RFWD3 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	RFWD3
Entrez Gene ID	610424
Full Name	ring finger and WD repeat domain 3
General protein information	Preferred Names ring finger and WD repeat domain 3 Names E3 ubiquitin-protein ligase RFWD3
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005620698.2	XP_005620755.1	E3 ubiquitin-protein ligase RFWD3 isoform X2
XM_005620698.3	XP_005620755.1	E3 ubiquitin-protein ligase RFWD3 isoform X2
XM_847931.4	XP_853024.2	E3 ubiquitin-protein ligase RFWD3 isoform X1
XM_847931.3	XP_853024.2	E3 ubiquitin-protein ligase RFWD3 isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Rfwd3	XP_006255701.1
	RFWD3	XP_002802615.1
Danio rerio (zebrafish)	LOC100329327	XP_002667008.1
Drosophila melanogaster (fruit fly)	CG13025	NP_001261971.1
Homo sapiens (human)	RFWD3	NP_060594.3
Mus musculus (house mouse)	Rfwd3	NP_666330.2
Pan troglodytes (chimpanzee)	RFWD3	XP_001137409.1
Canis lupus familiaris (dog)	RFWD3	XP_853024.2
Bos taurus (cattle)	RFWD3	XP_005218437.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005643	XP_315661.2
Xenopus tropicalis (tropical clawed frog)	rfwd3	XP_002931710.1
Gallus gallus (chicken)	RFWD3	XP_414051.3

Gene Ontology

The following RFWD3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RFWD3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe07102	XM_847931.4 Latest version!	Canis lupus familiaris ring finger and WD repeat domain 3 (RFWD3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe07103	XM_005620698.3 Latest version!	Canis lupus familiaris ring finger and WD repeat domain 3 (RFWD3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
View Old Accession Versions >>
OCe07103	XM_005620698.2	Canis lupus familiaris ring finger and WD repeat domain 3 (RFWD3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe07102	XM_847931.3	Canis lupus familiaris ring finger and WD repeat domain 3 (RFWD3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RFWD3 ( XM_847931.4 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_853024.2 Canis lupus familiaris ring finger and WD repeat domain 3 (RFWD3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe07102
Clone ID Related Accession (Same CDS sequence)	XM_847931.4 , XM_847931.3
Accession Version	XM_847931.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase RFWD3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_847931.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATGCT CAGGAAGTGG CTGTTGGCCC GGGTCCCGGC GAGCTGTTTC AAAGAAGCGG 
TCGCCTCTGC GGCGATTTGG CAAGCCCGGC GGCGCCCTCC CAGCCCCAGT GATGGCTCAC 
GAAGCAATGG AATATAACCT TCATGGGCAG TTAGACCATC ATGTTGCAGA ACAGCCTGTT 
CCTGCTGAAG TGGTCATCAG CCTAGAGGGA CCACCCGTGT TCCAGGCTCT ACCTGCAGAA 
GTGGTCATCA GCCAAGAGGG ACCACCCGTG CTCCAGGCTC TACCTGCAGA AGTGGTCAGC 
AGCCAAGAGG GACCACCCCT GCCCCAGCCT GCTCCACACG TGTCTGTTGG CCTGACAGAG 
GAAGGGGAGC TGTTGGGAGA AGATCGTGTG GAGAGCATTA ATCCAGGAGC TTCGGAGGAG 
CATAGTCAGC GATCTCGTGT TAACCCCCCT GTCCAAGTGT CTTCTTTGGA TTCGATGAAC 
AGCTTCATCA GTGGGCTGCA GAGACTTCAT GGCATGCTGG AATTCCTGAG ACCTCCATCA 
GACCACAACG TGGGACCAGT GAGATCCAGA AGGATGAGAG GATCTTCACG CAGGTCAAGA 
ACTACAGGGT CACAGAGGAC AGACAGTACC AGGTCCAGAG CACCATTGGA TGCCTACTTT 
CAAGTGAGCC GGACCCAGCC TCATTTGCCA ACCACCTCCC GTGATTCAGA GACCAGAAAT 
CCTGTTTCTG AAGACTTGCA GGTCTCAAGT AGTTCCAATT CTGACAGCGA TAGCTCCACA 
GAGTTTGAAG AAGTTGTTGC CCAGGCAGAG GAGACTAGAG CTGCTGTTTT AGAAGAGCAA 
GTAGAAGGTT CCTCAGCACA GCAAGAAGTT ACCTGTATCA GTGGAGGAGA GACCTTCCCC 
AAACAGTCTC CCCAGAAGAC GGACCCCCTT CTACCTTCTG TTCCTATGGA TGATGAAGAA 
GGGGACACTT GCACAATCTG CTTGGAGCAC TGGACCAATG CTGGGGATCA CCGCCTCTCA 
GCATTACGCT GTGGGCACCT CTTTGGGTAT AAGTGCATTT CTAAGTGGCT AAAGGGACAG 
GCACGAAAAT GCCCCCAGTG CAACAAGAAA GCCAAGCACA GTGACATCGT CGTTCTTTAC 
GCCCGAACCC TAAGAGCTCT GGACACTAGT GAACACGAGC ACATGAAAAG TTCCTTACTA 
AAGGAGCAGA TGCTAAGGAA GCAGGCGGAG TTAGAATCAG CACAGTGCCG GCTCCAGCTT 
CAAGTACTCA CTGACGAATG CAGCAAGCTT CATAGTCGTG TCCAGGACTT GCAGAAACTT 
ATCGTGCAGC ATCGGGAGCA GATTTCACAG CACCCCAGGG GCTCCCAAGC ATGTTTCCCA 
AACTGCCTGC CCTCCAGCCA GAGCCAGCAC AAGTACCATT TCCAGAAGAC CTTCACAGTG 
TCTCAGACAG GAAACTGCCG AATCATGACA TACTGTGACA CTCTGAGCTG CCTGGTGATG 
TCACAGCCTT CCCCTGAGGC CTCCTTCCTG CCAGGCTTTG GCGTTAAGAT GTTGAGTACT 
GCCAACATGA AAAGCAGTCA GTACATTCCC ATGCATGGCA AACAGATCCG AGGACTGGCT 
TTCAGTAGTC GGTCCAAAGG CTTACTACTC TCGGCTTCCC TAGACAGCAC TGTGAAGCTA 
ACCAGCCTAG AGACAAATAC AGTGGTCCAG ACATATAATG CAGGACGTCC TGTCTGGAGC 
TGCTGCTGGT GTCTTGATGA AAGTAATCAT ATCTATGCCG GTCTGGTCAA TGGTTCGATT 
CTGCTGTATG ACCTTCGAAA CACGAGCTCT CATATCCAGG AGTTAGCACC CCAGAAAGCC 
AGATGCCCGC TGGTGTCCCT ATCCTACATA CCCAGAGCTG CCTCATCTGC ATTTCCATAT 
GGTGGGGTGT TGGCTGGAAC TTTGGAGAAC GCTTCCTTCT GGGAGCTAAA AATGGGCTTT 
TCTCATTGGC CTCATACGCT GCCCATGGAG CCAGGGGGCT ATGTAGACTT TCAGACAGAG 
AGCAGCACAC GGCACTGTCT TGTGACCTAC AGGCCCGATA AAAATCACAG CACTTTACGA 
AGTGTGCTGA TGGAGATGTC CTACAAGCTG GATGATGCTG GAGAGCCAGT CTGTATCTGC 
CATCCTGTAC AAACGTTCCT TGGGGGACCC ACTTGCAAAC TACTGACCAA AAGTGCCATT 
TTCCAAAACC CAGAGAATGA TGGCAGCATC CTGGTGTGTA CTGGGGACGA AGCATCAAAT 
TCTGCCTTGC TGTGGGATGC TGGCAGTGGC TCGTTGCTGC AGGAACTGCA GGCTGGCCAG 
CCTGTCTTGG ACATCTGCCC ATTTGAGGTG AACCACAGTA GCTACTTGGC TACCTTAACA 
GAGAAGGTGG TCCACATCTA TAGGTGGGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_853024.2
CDS	47..2479
Translation	MRCSGSGCWPGSRRAVSKKRSPLRRFGKPGGALPAPVMAHEAMEYNLHGQLDHHVAEQPVPAEVVISLEGPPVFQALPAEVVISQEGPPVLQALPAEVVSSQEGPPLPQPAPHVSVGLTEEGELLGEDRVESINPGASEEHSQRSRVNPPVQVSSLDSMNSFISGLQRLHGMLEFLRPPSDHNVGPVRSRRMRGSSRRSRTTGSQRTDSTRSRAPLDAYFQVSRTQPHLPTTSRDSETRNPVSEDLQVSSSSNSDSDSSTEFEEVVAQAEETRAAVLEEQVEGSSAQQEVTCISGGETFPKQSPQKTDPLLPSVPMDDEEGDTCTICLEHWTNAGDHRLSALRCGHLFGYKCISKWLKGQARKCPQCNKKAKHSDIVVLYARTLRALDTSEHEHMKSSLLKEQMLRKQAELESAQCRLQLQVLTDECSKLHSRVQDLQKLIVQHREQISQHPRGSQACFPNCLPSSQSQHKYHFQKTFTVSQTGNCRIMTYCDTLSCLVMSQPSPEASFLPGFGVKMLSTANMKSSQYIPMHGKQIRGLAFSSRSKGLLLSASLDSTVKLTSLETNTVVQTYNAGRPVWSCCWCLDESNHIYAGLVNGSILLYDLRNTSSHIQELAPQKARCPLVSLSYIPRAASSAFPYGGVLAGTLENASFWELKMGFSHWPHTLPMEPGGYVDFQTESSTRHCLVTYRPDKNHSTLRSVLMEMSYKLDDAGEPVCICHPVQTFLGGPTCKLLTKSAIFQNPENDGSILVCTGDEASNSALLWDAGSGSLLQELQAGQPVLDICPFEVNHSSYLATLTEKVVHIYRWE

