LOC100187226 gene cDNA ORF clone, Ciona intestinalis(vase tunicate)

mRNA	Protein	Name
XM_018813629.2	XP_018669174.1	eIF-2-alpha kinase GCN2 isoform X1
XM_018813630.1	XP_018669175.1	eIF-2-alpha kinase GCN2 isoform X2
XM_018813629.1	XP_018669174.1	eIF-2-alpha kinase GCN2 isoform X1
XM_026836280.1	XP_026692081.1	eIF-2-alpha kinase GCN2 isoform X4
XM_018813631.1	XP_018669176.1	eIF-2-alpha kinase GCN2 isoform X3
XM_018813632.1	XP_018669177.1	eIF-2-alpha kinase GCN2 isoform X4
XM_018813631.2	XP_018669176.1	eIF-2-alpha kinase GCN2 isoform X2
XM_026836279.1	XP_026692080.1	eIF-2-alpha kinase GCN2 isoform X3
XM_018813632.2	XP_018669177.1	eIF-2-alpha kinase GCN2 isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCi153692	XM_026836279.1 Latest version!	Ciona intestinalis eIF-2-alpha kinase GCN2 (LOC100187226), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OCi153693	XM_026836280.1 Latest version!	Ciona intestinalis eIF-2-alpha kinase GCN2 (LOC100187226), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OCi70880	XM_018813629.2 Latest version!	Ciona intestinalis eIF-2-alpha kinase GCN2 (LOC100187226), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCi70881	XM_018813630.1 Latest version!	Ciona intestinalis eIF-2-alpha kinase GCN2 (LOC100187226), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCi70882	XM_018813631.2 Latest version!	Ciona intestinalis eIF-2-alpha kinase GCN2 (LOC100187226), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCi70883	XM_018813632.2 Latest version!	Ciona intestinalis eIF-2-alpha kinase GCN2 (LOC100187226), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
View Old Accession Versions >>
OCi70880	XM_018813629.1	Ciona intestinalis eIF-2-alpha kinase GCN2 (LOC100187226), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCi70882	XM_018813631.1	Ciona intestinalis eIF-2-alpha kinase GCN2 (LOC100187226), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCi70883	XM_018813632.1	Ciona intestinalis eIF-2-alpha kinase GCN2 (LOC100187226), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
Hide Old Accession Versions >>

LOC100187226 ( XM_026836279.1 ) cDNA ORF clone, Ciona intestinalis(vase tunicate) -> XP_026692080.1 Ciona intestinalis eIF-2-alpha kinase GCN2 (LOC100187226), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCi153692
Clone ID Related Accession (Same CDS sequence)	XM_026836279.1
Accession Version	XM_026836279.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-21
Organism	Ciona intestinalis(vase tunicate)
Product	eIF-2-alpha kinase GCN2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_020175.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ciona intestinalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGATGA CGGAAAGTGA ATCCTATCAA GAAAGGCAGG AAAATGAACT TGAATTGCTT 
CACTCCATAT TTGGTGAGGA GATTGAAGAT CTGAGGAAAT TGGACAAATG GAAGGTGGTC 
CGTCCTCCAG AGATCATTCT CTATCTTCAT CCACAGGAAG GGATGAGCGG AAAGAAAGAA 
ACTTTTACCA AAATTGACCT CAAAATAAAA TTTACACCAA CTTACCCAGA CAGTTTCCCC 
GAGTGTAAAC TGGAGAATGC GAAAGGTTTA TCAGAAGATG CCATAAATGA TTTGGAATCT 
GGGATTAAAA ATCTCTACAA GTCATTACAG GGTGAAGTAA TGGTGCTCGA AATTGCTCAG 
TTTGTTAAGG AGTTCCTGCA TACACACAAT GTCCCACAAT GTAACTCGGT GTACGAGGAA 
ATGGTAAAAA ACAAACAAAG ACAGCTTGAA AAAGCTGCAC AGGAAGAGGA AAAGAAAAAG 
GAAATTGAAA GAAGAAAAAA GGAATTAAAG AGGAAAGAGA TAGAAGAAGA ACTAGCAAGG 
AGGCAAAACG AAGAAAAACA AATTCGTAAA CTTATGAGGG AGGGCAGAGA TCAGAACCAA 
TCATCTCAAG ACCACACACG CCGACTTTCT GAAGATATTC CGAACGGCAA TGGCAGTCAT 
GGGAAGTCTA CAGGAGTCGT GGATTTAAAA ATCAATGGAA AACTTCCACG AATAATACAT 
AAAGGGCAAA CATTAGACTA CATTGGAAGA TCCACACAAA AACTTAAAAA GATCCAGAAG 
CATATCGAGA TCTGCCATTT AAACACCACC AGTAGTGGTA GCAATGGATC AACAGTATAC 
GCGGGCATGG ATGCAAACAC AGGTGAACTA GTTGCAGTTC ATTACTGGAA CATACCATGT 
CCTATGAGGA ATTCAATGCT CACTGACGCA GAACGAGCTA AACTCGATAA ATATATGAAA 
ACAATTAACA CAATTGAGCA GGAGTTGAAT TCTTTGGTGA AATTCTCCCA CAAACACCTA 
GTGCCCTACC TTGCAATGAG ATATAGCCAT GTTGAAAGTA GTATTGAACT TCATATTGTG 
GAGAAATATA TAACAGGTGC AAGCCTTCAA CAAAAACTCA CAAGTAGAGA CAGAGTTGGA 
ATTGACGCTG TTAGAACTTA TTCTGAGCAA CTTTTATCTG CGCTTGACTG CCTACATAAA 
AAGTCTGTTG TACATAAGAA TCTTAAGGCG AGCAGTGTTT TCTTAAGCAC CAAGGGATCC 
ATCAAGCTTG CAGATTACAG CATAGAGAAA AGAGTAATAG ACTTACATAA TGAGTCCCGT 
ATTGAACAGC CTGGTGTTCG ATTCAGTGAA GATCGAATTC CTGCTCGAAA TACAAAGAAA 
GGAGATATTT TGCGGTTGGG TATGTTGATA CTGTCACTTG CACAAGGACA AACAATACAG 
GATTATCCTC CAGAAATACC AGCGAGCTTT CCACTGGTTT TCCAAAACTT TCTTGAGAAA 
TGTCTTGTGG TTGAAGACAG GCTTCGTTAT ACAACATCTG AACTCTTGGA TCATGAATTT 
ATCAAGCCAA GTGTCACCAG TCCTATCCTT GACATCGATC TTCCTTTGGA GCAAAATCCG 
AGTCCGTTTG ATGAAGATAA GATGGATGAT GTCCTTGCAA CATCCGATGC TCCAAGACCA 
ACTCTCTCTC GTCTCCACTC GGAGTTTGAG AAGTTAGAAT GGCTTGGGAA GGGAGGGTAT 
GGGGACGTGG TGAAGGTAAA GAACATAATA GACGGACGAG ATTACGCAAT CAAACGTATT 
CTACTGAACC CACATCGATC CCTGTTTAAT AAAAAGATAA CGAGAGAAGT GAAACTTTTG 
TCTCGGTTAA ACCATGAGAA TGTTGTCAGA TATTACAACT CGTGGATTGA AACTGTTGAA 
TGTTGGGAAG ATGACGTTTC AACTAATTCA TCTAACCCAA CAACAACAAC AACAACAAAT 
GAAACAAAAT CAAACGTATC AGATCGTATC AATTCACTTG GTTTTTATGA TAATATTGAG 
GTTCTAGCGC CACCTCCTGT CGCATCTAGC GTGTCAGATT GGAGCGTTTC ATTGGAGGAG 
GTGAGTCGTT GTCGTGACGA CGAGGAAGAT GATGACGAAG ATGTGTTCAA GACTATCTAT 
GTTGACGAGG ATGACTCCGA GAGCATCAAG TTTGATCACA GTGATGAAGA AGAAGATGAG 
ATGGTGGAAG AATCAACTAA TGATGGCAAA ACGTCTCGGA GTGAAGAAAC CACCGTCACT 
AGTTCCGCGT CATCAAAATC GCTTGTGACT CAGCGATTTC TATACATACA GATGGAATAT 
TGTGAGAAGA GCACACTGCG TCAAACCATT GATGCTGGCG TTTGTAATGA ACCTGAGAGA 
ATGTGGAGAT TATTCAGGGA AATTCTTGAA GGATTGGTTC ACGTACATGA GCAAGGCATG 
ATCCATAGAG ATCTAAAACC ACAGAATATT TTCCTCGATA AAAACGACCA TGTTAAGATT 
GGTGATTTTG GTCTTGCTAC TGCACAAGCT AGAGTGACAC TAGCTGACAG CTCTGAAGAG 
ATTTCAAAAT CCGAAACTGG AGACAGTGTA TATTCTGGTG TGGTGGGAAC TGCATTATAC 
GTGGCACCTG AACTTATGGT TACTGGTAAA AGATCACTGT ACAGTCAAAA GGTTGACATC 
TACAGTGTTG GAATCATTTT CTTTGAAATG TCATTTCCTG GTATCCAAAC TAAGATGGAA 
CGAGTCAAAG TTCTTGGGAA CCTTAGAACT TCTTCCATAA CCTTCCCATC TGACTTTGAT 
GAAGCTGCCA TGCCTCGTGA AGCTGAGATA ACTCGATGGT GTTTGGATCA CGTGCCAAAC 
AAACGACCAA CTGCATCTGA ACTTCTAAAG TGTAGATTAG TTCCAACTCC TGTACTAGAA 
GAATCCAATG TACTTGAAGC AGTTCGTGAA ATGTTAAGCA ATCCAGACTC AAAGCCGTAT 
AGATTAGTTA TGGATGAAGT TTTCAACCAA CCAGAAAACC CAACTTCATC TGTTATATAC 
GACATTGAAA TGCATAAGAA ACATTTTTCT GAGACACAGA ATGTCGCACA ACAGCAAACA 
TTTGACATTA TATCTTCCCT ACTACGTTGC CATGGAGCTG TGCGGGTCAA TCCTCCGCTC 
TTGTTACCTA AATGTGGTTC AGAGAATGAT GCAAATGAAG CTGCAGTGCT AATAGGGGTT 
GGCGGTAATG TCGTATCACT GCCTTATATT CTAAGGATTC CACTGGCTCG TTTTATTCTG 
TGTAAAGGAA TCACAAACAT TAAAACTTAC GCCATTGAAA AAGTTTTCCG AGCAAGAAAA 
TATGCGAGGT CCCGACCTAA GGAATTATGG GAATGTTCGT TTGACATCAT TTCCCCCTCA 
GTGGGAGGTC TCGCTCCCGA CGCGGAAGTG ATCTCTGTTG TTTCCGAGAT CATCTCCCAA 
TTCCCATCTC TCTCCTCCCA GAAGTACGAG GTTCGGATCA ACCACTTATT GGCGCTAGAG 
TCAATACTAA CATACTGTGG TCTCGATGAA AGCCACCACA CGGAGGCGCT CAATGCACTC 
GCTTCATGCA GTGGCCTCTC TAAAACACAG CTCCACAACT GCATCAACAG CTTGCCTCTC 
AATGCTCAAC AGGTTGCTTC CCTTCTTACA TTCATCCAAG TCCAAGGCCC GCTGTCCCGG 
GTTCAAACCT CGCTGCGGTC GATTACCCGG AACAAGCACT CACCCGCGTC GTCCAGAGCA 
CGACAGGCGC TCCACGAACT CGAAGCTCTT CGGAAAAATC TTGATTCTTT AAACTTGACA 
CTACCTGTTG TGTTTGATCT TGGTTATTCT CATAAACACC ACAACCATTA TAATGGCATC 
ATATTTAAAG TTTCAGCGGA AATCCAAAAG AAAAAAAGGA CAGCAATTGA TGTGTTGGCC 
ATCGGTGGTC GATATGATAA ACTGCTCACA ACCATGTCTG TATTCAACAT GTATGACAGA 
GATGGGGAAC AGAAGTTAAC ACCTAAGCAT GCTGTGGGTG TTAGTATTGC TGTGGAGAAA 
ATTGTAGGAT CCGTAATGGA AGAAAACAAT CTTCCCAGCT CAACAAGTAT GAGTGGCAGC 
AACGACCCCA CTGTGACATC ACAATTATCC CACCACGCTG CAGTTGACGT GTTGGTTTGT 
TCTGTCGGGC GAACTCTTCT GTTGCACGAA CGATTGAGGG TTGTAAGATC TCTATGGGAG 
GCCTCGATAG CAGCAGAAAC CGTTTATGAT CGCGGAGCAA TGGAATCAAT GCAAGAAGTG 
CAGGAGAAAG CTCAAGAAAG GGGGATCCGC CATCTTGTAT TTTTAAGCGA GAAAGAAAGT 
GGCTCCCCGT TAGTACGATC ATTGACGCAC GACCCGCTGT TACGCTCTAC TGAACGCAGA 
GTACCGCTGG ATCAGTTGGT GGATTATTTG TTAAAACGAT TGAAAGAAGT TGAATCAACT 
GTATCAATGA AATCTTCCTC ATCGGGGTTC TCTGCATCAC ACGAGTCCTC CACTGTTACC 
ATGACGAACA ATAGTGCTCG TCAAATCAGC ATCGAGGTTG TTCCTCCCGA GAAACTTGCC 
TCACATTTAA GGAAGAGATA TGAGTCTGAA GTTATGACCA AACTTACAAG TCTACCTATG 
ATGTCCCACA TTCAATCTAA AGCTCAAATT TGTGCCCTTG CGGTTGAACT GCCATCACCA 
GTTATGAAGA GCATGGGATG TGCTCTTGAT CTTTCTCACT CTGAGGAAGA GTTTAACTCT 
TCTCTTGCCC ATCATCTACA CACTGTATCC AAGTATAAAA AATACTTGTC CTATATCGCT 
GAGAGAATTT GCGAGGCAAA ATTCGAACGA GAAATGACTT TCATCATTCT TTACAGCTAC 
AAAGACCATG GCTACTGTCT TTTGATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026692080.1
CDS	107..5056
Translation	MTMTESESYQERQENELELLHSIFGEEIEDLRKLDKWKVVRPPEIILYLHPQEGMSGKKETFTKIDLKIKFTPTYPDSFPECKLENAKGLSEDAINDLESGIKNLYKSLQGEVMVLEIAQFVKEFLHTHNVPQCNSVYEEMVKNKQRQLEKAAQEEEKKKEIERRKKELKRKEIEEELARRQNEEKQIRKLMREGRDQNQSSQDHTRRLSEDIPNGNGSHGKSTGVVDLKINGKLPRIIHKGQTLDYIGRSTQKLKKIQKHIEICHLNTTSSGSNGSTVYAGMDANTGELVAVHYWNIPCPMRNSMLTDAERAKLDKYMKTINTIEQELNSLVKFSHKHLVPYLAMRYSHVESSIELHIVEKYITGASLQQKLTSRDRVGIDAVRTYSEQLLSALDCLHKKSVVHKNLKASSVFLSTKGSIKLADYSIEKRVIDLHNESRIEQPGVRFSEDRIPARNTKKGDILRLGMLILSLAQGQTIQDYPPEIPASFPLVFQNFLEKCLVVEDRLRYTTSELLDHEFIKPSVTSPILDIDLPLEQNPSPFDEDKMDDVLATSDAPRPTLSRLHSEFEKLEWLGKGGYGDVVKVKNIIDGRDYAIKRILLNPHRSLFNKKITREVKLLSRLNHENVVRYYNSWIETVECWEDDVSTNSSNPTTTTTTNETKSNVSDRINSLGFYDNIEVLAPPPVASSVSDWSVSLEEVSRCRDDEEDDDEDVFKTIYVDEDDSESIKFDHSDEEEDEMVEESTNDGKTSRSEETTVTSSASSKSLVTQRFLYIQMEYCEKSTLRQTIDAGVCNEPERMWRLFREILEGLVHVHEQGMIHRDLKPQNIFLDKNDHVKIGDFGLATAQARVTLADSSEEISKSETGDSVYSGVVGTALYVAPELMVTGKRSLYSQKVDIYSVGIIFFEMSFPGIQTKMERVKVLGNLRTSSITFPSDFDEAAMPREAEITRWCLDHVPNKRPTASELLKCRLVPTPVLEESNVLEAVREMLSNPDSKPYRLVMDEVFNQPENPTSSVIYDIEMHKKHFSETQNVAQQQTFDIISSLLRCHGAVRVNPPLLLPKCGSENDANEAAVLIGVGGNVVSLPYILRIPLARFILCKGITNIKTYAIEKVFRARKYARSRPKELWECSFDIISPSVGGLAPDAEVISVVSEIISQFPSLSSQKYEVRINHLLALESILTYCGLDESHHTEALNALASCSGLSKTQLHNCINSLPLNAQQVASLLTFIQVQGPLSRVQTSLRSITRNKHSPASSRARQALHELEALRKNLDSLNLTLPVVFDLGYSHKHHNHYNGIIFKVSAEIQKKKRTAIDVLAIGGRYDKLLTTMSVFNMYDRDGEQKLTPKHAVGVSIAVEKIVGSVMEENNLPSSTSMSGSNDPTVTSQLSHHAAVDVLVCSVGRTLLLHERLRVVRSLWEASIAAETVYDRGAMESMQEVQEKAQERGIRHLVFLSEKESGSPLVRSLTHDPLLRSTERRVPLDQLVDYLLKRLKEVESTVSMKSSSSGFSASHESSTVTMTNNSARQISIEVVPPEKLASHLRKRYESEVMTKLTSLPMMSHIQSKAQICALAVELPSPVMKSMGCALDLSHSEEEFNSSLAHHLHTVSKYKKYLSYIAERICEAKFEREMTFIILYSYKDHGYCLLI

Target ORF information:

RefSeq Version	XM_026836279.1
Organism	Ciona intestinalis(vase tunicate)
Definition	Ciona intestinalis eIF-2-alpha kinase GCN2 (LOC100187226), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026836279.1

ORF Insert Sequence:

ATGACGATGA CGGAAAGTGA ATCCTATCAA GAAAGGCAGG AAAATGAACT TGAATTGCTT 
CACTCCATAT TTGGTGAGGA GATTGAAGAT CTGAGGAAAT TGGACAAATG GAAGGTGGTC 
CGTCCTCCAG AGATCATTCT CTATCTTCAT CCACAGGAAG GGATGAGCGG AAAGAAAGAA 
ACTTTTACCA AAATTGACCT CAAAATAAAA TTTACACCAA CTTACCCAGA CAGTTTCCCC 
GAGTGTAAAC TGGAGAATGC GAAAGGTTTA TCAGAAGATG CCATAAATGA TTTGGAATCT 
GGGATTAAAA ATCTCTACAA GTCATTACAG GGTGAAGTAA TGGTGCTCGA AATTGCTCAG 
TTTGTTAAGG AGTTCCTGCA TACACACAAT GTCCCACAAT GTAACTCGGT GTACGAGGAA 
ATGGTAAAAA ACAAACAAAG ACAGCTTGAA AAAGCTGCAC AGGAAGAGGA AAAGAAAAAG 
GAAATTGAAA GAAGAAAAAA GGAATTAAAG AGGAAAGAGA TAGAAGAAGA ACTAGCAAGG 
AGGCAAAACG AAGAAAAACA AATTCGTAAA CTTATGAGGG AGGGCAGAGA TCAGAACCAA 
TCATCTCAAG ACCACACACG CCGACTTTCT GAAGATATTC CGAACGGCAA TGGCAGTCAT 
GGGAAGTCTA CAGGAGTCGT GGATTTAAAA ATCAATGGAA AACTTCCACG AATAATACAT 
AAAGGGCAAA CATTAGACTA CATTGGAAGA TCCACACAAA AACTTAAAAA GATCCAGAAG 
CATATCGAGA TCTGCCATTT AAACACCACC AGTAGTGGTA GCAATGGATC AACAGTATAC 
GCGGGCATGG ATGCAAACAC AGGTGAACTA GTTGCAGTTC ATTACTGGAA CATACCATGT 
CCTATGAGGA ATTCAATGCT CACTGACGCA GAACGAGCTA AACTCGATAA ATATATGAAA 
ACAATTAACA CAATTGAGCA GGAGTTGAAT TCTTTGGTGA AATTCTCCCA CAAACACCTA 
GTGCCCTACC TTGCAATGAG ATATAGCCAT GTTGAAAGTA GTATTGAACT TCATATTGTG 
GAGAAATATA TAACAGGTGC AAGCCTTCAA CAAAAACTCA CAAGTAGAGA CAGAGTTGGA 
ATTGACGCTG TTAGAACTTA TTCTGAGCAA CTTTTATCTG CGCTTGACTG CCTACATAAA 
AAGTCTGTTG TACATAAGAA TCTTAAGGCG AGCAGTGTTT TCTTAAGCAC CAAGGGATCC 
ATCAAGCTTG CAGATTACAG CATAGAGAAA AGAGTAATAG ACTTACATAA TGAGTCCCGT 
ATTGAACAGC CTGGTGTTCG ATTCAGTGAA GATCGAATTC CTGCTCGAAA TACAAAGAAA 
GGAGATATTT TGCGGTTGGG TATGTTGATA CTGTCACTTG CACAAGGACA AACAATACAG 
GATTATCCTC CAGAAATACC AGCGAGCTTT CCACTGGTTT TCCAAAACTT TCTTGAGAAA 
TGTCTTGTGG TTGAAGACAG GCTTCGTTAT ACAACATCTG AACTCTTGGA TCATGAATTT 
ATCAAGCCAA GTGTCACCAG TCCTATCCTT GACATCGATC TTCCTTTGGA GCAAAATCCG 
AGTCCGTTTG ATGAAGATAA GATGGATGAT GTCCTTGCAA CATCCGATGC TCCAAGACCA 
ACTCTCTCTC GTCTCCACTC GGAGTTTGAG AAGTTAGAAT GGCTTGGGAA GGGAGGGTAT 
GGGGACGTGG TGAAGGTAAA GAACATAATA GACGGACGAG ATTACGCAAT CAAACGTATT 
CTACTGAACC CACATCGATC CCTGTTTAAT AAAAAGATAA CGAGAGAAGT GAAACTTTTG 
TCTCGGTTAA ACCATGAGAA TGTTGTCAGA TATTACAACT CGTGGATTGA AACTGTTGAA 
TGTTGGGAAG ATGACGTTTC AACTAATTCA TCTAACCCAA CAACAACAAC AACAACAAAT 
GAAACAAAAT CAAACGTATC AGATCGTATC AATTCACTTG GTTTTTATGA TAATATTGAG 
GTTCTAGCGC CACCTCCTGT CGCATCTAGC GTGTCAGATT GGAGCGTTTC ATTGGAGGAG 
GTGAGTCGTT GTCGTGACGA CGAGGAAGAT GATGACGAAG ATGTGTTCAA GACTATCTAT 
GTTGACGAGG ATGACTCCGA GAGCATCAAG TTTGATCACA GTGATGAAGA AGAAGATGAG 
ATGGTGGAAG AATCAACTAA TGATGGCAAA ACGTCTCGGA GTGAAGAAAC CACCGTCACT 
AGTTCCGCGT CATCAAAATC GCTTGTGACT CAGCGATTTC TATACATACA GATGGAATAT 
TGTGAGAAGA GCACACTGCG TCAAACCATT GATGCTGGCG TTTGTAATGA ACCTGAGAGA 
ATGTGGAGAT TATTCAGGGA AATTCTTGAA GGATTGGTTC ACGTACATGA GCAAGGCATG 
ATCCATAGAG ATCTAAAACC ACAGAATATT TTCCTCGATA AAAACGACCA TGTTAAGATT 
GGTGATTTTG GTCTTGCTAC TGCACAAGCT AGAGTGACAC TAGCTGACAG CTCTGAAGAG 
ATTTCAAAAT CCGAAACTGG AGACAGTGTA TATTCTGGTG TGGTGGGAAC TGCATTATAC 
GTGGCACCTG AACTTATGGT TACTGGTAAA AGATCACTGT ACAGTCAAAA GGTTGACATC 
TACAGTGTTG GAATCATTTT CTTTGAAATG TCATTTCCTG GTATCCAAAC TAAGATGGAA 
CGAGTCAAAG TTCTTGGGAA CCTTAGAACT TCTTCCATAA CCTTCCCATC TGACTTTGAT 
GAAGCTGCCA TGCCTCGTGA AGCTGAGATA ACTCGATGGT GTTTGGATCA CGTGCCAAAC 
AAACGACCAA CTGCATCTGA ACTTCTAAAG TGTAGATTAG TTCCAACTCC TGTACTAGAA 
GAATCCAATG TACTTGAAGC AGTTCGTGAA ATGTTAAGCA ATCCAGACTC AAAGCCGTAT 
AGATTAGTTA TGGATGAAGT TTTCAACCAA CCAGAAAACC CAACTTCATC TGTTATATAC 
GACATTGAAA TGCATAAGAA ACATTTTTCT GAGACACAGA ATGTCGCACA ACAGCAAACA 
TTTGACATTA TATCTTCCCT ACTACGTTGC CATGGAGCTG TGCGGGTCAA TCCTCCGCTC 
TTGTTACCTA AATGTGGTTC AGAGAATGAT GCAAATGAAG CTGCAGTGCT AATAGGGGTT 
GGCGGTAATG TCGTATCACT GCCTTATATT CTAAGGATTC CACTGGCTCG TTTTATTCTG 
TGTAAAGGAA TCACAAACAT TAAAACTTAC GCCATTGAAA AAGTTTTCCG AGCAAGAAAA 
TATGCGAGGT CCCGACCTAA GGAATTATGG GAATGTTCGT TTGACATCAT TTCCCCCTCA 
GTGGGAGGTC TCGCTCCCGA CGCGGAAGTG ATCTCTGTTG TTTCCGAGAT CATCTCCCAA 
TTCCCATCTC TCTCCTCCCA GAAGTACGAG GTTCGGATCA ACCACTTATT GGCGCTAGAG 
TCAATACTAA CATACTGTGG TCTCGATGAA AGCCACCACA CGGAGGCGCT CAATGCACTC 
GCTTCATGCA GTGGCCTCTC TAAAACACAG CTCCACAACT GCATCAACAG CTTGCCTCTC 
AATGCTCAAC AGGTTGCTTC CCTTCTTACA TTCATCCAAG TCCAAGGCCC GCTGTCCCGG 
GTTCAAACCT CGCTGCGGTC GATTACCCGG AACAAGCACT CACCCGCGTC GTCCAGAGCA 
CGACAGGCGC TCCACGAACT CGAAGCTCTT CGGAAAAATC TTGATTCTTT AAACTTGACA 
CTACCTGTTG TGTTTGATCT TGGTTATTCT CATAAACACC ACAACCATTA TAATGGCATC 
ATATTTAAAG TTTCAGCGGA AATCCAAAAG AAAAAAAGGA CAGCAATTGA TGTGTTGGCC 
ATCGGTGGTC GATATGATAA ACTGCTCACA ACCATGTCTG TATTCAACAT GTATGACAGA 
GATGGGGAAC AGAAGTTAAC ACCTAAGCAT GCTGTGGGTG TTAGTATTGC TGTGGAGAAA 
ATTGTAGGAT CCGTAATGGA AGAAAACAAT CTTCCCAGCT CAACAAGTAT GAGTGGCAGC 
AACGACCCCA CTGTGACATC ACAATTATCC CACCACGCTG CAGTTGACGT GTTGGTTTGT 
TCTGTCGGGC GAACTCTTCT GTTGCACGAA CGATTGAGGG TTGTAAGATC TCTATGGGAG 
GCCTCGATAG CAGCAGAAAC CGTTTATGAT CGCGGAGCAA TGGAATCAAT GCAAGAAGTG 
CAGGAGAAAG CTCAAGAAAG GGGGATCCGC CATCTTGTAT TTTTAAGCGA GAAAGAAAGT 
GGCTCCCCGT TAGTACGATC ATTGACGCAC GACCCGCTGT TACGCTCTAC TGAACGCAGA 
GTACCGCTGG ATCAGTTGGT GGATTATTTG TTAAAACGAT TGAAAGAAGT TGAATCAACT 
GTATCAATGA AATCTTCCTC ATCGGGGTTC TCTGCATCAC ACGAGTCCTC CACTGTTACC 
ATGACGAACA ATAGTGCTCG TCAAATCAGC ATCGAGGTTG TTCCTCCCGA GAAACTTGCC 
TCACATTTAA GGAAGAGATA TGAGTCTGAA GTTATGACCA AACTTACAAG TCTACCTATG 
ATGTCCCACA TTCAATCTAA AGCTCAAATT TGTGCCCTTG CGGTTGAACT GCCATCACCA 
GTTATGAAGA GCATGGGATG TGCTCTTGAT CTTTCTCACT CTGAGGAAGA GTTTAACTCT 
TCTCTTGCCC ATCATCTACA CACTGTATCC AAGTATAAAA AATACTTGTC CTATATCGCT 
GAGAGAATTT GCGAGGCAAA ATTCGAACGA GAAATGACTT TCATCATTCT TTACAGCTAC 
AAAGACCATG GCTACTGTCT TTTGATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100187226 ( XM_026836280.1 ) cDNA ORF clone, Ciona intestinalis(vase tunicate) -> XP_026692081.1 Ciona intestinalis eIF-2-alpha kinase GCN2 (LOC100187226), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCi153693
Clone ID Related Accession (Same CDS sequence)	XM_026836280.1
Accession Version	XM_026836280.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-21
Organism	Ciona intestinalis(vase tunicate)
Product	eIF-2-alpha kinase GCN2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_020175.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ciona intestinalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGATGA CGGAAAGTGA ATCCTATCAA GAAAGGCAGG AAAATGAACT TGAATTGCTT 
CACTCCATAT TTGGTGAGGA GATTGAAGAT CTGAGGAAAT TGGACAAATG GAAGGTGGTC 
CGTCCTCCAG AGATCATTCT CTATCTTCAT CCACAGGAAG GGATGAGCGG AAAGAAAGAA 
ACTTTTACCA AAATTGACCT CAAAATAAAA TTTACACCAA CTTACCCAGA CAGTTTCCCC 
GAGTGTAAAC TGGAGAATGC GAAAGGTTTA TCAGAAGATG CCATAAATGA TTTGGAATCT 
GGGATTAAAA ATCTCTACAA GTCATTACAG GGTGAAGTAA TGGTGCTCGA AATTGCTCAG 
TTTGTTAAGG AGTTCCTGCA TACACACAAT GTCCCACAAT GTAACTCGGT GTACGAGGAA 
ATGGTAAAAA ACAAACAAAG ACAGCTTGAA AAAGCTGCAC AGGAAGAGGA AAAGAAAAAG 
GAAATTGAAA GAAGAAAAAA GGAATTAAAG AGGAAAGAGA TAGAAGAAGA ACTAGCAAGG 
AGGCAAAACG AAGAAAAACA AATTCGTAAA CTTATGAGGG AGGGCAGAGA TCAGAACCAA 
TCATCTCAAG ACCACACACG CCGACTTTCT GAAGATATTC CGAACGGCAA TGGCAGTCAT 
GGGAAGTCTA CAGGAGTCGT GGATTTAAAA ATCAATGGAA AACTTCCACG AATAATACAT 
AAAGGGCAAA CATTAGACTA CATTGGAAGA TCCACACAAA AACTTAAAAA GATCCAGAAG 
CATATCGAGA TCTGCCATTT AAACACCACC AGTAGTGGTA GCAATGGATC AACAGTATAC 
GCGGGCATGG ATGCAAACAC AGGTGAACTA GTTGCAGTTC ATTACTGGAA CATACCATGT 
CCTATGAGGA ATTCAATGCT CACTGACGCA GAACGAGCTA AACTCGATAA ATATATGAAA 
ACAATTAACA CAATTGAGCA GGAGTTGAAT TCTTTGGTGA AATTCTCCCA CAAACACCTA 
GTGCCCTACC TTGCAATGAG ATATAGCCAT GTTGAAAGTA GTATTGAACT TCATATTGTG 
GAGAAATATA TAACAGGTGC AAGCCTTCAA CAAAAACTCA CAAGTAGAGA CAGAGTTGGA 
ATTGACGCTG TTAGAACTTA TTCTGAGCAA CTTTTATCTG CGCTTGACTG CCTACATAAA 
AAGTCTGTTG TACATAAGAA TCTTAAGGCG AGCAGTGTTT TCTTAAGCAC CAAGGGATCC 
ATCAAGCTTG CAGATTACAG CATAGAGAAA AGAGTAATAG ACTTACATAA TGAGTCCCGT 
ATTGAACAGC CTGGTGTTCG ATTCAGTGAA GATCGAATTC CTGCTCGAAA TACAAAGAAA 
GGAGATATTT TGCGGTTGGG TATGTTGATA CTGTCACTTT CACAAGGACA AACAATACAG 
GATTATCCCC CAGAAATACC TGCGAGCTTT CCACTGGTTT TCCAAAACTT TCTTGAGAAA 
TGTCTTGTGG TTGAAGACAG GCTTCGTTAT ACAACATCTG AACTCTTGGA TCATGAATTT 
ATCAAGCCAA GTGTCACCAG TCCTATCCTT GACATCGATC TTCCTTTGGA GCAAAATCCG 
AGTCCGTTTG ATGAAGATAA GATGGATGAT GTCCTCGCAA CATCCGATGC TCCAAGACCA 
ACTCTCTCTC GTCTCCACTC GGAATTTGAG AAGTTAGAAT GGCTTGGGAA GGGAGGGTAT 
GGGGACGTGG TGAAGGTAAA GAACATAATA GACGGACGAG ATTACGCAAT CAAACGTATT 
CTACTGAACC CACATCGATC CCTGTTTAAT AAAAAGATAA CGAGAGAAGT GAAACTTTTG 
TCTCGGTTAA ACCATGAGAA TGTTGTCAGA TATTACAACT CGTGGATTGA AACTGTTGAA 
TGTTGGGAAG ATGACGTTTC AACTAATTCA TCTAACCCAA CAACAACAAC AACAACAAAT 
GAAACAAAAT CAAACGTATC AGATCGTATC AATTCACTTG GTTTTTATGA TAATATTGAG 
GTTCTAGCGC CACCTCCTGT CGCATCTAGC GTGTCAGATT GGAGCGTTTC ATTGGAGGAG 
GTGAGTCGTT GTCGTGACGA CGAGGAAGAT GATGACGAAG ATGTGTTCAA GACTATCTAT 
GTTGACGAGG ATGACTCCGA GAGCATCAAG TTTGATCACA GTGATGAAGA AGAAGATGAG 
ATGGTGGAAG AATCAACTAA TGATGGCAAA ACGTCTCGGA GTGAAGAAAC CACCGTCACT 
AGTTCCGCGT CATCAAAATC GCTTGTGACT CAGCGATTTC TATACATACA GATGGAATAT 
TGTGAGAAGA GCACACTGCG TCAAACCATT GATGCTGGCG TTTGTAATGA ACCTGAGAGA 
ATGTGGAGAT TATTCAGGGA AATTCTTGAA GGATTGGTTC ACGTACATGA GCAAGGCATG 
ATCCATAGAG ATCTAAAACC ACAGAATATT TTCCTCGATA AAAACGACCA TGTTAAGATT 
GGTGATTTTG GTCTTGCTAC TGCACAAGCT AGAGTGACAC TAGCTGACAG CTCTGAAGAG 
ATTTCAAAAT CCGAAACTGG AGACAGTGTA TATTCTGGTG TGGTGGGAAC TGCATTATAC 
GTGGCACCTG AACTTATGGT TACTGGTAAA AGATCACTGT ACAGTCAAAA GGTTGACATC 
TACAGTGTTG GAATCATTTT CTTTGAAATG TCATTTCCTG GTATCCAAAC TAAGATGGAA 
CGAGTCAAAG TTCTTGGGAA CCTTAGAACT TCTTCCATAA CCTTCCCATC TGACTTTGAT 
GAAGCTGCCA TGCCTCGTGA AGCTGAGATA ACTCGATGGT GTTTGGATCA CGTGCCAAAC 
AAACGACCAA CTGCATCTGA ACTTCTAAAG TGTAGATTAG TTCCAACTCC TGTACTAGAA 
GAATCCAATG TACTTGAAGC AGTTCGTGAA ATGTTAAGCA ATCCAGACTC AAAGCCGTAT 
AGATTAGTTA TGGATGAAGT TTTCAACCAA CCAGAAAACC CAACTTCATC TGTTATATAC 
GACATTGAAA TGCATAAGAA ACATTTTTCT GAGACACAGA ATGTCGCACA ACAGCAAACA 
TTTGACATTA TATCTTCCCT ACTACGTTGC CATGGAGCTG TGCGGGTCAA TCCTCCGCTC 
TTGTTACCTA AATGTGGTTC AGAGAATGAT GCAAATGAAG CTGCAGTGCT AATAGGGGTT 
GGCGGTAATG TCGTATCACT GCCTTATATT CTAAGGATTC CACTGGCTCG TTTTATTCTG 
TGTAAAGGAA TCACAAACAT TAAAACTTAC GCCATTGAAA AAGTTTTCCG AGCAAGAAAA 
TATGCGAGGT CCCGACCTAA GGAATTATGG GAATGTTCGT TTGACATCAT TTCCCCCTCA 
GTGGGAGGTC TCGCTCCCGA CGCGGAAGTG ATCTCTGTTG TTTCCGAGAT CATCTCCCAA 
TTCCCATCTC TCTCCTCCCA GAAGTACGAG GTTCGGATCA ACCACTTATT GGCGCTAGAG 
TCAATACTAA CATACTGTGG TCTCGATGAA AGCCACCACA CGGAGGCGCT CAATGCACTC 
GCTTCATGCA GTGGCCTCTC TAAAACACAG CTCCACAACT GCATCAACAG CTTGCCTCTC 
AATGCTCAAC AGGTTGCTTC CCTTCTTACA TTCATCCAAG TCCAAGGCCC GCTGTCCCGG 
GTTCAAACCT CGCTGCGGTC GATTACCCGG AACAAGCACT CACCCGCGTC GTCCAGAGCA 
CGACAGGCGC TCCACGAACT CGAAGCTCTT CGGAAAAATC TTGATTCTTT AAACTTGACA 
CTACCTGTTG TGTTTGATCT TGGTTATTCT CATAAACACC ACAACCATTA TAATGGCATC 
ATATTTAAAG TTTCAGCGGA AATCCAAAAG AAAAAAAGGA CAGCAATTGA TGTGTTGGCC 
ATCGGTGGTC GATATGATAA ACTGCTCACA ACCATGTCTG TATTCAACAT GTATGACAGA 
GATGGGGAAC AGAAGTTAAC ACCTAAGCAT GCTGTGGGTG TTAGTATTGC TGTGGAGAAA 
ATTGTAGGAT CCGTAATGGA AGAAAACAAT CTTCCCAGCT CAACAAGTAT GAGTGGCAGC 
AACGACCCCA CTGTGACATC ACAATTATCC CACCACGCTG CAGTTGACGT GTTGGTTTGT 
TCTGTCGGGC GAACTCTTCT GTTGCACGAA CGATTGAGGG TTGTAAGATC TCTATGGGAG 
GCCTCGATAG CAGCAGAAAC CGTTTATGAT CGCGGAGCAA TGGAATCAAT GCAAGAAGTG 
CAGGAGAAAG CTCAAGAAAG GGGGATCCGC CATCTTGTAT TTTTAAGCGA GAAAGAAAGT 
GGCTCCCCGT TAGTACGATC ATTGACGCAC GACCCGCTGT TACGCTCTAC TGAACGCAGA 
GTACCGCTGG ATCAGTTGGT GGATTATTTG TTAAAACGAT TGAAAGAAGT TGAATCAACT 
GTATCAATGA AATCTTCCTC ATCGGGGTTC TCTGCATCAC ACGAGTCCTC CACTGTTACC 
ATGACGAACA ATAGTGCTCG TCAAATCAGC ATCGAGGTTG TTCCTCCCGA GAAACTTGCC 
TCACATTTAA GGAAGAGATA TGAGTCTGAA GTTATGACCA AACTTACAAG TCTACCTATG 
ATGTCCCACA TTCAATCTAA AGCTCAAATT TGTGCCCTTG CGGTTGAACT GCCATCACCA 
GTTATGAAGA GCATGGGATG TGCTCTTGAT CTTTCTCACT CTGAGGAAGA GTTTAACTCT 
TCTCTTGCCC ATCATCTACA CACTGTATCC AAGTATAAAA AATACTTGTC CTATATCGCT 
GAGAGAATTT GCGAGGCAAA ATTCGAACGA GAAATGACTT TCATCATTCT TTACAGCTAC 
AAAGACCATG GCTACTGTCT TTTGATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026692081.1
CDS	107..5056
Translation	MTMTESESYQERQENELELLHSIFGEEIEDLRKLDKWKVVRPPEIILYLHPQEGMSGKKETFTKIDLKIKFTPTYPDSFPECKLENAKGLSEDAINDLESGIKNLYKSLQGEVMVLEIAQFVKEFLHTHNVPQCNSVYEEMVKNKQRQLEKAAQEEEKKKEIERRKKELKRKEIEEELARRQNEEKQIRKLMREGRDQNQSSQDHTRRLSEDIPNGNGSHGKSTGVVDLKINGKLPRIIHKGQTLDYIGRSTQKLKKIQKHIEICHLNTTSSGSNGSTVYAGMDANTGELVAVHYWNIPCPMRNSMLTDAERAKLDKYMKTINTIEQELNSLVKFSHKHLVPYLAMRYSHVESSIELHIVEKYITGASLQQKLTSRDRVGIDAVRTYSEQLLSALDCLHKKSVVHKNLKASSVFLSTKGSIKLADYSIEKRVIDLHNESRIEQPGVRFSEDRIPARNTKKGDILRLGMLILSLSQGQTIQDYPPEIPASFPLVFQNFLEKCLVVEDRLRYTTSELLDHEFIKPSVTSPILDIDLPLEQNPSPFDEDKMDDVLATSDAPRPTLSRLHSEFEKLEWLGKGGYGDVVKVKNIIDGRDYAIKRILLNPHRSLFNKKITREVKLLSRLNHENVVRYYNSWIETVECWEDDVSTNSSNPTTTTTTNETKSNVSDRINSLGFYDNIEVLAPPPVASSVSDWSVSLEEVSRCRDDEEDDDEDVFKTIYVDEDDSESIKFDHSDEEEDEMVEESTNDGKTSRSEETTVTSSASSKSLVTQRFLYIQMEYCEKSTLRQTIDAGVCNEPERMWRLFREILEGLVHVHEQGMIHRDLKPQNIFLDKNDHVKIGDFGLATAQARVTLADSSEEISKSETGDSVYSGVVGTALYVAPELMVTGKRSLYSQKVDIYSVGIIFFEMSFPGIQTKMERVKVLGNLRTSSITFPSDFDEAAMPREAEITRWCLDHVPNKRPTASELLKCRLVPTPVLEESNVLEAVREMLSNPDSKPYRLVMDEVFNQPENPTSSVIYDIEMHKKHFSETQNVAQQQTFDIISSLLRCHGAVRVNPPLLLPKCGSENDANEAAVLIGVGGNVVSLPYILRIPLARFILCKGITNIKTYAIEKVFRARKYARSRPKELWECSFDIISPSVGGLAPDAEVISVVSEIISQFPSLSSQKYEVRINHLLALESILTYCGLDESHHTEALNALASCSGLSKTQLHNCINSLPLNAQQVASLLTFIQVQGPLSRVQTSLRSITRNKHSPASSRARQALHELEALRKNLDSLNLTLPVVFDLGYSHKHHNHYNGIIFKVSAEIQKKKRTAIDVLAIGGRYDKLLTTMSVFNMYDRDGEQKLTPKHAVGVSIAVEKIVGSVMEENNLPSSTSMSGSNDPTVTSQLSHHAAVDVLVCSVGRTLLLHERLRVVRSLWEASIAAETVYDRGAMESMQEVQEKAQERGIRHLVFLSEKESGSPLVRSLTHDPLLRSTERRVPLDQLVDYLLKRLKEVESTVSMKSSSSGFSASHESSTVTMTNNSARQISIEVVPPEKLASHLRKRYESEVMTKLTSLPMMSHIQSKAQICALAVELPSPVMKSMGCALDLSHSEEEFNSSLAHHLHTVSKYKKYLSYIAERICEAKFEREMTFIILYSYKDHGYCLLI

Target ORF information:

RefSeq Version	XM_026836280.1
Organism	Ciona intestinalis(vase tunicate)
Definition	Ciona intestinalis eIF-2-alpha kinase GCN2 (LOC100187226), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026836280.1

ORF Insert Sequence:

ATGACGATGA CGGAAAGTGA ATCCTATCAA GAAAGGCAGG AAAATGAACT TGAATTGCTT 
CACTCCATAT TTGGTGAGGA GATTGAAGAT CTGAGGAAAT TGGACAAATG GAAGGTGGTC 
CGTCCTCCAG AGATCATTCT CTATCTTCAT CCACAGGAAG GGATGAGCGG AAAGAAAGAA 
ACTTTTACCA AAATTGACCT CAAAATAAAA TTTACACCAA CTTACCCAGA CAGTTTCCCC 
GAGTGTAAAC TGGAGAATGC GAAAGGTTTA TCAGAAGATG CCATAAATGA TTTGGAATCT 
GGGATTAAAA ATCTCTACAA GTCATTACAG GGTGAAGTAA TGGTGCTCGA AATTGCTCAG 
TTTGTTAAGG AGTTCCTGCA TACACACAAT GTCCCACAAT GTAACTCGGT GTACGAGGAA 
ATGGTAAAAA ACAAACAAAG ACAGCTTGAA AAAGCTGCAC AGGAAGAGGA AAAGAAAAAG 
GAAATTGAAA GAAGAAAAAA GGAATTAAAG AGGAAAGAGA TAGAAGAAGA ACTAGCAAGG 
AGGCAAAACG AAGAAAAACA AATTCGTAAA CTTATGAGGG AGGGCAGAGA TCAGAACCAA 
TCATCTCAAG ACCACACACG CCGACTTTCT GAAGATATTC CGAACGGCAA TGGCAGTCAT 
GGGAAGTCTA CAGGAGTCGT GGATTTAAAA ATCAATGGAA AACTTCCACG AATAATACAT 
AAAGGGCAAA CATTAGACTA CATTGGAAGA TCCACACAAA AACTTAAAAA GATCCAGAAG 
CATATCGAGA TCTGCCATTT AAACACCACC AGTAGTGGTA GCAATGGATC AACAGTATAC 
GCGGGCATGG ATGCAAACAC AGGTGAACTA GTTGCAGTTC ATTACTGGAA CATACCATGT 
CCTATGAGGA ATTCAATGCT CACTGACGCA GAACGAGCTA AACTCGATAA ATATATGAAA 
ACAATTAACA CAATTGAGCA GGAGTTGAAT TCTTTGGTGA AATTCTCCCA CAAACACCTA 
GTGCCCTACC TTGCAATGAG ATATAGCCAT GTTGAAAGTA GTATTGAACT TCATATTGTG 
GAGAAATATA TAACAGGTGC AAGCCTTCAA CAAAAACTCA CAAGTAGAGA CAGAGTTGGA 
ATTGACGCTG TTAGAACTTA TTCTGAGCAA CTTTTATCTG CGCTTGACTG CCTACATAAA 
AAGTCTGTTG TACATAAGAA TCTTAAGGCG AGCAGTGTTT TCTTAAGCAC CAAGGGATCC 
ATCAAGCTTG CAGATTACAG CATAGAGAAA AGAGTAATAG ACTTACATAA TGAGTCCCGT 
ATTGAACAGC CTGGTGTTCG ATTCAGTGAA GATCGAATTC CTGCTCGAAA TACAAAGAAA 
GGAGATATTT TGCGGTTGGG TATGTTGATA CTGTCACTTT CACAAGGACA AACAATACAG 
GATTATCCCC CAGAAATACC TGCGAGCTTT CCACTGGTTT TCCAAAACTT TCTTGAGAAA 
TGTCTTGTGG TTGAAGACAG GCTTCGTTAT ACAACATCTG AACTCTTGGA TCATGAATTT 
ATCAAGCCAA GTGTCACCAG TCCTATCCTT GACATCGATC TTCCTTTGGA GCAAAATCCG 
AGTCCGTTTG ATGAAGATAA GATGGATGAT GTCCTCGCAA CATCCGATGC TCCAAGACCA 
ACTCTCTCTC GTCTCCACTC GGAATTTGAG AAGTTAGAAT GGCTTGGGAA GGGAGGGTAT 
GGGGACGTGG TGAAGGTAAA GAACATAATA GACGGACGAG ATTACGCAAT CAAACGTATT 
CTACTGAACC CACATCGATC CCTGTTTAAT AAAAAGATAA CGAGAGAAGT GAAACTTTTG 
TCTCGGTTAA ACCATGAGAA TGTTGTCAGA TATTACAACT CGTGGATTGA AACTGTTGAA 
TGTTGGGAAG ATGACGTTTC AACTAATTCA TCTAACCCAA CAACAACAAC AACAACAAAT 
GAAACAAAAT CAAACGTATC AGATCGTATC AATTCACTTG GTTTTTATGA TAATATTGAG 
GTTCTAGCGC CACCTCCTGT CGCATCTAGC GTGTCAGATT GGAGCGTTTC ATTGGAGGAG 
GTGAGTCGTT GTCGTGACGA CGAGGAAGAT GATGACGAAG ATGTGTTCAA GACTATCTAT 
GTTGACGAGG ATGACTCCGA GAGCATCAAG TTTGATCACA GTGATGAAGA AGAAGATGAG 
ATGGTGGAAG AATCAACTAA TGATGGCAAA ACGTCTCGGA GTGAAGAAAC CACCGTCACT 
AGTTCCGCGT CATCAAAATC GCTTGTGACT CAGCGATTTC TATACATACA GATGGAATAT 
TGTGAGAAGA GCACACTGCG TCAAACCATT GATGCTGGCG TTTGTAATGA ACCTGAGAGA 
ATGTGGAGAT TATTCAGGGA AATTCTTGAA GGATTGGTTC ACGTACATGA GCAAGGCATG 
ATCCATAGAG ATCTAAAACC ACAGAATATT TTCCTCGATA AAAACGACCA TGTTAAGATT 
GGTGATTTTG GTCTTGCTAC TGCACAAGCT AGAGTGACAC TAGCTGACAG CTCTGAAGAG 
ATTTCAAAAT CCGAAACTGG AGACAGTGTA TATTCTGGTG TGGTGGGAAC TGCATTATAC 
GTGGCACCTG AACTTATGGT TACTGGTAAA AGATCACTGT ACAGTCAAAA GGTTGACATC 
TACAGTGTTG GAATCATTTT CTTTGAAATG TCATTTCCTG GTATCCAAAC TAAGATGGAA 
CGAGTCAAAG TTCTTGGGAA CCTTAGAACT TCTTCCATAA CCTTCCCATC TGACTTTGAT 
GAAGCTGCCA TGCCTCGTGA AGCTGAGATA ACTCGATGGT GTTTGGATCA CGTGCCAAAC 
AAACGACCAA CTGCATCTGA ACTTCTAAAG TGTAGATTAG TTCCAACTCC TGTACTAGAA 
GAATCCAATG TACTTGAAGC AGTTCGTGAA ATGTTAAGCA ATCCAGACTC AAAGCCGTAT 
AGATTAGTTA TGGATGAAGT TTTCAACCAA CCAGAAAACC CAACTTCATC TGTTATATAC 
GACATTGAAA TGCATAAGAA ACATTTTTCT GAGACACAGA ATGTCGCACA ACAGCAAACA 
TTTGACATTA TATCTTCCCT ACTACGTTGC CATGGAGCTG TGCGGGTCAA TCCTCCGCTC 
TTGTTACCTA AATGTGGTTC AGAGAATGAT GCAAATGAAG CTGCAGTGCT AATAGGGGTT 
GGCGGTAATG TCGTATCACT GCCTTATATT CTAAGGATTC CACTGGCTCG TTTTATTCTG 
TGTAAAGGAA TCACAAACAT TAAAACTTAC GCCATTGAAA AAGTTTTCCG AGCAAGAAAA 
TATGCGAGGT CCCGACCTAA GGAATTATGG GAATGTTCGT TTGACATCAT TTCCCCCTCA 
GTGGGAGGTC TCGCTCCCGA CGCGGAAGTG ATCTCTGTTG TTTCCGAGAT CATCTCCCAA 
TTCCCATCTC TCTCCTCCCA GAAGTACGAG GTTCGGATCA ACCACTTATT GGCGCTAGAG 
TCAATACTAA CATACTGTGG TCTCGATGAA AGCCACCACA CGGAGGCGCT CAATGCACTC 
GCTTCATGCA GTGGCCTCTC TAAAACACAG CTCCACAACT GCATCAACAG CTTGCCTCTC 
AATGCTCAAC AGGTTGCTTC CCTTCTTACA TTCATCCAAG TCCAAGGCCC GCTGTCCCGG 
GTTCAAACCT CGCTGCGGTC GATTACCCGG AACAAGCACT CACCCGCGTC GTCCAGAGCA 
CGACAGGCGC TCCACGAACT CGAAGCTCTT CGGAAAAATC TTGATTCTTT AAACTTGACA 
CTACCTGTTG TGTTTGATCT TGGTTATTCT CATAAACACC ACAACCATTA TAATGGCATC 
ATATTTAAAG TTTCAGCGGA AATCCAAAAG AAAAAAAGGA CAGCAATTGA TGTGTTGGCC 
ATCGGTGGTC GATATGATAA ACTGCTCACA ACCATGTCTG TATTCAACAT GTATGACAGA 
GATGGGGAAC AGAAGTTAAC ACCTAAGCAT GCTGTGGGTG TTAGTATTGC TGTGGAGAAA 
ATTGTAGGAT CCGTAATGGA AGAAAACAAT CTTCCCAGCT CAACAAGTAT GAGTGGCAGC 
AACGACCCCA CTGTGACATC ACAATTATCC CACCACGCTG CAGTTGACGT GTTGGTTTGT 
TCTGTCGGGC GAACTCTTCT GTTGCACGAA CGATTGAGGG TTGTAAGATC TCTATGGGAG 
GCCTCGATAG CAGCAGAAAC CGTTTATGAT CGCGGAGCAA TGGAATCAAT GCAAGAAGTG 
CAGGAGAAAG CTCAAGAAAG GGGGATCCGC CATCTTGTAT TTTTAAGCGA GAAAGAAAGT 
GGCTCCCCGT TAGTACGATC ATTGACGCAC GACCCGCTGT TACGCTCTAC TGAACGCAGA 
GTACCGCTGG ATCAGTTGGT GGATTATTTG TTAAAACGAT TGAAAGAAGT TGAATCAACT 
GTATCAATGA AATCTTCCTC ATCGGGGTTC TCTGCATCAC ACGAGTCCTC CACTGTTACC 
ATGACGAACA ATAGTGCTCG TCAAATCAGC ATCGAGGTTG TTCCTCCCGA GAAACTTGCC 
TCACATTTAA GGAAGAGATA TGAGTCTGAA GTTATGACCA AACTTACAAG TCTACCTATG 
ATGTCCCACA TTCAATCTAA AGCTCAAATT TGTGCCCTTG CGGTTGAACT GCCATCACCA 
GTTATGAAGA GCATGGGATG TGCTCTTGAT CTTTCTCACT CTGAGGAAGA GTTTAACTCT 
TCTCTTGCCC ATCATCTACA CACTGTATCC AAGTATAAAA AATACTTGTC CTATATCGCT 
GAGAGAATTT GCGAGGCAAA ATTCGAACGA GAAATGACTT TCATCATTCT TTACAGCTAC 
AAAGACCATG GCTACTGTCT TTTGATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100187226 ( XM_018813629.2 ) cDNA ORF clone, Ciona intestinalis(vase tunicate) -> XP_018669174.1 Ciona intestinalis eIF-2-alpha kinase GCN2 (LOC100187226), transcript variant X1, mRNA.

