znf574 cDNA ORF clone, Cynoglossus semilaevis(tongue sole)

Gene
Clones
gRNAs

Summary

Gene Symbol	znf574
Entrez Gene ID	103387998
Full Name	zinc finger protein 574
Gene Type	protein-coding
Organism	Cynoglossus semilaevis(tongue sole)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008322835.2	XP_008321057.1	zinc finger protein 574
XM_025061262.1	XP_024917030.1	zinc finger protein 574

Pathways from BioSystems
Gene Ontology

The following znf574 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the znf574 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCy196003	XM_025061262.1 Latest version!	Cynoglossus semilaevis zinc finger protein 574 (znf574), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OCy40439	XM_008322835.2 Latest version!	Cynoglossus semilaevis zinc finger protein 574 (znf574), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

znf574 ( XM_025061262.1 ) cDNA ORF clone, Cynoglossus semilaevis(tongue sole) -> XP_024917030.1 Cynoglossus semilaevis zinc finger protein 574 (znf574), mRNA.

Price & Availability↑

CloneID	OCy196003
Clone ID Related Accession (Same CDS sequence)	XM_025061262.1
Accession Version	XM_025061262.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-15
Organism	Cynoglossus semilaevis(tongue sole)
Product	zinc finger protein 574
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024319.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cynoglossus semilaevis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC AACAGGAGGA GGCAGAGAGT GCATGTGTGG AGCACCAGTA CATGTGCAGT 
GAGTGCCAGC AGCTCTTCAA CACCCTGGAG GATGTGTTGG TCCACCAACG CATTCACACC 
GGACAAGACG CAGACGGAGA AGTGGAGATT GTCGAGGGTG TGACCGTGCA GGGTGTTTTA 
GATGAGGGTC AGACCCAGAC CTACCAGTGT CTGGAGTGTG GAGCCCTCAT CATCAACCCC 
GAGGAGCTGC TGCAGCACCA GGAGATGCAC ATGAGGGAGG CTGAGATGGA GGTCGAGCAA 
CAAGGTCTAA CTGAGGTCCT GGAAACACAG GCAGGTCCAG CAGAGACTCA GGGGTCTGCG 
GGGGTCCAGT ACCAATGTCT GGACTGTCTG GCTCTGTTCG ACTCACCCGA CACCTGGTTG 
GAGCATCGTC GAATGCACAA CAGGAGCAGC ACGCACAGTA ACACAGAGTC CTTGGAGTAC 
GTGATTCAGC CCAATGGAAC TCTCACCCCA CTGAACAGTA TGCAAAACTA CCTGTTGAGT 
GAGCAGCAGG CTGGAGAGAT ACTGGCACAG GTTCTTGCTC AGCAACAGCA ACAACGACAA 
CAACAAAGGA AACATCGACC CGTCTCTAAA GCTGCGGCCT CTCGCTCCAC CCTGCTGCCT 
CCCGTCACGC CCACCCCCGG CTCTGCTACC ATGCACCTGC AGATTCTCAC TGCACAGGTT 
CTGGCCGACA ACTCTGCTCC CAGTCAGCAG CATTCCAAGA TGAGCCAAGG CGCCGCGGCC 
AAGCTCGGAG TCCTGGAGAA CGGTGTCCAG AGACTGGAGC TGAGGTTGGC CCCCGGGGTC 
AAGGAGCTGC AGCAGCCAAC TGAGGTGGTG GTCATCCACC CCTACGAATG CTCCGAGTGC 
TCCCTCCTAT TCCAGACGCC GGAGGATTTC CTCCAGCACC AGGGGGAGCA CTTTCTATGT 
CAGGACAAAG AAAGCGGGGA GTCGGGGCTC ATGAGTGGAT TTGACGAGAG GGGGAGGGAG 
GAGGCACTGG GGAAAGGAGA AGATATTCGA GGGGCAGATA ACAGTGGGGC GGTGTGGGCA 
AAGTCTCAGA AGTGTGACCT GTGCAGTCGC ACCTTCAACT CGGTCAACCG GCTGGCGGCT 
CACAAGCGCG TCCACGAGCA GGGCACTCAC GAATGCCCCG AGTGTGGAAA AGTGTTCAAA 
AAGGTCACGT CGCTGCAGAC GCACCAGCGC ACACACTCTG GAGTGGCCAG GTATCTGTGT 
GTGGACTGTG GCAACGGCTA CACCACAGAG ATGACGCTCA TCATGCACAG GAAGTCCCAC 
ACTTCTGACC CTCTTCACAA GTGTCAGTTC TGTAACAAGA CCTTCACCAA CATGACCAAG 
TACCTGTACC ACCGTCGCAC CCACCTGAAC CGGGACGCCA CTGGAACACC GGCCGTTTTC 
ACGGACTCTG CTCCAACACG AGCGTCTCTG TCGGCCCTGT CCATCCTCCA GAGAGCCAGA 
