MYH2 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	MYH2
Entrez Gene ID	608242
Full Name	myosin, heavy chain 2, skeletal muscle, adult
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001076795.1	NP_001070263.1	myosin-2
XM_005619532.2	XP_005619589.1	myosin-2 isoform X1
XM_014113203.1	XP_013968678.1	myosin-2 isoform X1
XM_014113203.2	XP_013968678.1	myosin-2 isoform X1
XM_005619532.3	XP_005619589.1	myosin-2 isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	MYH1C	NP_001107181.1
Mus musculus (house mouse)	Myh2	NP_001034634.2
Gallus gallus (chicken)	MYH1D	XP_415578.4
Gallus gallus (chicken)	MYH1E	NP_001013415.1
Bos taurus (cattle)	MYH2	NP_001159699.1
	MYH2	XP_002800336.1
Canis lupus familiaris (dog)	MYH2	NP_001070263.1
Gallus gallus (chicken)	MYH1A	NP_001013414.1
Gallus gallus (chicken)	MYH1F	XP_001231409.1
Homo sapiens (human)	MYH2	NP_001093582.1
Rattus norvegicus (Norway rat)	Myh2	NP_001128629.1
Gallus gallus (chicken)	MYH1B	NP_989559.1
Gallus gallus (chicken)	MYH1G	XP_003642358.1

Gene Ontology
Bibliography

Related articles in PubMed

The E3 ligase Ubr3 regulates Usher syndrome and MYH9 disorder proteins in the auditory organs of Drosophila and mammals.
Li T, Giagtzoglou N, Eberl DF, Jaiswal SN, Cai T, Godt D, Groves AK, Bellen HJ
eLife5(2016 06)

Regulation of myosin activation during cell-cell contact formation by Par3-Lgl antagonism: entosis without matrix detachment.
Wan Q, Liu J, Zheng Z, Zhu H, Chu X, Dong Z, Huang S, Du Q
Molecular biology of the cell23(11)2076-91(2012 Jun)

The N-terminal domain of the type 1 Ins(1,4,5)P3 receptor stably expressed in MDCK cells interacts with myosin IIA and alters epithelial cell morphology.
Hours MC, Mery L
Journal of cell science123(Pt 9)1449-59(2010 May)

Myosin IIA dependent retrograde flow drives 3D cell migration.
Shih W, Yamada S
Biophysical journal98(8)L29-31(2010 Apr)

Identification of myosin heavy chain I, IIa and IIx in canine skeletal muscles by an electrophoretic and immunoblotting study.
Smerdu V, Strbenc M, Meznaric-Petrusa M, Fazarinc G
Cells, tissues, organs180(2)106-16(2005)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ubr3 negatively regulates the mono-ubiquitination of non-muscle Myosin II, a protein associated with hearing loss in humans.
Title: The E3 ligase Ubr3 regulates Usher syndrome and MYH9 disorder proteins in the auditory organs of Drosophila and mammals.

Partitioning defective 3 homologue (Par3) and mammalian homologues of Drosophila Lethal (2) Giant Larvae (Lgl1/2), antagonize each other in modulating myosin II activation during cell-cell contact formation in Madin-Darby canine kidney cells.
Title: Regulation of myosin activation during cell-cell contact formation by Par3-Lgl antagonism: entosis without matrix detachment.

The recruitment of Ins(1,4,5)P(3)Rs at the apex of the lateral membrane in polarized MDCK cells, involves myosin IIA and might be important for the regulation of cortical actin dynamics.
Title: The N-terminal domain of the type 1 Ins(1,4,5)P3 receptor stably expressed in MDCK cells interacts with myosin IIA and alters epithelial cell morphology.

Myosin IIA, not myosin IIB, is required for 3D epithelial cell migration.
Title: Myosin IIA dependent retrograde flow drives 3D cell migration.

myosin heavy chain I, IIa and IIx isoforms are expressed in canine skeletal muscles [myosin heavy chain I, IIa and IIx]
Title: Identification of myosin heavy chain I, IIa and IIx in canine skeletal muscles by an electrophoretic and immunoblotting study.

The following MYH2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYH2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe05670	NM_001076795.1 Latest version!	Canis lupus familiaris myosin heavy chain 2 (MYH2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe05670	XM_005619532.3 Latest version!	Canis lupus familiaris myosin, heavy chain 2, skeletal muscle, adult (MYH2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe05670	XM_014113203.2 Latest version!	Canis lupus familiaris myosin, heavy chain 2, skeletal muscle, adult (MYH2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
View Old Accession Versions >>
OCe05670	XM_014113203.1	Canis lupus familiaris myosin, heavy chain 2, skeletal muscle, adult (MYH2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe05670	XM_005619532.2	Canis lupus familiaris myosin, heavy chain 2, skeletal muscle, adult (MYH2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MYH2 ( NM_001076795.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001070263.1 Canis lupus familiaris myosin heavy chain 2 (MYH2), mRNA.

Price & Availability↑

CloneID	OCe05670
Clone ID Related Accession (Same CDS sequence)	NM_001076795.1 , XM_005619532.2 , XM_014113203.1 , XM_014113203.2 , XM_005619532.3
Accession Version	NM_001076795.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5823bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Canis lupus familiaris(dog)
Product	myosin-2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ227280.1. On or before Oct 10, 2006 this sequence version replaced XM_536642.2, XM_857552.1, XM_857576.1, XM_857606.1, XM_857627.1, XM_857654.1, XM_857676.1, XM_857701.1, XM_857725.1, XM_857750.1, XM_857772.1, XM_857791.1, XM_857816.1, XM_857836.1, XM_857864.1, XM_857884.1, XM_857908.1, XM_857934.1, XM_857959.1, XM_857985.1, XM_858012.1, XM_846006.1, XM_858059.1, XM_858075.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA103936001, SAMEA103936002 [ECO:0000350] ##Evidence-Data-END##

ATGAGTTCAG ACCAGGAAAT GGCCATTTTC GGGGAGGCGG CTCCATACCT CCGGAAGTCT 
GAAAAGGAGC GCATTGAGGC CCAGAATAGG CCCTTTGATG CCAAGACGTC AGTCTTTGTG 
GCGGAGCCCA AAGAATCTTT TGTGAAAGGG ACTGTCCAGA GCAGAGAAGG AGGGAAAGTG 
ACAGTGAAGA CCGAAGCGGG AGCAACTCTG ACGGTGAAAG AGGACCAAGT CTTCCCCATG 
AACCCTCCCA AGTATGACAA GATCGAGGAC ATGGCCATGA TGACTCACCT GCACGAGCCT 
GCTGTGCTGT ACAACCTCAA AGAGCGTTAT GCAGCCTGGA TGATCTACAC CTATTCAGGC 
CTCTTCTGCG TCACTGTCAA CCCCTACAAG TGGCTGCCGG TGTACAACCC CGAGGTGGTG 
ACGGCCTACC GAGGCAAGAA GCGCCAGGAG GCCCCACCCC ACATCTTCTC CATCTCTGAC 
AATGCCTATC AGTTTATGCT GACTGACCGA GAGAATCAGT CAATCCTGAT CACTGGAGAA 
TCTGGTGCCG GGAAGACCGT GAACACCAAG CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTCACTGGAG AGAAGAAGAA GGAGGAAGCT ACTTCTGGCA AAATGCAGGG GACCCTTGAA 
GATCAGATCA TCAGTGCCAA CCCCCTACTG GAGGCCTTTG GCAACGCCAA GACTGTGAGG 
AATGACAACT CCTCCCGCTT TGGTAAATTC ATCAGAATTC ACTTTGGCAC TACAGGGAAG 
CTGGCTTCTG CTGATATTGA AACATATCTG TTAGAAAAGT CTAGAGTTAC TTTCCAGCTT 
AAGGCTGAAA GAAGCTATCA TATTTTTTAT CAGATCACAT CAAACAAGAA ACCAGAACTA 
ATTGAAATGC TTCTGATCAC CACCAACCCA TATGACTACC CATTTGTCAG TCAAGGGGAG 
ATCAGTGTGG CTAGCATTGA TGATCAAGAA GAGCTGATAG CAACAGATAG TGCTATTGAT 
ATTTTGGGCT TCACCAATGA AGAGAAAGTC TCCATCTACA AGCTCACGGG GGCCGTAATG 
CATTATGGTA ACTTGAAGTT CAAGCAGAAG CAGCGGGAGG AGCAGGCTGA GCCAGATGGC 
ACTGAAGTTG CTGACAAAGC AGCCTACCTC CAGAGCCTGA ACTCTGCTGA TCTGCTCAAA 
GCCCTCTGCT ACCCCAGGGT CAAGGTCGGC AATGAGTTTG TCACCAAAGG CCAGACTGTA 
GAGCAGGTAA CCAACGCAGT GGGCGCTCTG GCCAAGGCTG TCTATGAGAA GATGTTCCTG 
TGGATGGTCA CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCAGGCA GTACTTCATC 
GGGGTCTTGG ACATCGCTGG CTTTGAGATA TTTGATTTCA ACAGCCTGGA GCAGCTGTGT 
ATCAACTTCA CCAACGAGAA GCTCCAACAG TTCTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAGAAGGA GGGCATCGAG TGGACCTTCA TTGACTTCGG GATGGACCTG 
GCTGCCTGCA TCGAGCTCAT TGAGAAGCCC ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CCAAGGCCAC AGACACCTCC TTCAAGAACA AGCTGTATGA ACAGCATCTT 
GGAAAGTCTG CCAACTTCCA GAAGCCCAAG GTGGTCAAGG GCAAGGCCGA AGCACACTTC 
TCACTAATCC ACTACGCCGG CGTTGTGGAC TACAATATAA CTGGCTGGCT TGACAAGAAC 
AAGGACCCTC TCAATGAGAC TGTGGTTGGG CTGTACCAGA AGTCTTCTAT GAAAACTCTA 
GCTTACCTCT TCTCTGGGGC TCAAACTGCC GAAGCAGAGG CAAGTGGTGG TGCCAAGAAA 
GGTGGCAAGA AGAAGGGCTC TTCTTTCCAG ACGGTGTCTG CCCTTTTCAG GGAGAATCTG 
AACAAGCTGA TGACCAACCT CAGGAGTACC CATCCTCATT TTGTGCGCTG TATCATCCCC 
AATGAAACAA AAACTCCTGG GGCCATGGAG CATGAACTTG TCCTGCACCA GCTGCGGTGT 
AATGGTGTGC TGGAAGGGAT CCGCATCTGC CGGAAGGGGT TCCCAAGCAG GATCCTTTAT 
GCAGACTTCA AACAGAGATA CAAGGTATTA AATGCAAGTG CTATCCCAGA AGGACAGTTC 
ATTGACAGCA AGAAGGCTTC TGAGAAGCTC CTTGCATCCA TCGACATTGA CCACACCCAG 
TATAAATTTG GTCACACCAA GGTCTTTTTC AAAGCTGGTC TTCTGGGGCT CCTAGAGGAG 
ATGCGAGATG ACAAGCTAGC CCAGCTGATT ACCCGAACCC AGGCCAGGTG CAGAGGGTTC 
TTGGCAAGAG TGGAGTACCA GAAGATGGTG GAGAGAAGGG AGTCCATCTT CTGCATCCAG 
TATAACATCC GTGCCTTCAT GAATGTCAAG CACTGGCCCT GGATGAAGCT ATTCTTCAAG 
ATCAAACCCC TCCTCAAGAG CGCAGAGACC GAGAAGGAGA TGGCCACCAT GAAGGAAGAG 
TTTCAGAAAA CCAAAGATGA GCTTGCCAAA TCAGAGGCGA AAAGGAAGGA ACTGGAGGAA 
AAAATGGTCA CTCTCTTAAA AGAGAAAAAT GACCTGCAGC TCCAAGTTCA GGCTGAAGCT 
GAAGGCTTGG CTGATGCAGA GGAAAGATGT GACCAGCTGA TCAAAACCAA AATCCAGCTG 
GAGGCCAAGA TTAAGGAGGT GACTGAGAGA GCAGAGGATG AGGAGGAGAT CAATGCTGAG 
CTGACGGCCA AGAAGAGGAA ACTGGAAGAC GAGTGTTCAG AGCTCAAGAA AGATATTGAT 
GACCTTGAGC TGACCCTGGC CAAGGTTGAA AAGGAGAAGC ATGCCACAGA GAACAAGGTG 
AAAAACCTCA CTGAGGAGAT GGCAGGCCTG GATGAAACCA TTGCAAAGCT GACAAAGGAG 
AAGAAAGCCC TCCAAGAGGC CCACCAGCAG ACCCTGGATG ACCTACAGGC AGAAGAGGAC 
AAAGTCAACA CCCTGACCAA AGCTAAAATC AAGCTAGAGC AGCAAGTGGA TGACCTTGAA 
GGGTCCTTGG AGCAAGAAAA GAAACTTCGC ATGGATCTAG AAAGGGCTAA GAGGAAACTT 
GAAGGTGACC TCAAGTTGGC CCAAGAGTCC ATAATGGACA TTGAGAATGA AAAACAACAA 
CTTGATGAAA AGCTCAAAAA GAAAGAGTTT GAAATGAGCA ATCTGCAAAG CAAGATTGAA 
GACGAACAAG CACTTGGCAT TCAGCTGCAG AAGAAGATCA AAGAGCTGCA AGCCCGCATC 
GAGGAGCTGG AGGAGGAAAT CGAGGCAGAG CGCGCCTCCA GGGCCAAAGC AGAGAAGCAG 
CGCTCTGACC TCTCCCGGGA ACTGGAGGAG ATCAGTGAGC GCCTGGAAGA AGCTGGCGGG 
GCCACATCTG CCCAGATTGA GATGAACAAG AAGCGGGAGG CCGAGTTCCA GAAGATGCGC 
AGGGACCTGG AGGAGGCCAC CCTGCAGCAC GAAGCCACAG CAGCTACACT GAGAAAGAAG 
CACGCGGACA GCGTGGCCGA GCTCGGGGAG CAGATAGACA ACCTGCAGAG GGTCAAGCAG 
AAGCTGGAGA AGGAGAAGAG TGAGATGAAG ATGGAGATCG ATGATCTTGC CAGTAACGTA 
GAAACAGTTT CTAAAGCCAA GGGAAATCTA GAGAAAATGT GCCGCACTCT GGAGGACCAA 
GTGAGTGAGC TGAAATCAAA GGAGGAGGAG CAGCAGCGGC TGATCAATGA CCTGACCACC 
CAGAGGGGAC GCTTGCAGAC CGAATCTGGT GAATTTTCAC GACAGCTAGA TGAAAAAGAA 
GCTCTGGTGT CTCAGCTATC AAGGGGCAAA CTAGCATTTA CACAACAGAT TGAGGAATTA 
AAGAGGCAGC TGGAAGAAGA GATAAAGGCC AAGAACGCCC TGGCCCACGC CCTGCAGTCC 
TCCCGCCACG ACTGTGACCT GCTGCGGGAA CAGTATGAGG AGGAGCAGGA GTCCAAGGCC 
GAGCTGCAGA GGGCGCTGTC CAAGGCCAAC AGCGAGGTGG CCCAGTGGAG GACCAAATAT 
GAGACGGACG CCATCCAGCG CACGGAGGAG CTGGAGGAAG CAAAGAAGAA GCTGGCCCAG 
CGGTTGCAGG CGGCTGAGGA GCACGTAGAA GCTGTGAACG CCAAATGTGC TTCCCTGGAG 
AAGACAAAGC AACGGCTCCA GAATGAGGTG GAGGACCTCA TGCTGGACGT GGAGAGAACC 
AACGCAGCCT GCGCCGCCCT GGACAAGAAG CAGAGGAACT TCGACAAGAT CCTGGCAGAG 
TGGAAACAGA AGTATGAGGA AACCCATGCT GAACTCGAGG CCTCCCAGAA GGAGGCCCGC 
TCTCTCGGTA CTGAGCTGTT CAAGATGAAG AATGCCTACG AGGAGTCCTT GGATCAGCTA 
GAAACTTTGA AACGAGAGAA CAAAAACTTG CAGCAGGAGA TCTCTGACCT CACTGAGCAG 
ATTGCAGAGG GAGGGAAACG TATCCATGAA CTGGAAAAGA TAAAGAAACA AGTGGAACAA 
GAAAAGTCTG AAATTCAGGC TGCCTTGGAG GAAGCAGAGG CATCTCTTGA ACATGAAGAG 
GGAAAGATTC TGCGCATCCA GCTGGAGCTG AACCAAGTCA AGTCTGAGAT TGACAGGAAA 
ATCGCAGAAA AGGATGAGGA AATTGACCAG CTGAAGAGAA ACCACATCAG GGTTGTGGAG 
TCGATGCAGA CCATGCTGGA TGCCGAGATC AGGAGCAGGA ATGATGCCAT CAGGCTCAAG 
AAGAAGATGG AGGGAGACCT CAATGAGATG GAAATCCAGC TGAACCATGC CAACCGCATG 
GCTGCAGAGG CCCTGAGGAA CTACAGAAAT ACCCAAGGCA TCCTCAAAGA CACCCAGATC 
CACCTGGATG ACGCCCTCCG GGGCCAGGAG GACCTGAAGG AGCAGTTGGC CATGGTAGAG 
CGCAGAGCCA ACCTGCTTCA GGCTGAGATT GAGGAGCTGA GGGCCACTCT GGAGCAGACG 
GAGAGGAGCA GGAAAATCGC AGAACAGGAG CTCCTGGATG CCAGTGAGCG CGTCCAGCTC 
CTCCATACCC AGAACACTAG CCTGATCAAC ACCAAGAAGA AGCTGGAGAC AGACATTTCC 
CAGATCCAGG GAGAGATGGA GGACATTATC CAGGAAGCCC GCAATGCAGA GGAGAAGGCC 
AAGAAGGCCA TCACCGATGC GGCCATGATG GCCGAGGAGC TGAAGAAGGA GCAGGACACC 
AGCGCCCACC TGGAGCGGAT GAAGAAGAAC ATGGAGCAGA CGGTGAAGGA CCTGCAGCAT 
CGTCTGGATG AGGCTGAGCA GCTGGCCCTG AAGGGCGGGA AGAAACAGAT CCAGAAACTG 
GAGGCCAGGG TGCGTGAACT GGAAGGAGAG GTTGAGAGTG AGCAAAAGCG TAACGCGGAA 
GCTGTGAAAG GTCTGCGGAA ACACGAGAGG CGAGTAAAGG AACTCACTTA CCAGACGGAA 
GAAGATCGGA AAAATATTCT CAGACTTCAG GATTTGGTAG ATAAACTTCA AGCCAAAGTG 
AAATCTTACA AGAGACAAGC TGAGGAGGCT GAGGAACAAT CCAACACAAA TCTATCTAAA 
TTCCGCAAGC TCCAGCATGA GCTGGAGGAG GCTGAGGAGC GGGCTGACAT TGCCGAGTCC 
CAGGTCAACA AGTTGCGGGT GAAGAGCCGA GAGGTTCACA CGAAAGTCAT AAGTGAAGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001070263.1
CDS	1..5823
Translation	MSSDQEMAIFGEAAPYLRKSEKERIEAQNRPFDAKTSVFVAEPKESFVKGTVQSREGGKVTVKTEAGATLTVKEDQVFPMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNPEVVTAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEATSGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSYHIFYQITSNKKPELIEMLLITTNPYDYPFVSQGEISVASIDDQEELIATDSAIDILGFTNEEKVSIYKLTGAVMHYGNLKFKQKQREEQAEPDGTEVADKAAYLQSLNSADLLKALCYPRVKVGNEFVTKGQTVEQVTNAVGALAKAVYEKMFLWMVTRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYEQHLGKSANFQKPKVVKGKAEAHFSLIHYAGVVDYNITGWLDKNKDPLNETVVGLYQKSSMKTLAYLFSGAQTAEAEASGGAKKGGKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQFIDSKKASEKLLASIDIDHTQYKFGHTKVFFKAGLLGLLEEMRDDKLAQLITRTQARCRGFLARVEYQKMVERRESIFCIQYNIRAFMNVKHWPWMKLFFKIKPLLKSAETEKEMATMKEEFQKTKDELAKSEAKRKELEEKMVTLLKEKNDLQLQVQAEAEGLADAEERCDQLIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDETIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKIKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAQESIMDIENEKQQLDEKLKKKEFEMSNLQSKIEDEQALGIQLQKKIKELQARIEELEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEEATLQHEATAATLRKKHADSVAELGEQIDNLQRVKQKLEKEKSEMKMEIDDLASNVETVSKAKGNLEKMCRTLEDQVSELKSKEEEQQRLINDLTTQRGRLQTESGEFSRQLDEKEALVSQLSRGKLAFTQQIEELKRQLEEEIKAKNALAHALQSSRHDCDLLREQYEEEQESKAELQRALSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQAAEEHVEAVNAKCASLEKTKQRLQNEVEDLMLDVERTNAACAALDKKQRNFDKILAEWKQKYEETHAELEASQKEARSLGTELFKMKNAYEESLDQLETLKRENKNLQQEISDLTEQIAEGGKRIHELEKIKKQVEQEKSEIQAALEEAEASLEHEEGKILRIQLELNQVKSEIDRKIAEKDEEIDQLKRNHIRVVESMQTMLDAEIRSRNDAIRLKKKMEGDLNEMEIQLNHANRMAAEALRNYRNTQGILKDTQIHLDDALRGQEDLKEQLAMVERRANLLQAEIEELRATLEQTERSRKIAEQELLDASERVQLLHTQNTSLINTKKKLETDISQIQGEMEDIIQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTVKDLQHRLDEAEQLALKGGKKQIQKLEARVRELEGEVESEQKRNAEAVKGLRKHERRVKELTYQTEEDRKNILRLQDLVDKLQAKVKSYKRQAEEAEEQSNTNLSKFRKLQHELEEAEERADIAESQVNKLRVKSREVHTKVISEE

