cep135 cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow)

Gene
Clones
gRNAs

Summary

Gene Symbol	cep135
Entrez Gene ID	107091575
Full Name	centrosomal protein 135
Gene Type	protein-coding
Organism	Cyprinodon variegatus(sheepshead minnow)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015385578.1	XP_015241064.1	centrosomal protein of 135 kDa isoform X2
XM_015385577.1	XP_015241063.1	centrosomal protein of 135 kDa isoform X1

Pathways from BioSystems
Gene Ontology

The following cep135 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cep135 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCy16215	XM_015385577.1 Latest version!	Cyprinodon variegatus centrosomal protein 135kDa (cep135), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCy16216	XM_015385578.1 Latest version!	Cyprinodon variegatus centrosomal protein 135kDa (cep135), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cep135 ( XM_015385577.1 ) cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow) -> XP_015241063.1 Cyprinodon variegatus centrosomal protein 135kDa (cep135), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCy16215
Clone ID Related Accession (Same CDS sequence)	XM_015385577.1
Accession Version	XM_015385577.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-13
Organism	Cyprinodon variegatus(sheepshead minnow)
Product	centrosomal protein of 135 kDa isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015150796.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cyprinodon variegatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAGCA GCGCAGAGAG GAAATTCGTG AACCTTCGGA AACGTCTGGA TCAGCTGGGC 
TACAGACAAC CTTTAGGGAT TGAGTCGCTG CCGCTCGTGG AGAAACTGTT CAGCGACCTG 
CTTCACGTAA CCGAGAGCCT GCGCACTGCC AAACTATCAG CAGGAAAGAC CGAGAAAGAG 
AGCCGAAATG TTGATGCTCT GCTGGAGCCC TACAGGGCAG AGAATGCCAG GGTTGTCAGG 
GAAAACAATG ACCTGCATCT AGAGCTCTTG AAACTCAGGG AGGAGAAAGA TCGGGTCACT 
AGAGAACTCA AATCACAAAT CAGAAAACTG GATCATGAGA CATCTGATCT AAAGTTTCTA 
AACAACCAGT ATGTGCATAA AGTCCGCTGC TTGGAAAAGG ACAGCAAGGC TAAAGCTGAG 
CGGATCCAGC AGCTGCAGGA GAAGAACATG CAAGCTGTTG TGCAGACGCC AGGTGGGAAA 
AAGCGCAGTA TTCCTTTTCG ACGACAGAGA ATGCAGATTG ATGAGCTCCT ACCTCCTCCT 
TCTAAATCCA CCTATCCTGT CCCCCAGCCT AATGATCCAT ACATAGCTGA CCTGCTGCAG 
CTGGCCGATG GAAGGATTAA TGAACTACAG GACGACTTTA TCAAGCTTAA ACTGGACCTG 
GAAAATGGCC AAGAATGTAT AAAACATCTG AATACTCAGG TGGATGAAAG GGACAGAGAG 
ATAGAGCGTT TGAATCATGT CCTACAAGGA GGACGGCCTC ATGATGTCAT TTCTCTCGAA 
GCGCAGAACA TCAGCAATGA AAAACTGATC GCCCATCTCA ACCTTCAGAT CGAGTACCTG 
CAGGAGACCA ATAGGACCCT GGAGCAAAGG GTAGAAGGAC TGCAGCTAAA GAAGAAGGAC 
GCCTCCACAG AGGTGGCAAA TCTCTCCTTG AAGAACCTGG AGCTCTGTGA GGAACTGACA 
