plcb3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	plcb3
Entrez Gene ID	100001532
Full Name	phospholipase C, beta 3 (phosphatidylinositol-specific)
Synonyms	Plcbeta3,she,si:ch211-188c22.1,si:zc188c22.1,wu:fl04b08
General protein information	Preferred Names phospholipase C, beta 3 (phosphatidylinositol-specific) Names 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 schmerle
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005166533.4	XP_005166590.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 isoform X1
XM_005166533.3	XP_005166590.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 isoform X1
NM_001122773.1	NP_001116245.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	plcb3	NP_001116245.1
Pan troglodytes (chimpanzee)	PLCB3	XP_003313091.1
	PLCB3	XP_001115104.2
Bos taurus (cattle)	PLCB3	NP_001179352.1
Rattus norvegicus (Norway rat)	Plcb3	NP_203501.1
Xenopus tropicalis (tropical clawed frog)	plcb3	XP_002940270.2
Canis lupus familiaris (dog)	PLCB3	XP_005631608.1
Mus musculus (house mouse)	Plcb3	NP_032900.2
Homo sapiens (human)	PLCB3	NP_000923.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Silencing of odorant receptor genes by G protein ?? signaling ensures the expression of one odorant receptor per olfactory sensory neuron.
Ferreira T, Wilson SR, Choi YG, Risso D, Dudoit S, Speed TP, Ngai J
Neuron81(4)847-59(2014 Feb)

Revealing the role of phospholipase C?3 in the regulation of VEGF-induced vascular permeability.
Hoeppner LH, Phoenix KN, Clark KJ, Bhattacharya R, Gong X, Sciuto TE, Vohra P, Suresh S, Bhattacharya S, Dvorak AM, Ekker SC, Dvorak HF, Claffey KP, Mukhopadhyay D
Blood120(11)2167-73(2012 Sep)

Craniofacial skeletal defects of adult zebrafish Glypican 4 (knypek) mutants.
LeClair EE, Mui SR, Huang A, Topczewska JM, Topczewski J
Developmental dynamics : an official publication of the American Association of Anatomists238(10)2550-63(2009 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Schmerle (she) encodes a zebrafish ortholog of Phospholipase C, beta 3 (Plcbeta3) which is required in cranial neural crest cells for Edn1 regulation of pharyngeal arch patterning.
Title: phospholipase C, beta 3 is required for Endothelin1 regulation of pharyngeal arch patterning in zebrafish.

The following plcb3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the plcb3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa34349	NM_001122773.1 Latest version!	Danio rerio phospholipase C, beta 3 (phosphatidylinositol-specific) (plcb3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa13543	XM_005166533.4 Latest version!	Danio rerio phospholipase C, beta 3 (phosphatidylinositol-specific) (plcb3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
ODa13543	XM_005166533.3	Danio rerio phospholipase C, beta 3 (phosphatidylinositol-specific) (plcb3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

plcb3 ( NM_001122773.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001116245.1 Danio rerio phospholipase C, beta 3 (phosphatidylinositol-specific) (plcb3), mRNA.

