PCM1 gene cDNA ORF clone, Columba livia(rock pigeon)

mRNA	Protein	Name
XM_013366633.1	XP_013222087.1	pericentriolar material 1 protein isoform X2
XM_021285854.1	XP_021141529.1	pericentriolar material 1 protein isoform X1
XM_021285857.1	XP_021141532.1	pericentriolar material 1 protein isoform X1
XM_021285859.1	XP_021141534.1	pericentriolar material 1 protein isoform X3
XM_021285863.1	XP_021141538.1	pericentriolar material 1 protein isoform X7
XM_021285869.1	XP_021141544.1	pericentriolar material 1 protein isoform X13
XM_021285870.1	XP_021141545.1	pericentriolar material 1 protein isoform X14
XM_013366632.1	XP_013222086.1	pericentriolar material 1 protein isoform X1
XM_021285856.1	XP_021141531.1	pericentriolar material 1 protein isoform X1
XM_021285858.1	XP_021141533.1	pericentriolar material 1 protein isoform X2
XM_021285860.1	XP_021141535.1	pericentriolar material 1 protein isoform X4
XM_021285862.1	XP_021141537.1	pericentriolar material 1 protein isoform X6
XM_021285867.1	XP_021141542.1	pericentriolar material 1 protein isoform X11
XM_021285868.1	XP_021141543.1	pericentriolar material 1 protein isoform X12
XM_021285871.1	XP_021141546.1	pericentriolar material 1 protein isoform X15
XM_021285855.1	XP_021141530.1	pericentriolar material 1 protein isoform X1
XM_021285865.1	XP_021141540.1	pericentriolar material 1 protein isoform X9
XM_021285866.1	XP_021141541.1	pericentriolar material 1 protein isoform X10
XM_021285861.1	XP_021141536.1	pericentriolar material 1 protein isoform X5
XM_021285864.1	XP_021141539.1	pericentriolar material 1 protein isoform X8

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCo260086	XM_013366633.1 Latest version!	Columba livia pericentriolar material 1 (PCM1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCo293171	XM_021285868.1 Latest version!	Columba livia pericentriolar material 1 (PCM1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCo293172	XM_021285869.1 Latest version!	Columba livia pericentriolar material 1 (PCM1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCo260085	XM_013366632.1 Latest version!	Columba livia pericentriolar material 1 (PCM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCo293160	XM_021285856.1 Latest version!	Columba livia pericentriolar material 1 (PCM1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCo293160	XM_021285855.1 Latest version!	Columba livia pericentriolar material 1 (PCM1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCo293160	XM_021285854.1 Latest version!	Columba livia pericentriolar material 1 (PCM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCo293160	XM_021285857.1 Latest version!	Columba livia pericentriolar material 1 (PCM1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCo293161	XM_021285858.1 Latest version!	Columba livia pericentriolar material 1 (PCM1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCo293162	XM_021285859.1 Latest version!	Columba livia pericentriolar material 1 (PCM1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCo293163	XM_021285860.1 Latest version!	Columba livia pericentriolar material 1 (PCM1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCo293164	XM_021285861.1 Latest version!	Columba livia pericentriolar material 1 (PCM1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCo293165	XM_021285862.1 Latest version!	Columba livia pericentriolar material 1 (PCM1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCo293166	XM_021285863.1 Latest version!	Columba livia pericentriolar material 1 (PCM1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCo293167	XM_021285864.1 Latest version!	Columba livia pericentriolar material 1 (PCM1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCo293168	XM_021285865.1 Latest version!	Columba livia pericentriolar material 1 (PCM1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCo293169	XM_021285866.1 Latest version!	Columba livia pericentriolar material 1 (PCM1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCo293170	XM_021285867.1 Latest version!	Columba livia pericentriolar material 1 (PCM1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCo293173	XM_021285870.1 Latest version!	Columba livia pericentriolar material 1 (PCM1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCo293174	XM_021285871.1 Latest version!	Columba livia pericentriolar material 1 (PCM1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00

PCM1 ( XM_013366633.1 ) cDNA ORF clone, Columba livia(rock pigeon) -> XP_013222087.1 Columba livia pericentriolar material 1 (PCM1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCo260086
Clone ID Related Accession (Same CDS sequence)	XM_013366633.1
Accession Version	XM_013366633.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-12
Organism	Columba livia(Rock pigeon)
Product	pericentriolar material 1 protein isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004973253.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Columba livia Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACAG GAGGTGGTCC CTTTGAAGAA GGCATGAATG ATCAAGACTT GCCTAGCTGG 
AGCAATGACA GCTTTGATGA CCGGCTGAAC AACACGGACT GGGGAAGTCA ACAGAAGAAA 
GCAAACAGAT CTTCAGAAAA AAACAAGAAA AAGCTTGGTG GGGAAGCTGA AACAAGGCTT 
ACTAATGATA TATCTCCAGA ATCCTCACCT GGAATAGGAC GACGAAAGAC CAGAACTCCT 
CATAGTTTTC CTCATGCTCG ATACATGACC CAGATGTCTG TTCCGGAGCA GGCTGAGCTA 
GAAAGGCTTA AACAAAGAAT AAACTTCAGT GATCTGGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGCA GGGCAACAGC TGCTAACAAC AAACGTCAAC TTAATGAAAA CAAAAAACCA 
TTCAACTTCC TGTCACTGCA GATAAACACT AACAAGAGCA AAGATCCTGC CTCATGTTCC 
CAAAAAAAGG AAAGTGGGGT GTCAGTCCAA CGTAAAGAGT TGTTTGGTGC TGCTCTGAGC 
AAGGATTTCT TGCAAAATTG TCAAGTCTCT GCTCAAGAAG ATGGAGGGGG AGAACCAGCT 
ATGGATAGTA GCCAGGCTAG AGATCCTCAA CAGGAGGCTA AAGAGGAGTT GGAGAACTTG 
AAGAAGCAGC ATGATTTATT GAAAAGGATG CTAAAACAGC AAGAGCAATT AAAGGCTCTT 
CAAGGAAGAC AGGCAGCTCT TCTTGCTTTG CAGCATAAAG CAGAGCAAGC AATTGCTGTC 
ATGGATGATT CTGTTGTAAC AGAGACTACA GGTAGTGTTT CAGGAGTAAG CCTTACATCA 
GAACTGAATG AAGAATTGAA TGACTTAATT CAGCGCTTTC ACAACCAACT TCATGATTCT 
CAGACACAGT CTGTACCAGA CAATAGAAGG CAAGCAGAAA GTCTTTCACT TACTAGAGAG 
GTTTCACAAA GCAGAAACTC TTCAATGTCT GAACGCCTGT CAGATGAGAA GGCGCAGCTT 
TTTAACAAGA TGCGAATGTT GCAGGGTAAA AAGCAAAAAA TGGACAAACT GTTAGGAGAA 
CTTCATACAC TTCGTGACCA ACATCTAAAT AACTCCTCCT TTTTTCCTGC TTCAGGTTCT 
CCTCAAAGAA GTATTGATCA AAGAAGTACA ACTTCAGCTG CTTCTGGTCC TGCAGGCATA 
GTAACTGTTG TCAATGGCGA ATCAAATAGC CTGGCATCTG CTCCCTATCC TCCTGATTCC 
CTGGTTTCTC AGAATGAGAG TGAAGAAGAT GACAATCTAA ATCCAACAGA AAAGCTTCAG 
AAACTAAATG AAGTTCGTAA GAGGCTGAAT GAGTTACGTG AGCTAGTTCA CTACTATGAG 
CAAACATCTG ATATGATGAC AGATTCTGTG AATGAAAACA CTAAGGAAGA AGAAGAAACC 
GAAGAATCAG AAAGTGATTC TGAACGTGAG GATCCACAGC CTGTTACAAA TATTAGAAAT 
CCTCAAGGAA TCAGTAGTTG GAGTGACATA AATAGCAACT CAAATGTACA GTGTGGAACT 
AATAACAGAG ATGGAAGACA TCTTAATACA GACTGTGAAA TAAATAACAG ATCTGCTGCT 
AACATAAGGA CTCTAAAAAT GTCGTCACCT TTAGACTGTC ATAATAGGGA GGATGACAAA 
GATATTGGCC TACCCCAATG TGAAGATGAA GAAGTGGAAG AAGATAGAGT TAGTGAAGAT 
TCTGCATCTA GTCACAGAAG CAGCCTGGGT GATACTGCTG GAGATGCTGA GTTTGAGCAG 
AAGATCAATA GGCTTATAGC TGCAAAACAG AAACTTAGAC AATTACAAAA CCTTGCTGCT 
ATGGTGCAGG ATGATGATCC AGCACCTCAG ACAACAATTG CAAATGCATC TAATATTGGT 
GACTTGTTTT TGGGTGAGAT GGAAGAGACA AAGCAGCAGC CAAACAATGT CCGAGCTAGT 
ACCAACAAAT TACAAAAAGA TGTAGAACTG AATGAAAAAG CAAGAGAGAA GTTTTATGAA 
GCTAAACTCC AGCAGCAGCA ACAGGAGCTT AAGCAGTTAC AAGAAGAAAG AAGAAAACTG 
ATTGAAATTC AAGAAAAAAT TCAGGTGTTA CAGAAAGCTT GTCCTGATCT TCAATTGTCA 
GCTGGCTTGG GTGACTGCAC AGCAAATAGA CAGACTTCAC CAGCAACATC AACTCCAGCC 
ATGAATGACA CAGCTGGCAA GCCTTCACTT GAATTTGATG AATCTGTACC AGTAGGCAAT 
GAGTTATGGT CTGAGATGAG AAGACATGAG ATTTTAAGAG AAGAATTGCG ACAAAGAAGA 
AAGCAACTGG AAGCTCTAAT GGCTGAGCAT CAGAGGAGGA AAGAGCTCGC AGAAACAATA 
TCTACTGTTG CTGCGTCTGT TAAAAGTGAA GGATCAGAAG CTCGGCGTAC TCCACAGCAG 
AGTAGGACTG AAAATAGGAC AATGGCTACC TGGGGAGGTT CTACGCAGTG TGCACTAGAT 
GAAGAGGATG GAGATGAAGA TGGTTATCTC TCTGATGGAG CTGGTCAGGC AGAAGAAGAT 
GATGATGATG CGTCAAGTGC GAATGACAGC TTCTCTGTTT ATCCCAATAA CAACGTACCA 
GAAAATGCAT ATTTTGTTAA AGAAAACAAG GATAGGTGGA AAAATTACCG TCCTCTTTCA 
GCAGATGGAA ATTATCGTCC ATTGTCTAAG GCCAGACAAC AGCAAAACAT AAGTATGCGA 
CGTCAGGAAA ACCTTCGGTG GATGTCTGAA CTTTCATATG TGGAAGAAAA GGAACGATGG 
CAAGAGCAGA TCAATCAGTT GAAGAAACAG CATGAATTTA GTGTCAGCAT TTGTCAAACT 
TTAATGCAGG ATCAGCAGAC CCTCTCTTGT CTTCTACAGA CTTTGCTTAC AGGCCCTTAC 
AGTATGATGC CCAATAATGT TGCATCTTCA CAAGTACATC TTATTATGCA TCAGCTAAAC 
CAGTGTTACA CTCAACTGAC ATGGCAGCAG AATAACGTCC AGAGGTTGAA ACAAATGTTA 
AATGATCTTG TGCGTCAGCA AGAAGAACAA CGGTATGAAG AGAAGTCATC AAGAAAGGAT 
AGAGGCAGTG GTGTACCCCC ACCTCCATCT CCTGTTTTCT GTCCGTTCAG CTTTCCTCCA 
CAACCTTTGA ACCTGTTTAA TGTACCAGGA TTTACTAATT TTTCTTCCTT TGCTCCAGAT 
ATTAACTATA ATCCAGTGTT TCCTTCTGGT TTTGGAGATT TTGCACACAA TATTTCTCCA 
CACAGCAGTG AGCAGCAGCA GCAACATCCT CTAGATCATA ATGCATCTGG GAAAACTGAG 
TATATGGCGT TCCCCAAACC TTTTGAAAGC AGTTCCTCTA ATGGAGCGGA AAAACAAAGG 
AGGAGTCACA GACAACCTGA AGAGGAAATG GAAAAAAGAT CAGCTTGGCT TAATGATAGC 
CAGGACGTGA AAAAAGATGA TCAGTCTCAG CTGAAAGCAA GTTTTGCTGT TTCAGTACAA 
AACATTGCTT CCAATCATAA AAATCAGTCT GATATGAGCA GAAGAAGAGA GTTTGATGAA 
GAGTCTTTGG AGAGTTTTAG TAGCATGCCT GATCCAGTAG ACCCCACTAC TGTGACAAAG 
ACATTTAAAT CTAGAAAAGC ATCAGCACAA GCAAGTTTGG CATCAAAAGA TAAAACACCT 
AAATCGAAGA ATAAGAGGAA GAGTTCTTCT CAGCTAAAAG GCAGAATTAC AAATATTGAT 
TATGAAAGTG CCAGTGCTTC TAGTGTGTGT GAACCCTGCA AGAGCAATAA AAGCAGACAC 
TCTGAAGAGG TTGGTCATGC AAAGGTGTTC AGCAAAAGGA ATCGGGAACA ACTGGAAAAA 
ATCATTAAAT ACAGTAGATC TACAGAAATG TCTTCAGAAA CTGGTAGTGA TCTTTCTATG 
TTTGAAGCTT TACGAGACAC AATTTATTCT GAAGTGGCAA CTCTTATTTC TCAAAATGAG 
TCTCGTCCCC ACTTTCTTAT TGAACTTTTC CATGAGCTTC AGCTGCTAAA TACAGATTAC 
CTGAGGCAAA GAGCTCTATA CGCTCTACAG GATATAGTGA CCAGACATTT ATCTGAGAAC 
AATGAAAAAG GGAAGTGCAC AAAATCACTG AATTCTGCAG CATGGATAGC ATCAAATTCT 
GAACTCACTC CCAGTGAAAG CCTTGCTTCT ACAGATGATG AAACTTTTGG CAAAAACTTT 
TCTACAGAAG CATGTCAAGA TTGTGAACAA AATGATGCAG ACAATGGGAG TAGCATGTCT 
ACATCTTCAA ATTTTGAACC CTTTGCCACT GATGACCTTG GCAACACAGT GATTCACTTA 
GATCAAGCTT TGGCTAGGAT GAGGGAGTAT GAGCGTATGA AAATTGAAGC TGAAAGTACC 
CTTGACTCTG AGGGCTGCTC TAGTAATTTT CAGGGTGCTT CTGCTGCTAA ATTAGAAGGA 
GGTCAAAGTG CCAGTGAATG TGTTCCTGTG CCACAGTCAA GTGAAGCTTC TGCTGTTCCA 
TGTCCTCATA TAGACACTCA GCAGCTTGAC CGGCAGATTA AAGCAATTAT GAAAGAGGTC 
ATTCCTTTTC TGAAGGAACA CATGGATGAA GTATGCTCTT CGCAGTTACT GACATCAGTA 
AGACGTATGG TCTTGACCCT TACACAACAA AATGATGAAA GTAAAGAGTT TGTGAAGTTC 
TTTCATAAGC AGCTTGGCAG TATACTTCAG GATTCACTGG CAAAATTTGC TGGTAGAAAA 
CTGAAAGATT GTGGAGAGGA TCTTCTAGTG GAGATCTCTG AAGTGTTATT TAATGAATTA 
GCCTTTTTTA AACTCATGCA AGACTTGGAC AACAACAGTA TTTCTGTAAA GCAGAGATGT 
AAAAGAAAAA TAGAAGCTAC TGAAGTGATA CAGTCGTATG CTAAAGAGGC AAAAAAAGGT 
CTCCAGGTGG ATGTTTGTTC ATCTGCTGAA GATGTCGATG AGGACAAAGA CAAGGATGAG 
ACTGAAACTG TTAAACAAGT ACAGGACTCA GAAATGTATG ATGGGAATAG AGTTCCTGAA 
AGTATTAGGT CTGATGCATC TGATCAGGAG GAAGATGATG AAAGTGAAAG CGGTCCAGTG 
GCAATAAGTT TATCAAAAGC AGAAACCCAA GCTCTGACTA ACTATGGTAG TGGAGAAGAT 
GAGAATGAAG ATGAAGAAAT AGAATTTGAG GAAGGACCTG TTGATGTGCA GACGTCATTA 
CAAGCCAGCA GTGAAACAAC AACTGAAAAT GAACAGAATT CAAACCAAGA ACAAAGTAAG 
ACAAAAAGCA GTGAGATTCT GTCATCAGAA CAAGAATCTG TTTATGCTAA AGGTGAACAA 
CATGTGGCTA CAATTTTGCC TTATTACCTC AATGTAATGG AGAATGCACC GCCTTTAACG 
GTCAATACCC CAGAATCTTT TATAACAGAC AGTATGAAAA CAGAAGAATC AAGTTCATCT 
TTACCAGTAA ATGAAACTCA AACACTGGAT ACAACGTGTG TAGGAAACAA ATCTGCTGCA 
AGTTCTGAAA GCTCCATGGC TGGCAGCCCT GATACAGAGT CACCTGTGTT AGTGAATGAA 
TATGAAGCTG GTTCTGGAAA TGTAAGTCAA AGATCTGATG AAGATGACTT TGTGAAAGTT 
GAAGACTTGC CCCTTAAACT TGCAGTGTAT TCAGAGGCAG ATTTAATGAA GAAAATGGCA 
ATGGAGGCCC AAACGAACAG TTTGTCTGAT GAATTACTGG GTGGAGGTGG AGCTCAAGAT 
CAAGAATTAG TAGGAGATGC CCAAACTTTG AAAGAACCTG AAACTTTTGG AGTTCAAAGT 
GCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013222087.1
CDS	96..5981
Translation	MATGGGPFEEGMNDQDLPSWSNDSFDDRLNNTDWGSQQKKANRSSEKNKKKLGGEAETRLTNDISPESSPGIGRRKTRTPHSFPHARYMTQMSVPEQAELERLKQRINFSDLDQRSIGSDSQGRATAANNKRQLNENKKPFNFLSLQINTNKSKDPASCSQKKESGVSVQRKELFGAALSKDFLQNCQVSAQEDGGGEPAMDSSQARDPQQEAKEELENLKKQHDLLKRMLKQQEQLKALQGRQAALLALQHKAEQAIAVMDDSVVTETTGSVSGVSLTSELNEELNDLIQRFHNQLHDSQTQSVPDNRRQAESLSLTREVSQSRNSSMSERLSDEKAQLFNKMRMLQGKKQKMDKLLGELHTLRDQHLNNSSFFPASGSPQRSIDQRSTTSAASGPAGIVTVVNGESNSLASAPYPPDSLVSQNESEEDDNLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDSVNENTKEEEETEESESDSEREDPQPVTNIRNPQGISSWSDINSNSNVQCGTNNRDGRHLNTDCEINNRSAANIRTLKMSSPLDCHNREDDKDIGLPQCEDEEVEEDRVSEDSASSHRSSLGDTAGDAEFEQKINRLIAAKQKLRQLQNLAAMVQDDDPAPQTTIANASNIGDLFLGEMEETKQQPNNVRASTNKLQKDVELNEKAREKFYEAKLQQQQQELKQLQEERRKLIEIQEKIQVLQKACPDLQLSAGLGDCTANRQTSPATSTPAMNDTAGKPSLEFDESVPVGNELWSEMRRHEILREELRQRRKQLEALMAEHQRRKELAETISTVAASVKSEGSEARRTPQQSRTENRTMATWGGSTQCALDEEDGDEDGYLSDGAGQAEEDDDDASSANDSFSVYPNNNVPENAYFVKENKDRWKNYRPLSADGNYRPLSKARQQQNISMRRQENLRWMSELSYVEEKERWQEQINQLKKQHEFSVSICQTLMQDQQTLSCLLQTLLTGPYSMMPNNVASSQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNDLVRQQEEQRYEEKSSRKDRGSGVPPPPSPVFCPFSFPPQPLNLFNVPGFTNFSSFAPDINYNPVFPSGFGDFAHNISPHSSEQQQQHPLDHNASGKTEYMAFPKPFESSSSNGAEKQRRSHRQPEEEMEKRSAWLNDSQDVKKDDQSQLKASFAVSVQNIASNHKNQSDMSRRREFDEESLESFSSMPDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKNKRKSSSQLKGRITNIDYESASASSVCEPCKSNKSRHSEEVGHAKVFSKRNREQLEKIIKYSRSTEMSSETGSDLSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHLSENNEKGKCTKSLNSAAWIASNSELTPSESLASTDDETFGKNFSTEACQDCEQNDADNGSSMSTSSNFEPFATDDLGNTVIHLDQALARMREYERMKIEAESTLDSEGCSSNFQGASAAKLEGGQSASECVPVPQSSEASAVPCPHIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSISVKQRCKRKIEATEVIQSYAKEAKKGLQVDVCSSAEDVDEDKDKDETETVKQVQDSEMYDGNRVPESIRSDASDQEEDDESESGPVAISLSKAETQALTNYGSGEDENEDEEIEFEEGPVDVQTSLQASSETTTENEQNSNQEQSKTKSSEILSSEQESVYAKGEQHVATILPYYLNVMENAPPLTVNTPESFITDSMKTEESSSSLPVNETQTLDTTCVGNKSAASSESSMAGSPDTESPVLVNEYEAGSGNVSQRSDEDDFVKVEDLPLKLAVYSEADLMKKMAMEAQTNSLSDELLGGGGAQDQELVGDAQTLKEPETFGVQSA

Target ORF information:

RefSeq Version	XM_013366633.1
Organism	Columba livia(Rock pigeon)
Definition	Columba livia pericentriolar material 1 (PCM1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013366633.1

ORF Insert Sequence:

ATGGCAACAG GAGGTGGTCC CTTTGAAGAA GGCATGAATG ATCAAGACTT GCCTAGCTGG 
AGCAATGACA GCTTTGATGA CCGGCTGAAC AACACGGACT GGGGAAGTCA ACAGAAGAAA 
GCAAACAGAT CTTCAGAAAA AAACAAGAAA AAGCTTGGTG GGGAAGCTGA AACAAGGCTT 
ACTAATGATA TATCTCCAGA ATCCTCACCT GGAATAGGAC GACGAAAGAC CAGAACTCCT 
CATAGTTTTC CTCATGCTCG ATACATGACC CAGATGTCTG TTCCGGAGCA GGCTGAGCTA 
GAAAGGCTTA AACAAAGAAT AAACTTCAGT GATCTGGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGCA GGGCAACAGC TGCTAACAAC AAACGTCAAC TTAATGAAAA CAAAAAACCA 
TTCAACTTCC TGTCACTGCA GATAAACACT AACAAGAGCA AAGATCCTGC CTCATGTTCC 
CAAAAAAAGG AAAGTGGGGT GTCAGTCCAA CGTAAAGAGT TGTTTGGTGC TGCTCTGAGC 
AAGGATTTCT TGCAAAATTG TCAAGTCTCT GCTCAAGAAG ATGGAGGGGG AGAACCAGCT 
ATGGATAGTA GCCAGGCTAG AGATCCTCAA CAGGAGGCTA AAGAGGAGTT GGAGAACTTG 
AAGAAGCAGC ATGATTTATT GAAAAGGATG CTAAAACAGC AAGAGCAATT AAAGGCTCTT 
CAAGGAAGAC AGGCAGCTCT TCTTGCTTTG CAGCATAAAG CAGAGCAAGC AATTGCTGTC 
ATGGATGATT CTGTTGTAAC AGAGACTACA GGTAGTGTTT CAGGAGTAAG CCTTACATCA 
GAACTGAATG AAGAATTGAA TGACTTAATT CAGCGCTTTC ACAACCAACT TCATGATTCT 
CAGACACAGT CTGTACCAGA CAATAGAAGG CAAGCAGAAA GTCTTTCACT TACTAGAGAG 
GTTTCACAAA GCAGAAACTC TTCAATGTCT GAACGCCTGT CAGATGAGAA GGCGCAGCTT 
TTTAACAAGA TGCGAATGTT GCAGGGTAAA AAGCAAAAAA TGGACAAACT GTTAGGAGAA 
CTTCATACAC TTCGTGACCA ACATCTAAAT AACTCCTCCT TTTTTCCTGC TTCAGGTTCT 
CCTCAAAGAA GTATTGATCA AAGAAGTACA ACTTCAGCTG CTTCTGGTCC TGCAGGCATA 
GTAACTGTTG TCAATGGCGA ATCAAATAGC CTGGCATCTG CTCCCTATCC TCCTGATTCC 
CTGGTTTCTC AGAATGAGAG TGAAGAAGAT GACAATCTAA ATCCAACAGA AAAGCTTCAG 
AAACTAAATG AAGTTCGTAA GAGGCTGAAT GAGTTACGTG AGCTAGTTCA CTACTATGAG 
CAAACATCTG ATATGATGAC AGATTCTGTG AATGAAAACA CTAAGGAAGA AGAAGAAACC 
GAAGAATCAG AAAGTGATTC TGAACGTGAG GATCCACAGC CTGTTACAAA TATTAGAAAT 
CCTCAAGGAA TCAGTAGTTG GAGTGACATA AATAGCAACT CAAATGTACA GTGTGGAACT 
AATAACAGAG ATGGAAGACA TCTTAATACA GACTGTGAAA TAAATAACAG ATCTGCTGCT 
AACATAAGGA CTCTAAAAAT GTCGTCACCT TTAGACTGTC ATAATAGGGA GGATGACAAA 
GATATTGGCC TACCCCAATG TGAAGATGAA GAAGTGGAAG AAGATAGAGT TAGTGAAGAT 
TCTGCATCTA GTCACAGAAG CAGCCTGGGT GATACTGCTG GAGATGCTGA GTTTGAGCAG 
AAGATCAATA GGCTTATAGC TGCAAAACAG AAACTTAGAC AATTACAAAA CCTTGCTGCT 
ATGGTGCAGG ATGATGATCC AGCACCTCAG ACAACAATTG CAAATGCATC TAATATTGGT 
GACTTGTTTT TGGGTGAGAT GGAAGAGACA AAGCAGCAGC CAAACAATGT CCGAGCTAGT 
ACCAACAAAT TACAAAAAGA TGTAGAACTG AATGAAAAAG CAAGAGAGAA GTTTTATGAA 
GCTAAACTCC AGCAGCAGCA ACAGGAGCTT AAGCAGTTAC AAGAAGAAAG AAGAAAACTG 
ATTGAAATTC AAGAAAAAAT TCAGGTGTTA CAGAAAGCTT GTCCTGATCT TCAATTGTCA 
GCTGGCTTGG GTGACTGCAC AGCAAATAGA CAGACTTCAC CAGCAACATC AACTCCAGCC 
ATGAATGACA CAGCTGGCAA GCCTTCACTT GAATTTGATG AATCTGTACC AGTAGGCAAT 
GAGTTATGGT CTGAGATGAG AAGACATGAG ATTTTAAGAG AAGAATTGCG ACAAAGAAGA 
AAGCAACTGG AAGCTCTAAT GGCTGAGCAT CAGAGGAGGA AAGAGCTCGC AGAAACAATA 
TCTACTGTTG CTGCGTCTGT TAAAAGTGAA GGATCAGAAG CTCGGCGTAC TCCACAGCAG 
AGTAGGACTG AAAATAGGAC AATGGCTACC TGGGGAGGTT CTACGCAGTG TGCACTAGAT 
GAAGAGGATG GAGATGAAGA TGGTTATCTC TCTGATGGAG CTGGTCAGGC AGAAGAAGAT 
GATGATGATG CGTCAAGTGC GAATGACAGC TTCTCTGTTT ATCCCAATAA CAACGTACCA 
GAAAATGCAT ATTTTGTTAA AGAAAACAAG GATAGGTGGA AAAATTACCG TCCTCTTTCA 
GCAGATGGAA ATTATCGTCC ATTGTCTAAG GCCAGACAAC AGCAAAACAT AAGTATGCGA 
CGTCAGGAAA ACCTTCGGTG GATGTCTGAA CTTTCATATG TGGAAGAAAA GGAACGATGG 
CAAGAGCAGA TCAATCAGTT GAAGAAACAG CATGAATTTA GTGTCAGCAT TTGTCAAACT 
TTAATGCAGG ATCAGCAGAC CCTCTCTTGT CTTCTACAGA CTTTGCTTAC AGGCCCTTAC 
AGTATGATGC CCAATAATGT TGCATCTTCA CAAGTACATC TTATTATGCA TCAGCTAAAC 
CAGTGTTACA CTCAACTGAC ATGGCAGCAG AATAACGTCC AGAGGTTGAA ACAAATGTTA 
AATGATCTTG TGCGTCAGCA AGAAGAACAA CGGTATGAAG AGAAGTCATC AAGAAAGGAT 
AGAGGCAGTG GTGTACCCCC ACCTCCATCT CCTGTTTTCT GTCCGTTCAG CTTTCCTCCA 
CAACCTTTGA ACCTGTTTAA TGTACCAGGA TTTACTAATT TTTCTTCCTT TGCTCCAGAT 
ATTAACTATA ATCCAGTGTT TCCTTCTGGT TTTGGAGATT TTGCACACAA TATTTCTCCA 
CACAGCAGTG AGCAGCAGCA GCAACATCCT CTAGATCATA ATGCATCTGG GAAAACTGAG 
TATATGGCGT TCCCCAAACC TTTTGAAAGC AGTTCCTCTA ATGGAGCGGA AAAACAAAGG 
AGGAGTCACA GACAACCTGA AGAGGAAATG GAAAAAAGAT CAGCTTGGCT TAATGATAGC 
CAGGACGTGA AAAAAGATGA TCAGTCTCAG CTGAAAGCAA GTTTTGCTGT TTCAGTACAA 
AACATTGCTT CCAATCATAA AAATCAGTCT GATATGAGCA GAAGAAGAGA GTTTGATGAA 
GAGTCTTTGG AGAGTTTTAG TAGCATGCCT GATCCAGTAG ACCCCACTAC TGTGACAAAG 
ACATTTAAAT CTAGAAAAGC ATCAGCACAA GCAAGTTTGG CATCAAAAGA TAAAACACCT 
AAATCGAAGA ATAAGAGGAA GAGTTCTTCT CAGCTAAAAG GCAGAATTAC AAATATTGAT 
TATGAAAGTG CCAGTGCTTC TAGTGTGTGT GAACCCTGCA AGAGCAATAA AAGCAGACAC 
TCTGAAGAGG TTGGTCATGC AAAGGTGTTC AGCAAAAGGA ATCGGGAACA ACTGGAAAAA 
ATCATTAAAT ACAGTAGATC TACAGAAATG TCTTCAGAAA CTGGTAGTGA TCTTTCTATG 
TTTGAAGCTT TACGAGACAC AATTTATTCT GAAGTGGCAA CTCTTATTTC TCAAAATGAG 
TCTCGTCCCC ACTTTCTTAT TGAACTTTTC CATGAGCTTC AGCTGCTAAA TACAGATTAC 
CTGAGGCAAA GAGCTCTATA CGCTCTACAG GATATAGTGA CCAGACATTT ATCTGAGAAC 
AATGAAAAAG GGAAGTGCAC AAAATCACTG AATTCTGCAG CATGGATAGC ATCAAATTCT 
GAACTCACTC CCAGTGAAAG CCTTGCTTCT ACAGATGATG AAACTTTTGG CAAAAACTTT 
TCTACAGAAG CATGTCAAGA TTGTGAACAA AATGATGCAG ACAATGGGAG TAGCATGTCT 
ACATCTTCAA ATTTTGAACC CTTTGCCACT GATGACCTTG GCAACACAGT GATTCACTTA 
GATCAAGCTT TGGCTAGGAT GAGGGAGTAT GAGCGTATGA AAATTGAAGC TGAAAGTACC 
CTTGACTCTG AGGGCTGCTC TAGTAATTTT CAGGGTGCTT CTGCTGCTAA ATTAGAAGGA 
GGTCAAAGTG CCAGTGAATG TGTTCCTGTG CCACAGTCAA GTGAAGCTTC TGCTGTTCCA 
TGTCCTCATA TAGACACTCA GCAGCTTGAC CGGCAGATTA AAGCAATTAT GAAAGAGGTC 
ATTCCTTTTC TGAAGGAACA CATGGATGAA GTATGCTCTT CGCAGTTACT GACATCAGTA 
AGACGTATGG TCTTGACCCT TACACAACAA AATGATGAAA GTAAAGAGTT TGTGAAGTTC 
TTTCATAAGC AGCTTGGCAG TATACTTCAG GATTCACTGG CAAAATTTGC TGGTAGAAAA 
CTGAAAGATT GTGGAGAGGA TCTTCTAGTG GAGATCTCTG AAGTGTTATT TAATGAATTA 
GCCTTTTTTA AACTCATGCA AGACTTGGAC AACAACAGTA TTTCTGTAAA GCAGAGATGT 
AAAAGAAAAA TAGAAGCTAC TGAAGTGATA CAGTCGTATG CTAAAGAGGC AAAAAAAGGT 
CTCCAGGTGG ATGTTTGTTC ATCTGCTGAA GATGTCGATG AGGACAAAGA CAAGGATGAG 
ACTGAAACTG TTAAACAAGT ACAGGACTCA GAAATGTATG ATGGGAATAG AGTTCCTGAA 
AGTATTAGGT CTGATGCATC TGATCAGGAG GAAGATGATG AAAGTGAAAG CGGTCCAGTG 
GCAATAAGTT TATCAAAAGC AGAAACCCAA GCTCTGACTA ACTATGGTAG TGGAGAAGAT 
GAGAATGAAG ATGAAGAAAT AGAATTTGAG GAAGGACCTG TTGATGTGCA GACGTCATTA 
CAAGCCAGCA GTGAAACAAC AACTGAAAAT GAACAGAATT CAAACCAAGA ACAAAGTAAG 
ACAAAAAGCA GTGAGATTCT GTCATCAGAA CAAGAATCTG TTTATGCTAA AGGTGAACAA 
CATGTGGCTA CAATTTTGCC TTATTACCTC AATGTAATGG AGAATGCACC GCCTTTAACG 
GTCAATACCC CAGAATCTTT TATAACAGAC AGTATGAAAA CAGAAGAATC AAGTTCATCT 
TTACCAGTAA ATGAAACTCA AACACTGGAT ACAACGTGTG TAGGAAACAA ATCTGCTGCA 
AGTTCTGAAA GCTCCATGGC TGGCAGCCCT GATACAGAGT CACCTGTGTT AGTGAATGAA 
TATGAAGCTG GTTCTGGAAA TGTAAGTCAA AGATCTGATG AAGATGACTT TGTGAAAGTT 
GAAGACTTGC CCCTTAAACT TGCAGTGTAT TCAGAGGCAG ATTTAATGAA GAAAATGGCA 
ATGGAGGCCC AAACGAACAG TTTGTCTGAT GAATTACTGG GTGGAGGTGG AGCTCAAGAT 
CAAGAATTAG TAGGAGATGC CCAAACTTTG AAAGAACCTG AAACTTTTGG AGTTCAAAGT 
GCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_021285868.1 ) cDNA ORF clone, Columba livia(rock pigeon) -> XP_021141543.1 Columba livia pericentriolar material 1 (PCM1), transcript variant X15, mRNA.

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CloneID	OCo293171
Clone ID Related Accession (Same CDS sequence)	XM_021285868.1
Accession Version	XM_021285868.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5943bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-23
Organism	Columba livia(rock pigeon)
Product	pericentriolar material 1 protein isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004973253.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Columba livia Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACAG GAGGTGGTCC CTTTGAAGAA GGCATGAATG ATCAAGACTT GCCTAGCTGG 
AGCAATGACA GCTTTGATGA CCGGCTGAAC AACACGGACT GGGGAAGTCA ACAGAAGAAA 
GCAAACAGAT CTTCAGAAAA AAACAAGAAA AAGCTTGGTG GGGAAGCTGA AACAAGGCTT 
ACTAATGATA TATCTCCAGA ATCCTCACCT GGAATAGGAC GACGAAAGAC CAGAACTCCT 
CATAGTTTTC CTCATGCTCG ATACATGACC CAGATGTCTG TTCCGGAGCA GGCTGAGCTA 
GAAAGGCTTA AACAAAGAAT AAACTTCAGT GATCTGGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGCA GGGCAACAGC TGCTAACAAC AAACGTCAAC TTAATGAAAA CAAAAAACCA 
TTCAACTTCC TGTCACTGCA GATAAACACT AACAAGAGCA AAGATCCTGC CTCATGTTCC 
CAAAAAAAGG AAAGTGGGGT GTCAGTCCAA CGTAAAGAGT TGTTTGGTGC TGCTCTGAGC 
AAGGATTTCT TGCAAAATTG TCAAGTCTCT GCTCAAGAAG ATGGAGGGGG AGAACCAGCT 
ATGGATAGTA GCCAGGCTAG AGATCCTCAA CAGGAGGCTA AAGAGGAGTT GGAGAACTTG 
AAGAAGCAGC ATGATTTATT GAAAAGGATG CTAAAACAGC AAGAGCAATT AAAGGCTCTT 
CAAGGAAGAC AGGCAGCTCT TCTTGCTTTG CAGCATAAAG CAGAGCAAGC AATTGCTGTC 
ATGGATGATT CTGTTGTAAC AGAGACTACA GGTAGTGTTT CAGGAGTAAG CCTTACATCA 
GAACTGAATG AAGAATTGAA TGACTTAATT CAGCGCTTTC ACAACCAACT TCATGATTCT 
CAGACACAGT CTGTACCAGA CAATAGAAGG CAAGCAGAAA GTCTTTCACT TACTAGAGAG 
GTTTCACAAA GCAGAAACTC TTCAATGTCT GAACGCCTGT CAGATGAGAA GGCGCAGCTT 
TTTAACAAGA TGCGAATGTT GCAGGGTAAA AAGCAAAAAA TGGACAAACT GTTAGGAGAA 
CTTCATACAC TTCGTGACCA ACATCTAAAT AACTCCTCCT TTTTTCCTGC TTCAGGTTCT 
CCTCAAAGAA GTATTGATCA AAGAAGTACA ACTTCAGCTG CTTCTGGTCC TGCAGGCATA 
GTAACTGTTG TCAATGGCGA ATCAAATAGC CTGGCATCTG CTCCCTATCC TCCTGATTCC 
CTGGTTTCTC AGAATGAGAG TGAAGAAGAT GACAATCTAA ATCCAACAGA AAAGCTTCAG 
AAACTAAATG AAGTTCGTAA GAGGCTGAAT GAGTTACGTG AGCTAGTTCA CTACTATGAG 
CAAACATCTG ATATGATGAC AGATTCTGTG AATGAAAACA CTAAGGAAGA AGAAGAAACC 
GAAGAATCAG AAAGTGATTC TGAACGTGAG GATCCACAGC CTGTTACAAA TATTAGAAAT 
CCTCAAGGAA TCAGTAGTTG GAGTGACATA AATAGCAACT CAAATGTACA GTGTGGAACT 
AATAACAGAG ATGGAAGACA TCTTAATACA GACTGTGAAA TAAATAACAG ATCTGCTGCT 
AACATAAGGA CTCTAAAAAT GTCGTCACCT TTAGACTGTC ATAATAGGGA GGATGACAAA 
GATATTGGCC TACCCCAATG TGAAGATGAA GAAGTGGAAG AAGATAGAGT TAGTGAAGAT 
TCTGCATCTA GTCACAGAAG CAGCCTGGGT GATACTGCTG GAGATGCTGA GTTTGAGCAG 
AAGATCAATA GGCTTATAGC TGCAAAACAG AAACTTAGAC AATTACAAAA CCTTGCTGCT 
ATGGTGCAGG ATGATGATCC AGCACCTCAG ACAACAATTG CAAATGCATC TAATATTGGT 
GACTTGTTTT TGGGTGAGAT GGAAGAGACA AAGCAGCAGC CAAACAATGT CCGAGCTAGT 
ACCAACAAAT TACAAAAAGA TGTAGAACTG AATGAAAAAG CAAGAGAGAA GTTTTATGAA 
GCTAAACTCC AGCAGCAGCA ACAGGAGCTT AAGCAGTTAC AAGAAGAAAG AAGAAAACTG 
ATTGAAATTC AAGAAAAAAT TCAGGTGTTA CAGAAAGCTT GTCCTGATCT TCAATTGTCA 
GCTGGCTTGG GTGACTGCAC AGCAAATAGA CAGACTTCAC CAGCAACATC AACTCCAGCC 
ATGAATGACA CAGCTGGCAA GCCTTCACTT GAATTTGATG AATCTGTACC AGTAGGCAAT 
GAGTTATGGT CTGAGATGAG AAGACATGAG ATTTTAAGAG AAGAATTGCG ACAAAGAAGA 
AAGCAACTGG AAGCTCTAAT GGCTGAGCAT CAGAGGAGGA AAGAGCTCGC AGAAACAATA 
TCTACTGTTG CTGCGTCTGT TAAAAGTGAA GGATCAGAAG CTCGGCGTAC TCCACAGCAG 
AGTAGGACTG AAAATAGGAC AATGGCTACC TGGGGAGGTT CTACGCAGTG TGCACTAGAT 
GAAGAGGATG GAGATGAAGA TGGTTATCTC TCTGATGGAG CTGGTCAGGC AGAAGAAGAT 
GATGATGATG CGTCAAGTGC GAATGACAGC TTCTCTGTTT ATCCCAATAA CAACGTACCA 
GAAAATGCAT ATTTTGTTAA AGAAAACAAG GATAGGTGGA AAAATTACCG TCCTCTTTCA 
GCAGATGGAA ATTATCGTCC ATTGTCTAAG GCCAGACAAC AGCAAAACAT AAGTATGCGA 
CGTCAGGAAA ACCTTCGGTG GATGTCTGAA CTTTCATATG TGGAAGAAAA GGAACGATGG 
CAAGAGCAGA TCAATCAGTT GAAGAAACAG CATGAATTTA GTGTCAGCAT TTGTCAAACT 
TTAATGCAGG ATCAGCAGAC CCTCTCTTGT CTTCTACAGA CTTTGCTTAC AGGCCCTTAC 
AGTATGATGC CCAATAATGT TGCATCTTCA CAAGTACATC TTATTATGCA TCAGCTAAAC 
CAGTGTTACA CTCAACTGAC ATGGCAGCAG AATAACGTCC AGAGGTTGAA ACAAATGTTA 
AATGATCTTG TGCGTCAGCA AGAAGAACAA CGGTATGAAG AGAAGTCATC AAGAAAGGAT 
AGAGGCAGTG GTGTACCCCC ACCTCCATCT CCTGTTTTCT GTCCGTTCAG CTTTCCTCCA 
CAACCTTTGA ACCTGTTTAA TGTACCAGGA TTTACTAATT TTTCTTCCTT TGCTCCAGAT 
ATTAACTATA ATCCAGTGTT TCCTTCTGGT TTTGGAGATT TTGCACACAA TATTTCTCCA 
CACAGCAGTG AGCAGCAGCA GCAACATCCT CTAGATCATA ATGCATCTGG GAAAACTGAG 
TATATGGCGT TCCCCAAACC TTTTGAAAGC AGTTCCTCTA ATGGAGCGGA AAAACAAAGG 
AGGAGTCACA GACAACCTGA AGAGGAAATG GAAAAAAGAT CAGCTTGGCT TAATGATAGC 
CAGGACGTGA AAAAAGATGA TCAGTCTCAG CTGAAAGCAA GTTTTGCTGT TTCAGTACAA 
AACATTGCTT CCAATCATAA AAATCAGTCT GATATGAGCA GAAGAAGAGA GTTTGATGAA 
GAGTCTTTGG AGAGTTTTAG TAGCATGCCT GATCCAGTAG ACCCCACTAC TGTGACAAAG 
ACATTTAAAT CTAGAAAAGC ATCAGCACAA GCAAGTTTGG CATCAAAAGA TAAAACACCT 
AAATCGAAGA ATAAGAGGAA GAGTTCTTCT CAGCTAAAAG GCAGAATTAC AAATATTGAT 
TATGAAAGTG CCAGTGCTTC TAGTGTGTGT GAACCCTGCA AGAGCAATAA AAGCAGACAC 
TCTGAAGAGG TTGGTCATGC AAAGGTGTTC AGCAAAAGGA ATCGGGAACA ACTGGAAAAA 
ATCATTAAAT ACAGTAGATC TACAGAAATG TCTTCAGCGC ATGCTAGGAG AATTCTGCAG 
CAGTCTAACA GAAATGCATG CATTGAAGCG CCAGAAACTG GTAGTGATCT TTCTATGTTT 
GAAGCTTTAC GAGACACAAT TTATTCTGAA GTGGCAACTC TTATTTCTCA AAATGAGTCT 
CGTCCCCACT TTCTTATTGA ACTTTTCCAT GAGCTTCAGC TGCTAAATAC AGATTACCTG 
AGGCAAAGAG CTCTATACGC TCTACAGGAT ATAGTGACCA GACATTTATC TGAGAACAAT 
GAAAAAGGGA AGTGCACAAA ATCACTGAAT TCTGCAGCAT GGATAGCATC AAATTCTGAA 
CTCACTCCCA GTGAAAGCCT TGCTTCTACA GATGATGAAA CTTTTGGCAA AAACTTTTCT 
ACAGAAGCAT GTCAAGATTG TGAACAAAAT GATGCAGACA ATGGGAGTAG CATGTCTACA 
TCTTCAAATT TTGAACCCTT TGCCACTGAT GACCTTGGCA ACACAGTGAT TCACTTAGAT 
CAAGCTTTGG CTAGGATGAG GGAGTATGAG CGTATGAAAA TTGAAGCTGA AAGTACCCTT 
GACTCTGAGG GCTGCTCTAG TAATTTTCAG GGTGCTTCTG CTGCTAAATT AGAAGGAGGT 
CAAAGTGCCA GTGAATGTGT TCCTGTGCCA CAGTCAAGTG AAGCTTCTGC TGTTCCATGT 
CCTCATATAG ACACTCAGCA GCTTGACCGG CAGATTAAAG CAATTATGAA AGAGGTCATT 
CCTTTTCTGA AGGAACACAT GGATGAAGTA TGCTCTTCGC AGTTACTGAC ATCAGTAAGA 
CGTATGGTCT TGACCCTTAC ACAACAAAAT GATGAAAGTA AAGAGTTTGT GAAGTTCTTT 
CATAAGCAGC TTGGCAGTAT ACTTCAGGAT TCACTGGCAA AATTTGCTGG TAGAAAACTG 
AAAGATTGTG GAGAGGATCT TCTAGTGGAG ATCTCTGAAG TGTTATTTAA TGAATTAGCC 
TTTTTTAAAC TCATGCAAGA CTTGGACAAC AACAGTATTT CTGTAAAGCA GAGATGTAAA 
AGAAAAATAG AAGCTACTGA AGTGATACAG TCGTATGCTA AAGAGGCAAA AAAAGGTCTC 
CAGGTGGATG TTTGTTCATC TGCTGAAGAT GTCGATGAGG ACAAAGACAA GGATGAGACT 
GAAACTGTTA AACAAGTACA GGACTCAGAA ATGTATGATG GGAATAGAGT TCCTGAAAGT 
ATTAGGTCTG ATGCATCTGA TCAGGAGGAA GATGATGAAA GTGAAAGCGG TCCAGTGGCA 
ATAAGTTTAT CAAAAGCAGA AACCCAAGCT CTGACTAACT ATGGTAGTGG AGAAGATGAG 
AATGAAGATG AAGAAATAGA ATTTGAGGAA GGACCTGTTG ATGTGCAGAC GTCATTACAA 
GCCAGCAGTG AAACAACAAC TGAAAATGAA CAGAATTCAA ACCAAGAACA AAGTAAGACA 
AAAAGCAGTG AGATTCTGTC ATCAGAACAA GAATCTGTTT ATGCTAAAGG TGAACAACAT 
GTGGCTACAA TTTTGCCTTA TTACCTCAAT GTAATGGAGA ATGCACCGCC TTTAACGGTC 
AATACCCCAG AATCTTTTAT AACAGACAGT ATGAAAACAG AAGAATCAAG TTCATCTTTA 
CCAGTAAATG AAACTCAAAC ACTGGATACA ACGTGTGTAG GAAACAAATC TGCTGCAAGT 
TCTGAAAGCT CCATGGCTGG CAGCCCTGAT ACAGAGTCAC CTGTGTTAGT GAATGAATAT 
GAAGCTGGTT CTGGAAATGT AAGTCAAAGA TCTGATGAAG ATGACTTTGT GAAAGTTGAA 
GACTTGCCCC TTAAACTTGC AGTGTATTCA GAGGCAGATT TAATGAAGAA AATGGCAATG 
GAGGCCCAAA CGAACAGTTT GTCTGATGAA TTACTGGGTG GAGGTGGAGC TCAAGATCAA 
GAATTAGTAG GAGATGCCCA AACTTTGAAA GAACCTGAAA CTTTTGGAGT TCAAAGTGCG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021141543.1
CDS	140..6082
Translation	MATGGGPFEEGMNDQDLPSWSNDSFDDRLNNTDWGSQQKKANRSSEKNKKKLGGEAETRLTNDISPESSPGIGRRKTRTPHSFPHARYMTQMSVPEQAELERLKQRINFSDLDQRSIGSDSQGRATAANNKRQLNENKKPFNFLSLQINTNKSKDPASCSQKKESGVSVQRKELFGAALSKDFLQNCQVSAQEDGGGEPAMDSSQARDPQQEAKEELENLKKQHDLLKRMLKQQEQLKALQGRQAALLALQHKAEQAIAVMDDSVVTETTGSVSGVSLTSELNEELNDLIQRFHNQLHDSQTQSVPDNRRQAESLSLTREVSQSRNSSMSERLSDEKAQLFNKMRMLQGKKQKMDKLLGELHTLRDQHLNNSSFFPASGSPQRSIDQRSTTSAASGPAGIVTVVNGESNSLASAPYPPDSLVSQNESEEDDNLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDSVNENTKEEEETEESESDSEREDPQPVTNIRNPQGISSWSDINSNSNVQCGTNNRDGRHLNTDCEINNRSAANIRTLKMSSPLDCHNREDDKDIGLPQCEDEEVEEDRVSEDSASSHRSSLGDTAGDAEFEQKINRLIAAKQKLRQLQNLAAMVQDDDPAPQTTIANASNIGDLFLGEMEETKQQPNNVRASTNKLQKDVELNEKAREKFYEAKLQQQQQELKQLQEERRKLIEIQEKIQVLQKACPDLQLSAGLGDCTANRQTSPATSTPAMNDTAGKPSLEFDESVPVGNELWSEMRRHEILREELRQRRKQLEALMAEHQRRKELAETISTVAASVKSEGSEARRTPQQSRTENRTMATWGGSTQCALDEEDGDEDGYLSDGAGQAEEDDDDASSANDSFSVYPNNNVPENAYFVKENKDRWKNYRPLSADGNYRPLSKARQQQNISMRRQENLRWMSELSYVEEKERWQEQINQLKKQHEFSVSICQTLMQDQQTLSCLLQTLLTGPYSMMPNNVASSQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNDLVRQQEEQRYEEKSSRKDRGSGVPPPPSPVFCPFSFPPQPLNLFNVPGFTNFSSFAPDINYNPVFPSGFGDFAHNISPHSSEQQQQHPLDHNASGKTEYMAFPKPFESSSSNGAEKQRRSHRQPEEEMEKRSAWLNDSQDVKKDDQSQLKASFAVSVQNIASNHKNQSDMSRRREFDEESLESFSSMPDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKNKRKSSSQLKGRITNIDYESASASSVCEPCKSNKSRHSEEVGHAKVFSKRNREQLEKIIKYSRSTEMSSAHARRILQQSNRNACIEAPETGSDLSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHLSENNEKGKCTKSLNSAAWIASNSELTPSESLASTDDETFGKNFSTEACQDCEQNDADNGSSMSTSSNFEPFATDDLGNTVIHLDQALARMREYERMKIEAESTLDSEGCSSNFQGASAAKLEGGQSASECVPVPQSSEASAVPCPHIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSISVKQRCKRKIEATEVIQSYAKEAKKGLQVDVCSSAEDVDEDKDKDETETVKQVQDSEMYDGNRVPESIRSDASDQEEDDESESGPVAISLSKAETQALTNYGSGEDENEDEEIEFEEGPVDVQTSLQASSETTTENEQNSNQEQSKTKSSEILSSEQESVYAKGEQHVATILPYYLNVMENAPPLTVNTPESFITDSMKTEESSSSLPVNETQTLDTTCVGNKSAASSESSMAGSPDTESPVLVNEYEAGSGNVSQRSDEDDFVKVEDLPLKLAVYSEADLMKKMAMEAQTNSLSDELLGGGGAQDQELVGDAQTLKEPETFGVQSA

Target ORF information:

RefSeq Version	XM_021285868.1
Organism	Columba livia(rock pigeon)
Definition	Columba livia pericentriolar material 1 (PCM1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021285868.1

ORF Insert Sequence:

ATGGCAACAG GAGGTGGTCC CTTTGAAGAA GGCATGAATG ATCAAGACTT GCCTAGCTGG 
AGCAATGACA GCTTTGATGA CCGGCTGAAC AACACGGACT GGGGAAGTCA ACAGAAGAAA 
GCAAACAGAT CTTCAGAAAA AAACAAGAAA AAGCTTGGTG GGGAAGCTGA AACAAGGCTT 
ACTAATGATA TATCTCCAGA ATCCTCACCT GGAATAGGAC GACGAAAGAC CAGAACTCCT 
CATAGTTTTC CTCATGCTCG ATACATGACC CAGATGTCTG TTCCGGAGCA GGCTGAGCTA 
GAAAGGCTTA AACAAAGAAT AAACTTCAGT GATCTGGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGCA GGGCAACAGC TGCTAACAAC AAACGTCAAC TTAATGAAAA CAAAAAACCA 
TTCAACTTCC TGTCACTGCA GATAAACACT AACAAGAGCA AAGATCCTGC CTCATGTTCC 
CAAAAAAAGG AAAGTGGGGT GTCAGTCCAA CGTAAAGAGT TGTTTGGTGC TGCTCTGAGC 
AAGGATTTCT TGCAAAATTG TCAAGTCTCT GCTCAAGAAG ATGGAGGGGG AGAACCAGCT 
ATGGATAGTA GCCAGGCTAG AGATCCTCAA CAGGAGGCTA AAGAGGAGTT GGAGAACTTG 
AAGAAGCAGC ATGATTTATT GAAAAGGATG CTAAAACAGC AAGAGCAATT AAAGGCTCTT 
CAAGGAAGAC AGGCAGCTCT TCTTGCTTTG CAGCATAAAG CAGAGCAAGC AATTGCTGTC 
ATGGATGATT CTGTTGTAAC AGAGACTACA GGTAGTGTTT CAGGAGTAAG CCTTACATCA 
GAACTGAATG AAGAATTGAA TGACTTAATT CAGCGCTTTC ACAACCAACT TCATGATTCT 
CAGACACAGT CTGTACCAGA CAATAGAAGG CAAGCAGAAA GTCTTTCACT TACTAGAGAG 
GTTTCACAAA GCAGAAACTC TTCAATGTCT GAACGCCTGT CAGATGAGAA GGCGCAGCTT 
TTTAACAAGA TGCGAATGTT GCAGGGTAAA AAGCAAAAAA TGGACAAACT GTTAGGAGAA 
CTTCATACAC TTCGTGACCA ACATCTAAAT AACTCCTCCT TTTTTCCTGC TTCAGGTTCT 
CCTCAAAGAA GTATTGATCA AAGAAGTACA ACTTCAGCTG CTTCTGGTCC TGCAGGCATA 
GTAACTGTTG TCAATGGCGA ATCAAATAGC CTGGCATCTG CTCCCTATCC TCCTGATTCC 
CTGGTTTCTC AGAATGAGAG TGAAGAAGAT GACAATCTAA ATCCAACAGA AAAGCTTCAG 
AAACTAAATG AAGTTCGTAA GAGGCTGAAT GAGTTACGTG AGCTAGTTCA CTACTATGAG 
CAAACATCTG ATATGATGAC AGATTCTGTG AATGAAAACA CTAAGGAAGA AGAAGAAACC 
GAAGAATCAG AAAGTGATTC TGAACGTGAG GATCCACAGC CTGTTACAAA TATTAGAAAT 
CCTCAAGGAA TCAGTAGTTG GAGTGACATA AATAGCAACT CAAATGTACA GTGTGGAACT 
AATAACAGAG ATGGAAGACA TCTTAATACA GACTGTGAAA TAAATAACAG ATCTGCTGCT 
AACATAAGGA CTCTAAAAAT GTCGTCACCT TTAGACTGTC ATAATAGGGA GGATGACAAA 
GATATTGGCC TACCCCAATG TGAAGATGAA GAAGTGGAAG AAGATAGAGT TAGTGAAGAT 
TCTGCATCTA GTCACAGAAG CAGCCTGGGT GATACTGCTG GAGATGCTGA GTTTGAGCAG 
AAGATCAATA GGCTTATAGC TGCAAAACAG AAACTTAGAC AATTACAAAA CCTTGCTGCT 
ATGGTGCAGG ATGATGATCC AGCACCTCAG ACAACAATTG CAAATGCATC TAATATTGGT 
GACTTGTTTT TGGGTGAGAT GGAAGAGACA AAGCAGCAGC CAAACAATGT CCGAGCTAGT 
ACCAACAAAT TACAAAAAGA TGTAGAACTG AATGAAAAAG CAAGAGAGAA GTTTTATGAA 
GCTAAACTCC AGCAGCAGCA ACAGGAGCTT AAGCAGTTAC AAGAAGAAAG AAGAAAACTG 
ATTGAAATTC AAGAAAAAAT TCAGGTGTTA CAGAAAGCTT GTCCTGATCT TCAATTGTCA 
GCTGGCTTGG GTGACTGCAC AGCAAATAGA CAGACTTCAC CAGCAACATC AACTCCAGCC 
ATGAATGACA CAGCTGGCAA GCCTTCACTT GAATTTGATG AATCTGTACC AGTAGGCAAT 
GAGTTATGGT CTGAGATGAG AAGACATGAG ATTTTAAGAG AAGAATTGCG ACAAAGAAGA 
AAGCAACTGG AAGCTCTAAT GGCTGAGCAT CAGAGGAGGA AAGAGCTCGC AGAAACAATA 
TCTACTGTTG CTGCGTCTGT TAAAAGTGAA GGATCAGAAG CTCGGCGTAC TCCACAGCAG 
AGTAGGACTG AAAATAGGAC AATGGCTACC TGGGGAGGTT CTACGCAGTG TGCACTAGAT 
GAAGAGGATG GAGATGAAGA TGGTTATCTC TCTGATGGAG CTGGTCAGGC AGAAGAAGAT 
GATGATGATG CGTCAAGTGC GAATGACAGC TTCTCTGTTT ATCCCAATAA CAACGTACCA 
GAAAATGCAT ATTTTGTTAA AGAAAACAAG GATAGGTGGA AAAATTACCG TCCTCTTTCA 
GCAGATGGAA ATTATCGTCC ATTGTCTAAG GCCAGACAAC AGCAAAACAT AAGTATGCGA 
CGTCAGGAAA ACCTTCGGTG GATGTCTGAA CTTTCATATG TGGAAGAAAA GGAACGATGG 
CAAGAGCAGA TCAATCAGTT GAAGAAACAG CATGAATTTA GTGTCAGCAT TTGTCAAACT 
TTAATGCAGG ATCAGCAGAC CCTCTCTTGT CTTCTACAGA CTTTGCTTAC AGGCCCTTAC 
AGTATGATGC CCAATAATGT TGCATCTTCA CAAGTACATC TTATTATGCA TCAGCTAAAC 
CAGTGTTACA CTCAACTGAC ATGGCAGCAG AATAACGTCC AGAGGTTGAA ACAAATGTTA 
AATGATCTTG TGCGTCAGCA AGAAGAACAA CGGTATGAAG AGAAGTCATC AAGAAAGGAT 
AGAGGCAGTG GTGTACCCCC ACCTCCATCT CCTGTTTTCT GTCCGTTCAG CTTTCCTCCA 
CAACCTTTGA ACCTGTTTAA TGTACCAGGA TTTACTAATT TTTCTTCCTT TGCTCCAGAT 
ATTAACTATA ATCCAGTGTT TCCTTCTGGT TTTGGAGATT TTGCACACAA TATTTCTCCA 
CACAGCAGTG AGCAGCAGCA GCAACATCCT CTAGATCATA ATGCATCTGG GAAAACTGAG 
TATATGGCGT TCCCCAAACC TTTTGAAAGC AGTTCCTCTA ATGGAGCGGA AAAACAAAGG 
AGGAGTCACA GACAACCTGA AGAGGAAATG GAAAAAAGAT CAGCTTGGCT TAATGATAGC 
CAGGACGTGA AAAAAGATGA TCAGTCTCAG CTGAAAGCAA GTTTTGCTGT TTCAGTACAA 
AACATTGCTT CCAATCATAA AAATCAGTCT GATATGAGCA GAAGAAGAGA GTTTGATGAA 
GAGTCTTTGG AGAGTTTTAG TAGCATGCCT GATCCAGTAG ACCCCACTAC TGTGACAAAG 
ACATTTAAAT CTAGAAAAGC ATCAGCACAA GCAAGTTTGG CATCAAAAGA TAAAACACCT 
AAATCGAAGA ATAAGAGGAA GAGTTCTTCT CAGCTAAAAG GCAGAATTAC AAATATTGAT 
TATGAAAGTG CCAGTGCTTC TAGTGTGTGT GAACCCTGCA AGAGCAATAA AAGCAGACAC 
TCTGAAGAGG TTGGTCATGC AAAGGTGTTC AGCAAAAGGA ATCGGGAACA ACTGGAAAAA 
ATCATTAAAT ACAGTAGATC TACAGAAATG TCTTCAGCGC ATGCTAGGAG AATTCTGCAG 
CAGTCTAACA GAAATGCATG CATTGAAGCG CCAGAAACTG GTAGTGATCT TTCTATGTTT 
GAAGCTTTAC GAGACACAAT TTATTCTGAA GTGGCAACTC TTATTTCTCA AAATGAGTCT 
CGTCCCCACT TTCTTATTGA ACTTTTCCAT GAGCTTCAGC TGCTAAATAC AGATTACCTG 
AGGCAAAGAG CTCTATACGC TCTACAGGAT ATAGTGACCA GACATTTATC TGAGAACAAT 
GAAAAAGGGA AGTGCACAAA ATCACTGAAT TCTGCAGCAT GGATAGCATC AAATTCTGAA 
CTCACTCCCA GTGAAAGCCT TGCTTCTACA GATGATGAAA CTTTTGGCAA AAACTTTTCT 
ACAGAAGCAT GTCAAGATTG TGAACAAAAT GATGCAGACA ATGGGAGTAG CATGTCTACA 
TCTTCAAATT TTGAACCCTT TGCCACTGAT GACCTTGGCA ACACAGTGAT TCACTTAGAT 
CAAGCTTTGG CTAGGATGAG GGAGTATGAG CGTATGAAAA TTGAAGCTGA AAGTACCCTT 
GACTCTGAGG GCTGCTCTAG TAATTTTCAG GGTGCTTCTG CTGCTAAATT AGAAGGAGGT 
CAAAGTGCCA GTGAATGTGT TCCTGTGCCA CAGTCAAGTG AAGCTTCTGC TGTTCCATGT 
CCTCATATAG ACACTCAGCA GCTTGACCGG CAGATTAAAG CAATTATGAA AGAGGTCATT 
CCTTTTCTGA AGGAACACAT GGATGAAGTA TGCTCTTCGC AGTTACTGAC ATCAGTAAGA 
CGTATGGTCT TGACCCTTAC ACAACAAAAT GATGAAAGTA AAGAGTTTGT GAAGTTCTTT 
CATAAGCAGC TTGGCAGTAT ACTTCAGGAT TCACTGGCAA AATTTGCTGG TAGAAAACTG 
AAAGATTGTG GAGAGGATCT TCTAGTGGAG ATCTCTGAAG TGTTATTTAA TGAATTAGCC 
TTTTTTAAAC TCATGCAAGA CTTGGACAAC AACAGTATTT CTGTAAAGCA GAGATGTAAA 
AGAAAAATAG AAGCTACTGA AGTGATACAG TCGTATGCTA AAGAGGCAAA AAAAGGTCTC 
CAGGTGGATG TTTGTTCATC TGCTGAAGAT GTCGATGAGG ACAAAGACAA GGATGAGACT 
GAAACTGTTA AACAAGTACA GGACTCAGAA ATGTATGATG GGAATAGAGT TCCTGAAAGT 
ATTAGGTCTG ATGCATCTGA TCAGGAGGAA GATGATGAAA GTGAAAGCGG TCCAGTGGCA 
ATAAGTTTAT CAAAAGCAGA AACCCAAGCT CTGACTAACT ATGGTAGTGG AGAAGATGAG 
AATGAAGATG AAGAAATAGA ATTTGAGGAA GGACCTGTTG ATGTGCAGAC GTCATTACAA 
GCCAGCAGTG AAACAACAAC TGAAAATGAA CAGAATTCAA ACCAAGAACA AAGTAAGACA 
AAAAGCAGTG AGATTCTGTC ATCAGAACAA GAATCTGTTT ATGCTAAAGG TGAACAACAT 
GTGGCTACAA TTTTGCCTTA TTACCTCAAT GTAATGGAGA ATGCACCGCC TTTAACGGTC 
AATACCCCAG AATCTTTTAT AACAGACAGT ATGAAAACAG AAGAATCAAG TTCATCTTTA 
CCAGTAAATG AAACTCAAAC ACTGGATACA ACGTGTGTAG GAAACAAATC TGCTGCAAGT 
TCTGAAAGCT CCATGGCTGG CAGCCCTGAT ACAGAGTCAC CTGTGTTAGT GAATGAATAT 
GAAGCTGGTT CTGGAAATGT AAGTCAAAGA TCTGATGAAG ATGACTTTGT GAAAGTTGAA 
GACTTGCCCC TTAAACTTGC AGTGTATTCA GAGGCAGATT TAATGAAGAA AATGGCAATG 
GAGGCCCAAA CGAACAGTTT GTCTGATGAA TTACTGGGTG GAGGTGGAGC TCAAGATCAA 
GAATTAGTAG GAGATGCCCA AACTTTGAAA GAACCTGAAA CTTTTGGAGT TCAAAGTGCG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_021285869.1 ) cDNA ORF clone, Columba livia(rock pigeon) -> XP_021141544.1 Columba livia pericentriolar material 1 (PCM1), transcript variant X16, mRNA.

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CloneID	OCo293172
Clone ID Related Accession (Same CDS sequence)	XM_021285869.1
Accession Version	XM_021285869.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5826bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-23
Organism	Columba livia(rock pigeon)
Product	pericentriolar material 1 protein isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004973253.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Columba livia Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACAG GAGGTGGTCC CTTTGAAGAA GGCATGAATG ATCAAGACTT GCCTAGCTGG 
AGCAATGACA GCTTTGATGA CCGGCTGAAC AACACGGACT GGGGAAGTCA ACAGAAGAAA 
GCAAACAGAT CTTCAGAAAA AAACAAGAAA AAGCTTGGTG GGGAAGCTGA AACAAGGCTT 
ACTAATGATA TATCTCCAGA ATCCTCACCT GGAATAGGAC GACGAAAGAC CAGAACTCCT 
CATAGTTTTC CTCATGCTCG ATACATGACC CAGATGTCTG TTCCGGAGCA GGCTGAGCTA 
GAAAGGCTTA AACAAAGAAT AAACTTCAGT GATCTGGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGCA GGGCAACAGC TGCTAACAAC AAACGTCAAC TTAATGAAAA CAAAAAACCA 
TTCAACTTCC TGTCACTGCA GATAAACACT AACAAGAGCA AAGATCCTGC CTCATGTTCC 
CAAAAAAAGG AAAGTGGGGT GTCAGTCCAA CGTAAAGAGT TGTTTGGTGC TGCTCTGAGC 
AAGGATTTCT TGCAAAATTG TCAAGTCTCT GCTCAAGAAG ATGGAGGGGG AGAACCAGCT 
ATGGATAGTA GCCAGGCTAG AGATCCTCAA CAGGAGGCTA AAGAGGAGTT GGAGAACTTG 
AAGAAGCAGC ATGATTTATT GAAAAGGATG CTAAAACAGC AAGAGCAATT AAAGGCTCTT 
CAAGGAAGAC AGGCAGCTCT TCTTGCTTTG CAGCATAAAG CAGAGCAAGC AATTGCTGTC 
ATGGATGATT CTGTTGTAAC AGAGACTACA GGTAGTGTTT CAGGAGTAAG CCTTACATCA 
GAACTGAATG AAGAATTGAA TGACTTAATT CAGCGCTTTC ACAACCAACT TCATGATTCT 
CAGACACAGT CTGTACCAGA CAATAGAAGG CAAGCAGAAA GTCTTTCACT TACTAGAGAG 
GTTTCACAAA GCAGAAACTC TTCAATGTCT GAACGCCTGT CAGATGAGAA GGCGCAGCTT 
TTTAACAAGA TGCGAATGTT GCAGGGTAAA AAGCAAAAAA TGGACAAACT GTTAGGAGAA 
CTTCATACAC TTCGTGACCA ACATCTAAAT AACTCCTCCT TTTTTCCTGC TTCAGGTTCT 
CCTCAAAGAA GTATTGATCA AAGAAGTACA ACTTCAGCTG CTTCTGGTCC TGCAGGCATA 
GTAACTGTTG TCAATGGCGA ATCAAATAGC CTGGCATCTG CTCCCTATCC TCCTGATTCC 
CTGGTTTCTC AGAATGAGAG TGAAGAAGAT GACAATCTAA ATCCAACAGA AAAGCTTCAG 
AAACTAAATG AAGTTCGTAA GAGGCTGAAT GAGTTACGTG AGCTAGTTCA CTACTATGAG 
CAAACATCTG ATATGATGAC AGATTCTGTG AATGAAAACA CTAAGGAAGA AGAAGAAACC 
GAAGAATCAG AAAGTGATTC TGAACGTGAG GATCCACAGC CTGTTACAAA TATTAGAAAT 
CCTCAAGGAA TCAGTAGTTG GAGTGACATA AATAGCAACT CAAATGTACA GTGTGGAACT 
AATAACAGAG ATGGAAGACA TCTTAATACA GACTGTGAAA TAAATAACAG ATCTGCTGCT 
AACATAAGGA CTCTAAAAAT GTCGTCACCT TTAGACTGTC ATAATAGGGA GGATGACAAA 
GATATTGGCC TACCCCAATG TGAAGATGAA GAAGTGGAAG AAGATAGAGT TAGTGAAGAT 
TCTGCATCTA GTCACAGAAG CAGCCTGGGT GATACTGCTG GAGATGCTGA GTTTGAGCAG 
AAGATCAATA GGCTTATAGC TGCAAAACAG AAACTTAGAC AATTACAAAA CCTTGCTGCT 
ATGGTGCAGG ATGATGATCC AGCACCTCAG ACAACAATTG CAAATGCATC TAATATTGGT 
GACTTGTTTT TGGGTGAGAT GGAAGAGACA AAGCAGCAGC CAAACAATGT CCGAGCTAGT 
ACCAACAAAT TACAAAAAGA TGTAGAACTG AATGAAAAAG CAAGAGAGAA GTTTTATGAA 
GCTAAACTCC AGCAGCAGCA ACAGGAGCTT AAGCAGTTAC AAGAAGAAAG AAGAAAACTG 
ATTGAAATTC AAGAAAAAAT TCAGGTGTTA CAGAAAGCTT GTCCTGATCT TCAATTGTCA 
GCTGGCTTGG GTGACTGCAC AGCAAATAGA CAGACTTCAC CAGCAACATC AACTCCAGCC 
ATGAATGACA CAGCTGGCAA GCCTTCACTT GAATTTGATG AATCTGTACC AGTAGGCAAT 
GAGTTATGGT CTGAGATGAG AAGACATGAG ATTTTAAGAG AAGAATTGCG ACAAAGAAGA 
AAGCAACTGG AAGCTCTAAT GGCTGAGCAT CAGAGGAGGA AAGAGCTCGC AGAAACAATA 
TCTACTGTTG CTGCGTCTGT TAAAAGTGAA GGATCAGAAG CTCGGCGTAC TCCACAGCAG 
AGTAGGACTG AAAATAGGAC AATGGCTACC TGGGGAGGTT CTACGCAGTG TGCACTAGAT 
GAAGAGGATG GAGATGAAGA TGGTTATCTC TCTGATGGAG CTGGTCAGGC AGAAGAAGAT 
GATGATGATG CGTCAAGTGC GAATGACAGC TTCTCTGTTT ATCCCAATAA CAACGTACCA 
GAAAATGCAT ATTTTGTTAA AGAAAACAAG GATAGGTGGA AAAATTACCG TCCTCTTTCA 
GCAGATGGAA ATTATCGTCC ATTGTCTAAG GCCAGACAAC AGCAAAACAT AAGTATGCGA 
CGTCAGGAAA ACCTTCGGTG GATGTCTGAA CTTTCATATG TGGAAGAAAA GGAACGATGG 
CAAGAGCAGA TCAATCAGTT GAAGAAACAG CATGAATTTA GTGTCAGCAT TTGTCAAACT 
TTAATGCAGG ATCAGCAGAC CCTCTCTTGT CTTCTACAGA CTTTGCTTAC AGGCCCTTAC 
AGTATGATGC CCAATAATGT TGCATCTTCA CAAGTACATC TTATTATGCA TCAGCTAAAC 
CAGTGTTACA CTCAACTGAC ATGGCAGCAG AATAACGTCC AGAGGTTGAA ACAAATGTTA 
AATGATCTTG TGCGTCAGCA AGAAGAACAA CGGTATGAAG AGAAGTCATC AAGAAAGGAT 
AGAGGCAGTG GTGTACCCCC ACCTCCATCT CCTGTTTTCT GTCCGTTCAG CTTTCCTCCA 
CAACCTTTGA ACCTGTTTAA TGTACCAGGA TTTACTAATT TTTCTTCCTT TGCTCCAGAT 
ATTAACTATA ATCCAGTGTT TCCTTCTGGT TTTGGAGATT TTGCACACAA TATTTCTCCA 
CACAGCAGTG AGCAGCAGCA GCAACATCCT CTAGATCATA ATGCATCTGG GAAAACTGAG 
TATATGGCGT TCCCCAAACC TTTTGAAAGC AGTTCCTCTA ATGGAGCGGA AAAACAAAGG 
AGGAGTCACA GACAACCTGA AGAGGAAATG GAAAAAAGAT CAGCTTGGCT TAATGATAGC 
CAGGACGTGA AAAAAGATGA TCAGTCTCAG CTGAAAGCAA GTTTTGCTGT TTCAGTACAA 
AACATTGCTT CCAATCATAA AAATCAGTCT GATATGAGCA GAAGAAGAGA GTTTGATGAA 
GAGTCTTTGG AGAGTTTTAG TAGCATGCCT GATCCAGTAG ACCCCACTAC TGTGACAAAG 
ACATTTAAAT CTAGAAAAGC ATCAGCACAA GCAAGTTTGG CATCAAAAGA TAAAACACCT 
AAATCGAAGA ATAAGAGGAA GAGTTCTTCT CAGCTAAAAG GCAGAATTAC AAATATTGAT 
TATGAAAGTG CCAGTGCTTC TAGTGTGTGT GAACCCTGCA AGAGCAATAA AAGCAGACAC 
TCTGAAGAGG TTGGTCATGC AAAGGTGTTC AGCAAAAGGA ATCGGGAACA ACTGGAAAAA 
ATCATTAAAT ACAGTAGATC TACAGAAATG TCTTCAGAAA CTGGTAGTGA TCTTTCTATG 
TTTGAAGCTT TACGAGACAC AATTTATTCT GAAGTGGCAA CTCTTATTTC TCAAAATGAG 
TCTCGTCCCC ACTTTCTTAT TGAACTTTTC CATGAGCTTC AGCTGCTAAA TACAGATTAC 
CTGAGGCAAA GAGCTCTATA CGCTCTACAG GATATAGTGA CCAGACATTT ATCTGAGAAC 
AATGAAAAAG GGAAGTGCAC AAAATCACTG AATTCTGCAG CATGGATAGC ATCAAATTCT 
GAACTCACTC CCAGTGAAAG CCTTGCTTCT ACAGATGATG AAACTTTTGG CAAAAACTTT 
TCTACAGAAG CATGTCAAGA TTGTGAACAA AATGATGCAG ACAATGGGAG TAGCATGTCT 
ACATCTTCAA ATTTTGAACC CTTTGCCACT GATGACCTTG GCAACACAGT GATTCACTTA 
GATCAAGCTT TGGCTAGGAT GAGGGAGTAT GAGCGTATGA AAATTGAAGC TGAAAGTACC 
CTTGACTCTG AGGGCTGCTC TAGTAATTTT CAGGGTGCTT CTGCTGCTAA ATTAGAAGGA 
GGTCAAAGTG CCAGTGAATG TGTTCCTGTG CCACAGTCAA GTGAAGCTTC TGCTGTTCCA 
TGTCCTCATA TAGACACTCA GCAGCTTGAC CGGCAGATTA AAGCAATTAT GAAAGAGGTC 
ATTCCTTTTC TGAAGGAACA CATGGATGAA GTATGCTCTT CGCAGTTACT GACATCAGTA 
AGACGTATGG TCTTGACCCT TACACAACAA AATGATGAAA GTAAAGAGTT TGTGAAGTTC 
TTTCATAAGC AGCTTGGCAG TATACTTCAG GATTCACTGG CAAAATTTGC TGGTAGAAAA 
CTGAAAGATT GTGGAGAGGA TCTTCTAGTG GAGATCTCTG AAGTGTTATT TAATGAATTA 
GCCTTTTTTA AACTCATGCA AGACTTGGAC AACAACAGTA TTTCTGTAAA GCAGAGATGT 
AAAAGAAAAA TAGAAGCTAC TGAAGTGATA CAGTCGTATG CTAAAGAGGA CAAGGATGAG 
ACTGAAACTG TTAAACAAGT ACAGGACTCA GAAATGTATG ATGGGAATAG AGTTCCTGAA 
AGTATTAGGT CTGATGCATC TGATCAGGAG GAAGATGATG AAAGTGAAAG CGGTCCAGTG 
GCAATAAGTT TATCAAAAGC AGAAACCCAA GCTCTGACTA ACTATGGTAG TGGAGAAGAT 
GAGAATGAAG ATGAAGAAAT AGAATTTGAG GAAGGACCTG TTGATGTGCA GACGTCATTA 
CAAGCCAGCA GTGAAACAAC AACTGAAAAT GAACAGAATT CAAACCAAGA ACAAAGTAAG 
ACAAAAAGCA GTGAGATTCT GTCATCAGAA CAAGAATCTG TTTATGCTAA AGGTGAACAA 
CATGTGGCTA CAATTTTGCC TTATTACCTC AATGTAATGG AGAATGCACC GCCTTTAACG 
GTCAATACCC CAGAATCTTT TATAACAGAC AGTATGAAAA CAGAAGAATC AAGTTCATCT 
TTACCAGTAA ATGAAACTCA AACACTGGAT ACAACGTGTG TAGGAAACAA ATCTGCTGCA 
AGTTCTGAAA GCTCCATGGC TGGCAGCCCT GATACAGAGT CACCTGTGTT AGTGAATGAA 
TATGAAGCTG GTTCTGGAAA TGTAAGTCAA AGATCTGATG AAGATGACTT TGTGAAAGTT 
GAAGACTTGC CCCTTAAACT TGCAGTGTAT TCAGAGGCAG ATTTAATGAA GAAAATGGCA 
ATGGAGGCCC AAACGAACAG TTTGTCTGAT GAATTACTGG GTGGAGGTGG AGCTCAAGAT 
CAAGAATTAG TAGGAGATGC CCAAACTTTG AAAGAACCTG AAACTTTTGG AGTTCAAAGT 
GCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021141544.1
CDS	140..5965
Translation	MATGGGPFEEGMNDQDLPSWSNDSFDDRLNNTDWGSQQKKANRSSEKNKKKLGGEAETRLTNDISPESSPGIGRRKTRTPHSFPHARYMTQMSVPEQAELERLKQRINFSDLDQRSIGSDSQGRATAANNKRQLNENKKPFNFLSLQINTNKSKDPASCSQKKESGVSVQRKELFGAALSKDFLQNCQVSAQEDGGGEPAMDSSQARDPQQEAKEELENLKKQHDLLKRMLKQQEQLKALQGRQAALLALQHKAEQAIAVMDDSVVTETTGSVSGVSLTSELNEELNDLIQRFHNQLHDSQTQSVPDNRRQAESLSLTREVSQSRNSSMSERLSDEKAQLFNKMRMLQGKKQKMDKLLGELHTLRDQHLNNSSFFPASGSPQRSIDQRSTTSAASGPAGIVTVVNGESNSLASAPYPPDSLVSQNESEEDDNLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDSVNENTKEEEETEESESDSEREDPQPVTNIRNPQGISSWSDINSNSNVQCGTNNRDGRHLNTDCEINNRSAANIRTLKMSSPLDCHNREDDKDIGLPQCEDEEVEEDRVSEDSASSHRSSLGDTAGDAEFEQKINRLIAAKQKLRQLQNLAAMVQDDDPAPQTTIANASNIGDLFLGEMEETKQQPNNVRASTNKLQKDVELNEKAREKFYEAKLQQQQQELKQLQEERRKLIEIQEKIQVLQKACPDLQLSAGLGDCTANRQTSPATSTPAMNDTAGKPSLEFDESVPVGNELWSEMRRHEILREELRQRRKQLEALMAEHQRRKELAETISTVAASVKSEGSEARRTPQQSRTENRTMATWGGSTQCALDEEDGDEDGYLSDGAGQAEEDDDDASSANDSFSVYPNNNVPENAYFVKENKDRWKNYRPLSADGNYRPLSKARQQQNISMRRQENLRWMSELSYVEEKERWQEQINQLKKQHEFSVSICQTLMQDQQTLSCLLQTLLTGPYSMMPNNVASSQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNDLVRQQEEQRYEEKSSRKDRGSGVPPPPSPVFCPFSFPPQPLNLFNVPGFTNFSSFAPDINYNPVFPSGFGDFAHNISPHSSEQQQQHPLDHNASGKTEYMAFPKPFESSSSNGAEKQRRSHRQPEEEMEKRSAWLNDSQDVKKDDQSQLKASFAVSVQNIASNHKNQSDMSRRREFDEESLESFSSMPDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKNKRKSSSQLKGRITNIDYESASASSVCEPCKSNKSRHSEEVGHAKVFSKRNREQLEKIIKYSRSTEMSSETGSDLSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHLSENNEKGKCTKSLNSAAWIASNSELTPSESLASTDDETFGKNFSTEACQDCEQNDADNGSSMSTSSNFEPFATDDLGNTVIHLDQALARMREYERMKIEAESTLDSEGCSSNFQGASAAKLEGGQSASECVPVPQSSEASAVPCPHIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSISVKQRCKRKIEATEVIQSYAKEDKDETETVKQVQDSEMYDGNRVPESIRSDASDQEEDDESESGPVAISLSKAETQALTNYGSGEDENEDEEIEFEEGPVDVQTSLQASSETTTENEQNSNQEQSKTKSSEILSSEQESVYAKGEQHVATILPYYLNVMENAPPLTVNTPESFITDSMKTEESSSSLPVNETQTLDTTCVGNKSAASSESSMAGSPDTESPVLVNEYEAGSGNVSQRSDEDDFVKVEDLPLKLAVYSEADLMKKMAMEAQTNSLSDELLGGGGAQDQELVGDAQTLKEPETFGVQSA