CATATAGATC ATCTTGCAAA ATGTATGGAG ATGGACAAGG AACGTGTGTT GGAAACTGCT 
GATATGGAAC TACAAGAGTC AAAAAAAGAA ATTCAAAGGC AGCAGAAAAC CATTGAAGAC 
TTGGAGGATA TTATCACAAA TTCTAGAAGG GAGCTGTCTG AAGGAGAATT TGAAAGAGAT 
CACTTAAGAG ATCAACTGAT GGAGCTGAAG GAGCAGAACA TGAAGATGGA GGGACTAGTG 
AACTTCCTGG AAGACGAGAA AACCAGGCTG CAGGACAAAG TGGAGAAGAT GATGGCCGCT 
GATAAAGAGC TGGTGCTGGA GTTGGAGGCT ATGCGTGTTA AACATGGTGT CTGTGGGAGA 
GAGCATTCCC CATCTCGTCT GGATGCCTTT GTCAAAAACC TAGAAGAGGA GAGGGATTTC 
TATCGCCACG AAGCTGAGCG GTATAAGAGA GCCAGAGGAG CCGGGGGACC AGGCGTGAGC 
CCCCGACGCA GTCCAGAGAG AAGCAGAAGT TTGAAAGGCA ATGTGACCAA AGGGAGTGTT 
TCAGAGGTAG AACTGATCCG CGTCATCAAA GAACGGGATG AGATCAAGGC CGCTCTGGTG 
GACTTTGAGA AGCACATGGA GGAAATCCAA AAGAATGTGA AAGGCCTCAC TGATGAGAGG 
GACCATTTCA AAACCCGCCT TAAGCAAGCT GAAGATGACC TAAAGTTGGC CCACAGAGCA 
GGTTTGTCGG CTGACATGTT GAGACTACAG GAGGAATTGA GACGTGCTGA GGTAAAGCTT 
GAGCAGATGA CCTTGGAAAG GGACACACTG ATGGAGAGGT TAAAGGTCGC CCAAGGTTCA 
GCTCTCAGTG ACAGAGAGGA GAGGAGGATG CTCGACTTGG AGAATACTGT CAAGAGTCTG 
GAGCAGGAGA GATTGGATCT GCAGTCTCAA GTGTTTCTGT TAAAGGAGAG TAAAGAGGCT 
GTGGAGGAAG AGCTGAAGGT TCGATCCACT GCTTCAGTCC AAAACGCAGA AGTTGTTGCC 
CAGCAGACGG CTGAGGCGAG CACTCTAAGG CTGCTGCAGG AGCAGATGGA GCAGTCACTG 
TCTGATATGC AGCGTAGACT TTCAGTGAAG ATGAATGAGC TGCATGTGGC CCATGAGCAG 
ATTGAAAAGC TGGAGGAGAG ATTAGGGGAG TTGAGTCAGC AGAGTTTAAA ACACAAAGAG 
GAAGTGGCTG CTCTCCAGAA GTCTATCTCT CTCCTGGATA GAGAGAAAGA CGCCCTGCAG 
GATGAGGTGG ATCAAAAGGC AGAAAAACTG GTTGCTCTCC AGGACAACCT GTCCCAGAAG 
GAAAAAACTC TTGACGATGT GAGACTCACT GTCAAAAATA TGGACAATTC CCTTGCTCAA 
TTGCAAGGAG CATTAAAGAT CCGTGAACGG GAAATCAGCA GCCTGAGGAG GCAGCTGGAT 
GCGTCTCAGG AGGAGCTGGC CGCCCTCAAG AGAGACAAAG AAGTCACGCT AAGAGAAAAC 
AGGAGGCTGC AGGATGACCT GGCCACCATG ACCAGGGAGA ACCAAGCGGT ACATTTAGAA 
ATGGAGGAGG CTTTGCATGA GAAGGATGAG TTAAAGTTGA GGGTCCACTC ATATATTTCG 
GAAGTATCCA GAATAGAGAA ACTAATGGCC ACAAAGGAGC AGGAGAACAG GGATTTACTG 
GAGCGCTTCA GGATGGCCCA CTCTGGCATG CAGGAAAAGG AGCAGAAGCT GCACCAGGCT 
GAATGCCTGA ATAACTCCAC CCGCCTCGAG CTGCTCTCCT CGGACACAGA GCGCAGGCAG 
CTCCGGGACA CACTAAGGCA AAAAGAGCGA GAGATCCAAC AGCACATGCA GGCCCTACAG 
GCCTATGAAG CCCAGGTGTC CTCACTGGTT CGTGGGATGT CCCGACTAGA AGAGGAGCTG 
AACAAAGCAC AGGAGGAGAA GGCCGCTCTC CTTTCAGATC TAGCCTCTGT CAGGGAGCTC 
TGTGTCAAAC TAGACTCCGG GAAGGACCTT GTTACACGAC AGCTGACCTC CAAGAGCATG 
GACTTAGAGA GGGTGAGTGG GGAACTGGAA GACGTTCGCT CAGAGGTTGA GGTCCTGAAG 
AAACAACTAG CCAGTGAGAG GCTGACCGTG CGCAACCTTG AGACACTGCT GTCTACCAAC 
CGGCAGAAGG AGTTCCAGAC TCATCTGACT GCCAGTGAGA GAGAGTCTGA ACTGAAAGTC 
CTGCGTGACA GGCTCACTGT GGCCGACAGC AAAAATGCAG AGCACGCCAG AGAGGTGTCC 
CAGCTCCGTG GAAAAGTGTC TCAACTGCAG ACAGAAATGG ATGTGCTGAA ACGACAGCTG 
