LOC778563 cDNA ORF clone, Ciona intestinalis(vase tunicate)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC778563
Entrez Gene ID	778563
Full Name	chordin
Gene Type	protein-coding
Organism	Ciona intestinalis(vase tunicate)

mRNA and Protein(s)

mRNA	Protein	Name
XM_026834784.1	XP_026690585.1	chordin
XM_002123270.4	XP_002123306.3	chordin

Pathways from BioSystems
Gene Ontology

The following LOC778563 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC778563 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCi13793	XM_002123270.4 Latest version!	Ciona intestinalis chordin (LOC778563), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCi156049	XM_026834784.1 Latest version!	Ciona intestinalis chordin (LOC778563), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC778563 ( XM_002123270.4 ) cDNA ORF clone, Ciona intestinalis(vase tunicate) -> XP_002123306.3 Ciona intestinalis chordin (LOC778563), mRNA.

Price & Availability↑

CloneID	OCi13793
Clone ID Related Accession (Same CDS sequence)	XM_002123270.4
Accession Version	XM_002123270.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2952bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-20
Organism	Ciona intestinalis(vase tunicate)
Product	chordin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_020171.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Oct 21, 2016 this sequence version replaced XM_002123270.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ciona intestinalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA CGTCATGCTA TTACGTCACG GTGGTATTCA TTGCTATGAC GTCAGGTATA 
TGTGCTGGCT TTCTGCAGAT ACATCCAGCA GAAGAAGCAT TGCCGTCTAA TAACCCGCGA 
GGTTGTGTAT TTGGTCGTCA GTTCCACGTC ATCGGATCAT CATGGCACCC AAACCTTGGT 
CGTCCATTTG GAATCATGTA TTGTGTCACG TGTCAGTGCG TCAAAGAGAC CACTGGGCGT 
TTCAAACCAG ACGGACAACC AAAGGAAGTT ACTCGGGTCA CGTGTCATGA CGTAAGAAAG 
GACTGCCCCC ACGTCACGTG CAAAGGGGCC AAGGTACCCA GGGGAGGATG CTGTAAAGTT 
TGCCCAGACG ATACAGATAC AATTGTGACG TCAGCCGAAG AATTTTCATC GAAAAATGGT 
GATGTTCCGC TGCCATTGGA TATCACTGAA GAAAAATCAA TTAACCACGA CGACTCAAAG 
GCTGGTGTTG ATGAGAAGAC AAAAAGTTAT GTTGCACTGT TAACCAGCAA TGATGGTGGG 
CCATTGTTAC GAGCTACGTT TAACCTGCAC AGGGATAATC TGCATTTTAC GATCCAGTAT 
TTGAGGTCAA CCAAACCCAC CATTATAGAA ATGCTGACTA ATGATGATGT CACAATATAC 
ACTCATGTAA CTGAAATGTC ACCAAAGCCA GGACAACCTA TTGTTTGCGG GGTATGGCGT 
AATCTACCAA AACACGTTGT TAGCTCCTTA GATAAAAGGT TGTTGACTCT CAGAGCTGTT 
TTTGTCCAAA CTGGTGCCAA TATGACATCA CAAGGTCAAA TAATACGACA CAGAGCATTG 
AGTCGAGAAA CTTTTAGTTC GATTTTATCC ACGACACCTC TAGATGGAGC TAGTGGAGCA 
ATTGCGATGA TCACCCTTGG CCGACATCGA TTCAATAAAC TTCATTTTGC CATTTTACTC 
AGACGATCTT CTGTGACATC ACAACCCACC ACAATCACTG TGCGATTACT CAACAATCAA 
AACAGAACGT TGCGATCAAC CGAGAAAACT CTTCAACCCT CAAGTAATGA GCTAGCTGAT 
GTGTGGTCGA CAAATTCTGA AATCCTTCGA AGATTGGGTG ATGGTTCACT TCATATACAG 
ATGGTTGCTA GGACAACAGG GTCACGTGAT AAACAAAGCT ACATCGGCAG CGTGAGAGCA 
AAAACAACTT GTGACCTGTT GCAAGCTGTT TTATCTGGAA GTGACTGTAG CCTACCAGCC 
