ORF » Species Summary » Chlamydomonas reinhardtii » ISA2 cDNA ORF clone

ISA2 cDNA ORF clone, Chlamydomonas reinhardtii

The following ISA2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ISA2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCh01546 XM_001691737.1
Latest version!		 Chlamydomonas reinhardtii isoamylase-type starch debranching enzyme (ISA2), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    ISA2 ( XM_001691737.1 ) cDNA ORF clone, Chlamydomonas reinhardtii -> XP_001691789.1 Chlamydomonas reinhardtii isoamylase-type starch debranching enzyme (ISA2), partial mRNA.
    Price & Availability↑
    CloneID OCh01546
    Clone ID Related Accession (Same CDS sequence) XM_001691737.1
    Accession Version XM_001691737.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2631bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-08-28
    Organism Chlamydomonas reinhardtii
    Product isoamylase-type starch debranching enzyme
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001843559). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGATACAAG GACACGTCCA GCCCTCCATC CTGTCCAGCT CCCATCCCGC GATGGGTCGC 
    GCCGGCTTCT CGCAGCTTCC CTCCTGGGTG CCGCAGGCCA GCGCAACGGC CACGCTGGAG 
    CGCGGCGCTG TGGACACCAG CCTCACCCGC TACCGCCGCC ACAGCAAGGC CGGCGCGGTA 
    GTGGATGCCA CGTGCGAGGC GCGTGACGGC AAGGTCATCG TGCGCGTGAC GGCGTCGTAT 
    GCCAACGCGC CGCACGCCGC GCCTTCCGAG CTGCAGCTGC ATTGGGGCAT GTACCGCGCC 
    AGCGGCACCA AGTGGCACCA CCCCAAGGAG GCCGTGCCGC CCGACAGCAC CAGACTGGAG 
    CAGGGCGGCG CCGGCGCCAT GCGCACGCCC ATGGCCTGGG ACGGCCGCGC CGGCCCCGGC 
    GGCGAGGGCG CCTGGGTGCT GCGGTTCGAG GTGCCGGCCA AGTTGGCGCC GCTGCACCTG 
    GCCTTCTCGC TGTACCAGCC GGCCACGGAC AAGTACGACG TGCCCGTCCG CGCCCCGCAC 
    TTTGCGGTGC CGGTGGGCAT GAGCGCCGGC TCGCCCCAGC CGCTGGGCGC CTCGGTGGTG 
    GCGGTGTCGC CGCCCGGCCC CAGCAACGGC GGCTTCAACG GCTTCAACGG CCACCACGGC 
    GGCGGCAGCG ACCCGCGAGA GACCACCTGC GCCGTGAACT TCGCGGTGTT CAGCCGCCAC 
    GCCTCCAGCC TGCAGCTGTG CCTGGTGCGG CTGGAGGGCG CGGAGCCGGA GGGCGGCGCC 
    GGAGGGCCGC TGGTGGCGCA GAGCGTGCTG GAGGTGGTGT TGGACCCGCT CACCAACCGC 
    ACCGGCGACG TGTGGCACGT GTGCGTGCAC GGGCTCAAGG ACCTGGAGAC GCTGTGTTGG 
    GCCTGGCGGG CGGACGGCGA GATCCTGTGG CAGAACGGCA ACCGCTTCCA CCCCGGCTTC 
    ATGCTGATGG ACCCGCACGC CACGCGCGCC GTGCCGGTGC TGCTGCCGCC GGGCGCGCAC 
