RALGAPB cDNA ORF clone, Canis lupus familiaris(dog)

The following RALGAPB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RALGAPB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe24888 XM_005635049.3
Latest version!
Canis lupus familiaris Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe24888 XM_005635050.3
Latest version!
Canis lupus familiaris Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe24888 XM_005635048.3
Latest version!
Canis lupus familiaris Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe24888 XM_022409441.1
Latest version!
Canis lupus familiaris Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe24889 XM_005635051.2
Latest version!
Canis lupus familiaris Ral GTPase activating protein, beta subunit (non-catalytic) (RALGAPB), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe24889 XM_022409442.1
Latest version!
Canis lupus familiaris Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe24890 XM_022409443.1
Latest version!
Canis lupus familiaris Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe24890 XM_005635052.2
Latest version!
Canis lupus familiaris Ral GTPase activating protein, beta subunit (non-catalytic) (RALGAPB), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe24891 XM_022409444.1
Latest version!
Canis lupus familiaris Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe24891 XM_005635053.2
Latest version!
Canis lupus familiaris Ral GTPase activating protein, beta subunit (non-catalytic) (RALGAPB), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe24892 XM_022409445.1
Latest version!
Canis lupus familiaris Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe24892 XM_005635054.2
Latest version!
Canis lupus familiaris Ral GTPase activating protein, beta subunit (non-catalytic) (RALGAPB), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe24893 XM_847511.4
Latest version!
Canis lupus familiaris Ral GTPase activating protein, beta subunit (non-catalytic) (RALGAPB), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe24893 XM_022409446.1
Latest version!
Canis lupus familiaris Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe24894 XM_005635055.2
Latest version!
Canis lupus familiaris Ral GTPase activating protein, beta subunit (non-catalytic) (RALGAPB), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe24894 XM_022409447.1
Latest version!
Canis lupus familiaris Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OCe24888 XM_005635050.2 Canis lupus familiaris Ral GTPase activating protein, beta subunit (non-catalytic) (RALGAPB), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe24888 XM_005635049.2 Canis lupus familiaris Ral GTPase activating protein, beta subunit (non-catalytic) (RALGAPB), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe24888 XM_005635048.2 Canis lupus familiaris Ral GTPase activating protein, beta subunit (non-catalytic) (RALGAPB), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe24888
    Clone ID Related Accession (Same CDS sequence) XM_022409441.1 , XM_005635050.3 , XM_005635049.2 , XM_005635048.2 , XM_005635050.2 , XM_005635049.3 , XM_005635048.3
    Accession Version XM_022409441.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4533bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ral GTPase-activating protein subunit beta isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATCCAGA ATGATCAGGG CCACACGAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGG CGAGAGGTGG CAAATGCAGT GGTCCATCCC
    CTTGGGCAGG CCTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAGTTTGTT AAAAACTGAC
    AAAGAAGTGA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGTGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTATTGCCG
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC TATATGTTCA AAGTATACTA
    AAACACCTGC AAAATCTTTT TGTACCAAGA CAGGAACAGG GCTCCAGTCA GATCCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTAGCCCGTG AGTCATCCAT CATGGCACGA
    GAAACGTGGG AAGTCTTACT ATTGTTTCTT CTGCAGATTA ATGACATACT TCTAGCCCCG
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTTTGGT TACTAGCTTG TACTCGGTGC TTTCCAACAC CTCCTTATTG GAAAACCGCC
    AAGGAGATGG TGGCAAACTG GAGGCACCAC CCGGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ACCTGTGCCC TCACTTCAAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTCCATTT
    AAAGTTCCTG ATGAAGATGC CAGTCTGATC CCTCCTGAAA TGGATAATGA GTGTGTAGCA
    CAGACATGGT TTCGCTTCTT ACATATGTTA AGTAATCCCG TGGATTTGAG TAACCCGGCC
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTAA ATGTGAGCGG AATGCCACAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTAGTGGA TGCATTCTTA GGCATTTCTA GACCCCGATC AGACAGTGCT
    CCTCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGCA CTGCTGTCAG TACCACCCCA
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC GAAAGTTCAG CACCAGACAT CCTCCACCTC TCCTCTGTCA
    AGTCCCAATC AGACTAGTTC TGAACCCCGG CCACTGCCTG CCCCTCGAAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCAT CTTTTATCCA AATTCTTCTT
    TCTTATAAAT CTTCCATTAC AACACAAGCT AGCATGGAGT TTCGACGGAA AGGGTCACAA
    ATGTCCACAG ACACCATGGT TTCCAATCCT ATGTTTGATG CAAGTGAATT TCCTGATAAC
    TATGAAGCAG GAAGAGCTGA GGCTTGTGGG ACACTGTGTA GGATTTTTTG TAGCAAGAAG
    ACTGGAGAAG AGATTCTGCC AGCTTATTTA TCCAGATTTT ACATGCTTTT AATTCAAGGT
    TTGCAGATAA ATGATTATGT GTGCCATCCT GTCTTGGCCA GCGTTATTCT AAACTCCCCT
    CCTTTGTTCT GCTGTGACTT GAAAGGGATT GATGTTGTGG TTCCTTACTT TATTTCAGCT
    CTTGAAACCA TTTTGCCTGA CAGAGAACTC TCAAAATTCA AAAGCTATGT AAATCCAACA
    GAATTGAGAA GATCTTCCAT TAATATCCTC CTTTCTTTAT TGCCCCTCCC TCATCATTTT
    GGCACAGTCA GATCTGAGGT GGTCCTGGAA GGGAAGTTTA GTAATGATGA CAGCTCTTCA
    TATGATAAAC CAATAACTTT TTTGTCCCTG AAGTTGAGAC TTGTGAATAT ACTAATAGGT
    GCCTTGCAAA CGGAAACGGA CCCCAACAAC ACTCAAATGA TACTAGGGGC AATGTTAAAT
    ATTGTTCAAG ATTCAGCACT TTTAGAAGCC ATTGGTTGCC AAATGGAGAT GGGTGGTGGA
    GAAAATAACC TGAAGAGTCA TAGTCGTACT AATAGTGGTA TTAGTTCAGC AAGTGGTGGA
    AGCACAGAAC CCACAACTCC TGATAGTGAA AGACCTGCTC AAGCTCTCCT AAGGGATTAT
    GGATCGACAG CTCTTAATAC AGATTCAGCT GCTGGGCTCC TGATTCGCAG CATTCATCTT
    GTCACCCAAA GACTCAACTC TCAGTGGCGG CAAGACATGA GCATATCACT GGCAGCGCTG
    GAGCTCCTCT CTGGCCTGGC AAAGGTGAAG GTGATGGTTG ACTCGGGAGA CCGGAAACGA
    GCCATCAGTT CTGTGTGCAG CTACATCGTG TACCAATGTA GTCGGCCAGC TCCTCTTCAC
    TCCCGCGATC TGCACTCCAT GATAGTAGCA GCTTTTCAGT GTCTCTGTGT CTGGCTGACA
    GAGCATCCTG ATATGCTTGA TGAAAAGGAC TGTCTTAAGG AAGTATTGGA GATTGTGGAA
    CTGGGTATTT CAGGAAGTAA GTCCAAGAAC AGTGAGCAAG AGGTCAAGTA CAAAGGAGAT
    AAGGAGCCAA ACCCTGCATC TATGAGGGTA AAGGATGCTG CTGAAGCCAC TCTAACATGT
    ATTATGCAGT TGCTCGGCGC GTTTCCTTCA CCCAGTGGTC CTGCCTCTCC TTGTAGTCTG
    GTGAATGAGA CCACTTTGAT TAAATACTCA AGGCTGCCAA CCATAAACAA GCATAGTTTC
    CGGTACTTTG TCTTGGATAA CAGTGTCATC CTGGCAATGC TGGAGCAACC TCTTGGAAAT
    GAACAGAATG ATTTTTTTCC CTCTGTCACT GTGCTGGTTC GAGGAATGTC TGGAAGACTT
    GCTTGGGCAC AACAACTTTG CCTTTTACCC AGAGGAGCAA AAGCAAATCA GAAGCTTTTT
    GTACCTGAAC CTCGTCCAAT TCCTAAAAAT GATGTTGGAT TTAAATATAC TGTCAAACAT
    CGACCATTTC CTGAAGAGGT GGATAAGATT CCTTTTGTGA AAGCAGATCT GAGCATTCCA
    GATTTGCATG AAATTGTCAC TGAAGAATTA GAAGAGAGAC ATGAAAAATT AAGGAATGGC
    ATGGCCCAGC AGATTGCTTA TGAAATACAC CTTGAACAAC AAAGTGAGGA AGAATTGCAG
    AAGAGAAGTT TTCCTGATCC AATTACGGAT TGTAAGCCTC CACCTCCTGC CCAAGAATTC
    CAAACAGCAC GCCTCTTCCT TTCACACTTT GGATTTTTGT CTTTAGAAGC ATTGAAGGAA
    CCTGCAAATA GTCGCCTACC TCCTCACCTT ATTGCACTTG ACTCCACGAT ACCTGGATTT
    TTTGATGACA TTGGATATCT GGATCTCTTG CCGTGTCGTC CCTTTGACAC AGTTTTTATT
    TTCTATATGA AACCAGGTCA GAAAACAAAC CAGGAGATTT TAAAGAATGT GGAATCTTCC
    AGAAATGTTC AGCCACATTT CCTAGAATTT TTGCTCTCCC TTGGCTGGTC AGTAGATGTG
    GGCAGACACC CAGGTTGGAC TGGGCATGTT TCTACCAGTT GGTCTATTAA CAGTTGTGAT
    GATGGTGAAG GACCTGAACA AGATGAAGTG ATTTCCTCTG AAGACATTGG GGCTAGCATT
    TTCAATGGAC AGAAGAAGGT GCTGTATTAT GCTGATGCCC TTACGGAAAT AGCTTTTGTA
    GTTCCTTCTC CTGTGGAGTC TTTAACTGAT TCATTAGAAA GTAACATCTC GGACCAAGAT
    AGTGATTCAA ATATGGATCT TATGCCTGGA ATTCTGAAAC AGCCATCCCT GACACTTGAG
    CTTTTCCCTA ATCACACAGA CAATCTTAAT TCCTCACAGC GGCTTAGTCC CAGTTCCAGA
    ATGAAGAAGC TGCCTCAGGG TCGCCCTGTG CCTCCCCTTG GACCTGAGAC AAGAGTTTCT
    GTAGTCTGGG TGGAACGCTA TGATGATATA GAGAACTTTC CCCTCTCAGA CCTGCTGACA
    GAGATCAGTA CTGGTGTGGA AACTACTGCA AACAGCAGCA CTTCTCTGAG ATCTACCACT
    CTTGAAAAAG AAGTTCCTGT CATCTTTATC CACCCTTTAA ACACTGGATT ATTCCGGATA
    AAAATTCAAG GAGCCACTGG AAAATTTAAC ATGGTCATCC CGCTCGTGGA TGGGATGATT
    GTCAGCAGAC GAGCACTTGG TTTTCTGGTG AGGCAGACTG TAATTAATAT TTGTAGAAGA
    AAGAGACTGG AGAGTGACTC CTACAGTCCA CCACATGTCC GCCGGAAACA GAAAATCACC
    GACATTGTCA ACAAGTACCG GAACAAGCAG TTGGAGCCAG AGTTTTATAC TTCACTTTTC
    CAGGAGGTTG GACTCAAGAA CTGCTCTTCT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022265149.1
    CDS370..4902
    Translation

    Target ORF information:

    RefSeq Version XM_022409441.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022409441.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATCCAGA ATGATCAGGG CCACACGAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGG CGAGAGGTGG CAAATGCAGT GGTCCATCCC
    CTTGGGCAGG CCTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAGTTTGTT AAAAACTGAC
    AAAGAAGTGA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGTGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTATTGCCG
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC TATATGTTCA AAGTATACTA
    AAACACCTGC AAAATCTTTT TGTACCAAGA CAGGAACAGG GCTCCAGTCA GATCCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTAGCCCGTG AGTCATCCAT CATGGCACGA
    GAAACGTGGG AAGTCTTACT ATTGTTTCTT CTGCAGATTA ATGACATACT TCTAGCCCCG
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTTTGGT TACTAGCTTG TACTCGGTGC TTTCCAACAC CTCCTTATTG GAAAACCGCC
    AAGGAGATGG TGGCAAACTG GAGGCACCAC CCGGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ACCTGTGCCC TCACTTCAAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTCCATTT
    AAAGTTCCTG ATGAAGATGC CAGTCTGATC CCTCCTGAAA TGGATAATGA GTGTGTAGCA
    CAGACATGGT TTCGCTTCTT ACATATGTTA AGTAATCCCG TGGATTTGAG TAACCCGGCC
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTAA ATGTGAGCGG AATGCCACAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTAGTGGA TGCATTCTTA GGCATTTCTA GACCCCGATC AGACAGTGCT
    CCTCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGCA CTGCTGTCAG TACCACCCCA
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC GAAAGTTCAG CACCAGACAT CCTCCACCTC TCCTCTGTCA
    AGTCCCAATC AGACTAGTTC TGAACCCCGG CCACTGCCTG CCCCTCGAAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCAT CTTTTATCCA AATTCTTCTT
    TCTTATAAAT CTTCCATTAC AACACAAGCT AGCATGGAGT TTCGACGGAA AGGGTCACAA
    ATGTCCACAG ACACCATGGT TTCCAATCCT ATGTTTGATG CAAGTGAATT TCCTGATAAC
    TATGAAGCAG GAAGAGCTGA GGCTTGTGGG ACACTGTGTA GGATTTTTTG TAGCAAGAAG
    ACTGGAGAAG AGATTCTGCC AGCTTATTTA TCCAGATTTT ACATGCTTTT AATTCAAGGT
    TTGCAGATAA ATGATTATGT GTGCCATCCT GTCTTGGCCA GCGTTATTCT AAACTCCCCT
    CCTTTGTTCT GCTGTGACTT GAAAGGGATT GATGTTGTGG TTCCTTACTT TATTTCAGCT
    CTTGAAACCA TTTTGCCTGA CAGAGAACTC TCAAAATTCA AAAGCTATGT AAATCCAACA
    GAATTGAGAA GATCTTCCAT TAATATCCTC CTTTCTTTAT TGCCCCTCCC TCATCATTTT
    GGCACAGTCA GATCTGAGGT GGTCCTGGAA GGGAAGTTTA GTAATGATGA CAGCTCTTCA
    TATGATAAAC CAATAACTTT TTTGTCCCTG AAGTTGAGAC TTGTGAATAT ACTAATAGGT
    GCCTTGCAAA CGGAAACGGA CCCCAACAAC ACTCAAATGA TACTAGGGGC AATGTTAAAT
    ATTGTTCAAG ATTCAGCACT TTTAGAAGCC ATTGGTTGCC AAATGGAGAT GGGTGGTGGA
    GAAAATAACC TGAAGAGTCA TAGTCGTACT AATAGTGGTA TTAGTTCAGC AAGTGGTGGA
    AGCACAGAAC CCACAACTCC TGATAGTGAA AGACCTGCTC AAGCTCTCCT AAGGGATTAT
    GGATCGACAG CTCTTAATAC AGATTCAGCT GCTGGGCTCC TGATTCGCAG CATTCATCTT
    GTCACCCAAA GACTCAACTC TCAGTGGCGG CAAGACATGA GCATATCACT GGCAGCGCTG
    GAGCTCCTCT CTGGCCTGGC AAAGGTGAAG GTGATGGTTG ACTCGGGAGA CCGGAAACGA
    GCCATCAGTT CTGTGTGCAG CTACATCGTG TACCAATGTA GTCGGCCAGC TCCTCTTCAC
    TCCCGCGATC TGCACTCCAT GATAGTAGCA GCTTTTCAGT GTCTCTGTGT CTGGCTGACA
    GAGCATCCTG ATATGCTTGA TGAAAAGGAC TGTCTTAAGG AAGTATTGGA GATTGTGGAA
    CTGGGTATTT CAGGAAGTAA GTCCAAGAAC AGTGAGCAAG AGGTCAAGTA CAAAGGAGAT
    AAGGAGCCAA ACCCTGCATC TATGAGGGTA AAGGATGCTG CTGAAGCCAC TCTAACATGT
    ATTATGCAGT TGCTCGGCGC GTTTCCTTCA CCCAGTGGTC CTGCCTCTCC TTGTAGTCTG
    GTGAATGAGA CCACTTTGAT TAAATACTCA AGGCTGCCAA CCATAAACAA GCATAGTTTC
    CGGTACTTTG TCTTGGATAA CAGTGTCATC CTGGCAATGC TGGAGCAACC TCTTGGAAAT
    GAACAGAATG ATTTTTTTCC CTCTGTCACT GTGCTGGTTC GAGGAATGTC TGGAAGACTT
    GCTTGGGCAC AACAACTTTG CCTTTTACCC AGAGGAGCAA AAGCAAATCA GAAGCTTTTT
    GTACCTGAAC CTCGTCCAAT TCCTAAAAAT GATGTTGGAT TTAAATATAC TGTCAAACAT
    CGACCATTTC CTGAAGAGGT GGATAAGATT CCTTTTGTGA AAGCAGATCT GAGCATTCCA
    GATTTGCATG AAATTGTCAC TGAAGAATTA GAAGAGAGAC ATGAAAAATT AAGGAATGGC
    ATGGCCCAGC AGATTGCTTA TGAAATACAC CTTGAACAAC AAAGTGAGGA AGAATTGCAG
    AAGAGAAGTT TTCCTGATCC AATTACGGAT TGTAAGCCTC CACCTCCTGC CCAAGAATTC
    CAAACAGCAC GCCTCTTCCT TTCACACTTT GGATTTTTGT CTTTAGAAGC ATTGAAGGAA
    CCTGCAAATA GTCGCCTACC TCCTCACCTT ATTGCACTTG ACTCCACGAT ACCTGGATTT
    TTTGATGACA TTGGATATCT GGATCTCTTG CCGTGTCGTC CCTTTGACAC AGTTTTTATT
    TTCTATATGA AACCAGGTCA GAAAACAAAC CAGGAGATTT TAAAGAATGT GGAATCTTCC
    AGAAATGTTC AGCCACATTT CCTAGAATTT TTGCTCTCCC TTGGCTGGTC AGTAGATGTG
    GGCAGACACC CAGGTTGGAC TGGGCATGTT TCTACCAGTT GGTCTATTAA CAGTTGTGAT
    GATGGTGAAG GACCTGAACA AGATGAAGTG ATTTCCTCTG AAGACATTGG GGCTAGCATT
    TTCAATGGAC AGAAGAAGGT GCTGTATTAT GCTGATGCCC TTACGGAAAT AGCTTTTGTA
    GTTCCTTCTC CTGTGGAGTC TTTAACTGAT TCATTAGAAA GTAACATCTC GGACCAAGAT
    AGTGATTCAA ATATGGATCT TATGCCTGGA ATTCTGAAAC AGCCATCCCT GACACTTGAG
    CTTTTCCCTA ATCACACAGA CAATCTTAAT TCCTCACAGC GGCTTAGTCC CAGTTCCAGA
    ATGAAGAAGC TGCCTCAGGG TCGCCCTGTG CCTCCCCTTG GACCTGAGAC AAGAGTTTCT
    GTAGTCTGGG TGGAACGCTA TGATGATATA GAGAACTTTC CCCTCTCAGA CCTGCTGACA
    GAGATCAGTA CTGGTGTGGA AACTACTGCA AACAGCAGCA CTTCTCTGAG ATCTACCACT
    CTTGAAAAAG AAGTTCCTGT CATCTTTATC CACCCTTTAA ACACTGGATT ATTCCGGATA
    AAAATTCAAG GAGCCACTGG AAAATTTAAC ATGGTCATCC CGCTCGTGGA TGGGATGATT
    GTCAGCAGAC GAGCACTTGG TTTTCTGGTG AGGCAGACTG TAATTAATAT TTGTAGAAGA
    AAGAGACTGG AGAGTGACTC CTACAGTCCA CCACATGTCC GCCGGAAACA GAAAATCACC
    GACATTGTCA ACAAGTACCG GAACAAGCAG TTGGAGCCAG AGTTTTATAC TTCACTTTTC
    CAGGAGGTTG GACTCAAGAA CTGCTCTTCT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe24888
    Clone ID Related Accession (Same CDS sequence) XM_022409441.1 , XM_005635050.3 , XM_005635049.2 , XM_005635048.2 , XM_005635050.2 , XM_005635049.3 , XM_005635048.3
    Accession Version XM_005635050.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4533bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ral GTPase-activating protein subunit beta isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005635050.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATCCAGA ATGATCAGGG CCACACGAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGG CGAGAGGTGG CAAATGCAGT GGTCCATCCC
    CTTGGGCAGG CCTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAGTTTGTT AAAAACTGAC
    AAAGAAGTGA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGTGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTATTGCCG
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC TATATGTTCA AAGTATACTA
    AAACACCTGC AAAATCTTTT TGTACCAAGA CAGGAACAGG GCTCCAGTCA GATCCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTAGCCCGTG AGTCATCCAT CATGGCACGA
    GAAACGTGGG AAGTCTTACT ATTGTTTCTT CTGCAGATTA ATGACATACT TCTAGCCCCG
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTTTGGT TACTAGCTTG TACTCGGTGC TTTCCAACAC CTCCTTATTG GAAAACCGCC
    AAGGAGATGG TGGCAAACTG GAGGCACCAC CCGGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ACCTGTGCCC TCACTTCAAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTCCATTT
    AAAGTTCCTG ATGAAGATGC CAGTCTGATC CCTCCTGAAA TGGATAATGA GTGTGTAGCA
    CAGACATGGT TTCGCTTCTT ACATATGTTA AGTAATCCCG TGGATTTGAG TAACCCGGCC
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTAA ATGTGAGCGG AATGCCACAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTAGTGGA TGCATTCTTA GGCATTTCTA GACCCCGATC AGACAGTGCT
    CCTCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGCA CTGCTGTCAG TACCACCCCA
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC GAAAGTTCAG CACCAGACAT CCTCCACCTC TCCTCTGTCA
    AGTCCCAATC AGACTAGTTC TGAACCCCGG CCACTGCCTG CCCCTCGAAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCAT CTTTTATCCA AATTCTTCTT
    TCTTATAAAT CTTCCATTAC AACACAAGCT AGCATGGAGT TTCGACGGAA AGGGTCACAA
    ATGTCCACAG ACACCATGGT TTCCAATCCT ATGTTTGATG CAAGTGAATT TCCTGATAAC
    TATGAAGCAG GAAGAGCTGA GGCTTGTGGG ACACTGTGTA GGATTTTTTG TAGCAAGAAG
    ACTGGAGAAG AGATTCTGCC AGCTTATTTA TCCAGATTTT ACATGCTTTT AATTCAAGGT
    TTGCAGATAA ATGATTATGT GTGCCATCCT GTCTTGGCCA GCGTTATTCT AAACTCCCCT
    CCTTTGTTCT GCTGTGACTT GAAAGGGATT GATGTTGTGG TTCCTTACTT TATTTCAGCT
    CTTGAAACCA TTTTGCCTGA CAGAGAACTC TCAAAATTCA AAAGCTATGT AAATCCAACA
    GAATTGAGAA GATCTTCCAT TAATATCCTC CTTTCTTTAT TGCCCCTCCC TCATCATTTT
    GGCACAGTCA GATCTGAGGT GGTCCTGGAA GGGAAGTTTA GTAATGATGA CAGCTCTTCA
    TATGATAAAC CAATAACTTT TTTGTCCCTG AAGTTGAGAC TTGTGAATAT ACTAATAGGT
    GCCTTGCAAA CGGAAACGGA CCCCAACAAC ACTCAAATGA TACTAGGGGC AATGTTAAAT
    ATTGTTCAAG ATTCAGCACT TTTAGAAGCC ATTGGTTGCC AAATGGAGAT GGGTGGTGGA
    GAAAATAACC TGAAGAGTCA TAGTCGTACT AATAGTGGTA TTAGTTCAGC AAGTGGTGGA
    AGCACAGAAC CCACAACTCC TGATAGTGAA AGACCTGCTC AAGCTCTCCT AAGGGATTAT
    GGATCGACAG CTCTTAATAC AGATTCAGCT GCTGGGCTCC TGATTCGCAG CATTCATCTT
    GTCACCCAAA GACTCAACTC TCAGTGGCGG CAAGACATGA GCATATCACT GGCAGCGCTG
    GAGCTCCTCT CTGGCCTGGC AAAGGTGAAG GTGATGGTTG ACTCGGGAGA CCGGAAACGA
    GCCATCAGTT CTGTGTGCAG CTACATCGTG TACCAATGTA GTCGGCCAGC TCCTCTTCAC
    TCCCGCGATC TGCACTCCAT GATAGTAGCA GCTTTTCAGT GTCTCTGTGT CTGGCTGACA
    GAGCATCCTG ATATGCTTGA TGAAAAGGAC TGTCTTAAGG AAGTATTGGA GATTGTGGAA
    CTGGGTATTT CAGGAAGTAA GTCCAAGAAC AGTGAGCAAG AGGTCAAGTA CAAAGGAGAT
    AAGGAGCCAA ACCCTGCATC TATGAGGGTA AAGGATGCTG CTGAAGCCAC TCTAACATGT
    ATTATGCAGT TGCTCGGCGC GTTTCCTTCA CCCAGTGGTC CTGCCTCTCC TTGTAGTCTG
    GTGAATGAGA CCACTTTGAT TAAATACTCA AGGCTGCCAA CCATAAACAA GCATAGTTTC
    CGGTACTTTG TCTTGGATAA CAGTGTCATC CTGGCAATGC TGGAGCAACC TCTTGGAAAT
    GAACAGAATG ATTTTTTTCC CTCTGTCACT GTGCTGGTTC GAGGAATGTC TGGAAGACTT
    GCTTGGGCAC AACAACTTTG CCTTTTACCC AGAGGAGCAA AAGCAAATCA GAAGCTTTTT
    GTACCTGAAC CTCGTCCAAT TCCTAAAAAT GATGTTGGAT TTAAATATAC TGTCAAACAT
    CGACCATTTC CTGAAGAGGT GGATAAGATT CCTTTTGTGA AAGCAGATCT GAGCATTCCA
    GATTTGCATG AAATTGTCAC TGAAGAATTA GAAGAGAGAC ATGAAAAATT AAGGAATGGC
    ATGGCCCAGC AGATTGCTTA TGAAATACAC CTTGAACAAC AAAGTGAGGA AGAATTGCAG
    AAGAGAAGTT TTCCTGATCC AATTACGGAT TGTAAGCCTC CACCTCCTGC CCAAGAATTC
    CAAACAGCAC GCCTCTTCCT TTCACACTTT GGATTTTTGT CTTTAGAAGC ATTGAAGGAA
    CCTGCAAATA GTCGCCTACC TCCTCACCTT ATTGCACTTG ACTCCACGAT ACCTGGATTT
    TTTGATGACA TTGGATATCT GGATCTCTTG CCGTGTCGTC CCTTTGACAC AGTTTTTATT
    TTCTATATGA AACCAGGTCA GAAAACAAAC CAGGAGATTT TAAAGAATGT GGAATCTTCC
    AGAAATGTTC AGCCACATTT CCTAGAATTT TTGCTCTCCC TTGGCTGGTC AGTAGATGTG
    GGCAGACACC CAGGTTGGAC TGGGCATGTT TCTACCAGTT GGTCTATTAA CAGTTGTGAT
    GATGGTGAAG GACCTGAACA AGATGAAGTG ATTTCCTCTG AAGACATTGG GGCTAGCATT
    TTCAATGGAC AGAAGAAGGT GCTGTATTAT GCTGATGCCC TTACGGAAAT AGCTTTTGTA
    GTTCCTTCTC CTGTGGAGTC TTTAACTGAT TCATTAGAAA GTAACATCTC GGACCAAGAT
    AGTGATTCAA ATATGGATCT TATGCCTGGA ATTCTGAAAC AGCCATCCCT GACACTTGAG
    CTTTTCCCTA ATCACACAGA CAATCTTAAT TCCTCACAGC GGCTTAGTCC CAGTTCCAGA
    ATGAAGAAGC TGCCTCAGGG TCGCCCTGTG CCTCCCCTTG GACCTGAGAC AAGAGTTTCT
    GTAGTCTGGG TGGAACGCTA TGATGATATA GAGAACTTTC CCCTCTCAGA CCTGCTGACA
    GAGATCAGTA CTGGTGTGGA AACTACTGCA AACAGCAGCA CTTCTCTGAG ATCTACCACT
    CTTGAAAAAG AAGTTCCTGT CATCTTTATC CACCCTTTAA ACACTGGATT ATTCCGGATA
    AAAATTCAAG GAGCCACTGG AAAATTTAAC ATGGTCATCC CGCTCGTGGA TGGGATGATT
    GTCAGCAGAC GAGCACTTGG TTTTCTGGTG AGGCAGACTG TAATTAATAT TTGTAGAAGA
    AAGAGACTGG AGAGTGACTC CTACAGTCCA CCACATGTCC GCCGGAAACA GAAAATCACC
    GACATTGTCA ACAAGTACCG GAACAAGCAG TTGGAGCCAG AGTTTTATAC TTCACTTTTC
    CAGGAGGTTG GACTCAAGAA CTGCTCTTCT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005635107.1
    CDS265..4797
    Translation

    Target ORF information:

    RefSeq Version XM_005635050.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005635050.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATCCAGA ATGATCAGGG CCACACGAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGG CGAGAGGTGG CAAATGCAGT GGTCCATCCC
    CTTGGGCAGG CCTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAGTTTGTT AAAAACTGAC
    AAAGAAGTGA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGTGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTATTGCCG
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC TATATGTTCA AAGTATACTA
    AAACACCTGC AAAATCTTTT TGTACCAAGA CAGGAACAGG GCTCCAGTCA GATCCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTAGCCCGTG AGTCATCCAT CATGGCACGA
    GAAACGTGGG AAGTCTTACT ATTGTTTCTT CTGCAGATTA ATGACATACT TCTAGCCCCG
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTTTGGT TACTAGCTTG TACTCGGTGC TTTCCAACAC CTCCTTATTG GAAAACCGCC
    AAGGAGATGG TGGCAAACTG GAGGCACCAC CCGGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ACCTGTGCCC TCACTTCAAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTCCATTT
    AAAGTTCCTG ATGAAGATGC CAGTCTGATC CCTCCTGAAA TGGATAATGA GTGTGTAGCA
    CAGACATGGT TTCGCTTCTT ACATATGTTA AGTAATCCCG TGGATTTGAG TAACCCGGCC
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTAA ATGTGAGCGG AATGCCACAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTAGTGGA TGCATTCTTA GGCATTTCTA GACCCCGATC AGACAGTGCT
    CCTCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGCA CTGCTGTCAG TACCACCCCA
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC GAAAGTTCAG CACCAGACAT CCTCCACCTC TCCTCTGTCA
    AGTCCCAATC AGACTAGTTC TGAACCCCGG CCACTGCCTG CCCCTCGAAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCAT CTTTTATCCA AATTCTTCTT
    TCTTATAAAT CTTCCATTAC AACACAAGCT AGCATGGAGT TTCGACGGAA AGGGTCACAA
    ATGTCCACAG ACACCATGGT TTCCAATCCT ATGTTTGATG CAAGTGAATT TCCTGATAAC
    TATGAAGCAG GAAGAGCTGA GGCTTGTGGG ACACTGTGTA GGATTTTTTG TAGCAAGAAG
    ACTGGAGAAG AGATTCTGCC AGCTTATTTA TCCAGATTTT ACATGCTTTT AATTCAAGGT
    TTGCAGATAA ATGATTATGT GTGCCATCCT GTCTTGGCCA GCGTTATTCT AAACTCCCCT
    CCTTTGTTCT GCTGTGACTT GAAAGGGATT GATGTTGTGG TTCCTTACTT TATTTCAGCT
    CTTGAAACCA TTTTGCCTGA CAGAGAACTC TCAAAATTCA AAAGCTATGT AAATCCAACA
    GAATTGAGAA GATCTTCCAT TAATATCCTC CTTTCTTTAT TGCCCCTCCC TCATCATTTT
    GGCACAGTCA GATCTGAGGT GGTCCTGGAA GGGAAGTTTA GTAATGATGA CAGCTCTTCA
    TATGATAAAC CAATAACTTT TTTGTCCCTG AAGTTGAGAC TTGTGAATAT ACTAATAGGT
    GCCTTGCAAA CGGAAACGGA CCCCAACAAC ACTCAAATGA TACTAGGGGC AATGTTAAAT
    ATTGTTCAAG ATTCAGCACT TTTAGAAGCC ATTGGTTGCC AAATGGAGAT GGGTGGTGGA
    GAAAATAACC TGAAGAGTCA TAGTCGTACT AATAGTGGTA TTAGTTCAGC AAGTGGTGGA
    AGCACAGAAC CCACAACTCC TGATAGTGAA AGACCTGCTC AAGCTCTCCT AAGGGATTAT
    GGATCGACAG CTCTTAATAC AGATTCAGCT GCTGGGCTCC TGATTCGCAG CATTCATCTT
    GTCACCCAAA GACTCAACTC TCAGTGGCGG CAAGACATGA GCATATCACT GGCAGCGCTG
    GAGCTCCTCT CTGGCCTGGC AAAGGTGAAG GTGATGGTTG ACTCGGGAGA CCGGAAACGA
    GCCATCAGTT CTGTGTGCAG CTACATCGTG TACCAATGTA GTCGGCCAGC TCCTCTTCAC
    TCCCGCGATC TGCACTCCAT GATAGTAGCA GCTTTTCAGT GTCTCTGTGT CTGGCTGACA
    GAGCATCCTG ATATGCTTGA TGAAAAGGAC TGTCTTAAGG AAGTATTGGA GATTGTGGAA
    CTGGGTATTT CAGGAAGTAA GTCCAAGAAC AGTGAGCAAG AGGTCAAGTA CAAAGGAGAT
    AAGGAGCCAA ACCCTGCATC TATGAGGGTA AAGGATGCTG CTGAAGCCAC TCTAACATGT
    ATTATGCAGT TGCTCGGCGC GTTTCCTTCA CCCAGTGGTC CTGCCTCTCC TTGTAGTCTG
    GTGAATGAGA CCACTTTGAT TAAATACTCA AGGCTGCCAA CCATAAACAA GCATAGTTTC
    CGGTACTTTG TCTTGGATAA CAGTGTCATC CTGGCAATGC TGGAGCAACC TCTTGGAAAT
    GAACAGAATG ATTTTTTTCC CTCTGTCACT GTGCTGGTTC GAGGAATGTC TGGAAGACTT
    GCTTGGGCAC AACAACTTTG CCTTTTACCC AGAGGAGCAA AAGCAAATCA GAAGCTTTTT
    GTACCTGAAC CTCGTCCAAT TCCTAAAAAT GATGTTGGAT TTAAATATAC TGTCAAACAT
    CGACCATTTC CTGAAGAGGT GGATAAGATT CCTTTTGTGA AAGCAGATCT GAGCATTCCA
    GATTTGCATG AAATTGTCAC TGAAGAATTA GAAGAGAGAC ATGAAAAATT AAGGAATGGC
    ATGGCCCAGC AGATTGCTTA TGAAATACAC CTTGAACAAC AAAGTGAGGA AGAATTGCAG
    AAGAGAAGTT TTCCTGATCC AATTACGGAT TGTAAGCCTC CACCTCCTGC CCAAGAATTC
    CAAACAGCAC GCCTCTTCCT TTCACACTTT GGATTTTTGT CTTTAGAAGC ATTGAAGGAA
    CCTGCAAATA GTCGCCTACC TCCTCACCTT ATTGCACTTG ACTCCACGAT ACCTGGATTT
    TTTGATGACA TTGGATATCT GGATCTCTTG CCGTGTCGTC CCTTTGACAC AGTTTTTATT
    TTCTATATGA AACCAGGTCA GAAAACAAAC CAGGAGATTT TAAAGAATGT GGAATCTTCC
    AGAAATGTTC AGCCACATTT CCTAGAATTT TTGCTCTCCC TTGGCTGGTC AGTAGATGTG
    GGCAGACACC CAGGTTGGAC TGGGCATGTT TCTACCAGTT GGTCTATTAA CAGTTGTGAT
    GATGGTGAAG GACCTGAACA AGATGAAGTG ATTTCCTCTG AAGACATTGG GGCTAGCATT
    TTCAATGGAC AGAAGAAGGT GCTGTATTAT GCTGATGCCC TTACGGAAAT AGCTTTTGTA
    GTTCCTTCTC CTGTGGAGTC TTTAACTGAT TCATTAGAAA GTAACATCTC GGACCAAGAT
    AGTGATTCAA ATATGGATCT TATGCCTGGA ATTCTGAAAC AGCCATCCCT GACACTTGAG
    CTTTTCCCTA ATCACACAGA CAATCTTAAT TCCTCACAGC GGCTTAGTCC CAGTTCCAGA
    ATGAAGAAGC TGCCTCAGGG TCGCCCTGTG CCTCCCCTTG GACCTGAGAC AAGAGTTTCT
    GTAGTCTGGG TGGAACGCTA TGATGATATA GAGAACTTTC CCCTCTCAGA CCTGCTGACA
    GAGATCAGTA CTGGTGTGGA AACTACTGCA AACAGCAGCA CTTCTCTGAG ATCTACCACT
    CTTGAAAAAG AAGTTCCTGT CATCTTTATC CACCCTTTAA ACACTGGATT ATTCCGGATA
    AAAATTCAAG GAGCCACTGG AAAATTTAAC ATGGTCATCC CGCTCGTGGA TGGGATGATT
    GTCAGCAGAC GAGCACTTGG TTTTCTGGTG AGGCAGACTG TAATTAATAT TTGTAGAAGA
    AAGAGACTGG AGAGTGACTC CTACAGTCCA CCACATGTCC GCCGGAAACA GAAAATCACC
    GACATTGTCA ACAAGTACCG GAACAAGCAG TTGGAGCCAG AGTTTTATAC TTCACTTTTC
    CAGGAGGTTG GACTCAAGAA CTGCTCTTCT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe24888
    Clone ID Related Accession (Same CDS sequence) XM_022409441.1 , XM_005635050.3 , XM_005635049.2 , XM_005635048.2 , XM_005635050.2 , XM_005635049.3 , XM_005635048.3
    Accession Version XM_005635049.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4533bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ral GTPase-activating protein subunit beta isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726101.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005635049.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATCCAGA ATGATCAGGG CCACACGAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGG CGAGAGGTGG CAAATGCAGT GGTCCATCCC
    CTTGGGCAGG CCTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAGTTTGTT AAAAACTGAC
    AAAGAAGTGA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGTGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTATTGCCG
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC TATATGTTCA AAGTATACTA
    AAACACCTGC AAAATCTTTT TGTACCAAGA CAGGAACAGG GCTCCAGTCA GATCCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTAGCCCGTG AGTCATCCAT CATGGCACGA
    GAAACGTGGG AAGTCTTACT ATTGTTTCTT CTGCAGATTA ATGACATACT TCTAGCCCCG
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTTTGGT TACTAGCTTG TACTCGGTGC TTTCCAACAC CTCCTTATTG GAAAACCGCC
    AAGGAGATGG TGGCAAACTG GAGGCACCAC CCGGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ACCTGTGCCC TCACTTCAAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTCCATTT
    AAAGTTCCTG ATGAAGATGC CAGTCTGATC CCTCCTGAAA TGGATAATGA GTGTGTAGCA
    CAGACATGGT TTCGCTTCTT ACATATGTTA AGTAATCCCG TGGATTTGAG TAACCCGGCC
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTAA ATGTGAGCGG AATGCCACAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTAGTGGA TGCATTCTTA GGCATTTCTA GACCCCGATC AGACAGTGCT
    CCTCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGCA CTGCTGTCAG TACCACCCCA
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC GAAAGTTCAG CACCAGACAT CCTCCACCTC TCCTCTGTCA
    AGTCCCAATC AGACTAGTTC TGAACCCCGG CCACTGCCTG CCCCTCGAAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCAT CTTTTATCCA AATTCTTCTT
    TCTTATAAAT CTTCCATTAC AACACAAGCT AGCATGGAGT TTCGACGGAA AGGGTCACAA
    ATGTCCACAG ACACCATGGT TTCCAATCCT ATGTTTGATG CAAGTGAATT TCCTGATAAC
    TATGAAGCAG GAAGAGCTGA GGCTTGTGGG ACACTGTGTA GGATTTTTTG TAGCAAGAAG
    ACTGGAGAAG AGATTCTGCC AGCTTATTTA TCCAGATTTT ACATGCTTTT AATTCAAGGT
    TTGCAGATAA ATGATTATGT GTGCCATCCT GTCTTGGCCA GCGTTATTCT AAACTCCCCT
    CCTTTGTTCT GCTGTGACTT GAAAGGGATT GATGTTGTGG TTCCTTACTT TATTTCAGCT
    CTTGAAACCA TTTTGCCTGA CAGAGAACTC TCAAAATTCA AAAGCTATGT AAATCCAACA
    GAATTGAGAA GATCTTCCAT TAATATCCTC CTTTCTTTAT TGCCCCTCCC TCATCATTTT
    GGCACAGTCA GATCTGAGGT GGTCCTGGAA GGGAAGTTTA GTAATGATGA CAGCTCTTCA
    TATGATAAAC CAATAACTTT TTTGTCCCTG AAGTTGAGAC TTGTGAATAT ACTAATAGGT
    GCCTTGCAAA CGGAAACGGA CCCCAACAAC ACTCAAATGA TACTAGGGGC AATGTTAAAT
    ATTGTTCAAG ATTCAGCACT TTTAGAAGCC ATTGGTTGCC AAATGGAGAT GGGTGGTGGA
    GAAAATAACC TGAAGAGTCA TAGTCGTACT AATAGTGGTA TTAGTTCAGC AAGTGGTGGA
    AGCACAGAAC CCACAACTCC TGATAGTGAA AGACCTGCTC AAGCTCTCCT AAGGGATTAT
    GGATCGACAG CTCTTAATAC AGATTCAGCT GCTGGGCTCC TGATTCGCAG CATTCATCTT
    GTCACCCAAA GACTCAACTC TCAGTGGCGG CAAGACATGA GCATATCACT GGCAGCGCTG
    GAGCTCCTCT CTGGCCTGGC AAAGGTGAAG GTGATGGTTG ACTCGGGAGA CCGGAAACGA
    GCCATCAGTT CTGTGTGCAG CTACATCGTG TACCAATGTA GTCGGCCAGC TCCTCTTCAC
    TCCCGCGATC TGCACTCCAT GATAGTAGCA GCTTTTCAGT GTCTCTGTGT CTGGCTGACA
    GAGCATCCTG ATATGCTTGA TGAAAAGGAC TGTCTTAAGG AAGTATTGGA GATTGTGGAA
    CTGGGTATTT CAGGAAGTAA GTCCAAGAAC AGTGAGCAAG AGGTCAAGTA CAAAGGAGAT
    AAGGAGCCAA ACCCTGCATC TATGAGGGTA AAGGATGCTG CTGAAGCCAC TCTAACATGT
    ATTATGCAGT TGCTCGGCGC GTTTCCTTCA CCCAGTGGTC CTGCCTCTCC TTGTAGTCTG
    GTGAATGAGA CCACTTTGAT TAAATACTCA AGGCTGCCAA CCATAAACAA GCATAGTTTC
    CGGTACTTTG TCTTGGATAA CAGTGTCATC CTGGCAATGC TGGAGCAACC TCTTGGAAAT
    GAACAGAATG ATTTTTTTCC CTCTGTCACT GTGCTGGTTC GAGGAATGTC TGGAAGACTT
    GCTTGGGCAC AACAACTTTG CCTTTTACCC AGAGGAGCAA AAGCAAATCA GAAGCTTTTT
    GTACCTGAAC CTCGTCCAAT TCCTAAAAAT GATGTTGGAT TTAAATATAC TGTCAAACAT
    CGACCATTTC CTGAAGAGGT GGATAAGATT CCTTTTGTGA AAGCAGATCT GAGCATTCCA
    GATTTGCATG AAATTGTCAC TGAAGAATTA GAAGAGAGAC ATGAAAAATT AAGGAATGGC
    ATGGCCCAGC AGATTGCTTA TGAAATACAC CTTGAACAAC AAAGTGAGGA AGAATTGCAG
    AAGAGAAGTT TTCCTGATCC AATTACGGAT TGTAAGCCTC CACCTCCTGC CCAAGAATTC
    CAAACAGCAC GCCTCTTCCT TTCACACTTT GGATTTTTGT CTTTAGAAGC ATTGAAGGAA
    CCTGCAAATA GTCGCCTACC TCCTCACCTT ATTGCACTTG ACTCCACGAT ACCTGGATTT
    TTTGATGACA TTGGATATCT GGATCTCTTG CCGTGTCGTC CCTTTGACAC AGTTTTTATT
    TTCTATATGA AACCAGGTCA GAAAACAAAC CAGGAGATTT TAAAGAATGT GGAATCTTCC
    AGAAATGTTC AGCCACATTT CCTAGAATTT TTGCTCTCCC TTGGCTGGTC AGTAGATGTG
    GGCAGACACC CAGGTTGGAC TGGGCATGTT TCTACCAGTT GGTCTATTAA CAGTTGTGAT
    GATGGTGAAG GACCTGAACA AGATGAAGTG ATTTCCTCTG AAGACATTGG GGCTAGCATT
    TTCAATGGAC AGAAGAAGGT GCTGTATTAT GCTGATGCCC TTACGGAAAT AGCTTTTGTA
    GTTCCTTCTC CTGTGGAGTC TTTAACTGAT TCATTAGAAA GTAACATCTC GGACCAAGAT
    AGTGATTCAA ATATGGATCT TATGCCTGGA ATTCTGAAAC AGCCATCCCT GACACTTGAG
    CTTTTCCCTA ATCACACAGA CAATCTTAAT TCCTCACAGC GGCTTAGTCC CAGTTCCAGA
    ATGAAGAAGC TGCCTCAGGG TCGCCCTGTG CCTCCCCTTG GACCTGAGAC AAGAGTTTCT
    GTAGTCTGGG TGGAACGCTA TGATGATATA GAGAACTTTC CCCTCTCAGA CCTGCTGACA
    GAGATCAGTA CTGGTGTGGA AACTACTGCA AACAGCAGCA CTTCTCTGAG ATCTACCACT
    CTTGAAAAAG AAGTTCCTGT CATCTTTATC CACCCTTTAA ACACTGGATT ATTCCGGATA
    AAAATTCAAG GAGCCACTGG AAAATTTAAC ATGGTCATCC CGCTCGTGGA TGGGATGATT
    GTCAGCAGAC GAGCACTTGG TTTTCTGGTG AGGCAGACTG TAATTAATAT TTGTAGAAGA
    AAGAGACTGG AGAGTGACTC CTACAGTCCA CCACATGTCC GCCGGAAACA GAAAATCACC
    GACATTGTCA ACAAGTACCG GAACAAGCAG TTGGAGCCAG AGTTTTATAC TTCACTTTTC
    CAGGAGGTTG GACTCAAGAA CTGCTCTTCT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005635106.1
    CDS438..4970
    Translation