Target ORF information:

RefSeq Version	NM_001076795.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris myosin heavy chain 2 (MYH2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001076795.1

ORF Insert Sequence:

ATGAGTTCAG ACCAGGAAAT GGCCATTTTC GGGGAGGCGG CTCCATACCT CCGGAAGTCT 
GAAAAGGAGC GCATTGAGGC CCAGAATAGG CCCTTTGATG CCAAGACGTC AGTCTTTGTG 
GCGGAGCCCA AAGAATCTTT TGTGAAAGGG ACTGTCCAGA GCAGAGAAGG AGGGAAAGTG 
ACAGTGAAGA CCGAAGCGGG AGCAACTCTG ACGGTGAAAG AGGACCAAGT CTTCCCCATG 
AACCCTCCCA AGTATGACAA GATCGAGGAC ATGGCCATGA TGACTCACCT GCACGAGCCT 
GCTGTGCTGT ACAACCTCAA AGAGCGTTAT GCAGCCTGGA TGATCTACAC CTATTCAGGC 
CTCTTCTGCG TCACTGTCAA CCCCTACAAG TGGCTGCCGG TGTACAACCC CGAGGTGGTG 
ACGGCCTACC GAGGCAAGAA GCGCCAGGAG GCCCCACCCC ACATCTTCTC CATCTCTGAC 
AATGCCTATC AGTTTATGCT GACTGACCGA GAGAATCAGT CAATCCTGAT CACTGGAGAA 
TCTGGTGCCG GGAAGACCGT GAACACCAAG CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTCACTGGAG AGAAGAAGAA GGAGGAAGCT ACTTCTGGCA AAATGCAGGG GACCCTTGAA 
GATCAGATCA TCAGTGCCAA CCCCCTACTG GAGGCCTTTG GCAACGCCAA GACTGTGAGG 
AATGACAACT CCTCCCGCTT TGGTAAATTC ATCAGAATTC ACTTTGGCAC TACAGGGAAG 
CTGGCTTCTG CTGATATTGA AACATATCTG TTAGAAAAGT CTAGAGTTAC TTTCCAGCTT 
AAGGCTGAAA GAAGCTATCA TATTTTTTAT CAGATCACAT CAAACAAGAA ACCAGAACTA 
ATTGAAATGC TTCTGATCAC CACCAACCCA TATGACTACC CATTTGTCAG TCAAGGGGAG 
ATCAGTGTGG CTAGCATTGA TGATCAAGAA GAGCTGATAG CAACAGATAG TGCTATTGAT 
ATTTTGGGCT TCACCAATGA AGAGAAAGTC TCCATCTACA AGCTCACGGG GGCCGTAATG 
CATTATGGTA ACTTGAAGTT CAAGCAGAAG CAGCGGGAGG AGCAGGCTGA GCCAGATGGC 
ACTGAAGTTG CTGACAAAGC AGCCTACCTC CAGAGCCTGA ACTCTGCTGA TCTGCTCAAA 
GCCCTCTGCT ACCCCAGGGT CAAGGTCGGC AATGAGTTTG TCACCAAAGG CCAGACTGTA 
GAGCAGGTAA CCAACGCAGT GGGCGCTCTG GCCAAGGCTG TCTATGAGAA GATGTTCCTG 
TGGATGGTCA CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCAGGCA GTACTTCATC 
GGGGTCTTGG ACATCGCTGG CTTTGAGATA TTTGATTTCA ACAGCCTGGA GCAGCTGTGT 
ATCAACTTCA CCAACGAGAA GCTCCAACAG TTCTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAGAAGGA GGGCATCGAG TGGACCTTCA TTGACTTCGG GATGGACCTG 
GCTGCCTGCA TCGAGCTCAT TGAGAAGCCC ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CCAAGGCCAC AGACACCTCC TTCAAGAACA AGCTGTATGA ACAGCATCTT 
GGAAAGTCTG CCAACTTCCA GAAGCCCAAG GTGGTCAAGG GCAAGGCCGA AGCACACTTC 
TCACTAATCC ACTACGCCGG CGTTGTGGAC TACAATATAA CTGGCTGGCT TGACAAGAAC 
AAGGACCCTC TCAATGAGAC TGTGGTTGGG CTGTACCAGA AGTCTTCTAT GAAAACTCTA 
GCTTACCTCT TCTCTGGGGC TCAAACTGCC GAAGCAGAGG CAAGTGGTGG TGCCAAGAAA 
GGTGGCAAGA AGAAGGGCTC TTCTTTCCAG ACGGTGTCTG CCCTTTTCAG GGAGAATCTG 
AACAAGCTGA TGACCAACCT CAGGAGTACC CATCCTCATT TTGTGCGCTG TATCATCCCC 
AATGAAACAA AAACTCCTGG GGCCATGGAG CATGAACTTG TCCTGCACCA GCTGCGGTGT 
AATGGTGTGC TGGAAGGGAT CCGCATCTGC CGGAAGGGGT TCCCAAGCAG GATCCTTTAT 
GCAGACTTCA AACAGAGATA CAAGGTATTA AATGCAAGTG CTATCCCAGA AGGACAGTTC 
ATTGACAGCA AGAAGGCTTC TGAGAAGCTC CTTGCATCCA TCGACATTGA CCACACCCAG 
TATAAATTTG GTCACACCAA GGTCTTTTTC AAAGCTGGTC TTCTGGGGCT CCTAGAGGAG 
ATGCGAGATG ACAAGCTAGC CCAGCTGATT ACCCGAACCC AGGCCAGGTG CAGAGGGTTC 
TTGGCAAGAG TGGAGTACCA GAAGATGGTG GAGAGAAGGG AGTCCATCTT CTGCATCCAG 
TATAACATCC GTGCCTTCAT GAATGTCAAG CACTGGCCCT GGATGAAGCT ATTCTTCAAG 
ATCAAACCCC TCCTCAAGAG CGCAGAGACC GAGAAGGAGA TGGCCACCAT GAAGGAAGAG 
TTTCAGAAAA CCAAAGATGA GCTTGCCAAA TCAGAGGCGA AAAGGAAGGA ACTGGAGGAA 
AAAATGGTCA CTCTCTTAAA AGAGAAAAAT GACCTGCAGC TCCAAGTTCA GGCTGAAGCT 
GAAGGCTTGG CTGATGCAGA GGAAAGATGT GACCAGCTGA TCAAAACCAA AATCCAGCTG 
GAGGCCAAGA TTAAGGAGGT GACTGAGAGA GCAGAGGATG AGGAGGAGAT CAATGCTGAG 
CTGACGGCCA AGAAGAGGAA ACTGGAAGAC GAGTGTTCAG AGCTCAAGAA AGATATTGAT 
GACCTTGAGC TGACCCTGGC CAAGGTTGAA AAGGAGAAGC ATGCCACAGA GAACAAGGTG 
AAAAACCTCA CTGAGGAGAT GGCAGGCCTG GATGAAACCA TTGCAAAGCT GACAAAGGAG 
AAGAAAGCCC TCCAAGAGGC CCACCAGCAG ACCCTGGATG ACCTACAGGC AGAAGAGGAC 
AAAGTCAACA CCCTGACCAA AGCTAAAATC AAGCTAGAGC AGCAAGTGGA TGACCTTGAA 
GGGTCCTTGG AGCAAGAAAA GAAACTTCGC ATGGATCTAG AAAGGGCTAA GAGGAAACTT 
GAAGGTGACC TCAAGTTGGC CCAAGAGTCC ATAATGGACA TTGAGAATGA AAAACAACAA 
CTTGATGAAA AGCTCAAAAA GAAAGAGTTT GAAATGAGCA ATCTGCAAAG CAAGATTGAA 
GACGAACAAG CACTTGGCAT TCAGCTGCAG AAGAAGATCA AAGAGCTGCA AGCCCGCATC 
GAGGAGCTGG AGGAGGAAAT CGAGGCAGAG CGCGCCTCCA GGGCCAAAGC AGAGAAGCAG 
CGCTCTGACC TCTCCCGGGA ACTGGAGGAG ATCAGTGAGC GCCTGGAAGA AGCTGGCGGG 
GCCACATCTG CCCAGATTGA GATGAACAAG AAGCGGGAGG CCGAGTTCCA GAAGATGCGC 
AGGGACCTGG AGGAGGCCAC CCTGCAGCAC GAAGCCACAG CAGCTACACT GAGAAAGAAG 
CACGCGGACA GCGTGGCCGA GCTCGGGGAG CAGATAGACA ACCTGCAGAG GGTCAAGCAG 
AAGCTGGAGA AGGAGAAGAG TGAGATGAAG ATGGAGATCG ATGATCTTGC CAGTAACGTA 
GAAACAGTTT CTAAAGCCAA GGGAAATCTA GAGAAAATGT GCCGCACTCT GGAGGACCAA 
GTGAGTGAGC TGAAATCAAA GGAGGAGGAG CAGCAGCGGC TGATCAATGA CCTGACCACC 
CAGAGGGGAC GCTTGCAGAC CGAATCTGGT GAATTTTCAC GACAGCTAGA TGAAAAAGAA 
GCTCTGGTGT CTCAGCTATC AAGGGGCAAA CTAGCATTTA CACAACAGAT TGAGGAATTA 
AAGAGGCAGC TGGAAGAAGA GATAAAGGCC AAGAACGCCC TGGCCCACGC CCTGCAGTCC 
TCCCGCCACG ACTGTGACCT GCTGCGGGAA CAGTATGAGG AGGAGCAGGA GTCCAAGGCC 
GAGCTGCAGA GGGCGCTGTC CAAGGCCAAC AGCGAGGTGG CCCAGTGGAG GACCAAATAT 
GAGACGGACG CCATCCAGCG CACGGAGGAG CTGGAGGAAG CAAAGAAGAA GCTGGCCCAG 
CGGTTGCAGG CGGCTGAGGA GCACGTAGAA GCTGTGAACG CCAAATGTGC TTCCCTGGAG 
AAGACAAAGC AACGGCTCCA GAATGAGGTG GAGGACCTCA TGCTGGACGT GGAGAGAACC 
AACGCAGCCT GCGCCGCCCT GGACAAGAAG CAGAGGAACT TCGACAAGAT CCTGGCAGAG 
TGGAAACAGA AGTATGAGGA AACCCATGCT GAACTCGAGG CCTCCCAGAA GGAGGCCCGC 
TCTCTCGGTA CTGAGCTGTT CAAGATGAAG AATGCCTACG AGGAGTCCTT GGATCAGCTA 
GAAACTTTGA AACGAGAGAA CAAAAACTTG CAGCAGGAGA TCTCTGACCT CACTGAGCAG 
ATTGCAGAGG GAGGGAAACG TATCCATGAA CTGGAAAAGA TAAAGAAACA AGTGGAACAA 
GAAAAGTCTG AAATTCAGGC TGCCTTGGAG GAAGCAGAGG CATCTCTTGA ACATGAAGAG 
GGAAAGATTC TGCGCATCCA GCTGGAGCTG AACCAAGTCA AGTCTGAGAT TGACAGGAAA 
ATCGCAGAAA AGGATGAGGA AATTGACCAG CTGAAGAGAA ACCACATCAG GGTTGTGGAG 
TCGATGCAGA CCATGCTGGA TGCCGAGATC AGGAGCAGGA ATGATGCCAT CAGGCTCAAG 
AAGAAGATGG AGGGAGACCT CAATGAGATG GAAATCCAGC TGAACCATGC CAACCGCATG 
GCTGCAGAGG CCCTGAGGAA CTACAGAAAT ACCCAAGGCA TCCTCAAAGA CACCCAGATC 
CACCTGGATG ACGCCCTCCG GGGCCAGGAG GACCTGAAGG AGCAGTTGGC CATGGTAGAG 
CGCAGAGCCA ACCTGCTTCA GGCTGAGATT GAGGAGCTGA GGGCCACTCT GGAGCAGACG 
GAGAGGAGCA GGAAAATCGC AGAACAGGAG CTCCTGGATG CCAGTGAGCG CGTCCAGCTC 
CTCCATACCC AGAACACTAG CCTGATCAAC ACCAAGAAGA AGCTGGAGAC AGACATTTCC 
CAGATCCAGG GAGAGATGGA GGACATTATC CAGGAAGCCC GCAATGCAGA GGAGAAGGCC 
AAGAAGGCCA TCACCGATGC GGCCATGATG GCCGAGGAGC TGAAGAAGGA GCAGGACACC 
AGCGCCCACC TGGAGCGGAT GAAGAAGAAC ATGGAGCAGA CGGTGAAGGA CCTGCAGCAT 
CGTCTGGATG AGGCTGAGCA GCTGGCCCTG AAGGGCGGGA AGAAACAGAT CCAGAAACTG 
GAGGCCAGGG TGCGTGAACT GGAAGGAGAG GTTGAGAGTG AGCAAAAGCG TAACGCGGAA 
GCTGTGAAAG GTCTGCGGAA ACACGAGAGG CGAGTAAAGG AACTCACTTA CCAGACGGAA 
GAAGATCGGA AAAATATTCT CAGACTTCAG GATTTGGTAG ATAAACTTCA AGCCAAAGTG 
AAATCTTACA AGAGACAAGC TGAGGAGGCT GAGGAACAAT CCAACACAAA TCTATCTAAA 
TTCCGCAAGC TCCAGCATGA GCTGGAGGAG GCTGAGGAGC GGGCTGACAT TGCCGAGTCC 
CAGGTCAACA AGTTGCGGGT GAAGAGCCGA GAGGTTCACA CGAAAGTCAT AAGTGAAGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH2 ( XM_005619532.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005619589.1 Canis lupus familiaris myosin, heavy chain 2, skeletal muscle, adult (MYH2), transcript variant X1, mRNA.

