scube3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	scube3
Entrez Gene ID	100000687
Full Name	signal peptide, CUB domain, EGF-like 3
General protein information	Preferred Names signal peptide, CUB domain, EGF-like 3 Names signal peptide, CUB and EGF-like domain-containing protein 3
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021469709.1	XP_021325384.1	signal peptide, CUB and EGF-like domain-containing protein 3 isoform X1
NM_001260501.1	NP_001247430.1	signal peptide, CUB and EGF-like domain-containing protein 3 precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SCUBE3	XP_005223362.1
Pan troglodytes (chimpanzee)	SCUBE3	XP_003311327.1
Mus musculus (house mouse)	Scube3	NP_001004366.1
Gallus gallus (chicken)	SCUBE3	XP_004935091.1
Homo sapiens (human)	SCUBE3	NP_689966.2
Canis lupus familiaris (dog)	SCUBE3	XP_005627308.1
Rattus norvegicus (Norway rat)	Scube3	NP_001258291.1
Danio rerio (zebrafish)	scube3	NP_001247430.1

Gene Ontology
Bibliography

Related articles in PubMed

Whole exome sequencing coupled with unbiased functional analysis reveals new Hirschsprung disease genes.
Gui H, Schriemer D, Cheng WW, Chauhan RK, Anti?olo G, Berrios C, Bleda M, Brooks AS, Brouwer RW, Burns AJ, Cherny SS, Dopazo J, Eggen BJ, Griseri P, Jalloh B, Le TL, Lui VC, Luz?n-Toro B, Matera I, Ngan ES, Pelet A, Ruiz-Ferrer M, Sham PC, Shepherd IT, So MT, Sribudiani Y, Tang CS, van den Hout MC, van der Linde HC, van Ham TJ, van IJcken WF, Verheij JB, Amiel J, Borrego S, Ceccherini I, Chakravarti A, Lyonnet S, Tam PK, Garcia-Barcel? MM, Hofstra RM
Genome biology18(1)48(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Molecular cloning, tissue expression pattern, and copy number variation of porcine SCUBE3.
Liu X, Wang LG, Zhang LC, Yan H, Zhao KB, Liang J, Li N, Pu L, Zhang T, Wang LX
Genetics and molecular research : GMR15(1)(2016 Feb)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Scube3 may be a critical upstream regulator of fast fiber myogenesis by modulating fgf8 signaling during zebrafish embryogenesis
Title: SCUBE3 (signal peptide-CUB-EGF domain-containing protein 3) modulates fibroblast growth factor signaling during fast muscle development.

Data show that knocking down the function of all three scube genes scube1, scube2 and scube3 simultaneously phenocopies a complete loss of Hedgehog (HH) signal transduction in the embryo.
Title: Scube activity is necessary for Hedgehog signal transduction in vivo.

The following scube3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the scube3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa04909	NM_001260501.1 Latest version!	Danio rerio signal peptide, CUB domain, EGF-like 3 (scube3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa151413	XM_021469709.1 Latest version!	Danio rerio signal peptide, CUB domain, EGF-like 3 (scube3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

scube3 ( NM_001260501.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001247430.1 Danio rerio signal peptide, CUB domain, EGF-like 3 (scube3), mRNA.

