GREB1L cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	GREB1L
Entrez Gene ID	100005373
Full Name	growth regulation by estrogen in breast cancer-like
Synonyms	si:dkey-203o21.1,si:dkey-264l15.1,wu:fc65f09
General protein information	Preferred Names growth regulation by estrogen in breast cancer-like Names GREB1-like protein fc65f09
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001344416.8	XP_001344452.4	GREB1-like protein
XM_009298040.1	XP_009296315.1	GREB1-like protein
XM_009298040.2	XP_009296315.1	GREB1-like protein
XM_021466912.1	XP_021322587.1	GREB1-like protein
NM_001362307.1	NP_001349236.1	GREB1-like protein
XM_001344416.7	XP_001344452.4	GREB1-like protein

Pathways from BioSystems

Homologies

Homo sapiens (human)	GREB1L	NP_001136438.1
Gallus gallus (chicken)	GREB1L	XP_004939815.1
Pan troglodytes (chimpanzee)	GREB1L	XP_001152115.2
Canis lupus familiaris (dog)	GREB1L	XP_005623067.1
Rattus norvegicus (Norway rat)	Greb1l	XP_006254479.1
Xenopus tropicalis (tropical clawed frog)	greb1l	NP_001123751.1
	GREB1L	XP_002800930.1
Bos taurus (cattle)	GREB1L	XP_002697775.2
Mus musculus (house mouse)	Greb1l	NP_001077097.1
Danio rerio (zebrafish)	greb1l	XP_001344452.4

Gene Ontology
Bibliography

Related articles in PubMed

Exome-wide Association Study Identifies GREB1L Mutations in Congenital Kidney Malformations.
Sanna-Cherchi S, Khan K, Westland R, Krithivasan P, Fievet L, Rasouly HM, Ionita-Laza I, Capone VP, Fasel DA, Kiryluk K, Kamalakaran S, Bodria M, Otto EA, Sampson MG, Gillies CE, Vega-Warner V, Vukojevic K, Pediaditakis I, Makar GS, Mitrotti A, Verbitsky M, Martino J, Liu Q, Na YJ, Goj V, Ardissino G, Gigante M, Gesualdo L, Janezcko M, Zaniew M, Mendelsohn CL, Shril S, Hildebrandt F, van Wijk JAE, Arapovic A, Saraga M, Allegri L, Izzi C, Scolari F, Tasic V, Ghiggeri GM, Latos-Bielenska A, Materna-Kiryluk A, Mane S, Goldstein DB, Lifton RP, Katsanis N, Davis EE, Gharavi AG
American journal of human genetics101(5)789-802(2017 Nov)

A Gene Implicated in Activation of Retinoic Acid Receptor Targets Is a Novel Renal Agenesis Gene in Humans.
Brophy PD, Rasmussen M, Parida M, Bonde G, Darbro BW, Hong X, Clarke JC, Peterson KA, Denegre J, Schneider M, Sussman CR, Sunde L, Lildballe DL, Hertz JM, Cornell RA, Murray SA, Manak JR
Genetics207(1)215-228(2017 09)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

mafba is a downstream transcriptional effector of Vegfc signaling essential for embryonic lymphangiogenesis in zebrafish.
Koltowska K, Paterson S, Bower NI, Baillie GJ, Lagendijk AK, Astin JW, Chen H, Francois M, Crosier PS, Taft RJ, Simons C, Smith KA, Hogan BM
Genes & development29(15)1618-30(2015 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GREB1L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GREB1L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa06787	NM_001362307.1 Latest version!	Danio rerio GREB1 like retinoic acid receptor coactivator (greb1l), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa06787	XM_001344416.8 Latest version!	Danio rerio growth regulation by estrogen in breast cancer-like (greb1l), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa06787	XM_009298040.2 Latest version!	Danio rerio growth regulation by estrogen in breast cancer-like (greb1l), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa06787	XM_021466912.1 Latest version!	Danio rerio growth regulation by estrogen in breast cancer-like (greb1l), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
ODa06787	XM_009298040.1	Danio rerio growth regulation by estrogen in breast cancer-like (greb1l), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa06787	XM_001344416.7	Danio rerio growth regulation by estrogen in breast cancer-like (greb1l), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GREB1L ( NM_001362307.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001349236.1 Danio rerio GREB1 like retinoic acid receptor coactivator (greb1l), mRNA.

Price & Availability↑

CloneID	ODa06787
Clone ID Related Accession (Same CDS sequence)	XM_001344416.8 , XM_009298040.1 , XM_009298040.2 , XM_021466912.1 , NM_001362307.1 , XM_001344416.7
Accession Version	NM_001362307.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	GREB1-like protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT573467.8, CU138514.8 and CU682364.6. On Apr 12, 2018 this sequence version replaced XM_001344416.8. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GFIL01011944.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA898401, SAMEA898403 [ECO:0000348] ##Evidence-Data-END##

ATGGGAAATT CATACGCGGG GCAGCTGAAA TCTGCTCGCT TTGAGGAAGC TCTCCATAAC 
TCAATAGAGG CTTCATTAAG GTCAAGCGGT GGTGACCCAC AGCCGGTTTT CACACAGCTA 
TATCTCGAGC CTGATCAGTA TTCTGGCCAT GTAGAAGATA TTAAGCCCAA AATGGACCTA 
AGCCTGAGAT CCGACCCATC CACCCATGTG CTTGTGAAAT GCCACTCTTC AAATTCTGTA 
GAGGATATGG ATGACGAGGA CGATTCAGAC ACCAGCAGCC CACCATTGCC TTACCTGCAG 
GGACCCCCTC CTGATGGCTG CTGCACTGTT GATGGTTTCT GTCAAGCAGG AAAAGATCTG 
CGCCTGGTTT CCATGGCAAC AGAGTCTATC GAGGTTCCTG CAGGGTTTGA GCTGGTCGGT 
GCCAAATCGC CCAGCATCCC AGAGCACATC CTCGTCTGTG CAGTGGACAA GCGCTTCCTG 
CCAGATGAGA ATGGGAAAAA TGCACTTTTA GGGTTTTCAG GGAACTGCGT GGGTTGTGGT 
GAGAAAGGGT TCCGTTACTT CACCGAGTTC TCTAACCACA TTAACCTGAA GTTATCCACT 
CAGCCGAAGA AGCAGAAGCA CTTAAAGTAC TACCTGGTGA AGAACTCTCA GGGTGCTCTG 
TGCAAAGGAG CCCTCATTTG CTGGAAAGAT TGTAAGACAC GACCATTCTC AAACAGTGCT 
TCTTCATCCA AGCCTAGTTC CTCATCCTCA CTAAGCAGCA AAGAAAATGG AGACACAAAT 
GGCCACAGTC CATCTCCCTT CCCCCTCTCA GACTCGCCTC CTGCCCGCAT GCAATCTGGC 
TCATCCTCAG GGATTTTTGG ACCCCAGGAG CTGGGCTTCC TTAAACCATT GAACACACCG 
ACCCACGGGA CCAAGACATT ACCGATCGTT CCCACTGCCC TCCGAGTGAA TGGTCTAACT 
AATGGTCTCA GTATGGATGG ACGCTCCACT CTACTGAGCC CACCTCGTAC AAACCCACTC 
TCCACCCCTA GTCATGGCTA TAGAACCACA GAAACAGGAG ACAGTCCAGC ATCCACTGCT 
ATGTCGACAG GTCCACCAAA AAAGCGGCAT CGTAGTTGGC ATCCCACCAC TTTAGTGCCC 
ATCCCAGCCA CAGCTGTTCC TGTACCAGCC ATCCGACCGC TGACCTGCAG CTCTGGTCCT 
TTATTGTCAC TGTCCAATCA ACAGCCTGCA TCGGTGTCAG GGGTCATACA ACCACAGCCA 
ATCACAGCTG GAGAAACGGT CATCATTCCT GATAACCTTC TGAACTCTTA CGGGGTCCGG 
CCAGTCCTTC TCATTGGTCA AGGCACTTTA CCATATTTCT TTGGCAATGT GGGTGATCTG 
GTGGTGAGCC CCCTGCTGGT GAGCTGCTAC AAGGGCAGAG AGCTCAACGA GAAGACCCTG 
GCCAGCCTGG GCATGTCAGC AAACCAGTTA CTGACCACAG AGACCATGAT ACTGCTCACA 
CTGCAGTACC TCGCTCGACT GGGTACAGAG CAGATACCGT TACGGGAAGA GTTTGAGCAA 
ATCATGTTGA AGGCTATGCT GTGTGGTCCG ACTGGCCCTC CAGTGTCGCC TGCGCAGCTG 
CCCTGGTTAG CCCGAATGGA GGCCAGTGTG TCAGGGGGCA GTGTTCAGGT GCTGGTCACC 
CACGGCTCTC TTGGTGAGGG TATCTCTGAG TCTCTGCGCT CACTGAGTGA AACATCACCA 
CAGCAGCAGC AGTGTCTCCC CAACTATGTG CTCATTATCT GCACCTCCAA AAGTGGAGCC 
AATGAGTTCT GTGTGCTCGT GTTGGGTAAG TATCAGTCTC GAGCCTTGGC AGAGAGCATG 
CTGTCTACAA ACGAGTTTCT GAAGGAGATC AGTTATGAGC TCATCACAGG GAAAGTTAGT 
GTACTAGCCT CACACTTCCA GAGCACCTCT TTAGGTGACA ATATGGACAA ACAGCTGGTT 
CGTTATCAAC GTAAACGCAA AGACAGGGTG GTTCAACCTT TTCAGGGGCA TCTCACCGAG 
TACATCCACT CCCAGGAAGC TGCGACCATG ATACCAGAAT CAGGGCCAGA TCTGCTGAGC 
GATGACTTTC AGATACACCC TCCTCAGTTG AGCGTTGCAC GAAGCCTTTT GTCACAGGTT 
TGTGCTATTG CCGACTCAGG GAGCCAGAGC TTAGATCTGG GCCGATTCTG CAAAGTAGAC 
TTTCTGATTC TGGTGCCGCC CTCCCATGTT CTGGTTCACC AAACAGTCCA GCGAATACGA 
CAATCAGGCG TCCTAATAGA TCTCGGTATA GAGGACGTGT CATTAGCCAT GCAGAAGTCT 
GACAAGTATG TGGTGCGTCT GGACACAGAG GTCCACACAA AAATGGAGGC CTTTATGAGG 
AAGGTCAAGC AGAACCCTTA TACACTCTTC GTCCTCATTC ATGACAACTC GCACGTGGAC 
TTGACAAGTG CTCTTTCAGG GTCGGTTTGT CATGGGGAGC TGCAGGGATT GGCTGATCGC 
GTGGTGAACT GTCCAGAGGT TCTGGAAGCA ATAAACTTAC TGGTGCTGCA GGTCAGCTGC 
TTCCCGTTCA CCCTGCAGAG CCGGCAGTCA CGAATCAGTA CCCAGAATGA AGTGCACTGG 
CCCGACACTG AGAACCAGCA GGGTGAAGCT TCTCCTAAAG ACTTGATCTA CTTTGGGCTG 
AAGGACTACA GCAAGTCTCT GCAGTGGGGA GTGGCGAGTC CAATTCTGCG ATGTGATGAC 
GCCTTTGAGC GGATGGTTAA AACCCTGTTG GAGAGGCACC CACATTTGCA CAGTATGGTG 
ATCCGCAGCT ACCTGTTGAT CCAGCAGTAC ACAGAGGCTC TGATGGCCCT CACAGCGGCC 
CCTTCTTTGC GGGATCACGT GACCCCTCAG ACACTTGCCA TGGTGGAGGA TCTGCTGAGT 
GTGCCAGGCC GCAGCAAGCA TGGGTGCGGA CACATGCTGC TGGTGCGTGT GCCTTCTCTG 
CAGCTGGCAC GACTGGCACA GGAGCGGCTG GAGGAGGCTC GGGACAAGCT GGGGCTGCAG 
TACCGCTTTG CCGTGCTGCT AGGAAGCCCT GCTGCGGAGA TCAGCCTGCC GGTGCACTTC 
TGTGCCCGTC TGCGGGCTTG GAGGGGCTGT AAAAATGAGG AGTGGGTCCC ACATACATAT 
GAAGATCTGG AAGGACTTCC ATGCATTGTC ATCCTCACTG GAAAAGATCC TCTTGGAGAA 
ACCTTCCCGA GGTCTCTAAA GTACTGTGAC TTGCGGCTGA TTGACTCCAG CTACCTGACA 
CGCACAGCGC TGGAACAGGA AGTGGGTTTG GCCTGTTCAT ATGTGACACG TAGAGTAATC 
CCTAAGACCA AAACTGCCAC AAGCAGAGAA GAGAGACCGA GAGAAGGTGA AAGGAGCTCT 
GGAGAAACAG CAGAACACGA TGATCTCCCT ATGGAGCTGG AAAGACCACC CAGCAATGCC 
AGCGCTGCTA CTAGGACCTC CGGCTCCACC ACAGAAAATG GTGTCAGTTC CTCAAGTATA 
CTTGACAAGC CATCCTCGCA GAGTGACCCT TGTGGAAGCA GGACTATGAT GGACTCTTGC 
TCCTCTCCTG TTCGCTTCAA GCAGGAGTGC GACTCTCAGG CTCCCTCATC ATCCAGCACG 
TCCTCATTCT CCTCTGCTTC CTCCTCCTCT TCTTCTTCCT CGTCTCCGGC AGCTCAGAGA 
CCGAGTCAGT CTACACAGGC TCCACGTGAA TGTAACAGGA CACAGGTCTT TCCCCGGACG 
GCGGTGCTGT CTCGGGCTGC GTATACCCTG CTGGCTCCTG AAACTCTGGG TCATCCCAGT 
TCTGCCTCTC TGCTGCCACA TGCGGATGTG TCGTGGAGCA GTCCGCTCAG ACCACCGGTT 
CCTCATGGCC TTGGTGGAGC AGAGCAGTCC CTCTACTATC GTCAGTGGAC CACAGCCAGG 
CAACACCACG CTGATTATGA AGGCCCAGTA CCCCATCCCC ACCCAAGAAG ACTGCTTCTG 
AGCGGCCCAC CACAGGTTGG AAAAACAGGT GCATATCTGC AGTTCCTACG AATTCTGTTC 
CGTATGCTGA TTCGCCTTCT GGAGGTAGAT GTTTATGATG AAGAAGAGCT GGAGGAAGAT 
GTGCAAGACA AATCAAAGGT TCCCCCTTCT AGTGGGCCAC AGTGGCCTGA TGTGGAGGAC 
GTCCGCAAAC TAAGATTTGA CCTCTGCCCT CATGACTGCA AGTTCAAATA TTCAAGTCCA 
GTGTATGCCA ACAGGATGCC AAAAACACAG AGTGGGGTTA AAACCGAAAG ACTGGATACA 
GAAGCGGACC CACCAAAGAG AAACACAGTA TCTGTTAGAC TCAGCCTTTT TGCAGCCCAC 
AATGCATTTC ACCACTGTGA GCAGTGCCAC CATTACAGTG AACCTATTCC TGCTGCTCAG 
CTCTCTGACT GCACCTTCCA CGCTTTCACC TTTTGCTCAT CTATGCTGGG AGAGGAAGTC 
CAGCTGCACT TTATCATCCC TAAATCCAAA GAGTCTCATT TTGTGTTCAG CCAACAGGGC 
AGTCACCTAG AGAGCATGAG ACTGCCTCTG CTTTCCGACA AGGAGTCGGG CATGATGAAA 
AGCCCAATAT TCACCCCAAC CACGGGCCGC CAGGAGCACG GCCTTCTCAA CATCTACCAT 
GCCATGGAGG GAGCCGAGCA CCTGCACATC CTGGTGGTCA AACAGTATGA AATGCCACTC 
TATAGGAAAT ACTGGCCCAA CCACATCCTG CTGGTGCTGC CCGCCATGTT CAACAACTCA 
GGAGTCGGTG CGGCTCGATT CATGATCAAA GAACTGTCCT ATCACAACCT GGAGCTTGAG 
AGGAACCGAC AAGAGGAGCA AGGCGTCAAA CGGCAGGACG TGTGGCCCTT TATCGTAATG 
ATGGATGACT CCTGCGTCCT GTGGAACGCC CAGCAGCCTG GACCAGACGG CAAAACCGAG 
GTGATGAACG TGTCTCTGAA GAGCGTCTTG CAGCACATGG AAGCCACTCC AAAGATCTCC 
CAGTATGCAG TTTGTGGCCT GCGCAAATGG AGCAGTAGTT TGTCTTCTCA AGCTCCCACA 
AGCCCTTTCT CTCGCTGTCA TCTCCACGAC CTCATCTTGC TGAATGTTGA CCTCACACAG 
AACGTCCAGT ATGACCTCAA TCGGTTCACA TGTGAAGAGG TGGACTTCAA CCTGAGGGCA 
AACAGCAGTG GTCTTCTGCT CTGTCGCTTC AATCAATTCA GCATCATGAA GAAACACATT 
CCCATCGGAG GACATAAAGA CTTCCTCATC AAACCCAAGC TCATGAGAAT AGAGACGCCG 
GTGCGTGTGT GTGCGTCTCA ATATGTGTGC GCTCCGGACA GTGAACAAAC GTTGCTGGCT 
GCGCCAGCTC AGTTCCTGCT GGAGAAGTTT CTGCAGTCCT GTAGCCATCG GCTCTTTCCT 
CTGGCTCTCA GTAACAGTGC TAACCCTGTG TTGTCCATAG ACAGCTACCT CAACCTTGGA 
CCAGAGGTTC AGGTGTGTTA TGTGAGCTCG CGACCTCATT CGGTGAATGT AGATCATCAA 
GGTGTCATTT TCAGTGGCCT GCTGCTCTAC TTGTGTGACT CCTTTGTCGT TTCAAGCCTT 
CTAAAGAAAT TCAACTTCCT CAAAGGCGCC ACGCTGTGTG TGATCTGTCA GGACCGGAGC 
TCTCTGCGTC AGACTATTGT ACGTCTGGAG CTGGAGGATG AGTGGCAATT TCGCCTGCGA 
GATGAGTTTC AAACGGCCAA CTGCAGCGAG GACCGTCCGC TCTACTTCCT CACAGGACGA 
CATATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001349236.1
CDS	336..6164
Translation	MGNSYAGQLKSARFEEALHNSIEASLRSSGGDPQPVFTQLYLEPDQYSGHVEDIKPKMDLSLRSDPSTHVLVKCHSSNSVEDMDDEDDSDTSSPPLPYLQGPPPDGCCTVDGFCQAGKDLRLVSMATESIEVPAGFELVGAKSPSIPEHILVCAVDKRFLPDENGKNALLGFSGNCVGCGEKGFRYFTEFSNHINLKLSTQPKKQKHLKYYLVKNSQGALCKGALICWKDCKTRPFSNSASSSKPSSSSSLSSKENGDTNGHSPSPFPLSDSPPARMQSGSSSGIFGPQELGFLKPLNTPTHGTKTLPIVPTALRVNGLTNGLSMDGRSTLLSPPRTNPLSTPSHGYRTTETGDSPASTAMSTGPPKKRHRSWHPTTLVPIPATAVPVPAIRPLTCSSGPLLSLSNQQPASVSGVIQPQPITAGETVIIPDNLLNSYGVRPVLLIGQGTLPYFFGNVGDLVVSPLLVSCYKGRELNEKTLASLGMSANQLLTTETMILLTLQYLARLGTEQIPLREEFEQIMLKAMLCGPTGPPVSPAQLPWLARMEASVSGGSVQVLVTHGSLGEGISESLRSLSETSPQQQQCLPNYVLIICTSKSGANEFCVLVLGKYQSRALAESMLSTNEFLKEISYELITGKVSVLASHFQSTSLGDNMDKQLVRYQRKRKDRVVQPFQGHLTEYIHSQEAATMIPESGPDLLSDDFQIHPPQLSVARSLLSQVCAIADSGSQSLDLGRFCKVDFLILVPPSHVLVHQTVQRIRQSGVLIDLGIEDVSLAMQKSDKYVVRLDTEVHTKMEAFMRKVKQNPYTLFVLIHDNSHVDLTSALSGSVCHGELQGLADRVVNCPEVLEAINLLVLQVSCFPFTLQSRQSRISTQNEVHWPDTENQQGEASPKDLIYFGLKDYSKSLQWGVASPILRCDDAFERMVKTLLERHPHLHSMVIRSYLLIQQYTEALMALTAAPSLRDHVTPQTLAMVEDLLSVPGRSKHGCGHMLLVRVPSLQLARLAQERLEEARDKLGLQYRFAVLLGSPAAEISLPVHFCARLRAWRGCKNEEWVPHTYEDLEGLPCIVILTGKDPLGETFPRSLKYCDLRLIDSSYLTRTALEQEVGLACSYVTRRVIPKTKTATSREERPREGERSSGETAEHDDLPMELERPPSNASAATRTSGSTTENGVSSSSILDKPSSQSDPCGSRTMMDSCSSPVRFKQECDSQAPSSSSTSSFSSASSSSSSSSSPAAQRPSQSTQAPRECNRTQVFPRTAVLSRAAYTLLAPETLGHPSSASLLPHADVSWSSPLRPPVPHGLGGAEQSLYYRQWTTARQHHADYEGPVPHPHPRRLLLSGPPQVGKTGAYLQFLRILFRMLIRLLEVDVYDEEELEEDVQDKSKVPPSSGPQWPDVEDVRKLRFDLCPHDCKFKYSSPVYANRMPKTQSGVKTERLDTEADPPKRNTVSVRLSLFAAHNAFHHCEQCHHYSEPIPAAQLSDCTFHAFTFCSSMLGEEVQLHFIIPKSKESHFVFSQQGSHLESMRLPLLSDKESGMMKSPIFTPTTGRQEHGLLNIYHAMEGAEHLHILVVKQYEMPLYRKYWPNHILLVLPAMFNNSGVGAARFMIKELSYHNLELERNRQEEQGVKRQDVWPFIVMMDDSCVLWNAQQPGPDGKTEVMNVSLKSVLQHMEATPKISQYAVCGLRKWSSSLSSQAPTSPFSRCHLHDLILLNVDLTQNVQYDLNRFTCEEVDFNLRANSSGLLLCRFNQFSIMKKHIPIGGHKDFLIKPKLMRIETPVRVCASQYVCAPDSEQTLLAAPAQFLLEKFLQSCSHRLFPLALSNSANPVLSIDSYLNLGPEVQVCYVSSRPHSVNVDHQGVIFSGLLLYLCDSFVVSSLLKKFNFLKGATLCVICQDRSSLRQTIVRLELEDEWQFRLRDEFQTANCSEDRPLYFLTGRHI

Target ORF information:

RefSeq Version	NM_001362307.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio GREB1 like retinoic acid receptor coactivator (greb1l), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001362307.1

ORF Insert Sequence:

