DGKI cDNA ORF clone, Cuculus canorus(common cuckoo)

Gene
Clones
gRNAs

Summary

Gene Symbol	DGKI
Entrez Gene ID	104058949
Full Name	diacylglycerol kinase iota
Gene Type	protein-coding
Organism	Cuculus canorus(common cuckoo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009560541.1	XP_009558836.1	diacylglycerol kinase iota

Pathways from BioSystems
Gene Ontology

The following DGKI gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DGKI cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCu44331	XM_009560541.1 Latest version!	Cuculus canorus diacylglycerol kinase, iota (DGKI), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DGKI ( XM_009560541.1 ) cDNA ORF clone, Cuculus canorus(common cuckoo) -> XP_009558836.1 Cuculus canorus diacylglycerol kinase, iota (DGKI), mRNA.

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CloneID	OCu44331
Clone ID Related Accession (Same CDS sequence)	XM_009560541.1
Accession Version	XM_009560541.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-13
Organism	Cuculus canorus(common cuckoo)
Product	diacylglycerol kinase iota
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009244915.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cuculus canorus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGT TGGAGAAGAT TAATTTTCGC TGCAAACCAA CTTTCCGAGA AGGAGGCTCC 
AGATCTCCAA GAGAAAACTT TGTCAGACAC CACTGGGTGC ACAGGCGGAG GCAGGAGGGG 
AAATGTAAGC AGTGTGGAAA GGGCTTTCAG CAGAAATTCT CCTTCCATAG TAAAGAGATT 
GTGGCCATCA GTTGTTCCTG GTGCAAGCTG GCGTTTCATA ACAAAGTCAC CTGTTTCATG 
CTTCATCACA TTGAGGAGCC ATGTTCCTTG GGGGCTCACG CTGCTGTCAT TGTGCCTCCC 
ACCTGGATCA TTAAGGTGAA GAAACCTCAG GTGGTCTCCA TACCAGGAGA CGAGCCTGAT 
TGGGCAGGAG ACTGGAGCCC CGAAGGGGAA ACGGGCGTCT CCTACCCCTA TTGGGAGGGT 
TGGCAGAACT CATTAAAGAC TTCAACACGG CGAAAGAAGA GAACATCTTT TAAAAGAAAA 
GCCAGCAAGA GAGGCAATGA GGATAACAAA GGTCGGCCAT TTGTGATAAA GCCTATCTCC 
TCTCCACTCA TGAAGCCGCT GCTGGTTTTT GTCAATCCAA AAAGTGGAGG GAATCAGGGC 
ACCAAGGTTC TCCAAATGTT TATGTGGTAT TTGAATCCAC GCCAGGTCTT TGATCTCTCT 
CAGGAAGGGC CAAGAGATGC GCTGGAGCTG TACAGAAAAG TGCCAAACCT GCGGATTCTG 
GCCTGTGGTG GGGATGGGAC GGTGGGTTGG ATCCTCTCCA TTCTTGATGA GCTGCAACTC 
AGCCCCCCGC CTCCTGTGGC TGTCCTTCCA CTTGGCACTG GAAATGACCT TGCCCGCACC 
CTCAACTGGG GCGGGGGTTA CACAGATGAG CCCGTGTCCA AGATCCTGTG CCATGTAGAA 
GATGGGACCA TTGTCCAGCT GGACCGCTGG AATCTCCAGG TTGAGCGCAA CCCTGACTTG 
CCACAGGATG AGCTGGAGGA TGGGGCACGT AAGCTTCCTC TCAATGTCTT CAATAATTAC 
TTTAGCCTTG GATTTGATGC ACATGTTACA CTGGAGTTCC ATGAATCTAG AGAAGCAAAT 
CCAGAGAAAT TCAACAGCCG ATTTAGAAAT AAAATGTTTT ATGCGGGGGC AGCCTTTACA 
GACTTTCTGC AAAGAAGCTC AAGAGATTTA TCCAAGCATG TTAAAGTTGT GTGTGATGGT 