    Target ORF information:

    RefSeq Version XM_005635049.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Ral GTPase activating protein, beta subunit (non-catalytic) (RALGAPB), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005635049.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATCCAGA ATGATCAGGG CCACACGAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGG CGAGAGGTGG CAAATGCAGT GGTCCATCCC
    CTTGGGCAGG CCTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAGTTTGTT AAAAACTGAC
    AAAGAAGTGA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGTGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTATTGCCG
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC TATATGTTCA AAGTATACTA
    AAACACCTGC AAAATCTTTT TGTACCAAGA CAGGAACAGG GCTCCAGTCA GATCCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTAGCCCGTG AGTCATCCAT CATGGCACGA
    GAAACGTGGG AAGTCTTACT ATTGTTTCTT CTGCAGATTA ATGACATACT TCTAGCCCCG
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTTTGGT TACTAGCTTG TACTCGGTGC TTTCCAACAC CTCCTTATTG GAAAACCGCC
    AAGGAGATGG TGGCAAACTG GAGGCACCAC CCGGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ACCTGTGCCC TCACTTCAAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTCCATTT
    AAAGTTCCTG ATGAAGATGC CAGTCTGATC CCTCCTGAAA TGGATAATGA GTGTGTAGCA
    CAGACATGGT TTCGCTTCTT ACATATGTTA AGTAATCCCG TGGATTTGAG TAACCCGGCC
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTAA ATGTGAGCGG AATGCCACAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTAGTGGA TGCATTCTTA GGCATTTCTA GACCCCGATC AGACAGTGCT
    CCTCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGCA CTGCTGTCAG TACCACCCCA
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC GAAAGTTCAG CACCAGACAT CCTCCACCTC TCCTCTGTCA
    AGTCCCAATC AGACTAGTTC TGAACCCCGG CCACTGCCTG CCCCTCGAAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCAT CTTTTATCCA AATTCTTCTT
    TCTTATAAAT CTTCCATTAC AACACAAGCT AGCATGGAGT TTCGACGGAA AGGGTCACAA
    ATGTCCACAG ACACCATGGT TTCCAATCCT ATGTTTGATG CAAGTGAATT TCCTGATAAC
    TATGAAGCAG GAAGAGCTGA GGCTTGTGGG ACACTGTGTA GGATTTTTTG TAGCAAGAAG
    ACTGGAGAAG AGATTCTGCC AGCTTATTTA TCCAGATTTT ACATGCTTTT AATTCAAGGT
    TTGCAGATAA ATGATTATGT GTGCCATCCT GTCTTGGCCA GCGTTATTCT AAACTCCCCT
    CCTTTGTTCT GCTGTGACTT GAAAGGGATT GATGTTGTGG TTCCTTACTT TATTTCAGCT
    CTTGAAACCA TTTTGCCTGA CAGAGAACTC TCAAAATTCA AAAGCTATGT AAATCCAACA
    GAATTGAGAA GATCTTCCAT TAATATCCTC CTTTCTTTAT TGCCCCTCCC TCATCATTTT
    GGCACAGTCA GATCTGAGGT GGTCCTGGAA GGGAAGTTTA GTAATGATGA CAGCTCTTCA
    TATGATAAAC CAATAACTTT TTTGTCCCTG AAGTTGAGAC TTGTGAATAT ACTAATAGGT
    GCCTTGCAAA CGGAAACGGA CCCCAACAAC ACTCAAATGA TACTAGGGGC AATGTTAAAT
    ATTGTTCAAG ATTCAGCACT TTTAGAAGCC ATTGGTTGCC AAATGGAGAT GGGTGGTGGA
    GAAAATAACC TGAAGAGTCA TAGTCGTACT AATAGTGGTA TTAGTTCAGC AAGTGGTGGA
    AGCACAGAAC CCACAACTCC TGATAGTGAA AGACCTGCTC AAGCTCTCCT AAGGGATTAT
    GGATCGACAG CTCTTAATAC AGATTCAGCT GCTGGGCTCC TGATTCGCAG CATTCATCTT
    GTCACCCAAA GACTCAACTC TCAGTGGCGG CAAGACATGA GCATATCACT GGCAGCGCTG
    GAGCTCCTCT CTGGCCTGGC AAAGGTGAAG GTGATGGTTG ACTCGGGAGA CCGGAAACGA
    GCCATCAGTT CTGTGTGCAG CTACATCGTG TACCAATGTA GTCGGCCAGC TCCTCTTCAC
    TCCCGCGATC TGCACTCCAT GATAGTAGCA GCTTTTCAGT GTCTCTGTGT CTGGCTGACA
    GAGCATCCTG ATATGCTTGA TGAAAAGGAC TGTCTTAAGG AAGTATTGGA GATTGTGGAA
    CTGGGTATTT CAGGAAGTAA GTCCAAGAAC AGTGAGCAAG AGGTCAAGTA CAAAGGAGAT
    AAGGAGCCAA ACCCTGCATC TATGAGGGTA AAGGATGCTG CTGAAGCCAC TCTAACATGT
    ATTATGCAGT TGCTCGGCGC GTTTCCTTCA CCCAGTGGTC CTGCCTCTCC TTGTAGTCTG
    GTGAATGAGA CCACTTTGAT TAAATACTCA AGGCTGCCAA CCATAAACAA GCATAGTTTC
    CGGTACTTTG TCTTGGATAA CAGTGTCATC CTGGCAATGC TGGAGCAACC TCTTGGAAAT
    GAACAGAATG ATTTTTTTCC CTCTGTCACT GTGCTGGTTC GAGGAATGTC TGGAAGACTT
    GCTTGGGCAC AACAACTTTG CCTTTTACCC AGAGGAGCAA AAGCAAATCA GAAGCTTTTT
    GTACCTGAAC CTCGTCCAAT TCCTAAAAAT GATGTTGGAT TTAAATATAC TGTCAAACAT
    CGACCATTTC CTGAAGAGGT GGATAAGATT CCTTTTGTGA AAGCAGATCT GAGCATTCCA
    GATTTGCATG AAATTGTCAC TGAAGAATTA GAAGAGAGAC ATGAAAAATT AAGGAATGGC
    ATGGCCCAGC AGATTGCTTA TGAAATACAC CTTGAACAAC AAAGTGAGGA AGAATTGCAG
    AAGAGAAGTT TTCCTGATCC AATTACGGAT TGTAAGCCTC CACCTCCTGC CCAAGAATTC
    CAAACAGCAC GCCTCTTCCT TTCACACTTT GGATTTTTGT CTTTAGAAGC ATTGAAGGAA
    CCTGCAAATA GTCGCCTACC TCCTCACCTT ATTGCACTTG ACTCCACGAT ACCTGGATTT
    TTTGATGACA TTGGATATCT GGATCTCTTG CCGTGTCGTC CCTTTGACAC AGTTTTTATT
    TTCTATATGA AACCAGGTCA GAAAACAAAC CAGGAGATTT TAAAGAATGT GGAATCTTCC
    AGAAATGTTC AGCCACATTT CCTAGAATTT TTGCTCTCCC TTGGCTGGTC AGTAGATGTG
    GGCAGACACC CAGGTTGGAC TGGGCATGTT TCTACCAGTT GGTCTATTAA CAGTTGTGAT
    GATGGTGAAG GACCTGAACA AGATGAAGTG ATTTCCTCTG AAGACATTGG GGCTAGCATT
    TTCAATGGAC AGAAGAAGGT GCTGTATTAT GCTGATGCCC TTACGGAAAT AGCTTTTGTA
    GTTCCTTCTC CTGTGGAGTC TTTAACTGAT TCATTAGAAA GTAACATCTC GGACCAAGAT
    AGTGATTCAA ATATGGATCT TATGCCTGGA ATTCTGAAAC AGCCATCCCT GACACTTGAG
    CTTTTCCCTA ATCACACAGA CAATCTTAAT TCCTCACAGC GGCTTAGTCC CAGTTCCAGA
    ATGAAGAAGC TGCCTCAGGG TCGCCCTGTG CCTCCCCTTG GACCTGAGAC AAGAGTTTCT
    GTAGTCTGGG TGGAACGCTA TGATGATATA GAGAACTTTC CCCTCTCAGA CCTGCTGACA
    GAGATCAGTA CTGGTGTGGA AACTACTGCA AACAGCAGCA CTTCTCTGAG ATCTACCACT
    CTTGAAAAAG AAGTTCCTGT CATCTTTATC CACCCTTTAA ACACTGGATT ATTCCGGATA
    AAAATTCAAG GAGCCACTGG AAAATTTAAC ATGGTCATCC CGCTCGTGGA TGGGATGATT
    GTCAGCAGAC GAGCACTTGG TTTTCTGGTG AGGCAGACTG TAATTAATAT TTGTAGAAGA
    AAGAGACTGG AGAGTGACTC CTACAGTCCA CCACATGTCC GCCGGAAACA GAAAATCACC
    GACATTGTCA ACAAGTACCG GAACAAGCAG TTGGAGCCAG AGTTTTATAC TTCACTTTTC
    CAGGAGGTTG GACTCAAGAA CTGCTCTTCT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe24888
    Clone ID Related Accession (Same CDS sequence) XM_022409441.1 , XM_005635050.3 , XM_005635049.2 , XM_005635048.2 , XM_005635050.2 , XM_005635049.3 , XM_005635048.3
    Accession Version XM_005635048.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4533bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ral GTPase-activating protein subunit beta isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726101.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005635048.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATCCAGA ATGATCAGGG CCACACGAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGG CGAGAGGTGG CAAATGCAGT GGTCCATCCC
    CTTGGGCAGG CCTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAGTTTGTT AAAAACTGAC
    AAAGAAGTGA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGTGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTATTGCCG
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC TATATGTTCA AAGTATACTA
    AAACACCTGC AAAATCTTTT TGTACCAAGA CAGGAACAGG GCTCCAGTCA GATCCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTAGCCCGTG AGTCATCCAT CATGGCACGA
    GAAACGTGGG AAGTCTTACT ATTGTTTCTT CTGCAGATTA ATGACATACT TCTAGCCCCG
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTTTGGT TACTAGCTTG TACTCGGTGC TTTCCAACAC CTCCTTATTG GAAAACCGCC
    AAGGAGATGG TGGCAAACTG GAGGCACCAC CCGGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ACCTGTGCCC TCACTTCAAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTCCATTT
    AAAGTTCCTG ATGAAGATGC CAGTCTGATC CCTCCTGAAA TGGATAATGA GTGTGTAGCA
    CAGACATGGT TTCGCTTCTT ACATATGTTA AGTAATCCCG TGGATTTGAG TAACCCGGCC
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTAA ATGTGAGCGG AATGCCACAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTAGTGGA TGCATTCTTA GGCATTTCTA GACCCCGATC AGACAGTGCT
    CCTCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGCA CTGCTGTCAG TACCACCCCA
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC GAAAGTTCAG CACCAGACAT CCTCCACCTC TCCTCTGTCA
    AGTCCCAATC AGACTAGTTC TGAACCCCGG CCACTGCCTG CCCCTCGAAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCAT CTTTTATCCA AATTCTTCTT
    TCTTATAAAT CTTCCATTAC AACACAAGCT AGCATGGAGT TTCGACGGAA AGGGTCACAA
    ATGTCCACAG ACACCATGGT TTCCAATCCT ATGTTTGATG CAAGTGAATT TCCTGATAAC
    TATGAAGCAG GAAGAGCTGA GGCTTGTGGG ACACTGTGTA GGATTTTTTG TAGCAAGAAG
    ACTGGAGAAG AGATTCTGCC AGCTTATTTA TCCAGATTTT ACATGCTTTT AATTCAAGGT
    TTGCAGATAA ATGATTATGT GTGCCATCCT GTCTTGGCCA GCGTTATTCT AAACTCCCCT
    CCTTTGTTCT GCTGTGACTT GAAAGGGATT GATGTTGTGG TTCCTTACTT TATTTCAGCT
    CTTGAAACCA TTTTGCCTGA CAGAGAACTC TCAAAATTCA AAAGCTATGT AAATCCAACA
    GAATTGAGAA GATCTTCCAT TAATATCCTC CTTTCTTTAT TGCCCCTCCC TCATCATTTT
    GGCACAGTCA GATCTGAGGT GGTCCTGGAA GGGAAGTTTA GTAATGATGA CAGCTCTTCA
    TATGATAAAC CAATAACTTT TTTGTCCCTG AAGTTGAGAC TTGTGAATAT ACTAATAGGT
    GCCTTGCAAA CGGAAACGGA CCCCAACAAC ACTCAAATGA TACTAGGGGC AATGTTAAAT
    ATTGTTCAAG ATTCAGCACT TTTAGAAGCC ATTGGTTGCC AAATGGAGAT GGGTGGTGGA
    GAAAATAACC TGAAGAGTCA TAGTCGTACT AATAGTGGTA TTAGTTCAGC AAGTGGTGGA
    AGCACAGAAC CCACAACTCC TGATAGTGAA AGACCTGCTC AAGCTCTCCT AAGGGATTAT
    GGATCGACAG CTCTTAATAC AGATTCAGCT GCTGGGCTCC TGATTCGCAG CATTCATCTT
    GTCACCCAAA GACTCAACTC TCAGTGGCGG CAAGACATGA GCATATCACT GGCAGCGCTG
    GAGCTCCTCT CTGGCCTGGC AAAGGTGAAG GTGATGGTTG ACTCGGGAGA CCGGAAACGA
    GCCATCAGTT CTGTGTGCAG CTACATCGTG TACCAATGTA GTCGGCCAGC TCCTCTTCAC
    TCCCGCGATC TGCACTCCAT GATAGTAGCA GCTTTTCAGT GTCTCTGTGT CTGGCTGACA
    GAGCATCCTG ATATGCTTGA TGAAAAGGAC TGTCTTAAGG AAGTATTGGA GATTGTGGAA
    CTGGGTATTT CAGGAAGTAA GTCCAAGAAC AGTGAGCAAG AGGTCAAGTA CAAAGGAGAT
    AAGGAGCCAA ACCCTGCATC TATGAGGGTA AAGGATGCTG CTGAAGCCAC TCTAACATGT
    ATTATGCAGT TGCTCGGCGC GTTTCCTTCA CCCAGTGGTC CTGCCTCTCC TTGTAGTCTG
    GTGAATGAGA CCACTTTGAT TAAATACTCA AGGCTGCCAA CCATAAACAA GCATAGTTTC
    CGGTACTTTG TCTTGGATAA CAGTGTCATC CTGGCAATGC TGGAGCAACC TCTTGGAAAT
    GAACAGAATG ATTTTTTTCC CTCTGTCACT GTGCTGGTTC GAGGAATGTC TGGAAGACTT
    GCTTGGGCAC AACAACTTTG CCTTTTACCC AGAGGAGCAA AAGCAAATCA GAAGCTTTTT
    GTACCTGAAC CTCGTCCAAT TCCTAAAAAT GATGTTGGAT TTAAATATAC TGTCAAACAT
    CGACCATTTC CTGAAGAGGT GGATAAGATT CCTTTTGTGA AAGCAGATCT GAGCATTCCA
    GATTTGCATG AAATTGTCAC TGAAGAATTA GAAGAGAGAC ATGAAAAATT AAGGAATGGC
    ATGGCCCAGC AGATTGCTTA TGAAATACAC CTTGAACAAC AAAGTGAGGA AGAATTGCAG
    AAGAGAAGTT TTCCTGATCC AATTACGGAT TGTAAGCCTC CACCTCCTGC CCAAGAATTC
    CAAACAGCAC GCCTCTTCCT TTCACACTTT GGATTTTTGT CTTTAGAAGC ATTGAAGGAA
    CCTGCAAATA GTCGCCTACC TCCTCACCTT ATTGCACTTG ACTCCACGAT ACCTGGATTT
    TTTGATGACA TTGGATATCT GGATCTCTTG CCGTGTCGTC CCTTTGACAC AGTTTTTATT
    TTCTATATGA AACCAGGTCA GAAAACAAAC CAGGAGATTT TAAAGAATGT GGAATCTTCC
    AGAAATGTTC AGCCACATTT CCTAGAATTT TTGCTCTCCC TTGGCTGGTC AGTAGATGTG
    GGCAGACACC CAGGTTGGAC TGGGCATGTT TCTACCAGTT GGTCTATTAA CAGTTGTGAT
    GATGGTGAAG GACCTGAACA AGATGAAGTG ATTTCCTCTG AAGACATTGG GGCTAGCATT
    TTCAATGGAC AGAAGAAGGT GCTGTATTAT GCTGATGCCC TTACGGAAAT AGCTTTTGTA
    GTTCCTTCTC CTGTGGAGTC TTTAACTGAT TCATTAGAAA GTAACATCTC GGACCAAGAT
    AGTGATTCAA ATATGGATCT TATGCCTGGA ATTCTGAAAC AGCCATCCCT GACACTTGAG
    CTTTTCCCTA ATCACACAGA CAATCTTAAT TCCTCACAGC GGCTTAGTCC CAGTTCCAGA
    ATGAAGAAGC TGCCTCAGGG TCGCCCTGTG CCTCCCCTTG GACCTGAGAC AAGAGTTTCT
    GTAGTCTGGG TGGAACGCTA TGATGATATA GAGAACTTTC CCCTCTCAGA CCTGCTGACA
    GAGATCAGTA CTGGTGTGGA AACTACTGCA AACAGCAGCA CTTCTCTGAG ATCTACCACT
    CTTGAAAAAG AAGTTCCTGT CATCTTTATC CACCCTTTAA ACACTGGATT ATTCCGGATA
    AAAATTCAAG GAGCCACTGG AAAATTTAAC ATGGTCATCC CGCTCGTGGA TGGGATGATT
    GTCAGCAGAC GAGCACTTGG TTTTCTGGTG AGGCAGACTG TAATTAATAT TTGTAGAAGA
    AAGAGACTGG AGAGTGACTC CTACAGTCCA CCACATGTCC GCCGGAAACA GAAAATCACC
    GACATTGTCA ACAAGTACCG GAACAAGCAG TTGGAGCCAG AGTTTTATAC TTCACTTTTC
    CAGGAGGTTG GACTCAAGAA CTGCTCTTCT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005635105.1
    CDS333..4865
    Translation

    Target ORF information:

    RefSeq Version XM_005635048.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Ral GTPase activating protein, beta subunit (non-catalytic) (RALGAPB), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005635048.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATCCAGA ATGATCAGGG CCACACGAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGG CGAGAGGTGG CAAATGCAGT GGTCCATCCC
    CTTGGGCAGG CCTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAGTTTGTT AAAAACTGAC
    AAAGAAGTGA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGTGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTATTGCCG
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC TATATGTTCA AAGTATACTA
    AAACACCTGC AAAATCTTTT TGTACCAAGA CAGGAACAGG GCTCCAGTCA GATCCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTAGCCCGTG AGTCATCCAT CATGGCACGA
    GAAACGTGGG AAGTCTTACT ATTGTTTCTT CTGCAGATTA ATGACATACT TCTAGCCCCG
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTTTGGT TACTAGCTTG TACTCGGTGC TTTCCAACAC CTCCTTATTG GAAAACCGCC
    AAGGAGATGG TGGCAAACTG GAGGCACCAC CCGGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ACCTGTGCCC TCACTTCAAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTCCATTT
    AAAGTTCCTG ATGAAGATGC CAGTCTGATC CCTCCTGAAA TGGATAATGA GTGTGTAGCA
    CAGACATGGT TTCGCTTCTT ACATATGTTA AGTAATCCCG TGGATTTGAG TAACCCGGCC
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTAA ATGTGAGCGG AATGCCACAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTAGTGGA TGCATTCTTA GGCATTTCTA GACCCCGATC AGACAGTGCT
    CCTCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGCA CTGCTGTCAG TACCACCCCA
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC GAAAGTTCAG CACCAGACAT CCTCCACCTC TCCTCTGTCA
    AGTCCCAATC AGACTAGTTC TGAACCCCGG CCACTGCCTG CCCCTCGAAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCAT CTTTTATCCA AATTCTTCTT
    TCTTATAAAT CTTCCATTAC AACACAAGCT AGCATGGAGT TTCGACGGAA AGGGTCACAA
    ATGTCCACAG ACACCATGGT TTCCAATCCT ATGTTTGATG CAAGTGAATT TCCTGATAAC
    TATGAAGCAG GAAGAGCTGA GGCTTGTGGG ACACTGTGTA GGATTTTTTG TAGCAAGAAG
    ACTGGAGAAG AGATTCTGCC AGCTTATTTA TCCAGATTTT ACATGCTTTT AATTCAAGGT
    TTGCAGATAA ATGATTATGT GTGCCATCCT GTCTTGGCCA GCGTTATTCT AAACTCCCCT
    CCTTTGTTCT GCTGTGACTT GAAAGGGATT GATGTTGTGG TTCCTTACTT TATTTCAGCT
    CTTGAAACCA TTTTGCCTGA CAGAGAACTC TCAAAATTCA AAAGCTATGT AAATCCAACA
    GAATTGAGAA GATCTTCCAT TAATATCCTC CTTTCTTTAT TGCCCCTCCC TCATCATTTT
    GGCACAGTCA GATCTGAGGT GGTCCTGGAA GGGAAGTTTA GTAATGATGA CAGCTCTTCA
    TATGATAAAC CAATAACTTT TTTGTCCCTG AAGTTGAGAC TTGTGAATAT ACTAATAGGT
    GCCTTGCAAA CGGAAACGGA CCCCAACAAC ACTCAAATGA TACTAGGGGC AATGTTAAAT
    ATTGTTCAAG ATTCAGCACT TTTAGAAGCC ATTGGTTGCC AAATGGAGAT GGGTGGTGGA
    GAAAATAACC TGAAGAGTCA TAGTCGTACT AATAGTGGTA TTAGTTCAGC AAGTGGTGGA
    AGCACAGAAC CCACAACTCC TGATAGTGAA AGACCTGCTC AAGCTCTCCT AAGGGATTAT
    GGATCGACAG CTCTTAATAC AGATTCAGCT GCTGGGCTCC TGATTCGCAG CATTCATCTT
    GTCACCCAAA GACTCAACTC TCAGTGGCGG CAAGACATGA GCATATCACT GGCAGCGCTG
    GAGCTCCTCT CTGGCCTGGC AAAGGTGAAG GTGATGGTTG ACTCGGGAGA CCGGAAACGA
    GCCATCAGTT CTGTGTGCAG CTACATCGTG TACCAATGTA GTCGGCCAGC TCCTCTTCAC
    TCCCGCGATC TGCACTCCAT GATAGTAGCA GCTTTTCAGT GTCTCTGTGT CTGGCTGACA
    GAGCATCCTG ATATGCTTGA TGAAAAGGAC TGTCTTAAGG AAGTATTGGA GATTGTGGAA
    CTGGGTATTT CAGGAAGTAA GTCCAAGAAC AGTGAGCAAG AGGTCAAGTA CAAAGGAGAT
    AAGGAGCCAA ACCCTGCATC TATGAGGGTA AAGGATGCTG CTGAAGCCAC TCTAACATGT
    ATTATGCAGT TGCTCGGCGC GTTTCCTTCA CCCAGTGGTC CTGCCTCTCC TTGTAGTCTG
    GTGAATGAGA CCACTTTGAT TAAATACTCA AGGCTGCCAA CCATAAACAA GCATAGTTTC
    CGGTACTTTG TCTTGGATAA CAGTGTCATC CTGGCAATGC TGGAGCAACC TCTTGGAAAT
    GAACAGAATG ATTTTTTTCC CTCTGTCACT GTGCTGGTTC GAGGAATGTC TGGAAGACTT
    GCTTGGGCAC AACAACTTTG CCTTTTACCC AGAGGAGCAA AAGCAAATCA GAAGCTTTTT
    GTACCTGAAC CTCGTCCAAT TCCTAAAAAT GATGTTGGAT TTAAATATAC TGTCAAACAT
    CGACCATTTC CTGAAGAGGT GGATAAGATT CCTTTTGTGA AAGCAGATCT GAGCATTCCA
    GATTTGCATG AAATTGTCAC TGAAGAATTA GAAGAGAGAC ATGAAAAATT AAGGAATGGC
    ATGGCCCAGC AGATTGCTTA TGAAATACAC CTTGAACAAC AAAGTGAGGA AGAATTGCAG
    AAGAGAAGTT TTCCTGATCC AATTACGGAT TGTAAGCCTC CACCTCCTGC CCAAGAATTC
    CAAACAGCAC GCCTCTTCCT TTCACACTTT GGATTTTTGT CTTTAGAAGC ATTGAAGGAA
    CCTGCAAATA GTCGCCTACC TCCTCACCTT ATTGCACTTG ACTCCACGAT ACCTGGATTT
    TTTGATGACA TTGGATATCT GGATCTCTTG CCGTGTCGTC CCTTTGACAC AGTTTTTATT
    TTCTATATGA AACCAGGTCA GAAAACAAAC CAGGAGATTT TAAAGAATGT GGAATCTTCC
    AGAAATGTTC AGCCACATTT CCTAGAATTT TTGCTCTCCC TTGGCTGGTC AGTAGATGTG
    GGCAGACACC CAGGTTGGAC TGGGCATGTT TCTACCAGTT GGTCTATTAA CAGTTGTGAT
    GATGGTGAAG GACCTGAACA AGATGAAGTG ATTTCCTCTG AAGACATTGG GGCTAGCATT
    TTCAATGGAC AGAAGAAGGT GCTGTATTAT GCTGATGCCC TTACGGAAAT AGCTTTTGTA
    GTTCCTTCTC CTGTGGAGTC TTTAACTGAT TCATTAGAAA GTAACATCTC GGACCAAGAT
    AGTGATTCAA ATATGGATCT TATGCCTGGA ATTCTGAAAC AGCCATCCCT GACACTTGAG
    CTTTTCCCTA ATCACACAGA CAATCTTAAT TCCTCACAGC GGCTTAGTCC CAGTTCCAGA
    ATGAAGAAGC TGCCTCAGGG TCGCCCTGTG CCTCCCCTTG GACCTGAGAC AAGAGTTTCT
    GTAGTCTGGG TGGAACGCTA TGATGATATA GAGAACTTTC CCCTCTCAGA CCTGCTGACA
    GAGATCAGTA CTGGTGTGGA AACTACTGCA AACAGCAGCA CTTCTCTGAG ATCTACCACT
    CTTGAAAAAG AAGTTCCTGT CATCTTTATC CACCCTTTAA ACACTGGATT ATTCCGGATA
    AAAATTCAAG GAGCCACTGG AAAATTTAAC ATGGTCATCC CGCTCGTGGA TGGGATGATT
    GTCAGCAGAC GAGCACTTGG TTTTCTGGTG AGGCAGACTG TAATTAATAT TTGTAGAAGA
    AAGAGACTGG AGAGTGACTC CTACAGTCCA CCACATGTCC GCCGGAAACA GAAAATCACC
    GACATTGTCA ACAAGTACCG GAACAAGCAG TTGGAGCCAG AGTTTTATAC TTCACTTTTC
    CAGGAGGTTG GACTCAAGAA CTGCTCTTCT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe24888
    Clone ID Related Accession (Same CDS sequence) XM_022409441.1 , XM_005635050.3 , XM_005635049.2 , XM_005635048.2 , XM_005635050.2 , XM_005635049.3 , XM_005635048.3
    Accession Version XM_005635050.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4533bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ral GTPase-activating protein subunit beta isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726101.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005635050.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATCCAGA ATGATCAGGG CCACACGAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGG CGAGAGGTGG CAAATGCAGT GGTCCATCCC
    CTTGGGCAGG CCTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAGTTTGTT AAAAACTGAC
    AAAGAAGTGA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGTGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTATTGCCG
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC TATATGTTCA AAGTATACTA
    AAACACCTGC AAAATCTTTT TGTACCAAGA CAGGAACAGG GCTCCAGTCA GATCCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTAGCCCGTG AGTCATCCAT CATGGCACGA
    GAAACGTGGG AAGTCTTACT ATTGTTTCTT CTGCAGATTA ATGACATACT TCTAGCCCCG
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTTTGGT TACTAGCTTG TACTCGGTGC TTTCCAACAC CTCCTTATTG GAAAACCGCC
    AAGGAGATGG TGGCAAACTG GAGGCACCAC CCGGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ACCTGTGCCC TCACTTCAAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTCCATTT
    AAAGTTCCTG ATGAAGATGC CAGTCTGATC CCTCCTGAAA TGGATAATGA GTGTGTAGCA
    CAGACATGGT TTCGCTTCTT ACATATGTTA AGTAATCCCG TGGATTTGAG TAACCCGGCC
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTAA ATGTGAGCGG AATGCCACAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTAGTGGA TGCATTCTTA GGCATTTCTA GACCCCGATC AGACAGTGCT
    CCTCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGCA CTGCTGTCAG TACCACCCCA
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC GAAAGTTCAG CACCAGACAT CCTCCACCTC TCCTCTGTCA
    AGTCCCAATC AGACTAGTTC TGAACCCCGG CCACTGCCTG CCCCTCGAAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCAT CTTTTATCCA AATTCTTCTT
    TCTTATAAAT CTTCCATTAC AACACAAGCT AGCATGGAGT TTCGACGGAA AGGGTCACAA
    ATGTCCACAG ACACCATGGT TTCCAATCCT ATGTTTGATG CAAGTGAATT TCCTGATAAC
    TATGAAGCAG GAAGAGCTGA GGCTTGTGGG ACACTGTGTA GGATTTTTTG TAGCAAGAAG
    ACTGGAGAAG AGATTCTGCC AGCTTATTTA TCCAGATTTT ACATGCTTTT AATTCAAGGT
    TTGCAGATAA ATGATTATGT GTGCCATCCT GTCTTGGCCA GCGTTATTCT AAACTCCCCT
    CCTTTGTTCT GCTGTGACTT GAAAGGGATT GATGTTGTGG TTCCTTACTT TATTTCAGCT
    CTTGAAACCA TTTTGCCTGA CAGAGAACTC TCAAAATTCA AAAGCTATGT AAATCCAACA
    GAATTGAGAA GATCTTCCAT TAATATCCTC CTTTCTTTAT TGCCCCTCCC TCATCATTTT
    GGCACAGTCA GATCTGAGGT GGTCCTGGAA GGGAAGTTTA GTAATGATGA CAGCTCTTCA
    TATGATAAAC CAATAACTTT TTTGTCCCTG AAGTTGAGAC TTGTGAATAT ACTAATAGGT
    GCCTTGCAAA CGGAAACGGA CCCCAACAAC ACTCAAATGA TACTAGGGGC AATGTTAAAT
    ATTGTTCAAG ATTCAGCACT TTTAGAAGCC ATTGGTTGCC AAATGGAGAT GGGTGGTGGA
    GAAAATAACC TGAAGAGTCA TAGTCGTACT AATAGTGGTA TTAGTTCAGC AAGTGGTGGA
    AGCACAGAAC CCACAACTCC TGATAGTGAA AGACCTGCTC AAGCTCTCCT AAGGGATTAT
    GGATCGACAG CTCTTAATAC AGATTCAGCT GCTGGGCTCC TGATTCGCAG CATTCATCTT
    GTCACCCAAA GACTCAACTC TCAGTGGCGG CAAGACATGA GCATATCACT GGCAGCGCTG
    GAGCTCCTCT CTGGCCTGGC AAAGGTGAAG GTGATGGTTG ACTCGGGAGA CCGGAAACGA
    GCCATCAGTT CTGTGTGCAG CTACATCGTG TACCAATGTA GTCGGCCAGC TCCTCTTCAC
    TCCCGCGATC TGCACTCCAT GATAGTAGCA GCTTTTCAGT GTCTCTGTGT CTGGCTGACA
    GAGCATCCTG ATATGCTTGA TGAAAAGGAC TGTCTTAAGG AAGTATTGGA GATTGTGGAA
    CTGGGTATTT CAGGAAGTAA GTCCAAGAAC AGTGAGCAAG AGGTCAAGTA CAAAGGAGAT
    AAGGAGCCAA ACCCTGCATC TATGAGGGTA AAGGATGCTG CTGAAGCCAC TCTAACATGT
    ATTATGCAGT TGCTCGGCGC GTTTCCTTCA CCCAGTGGTC CTGCCTCTCC TTGTAGTCTG
    GTGAATGAGA CCACTTTGAT TAAATACTCA AGGCTGCCAA CCATAAACAA GCATAGTTTC
    CGGTACTTTG TCTTGGATAA CAGTGTCATC CTGGCAATGC TGGAGCAACC TCTTGGAAAT
    GAACAGAATG ATTTTTTTCC CTCTGTCACT GTGCTGGTTC GAGGAATGTC TGGAAGACTT
    GCTTGGGCAC AACAACTTTG CCTTTTACCC AGAGGAGCAA AAGCAAATCA GAAGCTTTTT
    GTACCTGAAC CTCGTCCAAT TCCTAAAAAT GATGTTGGAT TTAAATATAC TGTCAAACAT
    CGACCATTTC CTGAAGAGGT GGATAAGATT CCTTTTGTGA AAGCAGATCT GAGCATTCCA
    GATTTGCATG AAATTGTCAC TGAAGAATTA GAAGAGAGAC ATGAAAAATT AAGGAATGGC
    ATGGCCCAGC AGATTGCTTA TGAAATACAC CTTGAACAAC AAAGTGAGGA AGAATTGCAG
    AAGAGAAGTT TTCCTGATCC AATTACGGAT TGTAAGCCTC CACCTCCTGC CCAAGAATTC
    CAAACAGCAC GCCTCTTCCT TTCACACTTT GGATTTTTGT CTTTAGAAGC ATTGAAGGAA
    CCTGCAAATA GTCGCCTACC TCCTCACCTT ATTGCACTTG ACTCCACGAT ACCTGGATTT
    TTTGATGACA TTGGATATCT GGATCTCTTG CCGTGTCGTC CCTTTGACAC AGTTTTTATT
    TTCTATATGA AACCAGGTCA GAAAACAAAC CAGGAGATTT TAAAGAATGT GGAATCTTCC
    AGAAATGTTC AGCCACATTT CCTAGAATTT TTGCTCTCCC TTGGCTGGTC AGTAGATGTG
    GGCAGACACC CAGGTTGGAC TGGGCATGTT TCTACCAGTT GGTCTATTAA CAGTTGTGAT
    GATGGTGAAG GACCTGAACA AGATGAAGTG ATTTCCTCTG AAGACATTGG GGCTAGCATT
    TTCAATGGAC AGAAGAAGGT GCTGTATTAT GCTGATGCCC TTACGGAAAT AGCTTTTGTA
    GTTCCTTCTC CTGTGGAGTC TTTAACTGAT TCATTAGAAA GTAACATCTC GGACCAAGAT
    AGTGATTCAA ATATGGATCT TATGCCTGGA ATTCTGAAAC AGCCATCCCT GACACTTGAG
    CTTTTCCCTA ATCACACAGA CAATCTTAAT TCCTCACAGC GGCTTAGTCC CAGTTCCAGA
    ATGAAGAAGC TGCCTCAGGG TCGCCCTGTG CCTCCCCTTG GACCTGAGAC AAGAGTTTCT
    GTAGTCTGGG TGGAACGCTA TGATGATATA GAGAACTTTC CCCTCTCAGA CCTGCTGACA
    GAGATCAGTA CTGGTGTGGA AACTACTGCA AACAGCAGCA CTTCTCTGAG ATCTACCACT
    CTTGAAAAAG AAGTTCCTGT CATCTTTATC CACCCTTTAA ACACTGGATT ATTCCGGATA
    AAAATTCAAG GAGCCACTGG AAAATTTAAC ATGGTCATCC CGCTCGTGGA TGGGATGATT
    GTCAGCAGAC GAGCACTTGG TTTTCTGGTG AGGCAGACTG TAATTAATAT TTGTAGAAGA
    AAGAGACTGG AGAGTGACTC CTACAGTCCA CCACATGTCC GCCGGAAACA GAAAATCACC
    GACATTGTCA ACAAGTACCG GAACAAGCAG TTGGAGCCAG AGTTTTATAC TTCACTTTTC
    CAGGAGGTTG GACTCAAGAA CTGCTCTTCT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005635107.1
    CDS328..4860
    Translation

    Target ORF information:

    RefSeq Version XM_005635050.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Ral GTPase activating protein, beta subunit (non-catalytic) (RALGAPB), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005635050.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATCCAGA ATGATCAGGG CCACACGAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGG CGAGAGGTGG CAAATGCAGT GGTCCATCCC
    CTTGGGCAGG CCTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAGTTTGTT AAAAACTGAC
    AAAGAAGTGA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGTGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTATTGCCG
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC TATATGTTCA AAGTATACTA
    AAACACCTGC AAAATCTTTT TGTACCAAGA CAGGAACAGG GCTCCAGTCA GATCCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTAGCCCGTG AGTCATCCAT CATGGCACGA
    GAAACGTGGG AAGTCTTACT ATTGTTTCTT CTGCAGATTA ATGACATACT TCTAGCCCCG
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTTTGGT TACTAGCTTG TACTCGGTGC TTTCCAACAC CTCCTTATTG GAAAACCGCC
    AAGGAGATGG TGGCAAACTG GAGGCACCAC CCGGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ACCTGTGCCC TCACTTCAAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTCCATTT
    AAAGTTCCTG ATGAAGATGC CAGTCTGATC CCTCCTGAAA TGGATAATGA GTGTGTAGCA
    CAGACATGGT TTCGCTTCTT ACATATGTTA AGTAATCCCG TGGATTTGAG TAACCCGGCC
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTAA ATGTGAGCGG AATGCCACAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTAGTGGA TGCATTCTTA GGCATTTCTA GACCCCGATC AGACAGTGCT
    CCTCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGCA CTGCTGTCAG TACCACCCCA
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC GAAAGTTCAG CACCAGACAT CCTCCACCTC TCCTCTGTCA
    AGTCCCAATC AGACTAGTTC TGAACCCCGG CCACTGCCTG CCCCTCGAAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCAT CTTTTATCCA AATTCTTCTT
    TCTTATAAAT CTTCCATTAC AACACAAGCT AGCATGGAGT TTCGACGGAA AGGGTCACAA
    ATGTCCACAG ACACCATGGT TTCCAATCCT ATGTTTGATG CAAGTGAATT TCCTGATAAC
    TATGAAGCAG GAAGAGCTGA GGCTTGTGGG ACACTGTGTA GGATTTTTTG TAGCAAGAAG
    ACTGGAGAAG AGATTCTGCC AGCTTATTTA TCCAGATTTT ACATGCTTTT AATTCAAGGT
    TTGCAGATAA ATGATTATGT GTGCCATCCT GTCTTGGCCA GCGTTATTCT AAACTCCCCT
    CCTTTGTTCT GCTGTGACTT GAAAGGGATT GATGTTGTGG TTCCTTACTT TATTTCAGCT
    CTTGAAACCA TTTTGCCTGA CAGAGAACTC TCAAAATTCA AAAGCTATGT AAATCCAACA
    GAATTGAGAA GATCTTCCAT TAATATCCTC CTTTCTTTAT TGCCCCTCCC TCATCATTTT
    GGCACAGTCA GATCTGAGGT GGTCCTGGAA GGGAAGTTTA GTAATGATGA CAGCTCTTCA
    TATGATAAAC CAATAACTTT TTTGTCCCTG AAGTTGAGAC TTGTGAATAT ACTAATAGGT
    GCCTTGCAAA CGGAAACGGA CCCCAACAAC ACTCAAATGA TACTAGGGGC AATGTTAAAT
    ATTGTTCAAG ATTCAGCACT TTTAGAAGCC ATTGGTTGCC AAATGGAGAT GGGTGGTGGA
    GAAAATAACC TGAAGAGTCA TAGTCGTACT AATAGTGGTA TTAGTTCAGC AAGTGGTGGA
    AGCACAGAAC CCACAACTCC TGATAGTGAA AGACCTGCTC AAGCTCTCCT AAGGGATTAT
    GGATCGACAG CTCTTAATAC AGATTCAGCT GCTGGGCTCC TGATTCGCAG CATTCATCTT
    GTCACCCAAA GACTCAACTC TCAGTGGCGG CAAGACATGA GCATATCACT GGCAGCGCTG
    GAGCTCCTCT CTGGCCTGGC AAAGGTGAAG GTGATGGTTG ACTCGGGAGA CCGGAAACGA
    GCCATCAGTT CTGTGTGCAG CTACATCGTG TACCAATGTA GTCGGCCAGC TCCTCTTCAC
    TCCCGCGATC TGCACTCCAT GATAGTAGCA GCTTTTCAGT GTCTCTGTGT CTGGCTGACA
    GAGCATCCTG ATATGCTTGA TGAAAAGGAC TGTCTTAAGG AAGTATTGGA GATTGTGGAA
    CTGGGTATTT CAGGAAGTAA GTCCAAGAAC AGTGAGCAAG AGGTCAAGTA CAAAGGAGAT
    AAGGAGCCAA ACCCTGCATC TATGAGGGTA AAGGATGCTG CTGAAGCCAC TCTAACATGT
    ATTATGCAGT TGCTCGGCGC GTTTCCTTCA CCCAGTGGTC CTGCCTCTCC TTGTAGTCTG
    GTGAATGAGA CCACTTTGAT TAAATACTCA AGGCTGCCAA CCATAAACAA GCATAGTTTC
    CGGTACTTTG TCTTGGATAA CAGTGTCATC CTGGCAATGC TGGAGCAACC TCTTGGAAAT
    GAACAGAATG ATTTTTTTCC CTCTGTCACT GTGCTGGTTC GAGGAATGTC TGGAAGACTT
    GCTTGGGCAC AACAACTTTG CCTTTTACCC AGAGGAGCAA AAGCAAATCA GAAGCTTTTT
    GTACCTGAAC CTCGTCCAAT TCCTAAAAAT GATGTTGGAT TTAAATATAC TGTCAAACAT
    CGACCATTTC CTGAAGAGGT GGATAAGATT CCTTTTGTGA AAGCAGATCT GAGCATTCCA
    GATTTGCATG AAATTGTCAC TGAAGAATTA GAAGAGAGAC ATGAAAAATT AAGGAATGGC
    ATGGCCCAGC AGATTGCTTA TGAAATACAC CTTGAACAAC AAAGTGAGGA AGAATTGCAG
    AAGAGAAGTT TTCCTGATCC AATTACGGAT TGTAAGCCTC CACCTCCTGC CCAAGAATTC
    CAAACAGCAC GCCTCTTCCT TTCACACTTT GGATTTTTGT CTTTAGAAGC ATTGAAGGAA
    CCTGCAAATA GTCGCCTACC TCCTCACCTT ATTGCACTTG ACTCCACGAT ACCTGGATTT
    TTTGATGACA TTGGATATCT GGATCTCTTG CCGTGTCGTC CCTTTGACAC AGTTTTTATT
    TTCTATATGA AACCAGGTCA GAAAACAAAC CAGGAGATTT TAAAGAATGT GGAATCTTCC
    AGAAATGTTC AGCCACATTT CCTAGAATTT TTGCTCTCCC TTGGCTGGTC AGTAGATGTG
    GGCAGACACC CAGGTTGGAC TGGGCATGTT TCTACCAGTT GGTCTATTAA CAGTTGTGAT
    GATGGTGAAG GACCTGAACA AGATGAAGTG ATTTCCTCTG AAGACATTGG GGCTAGCATT
    TTCAATGGAC AGAAGAAGGT GCTGTATTAT GCTGATGCCC TTACGGAAAT AGCTTTTGTA
    GTTCCTTCTC CTGTGGAGTC TTTAACTGAT TCATTAGAAA GTAACATCTC GGACCAAGAT
    AGTGATTCAA ATATGGATCT TATGCCTGGA ATTCTGAAAC AGCCATCCCT GACACTTGAG
    CTTTTCCCTA ATCACACAGA CAATCTTAAT TCCTCACAGC GGCTTAGTCC CAGTTCCAGA
    ATGAAGAAGC TGCCTCAGGG TCGCCCTGTG CCTCCCCTTG GACCTGAGAC AAGAGTTTCT
    GTAGTCTGGG TGGAACGCTA TGATGATATA GAGAACTTTC CCCTCTCAGA CCTGCTGACA
    GAGATCAGTA CTGGTGTGGA AACTACTGCA AACAGCAGCA CTTCTCTGAG ATCTACCACT
    CTTGAAAAAG AAGTTCCTGT CATCTTTATC CACCCTTTAA ACACTGGATT ATTCCGGATA
    AAAATTCAAG GAGCCACTGG AAAATTTAAC ATGGTCATCC CGCTCGTGGA TGGGATGATT
    GTCAGCAGAC GAGCACTTGG TTTTCTGGTG AGGCAGACTG TAATTAATAT TTGTAGAAGA
    AAGAGACTGG AGAGTGACTC CTACAGTCCA CCACATGTCC GCCGGAAACA GAAAATCACC
    GACATTGTCA ACAAGTACCG GAACAAGCAG TTGGAGCCAG AGTTTTATAC TTCACTTTTC
    CAGGAGGTTG GACTCAAGAA CTGCTCTTCT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe24888
    Clone ID Related Accession (Same CDS sequence) XM_022409441.1 , XM_005635050.3 , XM_005635049.2 , XM_005635048.2 , XM_005635050.2 , XM_005635049.3 , XM_005635048.3
    Accession Version XM_005635049.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4533bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ral GTPase-activating protein subunit beta isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005635049.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATCCAGA ATGATCAGGG CCACACGAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGG CGAGAGGTGG CAAATGCAGT GGTCCATCCC
    CTTGGGCAGG CCTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAGTTTGTT AAAAACTGAC
    AAAGAAGTGA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGTGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTATTGCCG
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC TATATGTTCA AAGTATACTA
    AAACACCTGC AAAATCTTTT TGTACCAAGA CAGGAACAGG GCTCCAGTCA GATCCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTAGCCCGTG AGTCATCCAT CATGGCACGA
    GAAACGTGGG AAGTCTTACT ATTGTTTCTT CTGCAGATTA ATGACATACT TCTAGCCCCG
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTTTGGT TACTAGCTTG TACTCGGTGC TTTCCAACAC CTCCTTATTG GAAAACCGCC
    AAGGAGATGG TGGCAAACTG GAGGCACCAC CCGGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ACCTGTGCCC TCACTTCAAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTCCATTT
    AAAGTTCCTG ATGAAGATGC CAGTCTGATC CCTCCTGAAA TGGATAATGA GTGTGTAGCA
    CAGACATGGT TTCGCTTCTT ACATATGTTA AGTAATCCCG TGGATTTGAG TAACCCGGCC
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTAA ATGTGAGCGG AATGCCACAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTAGTGGA TGCATTCTTA GGCATTTCTA GACCCCGATC AGACAGTGCT
    CCTCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGCA CTGCTGTCAG TACCACCCCA
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC GAAAGTTCAG CACCAGACAT CCTCCACCTC TCCTCTGTCA
    AGTCCCAATC AGACTAGTTC TGAACCCCGG CCACTGCCTG CCCCTCGAAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCAT CTTTTATCCA AATTCTTCTT
    TCTTATAAAT CTTCCATTAC AACACAAGCT AGCATGGAGT TTCGACGGAA AGGGTCACAA
    ATGTCCACAG ACACCATGGT TTCCAATCCT ATGTTTGATG CAAGTGAATT TCCTGATAAC
    TATGAAGCAG GAAGAGCTGA GGCTTGTGGG ACACTGTGTA GGATTTTTTG TAGCAAGAAG
    ACTGGAGAAG AGATTCTGCC AGCTTATTTA TCCAGATTTT ACATGCTTTT AATTCAAGGT
    TTGCAGATAA ATGATTATGT GTGCCATCCT GTCTTGGCCA GCGTTATTCT AAACTCCCCT
    CCTTTGTTCT GCTGTGACTT GAAAGGGATT GATGTTGTGG TTCCTTACTT TATTTCAGCT
    CTTGAAACCA TTTTGCCTGA CAGAGAACTC TCAAAATTCA AAAGCTATGT AAATCCAACA
    GAATTGAGAA GATCTTCCAT TAATATCCTC CTTTCTTTAT TGCCCCTCCC TCATCATTTT
    GGCACAGTCA GATCTGAGGT GGTCCTGGAA GGGAAGTTTA GTAATGATGA CAGCTCTTCA
    TATGATAAAC CAATAACTTT TTTGTCCCTG AAGTTGAGAC TTGTGAATAT ACTAATAGGT
    GCCTTGCAAA CGGAAACGGA CCCCAACAAC ACTCAAATGA TACTAGGGGC AATGTTAAAT
    ATTGTTCAAG ATTCAGCACT TTTAGAAGCC ATTGGTTGCC AAATGGAGAT GGGTGGTGGA
    GAAAATAACC TGAAGAGTCA TAGTCGTACT AATAGTGGTA TTAGTTCAGC AAGTGGTGGA
    AGCACAGAAC CCACAACTCC TGATAGTGAA AGACCTGCTC AAGCTCTCCT AAGGGATTAT
    GGATCGACAG CTCTTAATAC AGATTCAGCT GCTGGGCTCC TGATTCGCAG CATTCATCTT
    GTCACCCAAA GACTCAACTC TCAGTGGCGG CAAGACATGA GCATATCACT GGCAGCGCTG
    GAGCTCCTCT CTGGCCTGGC AAAGGTGAAG GTGATGGTTG ACTCGGGAGA CCGGAAACGA
    GCCATCAGTT CTGTGTGCAG CTACATCGTG TACCAATGTA GTCGGCCAGC TCCTCTTCAC
    TCCCGCGATC TGCACTCCAT GATAGTAGCA GCTTTTCAGT GTCTCTGTGT CTGGCTGACA
    GAGCATCCTG ATATGCTTGA TGAAAAGGAC TGTCTTAAGG AAGTATTGGA GATTGTGGAA
    CTGGGTATTT CAGGAAGTAA GTCCAAGAAC AGTGAGCAAG AGGTCAAGTA CAAAGGAGAT
    AAGGAGCCAA ACCCTGCATC TATGAGGGTA AAGGATGCTG CTGAAGCCAC TCTAACATGT
    ATTATGCAGT TGCTCGGCGC GTTTCCTTCA CCCAGTGGTC CTGCCTCTCC TTGTAGTCTG
    GTGAATGAGA CCACTTTGAT TAAATACTCA AGGCTGCCAA CCATAAACAA GCATAGTTTC
    CGGTACTTTG TCTTGGATAA CAGTGTCATC CTGGCAATGC TGGAGCAACC TCTTGGAAAT
    GAACAGAATG ATTTTTTTCC CTCTGTCACT GTGCTGGTTC GAGGAATGTC TGGAAGACTT
    GCTTGGGCAC AACAACTTTG CCTTTTACCC AGAGGAGCAA AAGCAAATCA GAAGCTTTTT
    GTACCTGAAC CTCGTCCAAT TCCTAAAAAT GATGTTGGAT TTAAATATAC TGTCAAACAT
    CGACCATTTC CTGAAGAGGT GGATAAGATT CCTTTTGTGA AAGCAGATCT GAGCATTCCA
    GATTTGCATG AAATTGTCAC TGAAGAATTA GAAGAGAGAC ATGAAAAATT AAGGAATGGC
    ATGGCCCAGC AGATTGCTTA TGAAATACAC CTTGAACAAC AAAGTGAGGA AGAATTGCAG
    AAGAGAAGTT TTCCTGATCC AATTACGGAT TGTAAGCCTC CACCTCCTGC CCAAGAATTC
    CAAACAGCAC GCCTCTTCCT TTCACACTTT GGATTTTTGT CTTTAGAAGC ATTGAAGGAA
    CCTGCAAATA GTCGCCTACC TCCTCACCTT ATTGCACTTG ACTCCACGAT ACCTGGATTT
    TTTGATGACA TTGGATATCT GGATCTCTTG CCGTGTCGTC CCTTTGACAC AGTTTTTATT
    TTCTATATGA AACCAGGTCA GAAAACAAAC CAGGAGATTT TAAAGAATGT GGAATCTTCC
    AGAAATGTTC AGCCACATTT CCTAGAATTT TTGCTCTCCC TTGGCTGGTC AGTAGATGTG
    GGCAGACACC CAGGTTGGAC TGGGCATGTT TCTACCAGTT GGTCTATTAA CAGTTGTGAT
    GATGGTGAAG GACCTGAACA AGATGAAGTG ATTTCCTCTG AAGACATTGG GGCTAGCATT
    TTCAATGGAC AGAAGAAGGT GCTGTATTAT GCTGATGCCC TTACGGAAAT AGCTTTTGTA
    GTTCCTTCTC CTGTGGAGTC TTTAACTGAT TCATTAGAAA GTAACATCTC GGACCAAGAT
    AGTGATTCAA ATATGGATCT TATGCCTGGA ATTCTGAAAC AGCCATCCCT GACACTTGAG
    CTTTTCCCTA ATCACACAGA CAATCTTAAT TCCTCACAGC GGCTTAGTCC CAGTTCCAGA
    ATGAAGAAGC TGCCTCAGGG TCGCCCTGTG CCTCCCCTTG GACCTGAGAC AAGAGTTTCT
    GTAGTCTGGG TGGAACGCTA TGATGATATA GAGAACTTTC CCCTCTCAGA CCTGCTGACA
    GAGATCAGTA CTGGTGTGGA AACTACTGCA AACAGCAGCA CTTCTCTGAG ATCTACCACT
    CTTGAAAAAG AAGTTCCTGT CATCTTTATC CACCCTTTAA ACACTGGATT ATTCCGGATA
    AAAATTCAAG GAGCCACTGG AAAATTTAAC ATGGTCATCC CGCTCGTGGA TGGGATGATT
    GTCAGCAGAC GAGCACTTGG TTTTCTGGTG AGGCAGACTG TAATTAATAT TTGTAGAAGA
    AAGAGACTGG AGAGTGACTC CTACAGTCCA CCACATGTCC GCCGGAAACA GAAAATCACC
    GACATTGTCA ACAAGTACCG GAACAAGCAG TTGGAGCCAG AGTTTTATAC TTCACTTTTC
    CAGGAGGTTG GACTCAAGAA CTGCTCTTCT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005635106.1
    CDS152..4684
    Translation

    Target ORF information:

    RefSeq Version XM_005635049.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005635049.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATCCAGA ATGATCAGGG CCACACGAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGG CGAGAGGTGG CAAATGCAGT GGTCCATCCC
    CTTGGGCAGG CCTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAGTTTGTT AAAAACTGAC
    AAAGAAGTGA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGTGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTATTGCCG
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC TATATGTTCA AAGTATACTA
    AAACACCTGC AAAATCTTTT TGTACCAAGA CAGGAACAGG GCTCCAGTCA GATCCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTAGCCCGTG AGTCATCCAT CATGGCACGA
    GAAACGTGGG AAGTCTTACT ATTGTTTCTT CTGCAGATTA ATGACATACT TCTAGCCCCG
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTTTGGT TACTAGCTTG TACTCGGTGC TTTCCAACAC CTCCTTATTG GAAAACCGCC
    AAGGAGATGG TGGCAAACTG GAGGCACCAC CCGGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ACCTGTGCCC TCACTTCAAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTCCATTT
    AAAGTTCCTG ATGAAGATGC CAGTCTGATC CCTCCTGAAA TGGATAATGA GTGTGTAGCA
    CAGACATGGT TTCGCTTCTT ACATATGTTA AGTAATCCCG TGGATTTGAG TAACCCGGCC
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTAA ATGTGAGCGG AATGCCACAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTAGTGGA TGCATTCTTA GGCATTTCTA GACCCCGATC AGACAGTGCT
    CCTCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGCA CTGCTGTCAG TACCACCCCA
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC GAAAGTTCAG CACCAGACAT CCTCCACCTC TCCTCTGTCA
    AGTCCCAATC AGACTAGTTC TGAACCCCGG CCACTGCCTG CCCCTCGAAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCAT CTTTTATCCA AATTCTTCTT
    TCTTATAAAT CTTCCATTAC AACACAAGCT AGCATGGAGT TTCGACGGAA AGGGTCACAA
    ATGTCCACAG ACACCATGGT TTCCAATCCT ATGTTTGATG CAAGTGAATT TCCTGATAAC
    TATGAAGCAG GAAGAGCTGA GGCTTGTGGG ACACTGTGTA GGATTTTTTG TAGCAAGAAG
    ACTGGAGAAG AGATTCTGCC AGCTTATTTA TCCAGATTTT ACATGCTTTT AATTCAAGGT
    TTGCAGATAA ATGATTATGT GTGCCATCCT GTCTTGGCCA GCGTTATTCT AAACTCCCCT
    CCTTTGTTCT GCTGTGACTT GAAAGGGATT GATGTTGTGG TTCCTTACTT TATTTCAGCT
    CTTGAAACCA TTTTGCCTGA CAGAGAACTC TCAAAATTCA AAAGCTATGT AAATCCAACA
    GAATTGAGAA GATCTTCCAT TAATATCCTC CTTTCTTTAT TGCCCCTCCC TCATCATTTT
    GGCACAGTCA GATCTGAGGT GGTCCTGGAA GGGAAGTTTA GTAATGATGA CAGCTCTTCA
    TATGATAAAC CAATAACTTT TTTGTCCCTG AAGTTGAGAC TTGTGAATAT ACTAATAGGT
    GCCTTGCAAA CGGAAACGGA CCCCAACAAC ACTCAAATGA TACTAGGGGC AATGTTAAAT
    ATTGTTCAAG ATTCAGCACT TTTAGAAGCC ATTGGTTGCC AAATGGAGAT GGGTGGTGGA
    GAAAATAACC TGAAGAGTCA TAGTCGTACT AATAGTGGTA TTAGTTCAGC AAGTGGTGGA
    AGCACAGAAC CCACAACTCC TGATAGTGAA AGACCTGCTC AAGCTCTCCT AAGGGATTAT
    GGATCGACAG CTCTTAATAC AGATTCAGCT GCTGGGCTCC TGATTCGCAG CATTCATCTT
    GTCACCCAAA GACTCAACTC TCAGTGGCGG CAAGACATGA GCATATCACT GGCAGCGCTG
    GAGCTCCTCT CTGGCCTGGC AAAGGTGAAG GTGATGGTTG ACTCGGGAGA CCGGAAACGA
    GCCATCAGTT CTGTGTGCAG CTACATCGTG TACCAATGTA GTCGGCCAGC TCCTCTTCAC
    TCCCGCGATC TGCACTCCAT GATAGTAGCA GCTTTTCAGT GTCTCTGTGT CTGGCTGACA
    GAGCATCCTG ATATGCTTGA TGAAAAGGAC TGTCTTAAGG AAGTATTGGA GATTGTGGAA
    CTGGGTATTT CAGGAAGTAA GTCCAAGAAC AGTGAGCAAG AGGTCAAGTA CAAAGGAGAT
    AAGGAGCCAA ACCCTGCATC TATGAGGGTA AAGGATGCTG CTGAAGCCAC TCTAACATGT
    ATTATGCAGT TGCTCGGCGC GTTTCCTTCA CCCAGTGGTC CTGCCTCTCC TTGTAGTCTG
    GTGAATGAGA CCACTTTGAT TAAATACTCA AGGCTGCCAA CCATAAACAA GCATAGTTTC
    CGGTACTTTG TCTTGGATAA CAGTGTCATC CTGGCAATGC TGGAGCAACC TCTTGGAAAT
    GAACAGAATG ATTTTTTTCC CTCTGTCACT GTGCTGGTTC GAGGAATGTC TGGAAGACTT
    GCTTGGGCAC AACAACTTTG CCTTTTACCC AGAGGAGCAA AAGCAAATCA GAAGCTTTTT
    GTACCTGAAC CTCGTCCAAT TCCTAAAAAT GATGTTGGAT TTAAATATAC TGTCAAACAT
    CGACCATTTC CTGAAGAGGT GGATAAGATT CCTTTTGTGA AAGCAGATCT GAGCATTCCA
    GATTTGCATG AAATTGTCAC TGAAGAATTA GAAGAGAGAC ATGAAAAATT AAGGAATGGC
    ATGGCCCAGC AGATTGCTTA TGAAATACAC CTTGAACAAC AAAGTGAGGA AGAATTGCAG
    AAGAGAAGTT TTCCTGATCC AATTACGGAT TGTAAGCCTC CACCTCCTGC CCAAGAATTC
    CAAACAGCAC GCCTCTTCCT TTCACACTTT GGATTTTTGT CTTTAGAAGC ATTGAAGGAA
    CCTGCAAATA GTCGCCTACC TCCTCACCTT ATTGCACTTG ACTCCACGAT ACCTGGATTT
    TTTGATGACA TTGGATATCT GGATCTCTTG CCGTGTCGTC CCTTTGACAC AGTTTTTATT
    TTCTATATGA AACCAGGTCA GAAAACAAAC CAGGAGATTT TAAAGAATGT GGAATCTTCC
    AGAAATGTTC AGCCACATTT CCTAGAATTT TTGCTCTCCC TTGGCTGGTC AGTAGATGTG
    GGCAGACACC CAGGTTGGAC TGGGCATGTT TCTACCAGTT GGTCTATTAA CAGTTGTGAT
    GATGGTGAAG GACCTGAACA AGATGAAGTG ATTTCCTCTG AAGACATTGG GGCTAGCATT
    TTCAATGGAC AGAAGAAGGT GCTGTATTAT GCTGATGCCC TTACGGAAAT AGCTTTTGTA
    GTTCCTTCTC CTGTGGAGTC TTTAACTGAT TCATTAGAAA GTAACATCTC GGACCAAGAT
    AGTGATTCAA ATATGGATCT TATGCCTGGA ATTCTGAAAC AGCCATCCCT GACACTTGAG
    CTTTTCCCTA ATCACACAGA CAATCTTAAT TCCTCACAGC GGCTTAGTCC CAGTTCCAGA
    ATGAAGAAGC TGCCTCAGGG TCGCCCTGTG CCTCCCCTTG GACCTGAGAC AAGAGTTTCT
    GTAGTCTGGG TGGAACGCTA TGATGATATA GAGAACTTTC CCCTCTCAGA CCTGCTGACA
    GAGATCAGTA CTGGTGTGGA AACTACTGCA AACAGCAGCA CTTCTCTGAG ATCTACCACT
    CTTGAAAAAG AAGTTCCTGT CATCTTTATC CACCCTTTAA ACACTGGATT ATTCCGGATA
    AAAATTCAAG GAGCCACTGG AAAATTTAAC ATGGTCATCC CGCTCGTGGA TGGGATGATT
    GTCAGCAGAC GAGCACTTGG TTTTCTGGTG AGGCAGACTG TAATTAATAT TTGTAGAAGA
    AAGAGACTGG AGAGTGACTC CTACAGTCCA CCACATGTCC GCCGGAAACA GAAAATCACC
    GACATTGTCA ACAAGTACCG GAACAAGCAG TTGGAGCCAG AGTTTTATAC TTCACTTTTC
    CAGGAGGTTG GACTCAAGAA CTGCTCTTCT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe24888
    Clone ID Related Accession (Same CDS sequence) XM_022409441.1 , XM_005635050.3 , XM_005635049.2 , XM_005635048.2 , XM_005635050.2 , XM_005635049.3 , XM_005635048.3
    Accession Version XM_005635048.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4533bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ral GTPase-activating protein subunit beta isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005635048.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATCCAGA ATGATCAGGG CCACACGAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGG CGAGAGGTGG CAAATGCAGT GGTCCATCCC
    CTTGGGCAGG CCTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAGTTTGTT AAAAACTGAC
    AAAGAAGTGA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGTGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTATTGCCG
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC TATATGTTCA AAGTATACTA
    AAACACCTGC AAAATCTTTT TGTACCAAGA CAGGAACAGG GCTCCAGTCA GATCCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTAGCCCGTG AGTCATCCAT CATGGCACGA
    GAAACGTGGG AAGTCTTACT ATTGTTTCTT CTGCAGATTA ATGACATACT TCTAGCCCCG
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTTTGGT TACTAGCTTG TACTCGGTGC TTTCCAACAC CTCCTTATTG GAAAACCGCC
    AAGGAGATGG TGGCAAACTG GAGGCACCAC CCGGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ACCTGTGCCC TCACTTCAAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTCCATTT
    AAAGTTCCTG ATGAAGATGC CAGTCTGATC CCTCCTGAAA TGGATAATGA GTGTGTAGCA
    CAGACATGGT TTCGCTTCTT ACATATGTTA AGTAATCCCG TGGATTTGAG TAACCCGGCC
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTAA ATGTGAGCGG AATGCCACAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTAGTGGA TGCATTCTTA GGCATTTCTA GACCCCGATC AGACAGTGCT
    CCTCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGCA CTGCTGTCAG TACCACCCCA
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC GAAAGTTCAG CACCAGACAT CCTCCACCTC TCCTCTGTCA
    AGTCCCAATC AGACTAGTTC TGAACCCCGG CCACTGCCTG CCCCTCGAAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCAT CTTTTATCCA AATTCTTCTT
    TCTTATAAAT CTTCCATTAC AACACAAGCT AGCATGGAGT TTCGACGGAA AGGGTCACAA
    ATGTCCACAG ACACCATGGT TTCCAATCCT ATGTTTGATG CAAGTGAATT TCCTGATAAC
    TATGAAGCAG GAAGAGCTGA GGCTTGTGGG ACACTGTGTA GGATTTTTTG TAGCAAGAAG
    ACTGGAGAAG AGATTCTGCC AGCTTATTTA TCCAGATTTT ACATGCTTTT AATTCAAGGT
    TTGCAGATAA ATGATTATGT GTGCCATCCT GTCTTGGCCA GCGTTATTCT AAACTCCCCT
    CCTTTGTTCT GCTGTGACTT GAAAGGGATT GATGTTGTGG TTCCTTACTT TATTTCAGCT
    CTTGAAACCA TTTTGCCTGA CAGAGAACTC TCAAAATTCA AAAGCTATGT AAATCCAACA
    GAATTGAGAA GATCTTCCAT TAATATCCTC CTTTCTTTAT TGCCCCTCCC TCATCATTTT
    GGCACAGTCA GATCTGAGGT GGTCCTGGAA GGGAAGTTTA GTAATGATGA CAGCTCTTCA
    TATGATAAAC CAATAACTTT TTTGTCCCTG AAGTTGAGAC TTGTGAATAT ACTAATAGGT
    GCCTTGCAAA CGGAAACGGA CCCCAACAAC ACTCAAATGA TACTAGGGGC AATGTTAAAT
    ATTGTTCAAG ATTCAGCACT TTTAGAAGCC ATTGGTTGCC AAATGGAGAT GGGTGGTGGA
    GAAAATAACC TGAAGAGTCA TAGTCGTACT AATAGTGGTA TTAGTTCAGC AAGTGGTGGA
    AGCACAGAAC CCACAACTCC TGATAGTGAA AGACCTGCTC AAGCTCTCCT AAGGGATTAT
    GGATCGACAG CTCTTAATAC AGATTCAGCT GCTGGGCTCC TGATTCGCAG CATTCATCTT
    GTCACCCAAA GACTCAACTC TCAGTGGCGG CAAGACATGA GCATATCACT GGCAGCGCTG
    GAGCTCCTCT CTGGCCTGGC AAAGGTGAAG GTGATGGTTG ACTCGGGAGA CCGGAAACGA
    GCCATCAGTT CTGTGTGCAG CTACATCGTG TACCAATGTA GTCGGCCAGC TCCTCTTCAC
    TCCCGCGATC TGCACTCCAT GATAGTAGCA GCTTTTCAGT GTCTCTGTGT CTGGCTGACA
    GAGCATCCTG ATATGCTTGA TGAAAAGGAC TGTCTTAAGG AAGTATTGGA GATTGTGGAA
    CTGGGTATTT CAGGAAGTAA GTCCAAGAAC AGTGAGCAAG AGGTCAAGTA CAAAGGAGAT
    AAGGAGCCAA ACCCTGCATC TATGAGGGTA AAGGATGCTG CTGAAGCCAC TCTAACATGT
    ATTATGCAGT TGCTCGGCGC GTTTCCTTCA CCCAGTGGTC CTGCCTCTCC TTGTAGTCTG
    GTGAATGAGA CCACTTTGAT TAAATACTCA AGGCTGCCAA CCATAAACAA GCATAGTTTC
    CGGTACTTTG TCTTGGATAA CAGTGTCATC CTGGCAATGC TGGAGCAACC TCTTGGAAAT
    GAACAGAATG ATTTTTTTCC CTCTGTCACT GTGCTGGTTC GAGGAATGTC TGGAAGACTT
    GCTTGGGCAC AACAACTTTG CCTTTTACCC AGAGGAGCAA AAGCAAATCA GAAGCTTTTT
    GTACCTGAAC CTCGTCCAAT TCCTAAAAAT GATGTTGGAT TTAAATATAC TGTCAAACAT
    CGACCATTTC CTGAAGAGGT GGATAAGATT CCTTTTGTGA AAGCAGATCT GAGCATTCCA
    GATTTGCATG AAATTGTCAC TGAAGAATTA GAAGAGAGAC ATGAAAAATT AAGGAATGGC
    ATGGCCCAGC AGATTGCTTA TGAAATACAC CTTGAACAAC AAAGTGAGGA AGAATTGCAG
    AAGAGAAGTT TTCCTGATCC AATTACGGAT TGTAAGCCTC CACCTCCTGC CCAAGAATTC
    CAAACAGCAC GCCTCTTCCT TTCACACTTT GGATTTTTGT CTTTAGAAGC ATTGAAGGAA
    CCTGCAAATA GTCGCCTACC TCCTCACCTT ATTGCACTTG ACTCCACGAT ACCTGGATTT
    TTTGATGACA TTGGATATCT GGATCTCTTG CCGTGTCGTC CCTTTGACAC AGTTTTTATT
    TTCTATATGA AACCAGGTCA GAAAACAAAC CAGGAGATTT TAAAGAATGT GGAATCTTCC
    AGAAATGTTC AGCCACATTT CCTAGAATTT TTGCTCTCCC TTGGCTGGTC AGTAGATGTG
    GGCAGACACC CAGGTTGGAC TGGGCATGTT TCTACCAGTT GGTCTATTAA CAGTTGTGAT
    GATGGTGAAG GACCTGAACA AGATGAAGTG ATTTCCTCTG AAGACATTGG GGCTAGCATT
    TTCAATGGAC AGAAGAAGGT GCTGTATTAT GCTGATGCCC TTACGGAAAT AGCTTTTGTA
    GTTCCTTCTC CTGTGGAGTC TTTAACTGAT TCATTAGAAA GTAACATCTC GGACCAAGAT
    AGTGATTCAA ATATGGATCT TATGCCTGGA ATTCTGAAAC AGCCATCCCT GACACTTGAG
    CTTTTCCCTA ATCACACAGA CAATCTTAAT TCCTCACAGC GGCTTAGTCC CAGTTCCAGA
    ATGAAGAAGC TGCCTCAGGG TCGCCCTGTG CCTCCCCTTG GACCTGAGAC AAGAGTTTCT
    GTAGTCTGGG TGGAACGCTA TGATGATATA GAGAACTTTC CCCTCTCAGA CCTGCTGACA
    GAGATCAGTA CTGGTGTGGA AACTACTGCA AACAGCAGCA CTTCTCTGAG ATCTACCACT
    CTTGAAAAAG AAGTTCCTGT CATCTTTATC CACCCTTTAA ACACTGGATT ATTCCGGATA
    AAAATTCAAG GAGCCACTGG AAAATTTAAC ATGGTCATCC CGCTCGTGGA TGGGATGATT
    GTCAGCAGAC GAGCACTTGG TTTTCTGGTG AGGCAGACTG TAATTAATAT TTGTAGAAGA
    AAGAGACTGG AGAGTGACTC CTACAGTCCA CCACATGTCC GCCGGAAACA GAAAATCACC
    GACATTGTCA ACAAGTACCG GAACAAGCAG TTGGAGCCAG AGTTTTATAC TTCACTTTTC
    CAGGAGGTTG GACTCAAGAA CTGCTCTTCT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005635105.1
    CDS278..4810
    Translation

    Target ORF information:

    RefSeq Version XM_005635048.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005635048.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATCCAGA ATGATCAGGG CCACACGAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGG CGAGAGGTGG CAAATGCAGT GGTCCATCCC
    CTTGGGCAGG CCTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAGTTTGTT AAAAACTGAC
    AAAGAAGTGA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGTGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTATTGCCG
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC TATATGTTCA AAGTATACTA
    AAACACCTGC AAAATCTTTT TGTACCAAGA CAGGAACAGG GCTCCAGTCA GATCCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTAGCCCGTG AGTCATCCAT CATGGCACGA
    GAAACGTGGG AAGTCTTACT ATTGTTTCTT CTGCAGATTA ATGACATACT TCTAGCCCCG
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTTTGGT TACTAGCTTG TACTCGGTGC TTTCCAACAC CTCCTTATTG GAAAACCGCC
    AAGGAGATGG TGGCAAACTG GAGGCACCAC CCGGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ACCTGTGCCC TCACTTCAAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTCCATTT
    AAAGTTCCTG ATGAAGATGC CAGTCTGATC CCTCCTGAAA TGGATAATGA GTGTGTAGCA
    CAGACATGGT TTCGCTTCTT ACATATGTTA AGTAATCCCG TGGATTTGAG TAACCCGGCC
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTAA ATGTGAGCGG AATGCCACAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTAGTGGA TGCATTCTTA GGCATTTCTA GACCCCGATC AGACAGTGCT
    CCTCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGCA CTGCTGTCAG TACCACCCCA
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC GAAAGTTCAG CACCAGACAT CCTCCACCTC TCCTCTGTCA
    AGTCCCAATC AGACTAGTTC TGAACCCCGG CCACTGCCTG CCCCTCGAAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCAT CTTTTATCCA AATTCTTCTT
    TCTTATAAAT CTTCCATTAC AACACAAGCT AGCATGGAGT TTCGACGGAA AGGGTCACAA
    ATGTCCACAG ACACCATGGT TTCCAATCCT ATGTTTGATG CAAGTGAATT TCCTGATAAC
    TATGAAGCAG GAAGAGCTGA GGCTTGTGGG ACACTGTGTA GGATTTTTTG TAGCAAGAAG
    ACTGGAGAAG AGATTCTGCC AGCTTATTTA TCCAGATTTT ACATGCTTTT AATTCAAGGT
    TTGCAGATAA ATGATTATGT GTGCCATCCT GTCTTGGCCA GCGTTATTCT AAACTCCCCT
    CCTTTGTTCT GCTGTGACTT GAAAGGGATT GATGTTGTGG TTCCTTACTT TATTTCAGCT
    CTTGAAACCA TTTTGCCTGA CAGAGAACTC TCAAAATTCA AAAGCTATGT AAATCCAACA
    GAATTGAGAA GATCTTCCAT TAATATCCTC CTTTCTTTAT TGCCCCTCCC TCATCATTTT
    GGCACAGTCA GATCTGAGGT GGTCCTGGAA GGGAAGTTTA GTAATGATGA CAGCTCTTCA
    TATGATAAAC CAATAACTTT TTTGTCCCTG AAGTTGAGAC TTGTGAATAT ACTAATAGGT
    GCCTTGCAAA CGGAAACGGA CCCCAACAAC ACTCAAATGA TACTAGGGGC AATGTTAAAT
    ATTGTTCAAG ATTCAGCACT TTTAGAAGCC ATTGGTTGCC AAATGGAGAT GGGTGGTGGA
    GAAAATAACC TGAAGAGTCA TAGTCGTACT AATAGTGGTA TTAGTTCAGC AAGTGGTGGA
    AGCACAGAAC CCACAACTCC TGATAGTGAA AGACCTGCTC AAGCTCTCCT AAGGGATTAT
    GGATCGACAG CTCTTAATAC AGATTCAGCT GCTGGGCTCC TGATTCGCAG CATTCATCTT
    GTCACCCAAA GACTCAACTC TCAGTGGCGG CAAGACATGA GCATATCACT GGCAGCGCTG
    GAGCTCCTCT CTGGCCTGGC AAAGGTGAAG GTGATGGTTG ACTCGGGAGA CCGGAAACGA
    GCCATCAGTT CTGTGTGCAG CTACATCGTG TACCAATGTA GTCGGCCAGC TCCTCTTCAC
    TCCCGCGATC TGCACTCCAT GATAGTAGCA GCTTTTCAGT GTCTCTGTGT CTGGCTGACA
    GAGCATCCTG ATATGCTTGA TGAAAAGGAC TGTCTTAAGG AAGTATTGGA GATTGTGGAA
    CTGGGTATTT CAGGAAGTAA GTCCAAGAAC AGTGAGCAAG AGGTCAAGTA CAAAGGAGAT
    AAGGAGCCAA ACCCTGCATC TATGAGGGTA AAGGATGCTG CTGAAGCCAC TCTAACATGT
    ATTATGCAGT TGCTCGGCGC GTTTCCTTCA CCCAGTGGTC CTGCCTCTCC TTGTAGTCTG
    GTGAATGAGA CCACTTTGAT TAAATACTCA AGGCTGCCAA CCATAAACAA GCATAGTTTC
    CGGTACTTTG TCTTGGATAA CAGTGTCATC CTGGCAATGC TGGAGCAACC TCTTGGAAAT
    GAACAGAATG ATTTTTTTCC CTCTGTCACT GTGCTGGTTC GAGGAATGTC TGGAAGACTT
    GCTTGGGCAC AACAACTTTG CCTTTTACCC AGAGGAGCAA AAGCAAATCA GAAGCTTTTT
    GTACCTGAAC CTCGTCCAAT TCCTAAAAAT GATGTTGGAT TTAAATATAC TGTCAAACAT
    CGACCATTTC CTGAAGAGGT GGATAAGATT CCTTTTGTGA AAGCAGATCT GAGCATTCCA
    GATTTGCATG AAATTGTCAC TGAAGAATTA GAAGAGAGAC ATGAAAAATT AAGGAATGGC
    ATGGCCCAGC AGATTGCTTA TGAAATACAC CTTGAACAAC AAAGTGAGGA AGAATTGCAG
    AAGAGAAGTT TTCCTGATCC AATTACGGAT TGTAAGCCTC CACCTCCTGC CCAAGAATTC
    CAAACAGCAC GCCTCTTCCT TTCACACTTT GGATTTTTGT CTTTAGAAGC ATTGAAGGAA
    CCTGCAAATA GTCGCCTACC TCCTCACCTT ATTGCACTTG ACTCCACGAT ACCTGGATTT
    TTTGATGACA TTGGATATCT GGATCTCTTG CCGTGTCGTC CCTTTGACAC AGTTTTTATT
    TTCTATATGA AACCAGGTCA GAAAACAAAC CAGGAGATTT TAAAGAATGT GGAATCTTCC
    AGAAATGTTC AGCCACATTT CCTAGAATTT TTGCTCTCCC TTGGCTGGTC AGTAGATGTG
    GGCAGACACC CAGGTTGGAC TGGGCATGTT TCTACCAGTT GGTCTATTAA CAGTTGTGAT
    GATGGTGAAG GACCTGAACA AGATGAAGTG ATTTCCTCTG AAGACATTGG GGCTAGCATT
    TTCAATGGAC AGAAGAAGGT GCTGTATTAT GCTGATGCCC TTACGGAAAT AGCTTTTGTA
    GTTCCTTCTC CTGTGGAGTC TTTAACTGAT TCATTAGAAA GTAACATCTC GGACCAAGAT
    AGTGATTCAA ATATGGATCT TATGCCTGGA ATTCTGAAAC AGCCATCCCT GACACTTGAG
    CTTTTCCCTA ATCACACAGA CAATCTTAAT TCCTCACAGC GGCTTAGTCC CAGTTCCAGA
    ATGAAGAAGC TGCCTCAGGG TCGCCCTGTG CCTCCCCTTG GACCTGAGAC AAGAGTTTCT
    GTAGTCTGGG TGGAACGCTA TGATGATATA GAGAACTTTC CCCTCTCAGA CCTGCTGACA
    GAGATCAGTA CTGGTGTGGA AACTACTGCA AACAGCAGCA CTTCTCTGAG ATCTACCACT
    CTTGAAAAAG AAGTTCCTGT CATCTTTATC CACCCTTTAA ACACTGGATT ATTCCGGATA
    AAAATTCAAG GAGCCACTGG AAAATTTAAC ATGGTCATCC CGCTCGTGGA TGGGATGATT
    GTCAGCAGAC GAGCACTTGG TTTTCTGGTG AGGCAGACTG TAATTAATAT TTGTAGAAGA
    AAGAGACTGG AGAGTGACTC CTACAGTCCA CCACATGTCC GCCGGAAACA GAAAATCACC
    GACATTGTCA ACAAGTACCG GAACAAGCAG TTGGAGCCAG AGTTTTATAC TTCACTTTTC
    CAGGAGGTTG GACTCAAGAA CTGCTCTTCT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe24889
    Clone ID Related Accession (Same CDS sequence) XM_022409442.1 , XM_005635051.2
    Accession Version XM_022409442.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4524bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ral GTPase-activating protein subunit beta isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATCCAGA ATGATCAGGG CCACACGAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGG CGAGAGGTGG CAAATGCAGT GGTCCATCCC
    CTTGGGCAGG CCTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAGTTTGTT AAAAACTGAC
    AAAGAAGTGA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGTGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTATTGCCG
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC TATATGTTCA AAGTATACTA
    AAACACCTGC AAAATCTTTT TGTACCAAGA CAGGAACAGG GCTCCAGTCA GATCCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTAGCCCGTG AGTCATCCAT CATGGCACGA
    GAAACGTGGG AAGTCTTACT ATTGTTTCTT CTGCAGATTA ATGACATACT TCTAGCCCCG
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTTTGGT TACTAGCTTG TACTCGGTGC TTTCCAACAC CTCCTTATTG GAAAACCGCC
    AAGGAGATGG TGGCAAACTG GAGGCACCAC CCGGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ACCTGTGCCC TCACTTCAAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTCCATTT
    AAAGTTCCTG ATGAAGATGC CAGTCTGATC CCTCCTGAAA TGGATAATGA GTGTGTAGCA
    CAGACATGGT TTCGCTTCTT ACATATGTTA AGTAATCCCG TGGATTTGAG TAACCCGGCC
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTAA ATGTGAGCGG AATGCCACAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTAGTGGA TGCATTCTTA GGCATTTCTA GACCCCGATC AGACAGTGCT
    CCTCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGCA CTGCTGTCAG TACCACCCCA
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC GAAAGTTCAG CACCAGACAT CCTCCACCTC TCCTCTGTCA
    AGTCCCAATC AGACTAGTTC TGAACCCCGG CCACTGCCTG CCCCTCGAAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCAT CTTTTATCCA AATTCTTCTT
    TCTTATAAAT CTTCCATTAC AACACAAGCT AGCATGGAGT TTCGACGGAA AGGGTCACAA
    ATGTCCACAG ACACCATGGT TTCCAATCCT ATGTTTGATG CAAGTGAATT TCCTGATAAC
    TATGAAGCAG GAAGAGCTGA GGCTTGTGGG ACACTGTGTA GGATTTTTTG TAGCAAGAAG
    ACTGGAGAAG AGATTCTGCC AGCTTATTTA TCCAGATTTT ACATGCTTTT AATTCAAGGT
    TTGCAGATAA ATGATTATGT GTGCCATCCT GTCTTGGCCA GCGTTATTCT AAACTCCCCT
    CCTTTGTTCT GCTGTGACTT GAAAGGGATT GATGTTGTGG TTCCTTACTT TATTTCAGCT
    CTTGAAACCA TTTTGCCTGA CAGAGAACTC TCAAAATTCA AAAGCTATGT AAATCCAACA
    GAATTGAGAA GATCTTCCAT TAATATCCTC CTTTCTTTAT TGCCCCTCCC TCATCATTTT
    GGCACAGTCA GATCTGAGGT GGTCCTGGAA GGGAAGTTTA GTAATGATGA CAGCTCTTCA
    TATGATAAAC CAATAACTTT TTTGTCCCTG AAGTTGAGAC TTGTGAATAT ACTAATAGGT
    GCCTTGCAAA CGGAAACGGA CCCCAACAAC ACTCAAATGA TACTAGGGGC AATGTTAAAT
    ATTGTTCAAG ATTCAGCACT TTTAGAAGCC ATTGGTTGCC AAATGGAGAT GGGTGGTGGA
    GAAAATAACC TGAAGAGTCA TAGTCGTACT AATAGTGGTA TTAGTTCAGC AAGTGGTGGA
    AGCACAGAAC CCACAACTCC TGATAGTGAA AGACCTGCTC AAGCTCTCCT AAGGGATTAT
    GCTCTTAATA CAGATTCAGC TGCTGGGCTC CTGATTCGCA GCATTCATCT TGTCACCCAA
    AGACTCAACT CTCAGTGGCG GCAAGACATG AGCATATCAC TGGCAGCGCT GGAGCTCCTC
    TCTGGCCTGG CAAAGGTGAA GGTGATGGTT GACTCGGGAG ACCGGAAACG AGCCATCAGT
    TCTGTGTGCA GCTACATCGT GTACCAATGT AGTCGGCCAG CTCCTCTTCA CTCCCGCGAT
    CTGCACTCCA TGATAGTAGC AGCTTTTCAG TGTCTCTGTG TCTGGCTGAC AGAGCATCCT
    GATATGCTTG ATGAAAAGGA CTGTCTTAAG GAAGTATTGG AGATTGTGGA ACTGGGTATT
    TCAGGAAGTA AGTCCAAGAA CAGTGAGCAA GAGGTCAAGT ACAAAGGAGA TAAGGAGCCA
    AACCCTGCAT CTATGAGGGT AAAGGATGCT GCTGAAGCCA CTCTAACATG TATTATGCAG
    TTGCTCGGCG CGTTTCCTTC ACCCAGTGGT CCTGCCTCTC CTTGTAGTCT GGTGAATGAG
    ACCACTTTGA TTAAATACTC AAGGCTGCCA ACCATAAACA AGCATAGTTT CCGGTACTTT
    GTCTTGGATA ACAGTGTCAT CCTGGCAATG CTGGAGCAAC CTCTTGGAAA TGAACAGAAT
    GATTTTTTTC CCTCTGTCAC TGTGCTGGTT CGAGGAATGT CTGGAAGACT TGCTTGGGCA
    CAACAACTTT GCCTTTTACC CAGAGGAGCA AAAGCAAATC AGAAGCTTTT TGTACCTGAA
    CCTCGTCCAA TTCCTAAAAA TGATGTTGGA TTTAAATATA CTGTCAAACA TCGACCATTT
    CCTGAAGAGG TGGATAAGAT TCCTTTTGTG AAAGCAGATC TGAGCATTCC AGATTTGCAT
    GAAATTGTCA CTGAAGAATT AGAAGAGAGA CATGAAAAAT TAAGGAATGG CATGGCCCAG
    CAGATTGCTT ATGAAATACA CCTTGAACAA CAAAGTGAGG AAGAATTGCA GAAGAGAAGT
    TTTCCTGATC CAATTACGGA TTGTAAGCCT CCACCTCCTG CCCAAGAATT CCAAACAGCA
    CGCCTCTTCC TTTCACACTT TGGATTTTTG TCTTTAGAAG CATTGAAGGA ACCTGCAAAT
    AGTCGCCTAC CTCCTCACCT TATTGCACTT GACTCCACGA TACCTGGATT TTTTGATGAC
    ATTGGATATC TGGATCTCTT GCCGTGTCGT CCCTTTGACA CAGTTTTTAT TTTCTATATG
    AAACCAGGTC AGAAAACAAA CCAGGAGATT TTAAAGAATG TGGAATCTTC CAGAAATGTT
    CAGCCACATT TCCTAGAATT TTTGCTCTCC CTTGGCTGGT CAGTAGATGT GGGCAGACAC
    CCAGGTTGGA CTGGGCATGT TTCTACCAGT TGGTCTATTA ACAGTTGTGA TGATGGTGAA
    GGACCTGAAC AAGATGAAGT GATTTCCTCT GAAGACATTG GGGCTAGCAT TTTCAATGGA
    CAGAAGAAGG TGCTGTATTA TGCTGATGCC CTTACGGAAA TAGCTTTTGT AGTTCCTTCT
    CCTGTGGAGT CTTTAACTGA TTCATTAGAA AGTAACATCT CGGACCAAGA TAGTGATTCA
    AATATGGATC TTATGCCTGG AATTCTGAAA CAGCCATCCC TGACACTTGA GCTTTTCCCT
    AATCACACAG ACAATCTTAA TTCCTCACAG CGGCTTAGTC CCAGTTCCAG AATGAAGAAG
    CTGCCTCAGG GTCGCCCTGT GCCTCCCCTT GGACCTGAGA CAAGAGTTTC TGTAGTCTGG
    GTGGAACGCT ATGATGATAT AGAGAACTTT CCCCTCTCAG ACCTGCTGAC AGAGATCAGT
    ACTGGTGTGG AAACTACTGC AAACAGCAGC ACTTCTCTGA GATCTACCAC TCTTGAAAAA
    GAAGTTCCTG TCATCTTTAT CCACCCTTTA AACACTGGAT TATTCCGGAT AAAAATTCAA
    GGAGCCACTG GAAAATTTAA CATGGTCATC CCGCTCGTGG ATGGGATGAT TGTCAGCAGA
    CGAGCACTTG GTTTTCTGGT GAGGCAGACT GTAATTAATA TTTGTAGAAG AAAGAGACTG
    GAGAGTGACT CCTACAGTCC ACCACATGTC CGCCGGAAAC AGAAAATCAC CGACATTGTC
    AACAAGTACC GGAACAAGCA GTTGGAGCCA GAGTTTTATA CTTCACTTTT CCAGGAGGTT
    GGACTCAAGA ACTGCTCTTC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022265150.1
    CDS369..4892
    Translation

    Target ORF information:

    RefSeq Version XM_022409442.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022409442.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATCCAGA ATGATCAGGG CCACACGAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGG CGAGAGGTGG CAAATGCAGT GGTCCATCCC
    CTTGGGCAGG CCTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAGTTTGTT AAAAACTGAC
    AAAGAAGTGA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGTGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTATTGCCG
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC TATATGTTCA AAGTATACTA
    AAACACCTGC AAAATCTTTT TGTACCAAGA CAGGAACAGG GCTCCAGTCA GATCCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTAGCCCGTG AGTCATCCAT CATGGCACGA
    GAAACGTGGG AAGTCTTACT ATTGTTTCTT CTGCAGATTA ATGACATACT TCTAGCCCCG
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTTTGGT TACTAGCTTG TACTCGGTGC TTTCCAACAC CTCCTTATTG GAAAACCGCC
    AAGGAGATGG TGGCAAACTG GAGGCACCAC CCGGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ACCTGTGCCC TCACTTCAAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTCCATTT
    AAAGTTCCTG ATGAAGATGC CAGTCTGATC CCTCCTGAAA TGGATAATGA GTGTGTAGCA
    CAGACATGGT TTCGCTTCTT ACATATGTTA AGTAATCCCG TGGATTTGAG TAACCCGGCC
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTAA ATGTGAGCGG AATGCCACAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTAGTGGA TGCATTCTTA GGCATTTCTA GACCCCGATC AGACAGTGCT
    CCTCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGCA CTGCTGTCAG TACCACCCCA
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC GAAAGTTCAG CACCAGACAT CCTCCACCTC TCCTCTGTCA
    AGTCCCAATC AGACTAGTTC TGAACCCCGG CCACTGCCTG CCCCTCGAAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCAT CTTTTATCCA AATTCTTCTT
    TCTTATAAAT CTTCCATTAC AACACAAGCT AGCATGGAGT TTCGACGGAA AGGGTCACAA
    ATGTCCACAG ACACCATGGT TTCCAATCCT ATGTTTGATG CAAGTGAATT TCCTGATAAC
    TATGAAGCAG GAAGAGCTGA GGCTTGTGGG ACACTGTGTA GGATTTTTTG TAGCAAGAAG
    ACTGGAGAAG AGATTCTGCC AGCTTATTTA TCCAGATTTT ACATGCTTTT AATTCAAGGT
    TTGCAGATAA ATGATTATGT GTGCCATCCT GTCTTGGCCA GCGTTATTCT AAACTCCCCT
    CCTTTGTTCT GCTGTGACTT GAAAGGGATT GATGTTGTGG TTCCTTACTT TATTTCAGCT
    CTTGAAACCA TTTTGCCTGA CAGAGAACTC TCAAAATTCA AAAGCTATGT AAATCCAACA
    GAATTGAGAA GATCTTCCAT TAATATCCTC CTTTCTTTAT TGCCCCTCCC TCATCATTTT
    GGCACAGTCA GATCTGAGGT GGTCCTGGAA GGGAAGTTTA GTAATGATGA CAGCTCTTCA
    TATGATAAAC CAATAACTTT TTTGTCCCTG AAGTTGAGAC TTGTGAATAT ACTAATAGGT
    GCCTTGCAAA CGGAAACGGA CCCCAACAAC ACTCAAATGA TACTAGGGGC AATGTTAAAT
    ATTGTTCAAG ATTCAGCACT TTTAGAAGCC ATTGGTTGCC AAATGGAGAT GGGTGGTGGA
    GAAAATAACC TGAAGAGTCA TAGTCGTACT AATAGTGGTA TTAGTTCAGC AAGTGGTGGA
    AGCACAGAAC CCACAACTCC TGATAGTGAA AGACCTGCTC AAGCTCTCCT AAGGGATTAT
    GCTCTTAATA CAGATTCAGC TGCTGGGCTC CTGATTCGCA GCATTCATCT TGTCACCCAA
    AGACTCAACT CTCAGTGGCG GCAAGACATG AGCATATCAC TGGCAGCGCT GGAGCTCCTC
    TCTGGCCTGG CAAAGGTGAA GGTGATGGTT GACTCGGGAG ACCGGAAACG AGCCATCAGT
    TCTGTGTGCA GCTACATCGT GTACCAATGT AGTCGGCCAG CTCCTCTTCA CTCCCGCGAT
    CTGCACTCCA TGATAGTAGC AGCTTTTCAG TGTCTCTGTG TCTGGCTGAC AGAGCATCCT
    GATATGCTTG ATGAAAAGGA CTGTCTTAAG GAAGTATTGG AGATTGTGGA ACTGGGTATT
    TCAGGAAGTA AGTCCAAGAA CAGTGAGCAA GAGGTCAAGT ACAAAGGAGA TAAGGAGCCA
    AACCCTGCAT CTATGAGGGT AAAGGATGCT GCTGAAGCCA CTCTAACATG TATTATGCAG
    TTGCTCGGCG CGTTTCCTTC ACCCAGTGGT CCTGCCTCTC CTTGTAGTCT GGTGAATGAG
    ACCACTTTGA TTAAATACTC AAGGCTGCCA ACCATAAACA AGCATAGTTT CCGGTACTTT
    GTCTTGGATA ACAGTGTCAT CCTGGCAATG CTGGAGCAAC CTCTTGGAAA TGAACAGAAT
    GATTTTTTTC CCTCTGTCAC TGTGCTGGTT CGAGGAATGT CTGGAAGACT TGCTTGGGCA
    CAACAACTTT GCCTTTTACC CAGAGGAGCA AAAGCAAATC AGAAGCTTTT TGTACCTGAA
    CCTCGTCCAA TTCCTAAAAA TGATGTTGGA TTTAAATATA CTGTCAAACA TCGACCATTT
    CCTGAAGAGG TGGATAAGAT TCCTTTTGTG AAAGCAGATC TGAGCATTCC AGATTTGCAT
    GAAATTGTCA CTGAAGAATT AGAAGAGAGA CATGAAAAAT TAAGGAATGG CATGGCCCAG
    CAGATTGCTT ATGAAATACA CCTTGAACAA CAAAGTGAGG AAGAATTGCA GAAGAGAAGT
    TTTCCTGATC CAATTACGGA TTGTAAGCCT CCACCTCCTG CCCAAGAATT CCAAACAGCA
    CGCCTCTTCC TTTCACACTT TGGATTTTTG TCTTTAGAAG CATTGAAGGA ACCTGCAAAT
    AGTCGCCTAC CTCCTCACCT TATTGCACTT GACTCCACGA TACCTGGATT TTTTGATGAC
    ATTGGATATC TGGATCTCTT GCCGTGTCGT CCCTTTGACA CAGTTTTTAT TTTCTATATG
    AAACCAGGTC AGAAAACAAA CCAGGAGATT TTAAAGAATG TGGAATCTTC CAGAAATGTT
    CAGCCACATT TCCTAGAATT TTTGCTCTCC CTTGGCTGGT CAGTAGATGT GGGCAGACAC
    CCAGGTTGGA CTGGGCATGT TTCTACCAGT TGGTCTATTA ACAGTTGTGA TGATGGTGAA
    GGACCTGAAC AAGATGAAGT GATTTCCTCT GAAGACATTG GGGCTAGCAT TTTCAATGGA
    CAGAAGAAGG TGCTGTATTA TGCTGATGCC CTTACGGAAA TAGCTTTTGT AGTTCCTTCT
    CCTGTGGAGT CTTTAACTGA TTCATTAGAA AGTAACATCT CGGACCAAGA TAGTGATTCA
    AATATGGATC TTATGCCTGG AATTCTGAAA CAGCCATCCC TGACACTTGA GCTTTTCCCT
    AATCACACAG ACAATCTTAA TTCCTCACAG CGGCTTAGTC CCAGTTCCAG AATGAAGAAG
    CTGCCTCAGG GTCGCCCTGT GCCTCCCCTT GGACCTGAGA CAAGAGTTTC TGTAGTCTGG
    GTGGAACGCT ATGATGATAT AGAGAACTTT CCCCTCTCAG ACCTGCTGAC AGAGATCAGT
    ACTGGTGTGG AAACTACTGC AAACAGCAGC ACTTCTCTGA GATCTACCAC TCTTGAAAAA
    GAAGTTCCTG TCATCTTTAT CCACCCTTTA AACACTGGAT TATTCCGGAT AAAAATTCAA
    GGAGCCACTG GAAAATTTAA CATGGTCATC CCGCTCGTGG ATGGGATGAT TGTCAGCAGA
    CGAGCACTTG GTTTTCTGGT GAGGCAGACT GTAATTAATA TTTGTAGAAG AAAGAGACTG
    GAGAGTGACT CCTACAGTCC ACCACATGTC CGCCGGAAAC AGAAAATCAC CGACATTGTC
    AACAAGTACC GGAACAAGCA GTTGGAGCCA GAGTTTTATA CTTCACTTTT CCAGGAGGTT
    GGACTCAAGA ACTGCTCTTC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe24889
    Clone ID Related Accession (Same CDS sequence) XM_022409442.1 , XM_005635051.2
    Accession Version XM_005635051.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4524bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ral GTPase-activating protein subunit beta isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726101.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005635051.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATCCAGA ATGATCAGGG CCACACGAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGG CGAGAGGTGG CAAATGCAGT GGTCCATCCC
    CTTGGGCAGG CCTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAGTTTGTT AAAAACTGAC
    AAAGAAGTGA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGTGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTATTGCCG
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC TATATGTTCA AAGTATACTA
    AAACACCTGC AAAATCTTTT TGTACCAAGA CAGGAACAGG GCTCCAGTCA GATCCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTAGCCCGTG AGTCATCCAT CATGGCACGA
    GAAACGTGGG AAGTCTTACT ATTGTTTCTT CTGCAGATTA ATGACATACT TCTAGCCCCG
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTTTGGT TACTAGCTTG TACTCGGTGC TTTCCAACAC CTCCTTATTG GAAAACCGCC
    AAGGAGATGG TGGCAAACTG GAGGCACCAC CCGGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ACCTGTGCCC TCACTTCAAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTCCATTT
    AAAGTTCCTG ATGAAGATGC CAGTCTGATC CCTCCTGAAA TGGATAATGA GTGTGTAGCA
    CAGACATGGT TTCGCTTCTT ACATATGTTA AGTAATCCCG TGGATTTGAG TAACCCGGCC
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTAA ATGTGAGCGG AATGCCACAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTAGTGGA TGCATTCTTA GGCATTTCTA GACCCCGATC AGACAGTGCT
    CCTCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGCA CTGCTGTCAG TACCACCCCA
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC GAAAGTTCAG CACCAGACAT CCTCCACCTC TCCTCTGTCA
    AGTCCCAATC AGACTAGTTC TGAACCCCGG CCACTGCCTG CCCCTCGAAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCAT CTTTTATCCA AATTCTTCTT
    TCTTATAAAT CTTCCATTAC AACACAAGCT AGCATGGAGT TTCGACGGAA AGGGTCACAA
    ATGTCCACAG ACACCATGGT TTCCAATCCT ATGTTTGATG CAAGTGAATT TCCTGATAAC
    TATGAAGCAG GAAGAGCTGA GGCTTGTGGG ACACTGTGTA GGATTTTTTG TAGCAAGAAG
    ACTGGAGAAG AGATTCTGCC AGCTTATTTA TCCAGATTTT ACATGCTTTT AATTCAAGGT
    TTGCAGATAA ATGATTATGT GTGCCATCCT GTCTTGGCCA GCGTTATTCT AAACTCCCCT
    CCTTTGTTCT GCTGTGACTT GAAAGGGATT GATGTTGTGG TTCCTTACTT TATTTCAGCT
    CTTGAAACCA TTTTGCCTGA CAGAGAACTC TCAAAATTCA AAAGCTATGT AAATCCAACA
    GAATTGAGAA GATCTTCCAT TAATATCCTC CTTTCTTTAT TGCCCCTCCC TCATCATTTT
    GGCACAGTCA GATCTGAGGT GGTCCTGGAA GGGAAGTTTA GTAATGATGA CAGCTCTTCA
    TATGATAAAC CAATAACTTT TTTGTCCCTG AAGTTGAGAC TTGTGAATAT ACTAATAGGT
    GCCTTGCAAA CGGAAACGGA CCCCAACAAC ACTCAAATGA TACTAGGGGC AATGTTAAAT
    ATTGTTCAAG ATTCAGCACT TTTAGAAGCC ATTGGTTGCC AAATGGAGAT GGGTGGTGGA
    GAAAATAACC TGAAGAGTCA TAGTCGTACT AATAGTGGTA TTAGTTCAGC AAGTGGTGGA
    AGCACAGAAC CCACAACTCC TGATAGTGAA AGACCTGCTC AAGCTCTCCT AAGGGATTAT
    GCTCTTAATA CAGATTCAGC TGCTGGGCTC CTGATTCGCA GCATTCATCT TGTCACCCAA
    AGACTCAACT CTCAGTGGCG GCAAGACATG AGCATATCAC TGGCAGCGCT GGAGCTCCTC
    TCTGGCCTGG CAAAGGTGAA GGTGATGGTT GACTCGGGAG ACCGGAAACG AGCCATCAGT
    TCTGTGTGCA GCTACATCGT GTACCAATGT AGTCGGCCAG CTCCTCTTCA CTCCCGCGAT
    CTGCACTCCA TGATAGTAGC AGCTTTTCAG TGTCTCTGTG TCTGGCTGAC AGAGCATCCT
    GATATGCTTG ATGAAAAGGA CTGTCTTAAG GAAGTATTGG AGATTGTGGA ACTGGGTATT
    TCAGGAAGTA AGTCCAAGAA CAGTGAGCAA GAGGTCAAGT ACAAAGGAGA TAAGGAGCCA
    AACCCTGCAT CTATGAGGGT AAAGGATGCT GCTGAAGCCA CTCTAACATG TATTATGCAG
    TTGCTCGGCG CGTTTCCTTC ACCCAGTGGT CCTGCCTCTC CTTGTAGTCT GGTGAATGAG
    ACCACTTTGA TTAAATACTC AAGGCTGCCA ACCATAAACA AGCATAGTTT CCGGTACTTT
    GTCTTGGATA ACAGTGTCAT CCTGGCAATG CTGGAGCAAC CTCTTGGAAA TGAACAGAAT
    GATTTTTTTC CCTCTGTCAC TGTGCTGGTT CGAGGAATGT CTGGAAGACT TGCTTGGGCA
    CAACAACTTT GCCTTTTACC CAGAGGAGCA AAAGCAAATC AGAAGCTTTT TGTACCTGAA
    CCTCGTCCAA TTCCTAAAAA TGATGTTGGA TTTAAATATA CTGTCAAACA TCGACCATTT
    CCTGAAGAGG TGGATAAGAT TCCTTTTGTG AAAGCAGATC TGAGCATTCC AGATTTGCAT
    GAAATTGTCA CTGAAGAATT AGAAGAGAGA CATGAAAAAT TAAGGAATGG CATGGCCCAG
    CAGATTGCTT ATGAAATACA CCTTGAACAA CAAAGTGAGG AAGAATTGCA GAAGAGAAGT
    TTTCCTGATC CAATTACGGA TTGTAAGCCT CCACCTCCTG CCCAAGAATT CCAAACAGCA
    CGCCTCTTCC TTTCACACTT TGGATTTTTG TCTTTAGAAG CATTGAAGGA ACCTGCAAAT
    AGTCGCCTAC CTCCTCACCT TATTGCACTT GACTCCACGA TACCTGGATT TTTTGATGAC
    ATTGGATATC TGGATCTCTT GCCGTGTCGT CCCTTTGACA CAGTTTTTAT TTTCTATATG
    AAACCAGGTC AGAAAACAAA CCAGGAGATT TTAAAGAATG TGGAATCTTC CAGAAATGTT
    CAGCCACATT TCCTAGAATT TTTGCTCTCC CTTGGCTGGT CAGTAGATGT GGGCAGACAC
    CCAGGTTGGA CTGGGCATGT TTCTACCAGT TGGTCTATTA ACAGTTGTGA TGATGGTGAA
    GGACCTGAAC AAGATGAAGT GATTTCCTCT GAAGACATTG GGGCTAGCAT TTTCAATGGA
    CAGAAGAAGG TGCTGTATTA TGCTGATGCC CTTACGGAAA TAGCTTTTGT AGTTCCTTCT
    CCTGTGGAGT CTTTAACTGA TTCATTAGAA AGTAACATCT CGGACCAAGA TAGTGATTCA
    AATATGGATC TTATGCCTGG AATTCTGAAA CAGCCATCCC TGACACTTGA GCTTTTCCCT
    AATCACACAG ACAATCTTAA TTCCTCACAG CGGCTTAGTC CCAGTTCCAG AATGAAGAAG
    CTGCCTCAGG GTCGCCCTGT GCCTCCCCTT GGACCTGAGA CAAGAGTTTC TGTAGTCTGG
    GTGGAACGCT ATGATGATAT AGAGAACTTT CCCCTCTCAG ACCTGCTGAC AGAGATCAGT
    ACTGGTGTGG AAACTACTGC AAACAGCAGC ACTTCTCTGA GATCTACCAC TCTTGAAAAA
    GAAGTTCCTG TCATCTTTAT CCACCCTTTA AACACTGGAT TATTCCGGAT AAAAATTCAA
    GGAGCCACTG GAAAATTTAA CATGGTCATC CCGCTCGTGG ATGGGATGAT TGTCAGCAGA
    CGAGCACTTG GTTTTCTGGT GAGGCAGACT GTAATTAATA TTTGTAGAAG AAAGAGACTG
    GAGAGTGACT CCTACAGTCC ACCACATGTC CGCCGGAAAC AGAAAATCAC CGACATTGTC
    AACAAGTACC GGAACAAGCA GTTGGAGCCA GAGTTTTATA CTTCACTTTT CCAGGAGGTT
    GGACTCAAGA ACTGCTCTTC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005635108.1
    CDS333..4856
    Translation

    Target ORF information:

    RefSeq Version XM_005635051.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Ral GTPase activating protein, beta subunit (non-catalytic) (RALGAPB), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005635051.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATCCAGA ATGATCAGGG CCACACGAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGG CGAGAGGTGG CAAATGCAGT GGTCCATCCC
    CTTGGGCAGG CCTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAGTTTGTT AAAAACTGAC
    AAAGAAGTGA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGTGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTATTGCCG
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC TATATGTTCA AAGTATACTA
    AAACACCTGC AAAATCTTTT TGTACCAAGA CAGGAACAGG GCTCCAGTCA GATCCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTAGCCCGTG AGTCATCCAT CATGGCACGA
    GAAACGTGGG AAGTCTTACT ATTGTTTCTT CTGCAGATTA ATGACATACT TCTAGCCCCG
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTTTGGT TACTAGCTTG TACTCGGTGC TTTCCAACAC CTCCTTATTG GAAAACCGCC
    AAGGAGATGG TGGCAAACTG GAGGCACCAC CCGGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ACCTGTGCCC TCACTTCAAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTCCATTT
    AAAGTTCCTG ATGAAGATGC CAGTCTGATC CCTCCTGAAA TGGATAATGA GTGTGTAGCA
    CAGACATGGT TTCGCTTCTT ACATATGTTA AGTAATCCCG TGGATTTGAG TAACCCGGCC
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTAA ATGTGAGCGG AATGCCACAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTAGTGGA TGCATTCTTA GGCATTTCTA GACCCCGATC AGACAGTGCT
    CCTCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGCA CTGCTGTCAG TACCACCCCA
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC GAAAGTTCAG CACCAGACAT CCTCCACCTC TCCTCTGTCA
    AGTCCCAATC AGACTAGTTC TGAACCCCGG CCACTGCCTG CCCCTCGAAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCAT CTTTTATCCA AATTCTTCTT
    TCTTATAAAT CTTCCATTAC AACACAAGCT AGCATGGAGT TTCGACGGAA AGGGTCACAA
    ATGTCCACAG ACACCATGGT TTCCAATCCT ATGTTTGATG CAAGTGAATT TCCTGATAAC
    TATGAAGCAG GAAGAGCTGA GGCTTGTGGG ACACTGTGTA GGATTTTTTG TAGCAAGAAG
    ACTGGAGAAG AGATTCTGCC AGCTTATTTA TCCAGATTTT ACATGCTTTT AATTCAAGGT
    TTGCAGATAA ATGATTATGT GTGCCATCCT GTCTTGGCCA GCGTTATTCT AAACTCCCCT
    CCTTTGTTCT GCTGTGACTT GAAAGGGATT GATGTTGTGG TTCCTTACTT TATTTCAGCT
    CTTGAAACCA TTTTGCCTGA CAGAGAACTC TCAAAATTCA AAAGCTATGT AAATCCAACA
    GAATTGAGAA GATCTTCCAT TAATATCCTC CTTTCTTTAT TGCCCCTCCC TCATCATTTT
    GGCACAGTCA GATCTGAGGT GGTCCTGGAA GGGAAGTTTA GTAATGATGA CAGCTCTTCA
    TATGATAAAC CAATAACTTT TTTGTCCCTG AAGTTGAGAC TTGTGAATAT ACTAATAGGT
    GCCTTGCAAA CGGAAACGGA CCCCAACAAC ACTCAAATGA TACTAGGGGC AATGTTAAAT
    ATTGTTCAAG ATTCAGCACT TTTAGAAGCC ATTGGTTGCC AAATGGAGAT GGGTGGTGGA
    GAAAATAACC TGAAGAGTCA TAGTCGTACT AATAGTGGTA TTAGTTCAGC AAGTGGTGGA
    AGCACAGAAC CCACAACTCC TGATAGTGAA AGACCTGCTC AAGCTCTCCT AAGGGATTAT
    GCTCTTAATA CAGATTCAGC TGCTGGGCTC CTGATTCGCA GCATTCATCT TGTCACCCAA
    AGACTCAACT CTCAGTGGCG GCAAGACATG AGCATATCAC TGGCAGCGCT GGAGCTCCTC
    TCTGGCCTGG CAAAGGTGAA GGTGATGGTT GACTCGGGAG ACCGGAAACG AGCCATCAGT
    TCTGTGTGCA GCTACATCGT GTACCAATGT AGTCGGCCAG CTCCTCTTCA CTCCCGCGAT
    CTGCACTCCA TGATAGTAGC AGCTTTTCAG TGTCTCTGTG TCTGGCTGAC AGAGCATCCT
    GATATGCTTG ATGAAAAGGA CTGTCTTAAG GAAGTATTGG AGATTGTGGA ACTGGGTATT
    TCAGGAAGTA AGTCCAAGAA CAGTGAGCAA GAGGTCAAGT ACAAAGGAGA TAAGGAGCCA
    AACCCTGCAT CTATGAGGGT AAAGGATGCT GCTGAAGCCA CTCTAACATG TATTATGCAG
    TTGCTCGGCG CGTTTCCTTC ACCCAGTGGT CCTGCCTCTC CTTGTAGTCT GGTGAATGAG
    ACCACTTTGA TTAAATACTC AAGGCTGCCA ACCATAAACA AGCATAGTTT CCGGTACTTT
    GTCTTGGATA ACAGTGTCAT CCTGGCAATG CTGGAGCAAC CTCTTGGAAA TGAACAGAAT
    GATTTTTTTC CCTCTGTCAC TGTGCTGGTT CGAGGAATGT CTGGAAGACT TGCTTGGGCA
    CAACAACTTT GCCTTTTACC CAGAGGAGCA AAAGCAAATC AGAAGCTTTT TGTACCTGAA
    CCTCGTCCAA TTCCTAAAAA TGATGTTGGA TTTAAATATA CTGTCAAACA TCGACCATTT
    CCTGAAGAGG TGGATAAGAT TCCTTTTGTG AAAGCAGATC TGAGCATTCC AGATTTGCAT
    GAAATTGTCA CTGAAGAATT AGAAGAGAGA CATGAAAAAT TAAGGAATGG CATGGCCCAG
    CAGATTGCTT ATGAAATACA CCTTGAACAA CAAAGTGAGG AAGAATTGCA GAAGAGAAGT
    TTTCCTGATC CAATTACGGA TTGTAAGCCT CCACCTCCTG CCCAAGAATT CCAAACAGCA
    CGCCTCTTCC TTTCACACTT TGGATTTTTG TCTTTAGAAG CATTGAAGGA ACCTGCAAAT
    AGTCGCCTAC CTCCTCACCT TATTGCACTT GACTCCACGA TACCTGGATT TTTTGATGAC
    ATTGGATATC TGGATCTCTT GCCGTGTCGT CCCTTTGACA CAGTTTTTAT TTTCTATATG
    AAACCAGGTC AGAAAACAAA CCAGGAGATT TTAAAGAATG TGGAATCTTC CAGAAATGTT
    CAGCCACATT TCCTAGAATT TTTGCTCTCC CTTGGCTGGT CAGTAGATGT GGGCAGACAC
    CCAGGTTGGA CTGGGCATGT TTCTACCAGT TGGTCTATTA ACAGTTGTGA TGATGGTGAA
    GGACCTGAAC AAGATGAAGT GATTTCCTCT GAAGACATTG GGGCTAGCAT TTTCAATGGA
    CAGAAGAAGG TGCTGTATTA TGCTGATGCC CTTACGGAAA TAGCTTTTGT AGTTCCTTCT
    CCTGTGGAGT CTTTAACTGA TTCATTAGAA AGTAACATCT CGGACCAAGA TAGTGATTCA
    AATATGGATC TTATGCCTGG AATTCTGAAA CAGCCATCCC TGACACTTGA GCTTTTCCCT
    AATCACACAG ACAATCTTAA TTCCTCACAG CGGCTTAGTC CCAGTTCCAG AATGAAGAAG
    CTGCCTCAGG GTCGCCCTGT GCCTCCCCTT GGACCTGAGA CAAGAGTTTC TGTAGTCTGG
    GTGGAACGCT ATGATGATAT AGAGAACTTT CCCCTCTCAG ACCTGCTGAC AGAGATCAGT
    ACTGGTGTGG AAACTACTGC AAACAGCAGC ACTTCTCTGA GATCTACCAC TCTTGAAAAA
    GAAGTTCCTG TCATCTTTAT CCACCCTTTA AACACTGGAT TATTCCGGAT AAAAATTCAA
    GGAGCCACTG GAAAATTTAA CATGGTCATC CCGCTCGTGG ATGGGATGAT TGTCAGCAGA
    CGAGCACTTG GTTTTCTGGT GAGGCAGACT GTAATTAATA TTTGTAGAAG AAAGAGACTG
    GAGAGTGACT CCTACAGTCC ACCACATGTC CGCCGGAAAC AGAAAATCAC CGACATTGTC
    AACAAGTACC GGAACAAGCA GTTGGAGCCA GAGTTTTATA CTTCACTTTT CCAGGAGGTT
    GGACTCAAGA ACTGCTCTTC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe24890
    Clone ID Related Accession (Same CDS sequence) XM_022409443.1 , XM_005635052.2
    Accession Version XM_022409443.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4521bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ral GTPase-activating protein subunit beta isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATCCAGA ATGATCAGGG CCACACGAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGG CGAGAGGTGG CAAATGCAGT GGTCCATCCC
    CTTGGGCAGG CCTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAGTTTGTT AAAAACTGAC
    AAAGAAGTGA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGTGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTATTGCCG
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC TATATGTTCA AAGTATACTA
    AAACACCTGC AAAATCTTTT TGTACCAAGA CAGGAACAGG GCTCCAGTCA GATCCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTAGCCCGTG AGTCATCCAT CATGGCACGA
    GAAACGTGGG AAGTCTTACT ATTGTTTCTT CTGCAGATTA ATGACATACT TCTAGCCCCG
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTTTGGT TACTAGCTTG TACTCGGTGC TTTCCAACAC CTCCTTATTG GAAAACCGCC
    AAGGAGATGG TGGCAAACTG GAGGCACCAC CCGGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ACCTGTGCCC TCACTTCAAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTCCATTT
    AAAGTTCCTG ATGAAGATGC CAGTCTGATC CCTCCTGAAA TGGATAATGA GTGTGTAGCA
    CAGACATGGT TTCGCTTCTT ACATATGTTA AGTAATCCCG TGGATTTGAG TAACCCGGCC
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTAA ATGTGAGCGG AATGCCACAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTAGTGGA TGCATTCTTA GGCATTTCTA GACCCCGATC AGACAGTGCT
    CCTCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGCA CTGCTGTCAG TACCACCCCA
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC GAAAGTTCAG CACCAGACAT CCTCCACCTC TCCTCTGTCA
    AGTCCCAATC AGACTAGTTC TGAACCCCGG CCACTGCCTG CCCCTCGAAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCAT CTTTTATCCA AATTCTTCTT
    TCTTATAAAT CTTCCATTAC AACACAAGCT AGCATGGAGT TTCGACGGAA AGGGTCACAA
    ATGTCCACAG ACACCATGGT TTCCAATCCT ATGTTTGATG CAAGTGAATT TCCTGATAAC
    TATGAAGCAG GAAGAGCTGA GGCTTGTGGG ACACTGTGTA GGATTTTTTG TAGCAAGAAG
    ACTGGAGAAG AGATTCTGCC AGCTTATTTA TCCAGATTTT ACATGCTTTT AATTCAAGGT
    TTGCAGATAA ATGATTATGT GTGCCATCCT GTCTTGGCCA GCGTTATTCT AAACTCCCCT
    CCTTTGTTCT GCTGTGACTT GAAAGGGATT GATGTTGTGG TTCCTTACTT TATTTCAGCT
    CTTGAAACCA TTTTGCCTGA CAGAGAACTC TCAAAATTCA AAAGCTATGT AAATCCAACA
    GAATTGAGAA GATCTTCCAT TAATATCCTC CTTTCTTTAT TGCCCCTCCC TCATCATTTT
    GGCACAGTCA GATCTGAGGT GGTCCTGGAA GGGAAGTTTA GTAATGATGA CAGCTCTTCA
    TATGATAAAC CAATAACTTT TTTGTCCCTG AAGTTGAGAC TTGTGAATAT ACTAATAGGT
    GCCTTGCAAA CGGAAACGGA CCCCAACAAC ACTCAAATGA TACTAGGGGC AATGTTAAAT
    ATTGTTCAAG ATTCAGCACT TTTAGAAGCC ATTGGTTGCC AAATGGAGAT GGGTGGTGGA
    GAAAATAACC TGAAGAGTCA TAGTCGTACT AATAGTGGTA TTAGTTCAGC AAGTGGTGGA
    AGCACAGAAC CCACAACTCC TGATAGTGAA AGACCTGCTC AAGCTCTCCT AAGGGATTAT
    GGATCGACAG ATTCAGCTGC TGGGCTCCTG ATTCGCAGCA TTCATCTTGT CACCCAAAGA
    CTCAACTCTC AGTGGCGGCA AGACATGAGC ATATCACTGG CAGCGCTGGA GCTCCTCTCT
    GGCCTGGCAA AGGTGAAGGT GATGGTTGAC TCGGGAGACC GGAAACGAGC CATCAGTTCT
    GTGTGCAGCT ACATCGTGTA CCAATGTAGT CGGCCAGCTC CTCTTCACTC CCGCGATCTG
    CACTCCATGA TAGTAGCAGC TTTTCAGTGT CTCTGTGTCT GGCTGACAGA GCATCCTGAT
    ATGCTTGATG AAAAGGACTG TCTTAAGGAA GTATTGGAGA TTGTGGAACT GGGTATTTCA
    GGAAGTAAGT CCAAGAACAG TGAGCAAGAG GTCAAGTACA AAGGAGATAA GGAGCCAAAC
    CCTGCATCTA TGAGGGTAAA GGATGCTGCT GAAGCCACTC TAACATGTAT TATGCAGTTG
    CTCGGCGCGT TTCCTTCACC CAGTGGTCCT GCCTCTCCTT GTAGTCTGGT GAATGAGACC
    ACTTTGATTA AATACTCAAG GCTGCCAACC ATAAACAAGC ATAGTTTCCG GTACTTTGTC
    TTGGATAACA GTGTCATCCT GGCAATGCTG GAGCAACCTC TTGGAAATGA ACAGAATGAT
    TTTTTTCCCT CTGTCACTGT GCTGGTTCGA GGAATGTCTG GAAGACTTGC TTGGGCACAA
    CAACTTTGCC TTTTACCCAG AGGAGCAAAA GCAAATCAGA AGCTTTTTGT ACCTGAACCT
    CGTCCAATTC CTAAAAATGA TGTTGGATTT AAATATACTG TCAAACATCG ACCATTTCCT
    GAAGAGGTGG ATAAGATTCC TTTTGTGAAA GCAGATCTGA GCATTCCAGA TTTGCATGAA
    ATTGTCACTG AAGAATTAGA AGAGAGACAT GAAAAATTAA GGAATGGCAT GGCCCAGCAG
    ATTGCTTATG AAATACACCT TGAACAACAA AGTGAGGAAG AATTGCAGAA GAGAAGTTTT
    CCTGATCCAA TTACGGATTG TAAGCCTCCA CCTCCTGCCC AAGAATTCCA AACAGCACGC
    CTCTTCCTTT CACACTTTGG ATTTTTGTCT TTAGAAGCAT TGAAGGAACC TGCAAATAGT
    CGCCTACCTC CTCACCTTAT TGCACTTGAC TCCACGATAC CTGGATTTTT TGATGACATT
    GGATATCTGG ATCTCTTGCC GTGTCGTCCC TTTGACACAG TTTTTATTTT CTATATGAAA
    CCAGGTCAGA AAACAAACCA GGAGATTTTA AAGAATGTGG AATCTTCCAG AAATGTTCAG
    CCACATTTCC TAGAATTTTT GCTCTCCCTT GGCTGGTCAG TAGATGTGGG CAGACACCCA
    GGTTGGACTG GGCATGTTTC TACCAGTTGG TCTATTAACA GTTGTGATGA TGGTGAAGGA
    CCTGAACAAG ATGAAGTGAT TTCCTCTGAA GACATTGGGG CTAGCATTTT CAATGGACAG
    AAGAAGGTGC TGTATTATGC TGATGCCCTT ACGGAAATAG CTTTTGTAGT TCCTTCTCCT
    GTGGAGTCTT TAACTGATTC ATTAGAAAGT AACATCTCGG ACCAAGATAG TGATTCAAAT
    ATGGATCTTA TGCCTGGAAT TCTGAAACAG CCATCCCTGA CACTTGAGCT TTTCCCTAAT
    CACACAGACA ATCTTAATTC CTCACAGCGG CTTAGTCCCA GTTCCAGAAT GAAGAAGCTG
    CCTCAGGGTC GCCCTGTGCC TCCCCTTGGA CCTGAGACAA GAGTTTCTGT AGTCTGGGTG
    GAACGCTATG ATGATATAGA GAACTTTCCC CTCTCAGACC TGCTGACAGA GATCAGTACT
    GGTGTGGAAA CTACTGCAAA CAGCAGCACT TCTCTGAGAT CTACCACTCT TGAAAAAGAA
    GTTCCTGTCA TCTTTATCCA CCCTTTAAAC ACTGGATTAT TCCGGATAAA AATTCAAGGA
    GCCACTGGAA AATTTAACAT GGTCATCCCG CTCGTGGATG GGATGATTGT CAGCAGACGA
    GCACTTGGTT TTCTGGTGAG GCAGACTGTA ATTAATATTT GTAGAAGAAA GAGACTGGAG
    AGTGACTCCT ACAGTCCACC ACATGTCCGC CGGAAACAGA AAATCACCGA CATTGTCAAC
    AAGTACCGGA ACAAGCAGTT GGAGCCAGAG TTTTATACTT CACTTTTCCA GGAGGTTGGA
    CTCAAGAACT GCTCTTCTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022265151.1
    CDS370..4890
    Translation