ATGGGAAATT CATACGCGGG GCAGCTGAAA TCTGCTCGCT TTGAGGAAGC TCTCCATAAC 
TCAATAGAGG CTTCATTAAG GTCAAGCGGT GGTGACCCAC AGCCGGTTTT CACACAGCTA 
TATCTCGAGC CTGATCAGTA TTCTGGCCAT GTAGAAGATA TTAAGCCCAA AATGGACCTA 
AGCCTGAGAT CCGACCCATC CACCCATGTG CTTGTGAAAT GCCACTCTTC AAATTCTGTA 
GAGGATATGG ATGACGAGGA CGATTCAGAC ACCAGCAGCC CACCATTGCC TTACCTGCAG 
GGACCCCCTC CTGATGGCTG CTGCACTGTT GATGGTTTCT GTCAAGCAGG AAAAGATCTG 
CGCCTGGTTT CCATGGCAAC AGAGTCTATC GAGGTTCCTG CAGGGTTTGA GCTGGTCGGT 
GCCAAATCGC CCAGCATCCC AGAGCACATC CTCGTCTGTG CAGTGGACAA GCGCTTCCTG 
CCAGATGAGA ATGGGAAAAA TGCACTTTTA GGGTTTTCAG GGAACTGCGT GGGTTGTGGT 
GAGAAAGGGT TCCGTTACTT CACCGAGTTC TCTAACCACA TTAACCTGAA GTTATCCACT 
CAGCCGAAGA AGCAGAAGCA CTTAAAGTAC TACCTGGTGA AGAACTCTCA GGGTGCTCTG 
TGCAAAGGAG CCCTCATTTG CTGGAAAGAT TGTAAGACAC GACCATTCTC AAACAGTGCT 
TCTTCATCCA AGCCTAGTTC CTCATCCTCA CTAAGCAGCA AAGAAAATGG AGACACAAAT 
GGCCACAGTC CATCTCCCTT CCCCCTCTCA GACTCGCCTC CTGCCCGCAT GCAATCTGGC 
TCATCCTCAG GGATTTTTGG ACCCCAGGAG CTGGGCTTCC TTAAACCATT GAACACACCG 
ACCCACGGGA CCAAGACATT ACCGATCGTT CCCACTGCCC TCCGAGTGAA TGGTCTAACT 
AATGGTCTCA GTATGGATGG ACGCTCCACT CTACTGAGCC CACCTCGTAC AAACCCACTC 
TCCACCCCTA GTCATGGCTA TAGAACCACA GAAACAGGAG ACAGTCCAGC ATCCACTGCT 
ATGTCGACAG GTCCACCAAA AAAGCGGCAT CGTAGTTGGC ATCCCACCAC TTTAGTGCCC 
ATCCCAGCCA CAGCTGTTCC TGTACCAGCC ATCCGACCGC TGACCTGCAG CTCTGGTCCT 
TTATTGTCAC TGTCCAATCA ACAGCCTGCA TCGGTGTCAG GGGTCATACA ACCACAGCCA 
ATCACAGCTG GAGAAACGGT CATCATTCCT GATAACCTTC TGAACTCTTA CGGGGTCCGG 
CCAGTCCTTC TCATTGGTCA AGGCACTTTA CCATATTTCT TTGGCAATGT GGGTGATCTG 
GTGGTGAGCC CCCTGCTGGT GAGCTGCTAC AAGGGCAGAG AGCTCAACGA GAAGACCCTG 
GCCAGCCTGG GCATGTCAGC AAACCAGTTA CTGACCACAG AGACCATGAT ACTGCTCACA 
CTGCAGTACC TCGCTCGACT GGGTACAGAG CAGATACCGT TACGGGAAGA GTTTGAGCAA 
ATCATGTTGA AGGCTATGCT GTGTGGTCCG ACTGGCCCTC CAGTGTCGCC TGCGCAGCTG 
CCCTGGTTAG CCCGAATGGA GGCCAGTGTG TCAGGGGGCA GTGTTCAGGT GCTGGTCACC 
CACGGCTCTC TTGGTGAGGG TATCTCTGAG TCTCTGCGCT CACTGAGTGA AACATCACCA 
CAGCAGCAGC AGTGTCTCCC CAACTATGTG CTCATTATCT GCACCTCCAA AAGTGGAGCC 
AATGAGTTCT GTGTGCTCGT GTTGGGTAAG TATCAGTCTC GAGCCTTGGC AGAGAGCATG 
CTGTCTACAA ACGAGTTTCT GAAGGAGATC AGTTATGAGC TCATCACAGG GAAAGTTAGT 
GTACTAGCCT CACACTTCCA GAGCACCTCT TTAGGTGACA ATATGGACAA ACAGCTGGTT 
CGTTATCAAC GTAAACGCAA AGACAGGGTG GTTCAACCTT TTCAGGGGCA TCTCACCGAG 
TACATCCACT CCCAGGAAGC TGCGACCATG ATACCAGAAT CAGGGCCAGA TCTGCTGAGC 
GATGACTTTC AGATACACCC TCCTCAGTTG AGCGTTGCAC GAAGCCTTTT GTCACAGGTT 
TGTGCTATTG CCGACTCAGG GAGCCAGAGC TTAGATCTGG GCCGATTCTG CAAAGTAGAC 
TTTCTGATTC TGGTGCCGCC CTCCCATGTT CTGGTTCACC AAACAGTCCA GCGAATACGA 
CAATCAGGCG TCCTAATAGA TCTCGGTATA GAGGACGTGT CATTAGCCAT GCAGAAGTCT 
GACAAGTATG TGGTGCGTCT GGACACAGAG GTCCACACAA AAATGGAGGC CTTTATGAGG 
AAGGTCAAGC AGAACCCTTA TACACTCTTC GTCCTCATTC ATGACAACTC GCACGTGGAC 
TTGACAAGTG CTCTTTCAGG GTCGGTTTGT CATGGGGAGC TGCAGGGATT GGCTGATCGC 
GTGGTGAACT GTCCAGAGGT TCTGGAAGCA ATAAACTTAC TGGTGCTGCA GGTCAGCTGC 
TTCCCGTTCA CCCTGCAGAG CCGGCAGTCA CGAATCAGTA CCCAGAATGA AGTGCACTGG 
CCCGACACTG AGAACCAGCA GGGTGAAGCT TCTCCTAAAG ACTTGATCTA CTTTGGGCTG 
AAGGACTACA GCAAGTCTCT GCAGTGGGGA GTGGCGAGTC CAATTCTGCG ATGTGATGAC 
GCCTTTGAGC GGATGGTTAA AACCCTGTTG GAGAGGCACC CACATTTGCA CAGTATGGTG 
ATCCGCAGCT ACCTGTTGAT CCAGCAGTAC ACAGAGGCTC TGATGGCCCT CACAGCGGCC 
CCTTCTTTGC GGGATCACGT GACCCCTCAG ACACTTGCCA TGGTGGAGGA TCTGCTGAGT 
GTGCCAGGCC GCAGCAAGCA TGGGTGCGGA CACATGCTGC TGGTGCGTGT GCCTTCTCTG 
CAGCTGGCAC GACTGGCACA GGAGCGGCTG GAGGAGGCTC GGGACAAGCT GGGGCTGCAG 
TACCGCTTTG CCGTGCTGCT AGGAAGCCCT GCTGCGGAGA TCAGCCTGCC GGTGCACTTC 
TGTGCCCGTC TGCGGGCTTG GAGGGGCTGT AAAAATGAGG AGTGGGTCCC ACATACATAT 
GAAGATCTGG AAGGACTTCC ATGCATTGTC ATCCTCACTG GAAAAGATCC TCTTGGAGAA 
ACCTTCCCGA GGTCTCTAAA GTACTGTGAC TTGCGGCTGA TTGACTCCAG CTACCTGACA 
CGCACAGCGC TGGAACAGGA AGTGGGTTTG GCCTGTTCAT ATGTGACACG TAGAGTAATC 
CCTAAGACCA AAACTGCCAC AAGCAGAGAA GAGAGACCGA GAGAAGGTGA AAGGAGCTCT 
GGAGAAACAG CAGAACACGA TGATCTCCCT ATGGAGCTGG AAAGACCACC CAGCAATGCC 
AGCGCTGCTA CTAGGACCTC CGGCTCCACC ACAGAAAATG GTGTCAGTTC CTCAAGTATA 
CTTGACAAGC CATCCTCGCA GAGTGACCCT TGTGGAAGCA GGACTATGAT GGACTCTTGC 
TCCTCTCCTG TTCGCTTCAA GCAGGAGTGC GACTCTCAGG CTCCCTCATC ATCCAGCACG 
TCCTCATTCT CCTCTGCTTC CTCCTCCTCT TCTTCTTCCT CGTCTCCGGC AGCTCAGAGA 
CCGAGTCAGT CTACACAGGC TCCACGTGAA TGTAACAGGA CACAGGTCTT TCCCCGGACG 
GCGGTGCTGT CTCGGGCTGC GTATACCCTG CTGGCTCCTG AAACTCTGGG TCATCCCAGT 
TCTGCCTCTC TGCTGCCACA TGCGGATGTG TCGTGGAGCA GTCCGCTCAG ACCACCGGTT 
CCTCATGGCC TTGGTGGAGC AGAGCAGTCC CTCTACTATC GTCAGTGGAC CACAGCCAGG 
CAACACCACG CTGATTATGA AGGCCCAGTA CCCCATCCCC ACCCAAGAAG ACTGCTTCTG 
AGCGGCCCAC CACAGGTTGG AAAAACAGGT GCATATCTGC AGTTCCTACG AATTCTGTTC 
CGTATGCTGA TTCGCCTTCT GGAGGTAGAT GTTTATGATG AAGAAGAGCT GGAGGAAGAT 
GTGCAAGACA AATCAAAGGT TCCCCCTTCT AGTGGGCCAC AGTGGCCTGA TGTGGAGGAC 
GTCCGCAAAC TAAGATTTGA CCTCTGCCCT CATGACTGCA AGTTCAAATA TTCAAGTCCA 
GTGTATGCCA ACAGGATGCC AAAAACACAG AGTGGGGTTA AAACCGAAAG ACTGGATACA 
GAAGCGGACC CACCAAAGAG AAACACAGTA TCTGTTAGAC TCAGCCTTTT TGCAGCCCAC 
AATGCATTTC ACCACTGTGA GCAGTGCCAC CATTACAGTG AACCTATTCC TGCTGCTCAG 
CTCTCTGACT GCACCTTCCA CGCTTTCACC TTTTGCTCAT CTATGCTGGG AGAGGAAGTC 
CAGCTGCACT TTATCATCCC TAAATCCAAA GAGTCTCATT TTGTGTTCAG CCAACAGGGC 
AGTCACCTAG AGAGCATGAG ACTGCCTCTG CTTTCCGACA AGGAGTCGGG CATGATGAAA 
AGCCCAATAT TCACCCCAAC CACGGGCCGC CAGGAGCACG GCCTTCTCAA CATCTACCAT 
GCCATGGAGG GAGCCGAGCA CCTGCACATC CTGGTGGTCA AACAGTATGA AATGCCACTC 
TATAGGAAAT ACTGGCCCAA CCACATCCTG CTGGTGCTGC CCGCCATGTT CAACAACTCA 
GGAGTCGGTG CGGCTCGATT CATGATCAAA GAACTGTCCT ATCACAACCT GGAGCTTGAG 
AGGAACCGAC AAGAGGAGCA AGGCGTCAAA CGGCAGGACG TGTGGCCCTT TATCGTAATG 
ATGGATGACT CCTGCGTCCT GTGGAACGCC CAGCAGCCTG GACCAGACGG CAAAACCGAG 
GTGATGAACG TGTCTCTGAA GAGCGTCTTG CAGCACATGG AAGCCACTCC AAAGATCTCC 
CAGTATGCAG TTTGTGGCCT GCGCAAATGG AGCAGTAGTT TGTCTTCTCA AGCTCCCACA 
AGCCCTTTCT CTCGCTGTCA TCTCCACGAC CTCATCTTGC TGAATGTTGA CCTCACACAG 
AACGTCCAGT ATGACCTCAA TCGGTTCACA TGTGAAGAGG TGGACTTCAA CCTGAGGGCA 
AACAGCAGTG GTCTTCTGCT CTGTCGCTTC AATCAATTCA GCATCATGAA GAAACACATT 
CCCATCGGAG GACATAAAGA CTTCCTCATC AAACCCAAGC TCATGAGAAT AGAGACGCCG 
GTGCGTGTGT GTGCGTCTCA ATATGTGTGC GCTCCGGACA GTGAACAAAC GTTGCTGGCT 
GCGCCAGCTC AGTTCCTGCT GGAGAAGTTT CTGCAGTCCT GTAGCCATCG GCTCTTTCCT 
CTGGCTCTCA GTAACAGTGC TAACCCTGTG TTGTCCATAG ACAGCTACCT CAACCTTGGA 
CCAGAGGTTC AGGTGTGTTA TGTGAGCTCG CGACCTCATT CGGTGAATGT AGATCATCAA 
GGTGTCATTT TCAGTGGCCT GCTGCTCTAC TTGTGTGACT CCTTTGTCGT TTCAAGCCTT 
CTAAAGAAAT TCAACTTCCT CAAAGGCGCC ACGCTGTGTG TGATCTGTCA GGACCGGAGC 
TCTCTGCGTC AGACTATTGT ACGTCTGGAG CTGGAGGATG AGTGGCAATT TCGCCTGCGA 
GATGAGTTTC AAACGGCCAA CTGCAGCGAG GACCGTCCGC TCTACTTCCT CACAGGACGA 
CATATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GREB1L ( XM_001344416.8 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_001344452.4 Danio rerio growth regulation by estrogen in breast cancer-like (greb1l), transcript variant X1, mRNA.

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CloneID	ODa06787
Clone ID Related Accession (Same CDS sequence)	XM_001344416.8 , XM_009298040.1 , XM_009298040.2 , XM_021466912.1 , NM_001362307.1 , XM_001344416.7
Accession Version	XM_001344416.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	GREB1-like protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001344416.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAATT CATACGCGGG GCAGCTGAAA TCTGCTCGCT TTGAGGAAGC TCTCCATAAC 
TCAATAGAGG CTTCATTAAG GTCAAGCGGT GGTGACCCAC AGCCGGTTTT CACACAGCTA 
TATCTCGAGC CTGATCAGTA TTCTGGCCAT GTAGAAGATA TTAAGCCCAA AATGGACCTA 
AGCCTGAGAT CCGACCCATC CACCCATGTG CTTGTGAAAT GCCACTCTTC AAATTCTGTA 
GAGGATATGG ATGACGAGGA CGATTCAGAC ACCAGCAGCC CACCATTGCC TTACCTGCAG 
GGACCCCCTC CTGATGGCTG CTGCACTGTT GATGGTTTCT GTCAAGCAGG AAAAGATCTG 
CGCCTGGTTT CCATGGCAAC AGAGTCTATC GAGGTTCCTG CAGGGTTTGA GCTGGTCGGT 
GCCAAATCGC CCAGCATCCC AGAGCACATC CTCGTCTGTG CAGTGGACAA GCGCTTCCTG 
CCAGATGAGA ATGGGAAAAA TGCACTTTTA GGGTTTTCAG GGAACTGCGT GGGTTGTGGT 
GAGAAAGGGT TCCGTTACTT CACCGAGTTC TCTAACCACA TTAACCTGAA GTTATCCACT 
CAGCCGAAGA AGCAGAAGCA CTTAAAGTAC TACCTGGTGA AGAACTCTCA GGGTGCTCTG 
TGCAAAGGAG CCCTCATTTG CTGGAAAGAT TGTAAGACAC GACCATTCTC AAACAGTGCT 
TCTTCATCCA AGCCTAGTTC CTCATCCTCA CTAAGCAGCA AAGAAAATGG AGACACAAAT 
GGCCACAGTC CATCTCCCTT CCCCCTCTCA GACTCGCCTC CTGCCCGCAT GCAATCTGGC 
TCATCCTCAG GGATTTTTGG ACCCCAGGAG CTGGGCTTCC TTAAACCATT GAACACACCG 
ACCCACGGGA CCAAGACATT ACCGATCGTT CCCACTGCCC TCCGAGTGAA TGGTCTAACT 
AATGGTCTCA GTATGGATGG ACGCTCCACT CTACTGAGCC CACCTCGTAC AAACCCACTC 
TCCACCCCTA GTCATGGCTA TAGAACCACA GAAACAGGAG ACAGTCCAGC ATCCACTGCT 
ATGTCGACAG GTCCACCAAA AAAGCGGCAT CGTAGTTGGC ATCCCACCAC TTTAGTGCCC 
ATCCCAGCCA CAGCTGTTCC TGTACCAGCC ATCCGACCGC TGACCTGCAG CTCTGGTCCT 
TTATTGTCAC TGTCCAATCA ACAGCCTGCA TCGGTGTCAG GGGTCATACA ACCACAGCCA 
ATCACAGCTG GAGAAACGGT CATCATTCCT GATAACCTTC TGAACTCTTA CGGGGTCCGG 
CCAGTCCTTC TCATTGGTCA AGGCACTTTA CCATATTTCT TTGGCAATGT GGGTGATCTG 
GTGGTGAGCC CCCTGCTGGT GAGCTGCTAC AAGGGCAGAG AGCTCAACGA GAAGACCCTG 
GCCAGCCTGG GCATGTCAGC AAACCAGTTA CTGACCACAG AGACCATGAT ACTGCTCACA 
CTGCAGTACC TCGCTCGACT GGGTACAGAG CAGATACCGT TACGGGAAGA GTTTGAGCAA 
ATCATGTTGA AGGCTATGCT GTGTGGTCCG ACTGGCCCTC CAGTGTCGCC TGCGCAGCTG 
CCCTGGTTAG CCCGAATGGA GGCCAGTGTG TCAGGGGGCA GTGTTCAGGT GCTGGTCACC 
CACGGCTCTC TTGGTGAGGG TATCTCTGAG TCTCTGCGCT CACTGAGTGA AACATCACCA 
CAGCAGCAGC AGTGTCTCCC CAACTATGTG CTCATTATCT GCACCTCCAA AAGTGGAGCC 
AATGAGTTCT GTGTGCTCGT GTTGGGTAAG TATCAGTCTC GAGCCTTGGC AGAGAGCATG 
CTGTCTACAA ACGAGTTTCT GAAGGAGATC AGTTATGAGC TCATCACAGG GAAAGTTAGT 
GTACTAGCCT CACACTTCCA GAGCACCTCT TTAGGTGACA ATATGGACAA ACAGCTGGTT 
CGTTATCAAC GTAAACGCAA AGACAGGGTG GTTCAACCTT TTCAGGGGCA TCTCACCGAG 
TACATCCACT CCCAGGAAGC TGCGACCATG ATACCAGAAT CAGGGCCAGA TCTGCTGAGC 
GATGACTTTC AGATACACCC TCCTCAGTTG AGCGTTGCAC GAAGCCTTTT GTCACAGGTT 
TGTGCTATTG CCGACTCAGG GAGCCAGAGC TTAGATCTGG GCCGATTCTG CAAAGTAGAC 
TTTCTGATTC TGGTGCCGCC CTCCCATGTT CTGGTTCACC AAACAGTCCA GCGAATACGA 
CAATCAGGCG TCCTAATAGA TCTCGGTATA GAGGACGTGT CATTAGCCAT GCAGAAGTCT 
GACAAGTATG TGGTGCGTCT GGACACAGAG GTCCACACAA AAATGGAGGC CTTTATGAGG 
AAGGTCAAGC AGAACCCTTA TACACTCTTC GTCCTCATTC ATGACAACTC GCACGTGGAC 
TTGACAAGTG CTCTTTCAGG GTCGGTTTGT CATGGGGAGC TGCAGGGATT GGCTGATCGC 
GTGGTGAACT GTCCAGAGGT TCTGGAAGCA ATAAACTTAC TGGTGCTGCA GGTCAGCTGC 
TTCCCGTTCA CCCTGCAGAG CCGGCAGTCA CGAATCAGTA CCCAGAATGA AGTGCACTGG 
CCCGACACTG AGAACCAGCA GGGTGAAGCT TCTCCTAAAG ACTTGATCTA CTTTGGGCTG 
AAGGACTACA GCAAGTCTCT GCAGTGGGGA GTGGCGAGTC CAATTCTGCG ATGTGATGAC 
GCCTTTGAGC GGATGGTTAA AACCCTGTTG GAGAGGCACC CACATTTGCA CAGTATGGTG 
ATCCGCAGCT ACCTGTTGAT CCAGCAGTAC ACAGAGGCTC TGATGGCCCT CACAGCGGCC 
CCTTCTTTGC GGGATCACGT GACCCCTCAG ACACTTGCCA TGGTGGAGGA TCTGCTGAGT 
GTGCCAGGCC GCAGCAAGCA TGGGTGCGGA CACATGCTGC TGGTGCGTGT GCCTTCTCTG 
CAGCTGGCAC GACTGGCACA GGAGCGGCTG GAGGAGGCTC GGGACAAGCT GGGGCTGCAG 
TACCGCTTTG CCGTGCTGCT AGGAAGCCCT GCTGCGGAGA TCAGCCTGCC GGTGCACTTC 
TGTGCCCGTC TGCGGGCTTG GAGGGGCTGT AAAAATGAGG AGTGGGTCCC ACATACATAT 
GAAGATCTGG AAGGACTTCC ATGCATTGTC ATCCTCACTG GAAAAGATCC TCTTGGAGAA 
ACCTTCCCGA GGTCTCTAAA GTACTGTGAC TTGCGGCTGA TTGACTCCAG CTACCTGACA 
CGCACAGCGC TGGAACAGGA AGTGGGTTTG GCCTGTTCAT ATGTGACACG TAGAGTAATC 
CCTAAGACCA AAACTGCCAC AAGCAGAGAA GAGAGACCGA GAGAAGGTGA AAGGAGCTCT 
GGAGAAACAG CAGAACACGA TGATCTCCCT ATGGAGCTGG AAAGACCACC CAGCAATGCC 
AGCGCTGCTA CTAGGACCTC CGGCTCCACC ACAGAAAATG GTGTCAGTTC CTCAAGTATA 
CTTGACAAGC CATCCTCGCA GAGTGACCCT TGTGGAAGCA GGACTATGAT GGACTCTTGC 
TCCTCTCCTG TTCGCTTCAA GCAGGAGTGC GACTCTCAGG CTCCCTCATC ATCCAGCACG 
TCCTCATTCT CCTCTGCTTC CTCCTCCTCT TCTTCTTCCT CGTCTCCGGC AGCTCAGAGA 
CCGAGTCAGT CTACACAGGC TCCACGTGAA TGTAACAGGA CACAGGTCTT TCCCCGGACG 
GCGGTGCTGT CTCGGGCTGC GTATACCCTG CTGGCTCCTG AAACTCTGGG TCATCCCAGT 
TCTGCCTCTC TGCTGCCACA TGCGGATGTG TCGTGGAGCA GTCCGCTCAG ACCACCGGTT 
CCTCATGGCC TTGGTGGAGC AGAGCAGTCC CTCTACTATC GTCAGTGGAC CACAGCCAGG 
CAACACCACG CTGATTATGA AGGCCCAGTA CCCCATCCCC ACCCAAGAAG ACTGCTTCTG 
AGCGGCCCAC CACAGGTTGG AAAAACAGGT GCATATCTGC AGTTCCTACG AATTCTGTTC 
CGTATGCTGA TTCGCCTTCT GGAGGTAGAT GTTTATGATG AAGAAGAGCT GGAGGAAGAT 
GTGCAAGACA AATCAAAGGT TCCCCCTTCT AGTGGGCCAC AGTGGCCTGA TGTGGAGGAC 
GTCCGCAAAC TAAGATTTGA CCTCTGCCCT CATGACTGCA AGTTCAAATA TTCAAGTCCA 
GTGTATGCCA ACAGGATGCC AAAAACACAG AGTGGGGTTA AAACCGAAAG ACTGGATACA 
GAAGCGGACC CACCAAAGAG AAACACAGTA TCTGTTAGAC TCAGCCTTTT TGCAGCCCAC 
AATGCATTTC ACCACTGTGA GCAGTGCCAC CATTACAGTG AACCTATTCC TGCTGCTCAG 
CTCTCTGACT GCACCTTCCA CGCTTTCACC TTTTGCTCAT CTATGCTGGG AGAGGAAGTC 
CAGCTGCACT TTATCATCCC TAAATCCAAA GAGTCTCATT TTGTGTTCAG CCAACAGGGC 
AGTCACCTAG AGAGCATGAG ACTGCCTCTG CTTTCCGACA AGGAGTCGGG CATGATGAAA 
AGCCCAATAT TCACCCCAAC CACGGGCCGC CAGGAGCACG GCCTTCTCAA CATCTACCAT 
GCCATGGAGG GAGCCGAGCA CCTGCACATC CTGGTGGTCA AACAGTATGA AATGCCACTC 
TATAGGAAAT ACTGGCCCAA CCACATCCTG CTGGTGCTGC CCGCCATGTT CAACAACTCA 
GGAGTCGGTG CGGCTCGATT CATGATCAAA GAACTGTCCT ATCACAACCT GGAGCTTGAG 
AGGAACCGAC AAGAGGAGCA AGGCGTCAAA CGGCAGGACG TGTGGCCCTT TATCGTAATG 
ATGGATGACT CCTGCGTCCT GTGGAACGCC CAGCAGCCTG GACCAGACGG CAAAACCGAG 
GTGATGAACG TGTCTCTGAA GAGCGTCTTG CAGCACATGG AAGCCACTCC AAAGATCTCC 
CAGTATGCAG TTTGTGGCCT GCGCAAATGG AGCAGTAGTT TGTCTTCTCA AGCTCCCACA 
AGCCCTTTCT CTCGCTGTCA TCTCCACGAC CTCATCTTGC TGAATGTTGA CCTCACACAG 
AACGTCCAGT ATGACCTCAA TCGGTTCACA TGTGAAGAGG TGGACTTCAA CCTGAGGGCA 
AACAGCAGTG GTCTTCTGCT CTGTCGCTTC AATCAATTCA GCATCATGAA GAAACACATT 
CCCATCGGAG GACATAAAGA CTTCCTCATC AAACCCAAGC TCATGAGAAT AGAGACGCCG 
GTGCGTGTGT GTGCGTCTCA ATATGTGTGC GCTCCGGACA GTGAACAAAC GTTGCTGGCT 
GCGCCAGCTC AGTTCCTGCT GGAGAAGTTT CTGCAGTCCT GTAGCCATCG GCTCTTTCCT 
CTGGCTCTCA GTAACAGTGC TAACCCTGTG TTGTCCATAG ACAGCTACCT CAACCTTGGA 
CCAGAGGTTC AGGTGTGTTA TGTGAGCTCG CGACCTCATT CGGTGAATGT AGATCATCAA 
GGTGTCATTT TCAGTGGCCT GCTGCTCTAC TTGTGTGACT CCTTTGTCGT TTCAAGCCTT 
CTAAAGAAAT TCAACTTCCT CAAAGGCGCC ACGCTGTGTG TGATCTGTCA GGACCGGAGC 
TCTCTGCGTC AGACTATTGT ACGTCTGGAG CTGGAGGATG AGTGGCAATT TCGCCTGCGA 
GATGAGTTTC AAACGGCCAA CTGCAGCGAG GACCGTCCGC TCTACTTCCT CACAGGACGA 
CATATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001344452.4
CDS	373..6201
Translation	MGNSYAGQLKSARFEEALHNSIEASLRSSGGDPQPVFTQLYLEPDQYSGHVEDIKPKMDLSLRSDPSTHVLVKCHSSNSVEDMDDEDDSDTSSPPLPYLQGPPPDGCCTVDGFCQAGKDLRLVSMATESIEVPAGFELVGAKSPSIPEHILVCAVDKRFLPDENGKNALLGFSGNCVGCGEKGFRYFTEFSNHINLKLSTQPKKQKHLKYYLVKNSQGALCKGALICWKDCKTRPFSNSASSSKPSSSSSLSSKENGDTNGHSPSPFPLSDSPPARMQSGSSSGIFGPQELGFLKPLNTPTHGTKTLPIVPTALRVNGLTNGLSMDGRSTLLSPPRTNPLSTPSHGYRTTETGDSPASTAMSTGPPKKRHRSWHPTTLVPIPATAVPVPAIRPLTCSSGPLLSLSNQQPASVSGVIQPQPITAGETVIIPDNLLNSYGVRPVLLIGQGTLPYFFGNVGDLVVSPLLVSCYKGRELNEKTLASLGMSANQLLTTETMILLTLQYLARLGTEQIPLREEFEQIMLKAMLCGPTGPPVSPAQLPWLARMEASVSGGSVQVLVTHGSLGEGISESLRSLSETSPQQQQCLPNYVLIICTSKSGANEFCVLVLGKYQSRALAESMLSTNEFLKEISYELITGKVSVLASHFQSTSLGDNMDKQLVRYQRKRKDRVVQPFQGHLTEYIHSQEAATMIPESGPDLLSDDFQIHPPQLSVARSLLSQVCAIADSGSQSLDLGRFCKVDFLILVPPSHVLVHQTVQRIRQSGVLIDLGIEDVSLAMQKSDKYVVRLDTEVHTKMEAFMRKVKQNPYTLFVLIHDNSHVDLTSALSGSVCHGELQGLADRVVNCPEVLEAINLLVLQVSCFPFTLQSRQSRISTQNEVHWPDTENQQGEASPKDLIYFGLKDYSKSLQWGVASPILRCDDAFERMVKTLLERHPHLHSMVIRSYLLIQQYTEALMALTAAPSLRDHVTPQTLAMVEDLLSVPGRSKHGCGHMLLVRVPSLQLARLAQERLEEARDKLGLQYRFAVLLGSPAAEISLPVHFCARLRAWRGCKNEEWVPHTYEDLEGLPCIVILTGKDPLGETFPRSLKYCDLRLIDSSYLTRTALEQEVGLACSYVTRRVIPKTKTATSREERPREGERSSGETAEHDDLPMELERPPSNASAATRTSGSTTENGVSSSSILDKPSSQSDPCGSRTMMDSCSSPVRFKQECDSQAPSSSSTSSFSSASSSSSSSSSPAAQRPSQSTQAPRECNRTQVFPRTAVLSRAAYTLLAPETLGHPSSASLLPHADVSWSSPLRPPVPHGLGGAEQSLYYRQWTTARQHHADYEGPVPHPHPRRLLLSGPPQVGKTGAYLQFLRILFRMLIRLLEVDVYDEEELEEDVQDKSKVPPSSGPQWPDVEDVRKLRFDLCPHDCKFKYSSPVYANRMPKTQSGVKTERLDTEADPPKRNTVSVRLSLFAAHNAFHHCEQCHHYSEPIPAAQLSDCTFHAFTFCSSMLGEEVQLHFIIPKSKESHFVFSQQGSHLESMRLPLLSDKESGMMKSPIFTPTTGRQEHGLLNIYHAMEGAEHLHILVVKQYEMPLYRKYWPNHILLVLPAMFNNSGVGAARFMIKELSYHNLELERNRQEEQGVKRQDVWPFIVMMDDSCVLWNAQQPGPDGKTEVMNVSLKSVLQHMEATPKISQYAVCGLRKWSSSLSSQAPTSPFSRCHLHDLILLNVDLTQNVQYDLNRFTCEEVDFNLRANSSGLLLCRFNQFSIMKKHIPIGGHKDFLIKPKLMRIETPVRVCASQYVCAPDSEQTLLAAPAQFLLEKFLQSCSHRLFPLALSNSANPVLSIDSYLNLGPEVQVCYVSSRPHSVNVDHQGVIFSGLLLYLCDSFVVSSLLKKFNFLKGATLCVICQDRSSLRQTIVRLELEDEWQFRLRDEFQTANCSEDRPLYFLTGRHI

