNFTYEVHLVADGKFGTQERVNNSNKKEWNGMMGELLSGLADMIVAPLTINNERAQYIEFSKPFKYQGLTILVKKEIPRSTLDSFMQPFQSTLWLLVGLSVHVVAVMLYLLDRFSPFGRFKVNSEEEEEDALTLSSAMWFSWGVLLNSGIGEGAPRSFSARILGMVWAGFAMIIVASYTANLAAFLVLDRPEERITGINDPRLRNPSDKFIYATVKQSSVDIYFRRQVELSTMYRHMEKHNYESAAEAIQAVRDNKLHAFIWDSAVLEFEASQKCDLVTTGELFFRSGFGIGMRKDSPWKQNVSLAILSSHENGFMEDLDKTWVRYQECDSRSNAPATLTFENMAGVFMLVAGGIVAGIFLIFIEIAYKRHKDARRKQMQLAFAAVNVWRKNLQDRKSGRAEPDPKKKASFRSISTNLASNIKRRRSSKDTPYPTDITGQLNLSDPSVSTVV

Target ORF information:

RefSeq Version	XM_005171776.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 1b (grin1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171776.1

ORF Insert Sequence:

ATGCGCCTGG TTCTGTTCGC CTTTCTCATC TCGTGCTCCT GCGCGAGAGG CGGATGCGAA 
CCCAAGACTG TGAACATTGG AGCTGTTCTG AGCCAGAAGA GATATGAACA AGTGTTCAAA 
GACGCCGTCA CCCAAGCCAA CAATATCTAC GGCAAAGATA AGTTCAAGAT GAACGCGATT 
TCAGTCACCC ATAAGCCAAA CGCCATCCAG ATGGCACTCT CCGTGTGCGA GGATCTCATC 
TCTAACCAGG TTTATGCGAT TTTGGTAAGC CACCCTCCCC AGTCCAATGA CCATCTGACA 
CCCACTCCGG TGTCCTACAC GGCTGGCTTT TACCGTATCC CTGTAGTGGG CCTGACCACC 
CGCATGTCCA TCTATTCTGA TAAGAGTATC CACCTCTCCT TCCTGCGCAC GGTGCCTCCC 
TATTCCCACC AAGCCCAAGT GTGGTTTGAC ATGATGCGCG AGTTCCAGTG GAATCACATC 
ATCCTGATTG TGAGCGACGA CCACGAGGGC AGAGCGGCAC AGAAAAGGCT GGAAACCCTT 
CTGGAGGAGA GGGAAACAAA GGCAGAGAAA GTCCTCCTGT TCAGCCAAGA CACAAATCTG 
ACGGCTCTGC TCCAGGAAGC CAAGGAGCTT GAGGCCCGAG TTATCATCCT GTCTGCAAGT 
GAAGACGAAG CCGCGGCCAT TTATAAAGTG GCACGCCAGC TCAATATGAC AGGCTCTGGT 
TACGTGTGGC TGGTTGGAGA AAGGGAGATG TCTGGTAAAG CACTGAGTGA AGCCCCAGAT 
GGCTTGCTCG GCCTCCAGCT AATCAATGGC AAGAACGAGT CTGCGCACAT CTACGATGCC 
GTGGCTGTGG TGGCCCAGTC CATCCAGGAG CTCTTTGAGA AGGAGAATAT CACAGAGCCT 
CCTCGAGGCT GTGTTGGCAA TACAAACATC TGGAAGACTG GCCCACTCTT CAAACGAGTG 
CTGATGTCAT CCAAGTATCC AGATGGCCTG ACAGGCCGCG TGGAGTTTAA TGATGACGGA 
GACAGGAGGT TCGCCCACTA CAGCATTCTG AACTACCAGA AAACCCGGCT GGTGCAAGTG 