ACAGACCTGA CTCCAAAGAT CCAGGAGCTG AAGTTCCAGT GTATAGTGTT TTTAAACATA 
CCCAGATATT GTGCTGGCAC AATGCCCTGG GGAAACCCTG GAGATCACAG AGACTTTGAA 
CCTCAGCGCC ATGATGATGG CTACATTGAA GTCATTGGGT TCACCATGGC CTCACTGGCT 
GCTCTTCAGG TGGGTGGTCA TGGAGAGAGG TTGCATCAGT GCCGTGAAGT GACTCTTTTA 
ACATACAAGT CTATTCCCAT GCAAGTGGAT GGTGAACCAT GTCGATTGGC TCCCTCACTC 
ATTCGAATCT CCTTAAGGAA CCAAGCCAAC ATGGTGCAGA AGAGCAAGAG GAGAACATCT 
ATGCCTTTGC TGAATGATCC TCATTCCATC CCAGATCGCC TGCGTATCAG AGTGAACAGG 
ATTAATTTAC AAGAATATGA GGGGCTACAT TACGACAAGG AAAAACTCCG GGAAGCTTCC 
ATTCCCCTAG GAATTATAGT TGTACGAGGA GATTGTGACC TGGAGACCTG TCGTATGTAC 
ATTGACCGTC TGCAAGAGGA CCTACAGTCG GTAACTTCTA CTACACAGAG AGTTCATTAC 
CAGGACCAAG AAAGCTCTTT TCCCAGAGTA CTTTCTGTTC AGAGACTATC TCCTAGATGG 
TGCTTTCTAG ATGCAACTTC TGCTGATCGT TTTTACCGCA TTGACAGATC TCAGGAACAT 
TTGCACTTTG TGACGGAAAT TTCGCAGGAC GAGATTTTTA TTCTGGACCC AGAGGTGGTA 
ATGTCCCAGC AGGTGGGGAC TCCACCAGGN NNNNCTGATC TGGTAGTGGA GCAGGCCTCC 
GGAATATCAG ATAGGTGGAA CCCTGCAGCT CGAAAGAGGA TGCTGAGTGA CAGTGGCCTT 
GGCAAGATTT CCCCACACTA CGAGGTACCT GACAAACAAA AGGACGCCAG CCAGACACAT 
ATGCTGCAGT CACCAGCCTC TTCAGAAGAT CAAGCGCTTT TACAGGCAGT CATAGCTGGT 
GATCTGCTGA AGTTCATAGA ATGCTGTAAA AAGGGAGCCA ATTTGTTAAT CCAAGGACCT 
GATCACTGCT CCTTGTTGCA CCATGCTGCC AAGACTGGGC ATGGTGAGAT TGTGAAATAC 
ATCCTGGAGC ATGGTCCATC TGAATTACTG GACATGACAG ATAGTGAAAC GGGTGAGACA 
GCATTACATA AGGCAGCCTG CCAGCGCCAT CGTGCCGTCT GCCAGCTCCT GGTGGATGCA 
GGAGCCTCTC TGAGAAAGAC AGACTCCAAG GGGAAGACTC CTCGTGACAG GGCTCAGCAA 
GCTGGTGACC CGGATCTGGC CTCCTACCTG GAGAGTCGAC AAAATTACCA AATGGTTTCC 
CACGAGGATC TGGAGACAGC AGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009558836.1
CDS	1..2607
Translation	MEQLEKINFRCKPTFREGGSRSPRENFVRHHWVHRRRQEGKCKQCGKGFQQKFSFHSKEIVAISCSWCKLAFHNKVTCFMLHHIEEPCSLGAHAAVIVPPTWIIKVKKPQVVSIPGDEPDWAGDWSPEGETGVSYPYWEGWQNSLKTSTRRKKRTSFKRKASKRGNEDNKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPRDALELYRKVPNLRILACGGDGTVGWILSILDELQLSPPPPVAVLPLGTGNDLARTLNWGGGYTDEPVSKILCHVEDGTIVQLDRWNLQVERNPDLPQDELEDGARKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFTDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHRDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVTLLTYKSIPMQVDGEPCRLAPSLIRISLRNQANMVQKSKRRTSMPLLNDPHSIPDRLRIRVNRINLQEYEGLHYDKEKLREASIPLGIIVVRGDCDLETCRMYIDRLQEDLQSVTSTTQRVHYQDQESSFPRVLSVQRLSPRWCFLDATSADRFYRIDRSQEHLHFVTEISQDEIFILDPEVVMSQQVGTPPnullnullnullDLVVEQASGISDRWNPAARKRMLSDSGLGKISPHYEVPDKQKDASQTHMLQSPASSEDQALLQAVIAGDLLKFIECCKKGANLLIQGPDHCSLLHHAAKTGHGEIVKYILEHGPSELLDMTDSETGETALHKAACQRHRAVCQLLVDAGASLRKTDSKGKTPRDRAQQAGDPDLASYLESRQNYQMVSHEDLETAV