AGTACAGGAG CAGCTGGATC CGCGATTCTG AATATTAATT CTGACAACAC TGTTTCTTAC 
AAGGTGGCGC TAGTGGGTAT TTCAAGTCGG GTGACCTCAA TATCACTGCT TGGAGACTAC 
AGAAAGAAGC GAACACGCGT AGTAGCCGAT CTCACCCCAC AATTTGTGAA TGGGAAAGCC 
CAAGGAAGAC TGGAAAACCT TAATGGGAAA GAGATACATC TTTTACTAAG CAACCGTGTG 
CGTGTTGCCG TGGAAACTGA ACAAAGCAAT AGTGGAGAGC TGGGTGGTCA TGTGACTTCT 
CTCCTTTATG GAGGCCACCA AGCTAGATAT AAAGGCCTTC CAATTCCTCT TGCTGGTTCT 
CTAGTACGAC CCCCTGTATT CACTGGGGCT GCAGGTCACG TTTGGCTTGA ACTGAATGAA 
GAATGTCATC TTTATTATGA AATTGTTGTT TCTGGACTAA GCAAAGAGCG TGACACAACA 
TTAGCCGCAC ATTTACACGG TCTCGCTGAA ATAGCTGAAA CAGAAACTGG ACATAAACAA 
CTTTTGAAAG GTTTCTATGG AAAAGAGGCG CGTGGAATAT TAAGAGAGTT ATCTTCAGAA 
ATATATGAAC ATCTCAACCA GGGCAATGCT TTTGTACAGA TTGCAACAAA GTCAAACCCA 
GGTGGAGAAA TACGTGGTCG TGTTCATATA CCAAACACTT GCTCCAGGAC AATAGTTAAC 
AAGGCTAACC ATAAACAAGA TACTTCAGAC ACAGACCAGA ATTCATTAAG TGTTCCAAGC 
TCTGATGTCA CCACGTCACG GGTGACTGAT CTCGATGCAG TGGTGCATGA CGGGGTAATC 
CCAACATACA CCGAGCGTCG TTTGGAGCTT GACCCATCCG CTTGTTACGT CATAGACGAA 
TGGAAACCCC ATGGTAGTGA ATGGGCACCA GATTACGACC CAAAATGCAC AATATGCGTT 
TGTGAGTCCG GTAGTTCACT TTGCGATCCT GTTTACTGCC CGCCACTAGA GTGCGGCAGA 
CCAGTGGCCA CCGAAGGGAG CTGTTGTCCA GTGTGTAACG ATGGGTTTGG GGAAATACCA 
ACCAAGCATG ATGATGGGGC TTCTATAGGA TCTCTGTTTG GGTCTCCATT TACACAACTC 
GATCAAAAAG AAGACGAGAA AGAAAAAGAG AAAGAAGGTT GCTACTACGG GGGCGATGGA 
CAAATACACG CGATAGGTTC ATGGTGGCAT CCATATTTTA AACGATTCGG CCACGTATCG 
TGCGTGAACT GCACGTGTAG GCCAGACGGT GAAATTGTGT GTGGAAAAAT TACGTGCCCG 
CCAGTAACGT GCTCAAATCC TATCAAACAA AATCCGAGAG ATTGCTGTAA AACTTGCCCA 
GAGGTCACAC GTCACGAACT ACGTCACGAA TCACGTGACC ATCGCATGCA GGACGACAGC 
GTGTCCAGGA TGTGTCAGTT TGGTCGTGAG CTACGAGCAC CCGGGGAAAA ATGGACGCTT 
GGATATCTGG GCAGAAGAAA CCTTGAATGT ATAGAATGCG AATGTGCTAC CCATGGTGTG 
AAACACAAGT GCCGCAAAAA CTGTCCAGCT GTCAGTCATG AATGCAGTCG AGTGGAACGA 
AACGTTTCCG GTTGCTGCCC ATGGCGTTGC GCGGACCGTC AGAGGTCGAC CGAGAGATTT 
CCGTTTTCGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002123306.3
CDS	76..3027
Translation	MMMTSCYYVTVVFIAMTSGICAGFLQIHPAEEALPSNNPRGCVFGRQFHVIGSSWHPNLGRPFGIMYCVTCQCVKETTGRFKPDGQPKEVTRVTCHDVRKDCPHVTCKGAKVPRGGCCKVCPDDTDTIVTSAEEFSSKNGDVPLPLDITEEKSINHDDSKAGVDEKTKSYVALLTSNDGGPLLRATFNLHRDNLHFTIQYLRSTKPTIIEMLTNDDVTIYTHVTEMSPKPGQPIVCGVWRNLPKHVVSSLDKRLLTLRAVFVQTGANMTSQGQIIRHRALSRETFSSILSTTPLDGASGAIAMITLGRHRFNKLHFAILLRRSSVTSQPTTITVRLLNNQNRTLRSTEKTLQPSSNELADVWSTNSEILRRLGDGSLHIQMVARTTGSRDKQSYIGSVRAKTTCDLLQAVLSGSDCSLPASTGAAGSAILNINSDNTVSYKVALVGISSRVTSISLLGDYRKKRTRVVADLTPQFVNGKAQGRLENLNGKEIHLLLSNRVRVAVETEQSNSGELGGHVTSLLYGGHQARYKGLPIPLAGSLVRPPVFTGAAGHVWLELNEECHLYYEIVVSGLSKERDTTLAAHLHGLAEIAETETGHKQLLKGFYGKEARGILRELSSEIYEHLNQGNAFVQIATKSNPGGEIRGRVHIPNTCSRTIVNKANHKQDTSDTDQNSLSVPSSDVTTSRVTDLDAVVHDGVIPTYTERRLELDPSACYVIDEWKPHGSEWAPDYDPKCTICVCESGSSLCDPVYCPPLECGRPVATEGSCCPVCNDGFGEIPTKHDDGASIGSLFGSPFTQLDQKEDEKEKEKEGCYYGGDGQIHAIGSWWHPYFKRFGHVSCVNCTCRPDGEIVCGKITCPPVTCSNPIKQNPRDCCKTCPEVTRHELRHESRDHRMQDDSVSRMCQFGRELRAPGEKWTLGYLGRRNLECIECECATHGVKHKCRKNCPAVSHECSRVERNVSGCCPWRCADRQRSTERFPFS