Price & Availability↑

CloneID	ODa04909
Clone ID Related Accession (Same CDS sequence)	NM_001260501.1
Accession Version	NM_001260501.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2988bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-31
Organism	Danio rerio(zebrafish)
Product	signal peptide, CUB and EGF-like domain-containing protein 3 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from JF900368.1. On May 10, 2012 this sequence version replaced XM_001340802.4. ##Evidence-Data-START## Transcript exon combination :: JF900368.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCGCGCTG GTTTTGGATT ATTCAGCGTC GCTCTGGTCG CCTTTGTCCA GGGTCCAGCC 
GGTGTTTTTG CCAGCAGAAA CCCGCAAGAT GTGGACGAGT GCGCCGAGGC CCTGGACAGC 
TGCAGTATAG ATGCCATCTG TCAGAACACC CTCAAGTCAT ACAAATGCAT CTGCAAATCA 
GGATACAAAG GCGACGGGAA ACACTGTGAA GACATCGATG AGTGTGCCAG CGAATATAAT 
GGAGGATGTG TTCACGAGTG CATCAACATC CCTGGAAACT ACAGATGCAC GTGTTATGAC 
GGCTTCCGTC TGGCCCACGA CGGACACAAC TGCTTGGATG TGGATGAATG TGCTGAAGGA 
AACGGCGGCT GTCAGCAGAT CTGTGTTAAC ATGATGGGCA GTTATGAATG TCGCTGCAGA 
GACGGCTTTC TGCTGTCCGA CAACCAGCAC ACCTGCATCC AGAGGCCCAA AGTACGTTTC 
AATGGAACTA AGGATGTGGC CACCTGCATG GATAAAAATC ACGGCTGTGC CCATATTTGC 
CGTGAGACCC CTAAAGGAAC CATCGCCTGT GAATGCAGAC CAGGATTTCA GCTCACCAAG 
AACAATCGAG ACTGTAAATT GACCTGTAAC TATGGTAATG GCGGCTGTCA GCACATCTGT 
GAGGAAACGG ATCATGGGCC CAAATGCTCG TGTCACATGA AGTTTGCTCT TCACTCAGAT 
GGAAAGACCT GTGTAGAGAC GTGTGCAGTG AATAACGGTG GTTGTGACAG CACGTGTCAT 
GATGCTGTGA CTGGTGTTCG CTGCAGCTGC CCGGTGGGAT TCACTTTACA GCCCGACCGC 
AAGACCTGCA AAGACATTGA TGAATGTCGC ATGAACAACG GTGGCTGTGA TCACGTTTGC 
AGAAACACCG TCGGCAGCTT CGAGTGCAGC TGCAAGAAAG GATACAAACT CCTGACCAAT 
GAGAGGACGT GCCAAGATAT AGACGAGTGT TCGTTTGACC GGGCCTGTGA CCACACATGC 
ATAAATTCAC CCGGGAGTTT CCAATGCTAC TGTCATAAAG GCTACGTCCT CTACGGCGTG 
GCTCACTGCG GAGATATCGA CGAGTGCAGC ATTAATCAGG GTGGCTGTAA ATCGGGCTGT 
CTTAACACGC TGGGCAGTTA TGAATGTACC TGTCCTCCTG GATACAAACT GCACTGGAAC 
AAGAAGGACT GTGTGGAGCT CATAAAATGT GCCTCCAATT TGGTGGCCCC CAAAGCCACC 
CTGGCCTGCA GTAAAACGGG CAAGAAAGAG AGCTGTTCCC TCACGTGTGC ATCCAAAGCA 
CATTATCTGT CAGAATCAGA CAGCAGTTAT TCTGTCAGCT GTGGAATATC AATCCTGCGA 
GGAAAATCTC AGAGCAGAGT CAACACGAGC AGCATCCAGA GCTGCATAGA GACTCAGGTT 
CATCCAGTGA AGCAAACGGC GTCTTTTCGG ATCAAGAACG CCAAATGTCA CCTGCACCCG 
AAGCATAAGG GGAGAGCAGA GGACAACGGC AGACAGATCG GCCCAGGCGG TCAGCCGTGC 
AACAACTGCC TGGTCACGTT TGTTAACCTG AAGTGTGACT CCTCTAAGAA GACCAAAGGC 
GGTCGTCGAG CTCGAAACCC GACAAATAAA GAGGTGACGC GCATTACTCT GGAGCTGGAG 