Target ORF information:

RefSeq Version	XM_001344416.8
Organism	Danio rerio(zebrafish)
Definition	Danio rerio growth regulation by estrogen in breast cancer-like (greb1l), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001344416.8

ORF Insert Sequence:

ATGGGAAATT CATACGCGGG GCAGCTGAAA TCTGCTCGCT TTGAGGAAGC TCTCCATAAC 
TCAATAGAGG CTTCATTAAG GTCAAGCGGT GGTGACCCAC AGCCGGTTTT CACACAGCTA 
TATCTCGAGC CTGATCAGTA TTCTGGCCAT GTAGAAGATA TTAAGCCCAA AATGGACCTA 
AGCCTGAGAT CCGACCCATC CACCCATGTG CTTGTGAAAT GCCACTCTTC AAATTCTGTA 
GAGGATATGG ATGACGAGGA CGATTCAGAC ACCAGCAGCC CACCATTGCC TTACCTGCAG 
GGACCCCCTC CTGATGGCTG CTGCACTGTT GATGGTTTCT GTCAAGCAGG AAAAGATCTG 
CGCCTGGTTT CCATGGCAAC AGAGTCTATC GAGGTTCCTG CAGGGTTTGA GCTGGTCGGT 
GCCAAATCGC CCAGCATCCC AGAGCACATC CTCGTCTGTG CAGTGGACAA GCGCTTCCTG 
CCAGATGAGA ATGGGAAAAA TGCACTTTTA GGGTTTTCAG GGAACTGCGT GGGTTGTGGT 
GAGAAAGGGT TCCGTTACTT CACCGAGTTC TCTAACCACA TTAACCTGAA GTTATCCACT 
CAGCCGAAGA AGCAGAAGCA CTTAAAGTAC TACCTGGTGA AGAACTCTCA GGGTGCTCTG 
TGCAAAGGAG CCCTCATTTG CTGGAAAGAT TGTAAGACAC GACCATTCTC AAACAGTGCT 
TCTTCATCCA AGCCTAGTTC CTCATCCTCA CTAAGCAGCA AAGAAAATGG AGACACAAAT 
GGCCACAGTC CATCTCCCTT CCCCCTCTCA GACTCGCCTC CTGCCCGCAT GCAATCTGGC 
TCATCCTCAG GGATTTTTGG ACCCCAGGAG CTGGGCTTCC TTAAACCATT GAACACACCG 
ACCCACGGGA CCAAGACATT ACCGATCGTT CCCACTGCCC TCCGAGTGAA TGGTCTAACT 
AATGGTCTCA GTATGGATGG ACGCTCCACT CTACTGAGCC CACCTCGTAC AAACCCACTC 
TCCACCCCTA GTCATGGCTA TAGAACCACA GAAACAGGAG ACAGTCCAGC ATCCACTGCT 
ATGTCGACAG GTCCACCAAA AAAGCGGCAT CGTAGTTGGC ATCCCACCAC TTTAGTGCCC 
ATCCCAGCCA CAGCTGTTCC TGTACCAGCC ATCCGACCGC TGACCTGCAG CTCTGGTCCT 
TTATTGTCAC TGTCCAATCA ACAGCCTGCA TCGGTGTCAG GGGTCATACA ACCACAGCCA 
ATCACAGCTG GAGAAACGGT CATCATTCCT GATAACCTTC TGAACTCTTA CGGGGTCCGG 
CCAGTCCTTC TCATTGGTCA AGGCACTTTA CCATATTTCT TTGGCAATGT GGGTGATCTG 
GTGGTGAGCC CCCTGCTGGT GAGCTGCTAC AAGGGCAGAG AGCTCAACGA GAAGACCCTG 
GCCAGCCTGG GCATGTCAGC AAACCAGTTA CTGACCACAG AGACCATGAT ACTGCTCACA 
CTGCAGTACC TCGCTCGACT GGGTACAGAG CAGATACCGT TACGGGAAGA GTTTGAGCAA 
ATCATGTTGA AGGCTATGCT GTGTGGTCCG ACTGGCCCTC CAGTGTCGCC TGCGCAGCTG 
CCCTGGTTAG CCCGAATGGA GGCCAGTGTG TCAGGGGGCA GTGTTCAGGT GCTGGTCACC 
CACGGCTCTC TTGGTGAGGG TATCTCTGAG TCTCTGCGCT CACTGAGTGA AACATCACCA 
CAGCAGCAGC AGTGTCTCCC CAACTATGTG CTCATTATCT GCACCTCCAA AAGTGGAGCC 
AATGAGTTCT GTGTGCTCGT GTTGGGTAAG TATCAGTCTC GAGCCTTGGC AGAGAGCATG 
CTGTCTACAA ACGAGTTTCT GAAGGAGATC AGTTATGAGC TCATCACAGG GAAAGTTAGT 
GTACTAGCCT CACACTTCCA GAGCACCTCT TTAGGTGACA ATATGGACAA ACAGCTGGTT 
CGTTATCAAC GTAAACGCAA AGACAGGGTG GTTCAACCTT TTCAGGGGCA TCTCACCGAG 
TACATCCACT CCCAGGAAGC TGCGACCATG ATACCAGAAT CAGGGCCAGA TCTGCTGAGC 
GATGACTTTC AGATACACCC TCCTCAGTTG AGCGTTGCAC GAAGCCTTTT GTCACAGGTT 
TGTGCTATTG CCGACTCAGG GAGCCAGAGC TTAGATCTGG GCCGATTCTG CAAAGTAGAC 
TTTCTGATTC TGGTGCCGCC CTCCCATGTT CTGGTTCACC AAACAGTCCA GCGAATACGA 
CAATCAGGCG TCCTAATAGA TCTCGGTATA GAGGACGTGT CATTAGCCAT GCAGAAGTCT 
GACAAGTATG TGGTGCGTCT GGACACAGAG GTCCACACAA AAATGGAGGC CTTTATGAGG 
AAGGTCAAGC AGAACCCTTA TACACTCTTC GTCCTCATTC ATGACAACTC GCACGTGGAC 
TTGACAAGTG CTCTTTCAGG GTCGGTTTGT CATGGGGAGC TGCAGGGATT GGCTGATCGC 
GTGGTGAACT GTCCAGAGGT TCTGGAAGCA ATAAACTTAC TGGTGCTGCA GGTCAGCTGC 
TTCCCGTTCA CCCTGCAGAG CCGGCAGTCA CGAATCAGTA CCCAGAATGA AGTGCACTGG 
CCCGACACTG AGAACCAGCA GGGTGAAGCT TCTCCTAAAG ACTTGATCTA CTTTGGGCTG 
AAGGACTACA GCAAGTCTCT GCAGTGGGGA GTGGCGAGTC CAATTCTGCG ATGTGATGAC 
GCCTTTGAGC GGATGGTTAA AACCCTGTTG GAGAGGCACC CACATTTGCA CAGTATGGTG 
ATCCGCAGCT ACCTGTTGAT CCAGCAGTAC ACAGAGGCTC TGATGGCCCT CACAGCGGCC 
CCTTCTTTGC GGGATCACGT GACCCCTCAG ACACTTGCCA TGGTGGAGGA TCTGCTGAGT 
GTGCCAGGCC GCAGCAAGCA TGGGTGCGGA CACATGCTGC TGGTGCGTGT GCCTTCTCTG 
CAGCTGGCAC GACTGGCACA GGAGCGGCTG GAGGAGGCTC GGGACAAGCT GGGGCTGCAG 
TACCGCTTTG CCGTGCTGCT AGGAAGCCCT GCTGCGGAGA TCAGCCTGCC GGTGCACTTC 
TGTGCCCGTC TGCGGGCTTG GAGGGGCTGT AAAAATGAGG AGTGGGTCCC ACATACATAT 
GAAGATCTGG AAGGACTTCC ATGCATTGTC ATCCTCACTG GAAAAGATCC TCTTGGAGAA 
ACCTTCCCGA GGTCTCTAAA GTACTGTGAC TTGCGGCTGA TTGACTCCAG CTACCTGACA 
CGCACAGCGC TGGAACAGGA AGTGGGTTTG GCCTGTTCAT ATGTGACACG TAGAGTAATC 
CCTAAGACCA AAACTGCCAC AAGCAGAGAA GAGAGACCGA GAGAAGGTGA AAGGAGCTCT 
GGAGAAACAG CAGAACACGA TGATCTCCCT ATGGAGCTGG AAAGACCACC CAGCAATGCC 
AGCGCTGCTA CTAGGACCTC CGGCTCCACC ACAGAAAATG GTGTCAGTTC CTCAAGTATA 
CTTGACAAGC CATCCTCGCA GAGTGACCCT TGTGGAAGCA GGACTATGAT GGACTCTTGC 
TCCTCTCCTG TTCGCTTCAA GCAGGAGTGC GACTCTCAGG CTCCCTCATC ATCCAGCACG 
TCCTCATTCT CCTCTGCTTC CTCCTCCTCT TCTTCTTCCT CGTCTCCGGC AGCTCAGAGA 
CCGAGTCAGT CTACACAGGC TCCACGTGAA TGTAACAGGA CACAGGTCTT TCCCCGGACG 
GCGGTGCTGT CTCGGGCTGC GTATACCCTG CTGGCTCCTG AAACTCTGGG TCATCCCAGT 
TCTGCCTCTC TGCTGCCACA TGCGGATGTG TCGTGGAGCA GTCCGCTCAG ACCACCGGTT 
CCTCATGGCC TTGGTGGAGC AGAGCAGTCC CTCTACTATC GTCAGTGGAC CACAGCCAGG 
CAACACCACG CTGATTATGA AGGCCCAGTA CCCCATCCCC ACCCAAGAAG ACTGCTTCTG 
AGCGGCCCAC CACAGGTTGG AAAAACAGGT GCATATCTGC AGTTCCTACG AATTCTGTTC 
CGTATGCTGA TTCGCCTTCT GGAGGTAGAT GTTTATGATG AAGAAGAGCT GGAGGAAGAT 
GTGCAAGACA AATCAAAGGT TCCCCCTTCT AGTGGGCCAC AGTGGCCTGA TGTGGAGGAC 
GTCCGCAAAC TAAGATTTGA CCTCTGCCCT CATGACTGCA AGTTCAAATA TTCAAGTCCA 
GTGTATGCCA ACAGGATGCC AAAAACACAG AGTGGGGTTA AAACCGAAAG ACTGGATACA 
GAAGCGGACC CACCAAAGAG AAACACAGTA TCTGTTAGAC TCAGCCTTTT TGCAGCCCAC 
AATGCATTTC ACCACTGTGA GCAGTGCCAC CATTACAGTG AACCTATTCC TGCTGCTCAG 
CTCTCTGACT GCACCTTCCA CGCTTTCACC TTTTGCTCAT CTATGCTGGG AGAGGAAGTC 
CAGCTGCACT TTATCATCCC TAAATCCAAA GAGTCTCATT TTGTGTTCAG CCAACAGGGC 
AGTCACCTAG AGAGCATGAG ACTGCCTCTG CTTTCCGACA AGGAGTCGGG CATGATGAAA 
AGCCCAATAT TCACCCCAAC CACGGGCCGC CAGGAGCACG GCCTTCTCAA CATCTACCAT 
GCCATGGAGG GAGCCGAGCA CCTGCACATC CTGGTGGTCA AACAGTATGA AATGCCACTC 
TATAGGAAAT ACTGGCCCAA CCACATCCTG CTGGTGCTGC CCGCCATGTT CAACAACTCA 
GGAGTCGGTG CGGCTCGATT CATGATCAAA GAACTGTCCT ATCACAACCT GGAGCTTGAG 
AGGAACCGAC AAGAGGAGCA AGGCGTCAAA CGGCAGGACG TGTGGCCCTT TATCGTAATG 
ATGGATGACT CCTGCGTCCT GTGGAACGCC CAGCAGCCTG GACCAGACGG CAAAACCGAG 
GTGATGAACG TGTCTCTGAA GAGCGTCTTG CAGCACATGG AAGCCACTCC AAAGATCTCC 
CAGTATGCAG TTTGTGGCCT GCGCAAATGG AGCAGTAGTT TGTCTTCTCA AGCTCCCACA 
AGCCCTTTCT CTCGCTGTCA TCTCCACGAC CTCATCTTGC TGAATGTTGA CCTCACACAG 
AACGTCCAGT ATGACCTCAA TCGGTTCACA TGTGAAGAGG TGGACTTCAA CCTGAGGGCA 
AACAGCAGTG GTCTTCTGCT CTGTCGCTTC AATCAATTCA GCATCATGAA GAAACACATT 
CCCATCGGAG GACATAAAGA CTTCCTCATC AAACCCAAGC TCATGAGAAT AGAGACGCCG 
GTGCGTGTGT GTGCGTCTCA ATATGTGTGC GCTCCGGACA GTGAACAAAC GTTGCTGGCT 
GCGCCAGCTC AGTTCCTGCT GGAGAAGTTT CTGCAGTCCT GTAGCCATCG GCTCTTTCCT 
CTGGCTCTCA GTAACAGTGC TAACCCTGTG TTGTCCATAG ACAGCTACCT CAACCTTGGA 
CCAGAGGTTC AGGTGTGTTA TGTGAGCTCG CGACCTCATT CGGTGAATGT AGATCATCAA 
GGTGTCATTT TCAGTGGCCT GCTGCTCTAC TTGTGTGACT CCTTTGTCGT TTCAAGCCTT 
CTAAAGAAAT TCAACTTCCT CAAAGGCGCC ACGCTGTGTG TGATCTGTCA GGACCGGAGC 
TCTCTGCGTC AGACTATTGT ACGTCTGGAG CTGGAGGATG AGTGGCAATT TCGCCTGCGA 
GATGAGTTTC AAACGGCCAA CTGCAGCGAG GACCGTCCGC TCTACTTCCT CACAGGACGA 
CATATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GREB1L ( XM_009298040.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009296315.1 Danio rerio growth regulation by estrogen in breast cancer-like (greb1l), transcript variant X2, mRNA.