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CloneID	OCi70880
Clone ID Related Accession (Same CDS sequence)	XM_018813629.2 , XM_018813629.1
Accession Version	XM_018813629.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-21
Organism	Ciona intestinalis(vase tunicate)
Product	eIF-2-alpha kinase GCN2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_020175.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Oct 22, 2018 this sequence version replaced XM_018813629.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ciona intestinalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGATGA CGGAAAGTGA ATCCTATCAA GAAAGGCAGG AAAATGAACT TGAATTGCTT 
CACTCCATAT TTGGTGAGGA GATTGAAGAT CTGAGGAAAT TGGACAAATG GAAGGTGGTC 
CGTCCTCCAG AGATCATTCT CTATCTTCAT CCACAGGAAG GGATGAGCGG AAAGAAAGAA 
ACTTTTACCA AAATTGACCT CAAAATAAAA TTTACACCAA CTTACCCAGA CAGTTTCCCC 
GAGTGTAAAC TGGAGAATGC GAAAGGTTTA TCAGAAGATG CCATAAATGA TTTGGAATCT 
GGGATTAAAA ATCTCTACAA GTCATTACAG GGTGAAGTAA TGGTGCTCGA AATTGCTCAG 
TTTGTTAAGG AGTTCCTGCA TACACACAAT GTCCCACAAT GTAACTCGGT GTACGAGGAA 
ATGGTAAAAA ACAAACAAAG ACAGCTTGAA AAAGCTGCAC AGGAAGAGGA AAAGAAAAAG 
GAAATTGAAA GAAGAAAAAA GGAATTAAAG AGGAAAGAGA TAGAAGAAGA ACTAGCAAGG 
AGGCAAAACG AAGAAAAACA AATTCGTAAA CTTATGAGGG AGGGCAGAGA TCAGAACCAA 
TCATCTCAAG ACCACACACG CCGACTTTCT GAAGATATTC CGAACGGCAA TGGCAGTCAT 
GGGAAGTCTA CAGGAGTCGT GGATTTAAAA ATCAATGGAA AACTTCCACG AATAATACAT 
AAAGGGCAAA CATTAGACTA CATTGGAAGA TCCACACAAA AACTTAAAAA GATCCAGAAG 
CATATCGAGA TCTGCCATTT AAACACCACC AGTAGTGGTA GCAATGGATC AACAGTATAC 
GCGGGCATGG ATGCAAACAC AGGTGAACTA GTTGCAGTTC ATTACTGGAA CATACCATGT 
CCTATGAGGA ATTCAATGCT CACTGACGCA GAACGAGCTA AACTCGATAA ATATATGAAA 
ACAATTAACA CAATTGAGCA GGAGTTGAAT TCTTTGGTGA AATTCTCCCA CAAACACCTA 
GTGCCCTACC TTGCAATGAG ATATAGCCAT GTTGAAAGTA GTATTGAACT TCATATTGTG 
GAGAAATATA TAACAGGTGC AAGCCTTCAA CAAAAACTCA CAAGTAGAGA CAGAGTTGGA 
ATTGACGCTG TTAGAACTTA TTCTGAGCAA CTTTTATCTG CGCTTGACTG CCTACATAAA 
AAGTCTGTTG TACATAAGAA TCTTAAGGCG AGCAGTGTTT TCTTAAGCAC CAAGGGATCC 
ATCAAGCTTG CAGATTACAG CATAGAGAAA AGAGTAATAG ACTTACATAA TGAGTCCCGT 
ATTGAACAGC CTGGTGTTCG ATTCAGTGAA GATCGAATTC CTGCTCGAAA TACAAAGAAA 
GGAGATATTT TGCGGTTGGG TATGTTGATA CTGTCACTTG CACAAGGACA AACAATACAG 
GATTATCCTC CAGAAATACC AGCGAGCTTT CCACTGGTTT TCCAAAACTT TCTTGAGAAA 
TGTCTTGTGG TTGAAGACAG GCTTCGTTAT ACAACATCTG AACTCTTGGA TCATGAATTT 
ATCAAGCCAA GTGTCACCAG TCCTATCCTT GACATCGATC TTCCTTTGGA GCAAAATCCG 
AGTCCGTTTG ATGAAGATAA GATGGATGAT GTCCTTGCAA CATCCGATGC TCCAAGACCA 
ACTCTCTCTC GTCTCCACTC GGAGTTTGAG AAGTTAGAAT GGCTTGGGAA GGGAGGGTAT 
GGGGACGTGG TGAAGGTAAA GAACATAATA GACGGACGAG ATTACGCAAT CAAACGTATT 
CTACTGAACC CACATCGATC CCTGTTTAAT AAAAAGATAA CGAGAGAAGT GAAACTTTTG 
TCTCGGTTAA ACCATGAGAA TGTTGTCAGA TATTACAACT CGTGGATTGA AACAGTTGAA 
TGTTGGGAAG ATGATGTTTC AACAAATTCA TCTAACCCAA CAACAACAAC AACAACAACA 
AATGAAACAA AATCAAACGT ATCAGATCGT ATCAATTCAC TTGGTTTTTA TGATAATATT 
GAGGTTCTAG CGCCACCTCC TGTCGCATCT AGCGTGTCAG ATTGGAGCGT TTCATTGGAG 
GAGGTGAGTC GTTGTCGTGA CGACGAGGAA GATGATGACG AAGATGTGTT CAAGACTATC 
TATGTTGACG AGGATGACTC CGAGAGCATC AAGTTTGATC ACAGTGATGA AGAAGAAGAT 
GAGATGGTGG AAGAATCAAC TAATGATGGC AAAACGTCTC GGAGTGAAGA AACCACCGTC 
ACTAGTTCCG CGTCATCAAA ATCGCTTGTG ACTCAGCGAT TTCTATACAT ACAGATGGAA 
TATTGTGAGA AGAGCACACT GCGTCAAACC ATTGATGCTG GCGTTTGTAA TGAACCTGAG 
AGAATGTGGA GATTATTCAG GGAAATTCTT GAAGGATTGG TTCACGTACA TGAGCAAGGC 
ATGATCCATA GAGATCTAAA ACCACAGAAT ATTTTCCTCG ATAAAAACGA CCATGTTAAG 
ATTGGTGATT TTGGTCTTGC TACTGCACAA GCTAGAGTGA CACTAGCTGA CAGCTCTGAA 
GAGATTTCAA AATCCGAAAC TGGAGACAGT GTATATTCTG GTGTGGTGGG AACTGCATTA 
TACGTGGCAC CTGAACTTAT GGTTACTGGT AAAAGATCAC TGTACAGTCA AAAGGTTGAC 
ATCTACAGTG TTGGAATCAT TTTCTTTGAA ATGTCATTTC CTGGTATCCA AACTAAGATG 
GAACGAGTCA AAGTTCTTGG GAACCTTAGA ACTTCTTCCA TAACCTTCCC ATCTGACTTT 
GATGAAGCTG CCATGCCTCG TGAAGCTGAG ATAACTCGAT GGTGTTTGGA TCACGTGCCA 
AACAAACGAC CAACTGCATC TGAACTTCTA AAGTGTAGAT TAGTTCCAAC TCCTGTACTA 
GAAGAATCCA ATGTACTTGA AGCAGTTCGT GAAATGTTAA GCAATCCAGA CTCAAAGCCG 
TATAGATTAG TTATGGATGA AGTTTTCAAC CAACCAGAAA ACCCAACTTC ATCTGTTATA 
TACGACATTG AAATGCATAA GAAACATTTT TCTGAGACAC AGAATGTCGC ACAACAGCAA 
ACATTTGACA TTATATCTTC CCTACTACGT TGCCATGGAG CTGTGCGGGT CAATCCTCCG 
CTCTTGTTAC CTAAATGTGG TTCAGAGAAT GATGCAAATG AAGCTGCAGT GCTAATAGGG 
GTTGGCGGTA ATGTCGTATC ACTGCCTTAT ATTCTAAGGA TTCCACTGGC TCGTTTTATT 
CTGTGTAAAG GAATCACAAA CATTAAAACT TACGCCATTG AAAAAGTTTT CCGAGCAAGA 
AAATATGCGA GGTCCCGACC TAAGGAATTA TGGGAATGTT CGTTTGACAT CATTTCCCCC 
TCAGTGGGAG GTCTCGCTCC CGACGCGGAA GTGATCTCTG TTGTTTCCGA GATCATCTCC 
CAATTCCCAT CTCTCTCCTC CCAGAAGTAC GAGGTTCGGA TCAACCACTT ATTGGCGCTA 
GAGTCAATAC TAACATACTG TGGTCTCGAT GAAAGCCACC ACACGGAGGC GCTCAATGCA 
CTCGCTTCAT GCAGTGGCCT CTCTAAAACA CAGCTCCACA ACTGCATCAA CAGCTTGCCT 
CTCAATGCTC AACAGGTTGC TTCCCTTCTT ACATTCATCC AAGTCCAAGG CCCGCTGTCC 
CGGGTTCAAA CCTCGCTGCG GTCGATTACC CGGAACAAGC ACTCACCCGC GTCGTCCAGA 
GCACGACAGG CGCTCCACGA ACTCGAAGCT CTTCGGAAAA ATCTTGATTC TTTAAACTTG 
ACACTACCTG TTGTGTTTGA TCTTGGTTAT TCTCATAAAC ACCACAACCA TTATAATGGC 
ATCATATTTA AAGTTTCAGC GGAAATCCAA AAGAAAAAAA GGACAGCAAT TGATGTGTTG 
GCCATCGGTG GTCGATATGA TAAACTGCTC ACAACCATGT CTGTATTCAA CATGTATGAC 
AGAGATGGGG AACAGAAGTT AACACCTAAG CATGCTGTGG GTGTTAGTAT TGCTGTGGAG 
AAAATTGTAG GATCCGTAAT GGAAGAAAAC AATCTTCCCA GCTCAACAAG TATGAGTGGC 
AGCAACGACC CCACTGTGAC ATCACAATTA TCCCACCACG CTGCAGTTGA CGTGTTGGTT 
TGTTCTGTCG GGCGAACTCT TCTGTTGCAC GAACGATTGA GGGTTGTAAG ATCTCTATGG 
GAGGCCTCGA TAGCAGCAGA AACCGTTTAT GATCGCGGAG CAATGGAATC AATGCAAGAA 
GTGCAGGAGA AAGCTCAAGA AAGGGGGATC CGCCATCTTG TATTTTTAAG CGAGAAAGAA 
AGTGGCTCCC CGTTAGTACG ATCATTGACG CACGACCCGC TGTTACGCTC TACTGAACGC 
AGAGTACCGC TGGATCAGTT GGTGGATTAT TTGTTAAAAC GATTGAAAGA AGTTGAATCA 
ACTGTATCAA TGAAATCTTC CTCATCGGGG TTCTCTGCAT CACACGAGTC CTCCACTGTT 
ACCATGACGA ACAATAGTGC TCGTCAAATC AGCATCGAGG TTGTTCCTCC CGAGAAACTT 
GCCTCACATT TAAGGAAGAG ATATGAGTCT GAAGTTATGA CCAAACTTAC AAGTCTACCT 
ATGATGTCCC ACATTCAATC TAAAGCTCAA ATTTGTGCCC TTGCGGTTGA ACTGCCATCA 
CCAGTTATGA AGAGCATGGG ATGTGCTCTT GATCTTTCTC ACTCTGAGGA AGAGTTTAAC 
TCTTCTCTTG CCCATCATCT ACACACTGTA TCCAAGTATA AAAAATACTT GTCCTATATC 
GCTGAGAGAA TTTGCGAGGC AAAATTCGAA CGAGAAATGA CTTTCATCAT TCTTTACAGC 
TACAAAGACC ATGGCTACTG TCTTTTGATA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018669174.1
CDS	107..5059
Translation	MTMTESESYQERQENELELLHSIFGEEIEDLRKLDKWKVVRPPEIILYLHPQEGMSGKKETFTKIDLKIKFTPTYPDSFPECKLENAKGLSEDAINDLESGIKNLYKSLQGEVMVLEIAQFVKEFLHTHNVPQCNSVYEEMVKNKQRQLEKAAQEEEKKKEIERRKKELKRKEIEEELARRQNEEKQIRKLMREGRDQNQSSQDHTRRLSEDIPNGNGSHGKSTGVVDLKINGKLPRIIHKGQTLDYIGRSTQKLKKIQKHIEICHLNTTSSGSNGSTVYAGMDANTGELVAVHYWNIPCPMRNSMLTDAERAKLDKYMKTINTIEQELNSLVKFSHKHLVPYLAMRYSHVESSIELHIVEKYITGASLQQKLTSRDRVGIDAVRTYSEQLLSALDCLHKKSVVHKNLKASSVFLSTKGSIKLADYSIEKRVIDLHNESRIEQPGVRFSEDRIPARNTKKGDILRLGMLILSLAQGQTIQDYPPEIPASFPLVFQNFLEKCLVVEDRLRYTTSELLDHEFIKPSVTSPILDIDLPLEQNPSPFDEDKMDDVLATSDAPRPTLSRLHSEFEKLEWLGKGGYGDVVKVKNIIDGRDYAIKRILLNPHRSLFNKKITREVKLLSRLNHENVVRYYNSWIETVECWEDDVSTNSSNPTTTTTTTNETKSNVSDRINSLGFYDNIEVLAPPPVASSVSDWSVSLEEVSRCRDDEEDDDEDVFKTIYVDEDDSESIKFDHSDEEEDEMVEESTNDGKTSRSEETTVTSSASSKSLVTQRFLYIQMEYCEKSTLRQTIDAGVCNEPERMWRLFREILEGLVHVHEQGMIHRDLKPQNIFLDKNDHVKIGDFGLATAQARVTLADSSEEISKSETGDSVYSGVVGTALYVAPELMVTGKRSLYSQKVDIYSVGIIFFEMSFPGIQTKMERVKVLGNLRTSSITFPSDFDEAAMPREAEITRWCLDHVPNKRPTASELLKCRLVPTPVLEESNVLEAVREMLSNPDSKPYRLVMDEVFNQPENPTSSVIYDIEMHKKHFSETQNVAQQQTFDIISSLLRCHGAVRVNPPLLLPKCGSENDANEAAVLIGVGGNVVSLPYILRIPLARFILCKGITNIKTYAIEKVFRARKYARSRPKELWECSFDIISPSVGGLAPDAEVISVVSEIISQFPSLSSQKYEVRINHLLALESILTYCGLDESHHTEALNALASCSGLSKTQLHNCINSLPLNAQQVASLLTFIQVQGPLSRVQTSLRSITRNKHSPASSRARQALHELEALRKNLDSLNLTLPVVFDLGYSHKHHNHYNGIIFKVSAEIQKKKRTAIDVLAIGGRYDKLLTTMSVFNMYDRDGEQKLTPKHAVGVSIAVEKIVGSVMEENNLPSSTSMSGSNDPTVTSQLSHHAAVDVLVCSVGRTLLLHERLRVVRSLWEASIAAETVYDRGAMESMQEVQEKAQERGIRHLVFLSEKESGSPLVRSLTHDPLLRSTERRVPLDQLVDYLLKRLKEVESTVSMKSSSSGFSASHESSTVTMTNNSARQISIEVVPPEKLASHLRKRYESEVMTKLTSLPMMSHIQSKAQICALAVELPSPVMKSMGCALDLSHSEEEFNSSLAHHLHTVSKYKKYLSYIAERICEAKFEREMTFIILYSYKDHGYCLLI

Target ORF information:

RefSeq Version	XM_018813629.2
Organism	Ciona intestinalis(vase tunicate)
Definition	Ciona intestinalis eIF-2-alpha kinase GCN2 (LOC100187226), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018813629.2

ORF Insert Sequence:

ATGACGATGA CGGAAAGTGA ATCCTATCAA GAAAGGCAGG AAAATGAACT TGAATTGCTT 
CACTCCATAT TTGGTGAGGA GATTGAAGAT CTGAGGAAAT TGGACAAATG GAAGGTGGTC 
CGTCCTCCAG AGATCATTCT CTATCTTCAT CCACAGGAAG GGATGAGCGG AAAGAAAGAA 
ACTTTTACCA AAATTGACCT CAAAATAAAA TTTACACCAA CTTACCCAGA CAGTTTCCCC 
GAGTGTAAAC TGGAGAATGC GAAAGGTTTA TCAGAAGATG CCATAAATGA TTTGGAATCT 
GGGATTAAAA ATCTCTACAA GTCATTACAG GGTGAAGTAA TGGTGCTCGA AATTGCTCAG 
TTTGTTAAGG AGTTCCTGCA TACACACAAT GTCCCACAAT GTAACTCGGT GTACGAGGAA 
ATGGTAAAAA ACAAACAAAG ACAGCTTGAA AAAGCTGCAC AGGAAGAGGA AAAGAAAAAG 
GAAATTGAAA GAAGAAAAAA GGAATTAAAG AGGAAAGAGA TAGAAGAAGA ACTAGCAAGG 
AGGCAAAACG AAGAAAAACA AATTCGTAAA CTTATGAGGG AGGGCAGAGA TCAGAACCAA 
TCATCTCAAG ACCACACACG CCGACTTTCT GAAGATATTC CGAACGGCAA TGGCAGTCAT 
GGGAAGTCTA CAGGAGTCGT GGATTTAAAA ATCAATGGAA AACTTCCACG AATAATACAT 
AAAGGGCAAA CATTAGACTA CATTGGAAGA TCCACACAAA AACTTAAAAA GATCCAGAAG 
CATATCGAGA TCTGCCATTT AAACACCACC AGTAGTGGTA GCAATGGATC AACAGTATAC 
GCGGGCATGG ATGCAAACAC AGGTGAACTA GTTGCAGTTC ATTACTGGAA CATACCATGT 
CCTATGAGGA ATTCAATGCT CACTGACGCA GAACGAGCTA AACTCGATAA ATATATGAAA 
ACAATTAACA CAATTGAGCA GGAGTTGAAT TCTTTGGTGA AATTCTCCCA CAAACACCTA 
GTGCCCTACC TTGCAATGAG ATATAGCCAT GTTGAAAGTA GTATTGAACT TCATATTGTG 
GAGAAATATA TAACAGGTGC AAGCCTTCAA CAAAAACTCA CAAGTAGAGA CAGAGTTGGA 
ATTGACGCTG TTAGAACTTA TTCTGAGCAA CTTTTATCTG CGCTTGACTG CCTACATAAA 
AAGTCTGTTG TACATAAGAA TCTTAAGGCG AGCAGTGTTT TCTTAAGCAC CAAGGGATCC 
ATCAAGCTTG CAGATTACAG CATAGAGAAA AGAGTAATAG ACTTACATAA TGAGTCCCGT 
ATTGAACAGC CTGGTGTTCG ATTCAGTGAA GATCGAATTC CTGCTCGAAA TACAAAGAAA 
GGAGATATTT TGCGGTTGGG TATGTTGATA CTGTCACTTG CACAAGGACA AACAATACAG 
GATTATCCTC CAGAAATACC AGCGAGCTTT CCACTGGTTT TCCAAAACTT TCTTGAGAAA 
TGTCTTGTGG TTGAAGACAG GCTTCGTTAT ACAACATCTG AACTCTTGGA TCATGAATTT 
ATCAAGCCAA GTGTCACCAG TCCTATCCTT GACATCGATC TTCCTTTGGA GCAAAATCCG 
AGTCCGTTTG ATGAAGATAA GATGGATGAT GTCCTTGCAA CATCCGATGC TCCAAGACCA 
ACTCTCTCTC GTCTCCACTC GGAGTTTGAG AAGTTAGAAT GGCTTGGGAA GGGAGGGTAT 
GGGGACGTGG TGAAGGTAAA GAACATAATA GACGGACGAG ATTACGCAAT CAAACGTATT 
CTACTGAACC CACATCGATC CCTGTTTAAT AAAAAGATAA CGAGAGAAGT GAAACTTTTG 
TCTCGGTTAA ACCATGAGAA TGTTGTCAGA TATTACAACT CGTGGATTGA AACAGTTGAA 
TGTTGGGAAG ATGATGTTTC AACAAATTCA TCTAACCCAA CAACAACAAC AACAACAACA 
AATGAAACAA AATCAAACGT ATCAGATCGT ATCAATTCAC TTGGTTTTTA TGATAATATT 
GAGGTTCTAG CGCCACCTCC TGTCGCATCT AGCGTGTCAG ATTGGAGCGT TTCATTGGAG 
GAGGTGAGTC GTTGTCGTGA CGACGAGGAA GATGATGACG AAGATGTGTT CAAGACTATC 
TATGTTGACG AGGATGACTC CGAGAGCATC AAGTTTGATC ACAGTGATGA AGAAGAAGAT 
GAGATGGTGG AAGAATCAAC TAATGATGGC AAAACGTCTC GGAGTGAAGA AACCACCGTC 
ACTAGTTCCG CGTCATCAAA ATCGCTTGTG ACTCAGCGAT TTCTATACAT ACAGATGGAA 
TATTGTGAGA AGAGCACACT GCGTCAAACC ATTGATGCTG GCGTTTGTAA TGAACCTGAG 
AGAATGTGGA GATTATTCAG GGAAATTCTT GAAGGATTGG TTCACGTACA TGAGCAAGGC 
ATGATCCATA GAGATCTAAA ACCACAGAAT ATTTTCCTCG ATAAAAACGA CCATGTTAAG 
ATTGGTGATT TTGGTCTTGC TACTGCACAA GCTAGAGTGA CACTAGCTGA CAGCTCTGAA 
GAGATTTCAA AATCCGAAAC TGGAGACAGT GTATATTCTG GTGTGGTGGG AACTGCATTA 
TACGTGGCAC CTGAACTTAT GGTTACTGGT AAAAGATCAC TGTACAGTCA AAAGGTTGAC 
ATCTACAGTG TTGGAATCAT TTTCTTTGAA ATGTCATTTC CTGGTATCCA AACTAAGATG 
GAACGAGTCA AAGTTCTTGG GAACCTTAGA ACTTCTTCCA TAACCTTCCC ATCTGACTTT 
GATGAAGCTG CCATGCCTCG TGAAGCTGAG ATAACTCGAT GGTGTTTGGA TCACGTGCCA 
AACAAACGAC CAACTGCATC TGAACTTCTA AAGTGTAGAT TAGTTCCAAC TCCTGTACTA 
GAAGAATCCA ATGTACTTGA AGCAGTTCGT GAAATGTTAA GCAATCCAGA CTCAAAGCCG 
TATAGATTAG TTATGGATGA AGTTTTCAAC CAACCAGAAA ACCCAACTTC ATCTGTTATA 
TACGACATTG AAATGCATAA GAAACATTTT TCTGAGACAC AGAATGTCGC ACAACAGCAA 
ACATTTGACA TTATATCTTC CCTACTACGT TGCCATGGAG CTGTGCGGGT CAATCCTCCG 
CTCTTGTTAC CTAAATGTGG TTCAGAGAAT GATGCAAATG AAGCTGCAGT GCTAATAGGG 
GTTGGCGGTA ATGTCGTATC ACTGCCTTAT ATTCTAAGGA TTCCACTGGC TCGTTTTATT 
CTGTGTAAAG GAATCACAAA CATTAAAACT TACGCCATTG AAAAAGTTTT CCGAGCAAGA 
AAATATGCGA GGTCCCGACC TAAGGAATTA TGGGAATGTT CGTTTGACAT CATTTCCCCC 
TCAGTGGGAG GTCTCGCTCC CGACGCGGAA GTGATCTCTG TTGTTTCCGA GATCATCTCC 
CAATTCCCAT CTCTCTCCTC CCAGAAGTAC GAGGTTCGGA TCAACCACTT ATTGGCGCTA 
GAGTCAATAC TAACATACTG TGGTCTCGAT GAAAGCCACC ACACGGAGGC GCTCAATGCA 
CTCGCTTCAT GCAGTGGCCT CTCTAAAACA CAGCTCCACA ACTGCATCAA CAGCTTGCCT 
CTCAATGCTC AACAGGTTGC TTCCCTTCTT ACATTCATCC AAGTCCAAGG CCCGCTGTCC 
CGGGTTCAAA CCTCGCTGCG GTCGATTACC CGGAACAAGC ACTCACCCGC GTCGTCCAGA 
GCACGACAGG CGCTCCACGA ACTCGAAGCT CTTCGGAAAA ATCTTGATTC TTTAAACTTG 
ACACTACCTG TTGTGTTTGA TCTTGGTTAT TCTCATAAAC ACCACAACCA TTATAATGGC 
ATCATATTTA AAGTTTCAGC GGAAATCCAA AAGAAAAAAA GGACAGCAAT TGATGTGTTG 
GCCATCGGTG GTCGATATGA TAAACTGCTC ACAACCATGT CTGTATTCAA CATGTATGAC 
AGAGATGGGG AACAGAAGTT AACACCTAAG CATGCTGTGG GTGTTAGTAT TGCTGTGGAG 
AAAATTGTAG GATCCGTAAT GGAAGAAAAC AATCTTCCCA GCTCAACAAG TATGAGTGGC 
AGCAACGACC CCACTGTGAC ATCACAATTA TCCCACCACG CTGCAGTTGA CGTGTTGGTT 
TGTTCTGTCG GGCGAACTCT TCTGTTGCAC GAACGATTGA GGGTTGTAAG ATCTCTATGG 
GAGGCCTCGA TAGCAGCAGA AACCGTTTAT GATCGCGGAG CAATGGAATC AATGCAAGAA 
GTGCAGGAGA AAGCTCAAGA AAGGGGGATC CGCCATCTTG TATTTTTAAG CGAGAAAGAA 
AGTGGCTCCC CGTTAGTACG ATCATTGACG CACGACCCGC TGTTACGCTC TACTGAACGC 
AGAGTACCGC TGGATCAGTT GGTGGATTAT TTGTTAAAAC GATTGAAAGA AGTTGAATCA 
ACTGTATCAA TGAAATCTTC CTCATCGGGG TTCTCTGCAT CACACGAGTC CTCCACTGTT 
ACCATGACGA ACAATAGTGC TCGTCAAATC AGCATCGAGG TTGTTCCTCC CGAGAAACTT 
GCCTCACATT TAAGGAAGAG ATATGAGTCT GAAGTTATGA CCAAACTTAC AAGTCTACCT 
ATGATGTCCC ACATTCAATC TAAAGCTCAA ATTTGTGCCC TTGCGGTTGA ACTGCCATCA 
CCAGTTATGA AGAGCATGGG ATGTGCTCTT GATCTTTCTC ACTCTGAGGA AGAGTTTAAC 
TCTTCTCTTG CCCATCATCT ACACACTGTA TCCAAGTATA AAAAATACTT GTCCTATATC 
GCTGAGAGAA TTTGCGAGGC AAAATTCGAA CGAGAAATGA CTTTCATCAT TCTTTACAGC 
TACAAAGACC ATGGCTACTG TCTTTTGATA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100187226 ( XM_018813629.1 ) cDNA ORF clone, Ciona intestinalis(vase tunicate) -> XP_018669174.1 Ciona intestinalis eIF-2-alpha kinase GCN2 (LOC100187226), transcript variant X1, mRNA.

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CloneID	OCi70880
Clone ID Related Accession (Same CDS sequence)	XM_018813629.2 , XM_018813629.1
Accession Version	XM_018813629.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-20
Organism	Ciona intestinalis(vase tunicate)
Product	eIF-2-alpha kinase GCN2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_020175.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ciona intestinalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGATGA CGGAAAGTGA ATCCTATCAA GAAAGGCAGG AAAATGAACT TGAATTGCTT 
CACTCCATAT TTGGTGAGGA GATTGAAGAT CTGAGGAAAT TGGACAAATG GAAGGTGGTC 
CGTCCTCCAG AGATCATTCT CTATCTTCAT CCACAGGAAG GGATGAGCGG AAAGAAAGAA 
ACTTTTACCA AAATTGACCT CAAAATAAAA TTTACACCAA CTTACCCAGA CAGTTTCCCC 
GAGTGTAAAC TGGAGAATGC GAAAGGTTTA TCAGAAGATG CCATAAATGA TTTGGAATCT 
GGGATTAAAA ATCTCTACAA GTCATTACAG GGTGAAGTAA TGGTGCTCGA AATTGCTCAG 
TTTGTTAAGG AGTTCCTGCA TACACACAAT GTCCCACAAT GTAACTCGGT GTACGAGGAA 
ATGGTAAAAA ACAAACAAAG ACAGCTTGAA AAAGCTGCAC AGGAAGAGGA AAAGAAAAAG 
GAAATTGAAA GAAGAAAAAA GGAATTAAAG AGGAAAGAGA TAGAAGAAGA ACTAGCAAGG 
AGGCAAAACG AAGAAAAACA AATTCGTAAA CTTATGAGGG AGGGCAGAGA TCAGAACCAA 
TCATCTCAAG ACCACACACG CCGACTTTCT GAAGATATTC CGAACGGCAA TGGCAGTCAT 
GGGAAGTCTA CAGGAGTCGT GGATTTAAAA ATCAATGGAA AACTTCCACG AATAATACAT 
AAAGGGCAAA CATTAGACTA CATTGGAAGA TCCACACAAA AACTTAAAAA GATCCAGAAG 
CATATCGAGA TCTGCCATTT AAACACCACC AGTAGTGGTA GCAATGGATC AACAGTATAC 
GCGGGCATGG ATGCAAACAC AGGTGAACTA GTTGCAGTTC ATTACTGGAA CATACCATGT 
CCTATGAGGA ATTCAATGCT CACTGACGCA GAACGAGCTA AACTCGATAA ATATATGAAA 
ACAATTAACA CAATTGAGCA GGAGTTGAAT TCTTTGGTGA AATTCTCCCA CAAACACCTA 
GTGCCCTACC TTGCAATGAG ATATAGCCAT GTTGAAAGTA GTATTGAACT TCATATTGTG 
GAGAAATATA TAACAGGTGC AAGCCTTCAA CAAAAACTCA CAAGTAGAGA CAGAGTTGGA 
ATTGACGCTG TTAGAACTTA TTCTGAGCAA CTTTTATCTG CGCTTGACTG CCTACATAAA 
AAGTCTGTTG TACATAAGAA TCTTAAGGCG AGCAGTGTTT TCTTAAGCAC CAAGGGATCC 
ATCAAGCTTG CAGATTACAG CATAGAGAAA AGAGTAATAG ACTTACATAA TGAGTCCCGT 
ATTGAACAGC CTGGTGTTCG ATTCAGTGAA GATCGAATTC CTGCTCGAAA TACAAAGAAA 
GGAGATATTT TGCGGTTGGG TATGTTGATA CTGTCACTTG CACAAGGACA AACAATACAG 
GATTATCCTC CAGAAATACC AGCGAGCTTT CCACTGGTTT TCCAAAACTT TCTTGAGAAA 
TGTCTTGTGG TTGAAGACAG GCTTCGTTAT ACAACATCTG AACTCTTGGA TCATGAATTT 
ATCAAGCCAA GTGTCACCAG TCCTATCCTT GACATCGATC TTCCTTTGGA GCAAAATCCG 
AGTCCGTTTG ATGAAGATAA GATGGATGAT GTCCTTGCAA CATCCGATGC TCCAAGACCA 
ACTCTCTCTC GTCTCCACTC GGAGTTTGAG AAGTTAGAAT GGCTTGGGAA GGGAGGGTAT 
GGGGACGTGG TGAAGGTAAA GAACATAATA GACGGACGAG ATTACGCAAT CAAACGTATT 
CTACTGAACC CACATCGATC CCTGTTTAAT AAAAAGATAA CGAGAGAAGT GAAACTTTTG 
TCTCGGTTAA ACCATGAGAA TGTTGTCAGA TATTACAACT CGTGGATTGA AACAGTTGAA 
TGTTGGGAAG ATGATGTTTC AACAAATTCA TCTAACCCAA CAACAACAAC AACAACAACA 
AATGAAACAA AATCAAACGT ATCAGATCGT ATCAATTCAC TTGGTTTTTA TGATAATATT 
GAGGTTCTAG CGCCACCTCC TGTCGCATCT AGCGTGTCAG ATTGGAGCGT TTCATTGGAG 
GAGGTGAGTC GTTGTCGTGA CGACGAGGAA GATGATGACG AAGATGTGTT CAAGACTATC 
TATGTTGACG AGGATGACTC CGAGAGCATC AAGTTTGATC ACAGTGATGA AGAAGAAGAT 
GAGATGGTGG AAGAATCAAC TAATGATGGC AAAACGTCTC GGAGTGAAGA AACCACCGTC 
ACTAGTTCCG CGTCATCAAA ATCGCTTGTG ACTCAGCGAT TTCTATACAT ACAGATGGAA 
TATTGTGAGA AGAGCACACT GCGTCAAACC ATTGATGCTG GCGTTTGTAA TGAACCTGAG 
AGAATGTGGA GATTATTCAG GGAAATTCTT GAAGGATTGG TTCACGTACA TGAGCAAGGC 
ATGATCCATA GAGATCTAAA ACCACAGAAT ATTTTCCTCG ATAAAAACGA CCATGTTAAG 
ATTGGTGATT TTGGTCTTGC TACTGCACAA GCTAGAGTGA CACTAGCTGA CAGCTCTGAA 
GAGATTTCAA AATCCGAAAC TGGAGACAGT GTATATTCTG GTGTGGTGGG AACTGCATTA 
TACGTGGCAC CTGAACTTAT GGTTACTGGT AAAAGATCAC TGTACAGTCA AAAGGTTGAC 
ATCTACAGTG TTGGAATCAT TTTCTTTGAA ATGTCATTTC CTGGTATCCA AACTAAGATG 
GAACGAGTCA AAGTTCTTGG GAACCTTAGA ACTTCTTCCA TAACCTTCCC ATCTGACTTT 
GATGAAGCTG CCATGCCTCG TGAAGCTGAG ATAACTCGAT GGTGTTTGGA TCACGTGCCA 
AACAAACGAC CAACTGCATC TGAACTTCTA AAGTGTAGAT TAGTTCCAAC TCCTGTACTA 
GAAGAATCCA ATGTACTTGA AGCAGTTCGT GAAATGTTAA GCAATCCAGA CTCAAAGCCG 
TATAGATTAG TTATGGATGA AGTTTTCAAC CAACCAGAAA ACCCAACTTC ATCTGTTATA 
TACGACATTG AAATGCATAA GAAACATTTT TCTGAGACAC AGAATGTCGC ACAACAGCAA 
ACATTTGACA TTATATCTTC CCTACTACGT TGCCATGGAG CTGTGCGGGT CAATCCTCCG 
CTCTTGTTAC CTAAATGTGG TTCAGAGAAT GATGCAAATG AAGCTGCAGT GCTAATAGGG 
GTTGGCGGTA ATGTCGTATC ACTGCCTTAT ATTCTAAGGA TTCCACTGGC TCGTTTTATT 
CTGTGTAAAG GAATCACAAA CATTAAAACT TACGCCATTG AAAAAGTTTT CCGAGCAAGA 
AAATATGCGA GGTCCCGACC TAAGGAATTA TGGGAATGTT CGTTTGACAT CATTTCCCCC 
TCAGTGGGAG GTCTCGCTCC CGACGCGGAA GTGATCTCTG TTGTTTCCGA GATCATCTCC 
CAATTCCCAT CTCTCTCCTC CCAGAAGTAC GAGGTTCGGA TCAACCACTT ATTGGCGCTA 
GAGTCAATAC TAACATACTG TGGTCTCGAT GAAAGCCACC ACACGGAGGC GCTCAATGCA 
CTCGCTTCAT GCAGTGGCCT CTCTAAAACA CAGCTCCACA ACTGCATCAA CAGCTTGCCT 
CTCAATGCTC AACAGGTTGC TTCCCTTCTT ACATTCATCC AAGTCCAAGG CCCGCTGTCC 
CGGGTTCAAA CCTCGCTGCG GTCGATTACC CGGAACAAGC ACTCACCCGC GTCGTCCAGA 
GCACGACAGG CGCTCCACGA ACTCGAAGCT CTTCGGAAAA ATCTTGATTC TTTAAACTTG 
ACACTACCTG TTGTGTTTGA TCTTGGTTAT TCTCATAAAC ACCACAACCA TTATAATGGC 
ATCATATTTA AAGTTTCAGC GGAAATCCAA AAGAAAAAAA GGACAGCAAT TGATGTGTTG 
GCCATCGGTG GTCGATATGA TAAACTGCTC ACAACCATGT CTGTATTCAA CATGTATGAC 
AGAGATGGGG AACAGAAGTT AACACCTAAG CATGCTGTGG GTGTTAGTAT TGCTGTGGAG 
AAAATTGTAG GATCCGTAAT GGAAGAAAAC AATCTTCCCA GCTCAACAAG TATGAGTGGC 
AGCAACGACC CCACTGTGAC ATCACAATTA TCCCACCACG CTGCAGTTGA CGTGTTGGTT 
TGTTCTGTCG GGCGAACTCT TCTGTTGCAC GAACGATTGA GGGTTGTAAG ATCTCTATGG 
GAGGCCTCGA TAGCAGCAGA AACCGTTTAT GATCGCGGAG CAATGGAATC AATGCAAGAA 
GTGCAGGAGA AAGCTCAAGA AAGGGGGATC CGCCATCTTG TATTTTTAAG CGAGAAAGAA 
AGTGGCTCCC CGTTAGTACG ATCATTGACG CACGACCCGC TGTTACGCTC TACTGAACGC 
AGAGTACCGC TGGATCAGTT GGTGGATTAT TTGTTAAAAC GATTGAAAGA AGTTGAATCA 
ACTGTATCAA TGAAATCTTC CTCATCGGGG TTCTCTGCAT CACACGAGTC CTCCACTGTT 
ACCATGACGA ACAATAGTGC TCGTCAAATC AGCATCGAGG TTGTTCCTCC CGAGAAACTT 
GCCTCACATT TAAGGAAGAG ATATGAGTCT GAAGTTATGA CCAAACTTAC AAGTCTACCT 
ATGATGTCCC ACATTCAATC TAAAGCTCAA ATTTGTGCCC TTGCGGTTGA ACTGCCATCA 
CCAGTTATGA AGAGCATGGG ATGTGCTCTT GATCTTTCTC ACTCTGAGGA AGAGTTTAAC 
TCTTCTCTTG CCCATCATCT ACACACTGTA TCCAAGTATA AAAAATACTT GTCCTATATC 
GCTGAGAGAA TTTGCGAGGC AAAATTCGAA CGAGAAATGA CTTTCATCAT TCTTTACAGC 
TACAAAGACC ATGGCTACTG TCTTTTGATA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018669174.1
CDS	107..5059
Translation	MTMTESESYQERQENELELLHSIFGEEIEDLRKLDKWKVVRPPEIILYLHPQEGMSGKKETFTKIDLKIKFTPTYPDSFPECKLENAKGLSEDAINDLESGIKNLYKSLQGEVMVLEIAQFVKEFLHTHNVPQCNSVYEEMVKNKQRQLEKAAQEEEKKKEIERRKKELKRKEIEEELARRQNEEKQIRKLMREGRDQNQSSQDHTRRLSEDIPNGNGSHGKSTGVVDLKINGKLPRIIHKGQTLDYIGRSTQKLKKIQKHIEICHLNTTSSGSNGSTVYAGMDANTGELVAVHYWNIPCPMRNSMLTDAERAKLDKYMKTINTIEQELNSLVKFSHKHLVPYLAMRYSHVESSIELHIVEKYITGASLQQKLTSRDRVGIDAVRTYSEQLLSALDCLHKKSVVHKNLKASSVFLSTKGSIKLADYSIEKRVIDLHNESRIEQPGVRFSEDRIPARNTKKGDILRLGMLILSLAQGQTIQDYPPEIPASFPLVFQNFLEKCLVVEDRLRYTTSELLDHEFIKPSVTSPILDIDLPLEQNPSPFDEDKMDDVLATSDAPRPTLSRLHSEFEKLEWLGKGGYGDVVKVKNIIDGRDYAIKRILLNPHRSLFNKKITREVKLLSRLNHENVVRYYNSWIETVECWEDDVSTNSSNPTTTTTTTNETKSNVSDRINSLGFYDNIEVLAPPPVASSVSDWSVSLEEVSRCRDDEEDDDEDVFKTIYVDEDDSESIKFDHSDEEEDEMVEESTNDGKTSRSEETTVTSSASSKSLVTQRFLYIQMEYCEKSTLRQTIDAGVCNEPERMWRLFREILEGLVHVHEQGMIHRDLKPQNIFLDKNDHVKIGDFGLATAQARVTLADSSEEISKSETGDSVYSGVVGTALYVAPELMVTGKRSLYSQKVDIYSVGIIFFEMSFPGIQTKMERVKVLGNLRTSSITFPSDFDEAAMPREAEITRWCLDHVPNKRPTASELLKCRLVPTPVLEESNVLEAVREMLSNPDSKPYRLVMDEVFNQPENPTSSVIYDIEMHKKHFSETQNVAQQQTFDIISSLLRCHGAVRVNPPLLLPKCGSENDANEAAVLIGVGGNVVSLPYILRIPLARFILCKGITNIKTYAIEKVFRARKYARSRPKELWECSFDIISPSVGGLAPDAEVISVVSEIISQFPSLSSQKYEVRINHLLALESILTYCGLDESHHTEALNALASCSGLSKTQLHNCINSLPLNAQQVASLLTFIQVQGPLSRVQTSLRSITRNKHSPASSRARQALHELEALRKNLDSLNLTLPVVFDLGYSHKHHNHYNGIIFKVSAEIQKKKRTAIDVLAIGGRYDKLLTTMSVFNMYDRDGEQKLTPKHAVGVSIAVEKIVGSVMEENNLPSSTSMSGSNDPTVTSQLSHHAAVDVLVCSVGRTLLLHERLRVVRSLWEASIAAETVYDRGAMESMQEVQEKAQERGIRHLVFLSEKESGSPLVRSLTHDPLLRSTERRVPLDQLVDYLLKRLKEVESTVSMKSSSSGFSASHESSTVTMTNNSARQISIEVVPPEKLASHLRKRYESEVMTKLTSLPMMSHIQSKAQICALAVELPSPVMKSMGCALDLSHSEEEFNSSLAHHLHTVSKYKKYLSYIAERICEAKFEREMTFIILYSYKDHGYCLLI

