LOC103387313 cDNA ORF clone, Cynoglossus semilaevis(tongue sole)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC103387313
Entrez Gene ID	103387313
Full Name	WASH complex subunit FAM21-like
Gene Type	protein-coding
Organism	Cynoglossus semilaevis(tongue sole)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017037518.1	XP_016893007.1	WASH complex subunit FAM21-like isoform X3
XM_008321893.3	XP_008320115.1	WASH complex subunit 2-like isoform X2
XM_025060740.1	XP_024916508.1	WASH complex subunit 2-like isoform X3
XM_008321891.3	XP_008320113.1	WASH complex subunit 2-like isoform X1
XM_008321893.2	XP_008320115.1	WASH complex subunit FAM21-like isoform X2
XM_008321891.2	XP_008320113.1	WASH complex subunit FAM21-like isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC103387313 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC103387313 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCy195661	XM_025060740.1 Latest version!	Cynoglossus semilaevis WASH complex subunit 2-like (LOC103387313), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OCy39598	XM_008321891.3 Latest version!	Cynoglossus semilaevis WASH complex subunit 2-like (LOC103387313), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCy39599	XM_008321893.3 Latest version!	Cynoglossus semilaevis WASH complex subunit 2-like (LOC103387313), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCy39600	XM_017037518.1 Latest version!	Cynoglossus semilaevis WASH complex subunit FAM21-like (LOC103387313), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
View Old Accession Versions >>
OCy39599	XM_008321893.2	Cynoglossus semilaevis WASH complex subunit FAM21-like (LOC103387313), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCy39598	XM_008321891.2	Cynoglossus semilaevis WASH complex subunit FAM21-like (LOC103387313), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC103387313 ( XM_025060740.1 ) cDNA ORF clone, Cynoglossus semilaevis(tongue sole) -> XP_024916508.1 Cynoglossus semilaevis WASH complex subunit 2-like (LOC103387313), transcript variant X3, mRNA.

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CloneID	OCy195661
Clone ID Related Accession (Same CDS sequence)	XM_025060740.1
Accession Version	XM_025060740.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-15
Organism	Cynoglossus semilaevis(tongue sole)
Product	WASH complex subunit 2-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024318.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cynoglossus semilaevis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGTT TTCCGGGGGG AATAGCAAAT GGTCCCAGCC AGAGTGAACA CATGGAGAAA 
GATGCCCGGG TATGGGAGAG ACCCTGGACG TTGGAAGAAA TGCGGCAGAG TAGTGCCAGC 
TGGTCCTTGG CAGCAGATTC AGGGCTCTTC CTGTTTCTGC AAGACTTCTC TCACAAAATG 
CTGTCAAAGA CGCATGAGAT TGAAAAACAA TTGGATGGCC TCATCCGTGA CACCAAGGCT 
ACAGACAGCT GCCTGCACTC TGTGTTCAGT GACTTCCTCA TGCTCTCCAA TACACAGTTT 
ATAGAAAATA GAGTATGTGA TGAAGAAGTC GAAGAACCAC TGCCTAAAAC TGATGCTGTG 
GAGAAACAAC CGGAACGGAC CCGGGAGCAA AAGGAGGCGG AGCTGATCCC TAAGATGCAG 
GAAGCTGTAA ATTACGGTCT ACGAGTGCTG GAGTCGGCGT TTGAGCATCT GGACATCAAG 
GCAGGGAACT CGGATTCAGA GGATGAGGAG GTCACGGGCA GAGTAGAGGC CATCCTGGAA 
CCTAAGGATC TTTATGTGGA CAGACCACTT CCCTATCTGA TTGGTTCCCA GGCGTTCATG 
GAACAGGATG ATGTTGGACT TGGCGACCTC TCCAGTGATG AAATGTCAGT AGACAGTGAC 
AGAGACAGCG TGATTGAAAG TGACGACGGC AAAGAAGCCA CACATTCGGA TGAAGATTTT 
ATTCAAGAAG GCCAGGTTCA CAACATCTTC AAAAAGAAAT CATCTATGTT GAGCTTCGAA 
GATGACGACG AGGAAGAGGA AGACGAGGAT TCGGACATAT TTGGAGAATC AGACAAGGAT 
GATGACGACC ACGACCCGAA GAACTCGGGT CCAATATCAT TTGCTGATGA GCTGGCAGCT 
CGGATCAGAG GTGAACCAGT TCATAAACAG GAGGGAGATC GTGCCTCATT GTCATCTAAG 
AAGAAAAGTA AAGGCAGAAA AGAAGCAAAG CCCTCAAAGA CCCAGGCTGA AGATGACAGT 
GACGACATGT TCAAACCACC CAAGATGGAT GAGATCGACG ATGACGATGA TGACGATGAC 
TTTTCCCCGT TTGGAGGAAG TAGCCGCCTC TTTAGTGGGG GGAAGGGCCT GTTTGATGAT 
GACAACGACG AGCGGGACCT TTTCGATGAT GCACCAAAAC CTTTTGTACC AGAAGAAAAG 
AAACCTGTAA AAGAAAGTGT GAAAGTCACA GCCACAACAG CAGAACCTAG GAAATCTGCA 
AAGAAGATTC CAACAGGTGG CGTTTCAATA TTTCCAGCCA ACAGCCTCTT CAGTCCAGGG 
AATGATTCTG ATTCTGTTAG AAGCAAGGAG AACGGAACTC CGAATTCCAA GGTCAGCGCT 
CCTCCAAAAG TTGAAAAAAA GACTGCTGTT GGTGGTGGAG GACTCTTTGA TGAGGAGGAT 
GAGGACGACG ACTTCTTTGG TGGAAAAAGT CTACAAAAGT CTGAATCTGT TGTAAAGGAG 
AAACCTAAAT CCAAGACAAC CGTTGACCTC TTTGATGAAG ATGATGAGGA TGATGGCAAT 
ATATTCGGTG ATAAATTTAA GTCAACAACT CCAGTTCAGG TCAAGAAGGA GGTTGTTGCA 
GAACCAGTTA AACCGCCTGA GAAGAAGATG CCAGCTGGGG CCATCTCCAT GTTTGGACCT 
GGAACTAAAA GTCTCCTCAG CGAAGGCCTG AAAAAGCGTC GACAATCGAC CAGTGAGGAG 
TCGGAAAAAT CTGAGGAGAA CGGTGCTTCT CCAGATGTTA GGAAAGCTGC TCCAAAACCA 
TCCCCGAAAC CCCAGACCAG GGGTCTTTTC TCTGATGATG AAGATGCACA AGCTTTTCCA 
ACCATCCCAA GAAGTCAATC GAAGCCTGAA CCTACAAACC AGAGCAACAC CAACAAAGCT 
CCTGTGTTCT TGTTTGATGA CGATGAAGAA GAGGATCAGT GGTTAGGTGC TACAAAAACA 
TCAGAGACCA ACTCAGGAGG AATGAAGGCC AGTGCTAGCG CCCCCTCTGG TCTCACTAGT 
ACCAAAACAA CACCCAGCAG CAGCCTGTTT GAGGAGGAAG ATGATAATGA ACTGTTTGCA 
CAAACAAAAG AATCAAGTAC TCAGAAAAAC CCTCAGCCAG TTGCACTACT GTTTGAAGAT 
AATGGTGGTG ATGATGAAGA TGACGAAGAT CAAGGAACCC TTTTTAATGT CAACACAAAT 
ACTGCACTAC CAACTACTAA AACACTAGCC TCAGCATCCA AGTCTTCCTC ATCCCAGATG 
CCTAAATCAG ACACAGTAGA ACATGTACCC ACAGAAAACC AGTCTTTGCA GCAGGAGAAG 
CTGACTCCAC ATACTTCAAA GTCTCCAGAG ATGCCTGTGG GAGAAAGTGG AGAAAGTAAA 
AAGAAGCCTG CTAGGGCTGT TAGTCTCTTC GGAGGCATTA ATGTTCTTGA AAAGCAGACA 
AAAACCCTAA TACAGGATGA AGAAGAAGAA GAAGATGATG ACTTCCCTTT GAAGCCCAGC 
CCTCCCCCAT TTGTCATGGA GGAGAAGGAA GAAAAATCTA GATCAGGGGC TTTTGGTCTG 
TTTGAAGATG AAGACGACGA CGAGTGGAAT GATTCTATTT TCACACCTGG TAAACCCACC 
AGCAAAACAA CACCCAAGGA AGAAGAAGAG GAGGAGGAGG AGGAGGCGGC GGAGGCGGAG 
GAGAAAGAGG AGGTGGAGCC AGCACAGACA AAGAGCACGG GTGTTCTCCA GGACGAGGAG 
TTGTTCAGCC ACAGTCATGA GAAAGACAAT GACCTCGACG CCGGTCTCTT TGCCACTTCA 
GGGAGAGCTG AAAGCCCACC AAAGGCAGCA GCACATAATT TGTTTGCAGA AGACGATGAA 
GATGACCTTT TCAGCTCCCC CAAGCCAAAA GTTCTTCCAA AAATTGCAGA AAAGCCAAGT 
AAACCTGCTG ACAAAGCACC GCTGGCCAGT CCAGAACCTG TCTCAGAGAC TGAGAAACCT 
GCACCAAGTC CTGTAAAACC TAAAGCTTCC TCATCAAGGA TTGGAAAACT TCAAGCAAGT 
CTCAAGATCA ATCCTGCTGC TATGCTTCCT GGTGCTGTTC CCACTTTGCC AGGTGCCATC 
AGTGTCTTCC CTGGCATGGA TCCCAGCTCC TCCTCCGGTG TGTCAAGCTC CAGCCTGAGC 
CCCAGCCCTG TGACAACTCC TGTAGGGGCT CAGACAGACA GTGAGGGAGT GACCTTTGAC 
ACCCCAGTCC AGGTCACGAC ACTGCAGAGT GTACAGAAAA GTCGCACCAA AGGTGCTGGT 
CAGCGCAGAC CCCAGACCAG AGCTGCAAGA CAGAAAGCTG TACAAAGATC AGTCGACGAC 
AGAGACAATT TCACAAGTAC AGCTGCGTCA GGTCCAAACC CCAATCTGTC TGATTCAGAG 
TCACGTCTAC CAGAGAGAGC CAGCCAGACG AGTAGCAGTT CAACGATTTC CACCTCTTCC 
ACGCCCACAG TCTTGGCCGC CTCCCCACCT TCTCAGCACA CTGTCCTGAA AGGATCCCCC 
AGACCTTCTG TTTTGTCATT GCCAGAGTCG AATGATGCTC TAAAGAAAGA GTCCAGTAAA 
CCCAGTGGTA AAGTGTTGGC ATACTCTGAT GTGGATGACA TTTTTGCCTC CGACGTTCTG 
TTTGAGGCCA CGTCGGTGAC AAACACACCC CCCACCGTAC TGTCAACCGC GAAGACACCA 
CCTGTAGGCA GCGGCGTGTT GGCTGCTAAA AAAGACAAAG ACAAAAGCAC ATTCCAGTCT 
ATTTTTGATG ACGATGTGGA TGATTTGTTT CAACCAGCCA AACCGAGGTC GACGGCCAAG 
AAAGCCAAGC AGTCGAAATT CTTGGAAGAC GACGATGATG ACGAGGACAT ATTCGGTGTC 
AGCAACAGTT CCACTCCGTC ATCGACCAGT AGTAAAGAAA TGAAGAATGG TAGTAGTTTT 
TCCAAGCAGG AAATTTTCCA GGACGAAGTA ACTACTGTGC CTAAAGTTCA AAAGAAGCAC 
AAAGAGAAGC CTTTAGATTT CAGCTTGTTC GATGATGACA CAGACATTTT TGCTGATCTG 
ACAGACACTT TCAAACCAAA GCAGAAACTC AAGACAAAGC CAGAGGCCAA GTCGATATTT 
GACGATGACA TGGATGACAT CTTCTCATCG AGCAAAGTGA AGAAAGTTTC AAAGGCTTCA 
AATAAATCTA AGAAAACGAT ACCCGCCCAG GACACGACTG CAGCGACAGA TTCCAGCAAC 
ATATTTGACG ATCCACTAAA CGCCCTTGGT GGGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024916508.1
CDS	156..4454
Translation	MSGFPGGIANGPSQSEHMEKDARVWERPWTLEEMRQSSASWSLAADSGLFLFLQDFSHKMLSKTHEIEKQLDGLIRDTKATDSCLHSVFSDFLMLSNTQFIENRVCDEEVEEPLPKTDAVEKQPERTREQKEAELIPKMQEAVNYGLRVLESAFEHLDIKAGNSDSEDEEVTGRVEAILEPKDLYVDRPLPYLIGSQAFMEQDDVGLGDLSSDEMSVDSDRDSVIESDDGKEATHSDEDFIQEGQVHNIFKKKSSMLSFEDDDEEEEDEDSDIFGESDKDDDDHDPKNSGPISFADELAARIRGEPVHKQEGDRASLSSKKKSKGRKEAKPSKTQAEDDSDDMFKPPKMDEIDDDDDDDDFSPFGGSSRLFSGGKGLFDDDNDERDLFDDAPKPFVPEEKKPVKESVKVTATTAEPRKSAKKIPTGGVSIFPANSLFSPGNDSDSVRSKENGTPNSKVSAPPKVEKKTAVGGGGLFDEEDEDDDFFGGKSLQKSESVVKEKPKSKTTVDLFDEDDEDDGNIFGDKFKSTTPVQVKKEVVAEPVKPPEKKMPAGAISMFGPGTKSLLSEGLKKRRQSTSEESEKSEENGASPDVRKAAPKPSPKPQTRGLFSDDEDAQAFPTIPRSQSKPEPTNQSNTNKAPVFLFDDDEEEDQWLGATKTSETNSGGMKASASAPSGLTSTKTTPSSSLFEEEDDNELFAQTKESSTQKNPQPVALLFEDNGGDDEDDEDQGTLFNVNTNTALPTTKTLASASKSSSSQMPKSDTVEHVPTENQSLQQEKLTPHTSKSPEMPVGESGESKKKPARAVSLFGGINVLEKQTKTLIQDEEEEEDDDFPLKPSPPPFVMEEKEEKSRSGAFGLFEDEDDDEWNDSIFTPGKPTSKTTPKEEEEEEEEEAAEAEEKEEVEPAQTKSTGVLQDEELFSHSHEKDNDLDAGLFATSGRAESPPKAAAHNLFAEDDEDDLFSSPKPKVLPKIAEKPSKPADKAPLASPEPVSETEKPAPSPVKPKASSSRIGKLQASLKINPAAMLPGAVPTLPGAISVFPGMDPSSSSGVSSSSLSPSPVTTPVGAQTDSEGVTFDTPVQVTTLQSVQKSRTKGAGQRRPQTRAARQKAVQRSVDDRDNFTSTAASGPNPNLSDSESRLPERASQTSSSSTISTSSTPTVLAASPPSQHTVLKGSPRPSVLSLPESNDALKKESSKPSGKVLAYSDVDDIFASDVLFEATSVTNTPPTVLSTAKTPPVGSGVLAAKKDKDKSTFQSIFDDDVDDLFQPAKPRSTAKKAKQSKFLEDDDDDEDIFGVSNSSTPSSTSSKEMKNGSSFSKQEIFQDEVTTVPKVQKKHKEKPLDFSLFDDDTDIFADLTDTFKPKQKLKTKPEAKSIFDDDMDDIFSSSKVKKVSKASNKSKKTIPAQDTTAATDSSNIFDDPLNALGGT

Target ORF information:

RefSeq Version	XM_025060740.1
Organism	Cynoglossus semilaevis(tongue sole)
Definition	Cynoglossus semilaevis WASH complex subunit 2-like (LOC103387313), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025060740.1

ORF Insert Sequence:

