ube3c cDNA ORF clone, Cynoglossus semilaevis(tongue sole)

Gene
Clones
gRNAs

Summary

Gene Symbol	ube3c
Entrez Gene ID	103377534
Full Name	ubiquitin protein ligase E3C
Gene Type	protein-coding
Organism	Cynoglossus semilaevis(tongue sole)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008308374.2	XP_008306596.2	LOW QUALITY PROTEIN: ubiquitin-protein ligase E3C
XM_008308374.3	XP_008306596.2	LOW QUALITY PROTEIN: ubiquitin-protein ligase E3C

Pathways from BioSystems
Gene Ontology

The following ube3c gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ube3c cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCy52616	XM_008308374.3 Latest version!	Cynoglossus semilaevis ubiquitin protein ligase E3C (ube3c), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OCy52616	XM_008308374.2	Cynoglossus semilaevis ubiquitin protein ligase E3C (ube3c), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ube3c ( XM_008308374.2 ) cDNA ORF clone, Cynoglossus semilaevis(tongue sole) -> XP_008306596.2 Cynoglossus semilaevis ubiquitin protein ligase E3C (ube3c), mRNA.

Price & Availability↑

CloneID	OCy52616
Clone ID Related Accession (Same CDS sequence)	XM_008308374.2 , XM_008308374.3
Accession Version	XM_008308374.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-06
Organism	Cynoglossus semilaevis(tongue sole)
Product	LOW QUALITY PROTEIN: ubiquitin-protein ligase E3C
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024309.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 7, 2016 this sequence version replaced XM_008308374.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cynoglossus semilaevis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGTTTAGCT TTGAAGGAGA GTTCAAGACC AGACCCAAGG TATCACTTGG AGGAGCCAGT 
AAAAAGGAGG AGAAAGCATC TCTGCTCCAC CGCACTCAAG AAGAGAGAAG AAAAAGAGAG 
GATGAAAGGC GGCGTTTGAA GAATGCCATC ATCATTCAGT CCCACATACG TGGCTACCAA 
GACTTAAAAC AAGAGTATAC CACCCAGAGA GCCAAGTTTG ATGAGTGTGT GAACCAGACA 
CAGGCAGCTG GGTCTCAGCA GGTCTTGGAT GGTCCAGTTC TCTGCCTCCT GTCAAGACAA 
CTCATATTTT TTTACAGACA AAGTGTGGAT GCTCATAGAT TGATATGGTT GAGTCAGAAC 
CTGGTGAAAC ACAACAGTCA GTTTGTCTCA TTGTTGTTGA GTCCTCAGAA ACAGACGTGT 
ATATTTCAGA TCAAGAGGAT CTTGGGCTTC TGCTGCAGAC TCCTGCAGAA CTGCACTGAC 
AACAGTTTAA ATGTGGCAGT TCCCATGAGG ATGCTAGAAA TATATTCTTC AGAGAAAACC 
TATCTTCCTG TTATTCCAGA TCCTAACTAT GTAGCTACAT TACTTGAGCA GATTTTGCAC 
TATATGGTAC AGAAAGGATA CTACAGGTCG CTGTACATCA TGGTGAATAA CAGGCTCCCG 
GCCAGTCTGG AATACAGCGG CGCTCCAACT ATCCCCCTGG CAAGCACACT GTTGGAGCAC 
ATTCTCAAAC CTCTGCACTT TACCTACTCC TCCAGCACAA CGGGCGCAAG GCAGTTTGTA 
TTTGCAGCCT TTACCGAGGA GTTCATCTCT GCACCGTTTA CCGAGCAGAT CTTTCACTTC 
TTTATTCCGG CACTGTCCGA CCTCCGTTTC TCATTCCCCT TCGAGGCCTT CCTCAGCTCC 
CTGCAGGAGA CGGTGGGCTC CTCCTCGGCA GCACAGAGTC AGGCACCGTG GCTGTTCTAC 
TTTGTTCTCT CTGCAGGAGA GAACTGTTTG TGCTCACTTT CAGAGGAGGG TCTCCTGCTG 
TACCTGCGGG CTCTGCGGAC GCTGCTGCCG CTGCTGCCGG TGTCTGAGAG CAACAGCAGA 
CCTGAGGTGA ACAGTGACTC AGAGGATGAA GATGACGCTG GCGTCCATGC ACCACCCATG 
CAGGACGACA GTCGGATATC TGTGCAGCTG ATCACGGAGG AGTGTATCCA CAAGCTGGAC 
ACTAAGCAGC AGACAAACAC TCTGCTGCTG ACCCTGGTGT GGAGGGACTC GGCCAGCGAG 
GAGGTTTTCA CCATGATGGC GTCCATATGT CACACACTGA TGGTGCAGCA TCGCCTGATG 
GTGCCCAAAG TCAGACTCCT GTACAGTTTA GCTTTCAATG CACGCTTCCT CCGGCATCTG 