GGCGTCTACA ATGGCTCGCA AGTTGTAATG AACGCTCAGA GGAAGATTAT TTGGCCAGGA 
GGAGAGACTG AAAAGCCGAA AGGCTATCAG ATGTCAACTA GATTAAAGAT TGTAACAATT 
CATCAAGAGC CCTTTGTCTA CGTGAAGCCG ACTTTACGAG ATGGAACCTG TAAGGAAGAG 
CACACTGTAA ATGGAGTCAA AATCAAAAAA GTGATCTGCA CCGGCCCAAA CGAGACCATT 
CCAGGTCGCC CCATAGTGCC TCAGTGCTGC TATGGGTTCT GCATTGACCT TTTGATCAAA 
CTTGCATTCA CAATGAACTT CACCTATGAA GTGCATCTTG TGGCTGATGG CAAATTTGGC 
ACTCAGGAGC GTGTAAATAA CAGCAACAAG AAAGAGTGGA ATGGCATGAT GGGAGAGCTT 
CTGAGTGGCC TGGCAGACAT GATCGTGGCT CCACTTACAA TCAACAATGA ACGAGCCCAG 
TACATAGAGT TCTCTAAACC GTTCAAATAC CAGGGGCTCA CAATACTTGT CAAAAAGGAA 
ATACCACGCA GTACACTGGA CTCGTTCATG CAGCCCTTTC AGAGCACTCT GTGGTTACTG 
GTGGGTCTAT CGGTCCATGT TGTGGCGGTG ATGCTCTACC TTTTAGACCG TTTCAGCCCA 
TTTGGAAGGT TTAAAGTGAA TAGTGAAGAG GAAGAAGAAG ACGCCCTCAC CTTATCCTCC 
GCTATGTGGT TCTCCTGGGG TGTACTTCTG AACTCCGGCA TTGGAGAAGG TGCCCCTCGG 
AGCTTTTCGG CGAGGATTTT AGGTATGGTG TGGGCTGGTT TTGCTATGAT TATAGTTGCA 
TCTTATACTG CCAATTTGGC AGCCTTCCTA GTGCTGGATC GGCCTGAGGA GCGCATTACC 
GGCATCAACG ACCCTAGGCT GCGAAACCCA TCAGATAAGT TCATCTACGC CACAGTGAAG 
CAGAGCTCTG TGGACATTTA TTTCCGGCGC CAAGTTGAGC TTAGCACCAT GTACCGCCAC 
ATGGAAAAGC ACAACTACGA GAGCGCCGCT GAAGCCATCC AGGCTGTGCG AGACAACAAA 
CTCCACGCAT TTATATGGGA TTCAGCAGTG TTGGAGTTTG AGGCCTCGCA GAAATGTGAC 
CTGGTTACTA CGGGCGAGCT GTTCTTCCGC TCTGGATTCG GTATCGGCAT GCGTAAAGAC 
AGCCCTTGGA AGCAAAACGT CTCTCTGGCC ATCCTCAGTT CCCATGAGAA CGGCTTCATG 
GAGGACTTGG ATAAAACCTG GGTTCGATAT CAGGAGTGTG ACTCCAGAAG CAATGCCCCA 
GCCACGCTCA CTTTTGAGAA TATGGCAGGC GTGTTCATGT TGGTGGCTGG TGGCATAGTC 
GCCGGGATCT TCCTCATCTT CATTGAGATT GCATACAAAC GACACAAGGA CGCCCGCAGG 
AAGCAAATGC AGCTGGCCTT TGCAGCAGTT AACGTCTGGA GGAAGAACCT ACAGGATAGG 
AAAAGTGGTA GAGCAGAGCC CGACCCCAAA AAGAAAGCCT CTTTTAGGTC CATCAGTACC 
AACCTGGCCT CCAACATCAA GAGACGTAGG TCGTCCAAAG ACACGCCATA TCCCACGGAC 
ATTACGGGCC AGCTCAACCT GTCAGACCCC TCTGTGAGCA CAGTGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