GCCGAGGTCA AACCGGGAGA CGCAACCGGG AACTGCAACA TGATGTGTCA ACGGCAGCAC 
ATGGAGCAGC AGATGAAGAC GTACATTAAA ACCCTGAAGA AGTCCATTAA CCAGGAGCGT 
TTCCTGCTGC GTGTCGCTGG ACTGGAGTAT GAGGTGGCAC AGAAGCTCAC ACAGACAGCG 
TTGAAGCAGG AGAACTGCGG ACCGGGCCGA GAAAAGGACA GCGGACGTTG TGTCAGATGT 
TTGCCTGGCT CATATTACCA TGGGGAGCAG GAGCGATGCA TCCAGTGCCC ACCGGGAACC 
TTCCAGGAGC GGGAAGGTCA GCTTGCCTGC GATCTGTGTC CTGGAGGAGA CCGGCATGGC 
CCACAAGGAG CCCGCAACAT CACGTCATGT GCAGGTCAGT GTCCACCAGG GCATTTCTCC 
AGCGACGGGT TCAAGCCCTG TCAGCCGTGT CCATTGGGAA AATACCAGCC AGATCCAGGA 
CGGACCCTCT GTTTCTCCTG TGGAGGTGGA CTCAGTACTA AAAGAGAGGG CGCCAGTTCA 
TTCAATGACT GCGAGGTCAA AGTGTTGTGT TCTCCAGGTC ATTATTATAA CACATCAGTT 
CATCGGTGTA TCCGCTGTCC TCATGGAACC TACCAGGCTG AATTCAGACA GAACTACTGC 
ATCTCCTGTC CTGGAAACAC CACCACAGAC TTTGACGGAG CCATCAGTGT GTCTCAGTGC 
AAGAATCGCG AATGCGGGGG TGAAATCGGC GAGTTCATTG GATACATCGA GTCACCCAAC 
TATCCCGGAA ACTACCCAGC CAATGTGGAG TGTGTGTGGA CCATCAACCC TCCACACAAA 
CGCAAGATTC TGATTGTGGT TCCTGAGATC TTCCTCCCGT CTGAGGATGA GTGTGGAGAT 
GTGCTGGTCA TGAGGAAGAA CTGGTCTGCA ACCTCTATTA CCACCTATGA GACGTGTCAG 
ACATACGAGA GACCGATTGC ATTTACAGCC CGATCGAGGC GCCTCTGGAT CAACTTCAAA 
TCCAATGATG TGAACAGCGC TCGTGGTTTC CAGATTCCAT ACGTCACTTA TGATGAAGAC 
TATGAGCAGC TGGTTGAAGA TATCGTAAGA GACGGACGGC TTTATGCCTC TGAAAACCAT 
CAAGAAATAC TCAAGGATAA AAAACTGATT AAAACACTGT TCGACGTTCT GGCTCACCCT 
CACAATTACT ACAAGTACTC GACTCGAGAC TCCACGGAGA TGCTCCCGCG CTCCTTCATT 
CGCCTCATCC ACAGCAAAGT CTCGGCCTTC CTACGTCCGT ACAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247430.1
CDS	208..3195
Translation	MRAGFGLFSVALVAFVQGPAGVFASRNPQDVDECAEALDSCSIDAICQNTLKSYKCICKSGYKGDGKHCEDIDECASEYNGGCVHECINIPGNYRCTCYDGFRLAHDGHNCLDVDECAEGNGGCQQICVNMMGSYECRCRDGFLLSDNQHTCIQRPKVRFNGTKDVATCMDKNHGCAHICRETPKGTIACECRPGFQLTKNNRDCKLTCNYGNGGCQHICEETDHGPKCSCHMKFALHSDGKTCVETCAVNNGGCDSTCHDAVTGVRCSCPVGFTLQPDRKTCKDIDECRMNNGGCDHVCRNTVGSFECSCKKGYKLLTNERTCQDIDECSFDRACDHTCINSPGSFQCYCHKGYVLYGVAHCGDIDECSINQGGCKSGCLNTLGSYECTCPPGYKLHWNKKDCVELIKCASNLVAPKATLACSKTGKKESCSLTCASKAHYLSESDSSYSVSCGISILRGKSQSRVNTSSIQSCIETQVHPVKQTASFRIKNAKCHLHPKHKGRAEDNGRQIGPGGQPCNNCLVTFVNLKCDSSKKTKGGRRARNPTNKEVTRITLELEAEVKPGDATGNCNMMCQRQHMEQQMKTYIKTLKKSINQERFLLRVAGLEYEVAQKLTQTALKQENCGPGREKDSGRCVRCLPGSYYHGEQERCIQCPPGTFQEREGQLACDLCPGGDRHGPQGARNITSCAGQCPPGHFSSDGFKPCQPCPLGKYQPDPGRTLCFSCGGGLSTKREGASSFNDCEVKVLCSPGHYYNTSVHRCIRCPHGTYQAEFRQNYCISCPGNTTTDFDGAISVSQCKNRECGGEIGEFIGYIESPNYPGNYPANVECVWTINPPHKRKILIVVPEIFLPSEDECGDVLVMRKNWSATSITTYETCQTYERPIAFTARSRRLWINFKSNDVNSARGFQIPYVTYDEDYEQLVEDIVRDGRLYASENHQEILKDKKLIKTLFDVLAHPHNYYKYSTRDSTEMLPRSFIRLIHSKVSAFLRPYN

