dyrk1aa cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	dyrk1aa
Entrez Gene ID	100005019
Full Name	dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1A, a
Synonyms	dyrk1a,im:6962097,zgc:158359
General protein information	Preferred Names dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1A, a Names dual specificity tyrosine-phosphorylation-regulated kinase 1A
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009305034.2	XP_009303309.1	dual specificity tyrosine-phosphorylation-regulated kinase 1A isoform X1
NM_001080689.1	NP_001074158.1	dual specificity tyrosine-phosphorylation-regulated kinase 1A isoform 1
NM_001347739.1	NP_001334668.1	dual specificity tyrosine-phosphorylation-regulated kinase 1A isoform 2
XM_009305034.3	XP_009303309.2	dual specificity tyrosine-phosphorylation-regulated kinase 1A isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	DYRK1A	NP_001387.2
	DYRK1A	XP_001083622.1
Gallus gallus (chicken)	DYRK1A	NP_989881.1
Rattus norvegicus (Norway rat)	Dyrk1a	NP_036923.1
Bos taurus (cattle)	DYRK1A	NP_001192943.1
Canis lupus familiaris (dog)	DYRK1A	XP_849580.2
Mus musculus (house mouse)	Dyrk1a	NP_001106860.1
Pan troglodytes (chimpanzee)	DYRK1A	XP_514894.2
Danio rerio (zebrafish)	dyrk1aa	NP_001074158.1
Xenopus tropicalis (tropical clawed frog)	dyrk1a	NP_001072280.1

Gene Ontology
Bibliography

Related articles in PubMed

Overexpression of DYRK1A, a Down Syndrome Candidate gene, Impairs Primordial Germ Cells Maintenance and Migration in zebrafish.
Liu Y, Lin Z, Liu M, Wang H, Sun H
Scientific reports7(1)15313(2017 Nov)

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Zebrafish knockout of Down syndrome gene,
Kim OH, Cho HJ, Han E, Hong TI, Ariyasiri K, Choi JH, Hwang KS, Jeong YM, Yang SY, Yu K, Park DS, Oh HW, Davis EE, Schwartz CE, Lee JS, Kim HG, Kim CH
Molecular autism850(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following dyrk1aa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dyrk1aa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa05053	NM_001080689.1 Latest version!	Danio rerio dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1A, a (dyrk1aa), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa47984	XM_009305034.2 Latest version!	Danio rerio dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1A, a (dyrk1aa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa98401	NM_001347739.1 Latest version!	Danio rerio dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1A, a (dyrk1aa), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa98401	XM_009305034.3 Latest version!	Danio rerio dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1A, a (dyrk1aa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dyrk1aa ( NM_001080689.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001074158.1 Danio rerio dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1A, a (dyrk1aa), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ODa05053
Clone ID Related Accession (Same CDS sequence)	NM_001080689.1
Accession Version	NM_001080689.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2214bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Danio rerio(zebrafish)
Product	dual specificity tyrosine-phosphorylation-regulated kinase 1A isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC129212.1. On Mar 25, 2008 this sequence version replaced XM_001344140.1. ##Evidence-Data-START## Transcript exon combination :: BC129212.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTGCTGCA TTGTGTTTGC TGGAGGAGAG ACCTCAGCAT GCAAACCCTC CTCTGTCCGG 
CTGGCCCCCT CGTTTTCTTT CCACTCGGCT GGTGTGCAGA TGGATGCGCA GACGCCCCAT 
TCTCACCCGC CGTTCAGTGA TGCTCACCCA CAGGGCGCCG CTGACCAATC CGCTGCTGCG 
CTGCCCTACA ACCCGCAGGC CCCGCAGCCC AGCGCCACCC AGGTAACTGC TGATATGGTG 
ATGCTGCAGA GGCGGATGCC TCAGTGTTTC CGGGATCCAG CGACGGCACC CTTAAGAAAA 
CTCTCCATCG AGCTGATCAA AACATACAAA CACATCAATG AGGTTTACTA TGCAAAAAAG 
AAGCGTCGTC ACCAACAGGG TCAGGGCGAG GACTCCAGCA ACAAGAAGGA GAGGAAGATT 
TACAACGACG GCTATGACGA CGACAACTTC GACTACATCG TTAAAAATGG CGAGAAGTGG 
ATGGACCGAT ATGAGATCGA CTCTTTAATA GGGAAAGGCT CGTTCGGGCA GGTGGTGAAG 
GCGTACGACC GTGTGGAGCA GGAGTGGGTC GCCATCAAGA TCATAAAGAA TAAAAAGGCC 
TTCCTGAATC AGGCTCAGAT CGAAGTGCGA CTGCTGGAGC TGATGAACAA GCACGACACC 
GAGATGAAGT ACTACATCGT TCACCTGAAA CGCCACTTCA TGTTCCGAAA CCACCTGTGT 
CTGGTGTTCG AGATGCTGTC CTATAACCTG TACGATCTGC TGAGGAACAC CAACTTCCGC 
GGCGTGTCCC TGAACCTGAC GCGTAAGTTC GCCCAGCAGC TGTGCACCGC GCTGCTGTTC 
CTGGCCACGC CGGAGCTCAG CATCATCCAC TGTGACCTCA AGCCTGAAAA CATCCTGCTC 
TGCAACCCCA AGAGGTCCGC CATCAAGATC GTGGACTTCG GGAGCTCCTG CCAGCTGGGC 
CAGCGGATAT ACCAGTACAT CCAGAGTCGC TTCTACCGCT CGCCCGAGGT CCTGCTGGGG 
ATGCCGTACG ATCTGGCCAT CGACATGTGG TCTCTAGGCT GCATCCTGGT GGAGATGCAC 
ACCGGAGAAC CGCTCTTCAG CGGCGCTAAT GAGGTTGATC AGATGAATAA GATCGTGGAG 
GTGTTGGGCG TTCCTCCCAG TCATATCCTG GAGCAGGCAC CTAAAGCCAG GAAGTTCTTT 
GAGAAGATGC CGGACAGTAT GTGGTGCGCC AAGAAGACCA AAGACGGCAA AAGGTATAAG 
CCTGCTGGCT CCCGGAAGCT GCACAGTATT CTGGGTGTGG AGGCAGGCGG CCCCGGCGGG 
CGCAGAGCCG GAGAGTCGGG TCACGCCGTC GCAGACTACC TGAAGTTCAA GGACCTGATC 
CTGCGGATGC TAGACTATGA CCCCAAGACG CGCATCCAGC CGTACTACGC TCTGCAGCAC 
AGCTTCTTCA AGAAGACAGC AGACGAGGGC ACCAACACCA GCAGCAGTGT GTCCACCAGC 
CCCGCGCTGG AGCAGTCGCA GTCCTCCGGG ACCACCTCCA GCACCTCCTC CAGCTCAGGA 
GGCTCGTCTG GAACCAGCAC CAGTGGCCGC GCCCGCTCAG ACCCCACACA TCAGCACCGG 
CACAGCGGAG GAGGACACTT CAGTGCTGCG GTGGCCATGG ACTGCCACAA CCTCTGCCCA 
CAGGCAAGAC AGGCGTTTGT GCCGGCGCTG GGCTGGGTTG GAGACGGACT CCAGCAGGCG 
TCAGGAGAGA CTCACCCCGT ACAGGAGACC ACTTTCCACG TGCCCCCACA CCAGCCCAAA 
GCCCTGCACC CCCACCAACA CCACCACCAT CATCACCACC ACCCGCCGCC GCATGGACAG 
GGCCCTGCCC GACCTCGACC CCGGCTCTAC TCTCCGTCCC ACAGCGCCTC CACGCAGGAC 
TCAATGGAGG TGACGCACGG ACACCTGTCC ATGACCTCGC TGTCTTCCTC CGCCTCGTCC 
TCCTCCACCT CCTCCTCCTC CACCGGCAAC CATCACTACG CCTACCAGAG CCGCCCGCTG 
CCCCACGGCC TGGGCTTCGG CCACAGCGGC AGCATGACTC TGGGCCTGGG TGCCTTCTCC 
AACCCGCGAC AGGAGACGGG CGTGGCAGGC GTAGGTCTGC GGCCGGGTCT GGAGCGCGAG 
GAGTCCCCGA TGGCGGGTGT ATGTGTGCAG CAGAGCTCCG TGGCCAGCTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074158.1
CDS	17..2230
Translation	MCCIVFAGGETSACKPSSVRLAPSFSFHSAGVQMDAQTPHSHPPFSDAHPQGAADQSAAALPYNPQAPQPSATQVTADMVMLQRRMPQCFRDPATAPLRKLSIELIKTYKHINEVYYAKKKRRHQQGQGEDSSNKKERKIYNDGYDDDNFDYIVKNGEKWMDRYEIDSLIGKGSFGQVVKAYDRVEQEWVAIKIIKNKKAFLNQAQIEVRLLELMNKHDTEMKYYIVHLKRHFMFRNHLCLVFEMLSYNLYDLLRNTNFRGVSLNLTRKFAQQLCTALLFLATPELSIIHCDLKPENILLCNPKRSAIKIVDFGSSCQLGQRIYQYIQSRFYRSPEVLLGMPYDLAIDMWSLGCILVEMHTGEPLFSGANEVDQMNKIVEVLGVPPSHILEQAPKARKFFEKMPDSMWCAKKTKDGKRYKPAGSRKLHSILGVEAGGPGGRRAGESGHAVADYLKFKDLILRMLDYDPKTRIQPYYALQHSFFKKTADEGTNTSSSVSTSPALEQSQSSGTTSSTSSSSGGSSGTSTSGRARSDPTHQHRHSGGGHFSAAVAMDCHNLCPQARQAFVPALGWVGDGLQQASGETHPVQETTFHVPPHQPKALHPHQHHHHHHHHPPPHGQGPARPRPRLYSPSHSASTQDSMEVTHGHLSMTSLSSSASSSSTSSSSTGNHHYAYQSRPLPHGLGFGHSGSMTLGLGAFSNPRQETGVAGVGLRPGLEREESPMAGVCVQQSSVASS