TGGCACCTGA TCACATCCAT GACGACTACG ATGATCACTG GCTCCATGGT GCCGCTGCTC 
CAGCTGATCT CCCGAGGTTC TCCCATGTCA TTTGAGGACT CCAACCGCAT CATTCCACTC 
TTCTATCTCT TCAGCTCGCT CTTCAGCCAC TCGCTCATCT CCGTGCATGA CAGCGAGTTT 
TTTGGCCATG AAATGGAAGA TCAGACACAG TCGTCCATGA TGCCCTTCAC GCTGTTAGAG 
CTGGTCACAC TGTCTCGCTG TCTGAGGGAC GCGTGTCTGG GAATCATCAA GCTGGCCTAC 
CCAGAGACCA AGGCGGAGCA CAGGGAGGAG TACATGGCTG CCTTTCGCAG CGTGGGGGTC 
AAGACCAACA CTGAGGTTCA GCTGCGCATC CAGGCCGAGC AGAAACGCTG GGTGCAGCTT 
TTCAAGGTCA TCACTAACCT GGTGAAGATG GTGAAGGCTC GCGACATCCG ACGCCCCTTC 
TGTCCAGCCG ACCACTGGCT GTCTGAGGAG GTCAACATCC GTGCTGACAG GGTCACGCAG 
CTGTACGTCC CCTCAGCCAG ACACGTGTGG AGGACCAGGA GAATGGGTCG CATCGGGCCG 
CTTCAGTCAA CGCTCGATGT GGGTTTAGAG CCTGCACAGC TGTCGGTGTC TGAGGAGAGA 
AACCTCGCCA TCCTCACAGA ACTGCCCTTC GTGGTTCCCT TTGAGGAGCG GGTCAAGATC 
TTTCAGAAGT TGATCTACGC TGACAAACGG GACGTGCAGG GGGACGGGCC GTTTCCTGAC 
GGCATCAACG TCACCATCAG ACGCAACTAC ATCTATGAAG ATGCGTACGA TAAGCTGTCC 
CCGGAGAACG AGCCTGACTT GAAGAAAAGG ATTAGGGTCC ATCTGCTCAA CGCCCACGGC 
CTGGACGAGG CCGGCATCGA TGGAGGGGGA ATCTTTCGGG AGTTCCTGAA CGAGCTTCTC 
AAGTCGGGCT TCAATCCGAA CCAGGGTTTC TTCAAGACCA CCAACGAGGG CCTGCTGTAT 
CCCAACCCCG CTGCACAGAT GTTGGTCGGA GACTCCTTCA CTCGACACTA CTACTTCTTG 
GGCCGGATCC TGGGAAAGGC GCTGTATGAA AACATGTTGG TGGAGTTGCC CTTCGCCACC 
TTCTTCCTGT CTAAGCTGCT GGGAACCAGT GCAGACGTGG ACATCCACCA CTTGGCCTCA 
CTGGACCCAG AAATGTACCG CAACCTTCTG TTCCTAAAGA GCTACGAGGG GGACGTGGAG 
GAGCTGGGCC TCAACTTCAC CGTGGTCAAC AACGACCTGG GAGAAGCTCA GGTGGTTGAA 
CTGAAGCTGG GGGGTAAAGA CATTCCCGTG ACCACGGCTA ACCGGATAGC CTACATCCAC 
CTAGTGGCAG ACTACAGGCT CAACAAACAG ATCCGACCTC ACTGCCTGGC CTTCAGACAG 
GGACTGGCCA ACGTGGTCAA CCTGGAGTGG CTGCGTATGT TCGACCAGCA GGAGATCCAG 
GTGCTGATAT CTGGAGCCCA CGTGCCAATT TGTCTGGATG ACCTGAAAAA GTTCACCAAC 
TACTCTGGTG GATACTCGCC CACTCACCCC GTCATCCAGA CCTTCTGGGA GGTGGTGGAC 
GGCTTCACGG ATGAGGAGAA ACGCAAGCTG CTCAAGTTTG TCACCAGCTG CTCCAGACCT 
CCACTGCTGG GCTTCAAGGA GCTGTACCCG GCCTTCTGCA TCCACAACGG GGGCACCGAC 
CTGGAGCGCC TGCCCACCGC CAGCACCTGC ATGAACCTGC TGAAGCTCCC GGAGTTCTGT 
GACCAGCACC TCATGAGGAA CAAGCTGCTC TACGCCATTG AGTCAGCTGC AGGGTTCGAG 
CTGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008306596.2
CDS	119..3367
Translation	MFSFEGEFKTRPKVSLGGASKKEEKASLLHRTQEERRKREDERRRLKNAIIIQSHIRGYQDLKQEYTTQRAKFDECVNQTQAAGSQQVLDGPVLCLLSRQLIFFYRQSVDAHRLIWLSQNLVKHNSQFVSLLLSPQKQTCIFQIKRILGFCCRLLQNCTDNSLNVAVPMRMLEIYSSEKTYLPVIPDPNYVATLLEQILHYMVQKGYYRSLYIMVNNRLPASLEYSGAPTIPLASTLLEHILKPLHFTYSSSTTGARQFVFAAFTEEFISAPFTEQIFHFFIPALSDLRFSFPFEAFLSSLQETVGSSSAAQSQAPWLFYFVLSAGENCLCSLSEEGLLLYLRALRTLLPLLPVSESNSRPEVNSDSEDEDDAGVHAPPMQDDSRISVQLITEECIHKLDTKQQTNTLLLTLVWRDSASEEVFTMMASICHTLMVQHRLMVPKVRLLYSLAFNARFLRHLWHLITSMTTTMITGSMVPLLQLISRGSPMSFEDSNRIIPLFYLFSSLFSHSLISVHDSEFFGHEMEDQTQSSMMPFTLLELVTLSRCLRDACLGIIKLAYPETKAEHREEYMAAFRSVGVKTNTEVQLRIQAEQKRWVQLFKVITNLVKMVKARDIRRPFCPADHWLSEEVNIRADRVTQLYVPSARHVWRTRRMGRIGPLQSTLDVGLEPAQLSVSEERNLAILTELPFVVPFEERVKIFQKLIYADKRDVQGDGPFPDGINVTIRRNYIYEDAYDKLSPENEPDLKKRIRVHLLNAHGLDEAGIDGGGIFREFLNELLKSGFNPNQGFFKTTNEGLLYPNPAAQMLVGDSFTRHYYFLGRILGKALYENMLVELPFATFFLSKLLGTSADVDIHHLASLDPEMYRNLLFLKSYEGDVEELGLNFTVVNNDLGEAQVVELKLGGKDIPVTTANRIAYIHLVADYRLNKQIRPHCLAFRQGLANVVNLEWLRMFDQQEIQVLISGAHVPICLDDLKKFTNYSGGYSPTHPVIQTFWEVVDGFTDEEKRKLLKFVTSCSRPPLLGFKELYPAFCIHNGGTDLERLPTASTCMNLLKLPEFCDQHLMRNKLLYAIESAAGFELS