Target ORF information:

RefSeq Version	XM_021285869.1
Organism	Columba livia(rock pigeon)
Definition	Columba livia pericentriolar material 1 (PCM1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021285869.1

ORF Insert Sequence:

ATGGCAACAG GAGGTGGTCC CTTTGAAGAA GGCATGAATG ATCAAGACTT GCCTAGCTGG 
AGCAATGACA GCTTTGATGA CCGGCTGAAC AACACGGACT GGGGAAGTCA ACAGAAGAAA 
GCAAACAGAT CTTCAGAAAA AAACAAGAAA AAGCTTGGTG GGGAAGCTGA AACAAGGCTT 
ACTAATGATA TATCTCCAGA ATCCTCACCT GGAATAGGAC GACGAAAGAC CAGAACTCCT 
CATAGTTTTC CTCATGCTCG ATACATGACC CAGATGTCTG TTCCGGAGCA GGCTGAGCTA 
GAAAGGCTTA AACAAAGAAT AAACTTCAGT GATCTGGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGCA GGGCAACAGC TGCTAACAAC AAACGTCAAC TTAATGAAAA CAAAAAACCA 
TTCAACTTCC TGTCACTGCA GATAAACACT AACAAGAGCA AAGATCCTGC CTCATGTTCC 
CAAAAAAAGG AAAGTGGGGT GTCAGTCCAA CGTAAAGAGT TGTTTGGTGC TGCTCTGAGC 
AAGGATTTCT TGCAAAATTG TCAAGTCTCT GCTCAAGAAG ATGGAGGGGG AGAACCAGCT 
ATGGATAGTA GCCAGGCTAG AGATCCTCAA CAGGAGGCTA AAGAGGAGTT GGAGAACTTG 
AAGAAGCAGC ATGATTTATT GAAAAGGATG CTAAAACAGC AAGAGCAATT AAAGGCTCTT 
CAAGGAAGAC AGGCAGCTCT TCTTGCTTTG CAGCATAAAG CAGAGCAAGC AATTGCTGTC 
ATGGATGATT CTGTTGTAAC AGAGACTACA GGTAGTGTTT CAGGAGTAAG CCTTACATCA 
GAACTGAATG AAGAATTGAA TGACTTAATT CAGCGCTTTC ACAACCAACT TCATGATTCT 
CAGACACAGT CTGTACCAGA CAATAGAAGG CAAGCAGAAA GTCTTTCACT TACTAGAGAG 
GTTTCACAAA GCAGAAACTC TTCAATGTCT GAACGCCTGT CAGATGAGAA GGCGCAGCTT 
TTTAACAAGA TGCGAATGTT GCAGGGTAAA AAGCAAAAAA TGGACAAACT GTTAGGAGAA 
CTTCATACAC TTCGTGACCA ACATCTAAAT AACTCCTCCT TTTTTCCTGC TTCAGGTTCT 
CCTCAAAGAA GTATTGATCA AAGAAGTACA ACTTCAGCTG CTTCTGGTCC TGCAGGCATA 
GTAACTGTTG TCAATGGCGA ATCAAATAGC CTGGCATCTG CTCCCTATCC TCCTGATTCC 
CTGGTTTCTC AGAATGAGAG TGAAGAAGAT GACAATCTAA ATCCAACAGA AAAGCTTCAG 
AAACTAAATG AAGTTCGTAA GAGGCTGAAT GAGTTACGTG AGCTAGTTCA CTACTATGAG 
CAAACATCTG ATATGATGAC AGATTCTGTG AATGAAAACA CTAAGGAAGA AGAAGAAACC 
GAAGAATCAG AAAGTGATTC TGAACGTGAG GATCCACAGC CTGTTACAAA TATTAGAAAT 
CCTCAAGGAA TCAGTAGTTG GAGTGACATA AATAGCAACT CAAATGTACA GTGTGGAACT 
AATAACAGAG ATGGAAGACA TCTTAATACA GACTGTGAAA TAAATAACAG ATCTGCTGCT 
AACATAAGGA CTCTAAAAAT GTCGTCACCT TTAGACTGTC ATAATAGGGA GGATGACAAA 
GATATTGGCC TACCCCAATG TGAAGATGAA GAAGTGGAAG AAGATAGAGT TAGTGAAGAT 
TCTGCATCTA GTCACAGAAG CAGCCTGGGT GATACTGCTG GAGATGCTGA GTTTGAGCAG 
AAGATCAATA GGCTTATAGC TGCAAAACAG AAACTTAGAC AATTACAAAA CCTTGCTGCT 
ATGGTGCAGG ATGATGATCC AGCACCTCAG ACAACAATTG CAAATGCATC TAATATTGGT 
GACTTGTTTT TGGGTGAGAT GGAAGAGACA AAGCAGCAGC CAAACAATGT CCGAGCTAGT 
ACCAACAAAT TACAAAAAGA TGTAGAACTG AATGAAAAAG CAAGAGAGAA GTTTTATGAA 
GCTAAACTCC AGCAGCAGCA ACAGGAGCTT AAGCAGTTAC AAGAAGAAAG AAGAAAACTG 
ATTGAAATTC AAGAAAAAAT TCAGGTGTTA CAGAAAGCTT GTCCTGATCT TCAATTGTCA 
GCTGGCTTGG GTGACTGCAC AGCAAATAGA CAGACTTCAC CAGCAACATC AACTCCAGCC 
ATGAATGACA CAGCTGGCAA GCCTTCACTT GAATTTGATG AATCTGTACC AGTAGGCAAT 
GAGTTATGGT CTGAGATGAG AAGACATGAG ATTTTAAGAG AAGAATTGCG ACAAAGAAGA 
AAGCAACTGG AAGCTCTAAT GGCTGAGCAT CAGAGGAGGA AAGAGCTCGC AGAAACAATA 
TCTACTGTTG CTGCGTCTGT TAAAAGTGAA GGATCAGAAG CTCGGCGTAC TCCACAGCAG 
AGTAGGACTG AAAATAGGAC AATGGCTACC TGGGGAGGTT CTACGCAGTG TGCACTAGAT 
GAAGAGGATG GAGATGAAGA TGGTTATCTC TCTGATGGAG CTGGTCAGGC AGAAGAAGAT 
GATGATGATG CGTCAAGTGC GAATGACAGC TTCTCTGTTT ATCCCAATAA CAACGTACCA 
GAAAATGCAT ATTTTGTTAA AGAAAACAAG GATAGGTGGA AAAATTACCG TCCTCTTTCA 
GCAGATGGAA ATTATCGTCC ATTGTCTAAG GCCAGACAAC AGCAAAACAT AAGTATGCGA 
CGTCAGGAAA ACCTTCGGTG GATGTCTGAA CTTTCATATG TGGAAGAAAA GGAACGATGG 
CAAGAGCAGA TCAATCAGTT GAAGAAACAG CATGAATTTA GTGTCAGCAT TTGTCAAACT 
TTAATGCAGG ATCAGCAGAC CCTCTCTTGT CTTCTACAGA CTTTGCTTAC AGGCCCTTAC 
AGTATGATGC CCAATAATGT TGCATCTTCA CAAGTACATC TTATTATGCA TCAGCTAAAC 
CAGTGTTACA CTCAACTGAC ATGGCAGCAG AATAACGTCC AGAGGTTGAA ACAAATGTTA 
AATGATCTTG TGCGTCAGCA AGAAGAACAA CGGTATGAAG AGAAGTCATC AAGAAAGGAT 
AGAGGCAGTG GTGTACCCCC ACCTCCATCT CCTGTTTTCT GTCCGTTCAG CTTTCCTCCA 
CAACCTTTGA ACCTGTTTAA TGTACCAGGA TTTACTAATT TTTCTTCCTT TGCTCCAGAT 
ATTAACTATA ATCCAGTGTT TCCTTCTGGT TTTGGAGATT TTGCACACAA TATTTCTCCA 
CACAGCAGTG AGCAGCAGCA GCAACATCCT CTAGATCATA ATGCATCTGG GAAAACTGAG 
TATATGGCGT TCCCCAAACC TTTTGAAAGC AGTTCCTCTA ATGGAGCGGA AAAACAAAGG 
AGGAGTCACA GACAACCTGA AGAGGAAATG GAAAAAAGAT CAGCTTGGCT TAATGATAGC 
CAGGACGTGA AAAAAGATGA TCAGTCTCAG CTGAAAGCAA GTTTTGCTGT TTCAGTACAA 
AACATTGCTT CCAATCATAA AAATCAGTCT GATATGAGCA GAAGAAGAGA GTTTGATGAA 
GAGTCTTTGG AGAGTTTTAG TAGCATGCCT GATCCAGTAG ACCCCACTAC TGTGACAAAG 
ACATTTAAAT CTAGAAAAGC ATCAGCACAA GCAAGTTTGG CATCAAAAGA TAAAACACCT 
AAATCGAAGA ATAAGAGGAA GAGTTCTTCT CAGCTAAAAG GCAGAATTAC AAATATTGAT 
TATGAAAGTG CCAGTGCTTC TAGTGTGTGT GAACCCTGCA AGAGCAATAA AAGCAGACAC 
TCTGAAGAGG TTGGTCATGC AAAGGTGTTC AGCAAAAGGA ATCGGGAACA ACTGGAAAAA 
ATCATTAAAT ACAGTAGATC TACAGAAATG TCTTCAGAAA CTGGTAGTGA TCTTTCTATG 
TTTGAAGCTT TACGAGACAC AATTTATTCT GAAGTGGCAA CTCTTATTTC TCAAAATGAG 
TCTCGTCCCC ACTTTCTTAT TGAACTTTTC CATGAGCTTC AGCTGCTAAA TACAGATTAC 
CTGAGGCAAA GAGCTCTATA CGCTCTACAG GATATAGTGA CCAGACATTT ATCTGAGAAC 
AATGAAAAAG GGAAGTGCAC AAAATCACTG AATTCTGCAG CATGGATAGC ATCAAATTCT 
GAACTCACTC CCAGTGAAAG CCTTGCTTCT ACAGATGATG AAACTTTTGG CAAAAACTTT 
TCTACAGAAG CATGTCAAGA TTGTGAACAA AATGATGCAG ACAATGGGAG TAGCATGTCT 
ACATCTTCAA ATTTTGAACC CTTTGCCACT GATGACCTTG GCAACACAGT GATTCACTTA 
GATCAAGCTT TGGCTAGGAT GAGGGAGTAT GAGCGTATGA AAATTGAAGC TGAAAGTACC 
CTTGACTCTG AGGGCTGCTC TAGTAATTTT CAGGGTGCTT CTGCTGCTAA ATTAGAAGGA 
GGTCAAAGTG CCAGTGAATG TGTTCCTGTG CCACAGTCAA GTGAAGCTTC TGCTGTTCCA 
TGTCCTCATA TAGACACTCA GCAGCTTGAC CGGCAGATTA AAGCAATTAT GAAAGAGGTC 
ATTCCTTTTC TGAAGGAACA CATGGATGAA GTATGCTCTT CGCAGTTACT GACATCAGTA 
AGACGTATGG TCTTGACCCT TACACAACAA AATGATGAAA GTAAAGAGTT TGTGAAGTTC 
TTTCATAAGC AGCTTGGCAG TATACTTCAG GATTCACTGG CAAAATTTGC TGGTAGAAAA 
CTGAAAGATT GTGGAGAGGA TCTTCTAGTG GAGATCTCTG AAGTGTTATT TAATGAATTA 
GCCTTTTTTA AACTCATGCA AGACTTGGAC AACAACAGTA TTTCTGTAAA GCAGAGATGT 
AAAAGAAAAA TAGAAGCTAC TGAAGTGATA CAGTCGTATG CTAAAGAGGA CAAGGATGAG 
ACTGAAACTG TTAAACAAGT ACAGGACTCA GAAATGTATG ATGGGAATAG AGTTCCTGAA 
AGTATTAGGT CTGATGCATC TGATCAGGAG GAAGATGATG AAAGTGAAAG CGGTCCAGTG 
GCAATAAGTT TATCAAAAGC AGAAACCCAA GCTCTGACTA ACTATGGTAG TGGAGAAGAT 
GAGAATGAAG ATGAAGAAAT AGAATTTGAG GAAGGACCTG TTGATGTGCA GACGTCATTA 
CAAGCCAGCA GTGAAACAAC AACTGAAAAT GAACAGAATT CAAACCAAGA ACAAAGTAAG 
ACAAAAAGCA GTGAGATTCT GTCATCAGAA CAAGAATCTG TTTATGCTAA AGGTGAACAA 
CATGTGGCTA CAATTTTGCC TTATTACCTC AATGTAATGG AGAATGCACC GCCTTTAACG 
GTCAATACCC CAGAATCTTT TATAACAGAC AGTATGAAAA CAGAAGAATC AAGTTCATCT 
TTACCAGTAA ATGAAACTCA AACACTGGAT ACAACGTGTG TAGGAAACAA ATCTGCTGCA 
AGTTCTGAAA GCTCCATGGC TGGCAGCCCT GATACAGAGT CACCTGTGTT AGTGAATGAA 
TATGAAGCTG GTTCTGGAAA TGTAAGTCAA AGATCTGATG AAGATGACTT TGTGAAAGTT 
GAAGACTTGC CCCTTAAACT TGCAGTGTAT TCAGAGGCAG ATTTAATGAA GAAAATGGCA 
ATGGAGGCCC AAACGAACAG TTTGTCTGAT GAATTACTGG GTGGAGGTGG AGCTCAAGAT 
CAAGAATTAG TAGGAGATGC CCAAACTTTG AAAGAACCTG AAACTTTTGG AGTTCAAAGT 
GCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_013366632.1 ) cDNA ORF clone, Columba livia(rock pigeon) -> XP_013222086.1 Columba livia pericentriolar material 1 (PCM1), transcript variant X1, mRNA.

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CloneID	OCo260085
Clone ID Related Accession (Same CDS sequence)	XM_013366632.1
Accession Version	XM_013366632.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6171bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-12
Organism	Columba livia(Rock pigeon)
Product	pericentriolar material 1 protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004973253.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Columba livia Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACAG GAGGTGGTCC CTTTGAAGAA GGCATGAATG ATCAAGACTT GCCTAGCTGG 
AGCAATGACA GCTTTGATGA CCGGCTGAAC AACACGGACT GGGGAAGTCA ACAGAAGAAA 
GCAAACAGAT CTTCAGAAAA AAACAAGAAA AAGCTTGGTG GGGAAGCTGA AACAAGGCTT 
ACTAATGATA TATCTCCAGA ATCCTCACCT GGAATAGGAC GACGAAAGAC CAGAACTCCT 
CATAGTTTTC CTCATGCTCG ATACATGACC CAGATGTCTG TTCCGGAGCA GGCTGAGCTA 
GAAAGGCTTA AACAAAGAAT AAACTTCAGT GATCTGGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGCA GGGCAACAGC TGCTAACAAC AAACGTCAAC TTAATGAAAA CAAAAAACCA 
TTCAACTTCC TGTCACTGCA GATAAACACT AACAAGAGCA AAGATCCTGC CTCATGTTCC 
CAAAAAAAGG AAAGTGGGGT GTCAGTCCAA CGTAAAGAGT TGTTTGGTGC TGCTCTGAGC 
AAGGATTTCT TGCAAAATTG TCAAGTCTCT GCTCAAGAAG ATGGAGGGGG AGAACCAGCT 
ATGGATAGTA GCCAGATTGT GAGCAGACTA GTTCAAATTC GCGACTATAT TGCTAAGGCC 
AGCTCCATGC GGGATGATCT TGTAGAGAAA AATGAAAGAT CGGCCAATGT TGAGCGTTTA 
TCACACCTTA TAGATCACCT TAAAGAGCAG GAGAAATCCT ATCTGAAATT TTTGCAAAAG 
ATGCTTGCTA GACAAAATGA GGAGGATGAT GTTCGGACTA TAGATTCAGC TGTGGGATCT 
GGTTCTGTAG GTGAGAGCAC ATCGCTAAAC ATTGATGTGC AGTCTGAGGC TTCAGATACC 
ACGGCTAGAG ATCCTCAACA GGAGGCTAAA GAGGAGTTGG AGAACTTGAA GAAGCAGCAT 
GATTTATTGA AAAGGATGCT AAAACAGCAA GAGCAATTAA AGGCTCTTCA AGGAAGACAG 
GCAGCTCTTC TTGCTTTGCA GCATAAAGCA GAGCAAGCAA TTGCTGTCAT GGATGATTCT 
GTTGTAACAG AGACTACAGG TAGTGTTTCA GGAGTAAGCC TTACATCAGA ACTGAATGAA 
GAATTGAATG ACTTAATTCA GCGCTTTCAC AACCAACTTC ATGATTCTCA GACACAGTCT 
GTACCAGACA ATAGAAGGCA AGCAGAAAGT CTTTCACTTA CTAGAGAGGT TTCACAAAGC 
AGAAACTCTT CAATGTCTGA ACGCCTGTCA GATGAGAAGG CGCAGCTTTT TAACAAGATG 
CGAATGTTGC AGGGTAAAAA GCAAAAAATG GACAAACTGT TAGGAGAACT TCATACACTT 
CGTGACCAAC ATCTAAATAA CTCCTCCTTT TTTCCTGCTT CAGGTTCTCC TCAAAGAAGT 
ATTGATCAAA GAAGTACAAC TTCAGCTGCT TCTGGTCCTG CAGGCATAGT AACTGTTGTC 
AATGGCGAAT CAAATAGCCT GGCATCTGCT CCCTATCCTC CTGATTCCCT GGTTTCTCAG 
AATGAGAGTG AAGAAGATGA CAATCTAAAT CCAACAGAAA AGCTTCAGAA ACTAAATGAA 
GTTCGTAAGA GGCTGAATGA GTTACGTGAG CTAGTTCACT ACTATGAGCA AACATCTGAT 
ATGATGACAG ATTCTGTGAA TGAAAACACT AAGGAAGAAG AAGAAACCGA AGAATCAGAA 
AGTGATTCTG AACGTGAGGA TCCACAGCCT GTTACAAATA TTAGAAATCC TCAAGGAATC 
AGTAGTTGGA GTGACATAAA TAGCAACTCA AATGTACAGT GTGGAACTAA TAACAGAGAT 
GGAAGACATC TTAATACAGA CTGTGAAATA AATAACAGAT CTGCTGCTAA CATAAGGACT 
CTAAAAATGT CGTCACCTTT AGACTGTCAT AATAGGGAGG ATGACAAAGA TATTGGCCTA 
CCCCAATGTG AAGATGAAGA AGTGGAAGAA GATAGAGTTA GTGAAGATTC TGCATCTAGT 
CACAGAAGCA GCCTGGGTGA TACTGCTGGA GATGCTGAGT TTGAGCAGAA GATCAATAGG 
CTTATAGCTG CAAAACAGAA ACTTAGACAA TTACAAAACC TTGCTGCTAT GGTGCAGGAT 
GATGATCCAG CACCTCAGAC AACAATTGCA AATGCATCTA ATATTGGTGA CTTGTTTTTG 
GGTGAGATGG AAGAGACAAA GCAGCAGCCA AACAATGTCC GAGCTAGTAC CAACAAATTA 
CAAAAAGATG TAGAACTGAA TGAAAAAGCA AGAGAGAAGT TTTATGAAGC TAAACTCCAG 
CAGCAGCAAC AGGAGCTTAA GCAGTTACAA GAAGAAAGAA GAAAACTGAT TGAAATTCAA 
GAAAAAATTC AGGTGTTACA GAAAGCTTGT CCTGATCTTC AATTGTCAGC TGGCTTGGGT 
GACTGCACAG CAAATAGACA GACTTCACCA GCAACATCAA CTCCAGCCAT GAATGACACA 
GCTGGCAAGC CTTCACTTGA ATTTGATGAA TCTGTACCAG TAGGCAATGA GTTATGGTCT 
GAGATGAGAA GACATGAGAT TTTAAGAGAA GAATTGCGAC AAAGAAGAAA GCAACTGGAA 
GCTCTAATGG CTGAGCATCA GAGGAGGAAA GAGCTCGCAG AAACAATATC TACTGTTGCT 
GCGTCTGTTA AAAGTGAAGG ATCAGAAGCT CGGCGTACTC CACAGCAGAG TAGGACTGAA 
AAGACAATGG CTACCTGGGG AGGTTCTACG CAGTGTGCAC TAGATGAAGA GGATGGAGAT 
GAAGATGGTT ATCTCTCTGA TGGAGCTGGT CAGGCAGAAG AAGATGATGA TGATGCGTCA 
AGTGCGAATG ACAGCTTCTC TGTTTATCCC AATAACAACG TACCAGAAAA TGCATATTTT 
GTTAAAGAAA ACAAGGATAG GTGGAAAAAT TACCGTCCTC TTTCAGCAGA TGGAAATTAT 
CGTCCATTGT CTAAGGCCAG ACAACAGCAA AACATAAGTA TGCGACGTCA GGAAAACCTT 
CGGTGGATGT CTGAACTTTC ATATGTGGAA GAAAAGGAAC GATGGCAAGA GCAGATCAAT 
CAGTTGAAGA AACAGCATGA ATTTAGTGTC AGCATTTGTC AAACTTTAAT GCAGGATCAG 
CAGACCCTCT CTTGTCTTCT ACAGACTTTG CTTACAGGCC CTTACAGTAT GATGCCCAAT 
AATGTTGCAT CTTCACAAGT ACATCTTATT ATGCATCAGC TAAACCAGTG TTACACTCAA 
CTGACATGGC AGCAGAATAA CGTCCAGAGG TTGAAACAAA TGTTAAATGA TCTTGTGCGT 
CAGCAAGAAG AACAACGGTA TGAAGAGAAG TCATCAAGAA AGGATAGAGG CAGTGGTGTA 
CCCCCACCTC CATCTCCTGT TTTCTGTCCG TTCAGCTTTC CTCCACAACC TTTGAACCTG 
TTTAATGTAC CAGGATTTAC TAATTTTTCT TCCTTTGCTC CAGATATTAA CTATAATCCA 
GTGTTTCCTT CTGGTTTTGG AGATTTTGCA CACAATATTT CTCCACACAG CAGTGAGCAG 
CAGCAGCAAC ATCCTCTAGA TCATAATGCA TCTGGGAAAA CTGAGTATAT GGCGTTCCCC 
AAACCTTTTG AAAGCAGTTC CTCTAATGGA GCGGAAAAAC AAAGGAGGAG TCACAGACAA 
CCTGAAGAGG AAATGGAAAA AAGATCAGCT TGGCTTAATG ATAGCCAGGA CGTGAAAAAA 
GATGATCAGT CTCAGCTGAA AGCAAGTTTT GCTGTTTCAG TACAAAACAT TGCTTCCAAT 
CATAAAAATC AGTCTGATAT GAGCAGAAGA AGAGAGTTTG ATGAAGAGTC TTTGGAGAGT 
TTTAGTAGCA TGCCTGATCC AGTAGACCCC ACTACTGTGA CAAAGACATT TAAATCTAGA 
AAAGCATCAG CACAAGCAAG TTTGGCATCA AAAGATAAAA CACCTAAATC GAAGAATAAG 
AGGAAGAGTT CTTCTCAGCT AAAAGGCAGA ATTACAAATA TTGATTATGA AAGTGCCAGT 
GCTTCTAGTG TGTGTGAACC CTGCAAGAGC AATAAAAGCA GACACTCTGA AGAGGTTGGT 
CATGCAAAGG TGTTCAGCAA AAGGAATCGG GAACAACTGG AAAAAATCAT TAAATACAGT 
AGATCTACAG AAATGTCTTC AGAAACTGGT AGTGATCTTT CTATGTTTGA AGCTTTACGA 
GACACAATTT ATTCTGAAGT GGCAACTCTT ATTTCTCAAA ATGAGTCTCG TCCCCACTTT 
CTTATTGAAC TTTTCCATGA GCTTCAGCTG CTAAATACAG ATTACCTGAG GCAAAGAGCT 
CTATACGCTC TACAGGATAT AGTGACCAGA CATTTATCTG AGAACAATGA AAAAGGGAAG 
TGCACAAAAT CACTGAATTC TGCAGCATGG ATAGCATCAA ATTCTGAACT CACTCCCAGT 
GAAAGCCTTG CTTCTACAGA TGATGAAACT TTTGGCAAAA ACTTTTCTAC AGAAGCATGT 
CAAGATTGTG AACAAAATGA TGCAGACAAT GGGAGTAGCA TGTCTACATC TTCAAATTTT 
GAACCCTTTG CCACTGATGA CCTTGGCAAC ACAGTGATTC ACTTAGATCA AGCTTTGGCT 
AGGATGAGGG AGTATGAGCG TATGAAAATT GAAGCTGAAA GTACCCTTGA CTCTGAGGGC 
TGCTCTAGTA ATTTTCAGGG TGCTTCTGCT GCTAAATTAG AAGGAGGTCA AAGTGCCAGT 
GAATGTGTTC CTGTGCCACA GTCAAGTGAA GCTTCTGCTG TTCCATGTCC TCATATAGAC 
ACTCAGCAGC TTGACCGGCA GATTAAAGCA ATTATGAAAG AGGTCATTCC TTTTCTGAAG 
GAACACATGG ATGAAGTATG CTCTTCGCAG TTACTGACAT CAGTAAGACG TATGGTCTTG 
ACCCTTACAC AACAAAATGA TGAAAGTAAA GAGTTTGTGA AGTTCTTTCA TAAGCAGCTT 
GGCAGTATAC TTCAGGATTC ACTGGCAAAA TTTGCTGGTA GAAAACTGAA AGATTGTGGA 
GAGGATCTTC TAGTGGAGAT CTCTGAAGTG TTATTTAATG AATTAGCCTT TTTTAAACTC 
ATGCAAGACT TGGACAACAA CAGTATTTCT GTAAAGCAGA GATGTAAAAG AAAAATAGAA 
GCTACTGAAG TGATACAGTC GTATGCTAAA GAGGCAAAAA AAGGTCTCCA GGTGGATGTT 
TGTTCATCTG CTGAAGATGT CGATGAGGAC AAAGACAAGG ATGAGACTGA AACTGTTAAA 
CAAGTACAGG ACTCAGAAAT GTATGATGGG AATAGAGTTC CTGAAAGTAT TAGGTCTGAT 
GCATCTGATC AGGAGGAAGA TGATGAAAGT GAAAGCGGTC CAGTGGCAAT AAGTTTATCA 
AAAGCAGAAA CCCAAGCTCT GACTAACTAT GGTAGTGGAG AAGATGAGAA TGAAGATGAA 
GAAATAGAAT TTGAGGAAGG ACCTGTTGAT GTGCAGACGT CATTACAAGC CAGCAGTGAA 
ACAACAACTG AAAATGAACA GAATTCAAAC CAAGAACAAA GTAAGACAAA AAGCAGTGAG 
ATTCTGTCAT CAGAACAAGA ATCTGTTTAT GCTAAAGGTG AACAACATGT GGCTACAATT 
TTGCCTTATT ACCTCAATGT AATGGAGAAT GCACCGCCTT TAACGGTCAA TACCCCAGAA 
TCTTTTATAA CAGACAGTAT GAAAACAGAA GAATCAAGTT CATCTTTACC AGTAAATGAA 
ACTCAAACAC TGGATACAAC GTGTGTAGGA AACAAATCTG CTGCAAGTTC TGAAAGCTCC 
ATGGCTGGCA GCCCTGATAC AGAGTCACCT GTGTTAGTGA ATGAATATGA AGCTGGTTCT 
GGAAATGTAA GTCAAAGATC TGATGAAGAT GACTTTGTGA AAGTTGAAGA CTTGCCCCTT 
AAACTTGCAG TGTATTCAGA GGCAGATTTA ATGAAGAAAA TGGCAATGGA GGCCCAAACG 
AACAGTTTGT CTGATGAATT ACTGGGTGGA GGTGGAGCTC AAGATCAAGA ATTAGTAGGA 
GATGCCCAAA CTTTGAAAGA ACCTGAAACT TTTGGAGTTC AAAGTGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013222086.1
CDS	96..6266
Translation	MATGGGPFEEGMNDQDLPSWSNDSFDDRLNNTDWGSQQKKANRSSEKNKKKLGGEAETRLTNDISPESSPGIGRRKTRTPHSFPHARYMTQMSVPEQAELERLKQRINFSDLDQRSIGSDSQGRATAANNKRQLNENKKPFNFLSLQINTNKSKDPASCSQKKESGVSVQRKELFGAALSKDFLQNCQVSAQEDGGGEPAMDSSQIVSRLVQIRDYIAKASSMRDDLVEKNERSANVERLSHLIDHLKEQEKSYLKFLQKMLARQNEEDDVRTIDSAVGSGSVGESTSLNIDVQSEASDTTARDPQQEAKEELENLKKQHDLLKRMLKQQEQLKALQGRQAALLALQHKAEQAIAVMDDSVVTETTGSVSGVSLTSELNEELNDLIQRFHNQLHDSQTQSVPDNRRQAESLSLTREVSQSRNSSMSERLSDEKAQLFNKMRMLQGKKQKMDKLLGELHTLRDQHLNNSSFFPASGSPQRSIDQRSTTSAASGPAGIVTVVNGESNSLASAPYPPDSLVSQNESEEDDNLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDSVNENTKEEEETEESESDSEREDPQPVTNIRNPQGISSWSDINSNSNVQCGTNNRDGRHLNTDCEINNRSAANIRTLKMSSPLDCHNREDDKDIGLPQCEDEEVEEDRVSEDSASSHRSSLGDTAGDAEFEQKINRLIAAKQKLRQLQNLAAMVQDDDPAPQTTIANASNIGDLFLGEMEETKQQPNNVRASTNKLQKDVELNEKAREKFYEAKLQQQQQELKQLQEERRKLIEIQEKIQVLQKACPDLQLSAGLGDCTANRQTSPATSTPAMNDTAGKPSLEFDESVPVGNELWSEMRRHEILREELRQRRKQLEALMAEHQRRKELAETISTVAASVKSEGSEARRTPQQSRTEKTMATWGGSTQCALDEEDGDEDGYLSDGAGQAEEDDDDASSANDSFSVYPNNNVPENAYFVKENKDRWKNYRPLSADGNYRPLSKARQQQNISMRRQENLRWMSELSYVEEKERWQEQINQLKKQHEFSVSICQTLMQDQQTLSCLLQTLLTGPYSMMPNNVASSQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNDLVRQQEEQRYEEKSSRKDRGSGVPPPPSPVFCPFSFPPQPLNLFNVPGFTNFSSFAPDINYNPVFPSGFGDFAHNISPHSSEQQQQHPLDHNASGKTEYMAFPKPFESSSSNGAEKQRRSHRQPEEEMEKRSAWLNDSQDVKKDDQSQLKASFAVSVQNIASNHKNQSDMSRRREFDEESLESFSSMPDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKNKRKSSSQLKGRITNIDYESASASSVCEPCKSNKSRHSEEVGHAKVFSKRNREQLEKIIKYSRSTEMSSETGSDLSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHLSENNEKGKCTKSLNSAAWIASNSELTPSESLASTDDETFGKNFSTEACQDCEQNDADNGSSMSTSSNFEPFATDDLGNTVIHLDQALARMREYERMKIEAESTLDSEGCSSNFQGASAAKLEGGQSASECVPVPQSSEASAVPCPHIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSISVKQRCKRKIEATEVIQSYAKEAKKGLQVDVCSSAEDVDEDKDKDETETVKQVQDSEMYDGNRVPESIRSDASDQEEDDESESGPVAISLSKAETQALTNYGSGEDENEDEEIEFEEGPVDVQTSLQASSETTTENEQNSNQEQSKTKSSEILSSEQESVYAKGEQHVATILPYYLNVMENAPPLTVNTPESFITDSMKTEESSSSLPVNETQTLDTTCVGNKSAASSESSMAGSPDTESPVLVNEYEAGSGNVSQRSDEDDFVKVEDLPLKLAVYSEADLMKKMAMEAQTNSLSDELLGGGGAQDQELVGDAQTLKEPETFGVQSA

Target ORF information:

RefSeq Version	XM_013366632.1
Organism	Columba livia(Rock pigeon)
Definition	Columba livia pericentriolar material 1 (PCM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013366632.1

ORF Insert Sequence:

ATGGCAACAG GAGGTGGTCC CTTTGAAGAA GGCATGAATG ATCAAGACTT GCCTAGCTGG 
AGCAATGACA GCTTTGATGA CCGGCTGAAC AACACGGACT GGGGAAGTCA ACAGAAGAAA 
GCAAACAGAT CTTCAGAAAA AAACAAGAAA AAGCTTGGTG GGGAAGCTGA AACAAGGCTT 
ACTAATGATA TATCTCCAGA ATCCTCACCT GGAATAGGAC GACGAAAGAC CAGAACTCCT 
CATAGTTTTC CTCATGCTCG ATACATGACC CAGATGTCTG TTCCGGAGCA GGCTGAGCTA 
GAAAGGCTTA AACAAAGAAT AAACTTCAGT GATCTGGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGCA GGGCAACAGC TGCTAACAAC AAACGTCAAC TTAATGAAAA CAAAAAACCA 
TTCAACTTCC TGTCACTGCA GATAAACACT AACAAGAGCA AAGATCCTGC CTCATGTTCC 
CAAAAAAAGG AAAGTGGGGT GTCAGTCCAA CGTAAAGAGT TGTTTGGTGC TGCTCTGAGC 
AAGGATTTCT TGCAAAATTG TCAAGTCTCT GCTCAAGAAG ATGGAGGGGG AGAACCAGCT 
ATGGATAGTA GCCAGATTGT GAGCAGACTA GTTCAAATTC GCGACTATAT TGCTAAGGCC 
AGCTCCATGC GGGATGATCT TGTAGAGAAA AATGAAAGAT CGGCCAATGT TGAGCGTTTA 
TCACACCTTA TAGATCACCT TAAAGAGCAG GAGAAATCCT ATCTGAAATT TTTGCAAAAG 
ATGCTTGCTA GACAAAATGA GGAGGATGAT GTTCGGACTA TAGATTCAGC TGTGGGATCT 
GGTTCTGTAG GTGAGAGCAC ATCGCTAAAC ATTGATGTGC AGTCTGAGGC TTCAGATACC 
ACGGCTAGAG ATCCTCAACA GGAGGCTAAA GAGGAGTTGG AGAACTTGAA GAAGCAGCAT 
GATTTATTGA AAAGGATGCT AAAACAGCAA GAGCAATTAA AGGCTCTTCA AGGAAGACAG 
GCAGCTCTTC TTGCTTTGCA GCATAAAGCA GAGCAAGCAA TTGCTGTCAT GGATGATTCT 
GTTGTAACAG AGACTACAGG TAGTGTTTCA GGAGTAAGCC TTACATCAGA ACTGAATGAA 
GAATTGAATG ACTTAATTCA GCGCTTTCAC AACCAACTTC ATGATTCTCA GACACAGTCT 
GTACCAGACA ATAGAAGGCA AGCAGAAAGT CTTTCACTTA CTAGAGAGGT TTCACAAAGC 
AGAAACTCTT CAATGTCTGA ACGCCTGTCA GATGAGAAGG CGCAGCTTTT TAACAAGATG 
CGAATGTTGC AGGGTAAAAA GCAAAAAATG GACAAACTGT TAGGAGAACT TCATACACTT 
CGTGACCAAC ATCTAAATAA CTCCTCCTTT TTTCCTGCTT CAGGTTCTCC TCAAAGAAGT 
ATTGATCAAA GAAGTACAAC TTCAGCTGCT TCTGGTCCTG CAGGCATAGT AACTGTTGTC 
AATGGCGAAT CAAATAGCCT GGCATCTGCT CCCTATCCTC CTGATTCCCT GGTTTCTCAG 
AATGAGAGTG AAGAAGATGA CAATCTAAAT CCAACAGAAA AGCTTCAGAA ACTAAATGAA 
GTTCGTAAGA GGCTGAATGA GTTACGTGAG CTAGTTCACT ACTATGAGCA AACATCTGAT 
ATGATGACAG ATTCTGTGAA TGAAAACACT AAGGAAGAAG AAGAAACCGA AGAATCAGAA 
AGTGATTCTG AACGTGAGGA TCCACAGCCT GTTACAAATA TTAGAAATCC TCAAGGAATC 
AGTAGTTGGA GTGACATAAA TAGCAACTCA AATGTACAGT GTGGAACTAA TAACAGAGAT 
GGAAGACATC TTAATACAGA CTGTGAAATA AATAACAGAT CTGCTGCTAA CATAAGGACT 
CTAAAAATGT CGTCACCTTT AGACTGTCAT AATAGGGAGG ATGACAAAGA TATTGGCCTA 
CCCCAATGTG AAGATGAAGA AGTGGAAGAA GATAGAGTTA GTGAAGATTC TGCATCTAGT 
CACAGAAGCA GCCTGGGTGA TACTGCTGGA GATGCTGAGT TTGAGCAGAA GATCAATAGG 
CTTATAGCTG CAAAACAGAA ACTTAGACAA TTACAAAACC TTGCTGCTAT GGTGCAGGAT 
GATGATCCAG CACCTCAGAC AACAATTGCA AATGCATCTA ATATTGGTGA CTTGTTTTTG 
GGTGAGATGG AAGAGACAAA GCAGCAGCCA AACAATGTCC GAGCTAGTAC CAACAAATTA 
CAAAAAGATG TAGAACTGAA TGAAAAAGCA AGAGAGAAGT TTTATGAAGC TAAACTCCAG 
CAGCAGCAAC AGGAGCTTAA GCAGTTACAA GAAGAAAGAA GAAAACTGAT TGAAATTCAA 
GAAAAAATTC AGGTGTTACA GAAAGCTTGT CCTGATCTTC AATTGTCAGC TGGCTTGGGT 
GACTGCACAG CAAATAGACA GACTTCACCA GCAACATCAA CTCCAGCCAT GAATGACACA 
GCTGGCAAGC CTTCACTTGA ATTTGATGAA TCTGTACCAG TAGGCAATGA GTTATGGTCT 
GAGATGAGAA GACATGAGAT TTTAAGAGAA GAATTGCGAC AAAGAAGAAA GCAACTGGAA 
GCTCTAATGG CTGAGCATCA GAGGAGGAAA GAGCTCGCAG AAACAATATC TACTGTTGCT 
GCGTCTGTTA AAAGTGAAGG ATCAGAAGCT CGGCGTACTC CACAGCAGAG TAGGACTGAA 
AAGACAATGG CTACCTGGGG AGGTTCTACG CAGTGTGCAC TAGATGAAGA GGATGGAGAT 
GAAGATGGTT ATCTCTCTGA TGGAGCTGGT CAGGCAGAAG AAGATGATGA TGATGCGTCA 
AGTGCGAATG ACAGCTTCTC TGTTTATCCC AATAACAACG TACCAGAAAA TGCATATTTT 
GTTAAAGAAA ACAAGGATAG GTGGAAAAAT TACCGTCCTC TTTCAGCAGA TGGAAATTAT 
CGTCCATTGT CTAAGGCCAG ACAACAGCAA AACATAAGTA TGCGACGTCA GGAAAACCTT 
CGGTGGATGT CTGAACTTTC ATATGTGGAA GAAAAGGAAC GATGGCAAGA GCAGATCAAT 
CAGTTGAAGA AACAGCATGA ATTTAGTGTC AGCATTTGTC AAACTTTAAT GCAGGATCAG 
CAGACCCTCT CTTGTCTTCT ACAGACTTTG CTTACAGGCC CTTACAGTAT GATGCCCAAT 
AATGTTGCAT CTTCACAAGT ACATCTTATT ATGCATCAGC TAAACCAGTG TTACACTCAA 
CTGACATGGC AGCAGAATAA CGTCCAGAGG TTGAAACAAA TGTTAAATGA TCTTGTGCGT 
CAGCAAGAAG AACAACGGTA TGAAGAGAAG TCATCAAGAA AGGATAGAGG CAGTGGTGTA 
CCCCCACCTC CATCTCCTGT TTTCTGTCCG TTCAGCTTTC CTCCACAACC TTTGAACCTG 
TTTAATGTAC CAGGATTTAC TAATTTTTCT TCCTTTGCTC CAGATATTAA CTATAATCCA 
GTGTTTCCTT CTGGTTTTGG AGATTTTGCA CACAATATTT CTCCACACAG CAGTGAGCAG 
CAGCAGCAAC ATCCTCTAGA TCATAATGCA TCTGGGAAAA CTGAGTATAT GGCGTTCCCC 
AAACCTTTTG AAAGCAGTTC CTCTAATGGA GCGGAAAAAC AAAGGAGGAG TCACAGACAA 
CCTGAAGAGG AAATGGAAAA AAGATCAGCT TGGCTTAATG ATAGCCAGGA CGTGAAAAAA 
GATGATCAGT CTCAGCTGAA AGCAAGTTTT GCTGTTTCAG TACAAAACAT TGCTTCCAAT 
CATAAAAATC AGTCTGATAT GAGCAGAAGA AGAGAGTTTG ATGAAGAGTC TTTGGAGAGT 
TTTAGTAGCA TGCCTGATCC AGTAGACCCC ACTACTGTGA CAAAGACATT TAAATCTAGA 
AAAGCATCAG CACAAGCAAG TTTGGCATCA AAAGATAAAA CACCTAAATC GAAGAATAAG 
AGGAAGAGTT CTTCTCAGCT AAAAGGCAGA ATTACAAATA TTGATTATGA AAGTGCCAGT 
GCTTCTAGTG TGTGTGAACC CTGCAAGAGC AATAAAAGCA GACACTCTGA AGAGGTTGGT 
CATGCAAAGG TGTTCAGCAA AAGGAATCGG GAACAACTGG AAAAAATCAT TAAATACAGT 
AGATCTACAG AAATGTCTTC AGAAACTGGT AGTGATCTTT CTATGTTTGA AGCTTTACGA 
GACACAATTT ATTCTGAAGT GGCAACTCTT ATTTCTCAAA ATGAGTCTCG TCCCCACTTT 
CTTATTGAAC TTTTCCATGA GCTTCAGCTG CTAAATACAG ATTACCTGAG GCAAAGAGCT 
CTATACGCTC TACAGGATAT AGTGACCAGA CATTTATCTG AGAACAATGA AAAAGGGAAG 
TGCACAAAAT CACTGAATTC TGCAGCATGG ATAGCATCAA ATTCTGAACT CACTCCCAGT 
GAAAGCCTTG CTTCTACAGA TGATGAAACT TTTGGCAAAA ACTTTTCTAC AGAAGCATGT 
CAAGATTGTG AACAAAATGA TGCAGACAAT GGGAGTAGCA TGTCTACATC TTCAAATTTT 
GAACCCTTTG CCACTGATGA CCTTGGCAAC ACAGTGATTC ACTTAGATCA AGCTTTGGCT 
AGGATGAGGG AGTATGAGCG TATGAAAATT GAAGCTGAAA GTACCCTTGA CTCTGAGGGC 
TGCTCTAGTA ATTTTCAGGG TGCTTCTGCT GCTAAATTAG AAGGAGGTCA AAGTGCCAGT 
GAATGTGTTC CTGTGCCACA GTCAAGTGAA GCTTCTGCTG TTCCATGTCC TCATATAGAC 
ACTCAGCAGC TTGACCGGCA GATTAAAGCA ATTATGAAAG AGGTCATTCC TTTTCTGAAG 
GAACACATGG ATGAAGTATG CTCTTCGCAG TTACTGACAT CAGTAAGACG TATGGTCTTG 
ACCCTTACAC AACAAAATGA TGAAAGTAAA GAGTTTGTGA AGTTCTTTCA TAAGCAGCTT 
GGCAGTATAC TTCAGGATTC ACTGGCAAAA TTTGCTGGTA GAAAACTGAA AGATTGTGGA 
GAGGATCTTC TAGTGGAGAT CTCTGAAGTG TTATTTAATG AATTAGCCTT TTTTAAACTC 
ATGCAAGACT TGGACAACAA CAGTATTTCT GTAAAGCAGA GATGTAAAAG AAAAATAGAA 
GCTACTGAAG TGATACAGTC GTATGCTAAA GAGGCAAAAA AAGGTCTCCA GGTGGATGTT 
TGTTCATCTG CTGAAGATGT CGATGAGGAC AAAGACAAGG ATGAGACTGA AACTGTTAAA 
CAAGTACAGG ACTCAGAAAT GTATGATGGG AATAGAGTTC CTGAAAGTAT TAGGTCTGAT 
GCATCTGATC AGGAGGAAGA TGATGAAAGT GAAAGCGGTC CAGTGGCAAT AAGTTTATCA 
AAAGCAGAAA CCCAAGCTCT GACTAACTAT GGTAGTGGAG AAGATGAGAA TGAAGATGAA 
GAAATAGAAT TTGAGGAAGG ACCTGTTGAT GTGCAGACGT CATTACAAGC CAGCAGTGAA 
ACAACAACTG AAAATGAACA GAATTCAAAC CAAGAACAAA GTAAGACAAA AAGCAGTGAG 
ATTCTGTCAT CAGAACAAGA ATCTGTTTAT GCTAAAGGTG AACAACATGT GGCTACAATT 
TTGCCTTATT ACCTCAATGT AATGGAGAAT GCACCGCCTT TAACGGTCAA TACCCCAGAA 
TCTTTTATAA CAGACAGTAT GAAAACAGAA GAATCAAGTT CATCTTTACC AGTAAATGAA 
ACTCAAACAC TGGATACAAC GTGTGTAGGA AACAAATCTG CTGCAAGTTC TGAAAGCTCC 
ATGGCTGGCA GCCCTGATAC AGAGTCACCT GTGTTAGTGA ATGAATATGA AGCTGGTTCT 
GGAAATGTAA GTCAAAGATC TGATGAAGAT GACTTTGTGA AAGTTGAAGA CTTGCCCCTT 
AAACTTGCAG TGTATTCAGA GGCAGATTTA ATGAAGAAAA TGGCAATGGA GGCCCAAACG 
AACAGTTTGT CTGATGAATT ACTGGGTGGA GGTGGAGCTC AAGATCAAGA ATTAGTAGGA 
GATGCCCAAA CTTTGAAAGA ACCTGAAACT TTTGGAGTTC AAAGTGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_021285854.1 ) cDNA ORF clone, Columba livia(rock pigeon) -> XP_021141529.1 Columba livia pericentriolar material 1 (PCM1), transcript variant X1, mRNA.

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CloneID	OCo293160
Clone ID Related Accession (Same CDS sequence)	XM_021285854.1 , XM_021285857.1 , XM_021285856.1 , XM_021285855.1
Accession Version	XM_021285854.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-23
Organism	Columba livia(rock pigeon)
Product	pericentriolar material 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004973253.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Columba livia Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACAG GAGGTGGTCC CTTTGAAGAA GGCATGAATG ATCAAGACTT GCCTAGCTGG 
AGCAATGACA GCTTTGATGA CCGGCTGAAC AACACGGACT GGGGAAGTCA ACAGAAGAAA 
GCAAACAGAT CTTCAGAAAA AAACAAGAAA AAGCTTGGTG GGGAAGCTGA AACAAGGCTT 
ACTAATGATA TATCTCCAGA ATCCTCACCT GGAATAGGAC GACGAAAGAC CAGAACTCCT 
CATAGTTTTC CTCATGCTCG ATACATGACC CAGATGTCTG TTCCGGAGCA GGCTGAGCTA 
GAAAGGCTTA AACAAAGAAT AAACTTCAGT GATCTGGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGCA GGGCAACAGC TGCTAACAAC AAACGTCAAC TTAATGAAAA CAAAAAACCA 
TTCAACTTCC TGTCACTGCA GATAAACACT AACAAGAGCA AAGATCCTGC CTCATGTTCC 
CAAAAAAAGG AAAGTGGGGT GTCAGTCCAA CGTAAAGAGT TGTTTGGTGC TGCTCTGAGC 
AAGGATTTCT TGCAAAATTG TCAAGTCTCT GCTCAAGAAG ATGGAGGGGG AGAACCAGCT 
ATGGATAGTA GCCAGATTGT GAGCAGACTA GTTCAAATTC GCGACTATAT TGCTAAGGCC 
AGCTCCATGC GGGATGATCT TGTAGAGAAA AATGAAAGAT CGGCCAATGT TGAGCGTTTA 
TCACACCTTA TAGATCACCT TAAAGAGCAG GAGAAATCCT ATCTGAAATT TTTGCAAAAG 
ATGCTTGCTA GACAAAATGA GGAGGATGAT GTTCGGACTA TAGATTCAGC TGTGGGATCT 
GGTTCTGTAG GTGAGAGCAC ATCGCTAAAC ATTGATGTGC AGTCTGAGGC TTCAGATACC 
ACGGAGGTAT CTTTTAGTTT GAGCATTCGG CCCTGCATTG AGGACAAACT AGGGAATTCA 
GCTTCACAAG AACAGGTTAC AGACTTTGAT GTTACTGCAA GCCCTAAAGG GAAAAATGAC 
AGAGCTGCCC TGAATGACAG GGAAATCTGG CCTTGTAGGA TTAATAGCCG AGAACATGGA 
TTGCCTTCAA AGGCTAGAGA TCCTCAACAG GAGGCTAAAG AGGAGTTGGA GAACTTGAAG 
AAGCAGCATG ATTTATTGAA AAGGATGCTA AAACAGCAAG AGCAATTAAA GGCTCTTCAA 
GGAAGACAGG CAGCTCTTCT TGCTTTGCAG CATAAAGCAG AGCAAGCAAT TGCTGTCATG 
GATGATTCTG TTGTAACAGA GACTACAGGT AGTGTTTCAG GAGTAAGCCT TACATCAGAA 
CTGAATGAAG AATTGAATGA CTTAATTCAG CGCTTTCACA ACCAACTTCA TGATTCTCAG 
ACACAGTCTG TACCAGACAA TAGAAGGCAA GCAGAAAGTC TTTCACTTAC TAGAGAGGTT 
TCACAAAGCA GAAACTCTTC AATGTCTGAA CGCCTGTCAG ATGAGAAGGC GCAGCTTTTT 
AACAAGATGC GAATGTTGCA GGGTAAAAAG CAAAAAATGG ACAAACTGTT AGGAGAACTT 
CATACACTTC GTGACCAACA TCTAAATAAC TCCTCCTTTT TTCCTGCTTC AGGTTCTCCT 
CAAAGAAGTA TTGATCAAAG AAGTACAACT TCAGCTGCTT CTGGTCCTGC AGGCATAGTA 
ACTGTTGTCA ATGGCGAATC AAATAGCCTG GCATCTGCTC CCTATCCTCC TGATTCCCTG 
GTTTCTCAGA ATGAGAGTGA AGAAGATGAC AATCTAAATC CAACAGAAAA GCTTCAGAAA 
CTAAATGAAG TTCGTAAGAG GCTGAATGAG TTACGTGAGC TAGTTCACTA CTATGAGCAA 
ACATCTGATA TGATGACAGA TTCTGTGAAT GAAAACACTA AGGAAGAAGA AGAAACCGAA 
GAATCAGAAA GTGATTCTGA ACGTGAGGAT CCACAGCCTG TTACAAATAT TAGAAATCCT 
CAAGGAATCA GTAGTTGGAG TGACATAAAT AGCAACTCAA ATGTACAGTG TGGAACTAAT 
AACAGAGATG GAAGACATCT TAATACAGAC TGTGAAATAA ATAACAGATC TGCTGCTAAC 
ATAAGGACTC TAAAAATGTC GTCACCTTTA GACTGTCATA ATAGGGAGGA TGACAAAGAT 
ATTGGCCTAC CCCAATGTGA AGATGAAGAA GTGGAAGAAG ATAGAGTTAG TGAAGATTCT 
GCATCTAGTC ACAGAAGCAG CCTGGGTGAT ACTGCTGGAG ATGCTGAGTT TGAGCAGAAG 
ATCAATAGGC TTATAGCTGC AAAACAGAAA CTTAGACAAT TACAAAACCT TGCTGCTATG 
GTGCAGGATG ATGATCCAGC ACCTCAGACA ACAATTGCAA ATGCATCTAA TATTGGTGAC 
TTGTTTTTGG GTGAGATGGA AGAGACAAAG CAGCAGCCAA ACAATGTCCG AGCTAGTACC 
AACAAATTAC AAAAAGATGT AGAACTGAAT GAAAAAGCAA GAGAGAAGTT TTATGAAGCT 
AAACTCCAGC AGCAGCAACA GGAGCTTAAG CAGTTACAAG AAGAAAGAAG AAAACTGATT 
GAAATTCAAG AAAAAATTCA GGTGTTACAG AAAGCTTGTC CTGATCTTCA ATTGTCAGCT 
GGCTTGGGTG ACTGCACAGC AAATAGACAG ACTTCACCAG CAACATCAAC TCCAGCCATG 
AATGACACAG CTGGCAAGCC TTCACTTGAA TTTGATGAAT CTGTACCAGT AGGCAATGAG 
TTATGGTCTG AGATGAGAAG ACATGAGATT TTAAGAGAAG AATTGCGACA AAGAAGAAAG 
CAACTGGAAG CTCTAATGGC TGAGCATCAG AGGAGGAAAG AGCTCGCAGA AACAATATCT 
ACTGTTGCTG CGTCTGTTAA AAGTGAAGGA TCAGAAGCTC GGCGTACTCC ACAGCAGAGT 
AGGACTGAAA ATAGGACAAT GGCTACCTGG GGAGGTTCTA CGCAGTGTGC ACTAGATGAA 
GAGGATGGAG ATGAAGATGG TTATCTCTCT GATGGAGCTG GTCAGGCAGA AGAAGATGAT 
GATGATGCGT CAAGTGCGAA TGACAGCTTC TCTGTTTATC CCAATAACAA CGTACCAGAA 
AATGCATATT TTGTTAAAGA AAACAAGGAT AGGTGGAAAA ATTACCGTCC TCTTTCAGCA 
GATGGAAATT ATCGTCCATT GTCTAAGGCC AGACAACAGC AAAACATAAG TATGCGACGT 
CAGGAAAACC TTCGGTGGAT GTCTGAACTT TCATATGTGG AAGAAAAGGA ACGATGGCAA 
GAGCAGATCA ATCAGTTGAA GAAACAGCAT GAATTTAGTG TCAGCATTTG TCAAACTTTA 
ATGCAGGATC AGCAGACCCT CTCTTGTCTT CTACAGACTT TGCTTACAGG CCCTTACAGT 
ATGATGCCCA ATAATGTTGC ATCTTCACAA GTACATCTTA TTATGCATCA GCTAAACCAG 
TGTTACACTC AACTGACATG GCAGCAGAAT AACGTCCAGA GGTTGAAACA AATGTTAAAT 
GATCTTGTGC GTCAGCAAGA AGAACAACGG TATGAAGAGA AGTCATCAAG AAAGGATAGA 
GGCAGTGGTG TACCCCCACC TCCATCTCCT GTTTTCTGTC CGTTCAGCTT TCCTCCACAA 
CCTTTGAACC TGTTTAATGT ACCAGGATTT ACTAATTTTT CTTCCTTTGC TCCAGATATT 
AACTATAATC CAGTGTTTCC TTCTGGTTTT GGAGATTTTG CACACAATAT TTCTCCACAC 
AGCAGTGAGC AGCAGCAGCA ACATCCTCTA GATCATAATG CATCTGGGAA AACTGAGTAT 
ATGGCGTTCC CCAAACCTTT TGAAAGCAGT TCCTCTAATG GAGCGGAAAA ACAAAGGAGG 
AGTCACAGAC AACCTGAAGA GGAAATGGAA AAAAGATCAG CTTGGCTTAA TGATAGCCAG 
GACGTGAAAA AAGATGATCA GTCTCAGCTG AAAGCAAGTT TTGCTGTTTC AGTACAAAAC 
ATTGCTTCCA ATCATAAAAA TCAGTCTGAT ATGAGCAGAA GAAGAGAGTT TGATGAAGAG 
TCTTTGGAGA GTTTTAGTAG CATGCCTGAT CCAGTAGACC CCACTACTGT GACAAAGACA 
TTTAAATCTA GAAAAGCATC AGCACAAGCA AGTTTGGCAT CAAAAGATAA AACACCTAAA 
TCGAAGAATA AGAGGAAGAG TTCTTCTCAG CTAAAAGGCA GAATTACAAA TATTGATTAT 
GAAAGTGCCA GTGCTTCTAG TGTGTGTGAA CCCTGCAAGA GCAATAAAAG CAGACACTCT 
GAAGAGGTTG GTCATGCAAA GGTGTTCAGC AAAAGGAATC GGGAACAACT GGAAAAAATC 
ATTAAATACA GTAGATCTAC AGAAATGTCT TCAGCGCATG CTAGGAGAAT TCTGCAGCAG 
TCTAACAGAA ATGCATGCAT TGAAGCGCCA GAAACTGGTA GTGATCTTTC TATGTTTGAA 
GCTTTACGAG ACACAATTTA TTCTGAAGTG GCAACTCTTA TTTCTCAAAA TGAGTCTCGT 
CCCCACTTTC TTATTGAACT TTTCCATGAG CTTCAGCTGC TAAATACAGA TTACCTGAGG 
CAAAGAGCTC TATACGCTCT ACAGGATATA GTGACCAGAC ATTTATCTGA GAACAATGAA 
AAAGGGAAGT GCACAAAATC ACTGAATTCT GCAGCATGGA TAGCATCAAA TTCTGAACTC 
ACTCCCAGTG AAAGCCTTGC TTCTACAGAT GATGAAACTT TTGGCAAAAA CTTTTCTACA 
GAAGCATGTC AAGATTGTGA ACAAAATGAT GCAGACAATG GGAGTAGCAT GTCTACATCT 
TCAAATTTTG AACCCTTTGC CACTGATGAC CTTGGCAACA CAGTGATTCA CTTAGATCAA 
GCTTTGGCTA GGATGAGGGA GTATGAGCGT ATGAAAATTG AAGCTGAAAG TACCCTTGAC 
TCTGAGGGCT GCTCTAGTAA TTTTCAGGGT GCTTCTGCTG CTAAATTAGA AGGAGGTCAA 
AGTGCCAGTG AATGTGTTCC TGTGCCACAG TCAAGTGAAG CTTCTGCTGT TCCATGTCCT 
CATATAGACA CTCAGCAGCT TGACCGGCAG ATTAAAGCAA TTATGAAAGA GGTCATTCCT 
TTTCTGAAGG AACACATGGA TGAAGTATGC TCTTCGCAGT TACTGACATC AGTAAGACGT 
ATGGTCTTGA CCCTTACACA ACAAAATGAT GAAAGTAAAG AGTTTGTGAA GTTCTTTCAT 
AAGCAGCTTG GCAGTATACT TCAGGATTCA CTGGCAAAAT TTGCTGGTAG AAAACTGAAA 
GATTGTGGAG AGGATCTTCT AGTGGAGATC TCTGAAGTGT TATTTAATGA ATTAGCCTTT 
TTTAAACTCA TGCAAGACTT GGACAACAAC AGTATTTCTG TAAAGCAGAG ATGTAAAAGA 
AAAATAGAAG CTACTGAAGT GATACAGTCG TATGCTAAAG AGGCAAAAAA AGGTCTCCAG 
GTGGATGTTT GTTCATCTGC TGAAGATGTC GATGAGGACA AAGACAAGGA TGAGACTGAA 
ACTGTTAAAC AAGTACAGGA CTCAGAAATG TATGATGGGA ATAGAGTTCC TGAAAGTATT 
AGGTCTGATG CATCTGATCA GGAGGAAGAT GATGAAAGTG AAAGCGGTCC AGTGGCAATA 
AGTTTATCAA AAGCAGAAAC CCAAGCTCTG ACTAACTATG GTAGTGGAGA AGATGAGAAT 
GAAGATGAAG AAATAGAATT TGAGGAAGGA CCTGTTGATG TGCAGACGTC ATTACAAGCC 
AGCAGTGAAA CAACAACTGA AAATGAACAG AATTCAAACC AAGAACAAAG TAAGACAAAA 
AGCAGTGAGA TTCTGTCATC AGAACAAGAA TCTGTTTATG CTAAAGGTGA ACAACATGTG 
GCTACAATTT TGCCTTATTA CCTCAATGTA ATGGAGAATG CACCGCCTTT AACGGTCAAT 
ACCCCAGAAT CTTTTATAAC AGACAGTATG AAAACAGAAG AATCAAGTTC ATCTTTACCA 
GTAAATGAAA CTCAAACACT GGATACAACG TGTGTAGGAA ACAAATCTGC TGCAAGTTCT 
GAAAGCTCCA TGGCTGGCAG CCCTGATACA GAGTCACCTG TGTTAGTGAA TGAATATGAA 
GCTGGTTCTG GAAATGTAAG TCAAAGATCT GATGAAGATG ACTTTGTGAA AGTTGAAGAC 
TTGCCCCTTA AACTTGCAGT GTATTCAGAG GCAGATTTAA TGAAGAAAAT GGCAATGGAG 
GCCCAAACGA ACAGTTTGTC TGATGAATTA CTGGGTGGAG GTGGAGCTCA AGATCAAGAA 
TTAGTAGGAG ATGCCCAAAC TTTGAAAGAA CCTGAAACTT TTGGAGTTCA AAGTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021141529.1
CDS	140..6559
Translation	MATGGGPFEEGMNDQDLPSWSNDSFDDRLNNTDWGSQQKKANRSSEKNKKKLGGEAETRLTNDISPESSPGIGRRKTRTPHSFPHARYMTQMSVPEQAELERLKQRINFSDLDQRSIGSDSQGRATAANNKRQLNENKKPFNFLSLQINTNKSKDPASCSQKKESGVSVQRKELFGAALSKDFLQNCQVSAQEDGGGEPAMDSSQIVSRLVQIRDYIAKASSMRDDLVEKNERSANVERLSHLIDHLKEQEKSYLKFLQKMLARQNEEDDVRTIDSAVGSGSVGESTSLNIDVQSEASDTTEVSFSLSIRPCIEDKLGNSASQEQVTDFDVTASPKGKNDRAALNDREIWPCRINSREHGLPSKARDPQQEAKEELENLKKQHDLLKRMLKQQEQLKALQGRQAALLALQHKAEQAIAVMDDSVVTETTGSVSGVSLTSELNEELNDLIQRFHNQLHDSQTQSVPDNRRQAESLSLTREVSQSRNSSMSERLSDEKAQLFNKMRMLQGKKQKMDKLLGELHTLRDQHLNNSSFFPASGSPQRSIDQRSTTSAASGPAGIVTVVNGESNSLASAPYPPDSLVSQNESEEDDNLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDSVNENTKEEEETEESESDSEREDPQPVTNIRNPQGISSWSDINSNSNVQCGTNNRDGRHLNTDCEINNRSAANIRTLKMSSPLDCHNREDDKDIGLPQCEDEEVEEDRVSEDSASSHRSSLGDTAGDAEFEQKINRLIAAKQKLRQLQNLAAMVQDDDPAPQTTIANASNIGDLFLGEMEETKQQPNNVRASTNKLQKDVELNEKAREKFYEAKLQQQQQELKQLQEERRKLIEIQEKIQVLQKACPDLQLSAGLGDCTANRQTSPATSTPAMNDTAGKPSLEFDESVPVGNELWSEMRRHEILREELRQRRKQLEALMAEHQRRKELAETISTVAASVKSEGSEARRTPQQSRTENRTMATWGGSTQCALDEEDGDEDGYLSDGAGQAEEDDDDASSANDSFSVYPNNNVPENAYFVKENKDRWKNYRPLSADGNYRPLSKARQQQNISMRRQENLRWMSELSYVEEKERWQEQINQLKKQHEFSVSICQTLMQDQQTLSCLLQTLLTGPYSMMPNNVASSQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNDLVRQQEEQRYEEKSSRKDRGSGVPPPPSPVFCPFSFPPQPLNLFNVPGFTNFSSFAPDINYNPVFPSGFGDFAHNISPHSSEQQQQHPLDHNASGKTEYMAFPKPFESSSSNGAEKQRRSHRQPEEEMEKRSAWLNDSQDVKKDDQSQLKASFAVSVQNIASNHKNQSDMSRRREFDEESLESFSSMPDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKNKRKSSSQLKGRITNIDYESASASSVCEPCKSNKSRHSEEVGHAKVFSKRNREQLEKIIKYSRSTEMSSAHARRILQQSNRNACIEAPETGSDLSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHLSENNEKGKCTKSLNSAAWIASNSELTPSESLASTDDETFGKNFSTEACQDCEQNDADNGSSMSTSSNFEPFATDDLGNTVIHLDQALARMREYERMKIEAESTLDSEGCSSNFQGASAAKLEGGQSASECVPVPQSSEASAVPCPHIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSISVKQRCKRKIEATEVIQSYAKEAKKGLQVDVCSSAEDVDEDKDKDETETVKQVQDSEMYDGNRVPESIRSDASDQEEDDESESGPVAISLSKAETQALTNYGSGEDENEDEEIEFEEGPVDVQTSLQASSETTTENEQNSNQEQSKTKSSEILSSEQESVYAKGEQHVATILPYYLNVMENAPPLTVNTPESFITDSMKTEESSSSLPVNETQTLDTTCVGNKSAASSESSMAGSPDTESPVLVNEYEAGSGNVSQRSDEDDFVKVEDLPLKLAVYSEADLMKKMAMEAQTNSLSDELLGGGGAQDQELVGDAQTLKEPETFGVQSA

Target ORF information:

RefSeq Version	XM_021285854.1
Organism	Columba livia(rock pigeon)
Definition	Columba livia pericentriolar material 1 (PCM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021285854.1

ORF Insert Sequence:

ATGGCAACAG GAGGTGGTCC CTTTGAAGAA GGCATGAATG ATCAAGACTT GCCTAGCTGG 
AGCAATGACA GCTTTGATGA CCGGCTGAAC AACACGGACT GGGGAAGTCA ACAGAAGAAA 
GCAAACAGAT CTTCAGAAAA AAACAAGAAA AAGCTTGGTG GGGAAGCTGA AACAAGGCTT 
ACTAATGATA TATCTCCAGA ATCCTCACCT GGAATAGGAC GACGAAAGAC CAGAACTCCT 
CATAGTTTTC CTCATGCTCG ATACATGACC CAGATGTCTG TTCCGGAGCA GGCTGAGCTA 
GAAAGGCTTA AACAAAGAAT AAACTTCAGT GATCTGGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGCA GGGCAACAGC TGCTAACAAC AAACGTCAAC TTAATGAAAA CAAAAAACCA 
TTCAACTTCC TGTCACTGCA GATAAACACT AACAAGAGCA AAGATCCTGC CTCATGTTCC 
CAAAAAAAGG AAAGTGGGGT GTCAGTCCAA CGTAAAGAGT TGTTTGGTGC TGCTCTGAGC 
AAGGATTTCT TGCAAAATTG TCAAGTCTCT GCTCAAGAAG ATGGAGGGGG AGAACCAGCT 
ATGGATAGTA GCCAGATTGT GAGCAGACTA GTTCAAATTC GCGACTATAT TGCTAAGGCC 
AGCTCCATGC GGGATGATCT TGTAGAGAAA AATGAAAGAT CGGCCAATGT TGAGCGTTTA 
TCACACCTTA TAGATCACCT TAAAGAGCAG GAGAAATCCT ATCTGAAATT TTTGCAAAAG 
ATGCTTGCTA GACAAAATGA GGAGGATGAT GTTCGGACTA TAGATTCAGC TGTGGGATCT 
GGTTCTGTAG GTGAGAGCAC ATCGCTAAAC ATTGATGTGC AGTCTGAGGC TTCAGATACC 
ACGGAGGTAT CTTTTAGTTT GAGCATTCGG CCCTGCATTG AGGACAAACT AGGGAATTCA 
GCTTCACAAG AACAGGTTAC AGACTTTGAT GTTACTGCAA GCCCTAAAGG GAAAAATGAC 
AGAGCTGCCC TGAATGACAG GGAAATCTGG CCTTGTAGGA TTAATAGCCG AGAACATGGA 
TTGCCTTCAA AGGCTAGAGA TCCTCAACAG GAGGCTAAAG AGGAGTTGGA GAACTTGAAG 
AAGCAGCATG ATTTATTGAA AAGGATGCTA AAACAGCAAG AGCAATTAAA GGCTCTTCAA 
GGAAGACAGG CAGCTCTTCT TGCTTTGCAG CATAAAGCAG AGCAAGCAAT TGCTGTCATG 
GATGATTCTG TTGTAACAGA GACTACAGGT AGTGTTTCAG GAGTAAGCCT TACATCAGAA 
CTGAATGAAG AATTGAATGA CTTAATTCAG CGCTTTCACA ACCAACTTCA TGATTCTCAG 
ACACAGTCTG TACCAGACAA TAGAAGGCAA GCAGAAAGTC TTTCACTTAC TAGAGAGGTT 
TCACAAAGCA GAAACTCTTC AATGTCTGAA CGCCTGTCAG ATGAGAAGGC GCAGCTTTTT 
AACAAGATGC GAATGTTGCA GGGTAAAAAG CAAAAAATGG ACAAACTGTT AGGAGAACTT 
CATACACTTC GTGACCAACA TCTAAATAAC TCCTCCTTTT TTCCTGCTTC AGGTTCTCCT 
CAAAGAAGTA TTGATCAAAG AAGTACAACT TCAGCTGCTT CTGGTCCTGC AGGCATAGTA 
ACTGTTGTCA ATGGCGAATC AAATAGCCTG GCATCTGCTC CCTATCCTCC TGATTCCCTG 
GTTTCTCAGA ATGAGAGTGA AGAAGATGAC AATCTAAATC CAACAGAAAA GCTTCAGAAA 
CTAAATGAAG TTCGTAAGAG GCTGAATGAG TTACGTGAGC TAGTTCACTA CTATGAGCAA 
ACATCTGATA TGATGACAGA TTCTGTGAAT GAAAACACTA AGGAAGAAGA AGAAACCGAA 
GAATCAGAAA GTGATTCTGA ACGTGAGGAT CCACAGCCTG TTACAAATAT TAGAAATCCT 
CAAGGAATCA GTAGTTGGAG TGACATAAAT AGCAACTCAA ATGTACAGTG TGGAACTAAT 
AACAGAGATG GAAGACATCT TAATACAGAC TGTGAAATAA ATAACAGATC TGCTGCTAAC 
ATAAGGACTC TAAAAATGTC GTCACCTTTA GACTGTCATA ATAGGGAGGA TGACAAAGAT 
ATTGGCCTAC CCCAATGTGA AGATGAAGAA GTGGAAGAAG ATAGAGTTAG TGAAGATTCT 
GCATCTAGTC ACAGAAGCAG CCTGGGTGAT ACTGCTGGAG ATGCTGAGTT TGAGCAGAAG 
ATCAATAGGC TTATAGCTGC AAAACAGAAA CTTAGACAAT TACAAAACCT TGCTGCTATG 
GTGCAGGATG ATGATCCAGC ACCTCAGACA ACAATTGCAA ATGCATCTAA TATTGGTGAC 
TTGTTTTTGG GTGAGATGGA AGAGACAAAG CAGCAGCCAA ACAATGTCCG AGCTAGTACC 
AACAAATTAC AAAAAGATGT AGAACTGAAT GAAAAAGCAA GAGAGAAGTT TTATGAAGCT 
AAACTCCAGC AGCAGCAACA GGAGCTTAAG CAGTTACAAG AAGAAAGAAG AAAACTGATT 
GAAATTCAAG AAAAAATTCA GGTGTTACAG AAAGCTTGTC CTGATCTTCA ATTGTCAGCT 
GGCTTGGGTG ACTGCACAGC AAATAGACAG ACTTCACCAG CAACATCAAC TCCAGCCATG 
AATGACACAG CTGGCAAGCC TTCACTTGAA TTTGATGAAT CTGTACCAGT AGGCAATGAG 
TTATGGTCTG AGATGAGAAG ACATGAGATT TTAAGAGAAG AATTGCGACA AAGAAGAAAG 
CAACTGGAAG CTCTAATGGC TGAGCATCAG AGGAGGAAAG AGCTCGCAGA AACAATATCT 
ACTGTTGCTG CGTCTGTTAA AAGTGAAGGA TCAGAAGCTC GGCGTACTCC ACAGCAGAGT 
AGGACTGAAA ATAGGACAAT GGCTACCTGG GGAGGTTCTA CGCAGTGTGC ACTAGATGAA 
GAGGATGGAG ATGAAGATGG TTATCTCTCT GATGGAGCTG GTCAGGCAGA AGAAGATGAT 
GATGATGCGT CAAGTGCGAA TGACAGCTTC TCTGTTTATC CCAATAACAA CGTACCAGAA 
AATGCATATT TTGTTAAAGA AAACAAGGAT AGGTGGAAAA ATTACCGTCC TCTTTCAGCA 
GATGGAAATT ATCGTCCATT GTCTAAGGCC AGACAACAGC AAAACATAAG TATGCGACGT 
CAGGAAAACC TTCGGTGGAT GTCTGAACTT TCATATGTGG AAGAAAAGGA ACGATGGCAA 
GAGCAGATCA ATCAGTTGAA GAAACAGCAT GAATTTAGTG TCAGCATTTG TCAAACTTTA 
ATGCAGGATC AGCAGACCCT CTCTTGTCTT CTACAGACTT TGCTTACAGG CCCTTACAGT 
ATGATGCCCA ATAATGTTGC ATCTTCACAA GTACATCTTA TTATGCATCA GCTAAACCAG 
TGTTACACTC AACTGACATG GCAGCAGAAT AACGTCCAGA GGTTGAAACA AATGTTAAAT 
GATCTTGTGC GTCAGCAAGA AGAACAACGG TATGAAGAGA AGTCATCAAG AAAGGATAGA 
GGCAGTGGTG TACCCCCACC TCCATCTCCT GTTTTCTGTC CGTTCAGCTT TCCTCCACAA 
CCTTTGAACC TGTTTAATGT ACCAGGATTT ACTAATTTTT CTTCCTTTGC TCCAGATATT 
AACTATAATC CAGTGTTTCC TTCTGGTTTT GGAGATTTTG CACACAATAT TTCTCCACAC 
AGCAGTGAGC AGCAGCAGCA ACATCCTCTA GATCATAATG CATCTGGGAA AACTGAGTAT 
ATGGCGTTCC CCAAACCTTT TGAAAGCAGT TCCTCTAATG GAGCGGAAAA ACAAAGGAGG 
AGTCACAGAC AACCTGAAGA GGAAATGGAA AAAAGATCAG CTTGGCTTAA TGATAGCCAG 
GACGTGAAAA AAGATGATCA GTCTCAGCTG AAAGCAAGTT TTGCTGTTTC AGTACAAAAC 
ATTGCTTCCA ATCATAAAAA TCAGTCTGAT ATGAGCAGAA GAAGAGAGTT TGATGAAGAG 
TCTTTGGAGA GTTTTAGTAG CATGCCTGAT CCAGTAGACC CCACTACTGT GACAAAGACA 
TTTAAATCTA GAAAAGCATC AGCACAAGCA AGTTTGGCAT CAAAAGATAA AACACCTAAA 
TCGAAGAATA AGAGGAAGAG TTCTTCTCAG CTAAAAGGCA GAATTACAAA TATTGATTAT 
GAAAGTGCCA GTGCTTCTAG TGTGTGTGAA CCCTGCAAGA GCAATAAAAG CAGACACTCT 
GAAGAGGTTG GTCATGCAAA GGTGTTCAGC AAAAGGAATC GGGAACAACT GGAAAAAATC 
ATTAAATACA GTAGATCTAC AGAAATGTCT TCAGCGCATG CTAGGAGAAT TCTGCAGCAG 
TCTAACAGAA ATGCATGCAT TGAAGCGCCA GAAACTGGTA GTGATCTTTC TATGTTTGAA 
GCTTTACGAG ACACAATTTA TTCTGAAGTG GCAACTCTTA TTTCTCAAAA TGAGTCTCGT 
CCCCACTTTC TTATTGAACT TTTCCATGAG CTTCAGCTGC TAAATACAGA TTACCTGAGG 
CAAAGAGCTC TATACGCTCT ACAGGATATA GTGACCAGAC ATTTATCTGA GAACAATGAA 
AAAGGGAAGT GCACAAAATC ACTGAATTCT GCAGCATGGA TAGCATCAAA TTCTGAACTC 
ACTCCCAGTG AAAGCCTTGC TTCTACAGAT GATGAAACTT TTGGCAAAAA CTTTTCTACA 
GAAGCATGTC AAGATTGTGA ACAAAATGAT GCAGACAATG GGAGTAGCAT GTCTACATCT 
TCAAATTTTG AACCCTTTGC CACTGATGAC CTTGGCAACA CAGTGATTCA CTTAGATCAA 
GCTTTGGCTA GGATGAGGGA GTATGAGCGT ATGAAAATTG AAGCTGAAAG TACCCTTGAC 
TCTGAGGGCT GCTCTAGTAA TTTTCAGGGT GCTTCTGCTG CTAAATTAGA AGGAGGTCAA 
AGTGCCAGTG AATGTGTTCC TGTGCCACAG TCAAGTGAAG CTTCTGCTGT TCCATGTCCT 
CATATAGACA CTCAGCAGCT TGACCGGCAG ATTAAAGCAA TTATGAAAGA GGTCATTCCT 
TTTCTGAAGG AACACATGGA TGAAGTATGC TCTTCGCAGT TACTGACATC AGTAAGACGT 
ATGGTCTTGA CCCTTACACA ACAAAATGAT GAAAGTAAAG AGTTTGTGAA GTTCTTTCAT 
AAGCAGCTTG GCAGTATACT TCAGGATTCA CTGGCAAAAT TTGCTGGTAG AAAACTGAAA 
GATTGTGGAG AGGATCTTCT AGTGGAGATC TCTGAAGTGT TATTTAATGA ATTAGCCTTT 
TTTAAACTCA TGCAAGACTT GGACAACAAC AGTATTTCTG TAAAGCAGAG ATGTAAAAGA 
AAAATAGAAG CTACTGAAGT GATACAGTCG TATGCTAAAG AGGCAAAAAA AGGTCTCCAG 
GTGGATGTTT GTTCATCTGC TGAAGATGTC GATGAGGACA AAGACAAGGA TGAGACTGAA 
ACTGTTAAAC AAGTACAGGA CTCAGAAATG TATGATGGGA ATAGAGTTCC TGAAAGTATT 
AGGTCTGATG CATCTGATCA GGAGGAAGAT GATGAAAGTG AAAGCGGTCC AGTGGCAATA 
AGTTTATCAA AAGCAGAAAC CCAAGCTCTG ACTAACTATG GTAGTGGAGA AGATGAGAAT 
GAAGATGAAG AAATAGAATT TGAGGAAGGA CCTGTTGATG TGCAGACGTC ATTACAAGCC 
AGCAGTGAAA CAACAACTGA AAATGAACAG AATTCAAACC AAGAACAAAG TAAGACAAAA 
AGCAGTGAGA TTCTGTCATC AGAACAAGAA TCTGTTTATG CTAAAGGTGA ACAACATGTG 
GCTACAATTT TGCCTTATTA CCTCAATGTA ATGGAGAATG CACCGCCTTT AACGGTCAAT 
ACCCCAGAAT CTTTTATAAC AGACAGTATG AAAACAGAAG AATCAAGTTC ATCTTTACCA 
GTAAATGAAA CTCAAACACT GGATACAACG TGTGTAGGAA ACAAATCTGC TGCAAGTTCT 
GAAAGCTCCA TGGCTGGCAG CCCTGATACA GAGTCACCTG TGTTAGTGAA TGAATATGAA 
GCTGGTTCTG GAAATGTAAG TCAAAGATCT GATGAAGATG ACTTTGTGAA AGTTGAAGAC 
TTGCCCCTTA AACTTGCAGT GTATTCAGAG GCAGATTTAA TGAAGAAAAT GGCAATGGAG 
GCCCAAACGA ACAGTTTGTC TGATGAATTA CTGGGTGGAG GTGGAGCTCA AGATCAAGAA 
TTAGTAGGAG ATGCCCAAAC TTTGAAAGAA CCTGAAACTT TTGGAGTTCA AAGTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_021285857.1 ) cDNA ORF clone, Columba livia(rock pigeon) -> XP_021141532.1 Columba livia pericentriolar material 1 (PCM1), transcript variant X4, mRNA.