ACCGCTGAGC GATTCGAACG AGAAAGAGCG GTTCAGGAGA TGCGCAGACA GGGATTGTCA 
TTTTCATCTT TGCAAAGCTC GTCTCCTCTC AGTTTATCTG GAGGCTCTCA TCACACCTCT 
GCAGAACGCT CCATCCTCCG AACTCAAGAG CACTCCTCAG ACAAATCTAC AGACAAGTCT 
ACAGACAAGA GCGTTAGCTT CAAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015241063.1
CDS	416..3925
Translation	MNSSAERKFVNLRKRLDQLGYRQPLGIESLPLVEKLFSDLLHVTESLRTAKLSAGKTEKESRNVDALLEPYRAENARVVRENNDLHLELLKLREEKDRVTRELKSQIRKLDHETSDLKFLNNQYVHKVRCLEKDSKAKAERIQQLQEKNMQAVVQTPGGKKRSIPFRRQRMQIDELLPPPSKSTYPVPQPNDPYIADLLQLADGRINELQDDFIKLKLDLENGQECIKHLNTQVDERDREIERLNHVLQGGRPHDVISLEAQNISNEKLIAHLNLQIEYLQETNRTLEQRVEGLQLKKKDASTEVANLSLKNLELCEELTHIDHLAKCMEMDKERVLETADMELQESKKEIQRQQKTIEDLEDIITNSRRELSEGEFERDHLRDQLMELKEQNMKMEGLVNFLEDEKTRLQDKVEKMMAADKELVLELEAMRVKHGVCGREHSPSRLDAFVKNLEEERDFYRHEAERYKRARGAGGPGVSPRRSPERSRSLKGNVTKGSVSEVELIRVIKERDEIKAALVDFEKHMEEIQKNVKGLTDERDHFKTRLKQAEDDLKLAHRAGLSADMLRLQEELRRAEVKLEQMTLERDTLMERLKVAQGSALSDREERRMLDLENTVKSLEQERLDLQSQVFLLKESKEAVEEELKVRSTASVQNAEVVAQQTAEASTLRLLQEQMEQSLSDMQRRLSVKMNELHVAHEQIEKLEERLGELSQQSLKHKEEVAALQKSISLLDREKDALQDEVDQKAEKLVALQDNLSQKEKTLDDVRLTVKNMDNSLAQLQGALKIREREISSLRRQLDASQEELAALKRDKEVTLRENRRLQDDLATMTRENQAVHLEMEEALHEKDELKLRVHSYISEVSRIEKLMATKEQENRDLLERFRMAHSGMQEKEQKLHQAECLNNSTRLELLSSDTERRQLRDTLRQKEREIQQHMQALQAYEAQVSSLVRGMSRLEEELNKAQEEKAALLSDLASVRELCVKLDSGKDLVTRQLTSKSMDLERVSGELEDVRSEVEVLKKQLASERLTVRNLETLLSTNRQKEFQTHLTASERESELKVLRDRLTVADSKNAEHAREVSQLRGKVSQLQTEMDVLKRQLTAERFERERAVQEMRRQGLSFSSLQSSSPLSLSGGSHHTSAERSILRTQEHSSDKSTDKSTDKSVSFKD

Target ORF information:

RefSeq Version	XM_015385577.1
Organism	Cyprinodon variegatus(sheepshead minnow)
Definition	Cyprinodon variegatus centrosomal protein 135kDa (cep135), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015385577.1

ORF Insert Sequence:

ATGAACAGCA GCGCAGAGAG GAAATTCGTG AACCTTCGGA AACGTCTGGA TCAGCTGGGC 
TACAGACAAC CTTTAGGGAT TGAGTCGCTG CCGCTCGTGG AGAAACTGTT CAGCGACCTG 
CTTCACGTAA CCGAGAGCCT GCGCACTGCC AAACTATCAG CAGGAAAGAC CGAGAAAGAG 
AGCCGAAATG TTGATGCTCT GCTGGAGCCC TACAGGGCAG AGAATGCCAG GGTTGTCAGG 
GAAAACAATG ACCTGCATCT AGAGCTCTTG AAACTCAGGG AGGAGAAAGA TCGGGTCACT 
AGAGAACTCA AATCACAAAT CAGAAAACTG GATCATGAGA CATCTGATCT AAAGTTTCTA 
AACAACCAGT ATGTGCATAA AGTCCGCTGC TTGGAAAAGG ACAGCAAGGC TAAAGCTGAG 
CGGATCCAGC AGCTGCAGGA GAAGAACATG CAAGCTGTTG TGCAGACGCC AGGTGGGAAA 
AAGCGCAGTA TTCCTTTTCG ACGACAGAGA ATGCAGATTG ATGAGCTCCT ACCTCCTCCT 
TCTAAATCCA CCTATCCTGT CCCCCAGCCT AATGATCCAT ACATAGCTGA CCTGCTGCAG 
CTGGCCGATG GAAGGATTAA TGAACTACAG GACGACTTTA TCAAGCTTAA ACTGGACCTG 
GAAAATGGCC AAGAATGTAT AAAACATCTG AATACTCAGG TGGATGAAAG GGACAGAGAG 
ATAGAGCGTT TGAATCATGT CCTACAAGGA GGACGGCCTC ATGATGTCAT TTCTCTCGAA 