GGAAAGAAGA ACCACCAACG GGTGGAAGAC GTCCACGCAC TGGAGCCGTT CTTTCAGGAC 
CAGACGGAAA AAGTGGAACC AGAACGGTGT GTTCAGGTCC AGGAAGAAGG TGAAGTTGAG 
AAGCAGAGTG AGGAGGAAAA CATGGAGGTG TCCTCTCCAC CTGAAGAGAA GACTCAAGCC 
CCGCCCCCAG GCCAGGCTTC AGCTGGAAAC CACACCTCAG CGCCGACCAC AGACTGTACA 
GCAGGAGCTC CAGCTCCACC CTCTGAGGGG GGGGCCATTT CCTGTCAAAA GTGCAACAAA 
AGCTTTTCCT CCCAGCTGCA GTTGGTTCAA CACGGACTCA CGGCCCACGT CGCTGAGCGC 
AGCTTTGTCT GTGGAATCTG CGGCAAGTCT TTCAAGAAGC AGCTCCACGT CCGTAACCAC 
ATACGGACGC ACACGGGGGA ACGGCCGTTC CAGTGTTCAG ACTGCGGCAA GACCTTCTCC 
TTCCTGGCCA ATCTGATGAG ACACAACCTG ATCCACTCCG GCGTCCGTCC GTATCGCTGT 
GACGTCTGCC ACCGCACCTT CTCCCAGTCC TCCAACCTCC GTCAGCACAG CCTGCTGCAC 
TCCAAAGCTG CCACCCTGAG GTGCCCCGAC TGCCCCAGCA CCTTCCGCTG GCCCACCAAG 
TTAGCAGCAC ATCGCTACAC CCAGCACCCG GGGTCTCCAG CGCCCTTCAC CTGCCCTCAC 
TGTGCAGCCG GCTTCTTGAG TCGACGACAG CGAGACGCCC ACTGCCTGGA GCAGCACCTC 
AGCCTGGTGC AGGCTGCTGC AGGGAAAGAA GTCAACACCA GGGGCGCGGA CCAATCGCAG 
CCGGACAAGG AGCTCACCGC CGATCCCTCC GCCTCAACTG CAGCCCAGGG TGACGCGACA 
GCTTCTGTGT GGGGGGGGTT AGGTTGTAAC ATCTGTGGGA AGAAGCTCAA CTCTGCAGCC 
AATCTGCGTC TTCACAAACT GAGCCACTTC GCCGCAGGCC CCGGGCGACC GCGGCCCACC 
ATGGTGAAGC GGGCCCAGAA GTGCCCCGTC TGTGGGAAAC AGTTTGTTTC TTCCTCTGGA 
GTCGCGCTTC ACCAGCGCGT TCACACCGGC GAACGGCCGT TTCCCTGTCA GGTGTGCGGC 
AAACGTTTTC GTCAGAACAC GCATCTGCGC GAGCACCTCC GCACGCACTC CGGTGAGCGG 
CCGTTCCGCT GCGAGGTTTG TGGGAAAGGG TTCATCCAGA GCATGCACCT GGCTGAACAC 
CGGCGTACGC ACACGGGCGA ACGACCTCAC GTCTGCCCCC TGTGTGGCAA AGCATTTAAG 
ACCTTCTCCA ACCTGAGGAA CCACAAAAAG ACCCACGTCA GGCAGCAGAA GCTGGACCAG 
GAAGCTGCTG CTGCTGATGC TGATGCTGAT GCCCAGGCTG CTGCTGATGC TCAGGCCGCT 
GCTGATGCTC AGGCCGCTGC TGATGCTCGG GCTGCTGATG CCCAGGCCAC CATGCAGACT 
TCTCCTGTGA CAACGTTGGA TGCCTCAACT GTAGAAGTGG CCGACAGTCA ACCTCAGATC 
ATCCAAATTC ACGCCTCTGA TATCCCTCAG GGCACTCCTA CCATCATGTG CAATGAGTTT 
GGAGAGACCA TCGCCATCAT CGAGACCAGC GAGGGGGGGG AGCTGCCCCT GGAACAGGCC 
CTGCAGATCT ACCACACGGC CCTGGAGAAC GGCCTGGCCG TCGACACGGT GGCTGTGGAT 
GGACTGCAGC TCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024917030.1
CDS	441..3698
Translation	MEEQQEEAESACVEHQYMCSECQQLFNTLEDVLVHQRIHTGQDADGEVEIVEGVTVQGVLDEGQTQTYQCLECGALIINPEELLQHQEMHMREAEMEVEQQGLTEVLETQAGPAETQGSAGVQYQCLDCLALFDSPDTWLEHRRMHNRSSTHSNTESLEYVIQPNGTLTPLNSMQNYLLSEQQAGEILAQVLAQQQQQRQQQRKHRPVSKAAASRSTLLPPVTPTPGSATMHLQILTAQVLADNSAPSQQHSKMSQGAAAKLGVLENGVQRLELRLAPGVKELQQPTEVVVIHPYECSECSLLFQTPEDFLQHQGEHFLCQDKESGESGLMSGFDERGREEALGKGEDIRGADNSGAVWAKSQKCDLCSRTFNSVNRLAAHKRVHEQGTHECPECGKVFKKVTSLQTHQRTHSGVARYLCVDCGNGYTTEMTLIMHRKSHTSDPLHKCQFCNKTFTNMTKYLYHRRTHLNRDATGTPAVFTDSAPTRASLSALSILQRARGKKNHQRVEDVHALEPFFQDQTEKVEPERCVQVQEEGEVEKQSEEENMEVSSPPEEKTQAPPPGQASAGNHTSAPTTDCTAGAPAPPSEGGAISCQKCNKSFSSQLQLVQHGLTAHVAERSFVCGICGKSFKKQLHVRNHIRTHTGERPFQCSDCGKTFSFLANLMRHNLIHSGVRPYRCDVCHRTFSQSSNLRQHSLLHSKAATLRCPDCPSTFRWPTKLAAHRYTQHPGSPAPFTCPHCAAGFLSRRQRDAHCLEQHLSLVQAAAGKEVNTRGADQSQPDKELTADPSASTAAQGDATASVWGGLGCNICGKKLNSAANLRLHKLSHFAAGPGRPRPTMVKRAQKCPVCGKQFVSSSGVALHQRVHTGERPFPCQVCGKRFRQNTHLREHLRTHSGERPFRCEVCGKGFIQSMHLAEHRRTHTGERPHVCPLCGKAFKTFSNLRNHKKTHVRQQKLDQEAAAADADADAQAAADAQAAADAQAAADARAADAQATMQTSPVTTLDASTVEVADSQPQIIQIHASDIPQGTPTIMCNEFGETIAIIETSEGGELPLEQALQIYHTALENGLAVDTVAVDGLQLL