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CloneID	OCo293160
Clone ID Related Accession (Same CDS sequence)	XM_021285854.1 , XM_021285857.1 , XM_021285856.1 , XM_021285855.1
Accession Version	XM_021285857.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-23
Organism	Columba livia(rock pigeon)
Product	pericentriolar material 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004973253.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Columba livia Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACAG GAGGTGGTCC CTTTGAAGAA GGCATGAATG ATCAAGACTT GCCTAGCTGG 
AGCAATGACA GCTTTGATGA CCGGCTGAAC AACACGGACT GGGGAAGTCA ACAGAAGAAA 
GCAAACAGAT CTTCAGAAAA AAACAAGAAA AAGCTTGGTG GGGAAGCTGA AACAAGGCTT 
ACTAATGATA TATCTCCAGA ATCCTCACCT GGAATAGGAC GACGAAAGAC CAGAACTCCT 
CATAGTTTTC CTCATGCTCG ATACATGACC CAGATGTCTG TTCCGGAGCA GGCTGAGCTA 
GAAAGGCTTA AACAAAGAAT AAACTTCAGT GATCTGGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGCA GGGCAACAGC TGCTAACAAC AAACGTCAAC TTAATGAAAA CAAAAAACCA 
TTCAACTTCC TGTCACTGCA GATAAACACT AACAAGAGCA AAGATCCTGC CTCATGTTCC 
CAAAAAAAGG AAAGTGGGGT GTCAGTCCAA CGTAAAGAGT TGTTTGGTGC TGCTCTGAGC 
AAGGATTTCT TGCAAAATTG TCAAGTCTCT GCTCAAGAAG ATGGAGGGGG AGAACCAGCT 
ATGGATAGTA GCCAGATTGT GAGCAGACTA GTTCAAATTC GCGACTATAT TGCTAAGGCC 
AGCTCCATGC GGGATGATCT TGTAGAGAAA AATGAAAGAT CGGCCAATGT TGAGCGTTTA 
TCACACCTTA TAGATCACCT TAAAGAGCAG GAGAAATCCT ATCTGAAATT TTTGCAAAAG 
ATGCTTGCTA GACAAAATGA GGAGGATGAT GTTCGGACTA TAGATTCAGC TGTGGGATCT 
GGTTCTGTAG GTGAGAGCAC ATCGCTAAAC ATTGATGTGC AGTCTGAGGC TTCAGATACC 
ACGGAGGTAT CTTTTAGTTT GAGCATTCGG CCCTGCATTG AGGACAAACT AGGGAATTCA 
GCTTCACAAG AACAGGTTAC AGACTTTGAT GTTACTGCAA GCCCTAAAGG GAAAAATGAC 
AGAGCTGCCC TGAATGACAG GGAAATCTGG CCTTGTAGGA TTAATAGCCG AGAACATGGA 
TTGCCTTCAA AGGCTAGAGA TCCTCAACAG GAGGCTAAAG AGGAGTTGGA GAACTTGAAG 
AAGCAGCATG ATTTATTGAA AAGGATGCTA AAACAGCAAG AGCAATTAAA GGCTCTTCAA 
GGAAGACAGG CAGCTCTTCT TGCTTTGCAG CATAAAGCAG AGCAAGCAAT TGCTGTCATG 
GATGATTCTG TTGTAACAGA GACTACAGGT AGTGTTTCAG GAGTAAGCCT TACATCAGAA 
CTGAATGAAG AATTGAATGA CTTAATTCAG CGCTTTCACA ACCAACTTCA TGATTCTCAG 
ACACAGTCTG TACCAGACAA TAGAAGGCAA GCAGAAAGTC TTTCACTTAC TAGAGAGGTT 
TCACAAAGCA GAAACTCTTC AATGTCTGAA CGCCTGTCAG ATGAGAAGGC GCAGCTTTTT 
AACAAGATGC GAATGTTGCA GGGTAAAAAG CAAAAAATGG ACAAACTGTT AGGAGAACTT 
CATACACTTC GTGACCAACA TCTAAATAAC TCCTCCTTTT TTCCTGCTTC AGGTTCTCCT 
CAAAGAAGTA TTGATCAAAG AAGTACAACT TCAGCTGCTT CTGGTCCTGC AGGCATAGTA 
ACTGTTGTCA ATGGCGAATC AAATAGCCTG GCATCTGCTC CCTATCCTCC TGATTCCCTG 
GTTTCTCAGA ATGAGAGTGA AGAAGATGAC AATCTAAATC CAACAGAAAA GCTTCAGAAA 
CTAAATGAAG TTCGTAAGAG GCTGAATGAG TTACGTGAGC TAGTTCACTA CTATGAGCAA 
ACATCTGATA TGATGACAGA TTCTGTGAAT GAAAACACTA AGGAAGAAGA AGAAACCGAA 
GAATCAGAAA GTGATTCTGA ACGTGAGGAT CCACAGCCTG TTACAAATAT TAGAAATCCT 
CAAGGAATCA GTAGTTGGAG TGACATAAAT AGCAACTCAA ATGTACAGTG TGGAACTAAT 
AACAGAGATG GAAGACATCT TAATACAGAC TGTGAAATAA ATAACAGATC TGCTGCTAAC 
ATAAGGACTC TAAAAATGTC GTCACCTTTA GACTGTCATA ATAGGGAGGA TGACAAAGAT 
ATTGGCCTAC CCCAATGTGA AGATGAAGAA GTGGAAGAAG ATAGAGTTAG TGAAGATTCT 
GCATCTAGTC ACAGAAGCAG CCTGGGTGAT ACTGCTGGAG ATGCTGAGTT TGAGCAGAAG 
ATCAATAGGC TTATAGCTGC AAAACAGAAA CTTAGACAAT TACAAAACCT TGCTGCTATG 
GTGCAGGATG ATGATCCAGC ACCTCAGACA ACAATTGCAA ATGCATCTAA TATTGGTGAC 
TTGTTTTTGG GTGAGATGGA AGAGACAAAG CAGCAGCCAA ACAATGTCCG AGCTAGTACC 
AACAAATTAC AAAAAGATGT AGAACTGAAT GAAAAAGCAA GAGAGAAGTT TTATGAAGCT 
AAACTCCAGC AGCAGCAACA GGAGCTTAAG CAGTTACAAG AAGAAAGAAG AAAACTGATT 
GAAATTCAAG AAAAAATTCA GGTGTTACAG AAAGCTTGTC CTGATCTTCA ATTGTCAGCT 
GGCTTGGGTG ACTGCACAGC AAATAGACAG ACTTCACCAG CAACATCAAC TCCAGCCATG 
AATGACACAG CTGGCAAGCC TTCACTTGAA TTTGATGAAT CTGTACCAGT AGGCAATGAG 
TTATGGTCTG AGATGAGAAG ACATGAGATT TTAAGAGAAG AATTGCGACA AAGAAGAAAG 
CAACTGGAAG CTCTAATGGC TGAGCATCAG AGGAGGAAAG AGCTCGCAGA AACAATATCT 
ACTGTTGCTG CGTCTGTTAA AAGTGAAGGA TCAGAAGCTC GGCGTACTCC ACAGCAGAGT 
AGGACTGAAA ATAGGACAAT GGCTACCTGG GGAGGTTCTA CGCAGTGTGC ACTAGATGAA 
GAGGATGGAG ATGAAGATGG TTATCTCTCT GATGGAGCTG GTCAGGCAGA AGAAGATGAT 
GATGATGCGT CAAGTGCGAA TGACAGCTTC TCTGTTTATC CCAATAACAA CGTACCAGAA 
AATGCATATT TTGTTAAAGA AAACAAGGAT AGGTGGAAAA ATTACCGTCC TCTTTCAGCA 
GATGGAAATT ATCGTCCATT GTCTAAGGCC AGACAACAGC AAAACATAAG TATGCGACGT 
CAGGAAAACC TTCGGTGGAT GTCTGAACTT TCATATGTGG AAGAAAAGGA ACGATGGCAA 
GAGCAGATCA ATCAGTTGAA GAAACAGCAT GAATTTAGTG TCAGCATTTG TCAAACTTTA 
ATGCAGGATC AGCAGACCCT CTCTTGTCTT CTACAGACTT TGCTTACAGG CCCTTACAGT 
ATGATGCCCA ATAATGTTGC ATCTTCACAA GTACATCTTA TTATGCATCA GCTAAACCAG 
TGTTACACTC AACTGACATG GCAGCAGAAT AACGTCCAGA GGTTGAAACA AATGTTAAAT 
GATCTTGTGC GTCAGCAAGA AGAACAACGG TATGAAGAGA AGTCATCAAG AAAGGATAGA 
GGCAGTGGTG TACCCCCACC TCCATCTCCT GTTTTCTGTC CGTTCAGCTT TCCTCCACAA 
CCTTTGAACC TGTTTAATGT ACCAGGATTT ACTAATTTTT CTTCCTTTGC TCCAGATATT 
AACTATAATC CAGTGTTTCC TTCTGGTTTT GGAGATTTTG CACACAATAT TTCTCCACAC 
AGCAGTGAGC AGCAGCAGCA ACATCCTCTA GATCATAATG CATCTGGGAA AACTGAGTAT 
ATGGCGTTCC CCAAACCTTT TGAAAGCAGT TCCTCTAATG GAGCGGAAAA ACAAAGGAGG 
AGTCACAGAC AACCTGAAGA GGAAATGGAA AAAAGATCAG CTTGGCTTAA TGATAGCCAG 
GACGTGAAAA AAGATGATCA GTCTCAGCTG AAAGCAAGTT TTGCTGTTTC AGTACAAAAC 
ATTGCTTCCA ATCATAAAAA TCAGTCTGAT ATGAGCAGAA GAAGAGAGTT TGATGAAGAG 
TCTTTGGAGA GTTTTAGTAG CATGCCTGAT CCAGTAGACC CCACTACTGT GACAAAGACA 
TTTAAATCTA GAAAAGCATC AGCACAAGCA AGTTTGGCAT CAAAAGATAA AACACCTAAA 
TCGAAGAATA AGAGGAAGAG TTCTTCTCAG CTAAAAGGCA GAATTACAAA TATTGATTAT 
GAAAGTGCCA GTGCTTCTAG TGTGTGTGAA CCCTGCAAGA GCAATAAAAG CAGACACTCT 
GAAGAGGTTG GTCATGCAAA GGTGTTCAGC AAAAGGAATC GGGAACAACT GGAAAAAATC 
ATTAAATACA GTAGATCTAC AGAAATGTCT TCAGCGCATG CTAGGAGAAT TCTGCAGCAG 
TCTAACAGAA ATGCATGCAT TGAAGCGCCA GAAACTGGTA GTGATCTTTC TATGTTTGAA 
GCTTTACGAG ACACAATTTA TTCTGAAGTG GCAACTCTTA TTTCTCAAAA TGAGTCTCGT 
CCCCACTTTC TTATTGAACT TTTCCATGAG CTTCAGCTGC TAAATACAGA TTACCTGAGG 
CAAAGAGCTC TATACGCTCT ACAGGATATA GTGACCAGAC ATTTATCTGA GAACAATGAA 
AAAGGGAAGT GCACAAAATC ACTGAATTCT GCAGCATGGA TAGCATCAAA TTCTGAACTC 
ACTCCCAGTG AAAGCCTTGC TTCTACAGAT GATGAAACTT TTGGCAAAAA CTTTTCTACA 
GAAGCATGTC AAGATTGTGA ACAAAATGAT GCAGACAATG GGAGTAGCAT GTCTACATCT 
TCAAATTTTG AACCCTTTGC CACTGATGAC CTTGGCAACA CAGTGATTCA CTTAGATCAA 
GCTTTGGCTA GGATGAGGGA GTATGAGCGT ATGAAAATTG AAGCTGAAAG TACCCTTGAC 
TCTGAGGGCT GCTCTAGTAA TTTTCAGGGT GCTTCTGCTG CTAAATTAGA AGGAGGTCAA 
AGTGCCAGTG AATGTGTTCC TGTGCCACAG TCAAGTGAAG CTTCTGCTGT TCCATGTCCT 
CATATAGACA CTCAGCAGCT TGACCGGCAG ATTAAAGCAA TTATGAAAGA GGTCATTCCT 
TTTCTGAAGG AACACATGGA TGAAGTATGC TCTTCGCAGT TACTGACATC AGTAAGACGT 
ATGGTCTTGA CCCTTACACA ACAAAATGAT GAAAGTAAAG AGTTTGTGAA GTTCTTTCAT 
AAGCAGCTTG GCAGTATACT TCAGGATTCA CTGGCAAAAT TTGCTGGTAG AAAACTGAAA 
GATTGTGGAG AGGATCTTCT AGTGGAGATC TCTGAAGTGT TATTTAATGA ATTAGCCTTT 
TTTAAACTCA TGCAAGACTT GGACAACAAC AGTATTTCTG TAAAGCAGAG ATGTAAAAGA 
AAAATAGAAG CTACTGAAGT GATACAGTCG TATGCTAAAG AGGCAAAAAA AGGTCTCCAG 
GTGGATGTTT GTTCATCTGC TGAAGATGTC GATGAGGACA AAGACAAGGA TGAGACTGAA 
ACTGTTAAAC AAGTACAGGA CTCAGAAATG TATGATGGGA ATAGAGTTCC TGAAAGTATT 
AGGTCTGATG CATCTGATCA GGAGGAAGAT GATGAAAGTG AAAGCGGTCC AGTGGCAATA 
AGTTTATCAA AAGCAGAAAC CCAAGCTCTG ACTAACTATG GTAGTGGAGA AGATGAGAAT 
GAAGATGAAG AAATAGAATT TGAGGAAGGA CCTGTTGATG TGCAGACGTC ATTACAAGCC 
AGCAGTGAAA CAACAACTGA AAATGAACAG AATTCAAACC AAGAACAAAG TAAGACAAAA 
AGCAGTGAGA TTCTGTCATC AGAACAAGAA TCTGTTTATG CTAAAGGTGA ACAACATGTG 
GCTACAATTT TGCCTTATTA CCTCAATGTA ATGGAGAATG CACCGCCTTT AACGGTCAAT 
ACCCCAGAAT CTTTTATAAC AGACAGTATG AAAACAGAAG AATCAAGTTC ATCTTTACCA 
GTAAATGAAA CTCAAACACT GGATACAACG TGTGTAGGAA ACAAATCTGC TGCAAGTTCT 
GAAAGCTCCA TGGCTGGCAG CCCTGATACA GAGTCACCTG TGTTAGTGAA TGAATATGAA 
GCTGGTTCTG GAAATGTAAG TCAAAGATCT GATGAAGATG ACTTTGTGAA AGTTGAAGAC 
TTGCCCCTTA AACTTGCAGT GTATTCAGAG GCAGATTTAA TGAAGAAAAT GGCAATGGAG 
GCCCAAACGA ACAGTTTGTC TGATGAATTA CTGGGTGGAG GTGGAGCTCA AGATCAAGAA 
TTAGTAGGAG ATGCCCAAAC TTTGAAAGAA CCTGAAACTT TTGGAGTTCA AAGTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021141532.1
CDS	59..6478
Translation	MATGGGPFEEGMNDQDLPSWSNDSFDDRLNNTDWGSQQKKANRSSEKNKKKLGGEAETRLTNDISPESSPGIGRRKTRTPHSFPHARYMTQMSVPEQAELERLKQRINFSDLDQRSIGSDSQGRATAANNKRQLNENKKPFNFLSLQINTNKSKDPASCSQKKESGVSVQRKELFGAALSKDFLQNCQVSAQEDGGGEPAMDSSQIVSRLVQIRDYIAKASSMRDDLVEKNERSANVERLSHLIDHLKEQEKSYLKFLQKMLARQNEEDDVRTIDSAVGSGSVGESTSLNIDVQSEASDTTEVSFSLSIRPCIEDKLGNSASQEQVTDFDVTASPKGKNDRAALNDREIWPCRINSREHGLPSKARDPQQEAKEELENLKKQHDLLKRMLKQQEQLKALQGRQAALLALQHKAEQAIAVMDDSVVTETTGSVSGVSLTSELNEELNDLIQRFHNQLHDSQTQSVPDNRRQAESLSLTREVSQSRNSSMSERLSDEKAQLFNKMRMLQGKKQKMDKLLGELHTLRDQHLNNSSFFPASGSPQRSIDQRSTTSAASGPAGIVTVVNGESNSLASAPYPPDSLVSQNESEEDDNLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDSVNENTKEEEETEESESDSEREDPQPVTNIRNPQGISSWSDINSNSNVQCGTNNRDGRHLNTDCEINNRSAANIRTLKMSSPLDCHNREDDKDIGLPQCEDEEVEEDRVSEDSASSHRSSLGDTAGDAEFEQKINRLIAAKQKLRQLQNLAAMVQDDDPAPQTTIANASNIGDLFLGEMEETKQQPNNVRASTNKLQKDVELNEKAREKFYEAKLQQQQQELKQLQEERRKLIEIQEKIQVLQKACPDLQLSAGLGDCTANRQTSPATSTPAMNDTAGKPSLEFDESVPVGNELWSEMRRHEILREELRQRRKQLEALMAEHQRRKELAETISTVAASVKSEGSEARRTPQQSRTENRTMATWGGSTQCALDEEDGDEDGYLSDGAGQAEEDDDDASSANDSFSVYPNNNVPENAYFVKENKDRWKNYRPLSADGNYRPLSKARQQQNISMRRQENLRWMSELSYVEEKERWQEQINQLKKQHEFSVSICQTLMQDQQTLSCLLQTLLTGPYSMMPNNVASSQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNDLVRQQEEQRYEEKSSRKDRGSGVPPPPSPVFCPFSFPPQPLNLFNVPGFTNFSSFAPDINYNPVFPSGFGDFAHNISPHSSEQQQQHPLDHNASGKTEYMAFPKPFESSSSNGAEKQRRSHRQPEEEMEKRSAWLNDSQDVKKDDQSQLKASFAVSVQNIASNHKNQSDMSRRREFDEESLESFSSMPDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKNKRKSSSQLKGRITNIDYESASASSVCEPCKSNKSRHSEEVGHAKVFSKRNREQLEKIIKYSRSTEMSSAHARRILQQSNRNACIEAPETGSDLSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHLSENNEKGKCTKSLNSAAWIASNSELTPSESLASTDDETFGKNFSTEACQDCEQNDADNGSSMSTSSNFEPFATDDLGNTVIHLDQALARMREYERMKIEAESTLDSEGCSSNFQGASAAKLEGGQSASECVPVPQSSEASAVPCPHIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSISVKQRCKRKIEATEVIQSYAKEAKKGLQVDVCSSAEDVDEDKDKDETETVKQVQDSEMYDGNRVPESIRSDASDQEEDDESESGPVAISLSKAETQALTNYGSGEDENEDEEIEFEEGPVDVQTSLQASSETTTENEQNSNQEQSKTKSSEILSSEQESVYAKGEQHVATILPYYLNVMENAPPLTVNTPESFITDSMKTEESSSSLPVNETQTLDTTCVGNKSAASSESSMAGSPDTESPVLVNEYEAGSGNVSQRSDEDDFVKVEDLPLKLAVYSEADLMKKMAMEAQTNSLSDELLGGGGAQDQELVGDAQTLKEPETFGVQSA

Target ORF information:

RefSeq Version	XM_021285857.1
Organism	Columba livia(rock pigeon)
Definition	Columba livia pericentriolar material 1 (PCM1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021285857.1

ORF Insert Sequence:

ATGGCAACAG GAGGTGGTCC CTTTGAAGAA GGCATGAATG ATCAAGACTT GCCTAGCTGG 
AGCAATGACA GCTTTGATGA CCGGCTGAAC AACACGGACT GGGGAAGTCA ACAGAAGAAA 
GCAAACAGAT CTTCAGAAAA AAACAAGAAA AAGCTTGGTG GGGAAGCTGA AACAAGGCTT 
ACTAATGATA TATCTCCAGA ATCCTCACCT GGAATAGGAC GACGAAAGAC CAGAACTCCT 
CATAGTTTTC CTCATGCTCG ATACATGACC CAGATGTCTG TTCCGGAGCA GGCTGAGCTA 
GAAAGGCTTA AACAAAGAAT AAACTTCAGT GATCTGGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGCA GGGCAACAGC TGCTAACAAC AAACGTCAAC TTAATGAAAA CAAAAAACCA 
TTCAACTTCC TGTCACTGCA GATAAACACT AACAAGAGCA AAGATCCTGC CTCATGTTCC 
CAAAAAAAGG AAAGTGGGGT GTCAGTCCAA CGTAAAGAGT TGTTTGGTGC TGCTCTGAGC 
AAGGATTTCT TGCAAAATTG TCAAGTCTCT GCTCAAGAAG ATGGAGGGGG AGAACCAGCT 
ATGGATAGTA GCCAGATTGT GAGCAGACTA GTTCAAATTC GCGACTATAT TGCTAAGGCC 
AGCTCCATGC GGGATGATCT TGTAGAGAAA AATGAAAGAT CGGCCAATGT TGAGCGTTTA 
TCACACCTTA TAGATCACCT TAAAGAGCAG GAGAAATCCT ATCTGAAATT TTTGCAAAAG 
ATGCTTGCTA GACAAAATGA GGAGGATGAT GTTCGGACTA TAGATTCAGC TGTGGGATCT 
GGTTCTGTAG GTGAGAGCAC ATCGCTAAAC ATTGATGTGC AGTCTGAGGC TTCAGATACC 
ACGGAGGTAT CTTTTAGTTT GAGCATTCGG CCCTGCATTG AGGACAAACT AGGGAATTCA 
GCTTCACAAG AACAGGTTAC AGACTTTGAT GTTACTGCAA GCCCTAAAGG GAAAAATGAC 
AGAGCTGCCC TGAATGACAG GGAAATCTGG CCTTGTAGGA TTAATAGCCG AGAACATGGA 
TTGCCTTCAA AGGCTAGAGA TCCTCAACAG GAGGCTAAAG AGGAGTTGGA GAACTTGAAG 
AAGCAGCATG ATTTATTGAA AAGGATGCTA AAACAGCAAG AGCAATTAAA GGCTCTTCAA 
GGAAGACAGG CAGCTCTTCT TGCTTTGCAG CATAAAGCAG AGCAAGCAAT TGCTGTCATG 
GATGATTCTG TTGTAACAGA GACTACAGGT AGTGTTTCAG GAGTAAGCCT TACATCAGAA 
CTGAATGAAG AATTGAATGA CTTAATTCAG CGCTTTCACA ACCAACTTCA TGATTCTCAG 
ACACAGTCTG TACCAGACAA TAGAAGGCAA GCAGAAAGTC TTTCACTTAC TAGAGAGGTT 
TCACAAAGCA GAAACTCTTC AATGTCTGAA CGCCTGTCAG ATGAGAAGGC GCAGCTTTTT 
AACAAGATGC GAATGTTGCA GGGTAAAAAG CAAAAAATGG ACAAACTGTT AGGAGAACTT 
CATACACTTC GTGACCAACA TCTAAATAAC TCCTCCTTTT TTCCTGCTTC AGGTTCTCCT 
CAAAGAAGTA TTGATCAAAG AAGTACAACT TCAGCTGCTT CTGGTCCTGC AGGCATAGTA 
ACTGTTGTCA ATGGCGAATC AAATAGCCTG GCATCTGCTC CCTATCCTCC TGATTCCCTG 
GTTTCTCAGA ATGAGAGTGA AGAAGATGAC AATCTAAATC CAACAGAAAA GCTTCAGAAA 
CTAAATGAAG TTCGTAAGAG GCTGAATGAG TTACGTGAGC TAGTTCACTA CTATGAGCAA 
ACATCTGATA TGATGACAGA TTCTGTGAAT GAAAACACTA AGGAAGAAGA AGAAACCGAA 
GAATCAGAAA GTGATTCTGA ACGTGAGGAT CCACAGCCTG TTACAAATAT TAGAAATCCT 
CAAGGAATCA GTAGTTGGAG TGACATAAAT AGCAACTCAA ATGTACAGTG TGGAACTAAT 
AACAGAGATG GAAGACATCT TAATACAGAC TGTGAAATAA ATAACAGATC TGCTGCTAAC 
ATAAGGACTC TAAAAATGTC GTCACCTTTA GACTGTCATA ATAGGGAGGA TGACAAAGAT 
ATTGGCCTAC CCCAATGTGA AGATGAAGAA GTGGAAGAAG ATAGAGTTAG TGAAGATTCT 
GCATCTAGTC ACAGAAGCAG CCTGGGTGAT ACTGCTGGAG ATGCTGAGTT TGAGCAGAAG 
ATCAATAGGC TTATAGCTGC AAAACAGAAA CTTAGACAAT TACAAAACCT TGCTGCTATG 
GTGCAGGATG ATGATCCAGC ACCTCAGACA ACAATTGCAA ATGCATCTAA TATTGGTGAC 
TTGTTTTTGG GTGAGATGGA AGAGACAAAG CAGCAGCCAA ACAATGTCCG AGCTAGTACC 
AACAAATTAC AAAAAGATGT AGAACTGAAT GAAAAAGCAA GAGAGAAGTT TTATGAAGCT 
AAACTCCAGC AGCAGCAACA GGAGCTTAAG CAGTTACAAG AAGAAAGAAG AAAACTGATT 
GAAATTCAAG AAAAAATTCA GGTGTTACAG AAAGCTTGTC CTGATCTTCA ATTGTCAGCT 
GGCTTGGGTG ACTGCACAGC AAATAGACAG ACTTCACCAG CAACATCAAC TCCAGCCATG 
AATGACACAG CTGGCAAGCC TTCACTTGAA TTTGATGAAT CTGTACCAGT AGGCAATGAG 
TTATGGTCTG AGATGAGAAG ACATGAGATT TTAAGAGAAG AATTGCGACA AAGAAGAAAG 
CAACTGGAAG CTCTAATGGC TGAGCATCAG AGGAGGAAAG AGCTCGCAGA AACAATATCT 
ACTGTTGCTG CGTCTGTTAA AAGTGAAGGA TCAGAAGCTC GGCGTACTCC ACAGCAGAGT 
AGGACTGAAA ATAGGACAAT GGCTACCTGG GGAGGTTCTA CGCAGTGTGC ACTAGATGAA 
GAGGATGGAG ATGAAGATGG TTATCTCTCT GATGGAGCTG GTCAGGCAGA AGAAGATGAT 
GATGATGCGT CAAGTGCGAA TGACAGCTTC TCTGTTTATC CCAATAACAA CGTACCAGAA 
AATGCATATT TTGTTAAAGA AAACAAGGAT AGGTGGAAAA ATTACCGTCC TCTTTCAGCA 
GATGGAAATT ATCGTCCATT GTCTAAGGCC AGACAACAGC AAAACATAAG TATGCGACGT 
CAGGAAAACC TTCGGTGGAT GTCTGAACTT TCATATGTGG AAGAAAAGGA ACGATGGCAA 
GAGCAGATCA ATCAGTTGAA GAAACAGCAT GAATTTAGTG TCAGCATTTG TCAAACTTTA 
ATGCAGGATC AGCAGACCCT CTCTTGTCTT CTACAGACTT TGCTTACAGG CCCTTACAGT 
ATGATGCCCA ATAATGTTGC ATCTTCACAA GTACATCTTA TTATGCATCA GCTAAACCAG 
TGTTACACTC AACTGACATG GCAGCAGAAT AACGTCCAGA GGTTGAAACA AATGTTAAAT 
GATCTTGTGC GTCAGCAAGA AGAACAACGG TATGAAGAGA AGTCATCAAG AAAGGATAGA 
GGCAGTGGTG TACCCCCACC TCCATCTCCT GTTTTCTGTC CGTTCAGCTT TCCTCCACAA 
CCTTTGAACC TGTTTAATGT ACCAGGATTT ACTAATTTTT CTTCCTTTGC TCCAGATATT 
AACTATAATC CAGTGTTTCC TTCTGGTTTT GGAGATTTTG CACACAATAT TTCTCCACAC 
AGCAGTGAGC AGCAGCAGCA ACATCCTCTA GATCATAATG CATCTGGGAA AACTGAGTAT 
ATGGCGTTCC CCAAACCTTT TGAAAGCAGT TCCTCTAATG GAGCGGAAAA ACAAAGGAGG 
AGTCACAGAC AACCTGAAGA GGAAATGGAA AAAAGATCAG CTTGGCTTAA TGATAGCCAG 
GACGTGAAAA AAGATGATCA GTCTCAGCTG AAAGCAAGTT TTGCTGTTTC AGTACAAAAC 
ATTGCTTCCA ATCATAAAAA TCAGTCTGAT ATGAGCAGAA GAAGAGAGTT TGATGAAGAG 
TCTTTGGAGA GTTTTAGTAG CATGCCTGAT CCAGTAGACC CCACTACTGT GACAAAGACA 
TTTAAATCTA GAAAAGCATC AGCACAAGCA AGTTTGGCAT CAAAAGATAA AACACCTAAA 
TCGAAGAATA AGAGGAAGAG TTCTTCTCAG CTAAAAGGCA GAATTACAAA TATTGATTAT 
GAAAGTGCCA GTGCTTCTAG TGTGTGTGAA CCCTGCAAGA GCAATAAAAG CAGACACTCT 
GAAGAGGTTG GTCATGCAAA GGTGTTCAGC AAAAGGAATC GGGAACAACT GGAAAAAATC 
ATTAAATACA GTAGATCTAC AGAAATGTCT TCAGCGCATG CTAGGAGAAT TCTGCAGCAG 
TCTAACAGAA ATGCATGCAT TGAAGCGCCA GAAACTGGTA GTGATCTTTC TATGTTTGAA 
GCTTTACGAG ACACAATTTA TTCTGAAGTG GCAACTCTTA TTTCTCAAAA TGAGTCTCGT 
CCCCACTTTC TTATTGAACT TTTCCATGAG CTTCAGCTGC TAAATACAGA TTACCTGAGG 
CAAAGAGCTC TATACGCTCT ACAGGATATA GTGACCAGAC ATTTATCTGA GAACAATGAA 
AAAGGGAAGT GCACAAAATC ACTGAATTCT GCAGCATGGA TAGCATCAAA TTCTGAACTC 
ACTCCCAGTG AAAGCCTTGC TTCTACAGAT GATGAAACTT TTGGCAAAAA CTTTTCTACA 
GAAGCATGTC AAGATTGTGA ACAAAATGAT GCAGACAATG GGAGTAGCAT GTCTACATCT 
TCAAATTTTG AACCCTTTGC CACTGATGAC CTTGGCAACA CAGTGATTCA CTTAGATCAA 
GCTTTGGCTA GGATGAGGGA GTATGAGCGT ATGAAAATTG AAGCTGAAAG TACCCTTGAC 
TCTGAGGGCT GCTCTAGTAA TTTTCAGGGT GCTTCTGCTG CTAAATTAGA AGGAGGTCAA 
AGTGCCAGTG AATGTGTTCC TGTGCCACAG TCAAGTGAAG CTTCTGCTGT TCCATGTCCT 
CATATAGACA CTCAGCAGCT TGACCGGCAG ATTAAAGCAA TTATGAAAGA GGTCATTCCT 
TTTCTGAAGG AACACATGGA TGAAGTATGC TCTTCGCAGT TACTGACATC AGTAAGACGT 
ATGGTCTTGA CCCTTACACA ACAAAATGAT GAAAGTAAAG AGTTTGTGAA GTTCTTTCAT 
AAGCAGCTTG GCAGTATACT TCAGGATTCA CTGGCAAAAT TTGCTGGTAG AAAACTGAAA 
GATTGTGGAG AGGATCTTCT AGTGGAGATC TCTGAAGTGT TATTTAATGA ATTAGCCTTT 
TTTAAACTCA TGCAAGACTT GGACAACAAC AGTATTTCTG TAAAGCAGAG ATGTAAAAGA 
AAAATAGAAG CTACTGAAGT GATACAGTCG TATGCTAAAG AGGCAAAAAA AGGTCTCCAG 
GTGGATGTTT GTTCATCTGC TGAAGATGTC GATGAGGACA AAGACAAGGA TGAGACTGAA 
ACTGTTAAAC AAGTACAGGA CTCAGAAATG TATGATGGGA ATAGAGTTCC TGAAAGTATT 
AGGTCTGATG CATCTGATCA GGAGGAAGAT GATGAAAGTG AAAGCGGTCC AGTGGCAATA 
AGTTTATCAA AAGCAGAAAC CCAAGCTCTG ACTAACTATG GTAGTGGAGA AGATGAGAAT 
GAAGATGAAG AAATAGAATT TGAGGAAGGA CCTGTTGATG TGCAGACGTC ATTACAAGCC 
AGCAGTGAAA CAACAACTGA AAATGAACAG AATTCAAACC AAGAACAAAG TAAGACAAAA 
AGCAGTGAGA TTCTGTCATC AGAACAAGAA TCTGTTTATG CTAAAGGTGA ACAACATGTG 
GCTACAATTT TGCCTTATTA CCTCAATGTA ATGGAGAATG CACCGCCTTT AACGGTCAAT 
ACCCCAGAAT CTTTTATAAC AGACAGTATG AAAACAGAAG AATCAAGTTC ATCTTTACCA 
GTAAATGAAA CTCAAACACT GGATACAACG TGTGTAGGAA ACAAATCTGC TGCAAGTTCT 
GAAAGCTCCA TGGCTGGCAG CCCTGATACA GAGTCACCTG TGTTAGTGAA TGAATATGAA 
GCTGGTTCTG GAAATGTAAG TCAAAGATCT GATGAAGATG ACTTTGTGAA AGTTGAAGAC 
TTGCCCCTTA AACTTGCAGT GTATTCAGAG GCAGATTTAA TGAAGAAAAT GGCAATGGAG 
GCCCAAACGA ACAGTTTGTC TGATGAATTA CTGGGTGGAG GTGGAGCTCA AGATCAAGAA 
TTAGTAGGAG ATGCCCAAAC TTTGAAAGAA CCTGAAACTT TTGGAGTTCA AAGTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_021285856.1 ) cDNA ORF clone, Columba livia(rock pigeon) -> XP_021141531.1 Columba livia pericentriolar material 1 (PCM1), transcript variant X3, mRNA.

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CloneID	OCo293160
Clone ID Related Accession (Same CDS sequence)	XM_021285854.1 , XM_021285857.1 , XM_021285856.1 , XM_021285855.1
Accession Version	XM_021285856.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-23
Organism	Columba livia(rock pigeon)
Product	pericentriolar material 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004973253.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Columba livia Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACAG GAGGTGGTCC CTTTGAAGAA GGCATGAATG ATCAAGACTT GCCTAGCTGG 
AGCAATGACA GCTTTGATGA CCGGCTGAAC AACACGGACT GGGGAAGTCA ACAGAAGAAA 
GCAAACAGAT CTTCAGAAAA AAACAAGAAA AAGCTTGGTG GGGAAGCTGA AACAAGGCTT 
ACTAATGATA TATCTCCAGA ATCCTCACCT GGAATAGGAC GACGAAAGAC CAGAACTCCT 
CATAGTTTTC CTCATGCTCG ATACATGACC CAGATGTCTG TTCCGGAGCA GGCTGAGCTA 
GAAAGGCTTA AACAAAGAAT AAACTTCAGT GATCTGGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGCA GGGCAACAGC TGCTAACAAC AAACGTCAAC TTAATGAAAA CAAAAAACCA 
TTCAACTTCC TGTCACTGCA GATAAACACT AACAAGAGCA AAGATCCTGC CTCATGTTCC 
CAAAAAAAGG AAAGTGGGGT GTCAGTCCAA CGTAAAGAGT TGTTTGGTGC TGCTCTGAGC 
AAGGATTTCT TGCAAAATTG TCAAGTCTCT GCTCAAGAAG ATGGAGGGGG AGAACCAGCT 
ATGGATAGTA GCCAGATTGT GAGCAGACTA GTTCAAATTC GCGACTATAT TGCTAAGGCC 
AGCTCCATGC GGGATGATCT TGTAGAGAAA AATGAAAGAT CGGCCAATGT TGAGCGTTTA 
TCACACCTTA TAGATCACCT TAAAGAGCAG GAGAAATCCT ATCTGAAATT TTTGCAAAAG 
ATGCTTGCTA GACAAAATGA GGAGGATGAT GTTCGGACTA TAGATTCAGC TGTGGGATCT 
GGTTCTGTAG GTGAGAGCAC ATCGCTAAAC ATTGATGTGC AGTCTGAGGC TTCAGATACC 
ACGGAGGTAT CTTTTAGTTT GAGCATTCGG CCCTGCATTG AGGACAAACT AGGGAATTCA 
GCTTCACAAG AACAGGTTAC AGACTTTGAT GTTACTGCAA GCCCTAAAGG GAAAAATGAC 
AGAGCTGCCC TGAATGACAG GGAAATCTGG CCTTGTAGGA TTAATAGCCG AGAACATGGA 
TTGCCTTCAA AGGCTAGAGA TCCTCAACAG GAGGCTAAAG AGGAGTTGGA GAACTTGAAG 
AAGCAGCATG ATTTATTGAA AAGGATGCTA AAACAGCAAG AGCAATTAAA GGCTCTTCAA 
GGAAGACAGG CAGCTCTTCT TGCTTTGCAG CATAAAGCAG AGCAAGCAAT TGCTGTCATG 
GATGATTCTG TTGTAACAGA GACTACAGGT AGTGTTTCAG GAGTAAGCCT TACATCAGAA 
CTGAATGAAG AATTGAATGA CTTAATTCAG CGCTTTCACA ACCAACTTCA TGATTCTCAG 
ACACAGTCTG TACCAGACAA TAGAAGGCAA GCAGAAAGTC TTTCACTTAC TAGAGAGGTT 
TCACAAAGCA GAAACTCTTC AATGTCTGAA CGCCTGTCAG ATGAGAAGGC GCAGCTTTTT 
AACAAGATGC GAATGTTGCA GGGTAAAAAG CAAAAAATGG ACAAACTGTT AGGAGAACTT 
CATACACTTC GTGACCAACA TCTAAATAAC TCCTCCTTTT TTCCTGCTTC AGGTTCTCCT 
CAAAGAAGTA TTGATCAAAG AAGTACAACT TCAGCTGCTT CTGGTCCTGC AGGCATAGTA 
ACTGTTGTCA ATGGCGAATC AAATAGCCTG GCATCTGCTC CCTATCCTCC TGATTCCCTG 
GTTTCTCAGA ATGAGAGTGA AGAAGATGAC AATCTAAATC CAACAGAAAA GCTTCAGAAA 
CTAAATGAAG TTCGTAAGAG GCTGAATGAG TTACGTGAGC TAGTTCACTA CTATGAGCAA 
ACATCTGATA TGATGACAGA TTCTGTGAAT GAAAACACTA AGGAAGAAGA AGAAACCGAA 
GAATCAGAAA GTGATTCTGA ACGTGAGGAT CCACAGCCTG TTACAAATAT TAGAAATCCT 
CAAGGAATCA GTAGTTGGAG TGACATAAAT AGCAACTCAA ATGTACAGTG TGGAACTAAT 
AACAGAGATG GAAGACATCT TAATACAGAC TGTGAAATAA ATAACAGATC TGCTGCTAAC 
ATAAGGACTC TAAAAATGTC GTCACCTTTA GACTGTCATA ATAGGGAGGA TGACAAAGAT 
ATTGGCCTAC CCCAATGTGA AGATGAAGAA GTGGAAGAAG ATAGAGTTAG TGAAGATTCT 
GCATCTAGTC ACAGAAGCAG CCTGGGTGAT ACTGCTGGAG ATGCTGAGTT TGAGCAGAAG 
ATCAATAGGC TTATAGCTGC AAAACAGAAA CTTAGACAAT TACAAAACCT TGCTGCTATG 
GTGCAGGATG ATGATCCAGC ACCTCAGACA ACAATTGCAA ATGCATCTAA TATTGGTGAC 
TTGTTTTTGG GTGAGATGGA AGAGACAAAG CAGCAGCCAA ACAATGTCCG AGCTAGTACC 
AACAAATTAC AAAAAGATGT AGAACTGAAT GAAAAAGCAA GAGAGAAGTT TTATGAAGCT 
AAACTCCAGC AGCAGCAACA GGAGCTTAAG CAGTTACAAG AAGAAAGAAG AAAACTGATT 
GAAATTCAAG AAAAAATTCA GGTGTTACAG AAAGCTTGTC CTGATCTTCA ATTGTCAGCT 
GGCTTGGGTG ACTGCACAGC AAATAGACAG ACTTCACCAG CAACATCAAC TCCAGCCATG 
AATGACACAG CTGGCAAGCC TTCACTTGAA TTTGATGAAT CTGTACCAGT AGGCAATGAG 
TTATGGTCTG AGATGAGAAG ACATGAGATT TTAAGAGAAG AATTGCGACA AAGAAGAAAG 
CAACTGGAAG CTCTAATGGC TGAGCATCAG AGGAGGAAAG AGCTCGCAGA AACAATATCT 
ACTGTTGCTG CGTCTGTTAA AAGTGAAGGA TCAGAAGCTC GGCGTACTCC ACAGCAGAGT 
AGGACTGAAA ATAGGACAAT GGCTACCTGG GGAGGTTCTA CGCAGTGTGC ACTAGATGAA 
GAGGATGGAG ATGAAGATGG TTATCTCTCT GATGGAGCTG GTCAGGCAGA AGAAGATGAT 
GATGATGCGT CAAGTGCGAA TGACAGCTTC TCTGTTTATC CCAATAACAA CGTACCAGAA 
AATGCATATT TTGTTAAAGA AAACAAGGAT AGGTGGAAAA ATTACCGTCC TCTTTCAGCA 
GATGGAAATT ATCGTCCATT GTCTAAGGCC AGACAACAGC AAAACATAAG TATGCGACGT 
CAGGAAAACC TTCGGTGGAT GTCTGAACTT TCATATGTGG AAGAAAAGGA ACGATGGCAA 
GAGCAGATCA ATCAGTTGAA GAAACAGCAT GAATTTAGTG TCAGCATTTG TCAAACTTTA 
ATGCAGGATC AGCAGACCCT CTCTTGTCTT CTACAGACTT TGCTTACAGG CCCTTACAGT 
ATGATGCCCA ATAATGTTGC ATCTTCACAA GTACATCTTA TTATGCATCA GCTAAACCAG 
TGTTACACTC AACTGACATG GCAGCAGAAT AACGTCCAGA GGTTGAAACA AATGTTAAAT 
GATCTTGTGC GTCAGCAAGA AGAACAACGG TATGAAGAGA AGTCATCAAG AAAGGATAGA 
GGCAGTGGTG TACCCCCACC TCCATCTCCT GTTTTCTGTC CGTTCAGCTT TCCTCCACAA 
CCTTTGAACC TGTTTAATGT ACCAGGATTT ACTAATTTTT CTTCCTTTGC TCCAGATATT 
AACTATAATC CAGTGTTTCC TTCTGGTTTT GGAGATTTTG CACACAATAT TTCTCCACAC 
AGCAGTGAGC AGCAGCAGCA ACATCCTCTA GATCATAATG CATCTGGGAA AACTGAGTAT 
ATGGCGTTCC CCAAACCTTT TGAAAGCAGT TCCTCTAATG GAGCGGAAAA ACAAAGGAGG 
AGTCACAGAC AACCTGAAGA GGAAATGGAA AAAAGATCAG CTTGGCTTAA TGATAGCCAG 
GACGTGAAAA AAGATGATCA GTCTCAGCTG AAAGCAAGTT TTGCTGTTTC AGTACAAAAC 
ATTGCTTCCA ATCATAAAAA TCAGTCTGAT ATGAGCAGAA GAAGAGAGTT TGATGAAGAG 
TCTTTGGAGA GTTTTAGTAG CATGCCTGAT CCAGTAGACC CCACTACTGT GACAAAGACA 
TTTAAATCTA GAAAAGCATC AGCACAAGCA AGTTTGGCAT CAAAAGATAA AACACCTAAA 
TCGAAGAATA AGAGGAAGAG TTCTTCTCAG CTAAAAGGCA GAATTACAAA TATTGATTAT 
GAAAGTGCCA GTGCTTCTAG TGTGTGTGAA CCCTGCAAGA GCAATAAAAG CAGACACTCT 
GAAGAGGTTG GTCATGCAAA GGTGTTCAGC AAAAGGAATC GGGAACAACT GGAAAAAATC 
ATTAAATACA GTAGATCTAC AGAAATGTCT TCAGCGCATG CTAGGAGAAT TCTGCAGCAG 
TCTAACAGAA ATGCATGCAT TGAAGCGCCA GAAACTGGTA GTGATCTTTC TATGTTTGAA 
GCTTTACGAG ACACAATTTA TTCTGAAGTG GCAACTCTTA TTTCTCAAAA TGAGTCTCGT 
CCCCACTTTC TTATTGAACT TTTCCATGAG CTTCAGCTGC TAAATACAGA TTACCTGAGG 
CAAAGAGCTC TATACGCTCT ACAGGATATA GTGACCAGAC ATTTATCTGA GAACAATGAA 
AAAGGGAAGT GCACAAAATC ACTGAATTCT GCAGCATGGA TAGCATCAAA TTCTGAACTC 
ACTCCCAGTG AAAGCCTTGC TTCTACAGAT GATGAAACTT TTGGCAAAAA CTTTTCTACA 
GAAGCATGTC AAGATTGTGA ACAAAATGAT GCAGACAATG GGAGTAGCAT GTCTACATCT 
TCAAATTTTG AACCCTTTGC CACTGATGAC CTTGGCAACA CAGTGATTCA CTTAGATCAA 
GCTTTGGCTA GGATGAGGGA GTATGAGCGT ATGAAAATTG AAGCTGAAAG TACCCTTGAC 
TCTGAGGGCT GCTCTAGTAA TTTTCAGGGT GCTTCTGCTG CTAAATTAGA AGGAGGTCAA 
AGTGCCAGTG AATGTGTTCC TGTGCCACAG TCAAGTGAAG CTTCTGCTGT TCCATGTCCT 
CATATAGACA CTCAGCAGCT TGACCGGCAG ATTAAAGCAA TTATGAAAGA GGTCATTCCT 
TTTCTGAAGG AACACATGGA TGAAGTATGC TCTTCGCAGT TACTGACATC AGTAAGACGT 
ATGGTCTTGA CCCTTACACA ACAAAATGAT GAAAGTAAAG AGTTTGTGAA GTTCTTTCAT 
AAGCAGCTTG GCAGTATACT TCAGGATTCA CTGGCAAAAT TTGCTGGTAG AAAACTGAAA 
GATTGTGGAG AGGATCTTCT AGTGGAGATC TCTGAAGTGT TATTTAATGA ATTAGCCTTT 
TTTAAACTCA TGCAAGACTT GGACAACAAC AGTATTTCTG TAAAGCAGAG ATGTAAAAGA 
AAAATAGAAG CTACTGAAGT GATACAGTCG TATGCTAAAG AGGCAAAAAA AGGTCTCCAG 
GTGGATGTTT GTTCATCTGC TGAAGATGTC GATGAGGACA AAGACAAGGA TGAGACTGAA 
ACTGTTAAAC AAGTACAGGA CTCAGAAATG TATGATGGGA ATAGAGTTCC TGAAAGTATT 
AGGTCTGATG CATCTGATCA GGAGGAAGAT GATGAAAGTG AAAGCGGTCC AGTGGCAATA 
AGTTTATCAA AAGCAGAAAC CCAAGCTCTG ACTAACTATG GTAGTGGAGA AGATGAGAAT 
GAAGATGAAG AAATAGAATT TGAGGAAGGA CCTGTTGATG TGCAGACGTC ATTACAAGCC 
AGCAGTGAAA CAACAACTGA AAATGAACAG AATTCAAACC AAGAACAAAG TAAGACAAAA 
AGCAGTGAGA TTCTGTCATC AGAACAAGAA TCTGTTTATG CTAAAGGTGA ACAACATGTG 
GCTACAATTT TGCCTTATTA CCTCAATGTA ATGGAGAATG CACCGCCTTT AACGGTCAAT 
ACCCCAGAAT CTTTTATAAC AGACAGTATG AAAACAGAAG AATCAAGTTC ATCTTTACCA 
GTAAATGAAA CTCAAACACT GGATACAACG TGTGTAGGAA ACAAATCTGC TGCAAGTTCT 
GAAAGCTCCA TGGCTGGCAG CCCTGATACA GAGTCACCTG TGTTAGTGAA TGAATATGAA 
GCTGGTTCTG GAAATGTAAG TCAAAGATCT GATGAAGATG ACTTTGTGAA AGTTGAAGAC 
TTGCCCCTTA AACTTGCAGT GTATTCAGAG GCAGATTTAA TGAAGAAAAT GGCAATGGAG 
GCCCAAACGA ACAGTTTGTC TGATGAATTA CTGGGTGGAG GTGGAGCTCA AGATCAAGAA 
TTAGTAGGAG ATGCCCAAAC TTTGAAAGAA CCTGAAACTT TTGGAGTTCA AAGTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021141531.1
CDS	102..6521
Translation	MATGGGPFEEGMNDQDLPSWSNDSFDDRLNNTDWGSQQKKANRSSEKNKKKLGGEAETRLTNDISPESSPGIGRRKTRTPHSFPHARYMTQMSVPEQAELERLKQRINFSDLDQRSIGSDSQGRATAANNKRQLNENKKPFNFLSLQINTNKSKDPASCSQKKESGVSVQRKELFGAALSKDFLQNCQVSAQEDGGGEPAMDSSQIVSRLVQIRDYIAKASSMRDDLVEKNERSANVERLSHLIDHLKEQEKSYLKFLQKMLARQNEEDDVRTIDSAVGSGSVGESTSLNIDVQSEASDTTEVSFSLSIRPCIEDKLGNSASQEQVTDFDVTASPKGKNDRAALNDREIWPCRINSREHGLPSKARDPQQEAKEELENLKKQHDLLKRMLKQQEQLKALQGRQAALLALQHKAEQAIAVMDDSVVTETTGSVSGVSLTSELNEELNDLIQRFHNQLHDSQTQSVPDNRRQAESLSLTREVSQSRNSSMSERLSDEKAQLFNKMRMLQGKKQKMDKLLGELHTLRDQHLNNSSFFPASGSPQRSIDQRSTTSAASGPAGIVTVVNGESNSLASAPYPPDSLVSQNESEEDDNLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDSVNENTKEEEETEESESDSEREDPQPVTNIRNPQGISSWSDINSNSNVQCGTNNRDGRHLNTDCEINNRSAANIRTLKMSSPLDCHNREDDKDIGLPQCEDEEVEEDRVSEDSASSHRSSLGDTAGDAEFEQKINRLIAAKQKLRQLQNLAAMVQDDDPAPQTTIANASNIGDLFLGEMEETKQQPNNVRASTNKLQKDVELNEKAREKFYEAKLQQQQQELKQLQEERRKLIEIQEKIQVLQKACPDLQLSAGLGDCTANRQTSPATSTPAMNDTAGKPSLEFDESVPVGNELWSEMRRHEILREELRQRRKQLEALMAEHQRRKELAETISTVAASVKSEGSEARRTPQQSRTENRTMATWGGSTQCALDEEDGDEDGYLSDGAGQAEEDDDDASSANDSFSVYPNNNVPENAYFVKENKDRWKNYRPLSADGNYRPLSKARQQQNISMRRQENLRWMSELSYVEEKERWQEQINQLKKQHEFSVSICQTLMQDQQTLSCLLQTLLTGPYSMMPNNVASSQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNDLVRQQEEQRYEEKSSRKDRGSGVPPPPSPVFCPFSFPPQPLNLFNVPGFTNFSSFAPDINYNPVFPSGFGDFAHNISPHSSEQQQQHPLDHNASGKTEYMAFPKPFESSSSNGAEKQRRSHRQPEEEMEKRSAWLNDSQDVKKDDQSQLKASFAVSVQNIASNHKNQSDMSRRREFDEESLESFSSMPDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKNKRKSSSQLKGRITNIDYESASASSVCEPCKSNKSRHSEEVGHAKVFSKRNREQLEKIIKYSRSTEMSSAHARRILQQSNRNACIEAPETGSDLSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHLSENNEKGKCTKSLNSAAWIASNSELTPSESLASTDDETFGKNFSTEACQDCEQNDADNGSSMSTSSNFEPFATDDLGNTVIHLDQALARMREYERMKIEAESTLDSEGCSSNFQGASAAKLEGGQSASECVPVPQSSEASAVPCPHIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSISVKQRCKRKIEATEVIQSYAKEAKKGLQVDVCSSAEDVDEDKDKDETETVKQVQDSEMYDGNRVPESIRSDASDQEEDDESESGPVAISLSKAETQALTNYGSGEDENEDEEIEFEEGPVDVQTSLQASSETTTENEQNSNQEQSKTKSSEILSSEQESVYAKGEQHVATILPYYLNVMENAPPLTVNTPESFITDSMKTEESSSSLPVNETQTLDTTCVGNKSAASSESSMAGSPDTESPVLVNEYEAGSGNVSQRSDEDDFVKVEDLPLKLAVYSEADLMKKMAMEAQTNSLSDELLGGGGAQDQELVGDAQTLKEPETFGVQSA

Target ORF information:

RefSeq Version	XM_021285856.1
Organism	Columba livia(rock pigeon)
Definition	Columba livia pericentriolar material 1 (PCM1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021285856.1

ORF Insert Sequence:

ATGGCAACAG GAGGTGGTCC CTTTGAAGAA GGCATGAATG ATCAAGACTT GCCTAGCTGG 
AGCAATGACA GCTTTGATGA CCGGCTGAAC AACACGGACT GGGGAAGTCA ACAGAAGAAA 
GCAAACAGAT CTTCAGAAAA AAACAAGAAA AAGCTTGGTG GGGAAGCTGA AACAAGGCTT 
ACTAATGATA TATCTCCAGA ATCCTCACCT GGAATAGGAC GACGAAAGAC CAGAACTCCT 
CATAGTTTTC CTCATGCTCG ATACATGACC CAGATGTCTG TTCCGGAGCA GGCTGAGCTA 
GAAAGGCTTA AACAAAGAAT AAACTTCAGT GATCTGGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGCA GGGCAACAGC TGCTAACAAC AAACGTCAAC TTAATGAAAA CAAAAAACCA 
TTCAACTTCC TGTCACTGCA GATAAACACT AACAAGAGCA AAGATCCTGC CTCATGTTCC 
CAAAAAAAGG AAAGTGGGGT GTCAGTCCAA CGTAAAGAGT TGTTTGGTGC TGCTCTGAGC 
AAGGATTTCT TGCAAAATTG TCAAGTCTCT GCTCAAGAAG ATGGAGGGGG AGAACCAGCT 
ATGGATAGTA GCCAGATTGT GAGCAGACTA GTTCAAATTC GCGACTATAT TGCTAAGGCC 
AGCTCCATGC GGGATGATCT TGTAGAGAAA AATGAAAGAT CGGCCAATGT TGAGCGTTTA 
TCACACCTTA TAGATCACCT TAAAGAGCAG GAGAAATCCT ATCTGAAATT TTTGCAAAAG 
ATGCTTGCTA GACAAAATGA GGAGGATGAT GTTCGGACTA TAGATTCAGC TGTGGGATCT 
GGTTCTGTAG GTGAGAGCAC ATCGCTAAAC ATTGATGTGC AGTCTGAGGC TTCAGATACC 
ACGGAGGTAT CTTTTAGTTT GAGCATTCGG CCCTGCATTG AGGACAAACT AGGGAATTCA 
GCTTCACAAG AACAGGTTAC AGACTTTGAT GTTACTGCAA GCCCTAAAGG GAAAAATGAC 
AGAGCTGCCC TGAATGACAG GGAAATCTGG CCTTGTAGGA TTAATAGCCG AGAACATGGA 
TTGCCTTCAA AGGCTAGAGA TCCTCAACAG GAGGCTAAAG AGGAGTTGGA GAACTTGAAG 
AAGCAGCATG ATTTATTGAA AAGGATGCTA AAACAGCAAG AGCAATTAAA GGCTCTTCAA 
GGAAGACAGG CAGCTCTTCT TGCTTTGCAG CATAAAGCAG AGCAAGCAAT TGCTGTCATG 
GATGATTCTG TTGTAACAGA GACTACAGGT AGTGTTTCAG GAGTAAGCCT TACATCAGAA 
CTGAATGAAG AATTGAATGA CTTAATTCAG CGCTTTCACA ACCAACTTCA TGATTCTCAG 
ACACAGTCTG TACCAGACAA TAGAAGGCAA GCAGAAAGTC TTTCACTTAC TAGAGAGGTT 
TCACAAAGCA GAAACTCTTC AATGTCTGAA CGCCTGTCAG ATGAGAAGGC GCAGCTTTTT 
AACAAGATGC GAATGTTGCA GGGTAAAAAG CAAAAAATGG ACAAACTGTT AGGAGAACTT 
CATACACTTC GTGACCAACA TCTAAATAAC TCCTCCTTTT TTCCTGCTTC AGGTTCTCCT 
CAAAGAAGTA TTGATCAAAG AAGTACAACT TCAGCTGCTT CTGGTCCTGC AGGCATAGTA 
ACTGTTGTCA ATGGCGAATC AAATAGCCTG GCATCTGCTC CCTATCCTCC TGATTCCCTG 
GTTTCTCAGA ATGAGAGTGA AGAAGATGAC AATCTAAATC CAACAGAAAA GCTTCAGAAA 
CTAAATGAAG TTCGTAAGAG GCTGAATGAG TTACGTGAGC TAGTTCACTA CTATGAGCAA 
ACATCTGATA TGATGACAGA TTCTGTGAAT GAAAACACTA AGGAAGAAGA AGAAACCGAA 
GAATCAGAAA GTGATTCTGA ACGTGAGGAT CCACAGCCTG TTACAAATAT TAGAAATCCT 
CAAGGAATCA GTAGTTGGAG TGACATAAAT AGCAACTCAA ATGTACAGTG TGGAACTAAT 
AACAGAGATG GAAGACATCT TAATACAGAC TGTGAAATAA ATAACAGATC TGCTGCTAAC 
ATAAGGACTC TAAAAATGTC GTCACCTTTA GACTGTCATA ATAGGGAGGA TGACAAAGAT 
ATTGGCCTAC CCCAATGTGA AGATGAAGAA GTGGAAGAAG ATAGAGTTAG TGAAGATTCT 
GCATCTAGTC ACAGAAGCAG CCTGGGTGAT ACTGCTGGAG ATGCTGAGTT TGAGCAGAAG 
ATCAATAGGC TTATAGCTGC AAAACAGAAA CTTAGACAAT TACAAAACCT TGCTGCTATG 
GTGCAGGATG ATGATCCAGC ACCTCAGACA ACAATTGCAA ATGCATCTAA TATTGGTGAC 
TTGTTTTTGG GTGAGATGGA AGAGACAAAG CAGCAGCCAA ACAATGTCCG AGCTAGTACC 
AACAAATTAC AAAAAGATGT AGAACTGAAT GAAAAAGCAA GAGAGAAGTT TTATGAAGCT 
AAACTCCAGC AGCAGCAACA GGAGCTTAAG CAGTTACAAG AAGAAAGAAG AAAACTGATT 
GAAATTCAAG AAAAAATTCA GGTGTTACAG AAAGCTTGTC CTGATCTTCA ATTGTCAGCT 
GGCTTGGGTG ACTGCACAGC AAATAGACAG ACTTCACCAG CAACATCAAC TCCAGCCATG 
AATGACACAG CTGGCAAGCC TTCACTTGAA TTTGATGAAT CTGTACCAGT AGGCAATGAG 
TTATGGTCTG AGATGAGAAG ACATGAGATT TTAAGAGAAG AATTGCGACA AAGAAGAAAG 
CAACTGGAAG CTCTAATGGC TGAGCATCAG AGGAGGAAAG AGCTCGCAGA AACAATATCT 
ACTGTTGCTG CGTCTGTTAA AAGTGAAGGA TCAGAAGCTC GGCGTACTCC ACAGCAGAGT 
AGGACTGAAA ATAGGACAAT GGCTACCTGG GGAGGTTCTA CGCAGTGTGC ACTAGATGAA 
GAGGATGGAG ATGAAGATGG TTATCTCTCT GATGGAGCTG GTCAGGCAGA AGAAGATGAT 
GATGATGCGT CAAGTGCGAA TGACAGCTTC TCTGTTTATC CCAATAACAA CGTACCAGAA 
AATGCATATT TTGTTAAAGA AAACAAGGAT AGGTGGAAAA ATTACCGTCC TCTTTCAGCA 
GATGGAAATT ATCGTCCATT GTCTAAGGCC AGACAACAGC AAAACATAAG TATGCGACGT 
CAGGAAAACC TTCGGTGGAT GTCTGAACTT TCATATGTGG AAGAAAAGGA ACGATGGCAA 
GAGCAGATCA ATCAGTTGAA GAAACAGCAT GAATTTAGTG TCAGCATTTG TCAAACTTTA 
ATGCAGGATC AGCAGACCCT CTCTTGTCTT CTACAGACTT TGCTTACAGG CCCTTACAGT 
ATGATGCCCA ATAATGTTGC ATCTTCACAA GTACATCTTA TTATGCATCA GCTAAACCAG 
TGTTACACTC AACTGACATG GCAGCAGAAT AACGTCCAGA GGTTGAAACA AATGTTAAAT 
GATCTTGTGC GTCAGCAAGA AGAACAACGG TATGAAGAGA AGTCATCAAG AAAGGATAGA 
GGCAGTGGTG TACCCCCACC TCCATCTCCT GTTTTCTGTC CGTTCAGCTT TCCTCCACAA 
CCTTTGAACC TGTTTAATGT ACCAGGATTT ACTAATTTTT CTTCCTTTGC TCCAGATATT 
AACTATAATC CAGTGTTTCC TTCTGGTTTT GGAGATTTTG CACACAATAT TTCTCCACAC 
AGCAGTGAGC AGCAGCAGCA ACATCCTCTA GATCATAATG CATCTGGGAA AACTGAGTAT 
ATGGCGTTCC CCAAACCTTT TGAAAGCAGT TCCTCTAATG GAGCGGAAAA ACAAAGGAGG 
AGTCACAGAC AACCTGAAGA GGAAATGGAA AAAAGATCAG CTTGGCTTAA TGATAGCCAG 
GACGTGAAAA AAGATGATCA GTCTCAGCTG AAAGCAAGTT TTGCTGTTTC AGTACAAAAC 
ATTGCTTCCA ATCATAAAAA TCAGTCTGAT ATGAGCAGAA GAAGAGAGTT TGATGAAGAG 
TCTTTGGAGA GTTTTAGTAG CATGCCTGAT CCAGTAGACC CCACTACTGT GACAAAGACA 
TTTAAATCTA GAAAAGCATC AGCACAAGCA AGTTTGGCAT CAAAAGATAA AACACCTAAA 
TCGAAGAATA AGAGGAAGAG TTCTTCTCAG CTAAAAGGCA GAATTACAAA TATTGATTAT 
GAAAGTGCCA GTGCTTCTAG TGTGTGTGAA CCCTGCAAGA GCAATAAAAG CAGACACTCT 
GAAGAGGTTG GTCATGCAAA GGTGTTCAGC AAAAGGAATC GGGAACAACT GGAAAAAATC 
ATTAAATACA GTAGATCTAC AGAAATGTCT TCAGCGCATG CTAGGAGAAT TCTGCAGCAG 
TCTAACAGAA ATGCATGCAT TGAAGCGCCA GAAACTGGTA GTGATCTTTC TATGTTTGAA 
GCTTTACGAG ACACAATTTA TTCTGAAGTG GCAACTCTTA TTTCTCAAAA TGAGTCTCGT 
CCCCACTTTC TTATTGAACT TTTCCATGAG CTTCAGCTGC TAAATACAGA TTACCTGAGG 
CAAAGAGCTC TATACGCTCT ACAGGATATA GTGACCAGAC ATTTATCTGA GAACAATGAA 
AAAGGGAAGT GCACAAAATC ACTGAATTCT GCAGCATGGA TAGCATCAAA TTCTGAACTC 
ACTCCCAGTG AAAGCCTTGC TTCTACAGAT GATGAAACTT TTGGCAAAAA CTTTTCTACA 
GAAGCATGTC AAGATTGTGA ACAAAATGAT GCAGACAATG GGAGTAGCAT GTCTACATCT 
TCAAATTTTG AACCCTTTGC CACTGATGAC CTTGGCAACA CAGTGATTCA CTTAGATCAA 
GCTTTGGCTA GGATGAGGGA GTATGAGCGT ATGAAAATTG AAGCTGAAAG TACCCTTGAC 
TCTGAGGGCT GCTCTAGTAA TTTTCAGGGT GCTTCTGCTG CTAAATTAGA AGGAGGTCAA 
AGTGCCAGTG AATGTGTTCC TGTGCCACAG TCAAGTGAAG CTTCTGCTGT TCCATGTCCT 
CATATAGACA CTCAGCAGCT TGACCGGCAG ATTAAAGCAA TTATGAAAGA GGTCATTCCT 
TTTCTGAAGG AACACATGGA TGAAGTATGC TCTTCGCAGT TACTGACATC AGTAAGACGT 
ATGGTCTTGA CCCTTACACA ACAAAATGAT GAAAGTAAAG AGTTTGTGAA GTTCTTTCAT 
AAGCAGCTTG GCAGTATACT TCAGGATTCA CTGGCAAAAT TTGCTGGTAG AAAACTGAAA 
GATTGTGGAG AGGATCTTCT AGTGGAGATC TCTGAAGTGT TATTTAATGA ATTAGCCTTT 
TTTAAACTCA TGCAAGACTT GGACAACAAC AGTATTTCTG TAAAGCAGAG ATGTAAAAGA 
AAAATAGAAG CTACTGAAGT GATACAGTCG TATGCTAAAG AGGCAAAAAA AGGTCTCCAG 
GTGGATGTTT GTTCATCTGC TGAAGATGTC GATGAGGACA AAGACAAGGA TGAGACTGAA 
ACTGTTAAAC AAGTACAGGA CTCAGAAATG TATGATGGGA ATAGAGTTCC TGAAAGTATT 
AGGTCTGATG CATCTGATCA GGAGGAAGAT GATGAAAGTG AAAGCGGTCC AGTGGCAATA 
AGTTTATCAA AAGCAGAAAC CCAAGCTCTG ACTAACTATG GTAGTGGAGA AGATGAGAAT 
GAAGATGAAG AAATAGAATT TGAGGAAGGA CCTGTTGATG TGCAGACGTC ATTACAAGCC 
AGCAGTGAAA CAACAACTGA AAATGAACAG AATTCAAACC AAGAACAAAG TAAGACAAAA 
AGCAGTGAGA TTCTGTCATC AGAACAAGAA TCTGTTTATG CTAAAGGTGA ACAACATGTG 
GCTACAATTT TGCCTTATTA CCTCAATGTA ATGGAGAATG CACCGCCTTT AACGGTCAAT 
ACCCCAGAAT CTTTTATAAC AGACAGTATG AAAACAGAAG AATCAAGTTC ATCTTTACCA 
GTAAATGAAA CTCAAACACT GGATACAACG TGTGTAGGAA ACAAATCTGC TGCAAGTTCT 
GAAAGCTCCA TGGCTGGCAG CCCTGATACA GAGTCACCTG TGTTAGTGAA TGAATATGAA 
GCTGGTTCTG GAAATGTAAG TCAAAGATCT GATGAAGATG ACTTTGTGAA AGTTGAAGAC 
TTGCCCCTTA AACTTGCAGT GTATTCAGAG GCAGATTTAA TGAAGAAAAT GGCAATGGAG 
GCCCAAACGA ACAGTTTGTC TGATGAATTA CTGGGTGGAG GTGGAGCTCA AGATCAAGAA 
TTAGTAGGAG ATGCCCAAAC TTTGAAAGAA CCTGAAACTT TTGGAGTTCA AAGTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_021285855.1 ) cDNA ORF clone, Columba livia(rock pigeon) -> XP_021141530.1 Columba livia pericentriolar material 1 (PCM1), transcript variant X2, mRNA.

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CloneID	OCo293160
Clone ID Related Accession (Same CDS sequence)	XM_021285854.1 , XM_021285857.1 , XM_021285856.1 , XM_021285855.1
Accession Version	XM_021285855.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-23
Organism	Columba livia(rock pigeon)
Product	pericentriolar material 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004973253.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Columba livia Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACAG GAGGTGGTCC CTTTGAAGAA GGCATGAATG ATCAAGACTT GCCTAGCTGG 
AGCAATGACA GCTTTGATGA CCGGCTGAAC AACACGGACT GGGGAAGTCA ACAGAAGAAA 
GCAAACAGAT CTTCAGAAAA AAACAAGAAA AAGCTTGGTG GGGAAGCTGA AACAAGGCTT 
ACTAATGATA TATCTCCAGA ATCCTCACCT GGAATAGGAC GACGAAAGAC CAGAACTCCT 
CATAGTTTTC CTCATGCTCG ATACATGACC CAGATGTCTG TTCCGGAGCA GGCTGAGCTA 
GAAAGGCTTA AACAAAGAAT AAACTTCAGT GATCTGGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGCA GGGCAACAGC TGCTAACAAC AAACGTCAAC TTAATGAAAA CAAAAAACCA 
TTCAACTTCC TGTCACTGCA GATAAACACT AACAAGAGCA AAGATCCTGC CTCATGTTCC 
CAAAAAAAGG AAAGTGGGGT GTCAGTCCAA CGTAAAGAGT TGTTTGGTGC TGCTCTGAGC 
AAGGATTTCT TGCAAAATTG TCAAGTCTCT GCTCAAGAAG ATGGAGGGGG AGAACCAGCT 
ATGGATAGTA GCCAGATTGT GAGCAGACTA GTTCAAATTC GCGACTATAT TGCTAAGGCC 
AGCTCCATGC GGGATGATCT TGTAGAGAAA AATGAAAGAT CGGCCAATGT TGAGCGTTTA 
TCACACCTTA TAGATCACCT TAAAGAGCAG GAGAAATCCT ATCTGAAATT TTTGCAAAAG 
ATGCTTGCTA GACAAAATGA GGAGGATGAT GTTCGGACTA TAGATTCAGC TGTGGGATCT 
GGTTCTGTAG GTGAGAGCAC ATCGCTAAAC ATTGATGTGC AGTCTGAGGC TTCAGATACC 
ACGGAGGTAT CTTTTAGTTT GAGCATTCGG CCCTGCATTG AGGACAAACT AGGGAATTCA 
GCTTCACAAG AACAGGTTAC AGACTTTGAT GTTACTGCAA GCCCTAAAGG GAAAAATGAC 
AGAGCTGCCC TGAATGACAG GGAAATCTGG CCTTGTAGGA TTAATAGCCG AGAACATGGA 
TTGCCTTCAA AGGCTAGAGA TCCTCAACAG GAGGCTAAAG AGGAGTTGGA GAACTTGAAG 
AAGCAGCATG ATTTATTGAA AAGGATGCTA AAACAGCAAG AGCAATTAAA GGCTCTTCAA 
GGAAGACAGG CAGCTCTTCT TGCTTTGCAG CATAAAGCAG AGCAAGCAAT TGCTGTCATG 
GATGATTCTG TTGTAACAGA GACTACAGGT AGTGTTTCAG GAGTAAGCCT TACATCAGAA 
CTGAATGAAG AATTGAATGA CTTAATTCAG CGCTTTCACA ACCAACTTCA TGATTCTCAG 
ACACAGTCTG TACCAGACAA TAGAAGGCAA GCAGAAAGTC TTTCACTTAC TAGAGAGGTT 
TCACAAAGCA GAAACTCTTC AATGTCTGAA CGCCTGTCAG ATGAGAAGGC GCAGCTTTTT 
AACAAGATGC GAATGTTGCA GGGTAAAAAG CAAAAAATGG ACAAACTGTT AGGAGAACTT 
CATACACTTC GTGACCAACA TCTAAATAAC TCCTCCTTTT TTCCTGCTTC AGGTTCTCCT 
CAAAGAAGTA TTGATCAAAG AAGTACAACT TCAGCTGCTT CTGGTCCTGC AGGCATAGTA 
ACTGTTGTCA ATGGCGAATC AAATAGCCTG GCATCTGCTC CCTATCCTCC TGATTCCCTG 
GTTTCTCAGA ATGAGAGTGA AGAAGATGAC AATCTAAATC CAACAGAAAA GCTTCAGAAA 
CTAAATGAAG TTCGTAAGAG GCTGAATGAG TTACGTGAGC TAGTTCACTA CTATGAGCAA 
ACATCTGATA TGATGACAGA TTCTGTGAAT GAAAACACTA AGGAAGAAGA AGAAACCGAA 
GAATCAGAAA GTGATTCTGA ACGTGAGGAT CCACAGCCTG TTACAAATAT TAGAAATCCT 
CAAGGAATCA GTAGTTGGAG TGACATAAAT AGCAACTCAA ATGTACAGTG TGGAACTAAT 
AACAGAGATG GAAGACATCT TAATACAGAC TGTGAAATAA ATAACAGATC TGCTGCTAAC 
ATAAGGACTC TAAAAATGTC GTCACCTTTA GACTGTCATA ATAGGGAGGA TGACAAAGAT 
ATTGGCCTAC CCCAATGTGA AGATGAAGAA GTGGAAGAAG ATAGAGTTAG TGAAGATTCT 
GCATCTAGTC ACAGAAGCAG CCTGGGTGAT ACTGCTGGAG ATGCTGAGTT TGAGCAGAAG 
ATCAATAGGC TTATAGCTGC AAAACAGAAA CTTAGACAAT TACAAAACCT TGCTGCTATG 
GTGCAGGATG ATGATCCAGC ACCTCAGACA ACAATTGCAA ATGCATCTAA TATTGGTGAC 
TTGTTTTTGG GTGAGATGGA AGAGACAAAG CAGCAGCCAA ACAATGTCCG AGCTAGTACC 
AACAAATTAC AAAAAGATGT AGAACTGAAT GAAAAAGCAA GAGAGAAGTT TTATGAAGCT 
AAACTCCAGC AGCAGCAACA GGAGCTTAAG CAGTTACAAG AAGAAAGAAG AAAACTGATT 
GAAATTCAAG AAAAAATTCA GGTGTTACAG AAAGCTTGTC CTGATCTTCA ATTGTCAGCT 
GGCTTGGGTG ACTGCACAGC AAATAGACAG ACTTCACCAG CAACATCAAC TCCAGCCATG 
AATGACACAG CTGGCAAGCC TTCACTTGAA TTTGATGAAT CTGTACCAGT AGGCAATGAG 
TTATGGTCTG AGATGAGAAG ACATGAGATT TTAAGAGAAG AATTGCGACA AAGAAGAAAG 
CAACTGGAAG CTCTAATGGC TGAGCATCAG AGGAGGAAAG AGCTCGCAGA AACAATATCT 
ACTGTTGCTG CGTCTGTTAA AAGTGAAGGA TCAGAAGCTC GGCGTACTCC ACAGCAGAGT 
AGGACTGAAA ATAGGACAAT GGCTACCTGG GGAGGTTCTA CGCAGTGTGC ACTAGATGAA 
GAGGATGGAG ATGAAGATGG TTATCTCTCT GATGGAGCTG GTCAGGCAGA AGAAGATGAT 
GATGATGCGT CAAGTGCGAA TGACAGCTTC TCTGTTTATC CCAATAACAA CGTACCAGAA 
AATGCATATT TTGTTAAAGA AAACAAGGAT AGGTGGAAAA ATTACCGTCC TCTTTCAGCA 
GATGGAAATT ATCGTCCATT GTCTAAGGCC AGACAACAGC AAAACATAAG TATGCGACGT 
CAGGAAAACC TTCGGTGGAT GTCTGAACTT TCATATGTGG AAGAAAAGGA ACGATGGCAA 
GAGCAGATCA ATCAGTTGAA GAAACAGCAT GAATTTAGTG TCAGCATTTG TCAAACTTTA 
ATGCAGGATC AGCAGACCCT CTCTTGTCTT CTACAGACTT TGCTTACAGG CCCTTACAGT 
ATGATGCCCA ATAATGTTGC ATCTTCACAA GTACATCTTA TTATGCATCA GCTAAACCAG 
TGTTACACTC AACTGACATG GCAGCAGAAT AACGTCCAGA GGTTGAAACA AATGTTAAAT 
GATCTTGTGC GTCAGCAAGA AGAACAACGG TATGAAGAGA AGTCATCAAG AAAGGATAGA 
GGCAGTGGTG TACCCCCACC TCCATCTCCT GTTTTCTGTC CGTTCAGCTT TCCTCCACAA 
CCTTTGAACC TGTTTAATGT ACCAGGATTT ACTAATTTTT CTTCCTTTGC TCCAGATATT 
AACTATAATC CAGTGTTTCC TTCTGGTTTT GGAGATTTTG CACACAATAT TTCTCCACAC 
AGCAGTGAGC AGCAGCAGCA ACATCCTCTA GATCATAATG CATCTGGGAA AACTGAGTAT 
ATGGCGTTCC CCAAACCTTT TGAAAGCAGT TCCTCTAATG GAGCGGAAAA ACAAAGGAGG 
AGTCACAGAC AACCTGAAGA GGAAATGGAA AAAAGATCAG CTTGGCTTAA TGATAGCCAG 
GACGTGAAAA AAGATGATCA GTCTCAGCTG AAAGCAAGTT TTGCTGTTTC AGTACAAAAC 
ATTGCTTCCA ATCATAAAAA TCAGTCTGAT ATGAGCAGAA GAAGAGAGTT TGATGAAGAG 
TCTTTGGAGA GTTTTAGTAG CATGCCTGAT CCAGTAGACC CCACTACTGT GACAAAGACA 
TTTAAATCTA GAAAAGCATC AGCACAAGCA AGTTTGGCAT CAAAAGATAA AACACCTAAA 
TCGAAGAATA AGAGGAAGAG TTCTTCTCAG CTAAAAGGCA GAATTACAAA TATTGATTAT 
GAAAGTGCCA GTGCTTCTAG TGTGTGTGAA CCCTGCAAGA GCAATAAAAG CAGACACTCT 
GAAGAGGTTG GTCATGCAAA GGTGTTCAGC AAAAGGAATC GGGAACAACT GGAAAAAATC 
ATTAAATACA GTAGATCTAC AGAAATGTCT TCAGCGCATG CTAGGAGAAT TCTGCAGCAG 
TCTAACAGAA ATGCATGCAT TGAAGCGCCA GAAACTGGTA GTGATCTTTC TATGTTTGAA 
GCTTTACGAG ACACAATTTA TTCTGAAGTG GCAACTCTTA TTTCTCAAAA TGAGTCTCGT 
CCCCACTTTC TTATTGAACT TTTCCATGAG CTTCAGCTGC TAAATACAGA TTACCTGAGG 
CAAAGAGCTC TATACGCTCT ACAGGATATA GTGACCAGAC ATTTATCTGA GAACAATGAA 
AAAGGGAAGT GCACAAAATC ACTGAATTCT GCAGCATGGA TAGCATCAAA TTCTGAACTC 
ACTCCCAGTG AAAGCCTTGC TTCTACAGAT GATGAAACTT TTGGCAAAAA CTTTTCTACA 
GAAGCATGTC AAGATTGTGA ACAAAATGAT GCAGACAATG GGAGTAGCAT GTCTACATCT 
TCAAATTTTG AACCCTTTGC CACTGATGAC CTTGGCAACA CAGTGATTCA CTTAGATCAA 
GCTTTGGCTA GGATGAGGGA GTATGAGCGT ATGAAAATTG AAGCTGAAAG TACCCTTGAC 
TCTGAGGGCT GCTCTAGTAA TTTTCAGGGT GCTTCTGCTG CTAAATTAGA AGGAGGTCAA 
AGTGCCAGTG AATGTGTTCC TGTGCCACAG TCAAGTGAAG CTTCTGCTGT TCCATGTCCT 
CATATAGACA CTCAGCAGCT TGACCGGCAG ATTAAAGCAA TTATGAAAGA GGTCATTCCT 
TTTCTGAAGG AACACATGGA TGAAGTATGC TCTTCGCAGT TACTGACATC AGTAAGACGT 
ATGGTCTTGA CCCTTACACA ACAAAATGAT GAAAGTAAAG AGTTTGTGAA GTTCTTTCAT 
AAGCAGCTTG GCAGTATACT TCAGGATTCA CTGGCAAAAT TTGCTGGTAG AAAACTGAAA 
GATTGTGGAG AGGATCTTCT AGTGGAGATC TCTGAAGTGT TATTTAATGA ATTAGCCTTT 
TTTAAACTCA TGCAAGACTT GGACAACAAC AGTATTTCTG TAAAGCAGAG ATGTAAAAGA 
AAAATAGAAG CTACTGAAGT GATACAGTCG TATGCTAAAG AGGCAAAAAA AGGTCTCCAG 
GTGGATGTTT GTTCATCTGC TGAAGATGTC GATGAGGACA AAGACAAGGA TGAGACTGAA 
ACTGTTAAAC AAGTACAGGA CTCAGAAATG TATGATGGGA ATAGAGTTCC TGAAAGTATT 
AGGTCTGATG CATCTGATCA GGAGGAAGAT GATGAAAGTG AAAGCGGTCC AGTGGCAATA 
AGTTTATCAA AAGCAGAAAC CCAAGCTCTG ACTAACTATG GTAGTGGAGA AGATGAGAAT 
GAAGATGAAG AAATAGAATT TGAGGAAGGA CCTGTTGATG TGCAGACGTC ATTACAAGCC 
AGCAGTGAAA CAACAACTGA AAATGAACAG AATTCAAACC AAGAACAAAG TAAGACAAAA 
AGCAGTGAGA TTCTGTCATC AGAACAAGAA TCTGTTTATG CTAAAGGTGA ACAACATGTG 
GCTACAATTT TGCCTTATTA CCTCAATGTA ATGGAGAATG CACCGCCTTT AACGGTCAAT 
ACCCCAGAAT CTTTTATAAC AGACAGTATG AAAACAGAAG AATCAAGTTC ATCTTTACCA 
GTAAATGAAA CTCAAACACT GGATACAACG TGTGTAGGAA ACAAATCTGC TGCAAGTTCT 
GAAAGCTCCA TGGCTGGCAG CCCTGATACA GAGTCACCTG TGTTAGTGAA TGAATATGAA 
GCTGGTTCTG GAAATGTAAG TCAAAGATCT GATGAAGATG ACTTTGTGAA AGTTGAAGAC 
TTGCCCCTTA AACTTGCAGT GTATTCAGAG GCAGATTTAA TGAAGAAAAT GGCAATGGAG 
GCCCAAACGA ACAGTTTGTC TGATGAATTA CTGGGTGGAG GTGGAGCTCA AGATCAAGAA 
TTAGTAGGAG ATGCCCAAAC TTTGAAAGAA CCTGAAACTT TTGGAGTTCA AAGTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021141530.1
CDS	132..6551
Translation	MATGGGPFEEGMNDQDLPSWSNDSFDDRLNNTDWGSQQKKANRSSEKNKKKLGGEAETRLTNDISPESSPGIGRRKTRTPHSFPHARYMTQMSVPEQAELERLKQRINFSDLDQRSIGSDSQGRATAANNKRQLNENKKPFNFLSLQINTNKSKDPASCSQKKESGVSVQRKELFGAALSKDFLQNCQVSAQEDGGGEPAMDSSQIVSRLVQIRDYIAKASSMRDDLVEKNERSANVERLSHLIDHLKEQEKSYLKFLQKMLARQNEEDDVRTIDSAVGSGSVGESTSLNIDVQSEASDTTEVSFSLSIRPCIEDKLGNSASQEQVTDFDVTASPKGKNDRAALNDREIWPCRINSREHGLPSKARDPQQEAKEELENLKKQHDLLKRMLKQQEQLKALQGRQAALLALQHKAEQAIAVMDDSVVTETTGSVSGVSLTSELNEELNDLIQRFHNQLHDSQTQSVPDNRRQAESLSLTREVSQSRNSSMSERLSDEKAQLFNKMRMLQGKKQKMDKLLGELHTLRDQHLNNSSFFPASGSPQRSIDQRSTTSAASGPAGIVTVVNGESNSLASAPYPPDSLVSQNESEEDDNLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDSVNENTKEEEETEESESDSEREDPQPVTNIRNPQGISSWSDINSNSNVQCGTNNRDGRHLNTDCEINNRSAANIRTLKMSSPLDCHNREDDKDIGLPQCEDEEVEEDRVSEDSASSHRSSLGDTAGDAEFEQKINRLIAAKQKLRQLQNLAAMVQDDDPAPQTTIANASNIGDLFLGEMEETKQQPNNVRASTNKLQKDVELNEKAREKFYEAKLQQQQQELKQLQEERRKLIEIQEKIQVLQKACPDLQLSAGLGDCTANRQTSPATSTPAMNDTAGKPSLEFDESVPVGNELWSEMRRHEILREELRQRRKQLEALMAEHQRRKELAETISTVAASVKSEGSEARRTPQQSRTENRTMATWGGSTQCALDEEDGDEDGYLSDGAGQAEEDDDDASSANDSFSVYPNNNVPENAYFVKENKDRWKNYRPLSADGNYRPLSKARQQQNISMRRQENLRWMSELSYVEEKERWQEQINQLKKQHEFSVSICQTLMQDQQTLSCLLQTLLTGPYSMMPNNVASSQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNDLVRQQEEQRYEEKSSRKDRGSGVPPPPSPVFCPFSFPPQPLNLFNVPGFTNFSSFAPDINYNPVFPSGFGDFAHNISPHSSEQQQQHPLDHNASGKTEYMAFPKPFESSSSNGAEKQRRSHRQPEEEMEKRSAWLNDSQDVKKDDQSQLKASFAVSVQNIASNHKNQSDMSRRREFDEESLESFSSMPDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKNKRKSSSQLKGRITNIDYESASASSVCEPCKSNKSRHSEEVGHAKVFSKRNREQLEKIIKYSRSTEMSSAHARRILQQSNRNACIEAPETGSDLSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHLSENNEKGKCTKSLNSAAWIASNSELTPSESLASTDDETFGKNFSTEACQDCEQNDADNGSSMSTSSNFEPFATDDLGNTVIHLDQALARMREYERMKIEAESTLDSEGCSSNFQGASAAKLEGGQSASECVPVPQSSEASAVPCPHIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSISVKQRCKRKIEATEVIQSYAKEAKKGLQVDVCSSAEDVDEDKDKDETETVKQVQDSEMYDGNRVPESIRSDASDQEEDDESESGPVAISLSKAETQALTNYGSGEDENEDEEIEFEEGPVDVQTSLQASSETTTENEQNSNQEQSKTKSSEILSSEQESVYAKGEQHVATILPYYLNVMENAPPLTVNTPESFITDSMKTEESSSSLPVNETQTLDTTCVGNKSAASSESSMAGSPDTESPVLVNEYEAGSGNVSQRSDEDDFVKVEDLPLKLAVYSEADLMKKMAMEAQTNSLSDELLGGGGAQDQELVGDAQTLKEPETFGVQSA

