Top3a cDNA ORF clone, Castor canadensis(American beaver)

Gene
Clones
gRNAs

Summary

Gene Symbol	Top3a
Entrez Gene ID	109688187
Full Name	DNA topoisomerase III alpha
Gene Type	protein-coding
Organism	Castor canadensis(American beaver)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020166477.1	XP_020022066.1	DNA topoisomerase 3-alpha isoform X2
XM_020166476.1	XP_020022065.1	DNA topoisomerase 3-alpha isoform X1

Pathways from BioSystems
Gene Ontology

The following Top3a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Top3a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa283014	XM_020166477.1 Latest version!	Castor canadensis topoisomerase (DNA) III alpha (Top3a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OCa283013	XM_020166476.1 Latest version!	Castor canadensis topoisomerase (DNA) III alpha (Top3a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Top3a ( XM_020166477.1 ) cDNA ORF clone, Castor canadensis(American beaver) -> XP_020022066.1 Castor canadensis topoisomerase (DNA) III alpha (Top3a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCa283014
Clone ID Related Accession (Same CDS sequence)	XM_020166477.1
Accession Version	XM_020166477.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-14
Organism	Castor canadensis(American beaver)
Product	DNA topoisomerase 3-alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017870697.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Castor canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAATGA CTTCAGTCTC TGGACATTTG CTGGCTCATG ATTTCCAGAT GAAGTTTCGA 
AAGTGGCAGA GCTGCAACCC CCTTGTCCTC TTTGAAGCAG AAATCGAAAA ATACTGCCCA 
GACAATTTTG TAGATATCAA GAAAACTCTG GAACGAGAGA CACAGCAATG CCAGGCCCTG 
GTGATCTGGA CTGACTGTGA CAGAGAAGGC GAAAACATCG GGTTTGAAAT TATCCACGTG 
TGCAAAGCTG TGAAGCCTGG TCTCCAGGTG TTGAGAGCCC GTTTCTCTGA GATCACACCC 
CGTGCTGTCA GGATGGCCTG TGAAAACCTA GCTGAACCTG ATCAGAAAGT GAGTGATGCT 
GTGGATGTGA GGCAGGAGCT GGACCTAAGG ATTGGAGCTG CATTTACTAG GTTCCAGACC 
CTGCGTCTTC AAAAGATTTT TCCCGAGGTG CTGGCAGAGC AGCTCATCAG CTATGGCAGC 
TGCCAGTTCC CAACACTGGG GTTTGTGGTA GAGAGGTTCA AAGCCATTCA GGCTTTTGTA 
CCAGAAATCT TCCACAGAAT TAAAGTAACT CATGACCACA AAGATGGTAC TGTAGAGTTC 
AACTGGAAAA GGCATCGTCT CTTTAATCAC ACAGCTTGTC TTGTCCTCTA TCAGTTGTGT 
ATGGAGGATC CCATGGCAAC TGTGGTAGAG GTCAAGTCCA AGCCAAAGAG CAAGTGGAGG 
CCACAAGCTT TGGACACCGT GGAGCTGGAG AAACTAGCTT CTCGAAAATT GAGAATAAAT 
GCTAAAGAAA CCATGAGAAT TGCTGAGAGG CTCTACACCC AAGGGTACAT CAGCTATCCC 
CGAACAGAAA CAAACATTTT CCCCAAAGAC TTAAACCTGA TGGTGTTGGT AGAACAGCAG 
ACTCCAGATC CACACTGGGG GGCCTTTGCC CAGAGCATTC TAGAGAGGGG TGGCCCCACC 
CCACGCAATG GAAGCAAGTC TGACCAAGCT CACCCTCCCA TTCACCCCAC TAAATACACC 
AGTAACTTGC AGGGAGATGA ACGGCGACTG TATGAGTTCA TCGTTCGCCA TTTTCTGGCT 
TGCTGCTCCC AGGATGCTCA GGGGCAGGAA ACCACCGTGG AAATTGACAT TGCTCAGGAA 
CGCTTTGTGG CTCATGGCCT TGTGATTCTG GCCCGAAACT ATCTGGATGT GTATCCATAT 
GATCATTGGA GTGACAAGCT CCTCCCTGTC TATGAGCAAG GCTCCTGCTT TCAGCCCAGC 
ACCGTAGAGA TGGTGGATGG TGAGACCAGC CCACCCCAGC TGCTCACTGA GGCTGACCTC 
ATCGCCCTCA TGGAGAAGCA TGGCATCGGT ACTGATGCCA CTCACGCAGA ACATATTGAA 
ACCATCAAGG CACGGATGTA TGTGGGGCTC ACCTCAGACA AGCGGTTCCT CCCCGGGCAC 
CTGGGCATGG GACTTGTGGA AGGTTACGAT TCCATGGGCT ATGAGATGTC CAAACCTGAC 
CTCCGGGCTG AGCTGGAAGC TGATCTGAAA CTGATCTGTG AGGGCAAGAA AGACAAGCTT 
GTGGTTCTAA GACAGCAAGT GCAGAAATAT AAGCAGGTTT TCATTGAAGC AGTGGCTAAG 
GCGAAGAAAT TGGATGAGGC CTTGTCCCAG TATTTTGGGG AAGGGGCAGA GATGGCCCAA 
CAAGAAGAGA TCTACCCACC CATGCCAGAC CCCATCCGGA AGTGCCCACA ATGCAACAAG 
GACATGGTCC TCAAGACCAA GAAGAGTGGC GGGTTCTACC TCAGTTGCAC AGGTTTCCCA 
GAATGTCGAT CAGCTGTATG GCTTCCTGAC TCGGTGTTGG AGGCCAGCAA GGATGGGAGT 
GTGTGTTCAG TTTGTCAGCC ACCCCCTGTA CACAGGTTGA AATTGAAGTT TAAGCGTGGC 
AGCCTTCCCC CAACCATGCC CCTGGAGTTT GTTGCTTGCA TCGGTGGATG TGATGAAACC 
CTGAAGGAGA TCTTGGGTCT GAGATTTTCA CAGGGCCCCC CGAGAGCTAT TCAGCCTTCT 
GGCCACCTGC AGGCTAGCCA AGCCCTGAAC AGGATGGACA GTGGGCAGCA CAGCCACCCC 
CAGCCTCCTA GAAGCAGACA GACTGGACCC TCAAAGCCTG TGGCCCAGAC TCTCCTACCA 
CCCACTGCCA ATAGCGAAAG TAACTCTGTG ACCTGCAACT GTGGCCAGGA GGCCGTGCTG 
CTCACTGTCC GCAAGGAGGG CCCCAACCAG GGCCGGCAGT TCTACAAGTG CAACAGTGGC 
AGCTGCAACT TTTTCCTGTG GGCTGATGGC AACCATCCTA CAACAGGAAG ACCCCCCACC 
TCTGCACCAA GGCCTCCCAG CAGCTCACTG GGACTCCCTC CAAGTTCATG CAGCTATCTG 
GGTGGTTTCG ACAACCCTGG CAATGGTGGT GGTGGTGGTG GTGGCACATC CTGCCTGTGT 
AGCCAGCCTG CTGTCACACG GACTGTGCAG AAGGACGGGC CCAACAAAGG TCGCCAATTC 
CACACATGCT CTAAGCCACG AGAGCAACAG TGTGGCTTCT TTCAGTGGGT TGATGAGAAT 
ATAGCCCCAG GGACTTTTGT GGCCCTACCC TGGCCAGGAG GCAGAGGAAA AACCCAAGGG 
CCAGAGGCCA GAAGCAAAAG ACCCAGGGCC AGTTCCTCAG AGGCAGGGTC CACAGCAAAG 
AAGTCTCGGA AATGCAGCCT TTGCCACCAG CCTGGACATA CTCGGCCCTT CTGTCCTCAG 
AACAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020022066.1
CDS	351..3119
Translation	MVMTSVSGHLLAHDFQMKFRKWQSCNPLVLFEAEIEKYCPDNFVDIKKTLERETQQCQALVIWTDCDREGENIGFEIIHVCKAVKPGLQVLRARFSEITPRAVRMACENLAEPDQKVSDAVDVRQELDLRIGAAFTRFQTLRLQKIFPEVLAEQLISYGSCQFPTLGFVVERFKAIQAFVPEIFHRIKVTHDHKDGTVEFNWKRHRLFNHTACLVLYQLCMEDPMATVVEVKSKPKSKWRPQALDTVELEKLASRKLRINAKETMRIAERLYTQGYISYPRTETNIFPKDLNLMVLVEQQTPDPHWGAFAQSILERGGPTPRNGSKSDQAHPPIHPTKYTSNLQGDERRLYEFIVRHFLACCSQDAQGQETTVEIDIAQERFVAHGLVILARNYLDVYPYDHWSDKLLPVYEQGSCFQPSTVEMVDGETSPPQLLTEADLIALMEKHGIGTDATHAEHIETIKARMYVGLTSDKRFLPGHLGMGLVEGYDSMGYEMSKPDLRAELEADLKLICEGKKDKLVVLRQQVQKYKQVFIEAVAKAKKLDEALSQYFGEGAEMAQQEEIYPPMPDPIRKCPQCNKDMVLKTKKSGGFYLSCTGFPECRSAVWLPDSVLEASKDGSVCSVCQPPPVHRLKLKFKRGSLPPTMPLEFVACIGGCDETLKEILGLRFSQGPPRAIQPSGHLQASQALNRMDSGQHSHPQPPRSRQTGPSKPVAQTLLPPTANSESNSVTCNCGQEAVLLTVRKEGPNQGRQFYKCNSGSCNFFLWADGNHPTTGRPPTSAPRPPSSSLGLPPSSCSYLGGFDNPGNGGGGGGGTSCLCSQPAVTRTVQKDGPNKGRQFHTCSKPREQQCGFFQWVDENIAPGTFVALPWPGGRGKTQGPEARSKRPRASSSEAGSTAKKSRKCSLCHQPGHTRPFCPQNR

Target ORF information:

RefSeq Version	XM_020166477.1
Organism	Castor canadensis(American beaver)
Definition	Castor canadensis topoisomerase (DNA) III alpha (Top3a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020166477.1

ORF Insert Sequence:

ATGGTAATGA CTTCAGTCTC TGGACATTTG CTGGCTCATG ATTTCCAGAT GAAGTTTCGA 
AAGTGGCAGA GCTGCAACCC CCTTGTCCTC TTTGAAGCAG AAATCGAAAA ATACTGCCCA 
GACAATTTTG TAGATATCAA GAAAACTCTG GAACGAGAGA CACAGCAATG CCAGGCCCTG 
GTGATCTGGA CTGACTGTGA CAGAGAAGGC GAAAACATCG GGTTTGAAAT TATCCACGTG 
TGCAAAGCTG TGAAGCCTGG TCTCCAGGTG TTGAGAGCCC GTTTCTCTGA GATCACACCC 
CGTGCTGTCA GGATGGCCTG TGAAAACCTA GCTGAACCTG ATCAGAAAGT GAGTGATGCT 
GTGGATGTGA GGCAGGAGCT GGACCTAAGG ATTGGAGCTG CATTTACTAG GTTCCAGACC 
CTGCGTCTTC AAAAGATTTT TCCCGAGGTG CTGGCAGAGC AGCTCATCAG CTATGGCAGC 
TGCCAGTTCC CAACACTGGG GTTTGTGGTA GAGAGGTTCA AAGCCATTCA GGCTTTTGTA 
CCAGAAATCT TCCACAGAAT TAAAGTAACT CATGACCACA AAGATGGTAC TGTAGAGTTC 
AACTGGAAAA GGCATCGTCT CTTTAATCAC ACAGCTTGTC TTGTCCTCTA TCAGTTGTGT 
ATGGAGGATC CCATGGCAAC TGTGGTAGAG GTCAAGTCCA AGCCAAAGAG CAAGTGGAGG 
CCACAAGCTT TGGACACCGT GGAGCTGGAG AAACTAGCTT CTCGAAAATT GAGAATAAAT 
GCTAAAGAAA CCATGAGAAT TGCTGAGAGG CTCTACACCC AAGGGTACAT CAGCTATCCC 
CGAACAGAAA CAAACATTTT CCCCAAAGAC TTAAACCTGA TGGTGTTGGT AGAACAGCAG 
ACTCCAGATC CACACTGGGG GGCCTTTGCC CAGAGCATTC TAGAGAGGGG TGGCCCCACC 
CCACGCAATG GAAGCAAGTC TGACCAAGCT CACCCTCCCA TTCACCCCAC TAAATACACC 
AGTAACTTGC AGGGAGATGA ACGGCGACTG TATGAGTTCA TCGTTCGCCA TTTTCTGGCT 
TGCTGCTCCC AGGATGCTCA GGGGCAGGAA ACCACCGTGG AAATTGACAT TGCTCAGGAA 
CGCTTTGTGG CTCATGGCCT TGTGATTCTG GCCCGAAACT ATCTGGATGT GTATCCATAT 
GATCATTGGA GTGACAAGCT CCTCCCTGTC TATGAGCAAG GCTCCTGCTT TCAGCCCAGC 
ACCGTAGAGA TGGTGGATGG TGAGACCAGC CCACCCCAGC TGCTCACTGA GGCTGACCTC 
ATCGCCCTCA TGGAGAAGCA TGGCATCGGT ACTGATGCCA CTCACGCAGA ACATATTGAA 
ACCATCAAGG CACGGATGTA TGTGGGGCTC ACCTCAGACA AGCGGTTCCT CCCCGGGCAC 
CTGGGCATGG GACTTGTGGA AGGTTACGAT TCCATGGGCT ATGAGATGTC CAAACCTGAC 
CTCCGGGCTG AGCTGGAAGC TGATCTGAAA CTGATCTGTG AGGGCAAGAA AGACAAGCTT 
GTGGTTCTAA GACAGCAAGT GCAGAAATAT AAGCAGGTTT TCATTGAAGC AGTGGCTAAG 
GCGAAGAAAT TGGATGAGGC CTTGTCCCAG TATTTTGGGG AAGGGGCAGA GATGGCCCAA 
CAAGAAGAGA TCTACCCACC CATGCCAGAC CCCATCCGGA AGTGCCCACA ATGCAACAAG 
GACATGGTCC TCAAGACCAA GAAGAGTGGC GGGTTCTACC TCAGTTGCAC AGGTTTCCCA 
GAATGTCGAT CAGCTGTATG GCTTCCTGAC TCGGTGTTGG AGGCCAGCAA GGATGGGAGT 
GTGTGTTCAG TTTGTCAGCC ACCCCCTGTA CACAGGTTGA AATTGAAGTT TAAGCGTGGC 
AGCCTTCCCC CAACCATGCC CCTGGAGTTT GTTGCTTGCA TCGGTGGATG TGATGAAACC 
CTGAAGGAGA TCTTGGGTCT GAGATTTTCA CAGGGCCCCC CGAGAGCTAT TCAGCCTTCT 
GGCCACCTGC AGGCTAGCCA AGCCCTGAAC AGGATGGACA GTGGGCAGCA CAGCCACCCC 
CAGCCTCCTA GAAGCAGACA GACTGGACCC TCAAAGCCTG TGGCCCAGAC TCTCCTACCA 
CCCACTGCCA ATAGCGAAAG TAACTCTGTG ACCTGCAACT GTGGCCAGGA GGCCGTGCTG 
CTCACTGTCC GCAAGGAGGG CCCCAACCAG GGCCGGCAGT TCTACAAGTG CAACAGTGGC 
AGCTGCAACT TTTTCCTGTG GGCTGATGGC AACCATCCTA CAACAGGAAG ACCCCCCACC 
TCTGCACCAA GGCCTCCCAG CAGCTCACTG GGACTCCCTC CAAGTTCATG CAGCTATCTG 
GGTGGTTTCG ACAACCCTGG CAATGGTGGT GGTGGTGGTG GTGGCACATC CTGCCTGTGT 
AGCCAGCCTG CTGTCACACG GACTGTGCAG AAGGACGGGC CCAACAAAGG TCGCCAATTC 
CACACATGCT CTAAGCCACG AGAGCAACAG TGTGGCTTCT TTCAGTGGGT TGATGAGAAT 
ATAGCCCCAG GGACTTTTGT GGCCCTACCC TGGCCAGGAG GCAGAGGAAA AACCCAAGGG 
CCAGAGGCCA GAAGCAAAAG ACCCAGGGCC AGTTCCTCAG AGGCAGGGTC CACAGCAAAG 
AAGTCTCGGA AATGCAGCCT TTGCCACCAG CCTGGACATA CTCGGCCCTT CTGTCCTCAG 
AACAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Top3a ( XM_020166476.1 ) cDNA ORF clone, Castor canadensis(American beaver) -> XP_020022065.1 Castor canadensis topoisomerase (DNA) III alpha (Top3a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCa283013
Clone ID Related Accession (Same CDS sequence)	XM_020166476.1
Accession Version	XM_020166476.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3018bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-14
Organism	Castor canadensis(American beaver)
Product	DNA topoisomerase 3-alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017870697.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Castor canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGTCC CGGTTGCCCG CTTCGTGTTC CCGTGGCTGC GACGATCGGG AGACCGTGCC 
TTGTCCCGGG CCGCCATGGA AGTGGCTCTT CGAGGCGTGC GGAAAGTCCT CTGTGTGGCG 
GAGAAAAATG ACGCGGCCAA GGGGATCGCC GACCTGCTGT CCAACGGTCG CATGAGGCGG 
AGGGAAGGAC TTTCTAAGTT CAACAAGATC TACGAATTTG ATTATCATCT ATTTGGCCAG 
AATGTTACCA TGGTAATGAC TTCAGTCTCT GGACATTTGC TGGCTCATGA TTTCCAGATG 
AAGTTTCGAA AGTGGCAGAG CTGCAACCCC CTTGTCCTCT TTGAAGCAGA AATCGAAAAA 
TACTGCCCAG ACAATTTTGT AGATATCAAG AAAACTCTGG AACGAGAGAC ACAGCAATGC 
CAGGCCCTGG TGATCTGGAC TGACTGTGAC AGAGAAGGCG AAAACATCGG GTTTGAAATT 
ATCCACGTGT GCAAAGCTGT GAAGCCTGGT CTCCAGGTGT TGAGAGCCCG TTTCTCTGAG 
ATCACACCCC GTGCTGTCAG GATGGCCTGT GAAAACCTAG CTGAACCTGA TCAGAAAGTG 
AGTGATGCTG TGGATGTGAG GCAGGAGCTG GACCTAAGGA TTGGAGCTGC ATTTACTAGG 
TTCCAGACCC TGCGTCTTCA AAAGATTTTT CCCGAGGTGC TGGCAGAGCA GCTCATCAGC 
TATGGCAGCT GCCAGTTCCC AACACTGGGG TTTGTGGTAG AGAGGTTCAA AGCCATTCAG 
GCTTTTGTAC CAGAAATCTT CCACAGAATT AAAGTAACTC ATGACCACAA AGATGGTACT 
GTAGAGTTCA ACTGGAAAAG GCATCGTCTC TTTAATCACA CAGCTTGTCT TGTCCTCTAT 
CAGTTGTGTA TGGAGGATCC CATGGCAACT GTGGTAGAGG TCAAGTCCAA GCCAAAGAGC 
AAGTGGAGGC CACAAGCTTT GGACACCGTG GAGCTGGAGA AACTAGCTTC TCGAAAATTG 
AGAATAAATG CTAAAGAAAC CATGAGAATT GCTGAGAGGC TCTACACCCA AGGGTACATC 
AGCTATCCCC GAACAGAAAC AAACATTTTC CCCAAAGACT TAAACCTGAT GGTGTTGGTA 
GAACAGCAGA CTCCAGATCC ACACTGGGGG GCCTTTGCCC AGAGCATTCT AGAGAGGGGT 
GGCCCCACCC CACGCAATGG AAGCAAGTCT GACCAAGCTC ACCCTCCCAT TCACCCCACT 
AAATACACCA GTAACTTGCA GGGAGATGAA CGGCGACTGT ATGAGTTCAT CGTTCGCCAT 
TTTCTGGCTT GCTGCTCCCA GGATGCTCAG GGGCAGGAAA CCACCGTGGA AATTGACATT 
GCTCAGGAAC GCTTTGTGGC TCATGGCCTT GTGATTCTGG CCCGAAACTA TCTGGATGTG 
TATCCATATG ATCATTGGAG TGACAAGCTC CTCCCTGTCT ATGAGCAAGG CTCCTGCTTT 
CAGCCCAGCA CCGTAGAGAT GGTGGATGGT GAGACCAGCC CACCCCAGCT GCTCACTGAG 
GCTGACCTCA TCGCCCTCAT GGAGAAGCAT GGCATCGGTA CTGATGCCAC TCACGCAGAA 
CATATTGAAA CCATCAAGGC ACGGATGTAT GTGGGGCTCA CCTCAGACAA GCGGTTCCTC 
CCCGGGCACC TGGGCATGGG ACTTGTGGAA GGTTACGATT CCATGGGCTA TGAGATGTCC 
AAACCTGACC TCCGGGCTGA GCTGGAAGCT GATCTGAAAC TGATCTGTGA GGGCAAGAAA 
GACAAGCTTG TGGTTCTAAG ACAGCAAGTG CAGAAATATA AGCAGGTTTT CATTGAAGCA 
GTGGCTAAGG CGAAGAAATT GGATGAGGCC TTGTCCCAGT ATTTTGGGGA AGGGGCAGAG 
ATGGCCCAAC AAGAAGAGAT CTACCCACCC ATGCCAGACC CCATCCGGAA GTGCCCACAA 
TGCAACAAGG ACATGGTCCT CAAGACCAAG AAGAGTGGCG GGTTCTACCT CAGTTGCACA 
GGTTTCCCAG AATGTCGATC AGCTGTATGG CTTCCTGACT CGGTGTTGGA GGCCAGCAAG 
GATGGGAGTG TGTGTTCAGT TTGTCAGCCA CCCCCTGTAC ACAGGTTGAA ATTGAAGTTT 
AAGCGTGGCA GCCTTCCCCC AACCATGCCC CTGGAGTTTG TTGCTTGCAT CGGTGGATGT 
GATGAAACCC TGAAGGAGAT CTTGGGTCTG AGATTTTCAC AGGGCCCCCC GAGAGCTATT 
CAGCCTTCTG GCCACCTGCA GGCTAGCCAA GCCCTGAACA GGATGGACAG TGGGCAGCAC 
AGCCACCCCC AGCCTCCTAG AAGCAGACAG ACTGGACCCT CAAAGCCTGT GGCCCAGACT 
CTCCTACCAC CCACTGCCAA TAGCGAAAGT AACTCTGTGA CCTGCAACTG TGGCCAGGAG 
GCCGTGCTGC TCACTGTCCG CAAGGAGGGC CCCAACCAGG GCCGGCAGTT CTACAAGTGC 
AACAGTGGCA GCTGCAACTT TTTCCTGTGG GCTGATGGCA ACCATCCTAC AACAGGAAGA 
CCCCCCACCT CTGCACCAAG GCCTCCCAGC AGCTCACTGG GACTCCCTCC AAGTTCATGC 
AGCTATCTGG GTGGTTTCGA CAACCCTGGC AATGGTGGTG GTGGTGGTGG TGGCACATCC 
TGCCTGTGTA GCCAGCCTGC TGTCACACGG ACTGTGCAGA AGGACGGGCC CAACAAAGGT 
CGCCAATTCC ACACATGCTC TAAGCCACGA GAGCAACAGT GTGGCTTCTT TCAGTGGGTT 
GATGAGAATA TAGCCCCAGG GACTTTTGTG GCCCTACCCT GGCCAGGAGG CAGAGGAAAA 
ACCCAAGGGC CAGAGGCCAG AAGCAAAAGA CCCAGGGCCA GTTCCTCAGA GGCAGGGTCC 
ACAGCAAAGA AGTCTCGGAA ATGCAGCCTT TGCCACCAGC CTGGACATAC TCGGCCCTTC 
TGTCCTCAGA ACAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020022065.1
CDS	158..3175
Translation	MNVPVARFVFPWLRRSGDRALSRAAMEVALRGVRKVLCVAEKNDAAKGIADLLSNGRMRRREGLSKFNKIYEFDYHLFGQNVTMVMTSVSGHLLAHDFQMKFRKWQSCNPLVLFEAEIEKYCPDNFVDIKKTLERETQQCQALVIWTDCDREGENIGFEIIHVCKAVKPGLQVLRARFSEITPRAVRMACENLAEPDQKVSDAVDVRQELDLRIGAAFTRFQTLRLQKIFPEVLAEQLISYGSCQFPTLGFVVERFKAIQAFVPEIFHRIKVTHDHKDGTVEFNWKRHRLFNHTACLVLYQLCMEDPMATVVEVKSKPKSKWRPQALDTVELEKLASRKLRINAKETMRIAERLYTQGYISYPRTETNIFPKDLNLMVLVEQQTPDPHWGAFAQSILERGGPTPRNGSKSDQAHPPIHPTKYTSNLQGDERRLYEFIVRHFLACCSQDAQGQETTVEIDIAQERFVAHGLVILARNYLDVYPYDHWSDKLLPVYEQGSCFQPSTVEMVDGETSPPQLLTEADLIALMEKHGIGTDATHAEHIETIKARMYVGLTSDKRFLPGHLGMGLVEGYDSMGYEMSKPDLRAELEADLKLICEGKKDKLVVLRQQVQKYKQVFIEAVAKAKKLDEALSQYFGEGAEMAQQEEIYPPMPDPIRKCPQCNKDMVLKTKKSGGFYLSCTGFPECRSAVWLPDSVLEASKDGSVCSVCQPPPVHRLKLKFKRGSLPPTMPLEFVACIGGCDETLKEILGLRFSQGPPRAIQPSGHLQASQALNRMDSGQHSHPQPPRSRQTGPSKPVAQTLLPPTANSESNSVTCNCGQEAVLLTVRKEGPNQGRQFYKCNSGSCNFFLWADGNHPTTGRPPTSAPRPPSSSLGLPPSSCSYLGGFDNPGNGGGGGGGTSCLCSQPAVTRTVQKDGPNKGRQFHTCSKPREQQCGFFQWVDENIAPGTFVALPWPGGRGKTQGPEARSKRPRASSSEAGSTAKKSRKCSLCHQPGHTRPFCPQNR

Target ORF information:

RefSeq Version	XM_020166476.1
Organism	Castor canadensis(American beaver)
Definition	Castor canadensis topoisomerase (DNA) III alpha (Top3a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020166476.1

ORF Insert Sequence:

ATGAACGTCC CGGTTGCCCG CTTCGTGTTC CCGTGGCTGC GACGATCGGG AGACCGTGCC 
TTGTCCCGGG CCGCCATGGA AGTGGCTCTT CGAGGCGTGC GGAAAGTCCT CTGTGTGGCG 
GAGAAAAATG ACGCGGCCAA GGGGATCGCC GACCTGCTGT CCAACGGTCG CATGAGGCGG 
AGGGAAGGAC TTTCTAAGTT CAACAAGATC TACGAATTTG ATTATCATCT ATTTGGCCAG 
AATGTTACCA TGGTAATGAC TTCAGTCTCT GGACATTTGC TGGCTCATGA TTTCCAGATG 
AAGTTTCGAA AGTGGCAGAG CTGCAACCCC CTTGTCCTCT TTGAAGCAGA AATCGAAAAA 
TACTGCCCAG ACAATTTTGT AGATATCAAG AAAACTCTGG AACGAGAGAC ACAGCAATGC 
CAGGCCCTGG TGATCTGGAC TGACTGTGAC AGAGAAGGCG AAAACATCGG GTTTGAAATT 
ATCCACGTGT GCAAAGCTGT GAAGCCTGGT CTCCAGGTGT TGAGAGCCCG TTTCTCTGAG 
ATCACACCCC GTGCTGTCAG GATGGCCTGT GAAAACCTAG CTGAACCTGA TCAGAAAGTG 
AGTGATGCTG TGGATGTGAG GCAGGAGCTG GACCTAAGGA TTGGAGCTGC ATTTACTAGG 
TTCCAGACCC TGCGTCTTCA AAAGATTTTT CCCGAGGTGC TGGCAGAGCA GCTCATCAGC 
TATGGCAGCT GCCAGTTCCC AACACTGGGG TTTGTGGTAG AGAGGTTCAA AGCCATTCAG 
GCTTTTGTAC CAGAAATCTT CCACAGAATT AAAGTAACTC ATGACCACAA AGATGGTACT 
GTAGAGTTCA ACTGGAAAAG GCATCGTCTC TTTAATCACA CAGCTTGTCT TGTCCTCTAT 
CAGTTGTGTA TGGAGGATCC CATGGCAACT GTGGTAGAGG TCAAGTCCAA GCCAAAGAGC 
AAGTGGAGGC CACAAGCTTT GGACACCGTG GAGCTGGAGA AACTAGCTTC TCGAAAATTG 
AGAATAAATG CTAAAGAAAC CATGAGAATT GCTGAGAGGC TCTACACCCA AGGGTACATC 
AGCTATCCCC GAACAGAAAC AAACATTTTC CCCAAAGACT TAAACCTGAT GGTGTTGGTA 
GAACAGCAGA CTCCAGATCC ACACTGGGGG GCCTTTGCCC AGAGCATTCT AGAGAGGGGT 
GGCCCCACCC CACGCAATGG AAGCAAGTCT GACCAAGCTC ACCCTCCCAT TCACCCCACT 
AAATACACCA GTAACTTGCA GGGAGATGAA CGGCGACTGT ATGAGTTCAT CGTTCGCCAT 
TTTCTGGCTT GCTGCTCCCA GGATGCTCAG GGGCAGGAAA CCACCGTGGA AATTGACATT 
GCTCAGGAAC GCTTTGTGGC TCATGGCCTT GTGATTCTGG CCCGAAACTA TCTGGATGTG 
TATCCATATG ATCATTGGAG TGACAAGCTC CTCCCTGTCT ATGAGCAAGG CTCCTGCTTT 
CAGCCCAGCA CCGTAGAGAT GGTGGATGGT GAGACCAGCC CACCCCAGCT GCTCACTGAG 
GCTGACCTCA TCGCCCTCAT GGAGAAGCAT GGCATCGGTA CTGATGCCAC TCACGCAGAA 
CATATTGAAA CCATCAAGGC ACGGATGTAT GTGGGGCTCA CCTCAGACAA GCGGTTCCTC 
CCCGGGCACC TGGGCATGGG ACTTGTGGAA GGTTACGATT CCATGGGCTA TGAGATGTCC 
AAACCTGACC TCCGGGCTGA GCTGGAAGCT GATCTGAAAC TGATCTGTGA GGGCAAGAAA 
GACAAGCTTG TGGTTCTAAG ACAGCAAGTG CAGAAATATA AGCAGGTTTT CATTGAAGCA 
GTGGCTAAGG CGAAGAAATT GGATGAGGCC TTGTCCCAGT ATTTTGGGGA AGGGGCAGAG 
ATGGCCCAAC AAGAAGAGAT CTACCCACCC ATGCCAGACC CCATCCGGAA GTGCCCACAA 
TGCAACAAGG ACATGGTCCT CAAGACCAAG AAGAGTGGCG GGTTCTACCT CAGTTGCACA 
GGTTTCCCAG AATGTCGATC AGCTGTATGG CTTCCTGACT CGGTGTTGGA GGCCAGCAAG 
GATGGGAGTG TGTGTTCAGT TTGTCAGCCA CCCCCTGTAC ACAGGTTGAA ATTGAAGTTT 
AAGCGTGGCA GCCTTCCCCC AACCATGCCC CTGGAGTTTG TTGCTTGCAT CGGTGGATGT 
GATGAAACCC TGAAGGAGAT CTTGGGTCTG AGATTTTCAC AGGGCCCCCC GAGAGCTATT 
CAGCCTTCTG GCCACCTGCA GGCTAGCCAA GCCCTGAACA GGATGGACAG TGGGCAGCAC 
AGCCACCCCC AGCCTCCTAG AAGCAGACAG ACTGGACCCT CAAAGCCTGT GGCCCAGACT 
CTCCTACCAC CCACTGCCAA TAGCGAAAGT AACTCTGTGA CCTGCAACTG TGGCCAGGAG 
GCCGTGCTGC TCACTGTCCG CAAGGAGGGC CCCAACCAGG GCCGGCAGTT CTACAAGTGC 
AACAGTGGCA GCTGCAACTT TTTCCTGTGG GCTGATGGCA ACCATCCTAC AACAGGAAGA 
CCCCCCACCT CTGCACCAAG GCCTCCCAGC AGCTCACTGG GACTCCCTCC AAGTTCATGC 
AGCTATCTGG GTGGTTTCGA CAACCCTGGC AATGGTGGTG GTGGTGGTGG TGGCACATCC 
TGCCTGTGTA GCCAGCCTGC TGTCACACGG ACTGTGCAGA AGGACGGGCC CAACAAAGGT 
CGCCAATTCC ACACATGCTC TAAGCCACGA GAGCAACAGT GTGGCTTCTT TCAGTGGGTT 
GATGAGAATA TAGCCCCAGG GACTTTTGTG GCCCTACCCT GGCCAGGAGG CAGAGGAAAA 
ACCCAAGGGC CAGAGGCCAG AAGCAAAAGA CCCAGGGCCA GTTCCTCAGA GGCAGGGTCC 
ACAGCAAAGA AGTCTCGGAA ATGCAGCCTT TGCCACCAGC CTGGACATAC TCGGCCCTTC 
TGTCCTCAGA ACAGATGA