Target ORF information:

RefSeq Version	NM_001260501.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio signal peptide, CUB domain, EGF-like 3 (scube3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260501.1

ORF Insert Sequence:

ATGCGCGCTG GTTTTGGATT ATTCAGCGTC GCTCTGGTCG CCTTTGTCCA GGGTCCAGCC 
GGTGTTTTTG CCAGCAGAAA CCCGCAAGAT GTGGACGAGT GCGCCGAGGC CCTGGACAGC 
TGCAGTATAG ATGCCATCTG TCAGAACACC CTCAAGTCAT ACAAATGCAT CTGCAAATCA 
GGATACAAAG GCGACGGGAA ACACTGTGAA GACATCGATG AGTGTGCCAG CGAATATAAT 
GGAGGATGTG TTCACGAGTG CATCAACATC CCTGGAAACT ACAGATGCAC GTGTTATGAC 
GGCTTCCGTC TGGCCCACGA CGGACACAAC TGCTTGGATG TGGATGAATG TGCTGAAGGA 
AACGGCGGCT GTCAGCAGAT CTGTGTTAAC ATGATGGGCA GTTATGAATG TCGCTGCAGA 
GACGGCTTTC TGCTGTCCGA CAACCAGCAC ACCTGCATCC AGAGGCCCAA AGTACGTTTC 
AATGGAACTA AGGATGTGGC CACCTGCATG GATAAAAATC ACGGCTGTGC CCATATTTGC 
CGTGAGACCC CTAAAGGAAC CATCGCCTGT GAATGCAGAC CAGGATTTCA GCTCACCAAG 
AACAATCGAG ACTGTAAATT GACCTGTAAC TATGGTAATG GCGGCTGTCA GCACATCTGT 
GAGGAAACGG ATCATGGGCC CAAATGCTCG TGTCACATGA AGTTTGCTCT TCACTCAGAT 
GGAAAGACCT GTGTAGAGAC GTGTGCAGTG AATAACGGTG GTTGTGACAG CACGTGTCAT 
GATGCTGTGA CTGGTGTTCG CTGCAGCTGC CCGGTGGGAT TCACTTTACA GCCCGACCGC 
AAGACCTGCA AAGACATTGA TGAATGTCGC ATGAACAACG GTGGCTGTGA TCACGTTTGC 
AGAAACACCG TCGGCAGCTT CGAGTGCAGC TGCAAGAAAG GATACAAACT CCTGACCAAT 
GAGAGGACGT GCCAAGATAT AGACGAGTGT TCGTTTGACC GGGCCTGTGA CCACACATGC 
ATAAATTCAC CCGGGAGTTT CCAATGCTAC TGTCATAAAG GCTACGTCCT CTACGGCGTG 
GCTCACTGCG GAGATATCGA CGAGTGCAGC ATTAATCAGG GTGGCTGTAA ATCGGGCTGT 
CTTAACACGC TGGGCAGTTA TGAATGTACC TGTCCTCCTG GATACAAACT GCACTGGAAC 
AAGAAGGACT GTGTGGAGCT CATAAAATGT GCCTCCAATT TGGTGGCCCC CAAAGCCACC 
CTGGCCTGCA GTAAAACGGG CAAGAAAGAG AGCTGTTCCC TCACGTGTGC ATCCAAAGCA 
CATTATCTGT CAGAATCAGA CAGCAGTTAT TCTGTCAGCT GTGGAATATC AATCCTGCGA 
GGAAAATCTC AGAGCAGAGT CAACACGAGC AGCATCCAGA GCTGCATAGA GACTCAGGTT 
CATCCAGTGA AGCAAACGGC GTCTTTTCGG ATCAAGAACG CCAAATGTCA CCTGCACCCG 
AAGCATAAGG GGAGAGCAGA GGACAACGGC AGACAGATCG GCCCAGGCGG TCAGCCGTGC 
AACAACTGCC TGGTCACGTT TGTTAACCTG AAGTGTGACT CCTCTAAGAA GACCAAAGGC 
GGTCGTCGAG CTCGAAACCC GACAAATAAA GAGGTGACGC GCATTACTCT GGAGCTGGAG 
GCCGAGGTCA AACCGGGAGA CGCAACCGGG AACTGCAACA TGATGTGTCA ACGGCAGCAC 
ATGGAGCAGC AGATGAAGAC GTACATTAAA ACCCTGAAGA AGTCCATTAA CCAGGAGCGT 
TTCCTGCTGC GTGTCGCTGG ACTGGAGTAT GAGGTGGCAC AGAAGCTCAC ACAGACAGCG 
TTGAAGCAGG AGAACTGCGG ACCGGGCCGA GAAAAGGACA GCGGACGTTG TGTCAGATGT 
TTGCCTGGCT CATATTACCA TGGGGAGCAG GAGCGATGCA TCCAGTGCCC ACCGGGAACC 
TTCCAGGAGC GGGAAGGTCA GCTTGCCTGC GATCTGTGTC CTGGAGGAGA CCGGCATGGC 
CCACAAGGAG CCCGCAACAT CACGTCATGT GCAGGTCAGT GTCCACCAGG GCATTTCTCC 
AGCGACGGGT TCAAGCCCTG TCAGCCGTGT CCATTGGGAA AATACCAGCC AGATCCAGGA 
CGGACCCTCT GTTTCTCCTG TGGAGGTGGA CTCAGTACTA AAAGAGAGGG CGCCAGTTCA 
TTCAATGACT GCGAGGTCAA AGTGTTGTGT TCTCCAGGTC ATTATTATAA CACATCAGTT 
CATCGGTGTA TCCGCTGTCC TCATGGAACC TACCAGGCTG AATTCAGACA GAACTACTGC 
