DOCK10 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	DOCK10
Entrez Gene ID	100000949
Full Name	dedicator of cytokinesis 10
General protein information	Preferred Names dedicator of cytokinesis 10 Names dedicator of cytokinesis protein 10 zmp:0000000770
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001341012.8	XP_001341048.5	dedicator of cytokinesis protein 10 isoform X1
XM_021466298.1	XP_021321973.1	dedicator of cytokinesis protein 10 isoform X3
XM_017351136.1	XP_017206625.1	dedicator of cytokinesis protein 10 isoform X2
XM_009291413.2	XP_009289688.1	dedicator of cytokinesis protein 10 isoform X3
XM_009291416.2	XP_009289691.1	dedicator of cytokinesis protein 10 isoform X5
XM_021466297.1	XP_021321972.1	dedicator of cytokinesis protein 10 isoform X2
XM_001341012.7	XP_001341048.5	dedicator of cytokinesis protein 10 isoform X1
XM_009291414.2	XP_009289689.1	dedicator of cytokinesis protein 10 isoform X4

Pathways from BioSystems

Homologies

Bos taurus (cattle)	DOCK10	NP_001178202.2
Xenopus tropicalis (tropical clawed frog)	dock10	XP_002937142.2
Pan troglodytes (chimpanzee)	DOCK10	XP_516126.2
	DOCK10	XP_002799128.1
Gallus gallus (chicken)	DOCK10	XP_001234660.2
Canis lupus familiaris (dog)	DOCK10	XP_005635804.1
Mus musculus (house mouse)	Dock10	NP_780500.2
Rattus norvegicus (Norway rat)	Dock10	XP_006245371.1
Homo sapiens (human)	DOCK10	NP_055504.2
Danio rerio (zebrafish)	dock10	XP_001341048.5

Gene Ontology

The following DOCK10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DOCK10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa47522	XM_009291413.2 Latest version!	Danio rerio dedicator of cytokinesis 10 (dock10), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22-24	$1161.30 $1659.00
ODa47523	XM_009291414.2 Latest version!	Danio rerio dedicator of cytokinesis 10 (dock10), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22-24	$1161.30 $1659.00
ODa47525	XM_009291416.2 Latest version!	Danio rerio dedicator of cytokinesis 10 (dock10), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22-24	$1161.30 $1659.00
ODa149005	XM_021466297.1 Latest version!	Danio rerio dedicator of cytokinesis 10 (dock10), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa149006	XM_021466298.1 Latest version!	Danio rerio dedicator of cytokinesis 10 (dock10), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa55221	XM_017351136.1 Latest version!	Danio rerio dedicator of cytokinesis 10 (dock10), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa20115	XM_001341012.8 Latest version!	Danio rerio dedicator of cytokinesis 10 (dock10), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
View Old Accession Versions >>
ODa20115	XM_001341012.7	Danio rerio dedicator of cytokinesis 10 (dock10), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DOCK10 ( XM_009291413.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009289688.1 Danio rerio dedicator of cytokinesis 10 (dock10), transcript variant X3, mRNA.

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CloneID	ODa47522
Clone ID Related Accession (Same CDS sequence)	XM_009291413.2
Accession Version	XM_009291413.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	dedicator of cytokinesis protein 10 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291413.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG AATCGACCCG GAGATTTACG AAGAGCCTGC TGAAGCCGGG GAGCGCAGCG 
GAGATTCGGC AAACCGCTTC CAGCGCCGTG AGGAACGCTA CTATAACGCA GGAGAAGCCC 
AAACTGATCG ACCCTCTGGA CTATGAAGCT GTGATTTCTG AACTTGAAGC AGAATTCAGA 
GAAGATCCTC TTCAAGATCT CCTGTTATTC CCAGACCTTG ACTTCACTGT GTCCACACTT 
CCTGTAGAAA GGAGAACAGT GAAGTCTACT GTCCCAGAGG GAGCTGATGA ACATGCTGAA 
TGTCTGCTGG TCAGACAGGC CTGCCAATAC TTCAACTCCC AGCTGCACAT TGTTCAGTAT 
AAATATGATG AGTATGGAGG GGACTACCGC ATGCTGCCCA GGAAGCTGTG TAAAGCAGAA 
AAGTTGCCTT CCCACTCTTT TGAGATTGAC AATGAAGATG CGGACAAAGA TGAGGACACA 
ACATCTTTGT CCTCATCTAA AGGAGGGGGC GGCGGTGCAG GCAGTGGAGG CGGAGGAGGA 
ATTGGTGTTT TTAAGTCCGG CTGGCTCTAT AAAGGCAACT TCAACAGCAC CGTCAACAAC 
AGCATCACAG TTCGGTCCTT CAAGCGCCGA TATTTTCAGC TGACTCAGCT CTCTGACAAC 
TCTTACATCA TGAACTTCTA TAAAGATGAG AAAATTTCCA AGGAACCGAA AGGCTGTATT 
TTTCTGGACT CCTGCACCGG GGTGGTTCAG AATAATCGAC TGAGGAAACA TGCGTTTGAG 
CTGAAAATGA ACGAAGTGAC ATATTTTGTC CTGGCTGCCG AGAATGAGCA AGACATGGAC 
GACTGGATCA ACACTTTAAA TCGCATCCTG CAGATCAGCC CTCCAGACGG CCCGGCTCAG 
GACCGCAAGA GTACGGACAT GAGCGACTCT AAAAACGAGA TCAGTGATAT ACCACAAAAT 
AACGTCCCTG TTCATAAAAA CGAAGAAACA ACTGAAAATG AGATCCACCC TGAACTAGCC 
AAGTATATTT CTGAGACAGA AGAAAGCTCG AGAGCTGTCA GAGCAGAGGA GAGACTGAAC 
CTGTTCTCCC TGGACCCTGA TATTCCTGTT TTAAGGAGTC CTAGGACAGA AACCGGGGAG 
TCTTCTTCTG TCCATCCATT TGATGAGAAG CTTGGGAAGA GGCTGATGAT CTGCTGTCGC 
TCACTGAACC TCACACTGCA AGGCTGCGTT AATGAAGCGG ACACCGAACC CACCACTAAT 
ATCGAGCCTT TCTTTGTGTC AGTGTGTCTG CTGGACGTAC GTGAGGGCAG GAAGGTTTCC 
GCTGACTTCC ATGTGGACGT AAACCATGAG TTAGTGCGGC AGATGCTTAA TAGCTCTGGT 
AATCCGGGAG ATCAAGGCTC GGATGTTGGT AATGGTGCAC CGCTGGAAAA TGGACTCACT 
TCCCCGGTAG AAAAGAAACC AGAAGACTGT CAACTAGGCC CAGACATGGA AAAGTGGCTA 
TGCTTTCCCA AACAGGCAAT CTTTTCCATT ACGAACCCGC ACACGGATAT TGTGATGGTG 
GCACGAGTGG AGAAAGTTCT GATGGGAAAT ATTTCCAGTG GTGCAGAGCC CTACATCAAA 
AACACCGATT CCAGCAAGAC TGTACACAAG ATGCTGAAAT CCAATAAGCA GTTCTGTAGC 
AAACTAGGCA AGTACCGAAT GCCTTTCGCC TGGTCTGTAA GGTCAGTGTT TAAGGACAAC 
CAGGGCACAG TGGACAGGGA GTCTCGCTTC TCTCCTCTTT TCAAACAGGA AAGCAACAAG 
ATTTCCACCG AAGACCTCAT CAAGCTCATC ACCGAGTACA GGAGGGCGGA GAAGGCCAGT 
AAACTCCAGA CCATCCCTGG AAACCTTGAA ATAAATGTGG ACTGTGTTCC CATGGAATGT 
CCCAATTGTG TGACGTCCTC ATATGTTGCC CTGAAGCCGT TTGAGGACTG CAGCCAACAT 
GCTCCTACGG TGGAAATCGA TGAGTTCCTG CAGGATTCGT CAAAGTTCGC TCAACCTTAC 
AGAGTCTACA AGAACCACAT CTACATCTAC CCTAAACACC TCAAGTATGA CAGCCAGAAG 
AGCTTTGCTA AGGCTCGAAA TATTGCAGTT TGTGTGGAGT TTCGAAGCTC CGATGAAGAA 
CATGCCAAGC CGCTTAAGTG CATCTATGGA AAGCCCGGAG GCCCAGTTTT TACCACGACT 
GCCTGTACCA CAGTTCTGCA CCATTCACAG AACCCAGACT TTTATGATGA GGTCAAGATT 
GAGTTGCCCA CACATCTTCA TGAGAAACAC CATATACTCT TCTCCTTCTA TCATGTGACC 
TGTGACATCA ATGCCAAGAC CAGCTCTAAG AAGAAAGAGG CTCTTGAATT GCCAGTGGGT 
TATGCATGGC TGCCTCTTCT AAAGGATGGA CGCATTTCCT CTCAGGACTT CAGCATTCCT 
GTCTCCTGCA CTTTGCCTGC TGGCTATTTA CACATTAAAG AGCCTTCGTC CACCAAGAAT 
GGCTCTGATG TGAAGTGGGT CGATGGAGGG AAGCAGATCT TCAAATTGTC CACTTTGGTG 
CTTTCTACAG TGTATACTCA GGATCCCCAT CTAAACCGCT TCTTCCAGCA GTGCCAAAAA 
CGAGAGGCAG AAATCTCTCA ACCTCCTACC TCAAACTTCC TCAACTGCCT TAAAGGGCTC 
TTGAGCATGG AGAAGATTCA TGCCATAATT CGCTTCCTTC CTGTTATCTT CAACCAGCTC 
TTTAAAGTGC TTACGCAAAA CGACTCTGAT GAAGTCACCA CCAGCACCAC CAGGGTCCTG 
ATACACATCT TAGCCAAGTG CCACGAGGAG AACCTGGACC ACTATTTGCA CTCCTACATT 
AAGTTTGTCT TTAAGACTGA AGCGTGTGGC TTCAGAACCA TTCATGAAGA GCTGGCCAAG 
GGAATGACCT CTGACCTGAA GAGCAATGAA CAGTTAGTCA TACGCAACGT CCTGAAGTTC 
TCGTGGTTCT TTCTCGAGAT CATAGTGAAG TCAATGGCTC AGCATCTGCT GGACTCAAAC 
AAGCTCAAGC TGCCGCGACC GCAACGATTT CCTGGCACTT TCCAAAACCG TTTGGAGACG 
TTGATCATGA CCATGTCGGA TCACATTTAC TGGAAGAACA AAGAGCTGGC GGAGGAGACG 
CGGAGTGCAA ATATGGCCAT TGCGTTTTTT GTGAAGCGGT GCTTCACCTT GATGGACAGA 
GGTTTCACCT TTAAACTGAT CAGCAACTAC CTGAACATGA TCACAGCCAC TGACAATAAG 
GCGCTGTGTG AGCTGAAGTT TGAGTTCATA AGGGAAGTGT GTAACTATGA GCACTTTGTC 
CCTTTGAGTC TTCCCATCCC GTCTGCACGA ATCACAGACA ATGCATCAAC AGAAGCCCAA 
AGTACTAAGG TGGACTTCCC AGAGTACAGT TTGACAGAGG ATTTCTGCAG GAAGCATTTT 
CTGTCTGGAT TGTTGTTGCG AGAGCTGGGA CTCGCTTTGC AGGATGAGCA AGATCTGAGA 
CACATCGCTC TGGCCTGCCT GAAGAACCTG ATGGCCAAAC ACTCGCTGGA CGCTCGATAC 
GCCACAAAGG AGAAACAGGC TCGGATAGCT TCTCTTTACC TGCCGCTTTA TGGCCTGATC 
CTGGACAACA TGCCTCGGTT TTTCCTCAGA GATCTGTTTC CAATCTGCCT GACAGCGAGT 
GACCAGGGAT CCAGAGATGA TCTCAGTGTT GGAGTAGGTT TGGGTGGTCA GGTGACCCAT 
GTTTTACGCC ATGGAAACTC AATGGACGCG TCCTTCTCCA AGGAAGTGCT CAACTCCATT 
ACAGCCTTTT CATCATTGGC TGTATCGACC GCTAATCATG CCGACTCCAG GGGTTCTCTG 
ATCAGCATCG AGTCGAACCC CAGCAACAGT GACCGCAACA GTGAGAAGAT GGATGCCTGT 
GAAAAGTTTG CCAGGCCTCA GTCTCTTATT GGCTTTGGAT CCCGCTTTGA TAAACTGGAT 
CAAGCTGAAA CCAGAAGTCT GCTCATGTGT TTCCTGCACA TTATGAAGAC CATCTCAGAG 
GAGGTGATGG TTTCATACTG GCATCGAGCG ATTCACCAGG AGATCAGTGA CTTCTTCAAT 
ATTTTGGAAT TATGCCTTCA GCACTTTCGT TTCCTTGGAA AGCGGCACAT TGCCAGGAAG 
CTAGCGGCCG CAGTTAAGCT GGCTCAGGCC ACACAGAACA ATGGCACCCT GAAGGGCTCT 
AATGTGTCCT ACCACTCTCC AGGGCTCCTA CCCCAATGGA TTCTGTCTGC ACAGGACGGT 
CACAGACACG CTCGCTCTCA GACCATGCCC ATCATCAGGG GGAAAAACGC CCTCACAAAC 
CCCAAACTGC TGCATATGAT GGAGACAGAT GGAAATGTTG CTGAAGGAGA AAACTTTAAC 
CCTACAGACG TGGAGGCGAA CCTTTCTACT GAAGTAGCAC TGACCGTGTT GGATGTTCTG 
GACCTGTTTG TTCGACATCA TAAGAAACAG TTGCAGCACG ATGAAGGACA AAACTCTTTG 
ATGAAGAAAG TGTTCGACAC GTACCTGCTG TTCTTCCAGA TCAACCAGTC CACCACCACC 
CTCAGACACG TGTTCACAGC CCTGCGCCTC TTCATGCAGA AGTTTCCGTC CACGTTTTTC 
CAGGGTAAAG CGGATGTGTG TGGCAGCCTG TGTTACGAGA TCCTGAAGTG CTGTAACCAT 
CGCTCTAGCT CCACACAGAC TGAAGCCGCC GCTCTGCTTT ATTTCCTCAT GAGGAGGAAC 
TTTGAGTTCA CGAAGGGCAA GTGCATCGTC CGCTCACACC TGCAGGTGAT TAAAGCTGTG 
AGTCAGCTGA TCGCGGATGC AGGCATTGGC GGATCACGCT TCCAGCAGTC TCTGGCCATC 
ATCAATAACT TCGCCAATGG GGACACGCCA CTTAAAAACA CCACATTCCC AGCTGACGTA 
AAGGACCTGA CCAAGAGGAT ACGCACCGTC CTGATGGCCA CGTCTCAAAT GAAAGAGCAT 
GAAAAAGACC CGGAAATGCT GGTGGACCTT CAGTACAGTC TGGCCAACTC CTACGCCAGC 
ACACCTGAGC TCAGACGCAC CTGGCTGGAA AGCATGGCCA AAATACACGT CAGAAATGGA 
GATCTGTCTG AGGCAGCCAT GTGCTACATC CACATCTCAG CCCTCATAGC AGAGTCTCTG 
AGGAGAAGAG GTTACTGGAA GTCTGAAAAG GGTCGGATGT CAAGTGTGAG CAAAGAGGGA 
GCCTGTGTAA ATAACTGTAG CCCTTTACTA ACTCCCCAAG ACGGAGAAAC ATCATTCAGT 
ATGGGTTGGG CGGCATTTAC GAATATCTCG CCTAACGTTA AAGAAGAGGG AGCAATGAAA 
GAAGACGCTG GCACTCAGGA TACTCCATAC ACTGAGGACA CGCTGGTGGA GCAGCTGGAG 
ATGTGTGTTG ATTATCTCTG GAAGTCTGAG AGATATGAAC TTATTGCCGA GATCAACAAA 
CCTGTTATTG TCGTCTTTGA GAAGAGAAGA GATTTCAAGC GTCTATCAGA GCTGTACTAT 
GACATTCACC GCTCATACCT GAAGGTGACC GAAGTCGTGA ATTCTGAGAA ACGTCTGTTT 
GGTCGGTACT ATCGCGTGGC TTTCTACGGA CAGGGGTTTT TTGAGGAAGA GGAGAGTAAG 
GAGTTTATCT ACAAGGAGCC CAAACTCACA GGACTGTCCG AAATTTCTCA AAGACTCCTC 
AAACTCTACT CGGACAAGTT TGGAGCTGAT AACGTCAAAA TGATCCAGGA CTCCAACAAG 
GTGAATCCGA AAGACTTGGA CCCCAGATTT GCCTACATCC AGGTCACCTA TGTAGTGCCT 
TATTTTAATG AGAAGGAACA GCTGGAGAAG AAAACCGACT TCGAGCGCCA TCACAACATC 
GACCGATTTG TGTTTGAGAC GCCCTTCACG CTTTCTGGAA AAAAACACGG AGACGTGGAG 
GAACAGTGCA AAAGGAAAAC CATCCTGACC ACGAGCTCTA GTTTCCCTTT CTTGAAGAAG 
CGTATAGAGG TGGTGGAGCA ACAGAGTACC GAGATGAACC CTATTGAGGT GGCGATAGAC 
GAGATGAGCC GAAAAGTGTC TGAACTCAAA CAGCTGTGCA ATATGGAGGA GGTGGACATG 
ATCCGTCTGC AGCTCAAACT ACAGGGCAGC GTCAGCGTCA AGGTAAATGC AGGACCCATG 
GCCTACGCCC GAGCCTTCCT AGAGGAGAAA AATGCTAAGA AATACCCAGA CAACCAGGTC 
AAACTGCTGA AAGAGATCTT CAGACAGTTT GCAGAAGCGT GTGGACAGGC TCTGAGTGTG 
AACGAGCGGC TAATAAAAGA GGATCAGTTT GAGTATCAGA GCGAGATGAA AGCTCACTAT 
AAAGACATGC TCACGGAGCT CTCCGCCATT ATGAATGAGC AGATACCGTA CACGAGACAA 
CCAGGAACAG AGCGACACAG TGCCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289688.1
CDS	144..6653
Translation	MAAESTRRFTKSLLKPGSAAEIRQTASSAVRNATITQEKPKLIDPLDYEAVISELEAEFREDPLQDLLLFPDLDFTVSTLPVERRTVKSTVPEGADEHAECLLVRQACQYFNSQLHIVQYKYDEYGGDYRMLPRKLCKAEKLPSHSFEIDNEDADKDEDTTSLSSSKGGGGGAGSGGGGGIGVFKSGWLYKGNFNSTVNNSITVRSFKRRYFQLTQLSDNSYIMNFYKDEKISKEPKGCIFLDSCTGVVQNNRLRKHAFELKMNEVTYFVLAAENEQDMDDWINTLNRILQISPPDGPAQDRKSTDMSDSKNEISDIPQNNVPVHKNEETTENEIHPELAKYISETEESSRAVRAEERLNLFSLDPDIPVLRSPRTETGESSSVHPFDEKLGKRLMICCRSLNLTLQGCVNEADTEPTTNIEPFFVSVCLLDVREGRKVSADFHVDVNHELVRQMLNSSGNPGDQGSDVGNGAPLENGLTSPVEKKPEDCQLGPDMEKWLCFPKQAIFSITNPHTDIVMVARVEKVLMGNISSGAEPYIKNTDSSKTVHKMLKSNKQFCSKLGKYRMPFAWSVRSVFKDNQGTVDRESRFSPLFKQESNKISTEDLIKLITEYRRAEKASKLQTIPGNLEINVDCVPMECPNCVTSSYVALKPFEDCSQHAPTVEIDEFLQDSSKFAQPYRVYKNHIYIYPKHLKYDSQKSFAKARNIAVCVEFRSSDEEHAKPLKCIYGKPGGPVFTTTACTTVLHHSQNPDFYDEVKIELPTHLHEKHHILFSFYHVTCDINAKTSSKKKEALELPVGYAWLPLLKDGRISSQDFSIPVSCTLPAGYLHIKEPSSTKNGSDVKWVDGGKQIFKLSTLVLSTVYTQDPHLNRFFQQCQKREAEISQPPTSNFLNCLKGLLSMEKIHAIIRFLPVIFNQLFKVLTQNDSDEVTTSTTRVLIHILAKCHEENLDHYLHSYIKFVFKTEACGFRTIHEELAKGMTSDLKSNEQLVIRNVLKFSWFFLEIIVKSMAQHLLDSNKLKLPRPQRFPGTFQNRLETLIMTMSDHIYWKNKELAEETRSANMAIAFFVKRCFTLMDRGFTFKLISNYLNMITATDNKALCELKFEFIREVCNYEHFVPLSLPIPSARITDNASTEAQSTKVDFPEYSLTEDFCRKHFLSGLLLRELGLALQDEQDLRHIALACLKNLMAKHSLDARYATKEKQARIASLYLPLYGLILDNMPRFFLRDLFPICLTASDQGSRDDLSVGVGLGGQVTHVLRHGNSMDASFSKEVLNSITAFSSLAVSTANHADSRGSLISIESNPSNSDRNSEKMDACEKFARPQSLIGFGSRFDKLDQAETRSLLMCFLHIMKTISEEVMVSYWHRAIHQEISDFFNILELCLQHFRFLGKRHIARKLAAAVKLAQATQNNGTLKGSNVSYHSPGLLPQWILSAQDGHRHARSQTMPIIRGKNALTNPKLLHMMETDGNVAEGENFNPTDVEANLSTEVALTVLDVLDLFVRHHKKQLQHDEGQNSLMKKVFDTYLLFFQINQSTTTLRHVFTALRLFMQKFPSTFFQGKADVCGSLCYEILKCCNHRSSSTQTEAAALLYFLMRRNFEFTKGKCIVRSHLQVIKAVSQLIADAGIGGSRFQQSLAIINNFANGDTPLKNTTFPADVKDLTKRIRTVLMATSQMKEHEKDPEMLVDLQYSLANSYASTPELRRTWLESMAKIHVRNGDLSEAAMCYIHISALIAESLRRRGYWKSEKGRMSSVSKEGACVNNCSPLLTPQDGETSFSMGWAAFTNISPNVKEEGAMKEDAGTQDTPYTEDTLVEQLEMCVDYLWKSERYELIAEINKPVIVVFEKRRDFKRLSELYYDIHRSYLKVTEVVNSEKRLFGRYYRVAFYGQGFFEEEESKEFIYKEPKLTGLSEISQRLLKLYSDKFGADNVKMIQDSNKVNPKDLDPRFAYIQVTYVVPYFNEKEQLEKKTDFERHHNIDRFVFETPFTLSGKKHGDVEEQCKRKTILTTSSSFPFLKKRIEVVEQQSTEMNPIEVAIDEMSRKVSELKQLCNMEEVDMIRLQLKLQGSVSVKVNAGPMAYARAFLEEKNAKKYPDNQVKLLKEIFRQFAEACGQALSVNERLIKEDQFEYQSEMKAHYKDMLTELSAIMNEQIPYTRQPGTERHSAI

Target ORF information:

RefSeq Version	XM_009291413.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio dedicator of cytokinesis 10 (dock10), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291413.2

ORF Insert Sequence:

ATGGCAGCGG AATCGACCCG GAGATTTACG AAGAGCCTGC TGAAGCCGGG GAGCGCAGCG 
GAGATTCGGC AAACCGCTTC CAGCGCCGTG AGGAACGCTA CTATAACGCA GGAGAAGCCC 
AAACTGATCG ACCCTCTGGA CTATGAAGCT GTGATTTCTG AACTTGAAGC AGAATTCAGA 
GAAGATCCTC TTCAAGATCT CCTGTTATTC CCAGACCTTG ACTTCACTGT GTCCACACTT 
CCTGTAGAAA GGAGAACAGT GAAGTCTACT GTCCCAGAGG GAGCTGATGA ACATGCTGAA 
TGTCTGCTGG TCAGACAGGC CTGCCAATAC TTCAACTCCC AGCTGCACAT TGTTCAGTAT 
AAATATGATG AGTATGGAGG GGACTACCGC ATGCTGCCCA GGAAGCTGTG TAAAGCAGAA 
AAGTTGCCTT CCCACTCTTT TGAGATTGAC AATGAAGATG CGGACAAAGA TGAGGACACA 
ACATCTTTGT CCTCATCTAA AGGAGGGGGC GGCGGTGCAG GCAGTGGAGG CGGAGGAGGA 
ATTGGTGTTT TTAAGTCCGG CTGGCTCTAT AAAGGCAACT TCAACAGCAC CGTCAACAAC 
AGCATCACAG TTCGGTCCTT CAAGCGCCGA TATTTTCAGC TGACTCAGCT CTCTGACAAC 
TCTTACATCA TGAACTTCTA TAAAGATGAG AAAATTTCCA AGGAACCGAA AGGCTGTATT 
TTTCTGGACT CCTGCACCGG GGTGGTTCAG AATAATCGAC TGAGGAAACA TGCGTTTGAG 
CTGAAAATGA ACGAAGTGAC ATATTTTGTC CTGGCTGCCG AGAATGAGCA AGACATGGAC 
GACTGGATCA ACACTTTAAA TCGCATCCTG CAGATCAGCC CTCCAGACGG CCCGGCTCAG 
GACCGCAAGA GTACGGACAT GAGCGACTCT AAAAACGAGA TCAGTGATAT ACCACAAAAT 
AACGTCCCTG TTCATAAAAA CGAAGAAACA ACTGAAAATG AGATCCACCC TGAACTAGCC 
AAGTATATTT CTGAGACAGA AGAAAGCTCG AGAGCTGTCA GAGCAGAGGA GAGACTGAAC 
CTGTTCTCCC TGGACCCTGA TATTCCTGTT TTAAGGAGTC CTAGGACAGA AACCGGGGAG 
TCTTCTTCTG TCCATCCATT TGATGAGAAG CTTGGGAAGA GGCTGATGAT CTGCTGTCGC 
TCACTGAACC TCACACTGCA AGGCTGCGTT AATGAAGCGG ACACCGAACC CACCACTAAT 
ATCGAGCCTT TCTTTGTGTC AGTGTGTCTG CTGGACGTAC GTGAGGGCAG GAAGGTTTCC 
GCTGACTTCC ATGTGGACGT AAACCATGAG TTAGTGCGGC AGATGCTTAA TAGCTCTGGT 
AATCCGGGAG ATCAAGGCTC GGATGTTGGT AATGGTGCAC CGCTGGAAAA TGGACTCACT 
TCCCCGGTAG AAAAGAAACC AGAAGACTGT CAACTAGGCC CAGACATGGA AAAGTGGCTA 
TGCTTTCCCA AACAGGCAAT CTTTTCCATT ACGAACCCGC ACACGGATAT TGTGATGGTG 
GCACGAGTGG AGAAAGTTCT GATGGGAAAT ATTTCCAGTG GTGCAGAGCC CTACATCAAA 
AACACCGATT CCAGCAAGAC TGTACACAAG ATGCTGAAAT CCAATAAGCA GTTCTGTAGC 
AAACTAGGCA AGTACCGAAT GCCTTTCGCC TGGTCTGTAA GGTCAGTGTT TAAGGACAAC 
CAGGGCACAG TGGACAGGGA GTCTCGCTTC TCTCCTCTTT TCAAACAGGA AAGCAACAAG 
ATTTCCACCG AAGACCTCAT CAAGCTCATC ACCGAGTACA GGAGGGCGGA GAAGGCCAGT 
AAACTCCAGA CCATCCCTGG AAACCTTGAA ATAAATGTGG ACTGTGTTCC CATGGAATGT 
CCCAATTGTG TGACGTCCTC ATATGTTGCC CTGAAGCCGT TTGAGGACTG CAGCCAACAT 
GCTCCTACGG TGGAAATCGA TGAGTTCCTG CAGGATTCGT CAAAGTTCGC TCAACCTTAC 
AGAGTCTACA AGAACCACAT CTACATCTAC CCTAAACACC TCAAGTATGA CAGCCAGAAG 
AGCTTTGCTA AGGCTCGAAA TATTGCAGTT TGTGTGGAGT TTCGAAGCTC CGATGAAGAA 
CATGCCAAGC CGCTTAAGTG CATCTATGGA AAGCCCGGAG GCCCAGTTTT TACCACGACT 
GCCTGTACCA CAGTTCTGCA CCATTCACAG AACCCAGACT TTTATGATGA GGTCAAGATT 
GAGTTGCCCA CACATCTTCA TGAGAAACAC CATATACTCT TCTCCTTCTA TCATGTGACC 
TGTGACATCA ATGCCAAGAC CAGCTCTAAG AAGAAAGAGG CTCTTGAATT GCCAGTGGGT 
TATGCATGGC TGCCTCTTCT AAAGGATGGA CGCATTTCCT CTCAGGACTT CAGCATTCCT 
GTCTCCTGCA CTTTGCCTGC TGGCTATTTA CACATTAAAG AGCCTTCGTC CACCAAGAAT 
GGCTCTGATG TGAAGTGGGT CGATGGAGGG AAGCAGATCT TCAAATTGTC CACTTTGGTG 
CTTTCTACAG TGTATACTCA GGATCCCCAT CTAAACCGCT TCTTCCAGCA GTGCCAAAAA 
CGAGAGGCAG AAATCTCTCA ACCTCCTACC TCAAACTTCC TCAACTGCCT TAAAGGGCTC 
TTGAGCATGG AGAAGATTCA TGCCATAATT CGCTTCCTTC CTGTTATCTT CAACCAGCTC 
TTTAAAGTGC TTACGCAAAA CGACTCTGAT GAAGTCACCA CCAGCACCAC CAGGGTCCTG 
ATACACATCT TAGCCAAGTG CCACGAGGAG AACCTGGACC ACTATTTGCA CTCCTACATT 
AAGTTTGTCT TTAAGACTGA AGCGTGTGGC TTCAGAACCA TTCATGAAGA GCTGGCCAAG 
GGAATGACCT CTGACCTGAA GAGCAATGAA CAGTTAGTCA TACGCAACGT CCTGAAGTTC 
TCGTGGTTCT TTCTCGAGAT CATAGTGAAG TCAATGGCTC AGCATCTGCT GGACTCAAAC 
AAGCTCAAGC TGCCGCGACC GCAACGATTT CCTGGCACTT TCCAAAACCG TTTGGAGACG 
TTGATCATGA CCATGTCGGA TCACATTTAC TGGAAGAACA AAGAGCTGGC GGAGGAGACG 
CGGAGTGCAA ATATGGCCAT TGCGTTTTTT GTGAAGCGGT GCTTCACCTT GATGGACAGA 
GGTTTCACCT TTAAACTGAT CAGCAACTAC CTGAACATGA TCACAGCCAC TGACAATAAG 
GCGCTGTGTG AGCTGAAGTT TGAGTTCATA AGGGAAGTGT GTAACTATGA GCACTTTGTC 
CCTTTGAGTC TTCCCATCCC GTCTGCACGA ATCACAGACA ATGCATCAAC AGAAGCCCAA 
AGTACTAAGG TGGACTTCCC AGAGTACAGT TTGACAGAGG ATTTCTGCAG GAAGCATTTT 
CTGTCTGGAT TGTTGTTGCG AGAGCTGGGA CTCGCTTTGC AGGATGAGCA AGATCTGAGA 
CACATCGCTC TGGCCTGCCT GAAGAACCTG ATGGCCAAAC ACTCGCTGGA CGCTCGATAC 
GCCACAAAGG AGAAACAGGC TCGGATAGCT TCTCTTTACC TGCCGCTTTA TGGCCTGATC 
CTGGACAACA TGCCTCGGTT TTTCCTCAGA GATCTGTTTC CAATCTGCCT GACAGCGAGT 
GACCAGGGAT CCAGAGATGA TCTCAGTGTT GGAGTAGGTT TGGGTGGTCA GGTGACCCAT 
GTTTTACGCC ATGGAAACTC AATGGACGCG TCCTTCTCCA AGGAAGTGCT CAACTCCATT 
ACAGCCTTTT CATCATTGGC TGTATCGACC GCTAATCATG CCGACTCCAG GGGTTCTCTG 
ATCAGCATCG AGTCGAACCC CAGCAACAGT GACCGCAACA GTGAGAAGAT GGATGCCTGT 
GAAAAGTTTG CCAGGCCTCA GTCTCTTATT GGCTTTGGAT CCCGCTTTGA TAAACTGGAT 
CAAGCTGAAA CCAGAAGTCT GCTCATGTGT TTCCTGCACA TTATGAAGAC CATCTCAGAG 
GAGGTGATGG TTTCATACTG GCATCGAGCG ATTCACCAGG AGATCAGTGA CTTCTTCAAT 
ATTTTGGAAT TATGCCTTCA GCACTTTCGT TTCCTTGGAA AGCGGCACAT TGCCAGGAAG 
CTAGCGGCCG CAGTTAAGCT GGCTCAGGCC ACACAGAACA ATGGCACCCT GAAGGGCTCT 
AATGTGTCCT ACCACTCTCC AGGGCTCCTA CCCCAATGGA TTCTGTCTGC ACAGGACGGT 
CACAGACACG CTCGCTCTCA GACCATGCCC ATCATCAGGG GGAAAAACGC CCTCACAAAC 
CCCAAACTGC TGCATATGAT GGAGACAGAT GGAAATGTTG CTGAAGGAGA AAACTTTAAC 
CCTACAGACG TGGAGGCGAA CCTTTCTACT GAAGTAGCAC TGACCGTGTT GGATGTTCTG 
GACCTGTTTG TTCGACATCA TAAGAAACAG TTGCAGCACG ATGAAGGACA AAACTCTTTG 
ATGAAGAAAG TGTTCGACAC GTACCTGCTG TTCTTCCAGA TCAACCAGTC CACCACCACC 
CTCAGACACG TGTTCACAGC CCTGCGCCTC TTCATGCAGA AGTTTCCGTC CACGTTTTTC 
CAGGGTAAAG CGGATGTGTG TGGCAGCCTG TGTTACGAGA TCCTGAAGTG CTGTAACCAT 
CGCTCTAGCT CCACACAGAC TGAAGCCGCC GCTCTGCTTT ATTTCCTCAT GAGGAGGAAC 
TTTGAGTTCA CGAAGGGCAA GTGCATCGTC CGCTCACACC TGCAGGTGAT TAAAGCTGTG 
AGTCAGCTGA TCGCGGATGC AGGCATTGGC GGATCACGCT TCCAGCAGTC TCTGGCCATC 
ATCAATAACT TCGCCAATGG GGACACGCCA CTTAAAAACA CCACATTCCC AGCTGACGTA 
AAGGACCTGA CCAAGAGGAT ACGCACCGTC CTGATGGCCA CGTCTCAAAT GAAAGAGCAT 
GAAAAAGACC CGGAAATGCT GGTGGACCTT CAGTACAGTC TGGCCAACTC CTACGCCAGC 
ACACCTGAGC TCAGACGCAC CTGGCTGGAA AGCATGGCCA AAATACACGT CAGAAATGGA 
GATCTGTCTG AGGCAGCCAT GTGCTACATC CACATCTCAG CCCTCATAGC AGAGTCTCTG 
AGGAGAAGAG GTTACTGGAA GTCTGAAAAG GGTCGGATGT CAAGTGTGAG CAAAGAGGGA 
GCCTGTGTAA ATAACTGTAG CCCTTTACTA ACTCCCCAAG ACGGAGAAAC ATCATTCAGT 
ATGGGTTGGG CGGCATTTAC GAATATCTCG CCTAACGTTA AAGAAGAGGG AGCAATGAAA 
GAAGACGCTG GCACTCAGGA TACTCCATAC ACTGAGGACA CGCTGGTGGA GCAGCTGGAG 
ATGTGTGTTG ATTATCTCTG GAAGTCTGAG AGATATGAAC TTATTGCCGA GATCAACAAA 
CCTGTTATTG TCGTCTTTGA GAAGAGAAGA GATTTCAAGC GTCTATCAGA GCTGTACTAT 
GACATTCACC GCTCATACCT GAAGGTGACC GAAGTCGTGA ATTCTGAGAA ACGTCTGTTT 
GGTCGGTACT ATCGCGTGGC TTTCTACGGA CAGGGGTTTT TTGAGGAAGA GGAGAGTAAG 
GAGTTTATCT ACAAGGAGCC CAAACTCACA GGACTGTCCG AAATTTCTCA AAGACTCCTC 
AAACTCTACT CGGACAAGTT TGGAGCTGAT AACGTCAAAA TGATCCAGGA CTCCAACAAG 
GTGAATCCGA AAGACTTGGA CCCCAGATTT GCCTACATCC AGGTCACCTA TGTAGTGCCT 
TATTTTAATG AGAAGGAACA GCTGGAGAAG AAAACCGACT TCGAGCGCCA TCACAACATC 
GACCGATTTG TGTTTGAGAC GCCCTTCACG CTTTCTGGAA AAAAACACGG AGACGTGGAG 
GAACAGTGCA AAAGGAAAAC CATCCTGACC ACGAGCTCTA GTTTCCCTTT CTTGAAGAAG 
CGTATAGAGG TGGTGGAGCA ACAGAGTACC GAGATGAACC CTATTGAGGT GGCGATAGAC 
GAGATGAGCC GAAAAGTGTC TGAACTCAAA CAGCTGTGCA ATATGGAGGA GGTGGACATG 
ATCCGTCTGC AGCTCAAACT ACAGGGCAGC GTCAGCGTCA AGGTAAATGC AGGACCCATG 
GCCTACGCCC GAGCCTTCCT AGAGGAGAAA AATGCTAAGA AATACCCAGA CAACCAGGTC 
AAACTGCTGA AAGAGATCTT CAGACAGTTT GCAGAAGCGT GTGGACAGGC TCTGAGTGTG 
AACGAGCGGC TAATAAAAGA GGATCAGTTT GAGTATCAGA GCGAGATGAA AGCTCACTAT 
AAAGACATGC TCACGGAGCT CTCCGCCATT ATGAATGAGC AGATACCGTA CACGAGACAA 
CCAGGAACAG AGCGACACAG TGCCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOCK10 ( XM_009291414.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009289689.1 Danio rerio dedicator of cytokinesis 10 (dock10), transcript variant X4, mRNA.