Target ORF information:

RefSeq Version	NM_001080689.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1A, a (dyrk1aa), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001080689.1

ORF Insert Sequence:

ATGTGCTGCA TTGTGTTTGC TGGAGGAGAG ACCTCAGCAT GCAAACCCTC CTCTGTCCGG 
CTGGCCCCCT CGTTTTCTTT CCACTCGGCT GGTGTGCAGA TGGATGCGCA GACGCCCCAT 
TCTCACCCGC CGTTCAGTGA TGCTCACCCA CAGGGCGCCG CTGACCAATC CGCTGCTGCG 
CTGCCCTACA ACCCGCAGGC CCCGCAGCCC AGCGCCACCC AGGTAACTGC TGATATGGTG 
ATGCTGCAGA GGCGGATGCC TCAGTGTTTC CGGGATCCAG CGACGGCACC CTTAAGAAAA 
CTCTCCATCG AGCTGATCAA AACATACAAA CACATCAATG AGGTTTACTA TGCAAAAAAG 
AAGCGTCGTC ACCAACAGGG TCAGGGCGAG GACTCCAGCA ACAAGAAGGA GAGGAAGATT 
TACAACGACG GCTATGACGA CGACAACTTC GACTACATCG TTAAAAATGG CGAGAAGTGG 
ATGGACCGAT ATGAGATCGA CTCTTTAATA GGGAAAGGCT CGTTCGGGCA GGTGGTGAAG 
GCGTACGACC GTGTGGAGCA GGAGTGGGTC GCCATCAAGA TCATAAAGAA TAAAAAGGCC 
TTCCTGAATC AGGCTCAGAT CGAAGTGCGA CTGCTGGAGC TGATGAACAA GCACGACACC 
GAGATGAAGT ACTACATCGT TCACCTGAAA CGCCACTTCA TGTTCCGAAA CCACCTGTGT 
CTGGTGTTCG AGATGCTGTC CTATAACCTG TACGATCTGC TGAGGAACAC CAACTTCCGC 
GGCGTGTCCC TGAACCTGAC GCGTAAGTTC GCCCAGCAGC TGTGCACCGC GCTGCTGTTC 
CTGGCCACGC CGGAGCTCAG CATCATCCAC TGTGACCTCA AGCCTGAAAA CATCCTGCTC 
TGCAACCCCA AGAGGTCCGC CATCAAGATC GTGGACTTCG GGAGCTCCTG CCAGCTGGGC 
CAGCGGATAT ACCAGTACAT CCAGAGTCGC TTCTACCGCT CGCCCGAGGT CCTGCTGGGG 
ATGCCGTACG ATCTGGCCAT CGACATGTGG TCTCTAGGCT GCATCCTGGT GGAGATGCAC 
ACCGGAGAAC CGCTCTTCAG CGGCGCTAAT GAGGTTGATC AGATGAATAA GATCGTGGAG 
GTGTTGGGCG TTCCTCCCAG TCATATCCTG GAGCAGGCAC CTAAAGCCAG GAAGTTCTTT 
GAGAAGATGC CGGACAGTAT GTGGTGCGCC AAGAAGACCA AAGACGGCAA AAGGTATAAG 
CCTGCTGGCT CCCGGAAGCT GCACAGTATT CTGGGTGTGG AGGCAGGCGG CCCCGGCGGG 
CGCAGAGCCG GAGAGTCGGG TCACGCCGTC GCAGACTACC TGAAGTTCAA GGACCTGATC 
CTGCGGATGC TAGACTATGA CCCCAAGACG CGCATCCAGC CGTACTACGC TCTGCAGCAC 
AGCTTCTTCA AGAAGACAGC AGACGAGGGC ACCAACACCA GCAGCAGTGT GTCCACCAGC 
CCCGCGCTGG AGCAGTCGCA GTCCTCCGGG ACCACCTCCA GCACCTCCTC CAGCTCAGGA 
GGCTCGTCTG GAACCAGCAC CAGTGGCCGC GCCCGCTCAG ACCCCACACA TCAGCACCGG 
CACAGCGGAG GAGGACACTT CAGTGCTGCG GTGGCCATGG ACTGCCACAA CCTCTGCCCA 
CAGGCAAGAC AGGCGTTTGT GCCGGCGCTG GGCTGGGTTG GAGACGGACT CCAGCAGGCG 
TCAGGAGAGA CTCACCCCGT ACAGGAGACC ACTTTCCACG TGCCCCCACA CCAGCCCAAA 
GCCCTGCACC CCCACCAACA CCACCACCAT CATCACCACC ACCCGCCGCC GCATGGACAG 
GGCCCTGCCC GACCTCGACC CCGGCTCTAC TCTCCGTCCC ACAGCGCCTC CACGCAGGAC 
TCAATGGAGG TGACGCACGG ACACCTGTCC ATGACCTCGC TGTCTTCCTC CGCCTCGTCC 
TCCTCCACCT CCTCCTCCTC CACCGGCAAC CATCACTACG CCTACCAGAG CCGCCCGCTG 
CCCCACGGCC TGGGCTTCGG CCACAGCGGC AGCATGACTC TGGGCCTGGG TGCCTTCTCC 
AACCCGCGAC AGGAGACGGG CGTGGCAGGC GTAGGTCTGC GGCCGGGTCT GGAGCGCGAG 
GAGTCCCCGA TGGCGGGTGT ATGTGTGCAG CAGAGCTCCG TGGCCAGCTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dyrk1aa ( XM_009305034.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303309.1 Danio rerio dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1A, a (dyrk1aa), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa47984
Clone ID Related Accession (Same CDS sequence)	XM_009305034.2
Accession Version	XM_009305034.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	dual specificity tyrosine-phosphorylation-regulated kinase 1A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) and transcript sequence (BC129212.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305034.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 27 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGATGCGC AGACGCCCCA TTCTCACCCG CCGTTCAGTG ATGCTCACCC ACAGGGCGCC 