Target ORF information:

RefSeq Version	XM_008308374.2
Organism	Cynoglossus semilaevis(tongue sole)
Definition	Cynoglossus semilaevis ubiquitin protein ligase E3C (ube3c), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008308374.2

ORF Insert Sequence:

ATGTTTAGCT TTGAAGGAGA GTTCAAGACC AGACCCAAGG TATCACTTGG AGGAGCCAGT 
AAAAAGGAGG AGAAAGCATC TCTGCTCCAC CGCACTCAAG AAGAGAGAAG AAAAAGAGAG 
GATGAAAGGC GGCGTTTGAA GAATGCCATC ATCATTCAGT CCCACATACG TGGCTACCAA 
GACTTAAAAC AAGAGTATAC CACCCAGAGA GCCAAGTTTG ATGAGTGTGT GAACCAGACA 
CAGGCAGCTG GGTCTCAGCA GGTCTTGGAT GGTCCAGTTC TCTGCCTCCT GTCAAGACAA 
CTCATATTTT TTTACAGACA AAGTGTGGAT GCTCATAGAT TGATATGGTT GAGTCAGAAC 
CTGGTGAAAC ACAACAGTCA GTTTGTCTCA TTGTTGTTGA GTCCTCAGAA ACAGACGTGT 
ATATTTCAGA TCAAGAGGAT CTTGGGCTTC TGCTGCAGAC TCCTGCAGAA CTGCACTGAC 
AACAGTTTAA ATGTGGCAGT TCCCATGAGG ATGCTAGAAA TATATTCTTC AGAGAAAACC 
TATCTTCCTG TTATTCCAGA TCCTAACTAT GTAGCTACAT TACTTGAGCA GATTTTGCAC 
TATATGGTAC AGAAAGGATA CTACAGGTCG CTGTACATCA TGGTGAATAA CAGGCTCCCG 
GCCAGTCTGG AATACAGCGG CGCTCCAACT ATCCCCCTGG CAAGCACACT GTTGGAGCAC 
ATTCTCAAAC CTCTGCACTT TACCTACTCC TCCAGCACAA CGGGCGCAAG GCAGTTTGTA 
TTTGCAGCCT TTACCGAGGA GTTCATCTCT GCACCGTTTA CCGAGCAGAT CTTTCACTTC 
TTTATTCCGG CACTGTCCGA CCTCCGTTTC TCATTCCCCT TCGAGGCCTT CCTCAGCTCC 
CTGCAGGAGA CGGTGGGCTC CTCCTCGGCA GCACAGAGTC AGGCACCGTG GCTGTTCTAC 
TTTGTTCTCT CTGCAGGAGA GAACTGTTTG TGCTCACTTT CAGAGGAGGG TCTCCTGCTG 
TACCTGCGGG CTCTGCGGAC GCTGCTGCCG CTGCTGCCGG TGTCTGAGAG CAACAGCAGA 
CCTGAGGTGA ACAGTGACTC AGAGGATGAA GATGACGCTG GCGTCCATGC ACCACCCATG 
CAGGACGACA GTCGGATATC TGTGCAGCTG ATCACGGAGG AGTGTATCCA CAAGCTGGAC 
ACTAAGCAGC AGACAAACAC TCTGCTGCTG ACCCTGGTGT GGAGGGACTC GGCCAGCGAG 
GAGGTTTTCA CCATGATGGC GTCCATATGT CACACACTGA TGGTGCAGCA TCGCCTGATG 
GTGCCCAAAG TCAGACTCCT GTACAGTTTA GCTTTCAATG CACGCTTCCT CCGGCATCTG 
TGGCACCTGA TCACATCCAT GACGACTACG ATGATCACTG GCTCCATGGT GCCGCTGCTC 
CAGCTGATCT CCCGAGGTTC TCCCATGTCA TTTGAGGACT CCAACCGCAT CATTCCACTC 
TTCTATCTCT TCAGCTCGCT CTTCAGCCAC TCGCTCATCT CCGTGCATGA CAGCGAGTTT 
TTTGGCCATG AAATGGAAGA TCAGACACAG TCGTCCATGA TGCCCTTCAC GCTGTTAGAG 
