march6 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	march6
Entrez Gene ID	100002072
Full Name	membrane-associated ring finger (C3HC4) 6
Synonyms	fa98a02,im:7161807,si:dkey-40c17.2,wu:fa98a02
General protein information	Preferred Names membrane-associated ring finger (C3HC4) 6 Names E3 ubiquitin-protein ligase MARCH6
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005163449.3	XP_005163506.1	E3 ubiquitin-protein ligase MARCH6 isoform X1
XM_003197869.4	XP_003197917.1	E3 ubiquitin-protein ligase MARCH6 isoform X2
XM_005163449.4	XP_005163506.1	E3 ubiquitin-protein ligase MARCH6 isoform X1
XM_005163450.3	XP_005163507.1	E3 ubiquitin-protein ligase MARCH6 isoform X3
NM_001291707.1	NP_001278636.1	E3 ubiquitin-protein ligase MARCH6
XM_003197869.5	XP_003197917.1	E3 ubiquitin-protein ligase MARCH6 isoform X2
XM_005163450.4	XP_005163507.1	E3 ubiquitin-protein ligase MARCH6 isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MARCH6	XP_003310676.1
Canis lupus familiaris (dog)	MARCH6	XP_005639724.1
Gallus gallus (chicken)	MARCH6	XP_419012.3
Arabidopsis thaliana (thale cress)	AT4G34100	NP_195136.3
Mus musculus (house mouse)	March6	NP_766194.2
Drosophila melanogaster (fruit fly)	CG1317	NP_647715.2
Xenopus tropicalis (tropical clawed frog)	march6	XP_002938364.2
Homo sapiens (human)	MARCH6	NP_005876.2
Bos taurus (cattle)	MARCH6	NP_001192741.1
Rattus norvegicus (Norway rat)	March6	XP_215517.5
Danio rerio (zebrafish)	march6	XP_005163506.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007204	XP_308581.3
Caenorhabditis elegans (roundworm)	marc-6	NP_492823.2
Oryza sativa (rice)	Os06g0639000	NP_001058157.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium.
Gaudet P, Livstone MS, Lewis SE, Thomas PD
Briefings in bioinformatics12(5)449-62(2011 Sep)

Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Strausberg RL, Feingold EA, Grouse LH, Derge JG, Klausner RD, Collins FS, Wagner L, Shenmen CM, Schuler GD, Altschul SF, Zeeberg B, Buetow KH, Schaefer CF, Bhat NK, Hopkins RF, Jordan H, Moore T, Max SI, Wang J, Hsieh F, Diatchenko L, Marusina K, Farmer AA, Rubin GM, Hong L, Stapleton M, Soares MB, Bonaldo MF, Casavant TL, Scheetz TE, Brownstein MJ, Usdin TB, Toshiyuki S, Carninci P, Prange C, Raha SS, Loquellano NA, Peters GJ, Abramson RD, Mullahy SJ, Bosak SA, McEwan PJ, McKernan KJ, Malek JA, Gunaratne PH, Richards S, Worley KC, Hale S, Garcia AM, Gay LJ, Hulyk SW, Villalon DK, Muzny DM, Sodergren EJ, Lu X, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madan A, Young AC, Shevchenko Y, Bouffard GG, Blakesley RW, Touchman JW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Krzywinski MI, Skalska U, Smailus DE, Schnerch A, Schein JE, Jones SJ, Marra MA,
Proceedings of the National Academy of Sciences of the United States of America99(26)16899-903(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following march6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the march6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa01256	NM_001291707.1 Latest version!	Danio rerio membrane-associated ring finger (C3HC4) 6 (march6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa07100	XM_005163449.4 Latest version!	Danio rerio membrane-associated ring finger (C3HC4) 6 (march6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa07101	XM_003197869.5 Latest version!	Danio rerio membrane-associated ring finger (C3HC4) 6 (march6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa07102	XM_005163450.4 Latest version!	Danio rerio membrane-associated ring finger (C3HC4) 6 (march6), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
View Old Accession Versions >>
ODa07100	XM_005163449.3	Danio rerio membrane-associated ring finger (C3HC4) 6 (march6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa07101	XM_003197869.4	Danio rerio membrane-associated ring finger (C3HC4) 6 (march6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa07102	XM_005163450.3	Danio rerio membrane-associated ring finger (C3HC4) 6 (march6), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

march6 ( NM_001291707.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001278636.1 Danio rerio membrane-associated ring finger (C3HC4) 6 (march6), mRNA.

Price & Availability↑

CloneID	ODa01256
Clone ID Related Accession (Same CDS sequence)	NM_001291707.1
Accession Version	NM_001291707.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase MARCH6
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EB935699.1, CK024583.1, EB949406.1, EB899125.1, CT661348.1, CR388419.13 and EH451889.1. On Apr 25, 2014 this sequence version replaced XM_002660829.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## CDS exon combination :: GDQH01005419.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGACACTG CCGAAGAAGC TGATATATGT AGGGTGTGTC GATCTGAGGG AACCCAAGAC 
AAACCCCTCT ACCATCCCTG TGTGTGTACT GGAAGTATTA AGTTCATCCA CCAAGAATGC 
TTGGTGCAGT GGCTTAAACA CAGCAGAAAA GAATACTGCG AATTATGCAA GCACAGATTT 
GCTTTCACGC CAATCTACTC TCCAGACATG CCTTCACGGC TCCCAGTGCA GGACATTTTC 
GCAGGACTGG TCACCAGTAT AGGCACAGCA ATCCGATACT GGTTTCATTA CACGCTTGTT 
GCCTTTGCTT GGCTGGGGGT TGTACCATTA ACAGCATGTC GCATCTATAA GTGTCTGTTC 
ACTGGATCTG TAAGCTCGCT CCTGACTCTG CCATTAGACA TGCTCTCCAC AGAGAACTTG 
TTGGCCGACT GCCTGCAGGG CTGCTTTGTG GTGACCTGCA CCCTCTGCGC CTTCATCAGT 
CTGGTGTGGC TGCGGGAGCA GATTGTTCAC GGTGGAGCCC CTTTATGGTT GGAGCAGAAC 
CAGCAGCAAC CTGCCAATGC CGCAGGACCG CCCAATGAGG CCCCTGGCCA GGGTAATGGT 
GGTGCTGAGA ACCAGCCCAT GCCAGCTCCG GCTGAACCTC CAGCAGAAAA CGCTGCAGCT 
GCTGAGGCTC CTGACCTCCC CGCTGATCCT GCTGAAGAGA TGGAGCTTGA TAATGAGGAT 
GAGGATGATG GAGGAGCTGA AGATGTGGCA GATGCTAACA ATGGAGCACA AGATGATATG 
AACTGGAATG CTCTGGAATG GGACCGTGCA GCTGAAGAGC TCACTTGGGA AAGGATGCTT 
GGGCTTGATG GGTCTCTGGT TTTCCTGGAG CATGTGTTCT GGGTGGTCTC ACTCAACACT 
CTCTTCATTT TGGTGTTTGC TTTCTGCCCC TACCACATTG GCCACTTCTC AGTGGTTGGA 
CTTGGTTTTG AAGAATATGT TCGTGCTTCA CACTTTGAGG GGCTCATCAC CACTATTGTC 
GGATATGTCC TCTTAGCCAT CACACTCATT GTGTGTCATG GATTAGCAGC GCTGGTGAGA 
TTTCAAAGAT CACGCCGCTT GTTAGGCGTT TGCTACATAG TTGTAAAGGT GTCTCTTCTA 
GTAGTCGTAG AGATTGGTGT GTTCCCCCTC ATCTGCGGCT GGTGGCTTGA TATCTGCTCT 
TTGGAAATGT TTGATGCTTC ATTAAAAGAC AGAGAGTTGA GTTTCGAGTC AGCACCAGGT 
ACCACTATGT TTCTTCATTG GCTGGTGGGG ATGGTCTACG TCTTCTATTT TGCATCTTTT 
ATTCTTCTAC TTCGAGAGGT ACTGAGGCCT GGAGTATTAT GGTTTCTCAG AAATCTGAAT 
GATCCAGATT TCAACCCTGT GCAGGAAATG ATTCACCTGC CAATATACAG ACACCTGAGA 
CGATTCATAT TATCTGTGGT TGTGTTTGGC TCAATTGTTC TTCTCATGTT GTGGCTTCCT 
ATCCGGATAA TCAAGCACAT CTTTCCTTCA TTTCTACCCT ACAATGTGAT GCTTTACAGT 
GACGCTCCAG TGAGTGAGTT GTCTCTGGAG CTGCTGTTGT TGCAGGTCGT TCTTCCTGCA 
CTGTTGGAAC AAGGACACAC ACGGCAGTGG CTCAAAGGTC TAGTGCGAGC CTGGACCGTG 
ACCGCTGGAT ACTTGCTTGA TTTGCACTCG TACTTGCTGG GAGACCAGGA AGACAACGAG 
AACAATGCCA ACCAGCAAGC CAACAACAAT AACCAACAGG CCCGTAACAA TGCTATTCCT 
GTGGTGGGAG AAGGGCTGCA CGCTGCCCAC CAGGCTATAC TGCAGCAGGG CGGCCCGGTA 
GGATTCCAAC CCTATCATCG GCCTATGAAA TTTCCTCTAA GGATTGTCTT GCTGATATTG 
TTCATGTGTG TGACGCTACT TTTGGCCAGT TTAGTGTGTC TTACGTTACC AGTATTTACA 
GGACGCTGGC TGATGTCCTT CTGGACGGGC AGTGCTAAGA TCCACGAGCT GTACACTGCA 
GCATGTGGCC TCTATGTGTG CTGGCTCTCC ATCAGAGCGA TCACCGTACT GCTGGCCTGG 
ATGCCTCAGG GTCGCAGAGT CATCCTGCTC AAGATTCAGG AGTGGACCCT CATGATTATG 
AAGACCCTGA TTGTGGCTGT GCTGTTGGCT GGAGTGATTC CTCTGCTCTT GGGACTGCTG 
TTTGAGCTGG TGATTGTAGC TCCTCTCAGA GTGCCGCTGG ATCAGACTCC TTTGTTCTAC 
CCCTGGCAGG ACTGGGCTTT AGGAGTGCTT CATGCCAAAA TCATTGCTGC CATCACCCTC 
ATGGGTCCCC AGTGGTGGCT GAAGACCGTT ATTGAGCAGG TGTATGCCAA TGGAATCCGG 
AACATTGACC TTCATTTCAT CATCAGGAAG CTAGCGGCTC CGGTCATTGC TGTATTGCTG 
CTGTCTCTCT GCATCCCTTA TGTGATTGCT GTGGGCATCG TGCCCCTGCT TGGGGTCACG 
ATGGAGATGC AGAATCTTGT GCAGAGGAGA ATCTACCCCT TTCTCCTTAT GGTGGTGTTT 
CTGATGGGGA TCCTCTCTTT TCAAATCCGA CAATTCAAGC GCCTTTATGA GCACATCAAG 
AATGACAAGT ACCTGGTTGG ACAGAGACTA GTGAACTATG AACGGAAAGC TGGCAAAGCC 
AGTGCCAGCA CACACAGCAG TTCAGTTCAG GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001278636.1
CDS	213..2948
Translation	MDTAEEADICRVCRSEGTQDKPLYHPCVCTGSIKFIHQECLVQWLKHSRKEYCELCKHRFAFTPIYSPDMPSRLPVQDIFAGLVTSIGTAIRYWFHYTLVAFAWLGVVPLTACRIYKCLFTGSVSSLLTLPLDMLSTENLLADCLQGCFVVTCTLCAFISLVWLREQIVHGGAPLWLEQNQQQPANAAGPPNEAPGQGNGGAENQPMPAPAEPPAENAAAAEAPDLPADPAEEMELDNEDEDDGGAEDVADANNGAQDDMNWNALEWDRAAEELTWERMLGLDGSLVFLEHVFWVVSLNTLFILVFAFCPYHIGHFSVVGLGFEEYVRASHFEGLITTIVGYVLLAITLIVCHGLAALVRFQRSRRLLGVCYIVVKVSLLVVVEIGVFPLICGWWLDICSLEMFDASLKDRELSFESAPGTTMFLHWLVGMVYVFYFASFILLLREVLRPGVLWFLRNLNDPDFNPVQEMIHLPIYRHLRRFILSVVVFGSIVLLMLWLPIRIIKHIFPSFLPYNVMLYSDAPVSELSLELLLLQVVLPALLEQGHTRQWLKGLVRAWTVTAGYLLDLHSYLLGDQEDNENNANQQANNNNQQARNNAIPVVGEGLHAAHQAILQQGGPVGFQPYHRPMKFPLRIVLLILFMCVTLLLASLVCLTLPVFTGRWLMSFWTGSAKIHELYTAACGLYVCWLSIRAITVLLAWMPQGRRVILLKIQEWTLMIMKTLIVAVLLAGVIPLLLGLLFELVIVAPLRVPLDQTPLFYPWQDWALGVLHAKIIAAITLMGPQWWLKTVIEQVYANGIRNIDLHFIIRKLAAPVIAVLLLSLCIPYVIAVGIVPLLGVTMEMQNLVQRRIYPFLLMVVFLMGILSFQIRQFKRLYEHIKNDKYLVGQRLVNYERKAGKASASTHSSSVQE

Target ORF information:

RefSeq Version	NM_001291707.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio membrane-associated ring finger (C3HC4) 6 (march6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001291707.1

ORF Insert Sequence:

ATGGACACTG CCGAAGAAGC TGATATATGT AGGGTGTGTC GATCTGAGGG AACCCAAGAC 
AAACCCCTCT ACCATCCCTG TGTGTGTACT GGAAGTATTA AGTTCATCCA CCAAGAATGC 
TTGGTGCAGT GGCTTAAACA CAGCAGAAAA GAATACTGCG AATTATGCAA GCACAGATTT 
GCTTTCACGC CAATCTACTC TCCAGACATG CCTTCACGGC TCCCAGTGCA GGACATTTTC 
GCAGGACTGG TCACCAGTAT AGGCACAGCA ATCCGATACT GGTTTCATTA CACGCTTGTT 
GCCTTTGCTT GGCTGGGGGT TGTACCATTA ACAGCATGTC GCATCTATAA GTGTCTGTTC 
ACTGGATCTG TAAGCTCGCT CCTGACTCTG CCATTAGACA TGCTCTCCAC AGAGAACTTG 
TTGGCCGACT GCCTGCAGGG CTGCTTTGTG GTGACCTGCA CCCTCTGCGC CTTCATCAGT 
CTGGTGTGGC TGCGGGAGCA GATTGTTCAC GGTGGAGCCC CTTTATGGTT GGAGCAGAAC 
CAGCAGCAAC CTGCCAATGC CGCAGGACCG CCCAATGAGG CCCCTGGCCA GGGTAATGGT 
GGTGCTGAGA ACCAGCCCAT GCCAGCTCCG GCTGAACCTC CAGCAGAAAA CGCTGCAGCT 
GCTGAGGCTC CTGACCTCCC CGCTGATCCT GCTGAAGAGA TGGAGCTTGA TAATGAGGAT 
GAGGATGATG GAGGAGCTGA AGATGTGGCA GATGCTAACA ATGGAGCACA AGATGATATG 
AACTGGAATG CTCTGGAATG GGACCGTGCA GCTGAAGAGC TCACTTGGGA AAGGATGCTT 
GGGCTTGATG GGTCTCTGGT TTTCCTGGAG CATGTGTTCT GGGTGGTCTC ACTCAACACT 
CTCTTCATTT TGGTGTTTGC TTTCTGCCCC TACCACATTG GCCACTTCTC AGTGGTTGGA 
CTTGGTTTTG AAGAATATGT TCGTGCTTCA CACTTTGAGG GGCTCATCAC CACTATTGTC 
GGATATGTCC TCTTAGCCAT CACACTCATT GTGTGTCATG GATTAGCAGC GCTGGTGAGA 
TTTCAAAGAT CACGCCGCTT GTTAGGCGTT TGCTACATAG TTGTAAAGGT GTCTCTTCTA 
GTAGTCGTAG AGATTGGTGT GTTCCCCCTC ATCTGCGGCT GGTGGCTTGA TATCTGCTCT 
TTGGAAATGT TTGATGCTTC ATTAAAAGAC AGAGAGTTGA GTTTCGAGTC AGCACCAGGT 
ACCACTATGT TTCTTCATTG GCTGGTGGGG ATGGTCTACG TCTTCTATTT TGCATCTTTT 
ATTCTTCTAC TTCGAGAGGT ACTGAGGCCT GGAGTATTAT GGTTTCTCAG AAATCTGAAT 
GATCCAGATT TCAACCCTGT GCAGGAAATG ATTCACCTGC CAATATACAG ACACCTGAGA 
CGATTCATAT TATCTGTGGT TGTGTTTGGC TCAATTGTTC TTCTCATGTT GTGGCTTCCT 
ATCCGGATAA TCAAGCACAT CTTTCCTTCA TTTCTACCCT ACAATGTGAT GCTTTACAGT 
GACGCTCCAG TGAGTGAGTT GTCTCTGGAG CTGCTGTTGT TGCAGGTCGT TCTTCCTGCA 
CTGTTGGAAC AAGGACACAC ACGGCAGTGG CTCAAAGGTC TAGTGCGAGC CTGGACCGTG 
ACCGCTGGAT ACTTGCTTGA TTTGCACTCG TACTTGCTGG GAGACCAGGA AGACAACGAG 
AACAATGCCA ACCAGCAAGC CAACAACAAT AACCAACAGG CCCGTAACAA TGCTATTCCT 
GTGGTGGGAG AAGGGCTGCA CGCTGCCCAC CAGGCTATAC TGCAGCAGGG CGGCCCGGTA 
GGATTCCAAC CCTATCATCG GCCTATGAAA TTTCCTCTAA GGATTGTCTT GCTGATATTG 
TTCATGTGTG TGACGCTACT TTTGGCCAGT TTAGTGTGTC TTACGTTACC AGTATTTACA 
GGACGCTGGC TGATGTCCTT CTGGACGGGC AGTGCTAAGA TCCACGAGCT GTACACTGCA 
GCATGTGGCC TCTATGTGTG CTGGCTCTCC ATCAGAGCGA TCACCGTACT GCTGGCCTGG 
ATGCCTCAGG GTCGCAGAGT CATCCTGCTC AAGATTCAGG AGTGGACCCT CATGATTATG 
AAGACCCTGA TTGTGGCTGT GCTGTTGGCT GGAGTGATTC CTCTGCTCTT GGGACTGCTG 
TTTGAGCTGG TGATTGTAGC TCCTCTCAGA GTGCCGCTGG ATCAGACTCC TTTGTTCTAC 
CCCTGGCAGG ACTGGGCTTT AGGAGTGCTT CATGCCAAAA TCATTGCTGC CATCACCCTC 
ATGGGTCCCC AGTGGTGGCT GAAGACCGTT ATTGAGCAGG TGTATGCCAA TGGAATCCGG 
AACATTGACC TTCATTTCAT CATCAGGAAG CTAGCGGCTC CGGTCATTGC TGTATTGCTG 
CTGTCTCTCT GCATCCCTTA TGTGATTGCT GTGGGCATCG TGCCCCTGCT TGGGGTCACG 
ATGGAGATGC AGAATCTTGT GCAGAGGAGA ATCTACCCCT TTCTCCTTAT GGTGGTGTTT 
CTGATGGGGA TCCTCTCTTT TCAAATCCGA CAATTCAAGC GCCTTTATGA GCACATCAAG 
AATGACAAGT ACCTGGTTGG ACAGAGACTA GTGAACTATG AACGGAAAGC TGGCAAAGCC 
AGTGCCAGCA CACACAGCAG TTCAGTTCAG GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

march6 ( XM_005163449.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005163506.1 Danio rerio membrane-associated ring finger (C3HC4) 6 (march6), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa07100
Clone ID Related Accession (Same CDS sequence)	XM_005163449.3 , XM_005163449.4
Accession Version	XM_005163449.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2811bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase MARCH6 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005163449.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACTG CCGAAGAAGC TGATATATGT AGGGTGTGTC GATCTGAGGG AACCCAAGAC 
AAACCCCTCT ACCATCCCTG TGTGTGTACT GGAAGTATTA AGTTCATCCA CCAAGAATGC 
TTGGTGCAGT GGCTTAAACA CAGCAGAAAA GAATACTGCG AATTATGCAA GCACAGATTT 
GCTTTCACGC CAATCTACTC TCCAGACATG CCTTCACGGC TCCCAGTGCA GGACATTTTC 
GCAGGACTGG TCACCAGTAT AGGCACAGCA ATCCGATACT GGTTTCATTA CACGCTTGTT 
GCCTTTGCTT GGCTGGGGGT TGTACCATTA ACAGCATGTC GCATCTATAA GTGTCTGTTC 
ACTGGATCTG TAAGCTCGCT CCTGACTCTG CCATTAGACA TGCTCTCCAC GTACGTACCA 
GACAGGACAG CTCTCAGAGA GAACTTGTTG GCCGACTGCC TGCAGGGCTG CTTTGTGGTG 
ACCTGCACCC TCTGCGCCTT CATCAGTCTG GTGTGGCTGC GGGAGCAGAT TGTTCACGGT 
GGAGCCCCTT TATGGTTGGA GCAGAACCAG CAGCAACCTG CCAATGCCGC AGGACCGCCC 
AATGAGGCCC CTGGCCAGGG TAATGGTGGT GCTGAGAACC AGCCCATGCC AGCTCCGGCT 
GAACCTCCAG CAGAAAACGC TGCAGCTGCT GAGGCTCCTG ACCTCCCCGC TGATCCTGCT 
GAAGAGATGG AGCTTGATAA TGAGGATGAG GATGATGGAG GAGCTGAAGA TGTGGCAGAT 
GCTAACAATG GAGCACAAGA TGATATGAAC TGGAATGCTC TGGAATGGGA CCGTGCAGCT 
GAAGAGCTCA CTTGGGAAAG GATGCTTGGG CTTGATGGGT CTCTGGTTTT CCTGGAGCAT 
GTGTTCTGGG TGGTCTCACT CAACACTCTC TTCATTTTGG TGTTTGCTTT CTGCCCCTAC 
CACATTGGCC ACTTCTCAGT GGTTGGACTT GGTTTTGAAG AATATGTTCG TGCTTCACAC 
TTTGAGGGGC TCATCACCAC TATTGTCGGA TATGTCCTCT TAGCCATCAC ACTCATTGTG 
TGTCATGGAT TAGCAGCGCT GGTGAGATTT CAAAGATCAC GCCGCTTGTT AGGCGTTTGC 
TACATAGTTG TAAAGGTGTC TCTTCTAGTA GTCGTAGAGA TTGGTGTGTT CCCCCTCATC 
TGCGGCTGGT GGCTTGATAT CTGCTCTTTG GAAATGTTTG ATGCTTCATT AAAAGACAGA 
GAGTTGAGTT TCGAGTCAGC ACCAGGTACC ACTATGTTTC TTCATTGGCT GGTGGGGATG 
GTCTACGTCT TCTATTTTGC ATCTTTTATT CTTCTACTTC GAGAGGTACT GAGGCCTGGA 
GTATTATGGT TTCTCAGAAA TCTGAATGAT CCAGATTTCA ACCCTGTGCA GGAAATGATT 
CACCTGCCAA TATACAGACA CCTGAGACGA TTCATATTAT CTGTGGTTGT GTTTGGCTCA 
ATTGTTCTTC TCATGTTGTG GCTTCCTATC CGGATAATCA AGCACATCTT TCCTTCATTT 
CTACCCTACA ATGTGATGCT TTACAGTGAC GCTCCAGTGA GTGAGTTGTC TCTGGAGCTG 
CTGTTGTTGC AGGTCGTTCT TCCTGCACTG TTGGAACAAG GACACACACG GCAGTGGCTC 
AAAGGTCTAG TGCGAGCCTG GACCGTGACC GCTGGATACT TGCTTGATTT GCACTCGTAC 
TTGCTGGGAG ACCAGGAAGA CAACGAGAAC AATGCCAACC AGCAAGCCAA CAACAATAAC 
CAACAGGCCC GTAACAATGC TATTCCTGTG GTGGGAGAAG GGCTGCACGC TGCCCACCAG 
GCTATACTGC AGCAGGGCGG CCCGGTAGGA TTCCAACCCT ATCATCGGCC TATGAAATTT 
CCTCTAAGGA TTGTCTTGCT GATATTGTTC ATGTGTGTGA CGCTACTTTT GGCCAGTTTA 
GTGTGTCTTA CGTTACCAGT ATTTACAGGA CGCTGGCTGA TGTCCTTCTG GACGGGCAGT 
GCTAAGATCC ACGAGCTGTA CACTGCAGCA TGTGGCCTCT ATGTGTGCTG GCTCTCCATC 
AGAGCGATCA CCGTACTGCT GGCCTGGATG CCTCAGGGTC GCAGAGTCAT CCTGCTCAAG 
ATTCAGGAGT GGACCCTCAT GCTTCAAGAC AGCTCGTGTT TGAAGAATGG AGTCACATGC 
ATTGCTCAGA TTATGAAGAC CCTGATTGTG GCTGTGCTGT TGGCTGGAGT GATTCCTCTG 
CTCTTGGGAC TGCTGTTTGA GCTGGTGATT GTAGCTCCTC TCAGAGTGCC GCTGGATCAG 
ACTCCTTTGT TCTACCCCTG GCAGGACTGG GCTTTAGGAG TGCTTCATGC CAAAATCATT 
GCTGCCATCA CCCTCATGGG TCCCCAGTGG TGGCTGAAGA CCGTTATTGA GCAGGTGTAT 
GCCAATGGAA TCCGGAACAT TGACCTTCAT TTCATCATCA GGAAGCTAGC GGCTCCGGTC 
ATTGCTGTAT TGCTGCTGTC TCTCTGCATC CCTTATGTGA TTGCTGTGGG CATCGTGCCC 
CTGCTTGGGG TCACGATGGA GATGCAGAAT CTTGTGCAGA GGAGAATCTA CCCCTTTCTC 
CTTATGGTGG TGTTTCTGAT GGGGATCCTC TCTTTTCAAA TCCGACAATT CAAGCGCCTT 
TATGAGCACA TCAAGAATGA CAAGTACCTG GTTGGACAGA GACTAGTGAA CTATGAACGG 
AAAGCTGGCA AAGCCAGTGC CAGCACACAC AGCAGTTCAG TTCAGGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163506.1
CDS	277..3087
Translation	MDTAEEADICRVCRSEGTQDKPLYHPCVCTGSIKFIHQECLVQWLKHSRKEYCELCKHRFAFTPIYSPDMPSRLPVQDIFAGLVTSIGTAIRYWFHYTLVAFAWLGVVPLTACRIYKCLFTGSVSSLLTLPLDMLSTYVPDRTALRENLLADCLQGCFVVTCTLCAFISLVWLREQIVHGGAPLWLEQNQQQPANAAGPPNEAPGQGNGGAENQPMPAPAEPPAENAAAAEAPDLPADPAEEMELDNEDEDDGGAEDVADANNGAQDDMNWNALEWDRAAEELTWERMLGLDGSLVFLEHVFWVVSLNTLFILVFAFCPYHIGHFSVVGLGFEEYVRASHFEGLITTIVGYVLLAITLIVCHGLAALVRFQRSRRLLGVCYIVVKVSLLVVVEIGVFPLICGWWLDICSLEMFDASLKDRELSFESAPGTTMFLHWLVGMVYVFYFASFILLLREVLRPGVLWFLRNLNDPDFNPVQEMIHLPIYRHLRRFILSVVVFGSIVLLMLWLPIRIIKHIFPSFLPYNVMLYSDAPVSELSLELLLLQVVLPALLEQGHTRQWLKGLVRAWTVTAGYLLDLHSYLLGDQEDNENNANQQANNNNQQARNNAIPVVGEGLHAAHQAILQQGGPVGFQPYHRPMKFPLRIVLLILFMCVTLLLASLVCLTLPVFTGRWLMSFWTGSAKIHELYTAACGLYVCWLSIRAITVLLAWMPQGRRVILLKIQEWTLMLQDSSCLKNGVTCIAQIMKTLIVAVLLAGVIPLLLGLLFELVIVAPLRVPLDQTPLFYPWQDWALGVLHAKIIAAITLMGPQWWLKTVIEQVYANGIRNIDLHFIIRKLAAPVIAVLLLSLCIPYVIAVGIVPLLGVTMEMQNLVQRRIYPFLLMVVFLMGILSFQIRQFKRLYEHIKNDKYLVGQRLVNYERKAGKASASTHSSSVQE

Target ORF information:

RefSeq Version	XM_005163449.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio membrane-associated ring finger (C3HC4) 6 (march6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163449.3

ORF Insert Sequence:

ATGGACACTG CCGAAGAAGC TGATATATGT AGGGTGTGTC GATCTGAGGG AACCCAAGAC 
AAACCCCTCT ACCATCCCTG TGTGTGTACT GGAAGTATTA AGTTCATCCA CCAAGAATGC 
TTGGTGCAGT GGCTTAAACA CAGCAGAAAA GAATACTGCG AATTATGCAA GCACAGATTT 
GCTTTCACGC CAATCTACTC TCCAGACATG CCTTCACGGC TCCCAGTGCA GGACATTTTC 
GCAGGACTGG TCACCAGTAT AGGCACAGCA ATCCGATACT GGTTTCATTA CACGCTTGTT 
GCCTTTGCTT GGCTGGGGGT TGTACCATTA ACAGCATGTC GCATCTATAA GTGTCTGTTC 
ACTGGATCTG TAAGCTCGCT CCTGACTCTG CCATTAGACA TGCTCTCCAC GTACGTACCA 
GACAGGACAG CTCTCAGAGA GAACTTGTTG GCCGACTGCC TGCAGGGCTG CTTTGTGGTG 
ACCTGCACCC TCTGCGCCTT CATCAGTCTG GTGTGGCTGC GGGAGCAGAT TGTTCACGGT 
GGAGCCCCTT TATGGTTGGA GCAGAACCAG CAGCAACCTG CCAATGCCGC AGGACCGCCC 
AATGAGGCCC CTGGCCAGGG TAATGGTGGT GCTGAGAACC AGCCCATGCC AGCTCCGGCT 
GAACCTCCAG CAGAAAACGC TGCAGCTGCT GAGGCTCCTG ACCTCCCCGC TGATCCTGCT 
GAAGAGATGG AGCTTGATAA TGAGGATGAG GATGATGGAG GAGCTGAAGA TGTGGCAGAT 
GCTAACAATG GAGCACAAGA TGATATGAAC TGGAATGCTC TGGAATGGGA CCGTGCAGCT 
GAAGAGCTCA CTTGGGAAAG GATGCTTGGG CTTGATGGGT CTCTGGTTTT CCTGGAGCAT 
GTGTTCTGGG TGGTCTCACT CAACACTCTC TTCATTTTGG TGTTTGCTTT CTGCCCCTAC 
CACATTGGCC ACTTCTCAGT GGTTGGACTT GGTTTTGAAG AATATGTTCG TGCTTCACAC 
TTTGAGGGGC TCATCACCAC TATTGTCGGA TATGTCCTCT TAGCCATCAC ACTCATTGTG 
TGTCATGGAT TAGCAGCGCT GGTGAGATTT CAAAGATCAC GCCGCTTGTT AGGCGTTTGC 
TACATAGTTG TAAAGGTGTC TCTTCTAGTA GTCGTAGAGA TTGGTGTGTT CCCCCTCATC 
TGCGGCTGGT GGCTTGATAT CTGCTCTTTG GAAATGTTTG ATGCTTCATT AAAAGACAGA 
GAGTTGAGTT TCGAGTCAGC ACCAGGTACC ACTATGTTTC TTCATTGGCT GGTGGGGATG 
GTCTACGTCT TCTATTTTGC ATCTTTTATT CTTCTACTTC GAGAGGTACT GAGGCCTGGA 
GTATTATGGT TTCTCAGAAA TCTGAATGAT CCAGATTTCA ACCCTGTGCA GGAAATGATT 
CACCTGCCAA TATACAGACA CCTGAGACGA TTCATATTAT CTGTGGTTGT GTTTGGCTCA 
ATTGTTCTTC TCATGTTGTG GCTTCCTATC CGGATAATCA AGCACATCTT TCCTTCATTT 
CTACCCTACA ATGTGATGCT TTACAGTGAC GCTCCAGTGA GTGAGTTGTC TCTGGAGCTG 
CTGTTGTTGC AGGTCGTTCT TCCTGCACTG TTGGAACAAG GACACACACG GCAGTGGCTC 
AAAGGTCTAG TGCGAGCCTG GACCGTGACC GCTGGATACT TGCTTGATTT GCACTCGTAC 
TTGCTGGGAG ACCAGGAAGA CAACGAGAAC AATGCCAACC AGCAAGCCAA CAACAATAAC 
CAACAGGCCC GTAACAATGC TATTCCTGTG GTGGGAGAAG GGCTGCACGC TGCCCACCAG 
GCTATACTGC AGCAGGGCGG CCCGGTAGGA TTCCAACCCT ATCATCGGCC TATGAAATTT 
CCTCTAAGGA TTGTCTTGCT GATATTGTTC ATGTGTGTGA CGCTACTTTT GGCCAGTTTA 
GTGTGTCTTA CGTTACCAGT ATTTACAGGA CGCTGGCTGA TGTCCTTCTG GACGGGCAGT 
GCTAAGATCC ACGAGCTGTA CACTGCAGCA TGTGGCCTCT ATGTGTGCTG GCTCTCCATC 
AGAGCGATCA CCGTACTGCT GGCCTGGATG CCTCAGGGTC GCAGAGTCAT CCTGCTCAAG 
ATTCAGGAGT GGACCCTCAT GCTTCAAGAC AGCTCGTGTT TGAAGAATGG AGTCACATGC 
ATTGCTCAGA TTATGAAGAC CCTGATTGTG GCTGTGCTGT TGGCTGGAGT GATTCCTCTG 
CTCTTGGGAC TGCTGTTTGA GCTGGTGATT GTAGCTCCTC TCAGAGTGCC GCTGGATCAG 
ACTCCTTTGT TCTACCCCTG GCAGGACTGG GCTTTAGGAG TGCTTCATGC CAAAATCATT 
GCTGCCATCA CCCTCATGGG TCCCCAGTGG TGGCTGAAGA CCGTTATTGA GCAGGTGTAT 
GCCAATGGAA TCCGGAACAT TGACCTTCAT TTCATCATCA GGAAGCTAGC GGCTCCGGTC 
ATTGCTGTAT TGCTGCTGTC TCTCTGCATC CCTTATGTGA TTGCTGTGGG CATCGTGCCC 
CTGCTTGGGG TCACGATGGA GATGCAGAAT CTTGTGCAGA GGAGAATCTA CCCCTTTCTC 
CTTATGGTGG TGTTTCTGAT GGGGATCCTC TCTTTTCAAA TCCGACAATT CAAGCGCCTT 
TATGAGCACA TCAAGAATGA CAAGTACCTG GTTGGACAGA GACTAGTGAA CTATGAACGG 
AAAGCTGGCA AAGCCAGTGC CAGCACACAC AGCAGTTCAG TTCAGGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

march6 ( XM_005163449.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005163506.1 Danio rerio membrane-associated ring finger (C3HC4) 6 (march6), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa07100
Clone ID Related Accession (Same CDS sequence)	XM_005163449.3 , XM_005163449.4
Accession Version	XM_005163449.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2811bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase MARCH6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005163449.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACTG CCGAAGAAGC TGATATATGT AGGGTGTGTC GATCTGAGGG AACCCAAGAC 
AAACCCCTCT ACCATCCCTG TGTGTGTACT GGAAGTATTA AGTTCATCCA CCAAGAATGC 
TTGGTGCAGT GGCTTAAACA CAGCAGAAAA GAATACTGCG AATTATGCAA GCACAGATTT 
GCTTTCACGC CAATCTACTC TCCAGACATG CCTTCACGGC TCCCAGTGCA GGACATTTTC 
GCAGGACTGG TCACCAGTAT AGGCACAGCA ATCCGATACT GGTTTCATTA CACGCTTGTT 
GCCTTTGCTT GGCTGGGGGT TGTACCATTA ACAGCATGTC GCATCTATAA GTGTCTGTTC 
ACTGGATCTG TAAGCTCGCT CCTGACTCTG CCATTAGACA TGCTCTCCAC GTACGTACCA 
GACAGGACAG CTCTCAGAGA GAACTTGTTG GCCGACTGCC TGCAGGGCTG CTTTGTGGTG 
ACCTGCACCC TCTGCGCCTT CATCAGTCTG GTGTGGCTGC GGGAGCAGAT TGTTCACGGT 
GGAGCCCCTT TATGGTTGGA GCAGAACCAG CAGCAACCTG CCAATGCCGC AGGACCGCCC 
AATGAGGCCC CTGGCCAGGG TAATGGTGGT GCTGAGAACC AGCCCATGCC AGCTCCGGCT 
GAACCTCCAG CAGAAAACGC TGCAGCTGCT GAGGCTCCTG ACCTCCCCGC TGATCCTGCT 
GAAGAGATGG AGCTTGATAA TGAGGATGAG GATGATGGAG GAGCTGAAGA TGTGGCAGAT 
GCTAACAATG GAGCACAAGA TGATATGAAC TGGAATGCTC TGGAATGGGA CCGTGCAGCT 
GAAGAGCTCA CTTGGGAAAG GATGCTTGGG CTTGATGGGT CTCTGGTTTT CCTGGAGCAT 
GTGTTCTGGG TGGTCTCACT CAACACTCTC TTCATTTTGG TGTTTGCTTT CTGCCCCTAC 
CACATTGGCC ACTTCTCAGT GGTTGGACTT GGTTTTGAAG AATATGTTCG TGCTTCACAC 
TTTGAGGGGC TCATCACCAC TATTGTCGGA TATGTCCTCT TAGCCATCAC ACTCATTGTG 
TGTCATGGAT TAGCAGCGCT GGTGAGATTT CAAAGATCAC GCCGCTTGTT AGGCGTTTGC 
TACATAGTTG TAAAGGTGTC TCTTCTAGTA GTCGTAGAGA TTGGTGTGTT CCCCCTCATC 
TGCGGCTGGT GGCTTGATAT CTGCTCTTTG GAAATGTTTG ATGCTTCATT AAAAGACAGA 
GAGTTGAGTT TCGAGTCAGC ACCAGGTACC ACTATGTTTC TTCATTGGCT GGTGGGGATG 
GTCTACGTCT TCTATTTTGC ATCTTTTATT CTTCTACTTC GAGAGGTACT GAGGCCTGGA 
GTATTATGGT TTCTCAGAAA TCTGAATGAT CCAGATTTCA ACCCTGTGCA GGAAATGATT 
CACCTGCCAA TATACAGACA CCTGAGACGA TTCATATTAT CTGTGGTTGT GTTTGGCTCA 
ATTGTTCTTC TCATGTTGTG GCTTCCTATC CGGATAATCA AGCACATCTT TCCTTCATTT 
CTACCCTACA ATGTGATGCT TTACAGTGAC GCTCCAGTGA GTGAGTTGTC TCTGGAGCTG 
CTGTTGTTGC AGGTCGTTCT TCCTGCACTG TTGGAACAAG GACACACACG GCAGTGGCTC 
AAAGGTCTAG TGCGAGCCTG GACCGTGACC GCTGGATACT TGCTTGATTT GCACTCGTAC 
TTGCTGGGAG ACCAGGAAGA CAACGAGAAC AATGCCAACC AGCAAGCCAA CAACAATAAC 
CAACAGGCCC GTAACAATGC TATTCCTGTG GTGGGAGAAG GGCTGCACGC TGCCCACCAG 
GCTATACTGC AGCAGGGCGG CCCGGTAGGA TTCCAACCCT ATCATCGGCC TATGAAATTT 
CCTCTAAGGA TTGTCTTGCT GATATTGTTC ATGTGTGTGA CGCTACTTTT GGCCAGTTTA 
GTGTGTCTTA CGTTACCAGT ATTTACAGGA CGCTGGCTGA TGTCCTTCTG GACGGGCAGT 
GCTAAGATCC ACGAGCTGTA CACTGCAGCA TGTGGCCTCT ATGTGTGCTG GCTCTCCATC 
AGAGCGATCA CCGTACTGCT GGCCTGGATG CCTCAGGGTC GCAGAGTCAT CCTGCTCAAG 
ATTCAGGAGT GGACCCTCAT GCTTCAAGAC AGCTCGTGTT TGAAGAATGG AGTCACATGC 
ATTGCTCAGA TTATGAAGAC CCTGATTGTG GCTGTGCTGT TGGCTGGAGT GATTCCTCTG 
CTCTTGGGAC TGCTGTTTGA GCTGGTGATT GTAGCTCCTC TCAGAGTGCC GCTGGATCAG 
ACTCCTTTGT TCTACCCCTG GCAGGACTGG GCTTTAGGAG TGCTTCATGC CAAAATCATT 
GCTGCCATCA CCCTCATGGG TCCCCAGTGG TGGCTGAAGA CCGTTATTGA GCAGGTGTAT 
GCCAATGGAA TCCGGAACAT TGACCTTCAT TTCATCATCA GGAAGCTAGC GGCTCCGGTC 
ATTGCTGTAT TGCTGCTGTC TCTCTGCATC CCTTATGTGA TTGCTGTGGG CATCGTGCCC 
CTGCTTGGGG TCACGATGGA GATGCAGAAT CTTGTGCAGA GGAGAATCTA CCCCTTTCTC 
CTTATGGTGG TGTTTCTGAT GGGGATCCTC TCTTTTCAAA TCCGACAATT CAAGCGCCTT 
TATGAGCACA TCAAGAATGA CAAGTACCTG GTTGGACAGA GACTAGTGAA CTATGAACGG 
AAAGCTGGCA AAGCCAGTGC CAGCACACAC AGCAGTTCAG TTCAGGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163506.1
CDS	272..3082
Translation	MDTAEEADICRVCRSEGTQDKPLYHPCVCTGSIKFIHQECLVQWLKHSRKEYCELCKHRFAFTPIYSPDMPSRLPVQDIFAGLVTSIGTAIRYWFHYTLVAFAWLGVVPLTACRIYKCLFTGSVSSLLTLPLDMLSTYVPDRTALRENLLADCLQGCFVVTCTLCAFISLVWLREQIVHGGAPLWLEQNQQQPANAAGPPNEAPGQGNGGAENQPMPAPAEPPAENAAAAEAPDLPADPAEEMELDNEDEDDGGAEDVADANNGAQDDMNWNALEWDRAAEELTWERMLGLDGSLVFLEHVFWVVSLNTLFILVFAFCPYHIGHFSVVGLGFEEYVRASHFEGLITTIVGYVLLAITLIVCHGLAALVRFQRSRRLLGVCYIVVKVSLLVVVEIGVFPLICGWWLDICSLEMFDASLKDRELSFESAPGTTMFLHWLVGMVYVFYFASFILLLREVLRPGVLWFLRNLNDPDFNPVQEMIHLPIYRHLRRFILSVVVFGSIVLLMLWLPIRIIKHIFPSFLPYNVMLYSDAPVSELSLELLLLQVVLPALLEQGHTRQWLKGLVRAWTVTAGYLLDLHSYLLGDQEDNENNANQQANNNNQQARNNAIPVVGEGLHAAHQAILQQGGPVGFQPYHRPMKFPLRIVLLILFMCVTLLLASLVCLTLPVFTGRWLMSFWTGSAKIHELYTAACGLYVCWLSIRAITVLLAWMPQGRRVILLKIQEWTLMLQDSSCLKNGVTCIAQIMKTLIVAVLLAGVIPLLLGLLFELVIVAPLRVPLDQTPLFYPWQDWALGVLHAKIIAAITLMGPQWWLKTVIEQVYANGIRNIDLHFIIRKLAAPVIAVLLLSLCIPYVIAVGIVPLLGVTMEMQNLVQRRIYPFLLMVVFLMGILSFQIRQFKRLYEHIKNDKYLVGQRLVNYERKAGKASASTHSSSVQE

Target ORF information:

RefSeq Version	XM_005163449.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio membrane-associated ring finger (C3HC4) 6 (march6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163449.4

ORF Insert Sequence:

ATGGACACTG CCGAAGAAGC TGATATATGT AGGGTGTGTC GATCTGAGGG AACCCAAGAC 
AAACCCCTCT ACCATCCCTG TGTGTGTACT GGAAGTATTA AGTTCATCCA CCAAGAATGC 
TTGGTGCAGT GGCTTAAACA CAGCAGAAAA GAATACTGCG AATTATGCAA GCACAGATTT 
GCTTTCACGC CAATCTACTC TCCAGACATG CCTTCACGGC TCCCAGTGCA GGACATTTTC 
GCAGGACTGG TCACCAGTAT AGGCACAGCA ATCCGATACT GGTTTCATTA CACGCTTGTT 
GCCTTTGCTT GGCTGGGGGT TGTACCATTA ACAGCATGTC GCATCTATAA GTGTCTGTTC 
ACTGGATCTG TAAGCTCGCT CCTGACTCTG CCATTAGACA TGCTCTCCAC GTACGTACCA 
GACAGGACAG CTCTCAGAGA GAACTTGTTG GCCGACTGCC TGCAGGGCTG CTTTGTGGTG 
ACCTGCACCC TCTGCGCCTT CATCAGTCTG GTGTGGCTGC GGGAGCAGAT TGTTCACGGT 
GGAGCCCCTT TATGGTTGGA GCAGAACCAG CAGCAACCTG CCAATGCCGC AGGACCGCCC 
AATGAGGCCC CTGGCCAGGG TAATGGTGGT GCTGAGAACC AGCCCATGCC AGCTCCGGCT 
GAACCTCCAG CAGAAAACGC TGCAGCTGCT GAGGCTCCTG ACCTCCCCGC TGATCCTGCT 
GAAGAGATGG AGCTTGATAA TGAGGATGAG GATGATGGAG GAGCTGAAGA TGTGGCAGAT 
GCTAACAATG GAGCACAAGA TGATATGAAC TGGAATGCTC TGGAATGGGA CCGTGCAGCT 
GAAGAGCTCA CTTGGGAAAG GATGCTTGGG CTTGATGGGT CTCTGGTTTT CCTGGAGCAT 
GTGTTCTGGG TGGTCTCACT CAACACTCTC TTCATTTTGG TGTTTGCTTT CTGCCCCTAC 
CACATTGGCC ACTTCTCAGT GGTTGGACTT GGTTTTGAAG AATATGTTCG TGCTTCACAC 
TTTGAGGGGC TCATCACCAC TATTGTCGGA TATGTCCTCT TAGCCATCAC ACTCATTGTG 
TGTCATGGAT TAGCAGCGCT GGTGAGATTT CAAAGATCAC GCCGCTTGTT AGGCGTTTGC 
TACATAGTTG TAAAGGTGTC TCTTCTAGTA GTCGTAGAGA TTGGTGTGTT CCCCCTCATC 
TGCGGCTGGT GGCTTGATAT CTGCTCTTTG GAAATGTTTG ATGCTTCATT AAAAGACAGA 
GAGTTGAGTT TCGAGTCAGC ACCAGGTACC ACTATGTTTC TTCATTGGCT GGTGGGGATG 
GTCTACGTCT TCTATTTTGC ATCTTTTATT CTTCTACTTC GAGAGGTACT GAGGCCTGGA 
GTATTATGGT TTCTCAGAAA TCTGAATGAT CCAGATTTCA ACCCTGTGCA GGAAATGATT 
CACCTGCCAA TATACAGACA CCTGAGACGA TTCATATTAT CTGTGGTTGT GTTTGGCTCA 
ATTGTTCTTC TCATGTTGTG GCTTCCTATC CGGATAATCA AGCACATCTT TCCTTCATTT 
CTACCCTACA ATGTGATGCT TTACAGTGAC GCTCCAGTGA GTGAGTTGTC TCTGGAGCTG 
CTGTTGTTGC AGGTCGTTCT TCCTGCACTG TTGGAACAAG GACACACACG GCAGTGGCTC 
AAAGGTCTAG TGCGAGCCTG GACCGTGACC GCTGGATACT TGCTTGATTT GCACTCGTAC 
TTGCTGGGAG ACCAGGAAGA CAACGAGAAC AATGCCAACC AGCAAGCCAA CAACAATAAC 
CAACAGGCCC GTAACAATGC TATTCCTGTG GTGGGAGAAG GGCTGCACGC TGCCCACCAG 
GCTATACTGC AGCAGGGCGG CCCGGTAGGA TTCCAACCCT ATCATCGGCC TATGAAATTT 
CCTCTAAGGA TTGTCTTGCT GATATTGTTC ATGTGTGTGA CGCTACTTTT GGCCAGTTTA 
GTGTGTCTTA CGTTACCAGT ATTTACAGGA CGCTGGCTGA TGTCCTTCTG GACGGGCAGT 
GCTAAGATCC ACGAGCTGTA CACTGCAGCA TGTGGCCTCT ATGTGTGCTG GCTCTCCATC 
AGAGCGATCA CCGTACTGCT GGCCTGGATG CCTCAGGGTC GCAGAGTCAT CCTGCTCAAG 
ATTCAGGAGT GGACCCTCAT GCTTCAAGAC AGCTCGTGTT TGAAGAATGG AGTCACATGC 
ATTGCTCAGA TTATGAAGAC CCTGATTGTG GCTGTGCTGT TGGCTGGAGT GATTCCTCTG 
CTCTTGGGAC TGCTGTTTGA GCTGGTGATT GTAGCTCCTC TCAGAGTGCC GCTGGATCAG 
ACTCCTTTGT TCTACCCCTG GCAGGACTGG GCTTTAGGAG TGCTTCATGC CAAAATCATT 
GCTGCCATCA CCCTCATGGG TCCCCAGTGG TGGCTGAAGA CCGTTATTGA GCAGGTGTAT 
GCCAATGGAA TCCGGAACAT TGACCTTCAT TTCATCATCA GGAAGCTAGC GGCTCCGGTC 
ATTGCTGTAT TGCTGCTGTC TCTCTGCATC CCTTATGTGA TTGCTGTGGG CATCGTGCCC 
CTGCTTGGGG TCACGATGGA GATGCAGAAT CTTGTGCAGA GGAGAATCTA CCCCTTTCTC 
CTTATGGTGG TGTTTCTGAT GGGGATCCTC TCTTTTCAAA TCCGACAATT CAAGCGCCTT 
TATGAGCACA TCAAGAATGA CAAGTACCTG GTTGGACAGA GACTAGTGAA CTATGAACGG 
AAAGCTGGCA AAGCCAGTGC CAGCACACAC AGCAGTTCAG TTCAGGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

march6 ( XM_003197869.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_003197917.1 Danio rerio membrane-associated ring finger (C3HC4) 6 (march6), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa07101
Clone ID Related Accession (Same CDS sequence)	XM_003197869.4 , XM_003197869.5
Accession Version	XM_003197869.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2784bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase MARCH6 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_003197869.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACTG CCGAAGAAGC TGATATATGT AGGGTGTGTC GATCTGAGGG AACCCAAGAC 
AAACCCCTCT ACCATCCCTG TGTGTGTACT GGAAGTATTA AGTTCATCCA CCAAGAATGC 
TTGGTGCAGT GGCTTAAACA CAGCAGAAAA GAATACTGCG AATTATGCAA GCACAGATTT 
GCTTTCACGC CAATCTACTC TCCAGACATG CCTTCACGGC TCCCAGTGCA GGACATTTTC 
GCAGGACTGG TCACCAGTAT AGGCACAGCA ATCCGATACT GGTTTCATTA CACGCTTGTT 
GCCTTTGCTT GGCTGGGGGT TGTACCATTA ACAGCATGTC GCATCTATAA GTGTCTGTTC 
ACTGGATCTG TAAGCTCGCT CCTGACTCTG CCATTAGACA TGCTCTCCAC AGAGAACTTG 
TTGGCCGACT GCCTGCAGGG CTGCTTTGTG GTGACCTGCA CCCTCTGCGC CTTCATCAGT 
CTGGTGTGGC TGCGGGAGCA GATTGTTCAC GGTGGAGCCC CTTTATGGTT GGAGCAGAAC 
CAGCAGCAAC CTGCCAATGC CGCAGGACCG CCCAATGAGG CCCCTGGCCA GGGTAATGGT 
GGTGCTGAGA ACCAGCCCAT GCCAGCTCCG GCTGAACCTC CAGCAGAAAA CGCTGCAGCT 
GCTGAGGCTC CTGACCTCCC CGCTGATCCT GCTGAAGAGA TGGAGCTTGA TAATGAGGAT 
GAGGATGATG GAGGAGCTGA AGATGTGGCA GATGCTAACA ATGGAGCACA AGATGATATG 
AACTGGAATG CTCTGGAATG GGACCGTGCA GCTGAAGAGC TCACTTGGGA AAGGATGCTT 
GGGCTTGATG GGTCTCTGGT TTTCCTGGAG CATGTGTTCT GGGTGGTCTC ACTCAACACT 
CTCTTCATTT TGGTGTTTGC TTTCTGCCCC TACCACATTG GCCACTTCTC AGTGGTTGGA 
CTTGGTTTTG AAGAATATGT TCGTGCTTCA CACTTTGAGG GGCTCATCAC CACTATTGTC 
GGATATGTCC TCTTAGCCAT CACACTCATT GTGTGTCATG GATTAGCAGC GCTGGTGAGA 
TTTCAAAGAT CACGCCGCTT GTTAGGCGTT TGCTACATAG TTGTAAAGGT GTCTCTTCTA 
GTAGTCGTAG AGATTGGTGT GTTCCCCCTC ATCTGCGGCT GGTGGCTTGA TATCTGCTCT 
TTGGAAATGT TTGATGCTTC ATTAAAAGAC AGAGAGTTGA GTTTCGAGTC AGCACCAGGT 
ACCACTATGT TTCTTCATTG GCTGGTGGGG ATGGTCTACG TCTTCTATTT TGCATCTTTT 
ATTCTTCTAC TTCGAGAGGT ACTGAGGCCT GGAGTATTAT GGTTTCTCAG AAATCTGAAT 
GATCCAGATT TCAACCCTGT GCAGGAAATG ATTCACCTGC CAATATACAG ACACCTGAGA 
CGATTCATAT TATCTGTGGT TGTGTTTGGC TCAATTGTTC TTCTCATGTT GTGGCTTCCT 
ATCCGGATAA TCAAGCACAT CTTTCCTTCA TTTCTACCCT ACAATGTGAT GCTTTACAGT 
GACGCTCCAG TGAGTGAGTT GTCTCTGGAG CTGCTGTTGT TGCAGGTCGT TCTTCCTGCA 
CTGTTGGAAC AAGGACACAC ACGGCAGTGG CTCAAAGGTC TAGTGCGAGC CTGGACCGTG 
ACCGCTGGAT ACTTGCTTGA TTTGCACTCG TACTTGCTGG GAGACCAGGA AGACAACGAG 
AACAATGCCA ACCAGCAAGC CAACAACAAT AACCAACAGG CCCGTAACAA TGCTATTCCT 
GTGGTGGGAG AAGGGCTGCA CGCTGCCCAC CAGGCTATAC TGCAGCAGGG CGGCCCGGTA 
GGATTCCAAC CCTATCATCG GCCTATGAAA TTTCCTCTAA GGATTGTCTT GCTGATATTG 
TTCATGTGTG TGACGCTACT TTTGGCCAGT TTAGTGTGTC TTACGTTACC AGTATTTACA 
GGACGCTGGC TGATGTCCTT CTGGACGGGC AGTGCTAAGA TCCACGAGCT GTACACTGCA 
GCATGTGGCC TCTATGTGTG CTGGCTCTCC ATCAGAGCGA TCACCGTACT GCTGGCCTGG 
ATGCCTCAGG GTCGCAGAGT CATCCTGCTC AAGATTCAGG AGTGGACCCT CATGCTTCAA 
GACAGCTCGT GTTTGAAGAA TGGAGTCACA TGCATTGCTC AGATTATGAA GACCCTGATT 
GTGGCTGTGC TGTTGGCTGG AGTGATTCCT CTGCTCTTGG GACTGCTGTT TGAGCTGGTG 
ATTGTAGCTC CTCTCAGAGT GCCGCTGGAT CAGACTCCTT TGTTCTACCC CTGGCAGGAC 
TGGGCTTTAG GAGTGCTTCA TGCCAAAATC ATTGCTGCCA TCACCCTCAT GGGTCCCCAG 
TGGTGGCTGA AGACCGTTAT TGAGCAGGTG TATGCCAATG GAATCCGGAA CATTGACCTT 
CATTTCATCA TCAGGAAGCT AGCGGCTCCG GTCATTGCTG TATTGCTGCT GTCTCTCTGC 
ATCCCTTATG TGATTGCTGT GGGCATCGTG CCCCTGCTTG GGGTCACGAT GGAGATGCAG 
AATCTTGTGC AGAGGAGAAT CTACCCCTTT CTCCTTATGG TGGTGTTTCT GATGGGGATC 
CTCTCTTTTC AAATCCGACA ATTCAAGCGC CTTTATGAGC ACATCAAGAA TGACAAGTAC 
CTGGTTGGAC AGAGACTAGT GAACTATGAA CGGAAAGCTG GCAAAGCCAG TGCCAGCACA 
CACAGCAGTT CAGTTCAGGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003197917.1
CDS	277..3060
Translation	MDTAEEADICRVCRSEGTQDKPLYHPCVCTGSIKFIHQECLVQWLKHSRKEYCELCKHRFAFTPIYSPDMPSRLPVQDIFAGLVTSIGTAIRYWFHYTLVAFAWLGVVPLTACRIYKCLFTGSVSSLLTLPLDMLSTENLLADCLQGCFVVTCTLCAFISLVWLREQIVHGGAPLWLEQNQQQPANAAGPPNEAPGQGNGGAENQPMPAPAEPPAENAAAAEAPDLPADPAEEMELDNEDEDDGGAEDVADANNGAQDDMNWNALEWDRAAEELTWERMLGLDGSLVFLEHVFWVVSLNTLFILVFAFCPYHIGHFSVVGLGFEEYVRASHFEGLITTIVGYVLLAITLIVCHGLAALVRFQRSRRLLGVCYIVVKVSLLVVVEIGVFPLICGWWLDICSLEMFDASLKDRELSFESAPGTTMFLHWLVGMVYVFYFASFILLLREVLRPGVLWFLRNLNDPDFNPVQEMIHLPIYRHLRRFILSVVVFGSIVLLMLWLPIRIIKHIFPSFLPYNVMLYSDAPVSELSLELLLLQVVLPALLEQGHTRQWLKGLVRAWTVTAGYLLDLHSYLLGDQEDNENNANQQANNNNQQARNNAIPVVGEGLHAAHQAILQQGGPVGFQPYHRPMKFPLRIVLLILFMCVTLLLASLVCLTLPVFTGRWLMSFWTGSAKIHELYTAACGLYVCWLSIRAITVLLAWMPQGRRVILLKIQEWTLMLQDSSCLKNGVTCIAQIMKTLIVAVLLAGVIPLLLGLLFELVIVAPLRVPLDQTPLFYPWQDWALGVLHAKIIAAITLMGPQWWLKTVIEQVYANGIRNIDLHFIIRKLAAPVIAVLLLSLCIPYVIAVGIVPLLGVTMEMQNLVQRRIYPFLLMVVFLMGILSFQIRQFKRLYEHIKNDKYLVGQRLVNYERKAGKASASTHSSSVQE

Target ORF information:

RefSeq Version	XM_003197869.4
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio membrane-associated ring finger (C3HC4) 6 (march6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003197869.4

ORF Insert Sequence:

ATGGACACTG CCGAAGAAGC TGATATATGT AGGGTGTGTC GATCTGAGGG AACCCAAGAC 
AAACCCCTCT ACCATCCCTG TGTGTGTACT GGAAGTATTA AGTTCATCCA CCAAGAATGC 
TTGGTGCAGT GGCTTAAACA CAGCAGAAAA GAATACTGCG AATTATGCAA GCACAGATTT 
GCTTTCACGC CAATCTACTC TCCAGACATG CCTTCACGGC TCCCAGTGCA GGACATTTTC 
GCAGGACTGG TCACCAGTAT AGGCACAGCA ATCCGATACT GGTTTCATTA CACGCTTGTT 
GCCTTTGCTT GGCTGGGGGT TGTACCATTA ACAGCATGTC GCATCTATAA GTGTCTGTTC 
ACTGGATCTG TAAGCTCGCT CCTGACTCTG CCATTAGACA TGCTCTCCAC AGAGAACTTG 
TTGGCCGACT GCCTGCAGGG CTGCTTTGTG GTGACCTGCA CCCTCTGCGC CTTCATCAGT 
CTGGTGTGGC TGCGGGAGCA GATTGTTCAC GGTGGAGCCC CTTTATGGTT GGAGCAGAAC 
CAGCAGCAAC CTGCCAATGC CGCAGGACCG CCCAATGAGG CCCCTGGCCA GGGTAATGGT 
GGTGCTGAGA ACCAGCCCAT GCCAGCTCCG GCTGAACCTC CAGCAGAAAA CGCTGCAGCT 
GCTGAGGCTC CTGACCTCCC CGCTGATCCT GCTGAAGAGA TGGAGCTTGA TAATGAGGAT 
GAGGATGATG GAGGAGCTGA AGATGTGGCA GATGCTAACA ATGGAGCACA AGATGATATG 
AACTGGAATG CTCTGGAATG GGACCGTGCA GCTGAAGAGC TCACTTGGGA AAGGATGCTT 
GGGCTTGATG GGTCTCTGGT TTTCCTGGAG CATGTGTTCT GGGTGGTCTC ACTCAACACT 
CTCTTCATTT TGGTGTTTGC TTTCTGCCCC TACCACATTG GCCACTTCTC AGTGGTTGGA 
CTTGGTTTTG AAGAATATGT TCGTGCTTCA CACTTTGAGG GGCTCATCAC CACTATTGTC 
GGATATGTCC TCTTAGCCAT CACACTCATT GTGTGTCATG GATTAGCAGC GCTGGTGAGA 
TTTCAAAGAT CACGCCGCTT GTTAGGCGTT TGCTACATAG TTGTAAAGGT GTCTCTTCTA 
GTAGTCGTAG AGATTGGTGT GTTCCCCCTC ATCTGCGGCT GGTGGCTTGA TATCTGCTCT 
TTGGAAATGT TTGATGCTTC ATTAAAAGAC AGAGAGTTGA GTTTCGAGTC AGCACCAGGT 
ACCACTATGT TTCTTCATTG GCTGGTGGGG ATGGTCTACG TCTTCTATTT TGCATCTTTT 
ATTCTTCTAC TTCGAGAGGT ACTGAGGCCT GGAGTATTAT GGTTTCTCAG AAATCTGAAT 
GATCCAGATT TCAACCCTGT GCAGGAAATG ATTCACCTGC CAATATACAG ACACCTGAGA 
CGATTCATAT TATCTGTGGT TGTGTTTGGC TCAATTGTTC TTCTCATGTT GTGGCTTCCT 
ATCCGGATAA TCAAGCACAT CTTTCCTTCA TTTCTACCCT ACAATGTGAT GCTTTACAGT 
GACGCTCCAG TGAGTGAGTT GTCTCTGGAG CTGCTGTTGT TGCAGGTCGT TCTTCCTGCA 
CTGTTGGAAC AAGGACACAC ACGGCAGTGG CTCAAAGGTC TAGTGCGAGC CTGGACCGTG 
ACCGCTGGAT ACTTGCTTGA TTTGCACTCG TACTTGCTGG GAGACCAGGA AGACAACGAG 
AACAATGCCA ACCAGCAAGC CAACAACAAT AACCAACAGG CCCGTAACAA TGCTATTCCT 
GTGGTGGGAG AAGGGCTGCA CGCTGCCCAC CAGGCTATAC TGCAGCAGGG CGGCCCGGTA 
GGATTCCAAC CCTATCATCG GCCTATGAAA TTTCCTCTAA GGATTGTCTT GCTGATATTG 
TTCATGTGTG TGACGCTACT TTTGGCCAGT TTAGTGTGTC TTACGTTACC AGTATTTACA 
GGACGCTGGC TGATGTCCTT CTGGACGGGC AGTGCTAAGA TCCACGAGCT GTACACTGCA 
GCATGTGGCC TCTATGTGTG CTGGCTCTCC ATCAGAGCGA TCACCGTACT GCTGGCCTGG 
ATGCCTCAGG GTCGCAGAGT CATCCTGCTC AAGATTCAGG AGTGGACCCT CATGCTTCAA 
GACAGCTCGT GTTTGAAGAA TGGAGTCACA TGCATTGCTC AGATTATGAA GACCCTGATT 
GTGGCTGTGC TGTTGGCTGG AGTGATTCCT CTGCTCTTGG GACTGCTGTT TGAGCTGGTG 
ATTGTAGCTC CTCTCAGAGT GCCGCTGGAT CAGACTCCTT TGTTCTACCC CTGGCAGGAC 
TGGGCTTTAG GAGTGCTTCA TGCCAAAATC ATTGCTGCCA TCACCCTCAT GGGTCCCCAG 
TGGTGGCTGA AGACCGTTAT TGAGCAGGTG TATGCCAATG GAATCCGGAA CATTGACCTT 
CATTTCATCA TCAGGAAGCT AGCGGCTCCG GTCATTGCTG TATTGCTGCT GTCTCTCTGC 
ATCCCTTATG TGATTGCTGT GGGCATCGTG CCCCTGCTTG GGGTCACGAT GGAGATGCAG 
AATCTTGTGC AGAGGAGAAT CTACCCCTTT CTCCTTATGG TGGTGTTTCT GATGGGGATC 
CTCTCTTTTC AAATCCGACA ATTCAAGCGC CTTTATGAGC ACATCAAGAA TGACAAGTAC 
CTGGTTGGAC AGAGACTAGT GAACTATGAA CGGAAAGCTG GCAAAGCCAG TGCCAGCACA 
CACAGCAGTT CAGTTCAGGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

march6 ( XM_003197869.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_003197917.1 Danio rerio membrane-associated ring finger (C3HC4) 6 (march6), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa07101
Clone ID Related Accession (Same CDS sequence)	XM_003197869.4 , XM_003197869.5
Accession Version	XM_003197869.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2784bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase MARCH6 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_003197869.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACTG CCGAAGAAGC TGATATATGT AGGGTGTGTC GATCTGAGGG AACCCAAGAC 
AAACCCCTCT ACCATCCCTG TGTGTGTACT GGAAGTATTA AGTTCATCCA CCAAGAATGC 
TTGGTGCAGT GGCTTAAACA CAGCAGAAAA GAATACTGCG AATTATGCAA GCACAGATTT 
GCTTTCACGC CAATCTACTC TCCAGACATG CCTTCACGGC TCCCAGTGCA GGACATTTTC 
GCAGGACTGG TCACCAGTAT AGGCACAGCA ATCCGATACT GGTTTCATTA CACGCTTGTT 
GCCTTTGCTT GGCTGGGGGT TGTACCATTA ACAGCATGTC GCATCTATAA GTGTCTGTTC 
ACTGGATCTG TAAGCTCGCT CCTGACTCTG CCATTAGACA TGCTCTCCAC AGAGAACTTG 
TTGGCCGACT GCCTGCAGGG CTGCTTTGTG GTGACCTGCA CCCTCTGCGC CTTCATCAGT 
CTGGTGTGGC TGCGGGAGCA GATTGTTCAC GGTGGAGCCC CTTTATGGTT GGAGCAGAAC 
CAGCAGCAAC CTGCCAATGC CGCAGGACCG CCCAATGAGG CCCCTGGCCA GGGTAATGGT 
GGTGCTGAGA ACCAGCCCAT GCCAGCTCCG GCTGAACCTC CAGCAGAAAA CGCTGCAGCT 
GCTGAGGCTC CTGACCTCCC CGCTGATCCT GCTGAAGAGA TGGAGCTTGA TAATGAGGAT 
GAGGATGATG GAGGAGCTGA AGATGTGGCA GATGCTAACA ATGGAGCACA AGATGATATG 
AACTGGAATG CTCTGGAATG GGACCGTGCA GCTGAAGAGC TCACTTGGGA AAGGATGCTT 
GGGCTTGATG GGTCTCTGGT TTTCCTGGAG CATGTGTTCT GGGTGGTCTC ACTCAACACT 
CTCTTCATTT TGGTGTTTGC TTTCTGCCCC TACCACATTG GCCACTTCTC AGTGGTTGGA 
CTTGGTTTTG AAGAATATGT TCGTGCTTCA CACTTTGAGG GGCTCATCAC CACTATTGTC 
GGATATGTCC TCTTAGCCAT CACACTCATT GTGTGTCATG GATTAGCAGC GCTGGTGAGA 
TTTCAAAGAT CACGCCGCTT GTTAGGCGTT TGCTACATAG TTGTAAAGGT GTCTCTTCTA 
GTAGTCGTAG AGATTGGTGT GTTCCCCCTC ATCTGCGGCT GGTGGCTTGA TATCTGCTCT 
TTGGAAATGT TTGATGCTTC ATTAAAAGAC AGAGAGTTGA GTTTCGAGTC AGCACCAGGT 
ACCACTATGT TTCTTCATTG GCTGGTGGGG ATGGTCTACG TCTTCTATTT TGCATCTTTT 
ATTCTTCTAC TTCGAGAGGT ACTGAGGCCT GGAGTATTAT GGTTTCTCAG AAATCTGAAT 
GATCCAGATT TCAACCCTGT GCAGGAAATG ATTCACCTGC CAATATACAG ACACCTGAGA 
CGATTCATAT TATCTGTGGT TGTGTTTGGC TCAATTGTTC TTCTCATGTT GTGGCTTCCT 
ATCCGGATAA TCAAGCACAT CTTTCCTTCA TTTCTACCCT ACAATGTGAT GCTTTACAGT 
GACGCTCCAG TGAGTGAGTT GTCTCTGGAG CTGCTGTTGT TGCAGGTCGT TCTTCCTGCA 
CTGTTGGAAC AAGGACACAC ACGGCAGTGG CTCAAAGGTC TAGTGCGAGC CTGGACCGTG 
ACCGCTGGAT ACTTGCTTGA TTTGCACTCG TACTTGCTGG GAGACCAGGA AGACAACGAG 
AACAATGCCA ACCAGCAAGC CAACAACAAT AACCAACAGG CCCGTAACAA TGCTATTCCT 
GTGGTGGGAG AAGGGCTGCA CGCTGCCCAC CAGGCTATAC TGCAGCAGGG CGGCCCGGTA 
GGATTCCAAC CCTATCATCG GCCTATGAAA TTTCCTCTAA GGATTGTCTT GCTGATATTG 
TTCATGTGTG TGACGCTACT TTTGGCCAGT TTAGTGTGTC TTACGTTACC AGTATTTACA 
GGACGCTGGC TGATGTCCTT CTGGACGGGC AGTGCTAAGA TCCACGAGCT GTACACTGCA 
GCATGTGGCC TCTATGTGTG CTGGCTCTCC ATCAGAGCGA TCACCGTACT GCTGGCCTGG 
ATGCCTCAGG GTCGCAGAGT CATCCTGCTC AAGATTCAGG AGTGGACCCT CATGCTTCAA 
GACAGCTCGT GTTTGAAGAA TGGAGTCACA TGCATTGCTC AGATTATGAA GACCCTGATT 
GTGGCTGTGC TGTTGGCTGG AGTGATTCCT CTGCTCTTGG GACTGCTGTT TGAGCTGGTG 
ATTGTAGCTC CTCTCAGAGT GCCGCTGGAT CAGACTCCTT TGTTCTACCC CTGGCAGGAC 
TGGGCTTTAG GAGTGCTTCA TGCCAAAATC ATTGCTGCCA TCACCCTCAT GGGTCCCCAG 
TGGTGGCTGA AGACCGTTAT TGAGCAGGTG TATGCCAATG GAATCCGGAA CATTGACCTT 
CATTTCATCA TCAGGAAGCT AGCGGCTCCG GTCATTGCTG TATTGCTGCT GTCTCTCTGC 
ATCCCTTATG TGATTGCTGT GGGCATCGTG CCCCTGCTTG GGGTCACGAT GGAGATGCAG 
AATCTTGTGC AGAGGAGAAT CTACCCCTTT CTCCTTATGG TGGTGTTTCT GATGGGGATC 
CTCTCTTTTC AAATCCGACA ATTCAAGCGC CTTTATGAGC ACATCAAGAA TGACAAGTAC 
CTGGTTGGAC AGAGACTAGT GAACTATGAA CGGAAAGCTG GCAAAGCCAG TGCCAGCACA 
CACAGCAGTT CAGTTCAGGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003197917.1
CDS	271..3054
Translation	MDTAEEADICRVCRSEGTQDKPLYHPCVCTGSIKFIHQECLVQWLKHSRKEYCELCKHRFAFTPIYSPDMPSRLPVQDIFAGLVTSIGTAIRYWFHYTLVAFAWLGVVPLTACRIYKCLFTGSVSSLLTLPLDMLSTENLLADCLQGCFVVTCTLCAFISLVWLREQIVHGGAPLWLEQNQQQPANAAGPPNEAPGQGNGGAENQPMPAPAEPPAENAAAAEAPDLPADPAEEMELDNEDEDDGGAEDVADANNGAQDDMNWNALEWDRAAEELTWERMLGLDGSLVFLEHVFWVVSLNTLFILVFAFCPYHIGHFSVVGLGFEEYVRASHFEGLITTIVGYVLLAITLIVCHGLAALVRFQRSRRLLGVCYIVVKVSLLVVVEIGVFPLICGWWLDICSLEMFDASLKDRELSFESAPGTTMFLHWLVGMVYVFYFASFILLLREVLRPGVLWFLRNLNDPDFNPVQEMIHLPIYRHLRRFILSVVVFGSIVLLMLWLPIRIIKHIFPSFLPYNVMLYSDAPVSELSLELLLLQVVLPALLEQGHTRQWLKGLVRAWTVTAGYLLDLHSYLLGDQEDNENNANQQANNNNQQARNNAIPVVGEGLHAAHQAILQQGGPVGFQPYHRPMKFPLRIVLLILFMCVTLLLASLVCLTLPVFTGRWLMSFWTGSAKIHELYTAACGLYVCWLSIRAITVLLAWMPQGRRVILLKIQEWTLMLQDSSCLKNGVTCIAQIMKTLIVAVLLAGVIPLLLGLLFELVIVAPLRVPLDQTPLFYPWQDWALGVLHAKIIAAITLMGPQWWLKTVIEQVYANGIRNIDLHFIIRKLAAPVIAVLLLSLCIPYVIAVGIVPLLGVTMEMQNLVQRRIYPFLLMVVFLMGILSFQIRQFKRLYEHIKNDKYLVGQRLVNYERKAGKASASTHSSSVQE

Target ORF information:

RefSeq Version	XM_003197869.5
Organism	Danio rerio(zebrafish)
Definition	Danio rerio membrane-associated ring finger (C3HC4) 6 (march6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003197869.5

ORF Insert Sequence:

ATGGACACTG CCGAAGAAGC TGATATATGT AGGGTGTGTC GATCTGAGGG AACCCAAGAC 
AAACCCCTCT ACCATCCCTG TGTGTGTACT GGAAGTATTA AGTTCATCCA CCAAGAATGC 
TTGGTGCAGT GGCTTAAACA CAGCAGAAAA GAATACTGCG AATTATGCAA GCACAGATTT 
GCTTTCACGC CAATCTACTC TCCAGACATG CCTTCACGGC TCCCAGTGCA GGACATTTTC 
GCAGGACTGG TCACCAGTAT AGGCACAGCA ATCCGATACT GGTTTCATTA CACGCTTGTT 
GCCTTTGCTT GGCTGGGGGT TGTACCATTA ACAGCATGTC GCATCTATAA GTGTCTGTTC 
ACTGGATCTG TAAGCTCGCT CCTGACTCTG CCATTAGACA TGCTCTCCAC AGAGAACTTG 
TTGGCCGACT GCCTGCAGGG CTGCTTTGTG GTGACCTGCA CCCTCTGCGC CTTCATCAGT 
CTGGTGTGGC TGCGGGAGCA GATTGTTCAC GGTGGAGCCC CTTTATGGTT GGAGCAGAAC 
CAGCAGCAAC CTGCCAATGC CGCAGGACCG CCCAATGAGG CCCCTGGCCA GGGTAATGGT 
GGTGCTGAGA ACCAGCCCAT GCCAGCTCCG GCTGAACCTC CAGCAGAAAA CGCTGCAGCT 
GCTGAGGCTC CTGACCTCCC CGCTGATCCT GCTGAAGAGA TGGAGCTTGA TAATGAGGAT 
GAGGATGATG GAGGAGCTGA AGATGTGGCA GATGCTAACA ATGGAGCACA AGATGATATG 
AACTGGAATG CTCTGGAATG GGACCGTGCA GCTGAAGAGC TCACTTGGGA AAGGATGCTT 
GGGCTTGATG GGTCTCTGGT TTTCCTGGAG CATGTGTTCT GGGTGGTCTC ACTCAACACT 
CTCTTCATTT TGGTGTTTGC TTTCTGCCCC TACCACATTG GCCACTTCTC AGTGGTTGGA 
CTTGGTTTTG AAGAATATGT TCGTGCTTCA CACTTTGAGG GGCTCATCAC CACTATTGTC 
GGATATGTCC TCTTAGCCAT CACACTCATT GTGTGTCATG GATTAGCAGC GCTGGTGAGA 
TTTCAAAGAT CACGCCGCTT GTTAGGCGTT TGCTACATAG TTGTAAAGGT GTCTCTTCTA 
GTAGTCGTAG AGATTGGTGT GTTCCCCCTC ATCTGCGGCT GGTGGCTTGA TATCTGCTCT 
TTGGAAATGT TTGATGCTTC ATTAAAAGAC AGAGAGTTGA GTTTCGAGTC AGCACCAGGT 
ACCACTATGT TTCTTCATTG GCTGGTGGGG ATGGTCTACG TCTTCTATTT TGCATCTTTT 
ATTCTTCTAC TTCGAGAGGT ACTGAGGCCT GGAGTATTAT GGTTTCTCAG AAATCTGAAT 
GATCCAGATT TCAACCCTGT GCAGGAAATG ATTCACCTGC CAATATACAG ACACCTGAGA 
CGATTCATAT TATCTGTGGT TGTGTTTGGC TCAATTGTTC TTCTCATGTT GTGGCTTCCT 
ATCCGGATAA TCAAGCACAT CTTTCCTTCA TTTCTACCCT ACAATGTGAT GCTTTACAGT 
GACGCTCCAG TGAGTGAGTT GTCTCTGGAG CTGCTGTTGT TGCAGGTCGT TCTTCCTGCA 
CTGTTGGAAC AAGGACACAC ACGGCAGTGG CTCAAAGGTC TAGTGCGAGC CTGGACCGTG 
ACCGCTGGAT ACTTGCTTGA TTTGCACTCG TACTTGCTGG GAGACCAGGA AGACAACGAG 
AACAATGCCA ACCAGCAAGC CAACAACAAT AACCAACAGG CCCGTAACAA TGCTATTCCT 
GTGGTGGGAG AAGGGCTGCA CGCTGCCCAC CAGGCTATAC TGCAGCAGGG CGGCCCGGTA 
GGATTCCAAC CCTATCATCG GCCTATGAAA TTTCCTCTAA GGATTGTCTT GCTGATATTG 
TTCATGTGTG TGACGCTACT TTTGGCCAGT TTAGTGTGTC TTACGTTACC AGTATTTACA 
GGACGCTGGC TGATGTCCTT CTGGACGGGC AGTGCTAAGA TCCACGAGCT GTACACTGCA 
GCATGTGGCC TCTATGTGTG CTGGCTCTCC ATCAGAGCGA TCACCGTACT GCTGGCCTGG 
ATGCCTCAGG GTCGCAGAGT CATCCTGCTC AAGATTCAGG AGTGGACCCT CATGCTTCAA 
GACAGCTCGT GTTTGAAGAA TGGAGTCACA TGCATTGCTC AGATTATGAA GACCCTGATT 
GTGGCTGTGC TGTTGGCTGG AGTGATTCCT CTGCTCTTGG GACTGCTGTT TGAGCTGGTG 
ATTGTAGCTC CTCTCAGAGT GCCGCTGGAT CAGACTCCTT TGTTCTACCC CTGGCAGGAC 
TGGGCTTTAG GAGTGCTTCA TGCCAAAATC ATTGCTGCCA TCACCCTCAT GGGTCCCCAG 
TGGTGGCTGA AGACCGTTAT TGAGCAGGTG TATGCCAATG GAATCCGGAA CATTGACCTT 
CATTTCATCA TCAGGAAGCT AGCGGCTCCG GTCATTGCTG TATTGCTGCT GTCTCTCTGC 
ATCCCTTATG TGATTGCTGT GGGCATCGTG CCCCTGCTTG GGGTCACGAT GGAGATGCAG 
AATCTTGTGC AGAGGAGAAT CTACCCCTTT CTCCTTATGG TGGTGTTTCT GATGGGGATC 
CTCTCTTTTC AAATCCGACA ATTCAAGCGC CTTTATGAGC ACATCAAGAA TGACAAGTAC 
CTGGTTGGAC AGAGACTAGT GAACTATGAA CGGAAAGCTG GCAAAGCCAG TGCCAGCACA 
CACAGCAGTT CAGTTCAGGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

march6 ( XM_005163450.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005163507.1 Danio rerio membrane-associated ring finger (C3HC4) 6 (march6), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa07102
Clone ID Related Accession (Same CDS sequence)	XM_005163450.3 , XM_005163450.4
Accession Version	XM_005163450.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2763bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase MARCH6 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005163450.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACTG CCGAAGAAGC TGATATATGT AGGGTGTGTC GATCTGAGGG AACCCAAGAC 
AAACCCCTCT ACCATCCCTG TGTGTGTACT GGAAGTATTA AGTTCATCCA CCAAGAATGC 
TTGGTGCAGT GGCTTAAACA CAGCAGAAAA GAATACTGCG AATTATGCAA GCACAGATTT 
GCTTTCACGC CAATCTACTC TCCAGACATG CCTTCACGGC TCCCAGTGCA GGACATTTTC 
GCAGGACTGG TCACCAGTAT AGGCACAGCA ATCCGATACT GGTTTCATTA CACGCTTGTT 
GCCTTTGCTT GGCTGGGGGT TGTACCATTA ACAGCATGTC GCATCTATAA GTGTCTGTTC 
ACTGGATCTG TAAGCTCGCT CCTGACTCTG CCATTAGACA TGCTCTCCAC GTACGTACCA 
GACAGGACAG CTCTCAGAGA GAACTTGTTG GCCGACTGCC TGCAGGGCTG CTTTGTGGTG 
ACCTGCACCC TCTGCGCCTT CATCAGTCTG GTGTGGCTGC GGGAGCAGAT TGTTCACGGT 
GGAGCCCCTT TATGGTTGGA GCAGAACCAG CAGCAACCTG CCAATGCCGC AGGACCGCCC 
AATGAGGCCC CTGGCCAGGG TAATGGTGGT GCTGAGAACC AGCCCATGCC AGCTCCGGCT 
GAACCTCCAG CAGAAAACGC TGCAGCTGCT GAGGCTCCTG ACCTCCCCGC TGATCCTGCT 
GAAGAGATGG AGCTTGATAA TGAGGATGAG GATGATGGAG GAGCTGAAGA TGTGGCAGAT 
GCTAACAATG GAGCACAAGA TGATATGAAC TGGAATGCTC TGGAATGGGA CCGTGCAGCT 
GAAGAGCTCA CTTGGGAAAG GATGCTTGGG CTTGATGGGT CTCTGGTTTT CCTGGAGCAT 
GTGTTCTGGG TGGTCTCACT CAACACTCTC TTCATTTTGG TGTTTGCTTT CTGCCCCTAC 
CACATTGGCC ACTTCTCAGT GGTTGGACTT GGTTTTGAAG AATATGTTCG TGCTTCACAC 
TTTGAGGGGC TCATCACCAC TATTGTCGGA TATGTCCTCT TAGCCATCAC ACTCATTGTG 
TGTCATGGAT TAGCAGCGCT GGTGAGATTT CAAAGATCAC GCCGCTTGTT AGGCGTTTGC 
TACATAGTTG TAAAGGTGTC TCTTCTAGTA GTCGTAGAGA TTGGTGTGTT CCCCCTCATC 
TGCGGCTGGT GGCTTGATAT CTGCTCTTTG GAAATGTTTG ATGCTTCATT AAAAGACAGA 
GAGTTGAGTT TCGAGTCAGC ACCAGGTACC ACTATGTTTC TTCATTGGCT GGTGGGGATG 
GTCTACGTCT TCTATTTTGC ATCTTTTATT CTTCTACTTC GAGAGGTACT GAGGCCTGGA 
GTATTATGGT TTCTCAGAAA TCTGAATGAT CCAGATTTCA ACCCTGTGCA GGAAATGATT 
CACCTGCCAA TATACAGACA CCTGAGACGA TTCATATTAT CTGTGGTTGT GTTTGGCTCA 
ATTGTTCTTC TCATGTTGTG GCTTCCTATC CGGATAATCA AGCACATCTT TCCTTCATTT 
CTACCCTACA ATGTGATGCT TTACAGTGAC GCTCCAGTGA GTGAGTTGTC TCTGGAGCTG 
CTGTTGTTGC AGGTCGTTCT TCCTGCACTG TTGGAACAAG GACACACACG GCAGTGGCTC 
AAAGGTCTAG TGCGAGCCTG GACCGTGACC GCTGGATACT TGCTTGATTT GCACTCGTAC 
TTGCTGGGAG ACCAGGAAGA CAACGAGAAC AATGCCAACC AGCAAGCCAA CAACAATAAC 
CAACAGGCCC GTAACAATGC TATTCCTGTG GTGGGAGAAG GGCTGCACGC TGCCCACCAG 
GCTATACTGC AGCAGGGCGG CCCGGTAGGA TTCCAACCCT ATCATCGGCC TATGAAATTT 
CCTCTAAGGA TTGTCTTGCT GATATTGTTC ATGTGTGTGA CGCTACTTTT GGCCAGTTTA 
GTGTGTCTTA CGTTACCAGT ATTTACAGGA CGCTGGCTGA TGTCCTTCTG GACGGGCAGT 
GCTAAGATCC ACGAGCTGTA CACTGCAGCA TGTGGCCTCT ATGTGTGCTG GCTCTCCATC 
AGAGCGATCA CCGTACTGCT GGCCTGGATG CCTCAGGGTC GCAGAGTCAT CCTGCTCAAG 
ATTCAGGAGT GGACCCTCAT GATTATGAAG ACCCTGATTG TGGCTGTGCT GTTGGCTGGA 
GTGATTCCTC TGCTCTTGGG ACTGCTGTTT GAGCTGGTGA TTGTAGCTCC TCTCAGAGTG 
CCGCTGGATC AGACTCCTTT GTTCTACCCC TGGCAGGACT GGGCTTTAGG AGTGCTTCAT 
GCCAAAATCA TTGCTGCCAT CACCCTCATG GGTCCCCAGT GGTGGCTGAA GACCGTTATT 
GAGCAGGTGT ATGCCAATGG AATCCGGAAC ATTGACCTTC ATTTCATCAT CAGGAAGCTA 
GCGGCTCCGG TCATTGCTGT ATTGCTGCTG TCTCTCTGCA TCCCTTATGT GATTGCTGTG 
GGCATCGTGC CCCTGCTTGG GGTCACGATG GAGATGCAGA ATCTTGTGCA GAGGAGAATC 
TACCCCTTTC TCCTTATGGT GGTGTTTCTG ATGGGGATCC TCTCTTTTCA AATCCGACAA 
TTCAAGCGCC TTTATGAGCA CATCAAGAAT GACAAGTACC TGGTTGGACA GAGACTAGTG 
AACTATGAAC GGAAAGCTGG CAAAGCCAGT GCCAGCACAC ACAGCAGTTC AGTTCAGGAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163507.1
CDS	276..3038
Translation	MDTAEEADICRVCRSEGTQDKPLYHPCVCTGSIKFIHQECLVQWLKHSRKEYCELCKHRFAFTPIYSPDMPSRLPVQDIFAGLVTSIGTAIRYWFHYTLVAFAWLGVVPLTACRIYKCLFTGSVSSLLTLPLDMLSTYVPDRTALRENLLADCLQGCFVVTCTLCAFISLVWLREQIVHGGAPLWLEQNQQQPANAAGPPNEAPGQGNGGAENQPMPAPAEPPAENAAAAEAPDLPADPAEEMELDNEDEDDGGAEDVADANNGAQDDMNWNALEWDRAAEELTWERMLGLDGSLVFLEHVFWVVSLNTLFILVFAFCPYHIGHFSVVGLGFEEYVRASHFEGLITTIVGYVLLAITLIVCHGLAALVRFQRSRRLLGVCYIVVKVSLLVVVEIGVFPLICGWWLDICSLEMFDASLKDRELSFESAPGTTMFLHWLVGMVYVFYFASFILLLREVLRPGVLWFLRNLNDPDFNPVQEMIHLPIYRHLRRFILSVVVFGSIVLLMLWLPIRIIKHIFPSFLPYNVMLYSDAPVSELSLELLLLQVVLPALLEQGHTRQWLKGLVRAWTVTAGYLLDLHSYLLGDQEDNENNANQQANNNNQQARNNAIPVVGEGLHAAHQAILQQGGPVGFQPYHRPMKFPLRIVLLILFMCVTLLLASLVCLTLPVFTGRWLMSFWTGSAKIHELYTAACGLYVCWLSIRAITVLLAWMPQGRRVILLKIQEWTLMIMKTLIVAVLLAGVIPLLLGLLFELVIVAPLRVPLDQTPLFYPWQDWALGVLHAKIIAAITLMGPQWWLKTVIEQVYANGIRNIDLHFIIRKLAAPVIAVLLLSLCIPYVIAVGIVPLLGVTMEMQNLVQRRIYPFLLMVVFLMGILSFQIRQFKRLYEHIKNDKYLVGQRLVNYERKAGKASASTHSSSVQE

Target ORF information:

RefSeq Version	XM_005163450.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio membrane-associated ring finger (C3HC4) 6 (march6), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163450.3

ORF Insert Sequence:

ATGGACACTG CCGAAGAAGC TGATATATGT AGGGTGTGTC GATCTGAGGG AACCCAAGAC 
AAACCCCTCT ACCATCCCTG TGTGTGTACT GGAAGTATTA AGTTCATCCA CCAAGAATGC 
TTGGTGCAGT GGCTTAAACA CAGCAGAAAA GAATACTGCG AATTATGCAA GCACAGATTT 
GCTTTCACGC CAATCTACTC TCCAGACATG CCTTCACGGC TCCCAGTGCA GGACATTTTC 
GCAGGACTGG TCACCAGTAT AGGCACAGCA ATCCGATACT GGTTTCATTA CACGCTTGTT 
GCCTTTGCTT GGCTGGGGGT TGTACCATTA ACAGCATGTC GCATCTATAA GTGTCTGTTC 
ACTGGATCTG TAAGCTCGCT CCTGACTCTG CCATTAGACA TGCTCTCCAC GTACGTACCA 
GACAGGACAG CTCTCAGAGA GAACTTGTTG GCCGACTGCC TGCAGGGCTG CTTTGTGGTG 
ACCTGCACCC TCTGCGCCTT CATCAGTCTG GTGTGGCTGC GGGAGCAGAT TGTTCACGGT 
GGAGCCCCTT TATGGTTGGA GCAGAACCAG CAGCAACCTG CCAATGCCGC AGGACCGCCC 
AATGAGGCCC CTGGCCAGGG TAATGGTGGT GCTGAGAACC AGCCCATGCC AGCTCCGGCT 
GAACCTCCAG CAGAAAACGC TGCAGCTGCT GAGGCTCCTG ACCTCCCCGC TGATCCTGCT 
GAAGAGATGG AGCTTGATAA TGAGGATGAG GATGATGGAG GAGCTGAAGA TGTGGCAGAT 
GCTAACAATG GAGCACAAGA TGATATGAAC TGGAATGCTC TGGAATGGGA CCGTGCAGCT 
GAAGAGCTCA CTTGGGAAAG GATGCTTGGG CTTGATGGGT CTCTGGTTTT CCTGGAGCAT 
GTGTTCTGGG TGGTCTCACT CAACACTCTC TTCATTTTGG TGTTTGCTTT CTGCCCCTAC 
CACATTGGCC ACTTCTCAGT GGTTGGACTT GGTTTTGAAG AATATGTTCG TGCTTCACAC 
TTTGAGGGGC TCATCACCAC TATTGTCGGA TATGTCCTCT TAGCCATCAC ACTCATTGTG 
TGTCATGGAT TAGCAGCGCT GGTGAGATTT CAAAGATCAC GCCGCTTGTT AGGCGTTTGC 
TACATAGTTG TAAAGGTGTC TCTTCTAGTA GTCGTAGAGA TTGGTGTGTT CCCCCTCATC 
TGCGGCTGGT GGCTTGATAT CTGCTCTTTG GAAATGTTTG ATGCTTCATT AAAAGACAGA 
GAGTTGAGTT TCGAGTCAGC ACCAGGTACC ACTATGTTTC TTCATTGGCT GGTGGGGATG 
GTCTACGTCT TCTATTTTGC ATCTTTTATT CTTCTACTTC GAGAGGTACT GAGGCCTGGA 
GTATTATGGT TTCTCAGAAA TCTGAATGAT CCAGATTTCA ACCCTGTGCA GGAAATGATT 
CACCTGCCAA TATACAGACA CCTGAGACGA TTCATATTAT CTGTGGTTGT GTTTGGCTCA 
ATTGTTCTTC TCATGTTGTG GCTTCCTATC CGGATAATCA AGCACATCTT TCCTTCATTT 
CTACCCTACA ATGTGATGCT TTACAGTGAC GCTCCAGTGA GTGAGTTGTC TCTGGAGCTG 
CTGTTGTTGC AGGTCGTTCT TCCTGCACTG TTGGAACAAG GACACACACG GCAGTGGCTC 
AAAGGTCTAG TGCGAGCCTG GACCGTGACC GCTGGATACT TGCTTGATTT GCACTCGTAC 
TTGCTGGGAG ACCAGGAAGA CAACGAGAAC AATGCCAACC AGCAAGCCAA CAACAATAAC 
CAACAGGCCC GTAACAATGC TATTCCTGTG GTGGGAGAAG GGCTGCACGC TGCCCACCAG 
GCTATACTGC AGCAGGGCGG CCCGGTAGGA TTCCAACCCT ATCATCGGCC TATGAAATTT 
CCTCTAAGGA TTGTCTTGCT GATATTGTTC ATGTGTGTGA CGCTACTTTT GGCCAGTTTA 
GTGTGTCTTA CGTTACCAGT ATTTACAGGA CGCTGGCTGA TGTCCTTCTG GACGGGCAGT 
GCTAAGATCC ACGAGCTGTA CACTGCAGCA TGTGGCCTCT ATGTGTGCTG GCTCTCCATC 
AGAGCGATCA CCGTACTGCT GGCCTGGATG CCTCAGGGTC GCAGAGTCAT CCTGCTCAAG 
ATTCAGGAGT GGACCCTCAT GATTATGAAG ACCCTGATTG TGGCTGTGCT GTTGGCTGGA 
GTGATTCCTC TGCTCTTGGG ACTGCTGTTT GAGCTGGTGA TTGTAGCTCC TCTCAGAGTG 
CCGCTGGATC AGACTCCTTT GTTCTACCCC TGGCAGGACT GGGCTTTAGG AGTGCTTCAT 
GCCAAAATCA TTGCTGCCAT CACCCTCATG GGTCCCCAGT GGTGGCTGAA GACCGTTATT 
GAGCAGGTGT ATGCCAATGG AATCCGGAAC ATTGACCTTC ATTTCATCAT CAGGAAGCTA 
GCGGCTCCGG TCATTGCTGT ATTGCTGCTG TCTCTCTGCA TCCCTTATGT GATTGCTGTG 
GGCATCGTGC CCCTGCTTGG GGTCACGATG GAGATGCAGA ATCTTGTGCA GAGGAGAATC 
TACCCCTTTC TCCTTATGGT GGTGTTTCTG ATGGGGATCC TCTCTTTTCA AATCCGACAA 
TTCAAGCGCC TTTATGAGCA CATCAAGAAT GACAAGTACC TGGTTGGACA GAGACTAGTG 
AACTATGAAC GGAAAGCTGG CAAAGCCAGT GCCAGCACAC ACAGCAGTTC AGTTCAGGAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

march6 ( XM_005163450.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005163507.1 Danio rerio membrane-associated ring finger (C3HC4) 6 (march6), transcript variant X3, mRNA.

