NCKAP1 cDNA ORF clone, Chlamydotis macqueenii(Macqueen's bustard)

Gene
Clones
gRNAs

Summary

Gene Symbol	NCKAP1
Entrez Gene ID	104486063
Full Name	NCK associated protein 1
Gene Type	protein-coding
Organism	Chlamydotis macqueenii(Macqueen's bustard)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010128614.1	XP_010126916.1	nck-associated protein 1

Pathways from BioSystems
Gene Ontology

The following NCKAP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCKAP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCh199814	XM_010128614.1 Latest version!	Chlamydotis macqueenii NCK-associated protein 1 (NCKAP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCKAP1 ( XM_010128614.1 ) cDNA ORF clone, Chlamydotis macqueenii(Macqueen's bustard) -> XP_010126916.1 Chlamydotis macqueenii NCK-associated protein 1 (NCKAP1), mRNA.

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CloneID	OCh199814
Clone ID Related Accession (Same CDS sequence)	XM_010128614.1
Accession Version	XM_010128614.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-06
Organism	Chlamydotis macqueenii(Macqueen's bustard)
Product	nck-associated protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010501154.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlamydotis macqueenii Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 5% of CDS bases ##RefSeq-Attributes-END##

ATGGAGCGGG TGTTCGGAGG TGCCGTGGGT GCCGGGACCC CTCCCCGCCT TCCCGAGGCG 
AAGCGAACGG GTCTGCGCAG AGGTGAGGGG CTTCGGTGGG GCAGGAAGCC GCCGGTCTCG 
TCGATTCCAA AACTACTGTT TAAGATTCTC GTGGCTTCCT CCATTTACAA TGATGTTGAA 
ATTCAAGTTA CTAGCGATAA TCACACACAA GGACAAGTTT GGAAGGCATG CGGTGACCCC 
AAAGCCAAAC CATCCTATCT TATTGACAAA AACCTGGAAT CTGCTGTCAA ATTCATAGTC 
AGGAAGTTCC CAGCAGTAGA AACACGCAAC AACAATCAAC AGCTTGCTCA GCTCCAGAAA 
GAAAAATCGG AGATTTTGAA GAACCTGGCA TTATACTACT TCACGTTTGT TGATGTTATG 
GAATTTAAGG ACCATGTTTG TGAGCTGCTG AACACTATTG ATGTTTGCCA AGTCTTCTTT 
GATATTACTG TAAACTTTGA TTTAACAAAG AACTATCTAG ATTTGATTAT AACCTACACC 
ACACTAATGA TATTGCTGTC TCGAATTGAA GAAAGGAAAG CTATCATTGG ATTGTATAAC 
TATGCCCATG AAATGACACA CGGAGCAAGT GACAGAGAAT ATCCACGCCT TGGCCAGATG 
ATTGTGGATT ATGAAAATCC TTTAAAGAAA ATGATGGAGG AGTTTGTACC TCACAGTAAA 
TCCCTTTCAG ATGCTCTGAT TTCACTCCAG ATGGTCTATC CTCGACGAAA TCTCTCAGCT 
GACCAGTGGA GAAACGCACA ACTACTGAGC CTTATCAGTG CCCCCAGTAC AATGCTTAAT 
CCTGCACAGT CTGACACAAT GCCATGTGAA TACCTCTCTC TGGATGCAAT GGAAAAATGG 
ATCATTTTTG GATTTATTTT GTGCCATGGA ATCCTAAATA GTGATGCTAC AGCTTTGAAC 
CTTTGGAAGC TTGCACTTCA AAGCAGCTCT TGCTTGGCTC TGTTTCGAGA TGAAGTATTC 
CATATTCACA AAGCTGCAGA GGACTTGTTT GTAAACATAC GAGGCTACAA CAAGCGTATT 
AATGACATCA GAGAATGCAA AGAAGCTGCT GTGTCACATG CTGGTTCAAT ACATAGAGAG 
AGGCGCAAGT TTTTACGTTC TGCACTCAAA GAACTGGCTA CTGTCCTTTC TGATCAGCCA 
GGCCTGTTGG GTCCCAAGGC ACTTTTTGTA TTTATGGCAT TATCCTTTGC TCGTGATGAA 
ATTATTTGGC TGCTTCGTCA TGCAGATAAT ATGCCAAAGA AGAGCGCGGA TGATTTCATA 
GACAAACACA TAGCTGAGCT AATATTCTAC ATGGAGGAGC TGAGAGCACA CGTGCGAAAA 
TACGGACCAG TGATGCAGCG ATACTATGTA CAGTATCTCT CTGGCTTTGA TGCAGTAGTC 
TTGAATGAGC TTGTCCAGAA TCTTTCAGTG TGCCCAGAGG ATGAATCAAT CATCATGTCC 
TCTTTTGTAA ACACAATGAC TTCACTAAGT GTGAAACAAG TTGAAGATGG AGAGGTATTT 
GACTTCAGAG GAATGAGATT AGATTGGTTT AGGCTGCAGG CCTACACTAG TGTTTCTAAA 
