DHX8 cDNA ORF clone, Colius striatus(speckled mousebird)

Gene
Clones
gRNAs

Summary

Gene Symbol	DHX8
Entrez Gene ID	104553021
Full Name	DEAH-box helicase 8
Gene Type	protein-coding
Organism	Colius striatus(speckled mousebird)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010195501.1	XP_010193803.1	ATP-dependent RNA helicase DHX8

Pathways from BioSystems
Gene Ontology

The following DHX8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DHX8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCo159208	XM_010195501.1 Latest version!	Colius striatus DEAH (Asp-Glu-Ala-His) box polypeptide 8 (DHX8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DHX8 ( XM_010195501.1 ) cDNA ORF clone, Colius striatus(speckled mousebird) -> XP_010193803.1 Colius striatus DEAH (Asp-Glu-Ala-His) box polypeptide 8 (DHX8), mRNA.

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CloneID	OCo159208
Clone ID Related Accession (Same CDS sequence)	XM_010195501.1
Accession Version	XM_010195501.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-06
Organism	Colius striatus(speckled mousebird)
Product	ATP-dependent RNA helicase DHX8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010671691.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Colius striatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACCTC CACCAAAACC GTCTACAAGC AAAGAGACAG CTGTCAAACC CAAATCAGAG 
AAGGAAAAAT TGAAGGAATT GTTTCCAGCC CTTTGCAGGC CAGACAATCC CAACATCAGG 
AATATGCTGG ATGAAGATGA TGTGAAGGTG GCAGCTGATG CACTGAAAGA GCTTGAAGCT 
CTGATGCCTA GTGCAGACAA GCACGACAAG CAAAGGAGCA GTGATCACCG GGCAAAGAAA 
AGGAAGAGGA GCCGAAGTCG TAGCAGGGAC AGAAAGAGAA GGCACAGATC TCGCTCAAGG 
TCTCATTCTA GGACTCGGGA TAGGAACAGG GGAAAATCCA GGTACAGGTC AAGGAGCAGA 
AGTCGGAGTC CCAGTAGGGA CCGTAAGGAG CGAGAGAAAT ACTCTGAGAA GAGCAGTGAG 
AGATGGAGAG ACAAGCACAT AGACCGCCCA CCACCCGAGG AGCCTTCCAT TGGAGACATT 
TACAATGGCA AAGTCACAAG TATAATGCAG TTTGGATGCT TCGTGCAGCT GGAAGGACTC 
AGGAAGCGTT GGGAGGGCTT GGTCCACATC TCGGAGCTTC GACGAGAGGG TCGCGTTGCC 
AATGTCGCTG ACGTTGTGAG CAAAGGCCAA AGAGTAAAAG TCAAAGTGTT ATCCTTCACT 
GGATCCAAAA CCAGCCTGAG CATGAAGGAT GTTGATCAAG ACACTGGGGA AGACTTGAAC 
