DMD cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	DMD
Entrez Gene ID	606758
Full Name	dystrophin
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005641029.3	XP_005641086.1	dystrophin isoform X1
XM_005641030.3	XP_005641087.1	dystrophin isoform X5
XM_005641037.2	XP_005641094.1	dystrophin isoform X13
XM_005641038.1	XP_005641095.1	dystrophin isoform X13
XM_014111351.1	XP_013966826.1	dystrophin isoform X15
XM_014111344.1	XP_013966819.1	dystrophin isoform X4
XM_014111347.1	XP_013966822.1	dystrophin isoform X8
XM_005641029.2	XP_005641086.1	dystrophin isoform X1
XM_014111346.1	XP_013966821.1	dystrophin isoform X7
XM_014111349.1	XP_013966824.1	dystrophin isoform X11
XM_014111345.2	XP_013966820.1	dystrophin isoform X6
XM_014111350.2	XP_013966825.1	dystrophin isoform X15
XM_014111345.1	XP_013966820.1	dystrophin isoform X6
XM_014111343.2	XP_013966818.1	dystrophin isoform X3
XM_014111344.2	XP_013966819.1	dystrophin isoform X4
XM_014111347.2	XP_013966822.1	dystrophin isoform X8
XM_005641038.2	XP_005641095.1	dystrophin isoform X14
XM_005641037.1	XP_005641094.1	dystrophin isoform X12
XM_014111348.1	XP_013966823.1	dystrophin isoform X10
XM_005641032.2	XP_005641089.1	dystrophin isoform X9
XM_022415519.1	XP_022271227.1	dystrophin isoform X9
XM_014111349.2	XP_013966824.1	dystrophin isoform X10
XM_014111350.1	XP_013966825.1	dystrophin isoform X14
XM_014111342.1	XP_013966817.1	dystrophin isoform X2
XM_014111343.1	XP_013966818.1	dystrophin isoform X3
XM_005641030.2	XP_005641087.1	dystrophin isoform X5
XM_014111342.2	XP_013966817.1	dystrophin isoform X2
XM_014111346.2	XP_013966821.1	dystrophin isoform X7
XM_022415520.1	XP_022271228.1	dystrophin isoform X11
XM_022415521.1	XP_022271229.1	dystrophin isoform X12
XM_014111351.2	XP_013966826.1	dystrophin isoform X16
NM_001003343.1	NP_001003343.1	dystrophin

Pathways from BioSystems

Homologies

Homo sapiens (human)	DMD	NP_003997.1
Pan troglodytes (chimpanzee)	LOC465559	XP_003317462.2
Mus musculus (house mouse)	Dmd	NP_031894.1
Gallus gallus (chicken)	DMD	NP_990630.1
Canis lupus familiaris (dog)	DMD	NP_001003343.1
Drosophila melanogaster (fruit fly)	Dys	NP_001262730.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010261	XP_319450.4

Gene Ontology
Bibliography

Related articles in PubMed

Whole genome sequencing reveals a 7 base-pair deletion in DMD exon 42 in a dog with muscular dystrophy.
Nghiem PP, Bello L, Balog-Alvarez C, L?pez SM, Bettis A, Barnett H, Hernandez B, Schatzberg SJ, Piercy RJ, Kornegay JN
Mammalian genome : official journal of the International Mammalian Genome Society28(3-4)106-113(2017 Apr)

Dystrophin-deficient dogs with reduced myostatin have unequal muscle growth and greater joint contractures.
Kornegay JN, Bogan DJ, Bogan JR, Dow JL, Wang J, Fan Z, Liu N, Warsing LC, Grange RW, Ahn M, Balog-Alvarez CJ, Cotten SW, Willis MS, Brinkmeyer-Langford C, Zhu H, Palandra J, Morris CA, Styner MA, Wagner KR
Skeletal muscle614(2016)

Muscular dystrophy in the Japanese Spitz: an inversion disrupts the DMD and RPGR genes.
Atencia-Fernandez S, Shiel RE, Mooney CT, Nolan CM
Animal genetics46(2)175-84(2015 Apr)

Role of dystrophin in airway smooth muscle phenotype, contraction and lung function.
Sharma P, Basu S, Mitchell RW, Stelmack GL, Anderson JE, Halayko AJ
PloS one9(7)e102737(2014)

A comparative study of N-glycolylneuraminic acid (Neu5Gc) and cytotoxic T cell (CT) carbohydrate expression in normal and dystrophin-deficient dog and human skeletal muscle.
Martin PT, Golden B, Okerblom J, Camboni M, Chandrasekharan K, Xu R, Varki A, Flanigan KM, Kornegay JN
PloS one9(2)e88226(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The deletion was confirmed with conventional PCR and Sanger sequencing. This mutation is in a secondary DMD gene hotspot area distinct from the one identified earlier at the 5' donor splice site of intron 50 in the CKCS breed.
Title: Whole genome sequencing reveals a 7 base-pair deletion in DMD exon 42 in a dog with muscular dystrophy.

Partial myostatin loss may exaggerate selective muscle hypertrophy or atrophy/hypoplasia in dystrophin-deficient dogs and worsen contractures.
Title: Dystrophin-deficient dogs with reduced myostatin have unequal muscle growth and greater joint contractures.

a chromosome inversion disrupting the DMD and RPGR genes is the causative mutation for X-linked muscular dystrophy in the Japanese Spitz breed
Title: Muscular dystrophy in the Japanese Spitz: an inversion disrupts the DMD and RPGR genes.

Data indicate that dystrophin affects caveolar integrity and airway smooth muscle contraction.
Title: Role of dystrophin in airway smooth muscle phenotype, contraction and lung function.

These data are consistent with the notion that altered N-glycolylneuraminic acid and cytotoxic T cell carbohydrate expression may modify severity of muscular dystrophy resulting from dystrophin deficiency in dogs and humans.
Title: A comparative study of N-glycolylneuraminic acid (Neu5Gc) and cytotoxic T cell (CT) carbohydrate expression in normal and dystrophin-deficient dog and human skeletal muscle.

The following DMD gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DMD cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe31981	XM_005641037.2 Latest version!	Canis lupus familiaris dystrophin (DMD), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OCe31982	XM_005641038.2 Latest version!	Canis lupus familiaris dystrophin (DMD), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OCe285080	XM_022415521.1 Latest version!	Canis lupus familiaris dystrophin (DMD), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OCe45421	XM_014111350.2 Latest version!	Canis lupus familiaris dystrophin (DMD), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OCe45422	XM_014111351.2 Latest version!	Canis lupus familiaris dystrophin (DMD), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OCe285079	XM_022415520.1 Latest version!	Canis lupus familiaris dystrophin (DMD), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe00364	NM_001003343.1 Latest version!	Canis lupus familiaris dystrophin (DMD), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OCe285078	XM_022415519.1 Latest version!	Canis lupus familiaris dystrophin (DMD), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OCe31973	XM_005641029.3 Latest version!	Canis lupus familiaris dystrophin (DMD), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OCe31974	XM_005641030.3 Latest version!	Canis lupus familiaris dystrophin (DMD), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OCe31976	XM_005641032.2 Latest version!	Canis lupus familiaris dystrophin (DMD), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OCe45413	XM_014111342.2 Latest version!	Canis lupus familiaris dystrophin (DMD), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OCe45414	XM_014111343.2 Latest version!	Canis lupus familiaris dystrophin (DMD), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OCe45415	XM_014111344.2 Latest version!	Canis lupus familiaris dystrophin (DMD), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OCe45416	XM_014111345.2 Latest version!	Canis lupus familiaris dystrophin (DMD), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OCe45417	XM_014111346.2 Latest version!	Canis lupus familiaris dystrophin (DMD), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OCe45418	XM_014111347.2 Latest version!	Canis lupus familiaris dystrophin (DMD), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OCe45419	XM_014111348.1 Latest version!	Canis lupus familiaris dystrophin (DMD), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OCe45420	XM_014111349.2 Latest version!	Canis lupus familiaris dystrophin (DMD), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OCe31973	XM_005641029.2	Canis lupus familiaris dystrophin (DMD), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OCe45416	XM_014111345.1	Canis lupus familiaris dystrophin (DMD), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OCe45415	XM_014111344.1	Canis lupus familiaris dystrophin (DMD), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OCe45418	XM_014111347.1	Canis lupus familiaris dystrophin (DMD), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OCe31982	XM_005641038.1	Canis lupus familiaris dystrophin (DMD), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OCe31981	XM_005641037.1	Canis lupus familiaris dystrophin (DMD), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OCe45420	XM_014111349.1	Canis lupus familiaris dystrophin (DMD), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OCe45421	XM_014111350.1	Canis lupus familiaris dystrophin (DMD), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OCe45414	XM_014111343.1	Canis lupus familiaris dystrophin (DMD), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OCe31974	XM_005641030.2	Canis lupus familiaris dystrophin (DMD), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OCe45413	XM_014111342.1	Canis lupus familiaris dystrophin (DMD), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OCe45417	XM_014111346.1	Canis lupus familiaris dystrophin (DMD), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OCe45422	XM_014111351.1	Canis lupus familiaris dystrophin (DMD), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DMD ( XM_005641037.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005641094.1 Canis lupus familiaris dystrophin (DMD), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OCe31981
Clone ID Related Accession (Same CDS sequence)	XM_005641037.2 , XM_005641037.1
Accession Version	XM_005641037.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1908bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	dystrophin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005641037.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGAGC AGCTCAAAGG CCACGAGACC CAAACAACTT GCTGGGACCA TCCCAAAATG 
ACAGAGCTCT ACCAGTCTTT AGCTGACCTG AATAATGTCA GGTTCTCAGC TTACAGGACT 
GCCATGAAAC TCCGAAGACT GCAGAAGGCC CTTTGCTTGG ATCTCTTGAG CCTATCGGCT 
GCATGCGATG CCTTGGACCA GCACAACCTC AAGCAAAATG ACCAGCCCAT GGATATCCTG 
CAGGTCATTA ACTGTCTGAC CACTATTTAT GATCGCCTAG AGCAAGAGCA CAACAATCTG 
GTCAACGTCC CTCTCTGCGT GGATATGTGT CTCAATTGGC TGCTGAATGT TTATGACACG 
GGACGAACGG GGAGGATCCG GGTCCTGTCT TTTAAAACTG GCATCATTTC TCTGTGTAAA 
GCCCATTTGG AAGACAAGTA CAGATACCTC TTCAAGCAAG TGGCAAGTTC GACAGGATTT 
TGTGACCAGC GCAGGCTGGG CCTCCTCCTG CATGACTCTA TCCAGATCCC AAGACAGTTG 
GGTGAAGTCG CATCGTTCGG GGGCAGTAAC ATTGAGCCGA GTGTCAGGAG CTGCTTCCAG 
TTTGCTAATA ATAAGCCTGA GATCGAAGCG GCCCTCTTCC TAGACTGGAT GCGCCTGGAG 
CCCCAGTCCA TGGTGTGGCT GCCTGTCCTG CACCGAGTGG CTGCCGCGGA AACTGCCAAG 
CACCAGGCCA AGTGCAACAT CTGCAAGGAG TGTCCCATCA TCGGATTCAG GTACAGGAGT 
CTAAAGCACT TTAATTATGA CATCTGCCAA AGTTGCTTTT TTTCTGGTCG AGTTGCAAAA 
GGCCATAAAA TGCACTATCC CATGGTGGAA TACTGCACTC CGACTACATC GGGAGAAGAT 
GTCCGTGACT TTGCCAAGGT ACTAAAAAAC AAATTTCGAA CCAAAAGGTA TTTTGCGAAG 
CATCCCCGAA TGGGCTACCT GCCAGTGCAG ACTGTCTTAG AGGGGGACAA CATGGAAACT 
CCTGTCACTC TGATCAACTT CTGGCCGGTA GATTCTGCGC CTGCCTCGTC CCCTCAGCTT 
TCACACGATG ATACTCATTC ACGCATTGAG CATTATGCTA GCAGGCTAGC AGAAATGGAA 
AACAGCAATG GATCTTATCT AAATGATAGC ATCTCTCCTA ATGAGAGCAT AGATGATGAA 
CATTTGTTAA TCCAGCATTA CTGCCAAAGT TTGAACCAGG ACTCCCCCCT GAGCCAGCCT 
CGTAGTCCTG CCCAGATCTT GATTTCCTTA GAGAGTGAGG AAAGAGGGGA GCTAGAGAGA 
ATCCTAGCAG ATCTTGAGGA AGAAAACAGA AATCTGCAAG CAGAGTATGA TCGTCTAAAG 
CAGCAGCATG AACACAAAGG CCTGTCCCCA CTGCCATCCC CTCCTGAAAT GATGCCTACT 
TCTCCCCAAA GTCCCCGGGA TGCTGAGCTC ATCGCTGAGG CCAAGCTGCT GCGTCAACAC 
AAAGGCCGCC TGGAAGCCAG GATGCAAATC CTTGAAGACC ATAACAAACA ACTGGAATCC 
CAGTTACACA GGCTCAGGCA GCTGCTGGAG CAACCCCAGG CAGAGGCCAA GGTGAATGGT 
ACAACGGTGT CTTCTCCTTC TACCTCTCTT CAGAGGTCAG ATAGCAGTCA GCCTATGCTG 
CTCCGGGTGG TCGGCAGTCA GACTTCAGAA TCCATGGGCG AGGAAGACCT GCTCAGCCCT 
CCCCAGGACA CAAGCACAGG GTTAGAGGAA GTGATGGAGC AGCTCAACCA CTCCTTCCCT 
AGTTCCAGAG GACACAATGT AGGAAGCCTT TTCCACTTGG CAGATGATTT GGGCAGAGCG 
ATGGAGTCCT TAGTTTCAGT CATGACAGAT GAAGAAGGGG CAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005641094.1
CDS	134..2041
Translation	MREQLKGHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQVINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPVTLINFWPVDSAPASSPQLSHDDTHSRIEHYASRLAEMENSNGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKQQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDTSTGLEEVMEQLNHSFPSSRGHNVGSLFHLADDLGRAMESLVSVMTDEEGAE

Target ORF information:

RefSeq Version	XM_005641037.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris dystrophin (DMD), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005641037.2

ORF Insert Sequence:

ATGAGGGAGC AGCTCAAAGG CCACGAGACC CAAACAACTT GCTGGGACCA TCCCAAAATG 
ACAGAGCTCT ACCAGTCTTT AGCTGACCTG AATAATGTCA GGTTCTCAGC TTACAGGACT 
GCCATGAAAC TCCGAAGACT GCAGAAGGCC CTTTGCTTGG ATCTCTTGAG CCTATCGGCT 
GCATGCGATG CCTTGGACCA GCACAACCTC AAGCAAAATG ACCAGCCCAT GGATATCCTG 
CAGGTCATTA ACTGTCTGAC CACTATTTAT GATCGCCTAG AGCAAGAGCA CAACAATCTG 
GTCAACGTCC CTCTCTGCGT GGATATGTGT CTCAATTGGC TGCTGAATGT TTATGACACG 
GGACGAACGG GGAGGATCCG GGTCCTGTCT TTTAAAACTG GCATCATTTC TCTGTGTAAA 
GCCCATTTGG AAGACAAGTA CAGATACCTC TTCAAGCAAG TGGCAAGTTC GACAGGATTT 
TGTGACCAGC GCAGGCTGGG CCTCCTCCTG CATGACTCTA TCCAGATCCC AAGACAGTTG 
GGTGAAGTCG CATCGTTCGG GGGCAGTAAC ATTGAGCCGA GTGTCAGGAG CTGCTTCCAG 
TTTGCTAATA ATAAGCCTGA GATCGAAGCG GCCCTCTTCC TAGACTGGAT GCGCCTGGAG 
CCCCAGTCCA TGGTGTGGCT GCCTGTCCTG CACCGAGTGG CTGCCGCGGA AACTGCCAAG 
CACCAGGCCA AGTGCAACAT CTGCAAGGAG TGTCCCATCA TCGGATTCAG GTACAGGAGT 
CTAAAGCACT TTAATTATGA CATCTGCCAA AGTTGCTTTT TTTCTGGTCG AGTTGCAAAA 
GGCCATAAAA TGCACTATCC CATGGTGGAA TACTGCACTC CGACTACATC GGGAGAAGAT 
GTCCGTGACT TTGCCAAGGT ACTAAAAAAC AAATTTCGAA CCAAAAGGTA TTTTGCGAAG 
CATCCCCGAA TGGGCTACCT GCCAGTGCAG ACTGTCTTAG AGGGGGACAA CATGGAAACT 
CCTGTCACTC TGATCAACTT CTGGCCGGTA GATTCTGCGC CTGCCTCGTC CCCTCAGCTT 
TCACACGATG ATACTCATTC ACGCATTGAG CATTATGCTA GCAGGCTAGC AGAAATGGAA 
AACAGCAATG GATCTTATCT AAATGATAGC ATCTCTCCTA ATGAGAGCAT AGATGATGAA 
CATTTGTTAA TCCAGCATTA CTGCCAAAGT TTGAACCAGG ACTCCCCCCT GAGCCAGCCT 
CGTAGTCCTG CCCAGATCTT GATTTCCTTA GAGAGTGAGG AAAGAGGGGA GCTAGAGAGA 
ATCCTAGCAG ATCTTGAGGA AGAAAACAGA AATCTGCAAG CAGAGTATGA TCGTCTAAAG 
CAGCAGCATG AACACAAAGG CCTGTCCCCA CTGCCATCCC CTCCTGAAAT GATGCCTACT 
TCTCCCCAAA GTCCCCGGGA TGCTGAGCTC ATCGCTGAGG CCAAGCTGCT GCGTCAACAC 
AAAGGCCGCC TGGAAGCCAG GATGCAAATC CTTGAAGACC ATAACAAACA ACTGGAATCC 
CAGTTACACA GGCTCAGGCA GCTGCTGGAG CAACCCCAGG CAGAGGCCAA GGTGAATGGT 
ACAACGGTGT CTTCTCCTTC TACCTCTCTT CAGAGGTCAG ATAGCAGTCA GCCTATGCTG 
CTCCGGGTGG TCGGCAGTCA GACTTCAGAA TCCATGGGCG AGGAAGACCT GCTCAGCCCT 
CCCCAGGACA CAAGCACAGG GTTAGAGGAA GTGATGGAGC AGCTCAACCA CTCCTTCCCT 
AGTTCCAGAG GACACAATGT AGGAAGCCTT TTCCACTTGG CAGATGATTT GGGCAGAGCG 
ATGGAGTCCT TAGTTTCAGT CATGACAGAT GAAGAAGGGG CAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMD ( XM_005641037.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005641094.1 Canis lupus familiaris dystrophin (DMD), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OCe31981
Clone ID Related Accession (Same CDS sequence)	XM_005641037.2 , XM_005641037.1
Accession Version	XM_005641037.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1908bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	dystrophin isoform X12
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGAGC AGCTCAAAGG CCACGAGACC CAAACAACTT GCTGGGACCA TCCCAAAATG 
ACAGAGCTCT ACCAGTCTTT AGCTGACCTG AATAATGTCA GGTTCTCAGC TTACAGGACT 
GCCATGAAAC TCCGAAGACT GCAGAAGGCC CTTTGCTTGG ATCTCTTGAG CCTATCGGCT 
GCATGCGATG CCTTGGACCA GCACAACCTC AAGCAAAATG ACCAGCCCAT GGATATCCTG 
CAGGTCATTA ACTGTCTGAC CACTATTTAT GATCGCCTAG AGCAAGAGCA CAACAATCTG 
GTCAACGTCC CTCTCTGCGT GGATATGTGT CTCAATTGGC TGCTGAATGT TTATGACACG 
GGACGAACGG GGAGGATCCG GGTCCTGTCT TTTAAAACTG GCATCATTTC TCTGTGTAAA 
GCCCATTTGG AAGACAAGTA CAGATACCTC TTCAAGCAAG TGGCAAGTTC GACAGGATTT 
TGTGACCAGC GCAGGCTGGG CCTCCTCCTG CATGACTCTA TCCAGATCCC AAGACAGTTG 
GGTGAAGTCG CATCGTTCGG GGGCAGTAAC ATTGAGCCGA GTGTCAGGAG CTGCTTCCAG 
TTTGCTAATA ATAAGCCTGA GATCGAAGCG GCCCTCTTCC TAGACTGGAT GCGCCTGGAG 
CCCCAGTCCA TGGTGTGGCT GCCTGTCCTG CACCGAGTGG CTGCCGCGGA AACTGCCAAG 
CACCAGGCCA AGTGCAACAT CTGCAAGGAG TGTCCCATCA TCGGATTCAG GTACAGGAGT 
CTAAAGCACT TTAATTATGA CATCTGCCAA AGTTGCTTTT TTTCTGGTCG AGTTGCAAAA 
GGCCATAAAA TGCACTATCC CATGGTGGAA TACTGCACTC CGACTACATC GGGAGAAGAT 
GTCCGTGACT TTGCCAAGGT ACTAAAAAAC AAATTTCGAA CCAAAAGGTA TTTTGCGAAG 
CATCCCCGAA TGGGCTACCT GCCAGTGCAG ACTGTCTTAG AGGGGGACAA CATGGAAACT 
CCTGTCACTC TGATCAACTT CTGGCCGGTA GATTCTGCGC CTGCCTCGTC CCCTCAGCTT 
TCACACGATG ATACTCATTC ACGCATTGAG CATTATGCTA GCAGGCTAGC AGAAATGGAA 
AACAGCAATG GATCTTATCT AAATGATAGC ATCTCTCCTA ATGAGAGCAT AGATGATGAA 
CATTTGTTAA TCCAGCATTA CTGCCAAAGT TTGAACCAGG ACTCCCCCCT GAGCCAGCCT 
CGTAGTCCTG CCCAGATCTT GATTTCCTTA GAGAGTGAGG AAAGAGGGGA GCTAGAGAGA 
ATCCTAGCAG ATCTTGAGGA AGAAAACAGA AATCTGCAAG CAGAGTATGA TCGTCTAAAG 
CAGCAGCATG AACACAAAGG CCTGTCCCCA CTGCCATCCC CTCCTGAAAT GATGCCTACT 
TCTCCCCAAA GTCCCCGGGA TGCTGAGCTC ATCGCTGAGG CCAAGCTGCT GCGTCAACAC 
AAAGGCCGCC TGGAAGCCAG GATGCAAATC CTTGAAGACC ATAACAAACA ACTGGAATCC 
CAGTTACACA GGCTCAGGCA GCTGCTGGAG CAACCCCAGG CAGAGGCCAA GGTGAATGGT 
ACAACGGTGT CTTCTCCTTC TACCTCTCTT CAGAGGTCAG ATAGCAGTCA GCCTATGCTG 
CTCCGGGTGG TCGGCAGTCA GACTTCAGAA TCCATGGGCG AGGAAGACCT GCTCAGCCCT 
CCCCAGGACA CAAGCACAGG GTTAGAGGAA GTGATGGAGC AGCTCAACCA CTCCTTCCCT 
AGTTCCAGAG GACACAATGT AGGAAGCCTT TTCCACTTGG CAGATGATTT GGGCAGAGCG 
ATGGAGTCCT TAGTTTCAGT CATGACAGAT GAAGAAGGGG CAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005641094.1
CDS	131..2038
Translation	MREQLKGHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQVINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPVTLINFWPVDSAPASSPQLSHDDTHSRIEHYASRLAEMENSNGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKQQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDTSTGLEEVMEQLNHSFPSSRGHNVGSLFHLADDLGRAMESLVSVMTDEEGAE

Target ORF information:

RefSeq Version	XM_005641037.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris dystrophin (DMD), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005641037.1

ORF Insert Sequence:

ATGAGGGAGC AGCTCAAAGG CCACGAGACC CAAACAACTT GCTGGGACCA TCCCAAAATG 
ACAGAGCTCT ACCAGTCTTT AGCTGACCTG AATAATGTCA GGTTCTCAGC TTACAGGACT 
GCCATGAAAC TCCGAAGACT GCAGAAGGCC CTTTGCTTGG ATCTCTTGAG CCTATCGGCT 
GCATGCGATG CCTTGGACCA GCACAACCTC AAGCAAAATG ACCAGCCCAT GGATATCCTG 
CAGGTCATTA ACTGTCTGAC CACTATTTAT GATCGCCTAG AGCAAGAGCA CAACAATCTG 
GTCAACGTCC CTCTCTGCGT GGATATGTGT CTCAATTGGC TGCTGAATGT TTATGACACG 
GGACGAACGG GGAGGATCCG GGTCCTGTCT TTTAAAACTG GCATCATTTC TCTGTGTAAA 
GCCCATTTGG AAGACAAGTA CAGATACCTC TTCAAGCAAG TGGCAAGTTC GACAGGATTT 
TGTGACCAGC GCAGGCTGGG CCTCCTCCTG CATGACTCTA TCCAGATCCC AAGACAGTTG 
GGTGAAGTCG CATCGTTCGG GGGCAGTAAC ATTGAGCCGA GTGTCAGGAG CTGCTTCCAG 
TTTGCTAATA ATAAGCCTGA GATCGAAGCG GCCCTCTTCC TAGACTGGAT GCGCCTGGAG 
CCCCAGTCCA TGGTGTGGCT GCCTGTCCTG CACCGAGTGG CTGCCGCGGA AACTGCCAAG 
CACCAGGCCA AGTGCAACAT CTGCAAGGAG TGTCCCATCA TCGGATTCAG GTACAGGAGT 
CTAAAGCACT TTAATTATGA CATCTGCCAA AGTTGCTTTT TTTCTGGTCG AGTTGCAAAA 
GGCCATAAAA TGCACTATCC CATGGTGGAA TACTGCACTC CGACTACATC GGGAGAAGAT 
GTCCGTGACT TTGCCAAGGT ACTAAAAAAC AAATTTCGAA CCAAAAGGTA TTTTGCGAAG 
CATCCCCGAA TGGGCTACCT GCCAGTGCAG ACTGTCTTAG AGGGGGACAA CATGGAAACT 
CCTGTCACTC TGATCAACTT CTGGCCGGTA GATTCTGCGC CTGCCTCGTC CCCTCAGCTT 
TCACACGATG ATACTCATTC ACGCATTGAG CATTATGCTA GCAGGCTAGC AGAAATGGAA 
AACAGCAATG GATCTTATCT AAATGATAGC ATCTCTCCTA ATGAGAGCAT AGATGATGAA 
CATTTGTTAA TCCAGCATTA CTGCCAAAGT TTGAACCAGG ACTCCCCCCT GAGCCAGCCT 
CGTAGTCCTG CCCAGATCTT GATTTCCTTA GAGAGTGAGG AAAGAGGGGA GCTAGAGAGA 
ATCCTAGCAG ATCTTGAGGA AGAAAACAGA AATCTGCAAG CAGAGTATGA TCGTCTAAAG 
CAGCAGCATG AACACAAAGG CCTGTCCCCA CTGCCATCCC CTCCTGAAAT GATGCCTACT 
TCTCCCCAAA GTCCCCGGGA TGCTGAGCTC ATCGCTGAGG CCAAGCTGCT GCGTCAACAC 
AAAGGCCGCC TGGAAGCCAG GATGCAAATC CTTGAAGACC ATAACAAACA ACTGGAATCC 
CAGTTACACA GGCTCAGGCA GCTGCTGGAG CAACCCCAGG CAGAGGCCAA GGTGAATGGT 
ACAACGGTGT CTTCTCCTTC TACCTCTCTT CAGAGGTCAG ATAGCAGTCA GCCTATGCTG 
CTCCGGGTGG TCGGCAGTCA GACTTCAGAA TCCATGGGCG AGGAAGACCT GCTCAGCCCT 
CCCCAGGACA CAAGCACAGG GTTAGAGGAA GTGATGGAGC AGCTCAACCA CTCCTTCCCT 
AGTTCCAGAG GACACAATGT AGGAAGCCTT TTCCACTTGG CAGATGATTT GGGCAGAGCG 
ATGGAGTCCT TAGTTTCAGT CATGACAGAT GAAGAAGGGG CAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMD ( XM_005641038.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005641095.1 Canis lupus familiaris dystrophin (DMD), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OCe31982
Clone ID Related Accession (Same CDS sequence)	XM_005641038.1 , XM_005641038.2
Accession Version	XM_005641038.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	dystrophin isoform X13
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGAGC AGCTCAAAGG CCACGAGACC CAAACAACTT GCTGGGACCA TCCCAAAATG 
ACAGAGCTCT ACCAGTCTTT AGCTGACCTG AATAATGTCA GGTTCTCAGC TTACAGGACT 
GCCATGAAAC TCCGAAGACT GCAGAAGGCC CTTTGCTTGG ATCTCTTGAG CCTATCGGCT 
GCATGCGATG CCTTGGACCA GCACAACCTC AAGCAAAATG ACCAGCCCAT GGATATCCTG 
CAGGTCATTA ACTGTCTGAC CACTATTTAT GATCGCCTAG AGCAAGAGCA CAACAATCTG 
GTCAACGTCC CTCTCTGCGT GGATATGTGT CTCAATTGGC TGCTGAATGT TTATGACACG 
GGACGAACGG GGAGGATCCG GGTCCTGTCT TTTAAAACTG GCATCATTTC TCTGTGTAAA 
GCCCATTTGG AAGACAAGTA CAGATACCTC TTCAAGCAAG TGGCAAGTTC GACAGGATTT 
TGTGACCAGC GCAGGCTGGG CCTCCTCCTG CATGACTCTA TCCAGATCCC AAGACAGTTG 
GGTGAAGTCG CATCGTTCGG GGGCAGTAAC ATTGAGCCGA GTGTCAGGAG CTGCTTCCAG 
TTTGCTAATA ATAAGCCTGA GATCGAAGCG GCCCTCTTCC TAGACTGGAT GCGCCTGGAG 
CCCCAGTCCA TGGTGTGGCT GCCTGTCCTG CACCGAGTGG CTGCCGCGGA AACTGCCAAG 
CACCAGGCCA AGTGCAACAT CTGCAAGGAG TGTCCCATCA TCGGATTCAG GTACAGGAGT 
CTAAAGCACT TTAATTATGA CATCTGCCAA AGTTGCTTTT TTTCTGGTCG AGTTGCAAAA 
GGCCATAAAA TGCACTATCC CATGGTGGAA TACTGCACTC CGACTACATC GGGAGAAGAT 
GTCCGTGACT TTGCCAAGGT ACTAAAAAAC AAATTTCGAA CCAAAAGGTA TTTTGCGAAG 
CATCCCCGAA TGGGCTACCT GCCAGTGCAG ACTGTCTTAG AGGGGGACAA CATGGAAACG 
CCTGCCTCGT CCCCTCAGCT TTCACACGAT GATACTCATT CACGCATTGA GCATTATGCT 
AGCAGGCTAG CAGAAATGGA AAACAGCAAT GGATCTTATC TAAATGATAG CATCTCTCCT 
AATGAGAGCA TAGATGATGA ACATTTGTTA ATCCAGCATT ACTGCCAAAG TTTGAACCAG 
GACTCCCCCC TGAGCCAGCC TCGTAGTCCT GCCCAGATCT TGATTTCCTT AGAGAGTGAG 
GAAAGAGGGG AGCTAGAGAG AATCCTAGCA GATCTTGAGG AAGAAAACAG AAATCTGCAA 
GCAGAGTATG ATCGTCTAAA GCAGCAGCAT GAACACAAAG GCCTGTCCCC ACTGCCATCC 
CCTCCTGAAA TGATGCCTAC TTCTCCCCAA AGTCCCCGGG ATGCTGAGCT CATCGCTGAG 
GCCAAGCTGC TGCGTCAACA CAAAGGCCGC CTGGAAGCCA GGATGCAAAT CCTTGAAGAC 
CATAACAAAC AACTGGAATC CCAGTTACAC AGGCTCAGGC AGCTGCTGGA GCAACCCCAG 
GCAGAGGCCA AGGTGAATGG TACAACGGTG TCTTCTCCTT CTACCTCTCT TCAGAGGTCA 
GATAGCAGTC AGCCTATGCT GCTCCGGGTG GTCGGCAGTC AGACTTCAGA ATCCATGGGC 
GAGGAAGACC TGCTCAGCCC TCCCCAGGAC ACAAGCACAG GGTTAGAGGA AGTGATGGAG 
CAGCTCAACC ACTCCTTCCC TAGTTCCAGA GGACACAATG TAGGAAGCCT TTTCCACTTG 
GCAGATGATT TGGGCAGAGC GATGGAGTCC TTAGTTTCAG TCATGACAGA TGAAGAAGGG 
GCAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005641095.1
CDS	131..1999
Translation	MREQLKGHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQVINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPASSPQLSHDDTHSRIEHYASRLAEMENSNGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKQQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDTSTGLEEVMEQLNHSFPSSRGHNVGSLFHLADDLGRAMESLVSVMTDEEGAE

Target ORF information:

RefSeq Version	XM_005641038.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris dystrophin (DMD), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005641038.1

ORF Insert Sequence:

ATGAGGGAGC AGCTCAAAGG CCACGAGACC CAAACAACTT GCTGGGACCA TCCCAAAATG 
ACAGAGCTCT ACCAGTCTTT AGCTGACCTG AATAATGTCA GGTTCTCAGC TTACAGGACT 
GCCATGAAAC TCCGAAGACT GCAGAAGGCC CTTTGCTTGG ATCTCTTGAG CCTATCGGCT 
GCATGCGATG CCTTGGACCA GCACAACCTC AAGCAAAATG ACCAGCCCAT GGATATCCTG 
CAGGTCATTA ACTGTCTGAC CACTATTTAT GATCGCCTAG AGCAAGAGCA CAACAATCTG 
GTCAACGTCC CTCTCTGCGT GGATATGTGT CTCAATTGGC TGCTGAATGT TTATGACACG 
GGACGAACGG GGAGGATCCG GGTCCTGTCT TTTAAAACTG GCATCATTTC TCTGTGTAAA 
GCCCATTTGG AAGACAAGTA CAGATACCTC TTCAAGCAAG TGGCAAGTTC GACAGGATTT 
TGTGACCAGC GCAGGCTGGG CCTCCTCCTG CATGACTCTA TCCAGATCCC AAGACAGTTG 
GGTGAAGTCG CATCGTTCGG GGGCAGTAAC ATTGAGCCGA GTGTCAGGAG CTGCTTCCAG 
TTTGCTAATA ATAAGCCTGA GATCGAAGCG GCCCTCTTCC TAGACTGGAT GCGCCTGGAG 
CCCCAGTCCA TGGTGTGGCT GCCTGTCCTG CACCGAGTGG CTGCCGCGGA AACTGCCAAG 
CACCAGGCCA AGTGCAACAT CTGCAAGGAG TGTCCCATCA TCGGATTCAG GTACAGGAGT 
CTAAAGCACT TTAATTATGA CATCTGCCAA AGTTGCTTTT TTTCTGGTCG AGTTGCAAAA 
GGCCATAAAA TGCACTATCC CATGGTGGAA TACTGCACTC CGACTACATC GGGAGAAGAT 
GTCCGTGACT TTGCCAAGGT ACTAAAAAAC AAATTTCGAA CCAAAAGGTA TTTTGCGAAG 
CATCCCCGAA TGGGCTACCT GCCAGTGCAG ACTGTCTTAG AGGGGGACAA CATGGAAACG 
CCTGCCTCGT CCCCTCAGCT TTCACACGAT GATACTCATT CACGCATTGA GCATTATGCT 
AGCAGGCTAG CAGAAATGGA AAACAGCAAT GGATCTTATC TAAATGATAG CATCTCTCCT 
AATGAGAGCA TAGATGATGA ACATTTGTTA ATCCAGCATT ACTGCCAAAG TTTGAACCAG 
GACTCCCCCC TGAGCCAGCC TCGTAGTCCT GCCCAGATCT TGATTTCCTT AGAGAGTGAG 
GAAAGAGGGG AGCTAGAGAG AATCCTAGCA GATCTTGAGG AAGAAAACAG AAATCTGCAA 
GCAGAGTATG ATCGTCTAAA GCAGCAGCAT GAACACAAAG GCCTGTCCCC ACTGCCATCC 
CCTCCTGAAA TGATGCCTAC TTCTCCCCAA AGTCCCCGGG ATGCTGAGCT CATCGCTGAG 
GCCAAGCTGC TGCGTCAACA CAAAGGCCGC CTGGAAGCCA GGATGCAAAT CCTTGAAGAC 
CATAACAAAC AACTGGAATC CCAGTTACAC AGGCTCAGGC AGCTGCTGGA GCAACCCCAG 
GCAGAGGCCA AGGTGAATGG TACAACGGTG TCTTCTCCTT CTACCTCTCT TCAGAGGTCA 
GATAGCAGTC AGCCTATGCT GCTCCGGGTG GTCGGCAGTC AGACTTCAGA ATCCATGGGC 
GAGGAAGACC TGCTCAGCCC TCCCCAGGAC ACAAGCACAG GGTTAGAGGA AGTGATGGAG 
CAGCTCAACC ACTCCTTCCC TAGTTCCAGA GGACACAATG TAGGAAGCCT TTTCCACTTG 
GCAGATGATT TGGGCAGAGC GATGGAGTCC TTAGTTTCAG TCATGACAGA TGAAGAAGGG 
GCAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMD ( XM_005641038.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005641095.1 Canis lupus familiaris dystrophin (DMD), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OCe31982
Clone ID Related Accession (Same CDS sequence)	XM_005641038.1 , XM_005641038.2
Accession Version	XM_005641038.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	dystrophin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005641038.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGAGC AGCTCAAAGG CCACGAGACC CAAACAACTT GCTGGGACCA TCCCAAAATG 
ACAGAGCTCT ACCAGTCTTT AGCTGACCTG AATAATGTCA GGTTCTCAGC TTACAGGACT 
GCCATGAAAC TCCGAAGACT GCAGAAGGCC CTTTGCTTGG ATCTCTTGAG CCTATCGGCT 
GCATGCGATG CCTTGGACCA GCACAACCTC AAGCAAAATG ACCAGCCCAT GGATATCCTG 
CAGGTCATTA ACTGTCTGAC CACTATTTAT GATCGCCTAG AGCAAGAGCA CAACAATCTG 
GTCAACGTCC CTCTCTGCGT GGATATGTGT CTCAATTGGC TGCTGAATGT TTATGACACG 
GGACGAACGG GGAGGATCCG GGTCCTGTCT TTTAAAACTG GCATCATTTC TCTGTGTAAA 
GCCCATTTGG AAGACAAGTA CAGATACCTC TTCAAGCAAG TGGCAAGTTC GACAGGATTT 
TGTGACCAGC GCAGGCTGGG CCTCCTCCTG CATGACTCTA TCCAGATCCC AAGACAGTTG 
GGTGAAGTCG CATCGTTCGG GGGCAGTAAC ATTGAGCCGA GTGTCAGGAG CTGCTTCCAG 
TTTGCTAATA ATAAGCCTGA GATCGAAGCG GCCCTCTTCC TAGACTGGAT GCGCCTGGAG 
CCCCAGTCCA TGGTGTGGCT GCCTGTCCTG CACCGAGTGG CTGCCGCGGA AACTGCCAAG 
CACCAGGCCA AGTGCAACAT CTGCAAGGAG TGTCCCATCA TCGGATTCAG GTACAGGAGT 
CTAAAGCACT TTAATTATGA CATCTGCCAA AGTTGCTTTT TTTCTGGTCG AGTTGCAAAA 
GGCCATAAAA TGCACTATCC CATGGTGGAA TACTGCACTC CGACTACATC GGGAGAAGAT 
GTCCGTGACT TTGCCAAGGT ACTAAAAAAC AAATTTCGAA CCAAAAGGTA TTTTGCGAAG 
CATCCCCGAA TGGGCTACCT GCCAGTGCAG ACTGTCTTAG AGGGGGACAA CATGGAAACG 
CCTGCCTCGT CCCCTCAGCT TTCACACGAT GATACTCATT CACGCATTGA GCATTATGCT 
AGCAGGCTAG CAGAAATGGA AAACAGCAAT GGATCTTATC TAAATGATAG CATCTCTCCT 
AATGAGAGCA TAGATGATGA ACATTTGTTA ATCCAGCATT ACTGCCAAAG TTTGAACCAG 
GACTCCCCCC TGAGCCAGCC TCGTAGTCCT GCCCAGATCT TGATTTCCTT AGAGAGTGAG 
GAAAGAGGGG AGCTAGAGAG AATCCTAGCA GATCTTGAGG AAGAAAACAG AAATCTGCAA 
GCAGAGTATG ATCGTCTAAA GCAGCAGCAT GAACACAAAG GCCTGTCCCC ACTGCCATCC 
CCTCCTGAAA TGATGCCTAC TTCTCCCCAA AGTCCCCGGG ATGCTGAGCT CATCGCTGAG 
GCCAAGCTGC TGCGTCAACA CAAAGGCCGC CTGGAAGCCA GGATGCAAAT CCTTGAAGAC 
CATAACAAAC AACTGGAATC CCAGTTACAC AGGCTCAGGC AGCTGCTGGA GCAACCCCAG 
GCAGAGGCCA AGGTGAATGG TACAACGGTG TCTTCTCCTT CTACCTCTCT TCAGAGGTCA 
GATAGCAGTC AGCCTATGCT GCTCCGGGTG GTCGGCAGTC AGACTTCAGA ATCCATGGGC 
GAGGAAGACC TGCTCAGCCC TCCCCAGGAC ACAAGCACAG GGTTAGAGGA AGTGATGGAG 
CAGCTCAACC ACTCCTTCCC TAGTTCCAGA GGACACAATG TAGGAAGCCT TTTCCACTTG 
GCAGATGATT TGGGCAGAGC GATGGAGTCC TTAGTTTCAG TCATGACAGA TGAAGAAGGG 
GCAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005641095.1
CDS	135..2003
Translation	MREQLKGHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQVINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPASSPQLSHDDTHSRIEHYASRLAEMENSNGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKQQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDTSTGLEEVMEQLNHSFPSSRGHNVGSLFHLADDLGRAMESLVSVMTDEEGAE

Target ORF information:

RefSeq Version	XM_005641038.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris dystrophin (DMD), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005641038.2

ORF Insert Sequence:

ATGAGGGAGC AGCTCAAAGG CCACGAGACC CAAACAACTT GCTGGGACCA TCCCAAAATG 
ACAGAGCTCT ACCAGTCTTT AGCTGACCTG AATAATGTCA GGTTCTCAGC TTACAGGACT 
GCCATGAAAC TCCGAAGACT GCAGAAGGCC CTTTGCTTGG ATCTCTTGAG CCTATCGGCT 
GCATGCGATG CCTTGGACCA GCACAACCTC AAGCAAAATG ACCAGCCCAT GGATATCCTG 
CAGGTCATTA ACTGTCTGAC CACTATTTAT GATCGCCTAG AGCAAGAGCA CAACAATCTG 
GTCAACGTCC CTCTCTGCGT GGATATGTGT CTCAATTGGC TGCTGAATGT TTATGACACG 
GGACGAACGG GGAGGATCCG GGTCCTGTCT TTTAAAACTG GCATCATTTC TCTGTGTAAA 
GCCCATTTGG AAGACAAGTA CAGATACCTC TTCAAGCAAG TGGCAAGTTC GACAGGATTT 
TGTGACCAGC GCAGGCTGGG CCTCCTCCTG CATGACTCTA TCCAGATCCC AAGACAGTTG 
GGTGAAGTCG CATCGTTCGG GGGCAGTAAC ATTGAGCCGA GTGTCAGGAG CTGCTTCCAG 
TTTGCTAATA ATAAGCCTGA GATCGAAGCG GCCCTCTTCC TAGACTGGAT GCGCCTGGAG 
CCCCAGTCCA TGGTGTGGCT GCCTGTCCTG CACCGAGTGG CTGCCGCGGA AACTGCCAAG 
CACCAGGCCA AGTGCAACAT CTGCAAGGAG TGTCCCATCA TCGGATTCAG GTACAGGAGT 
CTAAAGCACT TTAATTATGA CATCTGCCAA AGTTGCTTTT TTTCTGGTCG AGTTGCAAAA 
GGCCATAAAA TGCACTATCC CATGGTGGAA TACTGCACTC CGACTACATC GGGAGAAGAT 
GTCCGTGACT TTGCCAAGGT ACTAAAAAAC AAATTTCGAA CCAAAAGGTA TTTTGCGAAG 
CATCCCCGAA TGGGCTACCT GCCAGTGCAG ACTGTCTTAG AGGGGGACAA CATGGAAACG 
CCTGCCTCGT CCCCTCAGCT TTCACACGAT GATACTCATT CACGCATTGA GCATTATGCT 
AGCAGGCTAG CAGAAATGGA AAACAGCAAT GGATCTTATC TAAATGATAG CATCTCTCCT 
AATGAGAGCA TAGATGATGA ACATTTGTTA ATCCAGCATT ACTGCCAAAG TTTGAACCAG 
GACTCCCCCC TGAGCCAGCC TCGTAGTCCT GCCCAGATCT TGATTTCCTT AGAGAGTGAG 
GAAAGAGGGG AGCTAGAGAG AATCCTAGCA GATCTTGAGG AAGAAAACAG AAATCTGCAA 
GCAGAGTATG ATCGTCTAAA GCAGCAGCAT GAACACAAAG GCCTGTCCCC ACTGCCATCC 
CCTCCTGAAA TGATGCCTAC TTCTCCCCAA AGTCCCCGGG ATGCTGAGCT CATCGCTGAG 
GCCAAGCTGC TGCGTCAACA CAAAGGCCGC CTGGAAGCCA GGATGCAAAT CCTTGAAGAC 
CATAACAAAC AACTGGAATC CCAGTTACAC AGGCTCAGGC AGCTGCTGGA GCAACCCCAG 
GCAGAGGCCA AGGTGAATGG TACAACGGTG TCTTCTCCTT CTACCTCTCT TCAGAGGTCA 
GATAGCAGTC AGCCTATGCT GCTCCGGGTG GTCGGCAGTC AGACTTCAGA ATCCATGGGC 
GAGGAAGACC TGCTCAGCCC TCCCCAGGAC ACAAGCACAG GGTTAGAGGA AGTGATGGAG 
CAGCTCAACC ACTCCTTCCC TAGTTCCAGA GGACACAATG TAGGAAGCCT TTTCCACTTG 
GCAGATGATT TGGGCAGAGC GATGGAGTCC TTAGTTTCAG TCATGACAGA TGAAGAAGGG 
GCAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMD ( XM_022415521.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022271229.1 Canis lupus familiaris dystrophin (DMD), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OCe285080
Clone ID Related Accession (Same CDS sequence)	XM_022415521.1
Accession Version	XM_022415521.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2913bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	dystrophin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACAGG ATCAGTGCTG TAGTCCCCGG TTCAAGCTGA AAATGTTACA CAGGAAGACA 
TACCATGTAA AGGATCTCCA AGGAGAAATC GAAGCTCACA CAGATATCTA TCACAACCTG 
GACGAAAATG GCCAAAAAGT CCTGAGATCC CTGGAAGGTT CTGACGATGC AGCCTTGTTG 
CAAAGACGTT TGGATAACAT GAACTTCAAG TGGAGCGAAC TTCGGAAAAA GTCTCTCAAC 
ATTAGGTCTC ACTTGGAAGC CAGTTCTGAC CAGTGGAAGC GTCTGCACCT TTCTCTTCAG 
GAACTTCTGG TATGGCTCCA GCTGAAAGAT GATGAGTTAA GCCGGCAGGC ACCCATTGGA 
GGAGACTTTC CAGCGGTGCA GAAGCAGAAT GATGTACACA GGGCCTTCAA GAGGGAATTG 
AAAACGAAAG AACCTGTAAT CATGAGTACT CTTGAGACTG TACGAATATT TCTGACAGAG 
CAGCCTTTAG AAGGACTAGA GAAACTCTAC CAGGAGCCCA GAGAGCTGCC TCCTGAAGAG 
AGAGCCCAGA ATGTCACACG GCTCCTACGA AAGCAAGCTG AGGAGGTCAA CACTCAGTGG 
GAAAAACTGA ACGTGCACTC TGCAGACTGG CAGAGAAAAA TAGACGAGGC CCTCGAAAGA 
CTCCAGGAGC TTCAGGAAGC AACAGATGAG CTGGATCTCA AACTACGTCA GGCAGAGGTG 
ATCAAAGGAT CCTGGCAGCC TGTGGGTGAT CTCCTCATTG ACTCTCTCCA AGATCACCTC 
GAAAAAGTCA AGGCGCTTCG AGGAGAAATT ACACCTCTGA AAGAGAATGT CAGCTACGTC 
AATGACCTTG CTCGCCAACT CACTACGTTG GGCATTCAGC TGTCACCATA TAACCTCAAC 
ACTCTGGAAG ACCTGAACAC CAGATGGAAG CTTCTGCAGG TGGCCATTGA GGACCGCATC 
AGGCAGCTGC ATGAAGCGCA CAGGGACTTT GGACCAGCCT CCCAGCACTT CCTTTCCACT 
TCTGTCCAGG GTCCCTGGGA GAGAGCCATC TCACCAAACA AAGTGCCCTA CTATATCAAC 
CACGAGACCC AAACAACTTG CTGGGACCAT CCCAAAATGA CAGAGCTCTA CCAGTCTTTA 
GCTGACCTGA ATAATGTCAG GTTCTCAGCT TACAGGACTG CCATGAAACT CCGAAGACTG 
CAGAAGGCCC TTTGCTTGGA TCTCTTGAGC CTATCGGCTG CATGCGATGC CTTGGACCAG 
CACAACCTCA AGCAAAATGA CCAGCCCATG GATATCCTGC AGGTCATTAA CTGTCTGACC 
ACTATTTATG ATCGCCTAGA GCAAGAGCAC AACAATCTGG TCAACGTCCC TCTCTGCGTG 
GATATGTGTC TCAATTGGCT GCTGAATGTT TATGACACGG GACGAACGGG GAGGATCCGG 
GTCCTGTCTT TTAAAACTGG CATCATTTCT CTGTGTAAAG CCCATTTGGA AGACAAGTAC 
AGATACCTCT TCAAGCAAGT GGCAAGTTCG ACAGGATTTT GTGACCAGCG CAGGCTGGGC 
CTCCTCCTGC ATGACTCTAT CCAGATCCCA AGACAGTTGG GTGAAGTCGC ATCGTTCGGG 
GGCAGTAACA TTGAGCCGAG TGTCAGGAGC TGCTTCCAGT TTGCTAATAA TAAGCCTGAG 
ATCGAAGCGG CCCTCTTCCT AGACTGGATG CGCCTGGAGC CCCAGTCCAT GGTGTGGCTG 
CCTGTCCTGC ACCGAGTGGC TGCCGCGGAA ACTGCCAAGC ACCAGGCCAA GTGCAACATC 
TGCAAGGAGT GTCCCATCAT CGGATTCAGG TACAGGAGTC TAAAGCACTT TAATTATGAC 
ATCTGCCAAA GTTGCTTTTT TTCTGGTCGA GTTGCAAAAG GCCATAAAAT GCACTATCCC 
ATGGTGGAAT ACTGCACTCC GACTACATCG GGAGAAGATG TCCGTGACTT TGCCAAGGTA 
CTAAAAAACA AATTTCGAAC CAAAAGGTAT TTTGCGAAGC ATCCCCGAAT GGGCTACCTG 
CCAGTGCAGA CTGTCTTAGA GGGGGACAAC ATGGAAACTC CTGTCACTCT GATCAACTTC 
TGGCCGGTAG ATTCTGCGCC TGCCTCGTCC CCTCAGCTTT CACACGATGA TACTCATTCA 
CGCATTGAGC ATTATGCTAG CAGGCTAGCA GAAATGGAAA ACAGCAATGG ATCTTATCTA 
AATGATAGCA TCTCTCCTAA TGAGAGCATA GATGATGAAC ATTTGTTAAT CCAGCATTAC 
TGCCAAAGTT TGAACCAGGA CTCCCCCCTG AGCCAGCCTC GTAGTCCTGC CCAGATCTTG 
ATTTCCTTAG AGAGTGAGGA AAGAGGGGAG CTAGAGAGAA TCCTAGCAGA TCTTGAGGAA 
GAAAACAGAA ATCTGCAAGC AGAGTATGAT CGTCTAAAGC AGCAGCATGA ACACAAAGGC 
CTGTCCCCAC TGCCATCCCC TCCTGAAATG ATGCCTACTT CTCCCCAAAG TCCCCGGGAT 
GCTGAGCTCA TCGCTGAGGC CAAGCTGCTG CGTCAACACA AAGGCCGCCT GGAAGCCAGG 
ATGCAAATCC TTGAAGACCA TAACAAACAA CTGGAATCCC AGTTACACAG GCTCAGGCAG 
CTGCTGGAGC AACCCCAGGC AGAGGCCAAG GTGAATGGTA CAACGGTGTC TTCTCCTTCT 
ACCTCTCTTC AGAGGTCAGA TAGCAGTCAG CCTATGCTGC TCCGGGTGGT CGGCAGTCAG 
ACTTCAGAAT CCATGGGCGA GGAAGACCTG CTCAGCCCTC CCCAGGACAC AAGCACAGGG 
TTAGAGGAAG TGATGGAGCA GCTCAACCAC TCCTTCCCTA GTTCCAGAGG AAGAAATACC 
CCTGGGAAGC CAATGAGAGA GGACACAATG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022271229.1
CDS	20..2932
Translation	MQQDQCCSPRFKLKMLHRKTYHVKDLQGEIEAHTDIYHNLDENGQKVLRSLEGSDDAALLQRRLDNMNFKWSELRKKSLNIRSHLEASSDQWKRLHLSLQELLVWLQLKDDELSRQAPIGGDFPAVQKQNDVHRAFKRELKTKEPVIMSTLETVRIFLTEQPLEGLEKLYQEPRELPPEERAQNVTRLLRKQAEEVNTQWEKLNVHSADWQRKIDEALERLQELQEATDELDLKLRQAEVIKGSWQPVGDLLIDSLQDHLEKVKALRGEITPLKENVSYVNDLARQLTTLGIQLSPYNLNTLEDLNTRWKLLQVAIEDRIRQLHEAHRDFGPASQHFLSTSVQGPWERAISPNKVPYYINHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQVINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPVTLINFWPVDSAPASSPQLSHDDTHSRIEHYASRLAEMENSNGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKQQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDTSTGLEEVMEQLNHSFPSSRGRNTPGKPMREDTM

Target ORF information:

RefSeq Version	XM_022415521.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris dystrophin (DMD), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022415521.1

ORF Insert Sequence:

ATGCAACAGG ATCAGTGCTG TAGTCCCCGG TTCAAGCTGA AAATGTTACA CAGGAAGACA 
TACCATGTAA AGGATCTCCA AGGAGAAATC GAAGCTCACA CAGATATCTA TCACAACCTG 
GACGAAAATG GCCAAAAAGT CCTGAGATCC CTGGAAGGTT CTGACGATGC AGCCTTGTTG 
CAAAGACGTT TGGATAACAT GAACTTCAAG TGGAGCGAAC TTCGGAAAAA GTCTCTCAAC 
ATTAGGTCTC ACTTGGAAGC CAGTTCTGAC CAGTGGAAGC GTCTGCACCT TTCTCTTCAG 
GAACTTCTGG TATGGCTCCA GCTGAAAGAT GATGAGTTAA GCCGGCAGGC ACCCATTGGA 
GGAGACTTTC CAGCGGTGCA GAAGCAGAAT GATGTACACA GGGCCTTCAA GAGGGAATTG 
AAAACGAAAG AACCTGTAAT CATGAGTACT CTTGAGACTG TACGAATATT TCTGACAGAG 
CAGCCTTTAG AAGGACTAGA GAAACTCTAC CAGGAGCCCA GAGAGCTGCC TCCTGAAGAG 
AGAGCCCAGA ATGTCACACG GCTCCTACGA AAGCAAGCTG AGGAGGTCAA CACTCAGTGG 
GAAAAACTGA ACGTGCACTC TGCAGACTGG CAGAGAAAAA TAGACGAGGC CCTCGAAAGA 
CTCCAGGAGC TTCAGGAAGC AACAGATGAG CTGGATCTCA AACTACGTCA GGCAGAGGTG 
ATCAAAGGAT CCTGGCAGCC TGTGGGTGAT CTCCTCATTG ACTCTCTCCA AGATCACCTC 
GAAAAAGTCA AGGCGCTTCG AGGAGAAATT ACACCTCTGA AAGAGAATGT CAGCTACGTC 
AATGACCTTG CTCGCCAACT CACTACGTTG GGCATTCAGC TGTCACCATA TAACCTCAAC 
ACTCTGGAAG ACCTGAACAC CAGATGGAAG CTTCTGCAGG TGGCCATTGA GGACCGCATC 
AGGCAGCTGC ATGAAGCGCA CAGGGACTTT GGACCAGCCT CCCAGCACTT CCTTTCCACT 
TCTGTCCAGG GTCCCTGGGA GAGAGCCATC TCACCAAACA AAGTGCCCTA CTATATCAAC 
CACGAGACCC AAACAACTTG CTGGGACCAT CCCAAAATGA CAGAGCTCTA CCAGTCTTTA 
GCTGACCTGA ATAATGTCAG GTTCTCAGCT TACAGGACTG CCATGAAACT CCGAAGACTG 
CAGAAGGCCC TTTGCTTGGA TCTCTTGAGC CTATCGGCTG CATGCGATGC CTTGGACCAG 
CACAACCTCA AGCAAAATGA CCAGCCCATG GATATCCTGC AGGTCATTAA CTGTCTGACC 
ACTATTTATG ATCGCCTAGA GCAAGAGCAC AACAATCTGG TCAACGTCCC TCTCTGCGTG 
GATATGTGTC TCAATTGGCT GCTGAATGTT TATGACACGG GACGAACGGG GAGGATCCGG 
GTCCTGTCTT TTAAAACTGG CATCATTTCT CTGTGTAAAG CCCATTTGGA AGACAAGTAC 
AGATACCTCT TCAAGCAAGT GGCAAGTTCG ACAGGATTTT GTGACCAGCG CAGGCTGGGC 
CTCCTCCTGC ATGACTCTAT CCAGATCCCA AGACAGTTGG GTGAAGTCGC ATCGTTCGGG 
GGCAGTAACA TTGAGCCGAG TGTCAGGAGC TGCTTCCAGT TTGCTAATAA TAAGCCTGAG 
ATCGAAGCGG CCCTCTTCCT AGACTGGATG CGCCTGGAGC CCCAGTCCAT GGTGTGGCTG 
CCTGTCCTGC ACCGAGTGGC TGCCGCGGAA ACTGCCAAGC ACCAGGCCAA GTGCAACATC 
TGCAAGGAGT GTCCCATCAT CGGATTCAGG TACAGGAGTC TAAAGCACTT TAATTATGAC 
ATCTGCCAAA GTTGCTTTTT TTCTGGTCGA GTTGCAAAAG GCCATAAAAT GCACTATCCC 
ATGGTGGAAT ACTGCACTCC GACTACATCG GGAGAAGATG TCCGTGACTT TGCCAAGGTA 
CTAAAAAACA AATTTCGAAC CAAAAGGTAT TTTGCGAAGC ATCCCCGAAT GGGCTACCTG 
CCAGTGCAGA CTGTCTTAGA GGGGGACAAC ATGGAAACTC CTGTCACTCT GATCAACTTC 
TGGCCGGTAG ATTCTGCGCC TGCCTCGTCC CCTCAGCTTT CACACGATGA TACTCATTCA 
CGCATTGAGC ATTATGCTAG CAGGCTAGCA GAAATGGAAA ACAGCAATGG ATCTTATCTA 
AATGATAGCA TCTCTCCTAA TGAGAGCATA GATGATGAAC ATTTGTTAAT CCAGCATTAC 
TGCCAAAGTT TGAACCAGGA CTCCCCCCTG AGCCAGCCTC GTAGTCCTGC CCAGATCTTG 
ATTTCCTTAG AGAGTGAGGA AAGAGGGGAG CTAGAGAGAA TCCTAGCAGA TCTTGAGGAA 
GAAAACAGAA ATCTGCAAGC AGAGTATGAT CGTCTAAAGC AGCAGCATGA ACACAAAGGC 
CTGTCCCCAC TGCCATCCCC TCCTGAAATG ATGCCTACTT CTCCCCAAAG TCCCCGGGAT 
GCTGAGCTCA TCGCTGAGGC CAAGCTGCTG CGTCAACACA AAGGCCGCCT GGAAGCCAGG 
ATGCAAATCC TTGAAGACCA TAACAAACAA CTGGAATCCC AGTTACACAG GCTCAGGCAG 
CTGCTGGAGC AACCCCAGGC AGAGGCCAAG GTGAATGGTA CAACGGTGTC TTCTCCTTCT 
ACCTCTCTTC AGAGGTCAGA TAGCAGTCAG CCTATGCTGC TCCGGGTGGT CGGCAGTCAG 
ACTTCAGAAT CCATGGGCGA GGAAGACCTG CTCAGCCCTC CCCAGGACAC AAGCACAGGG 
TTAGAGGAAG TGATGGAGCA GCTCAACCAC TCCTTCCCTA GTTCCAGAGG AAGAAATACC 
CCTGGGAAGC CAATGAGAGA GGACACAATG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMD ( XM_014111350.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013966825.1 Canis lupus familiaris dystrophin (DMD), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OCe45421
Clone ID Related Accession (Same CDS sequence)	XM_014111350.2 , XM_014111350.1
Accession Version	XM_014111350.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	dystrophin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014111350.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGAGC AGCTCAAAGG CCACGAGACC CAAACAACTT GCTGGGACCA TCCCAAAATG 
ACAGAGCTCT ACCAGTCTTT AGCTGACCTG AATAATGTCA GGTTCTCAGC TTACAGGACT 
GCCATGAAAC TCCGAAGACT GCAGAAGGCC CTTTGCTTGG ATCTCTTGAG CCTATCGGCT 
GCATGCGATG CCTTGGACCA GCACAACCTC AAGCAAAATG ACCAGCCCAT GGATATCCTG 
CAGGTCATTA ACTGTCTGAC CACTATTTAT GATCGCCTAG AGCAAGAGCA CAACAATCTG 
GTCAACGTCC CTCTCTGCGT GGATATGTGT CTCAATTGGC TGCTGAATGT TTATGACACG 
GGACGAACGG GGAGGATCCG GGTCCTGTCT TTTAAAACTG GCATCATTTC TCTGTGTAAA 
GCCCATTTGG AAGACAAGTA CAGATACCTC TTCAAGCAAG TGGCAAGTTC GACAGGATTT 
TGTGACCAGC GCAGGCTGGG CCTCCTCCTG CATGACTCTA TCCAGATCCC AAGACAGTTG 
GGTGAAGTCG CATCGTTCGG GGGCAGTAAC ATTGAGCCGA GTGTCAGGAG CTGCTTCCAG 
TTTGCTAATA ATAAGCCTGA GATCGAAGCG GCCCTCTTCC TAGACTGGAT GCGCCTGGAG 
CCCCAGTCCA TGGTGTGGCT GCCTGTCCTG CACCGAGTGG CTGCCGCGGA AACTGCCAAG 
CACCAGGCCA AGTGCAACAT CTGCAAGGAG TGTCCCATCA TCGGATTCAG GTACAGGAGT 
CTAAAGCACT TTAATTATGA CATCTGCCAA AGTTGCTTTT TTTCTGGTCG AGTTGCAAAA 
GGCCATAAAA TGCACTATCC CATGGTGGAA TACTGCACTC CGACTACATC GGGAGAAGAT 
GTCCGTGACT TTGCCAAGGT ACTAAAAAAC AAATTTCGAA CCAAAAGGTA TTTTGCGAAG 
CATCCCCGAA TGGGCTACCT GCCAGTGCAG ACTGTCTTAG AGGGGGACAA CATGGAAACT 
CCTGTCACTC TGATCAACTT CTGGCCGGTA GATTCTGCGC CTGCCTCGTC CCCTCAGCTT 
TCACACGATG ATACTCATTC ACGCATTGAG CATTATGCTA GCAGGCTAGC AGAAATGGAA 
AACAGCAATG GATCTTATCT AAATGATAGC ATCTCTCCTA ATGAGAGCAT AGATGATGAA 
CATTTGTTAA TCCAGCATTA CTGCCAAAGT TTGAACCAGG ACTCCCCCCT GAGCCAGCCT 
CGTAGTCCTG CCCAGATCTT GATTTCCTTA GAGAGTGAGG AAAGAGGGGA GCTAGAGAGA 
ATCCTAGCAG ATCTTGAGGA AGAAAACAGA AATCTGCAAG CAGAGTATGA TCGTCTAAAG 
CAGCAGCATG AACACAAAGG CCTGTCCCCA CTGCCATCCC CTCCTGAAAT GATGCCTACT 
TCTCCCCAAA GTCCCCGGGA TGCTGAGCTC ATCGCTGAGG CCAAGCTGCT GCGTCAACAC 
AAAGGCCGCC TGGAAGCCAG GATGCAAATC CTTGAAGACC ATAACAAACA ACTGGAATCC 
CAGTTACACA GGCTCAGGCA GCTGCTGGAG CAACCCCAGG CAGAGGCCAA GGTGAATGGT 
ACAACGGTGT CTTCTCCTTC TACCTCTCTT CAGAGGTCAG ATAGCAGTCA GCCTATGCTG 
CTCCGGGTGG TCGGCAGTCA GACTTCAGAA TCCATGGGCG AGGAAGACCT GCTCAGCCCT 
CCCCAGGACA CAAGCACAGG GTTAGAGGAA GTGATGGAGC AGCTCAACCA CTCCTTCCCT 
AGTTCCAGAG GAAGAAATAC CCCTGGGAAG CCAATGAGAG AGGACACAAT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013966825.1
CDS	134..1987
Translation	MREQLKGHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQVINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPVTLINFWPVDSAPASSPQLSHDDTHSRIEHYASRLAEMENSNGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKQQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDTSTGLEEVMEQLNHSFPSSRGRNTPGKPMREDTM

Target ORF information:

RefSeq Version	XM_014111350.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris dystrophin (DMD), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111350.2

ORF Insert Sequence:

ATGAGGGAGC AGCTCAAAGG CCACGAGACC CAAACAACTT GCTGGGACCA TCCCAAAATG 
ACAGAGCTCT ACCAGTCTTT AGCTGACCTG AATAATGTCA GGTTCTCAGC TTACAGGACT 
GCCATGAAAC TCCGAAGACT GCAGAAGGCC CTTTGCTTGG ATCTCTTGAG CCTATCGGCT 
GCATGCGATG CCTTGGACCA GCACAACCTC AAGCAAAATG ACCAGCCCAT GGATATCCTG 
CAGGTCATTA ACTGTCTGAC CACTATTTAT GATCGCCTAG AGCAAGAGCA CAACAATCTG 
GTCAACGTCC CTCTCTGCGT GGATATGTGT CTCAATTGGC TGCTGAATGT TTATGACACG 
GGACGAACGG GGAGGATCCG GGTCCTGTCT TTTAAAACTG GCATCATTTC TCTGTGTAAA 
GCCCATTTGG AAGACAAGTA CAGATACCTC TTCAAGCAAG TGGCAAGTTC GACAGGATTT 
TGTGACCAGC GCAGGCTGGG CCTCCTCCTG CATGACTCTA TCCAGATCCC AAGACAGTTG 
GGTGAAGTCG CATCGTTCGG GGGCAGTAAC ATTGAGCCGA GTGTCAGGAG CTGCTTCCAG 
TTTGCTAATA ATAAGCCTGA GATCGAAGCG GCCCTCTTCC TAGACTGGAT GCGCCTGGAG 
CCCCAGTCCA TGGTGTGGCT GCCTGTCCTG CACCGAGTGG CTGCCGCGGA AACTGCCAAG 
CACCAGGCCA AGTGCAACAT CTGCAAGGAG TGTCCCATCA TCGGATTCAG GTACAGGAGT 
CTAAAGCACT TTAATTATGA CATCTGCCAA AGTTGCTTTT TTTCTGGTCG AGTTGCAAAA 
GGCCATAAAA TGCACTATCC CATGGTGGAA TACTGCACTC CGACTACATC GGGAGAAGAT 
GTCCGTGACT TTGCCAAGGT ACTAAAAAAC AAATTTCGAA CCAAAAGGTA TTTTGCGAAG 
CATCCCCGAA TGGGCTACCT GCCAGTGCAG ACTGTCTTAG AGGGGGACAA CATGGAAACT 
CCTGTCACTC TGATCAACTT CTGGCCGGTA GATTCTGCGC CTGCCTCGTC CCCTCAGCTT 
TCACACGATG ATACTCATTC ACGCATTGAG CATTATGCTA GCAGGCTAGC AGAAATGGAA 
AACAGCAATG GATCTTATCT AAATGATAGC ATCTCTCCTA ATGAGAGCAT AGATGATGAA 
CATTTGTTAA TCCAGCATTA CTGCCAAAGT TTGAACCAGG ACTCCCCCCT GAGCCAGCCT 
CGTAGTCCTG CCCAGATCTT GATTTCCTTA GAGAGTGAGG AAAGAGGGGA GCTAGAGAGA 
ATCCTAGCAG ATCTTGAGGA AGAAAACAGA AATCTGCAAG CAGAGTATGA TCGTCTAAAG 
CAGCAGCATG AACACAAAGG CCTGTCCCCA CTGCCATCCC CTCCTGAAAT GATGCCTACT 
TCTCCCCAAA GTCCCCGGGA TGCTGAGCTC ATCGCTGAGG CCAAGCTGCT GCGTCAACAC 
AAAGGCCGCC TGGAAGCCAG GATGCAAATC CTTGAAGACC ATAACAAACA ACTGGAATCC 
CAGTTACACA GGCTCAGGCA GCTGCTGGAG CAACCCCAGG CAGAGGCCAA GGTGAATGGT 
ACAACGGTGT CTTCTCCTTC TACCTCTCTT CAGAGGTCAG ATAGCAGTCA GCCTATGCTG 
CTCCGGGTGG TCGGCAGTCA GACTTCAGAA TCCATGGGCG AGGAAGACCT GCTCAGCCCT 
CCCCAGGACA CAAGCACAGG GTTAGAGGAA GTGATGGAGC AGCTCAACCA CTCCTTCCCT 
AGTTCCAGAG GAAGAAATAC CCCTGGGAAG CCAATGAGAG AGGACACAAT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMD ( XM_014111350.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013966825.1 Canis lupus familiaris dystrophin (DMD), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OCe45421
Clone ID Related Accession (Same CDS sequence)	XM_014111350.2 , XM_014111350.1
Accession Version	XM_014111350.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	dystrophin isoform X14
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGAGC AGCTCAAAGG CCACGAGACC CAAACAACTT GCTGGGACCA TCCCAAAATG 
ACAGAGCTCT ACCAGTCTTT AGCTGACCTG AATAATGTCA GGTTCTCAGC TTACAGGACT 
GCCATGAAAC TCCGAAGACT GCAGAAGGCC CTTTGCTTGG ATCTCTTGAG CCTATCGGCT 
GCATGCGATG CCTTGGACCA GCACAACCTC AAGCAAAATG ACCAGCCCAT GGATATCCTG 
CAGGTCATTA ACTGTCTGAC CACTATTTAT GATCGCCTAG AGCAAGAGCA CAACAATCTG 
GTCAACGTCC CTCTCTGCGT GGATATGTGT CTCAATTGGC TGCTGAATGT TTATGACACG 
GGACGAACGG GGAGGATCCG GGTCCTGTCT TTTAAAACTG GCATCATTTC TCTGTGTAAA 
GCCCATTTGG AAGACAAGTA CAGATACCTC TTCAAGCAAG TGGCAAGTTC GACAGGATTT 
TGTGACCAGC GCAGGCTGGG CCTCCTCCTG CATGACTCTA TCCAGATCCC AAGACAGTTG 
GGTGAAGTCG CATCGTTCGG GGGCAGTAAC ATTGAGCCGA GTGTCAGGAG CTGCTTCCAG 
TTTGCTAATA ATAAGCCTGA GATCGAAGCG GCCCTCTTCC TAGACTGGAT GCGCCTGGAG 
CCCCAGTCCA TGGTGTGGCT GCCTGTCCTG CACCGAGTGG CTGCCGCGGA AACTGCCAAG 
CACCAGGCCA AGTGCAACAT CTGCAAGGAG TGTCCCATCA TCGGATTCAG GTACAGGAGT 
CTAAAGCACT TTAATTATGA CATCTGCCAA AGTTGCTTTT TTTCTGGTCG AGTTGCAAAA 
GGCCATAAAA TGCACTATCC CATGGTGGAA TACTGCACTC CGACTACATC GGGAGAAGAT 
GTCCGTGACT TTGCCAAGGT ACTAAAAAAC AAATTTCGAA CCAAAAGGTA TTTTGCGAAG 
CATCCCCGAA TGGGCTACCT GCCAGTGCAG ACTGTCTTAG AGGGGGACAA CATGGAAACT 
CCTGTCACTC TGATCAACTT CTGGCCGGTA GATTCTGCGC CTGCCTCGTC CCCTCAGCTT 
TCACACGATG ATACTCATTC ACGCATTGAG CATTATGCTA GCAGGCTAGC AGAAATGGAA 
AACAGCAATG GATCTTATCT AAATGATAGC ATCTCTCCTA ATGAGAGCAT AGATGATGAA 
CATTTGTTAA TCCAGCATTA CTGCCAAAGT TTGAACCAGG ACTCCCCCCT GAGCCAGCCT 
CGTAGTCCTG CCCAGATCTT GATTTCCTTA GAGAGTGAGG AAAGAGGGGA GCTAGAGAGA 
ATCCTAGCAG ATCTTGAGGA AGAAAACAGA AATCTGCAAG CAGAGTATGA TCGTCTAAAG 
CAGCAGCATG AACACAAAGG CCTGTCCCCA CTGCCATCCC CTCCTGAAAT GATGCCTACT 
TCTCCCCAAA GTCCCCGGGA TGCTGAGCTC ATCGCTGAGG CCAAGCTGCT GCGTCAACAC 
AAAGGCCGCC TGGAAGCCAG GATGCAAATC CTTGAAGACC ATAACAAACA ACTGGAATCC 
CAGTTACACA GGCTCAGGCA GCTGCTGGAG CAACCCCAGG CAGAGGCCAA GGTGAATGGT 
ACAACGGTGT CTTCTCCTTC TACCTCTCTT CAGAGGTCAG ATAGCAGTCA GCCTATGCTG 
CTCCGGGTGG TCGGCAGTCA GACTTCAGAA TCCATGGGCG AGGAAGACCT GCTCAGCCCT 
CCCCAGGACA CAAGCACAGG GTTAGAGGAA GTGATGGAGC AGCTCAACCA CTCCTTCCCT 
AGTTCCAGAG GAAGAAATAC CCCTGGGAAG CCAATGAGAG AGGACACAAT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013966825.1
CDS	131..1984
Translation	MREQLKGHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQVINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPVTLINFWPVDSAPASSPQLSHDDTHSRIEHYASRLAEMENSNGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKQQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDTSTGLEEVMEQLNHSFPSSRGRNTPGKPMREDTM

Target ORF information:

RefSeq Version	XM_014111350.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris dystrophin (DMD), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111350.1

ORF Insert Sequence:

ATGAGGGAGC AGCTCAAAGG CCACGAGACC CAAACAACTT GCTGGGACCA TCCCAAAATG 
ACAGAGCTCT ACCAGTCTTT AGCTGACCTG AATAATGTCA GGTTCTCAGC TTACAGGACT 
GCCATGAAAC TCCGAAGACT GCAGAAGGCC CTTTGCTTGG ATCTCTTGAG CCTATCGGCT 
GCATGCGATG CCTTGGACCA GCACAACCTC AAGCAAAATG ACCAGCCCAT GGATATCCTG 
CAGGTCATTA ACTGTCTGAC CACTATTTAT GATCGCCTAG AGCAAGAGCA CAACAATCTG 
GTCAACGTCC CTCTCTGCGT GGATATGTGT CTCAATTGGC TGCTGAATGT TTATGACACG 
GGACGAACGG GGAGGATCCG GGTCCTGTCT TTTAAAACTG GCATCATTTC TCTGTGTAAA 
GCCCATTTGG AAGACAAGTA CAGATACCTC TTCAAGCAAG TGGCAAGTTC GACAGGATTT 
TGTGACCAGC GCAGGCTGGG CCTCCTCCTG CATGACTCTA TCCAGATCCC AAGACAGTTG 
GGTGAAGTCG CATCGTTCGG GGGCAGTAAC ATTGAGCCGA GTGTCAGGAG CTGCTTCCAG 
TTTGCTAATA ATAAGCCTGA GATCGAAGCG GCCCTCTTCC TAGACTGGAT GCGCCTGGAG 
CCCCAGTCCA TGGTGTGGCT GCCTGTCCTG CACCGAGTGG CTGCCGCGGA AACTGCCAAG 
CACCAGGCCA AGTGCAACAT CTGCAAGGAG TGTCCCATCA TCGGATTCAG GTACAGGAGT 
CTAAAGCACT TTAATTATGA CATCTGCCAA AGTTGCTTTT TTTCTGGTCG AGTTGCAAAA 
GGCCATAAAA TGCACTATCC CATGGTGGAA TACTGCACTC CGACTACATC GGGAGAAGAT 
GTCCGTGACT TTGCCAAGGT ACTAAAAAAC AAATTTCGAA CCAAAAGGTA TTTTGCGAAG 
CATCCCCGAA TGGGCTACCT GCCAGTGCAG ACTGTCTTAG AGGGGGACAA CATGGAAACT 
CCTGTCACTC TGATCAACTT CTGGCCGGTA GATTCTGCGC CTGCCTCGTC CCCTCAGCTT 
TCACACGATG ATACTCATTC ACGCATTGAG CATTATGCTA GCAGGCTAGC AGAAATGGAA 
AACAGCAATG GATCTTATCT AAATGATAGC ATCTCTCCTA ATGAGAGCAT AGATGATGAA 
CATTTGTTAA TCCAGCATTA CTGCCAAAGT TTGAACCAGG ACTCCCCCCT GAGCCAGCCT 
CGTAGTCCTG CCCAGATCTT GATTTCCTTA GAGAGTGAGG AAAGAGGGGA GCTAGAGAGA 
ATCCTAGCAG ATCTTGAGGA AGAAAACAGA AATCTGCAAG CAGAGTATGA TCGTCTAAAG 
CAGCAGCATG AACACAAAGG CCTGTCCCCA CTGCCATCCC CTCCTGAAAT GATGCCTACT 
TCTCCCCAAA GTCCCCGGGA TGCTGAGCTC ATCGCTGAGG CCAAGCTGCT GCGTCAACAC 
AAAGGCCGCC TGGAAGCCAG GATGCAAATC CTTGAAGACC ATAACAAACA ACTGGAATCC 
CAGTTACACA GGCTCAGGCA GCTGCTGGAG CAACCCCAGG CAGAGGCCAA GGTGAATGGT 
ACAACGGTGT CTTCTCCTTC TACCTCTCTT CAGAGGTCAG ATAGCAGTCA GCCTATGCTG 
CTCCGGGTGG TCGGCAGTCA GACTTCAGAA TCCATGGGCG AGGAAGACCT GCTCAGCCCT 
CCCCAGGACA CAAGCACAGG GTTAGAGGAA GTGATGGAGC AGCTCAACCA CTCCTTCCCT 
AGTTCCAGAG GAAGAAATAC CCCTGGGAAG CCAATGAGAG AGGACACAAT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMD ( XM_014111351.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013966826.1 Canis lupus familiaris dystrophin (DMD), transcript variant X15, mRNA.

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CloneID	OCe45422
Clone ID Related Accession (Same CDS sequence)	XM_014111351.1 , XM_014111351.2
Accession Version	XM_014111351.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	dystrophin isoform X15
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGAGC AGCTCAAAGG CCACGAGACC CAAACAACTT GCTGGGACCA TCCCAAAATG 
ACAGAGCTCT ACCAGTCTTT AGCTGACCTG AATAATGTCA GGTTCTCAGC TTACAGGACT 
GCCATGAAAC TCCGAAGACT GCAGAAGGCC CTTTGCTTGG ATCTCTTGAG CCTATCGGCT 
GCATGCGATG CCTTGGACCA GCACAACCTC AAGCAAAATG ACCAGCCCAT GGATATCCTG 
CAGGTCATTA ACTGTCTGAC CACTATTTAT GATCGCCTAG AGCAAGAGCA CAACAATCTG 
GTCAACGTCC CTCTCTGCGT GGATATGTGT CTCAATTGGC TGCTGAATGT TTATGACACG 
GGACGAACGG GGAGGATCCG GGTCCTGTCT TTTAAAACTG GCATCATTTC TCTGTGTAAA 
GCCCATTTGG AAGACAAGTA CAGATACCTC TTCAAGCAAG TGGCAAGTTC GACAGGATTT 
TGTGACCAGC GCAGGCTGGG CCTCCTCCTG CATGACTCTA TCCAGATCCC AAGACAGTTG 
GGTGAAGTCG CATCGTTCGG GGGCAGTAAC ATTGAGCCGA GTGTCAGGAG CTGCTTCCAG 
TTTGCTAATA ATAAGCCTGA GATCGAAGCG GCCCTCTTCC TAGACTGGAT GCGCCTGGAG 
CCCCAGTCCA TGGTGTGGCT GCCTGTCCTG CACCGAGTGG CTGCCGCGGA AACTGCCAAG 
CACCAGGCCA AGTGCAACAT CTGCAAGGAG TGTCCCATCA TCGGATTCAG GTACAGGAGT 
CTAAAGCACT TTAATTATGA CATCTGCCAA AGTTGCTTTT TTTCTGGTCG AGTTGCAAAA 
GGCCATAAAA TGCACTATCC CATGGTGGAA TACTGCACTC CGACTACATC GGGAGAAGAT 
GTCCGTGACT TTGCCAAGGT ACTAAAAAAC AAATTTCGAA CCAAAAGGTA TTTTGCGAAG 
CATCCCCGAA TGGGCTACCT GCCAGTGCAG ACTGTCTTAG AGGGGGACAA CATGGAAACG 
CCTGCCTCGT CCCCTCAGCT TTCACACGAT GATACTCATT CACGCATTGA GCATTATGCT 
AGCAGGCTAG CAGAAATGGA AAACAGCAAT GGATCTTATC TAAATGATAG CATCTCTCCT 
AATGAGAGCA TAGATGATGA ACATTTGTTA ATCCAGCATT ACTGCCAAAG TTTGAACCAG 
GACTCCCCCC TGAGCCAGCC TCGTAGTCCT GCCCAGATCT TGATTTCCTT AGAGAGTGAG 
GAAAGAGGGG AGCTAGAGAG AATCCTAGCA GATCTTGAGG AAGAAAACAG AAATCTGCAA 
GCAGAGTATG ATCGTCTAAA GCAGCAGCAT GAACACAAAG GCCTGTCCCC ACTGCCATCC 
CCTCCTGAAA TGATGCCTAC TTCTCCCCAA AGTCCCCGGG ATGCTGAGCT CATCGCTGAG 
GCCAAGCTGC TGCGTCAACA CAAAGGCCGC CTGGAAGCCA GGATGCAAAT CCTTGAAGAC 
CATAACAAAC AACTGGAATC CCAGTTACAC AGGCTCAGGC AGCTGCTGGA GCAACCCCAG 
GCAGAGGCCA AGGTGAATGG TACAACGGTG TCTTCTCCTT CTACCTCTCT TCAGAGGTCA 
GATAGCAGTC AGCCTATGCT GCTCCGGGTG GTCGGCAGTC AGACTTCAGA ATCCATGGGC 
GAGGAAGACC TGCTCAGCCC TCCCCAGGAC ACAAGCACAG GGTTAGAGGA AGTGATGGAG 
CAGCTCAACC ACTCCTTCCC TAGTTCCAGA GGAAGAAATA CCCCTGGGAA GCCAATGAGA 
GAGGACACAA TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013966826.1
CDS	131..1945
Translation	MREQLKGHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQVINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPASSPQLSHDDTHSRIEHYASRLAEMENSNGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKQQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDTSTGLEEVMEQLNHSFPSSRGRNTPGKPMREDTM

Target ORF information:

RefSeq Version	XM_014111351.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris dystrophin (DMD), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111351.1

ORF Insert Sequence:

ATGAGGGAGC AGCTCAAAGG CCACGAGACC CAAACAACTT GCTGGGACCA TCCCAAAATG 
ACAGAGCTCT ACCAGTCTTT AGCTGACCTG AATAATGTCA GGTTCTCAGC TTACAGGACT 
GCCATGAAAC TCCGAAGACT GCAGAAGGCC CTTTGCTTGG ATCTCTTGAG CCTATCGGCT 
GCATGCGATG CCTTGGACCA GCACAACCTC AAGCAAAATG ACCAGCCCAT GGATATCCTG 
CAGGTCATTA ACTGTCTGAC CACTATTTAT GATCGCCTAG AGCAAGAGCA CAACAATCTG 
GTCAACGTCC CTCTCTGCGT GGATATGTGT CTCAATTGGC TGCTGAATGT TTATGACACG 
GGACGAACGG GGAGGATCCG GGTCCTGTCT TTTAAAACTG GCATCATTTC TCTGTGTAAA 
GCCCATTTGG AAGACAAGTA CAGATACCTC TTCAAGCAAG TGGCAAGTTC GACAGGATTT 
TGTGACCAGC GCAGGCTGGG CCTCCTCCTG CATGACTCTA TCCAGATCCC AAGACAGTTG 
GGTGAAGTCG CATCGTTCGG GGGCAGTAAC ATTGAGCCGA GTGTCAGGAG CTGCTTCCAG 
TTTGCTAATA ATAAGCCTGA GATCGAAGCG GCCCTCTTCC TAGACTGGAT GCGCCTGGAG 
CCCCAGTCCA TGGTGTGGCT GCCTGTCCTG CACCGAGTGG CTGCCGCGGA AACTGCCAAG 
CACCAGGCCA AGTGCAACAT CTGCAAGGAG TGTCCCATCA TCGGATTCAG GTACAGGAGT 
CTAAAGCACT TTAATTATGA CATCTGCCAA AGTTGCTTTT TTTCTGGTCG AGTTGCAAAA 
GGCCATAAAA TGCACTATCC CATGGTGGAA TACTGCACTC CGACTACATC GGGAGAAGAT 
GTCCGTGACT TTGCCAAGGT ACTAAAAAAC AAATTTCGAA CCAAAAGGTA TTTTGCGAAG 
CATCCCCGAA TGGGCTACCT GCCAGTGCAG ACTGTCTTAG AGGGGGACAA CATGGAAACG 
CCTGCCTCGT CCCCTCAGCT TTCACACGAT GATACTCATT CACGCATTGA GCATTATGCT 
AGCAGGCTAG CAGAAATGGA AAACAGCAAT GGATCTTATC TAAATGATAG CATCTCTCCT 
AATGAGAGCA TAGATGATGA ACATTTGTTA ATCCAGCATT ACTGCCAAAG TTTGAACCAG 
GACTCCCCCC TGAGCCAGCC TCGTAGTCCT GCCCAGATCT TGATTTCCTT AGAGAGTGAG 
GAAAGAGGGG AGCTAGAGAG AATCCTAGCA GATCTTGAGG AAGAAAACAG AAATCTGCAA 
GCAGAGTATG ATCGTCTAAA GCAGCAGCAT GAACACAAAG GCCTGTCCCC ACTGCCATCC 
CCTCCTGAAA TGATGCCTAC TTCTCCCCAA AGTCCCCGGG ATGCTGAGCT CATCGCTGAG 
GCCAAGCTGC TGCGTCAACA CAAAGGCCGC CTGGAAGCCA GGATGCAAAT CCTTGAAGAC 
CATAACAAAC AACTGGAATC CCAGTTACAC AGGCTCAGGC AGCTGCTGGA GCAACCCCAG 
GCAGAGGCCA AGGTGAATGG TACAACGGTG TCTTCTCCTT CTACCTCTCT TCAGAGGTCA 
GATAGCAGTC AGCCTATGCT GCTCCGGGTG GTCGGCAGTC AGACTTCAGA ATCCATGGGC 
GAGGAAGACC TGCTCAGCCC TCCCCAGGAC ACAAGCACAG GGTTAGAGGA AGTGATGGAG 
CAGCTCAACC ACTCCTTCCC TAGTTCCAGA GGAAGAAATA CCCCTGGGAA GCCAATGAGA 
GAGGACACAA TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMD ( XM_014111351.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013966826.1 Canis lupus familiaris dystrophin (DMD), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OCe45422
Clone ID Related Accession (Same CDS sequence)	XM_014111351.1 , XM_014111351.2
Accession Version	XM_014111351.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	dystrophin isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014111351.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGAGC AGCTCAAAGG CCACGAGACC CAAACAACTT GCTGGGACCA TCCCAAAATG 
ACAGAGCTCT ACCAGTCTTT AGCTGACCTG AATAATGTCA GGTTCTCAGC TTACAGGACT 
GCCATGAAAC TCCGAAGACT GCAGAAGGCC CTTTGCTTGG ATCTCTTGAG CCTATCGGCT 
GCATGCGATG CCTTGGACCA GCACAACCTC AAGCAAAATG ACCAGCCCAT GGATATCCTG 
CAGGTCATTA ACTGTCTGAC CACTATTTAT GATCGCCTAG AGCAAGAGCA CAACAATCTG 
GTCAACGTCC CTCTCTGCGT GGATATGTGT CTCAATTGGC TGCTGAATGT TTATGACACG 
GGACGAACGG GGAGGATCCG GGTCCTGTCT TTTAAAACTG GCATCATTTC TCTGTGTAAA 
GCCCATTTGG AAGACAAGTA CAGATACCTC TTCAAGCAAG TGGCAAGTTC GACAGGATTT 
TGTGACCAGC GCAGGCTGGG CCTCCTCCTG CATGACTCTA TCCAGATCCC AAGACAGTTG 
GGTGAAGTCG CATCGTTCGG GGGCAGTAAC ATTGAGCCGA GTGTCAGGAG CTGCTTCCAG 
TTTGCTAATA ATAAGCCTGA GATCGAAGCG GCCCTCTTCC TAGACTGGAT GCGCCTGGAG 
CCCCAGTCCA TGGTGTGGCT GCCTGTCCTG CACCGAGTGG CTGCCGCGGA AACTGCCAAG 
CACCAGGCCA AGTGCAACAT CTGCAAGGAG TGTCCCATCA TCGGATTCAG GTACAGGAGT 
CTAAAGCACT TTAATTATGA CATCTGCCAA AGTTGCTTTT TTTCTGGTCG AGTTGCAAAA 
GGCCATAAAA TGCACTATCC CATGGTGGAA TACTGCACTC CGACTACATC GGGAGAAGAT 
GTCCGTGACT TTGCCAAGGT ACTAAAAAAC AAATTTCGAA CCAAAAGGTA TTTTGCGAAG 
CATCCCCGAA TGGGCTACCT GCCAGTGCAG ACTGTCTTAG AGGGGGACAA CATGGAAACG 
CCTGCCTCGT CCCCTCAGCT TTCACACGAT GATACTCATT CACGCATTGA GCATTATGCT 
AGCAGGCTAG CAGAAATGGA AAACAGCAAT GGATCTTATC TAAATGATAG CATCTCTCCT 
AATGAGAGCA TAGATGATGA ACATTTGTTA ATCCAGCATT ACTGCCAAAG TTTGAACCAG 
GACTCCCCCC TGAGCCAGCC TCGTAGTCCT GCCCAGATCT TGATTTCCTT AGAGAGTGAG 
GAAAGAGGGG AGCTAGAGAG AATCCTAGCA GATCTTGAGG AAGAAAACAG AAATCTGCAA 
GCAGAGTATG ATCGTCTAAA GCAGCAGCAT GAACACAAAG GCCTGTCCCC ACTGCCATCC 
CCTCCTGAAA TGATGCCTAC TTCTCCCCAA AGTCCCCGGG ATGCTGAGCT CATCGCTGAG 
GCCAAGCTGC TGCGTCAACA CAAAGGCCGC CTGGAAGCCA GGATGCAAAT CCTTGAAGAC 
CATAACAAAC AACTGGAATC CCAGTTACAC AGGCTCAGGC AGCTGCTGGA GCAACCCCAG 
GCAGAGGCCA AGGTGAATGG TACAACGGTG TCTTCTCCTT CTACCTCTCT TCAGAGGTCA 
GATAGCAGTC AGCCTATGCT GCTCCGGGTG GTCGGCAGTC AGACTTCAGA ATCCATGGGC 
GAGGAAGACC TGCTCAGCCC TCCCCAGGAC ACAAGCACAG GGTTAGAGGA AGTGATGGAG 
CAGCTCAACC ACTCCTTCCC TAGTTCCAGA GGAAGAAATA CCCCTGGGAA GCCAATGAGA 
GAGGACACAA TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013966826.1
CDS	135..1949
Translation	MREQLKGHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQVINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPASSPQLSHDDTHSRIEHYASRLAEMENSNGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKQQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDTSTGLEEVMEQLNHSFPSSRGRNTPGKPMREDTM

Target ORF information:

RefSeq Version	XM_014111351.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris dystrophin (DMD), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111351.2

ORF Insert Sequence:

ATGAGGGAGC AGCTCAAAGG CCACGAGACC CAAACAACTT GCTGGGACCA TCCCAAAATG 
ACAGAGCTCT ACCAGTCTTT AGCTGACCTG AATAATGTCA GGTTCTCAGC TTACAGGACT 
GCCATGAAAC TCCGAAGACT GCAGAAGGCC CTTTGCTTGG ATCTCTTGAG CCTATCGGCT 
GCATGCGATG CCTTGGACCA GCACAACCTC AAGCAAAATG ACCAGCCCAT GGATATCCTG 
CAGGTCATTA ACTGTCTGAC CACTATTTAT GATCGCCTAG AGCAAGAGCA CAACAATCTG 
GTCAACGTCC CTCTCTGCGT GGATATGTGT CTCAATTGGC TGCTGAATGT TTATGACACG 
GGACGAACGG GGAGGATCCG GGTCCTGTCT TTTAAAACTG GCATCATTTC TCTGTGTAAA 
GCCCATTTGG AAGACAAGTA CAGATACCTC TTCAAGCAAG TGGCAAGTTC GACAGGATTT 
TGTGACCAGC GCAGGCTGGG CCTCCTCCTG CATGACTCTA TCCAGATCCC AAGACAGTTG 
GGTGAAGTCG CATCGTTCGG GGGCAGTAAC ATTGAGCCGA GTGTCAGGAG CTGCTTCCAG 
TTTGCTAATA ATAAGCCTGA GATCGAAGCG GCCCTCTTCC TAGACTGGAT GCGCCTGGAG 
CCCCAGTCCA TGGTGTGGCT GCCTGTCCTG CACCGAGTGG CTGCCGCGGA AACTGCCAAG 
CACCAGGCCA AGTGCAACAT CTGCAAGGAG TGTCCCATCA TCGGATTCAG GTACAGGAGT 
CTAAAGCACT TTAATTATGA CATCTGCCAA AGTTGCTTTT TTTCTGGTCG AGTTGCAAAA 
GGCCATAAAA TGCACTATCC CATGGTGGAA TACTGCACTC CGACTACATC GGGAGAAGAT 
GTCCGTGACT TTGCCAAGGT ACTAAAAAAC AAATTTCGAA CCAAAAGGTA TTTTGCGAAG 
CATCCCCGAA TGGGCTACCT GCCAGTGCAG ACTGTCTTAG AGGGGGACAA CATGGAAACG 
CCTGCCTCGT CCCCTCAGCT TTCACACGAT GATACTCATT CACGCATTGA GCATTATGCT 
AGCAGGCTAG CAGAAATGGA AAACAGCAAT GGATCTTATC TAAATGATAG CATCTCTCCT 
AATGAGAGCA TAGATGATGA ACATTTGTTA ATCCAGCATT ACTGCCAAAG TTTGAACCAG 
GACTCCCCCC TGAGCCAGCC TCGTAGTCCT GCCCAGATCT TGATTTCCTT AGAGAGTGAG 
GAAAGAGGGG AGCTAGAGAG AATCCTAGCA GATCTTGAGG AAGAAAACAG AAATCTGCAA 
GCAGAGTATG ATCGTCTAAA GCAGCAGCAT GAACACAAAG GCCTGTCCCC ACTGCCATCC 
CCTCCTGAAA TGATGCCTAC TTCTCCCCAA AGTCCCCGGG ATGCTGAGCT CATCGCTGAG 
GCCAAGCTGC TGCGTCAACA CAAAGGCCGC CTGGAAGCCA GGATGCAAAT CCTTGAAGAC 
CATAACAAAC AACTGGAATC CCAGTTACAC AGGCTCAGGC AGCTGCTGGA GCAACCCCAG 
GCAGAGGCCA AGGTGAATGG TACAACGGTG TCTTCTCCTT CTACCTCTCT TCAGAGGTCA 
GATAGCAGTC AGCCTATGCT GCTCCGGGTG GTCGGCAGTC AGACTTCAGA ATCCATGGGC 
GAGGAAGACC TGCTCAGCCC TCCCCAGGAC ACAAGCACAG GGTTAGAGGA AGTGATGGAG 
CAGCTCAACC ACTCCTTCCC TAGTTCCAGA GGAAGAAATA CCCCTGGGAA GCCAATGAGA 
GAGGACACAA TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMD ( XM_022415520.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022271228.1 Canis lupus familiaris dystrophin (DMD), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OCe285079
Clone ID Related Accession (Same CDS sequence)	XM_022415520.1
Accession Version	XM_022415520.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	dystrophin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCATCTT CATTGCTGTT GGAGGTACCT GCACTGGCAG ATTTCAACCG AGCTTGGACA 
GAACTTACCG ACTGGCTGTC TCTGCTTGAT CGAGTTATAA AATCACAGAG GGTGATGGTG 
GGTGATCTTG AAGACATTAA CGAGATGATC ATCAAGCAGA AGGCAACGCT GCAGGATTTG 
GAACAGAGGC GCCCCCAGTT GGAAGAACTC ATTACCGCTG CCCAGAATTT GAAAAACAAG 
ACCAGCAATC AAGAGGCTAG AACAATCATT ACTGATCGAA TTGAAAGAAT TCAGAGTCAG 
TGGGATGAAG TACAGGAACA TCTTCAGAAC CGGAGGCAAC AGTTGAATGA AATGTTAAAG 
GATTCCACAC AATGGCTGGA AGCTAAAGAG GAGGCTGAGC AGGTGTTGGG GCAGGCCAGA 
GCCAAGCTTG AGTCATGGAA GGAGGCTCCC TACACAGTAG ATGCAATCCA AAAGAAAATC 
ACAGAAACCA AGCAGTTGGC CAAAGACCTC CGCCAGTGGC AGATAAATGT AGATGTTGCA 
AATGATTTGG CACTGAAACT TCTCCGAGAT TATTCTGCAG ATGATACCAG AAAAGTACAC 
ATGATAACAG AGAACATCAA TGCCTCTTGG GCAAGCATCC ATAAAAGATT GAGTGAGCGA 
GAGGCTGCTC TGGAAGAAAC CCACAGATTA CTGCAACAGT TCCCCTTGGA CCTGGAGAAG 
TTCCTTGCCT GGCTTACAGA AGCCGAAACA ACTGCCAACG TCCTGCAGGA TGCCACCCAT 
AAGGAAAGGC TTCTAGAAGA TTCCAAGGGA GTAAGAGAGC TGATGAAACA ATGGCAAGAT 
CTCCAAGGAG AAATCGAAGC TCACACAGAT ATCTATCACA ACCTGGACGA AAATGGCCAA 
AAAGTCCTGA GATCCCTGGA AGGTTCTGAC GATGCAGCCT TGTTGCAAAG ACGTTTGGAT 
AACATGAACT TCAAGTGGAG CGAACTTCGG AAAAAGTCTC TCAACATTAG GTCTCACTTG 
GAAGCCAGTT CTGACCAGTG GAAGCGTCTG CACCTTTCTC TTCAGGAACT TCTGGTATGG 
CTCCAGCTGA AAGATGATGA GTTAAGCCGG CAGGCACCCA TTGGAGGAGA CTTTCCAGCG 
GTGCAGAAGC AGAATGATGT ACACAGGGCC TTCAAGAGGG AATTGAAAAC GAAAGAACCT 
GTAATCATGA GTACTCTTGA GACTGTACGA ATATTTCTGA CAGAGCAGCC TTTAGAAGGA 
CTAGAGAAAC TCTACCAGGA GCCCAGAGAG CTGCCTCCTG AAGAGAGAGC CCAGAATGTC 
ACACGGCTCC TACGAAAGCA AGCTGAGGAG GTCAACACTC AGTGGGAAAA ACTGAACGTG 
CACTCTGCAG ACTGGCAGAG AAAAATAGAC GAGGCCCTCG AAAGACTCCA GGAGCTTCAG 
GAAGCAACAG ATGAGCTGGA TCTCAAACTA CGTCAGGCAG AGGTGATCAA AGGATCCTGG 
CAGCCTGTGG GTGATCTCCT CATTGACTCT CTCCAAGATC ACCTCGAAAA AGTCAAGGCG 
CTTCGAGGAG AAATTACACC TCTGAAAGAG AATGTCAGCT ACGTCAATGA CCTTGCTCGC 
CAACTCACTA CGTTGGGCAT TCAGCTGTCA CCATATAACC TCAACACTCT GGAAGACCTG 
AACACCAGAT GGAAGCTTCT GCAGGTGGCC ATTGAGGACC GCATCAGGCA GCTGCATGAA 
GCGCACAGGG ACTTTGGACC AGCCTCCCAG CACTTCCTTT CCACTTCTGT CCAGGGTCCC 
TGGGAGAGAG CCATCTCACC AAACAAAGTG CCCTACTATA TCAACCACGA GACCCAAACA 
ACTTGCTGGG ACCATCCCAA AATGACAGAG CTCTACCAGT CTTTAGCTGA CCTGAATAAT 
GTCAGGTTCT CAGCTTACAG GACTGCCATG AAACTCCGAA GACTGCAGAA GGCCCTTTGC 
TTGGATCTCT TGAGCCTATC GGCTGCATGC GATGCCTTGG ACCAGCACAA CCTCAAGCAA 
AATGACCAGC CCATGGATAT CCTGCAGGTC ATTAACTGTC TGACCACTAT TTATGATCGC 
CTAGAGCAAG AGCACAACAA TCTGGTCAAC GTCCCTCTCT GCGTGGATAT GTGTCTCAAT 
TGGCTGCTGA ATGTTTATGA CACGGGACGA ACGGGGAGGA TCCGGGTCCT GTCTTTTAAA 
ACTGGCATCA TTTCTCTGTG TAAAGCCCAT TTGGAAGACA AGTACAGATA CCTCTTCAAG 
CAAGTGGCAA GTTCGACAGG ATTTTGTGAC CAGCGCAGGC TGGGCCTCCT CCTGCATGAC 
TCTATCCAGA TCCCAAGACA GTTGGGTGAA GTCGCATCGT TCGGGGGCAG TAACATTGAG 
CCGAGTGTCA GGAGCTGCTT CCAGTTTGCT AATAATAAGC CTGAGATCGA AGCGGCCCTC 
TTCCTAGACT GGATGCGCCT GGAGCCCCAG TCCATGGTGT GGCTGCCTGT CCTGCACCGA 
GTGGCTGCCG CGGAAACTGC CAAGCACCAG GCCAAGTGCA ACATCTGCAA GGAGTGTCCC 
ATCATCGGAT TCAGGTACAG GAGTCTAAAG CACTTTAATT ATGACATCTG CCAAAGTTGC 
TTTTTTTCTG GTCGAGTTGC AAAAGGCCAT AAAATGCACT ATCCCATGGT GGAATACTGC 
ACTCCGACTA CATCGGGAGA AGATGTCCGT GACTTTGCCA AGGTACTAAA AAACAAATTT 
CGAACCAAAA GGTATTTTGC GAAGCATCCC CGAATGGGCT ACCTGCCAGT GCAGACTGTC 
TTAGAGGGGG ACAACATGGA AACTCCTGTC ACTCTGATCA ACTTCTGGCC GGTAGATTCT 
GCGCCTGCCT CGTCCCCTCA GCTTTCACAC GATGATACTC ATTCACGCAT TGAGCATTAT 
GCTAGCAGGC TAGCAGAAAT GGAAAACAGC AATGGATCTT ATCTAAATGA TAGCATCTCT 
CCTAATGAGA GCATAGATGA TGAACATTTG TTAATCCAGC ATTACTGCCA AAGTTTGAAC 
CAGGACTCCC CCCTGAGCCA GCCTCGTAGT CCTGCCCAGA TCTTGATTTC CTTAGAGAGT 
GAGGAAAGAG GGGAGCTAGA GAGAATCCTA GCAGATCTTG AGGAAGAAAA CAGAAATCTG 
CAAGCAGAGT ATGATCGTCT AAAGCAGCAG CATGAACACA AAGGCCTGTC CCCACTGCCA 
TCCCCTCCTG AAATGATGCC TACTTCTCCC CAAAGTCCCC GGGATGCTGA GCTCATCGCT 
GAGGCCAAGC TGCTGCGTCA ACACAAAGGC CGCCTGGAAG CCAGGATGCA AATCCTTGAA 
GACCATAACA AACAACTGGA ATCCCAGTTA CACAGGCTCA GGCAGCTGCT GGAGCAACCC 
CAGGCAGAGG CCAAGGTGAA TGGTACAACG GTGTCTTCTC CTTCTACCTC TCTTCAGAGG 
TCAGATAGCA GTCAGCCTAT GCTGCTCCGG GTGGTCGGCA GTCAGACTTC AGAATCCATG 
GGCGAGGAAG ACCTGCTCAG CCCTCCCCAG GACACAAGCA CAGGGTTAGA GGAAGTGATG 
GAGCAGCTCA ACCACTCCTT CCCTAGTTCC AGAGGACACA ATGTAGGAAG CCTTTTCCAC 
TTGGCAGATG ATTTGGGCAG AGCGATGGAG TCCTTAGTTT CAGTCATGAC AGATGAAGAA 
GGGGCAGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022271228.1
CDS	233..3964
Translation	MPSSLLLEVPALADFNRAWTELTDWLSLLDRVIKSQRVMVGDLEDINEMIIKQKATLQDLEQRRPQLEELITAAQNLKNKTSNQEARTIITDRIERIQSQWDEVQEHLQNRRQQLNEMLKDSTQWLEAKEEAEQVLGQARAKLESWKEAPYTVDAIQKKITETKQLAKDLRQWQINVDVANDLALKLLRDYSADDTRKVHMITENINASWASIHKRLSEREAALEETHRLLQQFPLDLEKFLAWLTEAETTANVLQDATHKERLLEDSKGVRELMKQWQDLQGEIEAHTDIYHNLDENGQKVLRSLEGSDDAALLQRRLDNMNFKWSELRKKSLNIRSHLEASSDQWKRLHLSLQELLVWLQLKDDELSRQAPIGGDFPAVQKQNDVHRAFKRELKTKEPVIMSTLETVRIFLTEQPLEGLEKLYQEPRELPPEERAQNVTRLLRKQAEEVNTQWEKLNVHSADWQRKIDEALERLQELQEATDELDLKLRQAEVIKGSWQPVGDLLIDSLQDHLEKVKALRGEITPLKENVSYVNDLARQLTTLGIQLSPYNLNTLEDLNTRWKLLQVAIEDRIRQLHEAHRDFGPASQHFLSTSVQGPWERAISPNKVPYYINHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQVINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPVTLINFWPVDSAPASSPQLSHDDTHSRIEHYASRLAEMENSNGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKQQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDTSTGLEEVMEQLNHSFPSSRGHNVGSLFHLADDLGRAMESLVSVMTDEEGAE

Target ORF information:

RefSeq Version	XM_022415520.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris dystrophin (DMD), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022415520.1

ORF Insert Sequence:

ATGCCATCTT CATTGCTGTT GGAGGTACCT GCACTGGCAG ATTTCAACCG AGCTTGGACA 
GAACTTACCG ACTGGCTGTC TCTGCTTGAT CGAGTTATAA AATCACAGAG GGTGATGGTG 
GGTGATCTTG AAGACATTAA CGAGATGATC ATCAAGCAGA AGGCAACGCT GCAGGATTTG 
GAACAGAGGC GCCCCCAGTT GGAAGAACTC ATTACCGCTG CCCAGAATTT GAAAAACAAG 
ACCAGCAATC AAGAGGCTAG AACAATCATT ACTGATCGAA TTGAAAGAAT TCAGAGTCAG 
TGGGATGAAG TACAGGAACA TCTTCAGAAC CGGAGGCAAC AGTTGAATGA AATGTTAAAG 
GATTCCACAC AATGGCTGGA AGCTAAAGAG GAGGCTGAGC AGGTGTTGGG GCAGGCCAGA 
GCCAAGCTTG AGTCATGGAA GGAGGCTCCC TACACAGTAG ATGCAATCCA AAAGAAAATC 
ACAGAAACCA AGCAGTTGGC CAAAGACCTC CGCCAGTGGC AGATAAATGT AGATGTTGCA 
AATGATTTGG CACTGAAACT TCTCCGAGAT TATTCTGCAG ATGATACCAG AAAAGTACAC 
ATGATAACAG AGAACATCAA TGCCTCTTGG GCAAGCATCC ATAAAAGATT GAGTGAGCGA 
GAGGCTGCTC TGGAAGAAAC CCACAGATTA CTGCAACAGT TCCCCTTGGA CCTGGAGAAG 
TTCCTTGCCT GGCTTACAGA AGCCGAAACA ACTGCCAACG TCCTGCAGGA TGCCACCCAT 
AAGGAAAGGC TTCTAGAAGA TTCCAAGGGA GTAAGAGAGC TGATGAAACA ATGGCAAGAT 
CTCCAAGGAG AAATCGAAGC TCACACAGAT ATCTATCACA ACCTGGACGA AAATGGCCAA 
AAAGTCCTGA GATCCCTGGA AGGTTCTGAC GATGCAGCCT TGTTGCAAAG ACGTTTGGAT 
AACATGAACT TCAAGTGGAG CGAACTTCGG AAAAAGTCTC TCAACATTAG GTCTCACTTG 
GAAGCCAGTT CTGACCAGTG GAAGCGTCTG CACCTTTCTC TTCAGGAACT TCTGGTATGG 
CTCCAGCTGA AAGATGATGA GTTAAGCCGG CAGGCACCCA TTGGAGGAGA CTTTCCAGCG 
GTGCAGAAGC AGAATGATGT ACACAGGGCC TTCAAGAGGG AATTGAAAAC GAAAGAACCT 
GTAATCATGA GTACTCTTGA GACTGTACGA ATATTTCTGA CAGAGCAGCC TTTAGAAGGA 
CTAGAGAAAC TCTACCAGGA GCCCAGAGAG CTGCCTCCTG AAGAGAGAGC CCAGAATGTC 
ACACGGCTCC TACGAAAGCA AGCTGAGGAG GTCAACACTC AGTGGGAAAA ACTGAACGTG 
CACTCTGCAG ACTGGCAGAG AAAAATAGAC GAGGCCCTCG AAAGACTCCA GGAGCTTCAG 
GAAGCAACAG ATGAGCTGGA TCTCAAACTA CGTCAGGCAG AGGTGATCAA AGGATCCTGG 
CAGCCTGTGG GTGATCTCCT CATTGACTCT CTCCAAGATC ACCTCGAAAA AGTCAAGGCG 
CTTCGAGGAG AAATTACACC TCTGAAAGAG AATGTCAGCT ACGTCAATGA CCTTGCTCGC 
CAACTCACTA CGTTGGGCAT TCAGCTGTCA CCATATAACC TCAACACTCT GGAAGACCTG 
AACACCAGAT GGAAGCTTCT GCAGGTGGCC ATTGAGGACC GCATCAGGCA GCTGCATGAA 
GCGCACAGGG ACTTTGGACC AGCCTCCCAG CACTTCCTTT CCACTTCTGT CCAGGGTCCC 
TGGGAGAGAG CCATCTCACC AAACAAAGTG CCCTACTATA TCAACCACGA GACCCAAACA 
ACTTGCTGGG ACCATCCCAA AATGACAGAG CTCTACCAGT CTTTAGCTGA CCTGAATAAT 
GTCAGGTTCT CAGCTTACAG GACTGCCATG AAACTCCGAA GACTGCAGAA GGCCCTTTGC 
TTGGATCTCT TGAGCCTATC GGCTGCATGC GATGCCTTGG ACCAGCACAA CCTCAAGCAA 
AATGACCAGC CCATGGATAT CCTGCAGGTC ATTAACTGTC TGACCACTAT TTATGATCGC 
CTAGAGCAAG AGCACAACAA TCTGGTCAAC GTCCCTCTCT GCGTGGATAT GTGTCTCAAT 
TGGCTGCTGA ATGTTTATGA CACGGGACGA ACGGGGAGGA TCCGGGTCCT GTCTTTTAAA 
ACTGGCATCA TTTCTCTGTG TAAAGCCCAT TTGGAAGACA AGTACAGATA CCTCTTCAAG 
CAAGTGGCAA GTTCGACAGG ATTTTGTGAC CAGCGCAGGC TGGGCCTCCT CCTGCATGAC 
TCTATCCAGA TCCCAAGACA GTTGGGTGAA GTCGCATCGT TCGGGGGCAG TAACATTGAG 
CCGAGTGTCA GGAGCTGCTT CCAGTTTGCT AATAATAAGC CTGAGATCGA AGCGGCCCTC 
TTCCTAGACT GGATGCGCCT GGAGCCCCAG TCCATGGTGT GGCTGCCTGT CCTGCACCGA 
GTGGCTGCCG CGGAAACTGC CAAGCACCAG GCCAAGTGCA ACATCTGCAA GGAGTGTCCC 
ATCATCGGAT TCAGGTACAG GAGTCTAAAG CACTTTAATT ATGACATCTG CCAAAGTTGC 
TTTTTTTCTG GTCGAGTTGC AAAAGGCCAT AAAATGCACT ATCCCATGGT GGAATACTGC 
ACTCCGACTA CATCGGGAGA AGATGTCCGT GACTTTGCCA AGGTACTAAA AAACAAATTT 
CGAACCAAAA GGTATTTTGC GAAGCATCCC CGAATGGGCT ACCTGCCAGT GCAGACTGTC 
TTAGAGGGGG ACAACATGGA AACTCCTGTC ACTCTGATCA ACTTCTGGCC GGTAGATTCT 
GCGCCTGCCT CGTCCCCTCA GCTTTCACAC GATGATACTC ATTCACGCAT TGAGCATTAT 
GCTAGCAGGC TAGCAGAAAT GGAAAACAGC AATGGATCTT ATCTAAATGA TAGCATCTCT 
CCTAATGAGA GCATAGATGA TGAACATTTG TTAATCCAGC ATTACTGCCA AAGTTTGAAC 
CAGGACTCCC CCCTGAGCCA GCCTCGTAGT CCTGCCCAGA TCTTGATTTC CTTAGAGAGT 
GAGGAAAGAG GGGAGCTAGA GAGAATCCTA GCAGATCTTG AGGAAGAAAA CAGAAATCTG 
CAAGCAGAGT ATGATCGTCT AAAGCAGCAG CATGAACACA AAGGCCTGTC CCCACTGCCA 
TCCCCTCCTG AAATGATGCC TACTTCTCCC CAAAGTCCCC GGGATGCTGA GCTCATCGCT 
GAGGCCAAGC TGCTGCGTCA ACACAAAGGC CGCCTGGAAG CCAGGATGCA AATCCTTGAA 
GACCATAACA AACAACTGGA ATCCCAGTTA CACAGGCTCA GGCAGCTGCT GGAGCAACCC 
CAGGCAGAGG CCAAGGTGAA TGGTACAACG GTGTCTTCTC CTTCTACCTC TCTTCAGAGG 
TCAGATAGCA GTCAGCCTAT GCTGCTCCGG GTGGTCGGCA GTCAGACTTC AGAATCCATG 
GGCGAGGAAG ACCTGCTCAG CCCTCCCCAG GACACAAGCA CAGGGTTAGA GGAAGTGATG 
GAGCAGCTCA ACCACTCCTT CCCTAGTTCC AGAGGACACA ATGTAGGAAG CCTTTTCCAC 
TTGGCAGATG ATTTGGGCAG AGCGATGGAG TCCTTAGTTT CAGTCATGAC AGATGAAGAA 
GGGGCAGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMD ( NM_001003343.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001003343.1 Canis lupus familiaris dystrophin (DMD), mRNA.

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CloneID	OCe00364
Clone ID Related Accession (Same CDS sequence)	NM_001003343.1
Accession Version	NM_001003343.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Canis lupus familiaris(dog)
Product	dystrophin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF070485.1. On Jan 18, 2005 this sequence version replaced XM_537990.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF070485.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA103936001, SAMEA103936002 [ECO:0000350] ##Evidence-Data-END##

ATGCTTTGGT GGGAAGAAGT AGAGGACTGT TATGAAAGAG AAGATGTTCA AAAGAAAACA 
TTCACAAAAT GGGTAAATGC ACAGTTTTCT AAGTTTGGGA AGCAGCACAT AGAGAACCTC 
TTCAGTGACC TACAGGATGG GAGACGCCTC CTAGACCTTT TGGAAGGCCT GACAGGGCAA 
AAACTGCCAA AAGAAAAAGG ATCCACAAGA GTTCATGCCC TGAACAATGT CAACAAGGCA 
CTGCGCGTCT TGCAGAAAAA TAATGTTGAT TTAGTGAACA TTGGAAGTAC TGACATAGTA 
GATGGAAATC ACAAACTGAC TCTTGGTTTG ATTTGGAATA TAATCCTCCA CTGGCAGGTC 
AAAAATGTAA TGAAAAATAT CATGGCTGGA TTGCAACAAA CCAACAGTGA AAAGATTCTC 
CTGAGCTGGG TCCGACAATC AACTCGTAAT TATCCACAGG TTAATGTCAT TAACTTCACC 
ACCAGCTGGT CTGATGGCCT GGCTTTGAAC GCTCTCATCC ACAGTCATAG GCCAGACCTG 
TTTGATTGGA ATAGTGTGGT TTGCCAGCAG TCAGCCACAC AACGCCTGGA ACATGCATTC 
AACATTGCCA AATATCAATT AGGCATAGAG AAACTGCTTG ATCCTGAAGA TGTTGCCACC 
ACTTATCCAG ATAAGAAGTC CATCTTAATG TATATCACAT CACTCTTCCA AGTTTTGCCT 
CAACAAGTGA GCATTGAAGC CATCCAGGAA GTGGAAATGT TGCCAAGGCC ATCTCAAGTT 
ACTAGAGAAG AACATTTTCA GATACATCAT CAAATGCACT ATTCTCAACA GATCACAGTC 
AGTCTAGCAC AGGGATATGA ACGAGCCCCT TCCTTTCCTA AGCCTCGGTT CAAGAGCTAT 
GCCTACACAC AGGCTGCTTA TGTCACCACT TCTGACCCCA CACGGAGCCC ACTTCCTTCA 
CAGCATTTGG AAACTCCTGA AGACAAGTCA TTTGGCCGGT CATTGACAGA GACCGAAGCA 
AACCTGGACA GTTATCAAAC AGCTTTGGAA GAAGTACTCT CGTGGCTTCT TTCAGCTGAG 
GATGCACTGC AAGCCCAAGG AGAGATTTCT AATGATGTCG AAGAAGTGAA AGAACAATTT 
CATACTCATG AGGGATATAT GATGGACTTG ACATCCCATC AGGGACGGGT CGGTAATGTT 
CTCCAACTGG GAAGTCAACT GATTGGAACA GGGAAATTAT CAGAAGATGA AGAAACCGAA 
GTGCAGGAAC AAATGAATCT CCTCAATTCA AGATGGGAAT GCCTCAGGGT AGCTAGCATG 
GAAAAACAAA GCAATTTACA TAAAGTTCTA ATGGATCTCC AGAATCAGCA ACTGAAAGAG 
TTAAATGACT GGCTAACCAA AACAGAAGAG AGAACAAGGA AAATGGAGAA GGAGCCCCTT 
GGACCTGATA TTGAAGACCT AAAACGCCAA GTACAACAAC ATAAGGTGCT TCAAGAAGAC 
TTAGAACAGG AACAAGTCAG GGTCAATTCC CTCACTCATA TGGTGGTGGT AGTCGATGAA 
TCTAGTGGAG ACCATGCAAC TGCTGCTTTG GAAGAACAAC TTAAGGTACT GGGAGATCGA 
TGGGCAAACA TCTGTAGGTG GACAGAAGAT CGCTGGGTTC TTTTACAAGA CATCCTCCTA 
AAATGGCAGC GTTTTACTGA AGAACAGTGC CTTTTTAGTG CATGGCTTTC GGAGAAGGAA 
GATGCAGTGA ACAAGATTCA CACAACTGGC TTTAAGGATC AAAGTGAAGT GTTATCAAAT 
CTTCAGAAAC TGGCTGTCTT AAAAACAGAT CTGGAAAAGA AGAAGCAAAC CATGGACAAA 
CTCTGCTCAC TCAACCAAGA CCTTCTTTCA GCGCTGAAAA ACACAGTGGT AGCCCACAAG 
ATGGAAGCAT GGCTGGACAA CTTTGCCCAG CGCTGGGATA ATTTAGTCCA GAAACTTGAA 
AAAAGTTCAG CACAGATTTC ACAGGCTGTC ACCACCACTC AGCCATCACT AACACAGACA 
ACTGTAATGG AAACAGTAAC TATGGTGACC ACGAGGGAAC ACATCTTGGT AAAGCATGCC 
CAAGAGGAAC TGCCACCACC ACCCCCTCAG AAGAAGAGGC AGATTATCGT GGATTCTGAA 
ATTAGGAAAA GGTTGGATGT CGATATAACT GAACTTCACA GTTGGATTAC TCGTTCAGAA 
GCTGTGTTGC AGAGTCCTGA ATTTGCAATC TATCGGAAGG AAGGCAACTT CTCAGACCTT 
AAAGAAAAAG TCAATGCCAT AGAGCGAGAA AAAGCCGAGA AGTTCAGAAA ACTGCAAGAT 
GCCAGCAGAT CAGCTCAGGC CCTGGTGGAA CAGATGGTGA ATGAGGGTGT TAATGCTGAC 
AGCATCAAAC AAGCCTCCGA ACAACTGAAC AGCCGGTGGA TAGAGTTCTG CCAATTGCTA 
AGCGAGAGAC TTAACTGGCT GGAGTATCAG AACAACATCA TCACTTTCTA TAATCAGCTA 
CAACAATTGG AGCAGATGAC AACTACTGCT GAAAACTGGT TGAAAACCCA GCCTACCACC 
ACATCAGAGC CAACAGCAAT TAAAAGCCAG TTAAAAATTT GTAAGGATGA AATCAACCGA 
CTGTCAGCTC TTCAGCCTCA AATCGAGCGA TTAAAAATTC AAAGCATAGC CCTGAAAGAG 
AAAGGACAAG GGCCAATGTT CCTGGATGCA GACTTTGTGG CCTTTACAAA TCATTTTAAC 
CAAGTCTTTG CTGATGTGCA GGCAAGAGAA AAAGAGCTAC AAACAATTTT TGACAGTTTG 
CCACCCATGC GCTATCAGGA GACTATGAGT ACCATCCTGA CATGGATCCA GCAGTCAGAA 
ACCAAACTCT CTATACCTCA GGTTACTGTC ACTGAATATG ACATCATGGA ACAGAGACTC 
GGAGAGCTAC AGGCTTTACA AAGCTCTCTG CAAGAGCAAC AAAATGGCCT AAACTATCTC 
AGCACCACTG TGAAAGAGAT GTCAAAGAAA GCACCACTGT CTGATATTAG TCGGAAATAT 
CAATCAGAAT TTGAAGAGAT TGAGGGACGT TGGAAGAAGC TGTCTTCCCA GCTGGTTGAA 
CATTGTCAAA AGTTGGAGGA GCAAATGGCT AAACTTCGAA AAATTCAGAA TCACATAAAA 
ACTCTGAAGA AATGGATCAC TGAAGTCGAT GTTTTCCTGA AGGAGGAATG GCCTGCCCTT 
GGGGATTCAG AAATTCTGAA AAGACAGCTG AAACAGTGCA GGCTTTTAGT CAATGACATT 
CAGACCATCC AGCCTAGTCT CAACAGTGTC AATGAAGGGG CTCAGAAGAT GAAGAATGAA 
GCAGAACCAG AGTTTGCTGG CAGACTTGAG ACAGAGCTCC GAGAACTTAA CACCCAGTGG 
GATTACATGT GCCGCCAGGT CTATGCCAGG AAGGAAGCCT TAAAAGGAGG TTTGGATAAA 
ACTGTAAGTC TTCAGAAAGA TCTGTCAGAG ATGCATGAGT GGATGACACA AGCTGAAGAA 
GAATACCTAG AGAGAGATTT CGAATACAAG ACCCCTGATG AATTACAGAC AGCAGTTGAA 
GAGATGAAGA GAGCTAAAGA AGAGGCCCAG CAAAAAGAAG CAAAAGTGAA ACTCCTAACC 
GAGTCCGTCA ATAGTGTCAT AGCTCAGGCT CCACCTGCAG CACAAGAGGC CTTAAAAAAG 
GAACTTGACA CTCTCACCAC CAACTACCAG TGGCTCTGCA CCAGGCTCAA TGGCAAATGC 
AAGACCTTGG AAGAAGTTTG GGCGTGCTGG CATGAGTTAT TGTCCTACTT GGAGAAGGCA 
AACAAGTGGC TAAGTGAAGT AGAAGTCAAG CTTAAAACCA CTGAAAATAT TTCTGGGGGA 
GCTGAGGAAA TCGCCGAGGT GCTTGATTCG CTTGAAAATT TGATGCAACA TTCAGAGGAT 
AACCCGAATC AGATTCGCAT ATTGGCACAG ACCTTGACAG ATGGTGGAGT CATGGATGAA 
CTGATCAATG AGGAGCTTGA GACATTTAAT TCTCGTTGGA GAGAACTCCA TGAAGAGGCT 
GTGAGGAGGC AAAAGTTGCT TGAGCAGAGT ATCCAGTCGG CCCAGGAGAT AGAAAAATCC 
TTGCACTTAA TTCAGGAGTC CCTCTCTTCC ATTGACAAGC AGTTGGCAGC TTATATTGCT 
GACAAAGTGG ATGCAGCTCA GATGCCTCAG GAAGCCCAGA AAATCCAATC AGATTTGACA 
AGTCATGAGA TCAGTTTAGA AGAAATGAAG AAACATAACC AGGGAAAGGA GACTGCCCAA 
AGGGTACTAT CCCAAATTGA TGTGGCACAG AAAAAATTGC AGGATGTTTC CATGAAGTTT 
CGATTATTCC AGAAGCCAGC CAATTTTGAG CAGCGCCTAC AAGAAAGTAA AATGATTTTA 
GATGAAGTGA AGATGCATTT ACCTGCGTTG GAAACAAAGA GTGTGGAACA GGAAGTAGTA 
CAGTCACAGT TAAATCATTG TGTGAACTTG TATAAAAGTC TGAGTGAAGT GAAGTCTGAA 
GTGGAAATGG TAATAAAAAC TGGACGTCAG ATTGTACAGA AGAAGCAGAC GGAAAACCCG 
AAAGAGCTTG ATGAAAGAGT TACAGCTTTG AAATTGCATT ATAATGAGCT GGGAGCAAAG 
GTGACAGAAA GAAAGCAACA GTTGGAAAAA TGCTTGAAAT TGTCCCGTAA GATGCGAAAG 
GAAATGAATG CCCTGACAGA ATGGCTGGCA GCTACAGATA TGGAACTGAC AAAGAGATCG 
GCAGTTGAAG GAATGCCTAG TAATTTGGAT TCTGAAGTTG CCTGGGGAAA GGCTACTCAG 
AAAGAGATTG AGAAACAGAA GGTTCACCTA AAGAGTGTCA CAGAGGTAGG AGAGGCCTTG 
AAAACGGTTT TGGGCAAGAA GGAAATGTTG GTGGAAGATA AACTGAGTCT TCTGAATAGT 
AACTGGATAG CCGTCACTTC CCGAGCAGAA GAGTGGTTAA ACCTTTTATT GGAATACCAG 
AAACACATGG AAACTTTTGA CCAGAATGTG GATTACATCA CAAACTGGAT CATTCAGGCT 
GATGCACTTT TGGATGAATC TGAGAAAAAG AAACCTCAGC AAAAAGAAGA CATACTTAAG 
CGTTTAAAGG CTGAAATGAA TGACATACGT CCAAAGGTGG ATTCTACACG TGACCAAGCA 
GCAAACCTGA TGGCAAACCG CGGCGACCAC TGCAGGAAAG TAGTAGAGCC CAAAATCTCA 
GAGCTCAACC ATCGATTTGC AGCCATTTCT CACAGAATTA AGACTGGAAA GGCCTCCATT 
CCTTTGAAGG AATTGGAGCA GTTTAACTCA GATATACAAA AATTGCTTGA ACCACTGGAG 
GCTGAAATTC AGCAGGGGGT GAATCTGAAA GAGGAAGACT TCAATAAAGA TATGAGTGAA 
GACAATGAGG GTACTGTAAA AGAATTGTTG CAAAGAGGAG ACAACTTACA ACAAAGAATC 
ACAGATGAGA GAAAGCGAGA GGAAATAAAG ATAAAACAAC AGCTGTTACA GACAAAACAT 
AATGCTCTCA AGGATTTGAG GTCTCAAAGA AGAAAAAAGG CTCTAGAAAT TTCTCACCAG 
TGGTATCAGT ACAAGAGGCA GGCTGATGAT CTCCTGAAAT GCTTGGATGA CATTGAAAAA 
AAATTAGCCA GCCTACCTGA ACCCAGAGAT GAAAGGAAAA TAAAGGAAAT TGATCGTGAA 
TTGCAGAAGA AGAAAGAGGA GCTGAATGCA GTGCGTAGGC AAGCTGAGGG CTTGTCTGAG 
GATGGGGCCG CAATGGCAGT GGAGCCAACT CAGATCCAGC TCAGCAAGCG CTGGCGGGAA 
ATTGAGAGCA AATTTGCTCA GTTTCGAAGA CTCAACTTTG CACAAATTCA CACTGTCCAT 
GAAGAGTCAG TGGTGGCGAT GACTGAAGAC ATGCCTTTGG AAATTTCTTA TGTGCCTTCT 
ACTTACCTGA CTGAGATCAC TCATGTCTCA CAAGCCCTAT CAGAAGTGGA AGAGCTTCTT 
AATGCTCCCG ACCTTTGTGC TCAAGATTTT GAAGATCTCT TTAAACAAGA GGAATCCTTG 
AAGAACATAA AAGACAGCCT GCAACAAATC TCAGGTCGGA TTGACATCAT TCACAATAAA 
AAGACAGCAG CATTGCACAG TGCCACTCCT GCAGAAAGGG CAAAGCTCCA GGAAGCTCTC 
TCACGGCTTG ATTTCCAATG GGAAAGAGTT AACAATATGT ACAAGGACCG ACAAGGGAGA 
TTTGACAGAT CTGTGGAAAA ATGGCGGCGG TTTCATTATG ATATGAAGAT ACTTAATCAA 
TGGCTAACAG AAGCTGAACA GTTTCTCAAA AAGACACAAA TTCCTGAGAA TTGGGAACAT 
GCCAAATACA AATGGTATCT TAAGGAACTC CAGGATGGCA TTGGACAGCG GCAAAGTGTT 
GTCAGGGTAT TGAATGCAAC TGGGGAAGAA ATAATTCAAC AGTCCTCAAA AACAGATGCC 
AGTATTCTCC AAGAAAAACT GGGAAGCCTG AATCTGCGGT GGCAGGAGGT CTGCAAACAG 
CTGGCAGAAA GAAAAAAGAG GCTAGAGGAA CAGAAGAATA TCTTGTCAGA ATTTCAAAGA 
GATGTAAATG AATTTGTTTT ATGGTTGGAA GAAGCGGATA ACGTTGCTAA TATTCCACTT 
GAACCTGGAA ATGAGCAGCA GCTAAAAGAA AAACTTGAAC AAGTCAAGTT ACTGGCAGAA 
GAGTTGCCCC TGCGCCAGGG AATTCTAAAA CAATTAAATG AAACTGGAGG AACAGTGCTT 
GTAAGTGCTC CCCTAAGCCC AGAAGAGCAA GATAAACTTG AAAATAAGCT CAAGCAGACA 
AATCTTCAGT GGATAAAGGT TTCTAGAAAT CTGCCTGAGA AGCAAGAAGA AATTGAGGCA 
CACGTAAAAG ACCTTGGACA GCTGGAAGAG CAGTTAAATC ATCTGCTTCT ATGGCTGTCT 
CCTATTAGGA ATCAGTTGGA AATTTACAAT CAGCCAAATC AAACAGGACC ATTTGACATC 
AAGGAAATTG AAGTAGCAGT TCAAGCTAAA CAGCCGGATG TGGAAGGGAT TTTGTCTAAA 
GGGCAGCATT TGTACAAGGA AAAACCAGCC ACTCAGCCAG CGAAGAGAAA GCTGGAAGAT 
CTCAGCTCTG ATTGGAAGGT GGTAACTCAG TTGCTTCAAG AGCTGCGGGC AAAGCAACCT 
GGCCCAGCTC CTGGACTGAC CACTGTCAGA GCCCCTCCCA GTCAGACTGT TACTCTGGTG 
ACACAACCCG CGGTTACCAA GGAAACTGCC ATCTCCAAAC TAGAAATGCC ATCTTCATTG 
CTGTTGGAGG TACCTGCACT GGCAGATTTC AACCGAGCTT GGACAGAACT TACCGACTGG 
CTGTCTCTGC TTGATCGAGT TATAAAATCA CAGAGGGTGA TGGTGGGTGA TCTTGAAGAC 
ATTAACGAGA TGATCATCAA GCAGAAGGCA ACGCTGCAGG ATTTGGAACA GAGGCGCCCC 
CAGTTGGAAG AACTCATTAC CGCTGCCCAG AATTTGAAAA ACAAGACCAG CAATCAAGAG 
GCTAGAACAA TCATTACTGA TCGAATTGAA AGAATTCAGA GTCAGTGGGA TGAAGTACAG 
GAACATCTTC AGAACCGGAG GCTACAGTTG ACTGAAATGT TAAAGGATTC CACACAATGG 
CTGGAAGCTA AAGAGGAGGC TGAGCAGGTG TTGGGGCAGG CCAGAGCCAA GCTTGAGTCA 
TGGAAGGAGG CTCCCTACAC AGTAGATGCA ATCCAAAAGA AAATCACAGA AACCAAGCAG 
TTGGCCAAAG ACCTCCGCCA GTGGCAGATA AATGTAGATG TTGCAAATGA TTTGGCACTG 
AAACTTCTCC GAGATTATTC TGCAGATGAT ACCAGAAAAG TACACATGAT AACAGAGAAC 
ATCAATGCCT CTTGGGCAAG CATCCATAAA AGATTGAGTG AGCGAGAGGC TGCTCTGGAA 
GAAACCCACA GATTACTGCA ACAGTTCCCC TTGGACCTGG AGAAGTTCCT TGCCTGGCTT 
ACAGAAGCCG AAACAACTGC CAACGTCCTG CAGGATGCCA CCCATAAGGA AAGGCTTCTA 
GAAGATTCCA AGGGAGTAAG AGAGCTGATG AAACAATGGC AAGACCTCCA AGGAGAAATC 
GAAGCTCACA CAGATATCTA TCACAACCTG GACGAAAATG GCCAAAAAGT CCTGAGATCC 
CTGGAAGGTT CTGACGATGC AGCCTTGTTG CAAAGACGTT TGGATAACAT GAACTTCAAG 
TGGAGCGAAC TTCGGAAAAA GTCTCTCAAC ATTAGGTCTC ACTTGGAAGC CAGTTCTGAC 
CAGTGGAAGC GTCTGCACCT TTCTCTTCAG GAACTTCTGG TATGGCTCCA GCTGAAAGAT 
GATGAGTTAA GCCGGCAGGC ACCCATTGGA GGAGACTTTC CAGCGGTGCA GAAGCAGAAT 
GATGTACACA GGGCCTTCAA GAGGGAATTG AAAACGAAAG AACCTGTAAT CATGAGTACT 
CTTGAGACTG TACGAATATT TCTGACAGAG CAGCCTTTAG AAGGACTAGA GAAACTCTAC 
CAGGAGCCCA GAGAGCTGCC TCCTGAAGAG AGAGCCCAGA ATGTCACACG GCTCCTACGA 
AAGCAAGCTG AGGAGGTCAA CACTCAGTGG GAAAAACTGA ACGTGCACTC TGCAGACTGG 
CAGAGAAAAA TAGACGAGGC CCTCGAAAGA CTCCAGGAGC TTCAGGAAGC AACAGATGAG 
CTGGATCTCA AACTACGTCA GGCAGAGGTG ATCAAAGGAT CCTGGCAGCC TGTGGGTGAC 
CTCCTCATTG ACTCTCTCCA AGATCACCTC GAAAAAGTCA AGGCGCTTCG AGGAGAAATT 
ACACCTCTGA AAGAGAATGT CAGCTACGTC AATGACCTTG CTCGCCAACT CACTACGTTG 
GGCATTCAGC TGTCACCATA TAACCTCAAC ACTCTGGAAG ACCTGAACAC CAGATGGAAG 
CTTCTGCAGG TGGCCATTGA GGACCGCATC AGGCAGCTGC ATGAAGCGCA CAGGGACTTT 
GGACCAGCCT CCCAGCACTT CCTTTCCACT TCTGTCCAGG GTCCCTGGGA GAGAGCCATC 
TCACCAAACA AAGTGCCCTA CTATATCAAC CACGAGACCC AAACAACTTG CTGGGACCAT 
CCCAAAATGA CAGAGCTCTA CCAGTCTTTA GCTGACCTGA ATAATGTCAG GTTCTCAGCT 
TACAGGACTG CCATGAAACT CCGAAGACTG CAGAAGGCCC TTTGCTTGGA TCTCTTGAGC 
CTATCGGCTG CATGCGATGC CTTGGACCAG CACAACCTCA AGCAAAATGA CCAGCCCATG 
GATATCCTGC AGGTCATTAA CTGTCTGACC ACTATTTATG ATCGCCTAGA GCAAGAGCAC 
AACAATCTGG TCAACGTCCC TCTCTGCGTG GATATGTGTC TCAATTGGCT GCTGAATGTT 
TATGACACGG GACGAACGGG GAGGATCCGG GTCCTGTCTT TTAAAACTGG CATCATTTCT 
CTGTGTAAAG CCCATTTGGA AGACAAGTAC AGATACCTCT TCAAGCAAGT GGCAAGTTCG 
ACAGGATTTT GTGACCAGCG CAGGCTGGGC CTCCTCCTGC ATGACTCTAT CCAGATCCCA 
AGACAGTTGG GTGAAGTCGC ATCGTTCGGG GGCAGTAACA TTGAGCCGAG TGTCAGGAGC 
TGCTTCCAGT TTGCTAATAA TAAGCCTGAG ATCGAAGCGG CCCTCTTCCT AGACTGGATG 
CGCCTGGAGC CCCAGTCCAT GGTGTGGCTG CCTGTCCTGC ACCGAGTGGC TGCCGCGGAA 
ACTGCCAAGC ACCAGGCCAA GTGCAACATC TGCAAGGAGT GTCCCATCAT CGGATTCAGG 
TACAGGAGTC TAAAGCACTT TAATTATGAC ATCTGCCAAA GTTGCTTTTT TTCTGGTCGA 
GTTGCAAAAG GCCATAAAAT GCACTATCCC ATGGTGGAAT ACTGCACTCC GACTACATCG 
GGAGAAGATG TCCGTGACTT TGCCAAGGTA CTAAAAAACA AATTTCGAAC CAAAAGGTAT 
TTTGCGAAGC ATCCCCGAAT GGGCTACCTG CCAGTGCAGA CTGTCTTAGA GGGGGACAAC 
ATGGAAACTC CTGTCACTCT GATCAACTTC TGGCCGGTAG ATTCTGCGCC TGCCTCGTCC 
CCTCAGCTTT CACACGATGA TACTCATTCA CGCATTGAGC ATTATGCTAG CAGGCTAAAA 
AAAATGGAAA ACAGCAATGG ATCTTATCTA AATGATAGCA TCTCTCCTAA TGAGAGCATA 
GATGATGAAC ATTTGTTAAT CCAGCATTAC TGGCGAAGTT TGAACCAGGA ATCCCCCCTG 
AGCCAGCCTC GTAGTCCTGC CCAGATCTTG ATTTCCTTAG AGAGTGAGGA AAGAGGGGAG 
CTAGAGAGAA TCCTAGCAGA TCTTGAGGGG AGAAACAGAA ATCTGCAAGC AGAGTATGAT 
CGTCTAAAGC AGCAGCATGA ACACAAAGGC CTGTCCCCAC TGCCATCCCC TCCTGAAATG 
ATGCCTACTT CTCCCCAAAG TCCCCGGGAT GCTGAGCTCA TCGCTGAGGC CAAGCTGCTG 
CGTCAACACA AAGGCCGCCT GGAAGCCAGG ATGCAAATCC TTGAAGACCA TAACAAACAA 
CTGGAATCCC AGTTACACAG GCTCAGGCAG CTGCTGGAGC AACCCCAGGC AGAGGCCAAG 
GTGAATGGTA CAACGGTGTC TTCTCCTTCT ACCTCTCTTC AGAGGTCAGA TAGCAGTCAG 
CCTATGCTGC TCCGGGTGGT CGGCAGTCAG ACTTCAGAAT CCATGGGCGA GGAAGACCTG 
CTCAGCCCTC CCCAGGACAC AAGCACAGGG TTAGAGGAAG TGATGGAGCA GCTCAACCAC 
TCCTTCCCTA GTTCCAGAGG AAGAAATACC CCTGGGAAGC CAATGAGAGA GGACACAATG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003343.1
CDS	290..11332
Translation	MLWWEEVEDCYEREDVQKKTFTKWVNAQFSKFGKQHIENLFSDLQDGRRLLDLLEGLTGQKLPKEKGSTRVHALNNVNKALRVLQKNNVDLVNIGSTDIVDGNHKLTLGLIWNIILHWQVKNVMKNIMAGLQQTNSEKILLSWVRQSTRNYPQVNVINFTTSWSDGLALNALIHSHRPDLFDWNSVVCQQSATQRLEHAFNIAKYQLGIEKLLDPEDVATTYPDKKSILMYITSLFQVLPQQVSIEAIQEVEMLPRPSQVTREEHFQIHHQMHYSQQITVSLAQGYERAPSFPKPRFKSYAYTQAAYVTTSDPTRSPLPSQHLETPEDKSFGRSLTETEANLDSYQTALEEVLSWLLSAEDALQAQGEISNDVEEVKEQFHTHEGYMMDLTSHQGRVGNVLQLGSQLIGTGKLSEDEETEVQEQMNLLNSRWECLRVASMEKQSNLHKVLMDLQNQQLKELNDWLTKTEERTRKMEKEPLGPDIEDLKRQVQQHKVLQEDLEQEQVRVNSLTHMVVVVDESSGDHATAALEEQLKVLGDRWANICRWTEDRWVLLQDILLKWQRFTEEQCLFSAWLSEKEDAVNKIHTTGFKDQSEVLSNLQKLAVLKTDLEKKKQTMDKLCSLNQDLLSALKNTVVAHKMEAWLDNFAQRWDNLVQKLEKSSAQISQAVTTTQPSLTQTTVMETVTMVTTREHILVKHAQEELPPPPPQKKRQIIVDSEIRKRLDVDITELHSWITRSEAVLQSPEFAIYRKEGNFSDLKEKVNAIEREKAEKFRKLQDASRSAQALVEQMVNEGVNADSIKQASEQLNSRWIEFCQLLSERLNWLEYQNNIITFYNQLQQLEQMTTTAENWLKTQPTTTSEPTAIKSQLKICKDEINRLSALQPQIERLKIQSIALKEKGQGPMFLDADFVAFTNHFNQVFADVQAREKELQTIFDSLPPMRYQETMSTILTWIQQSETKLSIPQVTVTEYDIMEQRLGELQALQSSLQEQQNGLNYLSTTVKEMSKKAPLSDISRKYQSEFEEIEGRWKKLSSQLVEHCQKLEEQMAKLRKIQNHIKTLKKWITEVDVFLKEEWPALGDSEILKRQLKQCRLLVNDIQTIQPSLNSVNEGAQKMKNEAEPEFAGRLETELRELNTQWDYMCRQVYARKEALKGGLDKTVSLQKDLSEMHEWMTQAEEEYLERDFEYKTPDELQTAVEEMKRAKEEAQQKEAKVKLLTESVNSVIAQAPPAAQEALKKELDTLTTNYQWLCTRLNGKCKTLEEVWACWHELLSYLEKANKWLSEVEVKLKTTENISGGAEEIAEVLDSLENLMQHSEDNPNQIRILAQTLTDGGVMDELINEELETFNSRWRELHEEAVRRQKLLEQSIQSAQEIEKSLHLIQESLSSIDKQLAAYIADKVDAAQMPQEAQKIQSDLTSHEISLEEMKKHNQGKETAQRVLSQIDVAQKKLQDVSMKFRLFQKPANFEQRLQESKMILDEVKMHLPALETKSVEQEVVQSQLNHCVNLYKSLSEVKSEVEMVIKTGRQIVQKKQTENPKELDERVTALKLHYNELGAKVTERKQQLEKCLKLSRKMRKEMNALTEWLAATDMELTKRSAVEGMPSNLDSEVAWGKATQKEIEKQKVHLKSVTEVGEALKTVLGKKEMLVEDKLSLLNSNWIAVTSRAEEWLNLLLEYQKHMETFDQNVDYITNWIIQADALLDESEKKKPQQKEDILKRLKAEMNDIRPKVDSTRDQAANLMANRGDHCRKVVEPKISELNHRFAAISHRIKTGKASIPLKELEQFNSDIQKLLEPLEAEIQQGVNLKEEDFNKDMSEDNEGTVKELLQRGDNLQQRITDERKREEIKIKQQLLQTKHNALKDLRSQRRKKALEISHQWYQYKRQADDLLKCLDDIEKKLASLPEPRDERKIKEIDRELQKKKEELNAVRRQAEGLSEDGAAMAVEPTQIQLSKRWREIESKFAQFRRLNFAQIHTVHEESVVAMTEDMPLEISYVPSTYLTEITHVSQALSEVEELLNAPDLCAQDFEDLFKQEESLKNIKDSLQQISGRIDIIHNKKTAALHSATPAERAKLQEALSRLDFQWERVNNMYKDRQGRFDRSVEKWRRFHYDMKILNQWLTEAEQFLKKTQIPENWEHAKYKWYLKELQDGIGQRQSVVRVLNATGEEIIQQSSKTDASILQEKLGSLNLRWQEVCKQLAERKKRLEEQKNILSEFQRDVNEFVLWLEEADNVANIPLEPGNEQQLKEKLEQVKLLAEELPLRQGILKQLNETGGTVLVSAPLSPEEQDKLENKLKQTNLQWIKVSRNLPEKQEEIEAHVKDLGQLEEQLNHLLLWLSPIRNQLEIYNQPNQTGPFDIKEIEVAVQAKQPDVEGILSKGQHLYKEKPATQPAKRKLEDLSSDWKVVTQLLQELRAKQPGPAPGLTTVRAPPSQTVTLVTQPAVTKETAISKLEMPSSLLLEVPALADFNRAWTELTDWLSLLDRVIKSQRVMVGDLEDINEMIIKQKATLQDLEQRRPQLEELITAAQNLKNKTSNQEARTIITDRIERIQSQWDEVQEHLQNRRLQLTEMLKDSTQWLEAKEEAEQVLGQARAKLESWKEAPYTVDAIQKKITETKQLAKDLRQWQINVDVANDLALKLLRDYSADDTRKVHMITENINASWASIHKRLSEREAALEETHRLLQQFPLDLEKFLAWLTEAETTANVLQDATHKERLLEDSKGVRELMKQWQDLQGEIEAHTDIYHNLDENGQKVLRSLEGSDDAALLQRRLDNMNFKWSELRKKSLNIRSHLEASSDQWKRLHLSLQELLVWLQLKDDELSRQAPIGGDFPAVQKQNDVHRAFKRELKTKEPVIMSTLETVRIFLTEQPLEGLEKLYQEPRELPPEERAQNVTRLLRKQAEEVNTQWEKLNVHSADWQRKIDEALERLQELQEATDELDLKLRQAEVIKGSWQPVGDLLIDSLQDHLEKVKALRGEITPLKENVSYVNDLARQLTTLGIQLSPYNLNTLEDLNTRWKLLQVAIEDRIRQLHEAHRDFGPASQHFLSTSVQGPWERAISPNKVPYYINHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQVINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPVTLINFWPVDSAPASSPQLSHDDTHSRIEHYASRLKKMENSNGSYLNDSISPNESIDDEHLLIQHYWRSLNQESPLSQPRSPAQILISLESEERGELERILADLEGRNRNLQAEYDRLKQQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDTSTGLEEVMEQLNHSFPSSRGRNTPGKPMREDTM

Target ORF information:

RefSeq Version	NM_001003343.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris dystrophin (DMD), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003343.1

ORF Insert Sequence:

ATGCTTTGGT GGGAAGAAGT AGAGGACTGT TATGAAAGAG AAGATGTTCA AAAGAAAACA 
TTCACAAAAT GGGTAAATGC ACAGTTTTCT AAGTTTGGGA AGCAGCACAT AGAGAACCTC 
TTCAGTGACC TACAGGATGG GAGACGCCTC CTAGACCTTT TGGAAGGCCT GACAGGGCAA 
AAACTGCCAA AAGAAAAAGG ATCCACAAGA GTTCATGCCC TGAACAATGT CAACAAGGCA 
CTGCGCGTCT TGCAGAAAAA TAATGTTGAT TTAGTGAACA TTGGAAGTAC TGACATAGTA 
GATGGAAATC ACAAACTGAC TCTTGGTTTG ATTTGGAATA TAATCCTCCA CTGGCAGGTC 
AAAAATGTAA TGAAAAATAT CATGGCTGGA TTGCAACAAA CCAACAGTGA AAAGATTCTC 
CTGAGCTGGG TCCGACAATC AACTCGTAAT TATCCACAGG TTAATGTCAT TAACTTCACC 
ACCAGCTGGT CTGATGGCCT GGCTTTGAAC GCTCTCATCC ACAGTCATAG GCCAGACCTG 
TTTGATTGGA ATAGTGTGGT TTGCCAGCAG TCAGCCACAC AACGCCTGGA ACATGCATTC 
AACATTGCCA AATATCAATT AGGCATAGAG AAACTGCTTG ATCCTGAAGA TGTTGCCACC 
ACTTATCCAG ATAAGAAGTC CATCTTAATG TATATCACAT CACTCTTCCA AGTTTTGCCT 
CAACAAGTGA GCATTGAAGC CATCCAGGAA GTGGAAATGT TGCCAAGGCC ATCTCAAGTT 
ACTAGAGAAG AACATTTTCA GATACATCAT CAAATGCACT ATTCTCAACA GATCACAGTC 
AGTCTAGCAC AGGGATATGA ACGAGCCCCT TCCTTTCCTA AGCCTCGGTT CAAGAGCTAT 
GCCTACACAC AGGCTGCTTA TGTCACCACT TCTGACCCCA CACGGAGCCC ACTTCCTTCA 
CAGCATTTGG AAACTCCTGA AGACAAGTCA TTTGGCCGGT CATTGACAGA GACCGAAGCA 
AACCTGGACA GTTATCAAAC AGCTTTGGAA GAAGTACTCT CGTGGCTTCT TTCAGCTGAG 
GATGCACTGC AAGCCCAAGG AGAGATTTCT AATGATGTCG AAGAAGTGAA AGAACAATTT 
CATACTCATG AGGGATATAT GATGGACTTG ACATCCCATC AGGGACGGGT CGGTAATGTT 
CTCCAACTGG GAAGTCAACT GATTGGAACA GGGAAATTAT CAGAAGATGA AGAAACCGAA 
GTGCAGGAAC AAATGAATCT CCTCAATTCA AGATGGGAAT GCCTCAGGGT AGCTAGCATG 
GAAAAACAAA GCAATTTACA TAAAGTTCTA ATGGATCTCC AGAATCAGCA ACTGAAAGAG 
TTAAATGACT GGCTAACCAA AACAGAAGAG AGAACAAGGA AAATGGAGAA GGAGCCCCTT 
GGACCTGATA TTGAAGACCT AAAACGCCAA GTACAACAAC ATAAGGTGCT TCAAGAAGAC 
TTAGAACAGG AACAAGTCAG GGTCAATTCC CTCACTCATA TGGTGGTGGT AGTCGATGAA 
TCTAGTGGAG ACCATGCAAC TGCTGCTTTG GAAGAACAAC TTAAGGTACT GGGAGATCGA 
TGGGCAAACA TCTGTAGGTG GACAGAAGAT CGCTGGGTTC TTTTACAAGA CATCCTCCTA 
AAATGGCAGC GTTTTACTGA AGAACAGTGC CTTTTTAGTG CATGGCTTTC GGAGAAGGAA 
GATGCAGTGA ACAAGATTCA CACAACTGGC TTTAAGGATC AAAGTGAAGT GTTATCAAAT 
CTTCAGAAAC TGGCTGTCTT AAAAACAGAT CTGGAAAAGA AGAAGCAAAC CATGGACAAA 
CTCTGCTCAC TCAACCAAGA CCTTCTTTCA GCGCTGAAAA ACACAGTGGT AGCCCACAAG 
ATGGAAGCAT GGCTGGACAA CTTTGCCCAG CGCTGGGATA ATTTAGTCCA GAAACTTGAA 
AAAAGTTCAG CACAGATTTC ACAGGCTGTC ACCACCACTC AGCCATCACT AACACAGACA 
ACTGTAATGG AAACAGTAAC TATGGTGACC ACGAGGGAAC ACATCTTGGT AAAGCATGCC 
CAAGAGGAAC TGCCACCACC ACCCCCTCAG AAGAAGAGGC AGATTATCGT GGATTCTGAA 
ATTAGGAAAA GGTTGGATGT CGATATAACT GAACTTCACA GTTGGATTAC TCGTTCAGAA 
GCTGTGTTGC AGAGTCCTGA ATTTGCAATC TATCGGAAGG AAGGCAACTT CTCAGACCTT 
AAAGAAAAAG TCAATGCCAT AGAGCGAGAA AAAGCCGAGA AGTTCAGAAA ACTGCAAGAT 
GCCAGCAGAT CAGCTCAGGC CCTGGTGGAA CAGATGGTGA ATGAGGGTGT TAATGCTGAC 
AGCATCAAAC AAGCCTCCGA ACAACTGAAC AGCCGGTGGA TAGAGTTCTG CCAATTGCTA 
AGCGAGAGAC TTAACTGGCT GGAGTATCAG AACAACATCA TCACTTTCTA TAATCAGCTA 
CAACAATTGG AGCAGATGAC AACTACTGCT GAAAACTGGT TGAAAACCCA GCCTACCACC 
ACATCAGAGC CAACAGCAAT TAAAAGCCAG TTAAAAATTT GTAAGGATGA AATCAACCGA 
CTGTCAGCTC TTCAGCCTCA AATCGAGCGA TTAAAAATTC AAAGCATAGC CCTGAAAGAG 
AAAGGACAAG GGCCAATGTT CCTGGATGCA GACTTTGTGG CCTTTACAAA TCATTTTAAC 
CAAGTCTTTG CTGATGTGCA GGCAAGAGAA AAAGAGCTAC AAACAATTTT TGACAGTTTG 
CCACCCATGC GCTATCAGGA GACTATGAGT ACCATCCTGA CATGGATCCA GCAGTCAGAA 
ACCAAACTCT CTATACCTCA GGTTACTGTC ACTGAATATG ACATCATGGA ACAGAGACTC 
GGAGAGCTAC AGGCTTTACA AAGCTCTCTG CAAGAGCAAC AAAATGGCCT AAACTATCTC 
AGCACCACTG TGAAAGAGAT GTCAAAGAAA GCACCACTGT CTGATATTAG TCGGAAATAT 
CAATCAGAAT TTGAAGAGAT TGAGGGACGT TGGAAGAAGC TGTCTTCCCA GCTGGTTGAA 
CATTGTCAAA AGTTGGAGGA GCAAATGGCT AAACTTCGAA AAATTCAGAA TCACATAAAA 
ACTCTGAAGA AATGGATCAC TGAAGTCGAT GTTTTCCTGA AGGAGGAATG GCCTGCCCTT 
GGGGATTCAG AAATTCTGAA AAGACAGCTG AAACAGTGCA GGCTTTTAGT CAATGACATT 
CAGACCATCC AGCCTAGTCT CAACAGTGTC AATGAAGGGG CTCAGAAGAT GAAGAATGAA 
GCAGAACCAG AGTTTGCTGG CAGACTTGAG ACAGAGCTCC GAGAACTTAA CACCCAGTGG 
GATTACATGT GCCGCCAGGT CTATGCCAGG AAGGAAGCCT TAAAAGGAGG TTTGGATAAA 
ACTGTAAGTC TTCAGAAAGA TCTGTCAGAG ATGCATGAGT GGATGACACA AGCTGAAGAA 
GAATACCTAG AGAGAGATTT CGAATACAAG ACCCCTGATG AATTACAGAC AGCAGTTGAA 
GAGATGAAGA GAGCTAAAGA AGAGGCCCAG CAAAAAGAAG CAAAAGTGAA ACTCCTAACC 
GAGTCCGTCA ATAGTGTCAT AGCTCAGGCT CCACCTGCAG CACAAGAGGC CTTAAAAAAG 
GAACTTGACA CTCTCACCAC CAACTACCAG TGGCTCTGCA CCAGGCTCAA TGGCAAATGC 
AAGACCTTGG AAGAAGTTTG GGCGTGCTGG CATGAGTTAT TGTCCTACTT GGAGAAGGCA 
AACAAGTGGC TAAGTGAAGT AGAAGTCAAG CTTAAAACCA CTGAAAATAT TTCTGGGGGA 
GCTGAGGAAA TCGCCGAGGT GCTTGATTCG CTTGAAAATT TGATGCAACA TTCAGAGGAT 
AACCCGAATC AGATTCGCAT ATTGGCACAG ACCTTGACAG ATGGTGGAGT CATGGATGAA 
CTGATCAATG AGGAGCTTGA GACATTTAAT TCTCGTTGGA GAGAACTCCA TGAAGAGGCT 
GTGAGGAGGC AAAAGTTGCT TGAGCAGAGT ATCCAGTCGG CCCAGGAGAT AGAAAAATCC 
TTGCACTTAA TTCAGGAGTC CCTCTCTTCC ATTGACAAGC AGTTGGCAGC TTATATTGCT 
GACAAAGTGG ATGCAGCTCA GATGCCTCAG GAAGCCCAGA AAATCCAATC AGATTTGACA 
AGTCATGAGA TCAGTTTAGA AGAAATGAAG AAACATAACC AGGGAAAGGA GACTGCCCAA 
AGGGTACTAT CCCAAATTGA TGTGGCACAG AAAAAATTGC AGGATGTTTC CATGAAGTTT 
CGATTATTCC AGAAGCCAGC CAATTTTGAG CAGCGCCTAC AAGAAAGTAA AATGATTTTA 
GATGAAGTGA AGATGCATTT ACCTGCGTTG GAAACAAAGA GTGTGGAACA GGAAGTAGTA 
CAGTCACAGT TAAATCATTG TGTGAACTTG TATAAAAGTC TGAGTGAAGT GAAGTCTGAA 
GTGGAAATGG TAATAAAAAC TGGACGTCAG ATTGTACAGA AGAAGCAGAC GGAAAACCCG 
AAAGAGCTTG ATGAAAGAGT TACAGCTTTG AAATTGCATT ATAATGAGCT GGGAGCAAAG 
GTGACAGAAA GAAAGCAACA GTTGGAAAAA TGCTTGAAAT TGTCCCGTAA GATGCGAAAG 
GAAATGAATG CCCTGACAGA ATGGCTGGCA GCTACAGATA TGGAACTGAC AAAGAGATCG 
GCAGTTGAAG GAATGCCTAG TAATTTGGAT TCTGAAGTTG CCTGGGGAAA GGCTACTCAG 
AAAGAGATTG AGAAACAGAA GGTTCACCTA AAGAGTGTCA CAGAGGTAGG AGAGGCCTTG 
AAAACGGTTT TGGGCAAGAA GGAAATGTTG GTGGAAGATA AACTGAGTCT TCTGAATAGT 
AACTGGATAG CCGTCACTTC CCGAGCAGAA GAGTGGTTAA ACCTTTTATT GGAATACCAG 
AAACACATGG AAACTTTTGA CCAGAATGTG GATTACATCA CAAACTGGAT CATTCAGGCT 
GATGCACTTT TGGATGAATC TGAGAAAAAG AAACCTCAGC AAAAAGAAGA CATACTTAAG 
CGTTTAAAGG CTGAAATGAA TGACATACGT CCAAAGGTGG ATTCTACACG TGACCAAGCA 
GCAAACCTGA TGGCAAACCG CGGCGACCAC TGCAGGAAAG TAGTAGAGCC CAAAATCTCA 
GAGCTCAACC ATCGATTTGC AGCCATTTCT CACAGAATTA AGACTGGAAA GGCCTCCATT 
CCTTTGAAGG AATTGGAGCA GTTTAACTCA GATATACAAA AATTGCTTGA ACCACTGGAG 
GCTGAAATTC AGCAGGGGGT GAATCTGAAA GAGGAAGACT TCAATAAAGA TATGAGTGAA 
GACAATGAGG GTACTGTAAA AGAATTGTTG CAAAGAGGAG ACAACTTACA ACAAAGAATC 
ACAGATGAGA GAAAGCGAGA GGAAATAAAG ATAAAACAAC AGCTGTTACA GACAAAACAT 
AATGCTCTCA AGGATTTGAG GTCTCAAAGA AGAAAAAAGG CTCTAGAAAT TTCTCACCAG 
TGGTATCAGT ACAAGAGGCA GGCTGATGAT CTCCTGAAAT GCTTGGATGA CATTGAAAAA 
AAATTAGCCA GCCTACCTGA ACCCAGAGAT GAAAGGAAAA TAAAGGAAAT TGATCGTGAA 
TTGCAGAAGA AGAAAGAGGA GCTGAATGCA GTGCGTAGGC AAGCTGAGGG CTTGTCTGAG 
GATGGGGCCG CAATGGCAGT GGAGCCAACT CAGATCCAGC TCAGCAAGCG CTGGCGGGAA 
ATTGAGAGCA AATTTGCTCA GTTTCGAAGA CTCAACTTTG CACAAATTCA CACTGTCCAT 
GAAGAGTCAG TGGTGGCGAT GACTGAAGAC ATGCCTTTGG AAATTTCTTA TGTGCCTTCT 
ACTTACCTGA CTGAGATCAC TCATGTCTCA CAAGCCCTAT CAGAAGTGGA AGAGCTTCTT 
AATGCTCCCG ACCTTTGTGC TCAAGATTTT GAAGATCTCT TTAAACAAGA GGAATCCTTG 
AAGAACATAA AAGACAGCCT GCAACAAATC TCAGGTCGGA TTGACATCAT TCACAATAAA 
AAGACAGCAG CATTGCACAG TGCCACTCCT GCAGAAAGGG CAAAGCTCCA GGAAGCTCTC 
TCACGGCTTG ATTTCCAATG GGAAAGAGTT AACAATATGT ACAAGGACCG ACAAGGGAGA 
TTTGACAGAT CTGTGGAAAA ATGGCGGCGG TTTCATTATG ATATGAAGAT ACTTAATCAA 
TGGCTAACAG AAGCTGAACA GTTTCTCAAA AAGACACAAA TTCCTGAGAA TTGGGAACAT 
GCCAAATACA AATGGTATCT TAAGGAACTC CAGGATGGCA TTGGACAGCG GCAAAGTGTT 
GTCAGGGTAT TGAATGCAAC TGGGGAAGAA ATAATTCAAC AGTCCTCAAA AACAGATGCC 
AGTATTCTCC AAGAAAAACT GGGAAGCCTG AATCTGCGGT GGCAGGAGGT CTGCAAACAG 
CTGGCAGAAA GAAAAAAGAG GCTAGAGGAA CAGAAGAATA TCTTGTCAGA ATTTCAAAGA 
GATGTAAATG AATTTGTTTT ATGGTTGGAA GAAGCGGATA ACGTTGCTAA TATTCCACTT 
GAACCTGGAA ATGAGCAGCA GCTAAAAGAA AAACTTGAAC AAGTCAAGTT ACTGGCAGAA 
GAGTTGCCCC TGCGCCAGGG AATTCTAAAA CAATTAAATG AAACTGGAGG AACAGTGCTT 
GTAAGTGCTC CCCTAAGCCC AGAAGAGCAA GATAAACTTG AAAATAAGCT CAAGCAGACA 
AATCTTCAGT GGATAAAGGT TTCTAGAAAT CTGCCTGAGA AGCAAGAAGA AATTGAGGCA 
CACGTAAAAG ACCTTGGACA GCTGGAAGAG CAGTTAAATC ATCTGCTTCT ATGGCTGTCT 
CCTATTAGGA ATCAGTTGGA AATTTACAAT CAGCCAAATC AAACAGGACC ATTTGACATC 
AAGGAAATTG AAGTAGCAGT TCAAGCTAAA CAGCCGGATG TGGAAGGGAT TTTGTCTAAA 
GGGCAGCATT TGTACAAGGA AAAACCAGCC ACTCAGCCAG CGAAGAGAAA GCTGGAAGAT 
CTCAGCTCTG ATTGGAAGGT GGTAACTCAG TTGCTTCAAG AGCTGCGGGC AAAGCAACCT 
GGCCCAGCTC CTGGACTGAC CACTGTCAGA GCCCCTCCCA GTCAGACTGT TACTCTGGTG 
ACACAACCCG CGGTTACCAA GGAAACTGCC ATCTCCAAAC TAGAAATGCC ATCTTCATTG 
CTGTTGGAGG TACCTGCACT GGCAGATTTC AACCGAGCTT GGACAGAACT TACCGACTGG 
CTGTCTCTGC TTGATCGAGT TATAAAATCA CAGAGGGTGA TGGTGGGTGA TCTTGAAGAC 
ATTAACGAGA TGATCATCAA GCAGAAGGCA ACGCTGCAGG ATTTGGAACA GAGGCGCCCC 
CAGTTGGAAG AACTCATTAC CGCTGCCCAG AATTTGAAAA ACAAGACCAG CAATCAAGAG 
GCTAGAACAA TCATTACTGA TCGAATTGAA AGAATTCAGA GTCAGTGGGA TGAAGTACAG 
GAACATCTTC AGAACCGGAG GCTACAGTTG ACTGAAATGT TAAAGGATTC CACACAATGG 
CTGGAAGCTA AAGAGGAGGC TGAGCAGGTG TTGGGGCAGG CCAGAGCCAA GCTTGAGTCA 
TGGAAGGAGG CTCCCTACAC AGTAGATGCA ATCCAAAAGA AAATCACAGA AACCAAGCAG 
TTGGCCAAAG ACCTCCGCCA GTGGCAGATA AATGTAGATG TTGCAAATGA TTTGGCACTG 
AAACTTCTCC GAGATTATTC TGCAGATGAT ACCAGAAAAG TACACATGAT AACAGAGAAC 
ATCAATGCCT CTTGGGCAAG CATCCATAAA AGATTGAGTG AGCGAGAGGC TGCTCTGGAA 
GAAACCCACA GATTACTGCA ACAGTTCCCC TTGGACCTGG AGAAGTTCCT TGCCTGGCTT 
ACAGAAGCCG AAACAACTGC CAACGTCCTG CAGGATGCCA CCCATAAGGA AAGGCTTCTA 
GAAGATTCCA AGGGAGTAAG AGAGCTGATG AAACAATGGC AAGACCTCCA AGGAGAAATC 
GAAGCTCACA CAGATATCTA TCACAACCTG GACGAAAATG GCCAAAAAGT CCTGAGATCC 
CTGGAAGGTT CTGACGATGC AGCCTTGTTG CAAAGACGTT TGGATAACAT GAACTTCAAG 
TGGAGCGAAC TTCGGAAAAA GTCTCTCAAC ATTAGGTCTC ACTTGGAAGC CAGTTCTGAC 
CAGTGGAAGC GTCTGCACCT TTCTCTTCAG GAACTTCTGG TATGGCTCCA GCTGAAAGAT 
GATGAGTTAA GCCGGCAGGC ACCCATTGGA GGAGACTTTC CAGCGGTGCA GAAGCAGAAT 
GATGTACACA GGGCCTTCAA GAGGGAATTG AAAACGAAAG AACCTGTAAT CATGAGTACT 
CTTGAGACTG TACGAATATT TCTGACAGAG CAGCCTTTAG AAGGACTAGA GAAACTCTAC 
CAGGAGCCCA GAGAGCTGCC TCCTGAAGAG AGAGCCCAGA ATGTCACACG GCTCCTACGA 
AAGCAAGCTG AGGAGGTCAA CACTCAGTGG GAAAAACTGA ACGTGCACTC TGCAGACTGG 
CAGAGAAAAA TAGACGAGGC CCTCGAAAGA CTCCAGGAGC TTCAGGAAGC AACAGATGAG 
CTGGATCTCA AACTACGTCA GGCAGAGGTG ATCAAAGGAT CCTGGCAGCC TGTGGGTGAC 
CTCCTCATTG ACTCTCTCCA AGATCACCTC GAAAAAGTCA AGGCGCTTCG AGGAGAAATT 
ACACCTCTGA AAGAGAATGT CAGCTACGTC AATGACCTTG CTCGCCAACT CACTACGTTG 
GGCATTCAGC TGTCACCATA TAACCTCAAC ACTCTGGAAG ACCTGAACAC CAGATGGAAG 
CTTCTGCAGG TGGCCATTGA GGACCGCATC AGGCAGCTGC ATGAAGCGCA CAGGGACTTT 
GGACCAGCCT CCCAGCACTT CCTTTCCACT TCTGTCCAGG GTCCCTGGGA GAGAGCCATC 
TCACCAAACA AAGTGCCCTA CTATATCAAC CACGAGACCC AAACAACTTG CTGGGACCAT 
CCCAAAATGA CAGAGCTCTA CCAGTCTTTA GCTGACCTGA ATAATGTCAG GTTCTCAGCT 
TACAGGACTG CCATGAAACT CCGAAGACTG CAGAAGGCCC TTTGCTTGGA TCTCTTGAGC 
CTATCGGCTG CATGCGATGC CTTGGACCAG CACAACCTCA AGCAAAATGA CCAGCCCATG 
GATATCCTGC AGGTCATTAA CTGTCTGACC ACTATTTATG ATCGCCTAGA GCAAGAGCAC 
AACAATCTGG TCAACGTCCC TCTCTGCGTG GATATGTGTC TCAATTGGCT GCTGAATGTT 
TATGACACGG GACGAACGGG GAGGATCCGG GTCCTGTCTT TTAAAACTGG CATCATTTCT 
CTGTGTAAAG CCCATTTGGA AGACAAGTAC AGATACCTCT TCAAGCAAGT GGCAAGTTCG 
ACAGGATTTT GTGACCAGCG CAGGCTGGGC CTCCTCCTGC ATGACTCTAT CCAGATCCCA 
AGACAGTTGG GTGAAGTCGC ATCGTTCGGG GGCAGTAACA TTGAGCCGAG TGTCAGGAGC 
TGCTTCCAGT TTGCTAATAA TAAGCCTGAG ATCGAAGCGG CCCTCTTCCT AGACTGGATG 
CGCCTGGAGC CCCAGTCCAT GGTGTGGCTG CCTGTCCTGC ACCGAGTGGC TGCCGCGGAA 
ACTGCCAAGC ACCAGGCCAA GTGCAACATC TGCAAGGAGT GTCCCATCAT CGGATTCAGG 
TACAGGAGTC TAAAGCACTT TAATTATGAC ATCTGCCAAA GTTGCTTTTT TTCTGGTCGA 
GTTGCAAAAG GCCATAAAAT GCACTATCCC ATGGTGGAAT ACTGCACTCC GACTACATCG 
GGAGAAGATG TCCGTGACTT TGCCAAGGTA CTAAAAAACA AATTTCGAAC CAAAAGGTAT 
TTTGCGAAGC ATCCCCGAAT GGGCTACCTG CCAGTGCAGA CTGTCTTAGA GGGGGACAAC 
ATGGAAACTC CTGTCACTCT GATCAACTTC TGGCCGGTAG ATTCTGCGCC TGCCTCGTCC 
CCTCAGCTTT CACACGATGA TACTCATTCA CGCATTGAGC ATTATGCTAG CAGGCTAAAA 
AAAATGGAAA ACAGCAATGG ATCTTATCTA AATGATAGCA TCTCTCCTAA TGAGAGCATA 
GATGATGAAC ATTTGTTAAT CCAGCATTAC TGGCGAAGTT TGAACCAGGA ATCCCCCCTG 
AGCCAGCCTC GTAGTCCTGC CCAGATCTTG ATTTCCTTAG AGAGTGAGGA AAGAGGGGAG 
CTAGAGAGAA TCCTAGCAGA TCTTGAGGGG AGAAACAGAA ATCTGCAAGC AGAGTATGAT 
CGTCTAAAGC AGCAGCATGA ACACAAAGGC CTGTCCCCAC TGCCATCCCC TCCTGAAATG 
ATGCCTACTT CTCCCCAAAG TCCCCGGGAT GCTGAGCTCA TCGCTGAGGC CAAGCTGCTG 
CGTCAACACA AAGGCCGCCT GGAAGCCAGG ATGCAAATCC TTGAAGACCA TAACAAACAA 
CTGGAATCCC AGTTACACAG GCTCAGGCAG CTGCTGGAGC AACCCCAGGC AGAGGCCAAG 
GTGAATGGTA CAACGGTGTC TTCTCCTTCT ACCTCTCTTC AGAGGTCAGA TAGCAGTCAG 
CCTATGCTGC TCCGGGTGGT CGGCAGTCAG ACTTCAGAAT CCATGGGCGA GGAAGACCTG 
CTCAGCCCTC CCCAGGACAC AAGCACAGGG TTAGAGGAAG TGATGGAGCA GCTCAACCAC 
TCCTTCCCTA GTTCCAGAGG AAGAAATACC CCTGGGAAGC CAATGAGAGA GGACACAATG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMD ( XM_022415519.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022271227.1 Canis lupus familiaris dystrophin (DMD), transcript variant X9, mRNA.