grin1b ( XM_005171777.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171834.1 Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 1b (grin1b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa10405
Clone ID Related Accession (Same CDS sequence)	XM_005171777.1 , XM_005171777.2
Accession Version	XM_005171777.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2703bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	glutamate receptor, ionotropic, N-methyl D-aspartate 1b isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCCTGG TTCTGTTCGC CTTTCTCATC TCGTGCTCCT GCGCGAGAGG CGGATGCGAA 
CCCAAGACTG TGAACATTGG AGCTGTTCTG AGCCAGAAGA GATATGAACA AGTGTTCAAA 
GACGCCGTCA CCCAAGCCAA CAATATCTAC GGCAAAGATA AGTTCAAGAT GAACGCGATT 
TCAGTCACCC ATAAGCCAAA CGCCATCCAG ATGGCACTCT CCGTGTGCGA GGATCTCATC 
TCTAACCAGG TTTATGCGAT TTTGGTAAGC CACCCTCCCC AGTCCAATGA CCATCTGACA 
CCCACTCCGG TGTCCTACAC GGCTGGCTTT TACCGTATCC CTGTAGTGGG CCTGACCACC 
CGCATGTCCA TCTATTCTGA TAAGAGTATC CACCTCTCCT TCCTGCGCAC GGTGCCTCCC 
TATTCCCACC AAGCCCAAGT GTGGTTTGAC ATGATGCGCG AGTTCCAGTG GAATCACATC 
ATCCTGATTG TGAGCGACGA CCACGAGGGC AGAGCGGCAC AGAAAAGGCT GGAAACCCTT 
CTGGAGGAGA GGGAAACAAA GAGTAAAAAC AGGAACTATG AAAACCTCGA CCAACTGTCC 
TTTGACAACA AGCGAGGACC CAAGGCAGAG AAAGTCCTCC TGTTCAGCCA AGACACAAAT 
CTGACGGCTC TGCTCCAGGA AGCCAAGGAG CTTGAGGCCC GAGTTATCAT CCTGTCTGCA 
AGTGAAGACG AAGCCGCGGC CATTTATAAA GTGGCACGCC AGCTCAATAT GACAGGCTCT 
GGTTACGTGT GGCTGGTTGG AGAAAGGGAG ATGTCTGGTA AAGCACTGAG TGAAGCCCCA 
GATGGCTTGC TCGGCCTCCA GCTAATCAAT GGCAAGAACG AGTCTGCGCA CATCTACGAT 
GCCGTGGCTG TGGTGGCCCA GTCCATCCAG GAGCTCTTTG AGAAGGAGAA TATCACAGAG 
CCTCCTCGAG GCTGTGTTGG CAATACAAAC ATCTGGAAGA CTGGCCCACT CTTCAAACGA 
GTGCTGATGT CATCCAAGTA TCCAGATGGC CTGACAGGCC GCGTGGAGTT TAATGATGAC 
GGAGACAGGA GGTTCGCCCA CTACAGCATT CTGAACTACC AGAAAACCCG GCTGGTGCAA 
GTGGGCGTCT ACAATGGCTC GCAAGTTGTA ATGAACGCTC AGAGGAAGAT TATTTGGCCA 
GGAGGAGAGA CTGAAAAGCC GAAAGGCTAT CAGATGTCAA CTAGATTAAA GATTGTAACA 
ATTCATCAAG AGCCCTTTGT CTACGTGAAG CCGACTTTAC GAGATGGAAC CTGTAAGGAA 
GAGCACACTG TAAATGGAGT CAAAATCAAA AAAGTGATCT GCACCGGCCC AAACGAGACC 
ATTCCAGGTC GCCCCATAGT GCCTCAGTGC TGCTATGGGT TCTGCATTGA CCTTTTGATC 
AAACTTGCAT TCACAATGAA CTTCACCTAT GAAGTGCATC TTGTGGCTGA TGGCAAATTT 
GGCACTCAGG AGCGTGTAAA TAACAGCAAC AAGAAAGAGT GGAATGGCAT GATGGGAGAG 