GCGCAGAACA TCAGCAATGA AAAACTGATC GCCCATCTCA ACCTTCAGAT CGAGTACCTG 
CAGGAGACCA ATAGGACCCT GGAGCAAAGG GTAGAAGGAC TGCAGCTAAA GAAGAAGGAC 
GCCTCCACAG AGGTGGCAAA TCTCTCCTTG AAGAACCTGG AGCTCTGTGA GGAACTGACA 
CATATAGATC ATCTTGCAAA ATGTATGGAG ATGGACAAGG AACGTGTGTT GGAAACTGCT 
GATATGGAAC TACAAGAGTC AAAAAAAGAA ATTCAAAGGC AGCAGAAAAC CATTGAAGAC 
TTGGAGGATA TTATCACAAA TTCTAGAAGG GAGCTGTCTG AAGGAGAATT TGAAAGAGAT 
CACTTAAGAG ATCAACTGAT GGAGCTGAAG GAGCAGAACA TGAAGATGGA GGGACTAGTG 
AACTTCCTGG AAGACGAGAA AACCAGGCTG CAGGACAAAG TGGAGAAGAT GATGGCCGCT 
GATAAAGAGC TGGTGCTGGA GTTGGAGGCT ATGCGTGTTA AACATGGTGT CTGTGGGAGA 
GAGCATTCCC CATCTCGTCT GGATGCCTTT GTCAAAAACC TAGAAGAGGA GAGGGATTTC 
TATCGCCACG AAGCTGAGCG GTATAAGAGA GCCAGAGGAG CCGGGGGACC AGGCGTGAGC 
CCCCGACGCA GTCCAGAGAG AAGCAGAAGT TTGAAAGGCA ATGTGACCAA AGGGAGTGTT 
TCAGAGGTAG AACTGATCCG CGTCATCAAA GAACGGGATG AGATCAAGGC CGCTCTGGTG 
GACTTTGAGA AGCACATGGA GGAAATCCAA AAGAATGTGA AAGGCCTCAC TGATGAGAGG 
GACCATTTCA AAACCCGCCT TAAGCAAGCT GAAGATGACC TAAAGTTGGC CCACAGAGCA 
GGTTTGTCGG CTGACATGTT GAGACTACAG GAGGAATTGA GACGTGCTGA GGTAAAGCTT 
GAGCAGATGA CCTTGGAAAG GGACACACTG ATGGAGAGGT TAAAGGTCGC CCAAGGTTCA 
GCTCTCAGTG ACAGAGAGGA GAGGAGGATG CTCGACTTGG AGAATACTGT CAAGAGTCTG 
GAGCAGGAGA GATTGGATCT GCAGTCTCAA GTGTTTCTGT TAAAGGAGAG TAAAGAGGCT 
GTGGAGGAAG AGCTGAAGGT TCGATCCACT GCTTCAGTCC AAAACGCAGA AGTTGTTGCC 
CAGCAGACGG CTGAGGCGAG CACTCTAAGG CTGCTGCAGG AGCAGATGGA GCAGTCACTG 
TCTGATATGC AGCGTAGACT TTCAGTGAAG ATGAATGAGC TGCATGTGGC CCATGAGCAG 
ATTGAAAAGC TGGAGGAGAG ATTAGGGGAG TTGAGTCAGC AGAGTTTAAA ACACAAAGAG 
GAAGTGGCTG CTCTCCAGAA GTCTATCTCT CTCCTGGATA GAGAGAAAGA CGCCCTGCAG 
GATGAGGTGG ATCAAAAGGC AGAAAAACTG GTTGCTCTCC AGGACAACCT GTCCCAGAAG 
GAAAAAACTC TTGACGATGT GAGACTCACT GTCAAAAATA TGGACAATTC CCTTGCTCAA 
TTGCAAGGAG CATTAAAGAT CCGTGAACGG GAAATCAGCA GCCTGAGGAG GCAGCTGGAT 
GCGTCTCAGG AGGAGCTGGC CGCCCTCAAG AGAGACAAAG AAGTCACGCT AAGAGAAAAC 
AGGAGGCTGC AGGATGACCT GGCCACCATG ACCAGGGAGA ACCAAGCGGT ACATTTAGAA 
ATGGAGGAGG CTTTGCATGA GAAGGATGAG TTAAAGTTGA GGGTCCACTC ATATATTTCG 
GAAGTATCCA GAATAGAGAA ACTAATGGCC ACAAAGGAGC AGGAGAACAG GGATTTACTG 
GAGCGCTTCA GGATGGCCCA CTCTGGCATG CAGGAAAAGG AGCAGAAGCT GCACCAGGCT 
GAATGCCTGA ATAACTCCAC CCGCCTCGAG CTGCTCTCCT CGGACACAGA GCGCAGGCAG 
CTCCGGGACA CACTAAGGCA AAAAGAGCGA GAGATCCAAC AGCACATGCA GGCCCTACAG 
GCCTATGAAG CCCAGGTGTC CTCACTGGTT CGTGGGATGT CCCGACTAGA AGAGGAGCTG 
AACAAAGCAC AGGAGGAGAA GGCCGCTCTC CTTTCAGATC TAGCCTCTGT CAGGGAGCTC 
TGTGTCAAAC TAGACTCCGG GAAGGACCTT GTTACACGAC AGCTGACCTC CAAGAGCATG 
GACTTAGAGA GGGTGAGTGG GGAACTGGAA GACGTTCGCT CAGAGGTTGA GGTCCTGAAG 
AAACAACTAG CCAGTGAGAG GCTGACCGTG CGCAACCTTG AGACACTGCT GTCTACCAAC 