Target ORF information:

RefSeq Version	XM_847931.4
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ring finger and WD repeat domain 3 (RFWD3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_847931.4

ORF Insert Sequence:

ATGAGATGCT CAGGAAGTGG CTGTTGGCCC GGGTCCCGGC GAGCTGTTTC AAAGAAGCGG 
TCGCCTCTGC GGCGATTTGG CAAGCCCGGC GGCGCCCTCC CAGCCCCAGT GATGGCTCAC 
GAAGCAATGG AATATAACCT TCATGGGCAG TTAGACCATC ATGTTGCAGA ACAGCCTGTT 
CCTGCTGAAG TGGTCATCAG CCTAGAGGGA CCACCCGTGT TCCAGGCTCT ACCTGCAGAA 
GTGGTCATCA GCCAAGAGGG ACCACCCGTG CTCCAGGCTC TACCTGCAGA AGTGGTCAGC 
AGCCAAGAGG GACCACCCCT GCCCCAGCCT GCTCCACACG TGTCTGTTGG CCTGACAGAG 
GAAGGGGAGC TGTTGGGAGA AGATCGTGTG GAGAGCATTA ATCCAGGAGC TTCGGAGGAG 
CATAGTCAGC GATCTCGTGT TAACCCCCCT GTCCAAGTGT CTTCTTTGGA TTCGATGAAC 
AGCTTCATCA GTGGGCTGCA GAGACTTCAT GGCATGCTGG AATTCCTGAG ACCTCCATCA 
GACCACAACG TGGGACCAGT GAGATCCAGA AGGATGAGAG GATCTTCACG CAGGTCAAGA 
ACTACAGGGT CACAGAGGAC AGACAGTACC AGGTCCAGAG CACCATTGGA TGCCTACTTT 
CAAGTGAGCC GGACCCAGCC TCATTTGCCA ACCACCTCCC GTGATTCAGA GACCAGAAAT 
CCTGTTTCTG AAGACTTGCA GGTCTCAAGT AGTTCCAATT CTGACAGCGA TAGCTCCACA 
GAGTTTGAAG AAGTTGTTGC CCAGGCAGAG GAGACTAGAG CTGCTGTTTT AGAAGAGCAA 
GTAGAAGGTT CCTCAGCACA GCAAGAAGTT ACCTGTATCA GTGGAGGAGA GACCTTCCCC 
AAACAGTCTC CCCAGAAGAC GGACCCCCTT CTACCTTCTG TTCCTATGGA TGATGAAGAA 
GGGGACACTT GCACAATCTG CTTGGAGCAC TGGACCAATG CTGGGGATCA CCGCCTCTCA 
GCATTACGCT GTGGGCACCT CTTTGGGTAT AAGTGCATTT CTAAGTGGCT AAAGGGACAG 
GCACGAAAAT GCCCCCAGTG CAACAAGAAA GCCAAGCACA GTGACATCGT CGTTCTTTAC 
GCCCGAACCC TAAGAGCTCT GGACACTAGT GAACACGAGC ACATGAAAAG TTCCTTACTA 
AAGGAGCAGA TGCTAAGGAA GCAGGCGGAG TTAGAATCAG CACAGTGCCG GCTCCAGCTT 
CAAGTACTCA CTGACGAATG CAGCAAGCTT CATAGTCGTG TCCAGGACTT GCAGAAACTT 
ATCGTGCAGC ATCGGGAGCA GATTTCACAG CACCCCAGGG GCTCCCAAGC ATGTTTCCCA 
AACTGCCTGC CCTCCAGCCA GAGCCAGCAC AAGTACCATT TCCAGAAGAC CTTCACAGTG 
TCTCAGACAG GAAACTGCCG AATCATGACA TACTGTGACA CTCTGAGCTG CCTGGTGATG 
TCACAGCCTT CCCCTGAGGC CTCCTTCCTG CCAGGCTTTG GCGTTAAGAT GTTGAGTACT 
GCCAACATGA AAAGCAGTCA GTACATTCCC ATGCATGGCA AACAGATCCG AGGACTGGCT 
TTCAGTAGTC GGTCCAAAGG CTTACTACTC TCGGCTTCCC TAGACAGCAC TGTGAAGCTA 
ACCAGCCTAG AGACAAATAC AGTGGTCCAG ACATATAATG CAGGACGTCC TGTCTGGAGC 
TGCTGCTGGT GTCTTGATGA AAGTAATCAT ATCTATGCCG GTCTGGTCAA TGGTTCGATT 
CTGCTGTATG ACCTTCGAAA CACGAGCTCT CATATCCAGG AGTTAGCACC CCAGAAAGCC 
AGATGCCCGC TGGTGTCCCT ATCCTACATA CCCAGAGCTG CCTCATCTGC ATTTCCATAT 
GGTGGGGTGT TGGCTGGAAC TTTGGAGAAC GCTTCCTTCT GGGAGCTAAA AATGGGCTTT 
TCTCATTGGC CTCATACGCT GCCCATGGAG CCAGGGGGCT ATGTAGACTT TCAGACAGAG 
AGCAGCACAC GGCACTGTCT TGTGACCTAC AGGCCCGATA AAAATCACAG CACTTTACGA 
AGTGTGCTGA TGGAGATGTC CTACAAGCTG GATGATGCTG GAGAGCCAGT CTGTATCTGC 
CATCCTGTAC AAACGTTCCT TGGGGGACCC ACTTGCAAAC TACTGACCAA AAGTGCCATT 
TTCCAAAACC CAGAGAATGA TGGCAGCATC CTGGTGTGTA CTGGGGACGA AGCATCAAAT 
TCTGCCTTGC TGTGGGATGC TGGCAGTGGC TCGTTGCTGC AGGAACTGCA GGCTGGCCAG 
CCTGTCTTGG ACATCTGCCC ATTTGAGGTG AACCACAGTA GCTACTTGGC TACCTTAACA 
GAGAAGGTGG TCCACATCTA TAGGTGGGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RFWD3 ( XM_847931.3 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_853024.2 Canis lupus familiaris ring finger and WD repeat domain 3 (RFWD3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe07102
Clone ID Related Accession (Same CDS sequence)	XM_847931.4 , XM_847931.3
Accession Version	XM_847931.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase RFWD3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726060.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_847931.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATGCT CAGGAAGTGG CTGTTGGCCC GGGTCCCGGC GAGCTGTTTC AAAGAAGCGG 