ATCTCCTGTC CTGGAAACAC CACCACAGAC TTTGACGGAG CCATCAGTGT GTCTCAGTGC 
AAGAATCGCG AATGCGGGGG TGAAATCGGC GAGTTCATTG GATACATCGA GTCACCCAAC 
TATCCCGGAA ACTACCCAGC CAATGTGGAG TGTGTGTGGA CCATCAACCC TCCACACAAA 
CGCAAGATTC TGATTGTGGT TCCTGAGATC TTCCTCCCGT CTGAGGATGA GTGTGGAGAT 
GTGCTGGTCA TGAGGAAGAA CTGGTCTGCA ACCTCTATTA CCACCTATGA GACGTGTCAG 
ACATACGAGA GACCGATTGC ATTTACAGCC CGATCGAGGC GCCTCTGGAT CAACTTCAAA 
TCCAATGATG TGAACAGCGC TCGTGGTTTC CAGATTCCAT ACGTCACTTA TGATGAAGAC 
TATGAGCAGC TGGTTGAAGA TATCGTAAGA GACGGACGGC TTTATGCCTC TGAAAACCAT 
CAAGAAATAC TCAAGGATAA AAAACTGATT AAAACACTGT TCGACGTTCT GGCTCACCCT 
CACAATTACT ACAAGTACTC GACTCGAGAC TCCACGGAGA TGCTCCCGCG CTCCTTCATT 
CGCCTCATCC ACAGCAAAGT CTCGGCCTTC CTACGTCCGT ACAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scube3 ( XM_021469709.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021325384.1 Danio rerio signal peptide, CUB domain, EGF-like 3 (scube3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa151413
Clone ID Related Accession (Same CDS sequence)	XM_021469709.1
Accession Version	XM_021469709.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	signal peptide, CUB and EGF-like domain-containing protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTTCA TGCATGTTCT GATGCGCGCT GGTTTTGGAT TATTCAGCGT CGCTCTGGTC 
GCCTTTGTCC AGGGTCCAGC CGGTGTTTTT GCCAGCAGAA ACCCGCAAGA TGTGGACGAG 
TGCGCCGAGG CCCTGGACAG CTGCAGTATA GATGCCATCT GTCAGAACAC CCTCAAGTCA 
TACAAATGCA TCTGCAAATC AGGATACAAA GGCGACGGGA AACACTGTGA AGACATCGAT 
GAGTGTGCCA GCGAATATAA TGGAGGATGT GTTCACGAGT GCATCAACAT CCCTGGAAAC 
TACAGATGCA CGTGTTATGA CGGCTTCCGT CTGGCCCACG ACGGACACAA CTGCTTGGAT 
GTGGATGAAT GTGCTGAAGG AAACGGCGGC TGTCAGCAGA TCTGTGTTAA CATGATGGGC 
AGTTATGAAT GTCGCTGCAG AGACGGCTTT CTGCTGTCCG ACAACCAGCA CACCTGCATC 
CAGAGGCCCA AAGTACGTTT CAATGGGACT AAGGATGTGG CCACCTGCAT GGATAAAAAT 
CACGGCTGTG CCCATATTTG CCGTGAGACC CCTAAAGGAA CCATCGCCTG TGAATGCAGA 
CCAGGATTTC AGCTCACCAA GAACAATCGA GACTGTAAAT TGACCTGTAA CTATGGTAAT 
GGCGGCTGTC AGCACATCTG TGAGGAAACG GATCATGGGC CCAAATGCTC GTGTCACATG 
AAGTTTGCTC TTCACTCAGA TGGAAAGACC TGTGTAGAGG AAAGGAGTGC CCAACATCAG 
CCACTGCAGC CACAGCTGTT ACCCAATGAG ACGTGTGCAG TGAATAACGG TGGTTGTGAC 
AGCACGTGTC ATGATGCTGT GACTGGTGTT CGCTGCAGCT GCCCGGTGGG ATTCACTTTA 
CAGCCCGACC GCAAGACCTG CAAAGACATT GATGAATGTC GCATGAACAA CGGTGGCTGT 
GATCACGTTT GCAGAAACAC CGTCGGCAGC TTCGAGTGCA GCTGCAAGAA AGGATACAAA 
CTCCTGACCA ATGAGAGGAC GTGCCAAGAT ATAGACGAGT GTTCGTTTGA CCGGGCCTGT 
GACCACACAT GCATAAATTC ACCCGGGAGT TTCCAATGCT ACTGTCATAA AGGCTACGTC 
CTCTACGGCG TGGCTCACTG CGGAGATATC GACGAGTGCA GCATTAATCA GGGTGGCTGT 
AAATCGGGCT GTCTTAACAC GCTGGGCAGT TATGAATGTA CCTGTCCTCC TGGATACAAA 
CTGCACTGGA ACAAGAAGGA CTGTGTGGAG CTCATAAAAT GTGCCTCCAA TTTGGTGGCC 
CCCAAAGCCA CCCTGGCCTG CAGTAAAACG GGCAAGAAAG AGAGCTGTTC CCTCACGTGT 
GCATCCAAAG CACATTATCT GTCAGAATCA GACAGCAGTT ATTCTGTCAG CTGTGGAATA 
TCAATCCTGC GAGGAAAATC TCAGAGCAGA GTCAACACGA GCAGCATCCA GAGCTGCATA 