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CloneID	OCe285078
Clone ID Related Accession (Same CDS sequence)	XM_022415519.1
Accession Version	XM_022415519.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	dystrophin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAATA TCATGGCTGG ATTGCAACAA ACCAACAGTG AAAAGATTCT CCTGAGCTGG 
GTCCGACAAT CAACTCGTAA TTATCCACAG GTTAATGTCA TTAACTTCAC CACCAGCTGG 
TCTGATGGCC TGGCTTTGAA CGCTCTCATC CACAGTCATA GGCCAGACCT GTTTGATTGG 
AATAGTGTGG TTTGCCAGCA GTCAGCCACA CAACGCCTGG AACATGCATT CAACATTGCC 
AAATATCAAT TAGGCATAGA GAAACTGCTT GATCCTGAAG ATGTTGCCAC CACTTATCCA 
GATAAGAAGT CCATCTTAAT GTATATCACA TCACTCTTCC AAGTTTTGCC TCAACAAGTG 
AGCATTGAAG CCATCCAGGA AGTGGAAATG TTGCCAAGGC CATCAAAAGT TACTAGAGAA 
GAACATTTTC AATTACATCA TCAAATGCAC TATTCTCAAC AGATCACAGT CAGTCTAGCA 
CAGGGCTATG AACGAGCCCC TTCCTCTCCT AAGCCTCGGT TCAAGAGCTA TGCCTACACA 
CAGGCTGCTT ATGTCACCAC TTCTGACCCC ACACGGAGCC CACTTCCTTC ACAGCATTTG 
GAAACTCCTG AAGACAAGTC ATTTGGCCGG TCATTGACAG AGACCGAAGC AAACCTGGAC 
AGTTATCAAA CAGCTTTGGA AGAAGTACTC TCGTGGCTTC TTTCAGCTGA GGATGCACTG 
CAAGCCCAAG GAGAGATTTC TAATGATGTC GAAGAAGTGA AAGAACAATT TCATACTCAT 
GAGGGATATA TGATGGACTT GACATCCCAT CAGGGACGGG TCGGTAATGT TCTCCAACTG 
GGAAGTCAAC TGATTGGAAC AGGGAAATTA TCAGAAGATG AAGAAACCGA AGTGCAGGAA 
CAAATGAATC TCCTCAATTC AAGATGGGAA TGCCTCAGGG TAGCTAGCAT GGAAAAACAA 
AGCAATTTAC ATAAAGTTCT AATGGATCTC CAGAATCAGC AACTGAAAGA GTTAAATGAC 
TGGCTAACCA AAACAGAAGA GAGAACAAGG AAAATGGAGA AGGAGCCCCT TGGACCTGAT 
ATTGAAGACC TAAAACGCCA AGTACAACAA CATAAGGTGC TTCAAGAAGA CTTAGAACAG 
GAACAAGTCA GGGTCAATTC CCTCACTCAT ATGGTGGTGG TAGTCGATGA ATCTAGTGGA 
GACCATGCAA CTGCTGCTTT GGAAGAACAA CTTAAGGTAC TGGGAGATCG ATGGGCAAAC 
ATCTGTAGGT GGACAGAAGA TCGCTGGGTT CTTTTACAAG ACATCCTCCT AAAATGGCAG 
CGTTTTACTG AAGAACAGTG CCTTTTCAGT GCATGGCTTT CGGAGAAGGA AGATGCAGTG 
AACAAGATTC ACACAACTGG CTTTAAGGAT CAAAGTGAAG TGTTATCAAA TCTTCAGAAA 
CTGGCTGTCT TAAAAACAGA TCTGGAAAAG AAGAAGCAAA CCATGGACAA ACTCTGCTCA 
CTCAACCAAG ACCTTCTTTC AGCGCTGAAA AACACAGTGG TAGCCCACAA GATGGAAGCA 
TGGCTGGACA ACTTTGCCCA GCGCTGGGAT AATTTAGTCC AGAAACTTGA AAAAAGTTCA 
GCACAGATTT CACAGGCTGT CACCACCACT CAGCCATCAC TAACACAGAC AACTGTAATG 
GAAACAGTAA CTATGGTGAC CACGAGGGAA CACATCTTGG TAAAGCATGC CCAAGAGGAA 
CTGCCACCAC CACCCCCTCA GAAGAAGAGG CAGATTATCG TGGATTCTGA AATTAGGAAA 
AGGTTGGATG TCGATATAAC TGAACTTCAC AGTTGGATTA CTCGTTCAGA AGCTGTGTTG 
CAGAGTCCTG AATTTGCAAT CTATCGGAAG GAAGGCAACT TCTCAGACCT TAAAGAAAAA 
GTCAATGCCA TAGAGCGAGA AAAAGCCGAG AAGTTCAGAA AACTGCAAGA TGCCAGCAGA 
TCAGCTCAGG CCCTGGTGGA ACAGATGGTG AATGAGGGTG TTAATGCTGA CAGCATCAAA 
CAAGCCTCCG AACAACTGAA CAGCCGGTGG ATAGAGTTCT GCCAATTGCT AAGCGAGAGA 
CTTAACTGGC TGGAGTATCA GAACAACATC ATCACTTTCT ATAATCAGCT ACAACAATTG 
GAGCAGATGA CAACTACTGC TGAAAACTGG TTGAAAACCC AGCCTACCAC CACATCAGAG 
CCAACAGCAA TTAAAAGCCA GTTAAAAATT TGTAAGGATG AAATCAACCG ACTGTCAGCT 
CTTCAGCCTC AAATCGAGCG ATTAAAAATT CAAAGCATAG CCCTGAAAGA GAAAGGACAA 
GGGCCAATGT TCCTGGATGC AGACTTTGTG GCCTTTACAA ATCATTTTAA CCAAGTCTTT 
GCTGATGTGC AGGCAAGAGA AAAAGAGCTA CAAACAATTT TTGACAGTTT GCCACCCATG 
CGCTATCAGG AGACTATGAG TACCATCCTG ACATGGATCC AGCAGTCAGA AACCAAACTC 
TCTATACCTC AGGTTACTGT CACTGAATAT GACATCATGG AACAGAGACT CGGAGAGCTA 
CAGGCTTTAC AAAGCTCTCT GCAAGAGCAA CAAAATGGCC TAAACTATCT CAGCACCACT 
GTGAAAGAGA TGTCAAAGAA AGCACCACTG TCTGATATTA GTCGGAAATA TCAATCAGAA 
TTTGAAGAGA TTGAGGGACG TTGGAAGAAG CTGTCTTCCC AGCTGGTTGA ACATTGTCAA 
AAGTTGGAGG AGCAAATGGC TAAACTTCGA AAAATTCAGA ATCACATAAA AACTCTGAAG 
AAATGGATCA CTGAAGTCGA TGTTTTCCTG AAGGAGGAAT GGCCTGCCCT TGGGGATTCA 
GAAATTCTGA AAAGACAGCT GAAACAGTGC AGGCTTTTAG TCAATGACAT TCAGACCATC 
CAGCCTAGTC TCAACAGTGT CAATGAAGGG GCTCAGAAGA TGAAGAATGA AGCAGAACCA 
GAGTTTGCTG GCAGACTTGA GACAGAGCTC CGAGAACTTA ACACCCAGTG GGATTACATG 
TGCCGCCAGG TCTATGCCAG GAAGGAAGCC TTAAAAGGAG GTTTGGATAA AACTGTAAGT 
CTTCAGAAAG ATCTGTCAGA GATGCATGAG TGGATGACAC AAGCTGAAGA AGAATACCTA 
GAGAGAGATT TCGAATACAA GACCCCTGAT GAATTACAGA CAGCAGTTGA AGAGATGAAG 
AGAGCTAAAG AAGAGGCCCA GCAAAAAGAA GCAAAAGTGA AACTCCTAAC CGAGTCCGTC 
AATAGTGTCA TAGCTCAGGC TCCACCTGCA GCACAAGAGG CCTTAAAAAA GGAACTTGAC 
ACTCTCACCA CCAACTACCA GTGGCTCTGC ACCAGGCTCA ATGGCAAATG CAAGACCTTG 
GAAGAAGTTT GGGCGTGCTG GCATGAGTTA TTGTCCTACT TGGAGAAGGC AAACAAGTGG 
CTAAGTGAAG TAGAAGTCAA GCTTAAAACC ACTGAAAATA TTTCTGGGGG AGCTGAGGAA 
ATCGCCGAGG TGCTTGATTC GCTTGAAAAT TTGATGCAAC ATTCAGAGGA TAACCCGAAT 
CAGATTCGCA TATTGGCACA GACCTTGACA GATGGTGGAG TCATGGATGA ACTGATCAAT 
GAGGAGCTTG AGACATTTAA TTCTCGTTGG AGAGAACTCC ATGAAGAGGC TGTGAGGAGG 
CAAAAGTTGC TTGAGCAGAG TATCCAGTCG GCCCAGGAGA TAGAAAAATC CTTGCACTTA 
ATTCAGGAGT CCCTCTCTTC CATTGACAAG CAGTTGGCAG CTTATATTGC TGACAAAGTG 
GATGCAGCTC AGATGCCTCA GGAAGCCCAG AAAATCCAAT CAGATTTGAC AAGTCATGAG 
ATCAGTTTAG AAGAAATGAA GAAACATAAC CAGGGAAAGG AGACTGCCCA AAGGGTACTA 
TCCCAAATTG ATGTGGCACA GAAAAAATTG CAGGATGTTT CCATGAAGTT TCGATTATTC 
CAGAAGCCAG CCAATTTTGA GCAGCGCCTA CAAGAAAGTA AAATGATTTT AGATGAAGTG 
AAGATGCATT TACCTGCGTT GGAAACAAAG AGTGTGGAAC AGGAAGTAGT ACAGTCACAG 
TTAAATCATT GTGTGAACTT GTATAAAAGT CTGAGTGAAG TGAAGTCTGA AGTGGAAATG 
GTAATAAAAA CTGGACGTCA GATTGTACAG AAGAAGCAGA CGGAAAACCC GAAAGAGCTT 
GATGAAAGAG TTACAGCTTT GAAATTGCAT TATAATGAGC TGGGAGCAAA GGTGACAGAA 
AGAAAGCAAC AGTTGGAAAA ATGCTTGAAA TTGTCCCGTA AGATGCGAAA GGAAATGAAT 
GCCCTGACAG AATGGCTGGC AGCGACAGAT ATGGAACTGA CGAAGAGATC GGCAGTTGAA 
GGAATGCCTA GTAATTTGGA TTCTGAAGTT GCCTGGGGAA AGGCTACTCA GAAAGAGATT 
GAGAAACAGA AGGTTCACCT AAAGAGTGTC ACAGAGGTAG GAGAGGCCTT GAAAACGGTT 
TTGGGCAAGA AGGAAATGTT GGTGGAAGAT AAACTGAGTC TTCTGAATAG TAACTGGATA 
GCCGTCACTT CCCGAGCAGA AGAGTGGTTA AACCTTTTAT TGGAATACCA GAAACACATG 
GAAAACTTTG ACCAGAATGT GGATTACATC ACAAACTGGA TCATTCAGGC TGATGCACTT 
TTGGATGAAT CTGAGAAAAA GAAACCTCAG CAAAAAGAAG ACATACTTAA GCGTTTAAAG 
GCTGAAATGA ATGACATACG TCCAAAGGTG GATTCTACAC GTGACCAAGC AGCAAACCTG 
ATGGCAAACC GCGGCGACCA CTGCAGGAAA GTAGTAGAGC CCAAAATCTC AGAGCTCAAC 
CATCGATTTG CAGCCATTTC TCACAGAATT AAGACTGGAA AGGCCTCCAT TCCTTTGAAG 
GAATTGGAGC AGTTTAACTC AGATATACAA AAATTGCTTG AACCACTGGA GGCTGAAATT 
CAGCAGGGGG TGAATCTGAA AGAGGAAGAC TTCAATAAAG ATATGAGTGA AGACAATGAG 
GGTACTGTAA AAGAATTGTT GCAAAGAGGA GACAACTTAC AACAAAGAAT CACAGATGAG 
AGAAAGCGAG AGGAAATAAA GATAAAACAA CAGCTGTTAC AGACAAAACA TAATGCTCTC 
AAGGATTTGA GGTCTCAAAG AAGAAAAAAG GCTCTAGAAA TTTCTCACCA GTGGTATCAG 
TACAAGAGGC AGGCTGATGA TCTCCTGAAA TGCTTGGATG ACATTGAAAA AAAATTAGCC 
AGCCTACCTG AACCCAGAGA TGAAAGGAAA ATAAAGGAAA TTGATCGTGA ATTGCAGAAG 
AAGAAAGAGG AGCTGAATGC AGTGCGTAGG CAAGCTGAGG GCTTGTCTGA GGATGGGGCC 
GCAATGGCAG TGGAGCCAAC TCAGATCCAG CTCAGCAAGC GCTGGCGGGA AATTGAGAGC 
AAATTTGCTC AGTTTCGAAG ACTCAACTTT GCACAAATTC ACACTGTCCA TGAAGAGTCA 
GTGGTGGCGA TGACTGAAGA CATGCCTTTG GAAATTTCTT ATGTGCCTTC TACTTACCTG 
ACTGAGATCA CTCATGTCTC ACAAGCCCTA TCAGAAGTGG AAGAGCTTCT TAATGCTCCC 
GACCTTTGTG CTCAAGATTT TGAAGATCTC TTTAAACAAG AGGAATCCTT GAAGAACATA 
AAAGACAGCC TGCAACAAAT CTCAGGTCGG ATTGACATCA TTCACAATAA AAAGACAGCA 
GCATTGCACA GTGCCACTCC TGCAGAAAGG GCAAAGCTCC AGGAAGCTCT CTCACGGCTT 
GATTTCCAAT GGGAAAGAGT TAACAATATG TACAAGGACC GACAAGGGAG ATTTGACAGA 
TCTGTGGAAA AATGGCGGCG GTTTCATTAT GATATGAAGA TACTTAATCA ATGGCTAACA 
GAAGCTGAAC AGTTTCTCAA AAAGACACAA ATTCCTGAGA ATTGGGAACA TGCCAAATAC 
AAATGGTATC TTAAGGAACT CCAGGATGGC ATTGGACAGC GGCAAAGTGT TGTCAGGGTA 
TTGAATGCAA CTGGGGAAGA AATAATTCAA CAGTCCTCAA AAACAGATGC CAGTATTCTC 
CAAGAAAAAC TGGGAAGCCT GAATCTGCGG TGGCAGGAGG TCTGCAAACA GCTGGCAGAA 
AGAAAAAAGA GGCTAGAGGA ACAGAAGAAT ATCTTGTCAG AATTTCAAAG AGATGTAAAT 
GAATTTGTTT TATGGTTGGA AGAAGCGGAT AACGTTGCTA ATATTCCACT TGAACCTGGA 
AATGAGCAGC AGCTAAAAGA AAAACTTGAA CAAGTCAAGT TACTGGCAGA AGAGTTGCCC 
CTGCGCCAGG GAATTCTAAA ACAATTAAAT GAAACTGGAG GAACAGTGCT TGTAAGTGCT 
CCCCTAAGCC CAGAAGAGCA AGATAAACTT GAAAATAAGC TCAAGCAGAC AAATCTTCAG 
TGGATAAAGG TTTCTAGAAA TCTGCCTGAG AAGCAAGAAG AAATTGAGGC ACACGTAAAA 
GACCTTGGAC AGCTGGAAGA GCAGTTAAAT CATCTGCTTC TATGGCTGTC TCCTATTAGG 
AATCAGTTGG AAATTTACAA TCAGCCAAAT CAAACAGGAC CATTTGACAT CAAGGAAATT 
GAAGTAGCAG TTCAAGCTAA ACAGCCGGAT GTGGAAGGGA TTTTGTCTAA AGGGCAGCAT 
TTGTACAAGG AAAAACCAGC CACTCAGCCA GCGAAGAGAA AGCTGGAAGA TCTCAGCTCT 
GATTGGAAGG TGGTAACTCA GTTGCTTCAA GAGCTGCGGG CAAAGCAGCC TGGCCCAGCT 
CCTGGACTGA CCACTGTCAG AGCCCCTCCC AGTCAGACTG TTACTCTGGT GACACAACCC 
GCGGTTACCA AGGAAACTGC CATCTCCAAA CTAGAAATGC CATCTTCATT GCTGTTGGAG 
GTACCTGCAC TGGCAGATTT CAACCGAGCT TGGACAGAAC TTACCGACTG GCTGTCTCTG 
CTTGATCGAG TTATAAAATC ACAGAGGGTG ATGGTGGGTG ATCTTGAAGA CATTAACGAG 
ATGATCATCA AGCAGAAGGC AACGCTGCAG GATTTGGAAC AGAGGCGCCC CCAGTTGGAA 
GAACTCATTA CCGCTGCCCA GAATTTGAAA AACAAGACCA GCAATCAAGA GGCTAGAACA 
ATCATTACTG ATCGAATTGA AAGAATTCAG AGTCAGTGGG ATGAAGTACA GGAACATCTT 
CAGAACCGGA GGCAACAGTT GAATGAAATG TTAAAGGATT CCACACAATG GCTGGAAGCT 
AAAGAGGAGG CTGAGCAGGT GTTGGGGCAG GCCAGAGCCA AGCTTGAGTC ATGGAAGGAG 
GCTCCCTACA CAGTAGATGC AATCCAAAAG AAAATCACAG AAACCAAGCA GTTGGCCAAA 
GACCTCCGCC AGTGGCAGAT AAATGTAGAT GTTGCAAATG ATTTGGCACT GAAACTTCTC 
CGAGATTATT CTGCAGATGA TACCAGAAAA GTACACATGA TAACAGAGAA CATCAATGCC 
TCTTGGGCAA GCATCCATAA AAGATTGAGT GAGCGAGAGG CTGCTCTGGA AGAAACCCAC 
AGATTACTGC AACAGTTCCC CTTGGACCTG GAGAAGTTCC TTGCCTGGCT TACAGAAGCC 
GAAACAACTG CCAACGTCCT GCAGGATGCC ACCCATAAGG AAAGGCTTCT AGAAGATTCC 
AAGGGAGTAA GAGAGCTGAT GAAACAATGG CAAGATCTCC AAGGAGAAAT CGAAGCTCAC 
ACAGATATCT ATCACAACCT GGACGAAAAT GGCCAAAAAG TCCTGAGATC CCTGGAAGGT 
TCTGACGATG CAGCCTTGTT GCAAAGACGT TTGGATAACA TGAACTTCAA GTGGAGCGAA 
CTTCGGAAAA AGTCTCTCAA CATTAGGTCT CACTTGGAAG CCAGTTCTGA CCAGTGGAAG 
CGTCTGCACC TTTCTCTTCA GGAACTTCTG GTATGGCTCC AGCTGAAAGA TGATGAGTTA 
AGCCGGCAGG CACCCATTGG AGGAGACTTT CCAGCGGTGC AGAAGCAGAA TGATGTACAC 
AGGGCCTTCA AGAGGGAATT GAAAACGAAA GAACCTGTAA TCATGAGTAC TCTTGAGACT 
GTACGAATAT TTCTGACAGA GCAGCCTTTA GAAGGACTAG AGAAACTCTA CCAGGAGCCC 
AGAGAGCTGC CTCCTGAAGA GAGAGCCCAG AATGTCACAC GGCTCCTACG AAAGCAAGCT 
GAGGAGGTCA ACACTCAGTG GGAAAAACTG AACGTGCACT CTGCAGACTG GCAGAGAAAA 
ATAGACGAGG CCCTCGAAAG ACTCCAGGAG CTTCAGGAAG CAACAGATGA GCTGGATCTC 
AAACTACGTC AGGCAGAGGT GATCAAAGGA TCCTGGCAGC CTGTGGGTGA TCTCCTCATT 
GACTCTCTCC AAGATCACCT CGAAAAAGTC AAGGCGCTTC GAGGAGAAAT TACACCTCTG 
AAAGAGAATG TCAGCTACGT CAATGACCTT GCTCGCCAAC TCACTACGTT GGGCATTCAG 
CTGTCACCAT ATAACCTCAA CACTCTGGAA GACCTGAACA CCAGATGGAA GCTTCTGCAG 
GTGGCCATTG AGGACCGCAT CAGGCAGCTG CATGAAGCGC ACAGGGACTT TGGACCAGCC 
TCCCAGCACT TCCTTTCCAC TTCTGTCCAG GGTCCCTGGG AGAGAGCCAT CTCACCAAAC 
AAAGTGCCCT ACTATATCAA CCACGAGACC CAAACAACTT GCTGGGACCA TCCCAAAATG 
ACAGAGCTCT ACCAGTCTTT AGCTGACCTG AATAATGTCA GGTTCTCAGC TTACAGGACT 
GCCATGAAAC TCCGAAGACT GCAGAAGGCC CTTTGCTTGG ATCTCTTGAG CCTATCGGCT 
GCATGCGATG CCTTGGACCA GCACAACCTC AAGCAAAATG ACCAGCCCAT GGATATCCTG 
CAGGTCATTA ACTGTCTGAC CACTATTTAT GATCGCCTAG AGCAAGAGCA CAACAATCTG 
GTCAACGTCC CTCTCTGCGT GGATATGTGT CTCAATTGGC TGCTGAATGT TTATGACACG 
GGACGAACGG GGAGGATCCG GGTCCTGTCT TTTAAAACTG GCATCATTTC TCTGTGTAAA 
GCCCATTTGG AAGACAAGTA CAGATACCTC TTCAAGCAAG TGGCAAGTTC GACAGGATTT 
TGTGACCAGC GCAGGCTGGG CCTCCTCCTG CATGACTCTA TCCAGATCCC AAGACAGTTG 
GGTGAAGTCG CATCGTTCGG GGGCAGTAAC ATTGAGCCGA GTGTCAGGAG CTGCTTCCAG 
TTTGCTAATA ATAAGCCTGA GATCGAAGCG GCCCTCTTCC TAGACTGGAT GCGCCTGGAG 
CCCCAGTCCA TGGTGTGGCT GCCTGTCCTG CACCGAGTGG CTGCCGCGGA AACTGCCAAG 
CACCAGGCCA AGTGCAACAT CTGCAAGGAG TGTCCCATCA TCGGATTCAG GTACAGGAGT 
CTAAAGCACT TTAATTATGA CATCTGCCAA AGTTGCTTTT TTTCTGGTCG AGTTGCAAAA 
GGCCATAAAA TGCACTATCC CATGGTGGAA TACTGCACTC CGACTACATC GGGAGAAGAT 
GTCCGTGACT TTGCCAAGGT ACTAAAAAAC AAATTTCGAA CCAAAAGGTA TTTTGCGAAG 
CATCCCCGAA TGGGCTACCT GCCAGTGCAG ACTGTCTTAG AGGGGGACAA CATGGAAACT 
CCTGTCACTC TGATCAACTT CTGGCCGGTA GATTCTGCGC CTGCCTCGTC CCCTCAGCTT 
TCACACGATG ATACTCATTC ACGCATTGAG CATTATGCTA GCAGGCTAGC AGAAATGGAA 
AACAGCAATG GATCTTATCT AAATGATAGC ATCTCTCCTA ATGAGAGCAT AGATGATGAA 
CATTTGTTAA TCCAGCATTA CTGCCAAAGT TTGAACCAGG ACTCCCCCCT GAGCCAGCCT 
CGTAGTCCTG CCCAGATCTT GATTTCCTTA GAGAGTGAGG AAAGAGGGGA GCTAGAGAGA 
ATCCTAGCAG ATCTTGAGGA AGAAAACAGA AATCTGCAAG CAGAGTATGA TCGTCTAAAG 
CAGCAGCATG AACACAAAGG CCTGTCCCCA CTGCCATCCC CTCCTGAAAT GATGCCTACT 
TCTCCCCAAA GTCCCCGGGA TGCTGAGCTC ATCGCTGAGG CCAAGCTGCT GCGTCAACAC 
AAAGGCCGCC TGGAAGCCAG GATGCAAATC CTTGAAGACC ATAACAAACA ACTGGAATCC 
CAGTTACACA GGCTCAGGCA GCTGCTGGAG CAACCCCAGG CAGAGGCCAA GGTGAATGGT 
ACAACGGTGT CTTCTCCTTC TACCTCTCTT CAGAGGTCAG ATAGCAGTCA GCCTATGCTG 
CTCCGGGTGG TCGGCAGTCA GACTTCAGAA TCCATGGGCG AGGAAGACCT GCTCAGCCCT 
CCCCAGGACA CAAGCACAGG GTTAGAGGAA GTGATGGAGC AGCTCAACCA CTCCTTCCCT 
AGTTCCAGAG GACACAATGT AGGAAGCCTT TTCCACTTGG CAGATGATTT GGGCAGAGCG 
ATGGAGTCCT TAGTTTCAGT CATGACAGAT GAAGAAGGGG CAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022271227.1
CDS	32..10759
Translation	MKNIMAGLQQTNSEKILLSWVRQSTRNYPQVNVINFTTSWSDGLALNALIHSHRPDLFDWNSVVCQQSATQRLEHAFNIAKYQLGIEKLLDPEDVATTYPDKKSILMYITSLFQVLPQQVSIEAIQEVEMLPRPSKVTREEHFQLHHQMHYSQQITVSLAQGYERAPSSPKPRFKSYAYTQAAYVTTSDPTRSPLPSQHLETPEDKSFGRSLTETEANLDSYQTALEEVLSWLLSAEDALQAQGEISNDVEEVKEQFHTHEGYMMDLTSHQGRVGNVLQLGSQLIGTGKLSEDEETEVQEQMNLLNSRWECLRVASMEKQSNLHKVLMDLQNQQLKELNDWLTKTEERTRKMEKEPLGPDIEDLKRQVQQHKVLQEDLEQEQVRVNSLTHMVVVVDESSGDHATAALEEQLKVLGDRWANICRWTEDRWVLLQDILLKWQRFTEEQCLFSAWLSEKEDAVNKIHTTGFKDQSEVLSNLQKLAVLKTDLEKKKQTMDKLCSLNQDLLSALKNTVVAHKMEAWLDNFAQRWDNLVQKLEKSSAQISQAVTTTQPSLTQTTVMETVTMVTTREHILVKHAQEELPPPPPQKKRQIIVDSEIRKRLDVDITELHSWITRSEAVLQSPEFAIYRKEGNFSDLKEKVNAIEREKAEKFRKLQDASRSAQALVEQMVNEGVNADSIKQASEQLNSRWIEFCQLLSERLNWLEYQNNIITFYNQLQQLEQMTTTAENWLKTQPTTTSEPTAIKSQLKICKDEINRLSALQPQIERLKIQSIALKEKGQGPMFLDADFVAFTNHFNQVFADVQAREKELQTIFDSLPPMRYQETMSTILTWIQQSETKLSIPQVTVTEYDIMEQRLGELQALQSSLQEQQNGLNYLSTTVKEMSKKAPLSDISRKYQSEFEEIEGRWKKLSSQLVEHCQKLEEQMAKLRKIQNHIKTLKKWITEVDVFLKEEWPALGDSEILKRQLKQCRLLVNDIQTIQPSLNSVNEGAQKMKNEAEPEFAGRLETELRELNTQWDYMCRQVYARKEALKGGLDKTVSLQKDLSEMHEWMTQAEEEYLERDFEYKTPDELQTAVEEMKRAKEEAQQKEAKVKLLTESVNSVIAQAPPAAQEALKKELDTLTTNYQWLCTRLNGKCKTLEEVWACWHELLSYLEKANKWLSEVEVKLKTTENISGGAEEIAEVLDSLENLMQHSEDNPNQIRILAQTLTDGGVMDELINEELETFNSRWRELHEEAVRRQKLLEQSIQSAQEIEKSLHLIQESLSSIDKQLAAYIADKVDAAQMPQEAQKIQSDLTSHEISLEEMKKHNQGKETAQRVLSQIDVAQKKLQDVSMKFRLFQKPANFEQRLQESKMILDEVKMHLPALETKSVEQEVVQSQLNHCVNLYKSLSEVKSEVEMVIKTGRQIVQKKQTENPKELDERVTALKLHYNELGAKVTERKQQLEKCLKLSRKMRKEMNALTEWLAATDMELTKRSAVEGMPSNLDSEVAWGKATQKEIEKQKVHLKSVTEVGEALKTVLGKKEMLVEDKLSLLNSNWIAVTSRAEEWLNLLLEYQKHMENFDQNVDYITNWIIQADALLDESEKKKPQQKEDILKRLKAEMNDIRPKVDSTRDQAANLMANRGDHCRKVVEPKISELNHRFAAISHRIKTGKASIPLKELEQFNSDIQKLLEPLEAEIQQGVNLKEEDFNKDMSEDNEGTVKELLQRGDNLQQRITDERKREEIKIKQQLLQTKHNALKDLRSQRRKKALEISHQWYQYKRQADDLLKCLDDIEKKLASLPEPRDERKIKEIDRELQKKKEELNAVRRQAEGLSEDGAAMAVEPTQIQLSKRWREIESKFAQFRRLNFAQIHTVHEESVVAMTEDMPLEISYVPSTYLTEITHVSQALSEVEELLNAPDLCAQDFEDLFKQEESLKNIKDSLQQISGRIDIIHNKKTAALHSATPAERAKLQEALSRLDFQWERVNNMYKDRQGRFDRSVEKWRRFHYDMKILNQWLTEAEQFLKKTQIPENWEHAKYKWYLKELQDGIGQRQSVVRVLNATGEEIIQQSSKTDASILQEKLGSLNLRWQEVCKQLAERKKRLEEQKNILSEFQRDVNEFVLWLEEADNVANIPLEPGNEQQLKEKLEQVKLLAEELPLRQGILKQLNETGGTVLVSAPLSPEEQDKLENKLKQTNLQWIKVSRNLPEKQEEIEAHVKDLGQLEEQLNHLLLWLSPIRNQLEIYNQPNQTGPFDIKEIEVAVQAKQPDVEGILSKGQHLYKEKPATQPAKRKLEDLSSDWKVVTQLLQELRAKQPGPAPGLTTVRAPPSQTVTLVTQPAVTKETAISKLEMPSSLLLEVPALADFNRAWTELTDWLSLLDRVIKSQRVMVGDLEDINEMIIKQKATLQDLEQRRPQLEELITAAQNLKNKTSNQEARTIITDRIERIQSQWDEVQEHLQNRRQQLNEMLKDSTQWLEAKEEAEQVLGQARAKLESWKEAPYTVDAIQKKITETKQLAKDLRQWQINVDVANDLALKLLRDYSADDTRKVHMITENINASWASIHKRLSEREAALEETHRLLQQFPLDLEKFLAWLTEAETTANVLQDATHKERLLEDSKGVRELMKQWQDLQGEIEAHTDIYHNLDENGQKVLRSLEGSDDAALLQRRLDNMNFKWSELRKKSLNIRSHLEASSDQWKRLHLSLQELLVWLQLKDDELSRQAPIGGDFPAVQKQNDVHRAFKRELKTKEPVIMSTLETVRIFLTEQPLEGLEKLYQEPRELPPEERAQNVTRLLRKQAEEVNTQWEKLNVHSADWQRKIDEALERLQELQEATDELDLKLRQAEVIKGSWQPVGDLLIDSLQDHLEKVKALRGEITPLKENVSYVNDLARQLTTLGIQLSPYNLNTLEDLNTRWKLLQVAIEDRIRQLHEAHRDFGPASQHFLSTSVQGPWERAISPNKVPYYINHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQVINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPVTLINFWPVDSAPASSPQLSHDDTHSRIEHYASRLAEMENSNGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKQQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDTSTGLEEVMEQLNHSFPSSRGHNVGSLFHLADDLGRAMESLVSVMTDEEGAE

Target ORF information:

RefSeq Version	XM_022415519.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris dystrophin (DMD), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022415519.1

ORF Insert Sequence:

ATGAAAAATA TCATGGCTGG ATTGCAACAA ACCAACAGTG AAAAGATTCT CCTGAGCTGG 
GTCCGACAAT CAACTCGTAA TTATCCACAG GTTAATGTCA TTAACTTCAC CACCAGCTGG 
TCTGATGGCC TGGCTTTGAA CGCTCTCATC CACAGTCATA GGCCAGACCT GTTTGATTGG 
AATAGTGTGG TTTGCCAGCA GTCAGCCACA CAACGCCTGG AACATGCATT CAACATTGCC 
AAATATCAAT TAGGCATAGA GAAACTGCTT GATCCTGAAG ATGTTGCCAC CACTTATCCA 
GATAAGAAGT CCATCTTAAT GTATATCACA TCACTCTTCC AAGTTTTGCC TCAACAAGTG 
AGCATTGAAG CCATCCAGGA AGTGGAAATG TTGCCAAGGC CATCAAAAGT TACTAGAGAA 
GAACATTTTC AATTACATCA TCAAATGCAC TATTCTCAAC AGATCACAGT CAGTCTAGCA 
CAGGGCTATG AACGAGCCCC TTCCTCTCCT AAGCCTCGGT TCAAGAGCTA TGCCTACACA 
CAGGCTGCTT ATGTCACCAC TTCTGACCCC ACACGGAGCC CACTTCCTTC ACAGCATTTG 
GAAACTCCTG AAGACAAGTC ATTTGGCCGG TCATTGACAG AGACCGAAGC AAACCTGGAC 
AGTTATCAAA CAGCTTTGGA AGAAGTACTC TCGTGGCTTC TTTCAGCTGA GGATGCACTG 
CAAGCCCAAG GAGAGATTTC TAATGATGTC GAAGAAGTGA AAGAACAATT TCATACTCAT 
GAGGGATATA TGATGGACTT GACATCCCAT CAGGGACGGG TCGGTAATGT TCTCCAACTG 
GGAAGTCAAC TGATTGGAAC AGGGAAATTA TCAGAAGATG AAGAAACCGA AGTGCAGGAA 
CAAATGAATC TCCTCAATTC AAGATGGGAA TGCCTCAGGG TAGCTAGCAT GGAAAAACAA 
AGCAATTTAC ATAAAGTTCT AATGGATCTC CAGAATCAGC AACTGAAAGA GTTAAATGAC 
TGGCTAACCA AAACAGAAGA GAGAACAAGG AAAATGGAGA AGGAGCCCCT TGGACCTGAT 
ATTGAAGACC TAAAACGCCA AGTACAACAA CATAAGGTGC TTCAAGAAGA CTTAGAACAG 
GAACAAGTCA GGGTCAATTC CCTCACTCAT ATGGTGGTGG TAGTCGATGA ATCTAGTGGA 
GACCATGCAA CTGCTGCTTT GGAAGAACAA CTTAAGGTAC TGGGAGATCG ATGGGCAAAC 
ATCTGTAGGT GGACAGAAGA TCGCTGGGTT CTTTTACAAG ACATCCTCCT AAAATGGCAG 
CGTTTTACTG AAGAACAGTG CCTTTTCAGT GCATGGCTTT CGGAGAAGGA AGATGCAGTG 
AACAAGATTC ACACAACTGG CTTTAAGGAT CAAAGTGAAG TGTTATCAAA TCTTCAGAAA 
CTGGCTGTCT TAAAAACAGA TCTGGAAAAG AAGAAGCAAA CCATGGACAA ACTCTGCTCA 
CTCAACCAAG ACCTTCTTTC AGCGCTGAAA AACACAGTGG TAGCCCACAA GATGGAAGCA 
TGGCTGGACA ACTTTGCCCA GCGCTGGGAT AATTTAGTCC AGAAACTTGA AAAAAGTTCA 
GCACAGATTT CACAGGCTGT CACCACCACT CAGCCATCAC TAACACAGAC AACTGTAATG 
GAAACAGTAA CTATGGTGAC CACGAGGGAA CACATCTTGG TAAAGCATGC CCAAGAGGAA 
CTGCCACCAC CACCCCCTCA GAAGAAGAGG CAGATTATCG TGGATTCTGA AATTAGGAAA 
AGGTTGGATG TCGATATAAC TGAACTTCAC AGTTGGATTA CTCGTTCAGA AGCTGTGTTG 
CAGAGTCCTG AATTTGCAAT CTATCGGAAG GAAGGCAACT TCTCAGACCT TAAAGAAAAA 
GTCAATGCCA TAGAGCGAGA AAAAGCCGAG AAGTTCAGAA AACTGCAAGA TGCCAGCAGA 
TCAGCTCAGG CCCTGGTGGA ACAGATGGTG AATGAGGGTG TTAATGCTGA CAGCATCAAA 
CAAGCCTCCG AACAACTGAA CAGCCGGTGG ATAGAGTTCT GCCAATTGCT AAGCGAGAGA 
CTTAACTGGC TGGAGTATCA GAACAACATC ATCACTTTCT ATAATCAGCT ACAACAATTG 
GAGCAGATGA CAACTACTGC TGAAAACTGG TTGAAAACCC AGCCTACCAC CACATCAGAG 
CCAACAGCAA TTAAAAGCCA GTTAAAAATT TGTAAGGATG AAATCAACCG ACTGTCAGCT 
CTTCAGCCTC AAATCGAGCG ATTAAAAATT CAAAGCATAG CCCTGAAAGA GAAAGGACAA 
GGGCCAATGT TCCTGGATGC AGACTTTGTG GCCTTTACAA ATCATTTTAA CCAAGTCTTT 
GCTGATGTGC AGGCAAGAGA AAAAGAGCTA CAAACAATTT TTGACAGTTT GCCACCCATG 
CGCTATCAGG AGACTATGAG TACCATCCTG ACATGGATCC AGCAGTCAGA AACCAAACTC 
TCTATACCTC AGGTTACTGT CACTGAATAT GACATCATGG AACAGAGACT CGGAGAGCTA 
CAGGCTTTAC AAAGCTCTCT GCAAGAGCAA CAAAATGGCC TAAACTATCT CAGCACCACT 
GTGAAAGAGA TGTCAAAGAA AGCACCACTG TCTGATATTA GTCGGAAATA TCAATCAGAA 
TTTGAAGAGA TTGAGGGACG TTGGAAGAAG CTGTCTTCCC AGCTGGTTGA ACATTGTCAA 
AAGTTGGAGG AGCAAATGGC TAAACTTCGA AAAATTCAGA ATCACATAAA AACTCTGAAG 
AAATGGATCA CTGAAGTCGA TGTTTTCCTG AAGGAGGAAT GGCCTGCCCT TGGGGATTCA 
GAAATTCTGA AAAGACAGCT GAAACAGTGC AGGCTTTTAG TCAATGACAT TCAGACCATC 
CAGCCTAGTC TCAACAGTGT CAATGAAGGG GCTCAGAAGA TGAAGAATGA AGCAGAACCA 
GAGTTTGCTG GCAGACTTGA GACAGAGCTC CGAGAACTTA ACACCCAGTG GGATTACATG 
TGCCGCCAGG TCTATGCCAG GAAGGAAGCC TTAAAAGGAG GTTTGGATAA AACTGTAAGT 
CTTCAGAAAG ATCTGTCAGA GATGCATGAG TGGATGACAC AAGCTGAAGA AGAATACCTA 
GAGAGAGATT TCGAATACAA GACCCCTGAT GAATTACAGA CAGCAGTTGA AGAGATGAAG 
AGAGCTAAAG AAGAGGCCCA GCAAAAAGAA GCAAAAGTGA AACTCCTAAC CGAGTCCGTC 
AATAGTGTCA TAGCTCAGGC TCCACCTGCA GCACAAGAGG CCTTAAAAAA GGAACTTGAC 
ACTCTCACCA CCAACTACCA GTGGCTCTGC ACCAGGCTCA ATGGCAAATG CAAGACCTTG 
GAAGAAGTTT GGGCGTGCTG GCATGAGTTA TTGTCCTACT TGGAGAAGGC AAACAAGTGG 
CTAAGTGAAG TAGAAGTCAA GCTTAAAACC ACTGAAAATA TTTCTGGGGG AGCTGAGGAA 
ATCGCCGAGG TGCTTGATTC GCTTGAAAAT TTGATGCAAC ATTCAGAGGA TAACCCGAAT 
CAGATTCGCA TATTGGCACA GACCTTGACA GATGGTGGAG TCATGGATGA ACTGATCAAT 
GAGGAGCTTG AGACATTTAA TTCTCGTTGG AGAGAACTCC ATGAAGAGGC TGTGAGGAGG 
CAAAAGTTGC TTGAGCAGAG TATCCAGTCG GCCCAGGAGA TAGAAAAATC CTTGCACTTA 
ATTCAGGAGT CCCTCTCTTC CATTGACAAG CAGTTGGCAG CTTATATTGC TGACAAAGTG 
GATGCAGCTC AGATGCCTCA GGAAGCCCAG AAAATCCAAT CAGATTTGAC AAGTCATGAG 
ATCAGTTTAG AAGAAATGAA GAAACATAAC CAGGGAAAGG AGACTGCCCA AAGGGTACTA 
TCCCAAATTG ATGTGGCACA GAAAAAATTG CAGGATGTTT CCATGAAGTT TCGATTATTC 
CAGAAGCCAG CCAATTTTGA GCAGCGCCTA CAAGAAAGTA AAATGATTTT AGATGAAGTG 
AAGATGCATT TACCTGCGTT GGAAACAAAG AGTGTGGAAC AGGAAGTAGT ACAGTCACAG 
TTAAATCATT GTGTGAACTT GTATAAAAGT CTGAGTGAAG TGAAGTCTGA AGTGGAAATG 
GTAATAAAAA CTGGACGTCA GATTGTACAG AAGAAGCAGA CGGAAAACCC GAAAGAGCTT 
GATGAAAGAG TTACAGCTTT GAAATTGCAT TATAATGAGC TGGGAGCAAA GGTGACAGAA 
AGAAAGCAAC AGTTGGAAAA ATGCTTGAAA TTGTCCCGTA AGATGCGAAA GGAAATGAAT 
GCCCTGACAG AATGGCTGGC AGCGACAGAT ATGGAACTGA CGAAGAGATC GGCAGTTGAA 
GGAATGCCTA GTAATTTGGA TTCTGAAGTT GCCTGGGGAA AGGCTACTCA GAAAGAGATT 
GAGAAACAGA AGGTTCACCT AAAGAGTGTC ACAGAGGTAG GAGAGGCCTT GAAAACGGTT 
TTGGGCAAGA AGGAAATGTT GGTGGAAGAT AAACTGAGTC TTCTGAATAG TAACTGGATA 
GCCGTCACTT CCCGAGCAGA AGAGTGGTTA AACCTTTTAT TGGAATACCA GAAACACATG 
GAAAACTTTG ACCAGAATGT GGATTACATC ACAAACTGGA TCATTCAGGC TGATGCACTT 
TTGGATGAAT CTGAGAAAAA GAAACCTCAG CAAAAAGAAG ACATACTTAA GCGTTTAAAG 
GCTGAAATGA ATGACATACG TCCAAAGGTG GATTCTACAC GTGACCAAGC AGCAAACCTG 
ATGGCAAACC GCGGCGACCA CTGCAGGAAA GTAGTAGAGC CCAAAATCTC AGAGCTCAAC 
CATCGATTTG CAGCCATTTC TCACAGAATT AAGACTGGAA AGGCCTCCAT TCCTTTGAAG 
GAATTGGAGC AGTTTAACTC AGATATACAA AAATTGCTTG AACCACTGGA GGCTGAAATT 
CAGCAGGGGG TGAATCTGAA AGAGGAAGAC TTCAATAAAG ATATGAGTGA AGACAATGAG 
GGTACTGTAA AAGAATTGTT GCAAAGAGGA GACAACTTAC AACAAAGAAT CACAGATGAG 
AGAAAGCGAG AGGAAATAAA GATAAAACAA CAGCTGTTAC AGACAAAACA TAATGCTCTC 
AAGGATTTGA GGTCTCAAAG AAGAAAAAAG GCTCTAGAAA TTTCTCACCA GTGGTATCAG 
TACAAGAGGC AGGCTGATGA TCTCCTGAAA TGCTTGGATG ACATTGAAAA AAAATTAGCC 
AGCCTACCTG AACCCAGAGA TGAAAGGAAA ATAAAGGAAA TTGATCGTGA ATTGCAGAAG 
AAGAAAGAGG AGCTGAATGC AGTGCGTAGG CAAGCTGAGG GCTTGTCTGA GGATGGGGCC 
GCAATGGCAG TGGAGCCAAC TCAGATCCAG CTCAGCAAGC GCTGGCGGGA AATTGAGAGC 
AAATTTGCTC AGTTTCGAAG ACTCAACTTT GCACAAATTC ACACTGTCCA TGAAGAGTCA 
GTGGTGGCGA TGACTGAAGA CATGCCTTTG GAAATTTCTT ATGTGCCTTC TACTTACCTG 
ACTGAGATCA CTCATGTCTC ACAAGCCCTA TCAGAAGTGG AAGAGCTTCT TAATGCTCCC 
GACCTTTGTG CTCAAGATTT TGAAGATCTC TTTAAACAAG AGGAATCCTT GAAGAACATA 
AAAGACAGCC TGCAACAAAT CTCAGGTCGG ATTGACATCA TTCACAATAA AAAGACAGCA 
GCATTGCACA GTGCCACTCC TGCAGAAAGG GCAAAGCTCC AGGAAGCTCT CTCACGGCTT 
GATTTCCAAT GGGAAAGAGT TAACAATATG TACAAGGACC GACAAGGGAG ATTTGACAGA 
TCTGTGGAAA AATGGCGGCG GTTTCATTAT GATATGAAGA TACTTAATCA ATGGCTAACA 
GAAGCTGAAC AGTTTCTCAA AAAGACACAA ATTCCTGAGA ATTGGGAACA TGCCAAATAC 
AAATGGTATC TTAAGGAACT CCAGGATGGC ATTGGACAGC GGCAAAGTGT TGTCAGGGTA 
TTGAATGCAA CTGGGGAAGA AATAATTCAA CAGTCCTCAA AAACAGATGC CAGTATTCTC 
CAAGAAAAAC TGGGAAGCCT GAATCTGCGG TGGCAGGAGG TCTGCAAACA GCTGGCAGAA 
AGAAAAAAGA GGCTAGAGGA ACAGAAGAAT ATCTTGTCAG AATTTCAAAG AGATGTAAAT 
GAATTTGTTT TATGGTTGGA AGAAGCGGAT AACGTTGCTA ATATTCCACT TGAACCTGGA 
AATGAGCAGC AGCTAAAAGA AAAACTTGAA CAAGTCAAGT TACTGGCAGA AGAGTTGCCC 
CTGCGCCAGG GAATTCTAAA ACAATTAAAT GAAACTGGAG GAACAGTGCT TGTAAGTGCT 
CCCCTAAGCC CAGAAGAGCA AGATAAACTT GAAAATAAGC TCAAGCAGAC AAATCTTCAG 
TGGATAAAGG TTTCTAGAAA TCTGCCTGAG AAGCAAGAAG AAATTGAGGC ACACGTAAAA 
GACCTTGGAC AGCTGGAAGA GCAGTTAAAT CATCTGCTTC TATGGCTGTC TCCTATTAGG 
AATCAGTTGG AAATTTACAA TCAGCCAAAT CAAACAGGAC CATTTGACAT CAAGGAAATT 
GAAGTAGCAG TTCAAGCTAA ACAGCCGGAT GTGGAAGGGA TTTTGTCTAA AGGGCAGCAT 
TTGTACAAGG AAAAACCAGC CACTCAGCCA GCGAAGAGAA AGCTGGAAGA TCTCAGCTCT 
GATTGGAAGG TGGTAACTCA GTTGCTTCAA GAGCTGCGGG CAAAGCAGCC TGGCCCAGCT 
CCTGGACTGA CCACTGTCAG AGCCCCTCCC AGTCAGACTG TTACTCTGGT GACACAACCC 
GCGGTTACCA AGGAAACTGC CATCTCCAAA CTAGAAATGC CATCTTCATT GCTGTTGGAG 
GTACCTGCAC TGGCAGATTT CAACCGAGCT TGGACAGAAC TTACCGACTG GCTGTCTCTG 
CTTGATCGAG TTATAAAATC ACAGAGGGTG ATGGTGGGTG ATCTTGAAGA CATTAACGAG 
ATGATCATCA AGCAGAAGGC AACGCTGCAG GATTTGGAAC AGAGGCGCCC CCAGTTGGAA 
GAACTCATTA CCGCTGCCCA GAATTTGAAA AACAAGACCA GCAATCAAGA GGCTAGAACA 
ATCATTACTG ATCGAATTGA AAGAATTCAG AGTCAGTGGG ATGAAGTACA GGAACATCTT 
CAGAACCGGA GGCAACAGTT GAATGAAATG TTAAAGGATT CCACACAATG GCTGGAAGCT 
AAAGAGGAGG CTGAGCAGGT GTTGGGGCAG GCCAGAGCCA AGCTTGAGTC ATGGAAGGAG 
GCTCCCTACA CAGTAGATGC AATCCAAAAG AAAATCACAG AAACCAAGCA GTTGGCCAAA 
GACCTCCGCC AGTGGCAGAT AAATGTAGAT GTTGCAAATG ATTTGGCACT GAAACTTCTC 
CGAGATTATT CTGCAGATGA TACCAGAAAA GTACACATGA TAACAGAGAA CATCAATGCC 
TCTTGGGCAA GCATCCATAA AAGATTGAGT GAGCGAGAGG CTGCTCTGGA AGAAACCCAC 
AGATTACTGC AACAGTTCCC CTTGGACCTG GAGAAGTTCC TTGCCTGGCT TACAGAAGCC 
GAAACAACTG CCAACGTCCT GCAGGATGCC ACCCATAAGG AAAGGCTTCT AGAAGATTCC 
AAGGGAGTAA GAGAGCTGAT GAAACAATGG CAAGATCTCC AAGGAGAAAT CGAAGCTCAC 
ACAGATATCT ATCACAACCT GGACGAAAAT GGCCAAAAAG TCCTGAGATC CCTGGAAGGT 
TCTGACGATG CAGCCTTGTT GCAAAGACGT TTGGATAACA TGAACTTCAA GTGGAGCGAA 
CTTCGGAAAA AGTCTCTCAA CATTAGGTCT CACTTGGAAG CCAGTTCTGA CCAGTGGAAG 
CGTCTGCACC TTTCTCTTCA GGAACTTCTG GTATGGCTCC AGCTGAAAGA TGATGAGTTA 
AGCCGGCAGG CACCCATTGG AGGAGACTTT CCAGCGGTGC AGAAGCAGAA TGATGTACAC 
AGGGCCTTCA AGAGGGAATT GAAAACGAAA GAACCTGTAA TCATGAGTAC TCTTGAGACT 
GTACGAATAT TTCTGACAGA GCAGCCTTTA GAAGGACTAG AGAAACTCTA CCAGGAGCCC 
AGAGAGCTGC CTCCTGAAGA GAGAGCCCAG AATGTCACAC GGCTCCTACG AAAGCAAGCT 
GAGGAGGTCA ACACTCAGTG GGAAAAACTG AACGTGCACT CTGCAGACTG GCAGAGAAAA 
ATAGACGAGG CCCTCGAAAG ACTCCAGGAG CTTCAGGAAG CAACAGATGA GCTGGATCTC 
AAACTACGTC AGGCAGAGGT GATCAAAGGA TCCTGGCAGC CTGTGGGTGA TCTCCTCATT 
GACTCTCTCC AAGATCACCT CGAAAAAGTC AAGGCGCTTC GAGGAGAAAT TACACCTCTG 
AAAGAGAATG TCAGCTACGT CAATGACCTT GCTCGCCAAC TCACTACGTT GGGCATTCAG 
CTGTCACCAT ATAACCTCAA CACTCTGGAA GACCTGAACA CCAGATGGAA GCTTCTGCAG 
GTGGCCATTG AGGACCGCAT CAGGCAGCTG CATGAAGCGC ACAGGGACTT TGGACCAGCC 
TCCCAGCACT TCCTTTCCAC TTCTGTCCAG GGTCCCTGGG AGAGAGCCAT CTCACCAAAC 
AAAGTGCCCT ACTATATCAA CCACGAGACC CAAACAACTT GCTGGGACCA TCCCAAAATG 
ACAGAGCTCT ACCAGTCTTT AGCTGACCTG AATAATGTCA GGTTCTCAGC TTACAGGACT 
GCCATGAAAC TCCGAAGACT GCAGAAGGCC CTTTGCTTGG ATCTCTTGAG CCTATCGGCT 
GCATGCGATG CCTTGGACCA GCACAACCTC AAGCAAAATG ACCAGCCCAT GGATATCCTG 
CAGGTCATTA ACTGTCTGAC CACTATTTAT GATCGCCTAG AGCAAGAGCA CAACAATCTG 
GTCAACGTCC CTCTCTGCGT GGATATGTGT CTCAATTGGC TGCTGAATGT TTATGACACG 
GGACGAACGG GGAGGATCCG GGTCCTGTCT TTTAAAACTG GCATCATTTC TCTGTGTAAA 
GCCCATTTGG AAGACAAGTA CAGATACCTC TTCAAGCAAG TGGCAAGTTC GACAGGATTT 
TGTGACCAGC GCAGGCTGGG CCTCCTCCTG CATGACTCTA TCCAGATCCC AAGACAGTTG 
GGTGAAGTCG CATCGTTCGG GGGCAGTAAC ATTGAGCCGA GTGTCAGGAG CTGCTTCCAG 
TTTGCTAATA ATAAGCCTGA GATCGAAGCG GCCCTCTTCC TAGACTGGAT GCGCCTGGAG 
CCCCAGTCCA TGGTGTGGCT GCCTGTCCTG CACCGAGTGG CTGCCGCGGA AACTGCCAAG 
CACCAGGCCA AGTGCAACAT CTGCAAGGAG TGTCCCATCA TCGGATTCAG GTACAGGAGT 
CTAAAGCACT TTAATTATGA CATCTGCCAA AGTTGCTTTT TTTCTGGTCG AGTTGCAAAA 
GGCCATAAAA TGCACTATCC CATGGTGGAA TACTGCACTC CGACTACATC GGGAGAAGAT 
GTCCGTGACT TTGCCAAGGT ACTAAAAAAC AAATTTCGAA CCAAAAGGTA TTTTGCGAAG 
CATCCCCGAA TGGGCTACCT GCCAGTGCAG ACTGTCTTAG AGGGGGACAA CATGGAAACT 
CCTGTCACTC TGATCAACTT CTGGCCGGTA GATTCTGCGC CTGCCTCGTC CCCTCAGCTT 
TCACACGATG ATACTCATTC ACGCATTGAG CATTATGCTA GCAGGCTAGC AGAAATGGAA 
AACAGCAATG GATCTTATCT AAATGATAGC ATCTCTCCTA ATGAGAGCAT AGATGATGAA 
CATTTGTTAA TCCAGCATTA CTGCCAAAGT TTGAACCAGG ACTCCCCCCT GAGCCAGCCT 
CGTAGTCCTG CCCAGATCTT GATTTCCTTA GAGAGTGAGG AAAGAGGGGA GCTAGAGAGA 
ATCCTAGCAG ATCTTGAGGA AGAAAACAGA AATCTGCAAG CAGAGTATGA TCGTCTAAAG 
CAGCAGCATG AACACAAAGG CCTGTCCCCA CTGCCATCCC CTCCTGAAAT GATGCCTACT 
TCTCCCCAAA GTCCCCGGGA TGCTGAGCTC ATCGCTGAGG CCAAGCTGCT GCGTCAACAC 
AAAGGCCGCC TGGAAGCCAG GATGCAAATC CTTGAAGACC ATAACAAACA ACTGGAATCC 
CAGTTACACA GGCTCAGGCA GCTGCTGGAG CAACCCCAGG CAGAGGCCAA GGTGAATGGT 
ACAACGGTGT CTTCTCCTTC TACCTCTCTT CAGAGGTCAG ATAGCAGTCA GCCTATGCTG 
CTCCGGGTGG TCGGCAGTCA GACTTCAGAA TCCATGGGCG AGGAAGACCT GCTCAGCCCT 
CCCCAGGACA CAAGCACAGG GTTAGAGGAA GTGATGGAGC AGCTCAACCA CTCCTTCCCT 
AGTTCCAGAG GACACAATGT AGGAAGCCTT TTCCACTTGG CAGATGATTT GGGCAGAGCG 
ATGGAGTCCT TAGTTTCAGT CATGACAGAT GAAGAAGGGG CAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMD ( XM_005641029.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005641086.1 Canis lupus familiaris dystrophin (DMD), transcript variant X1, mRNA.

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CloneID	OCe31973
Clone ID Related Accession (Same CDS sequence)	XM_005641029.3 , XM_005641029.2
Accession Version	XM_005641029.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	dystrophin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005641029.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTGGT GGGAAGAAGT AGAGGACTGT TATGAAAGAG AAGATGTTCA AAAGAAAACA 
TTCACAAAAT GGATAAATGC ACAGTTTTCT AAGTTTGGGA AGCAGCACAT AGAGAACCTC 
TTCAGTGACC TACAGGATGG GAGACGCCTC CTAGACCTTT TGGAAGGCCT GACAGGGCAA 
AAACTGCCAA AAGAAAAAGG ATCCACAAGA GTTCATGCCC TGAACAATGT CAACAAGGCA 
CTGCGCGTCT TGCAGAAAAA TAATGTTGAT TTAGTGAACA TTGGAAGTAC TGACATAGTA 
GATGGAAATC ACAAACTGAC TCTTGGTTTG ATTTGGAATA TAATCCTCCA CTGGCAGGTC 
AAAAATGTAA TGAAAAATAT CATGGCTGGA TTGCAACAAA CCAACAGTGA AAAGATTCTC 
CTGAGCTGGG TCCGACAATC AACTCGTAAT TATCCACAGG TTAATGTCAT TAACTTCACC 
ACCAGCTGGT CTGATGGCCT GGCTTTGAAC GCTCTCATCC ACAGTCATAG GCCAGACCTG 
TTTGATTGGA ATAGTGTGGT TTGCCAGCAG TCAGCCACAC AACGCCTGGA ACATGCATTC 
AACATTGCCA AATATCAATT AGGCATAGAG AAACTGCTTG ATCCTGAAGA TGTTGCCACC 
ACTTATCCAG ATAAGAAGTC CATCTTAATG TATATCACAT CACTCTTCCA AGTTTTGCCT 
CAACAAGTGA GCATTGAAGC CATCCAGGAA GTGGAAATGT TGCCAAGGCC ATCAAAAGTT 
ACTAGAGAAG AACATTTTCA ATTACATCAT CAAATGCACT ATTCTCAACA GATCACAGTC 
AGTCTAGCAC AGGGCTATGA ACGAGCCCCT TCCTCTCCTA AGCCTCGGTT CAAGAGCTAT 
GCCTACACAC AGGCTGCTTA TGTCACCACT TCTGACCCCA CACGGAGCCC ACTTCCTTCA 
CAGCATTTGG AAACTCCTGA AGACAAGTCA TTTGGCCGGT CATTGACAGA GACCGAAGCA 
AACCTGGACA GTTATCAAAC AGCTTTGGAA GAAGTACTCT CGTGGCTTCT TTCAGCTGAG 
GATGCACTGC AAGCCCAAGG AGAGATTTCT AATGATGTCG AAGAAGTGAA AGAACAATTT 
CATACTCATG AGGGATATAT GATGGACTTG ACATCCCATC AGGGACGGGT CGGTAATGTT 
CTCCAACTGG GAAGTCAACT GATTGGAACA GGGAAATTAT CAGAAGATGA AGAAACCGAA 
GTGCAGGAAC AAATGAATCT CCTCAATTCA AGATGGGAAT GCCTCAGGGT AGCTAGCATG 
GAAAAACAAA GCAATTTACA TAAAGTTCTA ATGGATCTCC AGAATCAGCA ACTGAAAGAG 
TTAAATGACT GGCTAACCAA AACAGAAGAG AGAACAAGGA AAATGGAGAA GGAGCCCCTT 
GGACCTGATA TTGAAGACCT AAAACGCCAA GTACAACAAC ATAAGGTGCT TCAAGAAGAC 
TTAGAACAGG AACAAGTCAG GGTCAATTCC CTCACTCATA TGGTGGTGGT AGTCGATGAA 
TCTAGTGGAG ACCATGCAAC TGCTGCTTTG GAAGAACAAC TTAAGGTACT GGGAGATCGA 
TGGGCAAACA TCTGTAGGTG GACAGAAGAT CGCTGGGTTC TTTTACAAGA CATCCTCCTA 
AAATGGCAGC GTTTTACTGA AGAACAGTGC CTTTTCAGTG CATGGCTTTC GGAGAAGGAA 
GATGCAGTGA ACAAGATTCA CACAACTGGC TTTAAGGATC AAAGTGAAGT GTTATCAAAT 
CTTCAGAAAC TGGCTGTCTT AAAAACAGAT CTGGAAAAGA AGAAGCAAAC CATGGACAAA 
CTCTGCTCAC TCAACCAAGA CCTTCTTTCA GCGCTGAAAA ACACAGTGGT AGCCCACAAG 
ATGGAAGCAT GGCTGGACAA CTTTGCCCAG CGCTGGGATA ATTTAGTCCA GAAACTTGAA 
AAAAGTTCAG CACAGATTTC ACAGGCTGTC ACCACCACTC AGCCATCACT AACACAGACA 
ACTGTAATGG AAACAGTAAC TATGGTGACC ACGAGGGAAC ACATCTTGGT AAAGCATGCC 
CAAGAGGAAC TGCCACCACC ACCCCCTCAG AAGAAGAGGC AGATTATCGT GGATTCTGAA 
ATTAGGAAAA GGTTGGATGT CGATATAACT GAACTTCACA GTTGGATTAC TCGTTCAGAA 
GCTGTGTTGC AGAGTCCTGA ATTTGCAATC TATCGGAAGG AAGGCAACTT CTCAGACCTT 
AAAGAAAAAG TCAATGCCAT AGAGCGAGAA AAAGCCGAGA AGTTCAGAAA ACTGCAAGAT 
GCCAGCAGAT CAGCTCAGGC CCTGGTGGAA CAGATGGTGA ATGAGGGTGT TAATGCTGAC 
AGCATCAAAC AAGCCTCCGA ACAACTGAAC AGCCGGTGGA TAGAGTTCTG CCAATTGCTA 
AGCGAGAGAC TTAACTGGCT GGAGTATCAG AACAACATCA TCACTTTCTA TAATCAGCTA 
CAACAATTGG AGCAGATGAC AACTACTGCT GAAAACTGGT TGAAAACCCA GCCTACCACC 
ACATCAGAGC CAACAGCAAT TAAAAGCCAG TTAAAAATTT GTAAGGATGA AATCAACCGA 
CTGTCAGCTC TTCAGCCTCA AATCGAGCGA TTAAAAATTC AAAGCATAGC CCTGAAAGAG 
AAAGGACAAG GGCCAATGTT CCTGGATGCA GACTTTGTGG CCTTTACAAA TCATTTTAAC 
CAAGTCTTTG CTGATGTGCA GGCAAGAGAA AAAGAGCTAC AAACAATTTT TGACAGTTTG 
CCACCCATGC GCTATCAGGA GACTATGAGT ACCATCCTGA CATGGATCCA GCAGTCAGAA 
ACCAAACTCT CTATACCTCA GGTTACTGTC ACTGAATATG ACATCATGGA ACAGAGACTC 
GGAGAGCTAC AGGCTTTACA AAGCTCTCTG CAAGAGCAAC AAAATGGCCT AAACTATCTC 
AGCACCACTG TGAAAGAGAT GTCAAAGAAA GCACCACTGT CTGATATTAG TCGGAAATAT 
CAATCAGAAT TTGAAGAGAT TGAGGGACGT TGGAAGAAGC TGTCTTCCCA GCTGGTTGAA 
CATTGTCAAA AGTTGGAGGA GCAAATGGCT AAACTTCGAA AAATTCAGAA TCACATAAAA 
ACTCTGAAGA AATGGATCAC TGAAGTCGAT GTTTTCCTGA AGGAGGAATG GCCTGCCCTT 
GGGGATTCAG AAATTCTGAA AAGACAGCTG AAACAGTGCA GGCTTTTAGT CAATGACATT 
CAGACCATCC AGCCTAGTCT CAACAGTGTC AATGAAGGGG CTCAGAAGAT GAAGAATGAA 
GCAGAACCAG AGTTTGCTGG CAGACTTGAG ACAGAGCTCC GAGAACTTAA CACCCAGTGG 
GATTACATGT GCCGCCAGGT CTATGCCAGG AAGGAAGCCT TAAAAGGAGG TTTGGATAAA 
ACTGTAAGTC TTCAGAAAGA TCTGTCAGAG ATGCATGAGT GGATGACACA AGCTGAAGAA 
GAATACCTAG AGAGAGATTT CGAATACAAG ACCCCTGATG AATTACAGAC AGCAGTTGAA 
GAGATGAAGA GAGCTAAAGA AGAGGCCCAG CAAAAAGAAG CAAAAGTGAA ACTCCTAACC 
GAGTCCGTCA ATAGTGTCAT AGCTCAGGCT CCACCTGCAG CACAAGAGGC CTTAAAAAAG 
GAACTTGACA CTCTCACCAC CAACTACCAG TGGCTCTGCA CCAGGCTCAA TGGCAAATGC 
AAGACCTTGG AAGAAGTTTG GGCGTGCTGG CATGAGTTAT TGTCCTACTT GGAGAAGGCA 
AACAAGTGGC TAAGTGAAGT AGAAGTCAAG CTTAAAACCA CTGAAAATAT TTCTGGGGGA 
GCTGAGGAAA TCGCCGAGGT GCTTGATTCG CTTGAAAATT TGATGCAACA TTCAGAGGAT 
AACCCGAATC AGATTCGCAT ATTGGCACAG ACCTTGACAG ATGGTGGAGT CATGGATGAA 
CTGATCAATG AGGAGCTTGA GACATTTAAT TCTCGTTGGA GAGAACTCCA TGAAGAGGCT 
GTGAGGAGGC AAAAGTTGCT TGAGCAGAGT ATCCAGTCGG CCCAGGAGAT AGAAAAATCC 
TTGCACTTAA TTCAGGAGTC CCTCTCTTCC ATTGACAAGC AGTTGGCAGC TTATATTGCT 
GACAAAGTGG ATGCAGCTCA GATGCCTCAG GAAGCCCAGA AAATCCAATC AGATTTGACA 
AGTCATGAGA TCAGTTTAGA AGAAATGAAG AAACATAACC AGGGAAAGGA GACTGCCCAA 
AGGGTACTAT CCCAAATTGA TGTGGCACAG AAAAAATTGC AGGATGTTTC CATGAAGTTT 
CGATTATTCC AGAAGCCAGC CAATTTTGAG CAGCGCCTAC AAGAAAGTAA AATGATTTTA 
GATGAAGTGA AGATGCATTT ACCTGCGTTG GAAACAAAGA GTGTGGAACA GGAAGTAGTA 
CAGTCACAGT TAAATCATTG TGTGAACTTG TATAAAAGTC TGAGTGAAGT GAAGTCTGAA 
GTGGAAATGG TAATAAAAAC TGGACGTCAG ATTGTACAGA AGAAGCAGAC GGAAAACCCG 
AAAGAGCTTG ATGAAAGAGT TACAGCTTTG AAATTGCATT ATAATGAGCT GGGAGCAAAG 
GTGACAGAAA GAAAGCAACA GTTGGAAAAA TGCTTGAAAT TGTCCCGTAA GATGCGAAAG 
GAAATGAATG CCCTGACAGA ATGGCTGGCA GCGACAGATA TGGAACTGAC GAAGAGATCG 
GCAGTTGAAG GAATGCCTAG TAATTTGGAT TCTGAAGTTG CCTGGGGAAA GGCTACTCAG 
AAAGAGATTG AGAAACAGAA GGTTCACCTA AAGAGTGTCA CAGAGGTAGG AGAGGCCTTG 
AAAACGGTTT TGGGCAAGAA GGAAATGTTG GTGGAAGATA AACTGAGTCT TCTGAATAGT 
AACTGGATAG CCGTCACTTC CCGAGCAGAA GAGTGGTTAA ACCTTTTATT GGAATACCAG 
AAACACATGG AAAACTTTGA CCAGAATGTG GATTACATCA CAAACTGGAT CATTCAGGCT 
GATGCACTTT TGGATGAATC TGAGAAAAAG AAACCTCAGC AAAAAGAAGA CATACTTAAG 
CGTTTAAAGG CTGAAATGAA TGACATACGT CCAAAGGTGG ATTCTACACG TGACCAAGCA 
GCAAACCTGA TGGCAAACCG CGGCGACCAC TGCAGGAAAG TAGTAGAGCC CAAAATCTCA 
GAGCTCAACC ATCGATTTGC AGCCATTTCT CACAGAATTA AGACTGGAAA GGCCTCCATT 
CCTTTGAAGG AATTGGAGCA GTTTAACTCA GATATACAAA AATTGCTTGA ACCACTGGAG 
GCTGAAATTC AGCAGGGGGT GAATCTGAAA GAGGAAGACT TCAATAAAGA TATGAGTGAA 
GACAATGAGG GTACTGTAAA AGAATTGTTG CAAAGAGGAG ACAACTTACA ACAAAGAATC 
ACAGATGAGA GAAAGCGAGA GGAAATAAAG ATAAAACAAC AGCTGTTACA GACAAAACAT 
AATGCTCTCA AGGATTTGAG GTCTCAAAGA AGAAAAAAGG CTCTAGAAAT TTCTCACCAG 
TGGTATCAGT ACAAGAGGCA GGCTGATGAT CTCCTGAAAT GCTTGGATGA CATTGAAAAA 
AAATTAGCCA GCCTACCTGA ACCCAGAGAT GAAAGGAAAA TAAAGGAAAT TGATCGTGAA 
TTGCAGAAGA AGAAAGAGGA GCTGAATGCA GTGCGTAGGC AAGCTGAGGG CTTGTCTGAG 
GATGGGGCCG CAATGGCAGT GGAGCCAACT CAGATCCAGC TCAGCAAGCG CTGGCGGGAA 
ATTGAGAGCA AATTTGCTCA GTTTCGAAGA CTCAACTTTG CACAAATTCA CACTGTCCAT 
GAAGAGTCAG TGGTGGCGAT GACTGAAGAC ATGCCTTTGG AAATTTCTTA TGTGCCTTCT 
ACTTACCTGA CTGAGATCAC TCATGTCTCA CAAGCCCTAT CAGAAGTGGA AGAGCTTCTT 
AATGCTCCCG ACCTTTGTGC TCAAGATTTT GAAGATCTCT TTAAACAAGA GGAATCCTTG 
AAGAACATAA AAGACAGCCT GCAACAAATC TCAGGTCGGA TTGACATCAT TCACAATAAA 
AAGACAGCAG CATTGCACAG TGCCACTCCT GCAGAAAGGG CAAAGCTCCA GGAAGCTCTC 
TCACGGCTTG ATTTCCAATG GGAAAGAGTT AACAATATGT ACAAGGACCG ACAAGGGAGA 
TTTGACAGAT CTGTGGAAAA ATGGCGGCGG TTTCATTATG ATATGAAGAT ACTTAATCAA 
TGGCTAACAG AAGCTGAACA GTTTCTCAAA AAGACACAAA TTCCTGAGAA TTGGGAACAT 
GCCAAATACA AATGGTATCT TAAGGAACTC CAGGATGGCA TTGGACAGCG GCAAAGTGTT 
GTCAGGGTAT TGAATGCAAC TGGGGAAGAA ATAATTCAAC AGTCCTCAAA AACAGATGCC 
AGTATTCTCC AAGAAAAACT GGGAAGCCTG AATCTGCGGT GGCAGGAGGT CTGCAAACAG 
CTGGCAGAAA GAAAAAAGAG GCTAGAGGAA CAGAAGAATA TCTTGTCAGA ATTTCAAAGA 
GATGTAAATG AATTTGTTTT ATGGTTGGAA GAAGCGGATA ACGTTGCTAA TATTCCACTT 
GAACCTGGAA ATGAGCAGCA GCTAAAAGAA AAACTTGAAC AAGTCAAGTT ACTGGCAGAA 
GAGTTGCCCC TGCGCCAGGG AATTCTAAAA CAATTAAATG AAACTGGAGG AACAGTGCTT 
GTAAGTGCTC CCCTAAGCCC AGAAGAGCAA GATAAACTTG AAAATAAGCT CAAGCAGACA 
AATCTTCAGT GGATAAAGGT TTCTAGAAAT CTGCCTGAGA AGCAAGAAGA AATTGAGGCA 
CACGTAAAAG ACCTTGGACA GCTGGAAGAG CAGTTAAATC ATCTGCTTCT ATGGCTGTCT 
CCTATTAGGA ATCAGTTGGA AATTTACAAT CAGCCAAATC AAACAGGACC ATTTGACATC 
AAGGAAATTG AAGTAGCAGT TCAAGCTAAA CAGCCGGATG TGGAAGGGAT TTTGTCTAAA 
GGGCAGCATT TGTACAAGGA AAAACCAGCC ACTCAGCCAG CGAAGAGAAA GCTGGAAGAT 
CTCAGCTCTG ATTGGAAGGT GGTAACTCAG TTGCTTCAAG AGCTGCGGGC AAAGCAGCCT 
GGCCCAGCTC CTGGACTGAC CACTGTCAGA GCCCCTCCCA GTCAGACTGT TACTCTGGTG 
ACACAACCCG CGGTTACCAA GGAAACTGCC ATCTCCAAAC TAGAAATGCC ATCTTCATTG 
CTGTTGGAGG TACCTGCACT GGCAGATTTC AACCGAGCTT GGACAGAACT TACCGACTGG 
CTGTCTCTGC TTGATCGAGT TATAAAATCA CAGAGGGTGA TGGTGGGTGA TCTTGAAGAC 
ATTAACGAGA TGATCATCAA GCAGAAGGCA ACGCTGCAGG ATTTGGAACA GAGGCGCCCC 
CAGTTGGAAG AACTCATTAC CGCTGCCCAG AATTTGAAAA ACAAGACCAG CAATCAAGAG 
GCTAGAACAA TCATTACTGA TCGAATTGAA AGAATTCAGA GTCAGTGGGA TGAAGTACAG 
GAACATCTTC AGAACCGGAG GCAACAGTTG AATGAAATGT TAAAGGATTC CACACAATGG 
CTGGAAGCTA AAGAGGAGGC TGAGCAGGTG TTGGGGCAGG CCAGAGCCAA GCTTGAGTCA 
TGGAAGGAGG CTCCCTACAC AGTAGATGCA ATCCAAAAGA AAATCACAGA AACCAAGCAG 
TTGGCCAAAG ACCTCCGCCA GTGGCAGATA AATGTAGATG TTGCAAATGA TTTGGCACTG 
AAACTTCTCC GAGATTATTC TGCAGATGAT ACCAGAAAAG TACACATGAT AACAGAGAAC 
ATCAATGCCT CTTGGGCAAG CATCCATAAA AGATTGAGTG AGCGAGAGGC TGCTCTGGAA 
GAAACCCACA GATTACTGCA ACAGTTCCCC TTGGACCTGG AGAAGTTCCT TGCCTGGCTT 
ACAGAAGCCG AAACAACTGC CAACGTCCTG CAGGATGCCA CCCATAAGGA AAGGCTTCTA 
GAAGATTCCA AGGGAGTAAG AGAGCTGATG AAACAATGGC AAGATCTCCA AGGAGAAATC 
GAAGCTCACA CAGATATCTA TCACAACCTG GACGAAAATG GCCAAAAAGT CCTGAGATCC 
CTGGAAGGTT CTGACGATGC AGCCTTGTTG CAAAGACGTT TGGATAACAT GAACTTCAAG 
TGGAGCGAAC TTCGGAAAAA GTCTCTCAAC ATTAGGTCTC ACTTGGAAGC CAGTTCTGAC 
CAGTGGAAGC GTCTGCACCT TTCTCTTCAG GAACTTCTGG TATGGCTCCA GCTGAAAGAT 
GATGAGTTAA GCCGGCAGGC ACCCATTGGA GGAGACTTTC CAGCGGTGCA GAAGCAGAAT 
GATGTACACA GGGCCTTCAA GAGGGAATTG AAAACGAAAG AACCTGTAAT CATGAGTACT 
CTTGAGACTG TACGAATATT TCTGACAGAG CAGCCTTTAG AAGGACTAGA GAAACTCTAC 
CAGGAGCCCA GAGAGCTGCC TCCTGAAGAG AGAGCCCAGA ATGTCACACG GCTCCTACGA 
AAGCAAGCTG AGGAGGTCAA CACTCAGTGG GAAAAACTGA ACGTGCACTC TGCAGACTGG 
CAGAGAAAAA TAGACGAGGC CCTCGAAAGA CTCCAGGAGC TTCAGGAAGC AACAGATGAG 
CTGGATCTCA AACTACGTCA GGCAGAGGTG ATCAAAGGAT CCTGGCAGCC TGTGGGTGAT 
CTCCTCATTG ACTCTCTCCA AGATCACCTC GAAAAAGTCA AGGCGCTTCG AGGAGAAATT 
ACACCTCTGA AAGAGAATGT CAGCTACGTC AATGACCTTG CTCGCCAACT CACTACGTTG 
GGCATTCAGC TGTCACCATA TAACCTCAAC ACTCTGGAAG ACCTGAACAC CAGATGGAAG 
CTTCTGCAGG TGGCCATTGA GGACCGCATC AGGCAGCTGC ATGAAGCGCA CAGGGACTTT 
GGACCAGCCT CCCAGCACTT CCTTTCCACT TCTGTCCAGG GTCCCTGGGA GAGAGCCATC 
TCACCAAACA AAGTGCCCTA CTATATCAAC CACGAGACCC AAACAACTTG CTGGGACCAT 
CCCAAAATGA CAGAGCTCTA CCAGTCTTTA GCTGACCTGA ATAATGTCAG GTTCTCAGCT 
TACAGGACTG CCATGAAACT CCGAAGACTG CAGAAGGCCC TTTGCTTGGA TCTCTTGAGC 
CTATCGGCTG CATGCGATGC CTTGGACCAG CACAACCTCA AGCAAAATGA CCAGCCCATG 
GATATCCTGC AGGTCATTAA CTGTCTGACC ACTATTTATG ATCGCCTAGA GCAAGAGCAC 
AACAATCTGG TCAACGTCCC TCTCTGCGTG GATATGTGTC TCAATTGGCT GCTGAATGTT 
TATGACACGG GACGAACGGG GAGGATCCGG GTCCTGTCTT TTAAAACTGG CATCATTTCT 
CTGTGTAAAG CCCATTTGGA AGACAAGTAC AGATACCTCT TCAAGCAAGT GGCAAGTTCG 
ACAGGATTTT GTGACCAGCG CAGGCTGGGC CTCCTCCTGC ATGACTCTAT CCAGATCCCA 
AGACAGTTGG GTGAAGTCGC ATCGTTCGGG GGCAGTAACA TTGAGCCGAG TGTCAGGAGC 
TGCTTCCAGT TTGCTAATAA TAAGCCTGAG ATCGAAGCGG CCCTCTTCCT AGACTGGATG 
CGCCTGGAGC CCCAGTCCAT GGTGTGGCTG CCTGTCCTGC ACCGAGTGGC TGCCGCGGAA 
ACTGCCAAGC ACCAGGCCAA GTGCAACATC TGCAAGGAGT GTCCCATCAT CGGATTCAGG 
TACAGGAGTC TAAAGCACTT TAATTATGAC ATCTGCCAAA GTTGCTTTTT TTCTGGTCGA 
GTTGCAAAAG GCCATAAAAT GCACTATCCC ATGGTGGAAT ACTGCACTCC GACTACATCG 
GGAGAAGATG TCCGTGACTT TGCCAAGGTA CTAAAAAACA AATTTCGAAC CAAAAGGTAT 
TTTGCGAAGC ATCCCCGAAT GGGCTACCTG CCAGTGCAGA CTGTCTTAGA GGGGGACAAC 
ATGGAAACTC CTGTCACTCT GATCAACTTC TGGCCGGTAG ATTCTGCGCC TGCCTCGTCC 
CCTCAGCTTT CACACGATGA TACTCATTCA CGCATTGAGC ATTATGCTAG CAGGCTAGCA 
GAAATGGAAA ACAGCAATGG ATCTTATCTA AATGATAGCA TCTCTCCTAA TGAGAGCATA 
GATGATGAAC ATTTGTTAAT CCAGCATTAC TGCCAAAGTT TGAACCAGGA CTCCCCCCTG 
AGCCAGCCTC GTAGTCCTGC CCAGATCTTG ATTTCCTTAG AGAGTGAGGA AAGAGGGGAG 
CTAGAGAGAA TCCTAGCAGA TCTTGAGGAA GAAAACAGAA ATCTGCAAGC AGAGTATGAT 
CGTCTAAAGC AGCAGCATGA ACACAAAGGC CTGTCCCCAC TGCCATCCCC TCCTGAAATG 
ATGCCTACTT CTCCCCAAAG TCCCCGGGAT GCTGAGCTCA TCGCTGAGGC CAAGCTGCTG 
CGTCAACACA AAGGCCGCCT GGAAGCCAGG ATGCAAATCC TTGAAGACCA TAACAAACAA 
CTGGAATCCC AGTTACACAG GCTCAGGCAG CTGCTGGAGC AACCCCAGGC AGAGGCCAAG 
GTGAATGGTA CAACGGTGTC TTCTCCTTCT ACCTCTCTTC AGAGGTCAGA TAGCAGTCAG 
CCTATGCTGC TCCGGGTGGT CGGCAGTCAG ACTTCAGAAT CCATGGGCGA GGAAGACCTG 
CTCAGCCCTC CCCAGGACAC AAGCACAGGG TTAGAGGAAG TGATGGAGCA GCTCAACCAC 
TCCTTCCCTA GTTCCAGAGG ACACAATGTA GGAAGCCTTT TCCACTTGGC AGATGATTTG 
GGCAGAGCGA TGGAGTCCTT AGTTTCAGTC ATGACAGATG AAGAAGGGGC AGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005641086.1
CDS	247..11343
Translation	MLWWEEVEDCYEREDVQKKTFTKWINAQFSKFGKQHIENLFSDLQDGRRLLDLLEGLTGQKLPKEKGSTRVHALNNVNKALRVLQKNNVDLVNIGSTDIVDGNHKLTLGLIWNIILHWQVKNVMKNIMAGLQQTNSEKILLSWVRQSTRNYPQVNVINFTTSWSDGLALNALIHSHRPDLFDWNSVVCQQSATQRLEHAFNIAKYQLGIEKLLDPEDVATTYPDKKSILMYITSLFQVLPQQVSIEAIQEVEMLPRPSKVTREEHFQLHHQMHYSQQITVSLAQGYERAPSSPKPRFKSYAYTQAAYVTTSDPTRSPLPSQHLETPEDKSFGRSLTETEANLDSYQTALEEVLSWLLSAEDALQAQGEISNDVEEVKEQFHTHEGYMMDLTSHQGRVGNVLQLGSQLIGTGKLSEDEETEVQEQMNLLNSRWECLRVASMEKQSNLHKVLMDLQNQQLKELNDWLTKTEERTRKMEKEPLGPDIEDLKRQVQQHKVLQEDLEQEQVRVNSLTHMVVVVDESSGDHATAALEEQLKVLGDRWANICRWTEDRWVLLQDILLKWQRFTEEQCLFSAWLSEKEDAVNKIHTTGFKDQSEVLSNLQKLAVLKTDLEKKKQTMDKLCSLNQDLLSALKNTVVAHKMEAWLDNFAQRWDNLVQKLEKSSAQISQAVTTTQPSLTQTTVMETVTMVTTREHILVKHAQEELPPPPPQKKRQIIVDSEIRKRLDVDITELHSWITRSEAVLQSPEFAIYRKEGNFSDLKEKVNAIEREKAEKFRKLQDASRSAQALVEQMVNEGVNADSIKQASEQLNSRWIEFCQLLSERLNWLEYQNNIITFYNQLQQLEQMTTTAENWLKTQPTTTSEPTAIKSQLKICKDEINRLSALQPQIERLKIQSIALKEKGQGPMFLDADFVAFTNHFNQVFADVQAREKELQTIFDSLPPMRYQETMSTILTWIQQSETKLSIPQVTVTEYDIMEQRLGELQALQSSLQEQQNGLNYLSTTVKEMSKKAPLSDISRKYQSEFEEIEGRWKKLSSQLVEHCQKLEEQMAKLRKIQNHIKTLKKWITEVDVFLKEEWPALGDSEILKRQLKQCRLLVNDIQTIQPSLNSVNEGAQKMKNEAEPEFAGRLETELRELNTQWDYMCRQVYARKEALKGGLDKTVSLQKDLSEMHEWMTQAEEEYLERDFEYKTPDELQTAVEEMKRAKEEAQQKEAKVKLLTESVNSVIAQAPPAAQEALKKELDTLTTNYQWLCTRLNGKCKTLEEVWACWHELLSYLEKANKWLSEVEVKLKTTENISGGAEEIAEVLDSLENLMQHSEDNPNQIRILAQTLTDGGVMDELINEELETFNSRWRELHEEAVRRQKLLEQSIQSAQEIEKSLHLIQESLSSIDKQLAAYIADKVDAAQMPQEAQKIQSDLTSHEISLEEMKKHNQGKETAQRVLSQIDVAQKKLQDVSMKFRLFQKPANFEQRLQESKMILDEVKMHLPALETKSVEQEVVQSQLNHCVNLYKSLSEVKSEVEMVIKTGRQIVQKKQTENPKELDERVTALKLHYNELGAKVTERKQQLEKCLKLSRKMRKEMNALTEWLAATDMELTKRSAVEGMPSNLDSEVAWGKATQKEIEKQKVHLKSVTEVGEALKTVLGKKEMLVEDKLSLLNSNWIAVTSRAEEWLNLLLEYQKHMENFDQNVDYITNWIIQADALLDESEKKKPQQKEDILKRLKAEMNDIRPKVDSTRDQAANLMANRGDHCRKVVEPKISELNHRFAAISHRIKTGKASIPLKELEQFNSDIQKLLEPLEAEIQQGVNLKEEDFNKDMSEDNEGTVKELLQRGDNLQQRITDERKREEIKIKQQLLQTKHNALKDLRSQRRKKALEISHQWYQYKRQADDLLKCLDDIEKKLASLPEPRDERKIKEIDRELQKKKEELNAVRRQAEGLSEDGAAMAVEPTQIQLSKRWREIESKFAQFRRLNFAQIHTVHEESVVAMTEDMPLEISYVPSTYLTEITHVSQALSEVEELLNAPDLCAQDFEDLFKQEESLKNIKDSLQQISGRIDIIHNKKTAALHSATPAERAKLQEALSRLDFQWERVNNMYKDRQGRFDRSVEKWRRFHYDMKILNQWLTEAEQFLKKTQIPENWEHAKYKWYLKELQDGIGQRQSVVRVLNATGEEIIQQSSKTDASILQEKLGSLNLRWQEVCKQLAERKKRLEEQKNILSEFQRDVNEFVLWLEEADNVANIPLEPGNEQQLKEKLEQVKLLAEELPLRQGILKQLNETGGTVLVSAPLSPEEQDKLENKLKQTNLQWIKVSRNLPEKQEEIEAHVKDLGQLEEQLNHLLLWLSPIRNQLEIYNQPNQTGPFDIKEIEVAVQAKQPDVEGILSKGQHLYKEKPATQPAKRKLEDLSSDWKVVTQLLQELRAKQPGPAPGLTTVRAPPSQTVTLVTQPAVTKETAISKLEMPSSLLLEVPALADFNRAWTELTDWLSLLDRVIKSQRVMVGDLEDINEMIIKQKATLQDLEQRRPQLEELITAAQNLKNKTSNQEARTIITDRIERIQSQWDEVQEHLQNRRQQLNEMLKDSTQWLEAKEEAEQVLGQARAKLESWKEAPYTVDAIQKKITETKQLAKDLRQWQINVDVANDLALKLLRDYSADDTRKVHMITENINASWASIHKRLSEREAALEETHRLLQQFPLDLEKFLAWLTEAETTANVLQDATHKERLLEDSKGVRELMKQWQDLQGEIEAHTDIYHNLDENGQKVLRSLEGSDDAALLQRRLDNMNFKWSELRKKSLNIRSHLEASSDQWKRLHLSLQELLVWLQLKDDELSRQAPIGGDFPAVQKQNDVHRAFKRELKTKEPVIMSTLETVRIFLTEQPLEGLEKLYQEPRELPPEERAQNVTRLLRKQAEEVNTQWEKLNVHSADWQRKIDEALERLQELQEATDELDLKLRQAEVIKGSWQPVGDLLIDSLQDHLEKVKALRGEITPLKENVSYVNDLARQLTTLGIQLSPYNLNTLEDLNTRWKLLQVAIEDRIRQLHEAHRDFGPASQHFLSTSVQGPWERAISPNKVPYYINHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQVINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPVTLINFWPVDSAPASSPQLSHDDTHSRIEHYASRLAEMENSNGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKQQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDTSTGLEEVMEQLNHSFPSSRGHNVGSLFHLADDLGRAMESLVSVMTDEEGAE

Target ORF information:

RefSeq Version	XM_005641029.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris dystrophin (DMD), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005641029.3

ORF Insert Sequence:

ATGCTTTGGT GGGAAGAAGT AGAGGACTGT TATGAAAGAG AAGATGTTCA AAAGAAAACA 
TTCACAAAAT GGATAAATGC ACAGTTTTCT AAGTTTGGGA AGCAGCACAT AGAGAACCTC 
TTCAGTGACC TACAGGATGG GAGACGCCTC CTAGACCTTT TGGAAGGCCT GACAGGGCAA 
AAACTGCCAA AAGAAAAAGG ATCCACAAGA GTTCATGCCC TGAACAATGT CAACAAGGCA 
CTGCGCGTCT TGCAGAAAAA TAATGTTGAT TTAGTGAACA TTGGAAGTAC TGACATAGTA 
GATGGAAATC ACAAACTGAC TCTTGGTTTG ATTTGGAATA TAATCCTCCA CTGGCAGGTC 
AAAAATGTAA TGAAAAATAT CATGGCTGGA TTGCAACAAA CCAACAGTGA AAAGATTCTC 
CTGAGCTGGG TCCGACAATC AACTCGTAAT TATCCACAGG TTAATGTCAT TAACTTCACC 
ACCAGCTGGT CTGATGGCCT GGCTTTGAAC GCTCTCATCC ACAGTCATAG GCCAGACCTG 
TTTGATTGGA ATAGTGTGGT TTGCCAGCAG TCAGCCACAC AACGCCTGGA ACATGCATTC 
AACATTGCCA AATATCAATT AGGCATAGAG AAACTGCTTG ATCCTGAAGA TGTTGCCACC 
ACTTATCCAG ATAAGAAGTC CATCTTAATG TATATCACAT CACTCTTCCA AGTTTTGCCT 
CAACAAGTGA GCATTGAAGC CATCCAGGAA GTGGAAATGT TGCCAAGGCC ATCAAAAGTT 
ACTAGAGAAG AACATTTTCA ATTACATCAT CAAATGCACT ATTCTCAACA GATCACAGTC 
AGTCTAGCAC AGGGCTATGA ACGAGCCCCT TCCTCTCCTA AGCCTCGGTT CAAGAGCTAT 
GCCTACACAC AGGCTGCTTA TGTCACCACT TCTGACCCCA CACGGAGCCC ACTTCCTTCA 
CAGCATTTGG AAACTCCTGA AGACAAGTCA TTTGGCCGGT CATTGACAGA GACCGAAGCA 
AACCTGGACA GTTATCAAAC AGCTTTGGAA GAAGTACTCT CGTGGCTTCT TTCAGCTGAG 
GATGCACTGC AAGCCCAAGG AGAGATTTCT AATGATGTCG AAGAAGTGAA AGAACAATTT 
CATACTCATG AGGGATATAT GATGGACTTG ACATCCCATC AGGGACGGGT CGGTAATGTT 
CTCCAACTGG GAAGTCAACT GATTGGAACA GGGAAATTAT CAGAAGATGA AGAAACCGAA 
GTGCAGGAAC AAATGAATCT CCTCAATTCA AGATGGGAAT GCCTCAGGGT AGCTAGCATG 
GAAAAACAAA GCAATTTACA TAAAGTTCTA ATGGATCTCC AGAATCAGCA ACTGAAAGAG 
TTAAATGACT GGCTAACCAA AACAGAAGAG AGAACAAGGA AAATGGAGAA GGAGCCCCTT 
GGACCTGATA TTGAAGACCT AAAACGCCAA GTACAACAAC ATAAGGTGCT TCAAGAAGAC 
TTAGAACAGG AACAAGTCAG GGTCAATTCC CTCACTCATA TGGTGGTGGT AGTCGATGAA 
TCTAGTGGAG ACCATGCAAC TGCTGCTTTG GAAGAACAAC TTAAGGTACT GGGAGATCGA 
TGGGCAAACA TCTGTAGGTG GACAGAAGAT CGCTGGGTTC TTTTACAAGA CATCCTCCTA 
AAATGGCAGC GTTTTACTGA AGAACAGTGC CTTTTCAGTG CATGGCTTTC GGAGAAGGAA 
GATGCAGTGA ACAAGATTCA CACAACTGGC TTTAAGGATC AAAGTGAAGT GTTATCAAAT 
CTTCAGAAAC TGGCTGTCTT AAAAACAGAT CTGGAAAAGA AGAAGCAAAC CATGGACAAA 
CTCTGCTCAC TCAACCAAGA CCTTCTTTCA GCGCTGAAAA ACACAGTGGT AGCCCACAAG 
ATGGAAGCAT GGCTGGACAA CTTTGCCCAG CGCTGGGATA ATTTAGTCCA GAAACTTGAA 
AAAAGTTCAG CACAGATTTC ACAGGCTGTC ACCACCACTC AGCCATCACT AACACAGACA 
ACTGTAATGG AAACAGTAAC TATGGTGACC ACGAGGGAAC ACATCTTGGT AAAGCATGCC 
CAAGAGGAAC TGCCACCACC ACCCCCTCAG AAGAAGAGGC AGATTATCGT GGATTCTGAA 
ATTAGGAAAA GGTTGGATGT CGATATAACT GAACTTCACA GTTGGATTAC TCGTTCAGAA 
GCTGTGTTGC AGAGTCCTGA ATTTGCAATC TATCGGAAGG AAGGCAACTT CTCAGACCTT 
AAAGAAAAAG TCAATGCCAT AGAGCGAGAA AAAGCCGAGA AGTTCAGAAA ACTGCAAGAT 
GCCAGCAGAT CAGCTCAGGC CCTGGTGGAA CAGATGGTGA ATGAGGGTGT TAATGCTGAC 
AGCATCAAAC AAGCCTCCGA ACAACTGAAC AGCCGGTGGA TAGAGTTCTG CCAATTGCTA 
AGCGAGAGAC TTAACTGGCT GGAGTATCAG AACAACATCA TCACTTTCTA TAATCAGCTA 
CAACAATTGG AGCAGATGAC AACTACTGCT GAAAACTGGT TGAAAACCCA GCCTACCACC 
ACATCAGAGC CAACAGCAAT TAAAAGCCAG TTAAAAATTT GTAAGGATGA AATCAACCGA 
CTGTCAGCTC TTCAGCCTCA AATCGAGCGA TTAAAAATTC AAAGCATAGC CCTGAAAGAG 
AAAGGACAAG GGCCAATGTT CCTGGATGCA GACTTTGTGG CCTTTACAAA TCATTTTAAC 
CAAGTCTTTG CTGATGTGCA GGCAAGAGAA AAAGAGCTAC AAACAATTTT TGACAGTTTG 
CCACCCATGC GCTATCAGGA GACTATGAGT ACCATCCTGA CATGGATCCA GCAGTCAGAA 
ACCAAACTCT CTATACCTCA GGTTACTGTC ACTGAATATG ACATCATGGA ACAGAGACTC 
GGAGAGCTAC AGGCTTTACA AAGCTCTCTG CAAGAGCAAC AAAATGGCCT AAACTATCTC 
AGCACCACTG TGAAAGAGAT GTCAAAGAAA GCACCACTGT CTGATATTAG TCGGAAATAT 
CAATCAGAAT TTGAAGAGAT TGAGGGACGT TGGAAGAAGC TGTCTTCCCA GCTGGTTGAA 
CATTGTCAAA AGTTGGAGGA GCAAATGGCT AAACTTCGAA AAATTCAGAA TCACATAAAA 
ACTCTGAAGA AATGGATCAC TGAAGTCGAT GTTTTCCTGA AGGAGGAATG GCCTGCCCTT 
GGGGATTCAG AAATTCTGAA AAGACAGCTG AAACAGTGCA GGCTTTTAGT CAATGACATT 
CAGACCATCC AGCCTAGTCT CAACAGTGTC AATGAAGGGG CTCAGAAGAT GAAGAATGAA 
GCAGAACCAG AGTTTGCTGG CAGACTTGAG ACAGAGCTCC GAGAACTTAA CACCCAGTGG 
GATTACATGT GCCGCCAGGT CTATGCCAGG AAGGAAGCCT TAAAAGGAGG TTTGGATAAA 
ACTGTAAGTC TTCAGAAAGA TCTGTCAGAG ATGCATGAGT GGATGACACA AGCTGAAGAA 
GAATACCTAG AGAGAGATTT CGAATACAAG ACCCCTGATG AATTACAGAC AGCAGTTGAA 
GAGATGAAGA GAGCTAAAGA AGAGGCCCAG CAAAAAGAAG CAAAAGTGAA ACTCCTAACC 
GAGTCCGTCA ATAGTGTCAT AGCTCAGGCT CCACCTGCAG CACAAGAGGC CTTAAAAAAG 
GAACTTGACA CTCTCACCAC CAACTACCAG TGGCTCTGCA CCAGGCTCAA TGGCAAATGC 
AAGACCTTGG AAGAAGTTTG GGCGTGCTGG CATGAGTTAT TGTCCTACTT GGAGAAGGCA 
AACAAGTGGC TAAGTGAAGT AGAAGTCAAG CTTAAAACCA CTGAAAATAT TTCTGGGGGA 
GCTGAGGAAA TCGCCGAGGT GCTTGATTCG CTTGAAAATT TGATGCAACA TTCAGAGGAT 
AACCCGAATC AGATTCGCAT ATTGGCACAG ACCTTGACAG ATGGTGGAGT CATGGATGAA 
CTGATCAATG AGGAGCTTGA GACATTTAAT TCTCGTTGGA GAGAACTCCA TGAAGAGGCT 
GTGAGGAGGC AAAAGTTGCT TGAGCAGAGT ATCCAGTCGG CCCAGGAGAT AGAAAAATCC 
TTGCACTTAA TTCAGGAGTC CCTCTCTTCC ATTGACAAGC AGTTGGCAGC TTATATTGCT 
GACAAAGTGG ATGCAGCTCA GATGCCTCAG GAAGCCCAGA AAATCCAATC AGATTTGACA 
AGTCATGAGA TCAGTTTAGA AGAAATGAAG AAACATAACC AGGGAAAGGA GACTGCCCAA 
AGGGTACTAT CCCAAATTGA TGTGGCACAG AAAAAATTGC AGGATGTTTC CATGAAGTTT 
CGATTATTCC AGAAGCCAGC CAATTTTGAG CAGCGCCTAC AAGAAAGTAA AATGATTTTA 
GATGAAGTGA AGATGCATTT ACCTGCGTTG GAAACAAAGA GTGTGGAACA GGAAGTAGTA 
CAGTCACAGT TAAATCATTG TGTGAACTTG TATAAAAGTC TGAGTGAAGT GAAGTCTGAA 
GTGGAAATGG TAATAAAAAC TGGACGTCAG ATTGTACAGA AGAAGCAGAC GGAAAACCCG 
AAAGAGCTTG ATGAAAGAGT TACAGCTTTG AAATTGCATT ATAATGAGCT GGGAGCAAAG 
GTGACAGAAA GAAAGCAACA GTTGGAAAAA TGCTTGAAAT TGTCCCGTAA GATGCGAAAG 
GAAATGAATG CCCTGACAGA ATGGCTGGCA GCGACAGATA TGGAACTGAC GAAGAGATCG 
GCAGTTGAAG GAATGCCTAG TAATTTGGAT TCTGAAGTTG CCTGGGGAAA GGCTACTCAG 
AAAGAGATTG AGAAACAGAA GGTTCACCTA AAGAGTGTCA CAGAGGTAGG AGAGGCCTTG 
AAAACGGTTT TGGGCAAGAA GGAAATGTTG GTGGAAGATA AACTGAGTCT TCTGAATAGT 
AACTGGATAG CCGTCACTTC CCGAGCAGAA GAGTGGTTAA ACCTTTTATT GGAATACCAG 
AAACACATGG AAAACTTTGA CCAGAATGTG GATTACATCA CAAACTGGAT CATTCAGGCT 
GATGCACTTT TGGATGAATC TGAGAAAAAG AAACCTCAGC AAAAAGAAGA CATACTTAAG 
CGTTTAAAGG CTGAAATGAA TGACATACGT CCAAAGGTGG ATTCTACACG TGACCAAGCA 
GCAAACCTGA TGGCAAACCG CGGCGACCAC TGCAGGAAAG TAGTAGAGCC CAAAATCTCA 
GAGCTCAACC ATCGATTTGC AGCCATTTCT CACAGAATTA AGACTGGAAA GGCCTCCATT 
CCTTTGAAGG AATTGGAGCA GTTTAACTCA GATATACAAA AATTGCTTGA ACCACTGGAG 
GCTGAAATTC AGCAGGGGGT GAATCTGAAA GAGGAAGACT TCAATAAAGA TATGAGTGAA 
GACAATGAGG GTACTGTAAA AGAATTGTTG CAAAGAGGAG ACAACTTACA ACAAAGAATC 
ACAGATGAGA GAAAGCGAGA GGAAATAAAG ATAAAACAAC AGCTGTTACA GACAAAACAT 
AATGCTCTCA AGGATTTGAG GTCTCAAAGA AGAAAAAAGG CTCTAGAAAT TTCTCACCAG 
TGGTATCAGT ACAAGAGGCA GGCTGATGAT CTCCTGAAAT GCTTGGATGA CATTGAAAAA 
AAATTAGCCA GCCTACCTGA ACCCAGAGAT GAAAGGAAAA TAAAGGAAAT TGATCGTGAA 
TTGCAGAAGA AGAAAGAGGA GCTGAATGCA GTGCGTAGGC AAGCTGAGGG CTTGTCTGAG 
GATGGGGCCG CAATGGCAGT GGAGCCAACT CAGATCCAGC TCAGCAAGCG CTGGCGGGAA 
ATTGAGAGCA AATTTGCTCA GTTTCGAAGA CTCAACTTTG CACAAATTCA CACTGTCCAT 
GAAGAGTCAG TGGTGGCGAT GACTGAAGAC ATGCCTTTGG AAATTTCTTA TGTGCCTTCT 
ACTTACCTGA CTGAGATCAC TCATGTCTCA CAAGCCCTAT CAGAAGTGGA AGAGCTTCTT 
AATGCTCCCG ACCTTTGTGC TCAAGATTTT GAAGATCTCT TTAAACAAGA GGAATCCTTG 
AAGAACATAA AAGACAGCCT GCAACAAATC TCAGGTCGGA TTGACATCAT TCACAATAAA 
AAGACAGCAG CATTGCACAG TGCCACTCCT GCAGAAAGGG CAAAGCTCCA GGAAGCTCTC 
TCACGGCTTG ATTTCCAATG GGAAAGAGTT AACAATATGT ACAAGGACCG ACAAGGGAGA 
TTTGACAGAT CTGTGGAAAA ATGGCGGCGG TTTCATTATG ATATGAAGAT ACTTAATCAA 
TGGCTAACAG AAGCTGAACA GTTTCTCAAA AAGACACAAA TTCCTGAGAA TTGGGAACAT 
GCCAAATACA AATGGTATCT TAAGGAACTC CAGGATGGCA TTGGACAGCG GCAAAGTGTT 
GTCAGGGTAT TGAATGCAAC TGGGGAAGAA ATAATTCAAC AGTCCTCAAA AACAGATGCC 
AGTATTCTCC AAGAAAAACT GGGAAGCCTG AATCTGCGGT GGCAGGAGGT CTGCAAACAG 
CTGGCAGAAA GAAAAAAGAG GCTAGAGGAA CAGAAGAATA TCTTGTCAGA ATTTCAAAGA 
GATGTAAATG AATTTGTTTT ATGGTTGGAA GAAGCGGATA ACGTTGCTAA TATTCCACTT 
GAACCTGGAA ATGAGCAGCA GCTAAAAGAA AAACTTGAAC AAGTCAAGTT ACTGGCAGAA 
GAGTTGCCCC TGCGCCAGGG AATTCTAAAA CAATTAAATG AAACTGGAGG AACAGTGCTT 
GTAAGTGCTC CCCTAAGCCC AGAAGAGCAA GATAAACTTG AAAATAAGCT CAAGCAGACA 
AATCTTCAGT GGATAAAGGT TTCTAGAAAT CTGCCTGAGA AGCAAGAAGA AATTGAGGCA 
CACGTAAAAG ACCTTGGACA GCTGGAAGAG CAGTTAAATC ATCTGCTTCT ATGGCTGTCT 
CCTATTAGGA ATCAGTTGGA AATTTACAAT CAGCCAAATC AAACAGGACC ATTTGACATC 
AAGGAAATTG AAGTAGCAGT TCAAGCTAAA CAGCCGGATG TGGAAGGGAT TTTGTCTAAA 
GGGCAGCATT TGTACAAGGA AAAACCAGCC ACTCAGCCAG CGAAGAGAAA GCTGGAAGAT 
CTCAGCTCTG ATTGGAAGGT GGTAACTCAG TTGCTTCAAG AGCTGCGGGC AAAGCAGCCT 
GGCCCAGCTC CTGGACTGAC CACTGTCAGA GCCCCTCCCA GTCAGACTGT TACTCTGGTG 
ACACAACCCG CGGTTACCAA GGAAACTGCC ATCTCCAAAC TAGAAATGCC ATCTTCATTG 
CTGTTGGAGG TACCTGCACT GGCAGATTTC AACCGAGCTT GGACAGAACT TACCGACTGG 
CTGTCTCTGC TTGATCGAGT TATAAAATCA CAGAGGGTGA TGGTGGGTGA TCTTGAAGAC 
ATTAACGAGA TGATCATCAA GCAGAAGGCA ACGCTGCAGG ATTTGGAACA GAGGCGCCCC 
CAGTTGGAAG AACTCATTAC CGCTGCCCAG AATTTGAAAA ACAAGACCAG CAATCAAGAG 
GCTAGAACAA TCATTACTGA TCGAATTGAA AGAATTCAGA GTCAGTGGGA TGAAGTACAG 
GAACATCTTC AGAACCGGAG GCAACAGTTG AATGAAATGT TAAAGGATTC CACACAATGG 
CTGGAAGCTA AAGAGGAGGC TGAGCAGGTG TTGGGGCAGG CCAGAGCCAA GCTTGAGTCA 
TGGAAGGAGG CTCCCTACAC AGTAGATGCA ATCCAAAAGA AAATCACAGA AACCAAGCAG 
TTGGCCAAAG ACCTCCGCCA GTGGCAGATA AATGTAGATG TTGCAAATGA TTTGGCACTG 
AAACTTCTCC GAGATTATTC TGCAGATGAT ACCAGAAAAG TACACATGAT AACAGAGAAC 
ATCAATGCCT CTTGGGCAAG CATCCATAAA AGATTGAGTG AGCGAGAGGC TGCTCTGGAA 
GAAACCCACA GATTACTGCA ACAGTTCCCC TTGGACCTGG AGAAGTTCCT TGCCTGGCTT 
ACAGAAGCCG AAACAACTGC CAACGTCCTG CAGGATGCCA CCCATAAGGA AAGGCTTCTA 
GAAGATTCCA AGGGAGTAAG AGAGCTGATG AAACAATGGC AAGATCTCCA AGGAGAAATC 
GAAGCTCACA CAGATATCTA TCACAACCTG GACGAAAATG GCCAAAAAGT CCTGAGATCC 
CTGGAAGGTT CTGACGATGC AGCCTTGTTG CAAAGACGTT TGGATAACAT GAACTTCAAG 
TGGAGCGAAC TTCGGAAAAA GTCTCTCAAC ATTAGGTCTC ACTTGGAAGC CAGTTCTGAC 
CAGTGGAAGC GTCTGCACCT TTCTCTTCAG GAACTTCTGG TATGGCTCCA GCTGAAAGAT 
GATGAGTTAA GCCGGCAGGC ACCCATTGGA GGAGACTTTC CAGCGGTGCA GAAGCAGAAT 
GATGTACACA GGGCCTTCAA GAGGGAATTG AAAACGAAAG AACCTGTAAT CATGAGTACT 
CTTGAGACTG TACGAATATT TCTGACAGAG CAGCCTTTAG AAGGACTAGA GAAACTCTAC 
CAGGAGCCCA GAGAGCTGCC TCCTGAAGAG AGAGCCCAGA ATGTCACACG GCTCCTACGA 
AAGCAAGCTG AGGAGGTCAA CACTCAGTGG GAAAAACTGA ACGTGCACTC TGCAGACTGG 
CAGAGAAAAA TAGACGAGGC CCTCGAAAGA CTCCAGGAGC TTCAGGAAGC AACAGATGAG 
CTGGATCTCA AACTACGTCA GGCAGAGGTG ATCAAAGGAT CCTGGCAGCC TGTGGGTGAT 
CTCCTCATTG ACTCTCTCCA AGATCACCTC GAAAAAGTCA AGGCGCTTCG AGGAGAAATT 
ACACCTCTGA AAGAGAATGT CAGCTACGTC AATGACCTTG CTCGCCAACT CACTACGTTG 
GGCATTCAGC TGTCACCATA TAACCTCAAC ACTCTGGAAG ACCTGAACAC CAGATGGAAG 
CTTCTGCAGG TGGCCATTGA GGACCGCATC AGGCAGCTGC ATGAAGCGCA CAGGGACTTT 
GGACCAGCCT CCCAGCACTT CCTTTCCACT TCTGTCCAGG GTCCCTGGGA GAGAGCCATC 
TCACCAAACA AAGTGCCCTA CTATATCAAC CACGAGACCC AAACAACTTG CTGGGACCAT 
CCCAAAATGA CAGAGCTCTA CCAGTCTTTA GCTGACCTGA ATAATGTCAG GTTCTCAGCT 
TACAGGACTG CCATGAAACT CCGAAGACTG CAGAAGGCCC TTTGCTTGGA TCTCTTGAGC 
CTATCGGCTG CATGCGATGC CTTGGACCAG CACAACCTCA AGCAAAATGA CCAGCCCATG 
GATATCCTGC AGGTCATTAA CTGTCTGACC ACTATTTATG ATCGCCTAGA GCAAGAGCAC 
AACAATCTGG TCAACGTCCC TCTCTGCGTG GATATGTGTC TCAATTGGCT GCTGAATGTT 
TATGACACGG GACGAACGGG GAGGATCCGG GTCCTGTCTT TTAAAACTGG CATCATTTCT 
CTGTGTAAAG CCCATTTGGA AGACAAGTAC AGATACCTCT TCAAGCAAGT GGCAAGTTCG 
ACAGGATTTT GTGACCAGCG CAGGCTGGGC CTCCTCCTGC ATGACTCTAT CCAGATCCCA 
AGACAGTTGG GTGAAGTCGC ATCGTTCGGG GGCAGTAACA TTGAGCCGAG TGTCAGGAGC 
TGCTTCCAGT TTGCTAATAA TAAGCCTGAG ATCGAAGCGG CCCTCTTCCT AGACTGGATG 
CGCCTGGAGC CCCAGTCCAT GGTGTGGCTG CCTGTCCTGC ACCGAGTGGC TGCCGCGGAA 
ACTGCCAAGC ACCAGGCCAA GTGCAACATC TGCAAGGAGT GTCCCATCAT CGGATTCAGG 
TACAGGAGTC TAAAGCACTT TAATTATGAC ATCTGCCAAA GTTGCTTTTT TTCTGGTCGA 
GTTGCAAAAG GCCATAAAAT GCACTATCCC ATGGTGGAAT ACTGCACTCC GACTACATCG 
GGAGAAGATG TCCGTGACTT TGCCAAGGTA CTAAAAAACA AATTTCGAAC CAAAAGGTAT 
TTTGCGAAGC ATCCCCGAAT GGGCTACCTG CCAGTGCAGA CTGTCTTAGA GGGGGACAAC 
ATGGAAACTC CTGTCACTCT GATCAACTTC TGGCCGGTAG ATTCTGCGCC TGCCTCGTCC 
CCTCAGCTTT CACACGATGA TACTCATTCA CGCATTGAGC ATTATGCTAG CAGGCTAGCA 
GAAATGGAAA ACAGCAATGG ATCTTATCTA AATGATAGCA TCTCTCCTAA TGAGAGCATA 
GATGATGAAC ATTTGTTAAT CCAGCATTAC TGCCAAAGTT TGAACCAGGA CTCCCCCCTG 
AGCCAGCCTC GTAGTCCTGC CCAGATCTTG ATTTCCTTAG AGAGTGAGGA AAGAGGGGAG 
CTAGAGAGAA TCCTAGCAGA TCTTGAGGAA GAAAACAGAA ATCTGCAAGC AGAGTATGAT 
CGTCTAAAGC AGCAGCATGA ACACAAAGGC CTGTCCCCAC TGCCATCCCC TCCTGAAATG 
ATGCCTACTT CTCCCCAAAG TCCCCGGGAT GCTGAGCTCA TCGCTGAGGC CAAGCTGCTG 
CGTCAACACA AAGGCCGCCT GGAAGCCAGG ATGCAAATCC TTGAAGACCA TAACAAACAA 
CTGGAATCCC AGTTACACAG GCTCAGGCAG CTGCTGGAGC AACCCCAGGC AGAGGCCAAG 
GTGAATGGTA CAACGGTGTC TTCTCCTTCT ACCTCTCTTC AGAGGTCAGA TAGCAGTCAG 
CCTATGCTGC TCCGGGTGGT CGGCAGTCAG ACTTCAGAAT CCATGGGCGA GGAAGACCTG 
CTCAGCCCTC CCCAGGACAC AAGCACAGGG TTAGAGGAAG TGATGGAGCA GCTCAACCAC 
TCCTTCCCTA GTTCCAGAGG ACACAATGTA GGAAGCCTTT TCCACTTGGC AGATGATTTG 
GGCAGAGCGA TGGAGTCCTT AGTTTCAGTC ATGACAGATG AAGAAGGGGC AGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMD ( XM_005641029.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005641086.1 Canis lupus familiaris dystrophin (DMD), transcript variant X1, mRNA.

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CloneID	OCe31973
Clone ID Related Accession (Same CDS sequence)	XM_005641029.3 , XM_005641029.2
Accession Version	XM_005641029.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	dystrophin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005641029.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTGGT GGGAAGAAGT AGAGGACTGT TATGAAAGAG AAGATGTTCA AAAGAAAACA 
TTCACAAAAT GGATAAATGC ACAGTTTTCT AAGTTTGGGA AGCAGCACAT AGAGAACCTC 
TTCAGTGACC TACAGGATGG GAGACGCCTC CTAGACCTTT TGGAAGGCCT GACAGGGCAA 
AAACTGCCAA AAGAAAAAGG ATCCACAAGA GTTCATGCCC TGAACAATGT CAACAAGGCA 
CTGCGCGTCT TGCAGAAAAA TAATGTTGAT TTAGTGAACA TTGGAAGTAC TGACATAGTA 
GATGGAAATC ACAAACTGAC TCTTGGTTTG ATTTGGAATA TAATCCTCCA CTGGCAGGTC 
AAAAATGTAA TGAAAAATAT CATGGCTGGA TTGCAACAAA CCAACAGTGA AAAGATTCTC 
CTGAGCTGGG TCCGACAATC AACTCGTAAT TATCCACAGG TTAATGTCAT TAACTTCACC 
ACCAGCTGGT CTGATGGCCT GGCTTTGAAC GCTCTCATCC ACAGTCATAG GCCAGACCTG 
TTTGATTGGA ATAGTGTGGT TTGCCAGCAG TCAGCCACAC AACGCCTGGA ACATGCATTC 
AACATTGCCA AATATCAATT AGGCATAGAG AAACTGCTTG ATCCTGAAGA TGTTGCCACC 
ACTTATCCAG ATAAGAAGTC CATCTTAATG TATATCACAT CACTCTTCCA AGTTTTGCCT 
CAACAAGTGA GCATTGAAGC CATCCAGGAA GTGGAAATGT TGCCAAGGCC ATCAAAAGTT 
ACTAGAGAAG AACATTTTCA ATTACATCAT CAAATGCACT ATTCTCAACA GATCACAGTC 
AGTCTAGCAC AGGGCTATGA ACGAGCCCCT TCCTCTCCTA AGCCTCGGTT CAAGAGCTAT 
GCCTACACAC AGGCTGCTTA TGTCACCACT TCTGACCCCA CACGGAGCCC ACTTCCTTCA 
CAGCATTTGG AAACTCCTGA AGACAAGTCA TTTGGCCGGT CATTGACAGA GACCGAAGCA 
AACCTGGACA GTTATCAAAC AGCTTTGGAA GAAGTACTCT CGTGGCTTCT TTCAGCTGAG 
GATGCACTGC AAGCCCAAGG AGAGATTTCT AATGATGTCG AAGAAGTGAA AGAACAATTT 
CATACTCATG AGGGATATAT GATGGACTTG ACATCCCATC AGGGACGGGT CGGTAATGTT 
CTCCAACTGG GAAGTCAACT GATTGGAACA GGGAAATTAT CAGAAGATGA AGAAACCGAA 
GTGCAGGAAC AAATGAATCT CCTCAATTCA AGATGGGAAT GCCTCAGGGT AGCTAGCATG 
GAAAAACAAA GCAATTTACA TAAAGTTCTA ATGGATCTCC AGAATCAGCA ACTGAAAGAG 
TTAAATGACT GGCTAACCAA AACAGAAGAG AGAACAAGGA AAATGGAGAA GGAGCCCCTT 
GGACCTGATA TTGAAGACCT AAAACGCCAA GTACAACAAC ATAAGGTGCT TCAAGAAGAC 
TTAGAACAGG AACAAGTCAG GGTCAATTCC CTCACTCATA TGGTGGTGGT AGTCGATGAA 
TCTAGTGGAG ACCATGCAAC TGCTGCTTTG GAAGAACAAC TTAAGGTACT GGGAGATCGA 
TGGGCAAACA TCTGTAGGTG GACAGAAGAT CGCTGGGTTC TTTTACAAGA CATCCTCCTA 
AAATGGCAGC GTTTTACTGA AGAACAGTGC CTTTTCAGTG CATGGCTTTC GGAGAAGGAA 
GATGCAGTGA ACAAGATTCA CACAACTGGC TTTAAGGATC AAAGTGAAGT GTTATCAAAT 
CTTCAGAAAC TGGCTGTCTT AAAAACAGAT CTGGAAAAGA AGAAGCAAAC CATGGACAAA 
CTCTGCTCAC TCAACCAAGA CCTTCTTTCA GCGCTGAAAA ACACAGTGGT AGCCCACAAG 
ATGGAAGCAT GGCTGGACAA CTTTGCCCAG CGCTGGGATA ATTTAGTCCA GAAACTTGAA 
AAAAGTTCAG CACAGATTTC ACAGGCTGTC ACCACCACTC AGCCATCACT AACACAGACA 
ACTGTAATGG AAACAGTAAC TATGGTGACC ACGAGGGAAC ACATCTTGGT AAAGCATGCC 
CAAGAGGAAC TGCCACCACC ACCCCCTCAG AAGAAGAGGC AGATTATCGT GGATTCTGAA 
ATTAGGAAAA GGTTGGATGT CGATATAACT GAACTTCACA GTTGGATTAC TCGTTCAGAA 
GCTGTGTTGC AGAGTCCTGA ATTTGCAATC TATCGGAAGG AAGGCAACTT CTCAGACCTT 
AAAGAAAAAG TCAATGCCAT AGAGCGAGAA AAAGCCGAGA AGTTCAGAAA ACTGCAAGAT 
GCCAGCAGAT CAGCTCAGGC CCTGGTGGAA CAGATGGTGA ATGAGGGTGT TAATGCTGAC 
AGCATCAAAC AAGCCTCCGA ACAACTGAAC AGCCGGTGGA TAGAGTTCTG CCAATTGCTA 
AGCGAGAGAC TTAACTGGCT GGAGTATCAG AACAACATCA TCACTTTCTA TAATCAGCTA 
CAACAATTGG AGCAGATGAC AACTACTGCT GAAAACTGGT TGAAAACCCA GCCTACCACC 
ACATCAGAGC CAACAGCAAT TAAAAGCCAG TTAAAAATTT GTAAGGATGA AATCAACCGA 
CTGTCAGCTC TTCAGCCTCA AATCGAGCGA TTAAAAATTC AAAGCATAGC CCTGAAAGAG 
AAAGGACAAG GGCCAATGTT CCTGGATGCA GACTTTGTGG CCTTTACAAA TCATTTTAAC 
CAAGTCTTTG CTGATGTGCA GGCAAGAGAA AAAGAGCTAC AAACAATTTT TGACAGTTTG 
CCACCCATGC GCTATCAGGA GACTATGAGT ACCATCCTGA CATGGATCCA GCAGTCAGAA 
ACCAAACTCT CTATACCTCA GGTTACTGTC ACTGAATATG ACATCATGGA ACAGAGACTC 
GGAGAGCTAC AGGCTTTACA AAGCTCTCTG CAAGAGCAAC AAAATGGCCT AAACTATCTC 
AGCACCACTG TGAAAGAGAT GTCAAAGAAA GCACCACTGT CTGATATTAG TCGGAAATAT 
CAATCAGAAT TTGAAGAGAT TGAGGGACGT TGGAAGAAGC TGTCTTCCCA GCTGGTTGAA 
CATTGTCAAA AGTTGGAGGA GCAAATGGCT AAACTTCGAA AAATTCAGAA TCACATAAAA 
ACTCTGAAGA AATGGATCAC TGAAGTCGAT GTTTTCCTGA AGGAGGAATG GCCTGCCCTT 
GGGGATTCAG AAATTCTGAA AAGACAGCTG AAACAGTGCA GGCTTTTAGT CAATGACATT 
CAGACCATCC AGCCTAGTCT CAACAGTGTC AATGAAGGGG CTCAGAAGAT GAAGAATGAA 
GCAGAACCAG AGTTTGCTGG CAGACTTGAG ACAGAGCTCC GAGAACTTAA CACCCAGTGG 
GATTACATGT GCCGCCAGGT CTATGCCAGG AAGGAAGCCT TAAAAGGAGG TTTGGATAAA 
ACTGTAAGTC TTCAGAAAGA TCTGTCAGAG ATGCATGAGT GGATGACACA AGCTGAAGAA 
GAATACCTAG AGAGAGATTT CGAATACAAG ACCCCTGATG AATTACAGAC AGCAGTTGAA 
GAGATGAAGA GAGCTAAAGA AGAGGCCCAG CAAAAAGAAG CAAAAGTGAA ACTCCTAACC 
GAGTCCGTCA ATAGTGTCAT AGCTCAGGCT CCACCTGCAG CACAAGAGGC CTTAAAAAAG 
GAACTTGACA CTCTCACCAC CAACTACCAG TGGCTCTGCA CCAGGCTCAA TGGCAAATGC 
AAGACCTTGG AAGAAGTTTG GGCGTGCTGG CATGAGTTAT TGTCCTACTT GGAGAAGGCA 
AACAAGTGGC TAAGTGAAGT AGAAGTCAAG CTTAAAACCA CTGAAAATAT TTCTGGGGGA 
GCTGAGGAAA TCGCCGAGGT GCTTGATTCG CTTGAAAATT TGATGCAACA TTCAGAGGAT 
AACCCGAATC AGATTCGCAT ATTGGCACAG ACCTTGACAG ATGGTGGAGT CATGGATGAA 
CTGATCAATG AGGAGCTTGA GACATTTAAT TCTCGTTGGA GAGAACTCCA TGAAGAGGCT 
GTGAGGAGGC AAAAGTTGCT TGAGCAGAGT ATCCAGTCGG CCCAGGAGAT AGAAAAATCC 
TTGCACTTAA TTCAGGAGTC CCTCTCTTCC ATTGACAAGC AGTTGGCAGC TTATATTGCT 
GACAAAGTGG ATGCAGCTCA GATGCCTCAG GAAGCCCAGA AAATCCAATC AGATTTGACA 
AGTCATGAGA TCAGTTTAGA AGAAATGAAG AAACATAACC AGGGAAAGGA GACTGCCCAA 
AGGGTACTAT CCCAAATTGA TGTGGCACAG AAAAAATTGC AGGATGTTTC CATGAAGTTT 
CGATTATTCC AGAAGCCAGC CAATTTTGAG CAGCGCCTAC AAGAAAGTAA AATGATTTTA 
GATGAAGTGA AGATGCATTT ACCTGCGTTG GAAACAAAGA GTGTGGAACA GGAAGTAGTA 
CAGTCACAGT TAAATCATTG TGTGAACTTG TATAAAAGTC TGAGTGAAGT GAAGTCTGAA 
GTGGAAATGG TAATAAAAAC TGGACGTCAG ATTGTACAGA AGAAGCAGAC GGAAAACCCG 
AAAGAGCTTG ATGAAAGAGT TACAGCTTTG AAATTGCATT ATAATGAGCT GGGAGCAAAG 
GTGACAGAAA GAAAGCAACA GTTGGAAAAA TGCTTGAAAT TGTCCCGTAA GATGCGAAAG 
GAAATGAATG CCCTGACAGA ATGGCTGGCA GCGACAGATA TGGAACTGAC GAAGAGATCG 
GCAGTTGAAG GAATGCCTAG TAATTTGGAT TCTGAAGTTG CCTGGGGAAA GGCTACTCAG 
AAAGAGATTG AGAAACAGAA GGTTCACCTA AAGAGTGTCA CAGAGGTAGG AGAGGCCTTG 
AAAACGGTTT TGGGCAAGAA GGAAATGTTG GTGGAAGATA AACTGAGTCT TCTGAATAGT 
AACTGGATAG CCGTCACTTC CCGAGCAGAA GAGTGGTTAA ACCTTTTATT GGAATACCAG 
AAACACATGG AAAACTTTGA CCAGAATGTG GATTACATCA CAAACTGGAT CATTCAGGCT 
GATGCACTTT TGGATGAATC TGAGAAAAAG AAACCTCAGC AAAAAGAAGA CATACTTAAG 
CGTTTAAAGG CTGAAATGAA TGACATACGT CCAAAGGTGG ATTCTACACG TGACCAAGCA 
GCAAACCTGA TGGCAAACCG CGGCGACCAC TGCAGGAAAG TAGTAGAGCC CAAAATCTCA 
GAGCTCAACC ATCGATTTGC AGCCATTTCT CACAGAATTA AGACTGGAAA GGCCTCCATT 
CCTTTGAAGG AATTGGAGCA GTTTAACTCA GATATACAAA AATTGCTTGA ACCACTGGAG 
GCTGAAATTC AGCAGGGGGT GAATCTGAAA GAGGAAGACT TCAATAAAGA TATGAGTGAA 
GACAATGAGG GTACTGTAAA AGAATTGTTG CAAAGAGGAG ACAACTTACA ACAAAGAATC 
ACAGATGAGA GAAAGCGAGA GGAAATAAAG ATAAAACAAC AGCTGTTACA GACAAAACAT 
AATGCTCTCA AGGATTTGAG GTCTCAAAGA AGAAAAAAGG CTCTAGAAAT TTCTCACCAG 
TGGTATCAGT ACAAGAGGCA GGCTGATGAT CTCCTGAAAT GCTTGGATGA CATTGAAAAA 
AAATTAGCCA GCCTACCTGA ACCCAGAGAT GAAAGGAAAA TAAAGGAAAT TGATCGTGAA 
TTGCAGAAGA AGAAAGAGGA GCTGAATGCA GTGCGTAGGC AAGCTGAGGG CTTGTCTGAG 
GATGGGGCCG CAATGGCAGT GGAGCCAACT CAGATCCAGC TCAGCAAGCG CTGGCGGGAA 
ATTGAGAGCA AATTTGCTCA GTTTCGAAGA CTCAACTTTG CACAAATTCA CACTGTCCAT 
GAAGAGTCAG TGGTGGCGAT GACTGAAGAC ATGCCTTTGG AAATTTCTTA TGTGCCTTCT 
ACTTACCTGA CTGAGATCAC TCATGTCTCA CAAGCCCTAT CAGAAGTGGA AGAGCTTCTT 
AATGCTCCCG ACCTTTGTGC TCAAGATTTT GAAGATCTCT TTAAACAAGA GGAATCCTTG 
AAGAACATAA AAGACAGCCT GCAACAAATC TCAGGTCGGA TTGACATCAT TCACAATAAA 
AAGACAGCAG CATTGCACAG TGCCACTCCT GCAGAAAGGG CAAAGCTCCA GGAAGCTCTC 
TCACGGCTTG ATTTCCAATG GGAAAGAGTT AACAATATGT ACAAGGACCG ACAAGGGAGA 
TTTGACAGAT CTGTGGAAAA ATGGCGGCGG TTTCATTATG ATATGAAGAT ACTTAATCAA 
TGGCTAACAG AAGCTGAACA GTTTCTCAAA AAGACACAAA TTCCTGAGAA TTGGGAACAT 
GCCAAATACA AATGGTATCT TAAGGAACTC CAGGATGGCA TTGGACAGCG GCAAAGTGTT 
GTCAGGGTAT TGAATGCAAC TGGGGAAGAA ATAATTCAAC AGTCCTCAAA AACAGATGCC 
AGTATTCTCC AAGAAAAACT GGGAAGCCTG AATCTGCGGT GGCAGGAGGT CTGCAAACAG 
CTGGCAGAAA GAAAAAAGAG GCTAGAGGAA CAGAAGAATA TCTTGTCAGA ATTTCAAAGA 
GATGTAAATG AATTTGTTTT ATGGTTGGAA GAAGCGGATA ACGTTGCTAA TATTCCACTT 
GAACCTGGAA ATGAGCAGCA GCTAAAAGAA AAACTTGAAC AAGTCAAGTT ACTGGCAGAA 
GAGTTGCCCC TGCGCCAGGG AATTCTAAAA CAATTAAATG AAACTGGAGG AACAGTGCTT 
GTAAGTGCTC CCCTAAGCCC AGAAGAGCAA GATAAACTTG AAAATAAGCT CAAGCAGACA 
AATCTTCAGT GGATAAAGGT TTCTAGAAAT CTGCCTGAGA AGCAAGAAGA AATTGAGGCA 
CACGTAAAAG ACCTTGGACA GCTGGAAGAG CAGTTAAATC ATCTGCTTCT ATGGCTGTCT 
CCTATTAGGA ATCAGTTGGA AATTTACAAT CAGCCAAATC AAACAGGACC ATTTGACATC 
AAGGAAATTG AAGTAGCAGT TCAAGCTAAA CAGCCGGATG TGGAAGGGAT TTTGTCTAAA 
GGGCAGCATT TGTACAAGGA AAAACCAGCC ACTCAGCCAG CGAAGAGAAA GCTGGAAGAT 
CTCAGCTCTG ATTGGAAGGT GGTAACTCAG TTGCTTCAAG AGCTGCGGGC AAAGCAGCCT 
GGCCCAGCTC CTGGACTGAC CACTGTCAGA GCCCCTCCCA GTCAGACTGT TACTCTGGTG 
ACACAACCCG CGGTTACCAA GGAAACTGCC ATCTCCAAAC TAGAAATGCC ATCTTCATTG 
CTGTTGGAGG TACCTGCACT GGCAGATTTC AACCGAGCTT GGACAGAACT TACCGACTGG 
CTGTCTCTGC TTGATCGAGT TATAAAATCA CAGAGGGTGA TGGTGGGTGA TCTTGAAGAC 
ATTAACGAGA TGATCATCAA GCAGAAGGCA ACGCTGCAGG ATTTGGAACA GAGGCGCCCC 
CAGTTGGAAG AACTCATTAC CGCTGCCCAG AATTTGAAAA ACAAGACCAG CAATCAAGAG 
GCTAGAACAA TCATTACTGA TCGAATTGAA AGAATTCAGA GTCAGTGGGA TGAAGTACAG 
GAACATCTTC AGAACCGGAG GCAACAGTTG AATGAAATGT TAAAGGATTC CACACAATGG 
CTGGAAGCTA AAGAGGAGGC TGAGCAGGTG TTGGGGCAGG CCAGAGCCAA GCTTGAGTCA 
TGGAAGGAGG CTCCCTACAC AGTAGATGCA ATCCAAAAGA AAATCACAGA AACCAAGCAG 
TTGGCCAAAG ACCTCCGCCA GTGGCAGATA AATGTAGATG TTGCAAATGA TTTGGCACTG 
AAACTTCTCC GAGATTATTC TGCAGATGAT ACCAGAAAAG TACACATGAT AACAGAGAAC 
ATCAATGCCT CTTGGGCAAG CATCCATAAA AGATTGAGTG AGCGAGAGGC TGCTCTGGAA 
GAAACCCACA GATTACTGCA ACAGTTCCCC TTGGACCTGG AGAAGTTCCT TGCCTGGCTT 
ACAGAAGCCG AAACAACTGC CAACGTCCTG CAGGATGCCA CCCATAAGGA AAGGCTTCTA 
GAAGATTCCA AGGGAGTAAG AGAGCTGATG AAACAATGGC AAGATCTCCA AGGAGAAATC 
GAAGCTCACA CAGATATCTA TCACAACCTG GACGAAAATG GCCAAAAAGT CCTGAGATCC 
CTGGAAGGTT CTGACGATGC AGCCTTGTTG CAAAGACGTT TGGATAACAT GAACTTCAAG 
TGGAGCGAAC TTCGGAAAAA GTCTCTCAAC ATTAGGTCTC ACTTGGAAGC CAGTTCTGAC 
CAGTGGAAGC GTCTGCACCT TTCTCTTCAG GAACTTCTGG TATGGCTCCA GCTGAAAGAT 
GATGAGTTAA GCCGGCAGGC ACCCATTGGA GGAGACTTTC CAGCGGTGCA GAAGCAGAAT 
GATGTACACA GGGCCTTCAA GAGGGAATTG AAAACGAAAG AACCTGTAAT CATGAGTACT 
CTTGAGACTG TACGAATATT TCTGACAGAG CAGCCTTTAG AAGGACTAGA GAAACTCTAC 
CAGGAGCCCA GAGAGCTGCC TCCTGAAGAG AGAGCCCAGA ATGTCACACG GCTCCTACGA 
AAGCAAGCTG AGGAGGTCAA CACTCAGTGG GAAAAACTGA ACGTGCACTC TGCAGACTGG 
CAGAGAAAAA TAGACGAGGC CCTCGAAAGA CTCCAGGAGC TTCAGGAAGC AACAGATGAG 
CTGGATCTCA AACTACGTCA GGCAGAGGTG ATCAAAGGAT CCTGGCAGCC TGTGGGTGAT 
CTCCTCATTG ACTCTCTCCA AGATCACCTC GAAAAAGTCA AGGCGCTTCG AGGAGAAATT 
ACACCTCTGA AAGAGAATGT CAGCTACGTC AATGACCTTG CTCGCCAACT CACTACGTTG 
GGCATTCAGC TGTCACCATA TAACCTCAAC ACTCTGGAAG ACCTGAACAC CAGATGGAAG 
CTTCTGCAGG TGGCCATTGA GGACCGCATC AGGCAGCTGC ATGAAGCGCA CAGGGACTTT 
GGACCAGCCT CCCAGCACTT CCTTTCCACT TCTGTCCAGG GTCCCTGGGA GAGAGCCATC 
TCACCAAACA AAGTGCCCTA CTATATCAAC CACGAGACCC AAACAACTTG CTGGGACCAT 
CCCAAAATGA CAGAGCTCTA CCAGTCTTTA GCTGACCTGA ATAATGTCAG GTTCTCAGCT 
TACAGGACTG CCATGAAACT CCGAAGACTG CAGAAGGCCC TTTGCTTGGA TCTCTTGAGC 
CTATCGGCTG CATGCGATGC CTTGGACCAG CACAACCTCA AGCAAAATGA CCAGCCCATG 
GATATCCTGC AGGTCATTAA CTGTCTGACC ACTATTTATG ATCGCCTAGA GCAAGAGCAC 
AACAATCTGG TCAACGTCCC TCTCTGCGTG GATATGTGTC TCAATTGGCT GCTGAATGTT 
TATGACACGG GACGAACGGG GAGGATCCGG GTCCTGTCTT TTAAAACTGG CATCATTTCT 
CTGTGTAAAG CCCATTTGGA AGACAAGTAC AGATACCTCT TCAAGCAAGT GGCAAGTTCG 
ACAGGATTTT GTGACCAGCG CAGGCTGGGC CTCCTCCTGC ATGACTCTAT CCAGATCCCA 
AGACAGTTGG GTGAAGTCGC ATCGTTCGGG GGCAGTAACA TTGAGCCGAG TGTCAGGAGC 
TGCTTCCAGT TTGCTAATAA TAAGCCTGAG ATCGAAGCGG CCCTCTTCCT AGACTGGATG 
CGCCTGGAGC CCCAGTCCAT GGTGTGGCTG CCTGTCCTGC ACCGAGTGGC TGCCGCGGAA 
ACTGCCAAGC ACCAGGCCAA GTGCAACATC TGCAAGGAGT GTCCCATCAT CGGATTCAGG 
TACAGGAGTC TAAAGCACTT TAATTATGAC ATCTGCCAAA GTTGCTTTTT TTCTGGTCGA 
GTTGCAAAAG GCCATAAAAT GCACTATCCC ATGGTGGAAT ACTGCACTCC GACTACATCG 
GGAGAAGATG TCCGTGACTT TGCCAAGGTA CTAAAAAACA AATTTCGAAC CAAAAGGTAT 
TTTGCGAAGC ATCCCCGAAT GGGCTACCTG CCAGTGCAGA CTGTCTTAGA GGGGGACAAC 
ATGGAAACTC CTGTCACTCT GATCAACTTC TGGCCGGTAG ATTCTGCGCC TGCCTCGTCC 
CCTCAGCTTT CACACGATGA TACTCATTCA CGCATTGAGC ATTATGCTAG CAGGCTAGCA 
GAAATGGAAA ACAGCAATGG ATCTTATCTA AATGATAGCA TCTCTCCTAA TGAGAGCATA 
GATGATGAAC ATTTGTTAAT CCAGCATTAC TGCCAAAGTT TGAACCAGGA CTCCCCCCTG 
AGCCAGCCTC GTAGTCCTGC CCAGATCTTG ATTTCCTTAG AGAGTGAGGA AAGAGGGGAG 
CTAGAGAGAA TCCTAGCAGA TCTTGAGGAA GAAAACAGAA ATCTGCAAGC AGAGTATGAT 
CGTCTAAAGC AGCAGCATGA ACACAAAGGC CTGTCCCCAC TGCCATCCCC TCCTGAAATG 
ATGCCTACTT CTCCCCAAAG TCCCCGGGAT GCTGAGCTCA TCGCTGAGGC CAAGCTGCTG 
CGTCAACACA AAGGCCGCCT GGAAGCCAGG ATGCAAATCC TTGAAGACCA TAACAAACAA 
CTGGAATCCC AGTTACACAG GCTCAGGCAG CTGCTGGAGC AACCCCAGGC AGAGGCCAAG 
GTGAATGGTA CAACGGTGTC TTCTCCTTCT ACCTCTCTTC AGAGGTCAGA TAGCAGTCAG 
CCTATGCTGC TCCGGGTGGT CGGCAGTCAG ACTTCAGAAT CCATGGGCGA GGAAGACCTG 
CTCAGCCCTC CCCAGGACAC AAGCACAGGG TTAGAGGAAG TGATGGAGCA GCTCAACCAC 
TCCTTCCCTA GTTCCAGAGG ACACAATGTA GGAAGCCTTT TCCACTTGGC AGATGATTTG 
GGCAGAGCGA TGGAGTCCTT AGTTTCAGTC ATGACAGATG AAGAAGGGGC AGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005641086.1
CDS	248..11344
Translation	MLWWEEVEDCYEREDVQKKTFTKWINAQFSKFGKQHIENLFSDLQDGRRLLDLLEGLTGQKLPKEKGSTRVHALNNVNKALRVLQKNNVDLVNIGSTDIVDGNHKLTLGLIWNIILHWQVKNVMKNIMAGLQQTNSEKILLSWVRQSTRNYPQVNVINFTTSWSDGLALNALIHSHRPDLFDWNSVVCQQSATQRLEHAFNIAKYQLGIEKLLDPEDVATTYPDKKSILMYITSLFQVLPQQVSIEAIQEVEMLPRPSKVTREEHFQLHHQMHYSQQITVSLAQGYERAPSSPKPRFKSYAYTQAAYVTTSDPTRSPLPSQHLETPEDKSFGRSLTETEANLDSYQTALEEVLSWLLSAEDALQAQGEISNDVEEVKEQFHTHEGYMMDLTSHQGRVGNVLQLGSQLIGTGKLSEDEETEVQEQMNLLNSRWECLRVASMEKQSNLHKVLMDLQNQQLKELNDWLTKTEERTRKMEKEPLGPDIEDLKRQVQQHKVLQEDLEQEQVRVNSLTHMVVVVDESSGDHATAALEEQLKVLGDRWANICRWTEDRWVLLQDILLKWQRFTEEQCLFSAWLSEKEDAVNKIHTTGFKDQSEVLSNLQKLAVLKTDLEKKKQTMDKLCSLNQDLLSALKNTVVAHKMEAWLDNFAQRWDNLVQKLEKSSAQISQAVTTTQPSLTQTTVMETVTMVTTREHILVKHAQEELPPPPPQKKRQIIVDSEIRKRLDVDITELHSWITRSEAVLQSPEFAIYRKEGNFSDLKEKVNAIEREKAEKFRKLQDASRSAQALVEQMVNEGVNADSIKQASEQLNSRWIEFCQLLSERLNWLEYQNNIITFYNQLQQLEQMTTTAENWLKTQPTTTSEPTAIKSQLKICKDEINRLSALQPQIERLKIQSIALKEKGQGPMFLDADFVAFTNHFNQVFADVQAREKELQTIFDSLPPMRYQETMSTILTWIQQSETKLSIPQVTVTEYDIMEQRLGELQALQSSLQEQQNGLNYLSTTVKEMSKKAPLSDISRKYQSEFEEIEGRWKKLSSQLVEHCQKLEEQMAKLRKIQNHIKTLKKWITEVDVFLKEEWPALGDSEILKRQLKQCRLLVNDIQTIQPSLNSVNEGAQKMKNEAEPEFAGRLETELRELNTQWDYMCRQVYARKEALKGGLDKTVSLQKDLSEMHEWMTQAEEEYLERDFEYKTPDELQTAVEEMKRAKEEAQQKEAKVKLLTESVNSVIAQAPPAAQEALKKELDTLTTNYQWLCTRLNGKCKTLEEVWACWHELLSYLEKANKWLSEVEVKLKTTENISGGAEEIAEVLDSLENLMQHSEDNPNQIRILAQTLTDGGVMDELINEELETFNSRWRELHEEAVRRQKLLEQSIQSAQEIEKSLHLIQESLSSIDKQLAAYIADKVDAAQMPQEAQKIQSDLTSHEISLEEMKKHNQGKETAQRVLSQIDVAQKKLQDVSMKFRLFQKPANFEQRLQESKMILDEVKMHLPALETKSVEQEVVQSQLNHCVNLYKSLSEVKSEVEMVIKTGRQIVQKKQTENPKELDERVTALKLHYNELGAKVTERKQQLEKCLKLSRKMRKEMNALTEWLAATDMELTKRSAVEGMPSNLDSEVAWGKATQKEIEKQKVHLKSVTEVGEALKTVLGKKEMLVEDKLSLLNSNWIAVTSRAEEWLNLLLEYQKHMENFDQNVDYITNWIIQADALLDESEKKKPQQKEDILKRLKAEMNDIRPKVDSTRDQAANLMANRGDHCRKVVEPKISELNHRFAAISHRIKTGKASIPLKELEQFNSDIQKLLEPLEAEIQQGVNLKEEDFNKDMSEDNEGTVKELLQRGDNLQQRITDERKREEIKIKQQLLQTKHNALKDLRSQRRKKALEISHQWYQYKRQADDLLKCLDDIEKKLASLPEPRDERKIKEIDRELQKKKEELNAVRRQAEGLSEDGAAMAVEPTQIQLSKRWREIESKFAQFRRLNFAQIHTVHEESVVAMTEDMPLEISYVPSTYLTEITHVSQALSEVEELLNAPDLCAQDFEDLFKQEESLKNIKDSLQQISGRIDIIHNKKTAALHSATPAERAKLQEALSRLDFQWERVNNMYKDRQGRFDRSVEKWRRFHYDMKILNQWLTEAEQFLKKTQIPENWEHAKYKWYLKELQDGIGQRQSVVRVLNATGEEIIQQSSKTDASILQEKLGSLNLRWQEVCKQLAERKKRLEEQKNILSEFQRDVNEFVLWLEEADNVANIPLEPGNEQQLKEKLEQVKLLAEELPLRQGILKQLNETGGTVLVSAPLSPEEQDKLENKLKQTNLQWIKVSRNLPEKQEEIEAHVKDLGQLEEQLNHLLLWLSPIRNQLEIYNQPNQTGPFDIKEIEVAVQAKQPDVEGILSKGQHLYKEKPATQPAKRKLEDLSSDWKVVTQLLQELRAKQPGPAPGLTTVRAPPSQTVTLVTQPAVTKETAISKLEMPSSLLLEVPALADFNRAWTELTDWLSLLDRVIKSQRVMVGDLEDINEMIIKQKATLQDLEQRRPQLEELITAAQNLKNKTSNQEARTIITDRIERIQSQWDEVQEHLQNRRQQLNEMLKDSTQWLEAKEEAEQVLGQARAKLESWKEAPYTVDAIQKKITETKQLAKDLRQWQINVDVANDLALKLLRDYSADDTRKVHMITENINASWASIHKRLSEREAALEETHRLLQQFPLDLEKFLAWLTEAETTANVLQDATHKERLLEDSKGVRELMKQWQDLQGEIEAHTDIYHNLDENGQKVLRSLEGSDDAALLQRRLDNMNFKWSELRKKSLNIRSHLEASSDQWKRLHLSLQELLVWLQLKDDELSRQAPIGGDFPAVQKQNDVHRAFKRELKTKEPVIMSTLETVRIFLTEQPLEGLEKLYQEPRELPPEERAQNVTRLLRKQAEEVNTQWEKLNVHSADWQRKIDEALERLQELQEATDELDLKLRQAEVIKGSWQPVGDLLIDSLQDHLEKVKALRGEITPLKENVSYVNDLARQLTTLGIQLSPYNLNTLEDLNTRWKLLQVAIEDRIRQLHEAHRDFGPASQHFLSTSVQGPWERAISPNKVPYYINHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQVINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPVTLINFWPVDSAPASSPQLSHDDTHSRIEHYASRLAEMENSNGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKQQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDTSTGLEEVMEQLNHSFPSSRGHNVGSLFHLADDLGRAMESLVSVMTDEEGAE

Target ORF information:

RefSeq Version	XM_005641029.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris dystrophin (DMD), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005641029.2

ORF Insert Sequence:

ATGCTTTGGT GGGAAGAAGT AGAGGACTGT TATGAAAGAG AAGATGTTCA AAAGAAAACA 
TTCACAAAAT GGATAAATGC ACAGTTTTCT AAGTTTGGGA AGCAGCACAT AGAGAACCTC 
TTCAGTGACC TACAGGATGG GAGACGCCTC CTAGACCTTT TGGAAGGCCT GACAGGGCAA 
AAACTGCCAA AAGAAAAAGG ATCCACAAGA GTTCATGCCC TGAACAATGT CAACAAGGCA 
CTGCGCGTCT TGCAGAAAAA TAATGTTGAT TTAGTGAACA TTGGAAGTAC TGACATAGTA 
GATGGAAATC ACAAACTGAC TCTTGGTTTG ATTTGGAATA TAATCCTCCA CTGGCAGGTC 
AAAAATGTAA TGAAAAATAT CATGGCTGGA TTGCAACAAA CCAACAGTGA AAAGATTCTC 
CTGAGCTGGG TCCGACAATC AACTCGTAAT TATCCACAGG TTAATGTCAT TAACTTCACC 
ACCAGCTGGT CTGATGGCCT GGCTTTGAAC GCTCTCATCC ACAGTCATAG GCCAGACCTG 
TTTGATTGGA ATAGTGTGGT TTGCCAGCAG TCAGCCACAC AACGCCTGGA ACATGCATTC 
AACATTGCCA AATATCAATT AGGCATAGAG AAACTGCTTG ATCCTGAAGA TGTTGCCACC 
ACTTATCCAG ATAAGAAGTC CATCTTAATG TATATCACAT CACTCTTCCA AGTTTTGCCT 
CAACAAGTGA GCATTGAAGC CATCCAGGAA GTGGAAATGT TGCCAAGGCC ATCAAAAGTT 
ACTAGAGAAG AACATTTTCA ATTACATCAT CAAATGCACT ATTCTCAACA GATCACAGTC 
AGTCTAGCAC AGGGCTATGA ACGAGCCCCT TCCTCTCCTA AGCCTCGGTT CAAGAGCTAT 
GCCTACACAC AGGCTGCTTA TGTCACCACT TCTGACCCCA CACGGAGCCC ACTTCCTTCA 
CAGCATTTGG AAACTCCTGA AGACAAGTCA TTTGGCCGGT CATTGACAGA GACCGAAGCA 
AACCTGGACA GTTATCAAAC AGCTTTGGAA GAAGTACTCT CGTGGCTTCT TTCAGCTGAG 
GATGCACTGC AAGCCCAAGG AGAGATTTCT AATGATGTCG AAGAAGTGAA AGAACAATTT 
CATACTCATG AGGGATATAT GATGGACTTG ACATCCCATC AGGGACGGGT CGGTAATGTT 
CTCCAACTGG GAAGTCAACT GATTGGAACA GGGAAATTAT CAGAAGATGA AGAAACCGAA 
GTGCAGGAAC AAATGAATCT CCTCAATTCA AGATGGGAAT GCCTCAGGGT AGCTAGCATG 
GAAAAACAAA GCAATTTACA TAAAGTTCTA ATGGATCTCC AGAATCAGCA ACTGAAAGAG 
TTAAATGACT GGCTAACCAA AACAGAAGAG AGAACAAGGA AAATGGAGAA GGAGCCCCTT 
GGACCTGATA TTGAAGACCT AAAACGCCAA GTACAACAAC ATAAGGTGCT TCAAGAAGAC 
TTAGAACAGG AACAAGTCAG GGTCAATTCC CTCACTCATA TGGTGGTGGT AGTCGATGAA 
TCTAGTGGAG ACCATGCAAC TGCTGCTTTG GAAGAACAAC TTAAGGTACT GGGAGATCGA 
TGGGCAAACA TCTGTAGGTG GACAGAAGAT CGCTGGGTTC TTTTACAAGA CATCCTCCTA 
AAATGGCAGC GTTTTACTGA AGAACAGTGC CTTTTCAGTG CATGGCTTTC GGAGAAGGAA 
GATGCAGTGA ACAAGATTCA CACAACTGGC TTTAAGGATC AAAGTGAAGT GTTATCAAAT 
CTTCAGAAAC TGGCTGTCTT AAAAACAGAT CTGGAAAAGA AGAAGCAAAC CATGGACAAA 
CTCTGCTCAC TCAACCAAGA CCTTCTTTCA GCGCTGAAAA ACACAGTGGT AGCCCACAAG 
ATGGAAGCAT GGCTGGACAA CTTTGCCCAG CGCTGGGATA ATTTAGTCCA GAAACTTGAA 
AAAAGTTCAG CACAGATTTC ACAGGCTGTC ACCACCACTC AGCCATCACT AACACAGACA 
ACTGTAATGG AAACAGTAAC TATGGTGACC ACGAGGGAAC ACATCTTGGT AAAGCATGCC 
CAAGAGGAAC TGCCACCACC ACCCCCTCAG AAGAAGAGGC AGATTATCGT GGATTCTGAA 
ATTAGGAAAA GGTTGGATGT CGATATAACT GAACTTCACA GTTGGATTAC TCGTTCAGAA 
GCTGTGTTGC AGAGTCCTGA ATTTGCAATC TATCGGAAGG AAGGCAACTT CTCAGACCTT 
AAAGAAAAAG TCAATGCCAT AGAGCGAGAA AAAGCCGAGA AGTTCAGAAA ACTGCAAGAT 
GCCAGCAGAT CAGCTCAGGC CCTGGTGGAA CAGATGGTGA ATGAGGGTGT TAATGCTGAC 
AGCATCAAAC AAGCCTCCGA ACAACTGAAC AGCCGGTGGA TAGAGTTCTG CCAATTGCTA 
AGCGAGAGAC TTAACTGGCT GGAGTATCAG AACAACATCA TCACTTTCTA TAATCAGCTA 
CAACAATTGG AGCAGATGAC AACTACTGCT GAAAACTGGT TGAAAACCCA GCCTACCACC 
ACATCAGAGC CAACAGCAAT TAAAAGCCAG TTAAAAATTT GTAAGGATGA AATCAACCGA 
CTGTCAGCTC TTCAGCCTCA AATCGAGCGA TTAAAAATTC AAAGCATAGC CCTGAAAGAG 
AAAGGACAAG GGCCAATGTT CCTGGATGCA GACTTTGTGG CCTTTACAAA TCATTTTAAC 
CAAGTCTTTG CTGATGTGCA GGCAAGAGAA AAAGAGCTAC AAACAATTTT TGACAGTTTG 
CCACCCATGC GCTATCAGGA GACTATGAGT ACCATCCTGA CATGGATCCA GCAGTCAGAA 
ACCAAACTCT CTATACCTCA GGTTACTGTC ACTGAATATG ACATCATGGA ACAGAGACTC 
GGAGAGCTAC AGGCTTTACA AAGCTCTCTG CAAGAGCAAC AAAATGGCCT AAACTATCTC 
AGCACCACTG TGAAAGAGAT GTCAAAGAAA GCACCACTGT CTGATATTAG TCGGAAATAT 
CAATCAGAAT TTGAAGAGAT TGAGGGACGT TGGAAGAAGC TGTCTTCCCA GCTGGTTGAA 
CATTGTCAAA AGTTGGAGGA GCAAATGGCT AAACTTCGAA AAATTCAGAA TCACATAAAA 
ACTCTGAAGA AATGGATCAC TGAAGTCGAT GTTTTCCTGA AGGAGGAATG GCCTGCCCTT 
GGGGATTCAG AAATTCTGAA AAGACAGCTG AAACAGTGCA GGCTTTTAGT CAATGACATT 
CAGACCATCC AGCCTAGTCT CAACAGTGTC AATGAAGGGG CTCAGAAGAT GAAGAATGAA 
GCAGAACCAG AGTTTGCTGG CAGACTTGAG ACAGAGCTCC GAGAACTTAA CACCCAGTGG 
GATTACATGT GCCGCCAGGT CTATGCCAGG AAGGAAGCCT TAAAAGGAGG TTTGGATAAA 
ACTGTAAGTC TTCAGAAAGA TCTGTCAGAG ATGCATGAGT GGATGACACA AGCTGAAGAA 
GAATACCTAG AGAGAGATTT CGAATACAAG ACCCCTGATG AATTACAGAC AGCAGTTGAA 
GAGATGAAGA GAGCTAAAGA AGAGGCCCAG CAAAAAGAAG CAAAAGTGAA ACTCCTAACC 
GAGTCCGTCA ATAGTGTCAT AGCTCAGGCT CCACCTGCAG CACAAGAGGC CTTAAAAAAG 
GAACTTGACA CTCTCACCAC CAACTACCAG TGGCTCTGCA CCAGGCTCAA TGGCAAATGC 
AAGACCTTGG AAGAAGTTTG GGCGTGCTGG CATGAGTTAT TGTCCTACTT GGAGAAGGCA 
AACAAGTGGC TAAGTGAAGT AGAAGTCAAG CTTAAAACCA CTGAAAATAT TTCTGGGGGA 
GCTGAGGAAA TCGCCGAGGT GCTTGATTCG CTTGAAAATT TGATGCAACA TTCAGAGGAT 
AACCCGAATC AGATTCGCAT ATTGGCACAG ACCTTGACAG ATGGTGGAGT CATGGATGAA 
CTGATCAATG AGGAGCTTGA GACATTTAAT TCTCGTTGGA GAGAACTCCA TGAAGAGGCT 
GTGAGGAGGC AAAAGTTGCT TGAGCAGAGT ATCCAGTCGG CCCAGGAGAT AGAAAAATCC 
TTGCACTTAA TTCAGGAGTC CCTCTCTTCC ATTGACAAGC AGTTGGCAGC TTATATTGCT 
GACAAAGTGG ATGCAGCTCA GATGCCTCAG GAAGCCCAGA AAATCCAATC AGATTTGACA 
AGTCATGAGA TCAGTTTAGA AGAAATGAAG AAACATAACC AGGGAAAGGA GACTGCCCAA 
AGGGTACTAT CCCAAATTGA TGTGGCACAG AAAAAATTGC AGGATGTTTC CATGAAGTTT 
CGATTATTCC AGAAGCCAGC CAATTTTGAG CAGCGCCTAC AAGAAAGTAA AATGATTTTA 
GATGAAGTGA AGATGCATTT ACCTGCGTTG GAAACAAAGA GTGTGGAACA GGAAGTAGTA 
CAGTCACAGT TAAATCATTG TGTGAACTTG TATAAAAGTC TGAGTGAAGT GAAGTCTGAA 
GTGGAAATGG TAATAAAAAC TGGACGTCAG ATTGTACAGA AGAAGCAGAC GGAAAACCCG 
AAAGAGCTTG ATGAAAGAGT TACAGCTTTG AAATTGCATT ATAATGAGCT GGGAGCAAAG 
GTGACAGAAA GAAAGCAACA GTTGGAAAAA TGCTTGAAAT TGTCCCGTAA GATGCGAAAG 
GAAATGAATG CCCTGACAGA ATGGCTGGCA GCGACAGATA TGGAACTGAC GAAGAGATCG 
GCAGTTGAAG GAATGCCTAG TAATTTGGAT TCTGAAGTTG CCTGGGGAAA GGCTACTCAG 
AAAGAGATTG AGAAACAGAA GGTTCACCTA AAGAGTGTCA CAGAGGTAGG AGAGGCCTTG 
AAAACGGTTT TGGGCAAGAA GGAAATGTTG GTGGAAGATA AACTGAGTCT TCTGAATAGT 
AACTGGATAG CCGTCACTTC CCGAGCAGAA GAGTGGTTAA ACCTTTTATT GGAATACCAG 
AAACACATGG AAAACTTTGA CCAGAATGTG GATTACATCA CAAACTGGAT CATTCAGGCT 
GATGCACTTT TGGATGAATC TGAGAAAAAG AAACCTCAGC AAAAAGAAGA CATACTTAAG 
CGTTTAAAGG CTGAAATGAA TGACATACGT CCAAAGGTGG ATTCTACACG TGACCAAGCA 
GCAAACCTGA TGGCAAACCG CGGCGACCAC TGCAGGAAAG TAGTAGAGCC CAAAATCTCA 
GAGCTCAACC ATCGATTTGC AGCCATTTCT CACAGAATTA AGACTGGAAA GGCCTCCATT 
CCTTTGAAGG AATTGGAGCA GTTTAACTCA GATATACAAA AATTGCTTGA ACCACTGGAG 
GCTGAAATTC AGCAGGGGGT GAATCTGAAA GAGGAAGACT TCAATAAAGA TATGAGTGAA 
GACAATGAGG GTACTGTAAA AGAATTGTTG CAAAGAGGAG ACAACTTACA ACAAAGAATC 
ACAGATGAGA GAAAGCGAGA GGAAATAAAG ATAAAACAAC AGCTGTTACA GACAAAACAT 
AATGCTCTCA AGGATTTGAG GTCTCAAAGA AGAAAAAAGG CTCTAGAAAT TTCTCACCAG 
TGGTATCAGT ACAAGAGGCA GGCTGATGAT CTCCTGAAAT GCTTGGATGA CATTGAAAAA 
AAATTAGCCA GCCTACCTGA ACCCAGAGAT GAAAGGAAAA TAAAGGAAAT TGATCGTGAA 
TTGCAGAAGA AGAAAGAGGA GCTGAATGCA GTGCGTAGGC AAGCTGAGGG CTTGTCTGAG 
GATGGGGCCG CAATGGCAGT GGAGCCAACT CAGATCCAGC TCAGCAAGCG CTGGCGGGAA 
ATTGAGAGCA AATTTGCTCA GTTTCGAAGA CTCAACTTTG CACAAATTCA CACTGTCCAT 
GAAGAGTCAG TGGTGGCGAT GACTGAAGAC ATGCCTTTGG AAATTTCTTA TGTGCCTTCT 
ACTTACCTGA CTGAGATCAC TCATGTCTCA CAAGCCCTAT CAGAAGTGGA AGAGCTTCTT 
AATGCTCCCG ACCTTTGTGC TCAAGATTTT GAAGATCTCT TTAAACAAGA GGAATCCTTG 
AAGAACATAA AAGACAGCCT GCAACAAATC TCAGGTCGGA TTGACATCAT TCACAATAAA 
AAGACAGCAG CATTGCACAG TGCCACTCCT GCAGAAAGGG CAAAGCTCCA GGAAGCTCTC 
TCACGGCTTG ATTTCCAATG GGAAAGAGTT AACAATATGT ACAAGGACCG ACAAGGGAGA 
TTTGACAGAT CTGTGGAAAA ATGGCGGCGG TTTCATTATG ATATGAAGAT ACTTAATCAA 
TGGCTAACAG AAGCTGAACA GTTTCTCAAA AAGACACAAA TTCCTGAGAA TTGGGAACAT 
GCCAAATACA AATGGTATCT TAAGGAACTC CAGGATGGCA TTGGACAGCG GCAAAGTGTT 
GTCAGGGTAT TGAATGCAAC TGGGGAAGAA ATAATTCAAC AGTCCTCAAA AACAGATGCC 
AGTATTCTCC AAGAAAAACT GGGAAGCCTG AATCTGCGGT GGCAGGAGGT CTGCAAACAG 
CTGGCAGAAA GAAAAAAGAG GCTAGAGGAA CAGAAGAATA TCTTGTCAGA ATTTCAAAGA 
GATGTAAATG AATTTGTTTT ATGGTTGGAA GAAGCGGATA ACGTTGCTAA TATTCCACTT 
GAACCTGGAA ATGAGCAGCA GCTAAAAGAA AAACTTGAAC AAGTCAAGTT ACTGGCAGAA 
GAGTTGCCCC TGCGCCAGGG AATTCTAAAA CAATTAAATG AAACTGGAGG AACAGTGCTT 
GTAAGTGCTC CCCTAAGCCC AGAAGAGCAA GATAAACTTG AAAATAAGCT CAAGCAGACA 
AATCTTCAGT GGATAAAGGT TTCTAGAAAT CTGCCTGAGA AGCAAGAAGA AATTGAGGCA 
CACGTAAAAG ACCTTGGACA GCTGGAAGAG CAGTTAAATC ATCTGCTTCT ATGGCTGTCT 
CCTATTAGGA ATCAGTTGGA AATTTACAAT CAGCCAAATC AAACAGGACC ATTTGACATC 
AAGGAAATTG AAGTAGCAGT TCAAGCTAAA CAGCCGGATG TGGAAGGGAT TTTGTCTAAA 
GGGCAGCATT TGTACAAGGA AAAACCAGCC ACTCAGCCAG CGAAGAGAAA GCTGGAAGAT 
CTCAGCTCTG ATTGGAAGGT GGTAACTCAG TTGCTTCAAG AGCTGCGGGC AAAGCAGCCT 
GGCCCAGCTC CTGGACTGAC CACTGTCAGA GCCCCTCCCA GTCAGACTGT TACTCTGGTG 
ACACAACCCG CGGTTACCAA GGAAACTGCC ATCTCCAAAC TAGAAATGCC ATCTTCATTG 
CTGTTGGAGG TACCTGCACT GGCAGATTTC AACCGAGCTT GGACAGAACT TACCGACTGG 
CTGTCTCTGC TTGATCGAGT TATAAAATCA CAGAGGGTGA TGGTGGGTGA TCTTGAAGAC 
ATTAACGAGA TGATCATCAA GCAGAAGGCA ACGCTGCAGG ATTTGGAACA GAGGCGCCCC 
CAGTTGGAAG AACTCATTAC CGCTGCCCAG AATTTGAAAA ACAAGACCAG CAATCAAGAG 
GCTAGAACAA TCATTACTGA TCGAATTGAA AGAATTCAGA GTCAGTGGGA TGAAGTACAG 
GAACATCTTC AGAACCGGAG GCAACAGTTG AATGAAATGT TAAAGGATTC CACACAATGG 
CTGGAAGCTA AAGAGGAGGC TGAGCAGGTG TTGGGGCAGG CCAGAGCCAA GCTTGAGTCA 
TGGAAGGAGG CTCCCTACAC AGTAGATGCA ATCCAAAAGA AAATCACAGA AACCAAGCAG 
TTGGCCAAAG ACCTCCGCCA GTGGCAGATA AATGTAGATG TTGCAAATGA TTTGGCACTG 
AAACTTCTCC GAGATTATTC TGCAGATGAT ACCAGAAAAG TACACATGAT AACAGAGAAC 
ATCAATGCCT CTTGGGCAAG CATCCATAAA AGATTGAGTG AGCGAGAGGC TGCTCTGGAA 
GAAACCCACA GATTACTGCA ACAGTTCCCC TTGGACCTGG AGAAGTTCCT TGCCTGGCTT 
ACAGAAGCCG AAACAACTGC CAACGTCCTG CAGGATGCCA CCCATAAGGA AAGGCTTCTA 
GAAGATTCCA AGGGAGTAAG AGAGCTGATG AAACAATGGC AAGATCTCCA AGGAGAAATC 
GAAGCTCACA CAGATATCTA TCACAACCTG GACGAAAATG GCCAAAAAGT CCTGAGATCC 
CTGGAAGGTT CTGACGATGC AGCCTTGTTG CAAAGACGTT TGGATAACAT GAACTTCAAG 
TGGAGCGAAC TTCGGAAAAA GTCTCTCAAC ATTAGGTCTC ACTTGGAAGC CAGTTCTGAC 
CAGTGGAAGC GTCTGCACCT TTCTCTTCAG GAACTTCTGG TATGGCTCCA GCTGAAAGAT 
GATGAGTTAA GCCGGCAGGC ACCCATTGGA GGAGACTTTC CAGCGGTGCA GAAGCAGAAT 
GATGTACACA GGGCCTTCAA GAGGGAATTG AAAACGAAAG AACCTGTAAT CATGAGTACT 
CTTGAGACTG TACGAATATT TCTGACAGAG CAGCCTTTAG AAGGACTAGA GAAACTCTAC 
CAGGAGCCCA GAGAGCTGCC TCCTGAAGAG AGAGCCCAGA ATGTCACACG GCTCCTACGA 
AAGCAAGCTG AGGAGGTCAA CACTCAGTGG GAAAAACTGA ACGTGCACTC TGCAGACTGG 
CAGAGAAAAA TAGACGAGGC CCTCGAAAGA CTCCAGGAGC TTCAGGAAGC AACAGATGAG 
CTGGATCTCA AACTACGTCA GGCAGAGGTG ATCAAAGGAT CCTGGCAGCC TGTGGGTGAT 
CTCCTCATTG ACTCTCTCCA AGATCACCTC GAAAAAGTCA AGGCGCTTCG AGGAGAAATT 
ACACCTCTGA AAGAGAATGT CAGCTACGTC AATGACCTTG CTCGCCAACT CACTACGTTG 
GGCATTCAGC TGTCACCATA TAACCTCAAC ACTCTGGAAG ACCTGAACAC CAGATGGAAG 
CTTCTGCAGG TGGCCATTGA GGACCGCATC AGGCAGCTGC ATGAAGCGCA CAGGGACTTT 
GGACCAGCCT CCCAGCACTT CCTTTCCACT TCTGTCCAGG GTCCCTGGGA GAGAGCCATC 
TCACCAAACA AAGTGCCCTA CTATATCAAC CACGAGACCC AAACAACTTG CTGGGACCAT 
CCCAAAATGA CAGAGCTCTA CCAGTCTTTA GCTGACCTGA ATAATGTCAG GTTCTCAGCT 
TACAGGACTG CCATGAAACT CCGAAGACTG CAGAAGGCCC TTTGCTTGGA TCTCTTGAGC 
CTATCGGCTG CATGCGATGC CTTGGACCAG CACAACCTCA AGCAAAATGA CCAGCCCATG 
GATATCCTGC AGGTCATTAA CTGTCTGACC ACTATTTATG ATCGCCTAGA GCAAGAGCAC 
AACAATCTGG TCAACGTCCC TCTCTGCGTG GATATGTGTC TCAATTGGCT GCTGAATGTT 
TATGACACGG GACGAACGGG GAGGATCCGG GTCCTGTCTT TTAAAACTGG CATCATTTCT 
CTGTGTAAAG CCCATTTGGA AGACAAGTAC AGATACCTCT TCAAGCAAGT GGCAAGTTCG 
ACAGGATTTT GTGACCAGCG CAGGCTGGGC CTCCTCCTGC ATGACTCTAT CCAGATCCCA 
AGACAGTTGG GTGAAGTCGC ATCGTTCGGG GGCAGTAACA TTGAGCCGAG TGTCAGGAGC 
TGCTTCCAGT TTGCTAATAA TAAGCCTGAG ATCGAAGCGG CCCTCTTCCT AGACTGGATG 
CGCCTGGAGC CCCAGTCCAT GGTGTGGCTG CCTGTCCTGC ACCGAGTGGC TGCCGCGGAA 
ACTGCCAAGC ACCAGGCCAA GTGCAACATC TGCAAGGAGT GTCCCATCAT CGGATTCAGG 
TACAGGAGTC TAAAGCACTT TAATTATGAC ATCTGCCAAA GTTGCTTTTT TTCTGGTCGA 
GTTGCAAAAG GCCATAAAAT GCACTATCCC ATGGTGGAAT ACTGCACTCC GACTACATCG 
GGAGAAGATG TCCGTGACTT TGCCAAGGTA CTAAAAAACA AATTTCGAAC CAAAAGGTAT 
TTTGCGAAGC ATCCCCGAAT GGGCTACCTG CCAGTGCAGA CTGTCTTAGA GGGGGACAAC 
ATGGAAACTC CTGTCACTCT GATCAACTTC TGGCCGGTAG ATTCTGCGCC TGCCTCGTCC 
CCTCAGCTTT CACACGATGA TACTCATTCA CGCATTGAGC ATTATGCTAG CAGGCTAGCA 
GAAATGGAAA ACAGCAATGG ATCTTATCTA AATGATAGCA TCTCTCCTAA TGAGAGCATA 
GATGATGAAC ATTTGTTAAT CCAGCATTAC TGCCAAAGTT TGAACCAGGA CTCCCCCCTG 
AGCCAGCCTC GTAGTCCTGC CCAGATCTTG ATTTCCTTAG AGAGTGAGGA AAGAGGGGAG 
CTAGAGAGAA TCCTAGCAGA TCTTGAGGAA GAAAACAGAA ATCTGCAAGC AGAGTATGAT 
CGTCTAAAGC AGCAGCATGA ACACAAAGGC CTGTCCCCAC TGCCATCCCC TCCTGAAATG 
ATGCCTACTT CTCCCCAAAG TCCCCGGGAT GCTGAGCTCA TCGCTGAGGC CAAGCTGCTG 
CGTCAACACA AAGGCCGCCT GGAAGCCAGG ATGCAAATCC TTGAAGACCA TAACAAACAA 
CTGGAATCCC AGTTACACAG GCTCAGGCAG CTGCTGGAGC AACCCCAGGC AGAGGCCAAG 
GTGAATGGTA CAACGGTGTC TTCTCCTTCT ACCTCTCTTC AGAGGTCAGA TAGCAGTCAG 
CCTATGCTGC TCCGGGTGGT CGGCAGTCAG ACTTCAGAAT CCATGGGCGA GGAAGACCTG 
CTCAGCCCTC CCCAGGACAC AAGCACAGGG TTAGAGGAAG TGATGGAGCA GCTCAACCAC 
TCCTTCCCTA GTTCCAGAGG ACACAATGTA GGAAGCCTTT TCCACTTGGC AGATGATTTG 
GGCAGAGCGA TGGAGTCCTT AGTTTCAGTC ATGACAGATG AAGAAGGGGC AGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMD ( XM_005641030.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005641087.1 Canis lupus familiaris dystrophin (DMD), transcript variant X5, mRNA.