TCGCCTCTGC GGCGATTTGG CAAGCCCGGC GGCGCCCTCC CAGCCCCAGT GATGGCTCAC 
GAAGCAATGG AATATAACCT TCATGGGCAG TTAGACCATC ATGTTGCAGA ACAGCCTGTT 
CCTGCTGAAG TGGTCATCAG CCTAGAGGGA CCACCCGTGT TCCAGGCTCT ACCTGCAGAA 
GTGGTCATCA GCCAAGAGGG ACCACCCGTG CTCCAGGCTC TACCTGCAGA AGTGGTCAGC 
AGCCAAGAGG GACCACCCCT GCCCCAGCCT GCTCCACACG TGTCTGTTGG CCTGACAGAG 
GAAGGGGAGC TGTTGGGAGA AGATCGTGTG GAGAGCATTA ATCCAGGAGC TTCGGAGGAG 
CATAGTCAGC GATCTCGTGT TAACCCCCCT GTCCAAGTGT CTTCTTTGGA TTCGATGAAC 
AGCTTCATCA GTGGGCTGCA GAGACTTCAT GGCATGCTGG AATTCCTGAG ACCTCCATCA 
GACCACAACG TGGGACCAGT GAGATCCAGA AGGATGAGAG GATCTTCACG CAGGTCAAGA 
ACTACAGGGT CACAGAGGAC AGACAGTACC AGGTCCAGAG CACCATTGGA TGCCTACTTT 
CAAGTGAGCC GGACCCAGCC TCATTTGCCA ACCACCTCCC GTGATTCAGA GACCAGAAAT 
CCTGTTTCTG AAGACTTGCA GGTCTCAAGT AGTTCCAATT CTGACAGCGA TAGCTCCACA 
GAGTTTGAAG AAGTTGTTGC CCAGGCAGAG GAGACTAGAG CTGCTGTTTT AGAAGAGCAA 
GTAGAAGGTT CCTCAGCACA GCAAGAAGTT ACCTGTATCA GTGGAGGAGA GACCTTCCCC 
AAACAGTCTC CCCAGAAGAC GGACCCCCTT CTACCTTCTG TTCCTATGGA TGATGAAGAA 
GGGGACACTT GCACAATCTG CTTGGAGCAC TGGACCAATG CTGGGGATCA CCGCCTCTCA 
GCATTACGCT GTGGGCACCT CTTTGGGTAT AAGTGCATTT CTAAGTGGCT AAAGGGACAG 
GCACGAAAAT GCCCCCAGTG CAACAAGAAA GCCAAGCACA GTGACATCGT CGTTCTTTAC 
GCCCGAACCC TAAGAGCTCT GGACACTAGT GAACACGAGC ACATGAAAAG TTCCTTACTA 
AAGGAGCAGA TGCTAAGGAA GCAGGCGGAG TTAGAATCAG CACAGTGCCG GCTCCAGCTT 
CAAGTACTCA CTGACGAATG CAGCAAGCTT CATAGTCGTG TCCAGGACTT GCAGAAACTT 
ATCGTGCAGC ATCGGGAGCA GATTTCACAG CACCCCAGGG GCTCCCAAGC ATGTTTCCCA 
AACTGCCTGC CCTCCAGCCA GAGCCAGCAC AAGTACCATT TCCAGAAGAC CTTCACAGTG 
TCTCAGACAG GAAACTGCCG AATCATGACA TACTGTGACA CTCTGAGCTG CCTGGTGATG 
TCACAGCCTT CCCCTGAGGC CTCCTTCCTG CCAGGCTTTG GCGTTAAGAT GTTGAGTACT 
GCCAACATGA AAAGCAGTCA GTACATTCCC ATGCATGGCA AACAGATCCG AGGACTGGCT 
TTCAGTAGTC GGTCCAAAGG CTTACTACTC TCGGCTTCCC TAGACAGCAC TGTGAAGCTA 
ACCAGCCTAG AGACAAATAC AGTGGTCCAG ACATATAATG CAGGACGTCC TGTCTGGAGC 
TGCTGCTGGT GTCTTGATGA AAGTAATCAT ATCTATGCCG GTCTGGTCAA TGGTTCGATT 
CTGCTGTATG ACCTTCGAAA CACGAGCTCT CATATCCAGG AGTTAGCACC CCAGAAAGCC 
AGATGCCCGC TGGTGTCCCT ATCCTACATA CCCAGAGCTG CCTCATCTGC ATTTCCATAT 
GGTGGGGTGT TGGCTGGAAC TTTGGAGAAC GCTTCCTTCT GGGAGCTAAA AATGGGCTTT 
TCTCATTGGC CTCATACGCT GCCCATGGAG CCAGGGGGCT ATGTAGACTT TCAGACAGAG 
AGCAGCACAC GGCACTGTCT TGTGACCTAC AGGCCCGATA AAAATCACAG CACTTTACGA 
AGTGTGCTGA TGGAGATGTC CTACAAGCTG GATGATGCTG GAGAGCCAGT CTGTATCTGC 
CATCCTGTAC AAACGTTCCT TGGGGGACCC ACTTGCAAAC TACTGACCAA AAGTGCCATT 
TTCCAAAACC CAGAGAATGA TGGCAGCATC CTGGTGTGTA CTGGGGACGA AGCATCAAAT 
TCTGCCTTGC TGTGGGATGC TGGCAGTGGC TCGTTGCTGC AGGAACTGCA GGCTGGCCAG 
CCTGTCTTGG ACATCTGCCC ATTTGAGGTG AACCACAGTA GCTACTTGGC TACCTTAACA 
GAGAAGGTGG TCCACATCTA TAGGTGGGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_853024.2
CDS	1752..4184
Translation	MRCSGSGCWPGSRRAVSKKRSPLRRFGKPGGALPAPVMAHEAMEYNLHGQLDHHVAEQPVPAEVVISLEGPPVFQALPAEVVISQEGPPVLQALPAEVVSSQEGPPLPQPAPHVSVGLTEEGELLGEDRVESINPGASEEHSQRSRVNPPVQVSSLDSMNSFISGLQRLHGMLEFLRPPSDHNVGPVRSRRMRGSSRRSRTTGSQRTDSTRSRAPLDAYFQVSRTQPHLPTTSRDSETRNPVSEDLQVSSSSNSDSDSSTEFEEVVAQAEETRAAVLEEQVEGSSAQQEVTCISGGETFPKQSPQKTDPLLPSVPMDDEEGDTCTICLEHWTNAGDHRLSALRCGHLFGYKCISKWLKGQARKCPQCNKKAKHSDIVVLYARTLRALDTSEHEHMKSSLLKEQMLRKQAELESAQCRLQLQVLTDECSKLHSRVQDLQKLIVQHREQISQHPRGSQACFPNCLPSSQSQHKYHFQKTFTVSQTGNCRIMTYCDTLSCLVMSQPSPEASFLPGFGVKMLSTANMKSSQYIPMHGKQIRGLAFSSRSKGLLLSASLDSTVKLTSLETNTVVQTYNAGRPVWSCCWCLDESNHIYAGLVNGSILLYDLRNTSSHIQELAPQKARCPLVSLSYIPRAASSAFPYGGVLAGTLENASFWELKMGFSHWPHTLPMEPGGYVDFQTESSTRHCLVTYRPDKNHSTLRSVLMEMSYKLDDAGEPVCICHPVQTFLGGPTCKLLTKSAIFQNPENDGSILVCTGDEASNSALLWDAGSGSLLQELQAGQPVLDICPFEVNHSSYLATLTEKVVHIYRWE

