NECTIN4 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	NECTIN4
Entrez Gene ID	609931
Full Name	nectin cell adhesion molecule 4
Synonyms	nectin-4
General protein information	Preferred Names nectin cell adhesion molecule 4 Names nectin-4 nectin 4
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001313853.1	NP_001300782.1	nectin-4 precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PVRL4	XP_005641000.1
Mus musculus (house mouse)	Pvrl4	NP_082169.2
	PVRL4	XP_001117709.1
Rattus norvegicus (Norway rat)	Pvrl4	NP_001102546.1
Bos taurus (cattle)	PVRL4	NP_001019665.1
Danio rerio (zebrafish)	LOC560512	XP_005172277.1
Homo sapiens (human)	PVRL4	NP_112178.2
Pan troglodytes (chimpanzee)	PVRL4	XP_003308602.1
Danio rerio (zebrafish)	pvrl4	XP_001334985.4

Gene Ontology
Bibliography

Related articles in PubMed

Sequential conformational changes in the morbillivirus attachment protein initiate the membrane fusion process.
Ader-Ebert N, Khosravi M, Herren M, Avila M, Alves L, Bringolf F, ?rvell C, Langedijk JP, Zurbriggen A, Plemper RK, Plattet P
PLoS pathogens11(5)e1004880(2015 May)

The V domain of dog PVRL4 (nectin-4) mediates canine distemper virus entry and virus cell-to-cell spread.
Delpeut S, Noyce RS, Richardson CD
Virology454-455109-17(2014 Apr)

Use of SLAM and PVRL4 and identification of pro-HB-EGF as cell entry receptors for wild type phocine distemper virus.
Melia MM, Earle JP, Abdullah H, Reaney K, Tangy F, Cosby SL
PloS one9(8)e106281(2014)

Dog nectin-4 is an epithelial cell receptor for canine distemper virus that facilitates virus entry and syncytia formation.
Noyce RS, Delpeut S, Richardson CD
Virology436(1)210-20(2013 Feb)

Canine distemper virus with the intact C protein has the potential to replicate in human epithelial cells by using human nectin4 as a receptor.
Otsuki N, Sekizuka T, Seki F, Sakai K, Kubota T, Nakatsu Y, Chen S, Fukuhara H, Maenaka K, Yamaguchi R, Kuroda M, Takeda M
Virology435(2)485-92(2013 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that conformational changes in Morbillivirus attachment glycoprotein H as it interacts with nectin-4 or SLAM (signaling lymphocytic activation molecule family member 1) are required for membrane fusion/virus internalization.
Title: Sequential conformational changes in the morbillivirus attachment protein initiate the membrane fusion process.

In this study, authors demonstrate that the V domain of PVRL4 is critical for canine distemper virus entry and virus cell-to-cell spread.
Title: The V domain of dog PVRL4 (nectin-4) mediates canine distemper virus entry and virus cell-to-cell spread.

Use of SLAM and PVRL4 and identification of pro-HB-EGF as cell entry receptors for wild type phocine distemper virus.
Title: Use of SLAM and PVRL4 and identification of pro-HB-EGF as cell entry receptors for wild type phocine distemper virus.

data confirm that dog nectin-4 can be used by Canine distemper virus to gain entry into epithelial cells, and facilitate virus spread
Title: Dog nectin-4 is an epithelial cell receptor for canine distemper virus that facilitates virus entry and syncytia formation.

In dogs with distemper, CDV antigen was preferentially detected in nectin4-positive neurons and epithelial cells, suggesting that nectin4 is an epithelial cell receptor for CDV and also involved in its neurovirulence.
Title: Nectin4 is an epithelial cell receptor for canine distemper virus and involved in neurovirulence.

The following NECTIN4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NECTIN4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe140204	NM_001313853.1 Latest version!	Canis lupus familiaris nectin cell adhesion molecule 4 (NECTIN4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NECTIN4 ( NM_001313853.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001300782.1 Canis lupus familiaris nectin cell adhesion molecule 4 (NECTIN4), mRNA.

Price & Availability↑

CloneID	OCe140204
Clone ID Related Accession (Same CDS sequence)	NM_001313853.1
Accession Version	NM_001313853.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Canis lupus familiaris(dog)
Product	nectin-4 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB755429.1. ##Evidence-Data-START## Transcript exon combination :: AB755429.1, JX629803.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA103936001, SAMEA103936002 [ECO:0000348] ##Evidence-Data-END##