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CloneID	OCe31974
Clone ID Related Accession (Same CDS sequence)	XM_005641030.3 , XM_005641030.2
Accession Version	XM_005641030.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	dystrophin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005641030.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTGGT GGGAAGAAGT AGAGGACTGT TATGAAAGAG AAGATGTTCA AAAGAAAACA 
TTCACAAAAT GGATAAATGC ACAGTTTTCT AAGTTTGGGA AGCAGCACAT AGAGAACCTC 
TTCAGTGACC TACAGGATGG GAGACGCCTC CTAGACCTTT TGGAAGGCCT GACAGGGCAA 
AAACTGCCAA AAGAAAAAGG ATCCACAAGA GTTCATGCCC TGAACAATGT CAACAAGGCA 
CTGCGCGTCT TGCAGAAAAA TAATGTTGAT TTAGTGAACA TTGGAAGTAC TGACATAGTA 
GATGGAAATC ACAAACTGAC TCTTGGTTTG ATTTGGAATA TAATCCTCCA CTGGCAGGTC 
AAAAATGTAA TGAAAAATAT CATGGCTGGA TTGCAACAAA CCAACAGTGA AAAGATTCTC 
CTGAGCTGGG TCCGACAATC AACTCGTAAT TATCCACAGG TTAATGTCAT TAACTTCACC 
ACCAGCTGGT CTGATGGCCT GGCTTTGAAC GCTCTCATCC ACAGTCATAG GCCAGACCTG 
TTTGATTGGA ATAGTGTGGT TTGCCAGCAG TCAGCCACAC AACGCCTGGA ACATGCATTC 
AACATTGCCA AATATCAATT AGGCATAGAG AAACTGCTTG ATCCTGAAGA TGTTGCCACC 
ACTTATCCAG ATAAGAAGTC CATCTTAATG TATATCACAT CACTCTTCCA AGTTTTGCCT 
CAACAAGTGA GCATTGAAGC CATCCAGGAA GTGGAAATGT TGCCAAGGCC ATCAAAAGTT 
ACTAGAGAAG AACATTTTCA ATTACATCAT CAAATGCACT ATTCTCAACA GATCACAGTC 
AGTCTAGCAC AGGGCTATGA ACGAGCCCCT TCCTCTCCTA AGCCTCGGTT CAAGAGCTAT 
GCCTACACAC AGGCTGCTTA TGTCACCACT TCTGACCCCA CACGGAGCCC ACTTCCTTCA 
CAGCATTTGG AAACTCCTGA AGACAAGTCA TTTGGCCGGT CATTGACAGA GACCGAAGCA 
AACCTGGACA GTTATCAAAC AGCTTTGGAA GAAGTACTCT CGTGGCTTCT TTCAGCTGAG 
GATGCACTGC AAGCCCAAGG AGAGATTTCT AATGATGTCG AAGAAGTGAA AGAACAATTT 
CATACTCATG AGGGATATAT GATGGACTTG ACATCCCATC AGGGACGGGT CGGTAATGTT 
CTCCAACTGG GAAGTCAACT GATTGGAACA GGGAAATTAT CAGAAGATGA AGAAACCGAA 
GTGCAGGAAC AAATGAATCT CCTCAATTCA AGATGGGAAT GCCTCAGGGT AGCTAGCATG 
GAAAAACAAA GCAATTTACA TAAAGTTCTA ATGGATCTCC AGAATCAGCA ACTGAAAGAG 
TTAAATGACT GGCTAACCAA AACAGAAGAG AGAACAAGGA AAATGGAGAA GGAGCCCCTT 
GGACCTGATA TTGAAGACCT AAAACGCCAA GTACAACAAC ATAAGGTGCT TCAAGAAGAC 
TTAGAACAGG AACAAGTCAG GGTCAATTCC CTCACTCATA TGGTGGTGGT AGTCGATGAA 
TCTAGTGGAG ACCATGCAAC TGCTGCTTTG GAAGAACAAC TTAAGGTACT GGGAGATCGA 
TGGGCAAACA TCTGTAGGTG GACAGAAGAT CGCTGGGTTC TTTTACAAGA CATCCTCCTA 
AAATGGCAGC GTTTTACTGA AGAACAGTGC CTTTTCAGTG CATGGCTTTC GGAGAAGGAA 
GATGCAGTGA ACAAGATTCA CACAACTGGC TTTAAGGATC AAAGTGAAGT GTTATCAAAT 
CTTCAGAAAC TGGCTGTCTT AAAAACAGAT CTGGAAAAGA AGAAGCAAAC CATGGACAAA 
CTCTGCTCAC TCAACCAAGA CCTTCTTTCA GCGCTGAAAA ACACAGTGGT AGCCCACAAG 
ATGGAAGCAT GGCTGGACAA CTTTGCCCAG CGCTGGGATA ATTTAGTCCA GAAACTTGAA 
AAAAGTTCAG CACAGATTTC ACAGGCTGTC ACCACCACTC AGCCATCACT AACACAGACA 
ACTGTAATGG AAACAGTAAC TATGGTGACC ACGAGGGAAC ACATCTTGGT AAAGCATGCC 
CAAGAGGAAC TGCCACCACC ACCCCCTCAG AAGAAGAGGC AGATTATCGT GGATTCTGAA 
ATTAGGAAAA GGTTGGATGT CGATATAACT GAACTTCACA GTTGGATTAC TCGTTCAGAA 
GCTGTGTTGC AGAGTCCTGA ATTTGCAATC TATCGGAAGG AAGGCAACTT CTCAGACCTT 
AAAGAAAAAG TCAATGCCAT AGAGCGAGAA AAAGCCGAGA AGTTCAGAAA ACTGCAAGAT 
GCCAGCAGAT CAGCTCAGGC CCTGGTGGAA CAGATGGTGA ATGAGGGTGT TAATGCTGAC 
AGCATCAAAC AAGCCTCCGA ACAACTGAAC AGCCGGTGGA TAGAGTTCTG CCAATTGCTA 
AGCGAGAGAC TTAACTGGCT GGAGTATCAG AACAACATCA TCACTTTCTA TAATCAGCTA 
CAACAATTGG AGCAGATGAC AACTACTGCT GAAAACTGGT TGAAAACCCA GCCTACCACC 
ACATCAGAGC CAACAGCAAT TAAAAGCCAG TTAAAAATTT GTAAGGATGA AATCAACCGA 
CTGTCAGCTC TTCAGCCTCA AATCGAGCGA TTAAAAATTC AAAGCATAGC CCTGAAAGAG 
AAAGGACAAG GGCCAATGTT CCTGGATGCA GACTTTGTGG CCTTTACAAA TCATTTTAAC 
CAAGTCTTTG CTGATGTGCA GGCAAGAGAA AAAGAGCTAC AAACAATTTT TGACAGTTTG 
CCACCCATGC GCTATCAGGA GACTATGAGT ACCATCCTGA CATGGATCCA GCAGTCAGAA 
ACCAAACTCT CTATACCTCA GGTTACTGTC ACTGAATATG ACATCATGGA ACAGAGACTC 
GGAGAGCTAC AGGCTTTACA AAGCTCTCTG CAAGAGCAAC AAAATGGCCT AAACTATCTC 
AGCACCACTG TGAAAGAGAT GTCAAAGAAA GCACCACTGT CTGATATTAG TCGGAAATAT 
CAATCAGAAT TTGAAGAGAT TGAGGGACGT TGGAAGAAGC TGTCTTCCCA GCTGGTTGAA 
CATTGTCAAA AGTTGGAGGA GCAAATGGCT AAACTTCGAA AAATTCAGAA TCACATAAAA 
ACTCTGAAGA AATGGATCAC TGAAGTCGAT GTTTTCCTGA AGGAGGAATG GCCTGCCCTT 
GGGGATTCAG AAATTCTGAA AAGACAGCTG AAACAGTGCA GGCTTTTAGT CAATGACATT 
CAGACCATCC AGCCTAGTCT CAACAGTGTC AATGAAGGGG CTCAGAAGAT GAAGAATGAA 
GCAGAACCAG AGTTTGCTGG CAGACTTGAG ACAGAGCTCC GAGAACTTAA CACCCAGTGG 
GATTACATGT GCCGCCAGGT CTATGCCAGG AAGGAAGCCT TAAAAGGAGG TTTGGATAAA 
ACTGTAAGTC TTCAGAAAGA TCTGTCAGAG ATGCATGAGT GGATGACACA AGCTGAAGAA 
GAATACCTAG AGAGAGATTT CGAATACAAG ACCCCTGATG AATTACAGAC AGCAGTTGAA 
GAGATGAAGA GAGCTAAAGA AGAGGCCCAG CAAAAAGAAG CAAAAGTGAA ACTCCTAACC 
GAGTCCGTCA ATAGTGTCAT AGCTCAGGCT CCACCTGCAG CACAAGAGGC CTTAAAAAAG 
GAACTTGACA CTCTCACCAC CAACTACCAG TGGCTCTGCA CCAGGCTCAA TGGCAAATGC 
AAGACCTTGG AAGAAGTTTG GGCGTGCTGG CATGAGTTAT TGTCCTACTT GGAGAAGGCA 
AACAAGTGGC TAAGTGAAGT AGAAGTCAAG CTTAAAACCA CTGAAAATAT TTCTGGGGGA 
GCTGAGGAAA TCGCCGAGGT GCTTGATTCG CTTGAAAATT TGATGCAACA TTCAGAGGAT 
AACCCGAATC AGATTCGCAT ATTGGCACAG ACCTTGACAG ATGGTGGAGT CATGGATGAA 
CTGATCAATG AGGAGCTTGA GACATTTAAT TCTCGTTGGA GAGAACTCCA TGAAGAGGCT 
GTGAGGAGGC AAAAGTTGCT TGAGCAGAGT ATCCAGTCGG CCCAGGAGAT AGAAAAATCC 
TTGCACTTAA TTCAGGAGTC CCTCTCTTCC ATTGACAAGC AGTTGGCAGC TTATATTGCT 
GACAAAGTGG ATGCAGCTCA GATGCCTCAG GAAGCCCAGA AAATCCAATC AGATTTGACA 
AGTCATGAGA TCAGTTTAGA AGAAATGAAG AAACATAACC AGGGAAAGGA GACTGCCCAA 
AGGGTACTAT CCCAAATTGA TGTGGCACAG AAAAAATTGC AGGATGTTTC CATGAAGTTT 
CGATTATTCC AGAAGCCAGC CAATTTTGAG CAGCGCCTAC AAGAAAGTAA AATGATTTTA 
GATGAAGTGA AGATGCATTT ACCTGCGTTG GAAACAAAGA GTGTGGAACA GGAAGTAGTA 
CAGTCACAGT TAAATCATTG TGTGAACTTG TATAAAAGTC TGAGTGAAGT GAAGTCTGAA 
GTGGAAATGG TAATAAAAAC TGGACGTCAG ATTGTACAGA AGAAGCAGAC GGAAAACCCG 
AAAGAGCTTG ATGAAAGAGT TACAGCTTTG AAATTGCATT ATAATGAGCT GGGAGCAAAG 
GTGACAGAAA GAAAGCAACA GTTGGAAAAA TGCTTGAAAT TGTCCCGTAA GATGCGAAAG 
GAAATGAATG CCCTGACAGA ATGGCTGGCA GCGACAGATA TGGAACTGAC GAAGAGATCG 
GCAGTTGAAG GAATGCCTAG TAATTTGGAT TCTGAAGTTG CCTGGGGAAA GGCTACTCAG 
AAAGAGATTG AGAAACAGAA GGTTCACCTA AAGAGTGTCA CAGAGGTAGG AGAGGCCTTG 
AAAACGGTTT TGGGCAAGAA GGAAATGTTG GTGGAAGATA AACTGAGTCT TCTGAATAGT 
AACTGGATAG CCGTCACTTC CCGAGCAGAA GAGTGGTTAA ACCTTTTATT GGAATACCAG 
AAACACATGG AAAACTTTGA CCAGAATGTG GATTACATCA CAAACTGGAT CATTCAGGCT 
GATGCACTTT TGGATGAATC TGAGAAAAAG AAACCTCAGC AAAAAGAAGA CATACTTAAG 
CGTTTAAAGG CTGAAATGAA TGACATACGT CCAAAGGTGG ATTCTACACG TGACCAAGCA 
GCAAACCTGA TGGCAAACCG CGGCGACCAC TGCAGGAAAG TAGTAGAGCC CAAAATCTCA 
GAGCTCAACC ATCGATTTGC AGCCATTTCT CACAGAATTA AGACTGGAAA GGCCTCCATT 
CCTTTGAAGG AATTGGAGCA GTTTAACTCA GATATACAAA AATTGCTTGA ACCACTGGAG 
GCTGAAATTC AGCAGGGGGT GAATCTGAAA GAGGAAGACT TCAATAAAGA TATGAGTGAA 
GACAATGAGG GTACTGTAAA AGAATTGTTG CAAAGAGGAG ACAACTTACA ACAAAGAATC 
ACAGATGAGA GAAAGCGAGA GGAAATAAAG ATAAAACAAC AGCTGTTACA GACAAAACAT 
AATGCTCTCA AGGATTTGAG GTCTCAAAGA AGAAAAAAGG CTCTAGAAAT TTCTCACCAG 
TGGTATCAGT ACAAGAGGCA GGCTGATGAT CTCCTGAAAT GCTTGGATGA CATTGAAAAA 
AAATTAGCCA GCCTACCTGA ACCCAGAGAT GAAAGGAAAA TAAAGGAAAT TGATCGTGAA 
TTGCAGAAGA AGAAAGAGGA GCTGAATGCA GTGCGTAGGC AAGCTGAGGG CTTGTCTGAG 
GATGGGGCCG CAATGGCAGT GGAGCCAACT CAGATCCAGC TCAGCAAGCG CTGGCGGGAA 
ATTGAGAGCA AATTTGCTCA GTTTCGAAGA CTCAACTTTG CACAAATTCA CACTGTCCAT 
GAAGAGTCAG TGGTGGCGAT GACTGAAGAC ATGCCTTTGG AAATTTCTTA TGTGCCTTCT 
ACTTACCTGA CTGAGATCAC TCATGTCTCA CAAGCCCTAT CAGAAGTGGA AGAGCTTCTT 
AATGCTCCCG ACCTTTGTGC TCAAGATTTT GAAGATCTCT TTAAACAAGA GGAATCCTTG 
AAGAACATAA AAGACAGCCT GCAACAAATC TCAGGTCGGA TTGACATCAT TCACAATAAA 
AAGACAGCAG CATTGCACAG TGCCACTCCT GCAGAAAGGG CAAAGCTCCA GGAAGCTCTC 
TCACGGCTTG ATTTCCAATG GGAAAGAGTT AACAATATGT ACAAGGACCG ACAAGGGAGA 
TTTGACAGAT CTGTGGAAAA ATGGCGGCGG TTTCATTATG ATATGAAGAT ACTTAATCAA 
TGGCTAACAG AAGCTGAACA GTTTCTCAAA AAGACACAAA TTCCTGAGAA TTGGGAACAT 
GCCAAATACA AATGGTATCT TAAGGAACTC CAGGATGGCA TTGGACAGCG GCAAAGTGTT 
GTCAGGGTAT TGAATGCAAC TGGGGAAGAA ATAATTCAAC AGTCCTCAAA AACAGATGCC 
AGTATTCTCC AAGAAAAACT GGGAAGCCTG AATCTGCGGT GGCAGGAGGT CTGCAAACAG 
CTGGCAGAAA GAAAAAAGAG GCTAGAGGAA CAGAAGAATA TCTTGTCAGA ATTTCAAAGA 
GATGTAAATG AATTTGTTTT ATGGTTGGAA GAAGCGGATA ACGTTGCTAA TATTCCACTT 
GAACCTGGAA ATGAGCAGCA GCTAAAAGAA AAACTTGAAC AAGTCAAGTT ACTGGCAGAA 
GAGTTGCCCC TGCGCCAGGG AATTCTAAAA CAATTAAATG AAACTGGAGG AACAGTGCTT 
GTAAGTGCTC CCCTAAGCCC AGAAGAGCAA GATAAACTTG AAAATAAGCT CAAGCAGACA 
AATCTTCAGT GGATAAAGGT TTCTAGAAAT CTGCCTGAGA AGCAAGAAGA AATTGAGGCA 
CACGTAAAAG ACCTTGGACA GCTGGAAGAG CAGTTAAATC ATCTGCTTCT ATGGCTGTCT 
CCTATTAGGA ATCAGTTGGA AATTTACAAT CAGCCAAATC AAACAGGACC ATTTGACATC 
AAGGAAATTG AAGTAGCAGT TCAAGCTAAA CAGCCGGATG TGGAAGGGAT TTTGTCTAAA 
GGGCAGCATT TGTACAAGGA AAAACCAGCC ACTCAGCCAG CGAAGAGAAA GCTGGAAGAT 
CTCAGCTCTG ATTGGAAGGT GGTAACTCAG TTGCTTCAAG AGCTGCGGGC AAAGCAGCCT 
GGCCCAGCTC CTGGACTGAC CACTGTCAGA GCCCCTCCCA GTCAGACTGT TACTCTGGTG 
ACACAACCCG CGGTTACCAA GGAAACTGCC ATCTCCAAAC TAGAAATGCC ATCTTCATTG 
CTGTTGGAGG TACCTGCACT GGCAGATTTC AACCGAGCTT GGACAGAACT TACCGACTGG 
CTGTCTCTGC TTGATCGAGT TATAAAATCA CAGAGGGTGA TGGTGGGTGA TCTTGAAGAC 
ATTAACGAGA TGATCATCAA GCAGAAGGCA ACGCTGCAGG ATTTGGAACA GAGGCGCCCC 
CAGTTGGAAG AACTCATTAC CGCTGCCCAG AATTTGAAAA ACAAGACCAG CAATCAAGAG 
GCTAGAACAA TCATTACTGA TCGAATTGAA AGAATTCAGA GTCAGTGGGA TGAAGTACAG 
GAACATCTTC AGAACCGGAG GCAACAGTTG AATGAAATGT TAAAGGATTC CACACAATGG 
CTGGAAGCTA AAGAGGAGGC TGAGCAGGTG TTGGGGCAGG CCAGAGCCAA GCTTGAGTCA 
TGGAAGGAGG CTCCCTACAC AGTAGATGCA ATCCAAAAGA AAATCACAGA AACCAAGCAG 
TTGGCCAAAG ACCTCCGCCA GTGGCAGATA AATGTAGATG TTGCAAATGA TTTGGCACTG 
AAACTTCTCC GAGATTATTC TGCAGATGAT ACCAGAAAAG TACACATGAT AACAGAGAAC 
ATCAATGCCT CTTGGGCAAG CATCCATAAA AGATTGAGTG AGCGAGAGGC TGCTCTGGAA 
GAAACCCACA GATTACTGCA ACAGTTCCCC TTGGACCTGG AGAAGTTCCT TGCCTGGCTT 
ACAGAAGCCG AAACAACTGC CAACGTCCTG CAGGATGCCA CCCATAAGGA AAGGCTTCTA 
GAAGATTCCA AGGGAGTAAG AGAGCTGATG AAACAATGGC AAGATCTCCA AGGAGAAATC 
GAAGCTCACA CAGATATCTA TCACAACCTG GACGAAAATG GCCAAAAAGT CCTGAGATCC 
CTGGAAGGTT CTGACGATGC AGCCTTGTTG CAAAGACGTT TGGATAACAT GAACTTCAAG 
TGGAGCGAAC TTCGGAAAAA GTCTCTCAAC ATTAGGTCTC ACTTGGAAGC CAGTTCTGAC 
CAGTGGAAGC GTCTGCACCT TTCTCTTCAG GAACTTCTGG TATGGCTCCA GCTGAAAGAT 
GATGAGTTAA GCCGGCAGGC ACCCATTGGA GGAGACTTTC CAGCGGTGCA GAAGCAGAAT 
GATGTACACA GGGCCTTCAA GAGGGAATTG AAAACGAAAG AACCTGTAAT CATGAGTACT 
CTTGAGACTG TACGAATATT TCTGACAGAG CAGCCTTTAG AAGGACTAGA GAAACTCTAC 
CAGGAGCCCA GAGAGCTGCC TCCTGAAGAG AGAGCCCAGA ATGTCACACG GCTCCTACGA 
AAGCAAGCTG AGGAGGTCAA CACTCAGTGG GAAAAACTGA ACGTGCACTC TGCAGACTGG 
CAGAGAAAAA TAGACGAGGC CCTCGAAAGA CTCCAGGAGC TTCAGGAAGC AACAGATGAG 
CTGGATCTCA AACTACGTCA GGCAGAGGTG ATCAAAGGAT CCTGGCAGCC TGTGGGTGAT 
CTCCTCATTG ACTCTCTCCA AGATCACCTC GAAAAAGTCA AGGCGCTTCG AGGAGAAATT 
ACACCTCTGA AAGAGAATGT CAGCTACGTC AATGACCTTG CTCGCCAACT CACTACGTTG 
GGCATTCAGC TGTCACCATA TAACCTCAAC ACTCTGGAAG ACCTGAACAC CAGATGGAAG 
CTTCTGCAGG TGGCCATTGA GGACCGCATC AGGCAGCTGC ATGAAGCGCA CAGGGACTTT 
GGACCAGCCT CCCAGCACTT CCTTTCCACT TCTGTCCAGG GTCCCTGGGA GAGAGCCATC 
TCACCAAACA AAGTGCCCTA CTATATCAAC CACGAGACCC AAACAACTTG CTGGGACCAT 
CCCAAAATGA CAGAGCTCTA CCAGTCTTTA GCTGACCTGA ATAATGTCAG GTTCTCAGCT 
TACAGGACTG CCATGAAACT CCGAAGACTG CAGAAGGCCC TTTGCTTGGA TCTCTTGAGC 
CTATCGGCTG CATGCGATGC CTTGGACCAG CACAACCTCA AGCAAAATGA CCAGCCCATG 
GATATCCTGC AGGTCATTAA CTGTCTGACC ACTATTTATG ATCGCCTAGA GCAAGAGCAC 
AACAATCTGG TCAACGTCCC TCTCTGCGTG GATATGTGTC TCAATTGGCT GCTGAATGTT 
TATGACACGG GACGAACGGG GAGGATCCGG GTCCTGTCTT TTAAAACTGG CATCATTTCT 
CTGTGTAAAG CCCATTTGGA AGACAAGTAC AGATACCTCT TCAAGCAAGT GGCAAGTTCG 
ACAGGATTTT GTGACCAGCG CAGGCTGGGC CTCCTCCTGC ATGACTCTAT CCAGATCCCA 
AGACAGTTGG GTGAAGTCGC ATCGTTCGGG GGCAGTAACA TTGAGCCGAG TGTCAGGAGC 
TGCTTCCAGT TTGCTAATAA TAAGCCTGAG ATCGAAGCGG CCCTCTTCCT AGACTGGATG 
CGCCTGGAGC CCCAGTCCAT GGTGTGGCTG CCTGTCCTGC ACCGAGTGGC TGCCGCGGAA 
ACTGCCAAGC ACCAGGCCAA GTGCAACATC TGCAAGGAGT GTCCCATCAT CGGATTCAGG 
TACAGGAGTC TAAAGCACTT TAATTATGAC ATCTGCCAAA GTTGCTTTTT TTCTGGTCGA 
GTTGCAAAAG GCCATAAAAT GCACTATCCC ATGGTGGAAT ACTGCACTCC GACTACATCG 
GGAGAAGATG TCCGTGACTT TGCCAAGGTA CTAAAAAACA AATTTCGAAC CAAAAGGTAT 
TTTGCGAAGC ATCCCCGAAT GGGCTACCTG CCAGTGCAGA CTGTCTTAGA GGGGGACAAC 
ATGGAAACGC CTGCCTCGTC CCCTCAGCTT TCACACGATG ATACTCATTC ACGCATTGAG 
CATTATGCTA GCAGGCTAGC AGAAATGGAA AACAGCAATG GATCTTATCT AAATGATAGC 
ATCTCTCCTA ATGAGAGCAT AGATGATGAA CATTTGTTAA TCCAGCATTA CTGCCAAAGT 
TTGAACCAGG ACTCCCCCCT GAGCCAGCCT CGTAGTCCTG CCCAGATCTT GATTTCCTTA 
GAGAGTGAGG AAAGAGGGGA GCTAGAGAGA ATCCTAGCAG ATCTTGAGGA AGAAAACAGA 
AATCTGCAAG CAGAGTATGA TCGTCTAAAG CAGCAGCATG AACACAAAGG CCTGTCCCCA 
CTGCCATCCC CTCCTGAAAT GATGCCTACT TCTCCCCAAA GTCCCCGGGA TGCTGAGCTC 
ATCGCTGAGG CCAAGCTGCT GCGTCAACAC AAAGGCCGCC TGGAAGCCAG GATGCAAATC 
CTTGAAGACC ATAACAAACA ACTGGAATCC CAGTTACACA GGCTCAGGCA GCTGCTGGAG 
CAACCCCAGG CAGAGGCCAA GGTGAATGGT ACAACGGTGT CTTCTCCTTC TACCTCTCTT 
CAGAGGTCAG ATAGCAGTCA GCCTATGCTG CTCCGGGTGG TCGGCAGTCA GACTTCAGAA 
TCCATGGGCG AGGAAGACCT GCTCAGCCCT CCCCAGGACA CAAGCACAGG GTTAGAGGAA 
GTGATGGAGC AGCTCAACCA CTCCTTCCCT AGTTCCAGAG GACACAATGT AGGAAGCCTT 
TTCCACTTGG CAGATGATTT GGGCAGAGCG ATGGAGTCCT TAGTTTCAGT CATGACAGAT 
GAAGAAGGGG CAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005641087.1
CDS	247..11304
Translation	MLWWEEVEDCYEREDVQKKTFTKWINAQFSKFGKQHIENLFSDLQDGRRLLDLLEGLTGQKLPKEKGSTRVHALNNVNKALRVLQKNNVDLVNIGSTDIVDGNHKLTLGLIWNIILHWQVKNVMKNIMAGLQQTNSEKILLSWVRQSTRNYPQVNVINFTTSWSDGLALNALIHSHRPDLFDWNSVVCQQSATQRLEHAFNIAKYQLGIEKLLDPEDVATTYPDKKSILMYITSLFQVLPQQVSIEAIQEVEMLPRPSKVTREEHFQLHHQMHYSQQITVSLAQGYERAPSSPKPRFKSYAYTQAAYVTTSDPTRSPLPSQHLETPEDKSFGRSLTETEANLDSYQTALEEVLSWLLSAEDALQAQGEISNDVEEVKEQFHTHEGYMMDLTSHQGRVGNVLQLGSQLIGTGKLSEDEETEVQEQMNLLNSRWECLRVASMEKQSNLHKVLMDLQNQQLKELNDWLTKTEERTRKMEKEPLGPDIEDLKRQVQQHKVLQEDLEQEQVRVNSLTHMVVVVDESSGDHATAALEEQLKVLGDRWANICRWTEDRWVLLQDILLKWQRFTEEQCLFSAWLSEKEDAVNKIHTTGFKDQSEVLSNLQKLAVLKTDLEKKKQTMDKLCSLNQDLLSALKNTVVAHKMEAWLDNFAQRWDNLVQKLEKSSAQISQAVTTTQPSLTQTTVMETVTMVTTREHILVKHAQEELPPPPPQKKRQIIVDSEIRKRLDVDITELHSWITRSEAVLQSPEFAIYRKEGNFSDLKEKVNAIEREKAEKFRKLQDASRSAQALVEQMVNEGVNADSIKQASEQLNSRWIEFCQLLSERLNWLEYQNNIITFYNQLQQLEQMTTTAENWLKTQPTTTSEPTAIKSQLKICKDEINRLSALQPQIERLKIQSIALKEKGQGPMFLDADFVAFTNHFNQVFADVQAREKELQTIFDSLPPMRYQETMSTILTWIQQSETKLSIPQVTVTEYDIMEQRLGELQALQSSLQEQQNGLNYLSTTVKEMSKKAPLSDISRKYQSEFEEIEGRWKKLSSQLVEHCQKLEEQMAKLRKIQNHIKTLKKWITEVDVFLKEEWPALGDSEILKRQLKQCRLLVNDIQTIQPSLNSVNEGAQKMKNEAEPEFAGRLETELRELNTQWDYMCRQVYARKEALKGGLDKTVSLQKDLSEMHEWMTQAEEEYLERDFEYKTPDELQTAVEEMKRAKEEAQQKEAKVKLLTESVNSVIAQAPPAAQEALKKELDTLTTNYQWLCTRLNGKCKTLEEVWACWHELLSYLEKANKWLSEVEVKLKTTENISGGAEEIAEVLDSLENLMQHSEDNPNQIRILAQTLTDGGVMDELINEELETFNSRWRELHEEAVRRQKLLEQSIQSAQEIEKSLHLIQESLSSIDKQLAAYIADKVDAAQMPQEAQKIQSDLTSHEISLEEMKKHNQGKETAQRVLSQIDVAQKKLQDVSMKFRLFQKPANFEQRLQESKMILDEVKMHLPALETKSVEQEVVQSQLNHCVNLYKSLSEVKSEVEMVIKTGRQIVQKKQTENPKELDERVTALKLHYNELGAKVTERKQQLEKCLKLSRKMRKEMNALTEWLAATDMELTKRSAVEGMPSNLDSEVAWGKATQKEIEKQKVHLKSVTEVGEALKTVLGKKEMLVEDKLSLLNSNWIAVTSRAEEWLNLLLEYQKHMENFDQNVDYITNWIIQADALLDESEKKKPQQKEDILKRLKAEMNDIRPKVDSTRDQAANLMANRGDHCRKVVEPKISELNHRFAAISHRIKTGKASIPLKELEQFNSDIQKLLEPLEAEIQQGVNLKEEDFNKDMSEDNEGTVKELLQRGDNLQQRITDERKREEIKIKQQLLQTKHNALKDLRSQRRKKALEISHQWYQYKRQADDLLKCLDDIEKKLASLPEPRDERKIKEIDRELQKKKEELNAVRRQAEGLSEDGAAMAVEPTQIQLSKRWREIESKFAQFRRLNFAQIHTVHEESVVAMTEDMPLEISYVPSTYLTEITHVSQALSEVEELLNAPDLCAQDFEDLFKQEESLKNIKDSLQQISGRIDIIHNKKTAALHSATPAERAKLQEALSRLDFQWERVNNMYKDRQGRFDRSVEKWRRFHYDMKILNQWLTEAEQFLKKTQIPENWEHAKYKWYLKELQDGIGQRQSVVRVLNATGEEIIQQSSKTDASILQEKLGSLNLRWQEVCKQLAERKKRLEEQKNILSEFQRDVNEFVLWLEEADNVANIPLEPGNEQQLKEKLEQVKLLAEELPLRQGILKQLNETGGTVLVSAPLSPEEQDKLENKLKQTNLQWIKVSRNLPEKQEEIEAHVKDLGQLEEQLNHLLLWLSPIRNQLEIYNQPNQTGPFDIKEIEVAVQAKQPDVEGILSKGQHLYKEKPATQPAKRKLEDLSSDWKVVTQLLQELRAKQPGPAPGLTTVRAPPSQTVTLVTQPAVTKETAISKLEMPSSLLLEVPALADFNRAWTELTDWLSLLDRVIKSQRVMVGDLEDINEMIIKQKATLQDLEQRRPQLEELITAAQNLKNKTSNQEARTIITDRIERIQSQWDEVQEHLQNRRQQLNEMLKDSTQWLEAKEEAEQVLGQARAKLESWKEAPYTVDAIQKKITETKQLAKDLRQWQINVDVANDLALKLLRDYSADDTRKVHMITENINASWASIHKRLSEREAALEETHRLLQQFPLDLEKFLAWLTEAETTANVLQDATHKERLLEDSKGVRELMKQWQDLQGEIEAHTDIYHNLDENGQKVLRSLEGSDDAALLQRRLDNMNFKWSELRKKSLNIRSHLEASSDQWKRLHLSLQELLVWLQLKDDELSRQAPIGGDFPAVQKQNDVHRAFKRELKTKEPVIMSTLETVRIFLTEQPLEGLEKLYQEPRELPPEERAQNVTRLLRKQAEEVNTQWEKLNVHSADWQRKIDEALERLQELQEATDELDLKLRQAEVIKGSWQPVGDLLIDSLQDHLEKVKALRGEITPLKENVSYVNDLARQLTTLGIQLSPYNLNTLEDLNTRWKLLQVAIEDRIRQLHEAHRDFGPASQHFLSTSVQGPWERAISPNKVPYYINHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQVINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPASSPQLSHDDTHSRIEHYASRLAEMENSNGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKQQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDTSTGLEEVMEQLNHSFPSSRGHNVGSLFHLADDLGRAMESLVSVMTDEEGAE

Target ORF information:

RefSeq Version	XM_005641030.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris dystrophin (DMD), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005641030.3

ORF Insert Sequence:

ATGCTTTGGT GGGAAGAAGT AGAGGACTGT TATGAAAGAG AAGATGTTCA AAAGAAAACA 
TTCACAAAAT GGATAAATGC ACAGTTTTCT AAGTTTGGGA AGCAGCACAT AGAGAACCTC 
TTCAGTGACC TACAGGATGG GAGACGCCTC CTAGACCTTT TGGAAGGCCT GACAGGGCAA 
AAACTGCCAA AAGAAAAAGG ATCCACAAGA GTTCATGCCC TGAACAATGT CAACAAGGCA 
CTGCGCGTCT TGCAGAAAAA TAATGTTGAT TTAGTGAACA TTGGAAGTAC TGACATAGTA 
GATGGAAATC ACAAACTGAC TCTTGGTTTG ATTTGGAATA TAATCCTCCA CTGGCAGGTC 
AAAAATGTAA TGAAAAATAT CATGGCTGGA TTGCAACAAA CCAACAGTGA AAAGATTCTC 
CTGAGCTGGG TCCGACAATC AACTCGTAAT TATCCACAGG TTAATGTCAT TAACTTCACC 
ACCAGCTGGT CTGATGGCCT GGCTTTGAAC GCTCTCATCC ACAGTCATAG GCCAGACCTG 
TTTGATTGGA ATAGTGTGGT TTGCCAGCAG TCAGCCACAC AACGCCTGGA ACATGCATTC 
AACATTGCCA AATATCAATT AGGCATAGAG AAACTGCTTG ATCCTGAAGA TGTTGCCACC 
ACTTATCCAG ATAAGAAGTC CATCTTAATG TATATCACAT CACTCTTCCA AGTTTTGCCT 
CAACAAGTGA GCATTGAAGC CATCCAGGAA GTGGAAATGT TGCCAAGGCC ATCAAAAGTT 
ACTAGAGAAG AACATTTTCA ATTACATCAT CAAATGCACT ATTCTCAACA GATCACAGTC 
AGTCTAGCAC AGGGCTATGA ACGAGCCCCT TCCTCTCCTA AGCCTCGGTT CAAGAGCTAT 
GCCTACACAC AGGCTGCTTA TGTCACCACT TCTGACCCCA CACGGAGCCC ACTTCCTTCA 
CAGCATTTGG AAACTCCTGA AGACAAGTCA TTTGGCCGGT CATTGACAGA GACCGAAGCA 
AACCTGGACA GTTATCAAAC AGCTTTGGAA GAAGTACTCT CGTGGCTTCT TTCAGCTGAG 
GATGCACTGC AAGCCCAAGG AGAGATTTCT AATGATGTCG AAGAAGTGAA AGAACAATTT 
CATACTCATG AGGGATATAT GATGGACTTG ACATCCCATC AGGGACGGGT CGGTAATGTT 
CTCCAACTGG GAAGTCAACT GATTGGAACA GGGAAATTAT CAGAAGATGA AGAAACCGAA 
GTGCAGGAAC AAATGAATCT CCTCAATTCA AGATGGGAAT GCCTCAGGGT AGCTAGCATG 
GAAAAACAAA GCAATTTACA TAAAGTTCTA ATGGATCTCC AGAATCAGCA ACTGAAAGAG 
TTAAATGACT GGCTAACCAA AACAGAAGAG AGAACAAGGA AAATGGAGAA GGAGCCCCTT 
GGACCTGATA TTGAAGACCT AAAACGCCAA GTACAACAAC ATAAGGTGCT TCAAGAAGAC 
TTAGAACAGG AACAAGTCAG GGTCAATTCC CTCACTCATA TGGTGGTGGT AGTCGATGAA 
TCTAGTGGAG ACCATGCAAC TGCTGCTTTG GAAGAACAAC TTAAGGTACT GGGAGATCGA 
TGGGCAAACA TCTGTAGGTG GACAGAAGAT CGCTGGGTTC TTTTACAAGA CATCCTCCTA 
AAATGGCAGC GTTTTACTGA AGAACAGTGC CTTTTCAGTG CATGGCTTTC GGAGAAGGAA 
GATGCAGTGA ACAAGATTCA CACAACTGGC TTTAAGGATC AAAGTGAAGT GTTATCAAAT 
CTTCAGAAAC TGGCTGTCTT AAAAACAGAT CTGGAAAAGA AGAAGCAAAC CATGGACAAA 
CTCTGCTCAC TCAACCAAGA CCTTCTTTCA GCGCTGAAAA ACACAGTGGT AGCCCACAAG 
ATGGAAGCAT GGCTGGACAA CTTTGCCCAG CGCTGGGATA ATTTAGTCCA GAAACTTGAA 
AAAAGTTCAG CACAGATTTC ACAGGCTGTC ACCACCACTC AGCCATCACT AACACAGACA 
ACTGTAATGG AAACAGTAAC TATGGTGACC ACGAGGGAAC ACATCTTGGT AAAGCATGCC 
CAAGAGGAAC TGCCACCACC ACCCCCTCAG AAGAAGAGGC AGATTATCGT GGATTCTGAA 
ATTAGGAAAA GGTTGGATGT CGATATAACT GAACTTCACA GTTGGATTAC TCGTTCAGAA 
GCTGTGTTGC AGAGTCCTGA ATTTGCAATC TATCGGAAGG AAGGCAACTT CTCAGACCTT 
AAAGAAAAAG TCAATGCCAT AGAGCGAGAA AAAGCCGAGA AGTTCAGAAA ACTGCAAGAT 
GCCAGCAGAT CAGCTCAGGC CCTGGTGGAA CAGATGGTGA ATGAGGGTGT TAATGCTGAC 
AGCATCAAAC AAGCCTCCGA ACAACTGAAC AGCCGGTGGA TAGAGTTCTG CCAATTGCTA 
AGCGAGAGAC TTAACTGGCT GGAGTATCAG AACAACATCA TCACTTTCTA TAATCAGCTA 
CAACAATTGG AGCAGATGAC AACTACTGCT GAAAACTGGT TGAAAACCCA GCCTACCACC 
ACATCAGAGC CAACAGCAAT TAAAAGCCAG TTAAAAATTT GTAAGGATGA AATCAACCGA 
CTGTCAGCTC TTCAGCCTCA AATCGAGCGA TTAAAAATTC AAAGCATAGC CCTGAAAGAG 
AAAGGACAAG GGCCAATGTT CCTGGATGCA GACTTTGTGG CCTTTACAAA TCATTTTAAC 
CAAGTCTTTG CTGATGTGCA GGCAAGAGAA AAAGAGCTAC AAACAATTTT TGACAGTTTG 
CCACCCATGC GCTATCAGGA GACTATGAGT ACCATCCTGA CATGGATCCA GCAGTCAGAA 
ACCAAACTCT CTATACCTCA GGTTACTGTC ACTGAATATG ACATCATGGA ACAGAGACTC 
GGAGAGCTAC AGGCTTTACA AAGCTCTCTG CAAGAGCAAC AAAATGGCCT AAACTATCTC 
AGCACCACTG TGAAAGAGAT GTCAAAGAAA GCACCACTGT CTGATATTAG TCGGAAATAT 
CAATCAGAAT TTGAAGAGAT TGAGGGACGT TGGAAGAAGC TGTCTTCCCA GCTGGTTGAA 
CATTGTCAAA AGTTGGAGGA GCAAATGGCT AAACTTCGAA AAATTCAGAA TCACATAAAA 
ACTCTGAAGA AATGGATCAC TGAAGTCGAT GTTTTCCTGA AGGAGGAATG GCCTGCCCTT 
GGGGATTCAG AAATTCTGAA AAGACAGCTG AAACAGTGCA GGCTTTTAGT CAATGACATT 
CAGACCATCC AGCCTAGTCT CAACAGTGTC AATGAAGGGG CTCAGAAGAT GAAGAATGAA 
GCAGAACCAG AGTTTGCTGG CAGACTTGAG ACAGAGCTCC GAGAACTTAA CACCCAGTGG 
GATTACATGT GCCGCCAGGT CTATGCCAGG AAGGAAGCCT TAAAAGGAGG TTTGGATAAA 
ACTGTAAGTC TTCAGAAAGA TCTGTCAGAG ATGCATGAGT GGATGACACA AGCTGAAGAA 
GAATACCTAG AGAGAGATTT CGAATACAAG ACCCCTGATG AATTACAGAC AGCAGTTGAA 
GAGATGAAGA GAGCTAAAGA AGAGGCCCAG CAAAAAGAAG CAAAAGTGAA ACTCCTAACC 
GAGTCCGTCA ATAGTGTCAT AGCTCAGGCT CCACCTGCAG CACAAGAGGC CTTAAAAAAG 
GAACTTGACA CTCTCACCAC CAACTACCAG TGGCTCTGCA CCAGGCTCAA TGGCAAATGC 
AAGACCTTGG AAGAAGTTTG GGCGTGCTGG CATGAGTTAT TGTCCTACTT GGAGAAGGCA 
AACAAGTGGC TAAGTGAAGT AGAAGTCAAG CTTAAAACCA CTGAAAATAT TTCTGGGGGA 
GCTGAGGAAA TCGCCGAGGT GCTTGATTCG CTTGAAAATT TGATGCAACA TTCAGAGGAT 
AACCCGAATC AGATTCGCAT ATTGGCACAG ACCTTGACAG ATGGTGGAGT CATGGATGAA 
CTGATCAATG AGGAGCTTGA GACATTTAAT TCTCGTTGGA GAGAACTCCA TGAAGAGGCT 
GTGAGGAGGC AAAAGTTGCT TGAGCAGAGT ATCCAGTCGG CCCAGGAGAT AGAAAAATCC 
TTGCACTTAA TTCAGGAGTC CCTCTCTTCC ATTGACAAGC AGTTGGCAGC TTATATTGCT 
GACAAAGTGG ATGCAGCTCA GATGCCTCAG GAAGCCCAGA AAATCCAATC AGATTTGACA 
AGTCATGAGA TCAGTTTAGA AGAAATGAAG AAACATAACC AGGGAAAGGA GACTGCCCAA 
AGGGTACTAT CCCAAATTGA TGTGGCACAG AAAAAATTGC AGGATGTTTC CATGAAGTTT 
CGATTATTCC AGAAGCCAGC CAATTTTGAG CAGCGCCTAC AAGAAAGTAA AATGATTTTA 
GATGAAGTGA AGATGCATTT ACCTGCGTTG GAAACAAAGA GTGTGGAACA GGAAGTAGTA 
CAGTCACAGT TAAATCATTG TGTGAACTTG TATAAAAGTC TGAGTGAAGT GAAGTCTGAA 
GTGGAAATGG TAATAAAAAC TGGACGTCAG ATTGTACAGA AGAAGCAGAC GGAAAACCCG 
AAAGAGCTTG ATGAAAGAGT TACAGCTTTG AAATTGCATT ATAATGAGCT GGGAGCAAAG 
GTGACAGAAA GAAAGCAACA GTTGGAAAAA TGCTTGAAAT TGTCCCGTAA GATGCGAAAG 
GAAATGAATG CCCTGACAGA ATGGCTGGCA GCGACAGATA TGGAACTGAC GAAGAGATCG 
GCAGTTGAAG GAATGCCTAG TAATTTGGAT TCTGAAGTTG CCTGGGGAAA GGCTACTCAG 
AAAGAGATTG AGAAACAGAA GGTTCACCTA AAGAGTGTCA CAGAGGTAGG AGAGGCCTTG 
AAAACGGTTT TGGGCAAGAA GGAAATGTTG GTGGAAGATA AACTGAGTCT TCTGAATAGT 
AACTGGATAG CCGTCACTTC CCGAGCAGAA GAGTGGTTAA ACCTTTTATT GGAATACCAG 
AAACACATGG AAAACTTTGA CCAGAATGTG GATTACATCA CAAACTGGAT CATTCAGGCT 
GATGCACTTT TGGATGAATC TGAGAAAAAG AAACCTCAGC AAAAAGAAGA CATACTTAAG 
CGTTTAAAGG CTGAAATGAA TGACATACGT CCAAAGGTGG ATTCTACACG TGACCAAGCA 
GCAAACCTGA TGGCAAACCG CGGCGACCAC TGCAGGAAAG TAGTAGAGCC CAAAATCTCA 
GAGCTCAACC ATCGATTTGC AGCCATTTCT CACAGAATTA AGACTGGAAA GGCCTCCATT 
CCTTTGAAGG AATTGGAGCA GTTTAACTCA GATATACAAA AATTGCTTGA ACCACTGGAG 
GCTGAAATTC AGCAGGGGGT GAATCTGAAA GAGGAAGACT TCAATAAAGA TATGAGTGAA 
GACAATGAGG GTACTGTAAA AGAATTGTTG CAAAGAGGAG ACAACTTACA ACAAAGAATC 
ACAGATGAGA GAAAGCGAGA GGAAATAAAG ATAAAACAAC AGCTGTTACA GACAAAACAT 
AATGCTCTCA AGGATTTGAG GTCTCAAAGA AGAAAAAAGG CTCTAGAAAT TTCTCACCAG 
TGGTATCAGT ACAAGAGGCA GGCTGATGAT CTCCTGAAAT GCTTGGATGA CATTGAAAAA 
AAATTAGCCA GCCTACCTGA ACCCAGAGAT GAAAGGAAAA TAAAGGAAAT TGATCGTGAA 
TTGCAGAAGA AGAAAGAGGA GCTGAATGCA GTGCGTAGGC AAGCTGAGGG CTTGTCTGAG 
GATGGGGCCG CAATGGCAGT GGAGCCAACT CAGATCCAGC TCAGCAAGCG CTGGCGGGAA 
ATTGAGAGCA AATTTGCTCA GTTTCGAAGA CTCAACTTTG CACAAATTCA CACTGTCCAT 
GAAGAGTCAG TGGTGGCGAT GACTGAAGAC ATGCCTTTGG AAATTTCTTA TGTGCCTTCT 
ACTTACCTGA CTGAGATCAC TCATGTCTCA CAAGCCCTAT CAGAAGTGGA AGAGCTTCTT 
AATGCTCCCG ACCTTTGTGC TCAAGATTTT GAAGATCTCT TTAAACAAGA GGAATCCTTG 
AAGAACATAA AAGACAGCCT GCAACAAATC TCAGGTCGGA TTGACATCAT TCACAATAAA 
AAGACAGCAG CATTGCACAG TGCCACTCCT GCAGAAAGGG CAAAGCTCCA GGAAGCTCTC 
TCACGGCTTG ATTTCCAATG GGAAAGAGTT AACAATATGT ACAAGGACCG ACAAGGGAGA 
TTTGACAGAT CTGTGGAAAA ATGGCGGCGG TTTCATTATG ATATGAAGAT ACTTAATCAA 
TGGCTAACAG AAGCTGAACA GTTTCTCAAA AAGACACAAA TTCCTGAGAA TTGGGAACAT 
GCCAAATACA AATGGTATCT TAAGGAACTC CAGGATGGCA TTGGACAGCG GCAAAGTGTT 
GTCAGGGTAT TGAATGCAAC TGGGGAAGAA ATAATTCAAC AGTCCTCAAA AACAGATGCC 
AGTATTCTCC AAGAAAAACT GGGAAGCCTG AATCTGCGGT GGCAGGAGGT CTGCAAACAG 
CTGGCAGAAA GAAAAAAGAG GCTAGAGGAA CAGAAGAATA TCTTGTCAGA ATTTCAAAGA 
GATGTAAATG AATTTGTTTT ATGGTTGGAA GAAGCGGATA ACGTTGCTAA TATTCCACTT 
GAACCTGGAA ATGAGCAGCA GCTAAAAGAA AAACTTGAAC AAGTCAAGTT ACTGGCAGAA 
GAGTTGCCCC TGCGCCAGGG AATTCTAAAA CAATTAAATG AAACTGGAGG AACAGTGCTT 
GTAAGTGCTC CCCTAAGCCC AGAAGAGCAA GATAAACTTG AAAATAAGCT CAAGCAGACA 
AATCTTCAGT GGATAAAGGT TTCTAGAAAT CTGCCTGAGA AGCAAGAAGA AATTGAGGCA 
CACGTAAAAG ACCTTGGACA GCTGGAAGAG CAGTTAAATC ATCTGCTTCT ATGGCTGTCT 
CCTATTAGGA ATCAGTTGGA AATTTACAAT CAGCCAAATC AAACAGGACC ATTTGACATC 
AAGGAAATTG AAGTAGCAGT TCAAGCTAAA CAGCCGGATG TGGAAGGGAT TTTGTCTAAA 
GGGCAGCATT TGTACAAGGA AAAACCAGCC ACTCAGCCAG CGAAGAGAAA GCTGGAAGAT 
CTCAGCTCTG ATTGGAAGGT GGTAACTCAG TTGCTTCAAG AGCTGCGGGC AAAGCAGCCT 
GGCCCAGCTC CTGGACTGAC CACTGTCAGA GCCCCTCCCA GTCAGACTGT TACTCTGGTG 
ACACAACCCG CGGTTACCAA GGAAACTGCC ATCTCCAAAC TAGAAATGCC ATCTTCATTG 
CTGTTGGAGG TACCTGCACT GGCAGATTTC AACCGAGCTT GGACAGAACT TACCGACTGG 
CTGTCTCTGC TTGATCGAGT TATAAAATCA CAGAGGGTGA TGGTGGGTGA TCTTGAAGAC 
ATTAACGAGA TGATCATCAA GCAGAAGGCA ACGCTGCAGG ATTTGGAACA GAGGCGCCCC 
CAGTTGGAAG AACTCATTAC CGCTGCCCAG AATTTGAAAA ACAAGACCAG CAATCAAGAG 
GCTAGAACAA TCATTACTGA TCGAATTGAA AGAATTCAGA GTCAGTGGGA TGAAGTACAG 
GAACATCTTC AGAACCGGAG GCAACAGTTG AATGAAATGT TAAAGGATTC CACACAATGG 
CTGGAAGCTA AAGAGGAGGC TGAGCAGGTG TTGGGGCAGG CCAGAGCCAA GCTTGAGTCA 
TGGAAGGAGG CTCCCTACAC AGTAGATGCA ATCCAAAAGA AAATCACAGA AACCAAGCAG 
TTGGCCAAAG ACCTCCGCCA GTGGCAGATA AATGTAGATG TTGCAAATGA TTTGGCACTG 
AAACTTCTCC GAGATTATTC TGCAGATGAT ACCAGAAAAG TACACATGAT AACAGAGAAC 
ATCAATGCCT CTTGGGCAAG CATCCATAAA AGATTGAGTG AGCGAGAGGC TGCTCTGGAA 
GAAACCCACA GATTACTGCA ACAGTTCCCC TTGGACCTGG AGAAGTTCCT TGCCTGGCTT 
ACAGAAGCCG AAACAACTGC CAACGTCCTG CAGGATGCCA CCCATAAGGA AAGGCTTCTA 
GAAGATTCCA AGGGAGTAAG AGAGCTGATG AAACAATGGC AAGATCTCCA AGGAGAAATC 
GAAGCTCACA CAGATATCTA TCACAACCTG GACGAAAATG GCCAAAAAGT CCTGAGATCC 
CTGGAAGGTT CTGACGATGC AGCCTTGTTG CAAAGACGTT TGGATAACAT GAACTTCAAG 
TGGAGCGAAC TTCGGAAAAA GTCTCTCAAC ATTAGGTCTC ACTTGGAAGC CAGTTCTGAC 
CAGTGGAAGC GTCTGCACCT TTCTCTTCAG GAACTTCTGG TATGGCTCCA GCTGAAAGAT 
GATGAGTTAA GCCGGCAGGC ACCCATTGGA GGAGACTTTC CAGCGGTGCA GAAGCAGAAT 
GATGTACACA GGGCCTTCAA GAGGGAATTG AAAACGAAAG AACCTGTAAT CATGAGTACT 
CTTGAGACTG TACGAATATT TCTGACAGAG CAGCCTTTAG AAGGACTAGA GAAACTCTAC 
CAGGAGCCCA GAGAGCTGCC TCCTGAAGAG AGAGCCCAGA ATGTCACACG GCTCCTACGA 
AAGCAAGCTG AGGAGGTCAA CACTCAGTGG GAAAAACTGA ACGTGCACTC TGCAGACTGG 
CAGAGAAAAA TAGACGAGGC CCTCGAAAGA CTCCAGGAGC TTCAGGAAGC AACAGATGAG 
CTGGATCTCA AACTACGTCA GGCAGAGGTG ATCAAAGGAT CCTGGCAGCC TGTGGGTGAT 
CTCCTCATTG ACTCTCTCCA AGATCACCTC GAAAAAGTCA AGGCGCTTCG AGGAGAAATT 
ACACCTCTGA AAGAGAATGT CAGCTACGTC AATGACCTTG CTCGCCAACT CACTACGTTG 
GGCATTCAGC TGTCACCATA TAACCTCAAC ACTCTGGAAG ACCTGAACAC CAGATGGAAG 
CTTCTGCAGG TGGCCATTGA GGACCGCATC AGGCAGCTGC ATGAAGCGCA CAGGGACTTT 
GGACCAGCCT CCCAGCACTT CCTTTCCACT TCTGTCCAGG GTCCCTGGGA GAGAGCCATC 
TCACCAAACA AAGTGCCCTA CTATATCAAC CACGAGACCC AAACAACTTG CTGGGACCAT 
CCCAAAATGA CAGAGCTCTA CCAGTCTTTA GCTGACCTGA ATAATGTCAG GTTCTCAGCT 
TACAGGACTG CCATGAAACT CCGAAGACTG CAGAAGGCCC TTTGCTTGGA TCTCTTGAGC 
CTATCGGCTG CATGCGATGC CTTGGACCAG CACAACCTCA AGCAAAATGA CCAGCCCATG 
GATATCCTGC AGGTCATTAA CTGTCTGACC ACTATTTATG ATCGCCTAGA GCAAGAGCAC 
AACAATCTGG TCAACGTCCC TCTCTGCGTG GATATGTGTC TCAATTGGCT GCTGAATGTT 
TATGACACGG GACGAACGGG GAGGATCCGG GTCCTGTCTT TTAAAACTGG CATCATTTCT 
CTGTGTAAAG CCCATTTGGA AGACAAGTAC AGATACCTCT TCAAGCAAGT GGCAAGTTCG 
ACAGGATTTT GTGACCAGCG CAGGCTGGGC CTCCTCCTGC ATGACTCTAT CCAGATCCCA 
AGACAGTTGG GTGAAGTCGC ATCGTTCGGG GGCAGTAACA TTGAGCCGAG TGTCAGGAGC 
TGCTTCCAGT TTGCTAATAA TAAGCCTGAG ATCGAAGCGG CCCTCTTCCT AGACTGGATG 
CGCCTGGAGC CCCAGTCCAT GGTGTGGCTG CCTGTCCTGC ACCGAGTGGC TGCCGCGGAA 
ACTGCCAAGC ACCAGGCCAA GTGCAACATC TGCAAGGAGT GTCCCATCAT CGGATTCAGG 
TACAGGAGTC TAAAGCACTT TAATTATGAC ATCTGCCAAA GTTGCTTTTT TTCTGGTCGA 
GTTGCAAAAG GCCATAAAAT GCACTATCCC ATGGTGGAAT ACTGCACTCC GACTACATCG 
GGAGAAGATG TCCGTGACTT TGCCAAGGTA CTAAAAAACA AATTTCGAAC CAAAAGGTAT 
TTTGCGAAGC ATCCCCGAAT GGGCTACCTG CCAGTGCAGA CTGTCTTAGA GGGGGACAAC 
ATGGAAACGC CTGCCTCGTC CCCTCAGCTT TCACACGATG ATACTCATTC ACGCATTGAG 
CATTATGCTA GCAGGCTAGC AGAAATGGAA AACAGCAATG GATCTTATCT AAATGATAGC 
ATCTCTCCTA ATGAGAGCAT AGATGATGAA CATTTGTTAA TCCAGCATTA CTGCCAAAGT 
TTGAACCAGG ACTCCCCCCT GAGCCAGCCT CGTAGTCCTG CCCAGATCTT GATTTCCTTA 
GAGAGTGAGG AAAGAGGGGA GCTAGAGAGA ATCCTAGCAG ATCTTGAGGA AGAAAACAGA 
AATCTGCAAG CAGAGTATGA TCGTCTAAAG CAGCAGCATG AACACAAAGG CCTGTCCCCA 
CTGCCATCCC CTCCTGAAAT GATGCCTACT TCTCCCCAAA GTCCCCGGGA TGCTGAGCTC 
ATCGCTGAGG CCAAGCTGCT GCGTCAACAC AAAGGCCGCC TGGAAGCCAG GATGCAAATC 
CTTGAAGACC ATAACAAACA ACTGGAATCC CAGTTACACA GGCTCAGGCA GCTGCTGGAG 
CAACCCCAGG CAGAGGCCAA GGTGAATGGT ACAACGGTGT CTTCTCCTTC TACCTCTCTT 
CAGAGGTCAG ATAGCAGTCA GCCTATGCTG CTCCGGGTGG TCGGCAGTCA GACTTCAGAA 
TCCATGGGCG AGGAAGACCT GCTCAGCCCT CCCCAGGACA CAAGCACAGG GTTAGAGGAA 
GTGATGGAGC AGCTCAACCA CTCCTTCCCT AGTTCCAGAG GACACAATGT AGGAAGCCTT 
TTCCACTTGG CAGATGATTT GGGCAGAGCG ATGGAGTCCT TAGTTTCAGT CATGACAGAT 
GAAGAAGGGG CAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMD ( XM_005641030.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005641087.1 Canis lupus familiaris dystrophin (DMD), transcript variant X5, mRNA.

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CloneID	OCe31974
Clone ID Related Accession (Same CDS sequence)	XM_005641030.3 , XM_005641030.2
Accession Version	XM_005641030.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	dystrophin isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005641030.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTGGT GGGAAGAAGT AGAGGACTGT TATGAAAGAG AAGATGTTCA AAAGAAAACA 
TTCACAAAAT GGATAAATGC ACAGTTTTCT AAGTTTGGGA AGCAGCACAT AGAGAACCTC 
TTCAGTGACC TACAGGATGG GAGACGCCTC CTAGACCTTT TGGAAGGCCT GACAGGGCAA 
AAACTGCCAA AAGAAAAAGG ATCCACAAGA GTTCATGCCC TGAACAATGT CAACAAGGCA 
CTGCGCGTCT TGCAGAAAAA TAATGTTGAT TTAGTGAACA TTGGAAGTAC TGACATAGTA 
GATGGAAATC ACAAACTGAC TCTTGGTTTG ATTTGGAATA TAATCCTCCA CTGGCAGGTC 
AAAAATGTAA TGAAAAATAT CATGGCTGGA TTGCAACAAA CCAACAGTGA AAAGATTCTC 
CTGAGCTGGG TCCGACAATC AACTCGTAAT TATCCACAGG TTAATGTCAT TAACTTCACC 
ACCAGCTGGT CTGATGGCCT GGCTTTGAAC GCTCTCATCC ACAGTCATAG GCCAGACCTG 
TTTGATTGGA ATAGTGTGGT TTGCCAGCAG TCAGCCACAC AACGCCTGGA ACATGCATTC 
AACATTGCCA AATATCAATT AGGCATAGAG AAACTGCTTG ATCCTGAAGA TGTTGCCACC 
ACTTATCCAG ATAAGAAGTC CATCTTAATG TATATCACAT CACTCTTCCA AGTTTTGCCT 
CAACAAGTGA GCATTGAAGC CATCCAGGAA GTGGAAATGT TGCCAAGGCC ATCAAAAGTT 
ACTAGAGAAG AACATTTTCA ATTACATCAT CAAATGCACT ATTCTCAACA GATCACAGTC 
AGTCTAGCAC AGGGCTATGA ACGAGCCCCT TCCTCTCCTA AGCCTCGGTT CAAGAGCTAT 
GCCTACACAC AGGCTGCTTA TGTCACCACT TCTGACCCCA CACGGAGCCC ACTTCCTTCA 
CAGCATTTGG AAACTCCTGA AGACAAGTCA TTTGGCCGGT CATTGACAGA GACCGAAGCA 
AACCTGGACA GTTATCAAAC AGCTTTGGAA GAAGTACTCT CGTGGCTTCT TTCAGCTGAG 
GATGCACTGC AAGCCCAAGG AGAGATTTCT AATGATGTCG AAGAAGTGAA AGAACAATTT 
CATACTCATG AGGGATATAT GATGGACTTG ACATCCCATC AGGGACGGGT CGGTAATGTT 
CTCCAACTGG GAAGTCAACT GATTGGAACA GGGAAATTAT CAGAAGATGA AGAAACCGAA 
GTGCAGGAAC AAATGAATCT CCTCAATTCA AGATGGGAAT GCCTCAGGGT AGCTAGCATG 
GAAAAACAAA GCAATTTACA TAAAGTTCTA ATGGATCTCC AGAATCAGCA ACTGAAAGAG 
TTAAATGACT GGCTAACCAA AACAGAAGAG AGAACAAGGA AAATGGAGAA GGAGCCCCTT 
GGACCTGATA TTGAAGACCT AAAACGCCAA GTACAACAAC ATAAGGTGCT TCAAGAAGAC 
TTAGAACAGG AACAAGTCAG GGTCAATTCC CTCACTCATA TGGTGGTGGT AGTCGATGAA 
TCTAGTGGAG ACCATGCAAC TGCTGCTTTG GAAGAACAAC TTAAGGTACT GGGAGATCGA 
TGGGCAAACA TCTGTAGGTG GACAGAAGAT CGCTGGGTTC TTTTACAAGA CATCCTCCTA 
AAATGGCAGC GTTTTACTGA AGAACAGTGC CTTTTCAGTG CATGGCTTTC GGAGAAGGAA 
GATGCAGTGA ACAAGATTCA CACAACTGGC TTTAAGGATC AAAGTGAAGT GTTATCAAAT 
CTTCAGAAAC TGGCTGTCTT AAAAACAGAT CTGGAAAAGA AGAAGCAAAC CATGGACAAA 
CTCTGCTCAC TCAACCAAGA CCTTCTTTCA GCGCTGAAAA ACACAGTGGT AGCCCACAAG 
ATGGAAGCAT GGCTGGACAA CTTTGCCCAG CGCTGGGATA ATTTAGTCCA GAAACTTGAA 
AAAAGTTCAG CACAGATTTC ACAGGCTGTC ACCACCACTC AGCCATCACT AACACAGACA 
ACTGTAATGG AAACAGTAAC TATGGTGACC ACGAGGGAAC ACATCTTGGT AAAGCATGCC 
CAAGAGGAAC TGCCACCACC ACCCCCTCAG AAGAAGAGGC AGATTATCGT GGATTCTGAA 
ATTAGGAAAA GGTTGGATGT CGATATAACT GAACTTCACA GTTGGATTAC TCGTTCAGAA 
GCTGTGTTGC AGAGTCCTGA ATTTGCAATC TATCGGAAGG AAGGCAACTT CTCAGACCTT 
AAAGAAAAAG TCAATGCCAT AGAGCGAGAA AAAGCCGAGA AGTTCAGAAA ACTGCAAGAT 
GCCAGCAGAT CAGCTCAGGC CCTGGTGGAA CAGATGGTGA ATGAGGGTGT TAATGCTGAC 
AGCATCAAAC AAGCCTCCGA ACAACTGAAC AGCCGGTGGA TAGAGTTCTG CCAATTGCTA 
AGCGAGAGAC TTAACTGGCT GGAGTATCAG AACAACATCA TCACTTTCTA TAATCAGCTA 
CAACAATTGG AGCAGATGAC AACTACTGCT GAAAACTGGT TGAAAACCCA GCCTACCACC 
ACATCAGAGC CAACAGCAAT TAAAAGCCAG TTAAAAATTT GTAAGGATGA AATCAACCGA 
CTGTCAGCTC TTCAGCCTCA AATCGAGCGA TTAAAAATTC AAAGCATAGC CCTGAAAGAG 
AAAGGACAAG GGCCAATGTT CCTGGATGCA GACTTTGTGG CCTTTACAAA TCATTTTAAC 
CAAGTCTTTG CTGATGTGCA GGCAAGAGAA AAAGAGCTAC AAACAATTTT TGACAGTTTG 
CCACCCATGC GCTATCAGGA GACTATGAGT ACCATCCTGA CATGGATCCA GCAGTCAGAA 
ACCAAACTCT CTATACCTCA GGTTACTGTC ACTGAATATG ACATCATGGA ACAGAGACTC 
GGAGAGCTAC AGGCTTTACA AAGCTCTCTG CAAGAGCAAC AAAATGGCCT AAACTATCTC 
AGCACCACTG TGAAAGAGAT GTCAAAGAAA GCACCACTGT CTGATATTAG TCGGAAATAT 
CAATCAGAAT TTGAAGAGAT TGAGGGACGT TGGAAGAAGC TGTCTTCCCA GCTGGTTGAA 
CATTGTCAAA AGTTGGAGGA GCAAATGGCT AAACTTCGAA AAATTCAGAA TCACATAAAA 
ACTCTGAAGA AATGGATCAC TGAAGTCGAT GTTTTCCTGA AGGAGGAATG GCCTGCCCTT 
GGGGATTCAG AAATTCTGAA AAGACAGCTG AAACAGTGCA GGCTTTTAGT CAATGACATT 
CAGACCATCC AGCCTAGTCT CAACAGTGTC AATGAAGGGG CTCAGAAGAT GAAGAATGAA 
GCAGAACCAG AGTTTGCTGG CAGACTTGAG ACAGAGCTCC GAGAACTTAA CACCCAGTGG 
GATTACATGT GCCGCCAGGT CTATGCCAGG AAGGAAGCCT TAAAAGGAGG TTTGGATAAA 
ACTGTAAGTC TTCAGAAAGA TCTGTCAGAG ATGCATGAGT GGATGACACA AGCTGAAGAA 
GAATACCTAG AGAGAGATTT CGAATACAAG ACCCCTGATG AATTACAGAC AGCAGTTGAA 
GAGATGAAGA GAGCTAAAGA AGAGGCCCAG CAAAAAGAAG CAAAAGTGAA ACTCCTAACC 
GAGTCCGTCA ATAGTGTCAT AGCTCAGGCT CCACCTGCAG CACAAGAGGC CTTAAAAAAG 
GAACTTGACA CTCTCACCAC CAACTACCAG TGGCTCTGCA CCAGGCTCAA TGGCAAATGC 
AAGACCTTGG AAGAAGTTTG GGCGTGCTGG CATGAGTTAT TGTCCTACTT GGAGAAGGCA 
AACAAGTGGC TAAGTGAAGT AGAAGTCAAG CTTAAAACCA CTGAAAATAT TTCTGGGGGA 
GCTGAGGAAA TCGCCGAGGT GCTTGATTCG CTTGAAAATT TGATGCAACA TTCAGAGGAT 
AACCCGAATC AGATTCGCAT ATTGGCACAG ACCTTGACAG ATGGTGGAGT CATGGATGAA 
CTGATCAATG AGGAGCTTGA GACATTTAAT TCTCGTTGGA GAGAACTCCA TGAAGAGGCT 
GTGAGGAGGC AAAAGTTGCT TGAGCAGAGT ATCCAGTCGG CCCAGGAGAT AGAAAAATCC 
TTGCACTTAA TTCAGGAGTC CCTCTCTTCC ATTGACAAGC AGTTGGCAGC TTATATTGCT 
GACAAAGTGG ATGCAGCTCA GATGCCTCAG GAAGCCCAGA AAATCCAATC AGATTTGACA 
AGTCATGAGA TCAGTTTAGA AGAAATGAAG AAACATAACC AGGGAAAGGA GACTGCCCAA 
AGGGTACTAT CCCAAATTGA TGTGGCACAG AAAAAATTGC AGGATGTTTC CATGAAGTTT 
CGATTATTCC AGAAGCCAGC CAATTTTGAG CAGCGCCTAC AAGAAAGTAA AATGATTTTA 
GATGAAGTGA AGATGCATTT ACCTGCGTTG GAAACAAAGA GTGTGGAACA GGAAGTAGTA 
CAGTCACAGT TAAATCATTG TGTGAACTTG TATAAAAGTC TGAGTGAAGT GAAGTCTGAA 
GTGGAAATGG TAATAAAAAC TGGACGTCAG ATTGTACAGA AGAAGCAGAC GGAAAACCCG 
AAAGAGCTTG ATGAAAGAGT TACAGCTTTG AAATTGCATT ATAATGAGCT GGGAGCAAAG 
GTGACAGAAA GAAAGCAACA GTTGGAAAAA TGCTTGAAAT TGTCCCGTAA GATGCGAAAG 
GAAATGAATG CCCTGACAGA ATGGCTGGCA GCGACAGATA TGGAACTGAC GAAGAGATCG 
GCAGTTGAAG GAATGCCTAG TAATTTGGAT TCTGAAGTTG CCTGGGGAAA GGCTACTCAG 
AAAGAGATTG AGAAACAGAA GGTTCACCTA AAGAGTGTCA CAGAGGTAGG AGAGGCCTTG 
AAAACGGTTT TGGGCAAGAA GGAAATGTTG GTGGAAGATA AACTGAGTCT TCTGAATAGT 
AACTGGATAG CCGTCACTTC CCGAGCAGAA GAGTGGTTAA ACCTTTTATT GGAATACCAG 
AAACACATGG AAAACTTTGA CCAGAATGTG GATTACATCA CAAACTGGAT CATTCAGGCT 
GATGCACTTT TGGATGAATC TGAGAAAAAG AAACCTCAGC AAAAAGAAGA CATACTTAAG 
CGTTTAAAGG CTGAAATGAA TGACATACGT CCAAAGGTGG ATTCTACACG TGACCAAGCA 
GCAAACCTGA TGGCAAACCG CGGCGACCAC TGCAGGAAAG TAGTAGAGCC CAAAATCTCA 
GAGCTCAACC ATCGATTTGC AGCCATTTCT CACAGAATTA AGACTGGAAA GGCCTCCATT 
CCTTTGAAGG AATTGGAGCA GTTTAACTCA GATATACAAA AATTGCTTGA ACCACTGGAG 
GCTGAAATTC AGCAGGGGGT GAATCTGAAA GAGGAAGACT TCAATAAAGA TATGAGTGAA 
GACAATGAGG GTACTGTAAA AGAATTGTTG CAAAGAGGAG ACAACTTACA ACAAAGAATC 
ACAGATGAGA GAAAGCGAGA GGAAATAAAG ATAAAACAAC AGCTGTTACA GACAAAACAT 
AATGCTCTCA AGGATTTGAG GTCTCAAAGA AGAAAAAAGG CTCTAGAAAT TTCTCACCAG 
TGGTATCAGT ACAAGAGGCA GGCTGATGAT CTCCTGAAAT GCTTGGATGA CATTGAAAAA 
AAATTAGCCA GCCTACCTGA ACCCAGAGAT GAAAGGAAAA TAAAGGAAAT TGATCGTGAA 
TTGCAGAAGA AGAAAGAGGA GCTGAATGCA GTGCGTAGGC AAGCTGAGGG CTTGTCTGAG 
GATGGGGCCG CAATGGCAGT GGAGCCAACT CAGATCCAGC TCAGCAAGCG CTGGCGGGAA 
ATTGAGAGCA AATTTGCTCA GTTTCGAAGA CTCAACTTTG CACAAATTCA CACTGTCCAT 
GAAGAGTCAG TGGTGGCGAT GACTGAAGAC ATGCCTTTGG AAATTTCTTA TGTGCCTTCT 
ACTTACCTGA CTGAGATCAC TCATGTCTCA CAAGCCCTAT CAGAAGTGGA AGAGCTTCTT 
AATGCTCCCG ACCTTTGTGC TCAAGATTTT GAAGATCTCT TTAAACAAGA GGAATCCTTG 
AAGAACATAA AAGACAGCCT GCAACAAATC TCAGGTCGGA TTGACATCAT TCACAATAAA 
AAGACAGCAG CATTGCACAG TGCCACTCCT GCAGAAAGGG CAAAGCTCCA GGAAGCTCTC 
TCACGGCTTG ATTTCCAATG GGAAAGAGTT AACAATATGT ACAAGGACCG ACAAGGGAGA 
TTTGACAGAT CTGTGGAAAA ATGGCGGCGG TTTCATTATG ATATGAAGAT ACTTAATCAA 
TGGCTAACAG AAGCTGAACA GTTTCTCAAA AAGACACAAA TTCCTGAGAA TTGGGAACAT 
GCCAAATACA AATGGTATCT TAAGGAACTC CAGGATGGCA TTGGACAGCG GCAAAGTGTT 
GTCAGGGTAT TGAATGCAAC TGGGGAAGAA ATAATTCAAC AGTCCTCAAA AACAGATGCC 
AGTATTCTCC AAGAAAAACT GGGAAGCCTG AATCTGCGGT GGCAGGAGGT CTGCAAACAG 
CTGGCAGAAA GAAAAAAGAG GCTAGAGGAA CAGAAGAATA TCTTGTCAGA ATTTCAAAGA 
GATGTAAATG AATTTGTTTT ATGGTTGGAA GAAGCGGATA ACGTTGCTAA TATTCCACTT 
GAACCTGGAA ATGAGCAGCA GCTAAAAGAA AAACTTGAAC AAGTCAAGTT ACTGGCAGAA 
GAGTTGCCCC TGCGCCAGGG AATTCTAAAA CAATTAAATG AAACTGGAGG AACAGTGCTT 
GTAAGTGCTC CCCTAAGCCC AGAAGAGCAA GATAAACTTG AAAATAAGCT CAAGCAGACA 
AATCTTCAGT GGATAAAGGT TTCTAGAAAT CTGCCTGAGA AGCAAGAAGA AATTGAGGCA 
CACGTAAAAG ACCTTGGACA GCTGGAAGAG CAGTTAAATC ATCTGCTTCT ATGGCTGTCT 
CCTATTAGGA ATCAGTTGGA AATTTACAAT CAGCCAAATC AAACAGGACC ATTTGACATC 
AAGGAAATTG AAGTAGCAGT TCAAGCTAAA CAGCCGGATG TGGAAGGGAT TTTGTCTAAA 
GGGCAGCATT TGTACAAGGA AAAACCAGCC ACTCAGCCAG CGAAGAGAAA GCTGGAAGAT 
CTCAGCTCTG ATTGGAAGGT GGTAACTCAG TTGCTTCAAG AGCTGCGGGC AAAGCAGCCT 
GGCCCAGCTC CTGGACTGAC CACTGTCAGA GCCCCTCCCA GTCAGACTGT TACTCTGGTG 
ACACAACCCG CGGTTACCAA GGAAACTGCC ATCTCCAAAC TAGAAATGCC ATCTTCATTG 
CTGTTGGAGG TACCTGCACT GGCAGATTTC AACCGAGCTT GGACAGAACT TACCGACTGG 
CTGTCTCTGC TTGATCGAGT TATAAAATCA CAGAGGGTGA TGGTGGGTGA TCTTGAAGAC 
ATTAACGAGA TGATCATCAA GCAGAAGGCA ACGCTGCAGG ATTTGGAACA GAGGCGCCCC 
CAGTTGGAAG AACTCATTAC CGCTGCCCAG AATTTGAAAA ACAAGACCAG CAATCAAGAG 
GCTAGAACAA TCATTACTGA TCGAATTGAA AGAATTCAGA GTCAGTGGGA TGAAGTACAG 
GAACATCTTC AGAACCGGAG GCAACAGTTG AATGAAATGT TAAAGGATTC CACACAATGG 
CTGGAAGCTA AAGAGGAGGC TGAGCAGGTG TTGGGGCAGG CCAGAGCCAA GCTTGAGTCA 
TGGAAGGAGG CTCCCTACAC AGTAGATGCA ATCCAAAAGA AAATCACAGA AACCAAGCAG 
TTGGCCAAAG ACCTCCGCCA GTGGCAGATA AATGTAGATG TTGCAAATGA TTTGGCACTG 
AAACTTCTCC GAGATTATTC TGCAGATGAT ACCAGAAAAG TACACATGAT AACAGAGAAC 
ATCAATGCCT CTTGGGCAAG CATCCATAAA AGATTGAGTG AGCGAGAGGC TGCTCTGGAA 
GAAACCCACA GATTACTGCA ACAGTTCCCC TTGGACCTGG AGAAGTTCCT TGCCTGGCTT 
ACAGAAGCCG AAACAACTGC CAACGTCCTG CAGGATGCCA CCCATAAGGA AAGGCTTCTA 
GAAGATTCCA AGGGAGTAAG AGAGCTGATG AAACAATGGC AAGATCTCCA AGGAGAAATC 
GAAGCTCACA CAGATATCTA TCACAACCTG GACGAAAATG GCCAAAAAGT CCTGAGATCC 
CTGGAAGGTT CTGACGATGC AGCCTTGTTG CAAAGACGTT TGGATAACAT GAACTTCAAG 
TGGAGCGAAC TTCGGAAAAA GTCTCTCAAC ATTAGGTCTC ACTTGGAAGC CAGTTCTGAC 
CAGTGGAAGC GTCTGCACCT TTCTCTTCAG GAACTTCTGG TATGGCTCCA GCTGAAAGAT 
GATGAGTTAA GCCGGCAGGC ACCCATTGGA GGAGACTTTC CAGCGGTGCA GAAGCAGAAT 
GATGTACACA GGGCCTTCAA GAGGGAATTG AAAACGAAAG AACCTGTAAT CATGAGTACT 
CTTGAGACTG TACGAATATT TCTGACAGAG CAGCCTTTAG AAGGACTAGA GAAACTCTAC 
CAGGAGCCCA GAGAGCTGCC TCCTGAAGAG AGAGCCCAGA ATGTCACACG GCTCCTACGA 
AAGCAAGCTG AGGAGGTCAA CACTCAGTGG GAAAAACTGA ACGTGCACTC TGCAGACTGG 
CAGAGAAAAA TAGACGAGGC CCTCGAAAGA CTCCAGGAGC TTCAGGAAGC AACAGATGAG 
CTGGATCTCA AACTACGTCA GGCAGAGGTG ATCAAAGGAT CCTGGCAGCC TGTGGGTGAT 
CTCCTCATTG ACTCTCTCCA AGATCACCTC GAAAAAGTCA AGGCGCTTCG AGGAGAAATT 
ACACCTCTGA AAGAGAATGT CAGCTACGTC AATGACCTTG CTCGCCAACT CACTACGTTG 
GGCATTCAGC TGTCACCATA TAACCTCAAC ACTCTGGAAG ACCTGAACAC CAGATGGAAG 
CTTCTGCAGG TGGCCATTGA GGACCGCATC AGGCAGCTGC ATGAAGCGCA CAGGGACTTT 
GGACCAGCCT CCCAGCACTT CCTTTCCACT TCTGTCCAGG GTCCCTGGGA GAGAGCCATC 
TCACCAAACA AAGTGCCCTA CTATATCAAC CACGAGACCC AAACAACTTG CTGGGACCAT 
CCCAAAATGA CAGAGCTCTA CCAGTCTTTA GCTGACCTGA ATAATGTCAG GTTCTCAGCT 
TACAGGACTG CCATGAAACT CCGAAGACTG CAGAAGGCCC TTTGCTTGGA TCTCTTGAGC 
CTATCGGCTG CATGCGATGC CTTGGACCAG CACAACCTCA AGCAAAATGA CCAGCCCATG 
GATATCCTGC AGGTCATTAA CTGTCTGACC ACTATTTATG ATCGCCTAGA GCAAGAGCAC 
AACAATCTGG TCAACGTCCC TCTCTGCGTG GATATGTGTC TCAATTGGCT GCTGAATGTT 
TATGACACGG GACGAACGGG GAGGATCCGG GTCCTGTCTT TTAAAACTGG CATCATTTCT 
CTGTGTAAAG CCCATTTGGA AGACAAGTAC AGATACCTCT TCAAGCAAGT GGCAAGTTCG 
ACAGGATTTT GTGACCAGCG CAGGCTGGGC CTCCTCCTGC ATGACTCTAT CCAGATCCCA 
AGACAGTTGG GTGAAGTCGC ATCGTTCGGG GGCAGTAACA TTGAGCCGAG TGTCAGGAGC 
TGCTTCCAGT TTGCTAATAA TAAGCCTGAG ATCGAAGCGG CCCTCTTCCT AGACTGGATG 
CGCCTGGAGC CCCAGTCCAT GGTGTGGCTG CCTGTCCTGC ACCGAGTGGC TGCCGCGGAA 
ACTGCCAAGC ACCAGGCCAA GTGCAACATC TGCAAGGAGT GTCCCATCAT CGGATTCAGG 
TACAGGAGTC TAAAGCACTT TAATTATGAC ATCTGCCAAA GTTGCTTTTT TTCTGGTCGA 
GTTGCAAAAG GCCATAAAAT GCACTATCCC ATGGTGGAAT ACTGCACTCC GACTACATCG 
GGAGAAGATG TCCGTGACTT TGCCAAGGTA CTAAAAAACA AATTTCGAAC CAAAAGGTAT 
TTTGCGAAGC ATCCCCGAAT GGGCTACCTG CCAGTGCAGA CTGTCTTAGA GGGGGACAAC 
ATGGAAACGC CTGCCTCGTC CCCTCAGCTT TCACACGATG ATACTCATTC ACGCATTGAG 
CATTATGCTA GCAGGCTAGC AGAAATGGAA AACAGCAATG GATCTTATCT AAATGATAGC 
ATCTCTCCTA ATGAGAGCAT AGATGATGAA CATTTGTTAA TCCAGCATTA CTGCCAAAGT 
TTGAACCAGG ACTCCCCCCT GAGCCAGCCT CGTAGTCCTG CCCAGATCTT GATTTCCTTA 
GAGAGTGAGG AAAGAGGGGA GCTAGAGAGA ATCCTAGCAG ATCTTGAGGA AGAAAACAGA 
AATCTGCAAG CAGAGTATGA TCGTCTAAAG CAGCAGCATG AACACAAAGG CCTGTCCCCA 
CTGCCATCCC CTCCTGAAAT GATGCCTACT TCTCCCCAAA GTCCCCGGGA TGCTGAGCTC 
ATCGCTGAGG CCAAGCTGCT GCGTCAACAC AAAGGCCGCC TGGAAGCCAG GATGCAAATC 
CTTGAAGACC ATAACAAACA ACTGGAATCC CAGTTACACA GGCTCAGGCA GCTGCTGGAG 
CAACCCCAGG CAGAGGCCAA GGTGAATGGT ACAACGGTGT CTTCTCCTTC TACCTCTCTT 
CAGAGGTCAG ATAGCAGTCA GCCTATGCTG CTCCGGGTGG TCGGCAGTCA GACTTCAGAA 
TCCATGGGCG AGGAAGACCT GCTCAGCCCT CCCCAGGACA CAAGCACAGG GTTAGAGGAA 
GTGATGGAGC AGCTCAACCA CTCCTTCCCT AGTTCCAGAG GACACAATGT AGGAAGCCTT 
TTCCACTTGG CAGATGATTT GGGCAGAGCG ATGGAGTCCT TAGTTTCAGT CATGACAGAT 
GAAGAAGGGG CAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005641087.1
CDS	248..11305
Translation	MLWWEEVEDCYEREDVQKKTFTKWINAQFSKFGKQHIENLFSDLQDGRRLLDLLEGLTGQKLPKEKGSTRVHALNNVNKALRVLQKNNVDLVNIGSTDIVDGNHKLTLGLIWNIILHWQVKNVMKNIMAGLQQTNSEKILLSWVRQSTRNYPQVNVINFTTSWSDGLALNALIHSHRPDLFDWNSVVCQQSATQRLEHAFNIAKYQLGIEKLLDPEDVATTYPDKKSILMYITSLFQVLPQQVSIEAIQEVEMLPRPSKVTREEHFQLHHQMHYSQQITVSLAQGYERAPSSPKPRFKSYAYTQAAYVTTSDPTRSPLPSQHLETPEDKSFGRSLTETEANLDSYQTALEEVLSWLLSAEDALQAQGEISNDVEEVKEQFHTHEGYMMDLTSHQGRVGNVLQLGSQLIGTGKLSEDEETEVQEQMNLLNSRWECLRVASMEKQSNLHKVLMDLQNQQLKELNDWLTKTEERTRKMEKEPLGPDIEDLKRQVQQHKVLQEDLEQEQVRVNSLTHMVVVVDESSGDHATAALEEQLKVLGDRWANICRWTEDRWVLLQDILLKWQRFTEEQCLFSAWLSEKEDAVNKIHTTGFKDQSEVLSNLQKLAVLKTDLEKKKQTMDKLCSLNQDLLSALKNTVVAHKMEAWLDNFAQRWDNLVQKLEKSSAQISQAVTTTQPSLTQTTVMETVTMVTTREHILVKHAQEELPPPPPQKKRQIIVDSEIRKRLDVDITELHSWITRSEAVLQSPEFAIYRKEGNFSDLKEKVNAIEREKAEKFRKLQDASRSAQALVEQMVNEGVNADSIKQASEQLNSRWIEFCQLLSERLNWLEYQNNIITFYNQLQQLEQMTTTAENWLKTQPTTTSEPTAIKSQLKICKDEINRLSALQPQIERLKIQSIALKEKGQGPMFLDADFVAFTNHFNQVFADVQAREKELQTIFDSLPPMRYQETMSTILTWIQQSETKLSIPQVTVTEYDIMEQRLGELQALQSSLQEQQNGLNYLSTTVKEMSKKAPLSDISRKYQSEFEEIEGRWKKLSSQLVEHCQKLEEQMAKLRKIQNHIKTLKKWITEVDVFLKEEWPALGDSEILKRQLKQCRLLVNDIQTIQPSLNSVNEGAQKMKNEAEPEFAGRLETELRELNTQWDYMCRQVYARKEALKGGLDKTVSLQKDLSEMHEWMTQAEEEYLERDFEYKTPDELQTAVEEMKRAKEEAQQKEAKVKLLTESVNSVIAQAPPAAQEALKKELDTLTTNYQWLCTRLNGKCKTLEEVWACWHELLSYLEKANKWLSEVEVKLKTTENISGGAEEIAEVLDSLENLMQHSEDNPNQIRILAQTLTDGGVMDELINEELETFNSRWRELHEEAVRRQKLLEQSIQSAQEIEKSLHLIQESLSSIDKQLAAYIADKVDAAQMPQEAQKIQSDLTSHEISLEEMKKHNQGKETAQRVLSQIDVAQKKLQDVSMKFRLFQKPANFEQRLQESKMILDEVKMHLPALETKSVEQEVVQSQLNHCVNLYKSLSEVKSEVEMVIKTGRQIVQKKQTENPKELDERVTALKLHYNELGAKVTERKQQLEKCLKLSRKMRKEMNALTEWLAATDMELTKRSAVEGMPSNLDSEVAWGKATQKEIEKQKVHLKSVTEVGEALKTVLGKKEMLVEDKLSLLNSNWIAVTSRAEEWLNLLLEYQKHMENFDQNVDYITNWIIQADALLDESEKKKPQQKEDILKRLKAEMNDIRPKVDSTRDQAANLMANRGDHCRKVVEPKISELNHRFAAISHRIKTGKASIPLKELEQFNSDIQKLLEPLEAEIQQGVNLKEEDFNKDMSEDNEGTVKELLQRGDNLQQRITDERKREEIKIKQQLLQTKHNALKDLRSQRRKKALEISHQWYQYKRQADDLLKCLDDIEKKLASLPEPRDERKIKEIDRELQKKKEELNAVRRQAEGLSEDGAAMAVEPTQIQLSKRWREIESKFAQFRRLNFAQIHTVHEESVVAMTEDMPLEISYVPSTYLTEITHVSQALSEVEELLNAPDLCAQDFEDLFKQEESLKNIKDSLQQISGRIDIIHNKKTAALHSATPAERAKLQEALSRLDFQWERVNNMYKDRQGRFDRSVEKWRRFHYDMKILNQWLTEAEQFLKKTQIPENWEHAKYKWYLKELQDGIGQRQSVVRVLNATGEEIIQQSSKTDASILQEKLGSLNLRWQEVCKQLAERKKRLEEQKNILSEFQRDVNEFVLWLEEADNVANIPLEPGNEQQLKEKLEQVKLLAEELPLRQGILKQLNETGGTVLVSAPLSPEEQDKLENKLKQTNLQWIKVSRNLPEKQEEIEAHVKDLGQLEEQLNHLLLWLSPIRNQLEIYNQPNQTGPFDIKEIEVAVQAKQPDVEGILSKGQHLYKEKPATQPAKRKLEDLSSDWKVVTQLLQELRAKQPGPAPGLTTVRAPPSQTVTLVTQPAVTKETAISKLEMPSSLLLEVPALADFNRAWTELTDWLSLLDRVIKSQRVMVGDLEDINEMIIKQKATLQDLEQRRPQLEELITAAQNLKNKTSNQEARTIITDRIERIQSQWDEVQEHLQNRRQQLNEMLKDSTQWLEAKEEAEQVLGQARAKLESWKEAPYTVDAIQKKITETKQLAKDLRQWQINVDVANDLALKLLRDYSADDTRKVHMITENINASWASIHKRLSEREAALEETHRLLQQFPLDLEKFLAWLTEAETTANVLQDATHKERLLEDSKGVRELMKQWQDLQGEIEAHTDIYHNLDENGQKVLRSLEGSDDAALLQRRLDNMNFKWSELRKKSLNIRSHLEASSDQWKRLHLSLQELLVWLQLKDDELSRQAPIGGDFPAVQKQNDVHRAFKRELKTKEPVIMSTLETVRIFLTEQPLEGLEKLYQEPRELPPEERAQNVTRLLRKQAEEVNTQWEKLNVHSADWQRKIDEALERLQELQEATDELDLKLRQAEVIKGSWQPVGDLLIDSLQDHLEKVKALRGEITPLKENVSYVNDLARQLTTLGIQLSPYNLNTLEDLNTRWKLLQVAIEDRIRQLHEAHRDFGPASQHFLSTSVQGPWERAISPNKVPYYINHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQVINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPASSPQLSHDDTHSRIEHYASRLAEMENSNGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKQQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDTSTGLEEVMEQLNHSFPSSRGHNVGSLFHLADDLGRAMESLVSVMTDEEGAE

Target ORF information:

RefSeq Version	XM_005641030.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris dystrophin (DMD), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005641030.2

ORF Insert Sequence:

ATGCTTTGGT GGGAAGAAGT AGAGGACTGT TATGAAAGAG AAGATGTTCA AAAGAAAACA 
TTCACAAAAT GGATAAATGC ACAGTTTTCT AAGTTTGGGA AGCAGCACAT AGAGAACCTC 
TTCAGTGACC TACAGGATGG GAGACGCCTC CTAGACCTTT TGGAAGGCCT GACAGGGCAA 
AAACTGCCAA AAGAAAAAGG ATCCACAAGA GTTCATGCCC TGAACAATGT CAACAAGGCA 
CTGCGCGTCT TGCAGAAAAA TAATGTTGAT TTAGTGAACA TTGGAAGTAC TGACATAGTA 
GATGGAAATC ACAAACTGAC TCTTGGTTTG ATTTGGAATA TAATCCTCCA CTGGCAGGTC 
AAAAATGTAA TGAAAAATAT CATGGCTGGA TTGCAACAAA CCAACAGTGA AAAGATTCTC 
CTGAGCTGGG TCCGACAATC AACTCGTAAT TATCCACAGG TTAATGTCAT TAACTTCACC 
ACCAGCTGGT CTGATGGCCT GGCTTTGAAC GCTCTCATCC ACAGTCATAG GCCAGACCTG 
TTTGATTGGA ATAGTGTGGT TTGCCAGCAG TCAGCCACAC AACGCCTGGA ACATGCATTC 
AACATTGCCA AATATCAATT AGGCATAGAG AAACTGCTTG ATCCTGAAGA TGTTGCCACC 
ACTTATCCAG ATAAGAAGTC CATCTTAATG TATATCACAT CACTCTTCCA AGTTTTGCCT 
CAACAAGTGA GCATTGAAGC CATCCAGGAA GTGGAAATGT TGCCAAGGCC ATCAAAAGTT 
ACTAGAGAAG AACATTTTCA ATTACATCAT CAAATGCACT ATTCTCAACA GATCACAGTC 
AGTCTAGCAC AGGGCTATGA ACGAGCCCCT TCCTCTCCTA AGCCTCGGTT CAAGAGCTAT 
GCCTACACAC AGGCTGCTTA TGTCACCACT TCTGACCCCA CACGGAGCCC ACTTCCTTCA 
CAGCATTTGG AAACTCCTGA AGACAAGTCA TTTGGCCGGT CATTGACAGA GACCGAAGCA 
AACCTGGACA GTTATCAAAC AGCTTTGGAA GAAGTACTCT CGTGGCTTCT TTCAGCTGAG 
GATGCACTGC AAGCCCAAGG AGAGATTTCT AATGATGTCG AAGAAGTGAA AGAACAATTT 
CATACTCATG AGGGATATAT GATGGACTTG ACATCCCATC AGGGACGGGT CGGTAATGTT 
CTCCAACTGG GAAGTCAACT GATTGGAACA GGGAAATTAT CAGAAGATGA AGAAACCGAA 
GTGCAGGAAC AAATGAATCT CCTCAATTCA AGATGGGAAT GCCTCAGGGT AGCTAGCATG 
GAAAAACAAA GCAATTTACA TAAAGTTCTA ATGGATCTCC AGAATCAGCA ACTGAAAGAG 
TTAAATGACT GGCTAACCAA AACAGAAGAG AGAACAAGGA AAATGGAGAA GGAGCCCCTT 
GGACCTGATA TTGAAGACCT AAAACGCCAA GTACAACAAC ATAAGGTGCT TCAAGAAGAC 
TTAGAACAGG AACAAGTCAG GGTCAATTCC CTCACTCATA TGGTGGTGGT AGTCGATGAA 
TCTAGTGGAG ACCATGCAAC TGCTGCTTTG GAAGAACAAC TTAAGGTACT GGGAGATCGA 
TGGGCAAACA TCTGTAGGTG GACAGAAGAT CGCTGGGTTC TTTTACAAGA CATCCTCCTA 
AAATGGCAGC GTTTTACTGA AGAACAGTGC CTTTTCAGTG CATGGCTTTC GGAGAAGGAA 
GATGCAGTGA ACAAGATTCA CACAACTGGC TTTAAGGATC AAAGTGAAGT GTTATCAAAT 
CTTCAGAAAC TGGCTGTCTT AAAAACAGAT CTGGAAAAGA AGAAGCAAAC CATGGACAAA 
CTCTGCTCAC TCAACCAAGA CCTTCTTTCA GCGCTGAAAA ACACAGTGGT AGCCCACAAG 
ATGGAAGCAT GGCTGGACAA CTTTGCCCAG CGCTGGGATA ATTTAGTCCA GAAACTTGAA 
AAAAGTTCAG CACAGATTTC ACAGGCTGTC ACCACCACTC AGCCATCACT AACACAGACA 
ACTGTAATGG AAACAGTAAC TATGGTGACC ACGAGGGAAC ACATCTTGGT AAAGCATGCC 
CAAGAGGAAC TGCCACCACC ACCCCCTCAG AAGAAGAGGC AGATTATCGT GGATTCTGAA 
ATTAGGAAAA GGTTGGATGT CGATATAACT GAACTTCACA GTTGGATTAC TCGTTCAGAA 
GCTGTGTTGC AGAGTCCTGA ATTTGCAATC TATCGGAAGG AAGGCAACTT CTCAGACCTT 
AAAGAAAAAG TCAATGCCAT AGAGCGAGAA AAAGCCGAGA AGTTCAGAAA ACTGCAAGAT 
GCCAGCAGAT CAGCTCAGGC CCTGGTGGAA CAGATGGTGA ATGAGGGTGT TAATGCTGAC 
AGCATCAAAC AAGCCTCCGA ACAACTGAAC AGCCGGTGGA TAGAGTTCTG CCAATTGCTA 
AGCGAGAGAC TTAACTGGCT GGAGTATCAG AACAACATCA TCACTTTCTA TAATCAGCTA 
CAACAATTGG AGCAGATGAC AACTACTGCT GAAAACTGGT TGAAAACCCA GCCTACCACC 
ACATCAGAGC CAACAGCAAT TAAAAGCCAG TTAAAAATTT GTAAGGATGA AATCAACCGA 
CTGTCAGCTC TTCAGCCTCA AATCGAGCGA TTAAAAATTC AAAGCATAGC CCTGAAAGAG 
AAAGGACAAG GGCCAATGTT CCTGGATGCA GACTTTGTGG CCTTTACAAA TCATTTTAAC 
CAAGTCTTTG CTGATGTGCA GGCAAGAGAA AAAGAGCTAC AAACAATTTT TGACAGTTTG 
CCACCCATGC GCTATCAGGA GACTATGAGT ACCATCCTGA CATGGATCCA GCAGTCAGAA 
ACCAAACTCT CTATACCTCA GGTTACTGTC ACTGAATATG ACATCATGGA ACAGAGACTC 
GGAGAGCTAC AGGCTTTACA AAGCTCTCTG CAAGAGCAAC AAAATGGCCT AAACTATCTC 
AGCACCACTG TGAAAGAGAT GTCAAAGAAA GCACCACTGT CTGATATTAG TCGGAAATAT 
CAATCAGAAT TTGAAGAGAT TGAGGGACGT TGGAAGAAGC TGTCTTCCCA GCTGGTTGAA 
CATTGTCAAA AGTTGGAGGA GCAAATGGCT AAACTTCGAA AAATTCAGAA TCACATAAAA 
ACTCTGAAGA AATGGATCAC TGAAGTCGAT GTTTTCCTGA AGGAGGAATG GCCTGCCCTT 
GGGGATTCAG AAATTCTGAA AAGACAGCTG AAACAGTGCA GGCTTTTAGT CAATGACATT 
CAGACCATCC AGCCTAGTCT CAACAGTGTC AATGAAGGGG CTCAGAAGAT GAAGAATGAA 
GCAGAACCAG AGTTTGCTGG CAGACTTGAG ACAGAGCTCC GAGAACTTAA CACCCAGTGG 
GATTACATGT GCCGCCAGGT CTATGCCAGG AAGGAAGCCT TAAAAGGAGG TTTGGATAAA 
ACTGTAAGTC TTCAGAAAGA TCTGTCAGAG ATGCATGAGT GGATGACACA AGCTGAAGAA 
GAATACCTAG AGAGAGATTT CGAATACAAG ACCCCTGATG AATTACAGAC AGCAGTTGAA 
GAGATGAAGA GAGCTAAAGA AGAGGCCCAG CAAAAAGAAG CAAAAGTGAA ACTCCTAACC 
GAGTCCGTCA ATAGTGTCAT AGCTCAGGCT CCACCTGCAG CACAAGAGGC CTTAAAAAAG 
GAACTTGACA CTCTCACCAC CAACTACCAG TGGCTCTGCA CCAGGCTCAA TGGCAAATGC 
AAGACCTTGG AAGAAGTTTG GGCGTGCTGG CATGAGTTAT TGTCCTACTT GGAGAAGGCA 
AACAAGTGGC TAAGTGAAGT AGAAGTCAAG CTTAAAACCA CTGAAAATAT TTCTGGGGGA 
GCTGAGGAAA TCGCCGAGGT GCTTGATTCG CTTGAAAATT TGATGCAACA TTCAGAGGAT 
AACCCGAATC AGATTCGCAT ATTGGCACAG ACCTTGACAG ATGGTGGAGT CATGGATGAA 
CTGATCAATG AGGAGCTTGA GACATTTAAT TCTCGTTGGA GAGAACTCCA TGAAGAGGCT 
GTGAGGAGGC AAAAGTTGCT TGAGCAGAGT ATCCAGTCGG CCCAGGAGAT AGAAAAATCC 
TTGCACTTAA TTCAGGAGTC CCTCTCTTCC ATTGACAAGC AGTTGGCAGC TTATATTGCT 
GACAAAGTGG ATGCAGCTCA GATGCCTCAG GAAGCCCAGA AAATCCAATC AGATTTGACA 
AGTCATGAGA TCAGTTTAGA AGAAATGAAG AAACATAACC AGGGAAAGGA GACTGCCCAA 
AGGGTACTAT CCCAAATTGA TGTGGCACAG AAAAAATTGC AGGATGTTTC CATGAAGTTT 
CGATTATTCC AGAAGCCAGC CAATTTTGAG CAGCGCCTAC AAGAAAGTAA AATGATTTTA 
GATGAAGTGA AGATGCATTT ACCTGCGTTG GAAACAAAGA GTGTGGAACA GGAAGTAGTA 
CAGTCACAGT TAAATCATTG TGTGAACTTG TATAAAAGTC TGAGTGAAGT GAAGTCTGAA 
GTGGAAATGG TAATAAAAAC TGGACGTCAG ATTGTACAGA AGAAGCAGAC GGAAAACCCG 
AAAGAGCTTG ATGAAAGAGT TACAGCTTTG AAATTGCATT ATAATGAGCT GGGAGCAAAG 
GTGACAGAAA GAAAGCAACA GTTGGAAAAA TGCTTGAAAT TGTCCCGTAA GATGCGAAAG 
GAAATGAATG CCCTGACAGA ATGGCTGGCA GCGACAGATA TGGAACTGAC GAAGAGATCG 
GCAGTTGAAG GAATGCCTAG TAATTTGGAT TCTGAAGTTG CCTGGGGAAA GGCTACTCAG 
AAAGAGATTG AGAAACAGAA GGTTCACCTA AAGAGTGTCA CAGAGGTAGG AGAGGCCTTG 
AAAACGGTTT TGGGCAAGAA GGAAATGTTG GTGGAAGATA AACTGAGTCT TCTGAATAGT 
AACTGGATAG CCGTCACTTC CCGAGCAGAA GAGTGGTTAA ACCTTTTATT GGAATACCAG 
AAACACATGG AAAACTTTGA CCAGAATGTG GATTACATCA CAAACTGGAT CATTCAGGCT 
GATGCACTTT TGGATGAATC TGAGAAAAAG AAACCTCAGC AAAAAGAAGA CATACTTAAG 
CGTTTAAAGG CTGAAATGAA TGACATACGT CCAAAGGTGG ATTCTACACG TGACCAAGCA 
GCAAACCTGA TGGCAAACCG CGGCGACCAC TGCAGGAAAG TAGTAGAGCC CAAAATCTCA 
GAGCTCAACC ATCGATTTGC AGCCATTTCT CACAGAATTA AGACTGGAAA GGCCTCCATT 
CCTTTGAAGG AATTGGAGCA GTTTAACTCA GATATACAAA AATTGCTTGA ACCACTGGAG 
GCTGAAATTC AGCAGGGGGT GAATCTGAAA GAGGAAGACT TCAATAAAGA TATGAGTGAA 
GACAATGAGG GTACTGTAAA AGAATTGTTG CAAAGAGGAG ACAACTTACA ACAAAGAATC 
ACAGATGAGA GAAAGCGAGA GGAAATAAAG ATAAAACAAC AGCTGTTACA GACAAAACAT 
AATGCTCTCA AGGATTTGAG GTCTCAAAGA AGAAAAAAGG CTCTAGAAAT TTCTCACCAG 
TGGTATCAGT ACAAGAGGCA GGCTGATGAT CTCCTGAAAT GCTTGGATGA CATTGAAAAA 
AAATTAGCCA GCCTACCTGA ACCCAGAGAT GAAAGGAAAA TAAAGGAAAT TGATCGTGAA 
TTGCAGAAGA AGAAAGAGGA GCTGAATGCA GTGCGTAGGC AAGCTGAGGG CTTGTCTGAG 
GATGGGGCCG CAATGGCAGT GGAGCCAACT CAGATCCAGC TCAGCAAGCG CTGGCGGGAA 
ATTGAGAGCA AATTTGCTCA GTTTCGAAGA CTCAACTTTG CACAAATTCA CACTGTCCAT 
GAAGAGTCAG TGGTGGCGAT GACTGAAGAC ATGCCTTTGG AAATTTCTTA TGTGCCTTCT 
ACTTACCTGA CTGAGATCAC TCATGTCTCA CAAGCCCTAT CAGAAGTGGA AGAGCTTCTT 
AATGCTCCCG ACCTTTGTGC TCAAGATTTT GAAGATCTCT TTAAACAAGA GGAATCCTTG 
AAGAACATAA AAGACAGCCT GCAACAAATC TCAGGTCGGA TTGACATCAT TCACAATAAA 
AAGACAGCAG CATTGCACAG TGCCACTCCT GCAGAAAGGG CAAAGCTCCA GGAAGCTCTC 
TCACGGCTTG ATTTCCAATG GGAAAGAGTT AACAATATGT ACAAGGACCG ACAAGGGAGA 
TTTGACAGAT CTGTGGAAAA ATGGCGGCGG TTTCATTATG ATATGAAGAT ACTTAATCAA 
TGGCTAACAG AAGCTGAACA GTTTCTCAAA AAGACACAAA TTCCTGAGAA TTGGGAACAT 
GCCAAATACA AATGGTATCT TAAGGAACTC CAGGATGGCA TTGGACAGCG GCAAAGTGTT 
GTCAGGGTAT TGAATGCAAC TGGGGAAGAA ATAATTCAAC AGTCCTCAAA AACAGATGCC 
AGTATTCTCC AAGAAAAACT GGGAAGCCTG AATCTGCGGT GGCAGGAGGT CTGCAAACAG 
CTGGCAGAAA GAAAAAAGAG GCTAGAGGAA CAGAAGAATA TCTTGTCAGA ATTTCAAAGA 
GATGTAAATG AATTTGTTTT ATGGTTGGAA GAAGCGGATA ACGTTGCTAA TATTCCACTT 
GAACCTGGAA ATGAGCAGCA GCTAAAAGAA AAACTTGAAC AAGTCAAGTT ACTGGCAGAA 
GAGTTGCCCC TGCGCCAGGG AATTCTAAAA CAATTAAATG AAACTGGAGG AACAGTGCTT 
GTAAGTGCTC CCCTAAGCCC AGAAGAGCAA GATAAACTTG AAAATAAGCT CAAGCAGACA 
AATCTTCAGT GGATAAAGGT TTCTAGAAAT CTGCCTGAGA AGCAAGAAGA AATTGAGGCA 
CACGTAAAAG ACCTTGGACA GCTGGAAGAG CAGTTAAATC ATCTGCTTCT ATGGCTGTCT 
CCTATTAGGA ATCAGTTGGA AATTTACAAT CAGCCAAATC AAACAGGACC ATTTGACATC 
AAGGAAATTG AAGTAGCAGT TCAAGCTAAA CAGCCGGATG TGGAAGGGAT TTTGTCTAAA 
GGGCAGCATT TGTACAAGGA AAAACCAGCC ACTCAGCCAG CGAAGAGAAA GCTGGAAGAT 
CTCAGCTCTG ATTGGAAGGT GGTAACTCAG TTGCTTCAAG AGCTGCGGGC AAAGCAGCCT 
GGCCCAGCTC CTGGACTGAC CACTGTCAGA GCCCCTCCCA GTCAGACTGT TACTCTGGTG 
ACACAACCCG CGGTTACCAA GGAAACTGCC ATCTCCAAAC TAGAAATGCC ATCTTCATTG 
CTGTTGGAGG TACCTGCACT GGCAGATTTC AACCGAGCTT GGACAGAACT TACCGACTGG 
CTGTCTCTGC TTGATCGAGT TATAAAATCA CAGAGGGTGA TGGTGGGTGA TCTTGAAGAC 
ATTAACGAGA TGATCATCAA GCAGAAGGCA ACGCTGCAGG ATTTGGAACA GAGGCGCCCC 
CAGTTGGAAG AACTCATTAC CGCTGCCCAG AATTTGAAAA ACAAGACCAG CAATCAAGAG 
GCTAGAACAA TCATTACTGA TCGAATTGAA AGAATTCAGA GTCAGTGGGA TGAAGTACAG 
GAACATCTTC AGAACCGGAG GCAACAGTTG AATGAAATGT TAAAGGATTC CACACAATGG 
CTGGAAGCTA AAGAGGAGGC TGAGCAGGTG TTGGGGCAGG CCAGAGCCAA GCTTGAGTCA 
TGGAAGGAGG CTCCCTACAC AGTAGATGCA ATCCAAAAGA AAATCACAGA AACCAAGCAG 
TTGGCCAAAG ACCTCCGCCA GTGGCAGATA AATGTAGATG TTGCAAATGA TTTGGCACTG 
AAACTTCTCC GAGATTATTC TGCAGATGAT ACCAGAAAAG TACACATGAT AACAGAGAAC 
ATCAATGCCT CTTGGGCAAG CATCCATAAA AGATTGAGTG AGCGAGAGGC TGCTCTGGAA 
GAAACCCACA GATTACTGCA ACAGTTCCCC TTGGACCTGG AGAAGTTCCT TGCCTGGCTT 
ACAGAAGCCG AAACAACTGC CAACGTCCTG CAGGATGCCA CCCATAAGGA AAGGCTTCTA 
GAAGATTCCA AGGGAGTAAG AGAGCTGATG AAACAATGGC AAGATCTCCA AGGAGAAATC 
GAAGCTCACA CAGATATCTA TCACAACCTG GACGAAAATG GCCAAAAAGT CCTGAGATCC 
CTGGAAGGTT CTGACGATGC AGCCTTGTTG CAAAGACGTT TGGATAACAT GAACTTCAAG 
TGGAGCGAAC TTCGGAAAAA GTCTCTCAAC ATTAGGTCTC ACTTGGAAGC CAGTTCTGAC 
CAGTGGAAGC GTCTGCACCT TTCTCTTCAG GAACTTCTGG TATGGCTCCA GCTGAAAGAT 
GATGAGTTAA GCCGGCAGGC ACCCATTGGA GGAGACTTTC CAGCGGTGCA GAAGCAGAAT 
GATGTACACA GGGCCTTCAA GAGGGAATTG AAAACGAAAG AACCTGTAAT CATGAGTACT 
CTTGAGACTG TACGAATATT TCTGACAGAG CAGCCTTTAG AAGGACTAGA GAAACTCTAC 
CAGGAGCCCA GAGAGCTGCC TCCTGAAGAG AGAGCCCAGA ATGTCACACG GCTCCTACGA 
AAGCAAGCTG AGGAGGTCAA CACTCAGTGG GAAAAACTGA ACGTGCACTC TGCAGACTGG 
CAGAGAAAAA TAGACGAGGC CCTCGAAAGA CTCCAGGAGC TTCAGGAAGC AACAGATGAG 
CTGGATCTCA AACTACGTCA GGCAGAGGTG ATCAAAGGAT CCTGGCAGCC TGTGGGTGAT 
CTCCTCATTG ACTCTCTCCA AGATCACCTC GAAAAAGTCA AGGCGCTTCG AGGAGAAATT 
ACACCTCTGA AAGAGAATGT CAGCTACGTC AATGACCTTG CTCGCCAACT CACTACGTTG 
GGCATTCAGC TGTCACCATA TAACCTCAAC ACTCTGGAAG ACCTGAACAC CAGATGGAAG 
CTTCTGCAGG TGGCCATTGA GGACCGCATC AGGCAGCTGC ATGAAGCGCA CAGGGACTTT 
GGACCAGCCT CCCAGCACTT CCTTTCCACT TCTGTCCAGG GTCCCTGGGA GAGAGCCATC 
TCACCAAACA AAGTGCCCTA CTATATCAAC CACGAGACCC AAACAACTTG CTGGGACCAT 
CCCAAAATGA CAGAGCTCTA CCAGTCTTTA GCTGACCTGA ATAATGTCAG GTTCTCAGCT 
TACAGGACTG CCATGAAACT CCGAAGACTG CAGAAGGCCC TTTGCTTGGA TCTCTTGAGC 
CTATCGGCTG CATGCGATGC CTTGGACCAG CACAACCTCA AGCAAAATGA CCAGCCCATG 
GATATCCTGC AGGTCATTAA CTGTCTGACC ACTATTTATG ATCGCCTAGA GCAAGAGCAC 
AACAATCTGG TCAACGTCCC TCTCTGCGTG GATATGTGTC TCAATTGGCT GCTGAATGTT 
TATGACACGG GACGAACGGG GAGGATCCGG GTCCTGTCTT TTAAAACTGG CATCATTTCT 
CTGTGTAAAG CCCATTTGGA AGACAAGTAC AGATACCTCT TCAAGCAAGT GGCAAGTTCG 
ACAGGATTTT GTGACCAGCG CAGGCTGGGC CTCCTCCTGC ATGACTCTAT CCAGATCCCA 
AGACAGTTGG GTGAAGTCGC ATCGTTCGGG GGCAGTAACA TTGAGCCGAG TGTCAGGAGC 
TGCTTCCAGT TTGCTAATAA TAAGCCTGAG ATCGAAGCGG CCCTCTTCCT AGACTGGATG 
CGCCTGGAGC CCCAGTCCAT GGTGTGGCTG CCTGTCCTGC ACCGAGTGGC TGCCGCGGAA 
ACTGCCAAGC ACCAGGCCAA GTGCAACATC TGCAAGGAGT GTCCCATCAT CGGATTCAGG 
TACAGGAGTC TAAAGCACTT TAATTATGAC ATCTGCCAAA GTTGCTTTTT TTCTGGTCGA 
GTTGCAAAAG GCCATAAAAT GCACTATCCC ATGGTGGAAT ACTGCACTCC GACTACATCG 
GGAGAAGATG TCCGTGACTT TGCCAAGGTA CTAAAAAACA AATTTCGAAC CAAAAGGTAT 
TTTGCGAAGC ATCCCCGAAT GGGCTACCTG CCAGTGCAGA CTGTCTTAGA GGGGGACAAC 
ATGGAAACGC CTGCCTCGTC CCCTCAGCTT TCACACGATG ATACTCATTC ACGCATTGAG 
CATTATGCTA GCAGGCTAGC AGAAATGGAA AACAGCAATG GATCTTATCT AAATGATAGC 
ATCTCTCCTA ATGAGAGCAT AGATGATGAA CATTTGTTAA TCCAGCATTA CTGCCAAAGT 
TTGAACCAGG ACTCCCCCCT GAGCCAGCCT CGTAGTCCTG CCCAGATCTT GATTTCCTTA 
GAGAGTGAGG AAAGAGGGGA GCTAGAGAGA ATCCTAGCAG ATCTTGAGGA AGAAAACAGA 
AATCTGCAAG CAGAGTATGA TCGTCTAAAG CAGCAGCATG AACACAAAGG CCTGTCCCCA 
CTGCCATCCC CTCCTGAAAT GATGCCTACT TCTCCCCAAA GTCCCCGGGA TGCTGAGCTC 
ATCGCTGAGG CCAAGCTGCT GCGTCAACAC AAAGGCCGCC TGGAAGCCAG GATGCAAATC 
CTTGAAGACC ATAACAAACA ACTGGAATCC CAGTTACACA GGCTCAGGCA GCTGCTGGAG 
CAACCCCAGG CAGAGGCCAA GGTGAATGGT ACAACGGTGT CTTCTCCTTC TACCTCTCTT 
CAGAGGTCAG ATAGCAGTCA GCCTATGCTG CTCCGGGTGG TCGGCAGTCA GACTTCAGAA 
TCCATGGGCG AGGAAGACCT GCTCAGCCCT CCCCAGGACA CAAGCACAGG GTTAGAGGAA 
GTGATGGAGC AGCTCAACCA CTCCTTCCCT AGTTCCAGAG GACACAATGT AGGAAGCCTT 
TTCCACTTGG CAGATGATTT GGGCAGAGCG ATGGAGTCCT TAGTTTCAGT CATGACAGAT 
GAAGAAGGGG CAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMD ( XM_005641032.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005641089.1 Canis lupus familiaris dystrophin (DMD), transcript variant X9, mRNA.

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CloneID	OCe31976
Clone ID Related Accession (Same CDS sequence)	XM_005641032.2
Accession Version	XM_005641032.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	dystrophin isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005641032.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTGGT GGGAAGAAGT AGAGGACTGT TATGAAAGAG AAGATGTTCA AAAGAAAACA 
TTCACAAAAT GGATAAATGC ACAGTTTTCT AAGTTTGGGA AGCAGCACAT AGAGAACCTC 
TTCAGTGACC TACAGGATGG GAGACGCCTC CTAGACCTTT TGGAAGGCCT GACAGGGCAA 
AAACTGCCAA AAGAAAAAGG ATCCACAAGA GTTCATGCCC TGAACAATGT CAACAAGGCA 
CTGCGCGTCT TGCAGAAAAA TAATGTTGAT TTAGTGAACA TTGGAAGTAC TGACATAGTA 
GATGGAAATC ACAAACTGAC TCTTGGTTTG ATTTGGAATA TAATCCTCCA CTGGCAGGTC 
AAAAATGTAA TGAAAAATAT CATGGCTGGA TTGCAACAAA CCAACAGTGA AAAGATTCTC 
CTGAGCTGGG TCCGACAATC AACTCGTAAT TATCCACAGG TTAATGTCAT TAACTTCACC 
ACCAGCTGGT CTGATGGCCT GGCTTTGAAC GCTCTCATCC ACAGTCATAG GCCAGACCTG 
TTTGATTGGA ATAGTGTGGT TTGCCAGCAG TCAGCCACAC AACGCCTGGA ACATGCATTC 
AACATTGCCA AATATCAATT AGGCATAGAG AAACTGCTTG ATCCTGAAGA TGTTGCCACC 
ACTTATCCAG ATAAGAAGTC CATCTTAATG TATATCACAT CACTCTTCCA AGTTTTGCCT 
CAACAAGTGA GCATTGAAGC CATCCAGGAA GTGGAAATGT TGCCAAGGCC ATCAAAAGTT 
ACTAGAGAAG AACATTTTCA ATTACATCAT CAAATGCACT ATTCTCAACA GATCACAGTC 
AGTCTAGCAC AGGGCTATGA ACGAGCCCCT TCCTCTCCTA AGCCTCGGTT CAAGAGCTAT 
GCCTACACAC AGGCTGCTTA TGTCACCACT TCTGACCCCA CACGGAGCCC ACTTCCTTCA 
CAGCATTTGG AAACTCCTGA AGACAAGTCA TTTGGCCGGT CATTGACAGA GACCGAAGCA 
AACCTGGACA GTTATCAAAC AGCTTTGGAA GAAGTACTCT CGTGGCTTCT TTCAGCTGAG 
GATGCACTGC AAGCCCAAGG AGAGATTTCT AATGATGTCG AAGAAGTGAA AGAACAATTT 
CATACTCATG AGGGATATAT GATGGACTTG ACATCCCATC AGGGACGGGT CGGTAATGTT 
CTCCAACTGG GAAGTCAACT GATTGGAACA GGGAAATTAT CAGAAGATGA AGAAACCGAA 
GTGCAGGAAC AAATGAATCT CCTCAATTCA AGATGGGAAT GCCTCAGGGT AGCTAGCATG 
GAAAAACAAA GCAATTTACA TAAAGTTCTA ATGGATCTCC AGAATCAGCA ACTGAAAGAG 
TTAAATGACT GGCTAACCAA AACAGAAGAG AGAACAAGGA AAATGGAGAA GGAGCCCCTT 
GGACCTGATA TTGAAGACCT AAAACGCCAA GTACAACAAC ATAAGGTGCT TCAAGAAGAC 
TTAGAACAGG AACAAGTCAG GGTCAATTCC CTCACTCATA TGGTGGTGGT AGTCGATGAA 
TCTAGTGGAG ACCATGCAAC TGCTGCTTTG GAAGAACAAC TTAAGGTACT GGGAGATCGA 
TGGGCAAACA TCTGTAGGTG GACAGAAGAT CGCTGGGTTC TTTTACAAGA CATCCTCCTA 
AAATGGCAGC GTTTTACTGA AGAACAGTGC CTTTTCAGTG CATGGCTTTC GGAGAAGGAA 
GATGCAGTGA ACAAGATTCA CACAACTGGC TTTAAGGATC AAAGTGAAGT GTTATCAAAT 
CTTCAGAAAC TGGCTGTCTT AAAAACAGAT CTGGAAAAGA AGAAGCAAAC CATGGACAAA 
CTCTGCTCAC TCAACCAAGA CCTTCTTTCA GCGCTGAAAA ACACAGTGGT AGCCCACAAG 
ATGGAAGCAT GGCTGGACAA CTTTGCCCAG CGCTGGGATA ATTTAGTCCA GAAACTTGAA 
AAAAGTTCAG CACAGATTTC ACAGGCTGTC ACCACCACTC AGCCATCACT AACACAGACA 
ACTGTAATGG AAACAGTAAC TATGGTGACC ACGAGGGAAC ACATCTTGGT AAAGCATGCC 
CAAGAGGAAC TGCCACCACC ACCCCCTCAG AAGAAGAGGC AGATTATCGT GGATTCTGAA 
ATTAGGAAAA GGTTGGATGT CGATATAACT GAACTTCACA GTTGGATTAC TCGTTCAGAA 
GCTGTGTTGC AGAGTCCTGA ATTTGCAATC TATCGGAAGG AAGGCAACTT CTCAGACCTT 
AAAGAAAAAG TCAATGCCAT AGAGCGAGAA AAAGCCGAGA AGTTCAGAAA ACTGCAAGAT 
GCCAGCAGAT CAGCTCAGGC CCTGGTGGAA CAGATGGTGA ATGAGGGTGT TAATGCTGAC 
AGCATCAAAC AAGCCTCCGA ACAACTGAAC AGCCGGTGGA TAGAGTTCTG CCAATTGCTA 
AGCGAGAGAC TTAACTGGCT GGAGTATCAG AACAACATCA TCACTTTCTA TAATCAGCTA 
CAACAATTGG AGCAGATGAC AACTACTGCT GAAAACTGGT TGAAAACCCA GCCTACCACC 
ACATCAGAGC CAACAGCAAT TAAAAGCCAG TTAAAAATTT GTAAGGATGA AATCAACCGA 
CTGTCAGCTC TTCAGCCTCA AATCGAGCGA TTAAAAATTC AAAGCATAGC CCTGAAAGAG 
AAAGGACAAG GGCCAATGTT CCTGGATGCA GACTTTGTGG CCTTTACAAA TCATTTTAAC 
CAAGTCTTTG CTGATGTGCA GGCAAGAGAA AAAGAGCTAC AAACAATTTT TGACAGTTTG 
CCACCCATGC GCTATCAGGA GACTATGAGT ACCATCCTGA CATGGATCCA GCAGTCAGAA 
ACCAAACTCT CTATACCTCA GGTTACTGTC ACTGAATATG ACATCATGGA ACAGAGACTC 
GGAGAGCTAC AGGCTTTACA AAGCTCTCTG CAAGAGCAAC AAAATGGCCT AAACTATCTC 
AGCACCACTG TGAAAGAGAT GTCAAAGAAA GCACCACTGT CTGATATTAG TCGGAAATAT 
CAATCAGAAT TTGAAGAGAT TGAGGGACGT TGGAAGAAGC TGTCTTCCCA GCTGGTTGAA 
CATTGTCAAA AGTTGGAGGA GCAAATGGCT AAACTTCGAA AAATTCAGAA TCACATAAAA 
ACTCTGAAGA AATGGATCAC TGAAGTCGAT GTTTTCCTGA AGGAGGAATG GCCTGCCCTT 
GGGGATTCAG AAATTCTGAA AAGACAGCTG AAACAGTGCA GGCTTTTAGT CAATGACATT 
CAGACCATCC AGCCTAGTCT CAACAGTGTC AATGAAGGGG CTCAGAAGAT GAAGAATGAA 
GCAGAACCAG AGTTTGCTGG CAGACTTGAG ACAGAGCTCC GAGAACTTAA CACCCAGTGG 
GATTACATGT GCCGCCAGGT CTATGCCAGG AAGGAAGCCT TAAAAGGAGG TTTGGATAAA 
ACTGTAAGTC TTCAGAAAGA TCTGTCAGAG ATGCATGAGT GGATGACACA AGCTGAAGAA 
GAATACCTAG AGAGAGATTT CGAATACAAG ACCCCTGATG AATTACAGAC AGCAGTTGAA 
GAGATGAAGA GAGCTAAAGA AGAGGCCCAG CAAAAAGAAG CAAAAGTGAA ACTCCTAACC 
GAGTCCGTCA ATAGTGTCAT AGCTCAGGCT CCACCTGCAG CACAAGAGGC CTTAAAAAAG 
GAACTTGACA CTCTCACCAC CAACTACCAG TGGCTCTGCA CCAGGCTCAA TGGCAAATGC 
AAGACCTTGG AAGAAGTTTG GGCGTGCTGG CATGAGTTAT TGTCCTACTT GGAGAAGGCA 
AACAAGTGGC TAAGTGAAGT AGAAGTCAAG CTTAAAACCA CTGAAAATAT TTCTGGGGGA 
GCTGAGGAAA TCGCCGAGGT GCTTGATTCG CTTGAAAATT TGATGCAACA TTCAGAGGAT 
AACCCGAATC AGATTCGCAT ATTGGCACAG ACCTTGACAG ATGGTGGAGT CATGGATGAA 
CTGATCAATG AGGAGCTTGA GACATTTAAT TCTCGTTGGA GAGAACTCCA TGAAGAGGCT 
GTGAGGAGGC AAAAGTTGCT TGAGCAGAGT ATCCAGTCGG CCCAGGAGAT AGAAAAATCC 
TTGCACTTAA TTCAGGAGTC CCTCTCTTCC ATTGACAAGC AGTTGGCAGC TTATATTGCT 
GACAAAGTGG ATGCAGCTCA GATGCCTCAG GAAGCCCAGA AAATCCAATC AGATTTGACA 
AGTCATGAGA TCAGTTTAGA AGAAATGAAG AAACATAACC AGGGAAAGGA GACTGCCCAA 
AGGGTACTAT CCCAAATTGA TGTGGCACAG AAAAAATTGC AGGATGTTTC CATGAAGTTT 
CGATTATTCC AGAAGCCAGC CAATTTTGAG CAGCGCCTAC AAGAAAGTAA AATGATTTTA 
GATGAAGTGA AGATGCATTT ACCTGCGTTG GAAACAAAGA GTGTGGAACA GGAAGTAGTA 
CAGTCACAGT TAAATCATTG TGTGAACTTG TATAAAAGTC TGAGTGAAGT GAAGTCTGAA 
GTGGAAATGG TAATAAAAAC TGGACGTCAG ATTGTACAGA AGAAGCAGAC GGAAAACCCG 
AAAGAGCTTG ATGAAAGAGT TACAGCTTTG AAATTGCATT ATAATGAGCT GGGAGCAAAG 
GTGACAGAAA GAAAGCAACA GTTGGAAAAA TGCTTGAAAT TGTCCCGTAA GATGCGAAAG 
GAAATGAATG CCCTGACAGA ATGGCTGGCA GCGACAGATA TGGAACTGAC GAAGAGATCG 
GCAGTTGAAG GAATGCCTAG TAATTTGGAT TCTGAAGTTG CCTGGGGAAA GGCTACTCAG 
AAAGAGATTG AGAAACAGAA GGTTCACCTA AAGAGTGTCA CAGAGGTAGG AGAGGCCTTG 
AAAACGGTTT TGGGCAAGAA GGAAATGTTG GTGGAAGATA AACTGAGTCT TCTGAATAGT 
AACTGGATAG CCGTCACTTC CCGAGCAGAA GAGTGGTTAA ACCTTTTATT GGAATACCAG 
AAACACATGG AAAACTTTGA CCAGAATGTG GATTACATCA CAAACTGGAT CATTCAGGCT 
GATGCACTTT TGGATGAATC TGAGAAAAAG AAACCTCAGC AAAAAGAAGA CATACTTAAG 
CGTTTAAAGG CTGAAATGAA TGACATACGT CCAAAGGTGG ATTCTACACG TGACCAAGCA 
GCAAACCTGA TGGCAAACCG CGGCGACCAC TGCAGGAAAG TAGTAGAGCC CAAAATCTCA 
GAGCTCAACC ATCGATTTGC AGCCATTTCT CACAGAATTA AGACTGGAAA GGCCTCCATT 
CCTTTGAAGG AATTGGAGCA GTTTAACTCA GATATACAAA AATTGCTTGA ACCACTGGAG 
GCTGAAATTC AGCAGGGGGT GAATCTGAAA GAGGAAGACT TCAATAAAGA TATGAGTGAA 
GACAATGAGG GTACTGTAAA AGAATTGTTG CAAAGAGGAG ACAACTTACA ACAAAGAATC 
ACAGATGAGA GAAAGCGAGA GGAAATAAAG ATAAAACAAC AGCTGTTACA GACAAAACAT 
AATGCTCTCA AGGATTTGAG GTCTCAAAGA AGAAAAAAGG CTCTAGAAAT TTCTCACCAG 
TGGTATCAGT ACAAGAGGCA GGCTGATGAT CTCCTGAAAT GCTTGGATGA CATTGAAAAA 
AAATTAGCCA GCCTACCTGA ACCCAGAGAT GAAAGGAAAA TAAAGGAAAT TGATCGTGAA 
TTGCAGAAGA AGAAAGAGGA GCTGAATGCA GTGCGTAGGC AAGCTGAGGG CTTGTCTGAG 
GATGGGGCCG CAATGGCAGT GGAGCCAACT CAGATCCAGC TCAGCAAGCG CTGGCGGGAA 
ATTGAGAGCA AATTTGCTCA GTTTCGAAGA CTCAACTTTG CACAAATTCA CACTGTCCAT 
GAAGAGTCAG TGGTGGCGAT GACTGAAGAC ATGCCTTTGG AAATTTCTTA TGTGCCTTCT 
ACTTACCTGA CTGAGATCAC TCATGTCTCA CAAGCCCTAT CAGAAGTGGA AGAGCTTCTT 
AATGCTCCCG ACCTTTGTGC TCAAGATTTT GAAGATCTCT TTAAACAAGA GGAATCCTTG 
AAGAACATAA AAGACAGCCT GCAACAAATC TCAGGTCGGA TTGACATCAT TCACAATAAA 
AAGACAGCAG CATTGCACAG TGCCACTCCT GCAGAAAGGG CAAAGCTCCA GGAAGCTCTC 
TCACGGCTTG ATTTCCAATG GGAAAGAGTT AACAATATGT ACAAGGACCG ACAAGGGAGA 
TTTGACAGAT CTGTGGAAAA ATGGCGGCGG TTTCATTATG ATATGAAGAT ACTTAATCAA 
TGGCTAACAG AAGCTGAACA GTTTCTCAAA AAGACACAAA TTCCTGAGAA TTGGGAACAT 
GCCAAATACA AATGGTATCT TAAGGAACTC CAGGATGGCA TTGGACAGCG GCAAAGTGTT 
GTCAGGGTAT TGAATGCAAC TGGGGAAGAA ATAATTCAAC AGTCCTCAAA AACAGATGCC 
AGTATTCTCC AAGAAAAACT GGGAAGCCTG AATCTGCGGT GGCAGGAGGT CTGCAAACAG 
CTGGCAGAAA GAAAAAAGAG GCTAGAGGAA CAGAAGAATA TCTTGTCAGA ATTTCAAAGA 
GATGTAAATG AATTTGTTTT ATGGTTGGAA GAAGCGGATA ACGTTGCTAA TATTCCACTT 
GAACCTGGAA ATGAGCAGCA GCTAAAAGAA AAACTTGAAC AAGTCAAGTT ACTGGCAGAA 
GAGTTGCCCC TGCGCCAGGG AATTCTAAAA CAATTAAATG AAACTGGAGG AACAGTGCTT 
GTAAGTGCTC CCCTAAGCCC AGAAGAGCAA GATAAACTTG AAAATAAGCT CAAGCAGACA 
AATCTTCAGT GGATAAAGGT TTCTAGAAAT CTGCCTGAGA AGCAAGAAGA AATTGAGGCA 
CACGTAAAAG ACCTTGGACA GCTGGAAGAG CAGTTAAATC ATCTGCTTCT ATGGCTGTCT 
CCTATTAGGA ATCAGTTGGA AATTTACAAT CAGCCAAATC AAACAGGACC ATTTGACATC 
AAGGAAATTG AAGTAGCAGT TCAAGCTAAA CAGCCGGATG TGGAAGGGAT TTTGTCTAAA 
GGGCAGCATT TGTACAAGGA AAAACCAGCC ACTCAGCCAG CGAAGAGAAA GCTGGAAGAT 
CTCAGCTCTG ATTGGAAGGT GGTAACTCAG TTGCTTCAAG AGCTGCGGGC AAAGCAGCCT 
GGCCCAGCTC CTGGACTGAC CACTGTCAGA GCCCCTCCCA GTCAGACTGT TACTCTGGTG 
ACACAACCCG CGGTTACCAA GGAAACTGCC ATCTCCAAAC TAGAAATGCC ATCTTCATTG 
CTGTTGGAGG TACCTGCACT GGCAGATTTC AACCGAGCTT GGACAGAACT TACCGACTGG 
CTGTCTCTGC TTGATCGAGT TATAAAATCA CAGAGGGTGA TGGTGGGTGA TCTTGAAGAC 
ATTAACGAGA TGATCATCAA GCAGAAGGCA ACGCTGCAGG ATTTGGAACA GAGGCGCCCC 
CAGTTGGAAG AACTCATTAC CGCTGCCCAG AATTTGAAAA ACAAGACCAG CAATCAAGAG 
GCTAGAACAA TCATTACTGA TCGAATTGAA AGAATTCAGA GTCAGTGGGA TGAAGTACAG 
GAACATCTTC AGAACCGGAG GCAACAGTTG AATGAAATGT TAAAGGATTC CACACAATGG 
CTGGAAGCTA AAGAGGAGGC TGAGCAGGTG TTGGGGCAGG CCAGAGCCAA GCTTGAGTCA 
TGGAAGGAGG CTCCCTACAC AGTAGATGCA ATCCAAAAGA AAATCACAGA AACCAAGCAG 
TTGGCCAAAG ACCTCCGCCA GTGGCAGATA AATGTAGATG TTGCAAATGA TTTGGCACTG 
AAACTTCTCC GAGATTATTC TGCAGATGAT ACCAGAAAAG TACACATGAT AACAGAGAAC 
ATCAATGCCT CTTGGGCAAG CATCCATAAA AGATTGAGTG AGCGAGAGGC TGCTCTGGAA 
GAAACCCACA GATTACTGCA ACAGTTCCCC TTGGACCTGG AGAAGTTCCT TGCCTGGCTT 
ACAGAAGCCG AAACAACTGC CAACGTCCTG CAGGATGCCA CCCATAAGGA AAGGCTTCTA 
GAAGATTCCA AGGGAGTAAG AGAGCTGATG AAACAATGGC AAGATCTCCA AGGAGAAATC 
GAAGCTCACA CAGATATCTA TCACAACCTG GACGAAAATG GCCAAAAAGT CCTGAGATCC 
CTGGAAGGTT CTGACGATGC AGCCTTGTTG CAAAGACGTT TGGATAACAT GAACTTCAAG 
TGGAGCGAAC TTCGGAAAAA GTCTCTCAAC ATTAGGTCTC ACTTGGAAGC CAGTTCTGAC 
CAGTGGAAGC GTCTGCACCT TTCTCTTCAG GAACTTCTGG TATGGCTCCA GCTGAAAGAT 
GATGAGTTAA GCCGGCAGGC ACCCATTGGA GGAGACTTTC CAGCGGTGCA GAAGCAGAAT 
GATGTACACA GGGCCTTCAA GAGGGAATTG AAAACGAAAG AACCTGTAAT CATGAGTACT 
CTTGAGACTG TACGAATATT TCTGACAGAG CAGCCTTTAG AAGGACTAGA GAAACTCTAC 
CAGGAGCCCA GAGAGCTGCC TCCTGAAGAG AGAGCCCAGA ATGTCACACG GCTCCTACGA 
AAGCAAGCTG AGGAGGTCAA CACTCAGTGG GAAAAACTGA ACGTGCACTC TGCAGACTGG 
CAGAGAAAAA TAGACGAGGC CCTCGAAAGA CTCCAGGAGC TTCAGGAAGC AACAGATGAG 
CTGGATCTCA AACTACGTCA GGCAGAGGTG ATCAAAGGAT CCTGGCAGCC TGTGGGTGAT 
CTCCTCATTG ACTCTCTCCA AGATCACCTC GAAAAAGTCA AGGCGCTTCG AGGAGAAATT 
ACACCTCTGA AAGAGAATGT CAGCTACGTC AATGACCTTG CTCGCCAACT CACTACGTTG 
GGCATTCAGC TGTCACCATA TAACCTCAAC ACTCTGGAAG ACCTGAACAC CAGATGGAAG 
CTTCTGCAGG TGGCCATTGA GGACCGCATC AGGCAGCTGC ATGAAGCGCA CAGGGACTTT 
GGACCAGCCT CCCAGCACTT CCTTTCCACT TCTGTCCAGG GTCCCTGGGA GAGAGCCATC 
TCACCAAACA AAGTGCCCTA CTATATCAAC CACGAGACCC AAACAACTTG CTGGGACCAT 
CCCAAAATGA CAGAGCTCTA CCAGTCTTTA GCTGACCTGA ATAATGTCAG GTTCTCAGCT 
TACAGGACTG CCATGAAACT CCGAAGACTG CAGAAGGCCC TTTGCTTGGA TCTCTTGAGC 
CTATCGGCTG CATGCGATGC CTTGGACCAG CACAACCTCA AGCAAAATGA CCAGCCCATG 
GATATCCTGC AGGTCATTAA CTGTCTGACC ACTATTTATG ATCGCCTAGA GCAAGAGCAC 
AACAATCTGG TCAACGTCCC TCTCTGCGTG GATATGTGTC TCAATTGGCT GCTGAATGTT 
TATGACACGG GACGAACGGG GAGGATCCGG GTCCTGTCTT TTAAAACTGG CATCATTTCT 
CTGTGTAAAG CCCATTTGGA AGACAAGTAC AGATACCTCT TCAAGCAAGT GGCAAGTTCG 
ACAGGATTTT GTGACCAGCG CAGGCTGGGC CTCCTCCTGC ATGACTCTAT CCAGATCCCA 
AGACAGTTGG GTGAAGTCGC ATCGTTCGGG GGCAGTAACA TTGAGCCGAG TGTCAGGAGC 
TGCTTCCAGT TTGCTAATAA TAAGCCTGAG ATCGAAGCGG CCCTCTTCCT AGACTGGATG 
CGCCTGGAGC CCCAGTCCAT GGTGTGGCTG CCTGTCCTGC ACCGAGTGGC TGCCGCGGAA 
ACTGCCAAGC ACCAGGCCAA GTGCAACATC TGCAAGGAGT GTCCCATCAT CGGATTCAGG 
TACAGGAGTC TAAAGCACTT TAATTATGAC ATCTGCCAAA GTTGCTTTTT TTCTGGTCGA 
GTTGCAAAAG GCCATAAAAT GCACTATCCC ATGGTGGAAT ACTGCACTCC GACTACATCG 
GGAGAAGATG TCCGTGACTT TGCCAAGGTA CTAAAAAACA AATTTCGAAC CAAAAGGTAT 
TTTGCGAAGC ATCCCCGAAT GGGCTACCTG CCAGTGCAGA CTGTCTTAGA GGGGGACAAC 
ATGGAAACAA ATCTGCAAGC AGAGTATGAT CGTCTAAAGC AGCAGCATGA ACACAAAGGC 
CTGTCCCCAC TGCCATCCCC TCCTGAAATG ATGCCTACTT CTCCCCAAAG TCCCCGGGAT 
GCTGAGCTCA TCGCTGAGGC CAAGCTGCTG CGTCAACACA AAGGCCGCCT GGAAGCCAGG 
ATGCAAATCC TTGAAGACCA TAACAAACAA CTGGAATCCC AGTTACACAG GCTCAGGCAG 
CTGCTGGAGC AACCCCAGGC AGAGGCCAAG GTGAATGGTA CAACGGTGTC TTCTCCTTCT 
ACCTCTCTTC AGAGGTCAGA TAGCAGTCAG CCTATGCTGC TCCGGGTGGT CGGCAGTCAG 
ACTTCAGAAT CCATGGGCGA GGAAGACCTG CTCAGCCCTC CCCAGGACAC AAGCACAGGG 
TTAGAGGAAG TGATGGAGCA GCTCAACCAC TCCTTCCCTA GTTCCAGAGG ACACAATGTA 
GGAAGCCTTT TCCACTTGGC AGATGATTTG GGCAGAGCGA TGGAGTCCTT AGTTTCAGTC 
ATGACAGATG AAGAAGGGGC AGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005641089.1
CDS	248..11014
Translation	MLWWEEVEDCYEREDVQKKTFTKWINAQFSKFGKQHIENLFSDLQDGRRLLDLLEGLTGQKLPKEKGSTRVHALNNVNKALRVLQKNNVDLVNIGSTDIVDGNHKLTLGLIWNIILHWQVKNVMKNIMAGLQQTNSEKILLSWVRQSTRNYPQVNVINFTTSWSDGLALNALIHSHRPDLFDWNSVVCQQSATQRLEHAFNIAKYQLGIEKLLDPEDVATTYPDKKSILMYITSLFQVLPQQVSIEAIQEVEMLPRPSKVTREEHFQLHHQMHYSQQITVSLAQGYERAPSSPKPRFKSYAYTQAAYVTTSDPTRSPLPSQHLETPEDKSFGRSLTETEANLDSYQTALEEVLSWLLSAEDALQAQGEISNDVEEVKEQFHTHEGYMMDLTSHQGRVGNVLQLGSQLIGTGKLSEDEETEVQEQMNLLNSRWECLRVASMEKQSNLHKVLMDLQNQQLKELNDWLTKTEERTRKMEKEPLGPDIEDLKRQVQQHKVLQEDLEQEQVRVNSLTHMVVVVDESSGDHATAALEEQLKVLGDRWANICRWTEDRWVLLQDILLKWQRFTEEQCLFSAWLSEKEDAVNKIHTTGFKDQSEVLSNLQKLAVLKTDLEKKKQTMDKLCSLNQDLLSALKNTVVAHKMEAWLDNFAQRWDNLVQKLEKSSAQISQAVTTTQPSLTQTTVMETVTMVTTREHILVKHAQEELPPPPPQKKRQIIVDSEIRKRLDVDITELHSWITRSEAVLQSPEFAIYRKEGNFSDLKEKVNAIEREKAEKFRKLQDASRSAQALVEQMVNEGVNADSIKQASEQLNSRWIEFCQLLSERLNWLEYQNNIITFYNQLQQLEQMTTTAENWLKTQPTTTSEPTAIKSQLKICKDEINRLSALQPQIERLKIQSIALKEKGQGPMFLDADFVAFTNHFNQVFADVQAREKELQTIFDSLPPMRYQETMSTILTWIQQSETKLSIPQVTVTEYDIMEQRLGELQALQSSLQEQQNGLNYLSTTVKEMSKKAPLSDISRKYQSEFEEIEGRWKKLSSQLVEHCQKLEEQMAKLRKIQNHIKTLKKWITEVDVFLKEEWPALGDSEILKRQLKQCRLLVNDIQTIQPSLNSVNEGAQKMKNEAEPEFAGRLETELRELNTQWDYMCRQVYARKEALKGGLDKTVSLQKDLSEMHEWMTQAEEEYLERDFEYKTPDELQTAVEEMKRAKEEAQQKEAKVKLLTESVNSVIAQAPPAAQEALKKELDTLTTNYQWLCTRLNGKCKTLEEVWACWHELLSYLEKANKWLSEVEVKLKTTENISGGAEEIAEVLDSLENLMQHSEDNPNQIRILAQTLTDGGVMDELINEELETFNSRWRELHEEAVRRQKLLEQSIQSAQEIEKSLHLIQESLSSIDKQLAAYIADKVDAAQMPQEAQKIQSDLTSHEISLEEMKKHNQGKETAQRVLSQIDVAQKKLQDVSMKFRLFQKPANFEQRLQESKMILDEVKMHLPALETKSVEQEVVQSQLNHCVNLYKSLSEVKSEVEMVIKTGRQIVQKKQTENPKELDERVTALKLHYNELGAKVTERKQQLEKCLKLSRKMRKEMNALTEWLAATDMELTKRSAVEGMPSNLDSEVAWGKATQKEIEKQKVHLKSVTEVGEALKTVLGKKEMLVEDKLSLLNSNWIAVTSRAEEWLNLLLEYQKHMENFDQNVDYITNWIIQADALLDESEKKKPQQKEDILKRLKAEMNDIRPKVDSTRDQAANLMANRGDHCRKVVEPKISELNHRFAAISHRIKTGKASIPLKELEQFNSDIQKLLEPLEAEIQQGVNLKEEDFNKDMSEDNEGTVKELLQRGDNLQQRITDERKREEIKIKQQLLQTKHNALKDLRSQRRKKALEISHQWYQYKRQADDLLKCLDDIEKKLASLPEPRDERKIKEIDRELQKKKEELNAVRRQAEGLSEDGAAMAVEPTQIQLSKRWREIESKFAQFRRLNFAQIHTVHEESVVAMTEDMPLEISYVPSTYLTEITHVSQALSEVEELLNAPDLCAQDFEDLFKQEESLKNIKDSLQQISGRIDIIHNKKTAALHSATPAERAKLQEALSRLDFQWERVNNMYKDRQGRFDRSVEKWRRFHYDMKILNQWLTEAEQFLKKTQIPENWEHAKYKWYLKELQDGIGQRQSVVRVLNATGEEIIQQSSKTDASILQEKLGSLNLRWQEVCKQLAERKKRLEEQKNILSEFQRDVNEFVLWLEEADNVANIPLEPGNEQQLKEKLEQVKLLAEELPLRQGILKQLNETGGTVLVSAPLSPEEQDKLENKLKQTNLQWIKVSRNLPEKQEEIEAHVKDLGQLEEQLNHLLLWLSPIRNQLEIYNQPNQTGPFDIKEIEVAVQAKQPDVEGILSKGQHLYKEKPATQPAKRKLEDLSSDWKVVTQLLQELRAKQPGPAPGLTTVRAPPSQTVTLVTQPAVTKETAISKLEMPSSLLLEVPALADFNRAWTELTDWLSLLDRVIKSQRVMVGDLEDINEMIIKQKATLQDLEQRRPQLEELITAAQNLKNKTSNQEARTIITDRIERIQSQWDEVQEHLQNRRQQLNEMLKDSTQWLEAKEEAEQVLGQARAKLESWKEAPYTVDAIQKKITETKQLAKDLRQWQINVDVANDLALKLLRDYSADDTRKVHMITENINASWASIHKRLSEREAALEETHRLLQQFPLDLEKFLAWLTEAETTANVLQDATHKERLLEDSKGVRELMKQWQDLQGEIEAHTDIYHNLDENGQKVLRSLEGSDDAALLQRRLDNMNFKWSELRKKSLNIRSHLEASSDQWKRLHLSLQELLVWLQLKDDELSRQAPIGGDFPAVQKQNDVHRAFKRELKTKEPVIMSTLETVRIFLTEQPLEGLEKLYQEPRELPPEERAQNVTRLLRKQAEEVNTQWEKLNVHSADWQRKIDEALERLQELQEATDELDLKLRQAEVIKGSWQPVGDLLIDSLQDHLEKVKALRGEITPLKENVSYVNDLARQLTTLGIQLSPYNLNTLEDLNTRWKLLQVAIEDRIRQLHEAHRDFGPASQHFLSTSVQGPWERAISPNKVPYYINHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQVINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETNLQAEYDRLKQQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDTSTGLEEVMEQLNHSFPSSRGHNVGSLFHLADDLGRAMESLVSVMTDEEGAE

Target ORF information:

RefSeq Version	XM_005641032.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris dystrophin (DMD), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005641032.2

ORF Insert Sequence:

ATGCTTTGGT GGGAAGAAGT AGAGGACTGT TATGAAAGAG AAGATGTTCA AAAGAAAACA 
TTCACAAAAT GGATAAATGC ACAGTTTTCT AAGTTTGGGA AGCAGCACAT AGAGAACCTC 
TTCAGTGACC TACAGGATGG GAGACGCCTC CTAGACCTTT TGGAAGGCCT GACAGGGCAA 
AAACTGCCAA AAGAAAAAGG ATCCACAAGA GTTCATGCCC TGAACAATGT CAACAAGGCA 
CTGCGCGTCT TGCAGAAAAA TAATGTTGAT TTAGTGAACA TTGGAAGTAC TGACATAGTA 
GATGGAAATC ACAAACTGAC TCTTGGTTTG ATTTGGAATA TAATCCTCCA CTGGCAGGTC 
AAAAATGTAA TGAAAAATAT CATGGCTGGA TTGCAACAAA CCAACAGTGA AAAGATTCTC 
CTGAGCTGGG TCCGACAATC AACTCGTAAT TATCCACAGG TTAATGTCAT TAACTTCACC 
ACCAGCTGGT CTGATGGCCT GGCTTTGAAC GCTCTCATCC ACAGTCATAG GCCAGACCTG 
TTTGATTGGA ATAGTGTGGT TTGCCAGCAG TCAGCCACAC AACGCCTGGA ACATGCATTC 
AACATTGCCA AATATCAATT AGGCATAGAG AAACTGCTTG ATCCTGAAGA TGTTGCCACC 
ACTTATCCAG ATAAGAAGTC CATCTTAATG TATATCACAT CACTCTTCCA AGTTTTGCCT 
CAACAAGTGA GCATTGAAGC CATCCAGGAA GTGGAAATGT TGCCAAGGCC ATCAAAAGTT 
ACTAGAGAAG AACATTTTCA ATTACATCAT CAAATGCACT ATTCTCAACA GATCACAGTC 
AGTCTAGCAC AGGGCTATGA ACGAGCCCCT TCCTCTCCTA AGCCTCGGTT CAAGAGCTAT 
GCCTACACAC AGGCTGCTTA TGTCACCACT TCTGACCCCA CACGGAGCCC ACTTCCTTCA 
CAGCATTTGG AAACTCCTGA AGACAAGTCA TTTGGCCGGT CATTGACAGA GACCGAAGCA 
AACCTGGACA GTTATCAAAC AGCTTTGGAA GAAGTACTCT CGTGGCTTCT TTCAGCTGAG 
GATGCACTGC AAGCCCAAGG AGAGATTTCT AATGATGTCG AAGAAGTGAA AGAACAATTT 
CATACTCATG AGGGATATAT GATGGACTTG ACATCCCATC AGGGACGGGT CGGTAATGTT 
CTCCAACTGG GAAGTCAACT GATTGGAACA GGGAAATTAT CAGAAGATGA AGAAACCGAA 
GTGCAGGAAC AAATGAATCT CCTCAATTCA AGATGGGAAT GCCTCAGGGT AGCTAGCATG 
GAAAAACAAA GCAATTTACA TAAAGTTCTA ATGGATCTCC AGAATCAGCA ACTGAAAGAG 
TTAAATGACT GGCTAACCAA AACAGAAGAG AGAACAAGGA AAATGGAGAA GGAGCCCCTT 
GGACCTGATA TTGAAGACCT AAAACGCCAA GTACAACAAC ATAAGGTGCT TCAAGAAGAC 
TTAGAACAGG AACAAGTCAG GGTCAATTCC CTCACTCATA TGGTGGTGGT AGTCGATGAA 
TCTAGTGGAG ACCATGCAAC TGCTGCTTTG GAAGAACAAC TTAAGGTACT GGGAGATCGA 
TGGGCAAACA TCTGTAGGTG GACAGAAGAT CGCTGGGTTC TTTTACAAGA CATCCTCCTA 
AAATGGCAGC GTTTTACTGA AGAACAGTGC CTTTTCAGTG CATGGCTTTC GGAGAAGGAA 
GATGCAGTGA ACAAGATTCA CACAACTGGC TTTAAGGATC AAAGTGAAGT GTTATCAAAT 
CTTCAGAAAC TGGCTGTCTT AAAAACAGAT CTGGAAAAGA AGAAGCAAAC CATGGACAAA 
CTCTGCTCAC TCAACCAAGA CCTTCTTTCA GCGCTGAAAA ACACAGTGGT AGCCCACAAG 
ATGGAAGCAT GGCTGGACAA CTTTGCCCAG CGCTGGGATA ATTTAGTCCA GAAACTTGAA 
AAAAGTTCAG CACAGATTTC ACAGGCTGTC ACCACCACTC AGCCATCACT AACACAGACA 
ACTGTAATGG AAACAGTAAC TATGGTGACC ACGAGGGAAC ACATCTTGGT AAAGCATGCC 
CAAGAGGAAC TGCCACCACC ACCCCCTCAG AAGAAGAGGC AGATTATCGT GGATTCTGAA 
ATTAGGAAAA GGTTGGATGT CGATATAACT GAACTTCACA GTTGGATTAC TCGTTCAGAA 
GCTGTGTTGC AGAGTCCTGA ATTTGCAATC TATCGGAAGG AAGGCAACTT CTCAGACCTT 
AAAGAAAAAG TCAATGCCAT AGAGCGAGAA AAAGCCGAGA AGTTCAGAAA ACTGCAAGAT 
GCCAGCAGAT CAGCTCAGGC CCTGGTGGAA CAGATGGTGA ATGAGGGTGT TAATGCTGAC 
AGCATCAAAC AAGCCTCCGA ACAACTGAAC AGCCGGTGGA TAGAGTTCTG CCAATTGCTA 
AGCGAGAGAC TTAACTGGCT GGAGTATCAG AACAACATCA TCACTTTCTA TAATCAGCTA 
CAACAATTGG AGCAGATGAC AACTACTGCT GAAAACTGGT TGAAAACCCA GCCTACCACC 
ACATCAGAGC CAACAGCAAT TAAAAGCCAG TTAAAAATTT GTAAGGATGA AATCAACCGA 
CTGTCAGCTC TTCAGCCTCA AATCGAGCGA TTAAAAATTC AAAGCATAGC CCTGAAAGAG 
AAAGGACAAG GGCCAATGTT CCTGGATGCA GACTTTGTGG CCTTTACAAA TCATTTTAAC 
CAAGTCTTTG CTGATGTGCA GGCAAGAGAA AAAGAGCTAC AAACAATTTT TGACAGTTTG 
CCACCCATGC GCTATCAGGA GACTATGAGT ACCATCCTGA CATGGATCCA GCAGTCAGAA 
ACCAAACTCT CTATACCTCA GGTTACTGTC ACTGAATATG ACATCATGGA ACAGAGACTC 
GGAGAGCTAC AGGCTTTACA AAGCTCTCTG CAAGAGCAAC AAAATGGCCT AAACTATCTC 
AGCACCACTG TGAAAGAGAT GTCAAAGAAA GCACCACTGT CTGATATTAG TCGGAAATAT 
CAATCAGAAT TTGAAGAGAT TGAGGGACGT TGGAAGAAGC TGTCTTCCCA GCTGGTTGAA 
CATTGTCAAA AGTTGGAGGA GCAAATGGCT AAACTTCGAA AAATTCAGAA TCACATAAAA 
ACTCTGAAGA AATGGATCAC TGAAGTCGAT GTTTTCCTGA AGGAGGAATG GCCTGCCCTT 
GGGGATTCAG AAATTCTGAA AAGACAGCTG AAACAGTGCA GGCTTTTAGT CAATGACATT 
CAGACCATCC AGCCTAGTCT CAACAGTGTC AATGAAGGGG CTCAGAAGAT GAAGAATGAA 
GCAGAACCAG AGTTTGCTGG CAGACTTGAG ACAGAGCTCC GAGAACTTAA CACCCAGTGG 
GATTACATGT GCCGCCAGGT CTATGCCAGG AAGGAAGCCT TAAAAGGAGG TTTGGATAAA 
ACTGTAAGTC TTCAGAAAGA TCTGTCAGAG ATGCATGAGT GGATGACACA AGCTGAAGAA 
GAATACCTAG AGAGAGATTT CGAATACAAG ACCCCTGATG AATTACAGAC AGCAGTTGAA 
GAGATGAAGA GAGCTAAAGA AGAGGCCCAG CAAAAAGAAG CAAAAGTGAA ACTCCTAACC 
GAGTCCGTCA ATAGTGTCAT AGCTCAGGCT CCACCTGCAG CACAAGAGGC CTTAAAAAAG 
GAACTTGACA CTCTCACCAC CAACTACCAG TGGCTCTGCA CCAGGCTCAA TGGCAAATGC 
AAGACCTTGG AAGAAGTTTG GGCGTGCTGG CATGAGTTAT TGTCCTACTT GGAGAAGGCA 
AACAAGTGGC TAAGTGAAGT AGAAGTCAAG CTTAAAACCA CTGAAAATAT TTCTGGGGGA 
GCTGAGGAAA TCGCCGAGGT GCTTGATTCG CTTGAAAATT TGATGCAACA TTCAGAGGAT 
AACCCGAATC AGATTCGCAT ATTGGCACAG ACCTTGACAG ATGGTGGAGT CATGGATGAA 
CTGATCAATG AGGAGCTTGA GACATTTAAT TCTCGTTGGA GAGAACTCCA TGAAGAGGCT 
GTGAGGAGGC AAAAGTTGCT TGAGCAGAGT ATCCAGTCGG CCCAGGAGAT AGAAAAATCC 
TTGCACTTAA TTCAGGAGTC CCTCTCTTCC ATTGACAAGC AGTTGGCAGC TTATATTGCT 
GACAAAGTGG ATGCAGCTCA GATGCCTCAG GAAGCCCAGA AAATCCAATC AGATTTGACA 
AGTCATGAGA TCAGTTTAGA AGAAATGAAG AAACATAACC AGGGAAAGGA GACTGCCCAA 
AGGGTACTAT CCCAAATTGA TGTGGCACAG AAAAAATTGC AGGATGTTTC CATGAAGTTT 
CGATTATTCC AGAAGCCAGC CAATTTTGAG CAGCGCCTAC AAGAAAGTAA AATGATTTTA 
GATGAAGTGA AGATGCATTT ACCTGCGTTG GAAACAAAGA GTGTGGAACA GGAAGTAGTA 
CAGTCACAGT TAAATCATTG TGTGAACTTG TATAAAAGTC TGAGTGAAGT GAAGTCTGAA 
GTGGAAATGG TAATAAAAAC TGGACGTCAG ATTGTACAGA AGAAGCAGAC GGAAAACCCG 
AAAGAGCTTG ATGAAAGAGT TACAGCTTTG AAATTGCATT ATAATGAGCT GGGAGCAAAG 
GTGACAGAAA GAAAGCAACA GTTGGAAAAA TGCTTGAAAT TGTCCCGTAA GATGCGAAAG 
GAAATGAATG CCCTGACAGA ATGGCTGGCA GCGACAGATA TGGAACTGAC GAAGAGATCG 
GCAGTTGAAG GAATGCCTAG TAATTTGGAT TCTGAAGTTG CCTGGGGAAA GGCTACTCAG 
AAAGAGATTG AGAAACAGAA GGTTCACCTA AAGAGTGTCA CAGAGGTAGG AGAGGCCTTG 
AAAACGGTTT TGGGCAAGAA GGAAATGTTG GTGGAAGATA AACTGAGTCT TCTGAATAGT 
AACTGGATAG CCGTCACTTC CCGAGCAGAA GAGTGGTTAA ACCTTTTATT GGAATACCAG 
AAACACATGG AAAACTTTGA CCAGAATGTG GATTACATCA CAAACTGGAT CATTCAGGCT 
GATGCACTTT TGGATGAATC TGAGAAAAAG AAACCTCAGC AAAAAGAAGA CATACTTAAG 
CGTTTAAAGG CTGAAATGAA TGACATACGT CCAAAGGTGG ATTCTACACG TGACCAAGCA 
GCAAACCTGA TGGCAAACCG CGGCGACCAC TGCAGGAAAG TAGTAGAGCC CAAAATCTCA 
GAGCTCAACC ATCGATTTGC AGCCATTTCT CACAGAATTA AGACTGGAAA GGCCTCCATT 
CCTTTGAAGG AATTGGAGCA GTTTAACTCA GATATACAAA AATTGCTTGA ACCACTGGAG 
GCTGAAATTC AGCAGGGGGT GAATCTGAAA GAGGAAGACT TCAATAAAGA TATGAGTGAA 
GACAATGAGG GTACTGTAAA AGAATTGTTG CAAAGAGGAG ACAACTTACA ACAAAGAATC 
ACAGATGAGA GAAAGCGAGA GGAAATAAAG ATAAAACAAC AGCTGTTACA GACAAAACAT 
AATGCTCTCA AGGATTTGAG GTCTCAAAGA AGAAAAAAGG CTCTAGAAAT TTCTCACCAG 
TGGTATCAGT ACAAGAGGCA GGCTGATGAT CTCCTGAAAT GCTTGGATGA CATTGAAAAA 
AAATTAGCCA GCCTACCTGA ACCCAGAGAT GAAAGGAAAA TAAAGGAAAT TGATCGTGAA 
TTGCAGAAGA AGAAAGAGGA GCTGAATGCA GTGCGTAGGC AAGCTGAGGG CTTGTCTGAG 
GATGGGGCCG CAATGGCAGT GGAGCCAACT CAGATCCAGC TCAGCAAGCG CTGGCGGGAA 
ATTGAGAGCA AATTTGCTCA GTTTCGAAGA CTCAACTTTG CACAAATTCA CACTGTCCAT 
GAAGAGTCAG TGGTGGCGAT GACTGAAGAC ATGCCTTTGG AAATTTCTTA TGTGCCTTCT 
ACTTACCTGA CTGAGATCAC TCATGTCTCA CAAGCCCTAT CAGAAGTGGA AGAGCTTCTT 
AATGCTCCCG ACCTTTGTGC TCAAGATTTT GAAGATCTCT TTAAACAAGA GGAATCCTTG 
AAGAACATAA AAGACAGCCT GCAACAAATC TCAGGTCGGA TTGACATCAT TCACAATAAA 
AAGACAGCAG CATTGCACAG TGCCACTCCT GCAGAAAGGG CAAAGCTCCA GGAAGCTCTC 
TCACGGCTTG ATTTCCAATG GGAAAGAGTT AACAATATGT ACAAGGACCG ACAAGGGAGA 
TTTGACAGAT CTGTGGAAAA ATGGCGGCGG TTTCATTATG ATATGAAGAT ACTTAATCAA 
TGGCTAACAG AAGCTGAACA GTTTCTCAAA AAGACACAAA TTCCTGAGAA TTGGGAACAT 
GCCAAATACA AATGGTATCT TAAGGAACTC CAGGATGGCA TTGGACAGCG GCAAAGTGTT 
GTCAGGGTAT TGAATGCAAC TGGGGAAGAA ATAATTCAAC AGTCCTCAAA AACAGATGCC 
AGTATTCTCC AAGAAAAACT GGGAAGCCTG AATCTGCGGT GGCAGGAGGT CTGCAAACAG 
CTGGCAGAAA GAAAAAAGAG GCTAGAGGAA CAGAAGAATA TCTTGTCAGA ATTTCAAAGA 
GATGTAAATG AATTTGTTTT ATGGTTGGAA GAAGCGGATA ACGTTGCTAA TATTCCACTT 
GAACCTGGAA ATGAGCAGCA GCTAAAAGAA AAACTTGAAC AAGTCAAGTT ACTGGCAGAA 
GAGTTGCCCC TGCGCCAGGG AATTCTAAAA CAATTAAATG AAACTGGAGG AACAGTGCTT 
GTAAGTGCTC CCCTAAGCCC AGAAGAGCAA GATAAACTTG AAAATAAGCT CAAGCAGACA 
AATCTTCAGT GGATAAAGGT TTCTAGAAAT CTGCCTGAGA AGCAAGAAGA AATTGAGGCA 
CACGTAAAAG ACCTTGGACA GCTGGAAGAG CAGTTAAATC ATCTGCTTCT ATGGCTGTCT 
CCTATTAGGA ATCAGTTGGA AATTTACAAT CAGCCAAATC AAACAGGACC ATTTGACATC 
AAGGAAATTG AAGTAGCAGT TCAAGCTAAA CAGCCGGATG TGGAAGGGAT TTTGTCTAAA 
GGGCAGCATT TGTACAAGGA AAAACCAGCC ACTCAGCCAG CGAAGAGAAA GCTGGAAGAT 
CTCAGCTCTG ATTGGAAGGT GGTAACTCAG TTGCTTCAAG AGCTGCGGGC AAAGCAGCCT 
GGCCCAGCTC CTGGACTGAC CACTGTCAGA GCCCCTCCCA GTCAGACTGT TACTCTGGTG 
ACACAACCCG CGGTTACCAA GGAAACTGCC ATCTCCAAAC TAGAAATGCC ATCTTCATTG 
CTGTTGGAGG TACCTGCACT GGCAGATTTC AACCGAGCTT GGACAGAACT TACCGACTGG 
CTGTCTCTGC TTGATCGAGT TATAAAATCA CAGAGGGTGA TGGTGGGTGA TCTTGAAGAC 
ATTAACGAGA TGATCATCAA GCAGAAGGCA ACGCTGCAGG ATTTGGAACA GAGGCGCCCC 
CAGTTGGAAG AACTCATTAC CGCTGCCCAG AATTTGAAAA ACAAGACCAG CAATCAAGAG 
GCTAGAACAA TCATTACTGA TCGAATTGAA AGAATTCAGA GTCAGTGGGA TGAAGTACAG 
GAACATCTTC AGAACCGGAG GCAACAGTTG AATGAAATGT TAAAGGATTC CACACAATGG 
CTGGAAGCTA AAGAGGAGGC TGAGCAGGTG TTGGGGCAGG CCAGAGCCAA GCTTGAGTCA 
TGGAAGGAGG CTCCCTACAC AGTAGATGCA ATCCAAAAGA AAATCACAGA AACCAAGCAG 
TTGGCCAAAG ACCTCCGCCA GTGGCAGATA AATGTAGATG TTGCAAATGA TTTGGCACTG 
AAACTTCTCC GAGATTATTC TGCAGATGAT ACCAGAAAAG TACACATGAT AACAGAGAAC 
ATCAATGCCT CTTGGGCAAG CATCCATAAA AGATTGAGTG AGCGAGAGGC TGCTCTGGAA 
GAAACCCACA GATTACTGCA ACAGTTCCCC TTGGACCTGG AGAAGTTCCT TGCCTGGCTT 
ACAGAAGCCG AAACAACTGC CAACGTCCTG CAGGATGCCA CCCATAAGGA AAGGCTTCTA 
GAAGATTCCA AGGGAGTAAG AGAGCTGATG AAACAATGGC AAGATCTCCA AGGAGAAATC 
GAAGCTCACA CAGATATCTA TCACAACCTG GACGAAAATG GCCAAAAAGT CCTGAGATCC 
CTGGAAGGTT CTGACGATGC AGCCTTGTTG CAAAGACGTT TGGATAACAT GAACTTCAAG 
TGGAGCGAAC TTCGGAAAAA GTCTCTCAAC ATTAGGTCTC ACTTGGAAGC CAGTTCTGAC 
CAGTGGAAGC GTCTGCACCT TTCTCTTCAG GAACTTCTGG TATGGCTCCA GCTGAAAGAT 
GATGAGTTAA GCCGGCAGGC ACCCATTGGA GGAGACTTTC CAGCGGTGCA GAAGCAGAAT 
GATGTACACA GGGCCTTCAA GAGGGAATTG AAAACGAAAG AACCTGTAAT CATGAGTACT 
CTTGAGACTG TACGAATATT TCTGACAGAG CAGCCTTTAG AAGGACTAGA GAAACTCTAC 
CAGGAGCCCA GAGAGCTGCC TCCTGAAGAG AGAGCCCAGA ATGTCACACG GCTCCTACGA 
AAGCAAGCTG AGGAGGTCAA CACTCAGTGG GAAAAACTGA ACGTGCACTC TGCAGACTGG 
CAGAGAAAAA TAGACGAGGC CCTCGAAAGA CTCCAGGAGC TTCAGGAAGC AACAGATGAG 
CTGGATCTCA AACTACGTCA GGCAGAGGTG ATCAAAGGAT CCTGGCAGCC TGTGGGTGAT 
CTCCTCATTG ACTCTCTCCA AGATCACCTC GAAAAAGTCA AGGCGCTTCG AGGAGAAATT 
ACACCTCTGA AAGAGAATGT CAGCTACGTC AATGACCTTG CTCGCCAACT CACTACGTTG 
GGCATTCAGC TGTCACCATA TAACCTCAAC ACTCTGGAAG ACCTGAACAC CAGATGGAAG 
CTTCTGCAGG TGGCCATTGA GGACCGCATC AGGCAGCTGC ATGAAGCGCA CAGGGACTTT 
GGACCAGCCT CCCAGCACTT CCTTTCCACT TCTGTCCAGG GTCCCTGGGA GAGAGCCATC 
TCACCAAACA AAGTGCCCTA CTATATCAAC CACGAGACCC AAACAACTTG CTGGGACCAT 
CCCAAAATGA CAGAGCTCTA CCAGTCTTTA GCTGACCTGA ATAATGTCAG GTTCTCAGCT 
TACAGGACTG CCATGAAACT CCGAAGACTG CAGAAGGCCC TTTGCTTGGA TCTCTTGAGC 
CTATCGGCTG CATGCGATGC CTTGGACCAG CACAACCTCA AGCAAAATGA CCAGCCCATG 
GATATCCTGC AGGTCATTAA CTGTCTGACC ACTATTTATG ATCGCCTAGA GCAAGAGCAC 
AACAATCTGG TCAACGTCCC TCTCTGCGTG GATATGTGTC TCAATTGGCT GCTGAATGTT 
TATGACACGG GACGAACGGG GAGGATCCGG GTCCTGTCTT TTAAAACTGG CATCATTTCT 
CTGTGTAAAG CCCATTTGGA AGACAAGTAC AGATACCTCT TCAAGCAAGT GGCAAGTTCG 
ACAGGATTTT GTGACCAGCG CAGGCTGGGC CTCCTCCTGC ATGACTCTAT CCAGATCCCA 
AGACAGTTGG GTGAAGTCGC ATCGTTCGGG GGCAGTAACA TTGAGCCGAG TGTCAGGAGC 
TGCTTCCAGT TTGCTAATAA TAAGCCTGAG ATCGAAGCGG CCCTCTTCCT AGACTGGATG 
CGCCTGGAGC CCCAGTCCAT GGTGTGGCTG CCTGTCCTGC ACCGAGTGGC TGCCGCGGAA 
ACTGCCAAGC ACCAGGCCAA GTGCAACATC TGCAAGGAGT GTCCCATCAT CGGATTCAGG 
TACAGGAGTC TAAAGCACTT TAATTATGAC ATCTGCCAAA GTTGCTTTTT TTCTGGTCGA 
GTTGCAAAAG GCCATAAAAT GCACTATCCC ATGGTGGAAT ACTGCACTCC GACTACATCG 
GGAGAAGATG TCCGTGACTT TGCCAAGGTA CTAAAAAACA AATTTCGAAC CAAAAGGTAT 
TTTGCGAAGC ATCCCCGAAT GGGCTACCTG CCAGTGCAGA CTGTCTTAGA GGGGGACAAC 
ATGGAAACAA ATCTGCAAGC AGAGTATGAT CGTCTAAAGC AGCAGCATGA ACACAAAGGC 
CTGTCCCCAC TGCCATCCCC TCCTGAAATG ATGCCTACTT CTCCCCAAAG TCCCCGGGAT 
GCTGAGCTCA TCGCTGAGGC CAAGCTGCTG CGTCAACACA AAGGCCGCCT GGAAGCCAGG 
ATGCAAATCC TTGAAGACCA TAACAAACAA CTGGAATCCC AGTTACACAG GCTCAGGCAG 
CTGCTGGAGC AACCCCAGGC AGAGGCCAAG GTGAATGGTA CAACGGTGTC TTCTCCTTCT 
ACCTCTCTTC AGAGGTCAGA TAGCAGTCAG CCTATGCTGC TCCGGGTGGT CGGCAGTCAG 
ACTTCAGAAT CCATGGGCGA GGAAGACCTG CTCAGCCCTC CCCAGGACAC AAGCACAGGG 
TTAGAGGAAG TGATGGAGCA GCTCAACCAC TCCTTCCCTA GTTCCAGAGG ACACAATGTA 
GGAAGCCTTT TCCACTTGGC AGATGATTTG GGCAGAGCGA TGGAGTCCTT AGTTTCAGTC 
ATGACAGATG AAGAAGGGGC AGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMD ( XM_014111342.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013966817.1 Canis lupus familiaris dystrophin (DMD), transcript variant X2, mRNA.