Target ORF information:

RefSeq Version	XM_018813629.1
Organism	Ciona intestinalis(vase tunicate)
Definition	Ciona intestinalis eIF-2-alpha kinase GCN2 (LOC100187226), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018813629.1

ORF Insert Sequence:

ATGACGATGA CGGAAAGTGA ATCCTATCAA GAAAGGCAGG AAAATGAACT TGAATTGCTT 
CACTCCATAT TTGGTGAGGA GATTGAAGAT CTGAGGAAAT TGGACAAATG GAAGGTGGTC 
CGTCCTCCAG AGATCATTCT CTATCTTCAT CCACAGGAAG GGATGAGCGG AAAGAAAGAA 
ACTTTTACCA AAATTGACCT CAAAATAAAA TTTACACCAA CTTACCCAGA CAGTTTCCCC 
GAGTGTAAAC TGGAGAATGC GAAAGGTTTA TCAGAAGATG CCATAAATGA TTTGGAATCT 
GGGATTAAAA ATCTCTACAA GTCATTACAG GGTGAAGTAA TGGTGCTCGA AATTGCTCAG 
TTTGTTAAGG AGTTCCTGCA TACACACAAT GTCCCACAAT GTAACTCGGT GTACGAGGAA 
ATGGTAAAAA ACAAACAAAG ACAGCTTGAA AAAGCTGCAC AGGAAGAGGA AAAGAAAAAG 
GAAATTGAAA GAAGAAAAAA GGAATTAAAG AGGAAAGAGA TAGAAGAAGA ACTAGCAAGG 
AGGCAAAACG AAGAAAAACA AATTCGTAAA CTTATGAGGG AGGGCAGAGA TCAGAACCAA 
TCATCTCAAG ACCACACACG CCGACTTTCT GAAGATATTC CGAACGGCAA TGGCAGTCAT 
GGGAAGTCTA CAGGAGTCGT GGATTTAAAA ATCAATGGAA AACTTCCACG AATAATACAT 
AAAGGGCAAA CATTAGACTA CATTGGAAGA TCCACACAAA AACTTAAAAA GATCCAGAAG 
CATATCGAGA TCTGCCATTT AAACACCACC AGTAGTGGTA GCAATGGATC AACAGTATAC 
GCGGGCATGG ATGCAAACAC AGGTGAACTA GTTGCAGTTC ATTACTGGAA CATACCATGT 
CCTATGAGGA ATTCAATGCT CACTGACGCA GAACGAGCTA AACTCGATAA ATATATGAAA 
ACAATTAACA CAATTGAGCA GGAGTTGAAT TCTTTGGTGA AATTCTCCCA CAAACACCTA 
GTGCCCTACC TTGCAATGAG ATATAGCCAT GTTGAAAGTA GTATTGAACT TCATATTGTG 
GAGAAATATA TAACAGGTGC AAGCCTTCAA CAAAAACTCA CAAGTAGAGA CAGAGTTGGA 
ATTGACGCTG TTAGAACTTA TTCTGAGCAA CTTTTATCTG CGCTTGACTG CCTACATAAA 
AAGTCTGTTG TACATAAGAA TCTTAAGGCG AGCAGTGTTT TCTTAAGCAC CAAGGGATCC 
ATCAAGCTTG CAGATTACAG CATAGAGAAA AGAGTAATAG ACTTACATAA TGAGTCCCGT 
ATTGAACAGC CTGGTGTTCG ATTCAGTGAA GATCGAATTC CTGCTCGAAA TACAAAGAAA 
GGAGATATTT TGCGGTTGGG TATGTTGATA CTGTCACTTG CACAAGGACA AACAATACAG 
GATTATCCTC CAGAAATACC AGCGAGCTTT CCACTGGTTT TCCAAAACTT TCTTGAGAAA 
TGTCTTGTGG TTGAAGACAG GCTTCGTTAT ACAACATCTG AACTCTTGGA TCATGAATTT 
ATCAAGCCAA GTGTCACCAG TCCTATCCTT GACATCGATC TTCCTTTGGA GCAAAATCCG 
AGTCCGTTTG ATGAAGATAA GATGGATGAT GTCCTTGCAA CATCCGATGC TCCAAGACCA 
ACTCTCTCTC GTCTCCACTC GGAGTTTGAG AAGTTAGAAT GGCTTGGGAA GGGAGGGTAT 
GGGGACGTGG TGAAGGTAAA GAACATAATA GACGGACGAG ATTACGCAAT CAAACGTATT 
CTACTGAACC CACATCGATC CCTGTTTAAT AAAAAGATAA CGAGAGAAGT GAAACTTTTG 
TCTCGGTTAA ACCATGAGAA TGTTGTCAGA TATTACAACT CGTGGATTGA AACAGTTGAA 
TGTTGGGAAG ATGATGTTTC AACAAATTCA TCTAACCCAA CAACAACAAC AACAACAACA 
AATGAAACAA AATCAAACGT ATCAGATCGT ATCAATTCAC TTGGTTTTTA TGATAATATT 
GAGGTTCTAG CGCCACCTCC TGTCGCATCT AGCGTGTCAG ATTGGAGCGT TTCATTGGAG 
GAGGTGAGTC GTTGTCGTGA CGACGAGGAA GATGATGACG AAGATGTGTT CAAGACTATC 
TATGTTGACG AGGATGACTC CGAGAGCATC AAGTTTGATC ACAGTGATGA AGAAGAAGAT 
GAGATGGTGG AAGAATCAAC TAATGATGGC AAAACGTCTC GGAGTGAAGA AACCACCGTC 
ACTAGTTCCG CGTCATCAAA ATCGCTTGTG ACTCAGCGAT TTCTATACAT ACAGATGGAA 
TATTGTGAGA AGAGCACACT GCGTCAAACC ATTGATGCTG GCGTTTGTAA TGAACCTGAG 
AGAATGTGGA GATTATTCAG GGAAATTCTT GAAGGATTGG TTCACGTACA TGAGCAAGGC 
ATGATCCATA GAGATCTAAA ACCACAGAAT ATTTTCCTCG ATAAAAACGA CCATGTTAAG 
ATTGGTGATT TTGGTCTTGC TACTGCACAA GCTAGAGTGA CACTAGCTGA CAGCTCTGAA 
GAGATTTCAA AATCCGAAAC TGGAGACAGT GTATATTCTG GTGTGGTGGG AACTGCATTA 
TACGTGGCAC CTGAACTTAT GGTTACTGGT AAAAGATCAC TGTACAGTCA AAAGGTTGAC 
ATCTACAGTG TTGGAATCAT TTTCTTTGAA ATGTCATTTC CTGGTATCCA AACTAAGATG 
GAACGAGTCA AAGTTCTTGG GAACCTTAGA ACTTCTTCCA TAACCTTCCC ATCTGACTTT 
GATGAAGCTG CCATGCCTCG TGAAGCTGAG ATAACTCGAT GGTGTTTGGA TCACGTGCCA 
AACAAACGAC CAACTGCATC TGAACTTCTA AAGTGTAGAT TAGTTCCAAC TCCTGTACTA 
GAAGAATCCA ATGTACTTGA AGCAGTTCGT GAAATGTTAA GCAATCCAGA CTCAAAGCCG 
TATAGATTAG TTATGGATGA AGTTTTCAAC CAACCAGAAA ACCCAACTTC ATCTGTTATA 
TACGACATTG AAATGCATAA GAAACATTTT TCTGAGACAC AGAATGTCGC ACAACAGCAA 
ACATTTGACA TTATATCTTC CCTACTACGT TGCCATGGAG CTGTGCGGGT CAATCCTCCG 
CTCTTGTTAC CTAAATGTGG TTCAGAGAAT GATGCAAATG AAGCTGCAGT GCTAATAGGG 
GTTGGCGGTA ATGTCGTATC ACTGCCTTAT ATTCTAAGGA TTCCACTGGC TCGTTTTATT 
CTGTGTAAAG GAATCACAAA CATTAAAACT TACGCCATTG AAAAAGTTTT CCGAGCAAGA 
AAATATGCGA GGTCCCGACC TAAGGAATTA TGGGAATGTT CGTTTGACAT CATTTCCCCC 
TCAGTGGGAG GTCTCGCTCC CGACGCGGAA GTGATCTCTG TTGTTTCCGA GATCATCTCC 
CAATTCCCAT CTCTCTCCTC CCAGAAGTAC GAGGTTCGGA TCAACCACTT ATTGGCGCTA 
GAGTCAATAC TAACATACTG TGGTCTCGAT GAAAGCCACC ACACGGAGGC GCTCAATGCA 
CTCGCTTCAT GCAGTGGCCT CTCTAAAACA CAGCTCCACA ACTGCATCAA CAGCTTGCCT 
CTCAATGCTC AACAGGTTGC TTCCCTTCTT ACATTCATCC AAGTCCAAGG CCCGCTGTCC 
CGGGTTCAAA CCTCGCTGCG GTCGATTACC CGGAACAAGC ACTCACCCGC GTCGTCCAGA 
GCACGACAGG CGCTCCACGA ACTCGAAGCT CTTCGGAAAA ATCTTGATTC TTTAAACTTG 
ACACTACCTG TTGTGTTTGA TCTTGGTTAT TCTCATAAAC ACCACAACCA TTATAATGGC 
ATCATATTTA AAGTTTCAGC GGAAATCCAA AAGAAAAAAA GGACAGCAAT TGATGTGTTG 
GCCATCGGTG GTCGATATGA TAAACTGCTC ACAACCATGT CTGTATTCAA CATGTATGAC 
AGAGATGGGG AACAGAAGTT AACACCTAAG CATGCTGTGG GTGTTAGTAT TGCTGTGGAG 
AAAATTGTAG GATCCGTAAT GGAAGAAAAC AATCTTCCCA GCTCAACAAG TATGAGTGGC 
AGCAACGACC CCACTGTGAC ATCACAATTA TCCCACCACG CTGCAGTTGA CGTGTTGGTT 
TGTTCTGTCG GGCGAACTCT TCTGTTGCAC GAACGATTGA GGGTTGTAAG ATCTCTATGG 
GAGGCCTCGA TAGCAGCAGA AACCGTTTAT GATCGCGGAG CAATGGAATC AATGCAAGAA 
GTGCAGGAGA AAGCTCAAGA AAGGGGGATC CGCCATCTTG TATTTTTAAG CGAGAAAGAA 
AGTGGCTCCC CGTTAGTACG ATCATTGACG CACGACCCGC TGTTACGCTC TACTGAACGC 
AGAGTACCGC TGGATCAGTT GGTGGATTAT TTGTTAAAAC GATTGAAAGA AGTTGAATCA 
ACTGTATCAA TGAAATCTTC CTCATCGGGG TTCTCTGCAT CACACGAGTC CTCCACTGTT 
ACCATGACGA ACAATAGTGC TCGTCAAATC AGCATCGAGG TTGTTCCTCC CGAGAAACTT 
GCCTCACATT TAAGGAAGAG ATATGAGTCT GAAGTTATGA CCAAACTTAC AAGTCTACCT 
ATGATGTCCC ACATTCAATC TAAAGCTCAA ATTTGTGCCC TTGCGGTTGA ACTGCCATCA 
CCAGTTATGA AGAGCATGGG ATGTGCTCTT GATCTTTCTC ACTCTGAGGA AGAGTTTAAC 
TCTTCTCTTG CCCATCATCT ACACACTGTA TCCAAGTATA AAAAATACTT GTCCTATATC 
GCTGAGAGAA TTTGCGAGGC AAAATTCGAA CGAGAAATGA CTTTCATCAT TCTTTACAGC 
TACAAAGACC ATGGCTACTG TCTTTTGATA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100187226 ( XM_018813630.1 ) cDNA ORF clone, Ciona intestinalis(vase tunicate) -> XP_018669175.1 Ciona intestinalis eIF-2-alpha kinase GCN2 (LOC100187226), transcript variant X2, mRNA.

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CloneID	OCi70881
Clone ID Related Accession (Same CDS sequence)	XM_018813630.1
Accession Version	XM_018813630.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-20
Organism	Ciona intestinalis(vase tunicate)
Product	eIF-2-alpha kinase GCN2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_020175.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ciona intestinalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGATGA CGGAAAGTGA ATCCTATCAA GAAAGGCAGG AAAATGAACT TGAATTGCTT 
CACTCCATAT TTGGTGAGGA GATTGAAGAT CTGAGGAAAT TGGACAAATG GAAGGTGGTC 
CGTCCTCCAG AGATCATTCT CTATCTTCAT CCACAGGAAG GGATGAGCGG AAAGAAAGAA 
ACTTTTACCA AAATTGACCT CAAAATAAAA TTTACACCAA CTTACCCAGA CAGTTTCCCC 
GAGTGTAAAC TGGAGAATGC GAAAGGTTTA TCAGAAGATG CCATAAATGA TTTGGAATCT 
GGGATTAAAA ATCTCTACAA GTCATTACAG GGTGAAGTAA TGGTGCTCGA AATTGCTCAG 
TTTGTTAAGG AGTTCCTGCA TACACACAAT GTCCCACAAT GTAACTCGGT GTACGAGGAA 
ATGGTAAAAA ACAAACAAAG ACAGCTTGAA AAAGCTGCAC AGGAAGAGGA AAAGAAAAAG 
GAAATTGAAA GAAGAAAAAA GGAATTAAAG AGGAAAGAGA TAGAAGAAGA ACTAGCAAGG 
AGGCAAAACG AAGAAAAACA AATTCGTAAA CTTATGAGGG AGGGCAGAGA TCAGAACCAA 
TCATCTCAAG ACCACACACG CCGACTTTCT GAAGATATTC CGAACGGCAA TGGCAGTCAT 
GGGAAGTCTA CAGGAGTCGT GGATTTAAAA ATCAATGGAA AACTTCCACG AATAATACAT 
AAAGGGCAAA CATTAGACTA CATTGGAAGA TCCACACAAA AACTTAAAAA GATCCAGAAG 
CATATCGAGA TCTGCCATTT AAACACCACC AGTAGTGGTA GCAATGGATC AACAGTATAC 
GCGGGCATGG ATGCAAACAC AGGTGAACTA GTTGCAGTTC ATTACTGGAA CATACCATGT 
CCTATGAGGA ATTCAATGCT CACTGACGCA GAACGAGCTA AACTCGATAA ATATATGAAA 
ACAATTAACA CAATTGAGCA GGAGTTGAAT TCTTTGGTGA AATTCTCCCA CAAACACCTA 
GTGCCCTACC TTGCAATGAG ATATAGCCAT GTTGAAAGTA GTATTGAACT TCATATTGTG 
GAGAAATATA TAACAGGTGC AAGCCTTCAA CAAAAACTCA CAAGTAGAGA CAGAGTTGGA 
ATTGACGCTG TTAGAACTTA TTCTGAGCAA CTTTTATCTG CGCTTGACTG CCTACATAAA 
AAGTCTGTTG TACATAAGAA TCTTAAGGCG AGCAGTGTTT TCTTAAGCAC CAAGGGATCC 
ATCAAGCTTG CAGATTACAG CATAGAGAAA AGAGTAATAG ACTTACATAA TGAGTCCCGT 
ATTGAACAGC CTGGTGTTCG ATTCAGTGAA GATCGAATTC CTGCTCGAAA TACAAAGAAA 
GGAGATATTT TGCGGTTGGG TATGTTGATA CTGTCACTTG CACAAGGACA AACAATACAG 
GATTATCCTC CAGAAATACC AGCGAGCTTT CCACTGGTTT TCCAAAACTT TCTTGAGAAA 
TGTCTTGTGG TTGAAGACAG GCTTCGTTAT ACAACATCTG AACTCTTGGA TCATGAATTT 
ATCAAGCCAA GTGTCACCAG TCCTATCCTT GACATCGATC TTCCTTTGGA GCAAAATCCG 
AGTCCGTTTG ATGAAGATAA GATGGATGAT GTCCTTGCAA CATCCGATGC TCCAAGACCA 
ACTCTCTCTC GTCTCCACTC GGAGTTTGAG AAGTTAGAAT GGCTTGGGAA GGGAGGGTAT 
GGGGACGTGG TGAAGGTAAA GAACATAATA GACGGACGAG ATTACGCAAT CAAACGTATT 
CTACTGAACC CACATCGATC CCTGTTTAAT AAAAAGATAA CGAGAGAAGT GAAACTTTTG 
TCTCGGTTAA ACCATGAGAA TGTTGTCAGA TATTACAACT CGTGGATTGA AACAGTTGAA 
TGTTGGGAAG ATGATGTTTC AACAAATTCA TCTAACCCAA CAACAACAAC AACAACAACA 
AATGAAACAA AATCAAACGT ATCAGATCGT ATCAATTCAC TTGGTTTTTA TGATAATATT 
GAGGTTCTAG CGCCACCTCC TGTCGCATCT AGCGTGTCAG ATTGGAGCGT TTCATTGGAG 
GAGGTGAGTC GTTGTCGTGA CGACGAGGAA GATGATGACG AAGATGTGTT CAAGACTATC 
TATGTTGACG AGGATGACTC CGAGAGCATC AAGTTTGATC ACAGTGATGA AGAAGAAGAT 
GAGATGGTGG AAGAATCAAC TAATGATGGC AAAACGTCTC GGAGTGAAGA AACCACCGTC 
ACTAGTTCCG CGTCATCAAA ATCGCTTGTG ACTCAGCGAT TTCTATACAT ACAGATGGAA 
TATTGTGAGA AGAGCACACT GCGTCAAACC ATTGATGCTG GCGTTTGTAA TGAACCTGAG 
AGAATGTGGA GATTATTCAG GGAAATTCTT GAAGGATTGG TTCACGTACA TGAGCAAGGC 
ATGATCCATA GAGATCTAAA ACCACAGAAT ATTTTCCTCG ATAAAAACGA CCATGTTAAG 
ATTGGTGATT TTGGTCTTGC TACTGCACAA GCTAGAGTGA CACTAGCTGA CAGCTCTGAA 
GAGATTTCAA AATCCGAAAC TGGAGACAGT GTATATTCTG GTGTGGTGGG AACTGCATTA 
TACGTGGCAC CTGAACTTAT GGTTACTGGT AAAAGATCAC TGTACAGTCA AAAGGTTGAC 
ATCTACAGTG TTGGAATCAT TTTCTTTGAA ATGTCATTTC CTGGTATCCA AACTAAGATG 
GAACGAGTCA AAGTTCTTGG GAACCTTAGA ACTTCTTCCA TAACCTTCCC ATCTGACTTT 
GATGAAGCTG CCATGCCTCG TGAAGCTGAG ATAACTCGAT GGTGTTTGGA TCACGTGCCA 
AACAAACGAC CAACTGCATC TGAACTTCTA AAGTGTAGAT TAGTTCCAAC TCCTGTACTA 
GAAGAATCCA ATGTACTTGA AGCAGTTCGT GAAATGTTAA GCAATCCAGA CTCAAAGCCG 
TATAGATTAG TTATGGATGA AGTTTTCAAC CAACCAGAAA ACCCAACTTC ATCTGTTATA 
TACGACATTG AAATGCATAA GAAACATTTT TCTGAGACAC AGAATGTCGC ACAACAGCAA 
ACATTTGACA TTATATCTTC CCTACTACGT TGCCATGGAG CTGTGCGGGT CAATCCTCCG 
CTCTTGTTAC CTAAATGTGG TTCAGAGAAT GATGCAAATG AAGCTGCAGT GCTAATAGGG 
GTTGGCGGTA ATGTCGTATC ACTGCCTTAT ATTCTAAGGA TTCCACTGGC TCGTTTTATT 
CTGTGTAAAG GAATCACAAA CATTAAAACT TACGCCATTG AAAAAGTTTT CCGAGCAAGA 
AAATATGCGA GGTCCCGACC TAAGGAATTA TGGGAATGTT CGTTTGACAT CATTTCCCCC 
TCAGTGGGAG GTCTCGCTCC CGACGCGGAA GTGATCTCTG TTGTTTCCGA GATCATCTCC 
CAATTCCCAT CTCTCTCCTC CCAGAAGTAC GAGGTTCGGA TCAACCACTT ATTGGCGCTA 
GAGTCAATAC TAACATACTG TGGTCTCGAT GAAAGCCACC ACACGGAGGC GCTCAATGCA 
CTCGCTTCAT GCAGTGGCCT CTCTAAAACA CAGCTCCACA ACTGCATCAA CAGCTTGCCT 
CTCAATGCTC AACAGGTTGC TTCCCTTCTT ACATTCATCC AAGTCCAAGG CCCGCTGTCC 
CGGGTTCAAA CCTCGCTGCG GTCGATTACC CGGAACAAGC ACTCACCCGC GTCGTCCAGA 
GCACGACAGG CGCTCCACGA ACTCGAAGCT CTTCGGAAAA ATCTTGATTC TTTAAACTTG 
ACACTACCTG TTGTGTTTGA TCTTGGTTAT TCTCATAAAC ACCACAACCA TTATAATGGC 
ATCATATTTA AAGTTTCAGC GGAAATCCAA AAGAAAAAAA GGACAGCAAT TGATGTGTTG 
GCCATCGGTG GTCGATATGA TAAACTGCTC ACAACCATGT CTGTATTCAA CATGTATGAC 
AGAGATGGGG AACAGAAGTT AACACCTAAG CATGCTGTGG GTGTTAGTAT TGCTGTGGAG 
AAAATTGTAG GATCCGTAAT GGAAGAAAAC AATCTTCCCA GCTCAACAAG TATGAGTGGC 
AGCAACGACC CCACTGTGAC ATCACAATTA TCCCACCACG CTGCAGTTGA CGTGTTGGTT 
TGTTCTGTCG GGCGAACTCT TCTGTTGCAC GAACGATTGA GGGTTGTAAG ATCTCTATGG 
GAGGCCTCGA TAGCAGCAGA AACCGTTTAT GATCGCGGAG CAATGGAATC AATGCAAGAA 
GTGCAGGAGA AAGCTCAAGA AAGAGGGATC CGCCATCTTG TATTTTTAAG CGAGAAAGAA 
AGTGGCTCCC CGTTAGTACG ATCATTGACG CACGACCCGC TGTTACGCTC TACTGAACGC 
AGAGTACCGC TGGATCAGTT GGTGGATTAT TTGTTAAAAC GATTGAAAGA AGTTGAATCA 
ACTGTATCAA TGAAATCTTC CTCATCGGGG TTCTCTGCAT CACACGAGTC CTCCACTGTT 
ACCATGACGA ACAATAGTGC TCGTCAAATC AGCATCGAGG TTGTTCCTCC CGAGAAACTT 
GCCTCACATT TAAGGAAGAG ATATGAGTCT GAAGTTATGA CCAAACTTAC AAGTCTACCT 
ATGATGTCCC ACATTCAATC TAAAGCTCAA ATTTGTGCCC TTGCGGTTGA ACTGCCATCA 
CCAGTTATGA AGAGCATGGG ATGTGCTCTT GATCTTTCTC ACTCTGAGGA AGAGTTTAAC 
TCTTCTCTTG CCCATCATCT ACACACTGTA TCCAAGTATA AAAAATACTT GTCCTATATC 
GCTGAGAGAA TTTGCGAGGC AAAATTCGAA CGAGAAATGA CTTTCATCAT TCTTTACAGC 
TACAAAGACC ATGGCTACTG TCTTTTGATA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018669175.1
CDS	107..5059
Translation	MTMTESESYQERQENELELLHSIFGEEIEDLRKLDKWKVVRPPEIILYLHPQEGMSGKKETFTKIDLKIKFTPTYPDSFPECKLENAKGLSEDAINDLESGIKNLYKSLQGEVMVLEIAQFVKEFLHTHNVPQCNSVYEEMVKNKQRQLEKAAQEEEKKKEIERRKKELKRKEIEEELARRQNEEKQIRKLMREGRDQNQSSQDHTRRLSEDIPNGNGSHGKSTGVVDLKINGKLPRIIHKGQTLDYIGRSTQKLKKIQKHIEICHLNTTSSGSNGSTVYAGMDANTGELVAVHYWNIPCPMRNSMLTDAERAKLDKYMKTINTIEQELNSLVKFSHKHLVPYLAMRYSHVESSIELHIVEKYITGASLQQKLTSRDRVGIDAVRTYSEQLLSALDCLHKKSVVHKNLKASSVFLSTKGSIKLADYSIEKRVIDLHNESRIEQPGVRFSEDRIPARNTKKGDILRLGMLILSLAQGQTIQDYPPEIPASFPLVFQNFLEKCLVVEDRLRYTTSELLDHEFIKPSVTSPILDIDLPLEQNPSPFDEDKMDDVLATSDAPRPTLSRLHSEFEKLEWLGKGGYGDVVKVKNIIDGRDYAIKRILLNPHRSLFNKKITREVKLLSRLNHENVVRYYNSWIETVECWEDDVSTNSSNPTTTTTTTNETKSNVSDRINSLGFYDNIEVLAPPPVASSVSDWSVSLEEVSRCRDDEEDDDEDVFKTIYVDEDDSESIKFDHSDEEEDEMVEESTNDGKTSRSEETTVTSSASSKSLVTQRFLYIQMEYCEKSTLRQTIDAGVCNEPERMWRLFREILEGLVHVHEQGMIHRDLKPQNIFLDKNDHVKIGDFGLATAQARVTLADSSEEISKSETGDSVYSGVVGTALYVAPELMVTGKRSLYSQKVDIYSVGIIFFEMSFPGIQTKMERVKVLGNLRTSSITFPSDFDEAAMPREAEITRWCLDHVPNKRPTASELLKCRLVPTPVLEESNVLEAVREMLSNPDSKPYRLVMDEVFNQPENPTSSVIYDIEMHKKHFSETQNVAQQQTFDIISSLLRCHGAVRVNPPLLLPKCGSENDANEAAVLIGVGGNVVSLPYILRIPLARFILCKGITNIKTYAIEKVFRARKYARSRPKELWECSFDIISPSVGGLAPDAEVISVVSEIISQFPSLSSQKYEVRINHLLALESILTYCGLDESHHTEALNALASCSGLSKTQLHNCINSLPLNAQQVASLLTFIQVQGPLSRVQTSLRSITRNKHSPASSRARQALHELEALRKNLDSLNLTLPVVFDLGYSHKHHNHYNGIIFKVSAEIQKKKRTAIDVLAIGGRYDKLLTTMSVFNMYDRDGEQKLTPKHAVGVSIAVEKIVGSVMEENNLPSSTSMSGSNDPTVTSQLSHHAAVDVLVCSVGRTLLLHERLRVVRSLWEASIAAETVYDRGAMESMQEVQEKAQERGIRHLVFLSEKESGSPLVRSLTHDPLLRSTERRVPLDQLVDYLLKRLKEVESTVSMKSSSSGFSASHESSTVTMTNNSARQISIEVVPPEKLASHLRKRYESEVMTKLTSLPMMSHIQSKAQICALAVELPSPVMKSMGCALDLSHSEEEFNSSLAHHLHTVSKYKKYLSYIAERICEAKFEREMTFIILYSYKDHGYCLLI