CCAAACCGCA GGAGAAACCT GGTTGGAGAA ACCAATGAAG AAATCTCCAT GAGAAACCCA 
GACAGACCCA GTCACCTGTC CCTGGTGAAT GCCCCAGAGG TAGAGGATGA CAGCCTGGAA 
AGGAAGCGCC TCACCAGAAT CTCAGACCCA GAGAAATGGG AAATAAAGCA GATGATTGCA 
GCTAATGTGC TTTCCAAGGA AGAGTTTCCT GACTTTGATG AGGAGACTGG GATTCTTCCT 
AAAGTTGATG ATGAAGAAGA TGAGGACCTT GAGATTGAGT TGGTGGAAGA AGAGCCACCG 
TTCCTTCGAG GTCATACTAA ACAGAGCATG GATATGAGTC CCATCAAAAT AGTAAAGAAT 
CCAGATGGTT CCTTGTCCCA GGCTGCAATG ATGCAGAGTG CTTTAGCTAA AGAAAGACGA 
GAACTTAAAC AGGCTCAGAG AGAAGCAGAA ATGGATTCTA TCCCCATGGG ACTCAACACA 
CACTGGGTGG ACCCCCTCCC TGATGTGGAT GGAAGGCAAA TAGCCGCAAA CATGAGAGGT 
ATTGGGATGA TGCCCAATGA TATCCCAGAG TGGAAGAAAC ATGCCTTTGG TGGCAACAAA 
GCTTCTTATG GTAAGAAGAC CCAGCTTTCC ATCATTGAGC AGAGAGAGAG TCTCCCAATC 
TTCAGACTGA AGGAGCAGCT GATACAAGCT GTGCATGACA ACCAGATCCT GATTGTTATT 
GGAGAGACAG GATCTGGGAA GACAACACAG ATTACCCAGT ACCTGGCTGA AGCAGGCTAC 
ACGTCAAGAG GAAAGATTGG ATGCACTCAG CCTCGCAGAG TGGCTGCAAT GTCTGTTGCA 
AAGAGGGTGT CAGAGGAGTT TGGTTGCTGC TTGGGACAAG AGGTTGGCTA CACCATTCGA 
TTTGAAGACT GCACTAGCCC TGAAACTGTT ATCAAATACA TGACAGATGG TATGTTACTG 
AGGGAGTGCT TGATAGACCC TGATCTGACC CAGTATGCCA TTATCATGCT GGATGAAGCC 
CACGAGAGGA CCATACATAC CGATGTGCTC TTTGGACTCC TCAAGAAGAC AGTGCAGAAG 
CGACAGGATA TGAAGCTGAT AGTGACATCA GCAACGCTGG ATGCCGTGAA GTTCTCTCAA 
TACTTTTATG AAGCCCCAAT CTTCACAATT CCTGGCAGAA CATACCCAGT AGAAATTCTG 
TACACAAAAG AGCCAGAGAC AGATTATTTG GATGCAAGTC TGATTACAGT AATGCAGATC 
CACTTAACAG AGCCACCAGG TGACATTCTG GTGTTTCTGA CTGGTCAGGA AGAGATTGAC 
ACTGCCTGTG AAATCCTTTA TGAGAGGATG AAATCTCTAG GACCTGATGT TCCAGAGTTA 
ATTATCCTAC CAGTATATTC AGCTTTACCC AGTGAAATGC AAACCAGGAT CTTTGACCCA 
GCCCCACCAG GAAGCAGAAA GGTTGTCATT GCCACCAACA TTGCTGAGAC ATCTCTGACT 
ATTGATGGGA TATATTATGT AGTGGATCCT GGCTTTGTAA AGCAGAAAGT TTACAATTCC 
AAGACTGGAA TTGACCAGCT GGTTGTTACA CCCATTTCAC AGGCTCAAGC AAAGCAACGA 
GCAGGAAGGG CTGGGAGAAC AGGACCAGGA AAATGCTATA GGTTGTACAC AGAGCGTGCT 
TATCGAGATG AAATGCTGAC CACCAATGTG CCTGAAATCC AGAGAACAAA TTTGGCTAGT 
ACAGTGCTTT CCCTGAAGGC TATGGGCATC AATGATTTAC TCTCCTTTGA CTTTATGGAT 
GCTCCCCCAA TGGAAACTCT CATCACTGCC ATGGAGCAGC TGTACACCCT GGGGGCTCTG 