    AAGGCCGCGC CGCGCATGGC GCCCTCGCTG GACGCGGGCG AGCCGGTGCT GCTGGGCACC 
    CTGGCGGCGT TCGTGCACGC GCCCTTCGAC TGGCAGGGCT GCCACCAGGC GGTGCGTGGC 
    GGCCAGAGCC GTGTGCGCGC GCTGGAGGAG AGCGTGGTGG TGGAGGTGGA TGTGGCGCGG 
    TTCACCACGG GCCGTGACGC AGAGGCCGCT GTGCCGCCCG AGCACCGCGG CAAATACCTG 
    GGCATCCTGG ACCGCCTCGA CTCGCTGAAG GCCGCCGGCG CCACCACGGT GCTGCTGAGC 
    CCGGTGTGCC TGTCCGCGCC CGGCCCCTCG CCCGCCGCCG GCCGCTCGCC GCTGGCGTTG 
    CTGGCGCCTG ACCCGGCCTT CGCGGTGGGC GGCCCGCTGG CGGCGGCGGC GGAGCTCAAG 
    GCCGTCATCC GCGGCCTGCA CCAGGCGGGG CTGGAGGTGC TGCTGCAGGT GGAGTTCTGC 
    GTGACGGCGG AGGGCGGCGA CGCGGGCGCG GGCCGGCTGC AGGGGCTGCG CGGCCTCGAC 
    CACGCCGTGT ACTACAGGGA CGGGCTGGAG GCGCCGGTGC TCAACTGCGG CCATCCGGTG 
    GACAAGTTCG GCAGTGTGCT GGACGCGCCG CCGCTGGCCG AGGACATTGC CGGCGACCCC 
    GTGCTGCGCG GCCTCAAGCT GGTGGCGGCC GTGTCCAACC CCGCGCTGCT GCCGCGGCTG 
    GCTGAGCGCG GCTTCCCGCA CTGGGGCGTG TGGCAGCAGC CACATGACCA CAACCACTAC 
    CGCCATGACC GCCATGACCA CACCCACAAC CACCGCGGCA TGCTGTCGGC AGTGGCCACC 
    CGCCTCACGG GCAGTGCCGA CTTGTTCGCG CCGCGCTGGG ACGCGGGCCT GCCGGGCGGT 
    CTGGCGGCGG GGCGGCGCGC GGGCTTCGGC CTCAACGCAG TGGCGCCGCT GGGCGACGTG 
    CCGCTGGGCG GCGTGATCGA CAGCGAGAAC GCGATGCGTG GCGACGCGCT GGCTCGCTCG 
    CTGCTGGTGG CGCAGTTCGT GTCCGCCGGC CAGCCGCTGC TGGCCGCAGC CAACCTGTCG 
    CGCCCCGGCG TGCCGCAGCT GCTGGCGGCG CTGGCGGCGG TGCGGCGCGG CTACCGCTCC 
    CTCATCTGCC CCGCCTCCAT CACCGCGCCT GAGCGCGAGG TGGCCTGGCA CTCGCCCTAC 
    GGCGGCGGCG AGCCCGACTG GAGCGGCTCC AACCCCGACC CCGTCGCCAA CTGCGTGCTG 
    CTGACGCTGG GCGGCGGCGC CAGCCGGCCC GGGCACATGC TGGCGGCGGG CTTCAACCCG 
    CATGGCGAGC CCATGTCCGT GTCGCTGCCG CGCCCGCCCG CCGGCGCCGT GTGGCGGCTG 
    CTGGTGGACA CGTCGCGGCC CGTGCCCACG GCGACCGCCG GCGGCGCCAG CCTGGGCGCG 
    GTGCTGCCGC CGTCGGAGCA GGCGCAGTAC ACGCTGGGGC CGTTTGGCTC GGTGCTGCTG 
    GATGCCGTGC CGCAGGCCGG CAGCGCGGCG GCGGTGGGCG GGCCGGTGGC AGCGGCGGCT 
    GCGCCTGGCT ACGGCTTGTC GCCCGCGGCG GCGGCGGCCC GGAAGCACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001691789.1
    CDS1..2631
    Translation

    Target ORF information:

    RefSeq Version XM_001691737.1
    Organism Chlamydomonas reinhardtii
    Definition Chlamydomonas reinhardtii isoamylase-type starch debranching enzyme (ISA2), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_001691737.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGATACAAG GACACGTCCA GCCCTCCATC CTGTCCAGCT CCCATCCCGC GATGGGTCGC 