CTGGTCACAC TGTCTCGCTG TCTGAGGGAC GCGTGTCTGG GAATCATCAA GCTGGCCTAC 
CCAGAGACCA AGGCGGAGCA CAGGGAGGAG TACATGGCTG CCTTTCGCAG CGTGGGGGTC 
AAGACCAACA CTGAGGTTCA GCTGCGCATC CAGGCCGAGC AGAAACGCTG GGTGCAGCTT 
TTCAAGGTCA TCACTAACCT GGTGAAGATG GTGAAGGCTC GCGACATCCG ACGCCCCTTC 
TGTCCAGCCG ACCACTGGCT GTCTGAGGAG GTCAACATCC GTGCTGACAG GGTCACGCAG 
CTGTACGTCC CCTCAGCCAG ACACGTGTGG AGGACCAGGA GAATGGGTCG CATCGGGCCG 
CTTCAGTCAA CGCTCGATGT GGGTTTAGAG CCTGCACAGC TGTCGGTGTC TGAGGAGAGA 
AACCTCGCCA TCCTCACAGA ACTGCCCTTC GTGGTTCCCT TTGAGGAGCG GGTCAAGATC 
TTTCAGAAGT TGATCTACGC TGACAAACGG GACGTGCAGG GGGACGGGCC GTTTCCTGAC 
GGCATCAACG TCACCATCAG ACGCAACTAC ATCTATGAAG ATGCGTACGA TAAGCTGTCC 
CCGGAGAACG AGCCTGACTT GAAGAAAAGG ATTAGGGTCC ATCTGCTCAA CGCCCACGGC 
CTGGACGAGG CCGGCATCGA TGGAGGGGGA ATCTTTCGGG AGTTCCTGAA CGAGCTTCTC 
AAGTCGGGCT TCAATCCGAA CCAGGGTTTC TTCAAGACCA CCAACGAGGG CCTGCTGTAT 
CCCAACCCCG CTGCACAGAT GTTGGTCGGA GACTCCTTCA CTCGACACTA CTACTTCTTG 
GGCCGGATCC TGGGAAAGGC GCTGTATGAA AACATGTTGG TGGAGTTGCC CTTCGCCACC 
TTCTTCCTGT CTAAGCTGCT GGGAACCAGT GCAGACGTGG ACATCCACCA CTTGGCCTCA 
CTGGACCCAG AAATGTACCG CAACCTTCTG TTCCTAAAGA GCTACGAGGG GGACGTGGAG 
GAGCTGGGCC TCAACTTCAC CGTGGTCAAC AACGACCTGG GAGAAGCTCA GGTGGTTGAA 
CTGAAGCTGG GGGGTAAAGA CATTCCCGTG ACCACGGCTA ACCGGATAGC CTACATCCAC 
CTAGTGGCAG ACTACAGGCT CAACAAACAG ATCCGACCTC ACTGCCTGGC CTTCAGACAG 
GGACTGGCCA ACGTGGTCAA CCTGGAGTGG CTGCGTATGT TCGACCAGCA GGAGATCCAG 
GTGCTGATAT CTGGAGCCCA CGTGCCAATT TGTCTGGATG ACCTGAAAAA GTTCACCAAC 
TACTCTGGTG GATACTCGCC CACTCACCCC GTCATCCAGA CCTTCTGGGA GGTGGTGGAC 
GGCTTCACGG ATGAGGAGAA ACGCAAGCTG CTCAAGTTTG TCACCAGCTG CTCCAGACCT 
CCACTGCTGG GCTTCAAGGA GCTGTACCCG GCCTTCTGCA TCCACAACGG GGGCACCGAC 
CTGGAGCGCC TGCCCACCGC CAGCACCTGC ATGAACCTGC TGAAGCTCCC GGAGTTCTGT 
GACCAGCACC TCATGAGGAA CAAGCTGCTC TACGCCATTG AGTCAGCTGC AGGGTTCGAG 
CTGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ube3c ( XM_008308374.3 ) cDNA ORF clone, Cynoglossus semilaevis(tongue sole) -> XP_008306596.2 Cynoglossus semilaevis ubiquitin protein ligase E3C (ube3c), mRNA.