ATGAGCGGTT TTCCGGGGGG AATAGCAAAT GGTCCCAGCC AGAGTGAACA CATGGAGAAA 
GATGCCCGGG TATGGGAGAG ACCCTGGACG TTGGAAGAAA TGCGGCAGAG TAGTGCCAGC 
TGGTCCTTGG CAGCAGATTC AGGGCTCTTC CTGTTTCTGC AAGACTTCTC TCACAAAATG 
CTGTCAAAGA CGCATGAGAT TGAAAAACAA TTGGATGGCC TCATCCGTGA CACCAAGGCT 
ACAGACAGCT GCCTGCACTC TGTGTTCAGT GACTTCCTCA TGCTCTCCAA TACACAGTTT 
ATAGAAAATA GAGTATGTGA TGAAGAAGTC GAAGAACCAC TGCCTAAAAC TGATGCTGTG 
GAGAAACAAC CGGAACGGAC CCGGGAGCAA AAGGAGGCGG AGCTGATCCC TAAGATGCAG 
GAAGCTGTAA ATTACGGTCT ACGAGTGCTG GAGTCGGCGT TTGAGCATCT GGACATCAAG 
GCAGGGAACT CGGATTCAGA GGATGAGGAG GTCACGGGCA GAGTAGAGGC CATCCTGGAA 
CCTAAGGATC TTTATGTGGA CAGACCACTT CCCTATCTGA TTGGTTCCCA GGCGTTCATG 
GAACAGGATG ATGTTGGACT TGGCGACCTC TCCAGTGATG AAATGTCAGT AGACAGTGAC 
AGAGACAGCG TGATTGAAAG TGACGACGGC AAAGAAGCCA CACATTCGGA TGAAGATTTT 
ATTCAAGAAG GCCAGGTTCA CAACATCTTC AAAAAGAAAT CATCTATGTT GAGCTTCGAA 
GATGACGACG AGGAAGAGGA AGACGAGGAT TCGGACATAT TTGGAGAATC AGACAAGGAT 
GATGACGACC ACGACCCGAA GAACTCGGGT CCAATATCAT TTGCTGATGA GCTGGCAGCT 
CGGATCAGAG GTGAACCAGT TCATAAACAG GAGGGAGATC GTGCCTCATT GTCATCTAAG 
AAGAAAAGTA AAGGCAGAAA AGAAGCAAAG CCCTCAAAGA CCCAGGCTGA AGATGACAGT 
GACGACATGT TCAAACCACC CAAGATGGAT GAGATCGACG ATGACGATGA TGACGATGAC 
TTTTCCCCGT TTGGAGGAAG TAGCCGCCTC TTTAGTGGGG GGAAGGGCCT GTTTGATGAT 
GACAACGACG AGCGGGACCT TTTCGATGAT GCACCAAAAC CTTTTGTACC AGAAGAAAAG 
AAACCTGTAA AAGAAAGTGT GAAAGTCACA GCCACAACAG CAGAACCTAG GAAATCTGCA 
AAGAAGATTC CAACAGGTGG CGTTTCAATA TTTCCAGCCA ACAGCCTCTT CAGTCCAGGG 
AATGATTCTG ATTCTGTTAG AAGCAAGGAG AACGGAACTC CGAATTCCAA GGTCAGCGCT 
CCTCCAAAAG TTGAAAAAAA GACTGCTGTT GGTGGTGGAG GACTCTTTGA TGAGGAGGAT 
GAGGACGACG ACTTCTTTGG TGGAAAAAGT CTACAAAAGT CTGAATCTGT TGTAAAGGAG 
AAACCTAAAT CCAAGACAAC CGTTGACCTC TTTGATGAAG ATGATGAGGA TGATGGCAAT 
ATATTCGGTG ATAAATTTAA GTCAACAACT CCAGTTCAGG TCAAGAAGGA GGTTGTTGCA 
GAACCAGTTA AACCGCCTGA GAAGAAGATG CCAGCTGGGG CCATCTCCAT GTTTGGACCT 
GGAACTAAAA GTCTCCTCAG CGAAGGCCTG AAAAAGCGTC GACAATCGAC CAGTGAGGAG 
TCGGAAAAAT CTGAGGAGAA CGGTGCTTCT CCAGATGTTA GGAAAGCTGC TCCAAAACCA 
TCCCCGAAAC CCCAGACCAG GGGTCTTTTC TCTGATGATG AAGATGCACA AGCTTTTCCA 
ACCATCCCAA GAAGTCAATC GAAGCCTGAA CCTACAAACC AGAGCAACAC CAACAAAGCT 
CCTGTGTTCT TGTTTGATGA CGATGAAGAA GAGGATCAGT GGTTAGGTGC TACAAAAACA 
TCAGAGACCA ACTCAGGAGG AATGAAGGCC AGTGCTAGCG CCCCCTCTGG TCTCACTAGT 
ACCAAAACAA CACCCAGCAG CAGCCTGTTT GAGGAGGAAG ATGATAATGA ACTGTTTGCA 
CAAACAAAAG AATCAAGTAC TCAGAAAAAC CCTCAGCCAG TTGCACTACT GTTTGAAGAT 
AATGGTGGTG ATGATGAAGA TGACGAAGAT CAAGGAACCC TTTTTAATGT CAACACAAAT 
ACTGCACTAC CAACTACTAA AACACTAGCC TCAGCATCCA AGTCTTCCTC ATCCCAGATG 
CCTAAATCAG ACACAGTAGA ACATGTACCC ACAGAAAACC AGTCTTTGCA GCAGGAGAAG 
CTGACTCCAC ATACTTCAAA GTCTCCAGAG ATGCCTGTGG GAGAAAGTGG AGAAAGTAAA 
AAGAAGCCTG CTAGGGCTGT TAGTCTCTTC GGAGGCATTA ATGTTCTTGA AAAGCAGACA 
AAAACCCTAA TACAGGATGA AGAAGAAGAA GAAGATGATG ACTTCCCTTT GAAGCCCAGC 
CCTCCCCCAT TTGTCATGGA GGAGAAGGAA GAAAAATCTA GATCAGGGGC TTTTGGTCTG 
TTTGAAGATG AAGACGACGA CGAGTGGAAT GATTCTATTT TCACACCTGG TAAACCCACC 
AGCAAAACAA CACCCAAGGA AGAAGAAGAG GAGGAGGAGG AGGAGGCGGC GGAGGCGGAG 
GAGAAAGAGG AGGTGGAGCC AGCACAGACA AAGAGCACGG GTGTTCTCCA GGACGAGGAG 
TTGTTCAGCC ACAGTCATGA GAAAGACAAT GACCTCGACG CCGGTCTCTT TGCCACTTCA 
GGGAGAGCTG AAAGCCCACC AAAGGCAGCA GCACATAATT TGTTTGCAGA AGACGATGAA 
GATGACCTTT TCAGCTCCCC CAAGCCAAAA GTTCTTCCAA AAATTGCAGA AAAGCCAAGT 
AAACCTGCTG ACAAAGCACC GCTGGCCAGT CCAGAACCTG TCTCAGAGAC TGAGAAACCT 
GCACCAAGTC CTGTAAAACC TAAAGCTTCC TCATCAAGGA TTGGAAAACT TCAAGCAAGT 
CTCAAGATCA ATCCTGCTGC TATGCTTCCT GGTGCTGTTC CCACTTTGCC AGGTGCCATC 
AGTGTCTTCC CTGGCATGGA TCCCAGCTCC TCCTCCGGTG TGTCAAGCTC CAGCCTGAGC 
CCCAGCCCTG TGACAACTCC TGTAGGGGCT CAGACAGACA GTGAGGGAGT GACCTTTGAC 
ACCCCAGTCC AGGTCACGAC ACTGCAGAGT GTACAGAAAA GTCGCACCAA AGGTGCTGGT 
CAGCGCAGAC CCCAGACCAG AGCTGCAAGA CAGAAAGCTG TACAAAGATC AGTCGACGAC 
AGAGACAATT TCACAAGTAC AGCTGCGTCA GGTCCAAACC CCAATCTGTC TGATTCAGAG 
TCACGTCTAC CAGAGAGAGC CAGCCAGACG AGTAGCAGTT CAACGATTTC CACCTCTTCC 
ACGCCCACAG TCTTGGCCGC CTCCCCACCT TCTCAGCACA CTGTCCTGAA AGGATCCCCC 
AGACCTTCTG TTTTGTCATT GCCAGAGTCG AATGATGCTC TAAAGAAAGA GTCCAGTAAA 
CCCAGTGGTA AAGTGTTGGC ATACTCTGAT GTGGATGACA TTTTTGCCTC CGACGTTCTG 
TTTGAGGCCA CGTCGGTGAC AAACACACCC CCCACCGTAC TGTCAACCGC GAAGACACCA 
CCTGTAGGCA GCGGCGTGTT GGCTGCTAAA AAAGACAAAG ACAAAAGCAC ATTCCAGTCT 
ATTTTTGATG ACGATGTGGA TGATTTGTTT CAACCAGCCA AACCGAGGTC GACGGCCAAG 
AAAGCCAAGC AGTCGAAATT CTTGGAAGAC GACGATGATG ACGAGGACAT ATTCGGTGTC 
AGCAACAGTT CCACTCCGTC ATCGACCAGT AGTAAAGAAA TGAAGAATGG TAGTAGTTTT 
TCCAAGCAGG AAATTTTCCA GGACGAAGTA ACTACTGTGC CTAAAGTTCA AAAGAAGCAC 
AAAGAGAAGC CTTTAGATTT CAGCTTGTTC GATGATGACA CAGACATTTT TGCTGATCTG 
ACAGACACTT TCAAACCAAA GCAGAAACTC AAGACAAAGC CAGAGGCCAA GTCGATATTT 
GACGATGACA TGGATGACAT CTTCTCATCG AGCAAAGTGA AGAAAGTTTC AAAGGCTTCA 
AATAAATCTA AGAAAACGAT ACCCGCCCAG GACACGACTG CAGCGACAGA TTCCAGCAAC 
ATATTTGACG ATCCACTAAA CGCCCTTGGT GGGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103387313 ( XM_008321891.3 ) cDNA ORF clone, Cynoglossus semilaevis(tongue sole) -> XP_008320113.1 Cynoglossus semilaevis WASH complex subunit 2-like (LOC103387313), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCy39598
Clone ID Related Accession (Same CDS sequence)	XM_008321891.3 , XM_008321891.2
Accession Version	XM_008321891.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-15
Organism	Cynoglossus semilaevis(tongue sole)
Product	WASH complex subunit 2-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024318.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 16, 2018 this sequence version replaced XM_008321891.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cynoglossus semilaevis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGTT TTCCGGGGGG AATAGCAAAT GGTCCCAGCC AGAGTGAACA CATGGAGAAA 
GATGCCCGGG TATGGGAGAG ACCCTGGACG TTGGAAGAAA TGCGGCAGAG TAGTGCCAGC 
TGGTCCTTGG CAGCAGATTC AGGGCTCTTC CTGTTTCTGC AAGACTTCTC TCACAAAATG 
CTGTCAAAGA CGCATGAGAT TGAAAAACAA TTGGATGGCC TCATCCGTGA CACCAAGGCT 
ACAGACAGCT GCCTGCACTC TGTGTTCAGT GACTTCCTCA TGCTCTCCAA TACACAGTTT 
ATAGAAAATA GAGTATGTGA TGAAGAAGTC GAAGAACCAC TGCCTAAAAC TGATGCTGTG 
GAGAAACAAC CGGAACGGAC CCGGGAGCAA AAGGAGGCGG AGCTGATCCC TAAGATGCAG 
GAAGCTGTAA ATTACGGTCT ACGAGTGCTG GAGTCGGCGT TTGAGCATCT GGACATCAAG 
GCAGGGAACT CGGATTCAGA GGATGAGGAG GTCACGGGCA GAGTAGAGGC CATCCTGGAA 
CCTAAGGATC TTTATGTGGA CAGACCACTT CCCTATCTGA TTGGTTCCCA GGCGTTCATG 
GAACAGGATG ATGTTGGACT TGGCGACCTC TCCAGTGATG AAATGTCAGT AGACAGTGAC 
AGAGACAGCG TGATTGAAAG TGACGACGGC AAAGAAGCCA CACATTCGGA TGAAGATTTT 
ATTCAAGAAG GCCAGGTTCA CAACATCTTC AAAAAGAAAT CATCTATGTT GAGCTTCGAA 
GATGACGACG AGGAAGAGGA AGACGAGGAT TCGGACATAT TTGGAGAATC AGACAAGGAT 
GATGACGACC ACGACCCGAA GAACTCGGGT CCAATATCAT TTGCTGATGA GCTGGCAGCT 
CGGATCAGAG GTGAACCAGT TCATAAACAG GAGGGAGATC GTGCCTCATT GTCATCTAAG 
AAGAAAAGTA AAGGCAGAAA AGAAGCAAAG CCCTCAAAGA CCCAGGCTGA AGATGACAGT 
GACGACATGT TCAAACCACC CAAGATGGAT GAGATCGACG ATGACGATGA TGACGATGAC 
TTTTCCCCGT TTGGAGGAAG TAGCCGCCTC TTTAGTGGGG GGAAGGGCCT GTTTGATGAT 
GACAACGACG AGCGGGACCT TTTCGATGAT GCACCAAAAC CTTTTGTACC AGAAGAAAAG 
AAACCTGTAA AAGAAAGTGT GAAAGTCACA GCCACAACAG CAGAACCTAG GAAATCTGCA 
AAGAAGATTC CAACAGGTGG CGTTTCAATA TTTCCAGCCA ACAGCCTCTT CAGTCCAGGG 
AATGATTCTG ATTCTGTTAG AAGCAAGGAG AACGGAACTC CGAATTCCAA GGTCAGCGCT 
CCTCCAAAAG TTGAAAAAAA GACTGCTGTT GGTGGTGGAG GACTCTTTGA TGAGGAGGAT 
GAGGACGACG ACTTCTTTGG TGGAAAAAGT CTACAAAAGT CTGAATCTGT TGTAAAGGAG 
AAACCTAAAT CCAAGACAAC CGTTGACCTC TTTGATGAAG ATGATGAGGA TGATGGCAAT 
ATATTCGGTG ATAAATTTAA GTCAACAACT CCAGTTCAGG TCAAGAAGGA GGTTGTTGCA 
GAACCAGTTA AACCGCCTGA GAAGAAGATG CCAGCTGGGG CCATCTCCAT GTTTGGACCT 
GGAACTAAAA GTCTCCTCAG CGAAGGCCTG AAAAAGCGTC GACAATCGAC CAGTGAGGAG 
TCGGAAAAAT CTGAGGAGAA CGGTGCTTCT CCAGATGTTA GGAAAGCTGC TCCAAAACCA 
TCCCCGAAAC CCCAGACCAG GGGTCTTTTC TCTGATGATG AAGATGCACA AGCTTTTCCA 
ACCATCCCAA GAAGTCAATC GAAGCCTGAA CCTACAAACC AGAGCAACAC CAACAAAGCT 
CCTGTGTTCT TGTTTGATGA CGATGAAGAA GAGGATCTCT TTGCTTCTGC GGTTAAATCT 
AAACCAAAAG CTAGTTCATC TAAAACCTCA CCCATGCAAA CCAGCAGTGC AGTCTCTAGC 
TCCCTCTTCA GTGATGATGA GGATCAGTGG TTAGGTGCTA CAAAAACATC AGAGACCAAC 
TCAGGAGGAA TGAAGGCCAG TGCTAGCGCC CCCTCTGGTC TCACTAGTAC CAAAACAACA 
CCCAGCAGCA GCCTGTTTGA GGAGGAAGAT GATAATGAAC TGTTTGCACA AACAAAAGAA 
TCAAGTACTC AGAAAAACCC TCAGCCAGTT GCACTACTGT TTGAAGATAA TGGTGGTGAT 
GATGAAGATG ACGAAGATCA AGGAACCCTT TTTAATGTCA ACACAAATAC TGCACTACCA 
ACTACTAAAA CACTAGCCTC AGCATCCAAG TCTTCCTCAT CCCAGATGCC TAAATCAGAC 
ACAGTAGAAC ATGTACCCAC AGAAAACCAG TCTTTGCAGC AGGAGAAGCT GACTCCACAT 
ACTTCAAAGT CTCCAGAGAT GCCTGTGGGA GAAAGTGGAG AAAGTAAAAA GAAGCCTGCT 
AGGGCTGTTA GTCTCTTCGG AGGCATTAAT GTTCTTGAAA AGCAGACAAA AACCCTAATA 
CAGGATGAAG AAGAAGAAGA AGATGATGAC TTCCCTTTGA AGCCCAGCCC TCCCCCATTT 
GTCATGGAGG AGAAGGAAGA AAAATCTAGA TCAGGGGCTT TTGGTCTGTT TGAAGATGAA 
GACGACGACG AGTGGAATGA TTCTATTTTC ACACCTGGTA AACCCACCAG CAAAACAACA 
CCCAAGGAAG AAGAAGAGGA GGAGGAGGAG GAGGCGGCGG AGGCGGAGGA GAAAGAGGAG 
GTGGAGCCAG CACAGACAAA GAGCACGGGT GTTCTCCAGG ACGAGGAGTT GTTCAGCCAC 
AGTCATGAGA AAGACAATGA CCTCGACGCC GGTCTCTTTG CCACTTCAGG GAGAGCTGAA 
AGCCCACCAA AGGCAGCAGC ACATAATTTG TTTGCAGAAG ACGATGAAGA TGACCTTTTC 
AGCTCCCCCA AGCCAAAAGT TCTTCCAAAA ATTGCAGAAA AGCCAAGTAA ACCTGCTGAC 
AAAGCACCGC TGGCCAGTCC AGAACCTGTC TCAGAGACTG AGAAACCTGC ACCAAGTCCT 
GTAAAACCTA AAGCTTCCTC ATCAAGGATT GGAAAACTTC AAGCAAGTCT CAAGATCAAT 
CCTGCTGCTA TGCTTCCTGG TGCTGTTCCC ACTTTGCCAG GTGCCATCAG TGTCTTCCCT 
GGCATGGATC CCAGCTCCTC CTCCGGTGTG TCAAGCTCCA GCCTGAGCCC CAGCCCTGTG 
ACAACTCCTG TAGGGGCTCA GACAGACAGT GAGGGAGTGA CCTTTGACAC CCCAGTCCAG 
GTCACGACAC TGCAGAGTGT ACAGAAAAGT CGCACCAAAG GTGCTGGTCA GCGCAGACCC 
CAGACCAGAG CTGCAAGACA GAAAGCTGTA CAAAGATCAG TCGACGACAG AGACAATTTC 
ACAAGTACAG CTGCGTCAGG TCCAAACCCC AATCTGTCTG ATTCAGAGTC ACGTCTACCA 
GAGAGAGCCA GCCAGACGAG TAGCAGTTCA ACGATTTCCA CCTCTTCCAC GCCCACAGTC 
TTGGCCGCCT CCCCACCTTC TCAGCACACT GTCCTGAAAG GATCCCCCAG ACCTTCTGTT 
TTGTCATTGC CAGAGTCGAA TGATGCTCTA AAGAAAGAGT CCAGTAAACC CAGTGGTAAA 
GTGTTGGCAT ACTCTGATGT GGATGACATT TTTGCCTCCG ACGTTCTGTT TGAGGCCACG 
TCGGTGACAA ACACACCCCC CACCGTACTG TCAACCGCGA AGACACCACC TGTAGGCAGC 
GGCGTGTTGG CTGCTAAAAA AGACAAAGAC AAAAGCACAT TCCAGTCTAT TTTTGATGAC 
GATGTGGATG ATTTGTTTCA ACCAGCCAAA CCGAGGTCGA CGGCCAAGAA AGCCAAGCAG 
TCGAAATTCT TGGAAGACGA CGATGATGAC GAGGACATAT TCGGTGTCAG CAACAGTTCC 
ACTCCGTCAT CGACCAGTAG TAAAGAAATG AAGAATGGTA GTAGTTTTTC CAAGCAGGAA 
ATTTTCCAGG ACGAAGTAAC TACTGTGCCT AAAGTTCAAA AGAAGCACAA AGAGAAGCCT 
TTAGATTTCA GCTTGTTCGA TGATGACACA GACATTTTTG CTGATCTGAC AGACACTTTC 
AAACCAAAGC AGAAACTCAA GACAAAGCCA GAGGCCAAGT CGATATTTGA CGATGACATG 
GATGACATCT TCTCATCGAG CAAAGTGAAG AAAGTTTCAA AGGCTTCAAA TAAATCTAAG 
AAAACGATAC CCGCCCAGGA CACGACTGCA GCGACAGATT CCAGCAACAT ATTTGACGAT 
CCACTAAACG CCCTTGGTGG GACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008320113.1
CDS	156..4562
Translation	MSGFPGGIANGPSQSEHMEKDARVWERPWTLEEMRQSSASWSLAADSGLFLFLQDFSHKMLSKTHEIEKQLDGLIRDTKATDSCLHSVFSDFLMLSNTQFIENRVCDEEVEEPLPKTDAVEKQPERTREQKEAELIPKMQEAVNYGLRVLESAFEHLDIKAGNSDSEDEEVTGRVEAILEPKDLYVDRPLPYLIGSQAFMEQDDVGLGDLSSDEMSVDSDRDSVIESDDGKEATHSDEDFIQEGQVHNIFKKKSSMLSFEDDDEEEEDEDSDIFGESDKDDDDHDPKNSGPISFADELAARIRGEPVHKQEGDRASLSSKKKSKGRKEAKPSKTQAEDDSDDMFKPPKMDEIDDDDDDDDFSPFGGSSRLFSGGKGLFDDDNDERDLFDDAPKPFVPEEKKPVKESVKVTATTAEPRKSAKKIPTGGVSIFPANSLFSPGNDSDSVRSKENGTPNSKVSAPPKVEKKTAVGGGGLFDEEDEDDDFFGGKSLQKSESVVKEKPKSKTTVDLFDEDDEDDGNIFGDKFKSTTPVQVKKEVVAEPVKPPEKKMPAGAISMFGPGTKSLLSEGLKKRRQSTSEESEKSEENGASPDVRKAAPKPSPKPQTRGLFSDDEDAQAFPTIPRSQSKPEPTNQSNTNKAPVFLFDDDEEEDLFASAVKSKPKASSSKTSPMQTSSAVSSSLFSDDEDQWLGATKTSETNSGGMKASASAPSGLTSTKTTPSSSLFEEEDDNELFAQTKESSTQKNPQPVALLFEDNGGDDEDDEDQGTLFNVNTNTALPTTKTLASASKSSSSQMPKSDTVEHVPTENQSLQQEKLTPHTSKSPEMPVGESGESKKKPARAVSLFGGINVLEKQTKTLIQDEEEEEDDDFPLKPSPPPFVMEEKEEKSRSGAFGLFEDEDDDEWNDSIFTPGKPTSKTTPKEEEEEEEEEAAEAEEKEEVEPAQTKSTGVLQDEELFSHSHEKDNDLDAGLFATSGRAESPPKAAAHNLFAEDDEDDLFSSPKPKVLPKIAEKPSKPADKAPLASPEPVSETEKPAPSPVKPKASSSRIGKLQASLKINPAAMLPGAVPTLPGAISVFPGMDPSSSSGVSSSSLSPSPVTTPVGAQTDSEGVTFDTPVQVTTLQSVQKSRTKGAGQRRPQTRAARQKAVQRSVDDRDNFTSTAASGPNPNLSDSESRLPERASQTSSSSTISTSSTPTVLAASPPSQHTVLKGSPRPSVLSLPESNDALKKESSKPSGKVLAYSDVDDIFASDVLFEATSVTNTPPTVLSTAKTPPVGSGVLAAKKDKDKSTFQSIFDDDVDDLFQPAKPRSTAKKAKQSKFLEDDDDDEDIFGVSNSSTPSSTSSKEMKNGSSFSKQEIFQDEVTTVPKVQKKHKEKPLDFSLFDDDTDIFADLTDTFKPKQKLKTKPEAKSIFDDDMDDIFSSSKVKKVSKASNKSKKTIPAQDTTAATDSSNIFDDPLNALGGT