CTTCTGAGTG GCCTGGCAGA CATGATCGTG GCTCCACTTA CAATCAACAA TGAACGAGCC 
CAGTACATAG AGTTCTCTAA ACCGTTCAAA TACCAGGGGC TCACAATACT TGTCAAAAAG 
GAAATACCAC GCAGTACACT GGACTCGTTC ATGCAGCCCT TTCAGAGCAC TCTGTGGTTA 
CTGGTGGGTC TATCGGTCCA TGTTGTGGCG GTGATGCTCT ACCTTTTAGA CCGTTTCAGC 
CCATTTGGAA GGTTTAAAGT GAATAGTGAA GAGGAAGAAG AAGACGCCCT CACCTTATCC 
TCCGCTATGT GGTTCTCCTG GGGTGTACTT CTGAACTCCG GCATTGGAGA AGGTGCCCCT 
CGGAGCTTTT CGGCGAGGAT TTTAGGTATG GTGTGGGCTG GTTTTGCTAT GATTATAGTT 
GCATCTTATA CTGCCAATTT GGCAGCCTTC CTAGTGCTGG ATCGGCCTGA GGAGCGCATT 
ACCGGCATCA ACGACCCTAG GCTGCGAAAC CCATCAGATA AGTTCATCTA CGCCACAGTG 
AAGCAGAGCT CTGTGGACAT TTATTTCCGG CGCCAAGTTG AGCTTAGCAC CATGTACCGC 
CACATGGAAA AGCACAACTA CGAGAGCGCC GCTGAAGCCA TCCAGGCTGT GCGAGACAAC 
AAACTCCACG CATTTATATG GGATTCAGCA GTGTTGGAGT TTGAGGCCTC GCAGAAATGT 
GACCTGGTTA CTACGGGCGA GCTGTTCTTC CGCTCTGGAT TCGGTATCGG CATGCGTAAA 
GACAGCCCTT GGAAGCAAAA CGTCTCTCTG GCCATCCTCA GTTCCCATGA GAACGGCTTC 
ATGGAGGACT TGGATAAAAC CTGGGTTCGA TATCAGGAGT GTGACTCCAG AAGCAATGCC 
CCAGCCACGC TCACTTTTGA GAATATGGCA GGCGTGTTCA TGTTGGTGGC TGGTGGCATA 
GTCGCCGGGA TCTTCCTCAT CTTCATTGAG ATTGCATACA AACGACACAA GGACGCCCGC 
AGGAAGCAAA TGCAGCTGGC CTTTGCAGCA GTTAACGTCT GGAGGAAGAA CCTACAGCCA 
TATCCCACGG ACATTACGGG CCAGCTCAAC CTGTCAGACC CCTCTGTGAG CACAGTGGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171834.1
CDS	601..3303
Translation	MRLVLFAFLISCSCARGGCEPKTVNIGAVLSQKRYEQVFKDAVTQANNIYGKDKFKMNAISVTHKPNAIQMALSVCEDLISNQVYAILVSHPPQSNDHLTPTPVSYTAGFYRIPVVGLTTRMSIYSDKSIHLSFLRTVPPYSHQAQVWFDMMREFQWNHIILIVSDDHEGRAAQKRLETLLEERETKSKNRNYENLDQLSFDNKRGPKAEKVLLFSQDTNLTALLQEAKELEARVIILSASEDEAAAIYKVARQLNMTGSGYVWLVGEREMSGKALSEAPDGLLGLQLINGKNESAHIYDAVAVVAQSIQELFEKENITEPPRGCVGNTNIWKTGPLFKRVLMSSKYPDGLTGRVEFNDDGDRRFAHYSILNYQKTRLVQVGVYNGSQVVMNAQRKIIWPGGETEKPKGYQMSTRLKIVTIHQEPFVYVKPTLRDGTCKEEHTVNGVKIKKVICTGPNETIPGRPIVPQCCYGFCIDLLIKLAFTMNFTYEVHLVADGKFGTQERVNNSNKKEWNGMMGELLSGLADMIVAPLTINNERAQYIEFSKPFKYQGLTILVKKEIPRSTLDSFMQPFQSTLWLLVGLSVHVVAVMLYLLDRFSPFGRFKVNSEEEEEDALTLSSAMWFSWGVLLNSGIGEGAPRSFSARILGMVWAGFAMIIVASYTANLAAFLVLDRPEERITGINDPRLRNPSDKFIYATVKQSSVDIYFRRQVELSTMYRHMEKHNYESAAEAIQAVRDNKLHAFIWDSAVLEFEASQKCDLVTTGELFFRSGFGIGMRKDSPWKQNVSLAILSSHENGFMEDLDKTWVRYQECDSRSNAPATLTFENMAGVFMLVAGGIVAGIFLIFIEIAYKRHKDARRKQMQLAFAAVNVWRKNLQPYPTDITGQLNLSDPSVSTVV