Target ORF information:

RefSeq Version	XM_025061262.1
Organism	Cynoglossus semilaevis(tongue sole)
Definition	Cynoglossus semilaevis zinc finger protein 574 (znf574), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025061262.1

ORF Insert Sequence:

ATGGAGGAGC AACAGGAGGA GGCAGAGAGT GCATGTGTGG AGCACCAGTA CATGTGCAGT 
GAGTGCCAGC AGCTCTTCAA CACCCTGGAG GATGTGTTGG TCCACCAACG CATTCACACC 
GGACAAGACG CAGACGGAGA AGTGGAGATT GTCGAGGGTG TGACCGTGCA GGGTGTTTTA 
GATGAGGGTC AGACCCAGAC CTACCAGTGT CTGGAGTGTG GAGCCCTCAT CATCAACCCC 
GAGGAGCTGC TGCAGCACCA GGAGATGCAC ATGAGGGAGG CTGAGATGGA GGTCGAGCAA 
CAAGGTCTAA CTGAGGTCCT GGAAACACAG GCAGGTCCAG CAGAGACTCA GGGGTCTGCG 
GGGGTCCAGT ACCAATGTCT GGACTGTCTG GCTCTGTTCG ACTCACCCGA CACCTGGTTG 
GAGCATCGTC GAATGCACAA CAGGAGCAGC ACGCACAGTA ACACAGAGTC CTTGGAGTAC 
GTGATTCAGC CCAATGGAAC TCTCACCCCA CTGAACAGTA TGCAAAACTA CCTGTTGAGT 
GAGCAGCAGG CTGGAGAGAT ACTGGCACAG GTTCTTGCTC AGCAACAGCA ACAACGACAA 
CAACAAAGGA AACATCGACC CGTCTCTAAA GCTGCGGCCT CTCGCTCCAC CCTGCTGCCT 
CCCGTCACGC CCACCCCCGG CTCTGCTACC ATGCACCTGC AGATTCTCAC TGCACAGGTT 
CTGGCCGACA ACTCTGCTCC CAGTCAGCAG CATTCCAAGA TGAGCCAAGG CGCCGCGGCC 
AAGCTCGGAG TCCTGGAGAA CGGTGTCCAG AGACTGGAGC TGAGGTTGGC CCCCGGGGTC 
AAGGAGCTGC AGCAGCCAAC TGAGGTGGTG GTCATCCACC CCTACGAATG CTCCGAGTGC 
TCCCTCCTAT TCCAGACGCC GGAGGATTTC CTCCAGCACC AGGGGGAGCA CTTTCTATGT 
CAGGACAAAG AAAGCGGGGA GTCGGGGCTC ATGAGTGGAT TTGACGAGAG GGGGAGGGAG 
GAGGCACTGG GGAAAGGAGA AGATATTCGA GGGGCAGATA ACAGTGGGGC GGTGTGGGCA 
AAGTCTCAGA AGTGTGACCT GTGCAGTCGC ACCTTCAACT CGGTCAACCG GCTGGCGGCT 
CACAAGCGCG TCCACGAGCA GGGCACTCAC GAATGCCCCG AGTGTGGAAA AGTGTTCAAA 
AAGGTCACGT CGCTGCAGAC GCACCAGCGC ACACACTCTG GAGTGGCCAG GTATCTGTGT 
GTGGACTGTG GCAACGGCTA CACCACAGAG ATGACGCTCA TCATGCACAG GAAGTCCCAC 
ACTTCTGACC CTCTTCACAA GTGTCAGTTC TGTAACAAGA CCTTCACCAA CATGACCAAG 
TACCTGTACC ACCGTCGCAC CCACCTGAAC CGGGACGCCA CTGGAACACC GGCCGTTTTC 
ACGGACTCTG CTCCAACACG AGCGTCTCTG TCGGCCCTGT CCATCCTCCA GAGAGCCAGA 
GGAAAGAAGA ACCACCAACG GGTGGAAGAC GTCCACGCAC TGGAGCCGTT CTTTCAGGAC 
CAGACGGAAA AAGTGGAACC AGAACGGTGT GTTCAGGTCC AGGAAGAAGG TGAAGTTGAG 