Target ORF information:

RefSeq Version	XM_847931.3
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ring finger and WD repeat domain 3 (RFWD3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_847931.3

ORF Insert Sequence:

ATGAGATGCT CAGGAAGTGG CTGTTGGCCC GGGTCCCGGC GAGCTGTTTC AAAGAAGCGG 
TCGCCTCTGC GGCGATTTGG CAAGCCCGGC GGCGCCCTCC CAGCCCCAGT GATGGCTCAC 
GAAGCAATGG AATATAACCT TCATGGGCAG TTAGACCATC ATGTTGCAGA ACAGCCTGTT 
CCTGCTGAAG TGGTCATCAG CCTAGAGGGA CCACCCGTGT TCCAGGCTCT ACCTGCAGAA 
GTGGTCATCA GCCAAGAGGG ACCACCCGTG CTCCAGGCTC TACCTGCAGA AGTGGTCAGC 
AGCCAAGAGG GACCACCCCT GCCCCAGCCT GCTCCACACG TGTCTGTTGG CCTGACAGAG 
GAAGGGGAGC TGTTGGGAGA AGATCGTGTG GAGAGCATTA ATCCAGGAGC TTCGGAGGAG 
CATAGTCAGC GATCTCGTGT TAACCCCCCT GTCCAAGTGT CTTCTTTGGA TTCGATGAAC 
AGCTTCATCA GTGGGCTGCA GAGACTTCAT GGCATGCTGG AATTCCTGAG ACCTCCATCA 
GACCACAACG TGGGACCAGT GAGATCCAGA AGGATGAGAG GATCTTCACG CAGGTCAAGA 
ACTACAGGGT CACAGAGGAC AGACAGTACC AGGTCCAGAG CACCATTGGA TGCCTACTTT 
CAAGTGAGCC GGACCCAGCC TCATTTGCCA ACCACCTCCC GTGATTCAGA GACCAGAAAT 
CCTGTTTCTG AAGACTTGCA GGTCTCAAGT AGTTCCAATT CTGACAGCGA TAGCTCCACA 
GAGTTTGAAG AAGTTGTTGC CCAGGCAGAG GAGACTAGAG CTGCTGTTTT AGAAGAGCAA 
GTAGAAGGTT CCTCAGCACA GCAAGAAGTT ACCTGTATCA GTGGAGGAGA GACCTTCCCC 
AAACAGTCTC CCCAGAAGAC GGACCCCCTT CTACCTTCTG TTCCTATGGA TGATGAAGAA 
GGGGACACTT GCACAATCTG CTTGGAGCAC TGGACCAATG CTGGGGATCA CCGCCTCTCA 
GCATTACGCT GTGGGCACCT CTTTGGGTAT AAGTGCATTT CTAAGTGGCT AAAGGGACAG 
GCACGAAAAT GCCCCCAGTG CAACAAGAAA GCCAAGCACA GTGACATCGT CGTTCTTTAC 
GCCCGAACCC TAAGAGCTCT GGACACTAGT GAACACGAGC ACATGAAAAG TTCCTTACTA 
AAGGAGCAGA TGCTAAGGAA GCAGGCGGAG TTAGAATCAG CACAGTGCCG GCTCCAGCTT 
CAAGTACTCA CTGACGAATG CAGCAAGCTT CATAGTCGTG TCCAGGACTT GCAGAAACTT 
ATCGTGCAGC ATCGGGAGCA GATTTCACAG CACCCCAGGG GCTCCCAAGC ATGTTTCCCA 
AACTGCCTGC CCTCCAGCCA GAGCCAGCAC AAGTACCATT TCCAGAAGAC CTTCACAGTG 
TCTCAGACAG GAAACTGCCG AATCATGACA TACTGTGACA CTCTGAGCTG CCTGGTGATG 
TCACAGCCTT CCCCTGAGGC CTCCTTCCTG CCAGGCTTTG GCGTTAAGAT GTTGAGTACT 
GCCAACATGA AAAGCAGTCA GTACATTCCC ATGCATGGCA AACAGATCCG AGGACTGGCT 
TTCAGTAGTC GGTCCAAAGG CTTACTACTC TCGGCTTCCC TAGACAGCAC TGTGAAGCTA 
ACCAGCCTAG AGACAAATAC AGTGGTCCAG ACATATAATG CAGGACGTCC TGTCTGGAGC 
TGCTGCTGGT GTCTTGATGA AAGTAATCAT ATCTATGCCG GTCTGGTCAA TGGTTCGATT 
CTGCTGTATG ACCTTCGAAA CACGAGCTCT CATATCCAGG AGTTAGCACC CCAGAAAGCC 
AGATGCCCGC TGGTGTCCCT ATCCTACATA CCCAGAGCTG CCTCATCTGC ATTTCCATAT 
GGTGGGGTGT TGGCTGGAAC TTTGGAGAAC GCTTCCTTCT GGGAGCTAAA AATGGGCTTT 
TCTCATTGGC CTCATACGCT GCCCATGGAG CCAGGGGGCT ATGTAGACTT TCAGACAGAG 
AGCAGCACAC GGCACTGTCT TGTGACCTAC AGGCCCGATA AAAATCACAG CACTTTACGA 
AGTGTGCTGA TGGAGATGTC CTACAAGCTG GATGATGCTG GAGAGCCAGT CTGTATCTGC 
CATCCTGTAC AAACGTTCCT TGGGGGACCC ACTTGCAAAC TACTGACCAA AAGTGCCATT 
TTCCAAAACC CAGAGAATGA TGGCAGCATC CTGGTGTGTA CTGGGGACGA AGCATCAAAT 
TCTGCCTTGC TGTGGGATGC TGGCAGTGGC TCGTTGCTGC AGGAACTGCA GGCTGGCCAG 
CCTGTCTTGG ACATCTGCCC ATTTGAGGTG AACCACAGTA GCTACTTGGC TACCTTAACA 
GAGAAGGTGG TCCACATCTA TAGGTGGGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RFWD3 ( XM_005620698.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005620755.1 Canis lupus familiaris ring finger and WD repeat domain 3 (RFWD3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe07103
Clone ID Related Accession (Same CDS sequence)	XM_005620698.2 , XM_005620698.3
Accession Version	XM_005620698.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase RFWD3 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726060.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005620698.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAGAG CGGCCAGGAA ACCCGCCCAA GTGATGGCTC ACGAAGCAAT GGAATATAAC 
