RAPGEF6 gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_014117698.1	XP_013973173.1	rap guanine nucleotide exchange factor 6 isoform X3
XM_014117703.1	XP_013973178.1	rap guanine nucleotide exchange factor 6 isoform X10
XM_014117697.2	XP_013973172.1	rap guanine nucleotide exchange factor 6 isoform X1
XM_014117702.2	XP_013973177.1	rap guanine nucleotide exchange factor 6 isoform X9
XM_014117699.2	XP_013973174.1	rap guanine nucleotide exchange factor 6 isoform X6
XM_014117701.2	XP_013973176.1	rap guanine nucleotide exchange factor 6 isoform X7
XM_014117703.2	XP_013973178.1	rap guanine nucleotide exchange factor 6 isoform X10
XM_005626500.3	XP_005626557.1	rap guanine nucleotide exchange factor 6 isoform X11
XM_846793.4	XP_851886.2	rap guanine nucleotide exchange factor 6 isoform X4
XM_003431622.4	XP_003431670.1	rap guanine nucleotide exchange factor 6 isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe39111	XM_014117702.2 Latest version!	Canis lupus familiaris Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCe39112	XM_014117703.2 Latest version!	Canis lupus familiaris Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCe39106	XM_014117697.2 Latest version!	Canis lupus familiaris Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe39107	XM_014117698.1 Latest version!	Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 6 (RAPGEF6), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe39108	XM_014117699.2 Latest version!	Canis lupus familiaris Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe39110	XM_014117701.2 Latest version!	Canis lupus familiaris Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe14233	XM_003431622.4 Latest version!	Canis lupus familiaris Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe14234	XM_846793.4 Latest version!	Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 6 (RAPGEF6), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe14241	XM_005626500.3 Latest version!	Canis lupus familiaris Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
OCe39112	XM_014117703.1	Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 6 (RAPGEF6), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
Hide Old Accession Versions >>

RAPGEF6 ( XM_014117702.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013973177.1 Canis lupus familiaris Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OCe39111
Clone ID Related Accession (Same CDS sequence)	XM_014117702.2
Accession Version	XM_014117702.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	rap guanine nucleotide exchange factor 6 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014117702.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTTC TTGTTCGGTG TTATGCCAAT TGTCTTCAGC CATGGGCCTC CAAACTTCCA 
GCCGATCTTA CCAAGATGCA CATCACAGAC AACCCTCATC CACAGGTGAC TCATGTGTCT 
TCTAGTCAGT CTGGTTGTAG CATCGCCAGT GACTCTGGGA GCAGCAGTTT ATCTGATATC 
TATCAGGCTA CGGAAAGTGA GGTAGGAGAT GTAGATTTAA CACGTCTTCC AGAAGGACCT 
GTTGATTCTG AAGATGAAGA AGAGGAAGAT GAAGAAATTG ATCGAACGGA TCCACTACAG 
GGGCGAGATC TTGTTCGAGA ATGTCTTGAG AAAGAACCTG CAGACAAAAC TGATGATGAC 
ATTGAACAAT TACTGGAGTT TATGCACCAG CTCCCAGCTT TTGCAAACAT GACCATGTCT 
GTAAGAAGAG AACTCTGCTC AGTGATGATT TTTGAAGTGG TGGAGCAGGC TGGAGCTATT 
ATTCTTGAAG ATGGGCAAGA GCTTGACTCA TGGTATGTTA TTTTAAACGG CACTGTAGAA 
ATCACTCATC CAGATGGAAA AGTTGAAAAT CTCTTTATGG GAAATAGTTT TGGAATTACT 
CCCACTCTGG ACAAACAGTA CATGCATGGA GTTGTTAGGA CGAAAGTAGA TGATTGTCAG 
TTTGTCTGCA TAGCGCAGCA GGATTATTGG AGAATTTTAA ATCATGTGGA GAAAAACACC 
CATAAAGTTG AGGAAGAGGG AGAAATTGTC ATGGTACATG AGCATCGTGA ACTAGATCGG 
AGTGGAACCA GGAAAGGACA CATTGTAATC AAGGCAACAC CTGAGCGTCT CATCATGCAT 
TTAATAGAAG AGCATTCCAT TGTGGACCCG ACTTATATAG AAGATTTCCT GCTAACTTAC 
AGGACATTTC TTGAAAGTCC TTTGGATGTT GGGATCAAAC TATTGGAATG GTTTAAAATT 
GACAGCTTAA GGGATAAGGT AACAAGGATT GTGTTATTAT GGGTAAATAA TCATTTTAAT 
GATTTTGAAG GTGATCCTGC TATGACTCGA TTTCTGGAGG AATTTGAAAA AAATCTGGAA 
GATACGAAAA TGAATGGTCA TCTCCGGTTA TTGAATATTG CCTGTGCTGC AAAGGCCAAG 
TGGAGACAGG TGGTGCTGCA AAAGGCTTCC CGGGAGTCCC CTCTGCATTT CAGCCTTAAT 
GGAGGGAGTG AGAAGGGCTT TGGTATTTTT GTTGAAGGAG TAGAACCTAG TAGCAAAGCT 
GCTGATGCTG GACTGAAACG TGGTGATCAG ATAATGGAAG TAAATGGACA AAACTTTGAA 
AATATTACAT TTGTGAAAGC CCTTGAAATT TTAAGGAATA ATACTCATCT TGCACTTACT 
GTGAAGACCA ACATTTTTGT GTTCAAAGAA TTACTTAGTA GGACTGAACA AGAAAAATCT 
GGTGTTCCTC ATATTCCTAA AATTGCTGAA AAAAAAAGTA ATCGCCATTC AATCCAGGAT 
GTACCGGGAG ATATTGAACA CACATCACAG GAGAAAGGAA ATAAGAAAAT AAAAGCAAAC 
ACTGTTTCAG GTGGAAGAAA CAAAATTAGG AAAATTTTGG ATAAAACACG GTTTAGTATC 
TTACCTCCAA AACTGTTTAG GAGACTGTTT TGTGATGGAG GCCTGAGCCA GTCACAAGAT 
GACAGCATTG TGGGAACAAG ACACTGCAGG CACAGTCTGG CTATCATGCC TATTCCTGGA 
ACACTCTCAT CCAGCAGCCC TGATCTCTTG CAGCCTACCA CCAGCATGTT GGATTTTTCT 
AATCCTTCAG ATATTCCTGA TCAAGTTATA AGAGTTTTCA AAGCAGATCA ACAAAGTTGC 
TACATTATCA TCAGTAAAGA CACTACAGCT AAAGAAGTTG TCTGTCATGC TGTTCATGAA 
TTTGGTTTGA CTGGTGCATC CGACACGTAT TCTCTCTGTG AAGTTTCTGT TACTCCTGAG 
GGTGTCATAA AACAAAGAAG ACTTCCAGAC CAATTCTCCA AATTAGCTGA TAGAATTCAG 
CTCAATGGAA GATATTATTT AAAAAATAAC ATGGAAACAG AAACCCTATG TTCAGATGAA 
GATGCTCAAG AACTAGTTAA GGAAAGCCAA CTATCCATGC TGCAGCTCAG TACCATTGAG 
GTGGCCACTC AGCTGTCAAT GAGGGACTTT GATTTGTTTC GTAATATTGA GCCTACTGAA 
TACATTGATG ACCTTTTTAA GTTAGATTCC AAAACAGGAA ATACTCACTT GAAAGAGTTT 
GAGGACATTG TTAACCAGGA GACATTCTGG GTTGCCTCAG AGATTTTAAC GGAATCAAAT 
CAGTTGAAAC GAATGAAGAT TATTAAGCAT TTTATTAAAA TTGCACTTCA TTGTCGAGAA 
TGTAAGAACT TCAATTCCAT GTTTGCAATA ATAAGTGGCT TGAACCTGGC ATCTGTAGCA 
CGACTCAGAG GTACTTGGGA AAAATTACCA AGCAAGTATG AGAAACACCT TCAAGATCTA 
CAGGACCTTT TTGATCCATC TAGAAACATG GCAAAATATA GAAATATTCT TAGTAGTCAA 
AGCATGCAGC CTCCAATTAT TCCACTCTTC CCTGTTGTCA AGAAAGATAT GACATTTCTA 
CATGAAGGAA ATGACTCCAA AGTAGATGGT TTAGTGAATT TTGAGAAGCT AAGAATGATT 
GCCAAGGAGA TCCGTCAGGT TGTTCGAATG ACTTCTGCTA ACATGGACCC AGCTATGATG 
TTTCGACAGA GGAAGAAGAG GTGGCGGAGT CTGGGGTCAC TGAGTCAAGG CAGCACAAAC 
TCAAACATGC TGGATGTTCA AGGAGGTGCA CACAAGAAAA GGGCACGTCG CAGCTCTCTA 
CTTAATGCCA AGAAGTTATA CGAGGATGCC CAGATGGCAA GGAAAGTGAA GCAGTATCTT 
TCCAGTCTGG ATGTCGACAC AGATGAGGAA AAGTTTCAGA TGATGTCTTT GCAGTGGGAG 
CCTGCCTATG GTACCTTGAC CAAGAATTTA AGTGAGAAAA GATCAGCCAA ATCATCAGAA 
ATGTCTCCAG TGCCTATGAG ATCGGCAGGC CAAACAGCTA AAGCACATTT GCATCAGCCC 
CACCGAGTAA GCCAGGTGCT TCAAGTGCCA GCTGTTAATT TGCACCCCAT CAGGAAGAAG 
GGACAAGCCA AAGACCCTGC CACGTTGAGT ACAAATTTAC CTCAGAAAGT TTTAGGAACA 
ACTGAAGAAG TAAGTGGTAA GAAACATCCA GAAGACACTA TCTCTGTGGC ATCATCCTTA 
CATTCTAGCC CTCCTGCATC TCCTCAAGGT TCCCCTCGCA AAGGTTATAC ACTTACACCA 
TCAGCTAAAT CTGACAACTT GTCTGACTCC AGCCATAGTG AAATTTCTTC ACGATCCAGC 
ATTGTGAGCA ATTGTTCTGT TGACTCCATG TCTACAGCTC TACAGGATGA ACGGTGTTCT 
TCACAGGCTG TGGCGGTCCC TGAATCCACT GGGCCTTTGG AAAAGACAGA ACACTCCTCA 
GGGATAGGAG ACCATAGTCA GCTTGGCCCC GGGTGGACGC TGTCAAAGCC ATCTCTAGTC 
AAGAGCTTAG CTGGCTCATT TTCTGTGAGT AATGAGGAGA TTTCTCATGA GCATGTTATT 
TTAGAAGCAG CGGATAGCGG TCGTGGAAGT TGGACTTCTT GTTCAAGCAG CTCCCATGAC 
AACTTCCAAA GCCTTCCAAA CCCAAAAGGC TGGGATTTTT TGAACTCTTA TCGACATACT 
CATTTGGATG GCCCCATTGC TGAAGTGGAA CCCCCCGACT GTGAGCCCTA TTCTTGCCCT 
AAAGGCTGCT CTAGAACTTG TGGTCAGTGT AAAGGAAACC TGGAGACTAA TCAGATGAGA 
CAGAGTTGGG CATCCTCTAG TTCTCTGTCT GACACATACG AACCAAACTA TGGGACAGTT 
AAACGGAGAG TGTTAGAGAG CATCACACCT GAGTCCTGTG AAAGCTTGGA TCCCAAGGAT 
GGCACTGACC CAGTTTACAA AACTGTTACT TCAAGTACAG AAAAGGGCTT GATTGTGTAC 
TGTGTCACTT CACCCAAGAA GGAGGATAGG TATAGGGAGC CACCTCCCAC TCCTCCAGGA 
TACATGGGGA TTTCTTTAGC GGACCTAAAG GAAGGACCCC ACCTACACCT AAAACCTCCA 
GATTATAGCG TGGCAGTGCA GAGGTCAAAG ATGATGCATG ACAGCCTCTC TAGACTGCCA 
CCCACTTCTC TCGGTAGCAG CCCCGTGGCC TGTGTTCCAT CGAGGATTGT AACTCAGCCT 
CAGAGGCATA TTTTGCAGCC ATCCCATCCT AAACTAGCAG ATGTGACTGA CGCAGATAGC 
GAAGCAGATG AAAATGAACA GGTCTCAGCA GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013973177.1
CDS	894..5309
Translation	MAFLVRCYANCLQPWASKLPADLTKMHITDNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEDEEIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAIILEDGQELDSWYVILNGTVEITHPDGKVENLFMGNSFGITPTLDKQYMHGVVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLESPLDVGIKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFEGDPAMTRFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFSLNGGSEKGFGIFVEGVEPSSKAADAGLKRGDQIMEVNGQNFENITFVKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDIEHTSQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFRRLFCDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKADQQSCYIIISKDTTAKEVVCHAVHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELVKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLDSKTGNTHLKEFEDIVNQETFWVASEILTESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLASVARLRGTWEKLPSKYEKHLQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRQVVRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDVDTDEEKFQMMSLQWEPAYGTLTKNLSEKRSAKSSEMSPVPMRSAGQTAKAHLHQPHRVSQVLQVPAVNLHPIRKKGQAKDPATLSTNLPQKVLGTTEEVSGKKHPEDTISVASSLHSSPPASPQGSPRKGYTLTPSAKSDNLSDSSHSEISSRSSIVSNCSVDSMSTALQDERCSSQAVAVPESTGPLEKTEHSSGIGDHSQLGPGWTLSKPSLVKSLAGSFSVSNEEISHEHVILEAADSGRGSWTSCSSSSHDNFQSLPNPKGWDFLNSYRHTHLDGPIAEVEPPDCEPYSCPKGCSRTCGQCKGNLETNQMRQSWASSSSLSDTYEPNYGTVKRRVLESITPESCESLDPKDGTDPVYKTVTSSTEKGLIVYCVTSPKKEDRYREPPPTPPGYMGISLADLKEGPHLHLKPPDYSVAVQRSKMMHDSLSRLPPTSLGSSPVACVPSRIVTQPQRHILQPSHPKLADVTDADSEADENEQVSAV

Target ORF information:

RefSeq Version	XM_014117702.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014117702.2

ORF Insert Sequence:

ATGGCTTTTC TTGTTCGGTG TTATGCCAAT TGTCTTCAGC CATGGGCCTC CAAACTTCCA 
GCCGATCTTA CCAAGATGCA CATCACAGAC AACCCTCATC CACAGGTGAC TCATGTGTCT 
TCTAGTCAGT CTGGTTGTAG CATCGCCAGT GACTCTGGGA GCAGCAGTTT ATCTGATATC 
TATCAGGCTA CGGAAAGTGA GGTAGGAGAT GTAGATTTAA CACGTCTTCC AGAAGGACCT 
GTTGATTCTG AAGATGAAGA AGAGGAAGAT GAAGAAATTG ATCGAACGGA TCCACTACAG 
GGGCGAGATC TTGTTCGAGA ATGTCTTGAG AAAGAACCTG CAGACAAAAC TGATGATGAC 
ATTGAACAAT TACTGGAGTT TATGCACCAG CTCCCAGCTT TTGCAAACAT GACCATGTCT 
GTAAGAAGAG AACTCTGCTC AGTGATGATT TTTGAAGTGG TGGAGCAGGC TGGAGCTATT 
ATTCTTGAAG ATGGGCAAGA GCTTGACTCA TGGTATGTTA TTTTAAACGG CACTGTAGAA 
ATCACTCATC CAGATGGAAA AGTTGAAAAT CTCTTTATGG GAAATAGTTT TGGAATTACT 
CCCACTCTGG ACAAACAGTA CATGCATGGA GTTGTTAGGA CGAAAGTAGA TGATTGTCAG 
TTTGTCTGCA TAGCGCAGCA GGATTATTGG AGAATTTTAA ATCATGTGGA GAAAAACACC 
CATAAAGTTG AGGAAGAGGG AGAAATTGTC ATGGTACATG AGCATCGTGA ACTAGATCGG 
AGTGGAACCA GGAAAGGACA CATTGTAATC AAGGCAACAC CTGAGCGTCT CATCATGCAT 
TTAATAGAAG AGCATTCCAT TGTGGACCCG ACTTATATAG AAGATTTCCT GCTAACTTAC 
AGGACATTTC TTGAAAGTCC TTTGGATGTT GGGATCAAAC TATTGGAATG GTTTAAAATT 
GACAGCTTAA GGGATAAGGT AACAAGGATT GTGTTATTAT GGGTAAATAA TCATTTTAAT 
GATTTTGAAG GTGATCCTGC TATGACTCGA TTTCTGGAGG AATTTGAAAA AAATCTGGAA 
GATACGAAAA TGAATGGTCA TCTCCGGTTA TTGAATATTG CCTGTGCTGC AAAGGCCAAG 
TGGAGACAGG TGGTGCTGCA AAAGGCTTCC CGGGAGTCCC CTCTGCATTT CAGCCTTAAT 
GGAGGGAGTG AGAAGGGCTT TGGTATTTTT GTTGAAGGAG TAGAACCTAG TAGCAAAGCT 
GCTGATGCTG GACTGAAACG TGGTGATCAG ATAATGGAAG TAAATGGACA AAACTTTGAA 
AATATTACAT TTGTGAAAGC CCTTGAAATT TTAAGGAATA ATACTCATCT TGCACTTACT 
GTGAAGACCA ACATTTTTGT GTTCAAAGAA TTACTTAGTA GGACTGAACA AGAAAAATCT 
GGTGTTCCTC ATATTCCTAA AATTGCTGAA AAAAAAAGTA ATCGCCATTC AATCCAGGAT 
GTACCGGGAG ATATTGAACA CACATCACAG GAGAAAGGAA ATAAGAAAAT AAAAGCAAAC 
ACTGTTTCAG GTGGAAGAAA CAAAATTAGG AAAATTTTGG ATAAAACACG GTTTAGTATC 
TTACCTCCAA AACTGTTTAG GAGACTGTTT TGTGATGGAG GCCTGAGCCA GTCACAAGAT 
GACAGCATTG TGGGAACAAG ACACTGCAGG CACAGTCTGG CTATCATGCC TATTCCTGGA 
ACACTCTCAT CCAGCAGCCC TGATCTCTTG CAGCCTACCA CCAGCATGTT GGATTTTTCT 
AATCCTTCAG ATATTCCTGA TCAAGTTATA AGAGTTTTCA AAGCAGATCA ACAAAGTTGC 
TACATTATCA TCAGTAAAGA CACTACAGCT AAAGAAGTTG TCTGTCATGC TGTTCATGAA 
TTTGGTTTGA CTGGTGCATC CGACACGTAT TCTCTCTGTG AAGTTTCTGT TACTCCTGAG 
GGTGTCATAA AACAAAGAAG ACTTCCAGAC CAATTCTCCA AATTAGCTGA TAGAATTCAG 
CTCAATGGAA GATATTATTT AAAAAATAAC ATGGAAACAG AAACCCTATG TTCAGATGAA 
GATGCTCAAG AACTAGTTAA GGAAAGCCAA CTATCCATGC TGCAGCTCAG TACCATTGAG 
GTGGCCACTC AGCTGTCAAT GAGGGACTTT GATTTGTTTC GTAATATTGA GCCTACTGAA 
TACATTGATG ACCTTTTTAA GTTAGATTCC AAAACAGGAA ATACTCACTT GAAAGAGTTT 
GAGGACATTG TTAACCAGGA GACATTCTGG GTTGCCTCAG AGATTTTAAC GGAATCAAAT 
CAGTTGAAAC GAATGAAGAT TATTAAGCAT TTTATTAAAA TTGCACTTCA TTGTCGAGAA 
TGTAAGAACT TCAATTCCAT GTTTGCAATA ATAAGTGGCT TGAACCTGGC ATCTGTAGCA 
CGACTCAGAG GTACTTGGGA AAAATTACCA AGCAAGTATG AGAAACACCT TCAAGATCTA 
CAGGACCTTT TTGATCCATC TAGAAACATG GCAAAATATA GAAATATTCT TAGTAGTCAA 
AGCATGCAGC CTCCAATTAT TCCACTCTTC CCTGTTGTCA AGAAAGATAT GACATTTCTA 
CATGAAGGAA ATGACTCCAA AGTAGATGGT TTAGTGAATT TTGAGAAGCT AAGAATGATT 
GCCAAGGAGA TCCGTCAGGT TGTTCGAATG ACTTCTGCTA ACATGGACCC AGCTATGATG 
TTTCGACAGA GGAAGAAGAG GTGGCGGAGT CTGGGGTCAC TGAGTCAAGG CAGCACAAAC 
TCAAACATGC TGGATGTTCA AGGAGGTGCA CACAAGAAAA GGGCACGTCG CAGCTCTCTA 
CTTAATGCCA AGAAGTTATA CGAGGATGCC CAGATGGCAA GGAAAGTGAA GCAGTATCTT 
TCCAGTCTGG ATGTCGACAC AGATGAGGAA AAGTTTCAGA TGATGTCTTT GCAGTGGGAG 
CCTGCCTATG GTACCTTGAC CAAGAATTTA AGTGAGAAAA GATCAGCCAA ATCATCAGAA 
ATGTCTCCAG TGCCTATGAG ATCGGCAGGC CAAACAGCTA AAGCACATTT GCATCAGCCC 
CACCGAGTAA GCCAGGTGCT TCAAGTGCCA GCTGTTAATT TGCACCCCAT CAGGAAGAAG 
GGACAAGCCA AAGACCCTGC CACGTTGAGT ACAAATTTAC CTCAGAAAGT TTTAGGAACA 
ACTGAAGAAG TAAGTGGTAA GAAACATCCA GAAGACACTA TCTCTGTGGC ATCATCCTTA 
CATTCTAGCC CTCCTGCATC TCCTCAAGGT TCCCCTCGCA AAGGTTATAC ACTTACACCA 
TCAGCTAAAT CTGACAACTT GTCTGACTCC AGCCATAGTG AAATTTCTTC ACGATCCAGC 
ATTGTGAGCA ATTGTTCTGT TGACTCCATG TCTACAGCTC TACAGGATGA ACGGTGTTCT 
TCACAGGCTG TGGCGGTCCC TGAATCCACT GGGCCTTTGG AAAAGACAGA ACACTCCTCA 
GGGATAGGAG ACCATAGTCA GCTTGGCCCC GGGTGGACGC TGTCAAAGCC ATCTCTAGTC 
AAGAGCTTAG CTGGCTCATT TTCTGTGAGT AATGAGGAGA TTTCTCATGA GCATGTTATT 
TTAGAAGCAG CGGATAGCGG TCGTGGAAGT TGGACTTCTT GTTCAAGCAG CTCCCATGAC 
AACTTCCAAA GCCTTCCAAA CCCAAAAGGC TGGGATTTTT TGAACTCTTA TCGACATACT 
CATTTGGATG GCCCCATTGC TGAAGTGGAA CCCCCCGACT GTGAGCCCTA TTCTTGCCCT 
AAAGGCTGCT CTAGAACTTG TGGTCAGTGT AAAGGAAACC TGGAGACTAA TCAGATGAGA 
CAGAGTTGGG CATCCTCTAG TTCTCTGTCT GACACATACG AACCAAACTA TGGGACAGTT 
AAACGGAGAG TGTTAGAGAG CATCACACCT GAGTCCTGTG AAAGCTTGGA TCCCAAGGAT 
GGCACTGACC CAGTTTACAA AACTGTTACT TCAAGTACAG AAAAGGGCTT GATTGTGTAC 
TGTGTCACTT CACCCAAGAA GGAGGATAGG TATAGGGAGC CACCTCCCAC TCCTCCAGGA 
TACATGGGGA TTTCTTTAGC GGACCTAAAG GAAGGACCCC ACCTACACCT AAAACCTCCA 
GATTATAGCG TGGCAGTGCA GAGGTCAAAG ATGATGCATG ACAGCCTCTC TAGACTGCCA 
CCCACTTCTC TCGGTAGCAG CCCCGTGGCC TGTGTTCCAT CGAGGATTGT AACTCAGCCT 
CAGAGGCATA TTTTGCAGCC ATCCCATCCT AAACTAGCAG ATGTGACTGA CGCAGATAGC 
GAAGCAGATG AAAATGAACA GGTCTCAGCA GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( XM_014117703.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013973178.1 Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 6 (RAPGEF6), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OCe39112
Clone ID Related Accession (Same CDS sequence)	XM_014117703.1 , XM_014117703.2
Accession Version	XM_014117703.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	rap guanine nucleotide exchange factor 6 isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCAC CCGTGGACCC CGGCGCTAGG CAGGCGCTGA AGAAGAAGCC TCCCGAGCGG 
ACCCCCGAGG ATTTAAATAC TATTTATTCT TATCTTCATG GGATGGAAAT ACTATCAAAT 
CTCAGGGAGC ATCAGCTTAG ATTAATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGT 
AATCAAATTC TTTTTTGTTC AGAAACTGTT GCCAGATGTT GGTATATTCT GCTTTCTGGA 
TCTGTGCTTA TGAAAGACTC CATGGTCTTG CCTCCATGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGATAG TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGGCGA 
AGGTTTCGGA AAATTAATTA TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGACATT 
AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATCACAGA CAACCCTCAT 
CCACAGGTGA CTCATGTGTC TTCTAGTCAG TCTGGTTGTA GCATCGCCAG TGACTCTGGG 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACGGAAAGTG AGGTAGGAGA TGTAGATTTA 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGAAATT 
GATCGAACGG ATCCACTACA GGGGCGAGAT CTTGTTCGAG AATGTCTTGA GAAAGAACCT 
GCAGACAAAA CTGATGATGA CATTGAACAA TTACTGGAGT TTATGCACCA GCTCCCAGCT 
TTTGCAAACA TGACCATGTC TGTAAGAAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTGGAGCAGG CTGGAGCTAT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTT 
ATTTTAAACG GCACTGTAGA AATCACTCAT CCAGATGGAA AAGTTGAAAA TCTCTTTATG 
GGAAATAGTT TTGGAATTAC TCCCACTCTG GACAAACAGT ACATGCATGG AGTTGTTAGG 
ACGAAAGTAG ATGATTGTCA GTTTGTCTGC ATAGCGCAGC AGGATTATTG GAGAATTTTA 
AATCATGTGG AGAAAAACAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT CATGGTACAT 
GAGCATCGTG AACTAGATCG GAGTGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC GACTTATATA 
GAAGATTTCC TGCTAACTTA CAGGACATTT CTTGAAAGTC CTTTGGATGT TGGGATCAAA 
CTATTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACAAGGAT TGTGTTATTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCG ATTTCTGGAG 
GAATTTGAAA AAAATCTGGA AGATACGAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCCAA GTGGAGACAG GTGGTGCTGC AAAAGGCTTC CCGGGAGTCC 
CCTCTGCATT TCAGCCTTAA TGGAGGGAGT GAGAAGGGCT TTGGTATTTT TGTTGAAGGA 
GTAGAACCTA GTAGCAAAGC TGCTGATGCT GGACTGAAAC GTGGTGATCA GATAATGGAA 
GTAAATGGAC AAAACTTTGA AAATATTACA TTTGTGAAAG CCCTTGAAAT TTTAAGGAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTACTTAGT 
AGGACTGAAC AAGAAAAATC TGGTGTTCCT CATATTCCTA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATCCAGGA TGTACCGGGA GATATTGAAC ACACATCACA GGAGAAAGGA 
AATAAGAAAA TAAAAGCAAA CACTGTTTCA GGTGGAAGAA ACAAAATTAG GAAAATTTTG 
GATAAAACAC GGTTTAGTAT CTTACCTCCA AAACTGTTTA GGAGACTGTT TTGTGATGGA 
GGCCTGAGCC AGTCACAAGA TGACAGCATT GTGGGAACAA GACACTGCAG GCACAGTCTG 
GCTATCATGC CTATTCCTGG AACACTCTCA TCCAGCAGCC CTGATCTCTT GCAGCCTACC 
ACCAGCATGT TGGATTTTTC TAATCCTTCA GATATTCCTG ATCAAGTTAT AAGAGTTTTC 
AAAGCAGATC AACAAAGTTG CTACATTATC ATCAGTAAAG ACACTACAGC TAAAGAAGTT 
GTCTGTCATG CTGTTCATGA ATTTGGTTTG ACTGGTGCAT CCGACACGTA TTCTCTCTGT 
GAAGTTTCTG TTACTCCTGA GGGTGTCATA AAACAAAGAA GACTTCCAGA CCAATTCTCC 
AAATTAGCTG ATAGAATTCA GCTCAATGGA AGATATTATT TAAAAAATAA CATGGAAACA 
GAAACCCTAT GTTCAGATGA AGATGCTCAA GAACTAGTTA AGGAAAGCCA ACTATCCATG 
CTGCAGCTCA GTACCATTGA GGTGGCCACT CAGCTGTCAA TGAGGGACTT TGATTTGTTT 
CGTAATATTG AGCCTACTGA ATACATTGAT GACCTTTTTA AGTTAGATTC CAAAACAGGA 
AATACTCACT TGAAAGAGTT TGAGGACATT GTTAACCAGG AGACATTCTG GGTTGCCTCA 
GAGATTTTAA CGGAATCAAA TCAGTTGAAA CGAATGAAGA TTATTAAGCA TTTTATTAAA 
ATTGCACTTC ATTGTCGAGA ATGTAAGAAC TTCAATTCCA TGTTTGCAAT AATAAGTGGC 
TTGAACCTGG CATCTGTAGC ACGACTCAGA GGTACTTGGG AAAAATTACC AAGCAAGTAT 
GAGAAACACC TTCAAGATCT ACAGGACCTT TTTGATCCAT CTAGAAACAT GGCAAAATAT 
AGAAATATTC TTAGTAGTCA AAGCATGCAG CCTCCAATTA TTCCACTCTT CCCTGTTGTC 
AAGAAAGATA TGACATTTCT ACATGAAGGA AATGACTCCA AAGTAGATGG TTTAGTGAAT 
TTTGAGAAGC TAAGAATGAT TGCCAAGGAG ATCCGTCAGG TTGTTCGAAT GACTTCTGCT 
AACATGGACC CAGCTATGAT GTTTCGACAG AGGAAGAAGA GGTGGCGGAG TCTGGGGTCA 
CTGAGTCAAG GCAGCACAAA CTCAAACATG CTGGATGTTC AAGGAGGTGC ACACAAGAAA 
AGGGCACGTC GCAGCTCTCT ACTTAATGCC AAGAAGTTAT ACGAGGATGC CCAGATGGCA 
AGGAAAGTGA AGCAGTATCT TTCCAGTCTG GATGTCGACA CAGATGAGGA AAAGTTTCAG 
ATGATGTCTT TGCAGTGGGA GCCTGCCTAT GGTACCTTGA CCAAGAATTT AAGTGAGAAA 
AGATCAGCCA AATCATCAGA AATGTCTCCA GTGCCTATGA GATCGGCAGG CCAAACAGCT 
AAAGCACATT TGCATCAGCC CCACCGAGTA AGCCAGGTGC TTCAAGTGCC AGCTGTTAAT 
TTGCACCCCA TCAGGAAGAA GGGACAAGCC AAAGACCCTG CCACGTTGAG TACAAATTTA 
CCTCAGAAAG TTTTAGGAAC AACTGAAGAA GTAAGTGGTA AGAAACATCC AGAAGACACT 
ATCTCTGTGG CATCATCCTT ACATTCTAGC CCTCCTGCAT CTCCTCAAGG TTCCCCTCGC 
AAAGTTGGCA GTATCCTATC TGATCTCAGC ATCCTGCTGC GCCTCTCAGG GTCTTCCTCA 
TCCCCTCCCC GTGAGTCCAG CACCAAGATC AGTGGCCAGA GCTGCCCCGG AATAGGTGGG 
GTTTACTTGC AAAAGAAAAT CCTTCAGATT ACCAGGAGCA CAGCCAAAAG AACAGACAGG 
ACCGAGAAGG CCACTGAGGA GAATAGAGAC AGGACAAGTT GTGAGAACAC AGCCAGAAAA 
AGAATGACGT CCCCGTTTAG AAGATTTAGG GAAAGAACTC TTTCAAGGGA GAGACTAGCC 
AACAGCAAAA AAGAGGACGC AGATCACCAT CAAGCTACAG AAAGCTGTGA GAAAGTGAAG 
GATGTTGGGA GCAACATCAA AGATGAGAAA GGGAGTGACA TCTTCAATTC AAATAGCCGA 
GGCAACCGTA ACACTTTAAA CTGCTTCTAT ACGCGATTCA AGTCTAAGAG GAGAAAGACA 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013973178.1
CDS	240..4505
Translation	MNSPVDPGARQALKKKPPERTPEDLNTIYSYLHGMEILSNLREHQLRLMSARARYERYSGNQILFCSETVARCWYILLSGSVLMKDSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENSKDNEDSILQREIPARQSRRRFRKINYKGERQTITDDVDINSYLSLPADLTKMHITDNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEDEEIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAIILEDGQELDSWYVILNGTVEITHPDGKVENLFMGNSFGITPTLDKQYMHGVVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLESPLDVGIKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFEGDPAMTRFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFSLNGGSEKGFGIFVEGVEPSSKAADAGLKRGDQIMEVNGQNFENITFVKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDIEHTSQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFRRLFCDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKADQQSCYIIISKDTTAKEVVCHAVHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELVKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLDSKTGNTHLKEFEDIVNQETFWVASEILTESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLASVARLRGTWEKLPSKYEKHLQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRQVVRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDVDTDEEKFQMMSLQWEPAYGTLTKNLSEKRSAKSSEMSPVPMRSAGQTAKAHLHQPHRVSQVLQVPAVNLHPIRKKGQAKDPATLSTNLPQKVLGTTEEVSGKKHPEDTISVASSLHSSPPASPQGSPRKVGSILSDLSILLRLSGSSSSPPRESSTKISGQSCPGIGGVYLQKKILQITRSTAKRTDRTEKATEENRDRTSCENTARKRMTSPFRRFRERTLSRERLANSKKEDADHHQATESCEKVKDVGSNIKDEKGSDIFNSNSRGNRNTLNCFYTRFKSKRRKTI