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CloneID	OCe45413
Clone ID Related Accession (Same CDS sequence)	XM_014111342.1 , XM_014111342.2
Accession Version	XM_014111342.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11094bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	dystrophin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTGGT GGGAAGAAGT AGAGGACTGT TATGAAAGAG AAGATGTTCA AAAGAAAACA 
TTCACAAAAT GGATAAATGC ACAGTTTTCT AAGTTTGGGA AGCAGCACAT AGAGAACCTC 
TTCAGTGACC TACAGGATGG GAGACGCCTC CTAGACCTTT TGGAAGGCCT GACAGGGCAA 
AAACTGCCAA AAGAAAAAGG ATCCACAAGA GTTCATGCCC TGAACAATGT CAACAAGGCA 
CTGCGCGTCT TGCAGAAAAA TAATGTTGAT TTAGTGAACA TTGGAAGTAC TGACATAGTA 
GATGGAAATC ACAAACTGAC TCTTGGTTTG ATTTGGAATA TAATCCTCCA CTGGCAGGTC 
AAAAATGTAA TGAAAAATAT CATGGCTGGA TTGCAACAAA CCAACAGTGA AAAGATTCTC 
CTGAGCTGGG TCCGACAATC AACTCGTAAT TATCCACAGG TTAATGTCAT TAACTTCACC 
ACCAGCTGGT CTGATGGCCT GGCTTTGAAC GCTCTCATCC ACAGTCATAG GCCAGACCTG 
TTTGATTGGA ATAGTGTGGT TTGCCAGCAG TCAGCCACAC AACGCCTGGA ACATGCATTC 
AACATTGCCA AATATCAATT AGGCATAGAG AAACTGCTTG ATCCTGAAGA TGTTGCCACC 
ACTTATCCAG ATAAGAAGTC CATCTTAATG TATATCACAT CACTCTTCCA AGTTTTGCCT 
CAACAAGTGA GCATTGAAGC CATCCAGGAA GTGGAAATGT TGCCAAGGCC ATCAAAAGTT 
ACTAGAGAAG AACATTTTCA ATTACATCAT CAAATGCACT ATTCTCAACA GATCACAGTC 
AGTCTAGCAC AGGGCTATGA ACGAGCCCCT TCCTCTCCTA AGCCTCGGTT CAAGAGCTAT 
GCCTACACAC AGGCTGCTTA TGTCACCACT TCTGACCCCA CACGGAGCCC ACTTCCTTCA 
CAGCATTTGG AAACTCCTGA AGACAAGTCA TTTGGCCGGT CATTGACAGA GACCGAAGCA 
AACCTGGACA GTTATCAAAC AGCTTTGGAA GAAGTACTCT CGTGGCTTCT TTCAGCTGAG 
GATGCACTGC AAGCCCAAGG AGAGATTTCT AATGATGTCG AAGAAGTGAA AGAACAATTT 
CATACTCATG AGGGATATAT GATGGACTTG ACATCCCATC AGGGACGGGT CGGTAATGTT 
CTCCAACTGG GAAGTCAACT GATTGGAACA GGGAAATTAT CAGAAGATGA AGAAACCGAA 
GTGCAGGAAC AAATGAATCT CCTCAATTCA AGATGGGAAT GCCTCAGGGT AGCTAGCATG 
GAAAAACAAA GCAATTTACA TAAAGTTCTA ATGGATCTCC AGAATCAGCA ACTGAAAGAG 
TTAAATGACT GGCTAACCAA AACAGAAGAG AGAACAAGGA AAATGGAGAA GGAGCCCCTT 
GGACCTGATA TTGAAGACCT AAAACGCCAA GTACAACAAC ATAAGGTGCT TCAAGAAGAC 
TTAGAACAGG AACAAGTCAG GGTCAATTCC CTCACTCATA TGGTGGTGGT AGTCGATGAA 
TCTAGTGGAG ACCATGCAAC TGCTGCTTTG GAAGAACAAC TTAAGGTACT GGGAGATCGA 
TGGGCAAACA TCTGTAGGTG GACAGAAGAT CGCTGGGTTC TTTTACAAGA CATCCTCCTA 
AAATGGCAGC GTTTTACTGA AGAACAGTGC CTTTTCAGTG CATGGCTTTC GGAGAAGGAA 
GATGCAGTGA ACAAGATTCA CACAACTGGC TTTAAGGATC AAAGTGAAGT GTTATCAAAT 
CTTCAGAAAC TGGCTGTCTT AAAAACAGAT CTGGAAAAGA AGAAGCAAAC CATGGACAAA 
CTCTGCTCAC TCAACCAAGA CCTTCTTTCA GCGCTGAAAA ACACAGTGGT AGCCCACAAG 
ATGGAAGCAT GGCTGGACAA CTTTGCCCAG CGCTGGGATA ATTTAGTCCA GAAACTTGAA 
AAAAGTTCAG CACAGATTTC ACAGGCTGTC ACCACCACTC AGCCATCACT AACACAGACA 
ACTGTAATGG AAACAGTAAC TATGGTGACC ACGAGGGAAC ACATCTTGGT AAAGCATGCC 
CAAGAGGAAC TGCCACCACC ACCCCCTCAG AAGAAGAGGC AGATTATCGT GGATTCTGAA 
ATTAGGAAAA GGTTGGATGT CGATATAACT GAACTTCACA GTTGGATTAC TCGTTCAGAA 
GCTGTGTTGC AGAGTCCTGA ATTTGCAATC TATCGGAAGG AAGGCAACTT CTCAGACCTT 
AAAGAAAAAG TCAATGCCAT AGAGCGAGAA AAAGCCGAGA AGTTCAGAAA ACTGCAAGAT 
GCCAGCAGAT CAGCTCAGGC CCTGGTGGAA CAGATGGTGA ATGAGGGTGT TAATGCTGAC 
AGCATCAAAC AAGCCTCCGA ACAACTGAAC AGCCGGTGGA TAGAGTTCTG CCAATTGCTA 
AGCGAGAGAC TTAACTGGCT GGAGTATCAG AACAACATCA TCACTTTCTA TAATCAGCTA 
CAACAATTGG AGCAGATGAC AACTACTGCT GAAAACTGGT TGAAAACCCA GCCTACCACC 
ACATCAGAGC CAACAGCAAT TAAAAGCCAG TTAAAAATTT GTAAGGATGA AATCAACCGA 
CTGTCAGCTC TTCAGCCTCA AATCGAGCGA TTAAAAATTC AAAGCATAGC CCTGAAAGAG 
AAAGGACAAG GGCCAATGTT CCTGGATGCA GACTTTGTGG CCTTTACAAA TCATTTTAAC 
CAAGTCTTTG CTGATGTGCA GGCAAGAGAA AAAGAGCTAC AAACAATTTT TGACAGTTTG 
CCACCCATGC GCTATCAGGA GACTATGAGT ACCATCCTGA CATGGATCCA GCAGTCAGAA 
ACCAAACTCT CTATACCTCA GGTTACTGTC ACTGAATATG ACATCATGGA ACAGAGACTC 
GGAGAGCTAC AGGCTTTACA AAGCTCTCTG CAAGAGCAAC AAAATGGCCT AAACTATCTC 
AGCACCACTG TGAAAGAGAT GTCAAAGAAA GCACCACTGT CTGATATTAG TCGGAAATAT 
CAATCAGAAT TTGAAGAGAT TGAGGGACGT TGGAAGAAGC TGTCTTCCCA GCTGGTTGAA 
CATTGTCAAA AGTTGGAGGA GCAAATGGCT AAACTTCGAA AAATTCAGAA TCACATAAAA 
ACTCTGAAGA AATGGATCAC TGAAGTCGAT GTTTTCCTGA AGGAGGAATG GCCTGCCCTT 
GGGGATTCAG AAATTCTGAA AAGACAGCTG AAACAGTGCA GGCTTTTAGT CAATGACATT 
CAGACCATCC AGCCTAGTCT CAACAGTGTC AATGAAGGGG CTCAGAAGAT GAAGAATGAA 
GCAGAACCAG AGTTTGCTGG CAGACTTGAG ACAGAGCTCC GAGAACTTAA CACCCAGTGG 
GATTACATGT GCCGCCAGGT CTATGCCAGG AAGGAAGCCT TAAAAGGAGG TTTGGATAAA 
ACTGTAAGTC TTCAGAAAGA TCTGTCAGAG ATGCATGAGT GGATGACACA AGCTGAAGAA 
GAATACCTAG AGAGAGATTT CGAATACAAG ACCCCTGATG AATTACAGAC AGCAGTTGAA 
GAGATGAAGA GAGCTAAAGA AGAGGCCCAG CAAAAAGAAG CAAAAGTGAA ACTCCTAACC 
GAGTCCGTCA ATAGTGTCAT AGCTCAGGCT CCACCTGCAG CACAAGAGGC CTTAAAAAAG 
GAACTTGACA CTCTCACCAC CAACTACCAG TGGCTCTGCA CCAGGCTCAA TGGCAAATGC 
AAGACCTTGG AAGAAGTTTG GGCGTGCTGG CATGAGTTAT TGTCCTACTT GGAGAAGGCA 
AACAAGTGGC TAAGTGAAGT AGAAGTCAAG CTTAAAACCA CTGAAAATAT TTCTGGGGGA 
GCTGAGGAAA TCGCCGAGGT GCTTGATTCG CTTGAAAATT TGATGCAACA TTCAGAGGAT 
AACCCGAATC AGATTCGCAT ATTGGCACAG ACCTTGACAG ATGGTGGAGT CATGGATGAA 
CTGATCAATG AGGAGCTTGA GACATTTAAT TCTCGTTGGA GAGAACTCCA TGAAGAGGCT 
GTGAGGAGGC AAAAGTTGCT TGAGCAGAGT ATCCAGTCGG CCCAGGAGAT AGAAAAATCC 
TTGCACTTAA TTCAGGAGTC CCTCTCTTCC ATTGACAAGC AGTTGGCAGC TTATATTGCT 
GACAAAGTGG ATGCAGCTCA GATGCCTCAG GAAGCCCAGA AAATCCAATC AGATTTGACA 
AGTCATGAGA TCAGTTTAGA AGAAATGAAG AAACATAACC AGGGAAAGGA GACTGCCCAA 
AGGGTACTAT CCCAAATTGA TGTGGCACAG AAAAAATTGC AGGATGTTTC CATGAAGTTT 
CGATTATTCC AGAAGCCAGC CAATTTTGAG CAGCGCCTAC AAGAAAGTAA AATGATTTTA 
GATGAAGTGA AGATGCATTT ACCTGCGTTG GAAACAAAGA GTGTGGAACA GGAAGTAGTA 
CAGTCACAGT TAAATCATTG TGTGAACTTG TATAAAAGTC TGAGTGAAGT GAAGTCTGAA 
GTGGAAATGG TAATAAAAAC TGGACGTCAG ATTGTACAGA AGAAGCAGAC GGAAAACCCG 
AAAGAGCTTG ATGAAAGAGT TACAGCTTTG AAATTGCATT ATAATGAGCT GGGAGCAAAG 
GTGACAGAAA GAAAGCAACA GTTGGAAAAA TGCTTGAAAT TGTCCCGTAA GATGCGAAAG 
GAAATGAATG CCCTGACAGA ATGGCTGGCA GCGACAGATA TGGAACTGAC GAAGAGATCG 
GCAGTTGAAG GAATGCCTAG TAATTTGGAT TCTGAAGTTG CCTGGGGAAA GGCTACTCAG 
AAAGAGATTG AGAAACAGAA GGTTCACCTA AAGAGTGTCA CAGAGGTAGG AGAGGCCTTG 
AAAACGGTTT TGGGCAAGAA GGAAATGTTG GTGGAAGATA AACTGAGTCT TCTGAATAGT 
AACTGGATAG CCGTCACTTC CCGAGCAGAA GAGTGGTTAA ACCTTTTATT GGAATACCAG 
AAACACATGG AAAACTTTGA CCAGAATGTG GATTACATCA CAAACTGGAT CATTCAGGCT 
GATGCACTTT TGGATGAATC TGAGAAAAAG AAACCTCAGC AAAAAGAAGA CATACTTAAG 
CGTTTAAAGG CTGAAATGAA TGACATACGT CCAAAGGTGG ATTCTACACG TGACCAAGCA 
GCAAACCTGA TGGCAAACCG CGGCGACCAC TGCAGGAAAG TAGTAGAGCC CAAAATCTCA 
GAGCTCAACC ATCGATTTGC AGCCATTTCT CACAGAATTA AGACTGGAAA GGCCTCCATT 
CCTTTGAAGG AATTGGAGCA GTTTAACTCA GATATACAAA AATTGCTTGA ACCACTGGAG 
GCTGAAATTC AGCAGGGGGT GAATCTGAAA GAGGAAGACT TCAATAAAGA TATGAGTGAA 
GACAATGAGG GTACTGTAAA AGAATTGTTG CAAAGAGGAG ACAACTTACA ACAAAGAATC 
ACAGATGAGA GAAAGCGAGA GGAAATAAAG ATAAAACAAC AGCTGTTACA GACAAAACAT 
AATGCTCTCA AGGATTTGAG GTCTCAAAGA AGAAAAAAGG CTCTAGAAAT TTCTCACCAG 
TGGTATCAGT ACAAGAGGCA GGCTGATGAT CTCCTGAAAT GCTTGGATGA CATTGAAAAA 
AAATTAGCCA GCCTACCTGA ACCCAGAGAT GAAAGGAAAA TAAAGGAAAT TGATCGTGAA 
TTGCAGAAGA AGAAAGAGGA GCTGAATGCA GTGCGTAGGC AAGCTGAGGG CTTGTCTGAG 
GATGGGGCCG CAATGGCAGT GGAGCCAACT CAGATCCAGC TCAGCAAGCG CTGGCGGGAA 
ATTGAGAGCA AATTTGCTCA GTTTCGAAGA CTCAACTTTG CACAAATTCA CACTGTCCAT 
GAAGAGTCAG TGGTGGCGAT GACTGAAGAC ATGCCTTTGG AAATTTCTTA TGTGCCTTCT 
ACTTACCTGA CTGAGATCAC TCATGTCTCA CAAGCCCTAT CAGAAGTGGA AGAGCTTCTT 
AATGCTCCCG ACCTTTGTGC TCAAGATTTT GAAGATCTCT TTAAACAAGA GGAATCCTTG 
AAGAACATAA AAGACAGCCT GCAACAAATC TCAGGTCGGA TTGACATCAT TCACAATAAA 
AAGACAGCAG CATTGCACAG TGCCACTCCT GCAGAAAGGG CAAAGCTCCA GGAAGCTCTC 
TCACGGCTTG ATTTCCAATG GGAAAGAGTT AACAATATGT ACAAGGACCG ACAAGGGAGA 
TTTGACAGAT CTGTGGAAAA ATGGCGGCGG TTTCATTATG ATATGAAGAT ACTTAATCAA 
TGGCTAACAG AAGCTGAACA GTTTCTCAAA AAGACACAAA TTCCTGAGAA TTGGGAACAT 
GCCAAATACA AATGGTATCT TAAGGAACTC CAGGATGGCA TTGGACAGCG GCAAAGTGTT 
GTCAGGGTAT TGAATGCAAC TGGGGAAGAA ATAATTCAAC AGTCCTCAAA AACAGATGCC 
AGTATTCTCC AAGAAAAACT GGGAAGCCTG AATCTGCGGT GGCAGGAGGT CTGCAAACAG 
CTGGCAGAAA GAAAAAAGAG GCTAGAGGAA CAGAAGAATA TCTTGTCAGA ATTTCAAAGA 
GATGTAAATG AATTTGTTTT ATGGTTGGAA GAAGCGGATA ACGTTGCTAA TATTCCACTT 
GAACCTGGAA ATGAGCAGCA GCTAAAAGAA AAACTTGAAC AAGTCAAGTT ACTGGCAGAA 
GAGTTGCCCC TGCGCCAGGG AATTCTAAAA CAATTAAATG AAACTGGAGG AACAGTGCTT 
GTAAGTGCTC CCCTAAGCCC AGAAGAGCAA GATAAACTTG AAAATAAGCT CAAGCAGACA 
AATCTTCAGT GGATAAAGGT TTCTAGAAAT CTGCCTGAGA AGCAAGAAGA AATTGAGGCA 
CACGTAAAAG ACCTTGGACA GCTGGAAGAG CAGTTAAATC ATCTGCTTCT ATGGCTGTCT 
CCTATTAGGA ATCAGTTGGA AATTTACAAT CAGCCAAATC AAACAGGACC ATTTGACATC 
AAGGAAATTG AAGTAGCAGT TCAAGCTAAA CAGCCGGATG TGGAAGGGAT TTTGTCTAAA 
GGGCAGCATT TGTACAAGGA AAAACCAGCC ACTCAGCCAG CGAAGAGAAA GCTGGAAGAT 
CTCAGCTCTG ATTGGAAGGT GGTAACTCAG TTGCTTCAAG AGCTGCGGGC AAAGCAGCCT 
GGCCCAGCTC CTGGACTGAC CACTGTCAGA GCCCCTCCCA GTCAGACTGT TACTCTGGTG 
ACACAACCCG CGGTTACCAA GGAAACTGCC ATCTCCAAAC TAGAAATGCC ATCTTCATTG 
CTGTTGGAGG TACCTGCACT GGCAGATTTC AACCGAGCTT GGACAGAACT TACCGACTGG 
CTGTCTCTGC TTGATCGAGT TATAAAATCA CAGAGGGTGA TGGTGGGTGA TCTTGAAGAC 
ATTAACGAGA TGATCATCAA GCAGAAGGCA ACGCTGCAGG ATTTGGAACA GAGGCGCCCC 
CAGTTGGAAG AACTCATTAC CGCTGCCCAG AATTTGAAAA ACAAGACCAG CAATCAAGAG 
GCTAGAACAA TCATTACTGA TCGAATTGAA AGAATTCAGA GTCAGTGGGA TGAAGTACAG 
GAACATCTTC AGAACCGGAG GCAACAGTTG AATGAAATGT TAAAGGATTC CACACAATGG 
CTGGAAGCTA AAGAGGAGGC TGAGCAGGTG TTGGGGCAGG CCAGAGCCAA GCTTGAGTCA 
TGGAAGGAGG CTCCCTACAC AGTAGATGCA ATCCAAAAGA AAATCACAGA AACCAAGTTG 
GCCAAAGACC TCCGCCAGTG GCAGATAAAT GTAGATGTTG CAAATGATTT GGCACTGAAA 
CTTCTCCGAG ATTATTCTGC AGATGATACC AGAAAAGTAC ACATGATAAC AGAGAACATC 
AATGCCTCTT GGGCAAGCAT CCATAAAAGA TTGAGTGAGC GAGAGGCTGC TCTGGAAGAA 
ACCCACAGAT TACTGCAACA GTTCCCCTTG GACCTGGAGA AGTTCCTTGC CTGGCTTACA 
GAAGCCGAAA CAACTGCCAA CGTCCTGCAG GATGCCACCC ATAAGGAAAG GCTTCTAGAA 
GATTCCAAGG GAGTAAGAGA GCTGATGAAA CAATGGCAAG ATCTCCAAGG AGAAATCGAA 
GCTCACACAG ATATCTATCA CAACCTGGAC GAAAATGGCC AAAAAGTCCT GAGATCCCTG 
GAAGGTTCTG ACGATGCAGC CTTGTTGCAA AGACGTTTGG ATAACATGAA CTTCAAGTGG 
AGCGAACTTC GGAAAAAGTC TCTCAACATT AGGTCTCACT TGGAAGCCAG TTCTGACCAG 
TGGAAGCGTC TGCACCTTTC TCTTCAGGAA CTTCTGGTAT GGCTCCAGCT GAAAGATGAT 
GAGTTAAGCC GGCAGGCACC CATTGGAGGA GACTTTCCAG CGGTGCAGAA GCAGAATGAT 
GTACACAGGG CCTTCAAGAG GGAATTGAAA ACGAAAGAAC CTGTAATCAT GAGTACTCTT 
GAGACTGTAC GAATATTTCT GACAGAGCAG CCTTTAGAAG GACTAGAGAA ACTCTACCAG 
GAGCCCAGAG AGCTGCCTCC TGAAGAGAGA GCCCAGAATG TCACACGGCT CCTACGAAAG 
CAAGCTGAGG AGGTCAACAC TCAGTGGGAA AAACTGAACG TGCACTCTGC AGACTGGCAG 
AGAAAAATAG ACGAGGCCCT CGAAAGACTC CAGGAGCTTC AGGAAGCAAC AGATGAGCTG 
GATCTCAAAC TACGTCAGGC AGAGGTGATC AAAGGATCCT GGCAGCCTGT GGGTGATCTC 
CTCATTGACT CTCTCCAAGA TCACCTCGAA AAAGTCAAGG CGCTTCGAGG AGAAATTACA 
CCTCTGAAAG AGAATGTCAG CTACGTCAAT GACCTTGCTC GCCAACTCAC TACGTTGGGC 
ATTCAGCTGT CACCATATAA CCTCAACACT CTGGAAGACC TGAACACCAG ATGGAAGCTT 
CTGCAGGTGG CCATTGAGGA CCGCATCAGG CAGCTGCATG AAGCGCACAG GGACTTTGGA 
CCAGCCTCCC AGCACTTCCT TTCCACTTCT GTCCAGGGTC CCTGGGAGAG AGCCATCTCA 
CCAAACAAAG TGCCCTACTA TATCAACCAC GAGACCCAAA CAACTTGCTG GGACCATCCC 
AAAATGACAG AGCTCTACCA GTCTTTAGCT GACCTGAATA ATGTCAGGTT CTCAGCTTAC 
AGGACTGCCA TGAAACTCCG AAGACTGCAG AAGGCCCTTT GCTTGGATCT CTTGAGCCTA 
TCGGCTGCAT GCGATGCCTT GGACCAGCAC AACCTCAAGC AAAATGACCA GCCCATGGAT 
ATCCTGCAGG TCATTAACTG TCTGACCACT ATTTATGATC GCCTAGAGCA AGAGCACAAC 
AATCTGGTCA ACGTCCCTCT CTGCGTGGAT ATGTGTCTCA ATTGGCTGCT GAATGTTTAT 
GACACGGGAC GAACGGGGAG GATCCGGGTC CTGTCTTTTA AAACTGGCAT CATTTCTCTG 
TGTAAAGCCC ATTTGGAAGA CAAGTACAGA TACCTCTTCA AGCAAGTGGC AAGTTCGACA 
GGATTTTGTG ACCAGCGCAG GCTGGGCCTC CTCCTGCATG ACTCTATCCA GATCCCAAGA 
CAGTTGGGTG AAGTCGCATC GTTCGGGGGC AGTAACATTG AGCCGAGTGT CAGGAGCTGC 
TTCCAGTTTG CTAATAATAA GCCTGAGATC GAAGCGGCCC TCTTCCTAGA CTGGATGCGC 
CTGGAGCCCC AGTCCATGGT GTGGCTGCCT GTCCTGCACC GAGTGGCTGC CGCGGAAACT 
GCCAAGCACC AGGCCAAGTG CAACATCTGC AAGGAGTGTC CCATCATCGG ATTCAGGTAC 
AGGAGTCTAA AGCACTTTAA TTATGACATC TGCCAAAGTT GCTTTTTTTC TGGTCGAGTT 
GCAAAAGGCC ATAAAATGCA CTATCCCATG GTGGAATACT GCACTCCGAC TACATCGGGA 
GAAGATGTCC GTGACTTTGC CAAGGTACTA AAAAACAAAT TTCGAACCAA AAGGTATTTT 
GCGAAGCATC CCCGAATGGG CTACCTGCCA GTGCAGACTG TCTTAGAGGG GGACAACATG 
GAAACTCCTG TCACTCTGAT CAACTTCTGG CCGGTAGATT CTGCGCCTGC CTCGTCCCCT 
CAGCTTTCAC ACGATGATAC TCATTCACGC ATTGAGCATT ATGCTAGCAG GCTAGCAGAA 
ATGGAAAACA GCAATGGATC TTATCTAAAT GATAGCATCT CTCCTAATGA GAGCATAGAT 
GATGAACATT TGTTAATCCA GCATTACTGC CAAAGTTTGA ACCAGGACTC CCCCCTGAGC 
CAGCCTCGTA GTCCTGCCCA GATCTTGATT TCCTTAGAGA GTGAGGAAAG AGGGGAGCTA 
GAGAGAATCC TAGCAGATCT TGAGGAAGAA AACAGAAATC TGCAAGCAGA GTATGATCGT 
CTAAAGCAGC AGCATGAACA CAAAGGCCTG TCCCCACTGC CATCCCCTCC TGAAATGATG 
CCTACTTCTC CCCAAAGTCC CCGGGATGCT GAGCTCATCG CTGAGGCCAA GCTGCTGCGT 
CAACACAAAG GCCGCCTGGA AGCCAGGATG CAAATCCTTG AAGACCATAA CAAACAACTG 
GAATCCCAGT TACACAGGCT CAGGCAGCTG CTGGAGCAAC CCCAGGCAGA GGCCAAGGTG 
AATGGTACAA CGGTGTCTTC TCCTTCTACC TCTCTTCAGA GGTCAGATAG CAGTCAGCCT 
ATGCTGCTCC GGGTGGTCGG CAGTCAGACT TCAGAATCCA TGGGCGAGGA AGACCTGCTC 
AGCCCTCCCC AGGACACAAG CACAGGGTTA GAGGAAGTGA TGGAGCAGCT CAACCACTCC 
TTCCCTAGTT CCAGAGGACA CAATGTAGGA AGCCTTTTCC ACTTGGCAGA TGATTTGGGC 
AGAGCGATGG AGTCCTTAGT TTCAGTCATG ACAGATGAAG AAGGGGCAGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013966817.1
CDS	248..11341
Translation	MLWWEEVEDCYEREDVQKKTFTKWINAQFSKFGKQHIENLFSDLQDGRRLLDLLEGLTGQKLPKEKGSTRVHALNNVNKALRVLQKNNVDLVNIGSTDIVDGNHKLTLGLIWNIILHWQVKNVMKNIMAGLQQTNSEKILLSWVRQSTRNYPQVNVINFTTSWSDGLALNALIHSHRPDLFDWNSVVCQQSATQRLEHAFNIAKYQLGIEKLLDPEDVATTYPDKKSILMYITSLFQVLPQQVSIEAIQEVEMLPRPSKVTREEHFQLHHQMHYSQQITVSLAQGYERAPSSPKPRFKSYAYTQAAYVTTSDPTRSPLPSQHLETPEDKSFGRSLTETEANLDSYQTALEEVLSWLLSAEDALQAQGEISNDVEEVKEQFHTHEGYMMDLTSHQGRVGNVLQLGSQLIGTGKLSEDEETEVQEQMNLLNSRWECLRVASMEKQSNLHKVLMDLQNQQLKELNDWLTKTEERTRKMEKEPLGPDIEDLKRQVQQHKVLQEDLEQEQVRVNSLTHMVVVVDESSGDHATAALEEQLKVLGDRWANICRWTEDRWVLLQDILLKWQRFTEEQCLFSAWLSEKEDAVNKIHTTGFKDQSEVLSNLQKLAVLKTDLEKKKQTMDKLCSLNQDLLSALKNTVVAHKMEAWLDNFAQRWDNLVQKLEKSSAQISQAVTTTQPSLTQTTVMETVTMVTTREHILVKHAQEELPPPPPQKKRQIIVDSEIRKRLDVDITELHSWITRSEAVLQSPEFAIYRKEGNFSDLKEKVNAIEREKAEKFRKLQDASRSAQALVEQMVNEGVNADSIKQASEQLNSRWIEFCQLLSERLNWLEYQNNIITFYNQLQQLEQMTTTAENWLKTQPTTTSEPTAIKSQLKICKDEINRLSALQPQIERLKIQSIALKEKGQGPMFLDADFVAFTNHFNQVFADVQAREKELQTIFDSLPPMRYQETMSTILTWIQQSETKLSIPQVTVTEYDIMEQRLGELQALQSSLQEQQNGLNYLSTTVKEMSKKAPLSDISRKYQSEFEEIEGRWKKLSSQLVEHCQKLEEQMAKLRKIQNHIKTLKKWITEVDVFLKEEWPALGDSEILKRQLKQCRLLVNDIQTIQPSLNSVNEGAQKMKNEAEPEFAGRLETELRELNTQWDYMCRQVYARKEALKGGLDKTVSLQKDLSEMHEWMTQAEEEYLERDFEYKTPDELQTAVEEMKRAKEEAQQKEAKVKLLTESVNSVIAQAPPAAQEALKKELDTLTTNYQWLCTRLNGKCKTLEEVWACWHELLSYLEKANKWLSEVEVKLKTTENISGGAEEIAEVLDSLENLMQHSEDNPNQIRILAQTLTDGGVMDELINEELETFNSRWRELHEEAVRRQKLLEQSIQSAQEIEKSLHLIQESLSSIDKQLAAYIADKVDAAQMPQEAQKIQSDLTSHEISLEEMKKHNQGKETAQRVLSQIDVAQKKLQDVSMKFRLFQKPANFEQRLQESKMILDEVKMHLPALETKSVEQEVVQSQLNHCVNLYKSLSEVKSEVEMVIKTGRQIVQKKQTENPKELDERVTALKLHYNELGAKVTERKQQLEKCLKLSRKMRKEMNALTEWLAATDMELTKRSAVEGMPSNLDSEVAWGKATQKEIEKQKVHLKSVTEVGEALKTVLGKKEMLVEDKLSLLNSNWIAVTSRAEEWLNLLLEYQKHMENFDQNVDYITNWIIQADALLDESEKKKPQQKEDILKRLKAEMNDIRPKVDSTRDQAANLMANRGDHCRKVVEPKISELNHRFAAISHRIKTGKASIPLKELEQFNSDIQKLLEPLEAEIQQGVNLKEEDFNKDMSEDNEGTVKELLQRGDNLQQRITDERKREEIKIKQQLLQTKHNALKDLRSQRRKKALEISHQWYQYKRQADDLLKCLDDIEKKLASLPEPRDERKIKEIDRELQKKKEELNAVRRQAEGLSEDGAAMAVEPTQIQLSKRWREIESKFAQFRRLNFAQIHTVHEESVVAMTEDMPLEISYVPSTYLTEITHVSQALSEVEELLNAPDLCAQDFEDLFKQEESLKNIKDSLQQISGRIDIIHNKKTAALHSATPAERAKLQEALSRLDFQWERVNNMYKDRQGRFDRSVEKWRRFHYDMKILNQWLTEAEQFLKKTQIPENWEHAKYKWYLKELQDGIGQRQSVVRVLNATGEEIIQQSSKTDASILQEKLGSLNLRWQEVCKQLAERKKRLEEQKNILSEFQRDVNEFVLWLEEADNVANIPLEPGNEQQLKEKLEQVKLLAEELPLRQGILKQLNETGGTVLVSAPLSPEEQDKLENKLKQTNLQWIKVSRNLPEKQEEIEAHVKDLGQLEEQLNHLLLWLSPIRNQLEIYNQPNQTGPFDIKEIEVAVQAKQPDVEGILSKGQHLYKEKPATQPAKRKLEDLSSDWKVVTQLLQELRAKQPGPAPGLTTVRAPPSQTVTLVTQPAVTKETAISKLEMPSSLLLEVPALADFNRAWTELTDWLSLLDRVIKSQRVMVGDLEDINEMIIKQKATLQDLEQRRPQLEELITAAQNLKNKTSNQEARTIITDRIERIQSQWDEVQEHLQNRRQQLNEMLKDSTQWLEAKEEAEQVLGQARAKLESWKEAPYTVDAIQKKITETKLAKDLRQWQINVDVANDLALKLLRDYSADDTRKVHMITENINASWASIHKRLSEREAALEETHRLLQQFPLDLEKFLAWLTEAETTANVLQDATHKERLLEDSKGVRELMKQWQDLQGEIEAHTDIYHNLDENGQKVLRSLEGSDDAALLQRRLDNMNFKWSELRKKSLNIRSHLEASSDQWKRLHLSLQELLVWLQLKDDELSRQAPIGGDFPAVQKQNDVHRAFKRELKTKEPVIMSTLETVRIFLTEQPLEGLEKLYQEPRELPPEERAQNVTRLLRKQAEEVNTQWEKLNVHSADWQRKIDEALERLQELQEATDELDLKLRQAEVIKGSWQPVGDLLIDSLQDHLEKVKALRGEITPLKENVSYVNDLARQLTTLGIQLSPYNLNTLEDLNTRWKLLQVAIEDRIRQLHEAHRDFGPASQHFLSTSVQGPWERAISPNKVPYYINHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQVINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPVTLINFWPVDSAPASSPQLSHDDTHSRIEHYASRLAEMENSNGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKQQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDTSTGLEEVMEQLNHSFPSSRGHNVGSLFHLADDLGRAMESLVSVMTDEEGAE

Target ORF information:

RefSeq Version	XM_014111342.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris dystrophin (DMD), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111342.1

ORF Insert Sequence:

ATGCTTTGGT GGGAAGAAGT AGAGGACTGT TATGAAAGAG AAGATGTTCA AAAGAAAACA 
TTCACAAAAT GGATAAATGC ACAGTTTTCT AAGTTTGGGA AGCAGCACAT AGAGAACCTC 
TTCAGTGACC TACAGGATGG GAGACGCCTC CTAGACCTTT TGGAAGGCCT GACAGGGCAA 
AAACTGCCAA AAGAAAAAGG ATCCACAAGA GTTCATGCCC TGAACAATGT CAACAAGGCA 
CTGCGCGTCT TGCAGAAAAA TAATGTTGAT TTAGTGAACA TTGGAAGTAC TGACATAGTA 
GATGGAAATC ACAAACTGAC TCTTGGTTTG ATTTGGAATA TAATCCTCCA CTGGCAGGTC 
AAAAATGTAA TGAAAAATAT CATGGCTGGA TTGCAACAAA CCAACAGTGA AAAGATTCTC 
CTGAGCTGGG TCCGACAATC AACTCGTAAT TATCCACAGG TTAATGTCAT TAACTTCACC 
ACCAGCTGGT CTGATGGCCT GGCTTTGAAC GCTCTCATCC ACAGTCATAG GCCAGACCTG 
TTTGATTGGA ATAGTGTGGT TTGCCAGCAG TCAGCCACAC AACGCCTGGA ACATGCATTC 
AACATTGCCA AATATCAATT AGGCATAGAG AAACTGCTTG ATCCTGAAGA TGTTGCCACC 
ACTTATCCAG ATAAGAAGTC CATCTTAATG TATATCACAT CACTCTTCCA AGTTTTGCCT 
CAACAAGTGA GCATTGAAGC CATCCAGGAA GTGGAAATGT TGCCAAGGCC ATCAAAAGTT 
ACTAGAGAAG AACATTTTCA ATTACATCAT CAAATGCACT ATTCTCAACA GATCACAGTC 
AGTCTAGCAC AGGGCTATGA ACGAGCCCCT TCCTCTCCTA AGCCTCGGTT CAAGAGCTAT 
GCCTACACAC AGGCTGCTTA TGTCACCACT TCTGACCCCA CACGGAGCCC ACTTCCTTCA 
CAGCATTTGG AAACTCCTGA AGACAAGTCA TTTGGCCGGT CATTGACAGA GACCGAAGCA 
AACCTGGACA GTTATCAAAC AGCTTTGGAA GAAGTACTCT CGTGGCTTCT TTCAGCTGAG 
GATGCACTGC AAGCCCAAGG AGAGATTTCT AATGATGTCG AAGAAGTGAA AGAACAATTT 
CATACTCATG AGGGATATAT GATGGACTTG ACATCCCATC AGGGACGGGT CGGTAATGTT 
CTCCAACTGG GAAGTCAACT GATTGGAACA GGGAAATTAT CAGAAGATGA AGAAACCGAA 
GTGCAGGAAC AAATGAATCT CCTCAATTCA AGATGGGAAT GCCTCAGGGT AGCTAGCATG 
GAAAAACAAA GCAATTTACA TAAAGTTCTA ATGGATCTCC AGAATCAGCA ACTGAAAGAG 
TTAAATGACT GGCTAACCAA AACAGAAGAG AGAACAAGGA AAATGGAGAA GGAGCCCCTT 
GGACCTGATA TTGAAGACCT AAAACGCCAA GTACAACAAC ATAAGGTGCT TCAAGAAGAC 
TTAGAACAGG AACAAGTCAG GGTCAATTCC CTCACTCATA TGGTGGTGGT AGTCGATGAA 
TCTAGTGGAG ACCATGCAAC TGCTGCTTTG GAAGAACAAC TTAAGGTACT GGGAGATCGA 
TGGGCAAACA TCTGTAGGTG GACAGAAGAT CGCTGGGTTC TTTTACAAGA CATCCTCCTA 
AAATGGCAGC GTTTTACTGA AGAACAGTGC CTTTTCAGTG CATGGCTTTC GGAGAAGGAA 
GATGCAGTGA ACAAGATTCA CACAACTGGC TTTAAGGATC AAAGTGAAGT GTTATCAAAT 
CTTCAGAAAC TGGCTGTCTT AAAAACAGAT CTGGAAAAGA AGAAGCAAAC CATGGACAAA 
CTCTGCTCAC TCAACCAAGA CCTTCTTTCA GCGCTGAAAA ACACAGTGGT AGCCCACAAG 
ATGGAAGCAT GGCTGGACAA CTTTGCCCAG CGCTGGGATA ATTTAGTCCA GAAACTTGAA 
AAAAGTTCAG CACAGATTTC ACAGGCTGTC ACCACCACTC AGCCATCACT AACACAGACA 
ACTGTAATGG AAACAGTAAC TATGGTGACC ACGAGGGAAC ACATCTTGGT AAAGCATGCC 
CAAGAGGAAC TGCCACCACC ACCCCCTCAG AAGAAGAGGC AGATTATCGT GGATTCTGAA 
ATTAGGAAAA GGTTGGATGT CGATATAACT GAACTTCACA GTTGGATTAC TCGTTCAGAA 
GCTGTGTTGC AGAGTCCTGA ATTTGCAATC TATCGGAAGG AAGGCAACTT CTCAGACCTT 
AAAGAAAAAG TCAATGCCAT AGAGCGAGAA AAAGCCGAGA AGTTCAGAAA ACTGCAAGAT 
GCCAGCAGAT CAGCTCAGGC CCTGGTGGAA CAGATGGTGA ATGAGGGTGT TAATGCTGAC 
AGCATCAAAC AAGCCTCCGA ACAACTGAAC AGCCGGTGGA TAGAGTTCTG CCAATTGCTA 
AGCGAGAGAC TTAACTGGCT GGAGTATCAG AACAACATCA TCACTTTCTA TAATCAGCTA 
CAACAATTGG AGCAGATGAC AACTACTGCT GAAAACTGGT TGAAAACCCA GCCTACCACC 
ACATCAGAGC CAACAGCAAT TAAAAGCCAG TTAAAAATTT GTAAGGATGA AATCAACCGA 
CTGTCAGCTC TTCAGCCTCA AATCGAGCGA TTAAAAATTC AAAGCATAGC CCTGAAAGAG 
AAAGGACAAG GGCCAATGTT CCTGGATGCA GACTTTGTGG CCTTTACAAA TCATTTTAAC 
CAAGTCTTTG CTGATGTGCA GGCAAGAGAA AAAGAGCTAC AAACAATTTT TGACAGTTTG 
CCACCCATGC GCTATCAGGA GACTATGAGT ACCATCCTGA CATGGATCCA GCAGTCAGAA 
ACCAAACTCT CTATACCTCA GGTTACTGTC ACTGAATATG ACATCATGGA ACAGAGACTC 
GGAGAGCTAC AGGCTTTACA AAGCTCTCTG CAAGAGCAAC AAAATGGCCT AAACTATCTC 
AGCACCACTG TGAAAGAGAT GTCAAAGAAA GCACCACTGT CTGATATTAG TCGGAAATAT 
CAATCAGAAT TTGAAGAGAT TGAGGGACGT TGGAAGAAGC TGTCTTCCCA GCTGGTTGAA 
CATTGTCAAA AGTTGGAGGA GCAAATGGCT AAACTTCGAA AAATTCAGAA TCACATAAAA 
ACTCTGAAGA AATGGATCAC TGAAGTCGAT GTTTTCCTGA AGGAGGAATG GCCTGCCCTT 
GGGGATTCAG AAATTCTGAA AAGACAGCTG AAACAGTGCA GGCTTTTAGT CAATGACATT 
CAGACCATCC AGCCTAGTCT CAACAGTGTC AATGAAGGGG CTCAGAAGAT GAAGAATGAA 
GCAGAACCAG AGTTTGCTGG CAGACTTGAG ACAGAGCTCC GAGAACTTAA CACCCAGTGG 
GATTACATGT GCCGCCAGGT CTATGCCAGG AAGGAAGCCT TAAAAGGAGG TTTGGATAAA 
ACTGTAAGTC TTCAGAAAGA TCTGTCAGAG ATGCATGAGT GGATGACACA AGCTGAAGAA 
GAATACCTAG AGAGAGATTT CGAATACAAG ACCCCTGATG AATTACAGAC AGCAGTTGAA 
GAGATGAAGA GAGCTAAAGA AGAGGCCCAG CAAAAAGAAG CAAAAGTGAA ACTCCTAACC 
GAGTCCGTCA ATAGTGTCAT AGCTCAGGCT CCACCTGCAG CACAAGAGGC CTTAAAAAAG 
GAACTTGACA CTCTCACCAC CAACTACCAG TGGCTCTGCA CCAGGCTCAA TGGCAAATGC 
AAGACCTTGG AAGAAGTTTG GGCGTGCTGG CATGAGTTAT TGTCCTACTT GGAGAAGGCA 
AACAAGTGGC TAAGTGAAGT AGAAGTCAAG CTTAAAACCA CTGAAAATAT TTCTGGGGGA 
GCTGAGGAAA TCGCCGAGGT GCTTGATTCG CTTGAAAATT TGATGCAACA TTCAGAGGAT 
AACCCGAATC AGATTCGCAT ATTGGCACAG ACCTTGACAG ATGGTGGAGT CATGGATGAA 
CTGATCAATG AGGAGCTTGA GACATTTAAT TCTCGTTGGA GAGAACTCCA TGAAGAGGCT 
GTGAGGAGGC AAAAGTTGCT TGAGCAGAGT ATCCAGTCGG CCCAGGAGAT AGAAAAATCC 
TTGCACTTAA TTCAGGAGTC CCTCTCTTCC ATTGACAAGC AGTTGGCAGC TTATATTGCT 
GACAAAGTGG ATGCAGCTCA GATGCCTCAG GAAGCCCAGA AAATCCAATC AGATTTGACA 
AGTCATGAGA TCAGTTTAGA AGAAATGAAG AAACATAACC AGGGAAAGGA GACTGCCCAA 
AGGGTACTAT CCCAAATTGA TGTGGCACAG AAAAAATTGC AGGATGTTTC CATGAAGTTT 
CGATTATTCC AGAAGCCAGC CAATTTTGAG CAGCGCCTAC AAGAAAGTAA AATGATTTTA 
GATGAAGTGA AGATGCATTT ACCTGCGTTG GAAACAAAGA GTGTGGAACA GGAAGTAGTA 
CAGTCACAGT TAAATCATTG TGTGAACTTG TATAAAAGTC TGAGTGAAGT GAAGTCTGAA 
GTGGAAATGG TAATAAAAAC TGGACGTCAG ATTGTACAGA AGAAGCAGAC GGAAAACCCG 
AAAGAGCTTG ATGAAAGAGT TACAGCTTTG AAATTGCATT ATAATGAGCT GGGAGCAAAG 
GTGACAGAAA GAAAGCAACA GTTGGAAAAA TGCTTGAAAT TGTCCCGTAA GATGCGAAAG 
GAAATGAATG CCCTGACAGA ATGGCTGGCA GCGACAGATA TGGAACTGAC GAAGAGATCG 
GCAGTTGAAG GAATGCCTAG TAATTTGGAT TCTGAAGTTG CCTGGGGAAA GGCTACTCAG 
AAAGAGATTG AGAAACAGAA GGTTCACCTA AAGAGTGTCA CAGAGGTAGG AGAGGCCTTG 
AAAACGGTTT TGGGCAAGAA GGAAATGTTG GTGGAAGATA AACTGAGTCT TCTGAATAGT 
AACTGGATAG CCGTCACTTC CCGAGCAGAA GAGTGGTTAA ACCTTTTATT GGAATACCAG 
AAACACATGG AAAACTTTGA CCAGAATGTG GATTACATCA CAAACTGGAT CATTCAGGCT 
GATGCACTTT TGGATGAATC TGAGAAAAAG AAACCTCAGC AAAAAGAAGA CATACTTAAG 
CGTTTAAAGG CTGAAATGAA TGACATACGT CCAAAGGTGG ATTCTACACG TGACCAAGCA 
GCAAACCTGA TGGCAAACCG CGGCGACCAC TGCAGGAAAG TAGTAGAGCC CAAAATCTCA 
GAGCTCAACC ATCGATTTGC AGCCATTTCT CACAGAATTA AGACTGGAAA GGCCTCCATT 
CCTTTGAAGG AATTGGAGCA GTTTAACTCA GATATACAAA AATTGCTTGA ACCACTGGAG 
GCTGAAATTC AGCAGGGGGT GAATCTGAAA GAGGAAGACT TCAATAAAGA TATGAGTGAA 
GACAATGAGG GTACTGTAAA AGAATTGTTG CAAAGAGGAG ACAACTTACA ACAAAGAATC 
ACAGATGAGA GAAAGCGAGA GGAAATAAAG ATAAAACAAC AGCTGTTACA GACAAAACAT 
AATGCTCTCA AGGATTTGAG GTCTCAAAGA AGAAAAAAGG CTCTAGAAAT TTCTCACCAG 
TGGTATCAGT ACAAGAGGCA GGCTGATGAT CTCCTGAAAT GCTTGGATGA CATTGAAAAA 
AAATTAGCCA GCCTACCTGA ACCCAGAGAT GAAAGGAAAA TAAAGGAAAT TGATCGTGAA 
TTGCAGAAGA AGAAAGAGGA GCTGAATGCA GTGCGTAGGC AAGCTGAGGG CTTGTCTGAG 
GATGGGGCCG CAATGGCAGT GGAGCCAACT CAGATCCAGC TCAGCAAGCG CTGGCGGGAA 
ATTGAGAGCA AATTTGCTCA GTTTCGAAGA CTCAACTTTG CACAAATTCA CACTGTCCAT 
GAAGAGTCAG TGGTGGCGAT GACTGAAGAC ATGCCTTTGG AAATTTCTTA TGTGCCTTCT 
ACTTACCTGA CTGAGATCAC TCATGTCTCA CAAGCCCTAT CAGAAGTGGA AGAGCTTCTT 
AATGCTCCCG ACCTTTGTGC TCAAGATTTT GAAGATCTCT TTAAACAAGA GGAATCCTTG 
AAGAACATAA AAGACAGCCT GCAACAAATC TCAGGTCGGA TTGACATCAT TCACAATAAA 
AAGACAGCAG CATTGCACAG TGCCACTCCT GCAGAAAGGG CAAAGCTCCA GGAAGCTCTC 
TCACGGCTTG ATTTCCAATG GGAAAGAGTT AACAATATGT ACAAGGACCG ACAAGGGAGA 
TTTGACAGAT CTGTGGAAAA ATGGCGGCGG TTTCATTATG ATATGAAGAT ACTTAATCAA 
TGGCTAACAG AAGCTGAACA GTTTCTCAAA AAGACACAAA TTCCTGAGAA TTGGGAACAT 
GCCAAATACA AATGGTATCT TAAGGAACTC CAGGATGGCA TTGGACAGCG GCAAAGTGTT 
GTCAGGGTAT TGAATGCAAC TGGGGAAGAA ATAATTCAAC AGTCCTCAAA AACAGATGCC 
AGTATTCTCC AAGAAAAACT GGGAAGCCTG AATCTGCGGT GGCAGGAGGT CTGCAAACAG 
CTGGCAGAAA GAAAAAAGAG GCTAGAGGAA CAGAAGAATA TCTTGTCAGA ATTTCAAAGA 
GATGTAAATG AATTTGTTTT ATGGTTGGAA GAAGCGGATA ACGTTGCTAA TATTCCACTT 
GAACCTGGAA ATGAGCAGCA GCTAAAAGAA AAACTTGAAC AAGTCAAGTT ACTGGCAGAA 
GAGTTGCCCC TGCGCCAGGG AATTCTAAAA CAATTAAATG AAACTGGAGG AACAGTGCTT 
GTAAGTGCTC CCCTAAGCCC AGAAGAGCAA GATAAACTTG AAAATAAGCT CAAGCAGACA 
AATCTTCAGT GGATAAAGGT TTCTAGAAAT CTGCCTGAGA AGCAAGAAGA AATTGAGGCA 
CACGTAAAAG ACCTTGGACA GCTGGAAGAG CAGTTAAATC ATCTGCTTCT ATGGCTGTCT 
CCTATTAGGA ATCAGTTGGA AATTTACAAT CAGCCAAATC AAACAGGACC ATTTGACATC 
AAGGAAATTG AAGTAGCAGT TCAAGCTAAA CAGCCGGATG TGGAAGGGAT TTTGTCTAAA 
GGGCAGCATT TGTACAAGGA AAAACCAGCC ACTCAGCCAG CGAAGAGAAA GCTGGAAGAT 
CTCAGCTCTG ATTGGAAGGT GGTAACTCAG TTGCTTCAAG AGCTGCGGGC AAAGCAGCCT 
GGCCCAGCTC CTGGACTGAC CACTGTCAGA GCCCCTCCCA GTCAGACTGT TACTCTGGTG 
ACACAACCCG CGGTTACCAA GGAAACTGCC ATCTCCAAAC TAGAAATGCC ATCTTCATTG 
CTGTTGGAGG TACCTGCACT GGCAGATTTC AACCGAGCTT GGACAGAACT TACCGACTGG 
CTGTCTCTGC TTGATCGAGT TATAAAATCA CAGAGGGTGA TGGTGGGTGA TCTTGAAGAC 
ATTAACGAGA TGATCATCAA GCAGAAGGCA ACGCTGCAGG ATTTGGAACA GAGGCGCCCC 
CAGTTGGAAG AACTCATTAC CGCTGCCCAG AATTTGAAAA ACAAGACCAG CAATCAAGAG 
GCTAGAACAA TCATTACTGA TCGAATTGAA AGAATTCAGA GTCAGTGGGA TGAAGTACAG 
GAACATCTTC AGAACCGGAG GCAACAGTTG AATGAAATGT TAAAGGATTC CACACAATGG 
CTGGAAGCTA AAGAGGAGGC TGAGCAGGTG TTGGGGCAGG CCAGAGCCAA GCTTGAGTCA 
TGGAAGGAGG CTCCCTACAC AGTAGATGCA ATCCAAAAGA AAATCACAGA AACCAAGTTG 
GCCAAAGACC TCCGCCAGTG GCAGATAAAT GTAGATGTTG CAAATGATTT GGCACTGAAA 
CTTCTCCGAG ATTATTCTGC AGATGATACC AGAAAAGTAC ACATGATAAC AGAGAACATC 
AATGCCTCTT GGGCAAGCAT CCATAAAAGA TTGAGTGAGC GAGAGGCTGC TCTGGAAGAA 
ACCCACAGAT TACTGCAACA GTTCCCCTTG GACCTGGAGA AGTTCCTTGC CTGGCTTACA 
GAAGCCGAAA CAACTGCCAA CGTCCTGCAG GATGCCACCC ATAAGGAAAG GCTTCTAGAA 
GATTCCAAGG GAGTAAGAGA GCTGATGAAA CAATGGCAAG ATCTCCAAGG AGAAATCGAA 
GCTCACACAG ATATCTATCA CAACCTGGAC GAAAATGGCC AAAAAGTCCT GAGATCCCTG 
GAAGGTTCTG ACGATGCAGC CTTGTTGCAA AGACGTTTGG ATAACATGAA CTTCAAGTGG 
AGCGAACTTC GGAAAAAGTC TCTCAACATT AGGTCTCACT TGGAAGCCAG TTCTGACCAG 
TGGAAGCGTC TGCACCTTTC TCTTCAGGAA CTTCTGGTAT GGCTCCAGCT GAAAGATGAT 
GAGTTAAGCC GGCAGGCACC CATTGGAGGA GACTTTCCAG CGGTGCAGAA GCAGAATGAT 
GTACACAGGG CCTTCAAGAG GGAATTGAAA ACGAAAGAAC CTGTAATCAT GAGTACTCTT 
GAGACTGTAC GAATATTTCT GACAGAGCAG CCTTTAGAAG GACTAGAGAA ACTCTACCAG 
GAGCCCAGAG AGCTGCCTCC TGAAGAGAGA GCCCAGAATG TCACACGGCT CCTACGAAAG 
CAAGCTGAGG AGGTCAACAC TCAGTGGGAA AAACTGAACG TGCACTCTGC AGACTGGCAG 
AGAAAAATAG ACGAGGCCCT CGAAAGACTC CAGGAGCTTC AGGAAGCAAC AGATGAGCTG 
GATCTCAAAC TACGTCAGGC AGAGGTGATC AAAGGATCCT GGCAGCCTGT GGGTGATCTC 
CTCATTGACT CTCTCCAAGA TCACCTCGAA AAAGTCAAGG CGCTTCGAGG AGAAATTACA 
CCTCTGAAAG AGAATGTCAG CTACGTCAAT GACCTTGCTC GCCAACTCAC TACGTTGGGC 
ATTCAGCTGT CACCATATAA CCTCAACACT CTGGAAGACC TGAACACCAG ATGGAAGCTT 
CTGCAGGTGG CCATTGAGGA CCGCATCAGG CAGCTGCATG AAGCGCACAG GGACTTTGGA 
CCAGCCTCCC AGCACTTCCT TTCCACTTCT GTCCAGGGTC CCTGGGAGAG AGCCATCTCA 
CCAAACAAAG TGCCCTACTA TATCAACCAC GAGACCCAAA CAACTTGCTG GGACCATCCC 
AAAATGACAG AGCTCTACCA GTCTTTAGCT GACCTGAATA ATGTCAGGTT CTCAGCTTAC 
AGGACTGCCA TGAAACTCCG AAGACTGCAG AAGGCCCTTT GCTTGGATCT CTTGAGCCTA 
TCGGCTGCAT GCGATGCCTT GGACCAGCAC AACCTCAAGC AAAATGACCA GCCCATGGAT 
ATCCTGCAGG TCATTAACTG TCTGACCACT ATTTATGATC GCCTAGAGCA AGAGCACAAC 
AATCTGGTCA ACGTCCCTCT CTGCGTGGAT ATGTGTCTCA ATTGGCTGCT GAATGTTTAT 
GACACGGGAC GAACGGGGAG GATCCGGGTC CTGTCTTTTA AAACTGGCAT CATTTCTCTG 
TGTAAAGCCC ATTTGGAAGA CAAGTACAGA TACCTCTTCA AGCAAGTGGC AAGTTCGACA 
GGATTTTGTG ACCAGCGCAG GCTGGGCCTC CTCCTGCATG ACTCTATCCA GATCCCAAGA 
CAGTTGGGTG AAGTCGCATC GTTCGGGGGC AGTAACATTG AGCCGAGTGT CAGGAGCTGC 
TTCCAGTTTG CTAATAATAA GCCTGAGATC GAAGCGGCCC TCTTCCTAGA CTGGATGCGC 
CTGGAGCCCC AGTCCATGGT GTGGCTGCCT GTCCTGCACC GAGTGGCTGC CGCGGAAACT 
GCCAAGCACC AGGCCAAGTG CAACATCTGC AAGGAGTGTC CCATCATCGG ATTCAGGTAC 
AGGAGTCTAA AGCACTTTAA TTATGACATC TGCCAAAGTT GCTTTTTTTC TGGTCGAGTT 
GCAAAAGGCC ATAAAATGCA CTATCCCATG GTGGAATACT GCACTCCGAC TACATCGGGA 
GAAGATGTCC GTGACTTTGC CAAGGTACTA AAAAACAAAT TTCGAACCAA AAGGTATTTT 
GCGAAGCATC CCCGAATGGG CTACCTGCCA GTGCAGACTG TCTTAGAGGG GGACAACATG 
GAAACTCCTG TCACTCTGAT CAACTTCTGG CCGGTAGATT CTGCGCCTGC CTCGTCCCCT 
CAGCTTTCAC ACGATGATAC TCATTCACGC ATTGAGCATT ATGCTAGCAG GCTAGCAGAA 
ATGGAAAACA GCAATGGATC TTATCTAAAT GATAGCATCT CTCCTAATGA GAGCATAGAT 
GATGAACATT TGTTAATCCA GCATTACTGC CAAAGTTTGA ACCAGGACTC CCCCCTGAGC 
CAGCCTCGTA GTCCTGCCCA GATCTTGATT TCCTTAGAGA GTGAGGAAAG AGGGGAGCTA 
GAGAGAATCC TAGCAGATCT TGAGGAAGAA AACAGAAATC TGCAAGCAGA GTATGATCGT 
CTAAAGCAGC AGCATGAACA CAAAGGCCTG TCCCCACTGC CATCCCCTCC TGAAATGATG 
CCTACTTCTC CCCAAAGTCC CCGGGATGCT GAGCTCATCG CTGAGGCCAA GCTGCTGCGT 
CAACACAAAG GCCGCCTGGA AGCCAGGATG CAAATCCTTG AAGACCATAA CAAACAACTG 
GAATCCCAGT TACACAGGCT CAGGCAGCTG CTGGAGCAAC CCCAGGCAGA GGCCAAGGTG 
AATGGTACAA CGGTGTCTTC TCCTTCTACC TCTCTTCAGA GGTCAGATAG CAGTCAGCCT 
ATGCTGCTCC GGGTGGTCGG CAGTCAGACT TCAGAATCCA TGGGCGAGGA AGACCTGCTC 
AGCCCTCCCC AGGACACAAG CACAGGGTTA GAGGAAGTGA TGGAGCAGCT CAACCACTCC 
TTCCCTAGTT CCAGAGGACA CAATGTAGGA AGCCTTTTCC ACTTGGCAGA TGATTTGGGC 
AGAGCGATGG AGTCCTTAGT TTCAGTCATG ACAGATGAAG AAGGGGCAGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMD ( XM_014111342.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013966817.1 Canis lupus familiaris dystrophin (DMD), transcript variant X2, mRNA.

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CloneID	OCe45413
Clone ID Related Accession (Same CDS sequence)	XM_014111342.1 , XM_014111342.2
Accession Version	XM_014111342.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11094bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	dystrophin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014111342.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTGGT GGGAAGAAGT AGAGGACTGT TATGAAAGAG AAGATGTTCA AAAGAAAACA 
TTCACAAAAT GGATAAATGC ACAGTTTTCT AAGTTTGGGA AGCAGCACAT AGAGAACCTC 
TTCAGTGACC TACAGGATGG GAGACGCCTC CTAGACCTTT TGGAAGGCCT GACAGGGCAA 
AAACTGCCAA AAGAAAAAGG ATCCACAAGA GTTCATGCCC TGAACAATGT CAACAAGGCA 
CTGCGCGTCT TGCAGAAAAA TAATGTTGAT TTAGTGAACA TTGGAAGTAC TGACATAGTA 
GATGGAAATC ACAAACTGAC TCTTGGTTTG ATTTGGAATA TAATCCTCCA CTGGCAGGTC 
AAAAATGTAA TGAAAAATAT CATGGCTGGA TTGCAACAAA CCAACAGTGA AAAGATTCTC 
CTGAGCTGGG TCCGACAATC AACTCGTAAT TATCCACAGG TTAATGTCAT TAACTTCACC 
ACCAGCTGGT CTGATGGCCT GGCTTTGAAC GCTCTCATCC ACAGTCATAG GCCAGACCTG 
TTTGATTGGA ATAGTGTGGT TTGCCAGCAG TCAGCCACAC AACGCCTGGA ACATGCATTC 
AACATTGCCA AATATCAATT AGGCATAGAG AAACTGCTTG ATCCTGAAGA TGTTGCCACC 
ACTTATCCAG ATAAGAAGTC CATCTTAATG TATATCACAT CACTCTTCCA AGTTTTGCCT 
CAACAAGTGA GCATTGAAGC CATCCAGGAA GTGGAAATGT TGCCAAGGCC ATCAAAAGTT 
ACTAGAGAAG AACATTTTCA ATTACATCAT CAAATGCACT ATTCTCAACA GATCACAGTC 
AGTCTAGCAC AGGGCTATGA ACGAGCCCCT TCCTCTCCTA AGCCTCGGTT CAAGAGCTAT 
GCCTACACAC AGGCTGCTTA TGTCACCACT TCTGACCCCA CACGGAGCCC ACTTCCTTCA 
CAGCATTTGG AAACTCCTGA AGACAAGTCA TTTGGCCGGT CATTGACAGA GACCGAAGCA 
AACCTGGACA GTTATCAAAC AGCTTTGGAA GAAGTACTCT CGTGGCTTCT TTCAGCTGAG 
GATGCACTGC AAGCCCAAGG AGAGATTTCT AATGATGTCG AAGAAGTGAA AGAACAATTT 
CATACTCATG AGGGATATAT GATGGACTTG ACATCCCATC AGGGACGGGT CGGTAATGTT 
CTCCAACTGG GAAGTCAACT GATTGGAACA GGGAAATTAT CAGAAGATGA AGAAACCGAA 
GTGCAGGAAC AAATGAATCT CCTCAATTCA AGATGGGAAT GCCTCAGGGT AGCTAGCATG 
GAAAAACAAA GCAATTTACA TAAAGTTCTA ATGGATCTCC AGAATCAGCA ACTGAAAGAG 
TTAAATGACT GGCTAACCAA AACAGAAGAG AGAACAAGGA AAATGGAGAA GGAGCCCCTT 
GGACCTGATA TTGAAGACCT AAAACGCCAA GTACAACAAC ATAAGGTGCT TCAAGAAGAC 
TTAGAACAGG AACAAGTCAG GGTCAATTCC CTCACTCATA TGGTGGTGGT AGTCGATGAA 
TCTAGTGGAG ACCATGCAAC TGCTGCTTTG GAAGAACAAC TTAAGGTACT GGGAGATCGA 
TGGGCAAACA TCTGTAGGTG GACAGAAGAT CGCTGGGTTC TTTTACAAGA CATCCTCCTA 
AAATGGCAGC GTTTTACTGA AGAACAGTGC CTTTTCAGTG CATGGCTTTC GGAGAAGGAA 
GATGCAGTGA ACAAGATTCA CACAACTGGC TTTAAGGATC AAAGTGAAGT GTTATCAAAT 
CTTCAGAAAC TGGCTGTCTT AAAAACAGAT CTGGAAAAGA AGAAGCAAAC CATGGACAAA 
CTCTGCTCAC TCAACCAAGA CCTTCTTTCA GCGCTGAAAA ACACAGTGGT AGCCCACAAG 
ATGGAAGCAT GGCTGGACAA CTTTGCCCAG CGCTGGGATA ATTTAGTCCA GAAACTTGAA 
AAAAGTTCAG CACAGATTTC ACAGGCTGTC ACCACCACTC AGCCATCACT AACACAGACA 
ACTGTAATGG AAACAGTAAC TATGGTGACC ACGAGGGAAC ACATCTTGGT AAAGCATGCC 
CAAGAGGAAC TGCCACCACC ACCCCCTCAG AAGAAGAGGC AGATTATCGT GGATTCTGAA 
ATTAGGAAAA GGTTGGATGT CGATATAACT GAACTTCACA GTTGGATTAC TCGTTCAGAA 
GCTGTGTTGC AGAGTCCTGA ATTTGCAATC TATCGGAAGG AAGGCAACTT CTCAGACCTT 
AAAGAAAAAG TCAATGCCAT AGAGCGAGAA AAAGCCGAGA AGTTCAGAAA ACTGCAAGAT 
GCCAGCAGAT CAGCTCAGGC CCTGGTGGAA CAGATGGTGA ATGAGGGTGT TAATGCTGAC 
AGCATCAAAC AAGCCTCCGA ACAACTGAAC AGCCGGTGGA TAGAGTTCTG CCAATTGCTA 
AGCGAGAGAC TTAACTGGCT GGAGTATCAG AACAACATCA TCACTTTCTA TAATCAGCTA 
CAACAATTGG AGCAGATGAC AACTACTGCT GAAAACTGGT TGAAAACCCA GCCTACCACC 
ACATCAGAGC CAACAGCAAT TAAAAGCCAG TTAAAAATTT GTAAGGATGA AATCAACCGA 
CTGTCAGCTC TTCAGCCTCA AATCGAGCGA TTAAAAATTC AAAGCATAGC CCTGAAAGAG 
AAAGGACAAG GGCCAATGTT CCTGGATGCA GACTTTGTGG CCTTTACAAA TCATTTTAAC 
CAAGTCTTTG CTGATGTGCA GGCAAGAGAA AAAGAGCTAC AAACAATTTT TGACAGTTTG 
CCACCCATGC GCTATCAGGA GACTATGAGT ACCATCCTGA CATGGATCCA GCAGTCAGAA 
ACCAAACTCT CTATACCTCA GGTTACTGTC ACTGAATATG ACATCATGGA ACAGAGACTC 
GGAGAGCTAC AGGCTTTACA AAGCTCTCTG CAAGAGCAAC AAAATGGCCT AAACTATCTC 
AGCACCACTG TGAAAGAGAT GTCAAAGAAA GCACCACTGT CTGATATTAG TCGGAAATAT 
CAATCAGAAT TTGAAGAGAT TGAGGGACGT TGGAAGAAGC TGTCTTCCCA GCTGGTTGAA 
CATTGTCAAA AGTTGGAGGA GCAAATGGCT AAACTTCGAA AAATTCAGAA TCACATAAAA 
ACTCTGAAGA AATGGATCAC TGAAGTCGAT GTTTTCCTGA AGGAGGAATG GCCTGCCCTT 
GGGGATTCAG AAATTCTGAA AAGACAGCTG AAACAGTGCA GGCTTTTAGT CAATGACATT 
CAGACCATCC AGCCTAGTCT CAACAGTGTC AATGAAGGGG CTCAGAAGAT GAAGAATGAA 
GCAGAACCAG AGTTTGCTGG CAGACTTGAG ACAGAGCTCC GAGAACTTAA CACCCAGTGG 
GATTACATGT GCCGCCAGGT CTATGCCAGG AAGGAAGCCT TAAAAGGAGG TTTGGATAAA 
ACTGTAAGTC TTCAGAAAGA TCTGTCAGAG ATGCATGAGT GGATGACACA AGCTGAAGAA 
GAATACCTAG AGAGAGATTT CGAATACAAG ACCCCTGATG AATTACAGAC AGCAGTTGAA 
GAGATGAAGA GAGCTAAAGA AGAGGCCCAG CAAAAAGAAG CAAAAGTGAA ACTCCTAACC 
GAGTCCGTCA ATAGTGTCAT AGCTCAGGCT CCACCTGCAG CACAAGAGGC CTTAAAAAAG 
GAACTTGACA CTCTCACCAC CAACTACCAG TGGCTCTGCA CCAGGCTCAA TGGCAAATGC 
AAGACCTTGG AAGAAGTTTG GGCGTGCTGG CATGAGTTAT TGTCCTACTT GGAGAAGGCA 
AACAAGTGGC TAAGTGAAGT AGAAGTCAAG CTTAAAACCA CTGAAAATAT TTCTGGGGGA 
GCTGAGGAAA TCGCCGAGGT GCTTGATTCG CTTGAAAATT TGATGCAACA TTCAGAGGAT 
AACCCGAATC AGATTCGCAT ATTGGCACAG ACCTTGACAG ATGGTGGAGT CATGGATGAA 
CTGATCAATG AGGAGCTTGA GACATTTAAT TCTCGTTGGA GAGAACTCCA TGAAGAGGCT 
GTGAGGAGGC AAAAGTTGCT TGAGCAGAGT ATCCAGTCGG CCCAGGAGAT AGAAAAATCC 
TTGCACTTAA TTCAGGAGTC CCTCTCTTCC ATTGACAAGC AGTTGGCAGC TTATATTGCT 
GACAAAGTGG ATGCAGCTCA GATGCCTCAG GAAGCCCAGA AAATCCAATC AGATTTGACA 
AGTCATGAGA TCAGTTTAGA AGAAATGAAG AAACATAACC AGGGAAAGGA GACTGCCCAA 
AGGGTACTAT CCCAAATTGA TGTGGCACAG AAAAAATTGC AGGATGTTTC CATGAAGTTT 
CGATTATTCC AGAAGCCAGC CAATTTTGAG CAGCGCCTAC AAGAAAGTAA AATGATTTTA 
GATGAAGTGA AGATGCATTT ACCTGCGTTG GAAACAAAGA GTGTGGAACA GGAAGTAGTA 
CAGTCACAGT TAAATCATTG TGTGAACTTG TATAAAAGTC TGAGTGAAGT GAAGTCTGAA 
GTGGAAATGG TAATAAAAAC TGGACGTCAG ATTGTACAGA AGAAGCAGAC GGAAAACCCG 
AAAGAGCTTG ATGAAAGAGT TACAGCTTTG AAATTGCATT ATAATGAGCT GGGAGCAAAG 
GTGACAGAAA GAAAGCAACA GTTGGAAAAA TGCTTGAAAT TGTCCCGTAA GATGCGAAAG 
GAAATGAATG CCCTGACAGA ATGGCTGGCA GCGACAGATA TGGAACTGAC GAAGAGATCG 
GCAGTTGAAG GAATGCCTAG TAATTTGGAT TCTGAAGTTG CCTGGGGAAA GGCTACTCAG 
AAAGAGATTG AGAAACAGAA GGTTCACCTA AAGAGTGTCA CAGAGGTAGG AGAGGCCTTG 
AAAACGGTTT TGGGCAAGAA GGAAATGTTG GTGGAAGATA AACTGAGTCT TCTGAATAGT 
AACTGGATAG CCGTCACTTC CCGAGCAGAA GAGTGGTTAA ACCTTTTATT GGAATACCAG 
AAACACATGG AAAACTTTGA CCAGAATGTG GATTACATCA CAAACTGGAT CATTCAGGCT 
GATGCACTTT TGGATGAATC TGAGAAAAAG AAACCTCAGC AAAAAGAAGA CATACTTAAG 
CGTTTAAAGG CTGAAATGAA TGACATACGT CCAAAGGTGG ATTCTACACG TGACCAAGCA 
GCAAACCTGA TGGCAAACCG CGGCGACCAC TGCAGGAAAG TAGTAGAGCC CAAAATCTCA 
GAGCTCAACC ATCGATTTGC AGCCATTTCT CACAGAATTA AGACTGGAAA GGCCTCCATT 
CCTTTGAAGG AATTGGAGCA GTTTAACTCA GATATACAAA AATTGCTTGA ACCACTGGAG 
GCTGAAATTC AGCAGGGGGT GAATCTGAAA GAGGAAGACT TCAATAAAGA TATGAGTGAA 
GACAATGAGG GTACTGTAAA AGAATTGTTG CAAAGAGGAG ACAACTTACA ACAAAGAATC 
ACAGATGAGA GAAAGCGAGA GGAAATAAAG ATAAAACAAC AGCTGTTACA GACAAAACAT 
AATGCTCTCA AGGATTTGAG GTCTCAAAGA AGAAAAAAGG CTCTAGAAAT TTCTCACCAG 
TGGTATCAGT ACAAGAGGCA GGCTGATGAT CTCCTGAAAT GCTTGGATGA CATTGAAAAA 
AAATTAGCCA GCCTACCTGA ACCCAGAGAT GAAAGGAAAA TAAAGGAAAT TGATCGTGAA 
TTGCAGAAGA AGAAAGAGGA GCTGAATGCA GTGCGTAGGC AAGCTGAGGG CTTGTCTGAG 
GATGGGGCCG CAATGGCAGT GGAGCCAACT CAGATCCAGC TCAGCAAGCG CTGGCGGGAA 
ATTGAGAGCA AATTTGCTCA GTTTCGAAGA CTCAACTTTG CACAAATTCA CACTGTCCAT 
GAAGAGTCAG TGGTGGCGAT GACTGAAGAC ATGCCTTTGG AAATTTCTTA TGTGCCTTCT 
ACTTACCTGA CTGAGATCAC TCATGTCTCA CAAGCCCTAT CAGAAGTGGA AGAGCTTCTT 
AATGCTCCCG ACCTTTGTGC TCAAGATTTT GAAGATCTCT TTAAACAAGA GGAATCCTTG 
AAGAACATAA AAGACAGCCT GCAACAAATC TCAGGTCGGA TTGACATCAT TCACAATAAA 
AAGACAGCAG CATTGCACAG TGCCACTCCT GCAGAAAGGG CAAAGCTCCA GGAAGCTCTC 
TCACGGCTTG ATTTCCAATG GGAAAGAGTT AACAATATGT ACAAGGACCG ACAAGGGAGA 
TTTGACAGAT CTGTGGAAAA ATGGCGGCGG TTTCATTATG ATATGAAGAT ACTTAATCAA 
TGGCTAACAG AAGCTGAACA GTTTCTCAAA AAGACACAAA TTCCTGAGAA TTGGGAACAT 
GCCAAATACA AATGGTATCT TAAGGAACTC CAGGATGGCA TTGGACAGCG GCAAAGTGTT 
GTCAGGGTAT TGAATGCAAC TGGGGAAGAA ATAATTCAAC AGTCCTCAAA AACAGATGCC 
AGTATTCTCC AAGAAAAACT GGGAAGCCTG AATCTGCGGT GGCAGGAGGT CTGCAAACAG 
CTGGCAGAAA GAAAAAAGAG GCTAGAGGAA CAGAAGAATA TCTTGTCAGA ATTTCAAAGA 
GATGTAAATG AATTTGTTTT ATGGTTGGAA GAAGCGGATA ACGTTGCTAA TATTCCACTT 
GAACCTGGAA ATGAGCAGCA GCTAAAAGAA AAACTTGAAC AAGTCAAGTT ACTGGCAGAA 
GAGTTGCCCC TGCGCCAGGG AATTCTAAAA CAATTAAATG AAACTGGAGG AACAGTGCTT 
GTAAGTGCTC CCCTAAGCCC AGAAGAGCAA GATAAACTTG AAAATAAGCT CAAGCAGACA 
AATCTTCAGT GGATAAAGGT TTCTAGAAAT CTGCCTGAGA AGCAAGAAGA AATTGAGGCA 
CACGTAAAAG ACCTTGGACA GCTGGAAGAG CAGTTAAATC ATCTGCTTCT ATGGCTGTCT 
CCTATTAGGA ATCAGTTGGA AATTTACAAT CAGCCAAATC AAACAGGACC ATTTGACATC 
AAGGAAATTG AAGTAGCAGT TCAAGCTAAA CAGCCGGATG TGGAAGGGAT TTTGTCTAAA 
GGGCAGCATT TGTACAAGGA AAAACCAGCC ACTCAGCCAG CGAAGAGAAA GCTGGAAGAT 
CTCAGCTCTG ATTGGAAGGT GGTAACTCAG TTGCTTCAAG AGCTGCGGGC AAAGCAGCCT 
GGCCCAGCTC CTGGACTGAC CACTGTCAGA GCCCCTCCCA GTCAGACTGT TACTCTGGTG 
ACACAACCCG CGGTTACCAA GGAAACTGCC ATCTCCAAAC TAGAAATGCC ATCTTCATTG 
CTGTTGGAGG TACCTGCACT GGCAGATTTC AACCGAGCTT GGACAGAACT TACCGACTGG 
CTGTCTCTGC TTGATCGAGT TATAAAATCA CAGAGGGTGA TGGTGGGTGA TCTTGAAGAC 
ATTAACGAGA TGATCATCAA GCAGAAGGCA ACGCTGCAGG ATTTGGAACA GAGGCGCCCC 
CAGTTGGAAG AACTCATTAC CGCTGCCCAG AATTTGAAAA ACAAGACCAG CAATCAAGAG 
GCTAGAACAA TCATTACTGA TCGAATTGAA AGAATTCAGA GTCAGTGGGA TGAAGTACAG 
GAACATCTTC AGAACCGGAG GCAACAGTTG AATGAAATGT TAAAGGATTC CACACAATGG 
CTGGAAGCTA AAGAGGAGGC TGAGCAGGTG TTGGGGCAGG CCAGAGCCAA GCTTGAGTCA 
TGGAAGGAGG CTCCCTACAC AGTAGATGCA ATCCAAAAGA AAATCACAGA AACCAAGTTG 
GCCAAAGACC TCCGCCAGTG GCAGATAAAT GTAGATGTTG CAAATGATTT GGCACTGAAA 
CTTCTCCGAG ATTATTCTGC AGATGATACC AGAAAAGTAC ACATGATAAC AGAGAACATC 
AATGCCTCTT GGGCAAGCAT CCATAAAAGA TTGAGTGAGC GAGAGGCTGC TCTGGAAGAA 
ACCCACAGAT TACTGCAACA GTTCCCCTTG GACCTGGAGA AGTTCCTTGC CTGGCTTACA 
GAAGCCGAAA CAACTGCCAA CGTCCTGCAG GATGCCACCC ATAAGGAAAG GCTTCTAGAA 
GATTCCAAGG GAGTAAGAGA GCTGATGAAA CAATGGCAAG ATCTCCAAGG AGAAATCGAA 
GCTCACACAG ATATCTATCA CAACCTGGAC GAAAATGGCC AAAAAGTCCT GAGATCCCTG 
GAAGGTTCTG ACGATGCAGC CTTGTTGCAA AGACGTTTGG ATAACATGAA CTTCAAGTGG 
AGCGAACTTC GGAAAAAGTC TCTCAACATT AGGTCTCACT TGGAAGCCAG TTCTGACCAG 
TGGAAGCGTC TGCACCTTTC TCTTCAGGAA CTTCTGGTAT GGCTCCAGCT GAAAGATGAT 
GAGTTAAGCC GGCAGGCACC CATTGGAGGA GACTTTCCAG CGGTGCAGAA GCAGAATGAT 
GTACACAGGG CCTTCAAGAG GGAATTGAAA ACGAAAGAAC CTGTAATCAT GAGTACTCTT 
GAGACTGTAC GAATATTTCT GACAGAGCAG CCTTTAGAAG GACTAGAGAA ACTCTACCAG 
GAGCCCAGAG AGCTGCCTCC TGAAGAGAGA GCCCAGAATG TCACACGGCT CCTACGAAAG 
CAAGCTGAGG AGGTCAACAC TCAGTGGGAA AAACTGAACG TGCACTCTGC AGACTGGCAG 
AGAAAAATAG ACGAGGCCCT CGAAAGACTC CAGGAGCTTC AGGAAGCAAC AGATGAGCTG 
GATCTCAAAC TACGTCAGGC AGAGGTGATC AAAGGATCCT GGCAGCCTGT GGGTGATCTC 
CTCATTGACT CTCTCCAAGA TCACCTCGAA AAAGTCAAGG CGCTTCGAGG AGAAATTACA 
CCTCTGAAAG AGAATGTCAG CTACGTCAAT GACCTTGCTC GCCAACTCAC TACGTTGGGC 
ATTCAGCTGT CACCATATAA CCTCAACACT CTGGAAGACC TGAACACCAG ATGGAAGCTT 
CTGCAGGTGG CCATTGAGGA CCGCATCAGG CAGCTGCATG AAGCGCACAG GGACTTTGGA 
CCAGCCTCCC AGCACTTCCT TTCCACTTCT GTCCAGGGTC CCTGGGAGAG AGCCATCTCA 
CCAAACAAAG TGCCCTACTA TATCAACCAC GAGACCCAAA CAACTTGCTG GGACCATCCC 
AAAATGACAG AGCTCTACCA GTCTTTAGCT GACCTGAATA ATGTCAGGTT CTCAGCTTAC 
AGGACTGCCA TGAAACTCCG AAGACTGCAG AAGGCCCTTT GCTTGGATCT CTTGAGCCTA 
TCGGCTGCAT GCGATGCCTT GGACCAGCAC AACCTCAAGC AAAATGACCA GCCCATGGAT 
ATCCTGCAGG TCATTAACTG TCTGACCACT ATTTATGATC GCCTAGAGCA AGAGCACAAC 
AATCTGGTCA ACGTCCCTCT CTGCGTGGAT ATGTGTCTCA ATTGGCTGCT GAATGTTTAT 
GACACGGGAC GAACGGGGAG GATCCGGGTC CTGTCTTTTA AAACTGGCAT CATTTCTCTG 
TGTAAAGCCC ATTTGGAAGA CAAGTACAGA TACCTCTTCA AGCAAGTGGC AAGTTCGACA 
GGATTTTGTG ACCAGCGCAG GCTGGGCCTC CTCCTGCATG ACTCTATCCA GATCCCAAGA 
CAGTTGGGTG AAGTCGCATC GTTCGGGGGC AGTAACATTG AGCCGAGTGT CAGGAGCTGC 
TTCCAGTTTG CTAATAATAA GCCTGAGATC GAAGCGGCCC TCTTCCTAGA CTGGATGCGC 
CTGGAGCCCC AGTCCATGGT GTGGCTGCCT GTCCTGCACC GAGTGGCTGC CGCGGAAACT 
GCCAAGCACC AGGCCAAGTG CAACATCTGC AAGGAGTGTC CCATCATCGG ATTCAGGTAC 
AGGAGTCTAA AGCACTTTAA TTATGACATC TGCCAAAGTT GCTTTTTTTC TGGTCGAGTT 
GCAAAAGGCC ATAAAATGCA CTATCCCATG GTGGAATACT GCACTCCGAC TACATCGGGA 
GAAGATGTCC GTGACTTTGC CAAGGTACTA AAAAACAAAT TTCGAACCAA AAGGTATTTT 
GCGAAGCATC CCCGAATGGG CTACCTGCCA GTGCAGACTG TCTTAGAGGG GGACAACATG 
GAAACTCCTG TCACTCTGAT CAACTTCTGG CCGGTAGATT CTGCGCCTGC CTCGTCCCCT 
CAGCTTTCAC ACGATGATAC TCATTCACGC ATTGAGCATT ATGCTAGCAG GCTAGCAGAA 
ATGGAAAACA GCAATGGATC TTATCTAAAT GATAGCATCT CTCCTAATGA GAGCATAGAT 
GATGAACATT TGTTAATCCA GCATTACTGC CAAAGTTTGA ACCAGGACTC CCCCCTGAGC 
CAGCCTCGTA GTCCTGCCCA GATCTTGATT TCCTTAGAGA GTGAGGAAAG AGGGGAGCTA 
GAGAGAATCC TAGCAGATCT TGAGGAAGAA AACAGAAATC TGCAAGCAGA GTATGATCGT 
CTAAAGCAGC AGCATGAACA CAAAGGCCTG TCCCCACTGC CATCCCCTCC TGAAATGATG 
CCTACTTCTC CCCAAAGTCC CCGGGATGCT GAGCTCATCG CTGAGGCCAA GCTGCTGCGT 
CAACACAAAG GCCGCCTGGA AGCCAGGATG CAAATCCTTG AAGACCATAA CAAACAACTG 
GAATCCCAGT TACACAGGCT CAGGCAGCTG CTGGAGCAAC CCCAGGCAGA GGCCAAGGTG 
AATGGTACAA CGGTGTCTTC TCCTTCTACC TCTCTTCAGA GGTCAGATAG CAGTCAGCCT 
ATGCTGCTCC GGGTGGTCGG CAGTCAGACT TCAGAATCCA TGGGCGAGGA AGACCTGCTC 
AGCCCTCCCC AGGACACAAG CACAGGGTTA GAGGAAGTGA TGGAGCAGCT CAACCACTCC 
TTCCCTAGTT CCAGAGGACA CAATGTAGGA AGCCTTTTCC ACTTGGCAGA TGATTTGGGC 
AGAGCGATGG AGTCCTTAGT TTCAGTCATG ACAGATGAAG AAGGGGCAGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013966817.1
CDS	247..11340
Translation	MLWWEEVEDCYEREDVQKKTFTKWINAQFSKFGKQHIENLFSDLQDGRRLLDLLEGLTGQKLPKEKGSTRVHALNNVNKALRVLQKNNVDLVNIGSTDIVDGNHKLTLGLIWNIILHWQVKNVMKNIMAGLQQTNSEKILLSWVRQSTRNYPQVNVINFTTSWSDGLALNALIHSHRPDLFDWNSVVCQQSATQRLEHAFNIAKYQLGIEKLLDPEDVATTYPDKKSILMYITSLFQVLPQQVSIEAIQEVEMLPRPSKVTREEHFQLHHQMHYSQQITVSLAQGYERAPSSPKPRFKSYAYTQAAYVTTSDPTRSPLPSQHLETPEDKSFGRSLTETEANLDSYQTALEEVLSWLLSAEDALQAQGEISNDVEEVKEQFHTHEGYMMDLTSHQGRVGNVLQLGSQLIGTGKLSEDEETEVQEQMNLLNSRWECLRVASMEKQSNLHKVLMDLQNQQLKELNDWLTKTEERTRKMEKEPLGPDIEDLKRQVQQHKVLQEDLEQEQVRVNSLTHMVVVVDESSGDHATAALEEQLKVLGDRWANICRWTEDRWVLLQDILLKWQRFTEEQCLFSAWLSEKEDAVNKIHTTGFKDQSEVLSNLQKLAVLKTDLEKKKQTMDKLCSLNQDLLSALKNTVVAHKMEAWLDNFAQRWDNLVQKLEKSSAQISQAVTTTQPSLTQTTVMETVTMVTTREHILVKHAQEELPPPPPQKKRQIIVDSEIRKRLDVDITELHSWITRSEAVLQSPEFAIYRKEGNFSDLKEKVNAIEREKAEKFRKLQDASRSAQALVEQMVNEGVNADSIKQASEQLNSRWIEFCQLLSERLNWLEYQNNIITFYNQLQQLEQMTTTAENWLKTQPTTTSEPTAIKSQLKICKDEINRLSALQPQIERLKIQSIALKEKGQGPMFLDADFVAFTNHFNQVFADVQAREKELQTIFDSLPPMRYQETMSTILTWIQQSETKLSIPQVTVTEYDIMEQRLGELQALQSSLQEQQNGLNYLSTTVKEMSKKAPLSDISRKYQSEFEEIEGRWKKLSSQLVEHCQKLEEQMAKLRKIQNHIKTLKKWITEVDVFLKEEWPALGDSEILKRQLKQCRLLVNDIQTIQPSLNSVNEGAQKMKNEAEPEFAGRLETELRELNTQWDYMCRQVYARKEALKGGLDKTVSLQKDLSEMHEWMTQAEEEYLERDFEYKTPDELQTAVEEMKRAKEEAQQKEAKVKLLTESVNSVIAQAPPAAQEALKKELDTLTTNYQWLCTRLNGKCKTLEEVWACWHELLSYLEKANKWLSEVEVKLKTTENISGGAEEIAEVLDSLENLMQHSEDNPNQIRILAQTLTDGGVMDELINEELETFNSRWRELHEEAVRRQKLLEQSIQSAQEIEKSLHLIQESLSSIDKQLAAYIADKVDAAQMPQEAQKIQSDLTSHEISLEEMKKHNQGKETAQRVLSQIDVAQKKLQDVSMKFRLFQKPANFEQRLQESKMILDEVKMHLPALETKSVEQEVVQSQLNHCVNLYKSLSEVKSEVEMVIKTGRQIVQKKQTENPKELDERVTALKLHYNELGAKVTERKQQLEKCLKLSRKMRKEMNALTEWLAATDMELTKRSAVEGMPSNLDSEVAWGKATQKEIEKQKVHLKSVTEVGEALKTVLGKKEMLVEDKLSLLNSNWIAVTSRAEEWLNLLLEYQKHMENFDQNVDYITNWIIQADALLDESEKKKPQQKEDILKRLKAEMNDIRPKVDSTRDQAANLMANRGDHCRKVVEPKISELNHRFAAISHRIKTGKASIPLKELEQFNSDIQKLLEPLEAEIQQGVNLKEEDFNKDMSEDNEGTVKELLQRGDNLQQRITDERKREEIKIKQQLLQTKHNALKDLRSQRRKKALEISHQWYQYKRQADDLLKCLDDIEKKLASLPEPRDERKIKEIDRELQKKKEELNAVRRQAEGLSEDGAAMAVEPTQIQLSKRWREIESKFAQFRRLNFAQIHTVHEESVVAMTEDMPLEISYVPSTYLTEITHVSQALSEVEELLNAPDLCAQDFEDLFKQEESLKNIKDSLQQISGRIDIIHNKKTAALHSATPAERAKLQEALSRLDFQWERVNNMYKDRQGRFDRSVEKWRRFHYDMKILNQWLTEAEQFLKKTQIPENWEHAKYKWYLKELQDGIGQRQSVVRVLNATGEEIIQQSSKTDASILQEKLGSLNLRWQEVCKQLAERKKRLEEQKNILSEFQRDVNEFVLWLEEADNVANIPLEPGNEQQLKEKLEQVKLLAEELPLRQGILKQLNETGGTVLVSAPLSPEEQDKLENKLKQTNLQWIKVSRNLPEKQEEIEAHVKDLGQLEEQLNHLLLWLSPIRNQLEIYNQPNQTGPFDIKEIEVAVQAKQPDVEGILSKGQHLYKEKPATQPAKRKLEDLSSDWKVVTQLLQELRAKQPGPAPGLTTVRAPPSQTVTLVTQPAVTKETAISKLEMPSSLLLEVPALADFNRAWTELTDWLSLLDRVIKSQRVMVGDLEDINEMIIKQKATLQDLEQRRPQLEELITAAQNLKNKTSNQEARTIITDRIERIQSQWDEVQEHLQNRRQQLNEMLKDSTQWLEAKEEAEQVLGQARAKLESWKEAPYTVDAIQKKITETKLAKDLRQWQINVDVANDLALKLLRDYSADDTRKVHMITENINASWASIHKRLSEREAALEETHRLLQQFPLDLEKFLAWLTEAETTANVLQDATHKERLLEDSKGVRELMKQWQDLQGEIEAHTDIYHNLDENGQKVLRSLEGSDDAALLQRRLDNMNFKWSELRKKSLNIRSHLEASSDQWKRLHLSLQELLVWLQLKDDELSRQAPIGGDFPAVQKQNDVHRAFKRELKTKEPVIMSTLETVRIFLTEQPLEGLEKLYQEPRELPPEERAQNVTRLLRKQAEEVNTQWEKLNVHSADWQRKIDEALERLQELQEATDELDLKLRQAEVIKGSWQPVGDLLIDSLQDHLEKVKALRGEITPLKENVSYVNDLARQLTTLGIQLSPYNLNTLEDLNTRWKLLQVAIEDRIRQLHEAHRDFGPASQHFLSTSVQGPWERAISPNKVPYYINHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQVINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPVTLINFWPVDSAPASSPQLSHDDTHSRIEHYASRLAEMENSNGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKQQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDTSTGLEEVMEQLNHSFPSSRGHNVGSLFHLADDLGRAMESLVSVMTDEEGAE

Target ORF information:

RefSeq Version	XM_014111342.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris dystrophin (DMD), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111342.2

ORF Insert Sequence:

ATGCTTTGGT GGGAAGAAGT AGAGGACTGT TATGAAAGAG AAGATGTTCA AAAGAAAACA 
TTCACAAAAT GGATAAATGC ACAGTTTTCT AAGTTTGGGA AGCAGCACAT AGAGAACCTC 
TTCAGTGACC TACAGGATGG GAGACGCCTC CTAGACCTTT TGGAAGGCCT GACAGGGCAA 
AAACTGCCAA AAGAAAAAGG ATCCACAAGA GTTCATGCCC TGAACAATGT CAACAAGGCA 
CTGCGCGTCT TGCAGAAAAA TAATGTTGAT TTAGTGAACA TTGGAAGTAC TGACATAGTA 
GATGGAAATC ACAAACTGAC TCTTGGTTTG ATTTGGAATA TAATCCTCCA CTGGCAGGTC 
AAAAATGTAA TGAAAAATAT CATGGCTGGA TTGCAACAAA CCAACAGTGA AAAGATTCTC 
CTGAGCTGGG TCCGACAATC AACTCGTAAT TATCCACAGG TTAATGTCAT TAACTTCACC 
ACCAGCTGGT CTGATGGCCT GGCTTTGAAC GCTCTCATCC ACAGTCATAG GCCAGACCTG 
TTTGATTGGA ATAGTGTGGT TTGCCAGCAG TCAGCCACAC AACGCCTGGA ACATGCATTC 
AACATTGCCA AATATCAATT AGGCATAGAG AAACTGCTTG ATCCTGAAGA TGTTGCCACC 
ACTTATCCAG ATAAGAAGTC CATCTTAATG TATATCACAT CACTCTTCCA AGTTTTGCCT 
CAACAAGTGA GCATTGAAGC CATCCAGGAA GTGGAAATGT TGCCAAGGCC ATCAAAAGTT 
ACTAGAGAAG AACATTTTCA ATTACATCAT CAAATGCACT ATTCTCAACA GATCACAGTC 
AGTCTAGCAC AGGGCTATGA ACGAGCCCCT TCCTCTCCTA AGCCTCGGTT CAAGAGCTAT 
GCCTACACAC AGGCTGCTTA TGTCACCACT TCTGACCCCA CACGGAGCCC ACTTCCTTCA 
CAGCATTTGG AAACTCCTGA AGACAAGTCA TTTGGCCGGT CATTGACAGA GACCGAAGCA 
AACCTGGACA GTTATCAAAC AGCTTTGGAA GAAGTACTCT CGTGGCTTCT TTCAGCTGAG 
GATGCACTGC AAGCCCAAGG AGAGATTTCT AATGATGTCG AAGAAGTGAA AGAACAATTT 
CATACTCATG AGGGATATAT GATGGACTTG ACATCCCATC AGGGACGGGT CGGTAATGTT 
CTCCAACTGG GAAGTCAACT GATTGGAACA GGGAAATTAT CAGAAGATGA AGAAACCGAA 
GTGCAGGAAC AAATGAATCT CCTCAATTCA AGATGGGAAT GCCTCAGGGT AGCTAGCATG 
GAAAAACAAA GCAATTTACA TAAAGTTCTA ATGGATCTCC AGAATCAGCA ACTGAAAGAG 
TTAAATGACT GGCTAACCAA AACAGAAGAG AGAACAAGGA AAATGGAGAA GGAGCCCCTT 
GGACCTGATA TTGAAGACCT AAAACGCCAA GTACAACAAC ATAAGGTGCT TCAAGAAGAC 
TTAGAACAGG AACAAGTCAG GGTCAATTCC CTCACTCATA TGGTGGTGGT AGTCGATGAA 
TCTAGTGGAG ACCATGCAAC TGCTGCTTTG GAAGAACAAC TTAAGGTACT GGGAGATCGA 
TGGGCAAACA TCTGTAGGTG GACAGAAGAT CGCTGGGTTC TTTTACAAGA CATCCTCCTA 
AAATGGCAGC GTTTTACTGA AGAACAGTGC CTTTTCAGTG CATGGCTTTC GGAGAAGGAA 
GATGCAGTGA ACAAGATTCA CACAACTGGC TTTAAGGATC AAAGTGAAGT GTTATCAAAT 
CTTCAGAAAC TGGCTGTCTT AAAAACAGAT CTGGAAAAGA AGAAGCAAAC CATGGACAAA 
CTCTGCTCAC TCAACCAAGA CCTTCTTTCA GCGCTGAAAA ACACAGTGGT AGCCCACAAG 
ATGGAAGCAT GGCTGGACAA CTTTGCCCAG CGCTGGGATA ATTTAGTCCA GAAACTTGAA 
AAAAGTTCAG CACAGATTTC ACAGGCTGTC ACCACCACTC AGCCATCACT AACACAGACA 
ACTGTAATGG AAACAGTAAC TATGGTGACC ACGAGGGAAC ACATCTTGGT AAAGCATGCC 
CAAGAGGAAC TGCCACCACC ACCCCCTCAG AAGAAGAGGC AGATTATCGT GGATTCTGAA 
ATTAGGAAAA GGTTGGATGT CGATATAACT GAACTTCACA GTTGGATTAC TCGTTCAGAA 
GCTGTGTTGC AGAGTCCTGA ATTTGCAATC TATCGGAAGG AAGGCAACTT CTCAGACCTT 
AAAGAAAAAG TCAATGCCAT AGAGCGAGAA AAAGCCGAGA AGTTCAGAAA ACTGCAAGAT 
GCCAGCAGAT CAGCTCAGGC CCTGGTGGAA CAGATGGTGA ATGAGGGTGT TAATGCTGAC 
AGCATCAAAC AAGCCTCCGA ACAACTGAAC AGCCGGTGGA TAGAGTTCTG CCAATTGCTA 
AGCGAGAGAC TTAACTGGCT GGAGTATCAG AACAACATCA TCACTTTCTA TAATCAGCTA 
CAACAATTGG AGCAGATGAC AACTACTGCT GAAAACTGGT TGAAAACCCA GCCTACCACC 
ACATCAGAGC CAACAGCAAT TAAAAGCCAG TTAAAAATTT GTAAGGATGA AATCAACCGA 
CTGTCAGCTC TTCAGCCTCA AATCGAGCGA TTAAAAATTC AAAGCATAGC CCTGAAAGAG 
AAAGGACAAG GGCCAATGTT CCTGGATGCA GACTTTGTGG CCTTTACAAA TCATTTTAAC 
CAAGTCTTTG CTGATGTGCA GGCAAGAGAA AAAGAGCTAC AAACAATTTT TGACAGTTTG 
CCACCCATGC GCTATCAGGA GACTATGAGT ACCATCCTGA CATGGATCCA GCAGTCAGAA 
ACCAAACTCT CTATACCTCA GGTTACTGTC ACTGAATATG ACATCATGGA ACAGAGACTC 
GGAGAGCTAC AGGCTTTACA AAGCTCTCTG CAAGAGCAAC AAAATGGCCT AAACTATCTC 
AGCACCACTG TGAAAGAGAT GTCAAAGAAA GCACCACTGT CTGATATTAG TCGGAAATAT 
CAATCAGAAT TTGAAGAGAT TGAGGGACGT TGGAAGAAGC TGTCTTCCCA GCTGGTTGAA 
CATTGTCAAA AGTTGGAGGA GCAAATGGCT AAACTTCGAA AAATTCAGAA TCACATAAAA 
ACTCTGAAGA AATGGATCAC TGAAGTCGAT GTTTTCCTGA AGGAGGAATG GCCTGCCCTT 
GGGGATTCAG AAATTCTGAA AAGACAGCTG AAACAGTGCA GGCTTTTAGT CAATGACATT 
CAGACCATCC AGCCTAGTCT CAACAGTGTC AATGAAGGGG CTCAGAAGAT GAAGAATGAA 
GCAGAACCAG AGTTTGCTGG CAGACTTGAG ACAGAGCTCC GAGAACTTAA CACCCAGTGG 
GATTACATGT GCCGCCAGGT CTATGCCAGG AAGGAAGCCT TAAAAGGAGG TTTGGATAAA 
ACTGTAAGTC TTCAGAAAGA TCTGTCAGAG ATGCATGAGT GGATGACACA AGCTGAAGAA 
GAATACCTAG AGAGAGATTT CGAATACAAG ACCCCTGATG AATTACAGAC AGCAGTTGAA 
GAGATGAAGA GAGCTAAAGA AGAGGCCCAG CAAAAAGAAG CAAAAGTGAA ACTCCTAACC 
GAGTCCGTCA ATAGTGTCAT AGCTCAGGCT CCACCTGCAG CACAAGAGGC CTTAAAAAAG 
GAACTTGACA CTCTCACCAC CAACTACCAG TGGCTCTGCA CCAGGCTCAA TGGCAAATGC 
AAGACCTTGG AAGAAGTTTG GGCGTGCTGG CATGAGTTAT TGTCCTACTT GGAGAAGGCA 
AACAAGTGGC TAAGTGAAGT AGAAGTCAAG CTTAAAACCA CTGAAAATAT TTCTGGGGGA 
GCTGAGGAAA TCGCCGAGGT GCTTGATTCG CTTGAAAATT TGATGCAACA TTCAGAGGAT 
AACCCGAATC AGATTCGCAT ATTGGCACAG ACCTTGACAG ATGGTGGAGT CATGGATGAA 
CTGATCAATG AGGAGCTTGA GACATTTAAT TCTCGTTGGA GAGAACTCCA TGAAGAGGCT 
GTGAGGAGGC AAAAGTTGCT TGAGCAGAGT ATCCAGTCGG CCCAGGAGAT AGAAAAATCC 
TTGCACTTAA TTCAGGAGTC CCTCTCTTCC ATTGACAAGC AGTTGGCAGC TTATATTGCT 
GACAAAGTGG ATGCAGCTCA GATGCCTCAG GAAGCCCAGA AAATCCAATC AGATTTGACA 
AGTCATGAGA TCAGTTTAGA AGAAATGAAG AAACATAACC AGGGAAAGGA GACTGCCCAA 
AGGGTACTAT CCCAAATTGA TGTGGCACAG AAAAAATTGC AGGATGTTTC CATGAAGTTT 
CGATTATTCC AGAAGCCAGC CAATTTTGAG CAGCGCCTAC AAGAAAGTAA AATGATTTTA 
GATGAAGTGA AGATGCATTT ACCTGCGTTG GAAACAAAGA GTGTGGAACA GGAAGTAGTA 
CAGTCACAGT TAAATCATTG TGTGAACTTG TATAAAAGTC TGAGTGAAGT GAAGTCTGAA 
GTGGAAATGG TAATAAAAAC TGGACGTCAG ATTGTACAGA AGAAGCAGAC GGAAAACCCG 
AAAGAGCTTG ATGAAAGAGT TACAGCTTTG AAATTGCATT ATAATGAGCT GGGAGCAAAG 
GTGACAGAAA GAAAGCAACA GTTGGAAAAA TGCTTGAAAT TGTCCCGTAA GATGCGAAAG 
GAAATGAATG CCCTGACAGA ATGGCTGGCA GCGACAGATA TGGAACTGAC GAAGAGATCG 
GCAGTTGAAG GAATGCCTAG TAATTTGGAT TCTGAAGTTG CCTGGGGAAA GGCTACTCAG 
AAAGAGATTG AGAAACAGAA GGTTCACCTA AAGAGTGTCA CAGAGGTAGG AGAGGCCTTG 
AAAACGGTTT TGGGCAAGAA GGAAATGTTG GTGGAAGATA AACTGAGTCT TCTGAATAGT 
AACTGGATAG CCGTCACTTC CCGAGCAGAA GAGTGGTTAA ACCTTTTATT GGAATACCAG 
AAACACATGG AAAACTTTGA CCAGAATGTG GATTACATCA CAAACTGGAT CATTCAGGCT 
GATGCACTTT TGGATGAATC TGAGAAAAAG AAACCTCAGC AAAAAGAAGA CATACTTAAG 
CGTTTAAAGG CTGAAATGAA TGACATACGT CCAAAGGTGG ATTCTACACG TGACCAAGCA 
GCAAACCTGA TGGCAAACCG CGGCGACCAC TGCAGGAAAG TAGTAGAGCC CAAAATCTCA 
GAGCTCAACC ATCGATTTGC AGCCATTTCT CACAGAATTA AGACTGGAAA GGCCTCCATT 
CCTTTGAAGG AATTGGAGCA GTTTAACTCA GATATACAAA AATTGCTTGA ACCACTGGAG 
GCTGAAATTC AGCAGGGGGT GAATCTGAAA GAGGAAGACT TCAATAAAGA TATGAGTGAA 
GACAATGAGG GTACTGTAAA AGAATTGTTG CAAAGAGGAG ACAACTTACA ACAAAGAATC 
ACAGATGAGA GAAAGCGAGA GGAAATAAAG ATAAAACAAC AGCTGTTACA GACAAAACAT 
AATGCTCTCA AGGATTTGAG GTCTCAAAGA AGAAAAAAGG CTCTAGAAAT TTCTCACCAG 
TGGTATCAGT ACAAGAGGCA GGCTGATGAT CTCCTGAAAT GCTTGGATGA CATTGAAAAA 
AAATTAGCCA GCCTACCTGA ACCCAGAGAT GAAAGGAAAA TAAAGGAAAT TGATCGTGAA 
TTGCAGAAGA AGAAAGAGGA GCTGAATGCA GTGCGTAGGC AAGCTGAGGG CTTGTCTGAG 
GATGGGGCCG CAATGGCAGT GGAGCCAACT CAGATCCAGC TCAGCAAGCG CTGGCGGGAA 
ATTGAGAGCA AATTTGCTCA GTTTCGAAGA CTCAACTTTG CACAAATTCA CACTGTCCAT 
GAAGAGTCAG TGGTGGCGAT GACTGAAGAC ATGCCTTTGG AAATTTCTTA TGTGCCTTCT 
ACTTACCTGA CTGAGATCAC TCATGTCTCA CAAGCCCTAT CAGAAGTGGA AGAGCTTCTT 
AATGCTCCCG ACCTTTGTGC TCAAGATTTT GAAGATCTCT TTAAACAAGA GGAATCCTTG 
AAGAACATAA AAGACAGCCT GCAACAAATC TCAGGTCGGA TTGACATCAT TCACAATAAA 
AAGACAGCAG CATTGCACAG TGCCACTCCT GCAGAAAGGG CAAAGCTCCA GGAAGCTCTC 
TCACGGCTTG ATTTCCAATG GGAAAGAGTT AACAATATGT ACAAGGACCG ACAAGGGAGA 
TTTGACAGAT CTGTGGAAAA ATGGCGGCGG TTTCATTATG ATATGAAGAT ACTTAATCAA 
TGGCTAACAG AAGCTGAACA GTTTCTCAAA AAGACACAAA TTCCTGAGAA TTGGGAACAT 
GCCAAATACA AATGGTATCT TAAGGAACTC CAGGATGGCA TTGGACAGCG GCAAAGTGTT 
GTCAGGGTAT TGAATGCAAC TGGGGAAGAA ATAATTCAAC AGTCCTCAAA AACAGATGCC 
AGTATTCTCC AAGAAAAACT GGGAAGCCTG AATCTGCGGT GGCAGGAGGT CTGCAAACAG 
CTGGCAGAAA GAAAAAAGAG GCTAGAGGAA CAGAAGAATA TCTTGTCAGA ATTTCAAAGA 
GATGTAAATG AATTTGTTTT ATGGTTGGAA GAAGCGGATA ACGTTGCTAA TATTCCACTT 
GAACCTGGAA ATGAGCAGCA GCTAAAAGAA AAACTTGAAC AAGTCAAGTT ACTGGCAGAA 
GAGTTGCCCC TGCGCCAGGG AATTCTAAAA CAATTAAATG AAACTGGAGG AACAGTGCTT 
GTAAGTGCTC CCCTAAGCCC AGAAGAGCAA GATAAACTTG AAAATAAGCT CAAGCAGACA 
AATCTTCAGT GGATAAAGGT TTCTAGAAAT CTGCCTGAGA AGCAAGAAGA AATTGAGGCA 
CACGTAAAAG ACCTTGGACA GCTGGAAGAG CAGTTAAATC ATCTGCTTCT ATGGCTGTCT 
CCTATTAGGA ATCAGTTGGA AATTTACAAT CAGCCAAATC AAACAGGACC ATTTGACATC 
AAGGAAATTG AAGTAGCAGT TCAAGCTAAA CAGCCGGATG TGGAAGGGAT TTTGTCTAAA 
GGGCAGCATT TGTACAAGGA AAAACCAGCC ACTCAGCCAG CGAAGAGAAA GCTGGAAGAT 
CTCAGCTCTG ATTGGAAGGT GGTAACTCAG TTGCTTCAAG AGCTGCGGGC AAAGCAGCCT 
GGCCCAGCTC CTGGACTGAC CACTGTCAGA GCCCCTCCCA GTCAGACTGT TACTCTGGTG 
ACACAACCCG CGGTTACCAA GGAAACTGCC ATCTCCAAAC TAGAAATGCC ATCTTCATTG 
CTGTTGGAGG TACCTGCACT GGCAGATTTC AACCGAGCTT GGACAGAACT TACCGACTGG 
CTGTCTCTGC TTGATCGAGT TATAAAATCA CAGAGGGTGA TGGTGGGTGA TCTTGAAGAC 
ATTAACGAGA TGATCATCAA GCAGAAGGCA ACGCTGCAGG ATTTGGAACA GAGGCGCCCC 
CAGTTGGAAG AACTCATTAC CGCTGCCCAG AATTTGAAAA ACAAGACCAG CAATCAAGAG 
GCTAGAACAA TCATTACTGA TCGAATTGAA AGAATTCAGA GTCAGTGGGA TGAAGTACAG 
GAACATCTTC AGAACCGGAG GCAACAGTTG AATGAAATGT TAAAGGATTC CACACAATGG 
CTGGAAGCTA AAGAGGAGGC TGAGCAGGTG TTGGGGCAGG CCAGAGCCAA GCTTGAGTCA 
TGGAAGGAGG CTCCCTACAC AGTAGATGCA ATCCAAAAGA AAATCACAGA AACCAAGTTG 
GCCAAAGACC TCCGCCAGTG GCAGATAAAT GTAGATGTTG CAAATGATTT GGCACTGAAA 
CTTCTCCGAG ATTATTCTGC AGATGATACC AGAAAAGTAC ACATGATAAC AGAGAACATC 
AATGCCTCTT GGGCAAGCAT CCATAAAAGA TTGAGTGAGC GAGAGGCTGC TCTGGAAGAA 
ACCCACAGAT TACTGCAACA GTTCCCCTTG GACCTGGAGA AGTTCCTTGC CTGGCTTACA 
GAAGCCGAAA CAACTGCCAA CGTCCTGCAG GATGCCACCC ATAAGGAAAG GCTTCTAGAA 
GATTCCAAGG GAGTAAGAGA GCTGATGAAA CAATGGCAAG ATCTCCAAGG AGAAATCGAA 
GCTCACACAG ATATCTATCA CAACCTGGAC GAAAATGGCC AAAAAGTCCT GAGATCCCTG 
GAAGGTTCTG ACGATGCAGC CTTGTTGCAA AGACGTTTGG ATAACATGAA CTTCAAGTGG 
AGCGAACTTC GGAAAAAGTC TCTCAACATT AGGTCTCACT TGGAAGCCAG TTCTGACCAG 
TGGAAGCGTC TGCACCTTTC TCTTCAGGAA CTTCTGGTAT GGCTCCAGCT GAAAGATGAT 
GAGTTAAGCC GGCAGGCACC CATTGGAGGA GACTTTCCAG CGGTGCAGAA GCAGAATGAT 
GTACACAGGG CCTTCAAGAG GGAATTGAAA ACGAAAGAAC CTGTAATCAT GAGTACTCTT 
GAGACTGTAC GAATATTTCT GACAGAGCAG CCTTTAGAAG GACTAGAGAA ACTCTACCAG 
GAGCCCAGAG AGCTGCCTCC TGAAGAGAGA GCCCAGAATG TCACACGGCT CCTACGAAAG 
CAAGCTGAGG AGGTCAACAC TCAGTGGGAA AAACTGAACG TGCACTCTGC AGACTGGCAG 
AGAAAAATAG ACGAGGCCCT CGAAAGACTC CAGGAGCTTC AGGAAGCAAC AGATGAGCTG 
GATCTCAAAC TACGTCAGGC AGAGGTGATC AAAGGATCCT GGCAGCCTGT GGGTGATCTC 
CTCATTGACT CTCTCCAAGA TCACCTCGAA AAAGTCAAGG CGCTTCGAGG AGAAATTACA 
CCTCTGAAAG AGAATGTCAG CTACGTCAAT GACCTTGCTC GCCAACTCAC TACGTTGGGC 
ATTCAGCTGT CACCATATAA CCTCAACACT CTGGAAGACC TGAACACCAG ATGGAAGCTT 
CTGCAGGTGG CCATTGAGGA CCGCATCAGG CAGCTGCATG AAGCGCACAG GGACTTTGGA 
CCAGCCTCCC AGCACTTCCT TTCCACTTCT GTCCAGGGTC CCTGGGAGAG AGCCATCTCA 
CCAAACAAAG TGCCCTACTA TATCAACCAC GAGACCCAAA CAACTTGCTG GGACCATCCC 
AAAATGACAG AGCTCTACCA GTCTTTAGCT GACCTGAATA ATGTCAGGTT CTCAGCTTAC 
AGGACTGCCA TGAAACTCCG AAGACTGCAG AAGGCCCTTT GCTTGGATCT CTTGAGCCTA 
TCGGCTGCAT GCGATGCCTT GGACCAGCAC AACCTCAAGC AAAATGACCA GCCCATGGAT 
ATCCTGCAGG TCATTAACTG TCTGACCACT ATTTATGATC GCCTAGAGCA AGAGCACAAC 
AATCTGGTCA ACGTCCCTCT CTGCGTGGAT ATGTGTCTCA ATTGGCTGCT GAATGTTTAT 
GACACGGGAC GAACGGGGAG GATCCGGGTC CTGTCTTTTA AAACTGGCAT CATTTCTCTG 
TGTAAAGCCC ATTTGGAAGA CAAGTACAGA TACCTCTTCA AGCAAGTGGC AAGTTCGACA 
GGATTTTGTG ACCAGCGCAG GCTGGGCCTC CTCCTGCATG ACTCTATCCA GATCCCAAGA 
CAGTTGGGTG AAGTCGCATC GTTCGGGGGC AGTAACATTG AGCCGAGTGT CAGGAGCTGC 
TTCCAGTTTG CTAATAATAA GCCTGAGATC GAAGCGGCCC TCTTCCTAGA CTGGATGCGC 
CTGGAGCCCC AGTCCATGGT GTGGCTGCCT GTCCTGCACC GAGTGGCTGC CGCGGAAACT 
GCCAAGCACC AGGCCAAGTG CAACATCTGC AAGGAGTGTC CCATCATCGG ATTCAGGTAC 
AGGAGTCTAA AGCACTTTAA TTATGACATC TGCCAAAGTT GCTTTTTTTC TGGTCGAGTT 
GCAAAAGGCC ATAAAATGCA CTATCCCATG GTGGAATACT GCACTCCGAC TACATCGGGA 
GAAGATGTCC GTGACTTTGC CAAGGTACTA AAAAACAAAT TTCGAACCAA AAGGTATTTT 
GCGAAGCATC CCCGAATGGG CTACCTGCCA GTGCAGACTG TCTTAGAGGG GGACAACATG 
GAAACTCCTG TCACTCTGAT CAACTTCTGG CCGGTAGATT CTGCGCCTGC CTCGTCCCCT 
CAGCTTTCAC ACGATGATAC TCATTCACGC ATTGAGCATT ATGCTAGCAG GCTAGCAGAA 
ATGGAAAACA GCAATGGATC TTATCTAAAT GATAGCATCT CTCCTAATGA GAGCATAGAT 
GATGAACATT TGTTAATCCA GCATTACTGC CAAAGTTTGA ACCAGGACTC CCCCCTGAGC 
CAGCCTCGTA GTCCTGCCCA GATCTTGATT TCCTTAGAGA GTGAGGAAAG AGGGGAGCTA 
GAGAGAATCC TAGCAGATCT TGAGGAAGAA AACAGAAATC TGCAAGCAGA GTATGATCGT 
CTAAAGCAGC AGCATGAACA CAAAGGCCTG TCCCCACTGC CATCCCCTCC TGAAATGATG 
CCTACTTCTC CCCAAAGTCC CCGGGATGCT GAGCTCATCG CTGAGGCCAA GCTGCTGCGT 
CAACACAAAG GCCGCCTGGA AGCCAGGATG CAAATCCTTG AAGACCATAA CAAACAACTG 
GAATCCCAGT TACACAGGCT CAGGCAGCTG CTGGAGCAAC CCCAGGCAGA GGCCAAGGTG 
AATGGTACAA CGGTGTCTTC TCCTTCTACC TCTCTTCAGA GGTCAGATAG CAGTCAGCCT 
ATGCTGCTCC GGGTGGTCGG CAGTCAGACT TCAGAATCCA TGGGCGAGGA AGACCTGCTC 
AGCCCTCCCC AGGACACAAG CACAGGGTTA GAGGAAGTGA TGGAGCAGCT CAACCACTCC 
TTCCCTAGTT CCAGAGGACA CAATGTAGGA AGCCTTTTCC ACTTGGCAGA TGATTTGGGC 
AGAGCGATGG AGTCCTTAGT TTCAGTCATG ACAGATGAAG AAGGGGCAGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMD ( XM_014111343.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013966818.1 Canis lupus familiaris dystrophin (DMD), transcript variant X3, mRNA.

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CloneID	OCe45414
Clone ID Related Accession (Same CDS sequence)	XM_014111343.2 , XM_014111343.1
Accession Version	XM_014111343.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	dystrophin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014111343.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGAGG TCTCATCTGA TGAAAGAGAA GATGTTCAAA AGAAAACATT CACAAAATGG 
ATAAATGCAC AGTTTTCTAA GTTTGGGAAG CAGCACATAG AGAACCTCTT CAGTGACCTA 
CAGGATGGGA GACGCCTCCT AGACCTTTTG GAAGGCCTGA CAGGGCAAAA ACTGCCAAAA 
GAAAAAGGAT CCACAAGAGT TCATGCCCTG AACAATGTCA ACAAGGCACT GCGCGTCTTG 
CAGAAAAATA ATGTTGATTT AGTGAACATT GGAAGTACTG ACATAGTAGA TGGAAATCAC 
AAACTGACTC TTGGTTTGAT TTGGAATATA ATCCTCCACT GGCAGGTCAA AAATGTAATG 
AAAAATATCA TGGCTGGATT GCAACAAACC AACAGTGAAA AGATTCTCCT GAGCTGGGTC 
CGACAATCAA CTCGTAATTA TCCACAGGTT AATGTCATTA ACTTCACCAC CAGCTGGTCT 
GATGGCCTGG CTTTGAACGC TCTCATCCAC AGTCATAGGC CAGACCTGTT TGATTGGAAT 
AGTGTGGTTT GCCAGCAGTC AGCCACACAA CGCCTGGAAC ATGCATTCAA CATTGCCAAA 
TATCAATTAG GCATAGAGAA ACTGCTTGAT CCTGAAGATG TTGCCACCAC TTATCCAGAT 
AAGAAGTCCA TCTTAATGTA TATCACATCA CTCTTCCAAG TTTTGCCTCA ACAAGTGAGC 
ATTGAAGCCA TCCAGGAAGT GGAAATGTTG CCAAGGCCAT CAAAAGTTAC TAGAGAAGAA 
CATTTTCAAT TACATCATCA AATGCACTAT TCTCAACAGA TCACAGTCAG TCTAGCACAG 
GGCTATGAAC GAGCCCCTTC CTCTCCTAAG CCTCGGTTCA AGAGCTATGC CTACACACAG 
GCTGCTTATG TCACCACTTC TGACCCCACA CGGAGCCCAC TTCCTTCACA GCATTTGGAA 
ACTCCTGAAG ACAAGTCATT TGGCCGGTCA TTGACAGAGA CCGAAGCAAA CCTGGACAGT 
TATCAAACAG CTTTGGAAGA AGTACTCTCG TGGCTTCTTT CAGCTGAGGA TGCACTGCAA 
GCCCAAGGAG AGATTTCTAA TGATGTCGAA GAAGTGAAAG AACAATTTCA TACTCATGAG 
GGATATATGA TGGACTTGAC ATCCCATCAG GGACGGGTCG GTAATGTTCT CCAACTGGGA 
AGTCAACTGA TTGGAACAGG GAAATTATCA GAAGATGAAG AAACCGAAGT GCAGGAACAA 
ATGAATCTCC TCAATTCAAG ATGGGAATGC CTCAGGGTAG CTAGCATGGA AAAACAAAGC 
AATTTACATA AAGTTCTAAT GGATCTCCAG AATCAGCAAC TGAAAGAGTT AAATGACTGG 
CTAACCAAAA CAGAAGAGAG AACAAGGAAA ATGGAGAAGG AGCCCCTTGG ACCTGATATT 
GAAGACCTAA AACGCCAAGT ACAACAACAT AAGGTGCTTC AAGAAGACTT AGAACAGGAA 
CAAGTCAGGG TCAATTCCCT CACTCATATG GTGGTGGTAG TCGATGAATC TAGTGGAGAC 
CATGCAACTG CTGCTTTGGA AGAACAACTT AAGGTACTGG GAGATCGATG GGCAAACATC 
TGTAGGTGGA CAGAAGATCG CTGGGTTCTT TTACAAGACA TCCTCCTAAA ATGGCAGCGT 
TTTACTGAAG AACAGTGCCT TTTCAGTGCA TGGCTTTCGG AGAAGGAAGA TGCAGTGAAC 
AAGATTCACA CAACTGGCTT TAAGGATCAA AGTGAAGTGT TATCAAATCT TCAGAAACTG 
GCTGTCTTAA AAACAGATCT GGAAAAGAAG AAGCAAACCA TGGACAAACT CTGCTCACTC 
AACCAAGACC TTCTTTCAGC GCTGAAAAAC ACAGTGGTAG CCCACAAGAT GGAAGCATGG 
CTGGACAACT TTGCCCAGCG CTGGGATAAT TTAGTCCAGA AACTTGAAAA AAGTTCAGCA 
CAGATTTCAC AGGCTGTCAC CACCACTCAG CCATCACTAA CACAGACAAC TGTAATGGAA 
ACAGTAACTA TGGTGACCAC GAGGGAACAC ATCTTGGTAA AGCATGCCCA AGAGGAACTG 
CCACCACCAC CCCCTCAGAA GAAGAGGCAG ATTATCGTGG ATTCTGAAAT TAGGAAAAGG 
TTGGATGTCG ATATAACTGA ACTTCACAGT TGGATTACTC GTTCAGAAGC TGTGTTGCAG 
AGTCCTGAAT TTGCAATCTA TCGGAAGGAA GGCAACTTCT CAGACCTTAA AGAAAAAGTC 
AATGCCATAG AGCGAGAAAA AGCCGAGAAG TTCAGAAAAC TGCAAGATGC CAGCAGATCA 
GCTCAGGCCC TGGTGGAACA GATGGTGAAT GAGGGTGTTA ATGCTGACAG CATCAAACAA 
GCCTCCGAAC AACTGAACAG CCGGTGGATA GAGTTCTGCC AATTGCTAAG CGAGAGACTT 
AACTGGCTGG AGTATCAGAA CAACATCATC ACTTTCTATA ATCAGCTACA ACAATTGGAG 
CAGATGACAA CTACTGCTGA AAACTGGTTG AAAACCCAGC CTACCACCAC ATCAGAGCCA 
ACAGCAATTA AAAGCCAGTT AAAAATTTGT AAGGATGAAA TCAACCGACT GTCAGCTCTT 
CAGCCTCAAA TCGAGCGATT AAAAATTCAA AGCATAGCCC TGAAAGAGAA AGGACAAGGG 
CCAATGTTCC TGGATGCAGA CTTTGTGGCC TTTACAAATC ATTTTAACCA AGTCTTTGCT 
GATGTGCAGG CAAGAGAAAA AGAGCTACAA ACAATTTTTG ACAGTTTGCC ACCCATGCGC 
TATCAGGAGA CTATGAGTAC CATCCTGACA TGGATCCAGC AGTCAGAAAC CAAACTCTCT 
ATACCTCAGG TTACTGTCAC TGAATATGAC ATCATGGAAC AGAGACTCGG AGAGCTACAG 
GCTTTACAAA GCTCTCTGCA AGAGCAACAA AATGGCCTAA ACTATCTCAG CACCACTGTG 
AAAGAGATGT CAAAGAAAGC ACCACTGTCT GATATTAGTC GGAAATATCA ATCAGAATTT 
GAAGAGATTG AGGGACGTTG GAAGAAGCTG TCTTCCCAGC TGGTTGAACA TTGTCAAAAG 
TTGGAGGAGC AAATGGCTAA ACTTCGAAAA ATTCAGAATC ACATAAAAAC TCTGAAGAAA 
TGGATCACTG AAGTCGATGT TTTCCTGAAG GAGGAATGGC CTGCCCTTGG GGATTCAGAA 
ATTCTGAAAA GACAGCTGAA ACAGTGCAGG CTTTTAGTCA ATGACATTCA GACCATCCAG 
CCTAGTCTCA ACAGTGTCAA TGAAGGGGCT CAGAAGATGA AGAATGAAGC AGAACCAGAG 
TTTGCTGGCA GACTTGAGAC AGAGCTCCGA GAACTTAACA CCCAGTGGGA TTACATGTGC 
CGCCAGGTCT ATGCCAGGAA GGAAGCCTTA AAAGGAGGTT TGGATAAAAC TGTAAGTCTT 
CAGAAAGATC TGTCAGAGAT GCATGAGTGG ATGACACAAG CTGAAGAAGA ATACCTAGAG 
AGAGATTTCG AATACAAGAC CCCTGATGAA TTACAGACAG CAGTTGAAGA GATGAAGAGA 
GCTAAAGAAG AGGCCCAGCA AAAAGAAGCA AAAGTGAAAC TCCTAACCGA GTCCGTCAAT 
AGTGTCATAG CTCAGGCTCC ACCTGCAGCA CAAGAGGCCT TAAAAAAGGA ACTTGACACT 
CTCACCACCA ACTACCAGTG GCTCTGCACC AGGCTCAATG GCAAATGCAA GACCTTGGAA 
GAAGTTTGGG CGTGCTGGCA TGAGTTATTG TCCTACTTGG AGAAGGCAAA CAAGTGGCTA 
AGTGAAGTAG AAGTCAAGCT TAAAACCACT GAAAATATTT CTGGGGGAGC TGAGGAAATC 
GCCGAGGTGC TTGATTCGCT TGAAAATTTG ATGCAACATT CAGAGGATAA CCCGAATCAG 
ATTCGCATAT TGGCACAGAC CTTGACAGAT GGTGGAGTCA TGGATGAACT GATCAATGAG 
GAGCTTGAGA CATTTAATTC TCGTTGGAGA GAACTCCATG AAGAGGCTGT GAGGAGGCAA 
AAGTTGCTTG AGCAGAGTAT CCAGTCGGCC CAGGAGATAG AAAAATCCTT GCACTTAATT 
CAGGAGTCCC TCTCTTCCAT TGACAAGCAG TTGGCAGCTT ATATTGCTGA CAAAGTGGAT 
GCAGCTCAGA TGCCTCAGGA AGCCCAGAAA ATCCAATCAG ATTTGACAAG TCATGAGATC 
AGTTTAGAAG AAATGAAGAA ACATAACCAG GGAAAGGAGA CTGCCCAAAG GGTACTATCC 
CAAATTGATG TGGCACAGAA AAAATTGCAG GATGTTTCCA TGAAGTTTCG ATTATTCCAG 
AAGCCAGCCA ATTTTGAGCA GCGCCTACAA GAAAGTAAAA TGATTTTAGA TGAAGTGAAG 
ATGCATTTAC CTGCGTTGGA AACAAAGAGT GTGGAACAGG AAGTAGTACA GTCACAGTTA 
AATCATTGTG TGAACTTGTA TAAAAGTCTG AGTGAAGTGA AGTCTGAAGT GGAAATGGTA 
ATAAAAACTG GACGTCAGAT TGTACAGAAG AAGCAGACGG AAAACCCGAA AGAGCTTGAT 
GAAAGAGTTA CAGCTTTGAA ATTGCATTAT AATGAGCTGG GAGCAAAGGT GACAGAAAGA 
AAGCAACAGT TGGAAAAATG CTTGAAATTG TCCCGTAAGA TGCGAAAGGA AATGAATGCC 
CTGACAGAAT GGCTGGCAGC GACAGATATG GAACTGACGA AGAGATCGGC AGTTGAAGGA 
ATGCCTAGTA ATTTGGATTC TGAAGTTGCC TGGGGAAAGG CTACTCAGAA AGAGATTGAG 
AAACAGAAGG TTCACCTAAA GAGTGTCACA GAGGTAGGAG AGGCCTTGAA AACGGTTTTG 
GGCAAGAAGG AAATGTTGGT GGAAGATAAA CTGAGTCTTC TGAATAGTAA CTGGATAGCC 
GTCACTTCCC GAGCAGAAGA GTGGTTAAAC CTTTTATTGG AATACCAGAA ACACATGGAA 
AACTTTGACC AGAATGTGGA TTACATCACA AACTGGATCA TTCAGGCTGA TGCACTTTTG 
GATGAATCTG AGAAAAAGAA ACCTCAGCAA AAAGAAGACA TACTTAAGCG TTTAAAGGCT 
GAAATGAATG ACATACGTCC AAAGGTGGAT TCTACACGTG ACCAAGCAGC AAACCTGATG 
GCAAACCGCG GCGACCACTG CAGGAAAGTA GTAGAGCCCA AAATCTCAGA GCTCAACCAT 
CGATTTGCAG CCATTTCTCA CAGAATTAAG ACTGGAAAGG CCTCCATTCC TTTGAAGGAA 
TTGGAGCAGT TTAACTCAGA TATACAAAAA TTGCTTGAAC CACTGGAGGC TGAAATTCAG 
CAGGGGGTGA ATCTGAAAGA GGAAGACTTC AATAAAGATA TGAGTGAAGA CAATGAGGGT 
ACTGTAAAAG AATTGTTGCA AAGAGGAGAC AACTTACAAC AAAGAATCAC AGATGAGAGA 
AAGCGAGAGG AAATAAAGAT AAAACAACAG CTGTTACAGA CAAAACATAA TGCTCTCAAG 
GATTTGAGGT CTCAAAGAAG AAAAAAGGCT CTAGAAATTT CTCACCAGTG GTATCAGTAC 
AAGAGGCAGG CTGATGATCT CCTGAAATGC TTGGATGACA TTGAAAAAAA ATTAGCCAGC 
CTACCTGAAC CCAGAGATGA AAGGAAAATA AAGGAAATTG ATCGTGAATT GCAGAAGAAG 
AAAGAGGAGC TGAATGCAGT GCGTAGGCAA GCTGAGGGCT TGTCTGAGGA TGGGGCCGCA 
ATGGCAGTGG AGCCAACTCA GATCCAGCTC AGCAAGCGCT GGCGGGAAAT TGAGAGCAAA 
TTTGCTCAGT TTCGAAGACT CAACTTTGCA CAAATTCACA CTGTCCATGA AGAGTCAGTG 
GTGGCGATGA CTGAAGACAT GCCTTTGGAA ATTTCTTATG TGCCTTCTAC TTACCTGACT 
GAGATCACTC ATGTCTCACA AGCCCTATCA GAAGTGGAAG AGCTTCTTAA TGCTCCCGAC 
CTTTGTGCTC AAGATTTTGA AGATCTCTTT AAACAAGAGG AATCCTTGAA GAACATAAAA 
GACAGCCTGC AACAAATCTC AGGTCGGATT GACATCATTC ACAATAAAAA GACAGCAGCA 
TTGCACAGTG CCACTCCTGC AGAAAGGGCA AAGCTCCAGG AAGCTCTCTC ACGGCTTGAT 
TTCCAATGGG AAAGAGTTAA CAATATGTAC AAGGACCGAC AAGGGAGATT TGACAGATCT 
GTGGAAAAAT GGCGGCGGTT TCATTATGAT ATGAAGATAC TTAATCAATG GCTAACAGAA 
GCTGAACAGT TTCTCAAAAA GACACAAATT CCTGAGAATT GGGAACATGC CAAATACAAA 
TGGTATCTTA AGGAACTCCA GGATGGCATT GGACAGCGGC AAAGTGTTGT CAGGGTATTG 
AATGCAACTG GGGAAGAAAT AATTCAACAG TCCTCAAAAA CAGATGCCAG TATTCTCCAA 
GAAAAACTGG GAAGCCTGAA TCTGCGGTGG CAGGAGGTCT GCAAACAGCT GGCAGAAAGA 
AAAAAGAGGC TAGAGGAACA GAAGAATATC TTGTCAGAAT TTCAAAGAGA TGTAAATGAA 
TTTGTTTTAT GGTTGGAAGA AGCGGATAAC GTTGCTAATA TTCCACTTGA ACCTGGAAAT 
GAGCAGCAGC TAAAAGAAAA ACTTGAACAA GTCAAGTTAC TGGCAGAAGA GTTGCCCCTG 
CGCCAGGGAA TTCTAAAACA ATTAAATGAA ACTGGAGGAA CAGTGCTTGT AAGTGCTCCC 
CTAAGCCCAG AAGAGCAAGA TAAACTTGAA AATAAGCTCA AGCAGACAAA TCTTCAGTGG 
ATAAAGGTTT CTAGAAATCT GCCTGAGAAG CAAGAAGAAA TTGAGGCACA CGTAAAAGAC 
CTTGGACAGC TGGAAGAGCA GTTAAATCAT CTGCTTCTAT GGCTGTCTCC TATTAGGAAT 
CAGTTGGAAA TTTACAATCA GCCAAATCAA ACAGGACCAT TTGACATCAA GGAAATTGAA 
GTAGCAGTTC AAGCTAAACA GCCGGATGTG GAAGGGATTT TGTCTAAAGG GCAGCATTTG 
TACAAGGAAA AACCAGCCAC TCAGCCAGCG AAGAGAAAGC TGGAAGATCT CAGCTCTGAT 
TGGAAGGTGG TAACTCAGTT GCTTCAAGAG CTGCGGGCAA AGCAGCCTGG CCCAGCTCCT 
GGACTGACCA CTGTCAGAGC CCCTCCCAGT CAGACTGTTA CTCTGGTGAC ACAACCCGCG 
GTTACCAAGG AAACTGCCAT CTCCAAACTA GAAATGCCAT CTTCATTGCT GTTGGAGGTA 
CCTGCACTGG CAGATTTCAA CCGAGCTTGG ACAGAACTTA CCGACTGGCT GTCTCTGCTT 
GATCGAGTTA TAAAATCACA GAGGGTGATG GTGGGTGATC TTGAAGACAT TAACGAGATG 
ATCATCAAGC AGAAGGCAAC GCTGCAGGAT TTGGAACAGA GGCGCCCCCA GTTGGAAGAA 
CTCATTACCG CTGCCCAGAA TTTGAAAAAC AAGACCAGCA ATCAAGAGGC TAGAACAATC 
ATTACTGATC GAATTGAAAG AATTCAGAGT CAGTGGGATG AAGTACAGGA ACATCTTCAG 
AACCGGAGGC AACAGTTGAA TGAAATGTTA AAGGATTCCA CACAATGGCT GGAAGCTAAA 
GAGGAGGCTG AGCAGGTGTT GGGGCAGGCC AGAGCCAAGC TTGAGTCATG GAAGGAGGCT 
CCCTACACAG TAGATGCAAT CCAAAAGAAA ATCACAGAAA CCAAGCAGTT GGCCAAAGAC 
CTCCGCCAGT GGCAGATAAA TGTAGATGTT GCAAATGATT TGGCACTGAA ACTTCTCCGA 
GATTATTCTG CAGATGATAC CAGAAAAGTA CACATGATAA CAGAGAACAT CAATGCCTCT 
TGGGCAAGCA TCCATAAAAG ATTGAGTGAG CGAGAGGCTG CTCTGGAAGA AACCCACAGA 
TTACTGCAAC AGTTCCCCTT GGACCTGGAG AAGTTCCTTG CCTGGCTTAC AGAAGCCGAA 
ACAACTGCCA ACGTCCTGCA GGATGCCACC CATAAGGAAA GGCTTCTAGA AGATTCCAAG 
GGAGTAAGAG AGCTGATGAA ACAATGGCAA GATCTCCAAG GAGAAATCGA AGCTCACACA 
GATATCTATC ACAACCTGGA CGAAAATGGC CAAAAAGTCC TGAGATCCCT GGAAGGTTCT 
GACGATGCAG CCTTGTTGCA AAGACGTTTG GATAACATGA ACTTCAAGTG GAGCGAACTT 
CGGAAAAAGT CTCTCAACAT TAGGTCTCAC TTGGAAGCCA GTTCTGACCA GTGGAAGCGT 
CTGCACCTTT CTCTTCAGGA ACTTCTGGTA TGGCTCCAGC TGAAAGATGA TGAGTTAAGC 
CGGCAGGCAC CCATTGGAGG AGACTTTCCA GCGGTGCAGA AGCAGAATGA TGTACACAGG 
GCCTTCAAGA GGGAATTGAA AACGAAAGAA CCTGTAATCA TGAGTACTCT TGAGACTGTA 
CGAATATTTC TGACAGAGCA GCCTTTAGAA GGACTAGAGA AACTCTACCA GGAGCCCAGA 
GAGCTGCCTC CTGAAGAGAG AGCCCAGAAT GTCACACGGC TCCTACGAAA GCAAGCTGAG 
GAGGTCAACA CTCAGTGGGA AAAACTGAAC GTGCACTCTG CAGACTGGCA GAGAAAAATA 
GACGAGGCCC TCGAAAGACT CCAGGAGCTT CAGGAAGCAA CAGATGAGCT GGATCTCAAA 
CTACGTCAGG CAGAGGTGAT CAAAGGATCC TGGCAGCCTG TGGGTGATCT CCTCATTGAC 
TCTCTCCAAG ATCACCTCGA AAAAGTCAAG GCGCTTCGAG GAGAAATTAC ACCTCTGAAA 
GAGAATGTCA GCTACGTCAA TGACCTTGCT CGCCAACTCA CTACGTTGGG CATTCAGCTG 
TCACCATATA ACCTCAACAC TCTGGAAGAC CTGAACACCA GATGGAAGCT TCTGCAGGTG 
GCCATTGAGG ACCGCATCAG GCAGCTGCAT GAAGCGCACA GGGACTTTGG ACCAGCCTCC 
CAGCACTTCC TTTCCACTTC TGTCCAGGGT CCCTGGGAGA GAGCCATCTC ACCAAACAAA 
GTGCCCTACT ATATCAACCA CGAGACCCAA ACAACTTGCT GGGACCATCC CAAAATGACA 
GAGCTCTACC AGTCTTTAGC TGACCTGAAT AATGTCAGGT TCTCAGCTTA CAGGACTGCC 
ATGAAACTCC GAAGACTGCA GAAGGCCCTT TGCTTGGATC TCTTGAGCCT ATCGGCTGCA 
TGCGATGCCT TGGACCAGCA CAACCTCAAG CAAAATGACC AGCCCATGGA TATCCTGCAG 
GTCATTAACT GTCTGACCAC TATTTATGAT CGCCTAGAGC AAGAGCACAA CAATCTGGTC 
AACGTCCCTC TCTGCGTGGA TATGTGTCTC AATTGGCTGC TGAATGTTTA TGACACGGGA 
CGAACGGGGA GGATCCGGGT CCTGTCTTTT AAAACTGGCA TCATTTCTCT GTGTAAAGCC 
CATTTGGAAG ACAAGTACAG ATACCTCTTC AAGCAAGTGG CAAGTTCGAC AGGATTTTGT 
GACCAGCGCA GGCTGGGCCT CCTCCTGCAT GACTCTATCC AGATCCCAAG ACAGTTGGGT 
GAAGTCGCAT CGTTCGGGGG CAGTAACATT GAGCCGAGTG TCAGGAGCTG CTTCCAGTTT 
GCTAATAATA AGCCTGAGAT CGAAGCGGCC CTCTTCCTAG ACTGGATGCG CCTGGAGCCC 
CAGTCCATGG TGTGGCTGCC TGTCCTGCAC CGAGTGGCTG CCGCGGAAAC TGCCAAGCAC 
CAGGCCAAGT GCAACATCTG CAAGGAGTGT CCCATCATCG GATTCAGGTA CAGGAGTCTA 
AAGCACTTTA ATTATGACAT CTGCCAAAGT TGCTTTTTTT CTGGTCGAGT TGCAAAAGGC 
CATAAAATGC ACTATCCCAT GGTGGAATAC TGCACTCCGA CTACATCGGG AGAAGATGTC 
CGTGACTTTG CCAAGGTACT AAAAAACAAA TTTCGAACCA AAAGGTATTT TGCGAAGCAT 
CCCCGAATGG GCTACCTGCC AGTGCAGACT GTCTTAGAGG GGGACAACAT GGAAACTCCT 
GTCACTCTGA TCAACTTCTG GCCGGTAGAT TCTGCGCCTG CCTCGTCCCC TCAGCTTTCA 
CACGATGATA CTCATTCACG CATTGAGCAT TATGCTAGCA GGCTAGCAGA AATGGAAAAC 
AGCAATGGAT CTTATCTAAA TGATAGCATC TCTCCTAATG AGAGCATAGA TGATGAACAT 
TTGTTAATCC AGCATTACTG CCAAAGTTTG AACCAGGACT CCCCCCTGAG CCAGCCTCGT 
AGTCCTGCCC AGATCTTGAT TTCCTTAGAG AGTGAGGAAA GAGGGGAGCT AGAGAGAATC 
CTAGCAGATC TTGAGGAAGA AAACAGAAAT CTGCAAGCAG AGTATGATCG TCTAAAGCAG 
CAGCATGAAC ACAAAGGCCT GTCCCCACTG CCATCCCCTC CTGAAATGAT GCCTACTTCT 
CCCCAAAGTC CCCGGGATGC TGAGCTCATC GCTGAGGCCA AGCTGCTGCG TCAACACAAA 
GGCCGCCTGG AAGCCAGGAT GCAAATCCTT GAAGACCATA ACAAACAACT GGAATCCCAG 
TTACACAGGC TCAGGCAGCT GCTGGAGCAA CCCCAGGCAG AGGCCAAGGT GAATGGTACA 
ACGGTGTCTT CTCCTTCTAC CTCTCTTCAG AGGTCAGATA GCAGTCAGCC TATGCTGCTC 
CGGGTGGTCG GCAGTCAGAC TTCAGAATCC ATGGGCGAGG AAGACCTGCT CAGCCCTCCC 
CAGGACACAA GCACAGGGTT AGAGGAAGTG ATGGAGCAGC TCAACCACTC CTTCCCTAGT 
TCCAGAGGAC ACAATGTAGG AAGCCTTTTC CACTTGGCAG ATGATTTGGG CAGAGCGATG 
GAGTCCTTAG TTTCAGTCAT GACAGATGAA GAAGGGGCAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013966818.1
CDS	204..11288
Translation	MSEVSSDEREDVQKKTFTKWINAQFSKFGKQHIENLFSDLQDGRRLLDLLEGLTGQKLPKEKGSTRVHALNNVNKALRVLQKNNVDLVNIGSTDIVDGNHKLTLGLIWNIILHWQVKNVMKNIMAGLQQTNSEKILLSWVRQSTRNYPQVNVINFTTSWSDGLALNALIHSHRPDLFDWNSVVCQQSATQRLEHAFNIAKYQLGIEKLLDPEDVATTYPDKKSILMYITSLFQVLPQQVSIEAIQEVEMLPRPSKVTREEHFQLHHQMHYSQQITVSLAQGYERAPSSPKPRFKSYAYTQAAYVTTSDPTRSPLPSQHLETPEDKSFGRSLTETEANLDSYQTALEEVLSWLLSAEDALQAQGEISNDVEEVKEQFHTHEGYMMDLTSHQGRVGNVLQLGSQLIGTGKLSEDEETEVQEQMNLLNSRWECLRVASMEKQSNLHKVLMDLQNQQLKELNDWLTKTEERTRKMEKEPLGPDIEDLKRQVQQHKVLQEDLEQEQVRVNSLTHMVVVVDESSGDHATAALEEQLKVLGDRWANICRWTEDRWVLLQDILLKWQRFTEEQCLFSAWLSEKEDAVNKIHTTGFKDQSEVLSNLQKLAVLKTDLEKKKQTMDKLCSLNQDLLSALKNTVVAHKMEAWLDNFAQRWDNLVQKLEKSSAQISQAVTTTQPSLTQTTVMETVTMVTTREHILVKHAQEELPPPPPQKKRQIIVDSEIRKRLDVDITELHSWITRSEAVLQSPEFAIYRKEGNFSDLKEKVNAIEREKAEKFRKLQDASRSAQALVEQMVNEGVNADSIKQASEQLNSRWIEFCQLLSERLNWLEYQNNIITFYNQLQQLEQMTTTAENWLKTQPTTTSEPTAIKSQLKICKDEINRLSALQPQIERLKIQSIALKEKGQGPMFLDADFVAFTNHFNQVFADVQAREKELQTIFDSLPPMRYQETMSTILTWIQQSETKLSIPQVTVTEYDIMEQRLGELQALQSSLQEQQNGLNYLSTTVKEMSKKAPLSDISRKYQSEFEEIEGRWKKLSSQLVEHCQKLEEQMAKLRKIQNHIKTLKKWITEVDVFLKEEWPALGDSEILKRQLKQCRLLVNDIQTIQPSLNSVNEGAQKMKNEAEPEFAGRLETELRELNTQWDYMCRQVYARKEALKGGLDKTVSLQKDLSEMHEWMTQAEEEYLERDFEYKTPDELQTAVEEMKRAKEEAQQKEAKVKLLTESVNSVIAQAPPAAQEALKKELDTLTTNYQWLCTRLNGKCKTLEEVWACWHELLSYLEKANKWLSEVEVKLKTTENISGGAEEIAEVLDSLENLMQHSEDNPNQIRILAQTLTDGGVMDELINEELETFNSRWRELHEEAVRRQKLLEQSIQSAQEIEKSLHLIQESLSSIDKQLAAYIADKVDAAQMPQEAQKIQSDLTSHEISLEEMKKHNQGKETAQRVLSQIDVAQKKLQDVSMKFRLFQKPANFEQRLQESKMILDEVKMHLPALETKSVEQEVVQSQLNHCVNLYKSLSEVKSEVEMVIKTGRQIVQKKQTENPKELDERVTALKLHYNELGAKVTERKQQLEKCLKLSRKMRKEMNALTEWLAATDMELTKRSAVEGMPSNLDSEVAWGKATQKEIEKQKVHLKSVTEVGEALKTVLGKKEMLVEDKLSLLNSNWIAVTSRAEEWLNLLLEYQKHMENFDQNVDYITNWIIQADALLDESEKKKPQQKEDILKRLKAEMNDIRPKVDSTRDQAANLMANRGDHCRKVVEPKISELNHRFAAISHRIKTGKASIPLKELEQFNSDIQKLLEPLEAEIQQGVNLKEEDFNKDMSEDNEGTVKELLQRGDNLQQRITDERKREEIKIKQQLLQTKHNALKDLRSQRRKKALEISHQWYQYKRQADDLLKCLDDIEKKLASLPEPRDERKIKEIDRELQKKKEELNAVRRQAEGLSEDGAAMAVEPTQIQLSKRWREIESKFAQFRRLNFAQIHTVHEESVVAMTEDMPLEISYVPSTYLTEITHVSQALSEVEELLNAPDLCAQDFEDLFKQEESLKNIKDSLQQISGRIDIIHNKKTAALHSATPAERAKLQEALSRLDFQWERVNNMYKDRQGRFDRSVEKWRRFHYDMKILNQWLTEAEQFLKKTQIPENWEHAKYKWYLKELQDGIGQRQSVVRVLNATGEEIIQQSSKTDASILQEKLGSLNLRWQEVCKQLAERKKRLEEQKNILSEFQRDVNEFVLWLEEADNVANIPLEPGNEQQLKEKLEQVKLLAEELPLRQGILKQLNETGGTVLVSAPLSPEEQDKLENKLKQTNLQWIKVSRNLPEKQEEIEAHVKDLGQLEEQLNHLLLWLSPIRNQLEIYNQPNQTGPFDIKEIEVAVQAKQPDVEGILSKGQHLYKEKPATQPAKRKLEDLSSDWKVVTQLLQELRAKQPGPAPGLTTVRAPPSQTVTLVTQPAVTKETAISKLEMPSSLLLEVPALADFNRAWTELTDWLSLLDRVIKSQRVMVGDLEDINEMIIKQKATLQDLEQRRPQLEELITAAQNLKNKTSNQEARTIITDRIERIQSQWDEVQEHLQNRRQQLNEMLKDSTQWLEAKEEAEQVLGQARAKLESWKEAPYTVDAIQKKITETKQLAKDLRQWQINVDVANDLALKLLRDYSADDTRKVHMITENINASWASIHKRLSEREAALEETHRLLQQFPLDLEKFLAWLTEAETTANVLQDATHKERLLEDSKGVRELMKQWQDLQGEIEAHTDIYHNLDENGQKVLRSLEGSDDAALLQRRLDNMNFKWSELRKKSLNIRSHLEASSDQWKRLHLSLQELLVWLQLKDDELSRQAPIGGDFPAVQKQNDVHRAFKRELKTKEPVIMSTLETVRIFLTEQPLEGLEKLYQEPRELPPEERAQNVTRLLRKQAEEVNTQWEKLNVHSADWQRKIDEALERLQELQEATDELDLKLRQAEVIKGSWQPVGDLLIDSLQDHLEKVKALRGEITPLKENVSYVNDLARQLTTLGIQLSPYNLNTLEDLNTRWKLLQVAIEDRIRQLHEAHRDFGPASQHFLSTSVQGPWERAISPNKVPYYINHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQVINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPVTLINFWPVDSAPASSPQLSHDDTHSRIEHYASRLAEMENSNGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKQQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDTSTGLEEVMEQLNHSFPSSRGHNVGSLFHLADDLGRAMESLVSVMTDEEGAE

Target ORF information:

RefSeq Version	XM_014111343.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris dystrophin (DMD), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111343.2

ORF Insert Sequence:

ATGTCTGAGG TCTCATCTGA TGAAAGAGAA GATGTTCAAA AGAAAACATT CACAAAATGG 
ATAAATGCAC AGTTTTCTAA GTTTGGGAAG CAGCACATAG AGAACCTCTT CAGTGACCTA 
CAGGATGGGA GACGCCTCCT AGACCTTTTG GAAGGCCTGA CAGGGCAAAA ACTGCCAAAA 
GAAAAAGGAT CCACAAGAGT TCATGCCCTG AACAATGTCA ACAAGGCACT GCGCGTCTTG 
CAGAAAAATA ATGTTGATTT AGTGAACATT GGAAGTACTG ACATAGTAGA TGGAAATCAC 
AAACTGACTC TTGGTTTGAT TTGGAATATA ATCCTCCACT GGCAGGTCAA AAATGTAATG 
AAAAATATCA TGGCTGGATT GCAACAAACC AACAGTGAAA AGATTCTCCT GAGCTGGGTC 
CGACAATCAA CTCGTAATTA TCCACAGGTT AATGTCATTA ACTTCACCAC CAGCTGGTCT 
GATGGCCTGG CTTTGAACGC TCTCATCCAC AGTCATAGGC CAGACCTGTT TGATTGGAAT 
AGTGTGGTTT GCCAGCAGTC AGCCACACAA CGCCTGGAAC ATGCATTCAA CATTGCCAAA 
TATCAATTAG GCATAGAGAA ACTGCTTGAT CCTGAAGATG TTGCCACCAC TTATCCAGAT 
AAGAAGTCCA TCTTAATGTA TATCACATCA CTCTTCCAAG TTTTGCCTCA ACAAGTGAGC 
ATTGAAGCCA TCCAGGAAGT GGAAATGTTG CCAAGGCCAT CAAAAGTTAC TAGAGAAGAA 
CATTTTCAAT TACATCATCA AATGCACTAT TCTCAACAGA TCACAGTCAG TCTAGCACAG 
GGCTATGAAC GAGCCCCTTC CTCTCCTAAG CCTCGGTTCA AGAGCTATGC CTACACACAG 
GCTGCTTATG TCACCACTTC TGACCCCACA CGGAGCCCAC TTCCTTCACA GCATTTGGAA 
ACTCCTGAAG ACAAGTCATT TGGCCGGTCA TTGACAGAGA CCGAAGCAAA CCTGGACAGT 
TATCAAACAG CTTTGGAAGA AGTACTCTCG TGGCTTCTTT CAGCTGAGGA TGCACTGCAA 
GCCCAAGGAG AGATTTCTAA TGATGTCGAA GAAGTGAAAG AACAATTTCA TACTCATGAG 
GGATATATGA TGGACTTGAC ATCCCATCAG GGACGGGTCG GTAATGTTCT CCAACTGGGA 
AGTCAACTGA TTGGAACAGG GAAATTATCA GAAGATGAAG AAACCGAAGT GCAGGAACAA 
ATGAATCTCC TCAATTCAAG ATGGGAATGC CTCAGGGTAG CTAGCATGGA AAAACAAAGC 
AATTTACATA AAGTTCTAAT GGATCTCCAG AATCAGCAAC TGAAAGAGTT AAATGACTGG 
CTAACCAAAA CAGAAGAGAG AACAAGGAAA ATGGAGAAGG AGCCCCTTGG ACCTGATATT 
GAAGACCTAA AACGCCAAGT ACAACAACAT AAGGTGCTTC AAGAAGACTT AGAACAGGAA 
CAAGTCAGGG TCAATTCCCT CACTCATATG GTGGTGGTAG TCGATGAATC TAGTGGAGAC 
CATGCAACTG CTGCTTTGGA AGAACAACTT AAGGTACTGG GAGATCGATG GGCAAACATC 
TGTAGGTGGA CAGAAGATCG CTGGGTTCTT TTACAAGACA TCCTCCTAAA ATGGCAGCGT 
TTTACTGAAG AACAGTGCCT TTTCAGTGCA TGGCTTTCGG AGAAGGAAGA TGCAGTGAAC 
AAGATTCACA CAACTGGCTT TAAGGATCAA AGTGAAGTGT TATCAAATCT TCAGAAACTG 
GCTGTCTTAA AAACAGATCT GGAAAAGAAG AAGCAAACCA TGGACAAACT CTGCTCACTC 
AACCAAGACC TTCTTTCAGC GCTGAAAAAC ACAGTGGTAG CCCACAAGAT GGAAGCATGG 
CTGGACAACT TTGCCCAGCG CTGGGATAAT TTAGTCCAGA AACTTGAAAA AAGTTCAGCA 
CAGATTTCAC AGGCTGTCAC CACCACTCAG CCATCACTAA CACAGACAAC TGTAATGGAA 
ACAGTAACTA TGGTGACCAC GAGGGAACAC ATCTTGGTAA AGCATGCCCA AGAGGAACTG 
CCACCACCAC CCCCTCAGAA GAAGAGGCAG ATTATCGTGG ATTCTGAAAT TAGGAAAAGG 
TTGGATGTCG ATATAACTGA ACTTCACAGT TGGATTACTC GTTCAGAAGC TGTGTTGCAG 
AGTCCTGAAT TTGCAATCTA TCGGAAGGAA GGCAACTTCT CAGACCTTAA AGAAAAAGTC 
AATGCCATAG AGCGAGAAAA AGCCGAGAAG TTCAGAAAAC TGCAAGATGC CAGCAGATCA 
GCTCAGGCCC TGGTGGAACA GATGGTGAAT GAGGGTGTTA ATGCTGACAG CATCAAACAA 
GCCTCCGAAC AACTGAACAG CCGGTGGATA GAGTTCTGCC AATTGCTAAG CGAGAGACTT 
AACTGGCTGG AGTATCAGAA CAACATCATC ACTTTCTATA ATCAGCTACA ACAATTGGAG 
CAGATGACAA CTACTGCTGA AAACTGGTTG AAAACCCAGC CTACCACCAC ATCAGAGCCA 
ACAGCAATTA AAAGCCAGTT AAAAATTTGT AAGGATGAAA TCAACCGACT GTCAGCTCTT 
CAGCCTCAAA TCGAGCGATT AAAAATTCAA AGCATAGCCC TGAAAGAGAA AGGACAAGGG 
CCAATGTTCC TGGATGCAGA CTTTGTGGCC TTTACAAATC ATTTTAACCA AGTCTTTGCT 
GATGTGCAGG CAAGAGAAAA AGAGCTACAA ACAATTTTTG ACAGTTTGCC ACCCATGCGC 
TATCAGGAGA CTATGAGTAC CATCCTGACA TGGATCCAGC AGTCAGAAAC CAAACTCTCT 
ATACCTCAGG TTACTGTCAC TGAATATGAC ATCATGGAAC AGAGACTCGG AGAGCTACAG 
GCTTTACAAA GCTCTCTGCA AGAGCAACAA AATGGCCTAA ACTATCTCAG CACCACTGTG 
AAAGAGATGT CAAAGAAAGC ACCACTGTCT GATATTAGTC GGAAATATCA ATCAGAATTT 
GAAGAGATTG AGGGACGTTG GAAGAAGCTG TCTTCCCAGC TGGTTGAACA TTGTCAAAAG 
TTGGAGGAGC AAATGGCTAA ACTTCGAAAA ATTCAGAATC ACATAAAAAC TCTGAAGAAA 
TGGATCACTG AAGTCGATGT TTTCCTGAAG GAGGAATGGC CTGCCCTTGG GGATTCAGAA 
ATTCTGAAAA GACAGCTGAA ACAGTGCAGG CTTTTAGTCA ATGACATTCA GACCATCCAG 
CCTAGTCTCA ACAGTGTCAA TGAAGGGGCT CAGAAGATGA AGAATGAAGC AGAACCAGAG 
TTTGCTGGCA GACTTGAGAC AGAGCTCCGA GAACTTAACA CCCAGTGGGA TTACATGTGC 
CGCCAGGTCT ATGCCAGGAA GGAAGCCTTA AAAGGAGGTT TGGATAAAAC TGTAAGTCTT 
CAGAAAGATC TGTCAGAGAT GCATGAGTGG ATGACACAAG CTGAAGAAGA ATACCTAGAG 
AGAGATTTCG AATACAAGAC CCCTGATGAA TTACAGACAG CAGTTGAAGA GATGAAGAGA 
GCTAAAGAAG AGGCCCAGCA AAAAGAAGCA AAAGTGAAAC TCCTAACCGA GTCCGTCAAT 
AGTGTCATAG CTCAGGCTCC ACCTGCAGCA CAAGAGGCCT TAAAAAAGGA ACTTGACACT 
CTCACCACCA ACTACCAGTG GCTCTGCACC AGGCTCAATG GCAAATGCAA GACCTTGGAA 
GAAGTTTGGG CGTGCTGGCA TGAGTTATTG TCCTACTTGG AGAAGGCAAA CAAGTGGCTA 
AGTGAAGTAG AAGTCAAGCT TAAAACCACT GAAAATATTT CTGGGGGAGC TGAGGAAATC 
GCCGAGGTGC TTGATTCGCT TGAAAATTTG ATGCAACATT CAGAGGATAA CCCGAATCAG 
ATTCGCATAT TGGCACAGAC CTTGACAGAT GGTGGAGTCA TGGATGAACT GATCAATGAG 
GAGCTTGAGA CATTTAATTC TCGTTGGAGA GAACTCCATG AAGAGGCTGT GAGGAGGCAA 
AAGTTGCTTG AGCAGAGTAT CCAGTCGGCC CAGGAGATAG AAAAATCCTT GCACTTAATT 
CAGGAGTCCC TCTCTTCCAT TGACAAGCAG TTGGCAGCTT ATATTGCTGA CAAAGTGGAT 
GCAGCTCAGA TGCCTCAGGA AGCCCAGAAA ATCCAATCAG ATTTGACAAG TCATGAGATC 
AGTTTAGAAG AAATGAAGAA ACATAACCAG GGAAAGGAGA CTGCCCAAAG GGTACTATCC 
CAAATTGATG TGGCACAGAA AAAATTGCAG GATGTTTCCA TGAAGTTTCG ATTATTCCAG 
AAGCCAGCCA ATTTTGAGCA GCGCCTACAA GAAAGTAAAA TGATTTTAGA TGAAGTGAAG 
ATGCATTTAC CTGCGTTGGA AACAAAGAGT GTGGAACAGG AAGTAGTACA GTCACAGTTA 
AATCATTGTG TGAACTTGTA TAAAAGTCTG AGTGAAGTGA AGTCTGAAGT GGAAATGGTA 
ATAAAAACTG GACGTCAGAT TGTACAGAAG AAGCAGACGG AAAACCCGAA AGAGCTTGAT 
GAAAGAGTTA CAGCTTTGAA ATTGCATTAT AATGAGCTGG GAGCAAAGGT GACAGAAAGA 
AAGCAACAGT TGGAAAAATG CTTGAAATTG TCCCGTAAGA TGCGAAAGGA AATGAATGCC 
CTGACAGAAT GGCTGGCAGC GACAGATATG GAACTGACGA AGAGATCGGC AGTTGAAGGA 
ATGCCTAGTA ATTTGGATTC TGAAGTTGCC TGGGGAAAGG CTACTCAGAA AGAGATTGAG 
AAACAGAAGG TTCACCTAAA GAGTGTCACA GAGGTAGGAG AGGCCTTGAA AACGGTTTTG 
GGCAAGAAGG AAATGTTGGT GGAAGATAAA CTGAGTCTTC TGAATAGTAA CTGGATAGCC 
GTCACTTCCC GAGCAGAAGA GTGGTTAAAC CTTTTATTGG AATACCAGAA ACACATGGAA 
AACTTTGACC AGAATGTGGA TTACATCACA AACTGGATCA TTCAGGCTGA TGCACTTTTG 
GATGAATCTG AGAAAAAGAA ACCTCAGCAA AAAGAAGACA TACTTAAGCG TTTAAAGGCT 
GAAATGAATG ACATACGTCC AAAGGTGGAT TCTACACGTG ACCAAGCAGC AAACCTGATG 
GCAAACCGCG GCGACCACTG CAGGAAAGTA GTAGAGCCCA AAATCTCAGA GCTCAACCAT 
CGATTTGCAG CCATTTCTCA CAGAATTAAG ACTGGAAAGG CCTCCATTCC TTTGAAGGAA 
TTGGAGCAGT TTAACTCAGA TATACAAAAA TTGCTTGAAC CACTGGAGGC TGAAATTCAG 
CAGGGGGTGA ATCTGAAAGA GGAAGACTTC AATAAAGATA TGAGTGAAGA CAATGAGGGT 
ACTGTAAAAG AATTGTTGCA AAGAGGAGAC AACTTACAAC AAAGAATCAC AGATGAGAGA 
AAGCGAGAGG AAATAAAGAT AAAACAACAG CTGTTACAGA CAAAACATAA TGCTCTCAAG 
GATTTGAGGT CTCAAAGAAG AAAAAAGGCT CTAGAAATTT CTCACCAGTG GTATCAGTAC 
AAGAGGCAGG CTGATGATCT CCTGAAATGC TTGGATGACA TTGAAAAAAA ATTAGCCAGC 
CTACCTGAAC CCAGAGATGA AAGGAAAATA AAGGAAATTG ATCGTGAATT GCAGAAGAAG 
AAAGAGGAGC TGAATGCAGT GCGTAGGCAA GCTGAGGGCT TGTCTGAGGA TGGGGCCGCA 
ATGGCAGTGG AGCCAACTCA GATCCAGCTC AGCAAGCGCT GGCGGGAAAT TGAGAGCAAA 
TTTGCTCAGT TTCGAAGACT CAACTTTGCA CAAATTCACA CTGTCCATGA AGAGTCAGTG 
GTGGCGATGA CTGAAGACAT GCCTTTGGAA ATTTCTTATG TGCCTTCTAC TTACCTGACT 
GAGATCACTC ATGTCTCACA AGCCCTATCA GAAGTGGAAG AGCTTCTTAA TGCTCCCGAC 
CTTTGTGCTC AAGATTTTGA AGATCTCTTT AAACAAGAGG AATCCTTGAA GAACATAAAA 
GACAGCCTGC AACAAATCTC AGGTCGGATT GACATCATTC ACAATAAAAA GACAGCAGCA 
TTGCACAGTG CCACTCCTGC AGAAAGGGCA AAGCTCCAGG AAGCTCTCTC ACGGCTTGAT 
TTCCAATGGG AAAGAGTTAA CAATATGTAC AAGGACCGAC AAGGGAGATT TGACAGATCT 
GTGGAAAAAT GGCGGCGGTT TCATTATGAT ATGAAGATAC TTAATCAATG GCTAACAGAA 
GCTGAACAGT TTCTCAAAAA GACACAAATT CCTGAGAATT GGGAACATGC CAAATACAAA 
TGGTATCTTA AGGAACTCCA GGATGGCATT GGACAGCGGC AAAGTGTTGT CAGGGTATTG 
AATGCAACTG GGGAAGAAAT AATTCAACAG TCCTCAAAAA CAGATGCCAG TATTCTCCAA 
GAAAAACTGG GAAGCCTGAA TCTGCGGTGG CAGGAGGTCT GCAAACAGCT GGCAGAAAGA 
AAAAAGAGGC TAGAGGAACA GAAGAATATC TTGTCAGAAT TTCAAAGAGA TGTAAATGAA 
TTTGTTTTAT GGTTGGAAGA AGCGGATAAC GTTGCTAATA TTCCACTTGA ACCTGGAAAT 
GAGCAGCAGC TAAAAGAAAA ACTTGAACAA GTCAAGTTAC TGGCAGAAGA GTTGCCCCTG 
CGCCAGGGAA TTCTAAAACA ATTAAATGAA ACTGGAGGAA CAGTGCTTGT AAGTGCTCCC 
CTAAGCCCAG AAGAGCAAGA TAAACTTGAA AATAAGCTCA AGCAGACAAA TCTTCAGTGG 
ATAAAGGTTT CTAGAAATCT GCCTGAGAAG CAAGAAGAAA TTGAGGCACA CGTAAAAGAC 
CTTGGACAGC TGGAAGAGCA GTTAAATCAT CTGCTTCTAT GGCTGTCTCC TATTAGGAAT 
CAGTTGGAAA TTTACAATCA GCCAAATCAA ACAGGACCAT TTGACATCAA GGAAATTGAA 
GTAGCAGTTC AAGCTAAACA GCCGGATGTG GAAGGGATTT TGTCTAAAGG GCAGCATTTG 
TACAAGGAAA AACCAGCCAC TCAGCCAGCG AAGAGAAAGC TGGAAGATCT CAGCTCTGAT 
TGGAAGGTGG TAACTCAGTT GCTTCAAGAG CTGCGGGCAA AGCAGCCTGG CCCAGCTCCT 
GGACTGACCA CTGTCAGAGC CCCTCCCAGT CAGACTGTTA CTCTGGTGAC ACAACCCGCG 
GTTACCAAGG AAACTGCCAT CTCCAAACTA GAAATGCCAT CTTCATTGCT GTTGGAGGTA 
CCTGCACTGG CAGATTTCAA CCGAGCTTGG ACAGAACTTA CCGACTGGCT GTCTCTGCTT 
GATCGAGTTA TAAAATCACA GAGGGTGATG GTGGGTGATC TTGAAGACAT TAACGAGATG 
ATCATCAAGC AGAAGGCAAC GCTGCAGGAT TTGGAACAGA GGCGCCCCCA GTTGGAAGAA 
CTCATTACCG CTGCCCAGAA TTTGAAAAAC AAGACCAGCA ATCAAGAGGC TAGAACAATC 
ATTACTGATC GAATTGAAAG AATTCAGAGT CAGTGGGATG AAGTACAGGA ACATCTTCAG 
AACCGGAGGC AACAGTTGAA TGAAATGTTA AAGGATTCCA CACAATGGCT GGAAGCTAAA 
GAGGAGGCTG AGCAGGTGTT GGGGCAGGCC AGAGCCAAGC TTGAGTCATG GAAGGAGGCT 
CCCTACACAG TAGATGCAAT CCAAAAGAAA ATCACAGAAA CCAAGCAGTT GGCCAAAGAC 
CTCCGCCAGT GGCAGATAAA TGTAGATGTT GCAAATGATT TGGCACTGAA ACTTCTCCGA 
GATTATTCTG CAGATGATAC CAGAAAAGTA CACATGATAA CAGAGAACAT CAATGCCTCT 
TGGGCAAGCA TCCATAAAAG ATTGAGTGAG CGAGAGGCTG CTCTGGAAGA AACCCACAGA 
TTACTGCAAC AGTTCCCCTT GGACCTGGAG AAGTTCCTTG CCTGGCTTAC AGAAGCCGAA 
ACAACTGCCA ACGTCCTGCA GGATGCCACC CATAAGGAAA GGCTTCTAGA AGATTCCAAG 
GGAGTAAGAG AGCTGATGAA ACAATGGCAA GATCTCCAAG GAGAAATCGA AGCTCACACA 
GATATCTATC ACAACCTGGA CGAAAATGGC CAAAAAGTCC TGAGATCCCT GGAAGGTTCT 
GACGATGCAG CCTTGTTGCA AAGACGTTTG GATAACATGA ACTTCAAGTG GAGCGAACTT 
CGGAAAAAGT CTCTCAACAT TAGGTCTCAC TTGGAAGCCA GTTCTGACCA GTGGAAGCGT 
CTGCACCTTT CTCTTCAGGA ACTTCTGGTA TGGCTCCAGC TGAAAGATGA TGAGTTAAGC 
CGGCAGGCAC CCATTGGAGG AGACTTTCCA GCGGTGCAGA AGCAGAATGA TGTACACAGG 
GCCTTCAAGA GGGAATTGAA AACGAAAGAA CCTGTAATCA TGAGTACTCT TGAGACTGTA 
CGAATATTTC TGACAGAGCA GCCTTTAGAA GGACTAGAGA AACTCTACCA GGAGCCCAGA 
GAGCTGCCTC CTGAAGAGAG AGCCCAGAAT GTCACACGGC TCCTACGAAA GCAAGCTGAG 
GAGGTCAACA CTCAGTGGGA AAAACTGAAC GTGCACTCTG CAGACTGGCA GAGAAAAATA 
GACGAGGCCC TCGAAAGACT CCAGGAGCTT CAGGAAGCAA CAGATGAGCT GGATCTCAAA 
CTACGTCAGG CAGAGGTGAT CAAAGGATCC TGGCAGCCTG TGGGTGATCT CCTCATTGAC 
TCTCTCCAAG ATCACCTCGA AAAAGTCAAG GCGCTTCGAG GAGAAATTAC ACCTCTGAAA 
GAGAATGTCA GCTACGTCAA TGACCTTGCT CGCCAACTCA CTACGTTGGG CATTCAGCTG 
TCACCATATA ACCTCAACAC TCTGGAAGAC CTGAACACCA GATGGAAGCT TCTGCAGGTG 
GCCATTGAGG ACCGCATCAG GCAGCTGCAT GAAGCGCACA GGGACTTTGG ACCAGCCTCC 
CAGCACTTCC TTTCCACTTC TGTCCAGGGT CCCTGGGAGA GAGCCATCTC ACCAAACAAA 
GTGCCCTACT ATATCAACCA CGAGACCCAA ACAACTTGCT GGGACCATCC CAAAATGACA 
GAGCTCTACC AGTCTTTAGC TGACCTGAAT AATGTCAGGT TCTCAGCTTA CAGGACTGCC 
ATGAAACTCC GAAGACTGCA GAAGGCCCTT TGCTTGGATC TCTTGAGCCT ATCGGCTGCA 
TGCGATGCCT TGGACCAGCA CAACCTCAAG CAAAATGACC AGCCCATGGA TATCCTGCAG 
GTCATTAACT GTCTGACCAC TATTTATGAT CGCCTAGAGC AAGAGCACAA CAATCTGGTC 
AACGTCCCTC TCTGCGTGGA TATGTGTCTC AATTGGCTGC TGAATGTTTA TGACACGGGA 
CGAACGGGGA GGATCCGGGT CCTGTCTTTT AAAACTGGCA TCATTTCTCT GTGTAAAGCC 
CATTTGGAAG ACAAGTACAG ATACCTCTTC AAGCAAGTGG CAAGTTCGAC AGGATTTTGT 
GACCAGCGCA GGCTGGGCCT CCTCCTGCAT GACTCTATCC AGATCCCAAG ACAGTTGGGT 
GAAGTCGCAT CGTTCGGGGG CAGTAACATT GAGCCGAGTG TCAGGAGCTG CTTCCAGTTT 
GCTAATAATA AGCCTGAGAT CGAAGCGGCC CTCTTCCTAG ACTGGATGCG CCTGGAGCCC 
CAGTCCATGG TGTGGCTGCC TGTCCTGCAC CGAGTGGCTG CCGCGGAAAC TGCCAAGCAC 
CAGGCCAAGT GCAACATCTG CAAGGAGTGT CCCATCATCG GATTCAGGTA CAGGAGTCTA 
AAGCACTTTA ATTATGACAT CTGCCAAAGT TGCTTTTTTT CTGGTCGAGT TGCAAAAGGC 
CATAAAATGC ACTATCCCAT GGTGGAATAC TGCACTCCGA CTACATCGGG AGAAGATGTC 
CGTGACTTTG CCAAGGTACT AAAAAACAAA TTTCGAACCA AAAGGTATTT TGCGAAGCAT 
CCCCGAATGG GCTACCTGCC AGTGCAGACT GTCTTAGAGG GGGACAACAT GGAAACTCCT 
GTCACTCTGA TCAACTTCTG GCCGGTAGAT TCTGCGCCTG CCTCGTCCCC TCAGCTTTCA 
CACGATGATA CTCATTCACG CATTGAGCAT TATGCTAGCA GGCTAGCAGA AATGGAAAAC 
AGCAATGGAT CTTATCTAAA TGATAGCATC TCTCCTAATG AGAGCATAGA TGATGAACAT 
TTGTTAATCC AGCATTACTG CCAAAGTTTG AACCAGGACT CCCCCCTGAG CCAGCCTCGT 
AGTCCTGCCC AGATCTTGAT TTCCTTAGAG AGTGAGGAAA GAGGGGAGCT AGAGAGAATC 
CTAGCAGATC TTGAGGAAGA AAACAGAAAT CTGCAAGCAG AGTATGATCG TCTAAAGCAG 
CAGCATGAAC ACAAAGGCCT GTCCCCACTG CCATCCCCTC CTGAAATGAT GCCTACTTCT 
CCCCAAAGTC CCCGGGATGC TGAGCTCATC GCTGAGGCCA AGCTGCTGCG TCAACACAAA 
GGCCGCCTGG AAGCCAGGAT GCAAATCCTT GAAGACCATA ACAAACAACT GGAATCCCAG 
TTACACAGGC TCAGGCAGCT GCTGGAGCAA CCCCAGGCAG AGGCCAAGGT GAATGGTACA 
ACGGTGTCTT CTCCTTCTAC CTCTCTTCAG AGGTCAGATA GCAGTCAGCC TATGCTGCTC 
CGGGTGGTCG GCAGTCAGAC TTCAGAATCC ATGGGCGAGG AAGACCTGCT CAGCCCTCCC 
CAGGACACAA GCACAGGGTT AGAGGAAGTG ATGGAGCAGC TCAACCACTC CTTCCCTAGT 
TCCAGAGGAC ACAATGTAGG AAGCCTTTTC CACTTGGCAG ATGATTTGGG CAGAGCGATG 
GAGTCCTTAG TTTCAGTCAT GACAGATGAA GAAGGGGCAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMD ( XM_014111343.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013966818.1 Canis lupus familiaris dystrophin (DMD), transcript variant X3, mRNA.