Target ORF information:

RefSeq Version	XM_021285855.1
Organism	Columba livia(rock pigeon)
Definition	Columba livia pericentriolar material 1 (PCM1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021285855.1

ORF Insert Sequence:

ATGGCAACAG GAGGTGGTCC CTTTGAAGAA GGCATGAATG ATCAAGACTT GCCTAGCTGG 
AGCAATGACA GCTTTGATGA CCGGCTGAAC AACACGGACT GGGGAAGTCA ACAGAAGAAA 
GCAAACAGAT CTTCAGAAAA AAACAAGAAA AAGCTTGGTG GGGAAGCTGA AACAAGGCTT 
ACTAATGATA TATCTCCAGA ATCCTCACCT GGAATAGGAC GACGAAAGAC CAGAACTCCT 
CATAGTTTTC CTCATGCTCG ATACATGACC CAGATGTCTG TTCCGGAGCA GGCTGAGCTA 
GAAAGGCTTA AACAAAGAAT AAACTTCAGT GATCTGGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGCA GGGCAACAGC TGCTAACAAC AAACGTCAAC TTAATGAAAA CAAAAAACCA 
TTCAACTTCC TGTCACTGCA GATAAACACT AACAAGAGCA AAGATCCTGC CTCATGTTCC 
CAAAAAAAGG AAAGTGGGGT GTCAGTCCAA CGTAAAGAGT TGTTTGGTGC TGCTCTGAGC 
AAGGATTTCT TGCAAAATTG TCAAGTCTCT GCTCAAGAAG ATGGAGGGGG AGAACCAGCT 
ATGGATAGTA GCCAGATTGT GAGCAGACTA GTTCAAATTC GCGACTATAT TGCTAAGGCC 
AGCTCCATGC GGGATGATCT TGTAGAGAAA AATGAAAGAT CGGCCAATGT TGAGCGTTTA 
TCACACCTTA TAGATCACCT TAAAGAGCAG GAGAAATCCT ATCTGAAATT TTTGCAAAAG 
ATGCTTGCTA GACAAAATGA GGAGGATGAT GTTCGGACTA TAGATTCAGC TGTGGGATCT 
GGTTCTGTAG GTGAGAGCAC ATCGCTAAAC ATTGATGTGC AGTCTGAGGC TTCAGATACC 
ACGGAGGTAT CTTTTAGTTT GAGCATTCGG CCCTGCATTG AGGACAAACT AGGGAATTCA 
GCTTCACAAG AACAGGTTAC AGACTTTGAT GTTACTGCAA GCCCTAAAGG GAAAAATGAC 
AGAGCTGCCC TGAATGACAG GGAAATCTGG CCTTGTAGGA TTAATAGCCG AGAACATGGA 
TTGCCTTCAA AGGCTAGAGA TCCTCAACAG GAGGCTAAAG AGGAGTTGGA GAACTTGAAG 
AAGCAGCATG ATTTATTGAA AAGGATGCTA AAACAGCAAG AGCAATTAAA GGCTCTTCAA 
GGAAGACAGG CAGCTCTTCT TGCTTTGCAG CATAAAGCAG AGCAAGCAAT TGCTGTCATG 
GATGATTCTG TTGTAACAGA GACTACAGGT AGTGTTTCAG GAGTAAGCCT TACATCAGAA 
CTGAATGAAG AATTGAATGA CTTAATTCAG CGCTTTCACA ACCAACTTCA TGATTCTCAG 
ACACAGTCTG TACCAGACAA TAGAAGGCAA GCAGAAAGTC TTTCACTTAC TAGAGAGGTT 
TCACAAAGCA GAAACTCTTC AATGTCTGAA CGCCTGTCAG ATGAGAAGGC GCAGCTTTTT 
AACAAGATGC GAATGTTGCA GGGTAAAAAG CAAAAAATGG ACAAACTGTT AGGAGAACTT 
CATACACTTC GTGACCAACA TCTAAATAAC TCCTCCTTTT TTCCTGCTTC AGGTTCTCCT 
CAAAGAAGTA TTGATCAAAG AAGTACAACT TCAGCTGCTT CTGGTCCTGC AGGCATAGTA 
ACTGTTGTCA ATGGCGAATC AAATAGCCTG GCATCTGCTC CCTATCCTCC TGATTCCCTG 
GTTTCTCAGA ATGAGAGTGA AGAAGATGAC AATCTAAATC CAACAGAAAA GCTTCAGAAA 
CTAAATGAAG TTCGTAAGAG GCTGAATGAG TTACGTGAGC TAGTTCACTA CTATGAGCAA 
ACATCTGATA TGATGACAGA TTCTGTGAAT GAAAACACTA AGGAAGAAGA AGAAACCGAA 
GAATCAGAAA GTGATTCTGA ACGTGAGGAT CCACAGCCTG TTACAAATAT TAGAAATCCT 
CAAGGAATCA GTAGTTGGAG TGACATAAAT AGCAACTCAA ATGTACAGTG TGGAACTAAT 
AACAGAGATG GAAGACATCT TAATACAGAC TGTGAAATAA ATAACAGATC TGCTGCTAAC 
ATAAGGACTC TAAAAATGTC GTCACCTTTA GACTGTCATA ATAGGGAGGA TGACAAAGAT 
ATTGGCCTAC CCCAATGTGA AGATGAAGAA GTGGAAGAAG ATAGAGTTAG TGAAGATTCT 
GCATCTAGTC ACAGAAGCAG CCTGGGTGAT ACTGCTGGAG ATGCTGAGTT TGAGCAGAAG 
ATCAATAGGC TTATAGCTGC AAAACAGAAA CTTAGACAAT TACAAAACCT TGCTGCTATG 
GTGCAGGATG ATGATCCAGC ACCTCAGACA ACAATTGCAA ATGCATCTAA TATTGGTGAC 
TTGTTTTTGG GTGAGATGGA AGAGACAAAG CAGCAGCCAA ACAATGTCCG AGCTAGTACC 
AACAAATTAC AAAAAGATGT AGAACTGAAT GAAAAAGCAA GAGAGAAGTT TTATGAAGCT 
AAACTCCAGC AGCAGCAACA GGAGCTTAAG CAGTTACAAG AAGAAAGAAG AAAACTGATT 
GAAATTCAAG AAAAAATTCA GGTGTTACAG AAAGCTTGTC CTGATCTTCA ATTGTCAGCT 
GGCTTGGGTG ACTGCACAGC AAATAGACAG ACTTCACCAG CAACATCAAC TCCAGCCATG 
AATGACACAG CTGGCAAGCC TTCACTTGAA TTTGATGAAT CTGTACCAGT AGGCAATGAG 
TTATGGTCTG AGATGAGAAG ACATGAGATT TTAAGAGAAG AATTGCGACA AAGAAGAAAG 
CAACTGGAAG CTCTAATGGC TGAGCATCAG AGGAGGAAAG AGCTCGCAGA AACAATATCT 
ACTGTTGCTG CGTCTGTTAA AAGTGAAGGA TCAGAAGCTC GGCGTACTCC ACAGCAGAGT 
AGGACTGAAA ATAGGACAAT GGCTACCTGG GGAGGTTCTA CGCAGTGTGC ACTAGATGAA 
GAGGATGGAG ATGAAGATGG TTATCTCTCT GATGGAGCTG GTCAGGCAGA AGAAGATGAT 
GATGATGCGT CAAGTGCGAA TGACAGCTTC TCTGTTTATC CCAATAACAA CGTACCAGAA 
AATGCATATT TTGTTAAAGA AAACAAGGAT AGGTGGAAAA ATTACCGTCC TCTTTCAGCA 
GATGGAAATT ATCGTCCATT GTCTAAGGCC AGACAACAGC AAAACATAAG TATGCGACGT 
CAGGAAAACC TTCGGTGGAT GTCTGAACTT TCATATGTGG AAGAAAAGGA ACGATGGCAA 
GAGCAGATCA ATCAGTTGAA GAAACAGCAT GAATTTAGTG TCAGCATTTG TCAAACTTTA 
ATGCAGGATC AGCAGACCCT CTCTTGTCTT CTACAGACTT TGCTTACAGG CCCTTACAGT 
ATGATGCCCA ATAATGTTGC ATCTTCACAA GTACATCTTA TTATGCATCA GCTAAACCAG 
TGTTACACTC AACTGACATG GCAGCAGAAT AACGTCCAGA GGTTGAAACA AATGTTAAAT 
GATCTTGTGC GTCAGCAAGA AGAACAACGG TATGAAGAGA AGTCATCAAG AAAGGATAGA 
GGCAGTGGTG TACCCCCACC TCCATCTCCT GTTTTCTGTC CGTTCAGCTT TCCTCCACAA 
CCTTTGAACC TGTTTAATGT ACCAGGATTT ACTAATTTTT CTTCCTTTGC TCCAGATATT 
AACTATAATC CAGTGTTTCC TTCTGGTTTT GGAGATTTTG CACACAATAT TTCTCCACAC 
AGCAGTGAGC AGCAGCAGCA ACATCCTCTA GATCATAATG CATCTGGGAA AACTGAGTAT 
ATGGCGTTCC CCAAACCTTT TGAAAGCAGT TCCTCTAATG GAGCGGAAAA ACAAAGGAGG 
AGTCACAGAC AACCTGAAGA GGAAATGGAA AAAAGATCAG CTTGGCTTAA TGATAGCCAG 
GACGTGAAAA AAGATGATCA GTCTCAGCTG AAAGCAAGTT TTGCTGTTTC AGTACAAAAC 
ATTGCTTCCA ATCATAAAAA TCAGTCTGAT ATGAGCAGAA GAAGAGAGTT TGATGAAGAG 
TCTTTGGAGA GTTTTAGTAG CATGCCTGAT CCAGTAGACC CCACTACTGT GACAAAGACA 
TTTAAATCTA GAAAAGCATC AGCACAAGCA AGTTTGGCAT CAAAAGATAA AACACCTAAA 
TCGAAGAATA AGAGGAAGAG TTCTTCTCAG CTAAAAGGCA GAATTACAAA TATTGATTAT 
GAAAGTGCCA GTGCTTCTAG TGTGTGTGAA CCCTGCAAGA GCAATAAAAG CAGACACTCT 
GAAGAGGTTG GTCATGCAAA GGTGTTCAGC AAAAGGAATC GGGAACAACT GGAAAAAATC 
ATTAAATACA GTAGATCTAC AGAAATGTCT TCAGCGCATG CTAGGAGAAT TCTGCAGCAG 
TCTAACAGAA ATGCATGCAT TGAAGCGCCA GAAACTGGTA GTGATCTTTC TATGTTTGAA 
GCTTTACGAG ACACAATTTA TTCTGAAGTG GCAACTCTTA TTTCTCAAAA TGAGTCTCGT 
CCCCACTTTC TTATTGAACT TTTCCATGAG CTTCAGCTGC TAAATACAGA TTACCTGAGG 
CAAAGAGCTC TATACGCTCT ACAGGATATA GTGACCAGAC ATTTATCTGA GAACAATGAA 
AAAGGGAAGT GCACAAAATC ACTGAATTCT GCAGCATGGA TAGCATCAAA TTCTGAACTC 
ACTCCCAGTG AAAGCCTTGC TTCTACAGAT GATGAAACTT TTGGCAAAAA CTTTTCTACA 
GAAGCATGTC AAGATTGTGA ACAAAATGAT GCAGACAATG GGAGTAGCAT GTCTACATCT 
TCAAATTTTG AACCCTTTGC CACTGATGAC CTTGGCAACA CAGTGATTCA CTTAGATCAA 
GCTTTGGCTA GGATGAGGGA GTATGAGCGT ATGAAAATTG AAGCTGAAAG TACCCTTGAC 
TCTGAGGGCT GCTCTAGTAA TTTTCAGGGT GCTTCTGCTG CTAAATTAGA AGGAGGTCAA 
AGTGCCAGTG AATGTGTTCC TGTGCCACAG TCAAGTGAAG CTTCTGCTGT TCCATGTCCT 
CATATAGACA CTCAGCAGCT TGACCGGCAG ATTAAAGCAA TTATGAAAGA GGTCATTCCT 
TTTCTGAAGG AACACATGGA TGAAGTATGC TCTTCGCAGT TACTGACATC AGTAAGACGT 
ATGGTCTTGA CCCTTACACA ACAAAATGAT GAAAGTAAAG AGTTTGTGAA GTTCTTTCAT 
AAGCAGCTTG GCAGTATACT TCAGGATTCA CTGGCAAAAT TTGCTGGTAG AAAACTGAAA 
GATTGTGGAG AGGATCTTCT AGTGGAGATC TCTGAAGTGT TATTTAATGA ATTAGCCTTT 
TTTAAACTCA TGCAAGACTT GGACAACAAC AGTATTTCTG TAAAGCAGAG ATGTAAAAGA 
AAAATAGAAG CTACTGAAGT GATACAGTCG TATGCTAAAG AGGCAAAAAA AGGTCTCCAG 
GTGGATGTTT GTTCATCTGC TGAAGATGTC GATGAGGACA AAGACAAGGA TGAGACTGAA 
ACTGTTAAAC AAGTACAGGA CTCAGAAATG TATGATGGGA ATAGAGTTCC TGAAAGTATT 
AGGTCTGATG CATCTGATCA GGAGGAAGAT GATGAAAGTG AAAGCGGTCC AGTGGCAATA 
AGTTTATCAA AAGCAGAAAC CCAAGCTCTG ACTAACTATG GTAGTGGAGA AGATGAGAAT 
GAAGATGAAG AAATAGAATT TGAGGAAGGA CCTGTTGATG TGCAGACGTC ATTACAAGCC 
AGCAGTGAAA CAACAACTGA AAATGAACAG AATTCAAACC AAGAACAAAG TAAGACAAAA 
AGCAGTGAGA TTCTGTCATC AGAACAAGAA TCTGTTTATG CTAAAGGTGA ACAACATGTG 
GCTACAATTT TGCCTTATTA CCTCAATGTA ATGGAGAATG CACCGCCTTT AACGGTCAAT 
ACCCCAGAAT CTTTTATAAC AGACAGTATG AAAACAGAAG AATCAAGTTC ATCTTTACCA 
GTAAATGAAA CTCAAACACT GGATACAACG TGTGTAGGAA ACAAATCTGC TGCAAGTTCT 
GAAAGCTCCA TGGCTGGCAG CCCTGATACA GAGTCACCTG TGTTAGTGAA TGAATATGAA 
GCTGGTTCTG GAAATGTAAG TCAAAGATCT GATGAAGATG ACTTTGTGAA AGTTGAAGAC 
TTGCCCCTTA AACTTGCAGT GTATTCAGAG GCAGATTTAA TGAAGAAAAT GGCAATGGAG 
GCCCAAACGA ACAGTTTGTC TGATGAATTA CTGGGTGGAG GTGGAGCTCA AGATCAAGAA 
TTAGTAGGAG ATGCCCAAAC TTTGAAAGAA CCTGAAACTT TTGGAGTTCA AAGTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_021285858.1 ) cDNA ORF clone, Columba livia(rock pigeon) -> XP_021141533.1 Columba livia pericentriolar material 1 (PCM1), transcript variant X5, mRNA.

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CloneID	OCo293161
Clone ID Related Accession (Same CDS sequence)	XM_021285858.1
Accession Version	XM_021285858.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-23
Organism	Columba livia(rock pigeon)
Product	pericentriolar material 1 protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004973253.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Columba livia Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACAG GAGGTGGTCC CTTTGAAGAA GGCATGAATG ATCAAGACTT GCCTAGCTGG 
AGCAATGACA GCTTTGATGA CCGGCTGAAC AACACGGACT GGGGAAGTCA ACAGAAGAAA 
GCAAACAGAT CTTCAGAAAA AAACAAGAAA AAGCTTGGTG GGGAAGCTGA AACAAGGCTT 
ACTAATGATA TATCTCCAGA ATCCTCACCT GGAATAGGAC GACGAAAGAC CAGAACTCCT 
CATAGTTTTC CTCATGCTCG ATACATGACC CAGATGTCTG TTCCGGAGCA GGCTGAGCTA 
GAAAGGCTTA AACAAAGAAT AAACTTCAGT GATCTGGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGCA GGGCAACAGC TGCTAACAAC AAACGTCAAC TTAATGAAAA CAAAAAACCA 
TTCAACTTCC TGTCACTGCA GATAAACACT AACAAGAGCA AAGATCCTGC CTCATGTTCC 
CAAAAAAAGG AAAGTGGGGT GTCAGTCCAA CGTAAAGAGT TGTTTGGTGC TGCTCTGAGC 
AAGGATTTCT TGCAAAATTG TCAAGTCTCT GCTCAAGAAG ATGGAGGGGG AGAACCAGCT 
ATGGATAGTA GCCAGATTGT GAGCAGACTA GTTCAAATTC GCGACTATAT TGCTAAGGCC 
AGCTCCATGC GGGATGATCT TGTAGAGAAA AATGAAAGAT CGGCCAATGT TGAGCGTTTA 
TCACACCTTA TAGATCACCT TAAAGAGCAG GAGAAATCCT ATCTGAAATT TTTGCAAAAG 
ATGCTTGCTA GACAAAATGA GGAGGATGAT GTTCGGACTA TAGATTCAGC TGTGGGATCT 
GGTTCTGTAG GTGAGAGCAC ATCGCTAAAC ATTGATGTGC AGTCTGAGGC TTCAGATACC 
ACGGAGGTAT CTTTTAGTTT GAGCATTCGG CCCTGCATTG AGGACAAACT AGGGAATTCA 
GCTTCACAAG AACAGGTTAC AGACTTTGAT GTTACTGCAA GCCCTAAAGG GAAAAATGAC 
AGAGCTGCCC TGAATGACAG GGAAATCTGG CCTTGTAGGA TTAATAGCCG AGAACATGGA 
TTGCCTTCAA AGGCTAGAGA TCCTCAACAG GAGGCTAAAG AGGAGTTGGA GAACTTGAAG 
AAGCAGCATG ATTTATTGAA AAGGATGCTA AAACAGCAAG AGCAATTAAA GGCTCTTCAA 
GGAAGACAGG CAGCTCTTCT TGCTTTGCAG CATAAAGCAG AGCAAGCAAT TGCTGTCATG 
GATGATTCTG TTGTAACAGA GACTACAGGT AGTGTTTCAG GAGTAAGCCT TACATCAGAA 
CTGAATGAAG AATTGAATGA CTTAATTCAG CGCTTTCACA ACCAACTTCA TGATTCTCAG 
ACACAGTCTG TACCAGACAA TAGAAGGCAA GCAGAAAGTC TTTCACTTAC TAGAGAGGTT 
TCACAAAGCA GAAACTCTTC AATGTCTGAA CGCCTGTCAG ATGAGAAGGC GCAGCTTTTT 
AACAAGATGC GAATGTTGCA GGGTAAAAAG CAAAAAATGG ACAAACTGTT AGGAGAACTT 
CATACACTTC GTGACCAACA TCTAAATAAC TCCTCCTTTT TTCCTGCTTC AGGTTCTCCT 
CAAAGAAGTA TTGATCAAAG AAGTACAACT TCAGCTGCTT CTGGTCCTGC AGGCATAGTA 
ACTGTTGTCA ATGGCGAATC AAATAGCCTG GCATCTGCTC CCTATCCTCC TGATTCCCTG 
GTTTCTCAGA ATGAGAGTGA AGAAGATGAC AATCTAAATC CAACAGAAAA GCTTCAGAAA 
CTAAATGAAG TTCGTAAGAG GCTGAATGAG TTACGTGAGC TAGTTCACTA CTATGAGCAA 
ACATCTGATA TGATGACAGA TTCTGTGAAT GAAAACACTA AGGAAGAAGA AGAAACCGAA 
GAATCAGAAA GTGATTCTGA ACGTGAGGAT CCACAGCCTG TTACAAATAT TAGAAATCCT 
CAAGGAATCA GTAGTTGGAG TGACATAAAT AGCAACTCAA ATGTACAGTG TGGAACTAAT 
AACAGAGATG GAAGACATCT TAATACAGAC TGTGAAATAA ATAACAGATC TGCTGCTAAC 
ATAAGGACTC TAAAAATGTC GTCACCTTTA GACTGTCATA ATAGGGAGGA TGACAAAGAT 
ATTGGCCTAC CCCAATGTGA AGATGAAGAA GTGGAAGAAG ATAGAGTTAG TGAAGATTCT 
GCATCTAGTC ACAGAAGCAG CCTGGGTGAT ACTGCTGGAG ATGCTGAGTT TGAGCAGAAG 
ATCAATAGGC TTATAGCTGC AAAACAGAAA CTTAGACAAT TACAAAACCT TGCTGCTATG 
GTGCAGGATG ATGATCCAGC ACCTCAGACA ACAATTGCAA ATGCATCTAA TATTGGTGAC 
TTGTTTTTGG GTGAGATGGA AGAGACAAAG CAGCAGCCAA ACAATGTCCG AGCTAGTACC 
AACAAATTAC AAAAAGATGT AGAACTGAAT GAAAAAGCAA GAGAGAAGTT TTATGAAGCT 
AAACTCCAGC AGCAGCAACA GGAGCTTAAG CAGTTACAAG AAGAAAGAAG AAAACTGATT 
GAAATTCAAG AAAAAATTCA GGTGTTACAG AAAGCTTGTC CTGATCTTCA ATTGTCAGCT 
GGCTTGGGTG ACTGCACAGC AAATAGACAG ACTTCACCAG CAACATCAAC TCCAGCCATG 
AATGACACAG CTGGCAAGCC TTCACTTGAA TTTGATGAAT CTGTACCAGT AGGCAATGAG 
TTATGGTCTG AGATGAGAAG ACATGAGATT TTAAGAGAAG AATTGCGACA AAGAAGAAAG 
CAACTGGAAG CTCTAATGGC TGAGCATCAG AGGAGGAAAG AGCTCGCAGA AACAATATCT 
ACTGTTGCTG CGTCTGTTAA AAGTGAAGGA TCAGAAGCTC GGCGTACTCC ACAGCAGAGT 
AGGACTGAAA AGACAATGGC TACCTGGGGA GGTTCTACGC AGTGTGCACT AGATGAAGAG 
GATGGAGATG AAGATGGTTA TCTCTCTGAT GGAGCTGGTC AGGCAGAAGA AGATGATGAT 
GATGCGTCAA GTGCGAATGA CAGCTTCTCT GTTTATCCCA ATAACAACGT ACCAGAAAAT 
GCATATTTTG TTAAAGAAAA CAAGGATAGG TGGAAAAATT ACCGTCCTCT TTCAGCAGAT 
GGAAATTATC GTCCATTGTC TAAGGCCAGA CAACAGCAAA ACATAAGTAT GCGACGTCAG 
GAAAACCTTC GGTGGATGTC TGAACTTTCA TATGTGGAAG AAAAGGAACG ATGGCAAGAG 
CAGATCAATC AGTTGAAGAA ACAGCATGAA TTTAGTGTCA GCATTTGTCA AACTTTAATG 
CAGGATCAGC AGACCCTCTC TTGTCTTCTA CAGACTTTGC TTACAGGCCC TTACAGTATG 
ATGCCCAATA ATGTTGCATC TTCACAAGTA CATCTTATTA TGCATCAGCT AAACCAGTGT 
TACACTCAAC TGACATGGCA GCAGAATAAC GTCCAGAGGT TGAAACAAAT GTTAAATGAT 
CTTGTGCGTC AGCAAGAAGA ACAACGGTAT GAAGAGAAGT CATCAAGAAA GGATAGAGGC 
AGTGGTGTAC CCCCACCTCC ATCTCCTGTT TTCTGTCCGT TCAGCTTTCC TCCACAACCT 
TTGAACCTGT TTAATGTACC AGGATTTACT AATTTTTCTT CCTTTGCTCC AGATATTAAC 
TATAATCCAG TGTTTCCTTC TGGTTTTGGA GATTTTGCAC ACAATATTTC TCCACACAGC 
AGTGAGCAGC AGCAGCAACA TCCTCTAGAT CATAATGCAT CTGGGAAAAC TGAGTATATG 
GCGTTCCCCA AACCTTTTGA AAGCAGTTCC TCTAATGGAG CGGAAAAACA AAGGAGGAGT 
CACAGACAAC CTGAAGAGGA AATGGAAAAA AGATCAGCTT GGCTTAATGA TAGCCAGGAC 
GTGAAAAAAG ATGATCAGTC TCAGCTGAAA GCAAGTTTTG CTGTTTCAGT ACAAAACATT 
GCTTCCAATC ATAAAAATCA GTCTGATATG AGCAGAAGAA GAGAGTTTGA TGAAGAGTCT 
TTGGAGAGTT TTAGTAGCAT GCCTGATCCA GTAGACCCCA CTACTGTGAC AAAGACATTT 
AAATCTAGAA AAGCATCAGC ACAAGCAAGT TTGGCATCAA AAGATAAAAC ACCTAAATCG 
AAGAATAAGA GGAAGAGTTC TTCTCAGCTA AAAGGCAGAA TTACAAATAT TGATTATGAA 
AGTGCCAGTG CTTCTAGTGT GTGTGAACCC TGCAAGAGCA ATAAAAGCAG ACACTCTGAA 
GAGGTTGGTC ATGCAAAGGT GTTCAGCAAA AGGAATCGGG AACAACTGGA AAAAATCATT 
AAATACAGTA GATCTACAGA AATGTCTTCA GCGCATGCTA GGAGAATTCT GCAGCAGTCT 
AACAGAAATG CATGCATTGA AGCGCCAGAA ACTGGTAGTG ATCTTTCTAT GTTTGAAGCT 
TTACGAGACA CAATTTATTC TGAAGTGGCA ACTCTTATTT CTCAAAATGA GTCTCGTCCC 
CACTTTCTTA TTGAACTTTT CCATGAGCTT CAGCTGCTAA ATACAGATTA CCTGAGGCAA 
AGAGCTCTAT ACGCTCTACA GGATATAGTG ACCAGACATT TATCTGAGAA CAATGAAAAA 
GGGAAGTGCA CAAAATCACT GAATTCTGCA GCATGGATAG CATCAAATTC TGAACTCACT 
CCCAGTGAAA GCCTTGCTTC TACAGATGAT GAAACTTTTG GCAAAAACTT TTCTACAGAA 
GCATGTCAAG ATTGTGAACA AAATGATGCA GACAATGGGA GTAGCATGTC TACATCTTCA 
AATTTTGAAC CCTTTGCCAC TGATGACCTT GGCAACACAG TGATTCACTT AGATCAAGCT 
TTGGCTAGGA TGAGGGAGTA TGAGCGTATG AAAATTGAAG CTGAAAGTAC CCTTGACTCT 
GAGGGCTGCT CTAGTAATTT TCAGGGTGCT TCTGCTGCTA AATTAGAAGG AGGTCAAAGT 
GCCAGTGAAT GTGTTCCTGT GCCACAGTCA AGTGAAGCTT CTGCTGTTCC ATGTCCTCAT 
ATAGACACTC AGCAGCTTGA CCGGCAGATT AAAGCAATTA TGAAAGAGGT CATTCCTTTT 
CTGAAGGAAC ACATGGATGA AGTATGCTCT TCGCAGTTAC TGACATCAGT AAGACGTATG 
GTCTTGACCC TTACACAACA AAATGATGAA AGTAAAGAGT TTGTGAAGTT CTTTCATAAG 
CAGCTTGGCA GTATACTTCA GGATTCACTG GCAAAATTTG CTGGTAGAAA ACTGAAAGAT 
TGTGGAGAGG ATCTTCTAGT GGAGATCTCT GAAGTGTTAT TTAATGAATT AGCCTTTTTT 
AAACTCATGC AAGACTTGGA CAACAACAGT ATTTCTGTAA AGCAGAGATG TAAAAGAAAA 
ATAGAAGCTA CTGAAGTGAT ACAGTCGTAT GCTAAAGAGG CAAAAAAAGG TCTCCAGGTG 
GATGTTTGTT CATCTGCTGA AGATGTCGAT GAGGACAAAG ACAAGGATGA GACTGAAACT 
GTTAAACAAG TACAGGACTC AGAAATGTAT GATGGGAATA GAGTTCCTGA AAGTATTAGG 
TCTGATGCAT CTGATCAGGA GGAAGATGAT GAAAGTGAAA GCGGTCCAGT GGCAATAAGT 
TTATCAAAAG CAGAAACCCA AGCTCTGACT AACTATGGTA GTGGAGAAGA TGAGAATGAA 
GATGAAGAAA TAGAATTTGA GGAAGGACCT GTTGATGTGC AGACGTCATT ACAAGCCAGC 
AGTGAAACAA CAACTGAAAA TGAACAGAAT TCAAACCAAG AACAAAGTAA GACAAAAAGC 
AGTGAGATTC TGTCATCAGA ACAAGAATCT GTTTATGCTA AAGGTGAACA ACATGTGGCT 
ACAATTTTGC CTTATTACCT CAATGTAATG GAGAATGCAC CGCCTTTAAC GGTCAATACC 
CCAGAATCTT TTATAACAGA CAGTATGAAA ACAGAAGAAT CAAGTTCATC TTTACCAGTA 
AATGAAACTC AAACACTGGA TACAACGTGT GTAGGAAACA AATCTGCTGC AAGTTCTGAA 
AGCTCCATGG CTGGCAGCCC TGATACAGAG TCACCTGTGT TAGTGAATGA ATATGAAGCT 
GGTTCTGGAA ATGTAAGTCA AAGATCTGAT GAAGATGACT TTGTGAAAGT TGAAGACTTG 
CCCCTTAAAC TTGCAGTGTA TTCAGAGGCA GATTTAATGA AGAAAATGGC AATGGAGGCC 
CAAACGAACA GTTTGTCTGA TGAATTACTG GGTGGAGGTG GAGCTCAAGA TCAAGAATTA 
GTAGGAGATG CCCAAACTTT GAAAGAACCT GAAACTTTTG GAGTTCAAAG TGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021141533.1
CDS	140..6556
Translation	MATGGGPFEEGMNDQDLPSWSNDSFDDRLNNTDWGSQQKKANRSSEKNKKKLGGEAETRLTNDISPESSPGIGRRKTRTPHSFPHARYMTQMSVPEQAELERLKQRINFSDLDQRSIGSDSQGRATAANNKRQLNENKKPFNFLSLQINTNKSKDPASCSQKKESGVSVQRKELFGAALSKDFLQNCQVSAQEDGGGEPAMDSSQIVSRLVQIRDYIAKASSMRDDLVEKNERSANVERLSHLIDHLKEQEKSYLKFLQKMLARQNEEDDVRTIDSAVGSGSVGESTSLNIDVQSEASDTTEVSFSLSIRPCIEDKLGNSASQEQVTDFDVTASPKGKNDRAALNDREIWPCRINSREHGLPSKARDPQQEAKEELENLKKQHDLLKRMLKQQEQLKALQGRQAALLALQHKAEQAIAVMDDSVVTETTGSVSGVSLTSELNEELNDLIQRFHNQLHDSQTQSVPDNRRQAESLSLTREVSQSRNSSMSERLSDEKAQLFNKMRMLQGKKQKMDKLLGELHTLRDQHLNNSSFFPASGSPQRSIDQRSTTSAASGPAGIVTVVNGESNSLASAPYPPDSLVSQNESEEDDNLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDSVNENTKEEEETEESESDSEREDPQPVTNIRNPQGISSWSDINSNSNVQCGTNNRDGRHLNTDCEINNRSAANIRTLKMSSPLDCHNREDDKDIGLPQCEDEEVEEDRVSEDSASSHRSSLGDTAGDAEFEQKINRLIAAKQKLRQLQNLAAMVQDDDPAPQTTIANASNIGDLFLGEMEETKQQPNNVRASTNKLQKDVELNEKAREKFYEAKLQQQQQELKQLQEERRKLIEIQEKIQVLQKACPDLQLSAGLGDCTANRQTSPATSTPAMNDTAGKPSLEFDESVPVGNELWSEMRRHEILREELRQRRKQLEALMAEHQRRKELAETISTVAASVKSEGSEARRTPQQSRTEKTMATWGGSTQCALDEEDGDEDGYLSDGAGQAEEDDDDASSANDSFSVYPNNNVPENAYFVKENKDRWKNYRPLSADGNYRPLSKARQQQNISMRRQENLRWMSELSYVEEKERWQEQINQLKKQHEFSVSICQTLMQDQQTLSCLLQTLLTGPYSMMPNNVASSQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNDLVRQQEEQRYEEKSSRKDRGSGVPPPPSPVFCPFSFPPQPLNLFNVPGFTNFSSFAPDINYNPVFPSGFGDFAHNISPHSSEQQQQHPLDHNASGKTEYMAFPKPFESSSSNGAEKQRRSHRQPEEEMEKRSAWLNDSQDVKKDDQSQLKASFAVSVQNIASNHKNQSDMSRRREFDEESLESFSSMPDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKNKRKSSSQLKGRITNIDYESASASSVCEPCKSNKSRHSEEVGHAKVFSKRNREQLEKIIKYSRSTEMSSAHARRILQQSNRNACIEAPETGSDLSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHLSENNEKGKCTKSLNSAAWIASNSELTPSESLASTDDETFGKNFSTEACQDCEQNDADNGSSMSTSSNFEPFATDDLGNTVIHLDQALARMREYERMKIEAESTLDSEGCSSNFQGASAAKLEGGQSASECVPVPQSSEASAVPCPHIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSISVKQRCKRKIEATEVIQSYAKEAKKGLQVDVCSSAEDVDEDKDKDETETVKQVQDSEMYDGNRVPESIRSDASDQEEDDESESGPVAISLSKAETQALTNYGSGEDENEDEEIEFEEGPVDVQTSLQASSETTTENEQNSNQEQSKTKSSEILSSEQESVYAKGEQHVATILPYYLNVMENAPPLTVNTPESFITDSMKTEESSSSLPVNETQTLDTTCVGNKSAASSESSMAGSPDTESPVLVNEYEAGSGNVSQRSDEDDFVKVEDLPLKLAVYSEADLMKKMAMEAQTNSLSDELLGGGGAQDQELVGDAQTLKEPETFGVQSA

Target ORF information:

RefSeq Version	XM_021285858.1
Organism	Columba livia(rock pigeon)
Definition	Columba livia pericentriolar material 1 (PCM1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021285858.1

ORF Insert Sequence:

ATGGCAACAG GAGGTGGTCC CTTTGAAGAA GGCATGAATG ATCAAGACTT GCCTAGCTGG 
AGCAATGACA GCTTTGATGA CCGGCTGAAC AACACGGACT GGGGAAGTCA ACAGAAGAAA 
GCAAACAGAT CTTCAGAAAA AAACAAGAAA AAGCTTGGTG GGGAAGCTGA AACAAGGCTT 
ACTAATGATA TATCTCCAGA ATCCTCACCT GGAATAGGAC GACGAAAGAC CAGAACTCCT 
CATAGTTTTC CTCATGCTCG ATACATGACC CAGATGTCTG TTCCGGAGCA GGCTGAGCTA 
GAAAGGCTTA AACAAAGAAT AAACTTCAGT GATCTGGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGCA GGGCAACAGC TGCTAACAAC AAACGTCAAC TTAATGAAAA CAAAAAACCA 
TTCAACTTCC TGTCACTGCA GATAAACACT AACAAGAGCA AAGATCCTGC CTCATGTTCC 
CAAAAAAAGG AAAGTGGGGT GTCAGTCCAA CGTAAAGAGT TGTTTGGTGC TGCTCTGAGC 
AAGGATTTCT TGCAAAATTG TCAAGTCTCT GCTCAAGAAG ATGGAGGGGG AGAACCAGCT 
ATGGATAGTA GCCAGATTGT GAGCAGACTA GTTCAAATTC GCGACTATAT TGCTAAGGCC 
AGCTCCATGC GGGATGATCT TGTAGAGAAA AATGAAAGAT CGGCCAATGT TGAGCGTTTA 
TCACACCTTA TAGATCACCT TAAAGAGCAG GAGAAATCCT ATCTGAAATT TTTGCAAAAG 
ATGCTTGCTA GACAAAATGA GGAGGATGAT GTTCGGACTA TAGATTCAGC TGTGGGATCT 
GGTTCTGTAG GTGAGAGCAC ATCGCTAAAC ATTGATGTGC AGTCTGAGGC TTCAGATACC 
ACGGAGGTAT CTTTTAGTTT GAGCATTCGG CCCTGCATTG AGGACAAACT AGGGAATTCA 
GCTTCACAAG AACAGGTTAC AGACTTTGAT GTTACTGCAA GCCCTAAAGG GAAAAATGAC 
AGAGCTGCCC TGAATGACAG GGAAATCTGG CCTTGTAGGA TTAATAGCCG AGAACATGGA 
TTGCCTTCAA AGGCTAGAGA TCCTCAACAG GAGGCTAAAG AGGAGTTGGA GAACTTGAAG 
AAGCAGCATG ATTTATTGAA AAGGATGCTA AAACAGCAAG AGCAATTAAA GGCTCTTCAA 
GGAAGACAGG CAGCTCTTCT TGCTTTGCAG CATAAAGCAG AGCAAGCAAT TGCTGTCATG 
GATGATTCTG TTGTAACAGA GACTACAGGT AGTGTTTCAG GAGTAAGCCT TACATCAGAA 
CTGAATGAAG AATTGAATGA CTTAATTCAG CGCTTTCACA ACCAACTTCA TGATTCTCAG 
ACACAGTCTG TACCAGACAA TAGAAGGCAA GCAGAAAGTC TTTCACTTAC TAGAGAGGTT 
TCACAAAGCA GAAACTCTTC AATGTCTGAA CGCCTGTCAG ATGAGAAGGC GCAGCTTTTT 
AACAAGATGC GAATGTTGCA GGGTAAAAAG CAAAAAATGG ACAAACTGTT AGGAGAACTT 
CATACACTTC GTGACCAACA TCTAAATAAC TCCTCCTTTT TTCCTGCTTC AGGTTCTCCT 
CAAAGAAGTA TTGATCAAAG AAGTACAACT TCAGCTGCTT CTGGTCCTGC AGGCATAGTA 
ACTGTTGTCA ATGGCGAATC AAATAGCCTG GCATCTGCTC CCTATCCTCC TGATTCCCTG 
GTTTCTCAGA ATGAGAGTGA AGAAGATGAC AATCTAAATC CAACAGAAAA GCTTCAGAAA 
CTAAATGAAG TTCGTAAGAG GCTGAATGAG TTACGTGAGC TAGTTCACTA CTATGAGCAA 
ACATCTGATA TGATGACAGA TTCTGTGAAT GAAAACACTA AGGAAGAAGA AGAAACCGAA 
GAATCAGAAA GTGATTCTGA ACGTGAGGAT CCACAGCCTG TTACAAATAT TAGAAATCCT 
CAAGGAATCA GTAGTTGGAG TGACATAAAT AGCAACTCAA ATGTACAGTG TGGAACTAAT 
AACAGAGATG GAAGACATCT TAATACAGAC TGTGAAATAA ATAACAGATC TGCTGCTAAC 
ATAAGGACTC TAAAAATGTC GTCACCTTTA GACTGTCATA ATAGGGAGGA TGACAAAGAT 
ATTGGCCTAC CCCAATGTGA AGATGAAGAA GTGGAAGAAG ATAGAGTTAG TGAAGATTCT 
GCATCTAGTC ACAGAAGCAG CCTGGGTGAT ACTGCTGGAG ATGCTGAGTT TGAGCAGAAG 
ATCAATAGGC TTATAGCTGC AAAACAGAAA CTTAGACAAT TACAAAACCT TGCTGCTATG 
GTGCAGGATG ATGATCCAGC ACCTCAGACA ACAATTGCAA ATGCATCTAA TATTGGTGAC 
TTGTTTTTGG GTGAGATGGA AGAGACAAAG CAGCAGCCAA ACAATGTCCG AGCTAGTACC 
AACAAATTAC AAAAAGATGT AGAACTGAAT GAAAAAGCAA GAGAGAAGTT TTATGAAGCT 
AAACTCCAGC AGCAGCAACA GGAGCTTAAG CAGTTACAAG AAGAAAGAAG AAAACTGATT 
GAAATTCAAG AAAAAATTCA GGTGTTACAG AAAGCTTGTC CTGATCTTCA ATTGTCAGCT 
GGCTTGGGTG ACTGCACAGC AAATAGACAG ACTTCACCAG CAACATCAAC TCCAGCCATG 
AATGACACAG CTGGCAAGCC TTCACTTGAA TTTGATGAAT CTGTACCAGT AGGCAATGAG 
TTATGGTCTG AGATGAGAAG ACATGAGATT TTAAGAGAAG AATTGCGACA AAGAAGAAAG 
CAACTGGAAG CTCTAATGGC TGAGCATCAG AGGAGGAAAG AGCTCGCAGA AACAATATCT 
ACTGTTGCTG CGTCTGTTAA AAGTGAAGGA TCAGAAGCTC GGCGTACTCC ACAGCAGAGT 
AGGACTGAAA AGACAATGGC TACCTGGGGA GGTTCTACGC AGTGTGCACT AGATGAAGAG 
GATGGAGATG AAGATGGTTA TCTCTCTGAT GGAGCTGGTC AGGCAGAAGA AGATGATGAT 
GATGCGTCAA GTGCGAATGA CAGCTTCTCT GTTTATCCCA ATAACAACGT ACCAGAAAAT 
GCATATTTTG TTAAAGAAAA CAAGGATAGG TGGAAAAATT ACCGTCCTCT TTCAGCAGAT 
GGAAATTATC GTCCATTGTC TAAGGCCAGA CAACAGCAAA ACATAAGTAT GCGACGTCAG 
GAAAACCTTC GGTGGATGTC TGAACTTTCA TATGTGGAAG AAAAGGAACG ATGGCAAGAG 
CAGATCAATC AGTTGAAGAA ACAGCATGAA TTTAGTGTCA GCATTTGTCA AACTTTAATG 
CAGGATCAGC AGACCCTCTC TTGTCTTCTA CAGACTTTGC TTACAGGCCC TTACAGTATG 
ATGCCCAATA ATGTTGCATC TTCACAAGTA CATCTTATTA TGCATCAGCT AAACCAGTGT 
TACACTCAAC TGACATGGCA GCAGAATAAC GTCCAGAGGT TGAAACAAAT GTTAAATGAT 
CTTGTGCGTC AGCAAGAAGA ACAACGGTAT GAAGAGAAGT CATCAAGAAA GGATAGAGGC 
AGTGGTGTAC CCCCACCTCC ATCTCCTGTT TTCTGTCCGT TCAGCTTTCC TCCACAACCT 
TTGAACCTGT TTAATGTACC AGGATTTACT AATTTTTCTT CCTTTGCTCC AGATATTAAC 
TATAATCCAG TGTTTCCTTC TGGTTTTGGA GATTTTGCAC ACAATATTTC TCCACACAGC 
AGTGAGCAGC AGCAGCAACA TCCTCTAGAT CATAATGCAT CTGGGAAAAC TGAGTATATG 
GCGTTCCCCA AACCTTTTGA AAGCAGTTCC TCTAATGGAG CGGAAAAACA AAGGAGGAGT 
CACAGACAAC CTGAAGAGGA AATGGAAAAA AGATCAGCTT GGCTTAATGA TAGCCAGGAC 
GTGAAAAAAG ATGATCAGTC TCAGCTGAAA GCAAGTTTTG CTGTTTCAGT ACAAAACATT 
GCTTCCAATC ATAAAAATCA GTCTGATATG AGCAGAAGAA GAGAGTTTGA TGAAGAGTCT 
TTGGAGAGTT TTAGTAGCAT GCCTGATCCA GTAGACCCCA CTACTGTGAC AAAGACATTT 
AAATCTAGAA AAGCATCAGC ACAAGCAAGT TTGGCATCAA AAGATAAAAC ACCTAAATCG 
AAGAATAAGA GGAAGAGTTC TTCTCAGCTA AAAGGCAGAA TTACAAATAT TGATTATGAA 
AGTGCCAGTG CTTCTAGTGT GTGTGAACCC TGCAAGAGCA ATAAAAGCAG ACACTCTGAA 
GAGGTTGGTC ATGCAAAGGT GTTCAGCAAA AGGAATCGGG AACAACTGGA AAAAATCATT 
AAATACAGTA GATCTACAGA AATGTCTTCA GCGCATGCTA GGAGAATTCT GCAGCAGTCT 
AACAGAAATG CATGCATTGA AGCGCCAGAA ACTGGTAGTG ATCTTTCTAT GTTTGAAGCT 
TTACGAGACA CAATTTATTC TGAAGTGGCA ACTCTTATTT CTCAAAATGA GTCTCGTCCC 
CACTTTCTTA TTGAACTTTT CCATGAGCTT CAGCTGCTAA ATACAGATTA CCTGAGGCAA 
AGAGCTCTAT ACGCTCTACA GGATATAGTG ACCAGACATT TATCTGAGAA CAATGAAAAA 
GGGAAGTGCA CAAAATCACT GAATTCTGCA GCATGGATAG CATCAAATTC TGAACTCACT 
CCCAGTGAAA GCCTTGCTTC TACAGATGAT GAAACTTTTG GCAAAAACTT TTCTACAGAA 
GCATGTCAAG ATTGTGAACA AAATGATGCA GACAATGGGA GTAGCATGTC TACATCTTCA 
AATTTTGAAC CCTTTGCCAC TGATGACCTT GGCAACACAG TGATTCACTT AGATCAAGCT 
TTGGCTAGGA TGAGGGAGTA TGAGCGTATG AAAATTGAAG CTGAAAGTAC CCTTGACTCT 
GAGGGCTGCT CTAGTAATTT TCAGGGTGCT TCTGCTGCTA AATTAGAAGG AGGTCAAAGT 
GCCAGTGAAT GTGTTCCTGT GCCACAGTCA AGTGAAGCTT CTGCTGTTCC ATGTCCTCAT 
ATAGACACTC AGCAGCTTGA CCGGCAGATT AAAGCAATTA TGAAAGAGGT CATTCCTTTT 
CTGAAGGAAC ACATGGATGA AGTATGCTCT TCGCAGTTAC TGACATCAGT AAGACGTATG 
GTCTTGACCC TTACACAACA AAATGATGAA AGTAAAGAGT TTGTGAAGTT CTTTCATAAG 
CAGCTTGGCA GTATACTTCA GGATTCACTG GCAAAATTTG CTGGTAGAAA ACTGAAAGAT 
TGTGGAGAGG ATCTTCTAGT GGAGATCTCT GAAGTGTTAT TTAATGAATT AGCCTTTTTT 
AAACTCATGC AAGACTTGGA CAACAACAGT ATTTCTGTAA AGCAGAGATG TAAAAGAAAA 
ATAGAAGCTA CTGAAGTGAT ACAGTCGTAT GCTAAAGAGG CAAAAAAAGG TCTCCAGGTG 
GATGTTTGTT CATCTGCTGA AGATGTCGAT GAGGACAAAG ACAAGGATGA GACTGAAACT 
GTTAAACAAG TACAGGACTC AGAAATGTAT GATGGGAATA GAGTTCCTGA AAGTATTAGG 
TCTGATGCAT CTGATCAGGA GGAAGATGAT GAAAGTGAAA GCGGTCCAGT GGCAATAAGT 
TTATCAAAAG CAGAAACCCA AGCTCTGACT AACTATGGTA GTGGAGAAGA TGAGAATGAA 
GATGAAGAAA TAGAATTTGA GGAAGGACCT GTTGATGTGC AGACGTCATT ACAAGCCAGC 
AGTGAAACAA CAACTGAAAA TGAACAGAAT TCAAACCAAG AACAAAGTAA GACAAAAAGC 
AGTGAGATTC TGTCATCAGA ACAAGAATCT GTTTATGCTA AAGGTGAACA ACATGTGGCT 
ACAATTTTGC CTTATTACCT CAATGTAATG GAGAATGCAC CGCCTTTAAC GGTCAATACC 
CCAGAATCTT TTATAACAGA CAGTATGAAA ACAGAAGAAT CAAGTTCATC TTTACCAGTA 
AATGAAACTC AAACACTGGA TACAACGTGT GTAGGAAACA AATCTGCTGC AAGTTCTGAA 
AGCTCCATGG CTGGCAGCCC TGATACAGAG TCACCTGTGT TAGTGAATGA ATATGAAGCT 
GGTTCTGGAA ATGTAAGTCA AAGATCTGAT GAAGATGACT TTGTGAAAGT TGAAGACTTG 
CCCCTTAAAC TTGCAGTGTA TTCAGAGGCA GATTTAATGA AGAAAATGGC AATGGAGGCC 
CAAACGAACA GTTTGTCTGA TGAATTACTG GGTGGAGGTG GAGCTCAAGA TCAAGAATTA 
GTAGGAGATG CCCAAACTTT GAAAGAACCT GAAACTTTTG GAGTTCAAAG TGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_021285859.1 ) cDNA ORF clone, Columba livia(rock pigeon) -> XP_021141534.1 Columba livia pericentriolar material 1 (PCM1), transcript variant X6, mRNA.

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CloneID	OCo293162
Clone ID Related Accession (Same CDS sequence)	XM_021285859.1
Accession Version	XM_021285859.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-23
Organism	Columba livia(rock pigeon)
Product	pericentriolar material 1 protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004973253.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Columba livia Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACAG GAGGTGGTCC CTTTGAAGAA GGCATGAATG ATCAAGACTT GCCTAGCTGG 
AGCAATGACA GCTTTGATGA CCGGCTGAAC AACACGGACT GGGGAAGTCA ACAGAAGAAA 
GCAAACAGAT CTTCAGAAAA AAACAAGAAA AAGCTTGGTG GGGAAGCTGA AACAAGGCTT 
ACTAATGATA TATCTCCAGA ATCCTCACCT GGAATAGGAC GACGAAAGAC CAGAACTCCT 
CATAGTTTTC CTCATGCTCG ATACATGACC CAGATGTCTG TTCCGGAGCA GGCTGAGCTA 
GAAAGGCTTA AACAAAGAAT AAACTTCAGT GATCTGGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGCA GGGCAACAGC TGCTAACAAC AAACGTCAAC TTAATGAAAA CAAAAAACCA 
TTCAACTTCC TGTCACTGCA GATAAACACT AACAAGAGCA AAGATCCTGC CTCATGTTCC 
CAAAAAAAGG AAAGTGGGGT GTCAGTCCAA CGTAAAGAGT TGTTTGGTGC TGCTCTGAGC 
AAGGATTTCT TGCAAAATTG TCAAGTCTCT GCTCAAGAAG ATGGAGGGGG AGAACCAGCT 
ATGGATAGTA GCCAGATTGT GAGCAGACTA GTTCAAATTC GCGACTATAT TGCTAAGGCC 
AGCTCCATGC GGGATGATCT TGTAGAGAAA AATGAAAGAT CGGCCAATGT TGAGCGTTTA 
TCACACCTTA TAGATCACCT TAAAGAGCAG GAGAAATCCT ATCTGAAATT TTTGCAAAAG 
ATGCTTGCTA GACAAAATGA GGAGGATGAT GTTCGGACTA TAGATTCAGC TGTGGGATCT 
GGTTCTGTAG GTGAGAGCAC ATCGCTAAAC ATTGATGTGC AGTCTGAGGC TTCAGATACC 
ACGGAGGTAT CTTTTAGTTT GAGCATTCGG CCCTGCATTG AGGACAAACT AGGGAATTCA 
GCTTCACAAG AACAGGTTAC AGACTTTGAT GTTACTGCAA GCCCTAAAGG GAAAAATGAC 
AGAGCTGCCC TGAATGACAG GGAAATCTGG CCTTGTAGGA TTAATAGCCG AGAACATGGA 
TTGCCTTCAA AGGCTAGAGA TCCTCAACAG GAGGCTAAAG AGGAGTTGGA GAACTTGAAG 
AAGCAGCATG ATTTATTGAA AAGGATGCTA AAACAGCAAG AGCAATTAAA GGCTCTTCAA 
GGAAGACAGG CAGCTCTTCT TGCTTTGCAG CATAAAGCAG AGCAAGCAAT TGCTGTCATG 
GATGATTCTG TTGTAACAGA GACTACAGGT AGTGTTTCAG GAGTAAGCCT TACATCAGAA 
CTGAATGAAG AATTGAATGA CTTAATTCAG CGCTTTCACA ACCAACTTCA TGATTCTCAG 
ACACAGTCTG TACCAGACAA TAGAAGGCAA GCAGAAAGTC TTTCACTTAC TAGAGAGGTT 
TCACAAAGCA GAAACTCTTC AATGTCTGAA CGCCTGTCAG ATGAGAAGGC GCAGCTTTTT 
AACAAGATGC GAATGTTGCA GGGTAAAAAG CAAAAAATGG ACAAACTGTT AGGAGAACTT 
CATACACTTC GTGACCAACA TCTAAATAAC TCCTCCTTTT TTCCTGCTTC AGGTTCTCCT 
CAAAGAAGTA TTGATCAAAG AAGTACAACT TCAGCTGCTT CTGGTCCTGC AGGCATAGTA 
ACTGTTGTCA ATGGCGAATC AAATAGCCTG GCATCTGCTC CCTATCCTCC TGATTCCCTG 
GTTTCTCAGA ATGAGAGTGA AGAAGATGAC AATCTAAATC CAACAGAAAA GCTTCAGAAA 
CTAAATGAAG TTCGTAAGAG GCTGAATGAG TTACGTGAGC TAGTTCACTA CTATGAGCAA 
ACATCTGATA TGATGACAGA TTCTGTGAAT GAAAACACTA AGGAAGAAGA AGAAACCGAA 
GAATCAGAAA GTGATTCTGA ACGTGAGGAT CCACAGCCTG TTACAAATAT TAGAAATCCT 
CAAGGAATCA GTAGTTGGAG TGACATAAAT AGCAACTCAA ATGTACAGTG TGGAACTAAT 
AACAGAGATG GAAGACATCT TAATACAGAC TGTGAAATAA ATAACAGATC TGCTGCTAAC 
ATAAGGACTC TAAAAATGTC GTCACCTTTA GACTGTCATA ATAGGGAGGA TGACAAAGAT 
ATTGGCCTAC CCCAATGTGA AGATGAAGAA GTGGAAGAAG ATAGAGTTAG TGAAGATTCT 
GCATCTAGTC ACAGAAGCAG CCTGGGTGAT ACTGCTGGAG ATGCTGAGTT TGAGCAGAAG 
ATCAATAGGC TTATAGCTGC AAAACAGAAA CTTAGACAAT TACAAAACCT TGCTGCTATG 
GTGCAGGATG ATGATCCAGC ACCTCAGACA ACAATTGCAA ATGCATCTAA TATTGGTGAC 
TTGTTTTTGG GTGAGATGGA AGAGACAAAG CAGCAGCCAA ACAATGTCCG AGCTAGTACC 
AACAAATTAC AAAAAGATGT AGAACTGAAT GAAAAAGCAA GAGAGAAGTT TTATGAAGCT 
AAACTCCAGC AGCAGCAACA GGAGCTTAAG CAGTTACAAG AAGAAAGAAG AAAACTGATT 
GAAATTCAAG AAAAAATTCA GGTGTTACAG AAAGCTTGTC CTGATCTTCA ATTGTCAGCT 
GGCTTGGGTG ACTGCACAGC AAATAGACAG ACTTCACCAG CAACATCAAC TCCAGCCATG 
AATGACACAG CTGGCAAGCC TTCACTTGAA TTTGATGAAT CTGTACCAGT AGGCAATGAG 
TTATGGTCTG AGATGAGAAG ACATGAGATT TTAAGAGAAG AATTGCGACA AAGAAGAAAG 
CAACTGGAAG CTCTAATGGC TGAGCATCAG AGGAGGAAAG AGCTCGCAGA AACAATATCT 
ACTGTTGCTG CGTCTGTTAA AAGTGAAGGA TCAGAAGCTC GGCGTACTCC ACAGCAGAGT 
AGGACTGAAA ATAGGACAAT GGCTACCTGG GGAGGTTCTA CGCAGTGTGC ACTAGATGAA 
GAGGATGGAG ATGAAGATGG TTATCTCTCT GATGGAGCTG GTCAGGCAGA AGAAGATGAT 
GATGATGCGT CAAGTGCGAA TGACAGCTTC TCTGTTTATC CCAATAACAA CGTACCAGAA 
AATGCATATT TTGTTAAAGA AAACAAGGAT AGGTGGAAAA ATTACCGTCC TCTTTCAGCA 
GATGGAAATT ATCGTCCATT GTCTAAGGCC AGACAACAGC AAAACATAAG TATGCGACGT 
CAGGAAAACC TTCGGTGGAT GTCTGAACTT TCATATGTGG AAGAAAAGGA ACGATGGCAA 
GAGCAGATCA ATCAGTTGAA GAAACAGCAT GAATTTAGTG TCAGCATTTG TCAAACTTTA 
ATGCAGGATC AGCAGACCCT CTCTTGTCTT CTACAGACTT TGCTTACAGG CCCTTACAGT 
ATGATGCCCA ATAATGTTGC ATCTTCACAA GTACATCTTA TTATGCATCA GCTAAACCAG 
TGTTACACTC AACTGACATG GCAGCAGAAT AACGTCCAGA GGTTGAAACA AATGTTAAAT 
GATCTTGTGC GTCAGCAAGA AGAACAACGG TATGAAGAGA AGTCATCAAG AAAGGATAGA 
GGCAGTGGTG TACCCCCACC TCCATCTCCT GTTTTCTGTC CGTTCAGCTT TCCTCCACAA 
CCTTTGAACC TGTTTAATGT ACCAGGATTT ACTAATTTTT CTTCCTTTGC TCCAGATATT 
AACTATAATC CAGTGTTTCC TTCTGGTTTT GGAGATTTTG CACACAATAT TTCTCCACAC 
AGCAGTGAGC AGCAGCAGCA ACATCCTCTA GATCATAATG CATCTGGGAA AACTGAGTAT 
ATGGCGTTCC CCAAACCTTT TGAAAGCAGT TCCTCTAATG GAGCGGAAAA ACAAAGGAGG 
AGTCACAGAC AACCTGAAGA GGAAATGGAA AAAAGATCAG CTTGGCTTAA TGATAGCCAG 
GACGTGAAAA AAGATGATCA GTCTCAGCTG AAAGCAAGTT TTGCTGTTTC AGTACAAAAC 
ATTGCTTCCA ATCATAAAAA TCAGTCTGAT ATGAGCAGAA GAAGAGAGTT TGATGAAGAG 
TCTTTGGAGA GTTTTAGTAG CATGCCTGAT CCAGTAGACC CCACTACTGT GACAAAGACA 
TTTAAATCTA GAAAAGCATC AGCACAAGCA AGTTTGGCAT CAAAAGATAA AACACCTAAA 
TCGAAGAATA AGAGGAAGAG TTCTTCTCAG CTAAAAGGCA GAATTACAAA TATTGATTAT 
GAAAGTGCCA GTGCTTCTAG TGTGTGTGAA CCCTGCAAGA GCAATAAAAG CAGACACTCT 
GAAGAGGTTG GTCATGCAAA GGTGTTCAGC AAAAGGAATC GGGAACAACT GGAAAAAATC 
ATTAAATACA GTAGATCTAC AGAAATGTCT TCAGAAACTG GTAGTGATCT TTCTATGTTT 
GAAGCTTTAC GAGACACAAT TTATTCTGAA GTGGCAACTC TTATTTCTCA AAATGAGTCT 
CGTCCCCACT TTCTTATTGA ACTTTTCCAT GAGCTTCAGC TGCTAAATAC AGATTACCTG 
AGGCAAAGAG CTCTATACGC TCTACAGGAT ATAGTGACCA GACATTTATC TGAGAACAAT 
GAAAAAGGGA AGTGCACAAA ATCACTGAAT TCTGCAGCAT GGATAGCATC AAATTCTGAA 
CTCACTCCCA GTGAAAGCCT TGCTTCTACA GATGATGAAA CTTTTGGCAA AAACTTTTCT 
ACAGAAGCAT GTCAAGATTG TGAACAAAAT GATGCAGACA ATGGGAGTAG CATGTCTACA 
TCTTCAAATT TTGAACCCTT TGCCACTGAT GACCTTGGCA ACACAGTGAT TCACTTAGAT 
CAAGCTTTGG CTAGGATGAG GGAGTATGAG CGTATGAAAA TTGAAGCTGA AAGTACCCTT 
GACTCTGAGG GCTGCTCTAG TAATTTTCAG GGTGCTTCTG CTGCTAAATT AGAAGGAGGT 
CAAAGTGCCA GTGAATGTGT TCCTGTGCCA CAGTCAAGTG AAGCTTCTGC TGTTCCATGT 
CCTCATATAG ACACTCAGCA GCTTGACCGG CAGATTAAAG CAATTATGAA AGAGGTCATT 
CCTTTTCTGA AGGAACACAT GGATGAAGTA TGCTCTTCGC AGTTACTGAC ATCAGTAAGA 
CGTATGGTCT TGACCCTTAC ACAACAAAAT GATGAAAGTA AAGAGTTTGT GAAGTTCTTT 
CATAAGCAGC TTGGCAGTAT ACTTCAGGAT TCACTGGCAA AATTTGCTGG TAGAAAACTG 
AAAGATTGTG GAGAGGATCT TCTAGTGGAG ATCTCTGAAG TGTTATTTAA TGAATTAGCC 
TTTTTTAAAC TCATGCAAGA CTTGGACAAC AACAGTATTT CTGTAAAGCA GAGATGTAAA 
AGAAAAATAG AAGCTACTGA AGTGATACAG TCGTATGCTA AAGAGGCAAA AAAAGGTCTC 
CAGGTGGATG TTTGTTCATC TGCTGAAGAT GTCGATGAGG ACAAAGACAA GGATGAGACT 
GAAACTGTTA AACAAGTACA GGACTCAGAA ATGTATGATG GGAATAGAGT TCCTGAAAGT 
ATTAGGTCTG ATGCATCTGA TCAGGAGGAA GATGATGAAA GTGAAAGCGG TCCAGTGGCA 
ATAAGTTTAT CAAAAGCAGA AACCCAAGCT CTGACTAACT ATGGTAGTGG AGAAGATGAG 
AATGAAGATG AAGAAATAGA ATTTGAGGAA GGACCTGTTG ATGTGCAGAC GTCATTACAA 
GCCAGCAGTG AAACAACAAC TGAAAATGAA CAGAATTCAA ACCAAGAACA AAGTAAGACA 
AAAAGCAGTG AGATTCTGTC ATCAGAACAA GAATCTGTTT ATGCTAAAGG TGAACAACAT 
GTGGCTACAA TTTTGCCTTA TTACCTCAAT GTAATGGAGA ATGCACCGCC TTTAACGGTC 
AATACCCCAG AATCTTTTAT AACAGACAGT ATGAAAACAG AAGAATCAAG TTCATCTTTA 
CCAGTAAATG AAACTCAAAC ACTGGATACA ACGTGTGTAG GAAACAAATC TGCTGCAAGT 
TCTGAAAGCT CCATGGCTGG CAGCCCTGAT ACAGAGTCAC CTGTGTTAGT GAATGAATAT 
GAAGCTGGTT CTGGAAATGT AAGTCAAAGA TCTGATGAAG ATGACTTTGT GAAAGTTGAA 
GACTTGCCCC TTAAACTTGC AGTGTATTCA GAGGCAGATT TAATGAAGAA AATGGCAATG 
GAGGCCCAAA CGAACAGTTT GTCTGATGAA TTACTGGGTG GAGGTGGAGC TCAAGATCAA 
GAATTAGTAG GAGATGCCCA AACTTTGAAA GAACCTGAAA CTTTTGGAGT TCAAAGTGCG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021141534.1
CDS	140..6502
Translation	MATGGGPFEEGMNDQDLPSWSNDSFDDRLNNTDWGSQQKKANRSSEKNKKKLGGEAETRLTNDISPESSPGIGRRKTRTPHSFPHARYMTQMSVPEQAELERLKQRINFSDLDQRSIGSDSQGRATAANNKRQLNENKKPFNFLSLQINTNKSKDPASCSQKKESGVSVQRKELFGAALSKDFLQNCQVSAQEDGGGEPAMDSSQIVSRLVQIRDYIAKASSMRDDLVEKNERSANVERLSHLIDHLKEQEKSYLKFLQKMLARQNEEDDVRTIDSAVGSGSVGESTSLNIDVQSEASDTTEVSFSLSIRPCIEDKLGNSASQEQVTDFDVTASPKGKNDRAALNDREIWPCRINSREHGLPSKARDPQQEAKEELENLKKQHDLLKRMLKQQEQLKALQGRQAALLALQHKAEQAIAVMDDSVVTETTGSVSGVSLTSELNEELNDLIQRFHNQLHDSQTQSVPDNRRQAESLSLTREVSQSRNSSMSERLSDEKAQLFNKMRMLQGKKQKMDKLLGELHTLRDQHLNNSSFFPASGSPQRSIDQRSTTSAASGPAGIVTVVNGESNSLASAPYPPDSLVSQNESEEDDNLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDSVNENTKEEEETEESESDSEREDPQPVTNIRNPQGISSWSDINSNSNVQCGTNNRDGRHLNTDCEINNRSAANIRTLKMSSPLDCHNREDDKDIGLPQCEDEEVEEDRVSEDSASSHRSSLGDTAGDAEFEQKINRLIAAKQKLRQLQNLAAMVQDDDPAPQTTIANASNIGDLFLGEMEETKQQPNNVRASTNKLQKDVELNEKAREKFYEAKLQQQQQELKQLQEERRKLIEIQEKIQVLQKACPDLQLSAGLGDCTANRQTSPATSTPAMNDTAGKPSLEFDESVPVGNELWSEMRRHEILREELRQRRKQLEALMAEHQRRKELAETISTVAASVKSEGSEARRTPQQSRTENRTMATWGGSTQCALDEEDGDEDGYLSDGAGQAEEDDDDASSANDSFSVYPNNNVPENAYFVKENKDRWKNYRPLSADGNYRPLSKARQQQNISMRRQENLRWMSELSYVEEKERWQEQINQLKKQHEFSVSICQTLMQDQQTLSCLLQTLLTGPYSMMPNNVASSQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNDLVRQQEEQRYEEKSSRKDRGSGVPPPPSPVFCPFSFPPQPLNLFNVPGFTNFSSFAPDINYNPVFPSGFGDFAHNISPHSSEQQQQHPLDHNASGKTEYMAFPKPFESSSSNGAEKQRRSHRQPEEEMEKRSAWLNDSQDVKKDDQSQLKASFAVSVQNIASNHKNQSDMSRRREFDEESLESFSSMPDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKNKRKSSSQLKGRITNIDYESASASSVCEPCKSNKSRHSEEVGHAKVFSKRNREQLEKIIKYSRSTEMSSETGSDLSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHLSENNEKGKCTKSLNSAAWIASNSELTPSESLASTDDETFGKNFSTEACQDCEQNDADNGSSMSTSSNFEPFATDDLGNTVIHLDQALARMREYERMKIEAESTLDSEGCSSNFQGASAAKLEGGQSASECVPVPQSSEASAVPCPHIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSISVKQRCKRKIEATEVIQSYAKEAKKGLQVDVCSSAEDVDEDKDKDETETVKQVQDSEMYDGNRVPESIRSDASDQEEDDESESGPVAISLSKAETQALTNYGSGEDENEDEEIEFEEGPVDVQTSLQASSETTTENEQNSNQEQSKTKSSEILSSEQESVYAKGEQHVATILPYYLNVMENAPPLTVNTPESFITDSMKTEESSSSLPVNETQTLDTTCVGNKSAASSESSMAGSPDTESPVLVNEYEAGSGNVSQRSDEDDFVKVEDLPLKLAVYSEADLMKKMAMEAQTNSLSDELLGGGGAQDQELVGDAQTLKEPETFGVQSA

Target ORF information:

RefSeq Version	XM_021285859.1
Organism	Columba livia(rock pigeon)
Definition	Columba livia pericentriolar material 1 (PCM1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021285859.1

ORF Insert Sequence:

ATGGCAACAG GAGGTGGTCC CTTTGAAGAA GGCATGAATG ATCAAGACTT GCCTAGCTGG 
AGCAATGACA GCTTTGATGA CCGGCTGAAC AACACGGACT GGGGAAGTCA ACAGAAGAAA 
GCAAACAGAT CTTCAGAAAA AAACAAGAAA AAGCTTGGTG GGGAAGCTGA AACAAGGCTT 
ACTAATGATA TATCTCCAGA ATCCTCACCT GGAATAGGAC GACGAAAGAC CAGAACTCCT 
CATAGTTTTC CTCATGCTCG ATACATGACC CAGATGTCTG TTCCGGAGCA GGCTGAGCTA 
GAAAGGCTTA AACAAAGAAT AAACTTCAGT GATCTGGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGCA GGGCAACAGC TGCTAACAAC AAACGTCAAC TTAATGAAAA CAAAAAACCA 
TTCAACTTCC TGTCACTGCA GATAAACACT AACAAGAGCA AAGATCCTGC CTCATGTTCC 
CAAAAAAAGG AAAGTGGGGT GTCAGTCCAA CGTAAAGAGT TGTTTGGTGC TGCTCTGAGC 
AAGGATTTCT TGCAAAATTG TCAAGTCTCT GCTCAAGAAG ATGGAGGGGG AGAACCAGCT 
ATGGATAGTA GCCAGATTGT GAGCAGACTA GTTCAAATTC GCGACTATAT TGCTAAGGCC 
AGCTCCATGC GGGATGATCT TGTAGAGAAA AATGAAAGAT CGGCCAATGT TGAGCGTTTA 
TCACACCTTA TAGATCACCT TAAAGAGCAG GAGAAATCCT ATCTGAAATT TTTGCAAAAG 
ATGCTTGCTA GACAAAATGA GGAGGATGAT GTTCGGACTA TAGATTCAGC TGTGGGATCT 
GGTTCTGTAG GTGAGAGCAC ATCGCTAAAC ATTGATGTGC AGTCTGAGGC TTCAGATACC 
ACGGAGGTAT CTTTTAGTTT GAGCATTCGG CCCTGCATTG AGGACAAACT AGGGAATTCA 
GCTTCACAAG AACAGGTTAC AGACTTTGAT GTTACTGCAA GCCCTAAAGG GAAAAATGAC 
AGAGCTGCCC TGAATGACAG GGAAATCTGG CCTTGTAGGA TTAATAGCCG AGAACATGGA 
TTGCCTTCAA AGGCTAGAGA TCCTCAACAG GAGGCTAAAG AGGAGTTGGA GAACTTGAAG 
AAGCAGCATG ATTTATTGAA AAGGATGCTA AAACAGCAAG AGCAATTAAA GGCTCTTCAA 
GGAAGACAGG CAGCTCTTCT TGCTTTGCAG CATAAAGCAG AGCAAGCAAT TGCTGTCATG 
GATGATTCTG TTGTAACAGA GACTACAGGT AGTGTTTCAG GAGTAAGCCT TACATCAGAA 
CTGAATGAAG AATTGAATGA CTTAATTCAG CGCTTTCACA ACCAACTTCA TGATTCTCAG 
ACACAGTCTG TACCAGACAA TAGAAGGCAA GCAGAAAGTC TTTCACTTAC TAGAGAGGTT 
TCACAAAGCA GAAACTCTTC AATGTCTGAA CGCCTGTCAG ATGAGAAGGC GCAGCTTTTT 
AACAAGATGC GAATGTTGCA GGGTAAAAAG CAAAAAATGG ACAAACTGTT AGGAGAACTT 
CATACACTTC GTGACCAACA TCTAAATAAC TCCTCCTTTT TTCCTGCTTC AGGTTCTCCT 
CAAAGAAGTA TTGATCAAAG AAGTACAACT TCAGCTGCTT CTGGTCCTGC AGGCATAGTA 
ACTGTTGTCA ATGGCGAATC AAATAGCCTG GCATCTGCTC CCTATCCTCC TGATTCCCTG 
GTTTCTCAGA ATGAGAGTGA AGAAGATGAC AATCTAAATC CAACAGAAAA GCTTCAGAAA 
CTAAATGAAG TTCGTAAGAG GCTGAATGAG TTACGTGAGC TAGTTCACTA CTATGAGCAA 
ACATCTGATA TGATGACAGA TTCTGTGAAT GAAAACACTA AGGAAGAAGA AGAAACCGAA 
GAATCAGAAA GTGATTCTGA ACGTGAGGAT CCACAGCCTG TTACAAATAT TAGAAATCCT 
CAAGGAATCA GTAGTTGGAG TGACATAAAT AGCAACTCAA ATGTACAGTG TGGAACTAAT 
AACAGAGATG GAAGACATCT TAATACAGAC TGTGAAATAA ATAACAGATC TGCTGCTAAC 
ATAAGGACTC TAAAAATGTC GTCACCTTTA GACTGTCATA ATAGGGAGGA TGACAAAGAT 
ATTGGCCTAC CCCAATGTGA AGATGAAGAA GTGGAAGAAG ATAGAGTTAG TGAAGATTCT 
GCATCTAGTC ACAGAAGCAG CCTGGGTGAT ACTGCTGGAG ATGCTGAGTT TGAGCAGAAG 
ATCAATAGGC TTATAGCTGC AAAACAGAAA CTTAGACAAT TACAAAACCT TGCTGCTATG 
GTGCAGGATG ATGATCCAGC ACCTCAGACA ACAATTGCAA ATGCATCTAA TATTGGTGAC 
TTGTTTTTGG GTGAGATGGA AGAGACAAAG CAGCAGCCAA ACAATGTCCG AGCTAGTACC 
AACAAATTAC AAAAAGATGT AGAACTGAAT GAAAAAGCAA GAGAGAAGTT TTATGAAGCT 
AAACTCCAGC AGCAGCAACA GGAGCTTAAG CAGTTACAAG AAGAAAGAAG AAAACTGATT 
GAAATTCAAG AAAAAATTCA GGTGTTACAG AAAGCTTGTC CTGATCTTCA ATTGTCAGCT 
GGCTTGGGTG ACTGCACAGC AAATAGACAG ACTTCACCAG CAACATCAAC TCCAGCCATG 
AATGACACAG CTGGCAAGCC TTCACTTGAA TTTGATGAAT CTGTACCAGT AGGCAATGAG 
TTATGGTCTG AGATGAGAAG ACATGAGATT TTAAGAGAAG AATTGCGACA AAGAAGAAAG 
CAACTGGAAG CTCTAATGGC TGAGCATCAG AGGAGGAAAG AGCTCGCAGA AACAATATCT 
ACTGTTGCTG CGTCTGTTAA AAGTGAAGGA TCAGAAGCTC GGCGTACTCC ACAGCAGAGT 
AGGACTGAAA ATAGGACAAT GGCTACCTGG GGAGGTTCTA CGCAGTGTGC ACTAGATGAA 
GAGGATGGAG ATGAAGATGG TTATCTCTCT GATGGAGCTG GTCAGGCAGA AGAAGATGAT 
GATGATGCGT CAAGTGCGAA TGACAGCTTC TCTGTTTATC CCAATAACAA CGTACCAGAA 
AATGCATATT TTGTTAAAGA AAACAAGGAT AGGTGGAAAA ATTACCGTCC TCTTTCAGCA 
GATGGAAATT ATCGTCCATT GTCTAAGGCC AGACAACAGC AAAACATAAG TATGCGACGT 
CAGGAAAACC TTCGGTGGAT GTCTGAACTT TCATATGTGG AAGAAAAGGA ACGATGGCAA 
GAGCAGATCA ATCAGTTGAA GAAACAGCAT GAATTTAGTG TCAGCATTTG TCAAACTTTA 
ATGCAGGATC AGCAGACCCT CTCTTGTCTT CTACAGACTT TGCTTACAGG CCCTTACAGT 
ATGATGCCCA ATAATGTTGC ATCTTCACAA GTACATCTTA TTATGCATCA GCTAAACCAG 
TGTTACACTC AACTGACATG GCAGCAGAAT AACGTCCAGA GGTTGAAACA AATGTTAAAT 
GATCTTGTGC GTCAGCAAGA AGAACAACGG TATGAAGAGA AGTCATCAAG AAAGGATAGA 
GGCAGTGGTG TACCCCCACC TCCATCTCCT GTTTTCTGTC CGTTCAGCTT TCCTCCACAA 
CCTTTGAACC TGTTTAATGT ACCAGGATTT ACTAATTTTT CTTCCTTTGC TCCAGATATT 
AACTATAATC CAGTGTTTCC TTCTGGTTTT GGAGATTTTG CACACAATAT TTCTCCACAC 
AGCAGTGAGC AGCAGCAGCA ACATCCTCTA GATCATAATG CATCTGGGAA AACTGAGTAT 
ATGGCGTTCC CCAAACCTTT TGAAAGCAGT TCCTCTAATG GAGCGGAAAA ACAAAGGAGG 
AGTCACAGAC AACCTGAAGA GGAAATGGAA AAAAGATCAG CTTGGCTTAA TGATAGCCAG 
GACGTGAAAA AAGATGATCA GTCTCAGCTG AAAGCAAGTT TTGCTGTTTC AGTACAAAAC 
ATTGCTTCCA ATCATAAAAA TCAGTCTGAT ATGAGCAGAA GAAGAGAGTT TGATGAAGAG 
TCTTTGGAGA GTTTTAGTAG CATGCCTGAT CCAGTAGACC CCACTACTGT GACAAAGACA 
TTTAAATCTA GAAAAGCATC AGCACAAGCA AGTTTGGCAT CAAAAGATAA AACACCTAAA 
TCGAAGAATA AGAGGAAGAG TTCTTCTCAG CTAAAAGGCA GAATTACAAA TATTGATTAT 
GAAAGTGCCA GTGCTTCTAG TGTGTGTGAA CCCTGCAAGA GCAATAAAAG CAGACACTCT 
GAAGAGGTTG GTCATGCAAA GGTGTTCAGC AAAAGGAATC GGGAACAACT GGAAAAAATC 
ATTAAATACA GTAGATCTAC AGAAATGTCT TCAGAAACTG GTAGTGATCT TTCTATGTTT 
GAAGCTTTAC GAGACACAAT TTATTCTGAA GTGGCAACTC TTATTTCTCA AAATGAGTCT 
CGTCCCCACT TTCTTATTGA ACTTTTCCAT GAGCTTCAGC TGCTAAATAC AGATTACCTG 
AGGCAAAGAG CTCTATACGC TCTACAGGAT ATAGTGACCA GACATTTATC TGAGAACAAT 
GAAAAAGGGA AGTGCACAAA ATCACTGAAT TCTGCAGCAT GGATAGCATC AAATTCTGAA 
CTCACTCCCA GTGAAAGCCT TGCTTCTACA GATGATGAAA CTTTTGGCAA AAACTTTTCT 
ACAGAAGCAT GTCAAGATTG TGAACAAAAT GATGCAGACA ATGGGAGTAG CATGTCTACA 
TCTTCAAATT TTGAACCCTT TGCCACTGAT GACCTTGGCA ACACAGTGAT TCACTTAGAT 
CAAGCTTTGG CTAGGATGAG GGAGTATGAG CGTATGAAAA TTGAAGCTGA AAGTACCCTT 
GACTCTGAGG GCTGCTCTAG TAATTTTCAG GGTGCTTCTG CTGCTAAATT AGAAGGAGGT 
CAAAGTGCCA GTGAATGTGT TCCTGTGCCA CAGTCAAGTG AAGCTTCTGC TGTTCCATGT 
CCTCATATAG ACACTCAGCA GCTTGACCGG CAGATTAAAG CAATTATGAA AGAGGTCATT 
CCTTTTCTGA AGGAACACAT GGATGAAGTA TGCTCTTCGC AGTTACTGAC ATCAGTAAGA 
CGTATGGTCT TGACCCTTAC ACAACAAAAT GATGAAAGTA AAGAGTTTGT GAAGTTCTTT 
CATAAGCAGC TTGGCAGTAT ACTTCAGGAT TCACTGGCAA AATTTGCTGG TAGAAAACTG 
AAAGATTGTG GAGAGGATCT TCTAGTGGAG ATCTCTGAAG TGTTATTTAA TGAATTAGCC 
TTTTTTAAAC TCATGCAAGA CTTGGACAAC AACAGTATTT CTGTAAAGCA GAGATGTAAA 
AGAAAAATAG AAGCTACTGA AGTGATACAG TCGTATGCTA AAGAGGCAAA AAAAGGTCTC 
CAGGTGGATG TTTGTTCATC TGCTGAAGAT GTCGATGAGG ACAAAGACAA GGATGAGACT 
GAAACTGTTA AACAAGTACA GGACTCAGAA ATGTATGATG GGAATAGAGT TCCTGAAAGT 
ATTAGGTCTG ATGCATCTGA TCAGGAGGAA GATGATGAAA GTGAAAGCGG TCCAGTGGCA 
ATAAGTTTAT CAAAAGCAGA AACCCAAGCT CTGACTAACT ATGGTAGTGG AGAAGATGAG 
AATGAAGATG AAGAAATAGA ATTTGAGGAA GGACCTGTTG ATGTGCAGAC GTCATTACAA 
GCCAGCAGTG AAACAACAAC TGAAAATGAA CAGAATTCAA ACCAAGAACA AAGTAAGACA 
AAAAGCAGTG AGATTCTGTC ATCAGAACAA GAATCTGTTT ATGCTAAAGG TGAACAACAT 
GTGGCTACAA TTTTGCCTTA TTACCTCAAT GTAATGGAGA ATGCACCGCC TTTAACGGTC 
AATACCCCAG AATCTTTTAT AACAGACAGT ATGAAAACAG AAGAATCAAG TTCATCTTTA 
CCAGTAAATG AAACTCAAAC ACTGGATACA ACGTGTGTAG GAAACAAATC TGCTGCAAGT 
TCTGAAAGCT CCATGGCTGG CAGCCCTGAT ACAGAGTCAC CTGTGTTAGT GAATGAATAT 
GAAGCTGGTT CTGGAAATGT AAGTCAAAGA TCTGATGAAG ATGACTTTGT GAAAGTTGAA 
GACTTGCCCC TTAAACTTGC AGTGTATTCA GAGGCAGATT TAATGAAGAA AATGGCAATG 
GAGGCCCAAA CGAACAGTTT GTCTGATGAA TTACTGGGTG GAGGTGGAGC TCAAGATCAA 
GAATTAGTAG GAGATGCCCA AACTTTGAAA GAACCTGAAA CTTTTGGAGT TCAAAGTGCG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_021285860.1 ) cDNA ORF clone, Columba livia(rock pigeon) -> XP_021141535.1 Columba livia pericentriolar material 1 (PCM1), transcript variant X7, mRNA.