AAGCAGAGTG AGGAGGAAAA CATGGAGGTG TCCTCTCCAC CTGAAGAGAA GACTCAAGCC 
CCGCCCCCAG GCCAGGCTTC AGCTGGAAAC CACACCTCAG CGCCGACCAC AGACTGTACA 
GCAGGAGCTC CAGCTCCACC CTCTGAGGGG GGGGCCATTT CCTGTCAAAA GTGCAACAAA 
AGCTTTTCCT CCCAGCTGCA GTTGGTTCAA CACGGACTCA CGGCCCACGT CGCTGAGCGC 
AGCTTTGTCT GTGGAATCTG CGGCAAGTCT TTCAAGAAGC AGCTCCACGT CCGTAACCAC 
ATACGGACGC ACACGGGGGA ACGGCCGTTC CAGTGTTCAG ACTGCGGCAA GACCTTCTCC 
TTCCTGGCCA ATCTGATGAG ACACAACCTG ATCCACTCCG GCGTCCGTCC GTATCGCTGT 
GACGTCTGCC ACCGCACCTT CTCCCAGTCC TCCAACCTCC GTCAGCACAG CCTGCTGCAC 
TCCAAAGCTG CCACCCTGAG GTGCCCCGAC TGCCCCAGCA CCTTCCGCTG GCCCACCAAG 
TTAGCAGCAC ATCGCTACAC CCAGCACCCG GGGTCTCCAG CGCCCTTCAC CTGCCCTCAC 
TGTGCAGCCG GCTTCTTGAG TCGACGACAG CGAGACGCCC ACTGCCTGGA GCAGCACCTC 
AGCCTGGTGC AGGCTGCTGC AGGGAAAGAA GTCAACACCA GGGGCGCGGA CCAATCGCAG 
CCGGACAAGG AGCTCACCGC CGATCCCTCC GCCTCAACTG CAGCCCAGGG TGACGCGACA 
GCTTCTGTGT GGGGGGGGTT AGGTTGTAAC ATCTGTGGGA AGAAGCTCAA CTCTGCAGCC 
AATCTGCGTC TTCACAAACT GAGCCACTTC GCCGCAGGCC CCGGGCGACC GCGGCCCACC 
ATGGTGAAGC GGGCCCAGAA GTGCCCCGTC TGTGGGAAAC AGTTTGTTTC TTCCTCTGGA 
GTCGCGCTTC ACCAGCGCGT TCACACCGGC GAACGGCCGT TTCCCTGTCA GGTGTGCGGC 
AAACGTTTTC GTCAGAACAC GCATCTGCGC GAGCACCTCC GCACGCACTC CGGTGAGCGG 
CCGTTCCGCT GCGAGGTTTG TGGGAAAGGG TTCATCCAGA GCATGCACCT GGCTGAACAC 
CGGCGTACGC ACACGGGCGA ACGACCTCAC GTCTGCCCCC TGTGTGGCAA AGCATTTAAG 
ACCTTCTCCA ACCTGAGGAA CCACAAAAAG ACCCACGTCA GGCAGCAGAA GCTGGACCAG 
GAAGCTGCTG CTGCTGATGC TGATGCTGAT GCCCAGGCTG CTGCTGATGC TCAGGCCGCT 
GCTGATGCTC AGGCCGCTGC TGATGCTCGG GCTGCTGATG CCCAGGCCAC CATGCAGACT 
TCTCCTGTGA CAACGTTGGA TGCCTCAACT GTAGAAGTGG CCGACAGTCA ACCTCAGATC 
ATCCAAATTC ACGCCTCTGA TATCCCTCAG GGCACTCCTA CCATCATGTG CAATGAGTTT 
GGAGAGACCA TCGCCATCAT CGAGACCAGC GAGGGGGGGG AGCTGCCCCT GGAACAGGCC 
CTGCAGATCT ACCACACGGC CCTGGAGAAC GGCCTGGCCG TCGACACGGT GGCTGTGGAT 
GGACTGCAGC TCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

znf574 ( XM_008322835.2 ) cDNA ORF clone, Cynoglossus semilaevis(tongue sole) -> XP_008321057.1 Cynoglossus semilaevis zinc finger protein 574 (znf574), mRNA.