Target ORF information:

RefSeq Version	XM_005171777.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 1b (grin1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171777.1

ORF Insert Sequence:

ATGCGCCTGG TTCTGTTCGC CTTTCTCATC TCGTGCTCCT GCGCGAGAGG CGGATGCGAA 
CCCAAGACTG TGAACATTGG AGCTGTTCTG AGCCAGAAGA GATATGAACA AGTGTTCAAA 
GACGCCGTCA CCCAAGCCAA CAATATCTAC GGCAAAGATA AGTTCAAGAT GAACGCGATT 
TCAGTCACCC ATAAGCCAAA CGCCATCCAG ATGGCACTCT CCGTGTGCGA GGATCTCATC 
TCTAACCAGG TTTATGCGAT TTTGGTAAGC CACCCTCCCC AGTCCAATGA CCATCTGACA 
CCCACTCCGG TGTCCTACAC GGCTGGCTTT TACCGTATCC CTGTAGTGGG CCTGACCACC 
CGCATGTCCA TCTATTCTGA TAAGAGTATC CACCTCTCCT TCCTGCGCAC GGTGCCTCCC 
TATTCCCACC AAGCCCAAGT GTGGTTTGAC ATGATGCGCG AGTTCCAGTG GAATCACATC 
ATCCTGATTG TGAGCGACGA CCACGAGGGC AGAGCGGCAC AGAAAAGGCT GGAAACCCTT 
CTGGAGGAGA GGGAAACAAA GAGTAAAAAC AGGAACTATG AAAACCTCGA CCAACTGTCC 
TTTGACAACA AGCGAGGACC CAAGGCAGAG AAAGTCCTCC TGTTCAGCCA AGACACAAAT 
CTGACGGCTC TGCTCCAGGA AGCCAAGGAG CTTGAGGCCC GAGTTATCAT CCTGTCTGCA 
AGTGAAGACG AAGCCGCGGC CATTTATAAA GTGGCACGCC AGCTCAATAT GACAGGCTCT 
GGTTACGTGT GGCTGGTTGG AGAAAGGGAG ATGTCTGGTA AAGCACTGAG TGAAGCCCCA 
GATGGCTTGC TCGGCCTCCA GCTAATCAAT GGCAAGAACG AGTCTGCGCA CATCTACGAT 
GCCGTGGCTG TGGTGGCCCA GTCCATCCAG GAGCTCTTTG AGAAGGAGAA TATCACAGAG 
CCTCCTCGAG GCTGTGTTGG CAATACAAAC ATCTGGAAGA CTGGCCCACT CTTCAAACGA 
GTGCTGATGT CATCCAAGTA TCCAGATGGC CTGACAGGCC GCGTGGAGTT TAATGATGAC 
GGAGACAGGA GGTTCGCCCA CTACAGCATT CTGAACTACC AGAAAACCCG GCTGGTGCAA 
GTGGGCGTCT ACAATGGCTC GCAAGTTGTA ATGAACGCTC AGAGGAAGAT TATTTGGCCA 
GGAGGAGAGA CTGAAAAGCC GAAAGGCTAT CAGATGTCAA CTAGATTAAA GATTGTAACA 
ATTCATCAAG AGCCCTTTGT CTACGTGAAG CCGACTTTAC GAGATGGAAC CTGTAAGGAA 
GAGCACACTG TAAATGGAGT CAAAATCAAA AAAGTGATCT GCACCGGCCC AAACGAGACC 
ATTCCAGGTC GCCCCATAGT GCCTCAGTGC TGCTATGGGT TCTGCATTGA CCTTTTGATC 
AAACTTGCAT TCACAATGAA CTTCACCTAT GAAGTGCATC TTGTGGCTGA TGGCAAATTT 
GGCACTCAGG AGCGTGTAAA TAACAGCAAC AAGAAAGAGT GGAATGGCAT GATGGGAGAG 
CTTCTGAGTG GCCTGGCAGA CATGATCGTG GCTCCACTTA CAATCAACAA TGAACGAGCC 
CAGTACATAG AGTTCTCTAA ACCGTTCAAA TACCAGGGGC TCACAATACT TGTCAAAAAG 
GAAATACCAC GCAGTACACT GGACTCGTTC ATGCAGCCCT TTCAGAGCAC TCTGTGGTTA 
CTGGTGGGTC TATCGGTCCA TGTTGTGGCG GTGATGCTCT ACCTTTTAGA CCGTTTCAGC 
CCATTTGGAA GGTTTAAAGT GAATAGTGAA GAGGAAGAAG AAGACGCCCT CACCTTATCC 
TCCGCTATGT GGTTCTCCTG GGGTGTACTT CTGAACTCCG GCATTGGAGA AGGTGCCCCT 
CGGAGCTTTT CGGCGAGGAT TTTAGGTATG GTGTGGGCTG GTTTTGCTAT GATTATAGTT 
GCATCTTATA CTGCCAATTT GGCAGCCTTC CTAGTGCTGG ATCGGCCTGA GGAGCGCATT 
ACCGGCATCA ACGACCCTAG GCTGCGAAAC CCATCAGATA AGTTCATCTA CGCCACAGTG 
AAGCAGAGCT CTGTGGACAT TTATTTCCGG CGCCAAGTTG AGCTTAGCAC CATGTACCGC 
CACATGGAAA AGCACAACTA CGAGAGCGCC GCTGAAGCCA TCCAGGCTGT GCGAGACAAC 
AAACTCCACG CATTTATATG GGATTCAGCA GTGTTGGAGT TTGAGGCCTC GCAGAAATGT 
GACCTGGTTA CTACGGGCGA GCTGTTCTTC CGCTCTGGAT TCGGTATCGG CATGCGTAAA 
GACAGCCCTT GGAAGCAAAA CGTCTCTCTG GCCATCCTCA GTTCCCATGA GAACGGCTTC 
ATGGAGGACT TGGATAAAAC CTGGGTTCGA TATCAGGAGT GTGACTCCAG AAGCAATGCC 
CCAGCCACGC TCACTTTTGA GAATATGGCA GGCGTGTTCA TGTTGGTGGC TGGTGGCATA 
GTCGCCGGGA TCTTCCTCAT CTTCATTGAG ATTGCATACA AACGACACAA GGACGCCCGC 
AGGAAGCAAA TGCAGCTGGC CTTTGCAGCA GTTAACGTCT GGAGGAAGAA CCTACAGCCA 
TATCCCACGG ACATTACGGG CCAGCTCAAC CTGTCAGACC CCTCTGTGAG CACAGTGGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

grin1b ( XM_005171777.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171834.1 Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 1b (grin1b), transcript variant X2, mRNA.