CTTCATGGGC AGTTAGACCA TCATGTTGCA GAACAGCCTG TTCCTGCTGA AGTGGTCATC 
AGCCTAGAGG GACCACCCGT GTTCCAGGCT CTACCTGCAG AAGTGGTCAT CAGCCAAGAG 
GGACCACCCG TGCTCCAGGC TCTACCTGCA GAAGTGGTCA GCAGCCAAGA GGGACCACCC 
CTGCCCCAGC CTGCTCCACA CGTGTCTGTT GGCCTGACAG AGGAAGGGGA GCTGTTGGGA 
GAAGATCGTG TGGAGAGCAT TAATCCAGGA GCTTCGGAGG AGCATAGTCA GCGATCTCGT 
GTTAACCCCC CTGTCCAAGT GTCTTCTTTG GATTCGATGA ACAGCTTCAT CAGTGGGCTG 
CAGAGACTTC ATGGCATGCT GGAATTCCTG AGACCTCCAT CAGACCACAA CGTGGGACCA 
GTGAGATCCA GAAGGATGAG AGGATCTTCA CGCAGGTCAA GAACTACAGG GTCACAGAGG 
ACAGACAGTA CCAGGTCCAG AGCACCATTG GATGCCTACT TTCAAGTGAG CCGGACCCAG 
CCTCATTTGC CAACCACCTC CCGTGATTCA GAGACCAGAA ATCCTGTTTC TGAAGACTTG 
CAGGTCTCAA GTAGTTCCAA TTCTGACAGC GATAGCTCCA CAGAGTTTGA AGAAGTTGTT 
GCCCAGGCAG AGGAGACTAG AGCTGCTGTT TTAGAAGAGC AAGTAGAAGG TTCCTCAGCA 
CAGCAAGAAG TTACCTGTAT CAGTGGAGGA GAGACCTTCC CCAAACAGTC TCCCCAGAAG 
ACGGACCCCC TTCTACCTTC TGTTCCTATG GATGATGAAG AAGGGGACAC TTGCACAATC 
TGCTTGGAGC ACTGGACCAA TGCTGGGGAT CACCGCCTCT CAGCATTACG CTGTGGGCAC 
CTCTTTGGGT ATAAGTGCAT TTCTAAGTGG CTAAAGGGAC AGGCACGAAA ATGCCCCCAG 
TGCAACAAGA AAGCCAAGCA CAGTGACATC GTCGTTCTTT ACGCCCGAAC CCTAAGAGCT 
CTGGACACTA GTGAACACGA GCACATGAAA AGTTCCTTAC TAAAGGAGCA GATGCTAAGG 
AAGCAGGCGG AGTTAGAATC AGCACAGTGC CGGCTCCAGC TTCAAGTACT CACTGACGAA 
TGCAGCAAGC TTCATAGTCG TGTCCAGGAC TTGCAGAAAC TTATCGTGCA GCATCGGGAG 
CAGATTTCAC AGCACCCCAG GGGCTCCCAA GCATGTTTCC CAAACTGCCT GCCCTCCAGC 
CAGAGCCAGC ACAAGTACCA TTTCCAGAAG ACCTTCACAG TGTCTCAGAC AGGAAACTGC 
CGAATCATGA CATACTGTGA CACTCTGAGC TGCCTGGTGA TGTCACAGCC TTCCCCTGAG 
GCCTCCTTCC TGCCAGGCTT TGGCGTTAAG ATGTTGAGTA CTGCCAACAT GAAAAGCAGT 
CAGTACATTC CCATGCATGG CAAACAGATC CGAGGACTGG CTTTCAGTAG TCGGTCCAAA 
GGCTTACTAC TCTCGGCTTC CCTAGACAGC ACTGTGAAGC TAACCAGCCT AGAGACAAAT 
ACAGTGGTCC AGACATATAA TGCAGGACGT CCTGTCTGGA GCTGCTGCTG GTGTCTTGAT 
GAAAGTAATC ATATCTATGC CGGTCTGGTC AATGGTTCGA TTCTGCTGTA TGACCTTCGA 
AACACGAGCT CTCATATCCA GGAGTTAGCA CCCCAGAAAG CCAGATGCCC GCTGGTGTCC 
CTATCCTACA TACCCAGAGC TGCCTCATCT GCATTTCCAT ATGGTGGGGT GTTGGCTGGA 
ACTTTGGAGA ACGCTTCCTT CTGGGAGCTA AAAATGGGCT TTTCTCATTG GCCTCATACG 
CTGCCCATGG AGCCAGGGGG CTATGTAGAC TTTCAGACAG AGAGCAGCAC ACGGCACTGT 
CTTGTGACCT ACAGGCCCGA TAAAAATCAC AGCACTTTAC GAAGTGTGCT GATGGAGATG 
TCCTACAAGC TGGATGATGC TGGAGAGCCA GTCTGTATCT GCCATCCTGT ACAAACGTTC 
CTTGGGGGAC CCACTTGCAA ACTACTGACC AAAAGTGCCA TTTTCCAAAA CCCAGAGAAT 
GATGGCAGCA TCCTGGTGTG TACTGGGGAC GAAGCATCAA ATTCTGCCTT GCTGTGGGAT 
GCTGGCAGTG GCTCGTTGCT GCAGGAACTG CAGGCTGGCC AGCCTGTCTT GGACATCTGC 
CCATTTGAGG TGAACCACAG TAGCTACTTG GCTACCTTAA CAGAGAAGGT GGTCCACATC 
TATAGGTGGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620755.1
CDS	517..2871
Translation	MNRAARKPAQVMAHEAMEYNLHGQLDHHVAEQPVPAEVVISLEGPPVFQALPAEVVISQEGPPVLQALPAEVVSSQEGPPLPQPAPHVSVGLTEEGELLGEDRVESINPGASEEHSQRSRVNPPVQVSSLDSMNSFISGLQRLHGMLEFLRPPSDHNVGPVRSRRMRGSSRRSRTTGSQRTDSTRSRAPLDAYFQVSRTQPHLPTTSRDSETRNPVSEDLQVSSSSNSDSDSSTEFEEVVAQAEETRAAVLEEQVEGSSAQQEVTCISGGETFPKQSPQKTDPLLPSVPMDDEEGDTCTICLEHWTNAGDHRLSALRCGHLFGYKCISKWLKGQARKCPQCNKKAKHSDIVVLYARTLRALDTSEHEHMKSSLLKEQMLRKQAELESAQCRLQLQVLTDECSKLHSRVQDLQKLIVQHREQISQHPRGSQACFPNCLPSSQSQHKYHFQKTFTVSQTGNCRIMTYCDTLSCLVMSQPSPEASFLPGFGVKMLSTANMKSSQYIPMHGKQIRGLAFSSRSKGLLLSASLDSTVKLTSLETNTVVQTYNAGRPVWSCCWCLDESNHIYAGLVNGSILLYDLRNTSSHIQELAPQKARCPLVSLSYIPRAASSAFPYGGVLAGTLENASFWELKMGFSHWPHTLPMEPGGYVDFQTESSTRHCLVTYRPDKNHSTLRSVLMEMSYKLDDAGEPVCICHPVQTFLGGPTCKLLTKSAIFQNPENDGSILVCTGDEASNSALLWDAGSGSLLQELQAGQPVLDICPFEVNHSSYLATLTEKVVHIYRWE