Target ORF information:

RefSeq Version	XM_002123270.4
Organism	Ciona intestinalis(vase tunicate)
Definition	Ciona intestinalis chordin (LOC778563), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002123270.4

ORF Insert Sequence:

ATGATGATGA CGTCATGCTA TTACGTCACG GTGGTATTCA TTGCTATGAC GTCAGGTATA 
TGTGCTGGCT TTCTGCAGAT ACATCCAGCA GAAGAAGCAT TGCCGTCTAA TAACCCGCGA 
GGTTGTGTAT TTGGTCGTCA GTTCCACGTC ATCGGATCAT CATGGCACCC AAACCTTGGT 
CGTCCATTTG GAATCATGTA TTGTGTCACG TGTCAGTGCG TCAAAGAGAC CACTGGGCGT 
TTCAAACCAG ACGGACAACC AAAGGAAGTT ACTCGGGTCA CGTGTCATGA CGTAAGAAAG 
GACTGCCCCC ACGTCACGTG CAAAGGGGCC AAGGTACCCA GGGGAGGATG CTGTAAAGTT 
TGCCCAGACG ATACAGATAC AATTGTGACG TCAGCCGAAG AATTTTCATC GAAAAATGGT 
GATGTTCCGC TGCCATTGGA TATCACTGAA GAAAAATCAA TTAACCACGA CGACTCAAAG 
GCTGGTGTTG ATGAGAAGAC AAAAAGTTAT GTTGCACTGT TAACCAGCAA TGATGGTGGG 
CCATTGTTAC GAGCTACGTT TAACCTGCAC AGGGATAATC TGCATTTTAC GATCCAGTAT 
TTGAGGTCAA CCAAACCCAC CATTATAGAA ATGCTGACTA ATGATGATGT CACAATATAC 
ACTCATGTAA CTGAAATGTC ACCAAAGCCA GGACAACCTA TTGTTTGCGG GGTATGGCGT 
AATCTACCAA AACACGTTGT TAGCTCCTTA GATAAAAGGT TGTTGACTCT CAGAGCTGTT 
TTTGTCCAAA CTGGTGCCAA TATGACATCA CAAGGTCAAA TAATACGACA CAGAGCATTG 
AGTCGAGAAA CTTTTAGTTC GATTTTATCC ACGACACCTC TAGATGGAGC TAGTGGAGCA 
ATTGCGATGA TCACCCTTGG CCGACATCGA TTCAATAAAC TTCATTTTGC CATTTTACTC 
AGACGATCTT CTGTGACATC ACAACCCACC ACAATCACTG TGCGATTACT CAACAATCAA 
AACAGAACGT TGCGATCAAC CGAGAAAACT CTTCAACCCT CAAGTAATGA GCTAGCTGAT 
GTGTGGTCGA CAAATTCTGA AATCCTTCGA AGATTGGGTG ATGGTTCACT TCATATACAG 
ATGGTTGCTA GGACAACAGG GTCACGTGAT AAACAAAGCT ACATCGGCAG CGTGAGAGCA 
AAAACAACTT GTGACCTGTT GCAAGCTGTT TTATCTGGAA GTGACTGTAG CCTACCAGCC 
AGTACAGGAG CAGCTGGATC CGCGATTCTG AATATTAATT CTGACAACAC TGTTTCTTAC 
AAGGTGGCGC TAGTGGGTAT TTCAAGTCGG GTGACCTCAA TATCACTGCT TGGAGACTAC 
AGAAAGAAGC GAACACGCGT AGTAGCCGAT CTCACCCCAC AATTTGTGAA TGGGAAAGCC 