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CloneID	ODa10405
Clone ID Related Accession (Same CDS sequence)	XM_005171777.1 , XM_005171777.2
Accession Version	XM_005171777.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2703bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	glutamate receptor, ionotropic, N-methyl D-aspartate 1b isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005171777.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCCTGG TTCTGTTCGC CTTTCTCATC TCGTGCTCCT GCGCGAGAGG CGGATGCGAA 
CCCAAGACTG TGAACATTGG AGCTGTTCTG AGCCAGAAGA GATATGAACA AGTGTTCAAA 
GACGCCGTCA CCCAAGCCAA CAATATCTAC GGCAAAGATA AGTTCAAGAT GAACGCGATT 
TCAGTCACCC ATAAGCCAAA CGCCATCCAG ATGGCACTCT CCGTGTGCGA GGATCTCATC 
TCTAACCAGG TTTATGCGAT TTTGGTAAGC CACCCTCCCC AGTCCAATGA CCATCTGACA 
CCCACTCCGG TGTCCTACAC GGCTGGCTTT TACCGTATCC CTGTAGTGGG CCTGACCACC 
CGCATGTCCA TCTATTCTGA TAAGAGTATC CACCTCTCCT TCCTGCGCAC GGTGCCTCCC 
TATTCCCACC AAGCCCAAGT GTGGTTTGAC ATGATGCGCG AGTTCCAGTG GAATCACATC 
ATCCTGATTG TGAGCGACGA CCACGAGGGC AGAGCGGCAC AGAAAAGGCT GGAAACCCTT 
CTGGAGGAGA GGGAAACAAA GAGTAAAAAC AGGAACTATG AAAACCTCGA CCAACTGTCC 
TTTGACAACA AGCGAGGACC CAAGGCAGAG AAAGTCCTCC TGTTCAGCCA AGACACAAAT 
CTGACGGCTC TGCTCCAGGA AGCCAAGGAG CTTGAGGCCC GAGTTATCAT CCTGTCTGCA 
AGTGAAGACG AAGCCGCGGC CATTTATAAA GTGGCACGCC AGCTCAATAT GACAGGCTCT 
GGTTACGTGT GGCTGGTTGG AGAAAGGGAG ATGTCTGGTA AAGCACTGAG TGAAGCCCCA 
GATGGCTTGC TCGGCCTCCA GCTAATCAAT GGCAAGAACG AGTCTGCGCA CATCTACGAT 
GCCGTGGCTG TGGTGGCCCA GTCCATCCAG GAGCTCTTTG AGAAGGAGAA TATCACAGAG 
CCTCCTCGAG GCTGTGTTGG CAATACAAAC ATCTGGAAGA CTGGCCCACT CTTCAAACGA 
GTGCTGATGT CATCCAAGTA TCCAGATGGC CTGACAGGCC GCGTGGAGTT TAATGATGAC 
GGAGACAGGA GGTTCGCCCA CTACAGCATT CTGAACTACC AGAAAACCCG GCTGGTGCAA 
GTGGGCGTCT ACAATGGCTC GCAAGTTGTA ATGAACGCTC AGAGGAAGAT TATTTGGCCA 
GGAGGAGAGA CTGAAAAGCC GAAAGGCTAT CAGATGTCAA CTAGATTAAA GATTGTAACA 
ATTCATCAAG AGCCCTTTGT CTACGTGAAG CCGACTTTAC GAGATGGAAC CTGTAAGGAA 
GAGCACACTG TAAATGGAGT CAAAATCAAA AAAGTGATCT GCACCGGCCC AAACGAGACC 
ATTCCAGGTC GCCCCATAGT GCCTCAGTGC TGCTATGGGT TCTGCATTGA CCTTTTGATC 
AAACTTGCAT TCACAATGAA CTTCACCTAT GAAGTGCATC TTGTGGCTGA TGGCAAATTT 
GGCACTCAGG AGCGTGTAAA TAACAGCAAC AAGAAAGAGT GGAATGGCAT GATGGGAGAG 