Price & Availability↑

CloneID	OCy40439
Clone ID Related Accession (Same CDS sequence)	XM_008322835.2
Accession Version	XM_008322835.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-06
Organism	Cynoglossus semilaevis(tongue sole)
Product	zinc finger protein 574
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024319.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 7, 2016 this sequence version replaced XM_008322835.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cynoglossus semilaevis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC AACAGGAGGA GGCAGAGAGT GCATGTGTGG AGCACCAGTA CATGTGCAGT 
GAGTGCCAGC AGCTCTTCAA CACCCTGGAG GATGTGTTGG TCCACCAACG CATTCACACC 
GGACAAGACG CAGACGGAGA AGTGGAGATT GTCGAGGGTG TGACCGTGCA GGGTGTTTTA 
GATGAGGGTC AGACCCAGAC CTACCAGTGT CTGGAGTGTG GAGCCCTCAT CATCAACCCC 
GAGGAGCTGC TGCAGCACCA GGAGATGCAC ATGAGGGAGG CTGAGATGGA GGTCGAGCAA 
CAAGGTCTAA CTGAGGTCCT GGAAACACAG GCAGGTCCAG CAGAGACTCA GGGGTCTGCG 
GGGGTCCAGT ACCAATGTCT GGACTGTCTG GCTCTGTTCG ACTCACCCGA CACCTGGTTG 
GAGCATCGTC GAATGCACAA CAGGAGCAGC ACGCACAGTA ACACAGAGTC CTTGGAGTAC 
GTGATTCAGC CCAATGGAAC TCTCACCCCA CTGAACAGTA TGCAAAACTA CCTGTTGAGT 
GAGCAGCAGG CTGGAGAGAT ACTGGCACAG GTTCTTGCTC AGCAACAGCA ACAACGACAA 
CAACAAAGGA AACATCGACC CGTCTCTAAA GCTGCGGCCT CTCGCTCCAC CCTGCTGCCT 
CCCGTCACGC CCACCCCCGG CTCTGCTACC ATGCACCTGC AGATTCTCAC TGCACAGGTT 
CTGGCCGACA ACTCTGCTCC CAGTCAGCAG CATTCCAAGA TGAGCCAAGG CGCCGCGGCC 
AAGCTCGGAG TCCTGGAGAA CGGTGTCCAG AGACTGGAGC TGAGGTTGGC CCCCGGGGTC 
AAGGAGCTGC AGCAGCCAAC TGAGGTGGTG GTCATCCACC CCTACGAATG CTCCGAGTGC 
TCCCTCCTAT TCCAGACGCC GGAGGATTTC CTCCAGCACC AGGGGGAGCA CTTTCTATGT 
CAGGACAAAG AAAGCGGGGA GTCGGGGCTC ATGAGTGGAT TTGACGAGAG GGGGAGGGAG 
GAGGCACTGG GGAAAGGAGA AGATATTCGA GGGGCAGATA ACAGTGGGGC GGTGTGGGCA 
AAGTCTCAGA AGTGTGACCT GTGCAGTCGC ACCTTCAACT CGGTCAACCG GCTGGCGGCT 
CACAAGCGCG TCCACGAGCA GGGCACTCAC GAATGCCCCG AGTGTGGAAA AGTGTTCAAA 
AAGGTCACGT CGCTGCAGAC GCACCAGCGC ACACACTCTG GAGTGGCCAG GTATCTGTGT 
GTGGACTGTG GCAACGGCTA CACCACAGAG ATGACGCTCA TCATGCACAG GAAGTCCCAC 
ACTTCTGACC CTCTTCACAA GTGTCAGTTC TGTAACAAGA CCTTCACCAA CATGACCAAG 
TACCTGTACC ACCGTCGCAC CCACCTGAAC CGGGACGCCA CTGGAACACC GGCCGTTTTC 
ACGGACTCTG CTCCAACACG AGCGTCTCTG TCGGCCCTGT CCATCCTCCA GAGAGCCAGA 
GGAAAGAAGA ACCACCAACG GGTGGAAGAC GTCCACGCAC TGGAGCCGTT CTTTCAGGAC 
CAGACGGAAA AAGTGGAACC AGAACGGTGT GTTCAGGTCC AGGAAGAAGG TGAAGTTGAG 
AAGCAGAGTG AGGAGGAAAA CATGGAGGTG TCCTCTCCAC CTGAAGAGAA GACTCAAGCC 
CCGCCCCCAG GCCAGGCTTC AGCTGGAAAC CACACCTCAG CGCCGACCAC AGACTGTACA 
GCAGGAGCTC CAGCTCCACC CTCTGAGGGG GGGGCCATTT CCTGTCAAAA GTGCAACAAA 
AGCTTTTCCT CCCAGCTGCA GTTGGTTCAA CACGGACTCA CGGCCCACGT CGCTGAGCGC 
AGCTTTGTCT GTGGAATCTG CGGCAAGTCT TTCAAGAAGC AGCTCCACGT CCGTAACCAC 
