TRPM4A cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	TRPM4A
Entrez Gene ID	100004946
Full Name	transient receptor potential cation channel, subfamily M, member 4a
Synonyms	trpm4
General protein information	Preferred Names transient receptor potential cation channel, subfamily M, member 4a Names transient receptor potential cation channel, subfamily M, member 4a transient receptor potential cation channel, subfamily M, member 4 transient receptor potential melastatin 4a
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005164070.4	XP_005164127.1	transient receptor potential cation channel, subfamily M, member 4a isoform X2
NM_001288815.1	NP_001275744.1	transient receptor potential cation channel, subfamily M, member 4a
XM_021468873.1	XP_021324548.1	transient receptor potential cation channel, subfamily M, member 4a isoform X1
XM_005164070.3	XP_005164127.1	transient receptor potential cation channel, subfamily M, member 4a isoform X1
XM_005164071.4	XP_005164128.1	transient receptor potential cation channel, subfamily M, member 4a isoform X3
NM_001288815.2	NP_001275744.2	transient receptor potential cation channel, subfamily M, member 4a
XM_005164071.3	XP_005164128.1	transient receptor potential cation channel, subfamily M, member 4a isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	TRPM4	NP_060106.2
Canis lupus familiaris (dog)	TRPM4	XP_541500.3
Mus musculus (house mouse)	Trpm4	NP_780339.2
Danio rerio (zebrafish)	LOC100334234	XP_003199613.2
Danio rerio (zebrafish)	LOC100333667	XP_002663943.3
Pan troglodytes (chimpanzee)	TRPM4	XP_512811.2
Bos taurus (cattle)	TRPM4	XP_002695251.2
Rattus norvegicus (Norway rat)	Trpm4	NP_001129701.1
Danio rerio (zebrafish)	trpm4c	XP_001339981.5
	LOC719120	XP_001112814.2
Danio rerio (zebrafish)	trpm4a	XP_003198181.2
Xenopus tropicalis (tropical clawed frog)	trpm5	XP_004916436.1

Gene Ontology
Bibliography

Related articles in PubMed

Thermal stress in Danio rerio: a link between temperature, light, thermo-TRP channels, and clock genes.
Jer?nimo R, Moraes MN, de Assis LVM, Ramos BC, Rocha T, Castrucci AML
Journal of thermal biology68(Pt A)128-138(2017 Aug)

Transcriptomic, proteomic, and metabolomic landscape of positional memory in the caudal fin of zebrafish.
Rabinowitz JS, Robitaille AM, Wang Y, Ray CA, Thummel R, Gu H, Djukovic D, Raftery D, Berndt JD, Moon RT
Proceedings of the National Academy of Sciences of the United States of America114(5)E717-E726(2017 Jan)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Expression and functionality of transient receptor potential melastatin 4 (TRPM4)-like channels during development of the zebrafish.
Cheng H, Ellis J, Kleinow KM
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP17833-44(2015 Dec)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TRPM4A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRPM4A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa08507	XM_005164070.4 Latest version!	Danio rerio transient receptor potential cation channel, subfamily M, member 4a (trpm4a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa08508	XM_005164071.4 Latest version!	Danio rerio transient receptor potential cation channel, subfamily M, member 4a (trpm4a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa159748	NM_001288815.2 Latest version!	Danio rerio transient receptor potential cation channel, subfamily M, member 4a (trpm4a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa47963	XM_021468873.1 Latest version!	Danio rerio transient receptor potential cation channel, subfamily M, member 4a (trpm4a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa35835	NM_001288815.1 Latest version!	Danio rerio transient receptor potential cation channel, subfamily M, member 4a (trpm4a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
ODa08507	XM_005164070.3	Danio rerio transient receptor potential cation channel, subfamily M, member 4a (trpm4a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa08508	XM_005164071.3	Danio rerio transient receptor potential cation channel, subfamily M, member 4a (trpm4a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TRPM4A ( XM_005164070.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005164127.1 Danio rerio transient receptor potential cation channel, subfamily M, member 4a (trpm4a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa08507
Clone ID Related Accession (Same CDS sequence)	XM_005164070.4 , XM_005164070.3
Accession Version	XM_005164070.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2793bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transient receptor potential cation channel, subfamily M, member 4a isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005164070.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCAAAA TGAAGAGAGT CGAGAAAGAG AGTGAAGGCT GTGGGGGAGG AGAGAGAAAA 
GCCCAAAAGG ATCAGAGTTG GATCCCAAAG ATGATTAAAA AGAGAGTGTG CACCACCTTT 
GTGGAGGATT CATGCAGTAA TGGAGCTTTA TGTCAGTGTG GTGGAGCACG GGACCTTCAT 
GTATCTACAA CAGCAGATGA TCATGTAGTC GGCCAATGGG ACAGAGCACG GGACACCTCT 
GATTATCCCA CTGATGCCTT TGGACAGCTG GAGTTTGTTG GAGCCGGACG GAGACACTGT 
CCATTTCTGC GTCTGTCATT CGACACCCCT CCTGATAATG TGTACTCGCT GATGACGTCT 
CAGTGGGGTT TGCCGGTGCC CAATCTAGTG GTTTCCATGG TGGGTGGAGA TGAAAAAGAG 
ATGGCCAAAC CCTGGGTTCG AGAAGTGATC AGACAGGGTC TTGTCCATGC CGCTCAGAGC 
ACAGGGGCTT GGATCATGAC GTCAGGTCTG AAGGAGGGTG TTAGCCGTTG TGTAGGTGAG 
GCCGTCAGGC ATCATTGTGC AATGGCTTCA TCTCTTTCTC AGGCTAAAGT TATTGCCGTT 
GGTGTTGCAC CATGGGGGTT AATTTACCAA CGTCATCAAC TTGTAAACCC ACAGGGTAGT 
TTTCCTGCAC GCTATTATGT GCACGACATG CCCAGTGCTT CGAGCTGTCT GGACAACAAC 
CACCAGGCCT TCCTCTTAGT GGATGATGGA AGTGTGGGTC ACAGAGGCAG TGAGACATCA 
TTCAGAGCCA ACCTGGAGGA CTTCATCTCC CATCAGCGCA CTGGCATCTG GGGAAGTGGA 
AGCATTGAGA TTCCTGTTTT GTGTGTGCTG ATTGCAGGAG ATGCTACAAT GCTCGAGAGA 
GTGGATTTAT CTCTGAAAAA CAGCACTCCA TGGCTGGTGC TGGCAGGGTC GGGGTCTGCA 
TCAGACCTGA TTGCAGGAAT ACTGAAGGAC CTCAGCGCTC CTCTGAGTCC ACCCGTGAGT 
CCCACTTTGC AAAGCGACTC TGAGGAAAGC AGCAAGAGCT CCAGCATTGA GCTCAGGGAC 
CGAGTGAAGG AGAGAATTAG GAAACACTTC CCTTCTGAGA CTGAACTGGA CAAGCTTGTG 
GATCAGGCTC TCAGCATCTA TCATAATAGA GATCTGATCA CTATCTTTCA TGGAGATGAG 
GATGGGTCAG ATGACTTTGA CACAATCATC CTGAAAGCTC TTGTAAGAGC TGCTAAACGT 
GATTCTAATG ATGCAAGCGA GTACACAGAA GAGCTGAAGC TGGCGGTGGC CTGGAACAGA 
GTGGATATTG CCAAGACTGA ACTCTTCAAT GGTGATGTCC ACTGGAGGTA TGAGGACCTG 
GAGGAATCGA TGACTGATGC TCTGGTAAAC GACAAGCCAG ATTTTGTGCG TCTGTTTGTG 
GAAAGCGGCT TAAACATTTT GGCCTATCTG ACGTACCATC GTTTGGAAAG TCTTTACAGC 
TCACTGTCGG ACTGTGATCT GGTGTCCTCA TTCCTCCAAA GGCGGCTGCA GGAGCGAGTG 
GGCTTAGCTG CCTCTCAGAC TCGATTAACT CCTCAACCTG ATGACAATTC CTCTTTCAAG 
AGCCAAGCCA CCAACCTTAC CACACCATCT GCTGCTCGAG ACCTCACTCT GTATGAGGTT 
TCACGAGTGT TATCAGAAAT TATGGAAGAT GTCTGCCAGC CATTTTACTA CACGACTCTT 
GGTTTAGATC ATAGAGTCAA CTCAAGGAAA GCAATGAAGC ATGTGAATAA GTTACTGCTA 
AATGAGAGTA CTTACCGACG GCAGCGTTGT ACATCCCCCT GGATATCTCT GTTCATTTGG 
GCTGTTCTGC AGAATCGCAG AGAAATGTCG CTATACTTCT GGAAAATGAC TGGAGAGTCT 
GTACTAAGTG CTCTTGCCGG GTGTAAGATT TTAAGAGAGC TGTCCAAACT GGAGACAGAG 
ACTGAGACTA AGCTCTCCAT GAAGGAACTT GCTCAAACCT TTGAAAATCT GGCACATGAT 
GTGTTCAGTG AGTGTTACCA GAGCAATGAG AAACGCTCCT TCAAGCTTCT CATCAGACAG 
TCTCCTATAT GGGGTGGTGC CACCTGTCTG CAGATGGCCA CATCGGCAGA CGCACGCCTC 
TTCTTCAGCC ATGATGGAGT TCAGACTTTA TTGTCAGAGA TTTGGTGGGG GGACATGGAC 
AGCAACACAG AGGTGTGGAA ACTGGTCTTC TCATTTTTCC TGCCTCCATT CATCTACACC 
AAGCTCATCA ATTTCAAGGT GGAGGAGGCC GATGAAAAAG CAGAGGACTT TGGTCAGGGT 
AAAGAATTTG ACAGCACTGA TGGAGCCGAG ACATTGGCTG ACATGACGAA CATGGATGAA 
GATGTGGAGG AATGCAATGC TTTAAGAGGA AAACCCAAAG CATCAAAGGT CATCGCTAAG 
AAACGGCCTT TCATATTCCA TCGCTGGCGG CAGTTCTGGT TTGCACCGGT CACTTCATTT 
CTAGGCAATG TGTTGATGTA CTTCCTCTTC CTCTTCCTGT TTGCTTACGT CTTGTTGATT 
GACTTCAAGC CTCCGCCTCC CGATGGACCT GCTCCTTTAG AGCTTGTGTT GTATTTCTGG 
GTTTTTACGC TGGCCTGTGA GGAAATTCGA CAGTCTTTCT TTGTAGGATC TACCACTTTA 
ACTCAAAGGA TGAAGGTGTA CATCCAGGAT ATCTGGAACA AGTGTGACAT CACAGCCCTC 
GGGCTCTTCG TTCTTGGGCT TCTCTGCAGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164127.1
CDS	270..3062
Translation	MIKMKRVEKESEGCGGGERKAQKDQSWIPKMIKKRVCTTFVEDSCSNGALCQCGGARDLHVSTTADDHVVGQWDRARDTSDYPTDAFGQLEFVGAGRRHCPFLRLSFDTPPDNVYSLMTSQWGLPVPNLVVSMVGGDEKEMAKPWVREVIRQGLVHAAQSTGAWIMTSGLKEGVSRCVGEAVRHHCAMASSLSQAKVIAVGVAPWGLIYQRHQLVNPQGSFPARYYVHDMPSASSCLDNNHQAFLLVDDGSVGHRGSETSFRANLEDFISHQRTGIWGSGSIEIPVLCVLIAGDATMLERVDLSLKNSTPWLVLAGSGSASDLIAGILKDLSAPLSPPVSPTLQSDSEESSKSSSIELRDRVKERIRKHFPSETELDKLVDQALSIYHNRDLITIFHGDEDGSDDFDTIILKALVRAAKRDSNDASEYTEELKLAVAWNRVDIAKTELFNGDVHWRYEDLEESMTDALVNDKPDFVRLFVESGLNILAYLTYHRLESLYSSLSDCDLVSSFLQRRLQERVGLAASQTRLTPQPDDNSSFKSQATNLTTPSAARDLTLYEVSRVLSEIMEDVCQPFYYTTLGLDHRVNSRKAMKHVNKLLLNESTYRRQRCTSPWISLFIWAVLQNRREMSLYFWKMTGESVLSALAGCKILRELSKLETETETKLSMKELAQTFENLAHDVFSECYQSNEKRSFKLLIRQSPIWGGATCLQMATSADARLFFSHDGVQTLLSEIWWGDMDSNTEVWKLVFSFFLPPFIYTKLINFKVEEADEKAEDFGQGKEFDSTDGAETLADMTNMDEDVEECNALRGKPKASKVIAKKRPFIFHRWRQFWFAPVTSFLGNVLMYFLFLFLFAYVLLIDFKPPPPDGPAPLELVLYFWVFTLACEEIRQSFFVGSTTLTQRMKVYIQDIWNKCDITALGLFVLGLLCR

Target ORF information:

RefSeq Version	XM_005164070.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily M, member 4a (trpm4a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164070.4

ORF Insert Sequence:

ATGATCAAAA TGAAGAGAGT CGAGAAAGAG AGTGAAGGCT GTGGGGGAGG AGAGAGAAAA 
GCCCAAAAGG ATCAGAGTTG GATCCCAAAG ATGATTAAAA AGAGAGTGTG CACCACCTTT 
GTGGAGGATT CATGCAGTAA TGGAGCTTTA TGTCAGTGTG GTGGAGCACG GGACCTTCAT 
GTATCTACAA CAGCAGATGA TCATGTAGTC GGCCAATGGG ACAGAGCACG GGACACCTCT 
GATTATCCCA CTGATGCCTT TGGACAGCTG GAGTTTGTTG GAGCCGGACG GAGACACTGT 
CCATTTCTGC GTCTGTCATT CGACACCCCT CCTGATAATG TGTACTCGCT GATGACGTCT 
CAGTGGGGTT TGCCGGTGCC CAATCTAGTG GTTTCCATGG TGGGTGGAGA TGAAAAAGAG 
ATGGCCAAAC CCTGGGTTCG AGAAGTGATC AGACAGGGTC TTGTCCATGC CGCTCAGAGC 
ACAGGGGCTT GGATCATGAC GTCAGGTCTG AAGGAGGGTG TTAGCCGTTG TGTAGGTGAG 
GCCGTCAGGC ATCATTGTGC AATGGCTTCA TCTCTTTCTC AGGCTAAAGT TATTGCCGTT 
GGTGTTGCAC CATGGGGGTT AATTTACCAA CGTCATCAAC TTGTAAACCC ACAGGGTAGT 
TTTCCTGCAC GCTATTATGT GCACGACATG CCCAGTGCTT CGAGCTGTCT GGACAACAAC 
CACCAGGCCT TCCTCTTAGT GGATGATGGA AGTGTGGGTC ACAGAGGCAG TGAGACATCA 
TTCAGAGCCA ACCTGGAGGA CTTCATCTCC CATCAGCGCA CTGGCATCTG GGGAAGTGGA 
AGCATTGAGA TTCCTGTTTT GTGTGTGCTG ATTGCAGGAG ATGCTACAAT GCTCGAGAGA 
GTGGATTTAT CTCTGAAAAA CAGCACTCCA TGGCTGGTGC TGGCAGGGTC GGGGTCTGCA 
TCAGACCTGA TTGCAGGAAT ACTGAAGGAC CTCAGCGCTC CTCTGAGTCC ACCCGTGAGT 
CCCACTTTGC AAAGCGACTC TGAGGAAAGC AGCAAGAGCT CCAGCATTGA GCTCAGGGAC 
CGAGTGAAGG AGAGAATTAG GAAACACTTC CCTTCTGAGA CTGAACTGGA CAAGCTTGTG 
GATCAGGCTC TCAGCATCTA TCATAATAGA GATCTGATCA CTATCTTTCA TGGAGATGAG 
GATGGGTCAG ATGACTTTGA CACAATCATC CTGAAAGCTC TTGTAAGAGC TGCTAAACGT 
GATTCTAATG ATGCAAGCGA GTACACAGAA GAGCTGAAGC TGGCGGTGGC CTGGAACAGA 
GTGGATATTG CCAAGACTGA ACTCTTCAAT GGTGATGTCC ACTGGAGGTA TGAGGACCTG 
GAGGAATCGA TGACTGATGC TCTGGTAAAC GACAAGCCAG ATTTTGTGCG TCTGTTTGTG 
GAAAGCGGCT TAAACATTTT GGCCTATCTG ACGTACCATC GTTTGGAAAG TCTTTACAGC 
TCACTGTCGG ACTGTGATCT GGTGTCCTCA TTCCTCCAAA GGCGGCTGCA GGAGCGAGTG 
GGCTTAGCTG CCTCTCAGAC TCGATTAACT CCTCAACCTG ATGACAATTC CTCTTTCAAG 
AGCCAAGCCA CCAACCTTAC CACACCATCT GCTGCTCGAG ACCTCACTCT GTATGAGGTT 
TCACGAGTGT TATCAGAAAT TATGGAAGAT GTCTGCCAGC CATTTTACTA CACGACTCTT 
GGTTTAGATC ATAGAGTCAA CTCAAGGAAA GCAATGAAGC ATGTGAATAA GTTACTGCTA 
AATGAGAGTA CTTACCGACG GCAGCGTTGT ACATCCCCCT GGATATCTCT GTTCATTTGG 
GCTGTTCTGC AGAATCGCAG AGAAATGTCG CTATACTTCT GGAAAATGAC TGGAGAGTCT 
GTACTAAGTG CTCTTGCCGG GTGTAAGATT TTAAGAGAGC TGTCCAAACT GGAGACAGAG 
ACTGAGACTA AGCTCTCCAT GAAGGAACTT GCTCAAACCT TTGAAAATCT GGCACATGAT 
GTGTTCAGTG AGTGTTACCA GAGCAATGAG AAACGCTCCT TCAAGCTTCT CATCAGACAG 
TCTCCTATAT GGGGTGGTGC CACCTGTCTG CAGATGGCCA CATCGGCAGA CGCACGCCTC 
TTCTTCAGCC ATGATGGAGT TCAGACTTTA TTGTCAGAGA TTTGGTGGGG GGACATGGAC 
AGCAACACAG AGGTGTGGAA ACTGGTCTTC TCATTTTTCC TGCCTCCATT CATCTACACC 
AAGCTCATCA ATTTCAAGGT GGAGGAGGCC GATGAAAAAG CAGAGGACTT TGGTCAGGGT 
AAAGAATTTG ACAGCACTGA TGGAGCCGAG ACATTGGCTG ACATGACGAA CATGGATGAA 
GATGTGGAGG AATGCAATGC TTTAAGAGGA AAACCCAAAG CATCAAAGGT CATCGCTAAG 
AAACGGCCTT TCATATTCCA TCGCTGGCGG CAGTTCTGGT TTGCACCGGT CACTTCATTT 
CTAGGCAATG TGTTGATGTA CTTCCTCTTC CTCTTCCTGT TTGCTTACGT CTTGTTGATT 
GACTTCAAGC CTCCGCCTCC CGATGGACCT GCTCCTTTAG AGCTTGTGTT GTATTTCTGG 
GTTTTTACGC TGGCCTGTGA GGAAATTCGA CAGTCTTTCT TTGTAGGATC TACCACTTTA 
ACTCAAAGGA TGAAGGTGTA CATCCAGGAT ATCTGGAACA AGTGTGACAT CACAGCCCTC 
GGGCTCTTCG TTCTTGGGCT TCTCTGCAGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRPM4A ( XM_005164070.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005164127.1 Danio rerio transient receptor potential cation channel, subfamily M, member 4a (trpm4a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa08507
Clone ID Related Accession (Same CDS sequence)	XM_005164070.4 , XM_005164070.3
Accession Version	XM_005164070.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2793bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	transient receptor potential cation channel, subfamily M, member 4a isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005164070.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCAAAA TGAAGAGAGT CGAGAAAGAG AGTGAAGGCT GTGGGGGAGG AGAGAGAAAA 
GCCCAAAAGG ATCAGAGTTG GATCCCAAAG ATGATTAAAA AGAGAGTGTG CACCACCTTT 
GTGGAGGATT CATGCAGTAA TGGAGCTTTA TGTCAGTGTG GTGGAGCACG GGACCTTCAT 
GTATCTACAA CAGCAGATGA TCATGTAGTC GGCCAATGGG ACAGAGCACG GGACACCTCT 
GATTATCCCA CTGATGCCTT TGGACAGCTG GAGTTTGTTG GAGCCGGACG GAGACACTGT 
CCATTTCTGC GTCTGTCATT CGACACCCCT CCTGATAATG TGTACTCGCT GATGACGTCT 
CAGTGGGGTT TGCCGGTGCC CAATCTAGTG GTTTCCATGG TGGGTGGAGA TGAAAAAGAG 
ATGGCCAAAC CCTGGGTTCG AGAAGTGATC AGACAGGGTC TTGTCCATGC CGCTCAGAGC 
ACAGGGGCTT GGATCATGAC GTCAGGTCTG AAGGAGGGTG TTAGCCGTTG TGTAGGTGAG 
GCCGTCAGGC ATCATTGTGC AATGGCTTCA TCTCTTTCTC AGGCTAAAGT TATTGCCGTT 
GGTGTTGCAC CATGGGGGTT AATTTACCAA CGTCATCAAC TTGTAAACCC ACAGGGTAGT 
TTTCCTGCAC GCTATTATGT GCACGACATG CCCAGTGCTT CGAGCTGTCT GGACAACAAC 
CACCAGGCCT TCCTCTTAGT GGATGATGGA AGTGTGGGTC ACAGAGGCAG TGAGACATCA 
TTCAGAGCCA ACCTGGAGGA CTTCATCTCC CATCAGCGCA CTGGCATCTG GGGAAGTGGA 
AGCATTGAGA TTCCTGTTTT GTGTGTGCTG ATTGCAGGAG ATGCTACAAT GCTCGAGAGA 
GTGGATTTAT CTCTGAAAAA CAGCACTCCA TGGCTGGTGC TGGCAGGGTC GGGGTCTGCA 
TCAGACCTGA TTGCAGGAAT ACTGAAGGAC CTCAGCGCTC CTCTGAGTCC ACCCGTGAGT 
CCCACTTTGC AAAGCGACTC TGAGGAAAGC AGCAAGAGCT CCAGCATTGA GCTCAGGGAC 
CGAGTGAAGG AGAGAATTAG GAAACACTTC CCTTCTGAGA CTGAACTGGA CAAGCTTGTG 
GATCAGGCTC TCAGCATCTA TCATAATAGA GATCTGATCA CTATCTTTCA TGGAGATGAG 
GATGGGTCAG ATGACTTTGA CACAATCATC CTGAAAGCTC TTGTAAGAGC TGCTAAACGT 
GATTCTAATG ATGCAAGCGA GTACACAGAA GAGCTGAAGC TGGCGGTGGC CTGGAACAGA 
GTGGATATTG CCAAGACTGA ACTCTTCAAT GGTGATGTCC ACTGGAGGTA TGAGGACCTG 
GAGGAATCGA TGACTGATGC TCTGGTAAAC GACAAGCCAG ATTTTGTGCG TCTGTTTGTG 
GAAAGCGGCT TAAACATTTT GGCCTATCTG ACGTACCATC GTTTGGAAAG TCTTTACAGC 
TCACTGTCGG ACTGTGATCT GGTGTCCTCA TTCCTCCAAA GGCGGCTGCA GGAGCGAGTG 
GGCTTAGCTG CCTCTCAGAC TCGATTAACT CCTCAACCTG ATGACAATTC CTCTTTCAAG 
AGCCAAGCCA CCAACCTTAC CACACCATCT GCTGCTCGAG ACCTCACTCT GTATGAGGTT 
TCACGAGTGT TATCAGAAAT TATGGAAGAT GTCTGCCAGC CATTTTACTA CACGACTCTT 
GGTTTAGATC ATAGAGTCAA CTCAAGGAAA GCAATGAAGC ATGTGAATAA GTTACTGCTA 
AATGAGAGTA CTTACCGACG GCAGCGTTGT ACATCCCCCT GGATATCTCT GTTCATTTGG 
GCTGTTCTGC AGAATCGCAG AGAAATGTCG CTATACTTCT GGAAAATGAC TGGAGAGTCT 
GTACTAAGTG CTCTTGCCGG GTGTAAGATT TTAAGAGAGC TGTCCAAACT GGAGACAGAG 
ACTGAGACTA AGCTCTCCAT GAAGGAACTT GCTCAAACCT TTGAAAATCT GGCACATGAT 
GTGTTCAGTG AGTGTTACCA GAGCAATGAG AAACGCTCCT TCAAGCTTCT CATCAGACAG 
TCTCCTATAT GGGGTGGTGC CACCTGTCTG CAGATGGCCA CATCGGCAGA CGCACGCCTC 
TTCTTCAGCC ATGATGGAGT TCAGACTTTA TTGTCAGAGA TTTGGTGGGG GGACATGGAC 
AGCAACACAG AGGTGTGGAA ACTGGTCTTC TCATTTTTCC TGCCTCCATT CATCTACACC 
AAGCTCATCA ATTTCAAGGT GGAGGAGGCC GATGAAAAAG CAGAGGACTT TGGTCAGGGT 
AAAGAATTTG ACAGCACTGA TGGAGCCGAG ACATTGGCTG ACATGACGAA CATGGATGAA 
GATGTGGAGG AATGCAATGC TTTAAGAGGA AAACCCAAAG CATCAAAGGT CATCGCTAAG 
AAACGGCCTT TCATATTCCA TCGCTGGCGG CAGTTCTGGT TTGCACCGGT CACTTCATTT 
CTAGGCAATG TGTTGATGTA CTTCCTCTTC CTCTTCCTGT TTGCTTACGT CTTGTTGATT 
GACTTCAAGC CTCCGCCTCC CGATGGACCT GCTCCTTTAG AGCTTGTGTT GTATTTCTGG 
GTTTTTACGC TGGCCTGTGA GGAAATTCGA CAGTCTTTCT TTGTAGGATC TACCACTTTA 
ACTCAAAGGA TGAAGGTGTA CATCCAGGAT ATCTGGAACA AGTGTGACAT CACAGCCCTC 
GGGCTCTTCG TTCTTGGGCT TCTCTGCAGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164127.1
CDS	411..3203
Translation	MIKMKRVEKESEGCGGGERKAQKDQSWIPKMIKKRVCTTFVEDSCSNGALCQCGGARDLHVSTTADDHVVGQWDRARDTSDYPTDAFGQLEFVGAGRRHCPFLRLSFDTPPDNVYSLMTSQWGLPVPNLVVSMVGGDEKEMAKPWVREVIRQGLVHAAQSTGAWIMTSGLKEGVSRCVGEAVRHHCAMASSLSQAKVIAVGVAPWGLIYQRHQLVNPQGSFPARYYVHDMPSASSCLDNNHQAFLLVDDGSVGHRGSETSFRANLEDFISHQRTGIWGSGSIEIPVLCVLIAGDATMLERVDLSLKNSTPWLVLAGSGSASDLIAGILKDLSAPLSPPVSPTLQSDSEESSKSSSIELRDRVKERIRKHFPSETELDKLVDQALSIYHNRDLITIFHGDEDGSDDFDTIILKALVRAAKRDSNDASEYTEELKLAVAWNRVDIAKTELFNGDVHWRYEDLEESMTDALVNDKPDFVRLFVESGLNILAYLTYHRLESLYSSLSDCDLVSSFLQRRLQERVGLAASQTRLTPQPDDNSSFKSQATNLTTPSAARDLTLYEVSRVLSEIMEDVCQPFYYTTLGLDHRVNSRKAMKHVNKLLLNESTYRRQRCTSPWISLFIWAVLQNRREMSLYFWKMTGESVLSALAGCKILRELSKLETETETKLSMKELAQTFENLAHDVFSECYQSNEKRSFKLLIRQSPIWGGATCLQMATSADARLFFSHDGVQTLLSEIWWGDMDSNTEVWKLVFSFFLPPFIYTKLINFKVEEADEKAEDFGQGKEFDSTDGAETLADMTNMDEDVEECNALRGKPKASKVIAKKRPFIFHRWRQFWFAPVTSFLGNVLMYFLFLFLFAYVLLIDFKPPPPDGPAPLELVLYFWVFTLACEEIRQSFFVGSTTLTQRMKVYIQDIWNKCDITALGLFVLGLLCR

Target ORF information:

RefSeq Version	XM_005164070.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily M, member 4a (trpm4a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164070.3

ORF Insert Sequence:

ATGATCAAAA TGAAGAGAGT CGAGAAAGAG AGTGAAGGCT GTGGGGGAGG AGAGAGAAAA 
GCCCAAAAGG ATCAGAGTTG GATCCCAAAG ATGATTAAAA AGAGAGTGTG CACCACCTTT 
GTGGAGGATT CATGCAGTAA TGGAGCTTTA TGTCAGTGTG GTGGAGCACG GGACCTTCAT 
GTATCTACAA CAGCAGATGA TCATGTAGTC GGCCAATGGG ACAGAGCACG GGACACCTCT 
GATTATCCCA CTGATGCCTT TGGACAGCTG GAGTTTGTTG GAGCCGGACG GAGACACTGT 
CCATTTCTGC GTCTGTCATT CGACACCCCT CCTGATAATG TGTACTCGCT GATGACGTCT 
CAGTGGGGTT TGCCGGTGCC CAATCTAGTG GTTTCCATGG TGGGTGGAGA TGAAAAAGAG 
ATGGCCAAAC CCTGGGTTCG AGAAGTGATC AGACAGGGTC TTGTCCATGC CGCTCAGAGC 
ACAGGGGCTT GGATCATGAC GTCAGGTCTG AAGGAGGGTG TTAGCCGTTG TGTAGGTGAG 
GCCGTCAGGC ATCATTGTGC AATGGCTTCA TCTCTTTCTC AGGCTAAAGT TATTGCCGTT 
GGTGTTGCAC CATGGGGGTT AATTTACCAA CGTCATCAAC TTGTAAACCC ACAGGGTAGT 
TTTCCTGCAC GCTATTATGT GCACGACATG CCCAGTGCTT CGAGCTGTCT GGACAACAAC 
CACCAGGCCT TCCTCTTAGT GGATGATGGA AGTGTGGGTC ACAGAGGCAG TGAGACATCA 
TTCAGAGCCA ACCTGGAGGA CTTCATCTCC CATCAGCGCA CTGGCATCTG GGGAAGTGGA 
AGCATTGAGA TTCCTGTTTT GTGTGTGCTG ATTGCAGGAG ATGCTACAAT GCTCGAGAGA 
GTGGATTTAT CTCTGAAAAA CAGCACTCCA TGGCTGGTGC TGGCAGGGTC GGGGTCTGCA 
TCAGACCTGA TTGCAGGAAT ACTGAAGGAC CTCAGCGCTC CTCTGAGTCC ACCCGTGAGT 
CCCACTTTGC AAAGCGACTC TGAGGAAAGC AGCAAGAGCT CCAGCATTGA GCTCAGGGAC 
CGAGTGAAGG AGAGAATTAG GAAACACTTC CCTTCTGAGA CTGAACTGGA CAAGCTTGTG 
GATCAGGCTC TCAGCATCTA TCATAATAGA GATCTGATCA CTATCTTTCA TGGAGATGAG 
GATGGGTCAG ATGACTTTGA CACAATCATC CTGAAAGCTC TTGTAAGAGC TGCTAAACGT 
GATTCTAATG ATGCAAGCGA GTACACAGAA GAGCTGAAGC TGGCGGTGGC CTGGAACAGA 
GTGGATATTG CCAAGACTGA ACTCTTCAAT GGTGATGTCC ACTGGAGGTA TGAGGACCTG 
GAGGAATCGA TGACTGATGC TCTGGTAAAC GACAAGCCAG ATTTTGTGCG TCTGTTTGTG 
GAAAGCGGCT TAAACATTTT GGCCTATCTG ACGTACCATC GTTTGGAAAG TCTTTACAGC 
TCACTGTCGG ACTGTGATCT GGTGTCCTCA TTCCTCCAAA GGCGGCTGCA GGAGCGAGTG 
GGCTTAGCTG CCTCTCAGAC TCGATTAACT CCTCAACCTG ATGACAATTC CTCTTTCAAG 
AGCCAAGCCA CCAACCTTAC CACACCATCT GCTGCTCGAG ACCTCACTCT GTATGAGGTT 
TCACGAGTGT TATCAGAAAT TATGGAAGAT GTCTGCCAGC CATTTTACTA CACGACTCTT 
GGTTTAGATC ATAGAGTCAA CTCAAGGAAA GCAATGAAGC ATGTGAATAA GTTACTGCTA 
AATGAGAGTA CTTACCGACG GCAGCGTTGT ACATCCCCCT GGATATCTCT GTTCATTTGG 
GCTGTTCTGC AGAATCGCAG AGAAATGTCG CTATACTTCT GGAAAATGAC TGGAGAGTCT 
GTACTAAGTG CTCTTGCCGG GTGTAAGATT TTAAGAGAGC TGTCCAAACT GGAGACAGAG 
ACTGAGACTA AGCTCTCCAT GAAGGAACTT GCTCAAACCT TTGAAAATCT GGCACATGAT 
GTGTTCAGTG AGTGTTACCA GAGCAATGAG AAACGCTCCT TCAAGCTTCT CATCAGACAG 
TCTCCTATAT GGGGTGGTGC CACCTGTCTG CAGATGGCCA CATCGGCAGA CGCACGCCTC 
TTCTTCAGCC ATGATGGAGT TCAGACTTTA TTGTCAGAGA TTTGGTGGGG GGACATGGAC 
AGCAACACAG AGGTGTGGAA ACTGGTCTTC TCATTTTTCC TGCCTCCATT CATCTACACC 
AAGCTCATCA ATTTCAAGGT GGAGGAGGCC GATGAAAAAG CAGAGGACTT TGGTCAGGGT 
AAAGAATTTG ACAGCACTGA TGGAGCCGAG ACATTGGCTG ACATGACGAA CATGGATGAA 
GATGTGGAGG AATGCAATGC TTTAAGAGGA AAACCCAAAG CATCAAAGGT CATCGCTAAG 
AAACGGCCTT TCATATTCCA TCGCTGGCGG CAGTTCTGGT TTGCACCGGT CACTTCATTT 
CTAGGCAATG TGTTGATGTA CTTCCTCTTC CTCTTCCTGT TTGCTTACGT CTTGTTGATT 
GACTTCAAGC CTCCGCCTCC CGATGGACCT GCTCCTTTAG AGCTTGTGTT GTATTTCTGG 
GTTTTTACGC TGGCCTGTGA GGAAATTCGA CAGTCTTTCT TTGTAGGATC TACCACTTTA 
ACTCAAAGGA TGAAGGTGTA CATCCAGGAT ATCTGGAACA AGTGTGACAT CACAGCCCTC 
GGGCTCTTCG TTCTTGGGCT TCTCTGCAGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRPM4A ( XM_005164071.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005164128.1 Danio rerio transient receptor potential cation channel, subfamily M, member 4a (trpm4a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa08508
Clone ID Related Accession (Same CDS sequence)	XM_005164071.4 , XM_005164071.3
Accession Version	XM_005164071.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transient receptor potential cation channel, subfamily M, member 4a isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005164071.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTGTC TTTACAGCTC ACTGTCGGAC TGTGATCTGG TGTCCTCATT CCTCCAAAGG 
CGGCTGCAGG AGCGAGTGGG CTTAGCTGCC TCTCAGACTC GATTAACTCC TCAACCTGAT 
GACAATTCCT CTTTCAAGAG CCAAGCCACC AACCTTACCA CACCATCTGC TGCTCGAGAC 
CTCACTCTGT ATGAGGTTTC ACGAGTGTTA TCAGAAATTA TGGAAGATGT CTGCCAGCCA 
TTTTACTACA CGACTCTTGG TTTAGATCAT AGAGTCAACT CAAGGAAAGC AATGAAGCAT 
GTGAATAAGT TACTGCTAAA TGAGAGTACT TACCGACGGC AGCGTTGTAC ATCCCCCTGG 
ATATCTCTGT TCATTTGGGC TGTTCTGCAG AATCGCAGAG AAATGTCGCT ATACTTCTGG 
AAAATGACTG GAGAGTCTGT ACTAAGTGCT CTTGCCGGGT GTAAGATTTT AAGAGAGCTG 
TCCAAACTGG AGACAGAGAC TGAGACTAAG CTCTCCATGA AGGAACTTGC TCAAACCTTT 
GAAAATCTGG CACATGATGT GTTCAGTGAG TGTTACCAGA GCAATGAGAA ACGCTCCTTC 
AAGCTTCTCA TCAGACAGTC TCCTATATGG GGTGGTGCCA CCTGTCTGCA GATGGCCACA 
TCGGCAGACG CACGCCTCTT CTTCAGCCAT GATGGAGTTC AGACTTTATT GTCAGAGATT 
TGGTGGGGGG ACATGGACAG CAACACAGAG GTGTGGAAAC TGGTCTTCTC ATTTTTCCTG 
CCTCCATTCA TCTACACCAA GCTCATCAAT TTCAAGGTGG AGGAGGCCGA TGAAAAAGCA 
GAGGACTTTG GTCAGGGTAA AGAATTTGAC AGCACTGATG GAGCCGAGAC ATTGGCTGAC 
ATGACGAACA TGGATGAAGA TGTGGAGGAA TGCAATGCTT TAAGAGGAAA ACCCAAAGCA 
TCAAAGGTCA TCGCTAAGAA ACGGCCTTTC ATATTCCATC GCTGGCGGCA GTTCTGGTTT 
GCACCGGTCA CTTCATTTCT AGGCAATGTG TTGATGTACT TCCTCTTCCT CTTCCTGTTT 
GCTTACGTCT TGTTGATTGA CTTCAAGCCT CCGCCTCCCG ATGGACCTGC TCCTTTAGAG 
CTTGTGTTGT ATTTCTGGGT TTTTACGCTG GCCTGTGAGG AAATTCGACA GTCTTTCTTT 
GTAGGATCTA CCACTTTAAC TCAAAGGATG AAGGTGTACA TCCAGGATAT CTGGAACAAG 
TGTGACATCA CAGCCCTCGG GCTCTTCGTT CTTGGGCTTC TCTGCAGGAT GTTTCCATGG 
ACATATAATT TCGGTCGTGC AGTTATGTGT GTTGACTATA CAGTCTTCAC ACTCAGACTT 
ATTCATATAT TTGCAGTTCA CAAACAACTT GGTCCAAAAA TCATTATAGT TGGCAAAATG 
GTGAAAGATG TGTTCTTCTT CCTGTTCTTC CTGGGGGTGT GGCTGATGGC ATATGGAGTG 
GCCAATCAGG CTCTGCTGTA CTCCTATGAC TCTCGCCCTG GTTGGGTAGC TCGGCGTGTG 
TTCTACAGGC CATATATGCA CATATACGGG CAGATACCAC TGGATGAAAT TGATGCTGAT 
AAGCGACACG ACAAACGGTG TACGGATAAT GTCACTCTCA TCCACGAGGG GGCAGAGCCC 
TGCCCAGACT CTGATGCTAA TTGGCTTGTC CTTATTCTAC TGTCAGTCTA CTTGCTGGTC 
ACCAATATTC TGTTGGTGAA CTTGCTCATT GCTATGTTCA GTTACACATT CAATAAAGTC 
CAGGAGCGCA GTGATGTCTA CTGGAAGTTC CAGCGCTACA ACCTGATTGT GGAGTACCAC 
TCGCGCCCCT GCCTCGCCCC GCCCTTCATC ATCATCTCCC ACCTGCATAT CTTCATCAAG 
AGGGTCATTC AGAGATTGCC TTCCCTTAAG AGCCAACACT TCATGTTGGA CCTGACAAGC 
AAAGAGACGA GCTTGTTGAA CACATGGGAA TTGTCACTGA AAGAGAACCT GCTGTCCACG 
GAGAGCAAGA AGAAAAGAGA GAGCGACACG GAGCGACTCA AACGCACCTC CACAAAGGTG 
GACAGCGTCT TGAAGCAGAT GGTGGAGATT CGTGATCACG ATCGCAGGTT GAGAAAGCTG 
GAGTCTGAGG TGGAGTACTG CTCTACTGCC CTCTCCTGGA TAGTGGAGGC ACTGTCCCAG 
AGTGATCTTG TGAAGCAGAC TCGTCCACCT CCTTATCCTT CCAAAGGTAT TGCTTCAAAA 
CGTCCCACAT TTTCCAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164128.1
CDS	105..2405
Translation	MLCLYSSLSDCDLVSSFLQRRLQERVGLAASQTRLTPQPDDNSSFKSQATNLTTPSAARDLTLYEVSRVLSEIMEDVCQPFYYTTLGLDHRVNSRKAMKHVNKLLLNESTYRRQRCTSPWISLFIWAVLQNRREMSLYFWKMTGESVLSALAGCKILRELSKLETETETKLSMKELAQTFENLAHDVFSECYQSNEKRSFKLLIRQSPIWGGATCLQMATSADARLFFSHDGVQTLLSEIWWGDMDSNTEVWKLVFSFFLPPFIYTKLINFKVEEADEKAEDFGQGKEFDSTDGAETLADMTNMDEDVEECNALRGKPKASKVIAKKRPFIFHRWRQFWFAPVTSFLGNVLMYFLFLFLFAYVLLIDFKPPPPDGPAPLELVLYFWVFTLACEEIRQSFFVGSTTLTQRMKVYIQDIWNKCDITALGLFVLGLLCRMFPWTYNFGRAVMCVDYTVFTLRLIHIFAVHKQLGPKIIIVGKMVKDVFFFLFFLGVWLMAYGVANQALLYSYDSRPGWVARRVFYRPYMHIYGQIPLDEIDADKRHDKRCTDNVTLIHEGAEPCPDSDANWLVLILLSVYLLVTNILLVNLLIAMFSYTFNKVQERSDVYWKFQRYNLIVEYHSRPCLAPPFIIISHLHIFIKRVIQRLPSLKSQHFMLDLTSKETSLLNTWELSLKENLLSTESKKKRESDTERLKRTSTKVDSVLKQMVEIRDHDRRLRKLESEVEYCSTALSWIVEALSQSDLVKQTRPPPYPSKGIASKRPTFSR

Target ORF information:

RefSeq Version	XM_005164071.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily M, member 4a (trpm4a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164071.4

ORF Insert Sequence:

ATGCTCTGTC TTTACAGCTC ACTGTCGGAC TGTGATCTGG TGTCCTCATT CCTCCAAAGG 
CGGCTGCAGG AGCGAGTGGG CTTAGCTGCC TCTCAGACTC GATTAACTCC TCAACCTGAT 
GACAATTCCT CTTTCAAGAG CCAAGCCACC AACCTTACCA CACCATCTGC TGCTCGAGAC 
CTCACTCTGT ATGAGGTTTC ACGAGTGTTA TCAGAAATTA TGGAAGATGT CTGCCAGCCA 
TTTTACTACA CGACTCTTGG TTTAGATCAT AGAGTCAACT CAAGGAAAGC AATGAAGCAT 
GTGAATAAGT TACTGCTAAA TGAGAGTACT TACCGACGGC AGCGTTGTAC ATCCCCCTGG 
ATATCTCTGT TCATTTGGGC TGTTCTGCAG AATCGCAGAG AAATGTCGCT ATACTTCTGG 
AAAATGACTG GAGAGTCTGT ACTAAGTGCT CTTGCCGGGT GTAAGATTTT AAGAGAGCTG 
TCCAAACTGG AGACAGAGAC TGAGACTAAG CTCTCCATGA AGGAACTTGC TCAAACCTTT 
GAAAATCTGG CACATGATGT GTTCAGTGAG TGTTACCAGA GCAATGAGAA ACGCTCCTTC 
AAGCTTCTCA TCAGACAGTC TCCTATATGG GGTGGTGCCA CCTGTCTGCA GATGGCCACA 
TCGGCAGACG CACGCCTCTT CTTCAGCCAT GATGGAGTTC AGACTTTATT GTCAGAGATT 
TGGTGGGGGG ACATGGACAG CAACACAGAG GTGTGGAAAC TGGTCTTCTC ATTTTTCCTG 
CCTCCATTCA TCTACACCAA GCTCATCAAT TTCAAGGTGG AGGAGGCCGA TGAAAAAGCA 
GAGGACTTTG GTCAGGGTAA AGAATTTGAC AGCACTGATG GAGCCGAGAC ATTGGCTGAC 
ATGACGAACA TGGATGAAGA TGTGGAGGAA TGCAATGCTT TAAGAGGAAA ACCCAAAGCA 
TCAAAGGTCA TCGCTAAGAA ACGGCCTTTC ATATTCCATC GCTGGCGGCA GTTCTGGTTT 
GCACCGGTCA CTTCATTTCT AGGCAATGTG TTGATGTACT TCCTCTTCCT CTTCCTGTTT 
GCTTACGTCT TGTTGATTGA CTTCAAGCCT CCGCCTCCCG ATGGACCTGC TCCTTTAGAG 
CTTGTGTTGT ATTTCTGGGT TTTTACGCTG GCCTGTGAGG AAATTCGACA GTCTTTCTTT 
GTAGGATCTA CCACTTTAAC TCAAAGGATG AAGGTGTACA TCCAGGATAT CTGGAACAAG 
TGTGACATCA CAGCCCTCGG GCTCTTCGTT CTTGGGCTTC TCTGCAGGAT GTTTCCATGG 
ACATATAATT TCGGTCGTGC AGTTATGTGT GTTGACTATA CAGTCTTCAC ACTCAGACTT 
ATTCATATAT TTGCAGTTCA CAAACAACTT GGTCCAAAAA TCATTATAGT TGGCAAAATG 
GTGAAAGATG TGTTCTTCTT CCTGTTCTTC CTGGGGGTGT GGCTGATGGC ATATGGAGTG 
GCCAATCAGG CTCTGCTGTA CTCCTATGAC TCTCGCCCTG GTTGGGTAGC TCGGCGTGTG 
TTCTACAGGC CATATATGCA CATATACGGG CAGATACCAC TGGATGAAAT TGATGCTGAT 
AAGCGACACG ACAAACGGTG TACGGATAAT GTCACTCTCA TCCACGAGGG GGCAGAGCCC 
TGCCCAGACT CTGATGCTAA TTGGCTTGTC CTTATTCTAC TGTCAGTCTA CTTGCTGGTC 
ACCAATATTC TGTTGGTGAA CTTGCTCATT GCTATGTTCA GTTACACATT CAATAAAGTC 
CAGGAGCGCA GTGATGTCTA CTGGAAGTTC CAGCGCTACA ACCTGATTGT GGAGTACCAC 
TCGCGCCCCT GCCTCGCCCC GCCCTTCATC ATCATCTCCC ACCTGCATAT CTTCATCAAG 
AGGGTCATTC AGAGATTGCC TTCCCTTAAG AGCCAACACT TCATGTTGGA CCTGACAAGC 
AAAGAGACGA GCTTGTTGAA CACATGGGAA TTGTCACTGA AAGAGAACCT GCTGTCCACG 
GAGAGCAAGA AGAAAAGAGA GAGCGACACG GAGCGACTCA AACGCACCTC CACAAAGGTG 
GACAGCGTCT TGAAGCAGAT GGTGGAGATT CGTGATCACG ATCGCAGGTT GAGAAAGCTG 
GAGTCTGAGG TGGAGTACTG CTCTACTGCC CTCTCCTGGA TAGTGGAGGC ACTGTCCCAG 
AGTGATCTTG TGAAGCAGAC TCGTCCACCT CCTTATCCTT CCAAAGGTAT TGCTTCAAAA 
CGTCCCACAT TTTCCAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRPM4A ( XM_005164071.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005164128.1 Danio rerio transient receptor potential cation channel, subfamily M, member 4a (trpm4a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa08508
Clone ID Related Accession (Same CDS sequence)	XM_005164071.4 , XM_005164071.3
Accession Version	XM_005164071.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	transient receptor potential cation channel, subfamily M, member 4a isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005164071.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTGTC TTTACAGCTC ACTGTCGGAC TGTGATCTGG TGTCCTCATT CCTCCAAAGG 
CGGCTGCAGG AGCGAGTGGG CTTAGCTGCC TCTCAGACTC GATTAACTCC TCAACCTGAT 
GACAATTCCT CTTTCAAGAG CCAAGCCACC AACCTTACCA CACCATCTGC TGCTCGAGAC 
CTCACTCTGT ATGAGGTTTC ACGAGTGTTA TCAGAAATTA TGGAAGATGT CTGCCAGCCA 
TTTTACTACA CGACTCTTGG TTTAGATCAT AGAGTCAACT CAAGGAAAGC AATGAAGCAT 
GTGAATAAGT TACTGCTAAA TGAGAGTACT TACCGACGGC AGCGTTGTAC ATCCCCCTGG 
ATATCTCTGT TCATTTGGGC TGTTCTGCAG AATCGCAGAG AAATGTCGCT ATACTTCTGG 
AAAATGACTG GAGAGTCTGT ACTAAGTGCT CTTGCCGGGT GTAAGATTTT AAGAGAGCTG 
TCCAAACTGG AGACAGAGAC TGAGACTAAG CTCTCCATGA AGGAACTTGC TCAAACCTTT 
GAAAATCTGG CACATGATGT GTTCAGTGAG TGTTACCAGA GCAATGAGAA ACGCTCCTTC 
AAGCTTCTCA TCAGACAGTC TCCTATATGG GGTGGTGCCA CCTGTCTGCA GATGGCCACA 
TCGGCAGACG CACGCCTCTT CTTCAGCCAT GATGGAGTTC AGACTTTATT GTCAGAGATT 
TGGTGGGGGG ACATGGACAG CAACACAGAG GTGTGGAAAC TGGTCTTCTC ATTTTTCCTG 
CCTCCATTCA TCTACACCAA GCTCATCAAT TTCAAGGTGG AGGAGGCCGA TGAAAAAGCA 
GAGGACTTTG GTCAGGGTAA AGAATTTGAC AGCACTGATG GAGCCGAGAC ATTGGCTGAC 
ATGACGAACA TGGATGAAGA TGTGGAGGAA TGCAATGCTT TAAGAGGAAA ACCCAAAGCA 
TCAAAGGTCA TCGCTAAGAA ACGGCCTTTC ATATTCCATC GCTGGCGGCA GTTCTGGTTT 
GCACCGGTCA CTTCATTTCT AGGCAATGTG TTGATGTACT TCCTCTTCCT CTTCCTGTTT 
GCTTACGTCT TGTTGATTGA CTTCAAGCCT CCGCCTCCCG ATGGACCTGC TCCTTTAGAG 
CTTGTGTTGT ATTTCTGGGT TTTTACGCTG GCCTGTGAGG AAATTCGACA GTCTTTCTTT 
GTAGGATCTA CCACTTTAAC TCAAAGGATG AAGGTGTACA TCCAGGATAT CTGGAACAAG 
TGTGACATCA CAGCCCTCGG GCTCTTCGTT CTTGGGCTTC TCTGCAGGAT GTTTCCATGG 
ACATATAATT TCGGTCGTGC AGTTATGTGT GTTGACTATA CAGTCTTCAC ACTCAGACTT 
ATTCATATAT TTGCAGTTCA CAAACAACTT GGTCCAAAAA TCATTATAGT TGGCAAAATG 
GTGAAAGATG TGTTCTTCTT CCTGTTCTTC CTGGGGGTGT GGCTGATGGC ATATGGAGTG 
GCCAATCAGG CTCTGCTGTA CTCCTATGAC TCTCGCCCTG GTTGGGTAGC TCGGCGTGTG 
TTCTACAGGC CATATATGCA CATATACGGG CAGATACCAC TGGATGAAAT TGATGCTGAT 
AAGCGACACG ACAAACGGTG TACGGATAAT GTCACTCTCA TCCACGAGGG GGCAGAGCCC 
TGCCCAGACT CTGATGCTAA TTGGCTTGTC CTTATTCTAC TGTCAGTCTA CTTGCTGGTC 
ACCAATATTC TGTTGGTGAA CTTGCTCATT GCTATGTTCA GTTACACATT CAATAAAGTC 
CAGGAGCGCA GTGATGTCTA CTGGAAGTTC CAGCGCTACA ACCTGATTGT GGAGTACCAC 
TCGCGCCCCT GCCTCGCCCC GCCCTTCATC ATCATCTCCC ACCTGCATAT CTTCATCAAG 
AGGGTCATTC AGAGATTGCC TTCCCTTAAG AGCCAACACT TCATGTTGGA CCTGACAAGC 
AAAGAGACGA GCTTGTTGAA CACATGGGAA TTGTCACTGA AAGAGAACCT GCTGTCCACG 
GAGAGCAAGA AGAAAAGAGA GAGCGACACG GAGCGACTCA AACGCACCTC CACAAAGGTG 
GACAGCGTCT TGAAGCAGAT GGTGGAGATT CGTGATCACG ATCGCAGGTT GAGAAAGCTG 
GAGTCTGAGG TGGAGTACTG CTCTACTGCC CTCTCCTGGA TAGTGGAGGC ACTGTCCCAG 
AGTGATCTTG TGAAGCAGAC TCGTCCACCT CCTTATCCTT CCAAAGGTAT TGCTTCAAAA 
CGTCCCACAT TTTCCAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164128.1
CDS	29..2329
Translation	MLCLYSSLSDCDLVSSFLQRRLQERVGLAASQTRLTPQPDDNSSFKSQATNLTTPSAARDLTLYEVSRVLSEIMEDVCQPFYYTTLGLDHRVNSRKAMKHVNKLLLNESTYRRQRCTSPWISLFIWAVLQNRREMSLYFWKMTGESVLSALAGCKILRELSKLETETETKLSMKELAQTFENLAHDVFSECYQSNEKRSFKLLIRQSPIWGGATCLQMATSADARLFFSHDGVQTLLSEIWWGDMDSNTEVWKLVFSFFLPPFIYTKLINFKVEEADEKAEDFGQGKEFDSTDGAETLADMTNMDEDVEECNALRGKPKASKVIAKKRPFIFHRWRQFWFAPVTSFLGNVLMYFLFLFLFAYVLLIDFKPPPPDGPAPLELVLYFWVFTLACEEIRQSFFVGSTTLTQRMKVYIQDIWNKCDITALGLFVLGLLCRMFPWTYNFGRAVMCVDYTVFTLRLIHIFAVHKQLGPKIIIVGKMVKDVFFFLFFLGVWLMAYGVANQALLYSYDSRPGWVARRVFYRPYMHIYGQIPLDEIDADKRHDKRCTDNVTLIHEGAEPCPDSDANWLVLILLSVYLLVTNILLVNLLIAMFSYTFNKVQERSDVYWKFQRYNLIVEYHSRPCLAPPFIIISHLHIFIKRVIQRLPSLKSQHFMLDLTSKETSLLNTWELSLKENLLSTESKKKRESDTERLKRTSTKVDSVLKQMVEIRDHDRRLRKLESEVEYCSTALSWIVEALSQSDLVKQTRPPPYPSKGIASKRPTFSR

Target ORF information:

RefSeq Version	XM_005164071.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily M, member 4a (trpm4a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164071.3

ORF Insert Sequence:

ATGCTCTGTC TTTACAGCTC ACTGTCGGAC TGTGATCTGG TGTCCTCATT CCTCCAAAGG 
CGGCTGCAGG AGCGAGTGGG CTTAGCTGCC TCTCAGACTC GATTAACTCC TCAACCTGAT 
GACAATTCCT CTTTCAAGAG CCAAGCCACC AACCTTACCA CACCATCTGC TGCTCGAGAC 
CTCACTCTGT ATGAGGTTTC ACGAGTGTTA TCAGAAATTA TGGAAGATGT CTGCCAGCCA 
TTTTACTACA CGACTCTTGG TTTAGATCAT AGAGTCAACT CAAGGAAAGC AATGAAGCAT 
GTGAATAAGT TACTGCTAAA TGAGAGTACT TACCGACGGC AGCGTTGTAC ATCCCCCTGG 
ATATCTCTGT TCATTTGGGC TGTTCTGCAG AATCGCAGAG AAATGTCGCT ATACTTCTGG 
AAAATGACTG GAGAGTCTGT ACTAAGTGCT CTTGCCGGGT GTAAGATTTT AAGAGAGCTG 
TCCAAACTGG AGACAGAGAC TGAGACTAAG CTCTCCATGA AGGAACTTGC TCAAACCTTT 
GAAAATCTGG CACATGATGT GTTCAGTGAG TGTTACCAGA GCAATGAGAA ACGCTCCTTC 
AAGCTTCTCA TCAGACAGTC TCCTATATGG GGTGGTGCCA CCTGTCTGCA GATGGCCACA 
TCGGCAGACG CACGCCTCTT CTTCAGCCAT GATGGAGTTC AGACTTTATT GTCAGAGATT 
TGGTGGGGGG ACATGGACAG CAACACAGAG GTGTGGAAAC TGGTCTTCTC ATTTTTCCTG 
CCTCCATTCA TCTACACCAA GCTCATCAAT TTCAAGGTGG AGGAGGCCGA TGAAAAAGCA 
GAGGACTTTG GTCAGGGTAA AGAATTTGAC AGCACTGATG GAGCCGAGAC ATTGGCTGAC 
ATGACGAACA TGGATGAAGA TGTGGAGGAA TGCAATGCTT TAAGAGGAAA ACCCAAAGCA 
TCAAAGGTCA TCGCTAAGAA ACGGCCTTTC ATATTCCATC GCTGGCGGCA GTTCTGGTTT 
GCACCGGTCA CTTCATTTCT AGGCAATGTG TTGATGTACT TCCTCTTCCT CTTCCTGTTT 
GCTTACGTCT TGTTGATTGA CTTCAAGCCT CCGCCTCCCG ATGGACCTGC TCCTTTAGAG 
CTTGTGTTGT ATTTCTGGGT TTTTACGCTG GCCTGTGAGG AAATTCGACA GTCTTTCTTT 
GTAGGATCTA CCACTTTAAC TCAAAGGATG AAGGTGTACA TCCAGGATAT CTGGAACAAG 
TGTGACATCA CAGCCCTCGG GCTCTTCGTT CTTGGGCTTC TCTGCAGGAT GTTTCCATGG 
ACATATAATT TCGGTCGTGC AGTTATGTGT GTTGACTATA CAGTCTTCAC ACTCAGACTT 
ATTCATATAT TTGCAGTTCA CAAACAACTT GGTCCAAAAA TCATTATAGT TGGCAAAATG 
GTGAAAGATG TGTTCTTCTT CCTGTTCTTC CTGGGGGTGT GGCTGATGGC ATATGGAGTG 
GCCAATCAGG CTCTGCTGTA CTCCTATGAC TCTCGCCCTG GTTGGGTAGC TCGGCGTGTG 
TTCTACAGGC CATATATGCA CATATACGGG CAGATACCAC TGGATGAAAT TGATGCTGAT 
AAGCGACACG ACAAACGGTG TACGGATAAT GTCACTCTCA TCCACGAGGG GGCAGAGCCC 
TGCCCAGACT CTGATGCTAA TTGGCTTGTC CTTATTCTAC TGTCAGTCTA CTTGCTGGTC 
ACCAATATTC TGTTGGTGAA CTTGCTCATT GCTATGTTCA GTTACACATT CAATAAAGTC 
CAGGAGCGCA GTGATGTCTA CTGGAAGTTC CAGCGCTACA ACCTGATTGT GGAGTACCAC 
TCGCGCCCCT GCCTCGCCCC GCCCTTCATC ATCATCTCCC ACCTGCATAT CTTCATCAAG 
AGGGTCATTC AGAGATTGCC TTCCCTTAAG AGCCAACACT TCATGTTGGA CCTGACAAGC 
AAAGAGACGA GCTTGTTGAA CACATGGGAA TTGTCACTGA AAGAGAACCT GCTGTCCACG 
GAGAGCAAGA AGAAAAGAGA GAGCGACACG GAGCGACTCA AACGCACCTC CACAAAGGTG 
GACAGCGTCT TGAAGCAGAT GGTGGAGATT CGTGATCACG ATCGCAGGTT GAGAAAGCTG 
GAGTCTGAGG TGGAGTACTG CTCTACTGCC CTCTCCTGGA TAGTGGAGGC ACTGTCCCAG 
AGTGATCTTG TGAAGCAGAC TCGTCCACCT CCTTATCCTT CCAAAGGTAT TGCTTCAAAA 
CGTCCCACAT TTTCCAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRPM4A ( NM_001288815.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001275744.2 Danio rerio transient receptor potential cation channel, subfamily M, member 4a (trpm4a), mRNA.