Price & Availability↑

CloneID	ODa34349
Clone ID Related Accession (Same CDS sequence)	NM_001122773.1
Accession Version	NM_001122773.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EF204528.1. On Dec 22, 2008 this sequence version replaced XM_695678.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF204528.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCCGGGG CGAAACCGGG AGTTCACGCG TTGCAGCTCA AACCGGTGTC TGTCAATGAG 
GTCCTCAAAC GGGGGAGCAA GTTTATCAAA TGGGACGAGG AGGCCAACAG CACACCCAGC 
CTGATCACCC TCAGAGTGGA CCCAAATGGA TTCTTTCTTT ACTGGACTGG AGGAACCAGC 
ATGGAAGCGG AAATCCTGGA CATGAGTCAT ATCAGGGACA CCAGGACAGG GAAGTTTGCT 
AAGACGCCAA AGGACCAACG TATTCGTGAA ATGTTGTTTT CGGGCAAAGC AGATGGGAAC 
TCTGACTCAA GGCTGCTAAC GGTCGTCTAC GGCAACGACC TGGTCAACAT CTCTTTCCTC 
AACTTCCAAG CTGTGCAGGA TGGCTATTGC AAGTTGTGGA CGGATGAACT GTTTAAACTT 
GCCACAAATA TGCTGTCCCA GAATGCTTCC CGCAACACCT TCTTACTCAA AGCTTACACA 
AAGCTAAAGT TGCAGGTAAC TCAGGATGGG AAAATCCCAG TAAAGAACAT CCTAAAAATG 
TTCTCGGATA AGAAACGTGT GGAGATGGCG CTGGAGCACT GCGGTCTTAT CTTTAACAGG 
GTGGACGGTA TAAAACCAGA TGACTTCACC TGGGAAATGT TCCAGAACTT TTTAAACAAA 
CTAAGTCCCA GACCAGAGGT GGAGCGAATT TTTGTAGATC TAGGGTCTAA AGGGAAGCCC 
TTCTTGTCCT TGGATCAGTT GATGGACTTC ATAAATCGCA AACAGAGGGA TTCACGACTA 
AATGAGGTTC TTTACCCTCC ACTCAAACGA GATCAGGTCC GCCAGCTCAT GGAGAAATAT 
GAGACCAACA CCAGCCAGCT GGAGAGAGAT CAGATCTCCC TGATGAGCTT CACAAAGTAT 
CTTGGCGGAG AGGAAAACAC TGTGGTTCCC CCTGAGAGGC TGGACATTAT CGATGATATG 
AACCAGCCGC TGTCCCACTA TTTCATCAAC TCATCACACA ACACATACCT TACGGTGGGG 
CAGCTGACAG GCCTGTCTTC AGTTGAGATG TACAGACAGG TGTTGCTGAC AGGATGCCGC 
TGTATAGAGC TGGATTGTTG GAAGGGCCGC CCACCTGAAG AAGAGCCAAT CATCACTCAT 
GGTTTCACCA TGACAACTGA GATCCCATTC AAGGATGTGA TTGAAGCCAT TGCTGAAAGT 
GCTTTCAAGA CGTCACCTTA CCCTCTCATC CTGTCCTTTG AGAACCACGT GGACTCAGCA 
AAGCAGCAGG CTAAAATGGC TGAATATTGT CGCTCAATGT TTGGGGATGC GCTGCTCATT 
GACCCACTGG ACAAATATCC GCTGGTTCCT GGTCAACAAC TCCCAAGCCC CCAGGAGCTG 
TTGGGGAAGA TCTTGATCAA GAACAAGAAG AAACATCACC ACAGGGCCTC TAACGGTGGG 
AGCATCCGAC GCAAGGATGG GACAGATGAA CAGTCTTCAC CTTTGAATGA TTGTCCATTG 
TCTGATGGGG ATGTGGGCCA GCTCATGTCC AATGGGGGTG AGAAGCTTGC AGAGAGGATG 
GCCAAAGACC ATGATAACAG CAAATCTATT GATCGGGGAG ATGGTGAGAG TGATGAGGAG 
GAGGAAGAGG AACCCATTCC AGATCCAAAG AAACACAACA ACACTGATGA GGGTACTGCA 
TATAGTGAGG TGAATGCCAC AGAGGAGATG TCCACATTGG TCAACTACAT AGAACCAGTC 
AAATTCAAGT CTTTTGAGGT GGCAGCCAAG AGGAAAAAGT TCTTTGAGAT GTCATCTTTT 
GTGGAGACCA AAGGCATGGA CACATTGAAA AATTCTCCCA CCGAGTTTGT TGAATATAAT 
AAAAAGCAAA TGAGTCGCAT TTACCCCAAA GGGACTCGTG TGGACTCCAG CAACTATATG 
CCACAGCTTT TCTGGAACGT GGGCTGCCAG ATGGTGGCAC TAAACTTCCA AACCCTTGAC 
CTGGCGATGC AGCTGAACAT GGGGGTGTTT GAATACAATG GCCGCTGTGG CTATCTGCTG 
AAACCGGAGT TCATGAGACG CACTGACAAA CACTTTGACC CCTTTACTAC AGACATTGTG 
GATGGCATTG TAGCCAATAC AGTTAAAGTC AAGATCATCT CAGGCCAGTT TCTAAATGAT 
AAGAAGGTTG GTGTTTATGT GGAGGTGGAC ATGTTTGGGC TTCCTGCAGA CACTAAAAGG 
AAATATCGAA CAAAAACTTC AAACAACAAC TCTCTGGACC CAGTCTGGGA TGATGAAACC 
TTTGTGTTCA ATAAGGTGGT GTTACCTACG CTAGCATCTC TGAGAGTGGC GGTATATGAA 
GAAAACGGAA AGTTTATTGG TCATCGTATC TTGCCAGTGT CAGCGTTGCG CCCAGGATAT 
CACTACATCT GTCTAAAGAA TGAACTGAAC CAACCACTCA TGCTGCCTTC CCTGCTTGTG 
TACACTGAGG TACAGGACTA CATTCCCAAC GAGCACCAAG AATATGCAGA GGCTCTAACA 
AACCCCATCA AGCATCTCTC TCTGCTGGCC CAGAGAGAAA GTCAGCTATT AGCACTTATG 
GAGGATCCCA CTGAGGGTGG GCCTACAAAA CAGGAGGAAG GAGACGGGAC TGACTCTAGC 
TGCGTGACCT CTGCTGACCC TATCTTCCCT CTGCCCTCAT CTCCAACACT TCCTAGTGTA 
CCAACTGACT TGAACCCTCC TCCATCAGGC CAAAAAGAAG ATCTAGTCAA CAATGTCCTT 
AAAGTAATCG AGCCTGAGTC TATTGATGAG CTTAAACAAC ACAATTCCTA CCTGAAGCTG 
CTGAAGAAGC AATGCAAGGA ATTTAAGGAG CTGAGCAAGA AACATCTCAA AAAGATGTTG 
GCCCTCAGTA AGGAACAAAA GACTCTCTTT AATCAGCAGA GCTCGGAACT ACAGAGGAAA 
CGCAGTCAGA TTGAGAAGCG TATACGCACA AGTCTAAAGA AAAATGGATC AGAAGATTCT 
GTGCATAGGG AATTAAATGA GCTGGATCTG GAGCTACAGA AACAAATGGA GCAGTTCAGA 
GAGGAGCACA TGCAAAAGCT TCTTGTACTC AGACAAGGAC AGCTTCAGAT GGAGAAAGAA 
GTGAAACAGT TCCACCTCAA AGAGGCCAAT CAGAAACTGA AGGATATTGC CATCGAATGC 
CAAGCAGCCC AGATCAAGAA ACTCAAAGAT GTGTGTGACA AAGAGAAAAA GGAGTTGCAG 
AAGATGCTGG ATAGAAAGAG GCTAAACAGC ATAGTTGAGG CCAGAAAAGC AGAAAAGGAG 
ATAGATGTGA ACGAGATCAA TAGAAAACAT ATCCAAGAGT CGGTCAGCTT AATCCGTCAG 
CTGGAAGTAT CTCAGGGGAA GCGTCAGGAG AAGTTGAAAG CCCTTCATAA GGACATCCTT 
CAGAAAATCG AGGAGGAAAT TCCTATGAAT CAGGCCCGTT TGACCCGTGA CTTTGAGGCA 
GAGCTAAAGC GTTTGCCAGA GGAGATTTCT CAATATCTGC AGGAGCTTGA GAGCAAAGGC 
TCTCGGAAAG ACAGCTTGAT GGGTCAGACC AATGACGGCC CAAACTCTGG CCCCCCATCC 
AACTGCAGCA CACCACCTTA CTCCTCCCCC AACCACAGCT GGAGCAACAT AGCCAATGTT 
TCATCCTTAC TGCTGGACTC CTCCACCTCA AGCACTTCTG CCAAGTCCGA GACAGACACT 
ACAGGAGTCT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001116245.1
CDS	22..3756
Translation	MAGAKPGVHALQLKPVSVNEVLKRGSKFIKWDEEANSTPSLITLRVDPNGFFLYWTGGTSMEAEILDMSHIRDTRTGKFAKTPKDQRIREMLFSGKADGNSDSRLLTVVYGNDLVNISFLNFQAVQDGYCKLWTDELFKLATNMLSQNASRNTFLLKAYTKLKLQVTQDGKIPVKNILKMFSDKKRVEMALEHCGLIFNRVDGIKPDDFTWEMFQNFLNKLSPRPEVERIFVDLGSKGKPFLSLDQLMDFINRKQRDSRLNEVLYPPLKRDQVRQLMEKYETNTSQLERDQISLMSFTKYLGGEENTVVPPERLDIIDDMNQPLSHYFINSSHNTYLTVGQLTGLSSVEMYRQVLLTGCRCIELDCWKGRPPEEEPIITHGFTMTTEIPFKDVIEAIAESAFKTSPYPLILSFENHVDSAKQQAKMAEYCRSMFGDALLIDPLDKYPLVPGQQLPSPQELLGKILIKNKKKHHHRASNGGSIRRKDGTDEQSSPLNDCPLSDGDVGQLMSNGGEKLAERMAKDHDNSKSIDRGDGESDEEEEEEPIPDPKKHNNTDEGTAYSEVNATEEMSTLVNYIEPVKFKSFEVAAKRKKFFEMSSFVETKGMDTLKNSPTEFVEYNKKQMSRIYPKGTRVDSSNYMPQLFWNVGCQMVALNFQTLDLAMQLNMGVFEYNGRCGYLLKPEFMRRTDKHFDPFTTDIVDGIVANTVKVKIISGQFLNDKKVGVYVEVDMFGLPADTKRKYRTKTSNNNSLDPVWDDETFVFNKVVLPTLASLRVAVYEENGKFIGHRILPVSALRPGYHYICLKNELNQPLMLPSLLVYTEVQDYIPNEHQEYAEALTNPIKHLSLLAQRESQLLALMEDPTEGGPTKQEEGDGTDSSCVTSADPIFPLPSSPTLPSVPTDLNPPPSGQKEDLVNNVLKVIEPESIDELKQHNSYLKLLKKQCKEFKELSKKHLKKMLALSKEQKTLFNQQSSELQRKRSQIEKRIRTSLKKNGSEDSVHRELNELDLELQKQMEQFREEHMQKLLVLRQGQLQMEKEVKQFHLKEANQKLKDIAIECQAAQIKKLKDVCDKEKKELQKMLDRKRLNSIVEARKAEKEIDVNEINRKHIQESVSLIRQLEVSQGKRQEKLKALHKDILQKIEEEIPMNQARLTRDFEAELKRLPEEISQYLQELESKGSRKDSLMGQTNDGPNSGPPSNCSTPPYSSPNHSWSNIANVSSLLLDSSTSSTSAKSETDTTGVY

Target ORF information:

RefSeq Version	NM_001122773.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phospholipase C, beta 3 (phosphatidylinositol-specific) (plcb3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001122773.1

ORF Insert Sequence:

ATGGCCGGGG CGAAACCGGG AGTTCACGCG TTGCAGCTCA AACCGGTGTC TGTCAATGAG 
GTCCTCAAAC GGGGGAGCAA GTTTATCAAA TGGGACGAGG AGGCCAACAG CACACCCAGC 
CTGATCACCC TCAGAGTGGA CCCAAATGGA TTCTTTCTTT ACTGGACTGG AGGAACCAGC 
ATGGAAGCGG AAATCCTGGA CATGAGTCAT ATCAGGGACA CCAGGACAGG GAAGTTTGCT 
AAGACGCCAA AGGACCAACG TATTCGTGAA ATGTTGTTTT CGGGCAAAGC AGATGGGAAC 
TCTGACTCAA GGCTGCTAAC GGTCGTCTAC GGCAACGACC TGGTCAACAT CTCTTTCCTC 
AACTTCCAAG CTGTGCAGGA TGGCTATTGC AAGTTGTGGA CGGATGAACT GTTTAAACTT 
GCCACAAATA TGCTGTCCCA GAATGCTTCC CGCAACACCT TCTTACTCAA AGCTTACACA 
AAGCTAAAGT TGCAGGTAAC TCAGGATGGG AAAATCCCAG TAAAGAACAT CCTAAAAATG 
TTCTCGGATA AGAAACGTGT GGAGATGGCG CTGGAGCACT GCGGTCTTAT CTTTAACAGG 
GTGGACGGTA TAAAACCAGA TGACTTCACC TGGGAAATGT TCCAGAACTT TTTAAACAAA 
CTAAGTCCCA GACCAGAGGT GGAGCGAATT TTTGTAGATC TAGGGTCTAA AGGGAAGCCC 
TTCTTGTCCT TGGATCAGTT GATGGACTTC ATAAATCGCA AACAGAGGGA TTCACGACTA 
AATGAGGTTC TTTACCCTCC ACTCAAACGA GATCAGGTCC GCCAGCTCAT GGAGAAATAT 
GAGACCAACA CCAGCCAGCT GGAGAGAGAT CAGATCTCCC TGATGAGCTT CACAAAGTAT 
CTTGGCGGAG AGGAAAACAC TGTGGTTCCC CCTGAGAGGC TGGACATTAT CGATGATATG 
AACCAGCCGC TGTCCCACTA TTTCATCAAC TCATCACACA ACACATACCT TACGGTGGGG 
CAGCTGACAG GCCTGTCTTC AGTTGAGATG TACAGACAGG TGTTGCTGAC AGGATGCCGC 
TGTATAGAGC TGGATTGTTG GAAGGGCCGC CCACCTGAAG AAGAGCCAAT CATCACTCAT 
GGTTTCACCA TGACAACTGA GATCCCATTC AAGGATGTGA TTGAAGCCAT TGCTGAAAGT 
GCTTTCAAGA CGTCACCTTA CCCTCTCATC CTGTCCTTTG AGAACCACGT GGACTCAGCA 
AAGCAGCAGG CTAAAATGGC TGAATATTGT CGCTCAATGT TTGGGGATGC GCTGCTCATT 
GACCCACTGG ACAAATATCC GCTGGTTCCT GGTCAACAAC TCCCAAGCCC CCAGGAGCTG 
TTGGGGAAGA TCTTGATCAA GAACAAGAAG AAACATCACC ACAGGGCCTC TAACGGTGGG 
AGCATCCGAC GCAAGGATGG GACAGATGAA CAGTCTTCAC CTTTGAATGA TTGTCCATTG 
TCTGATGGGG ATGTGGGCCA GCTCATGTCC AATGGGGGTG AGAAGCTTGC AGAGAGGATG 
GCCAAAGACC ATGATAACAG CAAATCTATT GATCGGGGAG ATGGTGAGAG TGATGAGGAG 
GAGGAAGAGG AACCCATTCC AGATCCAAAG AAACACAACA ACACTGATGA GGGTACTGCA 
TATAGTGAGG TGAATGCCAC AGAGGAGATG TCCACATTGG TCAACTACAT AGAACCAGTC 
AAATTCAAGT CTTTTGAGGT GGCAGCCAAG AGGAAAAAGT TCTTTGAGAT GTCATCTTTT 
GTGGAGACCA AAGGCATGGA CACATTGAAA AATTCTCCCA CCGAGTTTGT TGAATATAAT 
AAAAAGCAAA TGAGTCGCAT TTACCCCAAA GGGACTCGTG TGGACTCCAG CAACTATATG 
CCACAGCTTT TCTGGAACGT GGGCTGCCAG ATGGTGGCAC TAAACTTCCA AACCCTTGAC 
CTGGCGATGC AGCTGAACAT GGGGGTGTTT GAATACAATG GCCGCTGTGG CTATCTGCTG 
AAACCGGAGT TCATGAGACG CACTGACAAA CACTTTGACC CCTTTACTAC AGACATTGTG 
GATGGCATTG TAGCCAATAC AGTTAAAGTC AAGATCATCT CAGGCCAGTT TCTAAATGAT 
AAGAAGGTTG GTGTTTATGT GGAGGTGGAC ATGTTTGGGC TTCCTGCAGA CACTAAAAGG 
AAATATCGAA CAAAAACTTC AAACAACAAC TCTCTGGACC CAGTCTGGGA TGATGAAACC 
TTTGTGTTCA ATAAGGTGGT GTTACCTACG CTAGCATCTC TGAGAGTGGC GGTATATGAA 
GAAAACGGAA AGTTTATTGG TCATCGTATC TTGCCAGTGT CAGCGTTGCG CCCAGGATAT 
CACTACATCT GTCTAAAGAA TGAACTGAAC CAACCACTCA TGCTGCCTTC CCTGCTTGTG 
TACACTGAGG TACAGGACTA CATTCCCAAC GAGCACCAAG AATATGCAGA GGCTCTAACA 
AACCCCATCA AGCATCTCTC TCTGCTGGCC CAGAGAGAAA GTCAGCTATT AGCACTTATG 
GAGGATCCCA CTGAGGGTGG GCCTACAAAA CAGGAGGAAG GAGACGGGAC TGACTCTAGC 
TGCGTGACCT CTGCTGACCC TATCTTCCCT CTGCCCTCAT CTCCAACACT TCCTAGTGTA 
CCAACTGACT TGAACCCTCC TCCATCAGGC CAAAAAGAAG ATCTAGTCAA CAATGTCCTT 
AAAGTAATCG AGCCTGAGTC TATTGATGAG CTTAAACAAC ACAATTCCTA CCTGAAGCTG 
CTGAAGAAGC AATGCAAGGA ATTTAAGGAG CTGAGCAAGA AACATCTCAA AAAGATGTTG 
GCCCTCAGTA AGGAACAAAA GACTCTCTTT AATCAGCAGA GCTCGGAACT ACAGAGGAAA 
CGCAGTCAGA TTGAGAAGCG TATACGCACA AGTCTAAAGA AAAATGGATC AGAAGATTCT 
GTGCATAGGG AATTAAATGA GCTGGATCTG GAGCTACAGA AACAAATGGA GCAGTTCAGA 
