ppwd1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ppwd1
Entrez Gene ID	100000660
Full Name	peptidylprolyl isomerase domain and WD repeat containing 1
Synonyms	zgc:165355
General protein information	Preferred Names peptidylprolyl isomerase domain and WD repeat containing 1 Names peptidylprolyl isomerase domain and WD repeat-containing protein 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001098758.1	NP_001092228.1	peptidylprolyl isomerase domain and WD repeat-containing protein 1
XM_021479088.1	XP_021334763.1	peptidylprolyl isomerase domain and WD repeat-containing protein 1 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ppwd1	NP_766395.2
Caenorhabditis elegans (roundworm)	cyn-15	NP_493378.1
Schizosaccharomyces pombe (fission yeast)	cyp9	NP_587769.1
Magnaporthe oryzae (rice blast fungus)	MGG_05731	XP_003710641.1
Homo sapiens (human)	PPWD1	NP_056157.1
Pan troglodytes (chimpanzee)	PPWD1	XP_001164190.1
	PPWD1	XP_001085845.1
Bos taurus (cattle)	PPWD1	NP_001039696.1
Gallus gallus (chicken)	PPWD1	XP_424751.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008660	XP_314761.4
Arabidopsis thaliana (thale cress)	CYP71	NP_190046.2
Xenopus tropicalis (tropical clawed frog)	ppwd1	XP_002934239.2
Canis lupus familiaris (dog)	PPWD1	XP_535256.2
Rattus norvegicus (Norway rat)	Ppwd1	NP_001099876.1
Danio rerio (zebrafish)	ppwd1	NP_001092228.1
Neurospora crassa	NCU09819	XP_963759.1
Drosophila melanogaster (fruit fly)	CG3511	NP_611935.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Many genes in fish have species-specific asymmetric rates of molecular evolution.
Steinke D, Salzburger W, Braasch I, Meyer A
BMC genomics720(2006 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ppwd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ppwd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa33909	NM_001098758.1 Latest version!	Danio rerio peptidylprolyl isomerase domain and WD repeat containing 1 (ppwd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa147572	XM_021479088.1 Latest version!	Danio rerio peptidylprolyl isomerase domain and WD repeat containing 1 (ppwd1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ppwd1 ( NM_001098758.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001092228.1 Danio rerio peptidylprolyl isomerase domain and WD repeat containing 1 (ppwd1), mRNA.