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CloneID	ODa47523
Clone ID Related Accession (Same CDS sequence)	XM_009291414.2
Accession Version	XM_009291414.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	dedicator of cytokinesis protein 10 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291414.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAAAA TCACCTTTCG GAAGAAGATA AAGAGCAGAC GGGTTCGGGC AGACTGCAAC 
CGAGTGAGCT GTCAGGAGAA GCCCAAACTG ATCGACCCTC TGGACTATGA AGCTGTGATT 
TCTGAACTTG AAGCAGAATT CAGAGAAGAT CCTCTTCAAG ATCTCCTGTT ATTCCCAGAC 
CTTGACTTCA CTGTGTCCAC ACTTCCTGTA GAAAGGAGAA CAGTGAAGTC TACTGTCCCA 
GAGGGAGCTG ATGAACATGC TGAATGTCTG CTGGTCAGAC AGGCCTGCCA ATACTTCAAC 
TCCCAGCTGC ACATTGTTCA GTATAAATAT GATGAGTATG GAGGGGACTA CCGCATGCTG 
CCCAGGAAGC TGTGTAAAGC AGAAAAGTTG CCTTCCCACT CTTTTGAGAT TGACAATGAA 
GATGCGGACA AAGATGAGGA CACAACATCT TTGTCCTCAT CTAAAGGAGG GGGCGGCGGT 
GCAGGCAGTG GAGGCGGAGG AGGAATTGGT GTTTTTAAGT CCGGCTGGCT CTATAAAGGC 
AACTTCAACA GCACCGTCAA CAACAGCATC ACAGTTCGGT CCTTCAAGCG CCGATATTTT 
CAGCTGACTC AGCTCTCTGA CAACTCTTAC ATCATGAACT TCTATAAAGA TGAGAAAATT 
TCCAAGGAAC CGAAAGGCTG TATTTTTCTG GACTCCTGCA CCGGGGTGGT TCAGAATAAT 
CGACTGAGGA AACATGCGTT TGAGCTGAAA ATGAACGAAG TGACATATTT TGTCCTGGCT 
GCCGAGAATG AGCAAGACAT GGACGACTGG ATCAACACTT TAAATCGCAT CCTGCAGATC 
AGCCCTCCAG ACGGCCCGGC TCAGGACCGC AAGAGTACGG ACATGAGCGA CTCTAAAAAC 
GAGATCAGTG ATATACCACA AAATAACGTC CCTGTTCATA AAAACGAAGA AACAACTGAA 
AATGAGATCC ACCCTGAACT AGCCAAGTAT ATTTCTGAGA CAGAAGAAAG CTCGAGAGCT 
GTCAGAGCAG AGGAGAGACT GAACCTGTTC TCCCTGGACC CTGATATTCC TGTTTTAAGG 
AGTCCTAGGA CAGAAACCGG GGAGTCTTCT TCTGTCCATC CATTTGATGA GAAGCTTGGG 
AAGAGGCTGA TGATCTGCTG TCGCTCACTG AACCTCACAC TGCAAGGCTG CGTTAATGAA 
GCGGACACCG AACCCACCAC TAATATCGAG CCTTTCTTTG TGTCAGTGTG TCTGCTGGAC 
GTACGTGAGG GCAGGAAGGT TTCCGCTGAC TTCCATGTGG ACGTAAACCA TGAGTTAGTG 
CGGCAGATGC TTAATAGCTC TGGTAATCCG GGAGATCAAG GCTCGGATGT TGGTAATGGT 
GCACCGCTGG AAAATGGACT CACTTCCCCG GTAGAAAAGA AACCAGAAGA CTGTCAACTA 
GGCCCAGACA TGGAAAAGTG GCTATGCTTT CCCAAACAGG CAATCTTTTC CATTACGAAC 
CCGCACACGG ATATTGTGAT GGTGGCACGA GTGGAGAAAG TTCTGATGGG AAATATTTCC 
AGTGGTGCAG AGCCCTACAT CAAAAACACC GATTCCAGCA AGACTGTACA CAAGATGCTG 
AAATCCAATA AGCAGTTCTG TAGCAAACTA GGCAAGTACC GAATGCCTTT CGCCTGGTCT 
GTAAGGTCAG TGTTTAAGGA CAACCAGGGC ACAGTGGACA GGGAGTCTCG CTTCTCTCCT 
CTTTTCAAAC AGGAAAGCAA CAAGATTTCC ACCGAAGACC TCATCAAGCT CATCACCGAG 
TACAGGAGGG CGGAGAAGGC CAGTAAACTC CAGACCATCC CTGGAAACCT TGAAATAAAT 
GTGGACTGTG TTCCCATGGA ATGTCCCAAT TGTGTGACGT CCTCATATGT TGCCCTGAAG 
CCGTTTGAGG ACTGCAGCCA ACATGCTCCT ACGGTGGAAA TCGATGAGTT CCTGCAGGAT 
TCGTCAAAGT TCGCTCAACC TTACAGAGTC TACAAGAACC ACATCTACAT CTACCCTAAA 
CACCTCAAGT ATGACAGCCA GAAGAGCTTT GCTAAGGCTC GAAATATTGC AGTTTGTGTG 
GAGTTTCGAA GCTCCGATGA AGAACATGCC AAGCCGCTTA AGTGCATCTA TGGAAAGCCC 
GGAGGCCCAG TTTTTACCAC GACTGCCTGT ACCACAGTTC TGCACCATTC ACAGAACCCA 
GACTTTTATG ATGAGGTCAA GATTGAGTTG CCCACACATC TTCATGAGAA ACACCATATA 
CTCTTCTCCT TCTATCATGT GACCTGTGAC ATCAATGCCA AGACCAGCTC TAAGAAGAAA 
GAGGCTCTTG AATTGCCAGT GGGTTATGCA TGGCTGCCTC TTCTAAAGGA TGGACGCATT 
TCCTCTCAGG ACTTCAGCAT TCCTGTCTCC TGCACTTTGC CTGCTGGCTA TTTACACATT 
AAAGAGCCTT CGTCCACCAA GAATGGCTCT GATGTGAAGT GGGTCGATGG AGGGAAGCAG 
ATCTTCAAAT TGTCCACTTT GGTGCTTTCT ACAGTGTATA CTCAGGATCC CCATCTAAAC 
CGCTTCTTCC AGCAGTGCCA AAAACGAGAG GCAGAAATCT CTCAACCTCC TACCTCAAAC 
TTCCTCAACT GCCTTAAAGG GCTCTTGAGC ATGGAGAAGA TTCATGCCAT AATTCGCTTC 
CTTCCTGTTA TCTTCAACCA GCTCTTTAAA GTGCTTACGC AAAACGACTC TGATGAAGTC 
ACCACCAGCA CCACCAGGGT CCTGATACAC ATCTTAGCCA AGTGCCACGA GGAGAACCTG 
GACCACTATT TGCACTCCTA CATTAAGTTT GTCTTTAAGA CTGAAGCGTG TGGCTTCAGA 
ACCATTCATG AAGAGCTGGC CAAGGGAATG ACCTCTGACC TGAAGAGCAA TGAACAGTTA 
GTCATACGCA ACGTCCTGAA GTTCTCGTGG TTCTTTCTCG AGATCATAGT GAAGTCAATG 
GCTCAGCATC TGCTGGACTC AAACAAGCTC AAGCTGCCGC GACCGCAACG ATTTCCTGGC 
ACTTTCCAAA ACCGTTTGGA GACGTTGATC ATGACCATGT CGGATCACAT TTACTGGAAG 
AACAAAGAGC TGGCGGAGGA GACGCGGAGT GCAAATATGG CCATTGCGTT TTTTGTGAAG 
CGGTGCTTCA CCTTGATGGA CAGAGGTTTC ACCTTTAAAC TGATCAGCAA CTACCTGAAC 
ATGATCACAG CCACTGACAA TAAGGCGCTG TGTGAGCTGA AGTTTGAGTT CATAAGGGAA 
GTGTGTAACT ATGAGCACTT TGTCCCTTTG AGTCTTCCCA TCCCGTCTGC ACGAATCACA 
GACAATGCAT CAACAGAAGC CCAAAGTACT AAGGTGGACT TCCCAGAGTA CAGTTTGACA 
GAGGATTTCT GCAGGAAGCA TTTTCTGTCT GGATTGTTGT TGCGAGAGCT GGGACTCGCT 
TTGCAGGATG AGCAAGATCT GAGACACATC GCTCTGGCCT GCCTGAAGAA CCTGATGGCC 
AAACACTCGC TGGACGCTCG ATACGCCACA AAGGAGAAAC AGGCTCGGAT AGCTTCTCTT 
TACCTGCCGC TTTATGGCCT GATCCTGGAC AACATGCCTC GGTTTTTCCT CAGAGATCTG 
TTTCCAATCT GCCTGACAGC GAGTGACCAG GGATCCAGAG ATGATCTCAG TGTTGGAGTA 
GGTTTGGGTG GTCAGGTGAC CCATGTTTTA CGCCATGGAA ACTCAATGGA CGCGTCCTTC 
TCCAAGGAAG TGCTCAACTC CATTACAGCC TTTTCATCAT TGGCTGTATC GACCGCTAAT 
CATGCCGACT CCAGGGGTTC TCTGATCAGC ATCGAGTCGA ACCCCAGCAA CAGTGACCGC 
AACAGTGAGA AGATGGATGC CTGTGAAAAG TTTGCCAGGC CTCAGTCTCT TATTGGCTTT 
GGATCCCGCT TTGATAAACT GGATCAAGCT GAAACCAGAA GTCTGCTCAT GTGTTTCCTG 
CACATTATGA AGACCATCTC AGAGGAGGTG ATGGTTTCAT ACTGGCATCG AGCGATTCAC 
CAGGAGATCA GTGACTTCTT CAATATTTTG GAATTATGCC TTCAGCACTT TCGTTTCCTT 
GGAAAGCGGC ACATTGCCAG GAAGCTAGCG GCCGCAGTTA AGCTGGCTCA GGCCACACAG 
AACAATGGCA CCCTGAAGGG CTCTAATGTG TCCTACCACT CTCCAGGGCT CCTACCCCAA 
TGGATTCTGT CTGCACAGGA CGGTCACAGA CACGCTCGCT CTCAGACCAT GCCCATCATC 
AGGGGGAAAA ACGCCCTCAC AAACCCCAAA CTGCTGCATA TGATGGAGAC AGATGGAAAT 
GTTGCTGAAG GAGAAAACTT TAACCCTACA GACGTGGAGG CGAACCTTTC TACTGAAGTA 
GCACTGACCG TGTTGGATGT TCTGGACCTG TTTGTTCGAC ATCATAAGAA ACAGTTGCAG 
CACGATGAAG GACAAAACTC TTTGATGAAG AAAGTGTTCG ACACGTACCT GCTGTTCTTC 
CAGATCAACC AGTCCACCAC CACCCTCAGA CACGTGTTCA CAGCCCTGCG CCTCTTCATG 
CAGAAGTTTC CGTCCACGTT TTTCCAGGGT AAAGCGGATG TGTGTGGCAG CCTGTGTTAC 
GAGATCCTGA AGTGCTGTAA CCATCGCTCT AGCTCCACAC AGACTGAAGC CGCCGCTCTG 
CTTTATTTCC TCATGAGGAG GAACTTTGAG TTCACGAAGG GCAAGTGCAT CGTCCGCTCA 
CACCTGCAGG TGATTAAAGC TGTGAGTCAG CTGATCGCGG ATGCAGGCAT TGGCGGATCA 
CGCTTCCAGC AGTCTCTGGC CATCATCAAT AACTTCGCCA ATGGGGACAC GCCACTTAAA 
AACACCACAT TCCCAGCTGA CGTAAAGGAC CTGACCAAGA GGATACGCAC CGTCCTGATG 
GCCACGTCTC AAATGAAAGA GCATGAAAAA GACCCGGAAA TGCTGGTGGA CCTTCAGTAC 
AGTCTGGCCA ACTCCTACGC CAGCACACCT GAGCTCAGAC GCACCTGGCT GGAAAGCATG 
GCCAAAATAC ACGTCAGAAA TGGAGATCTG TCTGAGGCAG CCATGTGCTA CATCCACATC 
TCAGCCCTCA TAGCAGAGTC TCTGAGGAGA AGAGGTTACT GGAAGTCTGA AAAGGGTCGG 
ATGTCAAGTG TGAGCAAAGA GGGAGCCTGT GTAAATAACT GTAGCCCTTT ACTAACTCCC 
CAAGACGGAG AAACATCATT CAGTATGGGT TGGGCGGCAT TTACGAATAT CTCGCCTAAC 
GTTAAAGAAG AGGGAGCAAT GAAAGAAGAC GCTGGCACTC AGGATACTCC ATACACTGAG 
GACACGCTGG TGGAGCAGCT GGAGATGTGT GTTGATTATC TCTGGAAGTC TGAGAGATAT 
GAACTTATTG CCGAGATCAA CAAACCTGTT ATTGTCGTCT TTGAGAAGAG AAGAGATTTC 
AAGCGTCTAT CAGAGCTGTA CTATGACATT CACCGCTCAT ACCTGAAGGT GACCGAAGTC 
GTGAATTCTG AGAAACGTCT GTTTGGTCGG TACTATCGCG TGGCTTTCTA CGGACAGGGG 
TTTTTTGAGG AAGAGGAGAG TAAGGAGTTT ATCTACAAGG AGCCCAAACT CACAGGACTG 
TCCGAAATTT CTCAAAGACT CCTCAAACTC TACTCGGACA AGTTTGGAGC TGATAACGTC 
AAAATGATCC AGGACTCCAA CAAGGTGAAT CCGAAAGACT TGGACCCCAG ATTTGCCTAC 
ATCCAGGTCA CCTATGTAGT GCCTTATTTT AATGAGAAGG AACAGCTGGA GAAGAAAACC 
GACTTCGAGC GCCATCACAA CATCGACCGA TTTGTGTTTG AGACGCCCTT CACGCTTTCT 
GGAAAAAAAC ACGGAGACGT GGAGGAACAG TGCAAAAGGA AAACCATCCT GACCACGAGC 
TCTAGTTTCC CTTTCTTGAA GAAGCGTATA GAGGTGGTGG AGCAACAGAG TACCGAGATG 
AACCCTATTG AGGTGGCGAT AGACGAGATG AGCCGAAAAG TGTCTGAACT CAAACAGCTG 
TGCAATATGG AGGAGGTGGA CATGATCCGT CTGCAGCTCA AACTACAGGG CAGCGTCAGC 
GTCAAGGTAA ATGCAGGACC CATGGCCTAC GCCCGAGCCT TCCTAGAGGA GAAAAATGCT 
AAGAAATACC CAGACAACCA GGTCAAACTG CTGAAAGAGA TCTTCAGACA GTTTGCAGAA 
GCGTGTGGAC AGGCTCTGAG TGTGAACGAG CGGCTAATAA AAGAGGATCA GTTTGAGTAT 
CAGAGCGAGA TGAAAGCTCA CTATAAAGAC ATGCTCACGG AGCTCTCCGC CATTATGAAT 
GAGCAGATAC CGTACACGAG ACAACCAGGA ACAGAGCGAC ACAGTGCCAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289689.1
CDS	115..6588
Translation	MSKITFRKKIKSRRVRADCNRVSCQEKPKLIDPLDYEAVISELEAEFREDPLQDLLLFPDLDFTVSTLPVERRTVKSTVPEGADEHAECLLVRQACQYFNSQLHIVQYKYDEYGGDYRMLPRKLCKAEKLPSHSFEIDNEDADKDEDTTSLSSSKGGGGGAGSGGGGGIGVFKSGWLYKGNFNSTVNNSITVRSFKRRYFQLTQLSDNSYIMNFYKDEKISKEPKGCIFLDSCTGVVQNNRLRKHAFELKMNEVTYFVLAAENEQDMDDWINTLNRILQISPPDGPAQDRKSTDMSDSKNEISDIPQNNVPVHKNEETTENEIHPELAKYISETEESSRAVRAEERLNLFSLDPDIPVLRSPRTETGESSSVHPFDEKLGKRLMICCRSLNLTLQGCVNEADTEPTTNIEPFFVSVCLLDVREGRKVSADFHVDVNHELVRQMLNSSGNPGDQGSDVGNGAPLENGLTSPVEKKPEDCQLGPDMEKWLCFPKQAIFSITNPHTDIVMVARVEKVLMGNISSGAEPYIKNTDSSKTVHKMLKSNKQFCSKLGKYRMPFAWSVRSVFKDNQGTVDRESRFSPLFKQESNKISTEDLIKLITEYRRAEKASKLQTIPGNLEINVDCVPMECPNCVTSSYVALKPFEDCSQHAPTVEIDEFLQDSSKFAQPYRVYKNHIYIYPKHLKYDSQKSFAKARNIAVCVEFRSSDEEHAKPLKCIYGKPGGPVFTTTACTTVLHHSQNPDFYDEVKIELPTHLHEKHHILFSFYHVTCDINAKTSSKKKEALELPVGYAWLPLLKDGRISSQDFSIPVSCTLPAGYLHIKEPSSTKNGSDVKWVDGGKQIFKLSTLVLSTVYTQDPHLNRFFQQCQKREAEISQPPTSNFLNCLKGLLSMEKIHAIIRFLPVIFNQLFKVLTQNDSDEVTTSTTRVLIHILAKCHEENLDHYLHSYIKFVFKTEACGFRTIHEELAKGMTSDLKSNEQLVIRNVLKFSWFFLEIIVKSMAQHLLDSNKLKLPRPQRFPGTFQNRLETLIMTMSDHIYWKNKELAEETRSANMAIAFFVKRCFTLMDRGFTFKLISNYLNMITATDNKALCELKFEFIREVCNYEHFVPLSLPIPSARITDNASTEAQSTKVDFPEYSLTEDFCRKHFLSGLLLRELGLALQDEQDLRHIALACLKNLMAKHSLDARYATKEKQARIASLYLPLYGLILDNMPRFFLRDLFPICLTASDQGSRDDLSVGVGLGGQVTHVLRHGNSMDASFSKEVLNSITAFSSLAVSTANHADSRGSLISIESNPSNSDRNSEKMDACEKFARPQSLIGFGSRFDKLDQAETRSLLMCFLHIMKTISEEVMVSYWHRAIHQEISDFFNILELCLQHFRFLGKRHIARKLAAAVKLAQATQNNGTLKGSNVSYHSPGLLPQWILSAQDGHRHARSQTMPIIRGKNALTNPKLLHMMETDGNVAEGENFNPTDVEANLSTEVALTVLDVLDLFVRHHKKQLQHDEGQNSLMKKVFDTYLLFFQINQSTTTLRHVFTALRLFMQKFPSTFFQGKADVCGSLCYEILKCCNHRSSSTQTEAAALLYFLMRRNFEFTKGKCIVRSHLQVIKAVSQLIADAGIGGSRFQQSLAIINNFANGDTPLKNTTFPADVKDLTKRIRTVLMATSQMKEHEKDPEMLVDLQYSLANSYASTPELRRTWLESMAKIHVRNGDLSEAAMCYIHISALIAESLRRRGYWKSEKGRMSSVSKEGACVNNCSPLLTPQDGETSFSMGWAAFTNISPNVKEEGAMKEDAGTQDTPYTEDTLVEQLEMCVDYLWKSERYELIAEINKPVIVVFEKRRDFKRLSELYYDIHRSYLKVTEVVNSEKRLFGRYYRVAFYGQGFFEEEESKEFIYKEPKLTGLSEISQRLLKLYSDKFGADNVKMIQDSNKVNPKDLDPRFAYIQVTYVVPYFNEKEQLEKKTDFERHHNIDRFVFETPFTLSGKKHGDVEEQCKRKTILTTSSSFPFLKKRIEVVEQQSTEMNPIEVAIDEMSRKVSELKQLCNMEEVDMIRLQLKLQGSVSVKVNAGPMAYARAFLEEKNAKKYPDNQVKLLKEIFRQFAEACGQALSVNERLIKEDQFEYQSEMKAHYKDMLTELSAIMNEQIPYTRQPGTERHSAI

Target ORF information:

RefSeq Version	XM_009291414.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio dedicator of cytokinesis 10 (dock10), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291414.2

ORF Insert Sequence:

ATGAGTAAAA TCACCTTTCG GAAGAAGATA AAGAGCAGAC GGGTTCGGGC AGACTGCAAC 
CGAGTGAGCT GTCAGGAGAA GCCCAAACTG ATCGACCCTC TGGACTATGA AGCTGTGATT 
TCTGAACTTG AAGCAGAATT CAGAGAAGAT CCTCTTCAAG ATCTCCTGTT ATTCCCAGAC 
CTTGACTTCA CTGTGTCCAC ACTTCCTGTA GAAAGGAGAA CAGTGAAGTC TACTGTCCCA 
GAGGGAGCTG ATGAACATGC TGAATGTCTG CTGGTCAGAC AGGCCTGCCA ATACTTCAAC 
TCCCAGCTGC ACATTGTTCA GTATAAATAT GATGAGTATG GAGGGGACTA CCGCATGCTG 
CCCAGGAAGC TGTGTAAAGC AGAAAAGTTG CCTTCCCACT CTTTTGAGAT TGACAATGAA 
GATGCGGACA AAGATGAGGA CACAACATCT TTGTCCTCAT CTAAAGGAGG GGGCGGCGGT 
GCAGGCAGTG GAGGCGGAGG AGGAATTGGT GTTTTTAAGT CCGGCTGGCT CTATAAAGGC 
AACTTCAACA GCACCGTCAA CAACAGCATC ACAGTTCGGT CCTTCAAGCG CCGATATTTT 
CAGCTGACTC AGCTCTCTGA CAACTCTTAC ATCATGAACT TCTATAAAGA TGAGAAAATT 
TCCAAGGAAC CGAAAGGCTG TATTTTTCTG GACTCCTGCA CCGGGGTGGT TCAGAATAAT 
CGACTGAGGA AACATGCGTT TGAGCTGAAA ATGAACGAAG TGACATATTT TGTCCTGGCT 
GCCGAGAATG AGCAAGACAT GGACGACTGG ATCAACACTT TAAATCGCAT CCTGCAGATC 
AGCCCTCCAG ACGGCCCGGC TCAGGACCGC AAGAGTACGG ACATGAGCGA CTCTAAAAAC 
GAGATCAGTG ATATACCACA AAATAACGTC CCTGTTCATA AAAACGAAGA AACAACTGAA 
AATGAGATCC ACCCTGAACT AGCCAAGTAT ATTTCTGAGA CAGAAGAAAG CTCGAGAGCT 
GTCAGAGCAG AGGAGAGACT GAACCTGTTC TCCCTGGACC CTGATATTCC TGTTTTAAGG 
AGTCCTAGGA CAGAAACCGG GGAGTCTTCT TCTGTCCATC CATTTGATGA GAAGCTTGGG 
AAGAGGCTGA TGATCTGCTG TCGCTCACTG AACCTCACAC TGCAAGGCTG CGTTAATGAA 
GCGGACACCG AACCCACCAC TAATATCGAG CCTTTCTTTG TGTCAGTGTG TCTGCTGGAC 
GTACGTGAGG GCAGGAAGGT TTCCGCTGAC TTCCATGTGG ACGTAAACCA TGAGTTAGTG 
CGGCAGATGC TTAATAGCTC TGGTAATCCG GGAGATCAAG GCTCGGATGT TGGTAATGGT 
GCACCGCTGG AAAATGGACT CACTTCCCCG GTAGAAAAGA AACCAGAAGA CTGTCAACTA 
GGCCCAGACA TGGAAAAGTG GCTATGCTTT CCCAAACAGG CAATCTTTTC CATTACGAAC 
CCGCACACGG ATATTGTGAT GGTGGCACGA GTGGAGAAAG TTCTGATGGG AAATATTTCC 
AGTGGTGCAG AGCCCTACAT CAAAAACACC GATTCCAGCA AGACTGTACA CAAGATGCTG 
AAATCCAATA AGCAGTTCTG TAGCAAACTA GGCAAGTACC GAATGCCTTT CGCCTGGTCT 
GTAAGGTCAG TGTTTAAGGA CAACCAGGGC ACAGTGGACA GGGAGTCTCG CTTCTCTCCT 
CTTTTCAAAC AGGAAAGCAA CAAGATTTCC ACCGAAGACC TCATCAAGCT CATCACCGAG 
TACAGGAGGG CGGAGAAGGC CAGTAAACTC CAGACCATCC CTGGAAACCT TGAAATAAAT 
GTGGACTGTG TTCCCATGGA ATGTCCCAAT TGTGTGACGT CCTCATATGT TGCCCTGAAG 
CCGTTTGAGG ACTGCAGCCA ACATGCTCCT ACGGTGGAAA TCGATGAGTT CCTGCAGGAT 
TCGTCAAAGT TCGCTCAACC TTACAGAGTC TACAAGAACC ACATCTACAT CTACCCTAAA 
CACCTCAAGT ATGACAGCCA GAAGAGCTTT GCTAAGGCTC GAAATATTGC AGTTTGTGTG 
GAGTTTCGAA GCTCCGATGA AGAACATGCC AAGCCGCTTA AGTGCATCTA TGGAAAGCCC 
GGAGGCCCAG TTTTTACCAC GACTGCCTGT ACCACAGTTC TGCACCATTC ACAGAACCCA 
GACTTTTATG ATGAGGTCAA GATTGAGTTG CCCACACATC TTCATGAGAA ACACCATATA 
CTCTTCTCCT TCTATCATGT GACCTGTGAC ATCAATGCCA AGACCAGCTC TAAGAAGAAA 
GAGGCTCTTG AATTGCCAGT GGGTTATGCA TGGCTGCCTC TTCTAAAGGA TGGACGCATT 
TCCTCTCAGG ACTTCAGCAT TCCTGTCTCC TGCACTTTGC CTGCTGGCTA TTTACACATT 
AAAGAGCCTT CGTCCACCAA GAATGGCTCT GATGTGAAGT GGGTCGATGG AGGGAAGCAG 
ATCTTCAAAT TGTCCACTTT GGTGCTTTCT ACAGTGTATA CTCAGGATCC CCATCTAAAC 
CGCTTCTTCC AGCAGTGCCA AAAACGAGAG GCAGAAATCT CTCAACCTCC TACCTCAAAC 
TTCCTCAACT GCCTTAAAGG GCTCTTGAGC ATGGAGAAGA TTCATGCCAT AATTCGCTTC 
CTTCCTGTTA TCTTCAACCA GCTCTTTAAA GTGCTTACGC AAAACGACTC TGATGAAGTC 
ACCACCAGCA CCACCAGGGT CCTGATACAC ATCTTAGCCA AGTGCCACGA GGAGAACCTG 
GACCACTATT TGCACTCCTA CATTAAGTTT GTCTTTAAGA CTGAAGCGTG TGGCTTCAGA 
ACCATTCATG AAGAGCTGGC CAAGGGAATG ACCTCTGACC TGAAGAGCAA TGAACAGTTA 
GTCATACGCA ACGTCCTGAA GTTCTCGTGG TTCTTTCTCG AGATCATAGT GAAGTCAATG 
GCTCAGCATC TGCTGGACTC AAACAAGCTC AAGCTGCCGC GACCGCAACG ATTTCCTGGC 
ACTTTCCAAA ACCGTTTGGA GACGTTGATC ATGACCATGT CGGATCACAT TTACTGGAAG 
AACAAAGAGC TGGCGGAGGA GACGCGGAGT GCAAATATGG CCATTGCGTT TTTTGTGAAG 
CGGTGCTTCA CCTTGATGGA CAGAGGTTTC ACCTTTAAAC TGATCAGCAA CTACCTGAAC 
ATGATCACAG CCACTGACAA TAAGGCGCTG TGTGAGCTGA AGTTTGAGTT CATAAGGGAA 
GTGTGTAACT ATGAGCACTT TGTCCCTTTG AGTCTTCCCA TCCCGTCTGC ACGAATCACA 
GACAATGCAT CAACAGAAGC CCAAAGTACT AAGGTGGACT TCCCAGAGTA CAGTTTGACA 
GAGGATTTCT GCAGGAAGCA TTTTCTGTCT GGATTGTTGT TGCGAGAGCT GGGACTCGCT 
TTGCAGGATG AGCAAGATCT GAGACACATC GCTCTGGCCT GCCTGAAGAA CCTGATGGCC 
AAACACTCGC TGGACGCTCG ATACGCCACA AAGGAGAAAC AGGCTCGGAT AGCTTCTCTT 
TACCTGCCGC TTTATGGCCT GATCCTGGAC AACATGCCTC GGTTTTTCCT CAGAGATCTG 
TTTCCAATCT GCCTGACAGC GAGTGACCAG GGATCCAGAG ATGATCTCAG TGTTGGAGTA 
GGTTTGGGTG GTCAGGTGAC CCATGTTTTA CGCCATGGAA ACTCAATGGA CGCGTCCTTC 
TCCAAGGAAG TGCTCAACTC CATTACAGCC TTTTCATCAT TGGCTGTATC GACCGCTAAT 
CATGCCGACT CCAGGGGTTC TCTGATCAGC ATCGAGTCGA ACCCCAGCAA CAGTGACCGC 
AACAGTGAGA AGATGGATGC CTGTGAAAAG TTTGCCAGGC CTCAGTCTCT TATTGGCTTT 
GGATCCCGCT TTGATAAACT GGATCAAGCT GAAACCAGAA GTCTGCTCAT GTGTTTCCTG 
CACATTATGA AGACCATCTC AGAGGAGGTG ATGGTTTCAT ACTGGCATCG AGCGATTCAC 
CAGGAGATCA GTGACTTCTT CAATATTTTG GAATTATGCC TTCAGCACTT TCGTTTCCTT 
GGAAAGCGGC ACATTGCCAG GAAGCTAGCG GCCGCAGTTA AGCTGGCTCA GGCCACACAG 
AACAATGGCA CCCTGAAGGG CTCTAATGTG TCCTACCACT CTCCAGGGCT CCTACCCCAA 
TGGATTCTGT CTGCACAGGA CGGTCACAGA CACGCTCGCT CTCAGACCAT GCCCATCATC 
AGGGGGAAAA ACGCCCTCAC AAACCCCAAA CTGCTGCATA TGATGGAGAC AGATGGAAAT 
GTTGCTGAAG GAGAAAACTT TAACCCTACA GACGTGGAGG CGAACCTTTC TACTGAAGTA 
GCACTGACCG TGTTGGATGT TCTGGACCTG TTTGTTCGAC ATCATAAGAA ACAGTTGCAG 
CACGATGAAG GACAAAACTC TTTGATGAAG AAAGTGTTCG ACACGTACCT GCTGTTCTTC 
CAGATCAACC AGTCCACCAC CACCCTCAGA CACGTGTTCA CAGCCCTGCG CCTCTTCATG 
CAGAAGTTTC CGTCCACGTT TTTCCAGGGT AAAGCGGATG TGTGTGGCAG CCTGTGTTAC 
GAGATCCTGA AGTGCTGTAA CCATCGCTCT AGCTCCACAC AGACTGAAGC CGCCGCTCTG 
CTTTATTTCC TCATGAGGAG GAACTTTGAG TTCACGAAGG GCAAGTGCAT CGTCCGCTCA 
CACCTGCAGG TGATTAAAGC TGTGAGTCAG CTGATCGCGG ATGCAGGCAT TGGCGGATCA 
CGCTTCCAGC AGTCTCTGGC CATCATCAAT AACTTCGCCA ATGGGGACAC GCCACTTAAA 
AACACCACAT TCCCAGCTGA CGTAAAGGAC CTGACCAAGA GGATACGCAC CGTCCTGATG 
GCCACGTCTC AAATGAAAGA GCATGAAAAA GACCCGGAAA TGCTGGTGGA CCTTCAGTAC 
AGTCTGGCCA ACTCCTACGC CAGCACACCT GAGCTCAGAC GCACCTGGCT GGAAAGCATG 
GCCAAAATAC ACGTCAGAAA TGGAGATCTG TCTGAGGCAG CCATGTGCTA CATCCACATC 
TCAGCCCTCA TAGCAGAGTC TCTGAGGAGA AGAGGTTACT GGAAGTCTGA AAAGGGTCGG 
ATGTCAAGTG TGAGCAAAGA GGGAGCCTGT GTAAATAACT GTAGCCCTTT ACTAACTCCC 
CAAGACGGAG AAACATCATT CAGTATGGGT TGGGCGGCAT TTACGAATAT CTCGCCTAAC 
GTTAAAGAAG AGGGAGCAAT GAAAGAAGAC GCTGGCACTC AGGATACTCC ATACACTGAG 
GACACGCTGG TGGAGCAGCT GGAGATGTGT GTTGATTATC TCTGGAAGTC TGAGAGATAT 
GAACTTATTG CCGAGATCAA CAAACCTGTT ATTGTCGTCT TTGAGAAGAG AAGAGATTTC 
AAGCGTCTAT CAGAGCTGTA CTATGACATT CACCGCTCAT ACCTGAAGGT GACCGAAGTC 
GTGAATTCTG AGAAACGTCT GTTTGGTCGG TACTATCGCG TGGCTTTCTA CGGACAGGGG 
TTTTTTGAGG AAGAGGAGAG TAAGGAGTTT ATCTACAAGG AGCCCAAACT CACAGGACTG 
TCCGAAATTT CTCAAAGACT CCTCAAACTC TACTCGGACA AGTTTGGAGC TGATAACGTC 
AAAATGATCC AGGACTCCAA CAAGGTGAAT CCGAAAGACT TGGACCCCAG ATTTGCCTAC 
ATCCAGGTCA CCTATGTAGT GCCTTATTTT AATGAGAAGG AACAGCTGGA GAAGAAAACC 
GACTTCGAGC GCCATCACAA CATCGACCGA TTTGTGTTTG AGACGCCCTT CACGCTTTCT 
GGAAAAAAAC ACGGAGACGT GGAGGAACAG TGCAAAAGGA AAACCATCCT GACCACGAGC 
TCTAGTTTCC CTTTCTTGAA GAAGCGTATA GAGGTGGTGG AGCAACAGAG TACCGAGATG 
AACCCTATTG AGGTGGCGAT AGACGAGATG AGCCGAAAAG TGTCTGAACT CAAACAGCTG 
TGCAATATGG AGGAGGTGGA CATGATCCGT CTGCAGCTCA AACTACAGGG CAGCGTCAGC 
GTCAAGGTAA ATGCAGGACC CATGGCCTAC GCCCGAGCCT TCCTAGAGGA GAAAAATGCT 
AAGAAATACC CAGACAACCA GGTCAAACTG CTGAAAGAGA TCTTCAGACA GTTTGCAGAA 
GCGTGTGGAC AGGCTCTGAG TGTGAACGAG CGGCTAATAA AAGAGGATCA GTTTGAGTAT 
CAGAGCGAGA TGAAAGCTCA CTATAAAGAC ATGCTCACGG AGCTCTCCGC CATTATGAAT 
GAGCAGATAC CGTACACGAG ACAACCAGGA ACAGAGCGAC ACAGTGCCAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOCK10 ( XM_009291416.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009289691.1 Danio rerio dedicator of cytokinesis 10 (dock10), transcript variant X5, mRNA.