GATGATGAGG GACTTCTCAC TCGACTTGGG CGCAGGATGG CAGAATTCCC CTTGGAGCCT 
ATGTTGTGTA AGATGTTGAT CATGTCTGTG CACCTGGGAT GCAGCGAGGA AATGCTGACA 
ATAGTCTCCA TGCTCTCTGT ACAGAATGTA TTCTACAGGC CAAAGGATAA ACAAGCACTT 
GCTGATCAGA AGAAAGCCAA GTTCCATCAG ACAGAAGGTG ACCACCTCAC TCTTCTGGCT 
GTCTACAATT CCTGGAAGAA TAATAAGTTT TCAAATCCCT GGTGCTATGA AAATTTCATT 
CAGGCTCGAT CCTTACGCAG GGCACAGGAC ATCCGCAAGC AGATGTTGGG CATTATGGAC 
AGGCACAAGC TGGATGTGGT ATCCTGTGGG AAGGCAACGG TTCGTGTCCA GAAGGCCATT 
TGTAGCGGCT TCTTCCGAAA CGCGGCAAAG AAGGATCCTC AGGAGGGTTA TCGGACACTC 
ATTGATCAAC AAGTGGTTTA TATCCACCCA TCCAGTGCTC TCTTCAACAG GCAGCCAGAG 
TGGGTGGTGT ATCACGAGCT GGTCCTGACC ACCAAGGAAT ACATGCGTGA GGTGACGACT 
ATCGATCCTC GCTGGCTGGT GGAATTTGCA CCAGCTTTCT TCAAGGTTTC TGATCCAACC 
AAGCTGAGCA AACAGAAGAA ACAGCAGCGT CTGGAACCCT TGTACAATCG CTACGAGGAG 
CCCAATGCTT GGAGGATATC TCGTGCCTTC AGACGACGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010193803.1
CDS	220..3561
Translation	MRPPPKPSTSKETAVKPKSEKEKLKELFPALCRPDNPNIRNMLDEDDVKVAADALKELEALMPSADKHDKQRSSDHRAKKRKRSRSRSRDRKRRHRSRSRSHSRTRDRNRGKSRYRSRSRSRSPSRDRKEREKYSEKSSERWRDKHIDRPPPEEPSIGDIYNGKVTSIMQFGCFVQLEGLRKRWEGLVHISELRREGRVANVADVVSKGQRVKVKVLSFTGSKTSLSMKDVDQDTGEDLNPNRRRNLVGETNEEISMRNPDRPSHLSLVNAPEVEDDSLERKRLTRISDPEKWEIKQMIAANVLSKEEFPDFDEETGILPKVDDEEDEDLEIELVEEEPPFLRGHTKQSMDMSPIKIVKNPDGSLSQAAMMQSALAKERRELKQAQREAEMDSIPMGLNTHWVDPLPDVDGRQIAANMRGIGMMPNDIPEWKKHAFGGNKASYGKKTQLSIIEQRESLPIFRLKEQLIQAVHDNQILIVIGETGSGKTTQITQYLAEAGYTSRGKIGCTQPRRVAAMSVAKRVSEEFGCCLGQEVGYTIRFEDCTSPETVIKYMTDGMLLRECLIDPDLTQYAIIMLDEAHERTIHTDVLFGLLKKTVQKRQDMKLIVTSATLDAVKFSQYFYEAPIFTIPGRTYPVEILYTKEPETDYLDASLITVMQIHLTEPPGDILVFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSALPSEMQTRIFDPAPPGSRKVVIATNIAETSLTIDGIYYVVDPGFVKQKVYNSKTGIDQLVVTPISQAQAKQRAGRAGRTGPGKCYRLYTERAYRDEMLTTNVPEIQRTNLASTVLSLKAMGINDLLSFDFMDAPPMETLITAMEQLYTLGALDDEGLLTRLGRRMAEFPLEPMLCKMLIMSVHLGCSEEMLTIVSMLSVQNVFYRPKDKQALADQKKAKFHQTEGDHLTLLAVYNSWKNNKFSNPWCYENFIQARSLRRAQDIRKQMLGIMDRHKLDVVSCGKATVRVQKAICSGFFRNAAKKDPQEGYRTLIDQQVVYIHPSSALFNRQPEWVVYHELVLTTKEYMREVTTIDPRWLVEFAPAFFKVSDPTKLSKQKKQQRLEPLYNRYEEPNAWRISRAFRRR