GCTGACCAAT CCGCTGCTGC GCTGCCCTAC AACCCGCAGG CCCCGCAGCC CAGCGCCACC 
CAGGTAACTG CTGATATGGT GATGCTGCAG AGGCGGATGC CTCAGTGTTT CCGGGATCCA 
GCGACGGCAC CCTTAAGAAA ACTCTCCATC GAGCTGATCA AAACATACAA ACACATCAAT 
GAGGTTTACT ATGCAAAAAA GAAGCGTCGT CACCAACAGG GTCAGGGCGA GGACTCCAGC 
AACAAGAAGG AGAGGAAGAT TTACAACGAC GGCTATGACG ACGACAACTT CGACTACATC 
GTTAAAAATG GCGAGAAGTG GATGGACCGA TATGAGATCG ACTCTTTAAT AGGGAAAGGC 
TCGTTCGGGC AGGTGGTGAA GGCGTACGAC CGTGTGGAGC AGGAGTGGGT CGCCATCAAG 
ATCATAAAGA ATAAAAAGGC CTTCCTGAAT CAGGCTCAGA TCGAAGTGCG ACTGCTGGAG 
CTGATGAACA AGCACGACAC CGAGATGAAG TACTACATCG TTCACCTGAA ACGCCACTTC 
ATGTTCCGAA ACCACCTGTG TCTGGTGTTC GAGATGCTGT CCTATAACCT GTACGATCTG 
CTGAGGAACA CCAACTTCCG CGGCGTGTCC CTGAACCTGA CGCGTAAGTT CGCCCAGCAG 
CTGTGCACCG CGCTGCTGTT CCTGGCCACG CCGGAGCTCA GCATCATCCA CTGTGACCTC 
AAGCCTGAAA ACATCCTGCT CTGCAACCCC AAGAGGTCCG CCATCAAGAT CGTGGACTTC 
GGGAGCTCCT GCCAGCTGGG CCAGCGGATA TACCAGTACA TCCAGAGTCG CTTCTACCGC 
TCGCCCGAGG TCCTGCTGGG GATGCCGTAC GATCTGGCCA TCGACATGTG GTCTCTAGGC 
TGCATCCTGG TGGAGATGCA CACCGGAGAA CCGCTCTTCA GCGGCGCTAA TGAGGTTGAT 
CAGATGAATA AGATCGTGGA GGTGTTGGGC GTTCCTCCCA GTCATATCCT GGAGCAGGCA 
CCTAAAGCCA GGAAGTTCTT TGAGAAGATG CCGGACAGTA TGTGGTGCGC CAAGAAGACC 
AAAGACGGCA AAAGGTATAA GCCTGCTGGC TCCCGGAAGC TGCACAGTAT TCTGGGTGTG 
GAGGCAGGCG GCCCCGGCGG GCGCAGAGCC GGAGAGTCGG GTCACGCCGT CGCAGACTAC 
CTGAAGTTCA AGGACCTGAT CCTGCGGATG CTAGACTATG ACCCCAAGAC GCGCATCCAG 
CCGTACTACG CTCTGCAGCA CAGCTTCTTC AAGAAGACAG CAGACGAGGG CACCAACACC 
AGCAGCAGTG TGTCCACCAG CCCCGCGCTG GAGCAGTCGC AGTCCTCCGG GACCACCTCC 
AGCACCTCCT CCAGCTCAGG AGGCTCGTCT GGAACCAGCA CCAGTGGCCG CGCCCGCTCA 
GACCCCACAC ATCAGCACCG GCACAGCGGA GGAGGACACT TCAGTGCTGC GGTGGCCATG 
GACTGCCACA ACCTCTGCCC ACAGGCAAGA CAGGCGTTTG TGCCGGCGCT GGGCTGGGTT 
GGAGACGGAC TCCAGCAGGC GTCAGGAGAG ACTCACCCCG TACAGGAGAC CACTTTCCAC 
GTGCCCCCAC ACCAGCCCAA AGCCCTGCAC CCCCACCAAC ACCACCACCA TCATCACCAC 
CACCCGCCGC CGCATGGACA GGGCCCTGCC CGACCTCGAC CCCGGCTCTA CTCTCCGTCC 
CACAGCGCCT CCACGCAGGA CTCAATGGAG GTGACGCACG GACACCTGTC CATGACCTCG 
CTGTCTTCCT CCGCCTCGTC CTCCTCCACC TCCTCCTCCT CCACCGGCAA CCATCACTAC 
GCCTACCAGA GCCGCCCGCT GCCCCACGGC CTGGGCTTCG GCCACAGCGG CAGCATGACT 
CTGGGCCTGG GTGCCTTCTC CAACCCGCGA CAGGAGACGG GCGTGGCAGG CGTAGGTCTG 
CGGCCGGGTC TGGAGCGCGA GGAGTCCCCG ATGGCGGGTG TATGTGTGCA GCAGAGCTCC 
GTGGCCAGCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303309.1
CDS	79..2193
Translation	MDAQTPHSHPPFSDAHPQGAADQSAAALPYNPQAPQPSATQVTADMVMLQRRMPQCFRDPATAPLRKLSIELIKTYKHINEVYYAKKKRRHQQGQGEDSSNKKERKIYNDGYDDDNFDYIVKNGEKWMDRYEIDSLIGKGSFGQVVKAYDRVEQEWVAIKIIKNKKAFLNQAQIEVRLLELMNKHDTEMKYYIVHLKRHFMFRNHLCLVFEMLSYNLYDLLRNTNFRGVSLNLTRKFAQQLCTALLFLATPELSIIHCDLKPENILLCNPKRSAIKIVDFGSSCQLGQRIYQYIQSRFYRSPEVLLGMPYDLAIDMWSLGCILVEMHTGEPLFSGANEVDQMNKIVEVLGVPPSHILEQAPKARKFFEKMPDSMWCAKKTKDGKRYKPAGSRKLHSILGVEAGGPGGRRAGESGHAVADYLKFKDLILRMLDYDPKTRIQPYYALQHSFFKKTADEGTNTSSSVSTSPALEQSQSSGTTSSTSSSSGGSSGTSTSGRARSDPTHQHRHSGGGHFSAAVAMDCHNLCPQARQAFVPALGWVGDGLQQASGETHPVQETTFHVPPHQPKALHPHQHHHHHHHHPPPHGQGPARPRPRLYSPSHSASTQDSMEVTHGHLSMTSLSSSASSSSTSSSSTGNHHYAYQSRPLPHGLGFGHSGSMTLGLGAFSNPRQETGVAGVGLRPGLEREESPMAGVCVQQSSVASS