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CloneID	ODa159748
Clone ID Related Accession (Same CDS sequence)	NM_001288815.2
Accession Version	NM_001288815.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-21
Organism	Danio rerio(zebrafish)
Product	transient receptor potential cation channel, subfamily M, member 4a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FP236460.5 and FP236454.4. On Sep 12, 2018 this sequence version replaced NM_001288815.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: KF305309.1, GFIL01015186.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGATCAAAA TGAAGAGAGT CGAGAAAGAG AGTGAAGGCT GTGGGGGAGG AGAGAGAAAA 
GCCCAAAAGG ATCAGAGTTG GATCCCAAAG ATGATTAAAA AGAGAGTGTG CACCACCTTT 
GTGGAGGATT CATGCAGTAA TGGAGCTTTA TGTCAGTGTG GTGGAGCACG GGACCTTCAT 
GTATCTACAA CAGCAGATGA TCATGTAGTC GGCCAATGGG ACAGAGCACG GGACACCTCT 
GATTATCCCA CTGATGCCTT TGGACAGCTG GAGTTTGTTG GAGCCGGACG GAGACACTGT 
CCATTTCTGC GTCTGTCATT CGACACCCCT CCTGATAATG TGTACTCGCT GATGACGTCT 
CAGTGGGGTT TGCCGGTGCC CAATCTAGTG GTTTCCATGG TGGGTGGAGA TGAAAAAGAG 
ATGGCCAAAC CCTGGGTTCG AGAAGTGATC AGACAGGGTC TTGTCCATGC CGCTCAGAGC 
ACAGGGGCTT GGATCATGAC GTCAGGTCTG AAGGAGGGTG TTAGCCGTTG TGTAGGTGAG 
GCCGTCAGGC ATCATTGTGC AATGGCTTCA TCTCTTTCTC AGGCTAAAGT TATTGCCGTT 
GGTGTTGCAC CATGGGGGTT AATTTACCAA CGTCATCAAC TTGTAAACCC ACAGGGTAGT 
TTTCCTGCAC GCTATTATGT GCACGACATG CCCAGTGCTT CGAGCTGTCT GGACAACAAC 
CACCAGGCCT TCCTCTTAGT GGATGATGGA AGTGTGGGTC ACAGAGGCAG TGAGACATCA 
TTCAGAGCCA ACCTGGAGGA CTTCATCTCC CATCAGCGCA CTGGCATCTG GGGAAGTGGA 
AGCATTGAGA TTCCTGTTTT GTGTGTGCTG ATTGCAGGAG ATGCTACAAT GCTCGAGAGA 
GTGGATTTAT CTCTGAAAAA CAGCACTCCA TGGCTGGTGC TGGCAGGGTC GGGGTCTGCA 
TCAGACCTGA TTGCAGGAAT ACTGAAGGAC CTCAGCGCTC CTCTGAGTCC ACCCGTGAGT 
CCCACTTTGC AAAGCGACTC TGAGGAAAGC AGCAAGAGCT CCAGCATTGA GCTCAGGGAC 
CGAGTGAAGG AGAGAATTAG GAAACACTTC CCTTCTGAGA CTGAACTGGA CAAGCTTGTG 
GATCAGGCTC TCAGCATCTA TCATAATAGA GATCTGATCA CTATCTTTCA TGGAGATGAG 
GATGGGTCAG ATGACTTTGA CACAATCATC CTGAAAGCTC TTGTAAGAGC TGCTAAACGT 
GATTCTAATG ATGCAAGCGA GTACACAGAA GAGCTGAAGC TGGCGGTGGC CTGGAACAGA 
GTGGATATTG CCAAGACTGA ACTCTTCAAT GGTGATGTCC ACTGGAGGTA TGAGGACCTG 
GAGGAATCGA TGACTGATGC TCTGGTAAAC GACAAGCCAG ATTTTGTGCG TCTGTTTGTG 
GAAAGCGGCT TAAACATTTT GGCCTATCTG ACGTACCATC GTTTGGAAAG TCTTTACAGC 
TCACTGTCGG ACTGTGATCT GGTGTCCTCA TTCCTCCAAA GGCGGCTGCA GGAGCGAGTG 
GGCTTAGCTG CCTCTCAGAC TCGATTAACT CCTCAACCTG ATGACAATTC CTCTTTCAAG 
AGCCAAGCCA CCAACCTTAC CACACCATCT GCTGCTCGAG ACCTCACTCT GTATGAGGTT 
TCACGAGTGT TATCAGAAAT TATGGAAGAT GTCTGCCAGC CATTTTACTA CACGACTCTT 
GGTTTAGATC ATAGAGTCAA CTCAAGGAAA GCAATGAAGC ATGTGAATAA GTTACTGCTA 
AATGAGAGTA CTTACCGACG GCAGCGTTGT ACATCCCCCT GGATATCTCT GTTCATTTGG 
GCTGTTCTGC AGAATCGCAG AGAAATGTCG CTATACTTCT GGAAAATGAC TGGAGAGTCT 
GTACTAAGTG CTCTTGCCGG GTGTAAGATT TTAAGAGAGC TGTCCAAACT GGAGACAGAG 
ACTGAGACTA AGCTCTCCAT GAAGGAACTT GCTCAAACCT TTGAAAATCT GGCACATGAT 
GTGTTCAGTG AGTGTTACCA GAGCAATGAG AAACGCTCCT TCAAGCTTCT CATCAGACAG 
TCTCCTATAT GGGGTGGTGC CACCTGTCTG CAGATGGCCA CATCGGCAGA CGCACGCCTC 
TTCTTCAGCC ATGATGGAGT TCAGACTTTA TTGTCAGAGA TTTGGTGGGG GGACATGGAC 
AGCAACACAG AGGTGTGGAA ACTGGTCTTC TCATTTTTCC TGCCTCCATT CATCTACACC 
AAGCTCATCA ATTTCAAGGT GGAGGAGGCC GATGAAAAAG CAGAGGACTT TGGTCAGGGT 
AAAGAATTTG ACAGCACTGA TGGAGCCGAG ACATTGGCTG ACATGACGAA CATGGATGAA 
GATGTGGAGG AATGCAATGC TTTAAGAGGA AAACCCAAAG CATCAAAGGT CATCGCTAAG 
AAACGGCCTT TCATATTCCA TCGCTGGCGG CAGTTCTGGT TTGCACCGGT CACTTCATTT 
CTAGGCAATG TGTTGATGTA CTTCCTCTTC CTCTTCCTGT TTGCTTACGT CTTGTTGATT 
GACTTCAAGC CTCCGCCTCC CGATGGACCT GCTCCTTTAG AGCTTGTGTT GTATTTCTGG 
GTTTTTACGC TGGCCTGTGA GGAAATTCGA CAGTCTTTCT TTGTAGGATC TACCACTTTA 
ACTCAAAGGA TGAAGGTGTA CATCCAGGAT ATCTGGAACA AGTGTGACAT CACAGCCCTC 
GGGCTCTTCG TTCTTGGGCT TCTCTGCAGG ATGTTTCCAT GGACATATAA TTTCGGTCGT 
GCAGTTATGT GTGTTGACTA TACAGTCTTC ACACTCAGAC TTATTCATAT ATTTGCAGTT 
CACAAACAAC TTGGTCCAAA AATCATTATA GTTGGCAAAA TGGTGAAAGA TGTGTTCTTC 
TTCCTGTTCT TCCTGGGGGT GTGGCTGATG GCATATGGAG TGGCCAATCA GGCTCTGCTG 
TACTCCTATG ACTCTCGCCC TGGTTGGGTA GCTCGGCGTG TGTTCTACAG GCCATATATG 
CACATATACG GGCAGATACC ACTGGATGAA ATTGATGCTG ATAAGCGACA CGACAAACGG 
TGTACGGATA ATGTCACTCT CATCCACGAG GGGGCAGAGC CCTGCCCAGA CTCTGATGCT 
AATTGGCTTG TCCTTATTCT ACTGTCAGTC TACTTGCTGG TCACCAATAT TCTGTTGGTG 
AACTTGCTCA TTGCTATGTT CAGTTACACA TTCAATAAAG TCCAGGAGCG CAGTGATGTC 
TACTGGAAGT TCCAGCGCTA CAACCTGATT GTGGAGTACC ACTCGCGCCC CTGCCTCGCC 
CCGCCCTTCA TCATCATCTC CCACCTGCAT ATCTTCATCA AGAGGGTCAT TCAGAGATTG 
CCTTCCCTTA AGAGCCAACA CTTCATGTTG GACCTGACAA GCAAAGAGAC GAGCTTGTTG 
AACACATGGG AATTGTCACT GAAAGAGAAC CTGCTGTCCA CGGAGAGCAA GAAGAAAAGA 
GAGAGCGACA CGGAGCGACT CAAACGCACC TCCACAAAGG TGGACAGCGT CTTGAAGCAG 
ATGGTGGAGA TTCGTGATCA CGATCGCAGG TTGAGAAAGC TGGAGTCTGA GGTGGAGTAC 
TGCTCTACTG CCCTCTCCTG GATAGTGGAG GCACTGTCCC AGAGTGATCT TGTGAAGCAG 
ACTCGTCCAC CTCCTTATCC TTCCAAAGGT ATTGCTTCAA AACGTCCCAC ATTTTCCAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001275744.2
CDS	95..3877
Translation	MIKMKRVEKESEGCGGGERKAQKDQSWIPKMIKKRVCTTFVEDSCSNGALCQCGGARDLHVSTTADDHVVGQWDRARDTSDYPTDAFGQLEFVGAGRRHCPFLRLSFDTPPDNVYSLMTSQWGLPVPNLVVSMVGGDEKEMAKPWVREVIRQGLVHAAQSTGAWIMTSGLKEGVSRCVGEAVRHHCAMASSLSQAKVIAVGVAPWGLIYQRHQLVNPQGSFPARYYVHDMPSASSCLDNNHQAFLLVDDGSVGHRGSETSFRANLEDFISHQRTGIWGSGSIEIPVLCVLIAGDATMLERVDLSLKNSTPWLVLAGSGSASDLIAGILKDLSAPLSPPVSPTLQSDSEESSKSSSIELRDRVKERIRKHFPSETELDKLVDQALSIYHNRDLITIFHGDEDGSDDFDTIILKALVRAAKRDSNDASEYTEELKLAVAWNRVDIAKTELFNGDVHWRYEDLEESMTDALVNDKPDFVRLFVESGLNILAYLTYHRLESLYSSLSDCDLVSSFLQRRLQERVGLAASQTRLTPQPDDNSSFKSQATNLTTPSAARDLTLYEVSRVLSEIMEDVCQPFYYTTLGLDHRVNSRKAMKHVNKLLLNESTYRRQRCTSPWISLFIWAVLQNRREMSLYFWKMTGESVLSALAGCKILRELSKLETETETKLSMKELAQTFENLAHDVFSECYQSNEKRSFKLLIRQSPIWGGATCLQMATSADARLFFSHDGVQTLLSEIWWGDMDSNTEVWKLVFSFFLPPFIYTKLINFKVEEADEKAEDFGQGKEFDSTDGAETLADMTNMDEDVEECNALRGKPKASKVIAKKRPFIFHRWRQFWFAPVTSFLGNVLMYFLFLFLFAYVLLIDFKPPPPDGPAPLELVLYFWVFTLACEEIRQSFFVGSTTLTQRMKVYIQDIWNKCDITALGLFVLGLLCRMFPWTYNFGRAVMCVDYTVFTLRLIHIFAVHKQLGPKIIIVGKMVKDVFFFLFFLGVWLMAYGVANQALLYSYDSRPGWVARRVFYRPYMHIYGQIPLDEIDADKRHDKRCTDNVTLIHEGAEPCPDSDANWLVLILLSVYLLVTNILLVNLLIAMFSYTFNKVQERSDVYWKFQRYNLIVEYHSRPCLAPPFIIISHLHIFIKRVIQRLPSLKSQHFMLDLTSKETSLLNTWELSLKENLLSTESKKKRESDTERLKRTSTKVDSVLKQMVEIRDHDRRLRKLESEVEYCSTALSWIVEALSQSDLVKQTRPPPYPSKGIASKRPTFSR

Target ORF information:

RefSeq Version	NM_001288815.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily M, member 4a (trpm4a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001288815.2

ORF Insert Sequence:

ATGATCAAAA TGAAGAGAGT CGAGAAAGAG AGTGAAGGCT GTGGGGGAGG AGAGAGAAAA 
GCCCAAAAGG ATCAGAGTTG GATCCCAAAG ATGATTAAAA AGAGAGTGTG CACCACCTTT 
GTGGAGGATT CATGCAGTAA TGGAGCTTTA TGTCAGTGTG GTGGAGCACG GGACCTTCAT 
GTATCTACAA CAGCAGATGA TCATGTAGTC GGCCAATGGG ACAGAGCACG GGACACCTCT 
GATTATCCCA CTGATGCCTT TGGACAGCTG GAGTTTGTTG GAGCCGGACG GAGACACTGT 
CCATTTCTGC GTCTGTCATT CGACACCCCT CCTGATAATG TGTACTCGCT GATGACGTCT 
CAGTGGGGTT TGCCGGTGCC CAATCTAGTG GTTTCCATGG TGGGTGGAGA TGAAAAAGAG 
ATGGCCAAAC CCTGGGTTCG AGAAGTGATC AGACAGGGTC TTGTCCATGC CGCTCAGAGC 
ACAGGGGCTT GGATCATGAC GTCAGGTCTG AAGGAGGGTG TTAGCCGTTG TGTAGGTGAG 
GCCGTCAGGC ATCATTGTGC AATGGCTTCA TCTCTTTCTC AGGCTAAAGT TATTGCCGTT 
GGTGTTGCAC CATGGGGGTT AATTTACCAA CGTCATCAAC TTGTAAACCC ACAGGGTAGT 
TTTCCTGCAC GCTATTATGT GCACGACATG CCCAGTGCTT CGAGCTGTCT GGACAACAAC 
CACCAGGCCT TCCTCTTAGT GGATGATGGA AGTGTGGGTC ACAGAGGCAG TGAGACATCA 
TTCAGAGCCA ACCTGGAGGA CTTCATCTCC CATCAGCGCA CTGGCATCTG GGGAAGTGGA 
AGCATTGAGA TTCCTGTTTT GTGTGTGCTG ATTGCAGGAG ATGCTACAAT GCTCGAGAGA 
GTGGATTTAT CTCTGAAAAA CAGCACTCCA TGGCTGGTGC TGGCAGGGTC GGGGTCTGCA 
TCAGACCTGA TTGCAGGAAT ACTGAAGGAC CTCAGCGCTC CTCTGAGTCC ACCCGTGAGT 
CCCACTTTGC AAAGCGACTC TGAGGAAAGC AGCAAGAGCT CCAGCATTGA GCTCAGGGAC 
CGAGTGAAGG AGAGAATTAG GAAACACTTC CCTTCTGAGA CTGAACTGGA CAAGCTTGTG 
GATCAGGCTC TCAGCATCTA TCATAATAGA GATCTGATCA CTATCTTTCA TGGAGATGAG 
GATGGGTCAG ATGACTTTGA CACAATCATC CTGAAAGCTC TTGTAAGAGC TGCTAAACGT 
GATTCTAATG ATGCAAGCGA GTACACAGAA GAGCTGAAGC TGGCGGTGGC CTGGAACAGA 
GTGGATATTG CCAAGACTGA ACTCTTCAAT GGTGATGTCC ACTGGAGGTA TGAGGACCTG 
GAGGAATCGA TGACTGATGC TCTGGTAAAC GACAAGCCAG ATTTTGTGCG TCTGTTTGTG 
GAAAGCGGCT TAAACATTTT GGCCTATCTG ACGTACCATC GTTTGGAAAG TCTTTACAGC 
TCACTGTCGG ACTGTGATCT GGTGTCCTCA TTCCTCCAAA GGCGGCTGCA GGAGCGAGTG 
GGCTTAGCTG CCTCTCAGAC TCGATTAACT CCTCAACCTG ATGACAATTC CTCTTTCAAG 
AGCCAAGCCA CCAACCTTAC CACACCATCT GCTGCTCGAG ACCTCACTCT GTATGAGGTT 
TCACGAGTGT TATCAGAAAT TATGGAAGAT GTCTGCCAGC CATTTTACTA CACGACTCTT 
GGTTTAGATC ATAGAGTCAA CTCAAGGAAA GCAATGAAGC ATGTGAATAA GTTACTGCTA 
AATGAGAGTA CTTACCGACG GCAGCGTTGT ACATCCCCCT GGATATCTCT GTTCATTTGG 
GCTGTTCTGC AGAATCGCAG AGAAATGTCG CTATACTTCT GGAAAATGAC TGGAGAGTCT 
GTACTAAGTG CTCTTGCCGG GTGTAAGATT TTAAGAGAGC TGTCCAAACT GGAGACAGAG 
ACTGAGACTA AGCTCTCCAT GAAGGAACTT GCTCAAACCT TTGAAAATCT GGCACATGAT 
GTGTTCAGTG AGTGTTACCA GAGCAATGAG AAACGCTCCT TCAAGCTTCT CATCAGACAG 
TCTCCTATAT GGGGTGGTGC CACCTGTCTG CAGATGGCCA CATCGGCAGA CGCACGCCTC 
TTCTTCAGCC ATGATGGAGT TCAGACTTTA TTGTCAGAGA TTTGGTGGGG GGACATGGAC 
AGCAACACAG AGGTGTGGAA ACTGGTCTTC TCATTTTTCC TGCCTCCATT CATCTACACC 
AAGCTCATCA ATTTCAAGGT GGAGGAGGCC GATGAAAAAG CAGAGGACTT TGGTCAGGGT 
AAAGAATTTG ACAGCACTGA TGGAGCCGAG ACATTGGCTG ACATGACGAA CATGGATGAA 
GATGTGGAGG AATGCAATGC TTTAAGAGGA AAACCCAAAG CATCAAAGGT CATCGCTAAG 
AAACGGCCTT TCATATTCCA TCGCTGGCGG CAGTTCTGGT TTGCACCGGT CACTTCATTT 
CTAGGCAATG TGTTGATGTA CTTCCTCTTC CTCTTCCTGT TTGCTTACGT CTTGTTGATT 
GACTTCAAGC CTCCGCCTCC CGATGGACCT GCTCCTTTAG AGCTTGTGTT GTATTTCTGG 
GTTTTTACGC TGGCCTGTGA GGAAATTCGA CAGTCTTTCT TTGTAGGATC TACCACTTTA 
ACTCAAAGGA TGAAGGTGTA CATCCAGGAT ATCTGGAACA AGTGTGACAT CACAGCCCTC 
GGGCTCTTCG TTCTTGGGCT TCTCTGCAGG ATGTTTCCAT GGACATATAA TTTCGGTCGT 
GCAGTTATGT GTGTTGACTA TACAGTCTTC ACACTCAGAC TTATTCATAT ATTTGCAGTT 
CACAAACAAC TTGGTCCAAA AATCATTATA GTTGGCAAAA TGGTGAAAGA TGTGTTCTTC 
TTCCTGTTCT TCCTGGGGGT GTGGCTGATG GCATATGGAG TGGCCAATCA GGCTCTGCTG 
TACTCCTATG ACTCTCGCCC TGGTTGGGTA GCTCGGCGTG TGTTCTACAG GCCATATATG 
CACATATACG GGCAGATACC ACTGGATGAA ATTGATGCTG ATAAGCGACA CGACAAACGG 
TGTACGGATA ATGTCACTCT CATCCACGAG GGGGCAGAGC CCTGCCCAGA CTCTGATGCT 
AATTGGCTTG TCCTTATTCT ACTGTCAGTC TACTTGCTGG TCACCAATAT TCTGTTGGTG 
AACTTGCTCA TTGCTATGTT CAGTTACACA TTCAATAAAG TCCAGGAGCG CAGTGATGTC 
TACTGGAAGT TCCAGCGCTA CAACCTGATT GTGGAGTACC ACTCGCGCCC CTGCCTCGCC 
CCGCCCTTCA TCATCATCTC CCACCTGCAT ATCTTCATCA AGAGGGTCAT TCAGAGATTG 
CCTTCCCTTA AGAGCCAACA CTTCATGTTG GACCTGACAA GCAAAGAGAC GAGCTTGTTG 
AACACATGGG AATTGTCACT GAAAGAGAAC CTGCTGTCCA CGGAGAGCAA GAAGAAAAGA 
GAGAGCGACA CGGAGCGACT CAAACGCACC TCCACAAAGG TGGACAGCGT CTTGAAGCAG 
ATGGTGGAGA TTCGTGATCA CGATCGCAGG TTGAGAAAGC TGGAGTCTGA GGTGGAGTAC 
TGCTCTACTG CCCTCTCCTG GATAGTGGAG GCACTGTCCC AGAGTGATCT TGTGAAGCAG 
ACTCGTCCAC CTCCTTATCC TTCCAAAGGT ATTGCTTCAA AACGTCCCAC ATTTTCCAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRPM4A ( XM_021468873.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021324548.1 Danio rerio transient receptor potential cation channel, subfamily M, member 4a (trpm4a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa47963
Clone ID Related Accession (Same CDS sequence)	XM_021468873.1
Accession Version	XM_021468873.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3096bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transient receptor potential cation channel, subfamily M, member 4a isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAGTG CTTCGAGCTG TCTGGACAAC AACCACCAGG CCTTCCTCTT AGTGGATGAT 
GGAAGTGTGG GTCACAGAGG CAGTGAGACA TCATTCAGAG CCAACCTGGA GGACTTCATC 
TCCCATCAGC GCACTGGCAT CTGGGGAAGT GGAAGCATTG AGATTCCTGT TTTGTGTGTG 
CTGATTGCAG GAGATGCTAC AATGCTCGAG AGAGTGGATT TATCTCTGAA AAACAGCACT 
CCATGGCTGG TGCTGGCAGG GTCGGGGTCT GCATCAGACC TGATTGCAGG AATACTGAAG 
GACCTCAGCG CTCCTCTGAG TCCACCCGTG AGTCCCACTT TGCAAAGCGA CTCTGAGGAA 
AGCAGCAAGA GCTCCAGCAT TGAGCTCAGG GACCGAGTGA AGGAGAGAAT TAGGAAACAC 
TTCCCTTCTG AGACTGAACT GGACAAGCTT GTGGATCAGG CTCTCAGCAT CTATCATAAT 
AGAGATCTGA TCACTATCTT TCATGGAGAT GAGGATGGGT CAGATGACTT TGACACAATC 
ATCCTGAAAG CTCTTGTAAG AGCTGCTAAA CGTGATTCTA ATGATGCAAG CGAGTACACA 
GAAGAGCTGA AGCTGGCGGT GGCCTGGAAC AGAGTGGATA TTGCCAAGAC TGAACTCTTC 
AATGGTGATG TCCACTGGAG GTATGAGGAC CTGGAGGAAT CGATGACTGA TGCTCTGGTA 
AACGACAAGC CAGATTTTGT GCGTCTGTTT GTGGAAAGCG GCTTAAACAT TTTGGCCTAT 
CTGACGTACC ATCGTTTGGA AAGTCTTTAC AGCTCACTGT CGGACTGTGA TCTGGTGTCC 
TCATTCCTCC AAAGGCGGCT GCAGGAGCGA GTGGGCTTAG CTGCCTCTCA GACTCGATTA 
ACTCCTCAAC CTGATGACAA TTCCTCTTTC AAGAGCCAAG CCACCAACCT TACCACACCA 
TCTGCTGCTC GAGACCTCAC TCTGTATGAG GTTTCACGAG TGTTATCAGA AATTATGGAA 
GATGTCTGCC AGCCATTTTA CTACACGACT CTTGGTTTAG ATCATAGAGT CAACTCAAGG 
AAAGCAATGA AGCATGTGAA TAAGTTACTG CTAAATGAGA GTACTTACCG ACGGCAGCGT 
TGTACATCCC CCTGGATATC TCTGTTCATT TGGGCTGTTC TGCAGAATCG CAGAGAAATG 
TCGCTATACT TCTGGAAAAT GACTGGAGAG TCTGTACTAA GTGCTCTTGC CGGGTGTAAG 
ATTTTAAGAG AGCTGTCCAA ACTGGAGACA GAGACTGAGA CTAAGCTCTC CATGAAGGAA 
CTTGCTCAAA CCTTTGAAAA TCTGGCACAT GATGTGTTCA GTGAGTGTTA CCAGAGCAAT 
GAGAAACGCT CCTTCAAGCT TCTCATCAGA CAGTCTCCTA TATGGGGTGG TGCCACCTGT 
CTGCAGATGG CCACATCGGC AGACGCACGC CTCTTCTTCA GCCATGATGG AGTTCAGACT 
TTATTGTCAG AGATTTGGTG GGGGGACATG GACAGCAACA CAGAGGTGTG GAAACTGGTC 
TTCTCATTTT TCCTGCCTCC ATTCATCTAC ACCAAGCTCA TCAATTTCAA GGTGGAGGAG 
GCCGATGAAA AAGCAGAGGA CTTTGGTCAG GGTAAAGAAT TTGACAGCAC TGATGGAGCC 
GAGACATTGG CTGACATGAC GAACATGGAT GAAGATGTGG AGGAATGCAA TGCTTTAAGA 
GGAAAACCCA AAGCATCAAA GGTCATCGCT AAGAAACGGC CTTTCATATT CCATCGCTGG 
CGGCAGTTCT GGTTTGCACC GGTCACTTCA TTTCTAGGCA ATGTGTTGAT GTACTTCCTC 
TTCCTCTTCC TGTTTGCTTA CGTCTTGTTG ATTGACTTCA AGCCTCCGCC TCCCGATGGA 
CCTGCTCCTT TAGAGCTTGT GTTGTATTTC TGGGTTTTTA CGCTGGCCTG TGAGGAAATT 
CGACAGTCTT TCTTTGTAGG ATCTACCACT TTAACTCAAA GGATGAAGGT GTACATCCAG 
GATATCTGGA ACAAGTGTGA CATCACAGCC CTCGGGCTCT TCGTTCTTGG GCTTCTCTGC 
AGGATGTTTC CATGGACATA TAATTTCGGT CGTGCAGTTA TGTGTGTTGA CTATACAGTC 
TTCACACTCA GACTTATTCA TATATTTGCA GTTCACAAAC AACTTGGTCC AAAAATCATT 
ATAGTTGGCA AAATGGTGAA AGATGTGTTC TTCTTCCTGT TCTTCCTGGG GGTGTGGCTG 
ATGGCATATG GAGTGGCCAA TCAGGCTCTG CTGTACTCCT ATGACTCTCG CCCTGGTTGG 
GTAGCTCGGC GTGTGTTCTA CAGGCCATAT ATGCACATAT ACGGGCAGAT ACCACTGGAT 
GAAATTGATG CTGATAAGCG ACACGACAAA CGGTGTACGG ATAATGTCAC TCTCATCCAC 
GAGGGGGCAG AGCCCTGCCC AGACTCTGAT GCTAATTGGC TTGTCCTTAT TCTACTGTCA 
GTCTACTTGC TGGTCACCAA TATTCTGTTG GTGAACTTGC TCATTGCTAT GTTCAGTTAC 
ACATTCAATA AAGTCCAGGA GCGCAGTGAT GTCTACTGGA AGTTCCAGCG CTACAACCTG 
ATTGTGGAGT ACCACTCGCG CCCCTGCCTC GCCCCGCCCT TCATCATCAT CTCCCACCTG 
CATATCTTCA TCAAGAGGGT CATTCAGAGA TTGCCTTCCC TTAAGAGCCA ACACTTCATG 
TTGGACCTGA CAAGCAAAGA GACGAGCTTG TTGAACACAT GGGAATTGTC ACTGAAAGAG 
AACCTGCTGT CCACGGAGAG CAAGAAGAAA AGAGAGAGCG ACACGGAGCG ACTCAAACGC 
ACCTCCACAA AGGTGGACAG CGTCTTGAAG CAGATGGTGG AGATTCGTGA TCACGATCGC 
AGGTTGAGAA AGCTGGAGTC TGAGGTGGAG TACTGCTCTA CTGCCCTCTC CTGGATAGTG 
GAGGCACTGT CCCAGAGTGA TCTTGTGAAG CAGACTCGTC CACCTCCTTA TCCTTCCAAA 
GGTATTGCTT CAAAACGTCC CACATTTTCC AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324548.1
CDS	153..3248
Translation	MPSASSCLDNNHQAFLLVDDGSVGHRGSETSFRANLEDFISHQRTGIWGSGSIEIPVLCVLIAGDATMLERVDLSLKNSTPWLVLAGSGSASDLIAGILKDLSAPLSPPVSPTLQSDSEESSKSSSIELRDRVKERIRKHFPSETELDKLVDQALSIYHNRDLITIFHGDEDGSDDFDTIILKALVRAAKRDSNDASEYTEELKLAVAWNRVDIAKTELFNGDVHWRYEDLEESMTDALVNDKPDFVRLFVESGLNILAYLTYHRLESLYSSLSDCDLVSSFLQRRLQERVGLAASQTRLTPQPDDNSSFKSQATNLTTPSAARDLTLYEVSRVLSEIMEDVCQPFYYTTLGLDHRVNSRKAMKHVNKLLLNESTYRRQRCTSPWISLFIWAVLQNRREMSLYFWKMTGESVLSALAGCKILRELSKLETETETKLSMKELAQTFENLAHDVFSECYQSNEKRSFKLLIRQSPIWGGATCLQMATSADARLFFSHDGVQTLLSEIWWGDMDSNTEVWKLVFSFFLPPFIYTKLINFKVEEADEKAEDFGQGKEFDSTDGAETLADMTNMDEDVEECNALRGKPKASKVIAKKRPFIFHRWRQFWFAPVTSFLGNVLMYFLFLFLFAYVLLIDFKPPPPDGPAPLELVLYFWVFTLACEEIRQSFFVGSTTLTQRMKVYIQDIWNKCDITALGLFVLGLLCRMFPWTYNFGRAVMCVDYTVFTLRLIHIFAVHKQLGPKIIIVGKMVKDVFFFLFFLGVWLMAYGVANQALLYSYDSRPGWVARRVFYRPYMHIYGQIPLDEIDADKRHDKRCTDNVTLIHEGAEPCPDSDANWLVLILLSVYLLVTNILLVNLLIAMFSYTFNKVQERSDVYWKFQRYNLIVEYHSRPCLAPPFIIISHLHIFIKRVIQRLPSLKSQHFMLDLTSKETSLLNTWELSLKENLLSTESKKKRESDTERLKRTSTKVDSVLKQMVEIRDHDRRLRKLESEVEYCSTALSWIVEALSQSDLVKQTRPPPYPSKGIASKRPTFSR