Target ORF information:

RefSeq Version	XM_018813630.1
Organism	Ciona intestinalis(vase tunicate)
Definition	Ciona intestinalis eIF-2-alpha kinase GCN2 (LOC100187226), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018813630.1

ORF Insert Sequence:

ATGACGATGA CGGAAAGTGA ATCCTATCAA GAAAGGCAGG AAAATGAACT TGAATTGCTT 
CACTCCATAT TTGGTGAGGA GATTGAAGAT CTGAGGAAAT TGGACAAATG GAAGGTGGTC 
CGTCCTCCAG AGATCATTCT CTATCTTCAT CCACAGGAAG GGATGAGCGG AAAGAAAGAA 
ACTTTTACCA AAATTGACCT CAAAATAAAA TTTACACCAA CTTACCCAGA CAGTTTCCCC 
GAGTGTAAAC TGGAGAATGC GAAAGGTTTA TCAGAAGATG CCATAAATGA TTTGGAATCT 
GGGATTAAAA ATCTCTACAA GTCATTACAG GGTGAAGTAA TGGTGCTCGA AATTGCTCAG 
TTTGTTAAGG AGTTCCTGCA TACACACAAT GTCCCACAAT GTAACTCGGT GTACGAGGAA 
ATGGTAAAAA ACAAACAAAG ACAGCTTGAA AAAGCTGCAC AGGAAGAGGA AAAGAAAAAG 
GAAATTGAAA GAAGAAAAAA GGAATTAAAG AGGAAAGAGA TAGAAGAAGA ACTAGCAAGG 
AGGCAAAACG AAGAAAAACA AATTCGTAAA CTTATGAGGG AGGGCAGAGA TCAGAACCAA 
TCATCTCAAG ACCACACACG CCGACTTTCT GAAGATATTC CGAACGGCAA TGGCAGTCAT 
GGGAAGTCTA CAGGAGTCGT GGATTTAAAA ATCAATGGAA AACTTCCACG AATAATACAT 
AAAGGGCAAA CATTAGACTA CATTGGAAGA TCCACACAAA AACTTAAAAA GATCCAGAAG 
CATATCGAGA TCTGCCATTT AAACACCACC AGTAGTGGTA GCAATGGATC AACAGTATAC 
GCGGGCATGG ATGCAAACAC AGGTGAACTA GTTGCAGTTC ATTACTGGAA CATACCATGT 
CCTATGAGGA ATTCAATGCT CACTGACGCA GAACGAGCTA AACTCGATAA ATATATGAAA 
ACAATTAACA CAATTGAGCA GGAGTTGAAT TCTTTGGTGA AATTCTCCCA CAAACACCTA 
GTGCCCTACC TTGCAATGAG ATATAGCCAT GTTGAAAGTA GTATTGAACT TCATATTGTG 
GAGAAATATA TAACAGGTGC AAGCCTTCAA CAAAAACTCA CAAGTAGAGA CAGAGTTGGA 
ATTGACGCTG TTAGAACTTA TTCTGAGCAA CTTTTATCTG CGCTTGACTG CCTACATAAA 
AAGTCTGTTG TACATAAGAA TCTTAAGGCG AGCAGTGTTT TCTTAAGCAC CAAGGGATCC 
ATCAAGCTTG CAGATTACAG CATAGAGAAA AGAGTAATAG ACTTACATAA TGAGTCCCGT 
ATTGAACAGC CTGGTGTTCG ATTCAGTGAA GATCGAATTC CTGCTCGAAA TACAAAGAAA 
GGAGATATTT TGCGGTTGGG TATGTTGATA CTGTCACTTG CACAAGGACA AACAATACAG 
GATTATCCTC CAGAAATACC AGCGAGCTTT CCACTGGTTT TCCAAAACTT TCTTGAGAAA 
TGTCTTGTGG TTGAAGACAG GCTTCGTTAT ACAACATCTG AACTCTTGGA TCATGAATTT 
ATCAAGCCAA GTGTCACCAG TCCTATCCTT GACATCGATC TTCCTTTGGA GCAAAATCCG 
AGTCCGTTTG ATGAAGATAA GATGGATGAT GTCCTTGCAA CATCCGATGC TCCAAGACCA 
ACTCTCTCTC GTCTCCACTC GGAGTTTGAG AAGTTAGAAT GGCTTGGGAA GGGAGGGTAT 
GGGGACGTGG TGAAGGTAAA GAACATAATA GACGGACGAG ATTACGCAAT CAAACGTATT 
CTACTGAACC CACATCGATC CCTGTTTAAT AAAAAGATAA CGAGAGAAGT GAAACTTTTG 
TCTCGGTTAA ACCATGAGAA TGTTGTCAGA TATTACAACT CGTGGATTGA AACAGTTGAA 
TGTTGGGAAG ATGATGTTTC AACAAATTCA TCTAACCCAA CAACAACAAC AACAACAACA 
AATGAAACAA AATCAAACGT ATCAGATCGT ATCAATTCAC TTGGTTTTTA TGATAATATT 
GAGGTTCTAG CGCCACCTCC TGTCGCATCT AGCGTGTCAG ATTGGAGCGT TTCATTGGAG 
GAGGTGAGTC GTTGTCGTGA CGACGAGGAA GATGATGACG AAGATGTGTT CAAGACTATC 
TATGTTGACG AGGATGACTC CGAGAGCATC AAGTTTGATC ACAGTGATGA AGAAGAAGAT 
GAGATGGTGG AAGAATCAAC TAATGATGGC AAAACGTCTC GGAGTGAAGA AACCACCGTC 
ACTAGTTCCG CGTCATCAAA ATCGCTTGTG ACTCAGCGAT TTCTATACAT ACAGATGGAA 
TATTGTGAGA AGAGCACACT GCGTCAAACC ATTGATGCTG GCGTTTGTAA TGAACCTGAG 
AGAATGTGGA GATTATTCAG GGAAATTCTT GAAGGATTGG TTCACGTACA TGAGCAAGGC 
ATGATCCATA GAGATCTAAA ACCACAGAAT ATTTTCCTCG ATAAAAACGA CCATGTTAAG 
ATTGGTGATT TTGGTCTTGC TACTGCACAA GCTAGAGTGA CACTAGCTGA CAGCTCTGAA 
GAGATTTCAA AATCCGAAAC TGGAGACAGT GTATATTCTG GTGTGGTGGG AACTGCATTA 
TACGTGGCAC CTGAACTTAT GGTTACTGGT AAAAGATCAC TGTACAGTCA AAAGGTTGAC 
ATCTACAGTG TTGGAATCAT TTTCTTTGAA ATGTCATTTC CTGGTATCCA AACTAAGATG 
GAACGAGTCA AAGTTCTTGG GAACCTTAGA ACTTCTTCCA TAACCTTCCC ATCTGACTTT 
GATGAAGCTG CCATGCCTCG TGAAGCTGAG ATAACTCGAT GGTGTTTGGA TCACGTGCCA 
AACAAACGAC CAACTGCATC TGAACTTCTA AAGTGTAGAT TAGTTCCAAC TCCTGTACTA 
GAAGAATCCA ATGTACTTGA AGCAGTTCGT GAAATGTTAA GCAATCCAGA CTCAAAGCCG 
TATAGATTAG TTATGGATGA AGTTTTCAAC CAACCAGAAA ACCCAACTTC ATCTGTTATA 
TACGACATTG AAATGCATAA GAAACATTTT TCTGAGACAC AGAATGTCGC ACAACAGCAA 
ACATTTGACA TTATATCTTC CCTACTACGT TGCCATGGAG CTGTGCGGGT CAATCCTCCG 
CTCTTGTTAC CTAAATGTGG TTCAGAGAAT GATGCAAATG AAGCTGCAGT GCTAATAGGG 
GTTGGCGGTA ATGTCGTATC ACTGCCTTAT ATTCTAAGGA TTCCACTGGC TCGTTTTATT 
CTGTGTAAAG GAATCACAAA CATTAAAACT TACGCCATTG AAAAAGTTTT CCGAGCAAGA 
AAATATGCGA GGTCCCGACC TAAGGAATTA TGGGAATGTT CGTTTGACAT CATTTCCCCC 
TCAGTGGGAG GTCTCGCTCC CGACGCGGAA GTGATCTCTG TTGTTTCCGA GATCATCTCC 
CAATTCCCAT CTCTCTCCTC CCAGAAGTAC GAGGTTCGGA TCAACCACTT ATTGGCGCTA 
GAGTCAATAC TAACATACTG TGGTCTCGAT GAAAGCCACC ACACGGAGGC GCTCAATGCA 
CTCGCTTCAT GCAGTGGCCT CTCTAAAACA CAGCTCCACA ACTGCATCAA CAGCTTGCCT 
CTCAATGCTC AACAGGTTGC TTCCCTTCTT ACATTCATCC AAGTCCAAGG CCCGCTGTCC 
CGGGTTCAAA CCTCGCTGCG GTCGATTACC CGGAACAAGC ACTCACCCGC GTCGTCCAGA 
GCACGACAGG CGCTCCACGA ACTCGAAGCT CTTCGGAAAA ATCTTGATTC TTTAAACTTG 
ACACTACCTG TTGTGTTTGA TCTTGGTTAT TCTCATAAAC ACCACAACCA TTATAATGGC 
ATCATATTTA AAGTTTCAGC GGAAATCCAA AAGAAAAAAA GGACAGCAAT TGATGTGTTG 
GCCATCGGTG GTCGATATGA TAAACTGCTC ACAACCATGT CTGTATTCAA CATGTATGAC 
AGAGATGGGG AACAGAAGTT AACACCTAAG CATGCTGTGG GTGTTAGTAT TGCTGTGGAG 
AAAATTGTAG GATCCGTAAT GGAAGAAAAC AATCTTCCCA GCTCAACAAG TATGAGTGGC 
AGCAACGACC CCACTGTGAC ATCACAATTA TCCCACCACG CTGCAGTTGA CGTGTTGGTT 
TGTTCTGTCG GGCGAACTCT TCTGTTGCAC GAACGATTGA GGGTTGTAAG ATCTCTATGG 
GAGGCCTCGA TAGCAGCAGA AACCGTTTAT GATCGCGGAG CAATGGAATC AATGCAAGAA 
GTGCAGGAGA AAGCTCAAGA AAGAGGGATC CGCCATCTTG TATTTTTAAG CGAGAAAGAA 
AGTGGCTCCC CGTTAGTACG ATCATTGACG CACGACCCGC TGTTACGCTC TACTGAACGC 
AGAGTACCGC TGGATCAGTT GGTGGATTAT TTGTTAAAAC GATTGAAAGA AGTTGAATCA 
ACTGTATCAA TGAAATCTTC CTCATCGGGG TTCTCTGCAT CACACGAGTC CTCCACTGTT 
ACCATGACGA ACAATAGTGC TCGTCAAATC AGCATCGAGG TTGTTCCTCC CGAGAAACTT 
GCCTCACATT TAAGGAAGAG ATATGAGTCT GAAGTTATGA CCAAACTTAC AAGTCTACCT 
ATGATGTCCC ACATTCAATC TAAAGCTCAA ATTTGTGCCC TTGCGGTTGA ACTGCCATCA 
CCAGTTATGA AGAGCATGGG ATGTGCTCTT GATCTTTCTC ACTCTGAGGA AGAGTTTAAC 
TCTTCTCTTG CCCATCATCT ACACACTGTA TCCAAGTATA AAAAATACTT GTCCTATATC 
GCTGAGAGAA TTTGCGAGGC AAAATTCGAA CGAGAAATGA CTTTCATCAT TCTTTACAGC 
TACAAAGACC ATGGCTACTG TCTTTTGATA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100187226 ( XM_018813631.1 ) cDNA ORF clone, Ciona intestinalis(vase tunicate) -> XP_018669176.1 Ciona intestinalis eIF-2-alpha kinase GCN2 (LOC100187226), transcript variant X3, mRNA.

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CloneID	OCi70882
Clone ID Related Accession (Same CDS sequence)	XM_018813631.1 , XM_018813631.2
Accession Version	XM_018813631.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-20
Organism	Ciona intestinalis(vase tunicate)
Product	eIF-2-alpha kinase GCN2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_020175.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ciona intestinalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGATGA CGGAAAGTGA ATCCTATCAA GAAAGGCAGG AAAATGAACT TGAATTGCTT 
CACTCCATAT TTGGTGAGGA GATTGAAGAT CTGAGGAAAT TGGACAAATG GAAGGTGGTC 
CGTCCTCCAG AGATCATTCT CTATCTTCAT CCACAGGAAG GGATGAGCGG AAAGAAAGAA 
ACTTTTACCA AAATTGACCT CAAAATAAAA TTTACACCAA CTTACCCAGA CAGTTTCCCC 
GAGTGTAAAC TGGAGAATGC GAAAGGTTTA TCAGAAGATG CCATAAATGA TTTGGAATCT 
GGGATTAAAA ATCTCTACAA GTCATTACAG GGTGAAGTAA TGGTGCTCGA AATTGCTCAG 
TTTGTTAAGG AGTTCCTGCA TACACACAAT GTCCCACAAT GTAACTCGGT GTACGAGGAA 
ATGGTAAAAA ACAAACAAAG ACAGCTTGAA AAAGCTGCAC AGGAAGAGGA AAAGAAAAAG 
GAAATTGAAA GAAGAAAAAA GGAATTAAAG AGGAAAGAGA TAGAAGAAGA ACTAGCAAGG 
AGGCAAAACG AAGAAAAACA AATTCGTAAA CTTATGAGGG AGGGCAGAGA TCAGAACCAA 
TCATCTCAAG ACCACACACG CCGACTTTCT GAAGATATTC CGAACGGCAA TGGCAGTCAT 
GGGAAGTCTA CAGGAGTCGT GGATTTAAAA ATCAATGGAA AACTTCCACG AATAATACAT 
AAAGGGCAAA CATTAGACTA CATTGGAAGA TCCACACAAA AACTTAAAAA GATCCAGAAG 
CATATCGAGA TCTGCCATTT AAACACCACC AGTAGTGGTA GCAATGGATC AACAGTATAC 
GCGGGCATGG ATGCAAACAC AGGTGAACTA GTTGCAGTTC ATTACTGGAA CATACCATGT 
CCTATGAGGA ATTCAATGCT CACTGACGCA GAACGAGCTA AACTCGATAA ATATATGAAA 
ACAATTAACA CAATTGAGCA GGAGTTGAAT TCTTTGGTGA AATTCTCCCA CAAACACCTA 
GTGCCCTACC TTGCAATGAG ATATAGCCAT GTTGAAAGTA GTATTGAACT TCATATTGTG 
GAGAAATATA TAACAGGTGC AAGCCTTCAA CAAAAACTCA CAAGTAGAGA CAGAGTTGGA 
ATTGACGCTG TTAGAACTTA TTCTGAGCAA CTTTTATCTG CGCTTGACTG CCTACATAAA 
AAGTCTGTTG TACATAAGAA TCTTAAGGCG AGCAGTGTTT TCTTAAGCAC CAAGGGATCC 
ATCAAGCTTG CAGATTACAG CATAGAGAAA AGAGTAATAG ACTTACATAA TGAGTCCCGT 
ATTGAACAGC CTGGTGTTCG ATTCAGTGAA GATCGAATTC CTGCTCGAAA TACAAAGAAA 
GGAGATATTT TGCGGTTGGG TATGTTGATA CTGTCACTTG CACAAGGACA AACAATACAG 
GATTATCCTC CAGAAATACC AGCGAGCTTT CCACTGGTTT TCCAAAACTT TCTTGAGAAA 
TGTCTTGTGG TTGAAGACAG GCTTCGTTAT ACAACATCTG AACTCTTGGA TCATGAATTT 
ATCAAGCCAA GTGTCACCAG TCCTATCCTT GACATCGATC TTCCTTTGGA GCAAAATCCG 
AGTCCGTTTG ATGAAGATAA GATGGATGAT GTCCTTGCAA CATCCGATGC TCCAAGACCA 
ACTCTCTCTC GTCTCCACTC GGAGTTTGAG AAGTTAGAAT GGCTTGGGAA GGGAGGGTAT 
GGGGACGTGG TGAAGGTAAA GAACATAATA GACGGACGAG ATTACGCAAT CAAACGTATT 
CTACTGAACC CACATCGATC CCTGTTTAAT AAAAAGATAA CGAGAGAAGT GAAACTTTTG 
TCTCGGTTAA ACCATGAGAA TGTTGTCAGA TATTACAACT CGTGGATTGA AACAGTTGAA 
TGTTGGGAAG ATGATGTTTC AACAAATTCA TCTAACCCAA CAACAACAAC AACAACAACA 
AATGAAACAA AATCAAACGT ATCAGATCGT ATCAATTCAC TTGGTTTTTA TGATAATATT 
GAGGTTCTAG CGCCACCTCC TGTCGCATCT AGCGTGTCAG ATTGGAGCGT TTCATTGGAG 
GAGGTGAGTC GTTGTCGTGA CGACGAGGAA GATGATGACG AAGATGTGTT CAAGACTATC 
TATGTTGACG AGGATGACTC CGAGAGCATC AAGTTTGATC ACAGTGATGA AGAAGAAGAT 
GAGATGGTGG AAGAATCAAC TAATGATGGC AAAACGTCTC GGAGTGAAGA AACCACCGTC 
ACTAGTTCCG CGTCATCAAA ATCGCTTGTG ACTCAGCGAT TTCTATACAT ACAGATGGAA 
TATTGTGAGA AGAGCACACT GCGTCAAACC ATTGATGCTG GCGTTTGTAA TGAACCTGAG 
AGAATGTGGA GATTATTCAG GGAAATTCTT GAAGGATTGG TTCACGTACA TGAGCAAGGC 
ATGATCCATA GAGATCTAAA ACCACAGAAT ATTTTCCTCG ATAAAAACGA CCATGTTAAG 
ATTGGTGATT TTGGTCTTGC TACTGCACAA GCTAGAGTGA CACTAGCTGA CAGCTCTGAA 
GAGATTTCAA AATCCGAAAC TGGAGACAGT GTATATTCTG GTGTGGTGGG AACTGCATTA 
TACGTGGCAC CTGAACTTAT GGTTACTGGT AAAAGATCAC TGTACAGTCA AAAGGTTGAC 
ATCTACAGTG TTGGAATCAT TTTCTTTGAA ATGTCATTTC CTGGTATCCA AACTAAGATG 
GAACGAGTCA AAGTTCTTGG GAACCTTAGA ACTTCTTCCA TAACCTTCCC ATCTGACTTT 
GATGAAGCTG CCATGCCTCG TGAAGCTGAG ATAACTCGAT GGTGTTTGGA TCACGTGCCA 
AACAAACGAC CAACTGCATC TGAACTTCTA AAGTGTAGAT TAGTTCCAAC TCCTGTACTA 
GAAGAATCCA ATGTACTTGA AGCAGTTCGT GAAATGTTAA GCAATCCAGA CTCAAAGCCG 
TATAGATTAG TTATGGATGA AGTTTTCAAC CAACCAGAAA ACCCAACTTC ATCTGTTATA 
TACGACATTG AAATGCATAA GAAACATTTT TCTGAGACAC AGAATGTCGC ACAACAGCAA 
ACATTTGACA TTATATCTTC CCTACTACGT TGCCATGGAG CTGTGCGGGT CAATCCTCCG 
CTCTTGTTAC CTAAATGTGG TTCAGAGAAT GATGCAAATG AAGCTGCAGT GCTAATAGGG 
GTTGGCGGTA ATGTCGTATC ACTGCCTTAT ATTCTAAGGA TTCCACTGGC TCGTTTTATT 
CTGTGTAAAG GAATCACAAA CATTAAAACT TACGCCATTG AAAAAGTTTT CCGAGCAAGA 
AAATATGCGA GGTCCCGACC TAAGGAATTA TGGGAATGTT CGTTTGACAT CATTTCCCCC 
TCAGTGGGAG GTCTCGCTCC CGACGCGGAA GTGATCTCTG TTGTTTCCGA GATCATCTCC 
CAATTCCCAT CTCTCTCCTC CCAGAAGTAC GAGGTTCGGA TCAACCACTT ATTGGCGCTA 
GAGTCAATAC TAACATACTG TGGTCTCGAT GAAAGCCACC ACACGGAGGC GCTCAATGCA 
CTCGCTTCAT GCAGTGGCCT CTCTAAAACA CAGCTCCACA ACTGCATCAA CAGCTTGCCT 
CTCAATGCTC AACAGGTTGC TTCCCTTCTT ACATTCATCC AAGTCCAAGG CCCGCTGTCC 
CGGGTTCAAA CCTCGCTGCG GTCGATTACC CGGAACAAGC ACTCACCCGC GTCGTCCAGA 
GCACGACAGG CGCTCCACGA ACTCGAAGCT CTTCGGAAAA ATCTTGATTC TTTAAACTTG 
ACACTACCTG TTGTGTTTGA TCTTGGTTAT TCTCATAAAC ACCACAACCA TTATAATGGC 
ATCATATTTA AAGTTTCAGC GGAAATCCAA AAGAAAAAAA GGACAGCAAT TGATGTGTTG 
GCCATCGGTG GTCGATATGA TAAACTGCTC ACAACCATGT CTGTATTCAA CATGTATGAC 
AGAGATGGGG AACAGAAGTT AACACCTAAG CATGCTGTGG GTGTTAGTAT TGCTGTGGAG 
AAAATTGTAG GATCCGTAAT GGAAGAAAAC AGTCTTTGTA GCTCAACAAG TATGAGTGGC 
AGCAATGACC CCACTGTGAC ATCACAATTA TCCCACCACG CTGCAGTTGA CGTGCTGGTT 
TGTTCCGTGG GGCGGACTCT TATGTTGCAC GAACGATTGA GGGTTGTAAG ATCTCTATGG 
GAGGCCTCGA TAGCAGCAGA AACCGTTTAT GATCGCGGGG CAATGGAATC AATGCAAGAA 
GTGCAGGAGA AAGCTCAAGA AAGAGGGATC CGCCATCTTG TATTTTTAAG CGAGAAAGAA 
AGTGGCTCCC CGTTAGTACG ATCATTGACG CACGACCCGC TGTTACGCTC TACTGAACGC 
AGAGTACCGC TGGATCAGTT GGTGGATTAT TTGTTAAAAC GATTGAAAGA AGTTGAATCA 
ACTGTATCAA TGAAATCTTC CTCATCGGGG TTCTCTGCAT CACACGAGTC CTCCACTGTT 
ACCATGACGA ACAATAGTGC TCGTCAAATC AGCATCGAGG TTGTTCCTCC CGAGAAACTT 
GCCTCACATT TAAGGAAGAG ATATGAGTCT GAAGTTATGA CCAAACTTAC AAGTCTACCT 
ATGATGTCCC ACATTCAATC TAAAGCTCAA ATTTGTGCCC TTGCGGTTGA ACTGCCATCA 
CCAGTTATGA AGAGCATGGG ATGTGCTCTT GATCTTTCTC ACTCTGAGGA AGAGTTTAAC 
TCTTCTCTTG CCCATCATCT ACACACTGTA TCCAAGTATA AAAAATACTT GTCCTATATC 
GCTGAGAGAA TTTGCGAGGC AAAATTCGAA CGAGAAATGA CTTTCATCAT TCTTTACAGC 
TACAAAGACC ATGGCTACTG TCTTTTGATA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018669176.1
CDS	107..5059
Translation	MTMTESESYQERQENELELLHSIFGEEIEDLRKLDKWKVVRPPEIILYLHPQEGMSGKKETFTKIDLKIKFTPTYPDSFPECKLENAKGLSEDAINDLESGIKNLYKSLQGEVMVLEIAQFVKEFLHTHNVPQCNSVYEEMVKNKQRQLEKAAQEEEKKKEIERRKKELKRKEIEEELARRQNEEKQIRKLMREGRDQNQSSQDHTRRLSEDIPNGNGSHGKSTGVVDLKINGKLPRIIHKGQTLDYIGRSTQKLKKIQKHIEICHLNTTSSGSNGSTVYAGMDANTGELVAVHYWNIPCPMRNSMLTDAERAKLDKYMKTINTIEQELNSLVKFSHKHLVPYLAMRYSHVESSIELHIVEKYITGASLQQKLTSRDRVGIDAVRTYSEQLLSALDCLHKKSVVHKNLKASSVFLSTKGSIKLADYSIEKRVIDLHNESRIEQPGVRFSEDRIPARNTKKGDILRLGMLILSLAQGQTIQDYPPEIPASFPLVFQNFLEKCLVVEDRLRYTTSELLDHEFIKPSVTSPILDIDLPLEQNPSPFDEDKMDDVLATSDAPRPTLSRLHSEFEKLEWLGKGGYGDVVKVKNIIDGRDYAIKRILLNPHRSLFNKKITREVKLLSRLNHENVVRYYNSWIETVECWEDDVSTNSSNPTTTTTTTNETKSNVSDRINSLGFYDNIEVLAPPPVASSVSDWSVSLEEVSRCRDDEEDDDEDVFKTIYVDEDDSESIKFDHSDEEEDEMVEESTNDGKTSRSEETTVTSSASSKSLVTQRFLYIQMEYCEKSTLRQTIDAGVCNEPERMWRLFREILEGLVHVHEQGMIHRDLKPQNIFLDKNDHVKIGDFGLATAQARVTLADSSEEISKSETGDSVYSGVVGTALYVAPELMVTGKRSLYSQKVDIYSVGIIFFEMSFPGIQTKMERVKVLGNLRTSSITFPSDFDEAAMPREAEITRWCLDHVPNKRPTASELLKCRLVPTPVLEESNVLEAVREMLSNPDSKPYRLVMDEVFNQPENPTSSVIYDIEMHKKHFSETQNVAQQQTFDIISSLLRCHGAVRVNPPLLLPKCGSENDANEAAVLIGVGGNVVSLPYILRIPLARFILCKGITNIKTYAIEKVFRARKYARSRPKELWECSFDIISPSVGGLAPDAEVISVVSEIISQFPSLSSQKYEVRINHLLALESILTYCGLDESHHTEALNALASCSGLSKTQLHNCINSLPLNAQQVASLLTFIQVQGPLSRVQTSLRSITRNKHSPASSRARQALHELEALRKNLDSLNLTLPVVFDLGYSHKHHNHYNGIIFKVSAEIQKKKRTAIDVLAIGGRYDKLLTTMSVFNMYDRDGEQKLTPKHAVGVSIAVEKIVGSVMEENSLCSSTSMSGSNDPTVTSQLSHHAAVDVLVCSVGRTLMLHERLRVVRSLWEASIAAETVYDRGAMESMQEVQEKAQERGIRHLVFLSEKESGSPLVRSLTHDPLLRSTERRVPLDQLVDYLLKRLKEVESTVSMKSSSSGFSASHESSTVTMTNNSARQISIEVVPPEKLASHLRKRYESEVMTKLTSLPMMSHIQSKAQICALAVELPSPVMKSMGCALDLSHSEEEFNSSLAHHLHTVSKYKKYLSYIAERICEAKFEREMTFIILYSYKDHGYCLLI

Target ORF information:

RefSeq Version	XM_018813631.1
Organism	Ciona intestinalis(vase tunicate)
Definition	Ciona intestinalis eIF-2-alpha kinase GCN2 (LOC100187226), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018813631.1

ORF Insert Sequence:

ATGACGATGA CGGAAAGTGA ATCCTATCAA GAAAGGCAGG AAAATGAACT TGAATTGCTT 
CACTCCATAT TTGGTGAGGA GATTGAAGAT CTGAGGAAAT TGGACAAATG GAAGGTGGTC 
CGTCCTCCAG AGATCATTCT CTATCTTCAT CCACAGGAAG GGATGAGCGG AAAGAAAGAA 
ACTTTTACCA AAATTGACCT CAAAATAAAA TTTACACCAA CTTACCCAGA CAGTTTCCCC 
GAGTGTAAAC TGGAGAATGC GAAAGGTTTA TCAGAAGATG CCATAAATGA TTTGGAATCT 
GGGATTAAAA ATCTCTACAA GTCATTACAG GGTGAAGTAA TGGTGCTCGA AATTGCTCAG 
TTTGTTAAGG AGTTCCTGCA TACACACAAT GTCCCACAAT GTAACTCGGT GTACGAGGAA 
ATGGTAAAAA ACAAACAAAG ACAGCTTGAA AAAGCTGCAC AGGAAGAGGA AAAGAAAAAG 
GAAATTGAAA GAAGAAAAAA GGAATTAAAG AGGAAAGAGA TAGAAGAAGA ACTAGCAAGG 
AGGCAAAACG AAGAAAAACA AATTCGTAAA CTTATGAGGG AGGGCAGAGA TCAGAACCAA 
TCATCTCAAG ACCACACACG CCGACTTTCT GAAGATATTC CGAACGGCAA TGGCAGTCAT 
GGGAAGTCTA CAGGAGTCGT GGATTTAAAA ATCAATGGAA AACTTCCACG AATAATACAT 
AAAGGGCAAA CATTAGACTA CATTGGAAGA TCCACACAAA AACTTAAAAA GATCCAGAAG 
CATATCGAGA TCTGCCATTT AAACACCACC AGTAGTGGTA GCAATGGATC AACAGTATAC 
GCGGGCATGG ATGCAAACAC AGGTGAACTA GTTGCAGTTC ATTACTGGAA CATACCATGT 
CCTATGAGGA ATTCAATGCT CACTGACGCA GAACGAGCTA AACTCGATAA ATATATGAAA 
ACAATTAACA CAATTGAGCA GGAGTTGAAT TCTTTGGTGA AATTCTCCCA CAAACACCTA 
GTGCCCTACC TTGCAATGAG ATATAGCCAT GTTGAAAGTA GTATTGAACT TCATATTGTG 
GAGAAATATA TAACAGGTGC AAGCCTTCAA CAAAAACTCA CAAGTAGAGA CAGAGTTGGA 
ATTGACGCTG TTAGAACTTA TTCTGAGCAA CTTTTATCTG CGCTTGACTG CCTACATAAA 
AAGTCTGTTG TACATAAGAA TCTTAAGGCG AGCAGTGTTT TCTTAAGCAC CAAGGGATCC 
ATCAAGCTTG CAGATTACAG CATAGAGAAA AGAGTAATAG ACTTACATAA TGAGTCCCGT 
ATTGAACAGC CTGGTGTTCG ATTCAGTGAA GATCGAATTC CTGCTCGAAA TACAAAGAAA 
GGAGATATTT TGCGGTTGGG TATGTTGATA CTGTCACTTG CACAAGGACA AACAATACAG 
GATTATCCTC CAGAAATACC AGCGAGCTTT CCACTGGTTT TCCAAAACTT TCTTGAGAAA 
TGTCTTGTGG TTGAAGACAG GCTTCGTTAT ACAACATCTG AACTCTTGGA TCATGAATTT 
ATCAAGCCAA GTGTCACCAG TCCTATCCTT GACATCGATC TTCCTTTGGA GCAAAATCCG 
AGTCCGTTTG ATGAAGATAA GATGGATGAT GTCCTTGCAA CATCCGATGC TCCAAGACCA 
ACTCTCTCTC GTCTCCACTC GGAGTTTGAG AAGTTAGAAT GGCTTGGGAA GGGAGGGTAT 
GGGGACGTGG TGAAGGTAAA GAACATAATA GACGGACGAG ATTACGCAAT CAAACGTATT 
CTACTGAACC CACATCGATC CCTGTTTAAT AAAAAGATAA CGAGAGAAGT GAAACTTTTG 
TCTCGGTTAA ACCATGAGAA TGTTGTCAGA TATTACAACT CGTGGATTGA AACAGTTGAA 
TGTTGGGAAG ATGATGTTTC AACAAATTCA TCTAACCCAA CAACAACAAC AACAACAACA 
AATGAAACAA AATCAAACGT ATCAGATCGT ATCAATTCAC TTGGTTTTTA TGATAATATT 
GAGGTTCTAG CGCCACCTCC TGTCGCATCT AGCGTGTCAG ATTGGAGCGT TTCATTGGAG 
GAGGTGAGTC GTTGTCGTGA CGACGAGGAA GATGATGACG AAGATGTGTT CAAGACTATC 
TATGTTGACG AGGATGACTC CGAGAGCATC AAGTTTGATC ACAGTGATGA AGAAGAAGAT 
GAGATGGTGG AAGAATCAAC TAATGATGGC AAAACGTCTC GGAGTGAAGA AACCACCGTC 
ACTAGTTCCG CGTCATCAAA ATCGCTTGTG ACTCAGCGAT TTCTATACAT ACAGATGGAA 
TATTGTGAGA AGAGCACACT GCGTCAAACC ATTGATGCTG GCGTTTGTAA TGAACCTGAG 
AGAATGTGGA GATTATTCAG GGAAATTCTT GAAGGATTGG TTCACGTACA TGAGCAAGGC 
ATGATCCATA GAGATCTAAA ACCACAGAAT ATTTTCCTCG ATAAAAACGA CCATGTTAAG 
ATTGGTGATT TTGGTCTTGC TACTGCACAA GCTAGAGTGA CACTAGCTGA CAGCTCTGAA 
GAGATTTCAA AATCCGAAAC TGGAGACAGT GTATATTCTG GTGTGGTGGG AACTGCATTA 
TACGTGGCAC CTGAACTTAT GGTTACTGGT AAAAGATCAC TGTACAGTCA AAAGGTTGAC 
ATCTACAGTG TTGGAATCAT TTTCTTTGAA ATGTCATTTC CTGGTATCCA AACTAAGATG 
GAACGAGTCA AAGTTCTTGG GAACCTTAGA ACTTCTTCCA TAACCTTCCC ATCTGACTTT 
GATGAAGCTG CCATGCCTCG TGAAGCTGAG ATAACTCGAT GGTGTTTGGA TCACGTGCCA 
AACAAACGAC CAACTGCATC TGAACTTCTA AAGTGTAGAT TAGTTCCAAC TCCTGTACTA 
GAAGAATCCA ATGTACTTGA AGCAGTTCGT GAAATGTTAA GCAATCCAGA CTCAAAGCCG 
TATAGATTAG TTATGGATGA AGTTTTCAAC CAACCAGAAA ACCCAACTTC ATCTGTTATA 
TACGACATTG AAATGCATAA GAAACATTTT TCTGAGACAC AGAATGTCGC ACAACAGCAA 
ACATTTGACA TTATATCTTC CCTACTACGT TGCCATGGAG CTGTGCGGGT CAATCCTCCG 
CTCTTGTTAC CTAAATGTGG TTCAGAGAAT GATGCAAATG AAGCTGCAGT GCTAATAGGG 
GTTGGCGGTA ATGTCGTATC ACTGCCTTAT ATTCTAAGGA TTCCACTGGC TCGTTTTATT 
CTGTGTAAAG GAATCACAAA CATTAAAACT TACGCCATTG AAAAAGTTTT CCGAGCAAGA 
AAATATGCGA GGTCCCGACC TAAGGAATTA TGGGAATGTT CGTTTGACAT CATTTCCCCC 
TCAGTGGGAG GTCTCGCTCC CGACGCGGAA GTGATCTCTG TTGTTTCCGA GATCATCTCC 
CAATTCCCAT CTCTCTCCTC CCAGAAGTAC GAGGTTCGGA TCAACCACTT ATTGGCGCTA 
GAGTCAATAC TAACATACTG TGGTCTCGAT GAAAGCCACC ACACGGAGGC GCTCAATGCA 
CTCGCTTCAT GCAGTGGCCT CTCTAAAACA CAGCTCCACA ACTGCATCAA CAGCTTGCCT 
CTCAATGCTC AACAGGTTGC TTCCCTTCTT ACATTCATCC AAGTCCAAGG CCCGCTGTCC 
CGGGTTCAAA CCTCGCTGCG GTCGATTACC CGGAACAAGC ACTCACCCGC GTCGTCCAGA 
GCACGACAGG CGCTCCACGA ACTCGAAGCT CTTCGGAAAA ATCTTGATTC TTTAAACTTG 
ACACTACCTG TTGTGTTTGA TCTTGGTTAT TCTCATAAAC ACCACAACCA TTATAATGGC 
ATCATATTTA AAGTTTCAGC GGAAATCCAA AAGAAAAAAA GGACAGCAAT TGATGTGTTG 
GCCATCGGTG GTCGATATGA TAAACTGCTC ACAACCATGT CTGTATTCAA CATGTATGAC 
AGAGATGGGG AACAGAAGTT AACACCTAAG CATGCTGTGG GTGTTAGTAT TGCTGTGGAG 
AAAATTGTAG GATCCGTAAT GGAAGAAAAC AGTCTTTGTA GCTCAACAAG TATGAGTGGC 
AGCAATGACC CCACTGTGAC ATCACAATTA TCCCACCACG CTGCAGTTGA CGTGCTGGTT 
TGTTCCGTGG GGCGGACTCT TATGTTGCAC GAACGATTGA GGGTTGTAAG ATCTCTATGG 
GAGGCCTCGA TAGCAGCAGA AACCGTTTAT GATCGCGGGG CAATGGAATC AATGCAAGAA 
GTGCAGGAGA AAGCTCAAGA AAGAGGGATC CGCCATCTTG TATTTTTAAG CGAGAAAGAA 
AGTGGCTCCC CGTTAGTACG ATCATTGACG CACGACCCGC TGTTACGCTC TACTGAACGC 
AGAGTACCGC TGGATCAGTT GGTGGATTAT TTGTTAAAAC GATTGAAAGA AGTTGAATCA 
ACTGTATCAA TGAAATCTTC CTCATCGGGG TTCTCTGCAT CACACGAGTC CTCCACTGTT 
ACCATGACGA ACAATAGTGC TCGTCAAATC AGCATCGAGG TTGTTCCTCC CGAGAAACTT 
GCCTCACATT TAAGGAAGAG ATATGAGTCT GAAGTTATGA CCAAACTTAC AAGTCTACCT 
ATGATGTCCC ACATTCAATC TAAAGCTCAA ATTTGTGCCC TTGCGGTTGA ACTGCCATCA 
CCAGTTATGA AGAGCATGGG ATGTGCTCTT GATCTTTCTC ACTCTGAGGA AGAGTTTAAC 
TCTTCTCTTG CCCATCATCT ACACACTGTA TCCAAGTATA AAAAATACTT GTCCTATATC 
GCTGAGAGAA TTTGCGAGGC AAAATTCGAA CGAGAAATGA CTTTCATCAT TCTTTACAGC 
TACAAAGACC ATGGCTACTG TCTTTTGATA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100187226 ( XM_018813631.2 ) cDNA ORF clone, Ciona intestinalis(vase tunicate) -> XP_018669176.1 Ciona intestinalis eIF-2-alpha kinase GCN2 (LOC100187226), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCi70882
Clone ID Related Accession (Same CDS sequence)	XM_018813631.1 , XM_018813631.2
Accession Version	XM_018813631.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-21
Organism	Ciona intestinalis(vase tunicate)
Product	eIF-2-alpha kinase GCN2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_020175.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Oct 22, 2018 this sequence version replaced XM_018813631.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ciona intestinalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGATGA CGGAAAGTGA ATCCTATCAA GAAAGGCAGG AAAATGAACT TGAATTGCTT 
CACTCCATAT TTGGTGAGGA GATTGAAGAT CTGAGGAAAT TGGACAAATG GAAGGTGGTC 
CGTCCTCCAG AGATCATTCT CTATCTTCAT CCACAGGAAG GGATGAGCGG AAAGAAAGAA 
ACTTTTACCA AAATTGACCT CAAAATAAAA TTTACACCAA CTTACCCAGA CAGTTTCCCC 
GAGTGTAAAC TGGAGAATGC GAAAGGTTTA TCAGAAGATG CCATAAATGA TTTGGAATCT 
GGGATTAAAA ATCTCTACAA GTCATTACAG GGTGAAGTAA TGGTGCTCGA AATTGCTCAG 
TTTGTTAAGG AGTTCCTGCA TACACACAAT GTCCCACAAT GTAACTCGGT GTACGAGGAA 
ATGGTAAAAA ACAAACAAAG ACAGCTTGAA AAAGCTGCAC AGGAAGAGGA AAAGAAAAAG 
GAAATTGAAA GAAGAAAAAA GGAATTAAAG AGGAAAGAGA TAGAAGAAGA ACTAGCAAGG 
AGGCAAAACG AAGAAAAACA AATTCGTAAA CTTATGAGGG AGGGCAGAGA TCAGAACCAA 
TCATCTCAAG ACCACACACG CCGACTTTCT GAAGATATTC CGAACGGCAA TGGCAGTCAT 
GGGAAGTCTA CAGGAGTCGT GGATTTAAAA ATCAATGGAA AACTTCCACG AATAATACAT 
AAAGGGCAAA CATTAGACTA CATTGGAAGA TCCACACAAA AACTTAAAAA GATCCAGAAG 
CATATCGAGA TCTGCCATTT AAACACCACC AGTAGTGGTA GCAATGGATC AACAGTATAC 
GCGGGCATGG ATGCAAACAC AGGTGAACTA GTTGCAGTTC ATTACTGGAA CATACCATGT 
CCTATGAGGA ATTCAATGCT CACTGACGCA GAACGAGCTA AACTCGATAA ATATATGAAA 
ACAATTAACA CAATTGAGCA GGAGTTGAAT TCTTTGGTGA AATTCTCCCA CAAACACCTA 
GTGCCCTACC TTGCAATGAG ATATAGCCAT GTTGAAAGTA GTATTGAACT TCATATTGTG 
GAGAAATATA TAACAGGTGC AAGCCTTCAA CAAAAACTCA CAAGTAGAGA CAGAGTTGGA 
ATTGACGCTG TTAGAACTTA TTCTGAGCAA CTTTTATCTG CGCTTGACTG CCTACATAAA 
AAGTCTGTTG TACATAAGAA TCTTAAGGCG AGCAGTGTTT TCTTAAGCAC CAAGGGATCC 
ATCAAGCTTG CAGATTACAG CATAGAGAAA AGAGTAATAG ACTTACATAA TGAGTCCCGT 
ATTGAACAGC CTGGTGTTCG ATTCAGTGAA GATCGAATTC CTGCTCGAAA TACAAAGAAA 
GGAGATATTT TGCGGTTGGG TATGTTGATA CTGTCACTTG CACAAGGACA AACAATACAG 
GATTATCCTC CAGAAATACC AGCGAGCTTT CCACTGGTTT TCCAAAACTT TCTTGAGAAA 
TGTCTTGTGG TTGAAGACAG GCTTCGTTAT ACAACATCTG AACTCTTGGA TCATGAATTT 
ATCAAGCCAA GTGTCACCAG TCCTATCCTT GACATCGATC TTCCTTTGGA GCAAAATCCG 
AGTCCGTTTG ATGAAGATAA GATGGATGAT GTCCTTGCAA CATCCGATGC TCCAAGACCA 
ACTCTCTCTC GTCTCCACTC GGAGTTTGAG AAGTTAGAAT GGCTTGGGAA GGGAGGGTAT 
GGGGACGTGG TGAAGGTAAA GAACATAATA GACGGACGAG ATTACGCAAT CAAACGTATT 
CTACTGAACC CACATCGATC CCTGTTTAAT AAAAAGATAA CGAGAGAAGT GAAACTTTTG 
TCTCGGTTAA ACCATGAGAA TGTTGTCAGA TATTACAACT CGTGGATTGA AACAGTTGAA 
TGTTGGGAAG ATGATGTTTC AACAAATTCA TCTAACCCAA CAACAACAAC AACAACAACA 
AATGAAACAA AATCAAACGT ATCAGATCGT ATCAATTCAC TTGGTTTTTA TGATAATATT 
GAGGTTCTAG CGCCACCTCC TGTCGCATCT AGCGTGTCAG ATTGGAGCGT TTCATTGGAG 
GAGGTGAGTC GTTGTCGTGA CGACGAGGAA GATGATGACG AAGATGTGTT CAAGACTATC 
TATGTTGACG AGGATGACTC CGAGAGCATC AAGTTTGATC ACAGTGATGA AGAAGAAGAT 
GAGATGGTGG AAGAATCAAC TAATGATGGC AAAACGTCTC GGAGTGAAGA AACCACCGTC 
ACTAGTTCCG CGTCATCAAA ATCGCTTGTG ACTCAGCGAT TTCTATACAT ACAGATGGAA 
TATTGTGAGA AGAGCACACT GCGTCAAACC ATTGATGCTG GCGTTTGTAA TGAACCTGAG 
AGAATGTGGA GATTATTCAG GGAAATTCTT GAAGGATTGG TTCACGTACA TGAGCAAGGC 
ATGATCCATA GAGATCTAAA ACCACAGAAT ATTTTCCTCG ATAAAAACGA CCATGTTAAG 
ATTGGTGATT TTGGTCTTGC TACTGCACAA GCTAGAGTGA CACTAGCTGA CAGCTCTGAA 
GAGATTTCAA AATCCGAAAC TGGAGACAGT GTATATTCTG GTGTGGTGGG AACTGCATTA 
TACGTGGCAC CTGAACTTAT GGTTACTGGT AAAAGATCAC TGTACAGTCA AAAGGTTGAC 
ATCTACAGTG TTGGAATCAT TTTCTTTGAA ATGTCATTTC CTGGTATCCA AACTAAGATG 
GAACGAGTCA AAGTTCTTGG GAACCTTAGA ACTTCTTCCA TAACCTTCCC ATCTGACTTT 
GATGAAGCTG CCATGCCTCG TGAAGCTGAG ATAACTCGAT GGTGTTTGGA TCACGTGCCA 
AACAAACGAC CAACTGCATC TGAACTTCTA AAGTGTAGAT TAGTTCCAAC TCCTGTACTA 
GAAGAATCCA ATGTACTTGA AGCAGTTCGT GAAATGTTAA GCAATCCAGA CTCAAAGCCG 
TATAGATTAG TTATGGATGA AGTTTTCAAC CAACCAGAAA ACCCAACTTC ATCTGTTATA 
TACGACATTG AAATGCATAA GAAACATTTT TCTGAGACAC AGAATGTCGC ACAACAGCAA 
ACATTTGACA TTATATCTTC CCTACTACGT TGCCATGGAG CTGTGCGGGT CAATCCTCCG 
CTCTTGTTAC CTAAATGTGG TTCAGAGAAT GATGCAAATG AAGCTGCAGT GCTAATAGGG 
GTTGGCGGTA ATGTCGTATC ACTGCCTTAT ATTCTAAGGA TTCCACTGGC TCGTTTTATT 
CTGTGTAAAG GAATCACAAA CATTAAAACT TACGCCATTG AAAAAGTTTT CCGAGCAAGA 
AAATATGCGA GGTCCCGACC TAAGGAATTA TGGGAATGTT CGTTTGACAT CATTTCCCCC 
TCAGTGGGAG GTCTCGCTCC CGACGCGGAA GTGATCTCTG TTGTTTCCGA GATCATCTCC 
CAATTCCCAT CTCTCTCCTC CCAGAAGTAC GAGGTTCGGA TCAACCACTT ATTGGCGCTA 
GAGTCAATAC TAACATACTG TGGTCTCGAT GAAAGCCACC ACACGGAGGC GCTCAATGCA 
CTCGCTTCAT GCAGTGGCCT CTCTAAAACA CAGCTCCACA ACTGCATCAA CAGCTTGCCT 
CTCAATGCTC AACAGGTTGC TTCCCTTCTT ACATTCATCC AAGTCCAAGG CCCGCTGTCC 
CGGGTTCAAA CCTCGCTGCG GTCGATTACC CGGAACAAGC ACTCACCCGC GTCGTCCAGA 
GCACGACAGG CGCTCCACGA ACTCGAAGCT CTTCGGAAAA ATCTTGATTC TTTAAACTTG 
ACACTACCTG TTGTGTTTGA TCTTGGTTAT TCTCATAAAC ACCACAACCA TTATAATGGC 
ATCATATTTA AAGTTTCAGC GGAAATCCAA AAGAAAAAAA GGACAGCAAT TGATGTGTTG 
GCCATCGGTG GTCGATATGA TAAACTGCTC ACAACCATGT CTGTATTCAA CATGTATGAC 
AGAGATGGGG AACAGAAGTT AACACCTAAG CATGCTGTGG GTGTTAGTAT TGCTGTGGAG 
AAAATTGTAG GATCCGTAAT GGAAGAAAAC AGTCTTTGTA GCTCAACAAG TATGAGTGGC 
AGCAATGACC CCACTGTGAC ATCACAATTA TCCCACCACG CTGCAGTTGA CGTGCTGGTT 
TGTTCCGTGG GGCGGACTCT TATGTTGCAC GAACGATTGA GGGTTGTAAG ATCTCTATGG 
GAGGCCTCGA TAGCAGCAGA AACCGTTTAT GATCGCGGGG CAATGGAATC AATGCAAGAA 
GTGCAGGAGA AAGCTCAAGA AAGAGGGATC CGCCATCTTG TATTTTTAAG CGAGAAAGAA 
AGTGGCTCCC CGTTAGTACG ATCATTGACG CACGACCCGC TGTTACGCTC TACTGAACGC 
AGAGTACCGC TGGATCAGTT GGTGGATTAT TTGTTAAAAC GATTGAAAGA AGTTGAATCA 
ACTGTATCAA TGAAATCTTC CTCATCGGGG TTCTCTGCAT CACACGAGTC CTCCACTGTT 
ACCATGACGA ACAATAGTGC TCGTCAAATC AGCATCGAGG TTGTTCCTCC CGAGAAACTT 
GCCTCACATT TAAGGAAGAG ATATGAGTCT GAAGTTATGA CCAAACTTAC AAGTCTACCT 
ATGATGTCCC ACATTCAATC TAAAGCTCAA ATTTGTGCCC TTGCGGTTGA ACTGCCATCA 
CCAGTTATGA AGAGCATGGG ATGTGCTCTT GATCTTTCTC ACTCTGAGGA AGAGTTTAAC 
TCTTCTCTTG CCCATCATCT ACACACTGTA TCCAAGTATA AAAAATACTT GTCCTATATC 
GCTGAGAGAA TTTGCGAGGC AAAATTCGAA CGAGAAATGA CTTTCATCAT TCTTTACAGC 
TACAAAGACC ATGGCTACTG TCTTTTGATA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018669176.1
CDS	107..5059
Translation	MTMTESESYQERQENELELLHSIFGEEIEDLRKLDKWKVVRPPEIILYLHPQEGMSGKKETFTKIDLKIKFTPTYPDSFPECKLENAKGLSEDAINDLESGIKNLYKSLQGEVMVLEIAQFVKEFLHTHNVPQCNSVYEEMVKNKQRQLEKAAQEEEKKKEIERRKKELKRKEIEEELARRQNEEKQIRKLMREGRDQNQSSQDHTRRLSEDIPNGNGSHGKSTGVVDLKINGKLPRIIHKGQTLDYIGRSTQKLKKIQKHIEICHLNTTSSGSNGSTVYAGMDANTGELVAVHYWNIPCPMRNSMLTDAERAKLDKYMKTINTIEQELNSLVKFSHKHLVPYLAMRYSHVESSIELHIVEKYITGASLQQKLTSRDRVGIDAVRTYSEQLLSALDCLHKKSVVHKNLKASSVFLSTKGSIKLADYSIEKRVIDLHNESRIEQPGVRFSEDRIPARNTKKGDILRLGMLILSLAQGQTIQDYPPEIPASFPLVFQNFLEKCLVVEDRLRYTTSELLDHEFIKPSVTSPILDIDLPLEQNPSPFDEDKMDDVLATSDAPRPTLSRLHSEFEKLEWLGKGGYGDVVKVKNIIDGRDYAIKRILLNPHRSLFNKKITREVKLLSRLNHENVVRYYNSWIETVECWEDDVSTNSSNPTTTTTTTNETKSNVSDRINSLGFYDNIEVLAPPPVASSVSDWSVSLEEVSRCRDDEEDDDEDVFKTIYVDEDDSESIKFDHSDEEEDEMVEESTNDGKTSRSEETTVTSSASSKSLVTQRFLYIQMEYCEKSTLRQTIDAGVCNEPERMWRLFREILEGLVHVHEQGMIHRDLKPQNIFLDKNDHVKIGDFGLATAQARVTLADSSEEISKSETGDSVYSGVVGTALYVAPELMVTGKRSLYSQKVDIYSVGIIFFEMSFPGIQTKMERVKVLGNLRTSSITFPSDFDEAAMPREAEITRWCLDHVPNKRPTASELLKCRLVPTPVLEESNVLEAVREMLSNPDSKPYRLVMDEVFNQPENPTSSVIYDIEMHKKHFSETQNVAQQQTFDIISSLLRCHGAVRVNPPLLLPKCGSENDANEAAVLIGVGGNVVSLPYILRIPLARFILCKGITNIKTYAIEKVFRARKYARSRPKELWECSFDIISPSVGGLAPDAEVISVVSEIISQFPSLSSQKYEVRINHLLALESILTYCGLDESHHTEALNALASCSGLSKTQLHNCINSLPLNAQQVASLLTFIQVQGPLSRVQTSLRSITRNKHSPASSRARQALHELEALRKNLDSLNLTLPVVFDLGYSHKHHNHYNGIIFKVSAEIQKKKRTAIDVLAIGGRYDKLLTTMSVFNMYDRDGEQKLTPKHAVGVSIAVEKIVGSVMEENSLCSSTSMSGSNDPTVTSQLSHHAAVDVLVCSVGRTLMLHERLRVVRSLWEASIAAETVYDRGAMESMQEVQEKAQERGIRHLVFLSEKESGSPLVRSLTHDPLLRSTERRVPLDQLVDYLLKRLKEVESTVSMKSSSSGFSASHESSTVTMTNNSARQISIEVVPPEKLASHLRKRYESEVMTKLTSLPMMSHIQSKAQICALAVELPSPVMKSMGCALDLSHSEEEFNSSLAHHLHTVSKYKKYLSYIAERICEAKFEREMTFIILYSYKDHGYCLLI

Target ORF information:

RefSeq Version	XM_018813631.2
Organism	Ciona intestinalis(vase tunicate)
Definition	Ciona intestinalis eIF-2-alpha kinase GCN2 (LOC100187226), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018813631.2

ORF Insert Sequence:

ATGACGATGA CGGAAAGTGA ATCCTATCAA GAAAGGCAGG AAAATGAACT TGAATTGCTT 
CACTCCATAT TTGGTGAGGA GATTGAAGAT CTGAGGAAAT TGGACAAATG GAAGGTGGTC 
CGTCCTCCAG AGATCATTCT CTATCTTCAT CCACAGGAAG GGATGAGCGG AAAGAAAGAA 
ACTTTTACCA AAATTGACCT CAAAATAAAA TTTACACCAA CTTACCCAGA CAGTTTCCCC 
GAGTGTAAAC TGGAGAATGC GAAAGGTTTA TCAGAAGATG CCATAAATGA TTTGGAATCT 
GGGATTAAAA ATCTCTACAA GTCATTACAG GGTGAAGTAA TGGTGCTCGA AATTGCTCAG 
TTTGTTAAGG AGTTCCTGCA TACACACAAT GTCCCACAAT GTAACTCGGT GTACGAGGAA 
ATGGTAAAAA ACAAACAAAG ACAGCTTGAA AAAGCTGCAC AGGAAGAGGA AAAGAAAAAG 
GAAATTGAAA GAAGAAAAAA GGAATTAAAG AGGAAAGAGA TAGAAGAAGA ACTAGCAAGG 
AGGCAAAACG AAGAAAAACA AATTCGTAAA CTTATGAGGG AGGGCAGAGA TCAGAACCAA 
TCATCTCAAG ACCACACACG CCGACTTTCT GAAGATATTC CGAACGGCAA TGGCAGTCAT 
GGGAAGTCTA CAGGAGTCGT GGATTTAAAA ATCAATGGAA AACTTCCACG AATAATACAT 
AAAGGGCAAA CATTAGACTA CATTGGAAGA TCCACACAAA AACTTAAAAA GATCCAGAAG 
CATATCGAGA TCTGCCATTT AAACACCACC AGTAGTGGTA GCAATGGATC AACAGTATAC 
GCGGGCATGG ATGCAAACAC AGGTGAACTA GTTGCAGTTC ATTACTGGAA CATACCATGT 
CCTATGAGGA ATTCAATGCT CACTGACGCA GAACGAGCTA AACTCGATAA ATATATGAAA 
ACAATTAACA CAATTGAGCA GGAGTTGAAT TCTTTGGTGA AATTCTCCCA CAAACACCTA 
GTGCCCTACC TTGCAATGAG ATATAGCCAT GTTGAAAGTA GTATTGAACT TCATATTGTG 
GAGAAATATA TAACAGGTGC AAGCCTTCAA CAAAAACTCA CAAGTAGAGA CAGAGTTGGA 
ATTGACGCTG TTAGAACTTA TTCTGAGCAA CTTTTATCTG CGCTTGACTG CCTACATAAA 
AAGTCTGTTG TACATAAGAA TCTTAAGGCG AGCAGTGTTT TCTTAAGCAC CAAGGGATCC 
ATCAAGCTTG CAGATTACAG CATAGAGAAA AGAGTAATAG ACTTACATAA TGAGTCCCGT 
ATTGAACAGC CTGGTGTTCG ATTCAGTGAA GATCGAATTC CTGCTCGAAA TACAAAGAAA 
GGAGATATTT TGCGGTTGGG TATGTTGATA CTGTCACTTG CACAAGGACA AACAATACAG 
GATTATCCTC CAGAAATACC AGCGAGCTTT CCACTGGTTT TCCAAAACTT TCTTGAGAAA 
TGTCTTGTGG TTGAAGACAG GCTTCGTTAT ACAACATCTG AACTCTTGGA TCATGAATTT 
ATCAAGCCAA GTGTCACCAG TCCTATCCTT GACATCGATC TTCCTTTGGA GCAAAATCCG 
AGTCCGTTTG ATGAAGATAA GATGGATGAT GTCCTTGCAA CATCCGATGC TCCAAGACCA 
ACTCTCTCTC GTCTCCACTC GGAGTTTGAG AAGTTAGAAT GGCTTGGGAA GGGAGGGTAT 
GGGGACGTGG TGAAGGTAAA GAACATAATA GACGGACGAG ATTACGCAAT CAAACGTATT 
CTACTGAACC CACATCGATC CCTGTTTAAT AAAAAGATAA CGAGAGAAGT GAAACTTTTG 
TCTCGGTTAA ACCATGAGAA TGTTGTCAGA TATTACAACT CGTGGATTGA AACAGTTGAA 
TGTTGGGAAG ATGATGTTTC AACAAATTCA TCTAACCCAA CAACAACAAC AACAACAACA 
AATGAAACAA AATCAAACGT ATCAGATCGT ATCAATTCAC TTGGTTTTTA TGATAATATT 
GAGGTTCTAG CGCCACCTCC TGTCGCATCT AGCGTGTCAG ATTGGAGCGT TTCATTGGAG 
GAGGTGAGTC GTTGTCGTGA CGACGAGGAA GATGATGACG AAGATGTGTT CAAGACTATC 
TATGTTGACG AGGATGACTC CGAGAGCATC AAGTTTGATC ACAGTGATGA AGAAGAAGAT 
GAGATGGTGG AAGAATCAAC TAATGATGGC AAAACGTCTC GGAGTGAAGA AACCACCGTC 
ACTAGTTCCG CGTCATCAAA ATCGCTTGTG ACTCAGCGAT TTCTATACAT ACAGATGGAA 
TATTGTGAGA AGAGCACACT GCGTCAAACC ATTGATGCTG GCGTTTGTAA TGAACCTGAG 
AGAATGTGGA GATTATTCAG GGAAATTCTT GAAGGATTGG TTCACGTACA TGAGCAAGGC 
ATGATCCATA GAGATCTAAA ACCACAGAAT ATTTTCCTCG ATAAAAACGA CCATGTTAAG 
ATTGGTGATT TTGGTCTTGC TACTGCACAA GCTAGAGTGA CACTAGCTGA CAGCTCTGAA 
GAGATTTCAA AATCCGAAAC TGGAGACAGT GTATATTCTG GTGTGGTGGG AACTGCATTA 
TACGTGGCAC CTGAACTTAT GGTTACTGGT AAAAGATCAC TGTACAGTCA AAAGGTTGAC 
ATCTACAGTG TTGGAATCAT TTTCTTTGAA ATGTCATTTC CTGGTATCCA AACTAAGATG 
GAACGAGTCA AAGTTCTTGG GAACCTTAGA ACTTCTTCCA TAACCTTCCC ATCTGACTTT 
GATGAAGCTG CCATGCCTCG TGAAGCTGAG ATAACTCGAT GGTGTTTGGA TCACGTGCCA 
AACAAACGAC CAACTGCATC TGAACTTCTA AAGTGTAGAT TAGTTCCAAC TCCTGTACTA 
GAAGAATCCA ATGTACTTGA AGCAGTTCGT GAAATGTTAA GCAATCCAGA CTCAAAGCCG 
TATAGATTAG TTATGGATGA AGTTTTCAAC CAACCAGAAA ACCCAACTTC ATCTGTTATA 
TACGACATTG AAATGCATAA GAAACATTTT TCTGAGACAC AGAATGTCGC ACAACAGCAA 
ACATTTGACA TTATATCTTC CCTACTACGT TGCCATGGAG CTGTGCGGGT CAATCCTCCG 
CTCTTGTTAC CTAAATGTGG TTCAGAGAAT GATGCAAATG AAGCTGCAGT GCTAATAGGG 
GTTGGCGGTA ATGTCGTATC ACTGCCTTAT ATTCTAAGGA TTCCACTGGC TCGTTTTATT 
CTGTGTAAAG GAATCACAAA CATTAAAACT TACGCCATTG AAAAAGTTTT CCGAGCAAGA 
AAATATGCGA GGTCCCGACC TAAGGAATTA TGGGAATGTT CGTTTGACAT CATTTCCCCC 
TCAGTGGGAG GTCTCGCTCC CGACGCGGAA GTGATCTCTG TTGTTTCCGA GATCATCTCC 
CAATTCCCAT CTCTCTCCTC CCAGAAGTAC GAGGTTCGGA TCAACCACTT ATTGGCGCTA 
GAGTCAATAC TAACATACTG TGGTCTCGAT GAAAGCCACC ACACGGAGGC GCTCAATGCA 
CTCGCTTCAT GCAGTGGCCT CTCTAAAACA CAGCTCCACA ACTGCATCAA CAGCTTGCCT 
CTCAATGCTC AACAGGTTGC TTCCCTTCTT ACATTCATCC AAGTCCAAGG CCCGCTGTCC 
CGGGTTCAAA CCTCGCTGCG GTCGATTACC CGGAACAAGC ACTCACCCGC GTCGTCCAGA 
GCACGACAGG CGCTCCACGA ACTCGAAGCT CTTCGGAAAA ATCTTGATTC TTTAAACTTG 
ACACTACCTG TTGTGTTTGA TCTTGGTTAT TCTCATAAAC ACCACAACCA TTATAATGGC 
ATCATATTTA AAGTTTCAGC GGAAATCCAA AAGAAAAAAA GGACAGCAAT TGATGTGTTG 
GCCATCGGTG GTCGATATGA TAAACTGCTC ACAACCATGT CTGTATTCAA CATGTATGAC 
AGAGATGGGG AACAGAAGTT AACACCTAAG CATGCTGTGG GTGTTAGTAT TGCTGTGGAG 
AAAATTGTAG GATCCGTAAT GGAAGAAAAC AGTCTTTGTA GCTCAACAAG TATGAGTGGC 
AGCAATGACC CCACTGTGAC ATCACAATTA TCCCACCACG CTGCAGTTGA CGTGCTGGTT 
TGTTCCGTGG GGCGGACTCT TATGTTGCAC GAACGATTGA GGGTTGTAAG ATCTCTATGG 
GAGGCCTCGA TAGCAGCAGA AACCGTTTAT GATCGCGGGG CAATGGAATC AATGCAAGAA 
GTGCAGGAGA AAGCTCAAGA AAGAGGGATC CGCCATCTTG TATTTTTAAG CGAGAAAGAA 
AGTGGCTCCC CGTTAGTACG ATCATTGACG CACGACCCGC TGTTACGCTC TACTGAACGC 
AGAGTACCGC TGGATCAGTT GGTGGATTAT TTGTTAAAAC GATTGAAAGA AGTTGAATCA 
ACTGTATCAA TGAAATCTTC CTCATCGGGG TTCTCTGCAT CACACGAGTC CTCCACTGTT 
ACCATGACGA ACAATAGTGC TCGTCAAATC AGCATCGAGG TTGTTCCTCC CGAGAAACTT 
GCCTCACATT TAAGGAAGAG ATATGAGTCT GAAGTTATGA CCAAACTTAC AAGTCTACCT 
ATGATGTCCC ACATTCAATC TAAAGCTCAA ATTTGTGCCC TTGCGGTTGA ACTGCCATCA 
CCAGTTATGA AGAGCATGGG ATGTGCTCTT GATCTTTCTC ACTCTGAGGA AGAGTTTAAC 
TCTTCTCTTG CCCATCATCT ACACACTGTA TCCAAGTATA AAAAATACTT GTCCTATATC 
GCTGAGAGAA TTTGCGAGGC AAAATTCGAA CGAGAAATGA CTTTCATCAT TCTTTACAGC 
TACAAAGACC ATGGCTACTG TCTTTTGATA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100187226 ( XM_018813632.1 ) cDNA ORF clone, Ciona intestinalis(vase tunicate) -> XP_018669177.1 Ciona intestinalis eIF-2-alpha kinase GCN2 (LOC100187226), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCi70883
Clone ID Related Accession (Same CDS sequence)	XM_018813632.1 , XM_018813632.2
Accession Version	XM_018813632.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-20
Organism	Ciona intestinalis(vase tunicate)
Product	eIF-2-alpha kinase GCN2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_020175.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ciona intestinalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGATGA CGGAAAGTGA ATCCTATCAA GAAAGGCAGG AAAATGAACT TGAATTGCTT 
CACTCCATAT TTGGTGAGGA GATTGAAGAT CTGAGGAAAT TGGACAAATG GAAGGTGGTC 
CGTCCTCCAG AGATCATTCT CTATCTTCAT CCACAGGAAG GGATGAGCGG AAAGAAAGAA 
ACTTTTACCA AAATTGACCT CAAAATAAAA TTTACACCAA CTTACCCAGA CAGTTTCCCC 
GAGTGTAAAC TGGAGAATGC GAAAGGTTTA TCAGAAGATG CCATAAATGA TTTGGAATCT 
GGGATTAAAA ATCTCTACAA GTCATTACAG GGTGAAGTAA TGGTGCTCGA AATTGCTCAG 
TTTGTTAAGG AGTTCCTGCA TACACACAAT GTCCCACAAT GTAACTCGGT GTACGAGGAA 
ATGGTAAAAA ACAAACAAAG ACAGCTTGAA AAAGCTGCAC AGGAAGAGGA AAAGAAAAAG 
GAAATTGAAA GAAGAAAAAA GGAATTAAAG AGGAAAGAGA TAGAAGAAGA ACTAGCAAGG 
AGGCAAAACG AAGAAAAACA AATTCGTAAA CTTATGAGGG AGGGCAGAGA TCAGAACCAA 
TCATCTCAAG ACCACACACG CCGACTTTCT GAAGATATTC CGAACGGCAA TGGCAGTCAT 
GGGAAGTCTA CAGGAGTCGT GGATTTAAAA ATCAATGGAA AACTTCCACG AATAATACAT 
AAAGGGCAAA CATTAGGTAG TGGTAGCAAT GGATCAACAG TATACGCGGG CATGGATGCA 
AACACAGGTG AACTAGTTGC AGTTCATTAC TGGAACATAC CATGTCCTAT GAGGAATTCA 
ATGCTCACTG ACGCAGAACG AGCTAAACTC GATAAATATA TGAAAACAAT TAACACAATT 
GAGCAGGAGT TGAATTCTTT GGTGAAATTC TCCCACAAAC ACCTAGTGCC CTACCTTGCA 
ATGAGATATA GCCATGTTGA AAGTAGTATT GAACTTCATA TTGTGGAGAA ATATATAACA 
GGTGCAAGCC TTCAACAAAA ACTCACAAGT AGAGACAGAG TTGGAATTGA CGCTGTTAGA 
ACTTATTCTG AGCAACTTTT ATCTGCGCTT GACTGCCTAC ATAAAAAGTC TGTTGTACAT 
AAGAATCTTA AGGCGAGCAG TGTTTTCTTA AGCACCAAGG GATCCATCAA GCTTGCAGAT 
TACAGCATAG AGAAAAGAGT AATAGACTTA CATAATGAGT CCCGTATTGA ACAGCCTGGT 
GTTCGATTCA GTGAAGATCG AATTCCTGCT CGAAATACAA AGAAAGGAGA TATTTTGCGG 
TTGGGTATGT TGATACTGTC ACTTGCACAA GGACAAACAA TACAGGATTA TCCTCCAGAA 
ATACCAGCGA GCTTTCCACT GGTTTTCCAA AACTTTCTTG AGAAATGTCT TGTGGTTGAA 
GACAGGCTTC GTTATACAAC ATCTGAACTC TTGGATCATG AATTTATCAA GCCAAGTGTC 
ACCAGTCCTA TCCTTGACAT CGATCTTCCT TTGGAGCAAA ATCCGAGTCC GTTTGATGAA 
GATAAGATGG ATGATGTCCT TGCAACATCC GATGCTCCAA GACCAACTCT CTCTCGTCTC 
CACTCGGAGT TTGAGAAGTT AGAATGGCTT GGGAAGGGAG GGTATGGGGA CGTGGTGAAG 
GTAAAGAACA TAATAGACGG ACGAGATTAC GCAATCAAAC GTATTCTACT GAACCCACAT 
CGATCCCTGT TTAATAAAAA GATAACGAGA GAAGTGAAAC TTTTGTCTCG GTTAAACCAT 
GAGAATGTTG TCAGATATTA CAACTCGTGG ATTGAAACAG TTGAATGTTG GGAAGATGAT 
GTTTCAACAA ATTCATCTAA CCCAACAACA ACAACAACAA CAACAAATGA AACAAAATCA 
AACGTATCAG ATCGTATCAA TTCACTTGGT TTTTATGATA ATATTGAGGT TCTAGCGCCA 
CCTCCTGTCG CATCTAGCGT GTCAGATTGG AGCGTTTCAT TGGAGGAGGT GAGTCGTTGT 
CGTGACGACG AGGAAGATGA TGACGAAGAT GTGTTCAAGA CTATCTATGT TGACGAGGAT 
GACTCCGAGA GCATCAAGTT TGATCACAGT GATGAAGAAG AAGATGAGAT GGTGGAAGAA 
TCAACTAATG ATGGCAAAAC GTCTCGGAGT GAAGAAACCA CCGTCACTAG TTCCGCGTCA 
TCAAAATCGC TTGTGACTCA GCGATTTCTA TACATACAGA TGGAATATTG TGAGAAGAGC 
ACACTGCGTC AAACCATTGA TGCTGGCGTT TGTAATGAAC CTGAGAGAAT GTGGAGATTA 
TTCAGGGAAA TTCTTGAAGG ATTGGTTCAC GTACATGAGC AAGGCATGAT CCATAGAGAT 
CTAAAACCAC AGAATATTTT CCTCGATAAA AACGACCATG TTAAGATTGG TGATTTTGGT 
CTTGCTACTG CACAAGCTAG AGTGACACTA GCTGACAGCT CTGAAGAGAT TTCAAAATCC 
GAAACTGGAG ACAGTGTATA TTCTGGTGTG GTGGGAACTG CATTATACGT GGCACCTGAA 
CTTATGGTTA CTGGTAAAAG ATCACTGTAC AGTCAAAAGG TTGACATCTA CAGTGTTGGA 
ATCATTTTCT TTGAAATGTC ATTTCCTGGT ATCCAAACTA AGATGGAACG AGTCAAAGTT 
CTTGGGAACC TTAGAACTTC TTCCATAACC TTCCCATCTG ACTTTGATGA AGCTGCCATG 
CCTCGTGAAG CTGAGATAAC TCGATGGTGT TTGGATCACG TGCCAAACAA ACGACCAACT 
GCATCTGAAC TTCTAAAGTG TAGATTAGTT CCAACTCCTG TACTAGAAGA ATCCAATGTA 
CTTGAAGCAG TTCGTGAAAT GTTAAGCAAT CCAGACTCAA AGCCGTATAG ATTAGTTATG 
GATGAAGTTT TCAACCAACC AGAAAACCCA ACTTCATCTG TTATATACGA CATTGAAATG 
CATAAGAAAC ATTTTTCTGA GACACAGAAT GTCGCACAAC AGCAAACATT TGACATTATA 
TCTTCCCTAC TACGTTGCCA TGGAGCTGTG CGGGTCAATC CTCCGCTCTT GTTACCTAAA 
TGTGGTTCAG AGAATGATGC AAATGAAGCT GCAGTGCTAA TAGGGGTTGG CGGTAATGTC 
GTATCACTGC CTTATATTCT AAGGATTCCA CTGGCTCGTT TTATTCTGTG TAAAGGAATC 
ACAAACATTA AAACTTACGC CATTGAAAAA GTTTTCCGAG CAAGAAAATA TGCGAGGTCC 
CGACCTAAGG AATTATGGGA ATGTTCGTTT GACATCATTT CCCCCTCAGT GGGAGGTCTC 
GCTCCCGACG CGGAAGTGAT CTCTGTTGTT TCCGAGATCA TCTCCCAATT CCCATCTCTC 
TCCTCCCAGA AGTACGAGGT TCGGATCAAC CACTTATTGG CGCTAGAGTC AATACTAACA 
TACTGTGGTC TCGATGAAAG CCACCACACG GAGGCGCTCA ATGCACTCGC TTCATGCAGT 
GGCCTCTCTA AAACACAGCT CCACAACTGC ATCAACAGCT TGCCTCTCAA TGCTCAACAG 
GTTGCTTCCC TTCTTACATT CATCCAAGTC CAAGGCCCGC TGTCCCGGGT TCAAACCTCG 
CTGCGGTCGA TTACCCGGAA CAAGCACTCA CCCGCGTCGT CCAGAGCACG ACAGGCGCTC 
CACGAACTCG AAGCTCTTCG GAAAAATCTT GATTCTTTAA ACTTGACACT ACCTGTTGTG 
TTTGATCTTG GTTATTCTCA TAAACACCAC AACCATTATA ATGGCATCAT ATTTAAAGTT 
TCAGCGGAAA TCCAAAAGAA AAAAAGGACA GCAATTGATG TGTTGGCCAT CGGTGGTCGA 
TATGATAAAC TGCTCACAAC CATGTCTGTA TTCAACATGT ATGACAGAGA TGGGGAACAG 
AAGTTAACAC CTAAGCATGC TGTGGGTGTT AGTATTGCTG TGGAGAAAAT TGTAGGATCC 
GTAATGGAAG AAAACAATCT TCCCAGCTCA ACAAGTATGA GTGGCAGCAA CGACCCCACT 
GTGACATCAC AATTATCCCA CCACGCTGCA GTTGACGTGT TGGTTTGTTC TGTCGGGCGA 
ACTCTTCTGT TGCACGAACG ATTGAGGGTT GTAAGATCTC TATGGGAGGC CTCGATAGCA 
GCAGAAACCG TTTATGATCG CGGAGCAATG GAATCAATGC AAGAAGTGCA GGAGAAAGCT 
CAAGAAAGGG GGATCCGCCA TCTTGTATTT TTAAGCGAGA AAGAAAGTGG CTCCCCGTTA 
GTACGATCAT TGACGCACGA CCCGCTGTTA CGCTCTACTG AACGCAGAGT ACCGCTGGAT 
CAGTTGGTGG ATTATTTGTT AAAACGATTG AAAGAAGTTG AATCAACTGT ATCAATGAAA 
TCTTCCTCAT CGGGGTTCTC TGCATCACAC GAGTCCTCCA CTGTTACCAT GACGAACAAT 
AGTGCTCGTC AAATCAGCAT CGAGGTTGTT CCTCCCGAGA AACTTGCCTC ACATTTAAGG 
AAGAGATATG AGTCTGAAGT TATGACCAAA CTTACAAGTC TACCTATGAT GTCCCACATT 
CAATCTAAAG CTCAAATTTG TGCCCTTGCG GTTGAACTGC CATCACCAGT TATGAAGAGC 
ATGGGATGTG CTCTTGATCT TTCTCACTCT GAGGAAGAGT TTAACTCTTC TCTTGCCCAT 
CATCTACACA CTGTATCCAA GTATAAAAAA TACTTGTCCT ATATCGCTGA GAGAATTTGC 
GAGGCAAAAT TCGAACGAGA AATGACTTTC ATCATTCTTT ACAGCTACAA AGACCATGGC 
TACTGTCTTT TGATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018669177.1
CDS	107..4984
Translation	MTMTESESYQERQENELELLHSIFGEEIEDLRKLDKWKVVRPPEIILYLHPQEGMSGKKETFTKIDLKIKFTPTYPDSFPECKLENAKGLSEDAINDLESGIKNLYKSLQGEVMVLEIAQFVKEFLHTHNVPQCNSVYEEMVKNKQRQLEKAAQEEEKKKEIERRKKELKRKEIEEELARRQNEEKQIRKLMREGRDQNQSSQDHTRRLSEDIPNGNGSHGKSTGVVDLKINGKLPRIIHKGQTLGSGSNGSTVYAGMDANTGELVAVHYWNIPCPMRNSMLTDAERAKLDKYMKTINTIEQELNSLVKFSHKHLVPYLAMRYSHVESSIELHIVEKYITGASLQQKLTSRDRVGIDAVRTYSEQLLSALDCLHKKSVVHKNLKASSVFLSTKGSIKLADYSIEKRVIDLHNESRIEQPGVRFSEDRIPARNTKKGDILRLGMLILSLAQGQTIQDYPPEIPASFPLVFQNFLEKCLVVEDRLRYTTSELLDHEFIKPSVTSPILDIDLPLEQNPSPFDEDKMDDVLATSDAPRPTLSRLHSEFEKLEWLGKGGYGDVVKVKNIIDGRDYAIKRILLNPHRSLFNKKITREVKLLSRLNHENVVRYYNSWIETVECWEDDVSTNSSNPTTTTTTTNETKSNVSDRINSLGFYDNIEVLAPPPVASSVSDWSVSLEEVSRCRDDEEDDDEDVFKTIYVDEDDSESIKFDHSDEEEDEMVEESTNDGKTSRSEETTVTSSASSKSLVTQRFLYIQMEYCEKSTLRQTIDAGVCNEPERMWRLFREILEGLVHVHEQGMIHRDLKPQNIFLDKNDHVKIGDFGLATAQARVTLADSSEEISKSETGDSVYSGVVGTALYVAPELMVTGKRSLYSQKVDIYSVGIIFFEMSFPGIQTKMERVKVLGNLRTSSITFPSDFDEAAMPREAEITRWCLDHVPNKRPTASELLKCRLVPTPVLEESNVLEAVREMLSNPDSKPYRLVMDEVFNQPENPTSSVIYDIEMHKKHFSETQNVAQQQTFDIISSLLRCHGAVRVNPPLLLPKCGSENDANEAAVLIGVGGNVVSLPYILRIPLARFILCKGITNIKTYAIEKVFRARKYARSRPKELWECSFDIISPSVGGLAPDAEVISVVSEIISQFPSLSSQKYEVRINHLLALESILTYCGLDESHHTEALNALASCSGLSKTQLHNCINSLPLNAQQVASLLTFIQVQGPLSRVQTSLRSITRNKHSPASSRARQALHELEALRKNLDSLNLTLPVVFDLGYSHKHHNHYNGIIFKVSAEIQKKKRTAIDVLAIGGRYDKLLTTMSVFNMYDRDGEQKLTPKHAVGVSIAVEKIVGSVMEENNLPSSTSMSGSNDPTVTSQLSHHAAVDVLVCSVGRTLLLHERLRVVRSLWEASIAAETVYDRGAMESMQEVQEKAQERGIRHLVFLSEKESGSPLVRSLTHDPLLRSTERRVPLDQLVDYLLKRLKEVESTVSMKSSSSGFSASHESSTVTMTNNSARQISIEVVPPEKLASHLRKRYESEVMTKLTSLPMMSHIQSKAQICALAVELPSPVMKSMGCALDLSHSEEEFNSSLAHHLHTVSKYKKYLSYIAERICEAKFEREMTFIILYSYKDHGYCLLI

Target ORF information:

RefSeq Version	XM_018813632.1
Organism	Ciona intestinalis(vase tunicate)
Definition	Ciona intestinalis eIF-2-alpha kinase GCN2 (LOC100187226), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018813632.1

ORF Insert Sequence:

ATGACGATGA CGGAAAGTGA ATCCTATCAA GAAAGGCAGG AAAATGAACT TGAATTGCTT 
CACTCCATAT TTGGTGAGGA GATTGAAGAT CTGAGGAAAT TGGACAAATG GAAGGTGGTC 
CGTCCTCCAG AGATCATTCT CTATCTTCAT CCACAGGAAG GGATGAGCGG AAAGAAAGAA 
ACTTTTACCA AAATTGACCT CAAAATAAAA TTTACACCAA CTTACCCAGA CAGTTTCCCC 
GAGTGTAAAC TGGAGAATGC GAAAGGTTTA TCAGAAGATG CCATAAATGA TTTGGAATCT 
GGGATTAAAA ATCTCTACAA GTCATTACAG GGTGAAGTAA TGGTGCTCGA AATTGCTCAG 
TTTGTTAAGG AGTTCCTGCA TACACACAAT GTCCCACAAT GTAACTCGGT GTACGAGGAA 
ATGGTAAAAA ACAAACAAAG ACAGCTTGAA AAAGCTGCAC AGGAAGAGGA AAAGAAAAAG 
GAAATTGAAA GAAGAAAAAA GGAATTAAAG AGGAAAGAGA TAGAAGAAGA ACTAGCAAGG 
AGGCAAAACG AAGAAAAACA AATTCGTAAA CTTATGAGGG AGGGCAGAGA TCAGAACCAA 
TCATCTCAAG ACCACACACG CCGACTTTCT GAAGATATTC CGAACGGCAA TGGCAGTCAT 
GGGAAGTCTA CAGGAGTCGT GGATTTAAAA ATCAATGGAA AACTTCCACG AATAATACAT 
AAAGGGCAAA CATTAGGTAG TGGTAGCAAT GGATCAACAG TATACGCGGG CATGGATGCA 
AACACAGGTG AACTAGTTGC AGTTCATTAC TGGAACATAC CATGTCCTAT GAGGAATTCA 
ATGCTCACTG ACGCAGAACG AGCTAAACTC GATAAATATA TGAAAACAAT TAACACAATT 
GAGCAGGAGT TGAATTCTTT GGTGAAATTC TCCCACAAAC ACCTAGTGCC CTACCTTGCA 
ATGAGATATA GCCATGTTGA AAGTAGTATT GAACTTCATA TTGTGGAGAA ATATATAACA 
GGTGCAAGCC TTCAACAAAA ACTCACAAGT AGAGACAGAG TTGGAATTGA CGCTGTTAGA 
ACTTATTCTG AGCAACTTTT ATCTGCGCTT GACTGCCTAC ATAAAAAGTC TGTTGTACAT 
AAGAATCTTA AGGCGAGCAG TGTTTTCTTA AGCACCAAGG GATCCATCAA GCTTGCAGAT 
TACAGCATAG AGAAAAGAGT AATAGACTTA CATAATGAGT CCCGTATTGA ACAGCCTGGT 
GTTCGATTCA GTGAAGATCG AATTCCTGCT CGAAATACAA AGAAAGGAGA TATTTTGCGG 
TTGGGTATGT TGATACTGTC ACTTGCACAA GGACAAACAA TACAGGATTA TCCTCCAGAA 
ATACCAGCGA GCTTTCCACT GGTTTTCCAA AACTTTCTTG AGAAATGTCT TGTGGTTGAA 
GACAGGCTTC GTTATACAAC ATCTGAACTC TTGGATCATG AATTTATCAA GCCAAGTGTC 
ACCAGTCCTA TCCTTGACAT CGATCTTCCT TTGGAGCAAA ATCCGAGTCC GTTTGATGAA 
GATAAGATGG ATGATGTCCT TGCAACATCC GATGCTCCAA GACCAACTCT CTCTCGTCTC 
CACTCGGAGT TTGAGAAGTT AGAATGGCTT GGGAAGGGAG GGTATGGGGA CGTGGTGAAG 
GTAAAGAACA TAATAGACGG ACGAGATTAC GCAATCAAAC GTATTCTACT GAACCCACAT 
CGATCCCTGT TTAATAAAAA GATAACGAGA GAAGTGAAAC TTTTGTCTCG GTTAAACCAT 
GAGAATGTTG TCAGATATTA CAACTCGTGG ATTGAAACAG TTGAATGTTG GGAAGATGAT 
GTTTCAACAA ATTCATCTAA CCCAACAACA ACAACAACAA CAACAAATGA AACAAAATCA 
AACGTATCAG ATCGTATCAA TTCACTTGGT TTTTATGATA ATATTGAGGT TCTAGCGCCA 
CCTCCTGTCG CATCTAGCGT GTCAGATTGG AGCGTTTCAT TGGAGGAGGT GAGTCGTTGT 
CGTGACGACG AGGAAGATGA TGACGAAGAT GTGTTCAAGA CTATCTATGT TGACGAGGAT 
GACTCCGAGA GCATCAAGTT TGATCACAGT GATGAAGAAG AAGATGAGAT GGTGGAAGAA 
TCAACTAATG ATGGCAAAAC GTCTCGGAGT GAAGAAACCA CCGTCACTAG TTCCGCGTCA 
TCAAAATCGC TTGTGACTCA GCGATTTCTA TACATACAGA TGGAATATTG TGAGAAGAGC 
ACACTGCGTC AAACCATTGA TGCTGGCGTT TGTAATGAAC CTGAGAGAAT GTGGAGATTA 
TTCAGGGAAA TTCTTGAAGG ATTGGTTCAC GTACATGAGC AAGGCATGAT CCATAGAGAT 
CTAAAACCAC AGAATATTTT CCTCGATAAA AACGACCATG TTAAGATTGG TGATTTTGGT 
CTTGCTACTG CACAAGCTAG AGTGACACTA GCTGACAGCT CTGAAGAGAT TTCAAAATCC 
GAAACTGGAG ACAGTGTATA TTCTGGTGTG GTGGGAACTG CATTATACGT GGCACCTGAA 
CTTATGGTTA CTGGTAAAAG ATCACTGTAC AGTCAAAAGG TTGACATCTA CAGTGTTGGA 
ATCATTTTCT TTGAAATGTC ATTTCCTGGT ATCCAAACTA AGATGGAACG AGTCAAAGTT 
CTTGGGAACC TTAGAACTTC TTCCATAACC TTCCCATCTG ACTTTGATGA AGCTGCCATG 
CCTCGTGAAG CTGAGATAAC TCGATGGTGT TTGGATCACG TGCCAAACAA ACGACCAACT 
GCATCTGAAC TTCTAAAGTG TAGATTAGTT CCAACTCCTG TACTAGAAGA ATCCAATGTA 
CTTGAAGCAG TTCGTGAAAT GTTAAGCAAT CCAGACTCAA AGCCGTATAG ATTAGTTATG 
GATGAAGTTT TCAACCAACC AGAAAACCCA ACTTCATCTG TTATATACGA CATTGAAATG 
CATAAGAAAC ATTTTTCTGA GACACAGAAT GTCGCACAAC AGCAAACATT TGACATTATA 
TCTTCCCTAC TACGTTGCCA TGGAGCTGTG CGGGTCAATC CTCCGCTCTT GTTACCTAAA 
TGTGGTTCAG AGAATGATGC AAATGAAGCT GCAGTGCTAA TAGGGGTTGG CGGTAATGTC 
GTATCACTGC CTTATATTCT AAGGATTCCA CTGGCTCGTT TTATTCTGTG TAAAGGAATC 
ACAAACATTA AAACTTACGC CATTGAAAAA GTTTTCCGAG CAAGAAAATA TGCGAGGTCC 
CGACCTAAGG AATTATGGGA ATGTTCGTTT GACATCATTT CCCCCTCAGT GGGAGGTCTC 
GCTCCCGACG CGGAAGTGAT CTCTGTTGTT TCCGAGATCA TCTCCCAATT CCCATCTCTC 
TCCTCCCAGA AGTACGAGGT TCGGATCAAC CACTTATTGG CGCTAGAGTC AATACTAACA 
TACTGTGGTC TCGATGAAAG CCACCACACG GAGGCGCTCA ATGCACTCGC TTCATGCAGT 
GGCCTCTCTA AAACACAGCT CCACAACTGC ATCAACAGCT TGCCTCTCAA TGCTCAACAG 
GTTGCTTCCC TTCTTACATT CATCCAAGTC CAAGGCCCGC TGTCCCGGGT TCAAACCTCG 
CTGCGGTCGA TTACCCGGAA CAAGCACTCA CCCGCGTCGT CCAGAGCACG ACAGGCGCTC 
CACGAACTCG AAGCTCTTCG GAAAAATCTT GATTCTTTAA ACTTGACACT ACCTGTTGTG 
TTTGATCTTG GTTATTCTCA TAAACACCAC AACCATTATA ATGGCATCAT ATTTAAAGTT 
TCAGCGGAAA TCCAAAAGAA AAAAAGGACA GCAATTGATG TGTTGGCCAT CGGTGGTCGA 
TATGATAAAC TGCTCACAAC CATGTCTGTA TTCAACATGT ATGACAGAGA TGGGGAACAG 
AAGTTAACAC CTAAGCATGC TGTGGGTGTT AGTATTGCTG TGGAGAAAAT TGTAGGATCC 
GTAATGGAAG AAAACAATCT TCCCAGCTCA ACAAGTATGA GTGGCAGCAA CGACCCCACT 
GTGACATCAC AATTATCCCA CCACGCTGCA GTTGACGTGT TGGTTTGTTC TGTCGGGCGA 
ACTCTTCTGT TGCACGAACG ATTGAGGGTT GTAAGATCTC TATGGGAGGC CTCGATAGCA 
GCAGAAACCG TTTATGATCG CGGAGCAATG GAATCAATGC AAGAAGTGCA GGAGAAAGCT 
CAAGAAAGGG GGATCCGCCA TCTTGTATTT TTAAGCGAGA AAGAAAGTGG CTCCCCGTTA 
GTACGATCAT TGACGCACGA CCCGCTGTTA CGCTCTACTG AACGCAGAGT ACCGCTGGAT 
CAGTTGGTGG ATTATTTGTT AAAACGATTG AAAGAAGTTG AATCAACTGT ATCAATGAAA 
TCTTCCTCAT CGGGGTTCTC TGCATCACAC GAGTCCTCCA CTGTTACCAT GACGAACAAT 
AGTGCTCGTC AAATCAGCAT CGAGGTTGTT CCTCCCGAGA AACTTGCCTC ACATTTAAGG 
AAGAGATATG AGTCTGAAGT TATGACCAAA CTTACAAGTC TACCTATGAT GTCCCACATT 
CAATCTAAAG CTCAAATTTG TGCCCTTGCG GTTGAACTGC CATCACCAGT TATGAAGAGC 
ATGGGATGTG CTCTTGATCT TTCTCACTCT GAGGAAGAGT TTAACTCTTC TCTTGCCCAT 
CATCTACACA CTGTATCCAA GTATAAAAAA TACTTGTCCT ATATCGCTGA GAGAATTTGC 
GAGGCAAAAT TCGAACGAGA AATGACTTTC ATCATTCTTT ACAGCTACAA AGACCATGGC 
TACTGTCTTT TGATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100187226 ( XM_018813632.2 ) cDNA ORF clone, Ciona intestinalis(vase tunicate) -> XP_018669177.1 Ciona intestinalis eIF-2-alpha kinase GCN2 (LOC100187226), transcript variant X5, mRNA.