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CloneID	OCo293163
Clone ID Related Accession (Same CDS sequence)	XM_021285860.1
Accession Version	XM_021285860.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-23
Organism	Columba livia(rock pigeon)
Product	pericentriolar material 1 protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004973253.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Columba livia Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACAG GAGGTGGTCC CTTTGAAGAA GGCATGAATG ATCAAGACTT GCCTAGCTGG 
AGCAATGACA GCTTTGATGA CCGGCTGAAC AACACGGACT GGGGAAGTCA ACAGAAGAAA 
GCAAACAGAT CTTCAGAAAA AAACAAGAAA AAGCTTGGTG GGGAAGCTGA AACAAGGCTT 
ACTAATGATA TATCTCCAGA ATCCTCACCT GGAATAGGAC GACGAAAGAC CAGAACTCCT 
CATAGTTTTC CTCATGCTCG ATACATGACC CAGATGTCTG TTCCGGAGCA GGCTGAGCTA 
GAAAGGCTTA AACAAAGAAT AAACTTCAGT GATCTGGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGCA GGGCAACAGC TGCTAACAAC AAACGTCAAC TTAATGAAAA CAAAAAACCA 
TTCAACTTCC TGTCACTGCA GATAAACACT AACAAGAGCA AAGATCCTGC CTCATGTTCC 
CAAAAAAAGG AAAGTGGGGT GTCAGTCCAA CGTAAAGAGT TGTTTGGTGC TGCTCTGAGC 
AAGGATTTCT TGCAAAATTG TCAAGTCTCT GCTCAAGAAG ATGGAGGGGG AGAACCAGCT 
ATGGATAGTA GCCAGATTGT GAGCAGACTA GTTCAAATTC GCGACTATAT TGCTAAGGCC 
AGCTCCATGC GGGATGATCT TGTAGAGAAA AATGAAAGAT CGGCCAATGT TGAGCGTTTA 
TCACACCTTA TAGATCACCT TAAAGAGCAG GAGAAATCCT ATCTGAAATT TTTGCAAAAG 
ATGCTTGCTA GACAAAATGA GGAGGATGAT GTTCGGACTA TAGATTCAGC TGTGGGATCT 
GGTTCTGTAG GTGAGAGCAC ATCGCTAAAC ATTGATGTGC AGTCTGAGGC TTCAGATACC 
ACGGAGGTAT CTTTTAGTTT GAGCATTCGG CCCTGCATTG AGGACAAACT AGGGAATTCA 
GCTTCACAAG AACAGGTTAC AGACTTTGAT GTTACTGCAA GCCCTAAAGG GAAAAATGAC 
AGAGCTGCCC TGAATGACAG GGAAATCTGG CCTTGTAGGA TTAATAGCCG AGAACATGGA 
TTGCCTTCAA AGGCTAGAGA TCCTCAACAG GAGGCTAAAG AGGAGTTGGA GAACTTGAAG 
AAGCAGCATG ATTTATTGAA AAGGATGCTA AAACAGCAAG AGCAATTAAA GGCTCTTCAA 
GGAAGACAGG CAGCTCTTCT TGCTTTGCAG CATAAAGCAG AGCAAGCAAT TGCTGTCATG 
GATGATTCTG TTGTAACAGA GACTACAGGT AGTGTTTCAG GAGTAAGCCT TACATCAGAA 
CTGAATGAAG AATTGAATGA CTTAATTCAG CGCTTTCACA ACCAACTTCA TGATTCTCAG 
ACACAGTCTG TACCAGACAA TAGAAGGCAA GCAGAAAGTC TTTCACTTAC TAGAGAGGTT 
TCACAAAGCA GAAACTCTTC AATGTCTGAA CGCCTGTCAG ATGAGAAGGC GCAGCTTTTT 
AACAAGATGC GAATGTTGCA GGGTAAAAAG CAAAAAATGG ACAAACTGTT AGGAGAACTT 
CATACACTTC GTGACCAACA TCTAAATAAC TCCTCCTTTT TTCCTGCTTC AGGTTCTCCT 
CAAAGAAGTA TTGATCAAAG AAGTACAACT TCAGCTGCTT CTGGTCCTGC AGGCATAGTA 
ACTGTTGTCA ATGGCGAATC AAATAGCCTG GCATCTGCTC CCTATCCTCC TGATTCCCTG 
GTTTCTCAGA ATGAGAGTGA AGAAGATGAC AATCTAAATC CAACAGAAAA GCTTCAGAAA 
CTAAATGAAG TTCGTAAGAG GCTGAATGAG TTACGTGAGC TAGTTCACTA CTATGAGCAA 
ACATCTGATA TGATGACAGA TTCTGTGAAT GAAAACACTA AGGAAGAAGA AGAAACCGAA 
GAATCAGAAA GTGATTCTGA ACGTGAGGAT CCACAGCCTG TTACAAATAT TAGAAATCCT 
CAAGGAATCA GTAGTTGGAG TGACATAAAT AGCAACTCAA ATGTACAGTG TGGAACTAAT 
AACAGAGATG GAAGACATCT TAATACAGAC TGTGAAATAA ATAACAGATC TGCTGCTAAC 
ATAAGGACTC TAAAAATGTC GTCACCTTTA GACTGTCATA ATAGGGAGGA TGACAAAGAT 
ATTGGCCTAC CCCAATGTGA AGATGAAGAA GTGGAAGAAG ATAGAGTTAG TGAAGATTCT 
GCATCTAGTC ACAGAAGCAG CCTGGGTGAT ACTGCTGGAG ATGCTGAGTT TGAGCAGAAG 
ATCAATAGGC TTATAGCTGC AAAACAGAAA CTTAGACAAT TACAAAACCT TGCTGCTATG 
GTGCAGGATG ATGATCCAGC ACCTCAGACA ACAATTGCAA ATGCATCTAA TATTGGTGAC 
TTGTTTTTGG GTGAGATGGA AGAGACAAAG CAGCAGCCAA ACAATGTCCG AGCTAGTACC 
AACAAATTAC AAAAAGATGT AGAACTGAAT GAAAAAGCAA GAGAGAAGTT TTATGAAGCT 
AAACTCCAGC AGCAGCAACA GGAGCTTAAG CAGTTACAAG AAGAAAGAAG AAAACTGATT 
GAAATTCAAG AAAAAATTCA GGTGTTACAG AAAGCTTGTC CTGATCTTCA ATTGTCAGCT 
GGCTTGGGTG ACTGCACAGC AAATAGACAG ACTTCACCAG CAACATCAAC TCCAGCCATG 
AATGACACAG CTGGCAAGCC TTCACTTGAA TTTGATGAAT CTGTACCAGT AGGCAATGAG 
TTATGGTCTG AGATGAGAAG ACATGAGATT TTAAGAGAAG AATTGCGACA AAGAAGAAAG 
CAACTGGAAG CTCTAATGGC TGAGCATCAG AGGAGGAAAG AGCTCGCAGA AACAATATCT 
ACTGTTGCTG CGTCTGTTAA AAGTGAAGGA TCAGAAGCTC GGCGTACTCC ACAGCAGAGT 
AGGACTGAAA ATAGGACAAT GGCTACCTGG GGAGGTTCTA CGCAGTGTGC ACTAGATGAA 
GAGGATGGAG ATGAAGATGG TTATCTCTCT GATGGAGCTG GTCAGGCAGA AGAAGATGAT 
GATGATGCGT CAAGTGCGAA TGACAGCTTC TCTGTTTATC CCAATAACAA CGTACCAGAA 
AATGCATATT TTGTTAAAGA AAACAAGGAT AGGTGGAAAA ATTACCGTCC TCTTTCAGCA 
GATGGAAATT ATCGTCCATT GTCTAAGGCC AGACAACAGC AAAACATAAG TATGCGACGT 
CAGGAAAACC TTCGGTGGAT GTCTGAACTT TCATATGTGG AAGAAAAGGA ACGATGGCAA 
GAGCAGATCA ATCAGTTGAA GAAACAGCAT GAATTTAGTG TCAGCATTTG TCAAACTTTA 
ATGCAGGATC AGCAGACCCT CTCTTGTCTT CTACAGACTT TGCTTACAGG CCCTTACAGT 
ATGATGCCCA ATAATGTTGC ATCTTCACAA GTACATCTTA TTATGCATCA GCTAAACCAG 
TGTTACACTC AACTGACATG GCAGCAGAAT AACGTCCAGA GGTTGAAACA AATGTTAAAT 
GATCTTGTGC GTCAGCAAGA AGAACAACGG TATGAAGAGA AGTCATCAAG AAAGGATAGA 
GGCAGTGGTG TACCCCCACC TCCATCTCCT GTTTTCTGTC CGTTCAGCTT TCCTCCACAA 
CCTTTGAACC TGTTTAATGT ACCAGGATTT ACTAATTTTT CTTCCTTTGC TCCAGATATT 
AACTATAATC CAGTGTTTCC TTCTGGTTTT GGAGATTTTG CACACAATAT TTCTCCACAC 
AGCAGTGAGC AGCAGCAGCA ACATCCTCTA GATCATAATG CATCTGGGAA AACTGAGTAT 
ATGGCGTTCC CCAAACCTTT TGAAAGCAGT TCCTCTAATG GAGCGGAAAA ACAAAGGAGG 
AGTCACAGAC AACCTGAAGA GGAAATGGAA AAAAGATCAG CTTGGCTTAA TGATAGCCAG 
GACGTGAAAA AAGATGATCA GTCTCAGCTG AAAGCAAGTT TTGCTGTTTC AGTACAAAAC 
ATTGCTTCCA ATCATAAAAA TCAGTCTGAT ATGAGCAGAA GAAGAGAGTT TGATGAAGAG 
TCTTTGGAGA GTTTTAGTAG CATGCCTGAT CCAGTAGACC CCACTACTGT GACAAAGACA 
TTTAAATCTA GAAAAGCATC AGCACAAGCA AGTTTGGCAT CAAAAGATAA AACACCTAAA 
TCGAAGAATA AGAGGAAGAG TTCTTCTCAG CTAAAAGGCA GAATTACAAA TATTGATTAT 
GAAAGTGCCA GTGCTTCTAG TGTGTGTGAA CCCTGCAAGA GCAATAAAAG CAGACACTCT 
GAAGAGGTTG GTCATGCAAA GGTGTTCAGC AAAAGGAATC GGGAACAACT GGAAAAAATC 
ATTAAATACA GTAGATCTAC AGAAATGTCT TCAGCGCATG CTAGGAGAAT TCTGCAGCAG 
TCTAACAGAA ATGCATGCAT TGAAGCGCCA GAAACTGGTA GTGATCTTTC TATGTTTGAA 
GCTTTACGAG ACACAATTTA TTCTGAAGTG GCAACTCTTA TTTCTCAAAA TGAGTCTCGT 
CCCCACTTTC TTATTGAACT TTTCCATGAG CTTCAGCTGC TAAATACAGA TTACCTGAGG 
CAAAGAGCTC TATACGCTCT ACAGGATATA GTGACCAGAC ATTTATCTGA GAACAATGAA 
AAAGGGAAGT GCACAAAATC ACTGAATTCT GCAGCATGGA TAGCATCAAA TTCTGAACTC 
ACTCCCAGTG AAAGCCTTGC TTCTACAGAT GATGAAACTT TTGGCAAAAA CTTTTCTACA 
GAAGCATGTC AAGATTGTGA ACAAAATGAT GCAGACAATG GGAGTAGCAT GTCTACATCT 
TCAAATTTTG AACCCTTTGC CACTGATGAC CTTGGCAACA CAGTGATTCA CTTAGATCAA 
GCTTTGGCTA GGATGAGGGA GTATGAGCGT ATGAAAATTG AAGCTGAAAG TACCCTTGAC 
TCTGAGGGCT GCTCTAGTAA TTTTCAGGGT GCTTCTGCTG CTAAATTAGA AGGAGGTCAA 
AGTGCCAGTG AATGTGTTCC TGTGCCACAG TCAAGTGAAG CTTCTGCTGT TCCATGTCCT 
CATATAGACA CTCAGCAGCT TGACCGGCAG ATTAAAGCAA TTATGAAAGA GGTCATTCCT 
TTTCTGAAGG AACACATGGA TGAAGTATGC TCTTCGCAGT TACTGACATC AGTAAGACGT 
ATGGTCTTGA CCCTTACACA ACAAAATGAT GAAAGTAAAG AGTTTGTGAA GTTCTTTCAT 
AAGCAGCTTG GCAGTATACT TCAGGATTCA CTGGCAAAAT TTGCTGGTAG AAAACTGAAA 
GATTGTGGAG AGGATCTTCT AGTGGAGATC TCTGAAGTGT TATTTAATGA ATTAGCCTTT 
TTTAAACTCA TGCAAGACTT GGACAACAAC AGTATTTCTG TAAAGCAGAG ATGTAAAAGA 
AAAATAGAAG CTACTGAAGT GATACAGTCG TATGCTAAAG AGGACAAGGA TGAGACTGAA 
ACTGTTAAAC AAGTACAGGA CTCAGAAATG TATGATGGGA ATAGAGTTCC TGAAAGTATT 
AGGTCTGATG CATCTGATCA GGAGGAAGAT GATGAAAGTG AAAGCGGTCC AGTGGCAATA 
AGTTTATCAA AAGCAGAAAC CCAAGCTCTG ACTAACTATG GTAGTGGAGA AGATGAGAAT 
GAAGATGAAG AAATAGAATT TGAGGAAGGA CCTGTTGATG TGCAGACGTC ATTACAAGCC 
AGCAGTGAAA CAACAACTGA AAATGAACAG AATTCAAACC AAGAACAAAG TAAGACAAAA 
AGCAGTGAGA TTCTGTCATC AGAACAAGAA TCTGTTTATG CTAAAGGTGA ACAACATGTG 
GCTACAATTT TGCCTTATTA CCTCAATGTA ATGGAGAATG CACCGCCTTT AACGGTCAAT 
ACCCCAGAAT CTTTTATAAC AGACAGTATG AAAACAGAAG AATCAAGTTC ATCTTTACCA 
GTAAATGAAA CTCAAACACT GGATACAACG TGTGTAGGAA ACAAATCTGC TGCAAGTTCT 
GAAAGCTCCA TGGCTGGCAG CCCTGATACA GAGTCACCTG TGTTAGTGAA TGAATATGAA 
GCTGGTTCTG GAAATGTAAG TCAAAGATCT GATGAAGATG ACTTTGTGAA AGTTGAAGAC 
TTGCCCCTTA AACTTGCAGT GTATTCAGAG GCAGATTTAA TGAAGAAAAT GGCAATGGAG 
GCCCAAACGA ACAGTTTGTC TGATGAATTA CTGGGTGGAG GTGGAGCTCA AGATCAAGAA 
TTAGTAGGAG ATGCCCAAAC TTTGAAAGAA CCTGAAACTT TTGGAGTTCA AAGTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021141535.1
CDS	140..6499
Translation	MATGGGPFEEGMNDQDLPSWSNDSFDDRLNNTDWGSQQKKANRSSEKNKKKLGGEAETRLTNDISPESSPGIGRRKTRTPHSFPHARYMTQMSVPEQAELERLKQRINFSDLDQRSIGSDSQGRATAANNKRQLNENKKPFNFLSLQINTNKSKDPASCSQKKESGVSVQRKELFGAALSKDFLQNCQVSAQEDGGGEPAMDSSQIVSRLVQIRDYIAKASSMRDDLVEKNERSANVERLSHLIDHLKEQEKSYLKFLQKMLARQNEEDDVRTIDSAVGSGSVGESTSLNIDVQSEASDTTEVSFSLSIRPCIEDKLGNSASQEQVTDFDVTASPKGKNDRAALNDREIWPCRINSREHGLPSKARDPQQEAKEELENLKKQHDLLKRMLKQQEQLKALQGRQAALLALQHKAEQAIAVMDDSVVTETTGSVSGVSLTSELNEELNDLIQRFHNQLHDSQTQSVPDNRRQAESLSLTREVSQSRNSSMSERLSDEKAQLFNKMRMLQGKKQKMDKLLGELHTLRDQHLNNSSFFPASGSPQRSIDQRSTTSAASGPAGIVTVVNGESNSLASAPYPPDSLVSQNESEEDDNLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDSVNENTKEEEETEESESDSEREDPQPVTNIRNPQGISSWSDINSNSNVQCGTNNRDGRHLNTDCEINNRSAANIRTLKMSSPLDCHNREDDKDIGLPQCEDEEVEEDRVSEDSASSHRSSLGDTAGDAEFEQKINRLIAAKQKLRQLQNLAAMVQDDDPAPQTTIANASNIGDLFLGEMEETKQQPNNVRASTNKLQKDVELNEKAREKFYEAKLQQQQQELKQLQEERRKLIEIQEKIQVLQKACPDLQLSAGLGDCTANRQTSPATSTPAMNDTAGKPSLEFDESVPVGNELWSEMRRHEILREELRQRRKQLEALMAEHQRRKELAETISTVAASVKSEGSEARRTPQQSRTENRTMATWGGSTQCALDEEDGDEDGYLSDGAGQAEEDDDDASSANDSFSVYPNNNVPENAYFVKENKDRWKNYRPLSADGNYRPLSKARQQQNISMRRQENLRWMSELSYVEEKERWQEQINQLKKQHEFSVSICQTLMQDQQTLSCLLQTLLTGPYSMMPNNVASSQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNDLVRQQEEQRYEEKSSRKDRGSGVPPPPSPVFCPFSFPPQPLNLFNVPGFTNFSSFAPDINYNPVFPSGFGDFAHNISPHSSEQQQQHPLDHNASGKTEYMAFPKPFESSSSNGAEKQRRSHRQPEEEMEKRSAWLNDSQDVKKDDQSQLKASFAVSVQNIASNHKNQSDMSRRREFDEESLESFSSMPDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKNKRKSSSQLKGRITNIDYESASASSVCEPCKSNKSRHSEEVGHAKVFSKRNREQLEKIIKYSRSTEMSSAHARRILQQSNRNACIEAPETGSDLSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHLSENNEKGKCTKSLNSAAWIASNSELTPSESLASTDDETFGKNFSTEACQDCEQNDADNGSSMSTSSNFEPFATDDLGNTVIHLDQALARMREYERMKIEAESTLDSEGCSSNFQGASAAKLEGGQSASECVPVPQSSEASAVPCPHIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSISVKQRCKRKIEATEVIQSYAKEDKDETETVKQVQDSEMYDGNRVPESIRSDASDQEEDDESESGPVAISLSKAETQALTNYGSGEDENEDEEIEFEEGPVDVQTSLQASSETTTENEQNSNQEQSKTKSSEILSSEQESVYAKGEQHVATILPYYLNVMENAPPLTVNTPESFITDSMKTEESSSSLPVNETQTLDTTCVGNKSAASSESSMAGSPDTESPVLVNEYEAGSGNVSQRSDEDDFVKVEDLPLKLAVYSEADLMKKMAMEAQTNSLSDELLGGGGAQDQELVGDAQTLKEPETFGVQSA

Target ORF information:

RefSeq Version	XM_021285860.1
Organism	Columba livia(rock pigeon)
Definition	Columba livia pericentriolar material 1 (PCM1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021285860.1

ORF Insert Sequence:

ATGGCAACAG GAGGTGGTCC CTTTGAAGAA GGCATGAATG ATCAAGACTT GCCTAGCTGG 
AGCAATGACA GCTTTGATGA CCGGCTGAAC AACACGGACT GGGGAAGTCA ACAGAAGAAA 
GCAAACAGAT CTTCAGAAAA AAACAAGAAA AAGCTTGGTG GGGAAGCTGA AACAAGGCTT 
ACTAATGATA TATCTCCAGA ATCCTCACCT GGAATAGGAC GACGAAAGAC CAGAACTCCT 
CATAGTTTTC CTCATGCTCG ATACATGACC CAGATGTCTG TTCCGGAGCA GGCTGAGCTA 
GAAAGGCTTA AACAAAGAAT AAACTTCAGT GATCTGGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGCA GGGCAACAGC TGCTAACAAC AAACGTCAAC TTAATGAAAA CAAAAAACCA 
TTCAACTTCC TGTCACTGCA GATAAACACT AACAAGAGCA AAGATCCTGC CTCATGTTCC 
CAAAAAAAGG AAAGTGGGGT GTCAGTCCAA CGTAAAGAGT TGTTTGGTGC TGCTCTGAGC 
AAGGATTTCT TGCAAAATTG TCAAGTCTCT GCTCAAGAAG ATGGAGGGGG AGAACCAGCT 
ATGGATAGTA GCCAGATTGT GAGCAGACTA GTTCAAATTC GCGACTATAT TGCTAAGGCC 
AGCTCCATGC GGGATGATCT TGTAGAGAAA AATGAAAGAT CGGCCAATGT TGAGCGTTTA 
TCACACCTTA TAGATCACCT TAAAGAGCAG GAGAAATCCT ATCTGAAATT TTTGCAAAAG 
ATGCTTGCTA GACAAAATGA GGAGGATGAT GTTCGGACTA TAGATTCAGC TGTGGGATCT 
GGTTCTGTAG GTGAGAGCAC ATCGCTAAAC ATTGATGTGC AGTCTGAGGC TTCAGATACC 
ACGGAGGTAT CTTTTAGTTT GAGCATTCGG CCCTGCATTG AGGACAAACT AGGGAATTCA 
GCTTCACAAG AACAGGTTAC AGACTTTGAT GTTACTGCAA GCCCTAAAGG GAAAAATGAC 
AGAGCTGCCC TGAATGACAG GGAAATCTGG CCTTGTAGGA TTAATAGCCG AGAACATGGA 
TTGCCTTCAA AGGCTAGAGA TCCTCAACAG GAGGCTAAAG AGGAGTTGGA GAACTTGAAG 
AAGCAGCATG ATTTATTGAA AAGGATGCTA AAACAGCAAG AGCAATTAAA GGCTCTTCAA 
GGAAGACAGG CAGCTCTTCT TGCTTTGCAG CATAAAGCAG AGCAAGCAAT TGCTGTCATG 
GATGATTCTG TTGTAACAGA GACTACAGGT AGTGTTTCAG GAGTAAGCCT TACATCAGAA 
CTGAATGAAG AATTGAATGA CTTAATTCAG CGCTTTCACA ACCAACTTCA TGATTCTCAG 
ACACAGTCTG TACCAGACAA TAGAAGGCAA GCAGAAAGTC TTTCACTTAC TAGAGAGGTT 
TCACAAAGCA GAAACTCTTC AATGTCTGAA CGCCTGTCAG ATGAGAAGGC GCAGCTTTTT 
AACAAGATGC GAATGTTGCA GGGTAAAAAG CAAAAAATGG ACAAACTGTT AGGAGAACTT 
CATACACTTC GTGACCAACA TCTAAATAAC TCCTCCTTTT TTCCTGCTTC AGGTTCTCCT 
CAAAGAAGTA TTGATCAAAG AAGTACAACT TCAGCTGCTT CTGGTCCTGC AGGCATAGTA 
ACTGTTGTCA ATGGCGAATC AAATAGCCTG GCATCTGCTC CCTATCCTCC TGATTCCCTG 
GTTTCTCAGA ATGAGAGTGA AGAAGATGAC AATCTAAATC CAACAGAAAA GCTTCAGAAA 
CTAAATGAAG TTCGTAAGAG GCTGAATGAG TTACGTGAGC TAGTTCACTA CTATGAGCAA 
ACATCTGATA TGATGACAGA TTCTGTGAAT GAAAACACTA AGGAAGAAGA AGAAACCGAA 
GAATCAGAAA GTGATTCTGA ACGTGAGGAT CCACAGCCTG TTACAAATAT TAGAAATCCT 
CAAGGAATCA GTAGTTGGAG TGACATAAAT AGCAACTCAA ATGTACAGTG TGGAACTAAT 
AACAGAGATG GAAGACATCT TAATACAGAC TGTGAAATAA ATAACAGATC TGCTGCTAAC 
ATAAGGACTC TAAAAATGTC GTCACCTTTA GACTGTCATA ATAGGGAGGA TGACAAAGAT 
ATTGGCCTAC CCCAATGTGA AGATGAAGAA GTGGAAGAAG ATAGAGTTAG TGAAGATTCT 
GCATCTAGTC ACAGAAGCAG CCTGGGTGAT ACTGCTGGAG ATGCTGAGTT TGAGCAGAAG 
ATCAATAGGC TTATAGCTGC AAAACAGAAA CTTAGACAAT TACAAAACCT TGCTGCTATG 
GTGCAGGATG ATGATCCAGC ACCTCAGACA ACAATTGCAA ATGCATCTAA TATTGGTGAC 
TTGTTTTTGG GTGAGATGGA AGAGACAAAG CAGCAGCCAA ACAATGTCCG AGCTAGTACC 
AACAAATTAC AAAAAGATGT AGAACTGAAT GAAAAAGCAA GAGAGAAGTT TTATGAAGCT 
AAACTCCAGC AGCAGCAACA GGAGCTTAAG CAGTTACAAG AAGAAAGAAG AAAACTGATT 
GAAATTCAAG AAAAAATTCA GGTGTTACAG AAAGCTTGTC CTGATCTTCA ATTGTCAGCT 
GGCTTGGGTG ACTGCACAGC AAATAGACAG ACTTCACCAG CAACATCAAC TCCAGCCATG 
AATGACACAG CTGGCAAGCC TTCACTTGAA TTTGATGAAT CTGTACCAGT AGGCAATGAG 
TTATGGTCTG AGATGAGAAG ACATGAGATT TTAAGAGAAG AATTGCGACA AAGAAGAAAG 
CAACTGGAAG CTCTAATGGC TGAGCATCAG AGGAGGAAAG AGCTCGCAGA AACAATATCT 
ACTGTTGCTG CGTCTGTTAA AAGTGAAGGA TCAGAAGCTC GGCGTACTCC ACAGCAGAGT 
AGGACTGAAA ATAGGACAAT GGCTACCTGG GGAGGTTCTA CGCAGTGTGC ACTAGATGAA 
GAGGATGGAG ATGAAGATGG TTATCTCTCT GATGGAGCTG GTCAGGCAGA AGAAGATGAT 
GATGATGCGT CAAGTGCGAA TGACAGCTTC TCTGTTTATC CCAATAACAA CGTACCAGAA 
AATGCATATT TTGTTAAAGA AAACAAGGAT AGGTGGAAAA ATTACCGTCC TCTTTCAGCA 
GATGGAAATT ATCGTCCATT GTCTAAGGCC AGACAACAGC AAAACATAAG TATGCGACGT 
CAGGAAAACC TTCGGTGGAT GTCTGAACTT TCATATGTGG AAGAAAAGGA ACGATGGCAA 
GAGCAGATCA ATCAGTTGAA GAAACAGCAT GAATTTAGTG TCAGCATTTG TCAAACTTTA 
ATGCAGGATC AGCAGACCCT CTCTTGTCTT CTACAGACTT TGCTTACAGG CCCTTACAGT 
ATGATGCCCA ATAATGTTGC ATCTTCACAA GTACATCTTA TTATGCATCA GCTAAACCAG 
TGTTACACTC AACTGACATG GCAGCAGAAT AACGTCCAGA GGTTGAAACA AATGTTAAAT 
GATCTTGTGC GTCAGCAAGA AGAACAACGG TATGAAGAGA AGTCATCAAG AAAGGATAGA 
GGCAGTGGTG TACCCCCACC TCCATCTCCT GTTTTCTGTC CGTTCAGCTT TCCTCCACAA 
CCTTTGAACC TGTTTAATGT ACCAGGATTT ACTAATTTTT CTTCCTTTGC TCCAGATATT 
AACTATAATC CAGTGTTTCC TTCTGGTTTT GGAGATTTTG CACACAATAT TTCTCCACAC 
AGCAGTGAGC AGCAGCAGCA ACATCCTCTA GATCATAATG CATCTGGGAA AACTGAGTAT 
ATGGCGTTCC CCAAACCTTT TGAAAGCAGT TCCTCTAATG GAGCGGAAAA ACAAAGGAGG 
AGTCACAGAC AACCTGAAGA GGAAATGGAA AAAAGATCAG CTTGGCTTAA TGATAGCCAG 
GACGTGAAAA AAGATGATCA GTCTCAGCTG AAAGCAAGTT TTGCTGTTTC AGTACAAAAC 
ATTGCTTCCA ATCATAAAAA TCAGTCTGAT ATGAGCAGAA GAAGAGAGTT TGATGAAGAG 
TCTTTGGAGA GTTTTAGTAG CATGCCTGAT CCAGTAGACC CCACTACTGT GACAAAGACA 
TTTAAATCTA GAAAAGCATC AGCACAAGCA AGTTTGGCAT CAAAAGATAA AACACCTAAA 
TCGAAGAATA AGAGGAAGAG TTCTTCTCAG CTAAAAGGCA GAATTACAAA TATTGATTAT 
GAAAGTGCCA GTGCTTCTAG TGTGTGTGAA CCCTGCAAGA GCAATAAAAG CAGACACTCT 
GAAGAGGTTG GTCATGCAAA GGTGTTCAGC AAAAGGAATC GGGAACAACT GGAAAAAATC 
ATTAAATACA GTAGATCTAC AGAAATGTCT TCAGCGCATG CTAGGAGAAT TCTGCAGCAG 
TCTAACAGAA ATGCATGCAT TGAAGCGCCA GAAACTGGTA GTGATCTTTC TATGTTTGAA 
GCTTTACGAG ACACAATTTA TTCTGAAGTG GCAACTCTTA TTTCTCAAAA TGAGTCTCGT 
CCCCACTTTC TTATTGAACT TTTCCATGAG CTTCAGCTGC TAAATACAGA TTACCTGAGG 
CAAAGAGCTC TATACGCTCT ACAGGATATA GTGACCAGAC ATTTATCTGA GAACAATGAA 
AAAGGGAAGT GCACAAAATC ACTGAATTCT GCAGCATGGA TAGCATCAAA TTCTGAACTC 
ACTCCCAGTG AAAGCCTTGC TTCTACAGAT GATGAAACTT TTGGCAAAAA CTTTTCTACA 
GAAGCATGTC AAGATTGTGA ACAAAATGAT GCAGACAATG GGAGTAGCAT GTCTACATCT 
TCAAATTTTG AACCCTTTGC CACTGATGAC CTTGGCAACA CAGTGATTCA CTTAGATCAA 
GCTTTGGCTA GGATGAGGGA GTATGAGCGT ATGAAAATTG AAGCTGAAAG TACCCTTGAC 
TCTGAGGGCT GCTCTAGTAA TTTTCAGGGT GCTTCTGCTG CTAAATTAGA AGGAGGTCAA 
AGTGCCAGTG AATGTGTTCC TGTGCCACAG TCAAGTGAAG CTTCTGCTGT TCCATGTCCT 
CATATAGACA CTCAGCAGCT TGACCGGCAG ATTAAAGCAA TTATGAAAGA GGTCATTCCT 
TTTCTGAAGG AACACATGGA TGAAGTATGC TCTTCGCAGT TACTGACATC AGTAAGACGT 
ATGGTCTTGA CCCTTACACA ACAAAATGAT GAAAGTAAAG AGTTTGTGAA GTTCTTTCAT 
AAGCAGCTTG GCAGTATACT TCAGGATTCA CTGGCAAAAT TTGCTGGTAG AAAACTGAAA 
GATTGTGGAG AGGATCTTCT AGTGGAGATC TCTGAAGTGT TATTTAATGA ATTAGCCTTT 
TTTAAACTCA TGCAAGACTT GGACAACAAC AGTATTTCTG TAAAGCAGAG ATGTAAAAGA 
AAAATAGAAG CTACTGAAGT GATACAGTCG TATGCTAAAG AGGACAAGGA TGAGACTGAA 
ACTGTTAAAC AAGTACAGGA CTCAGAAATG TATGATGGGA ATAGAGTTCC TGAAAGTATT 
AGGTCTGATG CATCTGATCA GGAGGAAGAT GATGAAAGTG AAAGCGGTCC AGTGGCAATA 
AGTTTATCAA AAGCAGAAAC CCAAGCTCTG ACTAACTATG GTAGTGGAGA AGATGAGAAT 
GAAGATGAAG AAATAGAATT TGAGGAAGGA CCTGTTGATG TGCAGACGTC ATTACAAGCC 
AGCAGTGAAA CAACAACTGA AAATGAACAG AATTCAAACC AAGAACAAAG TAAGACAAAA 
AGCAGTGAGA TTCTGTCATC AGAACAAGAA TCTGTTTATG CTAAAGGTGA ACAACATGTG 
GCTACAATTT TGCCTTATTA CCTCAATGTA ATGGAGAATG CACCGCCTTT AACGGTCAAT 
ACCCCAGAAT CTTTTATAAC AGACAGTATG AAAACAGAAG AATCAAGTTC ATCTTTACCA 
GTAAATGAAA CTCAAACACT GGATACAACG TGTGTAGGAA ACAAATCTGC TGCAAGTTCT 
GAAAGCTCCA TGGCTGGCAG CCCTGATACA GAGTCACCTG TGTTAGTGAA TGAATATGAA 
GCTGGTTCTG GAAATGTAAG TCAAAGATCT GATGAAGATG ACTTTGTGAA AGTTGAAGAC 
TTGCCCCTTA AACTTGCAGT GTATTCAGAG GCAGATTTAA TGAAGAAAAT GGCAATGGAG 
GCCCAAACGA ACAGTTTGTC TGATGAATTA CTGGGTGGAG GTGGAGCTCA AGATCAAGAA 
TTAGTAGGAG ATGCCCAAAC TTTGAAAGAA CCTGAAACTT TTGGAGTTCA AAGTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_021285861.1 ) cDNA ORF clone, Columba livia(rock pigeon) -> XP_021141536.1 Columba livia pericentriolar material 1 (PCM1), transcript variant X8, mRNA.

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CloneID	OCo293164
Clone ID Related Accession (Same CDS sequence)	XM_021285861.1
Accession Version	XM_021285861.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-23
Organism	Columba livia(rock pigeon)
Product	pericentriolar material 1 protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004973253.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Columba livia Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACAG GAGGTGGTCC CTTTGAAGAA GGCATGAATG ATCAAGACTT GCCTAGCTGG 
AGCAATGACA GCTTTGATGA CCGGCTGAAC AACACGGACT GGGGAAGTCA ACAGAAGAAA 
GCAAACAGAT CTTCAGAAAA AAACAAGAAA AAGCTTGGTG GGGAAGCTGA AACAAGGCTT 
ACTAATGATA TATCTCCAGA ATCCTCACCT GGAATAGGAC GACGAAAGAC CAGAACTCCT 
CATAGTTTTC CTCATGCTCG ATACATGACC CAGATGTCTG TTCCGGAGCA GGCTGAGCTA 
GAAAGGCTTA AACAAAGAAT AAACTTCAGT GATCTGGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGCA GGGCAACAGC TGCTAACAAC AAACGTCAAC TTAATGAAAA CAAAAAACCA 
TTCAACTTCC TGTCACTGCA GATAAACACT AACAAGAGCA AAGATCCTGC CTCATGTTCC 
CAAAAAAAGG AAAGTGGGGT GTCAGTCCAA CGTAAAGAGT TGTTTGGTGC TGCTCTGAGC 
AAGGATTTCT TGCAAAATTG TCAAGTCTCT GCTCAAGAAG ATGGAGGGGG AGAACCAGCT 
ATGGATAGTA GCCAGATTGT GAGCAGACTA GTTCAAATTC GCGACTATAT TGCTAAGGCC 
AGCTCCATGC GGGATGATCT TGTAGAGAAA AATGAAAGAT CGGCCAATGT TGAGCGTTTA 
TCACACCTTA TAGATCACCT TAAAGAGCAG GAGAAATCCT ATCTGAAATT TTTGCAAAAG 
ATGCTTGCTA GACAAAATGA GGAGGATGAT GTTCGGACTA TAGATTCAGC TGTGGGATCT 
GGTTCTGTAG GTGAGAGCAC ATCGCTAAAC ATTGATGTGC AGTCTGAGGC TTCAGATACC 
ACGGAGGTAT CTTTTAGTTT GAGCATTCGG CCCTGCATTG AGGACAAACT AGGGAATTCA 
GCTTCACAAG AACAGGTTAC AGACTTTGAT GTTACTGCAA GCCCTAAAGG GAAAAATGAC 
AGAGCTGCCC TGAATGACAG GGAAATCTGG CCTTGTAGGA TTAATAGCCG AGAACATGGA 
TTGCCTTCAA AGGCTAGAGA TCCTCAACAG GAGGCTAAAG AGGAGTTGGA GAACTTGAAG 
AAGCAGCATG ATTTATTGAA AAGGATGCTA AAACAGCAAG AGCAATTAAA GGCTCTTCAA 
GGAAGACAGG CAGCTCTTCT TGCTTTGCAG CATAAAGCAG AGCAAGCAAT TGCTGTCATG 
GATGATTCTG TTGTAACAGA GACTACAGGT AGTGTTTCAG GAGTAAGCCT TACATCAGAA 
CTGAATGAAG AATTGAATGA CTTAATTCAG CGCTTTCACA ACCAACTTCA TGATTCTCAG 
ACACAGTCTG TACCAGACAA TAGAAGGCAA GCAGAAAGTC TTTCACTTAC TAGAGAGGTT 
TCACAAAGCA GAAACTCTTC AATGTCTGAA CGCCTGTCAG ATGAGAAGGC GCAGCTTTTT 
AACAAGATGC GAATGTTGCA GGGTAAAAAG CAAAAAATGG ACAAACTGTT AGGAGAACTT 
CATACACTTC GTGACCAACA TCTAAATAAC TCCTCCTTTT TTCCTGCTTC AGGTTCTCCT 
CAAAGAAGTA TTGATCAAAG AAGTACAACT TCAGCTGCTT CTGGTCCTGC AGGCATAGTA 
ACTGTTGTCA ATGGCGAATC AAATAGCCTG GCATCTGCTC CCTATCCTCC TGATTCCCTG 
GTTTCTCAGA ATGAGAGTGA AGAAGATGAC AATCTAAATC CAACAGAAAA GCTTCAGAAA 
CTAAATGAAG TTCGTAAGAG GCTGAATGAG TTACGTGAGC TAGTTCACTA CTATGAGCAA 
ACATCTGATA TGATGACAGA TTCTGTGAAT GAAAACACTA AGGAAGAAGA AGAAACCGAA 
GAATCAGAAA GTGATTCTGA ACGTGAGGAT CCACAGCCTG TTACAAATAT TAGAAATCCT 
CAAGGAATCA GTAGTTGGAG TGACATAAAT AGCAACTCAA ATGTACAGTG TGGAACTAAT 
AACAGAGATG GAAGACATCT TAATACAGAC TGTGAAATAA ATAACAGATC TGCTGCTAAC 
ATAAGGACTC TAAAAATGTC GTCACCTTTA GACTGTCATA ATAGGGAGGA TGACAAAGAT 
ATTGGCCTAC CCCAATGTGA AGATGAAGAA GTGGAAGAAG ATAGAGTTAG TGAAGATTCT 
GCATCTAGTC ACAGAAGCAG CCTGGGTGAT ACTGCTGGAG ATGCTGAGTT TGAGCAGAAG 
ATCAATAGGC TTATAGCTGC AAAACAGAAA CTTAGACAAT TACAAAACCT TGCTGCTATG 
GTGCAGGATG ATGATCCAGC ACCTCAGACA ACAATTGCAA ATGCATCTAA TATTGGTGAC 
TTGTTTTTGG GTGAGATGGA AGAGACAAAG CAGCAGCCAA ACAATGTCCG AGCTAGTACC 
AACAAATTAC AAAAAGATGT AGAACTGAAT GAAAAAGCAA GAGAGAAGTT TTATGAAGCT 
AAACTCCAGC AGCAGCAACA GGAGCTTAAG CAGTTACAAG AAGAAAGAAG AAAACTGATT 
GAAATTCAAG AAAAAATTCA GGTGTTACAG AAAGCTTGTC CTGATCTTCA ATTGTCAGCT 
GGCTTGGGTG ACTGCACAGC AAATAGACAG ACTTCACCAG CAACATCAAC TCCAGCCATG 
AATGACACAG CTGGCAAGCC TTCACTTGAA TTTGATGAAT CTGTACCAGT AGGCAATGAG 
TTATGGTCTG AGATGAGAAG ACATGAGATT TTAAGAGAAG AATTGCGACA AAGAAGAAAG 
CAACTGGAAG CTCTAATGGC TGAGCATCAG AGGAGGAAAG AGCTCGCAGA AACAATATCT 
ACTGTTGCTG CGTCTGTTAA AAGTGAAGGA TCAGAAGCTC GGCGTACTCC ACAGCAGAGT 
AGGACTGAAA ATAGGACAAT GGCTACCTGG GGAGGTTCTA CGCAGTGTGC ACTAGATGAA 
GAGGATGGAG ATGAAGATGG TTATCTCTCT GATGGAGCTG GTCAGGCAGA AGAAGATGAT 
GATGATGCGT CAAGTGCGAA TGACAGCTTC TCTGTTTATC CCAATAACAA CGTACCAGAA 
AATGCATATT TTGTTAAAGA AAACAAGGAT AGGTGGAAAA ATTACCGTCC TCTTTCAGCA 
GATGGAAATT ATCGTCCATT GTCTAAGGCC AGACAACAGC AAAACATAAG TATGCGACGT 
CAGGAAAACC TTCGGTGGAT GTCTGAACTT TCATATGTGG AAGAAAAGGA ACGATGGCAA 
GAGCAGATCA ATCAGTTGAA GAAACAGCAT GAATTTAGTG TCAGCATTTG TCAAACTTTA 
ATGCAGGATC AGCAGACCCT CTCTTGTCTT CTACAGACTT TGCTTACAGG CCCTTACAGT 
ATGATGCCCA ATAATGTTGC ATCTTCACAA GTACATCTTA TTATGCATCA GCTAAACCAG 
TGTTACACTC AACTGACATG GCAGCAGAAT AACGTCCAGA GGTTGAAACA AATGTTAAAT 
GATCTTGTGC GTCAGCAAGA AGAACAACGG TATGAAGAGA AGTCATCAAG AAAGGATAGA 
GGCAGTGGTG TACCCCCACC TCCATCTCCT GTTTTCTGTC CGTTCAGCTT TCCTCCACAA 
CCTTTGAACC TGTTTAATGT ACCAGGATTT ACTAATTTTT CTTCCTTTGC TCCAGATATT 
AACTATAATC CAGTGTTTCC TTCTGGTTTT GGAGATTTTG CACACAATAT TTCTCCACAC 
AGCAGTGAGC AGCAGCAGCA ACATCCTCTA GATCATAATG CATCTGGGAA AACTGAGTAT 
ATGGCGTTCC CCAAACCTTT TGAAAGCAGT TCCTCTAATG GAGCGGAAAA ACAAAGGAGG 
AGTCACAGAC AACCTGAAGA GGAAATGGAA AAAAGATCAG CTTGGCTTAA TGATAGCCAG 
GACGTGAAAA AAGATGATCA GTCTCAGCTG AAAGCAAGTT TTGCTGTTTC AGTACAAAAC 
ATTGCTTCCA ATCATAAAAA TCAGTCTGAT ATGAGCAGAA GAAGAGAGTT TGATGAAGAG 
TCTTTGGAGA GTTTTAGTAG CATGCCTGAT CCAGTAGACC CCACTACTGT GACAAAGACA 
TTTAAATCTA GAAAAGCATC AGCACAAGCA AGTTTGGCAT CAAAAGATAA AACACCTAAA 
TCGAAGAATA AGAGGAAGAG TTCTTCTCAG CTAAAAGGCA GAATTACAAA TATTGATTAT 
GAAAGTGCCA GTGCTTCTAG TGTGTGTGAA CCCTGCAAGA GCAATAAAAG CAGACACTCT 
GAAGAGGTTG GTCATGCAAA GGTGTTCAGC AAAAGGAATC GGGAACAACT GGAAAAAATC 
ATTAAATACA GTAGATCTAC AGAAATGTCT TCAGCGCATG CTAGGAGAAT TCTGCAGCAG 
TCTAACAGAA ATGCATGCAT TGAAGCGCCA GAAACTGGTA GTGATCTTTC TATGTTTGAA 
GCTTTACGAG ACACAATTTA TTCTGAAGTG GCAACTCTTA TTTCTCAAAA TGAGTCTCGT 
CCCCACTTTC TTATTGAACT TTTCCATGAG CTTCAGCTGC TAAATACAGA TTACCTGAGG 
CAAAGAGCTC TATACGCTCT ACAGGATATA GTGACCAGAC ATTTATCTGA GAACAATGAA 
AAAGGGAAGT GCACAAAATC ACTGAATTCT GCAGCATGGA TAGCATCAAA TTCTGAACTC 
ACTCCCAGTG AAAGCCTTGC TTCTACAGAT GATGAAACTT TTGGCAAAAA CTTTTCTACA 
GAAGCATGTC AAGATTGTGA ACAAAATGAT GCAGACAATG GGAGTAGCAT GTCTACATCT 
TCAAATTTTG AACCCTTTGC CACTGATGAC CTTGGAGGTC AAAGTGCCAG TGAATGTGTT 
CCTGTGCCAC AGTCAAGTGA AGCTTCTGCT GTTCCATGTC CTCATATAGA CACTCAGCAG 
CTTGACCGGC AGATTAAAGC AATTATGAAA GAGGTCATTC CTTTTCTGAA GGAACACATG 
GATGAAGTAT GCTCTTCGCA GTTACTGACA TCAGTAAGAC GTATGGTCTT GACCCTTACA 
CAACAAAATG ATGAAAGTAA AGAGTTTGTG AAGTTCTTTC ATAAGCAGCT TGGCAGTATA 
CTTCAGGATT CACTGGCAAA ATTTGCTGGT AGAAAACTGA AAGATTGTGG AGAGGATCTT 
CTAGTGGAGA TCTCTGAAGT GTTATTTAAT GAATTAGCCT TTTTTAAACT CATGCAAGAC 
TTGGACAACA ACAGTATTTC TGTAAAGCAG AGATGTAAAA GAAAAATAGA AGCTACTGAA 
GTGATACAGT CGTATGCTAA AGAGGCAAAA AAAGGTCTCC AGGTGGATGT TTGTTCATCT 
GCTGAAGATG TCGATGAGGA CAAAGACAAG GATGAGACTG AAACTGTTAA ACAAGTACAG 
GACTCAGAAA TGTATGATGG GAATAGAGTT CCTGAAAGTA TTAGGTCTGA TGCATCTGAT 
CAGGAGGAAG ATGATGAAAG TGAAAGCGGT CCAGTGGCAA TAAGTTTATC AAAAGCAGAA 
ACCCAAGCTC TGACTAACTA TGGTAGTGGA GAAGATGAGA ATGAAGATGA AGAAATAGAA 
TTTGAGGAAG GACCTGTTGA TGTGCAGACG TCATTACAAG CCAGCAGTGA AACAACAACT 
GAAAATGAAC AGAATTCAAA CCAAGAACAA AGTAAGACAA AAAGCAGTGA GATTCTGTCA 
TCAGAACAAG AATCTGTTTA TGCTAAAGGT GAACAACATG TGGCTACAAT TTTGCCTTAT 
TACCTCAATG TAATGGAGAA TGCACCGCCT TTAACGGTCA ATACCCCAGA ATCTTTTATA 
ACAGACAGTA TGAAAACAGA AGAATCAAGT TCATCTTTAC CAGTAAATGA AACTCAAACA 
CTGGATACAA CGTGTGTAGG AAACAAATCT GCTGCAAGTT CTGAAAGCTC CATGGCTGGC 
AGCCCTGATA CAGAGTCACC TGTGTTAGTG AATGAATATG AAGCTGGTTC TGGAAATGTA 
AGTCAAAGAT CTGATGAAGA TGACTTTGTG AAAGTTGAAG ACTTGCCCCT TAAACTTGCA 
GTGTATTCAG AGGCAGATTT AATGAAGAAA ATGGCAATGG AGGCCCAAAC GAACAGTTTG 
TCTGATGAAT TACTGGGTGG AGGTGGAGCT CAAGATCAAG AATTAGTAGG AGATGCCCAA 
ACTTTGAAAG AACCTGAAAC TTTTGGAGTT CAAAGTGCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021141536.1
CDS	140..6421
Translation	MATGGGPFEEGMNDQDLPSWSNDSFDDRLNNTDWGSQQKKANRSSEKNKKKLGGEAETRLTNDISPESSPGIGRRKTRTPHSFPHARYMTQMSVPEQAELERLKQRINFSDLDQRSIGSDSQGRATAANNKRQLNENKKPFNFLSLQINTNKSKDPASCSQKKESGVSVQRKELFGAALSKDFLQNCQVSAQEDGGGEPAMDSSQIVSRLVQIRDYIAKASSMRDDLVEKNERSANVERLSHLIDHLKEQEKSYLKFLQKMLARQNEEDDVRTIDSAVGSGSVGESTSLNIDVQSEASDTTEVSFSLSIRPCIEDKLGNSASQEQVTDFDVTASPKGKNDRAALNDREIWPCRINSREHGLPSKARDPQQEAKEELENLKKQHDLLKRMLKQQEQLKALQGRQAALLALQHKAEQAIAVMDDSVVTETTGSVSGVSLTSELNEELNDLIQRFHNQLHDSQTQSVPDNRRQAESLSLTREVSQSRNSSMSERLSDEKAQLFNKMRMLQGKKQKMDKLLGELHTLRDQHLNNSSFFPASGSPQRSIDQRSTTSAASGPAGIVTVVNGESNSLASAPYPPDSLVSQNESEEDDNLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDSVNENTKEEEETEESESDSEREDPQPVTNIRNPQGISSWSDINSNSNVQCGTNNRDGRHLNTDCEINNRSAANIRTLKMSSPLDCHNREDDKDIGLPQCEDEEVEEDRVSEDSASSHRSSLGDTAGDAEFEQKINRLIAAKQKLRQLQNLAAMVQDDDPAPQTTIANASNIGDLFLGEMEETKQQPNNVRASTNKLQKDVELNEKAREKFYEAKLQQQQQELKQLQEERRKLIEIQEKIQVLQKACPDLQLSAGLGDCTANRQTSPATSTPAMNDTAGKPSLEFDESVPVGNELWSEMRRHEILREELRQRRKQLEALMAEHQRRKELAETISTVAASVKSEGSEARRTPQQSRTENRTMATWGGSTQCALDEEDGDEDGYLSDGAGQAEEDDDDASSANDSFSVYPNNNVPENAYFVKENKDRWKNYRPLSADGNYRPLSKARQQQNISMRRQENLRWMSELSYVEEKERWQEQINQLKKQHEFSVSICQTLMQDQQTLSCLLQTLLTGPYSMMPNNVASSQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNDLVRQQEEQRYEEKSSRKDRGSGVPPPPSPVFCPFSFPPQPLNLFNVPGFTNFSSFAPDINYNPVFPSGFGDFAHNISPHSSEQQQQHPLDHNASGKTEYMAFPKPFESSSSNGAEKQRRSHRQPEEEMEKRSAWLNDSQDVKKDDQSQLKASFAVSVQNIASNHKNQSDMSRRREFDEESLESFSSMPDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKNKRKSSSQLKGRITNIDYESASASSVCEPCKSNKSRHSEEVGHAKVFSKRNREQLEKIIKYSRSTEMSSAHARRILQQSNRNACIEAPETGSDLSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHLSENNEKGKCTKSLNSAAWIASNSELTPSESLASTDDETFGKNFSTEACQDCEQNDADNGSSMSTSSNFEPFATDDLGGQSASECVPVPQSSEASAVPCPHIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSISVKQRCKRKIEATEVIQSYAKEAKKGLQVDVCSSAEDVDEDKDKDETETVKQVQDSEMYDGNRVPESIRSDASDQEEDDESESGPVAISLSKAETQALTNYGSGEDENEDEEIEFEEGPVDVQTSLQASSETTTENEQNSNQEQSKTKSSEILSSEQESVYAKGEQHVATILPYYLNVMENAPPLTVNTPESFITDSMKTEESSSSLPVNETQTLDTTCVGNKSAASSESSMAGSPDTESPVLVNEYEAGSGNVSQRSDEDDFVKVEDLPLKLAVYSEADLMKKMAMEAQTNSLSDELLGGGGAQDQELVGDAQTLKEPETFGVQSA

Target ORF information:

RefSeq Version	XM_021285861.1
Organism	Columba livia(rock pigeon)
Definition	Columba livia pericentriolar material 1 (PCM1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021285861.1

ORF Insert Sequence:

ATGGCAACAG GAGGTGGTCC CTTTGAAGAA GGCATGAATG ATCAAGACTT GCCTAGCTGG 
AGCAATGACA GCTTTGATGA CCGGCTGAAC AACACGGACT GGGGAAGTCA ACAGAAGAAA 
GCAAACAGAT CTTCAGAAAA AAACAAGAAA AAGCTTGGTG GGGAAGCTGA AACAAGGCTT 
ACTAATGATA TATCTCCAGA ATCCTCACCT GGAATAGGAC GACGAAAGAC CAGAACTCCT 
CATAGTTTTC CTCATGCTCG ATACATGACC CAGATGTCTG TTCCGGAGCA GGCTGAGCTA 
GAAAGGCTTA AACAAAGAAT AAACTTCAGT GATCTGGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGCA GGGCAACAGC TGCTAACAAC AAACGTCAAC TTAATGAAAA CAAAAAACCA 
TTCAACTTCC TGTCACTGCA GATAAACACT AACAAGAGCA AAGATCCTGC CTCATGTTCC 
CAAAAAAAGG AAAGTGGGGT GTCAGTCCAA CGTAAAGAGT TGTTTGGTGC TGCTCTGAGC 
AAGGATTTCT TGCAAAATTG TCAAGTCTCT GCTCAAGAAG ATGGAGGGGG AGAACCAGCT 
ATGGATAGTA GCCAGATTGT GAGCAGACTA GTTCAAATTC GCGACTATAT TGCTAAGGCC 
AGCTCCATGC GGGATGATCT TGTAGAGAAA AATGAAAGAT CGGCCAATGT TGAGCGTTTA 
TCACACCTTA TAGATCACCT TAAAGAGCAG GAGAAATCCT ATCTGAAATT TTTGCAAAAG 
ATGCTTGCTA GACAAAATGA GGAGGATGAT GTTCGGACTA TAGATTCAGC TGTGGGATCT 
GGTTCTGTAG GTGAGAGCAC ATCGCTAAAC ATTGATGTGC AGTCTGAGGC TTCAGATACC 
ACGGAGGTAT CTTTTAGTTT GAGCATTCGG CCCTGCATTG AGGACAAACT AGGGAATTCA 
GCTTCACAAG AACAGGTTAC AGACTTTGAT GTTACTGCAA GCCCTAAAGG GAAAAATGAC 
AGAGCTGCCC TGAATGACAG GGAAATCTGG CCTTGTAGGA TTAATAGCCG AGAACATGGA 
TTGCCTTCAA AGGCTAGAGA TCCTCAACAG GAGGCTAAAG AGGAGTTGGA GAACTTGAAG 
AAGCAGCATG ATTTATTGAA AAGGATGCTA AAACAGCAAG AGCAATTAAA GGCTCTTCAA 
GGAAGACAGG CAGCTCTTCT TGCTTTGCAG CATAAAGCAG AGCAAGCAAT TGCTGTCATG 
GATGATTCTG TTGTAACAGA GACTACAGGT AGTGTTTCAG GAGTAAGCCT TACATCAGAA 
CTGAATGAAG AATTGAATGA CTTAATTCAG CGCTTTCACA ACCAACTTCA TGATTCTCAG 
ACACAGTCTG TACCAGACAA TAGAAGGCAA GCAGAAAGTC TTTCACTTAC TAGAGAGGTT 
TCACAAAGCA GAAACTCTTC AATGTCTGAA CGCCTGTCAG ATGAGAAGGC GCAGCTTTTT 
AACAAGATGC GAATGTTGCA GGGTAAAAAG CAAAAAATGG ACAAACTGTT AGGAGAACTT 
CATACACTTC GTGACCAACA TCTAAATAAC TCCTCCTTTT TTCCTGCTTC AGGTTCTCCT 
CAAAGAAGTA TTGATCAAAG AAGTACAACT TCAGCTGCTT CTGGTCCTGC AGGCATAGTA 
ACTGTTGTCA ATGGCGAATC AAATAGCCTG GCATCTGCTC CCTATCCTCC TGATTCCCTG 
GTTTCTCAGA ATGAGAGTGA AGAAGATGAC AATCTAAATC CAACAGAAAA GCTTCAGAAA 
CTAAATGAAG TTCGTAAGAG GCTGAATGAG TTACGTGAGC TAGTTCACTA CTATGAGCAA 
ACATCTGATA TGATGACAGA TTCTGTGAAT GAAAACACTA AGGAAGAAGA AGAAACCGAA 
GAATCAGAAA GTGATTCTGA ACGTGAGGAT CCACAGCCTG TTACAAATAT TAGAAATCCT 
CAAGGAATCA GTAGTTGGAG TGACATAAAT AGCAACTCAA ATGTACAGTG TGGAACTAAT 
AACAGAGATG GAAGACATCT TAATACAGAC TGTGAAATAA ATAACAGATC TGCTGCTAAC 
ATAAGGACTC TAAAAATGTC GTCACCTTTA GACTGTCATA ATAGGGAGGA TGACAAAGAT 
ATTGGCCTAC CCCAATGTGA AGATGAAGAA GTGGAAGAAG ATAGAGTTAG TGAAGATTCT 
GCATCTAGTC ACAGAAGCAG CCTGGGTGAT ACTGCTGGAG ATGCTGAGTT TGAGCAGAAG 
ATCAATAGGC TTATAGCTGC AAAACAGAAA CTTAGACAAT TACAAAACCT TGCTGCTATG 
GTGCAGGATG ATGATCCAGC ACCTCAGACA ACAATTGCAA ATGCATCTAA TATTGGTGAC 
TTGTTTTTGG GTGAGATGGA AGAGACAAAG CAGCAGCCAA ACAATGTCCG AGCTAGTACC 
AACAAATTAC AAAAAGATGT AGAACTGAAT GAAAAAGCAA GAGAGAAGTT TTATGAAGCT 
AAACTCCAGC AGCAGCAACA GGAGCTTAAG CAGTTACAAG AAGAAAGAAG AAAACTGATT 
GAAATTCAAG AAAAAATTCA GGTGTTACAG AAAGCTTGTC CTGATCTTCA ATTGTCAGCT 
GGCTTGGGTG ACTGCACAGC AAATAGACAG ACTTCACCAG CAACATCAAC TCCAGCCATG 
AATGACACAG CTGGCAAGCC TTCACTTGAA TTTGATGAAT CTGTACCAGT AGGCAATGAG 
TTATGGTCTG AGATGAGAAG ACATGAGATT TTAAGAGAAG AATTGCGACA AAGAAGAAAG 
CAACTGGAAG CTCTAATGGC TGAGCATCAG AGGAGGAAAG AGCTCGCAGA AACAATATCT 
ACTGTTGCTG CGTCTGTTAA AAGTGAAGGA TCAGAAGCTC GGCGTACTCC ACAGCAGAGT 
AGGACTGAAA ATAGGACAAT GGCTACCTGG GGAGGTTCTA CGCAGTGTGC ACTAGATGAA 
GAGGATGGAG ATGAAGATGG TTATCTCTCT GATGGAGCTG GTCAGGCAGA AGAAGATGAT 
GATGATGCGT CAAGTGCGAA TGACAGCTTC TCTGTTTATC CCAATAACAA CGTACCAGAA 
AATGCATATT TTGTTAAAGA AAACAAGGAT AGGTGGAAAA ATTACCGTCC TCTTTCAGCA 
GATGGAAATT ATCGTCCATT GTCTAAGGCC AGACAACAGC AAAACATAAG TATGCGACGT 
CAGGAAAACC TTCGGTGGAT GTCTGAACTT TCATATGTGG AAGAAAAGGA ACGATGGCAA 
GAGCAGATCA ATCAGTTGAA GAAACAGCAT GAATTTAGTG TCAGCATTTG TCAAACTTTA 
ATGCAGGATC AGCAGACCCT CTCTTGTCTT CTACAGACTT TGCTTACAGG CCCTTACAGT 
ATGATGCCCA ATAATGTTGC ATCTTCACAA GTACATCTTA TTATGCATCA GCTAAACCAG 
TGTTACACTC AACTGACATG GCAGCAGAAT AACGTCCAGA GGTTGAAACA AATGTTAAAT 
GATCTTGTGC GTCAGCAAGA AGAACAACGG TATGAAGAGA AGTCATCAAG AAAGGATAGA 
GGCAGTGGTG TACCCCCACC TCCATCTCCT GTTTTCTGTC CGTTCAGCTT TCCTCCACAA 
CCTTTGAACC TGTTTAATGT ACCAGGATTT ACTAATTTTT CTTCCTTTGC TCCAGATATT 
AACTATAATC CAGTGTTTCC TTCTGGTTTT GGAGATTTTG CACACAATAT TTCTCCACAC 
AGCAGTGAGC AGCAGCAGCA ACATCCTCTA GATCATAATG CATCTGGGAA AACTGAGTAT 
ATGGCGTTCC CCAAACCTTT TGAAAGCAGT TCCTCTAATG GAGCGGAAAA ACAAAGGAGG 
AGTCACAGAC AACCTGAAGA GGAAATGGAA AAAAGATCAG CTTGGCTTAA TGATAGCCAG 
GACGTGAAAA AAGATGATCA GTCTCAGCTG AAAGCAAGTT TTGCTGTTTC AGTACAAAAC 
ATTGCTTCCA ATCATAAAAA TCAGTCTGAT ATGAGCAGAA GAAGAGAGTT TGATGAAGAG 
TCTTTGGAGA GTTTTAGTAG CATGCCTGAT CCAGTAGACC CCACTACTGT GACAAAGACA 
TTTAAATCTA GAAAAGCATC AGCACAAGCA AGTTTGGCAT CAAAAGATAA AACACCTAAA 
TCGAAGAATA AGAGGAAGAG TTCTTCTCAG CTAAAAGGCA GAATTACAAA TATTGATTAT 
GAAAGTGCCA GTGCTTCTAG TGTGTGTGAA CCCTGCAAGA GCAATAAAAG CAGACACTCT 
GAAGAGGTTG GTCATGCAAA GGTGTTCAGC AAAAGGAATC GGGAACAACT GGAAAAAATC 
ATTAAATACA GTAGATCTAC AGAAATGTCT TCAGCGCATG CTAGGAGAAT TCTGCAGCAG 
TCTAACAGAA ATGCATGCAT TGAAGCGCCA GAAACTGGTA GTGATCTTTC TATGTTTGAA 
GCTTTACGAG ACACAATTTA TTCTGAAGTG GCAACTCTTA TTTCTCAAAA TGAGTCTCGT 
CCCCACTTTC TTATTGAACT TTTCCATGAG CTTCAGCTGC TAAATACAGA TTACCTGAGG 
CAAAGAGCTC TATACGCTCT ACAGGATATA GTGACCAGAC ATTTATCTGA GAACAATGAA 
AAAGGGAAGT GCACAAAATC ACTGAATTCT GCAGCATGGA TAGCATCAAA TTCTGAACTC 
ACTCCCAGTG AAAGCCTTGC TTCTACAGAT GATGAAACTT TTGGCAAAAA CTTTTCTACA 
GAAGCATGTC AAGATTGTGA ACAAAATGAT GCAGACAATG GGAGTAGCAT GTCTACATCT 
TCAAATTTTG AACCCTTTGC CACTGATGAC CTTGGAGGTC AAAGTGCCAG TGAATGTGTT 
CCTGTGCCAC AGTCAAGTGA AGCTTCTGCT GTTCCATGTC CTCATATAGA CACTCAGCAG 
CTTGACCGGC AGATTAAAGC AATTATGAAA GAGGTCATTC CTTTTCTGAA GGAACACATG 
GATGAAGTAT GCTCTTCGCA GTTACTGACA TCAGTAAGAC GTATGGTCTT GACCCTTACA 
CAACAAAATG ATGAAAGTAA AGAGTTTGTG AAGTTCTTTC ATAAGCAGCT TGGCAGTATA 
CTTCAGGATT CACTGGCAAA ATTTGCTGGT AGAAAACTGA AAGATTGTGG AGAGGATCTT 
CTAGTGGAGA TCTCTGAAGT GTTATTTAAT GAATTAGCCT TTTTTAAACT CATGCAAGAC 
TTGGACAACA ACAGTATTTC TGTAAAGCAG AGATGTAAAA GAAAAATAGA AGCTACTGAA 
GTGATACAGT CGTATGCTAA AGAGGCAAAA AAAGGTCTCC AGGTGGATGT TTGTTCATCT 
GCTGAAGATG TCGATGAGGA CAAAGACAAG GATGAGACTG AAACTGTTAA ACAAGTACAG 
GACTCAGAAA TGTATGATGG GAATAGAGTT CCTGAAAGTA TTAGGTCTGA TGCATCTGAT 
CAGGAGGAAG ATGATGAAAG TGAAAGCGGT CCAGTGGCAA TAAGTTTATC AAAAGCAGAA 
ACCCAAGCTC TGACTAACTA TGGTAGTGGA GAAGATGAGA ATGAAGATGA AGAAATAGAA 
TTTGAGGAAG GACCTGTTGA TGTGCAGACG TCATTACAAG CCAGCAGTGA AACAACAACT 
GAAAATGAAC AGAATTCAAA CCAAGAACAA AGTAAGACAA AAAGCAGTGA GATTCTGTCA 
TCAGAACAAG AATCTGTTTA TGCTAAAGGT GAACAACATG TGGCTACAAT TTTGCCTTAT 
TACCTCAATG TAATGGAGAA TGCACCGCCT TTAACGGTCA ATACCCCAGA ATCTTTTATA 
ACAGACAGTA TGAAAACAGA AGAATCAAGT TCATCTTTAC CAGTAAATGA AACTCAAACA 
CTGGATACAA CGTGTGTAGG AAACAAATCT GCTGCAAGTT CTGAAAGCTC CATGGCTGGC 
AGCCCTGATA CAGAGTCACC TGTGTTAGTG AATGAATATG AAGCTGGTTC TGGAAATGTA 
AGTCAAAGAT CTGATGAAGA TGACTTTGTG AAAGTTGAAG ACTTGCCCCT TAAACTTGCA 
GTGTATTCAG AGGCAGATTT AATGAAGAAA ATGGCAATGG AGGCCCAAAC GAACAGTTTG 
TCTGATGAAT TACTGGGTGG AGGTGGAGCT CAAGATCAAG AATTAGTAGG AGATGCCCAA 
ACTTTGAAAG AACCTGAAAC TTTTGGAGTT CAAAGTGCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_021285862.1 ) cDNA ORF clone, Columba livia(rock pigeon) -> XP_021141537.1 Columba livia pericentriolar material 1 (PCM1), transcript variant X9, mRNA.

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CloneID	OCo293165
Clone ID Related Accession (Same CDS sequence)	XM_021285862.1
Accession Version	XM_021285862.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-23
Organism	Columba livia(rock pigeon)
Product	pericentriolar material 1 protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004973253.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Columba livia Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACAG GAGGTGGTCC CTTTGAAGAA GGCATGAATG ATCAAGACTT GCCTAGCTGG 
AGCAATGACA GCTTTGATGA CCGGCTGAAC AACACGGACT GGGGAAGTCA ACAGAAGAAA 
GCAAACAGAT CTTCAGAAAA AAACAAGAAA AAGCTTGGTG GGGAAGCTGA AACAAGGCTT 
ACTAATGATA TATCTCCAGA ATCCTCACCT GGAATAGGAC GACGAAAGAC CAGAACTCCT 
CATAGTTTTC CTCATGCTCG ATACATGACC CAGATGTCTG TTCCGGAGCA GGCTGAGCTA 
GAAAGGCTTA AACAAAGAAT AAACTTCAGT GATCTGGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGCA GGGCAACAGC TGCTAACAAC AAACGTCAAC TTAATGAAAA CAAAAAACCA 
TTCAACTTCC TGTCACTGCA GATAAACACT AACAAGAGCA AAGATCCTGC CTCATGTTCC 
CAAAAAAAGG AAAGTGGGGT GTCAGTCCAA CGTAAAGAGT TGTTTGGTGC TGCTCTGAGC 
AAGGATTTCT TGCAAAATTG TCAAGTCTCT GCTCAAGAAG ATGGAGGGGG AGAACCAGCT 
ATGGATAGTA GCCAGGCTAG ACAAAATGAG GAGGATGATG TTCGGACTAT AGATTCAGCT 
GTGGGATCTG GTTCTGTAGG TGAGAGCACA TCGCTAAACA TTGATGTGCA GTCTGAGGCT 
TCAGATACCA CGGAGGTATC TTTTAGTTTG AGCATTCGGC CCTGCATTGA GGACAAACTA 
GGGAATTCAG CTTCACAAGA ACAGGTTACA GACTTTGATG TTACTGCAAG CCCTAAAGGG 
AAAAATGACA GAGCTGCCCT GAATGACAGG GAAATCTGGC CTTGTAGGAT TAATAGCCGA 
GAACATGGAT TGCCTTCAAA GGCTAGAGAT CCTCAACAGG AGGCTAAAGA GGAGTTGGAG 
AACTTGAAGA AGCAGCATGA TTTATTGAAA AGGATGCTAA AACAGCAAGA GCAATTAAAG 
GCTCTTCAAG GAAGACAGGC AGCTCTTCTT GCTTTGCAGC ATAAAGCAGA GCAAGCAATT 
GCTGTCATGG ATGATTCTGT TGTAACAGAG ACTACAGGTA GTGTTTCAGG AGTAAGCCTT 
ACATCAGAAC TGAATGAAGA ATTGAATGAC TTAATTCAGC GCTTTCACAA CCAACTTCAT 
GATTCTCAGA CACAGTCTGT ACCAGACAAT AGAAGGCAAG CAGAAAGTCT TTCACTTACT 
AGAGAGGTTT CACAAAGCAG AAACTCTTCA ATGTCTGAAC GCCTGTCAGA TGAGAAGGCG 
CAGCTTTTTA ACAAGATGCG AATGTTGCAG GGTAAAAAGC AAAAAATGGA CAAACTGTTA 
GGAGAACTTC ATACACTTCG TGACCAACAT CTAAATAACT CCTCCTTTTT TCCTGCTTCA 
GGTTCTCCTC AAAGAAGTAT TGATCAAAGA AGTACAACTT CAGCTGCTTC TGGTCCTGCA 
GGCATAGTAA CTGTTGTCAA TGGCGAATCA AATAGCCTGG CATCTGCTCC CTATCCTCCT 
GATTCCCTGG TTTCTCAGAA TGAGAGTGAA GAAGATGACA ATCTAAATCC AACAGAAAAG 
CTTCAGAAAC TAAATGAAGT TCGTAAGAGG CTGAATGAGT TACGTGAGCT AGTTCACTAC 
TATGAGCAAA CATCTGATAT GATGACAGAT TCTGTGAATG AAAACACTAA GGAAGAAGAA 
GAAACCGAAG AATCAGAAAG TGATTCTGAA CGTGAGGATC CACAGCCTGT TACAAATATT 
AGAAATCCTC AAGGAATCAG TAGTTGGAGT GACATAAATA GCAACTCAAA TGTACAGTGT 
GGAACTAATA ACAGAGATGG AAGACATCTT AATACAGACT GTGAAATAAA TAACAGATCT 
GCTGCTAACA TAAGGACTCT AAAAATGTCG TCACCTTTAG ACTGTCATAA TAGGGAGGAT 
GACAAAGATA TTGGCCTACC CCAATGTGAA GATGAAGAAG TGGAAGAAGA TAGAGTTAGT 
GAAGATTCTG CATCTAGTCA CAGAAGCAGC CTGGGTGATA CTGCTGGAGA TGCTGAGTTT 
GAGCAGAAGA TCAATAGGCT TATAGCTGCA AAACAGAAAC TTAGACAATT ACAAAACCTT 
GCTGCTATGG TGCAGGATGA TGATCCAGCA CCTCAGACAA CAATTGCAAA TGCATCTAAT 
ATTGGTGACT TGTTTTTGGG TGAGATGGAA GAGACAAAGC AGCAGCCAAA CAATGTCCGA 
GCTAGTACCA ACAAATTACA AAAAGATGTA GAACTGAATG AAAAAGCAAG AGAGAAGTTT 
TATGAAGCTA AACTCCAGCA GCAGCAACAG GAGCTTAAGC AGTTACAAGA AGAAAGAAGA 
AAACTGATTG AAATTCAAGA AAAAATTCAG GTGTTACAGA AAGCTTGTCC TGATCTTCAA 
TTGTCAGCTG GCTTGGGTGA CTGCACAGCA AATAGACAGA CTTCACCAGC AACATCAACT 
CCAGCCATGA ATGACACAGC TGGCAAGCCT TCACTTGAAT TTGATGAATC TGTACCAGTA 
GGCAATGAGT TATGGTCTGA GATGAGAAGA CATGAGATTT TAAGAGAAGA ATTGCGACAA 
AGAAGAAAGC AACTGGAAGC TCTAATGGCT GAGCATCAGA GGAGGAAAGA GCTCGCAGAA 
ACAATATCTA CTGTTGCTGC GTCTGTTAAA AGTGAAGGAT CAGAAGCTCG GCGTACTCCA 
CAGCAGAGTA GGACTGAAAA TAGGACAATG GCTACCTGGG GAGGTTCTAC GCAGTGTGCA 
CTAGATGAAG AGGATGGAGA TGAAGATGGT TATCTCTCTG ATGGAGCTGG TCAGGCAGAA 
GAAGATGATG ATGATGCGTC AAGTGCGAAT GACAGCTTCT CTGTTTATCC CAATAACAAC 
GTACCAGAAA ATGCATATTT TGTTAAAGAA AACAAGGATA GGTGGAAAAA TTACCGTCCT 
CTTTCAGCAG ATGGAAATTA TCGTCCATTG TCTAAGGCCA GACAACAGCA AAACATAAGT 
ATGCGACGTC AGGAAAACCT TCGGTGGATG TCTGAACTTT CATATGTGGA AGAAAAGGAA 
CGATGGCAAG AGCAGATCAA TCAGTTGAAG AAACAGCATG AATTTAGTGT CAGCATTTGT 
CAAACTTTAA TGCAGGATCA GCAGACCCTC TCTTGTCTTC TACAGACTTT GCTTACAGGC 
CCTTACAGTA TGATGCCCAA TAATGTTGCA TCTTCACAAG TACATCTTAT TATGCATCAG 
CTAAACCAGT GTTACACTCA ACTGACATGG CAGCAGAATA ACGTCCAGAG GTTGAAACAA 
ATGTTAAATG ATCTTGTGCG TCAGCAAGAA GAACAACGGT ATGAAGAGAA GTCATCAAGA 
AAGGATAGAG GCAGTGGTGT ACCCCCACCT CCATCTCCTG TTTTCTGTCC GTTCAGCTTT 
CCTCCACAAC CTTTGAACCT GTTTAATGTA CCAGGATTTA CTAATTTTTC TTCCTTTGCT 
CCAGATATTA ACTATAATCC AGTGTTTCCT TCTGGTTTTG GAGATTTTGC ACACAATATT 
TCTCCACACA GCAGTGAGCA GCAGCAGCAA CATCCTCTAG ATCATAATGC ATCTGGGAAA 
ACTGAGTATA TGGCGTTCCC CAAACCTTTT GAAAGCAGTT CCTCTAATGG AGCGGAAAAA 
CAAAGGAGGA GTCACAGACA ACCTGAAGAG GAAATGGAAA AAAGATCAGC TTGGCTTAAT 
GATAGCCAGG ACGTGAAAAA AGATGATCAG TCTCAGCTGA AAGCAAGTTT TGCTGTTTCA 
GTACAAAACA TTGCTTCCAA TCATAAAAAT CAGTCTGATA TGAGCAGAAG AAGAGAGTTT 
GATGAAGAGT CTTTGGAGAG TTTTAGTAGC ATGCCTGATC CAGTAGACCC CACTACTGTG 
ACAAAGACAT TTAAATCTAG AAAAGCATCA GCACAAGCAA GTTTGGCATC AAAAGATAAA 
ACACCTAAAT CGAAGAATAA GAGGAAGAGT TCTTCTCAGC TAAAAGGCAG AATTACAAAT 
ATTGATTATG AAAGTGCCAG TGCTTCTAGT GTGTGTGAAC CCTGCAAGAG CAATAAAAGC 
AGACACTCTG AAGAGGTTGG TCATGCAAAG GTGTTCAGCA AAAGGAATCG GGAACAACTG 
GAAAAAATCA TTAAATACAG TAGATCTACA GAAATGTCTT CAGCGCATGC TAGGAGAATT 
CTGCAGCAGT CTAACAGAAA TGCATGCATT GAAGCGCCAG AAACTGGTAG TGATCTTTCT 
ATGTTTGAAG CTTTACGAGA CACAATTTAT TCTGAAGTGG CAACTCTTAT TTCTCAAAAT 
GAGTCTCGTC CCCACTTTCT TATTGAACTT TTCCATGAGC TTCAGCTGCT AAATACAGAT 
TACCTGAGGC AAAGAGCTCT ATACGCTCTA CAGGATATAG TGACCAGACA TTTATCTGAG 
AACAATGAAA AAGGGAAGTG CACAAAATCA CTGAATTCTG CAGCATGGAT AGCATCAAAT 
TCTGAACTCA CTCCCAGTGA AAGCCTTGCT TCTACAGATG ATGAAACTTT TGGCAAAAAC 
TTTTCTACAG AAGCATGTCA AGATTGTGAA CAAAATGATG CAGACAATGG GAGTAGCATG 
TCTACATCTT CAAATTTTGA ACCCTTTGCC ACTGATGACC TTGGCAACAC AGTGATTCAC 
TTAGATCAAG CTTTGGCTAG GATGAGGGAG TATGAGCGTA TGAAAATTGA AGCTGAAAGT 
ACCCTTGACT CTGAGGGCTG CTCTAGTAAT TTTCAGGGTG CTTCTGCTGC TAAATTAGAA 
GGAGGTCAAA GTGCCAGTGA ATGTGTTCCT GTGCCACAGT CAAGTGAAGC TTCTGCTGTT 
CCATGTCCTC ATATAGACAC TCAGCAGCTT GACCGGCAGA TTAAAGCAAT TATGAAAGAG 
GTCATTCCTT TTCTGAAGGA ACACATGGAT GAAGTATGCT CTTCGCAGTT ACTGACATCA 
GTAAGACGTA TGGTCTTGAC CCTTACACAA CAAAATGATG AAAGTAAAGA GTTTGTGAAG 
TTCTTTCATA AGCAGCTTGG CAGTATACTT CAGGATTCAC TGGCAAAATT TGCTGGTAGA 
AAACTGAAAG ATTGTGGAGA GGATCTTCTA GTGGAGATCT CTGAAGTGTT ATTTAATGAA 
TTAGCCTTTT TTAAACTCAT GCAAGACTTG GACAACAACA GTATTTCTGT AAAGCAGAGA 
TGTAAAAGAA AAATAGAAGC TACTGAAGTG ATACAGTCGT ATGCTAAAGA GGCAAAAAAA 
GGTCTCCAGG TGGATGTTTG TTCATCTGCT GAAGATGTCG ATGAGGACAA AGACAAGGAT 
GAGACTGAAA CTGTTAAACA AGTACAGGAC TCAGAAATGT ATGATGGGAA TAGAGTTCCT 
GAAAGTATTA GGTCTGATGC ATCTGATCAG GAGGAAGATG ATGAAAGTGA AAGCGGTCCA 
GTGGCAATAA GTTTATCAAA AGCAGAAACC CAAGCTCTGA CTAACTATGG TAGTGGAGAA 
GATGAGAATG AAGATGAAGA AATAGAATTT GAGGAAGGAC CTGTTGATGT GCAGACGTCA 
TTACAAGCCA GCAGTGAAAC AACAACTGAA AATGAACAGA ATTCAAACCA AGAACAAAGT 
AAGACAAAAA GCAGTGAGAT TCTGTCATCA GAACAAGAAT CTGTTTATGC TAAAGGTGAA 
CAACATGTGG CTACAATTTT GCCTTATTAC CTCAATGTAA TGGAGAATGC ACCGCCTTTA 
ACGGTCAATA CCCCAGAATC TTTTATAACA GACAGTATGA AAACAGAAGA ATCAAGTTCA 
TCTTTACCAG TAAATGAAAC TCAAACACTG GATACAACGT GTGTAGGAAA CAAATCTGCT 
GCAAGTTCTG AAAGCTCCAT GGCTGGCAGC CCTGATACAG AGTCACCTGT GTTAGTGAAT 
GAATATGAAG CTGGTTCTGG AAATGTAAGT CAAAGATCTG ATGAAGATGA CTTTGTGAAA 
GTTGAAGACT TGCCCCTTAA ACTTGCAGTG TATTCAGAGG CAGATTTAAT GAAGAAAATG 
GCAATGGAGG CCCAAACGAA CAGTTTGTCT GATGAATTAC TGGGTGGAGG TGGAGCTCAA 
GATCAAGAAT TAGTAGGAGA TGCCCAAACT TTGAAAGAAC CTGAAACTTT TGGAGTTCAA 
AGTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021141537.1
CDS	140..6388
Translation	MATGGGPFEEGMNDQDLPSWSNDSFDDRLNNTDWGSQQKKANRSSEKNKKKLGGEAETRLTNDISPESSPGIGRRKTRTPHSFPHARYMTQMSVPEQAELERLKQRINFSDLDQRSIGSDSQGRATAANNKRQLNENKKPFNFLSLQINTNKSKDPASCSQKKESGVSVQRKELFGAALSKDFLQNCQVSAQEDGGGEPAMDSSQARQNEEDDVRTIDSAVGSGSVGESTSLNIDVQSEASDTTEVSFSLSIRPCIEDKLGNSASQEQVTDFDVTASPKGKNDRAALNDREIWPCRINSREHGLPSKARDPQQEAKEELENLKKQHDLLKRMLKQQEQLKALQGRQAALLALQHKAEQAIAVMDDSVVTETTGSVSGVSLTSELNEELNDLIQRFHNQLHDSQTQSVPDNRRQAESLSLTREVSQSRNSSMSERLSDEKAQLFNKMRMLQGKKQKMDKLLGELHTLRDQHLNNSSFFPASGSPQRSIDQRSTTSAASGPAGIVTVVNGESNSLASAPYPPDSLVSQNESEEDDNLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDSVNENTKEEEETEESESDSEREDPQPVTNIRNPQGISSWSDINSNSNVQCGTNNRDGRHLNTDCEINNRSAANIRTLKMSSPLDCHNREDDKDIGLPQCEDEEVEEDRVSEDSASSHRSSLGDTAGDAEFEQKINRLIAAKQKLRQLQNLAAMVQDDDPAPQTTIANASNIGDLFLGEMEETKQQPNNVRASTNKLQKDVELNEKAREKFYEAKLQQQQQELKQLQEERRKLIEIQEKIQVLQKACPDLQLSAGLGDCTANRQTSPATSTPAMNDTAGKPSLEFDESVPVGNELWSEMRRHEILREELRQRRKQLEALMAEHQRRKELAETISTVAASVKSEGSEARRTPQQSRTENRTMATWGGSTQCALDEEDGDEDGYLSDGAGQAEEDDDDASSANDSFSVYPNNNVPENAYFVKENKDRWKNYRPLSADGNYRPLSKARQQQNISMRRQENLRWMSELSYVEEKERWQEQINQLKKQHEFSVSICQTLMQDQQTLSCLLQTLLTGPYSMMPNNVASSQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNDLVRQQEEQRYEEKSSRKDRGSGVPPPPSPVFCPFSFPPQPLNLFNVPGFTNFSSFAPDINYNPVFPSGFGDFAHNISPHSSEQQQQHPLDHNASGKTEYMAFPKPFESSSSNGAEKQRRSHRQPEEEMEKRSAWLNDSQDVKKDDQSQLKASFAVSVQNIASNHKNQSDMSRRREFDEESLESFSSMPDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKNKRKSSSQLKGRITNIDYESASASSVCEPCKSNKSRHSEEVGHAKVFSKRNREQLEKIIKYSRSTEMSSAHARRILQQSNRNACIEAPETGSDLSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHLSENNEKGKCTKSLNSAAWIASNSELTPSESLASTDDETFGKNFSTEACQDCEQNDADNGSSMSTSSNFEPFATDDLGNTVIHLDQALARMREYERMKIEAESTLDSEGCSSNFQGASAAKLEGGQSASECVPVPQSSEASAVPCPHIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSISVKQRCKRKIEATEVIQSYAKEAKKGLQVDVCSSAEDVDEDKDKDETETVKQVQDSEMYDGNRVPESIRSDASDQEEDDESESGPVAISLSKAETQALTNYGSGEDENEDEEIEFEEGPVDVQTSLQASSETTTENEQNSNQEQSKTKSSEILSSEQESVYAKGEQHVATILPYYLNVMENAPPLTVNTPESFITDSMKTEESSSSLPVNETQTLDTTCVGNKSAASSESSMAGSPDTESPVLVNEYEAGSGNVSQRSDEDDFVKVEDLPLKLAVYSEADLMKKMAMEAQTNSLSDELLGGGGAQDQELVGDAQTLKEPETFGVQSA