Price & Availability↑

CloneID	OCy52616
Clone ID Related Accession (Same CDS sequence)	XM_008308374.2 , XM_008308374.3
Accession Version	XM_008308374.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-15
Organism	Cynoglossus semilaevis(tongue sole)
Product	LOW QUALITY PROTEIN: ubiquitin-protein ligase E3C
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024309.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 16, 2018 this sequence version replaced XM_008308374.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cynoglossus semilaevis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGTTTAGCT TTGAAGGAGA GTTCAAGACC AGACCCAAGG TATCACTTGG AGGAGCCAGT 
AAAAAGGAGG AGAAAGCATC TCTGCTCCAC CGCACTCAAG AAGAGAGAAG AAAAAGAGAG 
GATGAAAGGC GGCGTTTGAA GAATGCCATC ATCATTCAGT CCCACATACG TGGCTACCAA 
GACTTAAAAC AAGAGTATAC CACCCAGAGA GCCAAGTTTG ATGAGTGTGT GAACCAGACA 
CAGGCAGCTG GGTCTCAGCA GGTCTTGGAT GGTCCAGTTC TCTGCCTCCT GTCAAGACAA 
CTCATATTTT TTTACAGACA AAGTGTGGAT GCTCATAGAT TGATATGGTT GAGTCAGAAC 
CTGGTGAAAC ACAACAGTCA GTTTGTCTCA TTGTTGTTGA GTCCTCAGAA ACAGACGTGT 
ATATTTCAGA TCAAGAGGAT CTTGGGCTTC TGCTGCAGAC TCCTGCAGAA CTGCACTGAC 
AACAGTTTAA ATGTGGCAGT TCCCATGAGG ATGCTAGAAA TATATTCTTC AGAGAAAACC 
TATCTTCCTG TTATTCCAGA TCCTAACTAT GTAGCTACAT TACTTGAGCA GATTTTGCAC 
TATATGGTAC AGAAAGGATA CTACAGGTCG CTGTACATCA TGGTGAATAA CAGGCTCCCG 
GCCAGTCTGG AATACAGCGG CGCTCCAACT ATCCCCCTGG CAAGCACACT GTTGGAGCAC 
ATTCTCAAAC CTCTGCACTT TACCTACTCC TCCAGCACAA CGGGCGCAAG GCAGTTTGTA 
TTTGCAGCCT TTACCGAGGA GTTCATCTCT GCACCGTTTA CCGAGCAGAT CTTTCACTTC 
TTTATTCCGG CACTGTCCGA CCTCCGTTTC TCATTCCCCT TCGAGGCCTT CCTCAGCTCC 
CTGCAGGAGA CGGTGGGCTC CTCCTCGGCA GCACAGAGTC AGGCACCGTG GCTGTTCTAC 
TTTGTTCTCT CTGCAGGAGA GAACTGTTTG TGCTCACTTT CAGAGGAGGG TCTCCTGCTG 
TACCTGCGGG CTCTGCGGAC GCTGCTGCCG CTGCTGCCGG TGTCTGAGAG CAACAGCAGA 
CCTGAGGTGA ACAGTGACTC AGAGGATGAA GATGACGCTG GCGTCCATGC ACCACCCATG 
CAGGACGACA GTCGGATATC TGTGCAGCTG ATCACGGAGG AGTGTATCCA CAAGCTGGAC 
ACTAAGCAGC AGACAAACAC TCTGCTGCTG ACCCTGGTGT GGAGGGACTC GGCCAGCGAG 
GAGGTTTTCA CCATGATGGC GTCCATATGT CACACACTGA TGGTGCAGCA TCGCCTGATG 
GTGCCCAAAG TCAGACTCCT GTACAGTTTA GCTTTCAATG CACGCTTCCT CCGGCATCTG 
TGGCACCTGA TCACATCCAT GACGACTACG ATGATCACTG GCTCCATGGT GCCGCTGCTC 
CAGCTGATCT CCCGAGGTTC TCCCATGTCA TTTGAGGACT CCAACCGCAT CATTCCACTC 
TTCTATCTCT TCAGCTCGCT CTTCAGCCAC TCGCTCATCT CCGTGCATGA CAGCGAGTTT 
TTTGGCCATG AAATGGAAGA TCAGACACAG TCGTCCATGA TGCCCTTCAC GCTGTTAGAG 
CTGGTCACAC TGTCTCGCTG TCTGAGGGAC GCGTGTCTGG GAATCATCAA GCTGGCCTAC 
CCAGAGACCA AGGCGGAGCA CAGGGAGGAG TACATGGCTG CCTTTCGCAG CGTGGGGGTC 
AAGACCAACA CTGAGGTTCA GCTGCGCATC CAGGCCGAGC AGAAACGCTG GGTGCAGCTT 
TTCAAGGTCA TCACTAACCT GGTGAAGATG GTGAAGGCTC GCGACATCCG ACGCCCCTTC 