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CloneID	ODa06787
Clone ID Related Accession (Same CDS sequence)	XM_001344416.8 , XM_009298040.1 , XM_009298040.2 , XM_021466912.1 , NM_001362307.1 , XM_001344416.7
Accession Version	XM_009298040.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	GREB1-like protein
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAATT CATACGCGGG GCAGCTGAAA TCTGCTCGCT TTGAGGAAGC TCTCCATAAC 
TCAATAGAGG CTTCATTAAG GTCAAGCGGT GGTGACCCAC AGCCGGTTTT CACACAGCTA 
TATCTCGAGC CTGATCAGTA TTCTGGCCAT GTAGAAGATA TTAAGCCCAA AATGGACCTA 
AGCCTGAGAT CCGACCCATC CACCCATGTG CTTGTGAAAT GCCACTCTTC AAATTCTGTA 
GAGGATATGG ATGACGAGGA CGATTCAGAC ACCAGCAGCC CACCATTGCC TTACCTGCAG 
GGACCCCCTC CTGATGGCTG CTGCACTGTT GATGGTTTCT GTCAAGCAGG AAAAGATCTG 
CGCCTGGTTT CCATGGCAAC AGAGTCTATC GAGGTTCCTG CAGGGTTTGA GCTGGTCGGT 
GCCAAATCGC CCAGCATCCC AGAGCACATC CTCGTCTGTG CAGTGGACAA GCGCTTCCTG 
CCAGATGAGA ATGGGAAAAA TGCACTTTTA GGGTTTTCAG GGAACTGCGT GGGTTGTGGT 
GAGAAAGGGT TCCGTTACTT CACCGAGTTC TCTAACCACA TTAACCTGAA GTTATCCACT 
CAGCCGAAGA AGCAGAAGCA CTTAAAGTAC TACCTGGTGA AGAACTCTCA GGGTGCTCTG 
TGCAAAGGAG CCCTCATTTG CTGGAAAGAT TGTAAGACAC GACCATTCTC AAACAGTGCT 
TCTTCATCCA AGCCTAGTTC CTCATCCTCA CTAAGCAGCA AAGAAAATGG AGACACAAAT 
GGCCACAGTC CATCTCCCTT CCCCCTCTCA GACTCGCCTC CTGCCCGCAT GCAATCTGGC 
TCATCCTCAG GGATTTTTGG ACCCCAGGAG CTGGGCTTCC TTAAACCATT GAACACACCG 
ACCCACGGGA CCAAGACATT ACCGATCGTT CCCACTGCCC TCCGAGTGAA TGGTCTAACT 
AATGGTCTCA GTATGGATGG ACGCTCCACT CTACTGAGCC CACCTCGTAC AAACCCACTC 
TCCACCCCTA GTCATGGCTA TAGAACCACA GAAACAGGAG ACAGTCCAGC ATCCACTGCT 
ATGTCGACAG GTCCACCAAA AAAGCGGCAT CGTAGTTGGC ATCCCACCAC TTTAGTGCCC 
ATCCCAGCCA CAGCTGTTCC TGTACCAGCC ATCCGACCGC TGACCTGCAG CTCTGGTCCT 
TTATTGTCAC TGTCCAATCA ACAGCCTGCA TCGGTGTCAG GGGTCATACA ACCACAGCCA 
ATCACAGCTG GAGAAACGGT CATCATTCCT GATAACCTTC TGAACTCTTA CGGGGTCCGG 
CCAGTCCTTC TCATTGGTCA AGGCACTTTA CCATATTTCT TTGGCAATGT GGGTGATCTG 
GTGGTGAGCC CCCTGCTGGT GAGCTGCTAC AAGGGCAGAG AGCTCAACGA GAAGACCCTG 
GCCAGCCTGG GCATGTCAGC AAACCAGTTA CTGACCACAG AGACCATGAT ACTGCTCACA 
CTGCAGTACC TCGCTCGACT GGGTACAGAG CAGATACCGT TACGGGAAGA GTTTGAGCAA 
ATCATGTTGA AGGCTATGCT GTGTGGTCCG ACTGGCCCTC CAGTGTCGCC TGCGCAGCTG 
CCCTGGTTAG CCCGAATGGA GGCCAGTGTG TCAGGGGGCA GTGTTCAGGT GCTGGTCACC 
CACGGCTCTC TTGGTGAGGG TATCTCTGAG TCTCTGCGCT CACTGAGTGA AACATCACCA 
CAGCAGCAGC AGTGTCTCCC CAACTATGTG CTCATTATCT GCACCTCCAA AAGTGGAGCC 
AATGAGTTCT GTGTGCTCGT GTTGGGTAAG TATCAGTCTC GAGCCTTGGC AGAGAGCATG 
CTGTCTACAA ACGAGTTTCT GAAGGAGATC AGTTATGAGC TCATCACAGG GAAAGTTAGT 
GTACTAGCCT CACACTTCCA GAGCACCTCT TTAGGTGACA ATATGGACAA ACAGCTGGTT 
CGTTATCAAC GTAAACGCAA AGACAGGGTG GTTCAACCTT TTCAGGGGCA TCTCACCGAG 
TACATCCACT CCCAGGAAGC TGCGACCATG ATACCAGAAT CAGGGCCAGA TCTGCTGAGC 
GATGACTTTC AGATACACCC TCCTCAGTTG AGCGTTGCAC GAAGCCTTTT GTCACAGGTT 
TGTGCTATTG CCGACTCAGG GAGCCAGAGC TTAGATCTGG GCCGATTCTG CAAAGTAGAC 
TTTCTGATTC TGGTGCCGCC CTCCCATGTT CTGGTTCACC AAACAGTCCA GCGAATACGA 
CAATCAGGCG TCCTAATAGA TCTCGGTATA GAGGACGTGT CATTAGCCAT GCAGAAGTCT 
GACAAGTATG TGGTGCGTCT GGACACAGAG GTCCACACAA AAATGGAGGC CTTTATGAGG 
AAGGTCAAGC AGAACCCTTA TACACTCTTC GTCCTCATTC ATGACAACTC GCACGTGGAC 
TTGACAAGTG CTCTTTCAGG GTCGGTTTGT CATGGGGAGC TGCAGGGATT GGCTGATCGC 
GTGGTGAACT GTCCAGAGGT TCTGGAAGCA ATAAACTTAC TGGTGCTGCA GGTCAGCTGC 
TTCCCGTTCA CCCTGCAGAG CCGGCAGTCA CGAATCAGTA CCCAGAATGA AGTGCACTGG 
CCCGACACTG AGAACCAGCA GGGTGAAGCT TCTCCTAAAG ACTTGATCTA CTTTGGGCTG 
AAGGACTACA GCAAGTCTCT GCAGTGGGGA GTGGCGAGTC CAATTCTGCG ATGTGATGAC 
GCCTTTGAGC GGATGGTTAA AACCCTGTTG GAGAGGCACC CACATTTGCA CAGTATGGTG 
ATCCGCAGCT ACCTGTTGAT CCAGCAGTAC ACAGAGGCTC TGATGGCCCT CACAGCGGCC 
CCTTCTTTGC GGGATCACGT GACCCCTCAG ACACTTGCCA TGGTGGAGGA TCTGCTGAGT 
GTGCCAGGCC GCAGCAAGCA TGGGTGCGGA CACATGCTGC TGGTGCGTGT GCCTTCTCTG 
CAGCTGGCAC GACTGGCACA GGAGCGGCTG GAGGAGGCTC GGGACAAGCT GGGGCTGCAG 
TACCGCTTTG CCGTGCTGCT AGGAAGCCCT GCTGCGGAGA TCAGCCTGCC GGTGCACTTC 
TGTGCCCGTC TGCGGGCTTG GAGGGGCTGT AAAAATGAGG AGTGGGTCCC ACATACATAT 
GAAGATCTGG AAGGACTTCC ATGCATTGTC ATCCTCACTG GAAAAGATCC TCTTGGAGAA 
ACCTTCCCGA GGTCTCTAAA GTACTGTGAC TTGCGGCTGA TTGACTCCAG CTACCTGACA 
CGCACAGCGC TGGAACAGGA AGTGGGTTTG GCCTGTTCAT ATGTGACACG TAGAGTAATC 
CCTAAGACCA AAACTGCCAC AAGCAGAGAA GAGAGACCGA GAGAAGGTGA AAGGAGCTCT 
GGAGAAACAG CAGAACACGA TGATCTCCCT ATGGAGCTGG AAAGACCACC CAGCAATGCC 
AGCGCTGCTA CTAGGACCTC CGGCTCCACC ACAGAAAATG GTGTCAGTTC CTCAAGTATA 
CTTGACAAGC CATCCTCGCA GAGTGACCCT TGTGGAAGCA GGACTATGAT GGACTCTTGC 
TCCTCTCCTG TTCGCTTCAA GCAGGAGTGC GACTCTCAGG CTCCCTCATC ATCCAGCACG 
TCCTCATTCT CCTCTGCTTC CTCCTCCTCT TCTTCTTCCT CGTCTCCGGC AGCTCAGAGA 
CCGAGTCAGT CTACACAGGC TCCACGTGAA TGTAACAGGA CACAGGTCTT TCCCCGGACG 
GCGGTGCTGT CTCGGGCTGC GTATACCCTG CTGGCTCCTG AAACTCTGGG TCATCCCAGT 
TCTGCCTCTC TGCTGCCACA TGCGGATGTG TCGTGGAGCA GTCCGCTCAG ACCACCGGTT 
CCTCATGGCC TTGGTGGAGC AGAGCAGTCC CTCTACTATC GTCAGTGGAC CACAGCCAGG 
CAACACCACG CTGATTATGA AGGCCCAGTA CCCCATCCCC ACCCAAGAAG ACTGCTTCTG 
AGCGGCCCAC CACAGGTTGG AAAAACAGGT GCATATCTGC AGTTCCTACG AATTCTGTTC 
CGTATGCTGA TTCGCCTTCT GGAGGTAGAT GTTTATGATG AAGAAGAGCT GGAGGAAGAT 
GTGCAAGACA AATCAAAGGT TCCCCCTTCT AGTGGGCCAC AGTGGCCTGA TGTGGAGGAC 
GTCCGCAAAC TAAGATTTGA CCTCTGCCCT CATGACTGCA AGTTCAAATA TTCAAGTCCA 
GTGTATGCCA ACAGGATGCC AAAAACACAG AGTGGGGTTA AAACCGAAAG ACTGGATACA 
GAAGCGGACC CACCAAAGAG AAACACAGTA TCTGTTAGAC TCAGCCTTTT TGCAGCCCAC 
AATGCATTTC ACCACTGTGA GCAGTGCCAC CATTACAGTG AACCTATTCC TGCTGCTCAG 
CTCTCTGACT GCACCTTCCA CGCTTTCACC TTTTGCTCAT CTATGCTGGG AGAGGAAGTC 
CAGCTGCACT TTATCATCCC TAAATCCAAA GAGTCTCATT TTGTGTTCAG CCAACAGGGC 
AGTCACCTAG AGAGCATGAG ACTGCCTCTG CTTTCCGACA AGGAGTCGGG CATGATGAAA 
AGCCCAATAT TCACCCCAAC CACGGGCCGC CAGGAGCACG GCCTTCTCAA CATCTACCAT 
GCCATGGAGG GAGCCGAGCA CCTGCACATC CTGGTGGTCA AACAGTATGA AATGCCACTC 
TATAGGAAAT ACTGGCCCAA CCACATCCTG CTGGTGCTGC CCGCCATGTT CAACAACTCA 
GGAGTCGGTG CGGCTCGATT CATGATCAAA GAACTGTCCT ATCACAACCT GGAGCTTGAG 
AGGAACCGAC AAGAGGAGCA AGGCGTCAAA CGGCAGGACG TGTGGCCCTT TATCGTAATG 
ATGGATGACT CCTGCGTCCT GTGGAACGCC CAGCAGCCTG GACCAGACGG CAAAACCGAG 
GTGATGAACG TGTCTCTGAA GAGCGTCTTG CAGCACATGG AAGCCACTCC AAAGATCTCC 
CAGTATGCAG TTTGTGGCCT GCGCAAATGG AGCAGTAGTT TGTCTTCTCA AGCTCCCACA 
AGCCCTTTCT CTCGCTGTCA TCTCCACGAC CTCATCTTGC TGAATGTTGA CCTCACACAG 
AACGTCCAGT ATGACCTCAA TCGGTTCACA TGTGAAGAGG TGGACTTCAA CCTGAGGGCA 
AACAGCAGTG GTCTTCTGCT CTGTCGCTTC AATCAATTCA GCATCATGAA GAAACACATT 
CCCATCGGAG GACATAAAGA CTTCCTCATC AAACCCAAGC TCATGAGAAT AGAGACGCCG 
GTGCGTGTGT GTGCGTCTCA ATATGTGTGC GCTCCGGACA GTGAACAAAC GTTGCTGGCT 
GCGCCAGCTC AGTTCCTGCT GGAGAAGTTT CTGCAGTCCT GTAGCCATCG GCTCTTTCCT 
CTGGCTCTCA GTAACAGTGC TAACCCTGTG TTGTCCATAG ACAGCTACCT CAACCTTGGA 
CCAGAGGTTC AGGTGTGTTA TGTGAGCTCG CGACCTCATT CGGTGAATGT AGATCATCAA 
GGTGTCATTT TCAGTGGCCT GCTGCTCTAC TTGTGTGACT CCTTTGTCGT TTCAAGCCTT 
CTAAAGAAAT TCAACTTCCT CAAAGGCGCC ACGCTGTGTG TGATCTGTCA GGACCGGAGC 
TCTCTGCGTC AGACTATTGT ACGTCTGGAG CTGGAGGATG AGTGGCAATT TCGCCTGCGA 
GATGAGTTTC AAACGGCCAA CTGCAGCGAG GACCGTCCGC TCTACTTCCT CACAGGACGA 
CATATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296315.1
CDS	216..6044
Translation	MGNSYAGQLKSARFEEALHNSIEASLRSSGGDPQPVFTQLYLEPDQYSGHVEDIKPKMDLSLRSDPSTHVLVKCHSSNSVEDMDDEDDSDTSSPPLPYLQGPPPDGCCTVDGFCQAGKDLRLVSMATESIEVPAGFELVGAKSPSIPEHILVCAVDKRFLPDENGKNALLGFSGNCVGCGEKGFRYFTEFSNHINLKLSTQPKKQKHLKYYLVKNSQGALCKGALICWKDCKTRPFSNSASSSKPSSSSSLSSKENGDTNGHSPSPFPLSDSPPARMQSGSSSGIFGPQELGFLKPLNTPTHGTKTLPIVPTALRVNGLTNGLSMDGRSTLLSPPRTNPLSTPSHGYRTTETGDSPASTAMSTGPPKKRHRSWHPTTLVPIPATAVPVPAIRPLTCSSGPLLSLSNQQPASVSGVIQPQPITAGETVIIPDNLLNSYGVRPVLLIGQGTLPYFFGNVGDLVVSPLLVSCYKGRELNEKTLASLGMSANQLLTTETMILLTLQYLARLGTEQIPLREEFEQIMLKAMLCGPTGPPVSPAQLPWLARMEASVSGGSVQVLVTHGSLGEGISESLRSLSETSPQQQQCLPNYVLIICTSKSGANEFCVLVLGKYQSRALAESMLSTNEFLKEISYELITGKVSVLASHFQSTSLGDNMDKQLVRYQRKRKDRVVQPFQGHLTEYIHSQEAATMIPESGPDLLSDDFQIHPPQLSVARSLLSQVCAIADSGSQSLDLGRFCKVDFLILVPPSHVLVHQTVQRIRQSGVLIDLGIEDVSLAMQKSDKYVVRLDTEVHTKMEAFMRKVKQNPYTLFVLIHDNSHVDLTSALSGSVCHGELQGLADRVVNCPEVLEAINLLVLQVSCFPFTLQSRQSRISTQNEVHWPDTENQQGEASPKDLIYFGLKDYSKSLQWGVASPILRCDDAFERMVKTLLERHPHLHSMVIRSYLLIQQYTEALMALTAAPSLRDHVTPQTLAMVEDLLSVPGRSKHGCGHMLLVRVPSLQLARLAQERLEEARDKLGLQYRFAVLLGSPAAEISLPVHFCARLRAWRGCKNEEWVPHTYEDLEGLPCIVILTGKDPLGETFPRSLKYCDLRLIDSSYLTRTALEQEVGLACSYVTRRVIPKTKTATSREERPREGERSSGETAEHDDLPMELERPPSNASAATRTSGSTTENGVSSSSILDKPSSQSDPCGSRTMMDSCSSPVRFKQECDSQAPSSSSTSSFSSASSSSSSSSSPAAQRPSQSTQAPRECNRTQVFPRTAVLSRAAYTLLAPETLGHPSSASLLPHADVSWSSPLRPPVPHGLGGAEQSLYYRQWTTARQHHADYEGPVPHPHPRRLLLSGPPQVGKTGAYLQFLRILFRMLIRLLEVDVYDEEELEEDVQDKSKVPPSSGPQWPDVEDVRKLRFDLCPHDCKFKYSSPVYANRMPKTQSGVKTERLDTEADPPKRNTVSVRLSLFAAHNAFHHCEQCHHYSEPIPAAQLSDCTFHAFTFCSSMLGEEVQLHFIIPKSKESHFVFSQQGSHLESMRLPLLSDKESGMMKSPIFTPTTGRQEHGLLNIYHAMEGAEHLHILVVKQYEMPLYRKYWPNHILLVLPAMFNNSGVGAARFMIKELSYHNLELERNRQEEQGVKRQDVWPFIVMMDDSCVLWNAQQPGPDGKTEVMNVSLKSVLQHMEATPKISQYAVCGLRKWSSSLSSQAPTSPFSRCHLHDLILLNVDLTQNVQYDLNRFTCEEVDFNLRANSSGLLLCRFNQFSIMKKHIPIGGHKDFLIKPKLMRIETPVRVCASQYVCAPDSEQTLLAAPAQFLLEKFLQSCSHRLFPLALSNSANPVLSIDSYLNLGPEVQVCYVSSRPHSVNVDHQGVIFSGLLLYLCDSFVVSSLLKKFNFLKGATLCVICQDRSSLRQTIVRLELEDEWQFRLRDEFQTANCSEDRPLYFLTGRHI

Target ORF information:

RefSeq Version	XM_009298040.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio growth regulation by estrogen in breast cancer-like (greb1l), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298040.1

ORF Insert Sequence:

ATGGGAAATT CATACGCGGG GCAGCTGAAA TCTGCTCGCT TTGAGGAAGC TCTCCATAAC 
TCAATAGAGG CTTCATTAAG GTCAAGCGGT GGTGACCCAC AGCCGGTTTT CACACAGCTA 
TATCTCGAGC CTGATCAGTA TTCTGGCCAT GTAGAAGATA TTAAGCCCAA AATGGACCTA 
AGCCTGAGAT CCGACCCATC CACCCATGTG CTTGTGAAAT GCCACTCTTC AAATTCTGTA 
GAGGATATGG ATGACGAGGA CGATTCAGAC ACCAGCAGCC CACCATTGCC TTACCTGCAG 
GGACCCCCTC CTGATGGCTG CTGCACTGTT GATGGTTTCT GTCAAGCAGG AAAAGATCTG 
CGCCTGGTTT CCATGGCAAC AGAGTCTATC GAGGTTCCTG CAGGGTTTGA GCTGGTCGGT 
GCCAAATCGC CCAGCATCCC AGAGCACATC CTCGTCTGTG CAGTGGACAA GCGCTTCCTG 
CCAGATGAGA ATGGGAAAAA TGCACTTTTA GGGTTTTCAG GGAACTGCGT GGGTTGTGGT 
GAGAAAGGGT TCCGTTACTT CACCGAGTTC TCTAACCACA TTAACCTGAA GTTATCCACT 
CAGCCGAAGA AGCAGAAGCA CTTAAAGTAC TACCTGGTGA AGAACTCTCA GGGTGCTCTG 
TGCAAAGGAG CCCTCATTTG CTGGAAAGAT TGTAAGACAC GACCATTCTC AAACAGTGCT 
TCTTCATCCA AGCCTAGTTC CTCATCCTCA CTAAGCAGCA AAGAAAATGG AGACACAAAT 
GGCCACAGTC CATCTCCCTT CCCCCTCTCA GACTCGCCTC CTGCCCGCAT GCAATCTGGC 
TCATCCTCAG GGATTTTTGG ACCCCAGGAG CTGGGCTTCC TTAAACCATT GAACACACCG 
ACCCACGGGA CCAAGACATT ACCGATCGTT CCCACTGCCC TCCGAGTGAA TGGTCTAACT 
AATGGTCTCA GTATGGATGG ACGCTCCACT CTACTGAGCC CACCTCGTAC AAACCCACTC 
TCCACCCCTA GTCATGGCTA TAGAACCACA GAAACAGGAG ACAGTCCAGC ATCCACTGCT 
ATGTCGACAG GTCCACCAAA AAAGCGGCAT CGTAGTTGGC ATCCCACCAC TTTAGTGCCC 
ATCCCAGCCA CAGCTGTTCC TGTACCAGCC ATCCGACCGC TGACCTGCAG CTCTGGTCCT 
TTATTGTCAC TGTCCAATCA ACAGCCTGCA TCGGTGTCAG GGGTCATACA ACCACAGCCA 
ATCACAGCTG GAGAAACGGT CATCATTCCT GATAACCTTC TGAACTCTTA CGGGGTCCGG 
CCAGTCCTTC TCATTGGTCA AGGCACTTTA CCATATTTCT TTGGCAATGT GGGTGATCTG 
GTGGTGAGCC CCCTGCTGGT GAGCTGCTAC AAGGGCAGAG AGCTCAACGA GAAGACCCTG 
GCCAGCCTGG GCATGTCAGC AAACCAGTTA CTGACCACAG AGACCATGAT ACTGCTCACA 
CTGCAGTACC TCGCTCGACT GGGTACAGAG CAGATACCGT TACGGGAAGA GTTTGAGCAA 
ATCATGTTGA AGGCTATGCT GTGTGGTCCG ACTGGCCCTC CAGTGTCGCC TGCGCAGCTG 
CCCTGGTTAG CCCGAATGGA GGCCAGTGTG TCAGGGGGCA GTGTTCAGGT GCTGGTCACC 
CACGGCTCTC TTGGTGAGGG TATCTCTGAG TCTCTGCGCT CACTGAGTGA AACATCACCA 
CAGCAGCAGC AGTGTCTCCC CAACTATGTG CTCATTATCT GCACCTCCAA AAGTGGAGCC 
AATGAGTTCT GTGTGCTCGT GTTGGGTAAG TATCAGTCTC GAGCCTTGGC AGAGAGCATG 
CTGTCTACAA ACGAGTTTCT GAAGGAGATC AGTTATGAGC TCATCACAGG GAAAGTTAGT 
GTACTAGCCT CACACTTCCA GAGCACCTCT TTAGGTGACA ATATGGACAA ACAGCTGGTT 
CGTTATCAAC GTAAACGCAA AGACAGGGTG GTTCAACCTT TTCAGGGGCA TCTCACCGAG 
TACATCCACT CCCAGGAAGC TGCGACCATG ATACCAGAAT CAGGGCCAGA TCTGCTGAGC 
GATGACTTTC AGATACACCC TCCTCAGTTG AGCGTTGCAC GAAGCCTTTT GTCACAGGTT 
TGTGCTATTG CCGACTCAGG GAGCCAGAGC TTAGATCTGG GCCGATTCTG CAAAGTAGAC 
TTTCTGATTC TGGTGCCGCC CTCCCATGTT CTGGTTCACC AAACAGTCCA GCGAATACGA 
CAATCAGGCG TCCTAATAGA TCTCGGTATA GAGGACGTGT CATTAGCCAT GCAGAAGTCT 
GACAAGTATG TGGTGCGTCT GGACACAGAG GTCCACACAA AAATGGAGGC CTTTATGAGG 
AAGGTCAAGC AGAACCCTTA TACACTCTTC GTCCTCATTC ATGACAACTC GCACGTGGAC 
TTGACAAGTG CTCTTTCAGG GTCGGTTTGT CATGGGGAGC TGCAGGGATT GGCTGATCGC 
GTGGTGAACT GTCCAGAGGT TCTGGAAGCA ATAAACTTAC TGGTGCTGCA GGTCAGCTGC 
TTCCCGTTCA CCCTGCAGAG CCGGCAGTCA CGAATCAGTA CCCAGAATGA AGTGCACTGG 
CCCGACACTG AGAACCAGCA GGGTGAAGCT TCTCCTAAAG ACTTGATCTA CTTTGGGCTG 
AAGGACTACA GCAAGTCTCT GCAGTGGGGA GTGGCGAGTC CAATTCTGCG ATGTGATGAC 
GCCTTTGAGC GGATGGTTAA AACCCTGTTG GAGAGGCACC CACATTTGCA CAGTATGGTG 
ATCCGCAGCT ACCTGTTGAT CCAGCAGTAC ACAGAGGCTC TGATGGCCCT CACAGCGGCC 
CCTTCTTTGC GGGATCACGT GACCCCTCAG ACACTTGCCA TGGTGGAGGA TCTGCTGAGT 
GTGCCAGGCC GCAGCAAGCA TGGGTGCGGA CACATGCTGC TGGTGCGTGT GCCTTCTCTG 
CAGCTGGCAC GACTGGCACA GGAGCGGCTG GAGGAGGCTC GGGACAAGCT GGGGCTGCAG 
TACCGCTTTG CCGTGCTGCT AGGAAGCCCT GCTGCGGAGA TCAGCCTGCC GGTGCACTTC 
TGTGCCCGTC TGCGGGCTTG GAGGGGCTGT AAAAATGAGG AGTGGGTCCC ACATACATAT 
GAAGATCTGG AAGGACTTCC ATGCATTGTC ATCCTCACTG GAAAAGATCC TCTTGGAGAA 
ACCTTCCCGA GGTCTCTAAA GTACTGTGAC TTGCGGCTGA TTGACTCCAG CTACCTGACA 
CGCACAGCGC TGGAACAGGA AGTGGGTTTG GCCTGTTCAT ATGTGACACG TAGAGTAATC 
CCTAAGACCA AAACTGCCAC AAGCAGAGAA GAGAGACCGA GAGAAGGTGA AAGGAGCTCT 
GGAGAAACAG CAGAACACGA TGATCTCCCT ATGGAGCTGG AAAGACCACC CAGCAATGCC 
AGCGCTGCTA CTAGGACCTC CGGCTCCACC ACAGAAAATG GTGTCAGTTC CTCAAGTATA 
CTTGACAAGC CATCCTCGCA GAGTGACCCT TGTGGAAGCA GGACTATGAT GGACTCTTGC 
TCCTCTCCTG TTCGCTTCAA GCAGGAGTGC GACTCTCAGG CTCCCTCATC ATCCAGCACG 
TCCTCATTCT CCTCTGCTTC CTCCTCCTCT TCTTCTTCCT CGTCTCCGGC AGCTCAGAGA 
CCGAGTCAGT CTACACAGGC TCCACGTGAA TGTAACAGGA CACAGGTCTT TCCCCGGACG 
GCGGTGCTGT CTCGGGCTGC GTATACCCTG CTGGCTCCTG AAACTCTGGG TCATCCCAGT 
TCTGCCTCTC TGCTGCCACA TGCGGATGTG TCGTGGAGCA GTCCGCTCAG ACCACCGGTT 
CCTCATGGCC TTGGTGGAGC AGAGCAGTCC CTCTACTATC GTCAGTGGAC CACAGCCAGG 
CAACACCACG CTGATTATGA AGGCCCAGTA CCCCATCCCC ACCCAAGAAG ACTGCTTCTG 
AGCGGCCCAC CACAGGTTGG AAAAACAGGT GCATATCTGC AGTTCCTACG AATTCTGTTC 
CGTATGCTGA TTCGCCTTCT GGAGGTAGAT GTTTATGATG AAGAAGAGCT GGAGGAAGAT 
GTGCAAGACA AATCAAAGGT TCCCCCTTCT AGTGGGCCAC AGTGGCCTGA TGTGGAGGAC 
GTCCGCAAAC TAAGATTTGA CCTCTGCCCT CATGACTGCA AGTTCAAATA TTCAAGTCCA 
GTGTATGCCA ACAGGATGCC AAAAACACAG AGTGGGGTTA AAACCGAAAG ACTGGATACA 
GAAGCGGACC CACCAAAGAG AAACACAGTA TCTGTTAGAC TCAGCCTTTT TGCAGCCCAC 
AATGCATTTC ACCACTGTGA GCAGTGCCAC CATTACAGTG AACCTATTCC TGCTGCTCAG 
CTCTCTGACT GCACCTTCCA CGCTTTCACC TTTTGCTCAT CTATGCTGGG AGAGGAAGTC 
CAGCTGCACT TTATCATCCC TAAATCCAAA GAGTCTCATT TTGTGTTCAG CCAACAGGGC 
AGTCACCTAG AGAGCATGAG ACTGCCTCTG CTTTCCGACA AGGAGTCGGG CATGATGAAA 
AGCCCAATAT TCACCCCAAC CACGGGCCGC CAGGAGCACG GCCTTCTCAA CATCTACCAT 
GCCATGGAGG GAGCCGAGCA CCTGCACATC CTGGTGGTCA AACAGTATGA AATGCCACTC 
TATAGGAAAT ACTGGCCCAA CCACATCCTG CTGGTGCTGC CCGCCATGTT CAACAACTCA 
GGAGTCGGTG CGGCTCGATT CATGATCAAA GAACTGTCCT ATCACAACCT GGAGCTTGAG 
AGGAACCGAC AAGAGGAGCA AGGCGTCAAA CGGCAGGACG TGTGGCCCTT TATCGTAATG 
ATGGATGACT CCTGCGTCCT GTGGAACGCC CAGCAGCCTG GACCAGACGG CAAAACCGAG 
GTGATGAACG TGTCTCTGAA GAGCGTCTTG CAGCACATGG AAGCCACTCC AAAGATCTCC 
CAGTATGCAG TTTGTGGCCT GCGCAAATGG AGCAGTAGTT TGTCTTCTCA AGCTCCCACA 
AGCCCTTTCT CTCGCTGTCA TCTCCACGAC CTCATCTTGC TGAATGTTGA CCTCACACAG 
AACGTCCAGT ATGACCTCAA TCGGTTCACA TGTGAAGAGG TGGACTTCAA CCTGAGGGCA 
AACAGCAGTG GTCTTCTGCT CTGTCGCTTC AATCAATTCA GCATCATGAA GAAACACATT 
CCCATCGGAG GACATAAAGA CTTCCTCATC AAACCCAAGC TCATGAGAAT AGAGACGCCG 
GTGCGTGTGT GTGCGTCTCA ATATGTGTGC GCTCCGGACA GTGAACAAAC GTTGCTGGCT 
GCGCCAGCTC AGTTCCTGCT GGAGAAGTTT CTGCAGTCCT GTAGCCATCG GCTCTTTCCT 
CTGGCTCTCA GTAACAGTGC TAACCCTGTG TTGTCCATAG ACAGCTACCT CAACCTTGGA 
CCAGAGGTTC AGGTGTGTTA TGTGAGCTCG CGACCTCATT CGGTGAATGT AGATCATCAA 
GGTGTCATTT TCAGTGGCCT GCTGCTCTAC TTGTGTGACT CCTTTGTCGT TTCAAGCCTT 
CTAAAGAAAT TCAACTTCCT CAAAGGCGCC ACGCTGTGTG TGATCTGTCA GGACCGGAGC 
TCTCTGCGTC AGACTATTGT ACGTCTGGAG CTGGAGGATG AGTGGCAATT TCGCCTGCGA 
GATGAGTTTC AAACGGCCAA CTGCAGCGAG GACCGTCCGC TCTACTTCCT CACAGGACGA 
CATATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GREB1L ( XM_009298040.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009296315.1 Danio rerio growth regulation by estrogen in breast cancer-like (greb1l), transcript variant X2, mRNA.