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CloneID	ODa47525
Clone ID Related Accession (Same CDS sequence)	XM_009291416.2
Accession Version	XM_009291416.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	dedicator of cytokinesis protein 10 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291416.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTGTA CCACGAGTCT ACTACAGGCT GTGAATTTTA TAATGAGGAG AAACCATTCA 
CGCGTCTTTT CTGACCAGCA GAAACAACAT TTGCTGGAAA CTGCACCGCT GGAGGAGGGG 
ATGGTCATCA GTGAACCACA CAGCACTGTC GTCATGCAGG AGAAGCCCAA ACTGATCGAC 
CCTCTGGACT ATGAAGCTGT GATTTCTGAA CTTGAAGCAG AATTCAGAGA AGATCCTCTT 
CAAGATCTCC TGTTATTCCC AGACCTTGAC TTCACTGTGT CCACACTTCC TGTAGAAAGG 
AGAACAGTGA AGTCTACTGT CCCAGAGGGA GCTGATGAAC ATGCTGAATG TCTGCTGGTC 
AGACAGGCCT GCCAATACTT CAACTCCCAG CTGCACATTG TTCAGTATAA ATATGATGAG 
TATGGAGGGG ACTACCGCAT GCTGCCCAGG AAGCTGTGTA AAGCAGAAAA GTTGCCTTCC 
CACTCTTTTG AGATTGACAA TGAAGATGCG GACAAAGATG AGGACACAAC ATCTTTGTCC 
TCATCTAAAG GAGGGGGCGG CGGTGCAGGC AGTGGAGGCG GAGGAGGAAT TGGTGTTTTT 
AAGTCCGGCT GGCTCTATAA AGGCAACTTC AACAGCACCG TCAACAACAG CATCACAGTT 
CGGTCCTTCA AGCGCCGATA TTTTCAGCTG ACTCAGCTCT CTGACAACTC TTACATCATG 
AACTTCTATA AAGATGAGAA AATTTCCAAG GAACCGAAAG GCTGTATTTT TCTGGACTCC 
TGCACCGGGG TGGTTCAGAA TAATCGACTG AGGAAACATG CGTTTGAGCT GAAAATGAAC 
GAAGTGACAT ATTTTGTCCT GGCTGCCGAG AATGAGCAAG ACATGGACGA CTGGATCAAC 
ACTTTAAATC GCATCCTGCA GATCAGCCCT CCAGACGGCC CGGCTCAGGA CCGCAAGAGT 
ACGGACATGA GCGACTCTAA AAACGAGATC AGTGATATAC CACAAAATAA CGTCCCTGTT 
CATAAAAACG AAGAAACAAC TGAAAATGAG ATCCACCCTG AACTAGCCAA GTATATTTCT 
GAGACAGAAG AAAGCTCGAG AGCTGTCAGA GCAGAGGAGA GACTGAACCT GTTCTCCCTG 
GACCCTGATA TTCCTGTTTT AAGGAGTCCT AGGACAGAAA CCGGGGAGTC TTCTTCTGTC 
CATCCATTTG ATGAGAAGCT TGGGAAGAGG CTGATGATCT GCTGTCGCTC ACTGAACCTC 
ACACTGCAAG GCTGCGTTAA TGAAGCGGAC ACCGAACCCA CCACTAATAT CGAGCCTTTC 
TTTGTGTCAG TGTGTCTGCT GGACGTACGT GAGGGCAGGA AGGTTTCCGC TGACTTCCAT 
GTGGACGTAA ACCATGAGTT AGTGCGGCAG ATGCTTAATA GCTCTGGTAA TCCGGGAGAT 
CAAGGCTCGG ATGTTGGTAA TGGTGCACCG CTGGAAAATG GACTCACTTC CCCGGTAGAA 
AAGAAACCAG AAGACTGTCA ACTAGGCCCA GACATGGAAA AGTGGCTATG CTTTCCCAAA 
CAGGCAATCT TTTCCATTAC GAACCCGCAC ACGGATATTG TGATGGTGGC ACGAGTGGAG 
AAAGTTCTGA TGGGAAATAT TTCCAGTGGT GCAGAGCCCT ACATCAAAAA CACCGATTCC 
AGCAAGACTG TACACAAGAT GCTGAAATCC AATAAGCAGT TCTGTAGCAA ACTAGGCAAG 
TACCGAATGC CTTTCGCCTG GTCTGTAAGG TCAGTGTTTA AGGACAACCA GGGCACAGTG 
GACAGGGAGT CTCGCTTCTC TCCTCTTTTC AAACAGGAAA GCAACAAGAT TTCCACCGAA 
GACCTCATCA AGCTCATCAC CGAGTACAGG AGGGCGGAGA AGGCCAGTAA ACTCCAGACC 
ATCCCTGGAA ACCTTGAAAT AAATGTGGAC TGTGTTCCCA TGGAATGTCC CAATTGTGTG 
ACGTCCTCAT ATGTTGCCCT GAAGCCGTTT GAGGACTGCA GCCAACATGC TCCTACGGTG 
GAAATCGATG AGTTCCTGCA GGATTCGTCA AAGTTCGCTC AACCTTACAG AGTCTACAAG 
AACCACATCT ACATCTACCC TAAACACCTC AAGTATGACA GCCAGAAGAG CTTTGCTAAG 
GCTCGAAATA TTGCAGTTTG TGTGGAGTTT CGAAGCTCCG ATGAAGAACA TGCCAAGCCG 
CTTAAGTGCA TCTATGGAAA GCCCGGAGGC CCAGTTTTTA CCACGACTGC CTGTACCACA 
GTTCTGCACC ATTCACAGAA CCCAGACTTT TATGATGAGG TCAAGATTGA GTTGCCCACA 
CATCTTCATG AGAAACACCA TATACTCTTC TCCTTCTATC ATGTGACCTG TGACATCAAT 
GCCAAGACCA GCTCTAAGAA GAAAGAGGCT CTTGAATTGC CAGTGGGTTA TGCATGGCTG 
CCTCTTCTAA AGGATGGACG CATTTCCTCT CAGGACTTCA GCATTCCTGT CTCCTGCACT 
TTGCCTGCTG GCTATTTACA CATTAAAGAG CCTTCGTCCA CCAAGAATGG CTCTGATGTG 
AAGTGGGTCG ATGGAGGGAA GCAGATCTTC AAATTGTCCA CTTTGGTGCT TTCTACAGTG 
TATACTCAGG ATCCCCATCT AAACCGCTTC TTCCAGCAGT GCCAAAAACG AGAGGCAGAA 
ATCTCTCAAC CTCCTACCTC AAACTTCCTC AACTGCCTTA AAGGGCTCTT GAGCATGGAG 
AAGATTCATG CCATAATTCG CTTCCTTCCT GTTATCTTCA ACCAGCTCTT TAAAGTGCTT 
ACGCAAAACG ACTCTGATGA AGTCACCACC AGCACCACCA GGGTCCTGAT ACACATCTTA 
GCCAAGTGCC ACGAGGAGAA CCTGGACCAC TATTTGCACT CCTACATTAA GTTTGTCTTT 
AAGACTGAAG CGTGTGGCTT CAGAACCATT CATGAAGAGC TGGCCAAGGG AATGACCTCT 
GACCTGAAGA GCAATGAACA GTTAGTCATA CGCAACGTCC TGAAGTTCTC GTGGTTCTTT 
CTCGAGATCA TAGTGAAGTC AATGGCTCAG CATCTGCTGG ACTCAAACAA GCTCAAGCTG 
CCGCGACCGC AACGATTTCC TGGCACTTTC CAAAACCGTT TGGAGACGTT GATCATGACC 
ATGTCGGATC ACATTTACTG GAAGAACAAA GAGCTGGCGG AGGAGACGCG GAGTGCAAAT 
ATGGCCATTG CGTTTTTTGT GAAGCGGTGC TTCACCTTGA TGGACAGAGG TTTCACCTTT 
AAACTGATCA GCAACTACCT GAACATGATC ACAGCCACTG ACAATAAGGC GCTGTGTGAG 
CTGAAGTTTG AGTTCATAAG GGAAGTGTGT AACTATGAGC ACTTTGTCCC TTTGAGTCTT 
CCCATCCCGT CTGCACGAAT CACAGACAAT GCATCAACAG AAGCCCAAAG TACTAAGGTG 
GACTTCCCAG AGTACAGTTT GACAGAGGAT TTCTGCAGGA AGCATTTTCT GTCTGGATTG 
TTGTTGCGAG AGCTGGGACT CGCTTTGCAG GATGAGCAAG ATCTGAGACA CATCGCTCTG 
GCCTGCCTGA AGAACCTGAT GGCCAAACAC TCGCTGGACG CTCGATACGC CACAAAGGAG 
AAACAGGCTC GGATAGCTTC TCTTTACCTG CCGCTTTATG GCCTGATCCT GGACAACATG 
CCTCGGTTTT TCCTCAGAGA TCTGTTTCCA ATCTGCCTGA CAGCGAGTGA CCAGGGATCC 
AGAGATGATC TCAGTGTTGG AGTAGGTTTG GGTGGTCAGG TGACCCATGT TTTACGCCAT 
GGAAACTCAA TGGACGCGTC CTTCTCCAAG GAAGTGCTCA ACTCCATTAC AGCCTTTTCA 
TCATTGGCTG TATCGACCGC TAATCATGCC GACTCCAGGG GTTCTCTGAT CAGCATCGAG 
TCGAACCCCA GCAACAGTGA CCGCAACAGT GAGAAGATGG ATGCCTGTGA AAAGTTTGCC 
AGGCCTCAGT CTCTTATTGG CTTTGGATCC CGCTTTGATA AACTGGATCA AGCTGAAACC 
AGAAGTCTGC TCATGTGTTT CCTGCACATT ATGAAGACCA TCTCAGAGGA GGTGATGGTT 
TCATACTGGC ATCGAGCGAT TCACCAGGAG ATCAGTGACT TCTTCAATAT TTTGGAATTA 
TGCCTTCAGC ACTTTCGTTT CCTTGGAAAG CGGCACATTG CCAGGATTCT GTCTGCACAG 
GACGGTCACA GACACGCTCG CTCTCAGACC ATGCCCATCA TCAGGGGGAA AAACGCCCTC 
ACAAACCCCA AACTGCTGCA TATGATGGAG ACAGATGGAA ATGTTGCTGA AGGAGAAAAC 
TTTAACCCTA CAGACGTGGA GGCGAACCTT TCTACTGAAG TAGCACTGAC CGTGTTGGAT 
GTTCTGGACC TGTTTGTTCG ACATCATAAG AAACAGTTGC AGCACGATGA AGGACAAAAC 
TCTTTGATGA AGAAAGTGTT CGACACGTAC CTGCTGTTCT TCCAGATCAA CCAGTCCACC 
ACCACCCTCA GACACGTGTT CACAGCCCTG CGCCTCTTCA TGCAGAAGTT TCCGTCCACG 
TTTTTCCAGG GTAAAGCGGA TGTGTGTGGC AGCCTGTGTT ACGAGATCCT GAAGTGCTGT 
AACCATCGCT CTAGCTCCAC ACAGACTGAA GCCGCCGCTC TGCTTTATTT CCTCATGAGG 
AGGAACTTTG AGTTCACGAA GGGCAAGTGC ATCGTCCGCT CACACCTGCA GGTGATTAAA 
GCTGTGAGTC AGCTGATCGC GGATGCAGGC ATTGGCGGAT CACGCTTCCA GCAGTCTCTG 
GCCATCATCA ATAACTTCGC CAATGGGGAC ACGCCACTTA AAAACACCAC ATTCCCAGCT 
GACGTAAAGG ACCTGACCAA GAGGATACGC ACCGTCCTGA TGGCCACGTC TCAAATGAAA 
GAGCATGAAA AAGACCCGGA AATGCTGGTG GACCTTCAGT ACAGTCTGGC CAACTCCTAC 
GCCAGCACAC CTGAGCTCAG ACGCACCTGG CTGGAAAGCA TGGCCAAAAT ACACGTCAGA 
AATGGAGATC TGTCTGAGGC AGCCATGTGC TACATCCACA TCTCAGCCCT CATAGCAGAG 
TCTCTGAGGA GAAGAGGTTA CTGGAAGTCT GAAAAGGGTC GGATGTCAAG TGTGAGCAAA 
GAGGGAGCCT GTGTAAATAA CTGTAGCCCT TTACTAACTC CCCAAGACGG AGAAACATCA 
TTCAGTATGG GTTGGGCGGC ATTTACGAAT ATCTCGCCTA ACGTTAAAGA AGAGGGAGCA 
ATGAAAGAAG ACGCTGGCAC TCAGGATACT CCATACACTG AGGACACGCT GGTGGAGCAG 
CTGGAGATGT GTGTTGATTA TCTCTGGAAG TCTGAGAGAT ATGAACTTAT TGCCGAGATC 
AACAAACCTG TTATTGTCGT CTTTGAGAAG AGAAGAGATT TCAAGCGTCT ATCAGAGCTG 
TACTATGACA TTCACCGCTC ATACCTGAAG GTGACCGAAG TCGTGAATTC TGAGAAACGT 
CTGTTTGGTC GGTACTATCG CGTGGCTTTC TACGGACAGG GGTTTTTTGA GGAAGAGGAG 
AGTAAGGAGT TTATCTACAA GGAGCCCAAA CTCACAGGAC TGTCCGAAAT TTCTCAAAGA 
CTCCTCAAAC TCTACTCGGA CAAGTTTGGA GCTGATAACG TCAAAATGAT CCAGGACTCC 
AACAAGGTGA ATCCGAAAGA CTTGGACCCC AGATTTGCCT ACATCCAGGT CACCTATGTA 
GTGCCTTATT TTAATGAGAA GGAACAGCTG GAGAAGAAAA CCGACTTCGA GCGCCATCAC 
AACATCGACC GATTTGTGTT TGAGACGCCC TTCACGCTTT CTGGAAAAAA ACACGGAGAC 
GTGGAGGAAC AGTGCAAAAG GAAAACCATC CTGACCACGA GCTCTAGTTT CCCTTTCTTG 
AAGAAGCGTA TAGAGGTGGT GGAGCAACAG AGTACCGAGA TGAACCCTAT TGAGGTGGCG 
ATAGACGAGA TGAGCCGAAA AGTGTCTGAA CTCAAACAGC TGTGCAATAT GGAGGAGGTG 
GACATGATCC GTCTGCAGCT CAAACTACAG GGCAGCGTCA GCGTCAAGGT AAATGCAGGA 
CCCATGGCCT ACGCCCGAGC CTTCCTAGAG GAGAAAAATG CTAAGAAATA CCCAGACAAC 
CAGGTCAAAC TGCTGAAAGA GATCTTCAGA CAGTTTGCAG AAGCGTGTGG ACAGGCTCTG 
AGTGTGAACG AGCGGCTAAT AAAAGAGGAT CAGTTTGAGT ATCAGAGCGA GATGAAAGCT 
CACTATAAAG ACATGCTCAC GGAGCTCTCC GCCATTATGA ATGAGCAGAT ACCGTACACG 
AGACAACCAG GAACAGAGCG ACACAGTGCC ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289691.1
CDS	176..6631
Translation	MGCTTSLLQAVNFIMRRNHSRVFSDQQKQHLLETAPLEEGMVISEPHSTVVMQEKPKLIDPLDYEAVISELEAEFREDPLQDLLLFPDLDFTVSTLPVERRTVKSTVPEGADEHAECLLVRQACQYFNSQLHIVQYKYDEYGGDYRMLPRKLCKAEKLPSHSFEIDNEDADKDEDTTSLSSSKGGGGGAGSGGGGGIGVFKSGWLYKGNFNSTVNNSITVRSFKRRYFQLTQLSDNSYIMNFYKDEKISKEPKGCIFLDSCTGVVQNNRLRKHAFELKMNEVTYFVLAAENEQDMDDWINTLNRILQISPPDGPAQDRKSTDMSDSKNEISDIPQNNVPVHKNEETTENEIHPELAKYISETEESSRAVRAEERLNLFSLDPDIPVLRSPRTETGESSSVHPFDEKLGKRLMICCRSLNLTLQGCVNEADTEPTTNIEPFFVSVCLLDVREGRKVSADFHVDVNHELVRQMLNSSGNPGDQGSDVGNGAPLENGLTSPVEKKPEDCQLGPDMEKWLCFPKQAIFSITNPHTDIVMVARVEKVLMGNISSGAEPYIKNTDSSKTVHKMLKSNKQFCSKLGKYRMPFAWSVRSVFKDNQGTVDRESRFSPLFKQESNKISTEDLIKLITEYRRAEKASKLQTIPGNLEINVDCVPMECPNCVTSSYVALKPFEDCSQHAPTVEIDEFLQDSSKFAQPYRVYKNHIYIYPKHLKYDSQKSFAKARNIAVCVEFRSSDEEHAKPLKCIYGKPGGPVFTTTACTTVLHHSQNPDFYDEVKIELPTHLHEKHHILFSFYHVTCDINAKTSSKKKEALELPVGYAWLPLLKDGRISSQDFSIPVSCTLPAGYLHIKEPSSTKNGSDVKWVDGGKQIFKLSTLVLSTVYTQDPHLNRFFQQCQKREAEISQPPTSNFLNCLKGLLSMEKIHAIIRFLPVIFNQLFKVLTQNDSDEVTTSTTRVLIHILAKCHEENLDHYLHSYIKFVFKTEACGFRTIHEELAKGMTSDLKSNEQLVIRNVLKFSWFFLEIIVKSMAQHLLDSNKLKLPRPQRFPGTFQNRLETLIMTMSDHIYWKNKELAEETRSANMAIAFFVKRCFTLMDRGFTFKLISNYLNMITATDNKALCELKFEFIREVCNYEHFVPLSLPIPSARITDNASTEAQSTKVDFPEYSLTEDFCRKHFLSGLLLRELGLALQDEQDLRHIALACLKNLMAKHSLDARYATKEKQARIASLYLPLYGLILDNMPRFFLRDLFPICLTASDQGSRDDLSVGVGLGGQVTHVLRHGNSMDASFSKEVLNSITAFSSLAVSTANHADSRGSLISIESNPSNSDRNSEKMDACEKFARPQSLIGFGSRFDKLDQAETRSLLMCFLHIMKTISEEVMVSYWHRAIHQEISDFFNILELCLQHFRFLGKRHIARILSAQDGHRHARSQTMPIIRGKNALTNPKLLHMMETDGNVAEGENFNPTDVEANLSTEVALTVLDVLDLFVRHHKKQLQHDEGQNSLMKKVFDTYLLFFQINQSTTTLRHVFTALRLFMQKFPSTFFQGKADVCGSLCYEILKCCNHRSSSTQTEAAALLYFLMRRNFEFTKGKCIVRSHLQVIKAVSQLIADAGIGGSRFQQSLAIINNFANGDTPLKNTTFPADVKDLTKRIRTVLMATSQMKEHEKDPEMLVDLQYSLANSYASTPELRRTWLESMAKIHVRNGDLSEAAMCYIHISALIAESLRRRGYWKSEKGRMSSVSKEGACVNNCSPLLTPQDGETSFSMGWAAFTNISPNVKEEGAMKEDAGTQDTPYTEDTLVEQLEMCVDYLWKSERYELIAEINKPVIVVFEKRRDFKRLSELYYDIHRSYLKVTEVVNSEKRLFGRYYRVAFYGQGFFEEEESKEFIYKEPKLTGLSEISQRLLKLYSDKFGADNVKMIQDSNKVNPKDLDPRFAYIQVTYVVPYFNEKEQLEKKTDFERHHNIDRFVFETPFTLSGKKHGDVEEQCKRKTILTTSSSFPFLKKRIEVVEQQSTEMNPIEVAIDEMSRKVSELKQLCNMEEVDMIRLQLKLQGSVSVKVNAGPMAYARAFLEEKNAKKYPDNQVKLLKEIFRQFAEACGQALSVNERLIKEDQFEYQSEMKAHYKDMLTELSAIMNEQIPYTRQPGTERHSAI

Target ORF information:

RefSeq Version	XM_009291416.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio dedicator of cytokinesis 10 (dock10), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291416.2

ORF Insert Sequence:

ATGGGCTGTA CCACGAGTCT ACTACAGGCT GTGAATTTTA TAATGAGGAG AAACCATTCA 
CGCGTCTTTT CTGACCAGCA GAAACAACAT TTGCTGGAAA CTGCACCGCT GGAGGAGGGG 
ATGGTCATCA GTGAACCACA CAGCACTGTC GTCATGCAGG AGAAGCCCAA ACTGATCGAC 
CCTCTGGACT ATGAAGCTGT GATTTCTGAA CTTGAAGCAG AATTCAGAGA AGATCCTCTT 
CAAGATCTCC TGTTATTCCC AGACCTTGAC TTCACTGTGT CCACACTTCC TGTAGAAAGG 
AGAACAGTGA AGTCTACTGT CCCAGAGGGA GCTGATGAAC ATGCTGAATG TCTGCTGGTC 
AGACAGGCCT GCCAATACTT CAACTCCCAG CTGCACATTG TTCAGTATAA ATATGATGAG 
TATGGAGGGG ACTACCGCAT GCTGCCCAGG AAGCTGTGTA AAGCAGAAAA GTTGCCTTCC 
CACTCTTTTG AGATTGACAA TGAAGATGCG GACAAAGATG AGGACACAAC ATCTTTGTCC 
TCATCTAAAG GAGGGGGCGG CGGTGCAGGC AGTGGAGGCG GAGGAGGAAT TGGTGTTTTT 
AAGTCCGGCT GGCTCTATAA AGGCAACTTC AACAGCACCG TCAACAACAG CATCACAGTT 
CGGTCCTTCA AGCGCCGATA TTTTCAGCTG ACTCAGCTCT CTGACAACTC TTACATCATG 
AACTTCTATA AAGATGAGAA AATTTCCAAG GAACCGAAAG GCTGTATTTT TCTGGACTCC 
TGCACCGGGG TGGTTCAGAA TAATCGACTG AGGAAACATG CGTTTGAGCT GAAAATGAAC 
GAAGTGACAT ATTTTGTCCT GGCTGCCGAG AATGAGCAAG ACATGGACGA CTGGATCAAC 
ACTTTAAATC GCATCCTGCA GATCAGCCCT CCAGACGGCC CGGCTCAGGA CCGCAAGAGT 
ACGGACATGA GCGACTCTAA AAACGAGATC AGTGATATAC CACAAAATAA CGTCCCTGTT 
CATAAAAACG AAGAAACAAC TGAAAATGAG ATCCACCCTG AACTAGCCAA GTATATTTCT 
GAGACAGAAG AAAGCTCGAG AGCTGTCAGA GCAGAGGAGA GACTGAACCT GTTCTCCCTG 
GACCCTGATA TTCCTGTTTT AAGGAGTCCT AGGACAGAAA CCGGGGAGTC TTCTTCTGTC 
CATCCATTTG ATGAGAAGCT TGGGAAGAGG CTGATGATCT GCTGTCGCTC ACTGAACCTC 
ACACTGCAAG GCTGCGTTAA TGAAGCGGAC ACCGAACCCA CCACTAATAT CGAGCCTTTC 
TTTGTGTCAG TGTGTCTGCT GGACGTACGT GAGGGCAGGA AGGTTTCCGC TGACTTCCAT 
GTGGACGTAA ACCATGAGTT AGTGCGGCAG ATGCTTAATA GCTCTGGTAA TCCGGGAGAT 
CAAGGCTCGG ATGTTGGTAA TGGTGCACCG CTGGAAAATG GACTCACTTC CCCGGTAGAA 
AAGAAACCAG AAGACTGTCA ACTAGGCCCA GACATGGAAA AGTGGCTATG CTTTCCCAAA 
CAGGCAATCT TTTCCATTAC GAACCCGCAC ACGGATATTG TGATGGTGGC ACGAGTGGAG 
AAAGTTCTGA TGGGAAATAT TTCCAGTGGT GCAGAGCCCT ACATCAAAAA CACCGATTCC 
AGCAAGACTG TACACAAGAT GCTGAAATCC AATAAGCAGT TCTGTAGCAA ACTAGGCAAG 
TACCGAATGC CTTTCGCCTG GTCTGTAAGG TCAGTGTTTA AGGACAACCA GGGCACAGTG 
GACAGGGAGT CTCGCTTCTC TCCTCTTTTC AAACAGGAAA GCAACAAGAT TTCCACCGAA 
GACCTCATCA AGCTCATCAC CGAGTACAGG AGGGCGGAGA AGGCCAGTAA ACTCCAGACC 
ATCCCTGGAA ACCTTGAAAT AAATGTGGAC TGTGTTCCCA TGGAATGTCC CAATTGTGTG 
ACGTCCTCAT ATGTTGCCCT GAAGCCGTTT GAGGACTGCA GCCAACATGC TCCTACGGTG 
GAAATCGATG AGTTCCTGCA GGATTCGTCA AAGTTCGCTC AACCTTACAG AGTCTACAAG 
AACCACATCT ACATCTACCC TAAACACCTC AAGTATGACA GCCAGAAGAG CTTTGCTAAG 
GCTCGAAATA TTGCAGTTTG TGTGGAGTTT CGAAGCTCCG ATGAAGAACA TGCCAAGCCG 
CTTAAGTGCA TCTATGGAAA GCCCGGAGGC CCAGTTTTTA CCACGACTGC CTGTACCACA 
GTTCTGCACC ATTCACAGAA CCCAGACTTT TATGATGAGG TCAAGATTGA GTTGCCCACA 
CATCTTCATG AGAAACACCA TATACTCTTC TCCTTCTATC ATGTGACCTG TGACATCAAT 
GCCAAGACCA GCTCTAAGAA GAAAGAGGCT CTTGAATTGC CAGTGGGTTA TGCATGGCTG 
CCTCTTCTAA AGGATGGACG CATTTCCTCT CAGGACTTCA GCATTCCTGT CTCCTGCACT 
TTGCCTGCTG GCTATTTACA CATTAAAGAG CCTTCGTCCA CCAAGAATGG CTCTGATGTG 
AAGTGGGTCG ATGGAGGGAA GCAGATCTTC AAATTGTCCA CTTTGGTGCT TTCTACAGTG 
TATACTCAGG ATCCCCATCT AAACCGCTTC TTCCAGCAGT GCCAAAAACG AGAGGCAGAA 
ATCTCTCAAC CTCCTACCTC AAACTTCCTC AACTGCCTTA AAGGGCTCTT GAGCATGGAG 
AAGATTCATG CCATAATTCG CTTCCTTCCT GTTATCTTCA ACCAGCTCTT TAAAGTGCTT 
ACGCAAAACG ACTCTGATGA AGTCACCACC AGCACCACCA GGGTCCTGAT ACACATCTTA 
GCCAAGTGCC ACGAGGAGAA CCTGGACCAC TATTTGCACT CCTACATTAA GTTTGTCTTT 
AAGACTGAAG CGTGTGGCTT CAGAACCATT CATGAAGAGC TGGCCAAGGG AATGACCTCT 
GACCTGAAGA GCAATGAACA GTTAGTCATA CGCAACGTCC TGAAGTTCTC GTGGTTCTTT 
CTCGAGATCA TAGTGAAGTC AATGGCTCAG CATCTGCTGG ACTCAAACAA GCTCAAGCTG 
CCGCGACCGC AACGATTTCC TGGCACTTTC CAAAACCGTT TGGAGACGTT GATCATGACC 
ATGTCGGATC ACATTTACTG GAAGAACAAA GAGCTGGCGG AGGAGACGCG GAGTGCAAAT 
ATGGCCATTG CGTTTTTTGT GAAGCGGTGC TTCACCTTGA TGGACAGAGG TTTCACCTTT 
AAACTGATCA GCAACTACCT GAACATGATC ACAGCCACTG ACAATAAGGC GCTGTGTGAG 
CTGAAGTTTG AGTTCATAAG GGAAGTGTGT AACTATGAGC ACTTTGTCCC TTTGAGTCTT 
CCCATCCCGT CTGCACGAAT CACAGACAAT GCATCAACAG AAGCCCAAAG TACTAAGGTG 
GACTTCCCAG AGTACAGTTT GACAGAGGAT TTCTGCAGGA AGCATTTTCT GTCTGGATTG 
TTGTTGCGAG AGCTGGGACT CGCTTTGCAG GATGAGCAAG ATCTGAGACA CATCGCTCTG 
GCCTGCCTGA AGAACCTGAT GGCCAAACAC TCGCTGGACG CTCGATACGC CACAAAGGAG 
AAACAGGCTC GGATAGCTTC TCTTTACCTG CCGCTTTATG GCCTGATCCT GGACAACATG 
CCTCGGTTTT TCCTCAGAGA TCTGTTTCCA ATCTGCCTGA CAGCGAGTGA CCAGGGATCC 
AGAGATGATC TCAGTGTTGG AGTAGGTTTG GGTGGTCAGG TGACCCATGT TTTACGCCAT 
GGAAACTCAA TGGACGCGTC CTTCTCCAAG GAAGTGCTCA ACTCCATTAC AGCCTTTTCA 
TCATTGGCTG TATCGACCGC TAATCATGCC GACTCCAGGG GTTCTCTGAT CAGCATCGAG 
TCGAACCCCA GCAACAGTGA CCGCAACAGT GAGAAGATGG ATGCCTGTGA AAAGTTTGCC 
AGGCCTCAGT CTCTTATTGG CTTTGGATCC CGCTTTGATA AACTGGATCA AGCTGAAACC 
AGAAGTCTGC TCATGTGTTT CCTGCACATT ATGAAGACCA TCTCAGAGGA GGTGATGGTT 
TCATACTGGC ATCGAGCGAT TCACCAGGAG ATCAGTGACT TCTTCAATAT TTTGGAATTA 
TGCCTTCAGC ACTTTCGTTT CCTTGGAAAG CGGCACATTG CCAGGATTCT GTCTGCACAG 
GACGGTCACA GACACGCTCG CTCTCAGACC ATGCCCATCA TCAGGGGGAA AAACGCCCTC 
ACAAACCCCA AACTGCTGCA TATGATGGAG ACAGATGGAA ATGTTGCTGA AGGAGAAAAC 
TTTAACCCTA CAGACGTGGA GGCGAACCTT TCTACTGAAG TAGCACTGAC CGTGTTGGAT 
GTTCTGGACC TGTTTGTTCG ACATCATAAG AAACAGTTGC AGCACGATGA AGGACAAAAC 
TCTTTGATGA AGAAAGTGTT CGACACGTAC CTGCTGTTCT TCCAGATCAA CCAGTCCACC 
ACCACCCTCA GACACGTGTT CACAGCCCTG CGCCTCTTCA TGCAGAAGTT TCCGTCCACG 
TTTTTCCAGG GTAAAGCGGA TGTGTGTGGC AGCCTGTGTT ACGAGATCCT GAAGTGCTGT 
AACCATCGCT CTAGCTCCAC ACAGACTGAA GCCGCCGCTC TGCTTTATTT CCTCATGAGG 
AGGAACTTTG AGTTCACGAA GGGCAAGTGC ATCGTCCGCT CACACCTGCA GGTGATTAAA 
GCTGTGAGTC AGCTGATCGC GGATGCAGGC ATTGGCGGAT CACGCTTCCA GCAGTCTCTG 
GCCATCATCA ATAACTTCGC CAATGGGGAC ACGCCACTTA AAAACACCAC ATTCCCAGCT 
GACGTAAAGG ACCTGACCAA GAGGATACGC ACCGTCCTGA TGGCCACGTC TCAAATGAAA 
GAGCATGAAA AAGACCCGGA AATGCTGGTG GACCTTCAGT ACAGTCTGGC CAACTCCTAC 
GCCAGCACAC CTGAGCTCAG ACGCACCTGG CTGGAAAGCA TGGCCAAAAT ACACGTCAGA 
AATGGAGATC TGTCTGAGGC AGCCATGTGC TACATCCACA TCTCAGCCCT CATAGCAGAG 
TCTCTGAGGA GAAGAGGTTA CTGGAAGTCT GAAAAGGGTC GGATGTCAAG TGTGAGCAAA 
GAGGGAGCCT GTGTAAATAA CTGTAGCCCT TTACTAACTC CCCAAGACGG AGAAACATCA 
TTCAGTATGG GTTGGGCGGC ATTTACGAAT ATCTCGCCTA ACGTTAAAGA AGAGGGAGCA 
ATGAAAGAAG ACGCTGGCAC TCAGGATACT CCATACACTG AGGACACGCT GGTGGAGCAG 
CTGGAGATGT GTGTTGATTA TCTCTGGAAG TCTGAGAGAT ATGAACTTAT TGCCGAGATC 
AACAAACCTG TTATTGTCGT CTTTGAGAAG AGAAGAGATT TCAAGCGTCT ATCAGAGCTG 
TACTATGACA TTCACCGCTC ATACCTGAAG GTGACCGAAG TCGTGAATTC TGAGAAACGT 
CTGTTTGGTC GGTACTATCG CGTGGCTTTC TACGGACAGG GGTTTTTTGA GGAAGAGGAG 
AGTAAGGAGT TTATCTACAA GGAGCCCAAA CTCACAGGAC TGTCCGAAAT TTCTCAAAGA 
CTCCTCAAAC TCTACTCGGA CAAGTTTGGA GCTGATAACG TCAAAATGAT CCAGGACTCC 
AACAAGGTGA ATCCGAAAGA CTTGGACCCC AGATTTGCCT ACATCCAGGT CACCTATGTA 
GTGCCTTATT TTAATGAGAA GGAACAGCTG GAGAAGAAAA CCGACTTCGA GCGCCATCAC 
AACATCGACC GATTTGTGTT TGAGACGCCC TTCACGCTTT CTGGAAAAAA ACACGGAGAC 
GTGGAGGAAC AGTGCAAAAG GAAAACCATC CTGACCACGA GCTCTAGTTT CCCTTTCTTG 
AAGAAGCGTA TAGAGGTGGT GGAGCAACAG AGTACCGAGA TGAACCCTAT TGAGGTGGCG 
ATAGACGAGA TGAGCCGAAA AGTGTCTGAA CTCAAACAGC TGTGCAATAT GGAGGAGGTG 
GACATGATCC GTCTGCAGCT CAAACTACAG GGCAGCGTCA GCGTCAAGGT AAATGCAGGA 
CCCATGGCCT ACGCCCGAGC CTTCCTAGAG GAGAAAAATG CTAAGAAATA CCCAGACAAC 
CAGGTCAAAC TGCTGAAAGA GATCTTCAGA CAGTTTGCAG AAGCGTGTGG ACAGGCTCTG 
AGTGTGAACG AGCGGCTAAT AAAAGAGGAT CAGTTTGAGT ATCAGAGCGA GATGAAAGCT 
CACTATAAAG ACATGCTCAC GGAGCTCTCC GCCATTATGA ATGAGCAGAT ACCGTACACG 
AGACAACCAG GAACAGAGCG ACACAGTGCC ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOCK10 ( XM_021466297.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021321972.1 Danio rerio dedicator of cytokinesis 10 (dock10), transcript variant X2, mRNA.