CAAGGAAGAC TGGAAAACCT TAATGGGAAA GAGATACATC TTTTACTAAG CAACCGTGTG 
CGTGTTGCCG TGGAAACTGA ACAAAGCAAT AGTGGAGAGC TGGGTGGTCA TGTGACTTCT 
CTCCTTTATG GAGGCCACCA AGCTAGATAT AAAGGCCTTC CAATTCCTCT TGCTGGTTCT 
CTAGTACGAC CCCCTGTATT CACTGGGGCT GCAGGTCACG TTTGGCTTGA ACTGAATGAA 
GAATGTCATC TTTATTATGA AATTGTTGTT TCTGGACTAA GCAAAGAGCG TGACACAACA 
TTAGCCGCAC ATTTACACGG TCTCGCTGAA ATAGCTGAAA CAGAAACTGG ACATAAACAA 
CTTTTGAAAG GTTTCTATGG AAAAGAGGCG CGTGGAATAT TAAGAGAGTT ATCTTCAGAA 
ATATATGAAC ATCTCAACCA GGGCAATGCT TTTGTACAGA TTGCAACAAA GTCAAACCCA 
GGTGGAGAAA TACGTGGTCG TGTTCATATA CCAAACACTT GCTCCAGGAC AATAGTTAAC 
AAGGCTAACC ATAAACAAGA TACTTCAGAC ACAGACCAGA ATTCATTAAG TGTTCCAAGC 
TCTGATGTCA CCACGTCACG GGTGACTGAT CTCGATGCAG TGGTGCATGA CGGGGTAATC 
CCAACATACA CCGAGCGTCG TTTGGAGCTT GACCCATCCG CTTGTTACGT CATAGACGAA 
TGGAAACCCC ATGGTAGTGA ATGGGCACCA GATTACGACC CAAAATGCAC AATATGCGTT 
TGTGAGTCCG GTAGTTCACT TTGCGATCCT GTTTACTGCC CGCCACTAGA GTGCGGCAGA 
CCAGTGGCCA CCGAAGGGAG CTGTTGTCCA GTGTGTAACG ATGGGTTTGG GGAAATACCA 
ACCAAGCATG ATGATGGGGC TTCTATAGGA TCTCTGTTTG GGTCTCCATT TACACAACTC 
GATCAAAAAG AAGACGAGAA AGAAAAAGAG AAAGAAGGTT GCTACTACGG GGGCGATGGA 
CAAATACACG CGATAGGTTC ATGGTGGCAT CCATATTTTA AACGATTCGG CCACGTATCG 
TGCGTGAACT GCACGTGTAG GCCAGACGGT GAAATTGTGT GTGGAAAAAT TACGTGCCCG 
CCAGTAACGT GCTCAAATCC TATCAAACAA AATCCGAGAG ATTGCTGTAA AACTTGCCCA 
GAGGTCACAC GTCACGAACT ACGTCACGAA TCACGTGACC ATCGCATGCA GGACGACAGC 
GTGTCCAGGA TGTGTCAGTT TGGTCGTGAG CTACGAGCAC CCGGGGAAAA ATGGACGCTT 
GGATATCTGG GCAGAAGAAA CCTTGAATGT ATAGAATGCG AATGTGCTAC CCATGGTGTG 
AAACACAAGT GCCGCAAAAA CTGTCCAGCT GTCAGTCATG AATGCAGTCG AGTGGAACGA 
AACGTTTCCG GTTGCTGCCC ATGGCGTTGC GCGGACCGTC AGAGGTCGAC CGAGAGATTT 
CCGTTTTCGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC778563 ( XM_026834784.1 ) cDNA ORF clone, Ciona intestinalis(vase tunicate) -> XP_026690585.1 Ciona intestinalis chordin (LOC778563), mRNA.