Price & Availability↑

CloneID	ODa33909
Clone ID Related Accession (Same CDS sequence)	NM_001098758.1
Accession Version	NM_001098758.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	peptidylprolyl isomerase domain and WD repeat-containing protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC142740.1. On Jun 20, 2007 this sequence version replaced XM_001340777.1. ##Evidence-Data-START## Transcript exon combination :: BC142740.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCGACCG ACGTGAGCAG TGATGGTTCT GAGAATAAAC GAAAACTGGA AGAAGATGAA 
GACGAAGAGA ATTGGGTTGG TCCTATGCCG AGTGAGGCCA CACAAACCAA AAAGAAAAAA 
GTTCTTGAGT TTGAGAGATT GTACCTGGAT AATTTACCAT CTGCCTCCAT GTATGAAAGA 
AGTTACATGC ACAGAGATGT TATAACACAC ATTGTTTGCT CCAAAACTGA TTTTATCCTC 
ACAGCAAGTC AAGATGGACA TGTCAAATTC TGGAAGAAGA AAGAGGATGA AGGAGTGGAG 
TTTGTCAAGC ACTTCCGCAG CCACTTGGGA GTGATTGAGT GCATTTCCGT GAGTGCGGAG 
GGAGCACTTT TATGCTCTGT TGGTGATGAT CAAGCGATGA AAGTTTTTGA TGTAGTCAAC 
TTTGACATGA TCAACATGCT CAAACTTGGC TTCCATCCAG GCCAGTGTGA GTGGATTTAT 
AATCCAGGAG ACGCCATTTC AACAATCGCC TGCTCTGAGA AATCCACTGG AAAGATTTTT 
GTGTATGATG GACGTGGCAG TAATCAGCCC CTGCACGTAT TCGAAAAGAT GCACACCACA 
CCACTCTCTC AGATCCGCCT CAATCCCAAA TATCGTGTCA TCGTATCTGC CGATAAAGCG 
GGAATGCTAG AATACTGGAC GGGGCTCCCG AGCGAATTCA AATTTCCGCG GCAGGTGGAC 
TGGGAGTTTA AGACCGACAC AGACTTGTAT GAGTTTGCCA AATGTGGAAC GTATCCCACC 
AGCCTGGCTT TCTCTCAAGA TGGCAAGAAA ATGGCCACCA TTGCCGTTGA CCGCAAAGTG 
AGGATTTTCC GCTTTCTCAC TGGGAAGTTG ATGCGGGTGT TTGACGAATC ACTAACAATG 
TTTACAGAGC TCCAGCAGAT GAGACAGCAG TTGCCTGATA TGGAGTTTGG TCGCAGAATG 
GCAGTGGAGA GAGAGCTGGA GAAGGTGGAC GGCATACGAT TGACCAACAT CATCTTTGAT 
GAGACTGGAC ACTTTGTGCT TTACGGCACC ATGCTAGGCA TTAAAGTCAT CAACGTAGAG 
ACTAACAGGT GTGTGCGCAT ACTGGGCAAG CAGGAGAACA TCCGTGTGGT GCAGTTGAGT 
CTGTTTCAGG GAGTCGCAAA GGCCATGCAA GCGGCACCAA CAATTGAGAT GAAAGCCTCT 
GAAAACCCAG CACTCCAGAG CTCTGACCCA GACCCCACCA TCTTCTGCAC TGCCTTTAAG 
AAGAACCGCT TTTACATGTT TACAAAAAGA GAGCCCGAGG ACACAAAGAG TGCTGATTCT 
GACCGTGATA TTTTCAACGA GAAGCCGTCT AAAGAGGAAG TGATGGCAGC CACACAGGCG 
GAAGGACCAA AAAGAGTGTC AGACAGTGCC ATCATTCACA CCACTATGGG TGACATTCAC 
ATCAAACTCT TTCCTGTCGA ATGCCCCAAA ACTGTGGAGA ACTTCTGTGT TCACAGCAGG 
AACGGTTATT ACAACGGCCA CATTTTTCAC CGAGTCATCA AGGGTTTCAT GATCCAGACT 
GGAGACCCCA CAGGGACTGG CATGGGTGGG GAAAGCATCT GGGGTGGAGA GTTTGAGGAT 
GAGTTTCATT CCACTCTGAG ACATGACAGA CCCTACACCC TCAGCATGGC CAATGCAGGA 
CCAGGAACCA ACGGCTCACA GTTCTTCATC ACTGTCGTCC CAACCCCCTG GCTGGACAAT 
AAGCACACAG TGTTTGGAAG GACCTCTAAA GGGATGGAGG TGGTCCAGAG GATCTCCAAC 
ATAAAAGTCA ACCCCAAAAC CGACAAACCA TATGAAGACA TCAGCATCAT TAATATCACT 
GTCAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001092228.1
CDS	17..1885
Translation	MATDVSSDGSENKRKLEEDEDEENWVGPMPSEATQTKKKKVLEFERLYLDNLPSASMYERSYMHRDVITHIVCSKTDFILTASQDGHVKFWKKKEDEGVEFVKHFRSHLGVIECISVSAEGALLCSVGDDQAMKVFDVVNFDMINMLKLGFHPGQCEWIYNPGDAISTIACSEKSTGKIFVYDGRGSNQPLHVFEKMHTTPLSQIRLNPKYRVIVSADKAGMLEYWTGLPSEFKFPRQVDWEFKTDTDLYEFAKCGTYPTSLAFSQDGKKMATIAVDRKVRIFRFLTGKLMRVFDESLTMFTELQQMRQQLPDMEFGRRMAVERELEKVDGIRLTNIIFDETGHFVLYGTMLGIKVINVETNRCVRILGKQENIRVVQLSLFQGVAKAMQAAPTIEMKASENPALQSSDPDPTIFCTAFKKNRFYMFTKREPEDTKSADSDRDIFNEKPSKEEVMAATQAEGPKRVSDSAIIHTTMGDIHIKLFPVECPKTVENFCVHSRNGYYNGHIFHRVIKGFMIQTGDPTGTGMGGESIWGGEFEDEFHSTLRHDRPYTLSMANAGPGTNGSQFFITVVPTPWLDNKHTVFGRTSKGMEVVQRISNIKVNPKTDKPYEDISIINITVK