TGTCCAGCCG ACCACTGGCT GTCTGAGGAG GTCAACATCC GTGCTGACAG GGTCACGCAG 
CTGTACGTCC CCTCAGCCAG ACACGTGTGG AGGACCAGGA GAATGGGTCG CATCGGGCCG 
CTTCAGTCAA CGCTCGATGT GGGTTTAGAG CCTGCACAGC TGTCGGTGTC TGAGGAGAGA 
AACCTCGCCA TCCTCACAGA ACTGCCCTTC GTGGTTCCCT TTGAGGAGCG GGTCAAGATC 
TTTCAGAAGT TGATCTACGC TGACAAACGG GACGTGCAGG GGGACGGGCC GTTTCCTGAC 
GGCATCAACG TCACCATCAG ACGCAACTAC ATCTATGAAG ATGCGTACGA TAAGCTGTCC 
CCGGAGAACG AGCCTGACTT GAAGAAAAGG ATTAGGGTCC ATCTGCTCAA CGCCCACGGC 
CTGGACGAGG CCGGCATCGA TGGAGGGGGA ATCTTTCGGG AGTTCCTGAA CGAGCTTCTC 
AAGTCGGGCT TCAATCCGAA CCAGGGTTTC TTCAAGACCA CCAACGAGGG CCTGCTGTAT 
CCCAACCCCG CTGCACAGAT GTTGGTCGGA GACTCCTTCA CTCGACACTA CTACTTCTTG 
GGCCGGATCC TGGGAAAGGC GCTGTATGAA AACATGTTGG TGGAGTTGCC CTTCGCCACC 
TTCTTCCTGT CTAAGCTGCT GGGAACCAGT GCAGACGTGG ACATCCACCA CTTGGCCTCA 
CTGGACCCAG AAATGTACCG CAACCTTCTG TTCCTAAAGA GCTACGAGGG GGACGTGGAG 
GAGCTGGGCC TCAACTTCAC CGTGGTCAAC AACGACCTGG GAGAAGCTCA GGTGGTTGAA 
CTGAAGCTGG GGGGTAAAGA CATTCCCGTG ACCACGGCTA ACCGGATAGC CTACATCCAC 
CTAGTGGCAG ACTACAGGCT CAACAAACAG ATCCGACCTC ACTGCCTGGC CTTCAGACAG 
GGACTGGCCA ACGTGGTCAA CCTGGAGTGG CTGCGTATGT TCGACCAGCA GGAGATCCAG 
GTGCTGATAT CTGGAGCCCA CGTGCCAATT TGTCTGGATG ACCTGAAAAA GTTCACCAAC 
TACTCTGGTG GATACTCGCC CACTCACCCC GTCATCCAGA CCTTCTGGGA GGTGGTGGAC 
GGCTTCACGG ATGAGGAGAA ACGCAAGCTG CTCAAGTTTG TCACCAGCTG CTCCAGACCT 
CCACTGCTGG GCTTCAAGGA GCTGTACCCG GCCTTCTGCA TCCACAACGG GGGCACCGAC 
CTGGAGCGCC TGCCCACCGC CAGCACCTGC ATGAACCTGC TGAAGCTCCC GGAGTTCTGT 
GACCAGCACC TCATGAGGAA CAAGCTGCTC TACGCCATTG AGTCAGCTGC AGGGTTCGAG 
CTGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008306596.2
CDS	125..3373
Translation	MFSFEGEFKTRPKVSLGGASKKEEKASLLHRTQEERRKREDERRRLKNAIIIQSHIRGYQDLKQEYTTQRAKFDECVNQTQAAGSQQVLDGPVLCLLSRQLIFFYRQSVDAHRLIWLSQNLVKHNSQFVSLLLSPQKQTCIFQIKRILGFCCRLLQNCTDNSLNVAVPMRMLEIYSSEKTYLPVIPDPNYVATLLEQILHYMVQKGYYRSLYIMVNNRLPASLEYSGAPTIPLASTLLEHILKPLHFTYSSSTTGARQFVFAAFTEEFISAPFTEQIFHFFIPALSDLRFSFPFEAFLSSLQETVGSSSAAQSQAPWLFYFVLSAGENCLCSLSEEGLLLYLRALRTLLPLLPVSESNSRPEVNSDSEDEDDAGVHAPPMQDDSRISVQLITEECIHKLDTKQQTNTLLLTLVWRDSASEEVFTMMASICHTLMVQHRLMVPKVRLLYSLAFNARFLRHLWHLITSMTTTMITGSMVPLLQLISRGSPMSFEDSNRIIPLFYLFSSLFSHSLISVHDSEFFGHEMEDQTQSSMMPFTLLELVTLSRCLRDACLGIIKLAYPETKAEHREEYMAAFRSVGVKTNTEVQLRIQAEQKRWVQLFKVITNLVKMVKARDIRRPFCPADHWLSEEVNIRADRVTQLYVPSARHVWRTRRMGRIGPLQSTLDVGLEPAQLSVSEERNLAILTELPFVVPFEERVKIFQKLIYADKRDVQGDGPFPDGINVTIRRNYIYEDAYDKLSPENEPDLKKRIRVHLLNAHGLDEAGIDGGGIFREFLNELLKSGFNPNQGFFKTTNEGLLYPNPAAQMLVGDSFTRHYYFLGRILGKALYENMLVELPFATFFLSKLLGTSADVDIHHLASLDPEMYRNLLFLKSYEGDVEELGLNFTVVNNDLGEAQVVELKLGGKDIPVTTANRIAYIHLVADYRLNKQIRPHCLAFRQGLANVVNLEWLRMFDQQEIQVLISGAHVPICLDDLKKFTNYSGGYSPTHPVIQTFWEVVDGFTDEEKRKLLKFVTSCSRPPLLGFKELYPAFCIHNGGTDLERLPTASTCMNLLKLPEFCDQHLMRNKLLYAIESAAGFELS