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CloneID	OCe05670
Clone ID Related Accession (Same CDS sequence)	NM_001076795.1 , XM_005619532.2 , XM_014113203.1 , XM_014113203.2 , XM_005619532.3
Accession Version	XM_005619532.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5823bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	myosin-2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005619532.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTCAG ACCAGGAAAT GGCCATTTTC GGGGAGGCGG CTCCATACCT CCGGAAGTCT 
GAAAAGGAGC GCATTGAGGC CCAGAATAGG CCCTTTGATG CCAAGACGTC AGTCTTTGTG 
GCGGAGCCCA AAGAATCTTT TGTGAAAGGG ACTGTCCAGA GCAGAGAAGG AGGGAAAGTG 
ACAGTGAAGA CCGAAGCGGG AGCAACTCTG ACGGTGAAAG AGGACCAAGT CTTCCCCATG 
AACCCTCCCA AGTATGACAA GATCGAGGAC ATGGCCATGA TGACTCACCT GCACGAGCCT 
GCTGTGCTGT ACAACCTCAA AGAGCGTTAT GCAGCCTGGA TGATCTACAC CTATTCAGGC 
CTCTTCTGCG TCACTGTCAA CCCCTACAAG TGGCTGCCGG TGTACAACCC CGAGGTGGTG 
ACGGCCTACC GAGGCAAGAA GCGCCAGGAG GCCCCACCCC ACATCTTCTC CATCTCTGAC 
AATGCCTATC AGTTTATGCT GACTGACCGA GAGAATCAGT CAATCCTGAT CACTGGAGAA 
TCTGGTGCCG GGAAGACCGT GAACACCAAG CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTCACTGGAG AGAAGAAGAA GGAGGAAGCT ACTTCTGGCA AAATGCAGGG GACCCTTGAA 
GATCAGATCA TCAGTGCCAA CCCCCTACTG GAGGCCTTTG GCAACGCCAA GACTGTGAGG 
AATGACAACT CCTCCCGCTT TGGTAAATTC ATCAGAATTC ACTTTGGCAC TACAGGGAAG 
CTGGCTTCTG CTGATATTGA AACATATCTG TTAGAAAAGT CTAGAGTTAC TTTCCAGCTT 
AAGGCTGAAA GAAGCTATCA TATTTTTTAT CAGATCACAT CAAACAAGAA ACCAGAACTA 
ATTGAAATGC TTCTGATCAC CACCAACCCA TATGACTACC CATTTGTCAG TCAAGGGGAG 
ATCAGTGTGG CTAGCATTGA TGATCAAGAA GAGCTGATAG CAACAGATAG TGCTATTGAT 
ATTTTGGGCT TCACCAATGA AGAGAAAGTC TCCATCTACA AGCTCACGGG GGCCGTAATG 
CATTATGGTA ACTTGAAGTT CAAGCAGAAG CAGCGGGAGG AGCAGGCTGA GCCAGATGGC 
ACTGAAGTTG CTGACAAAGC AGCCTACCTC CAGAGCCTGA ACTCTGCTGA TCTGCTCAAA 
GCCCTCTGCT ACCCCAGGGT CAAGGTCGGC AATGAGTTTG TCACCAAAGG CCAGACTGTA 
GAGCAGGTAA CCAACGCAGT GGGCGCTCTG GCCAAGGCTG TCTATGAGAA GATGTTCCTG 
TGGATGGTCA CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCAGGCA GTACTTCATC 
GGGGTCTTGG ACATCGCTGG CTTTGAGATA TTTGATTTCA ACAGCCTGGA GCAGCTGTGT 
ATCAACTTCA CCAACGAGAA GCTCCAACAG TTCTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAGAAGGA GGGCATCGAG TGGACCTTCA TTGACTTCGG GATGGACCTG 
GCTGCCTGCA TCGAGCTCAT TGAGAAGCCC ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CCAAGGCCAC AGACACCTCC TTCAAGAACA AGCTGTATGA ACAGCATCTT 
GGAAAGTCTG CCAACTTCCA GAAGCCCAAG GTGGTCAAGG GCAAGGCCGA AGCACACTTC 
TCACTAATCC ACTACGCCGG CGTTGTGGAC TACAATATAA CTGGCTGGCT TGACAAGAAC 
AAGGACCCTC TCAATGAGAC TGTGGTTGGG CTGTACCAGA AGTCTTCTAT GAAAACTCTA 
GCTTACCTCT TCTCTGGGGC TCAAACTGCC GAAGCAGAGG CAAGTGGTGG TGCCAAGAAA 
GGTGGCAAGA AGAAGGGCTC TTCTTTCCAG ACGGTGTCTG CCCTTTTCAG GGAGAATCTG 
AACAAGCTGA TGACCAACCT CAGGAGTACC CATCCTCATT TTGTGCGCTG TATCATCCCC 
AATGAAACAA AAACTCCTGG GGCCATGGAG CATGAACTTG TCCTGCACCA GCTGCGGTGT 
AATGGTGTGC TGGAAGGGAT CCGCATCTGC CGGAAGGGGT TCCCAAGCAG GATCCTTTAT 
GCAGACTTCA AACAGAGATA CAAGGTATTA AATGCAAGTG CTATCCCAGA AGGACAGTTC 
ATTGACAGCA AGAAGGCTTC TGAGAAGCTC CTTGCATCCA TCGACATTGA CCACACCCAG 
TATAAATTTG GTCACACCAA GGTCTTTTTC AAAGCTGGTC TTCTGGGGCT CCTAGAGGAG 
ATGCGAGATG ACAAGCTAGC CCAGCTGATT ACCCGAACCC AGGCCAGGTG CAGAGGGTTC 
TTGGCAAGAG TGGAGTACCA GAAGATGGTG GAGAGAAGGG AGTCCATCTT CTGCATCCAG 
TATAACATCC GTGCCTTCAT GAATGTCAAG CACTGGCCCT GGATGAAGCT ATTCTTCAAG 
ATCAAACCCC TCCTCAAGAG CGCAGAGACC GAGAAGGAGA TGGCCACCAT GAAGGAAGAG 
TTTCAGAAAA CCAAAGATGA GCTTGCCAAA TCAGAGGCGA AAAGGAAGGA ACTGGAGGAA 
AAAATGGTCA CTCTCTTAAA AGAGAAAAAT GACCTGCAGC TCCAAGTTCA GGCTGAAGCT 
GAAGGCTTGG CTGATGCAGA GGAAAGATGT GACCAGCTGA TCAAAACCAA AATCCAGCTG 
GAGGCCAAGA TTAAGGAGGT GACTGAGAGA GCAGAGGATG AGGAGGAGAT CAATGCTGAG 
CTGACGGCCA AGAAGAGGAA ACTGGAAGAC GAGTGTTCAG AGCTCAAGAA AGATATTGAT 
GACCTTGAGC TGACCCTGGC CAAGGTTGAA AAGGAGAAGC ATGCCACAGA GAACAAGGTG 
AAAAACCTCA CTGAGGAGAT GGCAGGCCTG GATGAAACCA TTGCAAAGCT GACAAAGGAG 
AAGAAAGCCC TCCAAGAGGC CCACCAGCAG ACCCTGGATG ACCTACAGGC AGAAGAGGAC 
AAAGTCAACA CCCTGACCAA AGCTAAAATC AAGCTAGAGC AGCAAGTGGA TGACCTTGAA 
GGGTCCTTGG AGCAAGAAAA GAAACTTCGC ATGGATCTAG AAAGGGCTAA GAGGAAACTT 
GAAGGTGACC TCAAGTTGGC CCAAGAGTCC ATAATGGACA TTGAGAATGA AAAACAACAA 
CTTGATGAAA AGCTCAAAAA GAAAGAGTTT GAAATGAGCA ATCTGCAAAG CAAGATTGAA 
GACGAACAAG CACTTGGCAT TCAGCTGCAG AAGAAGATCA AAGAGCTGCA AGCCCGCATC 
GAGGAGCTGG AGGAGGAAAT CGAGGCAGAG CGCGCCTCCA GGGCCAAAGC AGAGAAGCAG 
CGCTCTGACC TCTCCCGGGA ACTGGAGGAG ATCAGTGAGC GCCTGGAAGA AGCTGGCGGG 
GCCACATCTG CCCAGATTGA GATGAACAAG AAGCGGGAGG CCGAGTTCCA GAAGATGCGC 
AGGGACCTGG AGGAGGCCAC CCTGCAGCAC GAAGCCACAG CAGCTACACT GAGAAAGAAG 
CACGCGGACA GCGTGGCCGA GCTCGGGGAG CAGATAGACA ACCTGCAGAG GGTCAAGCAG 
AAGCTGGAGA AGGAGAAGAG TGAGATGAAG ATGGAGATCG ATGATCTTGC CAGTAACGTA 
GAAACAGTTT CTAAAGCCAA GGGAAATCTA GAGAAAATGT GCCGCACTCT GGAGGACCAA 
GTGAGTGAGC TGAAATCAAA GGAGGAGGAG CAGCAGCGGC TGATCAATGA CCTGACCACC 
CAGAGGGGAC GCTTGCAGAC CGAATCTGGT GAATTTTCAC GACAGCTAGA TGAAAAAGAA 
GCTCTGGTGT CTCAGCTATC AAGGGGCAAA CTAGCATTTA CACAACAGAT TGAGGAATTA 
AAGAGGCAGC TGGAAGAAGA GATAAAGGCC AAGAACGCCC TGGCCCACGC CCTGCAGTCC 
TCCCGCCACG ACTGTGACCT GCTGCGGGAA CAGTATGAGG AGGAGCAGGA GTCCAAGGCC 
GAGCTGCAGA GGGCGCTGTC CAAGGCCAAC AGCGAGGTGG CCCAGTGGAG GACCAAATAT 
GAGACGGACG CCATCCAGCG CACGGAGGAG CTGGAGGAAG CAAAGAAGAA GCTGGCCCAG 
CGGTTGCAGG CGGCTGAGGA GCACGTAGAA GCTGTGAACG CCAAATGTGC TTCCCTGGAG 
AAGACAAAGC AACGGCTCCA GAATGAGGTG GAGGACCTCA TGCTGGACGT GGAGAGAACC 
AACGCAGCCT GCGCCGCCCT GGACAAGAAG CAGAGGAACT TCGACAAGAT CCTGGCAGAG 
TGGAAACAGA AGTATGAGGA AACCCATGCT GAACTCGAGG CCTCCCAGAA GGAGGCCCGC 
TCTCTCGGTA CTGAGCTGTT CAAGATGAAG AATGCCTACG AGGAGTCCTT GGATCAGCTA 
GAAACTTTGA AACGAGAGAA CAAAAACTTG CAGCAGGAGA TCTCTGACCT CACTGAGCAG 
ATTGCAGAGG GAGGGAAACG TATCCATGAA CTGGAAAAGA TAAAGAAACA AGTGGAACAA 
GAAAAGTCTG AAATTCAGGC TGCCTTGGAG GAAGCAGAGG CATCTCTTGA ACATGAAGAG 
GGAAAGATTC TGCGCATCCA GCTGGAGCTG AACCAAGTCA AGTCTGAGAT TGACAGGAAA 
ATCGCAGAAA AGGATGAGGA AATTGACCAG CTGAAGAGAA ACCACATCAG GGTTGTGGAG 
TCGATGCAGA CCATGCTGGA TGCCGAGATC AGGAGCAGGA ATGATGCCAT CAGGCTCAAG 
AAGAAGATGG AGGGAGACCT CAATGAGATG GAAATCCAGC TGAACCATGC CAACCGCATG 
GCTGCAGAGG CCCTGAGGAA CTACAGAAAT ACCCAAGGCA TCCTCAAAGA CACCCAGATC 
CACCTGGATG ACGCCCTCCG GGGCCAGGAG GACCTGAAGG AGCAGTTGGC CATGGTAGAG 
CGCAGAGCCA ACCTGCTTCA GGCTGAGATT GAGGAGCTGA GGGCCACTCT GGAGCAGACG 
GAGAGGAGCA GGAAAATCGC AGAACAGGAG CTCCTGGATG CCAGTGAGCG CGTCCAGCTC 
CTCCATACCC AGAACACTAG CCTGATCAAC ACCAAGAAGA AGCTGGAGAC AGACATTTCC 
CAGATCCAGG GAGAGATGGA GGACATTATC CAGGAAGCCC GCAATGCAGA GGAGAAGGCC 
AAGAAGGCCA TCACCGATGC GGCCATGATG GCCGAGGAGC TGAAGAAGGA GCAGGACACC 
AGCGCCCACC TGGAGCGGAT GAAGAAGAAC ATGGAGCAGA CGGTGAAGGA CCTGCAGCAT 
CGTCTGGATG AGGCTGAGCA GCTGGCCCTG AAGGGCGGGA AGAAACAGAT CCAGAAACTG 
GAGGCCAGGG TGCGTGAACT GGAAGGAGAG GTTGAGAGTG AGCAAAAGCG TAACGCGGAA 
GCTGTGAAAG GTCTGCGGAA ACACGAGAGG CGAGTAAAGG AACTCACTTA CCAGACGGAA 
GAAGATCGGA AAAATATTCT CAGACTTCAG GATTTGGTAG ATAAACTTCA AGCCAAAGTG 
AAATCTTACA AGAGACAAGC TGAGGAGGCT GAGGAACAAT CCAACACAAA TCTATCTAAA 
TTCCGCAAGC TCCAGCATGA GCTGGAGGAG GCTGAGGAGC GGGCTGACAT TGCCGAGTCC 
CAGGTCAACA AGTTGCGGGT GAAGAGCCGA GAGGTTCACA CGAAAGTCAT AAGTGAAGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005619589.1
CDS	157..5979
Translation	MSSDQEMAIFGEAAPYLRKSEKERIEAQNRPFDAKTSVFVAEPKESFVKGTVQSREGGKVTVKTEAGATLTVKEDQVFPMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNPEVVTAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEATSGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSYHIFYQITSNKKPELIEMLLITTNPYDYPFVSQGEISVASIDDQEELIATDSAIDILGFTNEEKVSIYKLTGAVMHYGNLKFKQKQREEQAEPDGTEVADKAAYLQSLNSADLLKALCYPRVKVGNEFVTKGQTVEQVTNAVGALAKAVYEKMFLWMVTRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYEQHLGKSANFQKPKVVKGKAEAHFSLIHYAGVVDYNITGWLDKNKDPLNETVVGLYQKSSMKTLAYLFSGAQTAEAEASGGAKKGGKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQFIDSKKASEKLLASIDIDHTQYKFGHTKVFFKAGLLGLLEEMRDDKLAQLITRTQARCRGFLARVEYQKMVERRESIFCIQYNIRAFMNVKHWPWMKLFFKIKPLLKSAETEKEMATMKEEFQKTKDELAKSEAKRKELEEKMVTLLKEKNDLQLQVQAEAEGLADAEERCDQLIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDETIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKIKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAQESIMDIENEKQQLDEKLKKKEFEMSNLQSKIEDEQALGIQLQKKIKELQARIEELEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEEATLQHEATAATLRKKHADSVAELGEQIDNLQRVKQKLEKEKSEMKMEIDDLASNVETVSKAKGNLEKMCRTLEDQVSELKSKEEEQQRLINDLTTQRGRLQTESGEFSRQLDEKEALVSQLSRGKLAFTQQIEELKRQLEEEIKAKNALAHALQSSRHDCDLLREQYEEEQESKAELQRALSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQAAEEHVEAVNAKCASLEKTKQRLQNEVEDLMLDVERTNAACAALDKKQRNFDKILAEWKQKYEETHAELEASQKEARSLGTELFKMKNAYEESLDQLETLKRENKNLQQEISDLTEQIAEGGKRIHELEKIKKQVEQEKSEIQAALEEAEASLEHEEGKILRIQLELNQVKSEIDRKIAEKDEEIDQLKRNHIRVVESMQTMLDAEIRSRNDAIRLKKKMEGDLNEMEIQLNHANRMAAEALRNYRNTQGILKDTQIHLDDALRGQEDLKEQLAMVERRANLLQAEIEELRATLEQTERSRKIAEQELLDASERVQLLHTQNTSLINTKKKLETDISQIQGEMEDIIQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTVKDLQHRLDEAEQLALKGGKKQIQKLEARVRELEGEVESEQKRNAEAVKGLRKHERRVKELTYQTEEDRKNILRLQDLVDKLQAKVKSYKRQAEEAEEQSNTNLSKFRKLQHELEEAEERADIAESQVNKLRVKSREVHTKVISEE

Target ORF information:

RefSeq Version	XM_005619532.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris myosin, heavy chain 2, skeletal muscle, adult (MYH2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005619532.2

ORF Insert Sequence:

ATGAGTTCAG ACCAGGAAAT GGCCATTTTC GGGGAGGCGG CTCCATACCT CCGGAAGTCT 
GAAAAGGAGC GCATTGAGGC CCAGAATAGG CCCTTTGATG CCAAGACGTC AGTCTTTGTG 
GCGGAGCCCA AAGAATCTTT TGTGAAAGGG ACTGTCCAGA GCAGAGAAGG AGGGAAAGTG 
ACAGTGAAGA CCGAAGCGGG AGCAACTCTG ACGGTGAAAG AGGACCAAGT CTTCCCCATG 
AACCCTCCCA AGTATGACAA GATCGAGGAC ATGGCCATGA TGACTCACCT GCACGAGCCT 
GCTGTGCTGT ACAACCTCAA AGAGCGTTAT GCAGCCTGGA TGATCTACAC CTATTCAGGC 
CTCTTCTGCG TCACTGTCAA CCCCTACAAG TGGCTGCCGG TGTACAACCC CGAGGTGGTG 
ACGGCCTACC GAGGCAAGAA GCGCCAGGAG GCCCCACCCC ACATCTTCTC CATCTCTGAC 
AATGCCTATC AGTTTATGCT GACTGACCGA GAGAATCAGT CAATCCTGAT CACTGGAGAA 
TCTGGTGCCG GGAAGACCGT GAACACCAAG CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTCACTGGAG AGAAGAAGAA GGAGGAAGCT ACTTCTGGCA AAATGCAGGG GACCCTTGAA 
GATCAGATCA TCAGTGCCAA CCCCCTACTG GAGGCCTTTG GCAACGCCAA GACTGTGAGG 
AATGACAACT CCTCCCGCTT TGGTAAATTC ATCAGAATTC ACTTTGGCAC TACAGGGAAG 
CTGGCTTCTG CTGATATTGA AACATATCTG TTAGAAAAGT CTAGAGTTAC TTTCCAGCTT 
AAGGCTGAAA GAAGCTATCA TATTTTTTAT CAGATCACAT CAAACAAGAA ACCAGAACTA 
ATTGAAATGC TTCTGATCAC CACCAACCCA TATGACTACC CATTTGTCAG TCAAGGGGAG 
ATCAGTGTGG CTAGCATTGA TGATCAAGAA GAGCTGATAG CAACAGATAG TGCTATTGAT 
ATTTTGGGCT TCACCAATGA AGAGAAAGTC TCCATCTACA AGCTCACGGG GGCCGTAATG 
CATTATGGTA ACTTGAAGTT CAAGCAGAAG CAGCGGGAGG AGCAGGCTGA GCCAGATGGC 
ACTGAAGTTG CTGACAAAGC AGCCTACCTC CAGAGCCTGA ACTCTGCTGA TCTGCTCAAA 
GCCCTCTGCT ACCCCAGGGT CAAGGTCGGC AATGAGTTTG TCACCAAAGG CCAGACTGTA 
GAGCAGGTAA CCAACGCAGT GGGCGCTCTG GCCAAGGCTG TCTATGAGAA GATGTTCCTG 
TGGATGGTCA CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCAGGCA GTACTTCATC 
GGGGTCTTGG ACATCGCTGG CTTTGAGATA TTTGATTTCA ACAGCCTGGA GCAGCTGTGT 
ATCAACTTCA CCAACGAGAA GCTCCAACAG TTCTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAGAAGGA GGGCATCGAG TGGACCTTCA TTGACTTCGG GATGGACCTG 
GCTGCCTGCA TCGAGCTCAT TGAGAAGCCC ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CCAAGGCCAC AGACACCTCC TTCAAGAACA AGCTGTATGA ACAGCATCTT 
GGAAAGTCTG CCAACTTCCA GAAGCCCAAG GTGGTCAAGG GCAAGGCCGA AGCACACTTC 
TCACTAATCC ACTACGCCGG CGTTGTGGAC TACAATATAA CTGGCTGGCT TGACAAGAAC 
AAGGACCCTC TCAATGAGAC TGTGGTTGGG CTGTACCAGA AGTCTTCTAT GAAAACTCTA 
GCTTACCTCT TCTCTGGGGC TCAAACTGCC GAAGCAGAGG CAAGTGGTGG TGCCAAGAAA 
GGTGGCAAGA AGAAGGGCTC TTCTTTCCAG ACGGTGTCTG CCCTTTTCAG GGAGAATCTG 
AACAAGCTGA TGACCAACCT CAGGAGTACC CATCCTCATT TTGTGCGCTG TATCATCCCC 
AATGAAACAA AAACTCCTGG GGCCATGGAG CATGAACTTG TCCTGCACCA GCTGCGGTGT 
AATGGTGTGC TGGAAGGGAT CCGCATCTGC CGGAAGGGGT TCCCAAGCAG GATCCTTTAT 
GCAGACTTCA AACAGAGATA CAAGGTATTA AATGCAAGTG CTATCCCAGA AGGACAGTTC 
ATTGACAGCA AGAAGGCTTC TGAGAAGCTC CTTGCATCCA TCGACATTGA CCACACCCAG 
TATAAATTTG GTCACACCAA GGTCTTTTTC AAAGCTGGTC TTCTGGGGCT CCTAGAGGAG 
ATGCGAGATG ACAAGCTAGC CCAGCTGATT ACCCGAACCC AGGCCAGGTG CAGAGGGTTC 
TTGGCAAGAG TGGAGTACCA GAAGATGGTG GAGAGAAGGG AGTCCATCTT CTGCATCCAG 
TATAACATCC GTGCCTTCAT GAATGTCAAG CACTGGCCCT GGATGAAGCT ATTCTTCAAG 
ATCAAACCCC TCCTCAAGAG CGCAGAGACC GAGAAGGAGA TGGCCACCAT GAAGGAAGAG 
TTTCAGAAAA CCAAAGATGA GCTTGCCAAA TCAGAGGCGA AAAGGAAGGA ACTGGAGGAA 
AAAATGGTCA CTCTCTTAAA AGAGAAAAAT GACCTGCAGC TCCAAGTTCA GGCTGAAGCT 
GAAGGCTTGG CTGATGCAGA GGAAAGATGT GACCAGCTGA TCAAAACCAA AATCCAGCTG 
GAGGCCAAGA TTAAGGAGGT GACTGAGAGA GCAGAGGATG AGGAGGAGAT CAATGCTGAG 
CTGACGGCCA AGAAGAGGAA ACTGGAAGAC GAGTGTTCAG AGCTCAAGAA AGATATTGAT 
GACCTTGAGC TGACCCTGGC CAAGGTTGAA AAGGAGAAGC ATGCCACAGA GAACAAGGTG 
AAAAACCTCA CTGAGGAGAT GGCAGGCCTG GATGAAACCA TTGCAAAGCT GACAAAGGAG 
AAGAAAGCCC TCCAAGAGGC CCACCAGCAG ACCCTGGATG ACCTACAGGC AGAAGAGGAC 
AAAGTCAACA CCCTGACCAA AGCTAAAATC AAGCTAGAGC AGCAAGTGGA TGACCTTGAA 
GGGTCCTTGG AGCAAGAAAA GAAACTTCGC ATGGATCTAG AAAGGGCTAA GAGGAAACTT 
GAAGGTGACC TCAAGTTGGC CCAAGAGTCC ATAATGGACA TTGAGAATGA AAAACAACAA 
CTTGATGAAA AGCTCAAAAA GAAAGAGTTT GAAATGAGCA ATCTGCAAAG CAAGATTGAA 
GACGAACAAG CACTTGGCAT TCAGCTGCAG AAGAAGATCA AAGAGCTGCA AGCCCGCATC 
GAGGAGCTGG AGGAGGAAAT CGAGGCAGAG CGCGCCTCCA GGGCCAAAGC AGAGAAGCAG 
CGCTCTGACC TCTCCCGGGA ACTGGAGGAG ATCAGTGAGC GCCTGGAAGA AGCTGGCGGG 
GCCACATCTG CCCAGATTGA GATGAACAAG AAGCGGGAGG CCGAGTTCCA GAAGATGCGC 
AGGGACCTGG AGGAGGCCAC CCTGCAGCAC GAAGCCACAG CAGCTACACT GAGAAAGAAG 
CACGCGGACA GCGTGGCCGA GCTCGGGGAG CAGATAGACA ACCTGCAGAG GGTCAAGCAG 
AAGCTGGAGA AGGAGAAGAG TGAGATGAAG ATGGAGATCG ATGATCTTGC CAGTAACGTA 
GAAACAGTTT CTAAAGCCAA GGGAAATCTA GAGAAAATGT GCCGCACTCT GGAGGACCAA 
GTGAGTGAGC TGAAATCAAA GGAGGAGGAG CAGCAGCGGC TGATCAATGA CCTGACCACC 
CAGAGGGGAC GCTTGCAGAC CGAATCTGGT GAATTTTCAC GACAGCTAGA TGAAAAAGAA 
GCTCTGGTGT CTCAGCTATC AAGGGGCAAA CTAGCATTTA CACAACAGAT TGAGGAATTA 
AAGAGGCAGC TGGAAGAAGA GATAAAGGCC AAGAACGCCC TGGCCCACGC CCTGCAGTCC 
TCCCGCCACG ACTGTGACCT GCTGCGGGAA CAGTATGAGG AGGAGCAGGA GTCCAAGGCC 
GAGCTGCAGA GGGCGCTGTC CAAGGCCAAC AGCGAGGTGG CCCAGTGGAG GACCAAATAT 
GAGACGGACG CCATCCAGCG CACGGAGGAG CTGGAGGAAG CAAAGAAGAA GCTGGCCCAG 
CGGTTGCAGG CGGCTGAGGA GCACGTAGAA GCTGTGAACG CCAAATGTGC TTCCCTGGAG 
AAGACAAAGC AACGGCTCCA GAATGAGGTG GAGGACCTCA TGCTGGACGT GGAGAGAACC 
AACGCAGCCT GCGCCGCCCT GGACAAGAAG CAGAGGAACT TCGACAAGAT CCTGGCAGAG 
TGGAAACAGA AGTATGAGGA AACCCATGCT GAACTCGAGG CCTCCCAGAA GGAGGCCCGC 
TCTCTCGGTA CTGAGCTGTT CAAGATGAAG AATGCCTACG AGGAGTCCTT GGATCAGCTA 
GAAACTTTGA AACGAGAGAA CAAAAACTTG CAGCAGGAGA TCTCTGACCT CACTGAGCAG 
ATTGCAGAGG GAGGGAAACG TATCCATGAA CTGGAAAAGA TAAAGAAACA AGTGGAACAA 
GAAAAGTCTG AAATTCAGGC TGCCTTGGAG GAAGCAGAGG CATCTCTTGA ACATGAAGAG 
GGAAAGATTC TGCGCATCCA GCTGGAGCTG AACCAAGTCA AGTCTGAGAT TGACAGGAAA 
ATCGCAGAAA AGGATGAGGA AATTGACCAG CTGAAGAGAA ACCACATCAG GGTTGTGGAG 
TCGATGCAGA CCATGCTGGA TGCCGAGATC AGGAGCAGGA ATGATGCCAT CAGGCTCAAG 
AAGAAGATGG AGGGAGACCT CAATGAGATG GAAATCCAGC TGAACCATGC CAACCGCATG 
GCTGCAGAGG CCCTGAGGAA CTACAGAAAT ACCCAAGGCA TCCTCAAAGA CACCCAGATC 
CACCTGGATG ACGCCCTCCG GGGCCAGGAG GACCTGAAGG AGCAGTTGGC CATGGTAGAG 
CGCAGAGCCA ACCTGCTTCA GGCTGAGATT GAGGAGCTGA GGGCCACTCT GGAGCAGACG 
GAGAGGAGCA GGAAAATCGC AGAACAGGAG CTCCTGGATG CCAGTGAGCG CGTCCAGCTC 
CTCCATACCC AGAACACTAG CCTGATCAAC ACCAAGAAGA AGCTGGAGAC AGACATTTCC 
CAGATCCAGG GAGAGATGGA GGACATTATC CAGGAAGCCC GCAATGCAGA GGAGAAGGCC 
AAGAAGGCCA TCACCGATGC GGCCATGATG GCCGAGGAGC TGAAGAAGGA GCAGGACACC 
AGCGCCCACC TGGAGCGGAT GAAGAAGAAC ATGGAGCAGA CGGTGAAGGA CCTGCAGCAT 
CGTCTGGATG AGGCTGAGCA GCTGGCCCTG AAGGGCGGGA AGAAACAGAT CCAGAAACTG 
GAGGCCAGGG TGCGTGAACT GGAAGGAGAG GTTGAGAGTG AGCAAAAGCG TAACGCGGAA 
GCTGTGAAAG GTCTGCGGAA ACACGAGAGG CGAGTAAAGG AACTCACTTA CCAGACGGAA 
GAAGATCGGA AAAATATTCT CAGACTTCAG GATTTGGTAG ATAAACTTCA AGCCAAAGTG 
AAATCTTACA AGAGACAAGC TGAGGAGGCT GAGGAACAAT CCAACACAAA TCTATCTAAA 
TTCCGCAAGC TCCAGCATGA GCTGGAGGAG GCTGAGGAGC GGGCTGACAT TGCCGAGTCC 
CAGGTCAACA AGTTGCGGGT GAAGAGCCGA GAGGTTCACA CGAAAGTCAT AAGTGAAGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH2 ( XM_014113203.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013968678.1 Canis lupus familiaris myosin, heavy chain 2, skeletal muscle, adult (MYH2), transcript variant X2, mRNA.

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CloneID	OCe05670
Clone ID Related Accession (Same CDS sequence)	NM_001076795.1 , XM_005619532.2 , XM_014113203.1 , XM_014113203.2 , XM_005619532.3
Accession Version	XM_014113203.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5823bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	myosin-2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTCAG ACCAGGAAAT GGCCATTTTC GGGGAGGCGG CTCCATACCT CCGGAAGTCT 
GAAAAGGAGC GCATTGAGGC CCAGAATAGG CCCTTTGATG CCAAGACGTC AGTCTTTGTG 
GCGGAGCCCA AAGAATCTTT TGTGAAAGGG ACTGTCCAGA GCAGAGAAGG AGGGAAAGTG 
ACAGTGAAGA CCGAAGCGGG AGCAACTCTG ACGGTGAAAG AGGACCAAGT CTTCCCCATG 
AACCCTCCCA AGTATGACAA GATCGAGGAC ATGGCCATGA TGACTCACCT GCACGAGCCT 
GCTGTGCTGT ACAACCTCAA AGAGCGTTAT GCAGCCTGGA TGATCTACAC CTATTCAGGC 
CTCTTCTGCG TCACTGTCAA CCCCTACAAG TGGCTGCCGG TGTACAACCC CGAGGTGGTG 
ACGGCCTACC GAGGCAAGAA GCGCCAGGAG GCCCCACCCC ACATCTTCTC CATCTCTGAC 
AATGCCTATC AGTTTATGCT GACTGACCGA GAGAATCAGT CAATCCTGAT CACTGGAGAA 
TCTGGTGCCG GGAAGACCGT GAACACCAAG CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTCACTGGAG AGAAGAAGAA GGAGGAAGCT ACTTCTGGCA AAATGCAGGG GACCCTTGAA 
GATCAGATCA TCAGTGCCAA CCCCCTACTG GAGGCCTTTG GCAACGCCAA GACTGTGAGG 
AATGACAACT CCTCCCGCTT TGGTAAATTC ATCAGAATTC ACTTTGGCAC TACAGGGAAG 
CTGGCTTCTG CTGATATTGA AACATATCTG TTAGAAAAGT CTAGAGTTAC TTTCCAGCTT 
AAGGCTGAAA GAAGCTATCA TATTTTTTAT CAGATCACAT CAAACAAGAA ACCAGAACTA 
ATTGAAATGC TTCTGATCAC CACCAACCCA TATGACTACC CATTTGTCAG TCAAGGGGAG 
ATCAGTGTGG CTAGCATTGA TGATCAAGAA GAGCTGATAG CAACAGATAG TGCTATTGAT 
ATTTTGGGCT TCACCAATGA AGAGAAAGTC TCCATCTACA AGCTCACGGG GGCCGTAATG 
CATTATGGTA ACTTGAAGTT CAAGCAGAAG CAGCGGGAGG AGCAGGCTGA GCCAGATGGC 
ACTGAAGTTG CTGACAAAGC AGCCTACCTC CAGAGCCTGA ACTCTGCTGA TCTGCTCAAA 
GCCCTCTGCT ACCCCAGGGT CAAGGTCGGC AATGAGTTTG TCACCAAAGG CCAGACTGTA 
GAGCAGGTAA CCAACGCAGT GGGCGCTCTG GCCAAGGCTG TCTATGAGAA GATGTTCCTG 
TGGATGGTCA CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCAGGCA GTACTTCATC 
GGGGTCTTGG ACATCGCTGG CTTTGAGATA TTTGATTTCA ACAGCCTGGA GCAGCTGTGT 
ATCAACTTCA CCAACGAGAA GCTCCAACAG TTCTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAGAAGGA GGGCATCGAG TGGACCTTCA TTGACTTCGG GATGGACCTG 
GCTGCCTGCA TCGAGCTCAT TGAGAAGCCC ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CCAAGGCCAC AGACACCTCC TTCAAGAACA AGCTGTATGA ACAGCATCTT 
GGAAAGTCTG CCAACTTCCA GAAGCCCAAG GTGGTCAAGG GCAAGGCCGA AGCACACTTC 
TCACTAATCC ACTACGCCGG CGTTGTGGAC TACAATATAA CTGGCTGGCT TGACAAGAAC 
AAGGACCCTC TCAATGAGAC TGTGGTTGGG CTGTACCAGA AGTCTTCTAT GAAAACTCTA 
GCTTACCTCT TCTCTGGGGC TCAAACTGCC GAAGCAGAGG CAAGTGGTGG TGCCAAGAAA 
GGTGGCAAGA AGAAGGGCTC TTCTTTCCAG ACGGTGTCTG CCCTTTTCAG GGAGAATCTG 
AACAAGCTGA TGACCAACCT CAGGAGTACC CATCCTCATT TTGTGCGCTG TATCATCCCC 
AATGAAACAA AAACTCCTGG GGCCATGGAG CATGAACTTG TCCTGCACCA GCTGCGGTGT 
AATGGTGTGC TGGAAGGGAT CCGCATCTGC CGGAAGGGGT TCCCAAGCAG GATCCTTTAT 
GCAGACTTCA AACAGAGATA CAAGGTATTA AATGCAAGTG CTATCCCAGA AGGACAGTTC 
ATTGACAGCA AGAAGGCTTC TGAGAAGCTC CTTGCATCCA TCGACATTGA CCACACCCAG 
TATAAATTTG GTCACACCAA GGTCTTTTTC AAAGCTGGTC TTCTGGGGCT CCTAGAGGAG 
ATGCGAGATG ACAAGCTAGC CCAGCTGATT ACCCGAACCC AGGCCAGGTG CAGAGGGTTC 
TTGGCAAGAG TGGAGTACCA GAAGATGGTG GAGAGAAGGG AGTCCATCTT CTGCATCCAG 
TATAACATCC GTGCCTTCAT GAATGTCAAG CACTGGCCCT GGATGAAGCT ATTCTTCAAG 
ATCAAACCCC TCCTCAAGAG CGCAGAGACC GAGAAGGAGA TGGCCACCAT GAAGGAAGAG 
TTTCAGAAAA CCAAAGATGA GCTTGCCAAA TCAGAGGCGA AAAGGAAGGA ACTGGAGGAA 
AAAATGGTCA CTCTCTTAAA AGAGAAAAAT GACCTGCAGC TCCAAGTTCA GGCTGAAGCT 
GAAGGCTTGG CTGATGCAGA GGAAAGATGT GACCAGCTGA TCAAAACCAA AATCCAGCTG 
GAGGCCAAGA TTAAGGAGGT GACTGAGAGA GCAGAGGATG AGGAGGAGAT CAATGCTGAG 
CTGACGGCCA AGAAGAGGAA ACTGGAAGAC GAGTGTTCAG AGCTCAAGAA AGATATTGAT 
GACCTTGAGC TGACCCTGGC CAAGGTTGAA AAGGAGAAGC ATGCCACAGA GAACAAGGTG 
AAAAACCTCA CTGAGGAGAT GGCAGGCCTG GATGAAACCA TTGCAAAGCT GACAAAGGAG 
AAGAAAGCCC TCCAAGAGGC CCACCAGCAG ACCCTGGATG ACCTACAGGC AGAAGAGGAC 
AAAGTCAACA CCCTGACCAA AGCTAAAATC AAGCTAGAGC AGCAAGTGGA TGACCTTGAA 
GGGTCCTTGG AGCAAGAAAA GAAACTTCGC ATGGATCTAG AAAGGGCTAA GAGGAAACTT 
GAAGGTGACC TCAAGTTGGC CCAAGAGTCC ATAATGGACA TTGAGAATGA AAAACAACAA 
CTTGATGAAA AGCTCAAAAA GAAAGAGTTT GAAATGAGCA ATCTGCAAAG CAAGATTGAA 
GACGAACAAG CACTTGGCAT TCAGCTGCAG AAGAAGATCA AAGAGCTGCA AGCCCGCATC 
GAGGAGCTGG AGGAGGAAAT CGAGGCAGAG CGCGCCTCCA GGGCCAAAGC AGAGAAGCAG 
CGCTCTGACC TCTCCCGGGA ACTGGAGGAG ATCAGTGAGC GCCTGGAAGA AGCTGGCGGG 
GCCACATCTG CCCAGATTGA GATGAACAAG AAGCGGGAGG CCGAGTTCCA GAAGATGCGC 
AGGGACCTGG AGGAGGCCAC CCTGCAGCAC GAAGCCACAG CAGCTACACT GAGAAAGAAG 
CACGCGGACA GCGTGGCCGA GCTCGGGGAG CAGATAGACA ACCTGCAGAG GGTCAAGCAG 
AAGCTGGAGA AGGAGAAGAG TGAGATGAAG ATGGAGATCG ATGATCTTGC CAGTAACGTA 
GAAACAGTTT CTAAAGCCAA GGGAAATCTA GAGAAAATGT GCCGCACTCT GGAGGACCAA 
GTGAGTGAGC TGAAATCAAA GGAGGAGGAG CAGCAGCGGC TGATCAATGA CCTGACCACC 
CAGAGGGGAC GCTTGCAGAC CGAATCTGGT GAATTTTCAC GACAGCTAGA TGAAAAAGAA 
GCTCTGGTGT CTCAGCTATC AAGGGGCAAA CTAGCATTTA CACAACAGAT TGAGGAATTA 
AAGAGGCAGC TGGAAGAAGA GATAAAGGCC AAGAACGCCC TGGCCCACGC CCTGCAGTCC 
TCCCGCCACG ACTGTGACCT GCTGCGGGAA CAGTATGAGG AGGAGCAGGA GTCCAAGGCC 
GAGCTGCAGA GGGCGCTGTC CAAGGCCAAC AGCGAGGTGG CCCAGTGGAG GACCAAATAT 
GAGACGGACG CCATCCAGCG CACGGAGGAG CTGGAGGAAG CAAAGAAGAA GCTGGCCCAG 
CGGTTGCAGG CGGCTGAGGA GCACGTAGAA GCTGTGAACG CCAAATGTGC TTCCCTGGAG 
AAGACAAAGC AACGGCTCCA GAATGAGGTG GAGGACCTCA TGCTGGACGT GGAGAGAACC 
AACGCAGCCT GCGCCGCCCT GGACAAGAAG CAGAGGAACT TCGACAAGAT CCTGGCAGAG 
TGGAAACAGA AGTATGAGGA AACCCATGCT GAACTCGAGG CCTCCCAGAA GGAGGCCCGC 
TCTCTCGGTA CTGAGCTGTT CAAGATGAAG AATGCCTACG AGGAGTCCTT GGATCAGCTA 
GAAACTTTGA AACGAGAGAA CAAAAACTTG CAGCAGGAGA TCTCTGACCT CACTGAGCAG 
ATTGCAGAGG GAGGGAAACG TATCCATGAA CTGGAAAAGA TAAAGAAACA AGTGGAACAA 
GAAAAGTCTG AAATTCAGGC TGCCTTGGAG GAAGCAGAGG CATCTCTTGA ACATGAAGAG 
GGAAAGATTC TGCGCATCCA GCTGGAGCTG AACCAAGTCA AGTCTGAGAT TGACAGGAAA 
ATCGCAGAAA AGGATGAGGA AATTGACCAG CTGAAGAGAA ACCACATCAG GGTTGTGGAG 
TCGATGCAGA CCATGCTGGA TGCCGAGATC AGGAGCAGGA ATGATGCCAT CAGGCTCAAG 
AAGAAGATGG AGGGAGACCT CAATGAGATG GAAATCCAGC TGAACCATGC CAACCGCATG 
GCTGCAGAGG CCCTGAGGAA CTACAGAAAT ACCCAAGGCA TCCTCAAAGA CACCCAGATC 
CACCTGGATG ACGCCCTCCG GGGCCAGGAG GACCTGAAGG AGCAGTTGGC CATGGTAGAG 
CGCAGAGCCA ACCTGCTTCA GGCTGAGATT GAGGAGCTGA GGGCCACTCT GGAGCAGACG 
GAGAGGAGCA GGAAAATCGC AGAACAGGAG CTCCTGGATG CCAGTGAGCG CGTCCAGCTC 
CTCCATACCC AGAACACTAG CCTGATCAAC ACCAAGAAGA AGCTGGAGAC AGACATTTCC 
CAGATCCAGG GAGAGATGGA GGACATTATC CAGGAAGCCC GCAATGCAGA GGAGAAGGCC 
AAGAAGGCCA TCACCGATGC GGCCATGATG GCCGAGGAGC TGAAGAAGGA GCAGGACACC 
AGCGCCCACC TGGAGCGGAT GAAGAAGAAC ATGGAGCAGA CGGTGAAGGA CCTGCAGCAT 
CGTCTGGATG AGGCTGAGCA GCTGGCCCTG AAGGGCGGGA AGAAACAGAT CCAGAAACTG 
GAGGCCAGGG TGCGTGAACT GGAAGGAGAG GTTGAGAGTG AGCAAAAGCG TAACGCGGAA 
GCTGTGAAAG GTCTGCGGAA ACACGAGAGG CGAGTAAAGG AACTCACTTA CCAGACGGAA 
GAAGATCGGA AAAATATTCT CAGACTTCAG GATTTGGTAG ATAAACTTCA AGCCAAAGTG 
AAATCTTACA AGAGACAAGC TGAGGAGGCT GAGGAACAAT CCAACACAAA TCTATCTAAA 
TTCCGCAAGC TCCAGCATGA GCTGGAGGAG GCTGAGGAGC GGGCTGACAT TGCCGAGTCC 
CAGGTCAACA AGTTGCGGGT GAAGAGCCGA GAGGTTCACA CGAAAGTCAT AAGTGAAGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013968678.1
CDS	129..5951
Translation	MSSDQEMAIFGEAAPYLRKSEKERIEAQNRPFDAKTSVFVAEPKESFVKGTVQSREGGKVTVKTEAGATLTVKEDQVFPMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNPEVVTAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEATSGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSYHIFYQITSNKKPELIEMLLITTNPYDYPFVSQGEISVASIDDQEELIATDSAIDILGFTNEEKVSIYKLTGAVMHYGNLKFKQKQREEQAEPDGTEVADKAAYLQSLNSADLLKALCYPRVKVGNEFVTKGQTVEQVTNAVGALAKAVYEKMFLWMVTRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYEQHLGKSANFQKPKVVKGKAEAHFSLIHYAGVVDYNITGWLDKNKDPLNETVVGLYQKSSMKTLAYLFSGAQTAEAEASGGAKKGGKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQFIDSKKASEKLLASIDIDHTQYKFGHTKVFFKAGLLGLLEEMRDDKLAQLITRTQARCRGFLARVEYQKMVERRESIFCIQYNIRAFMNVKHWPWMKLFFKIKPLLKSAETEKEMATMKEEFQKTKDELAKSEAKRKELEEKMVTLLKEKNDLQLQVQAEAEGLADAEERCDQLIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDETIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKIKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAQESIMDIENEKQQLDEKLKKKEFEMSNLQSKIEDEQALGIQLQKKIKELQARIEELEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEEATLQHEATAATLRKKHADSVAELGEQIDNLQRVKQKLEKEKSEMKMEIDDLASNVETVSKAKGNLEKMCRTLEDQVSELKSKEEEQQRLINDLTTQRGRLQTESGEFSRQLDEKEALVSQLSRGKLAFTQQIEELKRQLEEEIKAKNALAHALQSSRHDCDLLREQYEEEQESKAELQRALSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQAAEEHVEAVNAKCASLEKTKQRLQNEVEDLMLDVERTNAACAALDKKQRNFDKILAEWKQKYEETHAELEASQKEARSLGTELFKMKNAYEESLDQLETLKRENKNLQQEISDLTEQIAEGGKRIHELEKIKKQVEQEKSEIQAALEEAEASLEHEEGKILRIQLELNQVKSEIDRKIAEKDEEIDQLKRNHIRVVESMQTMLDAEIRSRNDAIRLKKKMEGDLNEMEIQLNHANRMAAEALRNYRNTQGILKDTQIHLDDALRGQEDLKEQLAMVERRANLLQAEIEELRATLEQTERSRKIAEQELLDASERVQLLHTQNTSLINTKKKLETDISQIQGEMEDIIQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTVKDLQHRLDEAEQLALKGGKKQIQKLEARVRELEGEVESEQKRNAEAVKGLRKHERRVKELTYQTEEDRKNILRLQDLVDKLQAKVKSYKRQAEEAEEQSNTNLSKFRKLQHELEEAEERADIAESQVNKLRVKSREVHTKVISEE