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CloneID	OCi70883
Clone ID Related Accession (Same CDS sequence)	XM_018813632.1 , XM_018813632.2
Accession Version	XM_018813632.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-21
Organism	Ciona intestinalis(vase tunicate)
Product	eIF-2-alpha kinase GCN2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_020175.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Oct 22, 2018 this sequence version replaced XM_018813632.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ciona intestinalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGATGA CGGAAAGTGA ATCCTATCAA GAAAGGCAGG AAAATGAACT TGAATTGCTT 
CACTCCATAT TTGGTGAGGA GATTGAAGAT CTGAGGAAAT TGGACAAATG GAAGGTGGTC 
CGTCCTCCAG AGATCATTCT CTATCTTCAT CCACAGGAAG GGATGAGCGG AAAGAAAGAA 
ACTTTTACCA AAATTGACCT CAAAATAAAA TTTACACCAA CTTACCCAGA CAGTTTCCCC 
GAGTGTAAAC TGGAGAATGC GAAAGGTTTA TCAGAAGATG CCATAAATGA TTTGGAATCT 
GGGATTAAAA ATCTCTACAA GTCATTACAG GGTGAAGTAA TGGTGCTCGA AATTGCTCAG 
TTTGTTAAGG AGTTCCTGCA TACACACAAT GTCCCACAAT GTAACTCGGT GTACGAGGAA 
ATGGTAAAAA ACAAACAAAG ACAGCTTGAA AAAGCTGCAC AGGAAGAGGA AAAGAAAAAG 
GAAATTGAAA GAAGAAAAAA GGAATTAAAG AGGAAAGAGA TAGAAGAAGA ACTAGCAAGG 
AGGCAAAACG AAGAAAAACA AATTCGTAAA CTTATGAGGG AGGGCAGAGA TCAGAACCAA 
TCATCTCAAG ACCACACACG CCGACTTTCT GAAGATATTC CGAACGGCAA TGGCAGTCAT 
GGGAAGTCTA CAGGAGTCGT GGATTTAAAA ATCAATGGAA AACTTCCACG AATAATACAT 
AAAGGGCAAA CATTAGGTAG TGGTAGCAAT GGATCAACAG TATACGCGGG CATGGATGCA 
AACACAGGTG AACTAGTTGC AGTTCATTAC TGGAACATAC CATGTCCTAT GAGGAATTCA 
ATGCTCACTG ACGCAGAACG AGCTAAACTC GATAAATATA TGAAAACAAT TAACACAATT 
GAGCAGGAGT TGAATTCTTT GGTGAAATTC TCCCACAAAC ACCTAGTGCC CTACCTTGCA 
ATGAGATATA GCCATGTTGA AAGTAGTATT GAACTTCATA TTGTGGAGAA ATATATAACA 
GGTGCAAGCC TTCAACAAAA ACTCACAAGT AGAGACAGAG TTGGAATTGA CGCTGTTAGA 
ACTTATTCTG AGCAACTTTT ATCTGCGCTT GACTGCCTAC ATAAAAAGTC TGTTGTACAT 
AAGAATCTTA AGGCGAGCAG TGTTTTCTTA AGCACCAAGG GATCCATCAA GCTTGCAGAT 
TACAGCATAG AGAAAAGAGT AATAGACTTA CATAATGAGT CCCGTATTGA ACAGCCTGGT 
GTTCGATTCA GTGAAGATCG AATTCCTGCT CGAAATACAA AGAAAGGAGA TATTTTGCGG 
TTGGGTATGT TGATACTGTC ACTTGCACAA GGACAAACAA TACAGGATTA TCCTCCAGAA 
ATACCAGCGA GCTTTCCACT GGTTTTCCAA AACTTTCTTG AGAAATGTCT TGTGGTTGAA 
GACAGGCTTC GTTATACAAC ATCTGAACTC TTGGATCATG AATTTATCAA GCCAAGTGTC 
ACCAGTCCTA TCCTTGACAT CGATCTTCCT TTGGAGCAAA ATCCGAGTCC GTTTGATGAA 
GATAAGATGG ATGATGTCCT TGCAACATCC GATGCTCCAA GACCAACTCT CTCTCGTCTC 
CACTCGGAGT TTGAGAAGTT AGAATGGCTT GGGAAGGGAG GGTATGGGGA CGTGGTGAAG 
GTAAAGAACA TAATAGACGG ACGAGATTAC GCAATCAAAC GTATTCTACT GAACCCACAT 
CGATCCCTGT TTAATAAAAA GATAACGAGA GAAGTGAAAC TTTTGTCTCG GTTAAACCAT 
GAGAATGTTG TCAGATATTA CAACTCGTGG ATTGAAACAG TTGAATGTTG GGAAGATGAT 
GTTTCAACAA ATTCATCTAA CCCAACAACA ACAACAACAA CAACAAATGA AACAAAATCA 
AACGTATCAG ATCGTATCAA TTCACTTGGT TTTTATGATA ATATTGAGGT TCTAGCGCCA 
CCTCCTGTCG CATCTAGCGT GTCAGATTGG AGCGTTTCAT TGGAGGAGGT GAGTCGTTGT 
CGTGACGACG AGGAAGATGA TGACGAAGAT GTGTTCAAGA CTATCTATGT TGACGAGGAT 
GACTCCGAGA GCATCAAGTT TGATCACAGT GATGAAGAAG AAGATGAGAT GGTGGAAGAA 
TCAACTAATG ATGGCAAAAC GTCTCGGAGT GAAGAAACCA CCGTCACTAG TTCCGCGTCA 
TCAAAATCGC TTGTGACTCA GCGATTTCTA TACATACAGA TGGAATATTG TGAGAAGAGC 
ACACTGCGTC AAACCATTGA TGCTGGCGTT TGTAATGAAC CTGAGAGAAT GTGGAGATTA 
TTCAGGGAAA TTCTTGAAGG ATTGGTTCAC GTACATGAGC AAGGCATGAT CCATAGAGAT 
CTAAAACCAC AGAATATTTT CCTCGATAAA AACGACCATG TTAAGATTGG TGATTTTGGT 
CTTGCTACTG CACAAGCTAG AGTGACACTA GCTGACAGCT CTGAAGAGAT TTCAAAATCC 
GAAACTGGAG ACAGTGTATA TTCTGGTGTG GTGGGAACTG CATTATACGT GGCACCTGAA 
CTTATGGTTA CTGGTAAAAG ATCACTGTAC AGTCAAAAGG TTGACATCTA CAGTGTTGGA 
ATCATTTTCT TTGAAATGTC ATTTCCTGGT ATCCAAACTA AGATGGAACG AGTCAAAGTT 
CTTGGGAACC TTAGAACTTC TTCCATAACC TTCCCATCTG ACTTTGATGA AGCTGCCATG 
CCTCGTGAAG CTGAGATAAC TCGATGGTGT TTGGATCACG TGCCAAACAA ACGACCAACT 
GCATCTGAAC TTCTAAAGTG TAGATTAGTT CCAACTCCTG TACTAGAAGA ATCCAATGTA 
CTTGAAGCAG TTCGTGAAAT GTTAAGCAAT CCAGACTCAA AGCCGTATAG ATTAGTTATG 
GATGAAGTTT TCAACCAACC AGAAAACCCA ACTTCATCTG TTATATACGA CATTGAAATG 
CATAAGAAAC ATTTTTCTGA GACACAGAAT GTCGCACAAC AGCAAACATT TGACATTATA 
TCTTCCCTAC TACGTTGCCA TGGAGCTGTG CGGGTCAATC CTCCGCTCTT GTTACCTAAA 
TGTGGTTCAG AGAATGATGC AAATGAAGCT GCAGTGCTAA TAGGGGTTGG CGGTAATGTC 
GTATCACTGC CTTATATTCT AAGGATTCCA CTGGCTCGTT TTATTCTGTG TAAAGGAATC 
ACAAACATTA AAACTTACGC CATTGAAAAA GTTTTCCGAG CAAGAAAATA TGCGAGGTCC 
CGACCTAAGG AATTATGGGA ATGTTCGTTT GACATCATTT CCCCCTCAGT GGGAGGTCTC 
GCTCCCGACG CGGAAGTGAT CTCTGTTGTT TCCGAGATCA TCTCCCAATT CCCATCTCTC 
TCCTCCCAGA AGTACGAGGT TCGGATCAAC CACTTATTGG CGCTAGAGTC AATACTAACA 
TACTGTGGTC TCGATGAAAG CCACCACACG GAGGCGCTCA ATGCACTCGC TTCATGCAGT 
GGCCTCTCTA AAACACAGCT CCACAACTGC ATCAACAGCT TGCCTCTCAA TGCTCAACAG 
GTTGCTTCCC TTCTTACATT CATCCAAGTC CAAGGCCCGC TGTCCCGGGT TCAAACCTCG 
CTGCGGTCGA TTACCCGGAA CAAGCACTCA CCCGCGTCGT CCAGAGCACG ACAGGCGCTC 
CACGAACTCG AAGCTCTTCG GAAAAATCTT GATTCTTTAA ACTTGACACT ACCTGTTGTG 
TTTGATCTTG GTTATTCTCA TAAACACCAC AACCATTATA ATGGCATCAT ATTTAAAGTT 
TCAGCGGAAA TCCAAAAGAA AAAAAGGACA GCAATTGATG TGTTGGCCAT CGGTGGTCGA 
TATGATAAAC TGCTCACAAC CATGTCTGTA TTCAACATGT ATGACAGAGA TGGGGAACAG 
AAGTTAACAC CTAAGCATGC TGTGGGTGTT AGTATTGCTG TGGAGAAAAT TGTAGGATCC 
GTAATGGAAG AAAACAATCT TCCCAGCTCA ACAAGTATGA GTGGCAGCAA CGACCCCACT 
GTGACATCAC AATTATCCCA CCACGCTGCA GTTGACGTGT TGGTTTGTTC TGTCGGGCGA 
ACTCTTCTGT TGCACGAACG ATTGAGGGTT GTAAGATCTC TATGGGAGGC CTCGATAGCA 
GCAGAAACCG TTTATGATCG CGGAGCAATG GAATCAATGC AAGAAGTGCA GGAGAAAGCT 
CAAGAAAGGG GGATCCGCCA TCTTGTATTT TTAAGCGAGA AAGAAAGTGG CTCCCCGTTA 
GTACGATCAT TGACGCACGA CCCGCTGTTA CGCTCTACTG AACGCAGAGT ACCGCTGGAT 
CAGTTGGTGG ATTATTTGTT AAAACGATTG AAAGAAGTTG AATCAACTGT ATCAATGAAA 
TCTTCCTCAT CGGGGTTCTC TGCATCACAC GAGTCCTCCA CTGTTACCAT GACGAACAAT 
AGTGCTCGTC AAATCAGCAT CGAGGTTGTT CCTCCCGAGA AACTTGCCTC ACATTTAAGG 
AAGAGATATG AGTCTGAAGT TATGACCAAA CTTACAAGTC TACCTATGAT GTCCCACATT 
CAATCTAAAG CTCAAATTTG TGCCCTTGCG GTTGAACTGC CATCACCAGT TATGAAGAGC 
ATGGGATGTG CTCTTGATCT TTCTCACTCT GAGGAAGAGT TTAACTCTTC TCTTGCCCAT 
CATCTACACA CTGTATCCAA GTATAAAAAA TACTTGTCCT ATATCGCTGA GAGAATTTGC 
GAGGCAAAAT TCGAACGAGA AATGACTTTC ATCATTCTTT ACAGCTACAA AGACCATGGC 
TACTGTCTTT TGATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018669177.1
CDS	107..4984
Translation	MTMTESESYQERQENELELLHSIFGEEIEDLRKLDKWKVVRPPEIILYLHPQEGMSGKKETFTKIDLKIKFTPTYPDSFPECKLENAKGLSEDAINDLESGIKNLYKSLQGEVMVLEIAQFVKEFLHTHNVPQCNSVYEEMVKNKQRQLEKAAQEEEKKKEIERRKKELKRKEIEEELARRQNEEKQIRKLMREGRDQNQSSQDHTRRLSEDIPNGNGSHGKSTGVVDLKINGKLPRIIHKGQTLGSGSNGSTVYAGMDANTGELVAVHYWNIPCPMRNSMLTDAERAKLDKYMKTINTIEQELNSLVKFSHKHLVPYLAMRYSHVESSIELHIVEKYITGASLQQKLTSRDRVGIDAVRTYSEQLLSALDCLHKKSVVHKNLKASSVFLSTKGSIKLADYSIEKRVIDLHNESRIEQPGVRFSEDRIPARNTKKGDILRLGMLILSLAQGQTIQDYPPEIPASFPLVFQNFLEKCLVVEDRLRYTTSELLDHEFIKPSVTSPILDIDLPLEQNPSPFDEDKMDDVLATSDAPRPTLSRLHSEFEKLEWLGKGGYGDVVKVKNIIDGRDYAIKRILLNPHRSLFNKKITREVKLLSRLNHENVVRYYNSWIETVECWEDDVSTNSSNPTTTTTTTNETKSNVSDRINSLGFYDNIEVLAPPPVASSVSDWSVSLEEVSRCRDDEEDDDEDVFKTIYVDEDDSESIKFDHSDEEEDEMVEESTNDGKTSRSEETTVTSSASSKSLVTQRFLYIQMEYCEKSTLRQTIDAGVCNEPERMWRLFREILEGLVHVHEQGMIHRDLKPQNIFLDKNDHVKIGDFGLATAQARVTLADSSEEISKSETGDSVYSGVVGTALYVAPELMVTGKRSLYSQKVDIYSVGIIFFEMSFPGIQTKMERVKVLGNLRTSSITFPSDFDEAAMPREAEITRWCLDHVPNKRPTASELLKCRLVPTPVLEESNVLEAVREMLSNPDSKPYRLVMDEVFNQPENPTSSVIYDIEMHKKHFSETQNVAQQQTFDIISSLLRCHGAVRVNPPLLLPKCGSENDANEAAVLIGVGGNVVSLPYILRIPLARFILCKGITNIKTYAIEKVFRARKYARSRPKELWECSFDIISPSVGGLAPDAEVISVVSEIISQFPSLSSQKYEVRINHLLALESILTYCGLDESHHTEALNALASCSGLSKTQLHNCINSLPLNAQQVASLLTFIQVQGPLSRVQTSLRSITRNKHSPASSRARQALHELEALRKNLDSLNLTLPVVFDLGYSHKHHNHYNGIIFKVSAEIQKKKRTAIDVLAIGGRYDKLLTTMSVFNMYDRDGEQKLTPKHAVGVSIAVEKIVGSVMEENNLPSSTSMSGSNDPTVTSQLSHHAAVDVLVCSVGRTLLLHERLRVVRSLWEASIAAETVYDRGAMESMQEVQEKAQERGIRHLVFLSEKESGSPLVRSLTHDPLLRSTERRVPLDQLVDYLLKRLKEVESTVSMKSSSSGFSASHESSTVTMTNNSARQISIEVVPPEKLASHLRKRYESEVMTKLTSLPMMSHIQSKAQICALAVELPSPVMKSMGCALDLSHSEEEFNSSLAHHLHTVSKYKKYLSYIAERICEAKFEREMTFIILYSYKDHGYCLLI

Target ORF information:

RefSeq Version	XM_018813632.2
Organism	Ciona intestinalis(vase tunicate)
Definition	Ciona intestinalis eIF-2-alpha kinase GCN2 (LOC100187226), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018813632.2

ORF Insert Sequence:

ATGACGATGA CGGAAAGTGA ATCCTATCAA GAAAGGCAGG AAAATGAACT TGAATTGCTT 
CACTCCATAT TTGGTGAGGA GATTGAAGAT CTGAGGAAAT TGGACAAATG GAAGGTGGTC 
CGTCCTCCAG AGATCATTCT CTATCTTCAT CCACAGGAAG GGATGAGCGG AAAGAAAGAA 
ACTTTTACCA AAATTGACCT CAAAATAAAA TTTACACCAA CTTACCCAGA CAGTTTCCCC 
GAGTGTAAAC TGGAGAATGC GAAAGGTTTA TCAGAAGATG CCATAAATGA TTTGGAATCT 
GGGATTAAAA ATCTCTACAA GTCATTACAG GGTGAAGTAA TGGTGCTCGA AATTGCTCAG 
TTTGTTAAGG AGTTCCTGCA TACACACAAT GTCCCACAAT GTAACTCGGT GTACGAGGAA 
ATGGTAAAAA ACAAACAAAG ACAGCTTGAA AAAGCTGCAC AGGAAGAGGA AAAGAAAAAG 
GAAATTGAAA GAAGAAAAAA GGAATTAAAG AGGAAAGAGA TAGAAGAAGA ACTAGCAAGG 
AGGCAAAACG AAGAAAAACA AATTCGTAAA CTTATGAGGG AGGGCAGAGA TCAGAACCAA 
TCATCTCAAG ACCACACACG CCGACTTTCT GAAGATATTC CGAACGGCAA TGGCAGTCAT 
GGGAAGTCTA CAGGAGTCGT GGATTTAAAA ATCAATGGAA AACTTCCACG AATAATACAT 
AAAGGGCAAA CATTAGGTAG TGGTAGCAAT GGATCAACAG TATACGCGGG CATGGATGCA 
AACACAGGTG AACTAGTTGC AGTTCATTAC TGGAACATAC CATGTCCTAT GAGGAATTCA 
ATGCTCACTG ACGCAGAACG AGCTAAACTC GATAAATATA TGAAAACAAT TAACACAATT 
GAGCAGGAGT TGAATTCTTT GGTGAAATTC TCCCACAAAC ACCTAGTGCC CTACCTTGCA 
ATGAGATATA GCCATGTTGA AAGTAGTATT GAACTTCATA TTGTGGAGAA ATATATAACA 
GGTGCAAGCC TTCAACAAAA ACTCACAAGT AGAGACAGAG TTGGAATTGA CGCTGTTAGA 
ACTTATTCTG AGCAACTTTT ATCTGCGCTT GACTGCCTAC ATAAAAAGTC TGTTGTACAT 
AAGAATCTTA AGGCGAGCAG TGTTTTCTTA AGCACCAAGG GATCCATCAA GCTTGCAGAT 
TACAGCATAG AGAAAAGAGT AATAGACTTA CATAATGAGT CCCGTATTGA ACAGCCTGGT 
GTTCGATTCA GTGAAGATCG AATTCCTGCT CGAAATACAA AGAAAGGAGA TATTTTGCGG 
TTGGGTATGT TGATACTGTC ACTTGCACAA GGACAAACAA TACAGGATTA TCCTCCAGAA 
ATACCAGCGA GCTTTCCACT GGTTTTCCAA AACTTTCTTG AGAAATGTCT TGTGGTTGAA 
GACAGGCTTC GTTATACAAC ATCTGAACTC TTGGATCATG AATTTATCAA GCCAAGTGTC 
ACCAGTCCTA TCCTTGACAT CGATCTTCCT TTGGAGCAAA ATCCGAGTCC GTTTGATGAA 
GATAAGATGG ATGATGTCCT TGCAACATCC GATGCTCCAA GACCAACTCT CTCTCGTCTC 
CACTCGGAGT TTGAGAAGTT AGAATGGCTT GGGAAGGGAG GGTATGGGGA CGTGGTGAAG 
GTAAAGAACA TAATAGACGG ACGAGATTAC GCAATCAAAC GTATTCTACT GAACCCACAT 
CGATCCCTGT TTAATAAAAA GATAACGAGA GAAGTGAAAC TTTTGTCTCG GTTAAACCAT 
GAGAATGTTG TCAGATATTA CAACTCGTGG ATTGAAACAG TTGAATGTTG GGAAGATGAT 
GTTTCAACAA ATTCATCTAA CCCAACAACA ACAACAACAA CAACAAATGA AACAAAATCA 
AACGTATCAG ATCGTATCAA TTCACTTGGT TTTTATGATA ATATTGAGGT TCTAGCGCCA 
CCTCCTGTCG CATCTAGCGT GTCAGATTGG AGCGTTTCAT TGGAGGAGGT GAGTCGTTGT 
CGTGACGACG AGGAAGATGA TGACGAAGAT GTGTTCAAGA CTATCTATGT TGACGAGGAT 
GACTCCGAGA GCATCAAGTT TGATCACAGT GATGAAGAAG AAGATGAGAT GGTGGAAGAA 
TCAACTAATG ATGGCAAAAC GTCTCGGAGT GAAGAAACCA CCGTCACTAG TTCCGCGTCA 
TCAAAATCGC TTGTGACTCA GCGATTTCTA TACATACAGA TGGAATATTG TGAGAAGAGC 
ACACTGCGTC AAACCATTGA TGCTGGCGTT TGTAATGAAC CTGAGAGAAT GTGGAGATTA 
TTCAGGGAAA TTCTTGAAGG ATTGGTTCAC GTACATGAGC AAGGCATGAT CCATAGAGAT 
CTAAAACCAC AGAATATTTT CCTCGATAAA AACGACCATG TTAAGATTGG TGATTTTGGT 
CTTGCTACTG CACAAGCTAG AGTGACACTA GCTGACAGCT CTGAAGAGAT TTCAAAATCC 
GAAACTGGAG ACAGTGTATA TTCTGGTGTG GTGGGAACTG CATTATACGT GGCACCTGAA 
CTTATGGTTA CTGGTAAAAG ATCACTGTAC AGTCAAAAGG TTGACATCTA CAGTGTTGGA 
ATCATTTTCT TTGAAATGTC ATTTCCTGGT ATCCAAACTA AGATGGAACG AGTCAAAGTT 
CTTGGGAACC TTAGAACTTC TTCCATAACC TTCCCATCTG ACTTTGATGA AGCTGCCATG 
CCTCGTGAAG CTGAGATAAC TCGATGGTGT TTGGATCACG TGCCAAACAA ACGACCAACT 
GCATCTGAAC TTCTAAAGTG TAGATTAGTT CCAACTCCTG TACTAGAAGA ATCCAATGTA 
CTTGAAGCAG TTCGTGAAAT GTTAAGCAAT CCAGACTCAA AGCCGTATAG ATTAGTTATG 
GATGAAGTTT TCAACCAACC AGAAAACCCA ACTTCATCTG TTATATACGA CATTGAAATG 
CATAAGAAAC ATTTTTCTGA GACACAGAAT GTCGCACAAC AGCAAACATT TGACATTATA 
TCTTCCCTAC TACGTTGCCA TGGAGCTGTG CGGGTCAATC CTCCGCTCTT GTTACCTAAA 
TGTGGTTCAG AGAATGATGC AAATGAAGCT GCAGTGCTAA TAGGGGTTGG CGGTAATGTC 
GTATCACTGC CTTATATTCT AAGGATTCCA CTGGCTCGTT TTATTCTGTG TAAAGGAATC 
ACAAACATTA AAACTTACGC CATTGAAAAA GTTTTCCGAG CAAGAAAATA TGCGAGGTCC 
CGACCTAAGG AATTATGGGA ATGTTCGTTT GACATCATTT CCCCCTCAGT GGGAGGTCTC 
GCTCCCGACG CGGAAGTGAT CTCTGTTGTT TCCGAGATCA TCTCCCAATT CCCATCTCTC 
TCCTCCCAGA AGTACGAGGT TCGGATCAAC CACTTATTGG CGCTAGAGTC AATACTAACA 
TACTGTGGTC TCGATGAAAG CCACCACACG GAGGCGCTCA ATGCACTCGC TTCATGCAGT 
GGCCTCTCTA AAACACAGCT CCACAACTGC ATCAACAGCT TGCCTCTCAA TGCTCAACAG 
GTTGCTTCCC TTCTTACATT CATCCAAGTC CAAGGCCCGC TGTCCCGGGT TCAAACCTCG 
CTGCGGTCGA TTACCCGGAA CAAGCACTCA CCCGCGTCGT CCAGAGCACG ACAGGCGCTC 
CACGAACTCG AAGCTCTTCG GAAAAATCTT GATTCTTTAA ACTTGACACT ACCTGTTGTG 
TTTGATCTTG GTTATTCTCA TAAACACCAC AACCATTATA ATGGCATCAT ATTTAAAGTT 
TCAGCGGAAA TCCAAAAGAA AAAAAGGACA GCAATTGATG TGTTGGCCAT CGGTGGTCGA 
TATGATAAAC TGCTCACAAC CATGTCTGTA TTCAACATGT ATGACAGAGA TGGGGAACAG 
AAGTTAACAC CTAAGCATGC TGTGGGTGTT AGTATTGCTG TGGAGAAAAT TGTAGGATCC 
GTAATGGAAG AAAACAATCT TCCCAGCTCA ACAAGTATGA GTGGCAGCAA CGACCCCACT 
GTGACATCAC AATTATCCCA CCACGCTGCA GTTGACGTGT TGGTTTGTTC TGTCGGGCGA 
ACTCTTCTGT TGCACGAACG ATTGAGGGTT GTAAGATCTC TATGGGAGGC CTCGATAGCA 
GCAGAAACCG TTTATGATCG CGGAGCAATG GAATCAATGC AAGAAGTGCA GGAGAAAGCT 
CAAGAAAGGG GGATCCGCCA TCTTGTATTT TTAAGCGAGA AAGAAAGTGG CTCCCCGTTA 
GTACGATCAT TGACGCACGA CCCGCTGTTA CGCTCTACTG AACGCAGAGT ACCGCTGGAT 
CAGTTGGTGG ATTATTTGTT AAAACGATTG AAAGAAGTTG AATCAACTGT ATCAATGAAA 
TCTTCCTCAT CGGGGTTCTC TGCATCACAC GAGTCCTCCA CTGTTACCAT GACGAACAAT 
AGTGCTCGTC AAATCAGCAT CGAGGTTGTT CCTCCCGAGA AACTTGCCTC ACATTTAAGG 
AAGAGATATG AGTCTGAAGT TATGACCAAA CTTACAAGTC TACCTATGAT GTCCCACATT 
CAATCTAAAG CTCAAATTTG TGCCCTTGCG GTTGAACTGC CATCACCAGT TATGAAGAGC 
ATGGGATGTG CTCTTGATCT TTCTCACTCT GAGGAAGAGT TTAACTCTTC TCTTGCCCAT 
CATCTACACA CTGTATCCAA GTATAAAAAA TACTTGTCCT ATATCGCTGA GAGAATTTGC 
GAGGCAAAAT TCGAACGAGA AATGACTTTC ATCATTCTTT ACAGCTACAA AGACCATGGC 
TACTGTCTTT TGATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	LOC100187226
Entrez Gene ID	100187226
Full Name	eIF-2-alpha kinase GCN2
Synonyms	eif2ak4
Gene Type	protein-coding
Organism	Ciona intestinalis(vase tunicate)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

LOC100187226 cDNA ORF clone, Ciona intestinalis(vase tunicate)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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