Target ORF information:

RefSeq Version	XM_008321891.3
Organism	Cynoglossus semilaevis(tongue sole)
Definition	Cynoglossus semilaevis WASH complex subunit 2-like (LOC103387313), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008321891.3

ORF Insert Sequence:

ATGAGCGGTT TTCCGGGGGG AATAGCAAAT GGTCCCAGCC AGAGTGAACA CATGGAGAAA 
GATGCCCGGG TATGGGAGAG ACCCTGGACG TTGGAAGAAA TGCGGCAGAG TAGTGCCAGC 
TGGTCCTTGG CAGCAGATTC AGGGCTCTTC CTGTTTCTGC AAGACTTCTC TCACAAAATG 
CTGTCAAAGA CGCATGAGAT TGAAAAACAA TTGGATGGCC TCATCCGTGA CACCAAGGCT 
ACAGACAGCT GCCTGCACTC TGTGTTCAGT GACTTCCTCA TGCTCTCCAA TACACAGTTT 
ATAGAAAATA GAGTATGTGA TGAAGAAGTC GAAGAACCAC TGCCTAAAAC TGATGCTGTG 
GAGAAACAAC CGGAACGGAC CCGGGAGCAA AAGGAGGCGG AGCTGATCCC TAAGATGCAG 
GAAGCTGTAA ATTACGGTCT ACGAGTGCTG GAGTCGGCGT TTGAGCATCT GGACATCAAG 
GCAGGGAACT CGGATTCAGA GGATGAGGAG GTCACGGGCA GAGTAGAGGC CATCCTGGAA 
CCTAAGGATC TTTATGTGGA CAGACCACTT CCCTATCTGA TTGGTTCCCA GGCGTTCATG 
GAACAGGATG ATGTTGGACT TGGCGACCTC TCCAGTGATG AAATGTCAGT AGACAGTGAC 
AGAGACAGCG TGATTGAAAG TGACGACGGC AAAGAAGCCA CACATTCGGA TGAAGATTTT 
ATTCAAGAAG GCCAGGTTCA CAACATCTTC AAAAAGAAAT CATCTATGTT GAGCTTCGAA 
GATGACGACG AGGAAGAGGA AGACGAGGAT TCGGACATAT TTGGAGAATC AGACAAGGAT 
GATGACGACC ACGACCCGAA GAACTCGGGT CCAATATCAT TTGCTGATGA GCTGGCAGCT 
CGGATCAGAG GTGAACCAGT TCATAAACAG GAGGGAGATC GTGCCTCATT GTCATCTAAG 
AAGAAAAGTA AAGGCAGAAA AGAAGCAAAG CCCTCAAAGA CCCAGGCTGA AGATGACAGT 
GACGACATGT TCAAACCACC CAAGATGGAT GAGATCGACG ATGACGATGA TGACGATGAC 
TTTTCCCCGT TTGGAGGAAG TAGCCGCCTC TTTAGTGGGG GGAAGGGCCT GTTTGATGAT 
GACAACGACG AGCGGGACCT TTTCGATGAT GCACCAAAAC CTTTTGTACC AGAAGAAAAG 
AAACCTGTAA AAGAAAGTGT GAAAGTCACA GCCACAACAG CAGAACCTAG GAAATCTGCA 
AAGAAGATTC CAACAGGTGG CGTTTCAATA TTTCCAGCCA ACAGCCTCTT CAGTCCAGGG 
AATGATTCTG ATTCTGTTAG AAGCAAGGAG AACGGAACTC CGAATTCCAA GGTCAGCGCT 
CCTCCAAAAG TTGAAAAAAA GACTGCTGTT GGTGGTGGAG GACTCTTTGA TGAGGAGGAT 
GAGGACGACG ACTTCTTTGG TGGAAAAAGT CTACAAAAGT CTGAATCTGT TGTAAAGGAG 
AAACCTAAAT CCAAGACAAC CGTTGACCTC TTTGATGAAG ATGATGAGGA TGATGGCAAT 
ATATTCGGTG ATAAATTTAA GTCAACAACT CCAGTTCAGG TCAAGAAGGA GGTTGTTGCA 
GAACCAGTTA AACCGCCTGA GAAGAAGATG CCAGCTGGGG CCATCTCCAT GTTTGGACCT 
GGAACTAAAA GTCTCCTCAG CGAAGGCCTG AAAAAGCGTC GACAATCGAC CAGTGAGGAG 
TCGGAAAAAT CTGAGGAGAA CGGTGCTTCT CCAGATGTTA GGAAAGCTGC TCCAAAACCA 
TCCCCGAAAC CCCAGACCAG GGGTCTTTTC TCTGATGATG AAGATGCACA AGCTTTTCCA 
ACCATCCCAA GAAGTCAATC GAAGCCTGAA CCTACAAACC AGAGCAACAC CAACAAAGCT 
CCTGTGTTCT TGTTTGATGA CGATGAAGAA GAGGATCTCT TTGCTTCTGC GGTTAAATCT 
AAACCAAAAG CTAGTTCATC TAAAACCTCA CCCATGCAAA CCAGCAGTGC AGTCTCTAGC 
TCCCTCTTCA GTGATGATGA GGATCAGTGG TTAGGTGCTA CAAAAACATC AGAGACCAAC 
TCAGGAGGAA TGAAGGCCAG TGCTAGCGCC CCCTCTGGTC TCACTAGTAC CAAAACAACA 
CCCAGCAGCA GCCTGTTTGA GGAGGAAGAT GATAATGAAC TGTTTGCACA AACAAAAGAA 
TCAAGTACTC AGAAAAACCC TCAGCCAGTT GCACTACTGT TTGAAGATAA TGGTGGTGAT 
GATGAAGATG ACGAAGATCA AGGAACCCTT TTTAATGTCA ACACAAATAC TGCACTACCA 
ACTACTAAAA CACTAGCCTC AGCATCCAAG TCTTCCTCAT CCCAGATGCC TAAATCAGAC 
ACAGTAGAAC ATGTACCCAC AGAAAACCAG TCTTTGCAGC AGGAGAAGCT GACTCCACAT 
ACTTCAAAGT CTCCAGAGAT GCCTGTGGGA GAAAGTGGAG AAAGTAAAAA GAAGCCTGCT 
AGGGCTGTTA GTCTCTTCGG AGGCATTAAT GTTCTTGAAA AGCAGACAAA AACCCTAATA 
CAGGATGAAG AAGAAGAAGA AGATGATGAC TTCCCTTTGA AGCCCAGCCC TCCCCCATTT 
GTCATGGAGG AGAAGGAAGA AAAATCTAGA TCAGGGGCTT TTGGTCTGTT TGAAGATGAA 
GACGACGACG AGTGGAATGA TTCTATTTTC ACACCTGGTA AACCCACCAG CAAAACAACA 
CCCAAGGAAG AAGAAGAGGA GGAGGAGGAG GAGGCGGCGG AGGCGGAGGA GAAAGAGGAG 
GTGGAGCCAG CACAGACAAA GAGCACGGGT GTTCTCCAGG ACGAGGAGTT GTTCAGCCAC 
AGTCATGAGA AAGACAATGA CCTCGACGCC GGTCTCTTTG CCACTTCAGG GAGAGCTGAA 
AGCCCACCAA AGGCAGCAGC ACATAATTTG TTTGCAGAAG ACGATGAAGA TGACCTTTTC 
AGCTCCCCCA AGCCAAAAGT TCTTCCAAAA ATTGCAGAAA AGCCAAGTAA ACCTGCTGAC 
AAAGCACCGC TGGCCAGTCC AGAACCTGTC TCAGAGACTG AGAAACCTGC ACCAAGTCCT 
GTAAAACCTA AAGCTTCCTC ATCAAGGATT GGAAAACTTC AAGCAAGTCT CAAGATCAAT 
CCTGCTGCTA TGCTTCCTGG TGCTGTTCCC ACTTTGCCAG GTGCCATCAG TGTCTTCCCT 
GGCATGGATC CCAGCTCCTC CTCCGGTGTG TCAAGCTCCA GCCTGAGCCC CAGCCCTGTG 
ACAACTCCTG TAGGGGCTCA GACAGACAGT GAGGGAGTGA CCTTTGACAC CCCAGTCCAG 
GTCACGACAC TGCAGAGTGT ACAGAAAAGT CGCACCAAAG GTGCTGGTCA GCGCAGACCC 
CAGACCAGAG CTGCAAGACA GAAAGCTGTA CAAAGATCAG TCGACGACAG AGACAATTTC 
ACAAGTACAG CTGCGTCAGG TCCAAACCCC AATCTGTCTG ATTCAGAGTC ACGTCTACCA 
GAGAGAGCCA GCCAGACGAG TAGCAGTTCA ACGATTTCCA CCTCTTCCAC GCCCACAGTC 
TTGGCCGCCT CCCCACCTTC TCAGCACACT GTCCTGAAAG GATCCCCCAG ACCTTCTGTT 
TTGTCATTGC CAGAGTCGAA TGATGCTCTA AAGAAAGAGT CCAGTAAACC CAGTGGTAAA 
GTGTTGGCAT ACTCTGATGT GGATGACATT TTTGCCTCCG ACGTTCTGTT TGAGGCCACG 
TCGGTGACAA ACACACCCCC CACCGTACTG TCAACCGCGA AGACACCACC TGTAGGCAGC 
GGCGTGTTGG CTGCTAAAAA AGACAAAGAC AAAAGCACAT TCCAGTCTAT TTTTGATGAC 
GATGTGGATG ATTTGTTTCA ACCAGCCAAA CCGAGGTCGA CGGCCAAGAA AGCCAAGCAG 
TCGAAATTCT TGGAAGACGA CGATGATGAC GAGGACATAT TCGGTGTCAG CAACAGTTCC 
ACTCCGTCAT CGACCAGTAG TAAAGAAATG AAGAATGGTA GTAGTTTTTC CAAGCAGGAA 
ATTTTCCAGG ACGAAGTAAC TACTGTGCCT AAAGTTCAAA AGAAGCACAA AGAGAAGCCT 
TTAGATTTCA GCTTGTTCGA TGATGACACA GACATTTTTG CTGATCTGAC AGACACTTTC 
AAACCAAAGC AGAAACTCAA GACAAAGCCA GAGGCCAAGT CGATATTTGA CGATGACATG 
GATGACATCT TCTCATCGAG CAAAGTGAAG AAAGTTTCAA AGGCTTCAAA TAAATCTAAG 
AAAACGATAC CCGCCCAGGA CACGACTGCA GCGACAGATT CCAGCAACAT ATTTGACGAT 
CCACTAAACG CCCTTGGTGG GACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103387313 ( XM_008321891.2 ) cDNA ORF clone, Cynoglossus semilaevis(tongue sole) -> XP_008320113.1 Cynoglossus semilaevis WASH complex subunit FAM21-like (LOC103387313), transcript variant X1, mRNA.