CTTCTGAGTG GCCTGGCAGA CATGATCGTG GCTCCACTTA CAATCAACAA TGAACGAGCC 
CAGTACATAG AGTTCTCTAA ACCGTTCAAA TACCAGGGGC TCACAATACT TGTCAAAAAG 
GAAATACCAC GCAGTACACT GGACTCGTTC ATGCAGCCCT TTCAGAGCAC TCTGTGGTTA 
CTGGTGGGTC TATCGGTCCA TGTTGTGGCG GTGATGCTCT ACCTTTTAGA CCGTTTCAGC 
CCATTTGGAA GGTTTAAAGT GAATAGTGAA GAGGAAGAAG AAGACGCCCT CACCTTATCC 
TCCGCTATGT GGTTCTCCTG GGGTGTACTT CTGAACTCCG GCATTGGAGA AGGTGCCCCT 
CGGAGCTTTT CGGCGAGGAT TTTAGGTATG GTGTGGGCTG GTTTTGCTAT GATTATAGTT 
GCATCTTATA CTGCCAATTT GGCAGCCTTC CTAGTGCTGG ATCGGCCTGA GGAGCGCATT 
ACCGGCATCA ACGACCCTAG GCTGCGAAAC CCATCAGATA AGTTCATCTA CGCCACAGTG 
AAGCAGAGCT CTGTGGACAT TTATTTCCGG CGCCAAGTTG AGCTTAGCAC CATGTACCGC 
CACATGGAAA AGCACAACTA CGAGAGCGCC GCTGAAGCCA TCCAGGCTGT GCGAGACAAC 
AAACTCCACG CATTTATATG GGATTCAGCA GTGTTGGAGT TTGAGGCCTC GCAGAAATGT 
GACCTGGTTA CTACGGGCGA GCTGTTCTTC CGCTCTGGAT TCGGTATCGG CATGCGTAAA 
GACAGCCCTT GGAAGCAAAA CGTCTCTCTG GCCATCCTCA GTTCCCATGA GAACGGCTTC 
ATGGAGGACT TGGATAAAAC CTGGGTTCGA TATCAGGAGT GTGACTCCAG AAGCAATGCC 
CCAGCCACGC TCACTTTTGA GAATATGGCA GGCGTGTTCA TGTTGGTGGC TGGTGGCATA 
GTCGCCGGGA TCTTCCTCAT CTTCATTGAG ATTGCATACA AACGACACAA GGACGCCCGC 
AGGAAGCAAA TGCAGCTGGC CTTTGCAGCA GTTAACGTCT GGAGGAAGAA CCTACAGCCA 
TATCCCACGG ACATTACGGG CCAGCTCAAC CTGTCAGACC CCTCTGTGAG CACAGTGGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171834.1
CDS	1..2703
Translation	MRLVLFAFLISCSCARGGCEPKTVNIGAVLSQKRYEQVFKDAVTQANNIYGKDKFKMNAISVTHKPNAIQMALSVCEDLISNQVYAILVSHPPQSNDHLTPTPVSYTAGFYRIPVVGLTTRMSIYSDKSIHLSFLRTVPPYSHQAQVWFDMMREFQWNHIILIVSDDHEGRAAQKRLETLLEERETKSKNRNYENLDQLSFDNKRGPKAEKVLLFSQDTNLTALLQEAKELEARVIILSASEDEAAAIYKVARQLNMTGSGYVWLVGEREMSGKALSEAPDGLLGLQLINGKNESAHIYDAVAVVAQSIQELFEKENITEPPRGCVGNTNIWKTGPLFKRVLMSSKYPDGLTGRVEFNDDGDRRFAHYSILNYQKTRLVQVGVYNGSQVVMNAQRKIIWPGGETEKPKGYQMSTRLKIVTIHQEPFVYVKPTLRDGTCKEEHTVNGVKIKKVICTGPNETIPGRPIVPQCCYGFCIDLLIKLAFTMNFTYEVHLVADGKFGTQERVNNSNKKEWNGMMGELLSGLADMIVAPLTINNERAQYIEFSKPFKYQGLTILVKKEIPRSTLDSFMQPFQSTLWLLVGLSVHVVAVMLYLLDRFSPFGRFKVNSEEEEEDALTLSSAMWFSWGVLLNSGIGEGAPRSFSARILGMVWAGFAMIIVASYTANLAAFLVLDRPEERITGINDPRLRNPSDKFIYATVKQSSVDIYFRRQVELSTMYRHMEKHNYESAAEAIQAVRDNKLHAFIWDSAVLEFEASQKCDLVTTGELFFRSGFGIGMRKDSPWKQNVSLAILSSHENGFMEDLDKTWVRYQECDSRSNAPATLTFENMAGVFMLVAGGIVAGIFLIFIEIAYKRHKDARRKQMQLAFAAVNVWRKNLQPYPTDITGQLNLSDPSVSTVV