Target ORF information:

RefSeq Version	XM_005620698.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ring finger and WD repeat domain 3 (RFWD3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005620698.2

ORF Insert Sequence:

ATGAACAGAG CGGCCAGGAA ACCCGCCCAA GTGATGGCTC ACGAAGCAAT GGAATATAAC 
CTTCATGGGC AGTTAGACCA TCATGTTGCA GAACAGCCTG TTCCTGCTGA AGTGGTCATC 
AGCCTAGAGG GACCACCCGT GTTCCAGGCT CTACCTGCAG AAGTGGTCAT CAGCCAAGAG 
GGACCACCCG TGCTCCAGGC TCTACCTGCA GAAGTGGTCA GCAGCCAAGA GGGACCACCC 
CTGCCCCAGC CTGCTCCACA CGTGTCTGTT GGCCTGACAG AGGAAGGGGA GCTGTTGGGA 
GAAGATCGTG TGGAGAGCAT TAATCCAGGA GCTTCGGAGG AGCATAGTCA GCGATCTCGT 
GTTAACCCCC CTGTCCAAGT GTCTTCTTTG GATTCGATGA ACAGCTTCAT CAGTGGGCTG 
CAGAGACTTC ATGGCATGCT GGAATTCCTG AGACCTCCAT CAGACCACAA CGTGGGACCA 
GTGAGATCCA GAAGGATGAG AGGATCTTCA CGCAGGTCAA GAACTACAGG GTCACAGAGG 
ACAGACAGTA CCAGGTCCAG AGCACCATTG GATGCCTACT TTCAAGTGAG CCGGACCCAG 
CCTCATTTGC CAACCACCTC CCGTGATTCA GAGACCAGAA ATCCTGTTTC TGAAGACTTG 
CAGGTCTCAA GTAGTTCCAA TTCTGACAGC GATAGCTCCA CAGAGTTTGA AGAAGTTGTT 
GCCCAGGCAG AGGAGACTAG AGCTGCTGTT TTAGAAGAGC AAGTAGAAGG TTCCTCAGCA 
CAGCAAGAAG TTACCTGTAT CAGTGGAGGA GAGACCTTCC CCAAACAGTC TCCCCAGAAG 
ACGGACCCCC TTCTACCTTC TGTTCCTATG GATGATGAAG AAGGGGACAC TTGCACAATC 
TGCTTGGAGC ACTGGACCAA TGCTGGGGAT CACCGCCTCT CAGCATTACG CTGTGGGCAC 
CTCTTTGGGT ATAAGTGCAT TTCTAAGTGG CTAAAGGGAC AGGCACGAAA ATGCCCCCAG 
TGCAACAAGA AAGCCAAGCA CAGTGACATC GTCGTTCTTT ACGCCCGAAC CCTAAGAGCT 
CTGGACACTA GTGAACACGA GCACATGAAA AGTTCCTTAC TAAAGGAGCA GATGCTAAGG 
AAGCAGGCGG AGTTAGAATC AGCACAGTGC CGGCTCCAGC TTCAAGTACT CACTGACGAA 
TGCAGCAAGC TTCATAGTCG TGTCCAGGAC TTGCAGAAAC TTATCGTGCA GCATCGGGAG 
CAGATTTCAC AGCACCCCAG GGGCTCCCAA GCATGTTTCC CAAACTGCCT GCCCTCCAGC 
CAGAGCCAGC ACAAGTACCA TTTCCAGAAG ACCTTCACAG TGTCTCAGAC AGGAAACTGC 
CGAATCATGA CATACTGTGA CACTCTGAGC TGCCTGGTGA TGTCACAGCC TTCCCCTGAG 
GCCTCCTTCC TGCCAGGCTT TGGCGTTAAG ATGTTGAGTA CTGCCAACAT GAAAAGCAGT 
CAGTACATTC CCATGCATGG CAAACAGATC CGAGGACTGG CTTTCAGTAG TCGGTCCAAA 
GGCTTACTAC TCTCGGCTTC CCTAGACAGC ACTGTGAAGC TAACCAGCCT AGAGACAAAT 
ACAGTGGTCC AGACATATAA TGCAGGACGT CCTGTCTGGA GCTGCTGCTG GTGTCTTGAT 
GAAAGTAATC ATATCTATGC CGGTCTGGTC AATGGTTCGA TTCTGCTGTA TGACCTTCGA 
AACACGAGCT CTCATATCCA GGAGTTAGCA CCCCAGAAAG CCAGATGCCC GCTGGTGTCC 
CTATCCTACA TACCCAGAGC TGCCTCATCT GCATTTCCAT ATGGTGGGGT GTTGGCTGGA 
ACTTTGGAGA ACGCTTCCTT CTGGGAGCTA AAAATGGGCT TTTCTCATTG GCCTCATACG 
CTGCCCATGG AGCCAGGGGG CTATGTAGAC TTTCAGACAG AGAGCAGCAC ACGGCACTGT 
CTTGTGACCT ACAGGCCCGA TAAAAATCAC AGCACTTTAC GAAGTGTGCT GATGGAGATG 
TCCTACAAGC TGGATGATGC TGGAGAGCCA GTCTGTATCT GCCATCCTGT ACAAACGTTC 
CTTGGGGGAC CCACTTGCAA ACTACTGACC AAAAGTGCCA TTTTCCAAAA CCCAGAGAAT 
GATGGCAGCA TCCTGGTGTG TACTGGGGAC GAAGCATCAA ATTCTGCCTT GCTGTGGGAT 
GCTGGCAGTG GCTCGTTGCT GCAGGAACTG CAGGCTGGCC AGCCTGTCTT GGACATCTGC 
CCATTTGAGG TGAACCACAG TAGCTACTTG GCTACCTTAA CAGAGAAGGT GGTCCACATC 
TATAGGTGGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RFWD3 ( XM_005620698.3 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005620755.1 Canis lupus familiaris ring finger and WD repeat domain 3 (RFWD3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe07103
Clone ID Related Accession (Same CDS sequence)	XM_005620698.2 , XM_005620698.3
Accession Version	XM_005620698.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase RFWD3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005620698.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAGAG CGGCCAGGAA ACCCGCCCAA GTGATGGCTC ACGAAGCAAT GGAATATAAC 