GAGACTCAGG TTCATCCAGT GAAGCAAACG GCGTCTTTTC GGATCAAGAA CGCCAAATGT 
CACCTGCACC CGAAGCATAA GGGGAGAGCA GAGGACAACG GCAGACAGAT CGGCCCAGGC 
GGTCAGCCGT GCAACAACTG CCTGGTCACG TTTGTTAACC TGAAGTGTGA CTCCTCTAAG 
AAGACCAAAG GCGGTCGTCG AGCTCGAAAC CCGACAAATA AAGAGGTGAC GCGCATTACT 
CTGGAGCTGG AGGCCGAGGT CAAACCGGGA GACGCAACCG GGAACTGCAA CATGATGTGT 
CAACGGCAGC ACATGGAGCA GCAGATGAAG ACGTACATTA AAACCCTGAA GAAGTCCATT 
AACCAGGAGC GTTTCCTGCT GCGTGTCGCT GGACTGGAGT ATGAGGTGGC ACAGAAGCTC 
ACACAGACAG CGTTGAAGCA GGAGAACTGC GGACCGGGCC GAGAAAAGGA CAGCGGACGT 
TGTGTCAGAT GTTTGCCTGG CTCATATTAC CATGGGGAGC AGGAGCGATG CATCCAGTGC 
CCACCGGGAA CCTTCCAGGA GCGGGAAGGT CAGCTTGCCT GCGATCTGTG TCCTGGAGGA 
GACCGGCATG GCCCACAAGG AGCCCGCAAC ATCACGTCAT GTGCAGGTCA GTGTCCACCA 
GGGCATTTCT CCAGCGACGG GTTCAAGCCC TGTCAGCCGT GTCCATTGGG AAAATACCAG 
CCAGATCCAG GACGGACCCT CTGTTTCTCC TGTGGAGGTG GACTCAGTAC TAAAAGAGAG 
GGCGCCAGTT CATTCAATGA CTGCGAGGTC AAAGTGTTGT GTTCTCCAGG TCATTATTAT 
AACACATCAG TTCATCGGTG TATCCGCTGT CCTCATGGAA CCTACCAGGC TGAATTCAGA 
CAGAACTACT GCATCTCCTG TCCTGGAAAC ACCACCACAG ACTTTGACGG AGCCATCAGT 
GTGTCTCAGT GCAAGAATCG CGAATGCGGG GGTGAAATCG GCGAGTTCAT TGGATACATC 
GAGTCACCCA ACTATCCCGG AAACTACCCA GCCAATGTGG AGTGTGTGTG GACCATCAAC 
CCTCCACACA AACGCAAGAT TCTGATTGTG GTTCCTGAGA TCTTCCTCCC GTCTGAGGAT 
GAGTGTGGAG ATGTGCTGGT CATGAGGAAG AACTGGTCTG CAACCTCTAT TACCACCTAT 
GAGACGTGTC AGACATACGA GAGACCGATT GCATTTACAG CCCGATCGAG GCGCCTCTGG 
ATCAACTTCA AATCCAATGA TGTGAACAGC GCTCGTGGTT TCCAGATTCC ATACGTCACT 
TATGATGAAG ACTATGAGCA GCTGGTTGAA GATATCGTAA GAGACGGACG GCTTTATGCC 
TCTGAAAACC ATCAAGAAAT ACTCAAGGAT AAAAAACTGA TTAAAACACT GTTCGACGTT 
CTGGCTCACC CTCACAATTA CTACAAGTAC TCGACTCGAG ACTCCACGGA GATGCTCCCG 
CGCTCCTTCA TTCGCCTCAT CCACAGCAAA GTCTCGGCCT TCCTACGTCC GTACAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325384.1
CDS	227..3286
Translation	MMFMHVLMRAGFGLFSVALVAFVQGPAGVFASRNPQDVDECAEALDSCSIDAICQNTLKSYKCICKSGYKGDGKHCEDIDECASEYNGGCVHECINIPGNYRCTCYDGFRLAHDGHNCLDVDECAEGNGGCQQICVNMMGSYECRCRDGFLLSDNQHTCIQRPKVRFNGTKDVATCMDKNHGCAHICRETPKGTIACECRPGFQLTKNNRDCKLTCNYGNGGCQHICEETDHGPKCSCHMKFALHSDGKTCVEERSAQHQPLQPQLLPNETCAVNNGGCDSTCHDAVTGVRCSCPVGFTLQPDRKTCKDIDECRMNNGGCDHVCRNTVGSFECSCKKGYKLLTNERTCQDIDECSFDRACDHTCINSPGSFQCYCHKGYVLYGVAHCGDIDECSINQGGCKSGCLNTLGSYECTCPPGYKLHWNKKDCVELIKCASNLVAPKATLACSKTGKKESCSLTCASKAHYLSESDSSYSVSCGISILRGKSQSRVNTSSIQSCIETQVHPVKQTASFRIKNAKCHLHPKHKGRAEDNGRQIGPGGQPCNNCLVTFVNLKCDSSKKTKGGRRARNPTNKEVTRITLELEAEVKPGDATGNCNMMCQRQHMEQQMKTYIKTLKKSINQERFLLRVAGLEYEVAQKLTQTALKQENCGPGREKDSGRCVRCLPGSYYHGEQERCIQCPPGTFQEREGQLACDLCPGGDRHGPQGARNITSCAGQCPPGHFSSDGFKPCQPCPLGKYQPDPGRTLCFSCGGGLSTKREGASSFNDCEVKVLCSPGHYYNTSVHRCIRCPHGTYQAEFRQNYCISCPGNTTTDFDGAISVSQCKNRECGGEIGEFIGYIESPNYPGNYPANVECVWTINPPHKRKILIVVPEIFLPSEDECGDVLVMRKNWSATSITTYETCQTYERPIAFTARSRRLWINFKSNDVNSARGFQIPYVTYDEDYEQLVEDIVRDGRLYASENHQEILKDKKLIKTLFDVLAHPHNYYKYSTRDSTEMLPRSFIRLIHSKVSAFLRPYN