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CloneID	OCe45414
Clone ID Related Accession (Same CDS sequence)	XM_014111343.2 , XM_014111343.1
Accession Version	XM_014111343.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	dystrophin isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGAGG TCTCATCTGA TGAAAGAGAA GATGTTCAAA AGAAAACATT CACAAAATGG 
ATAAATGCAC AGTTTTCTAA GTTTGGGAAG CAGCACATAG AGAACCTCTT CAGTGACCTA 
CAGGATGGGA GACGCCTCCT AGACCTTTTG GAAGGCCTGA CAGGGCAAAA ACTGCCAAAA 
GAAAAAGGAT CCACAAGAGT TCATGCCCTG AACAATGTCA ACAAGGCACT GCGCGTCTTG 
CAGAAAAATA ATGTTGATTT AGTGAACATT GGAAGTACTG ACATAGTAGA TGGAAATCAC 
AAACTGACTC TTGGTTTGAT TTGGAATATA ATCCTCCACT GGCAGGTCAA AAATGTAATG 
AAAAATATCA TGGCTGGATT GCAACAAACC AACAGTGAAA AGATTCTCCT GAGCTGGGTC 
CGACAATCAA CTCGTAATTA TCCACAGGTT AATGTCATTA ACTTCACCAC CAGCTGGTCT 
GATGGCCTGG CTTTGAACGC TCTCATCCAC AGTCATAGGC CAGACCTGTT TGATTGGAAT 
AGTGTGGTTT GCCAGCAGTC AGCCACACAA CGCCTGGAAC ATGCATTCAA CATTGCCAAA 
TATCAATTAG GCATAGAGAA ACTGCTTGAT CCTGAAGATG TTGCCACCAC TTATCCAGAT 
AAGAAGTCCA TCTTAATGTA TATCACATCA CTCTTCCAAG TTTTGCCTCA ACAAGTGAGC 
ATTGAAGCCA TCCAGGAAGT GGAAATGTTG CCAAGGCCAT CAAAAGTTAC TAGAGAAGAA 
CATTTTCAAT TACATCATCA AATGCACTAT TCTCAACAGA TCACAGTCAG TCTAGCACAG 
GGCTATGAAC GAGCCCCTTC CTCTCCTAAG CCTCGGTTCA AGAGCTATGC CTACACACAG 
GCTGCTTATG TCACCACTTC TGACCCCACA CGGAGCCCAC TTCCTTCACA GCATTTGGAA 
ACTCCTGAAG ACAAGTCATT TGGCCGGTCA TTGACAGAGA CCGAAGCAAA CCTGGACAGT 
TATCAAACAG CTTTGGAAGA AGTACTCTCG TGGCTTCTTT CAGCTGAGGA TGCACTGCAA 
GCCCAAGGAG AGATTTCTAA TGATGTCGAA GAAGTGAAAG AACAATTTCA TACTCATGAG 
GGATATATGA TGGACTTGAC ATCCCATCAG GGACGGGTCG GTAATGTTCT CCAACTGGGA 
AGTCAACTGA TTGGAACAGG GAAATTATCA GAAGATGAAG AAACCGAAGT GCAGGAACAA 
ATGAATCTCC TCAATTCAAG ATGGGAATGC CTCAGGGTAG CTAGCATGGA AAAACAAAGC 
AATTTACATA AAGTTCTAAT GGATCTCCAG AATCAGCAAC TGAAAGAGTT AAATGACTGG 
CTAACCAAAA CAGAAGAGAG AACAAGGAAA ATGGAGAAGG AGCCCCTTGG ACCTGATATT 
GAAGACCTAA AACGCCAAGT ACAACAACAT AAGGTGCTTC AAGAAGACTT AGAACAGGAA 
CAAGTCAGGG TCAATTCCCT CACTCATATG GTGGTGGTAG TCGATGAATC TAGTGGAGAC 
CATGCAACTG CTGCTTTGGA AGAACAACTT AAGGTACTGG GAGATCGATG GGCAAACATC 
TGTAGGTGGA CAGAAGATCG CTGGGTTCTT TTACAAGACA TCCTCCTAAA ATGGCAGCGT 
TTTACTGAAG AACAGTGCCT TTTCAGTGCA TGGCTTTCGG AGAAGGAAGA TGCAGTGAAC 
AAGATTCACA CAACTGGCTT TAAGGATCAA AGTGAAGTGT TATCAAATCT TCAGAAACTG 
GCTGTCTTAA AAACAGATCT GGAAAAGAAG AAGCAAACCA TGGACAAACT CTGCTCACTC 
AACCAAGACC TTCTTTCAGC GCTGAAAAAC ACAGTGGTAG CCCACAAGAT GGAAGCATGG 
CTGGACAACT TTGCCCAGCG CTGGGATAAT TTAGTCCAGA AACTTGAAAA AAGTTCAGCA 
CAGATTTCAC AGGCTGTCAC CACCACTCAG CCATCACTAA CACAGACAAC TGTAATGGAA 
ACAGTAACTA TGGTGACCAC GAGGGAACAC ATCTTGGTAA AGCATGCCCA AGAGGAACTG 
CCACCACCAC CCCCTCAGAA GAAGAGGCAG ATTATCGTGG ATTCTGAAAT TAGGAAAAGG 
TTGGATGTCG ATATAACTGA ACTTCACAGT TGGATTACTC GTTCAGAAGC TGTGTTGCAG 
AGTCCTGAAT TTGCAATCTA TCGGAAGGAA GGCAACTTCT CAGACCTTAA AGAAAAAGTC 
AATGCCATAG AGCGAGAAAA AGCCGAGAAG TTCAGAAAAC TGCAAGATGC CAGCAGATCA 
GCTCAGGCCC TGGTGGAACA GATGGTGAAT GAGGGTGTTA ATGCTGACAG CATCAAACAA 
GCCTCCGAAC AACTGAACAG CCGGTGGATA GAGTTCTGCC AATTGCTAAG CGAGAGACTT 
AACTGGCTGG AGTATCAGAA CAACATCATC ACTTTCTATA ATCAGCTACA ACAATTGGAG 
CAGATGACAA CTACTGCTGA AAACTGGTTG AAAACCCAGC CTACCACCAC ATCAGAGCCA 
ACAGCAATTA AAAGCCAGTT AAAAATTTGT AAGGATGAAA TCAACCGACT GTCAGCTCTT 
CAGCCTCAAA TCGAGCGATT AAAAATTCAA AGCATAGCCC TGAAAGAGAA AGGACAAGGG 
CCAATGTTCC TGGATGCAGA CTTTGTGGCC TTTACAAATC ATTTTAACCA AGTCTTTGCT 
GATGTGCAGG CAAGAGAAAA AGAGCTACAA ACAATTTTTG ACAGTTTGCC ACCCATGCGC 
TATCAGGAGA CTATGAGTAC CATCCTGACA TGGATCCAGC AGTCAGAAAC CAAACTCTCT 
ATACCTCAGG TTACTGTCAC TGAATATGAC ATCATGGAAC AGAGACTCGG AGAGCTACAG 
GCTTTACAAA GCTCTCTGCA AGAGCAACAA AATGGCCTAA ACTATCTCAG CACCACTGTG 
AAAGAGATGT CAAAGAAAGC ACCACTGTCT GATATTAGTC GGAAATATCA ATCAGAATTT 
GAAGAGATTG AGGGACGTTG GAAGAAGCTG TCTTCCCAGC TGGTTGAACA TTGTCAAAAG 
TTGGAGGAGC AAATGGCTAA ACTTCGAAAA ATTCAGAATC ACATAAAAAC TCTGAAGAAA 
TGGATCACTG AAGTCGATGT TTTCCTGAAG GAGGAATGGC CTGCCCTTGG GGATTCAGAA 
ATTCTGAAAA GACAGCTGAA ACAGTGCAGG CTTTTAGTCA ATGACATTCA GACCATCCAG 
CCTAGTCTCA ACAGTGTCAA TGAAGGGGCT CAGAAGATGA AGAATGAAGC AGAACCAGAG 
TTTGCTGGCA GACTTGAGAC AGAGCTCCGA GAACTTAACA CCCAGTGGGA TTACATGTGC 
CGCCAGGTCT ATGCCAGGAA GGAAGCCTTA AAAGGAGGTT TGGATAAAAC TGTAAGTCTT 
CAGAAAGATC TGTCAGAGAT GCATGAGTGG ATGACACAAG CTGAAGAAGA ATACCTAGAG 
AGAGATTTCG AATACAAGAC CCCTGATGAA TTACAGACAG CAGTTGAAGA GATGAAGAGA 
GCTAAAGAAG AGGCCCAGCA AAAAGAAGCA AAAGTGAAAC TCCTAACCGA GTCCGTCAAT 
AGTGTCATAG CTCAGGCTCC ACCTGCAGCA CAAGAGGCCT TAAAAAAGGA ACTTGACACT 
CTCACCACCA ACTACCAGTG GCTCTGCACC AGGCTCAATG GCAAATGCAA GACCTTGGAA 
GAAGTTTGGG CGTGCTGGCA TGAGTTATTG TCCTACTTGG AGAAGGCAAA CAAGTGGCTA 
AGTGAAGTAG AAGTCAAGCT TAAAACCACT GAAAATATTT CTGGGGGAGC TGAGGAAATC 
GCCGAGGTGC TTGATTCGCT TGAAAATTTG ATGCAACATT CAGAGGATAA CCCGAATCAG 
ATTCGCATAT TGGCACAGAC CTTGACAGAT GGTGGAGTCA TGGATGAACT GATCAATGAG 
GAGCTTGAGA CATTTAATTC TCGTTGGAGA GAACTCCATG AAGAGGCTGT GAGGAGGCAA 
AAGTTGCTTG AGCAGAGTAT CCAGTCGGCC CAGGAGATAG AAAAATCCTT GCACTTAATT 
CAGGAGTCCC TCTCTTCCAT TGACAAGCAG TTGGCAGCTT ATATTGCTGA CAAAGTGGAT 
GCAGCTCAGA TGCCTCAGGA AGCCCAGAAA ATCCAATCAG ATTTGACAAG TCATGAGATC 
AGTTTAGAAG AAATGAAGAA ACATAACCAG GGAAAGGAGA CTGCCCAAAG GGTACTATCC 
CAAATTGATG TGGCACAGAA AAAATTGCAG GATGTTTCCA TGAAGTTTCG ATTATTCCAG 
AAGCCAGCCA ATTTTGAGCA GCGCCTACAA GAAAGTAAAA TGATTTTAGA TGAAGTGAAG 
ATGCATTTAC CTGCGTTGGA AACAAAGAGT GTGGAACAGG AAGTAGTACA GTCACAGTTA 
AATCATTGTG TGAACTTGTA TAAAAGTCTG AGTGAAGTGA AGTCTGAAGT GGAAATGGTA 
ATAAAAACTG GACGTCAGAT TGTACAGAAG AAGCAGACGG AAAACCCGAA AGAGCTTGAT 
GAAAGAGTTA CAGCTTTGAA ATTGCATTAT AATGAGCTGG GAGCAAAGGT GACAGAAAGA 
AAGCAACAGT TGGAAAAATG CTTGAAATTG TCCCGTAAGA TGCGAAAGGA AATGAATGCC 
CTGACAGAAT GGCTGGCAGC GACAGATATG GAACTGACGA AGAGATCGGC AGTTGAAGGA 
ATGCCTAGTA ATTTGGATTC TGAAGTTGCC TGGGGAAAGG CTACTCAGAA AGAGATTGAG 
AAACAGAAGG TTCACCTAAA GAGTGTCACA GAGGTAGGAG AGGCCTTGAA AACGGTTTTG 
GGCAAGAAGG AAATGTTGGT GGAAGATAAA CTGAGTCTTC TGAATAGTAA CTGGATAGCC 
GTCACTTCCC GAGCAGAAGA GTGGTTAAAC CTTTTATTGG AATACCAGAA ACACATGGAA 
AACTTTGACC AGAATGTGGA TTACATCACA AACTGGATCA TTCAGGCTGA TGCACTTTTG 
GATGAATCTG AGAAAAAGAA ACCTCAGCAA AAAGAAGACA TACTTAAGCG TTTAAAGGCT 
GAAATGAATG ACATACGTCC AAAGGTGGAT TCTACACGTG ACCAAGCAGC AAACCTGATG 
GCAAACCGCG GCGACCACTG CAGGAAAGTA GTAGAGCCCA AAATCTCAGA GCTCAACCAT 
CGATTTGCAG CCATTTCTCA CAGAATTAAG ACTGGAAAGG CCTCCATTCC TTTGAAGGAA 
TTGGAGCAGT TTAACTCAGA TATACAAAAA TTGCTTGAAC CACTGGAGGC TGAAATTCAG 
CAGGGGGTGA ATCTGAAAGA GGAAGACTTC AATAAAGATA TGAGTGAAGA CAATGAGGGT 
ACTGTAAAAG AATTGTTGCA AAGAGGAGAC AACTTACAAC AAAGAATCAC AGATGAGAGA 
AAGCGAGAGG AAATAAAGAT AAAACAACAG CTGTTACAGA CAAAACATAA TGCTCTCAAG 
GATTTGAGGT CTCAAAGAAG AAAAAAGGCT CTAGAAATTT CTCACCAGTG GTATCAGTAC 
AAGAGGCAGG CTGATGATCT CCTGAAATGC TTGGATGACA TTGAAAAAAA ATTAGCCAGC 
CTACCTGAAC CCAGAGATGA AAGGAAAATA AAGGAAATTG ATCGTGAATT GCAGAAGAAG 
AAAGAGGAGC TGAATGCAGT GCGTAGGCAA GCTGAGGGCT TGTCTGAGGA TGGGGCCGCA 
ATGGCAGTGG AGCCAACTCA GATCCAGCTC AGCAAGCGCT GGCGGGAAAT TGAGAGCAAA 
TTTGCTCAGT TTCGAAGACT CAACTTTGCA CAAATTCACA CTGTCCATGA AGAGTCAGTG 
GTGGCGATGA CTGAAGACAT GCCTTTGGAA ATTTCTTATG TGCCTTCTAC TTACCTGACT 
GAGATCACTC ATGTCTCACA AGCCCTATCA GAAGTGGAAG AGCTTCTTAA TGCTCCCGAC 
CTTTGTGCTC AAGATTTTGA AGATCTCTTT AAACAAGAGG AATCCTTGAA GAACATAAAA 
GACAGCCTGC AACAAATCTC AGGTCGGATT GACATCATTC ACAATAAAAA GACAGCAGCA 
TTGCACAGTG CCACTCCTGC AGAAAGGGCA AAGCTCCAGG AAGCTCTCTC ACGGCTTGAT 
TTCCAATGGG AAAGAGTTAA CAATATGTAC AAGGACCGAC AAGGGAGATT TGACAGATCT 
GTGGAAAAAT GGCGGCGGTT TCATTATGAT ATGAAGATAC TTAATCAATG GCTAACAGAA 
GCTGAACAGT TTCTCAAAAA GACACAAATT CCTGAGAATT GGGAACATGC CAAATACAAA 
TGGTATCTTA AGGAACTCCA GGATGGCATT GGACAGCGGC AAAGTGTTGT CAGGGTATTG 
AATGCAACTG GGGAAGAAAT AATTCAACAG TCCTCAAAAA CAGATGCCAG TATTCTCCAA 
GAAAAACTGG GAAGCCTGAA TCTGCGGTGG CAGGAGGTCT GCAAACAGCT GGCAGAAAGA 
AAAAAGAGGC TAGAGGAACA GAAGAATATC TTGTCAGAAT TTCAAAGAGA TGTAAATGAA 
TTTGTTTTAT GGTTGGAAGA AGCGGATAAC GTTGCTAATA TTCCACTTGA ACCTGGAAAT 
GAGCAGCAGC TAAAAGAAAA ACTTGAACAA GTCAAGTTAC TGGCAGAAGA GTTGCCCCTG 
CGCCAGGGAA TTCTAAAACA ATTAAATGAA ACTGGAGGAA CAGTGCTTGT AAGTGCTCCC 
CTAAGCCCAG AAGAGCAAGA TAAACTTGAA AATAAGCTCA AGCAGACAAA TCTTCAGTGG 
ATAAAGGTTT CTAGAAATCT GCCTGAGAAG CAAGAAGAAA TTGAGGCACA CGTAAAAGAC 
CTTGGACAGC TGGAAGAGCA GTTAAATCAT CTGCTTCTAT GGCTGTCTCC TATTAGGAAT 
CAGTTGGAAA TTTACAATCA GCCAAATCAA ACAGGACCAT TTGACATCAA GGAAATTGAA 
GTAGCAGTTC AAGCTAAACA GCCGGATGTG GAAGGGATTT TGTCTAAAGG GCAGCATTTG 
TACAAGGAAA AACCAGCCAC TCAGCCAGCG AAGAGAAAGC TGGAAGATCT CAGCTCTGAT 
TGGAAGGTGG TAACTCAGTT GCTTCAAGAG CTGCGGGCAA AGCAGCCTGG CCCAGCTCCT 
GGACTGACCA CTGTCAGAGC CCCTCCCAGT CAGACTGTTA CTCTGGTGAC ACAACCCGCG 
GTTACCAAGG AAACTGCCAT CTCCAAACTA GAAATGCCAT CTTCATTGCT GTTGGAGGTA 
CCTGCACTGG CAGATTTCAA CCGAGCTTGG ACAGAACTTA CCGACTGGCT GTCTCTGCTT 
GATCGAGTTA TAAAATCACA GAGGGTGATG GTGGGTGATC TTGAAGACAT TAACGAGATG 
ATCATCAAGC AGAAGGCAAC GCTGCAGGAT TTGGAACAGA GGCGCCCCCA GTTGGAAGAA 
CTCATTACCG CTGCCCAGAA TTTGAAAAAC AAGACCAGCA ATCAAGAGGC TAGAACAATC 
ATTACTGATC GAATTGAAAG AATTCAGAGT CAGTGGGATG AAGTACAGGA ACATCTTCAG 
AACCGGAGGC AACAGTTGAA TGAAATGTTA AAGGATTCCA CACAATGGCT GGAAGCTAAA 
GAGGAGGCTG AGCAGGTGTT GGGGCAGGCC AGAGCCAAGC TTGAGTCATG GAAGGAGGCT 
CCCTACACAG TAGATGCAAT CCAAAAGAAA ATCACAGAAA CCAAGCAGTT GGCCAAAGAC 
CTCCGCCAGT GGCAGATAAA TGTAGATGTT GCAAATGATT TGGCACTGAA ACTTCTCCGA 
GATTATTCTG CAGATGATAC CAGAAAAGTA CACATGATAA CAGAGAACAT CAATGCCTCT 
TGGGCAAGCA TCCATAAAAG ATTGAGTGAG CGAGAGGCTG CTCTGGAAGA AACCCACAGA 
TTACTGCAAC AGTTCCCCTT GGACCTGGAG AAGTTCCTTG CCTGGCTTAC AGAAGCCGAA 
ACAACTGCCA ACGTCCTGCA GGATGCCACC CATAAGGAAA GGCTTCTAGA AGATTCCAAG 
GGAGTAAGAG AGCTGATGAA ACAATGGCAA GATCTCCAAG GAGAAATCGA AGCTCACACA 
GATATCTATC ACAACCTGGA CGAAAATGGC CAAAAAGTCC TGAGATCCCT GGAAGGTTCT 
GACGATGCAG CCTTGTTGCA AAGACGTTTG GATAACATGA ACTTCAAGTG GAGCGAACTT 
CGGAAAAAGT CTCTCAACAT TAGGTCTCAC TTGGAAGCCA GTTCTGACCA GTGGAAGCGT 
CTGCACCTTT CTCTTCAGGA ACTTCTGGTA TGGCTCCAGC TGAAAGATGA TGAGTTAAGC 
CGGCAGGCAC CCATTGGAGG AGACTTTCCA GCGGTGCAGA AGCAGAATGA TGTACACAGG 
GCCTTCAAGA GGGAATTGAA AACGAAAGAA CCTGTAATCA TGAGTACTCT TGAGACTGTA 
CGAATATTTC TGACAGAGCA GCCTTTAGAA GGACTAGAGA AACTCTACCA GGAGCCCAGA 
GAGCTGCCTC CTGAAGAGAG AGCCCAGAAT GTCACACGGC TCCTACGAAA GCAAGCTGAG 
GAGGTCAACA CTCAGTGGGA AAAACTGAAC GTGCACTCTG CAGACTGGCA GAGAAAAATA 
GACGAGGCCC TCGAAAGACT CCAGGAGCTT CAGGAAGCAA CAGATGAGCT GGATCTCAAA 
CTACGTCAGG CAGAGGTGAT CAAAGGATCC TGGCAGCCTG TGGGTGATCT CCTCATTGAC 
TCTCTCCAAG ATCACCTCGA AAAAGTCAAG GCGCTTCGAG GAGAAATTAC ACCTCTGAAA 
GAGAATGTCA GCTACGTCAA TGACCTTGCT CGCCAACTCA CTACGTTGGG CATTCAGCTG 
TCACCATATA ACCTCAACAC TCTGGAAGAC CTGAACACCA GATGGAAGCT TCTGCAGGTG 
GCCATTGAGG ACCGCATCAG GCAGCTGCAT GAAGCGCACA GGGACTTTGG ACCAGCCTCC 
CAGCACTTCC TTTCCACTTC TGTCCAGGGT CCCTGGGAGA GAGCCATCTC ACCAAACAAA 
GTGCCCTACT ATATCAACCA CGAGACCCAA ACAACTTGCT GGGACCATCC CAAAATGACA 
GAGCTCTACC AGTCTTTAGC TGACCTGAAT AATGTCAGGT TCTCAGCTTA CAGGACTGCC 
ATGAAACTCC GAAGACTGCA GAAGGCCCTT TGCTTGGATC TCTTGAGCCT ATCGGCTGCA 
TGCGATGCCT TGGACCAGCA CAACCTCAAG CAAAATGACC AGCCCATGGA TATCCTGCAG 
GTCATTAACT GTCTGACCAC TATTTATGAT CGCCTAGAGC AAGAGCACAA CAATCTGGTC 
AACGTCCCTC TCTGCGTGGA TATGTGTCTC AATTGGCTGC TGAATGTTTA TGACACGGGA 
CGAACGGGGA GGATCCGGGT CCTGTCTTTT AAAACTGGCA TCATTTCTCT GTGTAAAGCC 
CATTTGGAAG ACAAGTACAG ATACCTCTTC AAGCAAGTGG CAAGTTCGAC AGGATTTTGT 
GACCAGCGCA GGCTGGGCCT CCTCCTGCAT GACTCTATCC AGATCCCAAG ACAGTTGGGT 
GAAGTCGCAT CGTTCGGGGG CAGTAACATT GAGCCGAGTG TCAGGAGCTG CTTCCAGTTT 
GCTAATAATA AGCCTGAGAT CGAAGCGGCC CTCTTCCTAG ACTGGATGCG CCTGGAGCCC 
CAGTCCATGG TGTGGCTGCC TGTCCTGCAC CGAGTGGCTG CCGCGGAAAC TGCCAAGCAC 
CAGGCCAAGT GCAACATCTG CAAGGAGTGT CCCATCATCG GATTCAGGTA CAGGAGTCTA 
AAGCACTTTA ATTATGACAT CTGCCAAAGT TGCTTTTTTT CTGGTCGAGT TGCAAAAGGC 
CATAAAATGC ACTATCCCAT GGTGGAATAC TGCACTCCGA CTACATCGGG AGAAGATGTC 
CGTGACTTTG CCAAGGTACT AAAAAACAAA TTTCGAACCA AAAGGTATTT TGCGAAGCAT 
CCCCGAATGG GCTACCTGCC AGTGCAGACT GTCTTAGAGG GGGACAACAT GGAAACTCCT 
GTCACTCTGA TCAACTTCTG GCCGGTAGAT TCTGCGCCTG CCTCGTCCCC TCAGCTTTCA 
CACGATGATA CTCATTCACG CATTGAGCAT TATGCTAGCA GGCTAGCAGA AATGGAAAAC 
AGCAATGGAT CTTATCTAAA TGATAGCATC TCTCCTAATG AGAGCATAGA TGATGAACAT 
TTGTTAATCC AGCATTACTG CCAAAGTTTG AACCAGGACT CCCCCCTGAG CCAGCCTCGT 
AGTCCTGCCC AGATCTTGAT TTCCTTAGAG AGTGAGGAAA GAGGGGAGCT AGAGAGAATC 
CTAGCAGATC TTGAGGAAGA AAACAGAAAT CTGCAAGCAG AGTATGATCG TCTAAAGCAG 
CAGCATGAAC ACAAAGGCCT GTCCCCACTG CCATCCCCTC CTGAAATGAT GCCTACTTCT 
CCCCAAAGTC CCCGGGATGC TGAGCTCATC GCTGAGGCCA AGCTGCTGCG TCAACACAAA 
GGCCGCCTGG AAGCCAGGAT GCAAATCCTT GAAGACCATA ACAAACAACT GGAATCCCAG 
TTACACAGGC TCAGGCAGCT GCTGGAGCAA CCCCAGGCAG AGGCCAAGGT GAATGGTACA 
ACGGTGTCTT CTCCTTCTAC CTCTCTTCAG AGGTCAGATA GCAGTCAGCC TATGCTGCTC 
CGGGTGGTCG GCAGTCAGAC TTCAGAATCC ATGGGCGAGG AAGACCTGCT CAGCCCTCCC 
CAGGACACAA GCACAGGGTT AGAGGAAGTG ATGGAGCAGC TCAACCACTC CTTCCCTAGT 
TCCAGAGGAC ACAATGTAGG AAGCCTTTTC CACTTGGCAG ATGATTTGGG CAGAGCGATG 
GAGTCCTTAG TTTCAGTCAT GACAGATGAA GAAGGGGCAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013966818.1
CDS	244..11328
Translation	MSEVSSDEREDVQKKTFTKWINAQFSKFGKQHIENLFSDLQDGRRLLDLLEGLTGQKLPKEKGSTRVHALNNVNKALRVLQKNNVDLVNIGSTDIVDGNHKLTLGLIWNIILHWQVKNVMKNIMAGLQQTNSEKILLSWVRQSTRNYPQVNVINFTTSWSDGLALNALIHSHRPDLFDWNSVVCQQSATQRLEHAFNIAKYQLGIEKLLDPEDVATTYPDKKSILMYITSLFQVLPQQVSIEAIQEVEMLPRPSKVTREEHFQLHHQMHYSQQITVSLAQGYERAPSSPKPRFKSYAYTQAAYVTTSDPTRSPLPSQHLETPEDKSFGRSLTETEANLDSYQTALEEVLSWLLSAEDALQAQGEISNDVEEVKEQFHTHEGYMMDLTSHQGRVGNVLQLGSQLIGTGKLSEDEETEVQEQMNLLNSRWECLRVASMEKQSNLHKVLMDLQNQQLKELNDWLTKTEERTRKMEKEPLGPDIEDLKRQVQQHKVLQEDLEQEQVRVNSLTHMVVVVDESSGDHATAALEEQLKVLGDRWANICRWTEDRWVLLQDILLKWQRFTEEQCLFSAWLSEKEDAVNKIHTTGFKDQSEVLSNLQKLAVLKTDLEKKKQTMDKLCSLNQDLLSALKNTVVAHKMEAWLDNFAQRWDNLVQKLEKSSAQISQAVTTTQPSLTQTTVMETVTMVTTREHILVKHAQEELPPPPPQKKRQIIVDSEIRKRLDVDITELHSWITRSEAVLQSPEFAIYRKEGNFSDLKEKVNAIEREKAEKFRKLQDASRSAQALVEQMVNEGVNADSIKQASEQLNSRWIEFCQLLSERLNWLEYQNNIITFYNQLQQLEQMTTTAENWLKTQPTTTSEPTAIKSQLKICKDEINRLSALQPQIERLKIQSIALKEKGQGPMFLDADFVAFTNHFNQVFADVQAREKELQTIFDSLPPMRYQETMSTILTWIQQSETKLSIPQVTVTEYDIMEQRLGELQALQSSLQEQQNGLNYLSTTVKEMSKKAPLSDISRKYQSEFEEIEGRWKKLSSQLVEHCQKLEEQMAKLRKIQNHIKTLKKWITEVDVFLKEEWPALGDSEILKRQLKQCRLLVNDIQTIQPSLNSVNEGAQKMKNEAEPEFAGRLETELRELNTQWDYMCRQVYARKEALKGGLDKTVSLQKDLSEMHEWMTQAEEEYLERDFEYKTPDELQTAVEEMKRAKEEAQQKEAKVKLLTESVNSVIAQAPPAAQEALKKELDTLTTNYQWLCTRLNGKCKTLEEVWACWHELLSYLEKANKWLSEVEVKLKTTENISGGAEEIAEVLDSLENLMQHSEDNPNQIRILAQTLTDGGVMDELINEELETFNSRWRELHEEAVRRQKLLEQSIQSAQEIEKSLHLIQESLSSIDKQLAAYIADKVDAAQMPQEAQKIQSDLTSHEISLEEMKKHNQGKETAQRVLSQIDVAQKKLQDVSMKFRLFQKPANFEQRLQESKMILDEVKMHLPALETKSVEQEVVQSQLNHCVNLYKSLSEVKSEVEMVIKTGRQIVQKKQTENPKELDERVTALKLHYNELGAKVTERKQQLEKCLKLSRKMRKEMNALTEWLAATDMELTKRSAVEGMPSNLDSEVAWGKATQKEIEKQKVHLKSVTEVGEALKTVLGKKEMLVEDKLSLLNSNWIAVTSRAEEWLNLLLEYQKHMENFDQNVDYITNWIIQADALLDESEKKKPQQKEDILKRLKAEMNDIRPKVDSTRDQAANLMANRGDHCRKVVEPKISELNHRFAAISHRIKTGKASIPLKELEQFNSDIQKLLEPLEAEIQQGVNLKEEDFNKDMSEDNEGTVKELLQRGDNLQQRITDERKREEIKIKQQLLQTKHNALKDLRSQRRKKALEISHQWYQYKRQADDLLKCLDDIEKKLASLPEPRDERKIKEIDRELQKKKEELNAVRRQAEGLSEDGAAMAVEPTQIQLSKRWREIESKFAQFRRLNFAQIHTVHEESVVAMTEDMPLEISYVPSTYLTEITHVSQALSEVEELLNAPDLCAQDFEDLFKQEESLKNIKDSLQQISGRIDIIHNKKTAALHSATPAERAKLQEALSRLDFQWERVNNMYKDRQGRFDRSVEKWRRFHYDMKILNQWLTEAEQFLKKTQIPENWEHAKYKWYLKELQDGIGQRQSVVRVLNATGEEIIQQSSKTDASILQEKLGSLNLRWQEVCKQLAERKKRLEEQKNILSEFQRDVNEFVLWLEEADNVANIPLEPGNEQQLKEKLEQVKLLAEELPLRQGILKQLNETGGTVLVSAPLSPEEQDKLENKLKQTNLQWIKVSRNLPEKQEEIEAHVKDLGQLEEQLNHLLLWLSPIRNQLEIYNQPNQTGPFDIKEIEVAVQAKQPDVEGILSKGQHLYKEKPATQPAKRKLEDLSSDWKVVTQLLQELRAKQPGPAPGLTTVRAPPSQTVTLVTQPAVTKETAISKLEMPSSLLLEVPALADFNRAWTELTDWLSLLDRVIKSQRVMVGDLEDINEMIIKQKATLQDLEQRRPQLEELITAAQNLKNKTSNQEARTIITDRIERIQSQWDEVQEHLQNRRQQLNEMLKDSTQWLEAKEEAEQVLGQARAKLESWKEAPYTVDAIQKKITETKQLAKDLRQWQINVDVANDLALKLLRDYSADDTRKVHMITENINASWASIHKRLSEREAALEETHRLLQQFPLDLEKFLAWLTEAETTANVLQDATHKERLLEDSKGVRELMKQWQDLQGEIEAHTDIYHNLDENGQKVLRSLEGSDDAALLQRRLDNMNFKWSELRKKSLNIRSHLEASSDQWKRLHLSLQELLVWLQLKDDELSRQAPIGGDFPAVQKQNDVHRAFKRELKTKEPVIMSTLETVRIFLTEQPLEGLEKLYQEPRELPPEERAQNVTRLLRKQAEEVNTQWEKLNVHSADWQRKIDEALERLQELQEATDELDLKLRQAEVIKGSWQPVGDLLIDSLQDHLEKVKALRGEITPLKENVSYVNDLARQLTTLGIQLSPYNLNTLEDLNTRWKLLQVAIEDRIRQLHEAHRDFGPASQHFLSTSVQGPWERAISPNKVPYYINHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQVINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPVTLINFWPVDSAPASSPQLSHDDTHSRIEHYASRLAEMENSNGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKQQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDTSTGLEEVMEQLNHSFPSSRGHNVGSLFHLADDLGRAMESLVSVMTDEEGAE

Target ORF information:

RefSeq Version	XM_014111343.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris dystrophin (DMD), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111343.1

ORF Insert Sequence:

ATGTCTGAGG TCTCATCTGA TGAAAGAGAA GATGTTCAAA AGAAAACATT CACAAAATGG 
ATAAATGCAC AGTTTTCTAA GTTTGGGAAG CAGCACATAG AGAACCTCTT CAGTGACCTA 
CAGGATGGGA GACGCCTCCT AGACCTTTTG GAAGGCCTGA CAGGGCAAAA ACTGCCAAAA 
GAAAAAGGAT CCACAAGAGT TCATGCCCTG AACAATGTCA ACAAGGCACT GCGCGTCTTG 
CAGAAAAATA ATGTTGATTT AGTGAACATT GGAAGTACTG ACATAGTAGA TGGAAATCAC 
AAACTGACTC TTGGTTTGAT TTGGAATATA ATCCTCCACT GGCAGGTCAA AAATGTAATG 
AAAAATATCA TGGCTGGATT GCAACAAACC AACAGTGAAA AGATTCTCCT GAGCTGGGTC 
CGACAATCAA CTCGTAATTA TCCACAGGTT AATGTCATTA ACTTCACCAC CAGCTGGTCT 
GATGGCCTGG CTTTGAACGC TCTCATCCAC AGTCATAGGC CAGACCTGTT TGATTGGAAT 
AGTGTGGTTT GCCAGCAGTC AGCCACACAA CGCCTGGAAC ATGCATTCAA CATTGCCAAA 
TATCAATTAG GCATAGAGAA ACTGCTTGAT CCTGAAGATG TTGCCACCAC TTATCCAGAT 
AAGAAGTCCA TCTTAATGTA TATCACATCA CTCTTCCAAG TTTTGCCTCA ACAAGTGAGC 
ATTGAAGCCA TCCAGGAAGT GGAAATGTTG CCAAGGCCAT CAAAAGTTAC TAGAGAAGAA 
CATTTTCAAT TACATCATCA AATGCACTAT TCTCAACAGA TCACAGTCAG TCTAGCACAG 
GGCTATGAAC GAGCCCCTTC CTCTCCTAAG CCTCGGTTCA AGAGCTATGC CTACACACAG 
GCTGCTTATG TCACCACTTC TGACCCCACA CGGAGCCCAC TTCCTTCACA GCATTTGGAA 
ACTCCTGAAG ACAAGTCATT TGGCCGGTCA TTGACAGAGA CCGAAGCAAA CCTGGACAGT 
TATCAAACAG CTTTGGAAGA AGTACTCTCG TGGCTTCTTT CAGCTGAGGA TGCACTGCAA 
GCCCAAGGAG AGATTTCTAA TGATGTCGAA GAAGTGAAAG AACAATTTCA TACTCATGAG 
GGATATATGA TGGACTTGAC ATCCCATCAG GGACGGGTCG GTAATGTTCT CCAACTGGGA 
AGTCAACTGA TTGGAACAGG GAAATTATCA GAAGATGAAG AAACCGAAGT GCAGGAACAA 
ATGAATCTCC TCAATTCAAG ATGGGAATGC CTCAGGGTAG CTAGCATGGA AAAACAAAGC 
AATTTACATA AAGTTCTAAT GGATCTCCAG AATCAGCAAC TGAAAGAGTT AAATGACTGG 
CTAACCAAAA CAGAAGAGAG AACAAGGAAA ATGGAGAAGG AGCCCCTTGG ACCTGATATT 
GAAGACCTAA AACGCCAAGT ACAACAACAT AAGGTGCTTC AAGAAGACTT AGAACAGGAA 
CAAGTCAGGG TCAATTCCCT CACTCATATG GTGGTGGTAG TCGATGAATC TAGTGGAGAC 
CATGCAACTG CTGCTTTGGA AGAACAACTT AAGGTACTGG GAGATCGATG GGCAAACATC 
TGTAGGTGGA CAGAAGATCG CTGGGTTCTT TTACAAGACA TCCTCCTAAA ATGGCAGCGT 
TTTACTGAAG AACAGTGCCT TTTCAGTGCA TGGCTTTCGG AGAAGGAAGA TGCAGTGAAC 
AAGATTCACA CAACTGGCTT TAAGGATCAA AGTGAAGTGT TATCAAATCT TCAGAAACTG 
GCTGTCTTAA AAACAGATCT GGAAAAGAAG AAGCAAACCA TGGACAAACT CTGCTCACTC 
AACCAAGACC TTCTTTCAGC GCTGAAAAAC ACAGTGGTAG CCCACAAGAT GGAAGCATGG 
CTGGACAACT TTGCCCAGCG CTGGGATAAT TTAGTCCAGA AACTTGAAAA AAGTTCAGCA 
CAGATTTCAC AGGCTGTCAC CACCACTCAG CCATCACTAA CACAGACAAC TGTAATGGAA 
ACAGTAACTA TGGTGACCAC GAGGGAACAC ATCTTGGTAA AGCATGCCCA AGAGGAACTG 
CCACCACCAC CCCCTCAGAA GAAGAGGCAG ATTATCGTGG ATTCTGAAAT TAGGAAAAGG 
TTGGATGTCG ATATAACTGA ACTTCACAGT TGGATTACTC GTTCAGAAGC TGTGTTGCAG 
AGTCCTGAAT TTGCAATCTA TCGGAAGGAA GGCAACTTCT CAGACCTTAA AGAAAAAGTC 
AATGCCATAG AGCGAGAAAA AGCCGAGAAG TTCAGAAAAC TGCAAGATGC CAGCAGATCA 
GCTCAGGCCC TGGTGGAACA GATGGTGAAT GAGGGTGTTA ATGCTGACAG CATCAAACAA 
GCCTCCGAAC AACTGAACAG CCGGTGGATA GAGTTCTGCC AATTGCTAAG CGAGAGACTT 
AACTGGCTGG AGTATCAGAA CAACATCATC ACTTTCTATA ATCAGCTACA ACAATTGGAG 
CAGATGACAA CTACTGCTGA AAACTGGTTG AAAACCCAGC CTACCACCAC ATCAGAGCCA 
ACAGCAATTA AAAGCCAGTT AAAAATTTGT AAGGATGAAA TCAACCGACT GTCAGCTCTT 
CAGCCTCAAA TCGAGCGATT AAAAATTCAA AGCATAGCCC TGAAAGAGAA AGGACAAGGG 
CCAATGTTCC TGGATGCAGA CTTTGTGGCC TTTACAAATC ATTTTAACCA AGTCTTTGCT 
GATGTGCAGG CAAGAGAAAA AGAGCTACAA ACAATTTTTG ACAGTTTGCC ACCCATGCGC 
TATCAGGAGA CTATGAGTAC CATCCTGACA TGGATCCAGC AGTCAGAAAC CAAACTCTCT 
ATACCTCAGG TTACTGTCAC TGAATATGAC ATCATGGAAC AGAGACTCGG AGAGCTACAG 
GCTTTACAAA GCTCTCTGCA AGAGCAACAA AATGGCCTAA ACTATCTCAG CACCACTGTG 
AAAGAGATGT CAAAGAAAGC ACCACTGTCT GATATTAGTC GGAAATATCA ATCAGAATTT 
GAAGAGATTG AGGGACGTTG GAAGAAGCTG TCTTCCCAGC TGGTTGAACA TTGTCAAAAG 
TTGGAGGAGC AAATGGCTAA ACTTCGAAAA ATTCAGAATC ACATAAAAAC TCTGAAGAAA 
TGGATCACTG AAGTCGATGT TTTCCTGAAG GAGGAATGGC CTGCCCTTGG GGATTCAGAA 
ATTCTGAAAA GACAGCTGAA ACAGTGCAGG CTTTTAGTCA ATGACATTCA GACCATCCAG 
CCTAGTCTCA ACAGTGTCAA TGAAGGGGCT CAGAAGATGA AGAATGAAGC AGAACCAGAG 
TTTGCTGGCA GACTTGAGAC AGAGCTCCGA GAACTTAACA CCCAGTGGGA TTACATGTGC 
CGCCAGGTCT ATGCCAGGAA GGAAGCCTTA AAAGGAGGTT TGGATAAAAC TGTAAGTCTT 
CAGAAAGATC TGTCAGAGAT GCATGAGTGG ATGACACAAG CTGAAGAAGA ATACCTAGAG 
AGAGATTTCG AATACAAGAC CCCTGATGAA TTACAGACAG CAGTTGAAGA GATGAAGAGA 
GCTAAAGAAG AGGCCCAGCA AAAAGAAGCA AAAGTGAAAC TCCTAACCGA GTCCGTCAAT 
AGTGTCATAG CTCAGGCTCC ACCTGCAGCA CAAGAGGCCT TAAAAAAGGA ACTTGACACT 
CTCACCACCA ACTACCAGTG GCTCTGCACC AGGCTCAATG GCAAATGCAA GACCTTGGAA 
GAAGTTTGGG CGTGCTGGCA TGAGTTATTG TCCTACTTGG AGAAGGCAAA CAAGTGGCTA 
AGTGAAGTAG AAGTCAAGCT TAAAACCACT GAAAATATTT CTGGGGGAGC TGAGGAAATC 
GCCGAGGTGC TTGATTCGCT TGAAAATTTG ATGCAACATT CAGAGGATAA CCCGAATCAG 
ATTCGCATAT TGGCACAGAC CTTGACAGAT GGTGGAGTCA TGGATGAACT GATCAATGAG 
GAGCTTGAGA CATTTAATTC TCGTTGGAGA GAACTCCATG AAGAGGCTGT GAGGAGGCAA 
AAGTTGCTTG AGCAGAGTAT CCAGTCGGCC CAGGAGATAG AAAAATCCTT GCACTTAATT 
CAGGAGTCCC TCTCTTCCAT TGACAAGCAG TTGGCAGCTT ATATTGCTGA CAAAGTGGAT 
GCAGCTCAGA TGCCTCAGGA AGCCCAGAAA ATCCAATCAG ATTTGACAAG TCATGAGATC 
AGTTTAGAAG AAATGAAGAA ACATAACCAG GGAAAGGAGA CTGCCCAAAG GGTACTATCC 
CAAATTGATG TGGCACAGAA AAAATTGCAG GATGTTTCCA TGAAGTTTCG ATTATTCCAG 
AAGCCAGCCA ATTTTGAGCA GCGCCTACAA GAAAGTAAAA TGATTTTAGA TGAAGTGAAG 
ATGCATTTAC CTGCGTTGGA AACAAAGAGT GTGGAACAGG AAGTAGTACA GTCACAGTTA 
AATCATTGTG TGAACTTGTA TAAAAGTCTG AGTGAAGTGA AGTCTGAAGT GGAAATGGTA 
ATAAAAACTG GACGTCAGAT TGTACAGAAG AAGCAGACGG AAAACCCGAA AGAGCTTGAT 
GAAAGAGTTA CAGCTTTGAA ATTGCATTAT AATGAGCTGG GAGCAAAGGT GACAGAAAGA 
AAGCAACAGT TGGAAAAATG CTTGAAATTG TCCCGTAAGA TGCGAAAGGA AATGAATGCC 
CTGACAGAAT GGCTGGCAGC GACAGATATG GAACTGACGA AGAGATCGGC AGTTGAAGGA 
ATGCCTAGTA ATTTGGATTC TGAAGTTGCC TGGGGAAAGG CTACTCAGAA AGAGATTGAG 
AAACAGAAGG TTCACCTAAA GAGTGTCACA GAGGTAGGAG AGGCCTTGAA AACGGTTTTG 
GGCAAGAAGG AAATGTTGGT GGAAGATAAA CTGAGTCTTC TGAATAGTAA CTGGATAGCC 
GTCACTTCCC GAGCAGAAGA GTGGTTAAAC CTTTTATTGG AATACCAGAA ACACATGGAA 
AACTTTGACC AGAATGTGGA TTACATCACA AACTGGATCA TTCAGGCTGA TGCACTTTTG 
GATGAATCTG AGAAAAAGAA ACCTCAGCAA AAAGAAGACA TACTTAAGCG TTTAAAGGCT 
GAAATGAATG ACATACGTCC AAAGGTGGAT TCTACACGTG ACCAAGCAGC AAACCTGATG 
GCAAACCGCG GCGACCACTG CAGGAAAGTA GTAGAGCCCA AAATCTCAGA GCTCAACCAT 
CGATTTGCAG CCATTTCTCA CAGAATTAAG ACTGGAAAGG CCTCCATTCC TTTGAAGGAA 
TTGGAGCAGT TTAACTCAGA TATACAAAAA TTGCTTGAAC CACTGGAGGC TGAAATTCAG 
CAGGGGGTGA ATCTGAAAGA GGAAGACTTC AATAAAGATA TGAGTGAAGA CAATGAGGGT 
ACTGTAAAAG AATTGTTGCA AAGAGGAGAC AACTTACAAC AAAGAATCAC AGATGAGAGA 
AAGCGAGAGG AAATAAAGAT AAAACAACAG CTGTTACAGA CAAAACATAA TGCTCTCAAG 
GATTTGAGGT CTCAAAGAAG AAAAAAGGCT CTAGAAATTT CTCACCAGTG GTATCAGTAC 
AAGAGGCAGG CTGATGATCT CCTGAAATGC TTGGATGACA TTGAAAAAAA ATTAGCCAGC 
CTACCTGAAC CCAGAGATGA AAGGAAAATA AAGGAAATTG ATCGTGAATT GCAGAAGAAG 
AAAGAGGAGC TGAATGCAGT GCGTAGGCAA GCTGAGGGCT TGTCTGAGGA TGGGGCCGCA 
ATGGCAGTGG AGCCAACTCA GATCCAGCTC AGCAAGCGCT GGCGGGAAAT TGAGAGCAAA 
TTTGCTCAGT TTCGAAGACT CAACTTTGCA CAAATTCACA CTGTCCATGA AGAGTCAGTG 
GTGGCGATGA CTGAAGACAT GCCTTTGGAA ATTTCTTATG TGCCTTCTAC TTACCTGACT 
GAGATCACTC ATGTCTCACA AGCCCTATCA GAAGTGGAAG AGCTTCTTAA TGCTCCCGAC 
CTTTGTGCTC AAGATTTTGA AGATCTCTTT AAACAAGAGG AATCCTTGAA GAACATAAAA 
GACAGCCTGC AACAAATCTC AGGTCGGATT GACATCATTC ACAATAAAAA GACAGCAGCA 
TTGCACAGTG CCACTCCTGC AGAAAGGGCA AAGCTCCAGG AAGCTCTCTC ACGGCTTGAT 
TTCCAATGGG AAAGAGTTAA CAATATGTAC AAGGACCGAC AAGGGAGATT TGACAGATCT 
GTGGAAAAAT GGCGGCGGTT TCATTATGAT ATGAAGATAC TTAATCAATG GCTAACAGAA 
GCTGAACAGT TTCTCAAAAA GACACAAATT CCTGAGAATT GGGAACATGC CAAATACAAA 
TGGTATCTTA AGGAACTCCA GGATGGCATT GGACAGCGGC AAAGTGTTGT CAGGGTATTG 
AATGCAACTG GGGAAGAAAT AATTCAACAG TCCTCAAAAA CAGATGCCAG TATTCTCCAA 
GAAAAACTGG GAAGCCTGAA TCTGCGGTGG CAGGAGGTCT GCAAACAGCT GGCAGAAAGA 
AAAAAGAGGC TAGAGGAACA GAAGAATATC TTGTCAGAAT TTCAAAGAGA TGTAAATGAA 
TTTGTTTTAT GGTTGGAAGA AGCGGATAAC GTTGCTAATA TTCCACTTGA ACCTGGAAAT 
GAGCAGCAGC TAAAAGAAAA ACTTGAACAA GTCAAGTTAC TGGCAGAAGA GTTGCCCCTG 
CGCCAGGGAA TTCTAAAACA ATTAAATGAA ACTGGAGGAA CAGTGCTTGT AAGTGCTCCC 
CTAAGCCCAG AAGAGCAAGA TAAACTTGAA AATAAGCTCA AGCAGACAAA TCTTCAGTGG 
ATAAAGGTTT CTAGAAATCT GCCTGAGAAG CAAGAAGAAA TTGAGGCACA CGTAAAAGAC 
CTTGGACAGC TGGAAGAGCA GTTAAATCAT CTGCTTCTAT GGCTGTCTCC TATTAGGAAT 
CAGTTGGAAA TTTACAATCA GCCAAATCAA ACAGGACCAT TTGACATCAA GGAAATTGAA 
GTAGCAGTTC AAGCTAAACA GCCGGATGTG GAAGGGATTT TGTCTAAAGG GCAGCATTTG 
TACAAGGAAA AACCAGCCAC TCAGCCAGCG AAGAGAAAGC TGGAAGATCT CAGCTCTGAT 
TGGAAGGTGG TAACTCAGTT GCTTCAAGAG CTGCGGGCAA AGCAGCCTGG CCCAGCTCCT 
GGACTGACCA CTGTCAGAGC CCCTCCCAGT CAGACTGTTA CTCTGGTGAC ACAACCCGCG 
GTTACCAAGG AAACTGCCAT CTCCAAACTA GAAATGCCAT CTTCATTGCT GTTGGAGGTA 
CCTGCACTGG CAGATTTCAA CCGAGCTTGG ACAGAACTTA CCGACTGGCT GTCTCTGCTT 
GATCGAGTTA TAAAATCACA GAGGGTGATG GTGGGTGATC TTGAAGACAT TAACGAGATG 
ATCATCAAGC AGAAGGCAAC GCTGCAGGAT TTGGAACAGA GGCGCCCCCA GTTGGAAGAA 
CTCATTACCG CTGCCCAGAA TTTGAAAAAC AAGACCAGCA ATCAAGAGGC TAGAACAATC 
ATTACTGATC GAATTGAAAG AATTCAGAGT CAGTGGGATG AAGTACAGGA ACATCTTCAG 
AACCGGAGGC AACAGTTGAA TGAAATGTTA AAGGATTCCA CACAATGGCT GGAAGCTAAA 
GAGGAGGCTG AGCAGGTGTT GGGGCAGGCC AGAGCCAAGC TTGAGTCATG GAAGGAGGCT 
CCCTACACAG TAGATGCAAT CCAAAAGAAA ATCACAGAAA CCAAGCAGTT GGCCAAAGAC 
CTCCGCCAGT GGCAGATAAA TGTAGATGTT GCAAATGATT TGGCACTGAA ACTTCTCCGA 
GATTATTCTG CAGATGATAC CAGAAAAGTA CACATGATAA CAGAGAACAT CAATGCCTCT 
TGGGCAAGCA TCCATAAAAG ATTGAGTGAG CGAGAGGCTG CTCTGGAAGA AACCCACAGA 
TTACTGCAAC AGTTCCCCTT GGACCTGGAG AAGTTCCTTG CCTGGCTTAC AGAAGCCGAA 
ACAACTGCCA ACGTCCTGCA GGATGCCACC CATAAGGAAA GGCTTCTAGA AGATTCCAAG 
GGAGTAAGAG AGCTGATGAA ACAATGGCAA GATCTCCAAG GAGAAATCGA AGCTCACACA 
GATATCTATC ACAACCTGGA CGAAAATGGC CAAAAAGTCC TGAGATCCCT GGAAGGTTCT 
GACGATGCAG CCTTGTTGCA AAGACGTTTG GATAACATGA ACTTCAAGTG GAGCGAACTT 
CGGAAAAAGT CTCTCAACAT TAGGTCTCAC TTGGAAGCCA GTTCTGACCA GTGGAAGCGT 
CTGCACCTTT CTCTTCAGGA ACTTCTGGTA TGGCTCCAGC TGAAAGATGA TGAGTTAAGC 
CGGCAGGCAC CCATTGGAGG AGACTTTCCA GCGGTGCAGA AGCAGAATGA TGTACACAGG 
GCCTTCAAGA GGGAATTGAA AACGAAAGAA CCTGTAATCA TGAGTACTCT TGAGACTGTA 
CGAATATTTC TGACAGAGCA GCCTTTAGAA GGACTAGAGA AACTCTACCA GGAGCCCAGA 
GAGCTGCCTC CTGAAGAGAG AGCCCAGAAT GTCACACGGC TCCTACGAAA GCAAGCTGAG 
GAGGTCAACA CTCAGTGGGA AAAACTGAAC GTGCACTCTG CAGACTGGCA GAGAAAAATA 
GACGAGGCCC TCGAAAGACT CCAGGAGCTT CAGGAAGCAA CAGATGAGCT GGATCTCAAA 
CTACGTCAGG CAGAGGTGAT CAAAGGATCC TGGCAGCCTG TGGGTGATCT CCTCATTGAC 
TCTCTCCAAG ATCACCTCGA AAAAGTCAAG GCGCTTCGAG GAGAAATTAC ACCTCTGAAA 
GAGAATGTCA GCTACGTCAA TGACCTTGCT CGCCAACTCA CTACGTTGGG CATTCAGCTG 
TCACCATATA ACCTCAACAC TCTGGAAGAC CTGAACACCA GATGGAAGCT TCTGCAGGTG 
GCCATTGAGG ACCGCATCAG GCAGCTGCAT GAAGCGCACA GGGACTTTGG ACCAGCCTCC 
CAGCACTTCC TTTCCACTTC TGTCCAGGGT CCCTGGGAGA GAGCCATCTC ACCAAACAAA 
GTGCCCTACT ATATCAACCA CGAGACCCAA ACAACTTGCT GGGACCATCC CAAAATGACA 
GAGCTCTACC AGTCTTTAGC TGACCTGAAT AATGTCAGGT TCTCAGCTTA CAGGACTGCC 
ATGAAACTCC GAAGACTGCA GAAGGCCCTT TGCTTGGATC TCTTGAGCCT ATCGGCTGCA 
TGCGATGCCT TGGACCAGCA CAACCTCAAG CAAAATGACC AGCCCATGGA TATCCTGCAG 
GTCATTAACT GTCTGACCAC TATTTATGAT CGCCTAGAGC AAGAGCACAA CAATCTGGTC 
AACGTCCCTC TCTGCGTGGA TATGTGTCTC AATTGGCTGC TGAATGTTTA TGACACGGGA 
CGAACGGGGA GGATCCGGGT CCTGTCTTTT AAAACTGGCA TCATTTCTCT GTGTAAAGCC 
CATTTGGAAG ACAAGTACAG ATACCTCTTC AAGCAAGTGG CAAGTTCGAC AGGATTTTGT 
GACCAGCGCA GGCTGGGCCT CCTCCTGCAT GACTCTATCC AGATCCCAAG ACAGTTGGGT 
GAAGTCGCAT CGTTCGGGGG CAGTAACATT GAGCCGAGTG TCAGGAGCTG CTTCCAGTTT 
GCTAATAATA AGCCTGAGAT CGAAGCGGCC CTCTTCCTAG ACTGGATGCG CCTGGAGCCC 
CAGTCCATGG TGTGGCTGCC TGTCCTGCAC CGAGTGGCTG CCGCGGAAAC TGCCAAGCAC 
CAGGCCAAGT GCAACATCTG CAAGGAGTGT CCCATCATCG GATTCAGGTA CAGGAGTCTA 
AAGCACTTTA ATTATGACAT CTGCCAAAGT TGCTTTTTTT CTGGTCGAGT TGCAAAAGGC 
CATAAAATGC ACTATCCCAT GGTGGAATAC TGCACTCCGA CTACATCGGG AGAAGATGTC 
CGTGACTTTG CCAAGGTACT AAAAAACAAA TTTCGAACCA AAAGGTATTT TGCGAAGCAT 
CCCCGAATGG GCTACCTGCC AGTGCAGACT GTCTTAGAGG GGGACAACAT GGAAACTCCT 
GTCACTCTGA TCAACTTCTG GCCGGTAGAT TCTGCGCCTG CCTCGTCCCC TCAGCTTTCA 
CACGATGATA CTCATTCACG CATTGAGCAT TATGCTAGCA GGCTAGCAGA AATGGAAAAC 
AGCAATGGAT CTTATCTAAA TGATAGCATC TCTCCTAATG AGAGCATAGA TGATGAACAT 
TTGTTAATCC AGCATTACTG CCAAAGTTTG AACCAGGACT CCCCCCTGAG CCAGCCTCGT 
AGTCCTGCCC AGATCTTGAT TTCCTTAGAG AGTGAGGAAA GAGGGGAGCT AGAGAGAATC 
CTAGCAGATC TTGAGGAAGA AAACAGAAAT CTGCAAGCAG AGTATGATCG TCTAAAGCAG 
CAGCATGAAC ACAAAGGCCT GTCCCCACTG CCATCCCCTC CTGAAATGAT GCCTACTTCT 
CCCCAAAGTC CCCGGGATGC TGAGCTCATC GCTGAGGCCA AGCTGCTGCG TCAACACAAA 
GGCCGCCTGG AAGCCAGGAT GCAAATCCTT GAAGACCATA ACAAACAACT GGAATCCCAG 
TTACACAGGC TCAGGCAGCT GCTGGAGCAA CCCCAGGCAG AGGCCAAGGT GAATGGTACA 
ACGGTGTCTT CTCCTTCTAC CTCTCTTCAG AGGTCAGATA GCAGTCAGCC TATGCTGCTC 
CGGGTGGTCG GCAGTCAGAC TTCAGAATCC ATGGGCGAGG AAGACCTGCT CAGCCCTCCC 
CAGGACACAA GCACAGGGTT AGAGGAAGTG ATGGAGCAGC TCAACCACTC CTTCCCTAGT 
TCCAGAGGAC ACAATGTAGG AAGCCTTTTC CACTTGGCAG ATGATTTGGG CAGAGCGATG 
GAGTCCTTAG TTTCAGTCAT GACAGATGAA GAAGGGGCAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMD ( XM_014111344.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013966819.1 Canis lupus familiaris dystrophin (DMD), transcript variant X4, mRNA.

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CloneID	OCe45415
Clone ID Related Accession (Same CDS sequence)	XM_014111344.1 , XM_014111344.2
Accession Version	XM_014111344.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	dystrophin isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGATG AAAGAGAAGA TGTTCAAAAG AAAACATTCA CAAAATGGAT AAATGCACAG 
TTTTCTAAGT TTGGGAAGCA GCACATAGAG AACCTCTTCA GTGACCTACA GGATGGGAGA 
CGCCTCCTAG ACCTTTTGGA AGGCCTGACA GGGCAAAAAC TGCCAAAAGA AAAAGGATCC 
ACAAGAGTTC ATGCCCTGAA CAATGTCAAC AAGGCACTGC GCGTCTTGCA GAAAAATAAT 
GTTGATTTAG TGAACATTGG AAGTACTGAC ATAGTAGATG GAAATCACAA ACTGACTCTT 
GGTTTGATTT GGAATATAAT CCTCCACTGG CAGGTCAAAA ATGTAATGAA AAATATCATG 
GCTGGATTGC AACAAACCAA CAGTGAAAAG ATTCTCCTGA GCTGGGTCCG ACAATCAACT 
CGTAATTATC CACAGGTTAA TGTCATTAAC TTCACCACCA GCTGGTCTGA TGGCCTGGCT 
TTGAACGCTC TCATCCACAG TCATAGGCCA GACCTGTTTG ATTGGAATAG TGTGGTTTGC 
CAGCAGTCAG CCACACAACG CCTGGAACAT GCATTCAACA TTGCCAAATA TCAATTAGGC 
ATAGAGAAAC TGCTTGATCC TGAAGATGTT GCCACCACTT ATCCAGATAA GAAGTCCATC 
TTAATGTATA TCACATCACT CTTCCAAGTT TTGCCTCAAC AAGTGAGCAT TGAAGCCATC 
CAGGAAGTGG AAATGTTGCC AAGGCCATCA AAAGTTACTA GAGAAGAACA TTTTCAATTA 
CATCATCAAA TGCACTATTC TCAACAGATC ACAGTCAGTC TAGCACAGGG CTATGAACGA 
GCCCCTTCCT CTCCTAAGCC TCGGTTCAAG AGCTATGCCT ACACACAGGC TGCTTATGTC 
ACCACTTCTG ACCCCACACG GAGCCCACTT CCTTCACAGC ATTTGGAAAC TCCTGAAGAC 
AAGTCATTTG GCCGGTCATT GACAGAGACC GAAGCAAACC TGGACAGTTA TCAAACAGCT 
TTGGAAGAAG TACTCTCGTG GCTTCTTTCA GCTGAGGATG CACTGCAAGC CCAAGGAGAG 
ATTTCTAATG ATGTCGAAGA AGTGAAAGAA CAATTTCATA CTCATGAGGG ATATATGATG 
GACTTGACAT CCCATCAGGG ACGGGTCGGT AATGTTCTCC AACTGGGAAG TCAACTGATT 
GGAACAGGGA AATTATCAGA AGATGAAGAA ACCGAAGTGC AGGAACAAAT GAATCTCCTC 
AATTCAAGAT GGGAATGCCT CAGGGTAGCT AGCATGGAAA AACAAAGCAA TTTACATAAA 
GTTCTAATGG ATCTCCAGAA TCAGCAACTG AAAGAGTTAA ATGACTGGCT AACCAAAACA 
GAAGAGAGAA CAAGGAAAAT GGAGAAGGAG CCCCTTGGAC CTGATATTGA AGACCTAAAA 
CGCCAAGTAC AACAACATAA GGTGCTTCAA GAAGACTTAG AACAGGAACA AGTCAGGGTC 
AATTCCCTCA CTCATATGGT GGTGGTAGTC GATGAATCTA GTGGAGACCA TGCAACTGCT 
GCTTTGGAAG AACAACTTAA GGTACTGGGA GATCGATGGG CAAACATCTG TAGGTGGACA 
GAAGATCGCT GGGTTCTTTT ACAAGACATC CTCCTAAAAT GGCAGCGTTT TACTGAAGAA 
CAGTGCCTTT TCAGTGCATG GCTTTCGGAG AAGGAAGATG CAGTGAACAA GATTCACACA 
ACTGGCTTTA AGGATCAAAG TGAAGTGTTA TCAAATCTTC AGAAACTGGC TGTCTTAAAA 
ACAGATCTGG AAAAGAAGAA GCAAACCATG GACAAACTCT GCTCACTCAA CCAAGACCTT 
CTTTCAGCGC TGAAAAACAC AGTGGTAGCC CACAAGATGG AAGCATGGCT GGACAACTTT 
GCCCAGCGCT GGGATAATTT AGTCCAGAAA CTTGAAAAAA GTTCAGCACA GATTTCACAG 
GCTGTCACCA CCACTCAGCC ATCACTAACA CAGACAACTG TAATGGAAAC AGTAACTATG 
GTGACCACGA GGGAACACAT CTTGGTAAAG CATGCCCAAG AGGAACTGCC ACCACCACCC 
CCTCAGAAGA AGAGGCAGAT TATCGTGGAT TCTGAAATTA GGAAAAGGTT GGATGTCGAT 
ATAACTGAAC TTCACAGTTG GATTACTCGT TCAGAAGCTG TGTTGCAGAG TCCTGAATTT 
GCAATCTATC GGAAGGAAGG CAACTTCTCA GACCTTAAAG AAAAAGTCAA TGCCATAGAG 
CGAGAAAAAG CCGAGAAGTT CAGAAAACTG CAAGATGCCA GCAGATCAGC TCAGGCCCTG 
GTGGAACAGA TGGTGAATGA GGGTGTTAAT GCTGACAGCA TCAAACAAGC CTCCGAACAA 
CTGAACAGCC GGTGGATAGA GTTCTGCCAA TTGCTAAGCG AGAGACTTAA CTGGCTGGAG 
TATCAGAACA ACATCATCAC TTTCTATAAT CAGCTACAAC AATTGGAGCA GATGACAACT 
ACTGCTGAAA ACTGGTTGAA AACCCAGCCT ACCACCACAT CAGAGCCAAC AGCAATTAAA 
AGCCAGTTAA AAATTTGTAA GGATGAAATC AACCGACTGT CAGCTCTTCA GCCTCAAATC 
GAGCGATTAA AAATTCAAAG CATAGCCCTG AAAGAGAAAG GACAAGGGCC AATGTTCCTG 
GATGCAGACT TTGTGGCCTT TACAAATCAT TTTAACCAAG TCTTTGCTGA TGTGCAGGCA 
AGAGAAAAAG AGCTACAAAC AATTTTTGAC AGTTTGCCAC CCATGCGCTA TCAGGAGACT 
ATGAGTACCA TCCTGACATG GATCCAGCAG TCAGAAACCA AACTCTCTAT ACCTCAGGTT 
ACTGTCACTG AATATGACAT CATGGAACAG AGACTCGGAG AGCTACAGGC TTTACAAAGC 
TCTCTGCAAG AGCAACAAAA TGGCCTAAAC TATCTCAGCA CCACTGTGAA AGAGATGTCA 
AAGAAAGCAC CACTGTCTGA TATTAGTCGG AAATATCAAT CAGAATTTGA AGAGATTGAG 
GGACGTTGGA AGAAGCTGTC TTCCCAGCTG GTTGAACATT GTCAAAAGTT GGAGGAGCAA 
ATGGCTAAAC TTCGAAAAAT TCAGAATCAC ATAAAAACTC TGAAGAAATG GATCACTGAA 
GTCGATGTTT TCCTGAAGGA GGAATGGCCT GCCCTTGGGG ATTCAGAAAT TCTGAAAAGA 
CAGCTGAAAC AGTGCAGGCT TTTAGTCAAT GACATTCAGA CCATCCAGCC TAGTCTCAAC 
AGTGTCAATG AAGGGGCTCA GAAGATGAAG AATGAAGCAG AACCAGAGTT TGCTGGCAGA 
CTTGAGACAG AGCTCCGAGA ACTTAACACC CAGTGGGATT ACATGTGCCG CCAGGTCTAT 
GCCAGGAAGG AAGCCTTAAA AGGAGGTTTG GATAAAACTG TAAGTCTTCA GAAAGATCTG 
TCAGAGATGC ATGAGTGGAT GACACAAGCT GAAGAAGAAT ACCTAGAGAG AGATTTCGAA 
TACAAGACCC CTGATGAATT ACAGACAGCA GTTGAAGAGA TGAAGAGAGC TAAAGAAGAG 
GCCCAGCAAA AAGAAGCAAA AGTGAAACTC CTAACCGAGT CCGTCAATAG TGTCATAGCT 
CAGGCTCCAC CTGCAGCACA AGAGGCCTTA AAAAAGGAAC TTGACACTCT CACCACCAAC 
TACCAGTGGC TCTGCACCAG GCTCAATGGC AAATGCAAGA CCTTGGAAGA AGTTTGGGCG 
TGCTGGCATG AGTTATTGTC CTACTTGGAG AAGGCAAACA AGTGGCTAAG TGAAGTAGAA 
GTCAAGCTTA AAACCACTGA AAATATTTCT GGGGGAGCTG AGGAAATCGC CGAGGTGCTT 
GATTCGCTTG AAAATTTGAT GCAACATTCA GAGGATAACC CGAATCAGAT TCGCATATTG 
GCACAGACCT TGACAGATGG TGGAGTCATG GATGAACTGA TCAATGAGGA GCTTGAGACA 
TTTAATTCTC GTTGGAGAGA ACTCCATGAA GAGGCTGTGA GGAGGCAAAA GTTGCTTGAG 
CAGAGTATCC AGTCGGCCCA GGAGATAGAA AAATCCTTGC ACTTAATTCA GGAGTCCCTC 
TCTTCCATTG ACAAGCAGTT GGCAGCTTAT ATTGCTGACA AAGTGGATGC AGCTCAGATG 
CCTCAGGAAG CCCAGAAAAT CCAATCAGAT TTGACAAGTC ATGAGATCAG TTTAGAAGAA 
ATGAAGAAAC ATAACCAGGG AAAGGAGACT GCCCAAAGGG TACTATCCCA AATTGATGTG 
GCACAGAAAA AATTGCAGGA TGTTTCCATG AAGTTTCGAT TATTCCAGAA GCCAGCCAAT 
TTTGAGCAGC GCCTACAAGA AAGTAAAATG ATTTTAGATG AAGTGAAGAT GCATTTACCT 
GCGTTGGAAA CAAAGAGTGT GGAACAGGAA GTAGTACAGT CACAGTTAAA TCATTGTGTG 
AACTTGTATA AAAGTCTGAG TGAAGTGAAG TCTGAAGTGG AAATGGTAAT AAAAACTGGA 
CGTCAGATTG TACAGAAGAA GCAGACGGAA AACCCGAAAG AGCTTGATGA AAGAGTTACA 
GCTTTGAAAT TGCATTATAA TGAGCTGGGA GCAAAGGTGA CAGAAAGAAA GCAACAGTTG 
GAAAAATGCT TGAAATTGTC CCGTAAGATG CGAAAGGAAA TGAATGCCCT GACAGAATGG 
CTGGCAGCGA CAGATATGGA ACTGACGAAG AGATCGGCAG TTGAAGGAAT GCCTAGTAAT 
TTGGATTCTG AAGTTGCCTG GGGAAAGGCT ACTCAGAAAG AGATTGAGAA ACAGAAGGTT 
CACCTAAAGA GTGTCACAGA GGTAGGAGAG GCCTTGAAAA CGGTTTTGGG CAAGAAGGAA 
ATGTTGGTGG AAGATAAACT GAGTCTTCTG AATAGTAACT GGATAGCCGT CACTTCCCGA 
GCAGAAGAGT GGTTAAACCT TTTATTGGAA TACCAGAAAC ACATGGAAAA CTTTGACCAG 
AATGTGGATT ACATCACAAA CTGGATCATT CAGGCTGATG CACTTTTGGA TGAATCTGAG 
AAAAAGAAAC CTCAGCAAAA AGAAGACATA CTTAAGCGTT TAAAGGCTGA AATGAATGAC 
ATACGTCCAA AGGTGGATTC TACACGTGAC CAAGCAGCAA ACCTGATGGC AAACCGCGGC 
GACCACTGCA GGAAAGTAGT AGAGCCCAAA ATCTCAGAGC TCAACCATCG ATTTGCAGCC 
ATTTCTCACA GAATTAAGAC TGGAAAGGCC TCCATTCCTT TGAAGGAATT GGAGCAGTTT 
AACTCAGATA TACAAAAATT GCTTGAACCA CTGGAGGCTG AAATTCAGCA GGGGGTGAAT 
CTGAAAGAGG AAGACTTCAA TAAAGATATG AGTGAAGACA ATGAGGGTAC TGTAAAAGAA 
TTGTTGCAAA GAGGAGACAA CTTACAACAA AGAATCACAG ATGAGAGAAA GCGAGAGGAA 
ATAAAGATAA AACAACAGCT GTTACAGACA AAACATAATG CTCTCAAGGA TTTGAGGTCT 
CAAAGAAGAA AAAAGGCTCT AGAAATTTCT CACCAGTGGT ATCAGTACAA GAGGCAGGCT 
GATGATCTCC TGAAATGCTT GGATGACATT GAAAAAAAAT TAGCCAGCCT ACCTGAACCC 
AGAGATGAAA GGAAAATAAA GGAAATTGAT CGTGAATTGC AGAAGAAGAA AGAGGAGCTG 
AATGCAGTGC GTAGGCAAGC TGAGGGCTTG TCTGAGGATG GGGCCGCAAT GGCAGTGGAG 
CCAACTCAGA TCCAGCTCAG CAAGCGCTGG CGGGAAATTG AGAGCAAATT TGCTCAGTTT 
CGAAGACTCA ACTTTGCACA AATTCACACT GTCCATGAAG AGTCAGTGGT GGCGATGACT 
GAAGACATGC CTTTGGAAAT TTCTTATGTG CCTTCTACTT ACCTGACTGA GATCACTCAT 
GTCTCACAAG CCCTATCAGA AGTGGAAGAG CTTCTTAATG CTCCCGACCT TTGTGCTCAA 
GATTTTGAAG ATCTCTTTAA ACAAGAGGAA TCCTTGAAGA ACATAAAAGA CAGCCTGCAA 
CAAATCTCAG GTCGGATTGA CATCATTCAC AATAAAAAGA CAGCAGCATT GCACAGTGCC 
ACTCCTGCAG AAAGGGCAAA GCTCCAGGAA GCTCTCTCAC GGCTTGATTT CCAATGGGAA 
AGAGTTAACA ATATGTACAA GGACCGACAA GGGAGATTTG ACAGATCTGT GGAAAAATGG 
CGGCGGTTTC ATTATGATAT GAAGATACTT AATCAATGGC TAACAGAAGC TGAACAGTTT 
CTCAAAAAGA CACAAATTCC TGAGAATTGG GAACATGCCA AATACAAATG GTATCTTAAG 
GAACTCCAGG ATGGCATTGG ACAGCGGCAA AGTGTTGTCA GGGTATTGAA TGCAACTGGG 
GAAGAAATAA TTCAACAGTC CTCAAAAACA GATGCCAGTA TTCTCCAAGA AAAACTGGGA 
AGCCTGAATC TGCGGTGGCA GGAGGTCTGC AAACAGCTGG CAGAAAGAAA AAAGAGGCTA 
GAGGAACAGA AGAATATCTT GTCAGAATTT CAAAGAGATG TAAATGAATT TGTTTTATGG 
TTGGAAGAAG CGGATAACGT TGCTAATATT CCACTTGAAC CTGGAAATGA GCAGCAGCTA 
AAAGAAAAAC TTGAACAAGT CAAGTTACTG GCAGAAGAGT TGCCCCTGCG CCAGGGAATT 
CTAAAACAAT TAAATGAAAC TGGAGGAACA GTGCTTGTAA GTGCTCCCCT AAGCCCAGAA 
GAGCAAGATA AACTTGAAAA TAAGCTCAAG CAGACAAATC TTCAGTGGAT AAAGGTTTCT 
AGAAATCTGC CTGAGAAGCA AGAAGAAATT GAGGCACACG TAAAAGACCT TGGACAGCTG 
GAAGAGCAGT TAAATCATCT GCTTCTATGG CTGTCTCCTA TTAGGAATCA GTTGGAAATT 
TACAATCAGC CAAATCAAAC AGGACCATTT GACATCAAGG AAATTGAAGT AGCAGTTCAA 
GCTAAACAGC CGGATGTGGA AGGGATTTTG TCTAAAGGGC AGCATTTGTA CAAGGAAAAA 
CCAGCCACTC AGCCAGCGAA GAGAAAGCTG GAAGATCTCA GCTCTGATTG GAAGGTGGTA 
ACTCAGTTGC TTCAAGAGCT GCGGGCAAAG CAGCCTGGCC CAGCTCCTGG ACTGACCACT 
GTCAGAGCCC CTCCCAGTCA GACTGTTACT CTGGTGACAC AACCCGCGGT TACCAAGGAA 
ACTGCCATCT CCAAACTAGA AATGCCATCT TCATTGCTGT TGGAGGTACC TGCACTGGCA 
GATTTCAACC GAGCTTGGAC AGAACTTACC GACTGGCTGT CTCTGCTTGA TCGAGTTATA 
AAATCACAGA GGGTGATGGT GGGTGATCTT GAAGACATTA ACGAGATGAT CATCAAGCAG 
AAGGCAACGC TGCAGGATTT GGAACAGAGG CGCCCCCAGT TGGAAGAACT CATTACCGCT 
GCCCAGAATT TGAAAAACAA GACCAGCAAT CAAGAGGCTA GAACAATCAT TACTGATCGA 
ATTGAAAGAA TTCAGAGTCA GTGGGATGAA GTACAGGAAC ATCTTCAGAA CCGGAGGCAA 
CAGTTGAATG AAATGTTAAA GGATTCCACA CAATGGCTGG AAGCTAAAGA GGAGGCTGAG 
CAGGTGTTGG GGCAGGCCAG AGCCAAGCTT GAGTCATGGA AGGAGGCTCC CTACACAGTA 
GATGCAATCC AAAAGAAAAT CACAGAAACC AAGCAGTTGG CCAAAGACCT CCGCCAGTGG 
CAGATAAATG TAGATGTTGC AAATGATTTG GCACTGAAAC TTCTCCGAGA TTATTCTGCA 
GATGATACCA GAAAAGTACA CATGATAACA GAGAACATCA ATGCCTCTTG GGCAAGCATC 
CATAAAAGAT TGAGTGAGCG AGAGGCTGCT CTGGAAGAAA CCCACAGATT ACTGCAACAG 
TTCCCCTTGG ACCTGGAGAA GTTCCTTGCC TGGCTTACAG AAGCCGAAAC AACTGCCAAC 
GTCCTGCAGG ATGCCACCCA TAAGGAAAGG CTTCTAGAAG ATTCCAAGGG AGTAAGAGAG 
CTGATGAAAC AATGGCAAGA TCTCCAAGGA GAAATCGAAG CTCACACAGA TATCTATCAC 
AACCTGGACG AAAATGGCCA AAAAGTCCTG AGATCCCTGG AAGGTTCTGA CGATGCAGCC 
TTGTTGCAAA GACGTTTGGA TAACATGAAC TTCAAGTGGA GCGAACTTCG GAAAAAGTCT 
CTCAACATTA GGTCTCACTT GGAAGCCAGT TCTGACCAGT GGAAGCGTCT GCACCTTTCT 
CTTCAGGAAC TTCTGGTATG GCTCCAGCTG AAAGATGATG AGTTAAGCCG GCAGGCACCC 
ATTGGAGGAG ACTTTCCAGC GGTGCAGAAG CAGAATGATG TACACAGGGC CTTCAAGAGG 
GAATTGAAAA CGAAAGAACC TGTAATCATG AGTACTCTTG AGACTGTACG AATATTTCTG 
ACAGAGCAGC CTTTAGAAGG ACTAGAGAAA CTCTACCAGG AGCCCAGAGA GCTGCCTCCT 
GAAGAGAGAG CCCAGAATGT CACACGGCTC CTACGAAAGC AAGCTGAGGA GGTCAACACT 
CAGTGGGAAA AACTGAACGT GCACTCTGCA GACTGGCAGA GAAAAATAGA CGAGGCCCTC 
GAAAGACTCC AGGAGCTTCA GGAAGCAACA GATGAGCTGG ATCTCAAACT ACGTCAGGCA 
GAGGTGATCA AAGGATCCTG GCAGCCTGTG GGTGATCTCC TCATTGACTC TCTCCAAGAT 
CACCTCGAAA AAGTCAAGGC GCTTCGAGGA GAAATTACAC CTCTGAAAGA GAATGTCAGC 
TACGTCAATG ACCTTGCTCG CCAACTCACT ACGTTGGGCA TTCAGCTGTC ACCATATAAC 
CTCAACACTC TGGAAGACCT GAACACCAGA TGGAAGCTTC TGCAGGTGGC CATTGAGGAC 
CGCATCAGGC AGCTGCATGA AGCGCACAGG GACTTTGGAC CAGCCTCCCA GCACTTCCTT 
TCCACTTCTG TCCAGGGTCC CTGGGAGAGA GCCATCTCAC CAAACAAAGT GCCCTACTAT 
ATCAACCACG AGACCCAAAC AACTTGCTGG GACCATCCCA AAATGACAGA GCTCTACCAG 
TCTTTAGCTG ACCTGAATAA TGTCAGGTTC TCAGCTTACA GGACTGCCAT GAAACTCCGA 
AGACTGCAGA AGGCCCTTTG CTTGGATCTC TTGAGCCTAT CGGCTGCATG CGATGCCTTG 
GACCAGCACA ACCTCAAGCA AAATGACCAG CCCATGGATA TCCTGCAGGT CATTAACTGT 
CTGACCACTA TTTATGATCG CCTAGAGCAA GAGCACAACA ATCTGGTCAA CGTCCCTCTC 
TGCGTGGATA TGTGTCTCAA TTGGCTGCTG AATGTTTATG ACACGGGACG AACGGGGAGG 
ATCCGGGTCC TGTCTTTTAA AACTGGCATC ATTTCTCTGT GTAAAGCCCA TTTGGAAGAC 
AAGTACAGAT ACCTCTTCAA GCAAGTGGCA AGTTCGACAG GATTTTGTGA CCAGCGCAGG 
CTGGGCCTCC TCCTGCATGA CTCTATCCAG ATCCCAAGAC AGTTGGGTGA AGTCGCATCG 
TTCGGGGGCA GTAACATTGA GCCGAGTGTC AGGAGCTGCT TCCAGTTTGC TAATAATAAG 
CCTGAGATCG AAGCGGCCCT CTTCCTAGAC TGGATGCGCC TGGAGCCCCA GTCCATGGTG 
TGGCTGCCTG TCCTGCACCG AGTGGCTGCC GCGGAAACTG CCAAGCACCA GGCCAAGTGC 
AACATCTGCA AGGAGTGTCC CATCATCGGA TTCAGGTACA GGAGTCTAAA GCACTTTAAT 
TATGACATCT GCCAAAGTTG CTTTTTTTCT GGTCGAGTTG CAAAAGGCCA TAAAATGCAC 
TATCCCATGG TGGAATACTG CACTCCGACT ACATCGGGAG AAGATGTCCG TGACTTTGCC 
AAGGTACTAA AAAACAAATT TCGAACCAAA AGGTATTTTG CGAAGCATCC CCGAATGGGC 
TACCTGCCAG TGCAGACTGT CTTAGAGGGG GACAACATGG AAACTCCTGT CACTCTGATC 
AACTTCTGGC CGGTAGATTC TGCGCCTGCC TCGTCCCCTC AGCTTTCACA CGATGATACT 
CATTCACGCA TTGAGCATTA TGCTAGCAGG CTAGCAGAAA TGGAAAACAG CAATGGATCT 
TATCTAAATG ATAGCATCTC TCCTAATGAG AGCATAGATG ATGAACATTT GTTAATCCAG 
CATTACTGCC AAAGTTTGAA CCAGGACTCC CCCCTGAGCC AGCCTCGTAG TCCTGCCCAG 
ATCTTGATTT CCTTAGAGAG TGAGGAAAGA GGGGAGCTAG AGAGAATCCT AGCAGATCTT 
GAGGAAGAAA ACAGAAATCT GCAAGCAGAG TATGATCGTC TAAAGCAGCA GCATGAACAC 
AAAGGCCTGT CCCCACTGCC ATCCCCTCCT GAAATGATGC CTACTTCTCC CCAAAGTCCC 
CGGGATGCTG AGCTCATCGC TGAGGCCAAG CTGCTGCGTC AACACAAAGG CCGCCTGGAA 
GCCAGGATGC AAATCCTTGA AGACCATAAC AAACAACTGG AATCCCAGTT ACACAGGCTC 
AGGCAGCTGC TGGAGCAACC CCAGGCAGAG GCCAAGGTGA ATGGTACAAC GGTGTCTTCT 
CCTTCTACCT CTCTTCAGAG GTCAGATAGC AGTCAGCCTA TGCTGCTCCG GGTGGTCGGC 
AGTCAGACTT CAGAATCCAT GGGCGAGGAA GACCTGCTCA GCCCTCCCCA GGACACAAGC 
ACAGGGTTAG AGGAAGTGAT GGAGCAGCTC AACCACTCCT TCCCTAGTTC CAGAGGACAC 
AATGTAGGAA GCCTTTTCCA CTTGGCAGAT GATTTGGGCA GAGCGATGGA GTCCTTAGTT 
TCAGTCATGA CAGATGAAGA AGGGGCAGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013966819.1
CDS	278..11350
Translation	MEDEREDVQKKTFTKWINAQFSKFGKQHIENLFSDLQDGRRLLDLLEGLTGQKLPKEKGSTRVHALNNVNKALRVLQKNNVDLVNIGSTDIVDGNHKLTLGLIWNIILHWQVKNVMKNIMAGLQQTNSEKILLSWVRQSTRNYPQVNVINFTTSWSDGLALNALIHSHRPDLFDWNSVVCQQSATQRLEHAFNIAKYQLGIEKLLDPEDVATTYPDKKSILMYITSLFQVLPQQVSIEAIQEVEMLPRPSKVTREEHFQLHHQMHYSQQITVSLAQGYERAPSSPKPRFKSYAYTQAAYVTTSDPTRSPLPSQHLETPEDKSFGRSLTETEANLDSYQTALEEVLSWLLSAEDALQAQGEISNDVEEVKEQFHTHEGYMMDLTSHQGRVGNVLQLGSQLIGTGKLSEDEETEVQEQMNLLNSRWECLRVASMEKQSNLHKVLMDLQNQQLKELNDWLTKTEERTRKMEKEPLGPDIEDLKRQVQQHKVLQEDLEQEQVRVNSLTHMVVVVDESSGDHATAALEEQLKVLGDRWANICRWTEDRWVLLQDILLKWQRFTEEQCLFSAWLSEKEDAVNKIHTTGFKDQSEVLSNLQKLAVLKTDLEKKKQTMDKLCSLNQDLLSALKNTVVAHKMEAWLDNFAQRWDNLVQKLEKSSAQISQAVTTTQPSLTQTTVMETVTMVTTREHILVKHAQEELPPPPPQKKRQIIVDSEIRKRLDVDITELHSWITRSEAVLQSPEFAIYRKEGNFSDLKEKVNAIEREKAEKFRKLQDASRSAQALVEQMVNEGVNADSIKQASEQLNSRWIEFCQLLSERLNWLEYQNNIITFYNQLQQLEQMTTTAENWLKTQPTTTSEPTAIKSQLKICKDEINRLSALQPQIERLKIQSIALKEKGQGPMFLDADFVAFTNHFNQVFADVQAREKELQTIFDSLPPMRYQETMSTILTWIQQSETKLSIPQVTVTEYDIMEQRLGELQALQSSLQEQQNGLNYLSTTVKEMSKKAPLSDISRKYQSEFEEIEGRWKKLSSQLVEHCQKLEEQMAKLRKIQNHIKTLKKWITEVDVFLKEEWPALGDSEILKRQLKQCRLLVNDIQTIQPSLNSVNEGAQKMKNEAEPEFAGRLETELRELNTQWDYMCRQVYARKEALKGGLDKTVSLQKDLSEMHEWMTQAEEEYLERDFEYKTPDELQTAVEEMKRAKEEAQQKEAKVKLLTESVNSVIAQAPPAAQEALKKELDTLTTNYQWLCTRLNGKCKTLEEVWACWHELLSYLEKANKWLSEVEVKLKTTENISGGAEEIAEVLDSLENLMQHSEDNPNQIRILAQTLTDGGVMDELINEELETFNSRWRELHEEAVRRQKLLEQSIQSAQEIEKSLHLIQESLSSIDKQLAAYIADKVDAAQMPQEAQKIQSDLTSHEISLEEMKKHNQGKETAQRVLSQIDVAQKKLQDVSMKFRLFQKPANFEQRLQESKMILDEVKMHLPALETKSVEQEVVQSQLNHCVNLYKSLSEVKSEVEMVIKTGRQIVQKKQTENPKELDERVTALKLHYNELGAKVTERKQQLEKCLKLSRKMRKEMNALTEWLAATDMELTKRSAVEGMPSNLDSEVAWGKATQKEIEKQKVHLKSVTEVGEALKTVLGKKEMLVEDKLSLLNSNWIAVTSRAEEWLNLLLEYQKHMENFDQNVDYITNWIIQADALLDESEKKKPQQKEDILKRLKAEMNDIRPKVDSTRDQAANLMANRGDHCRKVVEPKISELNHRFAAISHRIKTGKASIPLKELEQFNSDIQKLLEPLEAEIQQGVNLKEEDFNKDMSEDNEGTVKELLQRGDNLQQRITDERKREEIKIKQQLLQTKHNALKDLRSQRRKKALEISHQWYQYKRQADDLLKCLDDIEKKLASLPEPRDERKIKEIDRELQKKKEELNAVRRQAEGLSEDGAAMAVEPTQIQLSKRWREIESKFAQFRRLNFAQIHTVHEESVVAMTEDMPLEISYVPSTYLTEITHVSQALSEVEELLNAPDLCAQDFEDLFKQEESLKNIKDSLQQISGRIDIIHNKKTAALHSATPAERAKLQEALSRLDFQWERVNNMYKDRQGRFDRSVEKWRRFHYDMKILNQWLTEAEQFLKKTQIPENWEHAKYKWYLKELQDGIGQRQSVVRVLNATGEEIIQQSSKTDASILQEKLGSLNLRWQEVCKQLAERKKRLEEQKNILSEFQRDVNEFVLWLEEADNVANIPLEPGNEQQLKEKLEQVKLLAEELPLRQGILKQLNETGGTVLVSAPLSPEEQDKLENKLKQTNLQWIKVSRNLPEKQEEIEAHVKDLGQLEEQLNHLLLWLSPIRNQLEIYNQPNQTGPFDIKEIEVAVQAKQPDVEGILSKGQHLYKEKPATQPAKRKLEDLSSDWKVVTQLLQELRAKQPGPAPGLTTVRAPPSQTVTLVTQPAVTKETAISKLEMPSSLLLEVPALADFNRAWTELTDWLSLLDRVIKSQRVMVGDLEDINEMIIKQKATLQDLEQRRPQLEELITAAQNLKNKTSNQEARTIITDRIERIQSQWDEVQEHLQNRRQQLNEMLKDSTQWLEAKEEAEQVLGQARAKLESWKEAPYTVDAIQKKITETKQLAKDLRQWQINVDVANDLALKLLRDYSADDTRKVHMITENINASWASIHKRLSEREAALEETHRLLQQFPLDLEKFLAWLTEAETTANVLQDATHKERLLEDSKGVRELMKQWQDLQGEIEAHTDIYHNLDENGQKVLRSLEGSDDAALLQRRLDNMNFKWSELRKKSLNIRSHLEASSDQWKRLHLSLQELLVWLQLKDDELSRQAPIGGDFPAVQKQNDVHRAFKRELKTKEPVIMSTLETVRIFLTEQPLEGLEKLYQEPRELPPEERAQNVTRLLRKQAEEVNTQWEKLNVHSADWQRKIDEALERLQELQEATDELDLKLRQAEVIKGSWQPVGDLLIDSLQDHLEKVKALRGEITPLKENVSYVNDLARQLTTLGIQLSPYNLNTLEDLNTRWKLLQVAIEDRIRQLHEAHRDFGPASQHFLSTSVQGPWERAISPNKVPYYINHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQVINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPVTLINFWPVDSAPASSPQLSHDDTHSRIEHYASRLAEMENSNGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKQQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDTSTGLEEVMEQLNHSFPSSRGHNVGSLFHLADDLGRAMESLVSVMTDEEGAE

Target ORF information:

RefSeq Version	XM_014111344.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris dystrophin (DMD), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111344.1

ORF Insert Sequence:

ATGGAAGATG AAAGAGAAGA TGTTCAAAAG AAAACATTCA CAAAATGGAT AAATGCACAG 
TTTTCTAAGT TTGGGAAGCA GCACATAGAG AACCTCTTCA GTGACCTACA GGATGGGAGA 
CGCCTCCTAG ACCTTTTGGA AGGCCTGACA GGGCAAAAAC TGCCAAAAGA AAAAGGATCC 
ACAAGAGTTC ATGCCCTGAA CAATGTCAAC AAGGCACTGC GCGTCTTGCA GAAAAATAAT 
GTTGATTTAG TGAACATTGG AAGTACTGAC ATAGTAGATG GAAATCACAA ACTGACTCTT 
GGTTTGATTT GGAATATAAT CCTCCACTGG CAGGTCAAAA ATGTAATGAA AAATATCATG 
GCTGGATTGC AACAAACCAA CAGTGAAAAG ATTCTCCTGA GCTGGGTCCG ACAATCAACT 
CGTAATTATC CACAGGTTAA TGTCATTAAC TTCACCACCA GCTGGTCTGA TGGCCTGGCT 
TTGAACGCTC TCATCCACAG TCATAGGCCA GACCTGTTTG ATTGGAATAG TGTGGTTTGC 
CAGCAGTCAG CCACACAACG CCTGGAACAT GCATTCAACA TTGCCAAATA TCAATTAGGC 
ATAGAGAAAC TGCTTGATCC TGAAGATGTT GCCACCACTT ATCCAGATAA GAAGTCCATC 
TTAATGTATA TCACATCACT CTTCCAAGTT TTGCCTCAAC AAGTGAGCAT TGAAGCCATC 
CAGGAAGTGG AAATGTTGCC AAGGCCATCA AAAGTTACTA GAGAAGAACA TTTTCAATTA 
CATCATCAAA TGCACTATTC TCAACAGATC ACAGTCAGTC TAGCACAGGG CTATGAACGA 
GCCCCTTCCT CTCCTAAGCC TCGGTTCAAG AGCTATGCCT ACACACAGGC TGCTTATGTC 
ACCACTTCTG ACCCCACACG GAGCCCACTT CCTTCACAGC ATTTGGAAAC TCCTGAAGAC 
AAGTCATTTG GCCGGTCATT GACAGAGACC GAAGCAAACC TGGACAGTTA TCAAACAGCT 
TTGGAAGAAG TACTCTCGTG GCTTCTTTCA GCTGAGGATG CACTGCAAGC CCAAGGAGAG 
ATTTCTAATG ATGTCGAAGA AGTGAAAGAA CAATTTCATA CTCATGAGGG ATATATGATG 
GACTTGACAT CCCATCAGGG ACGGGTCGGT AATGTTCTCC AACTGGGAAG TCAACTGATT 
GGAACAGGGA AATTATCAGA AGATGAAGAA ACCGAAGTGC AGGAACAAAT GAATCTCCTC 
AATTCAAGAT GGGAATGCCT CAGGGTAGCT AGCATGGAAA AACAAAGCAA TTTACATAAA 
GTTCTAATGG ATCTCCAGAA TCAGCAACTG AAAGAGTTAA ATGACTGGCT AACCAAAACA 
GAAGAGAGAA CAAGGAAAAT GGAGAAGGAG CCCCTTGGAC CTGATATTGA AGACCTAAAA 
CGCCAAGTAC AACAACATAA GGTGCTTCAA GAAGACTTAG AACAGGAACA AGTCAGGGTC 
AATTCCCTCA CTCATATGGT GGTGGTAGTC GATGAATCTA GTGGAGACCA TGCAACTGCT 
GCTTTGGAAG AACAACTTAA GGTACTGGGA GATCGATGGG CAAACATCTG TAGGTGGACA 
GAAGATCGCT GGGTTCTTTT ACAAGACATC CTCCTAAAAT GGCAGCGTTT TACTGAAGAA 
CAGTGCCTTT TCAGTGCATG GCTTTCGGAG AAGGAAGATG CAGTGAACAA GATTCACACA 
ACTGGCTTTA AGGATCAAAG TGAAGTGTTA TCAAATCTTC AGAAACTGGC TGTCTTAAAA 
ACAGATCTGG AAAAGAAGAA GCAAACCATG GACAAACTCT GCTCACTCAA CCAAGACCTT 
CTTTCAGCGC TGAAAAACAC AGTGGTAGCC CACAAGATGG AAGCATGGCT GGACAACTTT 
GCCCAGCGCT GGGATAATTT AGTCCAGAAA CTTGAAAAAA GTTCAGCACA GATTTCACAG 
GCTGTCACCA CCACTCAGCC ATCACTAACA CAGACAACTG TAATGGAAAC AGTAACTATG 
GTGACCACGA GGGAACACAT CTTGGTAAAG CATGCCCAAG AGGAACTGCC ACCACCACCC 
CCTCAGAAGA AGAGGCAGAT TATCGTGGAT TCTGAAATTA GGAAAAGGTT GGATGTCGAT 
ATAACTGAAC TTCACAGTTG GATTACTCGT TCAGAAGCTG TGTTGCAGAG TCCTGAATTT 
GCAATCTATC GGAAGGAAGG CAACTTCTCA GACCTTAAAG AAAAAGTCAA TGCCATAGAG 
CGAGAAAAAG CCGAGAAGTT CAGAAAACTG CAAGATGCCA GCAGATCAGC TCAGGCCCTG 
GTGGAACAGA TGGTGAATGA GGGTGTTAAT GCTGACAGCA TCAAACAAGC CTCCGAACAA 
CTGAACAGCC GGTGGATAGA GTTCTGCCAA TTGCTAAGCG AGAGACTTAA CTGGCTGGAG 
TATCAGAACA ACATCATCAC TTTCTATAAT CAGCTACAAC AATTGGAGCA GATGACAACT 
ACTGCTGAAA ACTGGTTGAA AACCCAGCCT ACCACCACAT CAGAGCCAAC AGCAATTAAA 
AGCCAGTTAA AAATTTGTAA GGATGAAATC AACCGACTGT CAGCTCTTCA GCCTCAAATC 
GAGCGATTAA AAATTCAAAG CATAGCCCTG AAAGAGAAAG GACAAGGGCC AATGTTCCTG 
GATGCAGACT TTGTGGCCTT TACAAATCAT TTTAACCAAG TCTTTGCTGA TGTGCAGGCA 
AGAGAAAAAG AGCTACAAAC AATTTTTGAC AGTTTGCCAC CCATGCGCTA TCAGGAGACT 
ATGAGTACCA TCCTGACATG GATCCAGCAG TCAGAAACCA AACTCTCTAT ACCTCAGGTT 
ACTGTCACTG AATATGACAT CATGGAACAG AGACTCGGAG AGCTACAGGC TTTACAAAGC 
TCTCTGCAAG AGCAACAAAA TGGCCTAAAC TATCTCAGCA CCACTGTGAA AGAGATGTCA 
AAGAAAGCAC CACTGTCTGA TATTAGTCGG AAATATCAAT CAGAATTTGA AGAGATTGAG 
GGACGTTGGA AGAAGCTGTC TTCCCAGCTG GTTGAACATT GTCAAAAGTT GGAGGAGCAA 
ATGGCTAAAC TTCGAAAAAT TCAGAATCAC ATAAAAACTC TGAAGAAATG GATCACTGAA 
GTCGATGTTT TCCTGAAGGA GGAATGGCCT GCCCTTGGGG ATTCAGAAAT TCTGAAAAGA 
CAGCTGAAAC AGTGCAGGCT TTTAGTCAAT GACATTCAGA CCATCCAGCC TAGTCTCAAC 
AGTGTCAATG AAGGGGCTCA GAAGATGAAG AATGAAGCAG AACCAGAGTT TGCTGGCAGA 
CTTGAGACAG AGCTCCGAGA ACTTAACACC CAGTGGGATT ACATGTGCCG CCAGGTCTAT 
GCCAGGAAGG AAGCCTTAAA AGGAGGTTTG GATAAAACTG TAAGTCTTCA GAAAGATCTG 
TCAGAGATGC ATGAGTGGAT GACACAAGCT GAAGAAGAAT ACCTAGAGAG AGATTTCGAA 
TACAAGACCC CTGATGAATT ACAGACAGCA GTTGAAGAGA TGAAGAGAGC TAAAGAAGAG 
GCCCAGCAAA AAGAAGCAAA AGTGAAACTC CTAACCGAGT CCGTCAATAG TGTCATAGCT 
CAGGCTCCAC CTGCAGCACA AGAGGCCTTA AAAAAGGAAC TTGACACTCT CACCACCAAC 
TACCAGTGGC TCTGCACCAG GCTCAATGGC AAATGCAAGA CCTTGGAAGA AGTTTGGGCG 
TGCTGGCATG AGTTATTGTC CTACTTGGAG AAGGCAAACA AGTGGCTAAG TGAAGTAGAA 
GTCAAGCTTA AAACCACTGA AAATATTTCT GGGGGAGCTG AGGAAATCGC CGAGGTGCTT 
GATTCGCTTG AAAATTTGAT GCAACATTCA GAGGATAACC CGAATCAGAT TCGCATATTG 
GCACAGACCT TGACAGATGG TGGAGTCATG GATGAACTGA TCAATGAGGA GCTTGAGACA 
TTTAATTCTC GTTGGAGAGA ACTCCATGAA GAGGCTGTGA GGAGGCAAAA GTTGCTTGAG 
CAGAGTATCC AGTCGGCCCA GGAGATAGAA AAATCCTTGC ACTTAATTCA GGAGTCCCTC 
TCTTCCATTG ACAAGCAGTT GGCAGCTTAT ATTGCTGACA AAGTGGATGC AGCTCAGATG 
CCTCAGGAAG CCCAGAAAAT CCAATCAGAT TTGACAAGTC ATGAGATCAG TTTAGAAGAA 
ATGAAGAAAC ATAACCAGGG AAAGGAGACT GCCCAAAGGG TACTATCCCA AATTGATGTG 
GCACAGAAAA AATTGCAGGA TGTTTCCATG AAGTTTCGAT TATTCCAGAA GCCAGCCAAT 
TTTGAGCAGC GCCTACAAGA AAGTAAAATG ATTTTAGATG AAGTGAAGAT GCATTTACCT 
GCGTTGGAAA CAAAGAGTGT GGAACAGGAA GTAGTACAGT CACAGTTAAA TCATTGTGTG 
AACTTGTATA AAAGTCTGAG TGAAGTGAAG TCTGAAGTGG AAATGGTAAT AAAAACTGGA 
CGTCAGATTG TACAGAAGAA GCAGACGGAA AACCCGAAAG AGCTTGATGA AAGAGTTACA 
GCTTTGAAAT TGCATTATAA TGAGCTGGGA GCAAAGGTGA CAGAAAGAAA GCAACAGTTG 
GAAAAATGCT TGAAATTGTC CCGTAAGATG CGAAAGGAAA TGAATGCCCT GACAGAATGG 
CTGGCAGCGA CAGATATGGA ACTGACGAAG AGATCGGCAG TTGAAGGAAT GCCTAGTAAT 
TTGGATTCTG AAGTTGCCTG GGGAAAGGCT ACTCAGAAAG AGATTGAGAA ACAGAAGGTT 
CACCTAAAGA GTGTCACAGA GGTAGGAGAG GCCTTGAAAA CGGTTTTGGG CAAGAAGGAA 
ATGTTGGTGG AAGATAAACT GAGTCTTCTG AATAGTAACT GGATAGCCGT CACTTCCCGA 
GCAGAAGAGT GGTTAAACCT TTTATTGGAA TACCAGAAAC ACATGGAAAA CTTTGACCAG 
AATGTGGATT ACATCACAAA CTGGATCATT CAGGCTGATG CACTTTTGGA TGAATCTGAG 
AAAAAGAAAC CTCAGCAAAA AGAAGACATA CTTAAGCGTT TAAAGGCTGA AATGAATGAC 
ATACGTCCAA AGGTGGATTC TACACGTGAC CAAGCAGCAA ACCTGATGGC AAACCGCGGC 
GACCACTGCA GGAAAGTAGT AGAGCCCAAA ATCTCAGAGC TCAACCATCG ATTTGCAGCC 
ATTTCTCACA GAATTAAGAC TGGAAAGGCC TCCATTCCTT TGAAGGAATT GGAGCAGTTT 
AACTCAGATA TACAAAAATT GCTTGAACCA CTGGAGGCTG AAATTCAGCA GGGGGTGAAT 
CTGAAAGAGG AAGACTTCAA TAAAGATATG AGTGAAGACA ATGAGGGTAC TGTAAAAGAA 
TTGTTGCAAA GAGGAGACAA CTTACAACAA AGAATCACAG ATGAGAGAAA GCGAGAGGAA 
ATAAAGATAA AACAACAGCT GTTACAGACA AAACATAATG CTCTCAAGGA TTTGAGGTCT 
CAAAGAAGAA AAAAGGCTCT AGAAATTTCT CACCAGTGGT ATCAGTACAA GAGGCAGGCT 
GATGATCTCC TGAAATGCTT GGATGACATT GAAAAAAAAT TAGCCAGCCT ACCTGAACCC 
AGAGATGAAA GGAAAATAAA GGAAATTGAT CGTGAATTGC AGAAGAAGAA AGAGGAGCTG 
AATGCAGTGC GTAGGCAAGC TGAGGGCTTG TCTGAGGATG GGGCCGCAAT GGCAGTGGAG 
CCAACTCAGA TCCAGCTCAG CAAGCGCTGG CGGGAAATTG AGAGCAAATT TGCTCAGTTT 
CGAAGACTCA ACTTTGCACA AATTCACACT GTCCATGAAG AGTCAGTGGT GGCGATGACT 
GAAGACATGC CTTTGGAAAT TTCTTATGTG CCTTCTACTT ACCTGACTGA GATCACTCAT 
GTCTCACAAG CCCTATCAGA AGTGGAAGAG CTTCTTAATG CTCCCGACCT TTGTGCTCAA 
GATTTTGAAG ATCTCTTTAA ACAAGAGGAA TCCTTGAAGA ACATAAAAGA CAGCCTGCAA 
CAAATCTCAG GTCGGATTGA CATCATTCAC AATAAAAAGA CAGCAGCATT GCACAGTGCC 
ACTCCTGCAG AAAGGGCAAA GCTCCAGGAA GCTCTCTCAC GGCTTGATTT CCAATGGGAA 
AGAGTTAACA ATATGTACAA GGACCGACAA GGGAGATTTG ACAGATCTGT GGAAAAATGG 
CGGCGGTTTC ATTATGATAT GAAGATACTT AATCAATGGC TAACAGAAGC TGAACAGTTT 
CTCAAAAAGA CACAAATTCC TGAGAATTGG GAACATGCCA AATACAAATG GTATCTTAAG 
GAACTCCAGG ATGGCATTGG ACAGCGGCAA AGTGTTGTCA GGGTATTGAA TGCAACTGGG 
GAAGAAATAA TTCAACAGTC CTCAAAAACA GATGCCAGTA TTCTCCAAGA AAAACTGGGA 
AGCCTGAATC TGCGGTGGCA GGAGGTCTGC AAACAGCTGG CAGAAAGAAA AAAGAGGCTA 
GAGGAACAGA AGAATATCTT GTCAGAATTT CAAAGAGATG TAAATGAATT TGTTTTATGG 
TTGGAAGAAG CGGATAACGT TGCTAATATT CCACTTGAAC CTGGAAATGA GCAGCAGCTA 
AAAGAAAAAC TTGAACAAGT CAAGTTACTG GCAGAAGAGT TGCCCCTGCG CCAGGGAATT 
CTAAAACAAT TAAATGAAAC TGGAGGAACA GTGCTTGTAA GTGCTCCCCT AAGCCCAGAA 
GAGCAAGATA AACTTGAAAA TAAGCTCAAG CAGACAAATC TTCAGTGGAT AAAGGTTTCT 
AGAAATCTGC CTGAGAAGCA AGAAGAAATT GAGGCACACG TAAAAGACCT TGGACAGCTG 
GAAGAGCAGT TAAATCATCT GCTTCTATGG CTGTCTCCTA TTAGGAATCA GTTGGAAATT 
TACAATCAGC CAAATCAAAC AGGACCATTT GACATCAAGG AAATTGAAGT AGCAGTTCAA 
GCTAAACAGC CGGATGTGGA AGGGATTTTG TCTAAAGGGC AGCATTTGTA CAAGGAAAAA 
CCAGCCACTC AGCCAGCGAA GAGAAAGCTG GAAGATCTCA GCTCTGATTG GAAGGTGGTA 
ACTCAGTTGC TTCAAGAGCT GCGGGCAAAG CAGCCTGGCC CAGCTCCTGG ACTGACCACT 
GTCAGAGCCC CTCCCAGTCA GACTGTTACT CTGGTGACAC AACCCGCGGT TACCAAGGAA 
ACTGCCATCT CCAAACTAGA AATGCCATCT TCATTGCTGT TGGAGGTACC TGCACTGGCA 
GATTTCAACC GAGCTTGGAC AGAACTTACC GACTGGCTGT CTCTGCTTGA TCGAGTTATA 
AAATCACAGA GGGTGATGGT GGGTGATCTT GAAGACATTA ACGAGATGAT CATCAAGCAG 
AAGGCAACGC TGCAGGATTT GGAACAGAGG CGCCCCCAGT TGGAAGAACT CATTACCGCT 
GCCCAGAATT TGAAAAACAA GACCAGCAAT CAAGAGGCTA GAACAATCAT TACTGATCGA 
ATTGAAAGAA TTCAGAGTCA GTGGGATGAA GTACAGGAAC ATCTTCAGAA CCGGAGGCAA 
CAGTTGAATG AAATGTTAAA GGATTCCACA CAATGGCTGG AAGCTAAAGA GGAGGCTGAG 
CAGGTGTTGG GGCAGGCCAG AGCCAAGCTT GAGTCATGGA AGGAGGCTCC CTACACAGTA 
GATGCAATCC AAAAGAAAAT CACAGAAACC AAGCAGTTGG CCAAAGACCT CCGCCAGTGG 
CAGATAAATG TAGATGTTGC AAATGATTTG GCACTGAAAC TTCTCCGAGA TTATTCTGCA 
GATGATACCA GAAAAGTACA CATGATAACA GAGAACATCA ATGCCTCTTG GGCAAGCATC 
CATAAAAGAT TGAGTGAGCG AGAGGCTGCT CTGGAAGAAA CCCACAGATT ACTGCAACAG 
TTCCCCTTGG ACCTGGAGAA GTTCCTTGCC TGGCTTACAG AAGCCGAAAC AACTGCCAAC 
GTCCTGCAGG ATGCCACCCA TAAGGAAAGG CTTCTAGAAG ATTCCAAGGG AGTAAGAGAG 
CTGATGAAAC AATGGCAAGA TCTCCAAGGA GAAATCGAAG CTCACACAGA TATCTATCAC 
AACCTGGACG AAAATGGCCA AAAAGTCCTG AGATCCCTGG AAGGTTCTGA CGATGCAGCC 
TTGTTGCAAA GACGTTTGGA TAACATGAAC TTCAAGTGGA GCGAACTTCG GAAAAAGTCT 
CTCAACATTA GGTCTCACTT GGAAGCCAGT TCTGACCAGT GGAAGCGTCT GCACCTTTCT 
CTTCAGGAAC TTCTGGTATG GCTCCAGCTG AAAGATGATG AGTTAAGCCG GCAGGCACCC 
ATTGGAGGAG ACTTTCCAGC GGTGCAGAAG CAGAATGATG TACACAGGGC CTTCAAGAGG 
GAATTGAAAA CGAAAGAACC TGTAATCATG AGTACTCTTG AGACTGTACG AATATTTCTG 
ACAGAGCAGC CTTTAGAAGG ACTAGAGAAA CTCTACCAGG AGCCCAGAGA GCTGCCTCCT 
GAAGAGAGAG CCCAGAATGT CACACGGCTC CTACGAAAGC AAGCTGAGGA GGTCAACACT 
CAGTGGGAAA AACTGAACGT GCACTCTGCA GACTGGCAGA GAAAAATAGA CGAGGCCCTC 
GAAAGACTCC AGGAGCTTCA GGAAGCAACA GATGAGCTGG ATCTCAAACT ACGTCAGGCA 
GAGGTGATCA AAGGATCCTG GCAGCCTGTG GGTGATCTCC TCATTGACTC TCTCCAAGAT 
CACCTCGAAA AAGTCAAGGC GCTTCGAGGA GAAATTACAC CTCTGAAAGA GAATGTCAGC 
TACGTCAATG ACCTTGCTCG CCAACTCACT ACGTTGGGCA TTCAGCTGTC ACCATATAAC 
CTCAACACTC TGGAAGACCT GAACACCAGA TGGAAGCTTC TGCAGGTGGC CATTGAGGAC 
CGCATCAGGC AGCTGCATGA AGCGCACAGG GACTTTGGAC CAGCCTCCCA GCACTTCCTT 
TCCACTTCTG TCCAGGGTCC CTGGGAGAGA GCCATCTCAC CAAACAAAGT GCCCTACTAT 
ATCAACCACG AGACCCAAAC AACTTGCTGG GACCATCCCA AAATGACAGA GCTCTACCAG 
TCTTTAGCTG ACCTGAATAA TGTCAGGTTC TCAGCTTACA GGACTGCCAT GAAACTCCGA 
AGACTGCAGA AGGCCCTTTG CTTGGATCTC TTGAGCCTAT CGGCTGCATG CGATGCCTTG 
GACCAGCACA ACCTCAAGCA AAATGACCAG CCCATGGATA TCCTGCAGGT CATTAACTGT 
CTGACCACTA TTTATGATCG CCTAGAGCAA GAGCACAACA ATCTGGTCAA CGTCCCTCTC 
TGCGTGGATA TGTGTCTCAA TTGGCTGCTG AATGTTTATG ACACGGGACG AACGGGGAGG 
ATCCGGGTCC TGTCTTTTAA AACTGGCATC ATTTCTCTGT GTAAAGCCCA TTTGGAAGAC 
AAGTACAGAT ACCTCTTCAA GCAAGTGGCA AGTTCGACAG GATTTTGTGA CCAGCGCAGG 
CTGGGCCTCC TCCTGCATGA CTCTATCCAG ATCCCAAGAC AGTTGGGTGA AGTCGCATCG 
TTCGGGGGCA GTAACATTGA GCCGAGTGTC AGGAGCTGCT TCCAGTTTGC TAATAATAAG 
CCTGAGATCG AAGCGGCCCT CTTCCTAGAC TGGATGCGCC TGGAGCCCCA GTCCATGGTG 
TGGCTGCCTG TCCTGCACCG AGTGGCTGCC GCGGAAACTG CCAAGCACCA GGCCAAGTGC 
AACATCTGCA AGGAGTGTCC CATCATCGGA TTCAGGTACA GGAGTCTAAA GCACTTTAAT 
TATGACATCT GCCAAAGTTG CTTTTTTTCT GGTCGAGTTG CAAAAGGCCA TAAAATGCAC 
TATCCCATGG TGGAATACTG CACTCCGACT ACATCGGGAG AAGATGTCCG TGACTTTGCC 
AAGGTACTAA AAAACAAATT TCGAACCAAA AGGTATTTTG CGAAGCATCC CCGAATGGGC 
TACCTGCCAG TGCAGACTGT CTTAGAGGGG GACAACATGG AAACTCCTGT CACTCTGATC 
AACTTCTGGC CGGTAGATTC TGCGCCTGCC TCGTCCCCTC AGCTTTCACA CGATGATACT 
CATTCACGCA TTGAGCATTA TGCTAGCAGG CTAGCAGAAA TGGAAAACAG CAATGGATCT 
TATCTAAATG ATAGCATCTC TCCTAATGAG AGCATAGATG ATGAACATTT GTTAATCCAG 
CATTACTGCC AAAGTTTGAA CCAGGACTCC CCCCTGAGCC AGCCTCGTAG TCCTGCCCAG 
ATCTTGATTT CCTTAGAGAG TGAGGAAAGA GGGGAGCTAG AGAGAATCCT AGCAGATCTT 
GAGGAAGAAA ACAGAAATCT GCAAGCAGAG TATGATCGTC TAAAGCAGCA GCATGAACAC 
AAAGGCCTGT CCCCACTGCC ATCCCCTCCT GAAATGATGC CTACTTCTCC CCAAAGTCCC 
CGGGATGCTG AGCTCATCGC TGAGGCCAAG CTGCTGCGTC AACACAAAGG CCGCCTGGAA 
GCCAGGATGC AAATCCTTGA AGACCATAAC AAACAACTGG AATCCCAGTT ACACAGGCTC 
AGGCAGCTGC TGGAGCAACC CCAGGCAGAG GCCAAGGTGA ATGGTACAAC GGTGTCTTCT 
CCTTCTACCT CTCTTCAGAG GTCAGATAGC AGTCAGCCTA TGCTGCTCCG GGTGGTCGGC 
AGTCAGACTT CAGAATCCAT GGGCGAGGAA GACCTGCTCA GCCCTCCCCA GGACACAAGC 
ACAGGGTTAG AGGAAGTGAT GGAGCAGCTC AACCACTCCT TCCCTAGTTC CAGAGGACAC 
AATGTAGGAA GCCTTTTCCA CTTGGCAGAT GATTTGGGCA GAGCGATGGA GTCCTTAGTT 
TCAGTCATGA CAGATGAAGA AGGGGCAGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMD ( XM_014111344.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013966819.1 Canis lupus familiaris dystrophin (DMD), transcript variant X4, mRNA.

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CloneID	OCe45415
Clone ID Related Accession (Same CDS sequence)	XM_014111344.1 , XM_014111344.2
Accession Version	XM_014111344.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	dystrophin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014111344.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGATG AAAGAGAAGA TGTTCAAAAG AAAACATTCA CAAAATGGAT AAATGCACAG 
TTTTCTAAGT TTGGGAAGCA GCACATAGAG AACCTCTTCA GTGACCTACA GGATGGGAGA 
CGCCTCCTAG ACCTTTTGGA AGGCCTGACA GGGCAAAAAC TGCCAAAAGA AAAAGGATCC 
ACAAGAGTTC ATGCCCTGAA CAATGTCAAC AAGGCACTGC GCGTCTTGCA GAAAAATAAT 
GTTGATTTAG TGAACATTGG AAGTACTGAC ATAGTAGATG GAAATCACAA ACTGACTCTT 
GGTTTGATTT GGAATATAAT CCTCCACTGG CAGGTCAAAA ATGTAATGAA AAATATCATG 
GCTGGATTGC AACAAACCAA CAGTGAAAAG ATTCTCCTGA GCTGGGTCCG ACAATCAACT 
CGTAATTATC CACAGGTTAA TGTCATTAAC TTCACCACCA GCTGGTCTGA TGGCCTGGCT 
TTGAACGCTC TCATCCACAG TCATAGGCCA GACCTGTTTG ATTGGAATAG TGTGGTTTGC 
CAGCAGTCAG CCACACAACG CCTGGAACAT GCATTCAACA TTGCCAAATA TCAATTAGGC 
ATAGAGAAAC TGCTTGATCC TGAAGATGTT GCCACCACTT ATCCAGATAA GAAGTCCATC 
TTAATGTATA TCACATCACT CTTCCAAGTT TTGCCTCAAC AAGTGAGCAT TGAAGCCATC 
CAGGAAGTGG AAATGTTGCC AAGGCCATCA AAAGTTACTA GAGAAGAACA TTTTCAATTA 
CATCATCAAA TGCACTATTC TCAACAGATC ACAGTCAGTC TAGCACAGGG CTATGAACGA 
GCCCCTTCCT CTCCTAAGCC TCGGTTCAAG AGCTATGCCT ACACACAGGC TGCTTATGTC 
ACCACTTCTG ACCCCACACG GAGCCCACTT CCTTCACAGC ATTTGGAAAC TCCTGAAGAC 
AAGTCATTTG GCCGGTCATT GACAGAGACC GAAGCAAACC TGGACAGTTA TCAAACAGCT 
TTGGAAGAAG TACTCTCGTG GCTTCTTTCA GCTGAGGATG CACTGCAAGC CCAAGGAGAG 
ATTTCTAATG ATGTCGAAGA AGTGAAAGAA CAATTTCATA CTCATGAGGG ATATATGATG 
GACTTGACAT CCCATCAGGG ACGGGTCGGT AATGTTCTCC AACTGGGAAG TCAACTGATT 
GGAACAGGGA AATTATCAGA AGATGAAGAA ACCGAAGTGC AGGAACAAAT GAATCTCCTC 
AATTCAAGAT GGGAATGCCT CAGGGTAGCT AGCATGGAAA AACAAAGCAA TTTACATAAA 
GTTCTAATGG ATCTCCAGAA TCAGCAACTG AAAGAGTTAA ATGACTGGCT AACCAAAACA 
GAAGAGAGAA CAAGGAAAAT GGAGAAGGAG CCCCTTGGAC CTGATATTGA AGACCTAAAA 
CGCCAAGTAC AACAACATAA GGTGCTTCAA GAAGACTTAG AACAGGAACA AGTCAGGGTC 
AATTCCCTCA CTCATATGGT GGTGGTAGTC GATGAATCTA GTGGAGACCA TGCAACTGCT 
GCTTTGGAAG AACAACTTAA GGTACTGGGA GATCGATGGG CAAACATCTG TAGGTGGACA 
GAAGATCGCT GGGTTCTTTT ACAAGACATC CTCCTAAAAT GGCAGCGTTT TACTGAAGAA 
CAGTGCCTTT TCAGTGCATG GCTTTCGGAG AAGGAAGATG CAGTGAACAA GATTCACACA 
ACTGGCTTTA AGGATCAAAG TGAAGTGTTA TCAAATCTTC AGAAACTGGC TGTCTTAAAA 
ACAGATCTGG AAAAGAAGAA GCAAACCATG GACAAACTCT GCTCACTCAA CCAAGACCTT 
CTTTCAGCGC TGAAAAACAC AGTGGTAGCC CACAAGATGG AAGCATGGCT GGACAACTTT 
GCCCAGCGCT GGGATAATTT AGTCCAGAAA CTTGAAAAAA GTTCAGCACA GATTTCACAG 
GCTGTCACCA CCACTCAGCC ATCACTAACA CAGACAACTG TAATGGAAAC AGTAACTATG 
GTGACCACGA GGGAACACAT CTTGGTAAAG CATGCCCAAG AGGAACTGCC ACCACCACCC 
CCTCAGAAGA AGAGGCAGAT TATCGTGGAT TCTGAAATTA GGAAAAGGTT GGATGTCGAT 
ATAACTGAAC TTCACAGTTG GATTACTCGT TCAGAAGCTG TGTTGCAGAG TCCTGAATTT 
GCAATCTATC GGAAGGAAGG CAACTTCTCA GACCTTAAAG AAAAAGTCAA TGCCATAGAG 
CGAGAAAAAG CCGAGAAGTT CAGAAAACTG CAAGATGCCA GCAGATCAGC TCAGGCCCTG 
GTGGAACAGA TGGTGAATGA GGGTGTTAAT GCTGACAGCA TCAAACAAGC CTCCGAACAA 
CTGAACAGCC GGTGGATAGA GTTCTGCCAA TTGCTAAGCG AGAGACTTAA CTGGCTGGAG 
TATCAGAACA ACATCATCAC TTTCTATAAT CAGCTACAAC AATTGGAGCA GATGACAACT 
ACTGCTGAAA ACTGGTTGAA AACCCAGCCT ACCACCACAT CAGAGCCAAC AGCAATTAAA 
AGCCAGTTAA AAATTTGTAA GGATGAAATC AACCGACTGT CAGCTCTTCA GCCTCAAATC 
GAGCGATTAA AAATTCAAAG CATAGCCCTG AAAGAGAAAG GACAAGGGCC AATGTTCCTG 
GATGCAGACT TTGTGGCCTT TACAAATCAT TTTAACCAAG TCTTTGCTGA TGTGCAGGCA 
AGAGAAAAAG AGCTACAAAC AATTTTTGAC AGTTTGCCAC CCATGCGCTA TCAGGAGACT 
ATGAGTACCA TCCTGACATG GATCCAGCAG TCAGAAACCA AACTCTCTAT ACCTCAGGTT 
ACTGTCACTG AATATGACAT CATGGAACAG AGACTCGGAG AGCTACAGGC TTTACAAAGC 
TCTCTGCAAG AGCAACAAAA TGGCCTAAAC TATCTCAGCA CCACTGTGAA AGAGATGTCA 
AAGAAAGCAC CACTGTCTGA TATTAGTCGG AAATATCAAT CAGAATTTGA AGAGATTGAG 
GGACGTTGGA AGAAGCTGTC TTCCCAGCTG GTTGAACATT GTCAAAAGTT GGAGGAGCAA 
ATGGCTAAAC TTCGAAAAAT TCAGAATCAC ATAAAAACTC TGAAGAAATG GATCACTGAA 
GTCGATGTTT TCCTGAAGGA GGAATGGCCT GCCCTTGGGG ATTCAGAAAT TCTGAAAAGA 
CAGCTGAAAC AGTGCAGGCT TTTAGTCAAT GACATTCAGA CCATCCAGCC TAGTCTCAAC 
AGTGTCAATG AAGGGGCTCA GAAGATGAAG AATGAAGCAG AACCAGAGTT TGCTGGCAGA 
CTTGAGACAG AGCTCCGAGA ACTTAACACC CAGTGGGATT ACATGTGCCG CCAGGTCTAT 
GCCAGGAAGG AAGCCTTAAA AGGAGGTTTG GATAAAACTG TAAGTCTTCA GAAAGATCTG 
TCAGAGATGC ATGAGTGGAT GACACAAGCT GAAGAAGAAT ACCTAGAGAG AGATTTCGAA 
TACAAGACCC CTGATGAATT ACAGACAGCA GTTGAAGAGA TGAAGAGAGC TAAAGAAGAG 
GCCCAGCAAA AAGAAGCAAA AGTGAAACTC CTAACCGAGT CCGTCAATAG TGTCATAGCT 
CAGGCTCCAC CTGCAGCACA AGAGGCCTTA AAAAAGGAAC TTGACACTCT CACCACCAAC 
TACCAGTGGC TCTGCACCAG GCTCAATGGC AAATGCAAGA CCTTGGAAGA AGTTTGGGCG 
TGCTGGCATG AGTTATTGTC CTACTTGGAG AAGGCAAACA AGTGGCTAAG TGAAGTAGAA 
GTCAAGCTTA AAACCACTGA AAATATTTCT GGGGGAGCTG AGGAAATCGC CGAGGTGCTT 
GATTCGCTTG AAAATTTGAT GCAACATTCA GAGGATAACC CGAATCAGAT TCGCATATTG 
GCACAGACCT TGACAGATGG TGGAGTCATG GATGAACTGA TCAATGAGGA GCTTGAGACA 
TTTAATTCTC GTTGGAGAGA ACTCCATGAA GAGGCTGTGA GGAGGCAAAA GTTGCTTGAG 
CAGAGTATCC AGTCGGCCCA GGAGATAGAA AAATCCTTGC ACTTAATTCA GGAGTCCCTC 
TCTTCCATTG ACAAGCAGTT GGCAGCTTAT ATTGCTGACA AAGTGGATGC AGCTCAGATG 
CCTCAGGAAG CCCAGAAAAT CCAATCAGAT TTGACAAGTC ATGAGATCAG TTTAGAAGAA 
ATGAAGAAAC ATAACCAGGG AAAGGAGACT GCCCAAAGGG TACTATCCCA AATTGATGTG 
GCACAGAAAA AATTGCAGGA TGTTTCCATG AAGTTTCGAT TATTCCAGAA GCCAGCCAAT 
TTTGAGCAGC GCCTACAAGA AAGTAAAATG ATTTTAGATG AAGTGAAGAT GCATTTACCT 
GCGTTGGAAA CAAAGAGTGT GGAACAGGAA GTAGTACAGT CACAGTTAAA TCATTGTGTG 
AACTTGTATA AAAGTCTGAG TGAAGTGAAG TCTGAAGTGG AAATGGTAAT AAAAACTGGA 
CGTCAGATTG TACAGAAGAA GCAGACGGAA AACCCGAAAG AGCTTGATGA AAGAGTTACA 
GCTTTGAAAT TGCATTATAA TGAGCTGGGA GCAAAGGTGA CAGAAAGAAA GCAACAGTTG 
GAAAAATGCT TGAAATTGTC CCGTAAGATG CGAAAGGAAA TGAATGCCCT GACAGAATGG 
CTGGCAGCGA CAGATATGGA ACTGACGAAG AGATCGGCAG TTGAAGGAAT GCCTAGTAAT 
TTGGATTCTG AAGTTGCCTG GGGAAAGGCT ACTCAGAAAG AGATTGAGAA ACAGAAGGTT 
CACCTAAAGA GTGTCACAGA GGTAGGAGAG GCCTTGAAAA CGGTTTTGGG CAAGAAGGAA 
ATGTTGGTGG AAGATAAACT GAGTCTTCTG AATAGTAACT GGATAGCCGT CACTTCCCGA 
GCAGAAGAGT GGTTAAACCT TTTATTGGAA TACCAGAAAC ACATGGAAAA CTTTGACCAG 
AATGTGGATT ACATCACAAA CTGGATCATT CAGGCTGATG CACTTTTGGA TGAATCTGAG 
AAAAAGAAAC CTCAGCAAAA AGAAGACATA CTTAAGCGTT TAAAGGCTGA AATGAATGAC 
ATACGTCCAA AGGTGGATTC TACACGTGAC CAAGCAGCAA ACCTGATGGC AAACCGCGGC 
GACCACTGCA GGAAAGTAGT AGAGCCCAAA ATCTCAGAGC TCAACCATCG ATTTGCAGCC 
ATTTCTCACA GAATTAAGAC TGGAAAGGCC TCCATTCCTT TGAAGGAATT GGAGCAGTTT 
AACTCAGATA TACAAAAATT GCTTGAACCA CTGGAGGCTG AAATTCAGCA GGGGGTGAAT 
CTGAAAGAGG AAGACTTCAA TAAAGATATG AGTGAAGACA ATGAGGGTAC TGTAAAAGAA 
TTGTTGCAAA GAGGAGACAA CTTACAACAA AGAATCACAG ATGAGAGAAA GCGAGAGGAA 
ATAAAGATAA AACAACAGCT GTTACAGACA AAACATAATG CTCTCAAGGA TTTGAGGTCT 
CAAAGAAGAA AAAAGGCTCT AGAAATTTCT CACCAGTGGT ATCAGTACAA GAGGCAGGCT 
GATGATCTCC TGAAATGCTT GGATGACATT GAAAAAAAAT TAGCCAGCCT ACCTGAACCC 
AGAGATGAAA GGAAAATAAA GGAAATTGAT CGTGAATTGC AGAAGAAGAA AGAGGAGCTG 
AATGCAGTGC GTAGGCAAGC TGAGGGCTTG TCTGAGGATG GGGCCGCAAT GGCAGTGGAG 
CCAACTCAGA TCCAGCTCAG CAAGCGCTGG CGGGAAATTG AGAGCAAATT TGCTCAGTTT 
CGAAGACTCA ACTTTGCACA AATTCACACT GTCCATGAAG AGTCAGTGGT GGCGATGACT 
GAAGACATGC CTTTGGAAAT TTCTTATGTG CCTTCTACTT ACCTGACTGA GATCACTCAT 
GTCTCACAAG CCCTATCAGA AGTGGAAGAG CTTCTTAATG CTCCCGACCT TTGTGCTCAA 
GATTTTGAAG ATCTCTTTAA ACAAGAGGAA TCCTTGAAGA ACATAAAAGA CAGCCTGCAA 
CAAATCTCAG GTCGGATTGA CATCATTCAC AATAAAAAGA CAGCAGCATT GCACAGTGCC 
ACTCCTGCAG AAAGGGCAAA GCTCCAGGAA GCTCTCTCAC GGCTTGATTT CCAATGGGAA 
AGAGTTAACA ATATGTACAA GGACCGACAA GGGAGATTTG ACAGATCTGT GGAAAAATGG 
CGGCGGTTTC ATTATGATAT GAAGATACTT AATCAATGGC TAACAGAAGC TGAACAGTTT 
CTCAAAAAGA CACAAATTCC TGAGAATTGG GAACATGCCA AATACAAATG GTATCTTAAG 
GAACTCCAGG ATGGCATTGG ACAGCGGCAA AGTGTTGTCA GGGTATTGAA TGCAACTGGG 
GAAGAAATAA TTCAACAGTC CTCAAAAACA GATGCCAGTA TTCTCCAAGA AAAACTGGGA 
AGCCTGAATC TGCGGTGGCA GGAGGTCTGC AAACAGCTGG CAGAAAGAAA AAAGAGGCTA 
GAGGAACAGA AGAATATCTT GTCAGAATTT CAAAGAGATG TAAATGAATT TGTTTTATGG 
TTGGAAGAAG CGGATAACGT TGCTAATATT CCACTTGAAC CTGGAAATGA GCAGCAGCTA 
AAAGAAAAAC TTGAACAAGT CAAGTTACTG GCAGAAGAGT TGCCCCTGCG CCAGGGAATT 
CTAAAACAAT TAAATGAAAC TGGAGGAACA GTGCTTGTAA GTGCTCCCCT AAGCCCAGAA 
GAGCAAGATA AACTTGAAAA TAAGCTCAAG CAGACAAATC TTCAGTGGAT AAAGGTTTCT 
AGAAATCTGC CTGAGAAGCA AGAAGAAATT GAGGCACACG TAAAAGACCT TGGACAGCTG 
GAAGAGCAGT TAAATCATCT GCTTCTATGG CTGTCTCCTA TTAGGAATCA GTTGGAAATT 
TACAATCAGC CAAATCAAAC AGGACCATTT GACATCAAGG AAATTGAAGT AGCAGTTCAA 
GCTAAACAGC CGGATGTGGA AGGGATTTTG TCTAAAGGGC AGCATTTGTA CAAGGAAAAA 
CCAGCCACTC AGCCAGCGAA GAGAAAGCTG GAAGATCTCA GCTCTGATTG GAAGGTGGTA 
ACTCAGTTGC TTCAAGAGCT GCGGGCAAAG CAGCCTGGCC CAGCTCCTGG ACTGACCACT 
GTCAGAGCCC CTCCCAGTCA GACTGTTACT CTGGTGACAC AACCCGCGGT TACCAAGGAA 
ACTGCCATCT CCAAACTAGA AATGCCATCT TCATTGCTGT TGGAGGTACC TGCACTGGCA 
GATTTCAACC GAGCTTGGAC AGAACTTACC GACTGGCTGT CTCTGCTTGA TCGAGTTATA 
AAATCACAGA GGGTGATGGT GGGTGATCTT GAAGACATTA ACGAGATGAT CATCAAGCAG 
AAGGCAACGC TGCAGGATTT GGAACAGAGG CGCCCCCAGT TGGAAGAACT CATTACCGCT 
GCCCAGAATT TGAAAAACAA GACCAGCAAT CAAGAGGCTA GAACAATCAT TACTGATCGA 
ATTGAAAGAA TTCAGAGTCA GTGGGATGAA GTACAGGAAC ATCTTCAGAA CCGGAGGCAA 
CAGTTGAATG AAATGTTAAA GGATTCCACA CAATGGCTGG AAGCTAAAGA GGAGGCTGAG 
CAGGTGTTGG GGCAGGCCAG AGCCAAGCTT GAGTCATGGA AGGAGGCTCC CTACACAGTA 
GATGCAATCC AAAAGAAAAT CACAGAAACC AAGCAGTTGG CCAAAGACCT CCGCCAGTGG 
CAGATAAATG TAGATGTTGC AAATGATTTG GCACTGAAAC TTCTCCGAGA TTATTCTGCA 
GATGATACCA GAAAAGTACA CATGATAACA GAGAACATCA ATGCCTCTTG GGCAAGCATC 
CATAAAAGAT TGAGTGAGCG AGAGGCTGCT CTGGAAGAAA CCCACAGATT ACTGCAACAG 
TTCCCCTTGG ACCTGGAGAA GTTCCTTGCC TGGCTTACAG AAGCCGAAAC AACTGCCAAC 
GTCCTGCAGG ATGCCACCCA TAAGGAAAGG CTTCTAGAAG ATTCCAAGGG AGTAAGAGAG 
CTGATGAAAC AATGGCAAGA TCTCCAAGGA GAAATCGAAG CTCACACAGA TATCTATCAC 
AACCTGGACG AAAATGGCCA AAAAGTCCTG AGATCCCTGG AAGGTTCTGA CGATGCAGCC 
TTGTTGCAAA GACGTTTGGA TAACATGAAC TTCAAGTGGA GCGAACTTCG GAAAAAGTCT 
CTCAACATTA GGTCTCACTT GGAAGCCAGT TCTGACCAGT GGAAGCGTCT GCACCTTTCT 
CTTCAGGAAC TTCTGGTATG GCTCCAGCTG AAAGATGATG AGTTAAGCCG GCAGGCACCC 
ATTGGAGGAG ACTTTCCAGC GGTGCAGAAG CAGAATGATG TACACAGGGC CTTCAAGAGG 
GAATTGAAAA CGAAAGAACC TGTAATCATG AGTACTCTTG AGACTGTACG AATATTTCTG 
ACAGAGCAGC CTTTAGAAGG ACTAGAGAAA CTCTACCAGG AGCCCAGAGA GCTGCCTCCT 
GAAGAGAGAG CCCAGAATGT CACACGGCTC CTACGAAAGC AAGCTGAGGA GGTCAACACT 
CAGTGGGAAA AACTGAACGT GCACTCTGCA GACTGGCAGA GAAAAATAGA CGAGGCCCTC 
GAAAGACTCC AGGAGCTTCA GGAAGCAACA GATGAGCTGG ATCTCAAACT ACGTCAGGCA 
GAGGTGATCA AAGGATCCTG GCAGCCTGTG GGTGATCTCC TCATTGACTC TCTCCAAGAT 
CACCTCGAAA AAGTCAAGGC GCTTCGAGGA GAAATTACAC CTCTGAAAGA GAATGTCAGC 
TACGTCAATG ACCTTGCTCG CCAACTCACT ACGTTGGGCA TTCAGCTGTC ACCATATAAC 
CTCAACACTC TGGAAGACCT GAACACCAGA TGGAAGCTTC TGCAGGTGGC CATTGAGGAC 
CGCATCAGGC AGCTGCATGA AGCGCACAGG GACTTTGGAC CAGCCTCCCA GCACTTCCTT 
TCCACTTCTG TCCAGGGTCC CTGGGAGAGA GCCATCTCAC CAAACAAAGT GCCCTACTAT 
ATCAACCACG AGACCCAAAC AACTTGCTGG GACCATCCCA AAATGACAGA GCTCTACCAG 
TCTTTAGCTG ACCTGAATAA TGTCAGGTTC TCAGCTTACA GGACTGCCAT GAAACTCCGA 
AGACTGCAGA AGGCCCTTTG CTTGGATCTC TTGAGCCTAT CGGCTGCATG CGATGCCTTG 
GACCAGCACA ACCTCAAGCA AAATGACCAG CCCATGGATA TCCTGCAGGT CATTAACTGT 
CTGACCACTA TTTATGATCG CCTAGAGCAA GAGCACAACA ATCTGGTCAA CGTCCCTCTC 
TGCGTGGATA TGTGTCTCAA TTGGCTGCTG AATGTTTATG ACACGGGACG AACGGGGAGG 
ATCCGGGTCC TGTCTTTTAA AACTGGCATC ATTTCTCTGT GTAAAGCCCA TTTGGAAGAC 
AAGTACAGAT ACCTCTTCAA GCAAGTGGCA AGTTCGACAG GATTTTGTGA CCAGCGCAGG 
CTGGGCCTCC TCCTGCATGA CTCTATCCAG ATCCCAAGAC AGTTGGGTGA AGTCGCATCG 
TTCGGGGGCA GTAACATTGA GCCGAGTGTC AGGAGCTGCT TCCAGTTTGC TAATAATAAG 
CCTGAGATCG AAGCGGCCCT CTTCCTAGAC TGGATGCGCC TGGAGCCCCA GTCCATGGTG 
TGGCTGCCTG TCCTGCACCG AGTGGCTGCC GCGGAAACTG CCAAGCACCA GGCCAAGTGC 
AACATCTGCA AGGAGTGTCC CATCATCGGA TTCAGGTACA GGAGTCTAAA GCACTTTAAT 
TATGACATCT GCCAAAGTTG CTTTTTTTCT GGTCGAGTTG CAAAAGGCCA TAAAATGCAC 
TATCCCATGG TGGAATACTG CACTCCGACT ACATCGGGAG AAGATGTCCG TGACTTTGCC 
AAGGTACTAA AAAACAAATT TCGAACCAAA AGGTATTTTG CGAAGCATCC CCGAATGGGC 
TACCTGCCAG TGCAGACTGT CTTAGAGGGG GACAACATGG AAACTCCTGT CACTCTGATC 
AACTTCTGGC CGGTAGATTC TGCGCCTGCC TCGTCCCCTC AGCTTTCACA CGATGATACT 
CATTCACGCA TTGAGCATTA TGCTAGCAGG CTAGCAGAAA TGGAAAACAG CAATGGATCT 
TATCTAAATG ATAGCATCTC TCCTAATGAG AGCATAGATG ATGAACATTT GTTAATCCAG 
CATTACTGCC AAAGTTTGAA CCAGGACTCC CCCCTGAGCC AGCCTCGTAG TCCTGCCCAG 
ATCTTGATTT CCTTAGAGAG TGAGGAAAGA GGGGAGCTAG AGAGAATCCT AGCAGATCTT 
GAGGAAGAAA ACAGAAATCT GCAAGCAGAG TATGATCGTC TAAAGCAGCA GCATGAACAC 
AAAGGCCTGT CCCCACTGCC ATCCCCTCCT GAAATGATGC CTACTTCTCC CCAAAGTCCC 
CGGGATGCTG AGCTCATCGC TGAGGCCAAG CTGCTGCGTC AACACAAAGG CCGCCTGGAA 
GCCAGGATGC AAATCCTTGA AGACCATAAC AAACAACTGG AATCCCAGTT ACACAGGCTC 
AGGCAGCTGC TGGAGCAACC CCAGGCAGAG GCCAAGGTGA ATGGTACAAC GGTGTCTTCT 
CCTTCTACCT CTCTTCAGAG GTCAGATAGC AGTCAGCCTA TGCTGCTCCG GGTGGTCGGC 
AGTCAGACTT CAGAATCCAT GGGCGAGGAA GACCTGCTCA GCCCTCCCCA GGACACAAGC 
ACAGGGTTAG AGGAAGTGAT GGAGCAGCTC AACCACTCCT TCCCTAGTTC CAGAGGACAC 
AATGTAGGAA GCCTTTTCCA CTTGGCAGAT GATTTGGGCA GAGCGATGGA GTCCTTAGTT 
TCAGTCATGA CAGATGAAGA AGGGGCAGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013966819.1
CDS	183..11255
Translation	MEDEREDVQKKTFTKWINAQFSKFGKQHIENLFSDLQDGRRLLDLLEGLTGQKLPKEKGSTRVHALNNVNKALRVLQKNNVDLVNIGSTDIVDGNHKLTLGLIWNIILHWQVKNVMKNIMAGLQQTNSEKILLSWVRQSTRNYPQVNVINFTTSWSDGLALNALIHSHRPDLFDWNSVVCQQSATQRLEHAFNIAKYQLGIEKLLDPEDVATTYPDKKSILMYITSLFQVLPQQVSIEAIQEVEMLPRPSKVTREEHFQLHHQMHYSQQITVSLAQGYERAPSSPKPRFKSYAYTQAAYVTTSDPTRSPLPSQHLETPEDKSFGRSLTETEANLDSYQTALEEVLSWLLSAEDALQAQGEISNDVEEVKEQFHTHEGYMMDLTSHQGRVGNVLQLGSQLIGTGKLSEDEETEVQEQMNLLNSRWECLRVASMEKQSNLHKVLMDLQNQQLKELNDWLTKTEERTRKMEKEPLGPDIEDLKRQVQQHKVLQEDLEQEQVRVNSLTHMVVVVDESSGDHATAALEEQLKVLGDRWANICRWTEDRWVLLQDILLKWQRFTEEQCLFSAWLSEKEDAVNKIHTTGFKDQSEVLSNLQKLAVLKTDLEKKKQTMDKLCSLNQDLLSALKNTVVAHKMEAWLDNFAQRWDNLVQKLEKSSAQISQAVTTTQPSLTQTTVMETVTMVTTREHILVKHAQEELPPPPPQKKRQIIVDSEIRKRLDVDITELHSWITRSEAVLQSPEFAIYRKEGNFSDLKEKVNAIEREKAEKFRKLQDASRSAQALVEQMVNEGVNADSIKQASEQLNSRWIEFCQLLSERLNWLEYQNNIITFYNQLQQLEQMTTTAENWLKTQPTTTSEPTAIKSQLKICKDEINRLSALQPQIERLKIQSIALKEKGQGPMFLDADFVAFTNHFNQVFADVQAREKELQTIFDSLPPMRYQETMSTILTWIQQSETKLSIPQVTVTEYDIMEQRLGELQALQSSLQEQQNGLNYLSTTVKEMSKKAPLSDISRKYQSEFEEIEGRWKKLSSQLVEHCQKLEEQMAKLRKIQNHIKTLKKWITEVDVFLKEEWPALGDSEILKRQLKQCRLLVNDIQTIQPSLNSVNEGAQKMKNEAEPEFAGRLETELRELNTQWDYMCRQVYARKEALKGGLDKTVSLQKDLSEMHEWMTQAEEEYLERDFEYKTPDELQTAVEEMKRAKEEAQQKEAKVKLLTESVNSVIAQAPPAAQEALKKELDTLTTNYQWLCTRLNGKCKTLEEVWACWHELLSYLEKANKWLSEVEVKLKTTENISGGAEEIAEVLDSLENLMQHSEDNPNQIRILAQTLTDGGVMDELINEELETFNSRWRELHEEAVRRQKLLEQSIQSAQEIEKSLHLIQESLSSIDKQLAAYIADKVDAAQMPQEAQKIQSDLTSHEISLEEMKKHNQGKETAQRVLSQIDVAQKKLQDVSMKFRLFQKPANFEQRLQESKMILDEVKMHLPALETKSVEQEVVQSQLNHCVNLYKSLSEVKSEVEMVIKTGRQIVQKKQTENPKELDERVTALKLHYNELGAKVTERKQQLEKCLKLSRKMRKEMNALTEWLAATDMELTKRSAVEGMPSNLDSEVAWGKATQKEIEKQKVHLKSVTEVGEALKTVLGKKEMLVEDKLSLLNSNWIAVTSRAEEWLNLLLEYQKHMENFDQNVDYITNWIIQADALLDESEKKKPQQKEDILKRLKAEMNDIRPKVDSTRDQAANLMANRGDHCRKVVEPKISELNHRFAAISHRIKTGKASIPLKELEQFNSDIQKLLEPLEAEIQQGVNLKEEDFNKDMSEDNEGTVKELLQRGDNLQQRITDERKREEIKIKQQLLQTKHNALKDLRSQRRKKALEISHQWYQYKRQADDLLKCLDDIEKKLASLPEPRDERKIKEIDRELQKKKEELNAVRRQAEGLSEDGAAMAVEPTQIQLSKRWREIESKFAQFRRLNFAQIHTVHEESVVAMTEDMPLEISYVPSTYLTEITHVSQALSEVEELLNAPDLCAQDFEDLFKQEESLKNIKDSLQQISGRIDIIHNKKTAALHSATPAERAKLQEALSRLDFQWERVNNMYKDRQGRFDRSVEKWRRFHYDMKILNQWLTEAEQFLKKTQIPENWEHAKYKWYLKELQDGIGQRQSVVRVLNATGEEIIQQSSKTDASILQEKLGSLNLRWQEVCKQLAERKKRLEEQKNILSEFQRDVNEFVLWLEEADNVANIPLEPGNEQQLKEKLEQVKLLAEELPLRQGILKQLNETGGTVLVSAPLSPEEQDKLENKLKQTNLQWIKVSRNLPEKQEEIEAHVKDLGQLEEQLNHLLLWLSPIRNQLEIYNQPNQTGPFDIKEIEVAVQAKQPDVEGILSKGQHLYKEKPATQPAKRKLEDLSSDWKVVTQLLQELRAKQPGPAPGLTTVRAPPSQTVTLVTQPAVTKETAISKLEMPSSLLLEVPALADFNRAWTELTDWLSLLDRVIKSQRVMVGDLEDINEMIIKQKATLQDLEQRRPQLEELITAAQNLKNKTSNQEARTIITDRIERIQSQWDEVQEHLQNRRQQLNEMLKDSTQWLEAKEEAEQVLGQARAKLESWKEAPYTVDAIQKKITETKQLAKDLRQWQINVDVANDLALKLLRDYSADDTRKVHMITENINASWASIHKRLSEREAALEETHRLLQQFPLDLEKFLAWLTEAETTANVLQDATHKERLLEDSKGVRELMKQWQDLQGEIEAHTDIYHNLDENGQKVLRSLEGSDDAALLQRRLDNMNFKWSELRKKSLNIRSHLEASSDQWKRLHLSLQELLVWLQLKDDELSRQAPIGGDFPAVQKQNDVHRAFKRELKTKEPVIMSTLETVRIFLTEQPLEGLEKLYQEPRELPPEERAQNVTRLLRKQAEEVNTQWEKLNVHSADWQRKIDEALERLQELQEATDELDLKLRQAEVIKGSWQPVGDLLIDSLQDHLEKVKALRGEITPLKENVSYVNDLARQLTTLGIQLSPYNLNTLEDLNTRWKLLQVAIEDRIRQLHEAHRDFGPASQHFLSTSVQGPWERAISPNKVPYYINHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQVINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPVTLINFWPVDSAPASSPQLSHDDTHSRIEHYASRLAEMENSNGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKQQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDTSTGLEEVMEQLNHSFPSSRGHNVGSLFHLADDLGRAMESLVSVMTDEEGAE

Target ORF information:

RefSeq Version	XM_014111344.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris dystrophin (DMD), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111344.2

ORF Insert Sequence:

ATGGAAGATG AAAGAGAAGA TGTTCAAAAG AAAACATTCA CAAAATGGAT AAATGCACAG 
TTTTCTAAGT TTGGGAAGCA GCACATAGAG AACCTCTTCA GTGACCTACA GGATGGGAGA 
CGCCTCCTAG ACCTTTTGGA AGGCCTGACA GGGCAAAAAC TGCCAAAAGA AAAAGGATCC 
ACAAGAGTTC ATGCCCTGAA CAATGTCAAC AAGGCACTGC GCGTCTTGCA GAAAAATAAT 
GTTGATTTAG TGAACATTGG AAGTACTGAC ATAGTAGATG GAAATCACAA ACTGACTCTT 
GGTTTGATTT GGAATATAAT CCTCCACTGG CAGGTCAAAA ATGTAATGAA AAATATCATG 
GCTGGATTGC AACAAACCAA CAGTGAAAAG ATTCTCCTGA GCTGGGTCCG ACAATCAACT 
CGTAATTATC CACAGGTTAA TGTCATTAAC TTCACCACCA GCTGGTCTGA TGGCCTGGCT 
TTGAACGCTC TCATCCACAG TCATAGGCCA GACCTGTTTG ATTGGAATAG TGTGGTTTGC 
CAGCAGTCAG CCACACAACG CCTGGAACAT GCATTCAACA TTGCCAAATA TCAATTAGGC 
ATAGAGAAAC TGCTTGATCC TGAAGATGTT GCCACCACTT ATCCAGATAA GAAGTCCATC 
TTAATGTATA TCACATCACT CTTCCAAGTT TTGCCTCAAC AAGTGAGCAT TGAAGCCATC 
CAGGAAGTGG AAATGTTGCC AAGGCCATCA AAAGTTACTA GAGAAGAACA TTTTCAATTA 
CATCATCAAA TGCACTATTC TCAACAGATC ACAGTCAGTC TAGCACAGGG CTATGAACGA 
GCCCCTTCCT CTCCTAAGCC TCGGTTCAAG AGCTATGCCT ACACACAGGC TGCTTATGTC 
ACCACTTCTG ACCCCACACG GAGCCCACTT CCTTCACAGC ATTTGGAAAC TCCTGAAGAC 
AAGTCATTTG GCCGGTCATT GACAGAGACC GAAGCAAACC TGGACAGTTA TCAAACAGCT 
TTGGAAGAAG TACTCTCGTG GCTTCTTTCA GCTGAGGATG CACTGCAAGC CCAAGGAGAG 
ATTTCTAATG ATGTCGAAGA AGTGAAAGAA CAATTTCATA CTCATGAGGG ATATATGATG 
GACTTGACAT CCCATCAGGG ACGGGTCGGT AATGTTCTCC AACTGGGAAG TCAACTGATT 
GGAACAGGGA AATTATCAGA AGATGAAGAA ACCGAAGTGC AGGAACAAAT GAATCTCCTC 
AATTCAAGAT GGGAATGCCT CAGGGTAGCT AGCATGGAAA AACAAAGCAA TTTACATAAA 
GTTCTAATGG ATCTCCAGAA TCAGCAACTG AAAGAGTTAA ATGACTGGCT AACCAAAACA 
GAAGAGAGAA CAAGGAAAAT GGAGAAGGAG CCCCTTGGAC CTGATATTGA AGACCTAAAA 
CGCCAAGTAC AACAACATAA GGTGCTTCAA GAAGACTTAG AACAGGAACA AGTCAGGGTC 
AATTCCCTCA CTCATATGGT GGTGGTAGTC GATGAATCTA GTGGAGACCA TGCAACTGCT 
GCTTTGGAAG AACAACTTAA GGTACTGGGA GATCGATGGG CAAACATCTG TAGGTGGACA 
GAAGATCGCT GGGTTCTTTT ACAAGACATC CTCCTAAAAT GGCAGCGTTT TACTGAAGAA 
CAGTGCCTTT TCAGTGCATG GCTTTCGGAG AAGGAAGATG CAGTGAACAA GATTCACACA 
ACTGGCTTTA AGGATCAAAG TGAAGTGTTA TCAAATCTTC AGAAACTGGC TGTCTTAAAA 
ACAGATCTGG AAAAGAAGAA GCAAACCATG GACAAACTCT GCTCACTCAA CCAAGACCTT 
CTTTCAGCGC TGAAAAACAC AGTGGTAGCC CACAAGATGG AAGCATGGCT GGACAACTTT 
GCCCAGCGCT GGGATAATTT AGTCCAGAAA CTTGAAAAAA GTTCAGCACA GATTTCACAG 
GCTGTCACCA CCACTCAGCC ATCACTAACA CAGACAACTG TAATGGAAAC AGTAACTATG 
GTGACCACGA GGGAACACAT CTTGGTAAAG CATGCCCAAG AGGAACTGCC ACCACCACCC 
CCTCAGAAGA AGAGGCAGAT TATCGTGGAT TCTGAAATTA GGAAAAGGTT GGATGTCGAT 
ATAACTGAAC TTCACAGTTG GATTACTCGT TCAGAAGCTG TGTTGCAGAG TCCTGAATTT 
GCAATCTATC GGAAGGAAGG CAACTTCTCA GACCTTAAAG AAAAAGTCAA TGCCATAGAG 
CGAGAAAAAG CCGAGAAGTT CAGAAAACTG CAAGATGCCA GCAGATCAGC TCAGGCCCTG 
GTGGAACAGA TGGTGAATGA GGGTGTTAAT GCTGACAGCA TCAAACAAGC CTCCGAACAA 
CTGAACAGCC GGTGGATAGA GTTCTGCCAA TTGCTAAGCG AGAGACTTAA CTGGCTGGAG 
TATCAGAACA ACATCATCAC TTTCTATAAT CAGCTACAAC AATTGGAGCA GATGACAACT 
ACTGCTGAAA ACTGGTTGAA AACCCAGCCT ACCACCACAT CAGAGCCAAC AGCAATTAAA 
AGCCAGTTAA AAATTTGTAA GGATGAAATC AACCGACTGT CAGCTCTTCA GCCTCAAATC 
GAGCGATTAA AAATTCAAAG CATAGCCCTG AAAGAGAAAG GACAAGGGCC AATGTTCCTG 
GATGCAGACT TTGTGGCCTT TACAAATCAT TTTAACCAAG TCTTTGCTGA TGTGCAGGCA 
AGAGAAAAAG AGCTACAAAC AATTTTTGAC AGTTTGCCAC CCATGCGCTA TCAGGAGACT 
ATGAGTACCA TCCTGACATG GATCCAGCAG TCAGAAACCA AACTCTCTAT ACCTCAGGTT 
ACTGTCACTG AATATGACAT CATGGAACAG AGACTCGGAG AGCTACAGGC TTTACAAAGC 
TCTCTGCAAG AGCAACAAAA TGGCCTAAAC TATCTCAGCA CCACTGTGAA AGAGATGTCA 
AAGAAAGCAC CACTGTCTGA TATTAGTCGG AAATATCAAT CAGAATTTGA AGAGATTGAG 
GGACGTTGGA AGAAGCTGTC TTCCCAGCTG GTTGAACATT GTCAAAAGTT GGAGGAGCAA 
ATGGCTAAAC TTCGAAAAAT TCAGAATCAC ATAAAAACTC TGAAGAAATG GATCACTGAA 
GTCGATGTTT TCCTGAAGGA GGAATGGCCT GCCCTTGGGG ATTCAGAAAT TCTGAAAAGA 
CAGCTGAAAC AGTGCAGGCT TTTAGTCAAT GACATTCAGA CCATCCAGCC TAGTCTCAAC 
AGTGTCAATG AAGGGGCTCA GAAGATGAAG AATGAAGCAG AACCAGAGTT TGCTGGCAGA 
CTTGAGACAG AGCTCCGAGA ACTTAACACC CAGTGGGATT ACATGTGCCG CCAGGTCTAT 
GCCAGGAAGG AAGCCTTAAA AGGAGGTTTG GATAAAACTG TAAGTCTTCA GAAAGATCTG 
TCAGAGATGC ATGAGTGGAT GACACAAGCT GAAGAAGAAT ACCTAGAGAG AGATTTCGAA 
TACAAGACCC CTGATGAATT ACAGACAGCA GTTGAAGAGA TGAAGAGAGC TAAAGAAGAG 
GCCCAGCAAA AAGAAGCAAA AGTGAAACTC CTAACCGAGT CCGTCAATAG TGTCATAGCT 
CAGGCTCCAC CTGCAGCACA AGAGGCCTTA AAAAAGGAAC TTGACACTCT CACCACCAAC 
TACCAGTGGC TCTGCACCAG GCTCAATGGC AAATGCAAGA CCTTGGAAGA AGTTTGGGCG 
TGCTGGCATG AGTTATTGTC CTACTTGGAG AAGGCAAACA AGTGGCTAAG TGAAGTAGAA 
GTCAAGCTTA AAACCACTGA AAATATTTCT GGGGGAGCTG AGGAAATCGC CGAGGTGCTT 
GATTCGCTTG AAAATTTGAT GCAACATTCA GAGGATAACC CGAATCAGAT TCGCATATTG 
GCACAGACCT TGACAGATGG TGGAGTCATG GATGAACTGA TCAATGAGGA GCTTGAGACA 
TTTAATTCTC GTTGGAGAGA ACTCCATGAA GAGGCTGTGA GGAGGCAAAA GTTGCTTGAG 
CAGAGTATCC AGTCGGCCCA GGAGATAGAA AAATCCTTGC ACTTAATTCA GGAGTCCCTC 
TCTTCCATTG ACAAGCAGTT GGCAGCTTAT ATTGCTGACA AAGTGGATGC AGCTCAGATG 
CCTCAGGAAG CCCAGAAAAT CCAATCAGAT TTGACAAGTC ATGAGATCAG TTTAGAAGAA 
ATGAAGAAAC ATAACCAGGG AAAGGAGACT GCCCAAAGGG TACTATCCCA AATTGATGTG 
GCACAGAAAA AATTGCAGGA TGTTTCCATG AAGTTTCGAT TATTCCAGAA GCCAGCCAAT 
TTTGAGCAGC GCCTACAAGA AAGTAAAATG ATTTTAGATG AAGTGAAGAT GCATTTACCT 
GCGTTGGAAA CAAAGAGTGT GGAACAGGAA GTAGTACAGT CACAGTTAAA TCATTGTGTG 
AACTTGTATA AAAGTCTGAG TGAAGTGAAG TCTGAAGTGG AAATGGTAAT AAAAACTGGA 
CGTCAGATTG TACAGAAGAA GCAGACGGAA AACCCGAAAG AGCTTGATGA AAGAGTTACA 
GCTTTGAAAT TGCATTATAA TGAGCTGGGA GCAAAGGTGA CAGAAAGAAA GCAACAGTTG 
GAAAAATGCT TGAAATTGTC CCGTAAGATG CGAAAGGAAA TGAATGCCCT GACAGAATGG 
CTGGCAGCGA CAGATATGGA ACTGACGAAG AGATCGGCAG TTGAAGGAAT GCCTAGTAAT 
TTGGATTCTG AAGTTGCCTG GGGAAAGGCT ACTCAGAAAG AGATTGAGAA ACAGAAGGTT 
CACCTAAAGA GTGTCACAGA GGTAGGAGAG GCCTTGAAAA CGGTTTTGGG CAAGAAGGAA 
ATGTTGGTGG AAGATAAACT GAGTCTTCTG AATAGTAACT GGATAGCCGT CACTTCCCGA 
GCAGAAGAGT GGTTAAACCT TTTATTGGAA TACCAGAAAC ACATGGAAAA CTTTGACCAG 
AATGTGGATT ACATCACAAA CTGGATCATT CAGGCTGATG CACTTTTGGA TGAATCTGAG 
AAAAAGAAAC CTCAGCAAAA AGAAGACATA CTTAAGCGTT TAAAGGCTGA AATGAATGAC 
ATACGTCCAA AGGTGGATTC TACACGTGAC CAAGCAGCAA ACCTGATGGC AAACCGCGGC 
GACCACTGCA GGAAAGTAGT AGAGCCCAAA ATCTCAGAGC TCAACCATCG ATTTGCAGCC 
ATTTCTCACA GAATTAAGAC TGGAAAGGCC TCCATTCCTT TGAAGGAATT GGAGCAGTTT 
AACTCAGATA TACAAAAATT GCTTGAACCA CTGGAGGCTG AAATTCAGCA GGGGGTGAAT 
CTGAAAGAGG AAGACTTCAA TAAAGATATG AGTGAAGACA ATGAGGGTAC TGTAAAAGAA 
TTGTTGCAAA GAGGAGACAA CTTACAACAA AGAATCACAG ATGAGAGAAA GCGAGAGGAA 
ATAAAGATAA AACAACAGCT GTTACAGACA AAACATAATG CTCTCAAGGA TTTGAGGTCT 
CAAAGAAGAA AAAAGGCTCT AGAAATTTCT CACCAGTGGT ATCAGTACAA GAGGCAGGCT 
GATGATCTCC TGAAATGCTT GGATGACATT GAAAAAAAAT TAGCCAGCCT ACCTGAACCC 
AGAGATGAAA GGAAAATAAA GGAAATTGAT CGTGAATTGC AGAAGAAGAA AGAGGAGCTG 
AATGCAGTGC GTAGGCAAGC TGAGGGCTTG TCTGAGGATG GGGCCGCAAT GGCAGTGGAG 
CCAACTCAGA TCCAGCTCAG CAAGCGCTGG CGGGAAATTG AGAGCAAATT TGCTCAGTTT 
CGAAGACTCA ACTTTGCACA AATTCACACT GTCCATGAAG AGTCAGTGGT GGCGATGACT 
GAAGACATGC CTTTGGAAAT TTCTTATGTG CCTTCTACTT ACCTGACTGA GATCACTCAT 
GTCTCACAAG CCCTATCAGA AGTGGAAGAG CTTCTTAATG CTCCCGACCT TTGTGCTCAA 
GATTTTGAAG ATCTCTTTAA ACAAGAGGAA TCCTTGAAGA ACATAAAAGA CAGCCTGCAA 
CAAATCTCAG GTCGGATTGA CATCATTCAC AATAAAAAGA CAGCAGCATT GCACAGTGCC 
ACTCCTGCAG AAAGGGCAAA GCTCCAGGAA GCTCTCTCAC GGCTTGATTT CCAATGGGAA 
AGAGTTAACA ATATGTACAA GGACCGACAA GGGAGATTTG ACAGATCTGT GGAAAAATGG 
CGGCGGTTTC ATTATGATAT GAAGATACTT AATCAATGGC TAACAGAAGC TGAACAGTTT 
CTCAAAAAGA CACAAATTCC TGAGAATTGG GAACATGCCA AATACAAATG GTATCTTAAG 
GAACTCCAGG ATGGCATTGG ACAGCGGCAA AGTGTTGTCA GGGTATTGAA TGCAACTGGG 
GAAGAAATAA TTCAACAGTC CTCAAAAACA GATGCCAGTA TTCTCCAAGA AAAACTGGGA 
AGCCTGAATC TGCGGTGGCA GGAGGTCTGC AAACAGCTGG CAGAAAGAAA AAAGAGGCTA 
GAGGAACAGA AGAATATCTT GTCAGAATTT CAAAGAGATG TAAATGAATT TGTTTTATGG 
TTGGAAGAAG CGGATAACGT TGCTAATATT CCACTTGAAC CTGGAAATGA GCAGCAGCTA 
AAAGAAAAAC TTGAACAAGT CAAGTTACTG GCAGAAGAGT TGCCCCTGCG CCAGGGAATT 
CTAAAACAAT TAAATGAAAC TGGAGGAACA GTGCTTGTAA GTGCTCCCCT AAGCCCAGAA 
GAGCAAGATA AACTTGAAAA TAAGCTCAAG CAGACAAATC TTCAGTGGAT AAAGGTTTCT 
AGAAATCTGC CTGAGAAGCA AGAAGAAATT GAGGCACACG TAAAAGACCT TGGACAGCTG 
GAAGAGCAGT TAAATCATCT GCTTCTATGG CTGTCTCCTA TTAGGAATCA GTTGGAAATT 
TACAATCAGC CAAATCAAAC AGGACCATTT GACATCAAGG AAATTGAAGT AGCAGTTCAA 
GCTAAACAGC CGGATGTGGA AGGGATTTTG TCTAAAGGGC AGCATTTGTA CAAGGAAAAA 
CCAGCCACTC AGCCAGCGAA GAGAAAGCTG GAAGATCTCA GCTCTGATTG GAAGGTGGTA 
ACTCAGTTGC TTCAAGAGCT GCGGGCAAAG CAGCCTGGCC CAGCTCCTGG ACTGACCACT 
GTCAGAGCCC CTCCCAGTCA GACTGTTACT CTGGTGACAC AACCCGCGGT TACCAAGGAA 
ACTGCCATCT CCAAACTAGA AATGCCATCT TCATTGCTGT TGGAGGTACC TGCACTGGCA 
GATTTCAACC GAGCTTGGAC AGAACTTACC GACTGGCTGT CTCTGCTTGA TCGAGTTATA 
AAATCACAGA GGGTGATGGT GGGTGATCTT GAAGACATTA ACGAGATGAT CATCAAGCAG 
AAGGCAACGC TGCAGGATTT GGAACAGAGG CGCCCCCAGT TGGAAGAACT CATTACCGCT 
GCCCAGAATT TGAAAAACAA GACCAGCAAT CAAGAGGCTA GAACAATCAT TACTGATCGA 
ATTGAAAGAA TTCAGAGTCA GTGGGATGAA GTACAGGAAC ATCTTCAGAA CCGGAGGCAA 
CAGTTGAATG AAATGTTAAA GGATTCCACA CAATGGCTGG AAGCTAAAGA GGAGGCTGAG 
CAGGTGTTGG GGCAGGCCAG AGCCAAGCTT GAGTCATGGA AGGAGGCTCC CTACACAGTA 
GATGCAATCC AAAAGAAAAT CACAGAAACC AAGCAGTTGG CCAAAGACCT CCGCCAGTGG 
CAGATAAATG TAGATGTTGC AAATGATTTG GCACTGAAAC TTCTCCGAGA TTATTCTGCA 
GATGATACCA GAAAAGTACA CATGATAACA GAGAACATCA ATGCCTCTTG GGCAAGCATC 
CATAAAAGAT TGAGTGAGCG AGAGGCTGCT CTGGAAGAAA CCCACAGATT ACTGCAACAG 
TTCCCCTTGG ACCTGGAGAA GTTCCTTGCC TGGCTTACAG AAGCCGAAAC AACTGCCAAC 
GTCCTGCAGG ATGCCACCCA TAAGGAAAGG CTTCTAGAAG ATTCCAAGGG AGTAAGAGAG 
CTGATGAAAC AATGGCAAGA TCTCCAAGGA GAAATCGAAG CTCACACAGA TATCTATCAC 
AACCTGGACG AAAATGGCCA AAAAGTCCTG AGATCCCTGG AAGGTTCTGA CGATGCAGCC 
TTGTTGCAAA GACGTTTGGA TAACATGAAC TTCAAGTGGA GCGAACTTCG GAAAAAGTCT 
CTCAACATTA GGTCTCACTT GGAAGCCAGT TCTGACCAGT GGAAGCGTCT GCACCTTTCT 
CTTCAGGAAC TTCTGGTATG GCTCCAGCTG AAAGATGATG AGTTAAGCCG GCAGGCACCC 
ATTGGAGGAG ACTTTCCAGC GGTGCAGAAG CAGAATGATG TACACAGGGC CTTCAAGAGG 
GAATTGAAAA CGAAAGAACC TGTAATCATG AGTACTCTTG AGACTGTACG AATATTTCTG 
ACAGAGCAGC CTTTAGAAGG ACTAGAGAAA CTCTACCAGG AGCCCAGAGA GCTGCCTCCT 
GAAGAGAGAG CCCAGAATGT CACACGGCTC CTACGAAAGC AAGCTGAGGA GGTCAACACT 
CAGTGGGAAA AACTGAACGT GCACTCTGCA GACTGGCAGA GAAAAATAGA CGAGGCCCTC 
GAAAGACTCC AGGAGCTTCA GGAAGCAACA GATGAGCTGG ATCTCAAACT ACGTCAGGCA 
GAGGTGATCA AAGGATCCTG GCAGCCTGTG GGTGATCTCC TCATTGACTC TCTCCAAGAT 
CACCTCGAAA AAGTCAAGGC GCTTCGAGGA GAAATTACAC CTCTGAAAGA GAATGTCAGC 
TACGTCAATG ACCTTGCTCG CCAACTCACT ACGTTGGGCA TTCAGCTGTC ACCATATAAC 
CTCAACACTC TGGAAGACCT GAACACCAGA TGGAAGCTTC TGCAGGTGGC CATTGAGGAC 
CGCATCAGGC AGCTGCATGA AGCGCACAGG GACTTTGGAC CAGCCTCCCA GCACTTCCTT 
TCCACTTCTG TCCAGGGTCC CTGGGAGAGA GCCATCTCAC CAAACAAAGT GCCCTACTAT 
ATCAACCACG AGACCCAAAC AACTTGCTGG GACCATCCCA AAATGACAGA GCTCTACCAG 
TCTTTAGCTG ACCTGAATAA TGTCAGGTTC TCAGCTTACA GGACTGCCAT GAAACTCCGA 
AGACTGCAGA AGGCCCTTTG CTTGGATCTC TTGAGCCTAT CGGCTGCATG CGATGCCTTG 
GACCAGCACA ACCTCAAGCA AAATGACCAG CCCATGGATA TCCTGCAGGT CATTAACTGT 
CTGACCACTA TTTATGATCG CCTAGAGCAA GAGCACAACA ATCTGGTCAA CGTCCCTCTC 
TGCGTGGATA TGTGTCTCAA TTGGCTGCTG AATGTTTATG ACACGGGACG AACGGGGAGG 
ATCCGGGTCC TGTCTTTTAA AACTGGCATC ATTTCTCTGT GTAAAGCCCA TTTGGAAGAC 
AAGTACAGAT ACCTCTTCAA GCAAGTGGCA AGTTCGACAG GATTTTGTGA CCAGCGCAGG 
CTGGGCCTCC TCCTGCATGA CTCTATCCAG ATCCCAAGAC AGTTGGGTGA AGTCGCATCG 
TTCGGGGGCA GTAACATTGA GCCGAGTGTC AGGAGCTGCT TCCAGTTTGC TAATAATAAG 
CCTGAGATCG AAGCGGCCCT CTTCCTAGAC TGGATGCGCC TGGAGCCCCA GTCCATGGTG 
TGGCTGCCTG TCCTGCACCG AGTGGCTGCC GCGGAAACTG CCAAGCACCA GGCCAAGTGC 
AACATCTGCA AGGAGTGTCC CATCATCGGA TTCAGGTACA GGAGTCTAAA GCACTTTAAT 
TATGACATCT GCCAAAGTTG CTTTTTTTCT GGTCGAGTTG CAAAAGGCCA TAAAATGCAC 
TATCCCATGG TGGAATACTG CACTCCGACT ACATCGGGAG AAGATGTCCG TGACTTTGCC 
AAGGTACTAA AAAACAAATT TCGAACCAAA AGGTATTTTG CGAAGCATCC CCGAATGGGC 
TACCTGCCAG TGCAGACTGT CTTAGAGGGG GACAACATGG AAACTCCTGT CACTCTGATC 
AACTTCTGGC CGGTAGATTC TGCGCCTGCC TCGTCCCCTC AGCTTTCACA CGATGATACT 
CATTCACGCA TTGAGCATTA TGCTAGCAGG CTAGCAGAAA TGGAAAACAG CAATGGATCT 
TATCTAAATG ATAGCATCTC TCCTAATGAG AGCATAGATG ATGAACATTT GTTAATCCAG 
CATTACTGCC AAAGTTTGAA CCAGGACTCC CCCCTGAGCC AGCCTCGTAG TCCTGCCCAG 
ATCTTGATTT CCTTAGAGAG TGAGGAAAGA GGGGAGCTAG AGAGAATCCT AGCAGATCTT 
GAGGAAGAAA ACAGAAATCT GCAAGCAGAG TATGATCGTC TAAAGCAGCA GCATGAACAC 
AAAGGCCTGT CCCCACTGCC ATCCCCTCCT GAAATGATGC CTACTTCTCC CCAAAGTCCC 
CGGGATGCTG AGCTCATCGC TGAGGCCAAG CTGCTGCGTC AACACAAAGG CCGCCTGGAA 
GCCAGGATGC AAATCCTTGA AGACCATAAC AAACAACTGG AATCCCAGTT ACACAGGCTC 
AGGCAGCTGC TGGAGCAACC CCAGGCAGAG GCCAAGGTGA ATGGTACAAC GGTGTCTTCT 
CCTTCTACCT CTCTTCAGAG GTCAGATAGC AGTCAGCCTA TGCTGCTCCG GGTGGTCGGC 
AGTCAGACTT CAGAATCCAT GGGCGAGGAA GACCTGCTCA GCCCTCCCCA GGACACAAGC 
ACAGGGTTAG AGGAAGTGAT GGAGCAGCTC AACCACTCCT TCCCTAGTTC CAGAGGACAC 
AATGTAGGAA GCCTTTTCCA CTTGGCAGAT GATTTGGGCA GAGCGATGGA GTCCTTAGTT 
TCAGTCATGA CAGATGAAGA AGGGGCAGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMD ( XM_014111345.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013966820.1 Canis lupus familiaris dystrophin (DMD), transcript variant X6, mRNA.