Target ORF information:

RefSeq Version	XM_014113203.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris myosin, heavy chain 2, skeletal muscle, adult (MYH2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113203.1

ORF Insert Sequence:

ATGAGTTCAG ACCAGGAAAT GGCCATTTTC GGGGAGGCGG CTCCATACCT CCGGAAGTCT 
GAAAAGGAGC GCATTGAGGC CCAGAATAGG CCCTTTGATG CCAAGACGTC AGTCTTTGTG 
GCGGAGCCCA AAGAATCTTT TGTGAAAGGG ACTGTCCAGA GCAGAGAAGG AGGGAAAGTG 
ACAGTGAAGA CCGAAGCGGG AGCAACTCTG ACGGTGAAAG AGGACCAAGT CTTCCCCATG 
AACCCTCCCA AGTATGACAA GATCGAGGAC ATGGCCATGA TGACTCACCT GCACGAGCCT 
GCTGTGCTGT ACAACCTCAA AGAGCGTTAT GCAGCCTGGA TGATCTACAC CTATTCAGGC 
CTCTTCTGCG TCACTGTCAA CCCCTACAAG TGGCTGCCGG TGTACAACCC CGAGGTGGTG 
ACGGCCTACC GAGGCAAGAA GCGCCAGGAG GCCCCACCCC ACATCTTCTC CATCTCTGAC 
AATGCCTATC AGTTTATGCT GACTGACCGA GAGAATCAGT CAATCCTGAT CACTGGAGAA 
TCTGGTGCCG GGAAGACCGT GAACACCAAG CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTCACTGGAG AGAAGAAGAA GGAGGAAGCT ACTTCTGGCA AAATGCAGGG GACCCTTGAA 
GATCAGATCA TCAGTGCCAA CCCCCTACTG GAGGCCTTTG GCAACGCCAA GACTGTGAGG 
AATGACAACT CCTCCCGCTT TGGTAAATTC ATCAGAATTC ACTTTGGCAC TACAGGGAAG 
CTGGCTTCTG CTGATATTGA AACATATCTG TTAGAAAAGT CTAGAGTTAC TTTCCAGCTT 
AAGGCTGAAA GAAGCTATCA TATTTTTTAT CAGATCACAT CAAACAAGAA ACCAGAACTA 
ATTGAAATGC TTCTGATCAC CACCAACCCA TATGACTACC CATTTGTCAG TCAAGGGGAG 
ATCAGTGTGG CTAGCATTGA TGATCAAGAA GAGCTGATAG CAACAGATAG TGCTATTGAT 
ATTTTGGGCT TCACCAATGA AGAGAAAGTC TCCATCTACA AGCTCACGGG GGCCGTAATG 
CATTATGGTA ACTTGAAGTT CAAGCAGAAG CAGCGGGAGG AGCAGGCTGA GCCAGATGGC 
ACTGAAGTTG CTGACAAAGC AGCCTACCTC CAGAGCCTGA ACTCTGCTGA TCTGCTCAAA 
GCCCTCTGCT ACCCCAGGGT CAAGGTCGGC AATGAGTTTG TCACCAAAGG CCAGACTGTA 
GAGCAGGTAA CCAACGCAGT GGGCGCTCTG GCCAAGGCTG TCTATGAGAA GATGTTCCTG 
TGGATGGTCA CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCAGGCA GTACTTCATC 
GGGGTCTTGG ACATCGCTGG CTTTGAGATA TTTGATTTCA ACAGCCTGGA GCAGCTGTGT 
ATCAACTTCA CCAACGAGAA GCTCCAACAG TTCTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAGAAGGA GGGCATCGAG TGGACCTTCA TTGACTTCGG GATGGACCTG 
GCTGCCTGCA TCGAGCTCAT TGAGAAGCCC ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CCAAGGCCAC AGACACCTCC TTCAAGAACA AGCTGTATGA ACAGCATCTT 
GGAAAGTCTG CCAACTTCCA GAAGCCCAAG GTGGTCAAGG GCAAGGCCGA AGCACACTTC 
TCACTAATCC ACTACGCCGG CGTTGTGGAC TACAATATAA CTGGCTGGCT TGACAAGAAC 
AAGGACCCTC TCAATGAGAC TGTGGTTGGG CTGTACCAGA AGTCTTCTAT GAAAACTCTA 
GCTTACCTCT TCTCTGGGGC TCAAACTGCC GAAGCAGAGG CAAGTGGTGG TGCCAAGAAA 
GGTGGCAAGA AGAAGGGCTC TTCTTTCCAG ACGGTGTCTG CCCTTTTCAG GGAGAATCTG 
AACAAGCTGA TGACCAACCT CAGGAGTACC CATCCTCATT TTGTGCGCTG TATCATCCCC 
AATGAAACAA AAACTCCTGG GGCCATGGAG CATGAACTTG TCCTGCACCA GCTGCGGTGT 
AATGGTGTGC TGGAAGGGAT CCGCATCTGC CGGAAGGGGT TCCCAAGCAG GATCCTTTAT 
GCAGACTTCA AACAGAGATA CAAGGTATTA AATGCAAGTG CTATCCCAGA AGGACAGTTC 
ATTGACAGCA AGAAGGCTTC TGAGAAGCTC CTTGCATCCA TCGACATTGA CCACACCCAG 
TATAAATTTG GTCACACCAA GGTCTTTTTC AAAGCTGGTC TTCTGGGGCT CCTAGAGGAG 
ATGCGAGATG ACAAGCTAGC CCAGCTGATT ACCCGAACCC AGGCCAGGTG CAGAGGGTTC 
TTGGCAAGAG TGGAGTACCA GAAGATGGTG GAGAGAAGGG AGTCCATCTT CTGCATCCAG 
TATAACATCC GTGCCTTCAT GAATGTCAAG CACTGGCCCT GGATGAAGCT ATTCTTCAAG 
ATCAAACCCC TCCTCAAGAG CGCAGAGACC GAGAAGGAGA TGGCCACCAT GAAGGAAGAG 
TTTCAGAAAA CCAAAGATGA GCTTGCCAAA TCAGAGGCGA AAAGGAAGGA ACTGGAGGAA 
AAAATGGTCA CTCTCTTAAA AGAGAAAAAT GACCTGCAGC TCCAAGTTCA GGCTGAAGCT 
GAAGGCTTGG CTGATGCAGA GGAAAGATGT GACCAGCTGA TCAAAACCAA AATCCAGCTG 
GAGGCCAAGA TTAAGGAGGT GACTGAGAGA GCAGAGGATG AGGAGGAGAT CAATGCTGAG 
CTGACGGCCA AGAAGAGGAA ACTGGAAGAC GAGTGTTCAG AGCTCAAGAA AGATATTGAT 
GACCTTGAGC TGACCCTGGC CAAGGTTGAA AAGGAGAAGC ATGCCACAGA GAACAAGGTG 
AAAAACCTCA CTGAGGAGAT GGCAGGCCTG GATGAAACCA TTGCAAAGCT GACAAAGGAG 
AAGAAAGCCC TCCAAGAGGC CCACCAGCAG ACCCTGGATG ACCTACAGGC AGAAGAGGAC 
AAAGTCAACA CCCTGACCAA AGCTAAAATC AAGCTAGAGC AGCAAGTGGA TGACCTTGAA 
GGGTCCTTGG AGCAAGAAAA GAAACTTCGC ATGGATCTAG AAAGGGCTAA GAGGAAACTT 
GAAGGTGACC TCAAGTTGGC CCAAGAGTCC ATAATGGACA TTGAGAATGA AAAACAACAA 
CTTGATGAAA AGCTCAAAAA GAAAGAGTTT GAAATGAGCA ATCTGCAAAG CAAGATTGAA 
GACGAACAAG CACTTGGCAT TCAGCTGCAG AAGAAGATCA AAGAGCTGCA AGCCCGCATC 
GAGGAGCTGG AGGAGGAAAT CGAGGCAGAG CGCGCCTCCA GGGCCAAAGC AGAGAAGCAG 
CGCTCTGACC TCTCCCGGGA ACTGGAGGAG ATCAGTGAGC GCCTGGAAGA AGCTGGCGGG 
GCCACATCTG CCCAGATTGA GATGAACAAG AAGCGGGAGG CCGAGTTCCA GAAGATGCGC 
AGGGACCTGG AGGAGGCCAC CCTGCAGCAC GAAGCCACAG CAGCTACACT GAGAAAGAAG 
CACGCGGACA GCGTGGCCGA GCTCGGGGAG CAGATAGACA ACCTGCAGAG GGTCAAGCAG 
AAGCTGGAGA AGGAGAAGAG TGAGATGAAG ATGGAGATCG ATGATCTTGC CAGTAACGTA 
GAAACAGTTT CTAAAGCCAA GGGAAATCTA GAGAAAATGT GCCGCACTCT GGAGGACCAA 
GTGAGTGAGC TGAAATCAAA GGAGGAGGAG CAGCAGCGGC TGATCAATGA CCTGACCACC 
CAGAGGGGAC GCTTGCAGAC CGAATCTGGT GAATTTTCAC GACAGCTAGA TGAAAAAGAA 
GCTCTGGTGT CTCAGCTATC AAGGGGCAAA CTAGCATTTA CACAACAGAT TGAGGAATTA 
AAGAGGCAGC TGGAAGAAGA GATAAAGGCC AAGAACGCCC TGGCCCACGC CCTGCAGTCC 
TCCCGCCACG ACTGTGACCT GCTGCGGGAA CAGTATGAGG AGGAGCAGGA GTCCAAGGCC 
GAGCTGCAGA GGGCGCTGTC CAAGGCCAAC AGCGAGGTGG CCCAGTGGAG GACCAAATAT 
GAGACGGACG CCATCCAGCG CACGGAGGAG CTGGAGGAAG CAAAGAAGAA GCTGGCCCAG 
CGGTTGCAGG CGGCTGAGGA GCACGTAGAA GCTGTGAACG CCAAATGTGC TTCCCTGGAG 
AAGACAAAGC AACGGCTCCA GAATGAGGTG GAGGACCTCA TGCTGGACGT GGAGAGAACC 
AACGCAGCCT GCGCCGCCCT GGACAAGAAG CAGAGGAACT TCGACAAGAT CCTGGCAGAG 
TGGAAACAGA AGTATGAGGA AACCCATGCT GAACTCGAGG CCTCCCAGAA GGAGGCCCGC 
TCTCTCGGTA CTGAGCTGTT CAAGATGAAG AATGCCTACG AGGAGTCCTT GGATCAGCTA 
GAAACTTTGA AACGAGAGAA CAAAAACTTG CAGCAGGAGA TCTCTGACCT CACTGAGCAG 
ATTGCAGAGG GAGGGAAACG TATCCATGAA CTGGAAAAGA TAAAGAAACA AGTGGAACAA 
GAAAAGTCTG AAATTCAGGC TGCCTTGGAG GAAGCAGAGG CATCTCTTGA ACATGAAGAG 
GGAAAGATTC TGCGCATCCA GCTGGAGCTG AACCAAGTCA AGTCTGAGAT TGACAGGAAA 
ATCGCAGAAA AGGATGAGGA AATTGACCAG CTGAAGAGAA ACCACATCAG GGTTGTGGAG 
TCGATGCAGA CCATGCTGGA TGCCGAGATC AGGAGCAGGA ATGATGCCAT CAGGCTCAAG 
AAGAAGATGG AGGGAGACCT CAATGAGATG GAAATCCAGC TGAACCATGC CAACCGCATG 
GCTGCAGAGG CCCTGAGGAA CTACAGAAAT ACCCAAGGCA TCCTCAAAGA CACCCAGATC 
CACCTGGATG ACGCCCTCCG GGGCCAGGAG GACCTGAAGG AGCAGTTGGC CATGGTAGAG 
CGCAGAGCCA ACCTGCTTCA GGCTGAGATT GAGGAGCTGA GGGCCACTCT GGAGCAGACG 
GAGAGGAGCA GGAAAATCGC AGAACAGGAG CTCCTGGATG CCAGTGAGCG CGTCCAGCTC 
CTCCATACCC AGAACACTAG CCTGATCAAC ACCAAGAAGA AGCTGGAGAC AGACATTTCC 
CAGATCCAGG GAGAGATGGA GGACATTATC CAGGAAGCCC GCAATGCAGA GGAGAAGGCC 
AAGAAGGCCA TCACCGATGC GGCCATGATG GCCGAGGAGC TGAAGAAGGA GCAGGACACC 
AGCGCCCACC TGGAGCGGAT GAAGAAGAAC ATGGAGCAGA CGGTGAAGGA CCTGCAGCAT 
CGTCTGGATG AGGCTGAGCA GCTGGCCCTG AAGGGCGGGA AGAAACAGAT CCAGAAACTG 
GAGGCCAGGG TGCGTGAACT GGAAGGAGAG GTTGAGAGTG AGCAAAAGCG TAACGCGGAA 
GCTGTGAAAG GTCTGCGGAA ACACGAGAGG CGAGTAAAGG AACTCACTTA CCAGACGGAA 
GAAGATCGGA AAAATATTCT CAGACTTCAG GATTTGGTAG ATAAACTTCA AGCCAAAGTG 
AAATCTTACA AGAGACAAGC TGAGGAGGCT GAGGAACAAT CCAACACAAA TCTATCTAAA 
TTCCGCAAGC TCCAGCATGA GCTGGAGGAG GCTGAGGAGC GGGCTGACAT TGCCGAGTCC 
CAGGTCAACA AGTTGCGGGT GAAGAGCCGA GAGGTTCACA CGAAAGTCAT AAGTGAAGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH2 ( XM_014113203.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013968678.1 Canis lupus familiaris myosin, heavy chain 2, skeletal muscle, adult (MYH2), transcript variant X1, mRNA.