    Target ORF information:

    RefSeq Version XM_022409443.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022409443.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATCCAGA ATGATCAGGG CCACACGAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGG CGAGAGGTGG CAAATGCAGT GGTCCATCCC
    CTTGGGCAGG CCTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAGTTTGTT AAAAACTGAC
    AAAGAAGTGA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGTGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTATTGCCG
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC TATATGTTCA AAGTATACTA
    AAACACCTGC AAAATCTTTT TGTACCAAGA CAGGAACAGG GCTCCAGTCA GATCCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTAGCCCGTG AGTCATCCAT CATGGCACGA
    GAAACGTGGG AAGTCTTACT ATTGTTTCTT CTGCAGATTA ATGACATACT TCTAGCCCCG
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTTTGGT TACTAGCTTG TACTCGGTGC TTTCCAACAC CTCCTTATTG GAAAACCGCC
    AAGGAGATGG TGGCAAACTG GAGGCACCAC CCGGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ACCTGTGCCC TCACTTCAAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTCCATTT
    AAAGTTCCTG ATGAAGATGC CAGTCTGATC CCTCCTGAAA TGGATAATGA GTGTGTAGCA
    CAGACATGGT TTCGCTTCTT ACATATGTTA AGTAATCCCG TGGATTTGAG TAACCCGGCC
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTAA ATGTGAGCGG AATGCCACAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTAGTGGA TGCATTCTTA GGCATTTCTA GACCCCGATC AGACAGTGCT
    CCTCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGCA CTGCTGTCAG TACCACCCCA
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC GAAAGTTCAG CACCAGACAT CCTCCACCTC TCCTCTGTCA
    AGTCCCAATC AGACTAGTTC TGAACCCCGG CCACTGCCTG CCCCTCGAAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCAT CTTTTATCCA AATTCTTCTT
    TCTTATAAAT CTTCCATTAC AACACAAGCT AGCATGGAGT TTCGACGGAA AGGGTCACAA
    ATGTCCACAG ACACCATGGT TTCCAATCCT ATGTTTGATG CAAGTGAATT TCCTGATAAC
    TATGAAGCAG GAAGAGCTGA GGCTTGTGGG ACACTGTGTA GGATTTTTTG TAGCAAGAAG
    ACTGGAGAAG AGATTCTGCC AGCTTATTTA TCCAGATTTT ACATGCTTTT AATTCAAGGT
    TTGCAGATAA ATGATTATGT GTGCCATCCT GTCTTGGCCA GCGTTATTCT AAACTCCCCT
    CCTTTGTTCT GCTGTGACTT GAAAGGGATT GATGTTGTGG TTCCTTACTT TATTTCAGCT
    CTTGAAACCA TTTTGCCTGA CAGAGAACTC TCAAAATTCA AAAGCTATGT AAATCCAACA
    GAATTGAGAA GATCTTCCAT TAATATCCTC CTTTCTTTAT TGCCCCTCCC TCATCATTTT
    GGCACAGTCA GATCTGAGGT GGTCCTGGAA GGGAAGTTTA GTAATGATGA CAGCTCTTCA
    TATGATAAAC CAATAACTTT TTTGTCCCTG AAGTTGAGAC TTGTGAATAT ACTAATAGGT
    GCCTTGCAAA CGGAAACGGA CCCCAACAAC ACTCAAATGA TACTAGGGGC AATGTTAAAT
    ATTGTTCAAG ATTCAGCACT TTTAGAAGCC ATTGGTTGCC AAATGGAGAT GGGTGGTGGA
    GAAAATAACC TGAAGAGTCA TAGTCGTACT AATAGTGGTA TTAGTTCAGC AAGTGGTGGA
    AGCACAGAAC CCACAACTCC TGATAGTGAA AGACCTGCTC AAGCTCTCCT AAGGGATTAT
    GGATCGACAG ATTCAGCTGC TGGGCTCCTG ATTCGCAGCA TTCATCTTGT CACCCAAAGA
    CTCAACTCTC AGTGGCGGCA AGACATGAGC ATATCACTGG CAGCGCTGGA GCTCCTCTCT
    GGCCTGGCAA AGGTGAAGGT GATGGTTGAC TCGGGAGACC GGAAACGAGC CATCAGTTCT
    GTGTGCAGCT ACATCGTGTA CCAATGTAGT CGGCCAGCTC CTCTTCACTC CCGCGATCTG
    CACTCCATGA TAGTAGCAGC TTTTCAGTGT CTCTGTGTCT GGCTGACAGA GCATCCTGAT
    ATGCTTGATG AAAAGGACTG TCTTAAGGAA GTATTGGAGA TTGTGGAACT GGGTATTTCA
    GGAAGTAAGT CCAAGAACAG TGAGCAAGAG GTCAAGTACA AAGGAGATAA GGAGCCAAAC
    CCTGCATCTA TGAGGGTAAA GGATGCTGCT GAAGCCACTC TAACATGTAT TATGCAGTTG
    CTCGGCGCGT TTCCTTCACC CAGTGGTCCT GCCTCTCCTT GTAGTCTGGT GAATGAGACC
    ACTTTGATTA AATACTCAAG GCTGCCAACC ATAAACAAGC ATAGTTTCCG GTACTTTGTC
    TTGGATAACA GTGTCATCCT GGCAATGCTG GAGCAACCTC TTGGAAATGA ACAGAATGAT
    TTTTTTCCCT CTGTCACTGT GCTGGTTCGA GGAATGTCTG GAAGACTTGC TTGGGCACAA
    CAACTTTGCC TTTTACCCAG AGGAGCAAAA GCAAATCAGA AGCTTTTTGT ACCTGAACCT
    CGTCCAATTC CTAAAAATGA TGTTGGATTT AAATATACTG TCAAACATCG ACCATTTCCT
    GAAGAGGTGG ATAAGATTCC TTTTGTGAAA GCAGATCTGA GCATTCCAGA TTTGCATGAA
    ATTGTCACTG AAGAATTAGA AGAGAGACAT GAAAAATTAA GGAATGGCAT GGCCCAGCAG
    ATTGCTTATG AAATACACCT TGAACAACAA AGTGAGGAAG AATTGCAGAA GAGAAGTTTT
    CCTGATCCAA TTACGGATTG TAAGCCTCCA CCTCCTGCCC AAGAATTCCA AACAGCACGC
    CTCTTCCTTT CACACTTTGG ATTTTTGTCT TTAGAAGCAT TGAAGGAACC TGCAAATAGT
    CGCCTACCTC CTCACCTTAT TGCACTTGAC TCCACGATAC CTGGATTTTT TGATGACATT
    GGATATCTGG ATCTCTTGCC GTGTCGTCCC TTTGACACAG TTTTTATTTT CTATATGAAA
    CCAGGTCAGA AAACAAACCA GGAGATTTTA AAGAATGTGG AATCTTCCAG AAATGTTCAG
    CCACATTTCC TAGAATTTTT GCTCTCCCTT GGCTGGTCAG TAGATGTGGG CAGACACCCA
    GGTTGGACTG GGCATGTTTC TACCAGTTGG TCTATTAACA GTTGTGATGA TGGTGAAGGA
    CCTGAACAAG ATGAAGTGAT TTCCTCTGAA GACATTGGGG CTAGCATTTT CAATGGACAG
    AAGAAGGTGC TGTATTATGC TGATGCCCTT ACGGAAATAG CTTTTGTAGT TCCTTCTCCT
    GTGGAGTCTT TAACTGATTC ATTAGAAAGT AACATCTCGG ACCAAGATAG TGATTCAAAT
    ATGGATCTTA TGCCTGGAAT TCTGAAACAG CCATCCCTGA CACTTGAGCT TTTCCCTAAT
    CACACAGACA ATCTTAATTC CTCACAGCGG CTTAGTCCCA GTTCCAGAAT GAAGAAGCTG
    CCTCAGGGTC GCCCTGTGCC TCCCCTTGGA CCTGAGACAA GAGTTTCTGT AGTCTGGGTG
    GAACGCTATG ATGATATAGA GAACTTTCCC CTCTCAGACC TGCTGACAGA GATCAGTACT
    GGTGTGGAAA CTACTGCAAA CAGCAGCACT TCTCTGAGAT CTACCACTCT TGAAAAAGAA
    GTTCCTGTCA TCTTTATCCA CCCTTTAAAC ACTGGATTAT TCCGGATAAA AATTCAAGGA
    GCCACTGGAA AATTTAACAT GGTCATCCCG CTCGTGGATG GGATGATTGT CAGCAGACGA
    GCACTTGGTT TTCTGGTGAG GCAGACTGTA ATTAATATTT GTAGAAGAAA GAGACTGGAG
    AGTGACTCCT ACAGTCCACC ACATGTCCGC CGGAAACAGA AAATCACCGA CATTGTCAAC
    AAGTACCGGA ACAAGCAGTT GGAGCCAGAG TTTTATACTT CACTTTTCCA GGAGGTTGGA
    CTCAAGAACT GCTCTTCTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe24890
    Clone ID Related Accession (Same CDS sequence) XM_022409443.1 , XM_005635052.2
    Accession Version XM_005635052.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4521bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ral GTPase-activating protein subunit beta isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726101.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005635052.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATCCAGA ATGATCAGGG CCACACGAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGG CGAGAGGTGG CAAATGCAGT GGTCCATCCC
    CTTGGGCAGG CCTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAGTTTGTT AAAAACTGAC
    AAAGAAGTGA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGTGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTATTGCCG
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC TATATGTTCA AAGTATACTA
    AAACACCTGC AAAATCTTTT TGTACCAAGA CAGGAACAGG GCTCCAGTCA GATCCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTAGCCCGTG AGTCATCCAT CATGGCACGA
    GAAACGTGGG AAGTCTTACT ATTGTTTCTT CTGCAGATTA ATGACATACT TCTAGCCCCG
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTTTGGT TACTAGCTTG TACTCGGTGC TTTCCAACAC CTCCTTATTG GAAAACCGCC
    AAGGAGATGG TGGCAAACTG GAGGCACCAC CCGGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ACCTGTGCCC TCACTTCAAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTCCATTT
    AAAGTTCCTG ATGAAGATGC CAGTCTGATC CCTCCTGAAA TGGATAATGA GTGTGTAGCA
    CAGACATGGT TTCGCTTCTT ACATATGTTA AGTAATCCCG TGGATTTGAG TAACCCGGCC
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTAA ATGTGAGCGG AATGCCACAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTAGTGGA TGCATTCTTA GGCATTTCTA GACCCCGATC AGACAGTGCT
    CCTCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGCA CTGCTGTCAG TACCACCCCA
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC GAAAGTTCAG CACCAGACAT CCTCCACCTC TCCTCTGTCA
    AGTCCCAATC AGACTAGTTC TGAACCCCGG CCACTGCCTG CCCCTCGAAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCAT CTTTTATCCA AATTCTTCTT
    TCTTATAAAT CTTCCATTAC AACACAAGCT AGCATGGAGT TTCGACGGAA AGGGTCACAA
    ATGTCCACAG ACACCATGGT TTCCAATCCT ATGTTTGATG CAAGTGAATT TCCTGATAAC
    TATGAAGCAG GAAGAGCTGA GGCTTGTGGG ACACTGTGTA GGATTTTTTG TAGCAAGAAG
    ACTGGAGAAG AGATTCTGCC AGCTTATTTA TCCAGATTTT ACATGCTTTT AATTCAAGGT
    TTGCAGATAA ATGATTATGT GTGCCATCCT GTCTTGGCCA GCGTTATTCT AAACTCCCCT
    CCTTTGTTCT GCTGTGACTT GAAAGGGATT GATGTTGTGG TTCCTTACTT TATTTCAGCT
    CTTGAAACCA TTTTGCCTGA CAGAGAACTC TCAAAATTCA AAAGCTATGT AAATCCAACA
    GAATTGAGAA GATCTTCCAT TAATATCCTC CTTTCTTTAT TGCCCCTCCC TCATCATTTT
    GGCACAGTCA GATCTGAGGT GGTCCTGGAA GGGAAGTTTA GTAATGATGA CAGCTCTTCA
    TATGATAAAC CAATAACTTT TTTGTCCCTG AAGTTGAGAC TTGTGAATAT ACTAATAGGT
    GCCTTGCAAA CGGAAACGGA CCCCAACAAC ACTCAAATGA TACTAGGGGC AATGTTAAAT
    ATTGTTCAAG ATTCAGCACT TTTAGAAGCC ATTGGTTGCC AAATGGAGAT GGGTGGTGGA
    GAAAATAACC TGAAGAGTCA TAGTCGTACT AATAGTGGTA TTAGTTCAGC AAGTGGTGGA
    AGCACAGAAC CCACAACTCC TGATAGTGAA AGACCTGCTC AAGCTCTCCT AAGGGATTAT
    GGATCGACAG ATTCAGCTGC TGGGCTCCTG ATTCGCAGCA TTCATCTTGT CACCCAAAGA
    CTCAACTCTC AGTGGCGGCA AGACATGAGC ATATCACTGG CAGCGCTGGA GCTCCTCTCT
    GGCCTGGCAA AGGTGAAGGT GATGGTTGAC TCGGGAGACC GGAAACGAGC CATCAGTTCT
    GTGTGCAGCT ACATCGTGTA CCAATGTAGT CGGCCAGCTC CTCTTCACTC CCGCGATCTG
    CACTCCATGA TAGTAGCAGC TTTTCAGTGT CTCTGTGTCT GGCTGACAGA GCATCCTGAT
    ATGCTTGATG AAAAGGACTG TCTTAAGGAA GTATTGGAGA TTGTGGAACT GGGTATTTCA
    GGAAGTAAGT CCAAGAACAG TGAGCAAGAG GTCAAGTACA AAGGAGATAA GGAGCCAAAC
    CCTGCATCTA TGAGGGTAAA GGATGCTGCT GAAGCCACTC TAACATGTAT TATGCAGTTG
    CTCGGCGCGT TTCCTTCACC CAGTGGTCCT GCCTCTCCTT GTAGTCTGGT GAATGAGACC
    ACTTTGATTA AATACTCAAG GCTGCCAACC ATAAACAAGC ATAGTTTCCG GTACTTTGTC
    TTGGATAACA GTGTCATCCT GGCAATGCTG GAGCAACCTC TTGGAAATGA ACAGAATGAT
    TTTTTTCCCT CTGTCACTGT GCTGGTTCGA GGAATGTCTG GAAGACTTGC TTGGGCACAA
    CAACTTTGCC TTTTACCCAG AGGAGCAAAA GCAAATCAGA AGCTTTTTGT ACCTGAACCT
    CGTCCAATTC CTAAAAATGA TGTTGGATTT AAATATACTG TCAAACATCG ACCATTTCCT
    GAAGAGGTGG ATAAGATTCC TTTTGTGAAA GCAGATCTGA GCATTCCAGA TTTGCATGAA
    ATTGTCACTG AAGAATTAGA AGAGAGACAT GAAAAATTAA GGAATGGCAT GGCCCAGCAG
    ATTGCTTATG AAATACACCT TGAACAACAA AGTGAGGAAG AATTGCAGAA GAGAAGTTTT
    CCTGATCCAA TTACGGATTG TAAGCCTCCA CCTCCTGCCC AAGAATTCCA AACAGCACGC
    CTCTTCCTTT CACACTTTGG ATTTTTGTCT TTAGAAGCAT TGAAGGAACC TGCAAATAGT
    CGCCTACCTC CTCACCTTAT TGCACTTGAC TCCACGATAC CTGGATTTTT TGATGACATT
    GGATATCTGG ATCTCTTGCC GTGTCGTCCC TTTGACACAG TTTTTATTTT CTATATGAAA
    CCAGGTCAGA AAACAAACCA GGAGATTTTA AAGAATGTGG AATCTTCCAG AAATGTTCAG
    CCACATTTCC TAGAATTTTT GCTCTCCCTT GGCTGGTCAG TAGATGTGGG CAGACACCCA
    GGTTGGACTG GGCATGTTTC TACCAGTTGG TCTATTAACA GTTGTGATGA TGGTGAAGGA
    CCTGAACAAG ATGAAGTGAT TTCCTCTGAA GACATTGGGG CTAGCATTTT CAATGGACAG
    AAGAAGGTGC TGTATTATGC TGATGCCCTT ACGGAAATAG CTTTTGTAGT TCCTTCTCCT
    GTGGAGTCTT TAACTGATTC ATTAGAAAGT AACATCTCGG ACCAAGATAG TGATTCAAAT
    ATGGATCTTA TGCCTGGAAT TCTGAAACAG CCATCCCTGA CACTTGAGCT TTTCCCTAAT
    CACACAGACA ATCTTAATTC CTCACAGCGG CTTAGTCCCA GTTCCAGAAT GAAGAAGCTG
    CCTCAGGGTC GCCCTGTGCC TCCCCTTGGA CCTGAGACAA GAGTTTCTGT AGTCTGGGTG
    GAACGCTATG ATGATATAGA GAACTTTCCC CTCTCAGACC TGCTGACAGA GATCAGTACT
    GGTGTGGAAA CTACTGCAAA CAGCAGCACT TCTCTGAGAT CTACCACTCT TGAAAAAGAA
    GTTCCTGTCA TCTTTATCCA CCCTTTAAAC ACTGGATTAT TCCGGATAAA AATTCAAGGA
    GCCACTGGAA AATTTAACAT GGTCATCCCG CTCGTGGATG GGATGATTGT CAGCAGACGA
    GCACTTGGTT TTCTGGTGAG GCAGACTGTA ATTAATATTT GTAGAAGAAA GAGACTGGAG
    AGTGACTCCT ACAGTCCACC ACATGTCCGC CGGAAACAGA AAATCACCGA CATTGTCAAC
    AAGTACCGGA ACAAGCAGTT GGAGCCAGAG TTTTATACTT CACTTTTCCA GGAGGTTGGA
    CTCAAGAACT GCTCTTCTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005635109.1
    CDS333..4853
    Translation

    Target ORF information:

    RefSeq Version XM_005635052.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Ral GTPase activating protein, beta subunit (non-catalytic) (RALGAPB), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005635052.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATCCAGA ATGATCAGGG CCACACGAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGG CGAGAGGTGG CAAATGCAGT GGTCCATCCC
    CTTGGGCAGG CCTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAGTTTGTT AAAAACTGAC
    AAAGAAGTGA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGTGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTATTGCCG
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC TATATGTTCA AAGTATACTA
    AAACACCTGC AAAATCTTTT TGTACCAAGA CAGGAACAGG GCTCCAGTCA GATCCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTAGCCCGTG AGTCATCCAT CATGGCACGA
    GAAACGTGGG AAGTCTTACT ATTGTTTCTT CTGCAGATTA ATGACATACT TCTAGCCCCG
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTTTGGT TACTAGCTTG TACTCGGTGC TTTCCAACAC CTCCTTATTG GAAAACCGCC
    AAGGAGATGG TGGCAAACTG GAGGCACCAC CCGGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ACCTGTGCCC TCACTTCAAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTCCATTT
    AAAGTTCCTG ATGAAGATGC CAGTCTGATC CCTCCTGAAA TGGATAATGA GTGTGTAGCA
    CAGACATGGT TTCGCTTCTT ACATATGTTA AGTAATCCCG TGGATTTGAG TAACCCGGCC
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTAA ATGTGAGCGG AATGCCACAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTAGTGGA TGCATTCTTA GGCATTTCTA GACCCCGATC AGACAGTGCT
    CCTCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGCA CTGCTGTCAG TACCACCCCA
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC GAAAGTTCAG CACCAGACAT CCTCCACCTC TCCTCTGTCA
    AGTCCCAATC AGACTAGTTC TGAACCCCGG CCACTGCCTG CCCCTCGAAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCAT CTTTTATCCA AATTCTTCTT
    TCTTATAAAT CTTCCATTAC AACACAAGCT AGCATGGAGT TTCGACGGAA AGGGTCACAA
    ATGTCCACAG ACACCATGGT TTCCAATCCT ATGTTTGATG CAAGTGAATT TCCTGATAAC
    TATGAAGCAG GAAGAGCTGA GGCTTGTGGG ACACTGTGTA GGATTTTTTG TAGCAAGAAG
    ACTGGAGAAG AGATTCTGCC AGCTTATTTA TCCAGATTTT ACATGCTTTT AATTCAAGGT
    TTGCAGATAA ATGATTATGT GTGCCATCCT GTCTTGGCCA GCGTTATTCT AAACTCCCCT
    CCTTTGTTCT GCTGTGACTT GAAAGGGATT GATGTTGTGG TTCCTTACTT TATTTCAGCT
    CTTGAAACCA TTTTGCCTGA CAGAGAACTC TCAAAATTCA AAAGCTATGT AAATCCAACA
    GAATTGAGAA GATCTTCCAT TAATATCCTC CTTTCTTTAT TGCCCCTCCC TCATCATTTT
    GGCACAGTCA GATCTGAGGT GGTCCTGGAA GGGAAGTTTA GTAATGATGA CAGCTCTTCA
    TATGATAAAC CAATAACTTT TTTGTCCCTG AAGTTGAGAC TTGTGAATAT ACTAATAGGT
    GCCTTGCAAA CGGAAACGGA CCCCAACAAC ACTCAAATGA TACTAGGGGC AATGTTAAAT
    ATTGTTCAAG ATTCAGCACT TTTAGAAGCC ATTGGTTGCC AAATGGAGAT GGGTGGTGGA
    GAAAATAACC TGAAGAGTCA TAGTCGTACT AATAGTGGTA TTAGTTCAGC AAGTGGTGGA
    AGCACAGAAC CCACAACTCC TGATAGTGAA AGACCTGCTC AAGCTCTCCT AAGGGATTAT
    GGATCGACAG ATTCAGCTGC TGGGCTCCTG ATTCGCAGCA TTCATCTTGT CACCCAAAGA
    CTCAACTCTC AGTGGCGGCA AGACATGAGC ATATCACTGG CAGCGCTGGA GCTCCTCTCT
    GGCCTGGCAA AGGTGAAGGT GATGGTTGAC TCGGGAGACC GGAAACGAGC CATCAGTTCT
    GTGTGCAGCT ACATCGTGTA CCAATGTAGT CGGCCAGCTC CTCTTCACTC CCGCGATCTG
    CACTCCATGA TAGTAGCAGC TTTTCAGTGT CTCTGTGTCT GGCTGACAGA GCATCCTGAT
    ATGCTTGATG AAAAGGACTG TCTTAAGGAA GTATTGGAGA TTGTGGAACT GGGTATTTCA
    GGAAGTAAGT CCAAGAACAG TGAGCAAGAG GTCAAGTACA AAGGAGATAA GGAGCCAAAC
    CCTGCATCTA TGAGGGTAAA GGATGCTGCT GAAGCCACTC TAACATGTAT TATGCAGTTG
    CTCGGCGCGT TTCCTTCACC CAGTGGTCCT GCCTCTCCTT GTAGTCTGGT GAATGAGACC
    ACTTTGATTA AATACTCAAG GCTGCCAACC ATAAACAAGC ATAGTTTCCG GTACTTTGTC
    TTGGATAACA GTGTCATCCT GGCAATGCTG GAGCAACCTC TTGGAAATGA ACAGAATGAT
    TTTTTTCCCT CTGTCACTGT GCTGGTTCGA GGAATGTCTG GAAGACTTGC TTGGGCACAA
    CAACTTTGCC TTTTACCCAG AGGAGCAAAA GCAAATCAGA AGCTTTTTGT ACCTGAACCT
    CGTCCAATTC CTAAAAATGA TGTTGGATTT AAATATACTG TCAAACATCG ACCATTTCCT
    GAAGAGGTGG ATAAGATTCC TTTTGTGAAA GCAGATCTGA GCATTCCAGA TTTGCATGAA
    ATTGTCACTG AAGAATTAGA AGAGAGACAT GAAAAATTAA GGAATGGCAT GGCCCAGCAG
    ATTGCTTATG AAATACACCT TGAACAACAA AGTGAGGAAG AATTGCAGAA GAGAAGTTTT
    CCTGATCCAA TTACGGATTG TAAGCCTCCA CCTCCTGCCC AAGAATTCCA AACAGCACGC
    CTCTTCCTTT CACACTTTGG ATTTTTGTCT TTAGAAGCAT TGAAGGAACC TGCAAATAGT
    CGCCTACCTC CTCACCTTAT TGCACTTGAC TCCACGATAC CTGGATTTTT TGATGACATT
    GGATATCTGG ATCTCTTGCC GTGTCGTCCC TTTGACACAG TTTTTATTTT CTATATGAAA
    CCAGGTCAGA AAACAAACCA GGAGATTTTA AAGAATGTGG AATCTTCCAG AAATGTTCAG
    CCACATTTCC TAGAATTTTT GCTCTCCCTT GGCTGGTCAG TAGATGTGGG CAGACACCCA
    GGTTGGACTG GGCATGTTTC TACCAGTTGG TCTATTAACA GTTGTGATGA TGGTGAAGGA
    CCTGAACAAG ATGAAGTGAT TTCCTCTGAA GACATTGGGG CTAGCATTTT CAATGGACAG
    AAGAAGGTGC TGTATTATGC TGATGCCCTT ACGGAAATAG CTTTTGTAGT TCCTTCTCCT
    GTGGAGTCTT TAACTGATTC ATTAGAAAGT AACATCTCGG ACCAAGATAG TGATTCAAAT
    ATGGATCTTA TGCCTGGAAT TCTGAAACAG CCATCCCTGA CACTTGAGCT TTTCCCTAAT
    CACACAGACA ATCTTAATTC CTCACAGCGG CTTAGTCCCA GTTCCAGAAT GAAGAAGCTG
    CCTCAGGGTC GCCCTGTGCC TCCCCTTGGA CCTGAGACAA GAGTTTCTGT AGTCTGGGTG
    GAACGCTATG ATGATATAGA GAACTTTCCC CTCTCAGACC TGCTGACAGA GATCAGTACT
    GGTGTGGAAA CTACTGCAAA CAGCAGCACT TCTCTGAGAT CTACCACTCT TGAAAAAGAA
    GTTCCTGTCA TCTTTATCCA CCCTTTAAAC ACTGGATTAT TCCGGATAAA AATTCAAGGA
    GCCACTGGAA AATTTAACAT GGTCATCCCG CTCGTGGATG GGATGATTGT CAGCAGACGA
    GCACTTGGTT TTCTGGTGAG GCAGACTGTA ATTAATATTT GTAGAAGAAA GAGACTGGAG
    AGTGACTCCT ACAGTCCACC ACATGTCCGC CGGAAACAGA AAATCACCGA CATTGTCAAC
    AAGTACCGGA ACAAGCAGTT GGAGCCAGAG TTTTATACTT CACTTTTCCA GGAGGTTGGA
    CTCAAGAACT GCTCTTCTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe24891
    Clone ID Related Accession (Same CDS sequence) XM_022409444.1 , XM_005635053.2
    Accession Version XM_022409444.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4512bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ral GTPase-activating protein subunit beta isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATCCAGA ATGATCAGGG CCACACGAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGG CGAGAGGTGG CAAATGCAGT GGTCCATCCC
    CTTGGGCAGG CCTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAGTTTGTT AAAAACTGAC
    AAAGAAGTGA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGTGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTATTGCCG
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC TATATGTTCA AAGTATACTA
    AAACACCTGC AAAATCTTTT TGTACCAAGA CAGGAACAGG GCTCCAGTCA GATCCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTAGCCCGTG AGTCATCCAT CATGGCACGA
    GAAACGTGGG AAGTCTTACT ATTGTTTCTT CTGCAGATTA ATGACATACT TCTAGCCCCG
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTTTGGT TACTAGCTTG TACTCGGTGC TTTCCAACAC CTCCTTATTG GAAAACCGCC
    AAGGAGATGG TGGCAAACTG GAGGCACCAC CCGGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ACCTGTGCCC TCACTTCAAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTCCATTT
    AAAGTTCCTG ATGAAGATGC CAGTCTGATC CCTCCTGAAA TGGATAATGA GTGTGTAGCA
    CAGACATGGT TTCGCTTCTT ACATATGTTA AGTAATCCCG TGGATTTGAG TAACCCGGCC
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTAA ATGTGAGCGG AATGCCACAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTAGTGGA TGCATTCTTA GGCATTTCTA GACCCCGATC AGACAGTGCT
    CCTCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGCA CTGCTGTCAG TACCACCCCA
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC GAAAGTTCAG CACCAGACAT CCTCCACCTC TCCTCTGTCA
    AGTCCCAATC AGACTAGTTC TGAACCCCGG CCACTGCCTG CCCCTCGAAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCAT CTTTTATCCA AATTCTTCTT
    TCTTATAAAT CTTCCATTAC AACACAAGCT AGCATGGAGT TTCGACGGAA AGGGTCACAA
    ATGTCCACAG ACACCATGGT TTCCAATCCT ATGTTTGATG CAAGTGAATT TCCTGATAAC
    TATGAAGCAG GAAGAGCTGA GGCTTGTGGG ACACTGTGTA GGATTTTTTG TAGCAAGAAG
    ACTGGAGAAG AGATTCTGCC AGCTTATTTA TCCAGATTTT ACATGCTTTT AATTCAAGGT
    TTGCAGATAA ATGATTATGT GTGCCATCCT GTCTTGGCCA GCGTTATTCT AAACTCCCCT
    CCTTTGTTCT GCTGTGACTT GAAAGGGATT GATGTTGTGG TTCCTTACTT TATTTCAGCT
    CTTGAAACCA TTTTGCCTGA CAGAGAACTC TCAAAATTCA AAAGCTATGT AAATCCAACA
    GAATTGAGAA GATCTTCCAT TAATATCCTC CTTTCTTTAT TGCCCCTCCC TCATCATTTT
    GGCACAGTCA GATCTGAGGT GGTCCTGGAA GGGAAGTTTA GTAATGATGA CAGCTCTTCA
    TATGATAAAC CAATAACTTT TTTGTCCCTG AAGTTGAGAC TTGTGAATAT ACTAATAGGT
    GCCTTGCAAA CGGAAACGGA CCCCAACAAC ACTCAAATGA TACTAGGGGC AATGTTAAAT
    ATTGTTCAAG ATTCAGCACT TTTAGAAGCC ATTGGTTGCC AAATGGAGAT GGGTGGTGGA
    GAAAATAACC TGAAGAGTCA TAGTCGTACT AATAGTGGTA TTAGTTCAGC AAGTGGTGGA
    AGCACAGAAC CCACAACTCC TGATAGTGAA AGACCTGCTC AAGCTCTCCT AAGGGATTAT
    GATTCAGCTG CTGGGCTCCT GATTCGCAGC ATTCATCTTG TCACCCAAAG ACTCAACTCT
    CAGTGGCGGC AAGACATGAG CATATCACTG GCAGCGCTGG AGCTCCTCTC TGGCCTGGCA
    AAGGTGAAGG TGATGGTTGA CTCGGGAGAC CGGAAACGAG CCATCAGTTC TGTGTGCAGC
    TACATCGTGT ACCAATGTAG TCGGCCAGCT CCTCTTCACT CCCGCGATCT GCACTCCATG
    ATAGTAGCAG CTTTTCAGTG TCTCTGTGTC TGGCTGACAG AGCATCCTGA TATGCTTGAT
    GAAAAGGACT GTCTTAAGGA AGTATTGGAG ATTGTGGAAC TGGGTATTTC AGGAAGTAAG
    TCCAAGAACA GTGAGCAAGA GGTCAAGTAC AAAGGAGATA AGGAGCCAAA CCCTGCATCT
    ATGAGGGTAA AGGATGCTGC TGAAGCCACT CTAACATGTA TTATGCAGTT GCTCGGCGCG
    TTTCCTTCAC CCAGTGGTCC TGCCTCTCCT TGTAGTCTGG TGAATGAGAC CACTTTGATT
    AAATACTCAA GGCTGCCAAC CATAAACAAG CATAGTTTCC GGTACTTTGT CTTGGATAAC
    AGTGTCATCC TGGCAATGCT GGAGCAACCT CTTGGAAATG AACAGAATGA TTTTTTTCCC
    TCTGTCACTG TGCTGGTTCG AGGAATGTCT GGAAGACTTG CTTGGGCACA ACAACTTTGC
    CTTTTACCCA GAGGAGCAAA AGCAAATCAG AAGCTTTTTG TACCTGAACC TCGTCCAATT
    CCTAAAAATG ATGTTGGATT TAAATATACT GTCAAACATC GACCATTTCC TGAAGAGGTG
    GATAAGATTC CTTTTGTGAA AGCAGATCTG AGCATTCCAG ATTTGCATGA AATTGTCACT
    GAAGAATTAG AAGAGAGACA TGAAAAATTA AGGAATGGCA TGGCCCAGCA GATTGCTTAT
    GAAATACACC TTGAACAACA AAGTGAGGAA GAATTGCAGA AGAGAAGTTT TCCTGATCCA
    ATTACGGATT GTAAGCCTCC ACCTCCTGCC CAAGAATTCC AAACAGCACG CCTCTTCCTT
    TCACACTTTG GATTTTTGTC TTTAGAAGCA TTGAAGGAAC CTGCAAATAG TCGCCTACCT
    CCTCACCTTA TTGCACTTGA CTCCACGATA CCTGGATTTT TTGATGACAT TGGATATCTG
    GATCTCTTGC CGTGTCGTCC CTTTGACACA GTTTTTATTT TCTATATGAA ACCAGGTCAG
    AAAACAAACC AGGAGATTTT AAAGAATGTG GAATCTTCCA GAAATGTTCA GCCACATTTC
    CTAGAATTTT TGCTCTCCCT TGGCTGGTCA GTAGATGTGG GCAGACACCC AGGTTGGACT
    GGGCATGTTT CTACCAGTTG GTCTATTAAC AGTTGTGATG ATGGTGAAGG ACCTGAACAA
    GATGAAGTGA TTTCCTCTGA AGACATTGGG GCTAGCATTT TCAATGGACA GAAGAAGGTG
    CTGTATTATG CTGATGCCCT TACGGAAATA GCTTTTGTAG TTCCTTCTCC TGTGGAGTCT
    TTAACTGATT CATTAGAAAG TAACATCTCG GACCAAGATA GTGATTCAAA TATGGATCTT
    ATGCCTGGAA TTCTGAAACA GCCATCCCTG ACACTTGAGC TTTTCCCTAA TCACACAGAC
    AATCTTAATT CCTCACAGCG GCTTAGTCCC AGTTCCAGAA TGAAGAAGCT GCCTCAGGGT
    CGCCCTGTGC CTCCCCTTGG ACCTGAGACA AGAGTTTCTG TAGTCTGGGT GGAACGCTAT
    GATGATATAG AGAACTTTCC CCTCTCAGAC CTGCTGACAG AGATCAGTAC TGGTGTGGAA
    ACTACTGCAA ACAGCAGCAC TTCTCTGAGA TCTACCACTC TTGAAAAAGA AGTTCCTGTC
    ATCTTTATCC ACCCTTTAAA CACTGGATTA TTCCGGATAA AAATTCAAGG AGCCACTGGA
    AAATTTAACA TGGTCATCCC GCTCGTGGAT GGGATGATTG TCAGCAGACG AGCACTTGGT
    TTTCTGGTGA GGCAGACTGT AATTAATATT TGTAGAAGAA AGAGACTGGA GAGTGACTCC
    TACAGTCCAC CACATGTCCG CCGGAAACAG AAAATCACCG ACATTGTCAA CAAGTACCGG
    AACAAGCAGT TGGAGCCAGA GTTTTATACT TCACTTTTCC AGGAGGTTGG ACTCAAGAAC
    TGCTCTTCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022265152.1
    CDS370..4881
    Translation

    Target ORF information:

    RefSeq Version XM_022409444.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022409444.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATCCAGA ATGATCAGGG CCACACGAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGG CGAGAGGTGG CAAATGCAGT GGTCCATCCC
    CTTGGGCAGG CCTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAGTTTGTT AAAAACTGAC
    AAAGAAGTGA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGTGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTATTGCCG
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC TATATGTTCA AAGTATACTA
    AAACACCTGC AAAATCTTTT TGTACCAAGA CAGGAACAGG GCTCCAGTCA GATCCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTAGCCCGTG AGTCATCCAT CATGGCACGA
    GAAACGTGGG AAGTCTTACT ATTGTTTCTT CTGCAGATTA ATGACATACT TCTAGCCCCG
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTTTGGT TACTAGCTTG TACTCGGTGC TTTCCAACAC CTCCTTATTG GAAAACCGCC
    AAGGAGATGG TGGCAAACTG GAGGCACCAC CCGGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ACCTGTGCCC TCACTTCAAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTCCATTT
    AAAGTTCCTG ATGAAGATGC CAGTCTGATC CCTCCTGAAA TGGATAATGA GTGTGTAGCA
    CAGACATGGT TTCGCTTCTT ACATATGTTA AGTAATCCCG TGGATTTGAG TAACCCGGCC
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTAA ATGTGAGCGG AATGCCACAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTAGTGGA TGCATTCTTA GGCATTTCTA GACCCCGATC AGACAGTGCT
    CCTCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGCA CTGCTGTCAG TACCACCCCA
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC GAAAGTTCAG CACCAGACAT CCTCCACCTC TCCTCTGTCA
    AGTCCCAATC AGACTAGTTC TGAACCCCGG CCACTGCCTG CCCCTCGAAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCAT CTTTTATCCA AATTCTTCTT
    TCTTATAAAT CTTCCATTAC AACACAAGCT AGCATGGAGT TTCGACGGAA AGGGTCACAA
    ATGTCCACAG ACACCATGGT TTCCAATCCT ATGTTTGATG CAAGTGAATT TCCTGATAAC
    TATGAAGCAG GAAGAGCTGA GGCTTGTGGG ACACTGTGTA GGATTTTTTG TAGCAAGAAG
    ACTGGAGAAG AGATTCTGCC AGCTTATTTA TCCAGATTTT ACATGCTTTT AATTCAAGGT
    TTGCAGATAA ATGATTATGT GTGCCATCCT GTCTTGGCCA GCGTTATTCT AAACTCCCCT
    CCTTTGTTCT GCTGTGACTT GAAAGGGATT GATGTTGTGG TTCCTTACTT TATTTCAGCT
    CTTGAAACCA TTTTGCCTGA CAGAGAACTC TCAAAATTCA AAAGCTATGT AAATCCAACA
    GAATTGAGAA GATCTTCCAT TAATATCCTC CTTTCTTTAT TGCCCCTCCC TCATCATTTT
    GGCACAGTCA GATCTGAGGT GGTCCTGGAA GGGAAGTTTA GTAATGATGA CAGCTCTTCA
    TATGATAAAC CAATAACTTT TTTGTCCCTG AAGTTGAGAC TTGTGAATAT ACTAATAGGT
    GCCTTGCAAA CGGAAACGGA CCCCAACAAC ACTCAAATGA TACTAGGGGC AATGTTAAAT
    ATTGTTCAAG ATTCAGCACT TTTAGAAGCC ATTGGTTGCC AAATGGAGAT GGGTGGTGGA
    GAAAATAACC TGAAGAGTCA TAGTCGTACT AATAGTGGTA TTAGTTCAGC AAGTGGTGGA
    AGCACAGAAC CCACAACTCC TGATAGTGAA AGACCTGCTC AAGCTCTCCT AAGGGATTAT
    GATTCAGCTG CTGGGCTCCT GATTCGCAGC ATTCATCTTG TCACCCAAAG ACTCAACTCT
    CAGTGGCGGC AAGACATGAG CATATCACTG GCAGCGCTGG AGCTCCTCTC TGGCCTGGCA
    AAGGTGAAGG TGATGGTTGA CTCGGGAGAC CGGAAACGAG CCATCAGTTC TGTGTGCAGC
    TACATCGTGT ACCAATGTAG TCGGCCAGCT CCTCTTCACT CCCGCGATCT GCACTCCATG
    ATAGTAGCAG CTTTTCAGTG TCTCTGTGTC TGGCTGACAG AGCATCCTGA TATGCTTGAT
    GAAAAGGACT GTCTTAAGGA AGTATTGGAG ATTGTGGAAC TGGGTATTTC AGGAAGTAAG
    TCCAAGAACA GTGAGCAAGA GGTCAAGTAC AAAGGAGATA AGGAGCCAAA CCCTGCATCT
    ATGAGGGTAA AGGATGCTGC TGAAGCCACT CTAACATGTA TTATGCAGTT GCTCGGCGCG
    TTTCCTTCAC CCAGTGGTCC TGCCTCTCCT TGTAGTCTGG TGAATGAGAC CACTTTGATT
    AAATACTCAA GGCTGCCAAC CATAAACAAG CATAGTTTCC GGTACTTTGT CTTGGATAAC
    AGTGTCATCC TGGCAATGCT GGAGCAACCT CTTGGAAATG AACAGAATGA TTTTTTTCCC
    TCTGTCACTG TGCTGGTTCG AGGAATGTCT GGAAGACTTG CTTGGGCACA ACAACTTTGC
    CTTTTACCCA GAGGAGCAAA AGCAAATCAG AAGCTTTTTG TACCTGAACC TCGTCCAATT
    CCTAAAAATG ATGTTGGATT TAAATATACT GTCAAACATC GACCATTTCC TGAAGAGGTG
    GATAAGATTC CTTTTGTGAA AGCAGATCTG AGCATTCCAG ATTTGCATGA AATTGTCACT
    GAAGAATTAG AAGAGAGACA TGAAAAATTA AGGAATGGCA TGGCCCAGCA GATTGCTTAT
    GAAATACACC TTGAACAACA AAGTGAGGAA GAATTGCAGA AGAGAAGTTT TCCTGATCCA
    ATTACGGATT GTAAGCCTCC ACCTCCTGCC CAAGAATTCC AAACAGCACG CCTCTTCCTT
    TCACACTTTG GATTTTTGTC TTTAGAAGCA TTGAAGGAAC CTGCAAATAG TCGCCTACCT
    CCTCACCTTA TTGCACTTGA CTCCACGATA CCTGGATTTT TTGATGACAT TGGATATCTG
    GATCTCTTGC CGTGTCGTCC CTTTGACACA GTTTTTATTT TCTATATGAA ACCAGGTCAG
    AAAACAAACC AGGAGATTTT AAAGAATGTG GAATCTTCCA GAAATGTTCA GCCACATTTC
    CTAGAATTTT TGCTCTCCCT TGGCTGGTCA GTAGATGTGG GCAGACACCC AGGTTGGACT
    GGGCATGTTT CTACCAGTTG GTCTATTAAC AGTTGTGATG ATGGTGAAGG ACCTGAACAA
    GATGAAGTGA TTTCCTCTGA AGACATTGGG GCTAGCATTT TCAATGGACA GAAGAAGGTG
    CTGTATTATG CTGATGCCCT TACGGAAATA GCTTTTGTAG TTCCTTCTCC TGTGGAGTCT
    TTAACTGATT CATTAGAAAG TAACATCTCG GACCAAGATA GTGATTCAAA TATGGATCTT
    ATGCCTGGAA TTCTGAAACA GCCATCCCTG ACACTTGAGC TTTTCCCTAA TCACACAGAC
    AATCTTAATT CCTCACAGCG GCTTAGTCCC AGTTCCAGAA TGAAGAAGCT GCCTCAGGGT
    CGCCCTGTGC CTCCCCTTGG ACCTGAGACA AGAGTTTCTG TAGTCTGGGT GGAACGCTAT
    GATGATATAG AGAACTTTCC CCTCTCAGAC CTGCTGACAG AGATCAGTAC TGGTGTGGAA
    ACTACTGCAA ACAGCAGCAC TTCTCTGAGA TCTACCACTC TTGAAAAAGA AGTTCCTGTC
    ATCTTTATCC ACCCTTTAAA CACTGGATTA TTCCGGATAA AAATTCAAGG AGCCACTGGA
    AAATTTAACA TGGTCATCCC GCTCGTGGAT GGGATGATTG TCAGCAGACG AGCACTTGGT
    TTTCTGGTGA GGCAGACTGT AATTAATATT TGTAGAAGAA AGAGACTGGA GAGTGACTCC
    TACAGTCCAC CACATGTCCG CCGGAAACAG AAAATCACCG ACATTGTCAA CAAGTACCGG
    AACAAGCAGT TGGAGCCAGA GTTTTATACT TCACTTTTCC AGGAGGTTGG ACTCAAGAAC
    TGCTCTTCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe24891
    Clone ID Related Accession (Same CDS sequence) XM_022409444.1 , XM_005635053.2
    Accession Version XM_005635053.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4512bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ral GTPase-activating protein subunit beta isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726101.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005635053.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATCCAGA ATGATCAGGG CCACACGAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGG CGAGAGGTGG CAAATGCAGT GGTCCATCCC
    CTTGGGCAGG CCTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAGTTTGTT AAAAACTGAC
    AAAGAAGTGA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGTGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTATTGCCG
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC TATATGTTCA AAGTATACTA
    AAACACCTGC AAAATCTTTT TGTACCAAGA CAGGAACAGG GCTCCAGTCA GATCCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTAGCCCGTG AGTCATCCAT CATGGCACGA
    GAAACGTGGG AAGTCTTACT ATTGTTTCTT CTGCAGATTA ATGACATACT TCTAGCCCCG
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTTTGGT TACTAGCTTG TACTCGGTGC TTTCCAACAC CTCCTTATTG GAAAACCGCC
    AAGGAGATGG TGGCAAACTG GAGGCACCAC CCGGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ACCTGTGCCC TCACTTCAAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTCCATTT
    AAAGTTCCTG ATGAAGATGC CAGTCTGATC CCTCCTGAAA TGGATAATGA GTGTGTAGCA
    CAGACATGGT TTCGCTTCTT ACATATGTTA AGTAATCCCG TGGATTTGAG TAACCCGGCC
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTAA ATGTGAGCGG AATGCCACAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTAGTGGA TGCATTCTTA GGCATTTCTA GACCCCGATC AGACAGTGCT
    CCTCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGCA CTGCTGTCAG TACCACCCCA
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC GAAAGTTCAG CACCAGACAT CCTCCACCTC TCCTCTGTCA
    AGTCCCAATC AGACTAGTTC TGAACCCCGG CCACTGCCTG CCCCTCGAAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCAT CTTTTATCCA AATTCTTCTT
    TCTTATAAAT CTTCCATTAC AACACAAGCT AGCATGGAGT TTCGACGGAA AGGGTCACAA
    ATGTCCACAG ACACCATGGT TTCCAATCCT ATGTTTGATG CAAGTGAATT TCCTGATAAC
    TATGAAGCAG GAAGAGCTGA GGCTTGTGGG ACACTGTGTA GGATTTTTTG TAGCAAGAAG
    ACTGGAGAAG AGATTCTGCC AGCTTATTTA TCCAGATTTT ACATGCTTTT AATTCAAGGT
    TTGCAGATAA ATGATTATGT GTGCCATCCT GTCTTGGCCA GCGTTATTCT AAACTCCCCT
    CCTTTGTTCT GCTGTGACTT GAAAGGGATT GATGTTGTGG TTCCTTACTT TATTTCAGCT
    CTTGAAACCA TTTTGCCTGA CAGAGAACTC TCAAAATTCA AAAGCTATGT AAATCCAACA
    GAATTGAGAA GATCTTCCAT TAATATCCTC CTTTCTTTAT TGCCCCTCCC TCATCATTTT
    GGCACAGTCA GATCTGAGGT GGTCCTGGAA GGGAAGTTTA GTAATGATGA CAGCTCTTCA
    TATGATAAAC CAATAACTTT TTTGTCCCTG AAGTTGAGAC TTGTGAATAT ACTAATAGGT
    GCCTTGCAAA CGGAAACGGA CCCCAACAAC ACTCAAATGA TACTAGGGGC AATGTTAAAT
    ATTGTTCAAG ATTCAGCACT TTTAGAAGCC ATTGGTTGCC AAATGGAGAT GGGTGGTGGA
    GAAAATAACC TGAAGAGTCA TAGTCGTACT AATAGTGGTA TTAGTTCAGC AAGTGGTGGA
    AGCACAGAAC CCACAACTCC TGATAGTGAA AGACCTGCTC AAGCTCTCCT AAGGGATTAT
    GATTCAGCTG CTGGGCTCCT GATTCGCAGC ATTCATCTTG TCACCCAAAG ACTCAACTCT
    CAGTGGCGGC AAGACATGAG CATATCACTG GCAGCGCTGG AGCTCCTCTC TGGCCTGGCA
    AAGGTGAAGG TGATGGTTGA CTCGGGAGAC CGGAAACGAG CCATCAGTTC TGTGTGCAGC
    TACATCGTGT ACCAATGTAG TCGGCCAGCT CCTCTTCACT CCCGCGATCT GCACTCCATG
    ATAGTAGCAG CTTTTCAGTG TCTCTGTGTC TGGCTGACAG AGCATCCTGA TATGCTTGAT
    GAAAAGGACT GTCTTAAGGA AGTATTGGAG ATTGTGGAAC TGGGTATTTC AGGAAGTAAG
    TCCAAGAACA GTGAGCAAGA GGTCAAGTAC AAAGGAGATA AGGAGCCAAA CCCTGCATCT
    ATGAGGGTAA AGGATGCTGC TGAAGCCACT CTAACATGTA TTATGCAGTT GCTCGGCGCG
    TTTCCTTCAC CCAGTGGTCC TGCCTCTCCT TGTAGTCTGG TGAATGAGAC CACTTTGATT
    AAATACTCAA GGCTGCCAAC CATAAACAAG CATAGTTTCC GGTACTTTGT CTTGGATAAC
    AGTGTCATCC TGGCAATGCT GGAGCAACCT CTTGGAAATG AACAGAATGA TTTTTTTCCC
    TCTGTCACTG TGCTGGTTCG AGGAATGTCT GGAAGACTTG CTTGGGCACA ACAACTTTGC
    CTTTTACCCA GAGGAGCAAA AGCAAATCAG AAGCTTTTTG TACCTGAACC TCGTCCAATT
    CCTAAAAATG ATGTTGGATT TAAATATACT GTCAAACATC GACCATTTCC TGAAGAGGTG
    GATAAGATTC CTTTTGTGAA AGCAGATCTG AGCATTCCAG ATTTGCATGA AATTGTCACT
    GAAGAATTAG AAGAGAGACA TGAAAAATTA AGGAATGGCA TGGCCCAGCA GATTGCTTAT
    GAAATACACC TTGAACAACA AAGTGAGGAA GAATTGCAGA AGAGAAGTTT TCCTGATCCA
    ATTACGGATT GTAAGCCTCC ACCTCCTGCC CAAGAATTCC AAACAGCACG CCTCTTCCTT
    TCACACTTTG GATTTTTGTC TTTAGAAGCA TTGAAGGAAC CTGCAAATAG TCGCCTACCT
    CCTCACCTTA TTGCACTTGA CTCCACGATA CCTGGATTTT TTGATGACAT TGGATATCTG
    GATCTCTTGC CGTGTCGTCC CTTTGACACA GTTTTTATTT TCTATATGAA ACCAGGTCAG
    AAAACAAACC AGGAGATTTT AAAGAATGTG GAATCTTCCA GAAATGTTCA GCCACATTTC
    CTAGAATTTT TGCTCTCCCT TGGCTGGTCA GTAGATGTGG GCAGACACCC AGGTTGGACT
    GGGCATGTTT CTACCAGTTG GTCTATTAAC AGTTGTGATG ATGGTGAAGG ACCTGAACAA
    GATGAAGTGA TTTCCTCTGA AGACATTGGG GCTAGCATTT TCAATGGACA GAAGAAGGTG
    CTGTATTATG CTGATGCCCT TACGGAAATA GCTTTTGTAG TTCCTTCTCC TGTGGAGTCT
    TTAACTGATT CATTAGAAAG TAACATCTCG GACCAAGATA GTGATTCAAA TATGGATCTT
    ATGCCTGGAA TTCTGAAACA GCCATCCCTG ACACTTGAGC TTTTCCCTAA TCACACAGAC
    AATCTTAATT CCTCACAGCG GCTTAGTCCC AGTTCCAGAA TGAAGAAGCT GCCTCAGGGT
    CGCCCTGTGC CTCCCCTTGG ACCTGAGACA AGAGTTTCTG TAGTCTGGGT GGAACGCTAT
    GATGATATAG AGAACTTTCC CCTCTCAGAC CTGCTGACAG AGATCAGTAC TGGTGTGGAA
    ACTACTGCAA ACAGCAGCAC TTCTCTGAGA TCTACCACTC TTGAAAAAGA AGTTCCTGTC
    ATCTTTATCC ACCCTTTAAA CACTGGATTA TTCCGGATAA AAATTCAAGG AGCCACTGGA
    AAATTTAACA TGGTCATCCC GCTCGTGGAT GGGATGATTG TCAGCAGACG AGCACTTGGT
    TTTCTGGTGA GGCAGACTGT AATTAATATT TGTAGAAGAA AGAGACTGGA GAGTGACTCC
    TACAGTCCAC CACATGTCCG CCGGAAACAG AAAATCACCG ACATTGTCAA CAAGTACCGG
    AACAAGCAGT TGGAGCCAGA GTTTTATACT TCACTTTTCC AGGAGGTTGG ACTCAAGAAC
    TGCTCTTCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005635110.1
    CDS333..4844
    Translation

    Target ORF information:

    RefSeq Version XM_005635053.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Ral GTPase activating protein, beta subunit (non-catalytic) (RALGAPB), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005635053.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATCCAGA ATGATCAGGG CCACACGAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGG CGAGAGGTGG CAAATGCAGT GGTCCATCCC
    CTTGGGCAGG CCTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAGTTTGTT AAAAACTGAC
    AAAGAAGTGA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGTGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTATTGCCG
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC TATATGTTCA AAGTATACTA
    AAACACCTGC AAAATCTTTT TGTACCAAGA CAGGAACAGG GCTCCAGTCA GATCCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTAGCCCGTG AGTCATCCAT CATGGCACGA
    GAAACGTGGG AAGTCTTACT ATTGTTTCTT CTGCAGATTA ATGACATACT TCTAGCCCCG
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTTTGGT TACTAGCTTG TACTCGGTGC TTTCCAACAC CTCCTTATTG GAAAACCGCC
    AAGGAGATGG TGGCAAACTG GAGGCACCAC CCGGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ACCTGTGCCC TCACTTCAAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTCCATTT
    AAAGTTCCTG ATGAAGATGC CAGTCTGATC CCTCCTGAAA TGGATAATGA GTGTGTAGCA
    CAGACATGGT TTCGCTTCTT ACATATGTTA AGTAATCCCG TGGATTTGAG TAACCCGGCC
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTAA ATGTGAGCGG AATGCCACAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTAGTGGA TGCATTCTTA GGCATTTCTA GACCCCGATC AGACAGTGCT
    CCTCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGCA CTGCTGTCAG TACCACCCCA
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC GAAAGTTCAG CACCAGACAT CCTCCACCTC TCCTCTGTCA
    AGTCCCAATC AGACTAGTTC TGAACCCCGG CCACTGCCTG CCCCTCGAAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCAT CTTTTATCCA AATTCTTCTT
    TCTTATAAAT CTTCCATTAC AACACAAGCT AGCATGGAGT TTCGACGGAA AGGGTCACAA
    ATGTCCACAG ACACCATGGT TTCCAATCCT ATGTTTGATG CAAGTGAATT TCCTGATAAC
    TATGAAGCAG GAAGAGCTGA GGCTTGTGGG ACACTGTGTA GGATTTTTTG TAGCAAGAAG
    ACTGGAGAAG AGATTCTGCC AGCTTATTTA TCCAGATTTT ACATGCTTTT AATTCAAGGT
    TTGCAGATAA ATGATTATGT GTGCCATCCT GTCTTGGCCA GCGTTATTCT AAACTCCCCT
    CCTTTGTTCT GCTGTGACTT GAAAGGGATT GATGTTGTGG TTCCTTACTT TATTTCAGCT
    CTTGAAACCA TTTTGCCTGA CAGAGAACTC TCAAAATTCA AAAGCTATGT AAATCCAACA
    GAATTGAGAA GATCTTCCAT TAATATCCTC CTTTCTTTAT TGCCCCTCCC TCATCATTTT
    GGCACAGTCA GATCTGAGGT GGTCCTGGAA GGGAAGTTTA GTAATGATGA CAGCTCTTCA
    TATGATAAAC CAATAACTTT TTTGTCCCTG AAGTTGAGAC TTGTGAATAT ACTAATAGGT
    GCCTTGCAAA CGGAAACGGA CCCCAACAAC ACTCAAATGA TACTAGGGGC AATGTTAAAT
    ATTGTTCAAG ATTCAGCACT TTTAGAAGCC ATTGGTTGCC AAATGGAGAT GGGTGGTGGA
    GAAAATAACC TGAAGAGTCA TAGTCGTACT AATAGTGGTA TTAGTTCAGC AAGTGGTGGA
    AGCACAGAAC CCACAACTCC TGATAGTGAA AGACCTGCTC AAGCTCTCCT AAGGGATTAT
    GATTCAGCTG CTGGGCTCCT GATTCGCAGC ATTCATCTTG TCACCCAAAG ACTCAACTCT
    CAGTGGCGGC AAGACATGAG CATATCACTG GCAGCGCTGG AGCTCCTCTC TGGCCTGGCA
    AAGGTGAAGG TGATGGTTGA CTCGGGAGAC CGGAAACGAG CCATCAGTTC TGTGTGCAGC
    TACATCGTGT ACCAATGTAG TCGGCCAGCT CCTCTTCACT CCCGCGATCT GCACTCCATG
    ATAGTAGCAG CTTTTCAGTG TCTCTGTGTC TGGCTGACAG AGCATCCTGA TATGCTTGAT
    GAAAAGGACT GTCTTAAGGA AGTATTGGAG ATTGTGGAAC TGGGTATTTC AGGAAGTAAG
    TCCAAGAACA GTGAGCAAGA GGTCAAGTAC AAAGGAGATA AGGAGCCAAA CCCTGCATCT
    ATGAGGGTAA AGGATGCTGC TGAAGCCACT CTAACATGTA TTATGCAGTT GCTCGGCGCG
    TTTCCTTCAC CCAGTGGTCC TGCCTCTCCT TGTAGTCTGG TGAATGAGAC CACTTTGATT
    AAATACTCAA GGCTGCCAAC CATAAACAAG CATAGTTTCC GGTACTTTGT CTTGGATAAC
    AGTGTCATCC TGGCAATGCT GGAGCAACCT CTTGGAAATG AACAGAATGA TTTTTTTCCC
    TCTGTCACTG TGCTGGTTCG AGGAATGTCT GGAAGACTTG CTTGGGCACA ACAACTTTGC
    CTTTTACCCA GAGGAGCAAA AGCAAATCAG AAGCTTTTTG TACCTGAACC TCGTCCAATT
    CCTAAAAATG ATGTTGGATT TAAATATACT GTCAAACATC GACCATTTCC TGAAGAGGTG
    GATAAGATTC CTTTTGTGAA AGCAGATCTG AGCATTCCAG ATTTGCATGA AATTGTCACT
    GAAGAATTAG AAGAGAGACA TGAAAAATTA AGGAATGGCA TGGCCCAGCA GATTGCTTAT
    GAAATACACC TTGAACAACA AAGTGAGGAA GAATTGCAGA AGAGAAGTTT TCCTGATCCA
    ATTACGGATT GTAAGCCTCC ACCTCCTGCC CAAGAATTCC AAACAGCACG CCTCTTCCTT
    TCACACTTTG GATTTTTGTC TTTAGAAGCA TTGAAGGAAC CTGCAAATAG TCGCCTACCT
    CCTCACCTTA TTGCACTTGA CTCCACGATA CCTGGATTTT TTGATGACAT TGGATATCTG
    GATCTCTTGC CGTGTCGTCC CTTTGACACA GTTTTTATTT TCTATATGAA ACCAGGTCAG
    AAAACAAACC AGGAGATTTT AAAGAATGTG GAATCTTCCA GAAATGTTCA GCCACATTTC
    CTAGAATTTT TGCTCTCCCT TGGCTGGTCA GTAGATGTGG GCAGACACCC AGGTTGGACT
    GGGCATGTTT CTACCAGTTG GTCTATTAAC AGTTGTGATG ATGGTGAAGG ACCTGAACAA
    GATGAAGTGA TTTCCTCTGA AGACATTGGG GCTAGCATTT TCAATGGACA GAAGAAGGTG
    CTGTATTATG CTGATGCCCT TACGGAAATA GCTTTTGTAG TTCCTTCTCC TGTGGAGTCT
    TTAACTGATT CATTAGAAAG TAACATCTCG GACCAAGATA GTGATTCAAA TATGGATCTT
    ATGCCTGGAA TTCTGAAACA GCCATCCCTG ACACTTGAGC TTTTCCCTAA TCACACAGAC
    AATCTTAATT CCTCACAGCG GCTTAGTCCC AGTTCCAGAA TGAAGAAGCT GCCTCAGGGT
    CGCCCTGTGC CTCCCCTTGG ACCTGAGACA AGAGTTTCTG TAGTCTGGGT GGAACGCTAT
    GATGATATAG AGAACTTTCC CCTCTCAGAC CTGCTGACAG AGATCAGTAC TGGTGTGGAA
    ACTACTGCAA ACAGCAGCAC TTCTCTGAGA TCTACCACTC TTGAAAAAGA AGTTCCTGTC
    ATCTTTATCC ACCCTTTAAA CACTGGATTA TTCCGGATAA AAATTCAAGG AGCCACTGGA
    AAATTTAACA TGGTCATCCC GCTCGTGGAT GGGATGATTG TCAGCAGACG AGCACTTGGT
    TTTCTGGTGA GGCAGACTGT AATTAATATT TGTAGAAGAA AGAGACTGGA GAGTGACTCC
    TACAGTCCAC CACATGTCCG CCGGAAACAG AAAATCACCG ACATTGTCAA CAAGTACCGG
    AACAAGCAGT TGGAGCCAGA GTTTTATACT TCACTTTTCC AGGAGGTTGG ACTCAAGAAC
    TGCTCTTCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe24892
    Clone ID Related Accession (Same CDS sequence) XM_005635054.2 , XM_022409445.1
    Accession Version XM_005635054.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4497bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ral GTPase-activating protein subunit beta isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726101.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005635054.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATCCAGA ATGATCAGGG CCACACGAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGG CGAGAGGTGG CAAATGCAGT GGTCCATCCC
    CTTGGGCAGG CCTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAGTTTGTT AAAAACTGAC
    AAAGAAGTGA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGTGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTATTGCCG
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC TATATGTTCA AAGTATACTA
    AAACACCTGC AAAATCTTTT TGTACCAAGA CAGGAACAGG GCTCCAGTCA GATCCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTAGCCCGTG AGTCATCCAT CATGGCACGA
    GAAACGTGGG AAGTCTTACT ATTGTTTCTT CTGCAGATTA ATGACATACT TCTAGCCCCG
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTTTGGT TACTAGCTTG TACTCGGTGC TTTCCAACAC CTCCTTATTG GAAAACCGCC
    AAGGAGATGG TGGCAAACTG GAGGCACCAC CCGGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ACCTGTGCCC TCACTTCAAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTCCATTT
    AAAGTTCCTG ATGAAGATGC CAGTCTGATC CCTCCTGAAA TGGATAATGA GTGTGTAGCA
    CAGACATGGT TTCGCTTCTT ACATATGTTA AGTAATCCCG TGGATTTGAG TAACCCGGCC
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTAA ATGTGAGCGG AATGCCACAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTAGTGGA TGCATTCTTA GGCATTTCTA GACCCCGATC AGACAGTGCT
    CCTCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGCA CTGCTGTCAG TACCACCCCA
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC GAAAGTTCAG CACCAGACAT CCTCCACCTC TCCTCTGTCA
    AGTCCCAATC AGACTAGTTC TGAACCCCGG CCACTGCCTG CCCCTCGAAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCCA TTACAACACA AGCTAGCATG
    GAGTTTCGAC GGAAAGGGTC ACAAATGTCC ACAGACACCA TGGTTTCCAA TCCTATGTTT
    GATGCAAGTG AATTTCCTGA TAACTATGAA GCAGGAAGAG CTGAGGCTTG TGGGACACTG
    TGTAGGATTT TTTGTAGCAA GAAGACTGGA GAAGAGATTC TGCCAGCTTA TTTATCCAGA
    TTTTACATGC TTTTAATTCA AGGTTTGCAG ATAAATGATT ATGTGTGCCA TCCTGTCTTG
    GCCAGCGTTA TTCTAAACTC CCCTCCTTTG TTCTGCTGTG ACTTGAAAGG GATTGATGTT
    GTGGTTCCTT ACTTTATTTC AGCTCTTGAA ACCATTTTGC CTGACAGAGA ACTCTCAAAA
    TTCAAAAGCT ATGTAAATCC AACAGAATTG AGAAGATCTT CCATTAATAT CCTCCTTTCT
    TTATTGCCCC TCCCTCATCA TTTTGGCACA GTCAGATCTG AGGTGGTCCT GGAAGGGAAG
    TTTAGTAATG ATGACAGCTC TTCATATGAT AAACCAATAA CTTTTTTGTC CCTGAAGTTG
    AGACTTGTGA ATATACTAAT AGGTGCCTTG CAAACGGAAA CGGACCCCAA CAACACTCAA
    ATGATACTAG GGGCAATGTT AAATATTGTT CAAGATTCAG CACTTTTAGA AGCCATTGGT
    TGCCAAATGG AGATGGGTGG TGGAGAAAAT AACCTGAAGA GTCATAGTCG TACTAATAGT
    GGTATTAGTT CAGCAAGTGG TGGAAGCACA GAACCCACAA CTCCTGATAG TGAAAGACCT
    GCTCAAGCTC TCCTAAGGGA TTATGGATCG ACAGCTCTTA ATACAGATTC AGCTGCTGGG
    CTCCTGATTC GCAGCATTCA TCTTGTCACC CAAAGACTCA ACTCTCAGTG GCGGCAAGAC
    ATGAGCATAT CACTGGCAGC GCTGGAGCTC CTCTCTGGCC TGGCAAAGGT GAAGGTGATG
    GTTGACTCGG GAGACCGGAA ACGAGCCATC AGTTCTGTGT GCAGCTACAT CGTGTACCAA
    TGTAGTCGGC CAGCTCCTCT TCACTCCCGC GATCTGCACT CCATGATAGT AGCAGCTTTT
    CAGTGTCTCT GTGTCTGGCT GACAGAGCAT CCTGATATGC TTGATGAAAA GGACTGTCTT
    AAGGAAGTAT TGGAGATTGT GGAACTGGGT ATTTCAGGAA GTAAGTCCAA GAACAGTGAG
    CAAGAGGTCA AGTACAAAGG AGATAAGGAG CCAAACCCTG CATCTATGAG GGTAAAGGAT
    GCTGCTGAAG CCACTCTAAC ATGTATTATG CAGTTGCTCG GCGCGTTTCC TTCACCCAGT
    GGTCCTGCCT CTCCTTGTAG TCTGGTGAAT GAGACCACTT TGATTAAATA CTCAAGGCTG
    CCAACCATAA ACAAGCATAG TTTCCGGTAC TTTGTCTTGG ATAACAGTGT CATCCTGGCA
    ATGCTGGAGC AACCTCTTGG AAATGAACAG AATGATTTTT TTCCCTCTGT CACTGTGCTG
    GTTCGAGGAA TGTCTGGAAG ACTTGCTTGG GCACAACAAC TTTGCCTTTT ACCCAGAGGA
    GCAAAAGCAA ATCAGAAGCT TTTTGTACCT GAACCTCGTC CAATTCCTAA AAATGATGTT
    GGATTTAAAT ATACTGTCAA ACATCGACCA TTTCCTGAAG AGGTGGATAA GATTCCTTTT
    GTGAAAGCAG ATCTGAGCAT TCCAGATTTG CATGAAATTG TCACTGAAGA ATTAGAAGAG
    AGACATGAAA AATTAAGGAA TGGCATGGCC CAGCAGATTG CTTATGAAAT ACACCTTGAA
    CAACAAAGTG AGGAAGAATT GCAGAAGAGA AGTTTTCCTG ATCCAATTAC GGATTGTAAG
    CCTCCACCTC CTGCCCAAGA ATTCCAAACA GCACGCCTCT TCCTTTCACA CTTTGGATTT
    TTGTCTTTAG AAGCATTGAA GGAACCTGCA AATAGTCGCC TACCTCCTCA CCTTATTGCA
    CTTGACTCCA CGATACCTGG ATTTTTTGAT GACATTGGAT ATCTGGATCT CTTGCCGTGT
    CGTCCCTTTG ACACAGTTTT TATTTTCTAT ATGAAACCAG GTCAGAAAAC AAACCAGGAG
    ATTTTAAAGA ATGTGGAATC TTCCAGAAAT GTTCAGCCAC ATTTCCTAGA ATTTTTGCTC
    TCCCTTGGCT GGTCAGTAGA TGTGGGCAGA CACCCAGGTT GGACTGGGCA TGTTTCTACC
    AGTTGGTCTA TTAACAGTTG TGATGATGGT GAAGGACCTG AACAAGATGA AGTGATTTCC
    TCTGAAGACA TTGGGGCTAG CATTTTCAAT GGACAGAAGA AGGTGCTGTA TTATGCTGAT
    GCCCTTACGG AAATAGCTTT TGTAGTTCCT TCTCCTGTGG AGTCTTTAAC TGATTCATTA
    GAAAGTAACA TCTCGGACCA AGATAGTGAT TCAAATATGG ATCTTATGCC TGGAATTCTG
    AAACAGCCAT CCCTGACACT TGAGCTTTTC CCTAATCACA CAGACAATCT TAATTCCTCA
    CAGCGGCTTA GTCCCAGTTC CAGAATGAAG AAGCTGCCTC AGGGTCGCCC TGTGCCTCCC
    CTTGGACCTG AGACAAGAGT TTCTGTAGTC TGGGTGGAAC GCTATGATGA TATAGAGAAC
    TTTCCCCTCT CAGACCTGCT GACAGAGATC AGTACTGGTG TGGAAACTAC TGCAAACAGC
    AGCACTTCTC TGAGATCTAC CACTCTTGAA AAAGAAGTTC CTGTCATCTT TATCCACCCT
    TTAAACACTG GATTATTCCG GATAAAAATT CAAGGAGCCA CTGGAAAATT TAACATGGTC
    ATCCCGCTCG TGGATGGGAT GATTGTCAGC AGACGAGCAC TTGGTTTTCT GGTGAGGCAG
    ACTGTAATTA ATATTTGTAG AAGAAAGAGA CTGGAGAGTG ACTCCTACAG TCCACCACAT
    GTCCGCCGGA AACAGAAAAT CACCGACATT GTCAACAAGT ACCGGAACAA GCAGTTGGAG
    CCAGAGTTTT ATACTTCACT TTTCCAGGAG GTTGGACTCA AGAACTGCTC TTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005635111.1
    CDS333..4829
    Translation

    Target ORF information:

    RefSeq Version XM_005635054.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Ral GTPase activating protein, beta subunit (non-catalytic) (RALGAPB), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005635054.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATCCAGA ATGATCAGGG CCACACGAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGG CGAGAGGTGG CAAATGCAGT GGTCCATCCC
    CTTGGGCAGG CCTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAGTTTGTT AAAAACTGAC
    AAAGAAGTGA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGTGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTATTGCCG
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC TATATGTTCA AAGTATACTA
    AAACACCTGC AAAATCTTTT TGTACCAAGA CAGGAACAGG GCTCCAGTCA GATCCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTAGCCCGTG AGTCATCCAT CATGGCACGA
    GAAACGTGGG AAGTCTTACT ATTGTTTCTT CTGCAGATTA ATGACATACT TCTAGCCCCG
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTTTGGT TACTAGCTTG TACTCGGTGC TTTCCAACAC CTCCTTATTG GAAAACCGCC
    AAGGAGATGG TGGCAAACTG GAGGCACCAC CCGGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ACCTGTGCCC TCACTTCAAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTCCATTT
    AAAGTTCCTG ATGAAGATGC CAGTCTGATC CCTCCTGAAA TGGATAATGA GTGTGTAGCA
    CAGACATGGT TTCGCTTCTT ACATATGTTA AGTAATCCCG TGGATTTGAG TAACCCGGCC
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTAA ATGTGAGCGG AATGCCACAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTAGTGGA TGCATTCTTA GGCATTTCTA GACCCCGATC AGACAGTGCT
    CCTCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGCA CTGCTGTCAG TACCACCCCA
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC GAAAGTTCAG CACCAGACAT CCTCCACCTC TCCTCTGTCA
    AGTCCCAATC AGACTAGTTC TGAACCCCGG CCACTGCCTG CCCCTCGAAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCCA TTACAACACA AGCTAGCATG
    GAGTTTCGAC GGAAAGGGTC ACAAATGTCC ACAGACACCA TGGTTTCCAA TCCTATGTTT
    GATGCAAGTG AATTTCCTGA TAACTATGAA GCAGGAAGAG CTGAGGCTTG TGGGACACTG
    TGTAGGATTT TTTGTAGCAA GAAGACTGGA GAAGAGATTC TGCCAGCTTA TTTATCCAGA
    TTTTACATGC TTTTAATTCA AGGTTTGCAG ATAAATGATT ATGTGTGCCA TCCTGTCTTG
    GCCAGCGTTA TTCTAAACTC CCCTCCTTTG TTCTGCTGTG ACTTGAAAGG GATTGATGTT
    GTGGTTCCTT ACTTTATTTC AGCTCTTGAA ACCATTTTGC CTGACAGAGA ACTCTCAAAA
    TTCAAAAGCT ATGTAAATCC AACAGAATTG AGAAGATCTT CCATTAATAT CCTCCTTTCT
    TTATTGCCCC TCCCTCATCA TTTTGGCACA GTCAGATCTG AGGTGGTCCT GGAAGGGAAG
    TTTAGTAATG ATGACAGCTC TTCATATGAT AAACCAATAA CTTTTTTGTC CCTGAAGTTG
    AGACTTGTGA ATATACTAAT AGGTGCCTTG CAAACGGAAA CGGACCCCAA CAACACTCAA
    ATGATACTAG GGGCAATGTT AAATATTGTT CAAGATTCAG CACTTTTAGA AGCCATTGGT
    TGCCAAATGG AGATGGGTGG TGGAGAAAAT AACCTGAAGA GTCATAGTCG TACTAATAGT
    GGTATTAGTT CAGCAAGTGG TGGAAGCACA GAACCCACAA CTCCTGATAG TGAAAGACCT
    GCTCAAGCTC TCCTAAGGGA TTATGGATCG ACAGCTCTTA ATACAGATTC AGCTGCTGGG
    CTCCTGATTC GCAGCATTCA TCTTGTCACC CAAAGACTCA ACTCTCAGTG GCGGCAAGAC
    ATGAGCATAT CACTGGCAGC GCTGGAGCTC CTCTCTGGCC TGGCAAAGGT GAAGGTGATG
    GTTGACTCGG GAGACCGGAA ACGAGCCATC AGTTCTGTGT GCAGCTACAT CGTGTACCAA
    TGTAGTCGGC CAGCTCCTCT TCACTCCCGC GATCTGCACT CCATGATAGT AGCAGCTTTT
    CAGTGTCTCT GTGTCTGGCT GACAGAGCAT CCTGATATGC TTGATGAAAA GGACTGTCTT
    AAGGAAGTAT TGGAGATTGT GGAACTGGGT ATTTCAGGAA GTAAGTCCAA GAACAGTGAG
    CAAGAGGTCA AGTACAAAGG AGATAAGGAG CCAAACCCTG CATCTATGAG GGTAAAGGAT
    GCTGCTGAAG CCACTCTAAC ATGTATTATG CAGTTGCTCG GCGCGTTTCC TTCACCCAGT
    GGTCCTGCCT CTCCTTGTAG TCTGGTGAAT GAGACCACTT TGATTAAATA CTCAAGGCTG
    CCAACCATAA ACAAGCATAG TTTCCGGTAC TTTGTCTTGG ATAACAGTGT CATCCTGGCA
    ATGCTGGAGC AACCTCTTGG AAATGAACAG AATGATTTTT TTCCCTCTGT CACTGTGCTG
    GTTCGAGGAA TGTCTGGAAG ACTTGCTTGG GCACAACAAC TTTGCCTTTT ACCCAGAGGA
    GCAAAAGCAA ATCAGAAGCT TTTTGTACCT GAACCTCGTC CAATTCCTAA AAATGATGTT
    GGATTTAAAT ATACTGTCAA ACATCGACCA TTTCCTGAAG AGGTGGATAA GATTCCTTTT
    GTGAAAGCAG ATCTGAGCAT TCCAGATTTG CATGAAATTG TCACTGAAGA ATTAGAAGAG
    AGACATGAAA AATTAAGGAA TGGCATGGCC CAGCAGATTG CTTATGAAAT ACACCTTGAA
    CAACAAAGTG AGGAAGAATT GCAGAAGAGA AGTTTTCCTG ATCCAATTAC GGATTGTAAG
    CCTCCACCTC CTGCCCAAGA ATTCCAAACA GCACGCCTCT TCCTTTCACA CTTTGGATTT
    TTGTCTTTAG AAGCATTGAA GGAACCTGCA AATAGTCGCC TACCTCCTCA CCTTATTGCA
    CTTGACTCCA CGATACCTGG ATTTTTTGAT GACATTGGAT ATCTGGATCT CTTGCCGTGT
    CGTCCCTTTG ACACAGTTTT TATTTTCTAT ATGAAACCAG GTCAGAAAAC AAACCAGGAG
    ATTTTAAAGA ATGTGGAATC TTCCAGAAAT GTTCAGCCAC ATTTCCTAGA ATTTTTGCTC
    TCCCTTGGCT GGTCAGTAGA TGTGGGCAGA CACCCAGGTT GGACTGGGCA TGTTTCTACC
    AGTTGGTCTA TTAACAGTTG TGATGATGGT GAAGGACCTG AACAAGATGA AGTGATTTCC
    TCTGAAGACA TTGGGGCTAG CATTTTCAAT GGACAGAAGA AGGTGCTGTA TTATGCTGAT
    GCCCTTACGG AAATAGCTTT TGTAGTTCCT TCTCCTGTGG AGTCTTTAAC TGATTCATTA
    GAAAGTAACA TCTCGGACCA AGATAGTGAT TCAAATATGG ATCTTATGCC TGGAATTCTG
    AAACAGCCAT CCCTGACACT TGAGCTTTTC CCTAATCACA CAGACAATCT TAATTCCTCA
    CAGCGGCTTA GTCCCAGTTC CAGAATGAAG AAGCTGCCTC AGGGTCGCCC TGTGCCTCCC
    CTTGGACCTG AGACAAGAGT TTCTGTAGTC TGGGTGGAAC GCTATGATGA TATAGAGAAC
    TTTCCCCTCT CAGACCTGCT GACAGAGATC AGTACTGGTG TGGAAACTAC TGCAAACAGC
    AGCACTTCTC TGAGATCTAC CACTCTTGAA AAAGAAGTTC CTGTCATCTT TATCCACCCT
    TTAAACACTG GATTATTCCG GATAAAAATT CAAGGAGCCA CTGGAAAATT TAACATGGTC
    ATCCCGCTCG TGGATGGGAT GATTGTCAGC AGACGAGCAC TTGGTTTTCT GGTGAGGCAG
    ACTGTAATTA ATATTTGTAG AAGAAAGAGA CTGGAGAGTG ACTCCTACAG TCCACCACAT
    GTCCGCCGGA AACAGAAAAT CACCGACATT GTCAACAAGT ACCGGAACAA GCAGTTGGAG
    CCAGAGTTTT ATACTTCACT TTTCCAGGAG GTTGGACTCA AGAACTGCTC TTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe24892
    Clone ID Related Accession (Same CDS sequence) XM_005635054.2 , XM_022409445.1
    Accession Version XM_022409445.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4497bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ral GTPase-activating protein subunit beta isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATCCAGA ATGATCAGGG CCACACGAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGG CGAGAGGTGG CAAATGCAGT GGTCCATCCC
    CTTGGGCAGG CCTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAGTTTGTT AAAAACTGAC
    AAAGAAGTGA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGTGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTATTGCCG
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC TATATGTTCA AAGTATACTA
    AAACACCTGC AAAATCTTTT TGTACCAAGA CAGGAACAGG GCTCCAGTCA GATCCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTAGCCCGTG AGTCATCCAT CATGGCACGA
    GAAACGTGGG AAGTCTTACT ATTGTTTCTT CTGCAGATTA ATGACATACT TCTAGCCCCG
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTTTGGT TACTAGCTTG TACTCGGTGC TTTCCAACAC CTCCTTATTG GAAAACCGCC
    AAGGAGATGG TGGCAAACTG GAGGCACCAC CCGGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ACCTGTGCCC TCACTTCAAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTCCATTT
    AAAGTTCCTG ATGAAGATGC CAGTCTGATC CCTCCTGAAA TGGATAATGA GTGTGTAGCA
    CAGACATGGT TTCGCTTCTT ACATATGTTA AGTAATCCCG TGGATTTGAG TAACCCGGCC
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTAA ATGTGAGCGG AATGCCACAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTAGTGGA TGCATTCTTA GGCATTTCTA GACCCCGATC AGACAGTGCT
    CCTCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGCA CTGCTGTCAG TACCACCCCA
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC GAAAGTTCAG CACCAGACAT CCTCCACCTC TCCTCTGTCA
    AGTCCCAATC AGACTAGTTC TGAACCCCGG CCACTGCCTG CCCCTCGAAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCCA TTACAACACA AGCTAGCATG
    GAGTTTCGAC GGAAAGGGTC ACAAATGTCC ACAGACACCA TGGTTTCCAA TCCTATGTTT
    GATGCAAGTG AATTTCCTGA TAACTATGAA GCAGGAAGAG CTGAGGCTTG TGGGACACTG
    TGTAGGATTT TTTGTAGCAA GAAGACTGGA GAAGAGATTC TGCCAGCTTA TTTATCCAGA
    TTTTACATGC TTTTAATTCA AGGTTTGCAG ATAAATGATT ATGTGTGCCA TCCTGTCTTG
    GCCAGCGTTA TTCTAAACTC CCCTCCTTTG TTCTGCTGTG ACTTGAAAGG GATTGATGTT
    GTGGTTCCTT ACTTTATTTC AGCTCTTGAA ACCATTTTGC CTGACAGAGA ACTCTCAAAA
    TTCAAAAGCT ATGTAAATCC AACAGAATTG AGAAGATCTT CCATTAATAT CCTCCTTTCT
    TTATTGCCCC TCCCTCATCA TTTTGGCACA GTCAGATCTG AGGTGGTCCT GGAAGGGAAG
    TTTAGTAATG ATGACAGCTC TTCATATGAT AAACCAATAA CTTTTTTGTC CCTGAAGTTG
    AGACTTGTGA ATATACTAAT AGGTGCCTTG CAAACGGAAA CGGACCCCAA CAACACTCAA
    ATGATACTAG GGGCAATGTT AAATATTGTT CAAGATTCAG CACTTTTAGA AGCCATTGGT
    TGCCAAATGG AGATGGGTGG TGGAGAAAAT AACCTGAAGA GTCATAGTCG TACTAATAGT
    GGTATTAGTT CAGCAAGTGG TGGAAGCACA GAACCCACAA CTCCTGATAG TGAAAGACCT
    GCTCAAGCTC TCCTAAGGGA TTATGGATCG ACAGCTCTTA ATACAGATTC AGCTGCTGGG
    CTCCTGATTC GCAGCATTCA TCTTGTCACC CAAAGACTCA ACTCTCAGTG GCGGCAAGAC
    ATGAGCATAT CACTGGCAGC GCTGGAGCTC CTCTCTGGCC TGGCAAAGGT GAAGGTGATG
    GTTGACTCGG GAGACCGGAA ACGAGCCATC AGTTCTGTGT GCAGCTACAT CGTGTACCAA
    TGTAGTCGGC CAGCTCCTCT TCACTCCCGC GATCTGCACT CCATGATAGT AGCAGCTTTT
    CAGTGTCTCT GTGTCTGGCT GACAGAGCAT CCTGATATGC TTGATGAAAA GGACTGTCTT
    AAGGAAGTAT TGGAGATTGT GGAACTGGGT ATTTCAGGAA GTAAGTCCAA GAACAGTGAG
    CAAGAGGTCA AGTACAAAGG AGATAAGGAG CCAAACCCTG CATCTATGAG GGTAAAGGAT
    GCTGCTGAAG CCACTCTAAC ATGTATTATG CAGTTGCTCG GCGCGTTTCC TTCACCCAGT
    GGTCCTGCCT CTCCTTGTAG TCTGGTGAAT GAGACCACTT TGATTAAATA CTCAAGGCTG
    CCAACCATAA ACAAGCATAG TTTCCGGTAC TTTGTCTTGG ATAACAGTGT CATCCTGGCA
    ATGCTGGAGC AACCTCTTGG AAATGAACAG AATGATTTTT TTCCCTCTGT CACTGTGCTG
    GTTCGAGGAA TGTCTGGAAG ACTTGCTTGG GCACAACAAC TTTGCCTTTT ACCCAGAGGA
    GCAAAAGCAA ATCAGAAGCT TTTTGTACCT GAACCTCGTC CAATTCCTAA AAATGATGTT
    GGATTTAAAT ATACTGTCAA ACATCGACCA TTTCCTGAAG AGGTGGATAA GATTCCTTTT
    GTGAAAGCAG ATCTGAGCAT TCCAGATTTG CATGAAATTG TCACTGAAGA ATTAGAAGAG
    AGACATGAAA AATTAAGGAA TGGCATGGCC CAGCAGATTG CTTATGAAAT ACACCTTGAA
    CAACAAAGTG AGGAAGAATT GCAGAAGAGA AGTTTTCCTG ATCCAATTAC GGATTGTAAG
    CCTCCACCTC CTGCCCAAGA ATTCCAAACA GCACGCCTCT TCCTTTCACA CTTTGGATTT
    TTGTCTTTAG AAGCATTGAA GGAACCTGCA AATAGTCGCC TACCTCCTCA CCTTATTGCA
    CTTGACTCCA CGATACCTGG ATTTTTTGAT GACATTGGAT ATCTGGATCT CTTGCCGTGT
    CGTCCCTTTG ACACAGTTTT TATTTTCTAT ATGAAACCAG GTCAGAAAAC AAACCAGGAG
    ATTTTAAAGA ATGTGGAATC TTCCAGAAAT GTTCAGCCAC ATTTCCTAGA ATTTTTGCTC
    TCCCTTGGCT GGTCAGTAGA TGTGGGCAGA CACCCAGGTT GGACTGGGCA TGTTTCTACC
    AGTTGGTCTA TTAACAGTTG TGATGATGGT GAAGGACCTG AACAAGATGA AGTGATTTCC
    TCTGAAGACA TTGGGGCTAG CATTTTCAAT GGACAGAAGA AGGTGCTGTA TTATGCTGAT
    GCCCTTACGG AAATAGCTTT TGTAGTTCCT TCTCCTGTGG AGTCTTTAAC TGATTCATTA
    GAAAGTAACA TCTCGGACCA AGATAGTGAT TCAAATATGG ATCTTATGCC TGGAATTCTG
    AAACAGCCAT CCCTGACACT TGAGCTTTTC CCTAATCACA CAGACAATCT TAATTCCTCA
    CAGCGGCTTA GTCCCAGTTC CAGAATGAAG AAGCTGCCTC AGGGTCGCCC TGTGCCTCCC
    CTTGGACCTG AGACAAGAGT TTCTGTAGTC TGGGTGGAAC GCTATGATGA TATAGAGAAC
    TTTCCCCTCT CAGACCTGCT GACAGAGATC AGTACTGGTG TGGAAACTAC TGCAAACAGC
    AGCACTTCTC TGAGATCTAC CACTCTTGAA AAAGAAGTTC CTGTCATCTT TATCCACCCT
    TTAAACACTG GATTATTCCG GATAAAAATT CAAGGAGCCA CTGGAAAATT TAACATGGTC
    ATCCCGCTCG TGGATGGGAT GATTGTCAGC AGACGAGCAC TTGGTTTTCT GGTGAGGCAG
    ACTGTAATTA ATATTTGTAG AAGAAAGAGA CTGGAGAGTG ACTCCTACAG TCCACCACAT
    GTCCGCCGGA AACAGAAAAT CACCGACATT GTCAACAAGT ACCGGAACAA GCAGTTGGAG
    CCAGAGTTTT ATACTTCACT TTTCCAGGAG GTTGGACTCA AGAACTGCTC TTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022265153.1
    CDS368..4864
    Translation