GAGGAGCACA TGCAAAAGCT TCTTGTACTC AGACAAGGAC AGCTTCAGAT GGAGAAAGAA 
GTGAAACAGT TCCACCTCAA AGAGGCCAAT CAGAAACTGA AGGATATTGC CATCGAATGC 
CAAGCAGCCC AGATCAAGAA ACTCAAAGAT GTGTGTGACA AAGAGAAAAA GGAGTTGCAG 
AAGATGCTGG ATAGAAAGAG GCTAAACAGC ATAGTTGAGG CCAGAAAAGC AGAAAAGGAG 
ATAGATGTGA ACGAGATCAA TAGAAAACAT ATCCAAGAGT CGGTCAGCTT AATCCGTCAG 
CTGGAAGTAT CTCAGGGGAA GCGTCAGGAG AAGTTGAAAG CCCTTCATAA GGACATCCTT 
CAGAAAATCG AGGAGGAAAT TCCTATGAAT CAGGCCCGTT TGACCCGTGA CTTTGAGGCA 
GAGCTAAAGC GTTTGCCAGA GGAGATTTCT CAATATCTGC AGGAGCTTGA GAGCAAAGGC 
TCTCGGAAAG ACAGCTTGAT GGGTCAGACC AATGACGGCC CAAACTCTGG CCCCCCATCC 
AACTGCAGCA CACCACCTTA CTCCTCCCCC AACCACAGCT GGAGCAACAT AGCCAATGTT 
TCATCCTTAC TGCTGGACTC CTCCACCTCA AGCACTTCTG CCAAGTCCGA GACAGACACT 
ACAGGAGTCT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plcb3 ( XM_005166533.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166590.1 Danio rerio phospholipase C, beta 3 (phosphatidylinositol-specific) (plcb3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa13543
Clone ID Related Accession (Same CDS sequence)	XM_005166533.4 , XM_005166533.3
Accession Version	XM_005166533.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3738bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005166533.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CGAAACCGGG AGTTCACGCG TTGCAGCTCA AACCGGTGTC TGTCAATGAG 
GTCCTCAAAC GGGGGAGCAA GTTTATCAAA TGGGACGAGG AGGCCAACAG CACACCCAGC 
CTGATCACCC TCAGAGTGGA CCCAAATGGA TTCTTTCTTT ACTGGACTGG AGGAACCAGC 
ATGGAAGCGG AAATCCTGGA CATGAGTCAT ATCAGGGACA CCAGGACAGG GAAGTTTGCT 
AAGACGCCAA AGGACCAACG TATTCGTGAA ATGTTGTTTT CGGGCAAAGC AGATGGGAAC 
TCTGACTCAA GGCTGCTAAC GGTCGTCTAC GGCAACGACC TGGTCAACAT CTCTTTCCTC 
AACTTCCAAG CTGTGCAGGA TGGCTATTGC AAGTTGTGGA CGGATGAACT GTTTAAACTT 
GCCACAAATA TGCTGTCCCA GAATGCTTCC CGCAACACCT TCTTACTCAA AGCTTACACA 
AAGCTAAAGT TGCAGGTAAC TCAGGATGGG AAAATCCCAG TAAAGAACAT CCTAAAAATG 
TTCTCGGATA AGAAACGTGT GGAGATGGCG CTGGAGCACT GCGGTCTTAT CTTTAACAGG 
GTGGACGGTA TAAAACCAGA TGACTTCACC TGGGAAATGT TCCAGAACTT TTTAAACAAA 
CTAAGTCCCA GACCAGAGGT GGAGCGAATT TTTGTAGATC TAGGGTCTAA AGGGAAGCCC 
TTCTTGTCCT TGGATCAGTT GATGGACTTC ATAAATCGCA AACAGAGGGA TTCACGACTA 
AATGAGGTTC TTTACCCTCC ACTCAAACGA GATCAGGTCC GCCAGCTCAT GGAGAAATAT 
GAGACCAACA CCAGCCAGCT GGAGAGAGAT CAGATCTCCC TGATGAGCTT CACAAAGTAT 
CTTGGCGGAG AGGAAAACAC TGTGGTTCCC CCTGAGAGGC TGGACATTAT CGATGATATG 
AACCAGCCGC TGTCCCACTA TTTCATCAAC TCATCACACA ACACATACCT TACGGTGGGG 
CAGCTGACAG GCCTGTCTTC AGTTGAGATG TACAGACAGG TGTTGCTGAC AGGATGCCGC 
TGTATAGAGC TGGATTGTTG GAAGGGCCGC CCACCTGAAG AAGAGCCAAT CATCACTCAT 
GGTTTCACCA TGACAACTGA GATCCCATTC AAGGATGTGA TTGAAGCCAT TGCTGAAAGT 
GCTTTCAAGA CGTCACCTTA CCCTCTCATC CTGTCCTTTG AGAACCACGT GGACTCAGCA 
AAGCAGCAGG CTAAAATGGC TGAATATTGT CGCTCAATGT TTGGGGATGC GCTGCTCATT 
GACCCACTGG ACAAATATCC GCTGGTTCCT GGTCAACAAC TCCCAAGCCC CCAGGAGCTG 
TTGGGGAAGA TCTTGATCAA GAACAAGAAG AAACATCACC ACAGGGCCTC TAACGGTGGG 
AGCATCCGAC GCAAGGATGG GACAGATGAA CAGTCTTCAC CTTTGAATGA TTGTCCATTG 
TCTGATGGGG ATGTGGGCCA GCTCATGTCC AATGGGGGTG AGAAGCTTGC AGAGAGGATG 
GCCAAAGACC ATGATAACAG CAAATCTATT GATCGGGGAG ATGGTGAGAG TGATGAGGAG 
GAGGAAGAGG AACCCATTCC AGATCCAAAG AAACACAACA ACACTGATGA GGGTACTGCA 
TATAGTGAGG TGAATGCCAC AGAGGAGATG TCCACATTGG TCAACTACAT AGAACCAGTC 
AAATTCAAGT CTTTTGAGGT GGCAGCCAAG AGGAAAAAGT TCTTTGAGAT GTCATCTTTT 
GTGGAGACCA AAGGCATGGA CACATTGAAA AATTCTCCCA CCGAGTTTGT TGAATATAAT 