Target ORF information:

RefSeq Version	XM_021285862.1
Organism	Columba livia(rock pigeon)
Definition	Columba livia pericentriolar material 1 (PCM1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021285862.1

ORF Insert Sequence:

ATGGCAACAG GAGGTGGTCC CTTTGAAGAA GGCATGAATG ATCAAGACTT GCCTAGCTGG 
AGCAATGACA GCTTTGATGA CCGGCTGAAC AACACGGACT GGGGAAGTCA ACAGAAGAAA 
GCAAACAGAT CTTCAGAAAA AAACAAGAAA AAGCTTGGTG GGGAAGCTGA AACAAGGCTT 
ACTAATGATA TATCTCCAGA ATCCTCACCT GGAATAGGAC GACGAAAGAC CAGAACTCCT 
CATAGTTTTC CTCATGCTCG ATACATGACC CAGATGTCTG TTCCGGAGCA GGCTGAGCTA 
GAAAGGCTTA AACAAAGAAT AAACTTCAGT GATCTGGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGCA GGGCAACAGC TGCTAACAAC AAACGTCAAC TTAATGAAAA CAAAAAACCA 
TTCAACTTCC TGTCACTGCA GATAAACACT AACAAGAGCA AAGATCCTGC CTCATGTTCC 
CAAAAAAAGG AAAGTGGGGT GTCAGTCCAA CGTAAAGAGT TGTTTGGTGC TGCTCTGAGC 
AAGGATTTCT TGCAAAATTG TCAAGTCTCT GCTCAAGAAG ATGGAGGGGG AGAACCAGCT 
ATGGATAGTA GCCAGGCTAG ACAAAATGAG GAGGATGATG TTCGGACTAT AGATTCAGCT 
GTGGGATCTG GTTCTGTAGG TGAGAGCACA TCGCTAAACA TTGATGTGCA GTCTGAGGCT 
TCAGATACCA CGGAGGTATC TTTTAGTTTG AGCATTCGGC CCTGCATTGA GGACAAACTA 
GGGAATTCAG CTTCACAAGA ACAGGTTACA GACTTTGATG TTACTGCAAG CCCTAAAGGG 
AAAAATGACA GAGCTGCCCT GAATGACAGG GAAATCTGGC CTTGTAGGAT TAATAGCCGA 
GAACATGGAT TGCCTTCAAA GGCTAGAGAT CCTCAACAGG AGGCTAAAGA GGAGTTGGAG 
AACTTGAAGA AGCAGCATGA TTTATTGAAA AGGATGCTAA AACAGCAAGA GCAATTAAAG 
GCTCTTCAAG GAAGACAGGC AGCTCTTCTT GCTTTGCAGC ATAAAGCAGA GCAAGCAATT 
GCTGTCATGG ATGATTCTGT TGTAACAGAG ACTACAGGTA GTGTTTCAGG AGTAAGCCTT 
ACATCAGAAC TGAATGAAGA ATTGAATGAC TTAATTCAGC GCTTTCACAA CCAACTTCAT 
GATTCTCAGA CACAGTCTGT ACCAGACAAT AGAAGGCAAG CAGAAAGTCT TTCACTTACT 
AGAGAGGTTT CACAAAGCAG AAACTCTTCA ATGTCTGAAC GCCTGTCAGA TGAGAAGGCG 
CAGCTTTTTA ACAAGATGCG AATGTTGCAG GGTAAAAAGC AAAAAATGGA CAAACTGTTA 
GGAGAACTTC ATACACTTCG TGACCAACAT CTAAATAACT CCTCCTTTTT TCCTGCTTCA 
GGTTCTCCTC AAAGAAGTAT TGATCAAAGA AGTACAACTT CAGCTGCTTC TGGTCCTGCA 
GGCATAGTAA CTGTTGTCAA TGGCGAATCA AATAGCCTGG CATCTGCTCC CTATCCTCCT 
GATTCCCTGG TTTCTCAGAA TGAGAGTGAA GAAGATGACA ATCTAAATCC AACAGAAAAG 
CTTCAGAAAC TAAATGAAGT TCGTAAGAGG CTGAATGAGT TACGTGAGCT AGTTCACTAC 
TATGAGCAAA CATCTGATAT GATGACAGAT TCTGTGAATG AAAACACTAA GGAAGAAGAA 
GAAACCGAAG AATCAGAAAG TGATTCTGAA CGTGAGGATC CACAGCCTGT TACAAATATT 
AGAAATCCTC AAGGAATCAG TAGTTGGAGT GACATAAATA GCAACTCAAA TGTACAGTGT 
GGAACTAATA ACAGAGATGG AAGACATCTT AATACAGACT GTGAAATAAA TAACAGATCT 
GCTGCTAACA TAAGGACTCT AAAAATGTCG TCACCTTTAG ACTGTCATAA TAGGGAGGAT 
GACAAAGATA TTGGCCTACC CCAATGTGAA GATGAAGAAG TGGAAGAAGA TAGAGTTAGT 
GAAGATTCTG CATCTAGTCA CAGAAGCAGC CTGGGTGATA CTGCTGGAGA TGCTGAGTTT 
GAGCAGAAGA TCAATAGGCT TATAGCTGCA AAACAGAAAC TTAGACAATT ACAAAACCTT 
GCTGCTATGG TGCAGGATGA TGATCCAGCA CCTCAGACAA CAATTGCAAA TGCATCTAAT 
ATTGGTGACT TGTTTTTGGG TGAGATGGAA GAGACAAAGC AGCAGCCAAA CAATGTCCGA 
GCTAGTACCA ACAAATTACA AAAAGATGTA GAACTGAATG AAAAAGCAAG AGAGAAGTTT 
TATGAAGCTA AACTCCAGCA GCAGCAACAG GAGCTTAAGC AGTTACAAGA AGAAAGAAGA 
AAACTGATTG AAATTCAAGA AAAAATTCAG GTGTTACAGA AAGCTTGTCC TGATCTTCAA 
TTGTCAGCTG GCTTGGGTGA CTGCACAGCA AATAGACAGA CTTCACCAGC AACATCAACT 
CCAGCCATGA ATGACACAGC TGGCAAGCCT TCACTTGAAT TTGATGAATC TGTACCAGTA 
GGCAATGAGT TATGGTCTGA GATGAGAAGA CATGAGATTT TAAGAGAAGA ATTGCGACAA 
AGAAGAAAGC AACTGGAAGC TCTAATGGCT GAGCATCAGA GGAGGAAAGA GCTCGCAGAA 
ACAATATCTA CTGTTGCTGC GTCTGTTAAA AGTGAAGGAT CAGAAGCTCG GCGTACTCCA 
CAGCAGAGTA GGACTGAAAA TAGGACAATG GCTACCTGGG GAGGTTCTAC GCAGTGTGCA 
CTAGATGAAG AGGATGGAGA TGAAGATGGT TATCTCTCTG ATGGAGCTGG TCAGGCAGAA 
GAAGATGATG ATGATGCGTC AAGTGCGAAT GACAGCTTCT CTGTTTATCC CAATAACAAC 
GTACCAGAAA ATGCATATTT TGTTAAAGAA AACAAGGATA GGTGGAAAAA TTACCGTCCT 
CTTTCAGCAG ATGGAAATTA TCGTCCATTG TCTAAGGCCA GACAACAGCA AAACATAAGT 
ATGCGACGTC AGGAAAACCT TCGGTGGATG TCTGAACTTT CATATGTGGA AGAAAAGGAA 
CGATGGCAAG AGCAGATCAA TCAGTTGAAG AAACAGCATG AATTTAGTGT CAGCATTTGT 
CAAACTTTAA TGCAGGATCA GCAGACCCTC TCTTGTCTTC TACAGACTTT GCTTACAGGC 
CCTTACAGTA TGATGCCCAA TAATGTTGCA TCTTCACAAG TACATCTTAT TATGCATCAG 
CTAAACCAGT GTTACACTCA ACTGACATGG CAGCAGAATA ACGTCCAGAG GTTGAAACAA 
ATGTTAAATG ATCTTGTGCG TCAGCAAGAA GAACAACGGT ATGAAGAGAA GTCATCAAGA 
AAGGATAGAG GCAGTGGTGT ACCCCCACCT CCATCTCCTG TTTTCTGTCC GTTCAGCTTT 
CCTCCACAAC CTTTGAACCT GTTTAATGTA CCAGGATTTA CTAATTTTTC TTCCTTTGCT 
CCAGATATTA ACTATAATCC AGTGTTTCCT TCTGGTTTTG GAGATTTTGC ACACAATATT 
TCTCCACACA GCAGTGAGCA GCAGCAGCAA CATCCTCTAG ATCATAATGC ATCTGGGAAA 
ACTGAGTATA TGGCGTTCCC CAAACCTTTT GAAAGCAGTT CCTCTAATGG AGCGGAAAAA 
CAAAGGAGGA GTCACAGACA ACCTGAAGAG GAAATGGAAA AAAGATCAGC TTGGCTTAAT 
GATAGCCAGG ACGTGAAAAA AGATGATCAG TCTCAGCTGA AAGCAAGTTT TGCTGTTTCA 
GTACAAAACA TTGCTTCCAA TCATAAAAAT CAGTCTGATA TGAGCAGAAG AAGAGAGTTT 
GATGAAGAGT CTTTGGAGAG TTTTAGTAGC ATGCCTGATC CAGTAGACCC CACTACTGTG 
ACAAAGACAT TTAAATCTAG AAAAGCATCA GCACAAGCAA GTTTGGCATC AAAAGATAAA 
ACACCTAAAT CGAAGAATAA GAGGAAGAGT TCTTCTCAGC TAAAAGGCAG AATTACAAAT 
ATTGATTATG AAAGTGCCAG TGCTTCTAGT GTGTGTGAAC CCTGCAAGAG CAATAAAAGC 
AGACACTCTG AAGAGGTTGG TCATGCAAAG GTGTTCAGCA AAAGGAATCG GGAACAACTG 
GAAAAAATCA TTAAATACAG TAGATCTACA GAAATGTCTT CAGCGCATGC TAGGAGAATT 
CTGCAGCAGT CTAACAGAAA TGCATGCATT GAAGCGCCAG AAACTGGTAG TGATCTTTCT 
ATGTTTGAAG CTTTACGAGA CACAATTTAT TCTGAAGTGG CAACTCTTAT TTCTCAAAAT 
GAGTCTCGTC CCCACTTTCT TATTGAACTT TTCCATGAGC TTCAGCTGCT AAATACAGAT 
TACCTGAGGC AAAGAGCTCT ATACGCTCTA CAGGATATAG TGACCAGACA TTTATCTGAG 
AACAATGAAA AAGGGAAGTG CACAAAATCA CTGAATTCTG CAGCATGGAT AGCATCAAAT 
TCTGAACTCA CTCCCAGTGA AAGCCTTGCT TCTACAGATG ATGAAACTTT TGGCAAAAAC 
TTTTCTACAG AAGCATGTCA AGATTGTGAA CAAAATGATG CAGACAATGG GAGTAGCATG 
TCTACATCTT CAAATTTTGA ACCCTTTGCC ACTGATGACC TTGGCAACAC AGTGATTCAC 
TTAGATCAAG CTTTGGCTAG GATGAGGGAG TATGAGCGTA TGAAAATTGA AGCTGAAAGT 
ACCCTTGACT CTGAGGGCTG CTCTAGTAAT TTTCAGGGTG CTTCTGCTGC TAAATTAGAA 
GGAGGTCAAA GTGCCAGTGA ATGTGTTCCT GTGCCACAGT CAAGTGAAGC TTCTGCTGTT 
CCATGTCCTC ATATAGACAC TCAGCAGCTT GACCGGCAGA TTAAAGCAAT TATGAAAGAG 
GTCATTCCTT TTCTGAAGGA ACACATGGAT GAAGTATGCT CTTCGCAGTT ACTGACATCA 
GTAAGACGTA TGGTCTTGAC CCTTACACAA CAAAATGATG AAAGTAAAGA GTTTGTGAAG 
TTCTTTCATA AGCAGCTTGG CAGTATACTT CAGGATTCAC TGGCAAAATT TGCTGGTAGA 
AAACTGAAAG ATTGTGGAGA GGATCTTCTA GTGGAGATCT CTGAAGTGTT ATTTAATGAA 
TTAGCCTTTT TTAAACTCAT GCAAGACTTG GACAACAACA GTATTTCTGT AAAGCAGAGA 
TGTAAAAGAA AAATAGAAGC TACTGAAGTG ATACAGTCGT ATGCTAAAGA GGCAAAAAAA 
GGTCTCCAGG TGGATGTTTG TTCATCTGCT GAAGATGTCG ATGAGGACAA AGACAAGGAT 
GAGACTGAAA CTGTTAAACA AGTACAGGAC TCAGAAATGT ATGATGGGAA TAGAGTTCCT 
GAAAGTATTA GGTCTGATGC ATCTGATCAG GAGGAAGATG ATGAAAGTGA AAGCGGTCCA 
GTGGCAATAA GTTTATCAAA AGCAGAAACC CAAGCTCTGA CTAACTATGG TAGTGGAGAA 
GATGAGAATG AAGATGAAGA AATAGAATTT GAGGAAGGAC CTGTTGATGT GCAGACGTCA 
TTACAAGCCA GCAGTGAAAC AACAACTGAA AATGAACAGA ATTCAAACCA AGAACAAAGT 
AAGACAAAAA GCAGTGAGAT TCTGTCATCA GAACAAGAAT CTGTTTATGC TAAAGGTGAA 
CAACATGTGG CTACAATTTT GCCTTATTAC CTCAATGTAA TGGAGAATGC ACCGCCTTTA 
ACGGTCAATA CCCCAGAATC TTTTATAACA GACAGTATGA AAACAGAAGA ATCAAGTTCA 
TCTTTACCAG TAAATGAAAC TCAAACACTG GATACAACGT GTGTAGGAAA CAAATCTGCT 
GCAAGTTCTG AAAGCTCCAT GGCTGGCAGC CCTGATACAG AGTCACCTGT GTTAGTGAAT 
GAATATGAAG CTGGTTCTGG AAATGTAAGT CAAAGATCTG ATGAAGATGA CTTTGTGAAA 
GTTGAAGACT TGCCCCTTAA ACTTGCAGTG TATTCAGAGG CAGATTTAAT GAAGAAAATG 
GCAATGGAGG CCCAAACGAA CAGTTTGTCT GATGAATTAC TGGGTGGAGG TGGAGCTCAA 
GATCAAGAAT TAGTAGGAGA TGCCCAAACT TTGAAAGAAC CTGAAACTTT TGGAGTTCAA 
AGTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_021285863.1 ) cDNA ORF clone, Columba livia(rock pigeon) -> XP_021141538.1 Columba livia pericentriolar material 1 (PCM1), transcript variant X10, mRNA.

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CloneID	OCo293166
Clone ID Related Accession (Same CDS sequence)	XM_021285863.1
Accession Version	XM_021285863.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-23
Organism	Columba livia(rock pigeon)
Product	pericentriolar material 1 protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004973253.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Columba livia Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACAG GAGGTGGTCC CTTTGAAGAA GGCATGAATG ATCAAGACTT GCCTAGCTGG 
AGCAATGACA GCTTTGATGA CCGGCTGAAC AACACGGACT GGGGAAGTCA ACAGAAGAAA 
GCAAACAGAT CTTCAGAAAA AAACAAGAAA AAGCTTGGTG GGGAAGCTGA AACAAGGCTT 
ACTAATGATA TATCTCCAGA ATCCTCACCT GGAATAGGAC GACGAAAGAC CAGAACTCCT 
CATAGTTTTC CTCATGCTCG ATACATGACC CAGATGTCTG TTCCGGAGCA GGCTGAGCTA 
GAAAGGCTTA AACAAAGAAT AAACTTCAGT GATCTGGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGCA GGGCAACAGC TGCTAACAAC AAACGTCAAC TTAATGAAAA CAAAAAACCA 
TTCAACTTCC TGTCACTGCA GATAAACACT AACAAGAGCA AAGATCCTGC CTCATGTTCC 
CAAAAAAAGG AAAGTGGGGT GTCAGTCCAA CGTAAAGAGT TGTTTGGTGC TGCTCTGAGC 
AAGGATTTCT TGCAAAATTG TCAAGTCTCT GCTCAAGAAG ATGGAGGGGG AGAACCAGCT 
ATGGATAGTA GCCAGATTGT GAGCAGACTA GTTCAAATTC GCGACTATAT TGCTAAGGCC 
AGCTCCATGC GGGATGATCT TGTAGAGAAA AATGAAAGAT CGGCCAATGT TGAGCGTTTA 
TCACACCTTA TAGATCACCT TAAAGAGCAG GAGAAATCCT ATCTGAAATT TTTGCAAAAG 
ATGCTTGCTA GACAAAATGA GGAGGATGAT GTTCGGACTA TAGATTCAGC TGTGGGATCT 
GGTTCTGTAG GTGAGAGCAC ATCGCTAAAC ATTGATGTGC AGTCTGAGGC TTCAGATACC 
ACGGCTAGAG ATCCTCAACA GGAGGCTAAA GAGGAGTTGG AGAACTTGAA GAAGCAGCAT 
GATTTATTGA AAAGGATGCT AAAACAGCAA GAGCAATTAA AGGCTCTTCA AGGAAGACAG 
GCAGCTCTTC TTGCTTTGCA GCATAAAGCA GAGCAAGCAA TTGCTGTCAT GGATGATTCT 
GTTGTAACAG AGACTACAGG TAGTGTTTCA GGAGTAAGCC TTACATCAGA ACTGAATGAA 
GAATTGAATG ACTTAATTCA GCGCTTTCAC AACCAACTTC ATGATTCTCA GACACAGTCT 
GTACCAGACA ATAGAAGGCA AGCAGAAAGT CTTTCACTTA CTAGAGAGGT TTCACAAAGC 
AGAAACTCTT CAATGTCTGA ACGCCTGTCA GATGAGAAGG CGCAGCTTTT TAACAAGATG 
CGAATGTTGC AGGGTAAAAA GCAAAAAATG GACAAACTGT TAGGAGAACT TCATACACTT 
CGTGACCAAC ATCTAAATAA CTCCTCCTTT TTTCCTGCTT CAGGTTCTCC TCAAAGAAGT 
ATTGATCAAA GAAGTACAAC TTCAGCTGCT TCTGGTCCTG CAGGCATAGT AACTGTTGTC 
AATGGCGAAT CAAATAGCCT GGCATCTGCT CCCTATCCTC CTGATTCCCT GGTTTCTCAG 
AATGAGAGTG AAGAAGATGA CAATCTAAAT CCAACAGAAA AGCTTCAGAA ACTAAATGAA 
GTTCGTAAGA GGCTGAATGA GTTACGTGAG CTAGTTCACT ACTATGAGCA AACATCTGAT 
ATGATGACAG ATTCTGTGAA TGAAAACACT AAGGAAGAAG AAGAAACCGA AGAATCAGAA 
AGTGATTCTG AACGTGAGGA TCCACAGCCT GTTACAAATA TTAGAAATCC TCAAGGAATC 
AGTAGTTGGA GTGACATAAA TAGCAACTCA AATGTACAGT GTGGAACTAA TAACAGAGAT 
GGAAGACATC TTAATACAGA CTGTGAAATA AATAACAGAT CTGCTGCTAA CATAAGGACT 
CTAAAAATGT CGTCACCTTT AGACTGTCAT AATAGGGAGG ATGACAAAGA TATTGGCCTA 
CCCCAATGTG AAGATGAAGA AGTGGAAGAA GATAGAGTTA GTGAAGATTC TGCATCTAGT 
CACAGAAGCA GCCTGGGTGA TACTGCTGGA GATGCTGAGT TTGAGCAGAA GATCAATAGG 
CTTATAGCTG CAAAACAGAA ACTTAGACAA TTACAAAACC TTGCTGCTAT GGTGCAGGAT 
GATGATCCAG CACCTCAGAC AACAATTGCA AATGCATCTA ATATTGGTGA CTTGTTTTTG 
GGTGAGATGG AAGAGACAAA GCAGCAGCCA AACAATGTCC GAGCTAGTAC CAACAAATTA 
CAAAAAGATG TAGAACTGAA TGAAAAAGCA AGAGAGAAGT TTTATGAAGC TAAACTCCAG 
CAGCAGCAAC AGGAGCTTAA GCAGTTACAA GAAGAAAGAA GAAAACTGAT TGAAATTCAA 
GAAAAAATTC AGGTGTTACA GAAAGCTTGT CCTGATCTTC AATTGTCAGC TGGCTTGGGT 
GACTGCACAG CAAATAGACA GACTTCACCA GCAACATCAA CTCCAGCCAT GAATGACACA 
GCTGGCAAGC CTTCACTTGA ATTTGATGAA TCTGTACCAG TAGGCAATGA GTTATGGTCT 
GAGATGAGAA GACATGAGAT TTTAAGAGAA GAATTGCGAC AAAGAAGAAA GCAACTGGAA 
GCTCTAATGG CTGAGCATCA GAGGAGGAAA GAGCTCGCAG AAACAATATC TACTGTTGCT 
GCGTCTGTTA AAAGTGAAGG ATCAGAAGCT CGGCGTACTC CACAGCAGAG TAGGACTGAA 
AATAGGACAA TGGCTACCTG GGGAGGTTCT ACGCAGTGTG CACTAGATGA AGAGGATGGA 
GATGAAGATG GTTATCTCTC TGATGGAGCT GGTCAGGCAG AAGAAGATGA TGATGATGCG 
TCAAGTGCGA ATGACAGCTT CTCTGTTTAT CCCAATAACA ACGTACCAGA AAATGCATAT 
TTTGTTAAAG AAAACAAGGA TAGGTGGAAA AATTACCGTC CTCTTTCAGC AGATGGAAAT 
TATCGTCCAT TGTCTAAGGC CAGACAACAG CAAAACATAA GTATGCGACG TCAGGAAAAC 
CTTCGGTGGA TGTCTGAACT TTCATATGTG GAAGAAAAGG AACGATGGCA AGAGCAGATC 
AATCAGTTGA AGAAACAGCA TGAATTTAGT GTCAGCATTT GTCAAACTTT AATGCAGGAT 
CAGCAGACCC TCTCTTGTCT TCTACAGACT TTGCTTACAG GCCCTTACAG TATGATGCCC 
AATAATGTTG CATCTTCACA AGTACATCTT ATTATGCATC AGCTAAACCA GTGTTACACT 
CAACTGACAT GGCAGCAGAA TAACGTCCAG AGGTTGAAAC AAATGTTAAA TGATCTTGTG 
CGTCAGCAAG AAGAACAACG GTATGAAGAG AAGTCATCAA GAAAGGATAG AGGCAGTGGT 
GTACCCCCAC CTCCATCTCC TGTTTTCTGT CCGTTCAGCT TTCCTCCACA ACCTTTGAAC 
CTGTTTAATG TACCAGGATT TACTAATTTT TCTTCCTTTG CTCCAGATAT TAACTATAAT 
CCAGTGTTTC CTTCTGGTTT TGGAGATTTT GCACACAATA TTTCTCCACA CAGCAGTGAG 
CAGCAGCAGC AACATCCTCT AGATCATAAT GCATCTGGGA AAACTGAGTA TATGGCGTTC 
CCCAAACCTT TTGAAAGCAG TTCCTCTAAT GGAGCGGAAA AACAAAGGAG GAGTCACAGA 
CAACCTGAAG AGGAAATGGA AAAAAGATCA GCTTGGCTTA ATGATAGCCA GGACGTGAAA 
AAAGATGATC AGTCTCAGCT GAAAGCAAGT TTTGCTGTTT CAGTACAAAA CATTGCTTCC 
AATCATAAAA ATCAGTCTGA TATGAGCAGA AGAAGAGAGT TTGATGAAGA GTCTTTGGAG 
AGTTTTAGTA GCATGCCTGA TCCAGTAGAC CCCACTACTG TGACAAAGAC ATTTAAATCT 
AGAAAAGCAT CAGCACAAGC AAGTTTGGCA TCAAAAGATA AAACACCTAA ATCGAAGAAT 
AAGAGGAAGA GTTCTTCTCA GCTAAAAGGC AGAATTACAA ATATTGATTA TGAAAGTGCC 
AGTGCTTCTA GTGTGTGTGA ACCCTGCAAG AGCAATAAAA GCAGACACTC TGAAGAGGTT 
GGTCATGCAA AGGTGTTCAG CAAAAGGAAT CGGGAACAAC TGGAAAAAAT CATTAAATAC 
AGTAGATCTA CAGAAATGTC TTCAGCGCAT GCTAGGAGAA TTCTGCAGCA GTCTAACAGA 
AATGCATGCA TTGAAGCGCC AGAAACTGGT AGTGATCTTT CTATGTTTGA AGCTTTACGA 
GACACAATTT ATTCTGAAGT GGCAACTCTT ATTTCTCAAA ATGAGTCTCG TCCCCACTTT 
CTTATTGAAC TTTTCCATGA GCTTCAGCTG CTAAATACAG ATTACCTGAG GCAAAGAGCT 
CTATACGCTC TACAGGATAT AGTGACCAGA CATTTATCTG AGAACAATGA AAAAGGGAAG 
TGCACAAAAT CACTGAATTC TGCAGCATGG ATAGCATCAA ATTCTGAACT CACTCCCAGT 
GAAAGCCTTG CTTCTACAGA TGATGAAACT TTTGGCAAAA ACTTTTCTAC AGAAGCATGT 
CAAGATTGTG AACAAAATGA TGCAGACAAT GGGAGTAGCA TGTCTACATC TTCAAATTTT 
GAACCCTTTG CCACTGATGA CCTTGGCAAC ACAGTGATTC ACTTAGATCA AGCTTTGGCT 
AGGATGAGGG AGTATGAGCG TATGAAAATT GAAGCTGAAA GTACCCTTGA CTCTGAGGGC 
TGCTCTAGTA ATTTTCAGGG TGCTTCTGCT GCTAAATTAG AAGGAGGTCA AAGTGCCAGT 
GAATGTGTTC CTGTGCCACA GTCAAGTGAA GCTTCTGCTG TTCCATGTCC TCATATAGAC 
ACTCAGCAGC TTGACCGGCA GATTAAAGCA ATTATGAAAG AGGTCATTCC TTTTCTGAAG 
GAACACATGG ATGAAGTATG CTCTTCGCAG TTACTGACAT CAGTAAGACG TATGGTCTTG 
ACCCTTACAC AACAAAATGA TGAAAGTAAA GAGTTTGTGA AGTTCTTTCA TAAGCAGCTT 
GGCAGTATAC TTCAGGATTC ACTGGCAAAA TTTGCTGGTA GAAAACTGAA AGATTGTGGA 
GAGGATCTTC TAGTGGAGAT CTCTGAAGTG TTATTTAATG AATTAGCCTT TTTTAAACTC 
ATGCAAGACT TGGACAACAA CAGTATTTCT GTAAAGCAGA GATGTAAAAG AAAAATAGAA 
GCTACTGAAG TGATACAGTC GTATGCTAAA GAGGCAAAAA AAGGTCTCCA GGTGGATGTT 
TGTTCATCTG CTGAAGATGT CGATGAGGAC AAAGACAAGG ATGAGACTGA AACTGTTAAA 
CAAGTACAGG ACTCAGAAAT GTATGATGGG AATAGAGTTC CTGAAAGTAT TAGGTCTGAT 
GCATCTGATC AGGAGGAAGA TGATGAAAGT GAAAGCGGTC CAGTGGCAAT AAGTTTATCA 
AAAGCAGAAA CCCAAGCTCT GACTAACTAT GGTAGTGGAG AAGATGAGAA TGAAGATGAA 
GAAATAGAAT TTGAGGAAGG ACCTGTTGAT GTGCAGACGT CATTACAAGC CAGCAGTGAA 
ACAACAACTG AAAATGAACA GAATTCAAAC CAAGAACAAA GTAAGACAAA AAGCAGTGAG 
ATTCTGTCAT CAGAACAAGA ATCTGTTTAT GCTAAAGGTG AACAACATGT GGCTACAATT 
TTGCCTTATT ACCTCAATGT AATGGAGAAT GCACCGCCTT TAACGGTCAA TACCCCAGAA 
TCTTTTATAA CAGACAGTAT GAAAACAGAA GAATCAAGTT CATCTTTACC AGTAAATGAA 
ACTCAAACAC TGGATACAAC GTGTGTAGGA AACAAATCTG CTGCAAGTTC TGAAAGCTCC 
ATGGCTGGCA GCCCTGATAC AGAGTCACCT GTGTTAGTGA ATGAATATGA AGCTGGTTCT 
GGAAATGTAA GTCAAAGATC TGATGAAGAT GACTTTGTGA AAGTTGAAGA CTTGCCCCTT 
AAACTTGCAG TGTATTCAGA GGCAGATTTA ATGAAGAAAA TGGCAATGGA GGCCCAAACG 
AACAGTTTGT CTGATGAATT ACTGGGTGGA GGTGGAGCTC AAGATCAAGA ATTAGTAGGA 
GATGCCCAAA CTTTGAAAGA ACCTGAAACT TTTGGAGTTC AAAGTGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021141538.1
CDS	140..6370
Translation	MATGGGPFEEGMNDQDLPSWSNDSFDDRLNNTDWGSQQKKANRSSEKNKKKLGGEAETRLTNDISPESSPGIGRRKTRTPHSFPHARYMTQMSVPEQAELERLKQRINFSDLDQRSIGSDSQGRATAANNKRQLNENKKPFNFLSLQINTNKSKDPASCSQKKESGVSVQRKELFGAALSKDFLQNCQVSAQEDGGGEPAMDSSQIVSRLVQIRDYIAKASSMRDDLVEKNERSANVERLSHLIDHLKEQEKSYLKFLQKMLARQNEEDDVRTIDSAVGSGSVGESTSLNIDVQSEASDTTARDPQQEAKEELENLKKQHDLLKRMLKQQEQLKALQGRQAALLALQHKAEQAIAVMDDSVVTETTGSVSGVSLTSELNEELNDLIQRFHNQLHDSQTQSVPDNRRQAESLSLTREVSQSRNSSMSERLSDEKAQLFNKMRMLQGKKQKMDKLLGELHTLRDQHLNNSSFFPASGSPQRSIDQRSTTSAASGPAGIVTVVNGESNSLASAPYPPDSLVSQNESEEDDNLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDSVNENTKEEEETEESESDSEREDPQPVTNIRNPQGISSWSDINSNSNVQCGTNNRDGRHLNTDCEINNRSAANIRTLKMSSPLDCHNREDDKDIGLPQCEDEEVEEDRVSEDSASSHRSSLGDTAGDAEFEQKINRLIAAKQKLRQLQNLAAMVQDDDPAPQTTIANASNIGDLFLGEMEETKQQPNNVRASTNKLQKDVELNEKAREKFYEAKLQQQQQELKQLQEERRKLIEIQEKIQVLQKACPDLQLSAGLGDCTANRQTSPATSTPAMNDTAGKPSLEFDESVPVGNELWSEMRRHEILREELRQRRKQLEALMAEHQRRKELAETISTVAASVKSEGSEARRTPQQSRTENRTMATWGGSTQCALDEEDGDEDGYLSDGAGQAEEDDDDASSANDSFSVYPNNNVPENAYFVKENKDRWKNYRPLSADGNYRPLSKARQQQNISMRRQENLRWMSELSYVEEKERWQEQINQLKKQHEFSVSICQTLMQDQQTLSCLLQTLLTGPYSMMPNNVASSQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNDLVRQQEEQRYEEKSSRKDRGSGVPPPPSPVFCPFSFPPQPLNLFNVPGFTNFSSFAPDINYNPVFPSGFGDFAHNISPHSSEQQQQHPLDHNASGKTEYMAFPKPFESSSSNGAEKQRRSHRQPEEEMEKRSAWLNDSQDVKKDDQSQLKASFAVSVQNIASNHKNQSDMSRRREFDEESLESFSSMPDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKNKRKSSSQLKGRITNIDYESASASSVCEPCKSNKSRHSEEVGHAKVFSKRNREQLEKIIKYSRSTEMSSAHARRILQQSNRNACIEAPETGSDLSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHLSENNEKGKCTKSLNSAAWIASNSELTPSESLASTDDETFGKNFSTEACQDCEQNDADNGSSMSTSSNFEPFATDDLGNTVIHLDQALARMREYERMKIEAESTLDSEGCSSNFQGASAAKLEGGQSASECVPVPQSSEASAVPCPHIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSISVKQRCKRKIEATEVIQSYAKEAKKGLQVDVCSSAEDVDEDKDKDETETVKQVQDSEMYDGNRVPESIRSDASDQEEDDESESGPVAISLSKAETQALTNYGSGEDENEDEEIEFEEGPVDVQTSLQASSETTTENEQNSNQEQSKTKSSEILSSEQESVYAKGEQHVATILPYYLNVMENAPPLTVNTPESFITDSMKTEESSSSLPVNETQTLDTTCVGNKSAASSESSMAGSPDTESPVLVNEYEAGSGNVSQRSDEDDFVKVEDLPLKLAVYSEADLMKKMAMEAQTNSLSDELLGGGGAQDQELVGDAQTLKEPETFGVQSA

Target ORF information:

RefSeq Version	XM_021285863.1
Organism	Columba livia(rock pigeon)
Definition	Columba livia pericentriolar material 1 (PCM1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021285863.1

ORF Insert Sequence:

ATGGCAACAG GAGGTGGTCC CTTTGAAGAA GGCATGAATG ATCAAGACTT GCCTAGCTGG 
AGCAATGACA GCTTTGATGA CCGGCTGAAC AACACGGACT GGGGAAGTCA ACAGAAGAAA 
GCAAACAGAT CTTCAGAAAA AAACAAGAAA AAGCTTGGTG GGGAAGCTGA AACAAGGCTT 
ACTAATGATA TATCTCCAGA ATCCTCACCT GGAATAGGAC GACGAAAGAC CAGAACTCCT 
CATAGTTTTC CTCATGCTCG ATACATGACC CAGATGTCTG TTCCGGAGCA GGCTGAGCTA 
GAAAGGCTTA AACAAAGAAT AAACTTCAGT GATCTGGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGCA GGGCAACAGC TGCTAACAAC AAACGTCAAC TTAATGAAAA CAAAAAACCA 
TTCAACTTCC TGTCACTGCA GATAAACACT AACAAGAGCA AAGATCCTGC CTCATGTTCC 
CAAAAAAAGG AAAGTGGGGT GTCAGTCCAA CGTAAAGAGT TGTTTGGTGC TGCTCTGAGC 
AAGGATTTCT TGCAAAATTG TCAAGTCTCT GCTCAAGAAG ATGGAGGGGG AGAACCAGCT 
ATGGATAGTA GCCAGATTGT GAGCAGACTA GTTCAAATTC GCGACTATAT TGCTAAGGCC 
AGCTCCATGC GGGATGATCT TGTAGAGAAA AATGAAAGAT CGGCCAATGT TGAGCGTTTA 
TCACACCTTA TAGATCACCT TAAAGAGCAG GAGAAATCCT ATCTGAAATT TTTGCAAAAG 
ATGCTTGCTA GACAAAATGA GGAGGATGAT GTTCGGACTA TAGATTCAGC TGTGGGATCT 
GGTTCTGTAG GTGAGAGCAC ATCGCTAAAC ATTGATGTGC AGTCTGAGGC TTCAGATACC 
ACGGCTAGAG ATCCTCAACA GGAGGCTAAA GAGGAGTTGG AGAACTTGAA GAAGCAGCAT 
GATTTATTGA AAAGGATGCT AAAACAGCAA GAGCAATTAA AGGCTCTTCA AGGAAGACAG 
GCAGCTCTTC TTGCTTTGCA GCATAAAGCA GAGCAAGCAA TTGCTGTCAT GGATGATTCT 
GTTGTAACAG AGACTACAGG TAGTGTTTCA GGAGTAAGCC TTACATCAGA ACTGAATGAA 
GAATTGAATG ACTTAATTCA GCGCTTTCAC AACCAACTTC ATGATTCTCA GACACAGTCT 
GTACCAGACA ATAGAAGGCA AGCAGAAAGT CTTTCACTTA CTAGAGAGGT TTCACAAAGC 
AGAAACTCTT CAATGTCTGA ACGCCTGTCA GATGAGAAGG CGCAGCTTTT TAACAAGATG 
CGAATGTTGC AGGGTAAAAA GCAAAAAATG GACAAACTGT TAGGAGAACT TCATACACTT 
CGTGACCAAC ATCTAAATAA CTCCTCCTTT TTTCCTGCTT CAGGTTCTCC TCAAAGAAGT 
ATTGATCAAA GAAGTACAAC TTCAGCTGCT TCTGGTCCTG CAGGCATAGT AACTGTTGTC 
AATGGCGAAT CAAATAGCCT GGCATCTGCT CCCTATCCTC CTGATTCCCT GGTTTCTCAG 
AATGAGAGTG AAGAAGATGA CAATCTAAAT CCAACAGAAA AGCTTCAGAA ACTAAATGAA 
GTTCGTAAGA GGCTGAATGA GTTACGTGAG CTAGTTCACT ACTATGAGCA AACATCTGAT 
ATGATGACAG ATTCTGTGAA TGAAAACACT AAGGAAGAAG AAGAAACCGA AGAATCAGAA 
AGTGATTCTG AACGTGAGGA TCCACAGCCT GTTACAAATA TTAGAAATCC TCAAGGAATC 
AGTAGTTGGA GTGACATAAA TAGCAACTCA AATGTACAGT GTGGAACTAA TAACAGAGAT 
GGAAGACATC TTAATACAGA CTGTGAAATA AATAACAGAT CTGCTGCTAA CATAAGGACT 
CTAAAAATGT CGTCACCTTT AGACTGTCAT AATAGGGAGG ATGACAAAGA TATTGGCCTA 
CCCCAATGTG AAGATGAAGA AGTGGAAGAA GATAGAGTTA GTGAAGATTC TGCATCTAGT 
CACAGAAGCA GCCTGGGTGA TACTGCTGGA GATGCTGAGT TTGAGCAGAA GATCAATAGG 
CTTATAGCTG CAAAACAGAA ACTTAGACAA TTACAAAACC TTGCTGCTAT GGTGCAGGAT 
GATGATCCAG CACCTCAGAC AACAATTGCA AATGCATCTA ATATTGGTGA CTTGTTTTTG 
GGTGAGATGG AAGAGACAAA GCAGCAGCCA AACAATGTCC GAGCTAGTAC CAACAAATTA 
CAAAAAGATG TAGAACTGAA TGAAAAAGCA AGAGAGAAGT TTTATGAAGC TAAACTCCAG 
CAGCAGCAAC AGGAGCTTAA GCAGTTACAA GAAGAAAGAA GAAAACTGAT TGAAATTCAA 
GAAAAAATTC AGGTGTTACA GAAAGCTTGT CCTGATCTTC AATTGTCAGC TGGCTTGGGT 
GACTGCACAG CAAATAGACA GACTTCACCA GCAACATCAA CTCCAGCCAT GAATGACACA 
GCTGGCAAGC CTTCACTTGA ATTTGATGAA TCTGTACCAG TAGGCAATGA GTTATGGTCT 
GAGATGAGAA GACATGAGAT TTTAAGAGAA GAATTGCGAC AAAGAAGAAA GCAACTGGAA 
GCTCTAATGG CTGAGCATCA GAGGAGGAAA GAGCTCGCAG AAACAATATC TACTGTTGCT 
GCGTCTGTTA AAAGTGAAGG ATCAGAAGCT CGGCGTACTC CACAGCAGAG TAGGACTGAA 
AATAGGACAA TGGCTACCTG GGGAGGTTCT ACGCAGTGTG CACTAGATGA AGAGGATGGA 
GATGAAGATG GTTATCTCTC TGATGGAGCT GGTCAGGCAG AAGAAGATGA TGATGATGCG 
TCAAGTGCGA ATGACAGCTT CTCTGTTTAT CCCAATAACA ACGTACCAGA AAATGCATAT 
TTTGTTAAAG AAAACAAGGA TAGGTGGAAA AATTACCGTC CTCTTTCAGC AGATGGAAAT 
TATCGTCCAT TGTCTAAGGC CAGACAACAG CAAAACATAA GTATGCGACG TCAGGAAAAC 
CTTCGGTGGA TGTCTGAACT TTCATATGTG GAAGAAAAGG AACGATGGCA AGAGCAGATC 
AATCAGTTGA AGAAACAGCA TGAATTTAGT GTCAGCATTT GTCAAACTTT AATGCAGGAT 
CAGCAGACCC TCTCTTGTCT TCTACAGACT TTGCTTACAG GCCCTTACAG TATGATGCCC 
AATAATGTTG CATCTTCACA AGTACATCTT ATTATGCATC AGCTAAACCA GTGTTACACT 
CAACTGACAT GGCAGCAGAA TAACGTCCAG AGGTTGAAAC AAATGTTAAA TGATCTTGTG 
CGTCAGCAAG AAGAACAACG GTATGAAGAG AAGTCATCAA GAAAGGATAG AGGCAGTGGT 
GTACCCCCAC CTCCATCTCC TGTTTTCTGT CCGTTCAGCT TTCCTCCACA ACCTTTGAAC 
CTGTTTAATG TACCAGGATT TACTAATTTT TCTTCCTTTG CTCCAGATAT TAACTATAAT 
CCAGTGTTTC CTTCTGGTTT TGGAGATTTT GCACACAATA TTTCTCCACA CAGCAGTGAG 
CAGCAGCAGC AACATCCTCT AGATCATAAT GCATCTGGGA AAACTGAGTA TATGGCGTTC 
CCCAAACCTT TTGAAAGCAG TTCCTCTAAT GGAGCGGAAA AACAAAGGAG GAGTCACAGA 
CAACCTGAAG AGGAAATGGA AAAAAGATCA GCTTGGCTTA ATGATAGCCA GGACGTGAAA 
AAAGATGATC AGTCTCAGCT GAAAGCAAGT TTTGCTGTTT CAGTACAAAA CATTGCTTCC 
AATCATAAAA ATCAGTCTGA TATGAGCAGA AGAAGAGAGT TTGATGAAGA GTCTTTGGAG 
AGTTTTAGTA GCATGCCTGA TCCAGTAGAC CCCACTACTG TGACAAAGAC ATTTAAATCT 
AGAAAAGCAT CAGCACAAGC AAGTTTGGCA TCAAAAGATA AAACACCTAA ATCGAAGAAT 
AAGAGGAAGA GTTCTTCTCA GCTAAAAGGC AGAATTACAA ATATTGATTA TGAAAGTGCC 
AGTGCTTCTA GTGTGTGTGA ACCCTGCAAG AGCAATAAAA GCAGACACTC TGAAGAGGTT 
GGTCATGCAA AGGTGTTCAG CAAAAGGAAT CGGGAACAAC TGGAAAAAAT CATTAAATAC 
AGTAGATCTA CAGAAATGTC TTCAGCGCAT GCTAGGAGAA TTCTGCAGCA GTCTAACAGA 
AATGCATGCA TTGAAGCGCC AGAAACTGGT AGTGATCTTT CTATGTTTGA AGCTTTACGA 
GACACAATTT ATTCTGAAGT GGCAACTCTT ATTTCTCAAA ATGAGTCTCG TCCCCACTTT 
CTTATTGAAC TTTTCCATGA GCTTCAGCTG CTAAATACAG ATTACCTGAG GCAAAGAGCT 
CTATACGCTC TACAGGATAT AGTGACCAGA CATTTATCTG AGAACAATGA AAAAGGGAAG 
TGCACAAAAT CACTGAATTC TGCAGCATGG ATAGCATCAA ATTCTGAACT CACTCCCAGT 
GAAAGCCTTG CTTCTACAGA TGATGAAACT TTTGGCAAAA ACTTTTCTAC AGAAGCATGT 
CAAGATTGTG AACAAAATGA TGCAGACAAT GGGAGTAGCA TGTCTACATC TTCAAATTTT 
GAACCCTTTG CCACTGATGA CCTTGGCAAC ACAGTGATTC ACTTAGATCA AGCTTTGGCT 
AGGATGAGGG AGTATGAGCG TATGAAAATT GAAGCTGAAA GTACCCTTGA CTCTGAGGGC 
TGCTCTAGTA ATTTTCAGGG TGCTTCTGCT GCTAAATTAG AAGGAGGTCA AAGTGCCAGT 
GAATGTGTTC CTGTGCCACA GTCAAGTGAA GCTTCTGCTG TTCCATGTCC TCATATAGAC 
ACTCAGCAGC TTGACCGGCA GATTAAAGCA ATTATGAAAG AGGTCATTCC TTTTCTGAAG 
GAACACATGG ATGAAGTATG CTCTTCGCAG TTACTGACAT CAGTAAGACG TATGGTCTTG 
ACCCTTACAC AACAAAATGA TGAAAGTAAA GAGTTTGTGA AGTTCTTTCA TAAGCAGCTT 
GGCAGTATAC TTCAGGATTC ACTGGCAAAA TTTGCTGGTA GAAAACTGAA AGATTGTGGA 
GAGGATCTTC TAGTGGAGAT CTCTGAAGTG TTATTTAATG AATTAGCCTT TTTTAAACTC 
ATGCAAGACT TGGACAACAA CAGTATTTCT GTAAAGCAGA GATGTAAAAG AAAAATAGAA 
GCTACTGAAG TGATACAGTC GTATGCTAAA GAGGCAAAAA AAGGTCTCCA GGTGGATGTT 
TGTTCATCTG CTGAAGATGT CGATGAGGAC AAAGACAAGG ATGAGACTGA AACTGTTAAA 
CAAGTACAGG ACTCAGAAAT GTATGATGGG AATAGAGTTC CTGAAAGTAT TAGGTCTGAT 
GCATCTGATC AGGAGGAAGA TGATGAAAGT GAAAGCGGTC CAGTGGCAAT AAGTTTATCA 
AAAGCAGAAA CCCAAGCTCT GACTAACTAT GGTAGTGGAG AAGATGAGAA TGAAGATGAA 
GAAATAGAAT TTGAGGAAGG ACCTGTTGAT GTGCAGACGT CATTACAAGC CAGCAGTGAA 
ACAACAACTG AAAATGAACA GAATTCAAAC CAAGAACAAA GTAAGACAAA AAGCAGTGAG 
ATTCTGTCAT CAGAACAAGA ATCTGTTTAT GCTAAAGGTG AACAACATGT GGCTACAATT 
TTGCCTTATT ACCTCAATGT AATGGAGAAT GCACCGCCTT TAACGGTCAA TACCCCAGAA 
TCTTTTATAA CAGACAGTAT GAAAACAGAA GAATCAAGTT CATCTTTACC AGTAAATGAA 
ACTCAAACAC TGGATACAAC GTGTGTAGGA AACAAATCTG CTGCAAGTTC TGAAAGCTCC 
ATGGCTGGCA GCCCTGATAC AGAGTCACCT GTGTTAGTGA ATGAATATGA AGCTGGTTCT 
GGAAATGTAA GTCAAAGATC TGATGAAGAT GACTTTGTGA AAGTTGAAGA CTTGCCCCTT 
AAACTTGCAG TGTATTCAGA GGCAGATTTA ATGAAGAAAA TGGCAATGGA GGCCCAAACG 
AACAGTTTGT CTGATGAATT ACTGGGTGGA GGTGGAGCTC AAGATCAAGA ATTAGTAGGA 
GATGCCCAAA CTTTGAAAGA ACCTGAAACT TTTGGAGTTC AAAGTGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_021285864.1 ) cDNA ORF clone, Columba livia(rock pigeon) -> XP_021141539.1 Columba livia pericentriolar material 1 (PCM1), transcript variant X11, mRNA.

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CloneID	OCo293167
Clone ID Related Accession (Same CDS sequence)	XM_021285864.1
Accession Version	XM_021285864.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-23
Organism	Columba livia(rock pigeon)
Product	pericentriolar material 1 protein isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004973253.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Columba livia Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACAG GAGGTGGTCC CTTTGAAGAA GGCATGAATG ATCAAGACTT GCCTAGCTGG 
AGCAATGACA GCTTTGATGA CCGGCTGAAC AACACGGACT GGGGAAGTCA ACAGAAGAAA 
GCAAACAGAT CTTCAGAAAA AAACAAGAAA AAGCTTGGTG GGGAAGCTGA AACAAGGCTT 
ACTAATGATA TATCTCCAGA ATCCTCACCT GGAATAGGAC GACGAAAGAC CAGAACTCCT 
CATAGTTTTC CTCATGCTCG ATACATGACC CAGATGTCTG TTCCGGAGCA GGCTGAGCTA 
GAAAGGCTTA AACAAAGAAT AAACTTCAGT GATCTGGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGCA GGGCAACAGC TGCTAACAAC AAACGTCAAC TTAATGAAAA CAAAAAACCA 
TTCAACTTCC TGTCACTGCA GATAAACACT AACAAGAGCA AAGATCCTGC CTCATGTTCC 
CAAAAAAAGG AAAGTGGGGT GTCAGTCCAA CGTAAAGAGT TGTTTGGTGC TGCTCTGAGC 
AAGGATTTCT TGCAAAATTG TCAAGTCTCT GCTCAAGAAG ATGGAGGGGG AGAACCAGCT 
ATGGATAGTA GCCAGATTGT GAGCAGACTA GTTCAAATTC GCGACTATAT TGCTAAGGCC 
AGCTCCATGC GGGATGATCT TGTAGAGAAA AATGAAAGAT CGGCCAATGT TGAGCGTTTA 
TCACACCTTA TAGATCACCT TAAAGAGCAG GAGAAATCCT ATCTGAAATT TTTGCAAAAG 
ATGCTTGCTA GACAAAATGA GGAGGATGAT GTTCGGACTA TAGATTCAGC TGTGGGATCT 
GGTTCTGTAG GTGAGAGCAC ATCGCTAAAC ATTGATGTGC AGTCTGAGGC TTCAGATACC 
ACGGAGGTAT CTTTTAGTTT GAGCATTCGG CCCTGCATTG AGGACAAACT AGGGAATTCA 
GCTTCACAAG AACAGGTTAC AGACTTTGAT GTTACTGCAA GCCCTAAAGG GAAAAATGAC 
AGAGCTGCCC TGAATGACAG GGAAATCTGG CCTTGTAGGA TTAATAGCCG AGAACATGGA 
TTGCCTTCAA AGGCTAGAGA TCCTCAACAG GAGGCTAAAG AGGAGTTGGA GAACTTGAAG 
AAGCAGCATG ATTTATTGAA AAGGATGCTA AAACAGCAAG AGCAATTAAA GGCTCTTCAA 
GGAAGACAGG CAGCTCTTCT TGCTTTGCAG CATAAAGCAG AGCAAGCAAT TGCTGTCATG 
GATGATTCTG TTGTAACAGA GACTACAGGT AGTGTTTCAG GAGTAAGCCT TACATCAGAA 
CTGAATGAAG AATTGAATGA CTTAATTCAG CGCTTTCACA ACCAACTTCA TGATTCTCAG 
ACACAGTCTG TACCAGACAA TAGAAGGCAA GCAGAAAGTC TTTCACTTAC TAGAGAGGTT 
TCACAAAGCA GAAACTCTTC AATGTCTGAA CGCCTGTCAG ATGAGAAGGC GCAGCTTTTT 
AACAAGATGC GAATGTTGCA GGGTAAAAAG CAAAAAATGG ACAAACTGTT AGGAGAACTT 
CATACACTTC GTGACCAACA TCTAAATAAC TCCTCCTTTT TTCCTGCTTC AGGTTCTCCT 
CAAAGAAGTA TTGATCAAAG AAGTACAACT TCAGCTGCTT CTGGTCCTGC AGGCATAGTA 
ACTGTTGTCA ATGGCGAATC AAATAGCCTG GCATCTGCTC CCTATCCTCC TGATTCCCTG 
GTTTCTCAGA ATGAGAGTGA AGAAGATGAC AATCTAAATC CAACAGAAAA GCTTCAGAAA 
CTAAATGAAG TTCGTAAGAG GCTGAATGAG TTACGTGAGC TAGTTCACTA CTATGAGCAA 
ACATCTGATA TGATGACAGA TTCTGTGAAT GAAAACACTA AGGAAGAAGA AGAAACCGAA 
GAATCAGAAA GTGATTCTGA ACGTGAGGAT CCACAGCCTG TTACAAATAT TAGAAATCCT 
CAAGGAATCA GTAGTTGGAG TGACATAAAT AGCAACTCAA ATGTACAGTG TGGAACTAAT 
AACAGAGATG GAAGACATCT TAATACAGAC TGTGAAATAA ATAACAGATC TGCTGCTAAC 
ATAAGGACTC TAAAAATGTC GTCACCTTTA GACTGTCATA ATAGGGAGGA TGACAAAGAT 
ATTGGCCTAC CCCAATGTGA AGATGAAGAA GTGGAAGAAG ATAGAGTTAG TGAAGATTCT 
GCATCTAGTC ACAGAAGCAG CCTGGGTGAT ACTGCTGGAG ATGCTGAGTT TGAGCAGAAG 
ATCAATAGGC TTATAGCTGC AAAACAGAAA CTTAGACAAT TACAAAACCT TGCTGCTATG 
GTGCAGGATG ATGATCCAGC ACCTCAGACA ACAATTGCAA ATGCATCTAA TATTGGTGAC 
TTGTTTTTGG GTGAGATGGA AGAGACAAAG CAGCAGCCAA ACAATGTCCG AGCTAGTACC 
AACAAATTAC AAAAAGATGT AGAACTGAAT GAAAAAGCAA GAGAGAAGTT TTATGAAGCT 
AAACTCCAGC AGCAGCAACA GGAGCTTAAG CAGTTACAAG AAGAAAGAAG AAAACTGATT 
GAAATTCAAG AAAAAATTCA GGTGTTACAG AAAGCTTGTC CTGATCTTCA ATTGTCAGCT 
GGCTTGGGTG ACTGCACAGC AAATAGACAG ACTTCACCAG CAACATCAAC TCCAGCCATG 
AATGACACAG CTGGCAAGCC TTCACTTGAA TTTGATGAAT CTGTACCAGT AGGCAATGAG 
TTATGGTCTG AGATGAGAAG ACATGAGATT TTAAGAGAAG AATTGCGACA AAGAAGAAAG 
CAACTGGAAG CTCTAATGGC TGAGCATCAG AGGAGGAAAG AGCTCGCAGA AACAATATCT 
ACTGTTGCTG CGTCTGTTAA AAGTGAAGGA TCAGAAGCTC GGCGTACTCC ACAGCAGAGT 
AGGACTGAAA AGTGGAAAAA TTACCGTCCT CTTTCAGCAG ATGGAAATTA TCGTCCATTG 
TCTAAGGCCA GACAACAGCA AAACATAAGT ATGCGACGTC AGGAAAACCT TCGGTGGATG 
TCTGAACTTT CATATGTGGA AGAAAAGGAA CGATGGCAAG AGCAGATCAA TCAGTTGAAG 
AAACAGCATG AATTTAGTGT CAGCATTTGT CAAACTTTAA TGCAGGATCA GCAGACCCTC 
TCTTGTCTTC TACAGACTTT GCTTACAGGC CCTTACAGTA TGATGCCCAA TAATGTTGCA 
TCTTCACAAG TACATCTTAT TATGCATCAG CTAAACCAGT GTTACACTCA ACTGACATGG 
CAGCAGAATA ACGTCCAGAG GTTGAAACAA ATGTTAAATG ATCTTGTGCG TCAGCAAGAA 
GAACAACGGT ATGAAGAGAA GTCATCAAGA AAGGATAGAG GCAGTGGTGT ACCCCCACCT 
CCATCTCCTG TTTTCTGTCC GTTCAGCTTT CCTCCACAAC CTTTGAACCT GTTTAATGTA 
CCAGGATTTA CTAATTTTTC TTCCTTTGCT CCAGATATTA ACTATAATCC AGTGTTTCCT 
TCTGGTTTTG GAGATTTTGC ACACAATATT TCTCCACACA GCAGTGAGCA GCAGCAGCAA 
CATCCTCTAG ATCATAATGC ATCTGGGAAA ACTGAGTATA TGGCGTTCCC CAAACCTTTT 
GAAAGCAGTT CCTCTAATGG AGCGGAAAAA CAAAGGAGGA GTCACAGACA ACCTGAAGAG 
GAAATGGAAA AAAGATCAGC TTGGCTTAAT GATAGCCAGG ACGTGAAAAA AGATGATCAG 
TCTCAGCTGA AAGCAAGTTT TGCTGTTTCA GTACAAAACA TTGCTTCCAA TCATAAAAAT 
CAGTCTGATA TGAGCAGAAG AAGAGAGTTT GATGAAGAGT CTTTGGAGAG TTTTAGTAGC 
ATGCCTGATC CAGTAGACCC CACTACTGTG ACAAAGACAT TTAAATCTAG AAAAGCATCA 
GCACAAGCAA GTTTGGCATC AAAAGATAAA ACACCTAAAT CGAAGAATAA GAGGAAGAGT 
TCTTCTCAGC TAAAAGGCAG AATTACAAAT ATTGATTATG AAAGTGCCAG TGCTTCTAGT 
GTGTGTGAAC CCTGCAAGAG CAATAAAAGC AGACACTCTG AAGAGGTTGG TCATGCAAAG 
GTGTTCAGCA AAAGGAATCG GGAACAACTG GAAAAAATCA TTAAATACAG TAGATCTACA 
GAAATGTCTT CAGCGCATGC TAGGAGAATT CTGCAGCAGT CTAACAGAAA TGCATGCATT 
GAAGCGCCAG AAACTGGTAG TGATCTTTCT ATGTTTGAAG CTTTACGAGA CACAATTTAT 
TCTGAAGTGG CAACTCTTAT TTCTCAAAAT GAGTCTCGTC CCCACTTTCT TATTGAACTT 
TTCCATGAGC TTCAGCTGCT AAATACAGAT TACCTGAGGC AAAGAGCTCT ATACGCTCTA 
CAGGATATAG TGACCAGACA TTTATCTGAG AACAATGAAA AAGGGAAGTG CACAAAATCA 
CTGAATTCTG CAGCATGGAT AGCATCAAAT TCTGAACTCA CTCCCAGTGA AAGCCTTGCT 
TCTACAGATG ATGAAACTTT TGGCAAAAAC TTTTCTACAG AAGCATGTCA AGATTGTGAA 
CAAAATGATG CAGACAATGG GAGTAGCATG TCTACATCTT CAAATTTTGA ACCCTTTGCC 
ACTGATGACC TTGGCAACAC AGTGATTCAC TTAGATCAAG CTTTGGCTAG GATGAGGGAG 
TATGAGCGTA TGAAAATTGA AGCTGAAAGT ACCCTTGACT CTGAGGGCTG CTCTAGTAAT 
TTTCAGGGTG CTTCTGCTGC TAAATTAGAA GGAGGTCAAA GTGCCAGTGA ATGTGTTCCT 
GTGCCACAGT CAAGTGAAGC TTCTGCTGTT CCATGTCCTC ATATAGACAC TCAGCAGCTT 
GACCGGCAGA TTAAAGCAAT TATGAAAGAG GTCATTCCTT TTCTGAAGGA ACACATGGAT 
GAAGTATGCT CTTCGCAGTT ACTGACATCA GTAAGACGTA TGGTCTTGAC CCTTACACAA 
CAAAATGATG AAAGTAAAGA GTTTGTGAAG TTCTTTCATA AGCAGCTTGG CAGTATACTT 
CAGGATTCAC TGGCAAAATT TGCTGGTAGA AAACTGAAAG ATTGTGGAGA GGATCTTCTA 
GTGGAGATCT CTGAAGTGTT ATTTAATGAA TTAGCCTTTT TTAAACTCAT GCAAGACTTG 
GACAACAACA GTATTTCTGT AAAGCAGAGA TGTAAAAGAA AAATAGAAGC TACTGAAGTG 
ATACAGTCGT ATGCTAAAGA GGCAAAAAAA GGTCTCCAGG TGGATGTTTG TTCATCTGCT 
GAAGATGTCG ATGAGGACAA AGACAAGGAT GAGACTGAAA CTGTTAAACA AGTACAGGAC 
TCAGAAATGT ATGATGGGAA TAGAGTTCCT GAAAGTATTA GGTCTGATGC ATCTGATCAG 
GAGGAAGATG ATGAAAGTGA AAGCGGTCCA GTGGCAATAA GTTTATCAAA AGCAGAAACC 
CAAGCTCTGA CTAACTATGG TAGTGGAGAA GATGAGAATG AAGATGAAGA AATAGAATTT 
GAGGAAGGAC CTGTTGATGT GCAGACGTCA TTACAAGCCA GCAGTGAAAC AACAACTGAA 
AATGAACAGA ATTCAAACCA AGAACAAAGT AAGACAAAAA GCAGTGAGAT TCTGTCATCA 
GAACAAGAAT CTGTTTATGC TAAAGGTGAA CAACATGTGG CTACAATTTT GCCTTATTAC 
CTCAATGTAA TGGAGAATGC ACCGCCTTTA ACGGTCAATA CCCCAGAATC TTTTATAACA 
GACAGTATGA AAACAGAAGA ATCAAGTTCA TCTTTACCAG TAAATGAAAC TCAAACACTG 
GATACAACGT GTGTAGGAAA CAAATCTGCT GCAAGTTCTG AAAGCTCCAT GGCTGGCAGC 
CCTGATACAG AGTCACCTGT GTTAGTGAAT GAATATGAAG CTGGTTCTGG AAATGTAAGT 
CAAAGATCTG ATGAAGATGA CTTTGTGAAA GTTGAAGACT TGCCCCTTAA ACTTGCAGTG 
TATTCAGAGG CAGATTTAAT GAAGAAAATG GCAATGGAGG CCCAAACGAA CAGTTTGTCT 
GATGAATTAC TGGGTGGAGG TGGAGCTCAA GATCAAGAAT TAGTAGGAGA TGCCCAAACT 
TTGAAAGAAC CTGAAACTTT TGGAGTTCAA AGTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021141539.1
CDS	140..6358
Translation	MATGGGPFEEGMNDQDLPSWSNDSFDDRLNNTDWGSQQKKANRSSEKNKKKLGGEAETRLTNDISPESSPGIGRRKTRTPHSFPHARYMTQMSVPEQAELERLKQRINFSDLDQRSIGSDSQGRATAANNKRQLNENKKPFNFLSLQINTNKSKDPASCSQKKESGVSVQRKELFGAALSKDFLQNCQVSAQEDGGGEPAMDSSQIVSRLVQIRDYIAKASSMRDDLVEKNERSANVERLSHLIDHLKEQEKSYLKFLQKMLARQNEEDDVRTIDSAVGSGSVGESTSLNIDVQSEASDTTEVSFSLSIRPCIEDKLGNSASQEQVTDFDVTASPKGKNDRAALNDREIWPCRINSREHGLPSKARDPQQEAKEELENLKKQHDLLKRMLKQQEQLKALQGRQAALLALQHKAEQAIAVMDDSVVTETTGSVSGVSLTSELNEELNDLIQRFHNQLHDSQTQSVPDNRRQAESLSLTREVSQSRNSSMSERLSDEKAQLFNKMRMLQGKKQKMDKLLGELHTLRDQHLNNSSFFPASGSPQRSIDQRSTTSAASGPAGIVTVVNGESNSLASAPYPPDSLVSQNESEEDDNLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDSVNENTKEEEETEESESDSEREDPQPVTNIRNPQGISSWSDINSNSNVQCGTNNRDGRHLNTDCEINNRSAANIRTLKMSSPLDCHNREDDKDIGLPQCEDEEVEEDRVSEDSASSHRSSLGDTAGDAEFEQKINRLIAAKQKLRQLQNLAAMVQDDDPAPQTTIANASNIGDLFLGEMEETKQQPNNVRASTNKLQKDVELNEKAREKFYEAKLQQQQQELKQLQEERRKLIEIQEKIQVLQKACPDLQLSAGLGDCTANRQTSPATSTPAMNDTAGKPSLEFDESVPVGNELWSEMRRHEILREELRQRRKQLEALMAEHQRRKELAETISTVAASVKSEGSEARRTPQQSRTEKWKNYRPLSADGNYRPLSKARQQQNISMRRQENLRWMSELSYVEEKERWQEQINQLKKQHEFSVSICQTLMQDQQTLSCLLQTLLTGPYSMMPNNVASSQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNDLVRQQEEQRYEEKSSRKDRGSGVPPPPSPVFCPFSFPPQPLNLFNVPGFTNFSSFAPDINYNPVFPSGFGDFAHNISPHSSEQQQQHPLDHNASGKTEYMAFPKPFESSSSNGAEKQRRSHRQPEEEMEKRSAWLNDSQDVKKDDQSQLKASFAVSVQNIASNHKNQSDMSRRREFDEESLESFSSMPDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKNKRKSSSQLKGRITNIDYESASASSVCEPCKSNKSRHSEEVGHAKVFSKRNREQLEKIIKYSRSTEMSSAHARRILQQSNRNACIEAPETGSDLSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHLSENNEKGKCTKSLNSAAWIASNSELTPSESLASTDDETFGKNFSTEACQDCEQNDADNGSSMSTSSNFEPFATDDLGNTVIHLDQALARMREYERMKIEAESTLDSEGCSSNFQGASAAKLEGGQSASECVPVPQSSEASAVPCPHIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSISVKQRCKRKIEATEVIQSYAKEAKKGLQVDVCSSAEDVDEDKDKDETETVKQVQDSEMYDGNRVPESIRSDASDQEEDDESESGPVAISLSKAETQALTNYGSGEDENEDEEIEFEEGPVDVQTSLQASSETTTENEQNSNQEQSKTKSSEILSSEQESVYAKGEQHVATILPYYLNVMENAPPLTVNTPESFITDSMKTEESSSSLPVNETQTLDTTCVGNKSAASSESSMAGSPDTESPVLVNEYEAGSGNVSQRSDEDDFVKVEDLPLKLAVYSEADLMKKMAMEAQTNSLSDELLGGGGAQDQELVGDAQTLKEPETFGVQSA

Target ORF information:

RefSeq Version	XM_021285864.1
Organism	Columba livia(rock pigeon)
Definition	Columba livia pericentriolar material 1 (PCM1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021285864.1

ORF Insert Sequence:

ATGGCAACAG GAGGTGGTCC CTTTGAAGAA GGCATGAATG ATCAAGACTT GCCTAGCTGG 
AGCAATGACA GCTTTGATGA CCGGCTGAAC AACACGGACT GGGGAAGTCA ACAGAAGAAA 
GCAAACAGAT CTTCAGAAAA AAACAAGAAA AAGCTTGGTG GGGAAGCTGA AACAAGGCTT 
ACTAATGATA TATCTCCAGA ATCCTCACCT GGAATAGGAC GACGAAAGAC CAGAACTCCT 
CATAGTTTTC CTCATGCTCG ATACATGACC CAGATGTCTG TTCCGGAGCA GGCTGAGCTA 
GAAAGGCTTA AACAAAGAAT AAACTTCAGT GATCTGGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGCA GGGCAACAGC TGCTAACAAC AAACGTCAAC TTAATGAAAA CAAAAAACCA 
TTCAACTTCC TGTCACTGCA GATAAACACT AACAAGAGCA AAGATCCTGC CTCATGTTCC 
CAAAAAAAGG AAAGTGGGGT GTCAGTCCAA CGTAAAGAGT TGTTTGGTGC TGCTCTGAGC 
AAGGATTTCT TGCAAAATTG TCAAGTCTCT GCTCAAGAAG ATGGAGGGGG AGAACCAGCT 
ATGGATAGTA GCCAGATTGT GAGCAGACTA GTTCAAATTC GCGACTATAT TGCTAAGGCC 
AGCTCCATGC GGGATGATCT TGTAGAGAAA AATGAAAGAT CGGCCAATGT TGAGCGTTTA 
TCACACCTTA TAGATCACCT TAAAGAGCAG GAGAAATCCT ATCTGAAATT TTTGCAAAAG 
ATGCTTGCTA GACAAAATGA GGAGGATGAT GTTCGGACTA TAGATTCAGC TGTGGGATCT 
GGTTCTGTAG GTGAGAGCAC ATCGCTAAAC ATTGATGTGC AGTCTGAGGC TTCAGATACC 
ACGGAGGTAT CTTTTAGTTT GAGCATTCGG CCCTGCATTG AGGACAAACT AGGGAATTCA 
GCTTCACAAG AACAGGTTAC AGACTTTGAT GTTACTGCAA GCCCTAAAGG GAAAAATGAC 
AGAGCTGCCC TGAATGACAG GGAAATCTGG CCTTGTAGGA TTAATAGCCG AGAACATGGA 
TTGCCTTCAA AGGCTAGAGA TCCTCAACAG GAGGCTAAAG AGGAGTTGGA GAACTTGAAG 
AAGCAGCATG ATTTATTGAA AAGGATGCTA AAACAGCAAG AGCAATTAAA GGCTCTTCAA 
GGAAGACAGG CAGCTCTTCT TGCTTTGCAG CATAAAGCAG AGCAAGCAAT TGCTGTCATG 
GATGATTCTG TTGTAACAGA GACTACAGGT AGTGTTTCAG GAGTAAGCCT TACATCAGAA 
CTGAATGAAG AATTGAATGA CTTAATTCAG CGCTTTCACA ACCAACTTCA TGATTCTCAG 
ACACAGTCTG TACCAGACAA TAGAAGGCAA GCAGAAAGTC TTTCACTTAC TAGAGAGGTT 
TCACAAAGCA GAAACTCTTC AATGTCTGAA CGCCTGTCAG ATGAGAAGGC GCAGCTTTTT 
AACAAGATGC GAATGTTGCA GGGTAAAAAG CAAAAAATGG ACAAACTGTT AGGAGAACTT 
CATACACTTC GTGACCAACA TCTAAATAAC TCCTCCTTTT TTCCTGCTTC AGGTTCTCCT 
CAAAGAAGTA TTGATCAAAG AAGTACAACT TCAGCTGCTT CTGGTCCTGC AGGCATAGTA 
ACTGTTGTCA ATGGCGAATC AAATAGCCTG GCATCTGCTC CCTATCCTCC TGATTCCCTG 
GTTTCTCAGA ATGAGAGTGA AGAAGATGAC AATCTAAATC CAACAGAAAA GCTTCAGAAA 
CTAAATGAAG TTCGTAAGAG GCTGAATGAG TTACGTGAGC TAGTTCACTA CTATGAGCAA 
ACATCTGATA TGATGACAGA TTCTGTGAAT GAAAACACTA AGGAAGAAGA AGAAACCGAA 
GAATCAGAAA GTGATTCTGA ACGTGAGGAT CCACAGCCTG TTACAAATAT TAGAAATCCT 
CAAGGAATCA GTAGTTGGAG TGACATAAAT AGCAACTCAA ATGTACAGTG TGGAACTAAT 
AACAGAGATG GAAGACATCT TAATACAGAC TGTGAAATAA ATAACAGATC TGCTGCTAAC 
ATAAGGACTC TAAAAATGTC GTCACCTTTA GACTGTCATA ATAGGGAGGA TGACAAAGAT 
ATTGGCCTAC CCCAATGTGA AGATGAAGAA GTGGAAGAAG ATAGAGTTAG TGAAGATTCT 
GCATCTAGTC ACAGAAGCAG CCTGGGTGAT ACTGCTGGAG ATGCTGAGTT TGAGCAGAAG 
ATCAATAGGC TTATAGCTGC AAAACAGAAA CTTAGACAAT TACAAAACCT TGCTGCTATG 
GTGCAGGATG ATGATCCAGC ACCTCAGACA ACAATTGCAA ATGCATCTAA TATTGGTGAC 
TTGTTTTTGG GTGAGATGGA AGAGACAAAG CAGCAGCCAA ACAATGTCCG AGCTAGTACC 
AACAAATTAC AAAAAGATGT AGAACTGAAT GAAAAAGCAA GAGAGAAGTT TTATGAAGCT 
AAACTCCAGC AGCAGCAACA GGAGCTTAAG CAGTTACAAG AAGAAAGAAG AAAACTGATT 
GAAATTCAAG AAAAAATTCA GGTGTTACAG AAAGCTTGTC CTGATCTTCA ATTGTCAGCT 
GGCTTGGGTG ACTGCACAGC AAATAGACAG ACTTCACCAG CAACATCAAC TCCAGCCATG 
AATGACACAG CTGGCAAGCC TTCACTTGAA TTTGATGAAT CTGTACCAGT AGGCAATGAG 
TTATGGTCTG AGATGAGAAG ACATGAGATT TTAAGAGAAG AATTGCGACA AAGAAGAAAG 
CAACTGGAAG CTCTAATGGC TGAGCATCAG AGGAGGAAAG AGCTCGCAGA AACAATATCT 
ACTGTTGCTG CGTCTGTTAA AAGTGAAGGA TCAGAAGCTC GGCGTACTCC ACAGCAGAGT 
AGGACTGAAA AGTGGAAAAA TTACCGTCCT CTTTCAGCAG ATGGAAATTA TCGTCCATTG 
TCTAAGGCCA GACAACAGCA AAACATAAGT ATGCGACGTC AGGAAAACCT TCGGTGGATG 
TCTGAACTTT CATATGTGGA AGAAAAGGAA CGATGGCAAG AGCAGATCAA TCAGTTGAAG 
AAACAGCATG AATTTAGTGT CAGCATTTGT CAAACTTTAA TGCAGGATCA GCAGACCCTC 
TCTTGTCTTC TACAGACTTT GCTTACAGGC CCTTACAGTA TGATGCCCAA TAATGTTGCA 
TCTTCACAAG TACATCTTAT TATGCATCAG CTAAACCAGT GTTACACTCA ACTGACATGG 
CAGCAGAATA ACGTCCAGAG GTTGAAACAA ATGTTAAATG ATCTTGTGCG TCAGCAAGAA 
GAACAACGGT ATGAAGAGAA GTCATCAAGA AAGGATAGAG GCAGTGGTGT ACCCCCACCT 
CCATCTCCTG TTTTCTGTCC GTTCAGCTTT CCTCCACAAC CTTTGAACCT GTTTAATGTA 
CCAGGATTTA CTAATTTTTC TTCCTTTGCT CCAGATATTA ACTATAATCC AGTGTTTCCT 
TCTGGTTTTG GAGATTTTGC ACACAATATT TCTCCACACA GCAGTGAGCA GCAGCAGCAA 
CATCCTCTAG ATCATAATGC ATCTGGGAAA ACTGAGTATA TGGCGTTCCC CAAACCTTTT 
GAAAGCAGTT CCTCTAATGG AGCGGAAAAA CAAAGGAGGA GTCACAGACA ACCTGAAGAG 
GAAATGGAAA AAAGATCAGC TTGGCTTAAT GATAGCCAGG ACGTGAAAAA AGATGATCAG 
TCTCAGCTGA AAGCAAGTTT TGCTGTTTCA GTACAAAACA TTGCTTCCAA TCATAAAAAT 
CAGTCTGATA TGAGCAGAAG AAGAGAGTTT GATGAAGAGT CTTTGGAGAG TTTTAGTAGC 
ATGCCTGATC CAGTAGACCC CACTACTGTG ACAAAGACAT TTAAATCTAG AAAAGCATCA 
GCACAAGCAA GTTTGGCATC AAAAGATAAA ACACCTAAAT CGAAGAATAA GAGGAAGAGT 
TCTTCTCAGC TAAAAGGCAG AATTACAAAT ATTGATTATG AAAGTGCCAG TGCTTCTAGT 
GTGTGTGAAC CCTGCAAGAG CAATAAAAGC AGACACTCTG AAGAGGTTGG TCATGCAAAG 
GTGTTCAGCA AAAGGAATCG GGAACAACTG GAAAAAATCA TTAAATACAG TAGATCTACA 
GAAATGTCTT CAGCGCATGC TAGGAGAATT CTGCAGCAGT CTAACAGAAA TGCATGCATT 
GAAGCGCCAG AAACTGGTAG TGATCTTTCT ATGTTTGAAG CTTTACGAGA CACAATTTAT 
TCTGAAGTGG CAACTCTTAT TTCTCAAAAT GAGTCTCGTC CCCACTTTCT TATTGAACTT 
TTCCATGAGC TTCAGCTGCT AAATACAGAT TACCTGAGGC AAAGAGCTCT ATACGCTCTA 
CAGGATATAG TGACCAGACA TTTATCTGAG AACAATGAAA AAGGGAAGTG CACAAAATCA 
CTGAATTCTG CAGCATGGAT AGCATCAAAT TCTGAACTCA CTCCCAGTGA AAGCCTTGCT 
TCTACAGATG ATGAAACTTT TGGCAAAAAC TTTTCTACAG AAGCATGTCA AGATTGTGAA 
CAAAATGATG CAGACAATGG GAGTAGCATG TCTACATCTT CAAATTTTGA ACCCTTTGCC 
ACTGATGACC TTGGCAACAC AGTGATTCAC TTAGATCAAG CTTTGGCTAG GATGAGGGAG 
TATGAGCGTA TGAAAATTGA AGCTGAAAGT ACCCTTGACT CTGAGGGCTG CTCTAGTAAT 
TTTCAGGGTG CTTCTGCTGC TAAATTAGAA GGAGGTCAAA GTGCCAGTGA ATGTGTTCCT 
GTGCCACAGT CAAGTGAAGC TTCTGCTGTT CCATGTCCTC ATATAGACAC TCAGCAGCTT 
GACCGGCAGA TTAAAGCAAT TATGAAAGAG GTCATTCCTT TTCTGAAGGA ACACATGGAT 
GAAGTATGCT CTTCGCAGTT ACTGACATCA GTAAGACGTA TGGTCTTGAC CCTTACACAA 
CAAAATGATG AAAGTAAAGA GTTTGTGAAG TTCTTTCATA AGCAGCTTGG CAGTATACTT 
CAGGATTCAC TGGCAAAATT TGCTGGTAGA AAACTGAAAG ATTGTGGAGA GGATCTTCTA 
GTGGAGATCT CTGAAGTGTT ATTTAATGAA TTAGCCTTTT TTAAACTCAT GCAAGACTTG 
GACAACAACA GTATTTCTGT AAAGCAGAGA TGTAAAAGAA AAATAGAAGC TACTGAAGTG 
ATACAGTCGT ATGCTAAAGA GGCAAAAAAA GGTCTCCAGG TGGATGTTTG TTCATCTGCT 
GAAGATGTCG ATGAGGACAA AGACAAGGAT GAGACTGAAA CTGTTAAACA AGTACAGGAC 
TCAGAAATGT ATGATGGGAA TAGAGTTCCT GAAAGTATTA GGTCTGATGC ATCTGATCAG 
GAGGAAGATG ATGAAAGTGA AAGCGGTCCA GTGGCAATAA GTTTATCAAA AGCAGAAACC 
CAAGCTCTGA CTAACTATGG TAGTGGAGAA GATGAGAATG AAGATGAAGA AATAGAATTT 
GAGGAAGGAC CTGTTGATGT GCAGACGTCA TTACAAGCCA GCAGTGAAAC AACAACTGAA 
AATGAACAGA ATTCAAACCA AGAACAAAGT AAGACAAAAA GCAGTGAGAT TCTGTCATCA 
GAACAAGAAT CTGTTTATGC TAAAGGTGAA CAACATGTGG CTACAATTTT GCCTTATTAC 
CTCAATGTAA TGGAGAATGC ACCGCCTTTA ACGGTCAATA CCCCAGAATC TTTTATAACA 
GACAGTATGA AAACAGAAGA ATCAAGTTCA TCTTTACCAG TAAATGAAAC TCAAACACTG 
GATACAACGT GTGTAGGAAA CAAATCTGCT GCAAGTTCTG AAAGCTCCAT GGCTGGCAGC 
CCTGATACAG AGTCACCTGT GTTAGTGAAT GAATATGAAG CTGGTTCTGG AAATGTAAGT 
CAAAGATCTG ATGAAGATGA CTTTGTGAAA GTTGAAGACT TGCCCCTTAA ACTTGCAGTG 
TATTCAGAGG CAGATTTAAT GAAGAAAATG GCAATGGAGG CCCAAACGAA CAGTTTGTCT 
GATGAATTAC TGGGTGGAGG TGGAGCTCAA GATCAAGAAT TAGTAGGAGA TGCCCAAACT 
TTGAAAGAAC CTGAAACTTT TGGAGTTCAA AGTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_021285865.1 ) cDNA ORF clone, Columba livia(rock pigeon) -> XP_021141540.1 Columba livia pericentriolar material 1 (PCM1), transcript variant X12, mRNA.