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CloneID	OCy39598
Clone ID Related Accession (Same CDS sequence)	XM_008321891.3 , XM_008321891.2
Accession Version	XM_008321891.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-06
Organism	Cynoglossus semilaevis(tongue sole)
Product	WASH complex subunit FAM21-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024318.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 7, 2016 this sequence version replaced XM_008321891.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cynoglossus semilaevis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGTT TTCCGGGGGG AATAGCAAAT GGTCCCAGCC AGAGTGAACA CATGGAGAAA 
GATGCCCGGG TATGGGAGAG ACCCTGGACG TTGGAAGAAA TGCGGCAGAG TAGTGCCAGC 
TGGTCCTTGG CAGCAGATTC AGGGCTCTTC CTGTTTCTGC AAGACTTCTC TCACAAAATG 
CTGTCAAAGA CGCATGAGAT TGAAAAACAA TTGGATGGCC TCATCCGTGA CACCAAGGCT 
ACAGACAGCT GCCTGCACTC TGTGTTCAGT GACTTCCTCA TGCTCTCCAA TACACAGTTT 
ATAGAAAATA GAGTATGTGA TGAAGAAGTC GAAGAACCAC TGCCTAAAAC TGATGCTGTG 
GAGAAACAAC CGGAACGGAC CCGGGAGCAA AAGGAGGCGG AGCTGATCCC TAAGATGCAG 
GAAGCTGTAA ATTACGGTCT ACGAGTGCTG GAGTCGGCGT TTGAGCATCT GGACATCAAG 
GCAGGGAACT CGGATTCAGA GGATGAGGAG GTCACGGGCA GAGTAGAGGC CATCCTGGAA 
CCTAAGGATC TTTATGTGGA CAGACCACTT CCCTATCTGA TTGGTTCCCA GGCGTTCATG 
GAACAGGATG ATGTTGGACT TGGCGACCTC TCCAGTGATG AAATGTCAGT AGACAGTGAC 
AGAGACAGCG TGATTGAAAG TGACGACGGC AAAGAAGCCA CACATTCGGA TGAAGATTTT 
ATTCAAGAAG GCCAGGTTCA CAACATCTTC AAAAAGAAAT CATCTATGTT GAGCTTCGAA 
GATGACGACG AGGAAGAGGA AGACGAGGAT TCGGACATAT TTGGAGAATC AGACAAGGAT 
GATGACGACC ACGACCCGAA GAACTCGGGT CCAATATCAT TTGCTGATGA GCTGGCAGCT 
CGGATCAGAG GTGAACCAGT TCATAAACAG GAGGGAGATC GTGCCTCATT GTCATCTAAG 
AAGAAAAGTA AAGGCAGAAA AGAAGCAAAG CCCTCAAAGA CCCAGGCTGA AGATGACAGT 
GACGACATGT TCAAACCACC CAAGATGGAT GAGATCGACG ATGACGATGA TGACGATGAC 
TTTTCCCCGT TTGGAGGAAG TAGCCGCCTC TTTAGTGGGG GGAAGGGCCT GTTTGATGAT 
GACAACGACG AGCGGGACCT TTTCGATGAT GCACCAAAAC CTTTTGTACC AGAAGAAAAG 
AAACCTGTAA AAGAAAGTGT GAAAGTCACA GCCACAACAG CAGAACCTAG GAAATCTGCA 
AAGAAGATTC CAACAGGTGG CGTTTCAATA TTTCCAGCCA ACAGCCTCTT CAGTCCAGGG 
AATGATTCTG ATTCTGTTAG AAGCAAGGAG AACGGAACTC CGAATTCCAA GGTCAGCGCT 
CCTCCAAAAG TTGAAAAAAA GACTGCTGTT GGTGGTGGAG GACTCTTTGA TGAGGAGGAT 
GAGGACGACG ACTTCTTTGG TGGAAAAAGT CTACAAAAGT CTGAATCTGT TGTAAAGGAG 
AAACCTAAAT CCAAGACAAC CGTTGACCTC TTTGATGAAG ATGATGAGGA TGATGGCAAT 
ATATTCGGTG ATAAATTTAA GTCAACAACT CCAGTTCAGG TCAAGAAGGA GGTTGTTGCA 
GAACCAGTTA AACCGCCTGA GAAGAAGATG CCAGCTGGGG CCATCTCCAT GTTTGGACCT 
GGAACTAAAA GTCTCCTCAG CGAAGGCCTG AAAAAGCGTC GACAATCGAC CAGTGAGGAG 
TCGGAAAAAT CTGAGGAGAA CGGTGCTTCT CCAGATGTTA GGAAAGCTGC TCCAAAACCA 
TCCCCGAAAC CCCAGACCAG GGGTCTTTTC TCTGATGATG AAGATGCACA AGCTTTTCCA 
ACCATCCCAA GAAGTCAATC GAAGCCTGAA CCTACAAACC AGAGCAACAC CAACAAAGCT 
CCTGTGTTCT TGTTTGATGA CGATGAAGAA GAGGATCTCT TTGCTTCTGC GGTTAAATCT 
AAACCAAAAG CTAGTTCATC TAAAACCTCA CCCATGCAAA CCAGCAGTGC AGTCTCTAGC 
TCCCTCTTCA GTGATGATGA GGATCAGTGG TTAGGTGCTA CAAAAACATC AGAGACCAAC 
TCAGGAGGAA TGAAGGCCAG TGCTAGCGCC CCCTCTGGTC TCACTAGTAC CAAAACAACA 
CCCAGCAGCA GCCTGTTTGA GGAGGAAGAT GATAATGAAC TGTTTGCACA AACAAAAGAA 
TCAAGTACTC AGAAAAACCC TCAGCCAGTT GCACTACTGT TTGAAGATAA TGGTGGTGAT 
GATGAAGATG ACGAAGATCA AGGAACCCTT TTTAATGTCA ACACAAATAC TGCACTACCA 
ACTACTAAAA CACTAGCCTC AGCATCCAAG TCTTCCTCAT CCCAGATGCC TAAATCAGAC 
ACAGTAGAAC ATGTACCCAC AGAAAACCAG TCTTTGCAGC AGGAGAAGCT GACTCCACAT 
ACTTCAAAGT CTCCAGAGAT GCCTGTGGGA GAAAGTGGAG AAAGTAAAAA GAAGCCTGCT 
AGGGCTGTTA GTCTCTTCGG AGGCATTAAT GTTCTTGAAA AGCAGACAAA AACCCTAATA 
CAGGATGAAG AAGAAGAAGA AGATGATGAC TTCCCTTTGA AGCCCAGCCC TCCCCCATTT 
GTCATGGAGG AGAAGGAAGA AAAATCTAGA TCAGGGGCTT TTGGTCTGTT TGAAGATGAA 
GACGACGACG AGTGGAATGA TTCTATTTTC ACACCTGGTA AACCCACCAG CAAAACAACA 
CCCAAGGAAG AAGAAGAGGA GGAGGAGGAG GAGGCGGCGG AGGCGGAGGA GAAAGAGGAG 
GTGGAGCCAG CACAGACAAA GAGCACGGGT GTTCTCCAGG ACGAGGAGTT GTTCAGCCAC 
AGTCATGAGA AAGACAATGA CCTCGACGCC GGTCTCTTTG CCACTTCAGG GAGAGCTGAA 
AGCCCACCAA AGGCAGCAGC ACATAATTTG TTTGCAGAAG ACGATGAAGA TGACCTTTTC 
AGCTCCCCCA AGCCAAAAGT TCTTCCAAAA ATTGCAGAAA AGCCAAGTAA ACCTGCTGAC 
AAAGCACCGC TGGCCAGTCC AGAACCTGTC TCAGAGACTG AGAAACCTGC ACCAAGTCCT 
GTAAAACCTA AAGCTTCCTC ATCAAGGATT GGAAAACTTC AAGCAAGTCT CAAGATCAAT 
CCTGCTGCTA TGCTTCCTGG TGCTGTTCCC ACTTTGCCAG GTGCCATCAG TGTCTTCCCT 
GGCATGGATC CCAGCTCCTC CTCCGGTGTG TCAAGCTCCA GCCTGAGCCC CAGCCCTGTG 
ACAACTCCTG TAGGGGCTCA GACAGACAGT GAGGGAGTGA CCTTTGACAC CCCAGTCCAG 
GTCACGACAC TGCAGAGTGT ACAGAAAAGT CGCACCAAAG GTGCTGGTCA GCGCAGACCC 
CAGACCAGAG CTGCAAGACA GAAAGCTGTA CAAAGATCAG TCGACGACAG AGACAATTTC 
ACAAGTACAG CTGCGTCAGG TCCAAACCCC AATCTGTCTG ATTCAGAGTC ACGTCTACCA 
GAGAGAGCCA GCCAGACGAG TAGCAGTTCA ACGATTTCCA CCTCTTCCAC GCCCACAGTC 
TTGGCCGCCT CCCCACCTTC TCAGCACACT GTCCTGAAAG GATCCCCCAG ACCTTCTGTT 
TTGTCATTGC CAGAGTCGAA TGATGCTCTA AAGAAAGAGT CCAGTAAACC CAGTGGTAAA 
GTGTTGGCAT ACTCTGATGT GGATGACATT TTTGCCTCCG ACGTTCTGTT TGAGGCCACG 
TCGGTGACAA ACACACCCCC CACCGTACTG TCAACCGCGA AGACACCACC TGTAGGCAGC 
GGCGTGTTGG CTGCTAAAAA AGACAAAGAC AAAAGCACAT TCCAGTCTAT TTTTGATGAC 
GATGTGGATG ATTTGTTTCA ACCAGCCAAA CCGAGGTCGA CGGCCAAGAA AGCCAAGCAG 
TCGAAATTCT TGGAAGACGA CGATGATGAC GAGGACATAT TCGGTGTCAG CAACAGTTCC 
ACTCCGTCAT CGACCAGTAG TAAAGAAATG AAGAATGGTA GTAGTTTTTC CAAGCAGGAA 
ATTTTCCAGG ACGAAGTAAC TACTGTGCCT AAAGTTCAAA AGAAGCACAA AGAGAAGCCT 
TTAGATTTCA GCTTGTTCGA TGATGACACA GACATTTTTG CTGATCTGAC AGACACTTTC 
AAACCAAAGC AGAAACTCAA GACAAAGCCA GAGGCCAAGT CGATATTTGA CGATGACATG 
GATGACATCT TCTCATCGAG CAAAGTGAAG AAAGTTTCAA AGGCTTCAAA TAAATCTAAG 
AAAACGATAC CCGCCCAGGA CACGACTGCA GCGACAGATT CCAGCAACAT ATTTGACGAT 
CCACTAAACG CCCTTGGTGG GACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008320113.1
CDS	141..4547
Translation	MSGFPGGIANGPSQSEHMEKDARVWERPWTLEEMRQSSASWSLAADSGLFLFLQDFSHKMLSKTHEIEKQLDGLIRDTKATDSCLHSVFSDFLMLSNTQFIENRVCDEEVEEPLPKTDAVEKQPERTREQKEAELIPKMQEAVNYGLRVLESAFEHLDIKAGNSDSEDEEVTGRVEAILEPKDLYVDRPLPYLIGSQAFMEQDDVGLGDLSSDEMSVDSDRDSVIESDDGKEATHSDEDFIQEGQVHNIFKKKSSMLSFEDDDEEEEDEDSDIFGESDKDDDDHDPKNSGPISFADELAARIRGEPVHKQEGDRASLSSKKKSKGRKEAKPSKTQAEDDSDDMFKPPKMDEIDDDDDDDDFSPFGGSSRLFSGGKGLFDDDNDERDLFDDAPKPFVPEEKKPVKESVKVTATTAEPRKSAKKIPTGGVSIFPANSLFSPGNDSDSVRSKENGTPNSKVSAPPKVEKKTAVGGGGLFDEEDEDDDFFGGKSLQKSESVVKEKPKSKTTVDLFDEDDEDDGNIFGDKFKSTTPVQVKKEVVAEPVKPPEKKMPAGAISMFGPGTKSLLSEGLKKRRQSTSEESEKSEENGASPDVRKAAPKPSPKPQTRGLFSDDEDAQAFPTIPRSQSKPEPTNQSNTNKAPVFLFDDDEEEDLFASAVKSKPKASSSKTSPMQTSSAVSSSLFSDDEDQWLGATKTSETNSGGMKASASAPSGLTSTKTTPSSSLFEEEDDNELFAQTKESSTQKNPQPVALLFEDNGGDDEDDEDQGTLFNVNTNTALPTTKTLASASKSSSSQMPKSDTVEHVPTENQSLQQEKLTPHTSKSPEMPVGESGESKKKPARAVSLFGGINVLEKQTKTLIQDEEEEEDDDFPLKPSPPPFVMEEKEEKSRSGAFGLFEDEDDDEWNDSIFTPGKPTSKTTPKEEEEEEEEEAAEAEEKEEVEPAQTKSTGVLQDEELFSHSHEKDNDLDAGLFATSGRAESPPKAAAHNLFAEDDEDDLFSSPKPKVLPKIAEKPSKPADKAPLASPEPVSETEKPAPSPVKPKASSSRIGKLQASLKINPAAMLPGAVPTLPGAISVFPGMDPSSSSGVSSSSLSPSPVTTPVGAQTDSEGVTFDTPVQVTTLQSVQKSRTKGAGQRRPQTRAARQKAVQRSVDDRDNFTSTAASGPNPNLSDSESRLPERASQTSSSSTISTSSTPTVLAASPPSQHTVLKGSPRPSVLSLPESNDALKKESSKPSGKVLAYSDVDDIFASDVLFEATSVTNTPPTVLSTAKTPPVGSGVLAAKKDKDKSTFQSIFDDDVDDLFQPAKPRSTAKKAKQSKFLEDDDDDEDIFGVSNSSTPSSTSSKEMKNGSSFSKQEIFQDEVTTVPKVQKKHKEKPLDFSLFDDDTDIFADLTDTFKPKQKLKTKPEAKSIFDDDMDDIFSSSKVKKVSKASNKSKKTIPAQDTTAATDSSNIFDDPLNALGGT

Target ORF information:

RefSeq Version	XM_008321891.2
Organism	Cynoglossus semilaevis(tongue sole)
Definition	Cynoglossus semilaevis WASH complex subunit FAM21-like (LOC103387313), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008321891.2

ORF Insert Sequence:

ATGAGCGGTT TTCCGGGGGG AATAGCAAAT GGTCCCAGCC AGAGTGAACA CATGGAGAAA 
GATGCCCGGG TATGGGAGAG ACCCTGGACG TTGGAAGAAA TGCGGCAGAG TAGTGCCAGC 
TGGTCCTTGG CAGCAGATTC AGGGCTCTTC CTGTTTCTGC AAGACTTCTC TCACAAAATG 
CTGTCAAAGA CGCATGAGAT TGAAAAACAA TTGGATGGCC TCATCCGTGA CACCAAGGCT 
ACAGACAGCT GCCTGCACTC TGTGTTCAGT GACTTCCTCA TGCTCTCCAA TACACAGTTT 
ATAGAAAATA GAGTATGTGA TGAAGAAGTC GAAGAACCAC TGCCTAAAAC TGATGCTGTG 
GAGAAACAAC CGGAACGGAC CCGGGAGCAA AAGGAGGCGG AGCTGATCCC TAAGATGCAG 
GAAGCTGTAA ATTACGGTCT ACGAGTGCTG GAGTCGGCGT TTGAGCATCT GGACATCAAG 
GCAGGGAACT CGGATTCAGA GGATGAGGAG GTCACGGGCA GAGTAGAGGC CATCCTGGAA 
CCTAAGGATC TTTATGTGGA CAGACCACTT CCCTATCTGA TTGGTTCCCA GGCGTTCATG 
GAACAGGATG ATGTTGGACT TGGCGACCTC TCCAGTGATG AAATGTCAGT AGACAGTGAC 
AGAGACAGCG TGATTGAAAG TGACGACGGC AAAGAAGCCA CACATTCGGA TGAAGATTTT 
ATTCAAGAAG GCCAGGTTCA CAACATCTTC AAAAAGAAAT CATCTATGTT GAGCTTCGAA 
GATGACGACG AGGAAGAGGA AGACGAGGAT TCGGACATAT TTGGAGAATC AGACAAGGAT 
GATGACGACC ACGACCCGAA GAACTCGGGT CCAATATCAT TTGCTGATGA GCTGGCAGCT 
CGGATCAGAG GTGAACCAGT TCATAAACAG GAGGGAGATC GTGCCTCATT GTCATCTAAG 
AAGAAAAGTA AAGGCAGAAA AGAAGCAAAG CCCTCAAAGA CCCAGGCTGA AGATGACAGT 
GACGACATGT TCAAACCACC CAAGATGGAT GAGATCGACG ATGACGATGA TGACGATGAC 
TTTTCCCCGT TTGGAGGAAG TAGCCGCCTC TTTAGTGGGG GGAAGGGCCT GTTTGATGAT 
GACAACGACG AGCGGGACCT TTTCGATGAT GCACCAAAAC CTTTTGTACC AGAAGAAAAG 
AAACCTGTAA AAGAAAGTGT GAAAGTCACA GCCACAACAG CAGAACCTAG GAAATCTGCA 
AAGAAGATTC CAACAGGTGG CGTTTCAATA TTTCCAGCCA ACAGCCTCTT CAGTCCAGGG 
AATGATTCTG ATTCTGTTAG AAGCAAGGAG AACGGAACTC CGAATTCCAA GGTCAGCGCT 
CCTCCAAAAG TTGAAAAAAA GACTGCTGTT GGTGGTGGAG GACTCTTTGA TGAGGAGGAT 
GAGGACGACG ACTTCTTTGG TGGAAAAAGT CTACAAAAGT CTGAATCTGT TGTAAAGGAG 
AAACCTAAAT CCAAGACAAC CGTTGACCTC TTTGATGAAG ATGATGAGGA TGATGGCAAT 
ATATTCGGTG ATAAATTTAA GTCAACAACT CCAGTTCAGG TCAAGAAGGA GGTTGTTGCA 
GAACCAGTTA AACCGCCTGA GAAGAAGATG CCAGCTGGGG CCATCTCCAT GTTTGGACCT 
GGAACTAAAA GTCTCCTCAG CGAAGGCCTG AAAAAGCGTC GACAATCGAC CAGTGAGGAG 
TCGGAAAAAT CTGAGGAGAA CGGTGCTTCT CCAGATGTTA GGAAAGCTGC TCCAAAACCA 
TCCCCGAAAC CCCAGACCAG GGGTCTTTTC TCTGATGATG AAGATGCACA AGCTTTTCCA 
ACCATCCCAA GAAGTCAATC GAAGCCTGAA CCTACAAACC AGAGCAACAC CAACAAAGCT 
CCTGTGTTCT TGTTTGATGA CGATGAAGAA GAGGATCTCT TTGCTTCTGC GGTTAAATCT 
AAACCAAAAG CTAGTTCATC TAAAACCTCA CCCATGCAAA CCAGCAGTGC AGTCTCTAGC 
TCCCTCTTCA GTGATGATGA GGATCAGTGG TTAGGTGCTA CAAAAACATC AGAGACCAAC 
TCAGGAGGAA TGAAGGCCAG TGCTAGCGCC CCCTCTGGTC TCACTAGTAC CAAAACAACA 
CCCAGCAGCA GCCTGTTTGA GGAGGAAGAT GATAATGAAC TGTTTGCACA AACAAAAGAA 
TCAAGTACTC AGAAAAACCC TCAGCCAGTT GCACTACTGT TTGAAGATAA TGGTGGTGAT 
GATGAAGATG ACGAAGATCA AGGAACCCTT TTTAATGTCA ACACAAATAC TGCACTACCA 
ACTACTAAAA CACTAGCCTC AGCATCCAAG TCTTCCTCAT CCCAGATGCC TAAATCAGAC 
ACAGTAGAAC ATGTACCCAC AGAAAACCAG TCTTTGCAGC AGGAGAAGCT GACTCCACAT 
ACTTCAAAGT CTCCAGAGAT GCCTGTGGGA GAAAGTGGAG AAAGTAAAAA GAAGCCTGCT 
AGGGCTGTTA GTCTCTTCGG AGGCATTAAT GTTCTTGAAA AGCAGACAAA AACCCTAATA 
CAGGATGAAG AAGAAGAAGA AGATGATGAC TTCCCTTTGA AGCCCAGCCC TCCCCCATTT 
GTCATGGAGG AGAAGGAAGA AAAATCTAGA TCAGGGGCTT TTGGTCTGTT TGAAGATGAA 
GACGACGACG AGTGGAATGA TTCTATTTTC ACACCTGGTA AACCCACCAG CAAAACAACA 
CCCAAGGAAG AAGAAGAGGA GGAGGAGGAG GAGGCGGCGG AGGCGGAGGA GAAAGAGGAG 
GTGGAGCCAG CACAGACAAA GAGCACGGGT GTTCTCCAGG ACGAGGAGTT GTTCAGCCAC 
AGTCATGAGA AAGACAATGA CCTCGACGCC GGTCTCTTTG CCACTTCAGG GAGAGCTGAA 
AGCCCACCAA AGGCAGCAGC ACATAATTTG TTTGCAGAAG ACGATGAAGA TGACCTTTTC 
AGCTCCCCCA AGCCAAAAGT TCTTCCAAAA ATTGCAGAAA AGCCAAGTAA ACCTGCTGAC 
AAAGCACCGC TGGCCAGTCC AGAACCTGTC TCAGAGACTG AGAAACCTGC ACCAAGTCCT 
GTAAAACCTA AAGCTTCCTC ATCAAGGATT GGAAAACTTC AAGCAAGTCT CAAGATCAAT 
CCTGCTGCTA TGCTTCCTGG TGCTGTTCCC ACTTTGCCAG GTGCCATCAG TGTCTTCCCT 
GGCATGGATC CCAGCTCCTC CTCCGGTGTG TCAAGCTCCA GCCTGAGCCC CAGCCCTGTG 
ACAACTCCTG TAGGGGCTCA GACAGACAGT GAGGGAGTGA CCTTTGACAC CCCAGTCCAG 
GTCACGACAC TGCAGAGTGT ACAGAAAAGT CGCACCAAAG GTGCTGGTCA GCGCAGACCC 
CAGACCAGAG CTGCAAGACA GAAAGCTGTA CAAAGATCAG TCGACGACAG AGACAATTTC 
ACAAGTACAG CTGCGTCAGG TCCAAACCCC AATCTGTCTG ATTCAGAGTC ACGTCTACCA 
GAGAGAGCCA GCCAGACGAG TAGCAGTTCA ACGATTTCCA CCTCTTCCAC GCCCACAGTC 
TTGGCCGCCT CCCCACCTTC TCAGCACACT GTCCTGAAAG GATCCCCCAG ACCTTCTGTT 
TTGTCATTGC CAGAGTCGAA TGATGCTCTA AAGAAAGAGT CCAGTAAACC CAGTGGTAAA 
GTGTTGGCAT ACTCTGATGT GGATGACATT TTTGCCTCCG ACGTTCTGTT TGAGGCCACG 
TCGGTGACAA ACACACCCCC CACCGTACTG TCAACCGCGA AGACACCACC TGTAGGCAGC 
GGCGTGTTGG CTGCTAAAAA AGACAAAGAC AAAAGCACAT TCCAGTCTAT TTTTGATGAC 
GATGTGGATG ATTTGTTTCA ACCAGCCAAA CCGAGGTCGA CGGCCAAGAA AGCCAAGCAG 
TCGAAATTCT TGGAAGACGA CGATGATGAC GAGGACATAT TCGGTGTCAG CAACAGTTCC 
ACTCCGTCAT CGACCAGTAG TAAAGAAATG AAGAATGGTA GTAGTTTTTC CAAGCAGGAA 
ATTTTCCAGG ACGAAGTAAC TACTGTGCCT AAAGTTCAAA AGAAGCACAA AGAGAAGCCT 
TTAGATTTCA GCTTGTTCGA TGATGACACA GACATTTTTG CTGATCTGAC AGACACTTTC 
AAACCAAAGC AGAAACTCAA GACAAAGCCA GAGGCCAAGT CGATATTTGA CGATGACATG 
GATGACATCT TCTCATCGAG CAAAGTGAAG AAAGTTTCAA AGGCTTCAAA TAAATCTAAG 
AAAACGATAC CCGCCCAGGA CACGACTGCA GCGACAGATT CCAGCAACAT ATTTGACGAT 
CCACTAAACG CCCTTGGTGG GACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103387313 ( XM_008321893.3 ) cDNA ORF clone, Cynoglossus semilaevis(tongue sole) -> XP_008320115.1 Cynoglossus semilaevis WASH complex subunit 2-like (LOC103387313), transcript variant X2, mRNA.