Target ORF information:

RefSeq Version	XM_009305034.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1A, a (dyrk1aa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305034.2

ORF Insert Sequence:

ATGGATGCGC AGACGCCCCA TTCTCACCCG CCGTTCAGTG ATGCTCACCC ACAGGGCGCC 
GCTGACCAAT CCGCTGCTGC GCTGCCCTAC AACCCGCAGG CCCCGCAGCC CAGCGCCACC 
CAGGTAACTG CTGATATGGT GATGCTGCAG AGGCGGATGC CTCAGTGTTT CCGGGATCCA 
GCGACGGCAC CCTTAAGAAA ACTCTCCATC GAGCTGATCA AAACATACAA ACACATCAAT 
GAGGTTTACT ATGCAAAAAA GAAGCGTCGT CACCAACAGG GTCAGGGCGA GGACTCCAGC 
AACAAGAAGG AGAGGAAGAT TTACAACGAC GGCTATGACG ACGACAACTT CGACTACATC 
GTTAAAAATG GCGAGAAGTG GATGGACCGA TATGAGATCG ACTCTTTAAT AGGGAAAGGC 
TCGTTCGGGC AGGTGGTGAA GGCGTACGAC CGTGTGGAGC AGGAGTGGGT CGCCATCAAG 
ATCATAAAGA ATAAAAAGGC CTTCCTGAAT CAGGCTCAGA TCGAAGTGCG ACTGCTGGAG 
CTGATGAACA AGCACGACAC CGAGATGAAG TACTACATCG TTCACCTGAA ACGCCACTTC 
ATGTTCCGAA ACCACCTGTG TCTGGTGTTC GAGATGCTGT CCTATAACCT GTACGATCTG 
CTGAGGAACA CCAACTTCCG CGGCGTGTCC CTGAACCTGA CGCGTAAGTT CGCCCAGCAG 
CTGTGCACCG CGCTGCTGTT CCTGGCCACG CCGGAGCTCA GCATCATCCA CTGTGACCTC 
AAGCCTGAAA ACATCCTGCT CTGCAACCCC AAGAGGTCCG CCATCAAGAT CGTGGACTTC 
GGGAGCTCCT GCCAGCTGGG CCAGCGGATA TACCAGTACA TCCAGAGTCG CTTCTACCGC 
TCGCCCGAGG TCCTGCTGGG GATGCCGTAC GATCTGGCCA TCGACATGTG GTCTCTAGGC 
TGCATCCTGG TGGAGATGCA CACCGGAGAA CCGCTCTTCA GCGGCGCTAA TGAGGTTGAT 
CAGATGAATA AGATCGTGGA GGTGTTGGGC GTTCCTCCCA GTCATATCCT GGAGCAGGCA 
CCTAAAGCCA GGAAGTTCTT TGAGAAGATG CCGGACAGTA TGTGGTGCGC CAAGAAGACC 
AAAGACGGCA AAAGGTATAA GCCTGCTGGC TCCCGGAAGC TGCACAGTAT TCTGGGTGTG 
GAGGCAGGCG GCCCCGGCGG GCGCAGAGCC GGAGAGTCGG GTCACGCCGT CGCAGACTAC 
CTGAAGTTCA AGGACCTGAT CCTGCGGATG CTAGACTATG ACCCCAAGAC GCGCATCCAG 
CCGTACTACG CTCTGCAGCA CAGCTTCTTC AAGAAGACAG CAGACGAGGG CACCAACACC 
AGCAGCAGTG TGTCCACCAG CCCCGCGCTG GAGCAGTCGC AGTCCTCCGG GACCACCTCC 
AGCACCTCCT CCAGCTCAGG AGGCTCGTCT GGAACCAGCA CCAGTGGCCG CGCCCGCTCA 
GACCCCACAC ATCAGCACCG GCACAGCGGA GGAGGACACT TCAGTGCTGC GGTGGCCATG 
GACTGCCACA ACCTCTGCCC ACAGGCAAGA CAGGCGTTTG TGCCGGCGCT GGGCTGGGTT 
GGAGACGGAC TCCAGCAGGC GTCAGGAGAG ACTCACCCCG TACAGGAGAC CACTTTCCAC 
GTGCCCCCAC ACCAGCCCAA AGCCCTGCAC CCCCACCAAC ACCACCACCA TCATCACCAC 
CACCCGCCGC CGCATGGACA GGGCCCTGCC CGACCTCGAC CCCGGCTCTA CTCTCCGTCC 
CACAGCGCCT CCACGCAGGA CTCAATGGAG GTGACGCACG GACACCTGTC CATGACCTCG 
CTGTCTTCCT CCGCCTCGTC CTCCTCCACC TCCTCCTCCT CCACCGGCAA CCATCACTAC 
GCCTACCAGA GCCGCCCGCT GCCCCACGGC CTGGGCTTCG GCCACAGCGG CAGCATGACT 
CTGGGCCTGG GTGCCTTCTC CAACCCGCGA CAGGAGACGG GCGTGGCAGG CGTAGGTCTG 
CGGCCGGGTC TGGAGCGCGA GGAGTCCCCG ATGGCGGGTG TATGTGTGCA GCAGAGCTCC 
GTGGCCAGCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dyrk1aa ( NM_001347739.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001334668.1 Danio rerio dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1A, a (dyrk1aa), transcript variant 2, mRNA.