Target ORF information:

RefSeq Version	XM_005171777.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio glutamate receptor, ionotropic, N-methyl D-aspartate 1b (grin1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171777.2

ORF Insert Sequence:

ATGCGCCTGG TTCTGTTCGC CTTTCTCATC TCGTGCTCCT GCGCGAGAGG CGGATGCGAA 
CCCAAGACTG TGAACATTGG AGCTGTTCTG AGCCAGAAGA GATATGAACA AGTGTTCAAA 
GACGCCGTCA CCCAAGCCAA CAATATCTAC GGCAAAGATA AGTTCAAGAT GAACGCGATT 
TCAGTCACCC ATAAGCCAAA CGCCATCCAG ATGGCACTCT CCGTGTGCGA GGATCTCATC 
TCTAACCAGG TTTATGCGAT TTTGGTAAGC CACCCTCCCC AGTCCAATGA CCATCTGACA 
CCCACTCCGG TGTCCTACAC GGCTGGCTTT TACCGTATCC CTGTAGTGGG CCTGACCACC 
CGCATGTCCA TCTATTCTGA TAAGAGTATC CACCTCTCCT TCCTGCGCAC GGTGCCTCCC 
TATTCCCACC AAGCCCAAGT GTGGTTTGAC ATGATGCGCG AGTTCCAGTG GAATCACATC 
ATCCTGATTG TGAGCGACGA CCACGAGGGC AGAGCGGCAC AGAAAAGGCT GGAAACCCTT 
CTGGAGGAGA GGGAAACAAA GAGTAAAAAC AGGAACTATG AAAACCTCGA CCAACTGTCC 
TTTGACAACA AGCGAGGACC CAAGGCAGAG AAAGTCCTCC TGTTCAGCCA AGACACAAAT 
CTGACGGCTC TGCTCCAGGA AGCCAAGGAG CTTGAGGCCC GAGTTATCAT CCTGTCTGCA 
AGTGAAGACG AAGCCGCGGC CATTTATAAA GTGGCACGCC AGCTCAATAT GACAGGCTCT 
GGTTACGTGT GGCTGGTTGG AGAAAGGGAG ATGTCTGGTA AAGCACTGAG TGAAGCCCCA 
GATGGCTTGC TCGGCCTCCA GCTAATCAAT GGCAAGAACG AGTCTGCGCA CATCTACGAT 
GCCGTGGCTG TGGTGGCCCA GTCCATCCAG GAGCTCTTTG AGAAGGAGAA TATCACAGAG 
CCTCCTCGAG GCTGTGTTGG CAATACAAAC ATCTGGAAGA CTGGCCCACT CTTCAAACGA 
GTGCTGATGT CATCCAAGTA TCCAGATGGC CTGACAGGCC GCGTGGAGTT TAATGATGAC 
GGAGACAGGA GGTTCGCCCA CTACAGCATT CTGAACTACC AGAAAACCCG GCTGGTGCAA 
GTGGGCGTCT ACAATGGCTC GCAAGTTGTA ATGAACGCTC AGAGGAAGAT TATTTGGCCA 
GGAGGAGAGA CTGAAAAGCC GAAAGGCTAT CAGATGTCAA CTAGATTAAA GATTGTAACA 
ATTCATCAAG AGCCCTTTGT CTACGTGAAG CCGACTTTAC GAGATGGAAC CTGTAAGGAA 
GAGCACACTG TAAATGGAGT CAAAATCAAA AAAGTGATCT GCACCGGCCC AAACGAGACC 
ATTCCAGGTC GCCCCATAGT GCCTCAGTGC TGCTATGGGT TCTGCATTGA CCTTTTGATC 
AAACTTGCAT TCACAATGAA CTTCACCTAT GAAGTGCATC TTGTGGCTGA TGGCAAATTT 
GGCACTCAGG AGCGTGTAAA TAACAGCAAC AAGAAAGAGT GGAATGGCAT GATGGGAGAG 
CTTCTGAGTG GCCTGGCAGA CATGATCGTG GCTCCACTTA CAATCAACAA TGAACGAGCC 
CAGTACATAG AGTTCTCTAA ACCGTTCAAA TACCAGGGGC TCACAATACT TGTCAAAAAG 
GAAATACCAC GCAGTACACT GGACTCGTTC ATGCAGCCCT TTCAGAGCAC TCTGTGGTTA 
CTGGTGGGTC TATCGGTCCA TGTTGTGGCG GTGATGCTCT ACCTTTTAGA CCGTTTCAGC 
CCATTTGGAA GGTTTAAAGT GAATAGTGAA GAGGAAGAAG AAGACGCCCT CACCTTATCC 
TCCGCTATGT GGTTCTCCTG GGGTGTACTT CTGAACTCCG GCATTGGAGA AGGTGCCCCT 
CGGAGCTTTT CGGCGAGGAT TTTAGGTATG GTGTGGGCTG GTTTTGCTAT GATTATAGTT 
GCATCTTATA CTGCCAATTT GGCAGCCTTC CTAGTGCTGG ATCGGCCTGA GGAGCGCATT 
ACCGGCATCA ACGACCCTAG GCTGCGAAAC CCATCAGATA AGTTCATCTA CGCCACAGTG 
AAGCAGAGCT CTGTGGACAT TTATTTCCGG CGCCAAGTTG AGCTTAGCAC CATGTACCGC 
CACATGGAAA AGCACAACTA CGAGAGCGCC GCTGAAGCCA TCCAGGCTGT GCGAGACAAC 
AAACTCCACG CATTTATATG GGATTCAGCA GTGTTGGAGT TTGAGGCCTC GCAGAAATGT 
GACCTGGTTA CTACGGGCGA GCTGTTCTTC CGCTCTGGAT TCGGTATCGG CATGCGTAAA 
GACAGCCCTT GGAAGCAAAA CGTCTCTCTG GCCATCCTCA GTTCCCATGA GAACGGCTTC 
ATGGAGGACT TGGATAAAAC CTGGGTTCGA TATCAGGAGT GTGACTCCAG AAGCAATGCC 
CCAGCCACGC TCACTTTTGA GAATATGGCA GGCGTGTTCA TGTTGGTGGC TGGTGGCATA 
GTCGCCGGGA TCTTCCTCAT CTTCATTGAG ATTGCATACA AACGACACAA GGACGCCCGC 
AGGAAGCAAA TGCAGCTGGC CTTTGCAGCA GTTAACGTCT GGAGGAAGAA CCTACAGCCA 
TATCCCACGG ACATTACGGG CCAGCTCAAC CTGTCAGACC CCTCTGTGAG CACAGTGGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pla2g6 Deficiency in Zebrafish Leads to Dopaminergic Cell Death, Axonal Degeneration, Increased ?-Synuclein Expression, and Defects in Brain Functions and Pathways.
Molecular neurobiology(2018 Jan)
S?nchez E,Azcona LJ,Pais?n-Ruiz C

Effects of 2,2',4,4'-tetrabromodiphenyl ether on neurobehavior and memory change and bcl-2, c-fos, grin1b and lingo1b gene expression in male zebrafish (Danio rerio).
Toxicology and applied pharmacology33310-16(2017 10)
Zheng S,Liu C,Huang Y,Bao M,Huang Y,Wu K

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grin1b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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