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CloneID	ODa149005
Clone ID Related Accession (Same CDS sequence)	XM_021466297.1
Accession Version	XM_021466297.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	dedicator of cytokinesis protein 10 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG AATCGACCCG GAGATTTACG AAGAGCCTGC TGAAGCCGGG GAGCGCAGCG 
GAGATTCGGC AAACCGCTTC CAGCGCCGTG AGGAACGCTA CTATAACGCA GGAGAAGCCC 
AAACTGATCG ACCCTCTGGA CTATGAAGCT GTGATTTCTG AACTTGAAGC AGAATTCAGA 
GAAGATCCTC TTCAAGATCT CCTGTTATTC CCAGACCTTG ACTTCACTGT GTCCACACTT 
CCTGTAGAAA GGAGAACAGT GAAGTCTACT GTCCCAGAGG GAGCTGATGA ACATGCTGAA 
TGTCTGCTGG TCAGACAGGC CTGCCAATAC TTCAACTCCC AGCTGCACAT TGTTCAGTAT 
AAATATGATG AGTATGGAGG GGACTACCGC ATGCTGCCCA GGAAGCTGTG TAAAGCAGAA 
AAGTTGCCTT CCCACTCTTT TGAGATTGAC AATGAAGATG CGGACAAAGA TGAGGACACA 
ACATCTTTGT CCTCATCTAA AGGAGGGGGC GGCGGTGCAG GCAGTGGAGG CGGAGGAGGA 
ATTGGTGTTT TTAAGTCCGG CTGGCTCTAT AAAGGCAACT TCAACAGCAC CGTCAACAAC 
AGCATCACAG TTCGGTCCTT CAAGCGCCGA TATTTTCAGC TGACTCAGCT CTCTGACAAC 
TCTTACATCA TGAACTTCTA TAAAGATGAG AAAATTTCCA AGGAACCGAA AGGCTGTATT 
TTTCTGGACT CCTGCACCGG GGTGGTTCAG AATAATCGAC TGAGGAAACA TGCGTTTGAG 
CTGAAAATGA ACGAAGTGAC ATATTTTGTC CTGGCTGCCG AGAATGAGCA AGACATGGAC 
GACTGGATCA ACACTTTAAA TCGCATCCTG CAGATCAGCC CTCCAGACGG CCCGGCTCAG 
GACCGCAAGA GTACGGACAT GAGCGACTCT AAAAACGAGA TCAGTGATAT ACCACAAAAT 
AACGTCCCTG TTCATAAAAA CGAAGAAACA ACTGAAAATG AGATCCACCC TGAACTAGCC 
AAGTATATTT CTGAGACAGA AGAAAGCTCG AGAGCTGTCA GAGCAGAGGA GAGACTGAAC 
CTGTTCTCCC TGGACCCTGA TATTCCTGTT TTAAGGAGTC CTAGGACAGA AACCGGGGAG 
TCTTCTTCTG TCCATCCATT TGATGAGAAG CTTGGGAAGA GGCTGATGAT CTGCTGTCGC 
TCACTGAACC TCACACTGCA AGGCTGCGTT AATGAAGCGG ACACCGAACC CACCACTAAT 
ATCGAGCCTT TCTTTGTGTC AGTGTGTCTG CTGGACGTAC GTGAGGGCAG GAAGGTTTCC 
GCTGACTTCC ATGTGGACGT AAACCATGAG TTAGTGCGGC AGATGCTTAA TAGCTCTGGT 
AATCCGGGAG ATCAAGGCTC GGATGTTGGT AATGGTGCAC CGCTGGAAAA TGGACTCACT 
TCCCCGGTAG AAAAGAAACC AGAAGACTGT CAACTAGGCC CAGACATGGA AAAGTGGCTA 
TGCTTTCCCA AACAGGCAAT CTTTTCCATT ACGAACCCGC ACACGGATAT TGTGATGGTG 
GCACGAGTGG AGAAAGTTCT GATGGGAAAT ATTTCCAGTG GTGCAGAGCC CTACATCAAA 
AACACCGATT CCAGCAAGAC TGTACACAAG ATGCTGAAAT CCAATAAGCA GTTCTGTAGC 
AAACTAGGCA AGTACCGAAT GCCTTTCGCC TGGTCTGTAA GGTCAGTGTT TAAGGACAAC 
CAGGGCACAG TGGACAGGGA GTCTCGCTTC TCTCCTCTTT TCAAACAGGA AAGCAACAAG 
ATTTCCACCG AAGACCTCAT CAAGCTCATC ACCGAGTACA GGAGGGCGGA GAAGGCCAGT 
AAACTCCAGA CCATCCCTGG AAACCTTGAA ATAAATGTGG ACTGTGTTCC CATGGAATGT 
CCCAATTGTG TGACGTCCTC ATATGTTGCC CTGAAGCCGT TTGAGGACTG CAGCCAACAT 
GCTCCTACGG TGGAAATCGA TGAGTTCCTG CAGGATTCGT CAAAGTTCGC TCAACCTTAC 
AGAGTCTACA AGAACCACAT CTACATCTAC CCTAAACACC TCAAGTATGA CAGCCAGAAG 
AGCTTTGCTA AGGCTCGAAA TATTGCAGTT TGTGTGGAGT TTCGAAGCTC CGATGAAGAA 
CATGCCAAGC CGCTTAAGTG CATCTATGGA AAGCCCGGAG GCCCAGTTTT TACCACGACT 
GCCTGTACCA CAGTTCTGCA CCATTCACAG AACCCAGACT TTTATGATGA GGTCAAGATT 
GAGTTGCCCA CACATCTTCA TGAGAAACAC CATATACTCT TCTCCTTCTA TCATGTGACC 
TGTGACATCA ATGCCAAGAC CAGCTCTAAG AAGAAAGAGG CTCTTGAATT GCCAGTGGGT 
TATGCATGGC TGCCTCTTCT AAAGGATGGA CGCATTTCCT CTCAGGACTT CAGCATTCCT 
GTCTCCTGCA CTTTGCCTGC TGGCTATTTA CACATTAAAG AGCCTTCGTC CACCAAGAAT 
GGCTCTGATG TGAAGTGGGT CGATGGAGGG AAGCAGATCT TCAAATTGTC CACTTTGGTG 
CTTTCTACAG TGTATACTCA GGATCCCCAT CTAAACCGCT TCTTCCAGCA GTGCCAAAAA 
CGAGAGGCAG AAATCTCTCA ACCTCCTACC TCAAACTTCC TCAACTGCCT TAAAGGGCTC 
TTGAGCATGG AGAAGATTCA TGCCATAATT CGCTTCCTTC CTGTTATCTT CAACCAGCTC 
TTTAAAGTGC TTACGCAAAA CGACTCTGAT GAAGTCACCA CCAGCACCAC CAGGGTCCTG 
ATACACATCT TAGCCAAGTG CCACGAGGAG AACCTGGACC ACTATTTGCA CTCCTACATT 
AAGTTTGTCT TTAAGACTGA AGCGTGTGGC TTCAGAACCA TTCATGAAGA GCTGGCCAAG 
GGAATGACCT CTGACCTGAA GAGCAATGAA CAGTTAGTCA TACGCAACGT CCTGAAGTTC 
TCGTGGTTCT TTCTCGAGAT CATAGTGAAG TCAATGGCTC AGCATCTGCT GGACTCAAAC 
AAGCTCAAGC TGCCGCGACC GCAACGATTT CCTGGCACTT TCCAAAACCG TTTGGAGACG 
TTGATCATGA CCATGTCGGA TCACATTTAC TGGAAGAACA AAGAGCTGGC GGAGGAGACG 
CGGAGTGCAA ATATGGCCAT TGCGTTTTTT GTGAAGCGGT GCTTCACCTT GATGGACAGA 
GGTTTCACCT TTAAACTGAT CAGCAACTAC CTGAACATGA TCACAGCCAC TGACAATAAG 
GCGCTGTGTG AGCTGAAGTT TGAGTTCATA AGGGAAGTGT GTAACTATGA GCACTTTGTC 
CCTTTGAGTC TTCCCATCCC GTCTGCACGA ATCACAGACA ATGCATCAAC AGAAGCCCAA 
AGTACTAAGG TGGACTTCCC AGAGTACAGT TTGACAGAGG ATTTCTGCAG GAAGCATTTT 
CTGTCTGGAT TGTTGTTGCG AGAGCTGGGA CTCGCTTTGC AGGATGAGCA AGATCTGAGA 
CACATCGCTC TGGCCTGCCT GAAGAACCTG ATGGCCAAAC ACTCGCTGGA CGCTCGATAC 
GCCACAAAGG AGAAACAGGC TCGGATAGCT TCTCTTTACC TGCCGCTTTA TGGCCTGATC 
CTGGACAACA TGCCTCGGTT TTTCCTCAGA GATCTGTTTC CAATCTGCCT GACAGCGAGT 
GACCAGGGAT CCAGAGATGA TCTCAGTGTT GGAGTAGGTT TGGGTGGTCA GGTGACCCAT 
GTTTTACGCC ATGGAAACTC AATGGACGCG TCCTTCTCCA AGGAAGTGCT CAACTCCATT 
ACAGCCTTTT CATCATTGGC TGTATCGACC GCTAATCATG CCGACTCCAG GGGTTCTCTG 
ATCAGCATCG AGTCGAACCC CAGCAACAGT GACCGCAACA GTGAGAAGAT GGATGCCTGT 
GAAAAGTTTG CCAGGCCTCA GTCTCTTATT GGCTTTGGAT CCCGCTTTGA TAAACTGGAT 
CAAGCTGAAA CCAGAAGTCT GCTCATGTGT TTCCTGCACA TTATGAAGAC CATCTCAGAG 
GAGGTGATGG TTTCATACTG GCATCGAGCG ATTCACCAGG AGATCAGTGA CTTCTTCAAT 
ATTTTGGAAT TATGCCTTCA GCACTTTCGT TTCCTTGGAA AGCGGCACAT TGCCAGGAAG 
CTAGCGGCCG CAGTTAAGCT GGCTCAGGCC ACACAGAACA ATGGCACCCT GAAGGGCTCT 
AATGTGTCCT ACCACTCTCC AGGGCTCCTA CCCCAATGGA TTCTGTCTGC ACAGGACGGT 
CACAGACACG CTCGCTCTCA GACCATGCCC ATCATCAGGG GGAAAAACGC CCTCACAAAC 
CCCAAACTGC TGCATATGAT GGAGACAGAT GGAAATGTTG CTGAAGGAGA AAACTTTAAC 
CCTACAGACG TGGAGGCGAA CCTTTCTACT GAAGTAGCAC TGACCGTGTT GGATGTTCTG 
GACCTGTTTG TTCGACATCA TAAGAAACAG TTGCAGCACG ATGAAGGACA AAACTCTTTG 
ATGAAGAAAG TGTTCGACAC GTACCTGCTG TTCTTCCAGA TCAACCAGTC CACCACCACC 
CTCAGACACG TGTTCACAGC CCTGCGCCTC TTCATGCAGA AGTTTCCGTC CACGTTTTTC 
CAGGGTAAAG CGGATGTGTG TGGCAGCCTG TGTTACGAGA TCCTGAAGTG CTGTAACCAT 
CGCTCTAGCT CCACACAGAC TGAAGCCGCC GCTCTGCTTT ATTTCCTCAT GAGGAGGAAC 
TTTGAGTTCA CGAAGGGCAA GTGCATCGTC CGCTCACACC TGCAGGTGAT TAAAGCTGTG 
AGTCAGCTGA TCGCGGATGC AGGCATTGGC GGATCACGCT TCCAGCAGTC TCTGGCCATC 
ATCAATAACT TCGCCAATGG GGACACGCCA CTTAAAAACA CCACATTCCC AGCTGACGTA 
AAGGACCTGA CCAAGAGGAT ACGCACCGTC CTGATGGCCA CGTCTCAAAT GAAAGAGCAT 
GAAAAAGACC CGGAAATGCT GGTGGACCTT CAGTACAGTC TGGCCAACTC CTACGCCAGC 
ACACCTGAGC TCAGACGCAC CTGGCTGGAA AGCATGGCCA AAATACACGT CAGAAATGGA 
GATCTGTCTG AGGCAGCCAT GTGCTACATC CACATCTCAG CCCTCATAGC AGAGTCTCTG 
AGGAGAAGAG GTTACTGGAA GTCTGAAAAG GGTCGGATGT CAAGTGTGAG CAAAGAGGGA 
GCCTGTGTAA ATAACTGTAG CCCTTTACTA ACTCCCCAAG ACGGAGAAAC ATCATTCAGT 
ATGGGTTGGG CGGCATTTAC GAATATCTCG CCTAACGTTA AAGAAGAGGG AGCAATGAAA 
GAAGACGCTG GCACTCAGGA TACTCCATAC ACTGAGGACA CGCTGGTGGA GCAGCTGGAG 
ATGTGTGTTG ATTATCTCTG GAAGTCTGAG AGATATGAAC TTATTGCCGA GATCAACAAA 
CCTGTTATTG TCGTCTTTGA GAAGAGAAGA GATTTCAAGC GTCTATCAGA GCTGTACTAT 
GACATTCACC GCTCATACCT GAAGGTGACC GAAGTCGTGA ATTCTGAGAA ACGTCTGTTT 
GGTCGGTACT ATCGCGTGGC TTTCTACGGA CAGGCAGTGG GGTTTTTTGA GGAAGAGGAG 
AGTAAGGAGT TTATCTACAA GGAGCCCAAA CTCACAGGAC TGTCCGAAAT TTCTCAAAGA 
CTCCTCAAAC TCTACTCGGA CAAGTTTGGA GCTGATAACG TCAAAATGAT CCAGGACTCC 
AACAAGGTGA ATCCGAAAGA CTTGGACCCC AGATTTGCCT ACATCCAGGT CACCTATGTA 
GTGCCTTATT TTAATGAGAA GGAACAGCTG GAGAAGAAAA CCGACTTCGA GCGCCATCAC 
AACATCGACC GATTTGTGTT TGAGACGCCC TTCACGCTTT CTGGAAAAAA ACACGGAGAC 
GTGGAGGAAC AGTGCAAAAG GAAAACCATC CTGACCACGA GCTCTAGTTT CCCTTTCTTG 
AAGAAGCGTA TAGAGGTGGT GGAGCAACAG AGTACCGAGA TGAACCCTAT TGAGGTGGCG 
ATAGACGAGA TGAGCCGAAA AGTGTCTGAA CTCAAACAGC TGTGCAATAT GGAGGAGGTG 
GACATGATCC GTCTGCAGCT CAAACTACAG GGCAGCGTCA GCGTCAAGGT AAATGCAGGA 
CCCATGGCCT ACGCCCGAGC CTTCCTAGAG GAGAAAAATG CTAAGAAATA CCCAGACAAC 
CAGGTCAAAC TGCTGAAAGA GATCTTCAGA CAGTTTGCAG AAGCGTGTGG ACAGGCTCTG 
AGTGTGAACG AGCGGCTAAT AAAAGAGGAT CAGTTTGAGT ATCAGAGCGA GATGAAAGCT 
CACTATAAAG ACATGCTCAC GGAGCTCTCC GCCATTATGA ATGAGCAGAT ACCGTACACG 
AGACAACCAG GAACAGAGCG ACACAGTGCC ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021321972.1
CDS	161..6676
Translation	MAAESTRRFTKSLLKPGSAAEIRQTASSAVRNATITQEKPKLIDPLDYEAVISELEAEFREDPLQDLLLFPDLDFTVSTLPVERRTVKSTVPEGADEHAECLLVRQACQYFNSQLHIVQYKYDEYGGDYRMLPRKLCKAEKLPSHSFEIDNEDADKDEDTTSLSSSKGGGGGAGSGGGGGIGVFKSGWLYKGNFNSTVNNSITVRSFKRRYFQLTQLSDNSYIMNFYKDEKISKEPKGCIFLDSCTGVVQNNRLRKHAFELKMNEVTYFVLAAENEQDMDDWINTLNRILQISPPDGPAQDRKSTDMSDSKNEISDIPQNNVPVHKNEETTENEIHPELAKYISETEESSRAVRAEERLNLFSLDPDIPVLRSPRTETGESSSVHPFDEKLGKRLMICCRSLNLTLQGCVNEADTEPTTNIEPFFVSVCLLDVREGRKVSADFHVDVNHELVRQMLNSSGNPGDQGSDVGNGAPLENGLTSPVEKKPEDCQLGPDMEKWLCFPKQAIFSITNPHTDIVMVARVEKVLMGNISSGAEPYIKNTDSSKTVHKMLKSNKQFCSKLGKYRMPFAWSVRSVFKDNQGTVDRESRFSPLFKQESNKISTEDLIKLITEYRRAEKASKLQTIPGNLEINVDCVPMECPNCVTSSYVALKPFEDCSQHAPTVEIDEFLQDSSKFAQPYRVYKNHIYIYPKHLKYDSQKSFAKARNIAVCVEFRSSDEEHAKPLKCIYGKPGGPVFTTTACTTVLHHSQNPDFYDEVKIELPTHLHEKHHILFSFYHVTCDINAKTSSKKKEALELPVGYAWLPLLKDGRISSQDFSIPVSCTLPAGYLHIKEPSSTKNGSDVKWVDGGKQIFKLSTLVLSTVYTQDPHLNRFFQQCQKREAEISQPPTSNFLNCLKGLLSMEKIHAIIRFLPVIFNQLFKVLTQNDSDEVTTSTTRVLIHILAKCHEENLDHYLHSYIKFVFKTEACGFRTIHEELAKGMTSDLKSNEQLVIRNVLKFSWFFLEIIVKSMAQHLLDSNKLKLPRPQRFPGTFQNRLETLIMTMSDHIYWKNKELAEETRSANMAIAFFVKRCFTLMDRGFTFKLISNYLNMITATDNKALCELKFEFIREVCNYEHFVPLSLPIPSARITDNASTEAQSTKVDFPEYSLTEDFCRKHFLSGLLLRELGLALQDEQDLRHIALACLKNLMAKHSLDARYATKEKQARIASLYLPLYGLILDNMPRFFLRDLFPICLTASDQGSRDDLSVGVGLGGQVTHVLRHGNSMDASFSKEVLNSITAFSSLAVSTANHADSRGSLISIESNPSNSDRNSEKMDACEKFARPQSLIGFGSRFDKLDQAETRSLLMCFLHIMKTISEEVMVSYWHRAIHQEISDFFNILELCLQHFRFLGKRHIARKLAAAVKLAQATQNNGTLKGSNVSYHSPGLLPQWILSAQDGHRHARSQTMPIIRGKNALTNPKLLHMMETDGNVAEGENFNPTDVEANLSTEVALTVLDVLDLFVRHHKKQLQHDEGQNSLMKKVFDTYLLFFQINQSTTTLRHVFTALRLFMQKFPSTFFQGKADVCGSLCYEILKCCNHRSSSTQTEAAALLYFLMRRNFEFTKGKCIVRSHLQVIKAVSQLIADAGIGGSRFQQSLAIINNFANGDTPLKNTTFPADVKDLTKRIRTVLMATSQMKEHEKDPEMLVDLQYSLANSYASTPELRRTWLESMAKIHVRNGDLSEAAMCYIHISALIAESLRRRGYWKSEKGRMSSVSKEGACVNNCSPLLTPQDGETSFSMGWAAFTNISPNVKEEGAMKEDAGTQDTPYTEDTLVEQLEMCVDYLWKSERYELIAEINKPVIVVFEKRRDFKRLSELYYDIHRSYLKVTEVVNSEKRLFGRYYRVAFYGQAVGFFEEEESKEFIYKEPKLTGLSEISQRLLKLYSDKFGADNVKMIQDSNKVNPKDLDPRFAYIQVTYVVPYFNEKEQLEKKTDFERHHNIDRFVFETPFTLSGKKHGDVEEQCKRKTILTTSSSFPFLKKRIEVVEQQSTEMNPIEVAIDEMSRKVSELKQLCNMEEVDMIRLQLKLQGSVSVKVNAGPMAYARAFLEEKNAKKYPDNQVKLLKEIFRQFAEACGQALSVNERLIKEDQFEYQSEMKAHYKDMLTELSAIMNEQIPYTRQPGTERHSAI

Target ORF information:

RefSeq Version	XM_021466297.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio dedicator of cytokinesis 10 (dock10), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021466297.1

ORF Insert Sequence:

ATGGCAGCGG AATCGACCCG GAGATTTACG AAGAGCCTGC TGAAGCCGGG GAGCGCAGCG 
GAGATTCGGC AAACCGCTTC CAGCGCCGTG AGGAACGCTA CTATAACGCA GGAGAAGCCC 
AAACTGATCG ACCCTCTGGA CTATGAAGCT GTGATTTCTG AACTTGAAGC AGAATTCAGA 
GAAGATCCTC TTCAAGATCT CCTGTTATTC CCAGACCTTG ACTTCACTGT GTCCACACTT 
CCTGTAGAAA GGAGAACAGT GAAGTCTACT GTCCCAGAGG GAGCTGATGA ACATGCTGAA 
TGTCTGCTGG TCAGACAGGC CTGCCAATAC TTCAACTCCC AGCTGCACAT TGTTCAGTAT 
AAATATGATG AGTATGGAGG GGACTACCGC ATGCTGCCCA GGAAGCTGTG TAAAGCAGAA 
AAGTTGCCTT CCCACTCTTT TGAGATTGAC AATGAAGATG CGGACAAAGA TGAGGACACA 
ACATCTTTGT CCTCATCTAA AGGAGGGGGC GGCGGTGCAG GCAGTGGAGG CGGAGGAGGA 
ATTGGTGTTT TTAAGTCCGG CTGGCTCTAT AAAGGCAACT TCAACAGCAC CGTCAACAAC 
AGCATCACAG TTCGGTCCTT CAAGCGCCGA TATTTTCAGC TGACTCAGCT CTCTGACAAC 
TCTTACATCA TGAACTTCTA TAAAGATGAG AAAATTTCCA AGGAACCGAA AGGCTGTATT 
TTTCTGGACT CCTGCACCGG GGTGGTTCAG AATAATCGAC TGAGGAAACA TGCGTTTGAG 
CTGAAAATGA ACGAAGTGAC ATATTTTGTC CTGGCTGCCG AGAATGAGCA AGACATGGAC 
GACTGGATCA ACACTTTAAA TCGCATCCTG CAGATCAGCC CTCCAGACGG CCCGGCTCAG 
GACCGCAAGA GTACGGACAT GAGCGACTCT AAAAACGAGA TCAGTGATAT ACCACAAAAT 
AACGTCCCTG TTCATAAAAA CGAAGAAACA ACTGAAAATG AGATCCACCC TGAACTAGCC 
AAGTATATTT CTGAGACAGA AGAAAGCTCG AGAGCTGTCA GAGCAGAGGA GAGACTGAAC 
CTGTTCTCCC TGGACCCTGA TATTCCTGTT TTAAGGAGTC CTAGGACAGA AACCGGGGAG 
TCTTCTTCTG TCCATCCATT TGATGAGAAG CTTGGGAAGA GGCTGATGAT CTGCTGTCGC 
TCACTGAACC TCACACTGCA AGGCTGCGTT AATGAAGCGG ACACCGAACC CACCACTAAT 
ATCGAGCCTT TCTTTGTGTC AGTGTGTCTG CTGGACGTAC GTGAGGGCAG GAAGGTTTCC 
GCTGACTTCC ATGTGGACGT AAACCATGAG TTAGTGCGGC AGATGCTTAA TAGCTCTGGT 
AATCCGGGAG ATCAAGGCTC GGATGTTGGT AATGGTGCAC CGCTGGAAAA TGGACTCACT 
TCCCCGGTAG AAAAGAAACC AGAAGACTGT CAACTAGGCC CAGACATGGA AAAGTGGCTA 
TGCTTTCCCA AACAGGCAAT CTTTTCCATT ACGAACCCGC ACACGGATAT TGTGATGGTG 
GCACGAGTGG AGAAAGTTCT GATGGGAAAT ATTTCCAGTG GTGCAGAGCC CTACATCAAA 
AACACCGATT CCAGCAAGAC TGTACACAAG ATGCTGAAAT CCAATAAGCA GTTCTGTAGC 
AAACTAGGCA AGTACCGAAT GCCTTTCGCC TGGTCTGTAA GGTCAGTGTT TAAGGACAAC 
CAGGGCACAG TGGACAGGGA GTCTCGCTTC TCTCCTCTTT TCAAACAGGA AAGCAACAAG 
ATTTCCACCG AAGACCTCAT CAAGCTCATC ACCGAGTACA GGAGGGCGGA GAAGGCCAGT 
AAACTCCAGA CCATCCCTGG AAACCTTGAA ATAAATGTGG ACTGTGTTCC CATGGAATGT 
CCCAATTGTG TGACGTCCTC ATATGTTGCC CTGAAGCCGT TTGAGGACTG CAGCCAACAT 
GCTCCTACGG TGGAAATCGA TGAGTTCCTG CAGGATTCGT CAAAGTTCGC TCAACCTTAC 
AGAGTCTACA AGAACCACAT CTACATCTAC CCTAAACACC TCAAGTATGA CAGCCAGAAG 
AGCTTTGCTA AGGCTCGAAA TATTGCAGTT TGTGTGGAGT TTCGAAGCTC CGATGAAGAA 
CATGCCAAGC CGCTTAAGTG CATCTATGGA AAGCCCGGAG GCCCAGTTTT TACCACGACT 
GCCTGTACCA CAGTTCTGCA CCATTCACAG AACCCAGACT TTTATGATGA GGTCAAGATT 
GAGTTGCCCA CACATCTTCA TGAGAAACAC CATATACTCT TCTCCTTCTA TCATGTGACC 
TGTGACATCA ATGCCAAGAC CAGCTCTAAG AAGAAAGAGG CTCTTGAATT GCCAGTGGGT 
TATGCATGGC TGCCTCTTCT AAAGGATGGA CGCATTTCCT CTCAGGACTT CAGCATTCCT 
GTCTCCTGCA CTTTGCCTGC TGGCTATTTA CACATTAAAG AGCCTTCGTC CACCAAGAAT 
GGCTCTGATG TGAAGTGGGT CGATGGAGGG AAGCAGATCT TCAAATTGTC CACTTTGGTG 
CTTTCTACAG TGTATACTCA GGATCCCCAT CTAAACCGCT TCTTCCAGCA GTGCCAAAAA 
CGAGAGGCAG AAATCTCTCA ACCTCCTACC TCAAACTTCC TCAACTGCCT TAAAGGGCTC 
TTGAGCATGG AGAAGATTCA TGCCATAATT CGCTTCCTTC CTGTTATCTT CAACCAGCTC 
TTTAAAGTGC TTACGCAAAA CGACTCTGAT GAAGTCACCA CCAGCACCAC CAGGGTCCTG 
ATACACATCT TAGCCAAGTG CCACGAGGAG AACCTGGACC ACTATTTGCA CTCCTACATT 
AAGTTTGTCT TTAAGACTGA AGCGTGTGGC TTCAGAACCA TTCATGAAGA GCTGGCCAAG 
GGAATGACCT CTGACCTGAA GAGCAATGAA CAGTTAGTCA TACGCAACGT CCTGAAGTTC 
TCGTGGTTCT TTCTCGAGAT CATAGTGAAG TCAATGGCTC AGCATCTGCT GGACTCAAAC 
AAGCTCAAGC TGCCGCGACC GCAACGATTT CCTGGCACTT TCCAAAACCG TTTGGAGACG 
TTGATCATGA CCATGTCGGA TCACATTTAC TGGAAGAACA AAGAGCTGGC GGAGGAGACG 
CGGAGTGCAA ATATGGCCAT TGCGTTTTTT GTGAAGCGGT GCTTCACCTT GATGGACAGA 
GGTTTCACCT TTAAACTGAT CAGCAACTAC CTGAACATGA TCACAGCCAC TGACAATAAG 
GCGCTGTGTG AGCTGAAGTT TGAGTTCATA AGGGAAGTGT GTAACTATGA GCACTTTGTC 
CCTTTGAGTC TTCCCATCCC GTCTGCACGA ATCACAGACA ATGCATCAAC AGAAGCCCAA 
AGTACTAAGG TGGACTTCCC AGAGTACAGT TTGACAGAGG ATTTCTGCAG GAAGCATTTT 
CTGTCTGGAT TGTTGTTGCG AGAGCTGGGA CTCGCTTTGC AGGATGAGCA AGATCTGAGA 
CACATCGCTC TGGCCTGCCT GAAGAACCTG ATGGCCAAAC ACTCGCTGGA CGCTCGATAC 
GCCACAAAGG AGAAACAGGC TCGGATAGCT TCTCTTTACC TGCCGCTTTA TGGCCTGATC 
CTGGACAACA TGCCTCGGTT TTTCCTCAGA GATCTGTTTC CAATCTGCCT GACAGCGAGT 
GACCAGGGAT CCAGAGATGA TCTCAGTGTT GGAGTAGGTT TGGGTGGTCA GGTGACCCAT 
GTTTTACGCC ATGGAAACTC AATGGACGCG TCCTTCTCCA AGGAAGTGCT CAACTCCATT 
ACAGCCTTTT CATCATTGGC TGTATCGACC GCTAATCATG CCGACTCCAG GGGTTCTCTG 
ATCAGCATCG AGTCGAACCC CAGCAACAGT GACCGCAACA GTGAGAAGAT GGATGCCTGT 
GAAAAGTTTG CCAGGCCTCA GTCTCTTATT GGCTTTGGAT CCCGCTTTGA TAAACTGGAT 
CAAGCTGAAA CCAGAAGTCT GCTCATGTGT TTCCTGCACA TTATGAAGAC CATCTCAGAG 
GAGGTGATGG TTTCATACTG GCATCGAGCG ATTCACCAGG AGATCAGTGA CTTCTTCAAT 
ATTTTGGAAT TATGCCTTCA GCACTTTCGT TTCCTTGGAA AGCGGCACAT TGCCAGGAAG 
CTAGCGGCCG CAGTTAAGCT GGCTCAGGCC ACACAGAACA ATGGCACCCT GAAGGGCTCT 
AATGTGTCCT ACCACTCTCC AGGGCTCCTA CCCCAATGGA TTCTGTCTGC ACAGGACGGT 
CACAGACACG CTCGCTCTCA GACCATGCCC ATCATCAGGG GGAAAAACGC CCTCACAAAC 
CCCAAACTGC TGCATATGAT GGAGACAGAT GGAAATGTTG CTGAAGGAGA AAACTTTAAC 
CCTACAGACG TGGAGGCGAA CCTTTCTACT GAAGTAGCAC TGACCGTGTT GGATGTTCTG 
GACCTGTTTG TTCGACATCA TAAGAAACAG TTGCAGCACG ATGAAGGACA AAACTCTTTG 
ATGAAGAAAG TGTTCGACAC GTACCTGCTG TTCTTCCAGA TCAACCAGTC CACCACCACC 
CTCAGACACG TGTTCACAGC CCTGCGCCTC TTCATGCAGA AGTTTCCGTC CACGTTTTTC 
CAGGGTAAAG CGGATGTGTG TGGCAGCCTG TGTTACGAGA TCCTGAAGTG CTGTAACCAT 
CGCTCTAGCT CCACACAGAC TGAAGCCGCC GCTCTGCTTT ATTTCCTCAT GAGGAGGAAC 
TTTGAGTTCA CGAAGGGCAA GTGCATCGTC CGCTCACACC TGCAGGTGAT TAAAGCTGTG 
AGTCAGCTGA TCGCGGATGC AGGCATTGGC GGATCACGCT TCCAGCAGTC TCTGGCCATC 
ATCAATAACT TCGCCAATGG GGACACGCCA CTTAAAAACA CCACATTCCC AGCTGACGTA 
AAGGACCTGA CCAAGAGGAT ACGCACCGTC CTGATGGCCA CGTCTCAAAT GAAAGAGCAT 
GAAAAAGACC CGGAAATGCT GGTGGACCTT CAGTACAGTC TGGCCAACTC CTACGCCAGC 
ACACCTGAGC TCAGACGCAC CTGGCTGGAA AGCATGGCCA AAATACACGT CAGAAATGGA 
GATCTGTCTG AGGCAGCCAT GTGCTACATC CACATCTCAG CCCTCATAGC AGAGTCTCTG 
AGGAGAAGAG GTTACTGGAA GTCTGAAAAG GGTCGGATGT CAAGTGTGAG CAAAGAGGGA 
GCCTGTGTAA ATAACTGTAG CCCTTTACTA ACTCCCCAAG ACGGAGAAAC ATCATTCAGT 
ATGGGTTGGG CGGCATTTAC GAATATCTCG CCTAACGTTA AAGAAGAGGG AGCAATGAAA 
GAAGACGCTG GCACTCAGGA TACTCCATAC ACTGAGGACA CGCTGGTGGA GCAGCTGGAG 
ATGTGTGTTG ATTATCTCTG GAAGTCTGAG AGATATGAAC TTATTGCCGA GATCAACAAA 
CCTGTTATTG TCGTCTTTGA GAAGAGAAGA GATTTCAAGC GTCTATCAGA GCTGTACTAT 
GACATTCACC GCTCATACCT GAAGGTGACC GAAGTCGTGA ATTCTGAGAA ACGTCTGTTT 
GGTCGGTACT ATCGCGTGGC TTTCTACGGA CAGGCAGTGG GGTTTTTTGA GGAAGAGGAG 
AGTAAGGAGT TTATCTACAA GGAGCCCAAA CTCACAGGAC TGTCCGAAAT TTCTCAAAGA 
CTCCTCAAAC TCTACTCGGA CAAGTTTGGA GCTGATAACG TCAAAATGAT CCAGGACTCC 
AACAAGGTGA ATCCGAAAGA CTTGGACCCC AGATTTGCCT ACATCCAGGT CACCTATGTA 
GTGCCTTATT TTAATGAGAA GGAACAGCTG GAGAAGAAAA CCGACTTCGA GCGCCATCAC 
AACATCGACC GATTTGTGTT TGAGACGCCC TTCACGCTTT CTGGAAAAAA ACACGGAGAC 
GTGGAGGAAC AGTGCAAAAG GAAAACCATC CTGACCACGA GCTCTAGTTT CCCTTTCTTG 
AAGAAGCGTA TAGAGGTGGT GGAGCAACAG AGTACCGAGA TGAACCCTAT TGAGGTGGCG 
ATAGACGAGA TGAGCCGAAA AGTGTCTGAA CTCAAACAGC TGTGCAATAT GGAGGAGGTG 
GACATGATCC GTCTGCAGCT CAAACTACAG GGCAGCGTCA GCGTCAAGGT AAATGCAGGA 
CCCATGGCCT ACGCCCGAGC CTTCCTAGAG GAGAAAAATG CTAAGAAATA CCCAGACAAC 
CAGGTCAAAC TGCTGAAAGA GATCTTCAGA CAGTTTGCAG AAGCGTGTGG ACAGGCTCTG 
AGTGTGAACG AGCGGCTAAT AAAAGAGGAT CAGTTTGAGT ATCAGAGCGA GATGAAAGCT 
CACTATAAAG ACATGCTCAC GGAGCTCTCC GCCATTATGA ATGAGCAGAT ACCGTACACG 
AGACAACCAG GAACAGAGCG ACACAGTGCC ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOCK10 ( XM_021466298.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021321973.1 Danio rerio dedicator of cytokinesis 10 (dock10), transcript variant X3, mRNA.