Price & Availability↑

CloneID	OCi156049
Clone ID Related Accession (Same CDS sequence)	XM_026834784.1
Accession Version	XM_026834784.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3057bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-21
Organism	Ciona intestinalis(vase tunicate)
Product	chordin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_020171.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ciona intestinalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCGTG ACGTCACGGG AAAAAGGCTA CGTCACAGTG GGGGTACTTT ATCTAGGGAT 
CTCGTAGATT TCACCATATC GAAATCTCAC TTCGAGCAGG AACAAATGAT GATGACGTCA 
TGCTATTACG TCACGGTGGT ATTCATTGCT ATGACGTCAG GTATATGTGC TGGCTTTCTG 
CAGATACATC CAGCAGAAGA AGCATTGCCG TCTAATAACC CGCGAGGTTG TGTATTTGGT 
CGTCAGTTCC ACGTCATCGG ATCATCATGG CACCCAAACC TTGGTCGTCC ATTTGGAATC 
ATGTATTGTG TCACGTGTCA GTGCGTCAAA GAGACCACTG GGCGTTTCAA ACCAGACGGA 
CAACCAAAGG AAGTTACTCG GGTCACGTGT CATGACGTAA GAAAGGACTG CCCCCACGTC 
ACGTGCAAAG GGGCCAAGGT ACCCAGGGGA GGATGCTGTA AAGTTTGCCC AGACGATACA 
GATACAATTG TGACGTCAGC CGAAGAATTT TCATCGAAAA ATGGTGATGT TCCGCTGCCA 
TTGGATATCA CTGAAGAAAA ATCAATTAAC CACGACGACT CAAAGGCTGG TGTTGATGAG 
AAGACAAAAA GTTATGTTGC ACTGTTAACC AGCAATGATG GTGGGCCATT GTTACGAGCT 
ACGTTTAACC TGCACAGGGA TAATCTGCAT TTTACGATCC AGTATTTGAG GTCAACCAAA 
CCCACCATTA TAGAAATGCT GACTAATGAT GATGTCACAA TATACACTCA TGTAACTGAA 
ATGTCACCAA AGCCAGGACA ACCTATTGTT TGCGGGGTAT GGCGTAATCT ACCAAAACAC 
GTTGTTAGCT CCTTAGATAA AAGGTTGTTG ACTCTCAGAG CTGTTTTTGT CCAAACTGGT 
GCCAATATGA CATCACAAGG TCAAATAATA CGACACAGAG CATTGAGTCG AGAAACTTTT 
AGTTCGATTT TATCCACGAC ACCTCTAGAT GGAGCTAGTG GAGCAATTGC GATGATCACC 
CTTGGCCGAC ATCGATTCAA TAAACTTCAT TTTGCCATTT TACTCAGACG ATCTTCTGTG 
ACATCACAAC CCACCACAAT CACTGTGCGA TTACTCAACA ATCAAAACAG AACGTTGCGA 
TCAACCGAGA AAACTCTTCA ACCCTCAAGT AATGAGCTAG CTGATGTGTG GTCGACAAAT 
TCTGAAATCC TTCGAAGATT GGGTGATGGT TCACTTCATA TACAGATGGT TGCTAGGACA 
ACAGGGTCAC GTGATAAACA AAGCTACATC GGCAGCGTGA GAGCAAAAAC AACTTGTGAC 
CTGTTGCAAG CTGTTTTATC TGGAAGTGAC TGTAGCCTAC CAGCCAGTAC AGGAGCAGCT 
GGATCCGCGA TTCTGAATAT TAATTCTGAC AACACTGTTT CTTACAAGGT GGCGCTAGTG 
GGTATTTCAA GTCGGGTGAC CTCAATATCA CTGCTTGGAG ACTACAGAAA GAAGCGAACA 
CGCGTAGTAG CCGATCTCAC CCCACAATTT GTGAATGGGA AAGCCCAAGG AAGACTGGAA 
AACCTTAATG GGAAAGAGAT ACATCTTTTA CTAAGCAACC GTGTGCGTGT TGCCGTGGAA 
ACTGAACAAA GCAATAGTGG AGAGCTGGGT GGTCATGTGA CTTCTCTCCT TTATGGAGGC 
CACCAAGCTA GATATAAAGG CCTTCCAATT CCTCTTGCTG GTTCTCTAGT ACGACCCCCT 
GTATTCACTG GGGCTGCAGG TCACGTTTGG CTTGAACTGA ATGAAGAATG TCATCTTTAT 