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CloneID	ODa98401
Clone ID Related Accession (Same CDS sequence)	NM_001347739.1 , XM_009305034.3
Accession Version	NM_001347739.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Danio rerio(zebrafish)
Product	dual specificity tyrosine-phosphorylation-regulated kinase 1A isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU633885.4, EB980854.1, CABZ01076953.1, EH997539.1 and CABZ01076954.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## CDS exon combination :: GDQH01004433.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCATACAG GAGGAGAGAC CTCAGCATGC AAACCCTCCT CTGTCCGGCT GGCCCCCTCG 
TTTTCTTTCC ACTCGGCTGG TGTGCAGATG GATGCGCAGA CGCCCCATTC TCACCCGCCG 
TTCAGTGATG CTCACCCACA GGGCGCCGCT GACCAATCCG CTGCTGCGCT GCCCTACAAC 
CCGCAGGCCC CGCAGCCCAG CGCCACCCAG GTAACTGCTG ATATGGTGAT GCTGCAGAGG 
CGGATGCCTC AGTGTTTCCG GGATCCAGCG ACGGCACCCT TAAGAAAACT CTCCATCGAG 
CTGATCAAAA CATACAAACA CATCAATGAG GTTTACTATG CAAAAAAGAA GCGTCGTCAC 
CAACAGGGTC AGGGCGAGGA CTCCAGCAAC AAGAAGGAGA GGAAGATTTA CAACGACGGC 
TATGACGACG ACAACTTCGA CTACATCGTT AAAAATGGCG AGAAGTGGAT GGACCGATAT 
GAGATCGACT CTTTAATAGG GAAAGGCTCG TTCGGGCAGG TGGTGAAGGC GTACGACCGT 
GTGGAGCAGG AGTGGGTCGC CATCAAGATC ATAAAGAATA AAAAGGCCTT CCTGAATCAG 
GCTCAGATCG AAGTGCGACT GCTGGAGCTG ATGAACAAGC ACGACACCGA GATGAAGTAC 
TACATCGTTC ACCTGAAACG CCACTTCATG TTCCGAAACC ACCTGTGTCT GGTGTTCGAG 
ATGCTGTCCT ATAACCTGTA CGATCTGCTG AGGAACACCA ACTTCCGCGG CGTGTCCCTG 
AACCTGACGC GTAAGTTCGC CCAGCAGCTG TGCACCGCGC TGCTGTTCCT GGCCACGCCG 
GAGCTCAGCA TCATCCACTG TGACCTCAAG CCTGAAAACA TCCTGCTCTG CAACCCCAAG 
AGGTCCGCCA TCAAGATCGT GGACTTCGGG AGCTCCTGCC AGCTGGGCCA GCGGATATAC 
CAGTACATCC AGAGTCGCTT CTACCGCTCG CCCGAGGTCC TGCTGGGGAT GCCGTACGAT 
CTGGCCATCG ACATGTGGTC TCTAGGCTGC ATCCTGGTGG AGATGCACAC CGGAGAACCG 
CTCTTCAGCG GCGCTAATGA GGTTGATCAG ATGAATAAGA TCGTGGAGGT GTTGGGCGTT 
CCTCCCAGTC ATATCCTGGA GCAGGCACCT AAAGCCAGGA AGTTCTTTGA GAAGATGCCG 
GACAGTATGT GGTGCGCCAA GAAGACCAAA GACGGCAAAA GGTATAAGCC TGCTGGCTCC 
CGGAAGCTGC ACAGTATTCT GGGTGTGGAG GCAGGCGGCC CCGGCGGGCG CAGAGCCGGA 
GAGTCGGGTC ACGCCGTCGC AGACTACCTG AAGTTCAAGG ACCTGATCCT GCGGATGCTA 
GACTATGACC CCAAGACGCG CATCCAGCCG TACTACGCTC TGCAGCACAG CTTCTTCAAG 
AAGACAGCAG ACGAGGGCAC CAACACCAGC AGCAGTGTGT CCACCAGCCC CGCGCTGGAG 
CAGTCGCAGT CCTCCGGGAC CACCTCCAGC ACCTCCTCCA GCTCAGGAGG CTCGTCTGGA 
ACCAGCACCA GTGGCCGCGC CCGCTCAGAC CCCACACATC AGCACCGGCA CAGCGGAGGA 
GGACACTTCA GTGCTGCGGT GGCCATGGAC TGCCACAACC TCTGCCCACA GGCAAGACAG 
GCGTTTGTGC CGGCGCTGGG CTGGGTTGGA GACGGACTCC AGCAGGCGTC AGGAGAGACT 
CACCCCGTAC AGGAGACCAC TTTCCACGTG CCCCCACACC AGCCCAAAGC CCTGCACCCC 
CACCAACACC ACCACCATCA TCACCACCAC CCGCCGCCGC ATGGACAGGG CCCTGCCCGA 
CCTCGACCCC GGCTCTACTC TCCGTCCCAC AGCGCCTCCA CGCAGGACTC AATGGAGGTG 
ACGCACGGAC ACCTGTCCAT GACCTCGCTG TCTTCCTCCG CCTCGTCCTC CTCCACCTCC 
TCCTCCTCCA CCGGCAACCA TCACTACGCC TACCAGAGCC GCCCGCTGCC CCACGGCCTG 
GGCTTCGGCC ACAGCGGCAG CATGACTCTG GGCCTGGGTG CCTTCTCCAA CCCGCGACAG 
GAGACGGGCG TGGCAGGCGT AGGTCTGCGG CCGGGTCTGG AGCGCGAGGA GTCCCCGATG 
GCGGGTGTAT GTGTGCAGCA GAGCTCCGTG GCCAGCTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001334668.1
CDS	535..2736
Translation	MHTGGETSACKPSSVRLAPSFSFHSAGVQMDAQTPHSHPPFSDAHPQGAADQSAAALPYNPQAPQPSATQVTADMVMLQRRMPQCFRDPATAPLRKLSIELIKTYKHINEVYYAKKKRRHQQGQGEDSSNKKERKIYNDGYDDDNFDYIVKNGEKWMDRYEIDSLIGKGSFGQVVKAYDRVEQEWVAIKIIKNKKAFLNQAQIEVRLLELMNKHDTEMKYYIVHLKRHFMFRNHLCLVFEMLSYNLYDLLRNTNFRGVSLNLTRKFAQQLCTALLFLATPELSIIHCDLKPENILLCNPKRSAIKIVDFGSSCQLGQRIYQYIQSRFYRSPEVLLGMPYDLAIDMWSLGCILVEMHTGEPLFSGANEVDQMNKIVEVLGVPPSHILEQAPKARKFFEKMPDSMWCAKKTKDGKRYKPAGSRKLHSILGVEAGGPGGRRAGESGHAVADYLKFKDLILRMLDYDPKTRIQPYYALQHSFFKKTADEGTNTSSSVSTSPALEQSQSSGTTSSTSSSSGGSSGTSTSGRARSDPTHQHRHSGGGHFSAAVAMDCHNLCPQARQAFVPALGWVGDGLQQASGETHPVQETTFHVPPHQPKALHPHQHHHHHHHHPPPHGQGPARPRPRLYSPSHSASTQDSMEVTHGHLSMTSLSSSASSSSTSSSSTGNHHYAYQSRPLPHGLGFGHSGSMTLGLGAFSNPRQETGVAGVGLRPGLEREESPMAGVCVQQSSVASS