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CloneID	OCe05670
Clone ID Related Accession (Same CDS sequence)	NM_001076795.1 , XM_005619532.2 , XM_014113203.1 , XM_014113203.2 , XM_005619532.3
Accession Version	XM_014113203.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5823bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	myosin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014113203.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTCAG ACCAGGAAAT GGCCATTTTC GGGGAGGCGG CTCCATACCT CCGGAAGTCT 
GAAAAGGAGC GCATTGAGGC CCAGAATAGG CCCTTTGATG CCAAGACGTC AGTCTTTGTG 
GCGGAGCCCA AAGAATCTTT TGTGAAAGGG ACTGTCCAGA GCAGAGAAGG AGGGAAAGTG 
ACAGTGAAGA CCGAAGCGGG AGCAACTCTG ACGGTGAAAG AGGACCAAGT CTTCCCCATG 
AACCCTCCCA AGTATGACAA GATCGAGGAC ATGGCCATGA TGACTCACCT GCACGAGCCT 
GCTGTGCTGT ACAACCTCAA AGAGCGTTAT GCAGCCTGGA TGATCTACAC CTATTCAGGC 
CTCTTCTGCG TCACTGTCAA CCCCTACAAG TGGCTGCCGG TGTACAACCC CGAGGTGGTG 
ACGGCCTACC GAGGCAAGAA GCGCCAGGAG GCCCCACCCC ACATCTTCTC CATCTCTGAC 
AATGCCTATC AGTTTATGCT GACTGACCGA GAGAATCAGT CAATCCTGAT CACTGGAGAA 
TCTGGTGCCG GGAAGACCGT GAACACCAAG CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTCACTGGAG AGAAGAAGAA GGAGGAAGCT ACTTCTGGCA AAATGCAGGG GACCCTTGAA 
GATCAGATCA TCAGTGCCAA CCCCCTACTG GAGGCCTTTG GCAACGCCAA GACTGTGAGG 
AATGACAACT CCTCCCGCTT TGGTAAATTC ATCAGAATTC ACTTTGGCAC TACAGGGAAG 
CTGGCTTCTG CTGATATTGA AACATATCTG TTAGAAAAGT CTAGAGTTAC TTTCCAGCTT 
AAGGCTGAAA GAAGCTATCA TATTTTTTAT CAGATCACAT CAAACAAGAA ACCAGAACTA 
ATTGAAATGC TTCTGATCAC CACCAACCCA TATGACTACC CATTTGTCAG TCAAGGGGAG 
ATCAGTGTGG CTAGCATTGA TGATCAAGAA GAGCTGATAG CAACAGATAG TGCTATTGAT 
ATTTTGGGCT TCACCAATGA AGAGAAAGTC TCCATCTACA AGCTCACGGG GGCCGTAATG 
CATTATGGTA ACTTGAAGTT CAAGCAGAAG CAGCGGGAGG AGCAGGCTGA GCCAGATGGC 
ACTGAAGTTG CTGACAAAGC AGCCTACCTC CAGAGCCTGA ACTCTGCTGA TCTGCTCAAA 
GCCCTCTGCT ACCCCAGGGT CAAGGTCGGC AATGAGTTTG TCACCAAAGG CCAGACTGTA 
GAGCAGGTAA CCAACGCAGT GGGCGCTCTG GCCAAGGCTG TCTATGAGAA GATGTTCCTG 
TGGATGGTCA CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCAGGCA GTACTTCATC 
GGGGTCTTGG ACATCGCTGG CTTTGAGATA TTTGATTTCA ACAGCCTGGA GCAGCTGTGT 
ATCAACTTCA CCAACGAGAA GCTCCAACAG TTCTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAGAAGGA GGGCATCGAG TGGACCTTCA TTGACTTCGG GATGGACCTG 
GCTGCCTGCA TCGAGCTCAT TGAGAAGCCC ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CCAAGGCCAC AGACACCTCC TTCAAGAACA AGCTGTATGA ACAGCATCTT 
GGAAAGTCTG CCAACTTCCA GAAGCCCAAG GTGGTCAAGG GCAAGGCCGA AGCACACTTC 
TCACTAATCC ACTACGCCGG CGTTGTGGAC TACAATATAA CTGGCTGGCT TGACAAGAAC 
AAGGACCCTC TCAATGAGAC TGTGGTTGGG CTGTACCAGA AGTCTTCTAT GAAAACTCTA 
GCTTACCTCT TCTCTGGGGC TCAAACTGCC GAAGCAGAGG CAAGTGGTGG TGCCAAGAAA 
GGTGGCAAGA AGAAGGGCTC TTCTTTCCAG ACGGTGTCTG CCCTTTTCAG GGAGAATCTG 
AACAAGCTGA TGACCAACCT CAGGAGTACC CATCCTCATT TTGTGCGCTG TATCATCCCC 
AATGAAACAA AAACTCCTGG GGCCATGGAG CATGAACTTG TCCTGCACCA GCTGCGGTGT 
AATGGTGTGC TGGAAGGGAT CCGCATCTGC CGGAAGGGGT TCCCAAGCAG GATCCTTTAT 
GCAGACTTCA AACAGAGATA CAAGGTATTA AATGCAAGTG CTATCCCAGA AGGACAGTTC 
ATTGACAGCA AGAAGGCTTC TGAGAAGCTC CTTGCATCCA TCGACATTGA CCACACCCAG 
TATAAATTTG GTCACACCAA GGTCTTTTTC AAAGCTGGTC TTCTGGGGCT CCTAGAGGAG 
ATGCGAGATG ACAAGCTAGC CCAGCTGATT ACCCGAACCC AGGCCAGGTG CAGAGGGTTC 
TTGGCAAGAG TGGAGTACCA GAAGATGGTG GAGAGAAGGG AGTCCATCTT CTGCATCCAG 
TATAACATCC GTGCCTTCAT GAATGTCAAG CACTGGCCCT GGATGAAGCT ATTCTTCAAG 
ATCAAACCCC TCCTCAAGAG CGCAGAGACC GAGAAGGAGA TGGCCACCAT GAAGGAAGAG 
TTTCAGAAAA CCAAAGATGA GCTTGCCAAA TCAGAGGCGA AAAGGAAGGA ACTGGAGGAA 
AAAATGGTCA CTCTCTTAAA AGAGAAAAAT GACCTGCAGC TCCAAGTTCA GGCTGAAGCT 
GAAGGCTTGG CTGATGCAGA GGAAAGATGT GACCAGCTGA TCAAAACCAA AATCCAGCTG 
GAGGCCAAGA TTAAGGAGGT GACTGAGAGA GCAGAGGATG AGGAGGAGAT CAATGCTGAG 
CTGACGGCCA AGAAGAGGAA ACTGGAAGAC GAGTGTTCAG AGCTCAAGAA AGATATTGAT 
GACCTTGAGC TGACCCTGGC CAAGGTTGAA AAGGAGAAGC ATGCCACAGA GAACAAGGTG 
AAAAACCTCA CTGAGGAGAT GGCAGGCCTG GATGAAACCA TTGCAAAGCT GACAAAGGAG 
AAGAAAGCCC TCCAAGAGGC CCACCAGCAG ACCCTGGATG ACCTACAGGC AGAAGAGGAC 
AAAGTCAACA CCCTGACCAA AGCTAAAATC AAGCTAGAGC AGCAAGTGGA TGACCTTGAA 
GGGTCCTTGG AGCAAGAAAA GAAACTTCGC ATGGATCTAG AAAGGGCTAA GAGGAAACTT 
GAAGGTGACC TCAAGTTGGC CCAAGAGTCC ATAATGGACA TTGAGAATGA AAAACAACAA 
CTTGATGAAA AGCTCAAAAA GAAAGAGTTT GAAATGAGCA ATCTGCAAAG CAAGATTGAA 
GACGAACAAG CACTTGGCAT TCAGCTGCAG AAGAAGATCA AAGAGCTGCA AGCCCGCATC 
GAGGAGCTGG AGGAGGAAAT CGAGGCAGAG CGCGCCTCCA GGGCCAAAGC AGAGAAGCAG 
CGCTCTGACC TCTCCCGGGA ACTGGAGGAG ATCAGTGAGC GCCTGGAAGA AGCTGGCGGG 
GCCACATCTG CCCAGATTGA GATGAACAAG AAGCGGGAGG CCGAGTTCCA GAAGATGCGC 
AGGGACCTGG AGGAGGCCAC CCTGCAGCAC GAAGCCACAG CAGCTACACT GAGAAAGAAG 
CACGCGGACA GCGTGGCCGA GCTCGGGGAG CAGATAGACA ACCTGCAGAG GGTCAAGCAG 
AAGCTGGAGA AGGAGAAGAG TGAGATGAAG ATGGAGATCG ATGATCTTGC CAGTAACGTA 
GAAACAGTTT CTAAAGCCAA GGGAAATCTA GAGAAAATGT GCCGCACTCT GGAGGACCAA 
GTGAGTGAGC TGAAATCAAA GGAGGAGGAG CAGCAGCGGC TGATCAATGA CCTGACCACC 
CAGAGGGGAC GCTTGCAGAC CGAATCTGGT GAATTTTCAC GACAGCTAGA TGAAAAAGAA 
GCTCTGGTGT CTCAGCTATC AAGGGGCAAA CTAGCATTTA CACAACAGAT TGAGGAATTA 
AAGAGGCAGC TGGAAGAAGA GATAAAGGCC AAGAACGCCC TGGCCCACGC CCTGCAGTCC 
TCCCGCCACG ACTGTGACCT GCTGCGGGAA CAGTATGAGG AGGAGCAGGA GTCCAAGGCC 
GAGCTGCAGA GGGCGCTGTC CAAGGCCAAC AGCGAGGTGG CCCAGTGGAG GACCAAATAT 
GAGACGGACG CCATCCAGCG CACGGAGGAG CTGGAGGAAG CAAAGAAGAA GCTGGCCCAG 
CGGTTGCAGG CGGCTGAGGA GCACGTAGAA GCTGTGAACG CCAAATGTGC TTCCCTGGAG 
AAGACAAAGC AACGGCTCCA GAATGAGGTG GAGGACCTCA TGCTGGACGT GGAGAGAACC 
AACGCAGCCT GCGCCGCCCT GGACAAGAAG CAGAGGAACT TCGACAAGAT CCTGGCAGAG 
TGGAAACAGA AGTATGAGGA AACCCATGCT GAACTCGAGG CCTCCCAGAA GGAGGCCCGC 
TCTCTCGGTA CTGAGCTGTT CAAGATGAAG AATGCCTACG AGGAGTCCTT GGATCAGCTA 
GAAACTTTGA AACGAGAGAA CAAAAACTTG CAGCAGGAGA TCTCTGACCT CACTGAGCAG 
ATTGCAGAGG GAGGGAAACG TATCCATGAA CTGGAAAAGA TAAAGAAACA AGTGGAACAA 
GAAAAGTCTG AAATTCAGGC TGCCTTGGAG GAAGCAGAGG CATCTCTTGA ACATGAAGAG 
GGAAAGATTC TGCGCATCCA GCTGGAGCTG AACCAAGTCA AGTCTGAGAT TGACAGGAAA 
ATCGCAGAAA AGGATGAGGA AATTGACCAG CTGAAGAGAA ACCACATCAG GGTTGTGGAG 
TCGATGCAGA CCATGCTGGA TGCCGAGATC AGGAGCAGGA ATGATGCCAT CAGGCTCAAG 
AAGAAGATGG AGGGAGACCT CAATGAGATG GAAATCCAGC TGAACCATGC CAACCGCATG 
GCTGCAGAGG CCCTGAGGAA CTACAGAAAT ACCCAAGGCA TCCTCAAAGA CACCCAGATC 
CACCTGGATG ACGCCCTCCG GGGCCAGGAG GACCTGAAGG AGCAGTTGGC CATGGTAGAG 
CGCAGAGCCA ACCTGCTTCA GGCTGAGATT GAGGAGCTGA GGGCCACTCT GGAGCAGACG 
GAGAGGAGCA GGAAAATCGC AGAACAGGAG CTCCTGGATG CCAGTGAGCG CGTCCAGCTC 
CTCCATACCC AGAACACTAG CCTGATCAAC ACCAAGAAGA AGCTGGAGAC AGACATTTCC 
CAGATCCAGG GAGAGATGGA GGACATTATC CAGGAAGCCC GCAATGCAGA GGAGAAGGCC 
AAGAAGGCCA TCACCGATGC GGCCATGATG GCCGAGGAGC TGAAGAAGGA GCAGGACACC 
AGCGCCCACC TGGAGCGGAT GAAGAAGAAC ATGGAGCAGA CGGTGAAGGA CCTGCAGCAT 
CGTCTGGATG AGGCTGAGCA GCTGGCCCTG AAGGGCGGGA AGAAACAGAT CCAGAAACTG 
GAGGCCAGGG TGCGTGAACT GGAAGGAGAG GTTGAGAGTG AGCAAAAGCG TAACGCGGAA 
GCTGTGAAAG GTCTGCGGAA ACACGAGAGG CGAGTAAAGG AACTCACTTA CCAGACGGAA 
GAAGATCGGA AAAATATTCT CAGACTTCAG GATTTGGTAG ATAAACTTCA AGCCAAAGTG 
AAATCTTACA AGAGACAAGC TGAGGAGGCT GAGGAACAAT CCAACACAAA TCTATCTAAA 
TTCCGCAAGC TCCAGCATGA GCTGGAGGAG GCTGAGGAGC GGGCTGACAT TGCCGAGTCC 
CAGGTCAACA AGTTGCGGGT GAAGAGCCGA GAGGTTCACA CGAAAGTCAT AAGTGAAGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013968678.1
CDS	129..5951
Translation	MSSDQEMAIFGEAAPYLRKSEKERIEAQNRPFDAKTSVFVAEPKESFVKGTVQSREGGKVTVKTEAGATLTVKEDQVFPMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNPEVVTAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEATSGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSYHIFYQITSNKKPELIEMLLITTNPYDYPFVSQGEISVASIDDQEELIATDSAIDILGFTNEEKVSIYKLTGAVMHYGNLKFKQKQREEQAEPDGTEVADKAAYLQSLNSADLLKALCYPRVKVGNEFVTKGQTVEQVTNAVGALAKAVYEKMFLWMVTRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYEQHLGKSANFQKPKVVKGKAEAHFSLIHYAGVVDYNITGWLDKNKDPLNETVVGLYQKSSMKTLAYLFSGAQTAEAEASGGAKKGGKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQFIDSKKASEKLLASIDIDHTQYKFGHTKVFFKAGLLGLLEEMRDDKLAQLITRTQARCRGFLARVEYQKMVERRESIFCIQYNIRAFMNVKHWPWMKLFFKIKPLLKSAETEKEMATMKEEFQKTKDELAKSEAKRKELEEKMVTLLKEKNDLQLQVQAEAEGLADAEERCDQLIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDETIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKIKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAQESIMDIENEKQQLDEKLKKKEFEMSNLQSKIEDEQALGIQLQKKIKELQARIEELEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEEATLQHEATAATLRKKHADSVAELGEQIDNLQRVKQKLEKEKSEMKMEIDDLASNVETVSKAKGNLEKMCRTLEDQVSELKSKEEEQQRLINDLTTQRGRLQTESGEFSRQLDEKEALVSQLSRGKLAFTQQIEELKRQLEEEIKAKNALAHALQSSRHDCDLLREQYEEEQESKAELQRALSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQAAEEHVEAVNAKCASLEKTKQRLQNEVEDLMLDVERTNAACAALDKKQRNFDKILAEWKQKYEETHAELEASQKEARSLGTELFKMKNAYEESLDQLETLKRENKNLQQEISDLTEQIAEGGKRIHELEKIKKQVEQEKSEIQAALEEAEASLEHEEGKILRIQLELNQVKSEIDRKIAEKDEEIDQLKRNHIRVVESMQTMLDAEIRSRNDAIRLKKKMEGDLNEMEIQLNHANRMAAEALRNYRNTQGILKDTQIHLDDALRGQEDLKEQLAMVERRANLLQAEIEELRATLEQTERSRKIAEQELLDASERVQLLHTQNTSLINTKKKLETDISQIQGEMEDIIQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTVKDLQHRLDEAEQLALKGGKKQIQKLEARVRELEGEVESEQKRNAEAVKGLRKHERRVKELTYQTEEDRKNILRLQDLVDKLQAKVKSYKRQAEEAEEQSNTNLSKFRKLQHELEEAEERADIAESQVNKLRVKSREVHTKVISEE

Target ORF information:

RefSeq Version	XM_014113203.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris myosin, heavy chain 2, skeletal muscle, adult (MYH2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113203.2

ORF Insert Sequence:

ATGAGTTCAG ACCAGGAAAT GGCCATTTTC GGGGAGGCGG CTCCATACCT CCGGAAGTCT 
GAAAAGGAGC GCATTGAGGC CCAGAATAGG CCCTTTGATG CCAAGACGTC AGTCTTTGTG 
GCGGAGCCCA AAGAATCTTT TGTGAAAGGG ACTGTCCAGA GCAGAGAAGG AGGGAAAGTG 
ACAGTGAAGA CCGAAGCGGG AGCAACTCTG ACGGTGAAAG AGGACCAAGT CTTCCCCATG 
AACCCTCCCA AGTATGACAA GATCGAGGAC ATGGCCATGA TGACTCACCT GCACGAGCCT 
GCTGTGCTGT ACAACCTCAA AGAGCGTTAT GCAGCCTGGA TGATCTACAC CTATTCAGGC 
CTCTTCTGCG TCACTGTCAA CCCCTACAAG TGGCTGCCGG TGTACAACCC CGAGGTGGTG 
ACGGCCTACC GAGGCAAGAA GCGCCAGGAG GCCCCACCCC ACATCTTCTC CATCTCTGAC 
AATGCCTATC AGTTTATGCT GACTGACCGA GAGAATCAGT CAATCCTGAT CACTGGAGAA 
TCTGGTGCCG GGAAGACCGT GAACACCAAG CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTCACTGGAG AGAAGAAGAA GGAGGAAGCT ACTTCTGGCA AAATGCAGGG GACCCTTGAA 
GATCAGATCA TCAGTGCCAA CCCCCTACTG GAGGCCTTTG GCAACGCCAA GACTGTGAGG 
AATGACAACT CCTCCCGCTT TGGTAAATTC ATCAGAATTC ACTTTGGCAC TACAGGGAAG 
CTGGCTTCTG CTGATATTGA AACATATCTG TTAGAAAAGT CTAGAGTTAC TTTCCAGCTT 
AAGGCTGAAA GAAGCTATCA TATTTTTTAT CAGATCACAT CAAACAAGAA ACCAGAACTA 
ATTGAAATGC TTCTGATCAC CACCAACCCA TATGACTACC CATTTGTCAG TCAAGGGGAG 
ATCAGTGTGG CTAGCATTGA TGATCAAGAA GAGCTGATAG CAACAGATAG TGCTATTGAT 
ATTTTGGGCT TCACCAATGA AGAGAAAGTC TCCATCTACA AGCTCACGGG GGCCGTAATG 
CATTATGGTA ACTTGAAGTT CAAGCAGAAG CAGCGGGAGG AGCAGGCTGA GCCAGATGGC 
ACTGAAGTTG CTGACAAAGC AGCCTACCTC CAGAGCCTGA ACTCTGCTGA TCTGCTCAAA 
GCCCTCTGCT ACCCCAGGGT CAAGGTCGGC AATGAGTTTG TCACCAAAGG CCAGACTGTA 
GAGCAGGTAA CCAACGCAGT GGGCGCTCTG GCCAAGGCTG TCTATGAGAA GATGTTCCTG 
TGGATGGTCA CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCAGGCA GTACTTCATC 
GGGGTCTTGG ACATCGCTGG CTTTGAGATA TTTGATTTCA ACAGCCTGGA GCAGCTGTGT 
ATCAACTTCA CCAACGAGAA GCTCCAACAG TTCTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAGAAGGA GGGCATCGAG TGGACCTTCA TTGACTTCGG GATGGACCTG 
GCTGCCTGCA TCGAGCTCAT TGAGAAGCCC ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CCAAGGCCAC AGACACCTCC TTCAAGAACA AGCTGTATGA ACAGCATCTT 
GGAAAGTCTG CCAACTTCCA GAAGCCCAAG GTGGTCAAGG GCAAGGCCGA AGCACACTTC 
TCACTAATCC ACTACGCCGG CGTTGTGGAC TACAATATAA CTGGCTGGCT TGACAAGAAC 
AAGGACCCTC TCAATGAGAC TGTGGTTGGG CTGTACCAGA AGTCTTCTAT GAAAACTCTA 
GCTTACCTCT TCTCTGGGGC TCAAACTGCC GAAGCAGAGG CAAGTGGTGG TGCCAAGAAA 
GGTGGCAAGA AGAAGGGCTC TTCTTTCCAG ACGGTGTCTG CCCTTTTCAG GGAGAATCTG 
AACAAGCTGA TGACCAACCT CAGGAGTACC CATCCTCATT TTGTGCGCTG TATCATCCCC 
AATGAAACAA AAACTCCTGG GGCCATGGAG CATGAACTTG TCCTGCACCA GCTGCGGTGT 
AATGGTGTGC TGGAAGGGAT CCGCATCTGC CGGAAGGGGT TCCCAAGCAG GATCCTTTAT 
GCAGACTTCA AACAGAGATA CAAGGTATTA AATGCAAGTG CTATCCCAGA AGGACAGTTC 
ATTGACAGCA AGAAGGCTTC TGAGAAGCTC CTTGCATCCA TCGACATTGA CCACACCCAG 
TATAAATTTG GTCACACCAA GGTCTTTTTC AAAGCTGGTC TTCTGGGGCT CCTAGAGGAG 
ATGCGAGATG ACAAGCTAGC CCAGCTGATT ACCCGAACCC AGGCCAGGTG CAGAGGGTTC 
TTGGCAAGAG TGGAGTACCA GAAGATGGTG GAGAGAAGGG AGTCCATCTT CTGCATCCAG 
TATAACATCC GTGCCTTCAT GAATGTCAAG CACTGGCCCT GGATGAAGCT ATTCTTCAAG 
ATCAAACCCC TCCTCAAGAG CGCAGAGACC GAGAAGGAGA TGGCCACCAT GAAGGAAGAG 
TTTCAGAAAA CCAAAGATGA GCTTGCCAAA TCAGAGGCGA AAAGGAAGGA ACTGGAGGAA 
AAAATGGTCA CTCTCTTAAA AGAGAAAAAT GACCTGCAGC TCCAAGTTCA GGCTGAAGCT 
GAAGGCTTGG CTGATGCAGA GGAAAGATGT GACCAGCTGA TCAAAACCAA AATCCAGCTG 
GAGGCCAAGA TTAAGGAGGT GACTGAGAGA GCAGAGGATG AGGAGGAGAT CAATGCTGAG 
CTGACGGCCA AGAAGAGGAA ACTGGAAGAC GAGTGTTCAG AGCTCAAGAA AGATATTGAT 
GACCTTGAGC TGACCCTGGC CAAGGTTGAA AAGGAGAAGC ATGCCACAGA GAACAAGGTG 
AAAAACCTCA CTGAGGAGAT GGCAGGCCTG GATGAAACCA TTGCAAAGCT GACAAAGGAG 
AAGAAAGCCC TCCAAGAGGC CCACCAGCAG ACCCTGGATG ACCTACAGGC AGAAGAGGAC 
AAAGTCAACA CCCTGACCAA AGCTAAAATC AAGCTAGAGC AGCAAGTGGA TGACCTTGAA 
GGGTCCTTGG AGCAAGAAAA GAAACTTCGC ATGGATCTAG AAAGGGCTAA GAGGAAACTT 
GAAGGTGACC TCAAGTTGGC CCAAGAGTCC ATAATGGACA TTGAGAATGA AAAACAACAA 
CTTGATGAAA AGCTCAAAAA GAAAGAGTTT GAAATGAGCA ATCTGCAAAG CAAGATTGAA 
GACGAACAAG CACTTGGCAT TCAGCTGCAG AAGAAGATCA AAGAGCTGCA AGCCCGCATC 
GAGGAGCTGG AGGAGGAAAT CGAGGCAGAG CGCGCCTCCA GGGCCAAAGC AGAGAAGCAG 
CGCTCTGACC TCTCCCGGGA ACTGGAGGAG ATCAGTGAGC GCCTGGAAGA AGCTGGCGGG 
GCCACATCTG CCCAGATTGA GATGAACAAG AAGCGGGAGG CCGAGTTCCA GAAGATGCGC 
AGGGACCTGG AGGAGGCCAC CCTGCAGCAC GAAGCCACAG CAGCTACACT GAGAAAGAAG 
CACGCGGACA GCGTGGCCGA GCTCGGGGAG CAGATAGACA ACCTGCAGAG GGTCAAGCAG 
AAGCTGGAGA AGGAGAAGAG TGAGATGAAG ATGGAGATCG ATGATCTTGC CAGTAACGTA 
GAAACAGTTT CTAAAGCCAA GGGAAATCTA GAGAAAATGT GCCGCACTCT GGAGGACCAA 
GTGAGTGAGC TGAAATCAAA GGAGGAGGAG CAGCAGCGGC TGATCAATGA CCTGACCACC 
CAGAGGGGAC GCTTGCAGAC CGAATCTGGT GAATTTTCAC GACAGCTAGA TGAAAAAGAA 
GCTCTGGTGT CTCAGCTATC AAGGGGCAAA CTAGCATTTA CACAACAGAT TGAGGAATTA 
AAGAGGCAGC TGGAAGAAGA GATAAAGGCC AAGAACGCCC TGGCCCACGC CCTGCAGTCC 
TCCCGCCACG ACTGTGACCT GCTGCGGGAA CAGTATGAGG AGGAGCAGGA GTCCAAGGCC 
GAGCTGCAGA GGGCGCTGTC CAAGGCCAAC AGCGAGGTGG CCCAGTGGAG GACCAAATAT 
GAGACGGACG CCATCCAGCG CACGGAGGAG CTGGAGGAAG CAAAGAAGAA GCTGGCCCAG 
CGGTTGCAGG CGGCTGAGGA GCACGTAGAA GCTGTGAACG CCAAATGTGC TTCCCTGGAG 
AAGACAAAGC AACGGCTCCA GAATGAGGTG GAGGACCTCA TGCTGGACGT GGAGAGAACC 
AACGCAGCCT GCGCCGCCCT GGACAAGAAG CAGAGGAACT TCGACAAGAT CCTGGCAGAG 
TGGAAACAGA AGTATGAGGA AACCCATGCT GAACTCGAGG CCTCCCAGAA GGAGGCCCGC 
TCTCTCGGTA CTGAGCTGTT CAAGATGAAG AATGCCTACG AGGAGTCCTT GGATCAGCTA 
GAAACTTTGA AACGAGAGAA CAAAAACTTG CAGCAGGAGA TCTCTGACCT CACTGAGCAG 
ATTGCAGAGG GAGGGAAACG TATCCATGAA CTGGAAAAGA TAAAGAAACA AGTGGAACAA 
GAAAAGTCTG AAATTCAGGC TGCCTTGGAG GAAGCAGAGG CATCTCTTGA ACATGAAGAG 
GGAAAGATTC TGCGCATCCA GCTGGAGCTG AACCAAGTCA AGTCTGAGAT TGACAGGAAA 
ATCGCAGAAA AGGATGAGGA AATTGACCAG CTGAAGAGAA ACCACATCAG GGTTGTGGAG 
TCGATGCAGA CCATGCTGGA TGCCGAGATC AGGAGCAGGA ATGATGCCAT CAGGCTCAAG 
AAGAAGATGG AGGGAGACCT CAATGAGATG GAAATCCAGC TGAACCATGC CAACCGCATG 
GCTGCAGAGG CCCTGAGGAA CTACAGAAAT ACCCAAGGCA TCCTCAAAGA CACCCAGATC 
CACCTGGATG ACGCCCTCCG GGGCCAGGAG GACCTGAAGG AGCAGTTGGC CATGGTAGAG 
CGCAGAGCCA ACCTGCTTCA GGCTGAGATT GAGGAGCTGA GGGCCACTCT GGAGCAGACG 
GAGAGGAGCA GGAAAATCGC AGAACAGGAG CTCCTGGATG CCAGTGAGCG CGTCCAGCTC 
CTCCATACCC AGAACACTAG CCTGATCAAC ACCAAGAAGA AGCTGGAGAC AGACATTTCC 
CAGATCCAGG GAGAGATGGA GGACATTATC CAGGAAGCCC GCAATGCAGA GGAGAAGGCC 
AAGAAGGCCA TCACCGATGC GGCCATGATG GCCGAGGAGC TGAAGAAGGA GCAGGACACC 
AGCGCCCACC TGGAGCGGAT GAAGAAGAAC ATGGAGCAGA CGGTGAAGGA CCTGCAGCAT 
CGTCTGGATG AGGCTGAGCA GCTGGCCCTG AAGGGCGGGA AGAAACAGAT CCAGAAACTG 
GAGGCCAGGG TGCGTGAACT GGAAGGAGAG GTTGAGAGTG AGCAAAAGCG TAACGCGGAA 
GCTGTGAAAG GTCTGCGGAA ACACGAGAGG CGAGTAAAGG AACTCACTTA CCAGACGGAA 
GAAGATCGGA AAAATATTCT CAGACTTCAG GATTTGGTAG ATAAACTTCA AGCCAAAGTG 
AAATCTTACA AGAGACAAGC TGAGGAGGCT GAGGAACAAT CCAACACAAA TCTATCTAAA 
TTCCGCAAGC TCCAGCATGA GCTGGAGGAG GCTGAGGAGC GGGCTGACAT TGCCGAGTCC 
CAGGTCAACA AGTTGCGGGT GAAGAGCCGA GAGGTTCACA CGAAAGTCAT AAGTGAAGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH2 ( XM_005619532.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005619589.1 Canis lupus familiaris myosin, heavy chain 2, skeletal muscle, adult (MYH2), transcript variant X2, mRNA.

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CloneID	OCe05670
Clone ID Related Accession (Same CDS sequence)	NM_001076795.1 , XM_005619532.2 , XM_014113203.1 , XM_014113203.2 , XM_005619532.3
Accession Version	XM_005619532.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5823bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	myosin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005619532.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTCAG ACCAGGAAAT GGCCATTTTC GGGGAGGCGG CTCCATACCT CCGGAAGTCT 
GAAAAGGAGC GCATTGAGGC CCAGAATAGG CCCTTTGATG CCAAGACGTC AGTCTTTGTG 
GCGGAGCCCA AAGAATCTTT TGTGAAAGGG ACTGTCCAGA GCAGAGAAGG AGGGAAAGTG 
ACAGTGAAGA CCGAAGCGGG AGCAACTCTG ACGGTGAAAG AGGACCAAGT CTTCCCCATG 
AACCCTCCCA AGTATGACAA GATCGAGGAC ATGGCCATGA TGACTCACCT GCACGAGCCT 
GCTGTGCTGT ACAACCTCAA AGAGCGTTAT GCAGCCTGGA TGATCTACAC CTATTCAGGC 
CTCTTCTGCG TCACTGTCAA CCCCTACAAG TGGCTGCCGG TGTACAACCC CGAGGTGGTG 
ACGGCCTACC GAGGCAAGAA GCGCCAGGAG GCCCCACCCC ACATCTTCTC CATCTCTGAC 
AATGCCTATC AGTTTATGCT GACTGACCGA GAGAATCAGT CAATCCTGAT CACTGGAGAA 
TCTGGTGCCG GGAAGACCGT GAACACCAAG CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTCACTGGAG AGAAGAAGAA GGAGGAAGCT ACTTCTGGCA AAATGCAGGG GACCCTTGAA 
GATCAGATCA TCAGTGCCAA CCCCCTACTG GAGGCCTTTG GCAACGCCAA GACTGTGAGG 
AATGACAACT CCTCCCGCTT TGGTAAATTC ATCAGAATTC ACTTTGGCAC TACAGGGAAG 
CTGGCTTCTG CTGATATTGA AACATATCTG TTAGAAAAGT CTAGAGTTAC TTTCCAGCTT 
AAGGCTGAAA GAAGCTATCA TATTTTTTAT CAGATCACAT CAAACAAGAA ACCAGAACTA 
ATTGAAATGC TTCTGATCAC CACCAACCCA TATGACTACC CATTTGTCAG TCAAGGGGAG 
ATCAGTGTGG CTAGCATTGA TGATCAAGAA GAGCTGATAG CAACAGATAG TGCTATTGAT 
ATTTTGGGCT TCACCAATGA AGAGAAAGTC TCCATCTACA AGCTCACGGG GGCCGTAATG 
CATTATGGTA ACTTGAAGTT CAAGCAGAAG CAGCGGGAGG AGCAGGCTGA GCCAGATGGC 
ACTGAAGTTG CTGACAAAGC AGCCTACCTC CAGAGCCTGA ACTCTGCTGA TCTGCTCAAA 
GCCCTCTGCT ACCCCAGGGT CAAGGTCGGC AATGAGTTTG TCACCAAAGG CCAGACTGTA 
GAGCAGGTAA CCAACGCAGT GGGCGCTCTG GCCAAGGCTG TCTATGAGAA GATGTTCCTG 
TGGATGGTCA CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCAGGCA GTACTTCATC 
GGGGTCTTGG ACATCGCTGG CTTTGAGATA TTTGATTTCA ACAGCCTGGA GCAGCTGTGT 
ATCAACTTCA CCAACGAGAA GCTCCAACAG TTCTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAGAAGGA GGGCATCGAG TGGACCTTCA TTGACTTCGG GATGGACCTG 
GCTGCCTGCA TCGAGCTCAT TGAGAAGCCC ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CCAAGGCCAC AGACACCTCC TTCAAGAACA AGCTGTATGA ACAGCATCTT 
GGAAAGTCTG CCAACTTCCA GAAGCCCAAG GTGGTCAAGG GCAAGGCCGA AGCACACTTC 
TCACTAATCC ACTACGCCGG CGTTGTGGAC TACAATATAA CTGGCTGGCT TGACAAGAAC 
AAGGACCCTC TCAATGAGAC TGTGGTTGGG CTGTACCAGA AGTCTTCTAT GAAAACTCTA 
GCTTACCTCT TCTCTGGGGC TCAAACTGCC GAAGCAGAGG CAAGTGGTGG TGCCAAGAAA 
GGTGGCAAGA AGAAGGGCTC TTCTTTCCAG ACGGTGTCTG CCCTTTTCAG GGAGAATCTG 
AACAAGCTGA TGACCAACCT CAGGAGTACC CATCCTCATT TTGTGCGCTG TATCATCCCC 
AATGAAACAA AAACTCCTGG GGCCATGGAG CATGAACTTG TCCTGCACCA GCTGCGGTGT 
AATGGTGTGC TGGAAGGGAT CCGCATCTGC CGGAAGGGGT TCCCAAGCAG GATCCTTTAT 
GCAGACTTCA AACAGAGATA CAAGGTATTA AATGCAAGTG CTATCCCAGA AGGACAGTTC 
ATTGACAGCA AGAAGGCTTC TGAGAAGCTC CTTGCATCCA TCGACATTGA CCACACCCAG 
TATAAATTTG GTCACACCAA GGTCTTTTTC AAAGCTGGTC TTCTGGGGCT CCTAGAGGAG 
ATGCGAGATG ACAAGCTAGC CCAGCTGATT ACCCGAACCC AGGCCAGGTG CAGAGGGTTC 
TTGGCAAGAG TGGAGTACCA GAAGATGGTG GAGAGAAGGG AGTCCATCTT CTGCATCCAG 
TATAACATCC GTGCCTTCAT GAATGTCAAG CACTGGCCCT GGATGAAGCT ATTCTTCAAG 
ATCAAACCCC TCCTCAAGAG CGCAGAGACC GAGAAGGAGA TGGCCACCAT GAAGGAAGAG 
TTTCAGAAAA CCAAAGATGA GCTTGCCAAA TCAGAGGCGA AAAGGAAGGA ACTGGAGGAA 
AAAATGGTCA CTCTCTTAAA AGAGAAAAAT GACCTGCAGC TCCAAGTTCA GGCTGAAGCT 
GAAGGCTTGG CTGATGCAGA GGAAAGATGT GACCAGCTGA TCAAAACCAA AATCCAGCTG 
GAGGCCAAGA TTAAGGAGGT GACTGAGAGA GCAGAGGATG AGGAGGAGAT CAATGCTGAG 
CTGACGGCCA AGAAGAGGAA ACTGGAAGAC GAGTGTTCAG AGCTCAAGAA AGATATTGAT 
GACCTTGAGC TGACCCTGGC CAAGGTTGAA AAGGAGAAGC ATGCCACAGA GAACAAGGTG 
AAAAACCTCA CTGAGGAGAT GGCAGGCCTG GATGAAACCA TTGCAAAGCT GACAAAGGAG 
AAGAAAGCCC TCCAAGAGGC CCACCAGCAG ACCCTGGATG ACCTACAGGC AGAAGAGGAC 
AAAGTCAACA CCCTGACCAA AGCTAAAATC AAGCTAGAGC AGCAAGTGGA TGACCTTGAA 
GGGTCCTTGG AGCAAGAAAA GAAACTTCGC ATGGATCTAG AAAGGGCTAA GAGGAAACTT 
GAAGGTGACC TCAAGTTGGC CCAAGAGTCC ATAATGGACA TTGAGAATGA AAAACAACAA 
CTTGATGAAA AGCTCAAAAA GAAAGAGTTT GAAATGAGCA ATCTGCAAAG CAAGATTGAA 
GACGAACAAG CACTTGGCAT TCAGCTGCAG AAGAAGATCA AAGAGCTGCA AGCCCGCATC 
GAGGAGCTGG AGGAGGAAAT CGAGGCAGAG CGCGCCTCCA GGGCCAAAGC AGAGAAGCAG 
CGCTCTGACC TCTCCCGGGA ACTGGAGGAG ATCAGTGAGC GCCTGGAAGA AGCTGGCGGG 
GCCACATCTG CCCAGATTGA GATGAACAAG AAGCGGGAGG CCGAGTTCCA GAAGATGCGC 
AGGGACCTGG AGGAGGCCAC CCTGCAGCAC GAAGCCACAG CAGCTACACT GAGAAAGAAG 
CACGCGGACA GCGTGGCCGA GCTCGGGGAG CAGATAGACA ACCTGCAGAG GGTCAAGCAG 
AAGCTGGAGA AGGAGAAGAG TGAGATGAAG ATGGAGATCG ATGATCTTGC CAGTAACGTA 
GAAACAGTTT CTAAAGCCAA GGGAAATCTA GAGAAAATGT GCCGCACTCT GGAGGACCAA 
GTGAGTGAGC TGAAATCAAA GGAGGAGGAG CAGCAGCGGC TGATCAATGA CCTGACCACC 
CAGAGGGGAC GCTTGCAGAC CGAATCTGGT GAATTTTCAC GACAGCTAGA TGAAAAAGAA 
GCTCTGGTGT CTCAGCTATC AAGGGGCAAA CTAGCATTTA CACAACAGAT TGAGGAATTA 
AAGAGGCAGC TGGAAGAAGA GATAAAGGCC AAGAACGCCC TGGCCCACGC CCTGCAGTCC 
TCCCGCCACG ACTGTGACCT GCTGCGGGAA CAGTATGAGG AGGAGCAGGA GTCCAAGGCC 
GAGCTGCAGA GGGCGCTGTC CAAGGCCAAC AGCGAGGTGG CCCAGTGGAG GACCAAATAT 
GAGACGGACG CCATCCAGCG CACGGAGGAG CTGGAGGAAG CAAAGAAGAA GCTGGCCCAG 
CGGTTGCAGG CGGCTGAGGA GCACGTAGAA GCTGTGAACG CCAAATGTGC TTCCCTGGAG 
AAGACAAAGC AACGGCTCCA GAATGAGGTG GAGGACCTCA TGCTGGACGT GGAGAGAACC 
AACGCAGCCT GCGCCGCCCT GGACAAGAAG CAGAGGAACT TCGACAAGAT CCTGGCAGAG 
TGGAAACAGA AGTATGAGGA AACCCATGCT GAACTCGAGG CCTCCCAGAA GGAGGCCCGC 
TCTCTCGGTA CTGAGCTGTT CAAGATGAAG AATGCCTACG AGGAGTCCTT GGATCAGCTA 
GAAACTTTGA AACGAGAGAA CAAAAACTTG CAGCAGGAGA TCTCTGACCT CACTGAGCAG 
ATTGCAGAGG GAGGGAAACG TATCCATGAA CTGGAAAAGA TAAAGAAACA AGTGGAACAA 
GAAAAGTCTG AAATTCAGGC TGCCTTGGAG GAAGCAGAGG CATCTCTTGA ACATGAAGAG 
GGAAAGATTC TGCGCATCCA GCTGGAGCTG AACCAAGTCA AGTCTGAGAT TGACAGGAAA 
ATCGCAGAAA AGGATGAGGA AATTGACCAG CTGAAGAGAA ACCACATCAG GGTTGTGGAG 
TCGATGCAGA CCATGCTGGA TGCCGAGATC AGGAGCAGGA ATGATGCCAT CAGGCTCAAG 
AAGAAGATGG AGGGAGACCT CAATGAGATG GAAATCCAGC TGAACCATGC CAACCGCATG 
GCTGCAGAGG CCCTGAGGAA CTACAGAAAT ACCCAAGGCA TCCTCAAAGA CACCCAGATC 
CACCTGGATG ACGCCCTCCG GGGCCAGGAG GACCTGAAGG AGCAGTTGGC CATGGTAGAG 
CGCAGAGCCA ACCTGCTTCA GGCTGAGATT GAGGAGCTGA GGGCCACTCT GGAGCAGACG 
GAGAGGAGCA GGAAAATCGC AGAACAGGAG CTCCTGGATG CCAGTGAGCG CGTCCAGCTC 
CTCCATACCC AGAACACTAG CCTGATCAAC ACCAAGAAGA AGCTGGAGAC AGACATTTCC 
CAGATCCAGG GAGAGATGGA GGACATTATC CAGGAAGCCC GCAATGCAGA GGAGAAGGCC 
AAGAAGGCCA TCACCGATGC GGCCATGATG GCCGAGGAGC TGAAGAAGGA GCAGGACACC 
AGCGCCCACC TGGAGCGGAT GAAGAAGAAC ATGGAGCAGA CGGTGAAGGA CCTGCAGCAT 
CGTCTGGATG AGGCTGAGCA GCTGGCCCTG AAGGGCGGGA AGAAACAGAT CCAGAAACTG 
GAGGCCAGGG TGCGTGAACT GGAAGGAGAG GTTGAGAGTG AGCAAAAGCG TAACGCGGAA 
GCTGTGAAAG GTCTGCGGAA ACACGAGAGG CGAGTAAAGG AACTCACTTA CCAGACGGAA 
GAAGATCGGA AAAATATTCT CAGACTTCAG GATTTGGTAG ATAAACTTCA AGCCAAAGTG 
AAATCTTACA AGAGACAAGC TGAGGAGGCT GAGGAACAAT CCAACACAAA TCTATCTAAA 
TTCCGCAAGC TCCAGCATGA GCTGGAGGAG GCTGAGGAGC GGGCTGACAT TGCCGAGTCC 
CAGGTCAACA AGTTGCGGGT GAAGAGCCGA GAGGTTCACA CGAAAGTCAT AAGTGAAGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005619589.1
CDS	158..5980
Translation	MSSDQEMAIFGEAAPYLRKSEKERIEAQNRPFDAKTSVFVAEPKESFVKGTVQSREGGKVTVKTEAGATLTVKEDQVFPMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNPEVVTAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEATSGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSYHIFYQITSNKKPELIEMLLITTNPYDYPFVSQGEISVASIDDQEELIATDSAIDILGFTNEEKVSIYKLTGAVMHYGNLKFKQKQREEQAEPDGTEVADKAAYLQSLNSADLLKALCYPRVKVGNEFVTKGQTVEQVTNAVGALAKAVYEKMFLWMVTRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYEQHLGKSANFQKPKVVKGKAEAHFSLIHYAGVVDYNITGWLDKNKDPLNETVVGLYQKSSMKTLAYLFSGAQTAEAEASGGAKKGGKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQFIDSKKASEKLLASIDIDHTQYKFGHTKVFFKAGLLGLLEEMRDDKLAQLITRTQARCRGFLARVEYQKMVERRESIFCIQYNIRAFMNVKHWPWMKLFFKIKPLLKSAETEKEMATMKEEFQKTKDELAKSEAKRKELEEKMVTLLKEKNDLQLQVQAEAEGLADAEERCDQLIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDETIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKIKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAQESIMDIENEKQQLDEKLKKKEFEMSNLQSKIEDEQALGIQLQKKIKELQARIEELEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEEATLQHEATAATLRKKHADSVAELGEQIDNLQRVKQKLEKEKSEMKMEIDDLASNVETVSKAKGNLEKMCRTLEDQVSELKSKEEEQQRLINDLTTQRGRLQTESGEFSRQLDEKEALVSQLSRGKLAFTQQIEELKRQLEEEIKAKNALAHALQSSRHDCDLLREQYEEEQESKAELQRALSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQAAEEHVEAVNAKCASLEKTKQRLQNEVEDLMLDVERTNAACAALDKKQRNFDKILAEWKQKYEETHAELEASQKEARSLGTELFKMKNAYEESLDQLETLKRENKNLQQEISDLTEQIAEGGKRIHELEKIKKQVEQEKSEIQAALEEAEASLEHEEGKILRIQLELNQVKSEIDRKIAEKDEEIDQLKRNHIRVVESMQTMLDAEIRSRNDAIRLKKKMEGDLNEMEIQLNHANRMAAEALRNYRNTQGILKDTQIHLDDALRGQEDLKEQLAMVERRANLLQAEIEELRATLEQTERSRKIAEQELLDASERVQLLHTQNTSLINTKKKLETDISQIQGEMEDIIQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTVKDLQHRLDEAEQLALKGGKKQIQKLEARVRELEGEVESEQKRNAEAVKGLRKHERRVKELTYQTEEDRKNILRLQDLVDKLQAKVKSYKRQAEEAEEQSNTNLSKFRKLQHELEEAEERADIAESQVNKLRVKSREVHTKVISEE