ATACGGACGC ACACGGGGGA ACGGCCGTTC CAGTGTTCAG ACTGCGGCAA GACCTTCTCC 
TTCCTGGCCA ATCTGATGAG ACACAACCTG ATCCACTCCG GCGTCCGTCC GTATCGCTGT 
GACGTCTGCC ACCGCACCTT CTCCCAGTCC TCCAACCTCC GTCAGCACAG CCTGCTGCAC 
TCCAAAGCTG CCACCCTGAG GTGCCCCGAC TGCCCCAGCA CCTTCCGCTG GCCCACCAAG 
TTAGCAGCAC ATCGCTACAC CCAGCACCCG GGGTCTCCAG CGCCCTTCAC CTGCCCTCAC 
TGTGCAGCCG GCTTCTTGAG TCGACGACAG CGAGACGCCC ACTGCCTGGA GCAGCACCTC 
AGCCTGGTGC AGGCTGCTGC AGGGAAAGAA GTCAACACCA GGGGCGCGGA CCAATCGCAG 
CCGGACAAGG AGCTCACCGC CGATCCCTCC GCCTCAACTG CAGCCCAGGG TGACGCGACA 
GCTTCTGTGT GGGGGGGGTT AGGTTGTAAC ATCTGTGGGA AGAAGCTCAA CTCTGCAGCC 
AATCTGCGTC TTCACAAACT GAGCCACTTC GCCGCAGGCC CCGGGCGACC GCGGCCCACC 
ATGGTGAAGC GGGCCCAGAA GTGCCCCGTC TGTGGGAAAC AGTTTGTTTC TTCCTCTGGA 
GTCGCGCTTC ACCAGCGCGT TCACACCGGC GAACGGCCGT TTCCCTGTCA GGTGTGCGGC 
AAACGTTTTC GTCAGAACAC GCATCTGCGC GAGCACCTCC GCACGCACTC CGGTGAGCGG 
CCGTTCCGCT GCGAGGTTTG TGGGAAAGGG TTCATCCAGA GCATGCACCT GGCTGAACAC 
CGGCGTACGC ACACGGGCGA ACGACCTCAC GTCTGCCCCC TGTGTGGCAA AGCATTTAAG 
ACCTTCTCCA ACCTGAGGAA CCACAAAAAG ACCCACGTCA GGCAGCAGAA GCTGGACCAG 
GAAGCTGCTG CTGCTGATGC TGATGCTGAT GCCCAGGCTG CTGCTGATGC TCAGGCCGCT 
GCTGATGCTC AGGCCGCTGC TGATGCTCGG GCTGCTGATG CCCAGGCCAC CATGCAGACT 
TCTCCTGTGA CAACGTTGGA TGCCTCAACT GTAGAAGTGG CCGACAGTCA ACCTCAGATC 
ATCCAAATTC ACGCCTCTGA TATCCTCGGG GGCACTCCTA CCATCATGTG CAATGAGTTT 
GGAGAGACCA TCGCCATCAT CGAGACCAGC GAGGGGGGGG AGCTGCCCCT GGAACAGGCC 
CTGCAGATCT ACCACACGGC CCTGGAGAAC GGCCTGGCCG TCGACACGGT GGCTGTGGAT 
GGACTGCAGC TCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008321057.1
CDS	332..3589
Translation	MEEQQEEAESACVEHQYMCSECQQLFNTLEDVLVHQRIHTGQDADGEVEIVEGVTVQGVLDEGQTQTYQCLECGALIINPEELLQHQEMHMREAEMEVEQQGLTEVLETQAGPAETQGSAGVQYQCLDCLALFDSPDTWLEHRRMHNRSSTHSNTESLEYVIQPNGTLTPLNSMQNYLLSEQQAGEILAQVLAQQQQQRQQQRKHRPVSKAAASRSTLLPPVTPTPGSATMHLQILTAQVLADNSAPSQQHSKMSQGAAAKLGVLENGVQRLELRLAPGVKELQQPTEVVVIHPYECSECSLLFQTPEDFLQHQGEHFLCQDKESGESGLMSGFDERGREEALGKGEDIRGADNSGAVWAKSQKCDLCSRTFNSVNRLAAHKRVHEQGTHECPECGKVFKKVTSLQTHQRTHSGVARYLCVDCGNGYTTEMTLIMHRKSHTSDPLHKCQFCNKTFTNMTKYLYHRRTHLNRDATGTPAVFTDSAPTRASLSALSILQRARGKKNHQRVEDVHALEPFFQDQTEKVEPERCVQVQEEGEVEKQSEEENMEVSSPPEEKTQAPPPGQASAGNHTSAPTTDCTAGAPAPPSEGGAISCQKCNKSFSSQLQLVQHGLTAHVAERSFVCGICGKSFKKQLHVRNHIRTHTGERPFQCSDCGKTFSFLANLMRHNLIHSGVRPYRCDVCHRTFSQSSNLRQHSLLHSKAATLRCPDCPSTFRWPTKLAAHRYTQHPGSPAPFTCPHCAAGFLSRRQRDAHCLEQHLSLVQAAAGKEVNTRGADQSQPDKELTADPSASTAAQGDATASVWGGLGCNICGKKLNSAANLRLHKLSHFAAGPGRPRPTMVKRAQKCPVCGKQFVSSSGVALHQRVHTGERPFPCQVCGKRFRQNTHLREHLRTHSGERPFRCEVCGKGFIQSMHLAEHRRTHTGERPHVCPLCGKAFKTFSNLRNHKKTHVRQQKLDQEAAAADADADAQAAADAQAAADAQAAADARAADAQATMQTSPVTTLDASTVEVADSQPQIIQIHASDILGGTPTIMCNEFGETIAIIETSEGGELPLEQALQIYHTALENGLAVDTVAVDGLQLL