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CloneID	ODa149006
Clone ID Related Accession (Same CDS sequence)	XM_021466298.1
Accession Version	XM_021466298.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	dedicator of cytokinesis protein 10 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAAAA TCACCTTTCG GAAGAAGATA AAGAGCAGAC GGGTTCGGGC AGACTGCAAC 
CGAGTGAGCT GTCAGGAGAA GCCCAAACTG ATCGACCCTC TGGACTATGA AGCTGTGATT 
TCTGAACTTG AAGCAGAATT CAGAGAAGAT CCTCTTCAAG ATCTCCTGTT ATTCCCAGAC 
CTTGACTTCA CTGTGTCCAC ACTTCCTGTA GAAAGGAGAA CAGTGAAGTC TACTGTCCCA 
GAGGGAGCTG ATGAACATGC TGAATGTCTG CTGGTCAGAC AGGCCTGCCA ATACTTCAAC 
TCCCAGCTGC ACATTGTTCA GTATAAATAT GATGAGTATG GAGGGGACTA CCGCATGCTG 
CCCAGGAAGC TGTGTAAAGC AGAAAAGTTG CCTTCCCACT CTTTTGAGAT TGACAATGAA 
GATGCGGACA AAGATGAGGA CACAACATCT TTGTCCTCAT CTAAAGGAGG GGGCGGCGGT 
GCAGGCAGTG GAGGCGGAGG AGGAATTGGT GTTTTTAAGT CCGGCTGGCT CTATAAAGGC 
AACTTCAACA GCACCGTCAA CAACAGCATC ACAGTTCGGT CCTTCAAGCG CCGATATTTT 
CAGCTGACTC AGCTCTCTGA CAACTCTTAC ATCATGAACT TCTATAAAGA TGAGAAAATT 
TCCAAGGAAC CGAAAGGCTG TATTTTTCTG GACTCCTGCA CCGGGGTGGT TCAGAATAAT 
CGACTGAGGA AACATGCGTT TGAGCTGAAA ATGAACGAAG TGACATATTT TGTCCTGGCT 
GCCGAGAATG AGCAAGACAT GGACGACTGG ATCAACACTT TAAATCGCAT CCTGCAGATC 
AGCCCTCCAG ACGGCCCGGC TCAGGACCGC AAGAGTACGG ACATGAGCGA CTCTAAAAAC 
GAGATCAGTG ATATACCACA AAATAACGTC CCTGTTCATA AAAACGAAGA AACAACTGAA 
AATGAGATCC ACCCTGAACT AGCCAAGTAT ATTTCTGAGA CAGAAGAAAG CTCGAGAGCT 
GTCAGAGCAG AGGAGAGACT GAACCTGTTC TCCCTGGACC CTGATATTCC TGTTTTAAGG 
AGTCCTAGGA CAGAAACCGG GGAGTCTTCT TCTGTCCATC CATTTGATGA GAAGCTTGGG 
AAGAGGCTGA TGATCTGCTG TCGCTCACTG AACCTCACAC TGCAAGGCTG CGTTAATGAA 
GCGGACACCG AACCCACCAC TAATATCGAG CCTTTCTTTG TGTCAGTGTG TCTGCTGGAC 
GTACGTGAGG GCAGGAAGGT TTCCGCTGAC TTCCATGTGG ACGTAAACCA TGAGTTAGTG 
CGGCAGATGC TTAATAGCTC TGGTAATCCG GGAGATCAAG GCTCGGATGT TGGTAATGGT 
GCACCGCTGG AAAATGGACT CACTTCCCCG GTAGAAAAGA AACCAGAAGA CTGTCAACTA 
GGCCCAGACA TGGAAAAGTG GCTATGCTTT CCCAAACAGG CAATCTTTTC CATTACGAAC 
CCGCACACGG ATATTGTGAT GGTGGCACGA GTGGAGAAAG TTCTGATGGG AAATATTTCC 
AGTGGTGCAG AGCCCTACAT CAAAAACACC GATTCCAGCA AGACTGTACA CAAGATGCTG 
AAATCCAATA AGCAGTTCTG TAGCAAACTA GGCAAGTACC GAATGCCTTT CGCCTGGTCT 
GTAAGGTCAG TGTTTAAGGA CAACCAGGGC ACAGTGGACA GGGAGTCTCG CTTCTCTCCT 
CTTTTCAAAC AGGAAAGCAA CAAGATTTCC ACCGAAGACC TCATCAAGCT CATCACCGAG 
TACAGGAGGG CGGAGAAGGC CAGTAAACTC CAGACCATCC CTGGAAACCT TGAAATAAAT 
GTGGACTGTG TTCCCATGGA ATGTCCCAAT TGTGTGACGT CCTCATATGT TGCCCTGAAG 
CCGTTTGAGG ACTGCAGCCA ACATGCTCCT ACGGTGGAAA TCGATGAGTT CCTGCAGGAT 
TCGTCAAAGT TCGCTCAACC TTACAGAGTC TACAAGAACC ACATCTACAT CTACCCTAAA 
CACCTCAAGT ATGACAGCCA GAAGAGCTTT GCTAAGGCTC GAAATATTGC AGTTTGTGTG 
GAGTTTCGAA GCTCCGATGA AGAACATGCC AAGCCGCTTA AGTGCATCTA TGGAAAGCCC 
GGAGGCCCAG TTTTTACCAC GACTGCCTGT ACCACAGTTC TGCACCATTC ACAGAACCCA 
GACTTTTATG ATGAGGTCAA GATTGAGTTG CCCACACATC TTCATGAGAA ACACCATATA 
CTCTTCTCCT TCTATCATGT GACCTGTGAC ATCAATGCCA AGACCAGCTC TAAGAAGAAA 
GAGGCTCTTG AATTGCCAGT GGGTTATGCA TGGCTGCCTC TTCTAAAGGA TGGACGCATT 
TCCTCTCAGG ACTTCAGCAT TCCTGTCTCC TGCACTTTGC CTGCTGGCTA TTTACACATT 
AAAGAGCCTT CGTCCACCAA GAATGGCTCT GATGTGAAGT GGGTCGATGG AGGGAAGCAG 
ATCTTCAAAT TGTCCACTTT GGTGCTTTCT ACAGTGTATA CTCAGGATCC CCATCTAAAC 
CGCTTCTTCC AGCAGTGCCA AAAACGAGAG GCAGAAATCT CTCAACCTCC TACCTCAAAC 
TTCCTCAACT GCCTTAAAGG GCTCTTGAGC ATGGAGAAGA TTCATGCCAT AATTCGCTTC 
CTTCCTGTTA TCTTCAACCA GCTCTTTAAA GTGCTTACGC AAAACGACTC TGATGAAGTC 
ACCACCAGCA CCACCAGGGT CCTGATACAC ATCTTAGCCA AGTGCCACGA GGAGAACCTG 
GACCACTATT TGCACTCCTA CATTAAGTTT GTCTTTAAGA CTGAAGCGTG TGGCTTCAGA 
ACCATTCATG AAGAGCTGGC CAAGGGAATG ACCTCTGACC TGAAGAGCAA TGAACAGTTA 
GTCATACGCA ACGTCCTGAA GTTCTCGTGG TTCTTTCTCG AGATCATAGT GAAGTCAATG 
GCTCAGCATC TGCTGGACTC AAACAAGCTC AAGCTGCCGC GACCGCAACG ATTTCCTGGC 
ACTTTCCAAA ACCGTTTGGA GACGTTGATC ATGACCATGT CGGATCACAT TTACTGGAAG 
AACAAAGAGC TGGCGGAGGA GACGCGGAGT GCAAATATGG CCATTGCGTT TTTTGTGAAG 
CGGTGCTTCA CCTTGATGGA CAGAGGTTTC ACCTTTAAAC TGATCAGCAA CTACCTGAAC 
ATGATCACAG CCACTGACAA TAAGGCGCTG TGTGAGCTGA AGTTTGAGTT CATAAGGGAA 
GTGTGTAACT ATGAGCACTT TGTCCCTTTG AGTCTTCCCA TCCCGTCTGC ACGAATCACA 
GACAATGCAT CAACAGAAGC CCAAAGTACT AAGGTGGACT TCCCAGAGTA CAGTTTGACA 
GAGGATTTCT GCAGGAAGCA TTTTCTGTCT GGATTGTTGT TGCGAGAGCT GGGACTCGCT 
TTGCAGGATG AGCAAGATCT GAGACACATC GCTCTGGCCT GCCTGAAGAA CCTGATGGCC 
AAACACTCGC TGGACGCTCG ATACGCCACA AAGGAGAAAC AGGCTCGGAT AGCTTCTCTT 
TACCTGCCGC TTTATGGCCT GATCCTGGAC AACATGCCTC GGTTTTTCCT CAGAGATCTG 
TTTCCAATCT GCCTGACAGC GAGTGACCAG GGATCCAGAG ATGATCTCAG TGTTGGAGTA 
GGTTTGGGTG GTCAGGTGAC CCATGTTTTA CGCCATGGAA ACTCAATGGA CGCGTCCTTC 
TCCAAGGAAG TGCTCAACTC CATTACAGCC TTTTCATCAT TGGCTGTATC GACCGCTAAT 
CATGCCGACT CCAGGGGTTC TCTGATCAGC ATCGAGTCGA ACCCCAGCAA CAGTGACCGC 
AACAGTGAGA AGATGGATGC CTGTGAAAAG TTTGCCAGGC CTCAGTCTCT TATTGGCTTT 
GGATCCCGCT TTGATAAACT GGATCAAGCT GAAACCAGAA GTCTGCTCAT GTGTTTCCTG 
CACATTATGA AGACCATCTC AGAGGAGGTG ATGGTTTCAT ACTGGCATCG AGCGATTCAC 
CAGGAGATCA GTGACTTCTT CAATATTTTG GAATTATGCC TTCAGCACTT TCGTTTCCTT 
GGAAAGCGGC ACATTGCCAG GAAGCTAGCG GCCGCAGTTA AGCTGGCTCA GGCCACACAG 
AACAATGGCA CCCTGAAGGG CTCTAATGTG TCCTACCACT CTCCAGGGCT CCTACCCCAA 
TGGATTCTGT CTGCACAGGA CGGTCACAGA CACGCTCGCT CTCAGACCAT GCCCATCATC 
AGGGGGAAAA ACGCCCTCAC AAACCCCAAA CTGCTGCATA TGATGGAGAC AGATGGAAAT 
GTTGCTGAAG GAGAAAACTT TAACCCTACA GACGTGGAGG CGAACCTTTC TACTGAAGTA 
GCACTGACCG TGTTGGATGT TCTGGACCTG TTTGTTCGAC ATCATAAGAA ACAGTTGCAG 
CACGATGAAG GACAAAACTC TTTGATGAAG AAAGTGTTCG ACACGTACCT GCTGTTCTTC 
CAGATCAACC AGTCCACCAC CACCCTCAGA CACGTGTTCA CAGCCCTGCG CCTCTTCATG 
CAGAAGTTTC CGTCCACGTT TTTCCAGGGT AAAGCGGATG TGTGTGGCAG CCTGTGTTAC 
GAGATCCTGA AGTGCTGTAA CCATCGCTCT AGCTCCACAC AGACTGAAGC CGCCGCTCTG 
CTTTATTTCC TCATGAGGAG GAACTTTGAG TTCACGAAGG GCAAGTGCAT CGTCCGCTCA 
CACCTGCAGG TGATTAAAGC TGTGAGTCAG CTGATCGCGG ATGCAGGCAT TGGCGGATCA 
CGCTTCCAGC AGTCTCTGGC CATCATCAAT AACTTCGCCA ATGGGGACAC GCCACTTAAA 
AACACCACAT TCCCAGCTGA CGTAAAGGAC CTGACCAAGA GGATACGCAC CGTCCTGATG 
GCCACGTCTC AAATGAAAGA GCATGAAAAA GACCCGGAAA TGCTGGTGGA CCTTCAGTAC 
AGTCTGGCCA ACTCCTACGC CAGCACACCT GAGCTCAGAC GCACCTGGCT GGAAAGCATG 
GCCAAAATAC ACGTCAGAAA TGGAGATCTG TCTGAGGCAG CCATGTGCTA CATCCACATC 
TCAGCCCTCA TAGCAGAGTC TCTGAGGAGA AGAGGTTACT GGAAGTCTGA AAAGGGTCGG 
ATGTCAAGTG TGAGCAAAGA GGGAGCCTGT GTAAATAACT GTAGCCCTTT ACTAACTCCC 
CAAGACGGAG AAACATCATT CAGTATGGGT TGGGCGGCAT TTACGAATAT CTCGCCTAAC 
GTTAAAGAAG AGGGAGCAAT GAAAGAAGAC GCTGGCACTC AGGATACTCC ATACACTGAG 
GACACGCTGG TGGAGCAGCT GGAGATGTGT GTTGATTATC TCTGGAAGTC TGAGAGATAT 
GAACTTATTG CCGAGATCAA CAAACCTGTT ATTGTCGTCT TTGAGAAGAG AAGAGATTTC 
AAGCGTCTAT CAGAGCTGTA CTATGACATT CACCGCTCAT ACCTGAAGGT GACCGAAGTC 
GTGAATTCTG AGAAACGTCT GTTTGGTCGG TACTATCGCG TGGCTTTCTA CGGACAGGCA 
GTGGGGTTTT TTGAGGAAGA GGAGAGTAAG GAGTTTATCT ACAAGGAGCC CAAACTCACA 
GGACTGTCCG AAATTTCTCA AAGACTCCTC AAACTCTACT CGGACAAGTT TGGAGCTGAT 
AACGTCAAAA TGATCCAGGA CTCCAACAAG GTGAATCCGA AAGACTTGGA CCCCAGATTT 
GCCTACATCC AGGTCACCTA TGTAGTGCCT TATTTTAATG AGAAGGAACA GCTGGAGAAG 
AAAACCGACT TCGAGCGCCA TCACAACATC GACCGATTTG TGTTTGAGAC GCCCTTCACG 
CTTTCTGGAA AAAAACACGG AGACGTGGAG GAACAGTGCA AAAGGAAAAC CATCCTGACC 
ACGAGCTCTA GTTTCCCTTT CTTGAAGAAG CGTATAGAGG TGGTGGAGCA ACAGAGTACC 
GAGATGAACC CTATTGAGGT GGCGATAGAC GAGATGAGCC GAAAAGTGTC TGAACTCAAA 
CAGCTGTGCA ATATGGAGGA GGTGGACATG ATCCGTCTGC AGCTCAAACT ACAGGGCAGC 
GTCAGCGTCA AGGTAAATGC AGGACCCATG GCCTACGCCC GAGCCTTCCT AGAGGAGAAA 
AATGCTAAGA AATACCCAGA CAACCAGGTC AAACTGCTGA AAGAGATCTT CAGACAGTTT 
GCAGAAGCGT GTGGACAGGC TCTGAGTGTG AACGAGCGGC TAATAAAAGA GGATCAGTTT 
GAGTATCAGA GCGAGATGAA AGCTCACTAT AAAGACATGC TCACGGAGCT CTCCGCCATT 
ATGAATGAGC AGATACCGTA CACGAGACAA CCAGGAACAG AGCGACACAG TGCCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021321973.1
CDS	93..6572
Translation	MSKITFRKKIKSRRVRADCNRVSCQEKPKLIDPLDYEAVISELEAEFREDPLQDLLLFPDLDFTVSTLPVERRTVKSTVPEGADEHAECLLVRQACQYFNSQLHIVQYKYDEYGGDYRMLPRKLCKAEKLPSHSFEIDNEDADKDEDTTSLSSSKGGGGGAGSGGGGGIGVFKSGWLYKGNFNSTVNNSITVRSFKRRYFQLTQLSDNSYIMNFYKDEKISKEPKGCIFLDSCTGVVQNNRLRKHAFELKMNEVTYFVLAAENEQDMDDWINTLNRILQISPPDGPAQDRKSTDMSDSKNEISDIPQNNVPVHKNEETTENEIHPELAKYISETEESSRAVRAEERLNLFSLDPDIPVLRSPRTETGESSSVHPFDEKLGKRLMICCRSLNLTLQGCVNEADTEPTTNIEPFFVSVCLLDVREGRKVSADFHVDVNHELVRQMLNSSGNPGDQGSDVGNGAPLENGLTSPVEKKPEDCQLGPDMEKWLCFPKQAIFSITNPHTDIVMVARVEKVLMGNISSGAEPYIKNTDSSKTVHKMLKSNKQFCSKLGKYRMPFAWSVRSVFKDNQGTVDRESRFSPLFKQESNKISTEDLIKLITEYRRAEKASKLQTIPGNLEINVDCVPMECPNCVTSSYVALKPFEDCSQHAPTVEIDEFLQDSSKFAQPYRVYKNHIYIYPKHLKYDSQKSFAKARNIAVCVEFRSSDEEHAKPLKCIYGKPGGPVFTTTACTTVLHHSQNPDFYDEVKIELPTHLHEKHHILFSFYHVTCDINAKTSSKKKEALELPVGYAWLPLLKDGRISSQDFSIPVSCTLPAGYLHIKEPSSTKNGSDVKWVDGGKQIFKLSTLVLSTVYTQDPHLNRFFQQCQKREAEISQPPTSNFLNCLKGLLSMEKIHAIIRFLPVIFNQLFKVLTQNDSDEVTTSTTRVLIHILAKCHEENLDHYLHSYIKFVFKTEACGFRTIHEELAKGMTSDLKSNEQLVIRNVLKFSWFFLEIIVKSMAQHLLDSNKLKLPRPQRFPGTFQNRLETLIMTMSDHIYWKNKELAEETRSANMAIAFFVKRCFTLMDRGFTFKLISNYLNMITATDNKALCELKFEFIREVCNYEHFVPLSLPIPSARITDNASTEAQSTKVDFPEYSLTEDFCRKHFLSGLLLRELGLALQDEQDLRHIALACLKNLMAKHSLDARYATKEKQARIASLYLPLYGLILDNMPRFFLRDLFPICLTASDQGSRDDLSVGVGLGGQVTHVLRHGNSMDASFSKEVLNSITAFSSLAVSTANHADSRGSLISIESNPSNSDRNSEKMDACEKFARPQSLIGFGSRFDKLDQAETRSLLMCFLHIMKTISEEVMVSYWHRAIHQEISDFFNILELCLQHFRFLGKRHIARKLAAAVKLAQATQNNGTLKGSNVSYHSPGLLPQWILSAQDGHRHARSQTMPIIRGKNALTNPKLLHMMETDGNVAEGENFNPTDVEANLSTEVALTVLDVLDLFVRHHKKQLQHDEGQNSLMKKVFDTYLLFFQINQSTTTLRHVFTALRLFMQKFPSTFFQGKADVCGSLCYEILKCCNHRSSSTQTEAAALLYFLMRRNFEFTKGKCIVRSHLQVIKAVSQLIADAGIGGSRFQQSLAIINNFANGDTPLKNTTFPADVKDLTKRIRTVLMATSQMKEHEKDPEMLVDLQYSLANSYASTPELRRTWLESMAKIHVRNGDLSEAAMCYIHISALIAESLRRRGYWKSEKGRMSSVSKEGACVNNCSPLLTPQDGETSFSMGWAAFTNISPNVKEEGAMKEDAGTQDTPYTEDTLVEQLEMCVDYLWKSERYELIAEINKPVIVVFEKRRDFKRLSELYYDIHRSYLKVTEVVNSEKRLFGRYYRVAFYGQAVGFFEEEESKEFIYKEPKLTGLSEISQRLLKLYSDKFGADNVKMIQDSNKVNPKDLDPRFAYIQVTYVVPYFNEKEQLEKKTDFERHHNIDRFVFETPFTLSGKKHGDVEEQCKRKTILTTSSSFPFLKKRIEVVEQQSTEMNPIEVAIDEMSRKVSELKQLCNMEEVDMIRLQLKLQGSVSVKVNAGPMAYARAFLEEKNAKKYPDNQVKLLKEIFRQFAEACGQALSVNERLIKEDQFEYQSEMKAHYKDMLTELSAIMNEQIPYTRQPGTERHSAI

Target ORF information:

RefSeq Version	XM_021466298.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio dedicator of cytokinesis 10 (dock10), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021466298.1

ORF Insert Sequence:

ATGAGTAAAA TCACCTTTCG GAAGAAGATA AAGAGCAGAC GGGTTCGGGC AGACTGCAAC 
CGAGTGAGCT GTCAGGAGAA GCCCAAACTG ATCGACCCTC TGGACTATGA AGCTGTGATT 
TCTGAACTTG AAGCAGAATT CAGAGAAGAT CCTCTTCAAG ATCTCCTGTT ATTCCCAGAC 
CTTGACTTCA CTGTGTCCAC ACTTCCTGTA GAAAGGAGAA CAGTGAAGTC TACTGTCCCA 
GAGGGAGCTG ATGAACATGC TGAATGTCTG CTGGTCAGAC AGGCCTGCCA ATACTTCAAC 
TCCCAGCTGC ACATTGTTCA GTATAAATAT GATGAGTATG GAGGGGACTA CCGCATGCTG 
CCCAGGAAGC TGTGTAAAGC AGAAAAGTTG CCTTCCCACT CTTTTGAGAT TGACAATGAA 
GATGCGGACA AAGATGAGGA CACAACATCT TTGTCCTCAT CTAAAGGAGG GGGCGGCGGT 
GCAGGCAGTG GAGGCGGAGG AGGAATTGGT GTTTTTAAGT CCGGCTGGCT CTATAAAGGC 
AACTTCAACA GCACCGTCAA CAACAGCATC ACAGTTCGGT CCTTCAAGCG CCGATATTTT 
CAGCTGACTC AGCTCTCTGA CAACTCTTAC ATCATGAACT TCTATAAAGA TGAGAAAATT 
TCCAAGGAAC CGAAAGGCTG TATTTTTCTG GACTCCTGCA CCGGGGTGGT TCAGAATAAT 
CGACTGAGGA AACATGCGTT TGAGCTGAAA ATGAACGAAG TGACATATTT TGTCCTGGCT 
GCCGAGAATG AGCAAGACAT GGACGACTGG ATCAACACTT TAAATCGCAT CCTGCAGATC 
AGCCCTCCAG ACGGCCCGGC TCAGGACCGC AAGAGTACGG ACATGAGCGA CTCTAAAAAC 
GAGATCAGTG ATATACCACA AAATAACGTC CCTGTTCATA AAAACGAAGA AACAACTGAA 
AATGAGATCC ACCCTGAACT AGCCAAGTAT ATTTCTGAGA CAGAAGAAAG CTCGAGAGCT 
GTCAGAGCAG AGGAGAGACT GAACCTGTTC TCCCTGGACC CTGATATTCC TGTTTTAAGG 
AGTCCTAGGA CAGAAACCGG GGAGTCTTCT TCTGTCCATC CATTTGATGA GAAGCTTGGG 
AAGAGGCTGA TGATCTGCTG TCGCTCACTG AACCTCACAC TGCAAGGCTG CGTTAATGAA 
GCGGACACCG AACCCACCAC TAATATCGAG CCTTTCTTTG TGTCAGTGTG TCTGCTGGAC 
GTACGTGAGG GCAGGAAGGT TTCCGCTGAC TTCCATGTGG ACGTAAACCA TGAGTTAGTG 
CGGCAGATGC TTAATAGCTC TGGTAATCCG GGAGATCAAG GCTCGGATGT TGGTAATGGT 
GCACCGCTGG AAAATGGACT CACTTCCCCG GTAGAAAAGA AACCAGAAGA CTGTCAACTA 
GGCCCAGACA TGGAAAAGTG GCTATGCTTT CCCAAACAGG CAATCTTTTC CATTACGAAC 
CCGCACACGG ATATTGTGAT GGTGGCACGA GTGGAGAAAG TTCTGATGGG AAATATTTCC 
AGTGGTGCAG AGCCCTACAT CAAAAACACC GATTCCAGCA AGACTGTACA CAAGATGCTG 
AAATCCAATA AGCAGTTCTG TAGCAAACTA GGCAAGTACC GAATGCCTTT CGCCTGGTCT 
GTAAGGTCAG TGTTTAAGGA CAACCAGGGC ACAGTGGACA GGGAGTCTCG CTTCTCTCCT 
CTTTTCAAAC AGGAAAGCAA CAAGATTTCC ACCGAAGACC TCATCAAGCT CATCACCGAG 
TACAGGAGGG CGGAGAAGGC CAGTAAACTC CAGACCATCC CTGGAAACCT TGAAATAAAT 
GTGGACTGTG TTCCCATGGA ATGTCCCAAT TGTGTGACGT CCTCATATGT TGCCCTGAAG 
CCGTTTGAGG ACTGCAGCCA ACATGCTCCT ACGGTGGAAA TCGATGAGTT CCTGCAGGAT 
TCGTCAAAGT TCGCTCAACC TTACAGAGTC TACAAGAACC ACATCTACAT CTACCCTAAA 
CACCTCAAGT ATGACAGCCA GAAGAGCTTT GCTAAGGCTC GAAATATTGC AGTTTGTGTG 
GAGTTTCGAA GCTCCGATGA AGAACATGCC AAGCCGCTTA AGTGCATCTA TGGAAAGCCC 
GGAGGCCCAG TTTTTACCAC GACTGCCTGT ACCACAGTTC TGCACCATTC ACAGAACCCA 
GACTTTTATG ATGAGGTCAA GATTGAGTTG CCCACACATC TTCATGAGAA ACACCATATA 
CTCTTCTCCT TCTATCATGT GACCTGTGAC ATCAATGCCA AGACCAGCTC TAAGAAGAAA 
GAGGCTCTTG AATTGCCAGT GGGTTATGCA TGGCTGCCTC TTCTAAAGGA TGGACGCATT 
TCCTCTCAGG ACTTCAGCAT TCCTGTCTCC TGCACTTTGC CTGCTGGCTA TTTACACATT 
AAAGAGCCTT CGTCCACCAA GAATGGCTCT GATGTGAAGT GGGTCGATGG AGGGAAGCAG 
ATCTTCAAAT TGTCCACTTT GGTGCTTTCT ACAGTGTATA CTCAGGATCC CCATCTAAAC 
CGCTTCTTCC AGCAGTGCCA AAAACGAGAG GCAGAAATCT CTCAACCTCC TACCTCAAAC 
TTCCTCAACT GCCTTAAAGG GCTCTTGAGC ATGGAGAAGA TTCATGCCAT AATTCGCTTC 
CTTCCTGTTA TCTTCAACCA GCTCTTTAAA GTGCTTACGC AAAACGACTC TGATGAAGTC 
ACCACCAGCA CCACCAGGGT CCTGATACAC ATCTTAGCCA AGTGCCACGA GGAGAACCTG 
GACCACTATT TGCACTCCTA CATTAAGTTT GTCTTTAAGA CTGAAGCGTG TGGCTTCAGA 
ACCATTCATG AAGAGCTGGC CAAGGGAATG ACCTCTGACC TGAAGAGCAA TGAACAGTTA 
GTCATACGCA ACGTCCTGAA GTTCTCGTGG TTCTTTCTCG AGATCATAGT GAAGTCAATG 
GCTCAGCATC TGCTGGACTC AAACAAGCTC AAGCTGCCGC GACCGCAACG ATTTCCTGGC 
ACTTTCCAAA ACCGTTTGGA GACGTTGATC ATGACCATGT CGGATCACAT TTACTGGAAG 
AACAAAGAGC TGGCGGAGGA GACGCGGAGT GCAAATATGG CCATTGCGTT TTTTGTGAAG 
CGGTGCTTCA CCTTGATGGA CAGAGGTTTC ACCTTTAAAC TGATCAGCAA CTACCTGAAC 
ATGATCACAG CCACTGACAA TAAGGCGCTG TGTGAGCTGA AGTTTGAGTT CATAAGGGAA 
GTGTGTAACT ATGAGCACTT TGTCCCTTTG AGTCTTCCCA TCCCGTCTGC ACGAATCACA 
GACAATGCAT CAACAGAAGC CCAAAGTACT AAGGTGGACT TCCCAGAGTA CAGTTTGACA 
GAGGATTTCT GCAGGAAGCA TTTTCTGTCT GGATTGTTGT TGCGAGAGCT GGGACTCGCT 
TTGCAGGATG AGCAAGATCT GAGACACATC GCTCTGGCCT GCCTGAAGAA CCTGATGGCC 
AAACACTCGC TGGACGCTCG ATACGCCACA AAGGAGAAAC AGGCTCGGAT AGCTTCTCTT 
TACCTGCCGC TTTATGGCCT GATCCTGGAC AACATGCCTC GGTTTTTCCT CAGAGATCTG 
TTTCCAATCT GCCTGACAGC GAGTGACCAG GGATCCAGAG ATGATCTCAG TGTTGGAGTA 
GGTTTGGGTG GTCAGGTGAC CCATGTTTTA CGCCATGGAA ACTCAATGGA CGCGTCCTTC 
TCCAAGGAAG TGCTCAACTC CATTACAGCC TTTTCATCAT TGGCTGTATC GACCGCTAAT 
CATGCCGACT CCAGGGGTTC TCTGATCAGC ATCGAGTCGA ACCCCAGCAA CAGTGACCGC 
AACAGTGAGA AGATGGATGC CTGTGAAAAG TTTGCCAGGC CTCAGTCTCT TATTGGCTTT 
GGATCCCGCT TTGATAAACT GGATCAAGCT GAAACCAGAA GTCTGCTCAT GTGTTTCCTG 
CACATTATGA AGACCATCTC AGAGGAGGTG ATGGTTTCAT ACTGGCATCG AGCGATTCAC 
CAGGAGATCA GTGACTTCTT CAATATTTTG GAATTATGCC TTCAGCACTT TCGTTTCCTT 
GGAAAGCGGC ACATTGCCAG GAAGCTAGCG GCCGCAGTTA AGCTGGCTCA GGCCACACAG 
AACAATGGCA CCCTGAAGGG CTCTAATGTG TCCTACCACT CTCCAGGGCT CCTACCCCAA 
TGGATTCTGT CTGCACAGGA CGGTCACAGA CACGCTCGCT CTCAGACCAT GCCCATCATC 
AGGGGGAAAA ACGCCCTCAC AAACCCCAAA CTGCTGCATA TGATGGAGAC AGATGGAAAT 
GTTGCTGAAG GAGAAAACTT TAACCCTACA GACGTGGAGG CGAACCTTTC TACTGAAGTA 
GCACTGACCG TGTTGGATGT TCTGGACCTG TTTGTTCGAC ATCATAAGAA ACAGTTGCAG 
CACGATGAAG GACAAAACTC TTTGATGAAG AAAGTGTTCG ACACGTACCT GCTGTTCTTC 
CAGATCAACC AGTCCACCAC CACCCTCAGA CACGTGTTCA CAGCCCTGCG CCTCTTCATG 
CAGAAGTTTC CGTCCACGTT TTTCCAGGGT AAAGCGGATG TGTGTGGCAG CCTGTGTTAC 
GAGATCCTGA AGTGCTGTAA CCATCGCTCT AGCTCCACAC AGACTGAAGC CGCCGCTCTG 
CTTTATTTCC TCATGAGGAG GAACTTTGAG TTCACGAAGG GCAAGTGCAT CGTCCGCTCA 
CACCTGCAGG TGATTAAAGC TGTGAGTCAG CTGATCGCGG ATGCAGGCAT TGGCGGATCA 
CGCTTCCAGC AGTCTCTGGC CATCATCAAT AACTTCGCCA ATGGGGACAC GCCACTTAAA 
AACACCACAT TCCCAGCTGA CGTAAAGGAC CTGACCAAGA GGATACGCAC CGTCCTGATG 
GCCACGTCTC AAATGAAAGA GCATGAAAAA GACCCGGAAA TGCTGGTGGA CCTTCAGTAC 
AGTCTGGCCA ACTCCTACGC CAGCACACCT GAGCTCAGAC GCACCTGGCT GGAAAGCATG 
GCCAAAATAC ACGTCAGAAA TGGAGATCTG TCTGAGGCAG CCATGTGCTA CATCCACATC 
TCAGCCCTCA TAGCAGAGTC TCTGAGGAGA AGAGGTTACT GGAAGTCTGA AAAGGGTCGG 
ATGTCAAGTG TGAGCAAAGA GGGAGCCTGT GTAAATAACT GTAGCCCTTT ACTAACTCCC 
CAAGACGGAG AAACATCATT CAGTATGGGT TGGGCGGCAT TTACGAATAT CTCGCCTAAC 
GTTAAAGAAG AGGGAGCAAT GAAAGAAGAC GCTGGCACTC AGGATACTCC ATACACTGAG 
GACACGCTGG TGGAGCAGCT GGAGATGTGT GTTGATTATC TCTGGAAGTC TGAGAGATAT 
GAACTTATTG CCGAGATCAA CAAACCTGTT ATTGTCGTCT TTGAGAAGAG AAGAGATTTC 
AAGCGTCTAT CAGAGCTGTA CTATGACATT CACCGCTCAT ACCTGAAGGT GACCGAAGTC 
GTGAATTCTG AGAAACGTCT GTTTGGTCGG TACTATCGCG TGGCTTTCTA CGGACAGGCA 
GTGGGGTTTT TTGAGGAAGA GGAGAGTAAG GAGTTTATCT ACAAGGAGCC CAAACTCACA 
GGACTGTCCG AAATTTCTCA AAGACTCCTC AAACTCTACT CGGACAAGTT TGGAGCTGAT 
AACGTCAAAA TGATCCAGGA CTCCAACAAG GTGAATCCGA AAGACTTGGA CCCCAGATTT 
GCCTACATCC AGGTCACCTA TGTAGTGCCT TATTTTAATG AGAAGGAACA GCTGGAGAAG 
AAAACCGACT TCGAGCGCCA TCACAACATC GACCGATTTG TGTTTGAGAC GCCCTTCACG 
CTTTCTGGAA AAAAACACGG AGACGTGGAG GAACAGTGCA AAAGGAAAAC CATCCTGACC 
ACGAGCTCTA GTTTCCCTTT CTTGAAGAAG CGTATAGAGG TGGTGGAGCA ACAGAGTACC 
GAGATGAACC CTATTGAGGT GGCGATAGAC GAGATGAGCC GAAAAGTGTC TGAACTCAAA 
CAGCTGTGCA ATATGGAGGA GGTGGACATG ATCCGTCTGC AGCTCAAACT ACAGGGCAGC 
GTCAGCGTCA AGGTAAATGC AGGACCCATG GCCTACGCCC GAGCCTTCCT AGAGGAGAAA 
AATGCTAAGA AATACCCAGA CAACCAGGTC AAACTGCTGA AAGAGATCTT CAGACAGTTT 
GCAGAAGCGT GTGGACAGGC TCTGAGTGTG AACGAGCGGC TAATAAAAGA GGATCAGTTT 
GAGTATCAGA GCGAGATGAA AGCTCACTAT AAAGACATGC TCACGGAGCT CTCCGCCATT 
ATGAATGAGC AGATACCGTA CACGAGACAA CCAGGAACAG AGCGACACAG TGCCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOCK10 ( XM_017351136.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017206625.1 Danio rerio dedicator of cytokinesis 10 (dock10), transcript variant X2, mRNA.