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CloneID	ODa06787
Clone ID Related Accession (Same CDS sequence)	XM_001344416.8 , XM_009298040.1 , XM_009298040.2 , XM_021466912.1 , NM_001362307.1 , XM_001344416.7
Accession Version	XM_009298040.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	GREB1-like protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298040.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAATT CATACGCGGG GCAGCTGAAA TCTGCTCGCT TTGAGGAAGC TCTCCATAAC 
TCAATAGAGG CTTCATTAAG GTCAAGCGGT GGTGACCCAC AGCCGGTTTT CACACAGCTA 
TATCTCGAGC CTGATCAGTA TTCTGGCCAT GTAGAAGATA TTAAGCCCAA AATGGACCTA 
AGCCTGAGAT CCGACCCATC CACCCATGTG CTTGTGAAAT GCCACTCTTC AAATTCTGTA 
GAGGATATGG ATGACGAGGA CGATTCAGAC ACCAGCAGCC CACCATTGCC TTACCTGCAG 
GGACCCCCTC CTGATGGCTG CTGCACTGTT GATGGTTTCT GTCAAGCAGG AAAAGATCTG 
CGCCTGGTTT CCATGGCAAC AGAGTCTATC GAGGTTCCTG CAGGGTTTGA GCTGGTCGGT 
GCCAAATCGC CCAGCATCCC AGAGCACATC CTCGTCTGTG CAGTGGACAA GCGCTTCCTG 
CCAGATGAGA ATGGGAAAAA TGCACTTTTA GGGTTTTCAG GGAACTGCGT GGGTTGTGGT 
GAGAAAGGGT TCCGTTACTT CACCGAGTTC TCTAACCACA TTAACCTGAA GTTATCCACT 
CAGCCGAAGA AGCAGAAGCA CTTAAAGTAC TACCTGGTGA AGAACTCTCA GGGTGCTCTG 
TGCAAAGGAG CCCTCATTTG CTGGAAAGAT TGTAAGACAC GACCATTCTC AAACAGTGCT 
TCTTCATCCA AGCCTAGTTC CTCATCCTCA CTAAGCAGCA AAGAAAATGG AGACACAAAT 
GGCCACAGTC CATCTCCCTT CCCCCTCTCA GACTCGCCTC CTGCCCGCAT GCAATCTGGC 
TCATCCTCAG GGATTTTTGG ACCCCAGGAG CTGGGCTTCC TTAAACCATT GAACACACCG 
ACCCACGGGA CCAAGACATT ACCGATCGTT CCCACTGCCC TCCGAGTGAA TGGTCTAACT 
AATGGTCTCA GTATGGATGG ACGCTCCACT CTACTGAGCC CACCTCGTAC AAACCCACTC 
TCCACCCCTA GTCATGGCTA TAGAACCACA GAAACAGGAG ACAGTCCAGC ATCCACTGCT 
ATGTCGACAG GTCCACCAAA AAAGCGGCAT CGTAGTTGGC ATCCCACCAC TTTAGTGCCC 
ATCCCAGCCA CAGCTGTTCC TGTACCAGCC ATCCGACCGC TGACCTGCAG CTCTGGTCCT 
TTATTGTCAC TGTCCAATCA ACAGCCTGCA TCGGTGTCAG GGGTCATACA ACCACAGCCA 
ATCACAGCTG GAGAAACGGT CATCATTCCT GATAACCTTC TGAACTCTTA CGGGGTCCGG 
CCAGTCCTTC TCATTGGTCA AGGCACTTTA CCATATTTCT TTGGCAATGT GGGTGATCTG 
GTGGTGAGCC CCCTGCTGGT GAGCTGCTAC AAGGGCAGAG AGCTCAACGA GAAGACCCTG 
GCCAGCCTGG GCATGTCAGC AAACCAGTTA CTGACCACAG AGACCATGAT ACTGCTCACA 
CTGCAGTACC TCGCTCGACT GGGTACAGAG CAGATACCGT TACGGGAAGA GTTTGAGCAA 
ATCATGTTGA AGGCTATGCT GTGTGGTCCG ACTGGCCCTC CAGTGTCGCC TGCGCAGCTG 
CCCTGGTTAG CCCGAATGGA GGCCAGTGTG TCAGGGGGCA GTGTTCAGGT GCTGGTCACC 
CACGGCTCTC TTGGTGAGGG TATCTCTGAG TCTCTGCGCT CACTGAGTGA AACATCACCA 
CAGCAGCAGC AGTGTCTCCC CAACTATGTG CTCATTATCT GCACCTCCAA AAGTGGAGCC 
AATGAGTTCT GTGTGCTCGT GTTGGGTAAG TATCAGTCTC GAGCCTTGGC AGAGAGCATG 
CTGTCTACAA ACGAGTTTCT GAAGGAGATC AGTTATGAGC TCATCACAGG GAAAGTTAGT 
GTACTAGCCT CACACTTCCA GAGCACCTCT TTAGGTGACA ATATGGACAA ACAGCTGGTT 
CGTTATCAAC GTAAACGCAA AGACAGGGTG GTTCAACCTT TTCAGGGGCA TCTCACCGAG 
TACATCCACT CCCAGGAAGC TGCGACCATG ATACCAGAAT CAGGGCCAGA TCTGCTGAGC 
GATGACTTTC AGATACACCC TCCTCAGTTG AGCGTTGCAC GAAGCCTTTT GTCACAGGTT 
TGTGCTATTG CCGACTCAGG GAGCCAGAGC TTAGATCTGG GCCGATTCTG CAAAGTAGAC 
TTTCTGATTC TGGTGCCGCC CTCCCATGTT CTGGTTCACC AAACAGTCCA GCGAATACGA 
CAATCAGGCG TCCTAATAGA TCTCGGTATA GAGGACGTGT CATTAGCCAT GCAGAAGTCT 
GACAAGTATG TGGTGCGTCT GGACACAGAG GTCCACACAA AAATGGAGGC CTTTATGAGG 
AAGGTCAAGC AGAACCCTTA TACACTCTTC GTCCTCATTC ATGACAACTC GCACGTGGAC 
TTGACAAGTG CTCTTTCAGG GTCGGTTTGT CATGGGGAGC TGCAGGGATT GGCTGATCGC 
GTGGTGAACT GTCCAGAGGT TCTGGAAGCA ATAAACTTAC TGGTGCTGCA GGTCAGCTGC 
TTCCCGTTCA CCCTGCAGAG CCGGCAGTCA CGAATCAGTA CCCAGAATGA AGTGCACTGG 
CCCGACACTG AGAACCAGCA GGGTGAAGCT TCTCCTAAAG ACTTGATCTA CTTTGGGCTG 
AAGGACTACA GCAAGTCTCT GCAGTGGGGA GTGGCGAGTC CAATTCTGCG ATGTGATGAC 
GCCTTTGAGC GGATGGTTAA AACCCTGTTG GAGAGGCACC CACATTTGCA CAGTATGGTG 
ATCCGCAGCT ACCTGTTGAT CCAGCAGTAC ACAGAGGCTC TGATGGCCCT CACAGCGGCC 
CCTTCTTTGC GGGATCACGT GACCCCTCAG ACACTTGCCA TGGTGGAGGA TCTGCTGAGT 
GTGCCAGGCC GCAGCAAGCA TGGGTGCGGA CACATGCTGC TGGTGCGTGT GCCTTCTCTG 
CAGCTGGCAC GACTGGCACA GGAGCGGCTG GAGGAGGCTC GGGACAAGCT GGGGCTGCAG 
TACCGCTTTG CCGTGCTGCT AGGAAGCCCT GCTGCGGAGA TCAGCCTGCC GGTGCACTTC 
TGTGCCCGTC TGCGGGCTTG GAGGGGCTGT AAAAATGAGG AGTGGGTCCC ACATACATAT 
GAAGATCTGG AAGGACTTCC ATGCATTGTC ATCCTCACTG GAAAAGATCC TCTTGGAGAA 
ACCTTCCCGA GGTCTCTAAA GTACTGTGAC TTGCGGCTGA TTGACTCCAG CTACCTGACA 
CGCACAGCGC TGGAACAGGA AGTGGGTTTG GCCTGTTCAT ATGTGACACG TAGAGTAATC 
CCTAAGACCA AAACTGCCAC AAGCAGAGAA GAGAGACCGA GAGAAGGTGA AAGGAGCTCT 
GGAGAAACAG CAGAACACGA TGATCTCCCT ATGGAGCTGG AAAGACCACC CAGCAATGCC 
AGCGCTGCTA CTAGGACCTC CGGCTCCACC ACAGAAAATG GTGTCAGTTC CTCAAGTATA 
CTTGACAAGC CATCCTCGCA GAGTGACCCT TGTGGAAGCA GGACTATGAT GGACTCTTGC 
TCCTCTCCTG TTCGCTTCAA GCAGGAGTGC GACTCTCAGG CTCCCTCATC ATCCAGCACG 
TCCTCATTCT CCTCTGCTTC CTCCTCCTCT TCTTCTTCCT CGTCTCCGGC AGCTCAGAGA 
CCGAGTCAGT CTACACAGGC TCCACGTGAA TGTAACAGGA CACAGGTCTT TCCCCGGACG 
GCGGTGCTGT CTCGGGCTGC GTATACCCTG CTGGCTCCTG AAACTCTGGG TCATCCCAGT 
TCTGCCTCTC TGCTGCCACA TGCGGATGTG TCGTGGAGCA GTCCGCTCAG ACCACCGGTT 
CCTCATGGCC TTGGTGGAGC AGAGCAGTCC CTCTACTATC GTCAGTGGAC CACAGCCAGG 
CAACACCACG CTGATTATGA AGGCCCAGTA CCCCATCCCC ACCCAAGAAG ACTGCTTCTG 
AGCGGCCCAC CACAGGTTGG AAAAACAGGT GCATATCTGC AGTTCCTACG AATTCTGTTC 
CGTATGCTGA TTCGCCTTCT GGAGGTAGAT GTTTATGATG AAGAAGAGCT GGAGGAAGAT 
GTGCAAGACA AATCAAAGGT TCCCCCTTCT AGTGGGCCAC AGTGGCCTGA TGTGGAGGAC 
GTCCGCAAAC TAAGATTTGA CCTCTGCCCT CATGACTGCA AGTTCAAATA TTCAAGTCCA 
GTGTATGCCA ACAGGATGCC AAAAACACAG AGTGGGGTTA AAACCGAAAG ACTGGATACA 
GAAGCGGACC CACCAAAGAG AAACACAGTA TCTGTTAGAC TCAGCCTTTT TGCAGCCCAC 
AATGCATTTC ACCACTGTGA GCAGTGCCAC CATTACAGTG AACCTATTCC TGCTGCTCAG 
CTCTCTGACT GCACCTTCCA CGCTTTCACC TTTTGCTCAT CTATGCTGGG AGAGGAAGTC 
CAGCTGCACT TTATCATCCC TAAATCCAAA GAGTCTCATT TTGTGTTCAG CCAACAGGGC 
AGTCACCTAG AGAGCATGAG ACTGCCTCTG CTTTCCGACA AGGAGTCGGG CATGATGAAA 
AGCCCAATAT TCACCCCAAC CACGGGCCGC CAGGAGCACG GCCTTCTCAA CATCTACCAT 
GCCATGGAGG GAGCCGAGCA CCTGCACATC CTGGTGGTCA AACAGTATGA AATGCCACTC 
TATAGGAAAT ACTGGCCCAA CCACATCCTG CTGGTGCTGC CCGCCATGTT CAACAACTCA 
GGAGTCGGTG CGGCTCGATT CATGATCAAA GAACTGTCCT ATCACAACCT GGAGCTTGAG 
AGGAACCGAC AAGAGGAGCA AGGCGTCAAA CGGCAGGACG TGTGGCCCTT TATCGTAATG 
ATGGATGACT CCTGCGTCCT GTGGAACGCC CAGCAGCCTG GACCAGACGG CAAAACCGAG 
GTGATGAACG TGTCTCTGAA GAGCGTCTTG CAGCACATGG AAGCCACTCC AAAGATCTCC 
CAGTATGCAG TTTGTGGCCT GCGCAAATGG AGCAGTAGTT TGTCTTCTCA AGCTCCCACA 
AGCCCTTTCT CTCGCTGTCA TCTCCACGAC CTCATCTTGC TGAATGTTGA CCTCACACAG 
AACGTCCAGT ATGACCTCAA TCGGTTCACA TGTGAAGAGG TGGACTTCAA CCTGAGGGCA 
AACAGCAGTG GTCTTCTGCT CTGTCGCTTC AATCAATTCA GCATCATGAA GAAACACATT 
CCCATCGGAG GACATAAAGA CTTCCTCATC AAACCCAAGC TCATGAGAAT AGAGACGCCG 
GTGCGTGTGT GTGCGTCTCA ATATGTGTGC GCTCCGGACA GTGAACAAAC GTTGCTGGCT 
GCGCCAGCTC AGTTCCTGCT GGAGAAGTTT CTGCAGTCCT GTAGCCATCG GCTCTTTCCT 
CTGGCTCTCA GTAACAGTGC TAACCCTGTG TTGTCCATAG ACAGCTACCT CAACCTTGGA 
CCAGAGGTTC AGGTGTGTTA TGTGAGCTCG CGACCTCATT CGGTGAATGT AGATCATCAA 
GGTGTCATTT TCAGTGGCCT GCTGCTCTAC TTGTGTGACT CCTTTGTCGT TTCAAGCCTT 
CTAAAGAAAT TCAACTTCCT CAAAGGCGCC ACGCTGTGTG TGATCTGTCA GGACCGGAGC 
TCTCTGCGTC AGACTATTGT ACGTCTGGAG CTGGAGGATG AGTGGCAATT TCGCCTGCGA 
GATGAGTTTC AAACGGCCAA CTGCAGCGAG GACCGTCCGC TCTACTTCCT CACAGGACGA 
CATATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296315.1
CDS	227..6055
Translation	MGNSYAGQLKSARFEEALHNSIEASLRSSGGDPQPVFTQLYLEPDQYSGHVEDIKPKMDLSLRSDPSTHVLVKCHSSNSVEDMDDEDDSDTSSPPLPYLQGPPPDGCCTVDGFCQAGKDLRLVSMATESIEVPAGFELVGAKSPSIPEHILVCAVDKRFLPDENGKNALLGFSGNCVGCGEKGFRYFTEFSNHINLKLSTQPKKQKHLKYYLVKNSQGALCKGALICWKDCKTRPFSNSASSSKPSSSSSLSSKENGDTNGHSPSPFPLSDSPPARMQSGSSSGIFGPQELGFLKPLNTPTHGTKTLPIVPTALRVNGLTNGLSMDGRSTLLSPPRTNPLSTPSHGYRTTETGDSPASTAMSTGPPKKRHRSWHPTTLVPIPATAVPVPAIRPLTCSSGPLLSLSNQQPASVSGVIQPQPITAGETVIIPDNLLNSYGVRPVLLIGQGTLPYFFGNVGDLVVSPLLVSCYKGRELNEKTLASLGMSANQLLTTETMILLTLQYLARLGTEQIPLREEFEQIMLKAMLCGPTGPPVSPAQLPWLARMEASVSGGSVQVLVTHGSLGEGISESLRSLSETSPQQQQCLPNYVLIICTSKSGANEFCVLVLGKYQSRALAESMLSTNEFLKEISYELITGKVSVLASHFQSTSLGDNMDKQLVRYQRKRKDRVVQPFQGHLTEYIHSQEAATMIPESGPDLLSDDFQIHPPQLSVARSLLSQVCAIADSGSQSLDLGRFCKVDFLILVPPSHVLVHQTVQRIRQSGVLIDLGIEDVSLAMQKSDKYVVRLDTEVHTKMEAFMRKVKQNPYTLFVLIHDNSHVDLTSALSGSVCHGELQGLADRVVNCPEVLEAINLLVLQVSCFPFTLQSRQSRISTQNEVHWPDTENQQGEASPKDLIYFGLKDYSKSLQWGVASPILRCDDAFERMVKTLLERHPHLHSMVIRSYLLIQQYTEALMALTAAPSLRDHVTPQTLAMVEDLLSVPGRSKHGCGHMLLVRVPSLQLARLAQERLEEARDKLGLQYRFAVLLGSPAAEISLPVHFCARLRAWRGCKNEEWVPHTYEDLEGLPCIVILTGKDPLGETFPRSLKYCDLRLIDSSYLTRTALEQEVGLACSYVTRRVIPKTKTATSREERPREGERSSGETAEHDDLPMELERPPSNASAATRTSGSTTENGVSSSSILDKPSSQSDPCGSRTMMDSCSSPVRFKQECDSQAPSSSSTSSFSSASSSSSSSSSPAAQRPSQSTQAPRECNRTQVFPRTAVLSRAAYTLLAPETLGHPSSASLLPHADVSWSSPLRPPVPHGLGGAEQSLYYRQWTTARQHHADYEGPVPHPHPRRLLLSGPPQVGKTGAYLQFLRILFRMLIRLLEVDVYDEEELEEDVQDKSKVPPSSGPQWPDVEDVRKLRFDLCPHDCKFKYSSPVYANRMPKTQSGVKTERLDTEADPPKRNTVSVRLSLFAAHNAFHHCEQCHHYSEPIPAAQLSDCTFHAFTFCSSMLGEEVQLHFIIPKSKESHFVFSQQGSHLESMRLPLLSDKESGMMKSPIFTPTTGRQEHGLLNIYHAMEGAEHLHILVVKQYEMPLYRKYWPNHILLVLPAMFNNSGVGAARFMIKELSYHNLELERNRQEEQGVKRQDVWPFIVMMDDSCVLWNAQQPGPDGKTEVMNVSLKSVLQHMEATPKISQYAVCGLRKWSSSLSSQAPTSPFSRCHLHDLILLNVDLTQNVQYDLNRFTCEEVDFNLRANSSGLLLCRFNQFSIMKKHIPIGGHKDFLIKPKLMRIETPVRVCASQYVCAPDSEQTLLAAPAQFLLEKFLQSCSHRLFPLALSNSANPVLSIDSYLNLGPEVQVCYVSSRPHSVNVDHQGVIFSGLLLYLCDSFVVSSLLKKFNFLKGATLCVICQDRSSLRQTIVRLELEDEWQFRLRDEFQTANCSEDRPLYFLTGRHI

Target ORF information:

RefSeq Version	XM_009298040.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio growth regulation by estrogen in breast cancer-like (greb1l), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298040.2

ORF Insert Sequence:

ATGGGAAATT CATACGCGGG GCAGCTGAAA TCTGCTCGCT TTGAGGAAGC TCTCCATAAC 
TCAATAGAGG CTTCATTAAG GTCAAGCGGT GGTGACCCAC AGCCGGTTTT CACACAGCTA 
TATCTCGAGC CTGATCAGTA TTCTGGCCAT GTAGAAGATA TTAAGCCCAA AATGGACCTA 
AGCCTGAGAT CCGACCCATC CACCCATGTG CTTGTGAAAT GCCACTCTTC AAATTCTGTA 
GAGGATATGG ATGACGAGGA CGATTCAGAC ACCAGCAGCC CACCATTGCC TTACCTGCAG 
GGACCCCCTC CTGATGGCTG CTGCACTGTT GATGGTTTCT GTCAAGCAGG AAAAGATCTG 
CGCCTGGTTT CCATGGCAAC AGAGTCTATC GAGGTTCCTG CAGGGTTTGA GCTGGTCGGT 
GCCAAATCGC CCAGCATCCC AGAGCACATC CTCGTCTGTG CAGTGGACAA GCGCTTCCTG 
CCAGATGAGA ATGGGAAAAA TGCACTTTTA GGGTTTTCAG GGAACTGCGT GGGTTGTGGT 
GAGAAAGGGT TCCGTTACTT CACCGAGTTC TCTAACCACA TTAACCTGAA GTTATCCACT 
CAGCCGAAGA AGCAGAAGCA CTTAAAGTAC TACCTGGTGA AGAACTCTCA GGGTGCTCTG 
TGCAAAGGAG CCCTCATTTG CTGGAAAGAT TGTAAGACAC GACCATTCTC AAACAGTGCT 
TCTTCATCCA AGCCTAGTTC CTCATCCTCA CTAAGCAGCA AAGAAAATGG AGACACAAAT 
GGCCACAGTC CATCTCCCTT CCCCCTCTCA GACTCGCCTC CTGCCCGCAT GCAATCTGGC 
TCATCCTCAG GGATTTTTGG ACCCCAGGAG CTGGGCTTCC TTAAACCATT GAACACACCG 
ACCCACGGGA CCAAGACATT ACCGATCGTT CCCACTGCCC TCCGAGTGAA TGGTCTAACT 
AATGGTCTCA GTATGGATGG ACGCTCCACT CTACTGAGCC CACCTCGTAC AAACCCACTC 
TCCACCCCTA GTCATGGCTA TAGAACCACA GAAACAGGAG ACAGTCCAGC ATCCACTGCT 
ATGTCGACAG GTCCACCAAA AAAGCGGCAT CGTAGTTGGC ATCCCACCAC TTTAGTGCCC 
ATCCCAGCCA CAGCTGTTCC TGTACCAGCC ATCCGACCGC TGACCTGCAG CTCTGGTCCT 
TTATTGTCAC TGTCCAATCA ACAGCCTGCA TCGGTGTCAG GGGTCATACA ACCACAGCCA 
ATCACAGCTG GAGAAACGGT CATCATTCCT GATAACCTTC TGAACTCTTA CGGGGTCCGG 
CCAGTCCTTC TCATTGGTCA AGGCACTTTA CCATATTTCT TTGGCAATGT GGGTGATCTG 
GTGGTGAGCC CCCTGCTGGT GAGCTGCTAC AAGGGCAGAG AGCTCAACGA GAAGACCCTG 
GCCAGCCTGG GCATGTCAGC AAACCAGTTA CTGACCACAG AGACCATGAT ACTGCTCACA 
CTGCAGTACC TCGCTCGACT GGGTACAGAG CAGATACCGT TACGGGAAGA GTTTGAGCAA 
ATCATGTTGA AGGCTATGCT GTGTGGTCCG ACTGGCCCTC CAGTGTCGCC TGCGCAGCTG 
CCCTGGTTAG CCCGAATGGA GGCCAGTGTG TCAGGGGGCA GTGTTCAGGT GCTGGTCACC 
CACGGCTCTC TTGGTGAGGG TATCTCTGAG TCTCTGCGCT CACTGAGTGA AACATCACCA 
CAGCAGCAGC AGTGTCTCCC CAACTATGTG CTCATTATCT GCACCTCCAA AAGTGGAGCC 
AATGAGTTCT GTGTGCTCGT GTTGGGTAAG TATCAGTCTC GAGCCTTGGC AGAGAGCATG 
CTGTCTACAA ACGAGTTTCT GAAGGAGATC AGTTATGAGC TCATCACAGG GAAAGTTAGT 
GTACTAGCCT CACACTTCCA GAGCACCTCT TTAGGTGACA ATATGGACAA ACAGCTGGTT 
CGTTATCAAC GTAAACGCAA AGACAGGGTG GTTCAACCTT TTCAGGGGCA TCTCACCGAG 
TACATCCACT CCCAGGAAGC TGCGACCATG ATACCAGAAT CAGGGCCAGA TCTGCTGAGC 
GATGACTTTC AGATACACCC TCCTCAGTTG AGCGTTGCAC GAAGCCTTTT GTCACAGGTT 
TGTGCTATTG CCGACTCAGG GAGCCAGAGC TTAGATCTGG GCCGATTCTG CAAAGTAGAC 
TTTCTGATTC TGGTGCCGCC CTCCCATGTT CTGGTTCACC AAACAGTCCA GCGAATACGA 
CAATCAGGCG TCCTAATAGA TCTCGGTATA GAGGACGTGT CATTAGCCAT GCAGAAGTCT 
GACAAGTATG TGGTGCGTCT GGACACAGAG GTCCACACAA AAATGGAGGC CTTTATGAGG 
AAGGTCAAGC AGAACCCTTA TACACTCTTC GTCCTCATTC ATGACAACTC GCACGTGGAC 
TTGACAAGTG CTCTTTCAGG GTCGGTTTGT CATGGGGAGC TGCAGGGATT GGCTGATCGC 
GTGGTGAACT GTCCAGAGGT TCTGGAAGCA ATAAACTTAC TGGTGCTGCA GGTCAGCTGC 
TTCCCGTTCA CCCTGCAGAG CCGGCAGTCA CGAATCAGTA CCCAGAATGA AGTGCACTGG 
CCCGACACTG AGAACCAGCA GGGTGAAGCT TCTCCTAAAG ACTTGATCTA CTTTGGGCTG 
AAGGACTACA GCAAGTCTCT GCAGTGGGGA GTGGCGAGTC CAATTCTGCG ATGTGATGAC 
GCCTTTGAGC GGATGGTTAA AACCCTGTTG GAGAGGCACC CACATTTGCA CAGTATGGTG 
ATCCGCAGCT ACCTGTTGAT CCAGCAGTAC ACAGAGGCTC TGATGGCCCT CACAGCGGCC 
CCTTCTTTGC GGGATCACGT GACCCCTCAG ACACTTGCCA TGGTGGAGGA TCTGCTGAGT 
GTGCCAGGCC GCAGCAAGCA TGGGTGCGGA CACATGCTGC TGGTGCGTGT GCCTTCTCTG 
CAGCTGGCAC GACTGGCACA GGAGCGGCTG GAGGAGGCTC GGGACAAGCT GGGGCTGCAG 
TACCGCTTTG CCGTGCTGCT AGGAAGCCCT GCTGCGGAGA TCAGCCTGCC GGTGCACTTC 
TGTGCCCGTC TGCGGGCTTG GAGGGGCTGT AAAAATGAGG AGTGGGTCCC ACATACATAT 
GAAGATCTGG AAGGACTTCC ATGCATTGTC ATCCTCACTG GAAAAGATCC TCTTGGAGAA 
ACCTTCCCGA GGTCTCTAAA GTACTGTGAC TTGCGGCTGA TTGACTCCAG CTACCTGACA 
CGCACAGCGC TGGAACAGGA AGTGGGTTTG GCCTGTTCAT ATGTGACACG TAGAGTAATC 
CCTAAGACCA AAACTGCCAC AAGCAGAGAA GAGAGACCGA GAGAAGGTGA AAGGAGCTCT 
GGAGAAACAG CAGAACACGA TGATCTCCCT ATGGAGCTGG AAAGACCACC CAGCAATGCC 
AGCGCTGCTA CTAGGACCTC CGGCTCCACC ACAGAAAATG GTGTCAGTTC CTCAAGTATA 
CTTGACAAGC CATCCTCGCA GAGTGACCCT TGTGGAAGCA GGACTATGAT GGACTCTTGC 
TCCTCTCCTG TTCGCTTCAA GCAGGAGTGC GACTCTCAGG CTCCCTCATC ATCCAGCACG 
TCCTCATTCT CCTCTGCTTC CTCCTCCTCT TCTTCTTCCT CGTCTCCGGC AGCTCAGAGA 
CCGAGTCAGT CTACACAGGC TCCACGTGAA TGTAACAGGA CACAGGTCTT TCCCCGGACG 
GCGGTGCTGT CTCGGGCTGC GTATACCCTG CTGGCTCCTG AAACTCTGGG TCATCCCAGT 
TCTGCCTCTC TGCTGCCACA TGCGGATGTG TCGTGGAGCA GTCCGCTCAG ACCACCGGTT 
CCTCATGGCC TTGGTGGAGC AGAGCAGTCC CTCTACTATC GTCAGTGGAC CACAGCCAGG 
CAACACCACG CTGATTATGA AGGCCCAGTA CCCCATCCCC ACCCAAGAAG ACTGCTTCTG 
AGCGGCCCAC CACAGGTTGG AAAAACAGGT GCATATCTGC AGTTCCTACG AATTCTGTTC 
CGTATGCTGA TTCGCCTTCT GGAGGTAGAT GTTTATGATG AAGAAGAGCT GGAGGAAGAT 
GTGCAAGACA AATCAAAGGT TCCCCCTTCT AGTGGGCCAC AGTGGCCTGA TGTGGAGGAC 
GTCCGCAAAC TAAGATTTGA CCTCTGCCCT CATGACTGCA AGTTCAAATA TTCAAGTCCA 
GTGTATGCCA ACAGGATGCC AAAAACACAG AGTGGGGTTA AAACCGAAAG ACTGGATACA 
GAAGCGGACC CACCAAAGAG AAACACAGTA TCTGTTAGAC TCAGCCTTTT TGCAGCCCAC 
AATGCATTTC ACCACTGTGA GCAGTGCCAC CATTACAGTG AACCTATTCC TGCTGCTCAG 
CTCTCTGACT GCACCTTCCA CGCTTTCACC TTTTGCTCAT CTATGCTGGG AGAGGAAGTC 
CAGCTGCACT TTATCATCCC TAAATCCAAA GAGTCTCATT TTGTGTTCAG CCAACAGGGC 
AGTCACCTAG AGAGCATGAG ACTGCCTCTG CTTTCCGACA AGGAGTCGGG CATGATGAAA 
AGCCCAATAT TCACCCCAAC CACGGGCCGC CAGGAGCACG GCCTTCTCAA CATCTACCAT 
GCCATGGAGG GAGCCGAGCA CCTGCACATC CTGGTGGTCA AACAGTATGA AATGCCACTC 
TATAGGAAAT ACTGGCCCAA CCACATCCTG CTGGTGCTGC CCGCCATGTT CAACAACTCA 
GGAGTCGGTG CGGCTCGATT CATGATCAAA GAACTGTCCT ATCACAACCT GGAGCTTGAG 
AGGAACCGAC AAGAGGAGCA AGGCGTCAAA CGGCAGGACG TGTGGCCCTT TATCGTAATG 
ATGGATGACT CCTGCGTCCT GTGGAACGCC CAGCAGCCTG GACCAGACGG CAAAACCGAG 
GTGATGAACG TGTCTCTGAA GAGCGTCTTG CAGCACATGG AAGCCACTCC AAAGATCTCC 
CAGTATGCAG TTTGTGGCCT GCGCAAATGG AGCAGTAGTT TGTCTTCTCA AGCTCCCACA 
AGCCCTTTCT CTCGCTGTCA TCTCCACGAC CTCATCTTGC TGAATGTTGA CCTCACACAG 
AACGTCCAGT ATGACCTCAA TCGGTTCACA TGTGAAGAGG TGGACTTCAA CCTGAGGGCA 
AACAGCAGTG GTCTTCTGCT CTGTCGCTTC AATCAATTCA GCATCATGAA GAAACACATT 
CCCATCGGAG GACATAAAGA CTTCCTCATC AAACCCAAGC TCATGAGAAT AGAGACGCCG 
GTGCGTGTGT GTGCGTCTCA ATATGTGTGC GCTCCGGACA GTGAACAAAC GTTGCTGGCT 
GCGCCAGCTC AGTTCCTGCT GGAGAAGTTT CTGCAGTCCT GTAGCCATCG GCTCTTTCCT 
CTGGCTCTCA GTAACAGTGC TAACCCTGTG TTGTCCATAG ACAGCTACCT CAACCTTGGA 
CCAGAGGTTC AGGTGTGTTA TGTGAGCTCG CGACCTCATT CGGTGAATGT AGATCATCAA 
GGTGTCATTT TCAGTGGCCT GCTGCTCTAC TTGTGTGACT CCTTTGTCGT TTCAAGCCTT 
CTAAAGAAAT TCAACTTCCT CAAAGGCGCC ACGCTGTGTG TGATCTGTCA GGACCGGAGC 
TCTCTGCGTC AGACTATTGT ACGTCTGGAG CTGGAGGATG AGTGGCAATT TCGCCTGCGA 
GATGAGTTTC AAACGGCCAA CTGCAGCGAG GACCGTCCGC TCTACTTCCT CACAGGACGA 
CATATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GREB1L ( XM_021466912.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021322587.1 Danio rerio growth regulation by estrogen in breast cancer-like (greb1l), transcript variant X3, mRNA.

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CloneID	ODa06787
Clone ID Related Accession (Same CDS sequence)	XM_001344416.8 , XM_009298040.1 , XM_009298040.2 , XM_021466912.1 , NM_001362307.1 , XM_001344416.7
Accession Version	XM_021466912.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	GREB1-like protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAATT CATACGCGGG GCAGCTGAAA TCTGCTCGCT TTGAGGAAGC TCTCCATAAC 
TCAATAGAGG CTTCATTAAG GTCAAGCGGT GGTGACCCAC AGCCGGTTTT CACACAGCTA 
TATCTCGAGC CTGATCAGTA TTCTGGCCAT GTAGAAGATA TTAAGCCCAA AATGGACCTA 
AGCCTGAGAT CCGACCCATC CACCCATGTG CTTGTGAAAT GCCACTCTTC AAATTCTGTA 
GAGGATATGG ATGACGAGGA CGATTCAGAC ACCAGCAGCC CACCATTGCC TTACCTGCAG 
GGACCCCCTC CTGATGGCTG CTGCACTGTT GATGGTTTCT GTCAAGCAGG AAAAGATCTG 
CGCCTGGTTT CCATGGCAAC AGAGTCTATC GAGGTTCCTG CAGGGTTTGA GCTGGTCGGT 
GCCAAATCGC CCAGCATCCC AGAGCACATC CTCGTCTGTG CAGTGGACAA GCGCTTCCTG 
CCAGATGAGA ATGGGAAAAA TGCACTTTTA GGGTTTTCAG GGAACTGCGT GGGTTGTGGT 
GAGAAAGGGT TCCGTTACTT CACCGAGTTC TCTAACCACA TTAACCTGAA GTTATCCACT 
CAGCCGAAGA AGCAGAAGCA CTTAAAGTAC TACCTGGTGA AGAACTCTCA GGGTGCTCTG 
TGCAAAGGAG CCCTCATTTG CTGGAAAGAT TGTAAGACAC GACCATTCTC AAACAGTGCT 
TCTTCATCCA AGCCTAGTTC CTCATCCTCA CTAAGCAGCA AAGAAAATGG AGACACAAAT 
GGCCACAGTC CATCTCCCTT CCCCCTCTCA GACTCGCCTC CTGCCCGCAT GCAATCTGGC 
TCATCCTCAG GGATTTTTGG ACCCCAGGAG CTGGGCTTCC TTAAACCATT GAACACACCG 
ACCCACGGGA CCAAGACATT ACCGATCGTT CCCACTGCCC TCCGAGTGAA TGGTCTAACT 
AATGGTCTCA GTATGGATGG ACGCTCCACT CTACTGAGCC CACCTCGTAC AAACCCACTC 
TCCACCCCTA GTCATGGCTA TAGAACCACA GAAACAGGAG ACAGTCCAGC ATCCACTGCT 
ATGTCGACAG GTCCACCAAA AAAGCGGCAT CGTAGTTGGC ATCCCACCAC TTTAGTGCCC 
ATCCCAGCCA CAGCTGTTCC TGTACCAGCC ATCCGACCGC TGACCTGCAG CTCTGGTCCT 
TTATTGTCAC TGTCCAATCA ACAGCCTGCA TCGGTGTCAG GGGTCATACA ACCACAGCCA 
ATCACAGCTG GAGAAACGGT CATCATTCCT GATAACCTTC TGAACTCTTA CGGGGTCCGG 
CCAGTCCTTC TCATTGGTCA AGGCACTTTA CCATATTTCT TTGGCAATGT GGGTGATCTG 
GTGGTGAGCC CCCTGCTGGT GAGCTGCTAC AAGGGCAGAG AGCTCAACGA GAAGACCCTG 
GCCAGCCTGG GCATGTCAGC AAACCAGTTA CTGACCACAG AGACCATGAT ACTGCTCACA 
CTGCAGTACC TCGCTCGACT GGGTACAGAG CAGATACCGT TACGGGAAGA GTTTGAGCAA 
ATCATGTTGA AGGCTATGCT GTGTGGTCCG ACTGGCCCTC CAGTGTCGCC TGCGCAGCTG 
CCCTGGTTAG CCCGAATGGA GGCCAGTGTG TCAGGGGGCA GTGTTCAGGT GCTGGTCACC 
CACGGCTCTC TTGGTGAGGG TATCTCTGAG TCTCTGCGCT CACTGAGTGA AACATCACCA 
CAGCAGCAGC AGTGTCTCCC CAACTATGTG CTCATTATCT GCACCTCCAA AAGTGGAGCC 
AATGAGTTCT GTGTGCTCGT GTTGGGTAAG TATCAGTCTC GAGCCTTGGC AGAGAGCATG 
CTGTCTACAA ACGAGTTTCT GAAGGAGATC AGTTATGAGC TCATCACAGG GAAAGTTAGT 
GTACTAGCCT CACACTTCCA GAGCACCTCT TTAGGTGACA ATATGGACAA ACAGCTGGTT 
CGTTATCAAC GTAAACGCAA AGACAGGGTG GTTCAACCTT TTCAGGGGCA TCTCACCGAG 
TACATCCACT CCCAGGAAGC TGCGACCATG ATACCAGAAT CAGGGCCAGA TCTGCTGAGC 
GATGACTTTC AGATACACCC TCCTCAGTTG AGCGTTGCAC GAAGCCTTTT GTCACAGGTT 
TGTGCTATTG CCGACTCAGG GAGCCAGAGC TTAGATCTGG GCCGATTCTG CAAAGTAGAC 
TTTCTGATTC TGGTGCCGCC CTCCCATGTT CTGGTTCACC AAACAGTCCA GCGAATACGA 
CAATCAGGCG TCCTAATAGA TCTCGGTATA GAGGACGTGT CATTAGCCAT GCAGAAGTCT 
GACAAGTATG TGGTGCGTCT GGACACAGAG GTCCACACAA AAATGGAGGC CTTTATGAGG 
AAGGTCAAGC AGAACCCTTA TACACTCTTC GTCCTCATTC ATGACAACTC GCACGTGGAC 
TTGACAAGTG CTCTTTCAGG GTCGGTTTGT CATGGGGAGC TGCAGGGATT GGCTGATCGC 
GTGGTGAACT GTCCAGAGGT TCTGGAAGCA ATAAACTTAC TGGTGCTGCA GGTCAGCTGC 
TTCCCGTTCA CCCTGCAGAG CCGGCAGTCA CGAATCAGTA CCCAGAATGA AGTGCACTGG 
CCCGACACTG AGAACCAGCA GGGTGAAGCT TCTCCTAAAG ACTTGATCTA CTTTGGGCTG 
AAGGACTACA GCAAGTCTCT GCAGTGGGGA GTGGCGAGTC CAATTCTGCG ATGTGATGAC 
GCCTTTGAGC GGATGGTTAA AACCCTGTTG GAGAGGCACC CACATTTGCA CAGTATGGTG 
ATCCGCAGCT ACCTGTTGAT CCAGCAGTAC ACAGAGGCTC TGATGGCCCT CACAGCGGCC 
CCTTCTTTGC GGGATCACGT GACCCCTCAG ACACTTGCCA TGGTGGAGGA TCTGCTGAGT 
GTGCCAGGCC GCAGCAAGCA TGGGTGCGGA CACATGCTGC TGGTGCGTGT GCCTTCTCTG 
CAGCTGGCAC GACTGGCACA GGAGCGGCTG GAGGAGGCTC GGGACAAGCT GGGGCTGCAG 
TACCGCTTTG CCGTGCTGCT AGGAAGCCCT GCTGCGGAGA TCAGCCTGCC GGTGCACTTC 
TGTGCCCGTC TGCGGGCTTG GAGGGGCTGT AAAAATGAGG AGTGGGTCCC ACATACATAT 
GAAGATCTGG AAGGACTTCC ATGCATTGTC ATCCTCACTG GAAAAGATCC TCTTGGAGAA 
ACCTTCCCGA GGTCTCTAAA GTACTGTGAC TTGCGGCTGA TTGACTCCAG CTACCTGACA 
CGCACAGCGC TGGAACAGGA AGTGGGTTTG GCCTGTTCAT ATGTGACACG TAGAGTAATC 
CCTAAGACCA AAACTGCCAC AAGCAGAGAA GAGAGACCGA GAGAAGGTGA AAGGAGCTCT 
GGAGAAACAG CAGAACACGA TGATCTCCCT ATGGAGCTGG AAAGACCACC CAGCAATGCC 
AGCGCTGCTA CTAGGACCTC CGGCTCCACC ACAGAAAATG GTGTCAGTTC CTCAAGTATA 
CTTGACAAGC CATCCTCGCA GAGTGACCCT TGTGGAAGCA GGACTATGAT GGACTCTTGC 
TCCTCTCCTG TTCGCTTCAA GCAGGAGTGC GACTCTCAGG CTCCCTCATC ATCCAGCACG 
TCCTCATTCT CCTCTGCTTC CTCCTCCTCT TCTTCTTCCT CGTCTCCGGC AGCTCAGAGA 
CCGAGTCAGT CTACACAGGC TCCACGTGAA TGTAACAGGA CACAGGTCTT TCCCCGGACG 
GCGGTGCTGT CTCGGGCTGC GTATACCCTG CTGGCTCCTG AAACTCTGGG TCATCCCAGT 
TCTGCCTCTC TGCTGCCACA TGCGGATGTG TCGTGGAGCA GTCCGCTCAG ACCACCGGTT 
CCTCATGGCC TTGGTGGAGC AGAGCAGTCC CTCTACTATC GTCAGTGGAC CACAGCCAGG 
CAACACCACG CTGATTATGA AGGCCCAGTA CCCCATCCCC ACCCAAGAAG ACTGCTTCTG 
AGCGGCCCAC CACAGGTTGG AAAAACAGGT GCATATCTGC AGTTCCTACG AATTCTGTTC 
CGTATGCTGA TTCGCCTTCT GGAGGTAGAT GTTTATGATG AAGAAGAGCT GGAGGAAGAT 
GTGCAAGACA AATCAAAGGT TCCCCCTTCT AGTGGGCCAC AGTGGCCTGA TGTGGAGGAC 
GTCCGCAAAC TAAGATTTGA CCTCTGCCCT CATGACTGCA AGTTCAAATA TTCAAGTCCA 
GTGTATGCCA ACAGGATGCC AAAAACACAG AGTGGGGTTA AAACCGAAAG ACTGGATACA 
GAAGCGGACC CACCAAAGAG AAACACAGTA TCTGTTAGAC TCAGCCTTTT TGCAGCCCAC 
AATGCATTTC ACCACTGTGA GCAGTGCCAC CATTACAGTG AACCTATTCC TGCTGCTCAG 
CTCTCTGACT GCACCTTCCA CGCTTTCACC TTTTGCTCAT CTATGCTGGG AGAGGAAGTC 
CAGCTGCACT TTATCATCCC TAAATCCAAA GAGTCTCATT TTGTGTTCAG CCAACAGGGC 
AGTCACCTAG AGAGCATGAG ACTGCCTCTG CTTTCCGACA AGGAGTCGGG CATGATGAAA 
AGCCCAATAT TCACCCCAAC CACGGGCCGC CAGGAGCACG GCCTTCTCAA CATCTACCAT 
GCCATGGAGG GAGCCGAGCA CCTGCACATC CTGGTGGTCA AACAGTATGA AATGCCACTC 
TATAGGAAAT ACTGGCCCAA CCACATCCTG CTGGTGCTGC CCGCCATGTT CAACAACTCA 
GGAGTCGGTG CGGCTCGATT CATGATCAAA GAACTGTCCT ATCACAACCT GGAGCTTGAG 
AGGAACCGAC AAGAGGAGCA AGGCGTCAAA CGGCAGGACG TGTGGCCCTT TATCGTAATG 
ATGGATGACT CCTGCGTCCT GTGGAACGCC CAGCAGCCTG GACCAGACGG CAAAACCGAG 
GTGATGAACG TGTCTCTGAA GAGCGTCTTG CAGCACATGG AAGCCACTCC AAAGATCTCC 
CAGTATGCAG TTTGTGGCCT GCGCAAATGG AGCAGTAGTT TGTCTTCTCA AGCTCCCACA 
AGCCCTTTCT CTCGCTGTCA TCTCCACGAC CTCATCTTGC TGAATGTTGA CCTCACACAG 
AACGTCCAGT ATGACCTCAA TCGGTTCACA TGTGAAGAGG TGGACTTCAA CCTGAGGGCA 
AACAGCAGTG GTCTTCTGCT CTGTCGCTTC AATCAATTCA GCATCATGAA GAAACACATT 
CCCATCGGAG GACATAAAGA CTTCCTCATC AAACCCAAGC TCATGAGAAT AGAGACGCCG 
GTGCGTGTGT GTGCGTCTCA ATATGTGTGC GCTCCGGACA GTGAACAAAC GTTGCTGGCT 
GCGCCAGCTC AGTTCCTGCT GGAGAAGTTT CTGCAGTCCT GTAGCCATCG GCTCTTTCCT 
CTGGCTCTCA GTAACAGTGC TAACCCTGTG TTGTCCATAG ACAGCTACCT CAACCTTGGA 
CCAGAGGTTC AGGTGTGTTA TGTGAGCTCG CGACCTCATT CGGTGAATGT AGATCATCAA 
GGTGTCATTT TCAGTGGCCT GCTGCTCTAC TTGTGTGACT CCTTTGTCGT TTCAAGCCTT 
CTAAAGAAAT TCAACTTCCT CAAAGGCGCC ACGCTGTGTG TGATCTGTCA GGACCGGAGC 
TCTCTGCGTC AGACTATTGT ACGTCTGGAG CTGGAGGATG AGTGGCAATT TCGCCTGCGA 
GATGAGTTTC AAACGGCCAA CTGCAGCGAG GACCGTCCGC TCTACTTCCT CACAGGACGA 
CATATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021322587.1
CDS	288..6116
Translation	MGNSYAGQLKSARFEEALHNSIEASLRSSGGDPQPVFTQLYLEPDQYSGHVEDIKPKMDLSLRSDPSTHVLVKCHSSNSVEDMDDEDDSDTSSPPLPYLQGPPPDGCCTVDGFCQAGKDLRLVSMATESIEVPAGFELVGAKSPSIPEHILVCAVDKRFLPDENGKNALLGFSGNCVGCGEKGFRYFTEFSNHINLKLSTQPKKQKHLKYYLVKNSQGALCKGALICWKDCKTRPFSNSASSSKPSSSSSLSSKENGDTNGHSPSPFPLSDSPPARMQSGSSSGIFGPQELGFLKPLNTPTHGTKTLPIVPTALRVNGLTNGLSMDGRSTLLSPPRTNPLSTPSHGYRTTETGDSPASTAMSTGPPKKRHRSWHPTTLVPIPATAVPVPAIRPLTCSSGPLLSLSNQQPASVSGVIQPQPITAGETVIIPDNLLNSYGVRPVLLIGQGTLPYFFGNVGDLVVSPLLVSCYKGRELNEKTLASLGMSANQLLTTETMILLTLQYLARLGTEQIPLREEFEQIMLKAMLCGPTGPPVSPAQLPWLARMEASVSGGSVQVLVTHGSLGEGISESLRSLSETSPQQQQCLPNYVLIICTSKSGANEFCVLVLGKYQSRALAESMLSTNEFLKEISYELITGKVSVLASHFQSTSLGDNMDKQLVRYQRKRKDRVVQPFQGHLTEYIHSQEAATMIPESGPDLLSDDFQIHPPQLSVARSLLSQVCAIADSGSQSLDLGRFCKVDFLILVPPSHVLVHQTVQRIRQSGVLIDLGIEDVSLAMQKSDKYVVRLDTEVHTKMEAFMRKVKQNPYTLFVLIHDNSHVDLTSALSGSVCHGELQGLADRVVNCPEVLEAINLLVLQVSCFPFTLQSRQSRISTQNEVHWPDTENQQGEASPKDLIYFGLKDYSKSLQWGVASPILRCDDAFERMVKTLLERHPHLHSMVIRSYLLIQQYTEALMALTAAPSLRDHVTPQTLAMVEDLLSVPGRSKHGCGHMLLVRVPSLQLARLAQERLEEARDKLGLQYRFAVLLGSPAAEISLPVHFCARLRAWRGCKNEEWVPHTYEDLEGLPCIVILTGKDPLGETFPRSLKYCDLRLIDSSYLTRTALEQEVGLACSYVTRRVIPKTKTATSREERPREGERSSGETAEHDDLPMELERPPSNASAATRTSGSTTENGVSSSSILDKPSSQSDPCGSRTMMDSCSSPVRFKQECDSQAPSSSSTSSFSSASSSSSSSSSPAAQRPSQSTQAPRECNRTQVFPRTAVLSRAAYTLLAPETLGHPSSASLLPHADVSWSSPLRPPVPHGLGGAEQSLYYRQWTTARQHHADYEGPVPHPHPRRLLLSGPPQVGKTGAYLQFLRILFRMLIRLLEVDVYDEEELEEDVQDKSKVPPSSGPQWPDVEDVRKLRFDLCPHDCKFKYSSPVYANRMPKTQSGVKTERLDTEADPPKRNTVSVRLSLFAAHNAFHHCEQCHHYSEPIPAAQLSDCTFHAFTFCSSMLGEEVQLHFIIPKSKESHFVFSQQGSHLESMRLPLLSDKESGMMKSPIFTPTTGRQEHGLLNIYHAMEGAEHLHILVVKQYEMPLYRKYWPNHILLVLPAMFNNSGVGAARFMIKELSYHNLELERNRQEEQGVKRQDVWPFIVMMDDSCVLWNAQQPGPDGKTEVMNVSLKSVLQHMEATPKISQYAVCGLRKWSSSLSSQAPTSPFSRCHLHDLILLNVDLTQNVQYDLNRFTCEEVDFNLRANSSGLLLCRFNQFSIMKKHIPIGGHKDFLIKPKLMRIETPVRVCASQYVCAPDSEQTLLAAPAQFLLEKFLQSCSHRLFPLALSNSANPVLSIDSYLNLGPEVQVCYVSSRPHSVNVDHQGVIFSGLLLYLCDSFVVSSLLKKFNFLKGATLCVICQDRSSLRQTIVRLELEDEWQFRLRDEFQTANCSEDRPLYFLTGRHI