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CloneID	OCe45416
Clone ID Related Accession (Same CDS sequence)	XM_014111345.2 , XM_014111345.1
Accession Version	XM_014111345.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	dystrophin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014111345.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTGGT GGGAAGAAGT AGAGGACTGT TATGAAAGAG AAGATGTTCA AAAGAAAACA 
TTCACAAAAT GGATAAATGC ACAGTTTTCT AAGTTTGGGA AGCAGCACAT AGAGAACCTC 
TTCAGTGACC TACAGGATGG GAGACGCCTC CTAGACCTTT TGGAAGGCCT GACAGGGCAA 
AAACTGCCAA AAGAAAAAGG ATCCACAAGA GTTCATGCCC TGAACAATGT CAACAAGGCA 
CTGCGCGTCT TGCAGAAAAA TAATGTTGAT TTAGTGAACA TTGGAAGTAC TGACATAGTA 
GATGGAAATC ACAAACTGAC TCTTGGTTTG ATTTGGAATA TAATCCTCCA CTGGCAGGTC 
AAAAATGTAA TGAAAAATAT CATGGCTGGA TTGCAACAAA CCAACAGTGA AAAGATTCTC 
CTGAGCTGGG TCCGACAATC AACTCGTAAT TATCCACAGG TTAATGTCAT TAACTTCACC 
ACCAGCTGGT CTGATGGCCT GGCTTTGAAC GCTCTCATCC ACAGTCATAG GCCAGACCTG 
TTTGATTGGA ATAGTGTGGT TTGCCAGCAG TCAGCCACAC AACGCCTGGA ACATGCATTC 
AACATTGCCA AATATCAATT AGGCATAGAG AAACTGCTTG ATCCTGAAGA TGTTGCCACC 
ACTTATCCAG ATAAGAAGTC CATCTTAATG TATATCACAT CACTCTTCCA AGTTTTGCCT 
CAACAAGTGA GCATTGAAGC CATCCAGGAA GTGGAAATGT TGCCAAGGCC ATCAAAAGTT 
ACTAGAGAAG AACATTTTCA ATTACATCAT CAAATGCACT ATTCTCAACA GATCACAGTC 
AGTCTAGCAC AGGGCTATGA ACGAGCCCCT TCCTCTCCTA AGCCTCGGTT CAAGAGCTAT 
GCCTACACAC AGGCTGCTTA TGTCACCACT TCTGACCCCA CACGGAGCCC ACTTCCTTCA 
CAGCATTTGG AAACTCCTGA AGACAAGTCA TTTGGCCGGT CATTGACAGA GACCGAAGCA 
AACCTGGACA GTTATCAAAC AGCTTTGGAA GAAGTACTCT CGTGGCTTCT TTCAGCTGAG 
GATGCACTGC AAGCCCAAGG AGAGATTTCT AATGATGTCG AAGAAGTGAA AGAACAATTT 
CATACTCATG AGGGATATAT GATGGACTTG ACATCCCATC AGGGACGGGT CGGTAATGTT 
CTCCAACTGG GAAGTCAACT GATTGGAACA GGGAAATTAT CAGAAGATGA AGAAACCGAA 
GTGCAGGAAC AAATGAATCT CCTCAATTCA AGATGGGAAT GCCTCAGGGT AGCTAGCATG 
GAAAAACAAA GCAATTTACA TAAAGTTCTA ATGGATCTCC AGAATCAGCA ACTGAAAGAG 
TTAAATGACT GGCTAACCAA AACAGAAGAG AGAACAAGGA AAATGGAGAA GGAGCCCCTT 
GGACCTGATA TTGAAGACCT AAAACGCCAA GTACAACAAC ATAAGGTGCT TCAAGAAGAC 
TTAGAACAGG AACAAGTCAG GGTCAATTCC CTCACTCATA TGGTGGTGGT AGTCGATGAA 
TCTAGTGGAG ACCATGCAAC TGCTGCTTTG GAAGAACAAC TTAAGGTACT GGGAGATCGA 
TGGGCAAACA TCTGTAGGTG GACAGAAGAT CGCTGGGTTC TTTTACAAGA CATCCTCCTA 
AAATGGCAGC GTTTTACTGA AGAACAGTGC CTTTTCAGTG CATGGCTTTC GGAGAAGGAA 
GATGCAGTGA ACAAGATTCA CACAACTGGC TTTAAGGATC AAAGTGAAGT GTTATCAAAT 
CTTCAGAAAC TGGCTGTCTT AAAAACAGAT CTGGAAAAGA AGAAGCAAAC CATGGACAAA 
CTCTGCTCAC TCAACCAAGA CCTTCTTTCA GCGCTGAAAA ACACAGTGGT AGCCCACAAG 
ATGGAAGCAT GGCTGGACAA CTTTGCCCAG CGCTGGGATA ATTTAGTCCA GAAACTTGAA 
AAAAGTTCAG CACAGATTTC ACAGGCTGTC ACCACCACTC AGCCATCACT AACACAGACA 
ACTGTAATGG AAACAGTAAC TATGGTGACC ACGAGGGAAC ACATCTTGGT AAAGCATGCC 
CAAGAGGAAC TGCCACCACC ACCCCCTCAG AAGAAGAGGC AGATTATCGT GGATTCTGAA 
ATTAGGAAAA GGTTGGATGT CGATATAACT GAACTTCACA GTTGGATTAC TCGTTCAGAA 
GCTGTGTTGC AGAGTCCTGA ATTTGCAATC TATCGGAAGG AAGGCAACTT CTCAGACCTT 
AAAGAAAAAG TCAATGCCAT AGAGCGAGAA AAAGCCGAGA AGTTCAGAAA ACTGCAAGAT 
GCCAGCAGAT CAGCTCAGGC CCTGGTGGAA CAGATGGTGA ATGAGGGTGT TAATGCTGAC 
AGCATCAAAC AAGCCTCCGA ACAACTGAAC AGCCGGTGGA TAGAGTTCTG CCAATTGCTA 
AGCGAGAGAC TTAACTGGCT GGAGTATCAG AACAACATCA TCACTTTCTA TAATCAGCTA 
CAACAATTGG AGCAGATGAC AACTACTGCT GAAAACTGGT TGAAAACCCA GCCTACCACC 
ACATCAGAGC CAACAGCAAT TAAAAGCCAG TTAAAAATTT GTAAGGATGA AATCAACCGA 
CTGTCAGCTC TTCAGCCTCA AATCGAGCGA TTAAAAATTC AAAGCATAGC CCTGAAAGAG 
AAAGGACAAG GGCCAATGTT CCTGGATGCA GACTTTGTGG CCTTTACAAA TCATTTTAAC 
CAAGTCTTTG CTGATGTGCA GGCAAGAGAA AAAGAGCTAC AAACAATTTT TGACAGTTTG 
CCACCCATGC GCTATCAGGA GACTATGAGT ACCATCCTGA CATGGATCCA GCAGTCAGAA 
ACCAAACTCT CTATACCTCA GGTTACTGTC ACTGAATATG ACATCATGGA ACAGAGACTC 
GGAGAGCTAC AGGCTTTACA AAGCTCTCTG CAAGAGCAAC AAAATGGCCT AAACTATCTC 
AGCACCACTG TGAAAGAGAT GTCAAAGAAA GCACCACTGT CTGATATTAG TCGGAAATAT 
CAATCAGAAT TTGAAGAGAT TGAGGGACGT TGGAAGAAGC TGTCTTCCCA GCTGGTTGAA 
CATTGTCAAA AGTTGGAGGA GCAAATGGCT AAACTTCGAA AAATTCAGAA TCACATAAAA 
ACTCTGAAGA AATGGATCAC TGAAGTCGAT GTTTTCCTGA AGGAGGAATG GCCTGCCCTT 
GGGGATTCAG AAATTCTGAA AAGACAGCTG AAACAGTGCA GGCTTTTAGT CAATGACATT 
CAGACCATCC AGCCTAGTCT CAACAGTGTC AATGAAGGGG CTCAGAAGAT GAAGAATGAA 
GCAGAACCAG AGTTTGCTGG CAGACTTGAG ACAGAGCTCC GAGAACTTAA CACCCAGTGG 
GATTACATGT GCCGCCAGGT CTATGCCAGG AAGGAAGCCT TAAAAGGAGG TTTGGATAAA 
ACTGTAAGTC TTCAGAAAGA TCTGTCAGAG ATGCATGAGT GGATGACACA AGCTGAAGAA 
GAATACCTAG AGAGAGATTT CGAATACAAG ACCCCTGATG AATTACAGAC AGCAGTTGAA 
GAGATGAAGA GAGCTAAAGA AGAGGCCCAG CAAAAAGAAG CAAAAGTGAA ACTCCTAACC 
GAGTCCGTCA ATAGTGTCAT AGCTCAGGCT CCACCTGCAG CACAAGAGGC CTTAAAAAAG 
GAACTTGACA CTCTCACCAC CAACTACCAG TGGCTCTGCA CCAGGCTCAA TGGCAAATGC 
AAGACCTTGG AAGAAGTTTG GGCGTGCTGG CATGAGTTAT TGTCCTACTT GGAGAAGGCA 
AACAAGTGGC TAAGTGAAGT AGAAGTCAAG CTTAAAACCA CTGAAAATAT TTCTGGGGGA 
GCTGAGGAAA TCGCCGAGGT GCTTGATTCG CTTGAAAATT TGATGCAACA TTCAGAGGAT 
AACCCGAATC AGATTCGCAT ATTGGCACAG ACCTTGACAG ATGGTGGAGT CATGGATGAA 
CTGATCAATG AGGAGCTTGA GACATTTAAT TCTCGTTGGA GAGAACTCCA TGAAGAGGCT 
GTGAGGAGGC AAAAGTTGCT TGAGCAGAGT ATCCAGTCGG CCCAGGAGAT AGAAAAATCC 
TTGCACTTAA TTCAGGAGTC CCTCTCTTCC ATTGACAAGC AGTTGGCAGC TTATATTGCT 
GACAAAGTGG ATGCAGCTCA GATGCCTCAG GAAGCCCAGA AAATCCAATC AGATTTGACA 
AGTCATGAGA TCAGTTTAGA AGAAATGAAG AAACATAACC AGGGAAAGGA GACTGCCCAA 
AGGGTACTAT CCCAAATTGA TGTGGCACAG AAAAAATTGC AGGATGTTTC CATGAAGTTT 
CGATTATTCC AGAAGCCAGC CAATTTTGAG CAGCGCCTAC AAGAAAGTAA AATGATTTTA 
GATGAAGTGA AGATGCATTT ACCTGCGTTG GAAACAAAGA GTGTGGAACA GGAAGTAGTA 
CAGTCACAGT TAAATCATTG TGTGAACTTG TATAAAAGTC TGAGTGAAGT GAAGTCTGAA 
GTGGAAATGG TAATAAAAAC TGGACGTCAG ATTGTACAGA AGAAGCAGAC GGAAAACCCG 
AAAGAGCTTG ATGAAAGAGT TACAGCTTTG AAATTGCATT ATAATGAGCT GGGAGCAAAG 
GTGACAGAAA GAAAGCAACA GTTGGAAAAA TGCTTGAAAT TGTCCCGTAA GATGCGAAAG 
GAAATGAATG CCCTGACAGA ATGGCTGGCA GCGACAGATA TGGAACTGAC GAAGAGATCG 
GCAGTTGAAG GAATGCCTAG TAATTTGGAT TCTGAAGTTG CCTGGGGAAA GGCTACTCAG 
AAAGAGATTG AGAAACAGAA GGTTCACCTA AAGAGTGTCA CAGAGGTAGG AGAGGCCTTG 
AAAACGGTTT TGGGCAAGAA GGAAATGTTG GTGGAAGATA AACTGAGTCT TCTGAATAGT 
AACTGGATAG CCGTCACTTC CCGAGCAGAA GAGTGGTTAA ACCTTTTATT GGAATACCAG 
AAACACATGG AAAACTTTGA CCAGAATGTG GATTACATCA CAAACTGGAT CATTCAGGCT 
GATGCACTTT TGGATGAATC TGAGAAAAAG AAACCTCAGC AAAAAGAAGA CATACTTAAG 
CGTTTAAAGG CTGAAATGAA TGACATACGT CCAAAGGTGG ATTCTACACG TGACCAAGCA 
GCAAACCTGA TGGCAAACCG CGGCGACCAC TGCAGGAAAG TAGTAGAGCC CAAAATCTCA 
GAGCTCAACC ATCGATTTGC AGCCATTTCT CACAGAATTA AGACTGGAAA GGCCTCCATT 
CCTTTGAAGG AATTGGAGCA GTTTAACTCA GATATACAAA AATTGCTTGA ACCACTGGAG 
GCTGAAATTC AGCAGGGGGT GAATCTGAAA GAGGAAGACT TCAATAAAGA TATGAGTGAA 
GACAATGAGG GTACTGTAAA AGAATTGTTG CAAAGAGGAG ACAACTTACA ACAAAGAATC 
ACAGATGAGA GAAAGCGAGA GGAAATAAAG ATAAAACAAC AGCTGTTACA GACAAAACAT 
AATGCTCTCA AGGATTTGAG GTCTCAAAGA AGAAAAAAGG CTCTAGAAAT TTCTCACCAG 
TGGTATCAGT ACAAGAGGCA GGCTGATGAT CTCCTGAAAT GCTTGGATGA CATTGAAAAA 
AAATTAGCCA GCCTACCTGA ACCCAGAGAT GAAAGGAAAA TAAAGGAAAT TGATCGTGAA 
TTGCAGAAGA AGAAAGAGGA GCTGAATGCA GTGCGTAGGC AAGCTGAGGG CTTGTCTGAG 
GATGGGGCCG CAATGGCAGT GGAGCCAACT CAGATCCAGC TCAGCAAGCG CTGGCGGGAA 
ATTGAGAGCA AATTTGCTCA GTTTCGAAGA CTCAACTTTG CACAAATTCA CACTGTCCAT 
GAAGAGTCAG TGGTGGCGAT GACTGAAGAC ATGCCTTTGG AAATTTCTTA TGTGCCTTCT 
ACTTACCTGA CTGAGATCAC TCATGTCTCA CAAGCCCTAT CAGAAGTGGA AGAGCTTCTT 
AATGCTCCCG ACCTTTGTGC TCAAGATTTT GAAGATCTCT TTAAACAAGA GGAATCCTTG 
AAGAACATAA AAGACAGCCT GCAACAAATC TCAGGTCGGA TTGACATCAT TCACAATAAA 
AAGACAGCAG CATTGCACAG TGCCACTCCT GCAGAAAGGG CAAAGCTCCA GGAAGCTCTC 
TCACGGCTTG ATTTCCAATG GGAAAGAGTT AACAATATGT ACAAGGACCG ACAAGGGAGA 
TTTGACAGAT CTGTGGAAAA ATGGCGGCGG TTTCATTATG ATATGAAGAT ACTTAATCAA 
TGGCTAACAG AAGCTGAACA GTTTCTCAAA AAGACACAAA TTCCTGAGAA TTGGGAACAT 
GCCAAATACA AATGGTATCT TAAGGAACTC CAGGATGGCA TTGGACAGCG GCAAAGTGTT 
GTCAGGGTAT TGAATGCAAC TGGGGAAGAA ATAATTCAAC AGTCCTCAAA AACAGATGCC 
AGTATTCTCC AAGAAAAACT GGGAAGCCTG AATCTGCGGT GGCAGGAGGT CTGCAAACAG 
CTGGCAGAAA GAAAAAAGAG GCTAGAGGAA CAGAAGAATA TCTTGTCAGA ATTTCAAAGA 
GATGTAAATG AATTTGTTTT ATGGTTGGAA GAAGCGGATA ACGTTGCTAA TATTCCACTT 
GAACCTGGAA ATGAGCAGCA GCTAAAAGAA AAACTTGAAC AAGTCAAGTT ACTGGCAGAA 
GAGTTGCCCC TGCGCCAGGG AATTCTAAAA CAATTAAATG AAACTGGAGG AACAGTGCTT 
GTAAGTGCTC CCCTAAGCCC AGAAGAGCAA GATAAACTTG AAAATAAGCT CAAGCAGACA 
AATCTTCAGT GGATAAAGGT TTCTAGAAAT CTGCCTGAGA AGCAAGAAGA AATTGAGGCA 
CACGTAAAAG ACCTTGGACA GCTGGAAGAG CAGTTAAATC ATCTGCTTCT ATGGCTGTCT 
CCTATTAGGA ATCAGTTGGA AATTTACAAT CAGCCAAATC AAACAGGACC ATTTGACATC 
AAGGAAATTG AAGTAGCAGT TCAAGCTAAA CAGCCGGATG TGGAAGGGAT TTTGTCTAAA 
GGGCAGCATT TGTACAAGGA AAAACCAGCC ACTCAGCCAG CGAAGAGAAA GCTGGAAGAT 
CTCAGCTCTG ATTGGAAGGT GGTAACTCAG TTGCTTCAAG AGCTGCGGGC AAAGCAGCCT 
GGCCCAGCTC CTGGACTGAC CACTGTCAGA GCCCCTCCCA GTCAGACTGT TACTCTGGTG 
ACACAACCCG CGGTTACCAA GGAAACTGCC ATCTCCAAAC TAGAAATGCC ATCTTCATTG 
CTGTTGGAGG TACCTGCACT GGCAGATTTC AACCGAGCTT GGACAGAACT TACCGACTGG 
CTGTCTCTGC TTGATCGAGT TATAAAATCA CAGAGGGTGA TGGTGGGTGA TCTTGAAGAC 
ATTAACGAGA TGATCATCAA GCAGAAGGCA ACGCTGCAGG ATTTGGAACA GAGGCGCCCC 
CAGTTGGAAG AACTCATTAC CGCTGCCCAG AATTTGAAAA ACAAGACCAG CAATCAAGAG 
GCTAGAACAA TCATTACTGA TCGAATTGAA AGAATTCAGA GTCAGTGGGA TGAAGTACAG 
GAACATCTTC AGAACCGGAG GCAACAGTTG AATGAAATGT TAAAGGATTC CACACAATGG 
CTGGAAGCTA AAGAGGAGGC TGAGCAGGTG TTGGGGCAGG CCAGAGCCAA GCTTGAGTCA 
TGGAAGGAGG CTCCCTACAC AGTAGATGCA ATCCAAAAGA AAATCACAGA AACCAAGTTG 
GCCAAAGACC TCCGCCAGTG GCAGATAAAT GTAGATGTTG CAAATGATTT GGCACTGAAA 
CTTCTCCGAG ATTATTCTGC AGATGATACC AGAAAAGTAC ACATGATAAC AGAGAACATC 
AATGCCTCTT GGGCAAGCAT CCATAAAAGA TTGAGTGAGC GAGAGGCTGC TCTGGAAGAA 
ACCCACAGAT TACTGCAACA GTTCCCCTTG GACCTGGAGA AGTTCCTTGC CTGGCTTACA 
GAAGCCGAAA CAACTGCCAA CGTCCTGCAG GATGCCACCC ATAAGGAAAG GCTTCTAGAA 
GATTCCAAGG GAGTAAGAGA GCTGATGAAA CAATGGCAAG ATCTCCAAGG AGAAATCGAA 
GCTCACACAG ATATCTATCA CAACCTGGAC GAAAATGGCC AAAAAGTCCT GAGATCCCTG 
GAAGGTTCTG ACGATGCAGC CTTGTTGCAA AGACGTTTGG ATAACATGAA CTTCAAGTGG 
AGCGAACTTC GGAAAAAGTC TCTCAACATT AGGTCTCACT TGGAAGCCAG TTCTGACCAG 
TGGAAGCGTC TGCACCTTTC TCTTCAGGAA CTTCTGGTAT GGCTCCAGCT GAAAGATGAT 
GAGTTAAGCC GGCAGGCACC CATTGGAGGA GACTTTCCAG CGGTGCAGAA GCAGAATGAT 
GTACACAGGG CCTTCAAGAG GGAATTGAAA ACGAAAGAAC CTGTAATCAT GAGTACTCTT 
GAGACTGTAC GAATATTTCT GACAGAGCAG CCTTTAGAAG GACTAGAGAA ACTCTACCAG 
GAGCCCAGAG AGCTGCCTCC TGAAGAGAGA GCCCAGAATG TCACACGGCT CCTACGAAAG 
CAAGCTGAGG AGGTCAACAC TCAGTGGGAA AAACTGAACG TGCACTCTGC AGACTGGCAG 
AGAAAAATAG ACGAGGCCCT CGAAAGACTC CAGGAGCTTC AGGAAGCAAC AGATGAGCTG 
GATCTCAAAC TACGTCAGGC AGAGGTGATC AAAGGATCCT GGCAGCCTGT GGGTGATCTC 
CTCATTGACT CTCTCCAAGA TCACCTCGAA AAAGTCAAGG CGCTTCGAGG AGAAATTACA 
CCTCTGAAAG AGAATGTCAG CTACGTCAAT GACCTTGCTC GCCAACTCAC TACGTTGGGC 
ATTCAGCTGT CACCATATAA CCTCAACACT CTGGAAGACC TGAACACCAG ATGGAAGCTT 
CTGCAGGTGG CCATTGAGGA CCGCATCAGG CAGCTGCATG AAGCGCACAG GGACTTTGGA 
CCAGCCTCCC AGCACTTCCT TTCCACTTCT GTCCAGGGTC CCTGGGAGAG AGCCATCTCA 
CCAAACAAAG TGCCCTACTA TATCAACCAC GAGACCCAAA CAACTTGCTG GGACCATCCC 
AAAATGACAG AGCTCTACCA GTCTTTAGCT GACCTGAATA ATGTCAGGTT CTCAGCTTAC 
AGGACTGCCA TGAAACTCCG AAGACTGCAG AAGGCCCTTT GCTTGGATCT CTTGAGCCTA 
TCGGCTGCAT GCGATGCCTT GGACCAGCAC AACCTCAAGC AAAATGACCA GCCCATGGAT 
ATCCTGCAGG TCATTAACTG TCTGACCACT ATTTATGATC GCCTAGAGCA AGAGCACAAC 
AATCTGGTCA ACGTCCCTCT CTGCGTGGAT ATGTGTCTCA ATTGGCTGCT GAATGTTTAT 
GACACGGGAC GAACGGGGAG GATCCGGGTC CTGTCTTTTA AAACTGGCAT CATTTCTCTG 
TGTAAAGCCC ATTTGGAAGA CAAGTACAGA TACCTCTTCA AGCAAGTGGC AAGTTCGACA 
GGATTTTGTG ACCAGCGCAG GCTGGGCCTC CTCCTGCATG ACTCTATCCA GATCCCAAGA 
CAGTTGGGTG AAGTCGCATC GTTCGGGGGC AGTAACATTG AGCCGAGTGT CAGGAGCTGC 
TTCCAGTTTG CTAATAATAA GCCTGAGATC GAAGCGGCCC TCTTCCTAGA CTGGATGCGC 
CTGGAGCCCC AGTCCATGGT GTGGCTGCCT GTCCTGCACC GAGTGGCTGC CGCGGAAACT 
GCCAAGCACC AGGCCAAGTG CAACATCTGC AAGGAGTGTC CCATCATCGG ATTCAGGTAC 
AGGAGTCTAA AGCACTTTAA TTATGACATC TGCCAAAGTT GCTTTTTTTC TGGTCGAGTT 
GCAAAAGGCC ATAAAATGCA CTATCCCATG GTGGAATACT GCACTCCGAC TACATCGGGA 
GAAGATGTCC GTGACTTTGC CAAGGTACTA AAAAACAAAT TTCGAACCAA AAGGTATTTT 
GCGAAGCATC CCCGAATGGG CTACCTGCCA GTGCAGACTG TCTTAGAGGG GGACAACATG 
GAAACTCCTG TCACTCTGAT CAACTTCTGG CCGGTAGATT CTGCGCCTGC CTCGTCCCCT 
CAGCTTTCAC ACGATGATAC TCATTCACGC ATTGAGCATT ATGCTAGCAG GCTAGCAGAA 
ATGGAAAACA GCAATGGATC TTATCTAAAT GATAGCATCT CTCCTAATGA GAGCATAGAT 
GATGAACATT TGTTAATCCA GCATTACTGC CAAAGTTTGA ACCAGGACTC CCCCCTGAGC 
CAGCCTCGTA GTCCTGCCCA GATCTTGATT TCCTTAGAGA GTGAGGAAAG AGGGGAGCTA 
GAGAGAATCC TAGCAGATCT TGAGGAAGAA AACAGAAATC TGCAAGCAGA GTATGATCGT 
CTAAAGCAGC AGCATGAACA CAAAGGCCTG TCCCCACTGC CATCCCCTCC TGAAATGATG 
CCTACTTCTC CCCAAAGTCC CCGGGATGCT GAGCTCATCG CTGAGGCCAA GCTGCTGCGT 
CAACACAAAG GCCGCCTGGA AGCCAGGATG CAAATCCTTG AAGACCATAA CAAACAACTG 
GAATCCCAGT TACACAGGCT CAGGCAGCTG CTGGAGCAAC CCCAGGCAGA GGCCAAGGTG 
AATGGTACAA CGGTGTCTTC TCCTTCTACC TCTCTTCAGA GGTCAGATAG CAGTCAGCCT 
ATGCTGCTCC GGGTGGTCGG CAGTCAGACT TCAGAATCCA TGGGCGAGGA AGACCTGCTC 
AGCCCTCCCC AGGACACAAG CACAGGGTTA GAGGAAGTGA TGGAGCAGCT CAACCACTCC 
TTCCCTAGTT CCAGAGGAAG AAATACCCCT GGGAAGCCAA TGAGAGAGGA CACAATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013966820.1
CDS	247..11286
Translation	MLWWEEVEDCYEREDVQKKTFTKWINAQFSKFGKQHIENLFSDLQDGRRLLDLLEGLTGQKLPKEKGSTRVHALNNVNKALRVLQKNNVDLVNIGSTDIVDGNHKLTLGLIWNIILHWQVKNVMKNIMAGLQQTNSEKILLSWVRQSTRNYPQVNVINFTTSWSDGLALNALIHSHRPDLFDWNSVVCQQSATQRLEHAFNIAKYQLGIEKLLDPEDVATTYPDKKSILMYITSLFQVLPQQVSIEAIQEVEMLPRPSKVTREEHFQLHHQMHYSQQITVSLAQGYERAPSSPKPRFKSYAYTQAAYVTTSDPTRSPLPSQHLETPEDKSFGRSLTETEANLDSYQTALEEVLSWLLSAEDALQAQGEISNDVEEVKEQFHTHEGYMMDLTSHQGRVGNVLQLGSQLIGTGKLSEDEETEVQEQMNLLNSRWECLRVASMEKQSNLHKVLMDLQNQQLKELNDWLTKTEERTRKMEKEPLGPDIEDLKRQVQQHKVLQEDLEQEQVRVNSLTHMVVVVDESSGDHATAALEEQLKVLGDRWANICRWTEDRWVLLQDILLKWQRFTEEQCLFSAWLSEKEDAVNKIHTTGFKDQSEVLSNLQKLAVLKTDLEKKKQTMDKLCSLNQDLLSALKNTVVAHKMEAWLDNFAQRWDNLVQKLEKSSAQISQAVTTTQPSLTQTTVMETVTMVTTREHILVKHAQEELPPPPPQKKRQIIVDSEIRKRLDVDITELHSWITRSEAVLQSPEFAIYRKEGNFSDLKEKVNAIEREKAEKFRKLQDASRSAQALVEQMVNEGVNADSIKQASEQLNSRWIEFCQLLSERLNWLEYQNNIITFYNQLQQLEQMTTTAENWLKTQPTTTSEPTAIKSQLKICKDEINRLSALQPQIERLKIQSIALKEKGQGPMFLDADFVAFTNHFNQVFADVQAREKELQTIFDSLPPMRYQETMSTILTWIQQSETKLSIPQVTVTEYDIMEQRLGELQALQSSLQEQQNGLNYLSTTVKEMSKKAPLSDISRKYQSEFEEIEGRWKKLSSQLVEHCQKLEEQMAKLRKIQNHIKTLKKWITEVDVFLKEEWPALGDSEILKRQLKQCRLLVNDIQTIQPSLNSVNEGAQKMKNEAEPEFAGRLETELRELNTQWDYMCRQVYARKEALKGGLDKTVSLQKDLSEMHEWMTQAEEEYLERDFEYKTPDELQTAVEEMKRAKEEAQQKEAKVKLLTESVNSVIAQAPPAAQEALKKELDTLTTNYQWLCTRLNGKCKTLEEVWACWHELLSYLEKANKWLSEVEVKLKTTENISGGAEEIAEVLDSLENLMQHSEDNPNQIRILAQTLTDGGVMDELINEELETFNSRWRELHEEAVRRQKLLEQSIQSAQEIEKSLHLIQESLSSIDKQLAAYIADKVDAAQMPQEAQKIQSDLTSHEISLEEMKKHNQGKETAQRVLSQIDVAQKKLQDVSMKFRLFQKPANFEQRLQESKMILDEVKMHLPALETKSVEQEVVQSQLNHCVNLYKSLSEVKSEVEMVIKTGRQIVQKKQTENPKELDERVTALKLHYNELGAKVTERKQQLEKCLKLSRKMRKEMNALTEWLAATDMELTKRSAVEGMPSNLDSEVAWGKATQKEIEKQKVHLKSVTEVGEALKTVLGKKEMLVEDKLSLLNSNWIAVTSRAEEWLNLLLEYQKHMENFDQNVDYITNWIIQADALLDESEKKKPQQKEDILKRLKAEMNDIRPKVDSTRDQAANLMANRGDHCRKVVEPKISELNHRFAAISHRIKTGKASIPLKELEQFNSDIQKLLEPLEAEIQQGVNLKEEDFNKDMSEDNEGTVKELLQRGDNLQQRITDERKREEIKIKQQLLQTKHNALKDLRSQRRKKALEISHQWYQYKRQADDLLKCLDDIEKKLASLPEPRDERKIKEIDRELQKKKEELNAVRRQAEGLSEDGAAMAVEPTQIQLSKRWREIESKFAQFRRLNFAQIHTVHEESVVAMTEDMPLEISYVPSTYLTEITHVSQALSEVEELLNAPDLCAQDFEDLFKQEESLKNIKDSLQQISGRIDIIHNKKTAALHSATPAERAKLQEALSRLDFQWERVNNMYKDRQGRFDRSVEKWRRFHYDMKILNQWLTEAEQFLKKTQIPENWEHAKYKWYLKELQDGIGQRQSVVRVLNATGEEIIQQSSKTDASILQEKLGSLNLRWQEVCKQLAERKKRLEEQKNILSEFQRDVNEFVLWLEEADNVANIPLEPGNEQQLKEKLEQVKLLAEELPLRQGILKQLNETGGTVLVSAPLSPEEQDKLENKLKQTNLQWIKVSRNLPEKQEEIEAHVKDLGQLEEQLNHLLLWLSPIRNQLEIYNQPNQTGPFDIKEIEVAVQAKQPDVEGILSKGQHLYKEKPATQPAKRKLEDLSSDWKVVTQLLQELRAKQPGPAPGLTTVRAPPSQTVTLVTQPAVTKETAISKLEMPSSLLLEVPALADFNRAWTELTDWLSLLDRVIKSQRVMVGDLEDINEMIIKQKATLQDLEQRRPQLEELITAAQNLKNKTSNQEARTIITDRIERIQSQWDEVQEHLQNRRQQLNEMLKDSTQWLEAKEEAEQVLGQARAKLESWKEAPYTVDAIQKKITETKLAKDLRQWQINVDVANDLALKLLRDYSADDTRKVHMITENINASWASIHKRLSEREAALEETHRLLQQFPLDLEKFLAWLTEAETTANVLQDATHKERLLEDSKGVRELMKQWQDLQGEIEAHTDIYHNLDENGQKVLRSLEGSDDAALLQRRLDNMNFKWSELRKKSLNIRSHLEASSDQWKRLHLSLQELLVWLQLKDDELSRQAPIGGDFPAVQKQNDVHRAFKRELKTKEPVIMSTLETVRIFLTEQPLEGLEKLYQEPRELPPEERAQNVTRLLRKQAEEVNTQWEKLNVHSADWQRKIDEALERLQELQEATDELDLKLRQAEVIKGSWQPVGDLLIDSLQDHLEKVKALRGEITPLKENVSYVNDLARQLTTLGIQLSPYNLNTLEDLNTRWKLLQVAIEDRIRQLHEAHRDFGPASQHFLSTSVQGPWERAISPNKVPYYINHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQVINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPVTLINFWPVDSAPASSPQLSHDDTHSRIEHYASRLAEMENSNGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKQQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDTSTGLEEVMEQLNHSFPSSRGRNTPGKPMREDTM

Target ORF information:

RefSeq Version	XM_014111345.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris dystrophin (DMD), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111345.2

ORF Insert Sequence:

ATGCTTTGGT GGGAAGAAGT AGAGGACTGT TATGAAAGAG AAGATGTTCA AAAGAAAACA 
TTCACAAAAT GGATAAATGC ACAGTTTTCT AAGTTTGGGA AGCAGCACAT AGAGAACCTC 
TTCAGTGACC TACAGGATGG GAGACGCCTC CTAGACCTTT TGGAAGGCCT GACAGGGCAA 
AAACTGCCAA AAGAAAAAGG ATCCACAAGA GTTCATGCCC TGAACAATGT CAACAAGGCA 
CTGCGCGTCT TGCAGAAAAA TAATGTTGAT TTAGTGAACA TTGGAAGTAC TGACATAGTA 
GATGGAAATC ACAAACTGAC TCTTGGTTTG ATTTGGAATA TAATCCTCCA CTGGCAGGTC 
AAAAATGTAA TGAAAAATAT CATGGCTGGA TTGCAACAAA CCAACAGTGA AAAGATTCTC 
CTGAGCTGGG TCCGACAATC AACTCGTAAT TATCCACAGG TTAATGTCAT TAACTTCACC 
ACCAGCTGGT CTGATGGCCT GGCTTTGAAC GCTCTCATCC ACAGTCATAG GCCAGACCTG 
TTTGATTGGA ATAGTGTGGT TTGCCAGCAG TCAGCCACAC AACGCCTGGA ACATGCATTC 
AACATTGCCA AATATCAATT AGGCATAGAG AAACTGCTTG ATCCTGAAGA TGTTGCCACC 
ACTTATCCAG ATAAGAAGTC CATCTTAATG TATATCACAT CACTCTTCCA AGTTTTGCCT 
CAACAAGTGA GCATTGAAGC CATCCAGGAA GTGGAAATGT TGCCAAGGCC ATCAAAAGTT 
ACTAGAGAAG AACATTTTCA ATTACATCAT CAAATGCACT ATTCTCAACA GATCACAGTC 
AGTCTAGCAC AGGGCTATGA ACGAGCCCCT TCCTCTCCTA AGCCTCGGTT CAAGAGCTAT 
GCCTACACAC AGGCTGCTTA TGTCACCACT TCTGACCCCA CACGGAGCCC ACTTCCTTCA 
CAGCATTTGG AAACTCCTGA AGACAAGTCA TTTGGCCGGT CATTGACAGA GACCGAAGCA 
AACCTGGACA GTTATCAAAC AGCTTTGGAA GAAGTACTCT CGTGGCTTCT TTCAGCTGAG 
GATGCACTGC AAGCCCAAGG AGAGATTTCT AATGATGTCG AAGAAGTGAA AGAACAATTT 
CATACTCATG AGGGATATAT GATGGACTTG ACATCCCATC AGGGACGGGT CGGTAATGTT 
CTCCAACTGG GAAGTCAACT GATTGGAACA GGGAAATTAT CAGAAGATGA AGAAACCGAA 
GTGCAGGAAC AAATGAATCT CCTCAATTCA AGATGGGAAT GCCTCAGGGT AGCTAGCATG 
GAAAAACAAA GCAATTTACA TAAAGTTCTA ATGGATCTCC AGAATCAGCA ACTGAAAGAG 
TTAAATGACT GGCTAACCAA AACAGAAGAG AGAACAAGGA AAATGGAGAA GGAGCCCCTT 
GGACCTGATA TTGAAGACCT AAAACGCCAA GTACAACAAC ATAAGGTGCT TCAAGAAGAC 
TTAGAACAGG AACAAGTCAG GGTCAATTCC CTCACTCATA TGGTGGTGGT AGTCGATGAA 
TCTAGTGGAG ACCATGCAAC TGCTGCTTTG GAAGAACAAC TTAAGGTACT GGGAGATCGA 
TGGGCAAACA TCTGTAGGTG GACAGAAGAT CGCTGGGTTC TTTTACAAGA CATCCTCCTA 
AAATGGCAGC GTTTTACTGA AGAACAGTGC CTTTTCAGTG CATGGCTTTC GGAGAAGGAA 
GATGCAGTGA ACAAGATTCA CACAACTGGC TTTAAGGATC AAAGTGAAGT GTTATCAAAT 
CTTCAGAAAC TGGCTGTCTT AAAAACAGAT CTGGAAAAGA AGAAGCAAAC CATGGACAAA 
CTCTGCTCAC TCAACCAAGA CCTTCTTTCA GCGCTGAAAA ACACAGTGGT AGCCCACAAG 
ATGGAAGCAT GGCTGGACAA CTTTGCCCAG CGCTGGGATA ATTTAGTCCA GAAACTTGAA 
AAAAGTTCAG CACAGATTTC ACAGGCTGTC ACCACCACTC AGCCATCACT AACACAGACA 
ACTGTAATGG AAACAGTAAC TATGGTGACC ACGAGGGAAC ACATCTTGGT AAAGCATGCC 
CAAGAGGAAC TGCCACCACC ACCCCCTCAG AAGAAGAGGC AGATTATCGT GGATTCTGAA 
ATTAGGAAAA GGTTGGATGT CGATATAACT GAACTTCACA GTTGGATTAC TCGTTCAGAA 
GCTGTGTTGC AGAGTCCTGA ATTTGCAATC TATCGGAAGG AAGGCAACTT CTCAGACCTT 
AAAGAAAAAG TCAATGCCAT AGAGCGAGAA AAAGCCGAGA AGTTCAGAAA ACTGCAAGAT 
GCCAGCAGAT CAGCTCAGGC CCTGGTGGAA CAGATGGTGA ATGAGGGTGT TAATGCTGAC 
AGCATCAAAC AAGCCTCCGA ACAACTGAAC AGCCGGTGGA TAGAGTTCTG CCAATTGCTA 
AGCGAGAGAC TTAACTGGCT GGAGTATCAG AACAACATCA TCACTTTCTA TAATCAGCTA 
CAACAATTGG AGCAGATGAC AACTACTGCT GAAAACTGGT TGAAAACCCA GCCTACCACC 
ACATCAGAGC CAACAGCAAT TAAAAGCCAG TTAAAAATTT GTAAGGATGA AATCAACCGA 
CTGTCAGCTC TTCAGCCTCA AATCGAGCGA TTAAAAATTC AAAGCATAGC CCTGAAAGAG 
AAAGGACAAG GGCCAATGTT CCTGGATGCA GACTTTGTGG CCTTTACAAA TCATTTTAAC 
CAAGTCTTTG CTGATGTGCA GGCAAGAGAA AAAGAGCTAC AAACAATTTT TGACAGTTTG 
CCACCCATGC GCTATCAGGA GACTATGAGT ACCATCCTGA CATGGATCCA GCAGTCAGAA 
ACCAAACTCT CTATACCTCA GGTTACTGTC ACTGAATATG ACATCATGGA ACAGAGACTC 
GGAGAGCTAC AGGCTTTACA AAGCTCTCTG CAAGAGCAAC AAAATGGCCT AAACTATCTC 
AGCACCACTG TGAAAGAGAT GTCAAAGAAA GCACCACTGT CTGATATTAG TCGGAAATAT 
CAATCAGAAT TTGAAGAGAT TGAGGGACGT TGGAAGAAGC TGTCTTCCCA GCTGGTTGAA 
CATTGTCAAA AGTTGGAGGA GCAAATGGCT AAACTTCGAA AAATTCAGAA TCACATAAAA 
ACTCTGAAGA AATGGATCAC TGAAGTCGAT GTTTTCCTGA AGGAGGAATG GCCTGCCCTT 
GGGGATTCAG AAATTCTGAA AAGACAGCTG AAACAGTGCA GGCTTTTAGT CAATGACATT 
CAGACCATCC AGCCTAGTCT CAACAGTGTC AATGAAGGGG CTCAGAAGAT GAAGAATGAA 
GCAGAACCAG AGTTTGCTGG CAGACTTGAG ACAGAGCTCC GAGAACTTAA CACCCAGTGG 
GATTACATGT GCCGCCAGGT CTATGCCAGG AAGGAAGCCT TAAAAGGAGG TTTGGATAAA 
ACTGTAAGTC TTCAGAAAGA TCTGTCAGAG ATGCATGAGT GGATGACACA AGCTGAAGAA 
GAATACCTAG AGAGAGATTT CGAATACAAG ACCCCTGATG AATTACAGAC AGCAGTTGAA 
GAGATGAAGA GAGCTAAAGA AGAGGCCCAG CAAAAAGAAG CAAAAGTGAA ACTCCTAACC 
GAGTCCGTCA ATAGTGTCAT AGCTCAGGCT CCACCTGCAG CACAAGAGGC CTTAAAAAAG 
GAACTTGACA CTCTCACCAC CAACTACCAG TGGCTCTGCA CCAGGCTCAA TGGCAAATGC 
AAGACCTTGG AAGAAGTTTG GGCGTGCTGG CATGAGTTAT TGTCCTACTT GGAGAAGGCA 
AACAAGTGGC TAAGTGAAGT AGAAGTCAAG CTTAAAACCA CTGAAAATAT TTCTGGGGGA 
GCTGAGGAAA TCGCCGAGGT GCTTGATTCG CTTGAAAATT TGATGCAACA TTCAGAGGAT 
AACCCGAATC AGATTCGCAT ATTGGCACAG ACCTTGACAG ATGGTGGAGT CATGGATGAA 
CTGATCAATG AGGAGCTTGA GACATTTAAT TCTCGTTGGA GAGAACTCCA TGAAGAGGCT 
GTGAGGAGGC AAAAGTTGCT TGAGCAGAGT ATCCAGTCGG CCCAGGAGAT AGAAAAATCC 
TTGCACTTAA TTCAGGAGTC CCTCTCTTCC ATTGACAAGC AGTTGGCAGC TTATATTGCT 
GACAAAGTGG ATGCAGCTCA GATGCCTCAG GAAGCCCAGA AAATCCAATC AGATTTGACA 
AGTCATGAGA TCAGTTTAGA AGAAATGAAG AAACATAACC AGGGAAAGGA GACTGCCCAA 
AGGGTACTAT CCCAAATTGA TGTGGCACAG AAAAAATTGC AGGATGTTTC CATGAAGTTT 
CGATTATTCC AGAAGCCAGC CAATTTTGAG CAGCGCCTAC AAGAAAGTAA AATGATTTTA 
GATGAAGTGA AGATGCATTT ACCTGCGTTG GAAACAAAGA GTGTGGAACA GGAAGTAGTA 
CAGTCACAGT TAAATCATTG TGTGAACTTG TATAAAAGTC TGAGTGAAGT GAAGTCTGAA 
GTGGAAATGG TAATAAAAAC TGGACGTCAG ATTGTACAGA AGAAGCAGAC GGAAAACCCG 
AAAGAGCTTG ATGAAAGAGT TACAGCTTTG AAATTGCATT ATAATGAGCT GGGAGCAAAG 
GTGACAGAAA GAAAGCAACA GTTGGAAAAA TGCTTGAAAT TGTCCCGTAA GATGCGAAAG 
GAAATGAATG CCCTGACAGA ATGGCTGGCA GCGACAGATA TGGAACTGAC GAAGAGATCG 
GCAGTTGAAG GAATGCCTAG TAATTTGGAT TCTGAAGTTG CCTGGGGAAA GGCTACTCAG 
AAAGAGATTG AGAAACAGAA GGTTCACCTA AAGAGTGTCA CAGAGGTAGG AGAGGCCTTG 
AAAACGGTTT TGGGCAAGAA GGAAATGTTG GTGGAAGATA AACTGAGTCT TCTGAATAGT 
AACTGGATAG CCGTCACTTC CCGAGCAGAA GAGTGGTTAA ACCTTTTATT GGAATACCAG 
AAACACATGG AAAACTTTGA CCAGAATGTG GATTACATCA CAAACTGGAT CATTCAGGCT 
GATGCACTTT TGGATGAATC TGAGAAAAAG AAACCTCAGC AAAAAGAAGA CATACTTAAG 
CGTTTAAAGG CTGAAATGAA TGACATACGT CCAAAGGTGG ATTCTACACG TGACCAAGCA 
GCAAACCTGA TGGCAAACCG CGGCGACCAC TGCAGGAAAG TAGTAGAGCC CAAAATCTCA 
GAGCTCAACC ATCGATTTGC AGCCATTTCT CACAGAATTA AGACTGGAAA GGCCTCCATT 
CCTTTGAAGG AATTGGAGCA GTTTAACTCA GATATACAAA AATTGCTTGA ACCACTGGAG 
GCTGAAATTC AGCAGGGGGT GAATCTGAAA GAGGAAGACT TCAATAAAGA TATGAGTGAA 
GACAATGAGG GTACTGTAAA AGAATTGTTG CAAAGAGGAG ACAACTTACA ACAAAGAATC 
ACAGATGAGA GAAAGCGAGA GGAAATAAAG ATAAAACAAC AGCTGTTACA GACAAAACAT 
AATGCTCTCA AGGATTTGAG GTCTCAAAGA AGAAAAAAGG CTCTAGAAAT TTCTCACCAG 
TGGTATCAGT ACAAGAGGCA GGCTGATGAT CTCCTGAAAT GCTTGGATGA CATTGAAAAA 
AAATTAGCCA GCCTACCTGA ACCCAGAGAT GAAAGGAAAA TAAAGGAAAT TGATCGTGAA 
TTGCAGAAGA AGAAAGAGGA GCTGAATGCA GTGCGTAGGC AAGCTGAGGG CTTGTCTGAG 
GATGGGGCCG CAATGGCAGT GGAGCCAACT CAGATCCAGC TCAGCAAGCG CTGGCGGGAA 
ATTGAGAGCA AATTTGCTCA GTTTCGAAGA CTCAACTTTG CACAAATTCA CACTGTCCAT 
GAAGAGTCAG TGGTGGCGAT GACTGAAGAC ATGCCTTTGG AAATTTCTTA TGTGCCTTCT 
ACTTACCTGA CTGAGATCAC TCATGTCTCA CAAGCCCTAT CAGAAGTGGA AGAGCTTCTT 
AATGCTCCCG ACCTTTGTGC TCAAGATTTT GAAGATCTCT TTAAACAAGA GGAATCCTTG 
AAGAACATAA AAGACAGCCT GCAACAAATC TCAGGTCGGA TTGACATCAT TCACAATAAA 
AAGACAGCAG CATTGCACAG TGCCACTCCT GCAGAAAGGG CAAAGCTCCA GGAAGCTCTC 
TCACGGCTTG ATTTCCAATG GGAAAGAGTT AACAATATGT ACAAGGACCG ACAAGGGAGA 
TTTGACAGAT CTGTGGAAAA ATGGCGGCGG TTTCATTATG ATATGAAGAT ACTTAATCAA 
TGGCTAACAG AAGCTGAACA GTTTCTCAAA AAGACACAAA TTCCTGAGAA TTGGGAACAT 
GCCAAATACA AATGGTATCT TAAGGAACTC CAGGATGGCA TTGGACAGCG GCAAAGTGTT 
GTCAGGGTAT TGAATGCAAC TGGGGAAGAA ATAATTCAAC AGTCCTCAAA AACAGATGCC 
AGTATTCTCC AAGAAAAACT GGGAAGCCTG AATCTGCGGT GGCAGGAGGT CTGCAAACAG 
CTGGCAGAAA GAAAAAAGAG GCTAGAGGAA CAGAAGAATA TCTTGTCAGA ATTTCAAAGA 
GATGTAAATG AATTTGTTTT ATGGTTGGAA GAAGCGGATA ACGTTGCTAA TATTCCACTT 
GAACCTGGAA ATGAGCAGCA GCTAAAAGAA AAACTTGAAC AAGTCAAGTT ACTGGCAGAA 
GAGTTGCCCC TGCGCCAGGG AATTCTAAAA CAATTAAATG AAACTGGAGG AACAGTGCTT 
GTAAGTGCTC CCCTAAGCCC AGAAGAGCAA GATAAACTTG AAAATAAGCT CAAGCAGACA 
AATCTTCAGT GGATAAAGGT TTCTAGAAAT CTGCCTGAGA AGCAAGAAGA AATTGAGGCA 
CACGTAAAAG ACCTTGGACA GCTGGAAGAG CAGTTAAATC ATCTGCTTCT ATGGCTGTCT 
CCTATTAGGA ATCAGTTGGA AATTTACAAT CAGCCAAATC AAACAGGACC ATTTGACATC 
AAGGAAATTG AAGTAGCAGT TCAAGCTAAA CAGCCGGATG TGGAAGGGAT TTTGTCTAAA 
GGGCAGCATT TGTACAAGGA AAAACCAGCC ACTCAGCCAG CGAAGAGAAA GCTGGAAGAT 
CTCAGCTCTG ATTGGAAGGT GGTAACTCAG TTGCTTCAAG AGCTGCGGGC AAAGCAGCCT 
GGCCCAGCTC CTGGACTGAC CACTGTCAGA GCCCCTCCCA GTCAGACTGT TACTCTGGTG 
ACACAACCCG CGGTTACCAA GGAAACTGCC ATCTCCAAAC TAGAAATGCC ATCTTCATTG 
CTGTTGGAGG TACCTGCACT GGCAGATTTC AACCGAGCTT GGACAGAACT TACCGACTGG 
CTGTCTCTGC TTGATCGAGT TATAAAATCA CAGAGGGTGA TGGTGGGTGA TCTTGAAGAC 
ATTAACGAGA TGATCATCAA GCAGAAGGCA ACGCTGCAGG ATTTGGAACA GAGGCGCCCC 
CAGTTGGAAG AACTCATTAC CGCTGCCCAG AATTTGAAAA ACAAGACCAG CAATCAAGAG 
GCTAGAACAA TCATTACTGA TCGAATTGAA AGAATTCAGA GTCAGTGGGA TGAAGTACAG 
GAACATCTTC AGAACCGGAG GCAACAGTTG AATGAAATGT TAAAGGATTC CACACAATGG 
CTGGAAGCTA AAGAGGAGGC TGAGCAGGTG TTGGGGCAGG CCAGAGCCAA GCTTGAGTCA 
TGGAAGGAGG CTCCCTACAC AGTAGATGCA ATCCAAAAGA AAATCACAGA AACCAAGTTG 
GCCAAAGACC TCCGCCAGTG GCAGATAAAT GTAGATGTTG CAAATGATTT GGCACTGAAA 
CTTCTCCGAG ATTATTCTGC AGATGATACC AGAAAAGTAC ACATGATAAC AGAGAACATC 
AATGCCTCTT GGGCAAGCAT CCATAAAAGA TTGAGTGAGC GAGAGGCTGC TCTGGAAGAA 
ACCCACAGAT TACTGCAACA GTTCCCCTTG GACCTGGAGA AGTTCCTTGC CTGGCTTACA 
GAAGCCGAAA CAACTGCCAA CGTCCTGCAG GATGCCACCC ATAAGGAAAG GCTTCTAGAA 
GATTCCAAGG GAGTAAGAGA GCTGATGAAA CAATGGCAAG ATCTCCAAGG AGAAATCGAA 
GCTCACACAG ATATCTATCA CAACCTGGAC GAAAATGGCC AAAAAGTCCT GAGATCCCTG 
GAAGGTTCTG ACGATGCAGC CTTGTTGCAA AGACGTTTGG ATAACATGAA CTTCAAGTGG 
AGCGAACTTC GGAAAAAGTC TCTCAACATT AGGTCTCACT TGGAAGCCAG TTCTGACCAG 
TGGAAGCGTC TGCACCTTTC TCTTCAGGAA CTTCTGGTAT GGCTCCAGCT GAAAGATGAT 
GAGTTAAGCC GGCAGGCACC CATTGGAGGA GACTTTCCAG CGGTGCAGAA GCAGAATGAT 
GTACACAGGG CCTTCAAGAG GGAATTGAAA ACGAAAGAAC CTGTAATCAT GAGTACTCTT 
GAGACTGTAC GAATATTTCT GACAGAGCAG CCTTTAGAAG GACTAGAGAA ACTCTACCAG 
GAGCCCAGAG AGCTGCCTCC TGAAGAGAGA GCCCAGAATG TCACACGGCT CCTACGAAAG 
CAAGCTGAGG AGGTCAACAC TCAGTGGGAA AAACTGAACG TGCACTCTGC AGACTGGCAG 
AGAAAAATAG ACGAGGCCCT CGAAAGACTC CAGGAGCTTC AGGAAGCAAC AGATGAGCTG 
GATCTCAAAC TACGTCAGGC AGAGGTGATC AAAGGATCCT GGCAGCCTGT GGGTGATCTC 
CTCATTGACT CTCTCCAAGA TCACCTCGAA AAAGTCAAGG CGCTTCGAGG AGAAATTACA 
CCTCTGAAAG AGAATGTCAG CTACGTCAAT GACCTTGCTC GCCAACTCAC TACGTTGGGC 
ATTCAGCTGT CACCATATAA CCTCAACACT CTGGAAGACC TGAACACCAG ATGGAAGCTT 
CTGCAGGTGG CCATTGAGGA CCGCATCAGG CAGCTGCATG AAGCGCACAG GGACTTTGGA 
CCAGCCTCCC AGCACTTCCT TTCCACTTCT GTCCAGGGTC CCTGGGAGAG AGCCATCTCA 
CCAAACAAAG TGCCCTACTA TATCAACCAC GAGACCCAAA CAACTTGCTG GGACCATCCC 
AAAATGACAG AGCTCTACCA GTCTTTAGCT GACCTGAATA ATGTCAGGTT CTCAGCTTAC 
AGGACTGCCA TGAAACTCCG AAGACTGCAG AAGGCCCTTT GCTTGGATCT CTTGAGCCTA 
TCGGCTGCAT GCGATGCCTT GGACCAGCAC AACCTCAAGC AAAATGACCA GCCCATGGAT 
ATCCTGCAGG TCATTAACTG TCTGACCACT ATTTATGATC GCCTAGAGCA AGAGCACAAC 
AATCTGGTCA ACGTCCCTCT CTGCGTGGAT ATGTGTCTCA ATTGGCTGCT GAATGTTTAT 
GACACGGGAC GAACGGGGAG GATCCGGGTC CTGTCTTTTA AAACTGGCAT CATTTCTCTG 
TGTAAAGCCC ATTTGGAAGA CAAGTACAGA TACCTCTTCA AGCAAGTGGC AAGTTCGACA 
GGATTTTGTG ACCAGCGCAG GCTGGGCCTC CTCCTGCATG ACTCTATCCA GATCCCAAGA 
CAGTTGGGTG AAGTCGCATC GTTCGGGGGC AGTAACATTG AGCCGAGTGT CAGGAGCTGC 
TTCCAGTTTG CTAATAATAA GCCTGAGATC GAAGCGGCCC TCTTCCTAGA CTGGATGCGC 
CTGGAGCCCC AGTCCATGGT GTGGCTGCCT GTCCTGCACC GAGTGGCTGC CGCGGAAACT 
GCCAAGCACC AGGCCAAGTG CAACATCTGC AAGGAGTGTC CCATCATCGG ATTCAGGTAC 
AGGAGTCTAA AGCACTTTAA TTATGACATC TGCCAAAGTT GCTTTTTTTC TGGTCGAGTT 
GCAAAAGGCC ATAAAATGCA CTATCCCATG GTGGAATACT GCACTCCGAC TACATCGGGA 
GAAGATGTCC GTGACTTTGC CAAGGTACTA AAAAACAAAT TTCGAACCAA AAGGTATTTT 
GCGAAGCATC CCCGAATGGG CTACCTGCCA GTGCAGACTG TCTTAGAGGG GGACAACATG 
GAAACTCCTG TCACTCTGAT CAACTTCTGG CCGGTAGATT CTGCGCCTGC CTCGTCCCCT 
CAGCTTTCAC ACGATGATAC TCATTCACGC ATTGAGCATT ATGCTAGCAG GCTAGCAGAA 
ATGGAAAACA GCAATGGATC TTATCTAAAT GATAGCATCT CTCCTAATGA GAGCATAGAT 
GATGAACATT TGTTAATCCA GCATTACTGC CAAAGTTTGA ACCAGGACTC CCCCCTGAGC 
CAGCCTCGTA GTCCTGCCCA GATCTTGATT TCCTTAGAGA GTGAGGAAAG AGGGGAGCTA 
GAGAGAATCC TAGCAGATCT TGAGGAAGAA AACAGAAATC TGCAAGCAGA GTATGATCGT 
CTAAAGCAGC AGCATGAACA CAAAGGCCTG TCCCCACTGC CATCCCCTCC TGAAATGATG 
CCTACTTCTC CCCAAAGTCC CCGGGATGCT GAGCTCATCG CTGAGGCCAA GCTGCTGCGT 
CAACACAAAG GCCGCCTGGA AGCCAGGATG CAAATCCTTG AAGACCATAA CAAACAACTG 
GAATCCCAGT TACACAGGCT CAGGCAGCTG CTGGAGCAAC CCCAGGCAGA GGCCAAGGTG 
AATGGTACAA CGGTGTCTTC TCCTTCTACC TCTCTTCAGA GGTCAGATAG CAGTCAGCCT 
ATGCTGCTCC GGGTGGTCGG CAGTCAGACT TCAGAATCCA TGGGCGAGGA AGACCTGCTC 
AGCCCTCCCC AGGACACAAG CACAGGGTTA GAGGAAGTGA TGGAGCAGCT CAACCACTCC 
TTCCCTAGTT CCAGAGGAAG AAATACCCCT GGGAAGCCAA TGAGAGAGGA CACAATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMD ( XM_014111345.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013966820.1 Canis lupus familiaris dystrophin (DMD), transcript variant X6, mRNA.

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CloneID	OCe45416
Clone ID Related Accession (Same CDS sequence)	XM_014111345.2 , XM_014111345.1
Accession Version	XM_014111345.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	dystrophin isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTGGT GGGAAGAAGT AGAGGACTGT TATGAAAGAG AAGATGTTCA AAAGAAAACA 
TTCACAAAAT GGATAAATGC ACAGTTTTCT AAGTTTGGGA AGCAGCACAT AGAGAACCTC 
TTCAGTGACC TACAGGATGG GAGACGCCTC CTAGACCTTT TGGAAGGCCT GACAGGGCAA 
AAACTGCCAA AAGAAAAAGG ATCCACAAGA GTTCATGCCC TGAACAATGT CAACAAGGCA 
CTGCGCGTCT TGCAGAAAAA TAATGTTGAT TTAGTGAACA TTGGAAGTAC TGACATAGTA 
GATGGAAATC ACAAACTGAC TCTTGGTTTG ATTTGGAATA TAATCCTCCA CTGGCAGGTC 
AAAAATGTAA TGAAAAATAT CATGGCTGGA TTGCAACAAA CCAACAGTGA AAAGATTCTC 
CTGAGCTGGG TCCGACAATC AACTCGTAAT TATCCACAGG TTAATGTCAT TAACTTCACC 
ACCAGCTGGT CTGATGGCCT GGCTTTGAAC GCTCTCATCC ACAGTCATAG GCCAGACCTG 
TTTGATTGGA ATAGTGTGGT TTGCCAGCAG TCAGCCACAC AACGCCTGGA ACATGCATTC 
AACATTGCCA AATATCAATT AGGCATAGAG AAACTGCTTG ATCCTGAAGA TGTTGCCACC 
ACTTATCCAG ATAAGAAGTC CATCTTAATG TATATCACAT CACTCTTCCA AGTTTTGCCT 
CAACAAGTGA GCATTGAAGC CATCCAGGAA GTGGAAATGT TGCCAAGGCC ATCAAAAGTT 
ACTAGAGAAG AACATTTTCA ATTACATCAT CAAATGCACT ATTCTCAACA GATCACAGTC 
AGTCTAGCAC AGGGCTATGA ACGAGCCCCT TCCTCTCCTA AGCCTCGGTT CAAGAGCTAT 
GCCTACACAC AGGCTGCTTA TGTCACCACT TCTGACCCCA CACGGAGCCC ACTTCCTTCA 
CAGCATTTGG AAACTCCTGA AGACAAGTCA TTTGGCCGGT CATTGACAGA GACCGAAGCA 
AACCTGGACA GTTATCAAAC AGCTTTGGAA GAAGTACTCT CGTGGCTTCT TTCAGCTGAG 
GATGCACTGC AAGCCCAAGG AGAGATTTCT AATGATGTCG AAGAAGTGAA AGAACAATTT 
CATACTCATG AGGGATATAT GATGGACTTG ACATCCCATC AGGGACGGGT CGGTAATGTT 
CTCCAACTGG GAAGTCAACT GATTGGAACA GGGAAATTAT CAGAAGATGA AGAAACCGAA 
GTGCAGGAAC AAATGAATCT CCTCAATTCA AGATGGGAAT GCCTCAGGGT AGCTAGCATG 
GAAAAACAAA GCAATTTACA TAAAGTTCTA ATGGATCTCC AGAATCAGCA ACTGAAAGAG 
TTAAATGACT GGCTAACCAA AACAGAAGAG AGAACAAGGA AAATGGAGAA GGAGCCCCTT 
GGACCTGATA TTGAAGACCT AAAACGCCAA GTACAACAAC ATAAGGTGCT TCAAGAAGAC 
TTAGAACAGG AACAAGTCAG GGTCAATTCC CTCACTCATA TGGTGGTGGT AGTCGATGAA 
TCTAGTGGAG ACCATGCAAC TGCTGCTTTG GAAGAACAAC TTAAGGTACT GGGAGATCGA 
TGGGCAAACA TCTGTAGGTG GACAGAAGAT CGCTGGGTTC TTTTACAAGA CATCCTCCTA 
AAATGGCAGC GTTTTACTGA AGAACAGTGC CTTTTCAGTG CATGGCTTTC GGAGAAGGAA 
GATGCAGTGA ACAAGATTCA CACAACTGGC TTTAAGGATC AAAGTGAAGT GTTATCAAAT 
CTTCAGAAAC TGGCTGTCTT AAAAACAGAT CTGGAAAAGA AGAAGCAAAC CATGGACAAA 
CTCTGCTCAC TCAACCAAGA CCTTCTTTCA GCGCTGAAAA ACACAGTGGT AGCCCACAAG 
ATGGAAGCAT GGCTGGACAA CTTTGCCCAG CGCTGGGATA ATTTAGTCCA GAAACTTGAA 
AAAAGTTCAG CACAGATTTC ACAGGCTGTC ACCACCACTC AGCCATCACT AACACAGACA 
ACTGTAATGG AAACAGTAAC TATGGTGACC ACGAGGGAAC ACATCTTGGT AAAGCATGCC 
CAAGAGGAAC TGCCACCACC ACCCCCTCAG AAGAAGAGGC AGATTATCGT GGATTCTGAA 
ATTAGGAAAA GGTTGGATGT CGATATAACT GAACTTCACA GTTGGATTAC TCGTTCAGAA 
GCTGTGTTGC AGAGTCCTGA ATTTGCAATC TATCGGAAGG AAGGCAACTT CTCAGACCTT 
AAAGAAAAAG TCAATGCCAT AGAGCGAGAA AAAGCCGAGA AGTTCAGAAA ACTGCAAGAT 
GCCAGCAGAT CAGCTCAGGC CCTGGTGGAA CAGATGGTGA ATGAGGGTGT TAATGCTGAC 
AGCATCAAAC AAGCCTCCGA ACAACTGAAC AGCCGGTGGA TAGAGTTCTG CCAATTGCTA 
AGCGAGAGAC TTAACTGGCT GGAGTATCAG AACAACATCA TCACTTTCTA TAATCAGCTA 
CAACAATTGG AGCAGATGAC AACTACTGCT GAAAACTGGT TGAAAACCCA GCCTACCACC 
ACATCAGAGC CAACAGCAAT TAAAAGCCAG TTAAAAATTT GTAAGGATGA AATCAACCGA 
CTGTCAGCTC TTCAGCCTCA AATCGAGCGA TTAAAAATTC AAAGCATAGC CCTGAAAGAG 
AAAGGACAAG GGCCAATGTT CCTGGATGCA GACTTTGTGG CCTTTACAAA TCATTTTAAC 
CAAGTCTTTG CTGATGTGCA GGCAAGAGAA AAAGAGCTAC AAACAATTTT TGACAGTTTG 
CCACCCATGC GCTATCAGGA GACTATGAGT ACCATCCTGA CATGGATCCA GCAGTCAGAA 
ACCAAACTCT CTATACCTCA GGTTACTGTC ACTGAATATG ACATCATGGA ACAGAGACTC 
GGAGAGCTAC AGGCTTTACA AAGCTCTCTG CAAGAGCAAC AAAATGGCCT AAACTATCTC 
AGCACCACTG TGAAAGAGAT GTCAAAGAAA GCACCACTGT CTGATATTAG TCGGAAATAT 
CAATCAGAAT TTGAAGAGAT TGAGGGACGT TGGAAGAAGC TGTCTTCCCA GCTGGTTGAA 
CATTGTCAAA AGTTGGAGGA GCAAATGGCT AAACTTCGAA AAATTCAGAA TCACATAAAA 
ACTCTGAAGA AATGGATCAC TGAAGTCGAT GTTTTCCTGA AGGAGGAATG GCCTGCCCTT 
GGGGATTCAG AAATTCTGAA AAGACAGCTG AAACAGTGCA GGCTTTTAGT CAATGACATT 
CAGACCATCC AGCCTAGTCT CAACAGTGTC AATGAAGGGG CTCAGAAGAT GAAGAATGAA 
GCAGAACCAG AGTTTGCTGG CAGACTTGAG ACAGAGCTCC GAGAACTTAA CACCCAGTGG 
GATTACATGT GCCGCCAGGT CTATGCCAGG AAGGAAGCCT TAAAAGGAGG TTTGGATAAA 
ACTGTAAGTC TTCAGAAAGA TCTGTCAGAG ATGCATGAGT GGATGACACA AGCTGAAGAA 
GAATACCTAG AGAGAGATTT CGAATACAAG ACCCCTGATG AATTACAGAC AGCAGTTGAA 
GAGATGAAGA GAGCTAAAGA AGAGGCCCAG CAAAAAGAAG CAAAAGTGAA ACTCCTAACC 
GAGTCCGTCA ATAGTGTCAT AGCTCAGGCT CCACCTGCAG CACAAGAGGC CTTAAAAAAG 
GAACTTGACA CTCTCACCAC CAACTACCAG TGGCTCTGCA CCAGGCTCAA TGGCAAATGC 
AAGACCTTGG AAGAAGTTTG GGCGTGCTGG CATGAGTTAT TGTCCTACTT GGAGAAGGCA 
AACAAGTGGC TAAGTGAAGT AGAAGTCAAG CTTAAAACCA CTGAAAATAT TTCTGGGGGA 
GCTGAGGAAA TCGCCGAGGT GCTTGATTCG CTTGAAAATT TGATGCAACA TTCAGAGGAT 
AACCCGAATC AGATTCGCAT ATTGGCACAG ACCTTGACAG ATGGTGGAGT CATGGATGAA 
CTGATCAATG AGGAGCTTGA GACATTTAAT TCTCGTTGGA GAGAACTCCA TGAAGAGGCT 
GTGAGGAGGC AAAAGTTGCT TGAGCAGAGT ATCCAGTCGG CCCAGGAGAT AGAAAAATCC 
TTGCACTTAA TTCAGGAGTC CCTCTCTTCC ATTGACAAGC AGTTGGCAGC TTATATTGCT 
GACAAAGTGG ATGCAGCTCA GATGCCTCAG GAAGCCCAGA AAATCCAATC AGATTTGACA 
AGTCATGAGA TCAGTTTAGA AGAAATGAAG AAACATAACC AGGGAAAGGA GACTGCCCAA 
AGGGTACTAT CCCAAATTGA TGTGGCACAG AAAAAATTGC AGGATGTTTC CATGAAGTTT 
CGATTATTCC AGAAGCCAGC CAATTTTGAG CAGCGCCTAC AAGAAAGTAA AATGATTTTA 
GATGAAGTGA AGATGCATTT ACCTGCGTTG GAAACAAAGA GTGTGGAACA GGAAGTAGTA 
CAGTCACAGT TAAATCATTG TGTGAACTTG TATAAAAGTC TGAGTGAAGT GAAGTCTGAA 
GTGGAAATGG TAATAAAAAC TGGACGTCAG ATTGTACAGA AGAAGCAGAC GGAAAACCCG 
AAAGAGCTTG ATGAAAGAGT TACAGCTTTG AAATTGCATT ATAATGAGCT GGGAGCAAAG 
GTGACAGAAA GAAAGCAACA GTTGGAAAAA TGCTTGAAAT TGTCCCGTAA GATGCGAAAG 
GAAATGAATG CCCTGACAGA ATGGCTGGCA GCGACAGATA TGGAACTGAC GAAGAGATCG 
GCAGTTGAAG GAATGCCTAG TAATTTGGAT TCTGAAGTTG CCTGGGGAAA GGCTACTCAG 
AAAGAGATTG AGAAACAGAA GGTTCACCTA AAGAGTGTCA CAGAGGTAGG AGAGGCCTTG 
AAAACGGTTT TGGGCAAGAA GGAAATGTTG GTGGAAGATA AACTGAGTCT TCTGAATAGT 
AACTGGATAG CCGTCACTTC CCGAGCAGAA GAGTGGTTAA ACCTTTTATT GGAATACCAG 
AAACACATGG AAAACTTTGA CCAGAATGTG GATTACATCA CAAACTGGAT CATTCAGGCT 
GATGCACTTT TGGATGAATC TGAGAAAAAG AAACCTCAGC AAAAAGAAGA CATACTTAAG 
CGTTTAAAGG CTGAAATGAA TGACATACGT CCAAAGGTGG ATTCTACACG TGACCAAGCA 
GCAAACCTGA TGGCAAACCG CGGCGACCAC TGCAGGAAAG TAGTAGAGCC CAAAATCTCA 
GAGCTCAACC ATCGATTTGC AGCCATTTCT CACAGAATTA AGACTGGAAA GGCCTCCATT 
CCTTTGAAGG AATTGGAGCA GTTTAACTCA GATATACAAA AATTGCTTGA ACCACTGGAG 
GCTGAAATTC AGCAGGGGGT GAATCTGAAA GAGGAAGACT TCAATAAAGA TATGAGTGAA 
GACAATGAGG GTACTGTAAA AGAATTGTTG CAAAGAGGAG ACAACTTACA ACAAAGAATC 
ACAGATGAGA GAAAGCGAGA GGAAATAAAG ATAAAACAAC AGCTGTTACA GACAAAACAT 
AATGCTCTCA AGGATTTGAG GTCTCAAAGA AGAAAAAAGG CTCTAGAAAT TTCTCACCAG 
TGGTATCAGT ACAAGAGGCA GGCTGATGAT CTCCTGAAAT GCTTGGATGA CATTGAAAAA 
AAATTAGCCA GCCTACCTGA ACCCAGAGAT GAAAGGAAAA TAAAGGAAAT TGATCGTGAA 
TTGCAGAAGA AGAAAGAGGA GCTGAATGCA GTGCGTAGGC AAGCTGAGGG CTTGTCTGAG 
GATGGGGCCG CAATGGCAGT GGAGCCAACT CAGATCCAGC TCAGCAAGCG CTGGCGGGAA 
ATTGAGAGCA AATTTGCTCA GTTTCGAAGA CTCAACTTTG CACAAATTCA CACTGTCCAT 
GAAGAGTCAG TGGTGGCGAT GACTGAAGAC ATGCCTTTGG AAATTTCTTA TGTGCCTTCT 
ACTTACCTGA CTGAGATCAC TCATGTCTCA CAAGCCCTAT CAGAAGTGGA AGAGCTTCTT 
AATGCTCCCG ACCTTTGTGC TCAAGATTTT GAAGATCTCT TTAAACAAGA GGAATCCTTG 
AAGAACATAA AAGACAGCCT GCAACAAATC TCAGGTCGGA TTGACATCAT TCACAATAAA 
AAGACAGCAG CATTGCACAG TGCCACTCCT GCAGAAAGGG CAAAGCTCCA GGAAGCTCTC 
TCACGGCTTG ATTTCCAATG GGAAAGAGTT AACAATATGT ACAAGGACCG ACAAGGGAGA 
TTTGACAGAT CTGTGGAAAA ATGGCGGCGG TTTCATTATG ATATGAAGAT ACTTAATCAA 
TGGCTAACAG AAGCTGAACA GTTTCTCAAA AAGACACAAA TTCCTGAGAA TTGGGAACAT 
GCCAAATACA AATGGTATCT TAAGGAACTC CAGGATGGCA TTGGACAGCG GCAAAGTGTT 
GTCAGGGTAT TGAATGCAAC TGGGGAAGAA ATAATTCAAC AGTCCTCAAA AACAGATGCC 
AGTATTCTCC AAGAAAAACT GGGAAGCCTG AATCTGCGGT GGCAGGAGGT CTGCAAACAG 
CTGGCAGAAA GAAAAAAGAG GCTAGAGGAA CAGAAGAATA TCTTGTCAGA ATTTCAAAGA 
GATGTAAATG AATTTGTTTT ATGGTTGGAA GAAGCGGATA ACGTTGCTAA TATTCCACTT 
GAACCTGGAA ATGAGCAGCA GCTAAAAGAA AAACTTGAAC AAGTCAAGTT ACTGGCAGAA 
GAGTTGCCCC TGCGCCAGGG AATTCTAAAA CAATTAAATG AAACTGGAGG AACAGTGCTT 
GTAAGTGCTC CCCTAAGCCC AGAAGAGCAA GATAAACTTG AAAATAAGCT CAAGCAGACA 
AATCTTCAGT GGATAAAGGT TTCTAGAAAT CTGCCTGAGA AGCAAGAAGA AATTGAGGCA 
CACGTAAAAG ACCTTGGACA GCTGGAAGAG CAGTTAAATC ATCTGCTTCT ATGGCTGTCT 
CCTATTAGGA ATCAGTTGGA AATTTACAAT CAGCCAAATC AAACAGGACC ATTTGACATC 
AAGGAAATTG AAGTAGCAGT TCAAGCTAAA CAGCCGGATG TGGAAGGGAT TTTGTCTAAA 
GGGCAGCATT TGTACAAGGA AAAACCAGCC ACTCAGCCAG CGAAGAGAAA GCTGGAAGAT 
CTCAGCTCTG ATTGGAAGGT GGTAACTCAG TTGCTTCAAG AGCTGCGGGC AAAGCAGCCT 
GGCCCAGCTC CTGGACTGAC CACTGTCAGA GCCCCTCCCA GTCAGACTGT TACTCTGGTG 
ACACAACCCG CGGTTACCAA GGAAACTGCC ATCTCCAAAC TAGAAATGCC ATCTTCATTG 
CTGTTGGAGG TACCTGCACT GGCAGATTTC AACCGAGCTT GGACAGAACT TACCGACTGG 
CTGTCTCTGC TTGATCGAGT TATAAAATCA CAGAGGGTGA TGGTGGGTGA TCTTGAAGAC 
ATTAACGAGA TGATCATCAA GCAGAAGGCA ACGCTGCAGG ATTTGGAACA GAGGCGCCCC 
CAGTTGGAAG AACTCATTAC CGCTGCCCAG AATTTGAAAA ACAAGACCAG CAATCAAGAG 
GCTAGAACAA TCATTACTGA TCGAATTGAA AGAATTCAGA GTCAGTGGGA TGAAGTACAG 
GAACATCTTC AGAACCGGAG GCAACAGTTG AATGAAATGT TAAAGGATTC CACACAATGG 
CTGGAAGCTA AAGAGGAGGC TGAGCAGGTG TTGGGGCAGG CCAGAGCCAA GCTTGAGTCA 
TGGAAGGAGG CTCCCTACAC AGTAGATGCA ATCCAAAAGA AAATCACAGA AACCAAGTTG 
GCCAAAGACC TCCGCCAGTG GCAGATAAAT GTAGATGTTG CAAATGATTT GGCACTGAAA 
CTTCTCCGAG ATTATTCTGC AGATGATACC AGAAAAGTAC ACATGATAAC AGAGAACATC 
AATGCCTCTT GGGCAAGCAT CCATAAAAGA TTGAGTGAGC GAGAGGCTGC TCTGGAAGAA 
ACCCACAGAT TACTGCAACA GTTCCCCTTG GACCTGGAGA AGTTCCTTGC CTGGCTTACA 
GAAGCCGAAA CAACTGCCAA CGTCCTGCAG GATGCCACCC ATAAGGAAAG GCTTCTAGAA 
GATTCCAAGG GAGTAAGAGA GCTGATGAAA CAATGGCAAG ATCTCCAAGG AGAAATCGAA 
GCTCACACAG ATATCTATCA CAACCTGGAC GAAAATGGCC AAAAAGTCCT GAGATCCCTG 
GAAGGTTCTG ACGATGCAGC CTTGTTGCAA AGACGTTTGG ATAACATGAA CTTCAAGTGG 
AGCGAACTTC GGAAAAAGTC TCTCAACATT AGGTCTCACT TGGAAGCCAG TTCTGACCAG 
TGGAAGCGTC TGCACCTTTC TCTTCAGGAA CTTCTGGTAT GGCTCCAGCT GAAAGATGAT 
GAGTTAAGCC GGCAGGCACC CATTGGAGGA GACTTTCCAG CGGTGCAGAA GCAGAATGAT 
GTACACAGGG CCTTCAAGAG GGAATTGAAA ACGAAAGAAC CTGTAATCAT GAGTACTCTT 
GAGACTGTAC GAATATTTCT GACAGAGCAG CCTTTAGAAG GACTAGAGAA ACTCTACCAG 
GAGCCCAGAG AGCTGCCTCC TGAAGAGAGA GCCCAGAATG TCACACGGCT CCTACGAAAG 
CAAGCTGAGG AGGTCAACAC TCAGTGGGAA AAACTGAACG TGCACTCTGC AGACTGGCAG 
AGAAAAATAG ACGAGGCCCT CGAAAGACTC CAGGAGCTTC AGGAAGCAAC AGATGAGCTG 
GATCTCAAAC TACGTCAGGC AGAGGTGATC AAAGGATCCT GGCAGCCTGT GGGTGATCTC 
CTCATTGACT CTCTCCAAGA TCACCTCGAA AAAGTCAAGG CGCTTCGAGG AGAAATTACA 
CCTCTGAAAG AGAATGTCAG CTACGTCAAT GACCTTGCTC GCCAACTCAC TACGTTGGGC 
ATTCAGCTGT CACCATATAA CCTCAACACT CTGGAAGACC TGAACACCAG ATGGAAGCTT 
CTGCAGGTGG CCATTGAGGA CCGCATCAGG CAGCTGCATG AAGCGCACAG GGACTTTGGA 
CCAGCCTCCC AGCACTTCCT TTCCACTTCT GTCCAGGGTC CCTGGGAGAG AGCCATCTCA 
CCAAACAAAG TGCCCTACTA TATCAACCAC GAGACCCAAA CAACTTGCTG GGACCATCCC 
AAAATGACAG AGCTCTACCA GTCTTTAGCT GACCTGAATA ATGTCAGGTT CTCAGCTTAC 
AGGACTGCCA TGAAACTCCG AAGACTGCAG AAGGCCCTTT GCTTGGATCT CTTGAGCCTA 
TCGGCTGCAT GCGATGCCTT GGACCAGCAC AACCTCAAGC AAAATGACCA GCCCATGGAT 
ATCCTGCAGG TCATTAACTG TCTGACCACT ATTTATGATC GCCTAGAGCA AGAGCACAAC 
AATCTGGTCA ACGTCCCTCT CTGCGTGGAT ATGTGTCTCA ATTGGCTGCT GAATGTTTAT 
GACACGGGAC GAACGGGGAG GATCCGGGTC CTGTCTTTTA AAACTGGCAT CATTTCTCTG 
TGTAAAGCCC ATTTGGAAGA CAAGTACAGA TACCTCTTCA AGCAAGTGGC AAGTTCGACA 
GGATTTTGTG ACCAGCGCAG GCTGGGCCTC CTCCTGCATG ACTCTATCCA GATCCCAAGA 
CAGTTGGGTG AAGTCGCATC GTTCGGGGGC AGTAACATTG AGCCGAGTGT CAGGAGCTGC 
TTCCAGTTTG CTAATAATAA GCCTGAGATC GAAGCGGCCC TCTTCCTAGA CTGGATGCGC 
CTGGAGCCCC AGTCCATGGT GTGGCTGCCT GTCCTGCACC GAGTGGCTGC CGCGGAAACT 
GCCAAGCACC AGGCCAAGTG CAACATCTGC AAGGAGTGTC CCATCATCGG ATTCAGGTAC 
AGGAGTCTAA AGCACTTTAA TTATGACATC TGCCAAAGTT GCTTTTTTTC TGGTCGAGTT 
GCAAAAGGCC ATAAAATGCA CTATCCCATG GTGGAATACT GCACTCCGAC TACATCGGGA 
GAAGATGTCC GTGACTTTGC CAAGGTACTA AAAAACAAAT TTCGAACCAA AAGGTATTTT 
GCGAAGCATC CCCGAATGGG CTACCTGCCA GTGCAGACTG TCTTAGAGGG GGACAACATG 
GAAACTCCTG TCACTCTGAT CAACTTCTGG CCGGTAGATT CTGCGCCTGC CTCGTCCCCT 
CAGCTTTCAC ACGATGATAC TCATTCACGC ATTGAGCATT ATGCTAGCAG GCTAGCAGAA 
ATGGAAAACA GCAATGGATC TTATCTAAAT GATAGCATCT CTCCTAATGA GAGCATAGAT 
GATGAACATT TGTTAATCCA GCATTACTGC CAAAGTTTGA ACCAGGACTC CCCCCTGAGC 
CAGCCTCGTA GTCCTGCCCA GATCTTGATT TCCTTAGAGA GTGAGGAAAG AGGGGAGCTA 
GAGAGAATCC TAGCAGATCT TGAGGAAGAA AACAGAAATC TGCAAGCAGA GTATGATCGT 
CTAAAGCAGC AGCATGAACA CAAAGGCCTG TCCCCACTGC CATCCCCTCC TGAAATGATG 
CCTACTTCTC CCCAAAGTCC CCGGGATGCT GAGCTCATCG CTGAGGCCAA GCTGCTGCGT 
CAACACAAAG GCCGCCTGGA AGCCAGGATG CAAATCCTTG AAGACCATAA CAAACAACTG 
GAATCCCAGT TACACAGGCT CAGGCAGCTG CTGGAGCAAC CCCAGGCAGA GGCCAAGGTG 
AATGGTACAA CGGTGTCTTC TCCTTCTACC TCTCTTCAGA GGTCAGATAG CAGTCAGCCT 
ATGCTGCTCC GGGTGGTCGG CAGTCAGACT TCAGAATCCA TGGGCGAGGA AGACCTGCTC 
AGCCCTCCCC AGGACACAAG CACAGGGTTA GAGGAAGTGA TGGAGCAGCT CAACCACTCC 
TTCCCTAGTT CCAGAGGAAG AAATACCCCT GGGAAGCCAA TGAGAGAGGA CACAATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013966820.1
CDS	248..11287
Translation	MLWWEEVEDCYEREDVQKKTFTKWINAQFSKFGKQHIENLFSDLQDGRRLLDLLEGLTGQKLPKEKGSTRVHALNNVNKALRVLQKNNVDLVNIGSTDIVDGNHKLTLGLIWNIILHWQVKNVMKNIMAGLQQTNSEKILLSWVRQSTRNYPQVNVINFTTSWSDGLALNALIHSHRPDLFDWNSVVCQQSATQRLEHAFNIAKYQLGIEKLLDPEDVATTYPDKKSILMYITSLFQVLPQQVSIEAIQEVEMLPRPSKVTREEHFQLHHQMHYSQQITVSLAQGYERAPSSPKPRFKSYAYTQAAYVTTSDPTRSPLPSQHLETPEDKSFGRSLTETEANLDSYQTALEEVLSWLLSAEDALQAQGEISNDVEEVKEQFHTHEGYMMDLTSHQGRVGNVLQLGSQLIGTGKLSEDEETEVQEQMNLLNSRWECLRVASMEKQSNLHKVLMDLQNQQLKELNDWLTKTEERTRKMEKEPLGPDIEDLKRQVQQHKVLQEDLEQEQVRVNSLTHMVVVVDESSGDHATAALEEQLKVLGDRWANICRWTEDRWVLLQDILLKWQRFTEEQCLFSAWLSEKEDAVNKIHTTGFKDQSEVLSNLQKLAVLKTDLEKKKQTMDKLCSLNQDLLSALKNTVVAHKMEAWLDNFAQRWDNLVQKLEKSSAQISQAVTTTQPSLTQTTVMETVTMVTTREHILVKHAQEELPPPPPQKKRQIIVDSEIRKRLDVDITELHSWITRSEAVLQSPEFAIYRKEGNFSDLKEKVNAIEREKAEKFRKLQDASRSAQALVEQMVNEGVNADSIKQASEQLNSRWIEFCQLLSERLNWLEYQNNIITFYNQLQQLEQMTTTAENWLKTQPTTTSEPTAIKSQLKICKDEINRLSALQPQIERLKIQSIALKEKGQGPMFLDADFVAFTNHFNQVFADVQAREKELQTIFDSLPPMRYQETMSTILTWIQQSETKLSIPQVTVTEYDIMEQRLGELQALQSSLQEQQNGLNYLSTTVKEMSKKAPLSDISRKYQSEFEEIEGRWKKLSSQLVEHCQKLEEQMAKLRKIQNHIKTLKKWITEVDVFLKEEWPALGDSEILKRQLKQCRLLVNDIQTIQPSLNSVNEGAQKMKNEAEPEFAGRLETELRELNTQWDYMCRQVYARKEALKGGLDKTVSLQKDLSEMHEWMTQAEEEYLERDFEYKTPDELQTAVEEMKRAKEEAQQKEAKVKLLTESVNSVIAQAPPAAQEALKKELDTLTTNYQWLCTRLNGKCKTLEEVWACWHELLSYLEKANKWLSEVEVKLKTTENISGGAEEIAEVLDSLENLMQHSEDNPNQIRILAQTLTDGGVMDELINEELETFNSRWRELHEEAVRRQKLLEQSIQSAQEIEKSLHLIQESLSSIDKQLAAYIADKVDAAQMPQEAQKIQSDLTSHEISLEEMKKHNQGKETAQRVLSQIDVAQKKLQDVSMKFRLFQKPANFEQRLQESKMILDEVKMHLPALETKSVEQEVVQSQLNHCVNLYKSLSEVKSEVEMVIKTGRQIVQKKQTENPKELDERVTALKLHYNELGAKVTERKQQLEKCLKLSRKMRKEMNALTEWLAATDMELTKRSAVEGMPSNLDSEVAWGKATQKEIEKQKVHLKSVTEVGEALKTVLGKKEMLVEDKLSLLNSNWIAVTSRAEEWLNLLLEYQKHMENFDQNVDYITNWIIQADALLDESEKKKPQQKEDILKRLKAEMNDIRPKVDSTRDQAANLMANRGDHCRKVVEPKISELNHRFAAISHRIKTGKASIPLKELEQFNSDIQKLLEPLEAEIQQGVNLKEEDFNKDMSEDNEGTVKELLQRGDNLQQRITDERKREEIKIKQQLLQTKHNALKDLRSQRRKKALEISHQWYQYKRQADDLLKCLDDIEKKLASLPEPRDERKIKEIDRELQKKKEELNAVRRQAEGLSEDGAAMAVEPTQIQLSKRWREIESKFAQFRRLNFAQIHTVHEESVVAMTEDMPLEISYVPSTYLTEITHVSQALSEVEELLNAPDLCAQDFEDLFKQEESLKNIKDSLQQISGRIDIIHNKKTAALHSATPAERAKLQEALSRLDFQWERVNNMYKDRQGRFDRSVEKWRRFHYDMKILNQWLTEAEQFLKKTQIPENWEHAKYKWYLKELQDGIGQRQSVVRVLNATGEEIIQQSSKTDASILQEKLGSLNLRWQEVCKQLAERKKRLEEQKNILSEFQRDVNEFVLWLEEADNVANIPLEPGNEQQLKEKLEQVKLLAEELPLRQGILKQLNETGGTVLVSAPLSPEEQDKLENKLKQTNLQWIKVSRNLPEKQEEIEAHVKDLGQLEEQLNHLLLWLSPIRNQLEIYNQPNQTGPFDIKEIEVAVQAKQPDVEGILSKGQHLYKEKPATQPAKRKLEDLSSDWKVVTQLLQELRAKQPGPAPGLTTVRAPPSQTVTLVTQPAVTKETAISKLEMPSSLLLEVPALADFNRAWTELTDWLSLLDRVIKSQRVMVGDLEDINEMIIKQKATLQDLEQRRPQLEELITAAQNLKNKTSNQEARTIITDRIERIQSQWDEVQEHLQNRRQQLNEMLKDSTQWLEAKEEAEQVLGQARAKLESWKEAPYTVDAIQKKITETKLAKDLRQWQINVDVANDLALKLLRDYSADDTRKVHMITENINASWASIHKRLSEREAALEETHRLLQQFPLDLEKFLAWLTEAETTANVLQDATHKERLLEDSKGVRELMKQWQDLQGEIEAHTDIYHNLDENGQKVLRSLEGSDDAALLQRRLDNMNFKWSELRKKSLNIRSHLEASSDQWKRLHLSLQELLVWLQLKDDELSRQAPIGGDFPAVQKQNDVHRAFKRELKTKEPVIMSTLETVRIFLTEQPLEGLEKLYQEPRELPPEERAQNVTRLLRKQAEEVNTQWEKLNVHSADWQRKIDEALERLQELQEATDELDLKLRQAEVIKGSWQPVGDLLIDSLQDHLEKVKALRGEITPLKENVSYVNDLARQLTTLGIQLSPYNLNTLEDLNTRWKLLQVAIEDRIRQLHEAHRDFGPASQHFLSTSVQGPWERAISPNKVPYYINHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQVINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPVTLINFWPVDSAPASSPQLSHDDTHSRIEHYASRLAEMENSNGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKQQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDTSTGLEEVMEQLNHSFPSSRGRNTPGKPMREDTM

Target ORF information:

RefSeq Version	XM_014111345.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris dystrophin (DMD), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111345.1

ORF Insert Sequence:

ATGCTTTGGT GGGAAGAAGT AGAGGACTGT TATGAAAGAG AAGATGTTCA AAAGAAAACA 
TTCACAAAAT GGATAAATGC ACAGTTTTCT AAGTTTGGGA AGCAGCACAT AGAGAACCTC 
TTCAGTGACC TACAGGATGG GAGACGCCTC CTAGACCTTT TGGAAGGCCT GACAGGGCAA 
AAACTGCCAA AAGAAAAAGG ATCCACAAGA GTTCATGCCC TGAACAATGT CAACAAGGCA 
CTGCGCGTCT TGCAGAAAAA TAATGTTGAT TTAGTGAACA TTGGAAGTAC TGACATAGTA 
GATGGAAATC ACAAACTGAC TCTTGGTTTG ATTTGGAATA TAATCCTCCA CTGGCAGGTC 
AAAAATGTAA TGAAAAATAT CATGGCTGGA TTGCAACAAA CCAACAGTGA AAAGATTCTC 
CTGAGCTGGG TCCGACAATC AACTCGTAAT TATCCACAGG TTAATGTCAT TAACTTCACC 
ACCAGCTGGT CTGATGGCCT GGCTTTGAAC GCTCTCATCC ACAGTCATAG GCCAGACCTG 
TTTGATTGGA ATAGTGTGGT TTGCCAGCAG TCAGCCACAC AACGCCTGGA ACATGCATTC 
AACATTGCCA AATATCAATT AGGCATAGAG AAACTGCTTG ATCCTGAAGA TGTTGCCACC 
ACTTATCCAG ATAAGAAGTC CATCTTAATG TATATCACAT CACTCTTCCA AGTTTTGCCT 
CAACAAGTGA GCATTGAAGC CATCCAGGAA GTGGAAATGT TGCCAAGGCC ATCAAAAGTT 
ACTAGAGAAG AACATTTTCA ATTACATCAT CAAATGCACT ATTCTCAACA GATCACAGTC 
AGTCTAGCAC AGGGCTATGA ACGAGCCCCT TCCTCTCCTA AGCCTCGGTT CAAGAGCTAT 
GCCTACACAC AGGCTGCTTA TGTCACCACT TCTGACCCCA CACGGAGCCC ACTTCCTTCA 
CAGCATTTGG AAACTCCTGA AGACAAGTCA TTTGGCCGGT CATTGACAGA GACCGAAGCA 
AACCTGGACA GTTATCAAAC AGCTTTGGAA GAAGTACTCT CGTGGCTTCT TTCAGCTGAG 
GATGCACTGC AAGCCCAAGG AGAGATTTCT AATGATGTCG AAGAAGTGAA AGAACAATTT 
CATACTCATG AGGGATATAT GATGGACTTG ACATCCCATC AGGGACGGGT CGGTAATGTT 
CTCCAACTGG GAAGTCAACT GATTGGAACA GGGAAATTAT CAGAAGATGA AGAAACCGAA 
GTGCAGGAAC AAATGAATCT CCTCAATTCA AGATGGGAAT GCCTCAGGGT AGCTAGCATG 
GAAAAACAAA GCAATTTACA TAAAGTTCTA ATGGATCTCC AGAATCAGCA ACTGAAAGAG 
TTAAATGACT GGCTAACCAA AACAGAAGAG AGAACAAGGA AAATGGAGAA GGAGCCCCTT 
GGACCTGATA TTGAAGACCT AAAACGCCAA GTACAACAAC ATAAGGTGCT TCAAGAAGAC 
TTAGAACAGG AACAAGTCAG GGTCAATTCC CTCACTCATA TGGTGGTGGT AGTCGATGAA 
TCTAGTGGAG ACCATGCAAC TGCTGCTTTG GAAGAACAAC TTAAGGTACT GGGAGATCGA 
TGGGCAAACA TCTGTAGGTG GACAGAAGAT CGCTGGGTTC TTTTACAAGA CATCCTCCTA 
AAATGGCAGC GTTTTACTGA AGAACAGTGC CTTTTCAGTG CATGGCTTTC GGAGAAGGAA 
GATGCAGTGA ACAAGATTCA CACAACTGGC TTTAAGGATC AAAGTGAAGT GTTATCAAAT 
CTTCAGAAAC TGGCTGTCTT AAAAACAGAT CTGGAAAAGA AGAAGCAAAC CATGGACAAA 
CTCTGCTCAC TCAACCAAGA CCTTCTTTCA GCGCTGAAAA ACACAGTGGT AGCCCACAAG 
ATGGAAGCAT GGCTGGACAA CTTTGCCCAG CGCTGGGATA ATTTAGTCCA GAAACTTGAA 
AAAAGTTCAG CACAGATTTC ACAGGCTGTC ACCACCACTC AGCCATCACT AACACAGACA 
ACTGTAATGG AAACAGTAAC TATGGTGACC ACGAGGGAAC ACATCTTGGT AAAGCATGCC 
CAAGAGGAAC TGCCACCACC ACCCCCTCAG AAGAAGAGGC AGATTATCGT GGATTCTGAA 
ATTAGGAAAA GGTTGGATGT CGATATAACT GAACTTCACA GTTGGATTAC TCGTTCAGAA 
GCTGTGTTGC AGAGTCCTGA ATTTGCAATC TATCGGAAGG AAGGCAACTT CTCAGACCTT 
AAAGAAAAAG TCAATGCCAT AGAGCGAGAA AAAGCCGAGA AGTTCAGAAA ACTGCAAGAT 
GCCAGCAGAT CAGCTCAGGC CCTGGTGGAA CAGATGGTGA ATGAGGGTGT TAATGCTGAC 
AGCATCAAAC AAGCCTCCGA ACAACTGAAC AGCCGGTGGA TAGAGTTCTG CCAATTGCTA 
AGCGAGAGAC TTAACTGGCT GGAGTATCAG AACAACATCA TCACTTTCTA TAATCAGCTA 
CAACAATTGG AGCAGATGAC AACTACTGCT GAAAACTGGT TGAAAACCCA GCCTACCACC 
ACATCAGAGC CAACAGCAAT TAAAAGCCAG TTAAAAATTT GTAAGGATGA AATCAACCGA 
CTGTCAGCTC TTCAGCCTCA AATCGAGCGA TTAAAAATTC AAAGCATAGC CCTGAAAGAG 
AAAGGACAAG GGCCAATGTT CCTGGATGCA GACTTTGTGG CCTTTACAAA TCATTTTAAC 
CAAGTCTTTG CTGATGTGCA GGCAAGAGAA AAAGAGCTAC AAACAATTTT TGACAGTTTG 
CCACCCATGC GCTATCAGGA GACTATGAGT ACCATCCTGA CATGGATCCA GCAGTCAGAA 
ACCAAACTCT CTATACCTCA GGTTACTGTC ACTGAATATG ACATCATGGA ACAGAGACTC 
GGAGAGCTAC AGGCTTTACA AAGCTCTCTG CAAGAGCAAC AAAATGGCCT AAACTATCTC 
AGCACCACTG TGAAAGAGAT GTCAAAGAAA GCACCACTGT CTGATATTAG TCGGAAATAT 
CAATCAGAAT TTGAAGAGAT TGAGGGACGT TGGAAGAAGC TGTCTTCCCA GCTGGTTGAA 
CATTGTCAAA AGTTGGAGGA GCAAATGGCT AAACTTCGAA AAATTCAGAA TCACATAAAA 
ACTCTGAAGA AATGGATCAC TGAAGTCGAT GTTTTCCTGA AGGAGGAATG GCCTGCCCTT 
GGGGATTCAG AAATTCTGAA AAGACAGCTG AAACAGTGCA GGCTTTTAGT CAATGACATT 
CAGACCATCC AGCCTAGTCT CAACAGTGTC AATGAAGGGG CTCAGAAGAT GAAGAATGAA 
GCAGAACCAG AGTTTGCTGG CAGACTTGAG ACAGAGCTCC GAGAACTTAA CACCCAGTGG 
GATTACATGT GCCGCCAGGT CTATGCCAGG AAGGAAGCCT TAAAAGGAGG TTTGGATAAA 
ACTGTAAGTC TTCAGAAAGA TCTGTCAGAG ATGCATGAGT GGATGACACA AGCTGAAGAA 
GAATACCTAG AGAGAGATTT CGAATACAAG ACCCCTGATG AATTACAGAC AGCAGTTGAA 
GAGATGAAGA GAGCTAAAGA AGAGGCCCAG CAAAAAGAAG CAAAAGTGAA ACTCCTAACC 
GAGTCCGTCA ATAGTGTCAT AGCTCAGGCT CCACCTGCAG CACAAGAGGC CTTAAAAAAG 
GAACTTGACA CTCTCACCAC CAACTACCAG TGGCTCTGCA CCAGGCTCAA TGGCAAATGC 
AAGACCTTGG AAGAAGTTTG GGCGTGCTGG CATGAGTTAT TGTCCTACTT GGAGAAGGCA 
AACAAGTGGC TAAGTGAAGT AGAAGTCAAG CTTAAAACCA CTGAAAATAT TTCTGGGGGA 
GCTGAGGAAA TCGCCGAGGT GCTTGATTCG CTTGAAAATT TGATGCAACA TTCAGAGGAT 
AACCCGAATC AGATTCGCAT ATTGGCACAG ACCTTGACAG ATGGTGGAGT CATGGATGAA 
CTGATCAATG AGGAGCTTGA GACATTTAAT TCTCGTTGGA GAGAACTCCA TGAAGAGGCT 
GTGAGGAGGC AAAAGTTGCT TGAGCAGAGT ATCCAGTCGG CCCAGGAGAT AGAAAAATCC 
TTGCACTTAA TTCAGGAGTC CCTCTCTTCC ATTGACAAGC AGTTGGCAGC TTATATTGCT 
GACAAAGTGG ATGCAGCTCA GATGCCTCAG GAAGCCCAGA AAATCCAATC AGATTTGACA 
AGTCATGAGA TCAGTTTAGA AGAAATGAAG AAACATAACC AGGGAAAGGA GACTGCCCAA 
AGGGTACTAT CCCAAATTGA TGTGGCACAG AAAAAATTGC AGGATGTTTC CATGAAGTTT 
CGATTATTCC AGAAGCCAGC CAATTTTGAG CAGCGCCTAC AAGAAAGTAA AATGATTTTA 
GATGAAGTGA AGATGCATTT ACCTGCGTTG GAAACAAAGA GTGTGGAACA GGAAGTAGTA 
CAGTCACAGT TAAATCATTG TGTGAACTTG TATAAAAGTC TGAGTGAAGT GAAGTCTGAA 
GTGGAAATGG TAATAAAAAC TGGACGTCAG ATTGTACAGA AGAAGCAGAC GGAAAACCCG 
AAAGAGCTTG ATGAAAGAGT TACAGCTTTG AAATTGCATT ATAATGAGCT GGGAGCAAAG 
GTGACAGAAA GAAAGCAACA GTTGGAAAAA TGCTTGAAAT TGTCCCGTAA GATGCGAAAG 
GAAATGAATG CCCTGACAGA ATGGCTGGCA GCGACAGATA TGGAACTGAC GAAGAGATCG 
GCAGTTGAAG GAATGCCTAG TAATTTGGAT TCTGAAGTTG CCTGGGGAAA GGCTACTCAG 
AAAGAGATTG AGAAACAGAA GGTTCACCTA AAGAGTGTCA CAGAGGTAGG AGAGGCCTTG 
AAAACGGTTT TGGGCAAGAA GGAAATGTTG GTGGAAGATA AACTGAGTCT TCTGAATAGT 
AACTGGATAG CCGTCACTTC CCGAGCAGAA GAGTGGTTAA ACCTTTTATT GGAATACCAG 
AAACACATGG AAAACTTTGA CCAGAATGTG GATTACATCA CAAACTGGAT CATTCAGGCT 
GATGCACTTT TGGATGAATC TGAGAAAAAG AAACCTCAGC AAAAAGAAGA CATACTTAAG 
CGTTTAAAGG CTGAAATGAA TGACATACGT CCAAAGGTGG ATTCTACACG TGACCAAGCA 
GCAAACCTGA TGGCAAACCG CGGCGACCAC TGCAGGAAAG TAGTAGAGCC CAAAATCTCA 
GAGCTCAACC ATCGATTTGC AGCCATTTCT CACAGAATTA AGACTGGAAA GGCCTCCATT 
CCTTTGAAGG AATTGGAGCA GTTTAACTCA GATATACAAA AATTGCTTGA ACCACTGGAG 
GCTGAAATTC AGCAGGGGGT GAATCTGAAA GAGGAAGACT TCAATAAAGA TATGAGTGAA 
GACAATGAGG GTACTGTAAA AGAATTGTTG CAAAGAGGAG ACAACTTACA ACAAAGAATC 
ACAGATGAGA GAAAGCGAGA GGAAATAAAG ATAAAACAAC AGCTGTTACA GACAAAACAT 
AATGCTCTCA AGGATTTGAG GTCTCAAAGA AGAAAAAAGG CTCTAGAAAT TTCTCACCAG 
TGGTATCAGT ACAAGAGGCA GGCTGATGAT CTCCTGAAAT GCTTGGATGA CATTGAAAAA 
AAATTAGCCA GCCTACCTGA ACCCAGAGAT GAAAGGAAAA TAAAGGAAAT TGATCGTGAA 
TTGCAGAAGA AGAAAGAGGA GCTGAATGCA GTGCGTAGGC AAGCTGAGGG CTTGTCTGAG 
GATGGGGCCG CAATGGCAGT GGAGCCAACT CAGATCCAGC TCAGCAAGCG CTGGCGGGAA 
ATTGAGAGCA AATTTGCTCA GTTTCGAAGA CTCAACTTTG CACAAATTCA CACTGTCCAT 
GAAGAGTCAG TGGTGGCGAT GACTGAAGAC ATGCCTTTGG AAATTTCTTA TGTGCCTTCT 
ACTTACCTGA CTGAGATCAC TCATGTCTCA CAAGCCCTAT CAGAAGTGGA AGAGCTTCTT 
AATGCTCCCG ACCTTTGTGC TCAAGATTTT GAAGATCTCT TTAAACAAGA GGAATCCTTG 
AAGAACATAA AAGACAGCCT GCAACAAATC TCAGGTCGGA TTGACATCAT TCACAATAAA 
AAGACAGCAG CATTGCACAG TGCCACTCCT GCAGAAAGGG CAAAGCTCCA GGAAGCTCTC 
TCACGGCTTG ATTTCCAATG GGAAAGAGTT AACAATATGT ACAAGGACCG ACAAGGGAGA 
TTTGACAGAT CTGTGGAAAA ATGGCGGCGG TTTCATTATG ATATGAAGAT ACTTAATCAA 
TGGCTAACAG AAGCTGAACA GTTTCTCAAA AAGACACAAA TTCCTGAGAA TTGGGAACAT 
GCCAAATACA AATGGTATCT TAAGGAACTC CAGGATGGCA TTGGACAGCG GCAAAGTGTT 
GTCAGGGTAT TGAATGCAAC TGGGGAAGAA ATAATTCAAC AGTCCTCAAA AACAGATGCC 
AGTATTCTCC AAGAAAAACT GGGAAGCCTG AATCTGCGGT GGCAGGAGGT CTGCAAACAG 
CTGGCAGAAA GAAAAAAGAG GCTAGAGGAA CAGAAGAATA TCTTGTCAGA ATTTCAAAGA 
GATGTAAATG AATTTGTTTT ATGGTTGGAA GAAGCGGATA ACGTTGCTAA TATTCCACTT 
GAACCTGGAA ATGAGCAGCA GCTAAAAGAA AAACTTGAAC AAGTCAAGTT ACTGGCAGAA 
GAGTTGCCCC TGCGCCAGGG AATTCTAAAA CAATTAAATG AAACTGGAGG AACAGTGCTT 
GTAAGTGCTC CCCTAAGCCC AGAAGAGCAA GATAAACTTG AAAATAAGCT CAAGCAGACA 
AATCTTCAGT GGATAAAGGT TTCTAGAAAT CTGCCTGAGA AGCAAGAAGA AATTGAGGCA 
CACGTAAAAG ACCTTGGACA GCTGGAAGAG CAGTTAAATC ATCTGCTTCT ATGGCTGTCT 
CCTATTAGGA ATCAGTTGGA AATTTACAAT CAGCCAAATC AAACAGGACC ATTTGACATC 
AAGGAAATTG AAGTAGCAGT TCAAGCTAAA CAGCCGGATG TGGAAGGGAT TTTGTCTAAA 
GGGCAGCATT TGTACAAGGA AAAACCAGCC ACTCAGCCAG CGAAGAGAAA GCTGGAAGAT 
CTCAGCTCTG ATTGGAAGGT GGTAACTCAG TTGCTTCAAG AGCTGCGGGC AAAGCAGCCT 
GGCCCAGCTC CTGGACTGAC CACTGTCAGA GCCCCTCCCA GTCAGACTGT TACTCTGGTG 
ACACAACCCG CGGTTACCAA GGAAACTGCC ATCTCCAAAC TAGAAATGCC ATCTTCATTG 
CTGTTGGAGG TACCTGCACT GGCAGATTTC AACCGAGCTT GGACAGAACT TACCGACTGG 
CTGTCTCTGC TTGATCGAGT TATAAAATCA CAGAGGGTGA TGGTGGGTGA TCTTGAAGAC 
ATTAACGAGA TGATCATCAA GCAGAAGGCA ACGCTGCAGG ATTTGGAACA GAGGCGCCCC 
CAGTTGGAAG AACTCATTAC CGCTGCCCAG AATTTGAAAA ACAAGACCAG CAATCAAGAG 
GCTAGAACAA TCATTACTGA TCGAATTGAA AGAATTCAGA GTCAGTGGGA TGAAGTACAG 
GAACATCTTC AGAACCGGAG GCAACAGTTG AATGAAATGT TAAAGGATTC CACACAATGG 
CTGGAAGCTA AAGAGGAGGC TGAGCAGGTG TTGGGGCAGG CCAGAGCCAA GCTTGAGTCA 
TGGAAGGAGG CTCCCTACAC AGTAGATGCA ATCCAAAAGA AAATCACAGA AACCAAGTTG 
GCCAAAGACC TCCGCCAGTG GCAGATAAAT GTAGATGTTG CAAATGATTT GGCACTGAAA 
CTTCTCCGAG ATTATTCTGC AGATGATACC AGAAAAGTAC ACATGATAAC AGAGAACATC 
AATGCCTCTT GGGCAAGCAT CCATAAAAGA TTGAGTGAGC GAGAGGCTGC TCTGGAAGAA 
ACCCACAGAT TACTGCAACA GTTCCCCTTG GACCTGGAGA AGTTCCTTGC CTGGCTTACA 
GAAGCCGAAA CAACTGCCAA CGTCCTGCAG GATGCCACCC ATAAGGAAAG GCTTCTAGAA 
GATTCCAAGG GAGTAAGAGA GCTGATGAAA CAATGGCAAG ATCTCCAAGG AGAAATCGAA 
GCTCACACAG ATATCTATCA CAACCTGGAC GAAAATGGCC AAAAAGTCCT GAGATCCCTG 
GAAGGTTCTG ACGATGCAGC CTTGTTGCAA AGACGTTTGG ATAACATGAA CTTCAAGTGG 
AGCGAACTTC GGAAAAAGTC TCTCAACATT AGGTCTCACT TGGAAGCCAG TTCTGACCAG 
TGGAAGCGTC TGCACCTTTC TCTTCAGGAA CTTCTGGTAT GGCTCCAGCT GAAAGATGAT 
GAGTTAAGCC GGCAGGCACC CATTGGAGGA GACTTTCCAG CGGTGCAGAA GCAGAATGAT 
GTACACAGGG CCTTCAAGAG GGAATTGAAA ACGAAAGAAC CTGTAATCAT GAGTACTCTT 
GAGACTGTAC GAATATTTCT GACAGAGCAG CCTTTAGAAG GACTAGAGAA ACTCTACCAG 
GAGCCCAGAG AGCTGCCTCC TGAAGAGAGA GCCCAGAATG TCACACGGCT CCTACGAAAG 
CAAGCTGAGG AGGTCAACAC TCAGTGGGAA AAACTGAACG TGCACTCTGC AGACTGGCAG 
AGAAAAATAG ACGAGGCCCT CGAAAGACTC CAGGAGCTTC AGGAAGCAAC AGATGAGCTG 
GATCTCAAAC TACGTCAGGC AGAGGTGATC AAAGGATCCT GGCAGCCTGT GGGTGATCTC 
CTCATTGACT CTCTCCAAGA TCACCTCGAA AAAGTCAAGG CGCTTCGAGG AGAAATTACA 
CCTCTGAAAG AGAATGTCAG CTACGTCAAT GACCTTGCTC GCCAACTCAC TACGTTGGGC 
ATTCAGCTGT CACCATATAA CCTCAACACT CTGGAAGACC TGAACACCAG ATGGAAGCTT 
CTGCAGGTGG CCATTGAGGA CCGCATCAGG CAGCTGCATG AAGCGCACAG GGACTTTGGA 
CCAGCCTCCC AGCACTTCCT TTCCACTTCT GTCCAGGGTC CCTGGGAGAG AGCCATCTCA 
CCAAACAAAG TGCCCTACTA TATCAACCAC GAGACCCAAA CAACTTGCTG GGACCATCCC 
AAAATGACAG AGCTCTACCA GTCTTTAGCT GACCTGAATA ATGTCAGGTT CTCAGCTTAC 
AGGACTGCCA TGAAACTCCG AAGACTGCAG AAGGCCCTTT GCTTGGATCT CTTGAGCCTA 
TCGGCTGCAT GCGATGCCTT GGACCAGCAC AACCTCAAGC AAAATGACCA GCCCATGGAT 
ATCCTGCAGG TCATTAACTG TCTGACCACT ATTTATGATC GCCTAGAGCA AGAGCACAAC 
AATCTGGTCA ACGTCCCTCT CTGCGTGGAT ATGTGTCTCA ATTGGCTGCT GAATGTTTAT 
GACACGGGAC GAACGGGGAG GATCCGGGTC CTGTCTTTTA AAACTGGCAT CATTTCTCTG 
TGTAAAGCCC ATTTGGAAGA CAAGTACAGA TACCTCTTCA AGCAAGTGGC AAGTTCGACA 
GGATTTTGTG ACCAGCGCAG GCTGGGCCTC CTCCTGCATG ACTCTATCCA GATCCCAAGA 
CAGTTGGGTG AAGTCGCATC GTTCGGGGGC AGTAACATTG AGCCGAGTGT CAGGAGCTGC 
TTCCAGTTTG CTAATAATAA GCCTGAGATC GAAGCGGCCC TCTTCCTAGA CTGGATGCGC 
CTGGAGCCCC AGTCCATGGT GTGGCTGCCT GTCCTGCACC GAGTGGCTGC CGCGGAAACT 
GCCAAGCACC AGGCCAAGTG CAACATCTGC AAGGAGTGTC CCATCATCGG ATTCAGGTAC 
AGGAGTCTAA AGCACTTTAA TTATGACATC TGCCAAAGTT GCTTTTTTTC TGGTCGAGTT 
GCAAAAGGCC ATAAAATGCA CTATCCCATG GTGGAATACT GCACTCCGAC TACATCGGGA 
GAAGATGTCC GTGACTTTGC CAAGGTACTA AAAAACAAAT TTCGAACCAA AAGGTATTTT 
GCGAAGCATC CCCGAATGGG CTACCTGCCA GTGCAGACTG TCTTAGAGGG GGACAACATG 
GAAACTCCTG TCACTCTGAT CAACTTCTGG CCGGTAGATT CTGCGCCTGC CTCGTCCCCT 
CAGCTTTCAC ACGATGATAC TCATTCACGC ATTGAGCATT ATGCTAGCAG GCTAGCAGAA 
ATGGAAAACA GCAATGGATC TTATCTAAAT GATAGCATCT CTCCTAATGA GAGCATAGAT 
GATGAACATT TGTTAATCCA GCATTACTGC CAAAGTTTGA ACCAGGACTC CCCCCTGAGC 
CAGCCTCGTA GTCCTGCCCA GATCTTGATT TCCTTAGAGA GTGAGGAAAG AGGGGAGCTA 
GAGAGAATCC TAGCAGATCT TGAGGAAGAA AACAGAAATC TGCAAGCAGA GTATGATCGT 
CTAAAGCAGC AGCATGAACA CAAAGGCCTG TCCCCACTGC CATCCCCTCC TGAAATGATG 
CCTACTTCTC CCCAAAGTCC CCGGGATGCT GAGCTCATCG CTGAGGCCAA GCTGCTGCGT 
CAACACAAAG GCCGCCTGGA AGCCAGGATG CAAATCCTTG AAGACCATAA CAAACAACTG 
GAATCCCAGT TACACAGGCT CAGGCAGCTG CTGGAGCAAC CCCAGGCAGA GGCCAAGGTG 
AATGGTACAA CGGTGTCTTC TCCTTCTACC TCTCTTCAGA GGTCAGATAG CAGTCAGCCT 
ATGCTGCTCC GGGTGGTCGG CAGTCAGACT TCAGAATCCA TGGGCGAGGA AGACCTGCTC 
AGCCCTCCCC AGGACACAAG CACAGGGTTA GAGGAAGTGA TGGAGCAGCT CAACCACTCC 
TTCCCTAGTT CCAGAGGAAG AAATACCCCT GGGAAGCCAA TGAGAGAGGA CACAATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMD ( XM_014111346.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013966821.1 Canis lupus familiaris dystrophin (DMD), transcript variant X7, mRNA.

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CloneID	OCe45417
Clone ID Related Accession (Same CDS sequence)	XM_014111346.1 , XM_014111346.2
Accession Version	XM_014111346.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11031bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	dystrophin isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTGGT GGGAAGAAGT AGAGGACTGT TATGAAAGAG AAGATGTTCA AAAGAAAACA 
TTCACAAAAT GGATAAATGC ACAGTTTTCT AAGTTTGGGA AGCAGCACAT AGAGAACCTC 
TTCAGTGACC TACAGGATGG GAGACGCCTC CTAGACCTTT TGGAAGGCCT GACAGGGCAA 
AAACTGCCAA AAGAAAAAGG ATCCACAAGA GTTCATGCCC TGAACAATGT CAACAAGGCA 
CTGCGCGTCT TGCAGAAAAA TAATGTTGAT TTAGTGAACA TTGGAAGTAC TGACATAGTA 
GATGGAAATC ACAAACTGAC TCTTGGTTTG ATTTGGAATA TAATCCTCCA CTGGCAGGTC 
AAAAATGTAA TGAAAAATAT CATGGCTGGA TTGCAACAAA CCAACAGTGA AAAGATTCTC 
CTGAGCTGGG TCCGACAATC AACTCGTAAT TATCCACAGG TTAATGTCAT TAACTTCACC 
ACCAGCTGGT CTGATGGCCT GGCTTTGAAC GCTCTCATCC ACAGTCATAG GCCAGACCTG 
TTTGATTGGA ATAGTGTGGT TTGCCAGCAG TCAGCCACAC AACGCCTGGA ACATGCATTC 
AACATTGCCA AATATCAATT AGGCATAGAG AAACTGCTTG ATCCTGAAGA TGTTGCCACC 
ACTTATCCAG ATAAGAAGTC CATCTTAATG TATATCACAT CACTCTTCCA AGTTTTGCCT 
CAACAAGTGA GCATTGAAGC CATCCAGGAA GTGGAAATGT TGCCAAGGCC ATCAAAAGTT 
ACTAGAGAAG AACATTTTCA ATTACATCAT CAAATGCACT ATTCTCAACA GATCACAGTC 
AGTCTAGCAC AGGGCTATGA ACGAGCCCCT TCCTCTCCTA AGCCTCGGTT CAAGAGCTAT 
GCCTACACAC AGGCTGCTTA TGTCACCACT TCTGACCCCA CACGGAGCCC ACTTCCTTCA 
CAGCATTTGG AAACTCCTGA AGACAAGTCA TTTGGCCGGT CATTGACAGA GACCGAAGCA 
AACCTGGACA GTTATCAAAC AGCTTTGGAA GAAGTACTCT CGTGGCTTCT TTCAGCTGAG 
GATGCACTGC AAGCCCAAGG AGAGATTTCT AATGATGTCG AAGAAGTGAA AGAACAATTT 
CATACTCATG AGGGATATAT GATGGACTTG ACATCCCATC AGGGACGGGT CGGTAATGTT 
CTCCAACTGG GAAGTCAACT GATTGGAACA GGGAAATTAT CAGAAGATGA AGAAACCGAA 
GTGCAGGAAC AAATGAATCT CCTCAATTCA AGATGGGAAT GCCTCAGGGT AGCTAGCATG 
GAAAAACAAA GCAATTTACA TAAAGTTCTA ATGGATCTCC AGAATCAGCA ACTGAAAGAG 
TTAAATGACT GGCTAACCAA AACAGAAGAG AGAACAAGGA AAATGGAGAA GGAGCCCCTT 
GGACCTGATA TTGAAGACCT AAAACGCCAA GTACAACAAC ATAAGGTGCT TCAAGAAGAC 
TTAGAACAGG AACAAGTCAG GGTCAATTCC CTCACTCATA TGGTGGTGGT AGTCGATGAA 
TCTAGTGGAG ACCATGCAAC TGCTGCTTTG GAAGAACAAC TTAAGGTACT GGGAGATCGA 
TGGGCAAACA TCTGTAGGTG GACAGAAGAT CGCTGGGTTC TTTTACAAGA CATCCTCCTA 
AAATGGCAGC GTTTTACTGA AGAACAGTGC CTTTTCAGTG CATGGCTTTC GGAGAAGGAA 
GATGCAGTGA ACAAGATTCA CACAACTGGC TTTAAGGATC AAAGTGAAGT GTTATCAAAT 
CTTCAGAAAC TGGCTGTCTT AAAAACAGAT CTGGAAAAGA AGAAGCAAAC CATGGACAAA 
CTCTGCTCAC TCAACCAAGA CCTTCTTTCA GCGCTGAAAA ACACAGTGGT AGCCCACAAG 
ATGGAAGCAT GGCTGGACAA CTTTGCCCAG CGCTGGGATA ATTTAGTCCA GAAACTTGAA 
AAAAGTTCAG CACAGATTTC ACAGGCTGTC ACCACCACTC AGCCATCACT AACACAGACA 
ACTGTAATGG AAACAGTAAC TATGGTGACC ACGAGGGAAC ACATCTTGGT AAAGCATGCC 
CAAGAGGAAC TGCCACCACC ACCCCCTCAG AAGAAGAGGC AGATTATCGT GGATTCTGAA 
ATTAGGAAAA GGTTGGATGT CGATATAACT GAACTTCACA GTTGGATTAC TCGTTCAGAA 
GCTGTGTTGC AGAGTCCTGA ATTTGCAATC TATCGGAAGG AAGGCAACTT CTCAGACCTT 
AAAGAAAAAG TCAATGCCAT AGAGCGAGAA AAAGCCGAGA AGTTCAGAAA ACTGCAAGAT 
GCCAGCAGAT CAGCTCAGGC CCTGGTGGAA CAGATGGTGA ATGAGGGTGT TAATGCTGAC 
AGCATCAAAC AAGCCTCCGA ACAACTGAAC AGCCGGTGGA TAGAGTTCTG CCAATTGCTA 
AGCGAGAGAC TTAACTGGCT GGAGTATCAG AACAACATCA TCACTTTCTA TAATCAGCTA 
CAACAATTGG AGCAGATGAC AACTACTGCT GAAAACTGGT TGAAAACCCA GCCTACCACC 
ACATCAGAGC CAACAGCAAT TAAAAGCCAG TTAAAAATTT GTAAGGATGA AATCAACCGA 
CTGTCAGCTC TTCAGCCTCA AATCGAGCGA TTAAAAATTC AAAGCATAGC CCTGAAAGAG 
AAAGGACAAG GGCCAATGTT CCTGGATGCA GACTTTGTGG CCTTTACAAA TCATTTTAAC 
CAAGTCTTTG CTGATGTGCA GGCAAGAGAA AAAGAGCTAC AAACAATTTT TGACAGTTTG 
CCACCCATGC GCTATCAGGA GACTATGAGT ACCATCCTGA CATGGATCCA GCAGTCAGAA 
ACCAAACTCT CTATACCTCA GGTTACTGTC ACTGAATATG ACATCATGGA ACAGAGACTC 
GGAGAGCTAC AGGCTTTACA AAGCTCTCTG CAAGAGCAAC AAAATGGCCT AAACTATCTC 
AGCACCACTG TGAAAGAGAT GTCAAAGAAA GCACCACTGT CTGATATTAG TCGGAAATAT 
CAATCAGAAT TTGAAGAGAT TGAGGGACGT TGGAAGAAGC TGTCTTCCCA GCTGGTTGAA 
CATTGTCAAA AGTTGGAGGA GCAAATGGCT AAACTTCGAA AAATTCAGAA TCACATAAAA 
ACTCTGAAGA AATGGATCAC TGAAGTCGAT GTTTTCCTGA AGGAGGAATG GCCTGCCCTT 
GGGGATTCAG AAATTCTGAA AAGACAGCTG AAACAGTGCA GGCTTTTAGT CAATGACATT 
CAGACCATCC AGCCTAGTCT CAACAGTGTC AATGAAGGGG CTCAGAAGAT GAAGAATGAA 
GCAGAACCAG AGTTTGCTGG CAGACTTGAG ACAGAGCTCC GAGAACTTAA CACCCAGTGG 
GATTACATGT GCCGCCAGGT CTATGCCAGG AAGGAAGCCT TAAAAGGAGG TTTGGATAAA 
ACTGTAAGTC TTCAGAAAGA TCTGTCAGAG ATGCATGAGT GGATGACACA AGCTGAAGAA 
GAATACCTAG AGAGAGATTT CGAATACAAG ACCCCTGATG AATTACAGAC AGCAGTTGAA 
GAGATGAAGA GAGCTAAAGA AGAGGCCCAG CAAAAAGAAG CAAAAGTGAA ACTCCTAACC 
GAGTCCGTCA ATAGTGTCAT AGCTCAGGCT CCACCTGCAG CACAAGAGGC CTTAAAAAAG 
GAACTTGACA CTCTCACCAC CAACTACCAG TGGCTCTGCA CCAGGCTCAA TGGCAAATGC 
AAGACCTTGG AAGAAGTTTG GGCGTGCTGG CATGAGTTAT TGTCCTACTT GGAGAAGGCA 
AACAAGTGGC TAAGTGAAGT AGAAGTCAAG CTTAAAACCA CTGAAAATAT TTCTGGGGGA 
GCTGAGGAAA TCGCCGAGGT GCTTGATTCG CTTGAAAATT TGATGCAACA TTCAGAGGAT 
AACCCGAATC AGATTCGCAT ATTGGCACAG ACCTTGACAG ATGGTGGAGT CATGGATGAA 
CTGATCAATG AGGAGCTTGA GACATTTAAT TCTCGTTGGA GAGAACTCCA TGAAGAGGCT 
GTGAGGAGGC AAAAGTTGCT TGAGCAGAGT ATCCAGTCGG CCCAGGAGAT AGAAAAATCC 
TTGCACTTAA TTCAGGAGTC CCTCTCTTCC ATTGACAAGC AGTTGGCAGC TTATATTGCT 
GACAAAGTGG ATGCAGCTCA GATGCCTCAG GAAGCCCAGA AAATCCAATC AGATTTGACA 
AGTCATGAGA TCAGTTTAGA AGAAATGAAG AAACATAACC AGGGAAAGGA GACTGCCCAA 
AGGGTACTAT CCCAAATTGA TGTGGCACAG AAAAAATTGC AGGATGTTTC CATGAAGTTT 
CGATTATTCC AGAAGCCAGC CAATTTTGAG CAGCGCCTAC AAGAAAGTAA AATGATTTTA 
GATGAAGTGA AGATGCATTT ACCTGCGTTG GAAACAAAGA GTGTGGAACA GGAAGTAGTA 
CAGTCACAGT TAAATCATTG TGTGAACTTG TATAAAAGTC TGAGTGAAGT GAAGTCTGAA 
GTGGAAATGG TAATAAAAAC TGGACGTCAG ATTGTACAGA AGAAGCAGAC GGAAAACCCG 
AAAGAGCTTG ATGAAAGAGT TACAGCTTTG AAATTGCATT ATAATGAGCT GGGAGCAAAG 
GTGACAGAAA GAAAGCAACA GTTGGAAAAA TGCTTGAAAT TGTCCCGTAA GATGCGAAAG 
GAAATGAATG CCCTGACAGA ATGGCTGGCA GCGACAGATA TGGAACTGAC GAAGAGATCG 
GCAGTTGAAG GAATGCCTAG TAATTTGGAT TCTGAAGTTG CCTGGGGAAA GGCTACTCAG 
AAAGAGATTG AGAAACAGAA GGTTCACCTA AAGAGTGTCA CAGAGGTAGG AGAGGCCTTG 
AAAACGGTTT TGGGCAAGAA GGAAATGTTG GTGGAAGATA AACTGAGTCT TCTGAATAGT 
AACTGGATAG CCGTCACTTC CCGAGCAGAA GAGTGGTTAA ACCTTTTATT GGAATACCAG 
AAACACATGG AAAACTTTGA CCAGAATGTG GATTACATCA CAAACTGGAT CATTCAGGCT 
GATGCACTTT TGGATGAATC TGAGAAAAAG AAACCTCAGC AAAAAGAAGA CATACTTAAG 
CGTTTAAAGG CTGAAATGAA TGACATACGT CCAAAGGTGG ATTCTACACG TGACCAAGCA 
GCAAACCTGA TGGCAAACCG CGGCGACCAC TGCAGGAAAG TAGTAGAGCC CAAAATCTCA 
GAGCTCAACC ATCGATTTGC AGCCATTTCT CACAGAATTA AGACTGGAAA GGCCTCCATT 
CCTTTGAAGG AATTGGAGCA GTTTAACTCA GATATACAAA AATTGCTTGA ACCACTGGAG 
GCTGAAATTC AGCAGGGGGT GAATCTGAAA GAGGAAGACT TCAATAAAGA TATGAGTGAA 
GACAATGAGG GTACTGTAAA AGAATTGTTG CAAAGAGGAG ACAACTTACA ACAAAGAATC 
ACAGATGAGA GAAAGCGAGA GGAAATAAAG ATAAAACAAC AGCTGTTACA GACAAAACAT 
AATGCTCTCA AGGATTTGAG GTCTCAAAGA AGAAAAAAGG CTCTAGAAAT TTCTCACCAG 
TGGTATCAGT ACAAGAGGCA GGCTGATGAT CTCCTGAAAT GCTTGGATGA CATTGAAAAA 
AAATTAGCCA GCCTACCTGA ACCCAGAGAT GAAAGGAAAA TAAAGGAAAT TGATCGTGAA 
TTGCAGAAGA AGAAAGAGGA GCTGAATGCA GTGCGTAGGC AAGCTGAGGG CTTGTCTGAG 
GATGGGGCCG CAATGGCAGT GGAGCCAACT CAGATCCAGC TCAGCAAGCG CTGGCGGGAA 
ATTGAGAGCA AATTTGCTCA GTTTCGAAGA CTCAACTTTG CACAAATTCA CACTGTCCAT 
GAAGAGTCAG TGGTGGCGAT GACTGAAGAC ATGCCTTTGG AAATTTCTTA TGTGCCTTCT 
ACTTACCTGA CTGAGATCAC TCATGTCTCA CAAGCCCTAT CAGAAGTGGA AGAGCTTCTT 
AATGCTCCCG ACCTTTGTGC TCAAGATTTT GAAGATCTCT TTAAACAAGA GGAATCCTTG 
AAGAACATAA AAGACAGCCT GCAACAAATC TCAGGTCGGA TTGACATCAT TCACAATAAA 
AAGACAGCAG CATTGCACAG TGCCACTCCT GCAGAAAGGG CAAAGCTCCA GGAAGCTCTC 
TCACGGCTTG ATTTCCAATG GGAAAGAGTT AACAATATGT ACAAGGACCG ACAAGGGAGA 
TTTGACAGAT CTGTGGAAAA ATGGCGGCGG TTTCATTATG ATATGAAGAT ACTTAATCAA 
TGGCTAACAG AAGCTGAACA GTTTCTCAAA AAGACACAAA TTCCTGAGAA TTGGGAACAT 
GCCAAATACA AATGGTATCT TAAGGAACTC CAGGATGGCA TTGGACAGCG GCAAAGTGTT 
GTCAGGGTAT TGAATGCAAC TGGGGAAGAA ATAATTCAAC AGTCCTCAAA AACAGATGCC 
AGTATTCTCC AAGAAAAACT GGGAAGCCTG AATCTGCGGT GGCAGGAGGT CTGCAAACAG 
CTGGCAGAAA GAAAAAAGAG GCTAGAGGAA CAGAAGAATA TCTTGTCAGA ATTTCAAAGA 
GATGTAAATG AATTTGTTTT ATGGTTGGAA GAAGCGGATA ACGTTGCTAA TATTCCACTT 
GAACCTGGAA ATGAGCAGCA GCTAAAAGAA AAACTTGAAC AAGTCAAGTT ACTGGCAGAA 
GAGTTGCCCC TGCGCCAGGG AATTCTAAAA CAATTAAATG AAACTGGAGG AACAGTGCTT 
GTAAGTGCTC CCCTAAGCCC AGAAGAGCAA GATAAACTTG AAAATAAGCT CAAGCAGACA 
AATCTTCAGT GGATAAAGGT TTCTAGAAAT CTGCCTGAGA AGCAAGAAGA AATTGAGGCA 
CACGTAAAAG ACCTTGGACA GCTGGAAGAG CAGTTAAATC ATCTGCTTCT ATGGCTGTCT 
CCTATTAGGA ATCAGTTGGA AATTTACAAT CAGCCAAATC AAACAGGACC ATTTGACATC 
AAGGAAATTG AAGTAGCAGT TCAAGCTAAA CAGCCGGATG TGGAAGGGAT TTTGTCTAAA 
GGGCAGCATT TGTACAAGGA AAAACCAGCC ACTCAGCCAG CGAAGAGAAA GCTGGAAGAT 
CTCAGCTCTG ATTGGAAGGT GGTAACTCAG TTGCTTCAAG AGCTGCGGGC AAAGCAGCCT 
GGCCCAGCTC CTGGACTGAC CACTGTCAGA GCCCCTCCCA GTCAGACTGT TACTCTGGTG 
ACACAACCCG CGGTTACCAA GGAAACTGCC ATCTCCAAAC TAGAAATGCC ATCTTCATTG 
CTGTTGGAGG TACCTGCACT GGCAGATTTC AACCGAGCTT GGACAGAACT TACCGACTGG 
CTGTCTCTGC TTGATCGAGT TATAAAATCA CAGAGGGTGA TGGTGGGTGA TCTTGAAGAC 
ATTAACGAGA TGATCATCAA GCAGAAGGCA ACGCTGCAGG ATTTGGAACA GAGGCGCCCC 
CAGTTGGAAG AACTCATTAC CGCTGCCCAG AATTTGAAAA ACAAGACCAG CAATCAAGAG 
GCTAGAACAA TCATTACTGA TCGAATTGAA AGAATTCAGA GTCAGTGGGA TGAAGTACAG 
GAACATCTTC AGAACCGGAG GCAACAGTTG AATGAAATGT TAAAGGATTC CACACAATGG 
CTGGAAGCTA AAGAGGAGGC TGAGCAGGTG TTGGGGCAGG CCAGAGCCAA GCTTGAGTCA 
TGGAAGGAGG CTCCCTACAC AGTAGATGCA ATCCAAAAGA AAATCACAGA AACCAAGCAG 
TTGGCCAAAG ACCTCCGCCA GTGGCAGATA AATGTAGATG TTGCAAATGA TTTGGCACTG 
AAACTTCTCC GAGATTATTC TGCAGATGAT ACCAGAAAAG TACACATGAT AACAGAGAAC 
ATCAATGCCT CTTGGGCAAG CATCCATAAA AGATTGAGTG AGCGAGAGGC TGCTCTGGAA 
GAAACCCACA GATTACTGCA ACAGTTCCCC TTGGACCTGG AGAAGTTCCT TGCCTGGCTT 
ACAGAAGCCG AAACAACTGC CAACGTCCTG CAGGATGCCA CCCATAAGGA AAGGCTTCTA 
GAAGATTCCA AGGGAGTAAG AGAGCTGATG AAACAATGGC AAGATCTCCA AGGAGAAATC 
GAAGCTCACA CAGATATCTA TCACAACCTG GACGAAAATG GCCAAAAAGT CCTGAGATCC 
CTGGAAGGTT CTGACGATGC AGCCTTGTTG CAAAGACGTT TGGATAACAT GAACTTCAAG 
TGGAGCGAAC TTCGGAAAAA GTCTCTCAAC ATTAGGTCTC ACTTGGAAGC CAGTTCTGAC 
CAGTGGAAGC GTCTGCACCT TTCTCTTCAG GAACTTCTGG TATGGCTCCA GCTGAAAGAT 
GATGAGTTAA GCCGGCAGGC ACCCATTGGA GGAGACTTTC CAGCGGTGCA GAAGCAGAAT 
GATGTACACA GGGCCTTCAA GAGGGAATTG AAAACGAAAG AACCTGTAAT CATGAGTACT 
CTTGAGACTG TACGAATATT TCTGACAGAG CAGCCTTTAG AAGGACTAGA GAAACTCTAC 
CAGGAGCCCA GAGAGCTGCC TCCTGAAGAG AGAGCCCAGA ATGTCACACG GCTCCTACGA 
AAGCAAGCTG AGGAGGTCAA CACTCAGTGG GAAAAACTGA ACGTGCACTC TGCAGACTGG 
CAGAGAAAAA TAGACGAGGC CCTCGAAAGA CTCCAGGAGC TTCAGGAAGC AACAGATGAG 
CTGGATCTCA AACTACGTCA GGCAGAGGTG ATCAAAGGAT CCTGGCAGCC TGTGGGTGAT 
CTCCTCATTG ACTCTCTCCA AGATCACCTC GAAAAAGTCA AGGCGCTTCG AGGAGAAATT 
ACACCTCTGA AAGAGAATGT CAGCTACGTC AATGACCTTG CTCGCCAACT CACTACGTTG 
GGCATTCAGC TGTCACCATA TAACCTCAAC ACTCTGGAAG ACCTGAACAC CAGATGGAAG 
CTTCTGCAGG TGGCCATTGA GGACCGCATC AGGCAGCTGC ATGAAGCGCA CAGGGACTTT 
GGACCAGCCT CCCAGCACTT CCTTTCCACT TCTGTCCAGG GTCCCTGGGA GAGAGCCATC 
TCACCAAACA AAGTGCCCTA CTATATCAAC CACGAGACCC AAACAACTTG CTGGGACCAT 
CCCAAAATGA CAGAGCTCTA CCAGTCTTTA GCTGACCTGA ATAATGTCAG GTTCTCAGCT 
TACAGGACTG CCATGAAACT CCGAAGACTG CAGAAGGCCC TTTGCTTGGA TCTCTTGAGC 
CTATCGGCTG CATGCGATGC CTTGGACCAG CACAACCTCA AGCAAAATGA CCAGCCCATG 
GATATCCTGC AGGTCATTAA CTGTCTGACC ACTATTTATG ATCGCCTAGA GCAAGAGCAC 
AACAATCTGG TCAACGTCCC TCTCTGCGTG GATATGTGTC TCAATTGGCT GCTGAATGTT 
TATGACACGG GACGAACGGG GAGGATCCGG GTCCTGTCTT TTAAAACTGG CATCATTTCT 
CTGTGTAAAG CCCATTTGGA AGACAAGTAC AGATACCTCT TCAAGCAAGT GGCAAGTTCG 
ACAGGATTTT GTGACCAGCG CAGGCTGGGC CTCCTCCTGC ATGACTCTAT CCAGATCCCA 
AGACAGTTGG GTGAAGTCGC ATCGTTCGGG GGCAGTAACA TTGAGCCGAG TGTCAGGAGC 
TGCTTCCAGT TTGCTAATAA TAAGCCTGAG ATCGAAGCGG CCCTCTTCCT AGACTGGATG 
CGCCTGGAGC CCCAGTCCAT GGTGTGGCTG CCTGTCCTGC ACCGAGTGGC TGCCGCGGAA 
ACTGCCAAGC ACCAGGCCAA GTGCAACATC TGCAAGGAGT GTCCCATCAT CGGATTCAGG 
TACAGGAGTC TAAAGCACTT TAATTATGAC ATCTGCCAAA GTTGCTTTTT TTCTGGTCGA 
GTTGCAAAAG GCCATAAAAT GCACTATCCC ATGGTGGAAT ACTGCACTCC GACTACATCG 
GGAGAAGATG TCCGTGACTT TGCCAAGGTA CTAAAAAACA AATTTCGAAC CAAAAGGTAT 
TTTGCGAAGC ATCCCCGAAT GGGCTACCTG CCAGTGCAGA CTGTCTTAGA GGGGGACAAC 
ATGGAAACTC CTGTCACTCT GATCAACTTC TGGCCGGTAG ATTCTGCGCT AGCAGAAATG 
GAAAACAGCA ATGGATCTTA TCTAAATGAT AGCATCTCTC CTAATGAGAG CATAGATGAT 
GAACATTTGT TAATCCAGCA TTACTGCCAA AGTTTGAACC AGGACTCCCC CCTGAGCCAG 
CCTCGTAGTC CTGCCCAGAT CTTGATTTCC TTAGAGAGTG AGGAAAGAGG GGAGCTAGAG 
AGAATCCTAG CAGATCTTGA GGAAGAAAAC AGAAATCTGC AAGCAGAGTA TGATCGTCTA 
AAGCAGCAGC ATGAACACAA AGGCCTGTCC CCACTGCCAT CCCCTCCTGA AATGATGCCT 
ACTTCTCCCC AAAGTCCCCG GGATGCTGAG CTCATCGCTG AGGCCAAGCT GCTGCGTCAA 
CACAAAGGCC GCCTGGAAGC CAGGATGCAA ATCCTTGAAG ACCATAACAA ACAACTGGAA 
TCCCAGTTAC ACAGGCTCAG GCAGCTGCTG GAGCAACCCC AGGCAGAGGC CAAGGTGAAT 
GGTACAACGG TGTCTTCTCC TTCTACCTCT CTTCAGAGGT CAGATAGCAG TCAGCCTATG 
CTGCTCCGGG TGGTCGGCAG TCAGACTTCA GAATCCATGG GCGAGGAAGA CCTGCTCAGC 
CCTCCCCAGG ACACAAGCAC AGGGTTAGAG GAAGTGATGG AGCAGCTCAA CCACTCCTTC 
CCTAGTTCCA GAGGACACAA TGTAGGAAGC CTTTTCCACT TGGCAGATGA TTTGGGCAGA 
GCGATGGAGT CCTTAGTTTC AGTCATGACA GATGAAGAAG GGGCAGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013966821.1
CDS	248..11278
Translation	MLWWEEVEDCYEREDVQKKTFTKWINAQFSKFGKQHIENLFSDLQDGRRLLDLLEGLTGQKLPKEKGSTRVHALNNVNKALRVLQKNNVDLVNIGSTDIVDGNHKLTLGLIWNIILHWQVKNVMKNIMAGLQQTNSEKILLSWVRQSTRNYPQVNVINFTTSWSDGLALNALIHSHRPDLFDWNSVVCQQSATQRLEHAFNIAKYQLGIEKLLDPEDVATTYPDKKSILMYITSLFQVLPQQVSIEAIQEVEMLPRPSKVTREEHFQLHHQMHYSQQITVSLAQGYERAPSSPKPRFKSYAYTQAAYVTTSDPTRSPLPSQHLETPEDKSFGRSLTETEANLDSYQTALEEVLSWLLSAEDALQAQGEISNDVEEVKEQFHTHEGYMMDLTSHQGRVGNVLQLGSQLIGTGKLSEDEETEVQEQMNLLNSRWECLRVASMEKQSNLHKVLMDLQNQQLKELNDWLTKTEERTRKMEKEPLGPDIEDLKRQVQQHKVLQEDLEQEQVRVNSLTHMVVVVDESSGDHATAALEEQLKVLGDRWANICRWTEDRWVLLQDILLKWQRFTEEQCLFSAWLSEKEDAVNKIHTTGFKDQSEVLSNLQKLAVLKTDLEKKKQTMDKLCSLNQDLLSALKNTVVAHKMEAWLDNFAQRWDNLVQKLEKSSAQISQAVTTTQPSLTQTTVMETVTMVTTREHILVKHAQEELPPPPPQKKRQIIVDSEIRKRLDVDITELHSWITRSEAVLQSPEFAIYRKEGNFSDLKEKVNAIEREKAEKFRKLQDASRSAQALVEQMVNEGVNADSIKQASEQLNSRWIEFCQLLSERLNWLEYQNNIITFYNQLQQLEQMTTTAENWLKTQPTTTSEPTAIKSQLKICKDEINRLSALQPQIERLKIQSIALKEKGQGPMFLDADFVAFTNHFNQVFADVQAREKELQTIFDSLPPMRYQETMSTILTWIQQSETKLSIPQVTVTEYDIMEQRLGELQALQSSLQEQQNGLNYLSTTVKEMSKKAPLSDISRKYQSEFEEIEGRWKKLSSQLVEHCQKLEEQMAKLRKIQNHIKTLKKWITEVDVFLKEEWPALGDSEILKRQLKQCRLLVNDIQTIQPSLNSVNEGAQKMKNEAEPEFAGRLETELRELNTQWDYMCRQVYARKEALKGGLDKTVSLQKDLSEMHEWMTQAEEEYLERDFEYKTPDELQTAVEEMKRAKEEAQQKEAKVKLLTESVNSVIAQAPPAAQEALKKELDTLTTNYQWLCTRLNGKCKTLEEVWACWHELLSYLEKANKWLSEVEVKLKTTENISGGAEEIAEVLDSLENLMQHSEDNPNQIRILAQTLTDGGVMDELINEELETFNSRWRELHEEAVRRQKLLEQSIQSAQEIEKSLHLIQESLSSIDKQLAAYIADKVDAAQMPQEAQKIQSDLTSHEISLEEMKKHNQGKETAQRVLSQIDVAQKKLQDVSMKFRLFQKPANFEQRLQESKMILDEVKMHLPALETKSVEQEVVQSQLNHCVNLYKSLSEVKSEVEMVIKTGRQIVQKKQTENPKELDERVTALKLHYNELGAKVTERKQQLEKCLKLSRKMRKEMNALTEWLAATDMELTKRSAVEGMPSNLDSEVAWGKATQKEIEKQKVHLKSVTEVGEALKTVLGKKEMLVEDKLSLLNSNWIAVTSRAEEWLNLLLEYQKHMENFDQNVDYITNWIIQADALLDESEKKKPQQKEDILKRLKAEMNDIRPKVDSTRDQAANLMANRGDHCRKVVEPKISELNHRFAAISHRIKTGKASIPLKELEQFNSDIQKLLEPLEAEIQQGVNLKEEDFNKDMSEDNEGTVKELLQRGDNLQQRITDERKREEIKIKQQLLQTKHNALKDLRSQRRKKALEISHQWYQYKRQADDLLKCLDDIEKKLASLPEPRDERKIKEIDRELQKKKEELNAVRRQAEGLSEDGAAMAVEPTQIQLSKRWREIESKFAQFRRLNFAQIHTVHEESVVAMTEDMPLEISYVPSTYLTEITHVSQALSEVEELLNAPDLCAQDFEDLFKQEESLKNIKDSLQQISGRIDIIHNKKTAALHSATPAERAKLQEALSRLDFQWERVNNMYKDRQGRFDRSVEKWRRFHYDMKILNQWLTEAEQFLKKTQIPENWEHAKYKWYLKELQDGIGQRQSVVRVLNATGEEIIQQSSKTDASILQEKLGSLNLRWQEVCKQLAERKKRLEEQKNILSEFQRDVNEFVLWLEEADNVANIPLEPGNEQQLKEKLEQVKLLAEELPLRQGILKQLNETGGTVLVSAPLSPEEQDKLENKLKQTNLQWIKVSRNLPEKQEEIEAHVKDLGQLEEQLNHLLLWLSPIRNQLEIYNQPNQTGPFDIKEIEVAVQAKQPDVEGILSKGQHLYKEKPATQPAKRKLEDLSSDWKVVTQLLQELRAKQPGPAPGLTTVRAPPSQTVTLVTQPAVTKETAISKLEMPSSLLLEVPALADFNRAWTELTDWLSLLDRVIKSQRVMVGDLEDINEMIIKQKATLQDLEQRRPQLEELITAAQNLKNKTSNQEARTIITDRIERIQSQWDEVQEHLQNRRQQLNEMLKDSTQWLEAKEEAEQVLGQARAKLESWKEAPYTVDAIQKKITETKQLAKDLRQWQINVDVANDLALKLLRDYSADDTRKVHMITENINASWASIHKRLSEREAALEETHRLLQQFPLDLEKFLAWLTEAETTANVLQDATHKERLLEDSKGVRELMKQWQDLQGEIEAHTDIYHNLDENGQKVLRSLEGSDDAALLQRRLDNMNFKWSELRKKSLNIRSHLEASSDQWKRLHLSLQELLVWLQLKDDELSRQAPIGGDFPAVQKQNDVHRAFKRELKTKEPVIMSTLETVRIFLTEQPLEGLEKLYQEPRELPPEERAQNVTRLLRKQAEEVNTQWEKLNVHSADWQRKIDEALERLQELQEATDELDLKLRQAEVIKGSWQPVGDLLIDSLQDHLEKVKALRGEITPLKENVSYVNDLARQLTTLGIQLSPYNLNTLEDLNTRWKLLQVAIEDRIRQLHEAHRDFGPASQHFLSTSVQGPWERAISPNKVPYYINHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQVINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPVTLINFWPVDSALAEMENSNGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKQQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDTSTGLEEVMEQLNHSFPSSRGHNVGSLFHLADDLGRAMESLVSVMTDEEGAE

Target ORF information:

RefSeq Version	XM_014111346.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris dystrophin (DMD), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111346.1

ORF Insert Sequence:

ATGCTTTGGT GGGAAGAAGT AGAGGACTGT TATGAAAGAG AAGATGTTCA AAAGAAAACA 
TTCACAAAAT GGATAAATGC ACAGTTTTCT AAGTTTGGGA AGCAGCACAT AGAGAACCTC 
TTCAGTGACC TACAGGATGG GAGACGCCTC CTAGACCTTT TGGAAGGCCT GACAGGGCAA 
AAACTGCCAA AAGAAAAAGG ATCCACAAGA GTTCATGCCC TGAACAATGT CAACAAGGCA 
CTGCGCGTCT TGCAGAAAAA TAATGTTGAT TTAGTGAACA TTGGAAGTAC TGACATAGTA 
GATGGAAATC ACAAACTGAC TCTTGGTTTG ATTTGGAATA TAATCCTCCA CTGGCAGGTC 
AAAAATGTAA TGAAAAATAT CATGGCTGGA TTGCAACAAA CCAACAGTGA AAAGATTCTC 
CTGAGCTGGG TCCGACAATC AACTCGTAAT TATCCACAGG TTAATGTCAT TAACTTCACC 
ACCAGCTGGT CTGATGGCCT GGCTTTGAAC GCTCTCATCC ACAGTCATAG GCCAGACCTG 
TTTGATTGGA ATAGTGTGGT TTGCCAGCAG TCAGCCACAC AACGCCTGGA ACATGCATTC 
AACATTGCCA AATATCAATT AGGCATAGAG AAACTGCTTG ATCCTGAAGA TGTTGCCACC 
ACTTATCCAG ATAAGAAGTC CATCTTAATG TATATCACAT CACTCTTCCA AGTTTTGCCT 
CAACAAGTGA GCATTGAAGC CATCCAGGAA GTGGAAATGT TGCCAAGGCC ATCAAAAGTT 
ACTAGAGAAG AACATTTTCA ATTACATCAT CAAATGCACT ATTCTCAACA GATCACAGTC 
AGTCTAGCAC AGGGCTATGA ACGAGCCCCT TCCTCTCCTA AGCCTCGGTT CAAGAGCTAT 
GCCTACACAC AGGCTGCTTA TGTCACCACT TCTGACCCCA CACGGAGCCC ACTTCCTTCA 
CAGCATTTGG AAACTCCTGA AGACAAGTCA TTTGGCCGGT CATTGACAGA GACCGAAGCA 
AACCTGGACA GTTATCAAAC AGCTTTGGAA GAAGTACTCT CGTGGCTTCT TTCAGCTGAG 
GATGCACTGC AAGCCCAAGG AGAGATTTCT AATGATGTCG AAGAAGTGAA AGAACAATTT 
CATACTCATG AGGGATATAT GATGGACTTG ACATCCCATC AGGGACGGGT CGGTAATGTT 
CTCCAACTGG GAAGTCAACT GATTGGAACA GGGAAATTAT CAGAAGATGA AGAAACCGAA 
GTGCAGGAAC AAATGAATCT CCTCAATTCA AGATGGGAAT GCCTCAGGGT AGCTAGCATG 
GAAAAACAAA GCAATTTACA TAAAGTTCTA ATGGATCTCC AGAATCAGCA ACTGAAAGAG 
TTAAATGACT GGCTAACCAA AACAGAAGAG AGAACAAGGA AAATGGAGAA GGAGCCCCTT 
GGACCTGATA TTGAAGACCT AAAACGCCAA GTACAACAAC ATAAGGTGCT TCAAGAAGAC 
TTAGAACAGG AACAAGTCAG GGTCAATTCC CTCACTCATA TGGTGGTGGT AGTCGATGAA 
TCTAGTGGAG ACCATGCAAC TGCTGCTTTG GAAGAACAAC TTAAGGTACT GGGAGATCGA 
TGGGCAAACA TCTGTAGGTG GACAGAAGAT CGCTGGGTTC TTTTACAAGA CATCCTCCTA 
AAATGGCAGC GTTTTACTGA AGAACAGTGC CTTTTCAGTG CATGGCTTTC GGAGAAGGAA 
GATGCAGTGA ACAAGATTCA CACAACTGGC TTTAAGGATC AAAGTGAAGT GTTATCAAAT 
CTTCAGAAAC TGGCTGTCTT AAAAACAGAT CTGGAAAAGA AGAAGCAAAC CATGGACAAA 
CTCTGCTCAC TCAACCAAGA CCTTCTTTCA GCGCTGAAAA ACACAGTGGT AGCCCACAAG 
ATGGAAGCAT GGCTGGACAA CTTTGCCCAG CGCTGGGATA ATTTAGTCCA GAAACTTGAA 
AAAAGTTCAG CACAGATTTC ACAGGCTGTC ACCACCACTC AGCCATCACT AACACAGACA 
ACTGTAATGG AAACAGTAAC TATGGTGACC ACGAGGGAAC ACATCTTGGT AAAGCATGCC 
CAAGAGGAAC TGCCACCACC ACCCCCTCAG AAGAAGAGGC AGATTATCGT GGATTCTGAA 
ATTAGGAAAA GGTTGGATGT CGATATAACT GAACTTCACA GTTGGATTAC TCGTTCAGAA 
GCTGTGTTGC AGAGTCCTGA ATTTGCAATC TATCGGAAGG AAGGCAACTT CTCAGACCTT 
AAAGAAAAAG TCAATGCCAT AGAGCGAGAA AAAGCCGAGA AGTTCAGAAA ACTGCAAGAT 
GCCAGCAGAT CAGCTCAGGC CCTGGTGGAA CAGATGGTGA ATGAGGGTGT TAATGCTGAC 
AGCATCAAAC AAGCCTCCGA ACAACTGAAC AGCCGGTGGA TAGAGTTCTG CCAATTGCTA 
AGCGAGAGAC TTAACTGGCT GGAGTATCAG AACAACATCA TCACTTTCTA TAATCAGCTA 
CAACAATTGG AGCAGATGAC AACTACTGCT GAAAACTGGT TGAAAACCCA GCCTACCACC 
ACATCAGAGC CAACAGCAAT TAAAAGCCAG TTAAAAATTT GTAAGGATGA AATCAACCGA 
CTGTCAGCTC TTCAGCCTCA AATCGAGCGA TTAAAAATTC AAAGCATAGC CCTGAAAGAG 
AAAGGACAAG GGCCAATGTT CCTGGATGCA GACTTTGTGG CCTTTACAAA TCATTTTAAC 
CAAGTCTTTG CTGATGTGCA GGCAAGAGAA AAAGAGCTAC AAACAATTTT TGACAGTTTG 
CCACCCATGC GCTATCAGGA GACTATGAGT ACCATCCTGA CATGGATCCA GCAGTCAGAA 
ACCAAACTCT CTATACCTCA GGTTACTGTC ACTGAATATG ACATCATGGA ACAGAGACTC 
GGAGAGCTAC AGGCTTTACA AAGCTCTCTG CAAGAGCAAC AAAATGGCCT AAACTATCTC 
AGCACCACTG TGAAAGAGAT GTCAAAGAAA GCACCACTGT CTGATATTAG TCGGAAATAT 
CAATCAGAAT TTGAAGAGAT TGAGGGACGT TGGAAGAAGC TGTCTTCCCA GCTGGTTGAA 
CATTGTCAAA AGTTGGAGGA GCAAATGGCT AAACTTCGAA AAATTCAGAA TCACATAAAA 
ACTCTGAAGA AATGGATCAC TGAAGTCGAT GTTTTCCTGA AGGAGGAATG GCCTGCCCTT 
GGGGATTCAG AAATTCTGAA AAGACAGCTG AAACAGTGCA GGCTTTTAGT CAATGACATT 
CAGACCATCC AGCCTAGTCT CAACAGTGTC AATGAAGGGG CTCAGAAGAT GAAGAATGAA 
GCAGAACCAG AGTTTGCTGG CAGACTTGAG ACAGAGCTCC GAGAACTTAA CACCCAGTGG 
GATTACATGT GCCGCCAGGT CTATGCCAGG AAGGAAGCCT TAAAAGGAGG TTTGGATAAA 
ACTGTAAGTC TTCAGAAAGA TCTGTCAGAG ATGCATGAGT GGATGACACA AGCTGAAGAA 
GAATACCTAG AGAGAGATTT CGAATACAAG ACCCCTGATG AATTACAGAC AGCAGTTGAA 
GAGATGAAGA GAGCTAAAGA AGAGGCCCAG CAAAAAGAAG CAAAAGTGAA ACTCCTAACC 
GAGTCCGTCA ATAGTGTCAT AGCTCAGGCT CCACCTGCAG CACAAGAGGC CTTAAAAAAG 
GAACTTGACA CTCTCACCAC CAACTACCAG TGGCTCTGCA CCAGGCTCAA TGGCAAATGC 
AAGACCTTGG AAGAAGTTTG GGCGTGCTGG CATGAGTTAT TGTCCTACTT GGAGAAGGCA 
AACAAGTGGC TAAGTGAAGT AGAAGTCAAG CTTAAAACCA CTGAAAATAT TTCTGGGGGA 
GCTGAGGAAA TCGCCGAGGT GCTTGATTCG CTTGAAAATT TGATGCAACA TTCAGAGGAT 
AACCCGAATC AGATTCGCAT ATTGGCACAG ACCTTGACAG ATGGTGGAGT CATGGATGAA 
CTGATCAATG AGGAGCTTGA GACATTTAAT TCTCGTTGGA GAGAACTCCA TGAAGAGGCT 
GTGAGGAGGC AAAAGTTGCT TGAGCAGAGT ATCCAGTCGG CCCAGGAGAT AGAAAAATCC 
TTGCACTTAA TTCAGGAGTC CCTCTCTTCC ATTGACAAGC AGTTGGCAGC TTATATTGCT 
GACAAAGTGG ATGCAGCTCA GATGCCTCAG GAAGCCCAGA AAATCCAATC AGATTTGACA 
AGTCATGAGA TCAGTTTAGA AGAAATGAAG AAACATAACC AGGGAAAGGA GACTGCCCAA 
AGGGTACTAT CCCAAATTGA TGTGGCACAG AAAAAATTGC AGGATGTTTC CATGAAGTTT 
CGATTATTCC AGAAGCCAGC CAATTTTGAG CAGCGCCTAC AAGAAAGTAA AATGATTTTA 
GATGAAGTGA AGATGCATTT ACCTGCGTTG GAAACAAAGA GTGTGGAACA GGAAGTAGTA 
CAGTCACAGT TAAATCATTG TGTGAACTTG TATAAAAGTC TGAGTGAAGT GAAGTCTGAA 
GTGGAAATGG TAATAAAAAC TGGACGTCAG ATTGTACAGA AGAAGCAGAC GGAAAACCCG 
AAAGAGCTTG ATGAAAGAGT TACAGCTTTG AAATTGCATT ATAATGAGCT GGGAGCAAAG 
GTGACAGAAA GAAAGCAACA GTTGGAAAAA TGCTTGAAAT TGTCCCGTAA GATGCGAAAG 
GAAATGAATG CCCTGACAGA ATGGCTGGCA GCGACAGATA TGGAACTGAC GAAGAGATCG 
GCAGTTGAAG GAATGCCTAG TAATTTGGAT TCTGAAGTTG CCTGGGGAAA GGCTACTCAG 
AAAGAGATTG AGAAACAGAA GGTTCACCTA AAGAGTGTCA CAGAGGTAGG AGAGGCCTTG 
AAAACGGTTT TGGGCAAGAA GGAAATGTTG GTGGAAGATA AACTGAGTCT TCTGAATAGT 
AACTGGATAG CCGTCACTTC CCGAGCAGAA GAGTGGTTAA ACCTTTTATT GGAATACCAG 
AAACACATGG AAAACTTTGA CCAGAATGTG GATTACATCA CAAACTGGAT CATTCAGGCT 
GATGCACTTT TGGATGAATC TGAGAAAAAG AAACCTCAGC AAAAAGAAGA CATACTTAAG 
CGTTTAAAGG CTGAAATGAA TGACATACGT CCAAAGGTGG ATTCTACACG TGACCAAGCA 
GCAAACCTGA TGGCAAACCG CGGCGACCAC TGCAGGAAAG TAGTAGAGCC CAAAATCTCA 
GAGCTCAACC ATCGATTTGC AGCCATTTCT CACAGAATTA AGACTGGAAA GGCCTCCATT 
CCTTTGAAGG AATTGGAGCA GTTTAACTCA GATATACAAA AATTGCTTGA ACCACTGGAG 
GCTGAAATTC AGCAGGGGGT GAATCTGAAA GAGGAAGACT TCAATAAAGA TATGAGTGAA 
GACAATGAGG GTACTGTAAA AGAATTGTTG CAAAGAGGAG ACAACTTACA ACAAAGAATC 
ACAGATGAGA GAAAGCGAGA GGAAATAAAG ATAAAACAAC AGCTGTTACA GACAAAACAT 
AATGCTCTCA AGGATTTGAG GTCTCAAAGA AGAAAAAAGG CTCTAGAAAT TTCTCACCAG 
TGGTATCAGT ACAAGAGGCA GGCTGATGAT CTCCTGAAAT GCTTGGATGA CATTGAAAAA 
AAATTAGCCA GCCTACCTGA ACCCAGAGAT GAAAGGAAAA TAAAGGAAAT TGATCGTGAA 
TTGCAGAAGA AGAAAGAGGA GCTGAATGCA GTGCGTAGGC AAGCTGAGGG CTTGTCTGAG 
GATGGGGCCG CAATGGCAGT GGAGCCAACT CAGATCCAGC TCAGCAAGCG CTGGCGGGAA 
ATTGAGAGCA AATTTGCTCA GTTTCGAAGA CTCAACTTTG CACAAATTCA CACTGTCCAT 
GAAGAGTCAG TGGTGGCGAT GACTGAAGAC ATGCCTTTGG AAATTTCTTA TGTGCCTTCT 
ACTTACCTGA CTGAGATCAC TCATGTCTCA CAAGCCCTAT CAGAAGTGGA AGAGCTTCTT 
AATGCTCCCG ACCTTTGTGC TCAAGATTTT GAAGATCTCT TTAAACAAGA GGAATCCTTG 
AAGAACATAA AAGACAGCCT GCAACAAATC TCAGGTCGGA TTGACATCAT TCACAATAAA 
AAGACAGCAG CATTGCACAG TGCCACTCCT GCAGAAAGGG CAAAGCTCCA GGAAGCTCTC 
TCACGGCTTG ATTTCCAATG GGAAAGAGTT AACAATATGT ACAAGGACCG ACAAGGGAGA 
TTTGACAGAT CTGTGGAAAA ATGGCGGCGG TTTCATTATG ATATGAAGAT ACTTAATCAA 
TGGCTAACAG AAGCTGAACA GTTTCTCAAA AAGACACAAA TTCCTGAGAA TTGGGAACAT 
GCCAAATACA AATGGTATCT TAAGGAACTC CAGGATGGCA TTGGACAGCG GCAAAGTGTT 
GTCAGGGTAT TGAATGCAAC TGGGGAAGAA ATAATTCAAC AGTCCTCAAA AACAGATGCC 
AGTATTCTCC AAGAAAAACT GGGAAGCCTG AATCTGCGGT GGCAGGAGGT CTGCAAACAG 
CTGGCAGAAA GAAAAAAGAG GCTAGAGGAA CAGAAGAATA TCTTGTCAGA ATTTCAAAGA 
GATGTAAATG AATTTGTTTT ATGGTTGGAA GAAGCGGATA ACGTTGCTAA TATTCCACTT 
GAACCTGGAA ATGAGCAGCA GCTAAAAGAA AAACTTGAAC AAGTCAAGTT ACTGGCAGAA 
GAGTTGCCCC TGCGCCAGGG AATTCTAAAA CAATTAAATG AAACTGGAGG AACAGTGCTT 
GTAAGTGCTC CCCTAAGCCC AGAAGAGCAA GATAAACTTG AAAATAAGCT CAAGCAGACA 
AATCTTCAGT GGATAAAGGT TTCTAGAAAT CTGCCTGAGA AGCAAGAAGA AATTGAGGCA 
CACGTAAAAG ACCTTGGACA GCTGGAAGAG CAGTTAAATC ATCTGCTTCT ATGGCTGTCT 
CCTATTAGGA ATCAGTTGGA AATTTACAAT CAGCCAAATC AAACAGGACC ATTTGACATC 
AAGGAAATTG AAGTAGCAGT TCAAGCTAAA CAGCCGGATG TGGAAGGGAT TTTGTCTAAA 
GGGCAGCATT TGTACAAGGA AAAACCAGCC ACTCAGCCAG CGAAGAGAAA GCTGGAAGAT 
CTCAGCTCTG ATTGGAAGGT GGTAACTCAG TTGCTTCAAG AGCTGCGGGC AAAGCAGCCT 
GGCCCAGCTC CTGGACTGAC CACTGTCAGA GCCCCTCCCA GTCAGACTGT TACTCTGGTG 
ACACAACCCG CGGTTACCAA GGAAACTGCC ATCTCCAAAC TAGAAATGCC ATCTTCATTG 
CTGTTGGAGG TACCTGCACT GGCAGATTTC AACCGAGCTT GGACAGAACT TACCGACTGG 
CTGTCTCTGC TTGATCGAGT TATAAAATCA CAGAGGGTGA TGGTGGGTGA TCTTGAAGAC 
ATTAACGAGA TGATCATCAA GCAGAAGGCA ACGCTGCAGG ATTTGGAACA GAGGCGCCCC 
CAGTTGGAAG AACTCATTAC CGCTGCCCAG AATTTGAAAA ACAAGACCAG CAATCAAGAG 
GCTAGAACAA TCATTACTGA TCGAATTGAA AGAATTCAGA GTCAGTGGGA TGAAGTACAG 
GAACATCTTC AGAACCGGAG GCAACAGTTG AATGAAATGT TAAAGGATTC CACACAATGG 
CTGGAAGCTA AAGAGGAGGC TGAGCAGGTG TTGGGGCAGG CCAGAGCCAA GCTTGAGTCA 
TGGAAGGAGG CTCCCTACAC AGTAGATGCA ATCCAAAAGA AAATCACAGA AACCAAGCAG 
TTGGCCAAAG ACCTCCGCCA GTGGCAGATA AATGTAGATG TTGCAAATGA TTTGGCACTG 
AAACTTCTCC GAGATTATTC TGCAGATGAT ACCAGAAAAG TACACATGAT AACAGAGAAC 
ATCAATGCCT CTTGGGCAAG CATCCATAAA AGATTGAGTG AGCGAGAGGC TGCTCTGGAA 
GAAACCCACA GATTACTGCA ACAGTTCCCC TTGGACCTGG AGAAGTTCCT TGCCTGGCTT 
ACAGAAGCCG AAACAACTGC CAACGTCCTG CAGGATGCCA CCCATAAGGA AAGGCTTCTA 
GAAGATTCCA AGGGAGTAAG AGAGCTGATG AAACAATGGC AAGATCTCCA AGGAGAAATC 
GAAGCTCACA CAGATATCTA TCACAACCTG GACGAAAATG GCCAAAAAGT CCTGAGATCC 
CTGGAAGGTT CTGACGATGC AGCCTTGTTG CAAAGACGTT TGGATAACAT GAACTTCAAG 
TGGAGCGAAC TTCGGAAAAA GTCTCTCAAC ATTAGGTCTC ACTTGGAAGC CAGTTCTGAC 
CAGTGGAAGC GTCTGCACCT TTCTCTTCAG GAACTTCTGG TATGGCTCCA GCTGAAAGAT 
GATGAGTTAA GCCGGCAGGC ACCCATTGGA GGAGACTTTC CAGCGGTGCA GAAGCAGAAT 
GATGTACACA GGGCCTTCAA GAGGGAATTG AAAACGAAAG AACCTGTAAT CATGAGTACT 
CTTGAGACTG TACGAATATT TCTGACAGAG CAGCCTTTAG AAGGACTAGA GAAACTCTAC 
CAGGAGCCCA GAGAGCTGCC TCCTGAAGAG AGAGCCCAGA ATGTCACACG GCTCCTACGA 
AAGCAAGCTG AGGAGGTCAA CACTCAGTGG GAAAAACTGA ACGTGCACTC TGCAGACTGG 
CAGAGAAAAA TAGACGAGGC CCTCGAAAGA CTCCAGGAGC TTCAGGAAGC AACAGATGAG 
CTGGATCTCA AACTACGTCA GGCAGAGGTG ATCAAAGGAT CCTGGCAGCC TGTGGGTGAT 
CTCCTCATTG ACTCTCTCCA AGATCACCTC GAAAAAGTCA AGGCGCTTCG AGGAGAAATT 
ACACCTCTGA AAGAGAATGT CAGCTACGTC AATGACCTTG CTCGCCAACT CACTACGTTG 
GGCATTCAGC TGTCACCATA TAACCTCAAC ACTCTGGAAG ACCTGAACAC CAGATGGAAG 
CTTCTGCAGG TGGCCATTGA GGACCGCATC AGGCAGCTGC ATGAAGCGCA CAGGGACTTT 
GGACCAGCCT CCCAGCACTT CCTTTCCACT TCTGTCCAGG GTCCCTGGGA GAGAGCCATC 
TCACCAAACA AAGTGCCCTA CTATATCAAC CACGAGACCC AAACAACTTG CTGGGACCAT 
CCCAAAATGA CAGAGCTCTA CCAGTCTTTA GCTGACCTGA ATAATGTCAG GTTCTCAGCT 
TACAGGACTG CCATGAAACT CCGAAGACTG CAGAAGGCCC TTTGCTTGGA TCTCTTGAGC 
CTATCGGCTG CATGCGATGC CTTGGACCAG CACAACCTCA AGCAAAATGA CCAGCCCATG 
GATATCCTGC AGGTCATTAA CTGTCTGACC ACTATTTATG ATCGCCTAGA GCAAGAGCAC 
AACAATCTGG TCAACGTCCC TCTCTGCGTG GATATGTGTC TCAATTGGCT GCTGAATGTT 
TATGACACGG GACGAACGGG GAGGATCCGG GTCCTGTCTT TTAAAACTGG CATCATTTCT 
CTGTGTAAAG CCCATTTGGA AGACAAGTAC AGATACCTCT TCAAGCAAGT GGCAAGTTCG 
ACAGGATTTT GTGACCAGCG CAGGCTGGGC CTCCTCCTGC ATGACTCTAT CCAGATCCCA 
AGACAGTTGG GTGAAGTCGC ATCGTTCGGG GGCAGTAACA TTGAGCCGAG TGTCAGGAGC 
TGCTTCCAGT TTGCTAATAA TAAGCCTGAG ATCGAAGCGG CCCTCTTCCT AGACTGGATG 
CGCCTGGAGC CCCAGTCCAT GGTGTGGCTG CCTGTCCTGC ACCGAGTGGC TGCCGCGGAA 
ACTGCCAAGC ACCAGGCCAA GTGCAACATC TGCAAGGAGT GTCCCATCAT CGGATTCAGG 
TACAGGAGTC TAAAGCACTT TAATTATGAC ATCTGCCAAA GTTGCTTTTT TTCTGGTCGA 
GTTGCAAAAG GCCATAAAAT GCACTATCCC ATGGTGGAAT ACTGCACTCC GACTACATCG 
GGAGAAGATG TCCGTGACTT TGCCAAGGTA CTAAAAAACA AATTTCGAAC CAAAAGGTAT 
TTTGCGAAGC ATCCCCGAAT GGGCTACCTG CCAGTGCAGA CTGTCTTAGA GGGGGACAAC 
ATGGAAACTC CTGTCACTCT GATCAACTTC TGGCCGGTAG ATTCTGCGCT AGCAGAAATG 
GAAAACAGCA ATGGATCTTA TCTAAATGAT AGCATCTCTC CTAATGAGAG CATAGATGAT 
GAACATTTGT TAATCCAGCA TTACTGCCAA AGTTTGAACC AGGACTCCCC CCTGAGCCAG 
CCTCGTAGTC CTGCCCAGAT CTTGATTTCC TTAGAGAGTG AGGAAAGAGG GGAGCTAGAG 
AGAATCCTAG CAGATCTTGA GGAAGAAAAC AGAAATCTGC AAGCAGAGTA TGATCGTCTA 
AAGCAGCAGC ATGAACACAA AGGCCTGTCC CCACTGCCAT CCCCTCCTGA AATGATGCCT 
ACTTCTCCCC AAAGTCCCCG GGATGCTGAG CTCATCGCTG AGGCCAAGCT GCTGCGTCAA 
CACAAAGGCC GCCTGGAAGC CAGGATGCAA ATCCTTGAAG ACCATAACAA ACAACTGGAA 
TCCCAGTTAC ACAGGCTCAG GCAGCTGCTG GAGCAACCCC AGGCAGAGGC CAAGGTGAAT 
GGTACAACGG TGTCTTCTCC TTCTACCTCT CTTCAGAGGT CAGATAGCAG TCAGCCTATG 
CTGCTCCGGG TGGTCGGCAG TCAGACTTCA GAATCCATGG GCGAGGAAGA CCTGCTCAGC 
CCTCCCCAGG ACACAAGCAC AGGGTTAGAG GAAGTGATGG AGCAGCTCAA CCACTCCTTC 
CCTAGTTCCA GAGGACACAA TGTAGGAAGC CTTTTCCACT TGGCAGATGA TTTGGGCAGA 
GCGATGGAGT CCTTAGTTTC AGTCATGACA GATGAAGAAG GGGCAGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMD ( XM_014111346.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013966821.1 Canis lupus familiaris dystrophin (DMD), transcript variant X7, mRNA.