Target ORF information:

RefSeq Version	NM_001347739.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1A, a (dyrk1aa), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001347739.1

ORF Insert Sequence:

ATGCATACAG GAGGAGAGAC CTCAGCATGC AAACCCTCCT CTGTCCGGCT GGCCCCCTCG 
TTTTCTTTCC ACTCGGCTGG TGTGCAGATG GATGCGCAGA CGCCCCATTC TCACCCGCCG 
TTCAGTGATG CTCACCCACA GGGCGCCGCT GACCAATCCG CTGCTGCGCT GCCCTACAAC 
CCGCAGGCCC CGCAGCCCAG CGCCACCCAG GTAACTGCTG ATATGGTGAT GCTGCAGAGG 
CGGATGCCTC AGTGTTTCCG GGATCCAGCG ACGGCACCCT TAAGAAAACT CTCCATCGAG 
CTGATCAAAA CATACAAACA CATCAATGAG GTTTACTATG CAAAAAAGAA GCGTCGTCAC 
CAACAGGGTC AGGGCGAGGA CTCCAGCAAC AAGAAGGAGA GGAAGATTTA CAACGACGGC 
TATGACGACG ACAACTTCGA CTACATCGTT AAAAATGGCG AGAAGTGGAT GGACCGATAT 
GAGATCGACT CTTTAATAGG GAAAGGCTCG TTCGGGCAGG TGGTGAAGGC GTACGACCGT 
GTGGAGCAGG AGTGGGTCGC CATCAAGATC ATAAAGAATA AAAAGGCCTT CCTGAATCAG 
GCTCAGATCG AAGTGCGACT GCTGGAGCTG ATGAACAAGC ACGACACCGA GATGAAGTAC 
TACATCGTTC ACCTGAAACG CCACTTCATG TTCCGAAACC ACCTGTGTCT GGTGTTCGAG 
ATGCTGTCCT ATAACCTGTA CGATCTGCTG AGGAACACCA ACTTCCGCGG CGTGTCCCTG 
AACCTGACGC GTAAGTTCGC CCAGCAGCTG TGCACCGCGC TGCTGTTCCT GGCCACGCCG 
GAGCTCAGCA TCATCCACTG TGACCTCAAG CCTGAAAACA TCCTGCTCTG CAACCCCAAG 
AGGTCCGCCA TCAAGATCGT GGACTTCGGG AGCTCCTGCC AGCTGGGCCA GCGGATATAC 
CAGTACATCC AGAGTCGCTT CTACCGCTCG CCCGAGGTCC TGCTGGGGAT GCCGTACGAT 
CTGGCCATCG ACATGTGGTC TCTAGGCTGC ATCCTGGTGG AGATGCACAC CGGAGAACCG 
CTCTTCAGCG GCGCTAATGA GGTTGATCAG ATGAATAAGA TCGTGGAGGT GTTGGGCGTT 
CCTCCCAGTC ATATCCTGGA GCAGGCACCT AAAGCCAGGA AGTTCTTTGA GAAGATGCCG 
GACAGTATGT GGTGCGCCAA GAAGACCAAA GACGGCAAAA GGTATAAGCC TGCTGGCTCC 
CGGAAGCTGC ACAGTATTCT GGGTGTGGAG GCAGGCGGCC CCGGCGGGCG CAGAGCCGGA 
GAGTCGGGTC ACGCCGTCGC AGACTACCTG AAGTTCAAGG ACCTGATCCT GCGGATGCTA 
GACTATGACC CCAAGACGCG CATCCAGCCG TACTACGCTC TGCAGCACAG CTTCTTCAAG 
AAGACAGCAG ACGAGGGCAC CAACACCAGC AGCAGTGTGT CCACCAGCCC CGCGCTGGAG 
CAGTCGCAGT CCTCCGGGAC CACCTCCAGC ACCTCCTCCA GCTCAGGAGG CTCGTCTGGA 
ACCAGCACCA GTGGCCGCGC CCGCTCAGAC CCCACACATC AGCACCGGCA CAGCGGAGGA 
GGACACTTCA GTGCTGCGGT GGCCATGGAC TGCCACAACC TCTGCCCACA GGCAAGACAG 
GCGTTTGTGC CGGCGCTGGG CTGGGTTGGA GACGGACTCC AGCAGGCGTC AGGAGAGACT 
CACCCCGTAC AGGAGACCAC TTTCCACGTG CCCCCACACC AGCCCAAAGC CCTGCACCCC 
CACCAACACC ACCACCATCA TCACCACCAC CCGCCGCCGC ATGGACAGGG CCCTGCCCGA 
CCTCGACCCC GGCTCTACTC TCCGTCCCAC AGCGCCTCCA CGCAGGACTC AATGGAGGTG 
ACGCACGGAC ACCTGTCCAT GACCTCGCTG TCTTCCTCCG CCTCGTCCTC CTCCACCTCC 
TCCTCCTCCA CCGGCAACCA TCACTACGCC TACCAGAGCC GCCCGCTGCC CCACGGCCTG 
GGCTTCGGCC ACAGCGGCAG CATGACTCTG GGCCTGGGTG CCTTCTCCAA CCCGCGACAG 
GAGACGGGCG TGGCAGGCGT AGGTCTGCGG CCGGGTCTGG AGCGCGAGGA GTCCCCGATG 
GCGGGTGTAT GTGTGCAGCA GAGCTCCGTG GCCAGCTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dyrk1aa ( XM_009305034.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303309.2 Danio rerio dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1A, a (dyrk1aa), transcript variant X1, mRNA.