    Target ORF information:

    RefSeq Version XM_022409445.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022409445.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATCCAGA ATGATCAGGG CCACACGAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGG CGAGAGGTGG CAAATGCAGT GGTCCATCCC
    CTTGGGCAGG CCTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAGTTTGTT AAAAACTGAC
    AAAGAAGTGA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGTGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTATTGCCG
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC TATATGTTCA AAGTATACTA
    AAACACCTGC AAAATCTTTT TGTACCAAGA CAGGAACAGG GCTCCAGTCA GATCCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTAGCCCGTG AGTCATCCAT CATGGCACGA
    GAAACGTGGG AAGTCTTACT ATTGTTTCTT CTGCAGATTA ATGACATACT TCTAGCCCCG
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTTTGGT TACTAGCTTG TACTCGGTGC TTTCCAACAC CTCCTTATTG GAAAACCGCC
    AAGGAGATGG TGGCAAACTG GAGGCACCAC CCGGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ACCTGTGCCC TCACTTCAAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTCCATTT
    AAAGTTCCTG ATGAAGATGC CAGTCTGATC CCTCCTGAAA TGGATAATGA GTGTGTAGCA
    CAGACATGGT TTCGCTTCTT ACATATGTTA AGTAATCCCG TGGATTTGAG TAACCCGGCC
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTAA ATGTGAGCGG AATGCCACAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTAGTGGA TGCATTCTTA GGCATTTCTA GACCCCGATC AGACAGTGCT
    CCTCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGCA CTGCTGTCAG TACCACCCCA
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC GAAAGTTCAG CACCAGACAT CCTCCACCTC TCCTCTGTCA
    AGTCCCAATC AGACTAGTTC TGAACCCCGG CCACTGCCTG CCCCTCGAAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCCA TTACAACACA AGCTAGCATG
    GAGTTTCGAC GGAAAGGGTC ACAAATGTCC ACAGACACCA TGGTTTCCAA TCCTATGTTT
    GATGCAAGTG AATTTCCTGA TAACTATGAA GCAGGAAGAG CTGAGGCTTG TGGGACACTG
    TGTAGGATTT TTTGTAGCAA GAAGACTGGA GAAGAGATTC TGCCAGCTTA TTTATCCAGA
    TTTTACATGC TTTTAATTCA AGGTTTGCAG ATAAATGATT ATGTGTGCCA TCCTGTCTTG
    GCCAGCGTTA TTCTAAACTC CCCTCCTTTG TTCTGCTGTG ACTTGAAAGG GATTGATGTT
    GTGGTTCCTT ACTTTATTTC AGCTCTTGAA ACCATTTTGC CTGACAGAGA ACTCTCAAAA
    TTCAAAAGCT ATGTAAATCC AACAGAATTG AGAAGATCTT CCATTAATAT CCTCCTTTCT
    TTATTGCCCC TCCCTCATCA TTTTGGCACA GTCAGATCTG AGGTGGTCCT GGAAGGGAAG
    TTTAGTAATG ATGACAGCTC TTCATATGAT AAACCAATAA CTTTTTTGTC CCTGAAGTTG
    AGACTTGTGA ATATACTAAT AGGTGCCTTG CAAACGGAAA CGGACCCCAA CAACACTCAA
    ATGATACTAG GGGCAATGTT AAATATTGTT CAAGATTCAG CACTTTTAGA AGCCATTGGT
    TGCCAAATGG AGATGGGTGG TGGAGAAAAT AACCTGAAGA GTCATAGTCG TACTAATAGT
    GGTATTAGTT CAGCAAGTGG TGGAAGCACA GAACCCACAA CTCCTGATAG TGAAAGACCT
    GCTCAAGCTC TCCTAAGGGA TTATGGATCG ACAGCTCTTA ATACAGATTC AGCTGCTGGG
    CTCCTGATTC GCAGCATTCA TCTTGTCACC CAAAGACTCA ACTCTCAGTG GCGGCAAGAC
    ATGAGCATAT CACTGGCAGC GCTGGAGCTC CTCTCTGGCC TGGCAAAGGT GAAGGTGATG
    GTTGACTCGG GAGACCGGAA ACGAGCCATC AGTTCTGTGT GCAGCTACAT CGTGTACCAA
    TGTAGTCGGC CAGCTCCTCT TCACTCCCGC GATCTGCACT CCATGATAGT AGCAGCTTTT
    CAGTGTCTCT GTGTCTGGCT GACAGAGCAT CCTGATATGC TTGATGAAAA GGACTGTCTT
    AAGGAAGTAT TGGAGATTGT GGAACTGGGT ATTTCAGGAA GTAAGTCCAA GAACAGTGAG
    CAAGAGGTCA AGTACAAAGG AGATAAGGAG CCAAACCCTG CATCTATGAG GGTAAAGGAT
    GCTGCTGAAG CCACTCTAAC ATGTATTATG CAGTTGCTCG GCGCGTTTCC TTCACCCAGT
    GGTCCTGCCT CTCCTTGTAG TCTGGTGAAT GAGACCACTT TGATTAAATA CTCAAGGCTG
    CCAACCATAA ACAAGCATAG TTTCCGGTAC TTTGTCTTGG ATAACAGTGT CATCCTGGCA
    ATGCTGGAGC AACCTCTTGG AAATGAACAG AATGATTTTT TTCCCTCTGT CACTGTGCTG
    GTTCGAGGAA TGTCTGGAAG ACTTGCTTGG GCACAACAAC TTTGCCTTTT ACCCAGAGGA
    GCAAAAGCAA ATCAGAAGCT TTTTGTACCT GAACCTCGTC CAATTCCTAA AAATGATGTT
    GGATTTAAAT ATACTGTCAA ACATCGACCA TTTCCTGAAG AGGTGGATAA GATTCCTTTT
    GTGAAAGCAG ATCTGAGCAT TCCAGATTTG CATGAAATTG TCACTGAAGA ATTAGAAGAG
    AGACATGAAA AATTAAGGAA TGGCATGGCC CAGCAGATTG CTTATGAAAT ACACCTTGAA
    CAACAAAGTG AGGAAGAATT GCAGAAGAGA AGTTTTCCTG ATCCAATTAC GGATTGTAAG
    CCTCCACCTC CTGCCCAAGA ATTCCAAACA GCACGCCTCT TCCTTTCACA CTTTGGATTT
    TTGTCTTTAG AAGCATTGAA GGAACCTGCA AATAGTCGCC TACCTCCTCA CCTTATTGCA
    CTTGACTCCA CGATACCTGG ATTTTTTGAT GACATTGGAT ATCTGGATCT CTTGCCGTGT
    CGTCCCTTTG ACACAGTTTT TATTTTCTAT ATGAAACCAG GTCAGAAAAC AAACCAGGAG
    ATTTTAAAGA ATGTGGAATC TTCCAGAAAT GTTCAGCCAC ATTTCCTAGA ATTTTTGCTC
    TCCCTTGGCT GGTCAGTAGA TGTGGGCAGA CACCCAGGTT GGACTGGGCA TGTTTCTACC
    AGTTGGTCTA TTAACAGTTG TGATGATGGT GAAGGACCTG AACAAGATGA AGTGATTTCC
    TCTGAAGACA TTGGGGCTAG CATTTTCAAT GGACAGAAGA AGGTGCTGTA TTATGCTGAT
    GCCCTTACGG AAATAGCTTT TGTAGTTCCT TCTCCTGTGG AGTCTTTAAC TGATTCATTA
    GAAAGTAACA TCTCGGACCA AGATAGTGAT TCAAATATGG ATCTTATGCC TGGAATTCTG
    AAACAGCCAT CCCTGACACT TGAGCTTTTC CCTAATCACA CAGACAATCT TAATTCCTCA
    CAGCGGCTTA GTCCCAGTTC CAGAATGAAG AAGCTGCCTC AGGGTCGCCC TGTGCCTCCC
    CTTGGACCTG AGACAAGAGT TTCTGTAGTC TGGGTGGAAC GCTATGATGA TATAGAGAAC
    TTTCCCCTCT CAGACCTGCT GACAGAGATC AGTACTGGTG TGGAAACTAC TGCAAACAGC
    AGCACTTCTC TGAGATCTAC CACTCTTGAA AAAGAAGTTC CTGTCATCTT TATCCACCCT
    TTAAACACTG GATTATTCCG GATAAAAATT CAAGGAGCCA CTGGAAAATT TAACATGGTC
    ATCCCGCTCG TGGATGGGAT GATTGTCAGC AGACGAGCAC TTGGTTTTCT GGTGAGGCAG
    ACTGTAATTA ATATTTGTAG AAGAAAGAGA CTGGAGAGTG ACTCCTACAG TCCACCACAT
    GTCCGCCGGA AACAGAAAAT CACCGACATT GTCAACAAGT ACCGGAACAA GCAGTTGGAG
    CCAGAGTTTT ATACTTCACT TTTCCAGGAG GTTGGACTCA AGAACTGCTC TTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe24893
    Clone ID Related Accession (Same CDS sequence) XM_022409446.1 , XM_847511.4
    Accession Version XM_022409446.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4488bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ral GTPase-activating protein subunit beta isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATCCAGA ATGATCAGGG CCACACGAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGG CGAGAGGTGG CAAATGCAGT GGTCCATCCC
    CTTGGGCAGG CCTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAGTTTGTT AAAAACTGAC
    AAAGAAGTGA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGTGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTATTGCCG
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC TATATGTTCA AAGTATACTA
    AAACACCTGC AAAATCTTTT TGTACCAAGA CAGGAACAGG GCTCCAGTCA GATCCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTAGCCCGTG AGTCATCCAT CATGGCACGA
    GAAACGTGGG AAGTCTTACT ATTGTTTCTT CTGCAGATTA ATGACATACT TCTAGCCCCG
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTTTGGT TACTAGCTTG TACTCGGTGC TTTCCAACAC CTCCTTATTG GAAAACCGCC
    AAGGAGATGG TGGCAAACTG GAGGCACCAC CCGGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ACCTGTGCCC TCACTTCAAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTCCATTT
    AAAGTTCCTG ATGAAGATGC CAGTCTGATC CCTCCTGAAA TGGATAATGA GTGTGTAGCA
    CAGACATGGT TTCGCTTCTT ACATATGTTA AGTAATCCCG TGGATTTGAG TAACCCGGCC
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTAA ATGTGAGCGG AATGCCACAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTAGTGGA TGCATTCTTA GGCATTTCTA GACCCCGATC AGACAGTGCT
    CCTCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGCA CTGCTGTCAG TACCACCCCA
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC GAAAGTTCAG CACCAGACAT CCTCCACCTC TCCTCTGTCA
    AGTCCCAATC AGACTAGTTC TGAACCCCGG CCACTGCCTG CCCCTCGAAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCCA TTACAACACA AGCTAGCATG
    GAGTTTCGAC GGAAAGGGTC ACAAATGTCC ACAGACACCA TGGTTTCCAA TCCTATGTTT
    GATGCAAGTG AATTTCCTGA TAACTATGAA GCAGGAAGAG CTGAGGCTTG TGGGACACTG
    TGTAGGATTT TTTGTAGCAA GAAGACTGGA GAAGAGATTC TGCCAGCTTA TTTATCCAGA
    TTTTACATGC TTTTAATTCA AGGTTTGCAG ATAAATGATT ATGTGTGCCA TCCTGTCTTG
    GCCAGCGTTA TTCTAAACTC CCCTCCTTTG TTCTGCTGTG ACTTGAAAGG GATTGATGTT
    GTGGTTCCTT ACTTTATTTC AGCTCTTGAA ACCATTTTGC CTGACAGAGA ACTCTCAAAA
    TTCAAAAGCT ATGTAAATCC AACAGAATTG AGAAGATCTT CCATTAATAT CCTCCTTTCT
    TTATTGCCCC TCCCTCATCA TTTTGGCACA GTCAGATCTG AGGTGGTCCT GGAAGGGAAG
    TTTAGTAATG ATGACAGCTC TTCATATGAT AAACCAATAA CTTTTTTGTC CCTGAAGTTG
    AGACTTGTGA ATATACTAAT AGGTGCCTTG CAAACGGAAA CGGACCCCAA CAACACTCAA
    ATGATACTAG GGGCAATGTT AAATATTGTT CAAGATTCAG CACTTTTAGA AGCCATTGGT
    TGCCAAATGG AGATGGGTGG TGGAGAAAAT AACCTGAAGA GTCATAGTCG TACTAATAGT
    GGTATTAGTT CAGCAAGTGG TGGAAGCACA GAACCCACAA CTCCTGATAG TGAAAGACCT
    GCTCAAGCTC TCCTAAGGGA TTATGCTCTT AATACAGATT CAGCTGCTGG GCTCCTGATT
    CGCAGCATTC ATCTTGTCAC CCAAAGACTC AACTCTCAGT GGCGGCAAGA CATGAGCATA
    TCACTGGCAG CGCTGGAGCT CCTCTCTGGC CTGGCAAAGG TGAAGGTGAT GGTTGACTCG
    GGAGACCGGA AACGAGCCAT CAGTTCTGTG TGCAGCTACA TCGTGTACCA ATGTAGTCGG
    CCAGCTCCTC TTCACTCCCG CGATCTGCAC TCCATGATAG TAGCAGCTTT TCAGTGTCTC
    TGTGTCTGGC TGACAGAGCA TCCTGATATG CTTGATGAAA AGGACTGTCT TAAGGAAGTA
    TTGGAGATTG TGGAACTGGG TATTTCAGGA AGTAAGTCCA AGAACAGTGA GCAAGAGGTC
    AAGTACAAAG GAGATAAGGA GCCAAACCCT GCATCTATGA GGGTAAAGGA TGCTGCTGAA
    GCCACTCTAA CATGTATTAT GCAGTTGCTC GGCGCGTTTC CTTCACCCAG TGGTCCTGCC
    TCTCCTTGTA GTCTGGTGAA TGAGACCACT TTGATTAAAT ACTCAAGGCT GCCAACCATA
    AACAAGCATA GTTTCCGGTA CTTTGTCTTG GATAACAGTG TCATCCTGGC AATGCTGGAG
    CAACCTCTTG GAAATGAACA GAATGATTTT TTTCCCTCTG TCACTGTGCT GGTTCGAGGA
    ATGTCTGGAA GACTTGCTTG GGCACAACAA CTTTGCCTTT TACCCAGAGG AGCAAAAGCA
    AATCAGAAGC TTTTTGTACC TGAACCTCGT CCAATTCCTA AAAATGATGT TGGATTTAAA
    TATACTGTCA AACATCGACC ATTTCCTGAA GAGGTGGATA AGATTCCTTT TGTGAAAGCA
    GATCTGAGCA TTCCAGATTT GCATGAAATT GTCACTGAAG AATTAGAAGA GAGACATGAA
    AAATTAAGGA ATGGCATGGC CCAGCAGATT GCTTATGAAA TACACCTTGA ACAACAAAGT
    GAGGAAGAAT TGCAGAAGAG AAGTTTTCCT GATCCAATTA CGGATTGTAA GCCTCCACCT
    CCTGCCCAAG AATTCCAAAC AGCACGCCTC TTCCTTTCAC ACTTTGGATT TTTGTCTTTA
    GAAGCATTGA AGGAACCTGC AAATAGTCGC CTACCTCCTC ACCTTATTGC ACTTGACTCC
    ACGATACCTG GATTTTTTGA TGACATTGGA TATCTGGATC TCTTGCCGTG TCGTCCCTTT
    GACACAGTTT TTATTTTCTA TATGAAACCA GGTCAGAAAA CAAACCAGGA GATTTTAAAG
    AATGTGGAAT CTTCCAGAAA TGTTCAGCCA CATTTCCTAG AATTTTTGCT CTCCCTTGGC
    TGGTCAGTAG ATGTGGGCAG ACACCCAGGT TGGACTGGGC ATGTTTCTAC CAGTTGGTCT
    ATTAACAGTT GTGATGATGG TGAAGGACCT GAACAAGATG AAGTGATTTC CTCTGAAGAC
    ATTGGGGCTA GCATTTTCAA TGGACAGAAG AAGGTGCTGT ATTATGCTGA TGCCCTTACG
    GAAATAGCTT TTGTAGTTCC TTCTCCTGTG GAGTCTTTAA CTGATTCATT AGAAAGTAAC
    ATCTCGGACC AAGATAGTGA TTCAAATATG GATCTTATGC CTGGAATTCT GAAACAGCCA
    TCCCTGACAC TTGAGCTTTT CCCTAATCAC ACAGACAATC TTAATTCCTC ACAGCGGCTT
    AGTCCCAGTT CCAGAATGAA GAAGCTGCCT CAGGGTCGCC CTGTGCCTCC CCTTGGACCT
    GAGACAAGAG TTTCTGTAGT CTGGGTGGAA CGCTATGATG ATATAGAGAA CTTTCCCCTC
    TCAGACCTGC TGACAGAGAT CAGTACTGGT GTGGAAACTA CTGCAAACAG CAGCACTTCT
    CTGAGATCTA CCACTCTTGA AAAAGAAGTT CCTGTCATCT TTATCCACCC TTTAAACACT
    GGATTATTCC GGATAAAAAT TCAAGGAGCC ACTGGAAAAT TTAACATGGT CATCCCGCTC
    GTGGATGGGA TGATTGTCAG CAGACGAGCA CTTGGTTTTC TGGTGAGGCA GACTGTAATT
    AATATTTGTA GAAGAAAGAG ACTGGAGAGT GACTCCTACA GTCCACCACA TGTCCGCCGG
    AAACAGAAAA TCACCGACAT TGTCAACAAG TACCGGAACA AGCAGTTGGA GCCAGAGTTT
    TATACTTCAC TTTTCCAGGA GGTTGGACTC AAGAACTGCT CTTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022265154.1
    CDS367..4854
    Translation

    Target ORF information:

    RefSeq Version XM_022409446.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022409446.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATCCAGA ATGATCAGGG CCACACGAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGG CGAGAGGTGG CAAATGCAGT GGTCCATCCC
    CTTGGGCAGG CCTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAGTTTGTT AAAAACTGAC
    AAAGAAGTGA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGTGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTATTGCCG
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC TATATGTTCA AAGTATACTA
    AAACACCTGC AAAATCTTTT TGTACCAAGA CAGGAACAGG GCTCCAGTCA GATCCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTAGCCCGTG AGTCATCCAT CATGGCACGA
    GAAACGTGGG AAGTCTTACT ATTGTTTCTT CTGCAGATTA ATGACATACT TCTAGCCCCG
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTTTGGT TACTAGCTTG TACTCGGTGC TTTCCAACAC CTCCTTATTG GAAAACCGCC
    AAGGAGATGG TGGCAAACTG GAGGCACCAC CCGGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ACCTGTGCCC TCACTTCAAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTCCATTT
    AAAGTTCCTG ATGAAGATGC CAGTCTGATC CCTCCTGAAA TGGATAATGA GTGTGTAGCA
    CAGACATGGT TTCGCTTCTT ACATATGTTA AGTAATCCCG TGGATTTGAG TAACCCGGCC
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTAA ATGTGAGCGG AATGCCACAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTAGTGGA TGCATTCTTA GGCATTTCTA GACCCCGATC AGACAGTGCT
    CCTCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGCA CTGCTGTCAG TACCACCCCA
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC GAAAGTTCAG CACCAGACAT CCTCCACCTC TCCTCTGTCA
    AGTCCCAATC AGACTAGTTC TGAACCCCGG CCACTGCCTG CCCCTCGAAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCCA TTACAACACA AGCTAGCATG
    GAGTTTCGAC GGAAAGGGTC ACAAATGTCC ACAGACACCA TGGTTTCCAA TCCTATGTTT
    GATGCAAGTG AATTTCCTGA TAACTATGAA GCAGGAAGAG CTGAGGCTTG TGGGACACTG
    TGTAGGATTT TTTGTAGCAA GAAGACTGGA GAAGAGATTC TGCCAGCTTA TTTATCCAGA
    TTTTACATGC TTTTAATTCA AGGTTTGCAG ATAAATGATT ATGTGTGCCA TCCTGTCTTG
    GCCAGCGTTA TTCTAAACTC CCCTCCTTTG TTCTGCTGTG ACTTGAAAGG GATTGATGTT
    GTGGTTCCTT ACTTTATTTC AGCTCTTGAA ACCATTTTGC CTGACAGAGA ACTCTCAAAA
    TTCAAAAGCT ATGTAAATCC AACAGAATTG AGAAGATCTT CCATTAATAT CCTCCTTTCT
    TTATTGCCCC TCCCTCATCA TTTTGGCACA GTCAGATCTG AGGTGGTCCT GGAAGGGAAG
    TTTAGTAATG ATGACAGCTC TTCATATGAT AAACCAATAA CTTTTTTGTC CCTGAAGTTG
    AGACTTGTGA ATATACTAAT AGGTGCCTTG CAAACGGAAA CGGACCCCAA CAACACTCAA
    ATGATACTAG GGGCAATGTT AAATATTGTT CAAGATTCAG CACTTTTAGA AGCCATTGGT
    TGCCAAATGG AGATGGGTGG TGGAGAAAAT AACCTGAAGA GTCATAGTCG TACTAATAGT
    GGTATTAGTT CAGCAAGTGG TGGAAGCACA GAACCCACAA CTCCTGATAG TGAAAGACCT
    GCTCAAGCTC TCCTAAGGGA TTATGCTCTT AATACAGATT CAGCTGCTGG GCTCCTGATT
    CGCAGCATTC ATCTTGTCAC CCAAAGACTC AACTCTCAGT GGCGGCAAGA CATGAGCATA
    TCACTGGCAG CGCTGGAGCT CCTCTCTGGC CTGGCAAAGG TGAAGGTGAT GGTTGACTCG
    GGAGACCGGA AACGAGCCAT CAGTTCTGTG TGCAGCTACA TCGTGTACCA ATGTAGTCGG
    CCAGCTCCTC TTCACTCCCG CGATCTGCAC TCCATGATAG TAGCAGCTTT TCAGTGTCTC
    TGTGTCTGGC TGACAGAGCA TCCTGATATG CTTGATGAAA AGGACTGTCT TAAGGAAGTA
    TTGGAGATTG TGGAACTGGG TATTTCAGGA AGTAAGTCCA AGAACAGTGA GCAAGAGGTC
    AAGTACAAAG GAGATAAGGA GCCAAACCCT GCATCTATGA GGGTAAAGGA TGCTGCTGAA
    GCCACTCTAA CATGTATTAT GCAGTTGCTC GGCGCGTTTC CTTCACCCAG TGGTCCTGCC
    TCTCCTTGTA GTCTGGTGAA TGAGACCACT TTGATTAAAT ACTCAAGGCT GCCAACCATA
    AACAAGCATA GTTTCCGGTA CTTTGTCTTG GATAACAGTG TCATCCTGGC AATGCTGGAG
    CAACCTCTTG GAAATGAACA GAATGATTTT TTTCCCTCTG TCACTGTGCT GGTTCGAGGA
    ATGTCTGGAA GACTTGCTTG GGCACAACAA CTTTGCCTTT TACCCAGAGG AGCAAAAGCA
    AATCAGAAGC TTTTTGTACC TGAACCTCGT CCAATTCCTA AAAATGATGT TGGATTTAAA
    TATACTGTCA AACATCGACC ATTTCCTGAA GAGGTGGATA AGATTCCTTT TGTGAAAGCA
    GATCTGAGCA TTCCAGATTT GCATGAAATT GTCACTGAAG AATTAGAAGA GAGACATGAA
    AAATTAAGGA ATGGCATGGC CCAGCAGATT GCTTATGAAA TACACCTTGA ACAACAAAGT
    GAGGAAGAAT TGCAGAAGAG AAGTTTTCCT GATCCAATTA CGGATTGTAA GCCTCCACCT
    CCTGCCCAAG AATTCCAAAC AGCACGCCTC TTCCTTTCAC ACTTTGGATT TTTGTCTTTA
    GAAGCATTGA AGGAACCTGC AAATAGTCGC CTACCTCCTC ACCTTATTGC ACTTGACTCC
    ACGATACCTG GATTTTTTGA TGACATTGGA TATCTGGATC TCTTGCCGTG TCGTCCCTTT
    GACACAGTTT TTATTTTCTA TATGAAACCA GGTCAGAAAA CAAACCAGGA GATTTTAAAG
    AATGTGGAAT CTTCCAGAAA TGTTCAGCCA CATTTCCTAG AATTTTTGCT CTCCCTTGGC
    TGGTCAGTAG ATGTGGGCAG ACACCCAGGT TGGACTGGGC ATGTTTCTAC CAGTTGGTCT
    ATTAACAGTT GTGATGATGG TGAAGGACCT GAACAAGATG AAGTGATTTC CTCTGAAGAC
    ATTGGGGCTA GCATTTTCAA TGGACAGAAG AAGGTGCTGT ATTATGCTGA TGCCCTTACG
    GAAATAGCTT TTGTAGTTCC TTCTCCTGTG GAGTCTTTAA CTGATTCATT AGAAAGTAAC
    ATCTCGGACC AAGATAGTGA TTCAAATATG GATCTTATGC CTGGAATTCT GAAACAGCCA
    TCCCTGACAC TTGAGCTTTT CCCTAATCAC ACAGACAATC TTAATTCCTC ACAGCGGCTT
    AGTCCCAGTT CCAGAATGAA GAAGCTGCCT CAGGGTCGCC CTGTGCCTCC CCTTGGACCT
    GAGACAAGAG TTTCTGTAGT CTGGGTGGAA CGCTATGATG ATATAGAGAA CTTTCCCCTC
    TCAGACCTGC TGACAGAGAT CAGTACTGGT GTGGAAACTA CTGCAAACAG CAGCACTTCT
    CTGAGATCTA CCACTCTTGA AAAAGAAGTT CCTGTCATCT TTATCCACCC TTTAAACACT
    GGATTATTCC GGATAAAAAT TCAAGGAGCC ACTGGAAAAT TTAACATGGT CATCCCGCTC
    GTGGATGGGA TGATTGTCAG CAGACGAGCA CTTGGTTTTC TGGTGAGGCA GACTGTAATT
    AATATTTGTA GAAGAAAGAG ACTGGAGAGT GACTCCTACA GTCCACCACA TGTCCGCCGG
    AAACAGAAAA TCACCGACAT TGTCAACAAG TACCGGAACA AGCAGTTGGA GCCAGAGTTT
    TATACTTCAC TTTTCCAGGA GGTTGGACTC AAGAACTGCT CTTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe24893
    Clone ID Related Accession (Same CDS sequence) XM_022409446.1 , XM_847511.4
    Accession Version XM_847511.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4488bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ral GTPase-activating protein subunit beta isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726101.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_847511.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATCCAGA ATGATCAGGG CCACACGAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGG CGAGAGGTGG CAAATGCAGT GGTCCATCCC
    CTTGGGCAGG CCTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAGTTTGTT AAAAACTGAC
    AAAGAAGTGA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGTGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTATTGCCG
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC TATATGTTCA AAGTATACTA
    AAACACCTGC AAAATCTTTT TGTACCAAGA CAGGAACAGG GCTCCAGTCA GATCCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTAGCCCGTG AGTCATCCAT CATGGCACGA
    GAAACGTGGG AAGTCTTACT ATTGTTTCTT CTGCAGATTA ATGACATACT TCTAGCCCCG
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTTTGGT TACTAGCTTG TACTCGGTGC TTTCCAACAC CTCCTTATTG GAAAACCGCC
    AAGGAGATGG TGGCAAACTG GAGGCACCAC CCGGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ACCTGTGCCC TCACTTCAAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTCCATTT
    AAAGTTCCTG ATGAAGATGC CAGTCTGATC CCTCCTGAAA TGGATAATGA GTGTGTAGCA
    CAGACATGGT TTCGCTTCTT ACATATGTTA AGTAATCCCG TGGATTTGAG TAACCCGGCC
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTAA ATGTGAGCGG AATGCCACAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTAGTGGA TGCATTCTTA GGCATTTCTA GACCCCGATC AGACAGTGCT
    CCTCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGCA CTGCTGTCAG TACCACCCCA
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC GAAAGTTCAG CACCAGACAT CCTCCACCTC TCCTCTGTCA
    AGTCCCAATC AGACTAGTTC TGAACCCCGG CCACTGCCTG CCCCTCGAAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCCA TTACAACACA AGCTAGCATG
    GAGTTTCGAC GGAAAGGGTC ACAAATGTCC ACAGACACCA TGGTTTCCAA TCCTATGTTT
    GATGCAAGTG AATTTCCTGA TAACTATGAA GCAGGAAGAG CTGAGGCTTG TGGGACACTG
    TGTAGGATTT TTTGTAGCAA GAAGACTGGA GAAGAGATTC TGCCAGCTTA TTTATCCAGA
    TTTTACATGC TTTTAATTCA AGGTTTGCAG ATAAATGATT ATGTGTGCCA TCCTGTCTTG
    GCCAGCGTTA TTCTAAACTC CCCTCCTTTG TTCTGCTGTG ACTTGAAAGG GATTGATGTT
    GTGGTTCCTT ACTTTATTTC AGCTCTTGAA ACCATTTTGC CTGACAGAGA ACTCTCAAAA
    TTCAAAAGCT ATGTAAATCC AACAGAATTG AGAAGATCTT CCATTAATAT CCTCCTTTCT
    TTATTGCCCC TCCCTCATCA TTTTGGCACA GTCAGATCTG AGGTGGTCCT GGAAGGGAAG
    TTTAGTAATG ATGACAGCTC TTCATATGAT AAACCAATAA CTTTTTTGTC CCTGAAGTTG
    AGACTTGTGA ATATACTAAT AGGTGCCTTG CAAACGGAAA CGGACCCCAA CAACACTCAA
    ATGATACTAG GGGCAATGTT AAATATTGTT CAAGATTCAG CACTTTTAGA AGCCATTGGT
    TGCCAAATGG AGATGGGTGG TGGAGAAAAT AACCTGAAGA GTCATAGTCG TACTAATAGT
    GGTATTAGTT CAGCAAGTGG TGGAAGCACA GAACCCACAA CTCCTGATAG TGAAAGACCT
    GCTCAAGCTC TCCTAAGGGA TTATGCTCTT AATACAGATT CAGCTGCTGG GCTCCTGATT
    CGCAGCATTC ATCTTGTCAC CCAAAGACTC AACTCTCAGT GGCGGCAAGA CATGAGCATA
    TCACTGGCAG CGCTGGAGCT CCTCTCTGGC CTGGCAAAGG TGAAGGTGAT GGTTGACTCG
    GGAGACCGGA AACGAGCCAT CAGTTCTGTG TGCAGCTACA TCGTGTACCA ATGTAGTCGG
    CCAGCTCCTC TTCACTCCCG CGATCTGCAC TCCATGATAG TAGCAGCTTT TCAGTGTCTC
    TGTGTCTGGC TGACAGAGCA TCCTGATATG CTTGATGAAA AGGACTGTCT TAAGGAAGTA
    TTGGAGATTG TGGAACTGGG TATTTCAGGA AGTAAGTCCA AGAACAGTGA GCAAGAGGTC
    AAGTACAAAG GAGATAAGGA GCCAAACCCT GCATCTATGA GGGTAAAGGA TGCTGCTGAA
    GCCACTCTAA CATGTATTAT GCAGTTGCTC GGCGCGTTTC CTTCACCCAG TGGTCCTGCC
    TCTCCTTGTA GTCTGGTGAA TGAGACCACT TTGATTAAAT ACTCAAGGCT GCCAACCATA
    AACAAGCATA GTTTCCGGTA CTTTGTCTTG GATAACAGTG TCATCCTGGC AATGCTGGAG
    CAACCTCTTG GAAATGAACA GAATGATTTT TTTCCCTCTG TCACTGTGCT GGTTCGAGGA
    ATGTCTGGAA GACTTGCTTG GGCACAACAA CTTTGCCTTT TACCCAGAGG AGCAAAAGCA
    AATCAGAAGC TTTTTGTACC TGAACCTCGT CCAATTCCTA AAAATGATGT TGGATTTAAA
    TATACTGTCA AACATCGACC ATTTCCTGAA GAGGTGGATA AGATTCCTTT TGTGAAAGCA
    GATCTGAGCA TTCCAGATTT GCATGAAATT GTCACTGAAG AATTAGAAGA GAGACATGAA
    AAATTAAGGA ATGGCATGGC CCAGCAGATT GCTTATGAAA TACACCTTGA ACAACAAAGT
    GAGGAAGAAT TGCAGAAGAG AAGTTTTCCT GATCCAATTA CGGATTGTAA GCCTCCACCT
    CCTGCCCAAG AATTCCAAAC AGCACGCCTC TTCCTTTCAC ACTTTGGATT TTTGTCTTTA
    GAAGCATTGA AGGAACCTGC AAATAGTCGC CTACCTCCTC ACCTTATTGC ACTTGACTCC
    ACGATACCTG GATTTTTTGA TGACATTGGA TATCTGGATC TCTTGCCGTG TCGTCCCTTT
    GACACAGTTT TTATTTTCTA TATGAAACCA GGTCAGAAAA CAAACCAGGA GATTTTAAAG
    AATGTGGAAT CTTCCAGAAA TGTTCAGCCA CATTTCCTAG AATTTTTGCT CTCCCTTGGC
    TGGTCAGTAG ATGTGGGCAG ACACCCAGGT TGGACTGGGC ATGTTTCTAC CAGTTGGTCT
    ATTAACAGTT GTGATGATGG TGAAGGACCT GAACAAGATG AAGTGATTTC CTCTGAAGAC
    ATTGGGGCTA GCATTTTCAA TGGACAGAAG AAGGTGCTGT ATTATGCTGA TGCCCTTACG
    GAAATAGCTT TTGTAGTTCC TTCTCCTGTG GAGTCTTTAA CTGATTCATT AGAAAGTAAC
    ATCTCGGACC AAGATAGTGA TTCAAATATG GATCTTATGC CTGGAATTCT GAAACAGCCA
    TCCCTGACAC TTGAGCTTTT CCCTAATCAC ACAGACAATC TTAATTCCTC ACAGCGGCTT
    AGTCCCAGTT CCAGAATGAA GAAGCTGCCT CAGGGTCGCC CTGTGCCTCC CCTTGGACCT
    GAGACAAGAG TTTCTGTAGT CTGGGTGGAA CGCTATGATG ATATAGAGAA CTTTCCCCTC
    TCAGACCTGC TGACAGAGAT CAGTACTGGT GTGGAAACTA CTGCAAACAG CAGCACTTCT
    CTGAGATCTA CCACTCTTGA AAAAGAAGTT CCTGTCATCT TTATCCACCC TTTAAACACT
    GGATTATTCC GGATAAAAAT TCAAGGAGCC ACTGGAAAAT TTAACATGGT CATCCCGCTC
    GTGGATGGGA TGATTGTCAG CAGACGAGCA CTTGGTTTTC TGGTGAGGCA GACTGTAATT
    AATATTTGTA GAAGAAAGAG ACTGGAGAGT GACTCCTACA GTCCACCACA TGTCCGCCGG
    AAACAGAAAA TCACCGACAT TGTCAACAAG TACCGGAACA AGCAGTTGGA GCCAGAGTTT
    TATACTTCAC TTTTCCAGGA GGTTGGACTC AAGAACTGCT CTTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_852604.1
    CDS333..4820
    Translation

    Target ORF information:

    RefSeq Version XM_847511.4
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Ral GTPase activating protein, beta subunit (non-catalytic) (RALGAPB), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_847511.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATCCAGA ATGATCAGGG CCACACGAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGG CGAGAGGTGG CAAATGCAGT GGTCCATCCC
    CTTGGGCAGG CCTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAGTTTGTT AAAAACTGAC
    AAAGAAGTGA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGTGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTATTGCCG
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC TATATGTTCA AAGTATACTA
    AAACACCTGC AAAATCTTTT TGTACCAAGA CAGGAACAGG GCTCCAGTCA GATCCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTAGCCCGTG AGTCATCCAT CATGGCACGA
    GAAACGTGGG AAGTCTTACT ATTGTTTCTT CTGCAGATTA ATGACATACT TCTAGCCCCG
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTTTGGT TACTAGCTTG TACTCGGTGC TTTCCAACAC CTCCTTATTG GAAAACCGCC
    AAGGAGATGG TGGCAAACTG GAGGCACCAC CCGGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ACCTGTGCCC TCACTTCAAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTCCATTT
    AAAGTTCCTG ATGAAGATGC CAGTCTGATC CCTCCTGAAA TGGATAATGA GTGTGTAGCA
    CAGACATGGT TTCGCTTCTT ACATATGTTA AGTAATCCCG TGGATTTGAG TAACCCGGCC
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTAA ATGTGAGCGG AATGCCACAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTAGTGGA TGCATTCTTA GGCATTTCTA GACCCCGATC AGACAGTGCT
    CCTCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGCA CTGCTGTCAG TACCACCCCA
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC GAAAGTTCAG CACCAGACAT CCTCCACCTC TCCTCTGTCA
    AGTCCCAATC AGACTAGTTC TGAACCCCGG CCACTGCCTG CCCCTCGAAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCCA TTACAACACA AGCTAGCATG
    GAGTTTCGAC GGAAAGGGTC ACAAATGTCC ACAGACACCA TGGTTTCCAA TCCTATGTTT
    GATGCAAGTG AATTTCCTGA TAACTATGAA GCAGGAAGAG CTGAGGCTTG TGGGACACTG
    TGTAGGATTT TTTGTAGCAA GAAGACTGGA GAAGAGATTC TGCCAGCTTA TTTATCCAGA
    TTTTACATGC TTTTAATTCA AGGTTTGCAG ATAAATGATT ATGTGTGCCA TCCTGTCTTG
    GCCAGCGTTA TTCTAAACTC CCCTCCTTTG TTCTGCTGTG ACTTGAAAGG GATTGATGTT
    GTGGTTCCTT ACTTTATTTC AGCTCTTGAA ACCATTTTGC CTGACAGAGA ACTCTCAAAA
    TTCAAAAGCT ATGTAAATCC AACAGAATTG AGAAGATCTT CCATTAATAT CCTCCTTTCT
    TTATTGCCCC TCCCTCATCA TTTTGGCACA GTCAGATCTG AGGTGGTCCT GGAAGGGAAG
    TTTAGTAATG ATGACAGCTC TTCATATGAT AAACCAATAA CTTTTTTGTC CCTGAAGTTG
    AGACTTGTGA ATATACTAAT AGGTGCCTTG CAAACGGAAA CGGACCCCAA CAACACTCAA
    ATGATACTAG GGGCAATGTT AAATATTGTT CAAGATTCAG CACTTTTAGA AGCCATTGGT
    TGCCAAATGG AGATGGGTGG TGGAGAAAAT AACCTGAAGA GTCATAGTCG TACTAATAGT
    GGTATTAGTT CAGCAAGTGG TGGAAGCACA GAACCCACAA CTCCTGATAG TGAAAGACCT
    GCTCAAGCTC TCCTAAGGGA TTATGCTCTT AATACAGATT CAGCTGCTGG GCTCCTGATT
    CGCAGCATTC ATCTTGTCAC CCAAAGACTC AACTCTCAGT GGCGGCAAGA CATGAGCATA
    TCACTGGCAG CGCTGGAGCT CCTCTCTGGC CTGGCAAAGG TGAAGGTGAT GGTTGACTCG
    GGAGACCGGA AACGAGCCAT CAGTTCTGTG TGCAGCTACA TCGTGTACCA ATGTAGTCGG
    CCAGCTCCTC TTCACTCCCG CGATCTGCAC TCCATGATAG TAGCAGCTTT TCAGTGTCTC
    TGTGTCTGGC TGACAGAGCA TCCTGATATG CTTGATGAAA AGGACTGTCT TAAGGAAGTA
    TTGGAGATTG TGGAACTGGG TATTTCAGGA AGTAAGTCCA AGAACAGTGA GCAAGAGGTC
    AAGTACAAAG GAGATAAGGA GCCAAACCCT GCATCTATGA GGGTAAAGGA TGCTGCTGAA
    GCCACTCTAA CATGTATTAT GCAGTTGCTC GGCGCGTTTC CTTCACCCAG TGGTCCTGCC
    TCTCCTTGTA GTCTGGTGAA TGAGACCACT TTGATTAAAT ACTCAAGGCT GCCAACCATA
    AACAAGCATA GTTTCCGGTA CTTTGTCTTG GATAACAGTG TCATCCTGGC AATGCTGGAG
    CAACCTCTTG GAAATGAACA GAATGATTTT TTTCCCTCTG TCACTGTGCT GGTTCGAGGA
    ATGTCTGGAA GACTTGCTTG GGCACAACAA CTTTGCCTTT TACCCAGAGG AGCAAAAGCA
    AATCAGAAGC TTTTTGTACC TGAACCTCGT CCAATTCCTA AAAATGATGT TGGATTTAAA
    TATACTGTCA AACATCGACC ATTTCCTGAA GAGGTGGATA AGATTCCTTT TGTGAAAGCA
    GATCTGAGCA TTCCAGATTT GCATGAAATT GTCACTGAAG AATTAGAAGA GAGACATGAA
    AAATTAAGGA ATGGCATGGC CCAGCAGATT GCTTATGAAA TACACCTTGA ACAACAAAGT
    GAGGAAGAAT TGCAGAAGAG AAGTTTTCCT GATCCAATTA CGGATTGTAA GCCTCCACCT
    CCTGCCCAAG AATTCCAAAC AGCACGCCTC TTCCTTTCAC ACTTTGGATT TTTGTCTTTA
    GAAGCATTGA AGGAACCTGC AAATAGTCGC CTACCTCCTC ACCTTATTGC ACTTGACTCC
    ACGATACCTG GATTTTTTGA TGACATTGGA TATCTGGATC TCTTGCCGTG TCGTCCCTTT
    GACACAGTTT TTATTTTCTA TATGAAACCA GGTCAGAAAA CAAACCAGGA GATTTTAAAG
    AATGTGGAAT CTTCCAGAAA TGTTCAGCCA CATTTCCTAG AATTTTTGCT CTCCCTTGGC
    TGGTCAGTAG ATGTGGGCAG ACACCCAGGT TGGACTGGGC ATGTTTCTAC CAGTTGGTCT
    ATTAACAGTT GTGATGATGG TGAAGGACCT GAACAAGATG AAGTGATTTC CTCTGAAGAC
    ATTGGGGCTA GCATTTTCAA TGGACAGAAG AAGGTGCTGT ATTATGCTGA TGCCCTTACG
    GAAATAGCTT TTGTAGTTCC TTCTCCTGTG GAGTCTTTAA CTGATTCATT AGAAAGTAAC
    ATCTCGGACC AAGATAGTGA TTCAAATATG GATCTTATGC CTGGAATTCT GAAACAGCCA
    TCCCTGACAC TTGAGCTTTT CCCTAATCAC ACAGACAATC TTAATTCCTC ACAGCGGCTT
    AGTCCCAGTT CCAGAATGAA GAAGCTGCCT CAGGGTCGCC CTGTGCCTCC CCTTGGACCT
    GAGACAAGAG TTTCTGTAGT CTGGGTGGAA CGCTATGATG ATATAGAGAA CTTTCCCCTC
    TCAGACCTGC TGACAGAGAT CAGTACTGGT GTGGAAACTA CTGCAAACAG CAGCACTTCT
    CTGAGATCTA CCACTCTTGA AAAAGAAGTT CCTGTCATCT TTATCCACCC TTTAAACACT
    GGATTATTCC GGATAAAAAT TCAAGGAGCC ACTGGAAAAT TTAACATGGT CATCCCGCTC
    GTGGATGGGA TGATTGTCAG CAGACGAGCA CTTGGTTTTC TGGTGAGGCA GACTGTAATT
    AATATTTGTA GAAGAAAGAG ACTGGAGAGT GACTCCTACA GTCCACCACA TGTCCGCCGG
    AAACAGAAAA TCACCGACAT TGTCAACAAG TACCGGAACA AGCAGTTGGA GCCAGAGTTT
    TATACTTCAC TTTTCCAGGA GGTTGGACTC AAGAACTGCT CTTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe24894
    Clone ID Related Accession (Same CDS sequence) XM_005635055.2 , XM_022409447.1
    Accession Version XM_005635055.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4476bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ral GTPase-activating protein subunit beta isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726101.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005635055.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATCCAGA ATGATCAGGG CCACACGAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGG CGAGAGGTGG CAAATGCAGT GGTCCATCCC
    CTTGGGCAGG CCTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAGTTTGTT AAAAACTGAC
    AAAGAAGTGA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGTGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTATTGCCG
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC TATATGTTCA AAGTATACTA
    AAACACCTGC AAAATCTTTT TGTACCAAGA CAGGAACAGG GCTCCAGTCA GATCCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTAGCCCGTG AGTCATCCAT CATGGCACGA
    GAAACGTGGG AAGTCTTACT ATTGTTTCTT CTGCAGATTA ATGACATACT TCTAGCCCCG
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTTTGGT TACTAGCTTG TACTCGGTGC TTTCCAACAC CTCCTTATTG GAAAACCGCC
    AAGGAGATGG TGGCAAACTG GAGGCACCAC CCGGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ACCTGTGCCC TCACTTCAAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTCCATTT
    AAAGTTCCTG ATGAAGATGC CAGTCTGATC CCTCCTGAAA TGGATAATGA GTGTGTAGCA
    CAGACATGGT TTCGCTTCTT ACATATGTTA AGTAATCCCG TGGATTTGAG TAACCCGGCC
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTAA ATGTGAGCGG AATGCCACAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTAGTGGA TGCATTCTTA GGCATTTCTA GACCCCGATC AGACAGTGCT
    CCTCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGCA CTGCTGTCAG TACCACCCCA
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC GAAAGTTCAG CACCAGACAT CCTCCACCTC TCCTCTGTCA
    AGTCCCAATC AGACTAGTTC TGAACCCCGG CCACTGCCTG CCCCTCGAAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCCA TTACAACACA AGCTAGCATG
    GAGTTTCGAC GGAAAGGGTC ACAAATGTCC ACAGACACCA TGGTTTCCAA TCCTATGTTT
    GATGCAAGTG AATTTCCTGA TAACTATGAA GCAGGAAGAG CTGAGGCTTG TGGGACACTG
    TGTAGGATTT TTTGTAGCAA GAAGACTGGA GAAGAGATTC TGCCAGCTTA TTTATCCAGA
    TTTTACATGC TTTTAATTCA AGGTTTGCAG ATAAATGATT ATGTGTGCCA TCCTGTCTTG
    GCCAGCGTTA TTCTAAACTC CCCTCCTTTG TTCTGCTGTG ACTTGAAAGG GATTGATGTT
    GTGGTTCCTT ACTTTATTTC AGCTCTTGAA ACCATTTTGC CTGACAGAGA ACTCTCAAAA
    TTCAAAAGCT ATGTAAATCC AACAGAATTG AGAAGATCTT CCATTAATAT CCTCCTTTCT
    TTATTGCCCC TCCCTCATCA TTTTGGCACA GTCAGATCTG AGGTGGTCCT GGAAGGGAAG
    TTTAGTAATG ATGACAGCTC TTCATATGAT AAACCAATAA CTTTTTTGTC CCTGAAGTTG
    AGACTTGTGA ATATACTAAT AGGTGCCTTG CAAACGGAAA CGGACCCCAA CAACACTCAA
    ATGATACTAG GGGCAATGTT AAATATTGTT CAAGATTCAG CACTTTTAGA AGCCATTGGT
    TGCCAAATGG AGATGGGTGG TGGAGAAAAT AACCTGAAGA GTCATAGTCG TACTAATAGT
    GGTATTAGTT CAGCAAGTGG TGGAAGCACA GAACCCACAA CTCCTGATAG TGAAAGACCT
    GCTCAAGCTC TCCTAAGGGA TTATGATTCA GCTGCTGGGC TCCTGATTCG CAGCATTCAT
    CTTGTCACCC AAAGACTCAA CTCTCAGTGG CGGCAAGACA TGAGCATATC ACTGGCAGCG
    CTGGAGCTCC TCTCTGGCCT GGCAAAGGTG AAGGTGATGG TTGACTCGGG AGACCGGAAA
    CGAGCCATCA GTTCTGTGTG CAGCTACATC GTGTACCAAT GTAGTCGGCC AGCTCCTCTT
    CACTCCCGCG ATCTGCACTC CATGATAGTA GCAGCTTTTC AGTGTCTCTG TGTCTGGCTG
    ACAGAGCATC CTGATATGCT TGATGAAAAG GACTGTCTTA AGGAAGTATT GGAGATTGTG
    GAACTGGGTA TTTCAGGAAG TAAGTCCAAG AACAGTGAGC AAGAGGTCAA GTACAAAGGA
    GATAAGGAGC CAAACCCTGC ATCTATGAGG GTAAAGGATG CTGCTGAAGC CACTCTAACA
    TGTATTATGC AGTTGCTCGG CGCGTTTCCT TCACCCAGTG GTCCTGCCTC TCCTTGTAGT
    CTGGTGAATG AGACCACTTT GATTAAATAC TCAAGGCTGC CAACCATAAA CAAGCATAGT
    TTCCGGTACT TTGTCTTGGA TAACAGTGTC ATCCTGGCAA TGCTGGAGCA ACCTCTTGGA
    AATGAACAGA ATGATTTTTT TCCCTCTGTC ACTGTGCTGG TTCGAGGAAT GTCTGGAAGA
    CTTGCTTGGG CACAACAACT TTGCCTTTTA CCCAGAGGAG CAAAAGCAAA TCAGAAGCTT
    TTTGTACCTG AACCTCGTCC AATTCCTAAA AATGATGTTG GATTTAAATA TACTGTCAAA
    CATCGACCAT TTCCTGAAGA GGTGGATAAG ATTCCTTTTG TGAAAGCAGA TCTGAGCATT
    CCAGATTTGC ATGAAATTGT CACTGAAGAA TTAGAAGAGA GACATGAAAA ATTAAGGAAT
    GGCATGGCCC AGCAGATTGC TTATGAAATA CACCTTGAAC AACAAAGTGA GGAAGAATTG
    CAGAAGAGAA GTTTTCCTGA TCCAATTACG GATTGTAAGC CTCCACCTCC TGCCCAAGAA
    TTCCAAACAG CACGCCTCTT CCTTTCACAC TTTGGATTTT TGTCTTTAGA AGCATTGAAG
    GAACCTGCAA ATAGTCGCCT ACCTCCTCAC CTTATTGCAC TTGACTCCAC GATACCTGGA
    TTTTTTGATG ACATTGGATA TCTGGATCTC TTGCCGTGTC GTCCCTTTGA CACAGTTTTT
    ATTTTCTATA TGAAACCAGG TCAGAAAACA AACCAGGAGA TTTTAAAGAA TGTGGAATCT
    TCCAGAAATG TTCAGCCACA TTTCCTAGAA TTTTTGCTCT CCCTTGGCTG GTCAGTAGAT
    GTGGGCAGAC ACCCAGGTTG GACTGGGCAT GTTTCTACCA GTTGGTCTAT TAACAGTTGT
    GATGATGGTG AAGGACCTGA ACAAGATGAA GTGATTTCCT CTGAAGACAT TGGGGCTAGC
    ATTTTCAATG GACAGAAGAA GGTGCTGTAT TATGCTGATG CCCTTACGGA AATAGCTTTT
    GTAGTTCCTT CTCCTGTGGA GTCTTTAACT GATTCATTAG AAAGTAACAT CTCGGACCAA
    GATAGTGATT CAAATATGGA TCTTATGCCT GGAATTCTGA AACAGCCATC CCTGACACTT
    GAGCTTTTCC CTAATCACAC AGACAATCTT AATTCCTCAC AGCGGCTTAG TCCCAGTTCC
    AGAATGAAGA AGCTGCCTCA GGGTCGCCCT GTGCCTCCCC TTGGACCTGA GACAAGAGTT
    TCTGTAGTCT GGGTGGAACG CTATGATGAT ATAGAGAACT TTCCCCTCTC AGACCTGCTG
    ACAGAGATCA GTACTGGTGT GGAAACTACT GCAAACAGCA GCACTTCTCT GAGATCTACC
    ACTCTTGAAA AAGAAGTTCC TGTCATCTTT ATCCACCCTT TAAACACTGG ATTATTCCGG
    ATAAAAATTC AAGGAGCCAC TGGAAAATTT AACATGGTCA TCCCGCTCGT GGATGGGATG
    ATTGTCAGCA GACGAGCACT TGGTTTTCTG GTGAGGCAGA CTGTAATTAA TATTTGTAGA
    AGAAAGAGAC TGGAGAGTGA CTCCTACAGT CCACCACATG TCCGCCGGAA ACAGAAAATC
    ACCGACATTG TCAACAAGTA CCGGAACAAG CAGTTGGAGC CAGAGTTTTA TACTTCACTT
    TTCCAGGAGG TTGGACTCAA GAACTGCTCT TCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005635112.1
    CDS333..4808
    Translation

    Target ORF information:

    RefSeq Version XM_005635055.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Ral GTPase activating protein, beta subunit (non-catalytic) (RALGAPB), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005635055.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATCCAGA ATGATCAGGG CCACACGAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGG CGAGAGGTGG CAAATGCAGT GGTCCATCCC
    CTTGGGCAGG CCTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAGTTTGTT AAAAACTGAC
    AAAGAAGTGA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGTGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTATTGCCG
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC TATATGTTCA AAGTATACTA
    AAACACCTGC AAAATCTTTT TGTACCAAGA CAGGAACAGG GCTCCAGTCA GATCCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTAGCCCGTG AGTCATCCAT CATGGCACGA
    GAAACGTGGG AAGTCTTACT ATTGTTTCTT CTGCAGATTA ATGACATACT TCTAGCCCCG
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTTTGGT TACTAGCTTG TACTCGGTGC TTTCCAACAC CTCCTTATTG GAAAACCGCC
    AAGGAGATGG TGGCAAACTG GAGGCACCAC CCGGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ACCTGTGCCC TCACTTCAAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTCCATTT
    AAAGTTCCTG ATGAAGATGC CAGTCTGATC CCTCCTGAAA TGGATAATGA GTGTGTAGCA
    CAGACATGGT TTCGCTTCTT ACATATGTTA AGTAATCCCG TGGATTTGAG TAACCCGGCC
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTAA ATGTGAGCGG AATGCCACAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTAGTGGA TGCATTCTTA GGCATTTCTA GACCCCGATC AGACAGTGCT
    CCTCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGCA CTGCTGTCAG TACCACCCCA
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC GAAAGTTCAG CACCAGACAT CCTCCACCTC TCCTCTGTCA
    AGTCCCAATC AGACTAGTTC TGAACCCCGG CCACTGCCTG CCCCTCGAAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCCA TTACAACACA AGCTAGCATG
    GAGTTTCGAC GGAAAGGGTC ACAAATGTCC ACAGACACCA TGGTTTCCAA TCCTATGTTT
    GATGCAAGTG AATTTCCTGA TAACTATGAA GCAGGAAGAG CTGAGGCTTG TGGGACACTG
    TGTAGGATTT TTTGTAGCAA GAAGACTGGA GAAGAGATTC TGCCAGCTTA TTTATCCAGA
    TTTTACATGC TTTTAATTCA AGGTTTGCAG ATAAATGATT ATGTGTGCCA TCCTGTCTTG
    GCCAGCGTTA TTCTAAACTC CCCTCCTTTG TTCTGCTGTG ACTTGAAAGG GATTGATGTT
    GTGGTTCCTT ACTTTATTTC AGCTCTTGAA ACCATTTTGC CTGACAGAGA ACTCTCAAAA
    TTCAAAAGCT ATGTAAATCC AACAGAATTG AGAAGATCTT CCATTAATAT CCTCCTTTCT
    TTATTGCCCC TCCCTCATCA TTTTGGCACA GTCAGATCTG AGGTGGTCCT GGAAGGGAAG
    TTTAGTAATG ATGACAGCTC TTCATATGAT AAACCAATAA CTTTTTTGTC CCTGAAGTTG
    AGACTTGTGA ATATACTAAT AGGTGCCTTG CAAACGGAAA CGGACCCCAA CAACACTCAA
    ATGATACTAG GGGCAATGTT AAATATTGTT CAAGATTCAG CACTTTTAGA AGCCATTGGT
    TGCCAAATGG AGATGGGTGG TGGAGAAAAT AACCTGAAGA GTCATAGTCG TACTAATAGT
    GGTATTAGTT CAGCAAGTGG TGGAAGCACA GAACCCACAA CTCCTGATAG TGAAAGACCT
    GCTCAAGCTC TCCTAAGGGA TTATGATTCA GCTGCTGGGC TCCTGATTCG CAGCATTCAT
    CTTGTCACCC AAAGACTCAA CTCTCAGTGG CGGCAAGACA TGAGCATATC ACTGGCAGCG
    CTGGAGCTCC TCTCTGGCCT GGCAAAGGTG AAGGTGATGG TTGACTCGGG AGACCGGAAA
    CGAGCCATCA GTTCTGTGTG CAGCTACATC GTGTACCAAT GTAGTCGGCC AGCTCCTCTT
    CACTCCCGCG ATCTGCACTC CATGATAGTA GCAGCTTTTC AGTGTCTCTG TGTCTGGCTG
    ACAGAGCATC CTGATATGCT TGATGAAAAG GACTGTCTTA AGGAAGTATT GGAGATTGTG
    GAACTGGGTA TTTCAGGAAG TAAGTCCAAG AACAGTGAGC AAGAGGTCAA GTACAAAGGA
    GATAAGGAGC CAAACCCTGC ATCTATGAGG GTAAAGGATG CTGCTGAAGC CACTCTAACA
    TGTATTATGC AGTTGCTCGG CGCGTTTCCT TCACCCAGTG GTCCTGCCTC TCCTTGTAGT
    CTGGTGAATG AGACCACTTT GATTAAATAC TCAAGGCTGC CAACCATAAA CAAGCATAGT
    TTCCGGTACT TTGTCTTGGA TAACAGTGTC ATCCTGGCAA TGCTGGAGCA ACCTCTTGGA
    AATGAACAGA ATGATTTTTT TCCCTCTGTC ACTGTGCTGG TTCGAGGAAT GTCTGGAAGA
    CTTGCTTGGG CACAACAACT TTGCCTTTTA CCCAGAGGAG CAAAAGCAAA TCAGAAGCTT
    TTTGTACCTG AACCTCGTCC AATTCCTAAA AATGATGTTG GATTTAAATA TACTGTCAAA
    CATCGACCAT TTCCTGAAGA GGTGGATAAG ATTCCTTTTG TGAAAGCAGA TCTGAGCATT
    CCAGATTTGC ATGAAATTGT CACTGAAGAA TTAGAAGAGA GACATGAAAA ATTAAGGAAT
    GGCATGGCCC AGCAGATTGC TTATGAAATA CACCTTGAAC AACAAAGTGA GGAAGAATTG
    CAGAAGAGAA GTTTTCCTGA TCCAATTACG GATTGTAAGC CTCCACCTCC TGCCCAAGAA
    TTCCAAACAG CACGCCTCTT CCTTTCACAC TTTGGATTTT TGTCTTTAGA AGCATTGAAG
    GAACCTGCAA ATAGTCGCCT ACCTCCTCAC CTTATTGCAC TTGACTCCAC GATACCTGGA
    TTTTTTGATG ACATTGGATA TCTGGATCTC TTGCCGTGTC GTCCCTTTGA CACAGTTTTT
    ATTTTCTATA TGAAACCAGG TCAGAAAACA AACCAGGAGA TTTTAAAGAA TGTGGAATCT
    TCCAGAAATG TTCAGCCACA TTTCCTAGAA TTTTTGCTCT CCCTTGGCTG GTCAGTAGAT
    GTGGGCAGAC ACCCAGGTTG GACTGGGCAT GTTTCTACCA GTTGGTCTAT TAACAGTTGT
    GATGATGGTG AAGGACCTGA ACAAGATGAA GTGATTTCCT CTGAAGACAT TGGGGCTAGC
    ATTTTCAATG GACAGAAGAA GGTGCTGTAT TATGCTGATG CCCTTACGGA AATAGCTTTT
    GTAGTTCCTT CTCCTGTGGA GTCTTTAACT GATTCATTAG AAAGTAACAT CTCGGACCAA
    GATAGTGATT CAAATATGGA TCTTATGCCT GGAATTCTGA AACAGCCATC CCTGACACTT
    GAGCTTTTCC CTAATCACAC AGACAATCTT AATTCCTCAC AGCGGCTTAG TCCCAGTTCC
    AGAATGAAGA AGCTGCCTCA GGGTCGCCCT GTGCCTCCCC TTGGACCTGA GACAAGAGTT
    TCTGTAGTCT GGGTGGAACG CTATGATGAT ATAGAGAACT TTCCCCTCTC AGACCTGCTG
    ACAGAGATCA GTACTGGTGT GGAAACTACT GCAAACAGCA GCACTTCTCT GAGATCTACC
    ACTCTTGAAA AAGAAGTTCC TGTCATCTTT ATCCACCCTT TAAACACTGG ATTATTCCGG
    ATAAAAATTC AAGGAGCCAC TGGAAAATTT AACATGGTCA TCCCGCTCGT GGATGGGATG
    ATTGTCAGCA GACGAGCACT TGGTTTTCTG GTGAGGCAGA CTGTAATTAA TATTTGTAGA
    AGAAAGAGAC TGGAGAGTGA CTCCTACAGT CCACCACATG TCCGCCGGAA ACAGAAAATC
    ACCGACATTG TCAACAAGTA CCGGAACAAG CAGTTGGAGC CAGAGTTTTA TACTTCACTT
    TTCCAGGAGG TTGGACTCAA GAACTGCTCT TCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe24894
    Clone ID Related Accession (Same CDS sequence) XM_005635055.2 , XM_022409447.1
    Accession Version XM_022409447.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4476bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ral GTPase-activating protein subunit beta isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATCCAGA ATGATCAGGG CCACACGAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGG CGAGAGGTGG CAAATGCAGT GGTCCATCCC
    CTTGGGCAGG CCTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAGTTTGTT AAAAACTGAC
    AAAGAAGTGA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGTGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTATTGCCG
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC TATATGTTCA AAGTATACTA
    AAACACCTGC AAAATCTTTT TGTACCAAGA CAGGAACAGG GCTCCAGTCA GATCCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTAGCCCGTG AGTCATCCAT CATGGCACGA
    GAAACGTGGG AAGTCTTACT ATTGTTTCTT CTGCAGATTA ATGACATACT TCTAGCCCCG
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTTTGGT TACTAGCTTG TACTCGGTGC TTTCCAACAC CTCCTTATTG GAAAACCGCC
    AAGGAGATGG TGGCAAACTG GAGGCACCAC CCGGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ACCTGTGCCC TCACTTCAAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTCCATTT
    AAAGTTCCTG ATGAAGATGC CAGTCTGATC CCTCCTGAAA TGGATAATGA GTGTGTAGCA
    CAGACATGGT TTCGCTTCTT ACATATGTTA AGTAATCCCG TGGATTTGAG TAACCCGGCC
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTAA ATGTGAGCGG AATGCCACAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTAGTGGA TGCATTCTTA GGCATTTCTA GACCCCGATC AGACAGTGCT
    CCTCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGCA CTGCTGTCAG TACCACCCCA
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC GAAAGTTCAG CACCAGACAT CCTCCACCTC TCCTCTGTCA
    AGTCCCAATC AGACTAGTTC TGAACCCCGG CCACTGCCTG CCCCTCGAAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCCA TTACAACACA AGCTAGCATG
    GAGTTTCGAC GGAAAGGGTC ACAAATGTCC ACAGACACCA TGGTTTCCAA TCCTATGTTT
    GATGCAAGTG AATTTCCTGA TAACTATGAA GCAGGAAGAG CTGAGGCTTG TGGGACACTG
    TGTAGGATTT TTTGTAGCAA GAAGACTGGA GAAGAGATTC TGCCAGCTTA TTTATCCAGA
    TTTTACATGC TTTTAATTCA AGGTTTGCAG ATAAATGATT ATGTGTGCCA TCCTGTCTTG
    GCCAGCGTTA TTCTAAACTC CCCTCCTTTG TTCTGCTGTG ACTTGAAAGG GATTGATGTT
    GTGGTTCCTT ACTTTATTTC AGCTCTTGAA ACCATTTTGC CTGACAGAGA ACTCTCAAAA
    TTCAAAAGCT ATGTAAATCC AACAGAATTG AGAAGATCTT CCATTAATAT CCTCCTTTCT
    TTATTGCCCC TCCCTCATCA TTTTGGCACA GTCAGATCTG AGGTGGTCCT GGAAGGGAAG
    TTTAGTAATG ATGACAGCTC TTCATATGAT AAACCAATAA CTTTTTTGTC CCTGAAGTTG
    AGACTTGTGA ATATACTAAT AGGTGCCTTG CAAACGGAAA CGGACCCCAA CAACACTCAA
    ATGATACTAG GGGCAATGTT AAATATTGTT CAAGATTCAG CACTTTTAGA AGCCATTGGT
    TGCCAAATGG AGATGGGTGG TGGAGAAAAT AACCTGAAGA GTCATAGTCG TACTAATAGT
    GGTATTAGTT CAGCAAGTGG TGGAAGCACA GAACCCACAA CTCCTGATAG TGAAAGACCT
    GCTCAAGCTC TCCTAAGGGA TTATGATTCA GCTGCTGGGC TCCTGATTCG CAGCATTCAT
    CTTGTCACCC AAAGACTCAA CTCTCAGTGG CGGCAAGACA TGAGCATATC ACTGGCAGCG
    CTGGAGCTCC TCTCTGGCCT GGCAAAGGTG AAGGTGATGG TTGACTCGGG AGACCGGAAA
    CGAGCCATCA GTTCTGTGTG CAGCTACATC GTGTACCAAT GTAGTCGGCC AGCTCCTCTT
    CACTCCCGCG ATCTGCACTC CATGATAGTA GCAGCTTTTC AGTGTCTCTG TGTCTGGCTG
    ACAGAGCATC CTGATATGCT TGATGAAAAG GACTGTCTTA AGGAAGTATT GGAGATTGTG
    GAACTGGGTA TTTCAGGAAG TAAGTCCAAG AACAGTGAGC AAGAGGTCAA GTACAAAGGA
    GATAAGGAGC CAAACCCTGC ATCTATGAGG GTAAAGGATG CTGCTGAAGC CACTCTAACA
    TGTATTATGC AGTTGCTCGG CGCGTTTCCT TCACCCAGTG GTCCTGCCTC TCCTTGTAGT
    CTGGTGAATG AGACCACTTT GATTAAATAC TCAAGGCTGC CAACCATAAA CAAGCATAGT
    TTCCGGTACT TTGTCTTGGA TAACAGTGTC ATCCTGGCAA TGCTGGAGCA ACCTCTTGGA
    AATGAACAGA ATGATTTTTT TCCCTCTGTC ACTGTGCTGG TTCGAGGAAT GTCTGGAAGA
    CTTGCTTGGG CACAACAACT TTGCCTTTTA CCCAGAGGAG CAAAAGCAAA TCAGAAGCTT
    TTTGTACCTG AACCTCGTCC AATTCCTAAA AATGATGTTG GATTTAAATA TACTGTCAAA
    CATCGACCAT TTCCTGAAGA GGTGGATAAG ATTCCTTTTG TGAAAGCAGA TCTGAGCATT
    CCAGATTTGC ATGAAATTGT CACTGAAGAA TTAGAAGAGA GACATGAAAA ATTAAGGAAT
    GGCATGGCCC AGCAGATTGC TTATGAAATA CACCTTGAAC AACAAAGTGA GGAAGAATTG
    CAGAAGAGAA GTTTTCCTGA TCCAATTACG GATTGTAAGC CTCCACCTCC TGCCCAAGAA
    TTCCAAACAG CACGCCTCTT CCTTTCACAC TTTGGATTTT TGTCTTTAGA AGCATTGAAG
    GAACCTGCAA ATAGTCGCCT ACCTCCTCAC CTTATTGCAC TTGACTCCAC GATACCTGGA
    TTTTTTGATG ACATTGGATA TCTGGATCTC TTGCCGTGTC GTCCCTTTGA CACAGTTTTT
    ATTTTCTATA TGAAACCAGG TCAGAAAACA AACCAGGAGA TTTTAAAGAA TGTGGAATCT
    TCCAGAAATG TTCAGCCACA TTTCCTAGAA TTTTTGCTCT CCCTTGGCTG GTCAGTAGAT
    GTGGGCAGAC ACCCAGGTTG GACTGGGCAT GTTTCTACCA GTTGGTCTAT TAACAGTTGT
    GATGATGGTG AAGGACCTGA ACAAGATGAA GTGATTTCCT CTGAAGACAT TGGGGCTAGC
    ATTTTCAATG GACAGAAGAA GGTGCTGTAT TATGCTGATG CCCTTACGGA AATAGCTTTT
    GTAGTTCCTT CTCCTGTGGA GTCTTTAACT GATTCATTAG AAAGTAACAT CTCGGACCAA
    GATAGTGATT CAAATATGGA TCTTATGCCT GGAATTCTGA AACAGCCATC CCTGACACTT
    GAGCTTTTCC CTAATCACAC AGACAATCTT AATTCCTCAC AGCGGCTTAG TCCCAGTTCC
    AGAATGAAGA AGCTGCCTCA GGGTCGCCCT GTGCCTCCCC TTGGACCTGA GACAAGAGTT
    TCTGTAGTCT GGGTGGAACG CTATGATGAT ATAGAGAACT TTCCCCTCTC AGACCTGCTG
    ACAGAGATCA GTACTGGTGT GGAAACTACT GCAAACAGCA GCACTTCTCT GAGATCTACC
    ACTCTTGAAA AAGAAGTTCC TGTCATCTTT ATCCACCCTT TAAACACTGG ATTATTCCGG
    ATAAAAATTC AAGGAGCCAC TGGAAAATTT AACATGGTCA TCCCGCTCGT GGATGGGATG
    ATTGTCAGCA GACGAGCACT TGGTTTTCTG GTGAGGCAGA CTGTAATTAA TATTTGTAGA
    AGAAAGAGAC TGGAGAGTGA CTCCTACAGT CCACCACATG TCCGCCGGAA ACAGAAAATC
    ACCGACATTG TCAACAAGTA CCGGAACAAG CAGTTGGAGC CAGAGTTTTA TACTTCACTT
    TTCCAGGAGG TTGGACTCAA GAACTGCTCT TCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022265155.1
    CDS367..4842
    Translation

    Target ORF information:

    RefSeq Version XM_022409447.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022409447.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATCCAGA ATGATCAGGG CCACACGAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGG CGAGAGGTGG CAAATGCAGT GGTCCATCCC
    CTTGGGCAGG CCTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAGTTTGTT AAAAACTGAC
    AAAGAAGTGA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGTGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTATTGCCG
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC TATATGTTCA AAGTATACTA
    AAACACCTGC AAAATCTTTT TGTACCAAGA CAGGAACAGG GCTCCAGTCA GATCCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTAGCCCGTG AGTCATCCAT CATGGCACGA
    GAAACGTGGG AAGTCTTACT ATTGTTTCTT CTGCAGATTA ATGACATACT TCTAGCCCCG
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTTTGGT TACTAGCTTG TACTCGGTGC TTTCCAACAC CTCCTTATTG GAAAACCGCC
    AAGGAGATGG TGGCAAACTG GAGGCACCAC CCGGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ACCTGTGCCC TCACTTCAAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTCCATTT
    AAAGTTCCTG ATGAAGATGC CAGTCTGATC CCTCCTGAAA TGGATAATGA GTGTGTAGCA
    CAGACATGGT TTCGCTTCTT ACATATGTTA AGTAATCCCG TGGATTTGAG TAACCCGGCC
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTAA ATGTGAGCGG AATGCCACAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTAGTGGA TGCATTCTTA GGCATTTCTA GACCCCGATC AGACAGTGCT
    CCTCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGCA CTGCTGTCAG TACCACCCCA
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC GAAAGTTCAG CACCAGACAT CCTCCACCTC TCCTCTGTCA
    AGTCCCAATC AGACTAGTTC TGAACCCCGG CCACTGCCTG CCCCTCGAAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCCA TTACAACACA AGCTAGCATG
    GAGTTTCGAC GGAAAGGGTC ACAAATGTCC ACAGACACCA TGGTTTCCAA TCCTATGTTT
    GATGCAAGTG AATTTCCTGA TAACTATGAA GCAGGAAGAG CTGAGGCTTG TGGGACACTG
    TGTAGGATTT TTTGTAGCAA GAAGACTGGA GAAGAGATTC TGCCAGCTTA TTTATCCAGA
    TTTTACATGC TTTTAATTCA AGGTTTGCAG ATAAATGATT ATGTGTGCCA TCCTGTCTTG
    GCCAGCGTTA TTCTAAACTC CCCTCCTTTG TTCTGCTGTG ACTTGAAAGG GATTGATGTT
    GTGGTTCCTT ACTTTATTTC AGCTCTTGAA ACCATTTTGC CTGACAGAGA ACTCTCAAAA
    TTCAAAAGCT ATGTAAATCC AACAGAATTG AGAAGATCTT CCATTAATAT CCTCCTTTCT
    TTATTGCCCC TCCCTCATCA TTTTGGCACA GTCAGATCTG AGGTGGTCCT GGAAGGGAAG
    TTTAGTAATG ATGACAGCTC TTCATATGAT AAACCAATAA CTTTTTTGTC CCTGAAGTTG
    AGACTTGTGA ATATACTAAT AGGTGCCTTG CAAACGGAAA CGGACCCCAA CAACACTCAA
    ATGATACTAG GGGCAATGTT AAATATTGTT CAAGATTCAG CACTTTTAGA AGCCATTGGT
    TGCCAAATGG AGATGGGTGG TGGAGAAAAT AACCTGAAGA GTCATAGTCG TACTAATAGT
    GGTATTAGTT CAGCAAGTGG TGGAAGCACA GAACCCACAA CTCCTGATAG TGAAAGACCT
    GCTCAAGCTC TCCTAAGGGA TTATGATTCA GCTGCTGGGC TCCTGATTCG CAGCATTCAT
    CTTGTCACCC AAAGACTCAA CTCTCAGTGG CGGCAAGACA TGAGCATATC ACTGGCAGCG
    CTGGAGCTCC TCTCTGGCCT GGCAAAGGTG AAGGTGATGG TTGACTCGGG AGACCGGAAA
    CGAGCCATCA GTTCTGTGTG CAGCTACATC GTGTACCAAT GTAGTCGGCC AGCTCCTCTT
    CACTCCCGCG ATCTGCACTC CATGATAGTA GCAGCTTTTC AGTGTCTCTG TGTCTGGCTG
    ACAGAGCATC CTGATATGCT TGATGAAAAG GACTGTCTTA AGGAAGTATT GGAGATTGTG
    GAACTGGGTA TTTCAGGAAG TAAGTCCAAG AACAGTGAGC AAGAGGTCAA GTACAAAGGA
    GATAAGGAGC CAAACCCTGC ATCTATGAGG GTAAAGGATG CTGCTGAAGC CACTCTAACA
    TGTATTATGC AGTTGCTCGG CGCGTTTCCT TCACCCAGTG GTCCTGCCTC TCCTTGTAGT
    CTGGTGAATG AGACCACTTT GATTAAATAC TCAAGGCTGC CAACCATAAA CAAGCATAGT
    TTCCGGTACT TTGTCTTGGA TAACAGTGTC ATCCTGGCAA TGCTGGAGCA ACCTCTTGGA
    AATGAACAGA ATGATTTTTT TCCCTCTGTC ACTGTGCTGG TTCGAGGAAT GTCTGGAAGA
    CTTGCTTGGG CACAACAACT TTGCCTTTTA CCCAGAGGAG CAAAAGCAAA TCAGAAGCTT
    TTTGTACCTG AACCTCGTCC AATTCCTAAA AATGATGTTG GATTTAAATA TACTGTCAAA
    CATCGACCAT TTCCTGAAGA GGTGGATAAG ATTCCTTTTG TGAAAGCAGA TCTGAGCATT
    CCAGATTTGC ATGAAATTGT CACTGAAGAA TTAGAAGAGA GACATGAAAA ATTAAGGAAT
    GGCATGGCCC AGCAGATTGC TTATGAAATA CACCTTGAAC AACAAAGTGA GGAAGAATTG
    CAGAAGAGAA GTTTTCCTGA TCCAATTACG GATTGTAAGC CTCCACCTCC TGCCCAAGAA
    TTCCAAACAG CACGCCTCTT CCTTTCACAC TTTGGATTTT TGTCTTTAGA AGCATTGAAG
    GAACCTGCAA ATAGTCGCCT ACCTCCTCAC CTTATTGCAC TTGACTCCAC GATACCTGGA
    TTTTTTGATG ACATTGGATA TCTGGATCTC TTGCCGTGTC GTCCCTTTGA CACAGTTTTT
    ATTTTCTATA TGAAACCAGG TCAGAAAACA AACCAGGAGA TTTTAAAGAA TGTGGAATCT
    TCCAGAAATG TTCAGCCACA TTTCCTAGAA TTTTTGCTCT CCCTTGGCTG GTCAGTAGAT
    GTGGGCAGAC ACCCAGGTTG GACTGGGCAT GTTTCTACCA GTTGGTCTAT TAACAGTTGT
    GATGATGGTG AAGGACCTGA ACAAGATGAA GTGATTTCCT CTGAAGACAT TGGGGCTAGC
    ATTTTCAATG GACAGAAGAA GGTGCTGTAT TATGCTGATG CCCTTACGGA AATAGCTTTT
    GTAGTTCCTT CTCCTGTGGA GTCTTTAACT GATTCATTAG AAAGTAACAT CTCGGACCAA
    GATAGTGATT CAAATATGGA TCTTATGCCT GGAATTCTGA AACAGCCATC CCTGACACTT
    GAGCTTTTCC CTAATCACAC AGACAATCTT AATTCCTCAC AGCGGCTTAG TCCCAGTTCC
    AGAATGAAGA AGCTGCCTCA GGGTCGCCCT GTGCCTCCCC TTGGACCTGA GACAAGAGTT
    TCTGTAGTCT GGGTGGAACG CTATGATGAT ATAGAGAACT TTCCCCTCTC AGACCTGCTG
    ACAGAGATCA GTACTGGTGT GGAAACTACT GCAAACAGCA GCACTTCTCT GAGATCTACC
    ACTCTTGAAA AAGAAGTTCC TGTCATCTTT ATCCACCCTT TAAACACTGG ATTATTCCGG
    ATAAAAATTC AAGGAGCCAC TGGAAAATTT AACATGGTCA TCCCGCTCGT GGATGGGATG
    ATTGTCAGCA GACGAGCACT TGGTTTTCTG GTGAGGCAGA CTGTAATTAA TATTTGTAGA
    AGAAAGAGAC TGGAGAGTGA CTCCTACAGT CCACCACATG TCCGCCGGAA ACAGAAAATC
    ACCGACATTG TCAACAAGTA CCGGAACAAG CAGTTGGAGC CAGAGTTTTA TACTTCACTT
    TTCCAGGAGG TTGGACTCAA GAACTGCTCT TCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.