Target ORF information:

RefSeq Version	NM_001098758.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio peptidylprolyl isomerase domain and WD repeat containing 1 (ppwd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001098758.1

ORF Insert Sequence:

ATGGCGACCG ACGTGAGCAG TGATGGTTCT GAGAATAAAC GAAAACTGGA AGAAGATGAA 
GACGAAGAGA ATTGGGTTGG TCCTATGCCG AGTGAGGCCA CACAAACCAA AAAGAAAAAA 
GTTCTTGAGT TTGAGAGATT GTACCTGGAT AATTTACCAT CTGCCTCCAT GTATGAAAGA 
AGTTACATGC ACAGAGATGT TATAACACAC ATTGTTTGCT CCAAAACTGA TTTTATCCTC 
ACAGCAAGTC AAGATGGACA TGTCAAATTC TGGAAGAAGA AAGAGGATGA AGGAGTGGAG 
TTTGTCAAGC ACTTCCGCAG CCACTTGGGA GTGATTGAGT GCATTTCCGT GAGTGCGGAG 
GGAGCACTTT TATGCTCTGT TGGTGATGAT CAAGCGATGA AAGTTTTTGA TGTAGTCAAC 
TTTGACATGA TCAACATGCT CAAACTTGGC TTCCATCCAG GCCAGTGTGA GTGGATTTAT 
AATCCAGGAG ACGCCATTTC AACAATCGCC TGCTCTGAGA AATCCACTGG AAAGATTTTT 
GTGTATGATG GACGTGGCAG TAATCAGCCC CTGCACGTAT TCGAAAAGAT GCACACCACA 
CCACTCTCTC AGATCCGCCT CAATCCCAAA TATCGTGTCA TCGTATCTGC CGATAAAGCG 
GGAATGCTAG AATACTGGAC GGGGCTCCCG AGCGAATTCA AATTTCCGCG GCAGGTGGAC 
TGGGAGTTTA AGACCGACAC AGACTTGTAT GAGTTTGCCA AATGTGGAAC GTATCCCACC 
AGCCTGGCTT TCTCTCAAGA TGGCAAGAAA ATGGCCACCA TTGCCGTTGA CCGCAAAGTG 
AGGATTTTCC GCTTTCTCAC TGGGAAGTTG ATGCGGGTGT TTGACGAATC ACTAACAATG 
TTTACAGAGC TCCAGCAGAT GAGACAGCAG TTGCCTGATA TGGAGTTTGG TCGCAGAATG 
GCAGTGGAGA GAGAGCTGGA GAAGGTGGAC GGCATACGAT TGACCAACAT CATCTTTGAT 
GAGACTGGAC ACTTTGTGCT TTACGGCACC ATGCTAGGCA TTAAAGTCAT CAACGTAGAG 
ACTAACAGGT GTGTGCGCAT ACTGGGCAAG CAGGAGAACA TCCGTGTGGT GCAGTTGAGT 
CTGTTTCAGG GAGTCGCAAA GGCCATGCAA GCGGCACCAA CAATTGAGAT GAAAGCCTCT 
GAAAACCCAG CACTCCAGAG CTCTGACCCA GACCCCACCA TCTTCTGCAC TGCCTTTAAG 
AAGAACCGCT TTTACATGTT TACAAAAAGA GAGCCCGAGG ACACAAAGAG TGCTGATTCT 
GACCGTGATA TTTTCAACGA GAAGCCGTCT AAAGAGGAAG TGATGGCAGC CACACAGGCG 