TATGAAATTG TTGTTTCTGG ACTAAGCAAA GAGCGTGACA CAACATTAGC CGCACATTTA 
CACGGTCTCG CTGAAATAGC TGAAACAGAA ACTGGACATA AACAACTTTT GAAAGGTTTC 
TATGGAAAAG AGGCGCGTGG AATATTAAGA GAGTTATCTT CAGAAATATA TGAACATCTC 
AACCAGGGCA ATGCTTTTGT ACAGATTGCA ACAAAGTCAA ACCCAGGTGG AGAAATACGT 
GGTCGTGTTC ATATACCAAA CACTTGCTCC AGGACAATAG TTAACAAGGC TAACCATAAA 
CAAGATACTT CAGACACAGA CCAGAATTCA TTAAGTGTTC CAAGCTCTGA TGTCACCACG 
TCACGGGTGA CTGATCTCGA TGCAGTGGTG CATGACGGGG TAATCCCAAC ATACACCGAG 
CGTCGTTTGG AGCTTGACCC ATCCGCTTGT TACGTCATAG ACGAATGGAA ACCCCATGGT 
AGTGAATGGG CACCAGATTA CGACCCAAAA TGCACAATAT GCGTTTGTGA GTCCGGTAGT 
TCACTTTGCG ATCCTGTTTA CTGCCCGCCA CTAGAGTGCG GCAGACCAGT GGCCACCGAA 
GGGAGCTGTT GTCCAGTGTG TAACGATGGG TTTGGGGAAA TACCAACCAA GCATGATGAT 
GGGGCTTCTA TAGGATCTCT GTTTGGGTCT CCATTTACAC AACTCGATCA AAAAGAAGAC 
GAGAAAGAAA AAGAGAAAGA AGGTTGCTAC TACGGGGGCG ATGGACAAAT ACACGCGATA 
GGTTCATGGT GGCATCCATA TTTTAAACGA TTCGGCCACG TATCGTGCGT GAACTGCACG 
TGTAGGCCAG ACGGTGAAAT TGTGTGTGGA AAAATTACGT GCCCGCCAGT AACGTGCTCA 
AATCCTATCA AACAAAATCC GAGAGATTGC TGTAAAACTT GCCCAGAGGT CACACGTCAC 
GAACTACGTC ACGAATCACG TGACCATCGC ATGCAGGACG ACAGCGTGTC CAGGATGTGT 
CAGTTTGGTC GTGAGCTACG AGCACCCGGG GAAAAATGGA CGCTTGGATA TCTGGGCAGA 
AGAAACCTTG AATGTATAGA ATGCGAATGT GCTACCCATG GTGTGAAACA CAAGTGCCGC 
AAAAACTGTC CAGCTGTCAG TCATGAATGC AGTCGAGTGG AACGAAACGT TTCCGGTTGC 
TGCCCATGGC GTTGCGCGGA CCGTCAGAGG TCGACCGAGA GATTTCCGTT TTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026690585.1
CDS	16..3072
Translation	MNRDVTGKRLRHSGGTLSRDLVDFTISKSHFEQEQMMMTSCYYVTVVFIAMTSGICAGFLQIHPAEEALPSNNPRGCVFGRQFHVIGSSWHPNLGRPFGIMYCVTCQCVKETTGRFKPDGQPKEVTRVTCHDVRKDCPHVTCKGAKVPRGGCCKVCPDDTDTIVTSAEEFSSKNGDVPLPLDITEEKSINHDDSKAGVDEKTKSYVALLTSNDGGPLLRATFNLHRDNLHFTIQYLRSTKPTIIEMLTNDDVTIYTHVTEMSPKPGQPIVCGVWRNLPKHVVSSLDKRLLTLRAVFVQTGANMTSQGQIIRHRALSRETFSSILSTTPLDGASGAIAMITLGRHRFNKLHFAILLRRSSVTSQPTTITVRLLNNQNRTLRSTEKTLQPSSNELADVWSTNSEILRRLGDGSLHIQMVARTTGSRDKQSYIGSVRAKTTCDLLQAVLSGSDCSLPASTGAAGSAILNINSDNTVSYKVALVGISSRVTSISLLGDYRKKRTRVVADLTPQFVNGKAQGRLENLNGKEIHLLLSNRVRVAVETEQSNSGELGGHVTSLLYGGHQARYKGLPIPLAGSLVRPPVFTGAAGHVWLELNEECHLYYEIVVSGLSKERDTTLAAHLHGLAEIAETETGHKQLLKGFYGKEARGILRELSSEIYEHLNQGNAFVQIATKSNPGGEIRGRVHIPNTCSRTIVNKANHKQDTSDTDQNSLSVPSSDVTTSRVTDLDAVVHDGVIPTYTERRLELDPSACYVIDEWKPHGSEWAPDYDPKCTICVCESGSSLCDPVYCPPLECGRPVATEGSCCPVCNDGFGEIPTKHDDGASIGSLFGSPFTQLDQKEDEKEKEKEGCYYGGDGQIHAIGSWWHPYFKRFGHVSCVNCTCRPDGEIVCGKITCPPVTCSNPIKQNPRDCCKTCPEVTRHELRHESRDHRMQDDSVSRMCQFGRELRAPGEKWTLGYLGRRNLECIECECATHGVKHKCRKNCPAVSHECSRVERNVSGCCPWRCADRQRSTERFPFS