Target ORF information:

RefSeq Version	XM_021469709.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio signal peptide, CUB domain, EGF-like 3 (scube3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469709.1

ORF Insert Sequence:

ATGATGTTCA TGCATGTTCT GATGCGCGCT GGTTTTGGAT TATTCAGCGT CGCTCTGGTC 
GCCTTTGTCC AGGGTCCAGC CGGTGTTTTT GCCAGCAGAA ACCCGCAAGA TGTGGACGAG 
TGCGCCGAGG CCCTGGACAG CTGCAGTATA GATGCCATCT GTCAGAACAC CCTCAAGTCA 
TACAAATGCA TCTGCAAATC AGGATACAAA GGCGACGGGA AACACTGTGA AGACATCGAT 
GAGTGTGCCA GCGAATATAA TGGAGGATGT GTTCACGAGT GCATCAACAT CCCTGGAAAC 
TACAGATGCA CGTGTTATGA CGGCTTCCGT CTGGCCCACG ACGGACACAA CTGCTTGGAT 
GTGGATGAAT GTGCTGAAGG AAACGGCGGC TGTCAGCAGA TCTGTGTTAA CATGATGGGC 
AGTTATGAAT GTCGCTGCAG AGACGGCTTT CTGCTGTCCG ACAACCAGCA CACCTGCATC 
CAGAGGCCCA AAGTACGTTT CAATGGGACT AAGGATGTGG CCACCTGCAT GGATAAAAAT 
CACGGCTGTG CCCATATTTG CCGTGAGACC CCTAAAGGAA CCATCGCCTG TGAATGCAGA 
CCAGGATTTC AGCTCACCAA GAACAATCGA GACTGTAAAT TGACCTGTAA CTATGGTAAT 
GGCGGCTGTC AGCACATCTG TGAGGAAACG GATCATGGGC CCAAATGCTC GTGTCACATG 
AAGTTTGCTC TTCACTCAGA TGGAAAGACC TGTGTAGAGG AAAGGAGTGC CCAACATCAG 
CCACTGCAGC CACAGCTGTT ACCCAATGAG ACGTGTGCAG TGAATAACGG TGGTTGTGAC 
AGCACGTGTC ATGATGCTGT GACTGGTGTT CGCTGCAGCT GCCCGGTGGG ATTCACTTTA 
CAGCCCGACC GCAAGACCTG CAAAGACATT GATGAATGTC GCATGAACAA CGGTGGCTGT 
GATCACGTTT GCAGAAACAC CGTCGGCAGC TTCGAGTGCA GCTGCAAGAA AGGATACAAA 
CTCCTGACCA ATGAGAGGAC GTGCCAAGAT ATAGACGAGT GTTCGTTTGA CCGGGCCTGT 
GACCACACAT GCATAAATTC ACCCGGGAGT TTCCAATGCT ACTGTCATAA AGGCTACGTC 
CTCTACGGCG TGGCTCACTG CGGAGATATC GACGAGTGCA GCATTAATCA GGGTGGCTGT 
AAATCGGGCT GTCTTAACAC GCTGGGCAGT TATGAATGTA CCTGTCCTCC TGGATACAAA 
CTGCACTGGA ACAAGAAGGA CTGTGTGGAG CTCATAAAAT GTGCCTCCAA TTTGGTGGCC 
CCCAAAGCCA CCCTGGCCTG CAGTAAAACG GGCAAGAAAG AGAGCTGTTC CCTCACGTGT 
GCATCCAAAG CACATTATCT GTCAGAATCA GACAGCAGTT ATTCTGTCAG CTGTGGAATA 
TCAATCCTGC GAGGAAAATC TCAGAGCAGA GTCAACACGA GCAGCATCCA GAGCTGCATA 
GAGACTCAGG TTCATCCAGT GAAGCAAACG GCGTCTTTTC GGATCAAGAA CGCCAAATGT 
CACCTGCACC CGAAGCATAA GGGGAGAGCA GAGGACAACG GCAGACAGAT CGGCCCAGGC 
GGTCAGCCGT GCAACAACTG CCTGGTCACG TTTGTTAACC TGAAGTGTGA CTCCTCTAAG 
AAGACCAAAG GCGGTCGTCG AGCTCGAAAC CCGACAAATA AAGAGGTGAC GCGCATTACT 
CTGGAGCTGG AGGCCGAGGT CAAACCGGGA GACGCAACCG GGAACTGCAA CATGATGTGT 
CAACGGCAGC ACATGGAGCA GCAGATGAAG ACGTACATTA AAACCCTGAA GAAGTCCATT 
AACCAGGAGC GTTTCCTGCT GCGTGTCGCT GGACTGGAGT ATGAGGTGGC ACAGAAGCTC 