Target ORF information:

RefSeq Version	XM_008322835.2
Organism	Cynoglossus semilaevis(tongue sole)
Definition	Cynoglossus semilaevis zinc finger protein 574 (znf574), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008322835.2

ORF Insert Sequence:

ATGGAGGAGC AACAGGAGGA GGCAGAGAGT GCATGTGTGG AGCACCAGTA CATGTGCAGT 
GAGTGCCAGC AGCTCTTCAA CACCCTGGAG GATGTGTTGG TCCACCAACG CATTCACACC 
GGACAAGACG CAGACGGAGA AGTGGAGATT GTCGAGGGTG TGACCGTGCA GGGTGTTTTA 
GATGAGGGTC AGACCCAGAC CTACCAGTGT CTGGAGTGTG GAGCCCTCAT CATCAACCCC 
GAGGAGCTGC TGCAGCACCA GGAGATGCAC ATGAGGGAGG CTGAGATGGA GGTCGAGCAA 
CAAGGTCTAA CTGAGGTCCT GGAAACACAG GCAGGTCCAG CAGAGACTCA GGGGTCTGCG 
GGGGTCCAGT ACCAATGTCT GGACTGTCTG GCTCTGTTCG ACTCACCCGA CACCTGGTTG 
GAGCATCGTC GAATGCACAA CAGGAGCAGC ACGCACAGTA ACACAGAGTC CTTGGAGTAC 
GTGATTCAGC CCAATGGAAC TCTCACCCCA CTGAACAGTA TGCAAAACTA CCTGTTGAGT 
GAGCAGCAGG CTGGAGAGAT ACTGGCACAG GTTCTTGCTC AGCAACAGCA ACAACGACAA 
CAACAAAGGA AACATCGACC CGTCTCTAAA GCTGCGGCCT CTCGCTCCAC CCTGCTGCCT 
CCCGTCACGC CCACCCCCGG CTCTGCTACC ATGCACCTGC AGATTCTCAC TGCACAGGTT 
CTGGCCGACA ACTCTGCTCC CAGTCAGCAG CATTCCAAGA TGAGCCAAGG CGCCGCGGCC 
AAGCTCGGAG TCCTGGAGAA CGGTGTCCAG AGACTGGAGC TGAGGTTGGC CCCCGGGGTC 
AAGGAGCTGC AGCAGCCAAC TGAGGTGGTG GTCATCCACC CCTACGAATG CTCCGAGTGC 
TCCCTCCTAT TCCAGACGCC GGAGGATTTC CTCCAGCACC AGGGGGAGCA CTTTCTATGT 
CAGGACAAAG AAAGCGGGGA GTCGGGGCTC ATGAGTGGAT TTGACGAGAG GGGGAGGGAG 
GAGGCACTGG GGAAAGGAGA AGATATTCGA GGGGCAGATA ACAGTGGGGC GGTGTGGGCA 
AAGTCTCAGA AGTGTGACCT GTGCAGTCGC ACCTTCAACT CGGTCAACCG GCTGGCGGCT 
CACAAGCGCG TCCACGAGCA GGGCACTCAC GAATGCCCCG AGTGTGGAAA AGTGTTCAAA 
AAGGTCACGT CGCTGCAGAC GCACCAGCGC ACACACTCTG GAGTGGCCAG GTATCTGTGT 
GTGGACTGTG GCAACGGCTA CACCACAGAG ATGACGCTCA TCATGCACAG GAAGTCCCAC 
ACTTCTGACC CTCTTCACAA GTGTCAGTTC TGTAACAAGA CCTTCACCAA CATGACCAAG 
TACCTGTACC ACCGTCGCAC CCACCTGAAC CGGGACGCCA CTGGAACACC GGCCGTTTTC 
ACGGACTCTG CTCCAACACG AGCGTCTCTG TCGGCCCTGT CCATCCTCCA GAGAGCCAGA 