Target ORF information:

RefSeq Version	XM_021468873.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily M, member 4a (trpm4a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468873.1

ORF Insert Sequence:

ATGCCCAGTG CTTCGAGCTG TCTGGACAAC AACCACCAGG CCTTCCTCTT AGTGGATGAT 
GGAAGTGTGG GTCACAGAGG CAGTGAGACA TCATTCAGAG CCAACCTGGA GGACTTCATC 
TCCCATCAGC GCACTGGCAT CTGGGGAAGT GGAAGCATTG AGATTCCTGT TTTGTGTGTG 
CTGATTGCAG GAGATGCTAC AATGCTCGAG AGAGTGGATT TATCTCTGAA AAACAGCACT 
CCATGGCTGG TGCTGGCAGG GTCGGGGTCT GCATCAGACC TGATTGCAGG AATACTGAAG 
GACCTCAGCG CTCCTCTGAG TCCACCCGTG AGTCCCACTT TGCAAAGCGA CTCTGAGGAA 
AGCAGCAAGA GCTCCAGCAT TGAGCTCAGG GACCGAGTGA AGGAGAGAAT TAGGAAACAC 
TTCCCTTCTG AGACTGAACT GGACAAGCTT GTGGATCAGG CTCTCAGCAT CTATCATAAT 
AGAGATCTGA TCACTATCTT TCATGGAGAT GAGGATGGGT CAGATGACTT TGACACAATC 
ATCCTGAAAG CTCTTGTAAG AGCTGCTAAA CGTGATTCTA ATGATGCAAG CGAGTACACA 
GAAGAGCTGA AGCTGGCGGT GGCCTGGAAC AGAGTGGATA TTGCCAAGAC TGAACTCTTC 
AATGGTGATG TCCACTGGAG GTATGAGGAC CTGGAGGAAT CGATGACTGA TGCTCTGGTA 
AACGACAAGC CAGATTTTGT GCGTCTGTTT GTGGAAAGCG GCTTAAACAT TTTGGCCTAT 
CTGACGTACC ATCGTTTGGA AAGTCTTTAC AGCTCACTGT CGGACTGTGA TCTGGTGTCC 
TCATTCCTCC AAAGGCGGCT GCAGGAGCGA GTGGGCTTAG CTGCCTCTCA GACTCGATTA 
ACTCCTCAAC CTGATGACAA TTCCTCTTTC AAGAGCCAAG CCACCAACCT TACCACACCA 
TCTGCTGCTC GAGACCTCAC TCTGTATGAG GTTTCACGAG TGTTATCAGA AATTATGGAA 
GATGTCTGCC AGCCATTTTA CTACACGACT CTTGGTTTAG ATCATAGAGT CAACTCAAGG 
AAAGCAATGA AGCATGTGAA TAAGTTACTG CTAAATGAGA GTACTTACCG ACGGCAGCGT 
TGTACATCCC CCTGGATATC TCTGTTCATT TGGGCTGTTC TGCAGAATCG CAGAGAAATG 
TCGCTATACT TCTGGAAAAT GACTGGAGAG TCTGTACTAA GTGCTCTTGC CGGGTGTAAG 
ATTTTAAGAG AGCTGTCCAA ACTGGAGACA GAGACTGAGA CTAAGCTCTC CATGAAGGAA 
CTTGCTCAAA CCTTTGAAAA TCTGGCACAT GATGTGTTCA GTGAGTGTTA CCAGAGCAAT 
GAGAAACGCT CCTTCAAGCT TCTCATCAGA CAGTCTCCTA TATGGGGTGG TGCCACCTGT 
CTGCAGATGG CCACATCGGC AGACGCACGC CTCTTCTTCA GCCATGATGG AGTTCAGACT 
TTATTGTCAG AGATTTGGTG GGGGGACATG GACAGCAACA CAGAGGTGTG GAAACTGGTC 
TTCTCATTTT TCCTGCCTCC ATTCATCTAC ACCAAGCTCA TCAATTTCAA GGTGGAGGAG 
GCCGATGAAA AAGCAGAGGA CTTTGGTCAG GGTAAAGAAT TTGACAGCAC TGATGGAGCC 
GAGACATTGG CTGACATGAC GAACATGGAT GAAGATGTGG AGGAATGCAA TGCTTTAAGA 
GGAAAACCCA AAGCATCAAA GGTCATCGCT AAGAAACGGC CTTTCATATT CCATCGCTGG 
CGGCAGTTCT GGTTTGCACC GGTCACTTCA TTTCTAGGCA ATGTGTTGAT GTACTTCCTC 
TTCCTCTTCC TGTTTGCTTA CGTCTTGTTG ATTGACTTCA AGCCTCCGCC TCCCGATGGA 
CCTGCTCCTT TAGAGCTTGT GTTGTATTTC TGGGTTTTTA CGCTGGCCTG TGAGGAAATT 
CGACAGTCTT TCTTTGTAGG ATCTACCACT TTAACTCAAA GGATGAAGGT GTACATCCAG 
GATATCTGGA ACAAGTGTGA CATCACAGCC CTCGGGCTCT TCGTTCTTGG GCTTCTCTGC 
AGGATGTTTC CATGGACATA TAATTTCGGT CGTGCAGTTA TGTGTGTTGA CTATACAGTC 
TTCACACTCA GACTTATTCA TATATTTGCA GTTCACAAAC AACTTGGTCC AAAAATCATT 
ATAGTTGGCA AAATGGTGAA AGATGTGTTC TTCTTCCTGT TCTTCCTGGG GGTGTGGCTG 
ATGGCATATG GAGTGGCCAA TCAGGCTCTG CTGTACTCCT ATGACTCTCG CCCTGGTTGG 
GTAGCTCGGC GTGTGTTCTA CAGGCCATAT ATGCACATAT ACGGGCAGAT ACCACTGGAT 
GAAATTGATG CTGATAAGCG ACACGACAAA CGGTGTACGG ATAATGTCAC TCTCATCCAC 
GAGGGGGCAG AGCCCTGCCC AGACTCTGAT GCTAATTGGC TTGTCCTTAT TCTACTGTCA 
GTCTACTTGC TGGTCACCAA TATTCTGTTG GTGAACTTGC TCATTGCTAT GTTCAGTTAC 
ACATTCAATA AAGTCCAGGA GCGCAGTGAT GTCTACTGGA AGTTCCAGCG CTACAACCTG 
ATTGTGGAGT ACCACTCGCG CCCCTGCCTC GCCCCGCCCT TCATCATCAT CTCCCACCTG 
CATATCTTCA TCAAGAGGGT CATTCAGAGA TTGCCTTCCC TTAAGAGCCA ACACTTCATG 
TTGGACCTGA CAAGCAAAGA GACGAGCTTG TTGAACACAT GGGAATTGTC ACTGAAAGAG 
AACCTGCTGT CCACGGAGAG CAAGAAGAAA AGAGAGAGCG ACACGGAGCG ACTCAAACGC 
ACCTCCACAA AGGTGGACAG CGTCTTGAAG CAGATGGTGG AGATTCGTGA TCACGATCGC 
AGGTTGAGAA AGCTGGAGTC TGAGGTGGAG TACTGCTCTA CTGCCCTCTC CTGGATAGTG 
GAGGCACTGT CCCAGAGTGA TCTTGTGAAG CAGACTCGTC CACCTCCTTA TCCTTCCAAA 
GGTATTGCTT CAAAACGTCC CACATTTTCC AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRPM4A ( NM_001288815.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001275744.1 Danio rerio transient receptor potential cation channel, subfamily M, member 4a (trpm4a), mRNA.

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CloneID	ODa35835
Clone ID Related Accession (Same CDS sequence)	NM_001288815.1
Accession Version	NM_001288815.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-05
Organism	Danio rerio(zebrafish)
Product	transient receptor potential cation channel, subfamily M, member 4a
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from KF305309.1. On Jan 7, 2014 this sequence version replaced XM_003198133.2. ##Evidence-Data-START## Transcript exon combination :: KF305309.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGATCAAAA TGAAGAGAGT CGAGAAAGAG AGTGAAGGCT GTGGGGGAGG AGAGAGAAAA 
GCCCAAAAGG ATCAGAGTTG GATCCCAAAG ATGATTAAAA AGAGAGTGTG CACCACCTTT 
GTGGAGGATT CATGCAGTAA TGGAGCTTTA TGTCAGTGTG GTGGAGCACG GGACCTTCAT 
GTATCTACAA CAGCAGATGA TCATGTAGTC GGCCAATGGG ACAGAGCACG GGACACCTCT 
GATTATCCCA CTGATGCCTT TGGACAGCTG GAGTTTGTTG GAGCCGGACG GAGACACTGT 
CCATTTCTGC GTCTGTCATT CGACACCCCT CCTGATAATG TGTACTCGCT GATGACATCT 
CAGTGGGGCT TACCGGTGCC CAATCTAGTG GTTTCCATGG TGGGTGGAGA TGAAAAAGAG 
ATGGCCAAAC CCTGGGTTCG AGAAGTGATC AGACAGGGTC TTGTCCATGC CGCTCAGAGC 
ACAGGGGCTT GGATCATGAC GTCGGGTCTG AAGGAGGGTG TTAGCCGTTG TGTAGGTGAG 
GCCGTCAGGC ATCATTGTGC AATGGCTTCA TCTCTTTCTC AGGCTAAAGT TATTGCCGTT 
GGTGTTGCAC CATGGGGGTT AATTTACCAA CGTCATCAAC TTGTAAACCC ACAGGGTAGT 
TTTCCTGCAC GCTATTATGT CCACGACATG CCCAGTGCAT CGAGCTGTCT GGACAACAAC 
CACCAGGCCT TCCTCTTAGT GGATGATGGA AGTGTGGGTC ACAGAGGCAG TGAGACATCA 
TTCAGAGCCA ACCTGGAGGA CTTCATCTCC CATCAGCGCA CTGGCATCTG GGGAAGTGGA 
AGTATTGAGA TTCCTGTTTT GTGTGTGCTG ATTGCAGGAG ATGCTACAAT GCTCAAGAGA 
GTGGATTTAT CTCTGAAAAA CAGCACTCCA TGGCTGGTGC TGGCAGGGTC GGGGTCTGCA 
GCAGACCTGA TTGCAGGAAT ACTGAAGGAC CTCAGCGCTC CTCTGAGCCC ACCCGTGAGT 
CCCACTTTGC AAAGCGACTC TGAGGAAAGC AGCAAGAGCT CCAGCATTGA GCTCAGGGAC 
CGAGTGAAGG AGAGAATTAG GAAACACTTC CCTTCTGAGA CTGAACTGGA CAAGCTTGTG 
GATCAGGCTC TCAGCATCTA TCATAATAGA GATCTGATCA CTATCTTTCA TGGAGATGAG 
GATGGGTCAG ATGACTTTGA CACAATCATC CTGAAAGCTC TTGTAAGAGC TGCTAAACGT 
GATTCTAATG ATGCAAGCGA GTACACAGAA GAGCTGAAGC TGGCGGTGGC CTGGAACAGA 
GTGGATATTG CCAAGACTGA ACTCTTCAAT GGTGATGTCC ACTGGAGGTA TGAGGACCTG 
GAGGAATCGA TGACTGATGC TCTGGTAAAC GACAAGCCAG ATTTTGTGCG TCTGTTTGTG 
GAAAGCGGCT TAAACATTTT GGCCTATCTG ACGTACCATC GTTTGGAAAG TCTTTACAGC 
TCACTGTCGG ACTGTGATCT GGTTTCCTCA TTCCTCCAGA GGCGGCTGCA GGAGCGAGTG 
GGCTTAGCTG CCTCTCAGAC TCGATTAACT CCTCAACCTG ATGACAATTC CTCTTTCAAG 
AGCCGAGCCA CCAACCTTAC CACACCATCT GCTGCTCGAG ACCTCACTCT GTATGAGGTT 
TCACGAGTGT TATCAGAAAT TATGGAAGAT GTCTGCCAGC CATTTTACTA CACGACTCTT 
GGTTTAGATC ATAGAGTCAA CTCAAGGAAA GCAATGAAGC ATGTGAATAA GTTGCTGCTA 
AATGAGAGTA CTTACCGGCG GCAGCGTTGT ACATCCCCCT GGATATCTCT GTTCATTTGG 
GCTGTTCTGC AGAATCGCAG AGAAATGTCG CTATACTTCT GGAAAATGAC TGGAGAGTCT 
GTACTAAGTG CTCTTGCCGG GTGTAAGATT TTAAGAGAGC TGTCCAAACT GGAGACAGAG 
ACTGAGACTA AGCTCTCCAT GAAGGAACTT GCTCAAACCT TTGAAAATCT GGCACATGAT 
GTGTTCAGTG AGTGTTACCA GAGCAATGAG AAACGCTCCT TCAAGCTTCT CATCAGACAG 
TCTCCTATAT GGGGTGGTGC CACCTGTCTG CAGATGGCCA CATCGGCAGA CGCACGCCTC 
TTCTTCAGCC ATGATGGAGT TCAGACTTTA TTGTCAGAGA TTTGGTGGGG GGACATGGAC 
AGCAACACAG AGGTGTGGAA ACTGGTCTTC TCATTTTTCC TGCCTCCATT CATCTACACC 
AAGCTCATCA ATTTCAAGGT GGAGGAGACC GATGAAAAAG CAGAGGACTT TGGCCAGGGT 
AAAGAATTTG ACAGCACTGA TGGAGCCGAG ACGTTGGCTG ACATGACGAA CATGGATGAA 
GATGTGGAGG AATGCAATGC TTTAAGAGGA AAACCCAAAG CATCAAAGGT CATCGCTAAG 
AAACGGCCTT TCATATTCCA TCGCTGGCGG CAGTTCTGGT TTGCACCGGT CACTTCATTT 
CTAGGCAATG TGTTGATGTA CTTCCTCTTC CTCTTCCTGT TTGCTTACGT CTTGTTGATT 
GACTTCAAGC CTCCGCCTCC CGATGGACCT GCTTCTTTAG AGCTTGTGTT GTATTTCTGG 
GTTTTTACGC TGGCCTGTGA GGAAATTCGA CAGTCTTTCT TTGTAGGATC TACCACTTTA 
ACTCAAAGGA TGAAGGTGTA CATCCAGGAT ATCTGGAACA AGTGTGACAT CACAGCCCTC 
GGGCTCTTCG TTCTTGGGCT TCTCTGCAGG ATGTTTCCAT GGACATATAA TTTCGGTCGT 
GCAGTTATGT GTGTTGACTA TACAGTCTTC ACACTCAGAC TTATTCATAT ATTTGCAGTT 
CACAAACAAC TTGGTCCAAA AATCATTATA GTTGGCAAAA TGGTGAAAGA TGTGTTCTTC 
TTCCTGTTCT TCCTGGGGGT GTGGCTGATG GCATATGGAG TGGCCAATCA GGCTCTGCTG 
TACTCCTATG ACTCTCGCCC TGGTTGGGTA GCTCGGCGTG TGTTCTACAG GCCATATATG 
CACATATACG GGCAGATACC ACTGGATGAA ATTGATGCTG ATAAGCGACA CGACAAACGG 
TGTACGGATA ATGTCACTCT CATCCACGAG GGGGCAGAGC CCTGCCCAGA CTCTGATGCT 
AATTGGCTTG TCCTTATTCT ACTGTCAGTC TACTTGCTGG TCACCAATAT TCTGTTGGTG 
AACTTGCTCA TTGCTATGTT CAGTTACACA TTCAATAAAG TCCAGGAGCG CAGTGATGTC 
TACTGGAAGT TCCAGCGCTA CAACCTGATT GTGGAGTACC ACTCGCGCCC CTGCCTCGCC 
CCGCCCTTCA TCATCATCTC CCACCTGCAT ATCTTCATCA AGAGGGTCAT TCAGAGATTG 
CCTTCCCTTA AGAGCCAACA CTTCATGTTG GACCTGACAA GCAAAGAGAC GAGCTTGTTG 
AACACATGGG AATTGTCACT GAAAGAGAAC CTGCTGTCCA CGGAGAGCAA GAAGAAAAGA 
GAGAGCGACA CGGAGCGACT CAAACGCACC TCCACAAAGG TGGACAGCGT CTTAAAGCAG 
ATGGTGGAGA TTCGTGATCA CGATCGCAGG TTGAGAAAGC TGGAGTCTGA GGTGGAGTAC 
TGCTCTACTG CCCTCTCCTG GATAGTGGAG GCACTGTCCC AGAGTGATCT TGTGAAGCAG 
ACTCGTCCAC CTCCTTATCC TTCCAAAGGT ATTGCTTCAA AACGTCCCAC ATTTTCCAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001275744.1
CDS	39..3821
Translation	MIKMKRVEKESEGCGGGERKAQKDQSWIPKMIKKRVCTTFVEDSCSNGALCQCGGARDLHVSTTADDHVVGQWDRARDTSDYPTDAFGQLEFVGAGRRHCPFLRLSFDTPPDNVYSLMTSQWGLPVPNLVVSMVGGDEKEMAKPWVREVIRQGLVHAAQSTGAWIMTSGLKEGVSRCVGEAVRHHCAMASSLSQAKVIAVGVAPWGLIYQRHQLVNPQGSFPARYYVHDMPSASSCLDNNHQAFLLVDDGSVGHRGSETSFRANLEDFISHQRTGIWGSGSIEIPVLCVLIAGDATMLKRVDLSLKNSTPWLVLAGSGSAADLIAGILKDLSAPLSPPVSPTLQSDSEESSKSSSIELRDRVKERIRKHFPSETELDKLVDQALSIYHNRDLITIFHGDEDGSDDFDTIILKALVRAAKRDSNDASEYTEELKLAVAWNRVDIAKTELFNGDVHWRYEDLEESMTDALVNDKPDFVRLFVESGLNILAYLTYHRLESLYSSLSDCDLVSSFLQRRLQERVGLAASQTRLTPQPDDNSSFKSRATNLTTPSAARDLTLYEVSRVLSEIMEDVCQPFYYTTLGLDHRVNSRKAMKHVNKLLLNESTYRRQRCTSPWISLFIWAVLQNRREMSLYFWKMTGESVLSALAGCKILRELSKLETETETKLSMKELAQTFENLAHDVFSECYQSNEKRSFKLLIRQSPIWGGATCLQMATSADARLFFSHDGVQTLLSEIWWGDMDSNTEVWKLVFSFFLPPFIYTKLINFKVEETDEKAEDFGQGKEFDSTDGAETLADMTNMDEDVEECNALRGKPKASKVIAKKRPFIFHRWRQFWFAPVTSFLGNVLMYFLFLFLFAYVLLIDFKPPPPDGPASLELVLYFWVFTLACEEIRQSFFVGSTTLTQRMKVYIQDIWNKCDITALGLFVLGLLCRMFPWTYNFGRAVMCVDYTVFTLRLIHIFAVHKQLGPKIIIVGKMVKDVFFFLFFLGVWLMAYGVANQALLYSYDSRPGWVARRVFYRPYMHIYGQIPLDEIDADKRHDKRCTDNVTLIHEGAEPCPDSDANWLVLILLSVYLLVTNILLVNLLIAMFSYTFNKVQERSDVYWKFQRYNLIVEYHSRPCLAPPFIIISHLHIFIKRVIQRLPSLKSQHFMLDLTSKETSLLNTWELSLKENLLSTESKKKRESDTERLKRTSTKVDSVLKQMVEIRDHDRRLRKLESEVEYCSTALSWIVEALSQSDLVKQTRPPPYPSKGIASKRPTFSR

Target ORF information:

RefSeq Version	NM_001288815.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily M, member 4a (trpm4a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001288815.1

ORF Insert Sequence:

ATGATCAAAA TGAAGAGAGT CGAGAAAGAG AGTGAAGGCT GTGGGGGAGG AGAGAGAAAA 
GCCCAAAAGG ATCAGAGTTG GATCCCAAAG ATGATTAAAA AGAGAGTGTG CACCACCTTT 
GTGGAGGATT CATGCAGTAA TGGAGCTTTA TGTCAGTGTG GTGGAGCACG GGACCTTCAT 
GTATCTACAA CAGCAGATGA TCATGTAGTC GGCCAATGGG ACAGAGCACG GGACACCTCT 
GATTATCCCA CTGATGCCTT TGGACAGCTG GAGTTTGTTG GAGCCGGACG GAGACACTGT 
CCATTTCTGC GTCTGTCATT CGACACCCCT CCTGATAATG TGTACTCGCT GATGACATCT 
CAGTGGGGCT TACCGGTGCC CAATCTAGTG GTTTCCATGG TGGGTGGAGA TGAAAAAGAG 
ATGGCCAAAC CCTGGGTTCG AGAAGTGATC AGACAGGGTC TTGTCCATGC CGCTCAGAGC 
ACAGGGGCTT GGATCATGAC GTCGGGTCTG AAGGAGGGTG TTAGCCGTTG TGTAGGTGAG 
GCCGTCAGGC ATCATTGTGC AATGGCTTCA TCTCTTTCTC AGGCTAAAGT TATTGCCGTT 
GGTGTTGCAC CATGGGGGTT AATTTACCAA CGTCATCAAC TTGTAAACCC ACAGGGTAGT 
TTTCCTGCAC GCTATTATGT CCACGACATG CCCAGTGCAT CGAGCTGTCT GGACAACAAC 
CACCAGGCCT TCCTCTTAGT GGATGATGGA AGTGTGGGTC ACAGAGGCAG TGAGACATCA 
TTCAGAGCCA ACCTGGAGGA CTTCATCTCC CATCAGCGCA CTGGCATCTG GGGAAGTGGA 
AGTATTGAGA TTCCTGTTTT GTGTGTGCTG ATTGCAGGAG ATGCTACAAT GCTCAAGAGA 
GTGGATTTAT CTCTGAAAAA CAGCACTCCA TGGCTGGTGC TGGCAGGGTC GGGGTCTGCA 
GCAGACCTGA TTGCAGGAAT ACTGAAGGAC CTCAGCGCTC CTCTGAGCCC ACCCGTGAGT 
CCCACTTTGC AAAGCGACTC TGAGGAAAGC AGCAAGAGCT CCAGCATTGA GCTCAGGGAC 
CGAGTGAAGG AGAGAATTAG GAAACACTTC CCTTCTGAGA CTGAACTGGA CAAGCTTGTG 
GATCAGGCTC TCAGCATCTA TCATAATAGA GATCTGATCA CTATCTTTCA TGGAGATGAG 
GATGGGTCAG ATGACTTTGA CACAATCATC CTGAAAGCTC TTGTAAGAGC TGCTAAACGT 
GATTCTAATG ATGCAAGCGA GTACACAGAA GAGCTGAAGC TGGCGGTGGC CTGGAACAGA 
GTGGATATTG CCAAGACTGA ACTCTTCAAT GGTGATGTCC ACTGGAGGTA TGAGGACCTG 
GAGGAATCGA TGACTGATGC TCTGGTAAAC GACAAGCCAG ATTTTGTGCG TCTGTTTGTG 
GAAAGCGGCT TAAACATTTT GGCCTATCTG ACGTACCATC GTTTGGAAAG TCTTTACAGC 
TCACTGTCGG ACTGTGATCT GGTTTCCTCA TTCCTCCAGA GGCGGCTGCA GGAGCGAGTG 
GGCTTAGCTG CCTCTCAGAC TCGATTAACT CCTCAACCTG ATGACAATTC CTCTTTCAAG 
AGCCGAGCCA CCAACCTTAC CACACCATCT GCTGCTCGAG ACCTCACTCT GTATGAGGTT 
TCACGAGTGT TATCAGAAAT TATGGAAGAT GTCTGCCAGC CATTTTACTA CACGACTCTT 
GGTTTAGATC ATAGAGTCAA CTCAAGGAAA GCAATGAAGC ATGTGAATAA GTTGCTGCTA 
AATGAGAGTA CTTACCGGCG GCAGCGTTGT ACATCCCCCT GGATATCTCT GTTCATTTGG 
GCTGTTCTGC AGAATCGCAG AGAAATGTCG CTATACTTCT GGAAAATGAC TGGAGAGTCT 
GTACTAAGTG CTCTTGCCGG GTGTAAGATT TTAAGAGAGC TGTCCAAACT GGAGACAGAG 
ACTGAGACTA AGCTCTCCAT GAAGGAACTT GCTCAAACCT TTGAAAATCT GGCACATGAT 
GTGTTCAGTG AGTGTTACCA GAGCAATGAG AAACGCTCCT TCAAGCTTCT CATCAGACAG 
TCTCCTATAT GGGGTGGTGC CACCTGTCTG CAGATGGCCA CATCGGCAGA CGCACGCCTC 
TTCTTCAGCC ATGATGGAGT TCAGACTTTA TTGTCAGAGA TTTGGTGGGG GGACATGGAC 
AGCAACACAG AGGTGTGGAA ACTGGTCTTC TCATTTTTCC TGCCTCCATT CATCTACACC 
AAGCTCATCA ATTTCAAGGT GGAGGAGACC GATGAAAAAG CAGAGGACTT TGGCCAGGGT 
AAAGAATTTG ACAGCACTGA TGGAGCCGAG ACGTTGGCTG ACATGACGAA CATGGATGAA 
GATGTGGAGG AATGCAATGC TTTAAGAGGA AAACCCAAAG CATCAAAGGT CATCGCTAAG 
AAACGGCCTT TCATATTCCA TCGCTGGCGG CAGTTCTGGT TTGCACCGGT CACTTCATTT 
CTAGGCAATG TGTTGATGTA CTTCCTCTTC CTCTTCCTGT TTGCTTACGT CTTGTTGATT 
GACTTCAAGC CTCCGCCTCC CGATGGACCT GCTTCTTTAG AGCTTGTGTT GTATTTCTGG 
GTTTTTACGC TGGCCTGTGA GGAAATTCGA CAGTCTTTCT TTGTAGGATC TACCACTTTA 
ACTCAAAGGA TGAAGGTGTA CATCCAGGAT ATCTGGAACA AGTGTGACAT CACAGCCCTC 
GGGCTCTTCG TTCTTGGGCT TCTCTGCAGG ATGTTTCCAT GGACATATAA TTTCGGTCGT 
GCAGTTATGT GTGTTGACTA TACAGTCTTC ACACTCAGAC TTATTCATAT ATTTGCAGTT 
CACAAACAAC TTGGTCCAAA AATCATTATA GTTGGCAAAA TGGTGAAAGA TGTGTTCTTC 
TTCCTGTTCT TCCTGGGGGT GTGGCTGATG GCATATGGAG TGGCCAATCA GGCTCTGCTG 
TACTCCTATG ACTCTCGCCC TGGTTGGGTA GCTCGGCGTG TGTTCTACAG GCCATATATG 
CACATATACG GGCAGATACC ACTGGATGAA ATTGATGCTG ATAAGCGACA CGACAAACGG 
TGTACGGATA ATGTCACTCT CATCCACGAG GGGGCAGAGC CCTGCCCAGA CTCTGATGCT 
AATTGGCTTG TCCTTATTCT ACTGTCAGTC TACTTGCTGG TCACCAATAT TCTGTTGGTG 
AACTTGCTCA TTGCTATGTT CAGTTACACA TTCAATAAAG TCCAGGAGCG CAGTGATGTC 
TACTGGAAGT TCCAGCGCTA CAACCTGATT GTGGAGTACC ACTCGCGCCC CTGCCTCGCC 
CCGCCCTTCA TCATCATCTC CCACCTGCAT ATCTTCATCA AGAGGGTCAT TCAGAGATTG 
CCTTCCCTTA AGAGCCAACA CTTCATGTTG GACCTGACAA GCAAAGAGAC GAGCTTGTTG 
AACACATGGG AATTGTCACT GAAAGAGAAC CTGCTGTCCA CGGAGAGCAA GAAGAAAAGA 
GAGAGCGACA CGGAGCGACT CAAACGCACC TCCACAAAGG TGGACAGCGT CTTAAAGCAG 
ATGGTGGAGA TTCGTGATCA CGATCGCAGG TTGAGAAAGC TGGAGTCTGA GGTGGAGTAC 
TGCTCTACTG CCCTCTCCTG GATAGTGGAG GCACTGTCCC AGAGTGATCT TGTGAAGCAG 
ACTCGTCCAC CTCCTTATCC TTCCAAAGGT ATTGCTTCAA AACGTCCCAC ATTTTCCAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Thermal stress in Danio rerio: a link between temperature, light, thermo-TRP channels, and clock genes.
Journal of thermal biology68(Pt A)128-138(2017 Aug)
Jer?nimo R,Moraes MN,de Assis LVM,Ramos BC,Rocha T,Castrucci AML

Transcriptomic, proteomic, and metabolomic landscape of positional memory in the caudal fin of zebrafish.
Proceedings of the National Academy of Sciences of the United States of America114(5)E717-E726(2017 Jan)
Rabinowitz JS,Robitaille AM,Wang Y,Ray CA,Thummel R,Gu H,Djukovic D,Raftery D,Berndt JD,Moon RT

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Expression and functionality of transient receptor potential melastatin 4 (TRPM4)-like channels during development of the zebrafish.
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP17833-44(2015 Dec)
Cheng H,Ellis J,Kleinow KM

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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TRPM4A cDNA ORF clone, Danio rerio(Zebrafish)

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gRNAs

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ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

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