CGGCAGAAGG AGTTCCAGAC TCATCTGACT GCCAGTGAGA GAGAGTCTGA ACTGAAAGTC 
CTGCGTGACA GGCTCACTGT GGCCGACAGC AAAAATGCAG AGCACGCCAG AGAGGTGTCC 
CAGCTCCGTG GAAAAGTGTC TCAACTGCAG ACAGAAATGG ATGTGCTGAA ACGACAGCTG 
ACCGCTGAGC GATTCGAACG AGAAAGAGCG GTTCAGGAGA TGCGCAGACA GGGATTGTCA 
TTTTCATCTT TGCAAAGCTC GTCTCCTCTC AGTTTATCTG GAGGCTCTCA TCACACCTCT 
GCAGAACGCT CCATCCTCCG AACTCAAGAG CACTCCTCAG ACAAATCTAC AGACAAGTCT 
ACAGACAAGA GCGTTAGCTT CAAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cep135 ( XM_015385578.1 ) cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow) -> XP_015241064.1 Cyprinodon variegatus centrosomal protein 135kDa (cep135), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCy16216
Clone ID Related Accession (Same CDS sequence)	XM_015385578.1
Accession Version	XM_015385578.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-13
Organism	Cyprinodon variegatus(sheepshead minnow)
Product	centrosomal protein of 135 kDa isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015150796.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cyprinodon variegatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAGCA GCGCAGAGAG GAAATTCGTG AACCTTCGGA AACGTCTGGA TCAGCTGGGC 
TACAGACAAC CTTTAGGGAT TGAGTCGCTG CCGCTCGTGG AGAAACTGTT CAGCGACCTG 
CTTCACGTAA CCGAGAGCCT GCGCACTGCC AAACTATCAG CAGGAAAGAC CGAGAAAGAG 
AGCCGAAATG TTGATGCTCT GCTGGAGCCC TACAGGGCAG AGAATGCCAG GGTTGTCAGG 
GAAAACAATG ACCTGCATCT AGAGCTCTTG AAACTCAGGG AGGAGAAAGA TCGGGTCACT 
AGAGAACTCA AATCACAAAT CAGAAAACTG GATCATGAGA CATCTGATCT AAAGTTTCTA 
AACAACCAGT ATGTGCATAA AGTCCGCTGC TTGGAAAAGG ACAGCAAGGC TAAAGCTGAG 
CGGATCCAGC AGCTGCAGGA GAAGAACATG CAAGCTGTTG TGCAGACGCC AGGTGGGAAA 
AAGCGCAGTA TTCCTTTTCG ACGACAGAGA ATGCAGATTG ATGAGCTCCT ACCTCCTCCT 
TCTAAATCCA CCTATCCTGT CCCCCAGCCT AATGATCCAT ACATAGCTGA CCTGCTGCAG 
CTGGCCGATG GAAGGATTAA TGAACTACAG GACGACTTTA TCAAGCTTAA ACTGGACCTG 
GAAAATGGCC AAGAATGTAT AAAACATCTG AATACTCAGG TGGATGAAAG GGACAGAGAG 
ATAGAGCGTT TGAATCATGT CCTACAAGGA GGACGGCCTC ATGATGTCAT TTCTCTCGAA 
GCGCAGAACA TCAGCAATGA AAAACTGATC GCCCATCTCA ACCTTCAGAT CGAGTACCTG 
CAGGAGACCA ATAGGACCCT GGAGCAAAGG GTAGAAGGAC TGCAGCTAAA GAAGAAGGAC 
GCCTCCACAG AGGTGGCAAA TCTCTCCTTG AAGAACCTGG AGCTCTGTGA GGAACTGACA 
CATATAGATC ATCTTGCAAA ATGTATGGAG ATGGACAAGG AACGTGTGTT GGAAACTGCT 
GATATGGAAC TACAAGAGTC AAAAAAAGAA ATTCAAAGGC AGCAGAAAAC CATTGAAGAC 
TTGGAGGATA TTATCACAAA TTCTAGAAGG GAGCTGTCTG AAGGAGAATT TGAAAGAGAT 
CACTTAAGAG ATCAACTGAT GGAGCTGAAG GAGCAGAACA TGAAGATGGA GGGACTAGTG 
AACTTCCTGG AAGACGAGAA AACCAGGCTG CAGGACAAAG TGGAGAAGAT GATGGCCGCT 
GATAAAGAGC TGGTGCTGGA GTTGGAGGCT ATGCGTGTTA AACATGGTGT CTGTGGGAGA 
GAGCATTCCC CATCTCGTCT GGATGCCTTT GTCAAAAACC TAGAAGAGGA GAGGGATTTC 
TATCGCCACG AAGCTGAGCG GTATAAGAGA GCCAGAGGAG CCGGGGGACC AGGCGTGAGC 
CCCCGACGCA GTCCAGAGAG AAGCAGAAGT TTGAAAGGCA ATGTGACCAA AGGGAGTGTT 
TCAGAGGTAG AACTGATCCG CGTCATCAAA GAACGGGATG AGATCAAGGC CGCTCTGGTG 
GACTTTGAGA AGCACATGGA GGAAATCCAA AAGAATGTGA AAGGCCTCAC TGATGAGAGG 
GACCATTTCA AAACCCGCCT TAAGCAAGCT GAAGATGACC TAAAGTTGGC CCACAGAGCA 
GGTTTGTCGG CTGACATGTT GAGACTACAG GAGGAATTGA GACGTGCTGA GGTAAAGCTT 
GAGCAGATGA CCTTGGAAAG GGACACACTG ATGGAGAGGT TAAAGGTCGC CCAAGGTTCA 
GCTCTCAGTG ACAGAGAGGA GAGGAGGATG CTCGACTTGG AGAATACTGT CAAGAGTCTG 
GAGCAGGAGA GATTGGATCT GCAGTCTCAA GTGTTTCTGT TAAAGGAGAG TAAAGAGGCT 
GTGGAGGAAG AGCTGAAGGT TCGATCCACT GCTTCAGTCC AAAACGCAGA AGTTGTTGCC 
CAGCAGACGG CTGAGGCGAG CACTCTAAGG CTGCTGCAGG AGCAGATGGA GCAGTCACTG 
TCTGATATGC AGCGTAGACT TTCAGTGAAG ATGAATGAGC TGCATGTGGC CCATGAGCAG 
ATTGAAAAGC TGGAGGAGAG ATTAGGGGAG TTGAGTCAGC AGAGTTTAAA ACACAAAGAG 
GAAGTGGCTG CTCTCCAGAA GTCTATCTCT CTCCTGGATA GAGAGAAAGA CGCCCTGCAG 
GATGAGGTGG ATCAAAAGGC AGAAAAACTG GTTGCTCTCC AGGACAACCT GTCCCAGAAG 
GAAAAAACTC TTGACGATGT GAGACTCACT GTCAAAAATA TGGACAATTC CCTTGCTCAA 
TTGCAAGGAG CATTAAAGAT CCGTGAACGG GAAATCAGCA GCCTGAGGAG GCAGCTGGAT 
GCGTCTCAGG AGGAGCTGGC CGCCCTCAAG AGAGACAAAG AAGTCACGCT AAGAGAAAAC 
AGGAGGCTGC AGGATGACCT GGCCACCATG ACCAGGGAGA ACCAAGCGGT ACATTTAGAA 
ATGGAGGAGG CTTTGCATGA GAAGGATGAG TTAAAGTTGA GGGTCCACTC ATATATTTCG 
GAAGTATCCA GAATAGAGAA ACTAATGGCC ACAAAGGAGC AGGAGAACAG GGATTTACTG 
GAGCGCTTCA GGATGGCCCA CTCTGGCATG CAGGAAAAGG AGCAGAAGCT GCACCAGGCT 
GAATGCCTGA ATAACTCCAC CCGCCTCGAG CTGCTCTCCT CGGACACAGA GCGCAGGCAG 
CTCCGGGACA CACTAAGGCA AAAAGAGCGA GAGATCCAAC AGCACATGCA GGCCCTACAG 
GCCTATGAAG CCCAGGTGTC CTCACTGGTT CGTGGGATGT CCCGACTAGA AGAGGAGCTG 
AACAAAGCAC AGGAGGAGAA GGCCGCTCTC CTTTCAGATC TAGCCTCTGT CAGGGAGCTC 
TGTGTCAAAC TAGACTCCGG GAAGGACCTT GTTACACGAC AGCTGACCTC CAAGAGCATG 
GACTTAGAGA GGGTGAGTGG GGAACTGGAA GACGTTCGCT CAGAGGTTGA GGTCCTGAAG 
AAACAACTAG CCAGTGAGAG GCTGACCGTG CGCAACCTTG AGACACTGCT GTCTACCAAC 
CGGCAGAAGG AGTTCCAGAC TCATCTGACT GCCAGTGAGA GAGAGTCTGA ACTGAAAGTC 
CTGCGTGACA GGCTCACTGT GGCCGACAGC AAAAATGCAG AGCACGCCAG AGAGGTGTCC 
CAGCTCCGTG GAAAAGTGTC TCAACTGCAG ACAGAAATGG ATGTGCTGAA ACGACAGCTG 
ACCGCTGAGC GATTCGAACG AGAAAGAGCG GTTCAGGAGA TGCGCAGACA GGGATTGTCA 
TTTTCATCTT TGCAAAGCTC GTCTCCTCTC AGTTTATCTG GAGGCTCTCA TCACACCTCT 
GCAGAACGCT CCATCCTCCG AACTCAAGAG CACTCCTCAG ACAAATCTAC AGACAAGAGC 
GTTAGCTTCA AGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015241064.1
CDS	416..3913