Target ORF information:

RefSeq Version	XM_010195501.1
Organism	Colius striatus(speckled mousebird)
Definition	Colius striatus DEAH (Asp-Glu-Ala-His) box polypeptide 8 (DHX8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010195501.1

ORF Insert Sequence:

ATGCGACCTC CACCAAAACC GTCTACAAGC AAAGAGACAG CTGTCAAACC CAAATCAGAG 
AAGGAAAAAT TGAAGGAATT GTTTCCAGCC CTTTGCAGGC CAGACAATCC CAACATCAGG 
AATATGCTGG ATGAAGATGA TGTGAAGGTG GCAGCTGATG CACTGAAAGA GCTTGAAGCT 
CTGATGCCTA GTGCAGACAA GCACGACAAG CAAAGGAGCA GTGATCACCG GGCAAAGAAA 
AGGAAGAGGA GCCGAAGTCG TAGCAGGGAC AGAAAGAGAA GGCACAGATC TCGCTCAAGG 
TCTCATTCTA GGACTCGGGA TAGGAACAGG GGAAAATCCA GGTACAGGTC AAGGAGCAGA 
AGTCGGAGTC CCAGTAGGGA CCGTAAGGAG CGAGAGAAAT ACTCTGAGAA GAGCAGTGAG 
AGATGGAGAG ACAAGCACAT AGACCGCCCA CCACCCGAGG AGCCTTCCAT TGGAGACATT 
TACAATGGCA AAGTCACAAG TATAATGCAG TTTGGATGCT TCGTGCAGCT GGAAGGACTC 
AGGAAGCGTT GGGAGGGCTT GGTCCACATC TCGGAGCTTC GACGAGAGGG TCGCGTTGCC 
AATGTCGCTG ACGTTGTGAG CAAAGGCCAA AGAGTAAAAG TCAAAGTGTT ATCCTTCACT 
GGATCCAAAA CCAGCCTGAG CATGAAGGAT GTTGATCAAG ACACTGGGGA AGACTTGAAC 
CCAAACCGCA GGAGAAACCT GGTTGGAGAA ACCAATGAAG AAATCTCCAT GAGAAACCCA 
GACAGACCCA GTCACCTGTC CCTGGTGAAT GCCCCAGAGG TAGAGGATGA CAGCCTGGAA 
AGGAAGCGCC TCACCAGAAT CTCAGACCCA GAGAAATGGG AAATAAAGCA GATGATTGCA 
GCTAATGTGC TTTCCAAGGA AGAGTTTCCT GACTTTGATG AGGAGACTGG GATTCTTCCT 
AAAGTTGATG ATGAAGAAGA TGAGGACCTT GAGATTGAGT TGGTGGAAGA AGAGCCACCG 
TTCCTTCGAG GTCATACTAA ACAGAGCATG GATATGAGTC CCATCAAAAT AGTAAAGAAT 
CCAGATGGTT CCTTGTCCCA GGCTGCAATG ATGCAGAGTG CTTTAGCTAA AGAAAGACGA 
GAACTTAAAC AGGCTCAGAG AGAAGCAGAA ATGGATTCTA TCCCCATGGG ACTCAACACA 
CACTGGGTGG ACCCCCTCCC TGATGTGGAT GGAAGGCAAA TAGCCGCAAA CATGAGAGGT 
ATTGGGATGA TGCCCAATGA TATCCCAGAG TGGAAGAAAC ATGCCTTTGG TGGCAACAAA 
GCTTCTTATG GTAAGAAGAC CCAGCTTTCC ATCATTGAGC AGAGAGAGAG TCTCCCAATC 
TTCAGACTGA AGGAGCAGCT GATACAAGCT GTGCATGACA ACCAGATCCT GATTGTTATT 
GGAGAGACAG GATCTGGGAA GACAACACAG ATTACCCAGT ACCTGGCTGA AGCAGGCTAC 
ACGTCAAGAG GAAAGATTGG ATGCACTCAG CCTCGCAGAG TGGCTGCAAT GTCTGTTGCA 