GAAGGACCAA AAAGAGTGTC AGACAGTGCC ATCATTCACA CCACTATGGG TGACATTCAC 
ATCAAACTCT TTCCTGTCGA ATGCCCCAAA ACTGTGGAGA ACTTCTGTGT TCACAGCAGG 
AACGGTTATT ACAACGGCCA CATTTTTCAC CGAGTCATCA AGGGTTTCAT GATCCAGACT 
GGAGACCCCA CAGGGACTGG CATGGGTGGG GAAAGCATCT GGGGTGGAGA GTTTGAGGAT 
GAGTTTCATT CCACTCTGAG ACATGACAGA CCCTACACCC TCAGCATGGC CAATGCAGGA 
CCAGGAACCA ACGGCTCACA GTTCTTCATC ACTGTCGTCC CAACCCCCTG GCTGGACAAT 
AAGCACACAG TGTTTGGAAG GACCTCTAAA GGGATGGAGG TGGTCCAGAG GATCTCCAAC 
ATAAAAGTCA ACCCCAAAAC CGACAAACCA TATGAAGACA TCAGCATCAT TAATATCACT 
GTCAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ppwd1 ( XM_021479088.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334763.1 Danio rerio peptidylprolyl isomerase domain and WD repeat containing 1 (ppwd1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa147572
Clone ID Related Accession (Same CDS sequence)	XM_021479088.1
Accession Version	XM_021479088.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	peptidylprolyl isomerase domain and WD repeat-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACCG ACGTGAGCAG TGATGGTTCT GAGAATAAAC GAAAACTGGA AGAAGATGAA 
GACGAAGACA ATTGGGTTGG TCCTATGCCG AGTGAGGCCA CACAAACCAA AAAGAAAAAA 
GTTCTTGAGT TTGAGAGATT GTACCTGGAT AATTTACCAT CTGCCTCCAT GTATGAAAGA 
AGTTACATGC ACAGAGATGT TATAACACAC ATTGTTTGCT CCAAAACTGA TTTTATCCTC 
ACAGCAAGTC AAGATGGACA TGTCAAATTC TGGAAGAAGA AAGAGGATGA AGGAGTGGAG 
TTTGTCAAGC ACTTCCGCAG CCACTTGGGA GTGATTGAGT GCATTTCCGT GAGTGCGGAG 
GGAGCACTTT TATGCTCTGT TGGTGATGAT CAAGCGATGA AAGTTTTTGA TGTAGTCAAC 
TTTGACATGA TCAACATGCT CAAACTTGGC TTCCATCCAG GCCAGTGTGA GTGGATTTAT 
AATCCAGGAG ACGCCATTTC AACAATCGCC TGCTCTGAGA AATCCACTGG AAAGATTTTT 
GTGTATGATG GACGTGGCAG TAATCAGCCC CTGCACGTAT TCGAAAAGAT GCACACCACA 
CCACTCTCTC AGATCCGCCT CAATCCCAAA TATCGTGTCA TCGTATCTGC CGATAAAGCG 
GGAATGCTAG AATACTGGAC GGGGCTCCCG AGCGAATTCA AATTTCCGCG GCAGGTGGAC 