CTTCATGGGC AGTTAGACCA TCATGTTGCA GAACAGCCTG TTCCTGCTGA AGTGGTCATC 
AGCCTAGAGG GACCACCCGT GTTCCAGGCT CTACCTGCAG AAGTGGTCAT CAGCCAAGAG 
GGACCACCCG TGCTCCAGGC TCTACCTGCA GAAGTGGTCA GCAGCCAAGA GGGACCACCC 
CTGCCCCAGC CTGCTCCACA CGTGTCTGTT GGCCTGACAG AGGAAGGGGA GCTGTTGGGA 
GAAGATCGTG TGGAGAGCAT TAATCCAGGA GCTTCGGAGG AGCATAGTCA GCGATCTCGT 
GTTAACCCCC CTGTCCAAGT GTCTTCTTTG GATTCGATGA ACAGCTTCAT CAGTGGGCTG 
CAGAGACTTC ATGGCATGCT GGAATTCCTG AGACCTCCAT CAGACCACAA CGTGGGACCA 
GTGAGATCCA GAAGGATGAG AGGATCTTCA CGCAGGTCAA GAACTACAGG GTCACAGAGG 
ACAGACAGTA CCAGGTCCAG AGCACCATTG GATGCCTACT TTCAAGTGAG CCGGACCCAG 
CCTCATTTGC CAACCACCTC CCGTGATTCA GAGACCAGAA ATCCTGTTTC TGAAGACTTG 
CAGGTCTCAA GTAGTTCCAA TTCTGACAGC GATAGCTCCA CAGAGTTTGA AGAAGTTGTT 
GCCCAGGCAG AGGAGACTAG AGCTGCTGTT TTAGAAGAGC AAGTAGAAGG TTCCTCAGCA 
CAGCAAGAAG TTACCTGTAT CAGTGGAGGA GAGACCTTCC CCAAACAGTC TCCCCAGAAG 
ACGGACCCCC TTCTACCTTC TGTTCCTATG GATGATGAAG AAGGGGACAC TTGCACAATC 
TGCTTGGAGC ACTGGACCAA TGCTGGGGAT CACCGCCTCT CAGCATTACG CTGTGGGCAC 
CTCTTTGGGT ATAAGTGCAT TTCTAAGTGG CTAAAGGGAC AGGCACGAAA ATGCCCCCAG 
TGCAACAAGA AAGCCAAGCA CAGTGACATC GTCGTTCTTT ACGCCCGAAC CCTAAGAGCT 
CTGGACACTA GTGAACACGA GCACATGAAA AGTTCCTTAC TAAAGGAGCA GATGCTAAGG 
AAGCAGGCGG AGTTAGAATC AGCACAGTGC CGGCTCCAGC TTCAAGTACT CACTGACGAA 
TGCAGCAAGC TTCATAGTCG TGTCCAGGAC TTGCAGAAAC TTATCGTGCA GCATCGGGAG 
CAGATTTCAC AGCACCCCAG GGGCTCCCAA GCATGTTTCC CAAACTGCCT GCCCTCCAGC 
CAGAGCCAGC ACAAGTACCA TTTCCAGAAG ACCTTCACAG TGTCTCAGAC AGGAAACTGC 
CGAATCATGA CATACTGTGA CACTCTGAGC TGCCTGGTGA TGTCACAGCC TTCCCCTGAG 
GCCTCCTTCC TGCCAGGCTT TGGCGTTAAG ATGTTGAGTA CTGCCAACAT GAAAAGCAGT 
CAGTACATTC CCATGCATGG CAAACAGATC CGAGGACTGG CTTTCAGTAG TCGGTCCAAA 
GGCTTACTAC TCTCGGCTTC CCTAGACAGC ACTGTGAAGC TAACCAGCCT AGAGACAAAT 
ACAGTGGTCC AGACATATAA TGCAGGACGT CCTGTCTGGA GCTGCTGCTG GTGTCTTGAT 
GAAAGTAATC ATATCTATGC CGGTCTGGTC AATGGTTCGA TTCTGCTGTA TGACCTTCGA 
AACACGAGCT CTCATATCCA GGAGTTAGCA CCCCAGAAAG CCAGATGCCC GCTGGTGTCC 
CTATCCTACA TACCCAGAGC TGCCTCATCT GCATTTCCAT ATGGTGGGGT GTTGGCTGGA 
ACTTTGGAGA ACGCTTCCTT CTGGGAGCTA AAAATGGGCT TTTCTCATTG GCCTCATACG 
CTGCCCATGG AGCCAGGGGG CTATGTAGAC TTTCAGACAG AGAGCAGCAC ACGGCACTGT 
CTTGTGACCT ACAGGCCCGA TAAAAATCAC AGCACTTTAC GAAGTGTGCT GATGGAGATG 
TCCTACAAGC TGGATGATGC TGGAGAGCCA GTCTGTATCT GCCATCCTGT ACAAACGTTC 
CTTGGGGGAC CCACTTGCAA ACTACTGACC AAAAGTGCCA TTTTCCAAAA CCCAGAGAAT 
GATGGCAGCA TCCTGGTGTG TACTGGGGAC GAAGCATCAA ATTCTGCCTT GCTGTGGGAT 
GCTGGCAGTG GCTCGTTGCT GCAGGAACTG CAGGCTGGCC AGCCTGTCTT GGACATCTGC 
CCATTTGAGG TGAACCACAG TAGCTACTTG GCTACCTTAA CAGAGAAGGT GGTCCACATC 
TATAGGTGGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005620755.1
CDS	205..2559
Translation	MNRAARKPAQVMAHEAMEYNLHGQLDHHVAEQPVPAEVVISLEGPPVFQALPAEVVISQEGPPVLQALPAEVVSSQEGPPLPQPAPHVSVGLTEEGELLGEDRVESINPGASEEHSQRSRVNPPVQVSSLDSMNSFISGLQRLHGMLEFLRPPSDHNVGPVRSRRMRGSSRRSRTTGSQRTDSTRSRAPLDAYFQVSRTQPHLPTTSRDSETRNPVSEDLQVSSSSNSDSDSSTEFEEVVAQAEETRAAVLEEQVEGSSAQQEVTCISGGETFPKQSPQKTDPLLPSVPMDDEEGDTCTICLEHWTNAGDHRLSALRCGHLFGYKCISKWLKGQARKCPQCNKKAKHSDIVVLYARTLRALDTSEHEHMKSSLLKEQMLRKQAELESAQCRLQLQVLTDECSKLHSRVQDLQKLIVQHREQISQHPRGSQACFPNCLPSSQSQHKYHFQKTFTVSQTGNCRIMTYCDTLSCLVMSQPSPEASFLPGFGVKMLSTANMKSSQYIPMHGKQIRGLAFSSRSKGLLLSASLDSTVKLTSLETNTVVQTYNAGRPVWSCCWCLDESNHIYAGLVNGSILLYDLRNTSSHIQELAPQKARCPLVSLSYIPRAASSAFPYGGVLAGTLENASFWELKMGFSHWPHTLPMEPGGYVDFQTESSTRHCLVTYRPDKNHSTLRSVLMEMSYKLDDAGEPVCICHPVQTFLGGPTCKLLTKSAIFQNPENDGSILVCTGDEASNSALLWDAGSGSLLQELQAGQPVLDICPFEVNHSSYLATLTEKVVHIYRWE