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CloneID	ODa07102
Clone ID Related Accession (Same CDS sequence)	XM_005163450.3 , XM_005163450.4
Accession Version	XM_005163450.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2763bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase MARCH6 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005163450.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACTG CCGAAGAAGC TGATATATGT AGGGTGTGTC GATCTGAGGG AACCCAAGAC 
AAACCCCTCT ACCATCCCTG TGTGTGTACT GGAAGTATTA AGTTCATCCA CCAAGAATGC 
TTGGTGCAGT GGCTTAAACA CAGCAGAAAA GAATACTGCG AATTATGCAA GCACAGATTT 
GCTTTCACGC CAATCTACTC TCCAGACATG CCTTCACGGC TCCCAGTGCA GGACATTTTC 
GCAGGACTGG TCACCAGTAT AGGCACAGCA ATCCGATACT GGTTTCATTA CACGCTTGTT 
GCCTTTGCTT GGCTGGGGGT TGTACCATTA ACAGCATGTC GCATCTATAA GTGTCTGTTC 
ACTGGATCTG TAAGCTCGCT CCTGACTCTG CCATTAGACA TGCTCTCCAC GTACGTACCA 
GACAGGACAG CTCTCAGAGA GAACTTGTTG GCCGACTGCC TGCAGGGCTG CTTTGTGGTG 
ACCTGCACCC TCTGCGCCTT CATCAGTCTG GTGTGGCTGC GGGAGCAGAT TGTTCACGGT 
GGAGCCCCTT TATGGTTGGA GCAGAACCAG CAGCAACCTG CCAATGCCGC AGGACCGCCC 
AATGAGGCCC CTGGCCAGGG TAATGGTGGT GCTGAGAACC AGCCCATGCC AGCTCCGGCT 
GAACCTCCAG CAGAAAACGC TGCAGCTGCT GAGGCTCCTG ACCTCCCCGC TGATCCTGCT 
GAAGAGATGG AGCTTGATAA TGAGGATGAG GATGATGGAG GAGCTGAAGA TGTGGCAGAT 
GCTAACAATG GAGCACAAGA TGATATGAAC TGGAATGCTC TGGAATGGGA CCGTGCAGCT 
GAAGAGCTCA CTTGGGAAAG GATGCTTGGG CTTGATGGGT CTCTGGTTTT CCTGGAGCAT 
GTGTTCTGGG TGGTCTCACT CAACACTCTC TTCATTTTGG TGTTTGCTTT CTGCCCCTAC 
CACATTGGCC ACTTCTCAGT GGTTGGACTT GGTTTTGAAG AATATGTTCG TGCTTCACAC 
TTTGAGGGGC TCATCACCAC TATTGTCGGA TATGTCCTCT TAGCCATCAC ACTCATTGTG 
TGTCATGGAT TAGCAGCGCT GGTGAGATTT CAAAGATCAC GCCGCTTGTT AGGCGTTTGC 
TACATAGTTG TAAAGGTGTC TCTTCTAGTA GTCGTAGAGA TTGGTGTGTT CCCCCTCATC 
TGCGGCTGGT GGCTTGATAT CTGCTCTTTG GAAATGTTTG ATGCTTCATT AAAAGACAGA 
GAGTTGAGTT TCGAGTCAGC ACCAGGTACC ACTATGTTTC TTCATTGGCT GGTGGGGATG 
GTCTACGTCT TCTATTTTGC ATCTTTTATT CTTCTACTTC GAGAGGTACT GAGGCCTGGA 
GTATTATGGT TTCTCAGAAA TCTGAATGAT CCAGATTTCA ACCCTGTGCA GGAAATGATT 
CACCTGCCAA TATACAGACA CCTGAGACGA TTCATATTAT CTGTGGTTGT GTTTGGCTCA 
ATTGTTCTTC TCATGTTGTG GCTTCCTATC CGGATAATCA AGCACATCTT TCCTTCATTT 
CTACCCTACA ATGTGATGCT TTACAGTGAC GCTCCAGTGA GTGAGTTGTC TCTGGAGCTG 
CTGTTGTTGC AGGTCGTTCT TCCTGCACTG TTGGAACAAG GACACACACG GCAGTGGCTC 
AAAGGTCTAG TGCGAGCCTG GACCGTGACC GCTGGATACT TGCTTGATTT GCACTCGTAC 
TTGCTGGGAG ACCAGGAAGA CAACGAGAAC AATGCCAACC AGCAAGCCAA CAACAATAAC 
CAACAGGCCC GTAACAATGC TATTCCTGTG GTGGGAGAAG GGCTGCACGC TGCCCACCAG 
GCTATACTGC AGCAGGGCGG CCCGGTAGGA TTCCAACCCT ATCATCGGCC TATGAAATTT 
CCTCTAAGGA TTGTCTTGCT GATATTGTTC ATGTGTGTGA CGCTACTTTT GGCCAGTTTA 
GTGTGTCTTA CGTTACCAGT ATTTACAGGA CGCTGGCTGA TGTCCTTCTG GACGGGCAGT 
GCTAAGATCC ACGAGCTGTA CACTGCAGCA TGTGGCCTCT ATGTGTGCTG GCTCTCCATC 
AGAGCGATCA CCGTACTGCT GGCCTGGATG CCTCAGGGTC GCAGAGTCAT CCTGCTCAAG 
ATTCAGGAGT GGACCCTCAT GATTATGAAG ACCCTGATTG TGGCTGTGCT GTTGGCTGGA 
GTGATTCCTC TGCTCTTGGG ACTGCTGTTT GAGCTGGTGA TTGTAGCTCC TCTCAGAGTG 
CCGCTGGATC AGACTCCTTT GTTCTACCCC TGGCAGGACT GGGCTTTAGG AGTGCTTCAT 
GCCAAAATCA TTGCTGCCAT CACCCTCATG GGTCCCCAGT GGTGGCTGAA GACCGTTATT 
GAGCAGGTGT ATGCCAATGG AATCCGGAAC ATTGACCTTC ATTTCATCAT CAGGAAGCTA 
GCGGCTCCGG TCATTGCTGT ATTGCTGCTG TCTCTCTGCA TCCCTTATGT GATTGCTGTG 
GGCATCGTGC CCCTGCTTGG GGTCACGATG GAGATGCAGA ATCTTGTGCA GAGGAGAATC 
TACCCCTTTC TCCTTATGGT GGTGTTTCTG ATGGGGATCC TCTCTTTTCA AATCCGACAA 
TTCAAGCGCC TTTATGAGCA CATCAAGAAT GACAAGTACC TGGTTGGACA GAGACTAGTG 
AACTATGAAC GGAAAGCTGG CAAAGCCAGT GCCAGCACAC ACAGCAGTTC AGTTCAGGAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163507.1
CDS	271..3033
Translation	MDTAEEADICRVCRSEGTQDKPLYHPCVCTGSIKFIHQECLVQWLKHSRKEYCELCKHRFAFTPIYSPDMPSRLPVQDIFAGLVTSIGTAIRYWFHYTLVAFAWLGVVPLTACRIYKCLFTGSVSSLLTLPLDMLSTYVPDRTALRENLLADCLQGCFVVTCTLCAFISLVWLREQIVHGGAPLWLEQNQQQPANAAGPPNEAPGQGNGGAENQPMPAPAEPPAENAAAAEAPDLPADPAEEMELDNEDEDDGGAEDVADANNGAQDDMNWNALEWDRAAEELTWERMLGLDGSLVFLEHVFWVVSLNTLFILVFAFCPYHIGHFSVVGLGFEEYVRASHFEGLITTIVGYVLLAITLIVCHGLAALVRFQRSRRLLGVCYIVVKVSLLVVVEIGVFPLICGWWLDICSLEMFDASLKDRELSFESAPGTTMFLHWLVGMVYVFYFASFILLLREVLRPGVLWFLRNLNDPDFNPVQEMIHLPIYRHLRRFILSVVVFGSIVLLMLWLPIRIIKHIFPSFLPYNVMLYSDAPVSELSLELLLLQVVLPALLEQGHTRQWLKGLVRAWTVTAGYLLDLHSYLLGDQEDNENNANQQANNNNQQARNNAIPVVGEGLHAAHQAILQQGGPVGFQPYHRPMKFPLRIVLLILFMCVTLLLASLVCLTLPVFTGRWLMSFWTGSAKIHELYTAACGLYVCWLSIRAITVLLAWMPQGRRVILLKIQEWTLMIMKTLIVAVLLAGVIPLLLGLLFELVIVAPLRVPLDQTPLFYPWQDWALGVLHAKIIAAITLMGPQWWLKTVIEQVYANGIRNIDLHFIIRKLAAPVIAVLLLSLCIPYVIAVGIVPLLGVTMEMQNLVQRRIYPFLLMVVFLMGILSFQIRQFKRLYEHIKNDKYLVGQRLVNYERKAGKASASTHSSSVQE

Target ORF information:

RefSeq Version	XM_005163450.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio membrane-associated ring finger (C3HC4) 6 (march6), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163450.4

ORF Insert Sequence:

ATGGACACTG CCGAAGAAGC TGATATATGT AGGGTGTGTC GATCTGAGGG AACCCAAGAC 
AAACCCCTCT ACCATCCCTG TGTGTGTACT GGAAGTATTA AGTTCATCCA CCAAGAATGC 
TTGGTGCAGT GGCTTAAACA CAGCAGAAAA GAATACTGCG AATTATGCAA GCACAGATTT 
GCTTTCACGC CAATCTACTC TCCAGACATG CCTTCACGGC TCCCAGTGCA GGACATTTTC 
GCAGGACTGG TCACCAGTAT AGGCACAGCA ATCCGATACT GGTTTCATTA CACGCTTGTT 
GCCTTTGCTT GGCTGGGGGT TGTACCATTA ACAGCATGTC GCATCTATAA GTGTCTGTTC 
ACTGGATCTG TAAGCTCGCT CCTGACTCTG CCATTAGACA TGCTCTCCAC GTACGTACCA 
GACAGGACAG CTCTCAGAGA GAACTTGTTG GCCGACTGCC TGCAGGGCTG CTTTGTGGTG 
ACCTGCACCC TCTGCGCCTT CATCAGTCTG GTGTGGCTGC GGGAGCAGAT TGTTCACGGT 
GGAGCCCCTT TATGGTTGGA GCAGAACCAG CAGCAACCTG CCAATGCCGC AGGACCGCCC 
AATGAGGCCC CTGGCCAGGG TAATGGTGGT GCTGAGAACC AGCCCATGCC AGCTCCGGCT 
GAACCTCCAG CAGAAAACGC TGCAGCTGCT GAGGCTCCTG ACCTCCCCGC TGATCCTGCT 
GAAGAGATGG AGCTTGATAA TGAGGATGAG GATGATGGAG GAGCTGAAGA TGTGGCAGAT 
GCTAACAATG GAGCACAAGA TGATATGAAC TGGAATGCTC TGGAATGGGA CCGTGCAGCT 
GAAGAGCTCA CTTGGGAAAG GATGCTTGGG CTTGATGGGT CTCTGGTTTT CCTGGAGCAT 
GTGTTCTGGG TGGTCTCACT CAACACTCTC TTCATTTTGG TGTTTGCTTT CTGCCCCTAC 
CACATTGGCC ACTTCTCAGT GGTTGGACTT GGTTTTGAAG AATATGTTCG TGCTTCACAC 
TTTGAGGGGC TCATCACCAC TATTGTCGGA TATGTCCTCT TAGCCATCAC ACTCATTGTG 
TGTCATGGAT TAGCAGCGCT GGTGAGATTT CAAAGATCAC GCCGCTTGTT AGGCGTTTGC 
TACATAGTTG TAAAGGTGTC TCTTCTAGTA GTCGTAGAGA TTGGTGTGTT CCCCCTCATC 
TGCGGCTGGT GGCTTGATAT CTGCTCTTTG GAAATGTTTG ATGCTTCATT AAAAGACAGA 
GAGTTGAGTT TCGAGTCAGC ACCAGGTACC ACTATGTTTC TTCATTGGCT GGTGGGGATG 
GTCTACGTCT TCTATTTTGC ATCTTTTATT CTTCTACTTC GAGAGGTACT GAGGCCTGGA 
GTATTATGGT TTCTCAGAAA TCTGAATGAT CCAGATTTCA ACCCTGTGCA GGAAATGATT 
CACCTGCCAA TATACAGACA CCTGAGACGA TTCATATTAT CTGTGGTTGT GTTTGGCTCA 
ATTGTTCTTC TCATGTTGTG GCTTCCTATC CGGATAATCA AGCACATCTT TCCTTCATTT 
CTACCCTACA ATGTGATGCT TTACAGTGAC GCTCCAGTGA GTGAGTTGTC TCTGGAGCTG 
CTGTTGTTGC AGGTCGTTCT TCCTGCACTG TTGGAACAAG GACACACACG GCAGTGGCTC 
AAAGGTCTAG TGCGAGCCTG GACCGTGACC GCTGGATACT TGCTTGATTT GCACTCGTAC 
TTGCTGGGAG ACCAGGAAGA CAACGAGAAC AATGCCAACC AGCAAGCCAA CAACAATAAC 
CAACAGGCCC GTAACAATGC TATTCCTGTG GTGGGAGAAG GGCTGCACGC TGCCCACCAG 
GCTATACTGC AGCAGGGCGG CCCGGTAGGA TTCCAACCCT ATCATCGGCC TATGAAATTT 
CCTCTAAGGA TTGTCTTGCT GATATTGTTC ATGTGTGTGA CGCTACTTTT GGCCAGTTTA 
GTGTGTCTTA CGTTACCAGT ATTTACAGGA CGCTGGCTGA TGTCCTTCTG GACGGGCAGT 
GCTAAGATCC ACGAGCTGTA CACTGCAGCA TGTGGCCTCT ATGTGTGCTG GCTCTCCATC 
AGAGCGATCA CCGTACTGCT GGCCTGGATG CCTCAGGGTC GCAGAGTCAT CCTGCTCAAG 
ATTCAGGAGT GGACCCTCAT GATTATGAAG ACCCTGATTG TGGCTGTGCT GTTGGCTGGA 
GTGATTCCTC TGCTCTTGGG ACTGCTGTTT GAGCTGGTGA TTGTAGCTCC TCTCAGAGTG 
CCGCTGGATC AGACTCCTTT GTTCTACCCC TGGCAGGACT GGGCTTTAGG AGTGCTTCAT 
GCCAAAATCA TTGCTGCCAT CACCCTCATG GGTCCCCAGT GGTGGCTGAA GACCGTTATT 
GAGCAGGTGT ATGCCAATGG AATCCGGAAC ATTGACCTTC ATTTCATCAT CAGGAAGCTA 
GCGGCTCCGG TCATTGCTGT ATTGCTGCTG TCTCTCTGCA TCCCTTATGT GATTGCTGTG 
GGCATCGTGC CCCTGCTTGG GGTCACGATG GAGATGCAGA ATCTTGTGCA GAGGAGAATC 
TACCCCTTTC TCCTTATGGT GGTGTTTCTG ATGGGGATCC TCTCTTTTCA AATCCGACAA 
TTCAAGCGCC TTTATGAGCA CATCAAGAAT GACAAGTACC TGGTTGGACA GAGACTAGTG 
AACTATGAAC GGAAAGCTGG CAAAGCCAGT GCCAGCACAC ACAGCAGTTC AGTTCAGGAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium.
Briefings in bioinformatics12(5)449-62(2011 Sep)
Gaudet P,Livstone MS,Lewis SE,Thomas PD

Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Proceedings of the National Academy of Sciences of the United States of America99(26)16899-903(2002 Dec)
Strausberg RL,Feingold EA,Grouse LH,Derge JG,Klausner RD,Collins FS,Wagner L,Shenmen CM,Schuler GD,Altschul SF,Zeeberg B,Buetow KH,Schaefer CF,Bhat NK,Hopkins RF,Jordan H,Moore T,Max SI,Wang J,Hsieh F,Diatchenko L,Marusina K,Farmer AA,Rubin GM,Hong L,Stapleton M,Soares MB,Bonaldo MF,Casavant TL,Scheetz TE,Brownstein MJ,Usdin TB,Toshiyuki S,Carninci P,Prange C,Raha SS,Loquellano NA,Peters GJ,Abramson RD,Mullahy SJ,Bosak SA,McEwan PJ,McKernan KJ,Malek JA,Gunaratne PH,Richards S,Worley KC,Hale S,Garcia AM,Gay LJ,Hulyk SW,Villalon DK,Muzny DM,Sodergren EJ,Lu X,Gibbs RA,Fahey J,Helton E,Ketteman M,Madan A,Rodrigues S,Sanchez A,Whiting M,Madan A,Young AC,Shevchenko Y,Bouffard GG,Blakesley RW,Touchman JW,Green ED,Dickson MC,Rodriguez AC,Grimwood J,Schmutz J,Myers RM,Butterfield YS,Krzywinski MI,Skalska U,Smailus DE,Schnerch A,Schein JE,Jones SJ,Marra MA,

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march6 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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