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CloneID	OCo293168
Clone ID Related Accession (Same CDS sequence)	XM_021285865.1
Accession Version	XM_021285865.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6114bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-23
Organism	Columba livia(rock pigeon)
Product	pericentriolar material 1 protein isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004973253.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Columba livia Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACAG GAGGTGGTCC CTTTGAAGAA GGCATGAATG ATCAAGACTT GCCTAGCTGG 
AGCAATGACA GCTTTGATGA CCGGCTGAAC AACACGGACT GGGGAAGTCA ACAGAAGAAA 
GCAAACAGAT CTTCAGAAAA AAACAAGAAA AAGCTTGGTG GGGAAGCTGA AACAAGGCTT 
ACTAATGATA TATCTCCAGA ATCCTCACCT GGAATAGGAC GACGAAAGAC CAGAACTCCT 
CATAGTTTTC CTCATGCTCG ATACATGACC CAGATGTCTG TTCCGGAGCA GGCTGAGCTA 
GAAAGGCTTA AACAAAGAAT AAACTTCAGT GATCTGGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGCA GGGCAACAGC TGCTAACAAC AAACGTCAAC TTAATGAAAA CAAAAAACCA 
TTCAACTTCC TGTCACTGCA GATAAACACT AACAAGAGCA AAGATCCTGC CTCATGTTCC 
CAAAAAAAGG AAAGTGGGGT GTCAGTCCAA CGTAAAGAGT TGTTTGGTGC TGCTCTGAGC 
AAGGATTTCT TGCAAAATTG TCAAGTCTCT GCTCAAGAAG ATGGAGGGGG AGAACCAGCT 
ATGGATAGTA GCCAGATTGT GAGCAGACTA GTTCAAATTC GCGACTATAT TGCTAAGGCC 
AGCTCCATGC GGGATGATCT TGTAGAGAAA AATGAAAGAT CGGCCAATGT TGAGCGTTTA 
TCACACCTTA TAGATCACCT TAAAGAGCAG GAGAAATCCT ATCTGAAATT TTTGCAAAAG 
ATGCTTGCTA GAGATCCTCA ACAGGAGGCT AAAGAGGAGT TGGAGAACTT GAAGAAGCAG 
CATGATTTAT TGAAAAGGAT GCTAAAACAG CAAGAGCAAT TAAAGGCTCT TCAAGGAAGA 
CAGGCAGCTC TTCTTGCTTT GCAGCATAAA GCAGAGCAAG CAATTGCTGT CATGGATGAT 
TCTGTTGTAA CAGAGACTAC AGGTAGTGTT TCAGGAGTAA GCCTTACATC AGAACTGAAT 
GAAGAATTGA ATGACTTAAT TCAGCGCTTT CACAACCAAC TTCATGATTC TCAGACACAG 
TCTGTACCAG ACAATAGAAG GCAAGCAGAA AGTCTTTCAC TTACTAGAGA GGTTTCACAA 
AGCAGAAACT CTTCAATGTC TGAACGCCTG TCAGATGAGA AGGCGCAGCT TTTTAACAAG 
ATGCGAATGT TGCAGGGTAA AAAGCAAAAA ATGGACAAAC TGTTAGGAGA ACTTCATACA 
CTTCGTGACC AACATCTAAA TAACTCCTCC TTTTTTCCTG CTTCAGGTTC TCCTCAAAGA 
AGTATTGATC AAAGAAGTAC AACTTCAGCT GCTTCTGGTC CTGCAGGCAT AGTAACTGTT 
GTCAATGGCG AATCAAATAG CCTGGCATCT GCTCCCTATC CTCCTGATTC CCTGGTTTCT 
CAGAATGAGA GTGAAGAAGA TGACAATCTA AATCCAACAG AAAAGCTTCA GAAACTAAAT 
GAAGTTCGTA AGAGGCTGAA TGAGTTACGT GAGCTAGTTC ACTACTATGA GCAAACATCT 
GATATGATGA CAGATTCTGT GAATGAAAAC ACTAAGGAAG AAGAAGAAAC CGAAGAATCA 
GAAAGTGATT CTGAACGTGA GGATCCACAG CCTGTTACAA ATATTAGAAA TCCTCAAGGA 
ATCAGTAGTT GGAGTGACAT AAATAGCAAC TCAAATGTAC AGTGTGGAAC TAATAACAGA 
GATGGAAGAC ATCTTAATAC AGACTGTGAA ATAAATAACA GATCTGCTGC TAACATAAGG 
ACTCTAAAAA TGTCGTCACC TTTAGACTGT CATAATAGGG AGGATGACAA AGATATTGGC 
CTACCCCAAT GTGAAGATGA AGAAGTGGAA GAAGATAGAG TTAGTGAAGA TTCTGCATCT 
AGTCACAGAA GCAGCCTGGG TGATACTGCT GGAGATGCTG AGTTTGAGCA GAAGATCAAT 
AGGCTTATAG CTGCAAAACA GAAACTTAGA CAATTACAAA ACCTTGCTGC TATGGTGCAG 
GATGATGATC CAGCACCTCA GACAACAATT GCAAATGCAT CTAATATTGG TGACTTGTTT 
TTGGGTGAGA TGGAAGAGAC AAAGCAGCAG CCAAACAATG TCCGAGCTAG TACCAACAAA 
TTACAAAAAG ATGTAGAACT GAATGAAAAA GCAAGAGAGA AGTTTTATGA AGCTAAACTC 
CAGCAGCAGC AACAGGAGCT TAAGCAGTTA CAAGAAGAAA GAAGAAAACT GATTGAAATT 
CAAGAAAAAA TTCAGGTGTT ACAGAAAGCT TGTCCTGATC TTCAATTGTC AGCTGGCTTG 
GGTGACTGCA CAGCAAATAG ACAGACTTCA CCAGCAACAT CAACTCCAGC CATGAATGAC 
ACAGCTGGCA AGCCTTCACT TGAATTTGAT GAATCTGTAC CAGTAGGCAA TGAGTTATGG 
TCTGAGATGA GAAGACATGA GATTTTAAGA GAAGAATTGC GACAAAGAAG AAAGCAACTG 
GAAGCTCTAA TGGCTGAGCA TCAGAGGAGG AAAGAGCTCG CAGAAACAAT ATCTACTGTT 
GCTGCGTCTG TTAAAAGTGA AGGATCAGAA GCTCGGCGTA CTCCACAGCA GAGTAGGACT 
GAAAATAGGA CAATGGCTAC CTGGGGAGGT TCTACGCAGT GTGCACTAGA TGAAGAGGAT 
GGAGATGAAG ATGGTTATCT CTCTGATGGA GCTGGTCAGG CAGAAGAAGA TGATGATGAT 
GCGTCAAGTG CGAATGACAG CTTCTCTGTT TATCCCAATA ACAACGTACC AGAAAATGCA 
TATTTTGTTA AAGAAAACAA GGATAGGTGG AAAAATTACC GTCCTCTTTC AGCAGATGGA 
AATTATCGTC CATTGTCTAA GGCCAGACAA CAGCAAAACA TAAGTATGCG ACGTCAGGAA 
AACCTTCGGT GGATGTCTGA ACTTTCATAT GTGGAAGAAA AGGAACGATG GCAAGAGCAG 
ATCAATCAGT TGAAGAAACA GCATGAATTT AGTGTCAGCA TTTGTCAAAC TTTAATGCAG 
GATCAGCAGA CCCTCTCTTG TCTTCTACAG ACTTTGCTTA CAGGCCCTTA CAGTATGATG 
CCCAATAATG TTGCATCTTC ACAAGTACAT CTTATTATGC ATCAGCTAAA CCAGTGTTAC 
ACTCAACTGA CATGGCAGCA GAATAACGTC CAGAGGTTGA AACAAATGTT AAATGATCTT 
GTGCGTCAGC AAGAAGAACA ACGGTATGAA GAGAAGTCAT CAAGAAAGGA TAGAGGCAGT 
GGTGTACCCC CACCTCCATC TCCTGTTTTC TGTCCGTTCA GCTTTCCTCC ACAACCTTTG 
AACCTGTTTA ATGTACCAGG ATTTACTAAT TTTTCTTCCT TTGCTCCAGA TATTAACTAT 
AATCCAGTGT TTCCTTCTGG TTTTGGAGAT TTTGCACACA ATATTTCTCC ACACAGCAGT 
GAGCAGCAGC AGCAACATCC TCTAGATCAT AATGCATCTG GGAAAACTGA GTATATGGCG 
TTCCCCAAAC CTTTTGAAAG CAGTTCCTCT AATGGAGCGG AAAAACAAAG GAGGAGTCAC 
AGACAACCTG AAGAGGAAAT GGAAAAAAGA TCAGCTTGGC TTAATGATAG CCAGGACGTG 
AAAAAAGATG ATCAGTCTCA GCTGAAAGCA AGTTTTGCTG TTTCAGTACA AAACATTGCT 
TCCAATCATA AAAATCAGTC TGATATGAGC AGAAGAAGAG AGTTTGATGA AGAGTCTTTG 
GAGAGTTTTA GTAGCATGCC TGATCCAGTA GACCCCACTA CTGTGACAAA GACATTTAAA 
TCTAGAAAAG CATCAGCACA AGCAAGTTTG GCATCAAAAG ATAAAACACC TAAATCGAAG 
AATAAGAGGA AGAGTTCTTC TCAGCTAAAA GGCAGAATTA CAAATATTGA TTATGAAAGT 
GCCAGTGCTT CTAGTGTGTG TGAACCCTGC AAGAGCAATA AAAGCAGACA CTCTGAAGAG 
GTTGGTCATG CAAAGGTGTT CAGCAAAAGG AATCGGGAAC AACTGGAAAA AATCATTAAA 
TACAGTAGAT CTACAGAAAT GTCTTCAGCG CATGCTAGGA GAATTCTGCA GCAGTCTAAC 
AGAAATGCAT GCATTGAAGC GCCAGAAACT GGTAGTGATC TTTCTATGTT TGAAGCTTTA 
CGAGACACAA TTTATTCTGA AGTGGCAACT CTTATTTCTC AAAATGAGTC TCGTCCCCAC 
TTTCTTATTG AACTTTTCCA TGAGCTTCAG CTGCTAAATA CAGATTACCT GAGGCAAAGA 
GCTCTATACG CTCTACAGGA TATAGTGACC AGACATTTAT CTGAGAACAA TGAAAAAGGG 
AAGTGCACAA AATCACTGAA TTCTGCAGCA TGGATAGCAT CAAATTCTGA ACTCACTCCC 
AGTGAAAGCC TTGCTTCTAC AGATGATGAA ACTTTTGGCA AAAACTTTTC TACAGAAGCA 
TGTCAAGATT GTGAACAAAA TGATGCAGAC AATGGGAGTA GCATGTCTAC ATCTTCAAAT 
TTTGAACCCT TTGCCACTGA TGACCTTGGC AACACAGTGA TTCACTTAGA TCAAGCTTTG 
GCTAGGATGA GGGAGTATGA GCGTATGAAA ATTGAAGCTG AAAGTACCCT TGACTCTGAG 
GGCTGCTCTA GTAATTTTCA GGGTGCTTCT GCTGCTAAAT TAGAAGGAGG TCAAAGTGCC 
AGTGAATGTG TTCCTGTGCC ACAGTCAAGT GAAGCTTCTG CTGTTCCATG TCCTCATATA 
GACACTCAGC AGCTTGACCG GCAGATTAAA GCAATTATGA AAGAGGTCAT TCCTTTTCTG 
AAGGAACACA TGGATGAAGT ATGCTCTTCG CAGTTACTGA CATCAGTAAG ACGTATGGTC 
TTGACCCTTA CACAACAAAA TGATGAAAGT AAAGAGTTTG TGAAGTTCTT TCATAAGCAG 
CTTGGCAGTA TACTTCAGGA TTCACTGGCA AAATTTGCTG GTAGAAAACT GAAAGATTGT 
GGAGAGGATC TTCTAGTGGA GATCTCTGAA GTGTTATTTA ATGAATTAGC CTTTTTTAAA 
CTCATGCAAG ACTTGGACAA CAACAGTATT TCTGTAAAGC AGAGATGTAA AAGAAAAATA 
GAAGCTACTG AAGTGATACA GTCGTATGCT AAAGAGGCAA AAAAAGGTCT CCAGGTGGAT 
GTTTGTTCAT CTGCTGAAGA TGTCGATGAG GACAAAGACA AGGATGAGAC TGAAACTGTT 
AAACAAGTAC AGGACTCAGA AATGTATGAT GGGAATAGAG TTCCTGAAAG TATTAGGTCT 
GATGCATCTG ATCAGGAGGA AGATGATGAA AGTGAAAGCG GTCCAGTGGC AATAAGTTTA 
TCAAAAGCAG AAACCCAAGC TCTGACTAAC TATGGTAGTG GAGAAGATGA GAATGAAGAT 
GAAGAAATAG AATTTGAGGA AGGACCTGTT GATGTGCAGA CGTCATTACA AGCCAGCAGT 
GAAACAACAA CTGAAAATGA ACAGAATTCA AACCAAGAAC AAAGTAAGAC AAAAAGCAGT 
GAGATTCTGT CATCAGAACA AGAATCTGTT TATGCTAAAG GTGAACAACA TGTGGCTACA 
ATTTTGCCTT ATTACCTCAA TGTAATGGAG AATGCACCGC CTTTAACGGT CAATACCCCA 
GAATCTTTTA TAACAGACAG TATGAAAACA GAAGAATCAA GTTCATCTTT ACCAGTAAAT 
GAAACTCAAA CACTGGATAC AACGTGTGTA GGAAACAAAT CTGCTGCAAG TTCTGAAAGC 
TCCATGGCTG GCAGCCCTGA TACAGAGTCA CCTGTGTTAG TGAATGAATA TGAAGCTGGT 
TCTGGAAATG TAAGTCAAAG ATCTGATGAA GATGACTTTG TGAAAGTTGA AGACTTGCCC 
CTTAAACTTG CAGTGTATTC AGAGGCAGAT TTAATGAAGA AAATGGCAAT GGAGGCCCAA 
ACGAACAGTT TGTCTGATGA ATTACTGGGT GGAGGTGGAG CTCAAGATCA AGAATTAGTA 
GGAGATGCCC AAACTTTGAA AGAACCTGAA ACTTTTGGAG TTCAAAGTGC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021141540.1
CDS	140..6253
Translation	MATGGGPFEEGMNDQDLPSWSNDSFDDRLNNTDWGSQQKKANRSSEKNKKKLGGEAETRLTNDISPESSPGIGRRKTRTPHSFPHARYMTQMSVPEQAELERLKQRINFSDLDQRSIGSDSQGRATAANNKRQLNENKKPFNFLSLQINTNKSKDPASCSQKKESGVSVQRKELFGAALSKDFLQNCQVSAQEDGGGEPAMDSSQIVSRLVQIRDYIAKASSMRDDLVEKNERSANVERLSHLIDHLKEQEKSYLKFLQKMLARDPQQEAKEELENLKKQHDLLKRMLKQQEQLKALQGRQAALLALQHKAEQAIAVMDDSVVTETTGSVSGVSLTSELNEELNDLIQRFHNQLHDSQTQSVPDNRRQAESLSLTREVSQSRNSSMSERLSDEKAQLFNKMRMLQGKKQKMDKLLGELHTLRDQHLNNSSFFPASGSPQRSIDQRSTTSAASGPAGIVTVVNGESNSLASAPYPPDSLVSQNESEEDDNLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDSVNENTKEEEETEESESDSEREDPQPVTNIRNPQGISSWSDINSNSNVQCGTNNRDGRHLNTDCEINNRSAANIRTLKMSSPLDCHNREDDKDIGLPQCEDEEVEEDRVSEDSASSHRSSLGDTAGDAEFEQKINRLIAAKQKLRQLQNLAAMVQDDDPAPQTTIANASNIGDLFLGEMEETKQQPNNVRASTNKLQKDVELNEKAREKFYEAKLQQQQQELKQLQEERRKLIEIQEKIQVLQKACPDLQLSAGLGDCTANRQTSPATSTPAMNDTAGKPSLEFDESVPVGNELWSEMRRHEILREELRQRRKQLEALMAEHQRRKELAETISTVAASVKSEGSEARRTPQQSRTENRTMATWGGSTQCALDEEDGDEDGYLSDGAGQAEEDDDDASSANDSFSVYPNNNVPENAYFVKENKDRWKNYRPLSADGNYRPLSKARQQQNISMRRQENLRWMSELSYVEEKERWQEQINQLKKQHEFSVSICQTLMQDQQTLSCLLQTLLTGPYSMMPNNVASSQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNDLVRQQEEQRYEEKSSRKDRGSGVPPPPSPVFCPFSFPPQPLNLFNVPGFTNFSSFAPDINYNPVFPSGFGDFAHNISPHSSEQQQQHPLDHNASGKTEYMAFPKPFESSSSNGAEKQRRSHRQPEEEMEKRSAWLNDSQDVKKDDQSQLKASFAVSVQNIASNHKNQSDMSRRREFDEESLESFSSMPDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKNKRKSSSQLKGRITNIDYESASASSVCEPCKSNKSRHSEEVGHAKVFSKRNREQLEKIIKYSRSTEMSSAHARRILQQSNRNACIEAPETGSDLSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHLSENNEKGKCTKSLNSAAWIASNSELTPSESLASTDDETFGKNFSTEACQDCEQNDADNGSSMSTSSNFEPFATDDLGNTVIHLDQALARMREYERMKIEAESTLDSEGCSSNFQGASAAKLEGGQSASECVPVPQSSEASAVPCPHIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSISVKQRCKRKIEATEVIQSYAKEAKKGLQVDVCSSAEDVDEDKDKDETETVKQVQDSEMYDGNRVPESIRSDASDQEEDDESESGPVAISLSKAETQALTNYGSGEDENEDEEIEFEEGPVDVQTSLQASSETTTENEQNSNQEQSKTKSSEILSSEQESVYAKGEQHVATILPYYLNVMENAPPLTVNTPESFITDSMKTEESSSSLPVNETQTLDTTCVGNKSAASSESSMAGSPDTESPVLVNEYEAGSGNVSQRSDEDDFVKVEDLPLKLAVYSEADLMKKMAMEAQTNSLSDELLGGGGAQDQELVGDAQTLKEPETFGVQSA

Target ORF information:

RefSeq Version	XM_021285865.1
Organism	Columba livia(rock pigeon)
Definition	Columba livia pericentriolar material 1 (PCM1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021285865.1

ORF Insert Sequence:

ATGGCAACAG GAGGTGGTCC CTTTGAAGAA GGCATGAATG ATCAAGACTT GCCTAGCTGG 
AGCAATGACA GCTTTGATGA CCGGCTGAAC AACACGGACT GGGGAAGTCA ACAGAAGAAA 
GCAAACAGAT CTTCAGAAAA AAACAAGAAA AAGCTTGGTG GGGAAGCTGA AACAAGGCTT 
ACTAATGATA TATCTCCAGA ATCCTCACCT GGAATAGGAC GACGAAAGAC CAGAACTCCT 
CATAGTTTTC CTCATGCTCG ATACATGACC CAGATGTCTG TTCCGGAGCA GGCTGAGCTA 
GAAAGGCTTA AACAAAGAAT AAACTTCAGT GATCTGGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGCA GGGCAACAGC TGCTAACAAC AAACGTCAAC TTAATGAAAA CAAAAAACCA 
TTCAACTTCC TGTCACTGCA GATAAACACT AACAAGAGCA AAGATCCTGC CTCATGTTCC 
CAAAAAAAGG AAAGTGGGGT GTCAGTCCAA CGTAAAGAGT TGTTTGGTGC TGCTCTGAGC 
AAGGATTTCT TGCAAAATTG TCAAGTCTCT GCTCAAGAAG ATGGAGGGGG AGAACCAGCT 
ATGGATAGTA GCCAGATTGT GAGCAGACTA GTTCAAATTC GCGACTATAT TGCTAAGGCC 
AGCTCCATGC GGGATGATCT TGTAGAGAAA AATGAAAGAT CGGCCAATGT TGAGCGTTTA 
TCACACCTTA TAGATCACCT TAAAGAGCAG GAGAAATCCT ATCTGAAATT TTTGCAAAAG 
ATGCTTGCTA GAGATCCTCA ACAGGAGGCT AAAGAGGAGT TGGAGAACTT GAAGAAGCAG 
CATGATTTAT TGAAAAGGAT GCTAAAACAG CAAGAGCAAT TAAAGGCTCT TCAAGGAAGA 
CAGGCAGCTC TTCTTGCTTT GCAGCATAAA GCAGAGCAAG CAATTGCTGT CATGGATGAT 
TCTGTTGTAA CAGAGACTAC AGGTAGTGTT TCAGGAGTAA GCCTTACATC AGAACTGAAT 
GAAGAATTGA ATGACTTAAT TCAGCGCTTT CACAACCAAC TTCATGATTC TCAGACACAG 
TCTGTACCAG ACAATAGAAG GCAAGCAGAA AGTCTTTCAC TTACTAGAGA GGTTTCACAA 
AGCAGAAACT CTTCAATGTC TGAACGCCTG TCAGATGAGA AGGCGCAGCT TTTTAACAAG 
ATGCGAATGT TGCAGGGTAA AAAGCAAAAA ATGGACAAAC TGTTAGGAGA ACTTCATACA 
CTTCGTGACC AACATCTAAA TAACTCCTCC TTTTTTCCTG CTTCAGGTTC TCCTCAAAGA 
AGTATTGATC AAAGAAGTAC AACTTCAGCT GCTTCTGGTC CTGCAGGCAT AGTAACTGTT 
GTCAATGGCG AATCAAATAG CCTGGCATCT GCTCCCTATC CTCCTGATTC CCTGGTTTCT 
CAGAATGAGA GTGAAGAAGA TGACAATCTA AATCCAACAG AAAAGCTTCA GAAACTAAAT 
GAAGTTCGTA AGAGGCTGAA TGAGTTACGT GAGCTAGTTC ACTACTATGA GCAAACATCT 
GATATGATGA CAGATTCTGT GAATGAAAAC ACTAAGGAAG AAGAAGAAAC CGAAGAATCA 
GAAAGTGATT CTGAACGTGA GGATCCACAG CCTGTTACAA ATATTAGAAA TCCTCAAGGA 
ATCAGTAGTT GGAGTGACAT AAATAGCAAC TCAAATGTAC AGTGTGGAAC TAATAACAGA 
GATGGAAGAC ATCTTAATAC AGACTGTGAA ATAAATAACA GATCTGCTGC TAACATAAGG 
ACTCTAAAAA TGTCGTCACC TTTAGACTGT CATAATAGGG AGGATGACAA AGATATTGGC 
CTACCCCAAT GTGAAGATGA AGAAGTGGAA GAAGATAGAG TTAGTGAAGA TTCTGCATCT 
AGTCACAGAA GCAGCCTGGG TGATACTGCT GGAGATGCTG AGTTTGAGCA GAAGATCAAT 
AGGCTTATAG CTGCAAAACA GAAACTTAGA CAATTACAAA ACCTTGCTGC TATGGTGCAG 
GATGATGATC CAGCACCTCA GACAACAATT GCAAATGCAT CTAATATTGG TGACTTGTTT 
TTGGGTGAGA TGGAAGAGAC AAAGCAGCAG CCAAACAATG TCCGAGCTAG TACCAACAAA 
TTACAAAAAG ATGTAGAACT GAATGAAAAA GCAAGAGAGA AGTTTTATGA AGCTAAACTC 
CAGCAGCAGC AACAGGAGCT TAAGCAGTTA CAAGAAGAAA GAAGAAAACT GATTGAAATT 
CAAGAAAAAA TTCAGGTGTT ACAGAAAGCT TGTCCTGATC TTCAATTGTC AGCTGGCTTG 
GGTGACTGCA CAGCAAATAG ACAGACTTCA CCAGCAACAT CAACTCCAGC CATGAATGAC 
ACAGCTGGCA AGCCTTCACT TGAATTTGAT GAATCTGTAC CAGTAGGCAA TGAGTTATGG 
TCTGAGATGA GAAGACATGA GATTTTAAGA GAAGAATTGC GACAAAGAAG AAAGCAACTG 
GAAGCTCTAA TGGCTGAGCA TCAGAGGAGG AAAGAGCTCG CAGAAACAAT ATCTACTGTT 
GCTGCGTCTG TTAAAAGTGA AGGATCAGAA GCTCGGCGTA CTCCACAGCA GAGTAGGACT 
GAAAATAGGA CAATGGCTAC CTGGGGAGGT TCTACGCAGT GTGCACTAGA TGAAGAGGAT 
GGAGATGAAG ATGGTTATCT CTCTGATGGA GCTGGTCAGG CAGAAGAAGA TGATGATGAT 
GCGTCAAGTG CGAATGACAG CTTCTCTGTT TATCCCAATA ACAACGTACC AGAAAATGCA 
TATTTTGTTA AAGAAAACAA GGATAGGTGG AAAAATTACC GTCCTCTTTC AGCAGATGGA 
AATTATCGTC CATTGTCTAA GGCCAGACAA CAGCAAAACA TAAGTATGCG ACGTCAGGAA 
AACCTTCGGT GGATGTCTGA ACTTTCATAT GTGGAAGAAA AGGAACGATG GCAAGAGCAG 
ATCAATCAGT TGAAGAAACA GCATGAATTT AGTGTCAGCA TTTGTCAAAC TTTAATGCAG 
GATCAGCAGA CCCTCTCTTG TCTTCTACAG ACTTTGCTTA CAGGCCCTTA CAGTATGATG 
CCCAATAATG TTGCATCTTC ACAAGTACAT CTTATTATGC ATCAGCTAAA CCAGTGTTAC 
ACTCAACTGA CATGGCAGCA GAATAACGTC CAGAGGTTGA AACAAATGTT AAATGATCTT 
GTGCGTCAGC AAGAAGAACA ACGGTATGAA GAGAAGTCAT CAAGAAAGGA TAGAGGCAGT 
GGTGTACCCC CACCTCCATC TCCTGTTTTC TGTCCGTTCA GCTTTCCTCC ACAACCTTTG 
AACCTGTTTA ATGTACCAGG ATTTACTAAT TTTTCTTCCT TTGCTCCAGA TATTAACTAT 
AATCCAGTGT TTCCTTCTGG TTTTGGAGAT TTTGCACACA ATATTTCTCC ACACAGCAGT 
GAGCAGCAGC AGCAACATCC TCTAGATCAT AATGCATCTG GGAAAACTGA GTATATGGCG 
TTCCCCAAAC CTTTTGAAAG CAGTTCCTCT AATGGAGCGG AAAAACAAAG GAGGAGTCAC 
AGACAACCTG AAGAGGAAAT GGAAAAAAGA TCAGCTTGGC TTAATGATAG CCAGGACGTG 
AAAAAAGATG ATCAGTCTCA GCTGAAAGCA AGTTTTGCTG TTTCAGTACA AAACATTGCT 
TCCAATCATA AAAATCAGTC TGATATGAGC AGAAGAAGAG AGTTTGATGA AGAGTCTTTG 
GAGAGTTTTA GTAGCATGCC TGATCCAGTA GACCCCACTA CTGTGACAAA GACATTTAAA 
TCTAGAAAAG CATCAGCACA AGCAAGTTTG GCATCAAAAG ATAAAACACC TAAATCGAAG 
AATAAGAGGA AGAGTTCTTC TCAGCTAAAA GGCAGAATTA CAAATATTGA TTATGAAAGT 
GCCAGTGCTT CTAGTGTGTG TGAACCCTGC AAGAGCAATA AAAGCAGACA CTCTGAAGAG 
GTTGGTCATG CAAAGGTGTT CAGCAAAAGG AATCGGGAAC AACTGGAAAA AATCATTAAA 
TACAGTAGAT CTACAGAAAT GTCTTCAGCG CATGCTAGGA GAATTCTGCA GCAGTCTAAC 
AGAAATGCAT GCATTGAAGC GCCAGAAACT GGTAGTGATC TTTCTATGTT TGAAGCTTTA 
CGAGACACAA TTTATTCTGA AGTGGCAACT CTTATTTCTC AAAATGAGTC TCGTCCCCAC 
TTTCTTATTG AACTTTTCCA TGAGCTTCAG CTGCTAAATA CAGATTACCT GAGGCAAAGA 
GCTCTATACG CTCTACAGGA TATAGTGACC AGACATTTAT CTGAGAACAA TGAAAAAGGG 
AAGTGCACAA AATCACTGAA TTCTGCAGCA TGGATAGCAT CAAATTCTGA ACTCACTCCC 
AGTGAAAGCC TTGCTTCTAC AGATGATGAA ACTTTTGGCA AAAACTTTTC TACAGAAGCA 
TGTCAAGATT GTGAACAAAA TGATGCAGAC AATGGGAGTA GCATGTCTAC ATCTTCAAAT 
TTTGAACCCT TTGCCACTGA TGACCTTGGC AACACAGTGA TTCACTTAGA TCAAGCTTTG 
GCTAGGATGA GGGAGTATGA GCGTATGAAA ATTGAAGCTG AAAGTACCCT TGACTCTGAG 
GGCTGCTCTA GTAATTTTCA GGGTGCTTCT GCTGCTAAAT TAGAAGGAGG TCAAAGTGCC 
AGTGAATGTG TTCCTGTGCC ACAGTCAAGT GAAGCTTCTG CTGTTCCATG TCCTCATATA 
GACACTCAGC AGCTTGACCG GCAGATTAAA GCAATTATGA AAGAGGTCAT TCCTTTTCTG 
AAGGAACACA TGGATGAAGT ATGCTCTTCG CAGTTACTGA CATCAGTAAG ACGTATGGTC 
TTGACCCTTA CACAACAAAA TGATGAAAGT AAAGAGTTTG TGAAGTTCTT TCATAAGCAG 
CTTGGCAGTA TACTTCAGGA TTCACTGGCA AAATTTGCTG GTAGAAAACT GAAAGATTGT 
GGAGAGGATC TTCTAGTGGA GATCTCTGAA GTGTTATTTA ATGAATTAGC CTTTTTTAAA 
CTCATGCAAG ACTTGGACAA CAACAGTATT TCTGTAAAGC AGAGATGTAA AAGAAAAATA 
GAAGCTACTG AAGTGATACA GTCGTATGCT AAAGAGGCAA AAAAAGGTCT CCAGGTGGAT 
GTTTGTTCAT CTGCTGAAGA TGTCGATGAG GACAAAGACA AGGATGAGAC TGAAACTGTT 
AAACAAGTAC AGGACTCAGA AATGTATGAT GGGAATAGAG TTCCTGAAAG TATTAGGTCT 
GATGCATCTG ATCAGGAGGA AGATGATGAA AGTGAAAGCG GTCCAGTGGC AATAAGTTTA 
TCAAAAGCAG AAACCCAAGC TCTGACTAAC TATGGTAGTG GAGAAGATGA GAATGAAGAT 
GAAGAAATAG AATTTGAGGA AGGACCTGTT GATGTGCAGA CGTCATTACA AGCCAGCAGT 
GAAACAACAA CTGAAAATGA ACAGAATTCA AACCAAGAAC AAAGTAAGAC AAAAAGCAGT 
GAGATTCTGT CATCAGAACA AGAATCTGTT TATGCTAAAG GTGAACAACA TGTGGCTACA 
ATTTTGCCTT ATTACCTCAA TGTAATGGAG AATGCACCGC CTTTAACGGT CAATACCCCA 
GAATCTTTTA TAACAGACAG TATGAAAACA GAAGAATCAA GTTCATCTTT ACCAGTAAAT 
GAAACTCAAA CACTGGATAC AACGTGTGTA GGAAACAAAT CTGCTGCAAG TTCTGAAAGC 
TCCATGGCTG GCAGCCCTGA TACAGAGTCA CCTGTGTTAG TGAATGAATA TGAAGCTGGT 
TCTGGAAATG TAAGTCAAAG ATCTGATGAA GATGACTTTG TGAAAGTTGA AGACTTGCCC 
CTTAAACTTG CAGTGTATTC AGAGGCAGAT TTAATGAAGA AAATGGCAAT GGAGGCCCAA 
ACGAACAGTT TGTCTGATGA ATTACTGGGT GGAGGTGGAG CTCAAGATCA AGAATTAGTA 
GGAGATGCCC AAACTTTGAA AGAACCTGAA ACTTTTGGAG TTCAAAGTGC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_021285866.1 ) cDNA ORF clone, Columba livia(rock pigeon) -> XP_021141541.1 Columba livia pericentriolar material 1 (PCM1), transcript variant X13, mRNA.

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CloneID	OCo293169
Clone ID Related Accession (Same CDS sequence)	XM_021285866.1
Accession Version	XM_021285866.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6111bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-23
Organism	Columba livia(rock pigeon)
Product	pericentriolar material 1 protein isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004973253.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Columba livia Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACAG GAGGTGGTCC CTTTGAAGAA GGCATGAATG ATCAAGACTT GCCTAGCTGG 
AGCAATGACA GCTTTGATGA CCGGCTGAAC AACACGGACT GGGGAAGTCA ACAGAAGAAA 
GCAAACAGAT CTTCAGAAAA AAACAAGAAA AAGCTTGGTG GGGAAGCTGA AACAAGGCTT 
ACTAATGATA TATCTCCAGA ATCCTCACCT GGAATAGGAC GACGAAAGAC CAGAACTCCT 
CATAGTTTTC CTCATGCTCG ATACATGACC CAGATGTCTG TTCCGGAGCA GGCTGAGCTA 
GAAAGGCTTA AACAAAGAAT AAACTTCAGT GATCTGGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGCA GGGCAACAGC TGCTAACAAC AAACGTCAAC TTAATGAAAA CAAAAAACCA 
TTCAACTTCC TGTCACTGCA GATAAACACT AACAAGAGCA AAGATCCTGC CTCATGTTCC 
CAAAAAAAGG AAAGTGGGGT GTCAGTCCAA CGTAAAGAGT TGTTTGGTGC TGCTCTGAGC 
AAGGATTTCT TGCAAAATTG TCAAGTCTCT GCTCAAGAAG ATGGAGGGGG AGAACCAGCT 
ATGGATAGTA GCCAGATTGT GAGCAGACTA GTTCAAATTC GCGACTATAT TGCTAAGGCC 
AGCTCCATGC GGGATGATCT TGTAGAGAAA AATGAAAGAT CGGCCAATGT TGAGCGTTTA 
TCACACCTTA TAGATCACCT TAAAGAGCAG GAGAAATCCT ATCTGAAATT TTTGCAAAAG 
ATGCTTGCTA GAGATCCTCA ACAGGAGGCT AAAGAGGAGT TGGAGAACTT GAAGAAGCAG 
CATGATTTAT TGAAAAGGAT GCTAAAACAG CAAGAGCAAT TAAAGGCTCT TCAAGGAAGA 
CAGGCAGCTC TTCTTGCTTT GCAGCATAAA GCAGAGCAAG CAATTGCTGT CATGGATGAT 
TCTGTTGTAA CAGAGACTAC AGGTAGTGTT TCAGGAGTAA GCCTTACATC AGAACTGAAT 
GAAGAATTGA ATGACTTAAT TCAGCGCTTT CACAACCAAC TTCATGATTC TCAGACACAG 
TCTGTACCAG ACAATAGAAG GCAAGCAGAA AGTCTTTCAC TTACTAGAGA GGTTTCACAA 
AGCAGAAACT CTTCAATGTC TGAACGCCTG TCAGATGAGA AGGCGCAGCT TTTTAACAAG 
ATGCGAATGT TGCAGGGTAA AAAGCAAAAA ATGGACAAAC TGTTAGGAGA ACTTCATACA 
CTTCGTGACC AACATCTAAA TAACTCCTCC TTTTTTCCTG CTTCAGGTTC TCCTCAAAGA 
AGTATTGATC AAAGAAGTAC AACTTCAGCT GCTTCTGGTC CTGCAGGCAT AGTAACTGTT 
GTCAATGGCG AATCAAATAG CCTGGCATCT GCTCCCTATC CTCCTGATTC CCTGGTTTCT 
CAGAATGAGA GTGAAGAAGA TGACAATCTA AATCCAACAG AAAAGCTTCA GAAACTAAAT 
GAAGTTCGTA AGAGGCTGAA TGAGTTACGT GAGCTAGTTC ACTACTATGA GCAAACATCT 
GATATGATGA CAGATTCTGT GAATGAAAAC ACTAAGGAAG AAGAAGAAAC CGAAGAATCA 
GAAAGTGATT CTGAACGTGA GGATCCACAG CCTGTTACAA ATATTAGAAA TCCTCAAGGA 
ATCAGTAGTT GGAGTGACAT AAATAGCAAC TCAAATGTAC AGTGTGGAAC TAATAACAGA 
GATGGAAGAC ATCTTAATAC AGACTGTGAA ATAAATAACA GATCTGCTGC TAACATAAGG 
ACTCTAAAAA TGTCGTCACC TTTAGACTGT CATAATAGGG AGGATGACAA AGATATTGGC 
CTACCCCAAT GTGAAGATGA AGAAGTGGAA GAAGATAGAG TTAGTGAAGA TTCTGCATCT 
AGTCACAGAA GCAGCCTGGG TGATACTGCT GGAGATGCTG AGTTTGAGCA GAAGATCAAT 
AGGCTTATAG CTGCAAAACA GAAACTTAGA CAATTACAAA ACCTTGCTGC TATGGTGCAG 
GATGATGATC CAGCACCTCA GACAACAATT GCAAATGCAT CTAATATTGG TGACTTGTTT 
TTGGGTGAGA TGGAAGAGAC AAAGCAGCAG CCAAACAATG TCCGAGCTAG TACCAACAAA 
TTACAAAAAG ATGTAGAACT GAATGAAAAA GCAAGAGAGA AGTTTTATGA AGCTAAACTC 
CAGCAGCAGC AACAGGAGCT TAAGCAGTTA CAAGAAGAAA GAAGAAAACT GATTGAAATT 
CAAGAAAAAA TTCAGGTGTT ACAGAAAGCT TGTCCTGATC TTCAATTGTC AGCTGGCTTG 
GGTGACTGCA CAGCAAATAG ACAGACTTCA CCAGCAACAT CAACTCCAGC CATGAATGAC 
ACAGCTGGCA AGCCTTCACT TGAATTTGAT GAATCTGTAC CAGTAGGCAA TGAGTTATGG 
TCTGAGATGA GAAGACATGA GATTTTAAGA GAAGAATTGC GACAAAGAAG AAAGCAACTG 
GAAGCTCTAA TGGCTGAGCA TCAGAGGAGG AAAGAGCTCG CAGAAACAAT ATCTACTGTT 
GCTGCGTCTG TTAAAAGTGA AGGATCAGAA GCTCGGCGTA CTCCACAGCA GAGTAGGACT 
GAAAAGACAA TGGCTACCTG GGGAGGTTCT ACGCAGTGTG CACTAGATGA AGAGGATGGA 
GATGAAGATG GTTATCTCTC TGATGGAGCT GGTCAGGCAG AAGAAGATGA TGATGATGCG 
TCAAGTGCGA ATGACAGCTT CTCTGTTTAT CCCAATAACA ACGTACCAGA AAATGCATAT 
TTTGTTAAAG AAAACAAGGA TAGGTGGAAA AATTACCGTC CTCTTTCAGC AGATGGAAAT 
TATCGTCCAT TGTCTAAGGC CAGACAACAG CAAAACATAA GTATGCGACG TCAGGAAAAC 
CTTCGGTGGA TGTCTGAACT TTCATATGTG GAAGAAAAGG AACGATGGCA AGAGCAGATC 
AATCAGTTGA AGAAACAGCA TGAATTTAGT GTCAGCATTT GTCAAACTTT AATGCAGGAT 
CAGCAGACCC TCTCTTGTCT TCTACAGACT TTGCTTACAG GCCCTTACAG TATGATGCCC 
AATAATGTTG CATCTTCACA AGTACATCTT ATTATGCATC AGCTAAACCA GTGTTACACT 
CAACTGACAT GGCAGCAGAA TAACGTCCAG AGGTTGAAAC AAATGTTAAA TGATCTTGTG 
CGTCAGCAAG AAGAACAACG GTATGAAGAG AAGTCATCAA GAAAGGATAG AGGCAGTGGT 
GTACCCCCAC CTCCATCTCC TGTTTTCTGT CCGTTCAGCT TTCCTCCACA ACCTTTGAAC 
CTGTTTAATG TACCAGGATT TACTAATTTT TCTTCCTTTG CTCCAGATAT TAACTATAAT 
CCAGTGTTTC CTTCTGGTTT TGGAGATTTT GCACACAATA TTTCTCCACA CAGCAGTGAG 
CAGCAGCAGC AACATCCTCT AGATCATAAT GCATCTGGGA AAACTGAGTA TATGGCGTTC 
CCCAAACCTT TTGAAAGCAG TTCCTCTAAT GGAGCGGAAA AACAAAGGAG GAGTCACAGA 
CAACCTGAAG AGGAAATGGA AAAAAGATCA GCTTGGCTTA ATGATAGCCA GGACGTGAAA 
AAAGATGATC AGTCTCAGCT GAAAGCAAGT TTTGCTGTTT CAGTACAAAA CATTGCTTCC 
AATCATAAAA ATCAGTCTGA TATGAGCAGA AGAAGAGAGT TTGATGAAGA GTCTTTGGAG 
AGTTTTAGTA GCATGCCTGA TCCAGTAGAC CCCACTACTG TGACAAAGAC ATTTAAATCT 
AGAAAAGCAT CAGCACAAGC AAGTTTGGCA TCAAAAGATA AAACACCTAA ATCGAAGAAT 
AAGAGGAAGA GTTCTTCTCA GCTAAAAGGC AGAATTACAA ATATTGATTA TGAAAGTGCC 
AGTGCTTCTA GTGTGTGTGA ACCCTGCAAG AGCAATAAAA GCAGACACTC TGAAGAGGTT 
GGTCATGCAA AGGTGTTCAG CAAAAGGAAT CGGGAACAAC TGGAAAAAAT CATTAAATAC 
AGTAGATCTA CAGAAATGTC TTCAGCGCAT GCTAGGAGAA TTCTGCAGCA GTCTAACAGA 
AATGCATGCA TTGAAGCGCC AGAAACTGGT AGTGATCTTT CTATGTTTGA AGCTTTACGA 
GACACAATTT ATTCTGAAGT GGCAACTCTT ATTTCTCAAA ATGAGTCTCG TCCCCACTTT 
CTTATTGAAC TTTTCCATGA GCTTCAGCTG CTAAATACAG ATTACCTGAG GCAAAGAGCT 
CTATACGCTC TACAGGATAT AGTGACCAGA CATTTATCTG AGAACAATGA AAAAGGGAAG 
TGCACAAAAT CACTGAATTC TGCAGCATGG ATAGCATCAA ATTCTGAACT CACTCCCAGT 
GAAAGCCTTG CTTCTACAGA TGATGAAACT TTTGGCAAAA ACTTTTCTAC AGAAGCATGT 
CAAGATTGTG AACAAAATGA TGCAGACAAT GGGAGTAGCA TGTCTACATC TTCAAATTTT 
GAACCCTTTG CCACTGATGA CCTTGGCAAC ACAGTGATTC ACTTAGATCA AGCTTTGGCT 
AGGATGAGGG AGTATGAGCG TATGAAAATT GAAGCTGAAA GTACCCTTGA CTCTGAGGGC 
TGCTCTAGTA ATTTTCAGGG TGCTTCTGCT GCTAAATTAG AAGGAGGTCA AAGTGCCAGT 
GAATGTGTTC CTGTGCCACA GTCAAGTGAA GCTTCTGCTG TTCCATGTCC TCATATAGAC 
ACTCAGCAGC TTGACCGGCA GATTAAAGCA ATTATGAAAG AGGTCATTCC TTTTCTGAAG 
GAACACATGG ATGAAGTATG CTCTTCGCAG TTACTGACAT CAGTAAGACG TATGGTCTTG 
ACCCTTACAC AACAAAATGA TGAAAGTAAA GAGTTTGTGA AGTTCTTTCA TAAGCAGCTT 
GGCAGTATAC TTCAGGATTC ACTGGCAAAA TTTGCTGGTA GAAAACTGAA AGATTGTGGA 
GAGGATCTTC TAGTGGAGAT CTCTGAAGTG TTATTTAATG AATTAGCCTT TTTTAAACTC 
ATGCAAGACT TGGACAACAA CAGTATTTCT GTAAAGCAGA GATGTAAAAG AAAAATAGAA 
GCTACTGAAG TGATACAGTC GTATGCTAAA GAGGCAAAAA AAGGTCTCCA GGTGGATGTT 
TGTTCATCTG CTGAAGATGT CGATGAGGAC AAAGACAAGG ATGAGACTGA AACTGTTAAA 
CAAGTACAGG ACTCAGAAAT GTATGATGGG AATAGAGTTC CTGAAAGTAT TAGGTCTGAT 
GCATCTGATC AGGAGGAAGA TGATGAAAGT GAAAGCGGTC CAGTGGCAAT AAGTTTATCA 
AAAGCAGAAA CCCAAGCTCT GACTAACTAT GGTAGTGGAG AAGATGAGAA TGAAGATGAA 
GAAATAGAAT TTGAGGAAGG ACCTGTTGAT GTGCAGACGT CATTACAAGC CAGCAGTGAA 
ACAACAACTG AAAATGAACA GAATTCAAAC CAAGAACAAA GTAAGACAAA AAGCAGTGAG 
ATTCTGTCAT CAGAACAAGA ATCTGTTTAT GCTAAAGGTG AACAACATGT GGCTACAATT 
TTGCCTTATT ACCTCAATGT AATGGAGAAT GCACCGCCTT TAACGGTCAA TACCCCAGAA 
TCTTTTATAA CAGACAGTAT GAAAACAGAA GAATCAAGTT CATCTTTACC AGTAAATGAA 
ACTCAAACAC TGGATACAAC GTGTGTAGGA AACAAATCTG CTGCAAGTTC TGAAAGCTCC 
ATGGCTGGCA GCCCTGATAC AGAGTCACCT GTGTTAGTGA ATGAATATGA AGCTGGTTCT 
GGAAATGTAA GTCAAAGATC TGATGAAGAT GACTTTGTGA AAGTTGAAGA CTTGCCCCTT 
AAACTTGCAG TGTATTCAGA GGCAGATTTA ATGAAGAAAA TGGCAATGGA GGCCCAAACG 
AACAGTTTGT CTGATGAATT ACTGGGTGGA GGTGGAGCTC AAGATCAAGA ATTAGTAGGA 
GATGCCCAAA CTTTGAAAGA ACCTGAAACT TTTGGAGTTC AAAGTGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021141541.1
CDS	140..6250
Translation	MATGGGPFEEGMNDQDLPSWSNDSFDDRLNNTDWGSQQKKANRSSEKNKKKLGGEAETRLTNDISPESSPGIGRRKTRTPHSFPHARYMTQMSVPEQAELERLKQRINFSDLDQRSIGSDSQGRATAANNKRQLNENKKPFNFLSLQINTNKSKDPASCSQKKESGVSVQRKELFGAALSKDFLQNCQVSAQEDGGGEPAMDSSQIVSRLVQIRDYIAKASSMRDDLVEKNERSANVERLSHLIDHLKEQEKSYLKFLQKMLARDPQQEAKEELENLKKQHDLLKRMLKQQEQLKALQGRQAALLALQHKAEQAIAVMDDSVVTETTGSVSGVSLTSELNEELNDLIQRFHNQLHDSQTQSVPDNRRQAESLSLTREVSQSRNSSMSERLSDEKAQLFNKMRMLQGKKQKMDKLLGELHTLRDQHLNNSSFFPASGSPQRSIDQRSTTSAASGPAGIVTVVNGESNSLASAPYPPDSLVSQNESEEDDNLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDSVNENTKEEEETEESESDSEREDPQPVTNIRNPQGISSWSDINSNSNVQCGTNNRDGRHLNTDCEINNRSAANIRTLKMSSPLDCHNREDDKDIGLPQCEDEEVEEDRVSEDSASSHRSSLGDTAGDAEFEQKINRLIAAKQKLRQLQNLAAMVQDDDPAPQTTIANASNIGDLFLGEMEETKQQPNNVRASTNKLQKDVELNEKAREKFYEAKLQQQQQELKQLQEERRKLIEIQEKIQVLQKACPDLQLSAGLGDCTANRQTSPATSTPAMNDTAGKPSLEFDESVPVGNELWSEMRRHEILREELRQRRKQLEALMAEHQRRKELAETISTVAASVKSEGSEARRTPQQSRTEKTMATWGGSTQCALDEEDGDEDGYLSDGAGQAEEDDDDASSANDSFSVYPNNNVPENAYFVKENKDRWKNYRPLSADGNYRPLSKARQQQNISMRRQENLRWMSELSYVEEKERWQEQINQLKKQHEFSVSICQTLMQDQQTLSCLLQTLLTGPYSMMPNNVASSQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNDLVRQQEEQRYEEKSSRKDRGSGVPPPPSPVFCPFSFPPQPLNLFNVPGFTNFSSFAPDINYNPVFPSGFGDFAHNISPHSSEQQQQHPLDHNASGKTEYMAFPKPFESSSSNGAEKQRRSHRQPEEEMEKRSAWLNDSQDVKKDDQSQLKASFAVSVQNIASNHKNQSDMSRRREFDEESLESFSSMPDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKNKRKSSSQLKGRITNIDYESASASSVCEPCKSNKSRHSEEVGHAKVFSKRNREQLEKIIKYSRSTEMSSAHARRILQQSNRNACIEAPETGSDLSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHLSENNEKGKCTKSLNSAAWIASNSELTPSESLASTDDETFGKNFSTEACQDCEQNDADNGSSMSTSSNFEPFATDDLGNTVIHLDQALARMREYERMKIEAESTLDSEGCSSNFQGASAAKLEGGQSASECVPVPQSSEASAVPCPHIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSISVKQRCKRKIEATEVIQSYAKEAKKGLQVDVCSSAEDVDEDKDKDETETVKQVQDSEMYDGNRVPESIRSDASDQEEDDESESGPVAISLSKAETQALTNYGSGEDENEDEEIEFEEGPVDVQTSLQASSETTTENEQNSNQEQSKTKSSEILSSEQESVYAKGEQHVATILPYYLNVMENAPPLTVNTPESFITDSMKTEESSSSLPVNETQTLDTTCVGNKSAASSESSMAGSPDTESPVLVNEYEAGSGNVSQRSDEDDFVKVEDLPLKLAVYSEADLMKKMAMEAQTNSLSDELLGGGGAQDQELVGDAQTLKEPETFGVQSA

Target ORF information:

RefSeq Version	XM_021285866.1
Organism	Columba livia(rock pigeon)
Definition	Columba livia pericentriolar material 1 (PCM1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021285866.1

ORF Insert Sequence:

ATGGCAACAG GAGGTGGTCC CTTTGAAGAA GGCATGAATG ATCAAGACTT GCCTAGCTGG 
AGCAATGACA GCTTTGATGA CCGGCTGAAC AACACGGACT GGGGAAGTCA ACAGAAGAAA 
GCAAACAGAT CTTCAGAAAA AAACAAGAAA AAGCTTGGTG GGGAAGCTGA AACAAGGCTT 
ACTAATGATA TATCTCCAGA ATCCTCACCT GGAATAGGAC GACGAAAGAC CAGAACTCCT 
CATAGTTTTC CTCATGCTCG ATACATGACC CAGATGTCTG TTCCGGAGCA GGCTGAGCTA 
GAAAGGCTTA AACAAAGAAT AAACTTCAGT GATCTGGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGCA GGGCAACAGC TGCTAACAAC AAACGTCAAC TTAATGAAAA CAAAAAACCA 
TTCAACTTCC TGTCACTGCA GATAAACACT AACAAGAGCA AAGATCCTGC CTCATGTTCC 
CAAAAAAAGG AAAGTGGGGT GTCAGTCCAA CGTAAAGAGT TGTTTGGTGC TGCTCTGAGC 
AAGGATTTCT TGCAAAATTG TCAAGTCTCT GCTCAAGAAG ATGGAGGGGG AGAACCAGCT 
ATGGATAGTA GCCAGATTGT GAGCAGACTA GTTCAAATTC GCGACTATAT TGCTAAGGCC 
AGCTCCATGC GGGATGATCT TGTAGAGAAA AATGAAAGAT CGGCCAATGT TGAGCGTTTA 
TCACACCTTA TAGATCACCT TAAAGAGCAG GAGAAATCCT ATCTGAAATT TTTGCAAAAG 
ATGCTTGCTA GAGATCCTCA ACAGGAGGCT AAAGAGGAGT TGGAGAACTT GAAGAAGCAG 
CATGATTTAT TGAAAAGGAT GCTAAAACAG CAAGAGCAAT TAAAGGCTCT TCAAGGAAGA 
CAGGCAGCTC TTCTTGCTTT GCAGCATAAA GCAGAGCAAG CAATTGCTGT CATGGATGAT 
TCTGTTGTAA CAGAGACTAC AGGTAGTGTT TCAGGAGTAA GCCTTACATC AGAACTGAAT 
GAAGAATTGA ATGACTTAAT TCAGCGCTTT CACAACCAAC TTCATGATTC TCAGACACAG 
TCTGTACCAG ACAATAGAAG GCAAGCAGAA AGTCTTTCAC TTACTAGAGA GGTTTCACAA 
AGCAGAAACT CTTCAATGTC TGAACGCCTG TCAGATGAGA AGGCGCAGCT TTTTAACAAG 
ATGCGAATGT TGCAGGGTAA AAAGCAAAAA ATGGACAAAC TGTTAGGAGA ACTTCATACA 
CTTCGTGACC AACATCTAAA TAACTCCTCC TTTTTTCCTG CTTCAGGTTC TCCTCAAAGA 
AGTATTGATC AAAGAAGTAC AACTTCAGCT GCTTCTGGTC CTGCAGGCAT AGTAACTGTT 
GTCAATGGCG AATCAAATAG CCTGGCATCT GCTCCCTATC CTCCTGATTC CCTGGTTTCT 
CAGAATGAGA GTGAAGAAGA TGACAATCTA AATCCAACAG AAAAGCTTCA GAAACTAAAT 
GAAGTTCGTA AGAGGCTGAA TGAGTTACGT GAGCTAGTTC ACTACTATGA GCAAACATCT 
GATATGATGA CAGATTCTGT GAATGAAAAC ACTAAGGAAG AAGAAGAAAC CGAAGAATCA 
GAAAGTGATT CTGAACGTGA GGATCCACAG CCTGTTACAA ATATTAGAAA TCCTCAAGGA 
ATCAGTAGTT GGAGTGACAT AAATAGCAAC TCAAATGTAC AGTGTGGAAC TAATAACAGA 
GATGGAAGAC ATCTTAATAC AGACTGTGAA ATAAATAACA GATCTGCTGC TAACATAAGG 
ACTCTAAAAA TGTCGTCACC TTTAGACTGT CATAATAGGG AGGATGACAA AGATATTGGC 
CTACCCCAAT GTGAAGATGA AGAAGTGGAA GAAGATAGAG TTAGTGAAGA TTCTGCATCT 
AGTCACAGAA GCAGCCTGGG TGATACTGCT GGAGATGCTG AGTTTGAGCA GAAGATCAAT 
AGGCTTATAG CTGCAAAACA GAAACTTAGA CAATTACAAA ACCTTGCTGC TATGGTGCAG 
GATGATGATC CAGCACCTCA GACAACAATT GCAAATGCAT CTAATATTGG TGACTTGTTT 
TTGGGTGAGA TGGAAGAGAC AAAGCAGCAG CCAAACAATG TCCGAGCTAG TACCAACAAA 
TTACAAAAAG ATGTAGAACT GAATGAAAAA GCAAGAGAGA AGTTTTATGA AGCTAAACTC 
CAGCAGCAGC AACAGGAGCT TAAGCAGTTA CAAGAAGAAA GAAGAAAACT GATTGAAATT 
CAAGAAAAAA TTCAGGTGTT ACAGAAAGCT TGTCCTGATC TTCAATTGTC AGCTGGCTTG 
GGTGACTGCA CAGCAAATAG ACAGACTTCA CCAGCAACAT CAACTCCAGC CATGAATGAC 
ACAGCTGGCA AGCCTTCACT TGAATTTGAT GAATCTGTAC CAGTAGGCAA TGAGTTATGG 
TCTGAGATGA GAAGACATGA GATTTTAAGA GAAGAATTGC GACAAAGAAG AAAGCAACTG 
GAAGCTCTAA TGGCTGAGCA TCAGAGGAGG AAAGAGCTCG CAGAAACAAT ATCTACTGTT 
GCTGCGTCTG TTAAAAGTGA AGGATCAGAA GCTCGGCGTA CTCCACAGCA GAGTAGGACT 
GAAAAGACAA TGGCTACCTG GGGAGGTTCT ACGCAGTGTG CACTAGATGA AGAGGATGGA 
GATGAAGATG GTTATCTCTC TGATGGAGCT GGTCAGGCAG AAGAAGATGA TGATGATGCG 
TCAAGTGCGA ATGACAGCTT CTCTGTTTAT CCCAATAACA ACGTACCAGA AAATGCATAT 
TTTGTTAAAG AAAACAAGGA TAGGTGGAAA AATTACCGTC CTCTTTCAGC AGATGGAAAT 
TATCGTCCAT TGTCTAAGGC CAGACAACAG CAAAACATAA GTATGCGACG TCAGGAAAAC 
CTTCGGTGGA TGTCTGAACT TTCATATGTG GAAGAAAAGG AACGATGGCA AGAGCAGATC 
AATCAGTTGA AGAAACAGCA TGAATTTAGT GTCAGCATTT GTCAAACTTT AATGCAGGAT 
CAGCAGACCC TCTCTTGTCT TCTACAGACT TTGCTTACAG GCCCTTACAG TATGATGCCC 
AATAATGTTG CATCTTCACA AGTACATCTT ATTATGCATC AGCTAAACCA GTGTTACACT 
CAACTGACAT GGCAGCAGAA TAACGTCCAG AGGTTGAAAC AAATGTTAAA TGATCTTGTG 
CGTCAGCAAG AAGAACAACG GTATGAAGAG AAGTCATCAA GAAAGGATAG AGGCAGTGGT 
GTACCCCCAC CTCCATCTCC TGTTTTCTGT CCGTTCAGCT TTCCTCCACA ACCTTTGAAC 
CTGTTTAATG TACCAGGATT TACTAATTTT TCTTCCTTTG CTCCAGATAT TAACTATAAT 
CCAGTGTTTC CTTCTGGTTT TGGAGATTTT GCACACAATA TTTCTCCACA CAGCAGTGAG 
CAGCAGCAGC AACATCCTCT AGATCATAAT GCATCTGGGA AAACTGAGTA TATGGCGTTC 
CCCAAACCTT TTGAAAGCAG TTCCTCTAAT GGAGCGGAAA AACAAAGGAG GAGTCACAGA 
CAACCTGAAG AGGAAATGGA AAAAAGATCA GCTTGGCTTA ATGATAGCCA GGACGTGAAA 
AAAGATGATC AGTCTCAGCT GAAAGCAAGT TTTGCTGTTT CAGTACAAAA CATTGCTTCC 
AATCATAAAA ATCAGTCTGA TATGAGCAGA AGAAGAGAGT TTGATGAAGA GTCTTTGGAG 
AGTTTTAGTA GCATGCCTGA TCCAGTAGAC CCCACTACTG TGACAAAGAC ATTTAAATCT 
AGAAAAGCAT CAGCACAAGC AAGTTTGGCA TCAAAAGATA AAACACCTAA ATCGAAGAAT 
AAGAGGAAGA GTTCTTCTCA GCTAAAAGGC AGAATTACAA ATATTGATTA TGAAAGTGCC 
AGTGCTTCTA GTGTGTGTGA ACCCTGCAAG AGCAATAAAA GCAGACACTC TGAAGAGGTT 
GGTCATGCAA AGGTGTTCAG CAAAAGGAAT CGGGAACAAC TGGAAAAAAT CATTAAATAC 
AGTAGATCTA CAGAAATGTC TTCAGCGCAT GCTAGGAGAA TTCTGCAGCA GTCTAACAGA 
AATGCATGCA TTGAAGCGCC AGAAACTGGT AGTGATCTTT CTATGTTTGA AGCTTTACGA 
GACACAATTT ATTCTGAAGT GGCAACTCTT ATTTCTCAAA ATGAGTCTCG TCCCCACTTT 
CTTATTGAAC TTTTCCATGA GCTTCAGCTG CTAAATACAG ATTACCTGAG GCAAAGAGCT 
CTATACGCTC TACAGGATAT AGTGACCAGA CATTTATCTG AGAACAATGA AAAAGGGAAG 
TGCACAAAAT CACTGAATTC TGCAGCATGG ATAGCATCAA ATTCTGAACT CACTCCCAGT 
GAAAGCCTTG CTTCTACAGA TGATGAAACT TTTGGCAAAA ACTTTTCTAC AGAAGCATGT 
CAAGATTGTG AACAAAATGA TGCAGACAAT GGGAGTAGCA TGTCTACATC TTCAAATTTT 
GAACCCTTTG CCACTGATGA CCTTGGCAAC ACAGTGATTC ACTTAGATCA AGCTTTGGCT 
AGGATGAGGG AGTATGAGCG TATGAAAATT GAAGCTGAAA GTACCCTTGA CTCTGAGGGC 
TGCTCTAGTA ATTTTCAGGG TGCTTCTGCT GCTAAATTAG AAGGAGGTCA AAGTGCCAGT 
GAATGTGTTC CTGTGCCACA GTCAAGTGAA GCTTCTGCTG TTCCATGTCC TCATATAGAC 
ACTCAGCAGC TTGACCGGCA GATTAAAGCA ATTATGAAAG AGGTCATTCC TTTTCTGAAG 
GAACACATGG ATGAAGTATG CTCTTCGCAG TTACTGACAT CAGTAAGACG TATGGTCTTG 
ACCCTTACAC AACAAAATGA TGAAAGTAAA GAGTTTGTGA AGTTCTTTCA TAAGCAGCTT 
GGCAGTATAC TTCAGGATTC ACTGGCAAAA TTTGCTGGTA GAAAACTGAA AGATTGTGGA 
GAGGATCTTC TAGTGGAGAT CTCTGAAGTG TTATTTAATG AATTAGCCTT TTTTAAACTC 
ATGCAAGACT TGGACAACAA CAGTATTTCT GTAAAGCAGA GATGTAAAAG AAAAATAGAA 
GCTACTGAAG TGATACAGTC GTATGCTAAA GAGGCAAAAA AAGGTCTCCA GGTGGATGTT 
TGTTCATCTG CTGAAGATGT CGATGAGGAC AAAGACAAGG ATGAGACTGA AACTGTTAAA 
CAAGTACAGG ACTCAGAAAT GTATGATGGG AATAGAGTTC CTGAAAGTAT TAGGTCTGAT 
GCATCTGATC AGGAGGAAGA TGATGAAAGT GAAAGCGGTC CAGTGGCAAT AAGTTTATCA 
AAAGCAGAAA CCCAAGCTCT GACTAACTAT GGTAGTGGAG AAGATGAGAA TGAAGATGAA 
GAAATAGAAT TTGAGGAAGG ACCTGTTGAT GTGCAGACGT CATTACAAGC CAGCAGTGAA 
ACAACAACTG AAAATGAACA GAATTCAAAC CAAGAACAAA GTAAGACAAA AAGCAGTGAG 
ATTCTGTCAT CAGAACAAGA ATCTGTTTAT GCTAAAGGTG AACAACATGT GGCTACAATT 
TTGCCTTATT ACCTCAATGT AATGGAGAAT GCACCGCCTT TAACGGTCAA TACCCCAGAA 
TCTTTTATAA CAGACAGTAT GAAAACAGAA GAATCAAGTT CATCTTTACC AGTAAATGAA 
ACTCAAACAC TGGATACAAC GTGTGTAGGA AACAAATCTG CTGCAAGTTC TGAAAGCTCC 
ATGGCTGGCA GCCCTGATAC AGAGTCACCT GTGTTAGTGA ATGAATATGA AGCTGGTTCT 
GGAAATGTAA GTCAAAGATC TGATGAAGAT GACTTTGTGA AAGTTGAAGA CTTGCCCCTT 
AAACTTGCAG TGTATTCAGA GGCAGATTTA ATGAAGAAAA TGGCAATGGA GGCCCAAACG 
AACAGTTTGT CTGATGAATT ACTGGGTGGA GGTGGAGCTC AAGATCAAGA ATTAGTAGGA 
GATGCCCAAA CTTTGAAAGA ACCTGAAACT TTTGGAGTTC AAAGTGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_021285867.1 ) cDNA ORF clone, Columba livia(rock pigeon) -> XP_021141542.1 Columba livia pericentriolar material 1 (PCM1), transcript variant X14, mRNA.

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CloneID	OCo293170
Clone ID Related Accession (Same CDS sequence)	XM_021285867.1
Accession Version	XM_021285867.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6060bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-23
Organism	Columba livia(rock pigeon)
Product	pericentriolar material 1 protein isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004973253.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Columba livia Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACAG GAGGTGGTCC CTTTGAAGAA GGCATGAATG ATCAAGACTT GCCTAGCTGG 
AGCAATGACA GCTTTGATGA CCGGCTGAAC AACACGGACT GGGGAAGTCA ACAGAAGAAA 
GCAAACAGAT CTTCAGAAAA AAACAAGAAA AAGCTTGGTG GGGAAGCTGA AACAAGGCTT 
ACTAATGATA TATCTCCAGA ATCCTCACCT GGAATAGGAC GACGAAAGAC CAGAACTCCT 
CATAGTTTTC CTCATGCTCG ATACATGACC CAGATGTCTG TTCCGGAGCA GGCTGAGCTA 
GAAAGGCTTA AACAAAGAAT AAACTTCAGT GATCTGGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGCA GGGCAACAGC TGCTAACAAC AAACGTCAAC TTAATGAAAA CAAAAAACCA 
TTCAACTTCC TGTCACTGCA GATAAACACT AACAAGAGCA AAGATCCTGC CTCATGTTCC 
CAAAAAAAGG AAAGTGGGGT GTCAGTCCAA CGTAAAGAGT TGTTTGGTGC TGCTCTGAGC 
AAGGATTTCT TGCAAAATTG TCAAGTCTCT GCTCAAGAAG ATGGAGGGGG AGAACCAGCT 
ATGGATAGTA GCCAGGCTAG ACAAAATGAG GAGGATGATG TTCGGACTAT AGATTCAGCT 
GTGGGATCTG GTTCTGTAGG TGAGAGCACA TCGCTAAACA TTGATGTGCA GTCTGAGGCT 
TCAGATACCA CGGCTAGAGA TCCTCAACAG GAGGCTAAAG AGGAGTTGGA GAACTTGAAG 
AAGCAGCATG ATTTATTGAA AAGGATGCTA AAACAGCAAG AGCAATTAAA GGCTCTTCAA 
GGAAGACAGG CAGCTCTTCT TGCTTTGCAG CATAAAGCAG AGCAAGCAAT TGCTGTCATG 
GATGATTCTG TTGTAACAGA GACTACAGGT AGTGTTTCAG GAGTAAGCCT TACATCAGAA 
CTGAATGAAG AATTGAATGA CTTAATTCAG CGCTTTCACA ACCAACTTCA TGATTCTCAG 
ACACAGTCTG TACCAGACAA TAGAAGGCAA GCAGAAAGTC TTTCACTTAC TAGAGAGGTT 
TCACAAAGCA GAAACTCTTC AATGTCTGAA CGCCTGTCAG ATGAGAAGGC GCAGCTTTTT 
AACAAGATGC GAATGTTGCA GGGTAAAAAG CAAAAAATGG ACAAACTGTT AGGAGAACTT 
CATACACTTC GTGACCAACA TCTAAATAAC TCCTCCTTTT TTCCTGCTTC AGGTTCTCCT 
CAAAGAAGTA TTGATCAAAG AAGTACAACT TCAGCTGCTT CTGGTCCTGC AGGCATAGTA 
ACTGTTGTCA ATGGCGAATC AAATAGCCTG GCATCTGCTC CCTATCCTCC TGATTCCCTG 
GTTTCTCAGA ATGAGAGTGA AGAAGATGAC AATCTAAATC CAACAGAAAA GCTTCAGAAA 
CTAAATGAAG TTCGTAAGAG GCTGAATGAG TTACGTGAGC TAGTTCACTA CTATGAGCAA 
ACATCTGATA TGATGACAGA TTCTGTGAAT GAAAACACTA AGGAAGAAGA AGAAACCGAA 
GAATCAGAAA GTGATTCTGA ACGTGAGGAT CCACAGCCTG TTACAAATAT TAGAAATCCT 
CAAGGAATCA GTAGTTGGAG TGACATAAAT AGCAACTCAA ATGTACAGTG TGGAACTAAT 
AACAGAGATG GAAGACATCT TAATACAGAC TGTGAAATAA ATAACAGATC TGCTGCTAAC 
ATAAGGACTC TAAAAATGTC GTCACCTTTA GACTGTCATA ATAGGGAGGA TGACAAAGAT 
ATTGGCCTAC CCCAATGTGA AGATGAAGAA GTGGAAGAAG ATAGAGTTAG TGAAGATTCT 
GCATCTAGTC ACAGAAGCAG CCTGGGTGAT ACTGCTGGAG ATGCTGAGTT TGAGCAGAAG 
ATCAATAGGC TTATAGCTGC AAAACAGAAA CTTAGACAAT TACAAAACCT TGCTGCTATG 
GTGCAGGATG ATGATCCAGC ACCTCAGACA ACAATTGCAA ATGCATCTAA TATTGGTGAC 
TTGTTTTTGG GTGAGATGGA AGAGACAAAG CAGCAGCCAA ACAATGTCCG AGCTAGTACC 
AACAAATTAC AAAAAGATGT AGAACTGAAT GAAAAAGCAA GAGAGAAGTT TTATGAAGCT 
AAACTCCAGC AGCAGCAACA GGAGCTTAAG CAGTTACAAG AAGAAAGAAG AAAACTGATT 
GAAATTCAAG AAAAAATTCA GGTGTTACAG AAAGCTTGTC CTGATCTTCA ATTGTCAGCT 
GGCTTGGGTG ACTGCACAGC AAATAGACAG ACTTCACCAG CAACATCAAC TCCAGCCATG 
AATGACACAG CTGGCAAGCC TTCACTTGAA TTTGATGAAT CTGTACCAGT AGGCAATGAG 
TTATGGTCTG AGATGAGAAG ACATGAGATT TTAAGAGAAG AATTGCGACA AAGAAGAAAG 
CAACTGGAAG CTCTAATGGC TGAGCATCAG AGGAGGAAAG AGCTCGCAGA AACAATATCT 
ACTGTTGCTG CGTCTGTTAA AAGTGAAGGA TCAGAAGCTC GGCGTACTCC ACAGCAGAGT 
AGGACTGAAA ATAGGACAAT GGCTACCTGG GGAGGTTCTA CGCAGTGTGC ACTAGATGAA 
GAGGATGGAG ATGAAGATGG TTATCTCTCT GATGGAGCTG GTCAGGCAGA AGAAGATGAT 
GATGATGCGT CAAGTGCGAA TGACAGCTTC TCTGTTTATC CCAATAACAA CGTACCAGAA 
AATGCATATT TTGTTAAAGA AAACAAGGAT AGGTGGAAAA ATTACCGTCC TCTTTCAGCA 
GATGGAAATT ATCGTCCATT GTCTAAGGCC AGACAACAGC AAAACATAAG TATGCGACGT 
CAGGAAAACC TTCGGTGGAT GTCTGAACTT TCATATGTGG AAGAAAAGGA ACGATGGCAA 
GAGCAGATCA ATCAGTTGAA GAAACAGCAT GAATTTAGTG TCAGCATTTG TCAAACTTTA 
ATGCAGGATC AGCAGACCCT CTCTTGTCTT CTACAGACTT TGCTTACAGG CCCTTACAGT 
ATGATGCCCA ATAATGTTGC ATCTTCACAA GTACATCTTA TTATGCATCA GCTAAACCAG 
TGTTACACTC AACTGACATG GCAGCAGAAT AACGTCCAGA GGTTGAAACA AATGTTAAAT 
GATCTTGTGC GTCAGCAAGA AGAACAACGG TATGAAGAGA AGTCATCAAG AAAGGATAGA 
GGCAGTGGTG TACCCCCACC TCCATCTCCT GTTTTCTGTC CGTTCAGCTT TCCTCCACAA 
CCTTTGAACC TGTTTAATGT ACCAGGATTT ACTAATTTTT CTTCCTTTGC TCCAGATATT 
AACTATAATC CAGTGTTTCC TTCTGGTTTT GGAGATTTTG CACACAATAT TTCTCCACAC 
AGCAGTGAGC AGCAGCAGCA ACATCCTCTA GATCATAATG CATCTGGGAA AACTGAGTAT 
ATGGCGTTCC CCAAACCTTT TGAAAGCAGT TCCTCTAATG GAGCGGAAAA ACAAAGGAGG 
AGTCACAGAC AACCTGAAGA GGAAATGGAA AAAAGATCAG CTTGGCTTAA TGATAGCCAG 
GACGTGAAAA AAGATGATCA GTCTCAGCTG AAAGCAAGTT TTGCTGTTTC AGTACAAAAC 
ATTGCTTCCA ATCATAAAAA TCAGTCTGAT ATGAGCAGAA GAAGAGAGTT TGATGAAGAG 
TCTTTGGAGA GTTTTAGTAG CATGCCTGAT CCAGTAGACC CCACTACTGT GACAAAGACA 
TTTAAATCTA GAAAAGCATC AGCACAAGCA AGTTTGGCAT CAAAAGATAA AACACCTAAA 
TCGAAGAATA AGAGGAAGAG TTCTTCTCAG CTAAAAGGCA GAATTACAAA TATTGATTAT 
GAAAGTGCCA GTGCTTCTAG TGTGTGTGAA CCCTGCAAGA GCAATAAAAG CAGACACTCT 
GAAGAGGTTG GTCATGCAAA GGTGTTCAGC AAAAGGAATC GGGAACAACT GGAAAAAATC 
ATTAAATACA GTAGATCTAC AGAAATGTCT TCAGCGCATG CTAGGAGAAT TCTGCAGCAG 
TCTAACAGAA ATGCATGCAT TGAAGCGCCA GAAACTGGTA GTGATCTTTC TATGTTTGAA 
GCTTTACGAG ACACAATTTA TTCTGAAGTG GCAACTCTTA TTTCTCAAAA TGAGTCTCGT 
CCCCACTTTC TTATTGAACT TTTCCATGAG CTTCAGCTGC TAAATACAGA TTACCTGAGG 
CAAAGAGCTC TATACGCTCT ACAGGATATA GTGACCAGAC ATTTATCTGA GAACAATGAA 
AAAGGGAAGT GCACAAAATC ACTGAATTCT GCAGCATGGA TAGCATCAAA TTCTGAACTC 
ACTCCCAGTG AAAGCCTTGC TTCTACAGAT GATGAAACTT TTGGCAAAAA CTTTTCTACA 
GAAGCATGTC AAGATTGTGA ACAAAATGAT GCAGACAATG GGAGTAGCAT GTCTACATCT 
TCAAATTTTG AACCCTTTGC CACTGATGAC CTTGGCAACA CAGTGATTCA CTTAGATCAA 
GCTTTGGCTA GGATGAGGGA GTATGAGCGT ATGAAAATTG AAGCTGAAAG TACCCTTGAC 
TCTGAGGGCT GCTCTAGTAA TTTTCAGGGT GCTTCTGCTG CTAAATTAGA AGGAGGTCAA 
AGTGCCAGTG AATGTGTTCC TGTGCCACAG TCAAGTGAAG CTTCTGCTGT TCCATGTCCT 
CATATAGACA CTCAGCAGCT TGACCGGCAG ATTAAAGCAA TTATGAAAGA GGTCATTCCT 
TTTCTGAAGG AACACATGGA TGAAGTATGC TCTTCGCAGT TACTGACATC AGTAAGACGT 
ATGGTCTTGA CCCTTACACA ACAAAATGAT GAAAGTAAAG AGTTTGTGAA GTTCTTTCAT 
AAGCAGCTTG GCAGTATACT TCAGGATTCA CTGGCAAAAT TTGCTGGTAG AAAACTGAAA 
GATTGTGGAG AGGATCTTCT AGTGGAGATC TCTGAAGTGT TATTTAATGA ATTAGCCTTT 
TTTAAACTCA TGCAAGACTT GGACAACAAC AGTATTTCTG TAAAGCAGAG ATGTAAAAGA 
AAAATAGAAG CTACTGAAGT GATACAGTCG TATGCTAAAG AGGCAAAAAA AGGTCTCCAG 
GTGGATGTTT GTTCATCTGC TGAAGATGTC GATGAGGACA AAGACAAGGA TGAGACTGAA 
ACTGTTAAAC AAGTACAGGA CTCAGAAATG TATGATGGGA ATAGAGTTCC TGAAAGTATT 
AGGTCTGATG CATCTGATCA GGAGGAAGAT GATGAAAGTG AAAGCGGTCC AGTGGCAATA 
AGTTTATCAA AAGCAGAAAC CCAAGCTCTG ACTAACTATG GTAGTGGAGA AGATGAGAAT 
GAAGATGAAG AAATAGAATT TGAGGAAGGA CCTGTTGATG TGCAGACGTC ATTACAAGCC 
AGCAGTGAAA CAACAACTGA AAATGAACAG AATTCAAACC AAGAACAAAG TAAGACAAAA 
AGCAGTGAGA TTCTGTCATC AGAACAAGAA TCTGTTTATG CTAAAGGTGA ACAACATGTG 
GCTACAATTT TGCCTTATTA CCTCAATGTA ATGGAGAATG CACCGCCTTT AACGGTCAAT 
ACCCCAGAAT CTTTTATAAC AGACAGTATG AAAACAGAAG AATCAAGTTC ATCTTTACCA 
GTAAATGAAA CTCAAACACT GGATACAACG TGTGTAGGAA ACAAATCTGC TGCAAGTTCT 
GAAAGCTCCA TGGCTGGCAG CCCTGATACA GAGTCACCTG TGTTAGTGAA TGAATATGAA 
GCTGGTTCTG GAAATGTAAG TCAAAGATCT GATGAAGATG ACTTTGTGAA AGTTGAAGAC 
TTGCCCCTTA AACTTGCAGT GTATTCAGAG GCAGATTTAA TGAAGAAAAT GGCAATGGAG 
GCCCAAACGA ACAGTTTGTC TGATGAATTA CTGGGTGGAG GTGGAGCTCA AGATCAAGAA 
TTAGTAGGAG ATGCCCAAAC TTTGAAAGAA CCTGAAACTT TTGGAGTTCA AAGTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021141542.1
CDS	140..6199
Translation	MATGGGPFEEGMNDQDLPSWSNDSFDDRLNNTDWGSQQKKANRSSEKNKKKLGGEAETRLTNDISPESSPGIGRRKTRTPHSFPHARYMTQMSVPEQAELERLKQRINFSDLDQRSIGSDSQGRATAANNKRQLNENKKPFNFLSLQINTNKSKDPASCSQKKESGVSVQRKELFGAALSKDFLQNCQVSAQEDGGGEPAMDSSQARQNEEDDVRTIDSAVGSGSVGESTSLNIDVQSEASDTTARDPQQEAKEELENLKKQHDLLKRMLKQQEQLKALQGRQAALLALQHKAEQAIAVMDDSVVTETTGSVSGVSLTSELNEELNDLIQRFHNQLHDSQTQSVPDNRRQAESLSLTREVSQSRNSSMSERLSDEKAQLFNKMRMLQGKKQKMDKLLGELHTLRDQHLNNSSFFPASGSPQRSIDQRSTTSAASGPAGIVTVVNGESNSLASAPYPPDSLVSQNESEEDDNLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDSVNENTKEEEETEESESDSEREDPQPVTNIRNPQGISSWSDINSNSNVQCGTNNRDGRHLNTDCEINNRSAANIRTLKMSSPLDCHNREDDKDIGLPQCEDEEVEEDRVSEDSASSHRSSLGDTAGDAEFEQKINRLIAAKQKLRQLQNLAAMVQDDDPAPQTTIANASNIGDLFLGEMEETKQQPNNVRASTNKLQKDVELNEKAREKFYEAKLQQQQQELKQLQEERRKLIEIQEKIQVLQKACPDLQLSAGLGDCTANRQTSPATSTPAMNDTAGKPSLEFDESVPVGNELWSEMRRHEILREELRQRRKQLEALMAEHQRRKELAETISTVAASVKSEGSEARRTPQQSRTENRTMATWGGSTQCALDEEDGDEDGYLSDGAGQAEEDDDDASSANDSFSVYPNNNVPENAYFVKENKDRWKNYRPLSADGNYRPLSKARQQQNISMRRQENLRWMSELSYVEEKERWQEQINQLKKQHEFSVSICQTLMQDQQTLSCLLQTLLTGPYSMMPNNVASSQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNDLVRQQEEQRYEEKSSRKDRGSGVPPPPSPVFCPFSFPPQPLNLFNVPGFTNFSSFAPDINYNPVFPSGFGDFAHNISPHSSEQQQQHPLDHNASGKTEYMAFPKPFESSSSNGAEKQRRSHRQPEEEMEKRSAWLNDSQDVKKDDQSQLKASFAVSVQNIASNHKNQSDMSRRREFDEESLESFSSMPDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKNKRKSSSQLKGRITNIDYESASASSVCEPCKSNKSRHSEEVGHAKVFSKRNREQLEKIIKYSRSTEMSSAHARRILQQSNRNACIEAPETGSDLSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHLSENNEKGKCTKSLNSAAWIASNSELTPSESLASTDDETFGKNFSTEACQDCEQNDADNGSSMSTSSNFEPFATDDLGNTVIHLDQALARMREYERMKIEAESTLDSEGCSSNFQGASAAKLEGGQSASECVPVPQSSEASAVPCPHIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSISVKQRCKRKIEATEVIQSYAKEAKKGLQVDVCSSAEDVDEDKDKDETETVKQVQDSEMYDGNRVPESIRSDASDQEEDDESESGPVAISLSKAETQALTNYGSGEDENEDEEIEFEEGPVDVQTSLQASSETTTENEQNSNQEQSKTKSSEILSSEQESVYAKGEQHVATILPYYLNVMENAPPLTVNTPESFITDSMKTEESSSSLPVNETQTLDTTCVGNKSAASSESSMAGSPDTESPVLVNEYEAGSGNVSQRSDEDDFVKVEDLPLKLAVYSEADLMKKMAMEAQTNSLSDELLGGGGAQDQELVGDAQTLKEPETFGVQSA