Target ORF information:

RefSeq Version	XM_008308374.3
Organism	Cynoglossus semilaevis(tongue sole)
Definition	Cynoglossus semilaevis ubiquitin protein ligase E3C (ube3c), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008308374.3

ORF Insert Sequence:

ATGTTTAGCT TTGAAGGAGA GTTCAAGACC AGACCCAAGG TATCACTTGG AGGAGCCAGT 
AAAAAGGAGG AGAAAGCATC TCTGCTCCAC CGCACTCAAG AAGAGAGAAG AAAAAGAGAG 
GATGAAAGGC GGCGTTTGAA GAATGCCATC ATCATTCAGT CCCACATACG TGGCTACCAA 
GACTTAAAAC AAGAGTATAC CACCCAGAGA GCCAAGTTTG ATGAGTGTGT GAACCAGACA 
CAGGCAGCTG GGTCTCAGCA GGTCTTGGAT GGTCCAGTTC TCTGCCTCCT GTCAAGACAA 
CTCATATTTT TTTACAGACA AAGTGTGGAT GCTCATAGAT TGATATGGTT GAGTCAGAAC 
CTGGTGAAAC ACAACAGTCA GTTTGTCTCA TTGTTGTTGA GTCCTCAGAA ACAGACGTGT 
ATATTTCAGA TCAAGAGGAT CTTGGGCTTC TGCTGCAGAC TCCTGCAGAA CTGCACTGAC 
AACAGTTTAA ATGTGGCAGT TCCCATGAGG ATGCTAGAAA TATATTCTTC AGAGAAAACC 
TATCTTCCTG TTATTCCAGA TCCTAACTAT GTAGCTACAT TACTTGAGCA GATTTTGCAC 
TATATGGTAC AGAAAGGATA CTACAGGTCG CTGTACATCA TGGTGAATAA CAGGCTCCCG 
GCCAGTCTGG AATACAGCGG CGCTCCAACT ATCCCCCTGG CAAGCACACT GTTGGAGCAC 
ATTCTCAAAC CTCTGCACTT TACCTACTCC TCCAGCACAA CGGGCGCAAG GCAGTTTGTA 
TTTGCAGCCT TTACCGAGGA GTTCATCTCT GCACCGTTTA CCGAGCAGAT CTTTCACTTC 
TTTATTCCGG CACTGTCCGA CCTCCGTTTC TCATTCCCCT TCGAGGCCTT CCTCAGCTCC 
CTGCAGGAGA CGGTGGGCTC CTCCTCGGCA GCACAGAGTC AGGCACCGTG GCTGTTCTAC 
TTTGTTCTCT CTGCAGGAGA GAACTGTTTG TGCTCACTTT CAGAGGAGGG TCTCCTGCTG 
TACCTGCGGG CTCTGCGGAC GCTGCTGCCG CTGCTGCCGG TGTCTGAGAG CAACAGCAGA 
CCTGAGGTGA ACAGTGACTC AGAGGATGAA GATGACGCTG GCGTCCATGC ACCACCCATG 
CAGGACGACA GTCGGATATC TGTGCAGCTG ATCACGGAGG AGTGTATCCA CAAGCTGGAC 
ACTAAGCAGC AGACAAACAC TCTGCTGCTG ACCCTGGTGT GGAGGGACTC GGCCAGCGAG 
GAGGTTTTCA CCATGATGGC GTCCATATGT CACACACTGA TGGTGCAGCA TCGCCTGATG 
GTGCCCAAAG TCAGACTCCT GTACAGTTTA GCTTTCAATG CACGCTTCCT CCGGCATCTG 
TGGCACCTGA TCACATCCAT GACGACTACG ATGATCACTG GCTCCATGGT GCCGCTGCTC 
CAGCTGATCT CCCGAGGTTC TCCCATGTCA TTTGAGGACT CCAACCGCAT CATTCCACTC 