Target ORF information:

RefSeq Version	XM_021466912.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio growth regulation by estrogen in breast cancer-like (greb1l), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021466912.1

ORF Insert Sequence:

ATGGGAAATT CATACGCGGG GCAGCTGAAA TCTGCTCGCT TTGAGGAAGC TCTCCATAAC 
TCAATAGAGG CTTCATTAAG GTCAAGCGGT GGTGACCCAC AGCCGGTTTT CACACAGCTA 
TATCTCGAGC CTGATCAGTA TTCTGGCCAT GTAGAAGATA TTAAGCCCAA AATGGACCTA 
AGCCTGAGAT CCGACCCATC CACCCATGTG CTTGTGAAAT GCCACTCTTC AAATTCTGTA 
GAGGATATGG ATGACGAGGA CGATTCAGAC ACCAGCAGCC CACCATTGCC TTACCTGCAG 
GGACCCCCTC CTGATGGCTG CTGCACTGTT GATGGTTTCT GTCAAGCAGG AAAAGATCTG 
CGCCTGGTTT CCATGGCAAC AGAGTCTATC GAGGTTCCTG CAGGGTTTGA GCTGGTCGGT 
GCCAAATCGC CCAGCATCCC AGAGCACATC CTCGTCTGTG CAGTGGACAA GCGCTTCCTG 
CCAGATGAGA ATGGGAAAAA TGCACTTTTA GGGTTTTCAG GGAACTGCGT GGGTTGTGGT 
GAGAAAGGGT TCCGTTACTT CACCGAGTTC TCTAACCACA TTAACCTGAA GTTATCCACT 
CAGCCGAAGA AGCAGAAGCA CTTAAAGTAC TACCTGGTGA AGAACTCTCA GGGTGCTCTG 
TGCAAAGGAG CCCTCATTTG CTGGAAAGAT TGTAAGACAC GACCATTCTC AAACAGTGCT 
TCTTCATCCA AGCCTAGTTC CTCATCCTCA CTAAGCAGCA AAGAAAATGG AGACACAAAT 
GGCCACAGTC CATCTCCCTT CCCCCTCTCA GACTCGCCTC CTGCCCGCAT GCAATCTGGC 
TCATCCTCAG GGATTTTTGG ACCCCAGGAG CTGGGCTTCC TTAAACCATT GAACACACCG 
ACCCACGGGA CCAAGACATT ACCGATCGTT CCCACTGCCC TCCGAGTGAA TGGTCTAACT 
AATGGTCTCA GTATGGATGG ACGCTCCACT CTACTGAGCC CACCTCGTAC AAACCCACTC 
TCCACCCCTA GTCATGGCTA TAGAACCACA GAAACAGGAG ACAGTCCAGC ATCCACTGCT 
ATGTCGACAG GTCCACCAAA AAAGCGGCAT CGTAGTTGGC ATCCCACCAC TTTAGTGCCC 
ATCCCAGCCA CAGCTGTTCC TGTACCAGCC ATCCGACCGC TGACCTGCAG CTCTGGTCCT 
TTATTGTCAC TGTCCAATCA ACAGCCTGCA TCGGTGTCAG GGGTCATACA ACCACAGCCA 
ATCACAGCTG GAGAAACGGT CATCATTCCT GATAACCTTC TGAACTCTTA CGGGGTCCGG 
CCAGTCCTTC TCATTGGTCA AGGCACTTTA CCATATTTCT TTGGCAATGT GGGTGATCTG 
GTGGTGAGCC CCCTGCTGGT GAGCTGCTAC AAGGGCAGAG AGCTCAACGA GAAGACCCTG 
GCCAGCCTGG GCATGTCAGC AAACCAGTTA CTGACCACAG AGACCATGAT ACTGCTCACA 
CTGCAGTACC TCGCTCGACT GGGTACAGAG CAGATACCGT TACGGGAAGA GTTTGAGCAA 
ATCATGTTGA AGGCTATGCT GTGTGGTCCG ACTGGCCCTC CAGTGTCGCC TGCGCAGCTG 
CCCTGGTTAG CCCGAATGGA GGCCAGTGTG TCAGGGGGCA GTGTTCAGGT GCTGGTCACC 
CACGGCTCTC TTGGTGAGGG TATCTCTGAG TCTCTGCGCT CACTGAGTGA AACATCACCA 
CAGCAGCAGC AGTGTCTCCC CAACTATGTG CTCATTATCT GCACCTCCAA AAGTGGAGCC 
AATGAGTTCT GTGTGCTCGT GTTGGGTAAG TATCAGTCTC GAGCCTTGGC AGAGAGCATG 
CTGTCTACAA ACGAGTTTCT GAAGGAGATC AGTTATGAGC TCATCACAGG GAAAGTTAGT 
GTACTAGCCT CACACTTCCA GAGCACCTCT TTAGGTGACA ATATGGACAA ACAGCTGGTT 
CGTTATCAAC GTAAACGCAA AGACAGGGTG GTTCAACCTT TTCAGGGGCA TCTCACCGAG 
TACATCCACT CCCAGGAAGC TGCGACCATG ATACCAGAAT CAGGGCCAGA TCTGCTGAGC 
GATGACTTTC AGATACACCC TCCTCAGTTG AGCGTTGCAC GAAGCCTTTT GTCACAGGTT 
TGTGCTATTG CCGACTCAGG GAGCCAGAGC TTAGATCTGG GCCGATTCTG CAAAGTAGAC 
TTTCTGATTC TGGTGCCGCC CTCCCATGTT CTGGTTCACC AAACAGTCCA GCGAATACGA 
CAATCAGGCG TCCTAATAGA TCTCGGTATA GAGGACGTGT CATTAGCCAT GCAGAAGTCT 
GACAAGTATG TGGTGCGTCT GGACACAGAG GTCCACACAA AAATGGAGGC CTTTATGAGG 
AAGGTCAAGC AGAACCCTTA TACACTCTTC GTCCTCATTC ATGACAACTC GCACGTGGAC 
TTGACAAGTG CTCTTTCAGG GTCGGTTTGT CATGGGGAGC TGCAGGGATT GGCTGATCGC 
GTGGTGAACT GTCCAGAGGT TCTGGAAGCA ATAAACTTAC TGGTGCTGCA GGTCAGCTGC 
TTCCCGTTCA CCCTGCAGAG CCGGCAGTCA CGAATCAGTA CCCAGAATGA AGTGCACTGG 
CCCGACACTG AGAACCAGCA GGGTGAAGCT TCTCCTAAAG ACTTGATCTA CTTTGGGCTG 
AAGGACTACA GCAAGTCTCT GCAGTGGGGA GTGGCGAGTC CAATTCTGCG ATGTGATGAC 
GCCTTTGAGC GGATGGTTAA AACCCTGTTG GAGAGGCACC CACATTTGCA CAGTATGGTG 
ATCCGCAGCT ACCTGTTGAT CCAGCAGTAC ACAGAGGCTC TGATGGCCCT CACAGCGGCC 
CCTTCTTTGC GGGATCACGT GACCCCTCAG ACACTTGCCA TGGTGGAGGA TCTGCTGAGT 
GTGCCAGGCC GCAGCAAGCA TGGGTGCGGA CACATGCTGC TGGTGCGTGT GCCTTCTCTG 
CAGCTGGCAC GACTGGCACA GGAGCGGCTG GAGGAGGCTC GGGACAAGCT GGGGCTGCAG 
TACCGCTTTG CCGTGCTGCT AGGAAGCCCT GCTGCGGAGA TCAGCCTGCC GGTGCACTTC 
TGTGCCCGTC TGCGGGCTTG GAGGGGCTGT AAAAATGAGG AGTGGGTCCC ACATACATAT 
GAAGATCTGG AAGGACTTCC ATGCATTGTC ATCCTCACTG GAAAAGATCC TCTTGGAGAA 
ACCTTCCCGA GGTCTCTAAA GTACTGTGAC TTGCGGCTGA TTGACTCCAG CTACCTGACA 
CGCACAGCGC TGGAACAGGA AGTGGGTTTG GCCTGTTCAT ATGTGACACG TAGAGTAATC 
CCTAAGACCA AAACTGCCAC AAGCAGAGAA GAGAGACCGA GAGAAGGTGA AAGGAGCTCT 
GGAGAAACAG CAGAACACGA TGATCTCCCT ATGGAGCTGG AAAGACCACC CAGCAATGCC 
AGCGCTGCTA CTAGGACCTC CGGCTCCACC ACAGAAAATG GTGTCAGTTC CTCAAGTATA 
CTTGACAAGC CATCCTCGCA GAGTGACCCT TGTGGAAGCA GGACTATGAT GGACTCTTGC 
TCCTCTCCTG TTCGCTTCAA GCAGGAGTGC GACTCTCAGG CTCCCTCATC ATCCAGCACG 
TCCTCATTCT CCTCTGCTTC CTCCTCCTCT TCTTCTTCCT CGTCTCCGGC AGCTCAGAGA 
CCGAGTCAGT CTACACAGGC TCCACGTGAA TGTAACAGGA CACAGGTCTT TCCCCGGACG 
GCGGTGCTGT CTCGGGCTGC GTATACCCTG CTGGCTCCTG AAACTCTGGG TCATCCCAGT 
TCTGCCTCTC TGCTGCCACA TGCGGATGTG TCGTGGAGCA GTCCGCTCAG ACCACCGGTT 
CCTCATGGCC TTGGTGGAGC AGAGCAGTCC CTCTACTATC GTCAGTGGAC CACAGCCAGG 
CAACACCACG CTGATTATGA AGGCCCAGTA CCCCATCCCC ACCCAAGAAG ACTGCTTCTG 
AGCGGCCCAC CACAGGTTGG AAAAACAGGT GCATATCTGC AGTTCCTACG AATTCTGTTC 
CGTATGCTGA TTCGCCTTCT GGAGGTAGAT GTTTATGATG AAGAAGAGCT GGAGGAAGAT 
GTGCAAGACA AATCAAAGGT TCCCCCTTCT AGTGGGCCAC AGTGGCCTGA TGTGGAGGAC 
GTCCGCAAAC TAAGATTTGA CCTCTGCCCT CATGACTGCA AGTTCAAATA TTCAAGTCCA 
GTGTATGCCA ACAGGATGCC AAAAACACAG AGTGGGGTTA AAACCGAAAG ACTGGATACA 
GAAGCGGACC CACCAAAGAG AAACACAGTA TCTGTTAGAC TCAGCCTTTT TGCAGCCCAC 
AATGCATTTC ACCACTGTGA GCAGTGCCAC CATTACAGTG AACCTATTCC TGCTGCTCAG 
CTCTCTGACT GCACCTTCCA CGCTTTCACC TTTTGCTCAT CTATGCTGGG AGAGGAAGTC 
CAGCTGCACT TTATCATCCC TAAATCCAAA GAGTCTCATT TTGTGTTCAG CCAACAGGGC 
AGTCACCTAG AGAGCATGAG ACTGCCTCTG CTTTCCGACA AGGAGTCGGG CATGATGAAA 
AGCCCAATAT TCACCCCAAC CACGGGCCGC CAGGAGCACG GCCTTCTCAA CATCTACCAT 
GCCATGGAGG GAGCCGAGCA CCTGCACATC CTGGTGGTCA AACAGTATGA AATGCCACTC 
TATAGGAAAT ACTGGCCCAA CCACATCCTG CTGGTGCTGC CCGCCATGTT CAACAACTCA 
GGAGTCGGTG CGGCTCGATT CATGATCAAA GAACTGTCCT ATCACAACCT GGAGCTTGAG 
AGGAACCGAC AAGAGGAGCA AGGCGTCAAA CGGCAGGACG TGTGGCCCTT TATCGTAATG 
ATGGATGACT CCTGCGTCCT GTGGAACGCC CAGCAGCCTG GACCAGACGG CAAAACCGAG 
GTGATGAACG TGTCTCTGAA GAGCGTCTTG CAGCACATGG AAGCCACTCC AAAGATCTCC 
CAGTATGCAG TTTGTGGCCT GCGCAAATGG AGCAGTAGTT TGTCTTCTCA AGCTCCCACA 
AGCCCTTTCT CTCGCTGTCA TCTCCACGAC CTCATCTTGC TGAATGTTGA CCTCACACAG 
AACGTCCAGT ATGACCTCAA TCGGTTCACA TGTGAAGAGG TGGACTTCAA CCTGAGGGCA 
AACAGCAGTG GTCTTCTGCT CTGTCGCTTC AATCAATTCA GCATCATGAA GAAACACATT 
CCCATCGGAG GACATAAAGA CTTCCTCATC AAACCCAAGC TCATGAGAAT AGAGACGCCG 
GTGCGTGTGT GTGCGTCTCA ATATGTGTGC GCTCCGGACA GTGAACAAAC GTTGCTGGCT 
GCGCCAGCTC AGTTCCTGCT GGAGAAGTTT CTGCAGTCCT GTAGCCATCG GCTCTTTCCT 
CTGGCTCTCA GTAACAGTGC TAACCCTGTG TTGTCCATAG ACAGCTACCT CAACCTTGGA 
CCAGAGGTTC AGGTGTGTTA TGTGAGCTCG CGACCTCATT CGGTGAATGT AGATCATCAA 
GGTGTCATTT TCAGTGGCCT GCTGCTCTAC TTGTGTGACT CCTTTGTCGT TTCAAGCCTT 
CTAAAGAAAT TCAACTTCCT CAAAGGCGCC ACGCTGTGTG TGATCTGTCA GGACCGGAGC 
TCTCTGCGTC AGACTATTGT ACGTCTGGAG CTGGAGGATG AGTGGCAATT TCGCCTGCGA 
GATGAGTTTC AAACGGCCAA CTGCAGCGAG GACCGTCCGC TCTACTTCCT CACAGGACGA 
CATATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GREB1L ( XM_001344416.7 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_001344452.4 Danio rerio growth regulation by estrogen in breast cancer-like (greb1l), transcript variant X1, mRNA.