AAGAGGGTGT CAGAGGAGTT TGGTTGCTGC TTGGGACAAG AGGTTGGCTA CACCATTCGA 
TTTGAAGACT GCACTAGCCC TGAAACTGTT ATCAAATACA TGACAGATGG TATGTTACTG 
AGGGAGTGCT TGATAGACCC TGATCTGACC CAGTATGCCA TTATCATGCT GGATGAAGCC 
CACGAGAGGA CCATACATAC CGATGTGCTC TTTGGACTCC TCAAGAAGAC AGTGCAGAAG 
CGACAGGATA TGAAGCTGAT AGTGACATCA GCAACGCTGG ATGCCGTGAA GTTCTCTCAA 
TACTTTTATG AAGCCCCAAT CTTCACAATT CCTGGCAGAA CATACCCAGT AGAAATTCTG 
TACACAAAAG AGCCAGAGAC AGATTATTTG GATGCAAGTC TGATTACAGT AATGCAGATC 
CACTTAACAG AGCCACCAGG TGACATTCTG GTGTTTCTGA CTGGTCAGGA AGAGATTGAC 
ACTGCCTGTG AAATCCTTTA TGAGAGGATG AAATCTCTAG GACCTGATGT TCCAGAGTTA 
ATTATCCTAC CAGTATATTC AGCTTTACCC AGTGAAATGC AAACCAGGAT CTTTGACCCA 
GCCCCACCAG GAAGCAGAAA GGTTGTCATT GCCACCAACA TTGCTGAGAC ATCTCTGACT 
ATTGATGGGA TATATTATGT AGTGGATCCT GGCTTTGTAA AGCAGAAAGT TTACAATTCC 
AAGACTGGAA TTGACCAGCT GGTTGTTACA CCCATTTCAC AGGCTCAAGC AAAGCAACGA 
GCAGGAAGGG CTGGGAGAAC AGGACCAGGA AAATGCTATA GGTTGTACAC AGAGCGTGCT 
TATCGAGATG AAATGCTGAC CACCAATGTG CCTGAAATCC AGAGAACAAA TTTGGCTAGT 
ACAGTGCTTT CCCTGAAGGC TATGGGCATC AATGATTTAC TCTCCTTTGA CTTTATGGAT 
GCTCCCCCAA TGGAAACTCT CATCACTGCC ATGGAGCAGC TGTACACCCT GGGGGCTCTG 
GATGATGAGG GACTTCTCAC TCGACTTGGG CGCAGGATGG CAGAATTCCC CTTGGAGCCT 
ATGTTGTGTA AGATGTTGAT CATGTCTGTG CACCTGGGAT GCAGCGAGGA AATGCTGACA 
ATAGTCTCCA TGCTCTCTGT ACAGAATGTA TTCTACAGGC CAAAGGATAA ACAAGCACTT 
GCTGATCAGA AGAAAGCCAA GTTCCATCAG ACAGAAGGTG ACCACCTCAC TCTTCTGGCT 
GTCTACAATT CCTGGAAGAA TAATAAGTTT TCAAATCCCT GGTGCTATGA AAATTTCATT 
CAGGCTCGAT CCTTACGCAG GGCACAGGAC ATCCGCAAGC AGATGTTGGG CATTATGGAC 
AGGCACAAGC TGGATGTGGT ATCCTGTGGG AAGGCAACGG TTCGTGTCCA GAAGGCCATT 
TGTAGCGGCT TCTTCCGAAA CGCGGCAAAG AAGGATCCTC AGGAGGGTTA TCGGACACTC 
ATTGATCAAC AAGTGGTTTA TATCCACCCA TCCAGTGCTC TCTTCAACAG GCAGCCAGAG 
TGGGTGGTGT ATCACGAGCT GGTCCTGACC ACCAAGGAAT ACATGCGTGA GGTGACGACT 
ATCGATCCTC GCTGGCTGGT GGAATTTGCA CCAGCTTTCT TCAAGGTTTC TGATCCAACC 
AAGCTGAGCA AACAGAAGAA ACAGCAGCGT CTGGAACCCT TGTACAATCG CTACGAGGAG 
CCCAATGCTT GGAGGATATC TCGTGCCTTC AGACGACGAT GA