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CloneID	ODa55221
Clone ID Related Accession (Same CDS sequence)	XM_017351136.1
Accession Version	XM_017351136.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	dedicator of cytokinesis protein 10 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTGTA CCACGAGTCT ACTACAGGCT GTGAATTTTA TAATGAGGAG AAACCATTCA 
CGCGTCTTTT CTGACCAGCA GAAACAACAT TTGCTGGAAA CTGCACCGCT GGAGGAGGGG 
ATGGTCATCA GTGAACCACA CAGCACTGTC GTCATGCAGG AGAAGCCCAA ACTGATCGAC 
CCTCTGGACT ATGAAGCTGT GATTTCTGAA CTTGAAGCAG AATTCAGAGA AGATCCTCTT 
CAAGATCTCC TGTTATTCCC AGACCTTGAC TTCACTGTGT CCACACTTCC TGTAGAAAGG 
AGAACAGTGA AGTCTACTGT CCCAGAGGGA GCTGATGAAC ATGCTGAATG TCTGCTGGTC 
AGACAGGCCT GCCAATACTT CAACTCCCAG CTGCACATTG TTCAGTATAA ATATGATGAG 
TATGGAGGGG ACTACCGCAT GCTGCCCAGG AAGCTGTGTA AAGCAGAAAA GTTGCCTTCC 
CACTCTTTTG AGATTGACAA TGAAGATGCG GACAAAGATG AGGACACAAC ATCTTTGTCC 
TCATCTAAAG GAGGGGGCGG CGGTGCAGGC AGTGGAGGCG GAGGAGGAAT TGGTGTTTTT 
AAGTCCGGCT GGCTCTATAA AGGCAACTTC AACAGCACCG TCAACAACAG CATCACAGTT 
CGGTCCTTCA AGCGCCGATA TTTTCAGCTG ACTCAGCTCT CTGACAACTC TTACATCATG 
AACTTCTATA AAGATGAGAA AATTTCCAAG GAACCGAAAG GCTGTATTTT TCTGGACTCC 
TGCACCGGGG TGGTTCAGAA TAATCGACTG AGGAAACATG CGTTTGAGCT GAAAATGAAC 
GAAGTGACAT ATTTTGTCCT GGCTGCCGAG AATGAGCAAG ACATGGACGA CTGGATCAAC 
ACTTTAAATC GCATCCTGCA GATCAGCCCT CCAGACGGCC CGGCTCAGGA CCGCAAGAGT 
ACGGACATGA GCGACTCTAA AAACGAGATC AGTGATATAC CACAAAATAA CGTCCCTGTT 
CATAAAAACG AAGAAACAAC TGAAAATGAG ATCCACCCTG AACTAGCCAA GTATATTTCT 
GAGACAGAAG AAAGCTCGAG AGCTGTCAGA GCAGAGGAGA GACTGAACCT GTTCTCCCTG 
GACCCTGATA TTCCTGTTTT AAGGAGTCCT AGGACAGAAA CCGGGGAGTC TTCTTCTGTC 
CATCCATTTG ATGAGAAGCT TGGGAAGAGG CTGATGATCT GCTGTCGCTC ACTGAACCTC 
ACACTGCAAG GCTGCGTTAA TGAAGCGGAC ACCGAACCCA CCACTAATAT CGAGCCTTTC 
TTTGTGTCAG TGTGTCTGCT GGACGTACGT GAGGGCAGGA AGGTTTCCGC TGACTTCCAT 
GTGGACGTAA ACCATGAGTT AGTGCGGCAG ATGCTTAATA GCTCTGGTAA TCCGGGAGAT 
CAAGGCTCGG ATGTTGGTAA TGGTGCACCG CTGGAAAATG GACTCACTTC CCCGGTAGAA 
AAGAAACCAG AAGACTGTCA ACTAGGCCCA GACATGGAAA AGTGGCTATG CTTTCCCAAA 
CAGGCAATCT TTTCCATTAC GAACCCGCAC ACGGATATTG TGATGGTGGC ACGAGTGGAG 
AAAGTTCTGA TGGGAAATAT TTCCAGTGGT GCAGAGCCCT ACATCAAAAA CACCGATTCC 
AGCAAGACTG TACACAAGAT GCTGAAATCC AATAAGCAGT TCTGTAGCAA ACTAGGCAAG 
TACCGAATGC CTTTCGCCTG GTCTGTAAGG TCAGTGTTTA AGGACAACCA GGGCACAGTG 
GACAGGGAGT CTCGCTTCTC TCCTCTTTTC AAACAGGAAA GCAACAAGAT TTCCACCGAA 
GACCTCATCA AGCTCATCAC CGAGTACAGG AGGGCGGAGA AGGCCAGTAA ACTCCAGACC 
ATCCCTGGAA ACCTTGAAAT AAATGTGGAC TGTGTTCCCA TGGAATGTCC CAATTGTGTG 
ACGTCCTCAT ATGTTGCCCT GAAGCCGTTT GAGGACTGCA GCCAACATGC TCCTACGGTG 
GAAATCGATG AGTTCCTGCA GGATTCGTCA AAGTTCGCTC AACCTTACAG AGTCTACAAG 
AACCACATCT ACATCTACCC TAAACACCTC AAGTATGACA GCCAGAAGAG CTTTGCTAAG 
GCTCGAAATA TTGCAGTTTG TGTGGAGTTT CGAAGCTCCG ATGAAGAACA TGCCAAGCCG 
CTTAAGTGCA TCTATGGAAA GCCCGGAGGC CCAGTTTTTA CCACGACTGC CTGTACCACA 
GTTCTGCACC ATTCACAGAA CCCAGACTTT TATGATGAGG TCAAGATTGA GTTGCCCACA 
CATCTTCATG AGAAACACCA TATACTCTTC TCCTTCTATC ATGTGACCTG TGACATCAAT 
GCCAAGACCA GCTCTAAGAA GAAAGAGGCT CTTGAATTGC CAGTGGGTTA TGCATGGCTG 
CCTCTTCTAA AGGATGGACG CATTTCCTCT CAGGACTTCA GCATTCCTGT CTCCTGCACT 
TTGCCTGCTG GCTATTTACA CATTAAAGAG CCTTCGTCCA CCAAGAATGG CTCTGATGTG 
AAGTGGGTCG ATGGAGGGAA GCAGATCTTC AAATTGTCCA CTTTGGTGCT TTCTACAGTG 
TATACTCAGG ATCCCCATCT AAACCGCTTC TTCCAGCAGT GCCAAAAACG AGAGGCAGAA 
ATCTCTCAAC CTCCTACCTC AAACTTCCTC AACTGCCTTA AAGGGCTCTT GAGCATGGAG 
AAGATTCATG CCATAATTCG CTTCCTTCCT GTTATCTTCA ACCAGCTCTT TAAAGTGCTT 
ACGCAAAACG ACTCTGATGA AGTCACCACC AGCACCACCA GGGTCCTGAT ACACATCTTA 
GCCAAGTGCC ACGAGGAGAA CCTGGACCAC TATTTGCACT CCTACATTAA GTTTGTCTTT 
AAGACTGAAG CGTGTGGCTT CAGAACCATT CATGAAGAGC TGGCCAAGGG AATGACCTCT 
GACCTGAAGA GCAATGAACA GTTAGTCATA CGCAACGTCC TGAAGTTCTC GTGGTTCTTT 
CTCGAGATCA TAGTGAAGTC AATGGCTCAG CATCTGCTGG ACTCAAACAA GCTCAAGCTG 
CCGCGACCGC AACGATTTCC TGGCACTTTC CAAAACCGTT TGGAGACGTT GATCATGACC 
ATGTCGGATC ACATTTACTG GAAGAACAAA GAGCTGGCGG AGGAGACGCG GAGTGCAAAT 
ATGGCCATTG CGTTTTTTGT GAAGCGGTGC TTCACCTTGA TGGACAGAGG TTTCACCTTT 
AAACTGATCA GCAACTACCT GAACATGATC ACAGCCACTG ACAATAAGGC GCTGTGTGAG 
CTGAAGTTTG AGTTCATAAG GGAAGTGTGT AACTATGAGC ACTTTGTCCC TTTGAGTCTT 
CCCATCCCGT CTGCACGAAT CACAGACAAT GCATCAACAG AAGCCCAAAG TACTAAGGTG 
GACTTCCCAG AGTACAGTTT GACAGAGGAT TTCTGCAGGA AGCATTTTCT GTCTGGATTG 
TTGTTGCGAG AGCTGGGACT CGCTTTGCAG GATGAGCAAG ATCTGAGACA CATCGCTCTG 
GCCTGCCTGA AGAACCTGAT GGCCAAACAC TCGCTGGACG CTCGATACGC CACAAAGGAG 
AAACAGGCTC GGATAGCTTC TCTTTACCTG CCGCTTTATG GCCTGATCCT GGACAACATG 
CCTCGGTTTT TCCTCAGAGA TCTGTTTCCA ATCTGCCTGA CAGCGAGTGA CCAGGGATCC 
AGAGATGATC TCAGTGTTGG AGTAGGTTTG GGTGGTCAGG TGACCCATGT TTTACGCCAT 
GGAAACTCAA TGGACGCGTC CTTCTCCAAG GAAGTGCTCA ACTCCATTAC AGCCTTTTCA 
TCATTGGCTG TATCGACCGC TAATCATGCC GACTCCAGGG GTTCTCTGAT CAGCATCGAG 
TCGAACCCCA GCAACAGTGA CCGCAACAGT GAGAAGATGG ATGCCTGTGA AAAGTTTGCC 
AGGCCTCAGT CTCTTATTGG CTTTGGATCC CGCTTTGATA AACTGGATCA AGCTGAAACC 
AGAAGTCTGC TCATGTGTTT CCTGCACATT ATGAAGACCA TCTCAGAGGA GGTGATGGTT 
TCATACTGGC ATCGAGCGAT TCACCAGGAG ATCAGTGACT TCTTCAATAT TTTGGAATTA 
TGCCTTCAGC ACTTTCGTTT CCTTGGAAAG CGGCACATTG CCAGGAAGCT AGCGGCCGCA 
GTTAAGCTGG CTCAGGCCAC ACAGAACAAT GGCACCCTGA AGGGCTCTAA TGTGTCCTAC 
CACTCTCCAG GGCTCCTACC CCAATGGATT CTGTCTGCAC AGGACGGTCA CAGACACGCT 
CGCTCTCAGA CCATGCCCAT CATCAGGGGG AAAAACGCCC TCACAAACCC CAAACTGCTG 
CATATGATGG AGACAGACGT GGAGGCGAAC CTTTCTACTG AAGTAGCACT GACCGTGTTG 
GATGTTCTGG ACCTGTTTGT TCGACATCAT AAGAAACAGT TGCAGCACGA TGAAGGACAA 
AACTCTTTGA TGAAGAAAGT GTTCGACACG TACCTGCTGT TCTTCCAGAT CAACCAGTCC 
ACCACCACCC TCAGACACGT GTTCACAGCC CTGCGCCTCT TCATGCAGAA GTTTCCGTCC 
ACGTTTTTCC AGGGTAAAGC GGATGTGTGT GGCAGCCTGT GTTACGAGAT CCTGAAGTGC 
TGTAACCATC GCTCTAGCTC CACACAGACT GAAGCCGCCG CTCTGCTTTA TTTCCTCATG 
AGGAGGAACT TTGAGTTCAC GAAGGGCAAG TGCATCGTCC GCTCACACCT GCAGGTGATT 
AAAGCTGTGA GTCAGCTGAT CGCGGATGCA GGCATTGGCG GATCACGCTT CCAGCAGTCT 
CTGGCCATCA TCAATAACTT CGCCAATGGG GACACGCCAC TTAAAAACAC CACATTCCCA 
GCTGACGTAA AGGACCTGAC CAAGAGGATA CGCACCGTCC TGATGGCCAC GTCTCAAATG 
AAAGAGCATG AAAAAGACCC GGAAATGCTG GTGGACCTTC AGTACAGTCT GGCCAACTCC 
TACGCCAGCA CACCTGAGCT CAGACGCACC TGGCTGGAAA GCATGGCCAA AATACACGTC 
AGAAATGGAG ATCTGTCTGA GGCAGCCATG TGCTACATCC ACATCTCAGC CCTCATAGCA 
GAGTCTCTGA GGAGAAGAGG TTACTGGAAG TCTGAAAAGG GTCGGATGTC AAGTGTGAGC 
AAAGAGGGAG CCTGTGTAAA TAACTGTAGC CCTTTACTAA CTCCCCAAGA CGGAGAAACA 
TCATTCAGTA TGGGTTGGGC GGCATTTACG AATATCTCGC CTAACGTTAA AGAAGAGGGA 
GCAATGAAAG AAGACGCTGG CACTCAGGAT ACTCCATACA CTGAGGACAC GCTGGTGGAG 
CAGCTGGAGA TGTGTGTTGA TTATCTCTGG AAGTCTGAGA GATATGAACT TATTGCCGAG 
ATCAACAAAC CTGTTATTGT CGTCTTTGAG AAGAGAAGAG ATTTCAAGCG TCTATCAGAG 
CTGTACTATG ACATTCACCG CTCATACCTG AAGGTGACCG AAGTCGTGAA TTCTGAGAAA 
CGTCTGTTTG GTCGGTACTA TCGCGTGGCT TTCTACGGAC AGGGGTTTTT TGAGGAAGAG 
GAGAGTAAGG AGTTTATCTA CAAGGAGCCC AAACTCACAG GACTGTCCGA AATTTCTCAA 
AGACTCCTCA AACTCTACTC GGACAAGTTT GGAGCTGATA ACGTCAAAAT GATCCAGGAC 
TCCAACAAGG TGAATCCGAA AGACTTGGAC CCCAGATTTG CCTACATCCA GGTCACCTAT 
GTAGTGCCTT ATTTTAATGA GAAGGAACAG CTGGAGAAGA AAACCGACTT CGAGCGCCAT 
CACAACATCG ACCGATTTGT GTTTGAGACG CCCTTCACGC TTTCTGGAAA AAAACACGGA 
GACGTGGAGG AACAGTGCAA AAGGAAAACC ATCCTGACCA CGAGCTCTAG TTTCCCTTTC 
TTGAAGAAGC GTATAGAGGT GGTGGAGCAA CAGAGTACCG AGATGAACCC TATTGAGGTG 
GCGATAGACG AGATGAGCCG AAAAGTGTCT GAACTCAAAC AGCTGTGCAA TATGGAGGAG 
GTGGACATGA TCCGTCTGCA GCTCAAACTA CAGGGCAGCG TCAGCGTCAA GGTAAATGCA 
GGACCCATGG CCTACGCCCG AGCCTTCCTA GAGGAGAAAA ATGCTAAGAA ATACCCAGAC 
AACCAGGTCA AACTGCTGAA AGAGATCTTC AGACAGTTTG CAGAAGCGTG TGGACAGGCT 
CTGAGTGTGA ACGAGCGGCT AATAAAAGAG GATCAGTTTG AGTATCAGAG CGAGATGAAA 
GCTCACTATA AAGACATGCT CACGGAGCTC TCCGCCATTA TGAATGAGCA GATACCGTAC 
ACGAGACAAC CAGGAACAGA GCGACACAGT GCCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017206625.1
CDS	176..6694
Translation	MGCTTSLLQAVNFIMRRNHSRVFSDQQKQHLLETAPLEEGMVISEPHSTVVMQEKPKLIDPLDYEAVISELEAEFREDPLQDLLLFPDLDFTVSTLPVERRTVKSTVPEGADEHAECLLVRQACQYFNSQLHIVQYKYDEYGGDYRMLPRKLCKAEKLPSHSFEIDNEDADKDEDTTSLSSSKGGGGGAGSGGGGGIGVFKSGWLYKGNFNSTVNNSITVRSFKRRYFQLTQLSDNSYIMNFYKDEKISKEPKGCIFLDSCTGVVQNNRLRKHAFELKMNEVTYFVLAAENEQDMDDWINTLNRILQISPPDGPAQDRKSTDMSDSKNEISDIPQNNVPVHKNEETTENEIHPELAKYISETEESSRAVRAEERLNLFSLDPDIPVLRSPRTETGESSSVHPFDEKLGKRLMICCRSLNLTLQGCVNEADTEPTTNIEPFFVSVCLLDVREGRKVSADFHVDVNHELVRQMLNSSGNPGDQGSDVGNGAPLENGLTSPVEKKPEDCQLGPDMEKWLCFPKQAIFSITNPHTDIVMVARVEKVLMGNISSGAEPYIKNTDSSKTVHKMLKSNKQFCSKLGKYRMPFAWSVRSVFKDNQGTVDRESRFSPLFKQESNKISTEDLIKLITEYRRAEKASKLQTIPGNLEINVDCVPMECPNCVTSSYVALKPFEDCSQHAPTVEIDEFLQDSSKFAQPYRVYKNHIYIYPKHLKYDSQKSFAKARNIAVCVEFRSSDEEHAKPLKCIYGKPGGPVFTTTACTTVLHHSQNPDFYDEVKIELPTHLHEKHHILFSFYHVTCDINAKTSSKKKEALELPVGYAWLPLLKDGRISSQDFSIPVSCTLPAGYLHIKEPSSTKNGSDVKWVDGGKQIFKLSTLVLSTVYTQDPHLNRFFQQCQKREAEISQPPTSNFLNCLKGLLSMEKIHAIIRFLPVIFNQLFKVLTQNDSDEVTTSTTRVLIHILAKCHEENLDHYLHSYIKFVFKTEACGFRTIHEELAKGMTSDLKSNEQLVIRNVLKFSWFFLEIIVKSMAQHLLDSNKLKLPRPQRFPGTFQNRLETLIMTMSDHIYWKNKELAEETRSANMAIAFFVKRCFTLMDRGFTFKLISNYLNMITATDNKALCELKFEFIREVCNYEHFVPLSLPIPSARITDNASTEAQSTKVDFPEYSLTEDFCRKHFLSGLLLRELGLALQDEQDLRHIALACLKNLMAKHSLDARYATKEKQARIASLYLPLYGLILDNMPRFFLRDLFPICLTASDQGSRDDLSVGVGLGGQVTHVLRHGNSMDASFSKEVLNSITAFSSLAVSTANHADSRGSLISIESNPSNSDRNSEKMDACEKFARPQSLIGFGSRFDKLDQAETRSLLMCFLHIMKTISEEVMVSYWHRAIHQEISDFFNILELCLQHFRFLGKRHIARKLAAAVKLAQATQNNGTLKGSNVSYHSPGLLPQWILSAQDGHRHARSQTMPIIRGKNALTNPKLLHMMETDVEANLSTEVALTVLDVLDLFVRHHKKQLQHDEGQNSLMKKVFDTYLLFFQINQSTTTLRHVFTALRLFMQKFPSTFFQGKADVCGSLCYEILKCCNHRSSSTQTEAAALLYFLMRRNFEFTKGKCIVRSHLQVIKAVSQLIADAGIGGSRFQQSLAIINNFANGDTPLKNTTFPADVKDLTKRIRTVLMATSQMKEHEKDPEMLVDLQYSLANSYASTPELRRTWLESMAKIHVRNGDLSEAAMCYIHISALIAESLRRRGYWKSEKGRMSSVSKEGACVNNCSPLLTPQDGETSFSMGWAAFTNISPNVKEEGAMKEDAGTQDTPYTEDTLVEQLEMCVDYLWKSERYELIAEINKPVIVVFEKRRDFKRLSELYYDIHRSYLKVTEVVNSEKRLFGRYYRVAFYGQGFFEEEESKEFIYKEPKLTGLSEISQRLLKLYSDKFGADNVKMIQDSNKVNPKDLDPRFAYIQVTYVVPYFNEKEQLEKKTDFERHHNIDRFVFETPFTLSGKKHGDVEEQCKRKTILTTSSSFPFLKKRIEVVEQQSTEMNPIEVAIDEMSRKVSELKQLCNMEEVDMIRLQLKLQGSVSVKVNAGPMAYARAFLEEKNAKKYPDNQVKLLKEIFRQFAEACGQALSVNERLIKEDQFEYQSEMKAHYKDMLTELSAIMNEQIPYTRQPGTERHSAI

Target ORF information:

RefSeq Version	XM_017351136.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio dedicator of cytokinesis 10 (dock10), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351136.1

ORF Insert Sequence:

ATGGGCTGTA CCACGAGTCT ACTACAGGCT GTGAATTTTA TAATGAGGAG AAACCATTCA 
CGCGTCTTTT CTGACCAGCA GAAACAACAT TTGCTGGAAA CTGCACCGCT GGAGGAGGGG 
ATGGTCATCA GTGAACCACA CAGCACTGTC GTCATGCAGG AGAAGCCCAA ACTGATCGAC 
CCTCTGGACT ATGAAGCTGT GATTTCTGAA CTTGAAGCAG AATTCAGAGA AGATCCTCTT 
CAAGATCTCC TGTTATTCCC AGACCTTGAC TTCACTGTGT CCACACTTCC TGTAGAAAGG 
AGAACAGTGA AGTCTACTGT CCCAGAGGGA GCTGATGAAC ATGCTGAATG TCTGCTGGTC 
AGACAGGCCT GCCAATACTT CAACTCCCAG CTGCACATTG TTCAGTATAA ATATGATGAG 
TATGGAGGGG ACTACCGCAT GCTGCCCAGG AAGCTGTGTA AAGCAGAAAA GTTGCCTTCC 
CACTCTTTTG AGATTGACAA TGAAGATGCG GACAAAGATG AGGACACAAC ATCTTTGTCC 
TCATCTAAAG GAGGGGGCGG CGGTGCAGGC AGTGGAGGCG GAGGAGGAAT TGGTGTTTTT 
AAGTCCGGCT GGCTCTATAA AGGCAACTTC AACAGCACCG TCAACAACAG CATCACAGTT 
CGGTCCTTCA AGCGCCGATA TTTTCAGCTG ACTCAGCTCT CTGACAACTC TTACATCATG 
AACTTCTATA AAGATGAGAA AATTTCCAAG GAACCGAAAG GCTGTATTTT TCTGGACTCC 
TGCACCGGGG TGGTTCAGAA TAATCGACTG AGGAAACATG CGTTTGAGCT GAAAATGAAC 
GAAGTGACAT ATTTTGTCCT GGCTGCCGAG AATGAGCAAG ACATGGACGA CTGGATCAAC 
ACTTTAAATC GCATCCTGCA GATCAGCCCT CCAGACGGCC CGGCTCAGGA CCGCAAGAGT 
ACGGACATGA GCGACTCTAA AAACGAGATC AGTGATATAC CACAAAATAA CGTCCCTGTT 
CATAAAAACG AAGAAACAAC TGAAAATGAG ATCCACCCTG AACTAGCCAA GTATATTTCT 
GAGACAGAAG AAAGCTCGAG AGCTGTCAGA GCAGAGGAGA GACTGAACCT GTTCTCCCTG 
GACCCTGATA TTCCTGTTTT AAGGAGTCCT AGGACAGAAA CCGGGGAGTC TTCTTCTGTC 
CATCCATTTG ATGAGAAGCT TGGGAAGAGG CTGATGATCT GCTGTCGCTC ACTGAACCTC 
ACACTGCAAG GCTGCGTTAA TGAAGCGGAC ACCGAACCCA CCACTAATAT CGAGCCTTTC 
TTTGTGTCAG TGTGTCTGCT GGACGTACGT GAGGGCAGGA AGGTTTCCGC TGACTTCCAT 
GTGGACGTAA ACCATGAGTT AGTGCGGCAG ATGCTTAATA GCTCTGGTAA TCCGGGAGAT 
CAAGGCTCGG ATGTTGGTAA TGGTGCACCG CTGGAAAATG GACTCACTTC CCCGGTAGAA 
AAGAAACCAG AAGACTGTCA ACTAGGCCCA GACATGGAAA AGTGGCTATG CTTTCCCAAA 
CAGGCAATCT TTTCCATTAC GAACCCGCAC ACGGATATTG TGATGGTGGC ACGAGTGGAG 
AAAGTTCTGA TGGGAAATAT TTCCAGTGGT GCAGAGCCCT ACATCAAAAA CACCGATTCC 
AGCAAGACTG TACACAAGAT GCTGAAATCC AATAAGCAGT TCTGTAGCAA ACTAGGCAAG 
TACCGAATGC CTTTCGCCTG GTCTGTAAGG TCAGTGTTTA AGGACAACCA GGGCACAGTG 
GACAGGGAGT CTCGCTTCTC TCCTCTTTTC AAACAGGAAA GCAACAAGAT TTCCACCGAA 
GACCTCATCA AGCTCATCAC CGAGTACAGG AGGGCGGAGA AGGCCAGTAA ACTCCAGACC 
ATCCCTGGAA ACCTTGAAAT AAATGTGGAC TGTGTTCCCA TGGAATGTCC CAATTGTGTG 
ACGTCCTCAT ATGTTGCCCT GAAGCCGTTT GAGGACTGCA GCCAACATGC TCCTACGGTG 
GAAATCGATG AGTTCCTGCA GGATTCGTCA AAGTTCGCTC AACCTTACAG AGTCTACAAG 
AACCACATCT ACATCTACCC TAAACACCTC AAGTATGACA GCCAGAAGAG CTTTGCTAAG 
GCTCGAAATA TTGCAGTTTG TGTGGAGTTT CGAAGCTCCG ATGAAGAACA TGCCAAGCCG 
CTTAAGTGCA TCTATGGAAA GCCCGGAGGC CCAGTTTTTA CCACGACTGC CTGTACCACA 
GTTCTGCACC ATTCACAGAA CCCAGACTTT TATGATGAGG TCAAGATTGA GTTGCCCACA 
CATCTTCATG AGAAACACCA TATACTCTTC TCCTTCTATC ATGTGACCTG TGACATCAAT 
GCCAAGACCA GCTCTAAGAA GAAAGAGGCT CTTGAATTGC CAGTGGGTTA TGCATGGCTG 
CCTCTTCTAA AGGATGGACG CATTTCCTCT CAGGACTTCA GCATTCCTGT CTCCTGCACT 
TTGCCTGCTG GCTATTTACA CATTAAAGAG CCTTCGTCCA CCAAGAATGG CTCTGATGTG 
AAGTGGGTCG ATGGAGGGAA GCAGATCTTC AAATTGTCCA CTTTGGTGCT TTCTACAGTG 
TATACTCAGG ATCCCCATCT AAACCGCTTC TTCCAGCAGT GCCAAAAACG AGAGGCAGAA 
ATCTCTCAAC CTCCTACCTC AAACTTCCTC AACTGCCTTA AAGGGCTCTT GAGCATGGAG 
AAGATTCATG CCATAATTCG CTTCCTTCCT GTTATCTTCA ACCAGCTCTT TAAAGTGCTT 
ACGCAAAACG ACTCTGATGA AGTCACCACC AGCACCACCA GGGTCCTGAT ACACATCTTA 
GCCAAGTGCC ACGAGGAGAA CCTGGACCAC TATTTGCACT CCTACATTAA GTTTGTCTTT 
AAGACTGAAG CGTGTGGCTT CAGAACCATT CATGAAGAGC TGGCCAAGGG AATGACCTCT 
GACCTGAAGA GCAATGAACA GTTAGTCATA CGCAACGTCC TGAAGTTCTC GTGGTTCTTT 
CTCGAGATCA TAGTGAAGTC AATGGCTCAG CATCTGCTGG ACTCAAACAA GCTCAAGCTG 
CCGCGACCGC AACGATTTCC TGGCACTTTC CAAAACCGTT TGGAGACGTT GATCATGACC 
ATGTCGGATC ACATTTACTG GAAGAACAAA GAGCTGGCGG AGGAGACGCG GAGTGCAAAT 
ATGGCCATTG CGTTTTTTGT GAAGCGGTGC TTCACCTTGA TGGACAGAGG TTTCACCTTT 
AAACTGATCA GCAACTACCT GAACATGATC ACAGCCACTG ACAATAAGGC GCTGTGTGAG 
CTGAAGTTTG AGTTCATAAG GGAAGTGTGT AACTATGAGC ACTTTGTCCC TTTGAGTCTT 
CCCATCCCGT CTGCACGAAT CACAGACAAT GCATCAACAG AAGCCCAAAG TACTAAGGTG 
GACTTCCCAG AGTACAGTTT GACAGAGGAT TTCTGCAGGA AGCATTTTCT GTCTGGATTG 
TTGTTGCGAG AGCTGGGACT CGCTTTGCAG GATGAGCAAG ATCTGAGACA CATCGCTCTG 
GCCTGCCTGA AGAACCTGAT GGCCAAACAC TCGCTGGACG CTCGATACGC CACAAAGGAG 
AAACAGGCTC GGATAGCTTC TCTTTACCTG CCGCTTTATG GCCTGATCCT GGACAACATG 
CCTCGGTTTT TCCTCAGAGA TCTGTTTCCA ATCTGCCTGA CAGCGAGTGA CCAGGGATCC 
AGAGATGATC TCAGTGTTGG AGTAGGTTTG GGTGGTCAGG TGACCCATGT TTTACGCCAT 
GGAAACTCAA TGGACGCGTC CTTCTCCAAG GAAGTGCTCA ACTCCATTAC AGCCTTTTCA 
TCATTGGCTG TATCGACCGC TAATCATGCC GACTCCAGGG GTTCTCTGAT CAGCATCGAG 
TCGAACCCCA GCAACAGTGA CCGCAACAGT GAGAAGATGG ATGCCTGTGA AAAGTTTGCC 
AGGCCTCAGT CTCTTATTGG CTTTGGATCC CGCTTTGATA AACTGGATCA AGCTGAAACC 
AGAAGTCTGC TCATGTGTTT CCTGCACATT ATGAAGACCA TCTCAGAGGA GGTGATGGTT 
TCATACTGGC ATCGAGCGAT TCACCAGGAG ATCAGTGACT TCTTCAATAT TTTGGAATTA 
TGCCTTCAGC ACTTTCGTTT CCTTGGAAAG CGGCACATTG CCAGGAAGCT AGCGGCCGCA 
GTTAAGCTGG CTCAGGCCAC ACAGAACAAT GGCACCCTGA AGGGCTCTAA TGTGTCCTAC 
CACTCTCCAG GGCTCCTACC CCAATGGATT CTGTCTGCAC AGGACGGTCA CAGACACGCT 
CGCTCTCAGA CCATGCCCAT CATCAGGGGG AAAAACGCCC TCACAAACCC CAAACTGCTG 
CATATGATGG AGACAGACGT GGAGGCGAAC CTTTCTACTG AAGTAGCACT GACCGTGTTG 
GATGTTCTGG ACCTGTTTGT TCGACATCAT AAGAAACAGT TGCAGCACGA TGAAGGACAA 
AACTCTTTGA TGAAGAAAGT GTTCGACACG TACCTGCTGT TCTTCCAGAT CAACCAGTCC 
ACCACCACCC TCAGACACGT GTTCACAGCC CTGCGCCTCT TCATGCAGAA GTTTCCGTCC 
ACGTTTTTCC AGGGTAAAGC GGATGTGTGT GGCAGCCTGT GTTACGAGAT CCTGAAGTGC 
TGTAACCATC GCTCTAGCTC CACACAGACT GAAGCCGCCG CTCTGCTTTA TTTCCTCATG 
AGGAGGAACT TTGAGTTCAC GAAGGGCAAG TGCATCGTCC GCTCACACCT GCAGGTGATT 
AAAGCTGTGA GTCAGCTGAT CGCGGATGCA GGCATTGGCG GATCACGCTT CCAGCAGTCT 
CTGGCCATCA TCAATAACTT CGCCAATGGG GACACGCCAC TTAAAAACAC CACATTCCCA 
GCTGACGTAA AGGACCTGAC CAAGAGGATA CGCACCGTCC TGATGGCCAC GTCTCAAATG 
AAAGAGCATG AAAAAGACCC GGAAATGCTG GTGGACCTTC AGTACAGTCT GGCCAACTCC 
TACGCCAGCA CACCTGAGCT CAGACGCACC TGGCTGGAAA GCATGGCCAA AATACACGTC 
AGAAATGGAG ATCTGTCTGA GGCAGCCATG TGCTACATCC ACATCTCAGC CCTCATAGCA 
GAGTCTCTGA GGAGAAGAGG TTACTGGAAG TCTGAAAAGG GTCGGATGTC AAGTGTGAGC 
AAAGAGGGAG CCTGTGTAAA TAACTGTAGC CCTTTACTAA CTCCCCAAGA CGGAGAAACA 
TCATTCAGTA TGGGTTGGGC GGCATTTACG AATATCTCGC CTAACGTTAA AGAAGAGGGA 
GCAATGAAAG AAGACGCTGG CACTCAGGAT ACTCCATACA CTGAGGACAC GCTGGTGGAG 
CAGCTGGAGA TGTGTGTTGA TTATCTCTGG AAGTCTGAGA GATATGAACT TATTGCCGAG 
ATCAACAAAC CTGTTATTGT CGTCTTTGAG AAGAGAAGAG ATTTCAAGCG TCTATCAGAG 
CTGTACTATG ACATTCACCG CTCATACCTG AAGGTGACCG AAGTCGTGAA TTCTGAGAAA 
CGTCTGTTTG GTCGGTACTA TCGCGTGGCT TTCTACGGAC AGGGGTTTTT TGAGGAAGAG 
GAGAGTAAGG AGTTTATCTA CAAGGAGCCC AAACTCACAG GACTGTCCGA AATTTCTCAA 
AGACTCCTCA AACTCTACTC GGACAAGTTT GGAGCTGATA ACGTCAAAAT GATCCAGGAC 
TCCAACAAGG TGAATCCGAA AGACTTGGAC CCCAGATTTG CCTACATCCA GGTCACCTAT 
GTAGTGCCTT ATTTTAATGA GAAGGAACAG CTGGAGAAGA AAACCGACTT CGAGCGCCAT 
CACAACATCG ACCGATTTGT GTTTGAGACG CCCTTCACGC TTTCTGGAAA AAAACACGGA 
GACGTGGAGG AACAGTGCAA AAGGAAAACC ATCCTGACCA CGAGCTCTAG TTTCCCTTTC 
TTGAAGAAGC GTATAGAGGT GGTGGAGCAA CAGAGTACCG AGATGAACCC TATTGAGGTG 
GCGATAGACG AGATGAGCCG AAAAGTGTCT GAACTCAAAC AGCTGTGCAA TATGGAGGAG 
GTGGACATGA TCCGTCTGCA GCTCAAACTA CAGGGCAGCG TCAGCGTCAA GGTAAATGCA 
GGACCCATGG CCTACGCCCG AGCCTTCCTA GAGGAGAAAA ATGCTAAGAA ATACCCAGAC 
AACCAGGTCA AACTGCTGAA AGAGATCTTC AGACAGTTTG CAGAAGCGTG TGGACAGGCT 
CTGAGTGTGA ACGAGCGGCT AATAAAAGAG GATCAGTTTG AGTATCAGAG CGAGATGAAA 
GCTCACTATA AAGACATGCT CACGGAGCTC TCCGCCATTA TGAATGAGCA GATACCGTAC 
ACGAGACAAC CAGGAACAGA GCGACACAGT GCCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOCK10 ( XM_001341012.8 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_001341048.5 Danio rerio dedicator of cytokinesis 10 (dock10), transcript variant X1, mRNA.