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CloneID	OCy39599
Clone ID Related Accession (Same CDS sequence)	XM_008321893.3 , XM_008321893.2
Accession Version	XM_008321893.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4347bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-15
Organism	Cynoglossus semilaevis(tongue sole)
Product	WASH complex subunit 2-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024318.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 16, 2018 this sequence version replaced XM_008321893.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cynoglossus semilaevis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGTT TTCCGGGGGG AATAGCAAAT GGTCCCAGCC AGAGTGAACA CATGGAGAAA 
GATGCCCGGG TATGGGAGAG ACCCTGGACG TTGGAAGAAA TGCGGCAGAG TAGTGCCAGC 
TGGTCCTTGG CAGCAGATTC AGGGCTCTTC CTGTTTCTGC AAGACTTCTC TCACAAAATG 
CTGTCAAAGA CGCATGAGAT TGAAAAACAA TTGGATGGCC TCATCCGTGA CACCAAGGCT 
ACAGACAGCT GCCTGCACTC TGTGTTCAGT GACTTCCTCA TGCTCTCCAA TACACAGTTT 
ATAGAAAATA GAGTATGTGA TGAAGAAGTC GAAGAACCAC TGCCTAAAAC TGATGCTGTG 
GAGAAACAAC CGGAACGGAC CCGGGAGCAA AAGGAGGCGG AGCTGATCCC TAAGATGCAG 
GAAGCTGTAA ATTACGGTCT ACGAGTGCTG GAGTCGGCGT TTGAGCATCT GGACATCAAG 
GCAGGGAACT CGGATTCAGA GGATGAGGAG GTCACGGGCA GAGTAGAGGC CATCCTGGAA 
CCTAAGGATC TTTATGTGGA CAGACCACTT CCCTATCTGA TTGGTTCCCA GGCGTTCATG 
GAACAGGATG ATGTTGGACT TGGCGACCTC TCCAGTGATG AAATGTCAGT AGACAGTGAC 
AGAGACAGCG TGATTGAAAG TGACGACGGC AAAGAAGCCA CACATTCGGA TGAAGATTTT 
ATTCAAGAAG GCCAGGTTCA CAACATCTTC AAAAAGAAAT CATCTATGTT GAGCTTCGAA 
GATGACGACG AGGAAGAGGA AGACGAGGAT TCGGACATAT TTGGAGAATC AGACAAGGAT 
GATGACGACC ACGACCCGAA GAACTCGGGT CCAATATCAT TTGCTGATGA GCTGGCAGCT 
CGGATCAGAG GTGAACCAGT TCATAAACAG GAGGGAGATC GTGCCTCATT GTCATCTAAG 
AAGAAAAGTA AAGGCAGAAA AGAAGCAAAG CCCTCAAAGA CCCAGGCTGA AGATGACAGT 
GACGACATGT TCAAACCACC CAAGATGGAT GAGATCGACG ATGACGATGA TGACGATGAC 
TTTTCCCCGT TTGGAGGAAG TAGCCGCCTC TTTAGTGGGG GGAAGGGCCT GTTTGATGAT 
GACAACGACG AGCGGGACCT TTTCGATGAT GCACCAAAAC CTTTTGTACC AGAAGAAAAG 
AAACCTGTAA AAGAAAGTGT GAAAGTCACA GCCACAACAG CAGAACCTAG GAAATCTGCA 
AAGAAGATTC CAACAGGTGG CGTTTCAATA TTTCCAGCCA ACAGCCTCTT CAGTCCAGGG 
AATGATTCTG ATTCTGTTAG AAGCAAGGAG AACGGAACTC CGAATTCCAA GGTCAGCGCT 
CCTCCAAAAG TTGAAAAAAA GACTGCTGTT GGTGGTGGAG GACTCTTTGA TGAGGAGGAT 
GAGGACGACG ACTTCTTTGG TGGAAAAAGT CTACAAAAGT CTGAATCTGT TGTAAAGGAG 
AAACCTAAAT CCAAGACAAC CGTTGACCTC TTTGATGAAG ATGATGAGGA TGATGGCAAT 
ATATTCGGTG ATAAATTTAA GTCAACAACT CCAGTTCAGG TCAAGAAGGA GGTTGTTGCA 
GAACCAGTTA AACCGCCTGA GAAGAAGATG CCAGCTGGGG CCATCTCCAT GTTTGGACCT 
GGAACTAAAA GTCTCCTCAG CGAAGGCCTG AAAAAGCGTC GACAATCGAC CAGTGAGGAG 
TCGGAAAAAT CTGAGGAGAA CGGTGCTTCT CCAGATGTTA GGAAAGCTGC TCCAAAACCA 
TCCCCGAAAC CCCAGACCAG GGGTCTTTTC TCTGATGATG AAGATGCACA AGCTTTTCCA 
ACCATCCCAA GAAGTCAATC GAAGCCTGAA CCTACAAACC AGAGCAACAC CAACAAAGCT 
CCTGTGTTCT TGTTTGATGA CGATGAAGAA GAGGATCTCT TTGCTTCTGC GGTTAAATCT 
AAACCAAAAG CTAGTTCATC TAAAACCTCA CCCATGCAAA CCAGCAGTGC AGTCTCTAGC 
TCCCTCTTCA GTGATGATGA GGATCAGTGG TTAGGTGCTA CAAAAACATC AGAGACCAAC 
TCAGGAGGAA TGAAGGCCAG TGCTAGCGCC CCCTCTGGTC TCACTAGTAC CAAAACAACA 
CCCAGCAGCA GCCTGTTTGA GGAGGAAGAT GATAATGAAC TGTTTGCACA AACAAAAGAA 
TCAAGTACTC AGAAAAACCC TCAGCCAGTT GCACTACTGT TTGAAGATAA TGGTGGTGAT 
GATGAAGATG ACGAAGATCA AGGAACCCTT TTTAATGTCA ACACAAATAC TGCACTACCA 
ACTACTAAAA CACTAGCCTC AGCATCCAAG TCTTCCTCAT CCCAGATGCC TAAATCAGAC 
ACAGTAGAAC ATGTACCCAC AGAAAACCAG TCTTTGCAGC AGGAGAAGCT GACTCCACAT 
ACTTCAAAGT CTCCAGAGAT GCCTGTGGGA GAAAGTGGAG AAAGTAAAAA GAAGCCTGCT 
AGGGCTGTTA GTCTCTTCGG AGGCATTAAT GTTCTTGAAA AGCAGACAAA AACCCTAATA 
CAGGATGAAG AAGAAGAAGA AGATGATGAC TTCCCTTTGA AGCCCAGCCC TCCCCCATTT 
GTCATGGAGG AGAAGGAAGA AAAATCTAGA TCAGGGGCTT TTGGTCTGTT TGAAGATGAA 
GACGACGACG AGTGGAATGA TTCTATTTTC ACACCTGGTA AACCCACCAG CAAAACAACA 
CCCAAGGAAG AAGAAGAGGA GGAGGAGGAG GAGGCGGCGG AGGCGGAGGA GAAAGAGGAG 
GTGGAGCCAG CACAGACAAA GAGCACGGGT GTTCTCCAGG ACGAGGAGTT GTTCAGCCAC 
AGTCATGAGA AAGACAATGA CCTCGACGCC GGTCTCTTTG CCACTTCAGG GAGAGCTGAA 
AGCCCACCAA AGGCAGCAGC ACATAATTTG TTTGCAGAAG ACGATGAAGA TGACCTTTTC 
AGCTCCCCCA AGCCAAAAGT TCTTCCAAAA ATTGCAGAAA AGCCAAGTAA ACCTGCTGAC 
AAAGCACCGC TGGCCAGTCC AGAACCTGTC TCAGAGACTG AGGCAAGTCT CAAGATCAAT 
CCTGCTGCTA TGCTTCCTGG TGCTGTTCCC ACTTTGCCAG GTGCCATCAG TGTCTTCCCT 
GGCATGGATC CCAGCTCCTC CTCCGGTGTG TCAAGCTCCA GCCTGAGCCC CAGCCCTGTG 
ACAACTCCTG TAGGGGCTCA GACAGACAGT GAGGGAGTGA CCTTTGACAC CCCAGTCCAG 
GTCACGACAC TGCAGAGTGT ACAGAAAAGT CGCACCAAAG GTGCTGGTCA GCGCAGACCC 
CAGACCAGAG CTGCAAGACA GAAAGCTGTA CAAAGATCAG TCGACGACAG AGACAATTTC 
ACAAGTACAG CTGCGTCAGG TCCAAACCCC AATCTGTCTG ATTCAGAGTC ACGTCTACCA 
GAGAGAGCCA GCCAGACGAG TAGCAGTTCA ACGATTTCCA CCTCTTCCAC GCCCACAGTC 
TTGGCCGCCT CCCCACCTTC TCAGCACACT GTCCTGAAAG GATCCCCCAG ACCTTCTGTT 
TTGTCATTGC CAGAGTCGAA TGATGCTCTA AAGAAAGAGT CCAGTAAACC CAGTGGTAAA 
GTGTTGGCAT ACTCTGATGT GGATGACATT TTTGCCTCCG ACGTTCTGTT TGAGGCCACG 
TCGGTGACAA ACACACCCCC CACCGTACTG TCAACCGCGA AGACACCACC TGTAGGCAGC 
GGCGTGTTGG CTGCTAAAAA AGACAAAGAC AAAAGCACAT TCCAGTCTAT TTTTGATGAC 
GATGTGGATG ATTTGTTTCA ACCAGCCAAA CCGAGGTCGA CGGCCAAGAA AGCCAAGCAG 
TCGAAATTCT TGGAAGACGA CGATGATGAC GAGGACATAT TCGGTGTCAG CAACAGTTCC 
ACTCCGTCAT CGACCAGTAG TAAAGAAATG AAGAATGGTA GTAGTTTTTC CAAGCAGGAA 
ATTTTCCAGG ACGAAGTAAC TACTGTGCCT AAAGTTCAAA AGAAGCACAA AGAGAAGCCT 
TTAGATTTCA GCTTGTTCGA TGATGACACA GACATTTTTG CTGATCTGAC AGACACTTTC 
AAACCAAAGC AGAAACTCAA GACAAAGCCA GAGGCCAAGT CGATATTTGA CGATGACATG 
GATGACATCT TCTCATCGAG CAAAGTGAAG AAAGTTTCAA AGGCTTCAAA TAAATCTAAG 
AAAACGATAC CCGCCCAGGA CACGACTGCA GCGACAGATT CCAGCAACAT ATTTGACGAT 
CCACTAAACG CCCTTGGTGG GACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008320115.1
CDS	156..4502
Translation	MSGFPGGIANGPSQSEHMEKDARVWERPWTLEEMRQSSASWSLAADSGLFLFLQDFSHKMLSKTHEIEKQLDGLIRDTKATDSCLHSVFSDFLMLSNTQFIENRVCDEEVEEPLPKTDAVEKQPERTREQKEAELIPKMQEAVNYGLRVLESAFEHLDIKAGNSDSEDEEVTGRVEAILEPKDLYVDRPLPYLIGSQAFMEQDDVGLGDLSSDEMSVDSDRDSVIESDDGKEATHSDEDFIQEGQVHNIFKKKSSMLSFEDDDEEEEDEDSDIFGESDKDDDDHDPKNSGPISFADELAARIRGEPVHKQEGDRASLSSKKKSKGRKEAKPSKTQAEDDSDDMFKPPKMDEIDDDDDDDDFSPFGGSSRLFSGGKGLFDDDNDERDLFDDAPKPFVPEEKKPVKESVKVTATTAEPRKSAKKIPTGGVSIFPANSLFSPGNDSDSVRSKENGTPNSKVSAPPKVEKKTAVGGGGLFDEEDEDDDFFGGKSLQKSESVVKEKPKSKTTVDLFDEDDEDDGNIFGDKFKSTTPVQVKKEVVAEPVKPPEKKMPAGAISMFGPGTKSLLSEGLKKRRQSTSEESEKSEENGASPDVRKAAPKPSPKPQTRGLFSDDEDAQAFPTIPRSQSKPEPTNQSNTNKAPVFLFDDDEEEDLFASAVKSKPKASSSKTSPMQTSSAVSSSLFSDDEDQWLGATKTSETNSGGMKASASAPSGLTSTKTTPSSSLFEEEDDNELFAQTKESSTQKNPQPVALLFEDNGGDDEDDEDQGTLFNVNTNTALPTTKTLASASKSSSSQMPKSDTVEHVPTENQSLQQEKLTPHTSKSPEMPVGESGESKKKPARAVSLFGGINVLEKQTKTLIQDEEEEEDDDFPLKPSPPPFVMEEKEEKSRSGAFGLFEDEDDDEWNDSIFTPGKPTSKTTPKEEEEEEEEEAAEAEEKEEVEPAQTKSTGVLQDEELFSHSHEKDNDLDAGLFATSGRAESPPKAAAHNLFAEDDEDDLFSSPKPKVLPKIAEKPSKPADKAPLASPEPVSETEASLKINPAAMLPGAVPTLPGAISVFPGMDPSSSSGVSSSSLSPSPVTTPVGAQTDSEGVTFDTPVQVTTLQSVQKSRTKGAGQRRPQTRAARQKAVQRSVDDRDNFTSTAASGPNPNLSDSESRLPERASQTSSSSTISTSSTPTVLAASPPSQHTVLKGSPRPSVLSLPESNDALKKESSKPSGKVLAYSDVDDIFASDVLFEATSVTNTPPTVLSTAKTPPVGSGVLAAKKDKDKSTFQSIFDDDVDDLFQPAKPRSTAKKAKQSKFLEDDDDDEDIFGVSNSSTPSSTSSKEMKNGSSFSKQEIFQDEVTTVPKVQKKHKEKPLDFSLFDDDTDIFADLTDTFKPKQKLKTKPEAKSIFDDDMDDIFSSSKVKKVSKASNKSKKTIPAQDTTAATDSSNIFDDPLNALGGT

Target ORF information:

RefSeq Version	XM_008321893.3
Organism	Cynoglossus semilaevis(tongue sole)
Definition	Cynoglossus semilaevis WASH complex subunit 2-like (LOC103387313), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008321893.3

ORF Insert Sequence:

ATGAGCGGTT TTCCGGGGGG AATAGCAAAT GGTCCCAGCC AGAGTGAACA CATGGAGAAA 
GATGCCCGGG TATGGGAGAG ACCCTGGACG TTGGAAGAAA TGCGGCAGAG TAGTGCCAGC 
TGGTCCTTGG CAGCAGATTC AGGGCTCTTC CTGTTTCTGC AAGACTTCTC TCACAAAATG 
CTGTCAAAGA CGCATGAGAT TGAAAAACAA TTGGATGGCC TCATCCGTGA CACCAAGGCT 
ACAGACAGCT GCCTGCACTC TGTGTTCAGT GACTTCCTCA TGCTCTCCAA TACACAGTTT 
ATAGAAAATA GAGTATGTGA TGAAGAAGTC GAAGAACCAC TGCCTAAAAC TGATGCTGTG 
GAGAAACAAC CGGAACGGAC CCGGGAGCAA AAGGAGGCGG AGCTGATCCC TAAGATGCAG 
GAAGCTGTAA ATTACGGTCT ACGAGTGCTG GAGTCGGCGT TTGAGCATCT GGACATCAAG 
GCAGGGAACT CGGATTCAGA GGATGAGGAG GTCACGGGCA GAGTAGAGGC CATCCTGGAA 
CCTAAGGATC TTTATGTGGA CAGACCACTT CCCTATCTGA TTGGTTCCCA GGCGTTCATG 
GAACAGGATG ATGTTGGACT TGGCGACCTC TCCAGTGATG AAATGTCAGT AGACAGTGAC 
AGAGACAGCG TGATTGAAAG TGACGACGGC AAAGAAGCCA CACATTCGGA TGAAGATTTT 
ATTCAAGAAG GCCAGGTTCA CAACATCTTC AAAAAGAAAT CATCTATGTT GAGCTTCGAA 
GATGACGACG AGGAAGAGGA AGACGAGGAT TCGGACATAT TTGGAGAATC AGACAAGGAT 
GATGACGACC ACGACCCGAA GAACTCGGGT CCAATATCAT TTGCTGATGA GCTGGCAGCT 
CGGATCAGAG GTGAACCAGT TCATAAACAG GAGGGAGATC GTGCCTCATT GTCATCTAAG 
AAGAAAAGTA AAGGCAGAAA AGAAGCAAAG CCCTCAAAGA CCCAGGCTGA AGATGACAGT 
GACGACATGT TCAAACCACC CAAGATGGAT GAGATCGACG ATGACGATGA TGACGATGAC 
TTTTCCCCGT TTGGAGGAAG TAGCCGCCTC TTTAGTGGGG GGAAGGGCCT GTTTGATGAT 
GACAACGACG AGCGGGACCT TTTCGATGAT GCACCAAAAC CTTTTGTACC AGAAGAAAAG 
AAACCTGTAA AAGAAAGTGT GAAAGTCACA GCCACAACAG CAGAACCTAG GAAATCTGCA 
AAGAAGATTC CAACAGGTGG CGTTTCAATA TTTCCAGCCA ACAGCCTCTT CAGTCCAGGG 
AATGATTCTG ATTCTGTTAG AAGCAAGGAG AACGGAACTC CGAATTCCAA GGTCAGCGCT 
CCTCCAAAAG TTGAAAAAAA GACTGCTGTT GGTGGTGGAG GACTCTTTGA TGAGGAGGAT 
GAGGACGACG ACTTCTTTGG TGGAAAAAGT CTACAAAAGT CTGAATCTGT TGTAAAGGAG 
AAACCTAAAT CCAAGACAAC CGTTGACCTC TTTGATGAAG ATGATGAGGA TGATGGCAAT 
ATATTCGGTG ATAAATTTAA GTCAACAACT CCAGTTCAGG TCAAGAAGGA GGTTGTTGCA 
GAACCAGTTA AACCGCCTGA GAAGAAGATG CCAGCTGGGG CCATCTCCAT GTTTGGACCT 
GGAACTAAAA GTCTCCTCAG CGAAGGCCTG AAAAAGCGTC GACAATCGAC CAGTGAGGAG 
TCGGAAAAAT CTGAGGAGAA CGGTGCTTCT CCAGATGTTA GGAAAGCTGC TCCAAAACCA 
TCCCCGAAAC CCCAGACCAG GGGTCTTTTC TCTGATGATG AAGATGCACA AGCTTTTCCA 
ACCATCCCAA GAAGTCAATC GAAGCCTGAA CCTACAAACC AGAGCAACAC CAACAAAGCT 
CCTGTGTTCT TGTTTGATGA CGATGAAGAA GAGGATCTCT TTGCTTCTGC GGTTAAATCT 
AAACCAAAAG CTAGTTCATC TAAAACCTCA CCCATGCAAA CCAGCAGTGC AGTCTCTAGC 
TCCCTCTTCA GTGATGATGA GGATCAGTGG TTAGGTGCTA CAAAAACATC AGAGACCAAC 
TCAGGAGGAA TGAAGGCCAG TGCTAGCGCC CCCTCTGGTC TCACTAGTAC CAAAACAACA 
CCCAGCAGCA GCCTGTTTGA GGAGGAAGAT GATAATGAAC TGTTTGCACA AACAAAAGAA 
TCAAGTACTC AGAAAAACCC TCAGCCAGTT GCACTACTGT TTGAAGATAA TGGTGGTGAT 
GATGAAGATG ACGAAGATCA AGGAACCCTT TTTAATGTCA ACACAAATAC TGCACTACCA 
ACTACTAAAA CACTAGCCTC AGCATCCAAG TCTTCCTCAT CCCAGATGCC TAAATCAGAC 
ACAGTAGAAC ATGTACCCAC AGAAAACCAG TCTTTGCAGC AGGAGAAGCT GACTCCACAT 
ACTTCAAAGT CTCCAGAGAT GCCTGTGGGA GAAAGTGGAG AAAGTAAAAA GAAGCCTGCT 
AGGGCTGTTA GTCTCTTCGG AGGCATTAAT GTTCTTGAAA AGCAGACAAA AACCCTAATA 
CAGGATGAAG AAGAAGAAGA AGATGATGAC TTCCCTTTGA AGCCCAGCCC TCCCCCATTT 
GTCATGGAGG AGAAGGAAGA AAAATCTAGA TCAGGGGCTT TTGGTCTGTT TGAAGATGAA 
GACGACGACG AGTGGAATGA TTCTATTTTC ACACCTGGTA AACCCACCAG CAAAACAACA 
CCCAAGGAAG AAGAAGAGGA GGAGGAGGAG GAGGCGGCGG AGGCGGAGGA GAAAGAGGAG 
GTGGAGCCAG CACAGACAAA GAGCACGGGT GTTCTCCAGG ACGAGGAGTT GTTCAGCCAC 
AGTCATGAGA AAGACAATGA CCTCGACGCC GGTCTCTTTG CCACTTCAGG GAGAGCTGAA 
AGCCCACCAA AGGCAGCAGC ACATAATTTG TTTGCAGAAG ACGATGAAGA TGACCTTTTC 
AGCTCCCCCA AGCCAAAAGT TCTTCCAAAA ATTGCAGAAA AGCCAAGTAA ACCTGCTGAC 
AAAGCACCGC TGGCCAGTCC AGAACCTGTC TCAGAGACTG AGGCAAGTCT CAAGATCAAT 
CCTGCTGCTA TGCTTCCTGG TGCTGTTCCC ACTTTGCCAG GTGCCATCAG TGTCTTCCCT 
GGCATGGATC CCAGCTCCTC CTCCGGTGTG TCAAGCTCCA GCCTGAGCCC CAGCCCTGTG 
ACAACTCCTG TAGGGGCTCA GACAGACAGT GAGGGAGTGA CCTTTGACAC CCCAGTCCAG 
GTCACGACAC TGCAGAGTGT ACAGAAAAGT CGCACCAAAG GTGCTGGTCA GCGCAGACCC 
CAGACCAGAG CTGCAAGACA GAAAGCTGTA CAAAGATCAG TCGACGACAG AGACAATTTC 
ACAAGTACAG CTGCGTCAGG TCCAAACCCC AATCTGTCTG ATTCAGAGTC ACGTCTACCA 
GAGAGAGCCA GCCAGACGAG TAGCAGTTCA ACGATTTCCA CCTCTTCCAC GCCCACAGTC 
TTGGCCGCCT CCCCACCTTC TCAGCACACT GTCCTGAAAG GATCCCCCAG ACCTTCTGTT 
TTGTCATTGC CAGAGTCGAA TGATGCTCTA AAGAAAGAGT CCAGTAAACC CAGTGGTAAA 
GTGTTGGCAT ACTCTGATGT GGATGACATT TTTGCCTCCG ACGTTCTGTT TGAGGCCACG 
TCGGTGACAA ACACACCCCC CACCGTACTG TCAACCGCGA AGACACCACC TGTAGGCAGC 
GGCGTGTTGG CTGCTAAAAA AGACAAAGAC AAAAGCACAT TCCAGTCTAT TTTTGATGAC 
GATGTGGATG ATTTGTTTCA ACCAGCCAAA CCGAGGTCGA CGGCCAAGAA AGCCAAGCAG 
TCGAAATTCT TGGAAGACGA CGATGATGAC GAGGACATAT TCGGTGTCAG CAACAGTTCC 
ACTCCGTCAT CGACCAGTAG TAAAGAAATG AAGAATGGTA GTAGTTTTTC CAAGCAGGAA 
ATTTTCCAGG ACGAAGTAAC TACTGTGCCT AAAGTTCAAA AGAAGCACAA AGAGAAGCCT 
TTAGATTTCA GCTTGTTCGA TGATGACACA GACATTTTTG CTGATCTGAC AGACACTTTC 
AAACCAAAGC AGAAACTCAA GACAAAGCCA GAGGCCAAGT CGATATTTGA CGATGACATG 
GATGACATCT TCTCATCGAG CAAAGTGAAG AAAGTTTCAA AGGCTTCAAA TAAATCTAAG 
AAAACGATAC CCGCCCAGGA CACGACTGCA GCGACAGATT CCAGCAACAT ATTTGACGAT 
CCACTAAACG CCCTTGGTGG GACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103387313 ( XM_008321893.2 ) cDNA ORF clone, Cynoglossus semilaevis(tongue sole) -> XP_008320115.1 Cynoglossus semilaevis WASH complex subunit FAM21-like (LOC103387313), transcript variant X2, mRNA.

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CloneID	OCy39599
Clone ID Related Accession (Same CDS sequence)	XM_008321893.3 , XM_008321893.2
Accession Version	XM_008321893.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4347bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-06
Organism	Cynoglossus semilaevis(tongue sole)
Product	WASH complex subunit FAM21-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024318.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 7, 2016 this sequence version replaced XM_008321893.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cynoglossus semilaevis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGTT TTCCGGGGGG AATAGCAAAT GGTCCCAGCC AGAGTGAACA CATGGAGAAA 
GATGCCCGGG TATGGGAGAG ACCCTGGACG TTGGAAGAAA TGCGGCAGAG TAGTGCCAGC 
TGGTCCTTGG CAGCAGATTC AGGGCTCTTC CTGTTTCTGC AAGACTTCTC TCACAAAATG 
CTGTCAAAGA CGCATGAGAT TGAAAAACAA TTGGATGGCC TCATCCGTGA CACCAAGGCT 
ACAGACAGCT GCCTGCACTC TGTGTTCAGT GACTTCCTCA TGCTCTCCAA TACACAGTTT 
ATAGAAAATA GAGTATGTGA TGAAGAAGTC GAAGAACCAC TGCCTAAAAC TGATGCTGTG 
GAGAAACAAC CGGAACGGAC CCGGGAGCAA AAGGAGGCGG AGCTGATCCC TAAGATGCAG 
GAAGCTGTAA ATTACGGTCT ACGAGTGCTG GAGTCGGCGT TTGAGCATCT GGACATCAAG 
GCAGGGAACT CGGATTCAGA GGATGAGGAG GTCACGGGCA GAGTAGAGGC CATCCTGGAA 
CCTAAGGATC TTTATGTGGA CAGACCACTT CCCTATCTGA TTGGTTCCCA GGCGTTCATG 
GAACAGGATG ATGTTGGACT TGGCGACCTC TCCAGTGATG AAATGTCAGT AGACAGTGAC 
AGAGACAGCG TGATTGAAAG TGACGACGGC AAAGAAGCCA CACATTCGGA TGAAGATTTT 
ATTCAAGAAG GCCAGGTTCA CAACATCTTC AAAAAGAAAT CATCTATGTT GAGCTTCGAA 
GATGACGACG AGGAAGAGGA AGACGAGGAT TCGGACATAT TTGGAGAATC AGACAAGGAT 
GATGACGACC ACGACCCGAA GAACTCGGGT CCAATATCAT TTGCTGATGA GCTGGCAGCT 
CGGATCAGAG GTGAACCAGT TCATAAACAG GAGGGAGATC GTGCCTCATT GTCATCTAAG 
AAGAAAAGTA AAGGCAGAAA AGAAGCAAAG CCCTCAAAGA CCCAGGCTGA AGATGACAGT 
GACGACATGT TCAAACCACC CAAGATGGAT GAGATCGACG ATGACGATGA TGACGATGAC 
TTTTCCCCGT TTGGAGGAAG TAGCCGCCTC TTTAGTGGGG GGAAGGGCCT GTTTGATGAT 
GACAACGACG AGCGGGACCT TTTCGATGAT GCACCAAAAC CTTTTGTACC AGAAGAAAAG 
AAACCTGTAA AAGAAAGTGT GAAAGTCACA GCCACAACAG CAGAACCTAG GAAATCTGCA 
AAGAAGATTC CAACAGGTGG CGTTTCAATA TTTCCAGCCA ACAGCCTCTT CAGTCCAGGG 
AATGATTCTG ATTCTGTTAG AAGCAAGGAG AACGGAACTC CGAATTCCAA GGTCAGCGCT 
CCTCCAAAAG TTGAAAAAAA GACTGCTGTT GGTGGTGGAG GACTCTTTGA TGAGGAGGAT 
GAGGACGACG ACTTCTTTGG TGGAAAAAGT CTACAAAAGT CTGAATCTGT TGTAAAGGAG 
AAACCTAAAT CCAAGACAAC CGTTGACCTC TTTGATGAAG ATGATGAGGA TGATGGCAAT 
ATATTCGGTG ATAAATTTAA GTCAACAACT CCAGTTCAGG TCAAGAAGGA GGTTGTTGCA 
GAACCAGTTA AACCGCCTGA GAAGAAGATG CCAGCTGGGG CCATCTCCAT GTTTGGACCT 
GGAACTAAAA GTCTCCTCAG CGAAGGCCTG AAAAAGCGTC GACAATCGAC CAGTGAGGAG 
TCGGAAAAAT CTGAGGAGAA CGGTGCTTCT CCAGATGTTA GGAAAGCTGC TCCAAAACCA 
TCCCCGAAAC CCCAGACCAG GGGTCTTTTC TCTGATGATG AAGATGCACA AGCTTTTCCA 
ACCATCCCAA GAAGTCAATC GAAGCCTGAA CCTACAAACC AGAGCAACAC CAACAAAGCT 
CCTGTGTTCT TGTTTGATGA CGATGAAGAA GAGGATCTCT TTGCTTCTGC GGTTAAATCT 
AAACCAAAAG CTAGTTCATC TAAAACCTCA CCCATGCAAA CCAGCAGTGC AGTCTCTAGC 
TCCCTCTTCA GTGATGATGA GGATCAGTGG TTAGGTGCTA CAAAAACATC AGAGACCAAC 
TCAGGAGGAA TGAAGGCCAG TGCTAGCGCC CCCTCTGGTC TCACTAGTAC CAAAACAACA 
CCCAGCAGCA GCCTGTTTGA GGAGGAAGAT GATAATGAAC TGTTTGCACA AACAAAAGAA 
TCAAGTACTC AGAAAAACCC TCAGCCAGTT GCACTACTGT TTGAAGATAA TGGTGGTGAT 
GATGAAGATG ACGAAGATCA AGGAACCCTT TTTAATGTCA ACACAAATAC TGCACTACCA 
ACTACTAAAA CACTAGCCTC AGCATCCAAG TCTTCCTCAT CCCAGATGCC TAAATCAGAC 
ACAGTAGAAC ATGTACCCAC AGAAAACCAG TCTTTGCAGC AGGAGAAGCT GACTCCACAT 
ACTTCAAAGT CTCCAGAGAT GCCTGTGGGA GAAAGTGGAG AAAGTAAAAA GAAGCCTGCT 
AGGGCTGTTA GTCTCTTCGG AGGCATTAAT GTTCTTGAAA AGCAGACAAA AACCCTAATA 
CAGGATGAAG AAGAAGAAGA AGATGATGAC TTCCCTTTGA AGCCCAGCCC TCCCCCATTT 
GTCATGGAGG AGAAGGAAGA AAAATCTAGA TCAGGGGCTT TTGGTCTGTT TGAAGATGAA 
GACGACGACG AGTGGAATGA TTCTATTTTC ACACCTGGTA AACCCACCAG CAAAACAACA 
CCCAAGGAAG AAGAAGAGGA GGAGGAGGAG GAGGCGGCGG AGGCGGAGGA GAAAGAGGAG 
GTGGAGCCAG CACAGACAAA GAGCACGGGT GTTCTCCAGG ACGAGGAGTT GTTCAGCCAC 
AGTCATGAGA AAGACAATGA CCTCGACGCC GGTCTCTTTG CCACTTCAGG GAGAGCTGAA 
AGCCCACCAA AGGCAGCAGC ACATAATTTG TTTGCAGAAG ACGATGAAGA TGACCTTTTC 
AGCTCCCCCA AGCCAAAAGT TCTTCCAAAA ATTGCAGAAA AGCCAAGTAA ACCTGCTGAC 
AAAGCACCGC TGGCCAGTCC AGAACCTGTC TCAGAGACTG AGGCAAGTCT CAAGATCAAT 
CCTGCTGCTA TGCTTCCTGG TGCTGTTCCC ACTTTGCCAG GTGCCATCAG TGTCTTCCCT 
GGCATGGATC CCAGCTCCTC CTCCGGTGTG TCAAGCTCCA GCCTGAGCCC CAGCCCTGTG 
ACAACTCCTG TAGGGGCTCA GACAGACAGT GAGGGAGTGA CCTTTGACAC CCCAGTCCAG 
GTCACGACAC TGCAGAGTGT ACAGAAAAGT CGCACCAAAG GTGCTGGTCA GCGCAGACCC 
CAGACCAGAG CTGCAAGACA GAAAGCTGTA CAAAGATCAG TCGACGACAG AGACAATTTC 
ACAAGTACAG CTGCGTCAGG TCCAAACCCC AATCTGTCTG ATTCAGAGTC ACGTCTACCA 
GAGAGAGCCA GCCAGACGAG TAGCAGTTCA ACGATTTCCA CCTCTTCCAC GCCCACAGTC 
TTGGCCGCCT CCCCACCTTC TCAGCACACT GTCCTGAAAG GATCCCCCAG ACCTTCTGTT 
TTGTCATTGC CAGAGTCGAA TGATGCTCTA AAGAAAGAGT CCAGTAAACC CAGTGGTAAA 
GTGTTGGCAT ACTCTGATGT GGATGACATT TTTGCCTCCG ACGTTCTGTT TGAGGCCACG 
TCGGTGACAA ACACACCCCC CACCGTACTG TCAACCGCGA AGACACCACC TGTAGGCAGC 
GGCGTGTTGG CTGCTAAAAA AGACAAAGAC AAAAGCACAT TCCAGTCTAT TTTTGATGAC 
GATGTGGATG ATTTGTTTCA ACCAGCCAAA CCGAGGTCGA CGGCCAAGAA AGCCAAGCAG 
TCGAAATTCT TGGAAGACGA CGATGATGAC GAGGACATAT TCGGTGTCAG CAACAGTTCC 
ACTCCGTCAT CGACCAGTAG TAAAGAAATG AAGAATGGTA GTAGTTTTTC CAAGCAGGAA 
ATTTTCCAGG ACGAAGTAAC TACTGTGCCT AAAGTTCAAA AGAAGCACAA AGAGAAGCCT 
TTAGATTTCA GCTTGTTCGA TGATGACACA GACATTTTTG CTGATCTGAC AGACACTTTC 
AAACCAAAGC AGAAACTCAA GACAAAGCCA GAGGCCAAGT CGATATTTGA CGATGACATG 
GATGACATCT TCTCATCGAG CAAAGTGAAG AAAGTTTCAA AGGCTTCAAA TAAATCTAAG 
AAAACGATAC CCGCCCAGGA CACGACTGCA GCGACAGATT CCAGCAACAT ATTTGACGAT 
CCACTAAACG CCCTTGGTGG GACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008320115.1
CDS	141..4487
Translation	MSGFPGGIANGPSQSEHMEKDARVWERPWTLEEMRQSSASWSLAADSGLFLFLQDFSHKMLSKTHEIEKQLDGLIRDTKATDSCLHSVFSDFLMLSNTQFIENRVCDEEVEEPLPKTDAVEKQPERTREQKEAELIPKMQEAVNYGLRVLESAFEHLDIKAGNSDSEDEEVTGRVEAILEPKDLYVDRPLPYLIGSQAFMEQDDVGLGDLSSDEMSVDSDRDSVIESDDGKEATHSDEDFIQEGQVHNIFKKKSSMLSFEDDDEEEEDEDSDIFGESDKDDDDHDPKNSGPISFADELAARIRGEPVHKQEGDRASLSSKKKSKGRKEAKPSKTQAEDDSDDMFKPPKMDEIDDDDDDDDFSPFGGSSRLFSGGKGLFDDDNDERDLFDDAPKPFVPEEKKPVKESVKVTATTAEPRKSAKKIPTGGVSIFPANSLFSPGNDSDSVRSKENGTPNSKVSAPPKVEKKTAVGGGGLFDEEDEDDDFFGGKSLQKSESVVKEKPKSKTTVDLFDEDDEDDGNIFGDKFKSTTPVQVKKEVVAEPVKPPEKKMPAGAISMFGPGTKSLLSEGLKKRRQSTSEESEKSEENGASPDVRKAAPKPSPKPQTRGLFSDDEDAQAFPTIPRSQSKPEPTNQSNTNKAPVFLFDDDEEEDLFASAVKSKPKASSSKTSPMQTSSAVSSSLFSDDEDQWLGATKTSETNSGGMKASASAPSGLTSTKTTPSSSLFEEEDDNELFAQTKESSTQKNPQPVALLFEDNGGDDEDDEDQGTLFNVNTNTALPTTKTLASASKSSSSQMPKSDTVEHVPTENQSLQQEKLTPHTSKSPEMPVGESGESKKKPARAVSLFGGINVLEKQTKTLIQDEEEEEDDDFPLKPSPPPFVMEEKEEKSRSGAFGLFEDEDDDEWNDSIFTPGKPTSKTTPKEEEEEEEEEAAEAEEKEEVEPAQTKSTGVLQDEELFSHSHEKDNDLDAGLFATSGRAESPPKAAAHNLFAEDDEDDLFSSPKPKVLPKIAEKPSKPADKAPLASPEPVSETEASLKINPAAMLPGAVPTLPGAISVFPGMDPSSSSGVSSSSLSPSPVTTPVGAQTDSEGVTFDTPVQVTTLQSVQKSRTKGAGQRRPQTRAARQKAVQRSVDDRDNFTSTAASGPNPNLSDSESRLPERASQTSSSSTISTSSTPTVLAASPPSQHTVLKGSPRPSVLSLPESNDALKKESSKPSGKVLAYSDVDDIFASDVLFEATSVTNTPPTVLSTAKTPPVGSGVLAAKKDKDKSTFQSIFDDDVDDLFQPAKPRSTAKKAKQSKFLEDDDDDEDIFGVSNSSTPSSTSSKEMKNGSSFSKQEIFQDEVTTVPKVQKKHKEKPLDFSLFDDDTDIFADLTDTFKPKQKLKTKPEAKSIFDDDMDDIFSSSKVKKVSKASNKSKKTIPAQDTTAATDSSNIFDDPLNALGGT