GCTTCGCTTA GTCTTGCAGA CCATAGAGAA CTAGGAAAAA TGATGAACAC AATCATTTTC 
CACACAAAAA TGGTGGATTC TTTGGTGGAG ATGTTGGTTG AAACCTCAGA TCTTTCTATA 
TTTTGTTTTT ATAGTCGCGC TTTTGAGAAG ATGTTTCAGC AGTGTTTGGA GCTTCCCTCT 
CAATCAAGAT ACTCAATTGC ATTCCCACTG CTTTGTACTC ATTTCATGAG CTGTACCCAT 
GAACTGTGCC CAGAAGAGCG ACATCATATT GGAGATCGTA GTCTTTCCTT GTGTAACATG 
TTCCTGGATG AAATGGCTAA GCAAGCTCGA AATCTTATCA CAGACATTTG CACAGAACAG 
TGTACGCTGA GTGATCAGTT GCTGCCAAAG CATTGTGCCA AAACCATCAG TCAAGCAGTG 
AACAAAAAGT CAAAAAAACA GACTGGAAAG AAAGGAGAAC CCGAAAGGGA GAAACCAGGA 
GTGGAAAGCA TGAGGAAAAA CAGATTGGTT GTGACAAACC TGGACAAGCT GCACACTGCA 
CTTTCTGAGC TCTGCTTCTC AATCAATTAC GTACCAAACA TGGTGGTTTG GGAACACACG 
TTTACCCCAA GAGAATATTT AACGTCTCAC CTGGAAATCC GCTTTACCAA GTCAATTGTT 
GGAATGACTA TGTATAATCA AGCAACGCAA GAGATTGCAA AGCCGTCAGA GCTGCTAACC 
AGTGTAAGAG CCTATATGAC AGTCCTGCAG TCAATAGAAA ATTACGTACA GATCGACATT 
ACACGAGTAT TTAACAATGT TCTGCTTCAG CAAACCCAAC ATTTAGATAG TCATGGGGAG 
CCAACCATTA CCAGTCTGTA CACTAATTGG TATTTGGAAA CTTTGCTACG GCAAGTCAGC 
AGTGGCCACA TAGCCTATTT TCCAGCCATG AAGGCATTTG TGAATTTGCC TACAGAAAAT 
GAGTTGACGT TTAATGCTGA GGAATACTCT GACATATCAG AGATGAGAGC CCTTTCTGAA 
CTTTTGGGAC CATATGGCAT GAAGTTCCTA AGTGAAAGTC TGATGTGGCA CATTTCTTCA 
CAGGTTGCTG AGCTTAAGAA ACTTGTAGTG GAGAATGTTG AAGTTCTGAC ACAAATGAGG 
ACCAGCTTTG ACAAGCCAGA CCAGATGGCA GCACTCTTTA AAAGATTATC ATCTGTTGAC 
AGTGTTCTGA AGAGAATGAC AATTATCGGT GTTATCTTGT CTTTTCGGTC CTTGGCACAG 
GAAGCACTTA GAGATGTCTT ATCCTACCAC ATTCCTTTCC TTGTAAGTTC CATCGAGGAT 
TTTAAAGATC ACATTCCAAG AGAGACTGAC ATGAAGGTGG CAATGAATGT ATATGAACTG 
TCATCAGCTG CTGGATTGCC TTGTGAGATT GATCCTGCCT TGGTCGTAGC CCTGTCTTCC 
CAAAAATCAG AAAACATTAG TCCAGAAGAA GAATATAAAA TTGCTTGCCT TCTCATGGTG 
TTTGTGGCAG TCTCCTTGCC GACTTTGGCT AGTAATGTGA TGTCTCAGTA TAGCCCTGCC 
ATTGAAGGGC ATTGCAACAA TATTCATTGC TTGGCAAAAG CTATCAACCA GATTGCTGCA 
GCTTTATTTA CCATCCACAA GGGAAGCATT GAAGACCGTC TTAAAGAATT TTTGGCCCTT 
GCATCATCTA GCCTACTGAA GATAGGCCAG GAGACGGACA AAACTACTAC AAGGAACAGA 
GAATCTGTTT ACCTACTACT AGACATGATT GTGCAGGAGT CTCCATTCCT GACGATGGAT 
CTCCTGGAGT CTTGTTTCCC TTACGTCTTG CTGAGAAACG CATACCATGC TGTCTACAAA 
CAAAGCGTCA CATCCTCTGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010126916.1
CDS	1..3504
Translation	MERVFGGAVGAGTPPRLPEAKRTGLRRGEGLRWGRKPPVSSIPKLLFKILVASSIYNDVEIQVTSDNHTQGQVWKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNQQLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDVCQVFFDITVNFDLTKNYLDLIITYTTLMILLSRIEERKAIIGLYNYAHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISLQMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGILNSDATALNLWKLALQSSSCLALFRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKEAAVSHAGSIHRERRKFLRSALKELATVLSDQPGLLGPKALFVFMALSFARDEIIWLLRHADNMPKKSADDFIDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLSLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSIAFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYVPNMVVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQATQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSSGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRALSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVEVLTQMRTSFDKPDQMAALFKRLSSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA

Target ORF information:

RefSeq Version	XM_010128614.1
Organism	Chlamydotis macqueenii(Macqueen's bustard)
Definition	Chlamydotis macqueenii NCK-associated protein 1 (NCKAP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010128614.1

ORF Insert Sequence:

ATGGAGCGGG TGTTCGGAGG TGCCGTGGGT GCCGGGACCC CTCCCCGCCT TCCCGAGGCG 
AAGCGAACGG GTCTGCGCAG AGGTGAGGGG CTTCGGTGGG GCAGGAAGCC GCCGGTCTCG 
TCGATTCCAA AACTACTGTT TAAGATTCTC GTGGCTTCCT CCATTTACAA TGATGTTGAA 
ATTCAAGTTA CTAGCGATAA TCACACACAA GGACAAGTTT GGAAGGCATG CGGTGACCCC 
AAAGCCAAAC CATCCTATCT TATTGACAAA AACCTGGAAT CTGCTGTCAA ATTCATAGTC 
AGGAAGTTCC CAGCAGTAGA AACACGCAAC AACAATCAAC AGCTTGCTCA GCTCCAGAAA 
GAAAAATCGG AGATTTTGAA GAACCTGGCA TTATACTACT TCACGTTTGT TGATGTTATG 
GAATTTAAGG ACCATGTTTG TGAGCTGCTG AACACTATTG ATGTTTGCCA AGTCTTCTTT 
GATATTACTG TAAACTTTGA TTTAACAAAG AACTATCTAG ATTTGATTAT AACCTACACC 
ACACTAATGA TATTGCTGTC TCGAATTGAA GAAAGGAAAG CTATCATTGG ATTGTATAAC 
TATGCCCATG AAATGACACA CGGAGCAAGT GACAGAGAAT ATCCACGCCT TGGCCAGATG 
ATTGTGGATT ATGAAAATCC TTTAAAGAAA ATGATGGAGG AGTTTGTACC TCACAGTAAA 
TCCCTTTCAG ATGCTCTGAT TTCACTCCAG ATGGTCTATC CTCGACGAAA TCTCTCAGCT 
GACCAGTGGA GAAACGCACA ACTACTGAGC CTTATCAGTG CCCCCAGTAC AATGCTTAAT 
CCTGCACAGT CTGACACAAT GCCATGTGAA TACCTCTCTC TGGATGCAAT GGAAAAATGG 
ATCATTTTTG GATTTATTTT GTGCCATGGA ATCCTAAATA GTGATGCTAC AGCTTTGAAC 
CTTTGGAAGC TTGCACTTCA AAGCAGCTCT TGCTTGGCTC TGTTTCGAGA TGAAGTATTC 
CATATTCACA AAGCTGCAGA GGACTTGTTT GTAAACATAC GAGGCTACAA CAAGCGTATT 
AATGACATCA GAGAATGCAA AGAAGCTGCT GTGTCACATG CTGGTTCAAT ACATAGAGAG 
AGGCGCAAGT TTTTACGTTC TGCACTCAAA GAACTGGCTA CTGTCCTTTC TGATCAGCCA 
GGCCTGTTGG GTCCCAAGGC ACTTTTTGTA TTTATGGCAT TATCCTTTGC TCGTGATGAA 
ATTATTTGGC TGCTTCGTCA TGCAGATAAT ATGCCAAAGA AGAGCGCGGA TGATTTCATA 
GACAAACACA TAGCTGAGCT AATATTCTAC ATGGAGGAGC TGAGAGCACA CGTGCGAAAA 
TACGGACCAG TGATGCAGCG ATACTATGTA CAGTATCTCT CTGGCTTTGA TGCAGTAGTC 
TTGAATGAGC TTGTCCAGAA TCTTTCAGTG TGCCCAGAGG ATGAATCAAT CATCATGTCC 
TCTTTTGTAA ACACAATGAC TTCACTAAGT GTGAAACAAG TTGAAGATGG AGAGGTATTT 
GACTTCAGAG GAATGAGATT AGATTGGTTT AGGCTGCAGG CCTACACTAG TGTTTCTAAA 