Target ORF information:

RefSeq Version	XM_026834784.1
Organism	Ciona intestinalis(vase tunicate)
Definition	Ciona intestinalis chordin (LOC778563), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026834784.1

ORF Insert Sequence:

ATGAACCGTG ACGTCACGGG AAAAAGGCTA CGTCACAGTG GGGGTACTTT ATCTAGGGAT 
CTCGTAGATT TCACCATATC GAAATCTCAC TTCGAGCAGG AACAAATGAT GATGACGTCA 
TGCTATTACG TCACGGTGGT ATTCATTGCT ATGACGTCAG GTATATGTGC TGGCTTTCTG 
CAGATACATC CAGCAGAAGA AGCATTGCCG TCTAATAACC CGCGAGGTTG TGTATTTGGT 
CGTCAGTTCC ACGTCATCGG ATCATCATGG CACCCAAACC TTGGTCGTCC ATTTGGAATC 
ATGTATTGTG TCACGTGTCA GTGCGTCAAA GAGACCACTG GGCGTTTCAA ACCAGACGGA 
CAACCAAAGG AAGTTACTCG GGTCACGTGT CATGACGTAA GAAAGGACTG CCCCCACGTC 
ACGTGCAAAG GGGCCAAGGT ACCCAGGGGA GGATGCTGTA AAGTTTGCCC AGACGATACA 
GATACAATTG TGACGTCAGC CGAAGAATTT TCATCGAAAA ATGGTGATGT TCCGCTGCCA 
TTGGATATCA CTGAAGAAAA ATCAATTAAC CACGACGACT CAAAGGCTGG TGTTGATGAG 
AAGACAAAAA GTTATGTTGC ACTGTTAACC AGCAATGATG GTGGGCCATT GTTACGAGCT 
ACGTTTAACC TGCACAGGGA TAATCTGCAT TTTACGATCC AGTATTTGAG GTCAACCAAA 
CCCACCATTA TAGAAATGCT GACTAATGAT GATGTCACAA TATACACTCA TGTAACTGAA 
ATGTCACCAA AGCCAGGACA ACCTATTGTT TGCGGGGTAT GGCGTAATCT ACCAAAACAC 
GTTGTTAGCT CCTTAGATAA AAGGTTGTTG ACTCTCAGAG CTGTTTTTGT CCAAACTGGT 
GCCAATATGA CATCACAAGG TCAAATAATA CGACACAGAG CATTGAGTCG AGAAACTTTT 
AGTTCGATTT TATCCACGAC ACCTCTAGAT GGAGCTAGTG GAGCAATTGC GATGATCACC 
CTTGGCCGAC ATCGATTCAA TAAACTTCAT TTTGCCATTT TACTCAGACG ATCTTCTGTG 
ACATCACAAC CCACCACAAT CACTGTGCGA TTACTCAACA ATCAAAACAG AACGTTGCGA 
TCAACCGAGA AAACTCTTCA ACCCTCAAGT AATGAGCTAG CTGATGTGTG GTCGACAAAT 
TCTGAAATCC TTCGAAGATT GGGTGATGGT TCACTTCATA TACAGATGGT TGCTAGGACA 
ACAGGGTCAC GTGATAAACA AAGCTACATC GGCAGCGTGA GAGCAAAAAC AACTTGTGAC 
CTGTTGCAAG CTGTTTTATC TGGAAGTGAC TGTAGCCTAC CAGCCAGTAC AGGAGCAGCT 
GGATCCGCGA TTCTGAATAT TAATTCTGAC AACACTGTTT CTTACAAGGT GGCGCTAGTG 