Target ORF information:

RefSeq Version	XM_008321893.2
Organism	Cynoglossus semilaevis(tongue sole)
Definition	Cynoglossus semilaevis WASH complex subunit FAM21-like (LOC103387313), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008321893.2

ORF Insert Sequence:

ATGAGCGGTT TTCCGGGGGG AATAGCAAAT GGTCCCAGCC AGAGTGAACA CATGGAGAAA 
GATGCCCGGG TATGGGAGAG ACCCTGGACG TTGGAAGAAA TGCGGCAGAG TAGTGCCAGC 
TGGTCCTTGG CAGCAGATTC AGGGCTCTTC CTGTTTCTGC AAGACTTCTC TCACAAAATG 
CTGTCAAAGA CGCATGAGAT TGAAAAACAA TTGGATGGCC TCATCCGTGA CACCAAGGCT 
ACAGACAGCT GCCTGCACTC TGTGTTCAGT GACTTCCTCA TGCTCTCCAA TACACAGTTT 
ATAGAAAATA GAGTATGTGA TGAAGAAGTC GAAGAACCAC TGCCTAAAAC TGATGCTGTG 
GAGAAACAAC CGGAACGGAC CCGGGAGCAA AAGGAGGCGG AGCTGATCCC TAAGATGCAG 
GAAGCTGTAA ATTACGGTCT ACGAGTGCTG GAGTCGGCGT TTGAGCATCT GGACATCAAG 
GCAGGGAACT CGGATTCAGA GGATGAGGAG GTCACGGGCA GAGTAGAGGC CATCCTGGAA 
CCTAAGGATC TTTATGTGGA CAGACCACTT CCCTATCTGA TTGGTTCCCA GGCGTTCATG 
GAACAGGATG ATGTTGGACT TGGCGACCTC TCCAGTGATG AAATGTCAGT AGACAGTGAC 
AGAGACAGCG TGATTGAAAG TGACGACGGC AAAGAAGCCA CACATTCGGA TGAAGATTTT 
ATTCAAGAAG GCCAGGTTCA CAACATCTTC AAAAAGAAAT CATCTATGTT GAGCTTCGAA 
GATGACGACG AGGAAGAGGA AGACGAGGAT TCGGACATAT TTGGAGAATC AGACAAGGAT 
GATGACGACC ACGACCCGAA GAACTCGGGT CCAATATCAT TTGCTGATGA GCTGGCAGCT 
CGGATCAGAG GTGAACCAGT TCATAAACAG GAGGGAGATC GTGCCTCATT GTCATCTAAG 
AAGAAAAGTA AAGGCAGAAA AGAAGCAAAG CCCTCAAAGA CCCAGGCTGA AGATGACAGT 
GACGACATGT TCAAACCACC CAAGATGGAT GAGATCGACG ATGACGATGA TGACGATGAC 
TTTTCCCCGT TTGGAGGAAG TAGCCGCCTC TTTAGTGGGG GGAAGGGCCT GTTTGATGAT 
GACAACGACG AGCGGGACCT TTTCGATGAT GCACCAAAAC CTTTTGTACC AGAAGAAAAG 
AAACCTGTAA AAGAAAGTGT GAAAGTCACA GCCACAACAG CAGAACCTAG GAAATCTGCA 
AAGAAGATTC CAACAGGTGG CGTTTCAATA TTTCCAGCCA ACAGCCTCTT CAGTCCAGGG 
AATGATTCTG ATTCTGTTAG AAGCAAGGAG AACGGAACTC CGAATTCCAA GGTCAGCGCT 
CCTCCAAAAG TTGAAAAAAA GACTGCTGTT GGTGGTGGAG GACTCTTTGA TGAGGAGGAT 
GAGGACGACG ACTTCTTTGG TGGAAAAAGT CTACAAAAGT CTGAATCTGT TGTAAAGGAG 
AAACCTAAAT CCAAGACAAC CGTTGACCTC TTTGATGAAG ATGATGAGGA TGATGGCAAT 
ATATTCGGTG ATAAATTTAA GTCAACAACT CCAGTTCAGG TCAAGAAGGA GGTTGTTGCA 
GAACCAGTTA AACCGCCTGA GAAGAAGATG CCAGCTGGGG CCATCTCCAT GTTTGGACCT 
GGAACTAAAA GTCTCCTCAG CGAAGGCCTG AAAAAGCGTC GACAATCGAC CAGTGAGGAG 
TCGGAAAAAT CTGAGGAGAA CGGTGCTTCT CCAGATGTTA GGAAAGCTGC TCCAAAACCA 
TCCCCGAAAC CCCAGACCAG GGGTCTTTTC TCTGATGATG AAGATGCACA AGCTTTTCCA 
ACCATCCCAA GAAGTCAATC GAAGCCTGAA CCTACAAACC AGAGCAACAC CAACAAAGCT 
CCTGTGTTCT TGTTTGATGA CGATGAAGAA GAGGATCTCT TTGCTTCTGC GGTTAAATCT 
AAACCAAAAG CTAGTTCATC TAAAACCTCA CCCATGCAAA CCAGCAGTGC AGTCTCTAGC 
TCCCTCTTCA GTGATGATGA GGATCAGTGG TTAGGTGCTA CAAAAACATC AGAGACCAAC 
TCAGGAGGAA TGAAGGCCAG TGCTAGCGCC CCCTCTGGTC TCACTAGTAC CAAAACAACA 
CCCAGCAGCA GCCTGTTTGA GGAGGAAGAT GATAATGAAC TGTTTGCACA AACAAAAGAA 
TCAAGTACTC AGAAAAACCC TCAGCCAGTT GCACTACTGT TTGAAGATAA TGGTGGTGAT 
GATGAAGATG ACGAAGATCA AGGAACCCTT TTTAATGTCA ACACAAATAC TGCACTACCA 
ACTACTAAAA CACTAGCCTC AGCATCCAAG TCTTCCTCAT CCCAGATGCC TAAATCAGAC 
ACAGTAGAAC ATGTACCCAC AGAAAACCAG TCTTTGCAGC AGGAGAAGCT GACTCCACAT 
ACTTCAAAGT CTCCAGAGAT GCCTGTGGGA GAAAGTGGAG AAAGTAAAAA GAAGCCTGCT 
AGGGCTGTTA GTCTCTTCGG AGGCATTAAT GTTCTTGAAA AGCAGACAAA AACCCTAATA 
CAGGATGAAG AAGAAGAAGA AGATGATGAC TTCCCTTTGA AGCCCAGCCC TCCCCCATTT 
GTCATGGAGG AGAAGGAAGA AAAATCTAGA TCAGGGGCTT TTGGTCTGTT TGAAGATGAA 
GACGACGACG AGTGGAATGA TTCTATTTTC ACACCTGGTA AACCCACCAG CAAAACAACA 
CCCAAGGAAG AAGAAGAGGA GGAGGAGGAG GAGGCGGCGG AGGCGGAGGA GAAAGAGGAG 
GTGGAGCCAG CACAGACAAA GAGCACGGGT GTTCTCCAGG ACGAGGAGTT GTTCAGCCAC 
AGTCATGAGA AAGACAATGA CCTCGACGCC GGTCTCTTTG CCACTTCAGG GAGAGCTGAA 
AGCCCACCAA AGGCAGCAGC ACATAATTTG TTTGCAGAAG ACGATGAAGA TGACCTTTTC 
AGCTCCCCCA AGCCAAAAGT TCTTCCAAAA ATTGCAGAAA AGCCAAGTAA ACCTGCTGAC 
AAAGCACCGC TGGCCAGTCC AGAACCTGTC TCAGAGACTG AGGCAAGTCT CAAGATCAAT 
CCTGCTGCTA TGCTTCCTGG TGCTGTTCCC ACTTTGCCAG GTGCCATCAG TGTCTTCCCT 
GGCATGGATC CCAGCTCCTC CTCCGGTGTG TCAAGCTCCA GCCTGAGCCC CAGCCCTGTG 
ACAACTCCTG TAGGGGCTCA GACAGACAGT GAGGGAGTGA CCTTTGACAC CCCAGTCCAG 
GTCACGACAC TGCAGAGTGT ACAGAAAAGT CGCACCAAAG GTGCTGGTCA GCGCAGACCC 
CAGACCAGAG CTGCAAGACA GAAAGCTGTA CAAAGATCAG TCGACGACAG AGACAATTTC 
ACAAGTACAG CTGCGTCAGG TCCAAACCCC AATCTGTCTG ATTCAGAGTC ACGTCTACCA 
GAGAGAGCCA GCCAGACGAG TAGCAGTTCA ACGATTTCCA CCTCTTCCAC GCCCACAGTC 
TTGGCCGCCT CCCCACCTTC TCAGCACACT GTCCTGAAAG GATCCCCCAG ACCTTCTGTT 
TTGTCATTGC CAGAGTCGAA TGATGCTCTA AAGAAAGAGT CCAGTAAACC CAGTGGTAAA 
GTGTTGGCAT ACTCTGATGT GGATGACATT TTTGCCTCCG ACGTTCTGTT TGAGGCCACG 
TCGGTGACAA ACACACCCCC CACCGTACTG TCAACCGCGA AGACACCACC TGTAGGCAGC 
GGCGTGTTGG CTGCTAAAAA AGACAAAGAC AAAAGCACAT TCCAGTCTAT TTTTGATGAC 
GATGTGGATG ATTTGTTTCA ACCAGCCAAA CCGAGGTCGA CGGCCAAGAA AGCCAAGCAG 
TCGAAATTCT TGGAAGACGA CGATGATGAC GAGGACATAT TCGGTGTCAG CAACAGTTCC 
ACTCCGTCAT CGACCAGTAG TAAAGAAATG AAGAATGGTA GTAGTTTTTC CAAGCAGGAA 
ATTTTCCAGG ACGAAGTAAC TACTGTGCCT AAAGTTCAAA AGAAGCACAA AGAGAAGCCT 
TTAGATTTCA GCTTGTTCGA TGATGACACA GACATTTTTG CTGATCTGAC AGACACTTTC 
AAACCAAAGC AGAAACTCAA GACAAAGCCA GAGGCCAAGT CGATATTTGA CGATGACATG 
GATGACATCT TCTCATCGAG CAAAGTGAAG AAAGTTTCAA AGGCTTCAAA TAAATCTAAG 
AAAACGATAC CCGCCCAGGA CACGACTGCA GCGACAGATT CCAGCAACAT ATTTGACGAT 
CCACTAAACG CCCTTGGTGG GACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103387313 ( XM_017037518.1 ) cDNA ORF clone, Cynoglossus semilaevis(tongue sole) -> XP_016893007.1 Cynoglossus semilaevis WASH complex subunit FAM21-like (LOC103387313), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCy39600
Clone ID Related Accession (Same CDS sequence)	XM_017037518.1
Accession Version	XM_017037518.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4347bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-06
Organism	Cynoglossus semilaevis(tongue sole)
Product	WASH complex subunit FAM21-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024318.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cynoglossus semilaevis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGTT TTCCGGGGGG AATAGCAAAT GGTCCCAGCC AGAGTGAACA CATGGAGAAA 
GATGCCCGGG TATGGGAGAG ACCCTGGACG TTGGAAGAAA TGCGGCAGAG TAGTGCCAGC 
TGGTCCTTGG CAGCAGATTC AGGGCTCTTC CTGTTTCTGC AAGACTTCTC TCACAAAATG 
CTGTCAAAGA CGCATGAGAT TGAAAAACAA TTGGATGGCC TCATCCGTGA CACCAAGGCT 
ACAGACAGCT GCCTGCACTC TGTGTTCAGT GACTTCCTCA TGCTCTCCAA TACACAGTTT 
ATAGAAAATA GAGTATGTGA TGAAGAAGTC GAAGAACCAC TGCCTAAAAC TGATGCTGTG 
GAGAAACAAC CGGAACGGAC CCGGGAGCAA AAGGAGGCGG AGCTGATCCC TAAGATGCAG 
GAAGCTGTAA ATTACGGTCT ACGAGTGCTG GAGTCGGCGT TTGAGCATCT GGACATCAAG 
GCAGGGAACT CGGATTCAGA GGATGAGGAG GTCACGGGCA GAGTAGAGGC CATCCTGGAA 
CCTAAGGATC TTTATGTGGA CAGACCACTT CCCTATCTGA TTGGTTCCCA GGCGTTCATG 
GAACAGGATG ATGTTGGACT TGGCGACCTC TCCAGTGATG AAATGTCAGT AGACAGTGAC 
AGAGACAGCG TGATTGAAAG TGACGACGGC AAAGAAGCCA CACATTCGGA TGAAGATTTT 
ATTCAAGAAG GCCAGGTTCA CAACATCTTC AAAAAGAAAT CATCTATGTT GAGCTTCGAA 
GATGACGACG AGGAAGAGGA AGACGAGGAT TCGGACATAT TTGGAGAATC AGACAAGGAT 
GATGACGACC ACGACCCGAA GAACTCGGGT CCAATATCAT TTGCTGATGA GCTGGCAGCT 
CGGATCAGAG GTGAACCAGT TCATAAACAG GAGGGAGATC GTGCCTCTGA AGATGACAGT 
GACGACATGT TCAAACCACC CAAGATGGAT GAGATCGACG ATGACGATGA TGACGATGAC 
TTTTCCCCGT TTGGAGGAAG TAGCCGCCTC TTTAGTGGGG GGAAGGGCCT GTTTGATGAT 
GACAACGACG AGCGGGACCT TTTCGATGAT GCACCAAAAC CTTTTGTACC AGAAGAAAAG 
AAACCTGTAA AAGAAAGTGT GAAAGTCACA GCCACAACAG CAGAACCTAG GAAATCTGCA 
AAGAAGATTC CAACAGGTGG CGTTTCAATA TTTCCAGCCA ACAGCCTCTT CAGTCCAGGG 
AATGATTCTG ATTCTGTTAG AAGCAAGGAG AACGGAACTC CGAATTCCAA GGTCAGCGCT 
CCTCCAAAAG TTGAAAAAAA GACTGCTGTT GGTGGTGGAG GACTCTTTGA TGAGGAGGAT 
GAGGACGACG ACTTCTTTGG TGGAAAAAGT CTACAAAAGT CTGAATCTGT TGTAAAGGAG 
AAACCTAAAT CCAAGACAAC CGTTGACCTC TTTGATGAAG ATGATGAGGA TGATGGCAAT 
ATATTCGGTG ATAAATTTAA GTCAACAACT CCAGTTCAGG TCAAGAAGGA GGTTGTTGCA 
GAACCAGTTA AACCGCCTGA GAAGAAGATG CCAGCTGGGG CCATCTCCAT GTTTGGACCT 
GGAACTAAAA GTCTCCTCAG CGAAGGCCTG AAAAAGCGTC GACAATCGAC CAGTGAGGAG 
TCGGAAAAAT CTGAGGAGAA CGGTGCTTCT CCAGATGTTA GGAAAGCTGC TCCAAAACCA 
TCCCCGAAAC CCCAGACCAG GGGTCTTTTC TCTGATGATG AAGATGCACA AGCTTTTCCA 
ACCATCCCAA GAAGTCAATC GAAGCCTGAA CCTACAAACC AGAGCAACAC CAACAAAGCT 
CCTGTGTTCT TGTTTGATGA CGATGAAGAA GAGGATCTCT TTGCTTCTGC GGTTAAATCT 
AAACCAAAAG CTAGTTCATC TAAAACCTCA CCCATGCAAA CCAGCAGTGC AGTCTCTAGC 
TCCCTCTTCA GTGATGATGA GGATCAGTGG TTAGGTGCTA CAAAAACATC AGAGACCAAC 
TCAGGAGGAA TGAAGGCCAG TGCTAGCGCC CCCTCTGGTC TCACTAGTAC CAAAACAACA 
CCCAGCAGCA GCCTGTTTGA GGAGGAAGAT GATAATGAAC TGTTTGCACA AACAAAAGAA 
TCAAGTACTC AGAAAAACCC TCAGCCAGTT GCACTACTGT TTGAAGATAA TGGTGGTGAT 
GATGAAGATG ACGAAGATCA AGGAACCCTT TTTAATGTCA ACACAAATAC TGCACTACCA 
ACTACTAAAA CACTAGCCTC AGCATCCAAG TCTTCCTCAT CCCAGATGCC TAAATCAGAC 
ACAGTAGAAC ATGTACCCAC AGAAAACCAG TCTTTGCAGC AGGAGAAGCT GACTCCACAT 
ACTTCAAAGT CTCCAGAGAT GCCTGTGGGA GAAAGTGGAG AAAGTAAAAA GAAGCCTGCT 
AGGGCTGTTA GTCTCTTCGG AGGCATTAAT GTTCTTGAAA AGCAGACAAA AACCCTAATA 
CAGGATGAAG AAGAAGAAGA AGATGATGAC TTCCCTTTGA AGCCCAGCCC TCCCCCATTT 
GTCATGGAGG AGAAGGAAGA AAAATCTAGA TCAGGGGCTT TTGGTCTGTT TGAAGATGAA 
GACGACGACG AGTGGAATGA TTCTATTTTC ACACCTGGTA AACCCACCAG CAAAACAACA 
CCCAAGGAAG AAGAAGAGGA GGAGGAGGAG GAGGCGGCGG AGGCGGAGGA GAAAGAGGAG 
GTGGAGCCAG CACAGACAAA GAGCACGGGT GTTCTCCAGG ACGAGGAGTT GTTCAGCCAC 
AGTCATGAGA AAGACAATGA CCTCGACGCC GGTCTCTTTG CCACTTCAGG GAGAGCTGAA 
AGCCCACCAA AGGCAGCAGC ACATAATTTG TTTGCAGAAG ACGATGAAGA TGACCTTTTC 
AGCTCCCCCA AGCCAAAAGT TCTTCCAAAA ATTGCAGAAA AGCCAAGTAA ACCTGCTGAC 
AAAGCACCGC TGGCCAGTCC AGAACCTGTC TCAGAGACTG AGAAACCTGC ACCAAGTCCT 
GTAAAACCTA AAGCTTCCTC ATCAAGGATT GGAAAACTTC AAGCAAGTCT CAAGATCAAT 
CCTGCTGCTA TGCTTCCTGG TGCTGTTCCC ACTTTGCCAG GTGCCATCAG TGTCTTCCCT 
GGCATGGATC CCAGCTCCTC CTCCGGTGTG TCAAGCTCCA GCCTGAGCCC CAGCCCTGTG 
ACAACTCCTG TAGGGGCTCA GACAGACAGT GAGGGAGTGA CCTTTGACAC CCCAGTCCAG 
GTCACGACAC TGCAGAGTGT ACAGAAAAGT CGCACCAAAG GTGCTGGTCA GCGCAGACCC 
CAGACCAGAG CTGCAAGACA GAAAGCTGTA CAAAGATCAG TCGACGACAG AGACAATTTC 
ACAAGTACAG CTGCGTCAGG TCCAAACCCC AATCTGTCTG ATTCAGAGTC ACGTCTACCA 
GAGAGAGCCA GCCAGACGAG TAGCAGTTCA ACGATTTCCA CCTCTTCCAC GCCCACAGTC 
TTGGCCGCCT CCCCACCTTC TCAGCACACT GTCCTGAAAG GATCCCCCAG ACCTTCTGTT 
TTGTCATTGC CAGAGTCGAA TGATGCTCTA AAGAAAGAGT CCAGTAAACC CAGTGGTAAA 
GTGTTGGCAT ACTCTGATGT GGATGACATT TTTGCCTCCG ACGTTCTGTT TGAGGCCACG 
TCGGTGACAA ACACACCCCC CACCGTACTG TCAACCGCGA AGACACCACC TGTAGGCAGC 
GGCGTGTTGG CTGCTAAAAA AGACAAAGAC AAAAGCACAT TCCAGTCTAT TTTTGATGAC 
GATGTGGATG ATTTGTTTCA ACCAGCCAAA CCGAGGTCGA CGGCCAAGAA AGCCAAGCAG 
TCGAAATTCT TGGAAGACGA CGATGATGAC GAGGACATAT TCGGTGTCAG CAACAGTTCC 
ACTCCGTCAT CGACCAGTAG TAAAGAAATG AAGAATGGTA GTAGTTTTTC CAAGCAGGAA 
ATTTTCCAGG ACGAAGTAAC TACTGTGCCT AAAGTTCAAA AGAAGCACAA AGAGAAGCCT 
TTAGATTTCA GCTTGTTCGA TGATGACACA GACATTTTTG CTGATCTGAC AGACACTTTC 
AAACCAAAGC AGAAACTCAA GACAAAGCCA GAGGCCAAGT CGATATTTGA CGATGACATG 
GATGACATCT TCTCATCGAG CAAAGTGAAG AAAGTTTCAA AGGCTTCAAA TAAATCTAAG 
AAAACGATAC CCGCCCAGGA CACGACTGCA GCGACAGATT CCAGCAACAT ATTTGACGAT 
CCACTAAACG CCCTTGGTGG GACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016893007.1
CDS	141..4487
Translation	MSGFPGGIANGPSQSEHMEKDARVWERPWTLEEMRQSSASWSLAADSGLFLFLQDFSHKMLSKTHEIEKQLDGLIRDTKATDSCLHSVFSDFLMLSNTQFIENRVCDEEVEEPLPKTDAVEKQPERTREQKEAELIPKMQEAVNYGLRVLESAFEHLDIKAGNSDSEDEEVTGRVEAILEPKDLYVDRPLPYLIGSQAFMEQDDVGLGDLSSDEMSVDSDRDSVIESDDGKEATHSDEDFIQEGQVHNIFKKKSSMLSFEDDDEEEEDEDSDIFGESDKDDDDHDPKNSGPISFADELAARIRGEPVHKQEGDRASEDDSDDMFKPPKMDEIDDDDDDDDFSPFGGSSRLFSGGKGLFDDDNDERDLFDDAPKPFVPEEKKPVKESVKVTATTAEPRKSAKKIPTGGVSIFPANSLFSPGNDSDSVRSKENGTPNSKVSAPPKVEKKTAVGGGGLFDEEDEDDDFFGGKSLQKSESVVKEKPKSKTTVDLFDEDDEDDGNIFGDKFKSTTPVQVKKEVVAEPVKPPEKKMPAGAISMFGPGTKSLLSEGLKKRRQSTSEESEKSEENGASPDVRKAAPKPSPKPQTRGLFSDDEDAQAFPTIPRSQSKPEPTNQSNTNKAPVFLFDDDEEEDLFASAVKSKPKASSSKTSPMQTSSAVSSSLFSDDEDQWLGATKTSETNSGGMKASASAPSGLTSTKTTPSSSLFEEEDDNELFAQTKESSTQKNPQPVALLFEDNGGDDEDDEDQGTLFNVNTNTALPTTKTLASASKSSSSQMPKSDTVEHVPTENQSLQQEKLTPHTSKSPEMPVGESGESKKKPARAVSLFGGINVLEKQTKTLIQDEEEEEDDDFPLKPSPPPFVMEEKEEKSRSGAFGLFEDEDDDEWNDSIFTPGKPTSKTTPKEEEEEEEEEAAEAEEKEEVEPAQTKSTGVLQDEELFSHSHEKDNDLDAGLFATSGRAESPPKAAAHNLFAEDDEDDLFSSPKPKVLPKIAEKPSKPADKAPLASPEPVSETEKPAPSPVKPKASSSRIGKLQASLKINPAAMLPGAVPTLPGAISVFPGMDPSSSSGVSSSSLSPSPVTTPVGAQTDSEGVTFDTPVQVTTLQSVQKSRTKGAGQRRPQTRAARQKAVQRSVDDRDNFTSTAASGPNPNLSDSESRLPERASQTSSSSTISTSSTPTVLAASPPSQHTVLKGSPRPSVLSLPESNDALKKESSKPSGKVLAYSDVDDIFASDVLFEATSVTNTPPTVLSTAKTPPVGSGVLAAKKDKDKSTFQSIFDDDVDDLFQPAKPRSTAKKAKQSKFLEDDDDDEDIFGVSNSSTPSSTSSKEMKNGSSFSKQEIFQDEVTTVPKVQKKHKEKPLDFSLFDDDTDIFADLTDTFKPKQKLKTKPEAKSIFDDDMDDIFSSSKVKKVSKASNKSKKTIPAQDTTAATDSSNIFDDPLNALGGT