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CloneID	ODa98401
Clone ID Related Accession (Same CDS sequence)	NM_001347739.1 , XM_009305034.3
Accession Version	XM_009305034.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	dual specificity tyrosine-phosphorylation-regulated kinase 1A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) and transcript sequence (NM_001347739.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009305034.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 27 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGCATACAG GAGGAGAGAC CTCAGCATGC AAACCCTCCT CTGTCCGGCT GGCCCCCTCG 
TTTTCTTTCC ACTCGGCTGG TGTGCAGATG GATGCGCAGA CGCCCCATTC TCACCCGCCG 
TTCAGTGATG CTCACCCACA GGGCGCCGCT GACCAATCCG CTGCTGCGCT GCCCTACAAC 
CCGCAGGCCC CGCAGCCCAG CGCCACCCAG GTAACTGCTG ATATGGTGAT GCTGCAGAGG 
CGGATGCCTC AGTGTTTCCG GGATCCAGCG ACGGCACCCT TAAGAAAACT CTCCATCGAG 
CTGATCAAAA CATACAAACA CATCAATGAG GTTTACTATG CAAAAAAGAA GCGTCGTCAC 
CAACAGGGTC AGGGCGAGGA CTCCAGCAAC AAGAAGGAGA GGAAGATTTA CAACGACGGC 
TATGACGACG ACAACTTCGA CTACATCGTT AAAAATGGCG AGAAGTGGAT GGACCGATAT 
GAGATCGACT CTTTAATAGG GAAAGGCTCG TTCGGGCAGG TGGTGAAGGC GTACGACCGT 
GTGGAGCAGG AGTGGGTCGC CATCAAGATC ATAAAGAATA AAAAGGCCTT CCTGAATCAG 
GCTCAGATCG AAGTGCGACT GCTGGAGCTG ATGAACAAGC ACGACACCGA GATGAAGTAC 
TACATCGTTC ACCTGAAACG CCACTTCATG TTCCGAAACC ACCTGTGTCT GGTGTTCGAG 
ATGCTGTCCT ATAACCTGTA CGATCTGCTG AGGAACACCA ACTTCCGCGG CGTGTCCCTG 
AACCTGACGC GTAAGTTCGC CCAGCAGCTG TGCACCGCGC TGCTGTTCCT GGCCACGCCG 
GAGCTCAGCA TCATCCACTG TGACCTCAAG CCTGAAAACA TCCTGCTCTG CAACCCCAAG 
AGGTCCGCCA TCAAGATCGT GGACTTCGGG AGCTCCTGCC AGCTGGGCCA GCGGATATAC 
CAGTACATCC AGAGTCGCTT CTACCGCTCG CCCGAGGTCC TGCTGGGGAT GCCGTACGAT 
CTGGCCATCG ACATGTGGTC TCTAGGCTGC ATCCTGGTGG AGATGCACAC CGGAGAACCG 
CTCTTCAGCG GCGCTAATGA GGTTGATCAG ATGAATAAGA TCGTGGAGGT GTTGGGCGTT 
CCTCCCAGTC ATATCCTGGA GCAGGCACCT AAAGCCAGGA AGTTCTTTGA GAAGATGCCG 
GACAGTATGT GGTGCGCCAA GAAGACCAAA GACGGCAAAA GGTATAAGCC TGCTGGCTCC 
CGGAAGCTGC ACAGTATTCT GGGTGTGGAG GCAGGCGGCC CCGGCGGGCG CAGAGCCGGA 
GAGTCGGGTC ACGCCGTCGC AGACTACCTG AAGTTCAAGG ACCTGATCCT GCGGATGCTA 
GACTATGACC CCAAGACGCG CATCCAGCCG TACTACGCTC TGCAGCACAG CTTCTTCAAG 
AAGACAGCAG ACGAGGGCAC CAACACCAGC AGCAGTGTGT CCACCAGCCC CGCGCTGGAG 
CAGTCGCAGT CCTCCGGGAC CACCTCCAGC ACCTCCTCCA GCTCAGGAGG CTCGTCTGGA 
ACCAGCACCA GTGGCCGCGC CCGCTCAGAC CCCACACATC AGCACCGGCA CAGCGGAGGA 
GGACACTTCA GTGCTGCGGT GGCCATGGAC TGCCACAACC TCTGCCCACA GGCAAGACAG 
GCGTTTGTGC CGGCGCTGGG CTGGGTTGGA GACGGACTCC AGCAGGCGTC AGGAGAGACT 
CACCCCGTAC AGGAGACCAC TTTCCACGTG CCCCCACACC AGCCCAAAGC CCTGCACCCC 
CACCAACACC ACCACCATCA TCACCACCAC CCGCCGCCGC ATGGACAGGG CCCTGCCCGA 
CCTCGACCCC GGCTCTACTC TCCGTCCCAC AGCGCCTCCA CGCAGGACTC AATGGAGGTG 
ACGCACGGAC ACCTGTCCAT GACCTCGCTG TCTTCCTCCG CCTCGTCCTC CTCCACCTCC 
TCCTCCTCCA CCGGCAACCA TCACTACGCC TACCAGAGCC GCCCGCTGCC CCACGGCCTG 
GGCTTCGGCC ACAGCGGCAG CATGACTCTG GGCCTGGGTG CCTTCTCCAA CCCGCGACAG 
GAGACGGGCG TGGCAGGCGT AGGTCTGCGG CCGGGTCTGG AGCGCGAGGA GTCCCCGATG 
GCGGGTGTAT GTGTGCAGCA GAGCTCCGTG GCCAGCTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303309.2
CDS	583..2784
Translation	MHTGGETSACKPSSVRLAPSFSFHSAGVQMDAQTPHSHPPFSDAHPQGAADQSAAALPYNPQAPQPSATQVTADMVMLQRRMPQCFRDPATAPLRKLSIELIKTYKHINEVYYAKKKRRHQQGQGEDSSNKKERKIYNDGYDDDNFDYIVKNGEKWMDRYEIDSLIGKGSFGQVVKAYDRVEQEWVAIKIIKNKKAFLNQAQIEVRLLELMNKHDTEMKYYIVHLKRHFMFRNHLCLVFEMLSYNLYDLLRNTNFRGVSLNLTRKFAQQLCTALLFLATPELSIIHCDLKPENILLCNPKRSAIKIVDFGSSCQLGQRIYQYIQSRFYRSPEVLLGMPYDLAIDMWSLGCILVEMHTGEPLFSGANEVDQMNKIVEVLGVPPSHILEQAPKARKFFEKMPDSMWCAKKTKDGKRYKPAGSRKLHSILGVEAGGPGGRRAGESGHAVADYLKFKDLILRMLDYDPKTRIQPYYALQHSFFKKTADEGTNTSSSVSTSPALEQSQSSGTTSSTSSSSGGSSGTSTSGRARSDPTHQHRHSGGGHFSAAVAMDCHNLCPQARQAFVPALGWVGDGLQQASGETHPVQETTFHVPPHQPKALHPHQHHHHHHHHPPPHGQGPARPRPRLYSPSHSASTQDSMEVTHGHLSMTSLSSSASSSSTSSSSTGNHHYAYQSRPLPHGLGFGHSGSMTLGLGAFSNPRQETGVAGVGLRPGLEREESPMAGVCVQQSSVASS