Target ORF information:

RefSeq Version	XM_009560541.1
Organism	Cuculus canorus(common cuckoo)
Definition	Cuculus canorus diacylglycerol kinase, iota (DGKI), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009560541.1

ORF Insert Sequence:

ATGGAGCAGT TGGAGAAGAT TAATTTTCGC TGCAAACCAA CTTTCCGAGA AGGAGGCTCC 
AGATCTCCAA GAGAAAACTT TGTCAGACAC CACTGGGTGC ACAGGCGGAG GCAGGAGGGG 
AAATGTAAGC AGTGTGGAAA GGGCTTTCAG CAGAAATTCT CCTTCCATAG TAAAGAGATT 
GTGGCCATCA GTTGTTCCTG GTGCAAGCTG GCGTTTCATA ACAAAGTCAC CTGTTTCATG 
CTTCATCACA TTGAGGAGCC ATGTTCCTTG GGGGCTCACG CTGCTGTCAT TGTGCCTCCC 
ACCTGGATCA TTAAGGTGAA GAAACCTCAG GTGGTCTCCA TACCAGGAGA CGAGCCTGAT 
TGGGCAGGAG ACTGGAGCCC CGAAGGGGAA ACGGGCGTCT CCTACCCCTA TTGGGAGGGT 
TGGCAGAACT CATTAAAGAC TTCAACACGG CGAAAGAAGA GAACATCTTT TAAAAGAAAA 
GCCAGCAAGA GAGGCAATGA GGATAACAAA GGTCGGCCAT TTGTGATAAA GCCTATCTCC 
TCTCCACTCA TGAAGCCGCT GCTGGTTTTT GTCAATCCAA AAAGTGGAGG GAATCAGGGC 
ACCAAGGTTC TCCAAATGTT TATGTGGTAT TTGAATCCAC GCCAGGTCTT TGATCTCTCT 
CAGGAAGGGC CAAGAGATGC GCTGGAGCTG TACAGAAAAG TGCCAAACCT GCGGATTCTG 
GCCTGTGGTG GGGATGGGAC GGTGGGTTGG ATCCTCTCCA TTCTTGATGA GCTGCAACTC 
AGCCCCCCGC CTCCTGTGGC TGTCCTTCCA CTTGGCACTG GAAATGACCT TGCCCGCACC 
CTCAACTGGG GCGGGGGTTA CACAGATGAG CCCGTGTCCA AGATCCTGTG CCATGTAGAA 
GATGGGACCA TTGTCCAGCT GGACCGCTGG AATCTCCAGG TTGAGCGCAA CCCTGACTTG 
CCACAGGATG AGCTGGAGGA TGGGGCACGT AAGCTTCCTC TCAATGTCTT CAATAATTAC 
TTTAGCCTTG GATTTGATGC ACATGTTACA CTGGAGTTCC ATGAATCTAG AGAAGCAAAT 
CCAGAGAAAT TCAACAGCCG ATTTAGAAAT AAAATGTTTT ATGCGGGGGC AGCCTTTACA 
GACTTTCTGC AAAGAAGCTC AAGAGATTTA TCCAAGCATG TTAAAGTTGT GTGTGATGGT 