ACACAGACAG CGTTGAAGCA GGAGAACTGC GGACCGGGCC GAGAAAAGGA CAGCGGACGT 
TGTGTCAGAT GTTTGCCTGG CTCATATTAC CATGGGGAGC AGGAGCGATG CATCCAGTGC 
CCACCGGGAA CCTTCCAGGA GCGGGAAGGT CAGCTTGCCT GCGATCTGTG TCCTGGAGGA 
GACCGGCATG GCCCACAAGG AGCCCGCAAC ATCACGTCAT GTGCAGGTCA GTGTCCACCA 
GGGCATTTCT CCAGCGACGG GTTCAAGCCC TGTCAGCCGT GTCCATTGGG AAAATACCAG 
CCAGATCCAG GACGGACCCT CTGTTTCTCC TGTGGAGGTG GACTCAGTAC TAAAAGAGAG 
GGCGCCAGTT CATTCAATGA CTGCGAGGTC AAAGTGTTGT GTTCTCCAGG TCATTATTAT 
AACACATCAG TTCATCGGTG TATCCGCTGT CCTCATGGAA CCTACCAGGC TGAATTCAGA 
CAGAACTACT GCATCTCCTG TCCTGGAAAC ACCACCACAG ACTTTGACGG AGCCATCAGT 
GTGTCTCAGT GCAAGAATCG CGAATGCGGG GGTGAAATCG GCGAGTTCAT TGGATACATC 
GAGTCACCCA ACTATCCCGG AAACTACCCA GCCAATGTGG AGTGTGTGTG GACCATCAAC 
CCTCCACACA AACGCAAGAT TCTGATTGTG GTTCCTGAGA TCTTCCTCCC GTCTGAGGAT 
GAGTGTGGAG ATGTGCTGGT CATGAGGAAG AACTGGTCTG CAACCTCTAT TACCACCTAT 
GAGACGTGTC AGACATACGA GAGACCGATT GCATTTACAG CCCGATCGAG GCGCCTCTGG 
ATCAACTTCA AATCCAATGA TGTGAACAGC GCTCGTGGTT TCCAGATTCC ATACGTCACT 
TATGATGAAG ACTATGAGCA GCTGGTTGAA GATATCGTAA GAGACGGACG GCTTTATGCC 
TCTGAAAACC ATCAAGAAAT ACTCAAGGAT AAAAAACTGA TTAAAACACT GTTCGACGTT 
CTGGCTCACC CTCACAATTA CTACAAGTAC TCGACTCGAG ACTCCACGGA GATGCTCCCG 
CGCTCCTTCA TTCGCCTCAT CCACAGCAAA GTCTCGGCCT TCCTACGTCC GTACAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Whole exome sequencing coupled with unbiased functional analysis reveals new Hirschsprung disease genes.
Genome biology18(1)48(2017 Mar)
Gui H,Schriemer D,Cheng WW,Chauhan RK,Anti?olo G,Berrios C,Bleda M,Brooks AS,Brouwer RW,Burns AJ,Cherny SS,Dopazo J,Eggen BJ,Griseri P,Jalloh B,Le TL,Lui VC,Luz?n-Toro B,Matera I,Ngan ES,Pelet A,Ruiz-Ferrer M,Sham PC,Shepherd IT,So MT,Sribudiani Y,Tang CS,van den Hout MC,van der Linde HC,van Ham TJ,van IJcken WF,Verheij JB,Amiel J,Borrego S,Ceccherini I,Chakravarti A,Lyonnet S,Tam PK,Garcia-Barcel? MM,Hofstra RM

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Molecular cloning, tissue expression pattern, and copy number variation of porcine SCUBE3.
Genetics and molecular research : GMR15(1)(2016 Feb)
Liu X,Wang LG,Zhang LC,Yan H,Zhao KB,Liang J,Li N,Pu L,Zhang T,Wang LX

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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scube3 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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