Target ORF information:

RefSeq Version	XM_017037518.1
Organism	Cynoglossus semilaevis(tongue sole)
Definition	Cynoglossus semilaevis WASH complex subunit FAM21-like (LOC103387313), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017037518.1

ORF Insert Sequence:

ATGAGCGGTT TTCCGGGGGG AATAGCAAAT GGTCCCAGCC AGAGTGAACA CATGGAGAAA 
GATGCCCGGG TATGGGAGAG ACCCTGGACG TTGGAAGAAA TGCGGCAGAG TAGTGCCAGC 
TGGTCCTTGG CAGCAGATTC AGGGCTCTTC CTGTTTCTGC AAGACTTCTC TCACAAAATG 
CTGTCAAAGA CGCATGAGAT TGAAAAACAA TTGGATGGCC TCATCCGTGA CACCAAGGCT 
ACAGACAGCT GCCTGCACTC TGTGTTCAGT GACTTCCTCA TGCTCTCCAA TACACAGTTT 
ATAGAAAATA GAGTATGTGA TGAAGAAGTC GAAGAACCAC TGCCTAAAAC TGATGCTGTG 
GAGAAACAAC CGGAACGGAC CCGGGAGCAA AAGGAGGCGG AGCTGATCCC TAAGATGCAG 
GAAGCTGTAA ATTACGGTCT ACGAGTGCTG GAGTCGGCGT TTGAGCATCT GGACATCAAG 
GCAGGGAACT CGGATTCAGA GGATGAGGAG GTCACGGGCA GAGTAGAGGC CATCCTGGAA 
CCTAAGGATC TTTATGTGGA CAGACCACTT CCCTATCTGA TTGGTTCCCA GGCGTTCATG 
GAACAGGATG ATGTTGGACT TGGCGACCTC TCCAGTGATG AAATGTCAGT AGACAGTGAC 
AGAGACAGCG TGATTGAAAG TGACGACGGC AAAGAAGCCA CACATTCGGA TGAAGATTTT 
ATTCAAGAAG GCCAGGTTCA CAACATCTTC AAAAAGAAAT CATCTATGTT GAGCTTCGAA 
GATGACGACG AGGAAGAGGA AGACGAGGAT TCGGACATAT TTGGAGAATC AGACAAGGAT 
GATGACGACC ACGACCCGAA GAACTCGGGT CCAATATCAT TTGCTGATGA GCTGGCAGCT 
CGGATCAGAG GTGAACCAGT TCATAAACAG GAGGGAGATC GTGCCTCTGA AGATGACAGT 
GACGACATGT TCAAACCACC CAAGATGGAT GAGATCGACG ATGACGATGA TGACGATGAC 
TTTTCCCCGT TTGGAGGAAG TAGCCGCCTC TTTAGTGGGG GGAAGGGCCT GTTTGATGAT 
GACAACGACG AGCGGGACCT TTTCGATGAT GCACCAAAAC CTTTTGTACC AGAAGAAAAG 
AAACCTGTAA AAGAAAGTGT GAAAGTCACA GCCACAACAG CAGAACCTAG GAAATCTGCA 
AAGAAGATTC CAACAGGTGG CGTTTCAATA TTTCCAGCCA ACAGCCTCTT CAGTCCAGGG 
AATGATTCTG ATTCTGTTAG AAGCAAGGAG AACGGAACTC CGAATTCCAA GGTCAGCGCT 
CCTCCAAAAG TTGAAAAAAA GACTGCTGTT GGTGGTGGAG GACTCTTTGA TGAGGAGGAT 
GAGGACGACG ACTTCTTTGG TGGAAAAAGT CTACAAAAGT CTGAATCTGT TGTAAAGGAG 
AAACCTAAAT CCAAGACAAC CGTTGACCTC TTTGATGAAG ATGATGAGGA TGATGGCAAT 
ATATTCGGTG ATAAATTTAA GTCAACAACT CCAGTTCAGG TCAAGAAGGA GGTTGTTGCA 
GAACCAGTTA AACCGCCTGA GAAGAAGATG CCAGCTGGGG CCATCTCCAT GTTTGGACCT 
GGAACTAAAA GTCTCCTCAG CGAAGGCCTG AAAAAGCGTC GACAATCGAC CAGTGAGGAG 
TCGGAAAAAT CTGAGGAGAA CGGTGCTTCT CCAGATGTTA GGAAAGCTGC TCCAAAACCA 
TCCCCGAAAC CCCAGACCAG GGGTCTTTTC TCTGATGATG AAGATGCACA AGCTTTTCCA 
ACCATCCCAA GAAGTCAATC GAAGCCTGAA CCTACAAACC AGAGCAACAC CAACAAAGCT 
CCTGTGTTCT TGTTTGATGA CGATGAAGAA GAGGATCTCT TTGCTTCTGC GGTTAAATCT 
AAACCAAAAG CTAGTTCATC TAAAACCTCA CCCATGCAAA CCAGCAGTGC AGTCTCTAGC 
TCCCTCTTCA GTGATGATGA GGATCAGTGG TTAGGTGCTA CAAAAACATC AGAGACCAAC 
TCAGGAGGAA TGAAGGCCAG TGCTAGCGCC CCCTCTGGTC TCACTAGTAC CAAAACAACA 
CCCAGCAGCA GCCTGTTTGA GGAGGAAGAT GATAATGAAC TGTTTGCACA AACAAAAGAA 
TCAAGTACTC AGAAAAACCC TCAGCCAGTT GCACTACTGT TTGAAGATAA TGGTGGTGAT 
GATGAAGATG ACGAAGATCA AGGAACCCTT TTTAATGTCA ACACAAATAC TGCACTACCA 
ACTACTAAAA CACTAGCCTC AGCATCCAAG TCTTCCTCAT CCCAGATGCC TAAATCAGAC 
ACAGTAGAAC ATGTACCCAC AGAAAACCAG TCTTTGCAGC AGGAGAAGCT GACTCCACAT 
ACTTCAAAGT CTCCAGAGAT GCCTGTGGGA GAAAGTGGAG AAAGTAAAAA GAAGCCTGCT 
AGGGCTGTTA GTCTCTTCGG AGGCATTAAT GTTCTTGAAA AGCAGACAAA AACCCTAATA 
CAGGATGAAG AAGAAGAAGA AGATGATGAC TTCCCTTTGA AGCCCAGCCC TCCCCCATTT 
GTCATGGAGG AGAAGGAAGA AAAATCTAGA TCAGGGGCTT TTGGTCTGTT TGAAGATGAA 
GACGACGACG AGTGGAATGA TTCTATTTTC ACACCTGGTA AACCCACCAG CAAAACAACA 
CCCAAGGAAG AAGAAGAGGA GGAGGAGGAG GAGGCGGCGG AGGCGGAGGA GAAAGAGGAG 
GTGGAGCCAG CACAGACAAA GAGCACGGGT GTTCTCCAGG ACGAGGAGTT GTTCAGCCAC 
AGTCATGAGA AAGACAATGA CCTCGACGCC GGTCTCTTTG CCACTTCAGG GAGAGCTGAA 
AGCCCACCAA AGGCAGCAGC ACATAATTTG TTTGCAGAAG ACGATGAAGA TGACCTTTTC 
AGCTCCCCCA AGCCAAAAGT TCTTCCAAAA ATTGCAGAAA AGCCAAGTAA ACCTGCTGAC 
AAAGCACCGC TGGCCAGTCC AGAACCTGTC TCAGAGACTG AGAAACCTGC ACCAAGTCCT 
GTAAAACCTA AAGCTTCCTC ATCAAGGATT GGAAAACTTC AAGCAAGTCT CAAGATCAAT 
CCTGCTGCTA TGCTTCCTGG TGCTGTTCCC ACTTTGCCAG GTGCCATCAG TGTCTTCCCT 
GGCATGGATC CCAGCTCCTC CTCCGGTGTG TCAAGCTCCA GCCTGAGCCC CAGCCCTGTG 
ACAACTCCTG TAGGGGCTCA GACAGACAGT GAGGGAGTGA CCTTTGACAC CCCAGTCCAG 
GTCACGACAC TGCAGAGTGT ACAGAAAAGT CGCACCAAAG GTGCTGGTCA GCGCAGACCC 
CAGACCAGAG CTGCAAGACA GAAAGCTGTA CAAAGATCAG TCGACGACAG AGACAATTTC 
ACAAGTACAG CTGCGTCAGG TCCAAACCCC AATCTGTCTG ATTCAGAGTC ACGTCTACCA 
GAGAGAGCCA GCCAGACGAG TAGCAGTTCA ACGATTTCCA CCTCTTCCAC GCCCACAGTC 
TTGGCCGCCT CCCCACCTTC TCAGCACACT GTCCTGAAAG GATCCCCCAG ACCTTCTGTT 
TTGTCATTGC CAGAGTCGAA TGATGCTCTA AAGAAAGAGT CCAGTAAACC CAGTGGTAAA 
GTGTTGGCAT ACTCTGATGT GGATGACATT TTTGCCTCCG ACGTTCTGTT TGAGGCCACG 
TCGGTGACAA ACACACCCCC CACCGTACTG TCAACCGCGA AGACACCACC TGTAGGCAGC 
GGCGTGTTGG CTGCTAAAAA AGACAAAGAC AAAAGCACAT TCCAGTCTAT TTTTGATGAC 
GATGTGGATG ATTTGTTTCA ACCAGCCAAA CCGAGGTCGA CGGCCAAGAA AGCCAAGCAG 
TCGAAATTCT TGGAAGACGA CGATGATGAC GAGGACATAT TCGGTGTCAG CAACAGTTCC 
ACTCCGTCAT CGACCAGTAG TAAAGAAATG AAGAATGGTA GTAGTTTTTC CAAGCAGGAA 
ATTTTCCAGG ACGAAGTAAC TACTGTGCCT AAAGTTCAAA AGAAGCACAA AGAGAAGCCT 
TTAGATTTCA GCTTGTTCGA TGATGACACA GACATTTTTG CTGATCTGAC AGACACTTTC 
AAACCAAAGC AGAAACTCAA GACAAAGCCA GAGGCCAAGT CGATATTTGA CGATGACATG 
GATGACATCT TCTCATCGAG CAAAGTGAAG AAAGTTTCAA AGGCTTCAAA TAAATCTAAG 
AAAACGATAC CCGCCCAGGA CACGACTGCA GCGACAGATT CCAGCAACAT ATTTGACGAT 
CCACTAAACG CCCTTGGTGG GACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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電話

03-6811-6572

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03-6811-6573
050-3174-7589(受注専用)

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オンライン注文

LOC103387313 cDNA ORF clone, Cynoglossus semilaevis(tongue sole)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム