nans cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow)

Gene
Clones
gRNAs

Summary

Gene Symbol	nans
Entrez Gene ID	107083396
Full Name	N-acetylneuraminate synthase
Gene Type	protein-coding
Organism	Cyprinodon variegatus(sheepshead minnow)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015372596.1	XP_015228082.1	sialic acid synthase

Pathways from BioSystems
Gene Ontology

The following nans gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nans cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCy05790	XM_015372596.1 Latest version!	Cyprinodon variegatus N-acetylneuraminate synthase (nans), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nans ( XM_015372596.1 ) cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow) -> XP_015228082.1 Cyprinodon variegatus N-acetylneuraminate synthase (nans), mRNA.

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CloneID	OCy05790
Clone ID Related Accession (Same CDS sequence)	XM_015372596.1
Accession Version	XM_015372596.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-13
Organism	Cyprinodon variegatus(sheepshead minnow)
Product	sialic acid synthase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015150527.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cyprinodon variegatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCTGG AGTTTGAGCT GTGCCCCGGC CGGATGATCG GAGGGAACCA TCCATGCTTC 
ATCATAGCTG AGATTGGACA GAACCACCAG GGAGACATCG AGATAGCCAA GAAAATGATT 
AAAATGGCAA AGGACTGCGG TGCTGATTGT GCCAAATTCC AGAAGAGTGA GCTGGAGTAC 
AAATTCAACA AGAGAGCCTT GGAGCGTCCA TACACCTCCA AACACTCCTG GGGAAAAACC 
TATGGTGAGC ACAAGCGCCA CTTGGAGTTC AACCACGATC AGTACAGGGA GCTGCAGAAA 
TACGCAGAGG AGGTGGGGAT CTTCTTCACA GCCTCAGGGA TGGACGAGAT GGCAGTGGAA 
TTTCTCCATG AGCTCAATGT GCCTTTTTTC AAAGTGGGAT CCGGAGACAC CAACAACTTT 
CCTTATCTGG AGAAAACAGC CAAGAAGGGC CGTCCCATGG TGGTGTCCAG CGGGATGCAG 
ACTATGGAGA CGATGCGTCA GGTCTACAAG ACTGTGAAAC AGCACAATAA AAACTTTGCC 
ATCCTTCAGT GCACCAGTGC GTATCCTCTG GAGGCTGAAG ATGTCAACCT CAAAGTGATC 
GCGGAATACC AGAAAGAGTT TCCTGATATT CCAATCGGGT ATTCAGGTCA CGAGTCTGGA 
ATCACTATTT CTGTGGGAGC CGTAGCTCTG GGAGCGAAGG TCCTGGAGCG TCACATAACC 
TTGGACAAGT TTTGGAAAGG AAGCGACCAT GAGGCCTCGT TGATACCGTC TGAACTGGCT 
GAGCTGGTCC GCTCTGTTCG TCTGATGGAG AGGGCACTGG GAAATGGCAT CAAGCAGATG 
TTGCCCTGTG AGAAGGCCTG CCATGACAAA CTTGGCAAAT CTGTGGTGGC GAAGGTGAAG 
ATCCCCAAAG GAACAGTCCT GACTCTGGAC ATGTTGGGGG TGAAGGTGGC TGAGCCGATG 
GGTGTCCCGG CAGAGGACAT CTTCCAGCTG GTTGGGAAGA CTGTGAAGGA GGAGGTGGAG 
GAGGATGAGA GTGTTCAGCC TGAAGTGGTG GACCGCTATG GCAAGAAGGC CAAGTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015228082.1
CDS	131..1210
Translation	MPLEFELCPGRMIGGNHPCFIIAEIGQNHQGDIEIAKKMIKMAKDCGADCAKFQKSELEYKFNKRALERPYTSKHSWGKTYGEHKRHLEFNHDQYRELQKYAEEVGIFFTASGMDEMAVEFLHELNVPFFKVGSGDTNNFPYLEKTAKKGRPMVVSSGMQTMETMRQVYKTVKQHNKNFAILQCTSAYPLEAEDVNLKVIAEYQKEFPDIPIGYSGHESGITISVGAVALGAKVLERHITLDKFWKGSDHEASLIPSELAELVRSVRLMERALGNGIKQMLPCEKACHDKLGKSVVAKVKIPKGTVLTLDMLGVKVAEPMGVPAEDIFQLVGKTVKEEVEEDESVQPEVVDRYGKKAKC

Target ORF information:

RefSeq Version	XM_015372596.1
Organism	Cyprinodon variegatus(sheepshead minnow)
Definition	Cyprinodon variegatus N-acetylneuraminate synthase (nans), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015372596.1

ORF Insert Sequence:

ATGCCTCTGG AGTTTGAGCT GTGCCCCGGC CGGATGATCG GAGGGAACCA TCCATGCTTC 
ATCATAGCTG AGATTGGACA GAACCACCAG GGAGACATCG AGATAGCCAA GAAAATGATT 
AAAATGGCAA AGGACTGCGG TGCTGATTGT GCCAAATTCC AGAAGAGTGA GCTGGAGTAC 
AAATTCAACA AGAGAGCCTT GGAGCGTCCA TACACCTCCA AACACTCCTG GGGAAAAACC 
TATGGTGAGC ACAAGCGCCA CTTGGAGTTC AACCACGATC AGTACAGGGA GCTGCAGAAA 
TACGCAGAGG AGGTGGGGAT CTTCTTCACA GCCTCAGGGA TGGACGAGAT GGCAGTGGAA 
TTTCTCCATG AGCTCAATGT GCCTTTTTTC AAAGTGGGAT CCGGAGACAC CAACAACTTT 
CCTTATCTGG AGAAAACAGC CAAGAAGGGC CGTCCCATGG TGGTGTCCAG CGGGATGCAG 
ACTATGGAGA CGATGCGTCA GGTCTACAAG ACTGTGAAAC AGCACAATAA AAACTTTGCC 
ATCCTTCAGT GCACCAGTGC GTATCCTCTG GAGGCTGAAG ATGTCAACCT CAAAGTGATC 
GCGGAATACC AGAAAGAGTT TCCTGATATT CCAATCGGGT ATTCAGGTCA CGAGTCTGGA 
ATCACTATTT CTGTGGGAGC CGTAGCTCTG GGAGCGAAGG TCCTGGAGCG TCACATAACC 
TTGGACAAGT TTTGGAAAGG AAGCGACCAT GAGGCCTCGT TGATACCGTC TGAACTGGCT 
GAGCTGGTCC GCTCTGTTCG TCTGATGGAG AGGGCACTGG GAAATGGCAT CAAGCAGATG 
TTGCCCTGTG AGAAGGCCTG CCATGACAAA CTTGGCAAAT CTGTGGTGGC GAAGGTGAAG 
ATCCCCAAAG GAACAGTCCT GACTCTGGAC ATGTTGGGGG TGAAGGTGGC TGAGCCGATG 
GGTGTCCCGG CAGAGGACAT CTTCCAGCTG GTTGGGAAGA CTGTGAAGGA GGAGGTGGAG 
GAGGATGAGA GTGTTCAGCC TGAAGTGGTG GACCGCTATG GCAAGAAGGC CAAGTGTTAA