AAAAAGCAAA TGAGTCGCAT TTACCCCAAA GGGACTCGTG TGGACTCCAG CAACTATATG 
CCACAGCTTT TCTGGAACGT GGGCTGCCAG ATGGTGGCAC TAAACTTCCA AACCCTTGAC 
CTGGCGATGC AGCTGAACAT GGGGGTGTTT GAATACAATG GCCGCTGTGG CTATCTGCTG 
AAACCGGAGT TCATGAGACG CACTGACAAA CACTTTGACC CCTTTACTAC AGACATTGTG 
GATGGCATTG TAGCCAATAC AGTTAAAGTC AAGATCATCT CAGGCCAGTT TCTAAATGAT 
AAGAAGGTTG GTGTTTATGT GGAGGTGGAC ATGTTTGGGC TTCCTGCAGA CACTAAAAGG 
AAATATCGAA CAAAAACTTC AAACAACAAC TCTCTGGACC CAGTCTGGGA TGATGAAACC 
TTTGTGTTCA ATAAGGTGGT GTTACCTACG CTAGCATCTC TGAGAGTGGC GGTATATGAA 
GAAAACGGAA AGTTTATTGG TCATCGTATC TTGCCAGTGT CAGCGTTGCG CCCAGGATAT 
CACTACATCT GTCTAAAGAA TGAACTGAAC CAACCACTCA TGCTGCCTTC CCTGCTTGTG 
TACACTGAGG TACAGGACTA CATTCCCAAC GAGCACCAAG AATATGCAGA GGCTCTAACA 
AACCCCATCA AGCATCTCTC TCTGCTGGCC CAGAGAGAAA GTCAGCTATT AGCACTTATG 
GAGGATCCCA CTGAGGGTGG GCCTACAAAA CAGGAGGAAG GAGACGGGAC TGACTCTAGC 
TGCGTGACCT CTGCTGACCC TATCTTCCCT CTGCCCTCAT CTCCAACACT TCCTAGTGTA 
CCAACTGACT TGAACCCTCC TCCATCAGGC CAAAAAGAAG ATCTAGTCAA CAATGTCCTT 
AAAGTAATCG AGCCTGAGTC TGTTGATGAG CTTAAACAAC ACAATTCCTA CCTGAAGCTG 
CTGAAGAAGC AATGCAAGGA ATTTAAGGAG CTGAGCAAGA AACATCTCAA AAAGATGTTG 
GCCCTCAGTA AGGAACAAAA GACTCTCTTT AATCAGCAGA GCTCGGAACT ACAGAGGAAA 
CGCAGTCAGA TTGAGAAGCG TATACGCACA AGTCTAAAGA AAAATGGATC AGAAGATTCT 
GTGCATAGGG AATTAAATGA GCTGGATCTG GAACTACAGA AACAAATGGA GCAGTTCAGA 
GAGGAGCACA TGCAAAAGCT TCTTGTACTC AGACAAGGAC AGCTTCAGAT GGAGAAAGAA 
GTGAAACAGT TCCACCTCAA AGAGGCCAAT CAGAAACTGA AGGATATTGC CATCGAATGC 
CAAGCAGCCC AGATCAAGAA ACTCAAAGAT GTGTGTGACA AAGAGAAAAA GGAGTTGCAG 
AAGATGCTGG ATAGAAAGAG GCTAAACAGC ATAGTTGAGG CCAGAAAAGC AGAAAAGGAG 
ATCAGAGATG TGAACGAGAT CAATAGAAAA CATATCCAAG AGTCGGTCAG CTTAATCCGT 
CAGCTGGAAG TATCTCAGGG GAAGCGTCAG GAGAAGTTGA AAGCCCTTCA TAAGGACATC 
CTTCAGAAAA TCGAGGAGGA AATTCCTATG AATCAGGCCC GTTTGACCCG TGACTTCGAG 
GCAGAGCTAA AGCGTTTGCC AGAGGAGATT TCTCAATATC TGCAGGAGCT TGAGAGCAAA 
GGCTCTCGGA AAGACAGCTT GATGGGTCAG ACCAATGACG GCCCAAACTC TGGCCCCCCA 
TCCAACTGCA GCACACCACC TTACTCCTCC CCCAACCACA GCTGGAGCAA CATAGCCAAT 
GTTTCATCCT TACTGCTGGA CTCCTCCACC TCAAGCTCTT CAGCCAAGTC CGAGACAGAC 
ACTACAGGAG TCTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166590.1
CDS	1022..4759
Translation	MAGAKPGVHALQLKPVSVNEVLKRGSKFIKWDEEANSTPSLITLRVDPNGFFLYWTGGTSMEAEILDMSHIRDTRTGKFAKTPKDQRIREMLFSGKADGNSDSRLLTVVYGNDLVNISFLNFQAVQDGYCKLWTDELFKLATNMLSQNASRNTFLLKAYTKLKLQVTQDGKIPVKNILKMFSDKKRVEMALEHCGLIFNRVDGIKPDDFTWEMFQNFLNKLSPRPEVERIFVDLGSKGKPFLSLDQLMDFINRKQRDSRLNEVLYPPLKRDQVRQLMEKYETNTSQLERDQISLMSFTKYLGGEENTVVPPERLDIIDDMNQPLSHYFINSSHNTYLTVGQLTGLSSVEMYRQVLLTGCRCIELDCWKGRPPEEEPIITHGFTMTTEIPFKDVIEAIAESAFKTSPYPLILSFENHVDSAKQQAKMAEYCRSMFGDALLIDPLDKYPLVPGQQLPSPQELLGKILIKNKKKHHHRASNGGSIRRKDGTDEQSSPLNDCPLSDGDVGQLMSNGGEKLAERMAKDHDNSKSIDRGDGESDEEEEEEPIPDPKKHNNTDEGTAYSEVNATEEMSTLVNYIEPVKFKSFEVAAKRKKFFEMSSFVETKGMDTLKNSPTEFVEYNKKQMSRIYPKGTRVDSSNYMPQLFWNVGCQMVALNFQTLDLAMQLNMGVFEYNGRCGYLLKPEFMRRTDKHFDPFTTDIVDGIVANTVKVKIISGQFLNDKKVGVYVEVDMFGLPADTKRKYRTKTSNNNSLDPVWDDETFVFNKVVLPTLASLRVAVYEENGKFIGHRILPVSALRPGYHYICLKNELNQPLMLPSLLVYTEVQDYIPNEHQEYAEALTNPIKHLSLLAQRESQLLALMEDPTEGGPTKQEEGDGTDSSCVTSADPIFPLPSSPTLPSVPTDLNPPPSGQKEDLVNNVLKVIEPESVDELKQHNSYLKLLKKQCKEFKELSKKHLKKMLALSKEQKTLFNQQSSELQRKRSQIEKRIRTSLKKNGSEDSVHRELNELDLELQKQMEQFREEHMQKLLVLRQGQLQMEKEVKQFHLKEANQKLKDIAIECQAAQIKKLKDVCDKEKKELQKMLDRKRLNSIVEARKAEKEIRDVNEINRKHIQESVSLIRQLEVSQGKRQEKLKALHKDILQKIEEEIPMNQARLTRDFEAELKRLPEEISQYLQELESKGSRKDSLMGQTNDGPNSGPPSNCSTPPYSSPNHSWSNIANVSSLLLDSSTSSSSAKSETDTTGVY