Target ORF information:

RefSeq Version	XM_009305034.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1A, a (dyrk1aa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305034.3

ORF Insert Sequence:

ATGCATACAG GAGGAGAGAC CTCAGCATGC AAACCCTCCT CTGTCCGGCT GGCCCCCTCG 
TTTTCTTTCC ACTCGGCTGG TGTGCAGATG GATGCGCAGA CGCCCCATTC TCACCCGCCG 
TTCAGTGATG CTCACCCACA GGGCGCCGCT GACCAATCCG CTGCTGCGCT GCCCTACAAC 
CCGCAGGCCC CGCAGCCCAG CGCCACCCAG GTAACTGCTG ATATGGTGAT GCTGCAGAGG 
CGGATGCCTC AGTGTTTCCG GGATCCAGCG ACGGCACCCT TAAGAAAACT CTCCATCGAG 
CTGATCAAAA CATACAAACA CATCAATGAG GTTTACTATG CAAAAAAGAA GCGTCGTCAC 
CAACAGGGTC AGGGCGAGGA CTCCAGCAAC AAGAAGGAGA GGAAGATTTA CAACGACGGC 
TATGACGACG ACAACTTCGA CTACATCGTT AAAAATGGCG AGAAGTGGAT GGACCGATAT 
GAGATCGACT CTTTAATAGG GAAAGGCTCG TTCGGGCAGG TGGTGAAGGC GTACGACCGT 
GTGGAGCAGG AGTGGGTCGC CATCAAGATC ATAAAGAATA AAAAGGCCTT CCTGAATCAG 
GCTCAGATCG AAGTGCGACT GCTGGAGCTG ATGAACAAGC ACGACACCGA GATGAAGTAC 
TACATCGTTC ACCTGAAACG CCACTTCATG TTCCGAAACC ACCTGTGTCT GGTGTTCGAG 
ATGCTGTCCT ATAACCTGTA CGATCTGCTG AGGAACACCA ACTTCCGCGG CGTGTCCCTG 
AACCTGACGC GTAAGTTCGC CCAGCAGCTG TGCACCGCGC TGCTGTTCCT GGCCACGCCG 
GAGCTCAGCA TCATCCACTG TGACCTCAAG CCTGAAAACA TCCTGCTCTG CAACCCCAAG 
AGGTCCGCCA TCAAGATCGT GGACTTCGGG AGCTCCTGCC AGCTGGGCCA GCGGATATAC 
CAGTACATCC AGAGTCGCTT CTACCGCTCG CCCGAGGTCC TGCTGGGGAT GCCGTACGAT 
CTGGCCATCG ACATGTGGTC TCTAGGCTGC ATCCTGGTGG AGATGCACAC CGGAGAACCG 
CTCTTCAGCG GCGCTAATGA GGTTGATCAG ATGAATAAGA TCGTGGAGGT GTTGGGCGTT 
CCTCCCAGTC ATATCCTGGA GCAGGCACCT AAAGCCAGGA AGTTCTTTGA GAAGATGCCG 
GACAGTATGT GGTGCGCCAA GAAGACCAAA GACGGCAAAA GGTATAAGCC TGCTGGCTCC 
CGGAAGCTGC ACAGTATTCT GGGTGTGGAG GCAGGCGGCC CCGGCGGGCG CAGAGCCGGA 
GAGTCGGGTC ACGCCGTCGC AGACTACCTG AAGTTCAAGG ACCTGATCCT GCGGATGCTA 
GACTATGACC CCAAGACGCG CATCCAGCCG TACTACGCTC TGCAGCACAG CTTCTTCAAG 
AAGACAGCAG ACGAGGGCAC CAACACCAGC AGCAGTGTGT CCACCAGCCC CGCGCTGGAG 
CAGTCGCAGT CCTCCGGGAC CACCTCCAGC ACCTCCTCCA GCTCAGGAGG CTCGTCTGGA 
ACCAGCACCA GTGGCCGCGC CCGCTCAGAC CCCACACATC AGCACCGGCA CAGCGGAGGA 
GGACACTTCA GTGCTGCGGT GGCCATGGAC TGCCACAACC TCTGCCCACA GGCAAGACAG 
GCGTTTGTGC CGGCGCTGGG CTGGGTTGGA GACGGACTCC AGCAGGCGTC AGGAGAGACT 
CACCCCGTAC AGGAGACCAC TTTCCACGTG CCCCCACACC AGCCCAAAGC CCTGCACCCC 
CACCAACACC ACCACCATCA TCACCACCAC CCGCCGCCGC ATGGACAGGG CCCTGCCCGA 
CCTCGACCCC GGCTCTACTC TCCGTCCCAC AGCGCCTCCA CGCAGGACTC AATGGAGGTG 
ACGCACGGAC ACCTGTCCAT GACCTCGCTG TCTTCCTCCG CCTCGTCCTC CTCCACCTCC 
TCCTCCTCCA CCGGCAACCA TCACTACGCC TACCAGAGCC GCCCGCTGCC CCACGGCCTG 
GGCTTCGGCC ACAGCGGCAG CATGACTCTG GGCCTGGGTG CCTTCTCCAA CCCGCGACAG 
GAGACGGGCG TGGCAGGCGT AGGTCTGCGG CCGGGTCTGG AGCGCGAGGA GTCCCCGATG 
GCGGGTGTAT GTGTGCAGCA GAGCTCCGTG GCCAGCTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Overexpression of DYRK1A, a Down Syndrome Candidate gene, Impairs Primordial Germ Cells Maintenance and Migration in zebrafish.
Scientific reports7(1)15313(2017 Nov)
Liu Y,Lin Z,Liu M,Wang H,Sun H

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Zebrafish knockout of Down syndrome gene,
Molecular autism850(2017)
Kim OH,Cho HJ,Han E,Hong TI,Ariyasiri K,Choi JH,Hwang KS,Jeong YM,Yang SY,Yu K,Park DS,Oh HW,Davis EE,Schwartz CE,Lee JS,Kim HG,Kim CH

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dyrk1aa cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

オンラインフォーム