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CloneID	OCe45417
Clone ID Related Accession (Same CDS sequence)	XM_014111346.1 , XM_014111346.2
Accession Version	XM_014111346.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11031bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	dystrophin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014111346.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTGGT GGGAAGAAGT AGAGGACTGT TATGAAAGAG AAGATGTTCA AAAGAAAACA 
TTCACAAAAT GGATAAATGC ACAGTTTTCT AAGTTTGGGA AGCAGCACAT AGAGAACCTC 
TTCAGTGACC TACAGGATGG GAGACGCCTC CTAGACCTTT TGGAAGGCCT GACAGGGCAA 
AAACTGCCAA AAGAAAAAGG ATCCACAAGA GTTCATGCCC TGAACAATGT CAACAAGGCA 
CTGCGCGTCT TGCAGAAAAA TAATGTTGAT TTAGTGAACA TTGGAAGTAC TGACATAGTA 
GATGGAAATC ACAAACTGAC TCTTGGTTTG ATTTGGAATA TAATCCTCCA CTGGCAGGTC 
AAAAATGTAA TGAAAAATAT CATGGCTGGA TTGCAACAAA CCAACAGTGA AAAGATTCTC 
CTGAGCTGGG TCCGACAATC AACTCGTAAT TATCCACAGG TTAATGTCAT TAACTTCACC 
ACCAGCTGGT CTGATGGCCT GGCTTTGAAC GCTCTCATCC ACAGTCATAG GCCAGACCTG 
TTTGATTGGA ATAGTGTGGT TTGCCAGCAG TCAGCCACAC AACGCCTGGA ACATGCATTC 
AACATTGCCA AATATCAATT AGGCATAGAG AAACTGCTTG ATCCTGAAGA TGTTGCCACC 
ACTTATCCAG ATAAGAAGTC CATCTTAATG TATATCACAT CACTCTTCCA AGTTTTGCCT 
CAACAAGTGA GCATTGAAGC CATCCAGGAA GTGGAAATGT TGCCAAGGCC ATCAAAAGTT 
ACTAGAGAAG AACATTTTCA ATTACATCAT CAAATGCACT ATTCTCAACA GATCACAGTC 
AGTCTAGCAC AGGGCTATGA ACGAGCCCCT TCCTCTCCTA AGCCTCGGTT CAAGAGCTAT 
GCCTACACAC AGGCTGCTTA TGTCACCACT TCTGACCCCA CACGGAGCCC ACTTCCTTCA 
CAGCATTTGG AAACTCCTGA AGACAAGTCA TTTGGCCGGT CATTGACAGA GACCGAAGCA 
AACCTGGACA GTTATCAAAC AGCTTTGGAA GAAGTACTCT CGTGGCTTCT TTCAGCTGAG 
GATGCACTGC AAGCCCAAGG AGAGATTTCT AATGATGTCG AAGAAGTGAA AGAACAATTT 
CATACTCATG AGGGATATAT GATGGACTTG ACATCCCATC AGGGACGGGT CGGTAATGTT 
CTCCAACTGG GAAGTCAACT GATTGGAACA GGGAAATTAT CAGAAGATGA AGAAACCGAA 
GTGCAGGAAC AAATGAATCT CCTCAATTCA AGATGGGAAT GCCTCAGGGT AGCTAGCATG 
GAAAAACAAA GCAATTTACA TAAAGTTCTA ATGGATCTCC AGAATCAGCA ACTGAAAGAG 
TTAAATGACT GGCTAACCAA AACAGAAGAG AGAACAAGGA AAATGGAGAA GGAGCCCCTT 
GGACCTGATA TTGAAGACCT AAAACGCCAA GTACAACAAC ATAAGGTGCT TCAAGAAGAC 
TTAGAACAGG AACAAGTCAG GGTCAATTCC CTCACTCATA TGGTGGTGGT AGTCGATGAA 
TCTAGTGGAG ACCATGCAAC TGCTGCTTTG GAAGAACAAC TTAAGGTACT GGGAGATCGA 
TGGGCAAACA TCTGTAGGTG GACAGAAGAT CGCTGGGTTC TTTTACAAGA CATCCTCCTA 
AAATGGCAGC GTTTTACTGA AGAACAGTGC CTTTTCAGTG CATGGCTTTC GGAGAAGGAA 
GATGCAGTGA ACAAGATTCA CACAACTGGC TTTAAGGATC AAAGTGAAGT GTTATCAAAT 
CTTCAGAAAC TGGCTGTCTT AAAAACAGAT CTGGAAAAGA AGAAGCAAAC CATGGACAAA 
CTCTGCTCAC TCAACCAAGA CCTTCTTTCA GCGCTGAAAA ACACAGTGGT AGCCCACAAG 
ATGGAAGCAT GGCTGGACAA CTTTGCCCAG CGCTGGGATA ATTTAGTCCA GAAACTTGAA 
AAAAGTTCAG CACAGATTTC ACAGGCTGTC ACCACCACTC AGCCATCACT AACACAGACA 
ACTGTAATGG AAACAGTAAC TATGGTGACC ACGAGGGAAC ACATCTTGGT AAAGCATGCC 
CAAGAGGAAC TGCCACCACC ACCCCCTCAG AAGAAGAGGC AGATTATCGT GGATTCTGAA 
ATTAGGAAAA GGTTGGATGT CGATATAACT GAACTTCACA GTTGGATTAC TCGTTCAGAA 
GCTGTGTTGC AGAGTCCTGA ATTTGCAATC TATCGGAAGG AAGGCAACTT CTCAGACCTT 
AAAGAAAAAG TCAATGCCAT AGAGCGAGAA AAAGCCGAGA AGTTCAGAAA ACTGCAAGAT 
GCCAGCAGAT CAGCTCAGGC CCTGGTGGAA CAGATGGTGA ATGAGGGTGT TAATGCTGAC 
AGCATCAAAC AAGCCTCCGA ACAACTGAAC AGCCGGTGGA TAGAGTTCTG CCAATTGCTA 
AGCGAGAGAC TTAACTGGCT GGAGTATCAG AACAACATCA TCACTTTCTA TAATCAGCTA 
CAACAATTGG AGCAGATGAC AACTACTGCT GAAAACTGGT TGAAAACCCA GCCTACCACC 
ACATCAGAGC CAACAGCAAT TAAAAGCCAG TTAAAAATTT GTAAGGATGA AATCAACCGA 
CTGTCAGCTC TTCAGCCTCA AATCGAGCGA TTAAAAATTC AAAGCATAGC CCTGAAAGAG 
AAAGGACAAG GGCCAATGTT CCTGGATGCA GACTTTGTGG CCTTTACAAA TCATTTTAAC 
CAAGTCTTTG CTGATGTGCA GGCAAGAGAA AAAGAGCTAC AAACAATTTT TGACAGTTTG 
CCACCCATGC GCTATCAGGA GACTATGAGT ACCATCCTGA CATGGATCCA GCAGTCAGAA 
ACCAAACTCT CTATACCTCA GGTTACTGTC ACTGAATATG ACATCATGGA ACAGAGACTC 
GGAGAGCTAC AGGCTTTACA AAGCTCTCTG CAAGAGCAAC AAAATGGCCT AAACTATCTC 
AGCACCACTG TGAAAGAGAT GTCAAAGAAA GCACCACTGT CTGATATTAG TCGGAAATAT 
CAATCAGAAT TTGAAGAGAT TGAGGGACGT TGGAAGAAGC TGTCTTCCCA GCTGGTTGAA 
CATTGTCAAA AGTTGGAGGA GCAAATGGCT AAACTTCGAA AAATTCAGAA TCACATAAAA 
ACTCTGAAGA AATGGATCAC TGAAGTCGAT GTTTTCCTGA AGGAGGAATG GCCTGCCCTT 
GGGGATTCAG AAATTCTGAA AAGACAGCTG AAACAGTGCA GGCTTTTAGT CAATGACATT 
CAGACCATCC AGCCTAGTCT CAACAGTGTC AATGAAGGGG CTCAGAAGAT GAAGAATGAA 
GCAGAACCAG AGTTTGCTGG CAGACTTGAG ACAGAGCTCC GAGAACTTAA CACCCAGTGG 
GATTACATGT GCCGCCAGGT CTATGCCAGG AAGGAAGCCT TAAAAGGAGG TTTGGATAAA 
ACTGTAAGTC TTCAGAAAGA TCTGTCAGAG ATGCATGAGT GGATGACACA AGCTGAAGAA 
GAATACCTAG AGAGAGATTT CGAATACAAG ACCCCTGATG AATTACAGAC AGCAGTTGAA 
GAGATGAAGA GAGCTAAAGA AGAGGCCCAG CAAAAAGAAG CAAAAGTGAA ACTCCTAACC 
GAGTCCGTCA ATAGTGTCAT AGCTCAGGCT CCACCTGCAG CACAAGAGGC CTTAAAAAAG 
GAACTTGACA CTCTCACCAC CAACTACCAG TGGCTCTGCA CCAGGCTCAA TGGCAAATGC 
AAGACCTTGG AAGAAGTTTG GGCGTGCTGG CATGAGTTAT TGTCCTACTT GGAGAAGGCA 
AACAAGTGGC TAAGTGAAGT AGAAGTCAAG CTTAAAACCA CTGAAAATAT TTCTGGGGGA 
GCTGAGGAAA TCGCCGAGGT GCTTGATTCG CTTGAAAATT TGATGCAACA TTCAGAGGAT 
AACCCGAATC AGATTCGCAT ATTGGCACAG ACCTTGACAG ATGGTGGAGT CATGGATGAA 
CTGATCAATG AGGAGCTTGA GACATTTAAT TCTCGTTGGA GAGAACTCCA TGAAGAGGCT 
GTGAGGAGGC AAAAGTTGCT TGAGCAGAGT ATCCAGTCGG CCCAGGAGAT AGAAAAATCC 
TTGCACTTAA TTCAGGAGTC CCTCTCTTCC ATTGACAAGC AGTTGGCAGC TTATATTGCT 
GACAAAGTGG ATGCAGCTCA GATGCCTCAG GAAGCCCAGA AAATCCAATC AGATTTGACA 
AGTCATGAGA TCAGTTTAGA AGAAATGAAG AAACATAACC AGGGAAAGGA GACTGCCCAA 
AGGGTACTAT CCCAAATTGA TGTGGCACAG AAAAAATTGC AGGATGTTTC CATGAAGTTT 
CGATTATTCC AGAAGCCAGC CAATTTTGAG CAGCGCCTAC AAGAAAGTAA AATGATTTTA 
GATGAAGTGA AGATGCATTT ACCTGCGTTG GAAACAAAGA GTGTGGAACA GGAAGTAGTA 
CAGTCACAGT TAAATCATTG TGTGAACTTG TATAAAAGTC TGAGTGAAGT GAAGTCTGAA 
GTGGAAATGG TAATAAAAAC TGGACGTCAG ATTGTACAGA AGAAGCAGAC GGAAAACCCG 
AAAGAGCTTG ATGAAAGAGT TACAGCTTTG AAATTGCATT ATAATGAGCT GGGAGCAAAG 
GTGACAGAAA GAAAGCAACA GTTGGAAAAA TGCTTGAAAT TGTCCCGTAA GATGCGAAAG 
GAAATGAATG CCCTGACAGA ATGGCTGGCA GCGACAGATA TGGAACTGAC GAAGAGATCG 
GCAGTTGAAG GAATGCCTAG TAATTTGGAT TCTGAAGTTG CCTGGGGAAA GGCTACTCAG 
AAAGAGATTG AGAAACAGAA GGTTCACCTA AAGAGTGTCA CAGAGGTAGG AGAGGCCTTG 
AAAACGGTTT TGGGCAAGAA GGAAATGTTG GTGGAAGATA AACTGAGTCT TCTGAATAGT 
AACTGGATAG CCGTCACTTC CCGAGCAGAA GAGTGGTTAA ACCTTTTATT GGAATACCAG 
AAACACATGG AAAACTTTGA CCAGAATGTG GATTACATCA CAAACTGGAT CATTCAGGCT 
GATGCACTTT TGGATGAATC TGAGAAAAAG AAACCTCAGC AAAAAGAAGA CATACTTAAG 
CGTTTAAAGG CTGAAATGAA TGACATACGT CCAAAGGTGG ATTCTACACG TGACCAAGCA 
GCAAACCTGA TGGCAAACCG CGGCGACCAC TGCAGGAAAG TAGTAGAGCC CAAAATCTCA 
GAGCTCAACC ATCGATTTGC AGCCATTTCT CACAGAATTA AGACTGGAAA GGCCTCCATT 
CCTTTGAAGG AATTGGAGCA GTTTAACTCA GATATACAAA AATTGCTTGA ACCACTGGAG 
GCTGAAATTC AGCAGGGGGT GAATCTGAAA GAGGAAGACT TCAATAAAGA TATGAGTGAA 
GACAATGAGG GTACTGTAAA AGAATTGTTG CAAAGAGGAG ACAACTTACA ACAAAGAATC 
ACAGATGAGA GAAAGCGAGA GGAAATAAAG ATAAAACAAC AGCTGTTACA GACAAAACAT 
AATGCTCTCA AGGATTTGAG GTCTCAAAGA AGAAAAAAGG CTCTAGAAAT TTCTCACCAG 
TGGTATCAGT ACAAGAGGCA GGCTGATGAT CTCCTGAAAT GCTTGGATGA CATTGAAAAA 
AAATTAGCCA GCCTACCTGA ACCCAGAGAT GAAAGGAAAA TAAAGGAAAT TGATCGTGAA 
TTGCAGAAGA AGAAAGAGGA GCTGAATGCA GTGCGTAGGC AAGCTGAGGG CTTGTCTGAG 
GATGGGGCCG CAATGGCAGT GGAGCCAACT CAGATCCAGC TCAGCAAGCG CTGGCGGGAA 
ATTGAGAGCA AATTTGCTCA GTTTCGAAGA CTCAACTTTG CACAAATTCA CACTGTCCAT 
GAAGAGTCAG TGGTGGCGAT GACTGAAGAC ATGCCTTTGG AAATTTCTTA TGTGCCTTCT 
ACTTACCTGA CTGAGATCAC TCATGTCTCA CAAGCCCTAT CAGAAGTGGA AGAGCTTCTT 
AATGCTCCCG ACCTTTGTGC TCAAGATTTT GAAGATCTCT TTAAACAAGA GGAATCCTTG 
AAGAACATAA AAGACAGCCT GCAACAAATC TCAGGTCGGA TTGACATCAT TCACAATAAA 
AAGACAGCAG CATTGCACAG TGCCACTCCT GCAGAAAGGG CAAAGCTCCA GGAAGCTCTC 
TCACGGCTTG ATTTCCAATG GGAAAGAGTT AACAATATGT ACAAGGACCG ACAAGGGAGA 
TTTGACAGAT CTGTGGAAAA ATGGCGGCGG TTTCATTATG ATATGAAGAT ACTTAATCAA 
TGGCTAACAG AAGCTGAACA GTTTCTCAAA AAGACACAAA TTCCTGAGAA TTGGGAACAT 
GCCAAATACA AATGGTATCT TAAGGAACTC CAGGATGGCA TTGGACAGCG GCAAAGTGTT 
GTCAGGGTAT TGAATGCAAC TGGGGAAGAA ATAATTCAAC AGTCCTCAAA AACAGATGCC 
AGTATTCTCC AAGAAAAACT GGGAAGCCTG AATCTGCGGT GGCAGGAGGT CTGCAAACAG 
CTGGCAGAAA GAAAAAAGAG GCTAGAGGAA CAGAAGAATA TCTTGTCAGA ATTTCAAAGA 
GATGTAAATG AATTTGTTTT ATGGTTGGAA GAAGCGGATA ACGTTGCTAA TATTCCACTT 
GAACCTGGAA ATGAGCAGCA GCTAAAAGAA AAACTTGAAC AAGTCAAGTT ACTGGCAGAA 
GAGTTGCCCC TGCGCCAGGG AATTCTAAAA CAATTAAATG AAACTGGAGG AACAGTGCTT 
GTAAGTGCTC CCCTAAGCCC AGAAGAGCAA GATAAACTTG AAAATAAGCT CAAGCAGACA 
AATCTTCAGT GGATAAAGGT TTCTAGAAAT CTGCCTGAGA AGCAAGAAGA AATTGAGGCA 
CACGTAAAAG ACCTTGGACA GCTGGAAGAG CAGTTAAATC ATCTGCTTCT ATGGCTGTCT 
CCTATTAGGA ATCAGTTGGA AATTTACAAT CAGCCAAATC AAACAGGACC ATTTGACATC 
AAGGAAATTG AAGTAGCAGT TCAAGCTAAA CAGCCGGATG TGGAAGGGAT TTTGTCTAAA 
GGGCAGCATT TGTACAAGGA AAAACCAGCC ACTCAGCCAG CGAAGAGAAA GCTGGAAGAT 
CTCAGCTCTG ATTGGAAGGT GGTAACTCAG TTGCTTCAAG AGCTGCGGGC AAAGCAGCCT 
GGCCCAGCTC CTGGACTGAC CACTGTCAGA GCCCCTCCCA GTCAGACTGT TACTCTGGTG 
ACACAACCCG CGGTTACCAA GGAAACTGCC ATCTCCAAAC TAGAAATGCC ATCTTCATTG 
CTGTTGGAGG TACCTGCACT GGCAGATTTC AACCGAGCTT GGACAGAACT TACCGACTGG 
CTGTCTCTGC TTGATCGAGT TATAAAATCA CAGAGGGTGA TGGTGGGTGA TCTTGAAGAC 
ATTAACGAGA TGATCATCAA GCAGAAGGCA ACGCTGCAGG ATTTGGAACA GAGGCGCCCC 
CAGTTGGAAG AACTCATTAC CGCTGCCCAG AATTTGAAAA ACAAGACCAG CAATCAAGAG 
GCTAGAACAA TCATTACTGA TCGAATTGAA AGAATTCAGA GTCAGTGGGA TGAAGTACAG 
GAACATCTTC AGAACCGGAG GCAACAGTTG AATGAAATGT TAAAGGATTC CACACAATGG 
CTGGAAGCTA AAGAGGAGGC TGAGCAGGTG TTGGGGCAGG CCAGAGCCAA GCTTGAGTCA 
TGGAAGGAGG CTCCCTACAC AGTAGATGCA ATCCAAAAGA AAATCACAGA AACCAAGCAG 
TTGGCCAAAG ACCTCCGCCA GTGGCAGATA AATGTAGATG TTGCAAATGA TTTGGCACTG 
AAACTTCTCC GAGATTATTC TGCAGATGAT ACCAGAAAAG TACACATGAT AACAGAGAAC 
ATCAATGCCT CTTGGGCAAG CATCCATAAA AGATTGAGTG AGCGAGAGGC TGCTCTGGAA 
GAAACCCACA GATTACTGCA ACAGTTCCCC TTGGACCTGG AGAAGTTCCT TGCCTGGCTT 
ACAGAAGCCG AAACAACTGC CAACGTCCTG CAGGATGCCA CCCATAAGGA AAGGCTTCTA 
GAAGATTCCA AGGGAGTAAG AGAGCTGATG AAACAATGGC AAGATCTCCA AGGAGAAATC 
GAAGCTCACA CAGATATCTA TCACAACCTG GACGAAAATG GCCAAAAAGT CCTGAGATCC 
CTGGAAGGTT CTGACGATGC AGCCTTGTTG CAAAGACGTT TGGATAACAT GAACTTCAAG 
TGGAGCGAAC TTCGGAAAAA GTCTCTCAAC ATTAGGTCTC ACTTGGAAGC CAGTTCTGAC 
CAGTGGAAGC GTCTGCACCT TTCTCTTCAG GAACTTCTGG TATGGCTCCA GCTGAAAGAT 
GATGAGTTAA GCCGGCAGGC ACCCATTGGA GGAGACTTTC CAGCGGTGCA GAAGCAGAAT 
GATGTACACA GGGCCTTCAA GAGGGAATTG AAAACGAAAG AACCTGTAAT CATGAGTACT 
CTTGAGACTG TACGAATATT TCTGACAGAG CAGCCTTTAG AAGGACTAGA GAAACTCTAC 
CAGGAGCCCA GAGAGCTGCC TCCTGAAGAG AGAGCCCAGA ATGTCACACG GCTCCTACGA 
AAGCAAGCTG AGGAGGTCAA CACTCAGTGG GAAAAACTGA ACGTGCACTC TGCAGACTGG 
CAGAGAAAAA TAGACGAGGC CCTCGAAAGA CTCCAGGAGC TTCAGGAAGC AACAGATGAG 
CTGGATCTCA AACTACGTCA GGCAGAGGTG ATCAAAGGAT CCTGGCAGCC TGTGGGTGAT 
CTCCTCATTG ACTCTCTCCA AGATCACCTC GAAAAAGTCA AGGCGCTTCG AGGAGAAATT 
ACACCTCTGA AAGAGAATGT CAGCTACGTC AATGACCTTG CTCGCCAACT CACTACGTTG 
GGCATTCAGC TGTCACCATA TAACCTCAAC ACTCTGGAAG ACCTGAACAC CAGATGGAAG 
CTTCTGCAGG TGGCCATTGA GGACCGCATC AGGCAGCTGC ATGAAGCGCA CAGGGACTTT 
GGACCAGCCT CCCAGCACTT CCTTTCCACT TCTGTCCAGG GTCCCTGGGA GAGAGCCATC 
TCACCAAACA AAGTGCCCTA CTATATCAAC CACGAGACCC AAACAACTTG CTGGGACCAT 
CCCAAAATGA CAGAGCTCTA CCAGTCTTTA GCTGACCTGA ATAATGTCAG GTTCTCAGCT 
TACAGGACTG CCATGAAACT CCGAAGACTG CAGAAGGCCC TTTGCTTGGA TCTCTTGAGC 
CTATCGGCTG CATGCGATGC CTTGGACCAG CACAACCTCA AGCAAAATGA CCAGCCCATG 
GATATCCTGC AGGTCATTAA CTGTCTGACC ACTATTTATG ATCGCCTAGA GCAAGAGCAC 
AACAATCTGG TCAACGTCCC TCTCTGCGTG GATATGTGTC TCAATTGGCT GCTGAATGTT 
TATGACACGG GACGAACGGG GAGGATCCGG GTCCTGTCTT TTAAAACTGG CATCATTTCT 
CTGTGTAAAG CCCATTTGGA AGACAAGTAC AGATACCTCT TCAAGCAAGT GGCAAGTTCG 
ACAGGATTTT GTGACCAGCG CAGGCTGGGC CTCCTCCTGC ATGACTCTAT CCAGATCCCA 
AGACAGTTGG GTGAAGTCGC ATCGTTCGGG GGCAGTAACA TTGAGCCGAG TGTCAGGAGC 
TGCTTCCAGT TTGCTAATAA TAAGCCTGAG ATCGAAGCGG CCCTCTTCCT AGACTGGATG 
CGCCTGGAGC CCCAGTCCAT GGTGTGGCTG CCTGTCCTGC ACCGAGTGGC TGCCGCGGAA 
ACTGCCAAGC ACCAGGCCAA GTGCAACATC TGCAAGGAGT GTCCCATCAT CGGATTCAGG 
TACAGGAGTC TAAAGCACTT TAATTATGAC ATCTGCCAAA GTTGCTTTTT TTCTGGTCGA 
GTTGCAAAAG GCCATAAAAT GCACTATCCC ATGGTGGAAT ACTGCACTCC GACTACATCG 
GGAGAAGATG TCCGTGACTT TGCCAAGGTA CTAAAAAACA AATTTCGAAC CAAAAGGTAT 
TTTGCGAAGC ATCCCCGAAT GGGCTACCTG CCAGTGCAGA CTGTCTTAGA GGGGGACAAC 
ATGGAAACTC CTGTCACTCT GATCAACTTC TGGCCGGTAG ATTCTGCGCT AGCAGAAATG 
GAAAACAGCA ATGGATCTTA TCTAAATGAT AGCATCTCTC CTAATGAGAG CATAGATGAT 
GAACATTTGT TAATCCAGCA TTACTGCCAA AGTTTGAACC AGGACTCCCC CCTGAGCCAG 
CCTCGTAGTC CTGCCCAGAT CTTGATTTCC TTAGAGAGTG AGGAAAGAGG GGAGCTAGAG 
AGAATCCTAG CAGATCTTGA GGAAGAAAAC AGAAATCTGC AAGCAGAGTA TGATCGTCTA 
AAGCAGCAGC ATGAACACAA AGGCCTGTCC CCACTGCCAT CCCCTCCTGA AATGATGCCT 
ACTTCTCCCC AAAGTCCCCG GGATGCTGAG CTCATCGCTG AGGCCAAGCT GCTGCGTCAA 
CACAAAGGCC GCCTGGAAGC CAGGATGCAA ATCCTTGAAG ACCATAACAA ACAACTGGAA 
TCCCAGTTAC ACAGGCTCAG GCAGCTGCTG GAGCAACCCC AGGCAGAGGC CAAGGTGAAT 
GGTACAACGG TGTCTTCTCC TTCTACCTCT CTTCAGAGGT CAGATAGCAG TCAGCCTATG 
CTGCTCCGGG TGGTCGGCAG TCAGACTTCA GAATCCATGG GCGAGGAAGA CCTGCTCAGC 
CCTCCCCAGG ACACAAGCAC AGGGTTAGAG GAAGTGATGG AGCAGCTCAA CCACTCCTTC 
CCTAGTTCCA GAGGACACAA TGTAGGAAGC CTTTTCCACT TGGCAGATGA TTTGGGCAGA 
GCGATGGAGT CCTTAGTTTC AGTCATGACA GATGAAGAAG GGGCAGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013966821.1
CDS	247..11277
Translation	MLWWEEVEDCYEREDVQKKTFTKWINAQFSKFGKQHIENLFSDLQDGRRLLDLLEGLTGQKLPKEKGSTRVHALNNVNKALRVLQKNNVDLVNIGSTDIVDGNHKLTLGLIWNIILHWQVKNVMKNIMAGLQQTNSEKILLSWVRQSTRNYPQVNVINFTTSWSDGLALNALIHSHRPDLFDWNSVVCQQSATQRLEHAFNIAKYQLGIEKLLDPEDVATTYPDKKSILMYITSLFQVLPQQVSIEAIQEVEMLPRPSKVTREEHFQLHHQMHYSQQITVSLAQGYERAPSSPKPRFKSYAYTQAAYVTTSDPTRSPLPSQHLETPEDKSFGRSLTETEANLDSYQTALEEVLSWLLSAEDALQAQGEISNDVEEVKEQFHTHEGYMMDLTSHQGRVGNVLQLGSQLIGTGKLSEDEETEVQEQMNLLNSRWECLRVASMEKQSNLHKVLMDLQNQQLKELNDWLTKTEERTRKMEKEPLGPDIEDLKRQVQQHKVLQEDLEQEQVRVNSLTHMVVVVDESSGDHATAALEEQLKVLGDRWANICRWTEDRWVLLQDILLKWQRFTEEQCLFSAWLSEKEDAVNKIHTTGFKDQSEVLSNLQKLAVLKTDLEKKKQTMDKLCSLNQDLLSALKNTVVAHKMEAWLDNFAQRWDNLVQKLEKSSAQISQAVTTTQPSLTQTTVMETVTMVTTREHILVKHAQEELPPPPPQKKRQIIVDSEIRKRLDVDITELHSWITRSEAVLQSPEFAIYRKEGNFSDLKEKVNAIEREKAEKFRKLQDASRSAQALVEQMVNEGVNADSIKQASEQLNSRWIEFCQLLSERLNWLEYQNNIITFYNQLQQLEQMTTTAENWLKTQPTTTSEPTAIKSQLKICKDEINRLSALQPQIERLKIQSIALKEKGQGPMFLDADFVAFTNHFNQVFADVQAREKELQTIFDSLPPMRYQETMSTILTWIQQSETKLSIPQVTVTEYDIMEQRLGELQALQSSLQEQQNGLNYLSTTVKEMSKKAPLSDISRKYQSEFEEIEGRWKKLSSQLVEHCQKLEEQMAKLRKIQNHIKTLKKWITEVDVFLKEEWPALGDSEILKRQLKQCRLLVNDIQTIQPSLNSVNEGAQKMKNEAEPEFAGRLETELRELNTQWDYMCRQVYARKEALKGGLDKTVSLQKDLSEMHEWMTQAEEEYLERDFEYKTPDELQTAVEEMKRAKEEAQQKEAKVKLLTESVNSVIAQAPPAAQEALKKELDTLTTNYQWLCTRLNGKCKTLEEVWACWHELLSYLEKANKWLSEVEVKLKTTENISGGAEEIAEVLDSLENLMQHSEDNPNQIRILAQTLTDGGVMDELINEELETFNSRWRELHEEAVRRQKLLEQSIQSAQEIEKSLHLIQESLSSIDKQLAAYIADKVDAAQMPQEAQKIQSDLTSHEISLEEMKKHNQGKETAQRVLSQIDVAQKKLQDVSMKFRLFQKPANFEQRLQESKMILDEVKMHLPALETKSVEQEVVQSQLNHCVNLYKSLSEVKSEVEMVIKTGRQIVQKKQTENPKELDERVTALKLHYNELGAKVTERKQQLEKCLKLSRKMRKEMNALTEWLAATDMELTKRSAVEGMPSNLDSEVAWGKATQKEIEKQKVHLKSVTEVGEALKTVLGKKEMLVEDKLSLLNSNWIAVTSRAEEWLNLLLEYQKHMENFDQNVDYITNWIIQADALLDESEKKKPQQKEDILKRLKAEMNDIRPKVDSTRDQAANLMANRGDHCRKVVEPKISELNHRFAAISHRIKTGKASIPLKELEQFNSDIQKLLEPLEAEIQQGVNLKEEDFNKDMSEDNEGTVKELLQRGDNLQQRITDERKREEIKIKQQLLQTKHNALKDLRSQRRKKALEISHQWYQYKRQADDLLKCLDDIEKKLASLPEPRDERKIKEIDRELQKKKEELNAVRRQAEGLSEDGAAMAVEPTQIQLSKRWREIESKFAQFRRLNFAQIHTVHEESVVAMTEDMPLEISYVPSTYLTEITHVSQALSEVEELLNAPDLCAQDFEDLFKQEESLKNIKDSLQQISGRIDIIHNKKTAALHSATPAERAKLQEALSRLDFQWERVNNMYKDRQGRFDRSVEKWRRFHYDMKILNQWLTEAEQFLKKTQIPENWEHAKYKWYLKELQDGIGQRQSVVRVLNATGEEIIQQSSKTDASILQEKLGSLNLRWQEVCKQLAERKKRLEEQKNILSEFQRDVNEFVLWLEEADNVANIPLEPGNEQQLKEKLEQVKLLAEELPLRQGILKQLNETGGTVLVSAPLSPEEQDKLENKLKQTNLQWIKVSRNLPEKQEEIEAHVKDLGQLEEQLNHLLLWLSPIRNQLEIYNQPNQTGPFDIKEIEVAVQAKQPDVEGILSKGQHLYKEKPATQPAKRKLEDLSSDWKVVTQLLQELRAKQPGPAPGLTTVRAPPSQTVTLVTQPAVTKETAISKLEMPSSLLLEVPALADFNRAWTELTDWLSLLDRVIKSQRVMVGDLEDINEMIIKQKATLQDLEQRRPQLEELITAAQNLKNKTSNQEARTIITDRIERIQSQWDEVQEHLQNRRQQLNEMLKDSTQWLEAKEEAEQVLGQARAKLESWKEAPYTVDAIQKKITETKQLAKDLRQWQINVDVANDLALKLLRDYSADDTRKVHMITENINASWASIHKRLSEREAALEETHRLLQQFPLDLEKFLAWLTEAETTANVLQDATHKERLLEDSKGVRELMKQWQDLQGEIEAHTDIYHNLDENGQKVLRSLEGSDDAALLQRRLDNMNFKWSELRKKSLNIRSHLEASSDQWKRLHLSLQELLVWLQLKDDELSRQAPIGGDFPAVQKQNDVHRAFKRELKTKEPVIMSTLETVRIFLTEQPLEGLEKLYQEPRELPPEERAQNVTRLLRKQAEEVNTQWEKLNVHSADWQRKIDEALERLQELQEATDELDLKLRQAEVIKGSWQPVGDLLIDSLQDHLEKVKALRGEITPLKENVSYVNDLARQLTTLGIQLSPYNLNTLEDLNTRWKLLQVAIEDRIRQLHEAHRDFGPASQHFLSTSVQGPWERAISPNKVPYYINHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQVINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPVTLINFWPVDSALAEMENSNGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKQQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDTSTGLEEVMEQLNHSFPSSRGHNVGSLFHLADDLGRAMESLVSVMTDEEGAE

Target ORF information:

RefSeq Version	XM_014111346.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris dystrophin (DMD), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111346.2

ORF Insert Sequence:

ATGCTTTGGT GGGAAGAAGT AGAGGACTGT TATGAAAGAG AAGATGTTCA AAAGAAAACA 
TTCACAAAAT GGATAAATGC ACAGTTTTCT AAGTTTGGGA AGCAGCACAT AGAGAACCTC 
TTCAGTGACC TACAGGATGG GAGACGCCTC CTAGACCTTT TGGAAGGCCT GACAGGGCAA 
AAACTGCCAA AAGAAAAAGG ATCCACAAGA GTTCATGCCC TGAACAATGT CAACAAGGCA 
CTGCGCGTCT TGCAGAAAAA TAATGTTGAT TTAGTGAACA TTGGAAGTAC TGACATAGTA 
GATGGAAATC ACAAACTGAC TCTTGGTTTG ATTTGGAATA TAATCCTCCA CTGGCAGGTC 
AAAAATGTAA TGAAAAATAT CATGGCTGGA TTGCAACAAA CCAACAGTGA AAAGATTCTC 
CTGAGCTGGG TCCGACAATC AACTCGTAAT TATCCACAGG TTAATGTCAT TAACTTCACC 
ACCAGCTGGT CTGATGGCCT GGCTTTGAAC GCTCTCATCC ACAGTCATAG GCCAGACCTG 
TTTGATTGGA ATAGTGTGGT TTGCCAGCAG TCAGCCACAC AACGCCTGGA ACATGCATTC 
AACATTGCCA AATATCAATT AGGCATAGAG AAACTGCTTG ATCCTGAAGA TGTTGCCACC 
ACTTATCCAG ATAAGAAGTC CATCTTAATG TATATCACAT CACTCTTCCA AGTTTTGCCT 
CAACAAGTGA GCATTGAAGC CATCCAGGAA GTGGAAATGT TGCCAAGGCC ATCAAAAGTT 
ACTAGAGAAG AACATTTTCA ATTACATCAT CAAATGCACT ATTCTCAACA GATCACAGTC 
AGTCTAGCAC AGGGCTATGA ACGAGCCCCT TCCTCTCCTA AGCCTCGGTT CAAGAGCTAT 
GCCTACACAC AGGCTGCTTA TGTCACCACT TCTGACCCCA CACGGAGCCC ACTTCCTTCA 
CAGCATTTGG AAACTCCTGA AGACAAGTCA TTTGGCCGGT CATTGACAGA GACCGAAGCA 
AACCTGGACA GTTATCAAAC AGCTTTGGAA GAAGTACTCT CGTGGCTTCT TTCAGCTGAG 
GATGCACTGC AAGCCCAAGG AGAGATTTCT AATGATGTCG AAGAAGTGAA AGAACAATTT 
CATACTCATG AGGGATATAT GATGGACTTG ACATCCCATC AGGGACGGGT CGGTAATGTT 
CTCCAACTGG GAAGTCAACT GATTGGAACA GGGAAATTAT CAGAAGATGA AGAAACCGAA 
GTGCAGGAAC AAATGAATCT CCTCAATTCA AGATGGGAAT GCCTCAGGGT AGCTAGCATG 
GAAAAACAAA GCAATTTACA TAAAGTTCTA ATGGATCTCC AGAATCAGCA ACTGAAAGAG 
TTAAATGACT GGCTAACCAA AACAGAAGAG AGAACAAGGA AAATGGAGAA GGAGCCCCTT 
GGACCTGATA TTGAAGACCT AAAACGCCAA GTACAACAAC ATAAGGTGCT TCAAGAAGAC 
TTAGAACAGG AACAAGTCAG GGTCAATTCC CTCACTCATA TGGTGGTGGT AGTCGATGAA 
TCTAGTGGAG ACCATGCAAC TGCTGCTTTG GAAGAACAAC TTAAGGTACT GGGAGATCGA 
TGGGCAAACA TCTGTAGGTG GACAGAAGAT CGCTGGGTTC TTTTACAAGA CATCCTCCTA 
AAATGGCAGC GTTTTACTGA AGAACAGTGC CTTTTCAGTG CATGGCTTTC GGAGAAGGAA 
GATGCAGTGA ACAAGATTCA CACAACTGGC TTTAAGGATC AAAGTGAAGT GTTATCAAAT 
CTTCAGAAAC TGGCTGTCTT AAAAACAGAT CTGGAAAAGA AGAAGCAAAC CATGGACAAA 
CTCTGCTCAC TCAACCAAGA CCTTCTTTCA GCGCTGAAAA ACACAGTGGT AGCCCACAAG 
ATGGAAGCAT GGCTGGACAA CTTTGCCCAG CGCTGGGATA ATTTAGTCCA GAAACTTGAA 
AAAAGTTCAG CACAGATTTC ACAGGCTGTC ACCACCACTC AGCCATCACT AACACAGACA 
ACTGTAATGG AAACAGTAAC TATGGTGACC ACGAGGGAAC ACATCTTGGT AAAGCATGCC 
CAAGAGGAAC TGCCACCACC ACCCCCTCAG AAGAAGAGGC AGATTATCGT GGATTCTGAA 
ATTAGGAAAA GGTTGGATGT CGATATAACT GAACTTCACA GTTGGATTAC TCGTTCAGAA 
GCTGTGTTGC AGAGTCCTGA ATTTGCAATC TATCGGAAGG AAGGCAACTT CTCAGACCTT 
AAAGAAAAAG TCAATGCCAT AGAGCGAGAA AAAGCCGAGA AGTTCAGAAA ACTGCAAGAT 
GCCAGCAGAT CAGCTCAGGC CCTGGTGGAA CAGATGGTGA ATGAGGGTGT TAATGCTGAC 
AGCATCAAAC AAGCCTCCGA ACAACTGAAC AGCCGGTGGA TAGAGTTCTG CCAATTGCTA 
AGCGAGAGAC TTAACTGGCT GGAGTATCAG AACAACATCA TCACTTTCTA TAATCAGCTA 
CAACAATTGG AGCAGATGAC AACTACTGCT GAAAACTGGT TGAAAACCCA GCCTACCACC 
ACATCAGAGC CAACAGCAAT TAAAAGCCAG TTAAAAATTT GTAAGGATGA AATCAACCGA 
CTGTCAGCTC TTCAGCCTCA AATCGAGCGA TTAAAAATTC AAAGCATAGC CCTGAAAGAG 
AAAGGACAAG GGCCAATGTT CCTGGATGCA GACTTTGTGG CCTTTACAAA TCATTTTAAC 
CAAGTCTTTG CTGATGTGCA GGCAAGAGAA AAAGAGCTAC AAACAATTTT TGACAGTTTG 
CCACCCATGC GCTATCAGGA GACTATGAGT ACCATCCTGA CATGGATCCA GCAGTCAGAA 
ACCAAACTCT CTATACCTCA GGTTACTGTC ACTGAATATG ACATCATGGA ACAGAGACTC 
GGAGAGCTAC AGGCTTTACA AAGCTCTCTG CAAGAGCAAC AAAATGGCCT AAACTATCTC 
AGCACCACTG TGAAAGAGAT GTCAAAGAAA GCACCACTGT CTGATATTAG TCGGAAATAT 
CAATCAGAAT TTGAAGAGAT TGAGGGACGT TGGAAGAAGC TGTCTTCCCA GCTGGTTGAA 
CATTGTCAAA AGTTGGAGGA GCAAATGGCT AAACTTCGAA AAATTCAGAA TCACATAAAA 
ACTCTGAAGA AATGGATCAC TGAAGTCGAT GTTTTCCTGA AGGAGGAATG GCCTGCCCTT 
GGGGATTCAG AAATTCTGAA AAGACAGCTG AAACAGTGCA GGCTTTTAGT CAATGACATT 
CAGACCATCC AGCCTAGTCT CAACAGTGTC AATGAAGGGG CTCAGAAGAT GAAGAATGAA 
GCAGAACCAG AGTTTGCTGG CAGACTTGAG ACAGAGCTCC GAGAACTTAA CACCCAGTGG 
GATTACATGT GCCGCCAGGT CTATGCCAGG AAGGAAGCCT TAAAAGGAGG TTTGGATAAA 
ACTGTAAGTC TTCAGAAAGA TCTGTCAGAG ATGCATGAGT GGATGACACA AGCTGAAGAA 
GAATACCTAG AGAGAGATTT CGAATACAAG ACCCCTGATG AATTACAGAC AGCAGTTGAA 
GAGATGAAGA GAGCTAAAGA AGAGGCCCAG CAAAAAGAAG CAAAAGTGAA ACTCCTAACC 
GAGTCCGTCA ATAGTGTCAT AGCTCAGGCT CCACCTGCAG CACAAGAGGC CTTAAAAAAG 
GAACTTGACA CTCTCACCAC CAACTACCAG TGGCTCTGCA CCAGGCTCAA TGGCAAATGC 
AAGACCTTGG AAGAAGTTTG GGCGTGCTGG CATGAGTTAT TGTCCTACTT GGAGAAGGCA 
AACAAGTGGC TAAGTGAAGT AGAAGTCAAG CTTAAAACCA CTGAAAATAT TTCTGGGGGA 
GCTGAGGAAA TCGCCGAGGT GCTTGATTCG CTTGAAAATT TGATGCAACA TTCAGAGGAT 
AACCCGAATC AGATTCGCAT ATTGGCACAG ACCTTGACAG ATGGTGGAGT CATGGATGAA 
CTGATCAATG AGGAGCTTGA GACATTTAAT TCTCGTTGGA GAGAACTCCA TGAAGAGGCT 
GTGAGGAGGC AAAAGTTGCT TGAGCAGAGT ATCCAGTCGG CCCAGGAGAT AGAAAAATCC 
TTGCACTTAA TTCAGGAGTC CCTCTCTTCC ATTGACAAGC AGTTGGCAGC TTATATTGCT 
GACAAAGTGG ATGCAGCTCA GATGCCTCAG GAAGCCCAGA AAATCCAATC AGATTTGACA 
AGTCATGAGA TCAGTTTAGA AGAAATGAAG AAACATAACC AGGGAAAGGA GACTGCCCAA 
AGGGTACTAT CCCAAATTGA TGTGGCACAG AAAAAATTGC AGGATGTTTC CATGAAGTTT 
CGATTATTCC AGAAGCCAGC CAATTTTGAG CAGCGCCTAC AAGAAAGTAA AATGATTTTA 
GATGAAGTGA AGATGCATTT ACCTGCGTTG GAAACAAAGA GTGTGGAACA GGAAGTAGTA 
CAGTCACAGT TAAATCATTG TGTGAACTTG TATAAAAGTC TGAGTGAAGT GAAGTCTGAA 
GTGGAAATGG TAATAAAAAC TGGACGTCAG ATTGTACAGA AGAAGCAGAC GGAAAACCCG 
AAAGAGCTTG ATGAAAGAGT TACAGCTTTG AAATTGCATT ATAATGAGCT GGGAGCAAAG 
GTGACAGAAA GAAAGCAACA GTTGGAAAAA TGCTTGAAAT TGTCCCGTAA GATGCGAAAG 
GAAATGAATG CCCTGACAGA ATGGCTGGCA GCGACAGATA TGGAACTGAC GAAGAGATCG 
GCAGTTGAAG GAATGCCTAG TAATTTGGAT TCTGAAGTTG CCTGGGGAAA GGCTACTCAG 
AAAGAGATTG AGAAACAGAA GGTTCACCTA AAGAGTGTCA CAGAGGTAGG AGAGGCCTTG 
AAAACGGTTT TGGGCAAGAA GGAAATGTTG GTGGAAGATA AACTGAGTCT TCTGAATAGT 
AACTGGATAG CCGTCACTTC CCGAGCAGAA GAGTGGTTAA ACCTTTTATT GGAATACCAG 
AAACACATGG AAAACTTTGA CCAGAATGTG GATTACATCA CAAACTGGAT CATTCAGGCT 
GATGCACTTT TGGATGAATC TGAGAAAAAG AAACCTCAGC AAAAAGAAGA CATACTTAAG 
CGTTTAAAGG CTGAAATGAA TGACATACGT CCAAAGGTGG ATTCTACACG TGACCAAGCA 
GCAAACCTGA TGGCAAACCG CGGCGACCAC TGCAGGAAAG TAGTAGAGCC CAAAATCTCA 
GAGCTCAACC ATCGATTTGC AGCCATTTCT CACAGAATTA AGACTGGAAA GGCCTCCATT 
CCTTTGAAGG AATTGGAGCA GTTTAACTCA GATATACAAA AATTGCTTGA ACCACTGGAG 
GCTGAAATTC AGCAGGGGGT GAATCTGAAA GAGGAAGACT TCAATAAAGA TATGAGTGAA 
GACAATGAGG GTACTGTAAA AGAATTGTTG CAAAGAGGAG ACAACTTACA ACAAAGAATC 
ACAGATGAGA GAAAGCGAGA GGAAATAAAG ATAAAACAAC AGCTGTTACA GACAAAACAT 
AATGCTCTCA AGGATTTGAG GTCTCAAAGA AGAAAAAAGG CTCTAGAAAT TTCTCACCAG 
TGGTATCAGT ACAAGAGGCA GGCTGATGAT CTCCTGAAAT GCTTGGATGA CATTGAAAAA 
AAATTAGCCA GCCTACCTGA ACCCAGAGAT GAAAGGAAAA TAAAGGAAAT TGATCGTGAA 
TTGCAGAAGA AGAAAGAGGA GCTGAATGCA GTGCGTAGGC AAGCTGAGGG CTTGTCTGAG 
GATGGGGCCG CAATGGCAGT GGAGCCAACT CAGATCCAGC TCAGCAAGCG CTGGCGGGAA 
ATTGAGAGCA AATTTGCTCA GTTTCGAAGA CTCAACTTTG CACAAATTCA CACTGTCCAT 
GAAGAGTCAG TGGTGGCGAT GACTGAAGAC ATGCCTTTGG AAATTTCTTA TGTGCCTTCT 
ACTTACCTGA CTGAGATCAC TCATGTCTCA CAAGCCCTAT CAGAAGTGGA AGAGCTTCTT 
AATGCTCCCG ACCTTTGTGC TCAAGATTTT GAAGATCTCT TTAAACAAGA GGAATCCTTG 
AAGAACATAA AAGACAGCCT GCAACAAATC TCAGGTCGGA TTGACATCAT TCACAATAAA 
AAGACAGCAG CATTGCACAG TGCCACTCCT GCAGAAAGGG CAAAGCTCCA GGAAGCTCTC 
TCACGGCTTG ATTTCCAATG GGAAAGAGTT AACAATATGT ACAAGGACCG ACAAGGGAGA 
TTTGACAGAT CTGTGGAAAA ATGGCGGCGG TTTCATTATG ATATGAAGAT ACTTAATCAA 
TGGCTAACAG AAGCTGAACA GTTTCTCAAA AAGACACAAA TTCCTGAGAA TTGGGAACAT 
GCCAAATACA AATGGTATCT TAAGGAACTC CAGGATGGCA TTGGACAGCG GCAAAGTGTT 
GTCAGGGTAT TGAATGCAAC TGGGGAAGAA ATAATTCAAC AGTCCTCAAA AACAGATGCC 
AGTATTCTCC AAGAAAAACT GGGAAGCCTG AATCTGCGGT GGCAGGAGGT CTGCAAACAG 
CTGGCAGAAA GAAAAAAGAG GCTAGAGGAA CAGAAGAATA TCTTGTCAGA ATTTCAAAGA 
GATGTAAATG AATTTGTTTT ATGGTTGGAA GAAGCGGATA ACGTTGCTAA TATTCCACTT 
GAACCTGGAA ATGAGCAGCA GCTAAAAGAA AAACTTGAAC AAGTCAAGTT ACTGGCAGAA 
GAGTTGCCCC TGCGCCAGGG AATTCTAAAA CAATTAAATG AAACTGGAGG AACAGTGCTT 
GTAAGTGCTC CCCTAAGCCC AGAAGAGCAA GATAAACTTG AAAATAAGCT CAAGCAGACA 
AATCTTCAGT GGATAAAGGT TTCTAGAAAT CTGCCTGAGA AGCAAGAAGA AATTGAGGCA 
CACGTAAAAG ACCTTGGACA GCTGGAAGAG CAGTTAAATC ATCTGCTTCT ATGGCTGTCT 
CCTATTAGGA ATCAGTTGGA AATTTACAAT CAGCCAAATC AAACAGGACC ATTTGACATC 
AAGGAAATTG AAGTAGCAGT TCAAGCTAAA CAGCCGGATG TGGAAGGGAT TTTGTCTAAA 
GGGCAGCATT TGTACAAGGA AAAACCAGCC ACTCAGCCAG CGAAGAGAAA GCTGGAAGAT 
CTCAGCTCTG ATTGGAAGGT GGTAACTCAG TTGCTTCAAG AGCTGCGGGC AAAGCAGCCT 
GGCCCAGCTC CTGGACTGAC CACTGTCAGA GCCCCTCCCA GTCAGACTGT TACTCTGGTG 
ACACAACCCG CGGTTACCAA GGAAACTGCC ATCTCCAAAC TAGAAATGCC ATCTTCATTG 
CTGTTGGAGG TACCTGCACT GGCAGATTTC AACCGAGCTT GGACAGAACT TACCGACTGG 
CTGTCTCTGC TTGATCGAGT TATAAAATCA CAGAGGGTGA TGGTGGGTGA TCTTGAAGAC 
ATTAACGAGA TGATCATCAA GCAGAAGGCA ACGCTGCAGG ATTTGGAACA GAGGCGCCCC 
CAGTTGGAAG AACTCATTAC CGCTGCCCAG AATTTGAAAA ACAAGACCAG CAATCAAGAG 
GCTAGAACAA TCATTACTGA TCGAATTGAA AGAATTCAGA GTCAGTGGGA TGAAGTACAG 
GAACATCTTC AGAACCGGAG GCAACAGTTG AATGAAATGT TAAAGGATTC CACACAATGG 
CTGGAAGCTA AAGAGGAGGC TGAGCAGGTG TTGGGGCAGG CCAGAGCCAA GCTTGAGTCA 
TGGAAGGAGG CTCCCTACAC AGTAGATGCA ATCCAAAAGA AAATCACAGA AACCAAGCAG 
TTGGCCAAAG ACCTCCGCCA GTGGCAGATA AATGTAGATG TTGCAAATGA TTTGGCACTG 
AAACTTCTCC GAGATTATTC TGCAGATGAT ACCAGAAAAG TACACATGAT AACAGAGAAC 
ATCAATGCCT CTTGGGCAAG CATCCATAAA AGATTGAGTG AGCGAGAGGC TGCTCTGGAA 
GAAACCCACA GATTACTGCA ACAGTTCCCC TTGGACCTGG AGAAGTTCCT TGCCTGGCTT 
ACAGAAGCCG AAACAACTGC CAACGTCCTG CAGGATGCCA CCCATAAGGA AAGGCTTCTA 
GAAGATTCCA AGGGAGTAAG AGAGCTGATG AAACAATGGC AAGATCTCCA AGGAGAAATC 
GAAGCTCACA CAGATATCTA TCACAACCTG GACGAAAATG GCCAAAAAGT CCTGAGATCC 
CTGGAAGGTT CTGACGATGC AGCCTTGTTG CAAAGACGTT TGGATAACAT GAACTTCAAG 
TGGAGCGAAC TTCGGAAAAA GTCTCTCAAC ATTAGGTCTC ACTTGGAAGC CAGTTCTGAC 
CAGTGGAAGC GTCTGCACCT TTCTCTTCAG GAACTTCTGG TATGGCTCCA GCTGAAAGAT 
GATGAGTTAA GCCGGCAGGC ACCCATTGGA GGAGACTTTC CAGCGGTGCA GAAGCAGAAT 
GATGTACACA GGGCCTTCAA GAGGGAATTG AAAACGAAAG AACCTGTAAT CATGAGTACT 
CTTGAGACTG TACGAATATT TCTGACAGAG CAGCCTTTAG AAGGACTAGA GAAACTCTAC 
CAGGAGCCCA GAGAGCTGCC TCCTGAAGAG AGAGCCCAGA ATGTCACACG GCTCCTACGA 
AAGCAAGCTG AGGAGGTCAA CACTCAGTGG GAAAAACTGA ACGTGCACTC TGCAGACTGG 
CAGAGAAAAA TAGACGAGGC CCTCGAAAGA CTCCAGGAGC TTCAGGAAGC AACAGATGAG 
CTGGATCTCA AACTACGTCA GGCAGAGGTG ATCAAAGGAT CCTGGCAGCC TGTGGGTGAT 
CTCCTCATTG ACTCTCTCCA AGATCACCTC GAAAAAGTCA AGGCGCTTCG AGGAGAAATT 
ACACCTCTGA AAGAGAATGT CAGCTACGTC AATGACCTTG CTCGCCAACT CACTACGTTG 
GGCATTCAGC TGTCACCATA TAACCTCAAC ACTCTGGAAG ACCTGAACAC CAGATGGAAG 
CTTCTGCAGG TGGCCATTGA GGACCGCATC AGGCAGCTGC ATGAAGCGCA CAGGGACTTT 
GGACCAGCCT CCCAGCACTT CCTTTCCACT TCTGTCCAGG GTCCCTGGGA GAGAGCCATC 
TCACCAAACA AAGTGCCCTA CTATATCAAC CACGAGACCC AAACAACTTG CTGGGACCAT 
CCCAAAATGA CAGAGCTCTA CCAGTCTTTA GCTGACCTGA ATAATGTCAG GTTCTCAGCT 
TACAGGACTG CCATGAAACT CCGAAGACTG CAGAAGGCCC TTTGCTTGGA TCTCTTGAGC 
CTATCGGCTG CATGCGATGC CTTGGACCAG CACAACCTCA AGCAAAATGA CCAGCCCATG 
GATATCCTGC AGGTCATTAA CTGTCTGACC ACTATTTATG ATCGCCTAGA GCAAGAGCAC 
AACAATCTGG TCAACGTCCC TCTCTGCGTG GATATGTGTC TCAATTGGCT GCTGAATGTT 
TATGACACGG GACGAACGGG GAGGATCCGG GTCCTGTCTT TTAAAACTGG CATCATTTCT 
CTGTGTAAAG CCCATTTGGA AGACAAGTAC AGATACCTCT TCAAGCAAGT GGCAAGTTCG 
ACAGGATTTT GTGACCAGCG CAGGCTGGGC CTCCTCCTGC ATGACTCTAT CCAGATCCCA 
AGACAGTTGG GTGAAGTCGC ATCGTTCGGG GGCAGTAACA TTGAGCCGAG TGTCAGGAGC 
TGCTTCCAGT TTGCTAATAA TAAGCCTGAG ATCGAAGCGG CCCTCTTCCT AGACTGGATG 
CGCCTGGAGC CCCAGTCCAT GGTGTGGCTG CCTGTCCTGC ACCGAGTGGC TGCCGCGGAA 
ACTGCCAAGC ACCAGGCCAA GTGCAACATC TGCAAGGAGT GTCCCATCAT CGGATTCAGG 
TACAGGAGTC TAAAGCACTT TAATTATGAC ATCTGCCAAA GTTGCTTTTT TTCTGGTCGA 
GTTGCAAAAG GCCATAAAAT GCACTATCCC ATGGTGGAAT ACTGCACTCC GACTACATCG 
GGAGAAGATG TCCGTGACTT TGCCAAGGTA CTAAAAAACA AATTTCGAAC CAAAAGGTAT 
TTTGCGAAGC ATCCCCGAAT GGGCTACCTG CCAGTGCAGA CTGTCTTAGA GGGGGACAAC 
ATGGAAACTC CTGTCACTCT GATCAACTTC TGGCCGGTAG ATTCTGCGCT AGCAGAAATG 
GAAAACAGCA ATGGATCTTA TCTAAATGAT AGCATCTCTC CTAATGAGAG CATAGATGAT 
GAACATTTGT TAATCCAGCA TTACTGCCAA AGTTTGAACC AGGACTCCCC CCTGAGCCAG 
CCTCGTAGTC CTGCCCAGAT CTTGATTTCC TTAGAGAGTG AGGAAAGAGG GGAGCTAGAG 
AGAATCCTAG CAGATCTTGA GGAAGAAAAC AGAAATCTGC AAGCAGAGTA TGATCGTCTA 
AAGCAGCAGC ATGAACACAA AGGCCTGTCC CCACTGCCAT CCCCTCCTGA AATGATGCCT 
ACTTCTCCCC AAAGTCCCCG GGATGCTGAG CTCATCGCTG AGGCCAAGCT GCTGCGTCAA 
CACAAAGGCC GCCTGGAAGC CAGGATGCAA ATCCTTGAAG ACCATAACAA ACAACTGGAA 
TCCCAGTTAC ACAGGCTCAG GCAGCTGCTG GAGCAACCCC AGGCAGAGGC CAAGGTGAAT 
GGTACAACGG TGTCTTCTCC TTCTACCTCT CTTCAGAGGT CAGATAGCAG TCAGCCTATG 
CTGCTCCGGG TGGTCGGCAG TCAGACTTCA GAATCCATGG GCGAGGAAGA CCTGCTCAGC 
CCTCCCCAGG ACACAAGCAC AGGGTTAGAG GAAGTGATGG AGCAGCTCAA CCACTCCTTC 
CCTAGTTCCA GAGGACACAA TGTAGGAAGC CTTTTCCACT TGGCAGATGA TTTGGGCAGA 
GCGATGGAGT CCTTAGTTTC AGTCATGACA GATGAAGAAG GGGCAGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMD ( XM_014111347.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013966822.1 Canis lupus familiaris dystrophin (DMD), transcript variant X8, mRNA.

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CloneID	OCe45418
Clone ID Related Accession (Same CDS sequence)	XM_014111347.1 , XM_014111347.2
Accession Version	XM_014111347.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	dystrophin isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTGGT GGGAAGAAGT AGAGGACTGT TATGAAAGAG AAGATGTTCA AAAGAAAACA 
TTCACAAAAT GGATAAATGC ACAGTTTTCT AAGTTTGGGA AGCAGCACAT AGAGAACCTC 
TTCAGTGACC TACAGGATGG GAGACGCCTC CTAGACCTTT TGGAAGGCCT GACAGGGCAA 
AAACTGCCAA AAGAAAAAGG ATCCACAAGA GTTCATGCCC TGAACAATGT CAACAAGGCA 
CTGCGCGTCT TGCAGAAAAA TAATGTTGAT TTAGTGAACA TTGGAAGTAC TGACATAGTA 
GATGGAAATC ACAAACTGAC TCTTGGTTTG ATTTGGAATA TAATCCTCCA CTGGCAGGTC 
AAAAATGTAA TGAAAAATAT CATGGCTGGA TTGCAACAAA CCAACAGTGA AAAGATTCTC 
CTGAGCTGGG TCCGACAATC AACTCGTAAT TATCCACAGG TTAATGTCAT TAACTTCACC 
ACCAGCTGGT CTGATGGCCT GGCTTTGAAC GCTCTCATCC ACAGTCATAG GCCAGACCTG 
TTTGATTGGA ATAGTGTGGT TTGCCAGCAG TCAGCCACAC AACGCCTGGA ACATGCATTC 
AACATTGCCA AATATCAATT AGGCATAGAG AAACTGCTTG ATCCTGAAGA TGTTGCCACC 
ACTTATCCAG ATAAGAAGTC CATCTTAATG TATATCACAT CACTCTTCCA AGTTTTGCCT 
CAACAAGTGA GCATTGAAGC CATCCAGGAA GTGGAAATGT TGCCAAGGCC ATCAAAAGTT 
ACTAGAGAAG AACATTTTCA ATTACATCAT CAAATGCACT ATTCTCAACA GCATTTGGAA 
ACTCCTGAAG ACAAGTCATT TGGCCGGTCA TTGACAGAGA CCGAAGCAAA CCTGGACAGT 
TATCAAACAG CTTTGGAAGA AGTACTCTCG TGGCTTCTTT CAGCTGAGGA TGCACTGCAA 
GCCCAAGGAG AGATTTCTAA TGATGTCGAA GAAGTGAAAG AACAATTTCA TACTCATGAG 
GGATATATGA TGGACTTGAC ATCCCATCAG GGACGGGTCG GTAATGTTCT CCAACTGGGA 
AGTCAACTGA TTGGAACAGG GAAATTATCA GAAGATGAAG AAACCGAAGT GCAGGAACAA 
ATGAATCTCC TCAATTCAAG ATGGGAATGC CTCAGGGTAG CTAGCATGGA AAAACAAAGC 
AATTTACATA AAGTTCTAAT GGATCTCCAG AATCAGCAAC TGAAAGAGTT AAATGACTGG 
CTAACCAAAA CAGAAGAGAG AACAAGGAAA ATGGAGAAGG AGCCCCTTGG ACCTGATATT 
GAAGACCTAA AACGCCAAGT ACAACAACAT AAGGTGCTTC AAGAAGACTT AGAACAGGAA 
CAAGTCAGGG TCAATTCCCT CACTCATATG GTGGTGGTAG TCGATGAATC TAGTGGAGAC 
CATGCAACTG CTGCTTTGGA AGAACAACTT AAGGTACTGG GAGATCGATG GGCAAACATC 
TGTAGGTGGA CAGAAGATCG CTGGGTTCTT TTACAAGACA TCCTCCTAAA ATGGCAGCGT 
TTTACTGAAG AACAGTGCCT TTTCAGTGCA TGGCTTTCGG AGAAGGAAGA TGCAGTGAAC 
AAGATTCACA CAACTGGCTT TAAGGATCAA AGTGAAGTGT TATCAAATCT TCAGAAACTG 
GCTGTCTTAA AAACAGATCT GGAAAAGAAG AAGCAAACCA TGGACAAACT CTGCTCACTC 
AACCAAGACC TTCTTTCAGC GCTGAAAAAC ACAGTGGTAG CCCACAAGAT GGAAGCATGG 
CTGGACAACT TTGCCCAGCG CTGGGATAAT TTAGTCCAGA AACTTGAAAA AAGTTCAGCA 
CAGATTTCAC AGGCTGTCAC CACCACTCAG CCATCACTAA CACAGACAAC TGTAATGGAA 
ACAGTAACTA TGGTGACCAC GAGGGAACAC ATCTTGGTAA AGCATGCCCA AGAGGAACTG 
CCACCACCAC CCCCTCAGAA GAAGAGGCAG ATTATCGTGG ATTCTGAAAT TAGGAAAAGG 
TTGGATGTCG ATATAACTGA ACTTCACAGT TGGATTACTC GTTCAGAAGC TGTGTTGCAG 
AGTCCTGAAT TTGCAATCTA TCGGAAGGAA GGCAACTTCT CAGACCTTAA AGAAAAAGTC 
AATGCCATAG AGCGAGAAAA AGCCGAGAAG TTCAGAAAAC TGCAAGATGC CAGCAGATCA 
GCTCAGGCCC TGGTGGAACA GATGGTGAAT GAGGGTGTTA ATGCTGACAG CATCAAACAA 
GCCTCCGAAC AACTGAACAG CCGGTGGATA GAGTTCTGCC AATTGCTAAG CGAGAGACTT 
AACTGGCTGG AGTATCAGAA CAACATCATC ACTTTCTATA ATCAGCTACA ACAATTGGAG 
CAGATGACAA CTACTGCTGA AAACTGGTTG AAAACCCAGC CTACCACCAC ATCAGAGCCA 
ACAGCAATTA AAAGCCAGTT AAAAATTTGT AAGGATGAAA TCAACCGACT GTCAGCTCTT 
CAGCCTCAAA TCGAGCGATT AAAAATTCAA AGCATAGCCC TGAAAGAGAA AGGACAAGGG 
CCAATGTTCC TGGATGCAGA CTTTGTGGCC TTTACAAATC ATTTTAACCA AGTCTTTGCT 
GATGTGCAGG CAAGAGAAAA AGAGCTACAA ACAATTTTTG ACAGTTTGCC ACCCATGCGC 
TATCAGGAGA CTATGAGTAC CATCCTGACA TGGATCCAGC AGTCAGAAAC CAAACTCTCT 
ATACCTCAGG TTACTGTCAC TGAATATGAC ATCATGGAAC AGAGACTCGG AGAGCTACAG 
GCTTTACAAA GCTCTCTGCA AGAGCAACAA AATGGCCTAA ACTATCTCAG CACCACTGTG 
AAAGAGATGT CAAAGAAAGC ACCACTGTCT GATATTAGTC GGAAATATCA ATCAGAATTT 
GAAGAGATTG AGGGACGTTG GAAGAAGCTG TCTTCCCAGC TGGTTGAACA TTGTCAAAAG 
TTGGAGGAGC AAATGGCTAA ACTTCGAAAA ATTCAGAATC ACATAAAAAC TCTGAAGAAA 
TGGATCACTG AAGTCGATGT TTTCCTGAAG GAGGAATGGC CTGCCCTTGG GGATTCAGAA 
ATTCTGAAAA GACAGCTGAA ACAGTGCAGG CTTTTAGTCA ATGACATTCA GACCATCCAG 
CCTAGTCTCA ACAGTGTCAA TGAAGGGGCT CAGAAGATGA AGAATGAAGC AGAACCAGAG 
TTTGCTGGCA GACTTGAGAC AGAGCTCCGA GAACTTAACA CCCAGTGGGA TTACATGTGC 
CGCCAGGTCT ATGCCAGGAA GGAAGCCTTA AAAGGAGGTT TGGATAAAAC TGTAAGTCTT 
CAGAAAGATC TGTCAGAGAT GCATGAGTGG ATGACACAAG CTGAAGAAGA ATACCTAGAG 
AGAGATTTCG AATACAAGAC CCCTGATGAA TTACAGACAG CAGTTGAAGA GATGAAGAGA 
GCTAAAGAAG AGGCCCAGCA AAAAGAAGCA AAAGTGAAAC TCCTAACCGA GTCCGTCAAT 
AGTGTCATAG CTCAGGCTCC ACCTGCAGCA CAAGAGGCCT TAAAAAAGGA ACTTGACACT 
CTCACCACCA ACTACCAGTG GCTCTGCACC AGGCTCAATG GCAAATGCAA GACCTTGGAA 
GAAGTTTGGG CGTGCTGGCA TGAGTTATTG TCCTACTTGG AGAAGGCAAA CAAGTGGCTA 
AGTGAAGTAG AAGTCAAGCT TAAAACCACT GAAAATATTT CTGGGGGAGC TGAGGAAATC 
GCCGAGGTGC TTGATTCGCT TGAAAATTTG ATGCAACATT CAGAGGATAA CCCGAATCAG 
ATTCGCATAT TGGCACAGAC CTTGACAGAT GGTGGAGTCA TGGATGAACT GATCAATGAG 
GAGCTTGAGA CATTTAATTC TCGTTGGAGA GAACTCCATG AAGAGGCTGT GAGGAGGCAA 
AAGTTGCTTG AGCAGAGTAT CCAGTCGGCC CAGGAGATAG AAAAATCCTT GCACTTAATT 
CAGGAGTCCC TCTCTTCCAT TGACAAGCAG TTGGCAGCTT ATATTGCTGA CAAAGTGGAT 
GCAGCTCAGA TGCCTCAGGA AGCCCAGAAA ATCCAATCAG ATTTGACAAG TCATGAGATC 
AGTTTAGAAG AAATGAAGAA ACATAACCAG GGAAAGGAGA CTGCCCAAAG GGTACTATCC 
CAAATTGATG TGGCACAGAA AAAATTGCAG GATGTTTCCA TGAAGTTTCG ATTATTCCAG 
AAGCCAGCCA ATTTTGAGCA GCGCCTACAA GAAAGTAAAA TGATTTTAGA TGAAGTGAAG 
ATGCATTTAC CTGCGTTGGA AACAAAGAGT GTGGAACAGG AAGTAGTACA GTCACAGTTA 
AATCATTGTG TGAACTTGTA TAAAAGTCTG AGTGAAGTGA AGTCTGAAGT GGAAATGGTA 
ATAAAAACTG GACGTCAGAT TGTACAGAAG AAGCAGACGG AAAACCCGAA AGAGCTTGAT 
GAAAGAGTTA CAGCTTTGAA ATTGCATTAT AATGAGCTGG GAGCAAAGGT GACAGAAAGA 
AAGCAACAGT TGGAAAAATG CTTGAAATTG TCCCGTAAGA TGCGAAAGGA AATGAATGCC 
CTGACAGAAT GGCTGGCAGC GACAGATATG GAACTGACGA AGAGATCGGC AGTTGAAGGA 
ATGCCTAGTA ATTTGGATTC TGAAGTTGCC TGGGGAAAGG CTACTCAGAA AGAGATTGAG 
AAACAGAAGG TTCACCTAAA GAGTGTCACA GAGGTAGGAG AGGCCTTGAA AACGGTTTTG 
GGCAAGAAGG AAATGTTGGT GGAAGATAAA CTGAGTCTTC TGAATAGTAA CTGGATAGCC 
GTCACTTCCC GAGCAGAAGA GTGGTTAAAC CTTTTATTGG AATACCAGAA ACACATGGAA 
AACTTTGACC AGAATGTGGA TTACATCACA AACTGGATCA TTCAGGCTGA TGCACTTTTG 
GATGAATCTG AGAAAAAGAA ACCTCAGCAA AAAGAAGACA TACTTAAGCG TTTAAAGGCT 
GAAATGAATG ACATACGTCC AAAGGTGGAT TCTACACGTG ACCAAGCAGC AAACCTGATG 
GCAAACCGCG GCGACCACTG CAGGAAAGTA GTAGAGCCCA AAATCTCAGA GCTCAACCAT 
CGATTTGCAG CCATTTCTCA CAGAATTAAG ACTGGAAAGG CCTCCATTCC TTTGAAGGAA 
TTGGAGCAGT TTAACTCAGA TATACAAAAA TTGCTTGAAC CACTGGAGGC TGAAATTCAG 
CAGGGGGTGA ATCTGAAAGA GGAAGACTTC AATAAAGATA TGAGTGAAGA CAATGAGGGT 
ACTGTAAAAG AATTGTTGCA AAGAGGAGAC AACTTACAAC AAAGAATCAC AGATGAGAGA 
AAGCGAGAGG AAATAAAGAT AAAACAACAG CTGTTACAGA CAAAACATAA TGCTCTCAAG 
GATTTGAGGT CTCAAAGAAG AAAAAAGGCT CTAGAAATTT CTCACCAGTG GTATCAGTAC 
AAGAGGCAGG CTGATGATCT CCTGAAATGC TTGGATGACA TTGAAAAAAA ATTAGCCAGC 
CTACCTGAAC CCAGAGATGA AAGGAAAATA AAGGAAATTG ATCGTGAATT GCAGAAGAAG 
AAAGAGGAGC TGAATGCAGT GCGTAGGCAA GCTGAGGGCT TGTCTGAGGA TGGGGCCGCA 
ATGGCAGTGG AGCCAACTCA GATCCAGCTC AGCAAGCGCT GGCGGGAAAT TGAGAGCAAA 
TTTGCTCAGT TTCGAAGACT CAACTTTGCA CAAATTCACA CTGTCCATGA AGAGTCAGTG 
GTGGCGATGA CTGAAGACAT GCCTTTGGAA ATTTCTTATG TGCCTTCTAC TTACCTGACT 
GAGATCACTC ATGTCTCACA AGCCCTATCA GAAGTGGAAG AGCTTCTTAA TGCTCCCGAC 
CTTTGTGCTC AAGATTTTGA AGATCTCTTT AAACAAGAGG AATCCTTGAA GAACATAAAA 
GACAGCCTGC AACAAATCTC AGGTCGGATT GACATCATTC ACAATAAAAA GACAGCAGCA 
TTGCACAGTG CCACTCCTGC AGAAAGGGCA AAGCTCCAGG AAGCTCTCTC ACGGCTTGAT 
TTCCAATGGG AAAGAGTTAA CAATATGTAC AAGGACCGAC AAGGGAGATT TGACAGATCT 
GTGGAAAAAT GGCGGCGGTT TCATTATGAT ATGAAGATAC TTAATCAATG GCTAACAGAA 
GCTGAACAGT TTCTCAAAAA GACACAAATT CCTGAGAATT GGGAACATGC CAAATACAAA 
TGGTATCTTA AGGAACTCCA GGATGGCATT GGACAGCGGC AAAGTGTTGT CAGGGTATTG 
AATGCAACTG GGGAAGAAAT AATTCAACAG TCCTCAAAAA CAGATGCCAG TATTCTCCAA 
GAAAAACTGG GAAGCCTGAA TCTGCGGTGG CAGGAGGTCT GCAAACAGCT GGCAGAAAGA 
AAAAAGAGGC TAGAGGAACA GAAGAATATC TTGTCAGAAT TTCAAAGAGA TGTAAATGAA 
TTTGTTTTAT GGTTGGAAGA AGCGGATAAC GTTGCTAATA TTCCACTTGA ACCTGGAAAT 
GAGCAGCAGC TAAAAGAAAA ACTTGAACAA GTCAAGTTAC TGGCAGAAGA GTTGCCCCTG 
CGCCAGGGAA TTCTAAAACA ATTAAATGAA ACTGGAGGAA CAGTGCTTGT AAGTGCTCCC 
CTAAGCCCAG AAGAGCAAGA TAAACTTGAA AATAAGCTCA AGCAGACAAA TCTTCAGTGG 
ATAAAGGTTT CTAGAAATCT GCCTGAGAAG CAAGAAGAAA TTGAGGCACA CGTAAAAGAC 
CTTGGACAGC TGGAAGAGCA GTTAAATCAT CTGCTTCTAT GGCTGTCTCC TATTAGGAAT 
CAGTTGGAAA TTTACAATCA GCCAAATCAA ACAGGACCAT TTGACATCAA GGAAATTGAA 
GTAGCAGTTC AAGCTAAACA GCCGGATGTG GAAGGGATTT TGTCTAAAGG GCAGCATTTG 
TACAAGGAAA AACCAGCCAC TCAGCCAGCG AAGAGAAAGC TGGAAGATCT CAGCTCTGAT 
TGGAAGGTGG TAACTCAGTT GCTTCAAGAG CTGCGGGCAA AGCAGCCTGG CCCAGCTCCT 
GGACTGACCA CTGTCAGAGC CCCTCCCAGT CAGACTGTTA CTCTGGTGAC ACAACCCGCG 
GTTACCAAGG AAACTGCCAT CTCCAAACTA GAAATGCCAT CTTCATTGCT GTTGGAGGTA 
CCTGCACTGG CAGATTTCAA CCGAGCTTGG ACAGAACTTA CCGACTGGCT GTCTCTGCTT 
GATCGAGTTA TAAAATCACA GAGGGTGATG GTGGGTGATC TTGAAGACAT TAACGAGATG 
ATCATCAAGC AGAAGGCAAC GCTGCAGGAT TTGGAACAGA GGCGCCCCCA GTTGGAAGAA 
CTCATTACCG CTGCCCAGAA TTTGAAAAAC AAGACCAGCA ATCAAGAGGC TAGAACAATC 
ATTACTGATC GAATTGAAAG AATTCAGAGT CAGTGGGATG AAGTACAGGA ACATCTTCAG 
AACCGGAGGC AACAGTTGAA TGAAATGTTA AAGGATTCCA CACAATGGCT GGAAGCTAAA 
GAGGAGGCTG AGCAGGTGTT GGGGCAGGCC AGAGCCAAGC TTGAGTCATG GAAGGAGGCT 
CCCTACACAG TAGATGCAAT CCAAAAGAAA ATCACAGAAA CCAAGCAGTT GGCCAAAGAC 
CTCCGCCAGT GGCAGATAAA TGTAGATGTT GCAAATGATT TGGCACTGAA ACTTCTCCGA 
GATTATTCTG CAGATGATAC CAGAAAAGTA CACATGATAA CAGAGAACAT CAATGCCTCT 
TGGGCAAGCA TCCATAAAAG ATTGAGTGAG CGAGAGGCTG CTCTGGAAGA AACCCACAGA 
TTACTGCAAC AGTTCCCCTT GGACCTGGAG AAGTTCCTTG CCTGGCTTAC AGAAGCCGAA 
ACAACTGCCA ACGTCCTGCA GGATGCCACC CATAAGGAAA GGCTTCTAGA AGATTCCAAG 
GGAGTAAGAG AGCTGATGAA ACAATGGCAA GATCTCCAAG GAGAAATCGA AGCTCACACA 
GATATCTATC ACAACCTGGA CGAAAATGGC CAAAAAGTCC TGAGATCCCT GGAAGGTTCT 
GACGATGCAG CCTTGTTGCA AAGACGTTTG GATAACATGA ACTTCAAGTG GAGCGAACTT 
CGGAAAAAGT CTCTCAACAT TAGGTCTCAC TTGGAAGCCA GTTCTGACCA GTGGAAGCGT 
CTGCACCTTT CTCTTCAGGA ACTTCTGGTA TGGCTCCAGC TGAAAGATGA TGAGTTAAGC 
CGGCAGGCAC CCATTGGAGG AGACTTTCCA GCGGTGCAGA AGCAGAATGA TGTACACAGG 
GCCTTCAAGA GGGAATTGAA AACGAAAGAA CCTGTAATCA TGAGTACTCT TGAGACTGTA 
CGAATATTTC TGACAGAGCA GCCTTTAGAA GGACTAGAGA AACTCTACCA GGAGCCCAGA 
GAGCTGCCTC CTGAAGAGAG AGCCCAGAAT GTCACACGGC TCCTACGAAA GCAAGCTGAG 
GAGGTCAACA CTCAGTGGGA AAAACTGAAC GTGCACTCTG CAGACTGGCA GAGAAAAATA 
GACGAGGCCC TCGAAAGACT CCAGGAGCTT CAGGAAGCAA CAGATGAGCT GGATCTCAAA 
CTACGTCAGG CAGAGGTGAT CAAAGGATCC TGGCAGCCTG TGGGTGATCT CCTCATTGAC 
TCTCTCCAAG ATCACCTCGA AAAAGTCAAG GCGCTTCGAG GAGAAATTAC ACCTCTGAAA 
GAGAATGTCA GCTACGTCAA TGACCTTGCT CGCCAACTCA CTACGTTGGG CATTCAGCTG 
TCACCATATA ACCTCAACAC TCTGGAAGAC CTGAACACCA GATGGAAGCT TCTGCAGGTG 
GCCATTGAGG ACCGCATCAG GCAGCTGCAT GAAGCGCACA GGGACTTTGG ACCAGCCTCC 
CAGCACTTCC TTTCCACTTC TGTCCAGGGT CCCTGGGAGA GAGCCATCTC ACCAAACAAA 
GTGCCCTACT ATATCAACCA CGAGACCCAA ACAACTTGCT GGGACCATCC CAAAATGACA 
GAGCTCTACC AGTCTTTAGC TGACCTGAAT AATGTCAGGT TCTCAGCTTA CAGGACTGCC 
ATGAAACTCC GAAGACTGCA GAAGGCCCTT TGCTTGGATC TCTTGAGCCT ATCGGCTGCA 
TGCGATGCCT TGGACCAGCA CAACCTCAAG CAAAATGACC AGCCCATGGA TATCCTGCAG 
GTCATTAACT GTCTGACCAC TATTTATGAT CGCCTAGAGC AAGAGCACAA CAATCTGGTC 
AACGTCCCTC TCTGCGTGGA TATGTGTCTC AATTGGCTGC TGAATGTTTA TGACACGGGA 
CGAACGGGGA GGATCCGGGT CCTGTCTTTT AAAACTGGCA TCATTTCTCT GTGTAAAGCC 
CATTTGGAAG ACAAGTACAG ATACCTCTTC AAGCAAGTGG CAAGTTCGAC AGGATTTTGT 
GACCAGCGCA GGCTGGGCCT CCTCCTGCAT GACTCTATCC AGATCCCAAG ACAGTTGGGT 
GAAGTCGCAT CGTTCGGGGG CAGTAACATT GAGCCGAGTG TCAGGAGCTG CTTCCAGTTT 
GCTAATAATA AGCCTGAGAT CGAAGCGGCC CTCTTCCTAG ACTGGATGCG CCTGGAGCCC 
CAGTCCATGG TGTGGCTGCC TGTCCTGCAC CGAGTGGCTG CCGCGGAAAC TGCCAAGCAC 
CAGGCCAAGT GCAACATCTG CAAGGAGTGT CCCATCATCG GATTCAGGTA CAGGAGTCTA 
AAGCACTTTA ATTATGACAT CTGCCAAAGT TGCTTTTTTT CTGGTCGAGT TGCAAAAGGC 
CATAAAATGC ACTATCCCAT GGTGGAATAC TGCACTCCGA CTACATCGGG AGAAGATGTC 
CGTGACTTTG CCAAGGTACT AAAAAACAAA TTTCGAACCA AAAGGTATTT TGCGAAGCAT 
CCCCGAATGG GCTACCTGCC AGTGCAGACT GTCTTAGAGG GGGACAACAT GGAAACTCCT 
GTCACTCTGA TCAACTTCTG GCCGGTAGAT TCTGCGCCTG CCTCGTCCCC TCAGCTTTCA 
CACGATGATA CTCATTCACG CATTGAGCAT TATGCTAGCA GGCTAGCAGA AATGGAAAAC 
AGCAATGGAT CTTATCTAAA TGATAGCATC TCTCCTAATG AGAGCATAGA TGATGAACAT 
TTGTTAATCC AGCATTACTG CCAAAGTTTG AACCAGGACT CCCCCCTGAG CCAGCCTCGT 
AGTCCTGCCC AGATCTTGAT TTCCTTAGAG AGTGAGGAAA GAGGGGAGCT AGAGAGAATC 
CTAGCAGATC TTGAGGAAGA AAACAGAAAT CTGCAAGCAG AGTATGATCG TCTAAAGCAG 
CAGCATGAAC ACAAAGGCCT GTCCCCACTG CCATCCCCTC CTGAAATGAT GCCTACTTCT 
CCCCAAAGTC CCCGGGATGC TGAGCTCATC GCTGAGGCCA AGCTGCTGCG TCAACACAAA 
GGCCGCCTGG AAGCCAGGAT GCAAATCCTT GAAGACCATA ACAAACAACT GGAATCCCAG 
TTACACAGGC TCAGGCAGCT GCTGGAGCAA CCCCAGGCAG AGGCCAAGGT GAATGGTACA 
ACGGTGTCTT CTCCTTCTAC CTCTCTTCAG AGGTCAGATA GCAGTCAGCC TATGCTGCTC 
CGGGTGGTCG GCAGTCAGAC TTCAGAATCC ATGGGCGAGG AAGACCTGCT CAGCCCTCCC 
CAGGACACAA GCACAGGGTT AGAGGAAGTG ATGGAGCAGC TCAACCACTC CTTCCCTAGT 
TCCAGAGGAC ACAATGTAGG AAGCCTTTTC CACTTGGCAG ATGATTTGGG CAGAGCGATG 
GAGTCCTTAG TTTCAGTCAT GACAGATGAA GAAGGGGCAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013966822.1
CDS	248..11212
Translation	MLWWEEVEDCYEREDVQKKTFTKWINAQFSKFGKQHIENLFSDLQDGRRLLDLLEGLTGQKLPKEKGSTRVHALNNVNKALRVLQKNNVDLVNIGSTDIVDGNHKLTLGLIWNIILHWQVKNVMKNIMAGLQQTNSEKILLSWVRQSTRNYPQVNVINFTTSWSDGLALNALIHSHRPDLFDWNSVVCQQSATQRLEHAFNIAKYQLGIEKLLDPEDVATTYPDKKSILMYITSLFQVLPQQVSIEAIQEVEMLPRPSKVTREEHFQLHHQMHYSQQHLETPEDKSFGRSLTETEANLDSYQTALEEVLSWLLSAEDALQAQGEISNDVEEVKEQFHTHEGYMMDLTSHQGRVGNVLQLGSQLIGTGKLSEDEETEVQEQMNLLNSRWECLRVASMEKQSNLHKVLMDLQNQQLKELNDWLTKTEERTRKMEKEPLGPDIEDLKRQVQQHKVLQEDLEQEQVRVNSLTHMVVVVDESSGDHATAALEEQLKVLGDRWANICRWTEDRWVLLQDILLKWQRFTEEQCLFSAWLSEKEDAVNKIHTTGFKDQSEVLSNLQKLAVLKTDLEKKKQTMDKLCSLNQDLLSALKNTVVAHKMEAWLDNFAQRWDNLVQKLEKSSAQISQAVTTTQPSLTQTTVMETVTMVTTREHILVKHAQEELPPPPPQKKRQIIVDSEIRKRLDVDITELHSWITRSEAVLQSPEFAIYRKEGNFSDLKEKVNAIEREKAEKFRKLQDASRSAQALVEQMVNEGVNADSIKQASEQLNSRWIEFCQLLSERLNWLEYQNNIITFYNQLQQLEQMTTTAENWLKTQPTTTSEPTAIKSQLKICKDEINRLSALQPQIERLKIQSIALKEKGQGPMFLDADFVAFTNHFNQVFADVQAREKELQTIFDSLPPMRYQETMSTILTWIQQSETKLSIPQVTVTEYDIMEQRLGELQALQSSLQEQQNGLNYLSTTVKEMSKKAPLSDISRKYQSEFEEIEGRWKKLSSQLVEHCQKLEEQMAKLRKIQNHIKTLKKWITEVDVFLKEEWPALGDSEILKRQLKQCRLLVNDIQTIQPSLNSVNEGAQKMKNEAEPEFAGRLETELRELNTQWDYMCRQVYARKEALKGGLDKTVSLQKDLSEMHEWMTQAEEEYLERDFEYKTPDELQTAVEEMKRAKEEAQQKEAKVKLLTESVNSVIAQAPPAAQEALKKELDTLTTNYQWLCTRLNGKCKTLEEVWACWHELLSYLEKANKWLSEVEVKLKTTENISGGAEEIAEVLDSLENLMQHSEDNPNQIRILAQTLTDGGVMDELINEELETFNSRWRELHEEAVRRQKLLEQSIQSAQEIEKSLHLIQESLSSIDKQLAAYIADKVDAAQMPQEAQKIQSDLTSHEISLEEMKKHNQGKETAQRVLSQIDVAQKKLQDVSMKFRLFQKPANFEQRLQESKMILDEVKMHLPALETKSVEQEVVQSQLNHCVNLYKSLSEVKSEVEMVIKTGRQIVQKKQTENPKELDERVTALKLHYNELGAKVTERKQQLEKCLKLSRKMRKEMNALTEWLAATDMELTKRSAVEGMPSNLDSEVAWGKATQKEIEKQKVHLKSVTEVGEALKTVLGKKEMLVEDKLSLLNSNWIAVTSRAEEWLNLLLEYQKHMENFDQNVDYITNWIIQADALLDESEKKKPQQKEDILKRLKAEMNDIRPKVDSTRDQAANLMANRGDHCRKVVEPKISELNHRFAAISHRIKTGKASIPLKELEQFNSDIQKLLEPLEAEIQQGVNLKEEDFNKDMSEDNEGTVKELLQRGDNLQQRITDERKREEIKIKQQLLQTKHNALKDLRSQRRKKALEISHQWYQYKRQADDLLKCLDDIEKKLASLPEPRDERKIKEIDRELQKKKEELNAVRRQAEGLSEDGAAMAVEPTQIQLSKRWREIESKFAQFRRLNFAQIHTVHEESVVAMTEDMPLEISYVPSTYLTEITHVSQALSEVEELLNAPDLCAQDFEDLFKQEESLKNIKDSLQQISGRIDIIHNKKTAALHSATPAERAKLQEALSRLDFQWERVNNMYKDRQGRFDRSVEKWRRFHYDMKILNQWLTEAEQFLKKTQIPENWEHAKYKWYLKELQDGIGQRQSVVRVLNATGEEIIQQSSKTDASILQEKLGSLNLRWQEVCKQLAERKKRLEEQKNILSEFQRDVNEFVLWLEEADNVANIPLEPGNEQQLKEKLEQVKLLAEELPLRQGILKQLNETGGTVLVSAPLSPEEQDKLENKLKQTNLQWIKVSRNLPEKQEEIEAHVKDLGQLEEQLNHLLLWLSPIRNQLEIYNQPNQTGPFDIKEIEVAVQAKQPDVEGILSKGQHLYKEKPATQPAKRKLEDLSSDWKVVTQLLQELRAKQPGPAPGLTTVRAPPSQTVTLVTQPAVTKETAISKLEMPSSLLLEVPALADFNRAWTELTDWLSLLDRVIKSQRVMVGDLEDINEMIIKQKATLQDLEQRRPQLEELITAAQNLKNKTSNQEARTIITDRIERIQSQWDEVQEHLQNRRQQLNEMLKDSTQWLEAKEEAEQVLGQARAKLESWKEAPYTVDAIQKKITETKQLAKDLRQWQINVDVANDLALKLLRDYSADDTRKVHMITENINASWASIHKRLSEREAALEETHRLLQQFPLDLEKFLAWLTEAETTANVLQDATHKERLLEDSKGVRELMKQWQDLQGEIEAHTDIYHNLDENGQKVLRSLEGSDDAALLQRRLDNMNFKWSELRKKSLNIRSHLEASSDQWKRLHLSLQELLVWLQLKDDELSRQAPIGGDFPAVQKQNDVHRAFKRELKTKEPVIMSTLETVRIFLTEQPLEGLEKLYQEPRELPPEERAQNVTRLLRKQAEEVNTQWEKLNVHSADWQRKIDEALERLQELQEATDELDLKLRQAEVIKGSWQPVGDLLIDSLQDHLEKVKALRGEITPLKENVSYVNDLARQLTTLGIQLSPYNLNTLEDLNTRWKLLQVAIEDRIRQLHEAHRDFGPASQHFLSTSVQGPWERAISPNKVPYYINHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQVINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPVTLINFWPVDSAPASSPQLSHDDTHSRIEHYASRLAEMENSNGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKQQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDTSTGLEEVMEQLNHSFPSSRGHNVGSLFHLADDLGRAMESLVSVMTDEEGAE

Target ORF information:

RefSeq Version	XM_014111347.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris dystrophin (DMD), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111347.1

ORF Insert Sequence:

ATGCTTTGGT GGGAAGAAGT AGAGGACTGT TATGAAAGAG AAGATGTTCA AAAGAAAACA 
TTCACAAAAT GGATAAATGC ACAGTTTTCT AAGTTTGGGA AGCAGCACAT AGAGAACCTC 
TTCAGTGACC TACAGGATGG GAGACGCCTC CTAGACCTTT TGGAAGGCCT GACAGGGCAA 
AAACTGCCAA AAGAAAAAGG ATCCACAAGA GTTCATGCCC TGAACAATGT CAACAAGGCA 
CTGCGCGTCT TGCAGAAAAA TAATGTTGAT TTAGTGAACA TTGGAAGTAC TGACATAGTA 
GATGGAAATC ACAAACTGAC TCTTGGTTTG ATTTGGAATA TAATCCTCCA CTGGCAGGTC 
AAAAATGTAA TGAAAAATAT CATGGCTGGA TTGCAACAAA CCAACAGTGA AAAGATTCTC 
CTGAGCTGGG TCCGACAATC AACTCGTAAT TATCCACAGG TTAATGTCAT TAACTTCACC 
ACCAGCTGGT CTGATGGCCT GGCTTTGAAC GCTCTCATCC ACAGTCATAG GCCAGACCTG 
TTTGATTGGA ATAGTGTGGT TTGCCAGCAG TCAGCCACAC AACGCCTGGA ACATGCATTC 
AACATTGCCA AATATCAATT AGGCATAGAG AAACTGCTTG ATCCTGAAGA TGTTGCCACC 
ACTTATCCAG ATAAGAAGTC CATCTTAATG TATATCACAT CACTCTTCCA AGTTTTGCCT 
CAACAAGTGA GCATTGAAGC CATCCAGGAA GTGGAAATGT TGCCAAGGCC ATCAAAAGTT 
ACTAGAGAAG AACATTTTCA ATTACATCAT CAAATGCACT ATTCTCAACA GCATTTGGAA 
ACTCCTGAAG ACAAGTCATT TGGCCGGTCA TTGACAGAGA CCGAAGCAAA CCTGGACAGT 
TATCAAACAG CTTTGGAAGA AGTACTCTCG TGGCTTCTTT CAGCTGAGGA TGCACTGCAA 
GCCCAAGGAG AGATTTCTAA TGATGTCGAA GAAGTGAAAG AACAATTTCA TACTCATGAG 
GGATATATGA TGGACTTGAC ATCCCATCAG GGACGGGTCG GTAATGTTCT CCAACTGGGA 
AGTCAACTGA TTGGAACAGG GAAATTATCA GAAGATGAAG AAACCGAAGT GCAGGAACAA 
ATGAATCTCC TCAATTCAAG ATGGGAATGC CTCAGGGTAG CTAGCATGGA AAAACAAAGC 
AATTTACATA AAGTTCTAAT GGATCTCCAG AATCAGCAAC TGAAAGAGTT AAATGACTGG 
CTAACCAAAA CAGAAGAGAG AACAAGGAAA ATGGAGAAGG AGCCCCTTGG ACCTGATATT 
GAAGACCTAA AACGCCAAGT ACAACAACAT AAGGTGCTTC AAGAAGACTT AGAACAGGAA 
CAAGTCAGGG TCAATTCCCT CACTCATATG GTGGTGGTAG TCGATGAATC TAGTGGAGAC 
CATGCAACTG CTGCTTTGGA AGAACAACTT AAGGTACTGG GAGATCGATG GGCAAACATC 
TGTAGGTGGA CAGAAGATCG CTGGGTTCTT TTACAAGACA TCCTCCTAAA ATGGCAGCGT 
TTTACTGAAG AACAGTGCCT TTTCAGTGCA TGGCTTTCGG AGAAGGAAGA TGCAGTGAAC 
AAGATTCACA CAACTGGCTT TAAGGATCAA AGTGAAGTGT TATCAAATCT TCAGAAACTG 
GCTGTCTTAA AAACAGATCT GGAAAAGAAG AAGCAAACCA TGGACAAACT CTGCTCACTC 
AACCAAGACC TTCTTTCAGC GCTGAAAAAC ACAGTGGTAG CCCACAAGAT GGAAGCATGG 
CTGGACAACT TTGCCCAGCG CTGGGATAAT TTAGTCCAGA AACTTGAAAA AAGTTCAGCA 
CAGATTTCAC AGGCTGTCAC CACCACTCAG CCATCACTAA CACAGACAAC TGTAATGGAA 
ACAGTAACTA TGGTGACCAC GAGGGAACAC ATCTTGGTAA AGCATGCCCA AGAGGAACTG 
CCACCACCAC CCCCTCAGAA GAAGAGGCAG ATTATCGTGG ATTCTGAAAT TAGGAAAAGG 
TTGGATGTCG ATATAACTGA ACTTCACAGT TGGATTACTC GTTCAGAAGC TGTGTTGCAG 
AGTCCTGAAT TTGCAATCTA TCGGAAGGAA GGCAACTTCT CAGACCTTAA AGAAAAAGTC 
AATGCCATAG AGCGAGAAAA AGCCGAGAAG TTCAGAAAAC TGCAAGATGC CAGCAGATCA 
GCTCAGGCCC TGGTGGAACA GATGGTGAAT GAGGGTGTTA ATGCTGACAG CATCAAACAA 
GCCTCCGAAC AACTGAACAG CCGGTGGATA GAGTTCTGCC AATTGCTAAG CGAGAGACTT 
AACTGGCTGG AGTATCAGAA CAACATCATC ACTTTCTATA ATCAGCTACA ACAATTGGAG 
CAGATGACAA CTACTGCTGA AAACTGGTTG AAAACCCAGC CTACCACCAC ATCAGAGCCA 
ACAGCAATTA AAAGCCAGTT AAAAATTTGT AAGGATGAAA TCAACCGACT GTCAGCTCTT 
CAGCCTCAAA TCGAGCGATT AAAAATTCAA AGCATAGCCC TGAAAGAGAA AGGACAAGGG 
CCAATGTTCC TGGATGCAGA CTTTGTGGCC TTTACAAATC ATTTTAACCA AGTCTTTGCT 
GATGTGCAGG CAAGAGAAAA AGAGCTACAA ACAATTTTTG ACAGTTTGCC ACCCATGCGC 
TATCAGGAGA CTATGAGTAC CATCCTGACA TGGATCCAGC AGTCAGAAAC CAAACTCTCT 
ATACCTCAGG TTACTGTCAC TGAATATGAC ATCATGGAAC AGAGACTCGG AGAGCTACAG 
GCTTTACAAA GCTCTCTGCA AGAGCAACAA AATGGCCTAA ACTATCTCAG CACCACTGTG 
AAAGAGATGT CAAAGAAAGC ACCACTGTCT GATATTAGTC GGAAATATCA ATCAGAATTT 
GAAGAGATTG AGGGACGTTG GAAGAAGCTG TCTTCCCAGC TGGTTGAACA TTGTCAAAAG 
TTGGAGGAGC AAATGGCTAA ACTTCGAAAA ATTCAGAATC ACATAAAAAC TCTGAAGAAA 
TGGATCACTG AAGTCGATGT TTTCCTGAAG GAGGAATGGC CTGCCCTTGG GGATTCAGAA 
ATTCTGAAAA GACAGCTGAA ACAGTGCAGG CTTTTAGTCA ATGACATTCA GACCATCCAG 
CCTAGTCTCA ACAGTGTCAA TGAAGGGGCT CAGAAGATGA AGAATGAAGC AGAACCAGAG 
TTTGCTGGCA GACTTGAGAC AGAGCTCCGA GAACTTAACA CCCAGTGGGA TTACATGTGC 
CGCCAGGTCT ATGCCAGGAA GGAAGCCTTA AAAGGAGGTT TGGATAAAAC TGTAAGTCTT 
CAGAAAGATC TGTCAGAGAT GCATGAGTGG ATGACACAAG CTGAAGAAGA ATACCTAGAG 
AGAGATTTCG AATACAAGAC CCCTGATGAA TTACAGACAG CAGTTGAAGA GATGAAGAGA 
GCTAAAGAAG AGGCCCAGCA AAAAGAAGCA AAAGTGAAAC TCCTAACCGA GTCCGTCAAT 
AGTGTCATAG CTCAGGCTCC ACCTGCAGCA CAAGAGGCCT TAAAAAAGGA ACTTGACACT 
CTCACCACCA ACTACCAGTG GCTCTGCACC AGGCTCAATG GCAAATGCAA GACCTTGGAA 
GAAGTTTGGG CGTGCTGGCA TGAGTTATTG TCCTACTTGG AGAAGGCAAA CAAGTGGCTA 
AGTGAAGTAG AAGTCAAGCT TAAAACCACT GAAAATATTT CTGGGGGAGC TGAGGAAATC 
GCCGAGGTGC TTGATTCGCT TGAAAATTTG ATGCAACATT CAGAGGATAA CCCGAATCAG 
ATTCGCATAT TGGCACAGAC CTTGACAGAT GGTGGAGTCA TGGATGAACT GATCAATGAG 
GAGCTTGAGA CATTTAATTC TCGTTGGAGA GAACTCCATG AAGAGGCTGT GAGGAGGCAA 
AAGTTGCTTG AGCAGAGTAT CCAGTCGGCC CAGGAGATAG AAAAATCCTT GCACTTAATT 
CAGGAGTCCC TCTCTTCCAT TGACAAGCAG TTGGCAGCTT ATATTGCTGA CAAAGTGGAT 
GCAGCTCAGA TGCCTCAGGA AGCCCAGAAA ATCCAATCAG ATTTGACAAG TCATGAGATC 
AGTTTAGAAG AAATGAAGAA ACATAACCAG GGAAAGGAGA CTGCCCAAAG GGTACTATCC 
CAAATTGATG TGGCACAGAA AAAATTGCAG GATGTTTCCA TGAAGTTTCG ATTATTCCAG 
AAGCCAGCCA ATTTTGAGCA GCGCCTACAA GAAAGTAAAA TGATTTTAGA TGAAGTGAAG 
ATGCATTTAC CTGCGTTGGA AACAAAGAGT GTGGAACAGG AAGTAGTACA GTCACAGTTA 
AATCATTGTG TGAACTTGTA TAAAAGTCTG AGTGAAGTGA AGTCTGAAGT GGAAATGGTA 
ATAAAAACTG GACGTCAGAT TGTACAGAAG AAGCAGACGG AAAACCCGAA AGAGCTTGAT 
GAAAGAGTTA CAGCTTTGAA ATTGCATTAT AATGAGCTGG GAGCAAAGGT GACAGAAAGA 
AAGCAACAGT TGGAAAAATG CTTGAAATTG TCCCGTAAGA TGCGAAAGGA AATGAATGCC 
CTGACAGAAT GGCTGGCAGC GACAGATATG GAACTGACGA AGAGATCGGC AGTTGAAGGA 
ATGCCTAGTA ATTTGGATTC TGAAGTTGCC TGGGGAAAGG CTACTCAGAA AGAGATTGAG 
AAACAGAAGG TTCACCTAAA GAGTGTCACA GAGGTAGGAG AGGCCTTGAA AACGGTTTTG 
GGCAAGAAGG AAATGTTGGT GGAAGATAAA CTGAGTCTTC TGAATAGTAA CTGGATAGCC 
GTCACTTCCC GAGCAGAAGA GTGGTTAAAC CTTTTATTGG AATACCAGAA ACACATGGAA 
AACTTTGACC AGAATGTGGA TTACATCACA AACTGGATCA TTCAGGCTGA TGCACTTTTG 
GATGAATCTG AGAAAAAGAA ACCTCAGCAA AAAGAAGACA TACTTAAGCG TTTAAAGGCT 
GAAATGAATG ACATACGTCC AAAGGTGGAT TCTACACGTG ACCAAGCAGC AAACCTGATG 
GCAAACCGCG GCGACCACTG CAGGAAAGTA GTAGAGCCCA AAATCTCAGA GCTCAACCAT 
CGATTTGCAG CCATTTCTCA CAGAATTAAG ACTGGAAAGG CCTCCATTCC TTTGAAGGAA 
TTGGAGCAGT TTAACTCAGA TATACAAAAA TTGCTTGAAC CACTGGAGGC TGAAATTCAG 
CAGGGGGTGA ATCTGAAAGA GGAAGACTTC AATAAAGATA TGAGTGAAGA CAATGAGGGT 
ACTGTAAAAG AATTGTTGCA AAGAGGAGAC AACTTACAAC AAAGAATCAC AGATGAGAGA 
AAGCGAGAGG AAATAAAGAT AAAACAACAG CTGTTACAGA CAAAACATAA TGCTCTCAAG 
GATTTGAGGT CTCAAAGAAG AAAAAAGGCT CTAGAAATTT CTCACCAGTG GTATCAGTAC 
AAGAGGCAGG CTGATGATCT CCTGAAATGC TTGGATGACA TTGAAAAAAA ATTAGCCAGC 
CTACCTGAAC CCAGAGATGA AAGGAAAATA AAGGAAATTG ATCGTGAATT GCAGAAGAAG 
AAAGAGGAGC TGAATGCAGT GCGTAGGCAA GCTGAGGGCT TGTCTGAGGA TGGGGCCGCA 
ATGGCAGTGG AGCCAACTCA GATCCAGCTC AGCAAGCGCT GGCGGGAAAT TGAGAGCAAA 
TTTGCTCAGT TTCGAAGACT CAACTTTGCA CAAATTCACA CTGTCCATGA AGAGTCAGTG 
GTGGCGATGA CTGAAGACAT GCCTTTGGAA ATTTCTTATG TGCCTTCTAC TTACCTGACT 
GAGATCACTC ATGTCTCACA AGCCCTATCA GAAGTGGAAG AGCTTCTTAA TGCTCCCGAC 
CTTTGTGCTC AAGATTTTGA AGATCTCTTT AAACAAGAGG AATCCTTGAA GAACATAAAA 
GACAGCCTGC AACAAATCTC AGGTCGGATT GACATCATTC ACAATAAAAA GACAGCAGCA 
TTGCACAGTG CCACTCCTGC AGAAAGGGCA AAGCTCCAGG AAGCTCTCTC ACGGCTTGAT 
TTCCAATGGG AAAGAGTTAA CAATATGTAC AAGGACCGAC AAGGGAGATT TGACAGATCT 
GTGGAAAAAT GGCGGCGGTT TCATTATGAT ATGAAGATAC TTAATCAATG GCTAACAGAA 
GCTGAACAGT TTCTCAAAAA GACACAAATT CCTGAGAATT GGGAACATGC CAAATACAAA 
TGGTATCTTA AGGAACTCCA GGATGGCATT GGACAGCGGC AAAGTGTTGT CAGGGTATTG 
AATGCAACTG GGGAAGAAAT AATTCAACAG TCCTCAAAAA CAGATGCCAG TATTCTCCAA 
GAAAAACTGG GAAGCCTGAA TCTGCGGTGG CAGGAGGTCT GCAAACAGCT GGCAGAAAGA 
AAAAAGAGGC TAGAGGAACA GAAGAATATC TTGTCAGAAT TTCAAAGAGA TGTAAATGAA 
TTTGTTTTAT GGTTGGAAGA AGCGGATAAC GTTGCTAATA TTCCACTTGA ACCTGGAAAT 
GAGCAGCAGC TAAAAGAAAA ACTTGAACAA GTCAAGTTAC TGGCAGAAGA GTTGCCCCTG 
CGCCAGGGAA TTCTAAAACA ATTAAATGAA ACTGGAGGAA CAGTGCTTGT AAGTGCTCCC 
CTAAGCCCAG AAGAGCAAGA TAAACTTGAA AATAAGCTCA AGCAGACAAA TCTTCAGTGG 
ATAAAGGTTT CTAGAAATCT GCCTGAGAAG CAAGAAGAAA TTGAGGCACA CGTAAAAGAC 
CTTGGACAGC TGGAAGAGCA GTTAAATCAT CTGCTTCTAT GGCTGTCTCC TATTAGGAAT 
CAGTTGGAAA TTTACAATCA GCCAAATCAA ACAGGACCAT TTGACATCAA GGAAATTGAA 
GTAGCAGTTC AAGCTAAACA GCCGGATGTG GAAGGGATTT TGTCTAAAGG GCAGCATTTG 
TACAAGGAAA AACCAGCCAC TCAGCCAGCG AAGAGAAAGC TGGAAGATCT CAGCTCTGAT 
TGGAAGGTGG TAACTCAGTT GCTTCAAGAG CTGCGGGCAA AGCAGCCTGG CCCAGCTCCT 
GGACTGACCA CTGTCAGAGC CCCTCCCAGT CAGACTGTTA CTCTGGTGAC ACAACCCGCG 
GTTACCAAGG AAACTGCCAT CTCCAAACTA GAAATGCCAT CTTCATTGCT GTTGGAGGTA 
CCTGCACTGG CAGATTTCAA CCGAGCTTGG ACAGAACTTA CCGACTGGCT GTCTCTGCTT 
GATCGAGTTA TAAAATCACA GAGGGTGATG GTGGGTGATC TTGAAGACAT TAACGAGATG 
ATCATCAAGC AGAAGGCAAC GCTGCAGGAT TTGGAACAGA GGCGCCCCCA GTTGGAAGAA 
CTCATTACCG CTGCCCAGAA TTTGAAAAAC AAGACCAGCA ATCAAGAGGC TAGAACAATC 
ATTACTGATC GAATTGAAAG AATTCAGAGT CAGTGGGATG AAGTACAGGA ACATCTTCAG 
AACCGGAGGC AACAGTTGAA TGAAATGTTA AAGGATTCCA CACAATGGCT GGAAGCTAAA 
GAGGAGGCTG AGCAGGTGTT GGGGCAGGCC AGAGCCAAGC TTGAGTCATG GAAGGAGGCT 
CCCTACACAG TAGATGCAAT CCAAAAGAAA ATCACAGAAA CCAAGCAGTT GGCCAAAGAC 
CTCCGCCAGT GGCAGATAAA TGTAGATGTT GCAAATGATT TGGCACTGAA ACTTCTCCGA 
GATTATTCTG CAGATGATAC CAGAAAAGTA CACATGATAA CAGAGAACAT CAATGCCTCT 
TGGGCAAGCA TCCATAAAAG ATTGAGTGAG CGAGAGGCTG CTCTGGAAGA AACCCACAGA 
TTACTGCAAC AGTTCCCCTT GGACCTGGAG AAGTTCCTTG CCTGGCTTAC AGAAGCCGAA 
ACAACTGCCA ACGTCCTGCA GGATGCCACC CATAAGGAAA GGCTTCTAGA AGATTCCAAG 
GGAGTAAGAG AGCTGATGAA ACAATGGCAA GATCTCCAAG GAGAAATCGA AGCTCACACA 
GATATCTATC ACAACCTGGA CGAAAATGGC CAAAAAGTCC TGAGATCCCT GGAAGGTTCT 
GACGATGCAG CCTTGTTGCA AAGACGTTTG GATAACATGA ACTTCAAGTG GAGCGAACTT 
CGGAAAAAGT CTCTCAACAT TAGGTCTCAC TTGGAAGCCA GTTCTGACCA GTGGAAGCGT 
CTGCACCTTT CTCTTCAGGA ACTTCTGGTA TGGCTCCAGC TGAAAGATGA TGAGTTAAGC 
CGGCAGGCAC CCATTGGAGG AGACTTTCCA GCGGTGCAGA AGCAGAATGA TGTACACAGG 
GCCTTCAAGA GGGAATTGAA AACGAAAGAA CCTGTAATCA TGAGTACTCT TGAGACTGTA 
CGAATATTTC TGACAGAGCA GCCTTTAGAA GGACTAGAGA AACTCTACCA GGAGCCCAGA 
GAGCTGCCTC CTGAAGAGAG AGCCCAGAAT GTCACACGGC TCCTACGAAA GCAAGCTGAG 
GAGGTCAACA CTCAGTGGGA AAAACTGAAC GTGCACTCTG CAGACTGGCA GAGAAAAATA 
GACGAGGCCC TCGAAAGACT CCAGGAGCTT CAGGAAGCAA CAGATGAGCT GGATCTCAAA 
CTACGTCAGG CAGAGGTGAT CAAAGGATCC TGGCAGCCTG TGGGTGATCT CCTCATTGAC 
TCTCTCCAAG ATCACCTCGA AAAAGTCAAG GCGCTTCGAG GAGAAATTAC ACCTCTGAAA 
GAGAATGTCA GCTACGTCAA TGACCTTGCT CGCCAACTCA CTACGTTGGG CATTCAGCTG 
TCACCATATA ACCTCAACAC TCTGGAAGAC CTGAACACCA GATGGAAGCT TCTGCAGGTG 
GCCATTGAGG ACCGCATCAG GCAGCTGCAT GAAGCGCACA GGGACTTTGG ACCAGCCTCC 
CAGCACTTCC TTTCCACTTC TGTCCAGGGT CCCTGGGAGA GAGCCATCTC ACCAAACAAA 
GTGCCCTACT ATATCAACCA CGAGACCCAA ACAACTTGCT GGGACCATCC CAAAATGACA 
GAGCTCTACC AGTCTTTAGC TGACCTGAAT AATGTCAGGT TCTCAGCTTA CAGGACTGCC 
ATGAAACTCC GAAGACTGCA GAAGGCCCTT TGCTTGGATC TCTTGAGCCT ATCGGCTGCA 
TGCGATGCCT TGGACCAGCA CAACCTCAAG CAAAATGACC AGCCCATGGA TATCCTGCAG 
GTCATTAACT GTCTGACCAC TATTTATGAT CGCCTAGAGC AAGAGCACAA CAATCTGGTC 
AACGTCCCTC TCTGCGTGGA TATGTGTCTC AATTGGCTGC TGAATGTTTA TGACACGGGA 
CGAACGGGGA GGATCCGGGT CCTGTCTTTT AAAACTGGCA TCATTTCTCT GTGTAAAGCC 
CATTTGGAAG ACAAGTACAG ATACCTCTTC AAGCAAGTGG CAAGTTCGAC AGGATTTTGT 
GACCAGCGCA GGCTGGGCCT CCTCCTGCAT GACTCTATCC AGATCCCAAG ACAGTTGGGT 
GAAGTCGCAT CGTTCGGGGG CAGTAACATT GAGCCGAGTG TCAGGAGCTG CTTCCAGTTT 
GCTAATAATA AGCCTGAGAT CGAAGCGGCC CTCTTCCTAG ACTGGATGCG CCTGGAGCCC 
CAGTCCATGG TGTGGCTGCC TGTCCTGCAC CGAGTGGCTG CCGCGGAAAC TGCCAAGCAC 
CAGGCCAAGT GCAACATCTG CAAGGAGTGT CCCATCATCG GATTCAGGTA CAGGAGTCTA 
AAGCACTTTA ATTATGACAT CTGCCAAAGT TGCTTTTTTT CTGGTCGAGT TGCAAAAGGC 
CATAAAATGC ACTATCCCAT GGTGGAATAC TGCACTCCGA CTACATCGGG AGAAGATGTC 
CGTGACTTTG CCAAGGTACT AAAAAACAAA TTTCGAACCA AAAGGTATTT TGCGAAGCAT 
CCCCGAATGG GCTACCTGCC AGTGCAGACT GTCTTAGAGG GGGACAACAT GGAAACTCCT 
GTCACTCTGA TCAACTTCTG GCCGGTAGAT TCTGCGCCTG CCTCGTCCCC TCAGCTTTCA 
CACGATGATA CTCATTCACG CATTGAGCAT TATGCTAGCA GGCTAGCAGA AATGGAAAAC 
AGCAATGGAT CTTATCTAAA TGATAGCATC TCTCCTAATG AGAGCATAGA TGATGAACAT 
TTGTTAATCC AGCATTACTG CCAAAGTTTG AACCAGGACT CCCCCCTGAG CCAGCCTCGT 
AGTCCTGCCC AGATCTTGAT TTCCTTAGAG AGTGAGGAAA GAGGGGAGCT AGAGAGAATC 
CTAGCAGATC TTGAGGAAGA AAACAGAAAT CTGCAAGCAG AGTATGATCG TCTAAAGCAG 
CAGCATGAAC ACAAAGGCCT GTCCCCACTG CCATCCCCTC CTGAAATGAT GCCTACTTCT 
CCCCAAAGTC CCCGGGATGC TGAGCTCATC GCTGAGGCCA AGCTGCTGCG TCAACACAAA 
GGCCGCCTGG AAGCCAGGAT GCAAATCCTT GAAGACCATA ACAAACAACT GGAATCCCAG 
TTACACAGGC TCAGGCAGCT GCTGGAGCAA CCCCAGGCAG AGGCCAAGGT GAATGGTACA 
ACGGTGTCTT CTCCTTCTAC CTCTCTTCAG AGGTCAGATA GCAGTCAGCC TATGCTGCTC 
CGGGTGGTCG GCAGTCAGAC TTCAGAATCC ATGGGCGAGG AAGACCTGCT CAGCCCTCCC 
CAGGACACAA GCACAGGGTT AGAGGAAGTG ATGGAGCAGC TCAACCACTC CTTCCCTAGT 
TCCAGAGGAC ACAATGTAGG AAGCCTTTTC CACTTGGCAG ATGATTTGGG CAGAGCGATG 
GAGTCCTTAG TTTCAGTCAT GACAGATGAA GAAGGGGCAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMD ( XM_014111347.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013966822.1 Canis lupus familiaris dystrophin (DMD), transcript variant X8, mRNA.

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CloneID	OCe45418
Clone ID Related Accession (Same CDS sequence)	XM_014111347.1 , XM_014111347.2
Accession Version	XM_014111347.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	dystrophin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014111347.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTGGT GGGAAGAAGT AGAGGACTGT TATGAAAGAG AAGATGTTCA AAAGAAAACA 
TTCACAAAAT GGATAAATGC ACAGTTTTCT AAGTTTGGGA AGCAGCACAT AGAGAACCTC 
TTCAGTGACC TACAGGATGG GAGACGCCTC CTAGACCTTT TGGAAGGCCT GACAGGGCAA 
AAACTGCCAA AAGAAAAAGG ATCCACAAGA GTTCATGCCC TGAACAATGT CAACAAGGCA 
CTGCGCGTCT TGCAGAAAAA TAATGTTGAT TTAGTGAACA TTGGAAGTAC TGACATAGTA 
GATGGAAATC ACAAACTGAC TCTTGGTTTG ATTTGGAATA TAATCCTCCA CTGGCAGGTC 
AAAAATGTAA TGAAAAATAT CATGGCTGGA TTGCAACAAA CCAACAGTGA AAAGATTCTC 
CTGAGCTGGG TCCGACAATC AACTCGTAAT TATCCACAGG TTAATGTCAT TAACTTCACC 
ACCAGCTGGT CTGATGGCCT GGCTTTGAAC GCTCTCATCC ACAGTCATAG GCCAGACCTG 
TTTGATTGGA ATAGTGTGGT TTGCCAGCAG TCAGCCACAC AACGCCTGGA ACATGCATTC 
AACATTGCCA AATATCAATT AGGCATAGAG AAACTGCTTG ATCCTGAAGA TGTTGCCACC 
ACTTATCCAG ATAAGAAGTC CATCTTAATG TATATCACAT CACTCTTCCA AGTTTTGCCT 
CAACAAGTGA GCATTGAAGC CATCCAGGAA GTGGAAATGT TGCCAAGGCC ATCAAAAGTT 
ACTAGAGAAG AACATTTTCA ATTACATCAT CAAATGCACT ATTCTCAACA GCATTTGGAA 
ACTCCTGAAG ACAAGTCATT TGGCCGGTCA TTGACAGAGA CCGAAGCAAA CCTGGACAGT 
TATCAAACAG CTTTGGAAGA AGTACTCTCG TGGCTTCTTT CAGCTGAGGA TGCACTGCAA 
GCCCAAGGAG AGATTTCTAA TGATGTCGAA GAAGTGAAAG AACAATTTCA TACTCATGAG 
GGATATATGA TGGACTTGAC ATCCCATCAG GGACGGGTCG GTAATGTTCT CCAACTGGGA 
AGTCAACTGA TTGGAACAGG GAAATTATCA GAAGATGAAG AAACCGAAGT GCAGGAACAA 
ATGAATCTCC TCAATTCAAG ATGGGAATGC CTCAGGGTAG CTAGCATGGA AAAACAAAGC 
AATTTACATA AAGTTCTAAT GGATCTCCAG AATCAGCAAC TGAAAGAGTT AAATGACTGG 
CTAACCAAAA CAGAAGAGAG AACAAGGAAA ATGGAGAAGG AGCCCCTTGG ACCTGATATT 
GAAGACCTAA AACGCCAAGT ACAACAACAT AAGGTGCTTC AAGAAGACTT AGAACAGGAA 
CAAGTCAGGG TCAATTCCCT CACTCATATG GTGGTGGTAG TCGATGAATC TAGTGGAGAC 
CATGCAACTG CTGCTTTGGA AGAACAACTT AAGGTACTGG GAGATCGATG GGCAAACATC 
TGTAGGTGGA CAGAAGATCG CTGGGTTCTT TTACAAGACA TCCTCCTAAA ATGGCAGCGT 
TTTACTGAAG AACAGTGCCT TTTCAGTGCA TGGCTTTCGG AGAAGGAAGA TGCAGTGAAC 
AAGATTCACA CAACTGGCTT TAAGGATCAA AGTGAAGTGT TATCAAATCT TCAGAAACTG 
GCTGTCTTAA AAACAGATCT GGAAAAGAAG AAGCAAACCA TGGACAAACT CTGCTCACTC 
AACCAAGACC TTCTTTCAGC GCTGAAAAAC ACAGTGGTAG CCCACAAGAT GGAAGCATGG 
CTGGACAACT TTGCCCAGCG CTGGGATAAT TTAGTCCAGA AACTTGAAAA AAGTTCAGCA 
CAGATTTCAC AGGCTGTCAC CACCACTCAG CCATCACTAA CACAGACAAC TGTAATGGAA 
ACAGTAACTA TGGTGACCAC GAGGGAACAC ATCTTGGTAA AGCATGCCCA AGAGGAACTG 
CCACCACCAC CCCCTCAGAA GAAGAGGCAG ATTATCGTGG ATTCTGAAAT TAGGAAAAGG 
TTGGATGTCG ATATAACTGA ACTTCACAGT TGGATTACTC GTTCAGAAGC TGTGTTGCAG 
AGTCCTGAAT TTGCAATCTA TCGGAAGGAA GGCAACTTCT CAGACCTTAA AGAAAAAGTC 
AATGCCATAG AGCGAGAAAA AGCCGAGAAG TTCAGAAAAC TGCAAGATGC CAGCAGATCA 
GCTCAGGCCC TGGTGGAACA GATGGTGAAT GAGGGTGTTA ATGCTGACAG CATCAAACAA 
GCCTCCGAAC AACTGAACAG CCGGTGGATA GAGTTCTGCC AATTGCTAAG CGAGAGACTT 
AACTGGCTGG AGTATCAGAA CAACATCATC ACTTTCTATA ATCAGCTACA ACAATTGGAG 
CAGATGACAA CTACTGCTGA AAACTGGTTG AAAACCCAGC CTACCACCAC ATCAGAGCCA 
ACAGCAATTA AAAGCCAGTT AAAAATTTGT AAGGATGAAA TCAACCGACT GTCAGCTCTT 
CAGCCTCAAA TCGAGCGATT AAAAATTCAA AGCATAGCCC TGAAAGAGAA AGGACAAGGG 
CCAATGTTCC TGGATGCAGA CTTTGTGGCC TTTACAAATC ATTTTAACCA AGTCTTTGCT 
GATGTGCAGG CAAGAGAAAA AGAGCTACAA ACAATTTTTG ACAGTTTGCC ACCCATGCGC 
TATCAGGAGA CTATGAGTAC CATCCTGACA TGGATCCAGC AGTCAGAAAC CAAACTCTCT 
ATACCTCAGG TTACTGTCAC TGAATATGAC ATCATGGAAC AGAGACTCGG AGAGCTACAG 
GCTTTACAAA GCTCTCTGCA AGAGCAACAA AATGGCCTAA ACTATCTCAG CACCACTGTG 
AAAGAGATGT CAAAGAAAGC ACCACTGTCT GATATTAGTC GGAAATATCA ATCAGAATTT 
GAAGAGATTG AGGGACGTTG GAAGAAGCTG TCTTCCCAGC TGGTTGAACA TTGTCAAAAG 
TTGGAGGAGC AAATGGCTAA ACTTCGAAAA ATTCAGAATC ACATAAAAAC TCTGAAGAAA 
TGGATCACTG AAGTCGATGT TTTCCTGAAG GAGGAATGGC CTGCCCTTGG GGATTCAGAA 
ATTCTGAAAA GACAGCTGAA ACAGTGCAGG CTTTTAGTCA ATGACATTCA GACCATCCAG 
CCTAGTCTCA ACAGTGTCAA TGAAGGGGCT CAGAAGATGA AGAATGAAGC AGAACCAGAG 
TTTGCTGGCA GACTTGAGAC AGAGCTCCGA GAACTTAACA CCCAGTGGGA TTACATGTGC 
CGCCAGGTCT ATGCCAGGAA GGAAGCCTTA AAAGGAGGTT TGGATAAAAC TGTAAGTCTT 
CAGAAAGATC TGTCAGAGAT GCATGAGTGG ATGACACAAG CTGAAGAAGA ATACCTAGAG 
AGAGATTTCG AATACAAGAC CCCTGATGAA TTACAGACAG CAGTTGAAGA GATGAAGAGA 
GCTAAAGAAG AGGCCCAGCA AAAAGAAGCA AAAGTGAAAC TCCTAACCGA GTCCGTCAAT 
AGTGTCATAG CTCAGGCTCC ACCTGCAGCA CAAGAGGCCT TAAAAAAGGA ACTTGACACT 
CTCACCACCA ACTACCAGTG GCTCTGCACC AGGCTCAATG GCAAATGCAA GACCTTGGAA 
GAAGTTTGGG CGTGCTGGCA TGAGTTATTG TCCTACTTGG AGAAGGCAAA CAAGTGGCTA 
AGTGAAGTAG AAGTCAAGCT TAAAACCACT GAAAATATTT CTGGGGGAGC TGAGGAAATC 
GCCGAGGTGC TTGATTCGCT TGAAAATTTG ATGCAACATT CAGAGGATAA CCCGAATCAG 
ATTCGCATAT TGGCACAGAC CTTGACAGAT GGTGGAGTCA TGGATGAACT GATCAATGAG 
GAGCTTGAGA CATTTAATTC TCGTTGGAGA GAACTCCATG AAGAGGCTGT GAGGAGGCAA 
AAGTTGCTTG AGCAGAGTAT CCAGTCGGCC CAGGAGATAG AAAAATCCTT GCACTTAATT 
CAGGAGTCCC TCTCTTCCAT TGACAAGCAG TTGGCAGCTT ATATTGCTGA CAAAGTGGAT 
GCAGCTCAGA TGCCTCAGGA AGCCCAGAAA ATCCAATCAG ATTTGACAAG TCATGAGATC 
AGTTTAGAAG AAATGAAGAA ACATAACCAG GGAAAGGAGA CTGCCCAAAG GGTACTATCC 
CAAATTGATG TGGCACAGAA AAAATTGCAG GATGTTTCCA TGAAGTTTCG ATTATTCCAG 
AAGCCAGCCA ATTTTGAGCA GCGCCTACAA GAAAGTAAAA TGATTTTAGA TGAAGTGAAG 
ATGCATTTAC CTGCGTTGGA AACAAAGAGT GTGGAACAGG AAGTAGTACA GTCACAGTTA 
AATCATTGTG TGAACTTGTA TAAAAGTCTG AGTGAAGTGA AGTCTGAAGT GGAAATGGTA 
ATAAAAACTG GACGTCAGAT TGTACAGAAG AAGCAGACGG AAAACCCGAA AGAGCTTGAT 
GAAAGAGTTA CAGCTTTGAA ATTGCATTAT AATGAGCTGG GAGCAAAGGT GACAGAAAGA 
AAGCAACAGT TGGAAAAATG CTTGAAATTG TCCCGTAAGA TGCGAAAGGA AATGAATGCC 
CTGACAGAAT GGCTGGCAGC GACAGATATG GAACTGACGA AGAGATCGGC AGTTGAAGGA 
ATGCCTAGTA ATTTGGATTC TGAAGTTGCC TGGGGAAAGG CTACTCAGAA AGAGATTGAG 
AAACAGAAGG TTCACCTAAA GAGTGTCACA GAGGTAGGAG AGGCCTTGAA AACGGTTTTG 
GGCAAGAAGG AAATGTTGGT GGAAGATAAA CTGAGTCTTC TGAATAGTAA CTGGATAGCC 
GTCACTTCCC GAGCAGAAGA GTGGTTAAAC CTTTTATTGG AATACCAGAA ACACATGGAA 
AACTTTGACC AGAATGTGGA TTACATCACA AACTGGATCA TTCAGGCTGA TGCACTTTTG 
GATGAATCTG AGAAAAAGAA ACCTCAGCAA AAAGAAGACA TACTTAAGCG TTTAAAGGCT 
GAAATGAATG ACATACGTCC AAAGGTGGAT TCTACACGTG ACCAAGCAGC AAACCTGATG 
GCAAACCGCG GCGACCACTG CAGGAAAGTA GTAGAGCCCA AAATCTCAGA GCTCAACCAT 
CGATTTGCAG CCATTTCTCA CAGAATTAAG ACTGGAAAGG CCTCCATTCC TTTGAAGGAA 
TTGGAGCAGT TTAACTCAGA TATACAAAAA TTGCTTGAAC CACTGGAGGC TGAAATTCAG 
CAGGGGGTGA ATCTGAAAGA GGAAGACTTC AATAAAGATA TGAGTGAAGA CAATGAGGGT 
ACTGTAAAAG AATTGTTGCA AAGAGGAGAC AACTTACAAC AAAGAATCAC AGATGAGAGA 
AAGCGAGAGG AAATAAAGAT AAAACAACAG CTGTTACAGA CAAAACATAA TGCTCTCAAG 
GATTTGAGGT CTCAAAGAAG AAAAAAGGCT CTAGAAATTT CTCACCAGTG GTATCAGTAC 
AAGAGGCAGG CTGATGATCT CCTGAAATGC TTGGATGACA TTGAAAAAAA ATTAGCCAGC 
CTACCTGAAC CCAGAGATGA AAGGAAAATA AAGGAAATTG ATCGTGAATT GCAGAAGAAG 
AAAGAGGAGC TGAATGCAGT GCGTAGGCAA GCTGAGGGCT TGTCTGAGGA TGGGGCCGCA 
ATGGCAGTGG AGCCAACTCA GATCCAGCTC AGCAAGCGCT GGCGGGAAAT TGAGAGCAAA 
TTTGCTCAGT TTCGAAGACT CAACTTTGCA CAAATTCACA CTGTCCATGA AGAGTCAGTG 
GTGGCGATGA CTGAAGACAT GCCTTTGGAA ATTTCTTATG TGCCTTCTAC TTACCTGACT 
GAGATCACTC ATGTCTCACA AGCCCTATCA GAAGTGGAAG AGCTTCTTAA TGCTCCCGAC 
CTTTGTGCTC AAGATTTTGA AGATCTCTTT AAACAAGAGG AATCCTTGAA GAACATAAAA 
GACAGCCTGC AACAAATCTC AGGTCGGATT GACATCATTC ACAATAAAAA GACAGCAGCA 
TTGCACAGTG CCACTCCTGC AGAAAGGGCA AAGCTCCAGG AAGCTCTCTC ACGGCTTGAT 
TTCCAATGGG AAAGAGTTAA CAATATGTAC AAGGACCGAC AAGGGAGATT TGACAGATCT 
GTGGAAAAAT GGCGGCGGTT TCATTATGAT ATGAAGATAC TTAATCAATG GCTAACAGAA 
GCTGAACAGT TTCTCAAAAA GACACAAATT CCTGAGAATT GGGAACATGC CAAATACAAA 
TGGTATCTTA AGGAACTCCA GGATGGCATT GGACAGCGGC AAAGTGTTGT CAGGGTATTG 
AATGCAACTG GGGAAGAAAT AATTCAACAG TCCTCAAAAA CAGATGCCAG TATTCTCCAA 
GAAAAACTGG GAAGCCTGAA TCTGCGGTGG CAGGAGGTCT GCAAACAGCT GGCAGAAAGA 
AAAAAGAGGC TAGAGGAACA GAAGAATATC TTGTCAGAAT TTCAAAGAGA TGTAAATGAA 
TTTGTTTTAT GGTTGGAAGA AGCGGATAAC GTTGCTAATA TTCCACTTGA ACCTGGAAAT 
GAGCAGCAGC TAAAAGAAAA ACTTGAACAA GTCAAGTTAC TGGCAGAAGA GTTGCCCCTG 
CGCCAGGGAA TTCTAAAACA ATTAAATGAA ACTGGAGGAA CAGTGCTTGT AAGTGCTCCC 
CTAAGCCCAG AAGAGCAAGA TAAACTTGAA AATAAGCTCA AGCAGACAAA TCTTCAGTGG 
ATAAAGGTTT CTAGAAATCT GCCTGAGAAG CAAGAAGAAA TTGAGGCACA CGTAAAAGAC 
CTTGGACAGC TGGAAGAGCA GTTAAATCAT CTGCTTCTAT GGCTGTCTCC TATTAGGAAT 
CAGTTGGAAA TTTACAATCA GCCAAATCAA ACAGGACCAT TTGACATCAA GGAAATTGAA 
GTAGCAGTTC AAGCTAAACA GCCGGATGTG GAAGGGATTT TGTCTAAAGG GCAGCATTTG 
TACAAGGAAA AACCAGCCAC TCAGCCAGCG AAGAGAAAGC TGGAAGATCT CAGCTCTGAT 
TGGAAGGTGG TAACTCAGTT GCTTCAAGAG CTGCGGGCAA AGCAGCCTGG CCCAGCTCCT 
GGACTGACCA CTGTCAGAGC CCCTCCCAGT CAGACTGTTA CTCTGGTGAC ACAACCCGCG 
GTTACCAAGG AAACTGCCAT CTCCAAACTA GAAATGCCAT CTTCATTGCT GTTGGAGGTA 
CCTGCACTGG CAGATTTCAA CCGAGCTTGG ACAGAACTTA CCGACTGGCT GTCTCTGCTT 
GATCGAGTTA TAAAATCACA GAGGGTGATG GTGGGTGATC TTGAAGACAT TAACGAGATG 
ATCATCAAGC AGAAGGCAAC GCTGCAGGAT TTGGAACAGA GGCGCCCCCA GTTGGAAGAA 
CTCATTACCG CTGCCCAGAA TTTGAAAAAC AAGACCAGCA ATCAAGAGGC TAGAACAATC 
ATTACTGATC GAATTGAAAG AATTCAGAGT CAGTGGGATG AAGTACAGGA ACATCTTCAG 
AACCGGAGGC AACAGTTGAA TGAAATGTTA AAGGATTCCA CACAATGGCT GGAAGCTAAA 
GAGGAGGCTG AGCAGGTGTT GGGGCAGGCC AGAGCCAAGC TTGAGTCATG GAAGGAGGCT 
CCCTACACAG TAGATGCAAT CCAAAAGAAA ATCACAGAAA CCAAGCAGTT GGCCAAAGAC 
CTCCGCCAGT GGCAGATAAA TGTAGATGTT GCAAATGATT TGGCACTGAA ACTTCTCCGA 
GATTATTCTG CAGATGATAC CAGAAAAGTA CACATGATAA CAGAGAACAT CAATGCCTCT 
TGGGCAAGCA TCCATAAAAG ATTGAGTGAG CGAGAGGCTG CTCTGGAAGA AACCCACAGA 
TTACTGCAAC AGTTCCCCTT GGACCTGGAG AAGTTCCTTG CCTGGCTTAC AGAAGCCGAA 
ACAACTGCCA ACGTCCTGCA GGATGCCACC CATAAGGAAA GGCTTCTAGA AGATTCCAAG 
GGAGTAAGAG AGCTGATGAA ACAATGGCAA GATCTCCAAG GAGAAATCGA AGCTCACACA 
GATATCTATC ACAACCTGGA CGAAAATGGC CAAAAAGTCC TGAGATCCCT GGAAGGTTCT 
GACGATGCAG CCTTGTTGCA AAGACGTTTG GATAACATGA ACTTCAAGTG GAGCGAACTT 
CGGAAAAAGT CTCTCAACAT TAGGTCTCAC TTGGAAGCCA GTTCTGACCA GTGGAAGCGT 
CTGCACCTTT CTCTTCAGGA ACTTCTGGTA TGGCTCCAGC TGAAAGATGA TGAGTTAAGC 
CGGCAGGCAC CCATTGGAGG AGACTTTCCA GCGGTGCAGA AGCAGAATGA TGTACACAGG 
GCCTTCAAGA GGGAATTGAA AACGAAAGAA CCTGTAATCA TGAGTACTCT TGAGACTGTA 
CGAATATTTC TGACAGAGCA GCCTTTAGAA GGACTAGAGA AACTCTACCA GGAGCCCAGA 
GAGCTGCCTC CTGAAGAGAG AGCCCAGAAT GTCACACGGC TCCTACGAAA GCAAGCTGAG 
GAGGTCAACA CTCAGTGGGA AAAACTGAAC GTGCACTCTG CAGACTGGCA GAGAAAAATA 
GACGAGGCCC TCGAAAGACT CCAGGAGCTT CAGGAAGCAA CAGATGAGCT GGATCTCAAA 
CTACGTCAGG CAGAGGTGAT CAAAGGATCC TGGCAGCCTG TGGGTGATCT CCTCATTGAC 
TCTCTCCAAG ATCACCTCGA AAAAGTCAAG GCGCTTCGAG GAGAAATTAC ACCTCTGAAA 
GAGAATGTCA GCTACGTCAA TGACCTTGCT CGCCAACTCA CTACGTTGGG CATTCAGCTG 
TCACCATATA ACCTCAACAC TCTGGAAGAC CTGAACACCA GATGGAAGCT TCTGCAGGTG 
GCCATTGAGG ACCGCATCAG GCAGCTGCAT GAAGCGCACA GGGACTTTGG ACCAGCCTCC 
CAGCACTTCC TTTCCACTTC TGTCCAGGGT CCCTGGGAGA GAGCCATCTC ACCAAACAAA 
GTGCCCTACT ATATCAACCA CGAGACCCAA ACAACTTGCT GGGACCATCC CAAAATGACA 
GAGCTCTACC AGTCTTTAGC TGACCTGAAT AATGTCAGGT TCTCAGCTTA CAGGACTGCC 
ATGAAACTCC GAAGACTGCA GAAGGCCCTT TGCTTGGATC TCTTGAGCCT ATCGGCTGCA 
TGCGATGCCT TGGACCAGCA CAACCTCAAG CAAAATGACC AGCCCATGGA TATCCTGCAG 
GTCATTAACT GTCTGACCAC TATTTATGAT CGCCTAGAGC AAGAGCACAA CAATCTGGTC 
AACGTCCCTC TCTGCGTGGA TATGTGTCTC AATTGGCTGC TGAATGTTTA TGACACGGGA 
CGAACGGGGA GGATCCGGGT CCTGTCTTTT AAAACTGGCA TCATTTCTCT GTGTAAAGCC 
CATTTGGAAG ACAAGTACAG ATACCTCTTC AAGCAAGTGG CAAGTTCGAC AGGATTTTGT 
GACCAGCGCA GGCTGGGCCT CCTCCTGCAT GACTCTATCC AGATCCCAAG ACAGTTGGGT 
GAAGTCGCAT CGTTCGGGGG CAGTAACATT GAGCCGAGTG TCAGGAGCTG CTTCCAGTTT 
GCTAATAATA AGCCTGAGAT CGAAGCGGCC CTCTTCCTAG ACTGGATGCG CCTGGAGCCC 
CAGTCCATGG TGTGGCTGCC TGTCCTGCAC CGAGTGGCTG CCGCGGAAAC TGCCAAGCAC 
CAGGCCAAGT GCAACATCTG CAAGGAGTGT CCCATCATCG GATTCAGGTA CAGGAGTCTA 
AAGCACTTTA ATTATGACAT CTGCCAAAGT TGCTTTTTTT CTGGTCGAGT TGCAAAAGGC 
CATAAAATGC ACTATCCCAT GGTGGAATAC TGCACTCCGA CTACATCGGG AGAAGATGTC 
CGTGACTTTG CCAAGGTACT AAAAAACAAA TTTCGAACCA AAAGGTATTT TGCGAAGCAT 
CCCCGAATGG GCTACCTGCC AGTGCAGACT GTCTTAGAGG GGGACAACAT GGAAACTCCT 
GTCACTCTGA TCAACTTCTG GCCGGTAGAT TCTGCGCCTG CCTCGTCCCC TCAGCTTTCA 
CACGATGATA CTCATTCACG CATTGAGCAT TATGCTAGCA GGCTAGCAGA AATGGAAAAC 
AGCAATGGAT CTTATCTAAA TGATAGCATC TCTCCTAATG AGAGCATAGA TGATGAACAT 
TTGTTAATCC AGCATTACTG CCAAAGTTTG AACCAGGACT CCCCCCTGAG CCAGCCTCGT 
AGTCCTGCCC AGATCTTGAT TTCCTTAGAG AGTGAGGAAA GAGGGGAGCT AGAGAGAATC 
CTAGCAGATC TTGAGGAAGA AAACAGAAAT CTGCAAGCAG AGTATGATCG TCTAAAGCAG 
CAGCATGAAC ACAAAGGCCT GTCCCCACTG CCATCCCCTC CTGAAATGAT GCCTACTTCT 
CCCCAAAGTC CCCGGGATGC TGAGCTCATC GCTGAGGCCA AGCTGCTGCG TCAACACAAA 
GGCCGCCTGG AAGCCAGGAT GCAAATCCTT GAAGACCATA ACAAACAACT GGAATCCCAG 
TTACACAGGC TCAGGCAGCT GCTGGAGCAA CCCCAGGCAG AGGCCAAGGT GAATGGTACA 
ACGGTGTCTT CTCCTTCTAC CTCTCTTCAG AGGTCAGATA GCAGTCAGCC TATGCTGCTC 
CGGGTGGTCG GCAGTCAGAC TTCAGAATCC ATGGGCGAGG AAGACCTGCT CAGCCCTCCC 
CAGGACACAA GCACAGGGTT AGAGGAAGTG ATGGAGCAGC TCAACCACTC CTTCCCTAGT 
TCCAGAGGAC ACAATGTAGG AAGCCTTTTC CACTTGGCAG ATGATTTGGG CAGAGCGATG 
GAGTCCTTAG TTTCAGTCAT GACAGATGAA GAAGGGGCAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013966822.1
CDS	247..11211
Translation	MLWWEEVEDCYEREDVQKKTFTKWINAQFSKFGKQHIENLFSDLQDGRRLLDLLEGLTGQKLPKEKGSTRVHALNNVNKALRVLQKNNVDLVNIGSTDIVDGNHKLTLGLIWNIILHWQVKNVMKNIMAGLQQTNSEKILLSWVRQSTRNYPQVNVINFTTSWSDGLALNALIHSHRPDLFDWNSVVCQQSATQRLEHAFNIAKYQLGIEKLLDPEDVATTYPDKKSILMYITSLFQVLPQQVSIEAIQEVEMLPRPSKVTREEHFQLHHQMHYSQQHLETPEDKSFGRSLTETEANLDSYQTALEEVLSWLLSAEDALQAQGEISNDVEEVKEQFHTHEGYMMDLTSHQGRVGNVLQLGSQLIGTGKLSEDEETEVQEQMNLLNSRWECLRVASMEKQSNLHKVLMDLQNQQLKELNDWLTKTEERTRKMEKEPLGPDIEDLKRQVQQHKVLQEDLEQEQVRVNSLTHMVVVVDESSGDHATAALEEQLKVLGDRWANICRWTEDRWVLLQDILLKWQRFTEEQCLFSAWLSEKEDAVNKIHTTGFKDQSEVLSNLQKLAVLKTDLEKKKQTMDKLCSLNQDLLSALKNTVVAHKMEAWLDNFAQRWDNLVQKLEKSSAQISQAVTTTQPSLTQTTVMETVTMVTTREHILVKHAQEELPPPPPQKKRQIIVDSEIRKRLDVDITELHSWITRSEAVLQSPEFAIYRKEGNFSDLKEKVNAIEREKAEKFRKLQDASRSAQALVEQMVNEGVNADSIKQASEQLNSRWIEFCQLLSERLNWLEYQNNIITFYNQLQQLEQMTTTAENWLKTQPTTTSEPTAIKSQLKICKDEINRLSALQPQIERLKIQSIALKEKGQGPMFLDADFVAFTNHFNQVFADVQAREKELQTIFDSLPPMRYQETMSTILTWIQQSETKLSIPQVTVTEYDIMEQRLGELQALQSSLQEQQNGLNYLSTTVKEMSKKAPLSDISRKYQSEFEEIEGRWKKLSSQLVEHCQKLEEQMAKLRKIQNHIKTLKKWITEVDVFLKEEWPALGDSEILKRQLKQCRLLVNDIQTIQPSLNSVNEGAQKMKNEAEPEFAGRLETELRELNTQWDYMCRQVYARKEALKGGLDKTVSLQKDLSEMHEWMTQAEEEYLERDFEYKTPDELQTAVEEMKRAKEEAQQKEAKVKLLTESVNSVIAQAPPAAQEALKKELDTLTTNYQWLCTRLNGKCKTLEEVWACWHELLSYLEKANKWLSEVEVKLKTTENISGGAEEIAEVLDSLENLMQHSEDNPNQIRILAQTLTDGGVMDELINEELETFNSRWRELHEEAVRRQKLLEQSIQSAQEIEKSLHLIQESLSSIDKQLAAYIADKVDAAQMPQEAQKIQSDLTSHEISLEEMKKHNQGKETAQRVLSQIDVAQKKLQDVSMKFRLFQKPANFEQRLQESKMILDEVKMHLPALETKSVEQEVVQSQLNHCVNLYKSLSEVKSEVEMVIKTGRQIVQKKQTENPKELDERVTALKLHYNELGAKVTERKQQLEKCLKLSRKMRKEMNALTEWLAATDMELTKRSAVEGMPSNLDSEVAWGKATQKEIEKQKVHLKSVTEVGEALKTVLGKKEMLVEDKLSLLNSNWIAVTSRAEEWLNLLLEYQKHMENFDQNVDYITNWIIQADALLDESEKKKPQQKEDILKRLKAEMNDIRPKVDSTRDQAANLMANRGDHCRKVVEPKISELNHRFAAISHRIKTGKASIPLKELEQFNSDIQKLLEPLEAEIQQGVNLKEEDFNKDMSEDNEGTVKELLQRGDNLQQRITDERKREEIKIKQQLLQTKHNALKDLRSQRRKKALEISHQWYQYKRQADDLLKCLDDIEKKLASLPEPRDERKIKEIDRELQKKKEELNAVRRQAEGLSEDGAAMAVEPTQIQLSKRWREIESKFAQFRRLNFAQIHTVHEESVVAMTEDMPLEISYVPSTYLTEITHVSQALSEVEELLNAPDLCAQDFEDLFKQEESLKNIKDSLQQISGRIDIIHNKKTAALHSATPAERAKLQEALSRLDFQWERVNNMYKDRQGRFDRSVEKWRRFHYDMKILNQWLTEAEQFLKKTQIPENWEHAKYKWYLKELQDGIGQRQSVVRVLNATGEEIIQQSSKTDASILQEKLGSLNLRWQEVCKQLAERKKRLEEQKNILSEFQRDVNEFVLWLEEADNVANIPLEPGNEQQLKEKLEQVKLLAEELPLRQGILKQLNETGGTVLVSAPLSPEEQDKLENKLKQTNLQWIKVSRNLPEKQEEIEAHVKDLGQLEEQLNHLLLWLSPIRNQLEIYNQPNQTGPFDIKEIEVAVQAKQPDVEGILSKGQHLYKEKPATQPAKRKLEDLSSDWKVVTQLLQELRAKQPGPAPGLTTVRAPPSQTVTLVTQPAVTKETAISKLEMPSSLLLEVPALADFNRAWTELTDWLSLLDRVIKSQRVMVGDLEDINEMIIKQKATLQDLEQRRPQLEELITAAQNLKNKTSNQEARTIITDRIERIQSQWDEVQEHLQNRRQQLNEMLKDSTQWLEAKEEAEQVLGQARAKLESWKEAPYTVDAIQKKITETKQLAKDLRQWQINVDVANDLALKLLRDYSADDTRKVHMITENINASWASIHKRLSEREAALEETHRLLQQFPLDLEKFLAWLTEAETTANVLQDATHKERLLEDSKGVRELMKQWQDLQGEIEAHTDIYHNLDENGQKVLRSLEGSDDAALLQRRLDNMNFKWSELRKKSLNIRSHLEASSDQWKRLHLSLQELLVWLQLKDDELSRQAPIGGDFPAVQKQNDVHRAFKRELKTKEPVIMSTLETVRIFLTEQPLEGLEKLYQEPRELPPEERAQNVTRLLRKQAEEVNTQWEKLNVHSADWQRKIDEALERLQELQEATDELDLKLRQAEVIKGSWQPVGDLLIDSLQDHLEKVKALRGEITPLKENVSYVNDLARQLTTLGIQLSPYNLNTLEDLNTRWKLLQVAIEDRIRQLHEAHRDFGPASQHFLSTSVQGPWERAISPNKVPYYINHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQVINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPVTLINFWPVDSAPASSPQLSHDDTHSRIEHYASRLAEMENSNGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKQQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDTSTGLEEVMEQLNHSFPSSRGHNVGSLFHLADDLGRAMESLVSVMTDEEGAE

Target ORF information:

RefSeq Version	XM_014111347.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris dystrophin (DMD), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111347.2

ORF Insert Sequence:

ATGCTTTGGT GGGAAGAAGT AGAGGACTGT TATGAAAGAG AAGATGTTCA AAAGAAAACA 
TTCACAAAAT GGATAAATGC ACAGTTTTCT AAGTTTGGGA AGCAGCACAT AGAGAACCTC 
TTCAGTGACC TACAGGATGG GAGACGCCTC CTAGACCTTT TGGAAGGCCT GACAGGGCAA 
AAACTGCCAA AAGAAAAAGG ATCCACAAGA GTTCATGCCC TGAACAATGT CAACAAGGCA 
CTGCGCGTCT TGCAGAAAAA TAATGTTGAT TTAGTGAACA TTGGAAGTAC TGACATAGTA 
GATGGAAATC ACAAACTGAC TCTTGGTTTG ATTTGGAATA TAATCCTCCA CTGGCAGGTC 
AAAAATGTAA TGAAAAATAT CATGGCTGGA TTGCAACAAA CCAACAGTGA AAAGATTCTC 
CTGAGCTGGG TCCGACAATC AACTCGTAAT TATCCACAGG TTAATGTCAT TAACTTCACC 
ACCAGCTGGT CTGATGGCCT GGCTTTGAAC GCTCTCATCC ACAGTCATAG GCCAGACCTG 
TTTGATTGGA ATAGTGTGGT TTGCCAGCAG TCAGCCACAC AACGCCTGGA ACATGCATTC 
AACATTGCCA AATATCAATT AGGCATAGAG AAACTGCTTG ATCCTGAAGA TGTTGCCACC 
ACTTATCCAG ATAAGAAGTC CATCTTAATG TATATCACAT CACTCTTCCA AGTTTTGCCT 
CAACAAGTGA GCATTGAAGC CATCCAGGAA GTGGAAATGT TGCCAAGGCC ATCAAAAGTT 
ACTAGAGAAG AACATTTTCA ATTACATCAT CAAATGCACT ATTCTCAACA GCATTTGGAA 
ACTCCTGAAG ACAAGTCATT TGGCCGGTCA TTGACAGAGA CCGAAGCAAA CCTGGACAGT 
TATCAAACAG CTTTGGAAGA AGTACTCTCG TGGCTTCTTT CAGCTGAGGA TGCACTGCAA 
GCCCAAGGAG AGATTTCTAA TGATGTCGAA GAAGTGAAAG AACAATTTCA TACTCATGAG 
GGATATATGA TGGACTTGAC ATCCCATCAG GGACGGGTCG GTAATGTTCT CCAACTGGGA 
AGTCAACTGA TTGGAACAGG GAAATTATCA GAAGATGAAG AAACCGAAGT GCAGGAACAA 
ATGAATCTCC TCAATTCAAG ATGGGAATGC CTCAGGGTAG CTAGCATGGA AAAACAAAGC 
AATTTACATA AAGTTCTAAT GGATCTCCAG AATCAGCAAC TGAAAGAGTT AAATGACTGG 
CTAACCAAAA CAGAAGAGAG AACAAGGAAA ATGGAGAAGG AGCCCCTTGG ACCTGATATT 
GAAGACCTAA AACGCCAAGT ACAACAACAT AAGGTGCTTC AAGAAGACTT AGAACAGGAA 
CAAGTCAGGG TCAATTCCCT CACTCATATG GTGGTGGTAG TCGATGAATC TAGTGGAGAC 
CATGCAACTG CTGCTTTGGA AGAACAACTT AAGGTACTGG GAGATCGATG GGCAAACATC 
TGTAGGTGGA CAGAAGATCG CTGGGTTCTT TTACAAGACA TCCTCCTAAA ATGGCAGCGT 
TTTACTGAAG AACAGTGCCT TTTCAGTGCA TGGCTTTCGG AGAAGGAAGA TGCAGTGAAC 
AAGATTCACA CAACTGGCTT TAAGGATCAA AGTGAAGTGT TATCAAATCT TCAGAAACTG 
GCTGTCTTAA AAACAGATCT GGAAAAGAAG AAGCAAACCA TGGACAAACT CTGCTCACTC 
AACCAAGACC TTCTTTCAGC GCTGAAAAAC ACAGTGGTAG CCCACAAGAT GGAAGCATGG 
CTGGACAACT TTGCCCAGCG CTGGGATAAT TTAGTCCAGA AACTTGAAAA AAGTTCAGCA 
CAGATTTCAC AGGCTGTCAC CACCACTCAG CCATCACTAA CACAGACAAC TGTAATGGAA 
ACAGTAACTA TGGTGACCAC GAGGGAACAC ATCTTGGTAA AGCATGCCCA AGAGGAACTG 
CCACCACCAC CCCCTCAGAA GAAGAGGCAG ATTATCGTGG ATTCTGAAAT TAGGAAAAGG 
TTGGATGTCG ATATAACTGA ACTTCACAGT TGGATTACTC GTTCAGAAGC TGTGTTGCAG 
AGTCCTGAAT TTGCAATCTA TCGGAAGGAA GGCAACTTCT CAGACCTTAA AGAAAAAGTC 
AATGCCATAG AGCGAGAAAA AGCCGAGAAG TTCAGAAAAC TGCAAGATGC CAGCAGATCA 
GCTCAGGCCC TGGTGGAACA GATGGTGAAT GAGGGTGTTA ATGCTGACAG CATCAAACAA 
GCCTCCGAAC AACTGAACAG CCGGTGGATA GAGTTCTGCC AATTGCTAAG CGAGAGACTT 
AACTGGCTGG AGTATCAGAA CAACATCATC ACTTTCTATA ATCAGCTACA ACAATTGGAG 
CAGATGACAA CTACTGCTGA AAACTGGTTG AAAACCCAGC CTACCACCAC ATCAGAGCCA 
ACAGCAATTA AAAGCCAGTT AAAAATTTGT AAGGATGAAA TCAACCGACT GTCAGCTCTT 
CAGCCTCAAA TCGAGCGATT AAAAATTCAA AGCATAGCCC TGAAAGAGAA AGGACAAGGG 
CCAATGTTCC TGGATGCAGA CTTTGTGGCC TTTACAAATC ATTTTAACCA AGTCTTTGCT 
GATGTGCAGG CAAGAGAAAA AGAGCTACAA ACAATTTTTG ACAGTTTGCC ACCCATGCGC 
TATCAGGAGA CTATGAGTAC CATCCTGACA TGGATCCAGC AGTCAGAAAC CAAACTCTCT 
ATACCTCAGG TTACTGTCAC TGAATATGAC ATCATGGAAC AGAGACTCGG AGAGCTACAG 
GCTTTACAAA GCTCTCTGCA AGAGCAACAA AATGGCCTAA ACTATCTCAG CACCACTGTG 
AAAGAGATGT CAAAGAAAGC ACCACTGTCT GATATTAGTC GGAAATATCA ATCAGAATTT 
GAAGAGATTG AGGGACGTTG GAAGAAGCTG TCTTCCCAGC TGGTTGAACA TTGTCAAAAG 
TTGGAGGAGC AAATGGCTAA ACTTCGAAAA ATTCAGAATC ACATAAAAAC TCTGAAGAAA 
TGGATCACTG AAGTCGATGT TTTCCTGAAG GAGGAATGGC CTGCCCTTGG GGATTCAGAA 
ATTCTGAAAA GACAGCTGAA ACAGTGCAGG CTTTTAGTCA ATGACATTCA GACCATCCAG 
CCTAGTCTCA ACAGTGTCAA TGAAGGGGCT CAGAAGATGA AGAATGAAGC AGAACCAGAG 
TTTGCTGGCA GACTTGAGAC AGAGCTCCGA GAACTTAACA CCCAGTGGGA TTACATGTGC 
CGCCAGGTCT ATGCCAGGAA GGAAGCCTTA AAAGGAGGTT TGGATAAAAC TGTAAGTCTT 
CAGAAAGATC TGTCAGAGAT GCATGAGTGG ATGACACAAG CTGAAGAAGA ATACCTAGAG 
AGAGATTTCG AATACAAGAC CCCTGATGAA TTACAGACAG CAGTTGAAGA GATGAAGAGA 
GCTAAAGAAG AGGCCCAGCA AAAAGAAGCA AAAGTGAAAC TCCTAACCGA GTCCGTCAAT 
AGTGTCATAG CTCAGGCTCC ACCTGCAGCA CAAGAGGCCT TAAAAAAGGA ACTTGACACT 
CTCACCACCA ACTACCAGTG GCTCTGCACC AGGCTCAATG GCAAATGCAA GACCTTGGAA 
GAAGTTTGGG CGTGCTGGCA TGAGTTATTG TCCTACTTGG AGAAGGCAAA CAAGTGGCTA 
AGTGAAGTAG AAGTCAAGCT TAAAACCACT GAAAATATTT CTGGGGGAGC TGAGGAAATC 
GCCGAGGTGC TTGATTCGCT TGAAAATTTG ATGCAACATT CAGAGGATAA CCCGAATCAG 
ATTCGCATAT TGGCACAGAC CTTGACAGAT GGTGGAGTCA TGGATGAACT GATCAATGAG 
GAGCTTGAGA CATTTAATTC TCGTTGGAGA GAACTCCATG AAGAGGCTGT GAGGAGGCAA 
AAGTTGCTTG AGCAGAGTAT CCAGTCGGCC CAGGAGATAG AAAAATCCTT GCACTTAATT 
CAGGAGTCCC TCTCTTCCAT TGACAAGCAG TTGGCAGCTT ATATTGCTGA CAAAGTGGAT 
GCAGCTCAGA TGCCTCAGGA AGCCCAGAAA ATCCAATCAG ATTTGACAAG TCATGAGATC 
AGTTTAGAAG AAATGAAGAA ACATAACCAG GGAAAGGAGA CTGCCCAAAG GGTACTATCC 
CAAATTGATG TGGCACAGAA AAAATTGCAG GATGTTTCCA TGAAGTTTCG ATTATTCCAG 
AAGCCAGCCA ATTTTGAGCA GCGCCTACAA GAAAGTAAAA TGATTTTAGA TGAAGTGAAG 
ATGCATTTAC CTGCGTTGGA AACAAAGAGT GTGGAACAGG AAGTAGTACA GTCACAGTTA 
AATCATTGTG TGAACTTGTA TAAAAGTCTG AGTGAAGTGA AGTCTGAAGT GGAAATGGTA 
ATAAAAACTG GACGTCAGAT TGTACAGAAG AAGCAGACGG AAAACCCGAA AGAGCTTGAT 
GAAAGAGTTA CAGCTTTGAA ATTGCATTAT AATGAGCTGG GAGCAAAGGT GACAGAAAGA 
AAGCAACAGT TGGAAAAATG CTTGAAATTG TCCCGTAAGA TGCGAAAGGA AATGAATGCC 
CTGACAGAAT GGCTGGCAGC GACAGATATG GAACTGACGA AGAGATCGGC AGTTGAAGGA 
ATGCCTAGTA ATTTGGATTC TGAAGTTGCC TGGGGAAAGG CTACTCAGAA AGAGATTGAG 
AAACAGAAGG TTCACCTAAA GAGTGTCACA GAGGTAGGAG AGGCCTTGAA AACGGTTTTG 
GGCAAGAAGG AAATGTTGGT GGAAGATAAA CTGAGTCTTC TGAATAGTAA CTGGATAGCC 
GTCACTTCCC GAGCAGAAGA GTGGTTAAAC CTTTTATTGG AATACCAGAA ACACATGGAA 
AACTTTGACC AGAATGTGGA TTACATCACA AACTGGATCA TTCAGGCTGA TGCACTTTTG 
GATGAATCTG AGAAAAAGAA ACCTCAGCAA AAAGAAGACA TACTTAAGCG TTTAAAGGCT 
GAAATGAATG ACATACGTCC AAAGGTGGAT TCTACACGTG ACCAAGCAGC AAACCTGATG 
GCAAACCGCG GCGACCACTG CAGGAAAGTA GTAGAGCCCA AAATCTCAGA GCTCAACCAT 
CGATTTGCAG CCATTTCTCA CAGAATTAAG ACTGGAAAGG CCTCCATTCC TTTGAAGGAA 
TTGGAGCAGT TTAACTCAGA TATACAAAAA TTGCTTGAAC CACTGGAGGC TGAAATTCAG 
CAGGGGGTGA ATCTGAAAGA GGAAGACTTC AATAAAGATA TGAGTGAAGA CAATGAGGGT 
ACTGTAAAAG AATTGTTGCA AAGAGGAGAC AACTTACAAC AAAGAATCAC AGATGAGAGA 
AAGCGAGAGG AAATAAAGAT AAAACAACAG CTGTTACAGA CAAAACATAA TGCTCTCAAG 
GATTTGAGGT CTCAAAGAAG AAAAAAGGCT CTAGAAATTT CTCACCAGTG GTATCAGTAC 
AAGAGGCAGG CTGATGATCT CCTGAAATGC TTGGATGACA TTGAAAAAAA ATTAGCCAGC 
CTACCTGAAC CCAGAGATGA AAGGAAAATA AAGGAAATTG ATCGTGAATT GCAGAAGAAG 
AAAGAGGAGC TGAATGCAGT GCGTAGGCAA GCTGAGGGCT TGTCTGAGGA TGGGGCCGCA 
ATGGCAGTGG AGCCAACTCA GATCCAGCTC AGCAAGCGCT GGCGGGAAAT TGAGAGCAAA 
TTTGCTCAGT TTCGAAGACT CAACTTTGCA CAAATTCACA CTGTCCATGA AGAGTCAGTG 
GTGGCGATGA CTGAAGACAT GCCTTTGGAA ATTTCTTATG TGCCTTCTAC TTACCTGACT 
GAGATCACTC ATGTCTCACA AGCCCTATCA GAAGTGGAAG AGCTTCTTAA TGCTCCCGAC 
CTTTGTGCTC AAGATTTTGA AGATCTCTTT AAACAAGAGG AATCCTTGAA GAACATAAAA 
GACAGCCTGC AACAAATCTC AGGTCGGATT GACATCATTC ACAATAAAAA GACAGCAGCA 
TTGCACAGTG CCACTCCTGC AGAAAGGGCA AAGCTCCAGG AAGCTCTCTC ACGGCTTGAT 
TTCCAATGGG AAAGAGTTAA CAATATGTAC AAGGACCGAC AAGGGAGATT TGACAGATCT 
GTGGAAAAAT GGCGGCGGTT TCATTATGAT ATGAAGATAC TTAATCAATG GCTAACAGAA 
GCTGAACAGT TTCTCAAAAA GACACAAATT CCTGAGAATT GGGAACATGC CAAATACAAA 
TGGTATCTTA AGGAACTCCA GGATGGCATT GGACAGCGGC AAAGTGTTGT CAGGGTATTG 
AATGCAACTG GGGAAGAAAT AATTCAACAG TCCTCAAAAA CAGATGCCAG TATTCTCCAA 
GAAAAACTGG GAAGCCTGAA TCTGCGGTGG CAGGAGGTCT GCAAACAGCT GGCAGAAAGA 
AAAAAGAGGC TAGAGGAACA GAAGAATATC TTGTCAGAAT TTCAAAGAGA TGTAAATGAA 
TTTGTTTTAT GGTTGGAAGA AGCGGATAAC GTTGCTAATA TTCCACTTGA ACCTGGAAAT 
GAGCAGCAGC TAAAAGAAAA ACTTGAACAA GTCAAGTTAC TGGCAGAAGA GTTGCCCCTG 
CGCCAGGGAA TTCTAAAACA ATTAAATGAA ACTGGAGGAA CAGTGCTTGT AAGTGCTCCC 
CTAAGCCCAG AAGAGCAAGA TAAACTTGAA AATAAGCTCA AGCAGACAAA TCTTCAGTGG 
ATAAAGGTTT CTAGAAATCT GCCTGAGAAG CAAGAAGAAA TTGAGGCACA CGTAAAAGAC 
CTTGGACAGC TGGAAGAGCA GTTAAATCAT CTGCTTCTAT GGCTGTCTCC TATTAGGAAT 
CAGTTGGAAA TTTACAATCA GCCAAATCAA ACAGGACCAT TTGACATCAA GGAAATTGAA 
GTAGCAGTTC AAGCTAAACA GCCGGATGTG GAAGGGATTT TGTCTAAAGG GCAGCATTTG 
TACAAGGAAA AACCAGCCAC TCAGCCAGCG AAGAGAAAGC TGGAAGATCT CAGCTCTGAT 
TGGAAGGTGG TAACTCAGTT GCTTCAAGAG CTGCGGGCAA AGCAGCCTGG CCCAGCTCCT 
GGACTGACCA CTGTCAGAGC CCCTCCCAGT CAGACTGTTA CTCTGGTGAC ACAACCCGCG 
GTTACCAAGG AAACTGCCAT CTCCAAACTA GAAATGCCAT CTTCATTGCT GTTGGAGGTA 
CCTGCACTGG CAGATTTCAA CCGAGCTTGG ACAGAACTTA CCGACTGGCT GTCTCTGCTT 
GATCGAGTTA TAAAATCACA GAGGGTGATG GTGGGTGATC TTGAAGACAT TAACGAGATG 
ATCATCAAGC AGAAGGCAAC GCTGCAGGAT TTGGAACAGA GGCGCCCCCA GTTGGAAGAA 
CTCATTACCG CTGCCCAGAA TTTGAAAAAC AAGACCAGCA ATCAAGAGGC TAGAACAATC 
ATTACTGATC GAATTGAAAG AATTCAGAGT CAGTGGGATG AAGTACAGGA ACATCTTCAG 
AACCGGAGGC AACAGTTGAA TGAAATGTTA AAGGATTCCA CACAATGGCT GGAAGCTAAA 
GAGGAGGCTG AGCAGGTGTT GGGGCAGGCC AGAGCCAAGC TTGAGTCATG GAAGGAGGCT 
CCCTACACAG TAGATGCAAT CCAAAAGAAA ATCACAGAAA CCAAGCAGTT GGCCAAAGAC 
CTCCGCCAGT GGCAGATAAA TGTAGATGTT GCAAATGATT TGGCACTGAA ACTTCTCCGA 
GATTATTCTG CAGATGATAC CAGAAAAGTA CACATGATAA CAGAGAACAT CAATGCCTCT 
TGGGCAAGCA TCCATAAAAG ATTGAGTGAG CGAGAGGCTG CTCTGGAAGA AACCCACAGA 
TTACTGCAAC AGTTCCCCTT GGACCTGGAG AAGTTCCTTG CCTGGCTTAC AGAAGCCGAA 
ACAACTGCCA ACGTCCTGCA GGATGCCACC CATAAGGAAA GGCTTCTAGA AGATTCCAAG 
GGAGTAAGAG AGCTGATGAA ACAATGGCAA GATCTCCAAG GAGAAATCGA AGCTCACACA 
GATATCTATC ACAACCTGGA CGAAAATGGC CAAAAAGTCC TGAGATCCCT GGAAGGTTCT 
GACGATGCAG CCTTGTTGCA AAGACGTTTG GATAACATGA ACTTCAAGTG GAGCGAACTT 
CGGAAAAAGT CTCTCAACAT TAGGTCTCAC TTGGAAGCCA GTTCTGACCA GTGGAAGCGT 
CTGCACCTTT CTCTTCAGGA ACTTCTGGTA TGGCTCCAGC TGAAAGATGA TGAGTTAAGC 
CGGCAGGCAC CCATTGGAGG AGACTTTCCA GCGGTGCAGA AGCAGAATGA TGTACACAGG 
GCCTTCAAGA GGGAATTGAA AACGAAAGAA CCTGTAATCA TGAGTACTCT TGAGACTGTA 
CGAATATTTC TGACAGAGCA GCCTTTAGAA GGACTAGAGA AACTCTACCA GGAGCCCAGA 
GAGCTGCCTC CTGAAGAGAG AGCCCAGAAT GTCACACGGC TCCTACGAAA GCAAGCTGAG 
GAGGTCAACA CTCAGTGGGA AAAACTGAAC GTGCACTCTG CAGACTGGCA GAGAAAAATA 
GACGAGGCCC TCGAAAGACT CCAGGAGCTT CAGGAAGCAA CAGATGAGCT GGATCTCAAA 
CTACGTCAGG CAGAGGTGAT CAAAGGATCC TGGCAGCCTG TGGGTGATCT CCTCATTGAC 
TCTCTCCAAG ATCACCTCGA AAAAGTCAAG GCGCTTCGAG GAGAAATTAC ACCTCTGAAA 
GAGAATGTCA GCTACGTCAA TGACCTTGCT CGCCAACTCA CTACGTTGGG CATTCAGCTG 
TCACCATATA ACCTCAACAC TCTGGAAGAC CTGAACACCA GATGGAAGCT TCTGCAGGTG 
GCCATTGAGG ACCGCATCAG GCAGCTGCAT GAAGCGCACA GGGACTTTGG ACCAGCCTCC 
CAGCACTTCC TTTCCACTTC TGTCCAGGGT CCCTGGGAGA GAGCCATCTC ACCAAACAAA 
GTGCCCTACT ATATCAACCA CGAGACCCAA ACAACTTGCT GGGACCATCC CAAAATGACA 
GAGCTCTACC AGTCTTTAGC TGACCTGAAT AATGTCAGGT TCTCAGCTTA CAGGACTGCC 
ATGAAACTCC GAAGACTGCA GAAGGCCCTT TGCTTGGATC TCTTGAGCCT ATCGGCTGCA 
TGCGATGCCT TGGACCAGCA CAACCTCAAG CAAAATGACC AGCCCATGGA TATCCTGCAG 
GTCATTAACT GTCTGACCAC TATTTATGAT CGCCTAGAGC AAGAGCACAA CAATCTGGTC 
AACGTCCCTC TCTGCGTGGA TATGTGTCTC AATTGGCTGC TGAATGTTTA TGACACGGGA 
CGAACGGGGA GGATCCGGGT CCTGTCTTTT AAAACTGGCA TCATTTCTCT GTGTAAAGCC 
CATTTGGAAG ACAAGTACAG ATACCTCTTC AAGCAAGTGG CAAGTTCGAC AGGATTTTGT 
GACCAGCGCA GGCTGGGCCT CCTCCTGCAT GACTCTATCC AGATCCCAAG ACAGTTGGGT 
GAAGTCGCAT CGTTCGGGGG CAGTAACATT GAGCCGAGTG TCAGGAGCTG CTTCCAGTTT 
GCTAATAATA AGCCTGAGAT CGAAGCGGCC CTCTTCCTAG ACTGGATGCG CCTGGAGCCC 
CAGTCCATGG TGTGGCTGCC TGTCCTGCAC CGAGTGGCTG CCGCGGAAAC TGCCAAGCAC 
CAGGCCAAGT GCAACATCTG CAAGGAGTGT CCCATCATCG GATTCAGGTA CAGGAGTCTA 
AAGCACTTTA ATTATGACAT CTGCCAAAGT TGCTTTTTTT CTGGTCGAGT TGCAAAAGGC 
CATAAAATGC ACTATCCCAT GGTGGAATAC TGCACTCCGA CTACATCGGG AGAAGATGTC 
CGTGACTTTG CCAAGGTACT AAAAAACAAA TTTCGAACCA AAAGGTATTT TGCGAAGCAT 
CCCCGAATGG GCTACCTGCC AGTGCAGACT GTCTTAGAGG GGGACAACAT GGAAACTCCT 
GTCACTCTGA TCAACTTCTG GCCGGTAGAT TCTGCGCCTG CCTCGTCCCC TCAGCTTTCA 
CACGATGATA CTCATTCACG CATTGAGCAT TATGCTAGCA GGCTAGCAGA AATGGAAAAC 
AGCAATGGAT CTTATCTAAA TGATAGCATC TCTCCTAATG AGAGCATAGA TGATGAACAT 
TTGTTAATCC AGCATTACTG CCAAAGTTTG AACCAGGACT CCCCCCTGAG CCAGCCTCGT 
AGTCCTGCCC AGATCTTGAT TTCCTTAGAG AGTGAGGAAA GAGGGGAGCT AGAGAGAATC 
CTAGCAGATC TTGAGGAAGA AAACAGAAAT CTGCAAGCAG AGTATGATCG TCTAAAGCAG 
CAGCATGAAC ACAAAGGCCT GTCCCCACTG CCATCCCCTC CTGAAATGAT GCCTACTTCT 
CCCCAAAGTC CCCGGGATGC TGAGCTCATC GCTGAGGCCA AGCTGCTGCG TCAACACAAA 
GGCCGCCTGG AAGCCAGGAT GCAAATCCTT GAAGACCATA ACAAACAACT GGAATCCCAG 
TTACACAGGC TCAGGCAGCT GCTGGAGCAA CCCCAGGCAG AGGCCAAGGT GAATGGTACA 
ACGGTGTCTT CTCCTTCTAC CTCTCTTCAG AGGTCAGATA GCAGTCAGCC TATGCTGCTC 
CGGGTGGTCG GCAGTCAGAC TTCAGAATCC ATGGGCGAGG AAGACCTGCT CAGCCCTCCC 
CAGGACACAA GCACAGGGTT AGAGGAAGTG ATGGAGCAGC TCAACCACTC CTTCCCTAGT 
TCCAGAGGAC ACAATGTAGG AAGCCTTTTC CACTTGGCAG ATGATTTGGG CAGAGCGATG 
GAGTCCTTAG TTTCAGTCAT GACAGATGAA GAAGGGGCAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMD ( XM_014111348.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013966823.1 Canis lupus familiaris dystrophin (DMD), transcript variant X10, mRNA.