Target ORF information:

RefSeq Version	XM_005620698.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ring finger and WD repeat domain 3 (RFWD3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005620698.3

ORF Insert Sequence:

ATGAACAGAG CGGCCAGGAA ACCCGCCCAA GTGATGGCTC ACGAAGCAAT GGAATATAAC 
CTTCATGGGC AGTTAGACCA TCATGTTGCA GAACAGCCTG TTCCTGCTGA AGTGGTCATC 
AGCCTAGAGG GACCACCCGT GTTCCAGGCT CTACCTGCAG AAGTGGTCAT CAGCCAAGAG 
GGACCACCCG TGCTCCAGGC TCTACCTGCA GAAGTGGTCA GCAGCCAAGA GGGACCACCC 
CTGCCCCAGC CTGCTCCACA CGTGTCTGTT GGCCTGACAG AGGAAGGGGA GCTGTTGGGA 
GAAGATCGTG TGGAGAGCAT TAATCCAGGA GCTTCGGAGG AGCATAGTCA GCGATCTCGT 
GTTAACCCCC CTGTCCAAGT GTCTTCTTTG GATTCGATGA ACAGCTTCAT CAGTGGGCTG 
CAGAGACTTC ATGGCATGCT GGAATTCCTG AGACCTCCAT CAGACCACAA CGTGGGACCA 
GTGAGATCCA GAAGGATGAG AGGATCTTCA CGCAGGTCAA GAACTACAGG GTCACAGAGG 
ACAGACAGTA CCAGGTCCAG AGCACCATTG GATGCCTACT TTCAAGTGAG CCGGACCCAG 
CCTCATTTGC CAACCACCTC CCGTGATTCA GAGACCAGAA ATCCTGTTTC TGAAGACTTG 
CAGGTCTCAA GTAGTTCCAA TTCTGACAGC GATAGCTCCA CAGAGTTTGA AGAAGTTGTT 
GCCCAGGCAG AGGAGACTAG AGCTGCTGTT TTAGAAGAGC AAGTAGAAGG TTCCTCAGCA 
CAGCAAGAAG TTACCTGTAT CAGTGGAGGA GAGACCTTCC CCAAACAGTC TCCCCAGAAG 
ACGGACCCCC TTCTACCTTC TGTTCCTATG GATGATGAAG AAGGGGACAC TTGCACAATC 
TGCTTGGAGC ACTGGACCAA TGCTGGGGAT CACCGCCTCT CAGCATTACG CTGTGGGCAC 
CTCTTTGGGT ATAAGTGCAT TTCTAAGTGG CTAAAGGGAC AGGCACGAAA ATGCCCCCAG 
TGCAACAAGA AAGCCAAGCA CAGTGACATC GTCGTTCTTT ACGCCCGAAC CCTAAGAGCT 
CTGGACACTA GTGAACACGA GCACATGAAA AGTTCCTTAC TAAAGGAGCA GATGCTAAGG 
AAGCAGGCGG AGTTAGAATC AGCACAGTGC CGGCTCCAGC TTCAAGTACT CACTGACGAA 
TGCAGCAAGC TTCATAGTCG TGTCCAGGAC TTGCAGAAAC TTATCGTGCA GCATCGGGAG 
CAGATTTCAC AGCACCCCAG GGGCTCCCAA GCATGTTTCC CAAACTGCCT GCCCTCCAGC 
CAGAGCCAGC ACAAGTACCA TTTCCAGAAG ACCTTCACAG TGTCTCAGAC AGGAAACTGC 
CGAATCATGA CATACTGTGA CACTCTGAGC TGCCTGGTGA TGTCACAGCC TTCCCCTGAG 
GCCTCCTTCC TGCCAGGCTT TGGCGTTAAG ATGTTGAGTA CTGCCAACAT GAAAAGCAGT 
CAGTACATTC CCATGCATGG CAAACAGATC CGAGGACTGG CTTTCAGTAG TCGGTCCAAA 
GGCTTACTAC TCTCGGCTTC CCTAGACAGC ACTGTGAAGC TAACCAGCCT AGAGACAAAT 
ACAGTGGTCC AGACATATAA TGCAGGACGT CCTGTCTGGA GCTGCTGCTG GTGTCTTGAT 
GAAAGTAATC ATATCTATGC CGGTCTGGTC AATGGTTCGA TTCTGCTGTA TGACCTTCGA 
AACACGAGCT CTCATATCCA GGAGTTAGCA CCCCAGAAAG CCAGATGCCC GCTGGTGTCC 
CTATCCTACA TACCCAGAGC TGCCTCATCT GCATTTCCAT ATGGTGGGGT GTTGGCTGGA 
ACTTTGGAGA ACGCTTCCTT CTGGGAGCTA AAAATGGGCT TTTCTCATTG GCCTCATACG 
CTGCCCATGG AGCCAGGGGG CTATGTAGAC TTTCAGACAG AGAGCAGCAC ACGGCACTGT 
CTTGTGACCT ACAGGCCCGA TAAAAATCAC AGCACTTTAC GAAGTGTGCT GATGGAGATG 
TCCTACAAGC TGGATGATGC TGGAGAGCCA GTCTGTATCT GCCATCCTGT ACAAACGTTC 
CTTGGGGGAC CCACTTGCAA ACTACTGACC AAAAGTGCCA TTTTCCAAAA CCCAGAGAAT 
GATGGCAGCA TCCTGGTGTG TACTGGGGAC GAAGCATCAA ATTCTGCCTT GCTGTGGGAT 
GCTGGCAGTG GCTCGTTGCT GCAGGAACTG CAGGCTGGCC AGCCTGTCTT GGACATCTGC 
CCATTTGAGG TGAACCACAG TAGCTACTTG GCTACCTTAA CAGAGAAGGT GGTCCACATC 
TATAGGTGGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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RFWD3 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

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