Target ORF information:

RefSeq Version	XM_014117703.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 6 (RAPGEF6), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014117703.1

ORF Insert Sequence:

ATGAACTCAC CCGTGGACCC CGGCGCTAGG CAGGCGCTGA AGAAGAAGCC TCCCGAGCGG 
ACCCCCGAGG ATTTAAATAC TATTTATTCT TATCTTCATG GGATGGAAAT ACTATCAAAT 
CTCAGGGAGC ATCAGCTTAG ATTAATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGT 
AATCAAATTC TTTTTTGTTC AGAAACTGTT GCCAGATGTT GGTATATTCT GCTTTCTGGA 
TCTGTGCTTA TGAAAGACTC CATGGTCTTG CCTCCATGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGATAG TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGGCGA 
AGGTTTCGGA AAATTAATTA TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGACATT 
AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATCACAGA CAACCCTCAT 
CCACAGGTGA CTCATGTGTC TTCTAGTCAG TCTGGTTGTA GCATCGCCAG TGACTCTGGG 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACGGAAAGTG AGGTAGGAGA TGTAGATTTA 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGAAATT 
GATCGAACGG ATCCACTACA GGGGCGAGAT CTTGTTCGAG AATGTCTTGA GAAAGAACCT 
GCAGACAAAA CTGATGATGA CATTGAACAA TTACTGGAGT TTATGCACCA GCTCCCAGCT 
TTTGCAAACA TGACCATGTC TGTAAGAAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTGGAGCAGG CTGGAGCTAT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTT 
ATTTTAAACG GCACTGTAGA AATCACTCAT CCAGATGGAA AAGTTGAAAA TCTCTTTATG 
GGAAATAGTT TTGGAATTAC TCCCACTCTG GACAAACAGT ACATGCATGG AGTTGTTAGG 
ACGAAAGTAG ATGATTGTCA GTTTGTCTGC ATAGCGCAGC AGGATTATTG GAGAATTTTA 
AATCATGTGG AGAAAAACAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT CATGGTACAT 
GAGCATCGTG AACTAGATCG GAGTGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC GACTTATATA 
GAAGATTTCC TGCTAACTTA CAGGACATTT CTTGAAAGTC CTTTGGATGT TGGGATCAAA 
CTATTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACAAGGAT TGTGTTATTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCG ATTTCTGGAG 
GAATTTGAAA AAAATCTGGA AGATACGAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCCAA GTGGAGACAG GTGGTGCTGC AAAAGGCTTC CCGGGAGTCC 
CCTCTGCATT TCAGCCTTAA TGGAGGGAGT GAGAAGGGCT TTGGTATTTT TGTTGAAGGA 
GTAGAACCTA GTAGCAAAGC TGCTGATGCT GGACTGAAAC GTGGTGATCA GATAATGGAA 
GTAAATGGAC AAAACTTTGA AAATATTACA TTTGTGAAAG CCCTTGAAAT TTTAAGGAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTACTTAGT 
AGGACTGAAC AAGAAAAATC TGGTGTTCCT CATATTCCTA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATCCAGGA TGTACCGGGA GATATTGAAC ACACATCACA GGAGAAAGGA 
AATAAGAAAA TAAAAGCAAA CACTGTTTCA GGTGGAAGAA ACAAAATTAG GAAAATTTTG 
GATAAAACAC GGTTTAGTAT CTTACCTCCA AAACTGTTTA GGAGACTGTT TTGTGATGGA 
GGCCTGAGCC AGTCACAAGA TGACAGCATT GTGGGAACAA GACACTGCAG GCACAGTCTG 
GCTATCATGC CTATTCCTGG AACACTCTCA TCCAGCAGCC CTGATCTCTT GCAGCCTACC 
ACCAGCATGT TGGATTTTTC TAATCCTTCA GATATTCCTG ATCAAGTTAT AAGAGTTTTC 
AAAGCAGATC AACAAAGTTG CTACATTATC ATCAGTAAAG ACACTACAGC TAAAGAAGTT 
GTCTGTCATG CTGTTCATGA ATTTGGTTTG ACTGGTGCAT CCGACACGTA TTCTCTCTGT 
GAAGTTTCTG TTACTCCTGA GGGTGTCATA AAACAAAGAA GACTTCCAGA CCAATTCTCC 
AAATTAGCTG ATAGAATTCA GCTCAATGGA AGATATTATT TAAAAAATAA CATGGAAACA 
GAAACCCTAT GTTCAGATGA AGATGCTCAA GAACTAGTTA AGGAAAGCCA ACTATCCATG 
CTGCAGCTCA GTACCATTGA GGTGGCCACT CAGCTGTCAA TGAGGGACTT TGATTTGTTT 
CGTAATATTG AGCCTACTGA ATACATTGAT GACCTTTTTA AGTTAGATTC CAAAACAGGA 
AATACTCACT TGAAAGAGTT TGAGGACATT GTTAACCAGG AGACATTCTG GGTTGCCTCA 
GAGATTTTAA CGGAATCAAA TCAGTTGAAA CGAATGAAGA TTATTAAGCA TTTTATTAAA 
ATTGCACTTC ATTGTCGAGA ATGTAAGAAC TTCAATTCCA TGTTTGCAAT AATAAGTGGC 
TTGAACCTGG CATCTGTAGC ACGACTCAGA GGTACTTGGG AAAAATTACC AAGCAAGTAT 
GAGAAACACC TTCAAGATCT ACAGGACCTT TTTGATCCAT CTAGAAACAT GGCAAAATAT 
AGAAATATTC TTAGTAGTCA AAGCATGCAG CCTCCAATTA TTCCACTCTT CCCTGTTGTC 
AAGAAAGATA TGACATTTCT ACATGAAGGA AATGACTCCA AAGTAGATGG TTTAGTGAAT 
TTTGAGAAGC TAAGAATGAT TGCCAAGGAG ATCCGTCAGG TTGTTCGAAT GACTTCTGCT 
AACATGGACC CAGCTATGAT GTTTCGACAG AGGAAGAAGA GGTGGCGGAG TCTGGGGTCA 
CTGAGTCAAG GCAGCACAAA CTCAAACATG CTGGATGTTC AAGGAGGTGC ACACAAGAAA 
AGGGCACGTC GCAGCTCTCT ACTTAATGCC AAGAAGTTAT ACGAGGATGC CCAGATGGCA 
AGGAAAGTGA AGCAGTATCT TTCCAGTCTG GATGTCGACA CAGATGAGGA AAAGTTTCAG 
ATGATGTCTT TGCAGTGGGA GCCTGCCTAT GGTACCTTGA CCAAGAATTT AAGTGAGAAA 
AGATCAGCCA AATCATCAGA AATGTCTCCA GTGCCTATGA GATCGGCAGG CCAAACAGCT 
AAAGCACATT TGCATCAGCC CCACCGAGTA AGCCAGGTGC TTCAAGTGCC AGCTGTTAAT 
TTGCACCCCA TCAGGAAGAA GGGACAAGCC AAAGACCCTG CCACGTTGAG TACAAATTTA 
CCTCAGAAAG TTTTAGGAAC AACTGAAGAA GTAAGTGGTA AGAAACATCC AGAAGACACT 
ATCTCTGTGG CATCATCCTT ACATTCTAGC CCTCCTGCAT CTCCTCAAGG TTCCCCTCGC 
AAAGTTGGCA GTATCCTATC TGATCTCAGC ATCCTGCTGC GCCTCTCAGG GTCTTCCTCA 
TCCCCTCCCC GTGAGTCCAG CACCAAGATC AGTGGCCAGA GCTGCCCCGG AATAGGTGGG 
GTTTACTTGC AAAAGAAAAT CCTTCAGATT ACCAGGAGCA CAGCCAAAAG AACAGACAGG 
ACCGAGAAGG CCACTGAGGA GAATAGAGAC AGGACAAGTT GTGAGAACAC AGCCAGAAAA 
AGAATGACGT CCCCGTTTAG AAGATTTAGG GAAAGAACTC TTTCAAGGGA GAGACTAGCC 
AACAGCAAAA AAGAGGACGC AGATCACCAT CAAGCTACAG AAAGCTGTGA GAAAGTGAAG 
GATGTTGGGA GCAACATCAA AGATGAGAAA GGGAGTGACA TCTTCAATTC AAATAGCCGA 
GGCAACCGTA ACACTTTAAA CTGCTTCTAT ACGCGATTCA AGTCTAAGAG GAGAAAGACA 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( XM_014117703.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013973178.1 Canis lupus familiaris Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X10, mRNA.

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CloneID	OCe39112
Clone ID Related Accession (Same CDS sequence)	XM_014117703.1 , XM_014117703.2
Accession Version	XM_014117703.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	rap guanine nucleotide exchange factor 6 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014117703.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCAC CCGTGGACCC CGGCGCTAGG CAGGCGCTGA AGAAGAAGCC TCCCGAGCGG 
ACCCCCGAGG ATTTAAATAC TATTTATTCT TATCTTCATG GGATGGAAAT ACTATCAAAT 
CTCAGGGAGC ATCAGCTTAG ATTAATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGT 
AATCAAATTC TTTTTTGTTC AGAAACTGTT GCCAGATGTT GGTATATTCT GCTTTCTGGA 
TCTGTGCTTA TGAAAGACTC CATGGTCTTG CCTCCATGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGATAG TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGGCGA 
AGGTTTCGGA AAATTAATTA TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGACATT 
AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATCACAGA CAACCCTCAT 
CCACAGGTGA CTCATGTGTC TTCTAGTCAG TCTGGTTGTA GCATCGCCAG TGACTCTGGG 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACGGAAAGTG AGGTAGGAGA TGTAGATTTA 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGAAATT 
GATCGAACGG ATCCACTACA GGGGCGAGAT CTTGTTCGAG AATGTCTTGA GAAAGAACCT 
GCAGACAAAA CTGATGATGA CATTGAACAA TTACTGGAGT TTATGCACCA GCTCCCAGCT 
TTTGCAAACA TGACCATGTC TGTAAGAAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTGGAGCAGG CTGGAGCTAT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTT 
ATTTTAAACG GCACTGTAGA AATCACTCAT CCAGATGGAA AAGTTGAAAA TCTCTTTATG 
GGAAATAGTT TTGGAATTAC TCCCACTCTG GACAAACAGT ACATGCATGG AGTTGTTAGG 
ACGAAAGTAG ATGATTGTCA GTTTGTCTGC ATAGCGCAGC AGGATTATTG GAGAATTTTA 
AATCATGTGG AGAAAAACAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT CATGGTACAT 
GAGCATCGTG AACTAGATCG GAGTGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC GACTTATATA 
GAAGATTTCC TGCTAACTTA CAGGACATTT CTTGAAAGTC CTTTGGATGT TGGGATCAAA 
CTATTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACAAGGAT TGTGTTATTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCG ATTTCTGGAG 
GAATTTGAAA AAAATCTGGA AGATACGAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCCAA GTGGAGACAG GTGGTGCTGC AAAAGGCTTC CCGGGAGTCC 
CCTCTGCATT TCAGCCTTAA TGGAGGGAGT GAGAAGGGCT TTGGTATTTT TGTTGAAGGA 
GTAGAACCTA GTAGCAAAGC TGCTGATGCT GGACTGAAAC GTGGTGATCA GATAATGGAA 
GTAAATGGAC AAAACTTTGA AAATATTACA TTTGTGAAAG CCCTTGAAAT TTTAAGGAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTACTTAGT 
AGGACTGAAC AAGAAAAATC TGGTGTTCCT CATATTCCTA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATCCAGGA TGTACCGGGA GATATTGAAC ACACATCACA GGAGAAAGGA 
AATAAGAAAA TAAAAGCAAA CACTGTTTCA GGTGGAAGAA ACAAAATTAG GAAAATTTTG 
GATAAAACAC GGTTTAGTAT CTTACCTCCA AAACTGTTTA GGAGACTGTT TTGTGATGGA 
GGCCTGAGCC AGTCACAAGA TGACAGCATT GTGGGAACAA GACACTGCAG GCACAGTCTG 
GCTATCATGC CTATTCCTGG AACACTCTCA TCCAGCAGCC CTGATCTCTT GCAGCCTACC 
ACCAGCATGT TGGATTTTTC TAATCCTTCA GATATTCCTG ATCAAGTTAT AAGAGTTTTC 
AAAGCAGATC AACAAAGTTG CTACATTATC ATCAGTAAAG ACACTACAGC TAAAGAAGTT 
GTCTGTCATG CTGTTCATGA ATTTGGTTTG ACTGGTGCAT CCGACACGTA TTCTCTCTGT 
GAAGTTTCTG TTACTCCTGA GGGTGTCATA AAACAAAGAA GACTTCCAGA CCAATTCTCC 
AAATTAGCTG ATAGAATTCA GCTCAATGGA AGATATTATT TAAAAAATAA CATGGAAACA 
GAAACCCTAT GTTCAGATGA AGATGCTCAA GAACTAGTTA AGGAAAGCCA ACTATCCATG 
CTGCAGCTCA GTACCATTGA GGTGGCCACT CAGCTGTCAA TGAGGGACTT TGATTTGTTT 
CGTAATATTG AGCCTACTGA ATACATTGAT GACCTTTTTA AGTTAGATTC CAAAACAGGA 
AATACTCACT TGAAAGAGTT TGAGGACATT GTTAACCAGG AGACATTCTG GGTTGCCTCA 
GAGATTTTAA CGGAATCAAA TCAGTTGAAA CGAATGAAGA TTATTAAGCA TTTTATTAAA 
ATTGCACTTC ATTGTCGAGA ATGTAAGAAC TTCAATTCCA TGTTTGCAAT AATAAGTGGC 
TTGAACCTGG CATCTGTAGC ACGACTCAGA GGTACTTGGG AAAAATTACC AAGCAAGTAT 
GAGAAACACC TTCAAGATCT ACAGGACCTT TTTGATCCAT CTAGAAACAT GGCAAAATAT 
AGAAATATTC TTAGTAGTCA AAGCATGCAG CCTCCAATTA TTCCACTCTT CCCTGTTGTC 
AAGAAAGATA TGACATTTCT ACATGAAGGA AATGACTCCA AAGTAGATGG TTTAGTGAAT 
TTTGAGAAGC TAAGAATGAT TGCCAAGGAG ATCCGTCAGG TTGTTCGAAT GACTTCTGCT 
AACATGGACC CAGCTATGAT GTTTCGACAG AGGAAGAAGA GGTGGCGGAG TCTGGGGTCA 
CTGAGTCAAG GCAGCACAAA CTCAAACATG CTGGATGTTC AAGGAGGTGC ACACAAGAAA 
AGGGCACGTC GCAGCTCTCT ACTTAATGCC AAGAAGTTAT ACGAGGATGC CCAGATGGCA 
AGGAAAGTGA AGCAGTATCT TTCCAGTCTG GATGTCGACA CAGATGAGGA AAAGTTTCAG 
ATGATGTCTT TGCAGTGGGA GCCTGCCTAT GGTACCTTGA CCAAGAATTT AAGTGAGAAA 
AGATCAGCCA AATCATCAGA AATGTCTCCA GTGCCTATGA GATCGGCAGG CCAAACAGCT 
AAAGCACATT TGCATCAGCC CCACCGAGTA AGCCAGGTGC TTCAAGTGCC AGCTGTTAAT 
TTGCACCCCA TCAGGAAGAA GGGACAAGCC AAAGACCCTG CCACGTTGAG TACAAATTTA 
CCTCAGAAAG TTTTAGGAAC AACTGAAGAA GTAAGTGGTA AGAAACATCC AGAAGACACT 
ATCTCTGTGG CATCATCCTT ACATTCTAGC CCTCCTGCAT CTCCTCAAGG TTCCCCTCGC 
AAAGTTGGCA GTATCCTATC TGATCTCAGC ATCCTGCTGC GCCTCTCAGG GTCTTCCTCA 
TCCCCTCCCC GTGAGTCCAG CACCAAGATC AGTGGCCAGA GCTGCCCCGG AATAGGTGGG 
GTTTACTTGC AAAAGAAAAT CCTTCAGATT ACCAGGAGCA CAGCCAAAAG AACAGACAGG 
ACCGAGAAGG CCACTGAGGA GAATAGAGAC AGGACAAGTT GTGAGAACAC AGCCAGAAAA 
AGAATGACGT CCCCGTTTAG AAGATTTAGG GAAAGAACTC TTTCAAGGGA GAGACTAGCC 
AACAGCAAAA AAGAGGACGC AGATCACCAT CAAGCTACAG AAAGCTGTGA GAAAGTGAAG 
GATGTTGGGA GCAACATCAA AGATGAGAAA GGGAGTGACA TCTTCAATTC AAATAGCCGA 
GGCAACCGTA ACACTTTAAA CTGCTTCTAT ACGCGATTCA AGTCTAAGAG GAGAAAGACA 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013973178.1
CDS	201..4466
Translation	MNSPVDPGARQALKKKPPERTPEDLNTIYSYLHGMEILSNLREHQLRLMSARARYERYSGNQILFCSETVARCWYILLSGSVLMKDSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENSKDNEDSILQREIPARQSRRRFRKINYKGERQTITDDVDINSYLSLPADLTKMHITDNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEDEEIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAIILEDGQELDSWYVILNGTVEITHPDGKVENLFMGNSFGITPTLDKQYMHGVVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLESPLDVGIKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFEGDPAMTRFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFSLNGGSEKGFGIFVEGVEPSSKAADAGLKRGDQIMEVNGQNFENITFVKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDIEHTSQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFRRLFCDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKADQQSCYIIISKDTTAKEVVCHAVHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELVKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLDSKTGNTHLKEFEDIVNQETFWVASEILTESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLASVARLRGTWEKLPSKYEKHLQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRQVVRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDVDTDEEKFQMMSLQWEPAYGTLTKNLSEKRSAKSSEMSPVPMRSAGQTAKAHLHQPHRVSQVLQVPAVNLHPIRKKGQAKDPATLSTNLPQKVLGTTEEVSGKKHPEDTISVASSLHSSPPASPQGSPRKVGSILSDLSILLRLSGSSSSPPRESSTKISGQSCPGIGGVYLQKKILQITRSTAKRTDRTEKATEENRDRTSCENTARKRMTSPFRRFRERTLSRERLANSKKEDADHHQATESCEKVKDVGSNIKDEKGSDIFNSNSRGNRNTLNCFYTRFKSKRRKTI