TGGGAGTTTA AGACCGACAC AGACTTGTAT GAGTTTGCCA AATGTGGAAC GTATCCCACC 
AGCCTGGCTT TCTCTCAAGA TGGCAAGAAA ATGGCCACCA TTGCCGTTGA CCGCAAAGTG 
AGGATTTTCC GCTTTCTCAC TGGGAAGTTG ATGCGGGTGT TTGACGAATC ACTAACAATG 
TTTACAGAGC TCCAGCAGAT GAGACAGCAG TTGCCTGATA TGGAGTTTGG TCGCAGAATG 
GCAGTGGAGA GAGAGCTGGA GAAGGTGGAC GGCATACGAT TGACCAACAT CATCTTTGAT 
GAGACTGGAC ACTTTGTGCT TTACGGCACC ATGCTAGGCA TTAAAGTCAT CAACGTAGAG 
ACTAACAGGT GTGTGCGCAT ACTGGGCAAG CAGGAGAACA TCCGTGTGGT GCAGTTGAGT 
CTGTTTCAGG GAGTCGCAAA GGCCATGCAA GCGGCACCAA CAATTGAGAT GAAAGCCTCT 
GAAAACCCAG CACTCCAGAG CTCTGACCCA GACCCCACCA TCTTCTGCAC TGCCTTTAAG 
AAGAACCGCT TTTACATGTT TACAAAAAGA GAGCCCGAGG ACACAAAGAG TGCTGATTCT 
GACCGTGATA TTTTCAACGA GAAGCCGTCT AAAGAGGAAG TGATGGCAGC CACACAGGCG 
GAAGGACCAA AAAGAGTGTC AGACAGTGCC ATCATTCACA CCACTATGGG TGACATTCAC 
ATCAAACTCT TTCCTGTCGA ATGCCCCAAA ACTGTGGAGA ACTTCTGTGT TCACAGCAGG 
AACGGTTATT ACAACGGCCA CATTTTTCAC CGAGTCATCA AGGGTTTCAT GATCCAGACT 
GGAGACCCCA CAGGGACTGG CATGGGTGGG GAAAGCATCT GGGGTGGAGA GTTTGAGGAT 
GAGTTTCATT CCACTCTGAG ACATGACAGA CCCTACACCC TCAGCATGGC CAATGCAGGA 
CCAGGAACCA ACGGCTCACA GTTCTTCATC ACTGTCGTCC CAACCGTAAG ACCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334763.1
CDS	37..1773
Translation	MATDVSSDGSENKRKLEEDEDEDNWVGPMPSEATQTKKKKVLEFERLYLDNLPSASMYERSYMHRDVITHIVCSKTDFILTASQDGHVKFWKKKEDEGVEFVKHFRSHLGVIECISVSAEGALLCSVGDDQAMKVFDVVNFDMINMLKLGFHPGQCEWIYNPGDAISTIACSEKSTGKIFVYDGRGSNQPLHVFEKMHTTPLSQIRLNPKYRVIVSADKAGMLEYWTGLPSEFKFPRQVDWEFKTDTDLYEFAKCGTYPTSLAFSQDGKKMATIAVDRKVRIFRFLTGKLMRVFDESLTMFTELQQMRQQLPDMEFGRRMAVERELEKVDGIRLTNIIFDETGHFVLYGTMLGIKVINVETNRCVRILGKQENIRVVQLSLFQGVAKAMQAAPTIEMKASENPALQSSDPDPTIFCTAFKKNRFYMFTKREPEDTKSADSDRDIFNEKPSKEEVMAATQAEGPKRVSDSAIIHTTMGDIHIKLFPVECPKTVENFCVHSRNGYYNGHIFHRVIKGFMIQTGDPTGTGMGGESIWGGEFEDEFHSTLRHDRPYTLSMANAGPGTNGSQFFITVVPTVRP