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CloneID	ODa06787
Clone ID Related Accession (Same CDS sequence)	XM_001344416.8 , XM_009298040.1 , XM_009298040.2 , XM_021466912.1 , NM_001362307.1 , XM_001344416.7
Accession Version	XM_001344416.7	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	GREB1-like protein
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001344416.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAATT CATACGCGGG GCAGCTGAAA TCTGCTCGCT TTGAGGAAGC TCTCCATAAC 
TCAATAGAGG CTTCATTAAG GTCAAGCGGT GGTGACCCAC AGCCGGTTTT CACACAGCTA 
TATCTCGAGC CTGATCAGTA TTCTGGCCAT GTAGAAGATA TTAAGCCCAA AATGGACCTA 
AGCCTGAGAT CCGACCCATC CACCCATGTG CTTGTGAAAT GCCACTCTTC AAATTCTGTA 
GAGGATATGG ATGACGAGGA CGATTCAGAC ACCAGCAGCC CACCATTGCC TTACCTGCAG 
GGACCCCCTC CTGATGGCTG CTGCACTGTT GATGGTTTCT GTCAAGCAGG AAAAGATCTG 
CGCCTGGTTT CCATGGCAAC AGAGTCTATC GAGGTTCCTG CAGGGTTTGA GCTGGTCGGT 
GCCAAATCGC CCAGCATCCC AGAGCACATC CTCGTCTGTG CAGTGGACAA GCGCTTCCTG 
CCAGATGAGA ATGGGAAAAA TGCACTTTTA GGGTTTTCAG GGAACTGCGT GGGTTGTGGT 
GAGAAAGGGT TCCGTTACTT CACCGAGTTC TCTAACCACA TTAACCTGAA GTTATCCACT 
CAGCCGAAGA AGCAGAAGCA CTTAAAGTAC TACCTGGTGA AGAACTCTCA GGGTGCTCTG 
TGCAAAGGAG CCCTCATTTG CTGGAAAGAT TGTAAGACAC GACCATTCTC AAACAGTGCT 
TCTTCATCCA AGCCTAGTTC CTCATCCTCA CTAAGCAGCA AAGAAAATGG AGACACAAAT 
GGCCACAGTC CATCTCCCTT CCCCCTCTCA GACTCGCCTC CTGCCCGCAT GCAATCTGGC 
TCATCCTCAG GGATTTTTGG ACCCCAGGAG CTGGGCTTCC TTAAACCATT GAACACACCG 
ACCCACGGGA CCAAGACATT ACCGATCGTT CCCACTGCCC TCCGAGTGAA TGGTCTAACT 
AATGGTCTCA GTATGGATGG ACGCTCCACT CTACTGAGCC CACCTCGTAC AAACCCACTC 
TCCACCCCTA GTCATGGCTA TAGAACCACA GAAACAGGAG ACAGTCCAGC ATCCACTGCT 
ATGTCGACAG GTCCACCAAA AAAGCGGCAT CGTAGTTGGC ATCCCACCAC TTTAGTGCCC 
ATCCCAGCCA CAGCTGTTCC TGTACCAGCC ATCCGACCGC TGACCTGCAG CTCTGGTCCT 
TTATTGTCAC TGTCCAATCA ACAGCCTGCA TCGGTGTCAG GGGTCATACA ACCACAGCCA 
ATCACAGCTG GAGAAACGGT CATCATTCCT GATAACCTTC TGAACTCTTA CGGGGTCCGG 
CCAGTCCTTC TCATTGGTCA AGGCACTTTA CCATATTTCT TTGGCAATGT GGGTGATCTG 
GTGGTGAGCC CCCTGCTGGT GAGCTGCTAC AAGGGCAGAG AGCTCAACGA GAAGACCCTG 
GCCAGCCTGG GCATGTCAGC AAACCAGTTA CTGACCACAG AGACCATGAT ACTGCTCACA 
CTGCAGTACC TCGCTCGACT GGGTACAGAG CAGATACCGT TACGGGAAGA GTTTGAGCAA 
ATCATGTTGA AGGCTATGCT GTGTGGTCCG ACTGGCCCTC CAGTGTCGCC TGCGCAGCTG 
CCCTGGTTAG CCCGAATGGA GGCCAGTGTG TCAGGGGGCA GTGTTCAGGT GCTGGTCACC 
CACGGCTCTC TTGGTGAGGG TATCTCTGAG TCTCTGCGCT CACTGAGTGA AACATCACCA 
CAGCAGCAGC AGTGTCTCCC CAACTATGTG CTCATTATCT GCACCTCCAA AAGTGGAGCC 
AATGAGTTCT GTGTGCTCGT GTTGGGTAAG TATCAGTCTC GAGCCTTGGC AGAGAGCATG 
CTGTCTACAA ACGAGTTTCT GAAGGAGATC AGTTATGAGC TCATCACAGG GAAAGTTAGT 
GTACTAGCCT CACACTTCCA GAGCACCTCT TTAGGTGACA ATATGGACAA ACAGCTGGTT 
CGTTATCAAC GTAAACGCAA AGACAGGGTG GTTCAACCTT TTCAGGGGCA TCTCACCGAG 
TACATCCACT CCCAGGAAGC TGCGACCATG ATACCAGAAT CAGGGCCAGA TCTGCTGAGC 
GATGACTTTC AGATACACCC TCCTCAGTTG AGCGTTGCAC GAAGCCTTTT GTCACAGGTT 
TGTGCTATTG CCGACTCAGG GAGCCAGAGC TTAGATCTGG GCCGATTCTG CAAAGTAGAC 
TTTCTGATTC TGGTGCCGCC CTCCCATGTT CTGGTTCACC AAACAGTCCA GCGAATACGA 
CAATCAGGCG TCCTAATAGA TCTCGGTATA GAGGACGTGT CATTAGCCAT GCAGAAGTCT 
GACAAGTATG TGGTGCGTCT GGACACAGAG GTCCACACAA AAATGGAGGC CTTTATGAGG 
AAGGTCAAGC AGAACCCTTA TACACTCTTC GTCCTCATTC ATGACAACTC GCACGTGGAC 
TTGACAAGTG CTCTTTCAGG GTCGGTTTGT CATGGGGAGC TGCAGGGATT GGCTGATCGC 
GTGGTGAACT GTCCAGAGGT TCTGGAAGCA ATAAACTTAC TGGTGCTGCA GGTCAGCTGC 
TTCCCGTTCA CCCTGCAGAG CCGGCAGTCA CGAATCAGTA CCCAGAATGA AGTGCACTGG 
CCCGACACTG AGAACCAGCA GGGTGAAGCT TCTCCTAAAG ACTTGATCTA CTTTGGGCTG 
AAGGACTACA GCAAGTCTCT GCAGTGGGGA GTGGCGAGTC CAATTCTGCG ATGTGATGAC 
GCCTTTGAGC GGATGGTTAA AACCCTGTTG GAGAGGCACC CACATTTGCA CAGTATGGTG 
ATCCGCAGCT ACCTGTTGAT CCAGCAGTAC ACAGAGGCTC TGATGGCCCT CACAGCGGCC 
CCTTCTTTGC GGGATCACGT GACCCCTCAG ACACTTGCCA TGGTGGAGGA TCTGCTGAGT 
GTGCCAGGCC GCAGCAAGCA TGGGTGCGGA CACATGCTGC TGGTGCGTGT GCCTTCTCTG 
CAGCTGGCAC GACTGGCACA GGAGCGGCTG GAGGAGGCTC GGGACAAGCT GGGGCTGCAG 
TACCGCTTTG CCGTGCTGCT AGGAAGCCCT GCTGCGGAGA TCAGCCTGCC GGTGCACTTC 
TGTGCCCGTC TGCGGGCTTG GAGGGGCTGT AAAAATGAGG AGTGGGTCCC ACATACATAT 
GAAGATCTGG AAGGACTTCC ATGCATTGTC ATCCTCACTG GAAAAGATCC TCTTGGAGAA 
ACCTTCCCGA GGTCTCTAAA GTACTGTGAC TTGCGGCTGA TTGACTCCAG CTACCTGACA 
CGCACAGCGC TGGAACAGGA AGTGGGTTTG GCCTGTTCAT ATGTGACACG TAGAGTAATC 
CCTAAGACCA AAACTGCCAC AAGCAGAGAA GAGAGACCGA GAGAAGGTGA AAGGAGCTCT 
GGAGAAACAG CAGAACACGA TGATCTCCCT ATGGAGCTGG AAAGACCACC CAGCAATGCC 
AGCGCTGCTA CTAGGACCTC CGGCTCCACC ACAGAAAATG GTGTCAGTTC CTCAAGTATA 
CTTGACAAGC CATCCTCGCA GAGTGACCCT TGTGGAAGCA GGACTATGAT GGACTCTTGC 
TCCTCTCCTG TTCGCTTCAA GCAGGAGTGC GACTCTCAGG CTCCCTCATC ATCCAGCACG 
TCCTCATTCT CCTCTGCTTC CTCCTCCTCT TCTTCTTCCT CGTCTCCGGC AGCTCAGAGA 
CCGAGTCAGT CTACACAGGC TCCACGTGAA TGTAACAGGA CACAGGTCTT TCCCCGGACG 
GCGGTGCTGT CTCGGGCTGC GTATACCCTG CTGGCTCCTG AAACTCTGGG TCATCCCAGT 
TCTGCCTCTC TGCTGCCACA TGCGGATGTG TCGTGGAGCA GTCCGCTCAG ACCACCGGTT 
CCTCATGGCC TTGGTGGAGC AGAGCAGTCC CTCTACTATC GTCAGTGGAC CACAGCCAGG 
CAACACCACG CTGATTATGA AGGCCCAGTA CCCCATCCCC ACCCAAGAAG ACTGCTTCTG 
AGCGGCCCAC CACAGGTTGG AAAAACAGGT GCATATCTGC AGTTCCTACG AATTCTGTTC 
CGTATGCTGA TTCGCCTTCT GGAGGTAGAT GTTTATGATG AAGAAGAGCT GGAGGAAGAT 
GTGCAAGACA AATCAAAGGT TCCCCCTTCT AGTGGGCCAC AGTGGCCTGA TGTGGAGGAC 
GTCCGCAAAC TAAGATTTGA CCTCTGCCCT CATGACTGCA AGTTCAAATA TTCAAGTCCA 
GTGTATGCCA ACAGGATGCC AAAAACACAG AGTGGGGTTA AAACCGAAAG ACTGGATACA 
GAAGCGGACC CACCAAAGAG AAACACAGTA TCTGTTAGAC TCAGCCTTTT TGCAGCCCAC 
AATGCATTTC ACCACTGTGA GCAGTGCCAC CATTACAGTG AACCTATTCC TGCTGCTCAG 
CTCTCTGACT GCACCTTCCA CGCTTTCACC TTTTGCTCAT CTATGCTGGG AGAGGAAGTC 
CAGCTGCACT TTATCATCCC TAAATCCAAA GAGTCTCATT TTGTGTTCAG CCAACAGGGC 
AGTCACCTAG AGAGCATGAG ACTGCCTCTG CTTTCCGACA AGGAGTCGGG CATGATGAAA 
AGCCCAATAT TCACCCCAAC CACGGGCCGC CAGGAGCACG GCCTTCTCAA CATCTACCAT 
GCCATGGAGG GAGCCGAGCA CCTGCACATC CTGGTGGTCA AACAGTATGA AATGCCACTC 
TATAGGAAAT ACTGGCCCAA CCACATCCTG CTGGTGCTGC CCGCCATGTT CAACAACTCA 
GGAGTCGGTG CGGCTCGATT CATGATCAAA GAACTGTCCT ATCACAACCT GGAGCTTGAG 
AGGAACCGAC AAGAGGAGCA AGGCGTCAAA CGGCAGGACG TGTGGCCCTT TATCGTAATG 
ATGGATGACT CCTGCGTCCT GTGGAACGCC CAGCAGCCTG GACCAGACGG CAAAACCGAG 
GTGATGAACG TGTCTCTGAA GAGCGTCTTG CAGCACATGG AAGCCACTCC AAAGATCTCC 
CAGTATGCAG TTTGTGGCCT GCGCAAATGG AGCAGTAGTT TGTCTTCTCA AGCTCCCACA 
AGCCCTTTCT CTCGCTGTCA TCTCCACGAC CTCATCTTGC TGAATGTTGA CCTCACACAG 
AACGTCCAGT ATGACCTCAA TCGGTTCACA TGTGAAGAGG TGGACTTCAA CCTGAGGGCA 
AACAGCAGTG GTCTTCTGCT CTGTCGCTTC AATCAATTCA GCATCATGAA GAAACACATT 
CCCATCGGAG GACATAAAGA CTTCCTCATC AAACCCAAGC TCATGAGAAT AGAGACGCCG 
GTGCGTGTGT GTGCGTCTCA ATATGTGTGC GCTCCGGACA GTGAACAAAC GTTGCTGGCT 
GCGCCAGCTC AGTTCCTGCT GGAGAAGTTT CTGCAGTCCT GTAGCCATCG GCTCTTTCCT 
CTGGCTCTCA GTAACAGTGC TAACCCTGTG TTGTCCATAG ACAGCTACCT CAACCTTGGA 
CCAGAGGTTC AGGTGTGTTA TGTGAGCTCG CGACCTCATT CGGTGAATGT AGATCATCAA 
GGTGTCATTT TCAGTGGCCT GCTGCTCTAC TTGTGTGACT CCTTTGTCGT TTCAAGCCTT 
CTAAAGAAAT TCAACTTCCT CAAAGGCGCC ACGCTGTGTG TGATCTGTCA GGACCGGAGC 
TCTCTGCGTC AGACTATTGT ACGTCTGGAG CTGGAGGATG AGTGGCAATT TCGCCTGCGA 
GATGAGTTTC AAACGGCCAA CTGCAGCGAG GACCGTCCGC TCTACTTCCT CACAGGACGA 
CATATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001344452.4
CDS	348..6176
Translation	MGNSYAGQLKSARFEEALHNSIEASLRSSGGDPQPVFTQLYLEPDQYSGHVEDIKPKMDLSLRSDPSTHVLVKCHSSNSVEDMDDEDDSDTSSPPLPYLQGPPPDGCCTVDGFCQAGKDLRLVSMATESIEVPAGFELVGAKSPSIPEHILVCAVDKRFLPDENGKNALLGFSGNCVGCGEKGFRYFTEFSNHINLKLSTQPKKQKHLKYYLVKNSQGALCKGALICWKDCKTRPFSNSASSSKPSSSSSLSSKENGDTNGHSPSPFPLSDSPPARMQSGSSSGIFGPQELGFLKPLNTPTHGTKTLPIVPTALRVNGLTNGLSMDGRSTLLSPPRTNPLSTPSHGYRTTETGDSPASTAMSTGPPKKRHRSWHPTTLVPIPATAVPVPAIRPLTCSSGPLLSLSNQQPASVSGVIQPQPITAGETVIIPDNLLNSYGVRPVLLIGQGTLPYFFGNVGDLVVSPLLVSCYKGRELNEKTLASLGMSANQLLTTETMILLTLQYLARLGTEQIPLREEFEQIMLKAMLCGPTGPPVSPAQLPWLARMEASVSGGSVQVLVTHGSLGEGISESLRSLSETSPQQQQCLPNYVLIICTSKSGANEFCVLVLGKYQSRALAESMLSTNEFLKEISYELITGKVSVLASHFQSTSLGDNMDKQLVRYQRKRKDRVVQPFQGHLTEYIHSQEAATMIPESGPDLLSDDFQIHPPQLSVARSLLSQVCAIADSGSQSLDLGRFCKVDFLILVPPSHVLVHQTVQRIRQSGVLIDLGIEDVSLAMQKSDKYVVRLDTEVHTKMEAFMRKVKQNPYTLFVLIHDNSHVDLTSALSGSVCHGELQGLADRVVNCPEVLEAINLLVLQVSCFPFTLQSRQSRISTQNEVHWPDTENQQGEASPKDLIYFGLKDYSKSLQWGVASPILRCDDAFERMVKTLLERHPHLHSMVIRSYLLIQQYTEALMALTAAPSLRDHVTPQTLAMVEDLLSVPGRSKHGCGHMLLVRVPSLQLARLAQERLEEARDKLGLQYRFAVLLGSPAAEISLPVHFCARLRAWRGCKNEEWVPHTYEDLEGLPCIVILTGKDPLGETFPRSLKYCDLRLIDSSYLTRTALEQEVGLACSYVTRRVIPKTKTATSREERPREGERSSGETAEHDDLPMELERPPSNASAATRTSGSTTENGVSSSSILDKPSSQSDPCGSRTMMDSCSSPVRFKQECDSQAPSSSSTSSFSSASSSSSSSSSPAAQRPSQSTQAPRECNRTQVFPRTAVLSRAAYTLLAPETLGHPSSASLLPHADVSWSSPLRPPVPHGLGGAEQSLYYRQWTTARQHHADYEGPVPHPHPRRLLLSGPPQVGKTGAYLQFLRILFRMLIRLLEVDVYDEEELEEDVQDKSKVPPSSGPQWPDVEDVRKLRFDLCPHDCKFKYSSPVYANRMPKTQSGVKTERLDTEADPPKRNTVSVRLSLFAAHNAFHHCEQCHHYSEPIPAAQLSDCTFHAFTFCSSMLGEEVQLHFIIPKSKESHFVFSQQGSHLESMRLPLLSDKESGMMKSPIFTPTTGRQEHGLLNIYHAMEGAEHLHILVVKQYEMPLYRKYWPNHILLVLPAMFNNSGVGAARFMIKELSYHNLELERNRQEEQGVKRQDVWPFIVMMDDSCVLWNAQQPGPDGKTEVMNVSLKSVLQHMEATPKISQYAVCGLRKWSSSLSSQAPTSPFSRCHLHDLILLNVDLTQNVQYDLNRFTCEEVDFNLRANSSGLLLCRFNQFSIMKKHIPIGGHKDFLIKPKLMRIETPVRVCASQYVCAPDSEQTLLAAPAQFLLEKFLQSCSHRLFPLALSNSANPVLSIDSYLNLGPEVQVCYVSSRPHSVNVDHQGVIFSGLLLYLCDSFVVSSLLKKFNFLKGATLCVICQDRSSLRQTIVRLELEDEWQFRLRDEFQTANCSEDRPLYFLTGRHI

Target ORF information:

RefSeq Version	XM_001344416.7
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio growth regulation by estrogen in breast cancer-like (greb1l), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001344416.7

ORF Insert Sequence:

ATGGGAAATT CATACGCGGG GCAGCTGAAA TCTGCTCGCT TTGAGGAAGC TCTCCATAAC 
TCAATAGAGG CTTCATTAAG GTCAAGCGGT GGTGACCCAC AGCCGGTTTT CACACAGCTA 
TATCTCGAGC CTGATCAGTA TTCTGGCCAT GTAGAAGATA TTAAGCCCAA AATGGACCTA 
AGCCTGAGAT CCGACCCATC CACCCATGTG CTTGTGAAAT GCCACTCTTC AAATTCTGTA 
GAGGATATGG ATGACGAGGA CGATTCAGAC ACCAGCAGCC CACCATTGCC TTACCTGCAG 
GGACCCCCTC CTGATGGCTG CTGCACTGTT GATGGTTTCT GTCAAGCAGG AAAAGATCTG 
CGCCTGGTTT CCATGGCAAC AGAGTCTATC GAGGTTCCTG CAGGGTTTGA GCTGGTCGGT 
GCCAAATCGC CCAGCATCCC AGAGCACATC CTCGTCTGTG CAGTGGACAA GCGCTTCCTG 
CCAGATGAGA ATGGGAAAAA TGCACTTTTA GGGTTTTCAG GGAACTGCGT GGGTTGTGGT 
GAGAAAGGGT TCCGTTACTT CACCGAGTTC TCTAACCACA TTAACCTGAA GTTATCCACT 
CAGCCGAAGA AGCAGAAGCA CTTAAAGTAC TACCTGGTGA AGAACTCTCA GGGTGCTCTG 
TGCAAAGGAG CCCTCATTTG CTGGAAAGAT TGTAAGACAC GACCATTCTC AAACAGTGCT 
TCTTCATCCA AGCCTAGTTC CTCATCCTCA CTAAGCAGCA AAGAAAATGG AGACACAAAT 
GGCCACAGTC CATCTCCCTT CCCCCTCTCA GACTCGCCTC CTGCCCGCAT GCAATCTGGC 
TCATCCTCAG GGATTTTTGG ACCCCAGGAG CTGGGCTTCC TTAAACCATT GAACACACCG 
ACCCACGGGA CCAAGACATT ACCGATCGTT CCCACTGCCC TCCGAGTGAA TGGTCTAACT 
AATGGTCTCA GTATGGATGG ACGCTCCACT CTACTGAGCC CACCTCGTAC AAACCCACTC 
TCCACCCCTA GTCATGGCTA TAGAACCACA GAAACAGGAG ACAGTCCAGC ATCCACTGCT 
ATGTCGACAG GTCCACCAAA AAAGCGGCAT CGTAGTTGGC ATCCCACCAC TTTAGTGCCC 
ATCCCAGCCA CAGCTGTTCC TGTACCAGCC ATCCGACCGC TGACCTGCAG CTCTGGTCCT 
TTATTGTCAC TGTCCAATCA ACAGCCTGCA TCGGTGTCAG GGGTCATACA ACCACAGCCA 
ATCACAGCTG GAGAAACGGT CATCATTCCT GATAACCTTC TGAACTCTTA CGGGGTCCGG 
CCAGTCCTTC TCATTGGTCA AGGCACTTTA CCATATTTCT TTGGCAATGT GGGTGATCTG 
GTGGTGAGCC CCCTGCTGGT GAGCTGCTAC AAGGGCAGAG AGCTCAACGA GAAGACCCTG 
GCCAGCCTGG GCATGTCAGC AAACCAGTTA CTGACCACAG AGACCATGAT ACTGCTCACA 
CTGCAGTACC TCGCTCGACT GGGTACAGAG CAGATACCGT TACGGGAAGA GTTTGAGCAA 
ATCATGTTGA AGGCTATGCT GTGTGGTCCG ACTGGCCCTC CAGTGTCGCC TGCGCAGCTG 
CCCTGGTTAG CCCGAATGGA GGCCAGTGTG TCAGGGGGCA GTGTTCAGGT GCTGGTCACC 
CACGGCTCTC TTGGTGAGGG TATCTCTGAG TCTCTGCGCT CACTGAGTGA AACATCACCA 
CAGCAGCAGC AGTGTCTCCC CAACTATGTG CTCATTATCT GCACCTCCAA AAGTGGAGCC 
AATGAGTTCT GTGTGCTCGT GTTGGGTAAG TATCAGTCTC GAGCCTTGGC AGAGAGCATG 
CTGTCTACAA ACGAGTTTCT GAAGGAGATC AGTTATGAGC TCATCACAGG GAAAGTTAGT 
GTACTAGCCT CACACTTCCA GAGCACCTCT TTAGGTGACA ATATGGACAA ACAGCTGGTT 
CGTTATCAAC GTAAACGCAA AGACAGGGTG GTTCAACCTT TTCAGGGGCA TCTCACCGAG 
TACATCCACT CCCAGGAAGC TGCGACCATG ATACCAGAAT CAGGGCCAGA TCTGCTGAGC 
GATGACTTTC AGATACACCC TCCTCAGTTG AGCGTTGCAC GAAGCCTTTT GTCACAGGTT 
TGTGCTATTG CCGACTCAGG GAGCCAGAGC TTAGATCTGG GCCGATTCTG CAAAGTAGAC 
TTTCTGATTC TGGTGCCGCC CTCCCATGTT CTGGTTCACC AAACAGTCCA GCGAATACGA 
CAATCAGGCG TCCTAATAGA TCTCGGTATA GAGGACGTGT CATTAGCCAT GCAGAAGTCT 
GACAAGTATG TGGTGCGTCT GGACACAGAG GTCCACACAA AAATGGAGGC CTTTATGAGG 
AAGGTCAAGC AGAACCCTTA TACACTCTTC GTCCTCATTC ATGACAACTC GCACGTGGAC 
TTGACAAGTG CTCTTTCAGG GTCGGTTTGT CATGGGGAGC TGCAGGGATT GGCTGATCGC 
GTGGTGAACT GTCCAGAGGT TCTGGAAGCA ATAAACTTAC TGGTGCTGCA GGTCAGCTGC 
TTCCCGTTCA CCCTGCAGAG CCGGCAGTCA CGAATCAGTA CCCAGAATGA AGTGCACTGG 
CCCGACACTG AGAACCAGCA GGGTGAAGCT TCTCCTAAAG ACTTGATCTA CTTTGGGCTG 
AAGGACTACA GCAAGTCTCT GCAGTGGGGA GTGGCGAGTC CAATTCTGCG ATGTGATGAC 
GCCTTTGAGC GGATGGTTAA AACCCTGTTG GAGAGGCACC CACATTTGCA CAGTATGGTG 
ATCCGCAGCT ACCTGTTGAT CCAGCAGTAC ACAGAGGCTC TGATGGCCCT CACAGCGGCC 
CCTTCTTTGC GGGATCACGT GACCCCTCAG ACACTTGCCA TGGTGGAGGA TCTGCTGAGT 
GTGCCAGGCC GCAGCAAGCA TGGGTGCGGA CACATGCTGC TGGTGCGTGT GCCTTCTCTG 
CAGCTGGCAC GACTGGCACA GGAGCGGCTG GAGGAGGCTC GGGACAAGCT GGGGCTGCAG 
TACCGCTTTG CCGTGCTGCT AGGAAGCCCT GCTGCGGAGA TCAGCCTGCC GGTGCACTTC 
TGTGCCCGTC TGCGGGCTTG GAGGGGCTGT AAAAATGAGG AGTGGGTCCC ACATACATAT 
GAAGATCTGG AAGGACTTCC ATGCATTGTC ATCCTCACTG GAAAAGATCC TCTTGGAGAA 
ACCTTCCCGA GGTCTCTAAA GTACTGTGAC TTGCGGCTGA TTGACTCCAG CTACCTGACA 
CGCACAGCGC TGGAACAGGA AGTGGGTTTG GCCTGTTCAT ATGTGACACG TAGAGTAATC 
CCTAAGACCA AAACTGCCAC AAGCAGAGAA GAGAGACCGA GAGAAGGTGA AAGGAGCTCT 
GGAGAAACAG CAGAACACGA TGATCTCCCT ATGGAGCTGG AAAGACCACC CAGCAATGCC 
AGCGCTGCTA CTAGGACCTC CGGCTCCACC ACAGAAAATG GTGTCAGTTC CTCAAGTATA 
CTTGACAAGC CATCCTCGCA GAGTGACCCT TGTGGAAGCA GGACTATGAT GGACTCTTGC 
TCCTCTCCTG TTCGCTTCAA GCAGGAGTGC GACTCTCAGG CTCCCTCATC ATCCAGCACG 
TCCTCATTCT CCTCTGCTTC CTCCTCCTCT TCTTCTTCCT CGTCTCCGGC AGCTCAGAGA 
CCGAGTCAGT CTACACAGGC TCCACGTGAA TGTAACAGGA CACAGGTCTT TCCCCGGACG 
GCGGTGCTGT CTCGGGCTGC GTATACCCTG CTGGCTCCTG AAACTCTGGG TCATCCCAGT 
TCTGCCTCTC TGCTGCCACA TGCGGATGTG TCGTGGAGCA GTCCGCTCAG ACCACCGGTT 
CCTCATGGCC TTGGTGGAGC AGAGCAGTCC CTCTACTATC GTCAGTGGAC CACAGCCAGG 
CAACACCACG CTGATTATGA AGGCCCAGTA CCCCATCCCC ACCCAAGAAG ACTGCTTCTG 
AGCGGCCCAC CACAGGTTGG AAAAACAGGT GCATATCTGC AGTTCCTACG AATTCTGTTC 
CGTATGCTGA TTCGCCTTCT GGAGGTAGAT GTTTATGATG AAGAAGAGCT GGAGGAAGAT 
GTGCAAGACA AATCAAAGGT TCCCCCTTCT AGTGGGCCAC AGTGGCCTGA TGTGGAGGAC 
GTCCGCAAAC TAAGATTTGA CCTCTGCCCT CATGACTGCA AGTTCAAATA TTCAAGTCCA 
GTGTATGCCA ACAGGATGCC AAAAACACAG AGTGGGGTTA AAACCGAAAG ACTGGATACA 
GAAGCGGACC CACCAAAGAG AAACACAGTA TCTGTTAGAC TCAGCCTTTT TGCAGCCCAC 
AATGCATTTC ACCACTGTGA GCAGTGCCAC CATTACAGTG AACCTATTCC TGCTGCTCAG 
CTCTCTGACT GCACCTTCCA CGCTTTCACC TTTTGCTCAT CTATGCTGGG AGAGGAAGTC 
CAGCTGCACT TTATCATCCC TAAATCCAAA GAGTCTCATT TTGTGTTCAG CCAACAGGGC 
AGTCACCTAG AGAGCATGAG ACTGCCTCTG CTTTCCGACA AGGAGTCGGG CATGATGAAA 
AGCCCAATAT TCACCCCAAC CACGGGCCGC CAGGAGCACG GCCTTCTCAA CATCTACCAT 
GCCATGGAGG GAGCCGAGCA CCTGCACATC CTGGTGGTCA AACAGTATGA AATGCCACTC 
TATAGGAAAT ACTGGCCCAA CCACATCCTG CTGGTGCTGC CCGCCATGTT CAACAACTCA 
GGAGTCGGTG CGGCTCGATT CATGATCAAA GAACTGTCCT ATCACAACCT GGAGCTTGAG 
AGGAACCGAC AAGAGGAGCA AGGCGTCAAA CGGCAGGACG TGTGGCCCTT TATCGTAATG 
ATGGATGACT CCTGCGTCCT GTGGAACGCC CAGCAGCCTG GACCAGACGG CAAAACCGAG 
GTGATGAACG TGTCTCTGAA GAGCGTCTTG CAGCACATGG AAGCCACTCC AAAGATCTCC 
CAGTATGCAG TTTGTGGCCT GCGCAAATGG AGCAGTAGTT TGTCTTCTCA AGCTCCCACA 
AGCCCTTTCT CTCGCTGTCA TCTCCACGAC CTCATCTTGC TGAATGTTGA CCTCACACAG 
AACGTCCAGT ATGACCTCAA TCGGTTCACA TGTGAAGAGG TGGACTTCAA CCTGAGGGCA 
AACAGCAGTG GTCTTCTGCT CTGTCGCTTC AATCAATTCA GCATCATGAA GAAACACATT 
CCCATCGGAG GACATAAAGA CTTCCTCATC AAACCCAAGC TCATGAGAAT AGAGACGCCG 
GTGCGTGTGT GTGCGTCTCA ATATGTGTGC GCTCCGGACA GTGAACAAAC GTTGCTGGCT 
GCGCCAGCTC AGTTCCTGCT GGAGAAGTTT CTGCAGTCCT GTAGCCATCG GCTCTTTCCT 
CTGGCTCTCA GTAACAGTGC TAACCCTGTG TTGTCCATAG ACAGCTACCT CAACCTTGGA 
CCAGAGGTTC AGGTGTGTTA TGTGAGCTCG CGACCTCATT CGGTGAATGT AGATCATCAA 
GGTGTCATTT TCAGTGGCCT GCTGCTCTAC TTGTGTGACT CCTTTGTCGT TTCAAGCCTT 
CTAAAGAAAT TCAACTTCCT CAAAGGCGCC ACGCTGTGTG TGATCTGTCA GGACCGGAGC 
TCTCTGCGTC AGACTATTGT ACGTCTGGAG CTGGAGGATG AGTGGCAATT TCGCCTGCGA 
GATGAGTTTC AAACGGCCAA CTGCAGCGAG GACCGTCCGC TCTACTTCCT CACAGGACGA 
CATATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Exome-wide Association Study Identifies GREB1L Mutations in Congenital Kidney Malformations.
American journal of human genetics101(5)789-802(2017 Nov)
Sanna-Cherchi S,Khan K,Westland R,Krithivasan P,Fievet L,Rasouly HM,Ionita-Laza I,Capone VP,Fasel DA,Kiryluk K,Kamalakaran S,Bodria M,Otto EA,Sampson MG,Gillies CE,Vega-Warner V,Vukojevic K,Pediaditakis I,Makar GS,Mitrotti A,Verbitsky M,Martino J,Liu Q,Na YJ,Goj V,Ardissino G,Gigante M,Gesualdo L,Janezcko M,Zaniew M,Mendelsohn CL,Shril S,Hildebrandt F,van Wijk JAE,Arapovic A,Saraga M,Allegri L,Izzi C,Scolari F,Tasic V,Ghiggeri GM,Latos-Bielenska A,Materna-Kiryluk A,Mane S,Goldstein DB,Lifton RP,Katsanis N,Davis EE,Gharavi AG

A Gene Implicated in Activation of Retinoic Acid Receptor Targets Is a Novel Renal Agenesis Gene in Humans.
Genetics207(1)215-228(2017 09)
Brophy PD,Rasmussen M,Parida M,Bonde G,Darbro BW,Hong X,Clarke JC,Peterson KA,Denegre J,Schneider M,Sussman CR,Sunde L,Lildballe DL,Hertz JM,Cornell RA,Murray SA,Manak JR

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

mafba is a downstream transcriptional effector of Vegfc signaling essential for embryonic lymphangiogenesis in zebrafish.
Genes & development29(15)1618-30(2015 Aug)
Koltowska K,Paterson S,Bower NI,Baillie GJ,Lagendijk AK,Astin JW,Chen H,Francois M,Crosier PS,Taft RJ,Simons C,Smith KA,Hogan BM

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GREB1L cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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