Target ORF information:

RefSeq Version	XM_014117703.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014117703.2

ORF Insert Sequence:

ATGAACTCAC CCGTGGACCC CGGCGCTAGG CAGGCGCTGA AGAAGAAGCC TCCCGAGCGG 
ACCCCCGAGG ATTTAAATAC TATTTATTCT TATCTTCATG GGATGGAAAT ACTATCAAAT 
CTCAGGGAGC ATCAGCTTAG ATTAATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGT 
AATCAAATTC TTTTTTGTTC AGAAACTGTT GCCAGATGTT GGTATATTCT GCTTTCTGGA 
TCTGTGCTTA TGAAAGACTC CATGGTCTTG CCTCCATGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGATAG TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGGCGA 
AGGTTTCGGA AAATTAATTA TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGACATT 
AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATCACAGA CAACCCTCAT 
CCACAGGTGA CTCATGTGTC TTCTAGTCAG TCTGGTTGTA GCATCGCCAG TGACTCTGGG 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACGGAAAGTG AGGTAGGAGA TGTAGATTTA 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGAAATT 
GATCGAACGG ATCCACTACA GGGGCGAGAT CTTGTTCGAG AATGTCTTGA GAAAGAACCT 
GCAGACAAAA CTGATGATGA CATTGAACAA TTACTGGAGT TTATGCACCA GCTCCCAGCT 
TTTGCAAACA TGACCATGTC TGTAAGAAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTGGAGCAGG CTGGAGCTAT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTT 
ATTTTAAACG GCACTGTAGA AATCACTCAT CCAGATGGAA AAGTTGAAAA TCTCTTTATG 
GGAAATAGTT TTGGAATTAC TCCCACTCTG GACAAACAGT ACATGCATGG AGTTGTTAGG 
ACGAAAGTAG ATGATTGTCA GTTTGTCTGC ATAGCGCAGC AGGATTATTG GAGAATTTTA 
AATCATGTGG AGAAAAACAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT CATGGTACAT 
GAGCATCGTG AACTAGATCG GAGTGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC GACTTATATA 
GAAGATTTCC TGCTAACTTA CAGGACATTT CTTGAAAGTC CTTTGGATGT TGGGATCAAA 
CTATTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACAAGGAT TGTGTTATTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCG ATTTCTGGAG 
GAATTTGAAA AAAATCTGGA AGATACGAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCCAA GTGGAGACAG GTGGTGCTGC AAAAGGCTTC CCGGGAGTCC 
CCTCTGCATT TCAGCCTTAA TGGAGGGAGT GAGAAGGGCT TTGGTATTTT TGTTGAAGGA 
GTAGAACCTA GTAGCAAAGC TGCTGATGCT GGACTGAAAC GTGGTGATCA GATAATGGAA 
GTAAATGGAC AAAACTTTGA AAATATTACA TTTGTGAAAG CCCTTGAAAT TTTAAGGAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTACTTAGT 
AGGACTGAAC AAGAAAAATC TGGTGTTCCT CATATTCCTA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATCCAGGA TGTACCGGGA GATATTGAAC ACACATCACA GGAGAAAGGA 
AATAAGAAAA TAAAAGCAAA CACTGTTTCA GGTGGAAGAA ACAAAATTAG GAAAATTTTG 
GATAAAACAC GGTTTAGTAT CTTACCTCCA AAACTGTTTA GGAGACTGTT TTGTGATGGA 
GGCCTGAGCC AGTCACAAGA TGACAGCATT GTGGGAACAA GACACTGCAG GCACAGTCTG 
GCTATCATGC CTATTCCTGG AACACTCTCA TCCAGCAGCC CTGATCTCTT GCAGCCTACC 
ACCAGCATGT TGGATTTTTC TAATCCTTCA GATATTCCTG ATCAAGTTAT AAGAGTTTTC 
AAAGCAGATC AACAAAGTTG CTACATTATC ATCAGTAAAG ACACTACAGC TAAAGAAGTT 
GTCTGTCATG CTGTTCATGA ATTTGGTTTG ACTGGTGCAT CCGACACGTA TTCTCTCTGT 
GAAGTTTCTG TTACTCCTGA GGGTGTCATA AAACAAAGAA GACTTCCAGA CCAATTCTCC 
AAATTAGCTG ATAGAATTCA GCTCAATGGA AGATATTATT TAAAAAATAA CATGGAAACA 
GAAACCCTAT GTTCAGATGA AGATGCTCAA GAACTAGTTA AGGAAAGCCA ACTATCCATG 
CTGCAGCTCA GTACCATTGA GGTGGCCACT CAGCTGTCAA TGAGGGACTT TGATTTGTTT 
CGTAATATTG AGCCTACTGA ATACATTGAT GACCTTTTTA AGTTAGATTC CAAAACAGGA 
AATACTCACT TGAAAGAGTT TGAGGACATT GTTAACCAGG AGACATTCTG GGTTGCCTCA 
GAGATTTTAA CGGAATCAAA TCAGTTGAAA CGAATGAAGA TTATTAAGCA TTTTATTAAA 
ATTGCACTTC ATTGTCGAGA ATGTAAGAAC TTCAATTCCA TGTTTGCAAT AATAAGTGGC 
TTGAACCTGG CATCTGTAGC ACGACTCAGA GGTACTTGGG AAAAATTACC AAGCAAGTAT 
GAGAAACACC TTCAAGATCT ACAGGACCTT TTTGATCCAT CTAGAAACAT GGCAAAATAT 
AGAAATATTC TTAGTAGTCA AAGCATGCAG CCTCCAATTA TTCCACTCTT CCCTGTTGTC 
AAGAAAGATA TGACATTTCT ACATGAAGGA AATGACTCCA AAGTAGATGG TTTAGTGAAT 
TTTGAGAAGC TAAGAATGAT TGCCAAGGAG ATCCGTCAGG TTGTTCGAAT GACTTCTGCT 
AACATGGACC CAGCTATGAT GTTTCGACAG AGGAAGAAGA GGTGGCGGAG TCTGGGGTCA 
CTGAGTCAAG GCAGCACAAA CTCAAACATG CTGGATGTTC AAGGAGGTGC ACACAAGAAA 
AGGGCACGTC GCAGCTCTCT ACTTAATGCC AAGAAGTTAT ACGAGGATGC CCAGATGGCA 
AGGAAAGTGA AGCAGTATCT TTCCAGTCTG GATGTCGACA CAGATGAGGA AAAGTTTCAG 
ATGATGTCTT TGCAGTGGGA GCCTGCCTAT GGTACCTTGA CCAAGAATTT AAGTGAGAAA 
AGATCAGCCA AATCATCAGA AATGTCTCCA GTGCCTATGA GATCGGCAGG CCAAACAGCT 
AAAGCACATT TGCATCAGCC CCACCGAGTA AGCCAGGTGC TTCAAGTGCC AGCTGTTAAT 
TTGCACCCCA TCAGGAAGAA GGGACAAGCC AAAGACCCTG CCACGTTGAG TACAAATTTA 
CCTCAGAAAG TTTTAGGAAC AACTGAAGAA GTAAGTGGTA AGAAACATCC AGAAGACACT 
ATCTCTGTGG CATCATCCTT ACATTCTAGC CCTCCTGCAT CTCCTCAAGG TTCCCCTCGC 
AAAGTTGGCA GTATCCTATC TGATCTCAGC ATCCTGCTGC GCCTCTCAGG GTCTTCCTCA 
TCCCCTCCCC GTGAGTCCAG CACCAAGATC AGTGGCCAGA GCTGCCCCGG AATAGGTGGG 
GTTTACTTGC AAAAGAAAAT CCTTCAGATT ACCAGGAGCA CAGCCAAAAG AACAGACAGG 
ACCGAGAAGG CCACTGAGGA GAATAGAGAC AGGACAAGTT GTGAGAACAC AGCCAGAAAA 
AGAATGACGT CCCCGTTTAG AAGATTTAGG GAAAGAACTC TTTCAAGGGA GAGACTAGCC 
AACAGCAAAA AAGAGGACGC AGATCACCAT CAAGCTACAG AAAGCTGTGA GAAAGTGAAG 
GATGTTGGGA GCAACATCAA AGATGAGAAA GGGAGTGACA TCTTCAATTC AAATAGCCGA 
GGCAACCGTA ACACTTTAAA CTGCTTCTAT ACGCGATTCA AGTCTAAGAG GAGAAAGACA 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( XM_014117697.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013973172.1 Canis lupus familiaris Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X1, mRNA.

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CloneID	OCe39106
Clone ID Related Accession (Same CDS sequence)	XM_014117697.2
Accession Version	XM_014117697.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	rap guanine nucleotide exchange factor 6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014117697.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCAC CCGTGGACCC CGGCGCTAGG CAGGCGCTGA AGAAGAAGCC TCCCGAGCGG 
ACCCCCGAGG ATTTAAATAC TATTTATTCT TATCTTCATG GGATGGAAAT ACTATCAAAT 
CTCAGGGAGC ATCAGCTTAG ATTAATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGT 
AATCAAATTC TTTTTTGTTC AGAAACTGTT GCCAGATGTT GGTATATTCT GCTTTCTGGA 
TCTGTGCTTA TGAAAGACTC CATGGTCTTG CCTCCATGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGATAG TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGGCGA 
AGGTTTCGGA AAATTAATTA TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGACATT 
AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATCACAGA CAACCCTCAT 
CCACAGGTGA CTCATGTGTC TTCTAGTCAG TCTGGTTGTA GCATCGCCAG TGACTCTGGG 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACGGAAAGTG AGGTAGGAGA TGTAGATTTA 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGAAATT 
GATCGAACGG ATCCACTACA GGGGCGAGAT CTTGTTCGAG AATGTCTTGA GAAAGAACCT 
GCAGACAAAA CTGATGATGA CATTGAACAA TTACTGGAGT TTATGCACCA GCTCCCAGCT 
TTTGCAAACA TGACCATGTC TGTAAGAAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTGGAGCAGG CTGGAGCTAT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTT 
ATTTTAAACG GCACTGTAGA AATCACTCAT CCAGATGGAA AAGTTGAAAA TCTCTTTATG 
GGAAATAGTT TTGGAATTAC TCCCACTCTG GACAAACAGT ACATGCATGG AGTTGTTAGG 
ACGAAAGTAG ATGATTGTCA GTTTGTCTGC ATAGCGCAGC AGGATTATTG GAGAATTTTA 
AATCATGTGG AGAAAAACAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT CATGGTACAT 
GAGCATCGTG AACTAGATCG GAGTGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC GACTTATATA 
GAAGATTTCC TGCTAACTTA CAGGACATTT CTTGAAAGTC CTTTGGATGT TGGGATCAAA 
CTATTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACAAGGAT TGTGTTATTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCG ATTTCTGGAG 
GAATTTGAAA AAAATCTGGA AGATACGAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCCAA GTGGAGACAG GTGGTGCTGC AAAAGGCTTC CCGGGAGTCC 
CCTCTGCATT TCAGCCTTAA TGGAGGGAGT GAGAAGGGCT TTGGTATTTT TGTTGAAGGA 
GTAGAACCTA GTAGCAAAGC TGCTGATGCT GGACTGAAAC GTGGTGATCA GATAATGGAA 
GTAAATGGAC AAAACTTTGA AAATATTACA TTTGTGAAAG CCCTTGAAAT TTTAAGGAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTACTTAGT 
AGGACTGAAC AAGAAAAATC TGGTGTTCCT CATATTCCTA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATCCAGGA TGTACCGGGA GATATTGAAC ACACATCACA GGAGAAAGGA 
AATAAGAAAA TAAAAGCAAA CACTGTTTCA GGTGGAAGAA ACAAAATTAG GAAAATTTTG 
GATAAAACAC GGTTTAGTAT CTTACCTCCA AAACTGTTTA GGAGACTGTT TTGTGATGGA 
GGCCTGAGCC AGTCACAAGA TGACAGCATT GTGGGAACAA GACACTGCAG GCACAGTCTG 
GCTATCATGC CTATTCCTGG AACACTCTCA TCCAGCAGCC CTGATCTCTT GCAGCCTACC 
ACCAGCATGT TGGATTTTTC TAATCCTTCA GATATTCCTG ATCAAGTTAT AAGAGTTTTC 
AAAGCAGATC AACAAAGTTG CTACATTATC ATCAGTAAAG ACACTACAGC TAAAGAAGTT 
GTCTGTCATG CTGTTCATGA ATTTGGTTTG ACTGGTGCAT CCGACACGTA TTCTCTCTGT 
GAAGTTTCTG TTACTCCTGA GGGTGTCATA AAACAAAGAA GACTTCCAGA CCAATTCTCC 
AAATTAGCTG ATAGAATTCA GCTCAATGGA AGATATTATT TAAAAAATAA CATGGAAACA 
GAAACCCTAT GTTCAGATGA AGATGCTCAA GAACTAGTTA AGGAAAGCCA ACTATCCATG 
CTGCAGCTCA GTACCATTGA GGTGGCCACT CAGCTGTCAA TGAGGGACTT TGATTTGTTT 
CGTAATATTG AGCCTACTGA ATACATTGAT GACCTTTTTA AGTTAGATTC CAAAACAGGA 
AATACTCACT TGAAAGAGTT TGAGGACATT GTTAACCAGG AGACATTCTG GGTTGCCTCA 
GAGATTTTAA CGGAATCAAA TCAGTTGAAA CGAATGAAGA TTATTAAGCA TTTTATTAAA 
ATTGCACTTC ATTGTCGAGA ATGTAAGAAC TTCAATTCCA TGTTTGCAAT AATAAGTGGC 
TTGAACCTGG CATCTGTAGC ACGACTCAGA GGTACTTGGG AAAAATTACC AAGCAAGTAT 
GAGAAACACC TTCAAGATCT ACAGGACCTT TTTGATCCAT CTAGAAACAT GGCAAAATAT 
AGAAATATTC TTAGTAGTCA AAGCATGCAG CCTCCAATTA TTCCACTCTT CCCTGTTGTC 
AAGAAAGATA TGACATTTCT ACATGAAGGA AATGACTCCA AAGTAGATGG TTTAGTGAAT 
TTTGAGAAGC TAAGAATGAT TGCCAAGGAG ATCCGTCAGG TTGTTCGAAT GACTTCTGCT 
AACATGGACC CAGCTATGAT GTTTCGACAG AGGAAGAAGA GGTGGCGGAG TCTGGGGTCA 
CTGAGTCAAG GCAGCACAAA CTCAAACATG CTGGATGTTC AAGGAGGTGC ACACAAGAAA 
AGGGCACGTC GCAGCTCTCT ACTTAATGCC AAGAAGTTAT ACGAGGATGC CCAGATGGCA 
AGGAAAGTGA AGCAGTATCT TTCCAGTCTG GATGTCGACA CAGATGAGGA AAAGTTTCAG 
ATGATGTCTT TGCAGTGGGA GCCTGCCTAT GGTACCTTGA CCAAGAATTT AAGTGAGAAA 
AGATCAGCCA AATCATCAGA AATGTCTCCA GTGCCTATGA GATCGGCAGG CCAAACAGCT 
AAAGCACATT TGCATCAGCC CCACCGAGTA AGCCAGGTGC TTCAAGTGCC AGCTGTTAAT 
TTGCACCCCA TCAGGAAGAA GGGACAAGCC AAAGACCCTG CCACGTTGAG TACAAATTTA 
CCTCAGAAAG TTTTAGGAAC AACTGAAGAA GTAAGTGGTA AGAAACATCC AGAAGACACT 
ATCTCTGTGG CATCATCCTT ACATTCTAGC CCTCCTGCAT CTCCTCAAGG TTCCCCTCGC 
AAAGGTTATA CACTTACACC ATCAGCTAAA TCTGACAACT TGTCTGACTC CAGCCATAGT 
GAAATTTCTT CACGATCCAG CATTGTGAGC AATTGTTCTG TTGACTCCAT GTCTACAGCT 
CTACAGGATG AACGGTGTTC TTCACAGGCT GTGGCGGTCC CTGAATCCAC TGGGCCTTTG 
GAAAAGACAG AACACTCCTC AGGGATAGGA GACCATAGTC AGCTTGGCCC CGGGTGGACG 
CTGTCAAAGC CATCTCTAGT CAAGAGCTTA GCTGGCTCAT TTTCTGTGAG TAATGAGGAG 
ATTTCTCATG AGCATGTTAT TTTAGAAGCA GCGGATAGCG GTCGTGGAAG TTGGACTTCT 
TGTTCAAGCA GCTCCCATGA CAACTTCCAA AGCCTTCCAA ACCCAAAAGG CTGGGATTTT 
TTGAACTCTT ATCGACATAC TCATTTGGAT GGCCCCATTG CTGAAGTGGA ACCCCCCGAC 
TGTGAGCCCT ATTCTTGCCC TAAAGGCTGC TCTAGAACTT GTGGTCAGTG TAAAGGAAAC 
CTGGAGACTA ATCAGATGAG ACAGAGTTGG GCATCCTCTA GTTCTCTGTC TGACACATAC 
GAACCAAACT ATGGGACAGT TAAACGGAGA GTGTTAGAGA GCATCACACC TGAGTCCTGT 
GAAAGCTTGG ATCCCAAGGA TGGCACTGAC CCAGTTTACA AAACTGTTAC TTCAAGTACA 
GAAAAGGGCT TGATTGTGTA CTGTGTCACT TCACCCAAGA AGGAGGATAG GTATAGGGAG 
CCACCTCCCA CTCCTCCAGG ATACATGGGG ATTTCTTTAG CGGACCTAAA GGAAGGACCC 
CACCTACACC TAAAACCTCC AGATTATAGC GTGGCAGTGC AGAGGTCAAA GATGATGCAT 
GACAGCCTCT CTAGACTGCC ACCCACTTCT CTCGGTAGCA GCCCCGTGGC CTGTGTTCCA 
TCGAGGATTG TAACTCAGCC TCAGAGGCAT ATTTTGCAGC CATCCCATCC TAAACTAGCA 
GATGTGACTG ACGCAGATAG CGAAGCAGAT GAAAATGAAC AGGTCTCAGC AGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013973172.1
CDS	199..5055
Translation	MNSPVDPGARQALKKKPPERTPEDLNTIYSYLHGMEILSNLREHQLRLMSARARYERYSGNQILFCSETVARCWYILLSGSVLMKDSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENSKDNEDSILQREIPARQSRRRFRKINYKGERQTITDDVDINSYLSLPADLTKMHITDNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEDEEIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAIILEDGQELDSWYVILNGTVEITHPDGKVENLFMGNSFGITPTLDKQYMHGVVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLESPLDVGIKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFEGDPAMTRFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFSLNGGSEKGFGIFVEGVEPSSKAADAGLKRGDQIMEVNGQNFENITFVKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDIEHTSQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFRRLFCDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKADQQSCYIIISKDTTAKEVVCHAVHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELVKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLDSKTGNTHLKEFEDIVNQETFWVASEILTESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLASVARLRGTWEKLPSKYEKHLQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRQVVRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDVDTDEEKFQMMSLQWEPAYGTLTKNLSEKRSAKSSEMSPVPMRSAGQTAKAHLHQPHRVSQVLQVPAVNLHPIRKKGQAKDPATLSTNLPQKVLGTTEEVSGKKHPEDTISVASSLHSSPPASPQGSPRKGYTLTPSAKSDNLSDSSHSEISSRSSIVSNCSVDSMSTALQDERCSSQAVAVPESTGPLEKTEHSSGIGDHSQLGPGWTLSKPSLVKSLAGSFSVSNEEISHEHVILEAADSGRGSWTSCSSSSHDNFQSLPNPKGWDFLNSYRHTHLDGPIAEVEPPDCEPYSCPKGCSRTCGQCKGNLETNQMRQSWASSSSLSDTYEPNYGTVKRRVLESITPESCESLDPKDGTDPVYKTVTSSTEKGLIVYCVTSPKKEDRYREPPPTPPGYMGISLADLKEGPHLHLKPPDYSVAVQRSKMMHDSLSRLPPTSLGSSPVACVPSRIVTQPQRHILQPSHPKLADVTDADSEADENEQVSAV

Target ORF information:

RefSeq Version	XM_014117697.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014117697.2

ORF Insert Sequence:

ATGAACTCAC CCGTGGACCC CGGCGCTAGG CAGGCGCTGA AGAAGAAGCC TCCCGAGCGG 
ACCCCCGAGG ATTTAAATAC TATTTATTCT TATCTTCATG GGATGGAAAT ACTATCAAAT 
CTCAGGGAGC ATCAGCTTAG ATTAATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGT 
AATCAAATTC TTTTTTGTTC AGAAACTGTT GCCAGATGTT GGTATATTCT GCTTTCTGGA 
TCTGTGCTTA TGAAAGACTC CATGGTCTTG CCTCCATGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGATAG TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGGCGA 
AGGTTTCGGA AAATTAATTA TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGACATT 
AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATCACAGA CAACCCTCAT 
CCACAGGTGA CTCATGTGTC TTCTAGTCAG TCTGGTTGTA GCATCGCCAG TGACTCTGGG 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACGGAAAGTG AGGTAGGAGA TGTAGATTTA 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGAAATT 
GATCGAACGG ATCCACTACA GGGGCGAGAT CTTGTTCGAG AATGTCTTGA GAAAGAACCT 
GCAGACAAAA CTGATGATGA CATTGAACAA TTACTGGAGT TTATGCACCA GCTCCCAGCT 
TTTGCAAACA TGACCATGTC TGTAAGAAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTGGAGCAGG CTGGAGCTAT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTT 
ATTTTAAACG GCACTGTAGA AATCACTCAT CCAGATGGAA AAGTTGAAAA TCTCTTTATG 
GGAAATAGTT TTGGAATTAC TCCCACTCTG GACAAACAGT ACATGCATGG AGTTGTTAGG 
ACGAAAGTAG ATGATTGTCA GTTTGTCTGC ATAGCGCAGC AGGATTATTG GAGAATTTTA 
AATCATGTGG AGAAAAACAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT CATGGTACAT 
GAGCATCGTG AACTAGATCG GAGTGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC GACTTATATA 
GAAGATTTCC TGCTAACTTA CAGGACATTT CTTGAAAGTC CTTTGGATGT TGGGATCAAA 
CTATTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACAAGGAT TGTGTTATTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCG ATTTCTGGAG 
GAATTTGAAA AAAATCTGGA AGATACGAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCCAA GTGGAGACAG GTGGTGCTGC AAAAGGCTTC CCGGGAGTCC 
CCTCTGCATT TCAGCCTTAA TGGAGGGAGT GAGAAGGGCT TTGGTATTTT TGTTGAAGGA 
GTAGAACCTA GTAGCAAAGC TGCTGATGCT GGACTGAAAC GTGGTGATCA GATAATGGAA 
GTAAATGGAC AAAACTTTGA AAATATTACA TTTGTGAAAG CCCTTGAAAT TTTAAGGAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTACTTAGT 
AGGACTGAAC AAGAAAAATC TGGTGTTCCT CATATTCCTA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATCCAGGA TGTACCGGGA GATATTGAAC ACACATCACA GGAGAAAGGA 
AATAAGAAAA TAAAAGCAAA CACTGTTTCA GGTGGAAGAA ACAAAATTAG GAAAATTTTG 
GATAAAACAC GGTTTAGTAT CTTACCTCCA AAACTGTTTA GGAGACTGTT TTGTGATGGA 
GGCCTGAGCC AGTCACAAGA TGACAGCATT GTGGGAACAA GACACTGCAG GCACAGTCTG 
GCTATCATGC CTATTCCTGG AACACTCTCA TCCAGCAGCC CTGATCTCTT GCAGCCTACC 
ACCAGCATGT TGGATTTTTC TAATCCTTCA GATATTCCTG ATCAAGTTAT AAGAGTTTTC 
AAAGCAGATC AACAAAGTTG CTACATTATC ATCAGTAAAG ACACTACAGC TAAAGAAGTT 
GTCTGTCATG CTGTTCATGA ATTTGGTTTG ACTGGTGCAT CCGACACGTA TTCTCTCTGT 
GAAGTTTCTG TTACTCCTGA GGGTGTCATA AAACAAAGAA GACTTCCAGA CCAATTCTCC 
AAATTAGCTG ATAGAATTCA GCTCAATGGA AGATATTATT TAAAAAATAA CATGGAAACA 
GAAACCCTAT GTTCAGATGA AGATGCTCAA GAACTAGTTA AGGAAAGCCA ACTATCCATG 
CTGCAGCTCA GTACCATTGA GGTGGCCACT CAGCTGTCAA TGAGGGACTT TGATTTGTTT 
CGTAATATTG AGCCTACTGA ATACATTGAT GACCTTTTTA AGTTAGATTC CAAAACAGGA 
AATACTCACT TGAAAGAGTT TGAGGACATT GTTAACCAGG AGACATTCTG GGTTGCCTCA 
GAGATTTTAA CGGAATCAAA TCAGTTGAAA CGAATGAAGA TTATTAAGCA TTTTATTAAA 
ATTGCACTTC ATTGTCGAGA ATGTAAGAAC TTCAATTCCA TGTTTGCAAT AATAAGTGGC 
TTGAACCTGG CATCTGTAGC ACGACTCAGA GGTACTTGGG AAAAATTACC AAGCAAGTAT 
GAGAAACACC TTCAAGATCT ACAGGACCTT TTTGATCCAT CTAGAAACAT GGCAAAATAT 
AGAAATATTC TTAGTAGTCA AAGCATGCAG CCTCCAATTA TTCCACTCTT CCCTGTTGTC 
AAGAAAGATA TGACATTTCT ACATGAAGGA AATGACTCCA AAGTAGATGG TTTAGTGAAT 
TTTGAGAAGC TAAGAATGAT TGCCAAGGAG ATCCGTCAGG TTGTTCGAAT GACTTCTGCT 
AACATGGACC CAGCTATGAT GTTTCGACAG AGGAAGAAGA GGTGGCGGAG TCTGGGGTCA 
CTGAGTCAAG GCAGCACAAA CTCAAACATG CTGGATGTTC AAGGAGGTGC ACACAAGAAA 
AGGGCACGTC GCAGCTCTCT ACTTAATGCC AAGAAGTTAT ACGAGGATGC CCAGATGGCA 
AGGAAAGTGA AGCAGTATCT TTCCAGTCTG GATGTCGACA CAGATGAGGA AAAGTTTCAG 
ATGATGTCTT TGCAGTGGGA GCCTGCCTAT GGTACCTTGA CCAAGAATTT AAGTGAGAAA 
AGATCAGCCA AATCATCAGA AATGTCTCCA GTGCCTATGA GATCGGCAGG CCAAACAGCT 
AAAGCACATT TGCATCAGCC CCACCGAGTA AGCCAGGTGC TTCAAGTGCC AGCTGTTAAT 
TTGCACCCCA TCAGGAAGAA GGGACAAGCC AAAGACCCTG CCACGTTGAG TACAAATTTA 
CCTCAGAAAG TTTTAGGAAC AACTGAAGAA GTAAGTGGTA AGAAACATCC AGAAGACACT 
ATCTCTGTGG CATCATCCTT ACATTCTAGC CCTCCTGCAT CTCCTCAAGG TTCCCCTCGC 
AAAGGTTATA CACTTACACC ATCAGCTAAA TCTGACAACT TGTCTGACTC CAGCCATAGT 
GAAATTTCTT CACGATCCAG CATTGTGAGC AATTGTTCTG TTGACTCCAT GTCTACAGCT 
CTACAGGATG AACGGTGTTC TTCACAGGCT GTGGCGGTCC CTGAATCCAC TGGGCCTTTG 
GAAAAGACAG AACACTCCTC AGGGATAGGA GACCATAGTC AGCTTGGCCC CGGGTGGACG 
CTGTCAAAGC CATCTCTAGT CAAGAGCTTA GCTGGCTCAT TTTCTGTGAG TAATGAGGAG 
ATTTCTCATG AGCATGTTAT TTTAGAAGCA GCGGATAGCG GTCGTGGAAG TTGGACTTCT 
TGTTCAAGCA GCTCCCATGA CAACTTCCAA AGCCTTCCAA ACCCAAAAGG CTGGGATTTT 
TTGAACTCTT ATCGACATAC TCATTTGGAT GGCCCCATTG CTGAAGTGGA ACCCCCCGAC 
TGTGAGCCCT ATTCTTGCCC TAAAGGCTGC TCTAGAACTT GTGGTCAGTG TAAAGGAAAC 
CTGGAGACTA ATCAGATGAG ACAGAGTTGG GCATCCTCTA GTTCTCTGTC TGACACATAC 
GAACCAAACT ATGGGACAGT TAAACGGAGA GTGTTAGAGA GCATCACACC TGAGTCCTGT 
GAAAGCTTGG ATCCCAAGGA TGGCACTGAC CCAGTTTACA AAACTGTTAC TTCAAGTACA 
GAAAAGGGCT TGATTGTGTA CTGTGTCACT TCACCCAAGA AGGAGGATAG GTATAGGGAG 
CCACCTCCCA CTCCTCCAGG ATACATGGGG ATTTCTTTAG CGGACCTAAA GGAAGGACCC 
CACCTACACC TAAAACCTCC AGATTATAGC GTGGCAGTGC AGAGGTCAAA GATGATGCAT 
GACAGCCTCT CTAGACTGCC ACCCACTTCT CTCGGTAGCA GCCCCGTGGC CTGTGTTCCA 
TCGAGGATTG TAACTCAGCC TCAGAGGCAT ATTTTGCAGC CATCCCATCC TAAACTAGCA 
GATGTGACTG ACGCAGATAG CGAAGCAGAT GAAAATGAAC AGGTCTCAGC AGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( XM_014117698.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013973173.1 Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 6 (RAPGEF6), transcript variant X3, mRNA.

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CloneID	OCe39107
Clone ID Related Accession (Same CDS sequence)	XM_014117698.1
Accession Version	XM_014117698.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	rap guanine nucleotide exchange factor 6 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCAC CCGTGGACCC CGGCGCTAGG CAGGCGCTGA AGAAGAAGCC TCCCGAGCGG 
ACCCCCGAGG ATTTAAATAC TATTTATTCT TATCTTCATG GGATGGAAAT ACTATCAAAT 
CTCAGGGAGC ATCAGCTTAG ATTAATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGT 
AATCAAATTC TTTTTTGTTC AGAAACTGTT GCCAGATGTT GGTATATTCT GCTTTCTGGA 
TCTGTGCTTA TGAAAGACTC CATGGTCTTG CCTCCATGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGATAG TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGGCGA 
AGGTTTCGGA AAATTAATTA TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGACATT 
AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATCACAGA CAACCCTCAT 
CCACAGGTGA CTCATGTGTC TTCTAGTCAG TCTGGTTGTA GCATCGCCAG TGACTCTGGG 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACGGAAAGTG AGGTAGGAGA TGTAGATTTA 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGAAATT 
GATCGAACGG ATCCACTACA GGGGCGAGAT CTTGTTCGAG AATGTCTTGA GAAAGAACCT 
GCAGACAAAA CTGATGATGA CATTGAACAA TTACTGGAGT TTATGCACCA GCTCCCAGCT 
TTTGCAAACA TGACCATGTC TGTAAGAAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTGGAGCAGG CTGGAGCTAT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTT 
ATTTTAAACG GCACTGTAGA AATCACTCAT CCAGATGGAA AAGTTGAAAA TCTCTTTATG 
GGAAATAGTT TTGGAATTAC TCCCACTCTG GACAAACAGT ACATGCATGG AGTTGTTAGG 
ACGAAAGTAG ATGATTGTCA GTTTGTCTGC ATAGCGCAGC AGGATTATTG GAGAATTTTA 
AATCATGTGG AGAAAAACAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT CATGGTACAT 
GAGCATCGTG AACTAGATCG GAGTGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC GACTTATATA 
GAAGATTTCC TGCTAACTTA CAGGACATTT CTTGAAAGTC CTTTGGATGT TGGGATCAAA 
CTATTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACAAGGAT TGTGTTATTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCG ATTTCTGGAG 
GAATTTGAAA AAAATCTGGA AGATACGAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCCAA GTGGAGACAG GTGGTGCTGC AAAAGGCTTC CCGGGAGTCC 
CCTCTGCATT TCAGCCTTAA TGGAGGGAGT GAGAAGGGCT TTGGTATTTT TGTTGAAGGA 
GTAGAACCTA GTAGCAAAGC TGCTGATGCT GGACTGAAAC GTGGTGATCA GATAATGGAA 
GTAAATGGAC AAAACTTTGA AAATATTACA TTTGTGAAAG CCCTTGAAAT TTTAAGGAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTACTTAGT 
AGGACTGAAC AAGAAAAATC TGGTGTTCCT CATATTCCTA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATCCAGGA TGTACCGGGA GATATTGAAC ACACATCACA GGAGAAAGGA 
AATAAGAAAA TAAAAGCAAA CACTGTTTCA GGTGGAAGAA ACAAAATTAG GAAAATTTTG 
GATAAAACAC GGTTTAGTAT CTTACCTCCA AAACTGTTTA GGAGACTGTT TTGTGATGGA 
GGCCTGAGCC AGTCACAAGA TGACAGCATT GTGGGAACAA GACACTGCAG GCACAGTCTG 
GCTATCATGC CTATTCCTGG AACACTCTCA TCCAGCAGCC CTGATCTCTT GCAGCCTACC 
ACCAGCATGT TGGATTTTTC TAATCCTTCA GATATTCCTG ATCAAGTTAT AAGAGTTTTC 
AAAGCAGATC AACAAAGTTG CTACATTATC ATCAGTAAAG ACACTACAGC TAAAGAAGTT 
GTCTGTCATG CTGTTCATGA ATTTGGTTTG ACTGGTGCAT CCGACACGTA TTCTCTCTGT 
GAAGTTTCTG TTACTCCTGA GGGTGTCATA AAACAAAGAA GACTTCCAGA CCAATTCTCC 
AAATTAGCTG ATAGAATTCA GCTCAATGGA AGATATTATT TAAAAAATAA CATGGAAACA 
GAAACCCTAT GTTCAGATGA AGATGCTCAA GAACTAGTTA AGGAAAGCCA ACTATCCATG 
CTGCAGCTCA GTACCATTGA GGTGGCCACT CAGCTGTCAA TGAGGGACTT TGATTTGTTT 
CGTAATATTG AGCCTACTGA ATACATTGAT GACCTTTTTA AGTTAGATTC CAAAACAGGA 
AATACTCACT TGAAAGAGTT TGAGGACATT GTTAACCAGG AGACATTCTG GGTTGCCTCA 
GAGATTTTAA CGGAATCAAA TCAGTTGAAA CGAATGAAGA TTATTAAGCA TTTTATTAAA 
ATTGCACTTC ATTGTCGAGA ATGTAAGAAC TTCAATTCCA TGTTTGCAAT AATAAGTGGC 
TTGAACCTGG CATCTGTAGC ACGACTCAGA GGTACTTGGG AAAAATTACC AAGCAAGTAT 
GAGAAACACC TTCAAGATCT ACAGGACCTT TTTGATCCAT CTAGAAACAT GGCAAAATAT 
AGAAATATTC TTAGTAGTCA AAGCATGCAG CCTCCAATTA TTCCACTCTT CCCTGTTGTC 
AAGAAAGATA TGACATTTCT ACATGAAGGA AATGACTCCA AAGTAGATGG TTTAGTGAAT 
TTTGAGAAGC TAAGAATGAT TGCCAAGGAG ATCCGTCAGG TTGTTCGAAT GACTTCTGCT 
AACATGGACC CAGCTATGAT GTTTCGACAG AGGTCACTGA GTCAAGGCAG CACAAACTCA 
AACATGCTGG ATGTTCAAGG AGGTGCACAC AAGAAAAGGG CACGTCGCAG CTCTCTACTT 
AATGCCAAGA AGTTATACGA GGATGCCCAG ATGGCAAGGA AAGTGAAGCA GTATCTTTCC 
AGTCTGGATG TCGACACAGA TGAGGAAAAG TTTCAGATGA TGTCTTTGCA GTGGGAGCCT 
GCCTATGGTA CCTTGACCAA GAATTTAAGT GAGAAAAGAT CAGCCAAATC ATCAGAAATG 
TCTCCAGTGC CTATGAGATC GGCAGGCCAA ACAGCTAAAG CACATTTGCA TCAGCCCCAC 
CGAGTAAGCC AGGTGCTTCA AGTGCCAGCT GTTAATTTGC ACCCCATCAG GAAGAAGGGA 
CAAGCCAAAG ACCCTGCCAC GTTGAGTACA AATTTACCTC AGAAAGTTTT AGGAACAACT 
GAAGAAGTAA GTGGTAAGAA ACATCCAGAA GACACTATCT CTGTGGCATC ATCCTTACAT 
TCTAGCCCTC CTGCATCTCC TCAAGGTTCC CCTCGCAAAG GTTATACACT TACACCATCA 
GCTAAATCTG ACAACTTGTC TGACTCCAGC CATAGTGAAA TTTCTTCACG ATCCAGCATT 
GTGAGCAATT GTTCTGTTGA CTCCATGTCT ACAGCTCTAC AGGATGAACG GTGTTCTTCA 
CAGGCTGTGG CGGTCCCTGA ATCCACTGGG CCTTTGGAAA AGACAGAACA CTCCTCAGGG 
ATAGGAGACC ATAGTCAGCT TGGCCCCGGG TGGACGCTGT CAAAGCCATC TCTAGTCAAG 
AGCTTAGCTG GCTCATTTTC TGTGAGTAAT GAGGAGATTT CTCATGAGCA TGTTATTTTA 
GAAGCAGCGG ATAGCGGTCG TGGAAGTTGG ACTTCTTGTT CAAGCAGCTC CCATGACAAC 
TTCCAAAGCC TTCCAAACCC AAAAGGCTGG GATTTTTTGA ACTCTTATCG ACATACTCAT 
TTGGATGGCC CCATTGCTGA AGTGGAACCC CCCGACTGTG AGCCCTATTC TTGCCCTAAA 
GGCTGCTCTA GAACTTGTGG TCAGTGTAAA GGAAACCTGG AGACTAATCA GATGAGACAG 
AGTTGGGCAT CCTCTAGTTC TCTGTCTGAC ACATACGAAC CAAACTATGG GACAGTTAAA 
CGGAGAGTGT TAGAGAGCAT CACACCTGAG TCCTGTGAAA GCTTGGATCC CAAGGATGGC 
ACTGACCCAG TTTACAAAAC TGTTACTTCA AGTACAGAAA AGGGCTTGAT TGTGTACTGT 
GTCACTTCAC CCAAGAAGGA GGATAGGTAT AGGGAGCCAC CTCCCACTCC TCCAGGATAC 
ATGGGGATTT CTTTAGCGGA CCTAAAGGAA GGACCCCACC TACACCTAAA ACCTCCAGAT 
TATAGCGTGG CAGTGCAGAG GTCAAAGATG ATGCATGACA GCCTCTCTAG ACTGCCACCC 
ACTTCTCTCG GTAGCAGCCC CGTGGCCTGT GTTCCATCGA GGATTGTAAC TCAGCCTCAG 
AGGCATATTT TGCAGCCATC CCATCCTAAA CTAGCAGATG TGACTGACGC AGATAGCGAA 
GCAGATGAAA ATGAACAGGT CTCAGCAGTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013973173.1
CDS	238..5070
Translation	MNSPVDPGARQALKKKPPERTPEDLNTIYSYLHGMEILSNLREHQLRLMSARARYERYSGNQILFCSETVARCWYILLSGSVLMKDSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENSKDNEDSILQREIPARQSRRRFRKINYKGERQTITDDVDINSYLSLPADLTKMHITDNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEDEEIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAIILEDGQELDSWYVILNGTVEITHPDGKVENLFMGNSFGITPTLDKQYMHGVVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLESPLDVGIKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFEGDPAMTRFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFSLNGGSEKGFGIFVEGVEPSSKAADAGLKRGDQIMEVNGQNFENITFVKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDIEHTSQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFRRLFCDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKADQQSCYIIISKDTTAKEVVCHAVHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELVKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLDSKTGNTHLKEFEDIVNQETFWVASEILTESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLASVARLRGTWEKLPSKYEKHLQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRQVVRMTSANMDPAMMFRQRSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDVDTDEEKFQMMSLQWEPAYGTLTKNLSEKRSAKSSEMSPVPMRSAGQTAKAHLHQPHRVSQVLQVPAVNLHPIRKKGQAKDPATLSTNLPQKVLGTTEEVSGKKHPEDTISVASSLHSSPPASPQGSPRKGYTLTPSAKSDNLSDSSHSEISSRSSIVSNCSVDSMSTALQDERCSSQAVAVPESTGPLEKTEHSSGIGDHSQLGPGWTLSKPSLVKSLAGSFSVSNEEISHEHVILEAADSGRGSWTSCSSSSHDNFQSLPNPKGWDFLNSYRHTHLDGPIAEVEPPDCEPYSCPKGCSRTCGQCKGNLETNQMRQSWASSSSLSDTYEPNYGTVKRRVLESITPESCESLDPKDGTDPVYKTVTSSTEKGLIVYCVTSPKKEDRYREPPPTPPGYMGISLADLKEGPHLHLKPPDYSVAVQRSKMMHDSLSRLPPTSLGSSPVACVPSRIVTQPQRHILQPSHPKLADVTDADSEADENEQVSAV

Target ORF information:

RefSeq Version	XM_014117698.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 6 (RAPGEF6), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014117698.1

ORF Insert Sequence:

ATGAACTCAC CCGTGGACCC CGGCGCTAGG CAGGCGCTGA AGAAGAAGCC TCCCGAGCGG 
ACCCCCGAGG ATTTAAATAC TATTTATTCT TATCTTCATG GGATGGAAAT ACTATCAAAT 
CTCAGGGAGC ATCAGCTTAG ATTAATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGT 
AATCAAATTC TTTTTTGTTC AGAAACTGTT GCCAGATGTT GGTATATTCT GCTTTCTGGA 
TCTGTGCTTA TGAAAGACTC CATGGTCTTG CCTCCATGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGATAG TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGGCGA 
AGGTTTCGGA AAATTAATTA TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGACATT 
AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATCACAGA CAACCCTCAT 
CCACAGGTGA CTCATGTGTC TTCTAGTCAG TCTGGTTGTA GCATCGCCAG TGACTCTGGG 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACGGAAAGTG AGGTAGGAGA TGTAGATTTA 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGAAATT 
GATCGAACGG ATCCACTACA GGGGCGAGAT CTTGTTCGAG AATGTCTTGA GAAAGAACCT 
GCAGACAAAA CTGATGATGA CATTGAACAA TTACTGGAGT TTATGCACCA GCTCCCAGCT 
TTTGCAAACA TGACCATGTC TGTAAGAAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTGGAGCAGG CTGGAGCTAT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTT 
ATTTTAAACG GCACTGTAGA AATCACTCAT CCAGATGGAA AAGTTGAAAA TCTCTTTATG 
GGAAATAGTT TTGGAATTAC TCCCACTCTG GACAAACAGT ACATGCATGG AGTTGTTAGG 
ACGAAAGTAG ATGATTGTCA GTTTGTCTGC ATAGCGCAGC AGGATTATTG GAGAATTTTA 
AATCATGTGG AGAAAAACAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT CATGGTACAT 
GAGCATCGTG AACTAGATCG GAGTGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC GACTTATATA 
GAAGATTTCC TGCTAACTTA CAGGACATTT CTTGAAAGTC CTTTGGATGT TGGGATCAAA 
CTATTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACAAGGAT TGTGTTATTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCG ATTTCTGGAG 
GAATTTGAAA AAAATCTGGA AGATACGAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCCAA GTGGAGACAG GTGGTGCTGC AAAAGGCTTC CCGGGAGTCC 
CCTCTGCATT TCAGCCTTAA TGGAGGGAGT GAGAAGGGCT TTGGTATTTT TGTTGAAGGA 
GTAGAACCTA GTAGCAAAGC TGCTGATGCT GGACTGAAAC GTGGTGATCA GATAATGGAA 
GTAAATGGAC AAAACTTTGA AAATATTACA TTTGTGAAAG CCCTTGAAAT TTTAAGGAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTACTTAGT 
AGGACTGAAC AAGAAAAATC TGGTGTTCCT CATATTCCTA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATCCAGGA TGTACCGGGA GATATTGAAC ACACATCACA GGAGAAAGGA 
AATAAGAAAA TAAAAGCAAA CACTGTTTCA GGTGGAAGAA ACAAAATTAG GAAAATTTTG 
GATAAAACAC GGTTTAGTAT CTTACCTCCA AAACTGTTTA GGAGACTGTT TTGTGATGGA 
GGCCTGAGCC AGTCACAAGA TGACAGCATT GTGGGAACAA GACACTGCAG GCACAGTCTG 
GCTATCATGC CTATTCCTGG AACACTCTCA TCCAGCAGCC CTGATCTCTT GCAGCCTACC 
ACCAGCATGT TGGATTTTTC TAATCCTTCA GATATTCCTG ATCAAGTTAT AAGAGTTTTC 
AAAGCAGATC AACAAAGTTG CTACATTATC ATCAGTAAAG ACACTACAGC TAAAGAAGTT 
GTCTGTCATG CTGTTCATGA ATTTGGTTTG ACTGGTGCAT CCGACACGTA TTCTCTCTGT 
GAAGTTTCTG TTACTCCTGA GGGTGTCATA AAACAAAGAA GACTTCCAGA CCAATTCTCC 
AAATTAGCTG ATAGAATTCA GCTCAATGGA AGATATTATT TAAAAAATAA CATGGAAACA 
GAAACCCTAT GTTCAGATGA AGATGCTCAA GAACTAGTTA AGGAAAGCCA ACTATCCATG 
CTGCAGCTCA GTACCATTGA GGTGGCCACT CAGCTGTCAA TGAGGGACTT TGATTTGTTT 
CGTAATATTG AGCCTACTGA ATACATTGAT GACCTTTTTA AGTTAGATTC CAAAACAGGA 
AATACTCACT TGAAAGAGTT TGAGGACATT GTTAACCAGG AGACATTCTG GGTTGCCTCA 
GAGATTTTAA CGGAATCAAA TCAGTTGAAA CGAATGAAGA TTATTAAGCA TTTTATTAAA 
ATTGCACTTC ATTGTCGAGA ATGTAAGAAC TTCAATTCCA TGTTTGCAAT AATAAGTGGC 
TTGAACCTGG CATCTGTAGC ACGACTCAGA GGTACTTGGG AAAAATTACC AAGCAAGTAT 
GAGAAACACC TTCAAGATCT ACAGGACCTT TTTGATCCAT CTAGAAACAT GGCAAAATAT 
AGAAATATTC TTAGTAGTCA AAGCATGCAG CCTCCAATTA TTCCACTCTT CCCTGTTGTC 
AAGAAAGATA TGACATTTCT ACATGAAGGA AATGACTCCA AAGTAGATGG TTTAGTGAAT 
TTTGAGAAGC TAAGAATGAT TGCCAAGGAG ATCCGTCAGG TTGTTCGAAT GACTTCTGCT 
AACATGGACC CAGCTATGAT GTTTCGACAG AGGTCACTGA GTCAAGGCAG CACAAACTCA 
AACATGCTGG ATGTTCAAGG AGGTGCACAC AAGAAAAGGG CACGTCGCAG CTCTCTACTT 
AATGCCAAGA AGTTATACGA GGATGCCCAG ATGGCAAGGA AAGTGAAGCA GTATCTTTCC 
AGTCTGGATG TCGACACAGA TGAGGAAAAG TTTCAGATGA TGTCTTTGCA GTGGGAGCCT 
GCCTATGGTA CCTTGACCAA GAATTTAAGT GAGAAAAGAT CAGCCAAATC ATCAGAAATG 
TCTCCAGTGC CTATGAGATC GGCAGGCCAA ACAGCTAAAG CACATTTGCA TCAGCCCCAC 
CGAGTAAGCC AGGTGCTTCA AGTGCCAGCT GTTAATTTGC ACCCCATCAG GAAGAAGGGA 
CAAGCCAAAG ACCCTGCCAC GTTGAGTACA AATTTACCTC AGAAAGTTTT AGGAACAACT 
GAAGAAGTAA GTGGTAAGAA ACATCCAGAA GACACTATCT CTGTGGCATC ATCCTTACAT 
TCTAGCCCTC CTGCATCTCC TCAAGGTTCC CCTCGCAAAG GTTATACACT TACACCATCA 
GCTAAATCTG ACAACTTGTC TGACTCCAGC CATAGTGAAA TTTCTTCACG ATCCAGCATT 
GTGAGCAATT GTTCTGTTGA CTCCATGTCT ACAGCTCTAC AGGATGAACG GTGTTCTTCA 
CAGGCTGTGG CGGTCCCTGA ATCCACTGGG CCTTTGGAAA AGACAGAACA CTCCTCAGGG 
ATAGGAGACC ATAGTCAGCT TGGCCCCGGG TGGACGCTGT CAAAGCCATC TCTAGTCAAG 
AGCTTAGCTG GCTCATTTTC TGTGAGTAAT GAGGAGATTT CTCATGAGCA TGTTATTTTA 
GAAGCAGCGG ATAGCGGTCG TGGAAGTTGG ACTTCTTGTT CAAGCAGCTC CCATGACAAC 
TTCCAAAGCC TTCCAAACCC AAAAGGCTGG GATTTTTTGA ACTCTTATCG ACATACTCAT 
TTGGATGGCC CCATTGCTGA AGTGGAACCC CCCGACTGTG AGCCCTATTC TTGCCCTAAA 
GGCTGCTCTA GAACTTGTGG TCAGTGTAAA GGAAACCTGG AGACTAATCA GATGAGACAG 
AGTTGGGCAT CCTCTAGTTC TCTGTCTGAC ACATACGAAC CAAACTATGG GACAGTTAAA 
CGGAGAGTGT TAGAGAGCAT CACACCTGAG TCCTGTGAAA GCTTGGATCC CAAGGATGGC 
ACTGACCCAG TTTACAAAAC TGTTACTTCA AGTACAGAAA AGGGCTTGAT TGTGTACTGT 
GTCACTTCAC CCAAGAAGGA GGATAGGTAT AGGGAGCCAC CTCCCACTCC TCCAGGATAC 
ATGGGGATTT CTTTAGCGGA CCTAAAGGAA GGACCCCACC TACACCTAAA ACCTCCAGAT 
TATAGCGTGG CAGTGCAGAG GTCAAAGATG ATGCATGACA GCCTCTCTAG ACTGCCACCC 
ACTTCTCTCG GTAGCAGCCC CGTGGCCTGT GTTCCATCGA GGATTGTAAC TCAGCCTCAG 
AGGCATATTT TGCAGCCATC CCATCCTAAA CTAGCAGATG TGACTGACGC AGATAGCGAA 
GCAGATGAAA ATGAACAGGT CTCAGCAGTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( XM_014117699.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013973174.1 Canis lupus familiaris Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCe39108
Clone ID Related Accession (Same CDS sequence)	XM_014117699.2
Accession Version	XM_014117699.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	rap guanine nucleotide exchange factor 6 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014117699.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCAC CCGTGGACCC CGGCGCTAGG CAGGCGCTGA AGAAGAAGCC TCCCGAGCGG 
ACCCCCGAGG ATTTAAATAC TATTTATTCT TATCTTCATG GGATGGAAAT ACTATCAAAT 
CTCAGGGAGC ATCAGCTTAG ATTAATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGT 
AATCAAATTC TTTTTTGTTC AGAAACTGTT GCCAGATGTT GGTATATTCT GCTTTCTGGA 
TCTGTGCTTA TGAAAGACTC CATGGTCTTG CCTCCATGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GCTTCCAGCC 
GATCTTACCA AGATGCACAT CACAGACAAC CCTCATCCAC AGGTGACTCA TGTGTCTTCT 
AGTCAGTCTG GTTGTAGCAT CGCCAGTGAC TCTGGGAGCA GCAGTTTATC TGATATCTAT 
CAGGCTACGG AAAGTGAGGT AGGAGATGTA GATTTAACAC GTCTTCCAGA AGGACCTGTT 
GATTCTGAAG ATGAAGAAGA GGAAGATGAA GAAATTGATC GAACGGATCC ACTACAGGGG 
CGAGATCTTG TTCGAGAATG TCTTGAGAAA GAACCTGCAG ACAAAACTGA TGATGACATT 
GAACAATTAC TGGAGTTTAT GCACCAGCTC CCAGCTTTTG CAAACATGAC CATGTCTGTA 
AGAAGAGAAC TCTGCTCAGT GATGATTTTT GAAGTGGTGG AGCAGGCTGG AGCTATTATT 
CTTGAAGATG GGCAAGAGCT TGACTCATGG TATGTTATTT TAAACGGCAC TGTAGAAATC 
ACTCATCCAG ATGGAAAAGT TGAAAATCTC TTTATGGGAA ATAGTTTTGG AATTACTCCC 
ACTCTGGACA AACAGTACAT GCATGGAGTT GTTAGGACGA AAGTAGATGA TTGTCAGTTT 
GTCTGCATAG CGCAGCAGGA TTATTGGAGA ATTTTAAATC ATGTGGAGAA AAACACCCAT 
AAAGTTGAGG AAGAGGGAGA AATTGTCATG GTACATGAGC ATCGTGAACT AGATCGGAGT 
GGAACCAGGA AAGGACACAT TGTAATCAAG GCAACACCTG AGCGTCTCAT CATGCATTTA 
ATAGAAGAGC ATTCCATTGT GGACCCGACT TATATAGAAG ATTTCCTGCT AACTTACAGG 
ACATTTCTTG AAAGTCCTTT GGATGTTGGG ATCAAACTAT TGGAATGGTT TAAAATTGAC 
AGCTTAAGGG ATAAGGTAAC AAGGATTGTG TTATTATGGG TAAATAATCA TTTTAATGAT 
TTTGAAGGTG ATCCTGCTAT GACTCGATTT CTGGAGGAAT TTGAAAAAAA TCTGGAAGAT 
ACGAAAATGA ATGGTCATCT CCGGTTATTG AATATTGCCT GTGCTGCAAA GGCCAAGTGG 
AGACAGGTGG TGCTGCAAAA GGCTTCCCGG GAGTCCCCTC TGCATTTCAG CCTTAATGGA 
GGGAGTGAGA AGGGCTTTGG TATTTTTGTT GAAGGAGTAG AACCTAGTAG CAAAGCTGCT 
GATGCTGGAC TGAAACGTGG TGATCAGATA ATGGAAGTAA ATGGACAAAA CTTTGAAAAT 
ATTACATTTG TGAAAGCCCT TGAAATTTTA AGGAATAATA CTCATCTTGC ACTTACTGTG 
AAGACCAACA TTTTTGTGTT CAAAGAATTA CTTAGTAGGA CTGAACAAGA AAAATCTGGT 
GTTCCTCATA TTCCTAAAAT TGCTGAAAAA AAAAGTAATC GCCATTCAAT CCAGGATGTA 
CCGGGAGATA TTGAACACAC ATCACAGGAG AAAGGAAATA AGAAAATAAA AGCAAACACT 
GTTTCAGGTG GAAGAAACAA AATTAGGAAA ATTTTGGATA AAACACGGTT TAGTATCTTA 
CCTCCAAAAC TGTTTAGGAG ACTGTTTTGT GATGGAGGCC TGAGCCAGTC ACAAGATGAC 
AGCATTGTGG GAACAAGACA CTGCAGGCAC AGTCTGGCTA TCATGCCTAT TCCTGGAACA 
CTCTCATCCA GCAGCCCTGA TCTCTTGCAG CCTACCACCA GCATGTTGGA TTTTTCTAAT 
CCTTCAGATA TTCCTGATCA AGTTATAAGA GTTTTCAAAG CAGATCAACA AAGTTGCTAC 
ATTATCATCA GTAAAGACAC TACAGCTAAA GAAGTTGTCT GTCATGCTGT TCATGAATTT 
GGTTTGACTG GTGCATCCGA CACGTATTCT CTCTGTGAAG TTTCTGTTAC TCCTGAGGGT 
GTCATAAAAC AAAGAAGACT TCCAGACCAA TTCTCCAAAT TAGCTGATAG AATTCAGCTC 
AATGGAAGAT ATTATTTAAA AAATAACATG GAAACAGAAA CCCTATGTTC AGATGAAGAT 
GCTCAAGAAC TAGTTAAGGA AAGCCAACTA TCCATGCTGC AGCTCAGTAC CATTGAGGTG 
GCCACTCAGC TGTCAATGAG GGACTTTGAT TTGTTTCGTA ATATTGAGCC TACTGAATAC 
ATTGATGACC TTTTTAAGTT AGATTCCAAA ACAGGAAATA CTCACTTGAA AGAGTTTGAG 
GACATTGTTA ACCAGGAGAC ATTCTGGGTT GCCTCAGAGA TTTTAACGGA ATCAAATCAG 
TTGAAACGAA TGAAGATTAT TAAGCATTTT ATTAAAATTG CACTTCATTG TCGAGAATGT 
AAGAACTTCA ATTCCATGTT TGCAATAATA AGTGGCTTGA ACCTGGCATC TGTAGCACGA 
CTCAGAGGTA CTTGGGAAAA ATTACCAAGC AAGTATGAGA AACACCTTCA AGATCTACAG 
GACCTTTTTG ATCCATCTAG AAACATGGCA AAATATAGAA ATATTCTTAG TAGTCAAAGC 
ATGCAGCCTC CAATTATTCC ACTCTTCCCT GTTGTCAAGA AAGATATGAC ATTTCTACAT 
GAAGGAAATG ACTCCAAAGT AGATGGTTTA GTGAATTTTG AGAAGCTAAG AATGATTGCC 
AAGGAGATCC GTCAGGTTGT TCGAATGACT TCTGCTAACA TGGACCCAGC TATGATGTTT 
CGACAGAGGA AGAAGAGGTG GCGGAGTCTG GGGTCACTGA GTCAAGGCAG CACAAACTCA 
AACATGCTGG ATGTTCAAGG AGGTGCACAC AAGAAAAGGG CACGTCGCAG CTCTCTACTT 
AATGCCAAGA AGTTATACGA GGATGCCCAG ATGGCAAGGA AAGTGAAGCA GTATCTTTCC 
AGTCTGGATG TCGACACAGA TGAGGAAAAG TTTCAGATGA TGTCTTTGCA GTGGGAGCCT 
GCCTATGGTA CCTTGACCAA GAATTTAAGT GAGAAAAGAT CAGCCAAATC ATCAGAAATG 
TCTCCAGTGC CTATGAGATC GGCAGGCCAA ACAGCTAAAG CACATTTGCA TCAGCCCCAC 
CGAGTAAGCC AGGTGCTTCA AGTGCCAGCT GTTAATTTGC ACCCCATCAG GAAGAAGGGA 
CAAGCCAAAG ACCCTGCCAC GTTGAGTACA AATTTACCTC AGAAAGTTTT AGGAACAACT 
GAAGAAGTAA GTGGTAAGAA ACATCCAGAA GACACTATCT CTGTGGCATC ATCCTTACAT 
TCTAGCCCTC CTGCATCTCC TCAAGGTTCC CCTCGCAAAG GTTATACACT TACACCATCA 
GCTAAATCTG ACAACTTGTC TGACTCCAGC CATAGTGAAA TTTCTTCACG ATCCAGCATT 
GTGAGCAATT GTTCTGTTGA CTCCATGTCT ACAGCTCTAC AGGATGAACG GTGTTCTTCA 
CAGGCTGTGG CGGTCCCTGA ATCCACTGGG CCTTTGGAAA AGACAGAACA CTCCTCAGGG 
ATAGGAGACC ATAGTCAGCT TGGCCCCGGG TGGACGCTGT CAAAGCCATC TCTAGTCAAG 
AGCTTAGCTG GCTCATTTTC TGTGAGTAAT GAGGAGATTT CTCATGAGCA TGTTATTTTA 
GAAGCAGCGG ATAGCGGTCG TGGAAGTTGG ACTTCTTGTT CAAGCAGCTC CCATGACAAC 
TTCCAAAGCC TTCCAAACCC AAAAGGCTGG GATTTTTTGA ACTCTTATCG ACATACTCAT 
TTGGATGGCC CCATTGCTGA AGTGGAACCC CCCGACTGTG AGCCCTATTC TTGCCCTAAA 
GGCTGCTCTA GAACTTGTGG TCAGTGTAAA GGAAACCTGG AGACTAATCA GATGAGACAG 
AGTTGGGCAT CCTCTAGTTC TCTGTCTGAC ACATACGAAC CAAACTATGG GACAGTTAAA 
CGGAGAGTGT TAGAGAGCAT CACACCTGAG TCCTGTGAAA GCTTGGATCC CAAGGATGGC 
ACTGACCCAG TTTACAAAAC TGTTACTTCA AGTACAGAAA AGGGCTTGAT TGTGTACTGT 
GTCACTTCAC CCAAGAAGGA GGATAGGTAT AGGGAGCCAC CTCCCACTCC TCCAGGATAC 
ATGGGGATTT CTTTAGCGGA CCTAAAGGAA GGACCCCACC TACACCTAAA ACCTCCAGAT 
TATAGCGTGG CAGTGCAGAG GTCAAAGATG ATGCATGACA GCCTCTCTAG ACTGCCACCC 
ACTTCTCTCG GTAGCAGCCC CGTGGCCTGT GTTCCATCGA GGATTGTAAC TCAGCCTCAG 
AGGCATATTT TGCAGCCATC CCATCCTAAA CTAGCAGATG TGACTGACGC AGATAGCGAA 
GCAGATGAAA ATGAACAGGT CTCAGCAGTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013973174.1
CDS	199..4911
Translation	MNSPVDPGARQALKKKPPERTPEDLNTIYSYLHGMEILSNLREHQLRLMSARARYERYSGNQILFCSETVARCWYILLSGSVLMKDSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVLPADLTKMHITDNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEDEEIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAIILEDGQELDSWYVILNGTVEITHPDGKVENLFMGNSFGITPTLDKQYMHGVVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLESPLDVGIKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFEGDPAMTRFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFSLNGGSEKGFGIFVEGVEPSSKAADAGLKRGDQIMEVNGQNFENITFVKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDIEHTSQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFRRLFCDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKADQQSCYIIISKDTTAKEVVCHAVHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELVKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLDSKTGNTHLKEFEDIVNQETFWVASEILTESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLASVARLRGTWEKLPSKYEKHLQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRQVVRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDVDTDEEKFQMMSLQWEPAYGTLTKNLSEKRSAKSSEMSPVPMRSAGQTAKAHLHQPHRVSQVLQVPAVNLHPIRKKGQAKDPATLSTNLPQKVLGTTEEVSGKKHPEDTISVASSLHSSPPASPQGSPRKGYTLTPSAKSDNLSDSSHSEISSRSSIVSNCSVDSMSTALQDERCSSQAVAVPESTGPLEKTEHSSGIGDHSQLGPGWTLSKPSLVKSLAGSFSVSNEEISHEHVILEAADSGRGSWTSCSSSSHDNFQSLPNPKGWDFLNSYRHTHLDGPIAEVEPPDCEPYSCPKGCSRTCGQCKGNLETNQMRQSWASSSSLSDTYEPNYGTVKRRVLESITPESCESLDPKDGTDPVYKTVTSSTEKGLIVYCVTSPKKEDRYREPPPTPPGYMGISLADLKEGPHLHLKPPDYSVAVQRSKMMHDSLSRLPPTSLGSSPVACVPSRIVTQPQRHILQPSHPKLADVTDADSEADENEQVSAV

Target ORF information:

RefSeq Version	XM_014117699.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014117699.2

ORF Insert Sequence:

ATGAACTCAC CCGTGGACCC CGGCGCTAGG CAGGCGCTGA AGAAGAAGCC TCCCGAGCGG 
ACCCCCGAGG ATTTAAATAC TATTTATTCT TATCTTCATG GGATGGAAAT ACTATCAAAT 
CTCAGGGAGC ATCAGCTTAG ATTAATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGT 
AATCAAATTC TTTTTTGTTC AGAAACTGTT GCCAGATGTT GGTATATTCT GCTTTCTGGA 
TCTGTGCTTA TGAAAGACTC CATGGTCTTG CCTCCATGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GCTTCCAGCC 
GATCTTACCA AGATGCACAT CACAGACAAC CCTCATCCAC AGGTGACTCA TGTGTCTTCT 
AGTCAGTCTG GTTGTAGCAT CGCCAGTGAC TCTGGGAGCA GCAGTTTATC TGATATCTAT 
CAGGCTACGG AAAGTGAGGT AGGAGATGTA GATTTAACAC GTCTTCCAGA AGGACCTGTT 
GATTCTGAAG ATGAAGAAGA GGAAGATGAA GAAATTGATC GAACGGATCC ACTACAGGGG 
CGAGATCTTG TTCGAGAATG TCTTGAGAAA GAACCTGCAG ACAAAACTGA TGATGACATT 
GAACAATTAC TGGAGTTTAT GCACCAGCTC CCAGCTTTTG CAAACATGAC CATGTCTGTA 
AGAAGAGAAC TCTGCTCAGT GATGATTTTT GAAGTGGTGG AGCAGGCTGG AGCTATTATT 
CTTGAAGATG GGCAAGAGCT TGACTCATGG TATGTTATTT TAAACGGCAC TGTAGAAATC 
ACTCATCCAG ATGGAAAAGT TGAAAATCTC TTTATGGGAA ATAGTTTTGG AATTACTCCC 
ACTCTGGACA AACAGTACAT GCATGGAGTT GTTAGGACGA AAGTAGATGA TTGTCAGTTT 
GTCTGCATAG CGCAGCAGGA TTATTGGAGA ATTTTAAATC ATGTGGAGAA AAACACCCAT 
AAAGTTGAGG AAGAGGGAGA AATTGTCATG GTACATGAGC ATCGTGAACT AGATCGGAGT 
GGAACCAGGA AAGGACACAT TGTAATCAAG GCAACACCTG AGCGTCTCAT CATGCATTTA 
ATAGAAGAGC ATTCCATTGT GGACCCGACT TATATAGAAG ATTTCCTGCT AACTTACAGG 
ACATTTCTTG AAAGTCCTTT GGATGTTGGG ATCAAACTAT TGGAATGGTT TAAAATTGAC 
AGCTTAAGGG ATAAGGTAAC AAGGATTGTG TTATTATGGG TAAATAATCA TTTTAATGAT 
TTTGAAGGTG ATCCTGCTAT GACTCGATTT CTGGAGGAAT TTGAAAAAAA TCTGGAAGAT 
ACGAAAATGA ATGGTCATCT CCGGTTATTG AATATTGCCT GTGCTGCAAA GGCCAAGTGG 
AGACAGGTGG TGCTGCAAAA GGCTTCCCGG GAGTCCCCTC TGCATTTCAG CCTTAATGGA 
GGGAGTGAGA AGGGCTTTGG TATTTTTGTT GAAGGAGTAG AACCTAGTAG CAAAGCTGCT 
GATGCTGGAC TGAAACGTGG TGATCAGATA ATGGAAGTAA ATGGACAAAA CTTTGAAAAT 
ATTACATTTG TGAAAGCCCT TGAAATTTTA AGGAATAATA CTCATCTTGC ACTTACTGTG 
AAGACCAACA TTTTTGTGTT CAAAGAATTA CTTAGTAGGA CTGAACAAGA AAAATCTGGT 
GTTCCTCATA TTCCTAAAAT TGCTGAAAAA AAAAGTAATC GCCATTCAAT CCAGGATGTA 
CCGGGAGATA TTGAACACAC ATCACAGGAG AAAGGAAATA AGAAAATAAA AGCAAACACT 
GTTTCAGGTG GAAGAAACAA AATTAGGAAA ATTTTGGATA AAACACGGTT TAGTATCTTA 
CCTCCAAAAC TGTTTAGGAG ACTGTTTTGT GATGGAGGCC TGAGCCAGTC ACAAGATGAC 
AGCATTGTGG GAACAAGACA CTGCAGGCAC AGTCTGGCTA TCATGCCTAT TCCTGGAACA 
CTCTCATCCA GCAGCCCTGA TCTCTTGCAG CCTACCACCA GCATGTTGGA TTTTTCTAAT 
CCTTCAGATA TTCCTGATCA AGTTATAAGA GTTTTCAAAG CAGATCAACA AAGTTGCTAC 
ATTATCATCA GTAAAGACAC TACAGCTAAA GAAGTTGTCT GTCATGCTGT TCATGAATTT 
GGTTTGACTG GTGCATCCGA CACGTATTCT CTCTGTGAAG TTTCTGTTAC TCCTGAGGGT 
GTCATAAAAC AAAGAAGACT TCCAGACCAA TTCTCCAAAT TAGCTGATAG AATTCAGCTC 
AATGGAAGAT ATTATTTAAA AAATAACATG GAAACAGAAA CCCTATGTTC AGATGAAGAT 
GCTCAAGAAC TAGTTAAGGA AAGCCAACTA TCCATGCTGC AGCTCAGTAC CATTGAGGTG 
GCCACTCAGC TGTCAATGAG GGACTTTGAT TTGTTTCGTA ATATTGAGCC TACTGAATAC 
ATTGATGACC TTTTTAAGTT AGATTCCAAA ACAGGAAATA CTCACTTGAA AGAGTTTGAG 
GACATTGTTA ACCAGGAGAC ATTCTGGGTT GCCTCAGAGA TTTTAACGGA ATCAAATCAG 
TTGAAACGAA TGAAGATTAT TAAGCATTTT ATTAAAATTG CACTTCATTG TCGAGAATGT 
AAGAACTTCA ATTCCATGTT TGCAATAATA AGTGGCTTGA ACCTGGCATC TGTAGCACGA 
CTCAGAGGTA CTTGGGAAAA ATTACCAAGC AAGTATGAGA AACACCTTCA AGATCTACAG 
GACCTTTTTG ATCCATCTAG AAACATGGCA AAATATAGAA ATATTCTTAG TAGTCAAAGC 
ATGCAGCCTC CAATTATTCC ACTCTTCCCT GTTGTCAAGA AAGATATGAC ATTTCTACAT 
GAAGGAAATG ACTCCAAAGT AGATGGTTTA GTGAATTTTG AGAAGCTAAG AATGATTGCC 
AAGGAGATCC GTCAGGTTGT TCGAATGACT TCTGCTAACA TGGACCCAGC TATGATGTTT 
CGACAGAGGA AGAAGAGGTG GCGGAGTCTG GGGTCACTGA GTCAAGGCAG CACAAACTCA 
AACATGCTGG ATGTTCAAGG AGGTGCACAC AAGAAAAGGG CACGTCGCAG CTCTCTACTT 
AATGCCAAGA AGTTATACGA GGATGCCCAG ATGGCAAGGA AAGTGAAGCA GTATCTTTCC 
AGTCTGGATG TCGACACAGA TGAGGAAAAG TTTCAGATGA TGTCTTTGCA GTGGGAGCCT 
GCCTATGGTA CCTTGACCAA GAATTTAAGT GAGAAAAGAT CAGCCAAATC ATCAGAAATG 
TCTCCAGTGC CTATGAGATC GGCAGGCCAA ACAGCTAAAG CACATTTGCA TCAGCCCCAC 
CGAGTAAGCC AGGTGCTTCA AGTGCCAGCT GTTAATTTGC ACCCCATCAG GAAGAAGGGA 
CAAGCCAAAG ACCCTGCCAC GTTGAGTACA AATTTACCTC AGAAAGTTTT AGGAACAACT 
GAAGAAGTAA GTGGTAAGAA ACATCCAGAA GACACTATCT CTGTGGCATC ATCCTTACAT 
TCTAGCCCTC CTGCATCTCC TCAAGGTTCC CCTCGCAAAG GTTATACACT TACACCATCA 
GCTAAATCTG ACAACTTGTC TGACTCCAGC CATAGTGAAA TTTCTTCACG ATCCAGCATT 
GTGAGCAATT GTTCTGTTGA CTCCATGTCT ACAGCTCTAC AGGATGAACG GTGTTCTTCA 
CAGGCTGTGG CGGTCCCTGA ATCCACTGGG CCTTTGGAAA AGACAGAACA CTCCTCAGGG 
ATAGGAGACC ATAGTCAGCT TGGCCCCGGG TGGACGCTGT CAAAGCCATC TCTAGTCAAG 
AGCTTAGCTG GCTCATTTTC TGTGAGTAAT GAGGAGATTT CTCATGAGCA TGTTATTTTA 
GAAGCAGCGG ATAGCGGTCG TGGAAGTTGG ACTTCTTGTT CAAGCAGCTC CCATGACAAC 
TTCCAAAGCC TTCCAAACCC AAAAGGCTGG GATTTTTTGA ACTCTTATCG ACATACTCAT 
TTGGATGGCC CCATTGCTGA AGTGGAACCC CCCGACTGTG AGCCCTATTC TTGCCCTAAA 
GGCTGCTCTA GAACTTGTGG TCAGTGTAAA GGAAACCTGG AGACTAATCA GATGAGACAG 
AGTTGGGCAT CCTCTAGTTC TCTGTCTGAC ACATACGAAC CAAACTATGG GACAGTTAAA 
CGGAGAGTGT TAGAGAGCAT CACACCTGAG TCCTGTGAAA GCTTGGATCC CAAGGATGGC 
ACTGACCCAG TTTACAAAAC TGTTACTTCA AGTACAGAAA AGGGCTTGAT TGTGTACTGT 
GTCACTTCAC CCAAGAAGGA GGATAGGTAT AGGGAGCCAC CTCCCACTCC TCCAGGATAC 
ATGGGGATTT CTTTAGCGGA CCTAAAGGAA GGACCCCACC TACACCTAAA ACCTCCAGAT 
TATAGCGTGG CAGTGCAGAG GTCAAAGATG ATGCATGACA GCCTCTCTAG ACTGCCACCC 
ACTTCTCTCG GTAGCAGCCC CGTGGCCTGT GTTCCATCGA GGATTGTAAC TCAGCCTCAG 
AGGCATATTT TGCAGCCATC CCATCCTAAA CTAGCAGATG TGACTGACGC AGATAGCGAA 
GCAGATGAAA ATGAACAGGT CTCAGCAGTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( XM_014117701.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013973176.1 Canis lupus familiaris Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OCe39110
Clone ID Related Accession (Same CDS sequence)	XM_014117701.2
Accession Version	XM_014117701.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4542bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	rap guanine nucleotide exchange factor 6 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014117701.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCAC CCGTGGACCC CGGCGCTAGG CAGGCGCTGA AGAAGAAGCC TCCCGAGCGG 
ACCCCCGAGG ATTTAAATAC TATTTATTCT TATCTTCATG GGATGGAAAT ACTATCAAAT 
CTCAGGGAGC ATCAGCTTAG ATTAATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGT 
AATCAAATTC TTTTTTGTTC AGAAACTGTT GCCAGATGTT GGTATATTCT GCTTTCTGGA 
TCTGTGCTTA TGAAAGACTC CATGGTCTTG CCTCCATGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGATAG TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGGCGA 
AGGTTTCGGA AAATTAATTA TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGACATT 
AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATCACAGA CAACCCTCAT 
CCACAGGTGA CTCATGTGTC TTCTAGTCAG TCTGGTTGTA GCATCGCCAG TGACTCTGGG 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACGGAAAGTG AGGTAGGAGA TGTAGATTTA 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGAAATT 
GATCGAACGG ATCCACTACA GGGGCGAGAT CTTGTTCGAG AATGTCTTGA GAAAGAACCT 
GCAGACAAAA CTGATGATGA CATTGAACAA TTACTGGAGT TTATGCACCA GCTCCCAGCT 
TTTGCAAACA TGACCATGTC TGTAAGAAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTGGAGCAGG CTGGAGCTAT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTT 
ATTTTAAACG GCACTGTAGA AATCACTCAT CCAGATGGAA AAGTTGAAAA TCTCTTTATG 
GGAAATAGTT TTGGAATTAC TCCCACTCTG GACAAACAGT ACATGCATGG AGTTGTTAGG 
ACGAAAGTAG ATGATTGTCA GTTTGTCTGC ATAGCGCAGC AGGATTATTG GAGAATTTTA 
AATCATGTGG AGAAAAACAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT CATGGTACAT 
GAGCATCGTG AACTAGATCG GAGTGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC GACTTATATA 
GAAGATTTCC TGCTAACTTA CAGGACATTT CTTGAAAGTC CTTTGGATGT TGGGATCAAA 
CTATTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACAAGGAT TGTGTTATTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCG ATTTCTGGAG 
GAATTTGAAA AAAATCTGGA AGATACGAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCCAA GTGGAGACAG GTGGTGCTGC AAAAGGCTTC CCGGGAGTCC 
CCTCTGCATT TCAGCCTTAA TGGAGGGAGT GAGAAGGGCT TTGGTATTTT TGTTGAAGGA 
GTAGAACCTA GTAGCAAAGC TGCTGATGCT GGACTGAAAC GTGGTGATCA GATAATGGAA 
GTAAATGGAC AAAACTTTGA AAATATTACA TTTGTGAAAG CCCTTGAAAT TTTAAGGAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTACTTAGT 
AGGACTGAAC AAGAAAAATC TGGTGTTCCT CATATTCCTA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATCCAGGA TGTACCGGGA GATATTGAAC ACACATCACA GGAGAAAGGA 
AATAAGAAAA TAAAAGCAAA CACTGTTTCA GGTGGAAGAA ACAAAATTAG GAAAATTTTG 
GATAAAACAC GGTTTAGTAT CTTACCTCCA AAACTGTTTA GGAGACTGTT TTGTGATGGA 
GGCCTGAGCC AGTCACAAGA TGACAGCATT GTGGGAACAA GACACTGCAG GCACAGTCTG 
GCTATCATGC CTATTCCTGG AACACTCTCA TCCAGCAGCC CTGATCTCTT GCAGCCTACC 
ACCAGCATGT TGGATTTTTC TAATCCTTCA GATATTCCTG ATCAAGTTAT AAGAGTTTTC 
AAAGCAGATC AACAAAGTTG CTACATTATC ATCAGTAAAG ACACTACAGC TAAAGAAGTT 
GTCTGTCATG CTGTTCATGA ATTTGGTTTG ACTGGTGCAT CCGACACGTA TTCTCTCTGT 
GAAGTTTCTG TTACTCCTGA GGGTGTCATA AAACAAAGAA GACTTCCAGA CCAATTCTCC 
AAATTAGCTG ATAGAATTCA GCTCAATGGA AGATATTATT TAAAAAATAA CATGGAAACA 
GAAACCCTAT GTTCAGATGA AGATGCTCAA GAACTAGTTA AGGAAAGCCA ACTATCCATG 
CTGCAGCTCA GTACCATTGA GGTGGCCACT CAGCTGTCAA TGAGGGACTT TGATTTGTTT 
CGTAATATTG AGCCTACTGA ATACATTGAT GACCTTTTTA AGTTAGATTC CAAAACAGGA 
AATACTCACT TGAAAGAGTT TGAGGACATT GTTAACCAGG AGACATTCTG GGTTGCCTCA 
GAGATTTTAA CGGAATCAAA TCAGTTGAAA CGAATGAAGA TTATTAAGCA TTTTATTAAA 
ATTGCACTTC ATTGTCGAGA ATGTAAGAAC TTCAATTCCA TGTTTGCAAT AATAAGTGGC 
TTGAACCTGG CATCTGTAGC ACGACTCAGA GGTACTTGGG AAAAATTACC AAGCAAGTAT 
GAGAAACACC TTCAAGATCT ACAGGACCTT TTTGATCCAT CTAGAAACAT GGCAAAATAT 
AGAAATATTC TTAGTAGTCA AAGCATGCAG CCTCCAATTA TTCCACTCTT CCCTGTTGTC 
AAGAAAGATA TGACATTTCT ACATGAAGGA AATGACTCCA AAGTAGATGG TTTAGTGAAT 
TTTGAGAAGC TAAGAATGAT TGCCAAGGAG ATCCGTCAGG TTGTTCGAAT GACTTCTGCT 
AACATGGACC CAGCTATGAT GTTTCGACAG AGGAAGAAGA GGTGGCGGAG TCTGGGGTCA 
CTGAGTCAAG GCAGCACAAA CTCAAACATG CTGGATGTTC AAGGAGGTGC ACACAAGAAA 
AGGGCACGTC GCAGCTCTCT ACTTAATGCC AAGAAGTTAT ACGAGGATGC CCAGATGGCA 
AGGAAAGTGA AGCAGTATCT TTCCAGTCTG GATGTCGACA CAGATGAGGA AAAGTTTCAG 
ATGATGTCTT TGCAGTGGGA GCCTGCCTAT GGTACCTTGA CCAAGAATTT AAGTGAGAAA 
AGATCAGCCA AATCATCAGA AATGTCTCCA GTGCCTATGA GATCGGCAGG CCAAACAGCT 
AAAGCACATT TGCATCAGCC CCACCGAGTA AGCCAGGTGC TTCAAGTGCC AGCTGTTAAT 
TTGCACCCCA TCAGGAAGAA GGGACAAGCC AAAGACCCTG CCACGTTGAG TACAAATTTA 
CCTCAGAAAG TTTTAGGAAC AACTGAAGAA GTAAGTGGTA AGAAACATCC AGAAGACACT 
ATCTCTGTGG CATCATCCTT ACATTCTAGC CCTCCTGCAT CTCCTCAAGG TTCCCCTCGC 
AAAGGTTATA CACTTACACC ATCAGCTAAA TCTGACAACT TGTCTGACTC CAGCCATAGT 
GAAATTTCTT CACGATCCAG CATTGTGAGC AATTGTTCTG TTGACTCCAT GTCTACAGCT 
CTACAGGATG AACGGTGTTC TTCACAGGCT GTGGCGGTCC CTGAATCCAC TGGGCCTTTG 
GAAAAGACAG AACACTCCTC AGGGATAGGA GACCATAGTC AGCTTGGCCC CGGGTGGACG 
CTGTCAAAGC CATCTCTAGT CAAGAGCTTA GCTGGCTCAT TTTCTGTGAG TAATGAGGAG 
ATTTCTCATG AGCATGTTAT TTTAGAAGCA GCGGATAGCG GTCGTGGAAG TTGGACTTCT 
TGTTCAAGCA GCTCCCATGA CAACTTCCAA AGCCTTCCAA ACCCAAAAGG CTGGGATTTT 
TTGAACTCTT ATCGACATAC TCATTTGGAT GGCCCCATTG CTGAAGTGGA ACCCCCCGAC 
TGTGAGCCCT ATTCTTGCCC TAAAGGCTGC TCTAGAACTT GTGGTCAGTG TAAAGGAAAC 
CTGGAGACTA ATCAGATGAG ACAGAGTTGG GCATCCTCTA GTTCTCTGTC TGACACATAC 
GAACCAAACT ATGGGACAGT TAAACGGAGA GTGTTAGAGA GCATCACACC TGAGTCCTGT 
GAAAGCTTGG ATCCCAAGGA TGGCACTGAC CCAGTTTACA AAACTGTTAC TTCAAGTACA 
GAAAAGGGCT TGATTGAAAA TGAACAGGTC TCAGCAGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013973176.1
CDS	200..4741
Translation	MNSPVDPGARQALKKKPPERTPEDLNTIYSYLHGMEILSNLREHQLRLMSARARYERYSGNQILFCSETVARCWYILLSGSVLMKDSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENSKDNEDSILQREIPARQSRRRFRKINYKGERQTITDDVDINSYLSLPADLTKMHITDNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEDEEIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAIILEDGQELDSWYVILNGTVEITHPDGKVENLFMGNSFGITPTLDKQYMHGVVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLESPLDVGIKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFEGDPAMTRFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFSLNGGSEKGFGIFVEGVEPSSKAADAGLKRGDQIMEVNGQNFENITFVKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDIEHTSQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFRRLFCDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKADQQSCYIIISKDTTAKEVVCHAVHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELVKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLDSKTGNTHLKEFEDIVNQETFWVASEILTESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLASVARLRGTWEKLPSKYEKHLQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRQVVRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDVDTDEEKFQMMSLQWEPAYGTLTKNLSEKRSAKSSEMSPVPMRSAGQTAKAHLHQPHRVSQVLQVPAVNLHPIRKKGQAKDPATLSTNLPQKVLGTTEEVSGKKHPEDTISVASSLHSSPPASPQGSPRKGYTLTPSAKSDNLSDSSHSEISSRSSIVSNCSVDSMSTALQDERCSSQAVAVPESTGPLEKTEHSSGIGDHSQLGPGWTLSKPSLVKSLAGSFSVSNEEISHEHVILEAADSGRGSWTSCSSSSHDNFQSLPNPKGWDFLNSYRHTHLDGPIAEVEPPDCEPYSCPKGCSRTCGQCKGNLETNQMRQSWASSSSLSDTYEPNYGTVKRRVLESITPESCESLDPKDGTDPVYKTVTSSTEKGLIENEQVSAV