Translation	MNSSAERKFVNLRKRLDQLGYRQPLGIESLPLVEKLFSDLLHVTESLRTAKLSAGKTEKESRNVDALLEPYRAENARVVRENNDLHLELLKLREEKDRVTRELKSQIRKLDHETSDLKFLNNQYVHKVRCLEKDSKAKAERIQQLQEKNMQAVVQTPGGKKRSIPFRRQRMQIDELLPPPSKSTYPVPQPNDPYIADLLQLADGRINELQDDFIKLKLDLENGQECIKHLNTQVDERDREIERLNHVLQGGRPHDVISLEAQNISNEKLIAHLNLQIEYLQETNRTLEQRVEGLQLKKKDASTEVANLSLKNLELCEELTHIDHLAKCMEMDKERVLETADMELQESKKEIQRQQKTIEDLEDIITNSRRELSEGEFERDHLRDQLMELKEQNMKMEGLVNFLEDEKTRLQDKVEKMMAADKELVLELEAMRVKHGVCGREHSPSRLDAFVKNLEEERDFYRHEAERYKRARGAGGPGVSPRRSPERSRSLKGNVTKGSVSEVELIRVIKERDEIKAALVDFEKHMEEIQKNVKGLTDERDHFKTRLKQAEDDLKLAHRAGLSADMLRLQEELRRAEVKLEQMTLERDTLMERLKVAQGSALSDREERRMLDLENTVKSLEQERLDLQSQVFLLKESKEAVEEELKVRSTASVQNAEVVAQQTAEASTLRLLQEQMEQSLSDMQRRLSVKMNELHVAHEQIEKLEERLGELSQQSLKHKEEVAALQKSISLLDREKDALQDEVDQKAEKLVALQDNLSQKEKTLDDVRLTVKNMDNSLAQLQGALKIREREISSLRRQLDASQEELAALKRDKEVTLRENRRLQDDLATMTRENQAVHLEMEEALHEKDELKLRVHSYISEVSRIEKLMATKEQENRDLLERFRMAHSGMQEKEQKLHQAECLNNSTRLELLSSDTERRQLRDTLRQKEREIQQHMQALQAYEAQVSSLVRGMSRLEEELNKAQEEKAALLSDLASVRELCVKLDSGKDLVTRQLTSKSMDLERVSGELEDVRSEVEVLKKQLASERLTVRNLETLLSTNRQKEFQTHLTASERESELKVLRDRLTVADSKNAEHAREVSQLRGKVSQLQTEMDVLKRQLTAERFERERAVQEMRRQGLSFSSLQSSSPLSLSGGSHHTSAERSILRTQEHSSDKSTDKSVSFKD

Target ORF information:

RefSeq Version	XM_015385578.1
Organism	Cyprinodon variegatus(sheepshead minnow)
Definition	Cyprinodon variegatus centrosomal protein 135kDa (cep135), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015385578.1

ORF Insert Sequence:

ATGAACAGCA GCGCAGAGAG GAAATTCGTG AACCTTCGGA AACGTCTGGA TCAGCTGGGC 
TACAGACAAC CTTTAGGGAT TGAGTCGCTG CCGCTCGTGG AGAAACTGTT CAGCGACCTG 
CTTCACGTAA CCGAGAGCCT GCGCACTGCC AAACTATCAG CAGGAAAGAC CGAGAAAGAG 
AGCCGAAATG TTGATGCTCT GCTGGAGCCC TACAGGGCAG AGAATGCCAG GGTTGTCAGG 
GAAAACAATG ACCTGCATCT AGAGCTCTTG AAACTCAGGG AGGAGAAAGA TCGGGTCACT 
AGAGAACTCA AATCACAAAT CAGAAAACTG GATCATGAGA CATCTGATCT AAAGTTTCTA 
AACAACCAGT ATGTGCATAA AGTCCGCTGC TTGGAAAAGG ACAGCAAGGC TAAAGCTGAG 
CGGATCCAGC AGCTGCAGGA GAAGAACATG CAAGCTGTTG TGCAGACGCC AGGTGGGAAA 
AAGCGCAGTA TTCCTTTTCG ACGACAGAGA ATGCAGATTG ATGAGCTCCT ACCTCCTCCT 
TCTAAATCCA CCTATCCTGT CCCCCAGCCT AATGATCCAT ACATAGCTGA CCTGCTGCAG 
CTGGCCGATG GAAGGATTAA TGAACTACAG GACGACTTTA TCAAGCTTAA ACTGGACCTG 
GAAAATGGCC AAGAATGTAT AAAACATCTG AATACTCAGG TGGATGAAAG GGACAGAGAG 
ATAGAGCGTT TGAATCATGT CCTACAAGGA GGACGGCCTC ATGATGTCAT TTCTCTCGAA 
GCGCAGAACA TCAGCAATGA AAAACTGATC GCCCATCTCA ACCTTCAGAT CGAGTACCTG 
CAGGAGACCA ATAGGACCCT GGAGCAAAGG GTAGAAGGAC TGCAGCTAAA GAAGAAGGAC 
GCCTCCACAG AGGTGGCAAA TCTCTCCTTG AAGAACCTGG AGCTCTGTGA GGAACTGACA 
CATATAGATC ATCTTGCAAA ATGTATGGAG ATGGACAAGG AACGTGTGTT GGAAACTGCT 
GATATGGAAC TACAAGAGTC AAAAAAAGAA ATTCAAAGGC AGCAGAAAAC CATTGAAGAC 
TTGGAGGATA TTATCACAAA TTCTAGAAGG GAGCTGTCTG AAGGAGAATT TGAAAGAGAT 