TTCTATCTCT TCAGCTCGCT CTTCAGCCAC TCGCTCATCT CCGTGCATGA CAGCGAGTTT 
TTTGGCCATG AAATGGAAGA TCAGACACAG TCGTCCATGA TGCCCTTCAC GCTGTTAGAG 
CTGGTCACAC TGTCTCGCTG TCTGAGGGAC GCGTGTCTGG GAATCATCAA GCTGGCCTAC 
CCAGAGACCA AGGCGGAGCA CAGGGAGGAG TACATGGCTG CCTTTCGCAG CGTGGGGGTC 
AAGACCAACA CTGAGGTTCA GCTGCGCATC CAGGCCGAGC AGAAACGCTG GGTGCAGCTT 
TTCAAGGTCA TCACTAACCT GGTGAAGATG GTGAAGGCTC GCGACATCCG ACGCCCCTTC 
TGTCCAGCCG ACCACTGGCT GTCTGAGGAG GTCAACATCC GTGCTGACAG GGTCACGCAG 
CTGTACGTCC CCTCAGCCAG ACACGTGTGG AGGACCAGGA GAATGGGTCG CATCGGGCCG 
CTTCAGTCAA CGCTCGATGT GGGTTTAGAG CCTGCACAGC TGTCGGTGTC TGAGGAGAGA 
AACCTCGCCA TCCTCACAGA ACTGCCCTTC GTGGTTCCCT TTGAGGAGCG GGTCAAGATC 
TTTCAGAAGT TGATCTACGC TGACAAACGG GACGTGCAGG GGGACGGGCC GTTTCCTGAC 
GGCATCAACG TCACCATCAG ACGCAACTAC ATCTATGAAG ATGCGTACGA TAAGCTGTCC 
CCGGAGAACG AGCCTGACTT GAAGAAAAGG ATTAGGGTCC ATCTGCTCAA CGCCCACGGC 
CTGGACGAGG CCGGCATCGA TGGAGGGGGA ATCTTTCGGG AGTTCCTGAA CGAGCTTCTC 
AAGTCGGGCT TCAATCCGAA CCAGGGTTTC TTCAAGACCA CCAACGAGGG CCTGCTGTAT 
CCCAACCCCG CTGCACAGAT GTTGGTCGGA GACTCCTTCA CTCGACACTA CTACTTCTTG 
GGCCGGATCC TGGGAAAGGC GCTGTATGAA AACATGTTGG TGGAGTTGCC CTTCGCCACC 
TTCTTCCTGT CTAAGCTGCT GGGAACCAGT GCAGACGTGG ACATCCACCA CTTGGCCTCA 
CTGGACCCAG AAATGTACCG CAACCTTCTG TTCCTAAAGA GCTACGAGGG GGACGTGGAG 
GAGCTGGGCC TCAACTTCAC CGTGGTCAAC AACGACCTGG GAGAAGCTCA GGTGGTTGAA 
CTGAAGCTGG GGGGTAAAGA CATTCCCGTG ACCACGGCTA ACCGGATAGC CTACATCCAC 
CTAGTGGCAG ACTACAGGCT CAACAAACAG ATCCGACCTC ACTGCCTGGC CTTCAGACAG 
GGACTGGCCA ACGTGGTCAA CCTGGAGTGG CTGCGTATGT TCGACCAGCA GGAGATCCAG 
GTGCTGATAT CTGGAGCCCA CGTGCCAATT TGTCTGGATG ACCTGAAAAA GTTCACCAAC 
TACTCTGGTG GATACTCGCC CACTCACCCC GTCATCCAGA CCTTCTGGGA GGTGGTGGAC 
GGCTTCACGG ATGAGGAGAA ACGCAAGCTG CTCAAGTTTG TCACCAGCTG CTCCAGACCT 
CCACTGCTGG GCTTCAAGGA GCTGTACCCG GCCTTCTGCA TCCACAACGG GGGCACCGAC 
CTGGAGCGCC TGCCCACCGC CAGCACCTGC ATGAACCTGC TGAAGCTCCC GGAGTTCTGT 
GACCAGCACC TCATGAGGAA CAAGCTGCTC TACGCCATTG AGTCAGCTGC AGGGTTCGAG 
CTGAGCTGA