Target ORF information:

RefSeq Version	XM_014117701.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014117701.2

ORF Insert Sequence:

ATGAACTCAC CCGTGGACCC CGGCGCTAGG CAGGCGCTGA AGAAGAAGCC TCCCGAGCGG 
ACCCCCGAGG ATTTAAATAC TATTTATTCT TATCTTCATG GGATGGAAAT ACTATCAAAT 
CTCAGGGAGC ATCAGCTTAG ATTAATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGT 
AATCAAATTC TTTTTTGTTC AGAAACTGTT GCCAGATGTT GGTATATTCT GCTTTCTGGA 
TCTGTGCTTA TGAAAGACTC CATGGTCTTG CCTCCATGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGATAG TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGGCGA 
AGGTTTCGGA AAATTAATTA TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGACATT 
AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATCACAGA CAACCCTCAT 
CCACAGGTGA CTCATGTGTC TTCTAGTCAG TCTGGTTGTA GCATCGCCAG TGACTCTGGG 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACGGAAAGTG AGGTAGGAGA TGTAGATTTA 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGAAATT 
GATCGAACGG ATCCACTACA GGGGCGAGAT CTTGTTCGAG AATGTCTTGA GAAAGAACCT 
GCAGACAAAA CTGATGATGA CATTGAACAA TTACTGGAGT TTATGCACCA GCTCCCAGCT 
TTTGCAAACA TGACCATGTC TGTAAGAAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTGGAGCAGG CTGGAGCTAT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTT 
ATTTTAAACG GCACTGTAGA AATCACTCAT CCAGATGGAA AAGTTGAAAA TCTCTTTATG 
GGAAATAGTT TTGGAATTAC TCCCACTCTG GACAAACAGT ACATGCATGG AGTTGTTAGG 
ACGAAAGTAG ATGATTGTCA GTTTGTCTGC ATAGCGCAGC AGGATTATTG GAGAATTTTA 
AATCATGTGG AGAAAAACAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT CATGGTACAT 
GAGCATCGTG AACTAGATCG GAGTGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC GACTTATATA 
GAAGATTTCC TGCTAACTTA CAGGACATTT CTTGAAAGTC CTTTGGATGT TGGGATCAAA 
CTATTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACAAGGAT TGTGTTATTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCG ATTTCTGGAG 
GAATTTGAAA AAAATCTGGA AGATACGAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCCAA GTGGAGACAG GTGGTGCTGC AAAAGGCTTC CCGGGAGTCC 
CCTCTGCATT TCAGCCTTAA TGGAGGGAGT GAGAAGGGCT TTGGTATTTT TGTTGAAGGA 
GTAGAACCTA GTAGCAAAGC TGCTGATGCT GGACTGAAAC GTGGTGATCA GATAATGGAA 
GTAAATGGAC AAAACTTTGA AAATATTACA TTTGTGAAAG CCCTTGAAAT TTTAAGGAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTACTTAGT 
AGGACTGAAC AAGAAAAATC TGGTGTTCCT CATATTCCTA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATCCAGGA TGTACCGGGA GATATTGAAC ACACATCACA GGAGAAAGGA 
AATAAGAAAA TAAAAGCAAA CACTGTTTCA GGTGGAAGAA ACAAAATTAG GAAAATTTTG 
GATAAAACAC GGTTTAGTAT CTTACCTCCA AAACTGTTTA GGAGACTGTT TTGTGATGGA 
GGCCTGAGCC AGTCACAAGA TGACAGCATT GTGGGAACAA GACACTGCAG GCACAGTCTG 
GCTATCATGC CTATTCCTGG AACACTCTCA TCCAGCAGCC CTGATCTCTT GCAGCCTACC 
ACCAGCATGT TGGATTTTTC TAATCCTTCA GATATTCCTG ATCAAGTTAT AAGAGTTTTC 
AAAGCAGATC AACAAAGTTG CTACATTATC ATCAGTAAAG ACACTACAGC TAAAGAAGTT 
GTCTGTCATG CTGTTCATGA ATTTGGTTTG ACTGGTGCAT CCGACACGTA TTCTCTCTGT 
GAAGTTTCTG TTACTCCTGA GGGTGTCATA AAACAAAGAA GACTTCCAGA CCAATTCTCC 
AAATTAGCTG ATAGAATTCA GCTCAATGGA AGATATTATT TAAAAAATAA CATGGAAACA 
GAAACCCTAT GTTCAGATGA AGATGCTCAA GAACTAGTTA AGGAAAGCCA ACTATCCATG 
CTGCAGCTCA GTACCATTGA GGTGGCCACT CAGCTGTCAA TGAGGGACTT TGATTTGTTT 
CGTAATATTG AGCCTACTGA ATACATTGAT GACCTTTTTA AGTTAGATTC CAAAACAGGA 
AATACTCACT TGAAAGAGTT TGAGGACATT GTTAACCAGG AGACATTCTG GGTTGCCTCA 
GAGATTTTAA CGGAATCAAA TCAGTTGAAA CGAATGAAGA TTATTAAGCA TTTTATTAAA 
ATTGCACTTC ATTGTCGAGA ATGTAAGAAC TTCAATTCCA TGTTTGCAAT AATAAGTGGC 
TTGAACCTGG CATCTGTAGC ACGACTCAGA GGTACTTGGG AAAAATTACC AAGCAAGTAT 
GAGAAACACC TTCAAGATCT ACAGGACCTT TTTGATCCAT CTAGAAACAT GGCAAAATAT 
AGAAATATTC TTAGTAGTCA AAGCATGCAG CCTCCAATTA TTCCACTCTT CCCTGTTGTC 
AAGAAAGATA TGACATTTCT ACATGAAGGA AATGACTCCA AAGTAGATGG TTTAGTGAAT 
TTTGAGAAGC TAAGAATGAT TGCCAAGGAG ATCCGTCAGG TTGTTCGAAT GACTTCTGCT 
AACATGGACC CAGCTATGAT GTTTCGACAG AGGAAGAAGA GGTGGCGGAG TCTGGGGTCA 
CTGAGTCAAG GCAGCACAAA CTCAAACATG CTGGATGTTC AAGGAGGTGC ACACAAGAAA 
AGGGCACGTC GCAGCTCTCT ACTTAATGCC AAGAAGTTAT ACGAGGATGC CCAGATGGCA 
AGGAAAGTGA AGCAGTATCT TTCCAGTCTG GATGTCGACA CAGATGAGGA AAAGTTTCAG 
ATGATGTCTT TGCAGTGGGA GCCTGCCTAT GGTACCTTGA CCAAGAATTT AAGTGAGAAA 
AGATCAGCCA AATCATCAGA AATGTCTCCA GTGCCTATGA GATCGGCAGG CCAAACAGCT 
AAAGCACATT TGCATCAGCC CCACCGAGTA AGCCAGGTGC TTCAAGTGCC AGCTGTTAAT 
TTGCACCCCA TCAGGAAGAA GGGACAAGCC AAAGACCCTG CCACGTTGAG TACAAATTTA 
CCTCAGAAAG TTTTAGGAAC AACTGAAGAA GTAAGTGGTA AGAAACATCC AGAAGACACT 
ATCTCTGTGG CATCATCCTT ACATTCTAGC CCTCCTGCAT CTCCTCAAGG TTCCCCTCGC 
AAAGGTTATA CACTTACACC ATCAGCTAAA TCTGACAACT TGTCTGACTC CAGCCATAGT 
GAAATTTCTT CACGATCCAG CATTGTGAGC AATTGTTCTG TTGACTCCAT GTCTACAGCT 
CTACAGGATG AACGGTGTTC TTCACAGGCT GTGGCGGTCC CTGAATCCAC TGGGCCTTTG 
GAAAAGACAG AACACTCCTC AGGGATAGGA GACCATAGTC AGCTTGGCCC CGGGTGGACG 
CTGTCAAAGC CATCTCTAGT CAAGAGCTTA GCTGGCTCAT TTTCTGTGAG TAATGAGGAG 
ATTTCTCATG AGCATGTTAT TTTAGAAGCA GCGGATAGCG GTCGTGGAAG TTGGACTTCT 
TGTTCAAGCA GCTCCCATGA CAACTTCCAA AGCCTTCCAA ACCCAAAAGG CTGGGATTTT 
TTGAACTCTT ATCGACATAC TCATTTGGAT GGCCCCATTG CTGAAGTGGA ACCCCCCGAC 
TGTGAGCCCT ATTCTTGCCC TAAAGGCTGC TCTAGAACTT GTGGTCAGTG TAAAGGAAAC 
CTGGAGACTA ATCAGATGAG ACAGAGTTGG GCATCCTCTA GTTCTCTGTC TGACACATAC 
GAACCAAACT ATGGGACAGT TAAACGGAGA GTGTTAGAGA GCATCACACC TGAGTCCTGT 
GAAAGCTTGG ATCCCAAGGA TGGCACTGAC CCAGTTTACA AAACTGTTAC TTCAAGTACA 
GAAAAGGGCT TGATTGAAAA TGAACAGGTC TCAGCAGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( XM_003431622.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003431670.1 Canis lupus familiaris Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X2, mRNA.

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CloneID	OCe14233
Clone ID Related Accession (Same CDS sequence)	XM_003431622.4
Accession Version	XM_003431622.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	rap guanine nucleotide exchange factor 6 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003431622.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCAC CCGTGGACCC CGGCGCTAGG CAGGCGCTGA AGAAGAAGCC TCCCGAGCGG 
ACCCCCGAGG ATTTAAATAC TATTTATTCT TATCTTCATG GGATGGAAAT ACTATCAAAT 
CTCAGGGAGC ATCAGCTTAG ATTAATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGT 
AATCAAATTC TTTTTTGTTC AGAAACTGTT GCCAGATGTT GGTATATTCT GCTTTCTGGA 
TCTGTGCTTA TGAAAGACTC CATGGTCTTG CCTCCATGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGATAG TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGGCGA 
AGGTTTCGGA AAATTAATTA TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGACATT 
AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATCACAGA CAACCCTCAT 
CCACAGGTGA CTCATGTGTC TTCTAGTCAG TCTGGTTGTA GCATCGCCAG TGACTCTGGG 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACGGAAAGTG AGGTAGGAGA TGTAGATTTA 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGAAATT 
GATCGAACGG ATCCACTACA GGGGCGAGAT CTTGTTCGAG AATGTCTTGA GAAAGAACCT 
GCAGACAAAA CTGATGATGA CATTGAACAA TTACTGGAGT TTATGCACCA GCTCCCAGCT 
TTTGCAAACA TGACCATGTC TGTAAGAAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTGGAGCAGG CTGGAGCTAT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTT 
ATTTTAAACG GCACTGTAGA AATCACTCAT CCAGATGGAA AAGTTGAAAA TCTCTTTATG 
GGAAATAGTT TTGGAATTAC TCCCACTCTG GACAAACAGT ACATGCATGG AGTTGTTAGG 
ACGAAAGTAG ATGATTGTCA GTTTGTCTGC ATAGCGCAGC AGGATTATTG GAGAATTTTA 
AATCATGTGG AGAAAAACAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT CATGGTACAT 
GAGCATCGTG AACTAGATCG GAGTGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC GACTTATATA 
GAAGATTTCC TGCTAACTTA CAGGACATTT CTTGAAAGTC CTTTGGATGT TGGGATCAAA 
CTATTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACAAGGAT TGTGTTATTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCG ATTTCTGGAG 
GAATTTGAAA AAAATCTGGA AGATACGAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCCAA GTGGAGACAG GTGGTGCTGC AAAAGGCTTC CCGGGAGTCC 
CCTCTGCATT TCAGCCTTAA TGGAGGGAGT GAGAAGGGCT TTGGTATTTT TGTTGAAGGA 
GTAGAACCTA GTAGCAAAGC TGCTGATGCT GGACTGAAAC GTGGTGATCA GATAATGGAA 
GTAAATGGAC AAAACTTTGA AAATATTACA TTTGTGAAAG CCCTTGAAAT TTTAAGGAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTACTTAGT 
AGGACTGAAC AAGAAAAATC TGGTGTTCCT CATATTCCTA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATCCAGGA TGTACCGGGA GATATTGAAC ACACATCACA GGAGAAAGGA 
AATAAGAAAA TAAAAGCAAA CACTGTTTCA GGTGGAAGAA ACAAAATTAG GAAAATTTTG 
GATAAAACAC GGTTTAGTAT CTTACCTCCA AAACTGTTTA GTGATGGAGG CCTGAGCCAG 
TCACAAGATG ACAGCATTGT GGGAACAAGA CACTGCAGGC ACAGTCTGGC TATCATGCCT 
ATTCCTGGAA CACTCTCATC CAGCAGCCCT GATCTCTTGC AGCCTACCAC CAGCATGTTG 
GATTTTTCTA ATCCTTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAA 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AAGAAGTTGT CTGTCATGCT 
GTTCATGAAT TTGGTTTGAC TGGTGCATCC GACACGTATT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTCATAAA ACAAAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT 
AGAATTCAGC TCAATGGAAG ATATTATTTA AAAAATAACA TGGAAACAGA AACCCTATGT 
TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAAC TATCCATGCT GCAGCTCAGT 
ACCATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTGTTTCG TAATATTGAG 
CCTACTGAAT ACATTGATGA CCTTTTTAAG TTAGATTCCA AAACAGGAAA TACTCACTTG 
AAAGAGTTTG AGGACATTGT TAACCAGGAG ACATTCTGGG TTGCCTCAGA GATTTTAACG 
GAATCAAATC AGTTGAAACG AATGAAGATT ATTAAGCATT TTATTAAAAT TGCACTTCAT 
TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA 
TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAATTACCAA GCAAGTATGA GAAACACCTT 
CAAGATCTAC AGGACCTTTT TGATCCATCT AGAAACATGG CAAAATATAG AAATATTCTT 
AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG 
ACATTTCTAC ATGAAGGAAA TGACTCCAAA GTAGATGGTT TAGTGAATTT TGAGAAGCTA 
AGAATGATTG CCAAGGAGAT CCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGACCCA 
GCTATGATGT TTCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCACT GAGTCAAGGC 
AGCACAAACT CAAACATGCT GGATGTTCAA GGAGGTGCAC ACAAGAAAAG GGCACGTCGC 
AGCTCTCTAC TTAATGCCAA GAAGTTATAC GAGGATGCCC AGATGGCAAG GAAAGTGAAG 
CAGTATCTTT CCAGTCTGGA TGTCGACACA GATGAGGAAA AGTTTCAGAT GATGTCTTTG 
CAGTGGGAGC CTGCCTATGG TACCTTGACC AAGAATTTAA GTGAGAAAAG ATCAGCCAAA 
TCATCAGAAA TGTCTCCAGT GCCTATGAGA TCGGCAGGCC AAACAGCTAA AGCACATTTG 
CATCAGCCCC ACCGAGTAAG CCAGGTGCTT CAAGTGCCAG CTGTTAATTT GCACCCCATC 
AGGAAGAAGG GACAAGCCAA AGACCCTGCC ACGTTGAGTA CAAATTTACC TCAGAAAGTT 
TTAGGAACAA CTGAAGAAGT AAGTGGTAAG AAACATCCAG AAGACACTAT CTCTGTGGCA 
TCATCCTTAC ATTCTAGCCC TCCTGCATCT CCTCAAGGTT CCCCTCGCAA AGGTTATACA 
CTTACACCAT CAGCTAAATC TGACAACTTG TCTGACTCCA GCCATAGTGA AATTTCTTCA 
CGATCCAGCA TTGTGAGCAA TTGTTCTGTT GACTCCATGT CTACAGCTCT ACAGGATGAA 
CGGTGTTCTT CACAGGCTGT GGCGGTCCCT GAATCCACTG GGCCTTTGGA AAAGACAGAA 
CACTCCTCAG GGATAGGAGA CCATAGTCAG CTTGGCCCCG GGTGGACGCT GTCAAAGCCA 
TCTCTAGTCA AGAGCTTAGC TGGCTCATTT TCTGTGAGTA ATGAGGAGAT TTCTCATGAG 
CATGTTATTT TAGAAGCAGC GGATAGCGGT CGTGGAAGTT GGACTTCTTG TTCAAGCAGC 
TCCCATGACA ACTTCCAAAG CCTTCCAAAC CCAAAAGGCT GGGATTTTTT GAACTCTTAT 
CGACATACTC ATTTGGATGG CCCCATTGCT GAAGTGGAAC CCCCCGACTG TGAGCCCTAT 
TCTTGCCCTA AAGGCTGCTC TAGAACTTGT GGTCAGTGTA AAGGAAACCT GGAGACTAAT 
CAGATGAGAC AGAGTTGGGC ATCCTCTAGT TCTCTGTCTG ACACATACGA ACCAAACTAT 
GGGACAGTTA AACGGAGAGT GTTAGAGAGC ATCACACCTG AGTCCTGTGA AAGCTTGGAT 
CCCAAGGATG GCACTGACCC AGTTTACAAA ACTGTTACTT CAAGTACAGA AAAGGGCTTG 
ATTGTGTACT GTGTCACTTC ACCCAAGAAG GAGGATAGGT ATAGGGAGCC ACCTCCCACT 
CCTCCAGGAT ACATGGGGAT TTCTTTAGCG GACCTAAAGG AAGGACCCCA CCTACACCTA 
AAACCTCCAG ATTATAGCGT GGCAGTGCAG AGGTCAAAGA TGATGCATGA CAGCCTCTCT 
AGACTGCCAC CCACTTCTCT CGGTAGCAGC CCCGTGGCCT GTGTTCCATC GAGGATTGTA 
ACTCAGCCTC AGAGGCATAT TTTGCAGCCA TCCCATCCTA AACTAGCAGA TGTGACTGAC 
GCAGATAGCG AAGCAGATGA AAATGAACAG GTCTCAGCAG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003431670.1
CDS	199..5043
Translation	MNSPVDPGARQALKKKPPERTPEDLNTIYSYLHGMEILSNLREHQLRLMSARARYERYSGNQILFCSETVARCWYILLSGSVLMKDSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENSKDNEDSILQREIPARQSRRRFRKINYKGERQTITDDVDINSYLSLPADLTKMHITDNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEDEEIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAIILEDGQELDSWYVILNGTVEITHPDGKVENLFMGNSFGITPTLDKQYMHGVVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLESPLDVGIKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFEGDPAMTRFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFSLNGGSEKGFGIFVEGVEPSSKAADAGLKRGDQIMEVNGQNFENITFVKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDIEHTSQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKADQQSCYIIISKDTTAKEVVCHAVHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELVKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLDSKTGNTHLKEFEDIVNQETFWVASEILTESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLASVARLRGTWEKLPSKYEKHLQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRQVVRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDVDTDEEKFQMMSLQWEPAYGTLTKNLSEKRSAKSSEMSPVPMRSAGQTAKAHLHQPHRVSQVLQVPAVNLHPIRKKGQAKDPATLSTNLPQKVLGTTEEVSGKKHPEDTISVASSLHSSPPASPQGSPRKGYTLTPSAKSDNLSDSSHSEISSRSSIVSNCSVDSMSTALQDERCSSQAVAVPESTGPLEKTEHSSGIGDHSQLGPGWTLSKPSLVKSLAGSFSVSNEEISHEHVILEAADSGRGSWTSCSSSSHDNFQSLPNPKGWDFLNSYRHTHLDGPIAEVEPPDCEPYSCPKGCSRTCGQCKGNLETNQMRQSWASSSSLSDTYEPNYGTVKRRVLESITPESCESLDPKDGTDPVYKTVTSSTEKGLIVYCVTSPKKEDRYREPPPTPPGYMGISLADLKEGPHLHLKPPDYSVAVQRSKMMHDSLSRLPPTSLGSSPVACVPSRIVTQPQRHILQPSHPKLADVTDADSEADENEQVSAV