ATGCCTCTGT CCCTGGGAGC CGAGATGTGG GGGCCCGAGG TCCTGCTGCT GCTGCTGCTC 
CTGGCGTCAT TCACAGGCCG GTGCCGGCCG GGTGAGCTGG AGACCTCGGA CCTGGTGACC 
GTGGTGCTGG GCCAGGACGC CAAGCTGCCC TGCTTCTACC GAGGAGACCC CGGCGAGCAG 
GTGGGGCAGG TGGCGTGGGC TCGCGTGGAC GCGGGCGAGG GCGCCCGGGA GCTGGCGCTG 
CTGCACTCCA AGTACGGGCT GCACGTGAGC GCGGCCTACG AGGGCCGCGT GGAGCAGCCG 
CCGCCACCAC GGAGCCCCCT GGACGGCGCC GTGCTCCTGC GCAACGCGGT GCAGGCCGAC 
GAGGGCGAGT ACGAGTGCCG CGTCAGCACC TTCCCCGCCG GCAGCTTCCA GGCGAGGCTG 
CGGCTCCGCG TGCTGGTGCC CCCCCTGCCC TCCCTGAACC CCGGGCCAGC GCTCGAAGAG 
GGCCAGGGCC TGACGCTGGC AGCCTCCTGC ACAGCAGAGG GCAGCCCAGC CCCCAGCGTG 
ACCTGGGACA CGGAGGTCAA GGGCACGGCG TCCAGCCGTT CCTTCAAGCA CTCCCGCTCA 
GCGGCCGTCA CTTCGGAGTT CCACCTGGTG CCCAGCCGCA GCATGAACGG GCAGCCGCTC 
ACATGCGTGG TGTCCCACCC TGGCCTGCTC CAGGACCAGA GGATCACCCA CGTCCTCCAG 
GTGGCCTTCC TCGCTGAGGC CTCCGTGAGG GGCCTTGAAG ACCAAAAGCT GTGGCAGGTT 
GGCAGAGAAG GCGCTACACT CAAGTGCCTG AGCGAGGGGC ACCCCCCTCC CTCGTACAAC 
TGGACACGGT TGGACGGGCC CCTGCCCAGC GGCGTCCGTG TGCAGGGGGA CACGCTGGGC 
TTTCCTCCGC TGACCGCCGA GCACAGCGGC ACCTACGTCT GCCACGTCAG CAATGAGCTG 
TCCTCCAGGG ACTCCCAGGT CACGGTGGAC GTCCTAGACC CAGAGGAGGC CCCGGGGAAG 
CAGGTGGACC TGGTGTCGGC GTCCGTGGTG GTGGTGGGGG TGATCGCCGC GCTCCTGTTC 
TGCCTCCTGG TGGTGGTGGT GGTGCTCATG TCCCGGTACC ATCGGCGCAA GGCTCAGCAG 
ATGACCCAGA AATACGAGGA GGAGCTGACC CTGACCAGGG AGAACTCCAT CCGGAGGCTG 
CACTCCCACC ACTCGGACCC CAGGAGCCAG CCGGAGGAGA GTGTAGGGCT GAGAGCCGAG 
GGCCACCCCG ATAGTCTCAA GGACAACAGT AGCTGCTCTG TGATGAGTGA AGAGCCCGAG 
GGCCGCAGCT ACTCCACGCT GACCACCGTG CGGGAGATTG AGACACAGAC CGAACTGCTG 
TCCCCAGGCT CCGGGCGGGC AGAGGACGAG GAAGACCGGG ATGAAGGCAT CAAACAGGCC 
ATGAACCATT TTGTTCAAGA GAACGGAACC CTGCGGGCCA AACCCACGGG CAACGGCATC 
TACATCAACG GGCGGGGGCA TCTGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300782.1
CDS	1..1530
Translation	MPLSLGAEMWGPEVLLLLLLLASFTGRCRPGELETSDLVTVVLGQDAKLPCFYRGDPGEQVGQVAWARVDAGEGARELALLHSKYGLHVSAAYEGRVEQPPPPRSPLDGAVLLRNAVQADEGEYECRVSTFPAGSFQARLRLRVLVPPLPSLNPGPALEEGQGLTLAASCTAEGSPAPSVTWDTEVKGTASSRSFKHSRSAAVTSEFHLVPSRSMNGQPLTCVVSHPGLLQDQRITHVLQVAFLAEASVRGLEDQKLWQVGREGATLKCLSEGHPPPSYNWTRLDGPLPSGVRVQGDTLGFPPLTAEHSGTYVCHVSNELSSRDSQVTVDVLDPEEAPGKQVDLVSASVVVVGVIAALLFCLLVVVVVLMSRYHRRKAQQMTQKYEEELTLTRENSIRRLHSHHSDPRSQPEESVGLRAEGHPDSLKDNSSCSVMSEEPEGRSYSTLTTVREIETQTELLSPGSGRAEDEEDRDEGIKQAMNHFVQENGTLRAKPTGNGIYINGRGHLV

Target ORF information:

RefSeq Version	NM_001313853.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris nectin cell adhesion molecule 4 (NECTIN4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313853.1

ORF Insert Sequence:

ATGCCTCTGT CCCTGGGAGC CGAGATGTGG GGGCCCGAGG TCCTGCTGCT GCTGCTGCTC 
CTGGCGTCAT TCACAGGCCG GTGCCGGCCG GGTGAGCTGG AGACCTCGGA CCTGGTGACC 
GTGGTGCTGG GCCAGGACGC CAAGCTGCCC TGCTTCTACC GAGGAGACCC CGGCGAGCAG 
GTGGGGCAGG TGGCGTGGGC TCGCGTGGAC GCGGGCGAGG GCGCCCGGGA GCTGGCGCTG 
CTGCACTCCA AGTACGGGCT GCACGTGAGC GCGGCCTACG AGGGCCGCGT GGAGCAGCCG 
CCGCCACCAC GGAGCCCCCT GGACGGCGCC GTGCTCCTGC GCAACGCGGT GCAGGCCGAC 
GAGGGCGAGT ACGAGTGCCG CGTCAGCACC TTCCCCGCCG GCAGCTTCCA GGCGAGGCTG 
CGGCTCCGCG TGCTGGTGCC CCCCCTGCCC TCCCTGAACC CCGGGCCAGC GCTCGAAGAG 
GGCCAGGGCC TGACGCTGGC AGCCTCCTGC ACAGCAGAGG GCAGCCCAGC CCCCAGCGTG 
ACCTGGGACA CGGAGGTCAA GGGCACGGCG TCCAGCCGTT CCTTCAAGCA CTCCCGCTCA 
GCGGCCGTCA CTTCGGAGTT CCACCTGGTG CCCAGCCGCA GCATGAACGG GCAGCCGCTC 
ACATGCGTGG TGTCCCACCC TGGCCTGCTC CAGGACCAGA GGATCACCCA CGTCCTCCAG 
GTGGCCTTCC TCGCTGAGGC CTCCGTGAGG GGCCTTGAAG ACCAAAAGCT GTGGCAGGTT 
GGCAGAGAAG GCGCTACACT CAAGTGCCTG AGCGAGGGGC ACCCCCCTCC CTCGTACAAC 
TGGACACGGT TGGACGGGCC CCTGCCCAGC GGCGTCCGTG TGCAGGGGGA CACGCTGGGC 
TTTCCTCCGC TGACCGCCGA GCACAGCGGC ACCTACGTCT GCCACGTCAG CAATGAGCTG 
TCCTCCAGGG ACTCCCAGGT CACGGTGGAC GTCCTAGACC CAGAGGAGGC CCCGGGGAAG 
CAGGTGGACC TGGTGTCGGC GTCCGTGGTG GTGGTGGGGG TGATCGCCGC GCTCCTGTTC 
TGCCTCCTGG TGGTGGTGGT GGTGCTCATG TCCCGGTACC ATCGGCGCAA GGCTCAGCAG 
ATGACCCAGA AATACGAGGA GGAGCTGACC CTGACCAGGG AGAACTCCAT CCGGAGGCTG 
CACTCCCACC ACTCGGACCC CAGGAGCCAG CCGGAGGAGA GTGTAGGGCT GAGAGCCGAG 
GGCCACCCCG ATAGTCTCAA GGACAACAGT AGCTGCTCTG TGATGAGTGA AGAGCCCGAG 
GGCCGCAGCT ACTCCACGCT GACCACCGTG CGGGAGATTG AGACACAGAC CGAACTGCTG 
TCCCCAGGCT CCGGGCGGGC AGAGGACGAG GAAGACCGGG ATGAAGGCAT CAAACAGGCC 
ATGAACCATT TTGTTCAAGA GAACGGAACC CTGCGGGCCA AACCCACGGG CAACGGCATC 
TACATCAACG GGCGGGGGCA TCTGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequential conformational changes in the morbillivirus attachment protein initiate the membrane fusion process.
PLoS pathogens11(5)e1004880(2015 May)
Ader-Ebert N,Khosravi M,Herren M,Avila M,Alves L,Bringolf F,?rvell C,Langedijk JP,Zurbriggen A,Plemper RK,Plattet P

The V domain of dog PVRL4 (nectin-4) mediates canine distemper virus entry and virus cell-to-cell spread.
Virology454-455109-17(2014 Apr)
Delpeut S,Noyce RS,Richardson CD

Use of SLAM and PVRL4 and identification of pro-HB-EGF as cell entry receptors for wild type phocine distemper virus.
PloS one9(8)e106281(2014)
Melia MM,Earle JP,Abdullah H,Reaney K,Tangy F,Cosby SL

Dog nectin-4 is an epithelial cell receptor for canine distemper virus that facilitates virus entry and syncytia formation.
Virology436(1)210-20(2013 Feb)
Noyce RS,Delpeut S,Richardson CD

Canine distemper virus with the intact C protein has the potential to replicate in human epithelial cells by using human nectin4 as a receptor.
Virology435(2)485-92(2013 Jan)
Otsuki N,Sekizuka T,Seki F,Sakai K,Kubota T,Nakatsu Y,Chen S,Fukuhara H,Maenaka K,Yamaguchi R,Kuroda M,Takeda M

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NECTIN4 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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