GGAAAGAAGA ACCACCAACG GGTGGAAGAC GTCCACGCAC TGGAGCCGTT CTTTCAGGAC 
CAGACGGAAA AAGTGGAACC AGAACGGTGT GTTCAGGTCC AGGAAGAAGG TGAAGTTGAG 
AAGCAGAGTG AGGAGGAAAA CATGGAGGTG TCCTCTCCAC CTGAAGAGAA GACTCAAGCC 
CCGCCCCCAG GCCAGGCTTC AGCTGGAAAC CACACCTCAG CGCCGACCAC AGACTGTACA 
GCAGGAGCTC CAGCTCCACC CTCTGAGGGG GGGGCCATTT CCTGTCAAAA GTGCAACAAA 
AGCTTTTCCT CCCAGCTGCA GTTGGTTCAA CACGGACTCA CGGCCCACGT CGCTGAGCGC 
AGCTTTGTCT GTGGAATCTG CGGCAAGTCT TTCAAGAAGC AGCTCCACGT CCGTAACCAC 
ATACGGACGC ACACGGGGGA ACGGCCGTTC CAGTGTTCAG ACTGCGGCAA GACCTTCTCC 
TTCCTGGCCA ATCTGATGAG ACACAACCTG ATCCACTCCG GCGTCCGTCC GTATCGCTGT 
GACGTCTGCC ACCGCACCTT CTCCCAGTCC TCCAACCTCC GTCAGCACAG CCTGCTGCAC 
TCCAAAGCTG CCACCCTGAG GTGCCCCGAC TGCCCCAGCA CCTTCCGCTG GCCCACCAAG 
TTAGCAGCAC ATCGCTACAC CCAGCACCCG GGGTCTCCAG CGCCCTTCAC CTGCCCTCAC 
TGTGCAGCCG GCTTCTTGAG TCGACGACAG CGAGACGCCC ACTGCCTGGA GCAGCACCTC 
AGCCTGGTGC AGGCTGCTGC AGGGAAAGAA GTCAACACCA GGGGCGCGGA CCAATCGCAG 
CCGGACAAGG AGCTCACCGC CGATCCCTCC GCCTCAACTG CAGCCCAGGG TGACGCGACA 
GCTTCTGTGT GGGGGGGGTT AGGTTGTAAC ATCTGTGGGA AGAAGCTCAA CTCTGCAGCC 
AATCTGCGTC TTCACAAACT GAGCCACTTC GCCGCAGGCC CCGGGCGACC GCGGCCCACC 
ATGGTGAAGC GGGCCCAGAA GTGCCCCGTC TGTGGGAAAC AGTTTGTTTC TTCCTCTGGA 
GTCGCGCTTC ACCAGCGCGT TCACACCGGC GAACGGCCGT TTCCCTGTCA GGTGTGCGGC 
AAACGTTTTC GTCAGAACAC GCATCTGCGC GAGCACCTCC GCACGCACTC CGGTGAGCGG 
CCGTTCCGCT GCGAGGTTTG TGGGAAAGGG TTCATCCAGA GCATGCACCT GGCTGAACAC 
CGGCGTACGC ACACGGGCGA ACGACCTCAC GTCTGCCCCC TGTGTGGCAA AGCATTTAAG 
ACCTTCTCCA ACCTGAGGAA CCACAAAAAG ACCCACGTCA GGCAGCAGAA GCTGGACCAG 
GAAGCTGCTG CTGCTGATGC TGATGCTGAT GCCCAGGCTG CTGCTGATGC TCAGGCCGCT 
GCTGATGCTC AGGCCGCTGC TGATGCTCGG GCTGCTGATG CCCAGGCCAC CATGCAGACT 
TCTCCTGTGA CAACGTTGGA TGCCTCAACT GTAGAAGTGG CCGACAGTCA ACCTCAGATC 
ATCCAAATTC ACGCCTCTGA TATCCTCGGG GGCACTCCTA CCATCATGTG CAATGAGTTT 
GGAGAGACCA TCGCCATCAT CGAGACCAGC GAGGGGGGGG AGCTGCCCCT GGAACAGGCC 
CTGCAGATCT ACCACACGGC CCTGGAGAAC GGCCTGGCCG TCGACACGGT GGCTGTGGAT 
GGACTGCAGC TCCTCTGA