    GCCGGCTTCT CGCAGCTTCC CTCCTGGGTG CCGCAGGCCA GCGCAACGGC CACGCTGGAG 
    CGCGGCGCTG TGGACACCAG CCTCACCCGC TACCGCCGCC ACAGCAAGGC CGGCGCGGTA 
    GTGGATGCCA CGTGCGAGGC GCGTGACGGC AAGGTCATCG TGCGCGTGAC GGCGTCGTAT 
    GCCAACGCGC CGCACGCCGC GCCTTCCGAG CTGCAGCTGC ATTGGGGCAT GTACCGCGCC 
    AGCGGCACCA AGTGGCACCA CCCCAAGGAG GCCGTGCCGC CCGACAGCAC CAGACTGGAG 
    CAGGGCGGCG CCGGCGCCAT GCGCACGCCC ATGGCCTGGG ACGGCCGCGC CGGCCCCGGC 
    GGCGAGGGCG CCTGGGTGCT GCGGTTCGAG GTGCCGGCCA AGTTGGCGCC GCTGCACCTG 
    GCCTTCTCGC TGTACCAGCC GGCCACGGAC AAGTACGACG TGCCCGTCCG CGCCCCGCAC 
    TTTGCGGTGC CGGTGGGCAT GAGCGCCGGC TCGCCCCAGC CGCTGGGCGC CTCGGTGGTG 
    GCGGTGTCGC CGCCCGGCCC CAGCAACGGC GGCTTCAACG GCTTCAACGG CCACCACGGC 
    GGCGGCAGCG ACCCGCGAGA GACCACCTGC GCCGTGAACT TCGCGGTGTT CAGCCGCCAC 
    GCCTCCAGCC TGCAGCTGTG CCTGGTGCGG CTGGAGGGCG CGGAGCCGGA GGGCGGCGCC 
    GGAGGGCCGC TGGTGGCGCA GAGCGTGCTG GAGGTGGTGT TGGACCCGCT CACCAACCGC 
    ACCGGCGACG TGTGGCACGT GTGCGTGCAC GGGCTCAAGG ACCTGGAGAC GCTGTGTTGG 
    GCCTGGCGGG CGGACGGCGA GATCCTGTGG CAGAACGGCA ACCGCTTCCA CCCCGGCTTC 
    ATGCTGATGG ACCCGCACGC CACGCGCGCC GTGCCGGTGC TGCTGCCGCC GGGCGCGCAC 
    AAGGCCGCGC CGCGCATGGC GCCCTCGCTG GACGCGGGCG AGCCGGTGCT GCTGGGCACC 
    CTGGCGGCGT TCGTGCACGC GCCCTTCGAC TGGCAGGGCT GCCACCAGGC GGTGCGTGGC 
    GGCCAGAGCC GTGTGCGCGC GCTGGAGGAG AGCGTGGTGG TGGAGGTGGA TGTGGCGCGG 
    TTCACCACGG GCCGTGACGC AGAGGCCGCT GTGCCGCCCG AGCACCGCGG CAAATACCTG 
    GGCATCCTGG ACCGCCTCGA CTCGCTGAAG GCCGCCGGCG CCACCACGGT GCTGCTGAGC 
    CCGGTGTGCC TGTCCGCGCC CGGCCCCTCG CCCGCCGCCG GCCGCTCGCC GCTGGCGTTG 
    CTGGCGCCTG ACCCGGCCTT CGCGGTGGGC GGCCCGCTGG CGGCGGCGGC GGAGCTCAAG 
    GCCGTCATCC GCGGCCTGCA CCAGGCGGGG CTGGAGGTGC TGCTGCAGGT GGAGTTCTGC 
    GTGACGGCGG AGGGCGGCGA CGCGGGCGCG GGCCGGCTGC AGGGGCTGCG CGGCCTCGAC 
    CACGCCGTGT ACTACAGGGA CGGGCTGGAG GCGCCGGTGC TCAACTGCGG CCATCCGGTG 
    GACAAGTTCG GCAGTGTGCT GGACGCGCCG CCGCTGGCCG AGGACATTGC CGGCGACCCC 
    GTGCTGCGCG GCCTCAAGCT GGTGGCGGCC GTGTCCAACC CCGCGCTGCT GCCGCGGCTG 
    GCTGAGCGCG GCTTCCCGCA CTGGGGCGTG TGGCAGCAGC CACATGACCA CAACCACTAC 
    CGCCATGACC GCCATGACCA CACCCACAAC CACCGCGGCA TGCTGTCGGC AGTGGCCACC 
    CGCCTCACGG GCAGTGCCGA CTTGTTCGCG CCGCGCTGGG ACGCGGGCCT GCCGGGCGGT 
    CTGGCGGCGG GGCGGCGCGC GGGCTTCGGC CTCAACGCAG TGGCGCCGCT GGGCGACGTG 
    CCGCTGGGCG GCGTGATCGA CAGCGAGAAC GCGATGCGTG GCGACGCGCT GGCTCGCTCG 
    CTGCTGGTGG CGCAGTTCGT GTCCGCCGGC CAGCCGCTGC TGGCCGCAGC CAACCTGTCG 
    CGCCCCGGCG TGCCGCAGCT GCTGGCGGCG CTGGCGGCGG TGCGGCGCGG CTACCGCTCC 
    CTCATCTGCC CCGCCTCCAT CACCGCGCCT GAGCGCGAGG TGGCCTGGCA CTCGCCCTAC 
    GGCGGCGGCG AGCCCGACTG GAGCGGCTCC AACCCCGACC CCGTCGCCAA CTGCGTGCTG 
    CTGACGCTGG GCGGCGGCGC CAGCCGGCCC GGGCACATGC TGGCGGCGGG CTTCAACCCG 
    CATGGCGAGC CCATGTCCGT GTCGCTGCCG CGCCCGCCCG CCGGCGCCGT GTGGCGGCTG 
    CTGGTGGACA CGTCGCGGCC CGTGCCCACG GCGACCGCCG GCGGCGCCAG CCTGGGCGCG 
    GTGCTGCCGC CGTCGGAGCA GGCGCAGTAC ACGCTGGGGC CGTTTGGCTC GGTGCTGCTG 
    GATGCCGTGC CGCAGGCCGG CAGCGCGGCG GCGGTGGGCG GGCCGGTGGC AGCGGCGGCT 
    GCGCCTGGCT ACGGCTTGTC GCCCGCGGCG GCGGCGGCCC GGAAGCACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The Chlamydomonas genome reveals the evolution of key animal and plant functions.
    Science (New York, N.Y.)318(5848)245-50(2007 Oct)
    Merchant SS,Prochnik SE,Vallon O,Harris EH,Karpowicz SJ,Witman GB,Terry A,Salamov A,Fritz-Laylin LK,Mar?chal-Drouard L,Marshall WF,Qu LH,Nelson DR,Sanderfoot AA,Spalding MH,Kapitonov VV,Ren Q,Ferris P,Lindquist E,Shapiro H,Lucas SM,Grimwood J,Schmutz J,Cardol P,Cerutti H,Chanfreau G,Chen CL,Cognat V,Croft MT,Dent R,Dutcher S,Fern?ndez E,Fukuzawa H,Gonz?lez-Ballester D,Gonz?lez-Halphen D,Hallmann A,Hanikenne M,Hippler M,Inwood W,Jabbari K,Kalanon M,Kuras R,Lefebvre PA,Lemaire SD,Lobanov AV,Lohr M,Manuell A,Meier I,Mets L,Mittag M,Mittelmeier T,Moroney JV,Moseley J,Napoli C,Nedelcu AM,Niyogi K,Novoselov SV,Paulsen IT,Pazour G,Purton S,Ral JP,Ria?o-Pach?n DM,Riekhof W,Rymarquis L,Schroda M,Stern D,Umen J,Willows R,Wilson N,Zimmer SL,Allmer J,Balk J,Bisova K,Chen CJ,Elias M,Gendler K,Hauser C,Lamb MR,Ledford H,Long JC,Minagawa J,Page MD,Pan J,Pootakham W,Roje S,Rose A,Stahlberg E,Terauchi AM,Yang P,Ball S,Bowler C,Dieckmann CL,Gladyshev VN,Green P,Jorgensen R,Mayfield S,Mueller-Roeber B,Rajamani S,Sayre RT,Brokstein P,Dubchak I,Goodstein D,Hornick L,Huang YW,Jhaveri J,Luo Y,Mart?nez D,Ngau WC,Otillar B,Poliakov A,Porter A,Szajkowski L,Werner G,Zhou K,Grigoriev IV,Rokhsar DS,Grossman AR


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