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CloneID	ODa20115
Clone ID Related Accession (Same CDS sequence)	XM_001341012.8 , XM_001341012.7
Accession Version	XM_001341012.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	dedicator of cytokinesis protein 10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001341012.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTGTA CCACGAGTCT ACTACAGGCT GTGAATTTTA TAATGAGGAG AAACCATTCA 
CGCGTCTTTT CTGACCAGCA GAAACAACAT TTGCTGGAAA CTGCACCGCT GGAGGAGGGG 
ATGGTCATCA GTGAACCACA CAGCACTGTC GTCATGCAGG AGAAGCCCAA ACTGATCGAC 
CCTCTGGACT ATGAAGCTGT GATTTCTGAA CTTGAAGCAG AATTCAGAGA AGATCCTCTT 
CAAGATCTCC TGTTATTCCC AGACCTTGAC TTCACTGTGT CCACACTTCC TGTAGAAAGG 
AGAACAGTGA AGTCTACTGT CCCAGAGGGA GCTGATGAAC ATGCTGAATG TCTGCTGGTC 
AGACAGGCCT GCCAATACTT CAACTCCCAG CTGCACATTG TTCAGTATAA ATATGATGAG 
TATGGAGGGG ACTACCGCAT GCTGCCCAGG AAGCTGTGTA AAGCAGAAAA GTTGCCTTCC 
CACTCTTTTG AGATTGACAA TGAAGATGCG GACAAAGATG AGGACACAAC ATCTTTGTCC 
TCATCTAAAG GAGGGGGCGG CGGTGCAGGC AGTGGAGGCG GAGGAGGAAT TGGTGTTTTT 
AAGTCCGGCT GGCTCTATAA AGGCAACTTC AACAGCACCG TCAACAACAG CATCACAGTT 
CGGTCCTTCA AGCGCCGATA TTTTCAGCTG ACTCAGCTCT CTGACAACTC TTACATCATG 
AACTTCTATA AAGATGAGAA AATTTCCAAG GAACCGAAAG GCTGTATTTT TCTGGACTCC 
TGCACCGGGG TGGTTCAGAA TAATCGACTG AGGAAACATG CGTTTGAGCT GAAAATGAAC 
GAAGTGACAT ATTTTGTCCT GGCTGCCGAG AATGAGCAAG ACATGGACGA CTGGATCAAC 
ACTTTAAATC GCATCCTGCA GATCAGCCCT CCAGACGGCC CGGCTCAGGA CCGCAAGAGT 
ACGGACATGA GCGACTCTAA AAACGAGATC AGTGATATAC CACAAAATAA CGTCCCTGTT 
CATAAAAACG AAGAAACAAC TGAAAATGAG ATCCACCCTG AACTAGCCAA GTATATTTCT 
GAGACAGAAG AAAGCTCGAG AGCTGTCAGA GCAGAGGAGA GACTGAACCT GTTCTCCCTG 
GACCCTGATA TTCCTGTTTT AAGGAGTCCT AGGACAGAAA CCGGGGAGTC TTCTTCTGTC 
CATCCATTTG ATGAGAAGCT TGGGAAGAGG CTGATGATCT GCTGTCGCTC ACTGAACCTC 
ACACTGCAAG GCTGCGTTAA TGAAGCGGAC ACCGAACCCA CCACTAATAT CGAGCCTTTC 
TTTGTGTCAG TGTGTCTGCT GGACGTACGT GAGGGCAGGA AGGTTTCCGC TGACTTCCAT 
GTGGACGTAA ACCATGAGTT AGTGCGGCAG ATGCTTAATA GCTCTGGTAA TCCGGGAGAT 
CAAGGCTCGG ATGTTGGTAA TGGTGCACCG CTGGAAAATG GACTCACTTC CCCGGTAGAA 
AAGAAACCAG AAGACTGTCA ACTAGGCCCA GACATGGAAA AGTGGCTATG CTTTCCCAAA 
CAGGCAATCT TTTCCATTAC GAACCCGCAC ACGGATATTG TGATGGTGGC ACGAGTGGAG 
AAAGTTCTGA TGGGAAATAT TTCCAGTGGT GCAGAGCCCT ACATCAAAAA CACCGATTCC 
AGCAAGACTG TACACAAGAT GCTGAAATCC AATAAGCAGT TCTGTAGCAA ACTAGGCAAG 
TACCGAATGC CTTTCGCCTG GTCTGTAAGG TCAGTGTTTA AGGACAACCA GGGCACAGTG 
GACAGGGAGT CTCGCTTCTC TCCTCTTTTC AAACAGGAAA GCAACAAGAT TTCCACCGAA 
GACCTCATCA AGCTCATCAC CGAGTACAGG AGGGCGGAGA AGGCCAGTAA ACTCCAGACC 
ATCCCTGGAA ACCTTGAAAT AAATGTGGAC TGTGTTCCCA TGGAATGTCC CAATTGTGTG 
ACGTCCTCAT ATGTTGCCCT GAAGCCGTTT GAGGACTGCA GCCAACATGC TCCTACGGTG 
GAAATCGATG AGTTCCTGCA GGATTCGTCA AAGTTCGCTC AACCTTACAG AGTCTACAAG 
AACCACATCT ACATCTACCC TAAACACCTC AAGTATGACA GCCAGAAGAG CTTTGCTAAG 
GCTCGAAATA TTGCAGTTTG TGTGGAGTTT CGAAGCTCCG ATGAAGAACA TGCCAAGCCG 
CTTAAGTGCA TCTATGGAAA GCCCGGAGGC CCAGTTTTTA CCACGACTGC CTGTACCACA 
GTTCTGCACC ATTCACAGAA CCCAGACTTT TATGATGAGG TCAAGATTGA GTTGCCCACA 
CATCTTCATG AGAAACACCA TATACTCTTC TCCTTCTATC ATGTGACCTG TGACATCAAT 
GCCAAGACCA GCTCTAAGAA GAAAGAGGCT CTTGAATTGC CAGTGGGTTA TGCATGGCTG 
CCTCTTCTAA AGGATGGACG CATTTCCTCT CAGGACTTCA GCATTCCTGT CTCCTGCACT 
TTGCCTGCTG GCTATTTACA CATTAAAGAG CCTTCGTCCA CCAAGAATGG CTCTGATGTG 
AAGTGGGTCG ATGGAGGGAA GCAGATCTTC AAATTGTCCA CTTTGGTGCT TTCTACAGTG 
TATACTCAGG ATCCCCATCT AAACCGCTTC TTCCAGCAGT GCCAAAAACG AGAGGCAGAA 
ATCTCTCAAC CTCCTACCTC AAACTTCCTC AACTGCCTTA AAGGGCTCTT GAGCATGGAG 
AAGATTCATG CCATAATTCG CTTCCTTCCT GTTATCTTCA ACCAGCTCTT TAAAGTGCTT 
ACGCAAAACG ACTCTGATGA AGTCACCACC AGCACCACCA GGGTCCTGAT ACACATCTTA 
GCCAAGTGCC ACGAGGAGAA CCTGGACCAC TATTTGCACT CCTACATTAA GTTTGTCTTT 
AAGACTGAAG CGTGTGGCTT CAGAACCATT CATGAAGAGC TGGCCAAGGG AATGACCTCT 
GACCTGAAGA GCAATGAACA GTTAGTCATA CGCAACGTCC TGAAGTTCTC GTGGTTCTTT 
CTCGAGATCA TAGTGAAGTC AATGGCTCAG CATCTGCTGG ACTCAAACAA GCTCAAGCTG 
CCGCGACCGC AACGATTTCC TGGCACTTTC CAAAACCGTT TGGAGACGTT GATCATGACC 
ATGTCGGATC ACATTTACTG GAAGAACAAA GAGCTGGCGG AGGAGACGCG GAGTGCAAAT 
ATGGCCATTG CGTTTTTTGT GAAGCGGTGC TTCACCTTGA TGGACAGAGG TTTCACCTTT 
AAACTGATCA GCAACTACCT GAACATGATC ACAGCCACTG ACAATAAGGC GCTGTGTGAG 
CTGAAGTTTG AGTTCATAAG GGAAGTGTGT AACTATGAGC ACTTTGTCCC TTTGAGTCTT 
CCCATCCCGT CTGCACGAAT CACAGACAAT GCATCAACAG AAGCCCAAAG TACTAAGGTG 
GACTTCCCAG AGTACAGTTT GACAGAGGAT TTCTGCAGGA AGCATTTTCT GTCTGGATTG 
TTGTTGCGAG AGCTGGGACT CGCTTTGCAG GATGAGCAAG ATCTGAGACA CATCGCTCTG 
GCCTGCCTGA AGAACCTGAT GGCCAAACAC TCGCTGGACG CTCGATACGC CACAAAGGAG 
AAACAGGCTC GGATAGCTTC TCTTTACCTG CCGCTTTATG GCCTGATCCT GGACAACATG 
CCTCGGTTTT TCCTCAGAGA TCTGTTTCCA ATCTGCCTGA CAGCGAGTGA CCAGGGATCC 
AGAGATGATC TCAGTGTTGG AGTAGGTTTG GGTGGTCAGG TGACCCATGT TTTACGCCAT 
GGAAACTCAA TGGACGCGTC CTTCTCCAAG GAAGTGCTCA ACTCCATTAC AGCCTTTTCA 
TCATTGGCTG TATCGACCGC TAATCATGCC GACTCCAGGG GTTCTCTGAT CAGCATCGAG 
TCGAACCCCA GCAACAGTGA CCGCAACAGT GAGAAGATGG ATGCCTGTGA AAAGTTTGCC 
AGGCCTCAGT CTCTTATTGG CTTTGGATCC CGCTTTGATA AACTGGATCA AGCTGAAACC 
AGAAGTCTGC TCATGTGTTT CCTGCACATT ATGAAGACCA TCTCAGAGGA GGTGATGGTT 
TCATACTGGC ATCGAGCGAT TCACCAGGAG ATCAGTGACT TCTTCAATAT TTTGGAATTA 
TGCCTTCAGC ACTTTCGTTT CCTTGGAAAG CGGCACATTG CCAGGAAGCT AGCGGCCGCA 
GTTAAGCTGG CTCAGGCCAC ACAGAACAAT GGCACCCTGA AGGGCTCTAA TGTGTCCTAC 
CACTCTCCAG GGCTCCTACC CCAATGGATT CTGTCTGCAC AGGACGGTCA CAGACACGCT 
CGCTCTCAGA CCATGCCCAT CATCAGGGGG AAAAACGCCC TCACAAACCC CAAACTGCTG 
CATATGATGG AGACAGATGG AAATGTTGCT GAAGGAGAAA ACTTTAACCC TACAGACGTG 
GAGGCGAACC TTTCTACTGA AGTAGCACTG ACCGTGTTGG ATGTTCTGGA CCTGTTTGTT 
CGACATCATA AGAAACAGTT GCAGCACGAT GAAGGACAAA ACTCTTTGAT GAAGAAAGTG 
TTCGACACGT ACCTGCTGTT CTTCCAGATC AACCAGTCCA CCACCACCCT CAGACACGTG 
TTCACAGCCC TGCGCCTCTT CATGCAGAAG TTTCCGTCCA CGTTTTTCCA GGGTAAAGCG 
GATGTGTGTG GCAGCCTGTG TTACGAGATC CTGAAGTGCT GTAACCATCG CTCTAGCTCC 
ACACAGACTG AAGCCGCCGC TCTGCTTTAT TTCCTCATGA GGAGGAACTT TGAGTTCACG 
AAGGGCAAGT GCATCGTCCG CTCACACCTG CAGGTGATTA AAGCTGTGAG TCAGCTGATC 
GCGGATGCAG GCATTGGCGG ATCACGCTTC CAGCAGTCTC TGGCCATCAT CAATAACTTC 
GCCAATGGGG ACACGCCACT TAAAAACACC ACATTCCCAG CTGACGTAAA GGACCTGACC 
AAGAGGATAC GCACCGTCCT GATGGCCACG TCTCAAATGA AAGAGCATGA AAAAGACCCG 
GAAATGCTGG TGGACCTTCA GTACAGTCTG GCCAACTCCT ACGCCAGCAC ACCTGAGCTC 
AGACGCACCT GGCTGGAAAG CATGGCCAAA ATACACGTCA GAAATGGAGA TCTGTCTGAG 
GCAGCCATGT GCTACATCCA CATCTCAGCC CTCATAGCAG AGTCTCTGAG GAGAAGAGGT 
TACTGGAAGT CTGAAAAGGG TCGGATGTCA AGTGTGAGCA AAGAGGGAGC CTGTGTAAAT 
AACTGTAGCC CTTTACTAAC TCCCCAAGAC GGAGAAACAT CATTCAGTAT GGGTTGGGCG 
GCATTTACGA ATATCTCGCC TAACGTTAAA GAAGAGGGAG CAATGAAAGA AGACGCTGGC 
ACTCAGGATA CTCCATACAC TGAGGACACG CTGGTGGAGC AGCTGGAGAT GTGTGTTGAT 
TATCTCTGGA AGTCTGAGAG ATATGAACTT ATTGCCGAGA TCAACAAACC TGTTATTGTC 
GTCTTTGAGA AGAGAAGAGA TTTCAAGCGT CTATCAGAGC TGTACTATGA CATTCACCGC 
TCATACCTGA AGGTGACCGA AGTCGTGAAT TCTGAGAAAC GTCTGTTTGG TCGGTACTAT 
CGCGTGGCTT TCTACGGACA GGGGTTTTTT GAGGAAGAGG AGAGTAAGGA GTTTATCTAC 
AAGGAGCCCA AACTCACAGG ACTGTCCGAA ATTTCTCAAA GACTCCTCAA ACTCTACTCG 
GACAAGTTTG GAGCTGATAA CGTCAAAATG ATCCAGGACT CCAACAAGGT GAATCCGAAA 
GACTTGGACC CCAGATTTGC CTACATCCAG GTCACCTATG TAGTGCCTTA TTTTAATGAG 
AAGGAACAGC TGGAGAAGAA AACCGACTTC GAGCGCCATC ACAACATCGA CCGATTTGTG 
TTTGAGACGC CCTTCACGCT TTCTGGAAAA AAACACGGAG ACGTGGAGGA ACAGTGCAAA 
AGGAAAACCA TCCTGACCAC GAGCTCTAGT TTCCCTTTCT TGAAGAAGCG TATAGAGGTG 
GTGGAGCAAC AGAGTACCGA GATGAACCCT ATTGAGGTGG CGATAGACGA GATGAGCCGA 
AAAGTGTCTG AACTCAAACA GCTGTGCAAT ATGGAGGAGG TGGACATGAT CCGTCTGCAG 
CTCAAACTAC AGGGCAGCGT CAGCGTCAAG GTAAATGCAG GACCCATGGC CTACGCCCGA 
GCCTTCCTAG AGGAGAAAAA TGCTAAGAAA TACCCAGACA ACCAGGTCAA ACTGCTGAAA 
GAGATCTTCA GACAGTTTGC AGAAGCGTGT GGACAGGCTC TGAGTGTGAA CGAGCGGCTA 
ATAAAAGAGG ATCAGTTTGA GTATCAGAGC GAGATGAAAG CTCACTATAA AGACATGCTC 
ACGGAGCTCT CCGCCATTAT GAATGAGCAG ATACCGTACA CGAGACAACC AGGAACAGAG 
CGACACAGTG CCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001341048.5
CDS	273..6830
Translation	MGCTTSLLQAVNFIMRRNHSRVFSDQQKQHLLETAPLEEGMVISEPHSTVVMQEKPKLIDPLDYEAVISELEAEFREDPLQDLLLFPDLDFTVSTLPVERRTVKSTVPEGADEHAECLLVRQACQYFNSQLHIVQYKYDEYGGDYRMLPRKLCKAEKLPSHSFEIDNEDADKDEDTTSLSSSKGGGGGAGSGGGGGIGVFKSGWLYKGNFNSTVNNSITVRSFKRRYFQLTQLSDNSYIMNFYKDEKISKEPKGCIFLDSCTGVVQNNRLRKHAFELKMNEVTYFVLAAENEQDMDDWINTLNRILQISPPDGPAQDRKSTDMSDSKNEISDIPQNNVPVHKNEETTENEIHPELAKYISETEESSRAVRAEERLNLFSLDPDIPVLRSPRTETGESSSVHPFDEKLGKRLMICCRSLNLTLQGCVNEADTEPTTNIEPFFVSVCLLDVREGRKVSADFHVDVNHELVRQMLNSSGNPGDQGSDVGNGAPLENGLTSPVEKKPEDCQLGPDMEKWLCFPKQAIFSITNPHTDIVMVARVEKVLMGNISSGAEPYIKNTDSSKTVHKMLKSNKQFCSKLGKYRMPFAWSVRSVFKDNQGTVDRESRFSPLFKQESNKISTEDLIKLITEYRRAEKASKLQTIPGNLEINVDCVPMECPNCVTSSYVALKPFEDCSQHAPTVEIDEFLQDSSKFAQPYRVYKNHIYIYPKHLKYDSQKSFAKARNIAVCVEFRSSDEEHAKPLKCIYGKPGGPVFTTTACTTVLHHSQNPDFYDEVKIELPTHLHEKHHILFSFYHVTCDINAKTSSKKKEALELPVGYAWLPLLKDGRISSQDFSIPVSCTLPAGYLHIKEPSSTKNGSDVKWVDGGKQIFKLSTLVLSTVYTQDPHLNRFFQQCQKREAEISQPPTSNFLNCLKGLLSMEKIHAIIRFLPVIFNQLFKVLTQNDSDEVTTSTTRVLIHILAKCHEENLDHYLHSYIKFVFKTEACGFRTIHEELAKGMTSDLKSNEQLVIRNVLKFSWFFLEIIVKSMAQHLLDSNKLKLPRPQRFPGTFQNRLETLIMTMSDHIYWKNKELAEETRSANMAIAFFVKRCFTLMDRGFTFKLISNYLNMITATDNKALCELKFEFIREVCNYEHFVPLSLPIPSARITDNASTEAQSTKVDFPEYSLTEDFCRKHFLSGLLLRELGLALQDEQDLRHIALACLKNLMAKHSLDARYATKEKQARIASLYLPLYGLILDNMPRFFLRDLFPICLTASDQGSRDDLSVGVGLGGQVTHVLRHGNSMDASFSKEVLNSITAFSSLAVSTANHADSRGSLISIESNPSNSDRNSEKMDACEKFARPQSLIGFGSRFDKLDQAETRSLLMCFLHIMKTISEEVMVSYWHRAIHQEISDFFNILELCLQHFRFLGKRHIARKLAAAVKLAQATQNNGTLKGSNVSYHSPGLLPQWILSAQDGHRHARSQTMPIIRGKNALTNPKLLHMMETDGNVAEGENFNPTDVEANLSTEVALTVLDVLDLFVRHHKKQLQHDEGQNSLMKKVFDTYLLFFQINQSTTTLRHVFTALRLFMQKFPSTFFQGKADVCGSLCYEILKCCNHRSSSTQTEAAALLYFLMRRNFEFTKGKCIVRSHLQVIKAVSQLIADAGIGGSRFQQSLAIINNFANGDTPLKNTTFPADVKDLTKRIRTVLMATSQMKEHEKDPEMLVDLQYSLANSYASTPELRRTWLESMAKIHVRNGDLSEAAMCYIHISALIAESLRRRGYWKSEKGRMSSVSKEGACVNNCSPLLTPQDGETSFSMGWAAFTNISPNVKEEGAMKEDAGTQDTPYTEDTLVEQLEMCVDYLWKSERYELIAEINKPVIVVFEKRRDFKRLSELYYDIHRSYLKVTEVVNSEKRLFGRYYRVAFYGQGFFEEEESKEFIYKEPKLTGLSEISQRLLKLYSDKFGADNVKMIQDSNKVNPKDLDPRFAYIQVTYVVPYFNEKEQLEKKTDFERHHNIDRFVFETPFTLSGKKHGDVEEQCKRKTILTTSSSFPFLKKRIEVVEQQSTEMNPIEVAIDEMSRKVSELKQLCNMEEVDMIRLQLKLQGSVSVKVNAGPMAYARAFLEEKNAKKYPDNQVKLLKEIFRQFAEACGQALSVNERLIKEDQFEYQSEMKAHYKDMLTELSAIMNEQIPYTRQPGTERHSAI

Target ORF information:

RefSeq Version	XM_001341012.8
Organism	Danio rerio(zebrafish)
Definition	Danio rerio dedicator of cytokinesis 10 (dock10), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001341012.8

ORF Insert Sequence:

ATGGGCTGTA CCACGAGTCT ACTACAGGCT GTGAATTTTA TAATGAGGAG AAACCATTCA 
CGCGTCTTTT CTGACCAGCA GAAACAACAT TTGCTGGAAA CTGCACCGCT GGAGGAGGGG 
ATGGTCATCA GTGAACCACA CAGCACTGTC GTCATGCAGG AGAAGCCCAA ACTGATCGAC 
CCTCTGGACT ATGAAGCTGT GATTTCTGAA CTTGAAGCAG AATTCAGAGA AGATCCTCTT 
CAAGATCTCC TGTTATTCCC AGACCTTGAC TTCACTGTGT CCACACTTCC TGTAGAAAGG 
AGAACAGTGA AGTCTACTGT CCCAGAGGGA GCTGATGAAC ATGCTGAATG TCTGCTGGTC 
AGACAGGCCT GCCAATACTT CAACTCCCAG CTGCACATTG TTCAGTATAA ATATGATGAG 
TATGGAGGGG ACTACCGCAT GCTGCCCAGG AAGCTGTGTA AAGCAGAAAA GTTGCCTTCC 
CACTCTTTTG AGATTGACAA TGAAGATGCG GACAAAGATG AGGACACAAC ATCTTTGTCC 
TCATCTAAAG GAGGGGGCGG CGGTGCAGGC AGTGGAGGCG GAGGAGGAAT TGGTGTTTTT 
AAGTCCGGCT GGCTCTATAA AGGCAACTTC AACAGCACCG TCAACAACAG CATCACAGTT 
CGGTCCTTCA AGCGCCGATA TTTTCAGCTG ACTCAGCTCT CTGACAACTC TTACATCATG 
AACTTCTATA AAGATGAGAA AATTTCCAAG GAACCGAAAG GCTGTATTTT TCTGGACTCC 
TGCACCGGGG TGGTTCAGAA TAATCGACTG AGGAAACATG CGTTTGAGCT GAAAATGAAC 
GAAGTGACAT ATTTTGTCCT GGCTGCCGAG AATGAGCAAG ACATGGACGA CTGGATCAAC 
ACTTTAAATC GCATCCTGCA GATCAGCCCT CCAGACGGCC CGGCTCAGGA CCGCAAGAGT 
ACGGACATGA GCGACTCTAA AAACGAGATC AGTGATATAC CACAAAATAA CGTCCCTGTT 
CATAAAAACG AAGAAACAAC TGAAAATGAG ATCCACCCTG AACTAGCCAA GTATATTTCT 
GAGACAGAAG AAAGCTCGAG AGCTGTCAGA GCAGAGGAGA GACTGAACCT GTTCTCCCTG 
GACCCTGATA TTCCTGTTTT AAGGAGTCCT AGGACAGAAA CCGGGGAGTC TTCTTCTGTC 
CATCCATTTG ATGAGAAGCT TGGGAAGAGG CTGATGATCT GCTGTCGCTC ACTGAACCTC 
ACACTGCAAG GCTGCGTTAA TGAAGCGGAC ACCGAACCCA CCACTAATAT CGAGCCTTTC 
TTTGTGTCAG TGTGTCTGCT GGACGTACGT GAGGGCAGGA AGGTTTCCGC TGACTTCCAT 
GTGGACGTAA ACCATGAGTT AGTGCGGCAG ATGCTTAATA GCTCTGGTAA TCCGGGAGAT 
CAAGGCTCGG ATGTTGGTAA TGGTGCACCG CTGGAAAATG GACTCACTTC CCCGGTAGAA 
AAGAAACCAG AAGACTGTCA ACTAGGCCCA GACATGGAAA AGTGGCTATG CTTTCCCAAA 
CAGGCAATCT TTTCCATTAC GAACCCGCAC ACGGATATTG TGATGGTGGC ACGAGTGGAG 
AAAGTTCTGA TGGGAAATAT TTCCAGTGGT GCAGAGCCCT ACATCAAAAA CACCGATTCC 
AGCAAGACTG TACACAAGAT GCTGAAATCC AATAAGCAGT TCTGTAGCAA ACTAGGCAAG 
TACCGAATGC CTTTCGCCTG GTCTGTAAGG TCAGTGTTTA AGGACAACCA GGGCACAGTG 
GACAGGGAGT CTCGCTTCTC TCCTCTTTTC AAACAGGAAA GCAACAAGAT TTCCACCGAA 
GACCTCATCA AGCTCATCAC CGAGTACAGG AGGGCGGAGA AGGCCAGTAA ACTCCAGACC 
ATCCCTGGAA ACCTTGAAAT AAATGTGGAC TGTGTTCCCA TGGAATGTCC CAATTGTGTG 
ACGTCCTCAT ATGTTGCCCT GAAGCCGTTT GAGGACTGCA GCCAACATGC TCCTACGGTG 
GAAATCGATG AGTTCCTGCA GGATTCGTCA AAGTTCGCTC AACCTTACAG AGTCTACAAG 
AACCACATCT ACATCTACCC TAAACACCTC AAGTATGACA GCCAGAAGAG CTTTGCTAAG 
GCTCGAAATA TTGCAGTTTG TGTGGAGTTT CGAAGCTCCG ATGAAGAACA TGCCAAGCCG 
CTTAAGTGCA TCTATGGAAA GCCCGGAGGC CCAGTTTTTA CCACGACTGC CTGTACCACA 
GTTCTGCACC ATTCACAGAA CCCAGACTTT TATGATGAGG TCAAGATTGA GTTGCCCACA 
CATCTTCATG AGAAACACCA TATACTCTTC TCCTTCTATC ATGTGACCTG TGACATCAAT 
GCCAAGACCA GCTCTAAGAA GAAAGAGGCT CTTGAATTGC CAGTGGGTTA TGCATGGCTG 
CCTCTTCTAA AGGATGGACG CATTTCCTCT CAGGACTTCA GCATTCCTGT CTCCTGCACT 
TTGCCTGCTG GCTATTTACA CATTAAAGAG CCTTCGTCCA CCAAGAATGG CTCTGATGTG 
AAGTGGGTCG ATGGAGGGAA GCAGATCTTC AAATTGTCCA CTTTGGTGCT TTCTACAGTG 
TATACTCAGG ATCCCCATCT AAACCGCTTC TTCCAGCAGT GCCAAAAACG AGAGGCAGAA 
ATCTCTCAAC CTCCTACCTC AAACTTCCTC AACTGCCTTA AAGGGCTCTT GAGCATGGAG 
AAGATTCATG CCATAATTCG CTTCCTTCCT GTTATCTTCA ACCAGCTCTT TAAAGTGCTT 
ACGCAAAACG ACTCTGATGA AGTCACCACC AGCACCACCA GGGTCCTGAT ACACATCTTA 
GCCAAGTGCC ACGAGGAGAA CCTGGACCAC TATTTGCACT CCTACATTAA GTTTGTCTTT 
AAGACTGAAG CGTGTGGCTT CAGAACCATT CATGAAGAGC TGGCCAAGGG AATGACCTCT 
GACCTGAAGA GCAATGAACA GTTAGTCATA CGCAACGTCC TGAAGTTCTC GTGGTTCTTT 
CTCGAGATCA TAGTGAAGTC AATGGCTCAG CATCTGCTGG ACTCAAACAA GCTCAAGCTG 
CCGCGACCGC AACGATTTCC TGGCACTTTC CAAAACCGTT TGGAGACGTT GATCATGACC 
ATGTCGGATC ACATTTACTG GAAGAACAAA GAGCTGGCGG AGGAGACGCG GAGTGCAAAT 
ATGGCCATTG CGTTTTTTGT GAAGCGGTGC TTCACCTTGA TGGACAGAGG TTTCACCTTT 
AAACTGATCA GCAACTACCT GAACATGATC ACAGCCACTG ACAATAAGGC GCTGTGTGAG 
CTGAAGTTTG AGTTCATAAG GGAAGTGTGT AACTATGAGC ACTTTGTCCC TTTGAGTCTT 
CCCATCCCGT CTGCACGAAT CACAGACAAT GCATCAACAG AAGCCCAAAG TACTAAGGTG 
GACTTCCCAG AGTACAGTTT GACAGAGGAT TTCTGCAGGA AGCATTTTCT GTCTGGATTG 
TTGTTGCGAG AGCTGGGACT CGCTTTGCAG GATGAGCAAG ATCTGAGACA CATCGCTCTG 
GCCTGCCTGA AGAACCTGAT GGCCAAACAC TCGCTGGACG CTCGATACGC CACAAAGGAG 
AAACAGGCTC GGATAGCTTC TCTTTACCTG CCGCTTTATG GCCTGATCCT GGACAACATG 
CCTCGGTTTT TCCTCAGAGA TCTGTTTCCA ATCTGCCTGA CAGCGAGTGA CCAGGGATCC 
AGAGATGATC TCAGTGTTGG AGTAGGTTTG GGTGGTCAGG TGACCCATGT TTTACGCCAT 
GGAAACTCAA TGGACGCGTC CTTCTCCAAG GAAGTGCTCA ACTCCATTAC AGCCTTTTCA 
TCATTGGCTG TATCGACCGC TAATCATGCC GACTCCAGGG GTTCTCTGAT CAGCATCGAG 
TCGAACCCCA GCAACAGTGA CCGCAACAGT GAGAAGATGG ATGCCTGTGA AAAGTTTGCC 
AGGCCTCAGT CTCTTATTGG CTTTGGATCC CGCTTTGATA AACTGGATCA AGCTGAAACC 
AGAAGTCTGC TCATGTGTTT CCTGCACATT ATGAAGACCA TCTCAGAGGA GGTGATGGTT 
TCATACTGGC ATCGAGCGAT TCACCAGGAG ATCAGTGACT TCTTCAATAT TTTGGAATTA 
TGCCTTCAGC ACTTTCGTTT CCTTGGAAAG CGGCACATTG CCAGGAAGCT AGCGGCCGCA 
GTTAAGCTGG CTCAGGCCAC ACAGAACAAT GGCACCCTGA AGGGCTCTAA TGTGTCCTAC 
CACTCTCCAG GGCTCCTACC CCAATGGATT CTGTCTGCAC AGGACGGTCA CAGACACGCT 
CGCTCTCAGA CCATGCCCAT CATCAGGGGG AAAAACGCCC TCACAAACCC CAAACTGCTG 
CATATGATGG AGACAGATGG AAATGTTGCT GAAGGAGAAA ACTTTAACCC TACAGACGTG 
GAGGCGAACC TTTCTACTGA AGTAGCACTG ACCGTGTTGG ATGTTCTGGA CCTGTTTGTT 
CGACATCATA AGAAACAGTT GCAGCACGAT GAAGGACAAA ACTCTTTGAT GAAGAAAGTG 
TTCGACACGT ACCTGCTGTT CTTCCAGATC AACCAGTCCA CCACCACCCT CAGACACGTG 
TTCACAGCCC TGCGCCTCTT CATGCAGAAG TTTCCGTCCA CGTTTTTCCA GGGTAAAGCG 
GATGTGTGTG GCAGCCTGTG TTACGAGATC CTGAAGTGCT GTAACCATCG CTCTAGCTCC 
ACACAGACTG AAGCCGCCGC TCTGCTTTAT TTCCTCATGA GGAGGAACTT TGAGTTCACG 
AAGGGCAAGT GCATCGTCCG CTCACACCTG CAGGTGATTA AAGCTGTGAG TCAGCTGATC 
GCGGATGCAG GCATTGGCGG ATCACGCTTC CAGCAGTCTC TGGCCATCAT CAATAACTTC 
GCCAATGGGG ACACGCCACT TAAAAACACC ACATTCCCAG CTGACGTAAA GGACCTGACC 
AAGAGGATAC GCACCGTCCT GATGGCCACG TCTCAAATGA AAGAGCATGA AAAAGACCCG 
GAAATGCTGG TGGACCTTCA GTACAGTCTG GCCAACTCCT ACGCCAGCAC ACCTGAGCTC 
AGACGCACCT GGCTGGAAAG CATGGCCAAA ATACACGTCA GAAATGGAGA TCTGTCTGAG 
GCAGCCATGT GCTACATCCA CATCTCAGCC CTCATAGCAG AGTCTCTGAG GAGAAGAGGT 
TACTGGAAGT CTGAAAAGGG TCGGATGTCA AGTGTGAGCA AAGAGGGAGC CTGTGTAAAT 
AACTGTAGCC CTTTACTAAC TCCCCAAGAC GGAGAAACAT CATTCAGTAT GGGTTGGGCG 
GCATTTACGA ATATCTCGCC TAACGTTAAA GAAGAGGGAG CAATGAAAGA AGACGCTGGC 
ACTCAGGATA CTCCATACAC TGAGGACACG CTGGTGGAGC AGCTGGAGAT GTGTGTTGAT 
TATCTCTGGA AGTCTGAGAG ATATGAACTT ATTGCCGAGA TCAACAAACC TGTTATTGTC 
GTCTTTGAGA AGAGAAGAGA TTTCAAGCGT CTATCAGAGC TGTACTATGA CATTCACCGC 
TCATACCTGA AGGTGACCGA AGTCGTGAAT TCTGAGAAAC GTCTGTTTGG TCGGTACTAT 
CGCGTGGCTT TCTACGGACA GGGGTTTTTT GAGGAAGAGG AGAGTAAGGA GTTTATCTAC 
AAGGAGCCCA AACTCACAGG ACTGTCCGAA ATTTCTCAAA GACTCCTCAA ACTCTACTCG 
GACAAGTTTG GAGCTGATAA CGTCAAAATG ATCCAGGACT CCAACAAGGT GAATCCGAAA 
GACTTGGACC CCAGATTTGC CTACATCCAG GTCACCTATG TAGTGCCTTA TTTTAATGAG 
AAGGAACAGC TGGAGAAGAA AACCGACTTC GAGCGCCATC ACAACATCGA CCGATTTGTG 
TTTGAGACGC CCTTCACGCT TTCTGGAAAA AAACACGGAG ACGTGGAGGA ACAGTGCAAA 
AGGAAAACCA TCCTGACCAC GAGCTCTAGT TTCCCTTTCT TGAAGAAGCG TATAGAGGTG 
GTGGAGCAAC AGAGTACCGA GATGAACCCT ATTGAGGTGG CGATAGACGA GATGAGCCGA 
AAAGTGTCTG AACTCAAACA GCTGTGCAAT ATGGAGGAGG TGGACATGAT CCGTCTGCAG 
CTCAAACTAC AGGGCAGCGT CAGCGTCAAG GTAAATGCAG GACCCATGGC CTACGCCCGA 
GCCTTCCTAG AGGAGAAAAA TGCTAAGAAA TACCCAGACA ACCAGGTCAA ACTGCTGAAA 
GAGATCTTCA GACAGTTTGC AGAAGCGTGT GGACAGGCTC TGAGTGTGAA CGAGCGGCTA 
ATAAAAGAGG ATCAGTTTGA GTATCAGAGC GAGATGAAAG CTCACTATAA AGACATGCTC 
ACGGAGCTCT CCGCCATTAT GAATGAGCAG ATACCGTACA CGAGACAACC AGGAACAGAG 
CGACACAGTG CCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOCK10 ( XM_001341012.7 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_001341048.5 Danio rerio dedicator of cytokinesis 10 (dock10), transcript variant X1, mRNA.