Target ORF information:

RefSeq Version	XM_003431622.4
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003431622.4

ORF Insert Sequence:

ATGAACTCAC CCGTGGACCC CGGCGCTAGG CAGGCGCTGA AGAAGAAGCC TCCCGAGCGG 
ACCCCCGAGG ATTTAAATAC TATTTATTCT TATCTTCATG GGATGGAAAT ACTATCAAAT 
CTCAGGGAGC ATCAGCTTAG ATTAATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGT 
AATCAAATTC TTTTTTGTTC AGAAACTGTT GCCAGATGTT GGTATATTCT GCTTTCTGGA 
TCTGTGCTTA TGAAAGACTC CATGGTCTTG CCTCCATGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGATAG TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGGCGA 
AGGTTTCGGA AAATTAATTA TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGACATT 
AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATCACAGA CAACCCTCAT 
CCACAGGTGA CTCATGTGTC TTCTAGTCAG TCTGGTTGTA GCATCGCCAG TGACTCTGGG 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACGGAAAGTG AGGTAGGAGA TGTAGATTTA 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGAAATT 
GATCGAACGG ATCCACTACA GGGGCGAGAT CTTGTTCGAG AATGTCTTGA GAAAGAACCT 
GCAGACAAAA CTGATGATGA CATTGAACAA TTACTGGAGT TTATGCACCA GCTCCCAGCT 
TTTGCAAACA TGACCATGTC TGTAAGAAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTGGAGCAGG CTGGAGCTAT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTT 
ATTTTAAACG GCACTGTAGA AATCACTCAT CCAGATGGAA AAGTTGAAAA TCTCTTTATG 
GGAAATAGTT TTGGAATTAC TCCCACTCTG GACAAACAGT ACATGCATGG AGTTGTTAGG 
ACGAAAGTAG ATGATTGTCA GTTTGTCTGC ATAGCGCAGC AGGATTATTG GAGAATTTTA 
AATCATGTGG AGAAAAACAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT CATGGTACAT 
GAGCATCGTG AACTAGATCG GAGTGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC GACTTATATA 
GAAGATTTCC TGCTAACTTA CAGGACATTT CTTGAAAGTC CTTTGGATGT TGGGATCAAA 
CTATTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACAAGGAT TGTGTTATTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCG ATTTCTGGAG 
GAATTTGAAA AAAATCTGGA AGATACGAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCCAA GTGGAGACAG GTGGTGCTGC AAAAGGCTTC CCGGGAGTCC 
CCTCTGCATT TCAGCCTTAA TGGAGGGAGT GAGAAGGGCT TTGGTATTTT TGTTGAAGGA 
GTAGAACCTA GTAGCAAAGC TGCTGATGCT GGACTGAAAC GTGGTGATCA GATAATGGAA 
GTAAATGGAC AAAACTTTGA AAATATTACA TTTGTGAAAG CCCTTGAAAT TTTAAGGAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTACTTAGT 
AGGACTGAAC AAGAAAAATC TGGTGTTCCT CATATTCCTA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATCCAGGA TGTACCGGGA GATATTGAAC ACACATCACA GGAGAAAGGA 
AATAAGAAAA TAAAAGCAAA CACTGTTTCA GGTGGAAGAA ACAAAATTAG GAAAATTTTG 
GATAAAACAC GGTTTAGTAT CTTACCTCCA AAACTGTTTA GTGATGGAGG CCTGAGCCAG 
TCACAAGATG ACAGCATTGT GGGAACAAGA CACTGCAGGC ACAGTCTGGC TATCATGCCT 
ATTCCTGGAA CACTCTCATC CAGCAGCCCT GATCTCTTGC AGCCTACCAC CAGCATGTTG 
GATTTTTCTA ATCCTTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAA 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AAGAAGTTGT CTGTCATGCT 
GTTCATGAAT TTGGTTTGAC TGGTGCATCC GACACGTATT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTCATAAA ACAAAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT 
AGAATTCAGC TCAATGGAAG ATATTATTTA AAAAATAACA TGGAAACAGA AACCCTATGT 
TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAAC TATCCATGCT GCAGCTCAGT 
ACCATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTGTTTCG TAATATTGAG 
CCTACTGAAT ACATTGATGA CCTTTTTAAG TTAGATTCCA AAACAGGAAA TACTCACTTG 
AAAGAGTTTG AGGACATTGT TAACCAGGAG ACATTCTGGG TTGCCTCAGA GATTTTAACG 
GAATCAAATC AGTTGAAACG AATGAAGATT ATTAAGCATT TTATTAAAAT TGCACTTCAT 
TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA 
TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAATTACCAA GCAAGTATGA GAAACACCTT 
CAAGATCTAC AGGACCTTTT TGATCCATCT AGAAACATGG CAAAATATAG AAATATTCTT 
AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG 
ACATTTCTAC ATGAAGGAAA TGACTCCAAA GTAGATGGTT TAGTGAATTT TGAGAAGCTA 
AGAATGATTG CCAAGGAGAT CCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGACCCA 
GCTATGATGT TTCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCACT GAGTCAAGGC 
AGCACAAACT CAAACATGCT GGATGTTCAA GGAGGTGCAC ACAAGAAAAG GGCACGTCGC 
AGCTCTCTAC TTAATGCCAA GAAGTTATAC GAGGATGCCC AGATGGCAAG GAAAGTGAAG 
CAGTATCTTT CCAGTCTGGA TGTCGACACA GATGAGGAAA AGTTTCAGAT GATGTCTTTG 
CAGTGGGAGC CTGCCTATGG TACCTTGACC AAGAATTTAA GTGAGAAAAG ATCAGCCAAA 
TCATCAGAAA TGTCTCCAGT GCCTATGAGA TCGGCAGGCC AAACAGCTAA AGCACATTTG 
CATCAGCCCC ACCGAGTAAG CCAGGTGCTT CAAGTGCCAG CTGTTAATTT GCACCCCATC 
AGGAAGAAGG GACAAGCCAA AGACCCTGCC ACGTTGAGTA CAAATTTACC TCAGAAAGTT 
TTAGGAACAA CTGAAGAAGT AAGTGGTAAG AAACATCCAG AAGACACTAT CTCTGTGGCA 
TCATCCTTAC ATTCTAGCCC TCCTGCATCT CCTCAAGGTT CCCCTCGCAA AGGTTATACA 
CTTACACCAT CAGCTAAATC TGACAACTTG TCTGACTCCA GCCATAGTGA AATTTCTTCA 
CGATCCAGCA TTGTGAGCAA TTGTTCTGTT GACTCCATGT CTACAGCTCT ACAGGATGAA 
CGGTGTTCTT CACAGGCTGT GGCGGTCCCT GAATCCACTG GGCCTTTGGA AAAGACAGAA 
CACTCCTCAG GGATAGGAGA CCATAGTCAG CTTGGCCCCG GGTGGACGCT GTCAAAGCCA 
TCTCTAGTCA AGAGCTTAGC TGGCTCATTT TCTGTGAGTA ATGAGGAGAT TTCTCATGAG 
CATGTTATTT TAGAAGCAGC GGATAGCGGT CGTGGAAGTT GGACTTCTTG TTCAAGCAGC 
TCCCATGACA ACTTCCAAAG CCTTCCAAAC CCAAAAGGCT GGGATTTTTT GAACTCTTAT 
CGACATACTC ATTTGGATGG CCCCATTGCT GAAGTGGAAC CCCCCGACTG TGAGCCCTAT 
TCTTGCCCTA AAGGCTGCTC TAGAACTTGT GGTCAGTGTA AAGGAAACCT GGAGACTAAT 
CAGATGAGAC AGAGTTGGGC ATCCTCTAGT TCTCTGTCTG ACACATACGA ACCAAACTAT 
GGGACAGTTA AACGGAGAGT GTTAGAGAGC ATCACACCTG AGTCCTGTGA AAGCTTGGAT 
CCCAAGGATG GCACTGACCC AGTTTACAAA ACTGTTACTT CAAGTACAGA AAAGGGCTTG 
ATTGTGTACT GTGTCACTTC ACCCAAGAAG GAGGATAGGT ATAGGGAGCC ACCTCCCACT 
CCTCCAGGAT ACATGGGGAT TTCTTTAGCG GACCTAAAGG AAGGACCCCA CCTACACCTA 
AAACCTCCAG ATTATAGCGT GGCAGTGCAG AGGTCAAAGA TGATGCATGA CAGCCTCTCT 
AGACTGCCAC CCACTTCTCT CGGTAGCAGC CCCGTGGCCT GTGTTCCATC GAGGATTGTA 
ACTCAGCCTC AGAGGCATAT TTTGCAGCCA TCCCATCCTA AACTAGCAGA TGTGACTGAC 
GCAGATAGCG AAGCAGATGA AAATGAACAG GTCTCAGCAG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( XM_846793.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_851886.2 Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 6 (RAPGEF6), transcript variant X4, mRNA.

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CloneID	OCe14234
Clone ID Related Accession (Same CDS sequence)	XM_846793.4
Accession Version	XM_846793.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	rap guanine nucleotide exchange factor 6 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_846793.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCAC CCGTGGACCC CGGCGCTAGG CAGGCGCTGA AGAAGAAGCC TCCCGAGCGG 
ACCCCCGAGG ATTTAAATAC TATTTATTCT TATCTTCATG GGATGGAAAT ACTATCAAAT 
CTCAGGGAGC ATCAGCTTAG ATTAATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGT 
AATCAAATTC TTTTTTGTTC AGAAACTGTT GCCAGATGTT GGTATATTCT GCTTTCTGGA 
TCTGTGCTTA TGAAAGACTC CATGGTCTTG CCTCCATGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGATAG TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGGCGA 
AGGTTTCGGA AAATTAATTA TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGACATT 
AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATCACAGA CAACCCTCAT 
CCACAGGTGA CTCATGTGTC TTCTAGTCAG TCTGGTTGTA GCATCGCCAG TGACTCTGGG 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACGGAAAGTG AGGTAGGAGA TGTAGATTTA 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGAAATT 
GATCGAACGG ATCCACTACA GGGGCGAGAT CTTGTTCGAG AATGTCTTGA GAAAGAACCT 
GCAGACAAAA CTGATGATGA CATTGAACAA TTACTGGAGT TTATGCACCA GCTCCCAGCT 
TTTGCAAACA TGACCATGTC TGTAAGAAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTGGAGCAGG CTGGAGCTAT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTT 
ATTTTAAACG GCACTGTAGA AATCACTCAT CCAGATGGAA AAGTTGAAAA TCTCTTTATG 
GGAAATAGTT TTGGAATTAC TCCCACTCTG GACAAACAGT ACATGCATGG AGTTGTTAGG 
ACGAAAGTAG ATGATTGTCA GTTTGTCTGC ATAGCGCAGC AGGATTATTG GAGAATTTTA 
AATCATGTGG AGAAAAACAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT CATGGTACAT 
GAGCATCGTG AACTAGATCG GAGTGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC GACTTATATA 
GAAGATTTCC TGCTAACTTA CAGGACATTT CTTGAAAGTC CTTTGGATGT TGGGATCAAA 
CTATTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACAAGGAT TGTGTTATTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCG ATTTCTGGAG 
GAATTTGAAA AAAATCTGGA AGATACGAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCCAA GTGGAGACAG GTGGTGCTGC AAAAGGCTTC CCGGGAGTCC 
CCTCTGCATT TCAGCCTTAA TGGAGGGAGT GAGAAGGGCT TTGGTATTTT TGTTGAAGGA 
GTAGAACCTA GTAGCAAAGC TGCTGATGCT GGACTGAAAC GTGGTGATCA GATAATGGAA 
GTAAATGGAC AAAACTTTGA AAATATTACA TTTGTGAAAG CCCTTGAAAT TTTAAGGAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTACTTAGT 
AGGACTGAAC AAGAAAAATC TGGTGTTCCT CATATTCCTA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATCCAGGA TGTACCGGGA GATATTGAAC ACACATCACA GGAGAAAGGA 
AATAAGAAAA TAAAAGCAAA CACTGTTTCA GGTGGAAGAA ACAAAATTAG GAAAATTTTG 
GATAAAACAC GGTTTAGTAT CTTACCTCCA AAACTGTTTA GTGATGGAGG CCTGAGCCAG 
TCACAAGATG ACAGCATTGT GGGAACAAGA CACTGCAGGC ACAGTCTGGC TATCATGCCT 
ATTCCTGGAA CACTCTCATC CAGCAGCCCT GATCTCTTGC AGCCTACCAC CAGCATGTTG 
GATTTTTCTA ATCCTTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAA 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AAGAAGTTGT CTGTCATGCT 
GTTCATGAAT TTGGTTTGAC TGGTGCATCC GACACGTATT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTCATAAA ACAAAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT 
AGAATTCAGC TCAATGGAAG ATATTATTTA AAAAATAACA TGGAAACAGA AACCCTATGT 
TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAAC TATCCATGCT GCAGCTCAGT 
ACCATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTGTTTCG TAATATTGAG 
CCTACTGAAT ACATTGATGA CCTTTTTAAG TTAGATTCCA AAACAGGAAA TACTCACTTG 
AAAGAGTTTG AGGACATTGT TAACCAGGAG ACATTCTGGG TTGCCTCAGA GATTTTAACG 
GAATCAAATC AGTTGAAACG AATGAAGATT ATTAAGCATT TTATTAAAAT TGCACTTCAT 
TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA 
TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAATTACCAA GCAAGTATGA GAAACACCTT 
CAAGATCTAC AGGACCTTTT TGATCCATCT AGAAACATGG CAAAATATAG AAATATTCTT 
AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG 
ACATTTCTAC ATGAAGGAAA TGACTCCAAA GTAGATGGTT TAGTGAATTT TGAGAAGCTA 
AGAATGATTG CCAAGGAGAT CCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGACCCA 
GCTATGATGT TTCGACAGAG GTCACTGAGT CAAGGCAGCA CAAACTCAAA CATGCTGGAT 
GTTCAAGGAG GTGCACACAA GAAAAGGGCA CGTCGCAGCT CTCTACTTAA TGCCAAGAAG 
TTATACGAGG ATGCCCAGAT GGCAAGGAAA GTGAAGCAGT ATCTTTCCAG TCTGGATGTC 
GACACAGATG AGGAAAAGTT TCAGATGATG TCTTTGCAGT GGGAGCCTGC CTATGGTACC 
TTGACCAAGA ATTTAAGTGA GAAAAGATCA GCCAAATCAT CAGAAATGTC TCCAGTGCCT 
ATGAGATCGG CAGGCCAAAC AGCTAAAGCA CATTTGCATC AGCCCCACCG AGTAAGCCAG 
GTGCTTCAAG TGCCAGCTGT TAATTTGCAC CCCATCAGGA AGAAGGGACA AGCCAAAGAC 
CCTGCCACGT TGAGTACAAA TTTACCTCAG AAAGTTTTAG GAACAACTGA AGAAGTAAGT 
GGTAAGAAAC ATCCAGAAGA CACTATCTCT GTGGCATCAT CCTTACATTC TAGCCCTCCT 
GCATCTCCTC AAGGTTCCCC TCGCAAAGGT TATACACTTA CACCATCAGC TAAATCTGAC 
AACTTGTCTG ACTCCAGCCA TAGTGAAATT TCTTCACGAT CCAGCATTGT GAGCAATTGT 
TCTGTTGACT CCATGTCTAC AGCTCTACAG GATGAACGGT GTTCTTCACA GGCTGTGGCG 
GTCCCTGAAT CCACTGGGCC TTTGGAAAAG ACAGAACACT CCTCAGGGAT AGGAGACCAT 
AGTCAGCTTG GCCCCGGGTG GACGCTGTCA AAGCCATCTC TAGTCAAGAG CTTAGCTGGC 
TCATTTTCTG TGAGTAATGA GGAGATTTCT CATGAGCATG TTATTTTAGA AGCAGCGGAT 
AGCGGTCGTG GAAGTTGGAC TTCTTGTTCA AGCAGCTCCC ATGACAACTT CCAAAGCCTT 
CCAAACCCAA AAGGCTGGGA TTTTTTGAAC TCTTATCGAC ATACTCATTT GGATGGCCCC 
ATTGCTGAAG TGGAACCCCC CGACTGTGAG CCCTATTCTT GCCCTAAAGG CTGCTCTAGA 
ACTTGTGGTC AGTGTAAAGG AAACCTGGAG ACTAATCAGA TGAGACAGAG TTGGGCATCC 
TCTAGTTCTC TGTCTGACAC ATACGAACCA AACTATGGGA CAGTTAAACG GAGAGTGTTA 
GAGAGCATCA CACCTGAGTC CTGTGAAAGC TTGGATCCCA AGGATGGCAC TGACCCAGTT 
TACAAAACTG TTACTTCAAG TACAGAAAAG GGCTTGATTG TGTACTGTGT CACTTCACCC 
AAGAAGGAGG ATAGGTATAG GGAGCCACCT CCCACTCCTC CAGGATACAT GGGGATTTCT 
TTAGCGGACC TAAAGGAAGG ACCCCACCTA CACCTAAAAC CTCCAGATTA TAGCGTGGCA 
GTGCAGAGGT CAAAGATGAT GCATGACAGC CTCTCTAGAC TGCCACCCAC TTCTCTCGGT 
AGCAGCCCCG TGGCCTGTGT TCCATCGAGG ATTGTAACTC AGCCTCAGAG GCATATTTTG 
CAGCCATCCC ATCCTAAACT AGCAGATGTG ACTGACGCAG ATAGCGAAGC AGATGAAAAT 
GAACAGGTCT CAGCAGTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_851886.2
CDS	238..5058
Translation	MNSPVDPGARQALKKKPPERTPEDLNTIYSYLHGMEILSNLREHQLRLMSARARYERYSGNQILFCSETVARCWYILLSGSVLMKDSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENSKDNEDSILQREIPARQSRRRFRKINYKGERQTITDDVDINSYLSLPADLTKMHITDNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEDEEIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAIILEDGQELDSWYVILNGTVEITHPDGKVENLFMGNSFGITPTLDKQYMHGVVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLESPLDVGIKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFEGDPAMTRFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFSLNGGSEKGFGIFVEGVEPSSKAADAGLKRGDQIMEVNGQNFENITFVKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDIEHTSQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKADQQSCYIIISKDTTAKEVVCHAVHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELVKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLDSKTGNTHLKEFEDIVNQETFWVASEILTESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLASVARLRGTWEKLPSKYEKHLQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRQVVRMTSANMDPAMMFRQRSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDVDTDEEKFQMMSLQWEPAYGTLTKNLSEKRSAKSSEMSPVPMRSAGQTAKAHLHQPHRVSQVLQVPAVNLHPIRKKGQAKDPATLSTNLPQKVLGTTEEVSGKKHPEDTISVASSLHSSPPASPQGSPRKGYTLTPSAKSDNLSDSSHSEISSRSSIVSNCSVDSMSTALQDERCSSQAVAVPESTGPLEKTEHSSGIGDHSQLGPGWTLSKPSLVKSLAGSFSVSNEEISHEHVILEAADSGRGSWTSCSSSSHDNFQSLPNPKGWDFLNSYRHTHLDGPIAEVEPPDCEPYSCPKGCSRTCGQCKGNLETNQMRQSWASSSSLSDTYEPNYGTVKRRVLESITPESCESLDPKDGTDPVYKTVTSSTEKGLIVYCVTSPKKEDRYREPPPTPPGYMGISLADLKEGPHLHLKPPDYSVAVQRSKMMHDSLSRLPPTSLGSSPVACVPSRIVTQPQRHILQPSHPKLADVTDADSEADENEQVSAV

Target ORF information:

RefSeq Version	XM_846793.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris Rap guanine nucleotide exchange factor (GEF) 6 (RAPGEF6), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_846793.4

ORF Insert Sequence:

ATGAACTCAC CCGTGGACCC CGGCGCTAGG CAGGCGCTGA AGAAGAAGCC TCCCGAGCGG 
ACCCCCGAGG ATTTAAATAC TATTTATTCT TATCTTCATG GGATGGAAAT ACTATCAAAT 
CTCAGGGAGC ATCAGCTTAG ATTAATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGT 
AATCAAATTC TTTTTTGTTC AGAAACTGTT GCCAGATGTT GGTATATTCT GCTTTCTGGA 
TCTGTGCTTA TGAAAGACTC CATGGTCTTG CCTCCATGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGATAG TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGGCGA 
AGGTTTCGGA AAATTAATTA TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGACATT 
AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATCACAGA CAACCCTCAT 
CCACAGGTGA CTCATGTGTC TTCTAGTCAG TCTGGTTGTA GCATCGCCAG TGACTCTGGG 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACGGAAAGTG AGGTAGGAGA TGTAGATTTA 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGAAATT 
GATCGAACGG ATCCACTACA GGGGCGAGAT CTTGTTCGAG AATGTCTTGA GAAAGAACCT 
GCAGACAAAA CTGATGATGA CATTGAACAA TTACTGGAGT TTATGCACCA GCTCCCAGCT 
TTTGCAAACA TGACCATGTC TGTAAGAAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTGGAGCAGG CTGGAGCTAT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTT 
ATTTTAAACG GCACTGTAGA AATCACTCAT CCAGATGGAA AAGTTGAAAA TCTCTTTATG 
GGAAATAGTT TTGGAATTAC TCCCACTCTG GACAAACAGT ACATGCATGG AGTTGTTAGG 
ACGAAAGTAG ATGATTGTCA GTTTGTCTGC ATAGCGCAGC AGGATTATTG GAGAATTTTA 
AATCATGTGG AGAAAAACAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT CATGGTACAT 
GAGCATCGTG AACTAGATCG GAGTGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC GACTTATATA 
GAAGATTTCC TGCTAACTTA CAGGACATTT CTTGAAAGTC CTTTGGATGT TGGGATCAAA 
CTATTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACAAGGAT TGTGTTATTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCG ATTTCTGGAG 
GAATTTGAAA AAAATCTGGA AGATACGAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCCAA GTGGAGACAG GTGGTGCTGC AAAAGGCTTC CCGGGAGTCC 
CCTCTGCATT TCAGCCTTAA TGGAGGGAGT GAGAAGGGCT TTGGTATTTT TGTTGAAGGA 
GTAGAACCTA GTAGCAAAGC TGCTGATGCT GGACTGAAAC GTGGTGATCA GATAATGGAA 
GTAAATGGAC AAAACTTTGA AAATATTACA TTTGTGAAAG CCCTTGAAAT TTTAAGGAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTACTTAGT 
AGGACTGAAC AAGAAAAATC TGGTGTTCCT CATATTCCTA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATCCAGGA TGTACCGGGA GATATTGAAC ACACATCACA GGAGAAAGGA 
AATAAGAAAA TAAAAGCAAA CACTGTTTCA GGTGGAAGAA ACAAAATTAG GAAAATTTTG 
GATAAAACAC GGTTTAGTAT CTTACCTCCA AAACTGTTTA GTGATGGAGG CCTGAGCCAG 
TCACAAGATG ACAGCATTGT GGGAACAAGA CACTGCAGGC ACAGTCTGGC TATCATGCCT 
ATTCCTGGAA CACTCTCATC CAGCAGCCCT GATCTCTTGC AGCCTACCAC CAGCATGTTG 
GATTTTTCTA ATCCTTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAA 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AAGAAGTTGT CTGTCATGCT 
GTTCATGAAT TTGGTTTGAC TGGTGCATCC GACACGTATT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTCATAAA ACAAAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT 
AGAATTCAGC TCAATGGAAG ATATTATTTA AAAAATAACA TGGAAACAGA AACCCTATGT 
TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAAC TATCCATGCT GCAGCTCAGT 
ACCATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTGTTTCG TAATATTGAG 
CCTACTGAAT ACATTGATGA CCTTTTTAAG TTAGATTCCA AAACAGGAAA TACTCACTTG 
AAAGAGTTTG AGGACATTGT TAACCAGGAG ACATTCTGGG TTGCCTCAGA GATTTTAACG 
GAATCAAATC AGTTGAAACG AATGAAGATT ATTAAGCATT TTATTAAAAT TGCACTTCAT 
TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA 
TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAATTACCAA GCAAGTATGA GAAACACCTT 
CAAGATCTAC AGGACCTTTT TGATCCATCT AGAAACATGG CAAAATATAG AAATATTCTT 
AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG 
ACATTTCTAC ATGAAGGAAA TGACTCCAAA GTAGATGGTT TAGTGAATTT TGAGAAGCTA 
AGAATGATTG CCAAGGAGAT CCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGACCCA 
GCTATGATGT TTCGACAGAG GTCACTGAGT CAAGGCAGCA CAAACTCAAA CATGCTGGAT 
GTTCAAGGAG GTGCACACAA GAAAAGGGCA CGTCGCAGCT CTCTACTTAA TGCCAAGAAG 
TTATACGAGG ATGCCCAGAT GGCAAGGAAA GTGAAGCAGT ATCTTTCCAG TCTGGATGTC 
GACACAGATG AGGAAAAGTT TCAGATGATG TCTTTGCAGT GGGAGCCTGC CTATGGTACC 
TTGACCAAGA ATTTAAGTGA GAAAAGATCA GCCAAATCAT CAGAAATGTC TCCAGTGCCT 
ATGAGATCGG CAGGCCAAAC AGCTAAAGCA CATTTGCATC AGCCCCACCG AGTAAGCCAG 
GTGCTTCAAG TGCCAGCTGT TAATTTGCAC CCCATCAGGA AGAAGGGACA AGCCAAAGAC 
CCTGCCACGT TGAGTACAAA TTTACCTCAG AAAGTTTTAG GAACAACTGA AGAAGTAAGT 
GGTAAGAAAC ATCCAGAAGA CACTATCTCT GTGGCATCAT CCTTACATTC TAGCCCTCCT 
GCATCTCCTC AAGGTTCCCC TCGCAAAGGT TATACACTTA CACCATCAGC TAAATCTGAC 
AACTTGTCTG ACTCCAGCCA TAGTGAAATT TCTTCACGAT CCAGCATTGT GAGCAATTGT 
TCTGTTGACT CCATGTCTAC AGCTCTACAG GATGAACGGT GTTCTTCACA GGCTGTGGCG 
GTCCCTGAAT CCACTGGGCC TTTGGAAAAG ACAGAACACT CCTCAGGGAT AGGAGACCAT 
AGTCAGCTTG GCCCCGGGTG GACGCTGTCA AAGCCATCTC TAGTCAAGAG CTTAGCTGGC 
TCATTTTCTG TGAGTAATGA GGAGATTTCT CATGAGCATG TTATTTTAGA AGCAGCGGAT 
AGCGGTCGTG GAAGTTGGAC TTCTTGTTCA AGCAGCTCCC ATGACAACTT CCAAAGCCTT 
CCAAACCCAA AAGGCTGGGA TTTTTTGAAC TCTTATCGAC ATACTCATTT GGATGGCCCC 
ATTGCTGAAG TGGAACCCCC CGACTGTGAG CCCTATTCTT GCCCTAAAGG CTGCTCTAGA 
ACTTGTGGTC AGTGTAAAGG AAACCTGGAG ACTAATCAGA TGAGACAGAG TTGGGCATCC 
TCTAGTTCTC TGTCTGACAC ATACGAACCA AACTATGGGA CAGTTAAACG GAGAGTGTTA 
GAGAGCATCA CACCTGAGTC CTGTGAAAGC TTGGATCCCA AGGATGGCAC TGACCCAGTT 
TACAAAACTG TTACTTCAAG TACAGAAAAG GGCTTGATTG TGTACTGTGT CACTTCACCC 
AAGAAGGAGG ATAGGTATAG GGAGCCACCT CCCACTCCTC CAGGATACAT GGGGATTTCT 
TTAGCGGACC TAAAGGAAGG ACCCCACCTA CACCTAAAAC CTCCAGATTA TAGCGTGGCA 
GTGCAGAGGT CAAAGATGAT GCATGACAGC CTCTCTAGAC TGCCACCCAC TTCTCTCGGT 
AGCAGCCCCG TGGCCTGTGT TCCATCGAGG ATTGTAACTC AGCCTCAGAG GCATATTTTG 
CAGCCATCCC ATCCTAAACT AGCAGATGTG ACTGACGCAG ATAGCGAAGC AGATGAAAAT 
GAACAGGTCT CAGCAGTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( XM_005626500.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005626557.1 Canis lupus familiaris Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X11, mRNA.

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CloneID	OCe14241
Clone ID Related Accession (Same CDS sequence)	XM_005626500.3
Accession Version	XM_005626500.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4230bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	rap guanine nucleotide exchange factor 6 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005626500.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCAC CCGTGGACCC CGGCGCTAGG CAGGCGCTGA AGAAGAAGCC TCCCGAGCGG 
ACCCCCGAGG ATTTAAATAC TATTTATTCT TATCTTCATG GGATGGAAAT ACTATCAAAT 
CTCAGGGAGC ATCAGCTTAG ATTAATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGT 
AATCAAATTC TTTTTTGTTC AGAAACTGTT GCCAGATGTT GGTATATTCT GCTTTCTGGA 
TCTGTGCTTA TGAAAGACTC CATGGTCTTG CCTCCATGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGATAG TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGGCGA 
AGGTTTCGGA AAATTAATTA TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGACATT 
AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATCACAGA CAACCCTCAT 
CCACAGGTGA CTCATGTGTC TTCTAGTCAG TCTGGTTGTA GCATCGCCAG TGACTCTGGG 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACGGAAAGTG AGGTAGGAGA TGTAGATTTA 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGAAATT 
GATCGAACGG ATCCACTACA GGGGCGAGAT CTTGTTCGAG AATGTCTTGA GAAAGAACCT 
GCAGACAAAA CTGATGATGA CATTGAACAA TTACTGGAGT TTATGCACCA GCTCCCAGCT 
TTTGCAAACA TGACCATGTC TGTAAGAAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTGGAGCAGG CTGGAGCTAT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTT 
ATTTTAAACG GCACTGTAGA AATCACTCAT CCAGATGGAA AAGTTGAAAA TCTCTTTATG 
GGAAATAGTT TTGGAATTAC TCCCACTCTG GACAAACAGT ACATGCATGG AGTTGTTAGG 
ACGAAAGTAG ATGATTGTCA GTTTGTCTGC ATAGCGCAGC AGGATTATTG GAGAATTTTA 
AATCATGTGG AGAAAAACAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT CATGGTACAT 
GAGCATCGTG AACTAGATCG GAGTGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC GACTTATATA 
GAAGATTTCC TGCTAACTTA CAGGACATTT CTTGAAAGTC CTTTGGATGT TGGGATCAAA 
CTATTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACAAGGAT TGTGTTATTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCG ATTTCTGGAG 
GAATTTGAAA AAAATCTGGA AGATACGAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCCAA GTGGAGACAG GTGGTGCTGC AAAAGGCTTC CCGGGAGTCC 
CCTCTGCATT TCAGCCTTAA TGGAGGGAGT GAGAAGGGCT TTGGTATTTT TGTTGAAGGA 
GTAGAACCTA GTAGCAAAGC TGCTGATGCT GGACTGAAAC GTGGTGATCA GATAATGGAA 
GTAAATGGAC AAAACTTTGA AAATATTACA TTTGTGAAAG CCCTTGAAAT TTTAAGGAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTACTTAGT 
AGGACTGAAC AAGAAAAATC TGGTGTTCCT CATATTCCTA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATCCAGGA TGTACCGGGA GATATTGAAC ACACATCACA GGAGAAAGGA 
AATAAGAAAA TAAAAGCAAA CACTGTTTCA GGTGGAAGAA ACAAAATTAG GAAAATTTTG 
GATAAAACAC GGTTTAGTAT CTTACCTCCA AAACTGTTTA GTGATGGAGG CCTGAGCCAG 
TCACAAGATG ACAGCATTGT GGGAACAAGA CACTGCAGGC ACAGTCTGGC TATCATGCCT 
ATTCCTGGAA CACTCTCATC CAGCAGCCCT GATCTCTTGC AGCCTACCAC CAGCATGTTG 
GATTTTTCTA ATCCTTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAA 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AAGAAGTTGT CTGTCATGCT 
GTTCATGAAT TTGGTTTGAC TGGTGCATCC GACACGTATT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTCATAAA ACAAAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT 
AGAATTCAGC TCAATGGAAG ATATTATTTA AAAAATAACA TGGAAACAGA AACCCTATGT 
TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAAC TATCCATGCT GCAGCTCAGT 
ACCATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTGTTTCG TAATATTGAG 
CCTACTGAAT ACATTGATGA CCTTTTTAAG TTAGATTCCA AAACAGGAAA TACTCACTTG 
AAAGAGTTTG AGGACATTGT TAACCAGGAG ACATTCTGGG TTGCCTCAGA GATTTTAACG 
GAATCAAATC AGTTGAAACG AATGAAGATT ATTAAGCATT TTATTAAAAT TGCACTTCAT 
TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA 
TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAATTACCAA GCAAGTATGA GAAACACCTT 
CAAGATCTAC AGGACCTTTT TGATCCATCT AGAAACATGG CAAAATATAG AAATATTCTT 
AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG 
ACATTTCTAC ATGAAGGAAA TGACTCCAAA GTAGATGGTT TAGTGAATTT TGAGAAGCTA 
AGAATGATTG CCAAGGAGAT CCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGACCCA 
GCTATGATGT TTCGACAGAG GTCACTGAGT CAAGGCAGCA CAAACTCAAA CATGCTGGAT 
GTTCAAGGAG GTGCACACAA GAAAAGGGCA CGTCGCAGCT CTCTACTTAA TGCCAAGAAG 
TTATACGAGG ATGCCCAGAT GGCAAGGAAA GTGAAGCAGT ATCTTTCCAG TCTGGATGTC 
GACACAGATG AGGAAAAGTT TCAGATGATG TCTTTGCAGT GGGAGCCTGC CTATGGTACC 
TTGACCAAGA ATTTAAGTGA GAAAAGATCA GCCAAATCAT CAGAAATGTC TCCAGTGCCT 
ATGAGATCGG CAGGCCAAAC AGCTAAAGCA CATTTGCATC AGCCCCACCG AGTAAGCCAG 
GTGCTTCAAG TGCCAGCTGT TAATTTGCAC CCCATCAGGA AGAAGGGACA AGCCAAAGAC 
CCTGCCACGT TGAGTACAAA TTTACCTCAG AAAGTTTTAG GAACAACTGA AGAAGTAAGT 
GGTAAGAAAC ATCCAGAAGA CACTATCTCT GTGGCATCAT CCTTACATTC TAGCCCTCCT 
GCATCTCCTC AAGGTTCCCC TCGCAAAGTT GGCAGTATCC TATCTGATCT CAGCATCCTG 
CTGCGCCTCT CAGGGTCTTC CTCATCCCCT CCCCGTGAGT CCAGCACCAA GATCAGTGGC 
CAGAGCTGCC CCGGAATAGG TGGGGTTTAC TTGCAAAAGA AAATCCTTCA GATTACCAGG 
AGCACAGCCA AAAGAACAGA CAGGACCGAG AAGGCCACTG AGGAGAATAG AGACAGGACA 
AGTTGTGAGA ACACAGCCAG AAAAAGAATG ACGTCCCCGT TTAGAAGATT TAGGGAAAGA 
ACTCTTTCAA GGGAGAGACT AGCCAACAGC AAAAAAGAGG ACGCAGATCA CCATCAAGCT 
ACAGAAAGCT GTGAGAAAGT GAAGGATGTT GGGAGCAACA TCAAAGATGA GAAAGGGAGT 
GACATCTTCA ATTCAAATAG CCGAGGCAAC CGTAACACTT TAAACTGCTT CTATACGCGA 
TTCAAGTCTA AGAGGAGAAA GACAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005626557.1
CDS	1..4230
Translation	MNSPVDPGARQALKKKPPERTPEDLNTIYSYLHGMEILSNLREHQLRLMSARARYERYSGNQILFCSETVARCWYILLSGSVLMKDSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENSKDNEDSILQREIPARQSRRRFRKINYKGERQTITDDVDINSYLSLPADLTKMHITDNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEDEEIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAIILEDGQELDSWYVILNGTVEITHPDGKVENLFMGNSFGITPTLDKQYMHGVVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLESPLDVGIKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFEGDPAMTRFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFSLNGGSEKGFGIFVEGVEPSSKAADAGLKRGDQIMEVNGQNFENITFVKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDIEHTSQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKADQQSCYIIISKDTTAKEVVCHAVHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELVKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLDSKTGNTHLKEFEDIVNQETFWVASEILTESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLASVARLRGTWEKLPSKYEKHLQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRQVVRMTSANMDPAMMFRQRSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDVDTDEEKFQMMSLQWEPAYGTLTKNLSEKRSAKSSEMSPVPMRSAGQTAKAHLHQPHRVSQVLQVPAVNLHPIRKKGQAKDPATLSTNLPQKVLGTTEEVSGKKHPEDTISVASSLHSSPPASPQGSPRKVGSILSDLSILLRLSGSSSSPPRESSTKISGQSCPGIGGVYLQKKILQITRSTAKRTDRTEKATEENRDRTSCENTARKRMTSPFRRFRERTLSRERLANSKKEDADHHQATESCEKVKDVGSNIKDEKGSDIFNSNSRGNRNTLNCFYTRFKSKRRKTI

Target ORF information:

RefSeq Version	XM_005626500.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005626500.3

ORF Insert Sequence:

ATGAACTCAC CCGTGGACCC CGGCGCTAGG CAGGCGCTGA AGAAGAAGCC TCCCGAGCGG 
ACCCCCGAGG ATTTAAATAC TATTTATTCT TATCTTCATG GGATGGAAAT ACTATCAAAT 
CTCAGGGAGC ATCAGCTTAG ATTAATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGT 
AATCAAATTC TTTTTTGTTC AGAAACTGTT GCCAGATGTT GGTATATTCT GCTTTCTGGA 
TCTGTGCTTA TGAAAGACTC CATGGTCTTG CCTCCATGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGATAG TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGGCGA 
AGGTTTCGGA AAATTAATTA TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGACATT 
AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATCACAGA CAACCCTCAT 
CCACAGGTGA CTCATGTGTC TTCTAGTCAG TCTGGTTGTA GCATCGCCAG TGACTCTGGG 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACGGAAAGTG AGGTAGGAGA TGTAGATTTA 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGAAATT 
GATCGAACGG ATCCACTACA GGGGCGAGAT CTTGTTCGAG AATGTCTTGA GAAAGAACCT 
GCAGACAAAA CTGATGATGA CATTGAACAA TTACTGGAGT TTATGCACCA GCTCCCAGCT 
TTTGCAAACA TGACCATGTC TGTAAGAAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTGGAGCAGG CTGGAGCTAT TATTCTTGAA GATGGGCAAG AGCTTGACTC ATGGTATGTT 
ATTTTAAACG GCACTGTAGA AATCACTCAT CCAGATGGAA AAGTTGAAAA TCTCTTTATG 
GGAAATAGTT TTGGAATTAC TCCCACTCTG GACAAACAGT ACATGCATGG AGTTGTTAGG 
ACGAAAGTAG ATGATTGTCA GTTTGTCTGC ATAGCGCAGC AGGATTATTG GAGAATTTTA 
AATCATGTGG AGAAAAACAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT CATGGTACAT 
GAGCATCGTG AACTAGATCG GAGTGGAACC AGGAAAGGAC ACATTGTAAT CAAGGCAACA 
CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC GACTTATATA 
GAAGATTTCC TGCTAACTTA CAGGACATTT CTTGAAAGTC CTTTGGATGT TGGGATCAAA 
CTATTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACAAGGAT TGTGTTATTA 
TGGGTAAATA ATCATTTTAA TGATTTTGAA GGTGATCCTG CTATGACTCG ATTTCTGGAG 
GAATTTGAAA AAAATCTGGA AGATACGAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCCTGTGCTG CAAAGGCCAA GTGGAGACAG GTGGTGCTGC AAAAGGCTTC CCGGGAGTCC 
CCTCTGCATT TCAGCCTTAA TGGAGGGAGT GAGAAGGGCT TTGGTATTTT TGTTGAAGGA 
GTAGAACCTA GTAGCAAAGC TGCTGATGCT GGACTGAAAC GTGGTGATCA GATAATGGAA 
GTAAATGGAC AAAACTTTGA AAATATTACA TTTGTGAAAG CCCTTGAAAT TTTAAGGAAT 
AATACTCATC TTGCACTTAC TGTGAAGACC AACATTTTTG TGTTCAAAGA ATTACTTAGT 
AGGACTGAAC AAGAAAAATC TGGTGTTCCT CATATTCCTA AAATTGCTGA AAAAAAAAGT 
AATCGCCATT CAATCCAGGA TGTACCGGGA GATATTGAAC ACACATCACA GGAGAAAGGA 
AATAAGAAAA TAAAAGCAAA CACTGTTTCA GGTGGAAGAA ACAAAATTAG GAAAATTTTG 
GATAAAACAC GGTTTAGTAT CTTACCTCCA AAACTGTTTA GTGATGGAGG CCTGAGCCAG 
TCACAAGATG ACAGCATTGT GGGAACAAGA CACTGCAGGC ACAGTCTGGC TATCATGCCT 
ATTCCTGGAA CACTCTCATC CAGCAGCCCT GATCTCTTGC AGCCTACCAC CAGCATGTTG 
GATTTTTCTA ATCCTTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAA 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AAGAAGTTGT CTGTCATGCT 
GTTCATGAAT TTGGTTTGAC TGGTGCATCC GACACGTATT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTCATAAA ACAAAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT 
AGAATTCAGC TCAATGGAAG ATATTATTTA AAAAATAACA TGGAAACAGA AACCCTATGT 
TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAAC TATCCATGCT GCAGCTCAGT 
ACCATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTGTTTCG TAATATTGAG 
CCTACTGAAT ACATTGATGA CCTTTTTAAG TTAGATTCCA AAACAGGAAA TACTCACTTG 
AAAGAGTTTG AGGACATTGT TAACCAGGAG ACATTCTGGG TTGCCTCAGA GATTTTAACG 
GAATCAAATC AGTTGAAACG AATGAAGATT ATTAAGCATT TTATTAAAAT TGCACTTCAT 
TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGCTT GAACCTGGCA 
TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAATTACCAA GCAAGTATGA GAAACACCTT 
CAAGATCTAC AGGACCTTTT TGATCCATCT AGAAACATGG CAAAATATAG AAATATTCTT 
AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTCC CTGTTGTCAA GAAAGATATG 
ACATTTCTAC ATGAAGGAAA TGACTCCAAA GTAGATGGTT TAGTGAATTT TGAGAAGCTA 
AGAATGATTG CCAAGGAGAT CCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGACCCA 
GCTATGATGT TTCGACAGAG GTCACTGAGT CAAGGCAGCA CAAACTCAAA CATGCTGGAT 
GTTCAAGGAG GTGCACACAA GAAAAGGGCA CGTCGCAGCT CTCTACTTAA TGCCAAGAAG 
TTATACGAGG ATGCCCAGAT GGCAAGGAAA GTGAAGCAGT ATCTTTCCAG TCTGGATGTC 
GACACAGATG AGGAAAAGTT TCAGATGATG TCTTTGCAGT GGGAGCCTGC CTATGGTACC 
TTGACCAAGA ATTTAAGTGA GAAAAGATCA GCCAAATCAT CAGAAATGTC TCCAGTGCCT 
ATGAGATCGG CAGGCCAAAC AGCTAAAGCA CATTTGCATC AGCCCCACCG AGTAAGCCAG 
GTGCTTCAAG TGCCAGCTGT TAATTTGCAC CCCATCAGGA AGAAGGGACA AGCCAAAGAC 
CCTGCCACGT TGAGTACAAA TTTACCTCAG AAAGTTTTAG GAACAACTGA AGAAGTAAGT 
GGTAAGAAAC ATCCAGAAGA CACTATCTCT GTGGCATCAT CCTTACATTC TAGCCCTCCT 
GCATCTCCTC AAGGTTCCCC TCGCAAAGTT GGCAGTATCC TATCTGATCT CAGCATCCTG 
CTGCGCCTCT CAGGGTCTTC CTCATCCCCT CCCCGTGAGT CCAGCACCAA GATCAGTGGC 
CAGAGCTGCC CCGGAATAGG TGGGGTTTAC TTGCAAAAGA AAATCCTTCA GATTACCAGG 
AGCACAGCCA AAAGAACAGA CAGGACCGAG AAGGCCACTG AGGAGAATAG AGACAGGACA 
AGTTGTGAGA ACACAGCCAG AAAAAGAATG ACGTCCCCGT TTAGAAGATT TAGGGAAAGA 
ACTCTTTCAA GGGAGAGACT AGCCAACAGC AAAAAAGAGG ACGCAGATCA CCATCAAGCT 
ACAGAAAGCT GTGAGAAAGT GAAGGATGTT GGGAGCAACA TCAAAGATGA GAAAGGGAGT 
GACATCTTCA ATTCAAATAG CCGAGGCAAC CGTAACACTT TAAACTGCTT CTATACGCGA 
TTCAAGTCTA AGAGGAGAAA GACAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	RAPGEF6
Entrez Gene ID	609521
Full Name	Rap guanine nucleotide exchange factor 6
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

Bos taurus (cattle)	RAPGEF6	XP_003586323.1
Homo sapiens (human)	RAPGEF6	NP_001157858.1
Canis lupus familiaris (dog)	RAPGEF6	XP_003431670.1
Mus musculus (house mouse)	Rapgef6	NP_780467.2
Gallus gallus (chicken)	RAPGEF6	XP_004945062.1
Pan troglodytes (chimpanzee)	RAPGEF6	XP_003339150.1
	RAPGEF6	XP_001101236.2
Rattus norvegicus (Norway rat)	Rapgef6	NP_001100473.1
Danio rerio (zebrafish)	rapgef6	XP_002666140.3
Caenorhabditis elegans (roundworm)	pxf-1	NP_001023389.1

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RAPGEF6 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

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