Target ORF information:

RefSeq Version	XM_021285867.1
Organism	Columba livia(rock pigeon)
Definition	Columba livia pericentriolar material 1 (PCM1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021285867.1

ORF Insert Sequence:

ATGGCAACAG GAGGTGGTCC CTTTGAAGAA GGCATGAATG ATCAAGACTT GCCTAGCTGG 
AGCAATGACA GCTTTGATGA CCGGCTGAAC AACACGGACT GGGGAAGTCA ACAGAAGAAA 
GCAAACAGAT CTTCAGAAAA AAACAAGAAA AAGCTTGGTG GGGAAGCTGA AACAAGGCTT 
ACTAATGATA TATCTCCAGA ATCCTCACCT GGAATAGGAC GACGAAAGAC CAGAACTCCT 
CATAGTTTTC CTCATGCTCG ATACATGACC CAGATGTCTG TTCCGGAGCA GGCTGAGCTA 
GAAAGGCTTA AACAAAGAAT AAACTTCAGT GATCTGGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGCA GGGCAACAGC TGCTAACAAC AAACGTCAAC TTAATGAAAA CAAAAAACCA 
TTCAACTTCC TGTCACTGCA GATAAACACT AACAAGAGCA AAGATCCTGC CTCATGTTCC 
CAAAAAAAGG AAAGTGGGGT GTCAGTCCAA CGTAAAGAGT TGTTTGGTGC TGCTCTGAGC 
AAGGATTTCT TGCAAAATTG TCAAGTCTCT GCTCAAGAAG ATGGAGGGGG AGAACCAGCT 
ATGGATAGTA GCCAGGCTAG ACAAAATGAG GAGGATGATG TTCGGACTAT AGATTCAGCT 
GTGGGATCTG GTTCTGTAGG TGAGAGCACA TCGCTAAACA TTGATGTGCA GTCTGAGGCT 
TCAGATACCA CGGCTAGAGA TCCTCAACAG GAGGCTAAAG AGGAGTTGGA GAACTTGAAG 
AAGCAGCATG ATTTATTGAA AAGGATGCTA AAACAGCAAG AGCAATTAAA GGCTCTTCAA 
GGAAGACAGG CAGCTCTTCT TGCTTTGCAG CATAAAGCAG AGCAAGCAAT TGCTGTCATG 
GATGATTCTG TTGTAACAGA GACTACAGGT AGTGTTTCAG GAGTAAGCCT TACATCAGAA 
CTGAATGAAG AATTGAATGA CTTAATTCAG CGCTTTCACA ACCAACTTCA TGATTCTCAG 
ACACAGTCTG TACCAGACAA TAGAAGGCAA GCAGAAAGTC TTTCACTTAC TAGAGAGGTT 
TCACAAAGCA GAAACTCTTC AATGTCTGAA CGCCTGTCAG ATGAGAAGGC GCAGCTTTTT 
AACAAGATGC GAATGTTGCA GGGTAAAAAG CAAAAAATGG ACAAACTGTT AGGAGAACTT 
CATACACTTC GTGACCAACA TCTAAATAAC TCCTCCTTTT TTCCTGCTTC AGGTTCTCCT 
CAAAGAAGTA TTGATCAAAG AAGTACAACT TCAGCTGCTT CTGGTCCTGC AGGCATAGTA 
ACTGTTGTCA ATGGCGAATC AAATAGCCTG GCATCTGCTC CCTATCCTCC TGATTCCCTG 
GTTTCTCAGA ATGAGAGTGA AGAAGATGAC AATCTAAATC CAACAGAAAA GCTTCAGAAA 
CTAAATGAAG TTCGTAAGAG GCTGAATGAG TTACGTGAGC TAGTTCACTA CTATGAGCAA 
ACATCTGATA TGATGACAGA TTCTGTGAAT GAAAACACTA AGGAAGAAGA AGAAACCGAA 
GAATCAGAAA GTGATTCTGA ACGTGAGGAT CCACAGCCTG TTACAAATAT TAGAAATCCT 
CAAGGAATCA GTAGTTGGAG TGACATAAAT AGCAACTCAA ATGTACAGTG TGGAACTAAT 
AACAGAGATG GAAGACATCT TAATACAGAC TGTGAAATAA ATAACAGATC TGCTGCTAAC 
ATAAGGACTC TAAAAATGTC GTCACCTTTA GACTGTCATA ATAGGGAGGA TGACAAAGAT 
ATTGGCCTAC CCCAATGTGA AGATGAAGAA GTGGAAGAAG ATAGAGTTAG TGAAGATTCT 
GCATCTAGTC ACAGAAGCAG CCTGGGTGAT ACTGCTGGAG ATGCTGAGTT TGAGCAGAAG 
ATCAATAGGC TTATAGCTGC AAAACAGAAA CTTAGACAAT TACAAAACCT TGCTGCTATG 
GTGCAGGATG ATGATCCAGC ACCTCAGACA ACAATTGCAA ATGCATCTAA TATTGGTGAC 
TTGTTTTTGG GTGAGATGGA AGAGACAAAG CAGCAGCCAA ACAATGTCCG AGCTAGTACC 
AACAAATTAC AAAAAGATGT AGAACTGAAT GAAAAAGCAA GAGAGAAGTT TTATGAAGCT 
AAACTCCAGC AGCAGCAACA GGAGCTTAAG CAGTTACAAG AAGAAAGAAG AAAACTGATT 
GAAATTCAAG AAAAAATTCA GGTGTTACAG AAAGCTTGTC CTGATCTTCA ATTGTCAGCT 
GGCTTGGGTG ACTGCACAGC AAATAGACAG ACTTCACCAG CAACATCAAC TCCAGCCATG 
AATGACACAG CTGGCAAGCC TTCACTTGAA TTTGATGAAT CTGTACCAGT AGGCAATGAG 
TTATGGTCTG AGATGAGAAG ACATGAGATT TTAAGAGAAG AATTGCGACA AAGAAGAAAG 
CAACTGGAAG CTCTAATGGC TGAGCATCAG AGGAGGAAAG AGCTCGCAGA AACAATATCT 
ACTGTTGCTG CGTCTGTTAA AAGTGAAGGA TCAGAAGCTC GGCGTACTCC ACAGCAGAGT 
AGGACTGAAA ATAGGACAAT GGCTACCTGG GGAGGTTCTA CGCAGTGTGC ACTAGATGAA 
GAGGATGGAG ATGAAGATGG TTATCTCTCT GATGGAGCTG GTCAGGCAGA AGAAGATGAT 
GATGATGCGT CAAGTGCGAA TGACAGCTTC TCTGTTTATC CCAATAACAA CGTACCAGAA 
AATGCATATT TTGTTAAAGA AAACAAGGAT AGGTGGAAAA ATTACCGTCC TCTTTCAGCA 
GATGGAAATT ATCGTCCATT GTCTAAGGCC AGACAACAGC AAAACATAAG TATGCGACGT 
CAGGAAAACC TTCGGTGGAT GTCTGAACTT TCATATGTGG AAGAAAAGGA ACGATGGCAA 
GAGCAGATCA ATCAGTTGAA GAAACAGCAT GAATTTAGTG TCAGCATTTG TCAAACTTTA 
ATGCAGGATC AGCAGACCCT CTCTTGTCTT CTACAGACTT TGCTTACAGG CCCTTACAGT 
ATGATGCCCA ATAATGTTGC ATCTTCACAA GTACATCTTA TTATGCATCA GCTAAACCAG 
TGTTACACTC AACTGACATG GCAGCAGAAT AACGTCCAGA GGTTGAAACA AATGTTAAAT 
GATCTTGTGC GTCAGCAAGA AGAACAACGG TATGAAGAGA AGTCATCAAG AAAGGATAGA 
GGCAGTGGTG TACCCCCACC TCCATCTCCT GTTTTCTGTC CGTTCAGCTT TCCTCCACAA 
CCTTTGAACC TGTTTAATGT ACCAGGATTT ACTAATTTTT CTTCCTTTGC TCCAGATATT 
AACTATAATC CAGTGTTTCC TTCTGGTTTT GGAGATTTTG CACACAATAT TTCTCCACAC 
AGCAGTGAGC AGCAGCAGCA ACATCCTCTA GATCATAATG CATCTGGGAA AACTGAGTAT 
ATGGCGTTCC CCAAACCTTT TGAAAGCAGT TCCTCTAATG GAGCGGAAAA ACAAAGGAGG 
AGTCACAGAC AACCTGAAGA GGAAATGGAA AAAAGATCAG CTTGGCTTAA TGATAGCCAG 
GACGTGAAAA AAGATGATCA GTCTCAGCTG AAAGCAAGTT TTGCTGTTTC AGTACAAAAC 
ATTGCTTCCA ATCATAAAAA TCAGTCTGAT ATGAGCAGAA GAAGAGAGTT TGATGAAGAG 
TCTTTGGAGA GTTTTAGTAG CATGCCTGAT CCAGTAGACC CCACTACTGT GACAAAGACA 
TTTAAATCTA GAAAAGCATC AGCACAAGCA AGTTTGGCAT CAAAAGATAA AACACCTAAA 
TCGAAGAATA AGAGGAAGAG TTCTTCTCAG CTAAAAGGCA GAATTACAAA TATTGATTAT 
GAAAGTGCCA GTGCTTCTAG TGTGTGTGAA CCCTGCAAGA GCAATAAAAG CAGACACTCT 
GAAGAGGTTG GTCATGCAAA GGTGTTCAGC AAAAGGAATC GGGAACAACT GGAAAAAATC 
ATTAAATACA GTAGATCTAC AGAAATGTCT TCAGCGCATG CTAGGAGAAT TCTGCAGCAG 
TCTAACAGAA ATGCATGCAT TGAAGCGCCA GAAACTGGTA GTGATCTTTC TATGTTTGAA 
GCTTTACGAG ACACAATTTA TTCTGAAGTG GCAACTCTTA TTTCTCAAAA TGAGTCTCGT 
CCCCACTTTC TTATTGAACT TTTCCATGAG CTTCAGCTGC TAAATACAGA TTACCTGAGG 
CAAAGAGCTC TATACGCTCT ACAGGATATA GTGACCAGAC ATTTATCTGA GAACAATGAA 
AAAGGGAAGT GCACAAAATC ACTGAATTCT GCAGCATGGA TAGCATCAAA TTCTGAACTC 
ACTCCCAGTG AAAGCCTTGC TTCTACAGAT GATGAAACTT TTGGCAAAAA CTTTTCTACA 
GAAGCATGTC AAGATTGTGA ACAAAATGAT GCAGACAATG GGAGTAGCAT GTCTACATCT 
TCAAATTTTG AACCCTTTGC CACTGATGAC CTTGGCAACA CAGTGATTCA CTTAGATCAA 
GCTTTGGCTA GGATGAGGGA GTATGAGCGT ATGAAAATTG AAGCTGAAAG TACCCTTGAC 
TCTGAGGGCT GCTCTAGTAA TTTTCAGGGT GCTTCTGCTG CTAAATTAGA AGGAGGTCAA 
AGTGCCAGTG AATGTGTTCC TGTGCCACAG TCAAGTGAAG CTTCTGCTGT TCCATGTCCT 
CATATAGACA CTCAGCAGCT TGACCGGCAG ATTAAAGCAA TTATGAAAGA GGTCATTCCT 
TTTCTGAAGG AACACATGGA TGAAGTATGC TCTTCGCAGT TACTGACATC AGTAAGACGT 
ATGGTCTTGA CCCTTACACA ACAAAATGAT GAAAGTAAAG AGTTTGTGAA GTTCTTTCAT 
AAGCAGCTTG GCAGTATACT TCAGGATTCA CTGGCAAAAT TTGCTGGTAG AAAACTGAAA 
GATTGTGGAG AGGATCTTCT AGTGGAGATC TCTGAAGTGT TATTTAATGA ATTAGCCTTT 
TTTAAACTCA TGCAAGACTT GGACAACAAC AGTATTTCTG TAAAGCAGAG ATGTAAAAGA 
AAAATAGAAG CTACTGAAGT GATACAGTCG TATGCTAAAG AGGCAAAAAA AGGTCTCCAG 
GTGGATGTTT GTTCATCTGC TGAAGATGTC GATGAGGACA AAGACAAGGA TGAGACTGAA 
ACTGTTAAAC AAGTACAGGA CTCAGAAATG TATGATGGGA ATAGAGTTCC TGAAAGTATT 
AGGTCTGATG CATCTGATCA GGAGGAAGAT GATGAAAGTG AAAGCGGTCC AGTGGCAATA 
AGTTTATCAA AAGCAGAAAC CCAAGCTCTG ACTAACTATG GTAGTGGAGA AGATGAGAAT 
GAAGATGAAG AAATAGAATT TGAGGAAGGA CCTGTTGATG TGCAGACGTC ATTACAAGCC 
AGCAGTGAAA CAACAACTGA AAATGAACAG AATTCAAACC AAGAACAAAG TAAGACAAAA 
AGCAGTGAGA TTCTGTCATC AGAACAAGAA TCTGTTTATG CTAAAGGTGA ACAACATGTG 
GCTACAATTT TGCCTTATTA CCTCAATGTA ATGGAGAATG CACCGCCTTT AACGGTCAAT 
ACCCCAGAAT CTTTTATAAC AGACAGTATG AAAACAGAAG AATCAAGTTC ATCTTTACCA 
GTAAATGAAA CTCAAACACT GGATACAACG TGTGTAGGAA ACAAATCTGC TGCAAGTTCT 
GAAAGCTCCA TGGCTGGCAG CCCTGATACA GAGTCACCTG TGTTAGTGAA TGAATATGAA 
GCTGGTTCTG GAAATGTAAG TCAAAGATCT GATGAAGATG ACTTTGTGAA AGTTGAAGAC 
TTGCCCCTTA AACTTGCAGT GTATTCAGAG GCAGATTTAA TGAAGAAAAT GGCAATGGAG 
GCCCAAACGA ACAGTTTGTC TGATGAATTA CTGGGTGGAG GTGGAGCTCA AGATCAAGAA 
TTAGTAGGAG ATGCCCAAAC TTTGAAAGAA CCTGAAACTT TTGGAGTTCA AAGTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_021285870.1 ) cDNA ORF clone, Columba livia(rock pigeon) -> XP_021141545.1 Columba livia pericentriolar material 1 (PCM1), transcript variant X17, mRNA.

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CloneID	OCo293173
Clone ID Related Accession (Same CDS sequence)	XM_021285870.1
Accession Version	XM_021285870.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-23
Organism	Columba livia(rock pigeon)
Product	pericentriolar material 1 protein isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004973253.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Columba livia Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACAG GAGGTGGTCC CTTTGAAGAA GGCATGAATG ATCAAGACTT GCCTAGCTGG 
AGCAATGACA GCTTTGATGA CCGGCTGAAC AACACGGACT GGGGAAGTCA ACAGAAGAAA 
GCAAACAGAT CTTCAGAAAA AAACAAGAAA AAGCTTGGTG GGGAAGCTGA AACAAGGCTT 
ACTAATGATA TATCTCCAGA ATCCTCACCT GGAATAGGAC GACGAAAGAC CAGAACTCCT 
CATAGTTTTC CTCATGCTCG ATACATGACC CAGATGTCTG TTCCGGAGCA GGCTGAGCTA 
GAAAGGCTTA AACAAAGAAT AAACTTCAGT GATCTGGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGCA GGGCAACAGC TGCTAACAAC AAACGTCAAC TTAATGAAAA CAAAAAACCA 
TTCAACTTCC TGTCACTGCA GATAAACACT AACAAGAGCA AAGATCCTGC CTCATGTTCC 
CAAAAAAAGG AAAGTGGGGT GTCAGTCCAA CGTAAAGAGT TGTTTGGTGC TGCTCTGAGC 
AAGGATTTCT TGCAAAATTG TCAAGTCTCT GCTCAAGAAG ATGGAGGGGG AGAACCAGCT 
ATGGATAGTA GCCAGATTGT GAGCAGACTA GTTCAAATTC GCGACTATAT TGCTAAGGCC 
AGCTCCATGC GGGATGATCT TGTAGAGAAA AATGAAAGAT CGGCCAATGT TGAGCGTTTA 
TCACACCTTA TAGATCACCT TAAAGAGCAG GAGAAATCCT ATCTGAAATT TTTGCAAAAG 
ATGCTTGCTA GAGATCCTCA ACAGGAGGCT AAAGAGGAGT TGGAGAACTT GAAGAAGCAG 
CATGATTTAT TGAAAAGGAT GCTAAAACAG CAAGAGCAAT TAAAGGCTCT TCAAGGAAGA 
CAGGCAGCTC TTCTTGCTTT GCAGCATAAA GCAGAGCAAG CAATTGCTGT CATGGATGAT 
TCTGTTGTAA CAGAGACTAC AGGTAGTGTT TCAGGAGTAA GCCTTACATC AGAACTGAAT 
GAAGAATTGA ATGACTTAAT TCAGCGCTTT CACAACCAAC TTCATGATTC TCAGACACAG 
TCTGTACCAG ACAATAGAAG GCAAGCAGAA AGTCTTTCAC TTACTAGAGA GGTTTCACAA 
AGCAGAAACT CTTCAATGTC TGAACGCCTG TCAGATGAGA AGGCGCAGCT TTTTAACAAG 
ATGCGAATGT TGCAGGGTAA AAAGCAAAAA ATGGACAAAC TGTTAGGAGA ACTTCATACA 
CTTCGTGACC AACATCTAAA TAACTCCTCC TTTTTTCCTG CTTCAGGTTC TCCTCAAAGA 
AGTATTGATC AAAGAAGTAC AACTTCAGCT GCTTCTGGTC CTGCAGGCAT AGTAACTGTT 
GTCAATGGCG AATCAAATAG CCTGGCATCT GCTCCCTATC CTCCTGATTC CCTGGTTTCT 
CAGAATGAGA GTGAAGAAGA TGACAATCTA AATCCAACAG AAAAGCTTCA GAAACTAAAT 
GAAGTTCGTA AGAGGCTGAA TGAGTTACGT GAGCTAGTTC ACTACTATGA GCAAACATCT 
GATATGATGA CAGATTCTGT GAATGAAAAC ACTAAGGAAG AAGAAGAAAC CGAAGAATCA 
GAAAGTGATT CTGAACGTGA GGATCCACAG CCTGTTACAA ATATTAGAAA TCCTCAAGGA 
ATCAGTAGTT GGAGTGACAT AAATAGCAAC TCAAATGTAC AGTGTGGAAC TAATAACAGA 
GATGGAAGAC ATCTTAATAC AGACTGTGAA ATAAATAACA GATCTGCTGC TAACATAAGG 
ACTCTAAAAA TGTCGTCACC TTTAGACTGT CATAATAGGG AGGATGACAA AGATATTGGC 
CTACCCCAAT GTGAAGATGA AGAAGTGGAA GAAGATAGAG TTAGTGAAGA TTCTGCATCT 
AGTCACAGAA GCAGCCTGGG TGATACTGCT GGAGATGCTG AGTTTGAGCA GAAGATCAAT 
AGGCTTATAG CTGCAAAACA GAAACTTAGA CAATTACAAA ACCTTGCTGC TATGGTGCAG 
GATGATGATC CAGCACCTCA GACAACAATT GCAAATGCAT CTAATATTGG TGACTTGTTT 
TTGGGTGAGA TGGAAGAGAC AAAGCAGCAG CCAAACAATG TCCGAGCTAG TACCAACAAA 
TTACAAAAAG ATGTAGAACT GAATGAAAAA GCAAGAGAGA AGTTTTATGA AGCTAAACTC 
CAGCAGCAGC AACAGGAGCT TAAGCAGTTA CAAGAAGAAA GAAGAAAACT GATTGAAATT 
CAAGAAAAAA TTCAGGTGTT ACAGAAAGCT TGTCCTGATC TTCAATTGTC AGCTGGCTTG 
GGTGACTGCA CAGCAAATAG ACAGACTTCA CCAGCAACAT CAACTCCAGC CATGAATGAC 
ACAGCTGGCA AGCCTTCACT TGAATTTGAT GAATCTGTAC CAGTAGGCAA TGAGTTATGG 
TCTGAGATGA GAAGACATGA GATTTTAAGA GAAGAATTGC GACAAAGAAG AAAGCAACTG 
GAAGCTCTAA TGGCTGAGCA TCAGAGGAGG AAAGAGCTCG CAGAAACAAT ATCTACTGTT 
GCTGCGTCTG TTAAAAGTGA AGGATCAGAA GCTCGGCGTA CTCCACAGCA GAGTAGGACT 
GAAAAGACAA TGGCTACCTG GGGAGGTTCT ACGCAGTGTG CACTAGATGA AGAGGATGGA 
GATGAAGATG GTTATCTCTC TGATGGAGCT GGTCAGGCAG AAGAAGATGA TGATGATGCG 
TCAAGTGCGA ATGACAGCTT CTCTGTTTAT CCCAATAACA ACGTACCAGA AAATGCATAT 
TTTGTTAAAG AAAACAAGGA TAGGTGGAAA AATTACCGTC CTCTTTCAGC AGATGGAAAT 
TATCGTCCAT TGTCTAAGGC CAGACAACAG CAAAACATAA GTATGCGACG TCAGGAAAAC 
CTTCGGTGGA TGTCTGAACT TTCATATGTG GAAGAAAAGG AACGATGGCA AGAGCAGATC 
AATCAGTTGA AGAAACAGCA TGAATTTAGT GTCAGCATTT GTCAAACTTT AATGCAGGAT 
CAGCAGACCC TCTCTTGTCT TCTACAGACT TTGCTTACAG GCCCTTACAG TATGATGCCC 
AATAATGTTG CATCTTCACA AGTACATCTT ATTATGCATC AGCTAAACCA GTGTTACACT 
CAACTGACAT GGCAGCAGAA TAACGTCCAG AGGTTGAAAC AAATGTTAAA TGATCTTGTG 
CGTCAGCAAG AAGAACAACG GTATGAAGAG AAGTCATCAA GAAAGGATAG AGGCAGTGGT 
GTACCCCCAC CTCCATCTCC TGTTTTCTGT CCGTTCAGCT TTCCTCCACA ACCTTTGAAC 
CTGTTTAATG TACCAGGATT TACTAATTTT TCTTCCTTTG CTCCAGATAT TAACTATAAT 
CCAGTGTTTC CTTCTGGTTT TGGAGATTTT GCACACAATA TTTCTCCACA CAGCAGTGAG 
CAGCAGCAGC AACATCCTCT AGATCATAAT GCATCTGGGA AAACTGAGTA TATGGCGTTC 
CCCAAACCTT TTGAAAGCAG TTCCTCTAAT GGAGCGGAAA AACAAAGGAG GAGTCACAGA 
CAACCTGAAG AGGAAATGGA AAAAAGATCA GCTTGGCTTA ATGATAGCCA GGACGTGAAA 
AAAGATGATC AGTCTCAGCT GAAAGCAAGT TTTGCTGTTT CAGTACAAAA CATTGCTTCC 
AATCATAAAA ATCAGTCTGA TATGAGCAGA AGAAGAGAGT TTGATGAAGA GTCTTTGGAG 
AGTTTTAGTA GCATGCCTGA TCCAGTAGAC CCCACTACTG TGACAAAGAC ATTTAAATCT 
AGAAAAGCAT CAGCACAAGC AAGTTTGGCA TCAAAAGATA AAACACCTAA ATCGAAGAAT 
AAGAGGAAGA GTTCTTCTCA GCTAAAAGGC AGAATTACAA ATATTGATTA TGAAAGTGCC 
AGTGCTTCTA GTGTGTGTGA ACCCTGCAAG AGCAATAAAA GCAGACACTC TGAAGAGGTT 
GGTCATGCAA AGGTGTTCAG CAAAAGGAAT CGGGAACAAC TGGAAAAAAT CATTAAATAC 
AGTAGATCTA CAGAAATGTC TTCAGAAACT GGTAGTGATC TTTCTATGTT TGAAGCTTTA 
CGAGACACAA TTTATTCTGA AGTGGCAACT CTTATTTCTC AAAATGAGTC TCGTCCCCAC 
TTTCTTATTG AACTTTTCCA TGAGCTTCAG CTGCTAAATA CAGATTACCT GAGGCAAAGA 
GCTCTATACG CTCTACAGGA TATAGTGACC AGACATTTAT CTGAGAACAA TGAAAAAGGG 
AAGTGCACAA AATCACTGAA TTCTGCAGCA TGGATAGCAT CAAATTCTGA ACTCACTCCC 
AGTGAAAGCC TTGCTTCTAC AGATGATGAA ACTTTTGGCA AAAACTTTTC TACAGAAGCA 
TGTCAAGATT GTGAACAAAA TGATGCAGAC AATGGGAGTA GCATGTCTAC ATCTTCAAAT 
TTTGAACCCT TTGCCACTGA TGACCTTGGC AACACAGTGA TTCACTTAGA TCAAGCTTTG 
GCTAGGATGA GGGAGTATGA GCGTATGAAA ATTGAAGCTG AAAGTACCCT TGACTCTGAG 
GGCTGCTCTA GTAATTTTCA GGGTGCTTCT GCTGCTAAAT TAGAAGGAGG TCAAAGTGCC 
AGTGAATGTG TTCCTGTGCC ACAGTCAAGT GAAGCTTCTG CTGTTCCATG TCCTCATATA 
GACACTCAGC AGCTTGACCG GCAGATTAAA GCAATTATGA AAGAGGTCAT TCCTTTTCTG 
AAGGAACACA TGGATGAAGT ATGCTCTTCG CAGTTACTGA CATCAGTAAG ACGTATGGTC 
TTGACCCTTA CACAACAAAA TGATGAAAGT AAAGAGTTTG TGAAGTTCTT TCATAAGCAG 
CTTGGCAGTA TACTTCAGGA TTCACTGGCA AAATTTGCTG GTAGAAAACT GAAAGATTGT 
GGAGAGGATC TTCTAGTGGA GATCTCTGAA GTGTTATTTA ATGAATTAGC CTTTTTTAAA 
CTCATGCAAG ACTTGGACAA CAACAGTATT TCTGTAAAGC AGAGATGTAA AAGAAAAATA 
GAAGCTACTG AAGTGATACA GTCGTATGCT AAAGAGGCAA AAAAAGGTCT CCAGGTGGAT 
GTTTGTTCAT CTGCTGAAGA TGTCGATGAG GACAAAGACA AGGATGAGAC TGAAACTGTT 
AAACAAGTAC AGGACTCAGA AATGTATGAT GGGAATAGAG TTCCTGAAAG TATTAGGTCT 
GATGCATCTG ATCAGGAGGA AGATGATGAA AGTGAAAGCG GTCCAGTGGC AATAAGTTTA 
TCAAAAGCAG AAACCCAAGC TCTGACTAAC TATGGTAGTG GAGAAGATGA GAATGAAGAT 
GAAGAAATAG AATTTGAGGA AGGACCTGTT GATGTGCAGA CGTCATTACA AGCCAGCAGT 
GAAACAACAA CTGAAAATGA ACAGAATTCA AACCAAGAAC AAAGTAAGAC AAAAAGCAGT 
GAGATTCTGT CATCAGAACA AGAATCTGTT TATGCTAAAG GTGAACAACA TGTGGCTACA 
ATTTTGCCTT ATTACCTCAA TGTAATGGAG AATGCACCGC CTTTAACGGT CAATACCCCA 
GAATCTTTTA TAACAGACAG TATGAAAACA GAAGAATCAA GTTCATCTTT ACCAGTAAAT 
GAAACTCAAA CACTGGATAC AACGTGTGTA GGAAACAAAT CTGCTGCAAG TTCTGAAAGC 
TCCATGGCTG GCAGCCCTGA TACAGAGTCA CCTGTGTTAG TGAATGAATA TGAAGCTGGT 
TCTGGAAATG TAAGTCAAAG ATCTGATGAA GATGACTTTG TGAAAGTTGA AGACTTGCCC 
CTTAAACTTG CAGTGTATTC AGAGGCAGAT TTAATGAAGA AAATGGCAAT GGAGGCCCAA 
ACGAACAGTT TGTCTGATGA ATTACTGGGT GGAGGTGGAG CTCAAGATCA AGAATTAGTA 
GGAGATGCCC AAACTTTGAA AGAACCTGAA ACTTTTGGAG TTCAAAGTGC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021141545.1
CDS	140..6193
Translation	MATGGGPFEEGMNDQDLPSWSNDSFDDRLNNTDWGSQQKKANRSSEKNKKKLGGEAETRLTNDISPESSPGIGRRKTRTPHSFPHARYMTQMSVPEQAELERLKQRINFSDLDQRSIGSDSQGRATAANNKRQLNENKKPFNFLSLQINTNKSKDPASCSQKKESGVSVQRKELFGAALSKDFLQNCQVSAQEDGGGEPAMDSSQIVSRLVQIRDYIAKASSMRDDLVEKNERSANVERLSHLIDHLKEQEKSYLKFLQKMLARDPQQEAKEELENLKKQHDLLKRMLKQQEQLKALQGRQAALLALQHKAEQAIAVMDDSVVTETTGSVSGVSLTSELNEELNDLIQRFHNQLHDSQTQSVPDNRRQAESLSLTREVSQSRNSSMSERLSDEKAQLFNKMRMLQGKKQKMDKLLGELHTLRDQHLNNSSFFPASGSPQRSIDQRSTTSAASGPAGIVTVVNGESNSLASAPYPPDSLVSQNESEEDDNLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDSVNENTKEEEETEESESDSEREDPQPVTNIRNPQGISSWSDINSNSNVQCGTNNRDGRHLNTDCEINNRSAANIRTLKMSSPLDCHNREDDKDIGLPQCEDEEVEEDRVSEDSASSHRSSLGDTAGDAEFEQKINRLIAAKQKLRQLQNLAAMVQDDDPAPQTTIANASNIGDLFLGEMEETKQQPNNVRASTNKLQKDVELNEKAREKFYEAKLQQQQQELKQLQEERRKLIEIQEKIQVLQKACPDLQLSAGLGDCTANRQTSPATSTPAMNDTAGKPSLEFDESVPVGNELWSEMRRHEILREELRQRRKQLEALMAEHQRRKELAETISTVAASVKSEGSEARRTPQQSRTEKTMATWGGSTQCALDEEDGDEDGYLSDGAGQAEEDDDDASSANDSFSVYPNNNVPENAYFVKENKDRWKNYRPLSADGNYRPLSKARQQQNISMRRQENLRWMSELSYVEEKERWQEQINQLKKQHEFSVSICQTLMQDQQTLSCLLQTLLTGPYSMMPNNVASSQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNDLVRQQEEQRYEEKSSRKDRGSGVPPPPSPVFCPFSFPPQPLNLFNVPGFTNFSSFAPDINYNPVFPSGFGDFAHNISPHSSEQQQQHPLDHNASGKTEYMAFPKPFESSSSNGAEKQRRSHRQPEEEMEKRSAWLNDSQDVKKDDQSQLKASFAVSVQNIASNHKNQSDMSRRREFDEESLESFSSMPDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKNKRKSSSQLKGRITNIDYESASASSVCEPCKSNKSRHSEEVGHAKVFSKRNREQLEKIIKYSRSTEMSSETGSDLSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHLSENNEKGKCTKSLNSAAWIASNSELTPSESLASTDDETFGKNFSTEACQDCEQNDADNGSSMSTSSNFEPFATDDLGNTVIHLDQALARMREYERMKIEAESTLDSEGCSSNFQGASAAKLEGGQSASECVPVPQSSEASAVPCPHIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSISVKQRCKRKIEATEVIQSYAKEAKKGLQVDVCSSAEDVDEDKDKDETETVKQVQDSEMYDGNRVPESIRSDASDQEEDDESESGPVAISLSKAETQALTNYGSGEDENEDEEIEFEEGPVDVQTSLQASSETTTENEQNSNQEQSKTKSSEILSSEQESVYAKGEQHVATILPYYLNVMENAPPLTVNTPESFITDSMKTEESSSSLPVNETQTLDTTCVGNKSAASSESSMAGSPDTESPVLVNEYEAGSGNVSQRSDEDDFVKVEDLPLKLAVYSEADLMKKMAMEAQTNSLSDELLGGGGAQDQELVGDAQTLKEPETFGVQSA

Target ORF information:

RefSeq Version	XM_021285870.1
Organism	Columba livia(rock pigeon)
Definition	Columba livia pericentriolar material 1 (PCM1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021285870.1

ORF Insert Sequence:

ATGGCAACAG GAGGTGGTCC CTTTGAAGAA GGCATGAATG ATCAAGACTT GCCTAGCTGG 
AGCAATGACA GCTTTGATGA CCGGCTGAAC AACACGGACT GGGGAAGTCA ACAGAAGAAA 
GCAAACAGAT CTTCAGAAAA AAACAAGAAA AAGCTTGGTG GGGAAGCTGA AACAAGGCTT 
ACTAATGATA TATCTCCAGA ATCCTCACCT GGAATAGGAC GACGAAAGAC CAGAACTCCT 
CATAGTTTTC CTCATGCTCG ATACATGACC CAGATGTCTG TTCCGGAGCA GGCTGAGCTA 
GAAAGGCTTA AACAAAGAAT AAACTTCAGT GATCTGGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGCA GGGCAACAGC TGCTAACAAC AAACGTCAAC TTAATGAAAA CAAAAAACCA 
TTCAACTTCC TGTCACTGCA GATAAACACT AACAAGAGCA AAGATCCTGC CTCATGTTCC 
CAAAAAAAGG AAAGTGGGGT GTCAGTCCAA CGTAAAGAGT TGTTTGGTGC TGCTCTGAGC 
AAGGATTTCT TGCAAAATTG TCAAGTCTCT GCTCAAGAAG ATGGAGGGGG AGAACCAGCT 
ATGGATAGTA GCCAGATTGT GAGCAGACTA GTTCAAATTC GCGACTATAT TGCTAAGGCC 
AGCTCCATGC GGGATGATCT TGTAGAGAAA AATGAAAGAT CGGCCAATGT TGAGCGTTTA 
TCACACCTTA TAGATCACCT TAAAGAGCAG GAGAAATCCT ATCTGAAATT TTTGCAAAAG 
ATGCTTGCTA GAGATCCTCA ACAGGAGGCT AAAGAGGAGT TGGAGAACTT GAAGAAGCAG 
CATGATTTAT TGAAAAGGAT GCTAAAACAG CAAGAGCAAT TAAAGGCTCT TCAAGGAAGA 
CAGGCAGCTC TTCTTGCTTT GCAGCATAAA GCAGAGCAAG CAATTGCTGT CATGGATGAT 
TCTGTTGTAA CAGAGACTAC AGGTAGTGTT TCAGGAGTAA GCCTTACATC AGAACTGAAT 
GAAGAATTGA ATGACTTAAT TCAGCGCTTT CACAACCAAC TTCATGATTC TCAGACACAG 
TCTGTACCAG ACAATAGAAG GCAAGCAGAA AGTCTTTCAC TTACTAGAGA GGTTTCACAA 
AGCAGAAACT CTTCAATGTC TGAACGCCTG TCAGATGAGA AGGCGCAGCT TTTTAACAAG 
ATGCGAATGT TGCAGGGTAA AAAGCAAAAA ATGGACAAAC TGTTAGGAGA ACTTCATACA 
CTTCGTGACC AACATCTAAA TAACTCCTCC TTTTTTCCTG CTTCAGGTTC TCCTCAAAGA 
AGTATTGATC AAAGAAGTAC AACTTCAGCT GCTTCTGGTC CTGCAGGCAT AGTAACTGTT 
GTCAATGGCG AATCAAATAG CCTGGCATCT GCTCCCTATC CTCCTGATTC CCTGGTTTCT 
CAGAATGAGA GTGAAGAAGA TGACAATCTA AATCCAACAG AAAAGCTTCA GAAACTAAAT 
GAAGTTCGTA AGAGGCTGAA TGAGTTACGT GAGCTAGTTC ACTACTATGA GCAAACATCT 
GATATGATGA CAGATTCTGT GAATGAAAAC ACTAAGGAAG AAGAAGAAAC CGAAGAATCA 
GAAAGTGATT CTGAACGTGA GGATCCACAG CCTGTTACAA ATATTAGAAA TCCTCAAGGA 
ATCAGTAGTT GGAGTGACAT AAATAGCAAC TCAAATGTAC AGTGTGGAAC TAATAACAGA 
GATGGAAGAC ATCTTAATAC AGACTGTGAA ATAAATAACA GATCTGCTGC TAACATAAGG 
ACTCTAAAAA TGTCGTCACC TTTAGACTGT CATAATAGGG AGGATGACAA AGATATTGGC 
CTACCCCAAT GTGAAGATGA AGAAGTGGAA GAAGATAGAG TTAGTGAAGA TTCTGCATCT 
AGTCACAGAA GCAGCCTGGG TGATACTGCT GGAGATGCTG AGTTTGAGCA GAAGATCAAT 
AGGCTTATAG CTGCAAAACA GAAACTTAGA CAATTACAAA ACCTTGCTGC TATGGTGCAG 
GATGATGATC CAGCACCTCA GACAACAATT GCAAATGCAT CTAATATTGG TGACTTGTTT 
TTGGGTGAGA TGGAAGAGAC AAAGCAGCAG CCAAACAATG TCCGAGCTAG TACCAACAAA 
TTACAAAAAG ATGTAGAACT GAATGAAAAA GCAAGAGAGA AGTTTTATGA AGCTAAACTC 
CAGCAGCAGC AACAGGAGCT TAAGCAGTTA CAAGAAGAAA GAAGAAAACT GATTGAAATT 
CAAGAAAAAA TTCAGGTGTT ACAGAAAGCT TGTCCTGATC TTCAATTGTC AGCTGGCTTG 
GGTGACTGCA CAGCAAATAG ACAGACTTCA CCAGCAACAT CAACTCCAGC CATGAATGAC 
ACAGCTGGCA AGCCTTCACT TGAATTTGAT GAATCTGTAC CAGTAGGCAA TGAGTTATGG 
TCTGAGATGA GAAGACATGA GATTTTAAGA GAAGAATTGC GACAAAGAAG AAAGCAACTG 
GAAGCTCTAA TGGCTGAGCA TCAGAGGAGG AAAGAGCTCG CAGAAACAAT ATCTACTGTT 
GCTGCGTCTG TTAAAAGTGA AGGATCAGAA GCTCGGCGTA CTCCACAGCA GAGTAGGACT 
GAAAAGACAA TGGCTACCTG GGGAGGTTCT ACGCAGTGTG CACTAGATGA AGAGGATGGA 
GATGAAGATG GTTATCTCTC TGATGGAGCT GGTCAGGCAG AAGAAGATGA TGATGATGCG 
TCAAGTGCGA ATGACAGCTT CTCTGTTTAT CCCAATAACA ACGTACCAGA AAATGCATAT 
TTTGTTAAAG AAAACAAGGA TAGGTGGAAA AATTACCGTC CTCTTTCAGC AGATGGAAAT 
TATCGTCCAT TGTCTAAGGC CAGACAACAG CAAAACATAA GTATGCGACG TCAGGAAAAC 
CTTCGGTGGA TGTCTGAACT TTCATATGTG GAAGAAAAGG AACGATGGCA AGAGCAGATC 
AATCAGTTGA AGAAACAGCA TGAATTTAGT GTCAGCATTT GTCAAACTTT AATGCAGGAT 
CAGCAGACCC TCTCTTGTCT TCTACAGACT TTGCTTACAG GCCCTTACAG TATGATGCCC 
AATAATGTTG CATCTTCACA AGTACATCTT ATTATGCATC AGCTAAACCA GTGTTACACT 
CAACTGACAT GGCAGCAGAA TAACGTCCAG AGGTTGAAAC AAATGTTAAA TGATCTTGTG 
CGTCAGCAAG AAGAACAACG GTATGAAGAG AAGTCATCAA GAAAGGATAG AGGCAGTGGT 
GTACCCCCAC CTCCATCTCC TGTTTTCTGT CCGTTCAGCT TTCCTCCACA ACCTTTGAAC 
CTGTTTAATG TACCAGGATT TACTAATTTT TCTTCCTTTG CTCCAGATAT TAACTATAAT 
CCAGTGTTTC CTTCTGGTTT TGGAGATTTT GCACACAATA TTTCTCCACA CAGCAGTGAG 
CAGCAGCAGC AACATCCTCT AGATCATAAT GCATCTGGGA AAACTGAGTA TATGGCGTTC 
CCCAAACCTT TTGAAAGCAG TTCCTCTAAT GGAGCGGAAA AACAAAGGAG GAGTCACAGA 
CAACCTGAAG AGGAAATGGA AAAAAGATCA GCTTGGCTTA ATGATAGCCA GGACGTGAAA 
AAAGATGATC AGTCTCAGCT GAAAGCAAGT TTTGCTGTTT CAGTACAAAA CATTGCTTCC 
AATCATAAAA ATCAGTCTGA TATGAGCAGA AGAAGAGAGT TTGATGAAGA GTCTTTGGAG 
AGTTTTAGTA GCATGCCTGA TCCAGTAGAC CCCACTACTG TGACAAAGAC ATTTAAATCT 
AGAAAAGCAT CAGCACAAGC AAGTTTGGCA TCAAAAGATA AAACACCTAA ATCGAAGAAT 
AAGAGGAAGA GTTCTTCTCA GCTAAAAGGC AGAATTACAA ATATTGATTA TGAAAGTGCC 
AGTGCTTCTA GTGTGTGTGA ACCCTGCAAG AGCAATAAAA GCAGACACTC TGAAGAGGTT 
GGTCATGCAA AGGTGTTCAG CAAAAGGAAT CGGGAACAAC TGGAAAAAAT CATTAAATAC 
AGTAGATCTA CAGAAATGTC TTCAGAAACT GGTAGTGATC TTTCTATGTT TGAAGCTTTA 
CGAGACACAA TTTATTCTGA AGTGGCAACT CTTATTTCTC AAAATGAGTC TCGTCCCCAC 
TTTCTTATTG AACTTTTCCA TGAGCTTCAG CTGCTAAATA CAGATTACCT GAGGCAAAGA 
GCTCTATACG CTCTACAGGA TATAGTGACC AGACATTTAT CTGAGAACAA TGAAAAAGGG 
AAGTGCACAA AATCACTGAA TTCTGCAGCA TGGATAGCAT CAAATTCTGA ACTCACTCCC 
AGTGAAAGCC TTGCTTCTAC AGATGATGAA ACTTTTGGCA AAAACTTTTC TACAGAAGCA 
TGTCAAGATT GTGAACAAAA TGATGCAGAC AATGGGAGTA GCATGTCTAC ATCTTCAAAT 
TTTGAACCCT TTGCCACTGA TGACCTTGGC AACACAGTGA TTCACTTAGA TCAAGCTTTG 
GCTAGGATGA GGGAGTATGA GCGTATGAAA ATTGAAGCTG AAAGTACCCT TGACTCTGAG 
GGCTGCTCTA GTAATTTTCA GGGTGCTTCT GCTGCTAAAT TAGAAGGAGG TCAAAGTGCC 
AGTGAATGTG TTCCTGTGCC ACAGTCAAGT GAAGCTTCTG CTGTTCCATG TCCTCATATA 
GACACTCAGC AGCTTGACCG GCAGATTAAA GCAATTATGA AAGAGGTCAT TCCTTTTCTG 
AAGGAACACA TGGATGAAGT ATGCTCTTCG CAGTTACTGA CATCAGTAAG ACGTATGGTC 
TTGACCCTTA CACAACAAAA TGATGAAAGT AAAGAGTTTG TGAAGTTCTT TCATAAGCAG 
CTTGGCAGTA TACTTCAGGA TTCACTGGCA AAATTTGCTG GTAGAAAACT GAAAGATTGT 
GGAGAGGATC TTCTAGTGGA GATCTCTGAA GTGTTATTTA ATGAATTAGC CTTTTTTAAA 
CTCATGCAAG ACTTGGACAA CAACAGTATT TCTGTAAAGC AGAGATGTAA AAGAAAAATA 
GAAGCTACTG AAGTGATACA GTCGTATGCT AAAGAGGCAA AAAAAGGTCT CCAGGTGGAT 
GTTTGTTCAT CTGCTGAAGA TGTCGATGAG GACAAAGACA AGGATGAGAC TGAAACTGTT 
AAACAAGTAC AGGACTCAGA AATGTATGAT GGGAATAGAG TTCCTGAAAG TATTAGGTCT 
GATGCATCTG ATCAGGAGGA AGATGATGAA AGTGAAAGCG GTCCAGTGGC AATAAGTTTA 
TCAAAAGCAG AAACCCAAGC TCTGACTAAC TATGGTAGTG GAGAAGATGA GAATGAAGAT 
GAAGAAATAG AATTTGAGGA AGGACCTGTT GATGTGCAGA CGTCATTACA AGCCAGCAGT 
GAAACAACAA CTGAAAATGA ACAGAATTCA AACCAAGAAC AAAGTAAGAC AAAAAGCAGT 
GAGATTCTGT CATCAGAACA AGAATCTGTT TATGCTAAAG GTGAACAACA TGTGGCTACA 
ATTTTGCCTT ATTACCTCAA TGTAATGGAG AATGCACCGC CTTTAACGGT CAATACCCCA 
GAATCTTTTA TAACAGACAG TATGAAAACA GAAGAATCAA GTTCATCTTT ACCAGTAAAT 
GAAACTCAAA CACTGGATAC AACGTGTGTA GGAAACAAAT CTGCTGCAAG TTCTGAAAGC 
TCCATGGCTG GCAGCCCTGA TACAGAGTCA CCTGTGTTAG TGAATGAATA TGAAGCTGGT 
TCTGGAAATG TAAGTCAAAG ATCTGATGAA GATGACTTTG TGAAAGTTGA AGACTTGCCC 
CTTAAACTTG CAGTGTATTC AGAGGCAGAT TTAATGAAGA AAATGGCAAT GGAGGCCCAA 
ACGAACAGTT TGTCTGATGA ATTACTGGGT GGAGGTGGAG CTCAAGATCA AGAATTAGTA 
GGAGATGCCC AAACTTTGAA AGAACCTGAA ACTTTTGGAG TTCAAAGTGC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCM1 ( XM_021285871.1 ) cDNA ORF clone, Columba livia(rock pigeon) -> XP_021141546.1 Columba livia pericentriolar material 1 (PCM1), transcript variant X18, mRNA.

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CloneID	OCo293174
Clone ID Related Accession (Same CDS sequence)	XM_021285871.1
Accession Version	XM_021285871.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-23
Organism	Columba livia(rock pigeon)
Product	pericentriolar material 1 protein isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004973253.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Columba livia Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACAG GAGGTGGTCC CTTTGAAGAA GGCATGAATG ATCAAGACTT GCCTAGCTGG 
AGCAATGACA GCTTTGATGA CCGGCTGAAC AACACGGACT GGGGAAGTCA ACAGAAGAAA 
GCAAACAGAT CTTCAGAAAA AAACAAGAAA AAGCTTGGTG GGGAAGCTGA AACAAGGCTT 
ACTAATGATA TATCTCCAGA ATCCTCACCT GGAATAGGAC GACGAAAGAC CAGAACTCCT 
CATAGTTTTC CTCATGCTCG ATACATGACC CAGATGTCTG TTCCGGAGCA GGCTGAGCTA 
GAAAGGCTTA AACAAAGAAT AAACTTCAGT GATCTGGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGCA GGGCAACAGC TGCTAACAAC AAACGTCAAC TTAATGAAAA CAAAAAACCA 
TTCAACTTCC TGTCACTGCA GATAAACACT AACAAGAGCA AAGATCCTGC CTCATGTTCC 
CAAAAAAAGG AAAGTGGGGT GTCAGTCCAA CGTAAAGAGT TGTTTGGTGC TGCTCTGAGC 
AAGGATTTCT TGCAAAATTG TCAAGTCTCT GCTCAAGAAG ATGGAGGGGG AGAACCAGCT 
ATGGATAGTA GCCAGATTGT GAGCAGACTA GTTCAAATTC GCGACTATAT TGCTAAGGCC 
AGCTCCATGC GGGATGATCT TGTAGAGAAA AATGAAAGAT CGGCCAATGT TGAGCGTTTA 
TCACACCTTA TAGATCACCT TAAAGAGCAG GAGAAATCCT ATCTGAAATT TTTGCAAAAG 
ATGCTTGCTA GACAAAATGA GGAGGATGAT GTTCGGACTA TAGATTCAGC TGTGGGATCT 
GGTTCTGTAG GTGAGAGCAC ATCGCTAAAC ATTGATGTGC AGTCTGAGGC TTCAGATACC 
ACGGCTAGAG ATCCTCAACA GGAGGCTAAA GAGGAGTTGG AGAACTTGAA GAAGCAGCAT 
GATTTATTGA AAAGGATGCT AAAACAGCAA GAGCAATTAA AGGCTCTTCA AGGAAGACAG 
GCAGCTCTTC TTGCTTTGCA GCATAAAGCA GAGCAAGCAA TTGCTGTCAT GGATGATTCT 
GTTGTAACAG AGACTACAGG TAGTGTTTCA GGAGTAAGCC TTACATCAGA ACTGAATGAA 
GAATTGAATG ACTTAATTCA GCGCTTTCAC AACCAACTTC ATGATTCTCA GACACAGTCT 
GTACCAGACA ATAGAAGGCA AGCAGAAAGT CTTTCACTTA CTAGAGAGGT TTCACAAAGC 
AGAAACTCTT CAATGTCTGA ACGCCTGTCA GATGAGAAGG CGCAGCTTTT TAACAAGATG 
CGAATGTTGC AGGGTAAAAA GCAAAAAATG GACAAACTGT TAGGAGAACT TCATACACTT 
CGTGACCAAC ATCTAAATAA CTCCTCCTTT TTTCCTGCTT CAGGTTCTCC TCAAAGAAGT 
ATTGATCAAA GAAGTACAAC TTCAGCTGCT TCTGGTCCTG CAGGCATAGT AACTGTTGTC 
AATGGCGAAT CAAATAGCCT GGCATCTGCT CCCTATCCTC CTGATTCCCT GGTTTCTCAG 
AATGAGAGTG AAGAAGATGA CAATCTAAAT CCAACAGAAA AGCTTCAGAA ACTAAATGAA 
GTTCGTAAGA GGCTGAATGA GTTACGTGAG CTAGTTCACT ACTATGAGCA AACATCTGAT 
ATGATGACAG ATTCTGTGAA TGAAAACACT AAGGAAGAAG AAGAAACCGA AGAATCAGAA 
AGTGATTCTG AACGTGAGGA TCCACAGCCT GTTACAAATA TTAGAAATCC TCAAGGAATC 
AGTAGTTGGA GTGACATAAA TAGCAACTCA AATGTACAGT GTGGAACTAA TAACAGAGAT 
GGAAGACATC TTAATACAGA CTGTGAAATA AATAACAGAT CTGCTGCTAA CATAAGGACT 
CTAAAAATGT CGTCACCTTT AGACTGTCAT AATAGGGAGG ATGACAAAGA TATTGGCCTA 
CCCCAATGTG AAGATGAAGA AGTGGAAGAA GATAGAGTTA GTGAAGATTC TGCATCTAGT 
CACAGAAGCA GCCTGGGTGA TACTGCTGGA GATGCTGAGT TTGAGCAGAA GATCAATAGG 
CTTATAGCTG CAAAACAGAA ACTTAGACAA TTACAAAACC TTGCTGCTAT GGTGCAGGAT 
GATGATCCAG CACCTCAGAC AACAATTGCA AATGCATCTA ATATTGGTGA CTTGTTTTTG 
GGTGAGATGG AAGAGACAAA GCAGCAGCCA AACAATGTCC GAGCTAGTAC CAACAAATTA 
CAAAAAGATG TAGAACTGAA TGAAAAAGCA AGAGAGAAGT TTTATGAAGC TAAACTCCAG 
CAGCAGCAAC AGGAGCTTAA GCAGTTACAA GAAGAAAGAA GAAAACTGAT TGAAATTCAA 
GAAAAAATTC AGGTGTTACA GAAAGCTTGT CCTGATCTTC AATTGTCAGC TGGCTTGGGT 
GACTGCACAG CAAATAGACA GACTTCACCA GCAACATCAA CTCCAGCCAT GAATGACACA 
GCTGGCAAGC CTTCACTTGA ATTTGATGAA TCTGTACCAG TAGGCAATGA GTTATGGTCT 
GAGATGAGAA GACATGAGAT TTTAAGAGAA GAATTGCGAC AAAGAAGAAA GCAACTGGAA 
GCTCTAATGG CTGAGCATCA GAGGAGGAAA GAGCTCGCAG AAACAATATC TACTGTTGCT 
GCGTCTGTTA AAAGTGAAGG ATCAGAAGCT CGGCGTACTC CACAGCAGAG TAGGACTGAA 
AAGACAATGG CTACCTGGGG AGGTTCTACG CAGTGTGCAC TAGATGAAGA GGATGGAGAT 
GAAGATGGTT ATCTCTCTGA TGGAGCTGGT CAGGCAGAAG AAGATGATGA TGATGCGTCA 
AGTGCGAATG ACAGCTTCTC TGTTTATCCC AATAACAACG TACCAGAAAA TGCATATTTT 
GTTAAAGAAA ACAAGGATAG GTGGAAAAAT TACCGTCCTC TTTCAGCAGA TGGAAATTAT 
CGTCCATTGT CTAAGGCCAG ACAACAGCAA AACATAAGTA TGCGACGTCA GGAAAACCTT 
CGGTGGATGT CTGAACTTTC ATATGTGGAA GAAAAGGAAC GATGGCAAGA GCAGATCAAT 
CAGTTGAAGA AACAGCATGA ATTTAGTGTC AGCATTTGTC AAACTTTAAT GCAGGATCAG 
CAGACCCTCT CTTGTCTTCT ACAGACTTTG CTTACAGGCC CTTACAGTAT GATGCCCAAT 
AATGTTGCAT CTTCACAAGT ACATCTTATT ATGCATCAGC TAAACCAGTG TTACACTCAA 
CTGACATGGC AGCAGAATAA CGTCCAGAGG TTGAAACAAA TGTTAAATGA TCTTGTGCGT 
CAGCAAGAAG AACAACGGTA TGAAGAGAAG TCATCAAGAA AGGATAGAGG CAGTGGTGTA 
CCCCCACCTC CATCTCCTGT TTTCTGTCCG TTCAGCTTTC CTCCACAACC TTTGAACCTG 
TTTAATGTAC CAGGATTTAC TAATTTTTCT TCCTTTGCTC CAGATATTAA CTATAATCCA 
GTGTTTCCTT CTGGTTTTGG AGATTTTGCA CACAATATTT CTCCACACAG CAGTGAGCAG 
CAGCAGCAAC ATCCTCTAGA TCATAATGCA TCTGGGAAAA CTGAGTATAT GGCGTTCCCC 
AAACCTTTTG AAAGCAGTTC CTCTAATGGA GCGGAAAAAC AAAGGAGGAG TCACAGACAA 
CCTGAAGAGG AAATGGAAAA AAGATCAGCT TGGCTTAATG ATAGCCAGGA CGTGAAAAAA 
GATGATCAGT CTCAGCTGAA AGCAAGTTTT GCTGTTTCAG TACAAAACAT TGCTTCCAAT 
CATAAAAATC AGTCTGATAT GAGCAGAAGA AGAGAGTTTG ATGAAGAGTC TTTGGAGAGT 
TTTAGTAGCA TGCCTGATCC AGTAGACCCC ACTACTGTGA CAAAGACATT TAAATCTAGA 
AAAGCATCAG CACAAGCAAG TTTGGCATCA AAAGATAAAA CACCTAAATC GAAGAATAAG 
AGGAAGAGTT CTTCTCAGCT AAAAGGCAGA ATTACAAATA TTGATTATGA AAGTGCCAGT 
GCTTCTAGTG TGTGTGAACC CTGCAAGAGC AATAAAAGCA GACACTCTGA AGAGGTTGGT 
CATGCAAAGG TGTTCAGCAA AAGGAATCGG GAACAACTGG AAAAAATCAT TAAATACAGT 
AGATCTACAG AAATGTCTTC AGCGCATGCT AGGAGAATTC TGCAGCAGTC TAACAGAAAT 
GCATGCATTG AAGCGCCAGA AACTGGTAGT GATCTTTCTA TGTTTGAAGC TTTACGAGAC 
ACAATTTATT CTGAAGTGGC AACTCTTATT TCTCAAAATG AGTCTCGTCC CCACTTTCTT 
ATTGAACTTT TCCATGAGCT TCAGCTGCTA AATACAGATT ACCTGAGGCA AAGAGCTCTA 
TACGCTCTAC AGGATATAGT GACCAGACAT TTATCTGAGA ACAATGAAAA AGGGAAGTGC 
ACAAAATCAC TGAATTCTGC AGCATGGATA GCATCAAATT CTGAACTCAC TCCCAGTGAA 
AGCCTTGCTT CTACAGATGA TGAAACTTTT GGCAAAAACT TTTCTACAGA AGCATGTCAA 
GATTGTGAAC AAAATGATGC AGACAATGGG AGTAGCATGT CTACATCTTC AAATTTTGAA 
CCCTTTGCCA CTGATGACCT TGGCAACACA GTGATTCACT TAGATCAAGC TTTGGCTAGG 
ATGAGGGAGT ATGAGCGTAT GAAAATTGAA GCTGAAAGTA CCCTTGACTC TGAGGGCTGC 
TCTAGTAATT TTCAGGGTGC TTCTGCTGCT AAATTAGAAG GAGGTCAAAG TGCCAGTGAA 
TGTGTTCCTG TGCCACAGTC AAGTGAAGCT TCTGCTGTTC CATGTCCTCA TATAGACACT 
CAGCAGCTTG ACCGGCAGAT TAAAGCAATT ATGAAAGAGG TCATTCCTTT TCTGAAGGAA 
CACATGGATG AAGTATGCTC TTCGCAGTTA CTGACATCAG TAAGACGTAT GGTCTTGACC 
CTTACACAAC AAAATGATGA AAGTAAAGAG TTTGTGAAGT TCTTTCATAA GCAGCTTGGC 
AGTATACTTC AGGATTCACT GGCAAAATTT GCTGGTAGAA AACTGAAAGA TTGTGGAGAG 
GATCTTCTAG TGGAGATCTC TGAAGTGTTA TTTAATGAAT TAGCCTTTTT TAAACTCATG 
CAAGACTTGG ACAACAACAG TATTTCTGTA AAGCAGAGAT GTAAAAGAAA AATAGAAGCT 
ACTGAAGTGA TACAGTCGTA TGCTAAAGAG GCAAAAAAAG GTCTCCAGGT GGATGTTTGT 
TCATCTGCTG AAGATGTCGA TGAGGACAAA GACAAGGATG AGACTGAAAC TGTTAAACAA 
GTACAGGACT CAGAAATGTA TGATGGGAAT AGAGTTCCTG AAAGTATTAG GTCTGATGCA 
TCTGATCAGG AGGAAGATGA TGAAAGTGAA AGCGGTCCAG TGGCAATAAG TTTATCAAAA 
GCAGAAACCC AAGCTCTGAC TAACTATGGT AGTGGAGAAG ATGAGAATGA AGATGAAGAA 
ATAGAATTTG AGGAAGGACC TGTTGATGTG CAGACGTCAT TACAAGCCAG CAGTGAAACA 
ACAACTGAAA ATGAACAGAA TTCAAACCAA GAACAAAGTA AGACAAAAAG CAGTGAGATT 
CTGTCATCAG AACAAGAATC TGTTTATGCT AAAGGTGAAC AACATGTGGC TACAATTTTG 
CCTTATTACC TCAATGTAAT GGAGAATGCA CCGCCTTTAA CGGTCAATAC CCCAGAATCT 
TTTATAACAG ACAGTATGAA AACAGAAGAA TCAAGTTCAT CTTTACCAGT AAATGAAACT 
CAAACACTGG ATACAACGTG TGTAGGAAAC AAATCTGCTG CAAGTTCTGA AAGCTCCATG 
GCTGGCAGCC CTGATACAGA GTCACCTGTG TTAGTGAATG AATATGAAGC TGGTTCTGGA 
AATGTAAGTC AAAGATCTGA TGAAGATGAC TTTGTGAAAG TTGAAGACTT GCCCCTTAAA 
CTTGCAGTGT ATTCAGAGGC AGATTTAATG AAGAAAATGG CAATGGAGGC CCAAACGAAC 
AGTTTGTCTG ATGAATTACT GGGTGGAGGT GGAGCTCAAG ATCAAGAATT AGTAGGAGAT 
GCCCAAACTT TGAAAGAACC TGAAACTTTT GGAGTTCAAA GTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021141546.1
CDS	140..6367
Translation	MATGGGPFEEGMNDQDLPSWSNDSFDDRLNNTDWGSQQKKANRSSEKNKKKLGGEAETRLTNDISPESSPGIGRRKTRTPHSFPHARYMTQMSVPEQAELERLKQRINFSDLDQRSIGSDSQGRATAANNKRQLNENKKPFNFLSLQINTNKSKDPASCSQKKESGVSVQRKELFGAALSKDFLQNCQVSAQEDGGGEPAMDSSQIVSRLVQIRDYIAKASSMRDDLVEKNERSANVERLSHLIDHLKEQEKSYLKFLQKMLARQNEEDDVRTIDSAVGSGSVGESTSLNIDVQSEASDTTARDPQQEAKEELENLKKQHDLLKRMLKQQEQLKALQGRQAALLALQHKAEQAIAVMDDSVVTETTGSVSGVSLTSELNEELNDLIQRFHNQLHDSQTQSVPDNRRQAESLSLTREVSQSRNSSMSERLSDEKAQLFNKMRMLQGKKQKMDKLLGELHTLRDQHLNNSSFFPASGSPQRSIDQRSTTSAASGPAGIVTVVNGESNSLASAPYPPDSLVSQNESEEDDNLNPTEKLQKLNEVRKRLNELRELVHYYEQTSDMMTDSVNENTKEEEETEESESDSEREDPQPVTNIRNPQGISSWSDINSNSNVQCGTNNRDGRHLNTDCEINNRSAANIRTLKMSSPLDCHNREDDKDIGLPQCEDEEVEEDRVSEDSASSHRSSLGDTAGDAEFEQKINRLIAAKQKLRQLQNLAAMVQDDDPAPQTTIANASNIGDLFLGEMEETKQQPNNVRASTNKLQKDVELNEKAREKFYEAKLQQQQQELKQLQEERRKLIEIQEKIQVLQKACPDLQLSAGLGDCTANRQTSPATSTPAMNDTAGKPSLEFDESVPVGNELWSEMRRHEILREELRQRRKQLEALMAEHQRRKELAETISTVAASVKSEGSEARRTPQQSRTEKTMATWGGSTQCALDEEDGDEDGYLSDGAGQAEEDDDDASSANDSFSVYPNNNVPENAYFVKENKDRWKNYRPLSADGNYRPLSKARQQQNISMRRQENLRWMSELSYVEEKERWQEQINQLKKQHEFSVSICQTLMQDQQTLSCLLQTLLTGPYSMMPNNVASSQVHLIMHQLNQCYTQLTWQQNNVQRLKQMLNDLVRQQEEQRYEEKSSRKDRGSGVPPPPSPVFCPFSFPPQPLNLFNVPGFTNFSSFAPDINYNPVFPSGFGDFAHNISPHSSEQQQQHPLDHNASGKTEYMAFPKPFESSSSNGAEKQRRSHRQPEEEMEKRSAWLNDSQDVKKDDQSQLKASFAVSVQNIASNHKNQSDMSRRREFDEESLESFSSMPDPVDPTTVTKTFKSRKASAQASLASKDKTPKSKNKRKSSSQLKGRITNIDYESASASSVCEPCKSNKSRHSEEVGHAKVFSKRNREQLEKIIKYSRSTEMSSAHARRILQQSNRNACIEAPETGSDLSMFEALRDTIYSEVATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVTRHLSENNEKGKCTKSLNSAAWIASNSELTPSESLASTDDETFGKNFSTEACQDCEQNDADNGSSMSTSSNFEPFATDDLGNTVIHLDQALARMREYERMKIEAESTLDSEGCSSNFQGASAAKLEGGQSASECVPVPQSSEASAVPCPHIDTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDESKEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFKLMQDLDNNSISVKQRCKRKIEATEVIQSYAKEAKKGLQVDVCSSAEDVDEDKDKDETETVKQVQDSEMYDGNRVPESIRSDASDQEEDDESESGPVAISLSKAETQALTNYGSGEDENEDEEIEFEEGPVDVQTSLQASSETTTENEQNSNQEQSKTKSSEILSSEQESVYAKGEQHVATILPYYLNVMENAPPLTVNTPESFITDSMKTEESSSSLPVNETQTLDTTCVGNKSAASSESSMAGSPDTESPVLVNEYEAGSGNVSQRSDEDDFVKVEDLPLKLAVYSEADLMKKMAMEAQTNSLSDELLGGGGAQDQELVGDAQTLKEPETFGVQSA

Target ORF information:

RefSeq Version	XM_021285871.1
Organism	Columba livia(rock pigeon)
Definition	Columba livia pericentriolar material 1 (PCM1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021285871.1

ORF Insert Sequence:

ATGGCAACAG GAGGTGGTCC CTTTGAAGAA GGCATGAATG ATCAAGACTT GCCTAGCTGG 
AGCAATGACA GCTTTGATGA CCGGCTGAAC AACACGGACT GGGGAAGTCA ACAGAAGAAA 
GCAAACAGAT CTTCAGAAAA AAACAAGAAA AAGCTTGGTG GGGAAGCTGA AACAAGGCTT 
ACTAATGATA TATCTCCAGA ATCCTCACCT GGAATAGGAC GACGAAAGAC CAGAACTCCT 
CATAGTTTTC CTCATGCTCG ATACATGACC CAGATGTCTG TTCCGGAGCA GGCTGAGCTA 
GAAAGGCTTA AACAAAGAAT AAACTTCAGT GATCTGGATC AGAGAAGCAT TGGAAGTGAT 
TCTCAAGGCA GGGCAACAGC TGCTAACAAC AAACGTCAAC TTAATGAAAA CAAAAAACCA 
TTCAACTTCC TGTCACTGCA GATAAACACT AACAAGAGCA AAGATCCTGC CTCATGTTCC 
CAAAAAAAGG AAAGTGGGGT GTCAGTCCAA CGTAAAGAGT TGTTTGGTGC TGCTCTGAGC 
AAGGATTTCT TGCAAAATTG TCAAGTCTCT GCTCAAGAAG ATGGAGGGGG AGAACCAGCT 
ATGGATAGTA GCCAGATTGT GAGCAGACTA GTTCAAATTC GCGACTATAT TGCTAAGGCC 
AGCTCCATGC GGGATGATCT TGTAGAGAAA AATGAAAGAT CGGCCAATGT TGAGCGTTTA 
TCACACCTTA TAGATCACCT TAAAGAGCAG GAGAAATCCT ATCTGAAATT TTTGCAAAAG 
ATGCTTGCTA GACAAAATGA GGAGGATGAT GTTCGGACTA TAGATTCAGC TGTGGGATCT 
GGTTCTGTAG GTGAGAGCAC ATCGCTAAAC ATTGATGTGC AGTCTGAGGC TTCAGATACC 
ACGGCTAGAG ATCCTCAACA GGAGGCTAAA GAGGAGTTGG AGAACTTGAA GAAGCAGCAT 
GATTTATTGA AAAGGATGCT AAAACAGCAA GAGCAATTAA AGGCTCTTCA AGGAAGACAG 
GCAGCTCTTC TTGCTTTGCA GCATAAAGCA GAGCAAGCAA TTGCTGTCAT GGATGATTCT 
GTTGTAACAG AGACTACAGG TAGTGTTTCA GGAGTAAGCC TTACATCAGA ACTGAATGAA 
GAATTGAATG ACTTAATTCA GCGCTTTCAC AACCAACTTC ATGATTCTCA GACACAGTCT 
GTACCAGACA ATAGAAGGCA AGCAGAAAGT CTTTCACTTA CTAGAGAGGT TTCACAAAGC 
AGAAACTCTT CAATGTCTGA ACGCCTGTCA GATGAGAAGG CGCAGCTTTT TAACAAGATG 
CGAATGTTGC AGGGTAAAAA GCAAAAAATG GACAAACTGT TAGGAGAACT TCATACACTT 
CGTGACCAAC ATCTAAATAA CTCCTCCTTT TTTCCTGCTT CAGGTTCTCC TCAAAGAAGT 
ATTGATCAAA GAAGTACAAC TTCAGCTGCT TCTGGTCCTG CAGGCATAGT AACTGTTGTC 
AATGGCGAAT CAAATAGCCT GGCATCTGCT CCCTATCCTC CTGATTCCCT GGTTTCTCAG 
AATGAGAGTG AAGAAGATGA CAATCTAAAT CCAACAGAAA AGCTTCAGAA ACTAAATGAA 
GTTCGTAAGA GGCTGAATGA GTTACGTGAG CTAGTTCACT ACTATGAGCA AACATCTGAT 
ATGATGACAG ATTCTGTGAA TGAAAACACT AAGGAAGAAG AAGAAACCGA AGAATCAGAA 
AGTGATTCTG AACGTGAGGA TCCACAGCCT GTTACAAATA TTAGAAATCC TCAAGGAATC 
AGTAGTTGGA GTGACATAAA TAGCAACTCA AATGTACAGT GTGGAACTAA TAACAGAGAT 
GGAAGACATC TTAATACAGA CTGTGAAATA AATAACAGAT CTGCTGCTAA CATAAGGACT 
CTAAAAATGT CGTCACCTTT AGACTGTCAT AATAGGGAGG ATGACAAAGA TATTGGCCTA 
CCCCAATGTG AAGATGAAGA AGTGGAAGAA GATAGAGTTA GTGAAGATTC TGCATCTAGT 
CACAGAAGCA GCCTGGGTGA TACTGCTGGA GATGCTGAGT TTGAGCAGAA GATCAATAGG 
CTTATAGCTG CAAAACAGAA ACTTAGACAA TTACAAAACC TTGCTGCTAT GGTGCAGGAT 
GATGATCCAG CACCTCAGAC AACAATTGCA AATGCATCTA ATATTGGTGA CTTGTTTTTG 
GGTGAGATGG AAGAGACAAA GCAGCAGCCA AACAATGTCC GAGCTAGTAC CAACAAATTA 
CAAAAAGATG TAGAACTGAA TGAAAAAGCA AGAGAGAAGT TTTATGAAGC TAAACTCCAG 
CAGCAGCAAC AGGAGCTTAA GCAGTTACAA GAAGAAAGAA GAAAACTGAT TGAAATTCAA 
GAAAAAATTC AGGTGTTACA GAAAGCTTGT CCTGATCTTC AATTGTCAGC TGGCTTGGGT 
GACTGCACAG CAAATAGACA GACTTCACCA GCAACATCAA CTCCAGCCAT GAATGACACA 
GCTGGCAAGC CTTCACTTGA ATTTGATGAA TCTGTACCAG TAGGCAATGA GTTATGGTCT 
GAGATGAGAA GACATGAGAT TTTAAGAGAA GAATTGCGAC AAAGAAGAAA GCAACTGGAA 
GCTCTAATGG CTGAGCATCA GAGGAGGAAA GAGCTCGCAG AAACAATATC TACTGTTGCT 
GCGTCTGTTA AAAGTGAAGG ATCAGAAGCT CGGCGTACTC CACAGCAGAG TAGGACTGAA 
AAGACAATGG CTACCTGGGG AGGTTCTACG CAGTGTGCAC TAGATGAAGA GGATGGAGAT 
GAAGATGGTT ATCTCTCTGA TGGAGCTGGT CAGGCAGAAG AAGATGATGA TGATGCGTCA 
AGTGCGAATG ACAGCTTCTC TGTTTATCCC AATAACAACG TACCAGAAAA TGCATATTTT 
GTTAAAGAAA ACAAGGATAG GTGGAAAAAT TACCGTCCTC TTTCAGCAGA TGGAAATTAT 
CGTCCATTGT CTAAGGCCAG ACAACAGCAA AACATAAGTA TGCGACGTCA GGAAAACCTT 
CGGTGGATGT CTGAACTTTC ATATGTGGAA GAAAAGGAAC GATGGCAAGA GCAGATCAAT 
CAGTTGAAGA AACAGCATGA ATTTAGTGTC AGCATTTGTC AAACTTTAAT GCAGGATCAG 
CAGACCCTCT CTTGTCTTCT ACAGACTTTG CTTACAGGCC CTTACAGTAT GATGCCCAAT 
AATGTTGCAT CTTCACAAGT ACATCTTATT ATGCATCAGC TAAACCAGTG TTACACTCAA 
CTGACATGGC AGCAGAATAA CGTCCAGAGG TTGAAACAAA TGTTAAATGA TCTTGTGCGT 
CAGCAAGAAG AACAACGGTA TGAAGAGAAG TCATCAAGAA AGGATAGAGG CAGTGGTGTA 
CCCCCACCTC CATCTCCTGT TTTCTGTCCG TTCAGCTTTC CTCCACAACC TTTGAACCTG 
TTTAATGTAC CAGGATTTAC TAATTTTTCT TCCTTTGCTC CAGATATTAA CTATAATCCA 
GTGTTTCCTT CTGGTTTTGG AGATTTTGCA CACAATATTT CTCCACACAG CAGTGAGCAG 
CAGCAGCAAC ATCCTCTAGA TCATAATGCA TCTGGGAAAA CTGAGTATAT GGCGTTCCCC 
AAACCTTTTG AAAGCAGTTC CTCTAATGGA GCGGAAAAAC AAAGGAGGAG TCACAGACAA 
CCTGAAGAGG AAATGGAAAA AAGATCAGCT TGGCTTAATG ATAGCCAGGA CGTGAAAAAA 
GATGATCAGT CTCAGCTGAA AGCAAGTTTT GCTGTTTCAG TACAAAACAT TGCTTCCAAT 
CATAAAAATC AGTCTGATAT GAGCAGAAGA AGAGAGTTTG ATGAAGAGTC TTTGGAGAGT 
TTTAGTAGCA TGCCTGATCC AGTAGACCCC ACTACTGTGA CAAAGACATT TAAATCTAGA 
AAAGCATCAG CACAAGCAAG TTTGGCATCA AAAGATAAAA CACCTAAATC GAAGAATAAG 
AGGAAGAGTT CTTCTCAGCT AAAAGGCAGA ATTACAAATA TTGATTATGA AAGTGCCAGT 
GCTTCTAGTG TGTGTGAACC CTGCAAGAGC AATAAAAGCA GACACTCTGA AGAGGTTGGT 
CATGCAAAGG TGTTCAGCAA AAGGAATCGG GAACAACTGG AAAAAATCAT TAAATACAGT 
AGATCTACAG AAATGTCTTC AGCGCATGCT AGGAGAATTC TGCAGCAGTC TAACAGAAAT 
GCATGCATTG AAGCGCCAGA AACTGGTAGT GATCTTTCTA TGTTTGAAGC TTTACGAGAC 
ACAATTTATT CTGAAGTGGC AACTCTTATT TCTCAAAATG AGTCTCGTCC CCACTTTCTT 
ATTGAACTTT TCCATGAGCT TCAGCTGCTA AATACAGATT ACCTGAGGCA AAGAGCTCTA 
TACGCTCTAC AGGATATAGT GACCAGACAT TTATCTGAGA ACAATGAAAA AGGGAAGTGC 
ACAAAATCAC TGAATTCTGC AGCATGGATA GCATCAAATT CTGAACTCAC TCCCAGTGAA 
AGCCTTGCTT CTACAGATGA TGAAACTTTT GGCAAAAACT TTTCTACAGA AGCATGTCAA 
GATTGTGAAC AAAATGATGC AGACAATGGG AGTAGCATGT CTACATCTTC AAATTTTGAA 
CCCTTTGCCA CTGATGACCT TGGCAACACA GTGATTCACT TAGATCAAGC TTTGGCTAGG 
ATGAGGGAGT ATGAGCGTAT GAAAATTGAA GCTGAAAGTA CCCTTGACTC TGAGGGCTGC 
TCTAGTAATT TTCAGGGTGC TTCTGCTGCT AAATTAGAAG GAGGTCAAAG TGCCAGTGAA 
TGTGTTCCTG TGCCACAGTC AAGTGAAGCT TCTGCTGTTC CATGTCCTCA TATAGACACT 
CAGCAGCTTG ACCGGCAGAT TAAAGCAATT ATGAAAGAGG TCATTCCTTT TCTGAAGGAA 
CACATGGATG AAGTATGCTC TTCGCAGTTA CTGACATCAG TAAGACGTAT GGTCTTGACC 
CTTACACAAC AAAATGATGA AAGTAAAGAG TTTGTGAAGT TCTTTCATAA GCAGCTTGGC 
AGTATACTTC AGGATTCACT GGCAAAATTT GCTGGTAGAA AACTGAAAGA TTGTGGAGAG 
GATCTTCTAG TGGAGATCTC TGAAGTGTTA TTTAATGAAT TAGCCTTTTT TAAACTCATG 
CAAGACTTGG ACAACAACAG TATTTCTGTA AAGCAGAGAT GTAAAAGAAA AATAGAAGCT 
ACTGAAGTGA TACAGTCGTA TGCTAAAGAG GCAAAAAAAG GTCTCCAGGT GGATGTTTGT 
TCATCTGCTG AAGATGTCGA TGAGGACAAA GACAAGGATG AGACTGAAAC TGTTAAACAA 
GTACAGGACT CAGAAATGTA TGATGGGAAT AGAGTTCCTG AAAGTATTAG GTCTGATGCA 
TCTGATCAGG AGGAAGATGA TGAAAGTGAA AGCGGTCCAG TGGCAATAAG TTTATCAAAA 
GCAGAAACCC AAGCTCTGAC TAACTATGGT AGTGGAGAAG ATGAGAATGA AGATGAAGAA 
ATAGAATTTG AGGAAGGACC TGTTGATGTG CAGACGTCAT TACAAGCCAG CAGTGAAACA 
ACAACTGAAA ATGAACAGAA TTCAAACCAA GAACAAAGTA AGACAAAAAG CAGTGAGATT 
CTGTCATCAG AACAAGAATC TGTTTATGCT AAAGGTGAAC AACATGTGGC TACAATTTTG 
CCTTATTACC TCAATGTAAT GGAGAATGCA CCGCCTTTAA CGGTCAATAC CCCAGAATCT 
TTTATAACAG ACAGTATGAA AACAGAAGAA TCAAGTTCAT CTTTACCAGT AAATGAAACT 
CAAACACTGG ATACAACGTG TGTAGGAAAC AAATCTGCTG CAAGTTCTGA AAGCTCCATG 
GCTGGCAGCC CTGATACAGA GTCACCTGTG TTAGTGAATG AATATGAAGC TGGTTCTGGA 
AATGTAAGTC AAAGATCTGA TGAAGATGAC TTTGTGAAAG TTGAAGACTT GCCCCTTAAA 
CTTGCAGTGT ATTCAGAGGC AGATTTAATG AAGAAAATGG CAATGGAGGC CCAAACGAAC 
AGTTTGTCTG ATGAATTACT GGGTGGAGGT GGAGCTCAAG ATCAAGAATT AGTAGGAGAT 
GCCCAAACTT TGAAAGAACC TGAAACTTTT GGAGTTCAAA GTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	PCM1
Entrez Gene ID	102098946
Full Name	pericentriolar material 1
Gene Type	protein-coding
Organism	Columba livia(rock pigeon)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

PCM1 cDNA ORF clone, Columba livia(rock pigeon)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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