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CloneID	ODa20115
Clone ID Related Accession (Same CDS sequence)	XM_001341012.8 , XM_001341012.7
Accession Version	XM_001341012.7	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	dedicator of cytokinesis protein 10 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001341012.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTGTA CCACGAGTCT ACTACAGGCT GTGAATTTTA TAATGAGGAG AAACCATTCA 
CGCGTCTTTT CTGACCAGCA GAAACAACAT TTGCTGGAAA CTGCACCGCT GGAGGAGGGG 
ATGGTCATCA GTGAACCACA CAGCACTGTC GTCATGCAGG AGAAGCCCAA ACTGATCGAC 
CCTCTGGACT ATGAAGCTGT GATTTCTGAA CTTGAAGCAG AATTCAGAGA AGATCCTCTT 
CAAGATCTCC TGTTATTCCC AGACCTTGAC TTCACTGTGT CCACACTTCC TGTAGAAAGG 
AGAACAGTGA AGTCTACTGT CCCAGAGGGA GCTGATGAAC ATGCTGAATG TCTGCTGGTC 
AGACAGGCCT GCCAATACTT CAACTCCCAG CTGCACATTG TTCAGTATAA ATATGATGAG 
TATGGAGGGG ACTACCGCAT GCTGCCCAGG AAGCTGTGTA AAGCAGAAAA GTTGCCTTCC 
CACTCTTTTG AGATTGACAA TGAAGATGCG GACAAAGATG AGGACACAAC ATCTTTGTCC 
TCATCTAAAG GAGGGGGCGG CGGTGCAGGC AGTGGAGGCG GAGGAGGAAT TGGTGTTTTT 
AAGTCCGGCT GGCTCTATAA AGGCAACTTC AACAGCACCG TCAACAACAG CATCACAGTT 
CGGTCCTTCA AGCGCCGATA TTTTCAGCTG ACTCAGCTCT CTGACAACTC TTACATCATG 
AACTTCTATA AAGATGAGAA AATTTCCAAG GAACCGAAAG GCTGTATTTT TCTGGACTCC 
TGCACCGGGG TGGTTCAGAA TAATCGACTG AGGAAACATG CGTTTGAGCT GAAAATGAAC 
GAAGTGACAT ATTTTGTCCT GGCTGCCGAG AATGAGCAAG ACATGGACGA CTGGATCAAC 
ACTTTAAATC GCATCCTGCA GATCAGCCCT CCAGACGGCC CGGCTCAGGA CCGCAAGAGT 
ACGGACATGA GCGACTCTAA AAACGAGATC AGTGATATAC CACAAAATAA CGTCCCTGTT 
CATAAAAACG AAGAAACAAC TGAAAATGAG ATCCACCCTG AACTAGCCAA GTATATTTCT 
GAGACAGAAG AAAGCTCGAG AGCTGTCAGA GCAGAGGAGA GACTGAACCT GTTCTCCCTG 
GACCCTGATA TTCCTGTTTT AAGGAGTCCT AGGACAGAAA CCGGGGAGTC TTCTTCTGTC 
CATCCATTTG ATGAGAAGCT TGGGAAGAGG CTGATGATCT GCTGTCGCTC ACTGAACCTC 
ACACTGCAAG GCTGCGTTAA TGAAGCGGAC ACCGAACCCA CCACTAATAT CGAGCCTTTC 
TTTGTGTCAG TGTGTCTGCT GGACGTACGT GAGGGCAGGA AGGTTTCCGC TGACTTCCAT 
GTGGACGTAA ACCATGAGTT AGTGCGGCAG ATGCTTAATA GCTCTGGTAA TCCGGGAGAT 
CAAGGCTCGG ATGTTGGTAA TGGTGCACCG CTGGAAAATG GACTCACTTC CCCGGTAGAA 
AAGAAACCAG AAGACTGTCA ACTAGGCCCA GACATGGAAA AGTGGCTATG CTTTCCCAAA 
CAGGCAATCT TTTCCATTAC GAACCCGCAC ACGGATATTG TGATGGTGGC ACGAGTGGAG 
AAAGTTCTGA TGGGAAATAT TTCCAGTGGT GCAGAGCCCT ACATCAAAAA CACCGATTCC 
AGCAAGACTG TACACAAGAT GCTGAAATCC AATAAGCAGT TCTGTAGCAA ACTAGGCAAG 
TACCGAATGC CTTTCGCCTG GTCTGTAAGG TCAGTGTTTA AGGACAACCA GGGCACAGTG 
GACAGGGAGT CTCGCTTCTC TCCTCTTTTC AAACAGGAAA GCAACAAGAT TTCCACCGAA 
GACCTCATCA AGCTCATCAC CGAGTACAGG AGGGCGGAGA AGGCCAGTAA ACTCCAGACC 
ATCCCTGGAA ACCTTGAAAT AAATGTGGAC TGTGTTCCCA TGGAATGTCC CAATTGTGTG 
ACGTCCTCAT ATGTTGCCCT GAAGCCGTTT GAGGACTGCA GCCAACATGC TCCTACGGTG 
GAAATCGATG AGTTCCTGCA GGATTCGTCA AAGTTCGCTC AACCTTACAG AGTCTACAAG 
AACCACATCT ACATCTACCC TAAACACCTC AAGTATGACA GCCAGAAGAG CTTTGCTAAG 
GCTCGAAATA TTGCAGTTTG TGTGGAGTTT CGAAGCTCCG ATGAAGAACA TGCCAAGCCG 
CTTAAGTGCA TCTATGGAAA GCCCGGAGGC CCAGTTTTTA CCACGACTGC CTGTACCACA 
GTTCTGCACC ATTCACAGAA CCCAGACTTT TATGATGAGG TCAAGATTGA GTTGCCCACA 
CATCTTCATG AGAAACACCA TATACTCTTC TCCTTCTATC ATGTGACCTG TGACATCAAT 
GCCAAGACCA GCTCTAAGAA GAAAGAGGCT CTTGAATTGC CAGTGGGTTA TGCATGGCTG 
CCTCTTCTAA AGGATGGACG CATTTCCTCT CAGGACTTCA GCATTCCTGT CTCCTGCACT 
TTGCCTGCTG GCTATTTACA CATTAAAGAG CCTTCGTCCA CCAAGAATGG CTCTGATGTG 
AAGTGGGTCG ATGGAGGGAA GCAGATCTTC AAATTGTCCA CTTTGGTGCT TTCTACAGTG 
TATACTCAGG ATCCCCATCT AAACCGCTTC TTCCAGCAGT GCCAAAAACG AGAGGCAGAA 
ATCTCTCAAC CTCCTACCTC AAACTTCCTC AACTGCCTTA AAGGGCTCTT GAGCATGGAG 
AAGATTCATG CCATAATTCG CTTCCTTCCT GTTATCTTCA ACCAGCTCTT TAAAGTGCTT 
ACGCAAAACG ACTCTGATGA AGTCACCACC AGCACCACCA GGGTCCTGAT ACACATCTTA 
GCCAAGTGCC ACGAGGAGAA CCTGGACCAC TATTTGCACT CCTACATTAA GTTTGTCTTT 
AAGACTGAAG CGTGTGGCTT CAGAACCATT CATGAAGAGC TGGCCAAGGG AATGACCTCT 
GACCTGAAGA GCAATGAACA GTTAGTCATA CGCAACGTCC TGAAGTTCTC GTGGTTCTTT 
CTCGAGATCA TAGTGAAGTC AATGGCTCAG CATCTGCTGG ACTCAAACAA GCTCAAGCTG 
CCGCGACCGC AACGATTTCC TGGCACTTTC CAAAACCGTT TGGAGACGTT GATCATGACC 
ATGTCGGATC ACATTTACTG GAAGAACAAA GAGCTGGCGG AGGAGACGCG GAGTGCAAAT 
ATGGCCATTG CGTTTTTTGT GAAGCGGTGC TTCACCTTGA TGGACAGAGG TTTCACCTTT 
AAACTGATCA GCAACTACCT GAACATGATC ACAGCCACTG ACAATAAGGC GCTGTGTGAG 
CTGAAGTTTG AGTTCATAAG GGAAGTGTGT AACTATGAGC ACTTTGTCCC TTTGAGTCTT 
CCCATCCCGT CTGCACGAAT CACAGACAAT GCATCAACAG AAGCCCAAAG TACTAAGGTG 
GACTTCCCAG AGTACAGTTT GACAGAGGAT TTCTGCAGGA AGCATTTTCT GTCTGGATTG 
TTGTTGCGAG AGCTGGGACT CGCTTTGCAG GATGAGCAAG ATCTGAGACA CATCGCTCTG 
GCCTGCCTGA AGAACCTGAT GGCCAAACAC TCGCTGGACG CTCGATACGC CACAAAGGAG 
AAACAGGCTC GGATAGCTTC TCTTTACCTG CCGCTTTATG GCCTGATCCT GGACAACATG 
CCTCGGTTTT TCCTCAGAGA TCTGTTTCCA ATCTGCCTGA CAGCGAGTGA CCAGGGATCC 
AGAGATGATC TCAGTGTTGG AGTAGGTTTG GGTGGTCAGG TGACCCATGT TTTACGCCAT 
GGAAACTCAA TGGACGCGTC CTTCTCCAAG GAAGTGCTCA ACTCCATTAC AGCCTTTTCA 
TCATTGGCTG TATCGACCGC TAATCATGCC GACTCCAGGG GTTCTCTGAT CAGCATCGAG 
TCGAACCCCA GCAACAGTGA CCGCAACAGT GAGAAGATGG ATGCCTGTGA AAAGTTTGCC 
AGGCCTCAGT CTCTTATTGG CTTTGGATCC CGCTTTGATA AACTGGATCA AGCTGAAACC 
AGAAGTCTGC TCATGTGTTT CCTGCACATT ATGAAGACCA TCTCAGAGGA GGTGATGGTT 
TCATACTGGC ATCGAGCGAT TCACCAGGAG ATCAGTGACT TCTTCAATAT TTTGGAATTA 
TGCCTTCAGC ACTTTCGTTT CCTTGGAAAG CGGCACATTG CCAGGAAGCT AGCGGCCGCA 
GTTAAGCTGG CTCAGGCCAC ACAGAACAAT GGCACCCTGA AGGGCTCTAA TGTGTCCTAC 
CACTCTCCAG GGCTCCTACC CCAATGGATT CTGTCTGCAC AGGACGGTCA CAGACACGCT 
CGCTCTCAGA CCATGCCCAT CATCAGGGGG AAAAACGCCC TCACAAACCC CAAACTGCTG 
CATATGATGG AGACAGATGG AAATGTTGCT GAAGGAGAAA ACTTTAACCC TACAGACGTG 
GAGGCGAACC TTTCTACTGA AGTAGCACTG ACCGTGTTGG ATGTTCTGGA CCTGTTTGTT 
CGACATCATA AGAAACAGTT GCAGCACGAT GAAGGACAAA ACTCTTTGAT GAAGAAAGTG 
TTCGACACGT ACCTGCTGTT CTTCCAGATC AACCAGTCCA CCACCACCCT CAGACACGTG 
TTCACAGCCC TGCGCCTCTT CATGCAGAAG TTTCCGTCCA CGTTTTTCCA GGGTAAAGCG 
GATGTGTGTG GCAGCCTGTG TTACGAGATC CTGAAGTGCT GTAACCATCG CTCTAGCTCC 
ACACAGACTG AAGCCGCCGC TCTGCTTTAT TTCCTCATGA GGAGGAACTT TGAGTTCACG 
AAGGGCAAGT GCATCGTCCG CTCACACCTG CAGGTGATTA AAGCTGTGAG TCAGCTGATC 
GCGGATGCAG GCATTGGCGG ATCACGCTTC CAGCAGTCTC TGGCCATCAT CAATAACTTC 
GCCAATGGGG ACACGCCACT TAAAAACACC ACATTCCCAG CTGACGTAAA GGACCTGACC 
AAGAGGATAC GCACCGTCCT GATGGCCACG TCTCAAATGA AAGAGCATGA AAAAGACCCG 
GAAATGCTGG TGGACCTTCA GTACAGTCTG GCCAACTCCT ACGCCAGCAC ACCTGAGCTC 
AGACGCACCT GGCTGGAAAG CATGGCCAAA ATACACGTCA GAAATGGAGA TCTGTCTGAG 
GCAGCCATGT GCTACATCCA CATCTCAGCC CTCATAGCAG AGTCTCTGAG GAGAAGAGGT 
TACTGGAAGT CTGAAAAGGG TCGGATGTCA AGTGTGAGCA AAGAGGGAGC CTGTGTAAAT 
AACTGTAGCC CTTTACTAAC TCCCCAAGAC GGAGAAACAT CATTCAGTAT GGGTTGGGCG 
GCATTTACGA ATATCTCGCC TAACGTTAAA GAAGAGGGAG CAATGAAAGA AGACGCTGGC 
ACTCAGGATA CTCCATACAC TGAGGACACG CTGGTGGAGC AGCTGGAGAT GTGTGTTGAT 
TATCTCTGGA AGTCTGAGAG ATATGAACTT ATTGCCGAGA TCAACAAACC TGTTATTGTC 
GTCTTTGAGA AGAGAAGAGA TTTCAAGCGT CTATCAGAGC TGTACTATGA CATTCACCGC 
TCATACCTGA AGGTGACCGA AGTCGTGAAT TCTGAGAAAC GTCTGTTTGG TCGGTACTAT 
CGCGTGGCTT TCTACGGACA GGGGTTTTTT GAGGAAGAGG AGAGTAAGGA GTTTATCTAC 
AAGGAGCCCA AACTCACAGG ACTGTCCGAA ATTTCTCAAA GACTCCTCAA ACTCTACTCG 
GACAAGTTTG GAGCTGATAA CGTCAAAATG ATCCAGGACT CCAACAAGGT GAATCCGAAA 
GACTTGGACC CCAGATTTGC CTACATCCAG GTCACCTATG TAGTGCCTTA TTTTAATGAG 
AAGGAACAGC TGGAGAAGAA AACCGACTTC GAGCGCCATC ACAACATCGA CCGATTTGTG 
TTTGAGACGC CCTTCACGCT TTCTGGAAAA AAACACGGAG ACGTGGAGGA ACAGTGCAAA 
AGGAAAACCA TCCTGACCAC GAGCTCTAGT TTCCCTTTCT TGAAGAAGCG TATAGAGGTG 
GTGGAGCAAC AGAGTACCGA GATGAACCCT ATTGAGGTGG CGATAGACGA GATGAGCCGA 
AAAGTGTCTG AACTCAAACA GCTGTGCAAT ATGGAGGAGG TGGACATGAT CCGTCTGCAG 
CTCAAACTAC AGGGCAGCGT CAGCGTCAAG GTAAATGCAG GACCCATGGC CTACGCCCGA 
GCCTTCCTAG AGGAGAAAAA TGCTAAGAAA TACCCAGACA ACCAGGTCAA ACTGCTGAAA 
GAGATCTTCA GACAGTTTGC AGAAGCGTGT GGACAGGCTC TGAGTGTGAA CGAGCGGCTA 
ATAAAAGAGG ATCAGTTTGA GTATCAGAGC GAGATGAAAG CTCACTATAA AGACATGCTC 
ACGGAGCTCT CCGCCATTAT GAATGAGCAG ATACCGTACA CGAGACAACC AGGAACAGAG 
CGACACAGTG CCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001341048.5
CDS	176..6733
Translation	MGCTTSLLQAVNFIMRRNHSRVFSDQQKQHLLETAPLEEGMVISEPHSTVVMQEKPKLIDPLDYEAVISELEAEFREDPLQDLLLFPDLDFTVSTLPVERRTVKSTVPEGADEHAECLLVRQACQYFNSQLHIVQYKYDEYGGDYRMLPRKLCKAEKLPSHSFEIDNEDADKDEDTTSLSSSKGGGGGAGSGGGGGIGVFKSGWLYKGNFNSTVNNSITVRSFKRRYFQLTQLSDNSYIMNFYKDEKISKEPKGCIFLDSCTGVVQNNRLRKHAFELKMNEVTYFVLAAENEQDMDDWINTLNRILQISPPDGPAQDRKSTDMSDSKNEISDIPQNNVPVHKNEETTENEIHPELAKYISETEESSRAVRAEERLNLFSLDPDIPVLRSPRTETGESSSVHPFDEKLGKRLMICCRSLNLTLQGCVNEADTEPTTNIEPFFVSVCLLDVREGRKVSADFHVDVNHELVRQMLNSSGNPGDQGSDVGNGAPLENGLTSPVEKKPEDCQLGPDMEKWLCFPKQAIFSITNPHTDIVMVARVEKVLMGNISSGAEPYIKNTDSSKTVHKMLKSNKQFCSKLGKYRMPFAWSVRSVFKDNQGTVDRESRFSPLFKQESNKISTEDLIKLITEYRRAEKASKLQTIPGNLEINVDCVPMECPNCVTSSYVALKPFEDCSQHAPTVEIDEFLQDSSKFAQPYRVYKNHIYIYPKHLKYDSQKSFAKARNIAVCVEFRSSDEEHAKPLKCIYGKPGGPVFTTTACTTVLHHSQNPDFYDEVKIELPTHLHEKHHILFSFYHVTCDINAKTSSKKKEALELPVGYAWLPLLKDGRISSQDFSIPVSCTLPAGYLHIKEPSSTKNGSDVKWVDGGKQIFKLSTLVLSTVYTQDPHLNRFFQQCQKREAEISQPPTSNFLNCLKGLLSMEKIHAIIRFLPVIFNQLFKVLTQNDSDEVTTSTTRVLIHILAKCHEENLDHYLHSYIKFVFKTEACGFRTIHEELAKGMTSDLKSNEQLVIRNVLKFSWFFLEIIVKSMAQHLLDSNKLKLPRPQRFPGTFQNRLETLIMTMSDHIYWKNKELAEETRSANMAIAFFVKRCFTLMDRGFTFKLISNYLNMITATDNKALCELKFEFIREVCNYEHFVPLSLPIPSARITDNASTEAQSTKVDFPEYSLTEDFCRKHFLSGLLLRELGLALQDEQDLRHIALACLKNLMAKHSLDARYATKEKQARIASLYLPLYGLILDNMPRFFLRDLFPICLTASDQGSRDDLSVGVGLGGQVTHVLRHGNSMDASFSKEVLNSITAFSSLAVSTANHADSRGSLISIESNPSNSDRNSEKMDACEKFARPQSLIGFGSRFDKLDQAETRSLLMCFLHIMKTISEEVMVSYWHRAIHQEISDFFNILELCLQHFRFLGKRHIARKLAAAVKLAQATQNNGTLKGSNVSYHSPGLLPQWILSAQDGHRHARSQTMPIIRGKNALTNPKLLHMMETDGNVAEGENFNPTDVEANLSTEVALTVLDVLDLFVRHHKKQLQHDEGQNSLMKKVFDTYLLFFQINQSTTTLRHVFTALRLFMQKFPSTFFQGKADVCGSLCYEILKCCNHRSSSTQTEAAALLYFLMRRNFEFTKGKCIVRSHLQVIKAVSQLIADAGIGGSRFQQSLAIINNFANGDTPLKNTTFPADVKDLTKRIRTVLMATSQMKEHEKDPEMLVDLQYSLANSYASTPELRRTWLESMAKIHVRNGDLSEAAMCYIHISALIAESLRRRGYWKSEKGRMSSVSKEGACVNNCSPLLTPQDGETSFSMGWAAFTNISPNVKEEGAMKEDAGTQDTPYTEDTLVEQLEMCVDYLWKSERYELIAEINKPVIVVFEKRRDFKRLSELYYDIHRSYLKVTEVVNSEKRLFGRYYRVAFYGQGFFEEEESKEFIYKEPKLTGLSEISQRLLKLYSDKFGADNVKMIQDSNKVNPKDLDPRFAYIQVTYVVPYFNEKEQLEKKTDFERHHNIDRFVFETPFTLSGKKHGDVEEQCKRKTILTTSSSFPFLKKRIEVVEQQSTEMNPIEVAIDEMSRKVSELKQLCNMEEVDMIRLQLKLQGSVSVKVNAGPMAYARAFLEEKNAKKYPDNQVKLLKEIFRQFAEACGQALSVNERLIKEDQFEYQSEMKAHYKDMLTELSAIMNEQIPYTRQPGTERHSAI

Target ORF information:

RefSeq Version	XM_001341012.7
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio dedicator of cytokinesis 10 (dock10), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001341012.7

ORF Insert Sequence:

ATGGGCTGTA CCACGAGTCT ACTACAGGCT GTGAATTTTA TAATGAGGAG AAACCATTCA 
CGCGTCTTTT CTGACCAGCA GAAACAACAT TTGCTGGAAA CTGCACCGCT GGAGGAGGGG 
ATGGTCATCA GTGAACCACA CAGCACTGTC GTCATGCAGG AGAAGCCCAA ACTGATCGAC 
CCTCTGGACT ATGAAGCTGT GATTTCTGAA CTTGAAGCAG AATTCAGAGA AGATCCTCTT 
CAAGATCTCC TGTTATTCCC AGACCTTGAC TTCACTGTGT CCACACTTCC TGTAGAAAGG 
AGAACAGTGA AGTCTACTGT CCCAGAGGGA GCTGATGAAC ATGCTGAATG TCTGCTGGTC 
AGACAGGCCT GCCAATACTT CAACTCCCAG CTGCACATTG TTCAGTATAA ATATGATGAG 
TATGGAGGGG ACTACCGCAT GCTGCCCAGG AAGCTGTGTA AAGCAGAAAA GTTGCCTTCC 
CACTCTTTTG AGATTGACAA TGAAGATGCG GACAAAGATG AGGACACAAC ATCTTTGTCC 
TCATCTAAAG GAGGGGGCGG CGGTGCAGGC AGTGGAGGCG GAGGAGGAAT TGGTGTTTTT 
AAGTCCGGCT GGCTCTATAA AGGCAACTTC AACAGCACCG TCAACAACAG CATCACAGTT 
CGGTCCTTCA AGCGCCGATA TTTTCAGCTG ACTCAGCTCT CTGACAACTC TTACATCATG 
AACTTCTATA AAGATGAGAA AATTTCCAAG GAACCGAAAG GCTGTATTTT TCTGGACTCC 
TGCACCGGGG TGGTTCAGAA TAATCGACTG AGGAAACATG CGTTTGAGCT GAAAATGAAC 
GAAGTGACAT ATTTTGTCCT GGCTGCCGAG AATGAGCAAG ACATGGACGA CTGGATCAAC 
ACTTTAAATC GCATCCTGCA GATCAGCCCT CCAGACGGCC CGGCTCAGGA CCGCAAGAGT 
ACGGACATGA GCGACTCTAA AAACGAGATC AGTGATATAC CACAAAATAA CGTCCCTGTT 
CATAAAAACG AAGAAACAAC TGAAAATGAG ATCCACCCTG AACTAGCCAA GTATATTTCT 
GAGACAGAAG AAAGCTCGAG AGCTGTCAGA GCAGAGGAGA GACTGAACCT GTTCTCCCTG 
GACCCTGATA TTCCTGTTTT AAGGAGTCCT AGGACAGAAA CCGGGGAGTC TTCTTCTGTC 
CATCCATTTG ATGAGAAGCT TGGGAAGAGG CTGATGATCT GCTGTCGCTC ACTGAACCTC 
ACACTGCAAG GCTGCGTTAA TGAAGCGGAC ACCGAACCCA CCACTAATAT CGAGCCTTTC 
TTTGTGTCAG TGTGTCTGCT GGACGTACGT GAGGGCAGGA AGGTTTCCGC TGACTTCCAT 
GTGGACGTAA ACCATGAGTT AGTGCGGCAG ATGCTTAATA GCTCTGGTAA TCCGGGAGAT 
CAAGGCTCGG ATGTTGGTAA TGGTGCACCG CTGGAAAATG GACTCACTTC CCCGGTAGAA 
AAGAAACCAG AAGACTGTCA ACTAGGCCCA GACATGGAAA AGTGGCTATG CTTTCCCAAA 
CAGGCAATCT TTTCCATTAC GAACCCGCAC ACGGATATTG TGATGGTGGC ACGAGTGGAG 
AAAGTTCTGA TGGGAAATAT TTCCAGTGGT GCAGAGCCCT ACATCAAAAA CACCGATTCC 
AGCAAGACTG TACACAAGAT GCTGAAATCC AATAAGCAGT TCTGTAGCAA ACTAGGCAAG 
TACCGAATGC CTTTCGCCTG GTCTGTAAGG TCAGTGTTTA AGGACAACCA GGGCACAGTG 
GACAGGGAGT CTCGCTTCTC TCCTCTTTTC AAACAGGAAA GCAACAAGAT TTCCACCGAA 
GACCTCATCA AGCTCATCAC CGAGTACAGG AGGGCGGAGA AGGCCAGTAA ACTCCAGACC 
ATCCCTGGAA ACCTTGAAAT AAATGTGGAC TGTGTTCCCA TGGAATGTCC CAATTGTGTG 
ACGTCCTCAT ATGTTGCCCT GAAGCCGTTT GAGGACTGCA GCCAACATGC TCCTACGGTG 
GAAATCGATG AGTTCCTGCA GGATTCGTCA AAGTTCGCTC AACCTTACAG AGTCTACAAG 
AACCACATCT ACATCTACCC TAAACACCTC AAGTATGACA GCCAGAAGAG CTTTGCTAAG 
GCTCGAAATA TTGCAGTTTG TGTGGAGTTT CGAAGCTCCG ATGAAGAACA TGCCAAGCCG 
CTTAAGTGCA TCTATGGAAA GCCCGGAGGC CCAGTTTTTA CCACGACTGC CTGTACCACA 
GTTCTGCACC ATTCACAGAA CCCAGACTTT TATGATGAGG TCAAGATTGA GTTGCCCACA 
CATCTTCATG AGAAACACCA TATACTCTTC TCCTTCTATC ATGTGACCTG TGACATCAAT 
GCCAAGACCA GCTCTAAGAA GAAAGAGGCT CTTGAATTGC CAGTGGGTTA TGCATGGCTG 
CCTCTTCTAA AGGATGGACG CATTTCCTCT CAGGACTTCA GCATTCCTGT CTCCTGCACT 
TTGCCTGCTG GCTATTTACA CATTAAAGAG CCTTCGTCCA CCAAGAATGG CTCTGATGTG 
AAGTGGGTCG ATGGAGGGAA GCAGATCTTC AAATTGTCCA CTTTGGTGCT TTCTACAGTG 
TATACTCAGG ATCCCCATCT AAACCGCTTC TTCCAGCAGT GCCAAAAACG AGAGGCAGAA 
ATCTCTCAAC CTCCTACCTC AAACTTCCTC AACTGCCTTA AAGGGCTCTT GAGCATGGAG 
AAGATTCATG CCATAATTCG CTTCCTTCCT GTTATCTTCA ACCAGCTCTT TAAAGTGCTT 
ACGCAAAACG ACTCTGATGA AGTCACCACC AGCACCACCA GGGTCCTGAT ACACATCTTA 
GCCAAGTGCC ACGAGGAGAA CCTGGACCAC TATTTGCACT CCTACATTAA GTTTGTCTTT 
AAGACTGAAG CGTGTGGCTT CAGAACCATT CATGAAGAGC TGGCCAAGGG AATGACCTCT 
GACCTGAAGA GCAATGAACA GTTAGTCATA CGCAACGTCC TGAAGTTCTC GTGGTTCTTT 
CTCGAGATCA TAGTGAAGTC AATGGCTCAG CATCTGCTGG ACTCAAACAA GCTCAAGCTG 
CCGCGACCGC AACGATTTCC TGGCACTTTC CAAAACCGTT TGGAGACGTT GATCATGACC 
ATGTCGGATC ACATTTACTG GAAGAACAAA GAGCTGGCGG AGGAGACGCG GAGTGCAAAT 
ATGGCCATTG CGTTTTTTGT GAAGCGGTGC TTCACCTTGA TGGACAGAGG TTTCACCTTT 
AAACTGATCA GCAACTACCT GAACATGATC ACAGCCACTG ACAATAAGGC GCTGTGTGAG 
CTGAAGTTTG AGTTCATAAG GGAAGTGTGT AACTATGAGC ACTTTGTCCC TTTGAGTCTT 
CCCATCCCGT CTGCACGAAT CACAGACAAT GCATCAACAG AAGCCCAAAG TACTAAGGTG 
GACTTCCCAG AGTACAGTTT GACAGAGGAT TTCTGCAGGA AGCATTTTCT GTCTGGATTG 
TTGTTGCGAG AGCTGGGACT CGCTTTGCAG GATGAGCAAG ATCTGAGACA CATCGCTCTG 
GCCTGCCTGA AGAACCTGAT GGCCAAACAC TCGCTGGACG CTCGATACGC CACAAAGGAG 
AAACAGGCTC GGATAGCTTC TCTTTACCTG CCGCTTTATG GCCTGATCCT GGACAACATG 
CCTCGGTTTT TCCTCAGAGA TCTGTTTCCA ATCTGCCTGA CAGCGAGTGA CCAGGGATCC 
AGAGATGATC TCAGTGTTGG AGTAGGTTTG GGTGGTCAGG TGACCCATGT TTTACGCCAT 
GGAAACTCAA TGGACGCGTC CTTCTCCAAG GAAGTGCTCA ACTCCATTAC AGCCTTTTCA 
TCATTGGCTG TATCGACCGC TAATCATGCC GACTCCAGGG GTTCTCTGAT CAGCATCGAG 
TCGAACCCCA GCAACAGTGA CCGCAACAGT GAGAAGATGG ATGCCTGTGA AAAGTTTGCC 
AGGCCTCAGT CTCTTATTGG CTTTGGATCC CGCTTTGATA AACTGGATCA AGCTGAAACC 
AGAAGTCTGC TCATGTGTTT CCTGCACATT ATGAAGACCA TCTCAGAGGA GGTGATGGTT 
TCATACTGGC ATCGAGCGAT TCACCAGGAG ATCAGTGACT TCTTCAATAT TTTGGAATTA 
TGCCTTCAGC ACTTTCGTTT CCTTGGAAAG CGGCACATTG CCAGGAAGCT AGCGGCCGCA 
GTTAAGCTGG CTCAGGCCAC ACAGAACAAT GGCACCCTGA AGGGCTCTAA TGTGTCCTAC 
CACTCTCCAG GGCTCCTACC CCAATGGATT CTGTCTGCAC AGGACGGTCA CAGACACGCT 
CGCTCTCAGA CCATGCCCAT CATCAGGGGG AAAAACGCCC TCACAAACCC CAAACTGCTG 
CATATGATGG AGACAGATGG AAATGTTGCT GAAGGAGAAA ACTTTAACCC TACAGACGTG 
GAGGCGAACC TTTCTACTGA AGTAGCACTG ACCGTGTTGG ATGTTCTGGA CCTGTTTGTT 
CGACATCATA AGAAACAGTT GCAGCACGAT GAAGGACAAA ACTCTTTGAT GAAGAAAGTG 
TTCGACACGT ACCTGCTGTT CTTCCAGATC AACCAGTCCA CCACCACCCT CAGACACGTG 
TTCACAGCCC TGCGCCTCTT CATGCAGAAG TTTCCGTCCA CGTTTTTCCA GGGTAAAGCG 
GATGTGTGTG GCAGCCTGTG TTACGAGATC CTGAAGTGCT GTAACCATCG CTCTAGCTCC 
ACACAGACTG AAGCCGCCGC TCTGCTTTAT TTCCTCATGA GGAGGAACTT TGAGTTCACG 
AAGGGCAAGT GCATCGTCCG CTCACACCTG CAGGTGATTA AAGCTGTGAG TCAGCTGATC 
GCGGATGCAG GCATTGGCGG ATCACGCTTC CAGCAGTCTC TGGCCATCAT CAATAACTTC 
GCCAATGGGG ACACGCCACT TAAAAACACC ACATTCCCAG CTGACGTAAA GGACCTGACC 
AAGAGGATAC GCACCGTCCT GATGGCCACG TCTCAAATGA AAGAGCATGA AAAAGACCCG 
GAAATGCTGG TGGACCTTCA GTACAGTCTG GCCAACTCCT ACGCCAGCAC ACCTGAGCTC 
AGACGCACCT GGCTGGAAAG CATGGCCAAA ATACACGTCA GAAATGGAGA TCTGTCTGAG 
GCAGCCATGT GCTACATCCA CATCTCAGCC CTCATAGCAG AGTCTCTGAG GAGAAGAGGT 
TACTGGAAGT CTGAAAAGGG TCGGATGTCA AGTGTGAGCA AAGAGGGAGC CTGTGTAAAT 
AACTGTAGCC CTTTACTAAC TCCCCAAGAC GGAGAAACAT CATTCAGTAT GGGTTGGGCG 
GCATTTACGA ATATCTCGCC TAACGTTAAA GAAGAGGGAG CAATGAAAGA AGACGCTGGC 
ACTCAGGATA CTCCATACAC TGAGGACACG CTGGTGGAGC AGCTGGAGAT GTGTGTTGAT 
TATCTCTGGA AGTCTGAGAG ATATGAACTT ATTGCCGAGA TCAACAAACC TGTTATTGTC 
GTCTTTGAGA AGAGAAGAGA TTTCAAGCGT CTATCAGAGC TGTACTATGA CATTCACCGC 
TCATACCTGA AGGTGACCGA AGTCGTGAAT TCTGAGAAAC GTCTGTTTGG TCGGTACTAT 
CGCGTGGCTT TCTACGGACA GGGGTTTTTT GAGGAAGAGG AGAGTAAGGA GTTTATCTAC 
AAGGAGCCCA AACTCACAGG ACTGTCCGAA ATTTCTCAAA GACTCCTCAA ACTCTACTCG 
GACAAGTTTG GAGCTGATAA CGTCAAAATG ATCCAGGACT CCAACAAGGT GAATCCGAAA 
GACTTGGACC CCAGATTTGC CTACATCCAG GTCACCTATG TAGTGCCTTA TTTTAATGAG 
AAGGAACAGC TGGAGAAGAA AACCGACTTC GAGCGCCATC ACAACATCGA CCGATTTGTG 
TTTGAGACGC CCTTCACGCT TTCTGGAAAA AAACACGGAG ACGTGGAGGA ACAGTGCAAA 
AGGAAAACCA TCCTGACCAC GAGCTCTAGT TTCCCTTTCT TGAAGAAGCG TATAGAGGTG 
GTGGAGCAAC AGAGTACCGA GATGAACCCT ATTGAGGTGG CGATAGACGA GATGAGCCGA 
AAAGTGTCTG AACTCAAACA GCTGTGCAAT ATGGAGGAGG TGGACATGAT CCGTCTGCAG 
CTCAAACTAC AGGGCAGCGT CAGCGTCAAG GTAAATGCAG GACCCATGGC CTACGCCCGA 
GCCTTCCTAG AGGAGAAAAA TGCTAAGAAA TACCCAGACA ACCAGGTCAA ACTGCTGAAA 
GAGATCTTCA GACAGTTTGC AGAAGCGTGT GGACAGGCTC TGAGTGTGAA CGAGCGGCTA 
ATAAAAGAGG ATCAGTTTGA GTATCAGAGC GAGATGAAAG CTCACTATAA AGACATGCTC 
ACGGAGCTCT CCGCCATTAT GAATGAGCAG ATACCGTACA CGAGACAACC AGGAACAGAG 
CGACACAGTG CCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

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オンライン注文

DOCK10 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報