Target ORF information:

RefSeq Version	XM_005619532.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris myosin, heavy chain 2, skeletal muscle, adult (MYH2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005619532.3

ORF Insert Sequence:

ATGAGTTCAG ACCAGGAAAT GGCCATTTTC GGGGAGGCGG CTCCATACCT CCGGAAGTCT 
GAAAAGGAGC GCATTGAGGC CCAGAATAGG CCCTTTGATG CCAAGACGTC AGTCTTTGTG 
GCGGAGCCCA AAGAATCTTT TGTGAAAGGG ACTGTCCAGA GCAGAGAAGG AGGGAAAGTG 
ACAGTGAAGA CCGAAGCGGG AGCAACTCTG ACGGTGAAAG AGGACCAAGT CTTCCCCATG 
AACCCTCCCA AGTATGACAA GATCGAGGAC ATGGCCATGA TGACTCACCT GCACGAGCCT 
GCTGTGCTGT ACAACCTCAA AGAGCGTTAT GCAGCCTGGA TGATCTACAC CTATTCAGGC 
CTCTTCTGCG TCACTGTCAA CCCCTACAAG TGGCTGCCGG TGTACAACCC CGAGGTGGTG 
ACGGCCTACC GAGGCAAGAA GCGCCAGGAG GCCCCACCCC ACATCTTCTC CATCTCTGAC 
AATGCCTATC AGTTTATGCT GACTGACCGA GAGAATCAGT CAATCCTGAT CACTGGAGAA 
TCTGGTGCCG GGAAGACCGT GAACACCAAG CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTCACTGGAG AGAAGAAGAA GGAGGAAGCT ACTTCTGGCA AAATGCAGGG GACCCTTGAA 
GATCAGATCA TCAGTGCCAA CCCCCTACTG GAGGCCTTTG GCAACGCCAA GACTGTGAGG 
AATGACAACT CCTCCCGCTT TGGTAAATTC ATCAGAATTC ACTTTGGCAC TACAGGGAAG 
CTGGCTTCTG CTGATATTGA AACATATCTG TTAGAAAAGT CTAGAGTTAC TTTCCAGCTT 
AAGGCTGAAA GAAGCTATCA TATTTTTTAT CAGATCACAT CAAACAAGAA ACCAGAACTA 
ATTGAAATGC TTCTGATCAC CACCAACCCA TATGACTACC CATTTGTCAG TCAAGGGGAG 
ATCAGTGTGG CTAGCATTGA TGATCAAGAA GAGCTGATAG CAACAGATAG TGCTATTGAT 
ATTTTGGGCT TCACCAATGA AGAGAAAGTC TCCATCTACA AGCTCACGGG GGCCGTAATG 
CATTATGGTA ACTTGAAGTT CAAGCAGAAG CAGCGGGAGG AGCAGGCTGA GCCAGATGGC 
ACTGAAGTTG CTGACAAAGC AGCCTACCTC CAGAGCCTGA ACTCTGCTGA TCTGCTCAAA 
GCCCTCTGCT ACCCCAGGGT CAAGGTCGGC AATGAGTTTG TCACCAAAGG CCAGACTGTA 
GAGCAGGTAA CCAACGCAGT GGGCGCTCTG GCCAAGGCTG TCTATGAGAA GATGTTCCTG 
TGGATGGTCA CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCAGGCA GTACTTCATC 
GGGGTCTTGG ACATCGCTGG CTTTGAGATA TTTGATTTCA ACAGCCTGGA GCAGCTGTGT 
ATCAACTTCA CCAACGAGAA GCTCCAACAG TTCTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAGAAGGA GGGCATCGAG TGGACCTTCA TTGACTTCGG GATGGACCTG 
GCTGCCTGCA TCGAGCTCAT TGAGAAGCCC ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CCAAGGCCAC AGACACCTCC TTCAAGAACA AGCTGTATGA ACAGCATCTT 
GGAAAGTCTG CCAACTTCCA GAAGCCCAAG GTGGTCAAGG GCAAGGCCGA AGCACACTTC 
TCACTAATCC ACTACGCCGG CGTTGTGGAC TACAATATAA CTGGCTGGCT TGACAAGAAC 
AAGGACCCTC TCAATGAGAC TGTGGTTGGG CTGTACCAGA AGTCTTCTAT GAAAACTCTA 
GCTTACCTCT TCTCTGGGGC TCAAACTGCC GAAGCAGAGG CAAGTGGTGG TGCCAAGAAA 
GGTGGCAAGA AGAAGGGCTC TTCTTTCCAG ACGGTGTCTG CCCTTTTCAG GGAGAATCTG 
AACAAGCTGA TGACCAACCT CAGGAGTACC CATCCTCATT TTGTGCGCTG TATCATCCCC 
AATGAAACAA AAACTCCTGG GGCCATGGAG CATGAACTTG TCCTGCACCA GCTGCGGTGT 
AATGGTGTGC TGGAAGGGAT CCGCATCTGC CGGAAGGGGT TCCCAAGCAG GATCCTTTAT 
GCAGACTTCA AACAGAGATA CAAGGTATTA AATGCAAGTG CTATCCCAGA AGGACAGTTC 
ATTGACAGCA AGAAGGCTTC TGAGAAGCTC CTTGCATCCA TCGACATTGA CCACACCCAG 
TATAAATTTG GTCACACCAA GGTCTTTTTC AAAGCTGGTC TTCTGGGGCT CCTAGAGGAG 
ATGCGAGATG ACAAGCTAGC CCAGCTGATT ACCCGAACCC AGGCCAGGTG CAGAGGGTTC 
TTGGCAAGAG TGGAGTACCA GAAGATGGTG GAGAGAAGGG AGTCCATCTT CTGCATCCAG 
TATAACATCC GTGCCTTCAT GAATGTCAAG CACTGGCCCT GGATGAAGCT ATTCTTCAAG 
ATCAAACCCC TCCTCAAGAG CGCAGAGACC GAGAAGGAGA TGGCCACCAT GAAGGAAGAG 
TTTCAGAAAA CCAAAGATGA GCTTGCCAAA TCAGAGGCGA AAAGGAAGGA ACTGGAGGAA 
AAAATGGTCA CTCTCTTAAA AGAGAAAAAT GACCTGCAGC TCCAAGTTCA GGCTGAAGCT 
GAAGGCTTGG CTGATGCAGA GGAAAGATGT GACCAGCTGA TCAAAACCAA AATCCAGCTG 
GAGGCCAAGA TTAAGGAGGT GACTGAGAGA GCAGAGGATG AGGAGGAGAT CAATGCTGAG 
CTGACGGCCA AGAAGAGGAA ACTGGAAGAC GAGTGTTCAG AGCTCAAGAA AGATATTGAT 
GACCTTGAGC TGACCCTGGC CAAGGTTGAA AAGGAGAAGC ATGCCACAGA GAACAAGGTG 
AAAAACCTCA CTGAGGAGAT GGCAGGCCTG GATGAAACCA TTGCAAAGCT GACAAAGGAG 
AAGAAAGCCC TCCAAGAGGC CCACCAGCAG ACCCTGGATG ACCTACAGGC AGAAGAGGAC 
AAAGTCAACA CCCTGACCAA AGCTAAAATC AAGCTAGAGC AGCAAGTGGA TGACCTTGAA 
GGGTCCTTGG AGCAAGAAAA GAAACTTCGC ATGGATCTAG AAAGGGCTAA GAGGAAACTT 
GAAGGTGACC TCAAGTTGGC CCAAGAGTCC ATAATGGACA TTGAGAATGA AAAACAACAA 
CTTGATGAAA AGCTCAAAAA GAAAGAGTTT GAAATGAGCA ATCTGCAAAG CAAGATTGAA 
GACGAACAAG CACTTGGCAT TCAGCTGCAG AAGAAGATCA AAGAGCTGCA AGCCCGCATC 
GAGGAGCTGG AGGAGGAAAT CGAGGCAGAG CGCGCCTCCA GGGCCAAAGC AGAGAAGCAG 
CGCTCTGACC TCTCCCGGGA ACTGGAGGAG ATCAGTGAGC GCCTGGAAGA AGCTGGCGGG 
GCCACATCTG CCCAGATTGA GATGAACAAG AAGCGGGAGG CCGAGTTCCA GAAGATGCGC 
AGGGACCTGG AGGAGGCCAC CCTGCAGCAC GAAGCCACAG CAGCTACACT GAGAAAGAAG 
CACGCGGACA GCGTGGCCGA GCTCGGGGAG CAGATAGACA ACCTGCAGAG GGTCAAGCAG 
AAGCTGGAGA AGGAGAAGAG TGAGATGAAG ATGGAGATCG ATGATCTTGC CAGTAACGTA 
GAAACAGTTT CTAAAGCCAA GGGAAATCTA GAGAAAATGT GCCGCACTCT GGAGGACCAA 
GTGAGTGAGC TGAAATCAAA GGAGGAGGAG CAGCAGCGGC TGATCAATGA CCTGACCACC 
CAGAGGGGAC GCTTGCAGAC CGAATCTGGT GAATTTTCAC GACAGCTAGA TGAAAAAGAA 
GCTCTGGTGT CTCAGCTATC AAGGGGCAAA CTAGCATTTA CACAACAGAT TGAGGAATTA 
AAGAGGCAGC TGGAAGAAGA GATAAAGGCC AAGAACGCCC TGGCCCACGC CCTGCAGTCC 
TCCCGCCACG ACTGTGACCT GCTGCGGGAA CAGTATGAGG AGGAGCAGGA GTCCAAGGCC 
GAGCTGCAGA GGGCGCTGTC CAAGGCCAAC AGCGAGGTGG CCCAGTGGAG GACCAAATAT 
GAGACGGACG CCATCCAGCG CACGGAGGAG CTGGAGGAAG CAAAGAAGAA GCTGGCCCAG 
CGGTTGCAGG CGGCTGAGGA GCACGTAGAA GCTGTGAACG CCAAATGTGC TTCCCTGGAG 
AAGACAAAGC AACGGCTCCA GAATGAGGTG GAGGACCTCA TGCTGGACGT GGAGAGAACC 
AACGCAGCCT GCGCCGCCCT GGACAAGAAG CAGAGGAACT TCGACAAGAT CCTGGCAGAG 
TGGAAACAGA AGTATGAGGA AACCCATGCT GAACTCGAGG CCTCCCAGAA GGAGGCCCGC 
TCTCTCGGTA CTGAGCTGTT CAAGATGAAG AATGCCTACG AGGAGTCCTT GGATCAGCTA 
GAAACTTTGA AACGAGAGAA CAAAAACTTG CAGCAGGAGA TCTCTGACCT CACTGAGCAG 
ATTGCAGAGG GAGGGAAACG TATCCATGAA CTGGAAAAGA TAAAGAAACA AGTGGAACAA 
GAAAAGTCTG AAATTCAGGC TGCCTTGGAG GAAGCAGAGG CATCTCTTGA ACATGAAGAG 
GGAAAGATTC TGCGCATCCA GCTGGAGCTG AACCAAGTCA AGTCTGAGAT TGACAGGAAA 
ATCGCAGAAA AGGATGAGGA AATTGACCAG CTGAAGAGAA ACCACATCAG GGTTGTGGAG 
TCGATGCAGA CCATGCTGGA TGCCGAGATC AGGAGCAGGA ATGATGCCAT CAGGCTCAAG 
AAGAAGATGG AGGGAGACCT CAATGAGATG GAAATCCAGC TGAACCATGC CAACCGCATG 
GCTGCAGAGG CCCTGAGGAA CTACAGAAAT ACCCAAGGCA TCCTCAAAGA CACCCAGATC 
CACCTGGATG ACGCCCTCCG GGGCCAGGAG GACCTGAAGG AGCAGTTGGC CATGGTAGAG 
CGCAGAGCCA ACCTGCTTCA GGCTGAGATT GAGGAGCTGA GGGCCACTCT GGAGCAGACG 
GAGAGGAGCA GGAAAATCGC AGAACAGGAG CTCCTGGATG CCAGTGAGCG CGTCCAGCTC 
CTCCATACCC AGAACACTAG CCTGATCAAC ACCAAGAAGA AGCTGGAGAC AGACATTTCC 
CAGATCCAGG GAGAGATGGA GGACATTATC CAGGAAGCCC GCAATGCAGA GGAGAAGGCC 
AAGAAGGCCA TCACCGATGC GGCCATGATG GCCGAGGAGC TGAAGAAGGA GCAGGACACC 
AGCGCCCACC TGGAGCGGAT GAAGAAGAAC ATGGAGCAGA CGGTGAAGGA CCTGCAGCAT 
CGTCTGGATG AGGCTGAGCA GCTGGCCCTG AAGGGCGGGA AGAAACAGAT CCAGAAACTG 
GAGGCCAGGG TGCGTGAACT GGAAGGAGAG GTTGAGAGTG AGCAAAAGCG TAACGCGGAA 
GCTGTGAAAG GTCTGCGGAA ACACGAGAGG CGAGTAAAGG AACTCACTTA CCAGACGGAA 
GAAGATCGGA AAAATATTCT CAGACTTCAG GATTTGGTAG ATAAACTTCA AGCCAAAGTG 
AAATCTTACA AGAGACAAGC TGAGGAGGCT GAGGAACAAT CCAACACAAA TCTATCTAAA 
TTCCGCAAGC TCCAGCATGA GCTGGAGGAG GCTGAGGAGC GGGCTGACAT TGCCGAGTCC 
CAGGTCAACA AGTTGCGGGT GAAGAGCCGA GAGGTTCACA CGAAAGTCAT AAGTGAAGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The E3 ligase Ubr3 regulates Usher syndrome and MYH9 disorder proteins in the auditory organs of Drosophila and mammals.
eLife5(2016 06)
Li T,Giagtzoglou N,Eberl DF,Jaiswal SN,Cai T,Godt D,Groves AK,Bellen HJ

Regulation of myosin activation during cell-cell contact formation by Par3-Lgl antagonism: entosis without matrix detachment.
Molecular biology of the cell23(11)2076-91(2012 Jun)
Wan Q,Liu J,Zheng Z,Zhu H,Chu X,Dong Z,Huang S,Du Q

The N-terminal domain of the type 1 Ins(1,4,5)P3 receptor stably expressed in MDCK cells interacts with myosin IIA and alters epithelial cell morphology.
Journal of cell science123(Pt 9)1449-59(2010 May)
Hours MC,Mery L

Myosin IIA dependent retrograde flow drives 3D cell migration.
Biophysical journal98(8)L29-31(2010 Apr)
Shih W,Yamada S

Identification of myosin heavy chain I, IIa and IIx in canine skeletal muscles by an electrophoretic and immunoblotting study.
Cells, tissues, organs180(2)106-16(2005)
Smerdu V,Strbenc M,Meznaric-Petrusa M,Fazarinc G

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MYH2 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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