ACAGACCTGA CTCCAAAGAT CCAGGAGCTG AAGTTCCAGT GTATAGTGTT TTTAAACATA 
CCCAGATATT GTGCTGGCAC AATGCCCTGG GGAAACCCTG GAGATCACAG AGACTTTGAA 
CCTCAGCGCC ATGATGATGG CTACATTGAA GTCATTGGGT TCACCATGGC CTCACTGGCT 
GCTCTTCAGG TGGGTGGTCA TGGAGAGAGG TTGCATCAGT GCCGTGAAGT GACTCTTTTA 
ACATACAAGT CTATTCCCAT GCAAGTGGAT GGTGAACCAT GTCGATTGGC TCCCTCACTC 
ATTCGAATCT CCTTAAGGAA CCAAGCCAAC ATGGTGCAGA AGAGCAAGAG GAGAACATCT 
ATGCCTTTGC TGAATGATCC TCATTCCATC CCAGATCGCC TGCGTATCAG AGTGAACAGG 
ATTAATTTAC AAGAATATGA GGGGCTACAT TACGACAAGG AAAAACTCCG GGAAGCTTCC 
ATTCCCCTAG GAATTATAGT TGTACGAGGA GATTGTGACC TGGAGACCTG TCGTATGTAC 
ATTGACCGTC TGCAAGAGGA CCTACAGTCG GTAACTTCTA CTACACAGAG AGTTCATTAC 
CAGGACCAAG AAAGCTCTTT TCCCAGAGTA CTTTCTGTTC AGAGACTATC TCCTAGATGG 
TGCTTTCTAG ATGCAACTTC TGCTGATCGT TTTTACCGCA TTGACAGATC TCAGGAACAT 
TTGCACTTTG TGACGGAAAT TTCGCAGGAC GAGATTTTTA TTCTGGACCC AGAGGTGGTA 
ATGTCCCAGC AGGTGGGGAC TCCACCAGGN NNNNCTGATC TGGTAGTGGA GCAGGCCTCC 
GGAATATCAG ATAGGTGGAA CCCTGCAGCT CGAAAGAGGA TGCTGAGTGA CAGTGGCCTT 
GGCAAGATTT CCCCACACTA CGAGGTACCT GACAAACAAA AGGACGCCAG CCAGACACAT 
ATGCTGCAGT CACCAGCCTC TTCAGAAGAT CAAGCGCTTT TACAGGCAGT CATAGCTGGT 
GATCTGCTGA AGTTCATAGA ATGCTGTAAA AAGGGAGCCA ATTTGTTAAT CCAAGGACCT 
GATCACTGCT CCTTGTTGCA CCATGCTGCC AAGACTGGGC ATGGTGAGAT TGTGAAATAC 
ATCCTGGAGC ATGGTCCATC TGAATTACTG GACATGACAG ATAGTGAAAC GGGTGAGACA 
GCATTACATA AGGCAGCCTG CCAGCGCCAT CGTGCCGTCT GCCAGCTCCT GGTGGATGCA 
GGAGCCTCTC TGAGAAAGAC AGACTCCAAG GGGAAGACTC CTCGTGACAG GGCTCAGCAA 
GCTGGTGACC CGGATCTGGC CTCCTACCTG GAGAGTCGAC AAAATTACCA AATGGTTTCC 
CACGAGGATC TGGAGACAGC AGTCTGA