Target ORF information:

RefSeq Version	XM_005166533.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phospholipase C, beta 3 (phosphatidylinositol-specific) (plcb3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166533.4

ORF Insert Sequence:

ATGGCCGGGG CGAAACCGGG AGTTCACGCG TTGCAGCTCA AACCGGTGTC TGTCAATGAG 
GTCCTCAAAC GGGGGAGCAA GTTTATCAAA TGGGACGAGG AGGCCAACAG CACACCCAGC 
CTGATCACCC TCAGAGTGGA CCCAAATGGA TTCTTTCTTT ACTGGACTGG AGGAACCAGC 
ATGGAAGCGG AAATCCTGGA CATGAGTCAT ATCAGGGACA CCAGGACAGG GAAGTTTGCT 
AAGACGCCAA AGGACCAACG TATTCGTGAA ATGTTGTTTT CGGGCAAAGC AGATGGGAAC 
TCTGACTCAA GGCTGCTAAC GGTCGTCTAC GGCAACGACC TGGTCAACAT CTCTTTCCTC 
AACTTCCAAG CTGTGCAGGA TGGCTATTGC AAGTTGTGGA CGGATGAACT GTTTAAACTT 
GCCACAAATA TGCTGTCCCA GAATGCTTCC CGCAACACCT TCTTACTCAA AGCTTACACA 
AAGCTAAAGT TGCAGGTAAC TCAGGATGGG AAAATCCCAG TAAAGAACAT CCTAAAAATG 
TTCTCGGATA AGAAACGTGT GGAGATGGCG CTGGAGCACT GCGGTCTTAT CTTTAACAGG 
GTGGACGGTA TAAAACCAGA TGACTTCACC TGGGAAATGT TCCAGAACTT TTTAAACAAA 
CTAAGTCCCA GACCAGAGGT GGAGCGAATT TTTGTAGATC TAGGGTCTAA AGGGAAGCCC 
TTCTTGTCCT TGGATCAGTT GATGGACTTC ATAAATCGCA AACAGAGGGA TTCACGACTA 
AATGAGGTTC TTTACCCTCC ACTCAAACGA GATCAGGTCC GCCAGCTCAT GGAGAAATAT 
GAGACCAACA CCAGCCAGCT GGAGAGAGAT CAGATCTCCC TGATGAGCTT CACAAAGTAT 
CTTGGCGGAG AGGAAAACAC TGTGGTTCCC CCTGAGAGGC TGGACATTAT CGATGATATG 
AACCAGCCGC TGTCCCACTA TTTCATCAAC TCATCACACA ACACATACCT TACGGTGGGG 
CAGCTGACAG GCCTGTCTTC AGTTGAGATG TACAGACAGG TGTTGCTGAC AGGATGCCGC 
TGTATAGAGC TGGATTGTTG GAAGGGCCGC CCACCTGAAG AAGAGCCAAT CATCACTCAT 
GGTTTCACCA TGACAACTGA GATCCCATTC AAGGATGTGA TTGAAGCCAT TGCTGAAAGT 
GCTTTCAAGA CGTCACCTTA CCCTCTCATC CTGTCCTTTG AGAACCACGT GGACTCAGCA 
AAGCAGCAGG CTAAAATGGC TGAATATTGT CGCTCAATGT TTGGGGATGC GCTGCTCATT 
GACCCACTGG ACAAATATCC GCTGGTTCCT GGTCAACAAC TCCCAAGCCC CCAGGAGCTG 
TTGGGGAAGA TCTTGATCAA GAACAAGAAG AAACATCACC ACAGGGCCTC TAACGGTGGG 
AGCATCCGAC GCAAGGATGG GACAGATGAA CAGTCTTCAC CTTTGAATGA TTGTCCATTG 
TCTGATGGGG ATGTGGGCCA GCTCATGTCC AATGGGGGTG AGAAGCTTGC AGAGAGGATG 
GCCAAAGACC ATGATAACAG CAAATCTATT GATCGGGGAG ATGGTGAGAG TGATGAGGAG 
GAGGAAGAGG AACCCATTCC AGATCCAAAG AAACACAACA ACACTGATGA GGGTACTGCA 
TATAGTGAGG TGAATGCCAC AGAGGAGATG TCCACATTGG TCAACTACAT AGAACCAGTC 
AAATTCAAGT CTTTTGAGGT GGCAGCCAAG AGGAAAAAGT TCTTTGAGAT GTCATCTTTT 
GTGGAGACCA AAGGCATGGA CACATTGAAA AATTCTCCCA CCGAGTTTGT TGAATATAAT 
AAAAAGCAAA TGAGTCGCAT TTACCCCAAA GGGACTCGTG TGGACTCCAG CAACTATATG 
CCACAGCTTT TCTGGAACGT GGGCTGCCAG ATGGTGGCAC TAAACTTCCA AACCCTTGAC 
CTGGCGATGC AGCTGAACAT GGGGGTGTTT GAATACAATG GCCGCTGTGG CTATCTGCTG 
AAACCGGAGT TCATGAGACG CACTGACAAA CACTTTGACC CCTTTACTAC AGACATTGTG 
GATGGCATTG TAGCCAATAC AGTTAAAGTC AAGATCATCT CAGGCCAGTT TCTAAATGAT 
AAGAAGGTTG GTGTTTATGT GGAGGTGGAC ATGTTTGGGC TTCCTGCAGA CACTAAAAGG 
AAATATCGAA CAAAAACTTC AAACAACAAC TCTCTGGACC CAGTCTGGGA TGATGAAACC 
TTTGTGTTCA ATAAGGTGGT GTTACCTACG CTAGCATCTC TGAGAGTGGC GGTATATGAA 
GAAAACGGAA AGTTTATTGG TCATCGTATC TTGCCAGTGT CAGCGTTGCG CCCAGGATAT 
CACTACATCT GTCTAAAGAA TGAACTGAAC CAACCACTCA TGCTGCCTTC CCTGCTTGTG 
TACACTGAGG TACAGGACTA CATTCCCAAC GAGCACCAAG AATATGCAGA GGCTCTAACA 
AACCCCATCA AGCATCTCTC TCTGCTGGCC CAGAGAGAAA GTCAGCTATT AGCACTTATG 
GAGGATCCCA CTGAGGGTGG GCCTACAAAA CAGGAGGAAG GAGACGGGAC TGACTCTAGC 
TGCGTGACCT CTGCTGACCC TATCTTCCCT CTGCCCTCAT CTCCAACACT TCCTAGTGTA 
CCAACTGACT TGAACCCTCC TCCATCAGGC CAAAAAGAAG ATCTAGTCAA CAATGTCCTT 
AAAGTAATCG AGCCTGAGTC TGTTGATGAG CTTAAACAAC ACAATTCCTA CCTGAAGCTG 
CTGAAGAAGC AATGCAAGGA ATTTAAGGAG CTGAGCAAGA AACATCTCAA AAAGATGTTG 
GCCCTCAGTA AGGAACAAAA GACTCTCTTT AATCAGCAGA GCTCGGAACT ACAGAGGAAA 
CGCAGTCAGA TTGAGAAGCG TATACGCACA AGTCTAAAGA AAAATGGATC AGAAGATTCT 
GTGCATAGGG AATTAAATGA GCTGGATCTG GAACTACAGA AACAAATGGA GCAGTTCAGA 
GAGGAGCACA TGCAAAAGCT TCTTGTACTC AGACAAGGAC AGCTTCAGAT GGAGAAAGAA 
GTGAAACAGT TCCACCTCAA AGAGGCCAAT CAGAAACTGA AGGATATTGC CATCGAATGC 
CAAGCAGCCC AGATCAAGAA ACTCAAAGAT GTGTGTGACA AAGAGAAAAA GGAGTTGCAG 
AAGATGCTGG ATAGAAAGAG GCTAAACAGC ATAGTTGAGG CCAGAAAAGC AGAAAAGGAG 
ATCAGAGATG TGAACGAGAT CAATAGAAAA CATATCCAAG AGTCGGTCAG CTTAATCCGT 
CAGCTGGAAG TATCTCAGGG GAAGCGTCAG GAGAAGTTGA AAGCCCTTCA TAAGGACATC 
CTTCAGAAAA TCGAGGAGGA AATTCCTATG AATCAGGCCC GTTTGACCCG TGACTTCGAG 
GCAGAGCTAA AGCGTTTGCC AGAGGAGATT TCTCAATATC TGCAGGAGCT TGAGAGCAAA 
GGCTCTCGGA AAGACAGCTT GATGGGTCAG ACCAATGACG GCCCAAACTC TGGCCCCCCA 
TCCAACTGCA GCACACCACC TTACTCCTCC CCCAACCACA GCTGGAGCAA CATAGCCAAT 
GTTTCATCCT TACTGCTGGA CTCCTCCACC TCAAGCTCTT CAGCCAAGTC CGAGACAGAC 
ACTACAGGAG TCTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