GGTATTTCAA GTCGGGTGAC CTCAATATCA CTGCTTGGAG ACTACAGAAA GAAGCGAACA 
CGCGTAGTAG CCGATCTCAC CCCACAATTT GTGAATGGGA AAGCCCAAGG AAGACTGGAA 
AACCTTAATG GGAAAGAGAT ACATCTTTTA CTAAGCAACC GTGTGCGTGT TGCCGTGGAA 
ACTGAACAAA GCAATAGTGG AGAGCTGGGT GGTCATGTGA CTTCTCTCCT TTATGGAGGC 
CACCAAGCTA GATATAAAGG CCTTCCAATT CCTCTTGCTG GTTCTCTAGT ACGACCCCCT 
GTATTCACTG GGGCTGCAGG TCACGTTTGG CTTGAACTGA ATGAAGAATG TCATCTTTAT 
TATGAAATTG TTGTTTCTGG ACTAAGCAAA GAGCGTGACA CAACATTAGC CGCACATTTA 
CACGGTCTCG CTGAAATAGC TGAAACAGAA ACTGGACATA AACAACTTTT GAAAGGTTTC 
TATGGAAAAG AGGCGCGTGG AATATTAAGA GAGTTATCTT CAGAAATATA TGAACATCTC 
AACCAGGGCA ATGCTTTTGT ACAGATTGCA ACAAAGTCAA ACCCAGGTGG AGAAATACGT 
GGTCGTGTTC ATATACCAAA CACTTGCTCC AGGACAATAG TTAACAAGGC TAACCATAAA 
CAAGATACTT CAGACACAGA CCAGAATTCA TTAAGTGTTC CAAGCTCTGA TGTCACCACG 
TCACGGGTGA CTGATCTCGA TGCAGTGGTG CATGACGGGG TAATCCCAAC ATACACCGAG 
CGTCGTTTGG AGCTTGACCC ATCCGCTTGT TACGTCATAG ACGAATGGAA ACCCCATGGT 
AGTGAATGGG CACCAGATTA CGACCCAAAA TGCACAATAT GCGTTTGTGA GTCCGGTAGT 
TCACTTTGCG ATCCTGTTTA CTGCCCGCCA CTAGAGTGCG GCAGACCAGT GGCCACCGAA 
GGGAGCTGTT GTCCAGTGTG TAACGATGGG TTTGGGGAAA TACCAACCAA GCATGATGAT 
GGGGCTTCTA TAGGATCTCT GTTTGGGTCT CCATTTACAC AACTCGATCA AAAAGAAGAC 
GAGAAAGAAA AAGAGAAAGA AGGTTGCTAC TACGGGGGCG ATGGACAAAT ACACGCGATA 
GGTTCATGGT GGCATCCATA TTTTAAACGA TTCGGCCACG TATCGTGCGT GAACTGCACG 
TGTAGGCCAG ACGGTGAAAT TGTGTGTGGA AAAATTACGT GCCCGCCAGT AACGTGCTCA 
AATCCTATCA AACAAAATCC GAGAGATTGC TGTAAAACTT GCCCAGAGGT CACACGTCAC 
GAACTACGTC ACGAATCACG TGACCATCGC ATGCAGGACG ACAGCGTGTC CAGGATGTGT 
CAGTTTGGTC GTGAGCTACG AGCACCCGGG GAAAAATGGA CGCTTGGATA TCTGGGCAGA 
AGAAACCTTG AATGTATAGA ATGCGAATGT GCTACCCATG GTGTGAAACA CAAGTGCCGC 
AAAAACTGTC CAGCTGTCAG TCATGAATGC AGTCGAGTGG AACGAAACGT TTCCGGTTGC 
TGCCCATGGC GTTGCGCGGA CCGTCAGAGG TCGACCGAGA GATTTCCGTT TTCGTAA