GCTTCGCTTA GTCTTGCAGA CCATAGAGAA CTAGGAAAAA TGATGAACAC AATCATTTTC 
CACACAAAAA TGGTGGATTC TTTGGTGGAG ATGTTGGTTG AAACCTCAGA TCTTTCTATA 
TTTTGTTTTT ATAGTCGCGC TTTTGAGAAG ATGTTTCAGC AGTGTTTGGA GCTTCCCTCT 
CAATCAAGAT ACTCAATTGC ATTCCCACTG CTTTGTACTC ATTTCATGAG CTGTACCCAT 
GAACTGTGCC CAGAAGAGCG ACATCATATT GGAGATCGTA GTCTTTCCTT GTGTAACATG 
TTCCTGGATG AAATGGCTAA GCAAGCTCGA AATCTTATCA CAGACATTTG CACAGAACAG 
TGTACGCTGA GTGATCAGTT GCTGCCAAAG CATTGTGCCA AAACCATCAG TCAAGCAGTG 
AACAAAAAGT CAAAAAAACA GACTGGAAAG AAAGGAGAAC CCGAAAGGGA GAAACCAGGA 
GTGGAAAGCA TGAGGAAAAA CAGATTGGTT GTGACAAACC TGGACAAGCT GCACACTGCA 
CTTTCTGAGC TCTGCTTCTC AATCAATTAC GTACCAAACA TGGTGGTTTG GGAACACACG 
TTTACCCCAA GAGAATATTT AACGTCTCAC CTGGAAATCC GCTTTACCAA GTCAATTGTT 
GGAATGACTA TGTATAATCA AGCAACGCAA GAGATTGCAA AGCCGTCAGA GCTGCTAACC 
AGTGTAAGAG CCTATATGAC AGTCCTGCAG TCAATAGAAA ATTACGTACA GATCGACATT 
ACACGAGTAT TTAACAATGT TCTGCTTCAG CAAACCCAAC ATTTAGATAG TCATGGGGAG 
CCAACCATTA CCAGTCTGTA CACTAATTGG TATTTGGAAA CTTTGCTACG GCAAGTCAGC 
AGTGGCCACA TAGCCTATTT TCCAGCCATG AAGGCATTTG TGAATTTGCC TACAGAAAAT 
GAGTTGACGT TTAATGCTGA GGAATACTCT GACATATCAG AGATGAGAGC CCTTTCTGAA 
CTTTTGGGAC CATATGGCAT GAAGTTCCTA AGTGAAAGTC TGATGTGGCA CATTTCTTCA 
CAGGTTGCTG AGCTTAAGAA ACTTGTAGTG GAGAATGTTG AAGTTCTGAC ACAAATGAGG 
ACCAGCTTTG ACAAGCCAGA CCAGATGGCA GCACTCTTTA AAAGATTATC ATCTGTTGAC 
AGTGTTCTGA AGAGAATGAC AATTATCGGT GTTATCTTGT CTTTTCGGTC CTTGGCACAG 
GAAGCACTTA GAGATGTCTT ATCCTACCAC ATTCCTTTCC TTGTAAGTTC CATCGAGGAT 
TTTAAAGATC ACATTCCAAG AGAGACTGAC ATGAAGGTGG CAATGAATGT ATATGAACTG 
TCATCAGCTG CTGGATTGCC TTGTGAGATT GATCCTGCCT TGGTCGTAGC CCTGTCTTCC 
CAAAAATCAG AAAACATTAG TCCAGAAGAA GAATATAAAA TTGCTTGCCT TCTCATGGTG 
TTTGTGGCAG TCTCCTTGCC GACTTTGGCT AGTAATGTGA TGTCTCAGTA TAGCCCTGCC 
ATTGAAGGGC ATTGCAACAA TATTCATTGC TTGGCAAAAG CTATCAACCA GATTGCTGCA 
GCTTTATTTA CCATCCACAA GGGAAGCATT GAAGACCGTC TTAAAGAATT TTTGGCCCTT 
GCATCATCTA GCCTACTGAA GATAGGCCAG GAGACGGACA AAACTACTAC AAGGAACAGA 
GAATCTGTTT ACCTACTACT AGACATGATT GTGCAGGAGT CTCCATTCCT GACGATGGAT 
CTCCTGGAGT CTTGTTTCCC TTACGTCTTG CTGAGAAACG CATACCATGC TGTCTACAAA 
CAAAGCGTCA CATCCTCTGC CTAA