plcb3 ( XM_005166533.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166590.1 Danio rerio phospholipase C, beta 3 (phosphatidylinositol-specific) (plcb3), transcript variant X1, mRNA.

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CloneID	ODa13543
Clone ID Related Accession (Same CDS sequence)	XM_005166533.4 , XM_005166533.3
Accession Version	XM_005166533.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3738bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005166533.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CGAAACCGGG AGTTCACGCG TTGCAGCTCA AACCGGTGTC TGTCAATGAG 
GTCCTCAAAC GGGGGAGCAA GTTTATCAAA TGGGACGAGG AGGCCAACAG CACACCCAGC 
CTGATCACCC TCAGAGTGGA CCCAAATGGA TTCTTTCTTT ACTGGACTGG AGGAACCAGC 
ATGGAAGCGG AAATCCTGGA CATGAGTCAT ATCAGGGACA CCAGGACAGG GAAGTTTGCT 
AAGACGCCAA AGGACCAACG TATTCGTGAA ATGTTGTTTT CGGGCAAAGC AGATGGGAAC 
TCTGACTCAA GGCTGCTAAC GGTCGTCTAC GGCAACGACC TGGTCAACAT CTCTTTCCTC 
AACTTCCAAG CTGTGCAGGA TGGCTATTGC AAGTTGTGGA CGGATGAACT GTTTAAACTT 
GCCACAAATA TGCTGTCCCA GAATGCTTCC CGCAACACCT TCTTACTCAA AGCTTACACA 
AAGCTAAAGT TGCAGGTAAC TCAGGATGGG AAAATCCCAG TAAAGAACAT CCTAAAAATG 
TTCTCGGATA AGAAACGTGT GGAGATGGCG CTGGAGCACT GCGGTCTTAT CTTTAACAGG 
GTGGACGGTA TAAAACCAGA TGACTTCACC TGGGAAATGT TCCAGAACTT TTTAAACAAA 
CTAAGTCCCA GACCAGAGGT GGAGCGAATT TTTGTAGATC TAGGGTCTAA AGGGAAGCCC 
TTCTTGTCCT TGGATCAGTT GATGGACTTC ATAAATCGCA AACAGAGGGA TTCACGACTA 
AATGAGGTTC TTTACCCTCC ACTCAAACGA GATCAGGTCC GCCAGCTCAT GGAGAAATAT 
GAGACCAACA CCAGCCAGCT GGAGAGAGAT CAGATCTCCC TGATGAGCTT CACAAAGTAT 
CTTGGCGGAG AGGAAAACAC TGTGGTTCCC CCTGAGAGGC TGGACATTAT CGATGATATG 
AACCAGCCGC TGTCCCACTA TTTCATCAAC TCATCACACA ACACATACCT TACGGTGGGG 
CAGCTGACAG GCCTGTCTTC AGTTGAGATG TACAGACAGG TGTTGCTGAC AGGATGCCGC 
TGTATAGAGC TGGATTGTTG GAAGGGCCGC CCACCTGAAG AAGAGCCAAT CATCACTCAT 
GGTTTCACCA TGACAACTGA GATCCCATTC AAGGATGTGA TTGAAGCCAT TGCTGAAAGT 
GCTTTCAAGA CGTCACCTTA CCCTCTCATC CTGTCCTTTG AGAACCACGT GGACTCAGCA 
AAGCAGCAGG CTAAAATGGC TGAATATTGT CGCTCAATGT TTGGGGATGC GCTGCTCATT 
GACCCACTGG ACAAATATCC GCTGGTTCCT GGTCAACAAC TCCCAAGCCC CCAGGAGCTG 
TTGGGGAAGA TCTTGATCAA GAACAAGAAG AAACATCACC ACAGGGCCTC TAACGGTGGG 
AGCATCCGAC GCAAGGATGG GACAGATGAA CAGTCTTCAC CTTTGAATGA TTGTCCATTG 
TCTGATGGGG ATGTGGGCCA GCTCATGTCC AATGGGGGTG AGAAGCTTGC AGAGAGGATG 
GCCAAAGACC ATGATAACAG CAAATCTATT GATCGGGGAG ATGGTGAGAG TGATGAGGAG 
GAGGAAGAGG AACCCATTCC AGATCCAAAG AAACACAACA ACACTGATGA GGGTACTGCA 
TATAGTGAGG TGAATGCCAC AGAGGAGATG TCCACATTGG TCAACTACAT AGAACCAGTC 
AAATTCAAGT CTTTTGAGGT GGCAGCCAAG AGGAAAAAGT TCTTTGAGAT GTCATCTTTT 
GTGGAGACCA AAGGCATGGA CACATTGAAA AATTCTCCCA CCGAGTTTGT TGAATATAAT 
AAAAAGCAAA TGAGTCGCAT TTACCCCAAA GGGACTCGTG TGGACTCCAG CAACTATATG 
CCACAGCTTT TCTGGAACGT GGGCTGCCAG ATGGTGGCAC TAAACTTCCA AACCCTTGAC 
CTGGCGATGC AGCTGAACAT GGGGGTGTTT GAATACAATG GCCGCTGTGG CTATCTGCTG 
AAACCGGAGT TCATGAGACG CACTGACAAA CACTTTGACC CCTTTACTAC AGACATTGTG 
GATGGCATTG TAGCCAATAC AGTTAAAGTC AAGATCATCT CAGGCCAGTT TCTAAATGAT 
AAGAAGGTTG GTGTTTATGT GGAGGTGGAC ATGTTTGGGC TTCCTGCAGA CACTAAAAGG 
AAATATCGAA CAAAAACTTC AAACAACAAC TCTCTGGACC CAGTCTGGGA TGATGAAACC 
TTTGTGTTCA ATAAGGTGGT GTTACCTACG CTAGCATCTC TGAGAGTGGC GGTATATGAA 
GAAAACGGAA AGTTTATTGG TCATCGTATC TTGCCAGTGT CAGCGTTGCG CCCAGGATAT 
CACTACATCT GTCTAAAGAA TGAACTGAAC CAACCACTCA TGCTGCCTTC CCTGCTTGTG 
TACACTGAGG TACAGGACTA CATTCCCAAC GAGCACCAAG AATATGCAGA GGCTCTAACA 
AACCCCATCA AGCATCTCTC TCTGCTGGCC CAGAGAGAAA GTCAGCTATT AGCACTTATG 
GAGGATCCCA CTGAGGGTGG GCCTACAAAA CAGGAGGAAG GAGACGGGAC TGACTCTAGC 
TGCGTGACCT CTGCTGACCC TATCTTCCCT CTGCCCTCAT CTCCAACACT TCCTAGTGTA 
CCAACTGACT TGAACCCTCC TCCATCAGGC CAAAAAGAAG ATCTAGTCAA CAATGTCCTT 
AAAGTAATCG AGCCTGAGTC TGTTGATGAG CTTAAACAAC ACAATTCCTA CCTGAAGCTG 
CTGAAGAAGC AATGCAAGGA ATTTAAGGAG CTGAGCAAGA AACATCTCAA AAAGATGTTG 
GCCCTCAGTA AGGAACAAAA GACTCTCTTT AATCAGCAGA GCTCGGAACT ACAGAGGAAA 
CGCAGTCAGA TTGAGAAGCG TATACGCACA AGTCTAAAGA AAAATGGATC AGAAGATTCT 
GTGCATAGGG AATTAAATGA GCTGGATCTG GAACTACAGA AACAAATGGA GCAGTTCAGA 
GAGGAGCACA TGCAAAAGCT TCTTGTACTC AGACAAGGAC AGCTTCAGAT GGAGAAAGAA 
GTGAAACAGT TCCACCTCAA AGAGGCCAAT CAGAAACTGA AGGATATTGC CATCGAATGC 
CAAGCAGCCC AGATCAAGAA ACTCAAAGAT GTGTGTGACA AAGAGAAAAA GGAGTTGCAG 
AAGATGCTGG ATAGAAAGAG GCTAAACAGC ATAGTTGAGG CCAGAAAAGC AGAAAAGGAG 
ATCAGAGATG TGAACGAGAT CAATAGAAAA CATATCCAAG AGTCGGTCAG CTTAATCCGT 
CAGCTGGAAG TATCTCAGGG GAAGCGTCAG GAGAAGTTGA AAGCCCTTCA TAAGGACATC 
CTTCAGAAAA TCGAGGAGGA AATTCCTATG AATCAGGCCC GTTTGACCCG TGACTTCGAG 
GCAGAGCTAA AGCGTTTGCC AGAGGAGATT TCTCAATATC TGCAGGAGCT TGAGAGCAAA 
GGCTCTCGGA AAGACAGCTT GATGGGTCAG ACCAATGACG GCCCAAACTC TGGCCCCCCA 
TCCAACTGCA GCACACCACC TTACTCCTCC CCCAACCACA GCTGGAGCAA CATAGCCAAT 
GTTTCATCCT TACTGCTGGA CTCCTCCACC TCAAGCTCTT CAGCCAAGTC CGAGACAGAC 
ACTACAGGAG TCTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166590.1
CDS	1022..4759
Translation	MAGAKPGVHALQLKPVSVNEVLKRGSKFIKWDEEANSTPSLITLRVDPNGFFLYWTGGTSMEAEILDMSHIRDTRTGKFAKTPKDQRIREMLFSGKADGNSDSRLLTVVYGNDLVNISFLNFQAVQDGYCKLWTDELFKLATNMLSQNASRNTFLLKAYTKLKLQVTQDGKIPVKNILKMFSDKKRVEMALEHCGLIFNRVDGIKPDDFTWEMFQNFLNKLSPRPEVERIFVDLGSKGKPFLSLDQLMDFINRKQRDSRLNEVLYPPLKRDQVRQLMEKYETNTSQLERDQISLMSFTKYLGGEENTVVPPERLDIIDDMNQPLSHYFINSSHNTYLTVGQLTGLSSVEMYRQVLLTGCRCIELDCWKGRPPEEEPIITHGFTMTTEIPFKDVIEAIAESAFKTSPYPLILSFENHVDSAKQQAKMAEYCRSMFGDALLIDPLDKYPLVPGQQLPSPQELLGKILIKNKKKHHHRASNGGSIRRKDGTDEQSSPLNDCPLSDGDVGQLMSNGGEKLAERMAKDHDNSKSIDRGDGESDEEEEEEPIPDPKKHNNTDEGTAYSEVNATEEMSTLVNYIEPVKFKSFEVAAKRKKFFEMSSFVETKGMDTLKNSPTEFVEYNKKQMSRIYPKGTRVDSSNYMPQLFWNVGCQMVALNFQTLDLAMQLNMGVFEYNGRCGYLLKPEFMRRTDKHFDPFTTDIVDGIVANTVKVKIISGQFLNDKKVGVYVEVDMFGLPADTKRKYRTKTSNNNSLDPVWDDETFVFNKVVLPTLASLRVAVYEENGKFIGHRILPVSALRPGYHYICLKNELNQPLMLPSLLVYTEVQDYIPNEHQEYAEALTNPIKHLSLLAQRESQLLALMEDPTEGGPTKQEEGDGTDSSCVTSADPIFPLPSSPTLPSVPTDLNPPPSGQKEDLVNNVLKVIEPESVDELKQHNSYLKLLKKQCKEFKELSKKHLKKMLALSKEQKTLFNQQSSELQRKRSQIEKRIRTSLKKNGSEDSVHRELNELDLELQKQMEQFREEHMQKLLVLRQGQLQMEKEVKQFHLKEANQKLKDIAIECQAAQIKKLKDVCDKEKKELQKMLDRKRLNSIVEARKAEKEIRDVNEINRKHIQESVSLIRQLEVSQGKRQEKLKALHKDILQKIEEEIPMNQARLTRDFEAELKRLPEEISQYLQELESKGSRKDSLMGQTNDGPNSGPPSNCSTPPYSSPNHSWSNIANVSSLLLDSSTSSSSAKSETDTTGVY

Target ORF information:

RefSeq Version	XM_005166533.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio phospholipase C, beta 3 (phosphatidylinositol-specific) (plcb3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166533.3

ORF Insert Sequence:

ATGGCCGGGG CGAAACCGGG AGTTCACGCG TTGCAGCTCA AACCGGTGTC TGTCAATGAG 
GTCCTCAAAC GGGGGAGCAA GTTTATCAAA TGGGACGAGG AGGCCAACAG CACACCCAGC 
CTGATCACCC TCAGAGTGGA CCCAAATGGA TTCTTTCTTT ACTGGACTGG AGGAACCAGC 
ATGGAAGCGG AAATCCTGGA CATGAGTCAT ATCAGGGACA CCAGGACAGG GAAGTTTGCT 
AAGACGCCAA AGGACCAACG TATTCGTGAA ATGTTGTTTT CGGGCAAAGC AGATGGGAAC 
TCTGACTCAA GGCTGCTAAC GGTCGTCTAC GGCAACGACC TGGTCAACAT CTCTTTCCTC 
AACTTCCAAG CTGTGCAGGA TGGCTATTGC AAGTTGTGGA CGGATGAACT GTTTAAACTT 
GCCACAAATA TGCTGTCCCA GAATGCTTCC CGCAACACCT TCTTACTCAA AGCTTACACA 
AAGCTAAAGT TGCAGGTAAC TCAGGATGGG AAAATCCCAG TAAAGAACAT CCTAAAAATG 
TTCTCGGATA AGAAACGTGT GGAGATGGCG CTGGAGCACT GCGGTCTTAT CTTTAACAGG 
GTGGACGGTA TAAAACCAGA TGACTTCACC TGGGAAATGT TCCAGAACTT TTTAAACAAA 
CTAAGTCCCA GACCAGAGGT GGAGCGAATT TTTGTAGATC TAGGGTCTAA AGGGAAGCCC 
TTCTTGTCCT TGGATCAGTT GATGGACTTC ATAAATCGCA AACAGAGGGA TTCACGACTA 
AATGAGGTTC TTTACCCTCC ACTCAAACGA GATCAGGTCC GCCAGCTCAT GGAGAAATAT 
GAGACCAACA CCAGCCAGCT GGAGAGAGAT CAGATCTCCC TGATGAGCTT CACAAAGTAT 
CTTGGCGGAG AGGAAAACAC TGTGGTTCCC CCTGAGAGGC TGGACATTAT CGATGATATG 
AACCAGCCGC TGTCCCACTA TTTCATCAAC TCATCACACA ACACATACCT TACGGTGGGG 
CAGCTGACAG GCCTGTCTTC AGTTGAGATG TACAGACAGG TGTTGCTGAC AGGATGCCGC 
TGTATAGAGC TGGATTGTTG GAAGGGCCGC CCACCTGAAG AAGAGCCAAT CATCACTCAT 
GGTTTCACCA TGACAACTGA GATCCCATTC AAGGATGTGA TTGAAGCCAT TGCTGAAAGT 
GCTTTCAAGA CGTCACCTTA CCCTCTCATC CTGTCCTTTG AGAACCACGT GGACTCAGCA 
AAGCAGCAGG CTAAAATGGC TGAATATTGT CGCTCAATGT TTGGGGATGC GCTGCTCATT 
GACCCACTGG ACAAATATCC GCTGGTTCCT GGTCAACAAC TCCCAAGCCC CCAGGAGCTG 
TTGGGGAAGA TCTTGATCAA GAACAAGAAG AAACATCACC ACAGGGCCTC TAACGGTGGG 
AGCATCCGAC GCAAGGATGG GACAGATGAA CAGTCTTCAC CTTTGAATGA TTGTCCATTG 
TCTGATGGGG ATGTGGGCCA GCTCATGTCC AATGGGGGTG AGAAGCTTGC AGAGAGGATG 
GCCAAAGACC ATGATAACAG CAAATCTATT GATCGGGGAG ATGGTGAGAG TGATGAGGAG 
GAGGAAGAGG AACCCATTCC AGATCCAAAG AAACACAACA ACACTGATGA GGGTACTGCA 
TATAGTGAGG TGAATGCCAC AGAGGAGATG TCCACATTGG TCAACTACAT AGAACCAGTC 
AAATTCAAGT CTTTTGAGGT GGCAGCCAAG AGGAAAAAGT TCTTTGAGAT GTCATCTTTT 
GTGGAGACCA AAGGCATGGA CACATTGAAA AATTCTCCCA CCGAGTTTGT TGAATATAAT 
AAAAAGCAAA TGAGTCGCAT TTACCCCAAA GGGACTCGTG TGGACTCCAG CAACTATATG 
CCACAGCTTT TCTGGAACGT GGGCTGCCAG ATGGTGGCAC TAAACTTCCA AACCCTTGAC 
CTGGCGATGC AGCTGAACAT GGGGGTGTTT GAATACAATG GCCGCTGTGG CTATCTGCTG 
AAACCGGAGT TCATGAGACG CACTGACAAA CACTTTGACC CCTTTACTAC AGACATTGTG 
GATGGCATTG TAGCCAATAC AGTTAAAGTC AAGATCATCT CAGGCCAGTT TCTAAATGAT 
AAGAAGGTTG GTGTTTATGT GGAGGTGGAC ATGTTTGGGC TTCCTGCAGA CACTAAAAGG 
AAATATCGAA CAAAAACTTC AAACAACAAC TCTCTGGACC CAGTCTGGGA TGATGAAACC 
TTTGTGTTCA ATAAGGTGGT GTTACCTACG CTAGCATCTC TGAGAGTGGC GGTATATGAA 
GAAAACGGAA AGTTTATTGG TCATCGTATC TTGCCAGTGT CAGCGTTGCG CCCAGGATAT 
CACTACATCT GTCTAAAGAA TGAACTGAAC CAACCACTCA TGCTGCCTTC CCTGCTTGTG 
TACACTGAGG TACAGGACTA CATTCCCAAC GAGCACCAAG AATATGCAGA GGCTCTAACA 
AACCCCATCA AGCATCTCTC TCTGCTGGCC CAGAGAGAAA GTCAGCTATT AGCACTTATG 
GAGGATCCCA CTGAGGGTGG GCCTACAAAA CAGGAGGAAG GAGACGGGAC TGACTCTAGC 
TGCGTGACCT CTGCTGACCC TATCTTCCCT CTGCCCTCAT CTCCAACACT TCCTAGTGTA 
CCAACTGACT TGAACCCTCC TCCATCAGGC CAAAAAGAAG ATCTAGTCAA CAATGTCCTT 
AAAGTAATCG AGCCTGAGTC TGTTGATGAG CTTAAACAAC ACAATTCCTA CCTGAAGCTG 
CTGAAGAAGC AATGCAAGGA ATTTAAGGAG CTGAGCAAGA AACATCTCAA AAAGATGTTG 
GCCCTCAGTA AGGAACAAAA GACTCTCTTT AATCAGCAGA GCTCGGAACT ACAGAGGAAA 
CGCAGTCAGA TTGAGAAGCG TATACGCACA AGTCTAAAGA AAAATGGATC AGAAGATTCT 
GTGCATAGGG AATTAAATGA GCTGGATCTG GAACTACAGA AACAAATGGA GCAGTTCAGA 
GAGGAGCACA TGCAAAAGCT TCTTGTACTC AGACAAGGAC AGCTTCAGAT GGAGAAAGAA 
GTGAAACAGT TCCACCTCAA AGAGGCCAAT CAGAAACTGA AGGATATTGC CATCGAATGC 
CAAGCAGCCC AGATCAAGAA ACTCAAAGAT GTGTGTGACA AAGAGAAAAA GGAGTTGCAG 
AAGATGCTGG ATAGAAAGAG GCTAAACAGC ATAGTTGAGG CCAGAAAAGC AGAAAAGGAG 
ATCAGAGATG TGAACGAGAT CAATAGAAAA CATATCCAAG AGTCGGTCAG CTTAATCCGT 
CAGCTGGAAG TATCTCAGGG GAAGCGTCAG GAGAAGTTGA AAGCCCTTCA TAAGGACATC 
CTTCAGAAAA TCGAGGAGGA AATTCCTATG AATCAGGCCC GTTTGACCCG TGACTTCGAG 
GCAGAGCTAA AGCGTTTGCC AGAGGAGATT TCTCAATATC TGCAGGAGCT TGAGAGCAAA 
GGCTCTCGGA AAGACAGCTT GATGGGTCAG ACCAATGACG GCCCAAACTC TGGCCCCCCA 
TCCAACTGCA GCACACCACC TTACTCCTCC CCCAACCACA GCTGGAGCAA CATAGCCAAT 
GTTTCATCCT TACTGCTGGA CTCCTCCACC TCAAGCTCTT CAGCCAAGTC CGAGACAGAC 
ACTACAGGAG TCTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Silencing of odorant receptor genes by G protein ?? signaling ensures the expression of one odorant receptor per olfactory sensory neuron.
Neuron81(4)847-59(2014 Feb)
Ferreira T,Wilson SR,Choi YG,Risso D,Dudoit S,Speed TP,Ngai J

Revealing the role of phospholipase C?3 in the regulation of VEGF-induced vascular permeability.
Blood120(11)2167-73(2012 Sep)
Hoeppner LH,Phoenix KN,Clark KJ,Bhattacharya R,Gong X,Sciuto TE,Vohra P,Suresh S,Bhattacharya S,Dvorak AM,Ekker SC,Dvorak HF,Claffey KP,Mukhopadhyay D

Craniofacial skeletal defects of adult zebrafish Glypican 4 (knypek) mutants.
Developmental dynamics : an official publication of the American Association of Anatomists238(10)2550-63(2009 Oct)
LeClair EE,Mui SR,Huang A,Topczewska JM,Topczewski J

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plcb3 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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