Target ORF information:

RefSeq Version	XM_021479088.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio peptidylprolyl isomerase domain and WD repeat containing 1 (ppwd1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479088.1

ORF Insert Sequence:

ATGGCGACCG ACGTGAGCAG TGATGGTTCT GAGAATAAAC GAAAACTGGA AGAAGATGAA 
GACGAAGACA ATTGGGTTGG TCCTATGCCG AGTGAGGCCA CACAAACCAA AAAGAAAAAA 
GTTCTTGAGT TTGAGAGATT GTACCTGGAT AATTTACCAT CTGCCTCCAT GTATGAAAGA 
AGTTACATGC ACAGAGATGT TATAACACAC ATTGTTTGCT CCAAAACTGA TTTTATCCTC 
ACAGCAAGTC AAGATGGACA TGTCAAATTC TGGAAGAAGA AAGAGGATGA AGGAGTGGAG 
TTTGTCAAGC ACTTCCGCAG CCACTTGGGA GTGATTGAGT GCATTTCCGT GAGTGCGGAG 
GGAGCACTTT TATGCTCTGT TGGTGATGAT CAAGCGATGA AAGTTTTTGA TGTAGTCAAC 
TTTGACATGA TCAACATGCT CAAACTTGGC TTCCATCCAG GCCAGTGTGA GTGGATTTAT 
AATCCAGGAG ACGCCATTTC AACAATCGCC TGCTCTGAGA AATCCACTGG AAAGATTTTT 
GTGTATGATG GACGTGGCAG TAATCAGCCC CTGCACGTAT TCGAAAAGAT GCACACCACA 
CCACTCTCTC AGATCCGCCT CAATCCCAAA TATCGTGTCA TCGTATCTGC CGATAAAGCG 
GGAATGCTAG AATACTGGAC GGGGCTCCCG AGCGAATTCA AATTTCCGCG GCAGGTGGAC 
TGGGAGTTTA AGACCGACAC AGACTTGTAT GAGTTTGCCA AATGTGGAAC GTATCCCACC 
AGCCTGGCTT TCTCTCAAGA TGGCAAGAAA ATGGCCACCA TTGCCGTTGA CCGCAAAGTG 
AGGATTTTCC GCTTTCTCAC TGGGAAGTTG ATGCGGGTGT TTGACGAATC ACTAACAATG 
TTTACAGAGC TCCAGCAGAT GAGACAGCAG TTGCCTGATA TGGAGTTTGG TCGCAGAATG 
GCAGTGGAGA GAGAGCTGGA GAAGGTGGAC GGCATACGAT TGACCAACAT CATCTTTGAT 
GAGACTGGAC ACTTTGTGCT TTACGGCACC ATGCTAGGCA TTAAAGTCAT CAACGTAGAG 
ACTAACAGGT GTGTGCGCAT ACTGGGCAAG CAGGAGAACA TCCGTGTGGT GCAGTTGAGT 
CTGTTTCAGG GAGTCGCAAA GGCCATGCAA GCGGCACCAA CAATTGAGAT GAAAGCCTCT 
GAAAACCCAG CACTCCAGAG CTCTGACCCA GACCCCACCA TCTTCTGCAC TGCCTTTAAG 
AAGAACCGCT TTTACATGTT TACAAAAAGA GAGCCCGAGG ACACAAAGAG TGCTGATTCT 
GACCGTGATA TTTTCAACGA GAAGCCGTCT AAAGAGGAAG TGATGGCAGC CACACAGGCG 
GAAGGACCAA AAAGAGTGTC AGACAGTGCC ATCATTCACA CCACTATGGG TGACATTCAC 
ATCAAACTCT TTCCTGTCGA ATGCCCCAAA ACTGTGGAGA ACTTCTGTGT TCACAGCAGG 
AACGGTTATT ACAACGGCCA CATTTTTCAC CGAGTCATCA AGGGTTTCAT GATCCAGACT 
GGAGACCCCA CAGGGACTGG CATGGGTGGG GAAAGCATCT GGGGTGGAGA GTTTGAGGAT 
GAGTTTCATT CCACTCTGAG ACATGACAGA CCCTACACCC TCAGCATGGC CAATGCAGGA 
CCAGGAACCA ACGGCTCACA GTTCTTCATC ACTGTCGTCC CAACCGTAAG ACCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Many genes in fish have species-specific asymmetric rates of molecular evolution.
BMC genomics720(2006 Feb)
Steinke D,Salzburger W,Braasch I,Meyer A

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ppwd1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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