CACTTAAGAG ATCAACTGAT GGAGCTGAAG GAGCAGAACA TGAAGATGGA GGGACTAGTG 
AACTTCCTGG AAGACGAGAA AACCAGGCTG CAGGACAAAG TGGAGAAGAT GATGGCCGCT 
GATAAAGAGC TGGTGCTGGA GTTGGAGGCT ATGCGTGTTA AACATGGTGT CTGTGGGAGA 
GAGCATTCCC CATCTCGTCT GGATGCCTTT GTCAAAAACC TAGAAGAGGA GAGGGATTTC 
TATCGCCACG AAGCTGAGCG GTATAAGAGA GCCAGAGGAG CCGGGGGACC AGGCGTGAGC 
CCCCGACGCA GTCCAGAGAG AAGCAGAAGT TTGAAAGGCA ATGTGACCAA AGGGAGTGTT 
TCAGAGGTAG AACTGATCCG CGTCATCAAA GAACGGGATG AGATCAAGGC CGCTCTGGTG 
GACTTTGAGA AGCACATGGA GGAAATCCAA AAGAATGTGA AAGGCCTCAC TGATGAGAGG 
GACCATTTCA AAACCCGCCT TAAGCAAGCT GAAGATGACC TAAAGTTGGC CCACAGAGCA 
GGTTTGTCGG CTGACATGTT GAGACTACAG GAGGAATTGA GACGTGCTGA GGTAAAGCTT 
GAGCAGATGA CCTTGGAAAG GGACACACTG ATGGAGAGGT TAAAGGTCGC CCAAGGTTCA 
GCTCTCAGTG ACAGAGAGGA GAGGAGGATG CTCGACTTGG AGAATACTGT CAAGAGTCTG 
GAGCAGGAGA GATTGGATCT GCAGTCTCAA GTGTTTCTGT TAAAGGAGAG TAAAGAGGCT 
GTGGAGGAAG AGCTGAAGGT TCGATCCACT GCTTCAGTCC AAAACGCAGA AGTTGTTGCC 
CAGCAGACGG CTGAGGCGAG CACTCTAAGG CTGCTGCAGG AGCAGATGGA GCAGTCACTG 
TCTGATATGC AGCGTAGACT TTCAGTGAAG ATGAATGAGC TGCATGTGGC CCATGAGCAG 
ATTGAAAAGC TGGAGGAGAG ATTAGGGGAG TTGAGTCAGC AGAGTTTAAA ACACAAAGAG 
GAAGTGGCTG CTCTCCAGAA GTCTATCTCT CTCCTGGATA GAGAGAAAGA CGCCCTGCAG 
GATGAGGTGG ATCAAAAGGC AGAAAAACTG GTTGCTCTCC AGGACAACCT GTCCCAGAAG 
GAAAAAACTC TTGACGATGT GAGACTCACT GTCAAAAATA TGGACAATTC CCTTGCTCAA 
TTGCAAGGAG CATTAAAGAT CCGTGAACGG GAAATCAGCA GCCTGAGGAG GCAGCTGGAT 
GCGTCTCAGG AGGAGCTGGC CGCCCTCAAG AGAGACAAAG AAGTCACGCT AAGAGAAAAC 
AGGAGGCTGC AGGATGACCT GGCCACCATG ACCAGGGAGA ACCAAGCGGT ACATTTAGAA 
ATGGAGGAGG CTTTGCATGA GAAGGATGAG TTAAAGTTGA GGGTCCACTC ATATATTTCG 
GAAGTATCCA GAATAGAGAA ACTAATGGCC ACAAAGGAGC AGGAGAACAG GGATTTACTG 
GAGCGCTTCA GGATGGCCCA CTCTGGCATG CAGGAAAAGG AGCAGAAGCT GCACCAGGCT 
GAATGCCTGA ATAACTCCAC CCGCCTCGAG CTGCTCTCCT CGGACACAGA GCGCAGGCAG 
CTCCGGGACA CACTAAGGCA AAAAGAGCGA GAGATCCAAC AGCACATGCA GGCCCTACAG 
GCCTATGAAG CCCAGGTGTC CTCACTGGTT CGTGGGATGT CCCGACTAGA AGAGGAGCTG 
AACAAAGCAC AGGAGGAGAA GGCCGCTCTC CTTTCAGATC TAGCCTCTGT CAGGGAGCTC 
TGTGTCAAAC TAGACTCCGG GAAGGACCTT GTTACACGAC AGCTGACCTC CAAGAGCATG 
GACTTAGAGA GGGTGAGTGG GGAACTGGAA GACGTTCGCT CAGAGGTTGA GGTCCTGAAG 
AAACAACTAG CCAGTGAGAG GCTGACCGTG CGCAACCTTG AGACACTGCT GTCTACCAAC 
CGGCAGAAGG AGTTCCAGAC TCATCTGACT GCCAGTGAGA GAGAGTCTGA ACTGAAAGTC 
CTGCGTGACA GGCTCACTGT GGCCGACAGC AAAAATGCAG AGCACGCCAG AGAGGTGTCC 
CAGCTCCGTG GAAAAGTGTC TCAACTGCAG ACAGAAATGG ATGTGCTGAA ACGACAGCTG 
ACCGCTGAGC GATTCGAACG AGAAAGAGCG GTTCAGGAGA TGCGCAGACA GGGATTGTCA 
TTTTCATCTT TGCAAAGCTC GTCTCCTCTC AGTTTATCTG GAGGCTCTCA TCACACCTCT 
GCAGAACGCT CCATCCTCCG AACTCAAGAG CACTCCTCAG ACAAATCTAC AGACAAGAGC 
GTTAGCTTCA AGGACTGA