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CloneID	OCe45419
Clone ID Related Accession (Same CDS sequence)	XM_014111348.1
Accession Version	XM_014111348.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	dystrophin isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTGGT GGGAAGAAGT AGAGGACTGT TATGAAAGAG AAGATGTTCA AAAGAAAACA 
TTCACAAAAT GGATAAATGC ACAGTTTTCT AAGTTTGGGA AGCAGCACAT AGAGAACCTC 
TTCAGTGACC TACAGGATGG GAGACGCCTC CTAGACCTTT TGGAAGGCCT GACAGGGCAA 
AAACTGCCAA AAGAAAAAGG ATCCACAAGA GTTCATGCCC TGAACAATGT CAACAAGGCA 
CTGCGCGTCT TGCAGAAAAA TAATGTTGAT TTAGTGAACA TTGGAAGTAC TGACATAGTA 
GATGGAAATC ACAAACTGAC TCTTGGTTTG ATTTGGAATA TAATCCTCCA CTGGCAGGTC 
AAAAATGTAA TGAAAAATAT CATGGCTGGA TTGCAACAAA CCAACAGTGA AAAGATTCTC 
CTGAGCTGGG TCCGACAATC AACTCGTAAT TATCCACAGG TTAATGTCAT TAACTTCACC 
ACCAGCTGGT CTGATGGCCT GGCTTTGAAC GCTCTCATCC ACAGTCATAG GCCAGACCTG 
TTTGATTGGA ATAGTGTGGT TTGCCAGCAG TCAGCCACAC AACGCCTGGA ACATGCATTC 
AACATTGCCA AATATCAATT AGGCATAGAG AAACTGCTTG ATCCTGAAGA TGTTGCCACC 
ACTTATCCAG ATAAGAAGTC CATCTTAATG TATATCACAT CACTCTTCCA AGTTTTGCCT 
CAACAAGTGA GCATTGAAGC CATCCAGGAA GTGGAAATGT TGCCAAGGCC ATCAAAAGTT 
ACTAGAGAAG AACATTTTCA ATTACATCAT CAAATGCACT ATTCTCAACA GATCACAGTC 
AGTCTAGCAC AGGGCTATGA ACGAGCCCCT TCCTCTCCTA AGCCTCGGTT CAAGAGCTAT 
GCCTACACAC AGGCTGCTTA TGTCACCACT TCTGACCCCA CACGGAGCCC ACTTCCTTCA 
CAGCATTTGG AAACTCCTGA AGACAAGTCA TTTGGCCGGT CATTGACAGA GACCGAAGCA 
AACCTGGACA GTTATCAAAC AGCTTTGGAA GAAGTACTCT CGTGGCTTCT TTCAGCTGAG 
GATGCACTGC AAGCCCAAGG AGAGATTTCT AATGATGTCG AAGAAGTGAA AGAACAATTT 
CATACTCATG AGGGATATAT GATGGACTTG ACATCCCATC AGGGACGGGT CGGTAATGTT 
CTCCAACTGG GAAGTCAACT GATTGGAACA GGGAAATTAT CAGAAGATGA AGAAACCGAA 
GTGCAGGAAC AAATGAATCT CCTCAATTCA AGATGGGAAT GCCTCAGGGT AGCTAGCATG 
GAAAAACAAA GCAATTTACA TAAAGTTCTA ATGGATCTCC AGAATCAGCA ACTGAAAGAG 
TTAAATGACT GGCTAACCAA AACAGAAGAG AGAACAAGGA AAATGGAGAA GGAGCCCCTT 
GGACCTGATA TTGAAGACCT AAAACGCCAA GTACAACAAC ATAAGGTGCT TCAAGAAGAC 
TTAGAACAGG AACAAGTCAG GGTCAATTCC CTCACTCATA TGGTGGTGGT AGTCGATGAA 
TCTAGTGGAG ACCATGCAAC TGCTGCTTTG GAAGAACAAC TTAAGGTACT GGGAGATCGA 
TGGGCAAACA TCTGTAGGTG GACAGAAGAT CGCTGGGTTC TTTTACAAGA CATCCTCCTA 
AAATGGCAGC GTTTTACTGA AGAACAGTGC CTTTTCAGTG CATGGCTTTC GGAGAAGGAA 
GATGCAGTGA ACAAGATTCA CACAACTGGC TTTAAGGATC AAAGTGAAGT GTTATCAAAT 
CTTCAGAAAC TGGCTGTCTT AAAAACAGAT CTGGAAAAGA AGAAGCAAAC CATGGACAAA 
CTCTGCTCAC TCAACCAAGA CCTTCTTTCA GCGCTGAAAA ACACAGTGGT AGCCCACAAG 
ATGGAAGCAT GGCTGGACAA CTTTGCCCAG CGCTGGGATA ATTTAGTCCA GAAACTTGAA 
AAAAGTTCAG CACAGATTTC ACAGGCTGTC ACCACCACTC AGCCATCACT AACACAGACA 
ACTGTAATGG AAACAGTAAC TATGGTGACC ACGAGGGAAC ACATCTTGGT AAAGCATGCC 
CAAGAGGAAC TGCCACCACC ACCCCCTCAG AAGAAGAGGC AGATTATCGT GGATTCTGAA 
ATTAGGAAAA GGTTGGATGT CGATATAACT GAACTTCACA GTTGGATTAC TCGTTCAGAA 
GCTGTGTTGC AGAGTCCTGA ATTTGCAATC TATCGGAAGG AAGGCAACTT CTCAGACCTT 
AAAGAAAAAG TCAATGCCAT AGAGCGAGAA AAAGCCGAGA AGTTCAGAAA ACTGCAAGAT 
GCCAGCAGAT CAGCTCAGGC CCTGGTGGAA CAGATGGTGA ATGAGGGTGT TAATGCTGAC 
AGCATCAAAC AAGCCTCCGA ACAACTGAAC AGCCGGTGGA TAGAGTTCTG CCAATTGCTA 
AGCGAGAGAC TTAACTGGCT GGAGTATCAG AACAACATCA TCACTTTCTA TAATCAGCTA 
CAACAATTGG AGCAGATGAC AACTACTGCT GAAAACTGGT TGAAAACCCA GCCTACCACC 
ACATCAGAGC CAACAGCAAT TAAAAGCCAG TTAAAAATTT GTAAGGATGA AATCAACCGA 
CTGTCAGCTC TTCAGCCTCA AATCGAGCGA TTAAAAATTC AAAGCATAGC CCTGAAAGAG 
AAAGGACAAG GGCCAATGTT CCTGGATGCA GACTTTGTGG CCTTTACAAA TCATTTTAAC 
CAAGTCTTTG CTGATGTGCA GGCAAGAGAA AAAGAGCTAC AAACAATTTT TGACAGTTTG 
CCACCCATGC GCTATCAGGA GACTATGAGT ACCATCCTGA CATGGATCCA GCAGTCAGAA 
ACCAAACTCT CTATACCTCA GGTTACTGTC ACTGAATATG ACATCATGGA ACAGAGACTC 
GGAGAGCTAC AGGCTTTACA AAGCTCTCTG CAAGAGCAAC AAAATGGCCT AAACTATCTC 
AGCACCACTG TGAAAGAGAT GTCAAAGAAA GCACCACTGT CTGATATTAG TCGGAAATAT 
CAATCAGAAT TTGAAGAGAT TGAGGGACGT TGGAAGAAGC TGTCTTCCCA GCTGGTTGAA 
CATTGTCAAA AGTTGGAGGA GCAAATGGCT AAACTTCGAA AAATTCAGAA TCACATAAAA 
ACTCTGAAGA AATGGATCAC TGAAGTCGAT GTTTTCCTGA AGGAGGAATG GCCTGCCCTT 
GGGGATTCAG AAATTCTGAA AAGACAGCTG AAACAGTGCA GGCTTTTAGT CAATGACATT 
CAGACCATCC AGCCTAGTCT CAACAGTGTC AATGAAGGGG CTCAGAAGAT GAAGAATGAA 
GCAGAACCAG AGTTTGCTGG CAGACTTGAG ACAGAGCTCC GAGAACTTAA CACCCAGTGG 
GATTACATGT GCCGCCAGGT CTATGCCAGG AAGGAAGCCT TAAAAGGAGG TTTGGATAAA 
ACTGTAAGTC TTCAGAAAGA TCTGTCAGAG ATGCATGAGT GGATGACACA AGCTGAAGAA 
GAATACCTAG AGAGAGATTT CGAATACAAG ACCCCTGATG AATTACAGAC AGCAGTTGAA 
GAGATGAAGA GAGCTAAAGA AGAGGCCCAG CAAAAAGAAG CAAAAGTGAA ACTCCTAACC 
GAGTCCGTCA ATAGTGTCAT AGCTCAGGCT CCACCTGCAG CACAAGAGGC CTTAAAAAAG 
GAACTTGACA CTCTCACCAC CAACTACCAG TGGCTCTGCA CCAGGCTCAA TGGCAAATGC 
AAGACCTTGG AAGAAGTTTG GGCGTGCTGG CATGAGTTAT TGTCCTACTT GGAGAAGGCA 
AACAAGTGGC TAAGTGAAGT AGAAGTCAAG CTTAAAACCA CTGAAAATAT TTCTGGGGGA 
GCTGAGGAAA TCGCCGAGGT GCTTGATTCG CTTGAAAATT TGATGCAACA TTCAGAGGAT 
AACCCGAATC AGATTCGCAT ATTGGCACAG ACCTTGACAG ATGGTGGAGT CATGGATGAA 
CTGATCAATG AGGAGCTTGA GACATTTAAT TCTCGTTGGA GAGAACTCCA TGAAGAGGCT 
GTGAGGAGGC AAAAGTTGCT TGAGCAGAGT ATCCAGTCGG CCCAGGAGAT AGAAAAATCC 
TTGCACTTAA TTCAGGAGTC CCTCTCTTCC ATTGACAAGC AGTTGGCAGC TTATATTGCT 
GACAAAGTGG ATGCAGCTCA GATGCCTCAG GAAGCCCAGA AAATCCAATC AGATTTGACA 
AGTCATGAGA TCAGTTTAGA AGAAATGAAG AAACATAACC AGGGAAAGGA GACTGCCCAA 
AGGGTACTAT CCCAAATTGA TGTGGCACAG AAAAAATTGC AGGATGTTTC CATGAAGTTT 
CGATTATTCC AGAAGCCAGC CAATTTTGAG CAGCGCCTAC AAGAAAGTAA AATGATTTTA 
GATGAAGTGA AGATGCATTT ACCTGCGTTG GAAACAAAGA GTGTGGAACA GGAAGTAGTA 
CAGTCACAGT TAAATCATTG TGTGAACTTG TATAAAAGTC TGAGTGAAGT GAAGTCTGAA 
GTGGAAATGG TAATAAAAAC TGGACGTCAG ATTGTACAGA AGAAGCAGAC GGAAAACCCG 
AAAGAGCTTG ATGAAAGAGT TACAGCTTTG AAATTGCATT ATAATGAGCT GGGAGCAAAG 
GTGACAGAAA GAAAGCAACA GTTGGAAAAA TGCTTGAAAT TGTCCCGTAA GATGCGAAAG 
GAAATGAATG CCCTGACAGA ATGGCTGGCA GCGACAGATA TGGAACTGAC GAAGAGATCG 
GCAGTTGAAG GAATGCCTAG TAATTTGGAT TCTGAAGTTG CCTGGGGAAA GGCTACTCAG 
AAAGAGATTG AGAAACAGAA GGTTCACCTA AAGAGTGTCA CAGAGGTAGG AGAGGCCTTG 
AAAACGGTTT TGGGCAAGAA GGAAATGTTG GTGGAAGATA AACTGAGTCT TCTGAATAGT 
AACTGGATAG CCGTCACTTC CCGAGCAGAA GAGTGGTTAA ACCTTTTATT GGAATACCAG 
AAACACATGG AAAACTTTGA CCAGAATGTG GATTACATCA CAAACTGGAT CATTCAGGCT 
GATGCACTTT TGGATGAATC TGAGAAAAAG AAACCTCAGC AAAAAGAAGA CATACTTAAG 
CGTTTAAAGG CTGAAATGAA TGACATACGT CCAAAGGTGG ATTCTACACG TGACCAAGCA 
GCAAACCTGA TGGCAAACCG CGGCGACCAC TGCAGGAAAG TAGTAGAGCC CAAAATCTCA 
GAGCTCAACC ATCGATTTGC AGCCATTTCT CACAGAATTA AGACTGGAAA GGCCTCCATT 
CCTTTGAAGG AATTGGAGCA GTTTAACTCA GATATACAAA AATTGCTTGA ACCACTGGAG 
GCTGAAATTC AGCAGGGGGT GAATCTGAAA GAGGAAGACT TCAATAAAGA TATGAGTGAA 
GACAATGAGG GTACTGTAAA AGAATTGTTG CAAAGAGGAG ACAACTTACA ACAAAGAATC 
ACAGATGAGA GAAAGCGAGA GGAAATAAAG ATAAAACAAC AGCTGTTACA GACAAAACAT 
AATGCTCTCA AGGATTTGAG GTCTCAAAGA AGAAAAAAGG CTCTAGAAAT TTCTCACCAG 
TGGTATCAGT ACAAGAGGCA GGCTGATGAT CTCCTGAAAT GCTTGGATGA CATTGAAAAA 
AAATTAGCCA GCCTACCTGA ACCCAGAGAT GAAAGGAAAA TAAAGGAAAT TGATCGTGAA 
TTGCAGAAGA AGAAAGAGGA GCTGAATGCA GTGCGTAGGC AAGCTGAGGG CTTGTCTGAG 
GATGGGGCCG CAATGGCAGT GGAGCCAACT CAGATCCAGC TCAGCAAGCG CTGGCGGGAA 
ATTGAGAGCA AATTTGCTCA GTTTCGAAGA CTCAACTTTG CACAAATTCA CACTGTCCAT 
GAAGAGTCAG TGGTGGCGAT GACTGAAGAC ATGCCTTTGG AAATTTCTTA TGTGCCTTCT 
ACTTACCTGA CTGAGATCAC TCATGTCTCA CAAGCCCTAT CAGAAGTGGA AGAGCTTCTT 
AATGCTCCCG ACCTTTGTGC TCAAGATTTT GAAGATCTCT TTAAACAAGA GGAATCCTTG 
AAGAACATAA AAGACAGCCT GCAACAAATC TCAGGTCGGA TTGACATCAT TCACAATAAA 
AAGACAGCAG CATTGCACAG TGCCACTCCT GCAGAAAGGG CAAAGCTCCA GGAAGCTCTC 
TCACGGCTTG ATTTCCAATG GGAAAGAGTT AACAATATGT ACAAGGACCG ACAAGGGAGA 
TTTGACAGAT CTGTGGAAAA ATGGCGGCGG TTTCATTATG ATATGAAGAT ACTTAATCAA 
TGGCTAACAG AAGCTGAACA GTTTCTCAAA AAGACACAAA TTCCTGAGAA TTGGGAACAT 
GCCAAATACA AATGGTATCT TAAGGAACTC CAGGATGGCA TTGGACAGCG GCAAAGTGTT 
GTCAGGGTAT TGAATGCAAC TGGGGAAGAA ATAATTCAAC AGTCCTCAAA AACAGATGCC 
AGTATTCTCC AAGAAAAACT GGGAAGCCTG AATCTGCGGT GGCAGGAGGT CTGCAAACAG 
CTGGCAGAAA GAAAAAAGAG GCTAGAGGAA CAGAAGAATA TCTTGTCAGA ATTTCAAAGA 
GATGTAAATG AATTTGTTTT ATGGTTGGAA GAAGCGGATA ACGTTGCTAA TATTCCACTT 
GAACCTGGAA ATGAGCAGCA GCTAAAAGAA AAACTTGAAC AAGTCAAGTT ACTGGCAGAA 
GAGTTGCCCC TGCGCCAGGG AATTCTAAAA CAATTAAATG AAACTGGAGG AACAGTGCTT 
GTAAGTGCTC CCCTAAGCCC AGAAGAGCAA GATAAACTTG AAAATAAGCT CAAGCAGACA 
AATCTTCAGT GGATAAAGGT TTCTAGAAAT CTGCCTGAGA AGCAAGAAGA AATTGAGGCA 
CACGTAAAAG ACCTTGGACA GCTGGAAGAG CAGTTAAATC ATCTGCTTCT ATGGCTGTCT 
CCTATTAGGA ATCAGTTGGA AATTTACAAT CAGCCAAATC AAACAGGACC ATTTGACATC 
AAGGAAATTG AAGTAGCAGT TCAAGCTAAA CAGCCGGATG TGGAAGGGAT TTTGTCTAAA 
GGGCAGCATT TGTACAAGGA AAAACCAGCC ACTCAGCCAG CGAAGAGAAA GCTGGAAGAT 
CTCAGCTCTG ATTGGAAGGT GGTAACTCAG TTGCTTCAAG AGCTGCGGGC AAAGCAGCCT 
GGCCCAGCTC CTGGACTGAC CACTGTCAGA GCCCCTCCCA GTCAGACTGT TACTCTGGTG 
ACACAACCCG CGGTTACCAA GGAAACTGCC ATCTCCAAAC TAGAAATGCC ATCTTCATTG 
CTGTTGGAGG TACCTGCACT GGCAGATTTC AACCGAGCTT GGACAGAACT TACCGACTGG 
CTGTCTCTGC TTGATCGAGT TATAAAATCA CAGAGGGTGA TGGTGGGTGA TCTTGAAGAC 
ATTAACGAGA TGATCATCAA GCAGAAGGCA ACGCTGCAGG ATTTGGAACA GAGGCGCCCC 
CAGTTGGAAG AACTCATTAC CGCTGCCCAG AATTTGAAAA ACAAGACCAG CAATCAAGAG 
GCTAGAACAA TCATTACTGA TCGAATTGAA AGAATTCAGA GTCAGTGGGA TGAAGTACAG 
GAACATCTTC AGAACCGGAG GCAACAGTTG AATGAAATGT TAAAGGATTC CACACAATGG 
CTGGAAGCTA AAGAGGAGGC TGAGCAGGTG TTGGGGCAGG CCAGAGCCAA GCTTGAGTCA 
TGGAAGGAGG CTCCCTACAC AGTAGATGCA ATCCAAAAGA AAATCACAGA AACCAAGCAG 
TTGGCCAAAG ACCTCCGCCA GTGGCAGATA AATGTAGATG TTGCAAATGA TTTGGCACTG 
AAACTTCTCC GAGATTATTC TGCAGATGAT ACCAGAAAAG TACACATGAT AACAGAGAAC 
ATCAATGCCT CTTGGGCAAG CATCCATAAA AGATTGAGTG AGCGAGAGGC TGCTCTGGAA 
GAAACCCACA GATTACTGCA ACAGTTCCCC TTGGACCTGG AGAAGTTCCT TGCCTGGCTT 
ACAGAAGCCG AAACAACTGC CAACGTCCTG CAGGATGCCA CCCATAAGGA AAGGCTTCTA 
GAAGATTCCA AGGGAGTAAG AGAGCTGATG AAACAATGGC AAGATCTCCA AGGAGAAATC 
GAAGCTCACA CAGATATCTA TCACAACCTG GACGAAAATG GCCAAAAAGT CCTGAGATCC 
CTGGAAGGTT CTGACGATGC AGCCTTGTTG CAAAGACGTT TGGATAACAT GAACTTCAAG 
TGGAGCGAAC TTCGGAAAAA GTCTCTCAAC ATTAGGTCTC ACTTGGAAGC CAGTTCTGAC 
CAGTGGAAGC GTCTGCACCT TTCTCTTCAG GAACTTCTGG TATGGCTCCA GCTGAAAGAT 
GATGAGTTAA GCCGGCAGGC ACCCATTGGA GGAGACTTTC CAGCGGTGCA GAAGCAGAAT 
GATGTACACA GGGCCTTCAA GAGGGAATTG AAAACGAAAG AACCTGTAAT CATGAGTACT 
CTTGAGACTG TACGAATATT TCTGACAGAG CAGCCTTTAG AAGGACTAGA GAAACTCTAC 
CAGGAGCCCA GAGAGCTGCC TCCTGAAGAG AGAGCCCAGA ATGTCACACG GCTCCTACGA 
AAGCAAGCTG AGGAGGTCAA CACTCAGTGG GAAAAACTGA ACGTGCACTC TGCAGACTGG 
CAGAGAAAAA TAGACGAGGC CCTCGAAAGA CTCCAGGAGC TTCAGGAAGC AACAGATGAG 
CTGGATCTCA AACTACGTCA GGCAGAGGTG ATCAAAGGAT CCTGGCAGCC TGTGGGTGAT 
CTCCTCATTG ACTCTCTCCA AGATCACCTC GAAAAAGTCA AGGCGCTTCG AGGAGAAATT 
ACACCTCTGA AAGAGAATGT CAGCTACGTC AATGACCTTG CTCGCCAACT CACTACGTTG 
GGCATTCAGC TGTCACCATA TAACCTCAAC ACTCTGGAAG ACCTGAACAC CAGATGGAAG 
CTTCTGCAGG TGGCCATTGA GGACCGCATC AGGCAGCTGC ATGAAGCGCA CAGGGACTTT 
GGACCAGCCT CCCAGCACTT CCTTTCCACT TCTGTCCAGG GTCCCTGGGA GAGAGCCATC 
TCACCAAACA AAGTGCCCTA CTATATCAAC CACGAGACCC AAACAACTTG CTGGGACCAT 
CCCAAAATGA CAGAGCTCTA CCAGTCTTTA GCTGACCTGA ATAATGTCAG GTTCTCAGCT 
TACAGGACTG CCATGAAACT CCGAAGACTG CAGAAGGCCC TTTGCTTGGA TCTCTTGAGC 
CTATCGGCTG CATGCGATGC CTTGGACCAG CACAACCTCA AGCAAAATGA CCAGCCCATG 
GATATCCTGC AGGTCATTAA CTGTCTGACC ACTATTTATG ATCGCCTAGA GCAAGAGCAC 
AACAATCTGG TCAACGTCCC TCTCTGCGTG GATATGTGTC TCAATTGGCT GCTGAATGTT 
TATGACACGG GACGAACGGG GAGGATCCGG GTCCTGTCTT TTAAAACTGG CATCATTTCT 
CTGTGTAAAG CCCATTTGGA AGACAAGTAC AGATACCTCT TCAAGCAAGT GGCAAGTTCG 
ACAGGATTTT GTGACCAGCG CAGGCTGGGC CTCCTCCTGC ATGACTCTAT CCAGATCCCA 
AGACAGTTGG GTGAAGTCGC ATCGTTCGGG GGCAGTAACA TTGAGCCGAG TGTCAGGAGC 
TGCTTCCAGT TTGCTAATAA TAAGCCTGAG ATCGAAGCGG CCCTCTTCCT AGACTGGATG 
CGCCTGGAGC CCCAGTCCAT GGTGTGGCTG CCTGTCCTGC ACCGAGTGGC TGCCGCGGAA 
ACTGCCAAGC ACCAGGCCAA GTGCAACATC TGCAAGGAGT GTCCCATCAT CGGATTCAGG 
TACAGGAGTC TAAAGCACTT TAATTATGAC ATCTGCCAAA GTTGCTTTTT TTCTGGTCGA 
GTTGCAAAAG GCCATAAAAT GCACTATCCC ATGGTGGAAT ACTGCACTCC GACTACATCG 
GGAGAAGATG TCCGTGACTT TGCCAAGGTA CTAAAAAACA AATTTCGAAC CAAAAGGTAT 
TTTGCGAAGC ATCCCCGAAT GGGCTACCTG CCAGTGCAGA CTGTCTTAGA GGGGGACAAC 
ATGGAAACAA ATCTGCAAGC AGAGTATGAT CGTCTAAAGC AGCAGCATGA ACACAAAGGC 
CTGTCCCCAC TGCCATCCCC TCCTGAAATG ATGCCTACTT CTCCCCAAAG TCCCCGGGAT 
GCTGAGCTCA TCGCTGAGGC CAAGCTGCTG CGTCAACACA AAGGCCGCCT GGAAGCCAGG 
ATGCAAATCC TTGAAGACCA TAACAAACAA CTGGAATCCC AGTTACACAG GCTCAGGCAG 
CTGCTGGAGC AACCCCAGGC AGAGGCCAAG GTGAATGGTA CAACGGTGTC TTCTCCTTCT 
ACCTCTCTTC AGAGGTCAGA TAGCAGTCAG CCTATGCTGC TCCGGGTGGT CGGCAGTCAG 
ACTTCAGAAT CCATGGGCGA GGAAGACCTG CTCAGCCCTC CCCAGGACAC AAGCACAGGG 
TTAGAGGAAG TGATGGAGCA GCTCAACCAC TCCTTCCCTA GTTCCAGAGG AAGAAATACC 
CCTGGGAAGC CAATGAGAGA GGACACAATG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013966823.1
CDS	248..10960
Translation	MLWWEEVEDCYEREDVQKKTFTKWINAQFSKFGKQHIENLFSDLQDGRRLLDLLEGLTGQKLPKEKGSTRVHALNNVNKALRVLQKNNVDLVNIGSTDIVDGNHKLTLGLIWNIILHWQVKNVMKNIMAGLQQTNSEKILLSWVRQSTRNYPQVNVINFTTSWSDGLALNALIHSHRPDLFDWNSVVCQQSATQRLEHAFNIAKYQLGIEKLLDPEDVATTYPDKKSILMYITSLFQVLPQQVSIEAIQEVEMLPRPSKVTREEHFQLHHQMHYSQQITVSLAQGYERAPSSPKPRFKSYAYTQAAYVTTSDPTRSPLPSQHLETPEDKSFGRSLTETEANLDSYQTALEEVLSWLLSAEDALQAQGEISNDVEEVKEQFHTHEGYMMDLTSHQGRVGNVLQLGSQLIGTGKLSEDEETEVQEQMNLLNSRWECLRVASMEKQSNLHKVLMDLQNQQLKELNDWLTKTEERTRKMEKEPLGPDIEDLKRQVQQHKVLQEDLEQEQVRVNSLTHMVVVVDESSGDHATAALEEQLKVLGDRWANICRWTEDRWVLLQDILLKWQRFTEEQCLFSAWLSEKEDAVNKIHTTGFKDQSEVLSNLQKLAVLKTDLEKKKQTMDKLCSLNQDLLSALKNTVVAHKMEAWLDNFAQRWDNLVQKLEKSSAQISQAVTTTQPSLTQTTVMETVTMVTTREHILVKHAQEELPPPPPQKKRQIIVDSEIRKRLDVDITELHSWITRSEAVLQSPEFAIYRKEGNFSDLKEKVNAIEREKAEKFRKLQDASRSAQALVEQMVNEGVNADSIKQASEQLNSRWIEFCQLLSERLNWLEYQNNIITFYNQLQQLEQMTTTAENWLKTQPTTTSEPTAIKSQLKICKDEINRLSALQPQIERLKIQSIALKEKGQGPMFLDADFVAFTNHFNQVFADVQAREKELQTIFDSLPPMRYQETMSTILTWIQQSETKLSIPQVTVTEYDIMEQRLGELQALQSSLQEQQNGLNYLSTTVKEMSKKAPLSDISRKYQSEFEEIEGRWKKLSSQLVEHCQKLEEQMAKLRKIQNHIKTLKKWITEVDVFLKEEWPALGDSEILKRQLKQCRLLVNDIQTIQPSLNSVNEGAQKMKNEAEPEFAGRLETELRELNTQWDYMCRQVYARKEALKGGLDKTVSLQKDLSEMHEWMTQAEEEYLERDFEYKTPDELQTAVEEMKRAKEEAQQKEAKVKLLTESVNSVIAQAPPAAQEALKKELDTLTTNYQWLCTRLNGKCKTLEEVWACWHELLSYLEKANKWLSEVEVKLKTTENISGGAEEIAEVLDSLENLMQHSEDNPNQIRILAQTLTDGGVMDELINEELETFNSRWRELHEEAVRRQKLLEQSIQSAQEIEKSLHLIQESLSSIDKQLAAYIADKVDAAQMPQEAQKIQSDLTSHEISLEEMKKHNQGKETAQRVLSQIDVAQKKLQDVSMKFRLFQKPANFEQRLQESKMILDEVKMHLPALETKSVEQEVVQSQLNHCVNLYKSLSEVKSEVEMVIKTGRQIVQKKQTENPKELDERVTALKLHYNELGAKVTERKQQLEKCLKLSRKMRKEMNALTEWLAATDMELTKRSAVEGMPSNLDSEVAWGKATQKEIEKQKVHLKSVTEVGEALKTVLGKKEMLVEDKLSLLNSNWIAVTSRAEEWLNLLLEYQKHMENFDQNVDYITNWIIQADALLDESEKKKPQQKEDILKRLKAEMNDIRPKVDSTRDQAANLMANRGDHCRKVVEPKISELNHRFAAISHRIKTGKASIPLKELEQFNSDIQKLLEPLEAEIQQGVNLKEEDFNKDMSEDNEGTVKELLQRGDNLQQRITDERKREEIKIKQQLLQTKHNALKDLRSQRRKKALEISHQWYQYKRQADDLLKCLDDIEKKLASLPEPRDERKIKEIDRELQKKKEELNAVRRQAEGLSEDGAAMAVEPTQIQLSKRWREIESKFAQFRRLNFAQIHTVHEESVVAMTEDMPLEISYVPSTYLTEITHVSQALSEVEELLNAPDLCAQDFEDLFKQEESLKNIKDSLQQISGRIDIIHNKKTAALHSATPAERAKLQEALSRLDFQWERVNNMYKDRQGRFDRSVEKWRRFHYDMKILNQWLTEAEQFLKKTQIPENWEHAKYKWYLKELQDGIGQRQSVVRVLNATGEEIIQQSSKTDASILQEKLGSLNLRWQEVCKQLAERKKRLEEQKNILSEFQRDVNEFVLWLEEADNVANIPLEPGNEQQLKEKLEQVKLLAEELPLRQGILKQLNETGGTVLVSAPLSPEEQDKLENKLKQTNLQWIKVSRNLPEKQEEIEAHVKDLGQLEEQLNHLLLWLSPIRNQLEIYNQPNQTGPFDIKEIEVAVQAKQPDVEGILSKGQHLYKEKPATQPAKRKLEDLSSDWKVVTQLLQELRAKQPGPAPGLTTVRAPPSQTVTLVTQPAVTKETAISKLEMPSSLLLEVPALADFNRAWTELTDWLSLLDRVIKSQRVMVGDLEDINEMIIKQKATLQDLEQRRPQLEELITAAQNLKNKTSNQEARTIITDRIERIQSQWDEVQEHLQNRRQQLNEMLKDSTQWLEAKEEAEQVLGQARAKLESWKEAPYTVDAIQKKITETKQLAKDLRQWQINVDVANDLALKLLRDYSADDTRKVHMITENINASWASIHKRLSEREAALEETHRLLQQFPLDLEKFLAWLTEAETTANVLQDATHKERLLEDSKGVRELMKQWQDLQGEIEAHTDIYHNLDENGQKVLRSLEGSDDAALLQRRLDNMNFKWSELRKKSLNIRSHLEASSDQWKRLHLSLQELLVWLQLKDDELSRQAPIGGDFPAVQKQNDVHRAFKRELKTKEPVIMSTLETVRIFLTEQPLEGLEKLYQEPRELPPEERAQNVTRLLRKQAEEVNTQWEKLNVHSADWQRKIDEALERLQELQEATDELDLKLRQAEVIKGSWQPVGDLLIDSLQDHLEKVKALRGEITPLKENVSYVNDLARQLTTLGIQLSPYNLNTLEDLNTRWKLLQVAIEDRIRQLHEAHRDFGPASQHFLSTSVQGPWERAISPNKVPYYINHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQVINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETNLQAEYDRLKQQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDTSTGLEEVMEQLNHSFPSSRGRNTPGKPMREDTM

Target ORF information:

RefSeq Version	XM_014111348.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris dystrophin (DMD), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111348.1

ORF Insert Sequence:

ATGCTTTGGT GGGAAGAAGT AGAGGACTGT TATGAAAGAG AAGATGTTCA AAAGAAAACA 
TTCACAAAAT GGATAAATGC ACAGTTTTCT AAGTTTGGGA AGCAGCACAT AGAGAACCTC 
TTCAGTGACC TACAGGATGG GAGACGCCTC CTAGACCTTT TGGAAGGCCT GACAGGGCAA 
AAACTGCCAA AAGAAAAAGG ATCCACAAGA GTTCATGCCC TGAACAATGT CAACAAGGCA 
CTGCGCGTCT TGCAGAAAAA TAATGTTGAT TTAGTGAACA TTGGAAGTAC TGACATAGTA 
GATGGAAATC ACAAACTGAC TCTTGGTTTG ATTTGGAATA TAATCCTCCA CTGGCAGGTC 
AAAAATGTAA TGAAAAATAT CATGGCTGGA TTGCAACAAA CCAACAGTGA AAAGATTCTC 
CTGAGCTGGG TCCGACAATC AACTCGTAAT TATCCACAGG TTAATGTCAT TAACTTCACC 
ACCAGCTGGT CTGATGGCCT GGCTTTGAAC GCTCTCATCC ACAGTCATAG GCCAGACCTG 
TTTGATTGGA ATAGTGTGGT TTGCCAGCAG TCAGCCACAC AACGCCTGGA ACATGCATTC 
AACATTGCCA AATATCAATT AGGCATAGAG AAACTGCTTG ATCCTGAAGA TGTTGCCACC 
ACTTATCCAG ATAAGAAGTC CATCTTAATG TATATCACAT CACTCTTCCA AGTTTTGCCT 
CAACAAGTGA GCATTGAAGC CATCCAGGAA GTGGAAATGT TGCCAAGGCC ATCAAAAGTT 
ACTAGAGAAG AACATTTTCA ATTACATCAT CAAATGCACT ATTCTCAACA GATCACAGTC 
AGTCTAGCAC AGGGCTATGA ACGAGCCCCT TCCTCTCCTA AGCCTCGGTT CAAGAGCTAT 
GCCTACACAC AGGCTGCTTA TGTCACCACT TCTGACCCCA CACGGAGCCC ACTTCCTTCA 
CAGCATTTGG AAACTCCTGA AGACAAGTCA TTTGGCCGGT CATTGACAGA GACCGAAGCA 
AACCTGGACA GTTATCAAAC AGCTTTGGAA GAAGTACTCT CGTGGCTTCT TTCAGCTGAG 
GATGCACTGC AAGCCCAAGG AGAGATTTCT AATGATGTCG AAGAAGTGAA AGAACAATTT 
CATACTCATG AGGGATATAT GATGGACTTG ACATCCCATC AGGGACGGGT CGGTAATGTT 
CTCCAACTGG GAAGTCAACT GATTGGAACA GGGAAATTAT CAGAAGATGA AGAAACCGAA 
GTGCAGGAAC AAATGAATCT CCTCAATTCA AGATGGGAAT GCCTCAGGGT AGCTAGCATG 
GAAAAACAAA GCAATTTACA TAAAGTTCTA ATGGATCTCC AGAATCAGCA ACTGAAAGAG 
TTAAATGACT GGCTAACCAA AACAGAAGAG AGAACAAGGA AAATGGAGAA GGAGCCCCTT 
GGACCTGATA TTGAAGACCT AAAACGCCAA GTACAACAAC ATAAGGTGCT TCAAGAAGAC 
TTAGAACAGG AACAAGTCAG GGTCAATTCC CTCACTCATA TGGTGGTGGT AGTCGATGAA 
TCTAGTGGAG ACCATGCAAC TGCTGCTTTG GAAGAACAAC TTAAGGTACT GGGAGATCGA 
TGGGCAAACA TCTGTAGGTG GACAGAAGAT CGCTGGGTTC TTTTACAAGA CATCCTCCTA 
AAATGGCAGC GTTTTACTGA AGAACAGTGC CTTTTCAGTG CATGGCTTTC GGAGAAGGAA 
GATGCAGTGA ACAAGATTCA CACAACTGGC TTTAAGGATC AAAGTGAAGT GTTATCAAAT 
CTTCAGAAAC TGGCTGTCTT AAAAACAGAT CTGGAAAAGA AGAAGCAAAC CATGGACAAA 
CTCTGCTCAC TCAACCAAGA CCTTCTTTCA GCGCTGAAAA ACACAGTGGT AGCCCACAAG 
ATGGAAGCAT GGCTGGACAA CTTTGCCCAG CGCTGGGATA ATTTAGTCCA GAAACTTGAA 
AAAAGTTCAG CACAGATTTC ACAGGCTGTC ACCACCACTC AGCCATCACT AACACAGACA 
ACTGTAATGG AAACAGTAAC TATGGTGACC ACGAGGGAAC ACATCTTGGT AAAGCATGCC 
CAAGAGGAAC TGCCACCACC ACCCCCTCAG AAGAAGAGGC AGATTATCGT GGATTCTGAA 
ATTAGGAAAA GGTTGGATGT CGATATAACT GAACTTCACA GTTGGATTAC TCGTTCAGAA 
GCTGTGTTGC AGAGTCCTGA ATTTGCAATC TATCGGAAGG AAGGCAACTT CTCAGACCTT 
AAAGAAAAAG TCAATGCCAT AGAGCGAGAA AAAGCCGAGA AGTTCAGAAA ACTGCAAGAT 
GCCAGCAGAT CAGCTCAGGC CCTGGTGGAA CAGATGGTGA ATGAGGGTGT TAATGCTGAC 
AGCATCAAAC AAGCCTCCGA ACAACTGAAC AGCCGGTGGA TAGAGTTCTG CCAATTGCTA 
AGCGAGAGAC TTAACTGGCT GGAGTATCAG AACAACATCA TCACTTTCTA TAATCAGCTA 
CAACAATTGG AGCAGATGAC AACTACTGCT GAAAACTGGT TGAAAACCCA GCCTACCACC 
ACATCAGAGC CAACAGCAAT TAAAAGCCAG TTAAAAATTT GTAAGGATGA AATCAACCGA 
CTGTCAGCTC TTCAGCCTCA AATCGAGCGA TTAAAAATTC AAAGCATAGC CCTGAAAGAG 
AAAGGACAAG GGCCAATGTT CCTGGATGCA GACTTTGTGG CCTTTACAAA TCATTTTAAC 
CAAGTCTTTG CTGATGTGCA GGCAAGAGAA AAAGAGCTAC AAACAATTTT TGACAGTTTG 
CCACCCATGC GCTATCAGGA GACTATGAGT ACCATCCTGA CATGGATCCA GCAGTCAGAA 
ACCAAACTCT CTATACCTCA GGTTACTGTC ACTGAATATG ACATCATGGA ACAGAGACTC 
GGAGAGCTAC AGGCTTTACA AAGCTCTCTG CAAGAGCAAC AAAATGGCCT AAACTATCTC 
AGCACCACTG TGAAAGAGAT GTCAAAGAAA GCACCACTGT CTGATATTAG TCGGAAATAT 
CAATCAGAAT TTGAAGAGAT TGAGGGACGT TGGAAGAAGC TGTCTTCCCA GCTGGTTGAA 
CATTGTCAAA AGTTGGAGGA GCAAATGGCT AAACTTCGAA AAATTCAGAA TCACATAAAA 
ACTCTGAAGA AATGGATCAC TGAAGTCGAT GTTTTCCTGA AGGAGGAATG GCCTGCCCTT 
GGGGATTCAG AAATTCTGAA AAGACAGCTG AAACAGTGCA GGCTTTTAGT CAATGACATT 
CAGACCATCC AGCCTAGTCT CAACAGTGTC AATGAAGGGG CTCAGAAGAT GAAGAATGAA 
GCAGAACCAG AGTTTGCTGG CAGACTTGAG ACAGAGCTCC GAGAACTTAA CACCCAGTGG 
GATTACATGT GCCGCCAGGT CTATGCCAGG AAGGAAGCCT TAAAAGGAGG TTTGGATAAA 
ACTGTAAGTC TTCAGAAAGA TCTGTCAGAG ATGCATGAGT GGATGACACA AGCTGAAGAA 
GAATACCTAG AGAGAGATTT CGAATACAAG ACCCCTGATG AATTACAGAC AGCAGTTGAA 
GAGATGAAGA GAGCTAAAGA AGAGGCCCAG CAAAAAGAAG CAAAAGTGAA ACTCCTAACC 
GAGTCCGTCA ATAGTGTCAT AGCTCAGGCT CCACCTGCAG CACAAGAGGC CTTAAAAAAG 
GAACTTGACA CTCTCACCAC CAACTACCAG TGGCTCTGCA CCAGGCTCAA TGGCAAATGC 
AAGACCTTGG AAGAAGTTTG GGCGTGCTGG CATGAGTTAT TGTCCTACTT GGAGAAGGCA 
AACAAGTGGC TAAGTGAAGT AGAAGTCAAG CTTAAAACCA CTGAAAATAT TTCTGGGGGA 
GCTGAGGAAA TCGCCGAGGT GCTTGATTCG CTTGAAAATT TGATGCAACA TTCAGAGGAT 
AACCCGAATC AGATTCGCAT ATTGGCACAG ACCTTGACAG ATGGTGGAGT CATGGATGAA 
CTGATCAATG AGGAGCTTGA GACATTTAAT TCTCGTTGGA GAGAACTCCA TGAAGAGGCT 
GTGAGGAGGC AAAAGTTGCT TGAGCAGAGT ATCCAGTCGG CCCAGGAGAT AGAAAAATCC 
TTGCACTTAA TTCAGGAGTC CCTCTCTTCC ATTGACAAGC AGTTGGCAGC TTATATTGCT 
GACAAAGTGG ATGCAGCTCA GATGCCTCAG GAAGCCCAGA AAATCCAATC AGATTTGACA 
AGTCATGAGA TCAGTTTAGA AGAAATGAAG AAACATAACC AGGGAAAGGA GACTGCCCAA 
AGGGTACTAT CCCAAATTGA TGTGGCACAG AAAAAATTGC AGGATGTTTC CATGAAGTTT 
CGATTATTCC AGAAGCCAGC CAATTTTGAG CAGCGCCTAC AAGAAAGTAA AATGATTTTA 
GATGAAGTGA AGATGCATTT ACCTGCGTTG GAAACAAAGA GTGTGGAACA GGAAGTAGTA 
CAGTCACAGT TAAATCATTG TGTGAACTTG TATAAAAGTC TGAGTGAAGT GAAGTCTGAA 
GTGGAAATGG TAATAAAAAC TGGACGTCAG ATTGTACAGA AGAAGCAGAC GGAAAACCCG 
AAAGAGCTTG ATGAAAGAGT TACAGCTTTG AAATTGCATT ATAATGAGCT GGGAGCAAAG 
GTGACAGAAA GAAAGCAACA GTTGGAAAAA TGCTTGAAAT TGTCCCGTAA GATGCGAAAG 
GAAATGAATG CCCTGACAGA ATGGCTGGCA GCGACAGATA TGGAACTGAC GAAGAGATCG 
GCAGTTGAAG GAATGCCTAG TAATTTGGAT TCTGAAGTTG CCTGGGGAAA GGCTACTCAG 
AAAGAGATTG AGAAACAGAA GGTTCACCTA AAGAGTGTCA CAGAGGTAGG AGAGGCCTTG 
AAAACGGTTT TGGGCAAGAA GGAAATGTTG GTGGAAGATA AACTGAGTCT TCTGAATAGT 
AACTGGATAG CCGTCACTTC CCGAGCAGAA GAGTGGTTAA ACCTTTTATT GGAATACCAG 
AAACACATGG AAAACTTTGA CCAGAATGTG GATTACATCA CAAACTGGAT CATTCAGGCT 
GATGCACTTT TGGATGAATC TGAGAAAAAG AAACCTCAGC AAAAAGAAGA CATACTTAAG 
CGTTTAAAGG CTGAAATGAA TGACATACGT CCAAAGGTGG ATTCTACACG TGACCAAGCA 
GCAAACCTGA TGGCAAACCG CGGCGACCAC TGCAGGAAAG TAGTAGAGCC CAAAATCTCA 
GAGCTCAACC ATCGATTTGC AGCCATTTCT CACAGAATTA AGACTGGAAA GGCCTCCATT 
CCTTTGAAGG AATTGGAGCA GTTTAACTCA GATATACAAA AATTGCTTGA ACCACTGGAG 
GCTGAAATTC AGCAGGGGGT GAATCTGAAA GAGGAAGACT TCAATAAAGA TATGAGTGAA 
GACAATGAGG GTACTGTAAA AGAATTGTTG CAAAGAGGAG ACAACTTACA ACAAAGAATC 
ACAGATGAGA GAAAGCGAGA GGAAATAAAG ATAAAACAAC AGCTGTTACA GACAAAACAT 
AATGCTCTCA AGGATTTGAG GTCTCAAAGA AGAAAAAAGG CTCTAGAAAT TTCTCACCAG 
TGGTATCAGT ACAAGAGGCA GGCTGATGAT CTCCTGAAAT GCTTGGATGA CATTGAAAAA 
AAATTAGCCA GCCTACCTGA ACCCAGAGAT GAAAGGAAAA TAAAGGAAAT TGATCGTGAA 
TTGCAGAAGA AGAAAGAGGA GCTGAATGCA GTGCGTAGGC AAGCTGAGGG CTTGTCTGAG 
GATGGGGCCG CAATGGCAGT GGAGCCAACT CAGATCCAGC TCAGCAAGCG CTGGCGGGAA 
ATTGAGAGCA AATTTGCTCA GTTTCGAAGA CTCAACTTTG CACAAATTCA CACTGTCCAT 
GAAGAGTCAG TGGTGGCGAT GACTGAAGAC ATGCCTTTGG AAATTTCTTA TGTGCCTTCT 
ACTTACCTGA CTGAGATCAC TCATGTCTCA CAAGCCCTAT CAGAAGTGGA AGAGCTTCTT 
AATGCTCCCG ACCTTTGTGC TCAAGATTTT GAAGATCTCT TTAAACAAGA GGAATCCTTG 
AAGAACATAA AAGACAGCCT GCAACAAATC TCAGGTCGGA TTGACATCAT TCACAATAAA 
AAGACAGCAG CATTGCACAG TGCCACTCCT GCAGAAAGGG CAAAGCTCCA GGAAGCTCTC 
TCACGGCTTG ATTTCCAATG GGAAAGAGTT AACAATATGT ACAAGGACCG ACAAGGGAGA 
TTTGACAGAT CTGTGGAAAA ATGGCGGCGG TTTCATTATG ATATGAAGAT ACTTAATCAA 
TGGCTAACAG AAGCTGAACA GTTTCTCAAA AAGACACAAA TTCCTGAGAA TTGGGAACAT 
GCCAAATACA AATGGTATCT TAAGGAACTC CAGGATGGCA TTGGACAGCG GCAAAGTGTT 
GTCAGGGTAT TGAATGCAAC TGGGGAAGAA ATAATTCAAC AGTCCTCAAA AACAGATGCC 
AGTATTCTCC AAGAAAAACT GGGAAGCCTG AATCTGCGGT GGCAGGAGGT CTGCAAACAG 
CTGGCAGAAA GAAAAAAGAG GCTAGAGGAA CAGAAGAATA TCTTGTCAGA ATTTCAAAGA 
GATGTAAATG AATTTGTTTT ATGGTTGGAA GAAGCGGATA ACGTTGCTAA TATTCCACTT 
GAACCTGGAA ATGAGCAGCA GCTAAAAGAA AAACTTGAAC AAGTCAAGTT ACTGGCAGAA 
GAGTTGCCCC TGCGCCAGGG AATTCTAAAA CAATTAAATG AAACTGGAGG AACAGTGCTT 
GTAAGTGCTC CCCTAAGCCC AGAAGAGCAA GATAAACTTG AAAATAAGCT CAAGCAGACA 
AATCTTCAGT GGATAAAGGT TTCTAGAAAT CTGCCTGAGA AGCAAGAAGA AATTGAGGCA 
CACGTAAAAG ACCTTGGACA GCTGGAAGAG CAGTTAAATC ATCTGCTTCT ATGGCTGTCT 
CCTATTAGGA ATCAGTTGGA AATTTACAAT CAGCCAAATC AAACAGGACC ATTTGACATC 
AAGGAAATTG AAGTAGCAGT TCAAGCTAAA CAGCCGGATG TGGAAGGGAT TTTGTCTAAA 
GGGCAGCATT TGTACAAGGA AAAACCAGCC ACTCAGCCAG CGAAGAGAAA GCTGGAAGAT 
CTCAGCTCTG ATTGGAAGGT GGTAACTCAG TTGCTTCAAG AGCTGCGGGC AAAGCAGCCT 
GGCCCAGCTC CTGGACTGAC CACTGTCAGA GCCCCTCCCA GTCAGACTGT TACTCTGGTG 
ACACAACCCG CGGTTACCAA GGAAACTGCC ATCTCCAAAC TAGAAATGCC ATCTTCATTG 
CTGTTGGAGG TACCTGCACT GGCAGATTTC AACCGAGCTT GGACAGAACT TACCGACTGG 
CTGTCTCTGC TTGATCGAGT TATAAAATCA CAGAGGGTGA TGGTGGGTGA TCTTGAAGAC 
ATTAACGAGA TGATCATCAA GCAGAAGGCA ACGCTGCAGG ATTTGGAACA GAGGCGCCCC 
CAGTTGGAAG AACTCATTAC CGCTGCCCAG AATTTGAAAA ACAAGACCAG CAATCAAGAG 
GCTAGAACAA TCATTACTGA TCGAATTGAA AGAATTCAGA GTCAGTGGGA TGAAGTACAG 
GAACATCTTC AGAACCGGAG GCAACAGTTG AATGAAATGT TAAAGGATTC CACACAATGG 
CTGGAAGCTA AAGAGGAGGC TGAGCAGGTG TTGGGGCAGG CCAGAGCCAA GCTTGAGTCA 
TGGAAGGAGG CTCCCTACAC AGTAGATGCA ATCCAAAAGA AAATCACAGA AACCAAGCAG 
TTGGCCAAAG ACCTCCGCCA GTGGCAGATA AATGTAGATG TTGCAAATGA TTTGGCACTG 
AAACTTCTCC GAGATTATTC TGCAGATGAT ACCAGAAAAG TACACATGAT AACAGAGAAC 
ATCAATGCCT CTTGGGCAAG CATCCATAAA AGATTGAGTG AGCGAGAGGC TGCTCTGGAA 
GAAACCCACA GATTACTGCA ACAGTTCCCC TTGGACCTGG AGAAGTTCCT TGCCTGGCTT 
ACAGAAGCCG AAACAACTGC CAACGTCCTG CAGGATGCCA CCCATAAGGA AAGGCTTCTA 
GAAGATTCCA AGGGAGTAAG AGAGCTGATG AAACAATGGC AAGATCTCCA AGGAGAAATC 
GAAGCTCACA CAGATATCTA TCACAACCTG GACGAAAATG GCCAAAAAGT CCTGAGATCC 
CTGGAAGGTT CTGACGATGC AGCCTTGTTG CAAAGACGTT TGGATAACAT GAACTTCAAG 
TGGAGCGAAC TTCGGAAAAA GTCTCTCAAC ATTAGGTCTC ACTTGGAAGC CAGTTCTGAC 
CAGTGGAAGC GTCTGCACCT TTCTCTTCAG GAACTTCTGG TATGGCTCCA GCTGAAAGAT 
GATGAGTTAA GCCGGCAGGC ACCCATTGGA GGAGACTTTC CAGCGGTGCA GAAGCAGAAT 
GATGTACACA GGGCCTTCAA GAGGGAATTG AAAACGAAAG AACCTGTAAT CATGAGTACT 
CTTGAGACTG TACGAATATT TCTGACAGAG CAGCCTTTAG AAGGACTAGA GAAACTCTAC 
CAGGAGCCCA GAGAGCTGCC TCCTGAAGAG AGAGCCCAGA ATGTCACACG GCTCCTACGA 
AAGCAAGCTG AGGAGGTCAA CACTCAGTGG GAAAAACTGA ACGTGCACTC TGCAGACTGG 
CAGAGAAAAA TAGACGAGGC CCTCGAAAGA CTCCAGGAGC TTCAGGAAGC AACAGATGAG 
CTGGATCTCA AACTACGTCA GGCAGAGGTG ATCAAAGGAT CCTGGCAGCC TGTGGGTGAT 
CTCCTCATTG ACTCTCTCCA AGATCACCTC GAAAAAGTCA AGGCGCTTCG AGGAGAAATT 
ACACCTCTGA AAGAGAATGT CAGCTACGTC AATGACCTTG CTCGCCAACT CACTACGTTG 
GGCATTCAGC TGTCACCATA TAACCTCAAC ACTCTGGAAG ACCTGAACAC CAGATGGAAG 
CTTCTGCAGG TGGCCATTGA GGACCGCATC AGGCAGCTGC ATGAAGCGCA CAGGGACTTT 
GGACCAGCCT CCCAGCACTT CCTTTCCACT TCTGTCCAGG GTCCCTGGGA GAGAGCCATC 
TCACCAAACA AAGTGCCCTA CTATATCAAC CACGAGACCC AAACAACTTG CTGGGACCAT 
CCCAAAATGA CAGAGCTCTA CCAGTCTTTA GCTGACCTGA ATAATGTCAG GTTCTCAGCT 
TACAGGACTG CCATGAAACT CCGAAGACTG CAGAAGGCCC TTTGCTTGGA TCTCTTGAGC 
CTATCGGCTG CATGCGATGC CTTGGACCAG CACAACCTCA AGCAAAATGA CCAGCCCATG 
GATATCCTGC AGGTCATTAA CTGTCTGACC ACTATTTATG ATCGCCTAGA GCAAGAGCAC 
AACAATCTGG TCAACGTCCC TCTCTGCGTG GATATGTGTC TCAATTGGCT GCTGAATGTT 
TATGACACGG GACGAACGGG GAGGATCCGG GTCCTGTCTT TTAAAACTGG CATCATTTCT 
CTGTGTAAAG CCCATTTGGA AGACAAGTAC AGATACCTCT TCAAGCAAGT GGCAAGTTCG 
ACAGGATTTT GTGACCAGCG CAGGCTGGGC CTCCTCCTGC ATGACTCTAT CCAGATCCCA 
AGACAGTTGG GTGAAGTCGC ATCGTTCGGG GGCAGTAACA TTGAGCCGAG TGTCAGGAGC 
TGCTTCCAGT TTGCTAATAA TAAGCCTGAG ATCGAAGCGG CCCTCTTCCT AGACTGGATG 
CGCCTGGAGC CCCAGTCCAT GGTGTGGCTG CCTGTCCTGC ACCGAGTGGC TGCCGCGGAA 
ACTGCCAAGC ACCAGGCCAA GTGCAACATC TGCAAGGAGT GTCCCATCAT CGGATTCAGG 
TACAGGAGTC TAAAGCACTT TAATTATGAC ATCTGCCAAA GTTGCTTTTT TTCTGGTCGA 
GTTGCAAAAG GCCATAAAAT GCACTATCCC ATGGTGGAAT ACTGCACTCC GACTACATCG 
GGAGAAGATG TCCGTGACTT TGCCAAGGTA CTAAAAAACA AATTTCGAAC CAAAAGGTAT 
TTTGCGAAGC ATCCCCGAAT GGGCTACCTG CCAGTGCAGA CTGTCTTAGA GGGGGACAAC 
ATGGAAACAA ATCTGCAAGC AGAGTATGAT CGTCTAAAGC AGCAGCATGA ACACAAAGGC 
CTGTCCCCAC TGCCATCCCC TCCTGAAATG ATGCCTACTT CTCCCCAAAG TCCCCGGGAT 
GCTGAGCTCA TCGCTGAGGC CAAGCTGCTG CGTCAACACA AAGGCCGCCT GGAAGCCAGG 
ATGCAAATCC TTGAAGACCA TAACAAACAA CTGGAATCCC AGTTACACAG GCTCAGGCAG 
CTGCTGGAGC AACCCCAGGC AGAGGCCAAG GTGAATGGTA CAACGGTGTC TTCTCCTTCT 
ACCTCTCTTC AGAGGTCAGA TAGCAGTCAG CCTATGCTGC TCCGGGTGGT CGGCAGTCAG 
ACTTCAGAAT CCATGGGCGA GGAAGACCTG CTCAGCCCTC CCCAGGACAC AAGCACAGGG 
TTAGAGGAAG TGATGGAGCA GCTCAACCAC TCCTTCCCTA GTTCCAGAGG AAGAAATACC 
CCTGGGAAGC CAATGAGAGA GGACACAATG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMD ( XM_014111349.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013966824.1 Canis lupus familiaris dystrophin (DMD), transcript variant X11, mRNA.

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CloneID	OCe45420
Clone ID Related Accession (Same CDS sequence)	XM_014111349.1 , XM_014111349.2
Accession Version	XM_014111349.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10455bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	dystrophin isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATATG TTGCCACCAC TTATCCAGAT AAGAAGTCCA TCTTAATGTA TATCACATCA 
CTCTTCCAAG TTTTGCCTCA ACAAGTGAGC ATTGAAGCCA TCCAGGAAGT GGAAATGTTG 
CCAAGGCCAT CAAAAGTTAC TAGAGAAGAA CATTTTCAAT TACATCATCA AATGCACTAT 
TCTCAACAGA TCACAGTCAG TCTAGCACAG GGCTATGAAC GAGCCCCTTC CTCTCCTAAG 
CCTCGGTTCA AGAGCTATGC CTACACACAG GCTGCTTATG TCACCACTTC TGACCCCACA 
CGGAGCCCAC TTCCTTCACA GCATTTGGAA ACTCCTGAAG ACAAGTCATT TGGCCGGTCA 
TTGACAGAGA CCGAAGCAAA CCTGGACAGT TATCAAACAG CTTTGGAAGA AGTACTCTCG 
TGGCTTCTTT CAGCTGAGGA TGCACTGCAA GCCCAAGGAG AGATTTCTAA TGATGTCGAA 
GAAGTGAAAG AACAATTTCA TACTCATGAG GGATATATGA TGGACTTGAC ATCCCATCAG 
GGACGGGTCG GTAATGTTCT CCAACTGGGA AGTCAACTGA TTGGAACAGG GAAATTATCA 
GAAGATGAAG AAACCGAAGT GCAGGAACAA ATGAATCTCC TCAATTCAAG ATGGGAATGC 
CTCAGGGTAG CTAGCATGGA AAAACAAAGC AATTTACATA AAGTTCTAAT GGATCTCCAG 
AATCAGCAAC TGAAAGAGTT AAATGACTGG CTAACCAAAA CAGAAGAGAG AACAAGGAAA 
ATGGAGAAGG AGCCCCTTGG ACCTGATATT GAAGACCTAA AACGCCAAGT ACAACAACAT 
AAGGTGCTTC AAGAAGACTT AGAACAGGAA CAAGTCAGGG TCAATTCCCT CACTCATATG 
GTGGTGGTAG TCGATGAATC TAGTGGAGAC CATGCAACTG CTGCTTTGGA AGAACAACTT 
AAGGTACTGG GAGATCGATG GGCAAACATC TGTAGGTGGA CAGAAGATCG CTGGGTTCTT 
TTACAAGACA TCCTCCTAAA ATGGCAGCGT TTTACTGAAG AACAGTGCCT TTTCAGTGCA 
TGGCTTTCGG AGAAGGAAGA TGCAGTGAAC AAGATTCACA CAACTGGCTT TAAGGATCAA 
AGTGAAGTGT TATCAAATCT TCAGAAACTG GCTGTCTTAA AAACAGATCT GGAAAAGAAG 
AAGCAAACCA TGGACAAACT CTGCTCACTC AACCAAGACC TTCTTTCAGC GCTGAAAAAC 
ACAGTGGTAG CCCACAAGAT GGAAGCATGG CTGGACAACT TTGCCCAGCG CTGGGATAAT 
TTAGTCCAGA AACTTGAAAA AAGTTCAGCA CAGATTTCAC AGGCTGTCAC CACCACTCAG 
CCATCACTAA CACAGACAAC TGTAATGGAA ACAGTAACTA TGGTGACCAC GAGGGAACAC 
ATCTTGGTAA AGCATGCCCA AGAGGAACTG CCACCACCAC CCCCTCAGAA GAAGAGGCAG 
ATTATCGTGG ATTCTGAAAT TAGGAAAAGG TTGGATGTCG ATATAACTGA ACTTCACAGT 
TGGATTACTC GTTCAGAAGC TGTGTTGCAG AGTCCTGAAT TTGCAATCTA TCGGAAGGAA 
GGCAACTTCT CAGACCTTAA AGAAAAAGTC AATGCCATAG AGCGAGAAAA AGCCGAGAAG 
TTCAGAAAAC TGCAAGATGC CAGCAGATCA GCTCAGGCCC TGGTGGAACA GATGGTGAAT 
GAGGGTGTTA ATGCTGACAG CATCAAACAA GCCTCCGAAC AACTGAACAG CCGGTGGATA 
GAGTTCTGCC AATTGCTAAG CGAGAGACTT AACTGGCTGG AGTATCAGAA CAACATCATC 
ACTTTCTATA ATCAGCTACA ACAATTGGAG CAGATGACAA CTACTGCTGA AAACTGGTTG 
AAAACCCAGC CTACCACCAC ATCAGAGCCA ACAGCAATTA AAAGCCAGTT AAAAATTTGT 
AAGGATGAAA TCAACCGACT GTCAGCTCTT CAGCCTCAAA TCGAGCGATT AAAAATTCAA 
AGCATAGCCC TGAAAGAGAA AGGACAAGGG CCAATGTTCC TGGATGCAGA CTTTGTGGCC 
TTTACAAATC ATTTTAACCA AGTCTTTGCT GATGTGCAGG CAAGAGAAAA AGAGCTACAA 
ACAATTTTTG ACAGTTTGCC ACCCATGCGC TATCAGGAGA CTATGAGTAC CATCCTGACA 
TGGATCCAGC AGTCAGAAAC CAAACTCTCT ATACCTCAGG TTACTGTCAC TGAATATGAC 
ATCATGGAAC AGAGACTCGG AGAGCTACAG GCTTTACAAA GCTCTCTGCA AGAGCAACAA 
AATGGCCTAA ACTATCTCAG CACCACTGTG AAAGAGATGT CAAAGAAAGC ACCACTGTCT 
GATATTAGTC GGAAATATCA ATCAGAATTT GAAGAGATTG AGGGACGTTG GAAGAAGCTG 
TCTTCCCAGC TGGTTGAACA TTGTCAAAAG TTGGAGGAGC AAATGGCTAA ACTTCGAAAA 
ATTCAGAATC ACATAAAAAC TCTGAAGAAA TGGATCACTG AAGTCGATGT TTTCCTGAAG 
GAGGAATGGC CTGCCCTTGG GGATTCAGAA ATTCTGAAAA GACAGCTGAA ACAGTGCAGG 
CTTTTAGTCA ATGACATTCA GACCATCCAG CCTAGTCTCA ACAGTGTCAA TGAAGGGGCT 
CAGAAGATGA AGAATGAAGC AGAACCAGAG TTTGCTGGCA GACTTGAGAC AGAGCTCCGA 
GAACTTAACA CCCAGTGGGA TTACATGTGC CGCCAGGTCT ATGCCAGGAA GGAAGCCTTA 
AAAGGAGGTT TGGATAAAAC TGTAAGTCTT CAGAAAGATC TGTCAGAGAT GCATGAGTGG 
ATGACACAAG CTGAAGAAGA ATACCTAGAG AGAGATTTCG AATACAAGAC CCCTGATGAA 
TTACAGACAG CAGTTGAAGA GATGAAGAGA GCTAAAGAAG AGGCCCAGCA AAAAGAAGCA 
AAAGTGAAAC TCCTAACCGA GTCCGTCAAT AGTGTCATAG CTCAGGCTCC ACCTGCAGCA 
CAAGAGGCCT TAAAAAAGGA ACTTGACACT CTCACCACCA ACTACCAGTG GCTCTGCACC 
AGGCTCAATG GCAAATGCAA GACCTTGGAA GAAGTTTGGG CGTGCTGGCA TGAGTTATTG 
TCCTACTTGG AGAAGGCAAA CAAGTGGCTA AGTGAAGTAG AAGTCAAGCT TAAAACCACT 
GAAAATATTT CTGGGGGAGC TGAGGAAATC GCCGAGGTGC TTGATTCGCT TGAAAATTTG 
ATGCAACATT CAGAGGATAA CCCGAATCAG ATTCGCATAT TGGCACAGAC CTTGACAGAT 
GGTGGAGTCA TGGATGAACT GATCAATGAG GAGCTTGAGA CATTTAATTC TCGTTGGAGA 
GAACTCCATG AAGAGGCTGT GAGGAGGCAA AAGTTGCTTG AGCAGAGTAT CCAGTCGGCC 
CAGGAGATAG AAAAATCCTT GCACTTAATT CAGGAGTCCC TCTCTTCCAT TGACAAGCAG 
TTGGCAGCTT ATATTGCTGA CAAAGTGGAT GCAGCTCAGA TGCCTCAGGA AGCCCAGAAA 
ATCCAATCAG ATTTGACAAG TCATGAGATC AGTTTAGAAG AAATGAAGAA ACATAACCAG 
GGAAAGGAGA CTGCCCAAAG GGTACTATCC CAAATTGATG TGGCACAGAA AAAATTGCAG 
GATGTTTCCA TGAAGTTTCG ATTATTCCAG AAGCCAGCCA ATTTTGAGCA GCGCCTACAA 
GAAAGTAAAA TGATTTTAGA TGAAGTGAAG ATGCATTTAC CTGCGTTGGA AACAAAGAGT 
GTGGAACAGG AAGTAGTACA GTCACAGTTA AATCATTGTG TGAACTTGTA TAAAAGTCTG 
AGTGAAGTGA AGTCTGAAGT GGAAATGGTA ATAAAAACTG GACGTCAGAT TGTACAGAAG 
AAGCAGACGG AAAACCCGAA AGAGCTTGAT GAAAGAGTTA CAGCTTTGAA ATTGCATTAT 
AATGAGCTGG GAGCAAAGGT GACAGAAAGA AAGCAACAGT TGGAAAAATG CTTGAAATTG 
TCCCGTAAGA TGCGAAAGGA AATGAATGCC CTGACAGAAT GGCTGGCAGC GACAGATATG 
GAACTGACGA AGAGATCGGC AGTTGAAGGA ATGCCTAGTA ATTTGGATTC TGAAGTTGCC 
TGGGGAAAGG CTACTCAGAA AGAGATTGAG AAACAGAAGG TTCACCTAAA GAGTGTCACA 
GAGGTAGGAG AGGCCTTGAA AACGGTTTTG GGCAAGAAGG AAATGTTGGT GGAAGATAAA 
CTGAGTCTTC TGAATAGTAA CTGGATAGCC GTCACTTCCC GAGCAGAAGA GTGGTTAAAC 
CTTTTATTGG AATACCAGAA ACACATGGAA AACTTTGACC AGAATGTGGA TTACATCACA 
AACTGGATCA TTCAGGCTGA TGCACTTTTG GATGAATCTG AGAAAAAGAA ACCTCAGCAA 
AAAGAAGACA TACTTAAGCG TTTAAAGGCT GAAATGAATG ACATACGTCC AAAGGTGGAT 
TCTACACGTG ACCAAGCAGC AAACCTGATG GCAAACCGCG GCGACCACTG CAGGAAAGTA 
GTAGAGCCCA AAATCTCAGA GCTCAACCAT CGATTTGCAG CCATTTCTCA CAGAATTAAG 
ACTGGAAAGG CCTCCATTCC TTTGAAGGAA TTGGAGCAGT TTAACTCAGA TATACAAAAA 
TTGCTTGAAC CACTGGAGGC TGAAATTCAG CAGGGGGTGA ATCTGAAAGA GGAAGACTTC 
AATAAAGATA TGAGTGAAGA CAATGAGGGT ACTGTAAAAG AATTGTTGCA AAGAGGAGAC 
AACTTACAAC AAAGAATCAC AGATGAGAGA AAGCGAGAGG AAATAAAGAT AAAACAACAG 
CTGTTACAGA CAAAACATAA TGCTCTCAAG GATTTGAGGT CTCAAAGAAG AAAAAAGGCT 
CTAGAAATTT CTCACCAGTG GTATCAGTAC AAGAGGCAGG CTGATGATCT CCTGAAATGC 
TTGGATGACA TTGAAAAAAA ATTAGCCAGC CTACCTGAAC CCAGAGATGA AAGGAAAATA 
AAGGAAATTG ATCGTGAATT GCAGAAGAAG AAAGAGGAGC TGAATGCAGT GCGTAGGCAA 
GCTGAGGGCT TGTCTGAGGA TGGGGCCGCA ATGGCAGTGG AGCCAACTCA GATCCAGCTC 
AGCAAGCGCT GGCGGGAAAT TGAGAGCAAA TTTGCTCAGT TTCGAAGACT CAACTTTGCA 
CAAATTCACA CTGTCCATGA AGAGTCAGTG GTGGCGATGA CTGAAGACAT GCCTTTGGAA 
ATTTCTTATG TGCCTTCTAC TTACCTGACT GAGATCACTC ATGTCTCACA AGCCCTATCA 
GAAGTGGAAG AGCTTCTTAA TGCTCCCGAC CTTTGTGCTC AAGATTTTGA AGATCTCTTT 
AAACAAGAGG AATCCTTGAA GAACATAAAA GACAGCCTGC AACAAATCTC AGGTCGGATT 
GACATCATTC ACAATAAAAA GACAGCAGCA TTGCACAGTG CCACTCCTGC AGAAAGGGCA 
AAGCTCCAGG AAGCTCTCTC ACGGCTTGAT TTCCAATGGG AAAGAGTTAA CAATATGTAC 
AAGGACCGAC AAGGGAGATT TGACAGATCT GTGGAAAAAT GGCGGCGGTT TCATTATGAT 
ATGAAGATAC TTAATCAATG GCTAACAGAA GCTGAACAGT TTCTCAAAAA GACACAAATT 
CCTGAGAATT GGGAACATGC CAAATACAAA TGGTATCTTA AGGAACTCCA GGATGGCATT 
GGACAGCGGC AAAGTGTTGT CAGGGTATTG AATGCAACTG GGGAAGAAAT AATTCAACAG 
TCCTCAAAAA CAGATGCCAG TATTCTCCAA GAAAAACTGG GAAGCCTGAA TCTGCGGTGG 
CAGGAGGTCT GCAAACAGCT GGCAGAAAGA AAAAAGAGGC TAGAGGAACA GAAGAATATC 
TTGTCAGAAT TTCAAAGAGA TGTAAATGAA TTTGTTTTAT GGTTGGAAGA AGCGGATAAC 
GTTGCTAATA TTCCACTTGA ACCTGGAAAT GAGCAGCAGC TAAAAGAAAA ACTTGAACAA 
GTCAAGTTAC TGGCAGAAGA GTTGCCCCTG CGCCAGGGAA TTCTAAAACA ATTAAATGAA 
ACTGGAGGAA CAGTGCTTGT AAGTGCTCCC CTAAGCCCAG AAGAGCAAGA TAAACTTGAA 
AATAAGCTCA AGCAGACAAA TCTTCAGTGG ATAAAGGTTT CTAGAAATCT GCCTGAGAAG 
CAAGAAGAAA TTGAGGCACA CGTAAAAGAC CTTGGACAGC TGGAAGAGCA GTTAAATCAT 
CTGCTTCTAT GGCTGTCTCC TATTAGGAAT CAGTTGGAAA TTTACAATCA GCCAAATCAA 
ACAGGACCAT TTGACATCAA GGAAATTGAA GTAGCAGTTC AAGCTAAACA GCCGGATGTG 
GAAGGGATTT TGTCTAAAGG GCAGCATTTG TACAAGGAAA AACCAGCCAC TCAGCCAGCG 
AAGAGAAAGC TGGAAGATCT CAGCTCTGAT TGGAAGGTGG TAACTCAGTT GCTTCAAGAG 
CTGCGGGCAA AGCAGCCTGG CCCAGCTCCT GGACTGACCA CTGTCAGAGC CCCTCCCAGT 
CAGACTGTTA CTCTGGTGAC ACAACCCGCG GTTACCAAGG AAACTGCCAT CTCCAAACTA 
GAAATGCCAT CTTCATTGCT GTTGGAGGTA CCTGCACTGG CAGATTTCAA CCGAGCTTGG 
ACAGAACTTA CCGACTGGCT GTCTCTGCTT GATCGAGTTA TAAAATCACA GAGGGTGATG 
GTGGGTGATC TTGAAGACAT TAACGAGATG ATCATCAAGC AGAAGGCAAC GCTGCAGGAT 
TTGGAACAGA GGCGCCCCCA GTTGGAAGAA CTCATTACCG CTGCCCAGAA TTTGAAAAAC 
AAGACCAGCA ATCAAGAGGC TAGAACAATC ATTACTGATC GAATTGAAAG AATTCAGAGT 
CAGTGGGATG AAGTACAGGA ACATCTTCAG AACCGGAGGC AACAGTTGAA TGAAATGTTA 
AAGGATTCCA CACAATGGCT GGAAGCTAAA GAGGAGGCTG AGCAGGTGTT GGGGCAGGCC 
AGAGCCAAGC TTGAGTCATG GAAGGAGGCT CCCTACACAG TAGATGCAAT CCAAAAGAAA 
ATCACAGAAA CCAAGCAGTT GGCCAAAGAC CTCCGCCAGT GGCAGATAAA TGTAGATGTT 
GCAAATGATT TGGCACTGAA ACTTCTCCGA GATTATTCTG CAGATGATAC CAGAAAAGTA 
CACATGATAA CAGAGAACAT CAATGCCTCT TGGGCAAGCA TCCATAAAAG ATTGAGTGAG 
CGAGAGGCTG CTCTGGAAGA AACCCACAGA TTACTGCAAC AGTTCCCCTT GGACCTGGAG 
AAGTTCCTTG CCTGGCTTAC AGAAGCCGAA ACAACTGCCA ACGTCCTGCA GGATGCCACC 
CATAAGGAAA GGCTTCTAGA AGATTCCAAG GGAGTAAGAG AGCTGATGAA ACAATGGCAA 
GATCTCCAAG GAGAAATCGA AGCTCACACA GATATCTATC ACAACCTGGA CGAAAATGGC 
CAAAAAGTCC TGAGATCCCT GGAAGGTTCT GACGATGCAG CCTTGTTGCA AAGACGTTTG 
GATAACATGA ACTTCAAGTG GAGCGAACTT CGGAAAAAGT CTCTCAACAT TAGGTCTCAC 
TTGGAAGCCA GTTCTGACCA GTGGAAGCGT CTGCACCTTT CTCTTCAGGA ACTTCTGGTA 
TGGCTCCAGC TGAAAGATGA TGAGTTAAGC CGGCAGGCAC CCATTGGAGG AGACTTTCCA 
GCGGTGCAGA AGCAGAATGA TGTACACAGG GCCTTCAAGA GGGAATTGAA AACGAAAGAA 
CCTGTAATCA TGAGTACTCT TGAGACTGTA CGAATATTTC TGACAGAGCA GCCTTTAGAA 
GGACTAGAGA AACTCTACCA GGAGCCCAGA GAGCTGCCTC CTGAAGAGAG AGCCCAGAAT 
GTCACACGGC TCCTACGAAA GCAAGCTGAG GAGGTCAACA CTCAGTGGGA AAAACTGAAC 
GTGCACTCTG CAGACTGGCA GAGAAAAATA GACGAGGCCC TCGAAAGACT CCAGGAGCTT 
CAGGAAGCAA CAGATGAGCT GGATCTCAAA CTACGTCAGG CAGAGGTGAT CAAAGGATCC 
TGGCAGCCTG TGGGTGATCT CCTCATTGAC TCTCTCCAAG ATCACCTCGA AAAAGTCAAG 
GCGCTTCGAG GAGAAATTAC ACCTCTGAAA GAGAATGTCA GCTACGTCAA TGACCTTGCT 
CGCCAACTCA CTACGTTGGG CATTCAGCTG TCACCATATA ACCTCAACAC TCTGGAAGAC 
CTGAACACCA GATGGAAGCT TCTGCAGGTG GCCATTGAGG ACCGCATCAG GCAGCTGCAT 
GAAGCGCACA GGGACTTTGG ACCAGCCTCC CAGCACTTCC TTTCCACTTC TGTCCAGGGT 
CCCTGGGAGA GAGCCATCTC ACCAAACAAA GTGCCCTACT ATATCAACCA CGAGACCCAA 
ACAACTTGCT GGGACCATCC CAAAATGACA GAGCTCTACC AGTCTTTAGC TGACCTGAAT 
AATGTCAGGT TCTCAGCTTA CAGGACTGCC ATGAAACTCC GAAGACTGCA GAAGGCCCTT 
TGCTTGGATC TCTTGAGCCT ATCGGCTGCA TGCGATGCCT TGGACCAGCA CAACCTCAAG 
CAAAATGACC AGCCCATGGA TATCCTGCAG GTCATTAACT GTCTGACCAC TATTTATGAT 
CGCCTAGAGC AAGAGCACAA CAATCTGGTC AACGTCCCTC TCTGCGTGGA TATGTGTCTC 
AATTGGCTGC TGAATGTTTA TGACACGGGA CGAACGGGGA GGATCCGGGT CCTGTCTTTT 
AAAACTGGCA TCATTTCTCT GTGTAAAGCC CATTTGGAAG ACAAGTACAG ATACCTCTTC 
AAGCAAGTGG CAAGTTCGAC AGGATTTTGT GACCAGCGCA GGCTGGGCCT CCTCCTGCAT 
GACTCTATCC AGATCCCAAG ACAGTTGGGT GAAGTCGCAT CGTTCGGGGG CAGTAACATT 
GAGCCGAGTG TCAGGAGCTG CTTCCAGTTT GCTAATAATA AGCCTGAGAT CGAAGCGGCC 
CTCTTCCTAG ACTGGATGCG CCTGGAGCCC CAGTCCATGG TGTGGCTGCC TGTCCTGCAC 
CGAGTGGCTG CCGCGGAAAC TGCCAAGCAC CAGGCCAAGT GCAACATCTG CAAGGAGTGT 
CCCATCATCG GATTCAGGTA CAGGAGTCTA AAGCACTTTA ATTATGACAT CTGCCAAAGT 
TGCTTTTTTT CTGGTCGAGT TGCAAAAGGC CATAAAATGC ACTATCCCAT GGTGGAATAC 
TGCACTCCGA CTACATCGGG AGAAGATGTC CGTGACTTTG CCAAGGTACT AAAAAACAAA 
TTTCGAACCA AAAGGTATTT TGCGAAGCAT CCCCGAATGG GCTACCTGCC AGTGCAGACT 
GTCTTAGAGG GGGACAACAT GGAAACTCCT GTCACTCTGA TCAACTTCTG GCCGGTAGAT 
TCTGCGCCTG CCTCGTCCCC TCAGCTTTCA CACGATGATA CTCATTCACG CATTGAGCAT 
TATGCTAGCA GGCTAGCAGA AATGGAAAAC AGCAATGGAT CTTATCTAAA TGATAGCATC 
TCTCCTAATG AGAGCATAGA TGATGAACAT TTGTTAATCC AGCATTACTG CCAAAGTTTG 
AACCAGGACT CCCCCCTGAG CCAGCCTCGT AGTCCTGCCC AGATCTTGAT TTCCTTAGAG 
AGTGAGGAAA GAGGGGAGCT AGAGAGAATC CTAGCAGATC TTGAGGAAGA AAACAGAAAT 
CTGCAAGCAG AGTATGATCG TCTAAAGCAG CAGCATGAAC ACAAAGGCCT GTCCCCACTG 
CCATCCCCTC CTGAAATGAT GCCTACTTCT CCCCAAAGTC CCCGGGATGC TGAGCTCATC 
GCTGAGGCCA AGCTGCTGCG TCAACACAAA GGCCGCCTGG AAGCCAGGAT GCAAATCCTT 
GAAGACCATA ACAAACAACT GGAATCCCAG TTACACAGGC TCAGGCAGCT GCTGGAGCAA 
CCCCAGGCAG AGGCCAAGGT GAATGGTACA ACGGTGTCTT CTCCTTCTAC CTCTCTTCAG 
AGGTCAGATA GCAGTCAGCC TATGCTGCTC CGGGTGGTCG GCAGTCAGAC TTCAGAATCC 
ATGGGCGAGG AAGACCTGCT CAGCCCTCCC CAGGACACAA GCACAGGGTT AGAGGAAGTG 
ATGGAGCAGC TCAACCACTC CTTCCCTAGT TCCAGAGGAC ACAATGTAGG AAGCCTTTTC 
CACTTGGCAG ATGATTTGGG CAGAGCGATG GAGTCCTTAG TTTCAGTCAT GACAGATGAA 
GAAGGGGCAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013966824.1
CDS	78..10532
Translation	MEYVATTYPDKKSILMYITSLFQVLPQQVSIEAIQEVEMLPRPSKVTREEHFQLHHQMHYSQQITVSLAQGYERAPSSPKPRFKSYAYTQAAYVTTSDPTRSPLPSQHLETPEDKSFGRSLTETEANLDSYQTALEEVLSWLLSAEDALQAQGEISNDVEEVKEQFHTHEGYMMDLTSHQGRVGNVLQLGSQLIGTGKLSEDEETEVQEQMNLLNSRWECLRVASMEKQSNLHKVLMDLQNQQLKELNDWLTKTEERTRKMEKEPLGPDIEDLKRQVQQHKVLQEDLEQEQVRVNSLTHMVVVVDESSGDHATAALEEQLKVLGDRWANICRWTEDRWVLLQDILLKWQRFTEEQCLFSAWLSEKEDAVNKIHTTGFKDQSEVLSNLQKLAVLKTDLEKKKQTMDKLCSLNQDLLSALKNTVVAHKMEAWLDNFAQRWDNLVQKLEKSSAQISQAVTTTQPSLTQTTVMETVTMVTTREHILVKHAQEELPPPPPQKKRQIIVDSEIRKRLDVDITELHSWITRSEAVLQSPEFAIYRKEGNFSDLKEKVNAIEREKAEKFRKLQDASRSAQALVEQMVNEGVNADSIKQASEQLNSRWIEFCQLLSERLNWLEYQNNIITFYNQLQQLEQMTTTAENWLKTQPTTTSEPTAIKSQLKICKDEINRLSALQPQIERLKIQSIALKEKGQGPMFLDADFVAFTNHFNQVFADVQAREKELQTIFDSLPPMRYQETMSTILTWIQQSETKLSIPQVTVTEYDIMEQRLGELQALQSSLQEQQNGLNYLSTTVKEMSKKAPLSDISRKYQSEFEEIEGRWKKLSSQLVEHCQKLEEQMAKLRKIQNHIKTLKKWITEVDVFLKEEWPALGDSEILKRQLKQCRLLVNDIQTIQPSLNSVNEGAQKMKNEAEPEFAGRLETELRELNTQWDYMCRQVYARKEALKGGLDKTVSLQKDLSEMHEWMTQAEEEYLERDFEYKTPDELQTAVEEMKRAKEEAQQKEAKVKLLTESVNSVIAQAPPAAQEALKKELDTLTTNYQWLCTRLNGKCKTLEEVWACWHELLSYLEKANKWLSEVEVKLKTTENISGGAEEIAEVLDSLENLMQHSEDNPNQIRILAQTLTDGGVMDELINEELETFNSRWRELHEEAVRRQKLLEQSIQSAQEIEKSLHLIQESLSSIDKQLAAYIADKVDAAQMPQEAQKIQSDLTSHEISLEEMKKHNQGKETAQRVLSQIDVAQKKLQDVSMKFRLFQKPANFEQRLQESKMILDEVKMHLPALETKSVEQEVVQSQLNHCVNLYKSLSEVKSEVEMVIKTGRQIVQKKQTENPKELDERVTALKLHYNELGAKVTERKQQLEKCLKLSRKMRKEMNALTEWLAATDMELTKRSAVEGMPSNLDSEVAWGKATQKEIEKQKVHLKSVTEVGEALKTVLGKKEMLVEDKLSLLNSNWIAVTSRAEEWLNLLLEYQKHMENFDQNVDYITNWIIQADALLDESEKKKPQQKEDILKRLKAEMNDIRPKVDSTRDQAANLMANRGDHCRKVVEPKISELNHRFAAISHRIKTGKASIPLKELEQFNSDIQKLLEPLEAEIQQGVNLKEEDFNKDMSEDNEGTVKELLQRGDNLQQRITDERKREEIKIKQQLLQTKHNALKDLRSQRRKKALEISHQWYQYKRQADDLLKCLDDIEKKLASLPEPRDERKIKEIDRELQKKKEELNAVRRQAEGLSEDGAAMAVEPTQIQLSKRWREIESKFAQFRRLNFAQIHTVHEESVVAMTEDMPLEISYVPSTYLTEITHVSQALSEVEELLNAPDLCAQDFEDLFKQEESLKNIKDSLQQISGRIDIIHNKKTAALHSATPAERAKLQEALSRLDFQWERVNNMYKDRQGRFDRSVEKWRRFHYDMKILNQWLTEAEQFLKKTQIPENWEHAKYKWYLKELQDGIGQRQSVVRVLNATGEEIIQQSSKTDASILQEKLGSLNLRWQEVCKQLAERKKRLEEQKNILSEFQRDVNEFVLWLEEADNVANIPLEPGNEQQLKEKLEQVKLLAEELPLRQGILKQLNETGGTVLVSAPLSPEEQDKLENKLKQTNLQWIKVSRNLPEKQEEIEAHVKDLGQLEEQLNHLLLWLSPIRNQLEIYNQPNQTGPFDIKEIEVAVQAKQPDVEGILSKGQHLYKEKPATQPAKRKLEDLSSDWKVVTQLLQELRAKQPGPAPGLTTVRAPPSQTVTLVTQPAVTKETAISKLEMPSSLLLEVPALADFNRAWTELTDWLSLLDRVIKSQRVMVGDLEDINEMIIKQKATLQDLEQRRPQLEELITAAQNLKNKTSNQEARTIITDRIERIQSQWDEVQEHLQNRRQQLNEMLKDSTQWLEAKEEAEQVLGQARAKLESWKEAPYTVDAIQKKITETKQLAKDLRQWQINVDVANDLALKLLRDYSADDTRKVHMITENINASWASIHKRLSEREAALEETHRLLQQFPLDLEKFLAWLTEAETTANVLQDATHKERLLEDSKGVRELMKQWQDLQGEIEAHTDIYHNLDENGQKVLRSLEGSDDAALLQRRLDNMNFKWSELRKKSLNIRSHLEASSDQWKRLHLSLQELLVWLQLKDDELSRQAPIGGDFPAVQKQNDVHRAFKRELKTKEPVIMSTLETVRIFLTEQPLEGLEKLYQEPRELPPEERAQNVTRLLRKQAEEVNTQWEKLNVHSADWQRKIDEALERLQELQEATDELDLKLRQAEVIKGSWQPVGDLLIDSLQDHLEKVKALRGEITPLKENVSYVNDLARQLTTLGIQLSPYNLNTLEDLNTRWKLLQVAIEDRIRQLHEAHRDFGPASQHFLSTSVQGPWERAISPNKVPYYINHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQVINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPVTLINFWPVDSAPASSPQLSHDDTHSRIEHYASRLAEMENSNGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKQQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDTSTGLEEVMEQLNHSFPSSRGHNVGSLFHLADDLGRAMESLVSVMTDEEGAE

Target ORF information:

RefSeq Version	XM_014111349.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris dystrophin (DMD), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111349.1

ORF Insert Sequence:

ATGGAATATG TTGCCACCAC TTATCCAGAT AAGAAGTCCA TCTTAATGTA TATCACATCA 
CTCTTCCAAG TTTTGCCTCA ACAAGTGAGC ATTGAAGCCA TCCAGGAAGT GGAAATGTTG 
CCAAGGCCAT CAAAAGTTAC TAGAGAAGAA CATTTTCAAT TACATCATCA AATGCACTAT 
TCTCAACAGA TCACAGTCAG TCTAGCACAG GGCTATGAAC GAGCCCCTTC CTCTCCTAAG 
CCTCGGTTCA AGAGCTATGC CTACACACAG GCTGCTTATG TCACCACTTC TGACCCCACA 
CGGAGCCCAC TTCCTTCACA GCATTTGGAA ACTCCTGAAG ACAAGTCATT TGGCCGGTCA 
TTGACAGAGA CCGAAGCAAA CCTGGACAGT TATCAAACAG CTTTGGAAGA AGTACTCTCG 
TGGCTTCTTT CAGCTGAGGA TGCACTGCAA GCCCAAGGAG AGATTTCTAA TGATGTCGAA 
GAAGTGAAAG AACAATTTCA TACTCATGAG GGATATATGA TGGACTTGAC ATCCCATCAG 
GGACGGGTCG GTAATGTTCT CCAACTGGGA AGTCAACTGA TTGGAACAGG GAAATTATCA 
GAAGATGAAG AAACCGAAGT GCAGGAACAA ATGAATCTCC TCAATTCAAG ATGGGAATGC 
CTCAGGGTAG CTAGCATGGA AAAACAAAGC AATTTACATA AAGTTCTAAT GGATCTCCAG 
AATCAGCAAC TGAAAGAGTT AAATGACTGG CTAACCAAAA CAGAAGAGAG AACAAGGAAA 
ATGGAGAAGG AGCCCCTTGG ACCTGATATT GAAGACCTAA AACGCCAAGT ACAACAACAT 
AAGGTGCTTC AAGAAGACTT AGAACAGGAA CAAGTCAGGG TCAATTCCCT CACTCATATG 
GTGGTGGTAG TCGATGAATC TAGTGGAGAC CATGCAACTG CTGCTTTGGA AGAACAACTT 
AAGGTACTGG GAGATCGATG GGCAAACATC TGTAGGTGGA CAGAAGATCG CTGGGTTCTT 
TTACAAGACA TCCTCCTAAA ATGGCAGCGT TTTACTGAAG AACAGTGCCT TTTCAGTGCA 
TGGCTTTCGG AGAAGGAAGA TGCAGTGAAC AAGATTCACA CAACTGGCTT TAAGGATCAA 
AGTGAAGTGT TATCAAATCT TCAGAAACTG GCTGTCTTAA AAACAGATCT GGAAAAGAAG 
AAGCAAACCA TGGACAAACT CTGCTCACTC AACCAAGACC TTCTTTCAGC GCTGAAAAAC 
ACAGTGGTAG CCCACAAGAT GGAAGCATGG CTGGACAACT TTGCCCAGCG CTGGGATAAT 
TTAGTCCAGA AACTTGAAAA AAGTTCAGCA CAGATTTCAC AGGCTGTCAC CACCACTCAG 
CCATCACTAA CACAGACAAC TGTAATGGAA ACAGTAACTA TGGTGACCAC GAGGGAACAC 
ATCTTGGTAA AGCATGCCCA AGAGGAACTG CCACCACCAC CCCCTCAGAA GAAGAGGCAG 
ATTATCGTGG ATTCTGAAAT TAGGAAAAGG TTGGATGTCG ATATAACTGA ACTTCACAGT 
TGGATTACTC GTTCAGAAGC TGTGTTGCAG AGTCCTGAAT TTGCAATCTA TCGGAAGGAA 
GGCAACTTCT CAGACCTTAA AGAAAAAGTC AATGCCATAG AGCGAGAAAA AGCCGAGAAG 
TTCAGAAAAC TGCAAGATGC CAGCAGATCA GCTCAGGCCC TGGTGGAACA GATGGTGAAT 
GAGGGTGTTA ATGCTGACAG CATCAAACAA GCCTCCGAAC AACTGAACAG CCGGTGGATA 
GAGTTCTGCC AATTGCTAAG CGAGAGACTT AACTGGCTGG AGTATCAGAA CAACATCATC 
ACTTTCTATA ATCAGCTACA ACAATTGGAG CAGATGACAA CTACTGCTGA AAACTGGTTG 
AAAACCCAGC CTACCACCAC ATCAGAGCCA ACAGCAATTA AAAGCCAGTT AAAAATTTGT 
AAGGATGAAA TCAACCGACT GTCAGCTCTT CAGCCTCAAA TCGAGCGATT AAAAATTCAA 
AGCATAGCCC TGAAAGAGAA AGGACAAGGG CCAATGTTCC TGGATGCAGA CTTTGTGGCC 
TTTACAAATC ATTTTAACCA AGTCTTTGCT GATGTGCAGG CAAGAGAAAA AGAGCTACAA 
ACAATTTTTG ACAGTTTGCC ACCCATGCGC TATCAGGAGA CTATGAGTAC CATCCTGACA 
TGGATCCAGC AGTCAGAAAC CAAACTCTCT ATACCTCAGG TTACTGTCAC TGAATATGAC 
ATCATGGAAC AGAGACTCGG AGAGCTACAG GCTTTACAAA GCTCTCTGCA AGAGCAACAA 
AATGGCCTAA ACTATCTCAG CACCACTGTG AAAGAGATGT CAAAGAAAGC ACCACTGTCT 
GATATTAGTC GGAAATATCA ATCAGAATTT GAAGAGATTG AGGGACGTTG GAAGAAGCTG 
TCTTCCCAGC TGGTTGAACA TTGTCAAAAG TTGGAGGAGC AAATGGCTAA ACTTCGAAAA 
ATTCAGAATC ACATAAAAAC TCTGAAGAAA TGGATCACTG AAGTCGATGT TTTCCTGAAG 
GAGGAATGGC CTGCCCTTGG GGATTCAGAA ATTCTGAAAA GACAGCTGAA ACAGTGCAGG 
CTTTTAGTCA ATGACATTCA GACCATCCAG CCTAGTCTCA ACAGTGTCAA TGAAGGGGCT 
CAGAAGATGA AGAATGAAGC AGAACCAGAG TTTGCTGGCA GACTTGAGAC AGAGCTCCGA 
GAACTTAACA CCCAGTGGGA TTACATGTGC CGCCAGGTCT ATGCCAGGAA GGAAGCCTTA 
AAAGGAGGTT TGGATAAAAC TGTAAGTCTT CAGAAAGATC TGTCAGAGAT GCATGAGTGG 
ATGACACAAG CTGAAGAAGA ATACCTAGAG AGAGATTTCG AATACAAGAC CCCTGATGAA 
TTACAGACAG CAGTTGAAGA GATGAAGAGA GCTAAAGAAG AGGCCCAGCA AAAAGAAGCA 
AAAGTGAAAC TCCTAACCGA GTCCGTCAAT AGTGTCATAG CTCAGGCTCC ACCTGCAGCA 
CAAGAGGCCT TAAAAAAGGA ACTTGACACT CTCACCACCA ACTACCAGTG GCTCTGCACC 
AGGCTCAATG GCAAATGCAA GACCTTGGAA GAAGTTTGGG CGTGCTGGCA TGAGTTATTG 
TCCTACTTGG AGAAGGCAAA CAAGTGGCTA AGTGAAGTAG AAGTCAAGCT TAAAACCACT 
GAAAATATTT CTGGGGGAGC TGAGGAAATC GCCGAGGTGC TTGATTCGCT TGAAAATTTG 
ATGCAACATT CAGAGGATAA CCCGAATCAG ATTCGCATAT TGGCACAGAC CTTGACAGAT 
GGTGGAGTCA TGGATGAACT GATCAATGAG GAGCTTGAGA CATTTAATTC TCGTTGGAGA 
GAACTCCATG AAGAGGCTGT GAGGAGGCAA AAGTTGCTTG AGCAGAGTAT CCAGTCGGCC 
CAGGAGATAG AAAAATCCTT GCACTTAATT CAGGAGTCCC TCTCTTCCAT TGACAAGCAG 
TTGGCAGCTT ATATTGCTGA CAAAGTGGAT GCAGCTCAGA TGCCTCAGGA AGCCCAGAAA 
ATCCAATCAG ATTTGACAAG TCATGAGATC AGTTTAGAAG AAATGAAGAA ACATAACCAG 
GGAAAGGAGA CTGCCCAAAG GGTACTATCC CAAATTGATG TGGCACAGAA AAAATTGCAG 
GATGTTTCCA TGAAGTTTCG ATTATTCCAG AAGCCAGCCA ATTTTGAGCA GCGCCTACAA 
GAAAGTAAAA TGATTTTAGA TGAAGTGAAG ATGCATTTAC CTGCGTTGGA AACAAAGAGT 
GTGGAACAGG AAGTAGTACA GTCACAGTTA AATCATTGTG TGAACTTGTA TAAAAGTCTG 
AGTGAAGTGA AGTCTGAAGT GGAAATGGTA ATAAAAACTG GACGTCAGAT TGTACAGAAG 
AAGCAGACGG AAAACCCGAA AGAGCTTGAT GAAAGAGTTA CAGCTTTGAA ATTGCATTAT 
AATGAGCTGG GAGCAAAGGT GACAGAAAGA AAGCAACAGT TGGAAAAATG CTTGAAATTG 
TCCCGTAAGA TGCGAAAGGA AATGAATGCC CTGACAGAAT GGCTGGCAGC GACAGATATG 
GAACTGACGA AGAGATCGGC AGTTGAAGGA ATGCCTAGTA ATTTGGATTC TGAAGTTGCC 
TGGGGAAAGG CTACTCAGAA AGAGATTGAG AAACAGAAGG TTCACCTAAA GAGTGTCACA 
GAGGTAGGAG AGGCCTTGAA AACGGTTTTG GGCAAGAAGG AAATGTTGGT GGAAGATAAA 
CTGAGTCTTC TGAATAGTAA CTGGATAGCC GTCACTTCCC GAGCAGAAGA GTGGTTAAAC 
CTTTTATTGG AATACCAGAA ACACATGGAA AACTTTGACC AGAATGTGGA TTACATCACA 
AACTGGATCA TTCAGGCTGA TGCACTTTTG GATGAATCTG AGAAAAAGAA ACCTCAGCAA 
AAAGAAGACA TACTTAAGCG TTTAAAGGCT GAAATGAATG ACATACGTCC AAAGGTGGAT 
TCTACACGTG ACCAAGCAGC AAACCTGATG GCAAACCGCG GCGACCACTG CAGGAAAGTA 
GTAGAGCCCA AAATCTCAGA GCTCAACCAT CGATTTGCAG CCATTTCTCA CAGAATTAAG 
ACTGGAAAGG CCTCCATTCC TTTGAAGGAA TTGGAGCAGT TTAACTCAGA TATACAAAAA 
TTGCTTGAAC CACTGGAGGC TGAAATTCAG CAGGGGGTGA ATCTGAAAGA GGAAGACTTC 
AATAAAGATA TGAGTGAAGA CAATGAGGGT ACTGTAAAAG AATTGTTGCA AAGAGGAGAC 
AACTTACAAC AAAGAATCAC AGATGAGAGA AAGCGAGAGG AAATAAAGAT AAAACAACAG 
CTGTTACAGA CAAAACATAA TGCTCTCAAG GATTTGAGGT CTCAAAGAAG AAAAAAGGCT 
CTAGAAATTT CTCACCAGTG GTATCAGTAC AAGAGGCAGG CTGATGATCT CCTGAAATGC 
TTGGATGACA TTGAAAAAAA ATTAGCCAGC CTACCTGAAC CCAGAGATGA AAGGAAAATA 
AAGGAAATTG ATCGTGAATT GCAGAAGAAG AAAGAGGAGC TGAATGCAGT GCGTAGGCAA 
GCTGAGGGCT TGTCTGAGGA TGGGGCCGCA ATGGCAGTGG AGCCAACTCA GATCCAGCTC 
AGCAAGCGCT GGCGGGAAAT TGAGAGCAAA TTTGCTCAGT TTCGAAGACT CAACTTTGCA 
CAAATTCACA CTGTCCATGA AGAGTCAGTG GTGGCGATGA CTGAAGACAT GCCTTTGGAA 
ATTTCTTATG TGCCTTCTAC TTACCTGACT GAGATCACTC ATGTCTCACA AGCCCTATCA 
GAAGTGGAAG AGCTTCTTAA TGCTCCCGAC CTTTGTGCTC AAGATTTTGA AGATCTCTTT 
AAACAAGAGG AATCCTTGAA GAACATAAAA GACAGCCTGC AACAAATCTC AGGTCGGATT 
GACATCATTC ACAATAAAAA GACAGCAGCA TTGCACAGTG CCACTCCTGC AGAAAGGGCA 
AAGCTCCAGG AAGCTCTCTC ACGGCTTGAT TTCCAATGGG AAAGAGTTAA CAATATGTAC 
AAGGACCGAC AAGGGAGATT TGACAGATCT GTGGAAAAAT GGCGGCGGTT TCATTATGAT 
ATGAAGATAC TTAATCAATG GCTAACAGAA GCTGAACAGT TTCTCAAAAA GACACAAATT 
CCTGAGAATT GGGAACATGC CAAATACAAA TGGTATCTTA AGGAACTCCA GGATGGCATT 
GGACAGCGGC AAAGTGTTGT CAGGGTATTG AATGCAACTG GGGAAGAAAT AATTCAACAG 
TCCTCAAAAA CAGATGCCAG TATTCTCCAA GAAAAACTGG GAAGCCTGAA TCTGCGGTGG 
CAGGAGGTCT GCAAACAGCT GGCAGAAAGA AAAAAGAGGC TAGAGGAACA GAAGAATATC 
TTGTCAGAAT TTCAAAGAGA TGTAAATGAA TTTGTTTTAT GGTTGGAAGA AGCGGATAAC 
GTTGCTAATA TTCCACTTGA ACCTGGAAAT GAGCAGCAGC TAAAAGAAAA ACTTGAACAA 
GTCAAGTTAC TGGCAGAAGA GTTGCCCCTG CGCCAGGGAA TTCTAAAACA ATTAAATGAA 
ACTGGAGGAA CAGTGCTTGT AAGTGCTCCC CTAAGCCCAG AAGAGCAAGA TAAACTTGAA 
AATAAGCTCA AGCAGACAAA TCTTCAGTGG ATAAAGGTTT CTAGAAATCT GCCTGAGAAG 
CAAGAAGAAA TTGAGGCACA CGTAAAAGAC CTTGGACAGC TGGAAGAGCA GTTAAATCAT 
CTGCTTCTAT GGCTGTCTCC TATTAGGAAT CAGTTGGAAA TTTACAATCA GCCAAATCAA 
ACAGGACCAT TTGACATCAA GGAAATTGAA GTAGCAGTTC AAGCTAAACA GCCGGATGTG 
GAAGGGATTT TGTCTAAAGG GCAGCATTTG TACAAGGAAA AACCAGCCAC TCAGCCAGCG 
AAGAGAAAGC TGGAAGATCT CAGCTCTGAT TGGAAGGTGG TAACTCAGTT GCTTCAAGAG 
CTGCGGGCAA AGCAGCCTGG CCCAGCTCCT GGACTGACCA CTGTCAGAGC CCCTCCCAGT 
CAGACTGTTA CTCTGGTGAC ACAACCCGCG GTTACCAAGG AAACTGCCAT CTCCAAACTA 
GAAATGCCAT CTTCATTGCT GTTGGAGGTA CCTGCACTGG CAGATTTCAA CCGAGCTTGG 
ACAGAACTTA CCGACTGGCT GTCTCTGCTT GATCGAGTTA TAAAATCACA GAGGGTGATG 
GTGGGTGATC TTGAAGACAT TAACGAGATG ATCATCAAGC AGAAGGCAAC GCTGCAGGAT 
TTGGAACAGA GGCGCCCCCA GTTGGAAGAA CTCATTACCG CTGCCCAGAA TTTGAAAAAC 
AAGACCAGCA ATCAAGAGGC TAGAACAATC ATTACTGATC GAATTGAAAG AATTCAGAGT 
CAGTGGGATG AAGTACAGGA ACATCTTCAG AACCGGAGGC AACAGTTGAA TGAAATGTTA 
AAGGATTCCA CACAATGGCT GGAAGCTAAA GAGGAGGCTG AGCAGGTGTT GGGGCAGGCC 
AGAGCCAAGC TTGAGTCATG GAAGGAGGCT CCCTACACAG TAGATGCAAT CCAAAAGAAA 
ATCACAGAAA CCAAGCAGTT GGCCAAAGAC CTCCGCCAGT GGCAGATAAA TGTAGATGTT 
GCAAATGATT TGGCACTGAA ACTTCTCCGA GATTATTCTG CAGATGATAC CAGAAAAGTA 
CACATGATAA CAGAGAACAT CAATGCCTCT TGGGCAAGCA TCCATAAAAG ATTGAGTGAG 
CGAGAGGCTG CTCTGGAAGA AACCCACAGA TTACTGCAAC AGTTCCCCTT GGACCTGGAG 
AAGTTCCTTG CCTGGCTTAC AGAAGCCGAA ACAACTGCCA ACGTCCTGCA GGATGCCACC 
CATAAGGAAA GGCTTCTAGA AGATTCCAAG GGAGTAAGAG AGCTGATGAA ACAATGGCAA 
GATCTCCAAG GAGAAATCGA AGCTCACACA GATATCTATC ACAACCTGGA CGAAAATGGC 
CAAAAAGTCC TGAGATCCCT GGAAGGTTCT GACGATGCAG CCTTGTTGCA AAGACGTTTG 
GATAACATGA ACTTCAAGTG GAGCGAACTT CGGAAAAAGT CTCTCAACAT TAGGTCTCAC 
TTGGAAGCCA GTTCTGACCA GTGGAAGCGT CTGCACCTTT CTCTTCAGGA ACTTCTGGTA 
TGGCTCCAGC TGAAAGATGA TGAGTTAAGC CGGCAGGCAC CCATTGGAGG AGACTTTCCA 
GCGGTGCAGA AGCAGAATGA TGTACACAGG GCCTTCAAGA GGGAATTGAA AACGAAAGAA 
CCTGTAATCA TGAGTACTCT TGAGACTGTA CGAATATTTC TGACAGAGCA GCCTTTAGAA 
GGACTAGAGA AACTCTACCA GGAGCCCAGA GAGCTGCCTC CTGAAGAGAG AGCCCAGAAT 
GTCACACGGC TCCTACGAAA GCAAGCTGAG GAGGTCAACA CTCAGTGGGA AAAACTGAAC 
GTGCACTCTG CAGACTGGCA GAGAAAAATA GACGAGGCCC TCGAAAGACT CCAGGAGCTT 
CAGGAAGCAA CAGATGAGCT GGATCTCAAA CTACGTCAGG CAGAGGTGAT CAAAGGATCC 
TGGCAGCCTG TGGGTGATCT CCTCATTGAC TCTCTCCAAG ATCACCTCGA AAAAGTCAAG 
GCGCTTCGAG GAGAAATTAC ACCTCTGAAA GAGAATGTCA GCTACGTCAA TGACCTTGCT 
CGCCAACTCA CTACGTTGGG CATTCAGCTG TCACCATATA ACCTCAACAC TCTGGAAGAC 
CTGAACACCA GATGGAAGCT TCTGCAGGTG GCCATTGAGG ACCGCATCAG GCAGCTGCAT 
GAAGCGCACA GGGACTTTGG ACCAGCCTCC CAGCACTTCC TTTCCACTTC TGTCCAGGGT 
CCCTGGGAGA GAGCCATCTC ACCAAACAAA GTGCCCTACT ATATCAACCA CGAGACCCAA 
ACAACTTGCT GGGACCATCC CAAAATGACA GAGCTCTACC AGTCTTTAGC TGACCTGAAT 
AATGTCAGGT TCTCAGCTTA CAGGACTGCC ATGAAACTCC GAAGACTGCA GAAGGCCCTT 
TGCTTGGATC TCTTGAGCCT ATCGGCTGCA TGCGATGCCT TGGACCAGCA CAACCTCAAG 
CAAAATGACC AGCCCATGGA TATCCTGCAG GTCATTAACT GTCTGACCAC TATTTATGAT 
CGCCTAGAGC AAGAGCACAA CAATCTGGTC AACGTCCCTC TCTGCGTGGA TATGTGTCTC 
AATTGGCTGC TGAATGTTTA TGACACGGGA CGAACGGGGA GGATCCGGGT CCTGTCTTTT 
AAAACTGGCA TCATTTCTCT GTGTAAAGCC CATTTGGAAG ACAAGTACAG ATACCTCTTC 
AAGCAAGTGG CAAGTTCGAC AGGATTTTGT GACCAGCGCA GGCTGGGCCT CCTCCTGCAT 
GACTCTATCC AGATCCCAAG ACAGTTGGGT GAAGTCGCAT CGTTCGGGGG CAGTAACATT 
GAGCCGAGTG TCAGGAGCTG CTTCCAGTTT GCTAATAATA AGCCTGAGAT CGAAGCGGCC 
CTCTTCCTAG ACTGGATGCG CCTGGAGCCC CAGTCCATGG TGTGGCTGCC TGTCCTGCAC 
CGAGTGGCTG CCGCGGAAAC TGCCAAGCAC CAGGCCAAGT GCAACATCTG CAAGGAGTGT 
CCCATCATCG GATTCAGGTA CAGGAGTCTA AAGCACTTTA ATTATGACAT CTGCCAAAGT 
TGCTTTTTTT CTGGTCGAGT TGCAAAAGGC CATAAAATGC ACTATCCCAT GGTGGAATAC 
TGCACTCCGA CTACATCGGG AGAAGATGTC CGTGACTTTG CCAAGGTACT AAAAAACAAA 
TTTCGAACCA AAAGGTATTT TGCGAAGCAT CCCCGAATGG GCTACCTGCC AGTGCAGACT 
GTCTTAGAGG GGGACAACAT GGAAACTCCT GTCACTCTGA TCAACTTCTG GCCGGTAGAT 
TCTGCGCCTG CCTCGTCCCC TCAGCTTTCA CACGATGATA CTCATTCACG CATTGAGCAT 
TATGCTAGCA GGCTAGCAGA AATGGAAAAC AGCAATGGAT CTTATCTAAA TGATAGCATC 
TCTCCTAATG AGAGCATAGA TGATGAACAT TTGTTAATCC AGCATTACTG CCAAAGTTTG 
AACCAGGACT CCCCCCTGAG CCAGCCTCGT AGTCCTGCCC AGATCTTGAT TTCCTTAGAG 
AGTGAGGAAA GAGGGGAGCT AGAGAGAATC CTAGCAGATC TTGAGGAAGA AAACAGAAAT 
CTGCAAGCAG AGTATGATCG TCTAAAGCAG CAGCATGAAC ACAAAGGCCT GTCCCCACTG 
CCATCCCCTC CTGAAATGAT GCCTACTTCT CCCCAAAGTC CCCGGGATGC TGAGCTCATC 
GCTGAGGCCA AGCTGCTGCG TCAACACAAA GGCCGCCTGG AAGCCAGGAT GCAAATCCTT 
GAAGACCATA ACAAACAACT GGAATCCCAG TTACACAGGC TCAGGCAGCT GCTGGAGCAA 
CCCCAGGCAG AGGCCAAGGT GAATGGTACA ACGGTGTCTT CTCCTTCTAC CTCTCTTCAG 
AGGTCAGATA GCAGTCAGCC TATGCTGCTC CGGGTGGTCG GCAGTCAGAC TTCAGAATCC 
ATGGGCGAGG AAGACCTGCT CAGCCCTCCC CAGGACACAA GCACAGGGTT AGAGGAAGTG 
ATGGAGCAGC TCAACCACTC CTTCCCTAGT TCCAGAGGAC ACAATGTAGG AAGCCTTTTC 
CACTTGGCAG ATGATTTGGG CAGAGCGATG GAGTCCTTAG TTTCAGTCAT GACAGATGAA 
GAAGGGGCAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DMD ( XM_014111349.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013966824.1 Canis lupus familiaris dystrophin (DMD), transcript variant X10, mRNA.

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CloneID	OCe45420
Clone ID Related Accession (Same CDS sequence)	XM_014111349.1 , XM_014111349.2
Accession Version	XM_014111349.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10455bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	dystrophin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014111349.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATATG TTGCCACCAC TTATCCAGAT AAGAAGTCCA TCTTAATGTA TATCACATCA 
CTCTTCCAAG TTTTGCCTCA ACAAGTGAGC ATTGAAGCCA TCCAGGAAGT GGAAATGTTG 
CCAAGGCCAT CAAAAGTTAC TAGAGAAGAA CATTTTCAAT TACATCATCA AATGCACTAT 
TCTCAACAGA TCACAGTCAG TCTAGCACAG GGCTATGAAC GAGCCCCTTC CTCTCCTAAG 
CCTCGGTTCA AGAGCTATGC CTACACACAG GCTGCTTATG TCACCACTTC TGACCCCACA 
CGGAGCCCAC TTCCTTCACA GCATTTGGAA ACTCCTGAAG ACAAGTCATT TGGCCGGTCA 
TTGACAGAGA CCGAAGCAAA CCTGGACAGT TATCAAACAG CTTTGGAAGA AGTACTCTCG 
TGGCTTCTTT CAGCTGAGGA TGCACTGCAA GCCCAAGGAG AGATTTCTAA TGATGTCGAA 
GAAGTGAAAG AACAATTTCA TACTCATGAG GGATATATGA TGGACTTGAC ATCCCATCAG 
GGACGGGTCG GTAATGTTCT CCAACTGGGA AGTCAACTGA TTGGAACAGG GAAATTATCA 
GAAGATGAAG AAACCGAAGT GCAGGAACAA ATGAATCTCC TCAATTCAAG ATGGGAATGC 
CTCAGGGTAG CTAGCATGGA AAAACAAAGC AATTTACATA AAGTTCTAAT GGATCTCCAG 
AATCAGCAAC TGAAAGAGTT AAATGACTGG CTAACCAAAA CAGAAGAGAG AACAAGGAAA 
ATGGAGAAGG AGCCCCTTGG ACCTGATATT GAAGACCTAA AACGCCAAGT ACAACAACAT 
AAGGTGCTTC AAGAAGACTT AGAACAGGAA CAAGTCAGGG TCAATTCCCT CACTCATATG 
GTGGTGGTAG TCGATGAATC TAGTGGAGAC CATGCAACTG CTGCTTTGGA AGAACAACTT 
AAGGTACTGG GAGATCGATG GGCAAACATC TGTAGGTGGA CAGAAGATCG CTGGGTTCTT 
TTACAAGACA TCCTCCTAAA ATGGCAGCGT TTTACTGAAG AACAGTGCCT TTTCAGTGCA 
TGGCTTTCGG AGAAGGAAGA TGCAGTGAAC AAGATTCACA CAACTGGCTT TAAGGATCAA 
AGTGAAGTGT TATCAAATCT TCAGAAACTG GCTGTCTTAA AAACAGATCT GGAAAAGAAG 
AAGCAAACCA TGGACAAACT CTGCTCACTC AACCAAGACC TTCTTTCAGC GCTGAAAAAC 
ACAGTGGTAG CCCACAAGAT GGAAGCATGG CTGGACAACT TTGCCCAGCG CTGGGATAAT 
TTAGTCCAGA AACTTGAAAA AAGTTCAGCA CAGATTTCAC AGGCTGTCAC CACCACTCAG 
CCATCACTAA CACAGACAAC TGTAATGGAA ACAGTAACTA TGGTGACCAC GAGGGAACAC 
ATCTTGGTAA AGCATGCCCA AGAGGAACTG CCACCACCAC CCCCTCAGAA GAAGAGGCAG 
ATTATCGTGG ATTCTGAAAT TAGGAAAAGG TTGGATGTCG ATATAACTGA ACTTCACAGT 
TGGATTACTC GTTCAGAAGC TGTGTTGCAG AGTCCTGAAT TTGCAATCTA TCGGAAGGAA 
GGCAACTTCT CAGACCTTAA AGAAAAAGTC AATGCCATAG AGCGAGAAAA AGCCGAGAAG 
TTCAGAAAAC TGCAAGATGC CAGCAGATCA GCTCAGGCCC TGGTGGAACA GATGGTGAAT 
GAGGGTGTTA ATGCTGACAG CATCAAACAA GCCTCCGAAC AACTGAACAG CCGGTGGATA 
GAGTTCTGCC AATTGCTAAG CGAGAGACTT AACTGGCTGG AGTATCAGAA CAACATCATC 
ACTTTCTATA ATCAGCTACA ACAATTGGAG CAGATGACAA CTACTGCTGA AAACTGGTTG 
AAAACCCAGC CTACCACCAC ATCAGAGCCA ACAGCAATTA AAAGCCAGTT AAAAATTTGT 
AAGGATGAAA TCAACCGACT GTCAGCTCTT CAGCCTCAAA TCGAGCGATT AAAAATTCAA 
AGCATAGCCC TGAAAGAGAA AGGACAAGGG CCAATGTTCC TGGATGCAGA CTTTGTGGCC 
TTTACAAATC ATTTTAACCA AGTCTTTGCT GATGTGCAGG CAAGAGAAAA AGAGCTACAA 
ACAATTTTTG ACAGTTTGCC ACCCATGCGC TATCAGGAGA CTATGAGTAC CATCCTGACA 
TGGATCCAGC AGTCAGAAAC CAAACTCTCT ATACCTCAGG TTACTGTCAC TGAATATGAC 
ATCATGGAAC AGAGACTCGG AGAGCTACAG GCTTTACAAA GCTCTCTGCA AGAGCAACAA 
AATGGCCTAA ACTATCTCAG CACCACTGTG AAAGAGATGT CAAAGAAAGC ACCACTGTCT 
GATATTAGTC GGAAATATCA ATCAGAATTT GAAGAGATTG AGGGACGTTG GAAGAAGCTG 
TCTTCCCAGC TGGTTGAACA TTGTCAAAAG TTGGAGGAGC AAATGGCTAA ACTTCGAAAA 
ATTCAGAATC ACATAAAAAC TCTGAAGAAA TGGATCACTG AAGTCGATGT TTTCCTGAAG 
GAGGAATGGC CTGCCCTTGG GGATTCAGAA ATTCTGAAAA GACAGCTGAA ACAGTGCAGG 
CTTTTAGTCA ATGACATTCA GACCATCCAG CCTAGTCTCA ACAGTGTCAA TGAAGGGGCT 
CAGAAGATGA AGAATGAAGC AGAACCAGAG TTTGCTGGCA GACTTGAGAC AGAGCTCCGA 
GAACTTAACA CCCAGTGGGA TTACATGTGC CGCCAGGTCT ATGCCAGGAA GGAAGCCTTA 
AAAGGAGGTT TGGATAAAAC TGTAAGTCTT CAGAAAGATC TGTCAGAGAT GCATGAGTGG 
ATGACACAAG CTGAAGAAGA ATACCTAGAG AGAGATTTCG AATACAAGAC CCCTGATGAA 
TTACAGACAG CAGTTGAAGA GATGAAGAGA GCTAAAGAAG AGGCCCAGCA AAAAGAAGCA 
AAAGTGAAAC TCCTAACCGA GTCCGTCAAT AGTGTCATAG CTCAGGCTCC ACCTGCAGCA 
CAAGAGGCCT TAAAAAAGGA ACTTGACACT CTCACCACCA ACTACCAGTG GCTCTGCACC 
AGGCTCAATG GCAAATGCAA GACCTTGGAA GAAGTTTGGG CGTGCTGGCA TGAGTTATTG 
TCCTACTTGG AGAAGGCAAA CAAGTGGCTA AGTGAAGTAG AAGTCAAGCT TAAAACCACT 
GAAAATATTT CTGGGGGAGC TGAGGAAATC GCCGAGGTGC TTGATTCGCT TGAAAATTTG 
ATGCAACATT CAGAGGATAA CCCGAATCAG ATTCGCATAT TGGCACAGAC CTTGACAGAT 
GGTGGAGTCA TGGATGAACT GATCAATGAG GAGCTTGAGA CATTTAATTC TCGTTGGAGA 
GAACTCCATG AAGAGGCTGT GAGGAGGCAA AAGTTGCTTG AGCAGAGTAT CCAGTCGGCC 
CAGGAGATAG AAAAATCCTT GCACTTAATT CAGGAGTCCC TCTCTTCCAT TGACAAGCAG 
TTGGCAGCTT ATATTGCTGA CAAAGTGGAT GCAGCTCAGA TGCCTCAGGA AGCCCAGAAA 
ATCCAATCAG ATTTGACAAG TCATGAGATC AGTTTAGAAG AAATGAAGAA ACATAACCAG 
GGAAAGGAGA CTGCCCAAAG GGTACTATCC CAAATTGATG TGGCACAGAA AAAATTGCAG 
GATGTTTCCA TGAAGTTTCG ATTATTCCAG AAGCCAGCCA ATTTTGAGCA GCGCCTACAA 
GAAAGTAAAA TGATTTTAGA TGAAGTGAAG ATGCATTTAC CTGCGTTGGA AACAAAGAGT 
GTGGAACAGG AAGTAGTACA GTCACAGTTA AATCATTGTG TGAACTTGTA TAAAAGTCTG 
AGTGAAGTGA AGTCTGAAGT GGAAATGGTA ATAAAAACTG GACGTCAGAT TGTACAGAAG 
AAGCAGACGG AAAACCCGAA AGAGCTTGAT GAAAGAGTTA CAGCTTTGAA ATTGCATTAT 
AATGAGCTGG GAGCAAAGGT GACAGAAAGA AAGCAACAGT TGGAAAAATG CTTGAAATTG 
TCCCGTAAGA TGCGAAAGGA AATGAATGCC CTGACAGAAT GGCTGGCAGC GACAGATATG 
GAACTGACGA AGAGATCGGC AGTTGAAGGA ATGCCTAGTA ATTTGGATTC TGAAGTTGCC 
TGGGGAAAGG CTACTCAGAA AGAGATTGAG AAACAGAAGG TTCACCTAAA GAGTGTCACA 
GAGGTAGGAG AGGCCTTGAA AACGGTTTTG GGCAAGAAGG AAATGTTGGT GGAAGATAAA 
CTGAGTCTTC TGAATAGTAA CTGGATAGCC GTCACTTCCC GAGCAGAAGA GTGGTTAAAC 
CTTTTATTGG AATACCAGAA ACACATGGAA AACTTTGACC AGAATGTGGA TTACATCACA 
AACTGGATCA TTCAGGCTGA TGCACTTTTG GATGAATCTG AGAAAAAGAA ACCTCAGCAA 
AAAGAAGACA TACTTAAGCG TTTAAAGGCT GAAATGAATG ACATACGTCC AAAGGTGGAT 
TCTACACGTG ACCAAGCAGC AAACCTGATG GCAAACCGCG GCGACCACTG CAGGAAAGTA 
GTAGAGCCCA AAATCTCAGA GCTCAACCAT CGATTTGCAG CCATTTCTCA CAGAATTAAG 
ACTGGAAAGG CCTCCATTCC TTTGAAGGAA TTGGAGCAGT TTAACTCAGA TATACAAAAA 
TTGCTTGAAC CACTGGAGGC TGAAATTCAG CAGGGGGTGA ATCTGAAAGA GGAAGACTTC 
AATAAAGATA TGAGTGAAGA CAATGAGGGT ACTGTAAAAG AATTGTTGCA AAGAGGAGAC 
AACTTACAAC AAAGAATCAC AGATGAGAGA AAGCGAGAGG AAATAAAGAT AAAACAACAG 
CTGTTACAGA CAAAACATAA TGCTCTCAAG GATTTGAGGT CTCAAAGAAG AAAAAAGGCT 
CTAGAAATTT CTCACCAGTG GTATCAGTAC AAGAGGCAGG CTGATGATCT CCTGAAATGC 
TTGGATGACA TTGAAAAAAA ATTAGCCAGC CTACCTGAAC CCAGAGATGA AAGGAAAATA 
AAGGAAATTG ATCGTGAATT GCAGAAGAAG AAAGAGGAGC TGAATGCAGT GCGTAGGCAA 
GCTGAGGGCT TGTCTGAGGA TGGGGCCGCA ATGGCAGTGG AGCCAACTCA GATCCAGCTC 
AGCAAGCGCT GGCGGGAAAT TGAGAGCAAA TTTGCTCAGT TTCGAAGACT CAACTTTGCA 
CAAATTCACA CTGTCCATGA AGAGTCAGTG GTGGCGATGA CTGAAGACAT GCCTTTGGAA 
ATTTCTTATG TGCCTTCTAC TTACCTGACT GAGATCACTC ATGTCTCACA AGCCCTATCA 
GAAGTGGAAG AGCTTCTTAA TGCTCCCGAC CTTTGTGCTC AAGATTTTGA AGATCTCTTT 
AAACAAGAGG AATCCTTGAA GAACATAAAA GACAGCCTGC AACAAATCTC AGGTCGGATT 
GACATCATTC ACAATAAAAA GACAGCAGCA TTGCACAGTG CCACTCCTGC AGAAAGGGCA 
AAGCTCCAGG AAGCTCTCTC ACGGCTTGAT TTCCAATGGG AAAGAGTTAA CAATATGTAC 
AAGGACCGAC AAGGGAGATT TGACAGATCT GTGGAAAAAT GGCGGCGGTT TCATTATGAT 
ATGAAGATAC TTAATCAATG GCTAACAGAA GCTGAACAGT TTCTCAAAAA GACACAAATT 
CCTGAGAATT GGGAACATGC CAAATACAAA TGGTATCTTA AGGAACTCCA GGATGGCATT 
GGACAGCGGC AAAGTGTTGT CAGGGTATTG AATGCAACTG GGGAAGAAAT AATTCAACAG 
TCCTCAAAAA CAGATGCCAG TATTCTCCAA GAAAAACTGG GAAGCCTGAA TCTGCGGTGG 
CAGGAGGTCT GCAAACAGCT GGCAGAAAGA AAAAAGAGGC TAGAGGAACA GAAGAATATC 
TTGTCAGAAT TTCAAAGAGA TGTAAATGAA TTTGTTTTAT GGTTGGAAGA AGCGGATAAC 
GTTGCTAATA TTCCACTTGA ACCTGGAAAT GAGCAGCAGC TAAAAGAAAA ACTTGAACAA 
GTCAAGTTAC TGGCAGAAGA GTTGCCCCTG CGCCAGGGAA TTCTAAAACA ATTAAATGAA 
ACTGGAGGAA CAGTGCTTGT AAGTGCTCCC CTAAGCCCAG AAGAGCAAGA TAAACTTGAA 
AATAAGCTCA AGCAGACAAA TCTTCAGTGG ATAAAGGTTT CTAGAAATCT GCCTGAGAAG 
CAAGAAGAAA TTGAGGCACA CGTAAAAGAC CTTGGACAGC TGGAAGAGCA GTTAAATCAT 
CTGCTTCTAT GGCTGTCTCC TATTAGGAAT CAGTTGGAAA TTTACAATCA GCCAAATCAA 
ACAGGACCAT TTGACATCAA GGAAATTGAA GTAGCAGTTC AAGCTAAACA GCCGGATGTG 
GAAGGGATTT TGTCTAAAGG GCAGCATTTG TACAAGGAAA AACCAGCCAC TCAGCCAGCG 
AAGAGAAAGC TGGAAGATCT CAGCTCTGAT TGGAAGGTGG TAACTCAGTT GCTTCAAGAG 
CTGCGGGCAA AGCAGCCTGG CCCAGCTCCT GGACTGACCA CTGTCAGAGC CCCTCCCAGT 
CAGACTGTTA CTCTGGTGAC ACAACCCGCG GTTACCAAGG AAACTGCCAT CTCCAAACTA 
GAAATGCCAT CTTCATTGCT GTTGGAGGTA CCTGCACTGG CAGATTTCAA CCGAGCTTGG 
ACAGAACTTA CCGACTGGCT GTCTCTGCTT GATCGAGTTA TAAAATCACA GAGGGTGATG 
GTGGGTGATC TTGAAGACAT TAACGAGATG ATCATCAAGC AGAAGGCAAC GCTGCAGGAT 
TTGGAACAGA GGCGCCCCCA GTTGGAAGAA CTCATTACCG CTGCCCAGAA TTTGAAAAAC 
AAGACCAGCA ATCAAGAGGC TAGAACAATC ATTACTGATC GAATTGAAAG AATTCAGAGT 
CAGTGGGATG AAGTACAGGA ACATCTTCAG AACCGGAGGC AACAGTTGAA TGAAATGTTA 
AAGGATTCCA CACAATGGCT GGAAGCTAAA GAGGAGGCTG AGCAGGTGTT GGGGCAGGCC 
AGAGCCAAGC TTGAGTCATG GAAGGAGGCT CCCTACACAG TAGATGCAAT CCAAAAGAAA 
ATCACAGAAA CCAAGCAGTT GGCCAAAGAC CTCCGCCAGT GGCAGATAAA TGTAGATGTT 
GCAAATGATT TGGCACTGAA ACTTCTCCGA GATTATTCTG CAGATGATAC CAGAAAAGTA 
CACATGATAA CAGAGAACAT CAATGCCTCT TGGGCAAGCA TCCATAAAAG ATTGAGTGAG 
CGAGAGGCTG CTCTGGAAGA AACCCACAGA TTACTGCAAC AGTTCCCCTT GGACCTGGAG 
AAGTTCCTTG CCTGGCTTAC AGAAGCCGAA ACAACTGCCA ACGTCCTGCA GGATGCCACC 
CATAAGGAAA GGCTTCTAGA AGATTCCAAG GGAGTAAGAG AGCTGATGAA ACAATGGCAA 
GATCTCCAAG GAGAAATCGA AGCTCACACA GATATCTATC ACAACCTGGA CGAAAATGGC 
CAAAAAGTCC TGAGATCCCT GGAAGGTTCT GACGATGCAG CCTTGTTGCA AAGACGTTTG 
GATAACATGA ACTTCAAGTG GAGCGAACTT CGGAAAAAGT CTCTCAACAT TAGGTCTCAC 
TTGGAAGCCA GTTCTGACCA GTGGAAGCGT CTGCACCTTT CTCTTCAGGA ACTTCTGGTA 
TGGCTCCAGC TGAAAGATGA TGAGTTAAGC CGGCAGGCAC CCATTGGAGG AGACTTTCCA 
GCGGTGCAGA AGCAGAATGA TGTACACAGG GCCTTCAAGA GGGAATTGAA AACGAAAGAA 
CCTGTAATCA TGAGTACTCT TGAGACTGTA CGAATATTTC TGACAGAGCA GCCTTTAGAA 
GGACTAGAGA AACTCTACCA GGAGCCCAGA GAGCTGCCTC CTGAAGAGAG AGCCCAGAAT 
GTCACACGGC TCCTACGAAA GCAAGCTGAG GAGGTCAACA CTCAGTGGGA AAAACTGAAC 
GTGCACTCTG CAGACTGGCA GAGAAAAATA GACGAGGCCC TCGAAAGACT CCAGGAGCTT 
CAGGAAGCAA CAGATGAGCT GGATCTCAAA CTACGTCAGG CAGAGGTGAT CAAAGGATCC 
TGGCAGCCTG TGGGTGATCT CCTCATTGAC TCTCTCCAAG ATCACCTCGA AAAAGTCAAG 
GCGCTTCGAG GAGAAATTAC ACCTCTGAAA GAGAATGTCA GCTACGTCAA TGACCTTGCT 
CGCCAACTCA CTACGTTGGG CATTCAGCTG TCACCATATA ACCTCAACAC TCTGGAAGAC 
CTGAACACCA GATGGAAGCT TCTGCAGGTG GCCATTGAGG ACCGCATCAG GCAGCTGCAT 
GAAGCGCACA GGGACTTTGG ACCAGCCTCC CAGCACTTCC TTTCCACTTC TGTCCAGGGT 
CCCTGGGAGA GAGCCATCTC ACCAAACAAA GTGCCCTACT ATATCAACCA CGAGACCCAA 
ACAACTTGCT GGGACCATCC CAAAATGACA GAGCTCTACC AGTCTTTAGC TGACCTGAAT 
AATGTCAGGT TCTCAGCTTA CAGGACTGCC ATGAAACTCC GAAGACTGCA GAAGGCCCTT 
TGCTTGGATC TCTTGAGCCT ATCGGCTGCA TGCGATGCCT TGGACCAGCA CAACCTCAAG 
CAAAATGACC AGCCCATGGA TATCCTGCAG GTCATTAACT GTCTGACCAC TATTTATGAT 
CGCCTAGAGC AAGAGCACAA CAATCTGGTC AACGTCCCTC TCTGCGTGGA TATGTGTCTC 
AATTGGCTGC TGAATGTTTA TGACACGGGA CGAACGGGGA GGATCCGGGT CCTGTCTTTT 
AAAACTGGCA TCATTTCTCT GTGTAAAGCC CATTTGGAAG ACAAGTACAG ATACCTCTTC 
AAGCAAGTGG CAAGTTCGAC AGGATTTTGT GACCAGCGCA GGCTGGGCCT CCTCCTGCAT 
GACTCTATCC AGATCCCAAG ACAGTTGGGT GAAGTCGCAT CGTTCGGGGG CAGTAACATT 
GAGCCGAGTG TCAGGAGCTG CTTCCAGTTT GCTAATAATA AGCCTGAGAT CGAAGCGGCC 
CTCTTCCTAG ACTGGATGCG CCTGGAGCCC CAGTCCATGG TGTGGCTGCC TGTCCTGCAC 
CGAGTGGCTG CCGCGGAAAC TGCCAAGCAC CAGGCCAAGT GCAACATCTG CAAGGAGTGT 
CCCATCATCG GATTCAGGTA CAGGAGTCTA AAGCACTTTA ATTATGACAT CTGCCAAAGT 
TGCTTTTTTT CTGGTCGAGT TGCAAAAGGC CATAAAATGC ACTATCCCAT GGTGGAATAC 
TGCACTCCGA CTACATCGGG AGAAGATGTC CGTGACTTTG CCAAGGTACT AAAAAACAAA 
TTTCGAACCA AAAGGTATTT TGCGAAGCAT CCCCGAATGG GCTACCTGCC AGTGCAGACT 
GTCTTAGAGG GGGACAACAT GGAAACTCCT GTCACTCTGA TCAACTTCTG GCCGGTAGAT 
TCTGCGCCTG CCTCGTCCCC TCAGCTTTCA CACGATGATA CTCATTCACG CATTGAGCAT 
TATGCTAGCA GGCTAGCAGA AATGGAAAAC AGCAATGGAT CTTATCTAAA TGATAGCATC 
TCTCCTAATG AGAGCATAGA TGATGAACAT TTGTTAATCC AGCATTACTG CCAAAGTTTG 
AACCAGGACT CCCCCCTGAG CCAGCCTCGT AGTCCTGCCC AGATCTTGAT TTCCTTAGAG 
AGTGAGGAAA GAGGGGAGCT AGAGAGAATC CTAGCAGATC TTGAGGAAGA AAACAGAAAT 
CTGCAAGCAG AGTATGATCG TCTAAAGCAG CAGCATGAAC ACAAAGGCCT GTCCCCACTG 
CCATCCCCTC CTGAAATGAT GCCTACTTCT CCCCAAAGTC CCCGGGATGC TGAGCTCATC 
GCTGAGGCCA AGCTGCTGCG TCAACACAAA GGCCGCCTGG AAGCCAGGAT GCAAATCCTT 
GAAGACCATA ACAAACAACT GGAATCCCAG TTACACAGGC TCAGGCAGCT GCTGGAGCAA 
CCCCAGGCAG AGGCCAAGGT GAATGGTACA ACGGTGTCTT CTCCTTCTAC CTCTCTTCAG 
AGGTCAGATA GCAGTCAGCC TATGCTGCTC CGGGTGGTCG GCAGTCAGAC TTCAGAATCC 
ATGGGCGAGG AAGACCTGCT CAGCCCTCCC CAGGACACAA GCACAGGGTT AGAGGAAGTG 
ATGGAGCAGC TCAACCACTC CTTCCCTAGT TCCAGAGGAC ACAATGTAGG AAGCCTTTTC 
CACTTGGCAG ATGATTTGGG CAGAGCGATG GAGTCCTTAG TTTCAGTCAT GACAGATGAA 
GAAGGGGCAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013966824.1
CDS	113..10567
Translation	MEYVATTYPDKKSILMYITSLFQVLPQQVSIEAIQEVEMLPRPSKVTREEHFQLHHQMHYSQQITVSLAQGYERAPSSPKPRFKSYAYTQAAYVTTSDPTRSPLPSQHLETPEDKSFGRSLTETEANLDSYQTALEEVLSWLLSAEDALQAQGEISNDVEEVKEQFHTHEGYMMDLTSHQGRVGNVLQLGSQLIGTGKLSEDEETEVQEQMNLLNSRWECLRVASMEKQSNLHKVLMDLQNQQLKELNDWLTKTEERTRKMEKEPLGPDIEDLKRQVQQHKVLQEDLEQEQVRVNSLTHMVVVVDESSGDHATAALEEQLKVLGDRWANICRWTEDRWVLLQDILLKWQRFTEEQCLFSAWLSEKEDAVNKIHTTGFKDQSEVLSNLQKLAVLKTDLEKKKQTMDKLCSLNQDLLSALKNTVVAHKMEAWLDNFAQRWDNLVQKLEKSSAQISQAVTTTQPSLTQTTVMETVTMVTTREHILVKHAQEELPPPPPQKKRQIIVDSEIRKRLDVDITELHSWITRSEAVLQSPEFAIYRKEGNFSDLKEKVNAIEREKAEKFRKLQDASRSAQALVEQMVNEGVNADSIKQASEQLNSRWIEFCQLLSERLNWLEYQNNIITFYNQLQQLEQMTTTAENWLKTQPTTTSEPTAIKSQLKICKDEINRLSALQPQIERLKIQSIALKEKGQGPMFLDADFVAFTNHFNQVFADVQAREKELQTIFDSLPPMRYQETMSTILTWIQQSETKLSIPQVTVTEYDIMEQRLGELQALQSSLQEQQNGLNYLSTTVKEMSKKAPLSDISRKYQSEFEEIEGRWKKLSSQLVEHCQKLEEQMAKLRKIQNHIKTLKKWITEVDVFLKEEWPALGDSEILKRQLKQCRLLVNDIQTIQPSLNSVNEGAQKMKNEAEPEFAGRLETELRELNTQWDYMCRQVYARKEALKGGLDKTVSLQKDLSEMHEWMTQAEEEYLERDFEYKTPDELQTAVEEMKRAKEEAQQKEAKVKLLTESVNSVIAQAPPAAQEALKKELDTLTTNYQWLCTRLNGKCKTLEEVWACWHELLSYLEKANKWLSEVEVKLKTTENISGGAEEIAEVLDSLENLMQHSEDNPNQIRILAQTLTDGGVMDELINEELETFNSRWRELHEEAVRRQKLLEQSIQSAQEIEKSLHLIQESLSSIDKQLAAYIADKVDAAQMPQEAQKIQSDLTSHEISLEEMKKHNQGKETAQRVLSQIDVAQKKLQDVSMKFRLFQKPANFEQRLQESKMILDEVKMHLPALETKSVEQEVVQSQLNHCVNLYKSLSEVKSEVEMVIKTGRQIVQKKQTENPKELDERVTALKLHYNELGAKVTERKQQLEKCLKLSRKMRKEMNALTEWLAATDMELTKRSAVEGMPSNLDSEVAWGKATQKEIEKQKVHLKSVTEVGEALKTVLGKKEMLVEDKLSLLNSNWIAVTSRAEEWLNLLLEYQKHMENFDQNVDYITNWIIQADALLDESEKKKPQQKEDILKRLKAEMNDIRPKVDSTRDQAANLMANRGDHCRKVVEPKISELNHRFAAISHRIKTGKASIPLKELEQFNSDIQKLLEPLEAEIQQGVNLKEEDFNKDMSEDNEGTVKELLQRGDNLQQRITDERKREEIKIKQQLLQTKHNALKDLRSQRRKKALEISHQWYQYKRQADDLLKCLDDIEKKLASLPEPRDERKIKEIDRELQKKKEELNAVRRQAEGLSEDGAAMAVEPTQIQLSKRWREIESKFAQFRRLNFAQIHTVHEESVVAMTEDMPLEISYVPSTYLTEITHVSQALSEVEELLNAPDLCAQDFEDLFKQEESLKNIKDSLQQISGRIDIIHNKKTAALHSATPAERAKLQEALSRLDFQWERVNNMYKDRQGRFDRSVEKWRRFHYDMKILNQWLTEAEQFLKKTQIPENWEHAKYKWYLKELQDGIGQRQSVVRVLNATGEEIIQQSSKTDASILQEKLGSLNLRWQEVCKQLAERKKRLEEQKNILSEFQRDVNEFVLWLEEADNVANIPLEPGNEQQLKEKLEQVKLLAEELPLRQGILKQLNETGGTVLVSAPLSPEEQDKLENKLKQTNLQWIKVSRNLPEKQEEIEAHVKDLGQLEEQLNHLLLWLSPIRNQLEIYNQPNQTGPFDIKEIEVAVQAKQPDVEGILSKGQHLYKEKPATQPAKRKLEDLSSDWKVVTQLLQELRAKQPGPAPGLTTVRAPPSQTVTLVTQPAVTKETAISKLEMPSSLLLEVPALADFNRAWTELTDWLSLLDRVIKSQRVMVGDLEDINEMIIKQKATLQDLEQRRPQLEELITAAQNLKNKTSNQEARTIITDRIERIQSQWDEVQEHLQNRRQQLNEMLKDSTQWLEAKEEAEQVLGQARAKLESWKEAPYTVDAIQKKITETKQLAKDLRQWQINVDVANDLALKLLRDYSADDTRKVHMITENINASWASIHKRLSEREAALEETHRLLQQFPLDLEKFLAWLTEAETTANVLQDATHKERLLEDSKGVRELMKQWQDLQGEIEAHTDIYHNLDENGQKVLRSLEGSDDAALLQRRLDNMNFKWSELRKKSLNIRSHLEASSDQWKRLHLSLQELLVWLQLKDDELSRQAPIGGDFPAVQKQNDVHRAFKRELKTKEPVIMSTLETVRIFLTEQPLEGLEKLYQEPRELPPEERAQNVTRLLRKQAEEVNTQWEKLNVHSADWQRKIDEALERLQELQEATDELDLKLRQAEVIKGSWQPVGDLLIDSLQDHLEKVKALRGEITPLKENVSYVNDLARQLTTLGIQLSPYNLNTLEDLNTRWKLLQVAIEDRIRQLHEAHRDFGPASQHFLSTSVQGPWERAISPNKVPYYINHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQVINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPVTLINFWPVDSAPASSPQLSHDDTHSRIEHYASRLAEMENSNGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKQQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDTSTGLEEVMEQLNHSFPSSRGHNVGSLFHLADDLGRAMESLVSVMTDEEGAE

Target ORF information:

RefSeq Version	XM_014111349.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris dystrophin (DMD), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111349.2

ORF Insert Sequence:

ATGGAATATG TTGCCACCAC TTATCCAGAT AAGAAGTCCA TCTTAATGTA TATCACATCA 
CTCTTCCAAG TTTTGCCTCA ACAAGTGAGC ATTGAAGCCA TCCAGGAAGT GGAAATGTTG 
CCAAGGCCAT CAAAAGTTAC TAGAGAAGAA CATTTTCAAT TACATCATCA AATGCACTAT 
TCTCAACAGA TCACAGTCAG TCTAGCACAG GGCTATGAAC GAGCCCCTTC CTCTCCTAAG 
CCTCGGTTCA AGAGCTATGC CTACACACAG GCTGCTTATG TCACCACTTC TGACCCCACA 
CGGAGCCCAC TTCCTTCACA GCATTTGGAA ACTCCTGAAG ACAAGTCATT TGGCCGGTCA 
TTGACAGAGA CCGAAGCAAA CCTGGACAGT TATCAAACAG CTTTGGAAGA AGTACTCTCG 
TGGCTTCTTT CAGCTGAGGA TGCACTGCAA GCCCAAGGAG AGATTTCTAA TGATGTCGAA 
GAAGTGAAAG AACAATTTCA TACTCATGAG GGATATATGA TGGACTTGAC ATCCCATCAG 
GGACGGGTCG GTAATGTTCT CCAACTGGGA AGTCAACTGA TTGGAACAGG GAAATTATCA 
GAAGATGAAG AAACCGAAGT GCAGGAACAA ATGAATCTCC TCAATTCAAG ATGGGAATGC 
CTCAGGGTAG CTAGCATGGA AAAACAAAGC AATTTACATA AAGTTCTAAT GGATCTCCAG 
AATCAGCAAC TGAAAGAGTT AAATGACTGG CTAACCAAAA CAGAAGAGAG AACAAGGAAA 
ATGGAGAAGG AGCCCCTTGG ACCTGATATT GAAGACCTAA AACGCCAAGT ACAACAACAT 
AAGGTGCTTC AAGAAGACTT AGAACAGGAA CAAGTCAGGG TCAATTCCCT CACTCATATG 
GTGGTGGTAG TCGATGAATC TAGTGGAGAC CATGCAACTG CTGCTTTGGA AGAACAACTT 
AAGGTACTGG GAGATCGATG GGCAAACATC TGTAGGTGGA CAGAAGATCG CTGGGTTCTT 
TTACAAGACA TCCTCCTAAA ATGGCAGCGT TTTACTGAAG AACAGTGCCT TTTCAGTGCA 
TGGCTTTCGG AGAAGGAAGA TGCAGTGAAC AAGATTCACA CAACTGGCTT TAAGGATCAA 
AGTGAAGTGT TATCAAATCT TCAGAAACTG GCTGTCTTAA AAACAGATCT GGAAAAGAAG 
AAGCAAACCA TGGACAAACT CTGCTCACTC AACCAAGACC TTCTTTCAGC GCTGAAAAAC 
ACAGTGGTAG CCCACAAGAT GGAAGCATGG CTGGACAACT TTGCCCAGCG CTGGGATAAT 
TTAGTCCAGA AACTTGAAAA AAGTTCAGCA CAGATTTCAC AGGCTGTCAC CACCACTCAG 
CCATCACTAA CACAGACAAC TGTAATGGAA ACAGTAACTA TGGTGACCAC GAGGGAACAC 
ATCTTGGTAA AGCATGCCCA AGAGGAACTG CCACCACCAC CCCCTCAGAA GAAGAGGCAG 
ATTATCGTGG ATTCTGAAAT TAGGAAAAGG TTGGATGTCG ATATAACTGA ACTTCACAGT 
TGGATTACTC GTTCAGAAGC TGTGTTGCAG AGTCCTGAAT TTGCAATCTA TCGGAAGGAA 
GGCAACTTCT CAGACCTTAA AGAAAAAGTC AATGCCATAG AGCGAGAAAA AGCCGAGAAG 
TTCAGAAAAC TGCAAGATGC CAGCAGATCA GCTCAGGCCC TGGTGGAACA GATGGTGAAT 
GAGGGTGTTA ATGCTGACAG CATCAAACAA GCCTCCGAAC AACTGAACAG CCGGTGGATA 
GAGTTCTGCC AATTGCTAAG CGAGAGACTT AACTGGCTGG AGTATCAGAA CAACATCATC 
ACTTTCTATA ATCAGCTACA ACAATTGGAG CAGATGACAA CTACTGCTGA AAACTGGTTG 
AAAACCCAGC CTACCACCAC ATCAGAGCCA ACAGCAATTA AAAGCCAGTT AAAAATTTGT 
AAGGATGAAA TCAACCGACT GTCAGCTCTT CAGCCTCAAA TCGAGCGATT AAAAATTCAA 
AGCATAGCCC TGAAAGAGAA AGGACAAGGG CCAATGTTCC TGGATGCAGA CTTTGTGGCC 
TTTACAAATC ATTTTAACCA AGTCTTTGCT GATGTGCAGG CAAGAGAAAA AGAGCTACAA 
ACAATTTTTG ACAGTTTGCC ACCCATGCGC TATCAGGAGA CTATGAGTAC CATCCTGACA 
TGGATCCAGC AGTCAGAAAC CAAACTCTCT ATACCTCAGG TTACTGTCAC TGAATATGAC 
ATCATGGAAC AGAGACTCGG AGAGCTACAG GCTTTACAAA GCTCTCTGCA AGAGCAACAA 
AATGGCCTAA ACTATCTCAG CACCACTGTG AAAGAGATGT CAAAGAAAGC ACCACTGTCT 
GATATTAGTC GGAAATATCA ATCAGAATTT GAAGAGATTG AGGGACGTTG GAAGAAGCTG 
TCTTCCCAGC TGGTTGAACA TTGTCAAAAG TTGGAGGAGC AAATGGCTAA ACTTCGAAAA 
ATTCAGAATC ACATAAAAAC TCTGAAGAAA TGGATCACTG AAGTCGATGT TTTCCTGAAG 
GAGGAATGGC CTGCCCTTGG GGATTCAGAA ATTCTGAAAA GACAGCTGAA ACAGTGCAGG 
CTTTTAGTCA ATGACATTCA GACCATCCAG CCTAGTCTCA ACAGTGTCAA TGAAGGGGCT 
CAGAAGATGA AGAATGAAGC AGAACCAGAG TTTGCTGGCA GACTTGAGAC AGAGCTCCGA 
GAACTTAACA CCCAGTGGGA TTACATGTGC CGCCAGGTCT ATGCCAGGAA GGAAGCCTTA 
AAAGGAGGTT TGGATAAAAC TGTAAGTCTT CAGAAAGATC TGTCAGAGAT GCATGAGTGG 
ATGACACAAG CTGAAGAAGA ATACCTAGAG AGAGATTTCG AATACAAGAC CCCTGATGAA 
TTACAGACAG CAGTTGAAGA GATGAAGAGA GCTAAAGAAG AGGCCCAGCA AAAAGAAGCA 
AAAGTGAAAC TCCTAACCGA GTCCGTCAAT AGTGTCATAG CTCAGGCTCC ACCTGCAGCA 
CAAGAGGCCT TAAAAAAGGA ACTTGACACT CTCACCACCA ACTACCAGTG GCTCTGCACC 
AGGCTCAATG GCAAATGCAA GACCTTGGAA GAAGTTTGGG CGTGCTGGCA TGAGTTATTG 
TCCTACTTGG AGAAGGCAAA CAAGTGGCTA AGTGAAGTAG AAGTCAAGCT TAAAACCACT 
GAAAATATTT CTGGGGGAGC TGAGGAAATC GCCGAGGTGC TTGATTCGCT TGAAAATTTG 
ATGCAACATT CAGAGGATAA CCCGAATCAG ATTCGCATAT TGGCACAGAC CTTGACAGAT 
GGTGGAGTCA TGGATGAACT GATCAATGAG GAGCTTGAGA CATTTAATTC TCGTTGGAGA 
GAACTCCATG AAGAGGCTGT GAGGAGGCAA AAGTTGCTTG AGCAGAGTAT CCAGTCGGCC 
CAGGAGATAG AAAAATCCTT GCACTTAATT CAGGAGTCCC TCTCTTCCAT TGACAAGCAG 
TTGGCAGCTT ATATTGCTGA CAAAGTGGAT GCAGCTCAGA TGCCTCAGGA AGCCCAGAAA 
ATCCAATCAG ATTTGACAAG TCATGAGATC AGTTTAGAAG AAATGAAGAA ACATAACCAG 
GGAAAGGAGA CTGCCCAAAG GGTACTATCC CAAATTGATG TGGCACAGAA AAAATTGCAG 
GATGTTTCCA TGAAGTTTCG ATTATTCCAG AAGCCAGCCA ATTTTGAGCA GCGCCTACAA 
GAAAGTAAAA TGATTTTAGA TGAAGTGAAG ATGCATTTAC CTGCGTTGGA AACAAAGAGT 
GTGGAACAGG AAGTAGTACA GTCACAGTTA AATCATTGTG TGAACTTGTA TAAAAGTCTG 
AGTGAAGTGA AGTCTGAAGT GGAAATGGTA ATAAAAACTG GACGTCAGAT TGTACAGAAG 
AAGCAGACGG AAAACCCGAA AGAGCTTGAT GAAAGAGTTA CAGCTTTGAA ATTGCATTAT 
AATGAGCTGG GAGCAAAGGT GACAGAAAGA AAGCAACAGT TGGAAAAATG CTTGAAATTG 
TCCCGTAAGA TGCGAAAGGA AATGAATGCC CTGACAGAAT GGCTGGCAGC GACAGATATG 
GAACTGACGA AGAGATCGGC AGTTGAAGGA ATGCCTAGTA ATTTGGATTC TGAAGTTGCC 
TGGGGAAAGG CTACTCAGAA AGAGATTGAG AAACAGAAGG TTCACCTAAA GAGTGTCACA 
GAGGTAGGAG AGGCCTTGAA AACGGTTTTG GGCAAGAAGG AAATGTTGGT GGAAGATAAA 
CTGAGTCTTC TGAATAGTAA CTGGATAGCC GTCACTTCCC GAGCAGAAGA GTGGTTAAAC 
CTTTTATTGG AATACCAGAA ACACATGGAA AACTTTGACC AGAATGTGGA TTACATCACA 
AACTGGATCA TTCAGGCTGA TGCACTTTTG GATGAATCTG AGAAAAAGAA ACCTCAGCAA 
AAAGAAGACA TACTTAAGCG TTTAAAGGCT GAAATGAATG ACATACGTCC AAAGGTGGAT 
TCTACACGTG ACCAAGCAGC AAACCTGATG GCAAACCGCG GCGACCACTG CAGGAAAGTA 
GTAGAGCCCA AAATCTCAGA GCTCAACCAT CGATTTGCAG CCATTTCTCA CAGAATTAAG 
ACTGGAAAGG CCTCCATTCC TTTGAAGGAA TTGGAGCAGT TTAACTCAGA TATACAAAAA 
TTGCTTGAAC CACTGGAGGC TGAAATTCAG CAGGGGGTGA ATCTGAAAGA GGAAGACTTC 
AATAAAGATA TGAGTGAAGA CAATGAGGGT ACTGTAAAAG AATTGTTGCA AAGAGGAGAC 
AACTTACAAC AAAGAATCAC AGATGAGAGA AAGCGAGAGG AAATAAAGAT AAAACAACAG 
CTGTTACAGA CAAAACATAA TGCTCTCAAG GATTTGAGGT CTCAAAGAAG AAAAAAGGCT 
CTAGAAATTT CTCACCAGTG GTATCAGTAC AAGAGGCAGG CTGATGATCT CCTGAAATGC 
TTGGATGACA TTGAAAAAAA ATTAGCCAGC CTACCTGAAC CCAGAGATGA AAGGAAAATA 
AAGGAAATTG ATCGTGAATT GCAGAAGAAG AAAGAGGAGC TGAATGCAGT GCGTAGGCAA 
GCTGAGGGCT TGTCTGAGGA TGGGGCCGCA ATGGCAGTGG AGCCAACTCA GATCCAGCTC 
AGCAAGCGCT GGCGGGAAAT TGAGAGCAAA TTTGCTCAGT TTCGAAGACT CAACTTTGCA 
CAAATTCACA CTGTCCATGA AGAGTCAGTG GTGGCGATGA CTGAAGACAT GCCTTTGGAA 
ATTTCTTATG TGCCTTCTAC TTACCTGACT GAGATCACTC ATGTCTCACA AGCCCTATCA 
GAAGTGGAAG AGCTTCTTAA TGCTCCCGAC CTTTGTGCTC AAGATTTTGA AGATCTCTTT 
AAACAAGAGG AATCCTTGAA GAACATAAAA GACAGCCTGC AACAAATCTC AGGTCGGATT 
GACATCATTC ACAATAAAAA GACAGCAGCA TTGCACAGTG CCACTCCTGC AGAAAGGGCA 
AAGCTCCAGG AAGCTCTCTC ACGGCTTGAT TTCCAATGGG AAAGAGTTAA CAATATGTAC 
AAGGACCGAC AAGGGAGATT TGACAGATCT GTGGAAAAAT GGCGGCGGTT TCATTATGAT 
ATGAAGATAC TTAATCAATG GCTAACAGAA GCTGAACAGT TTCTCAAAAA GACACAAATT 
CCTGAGAATT GGGAACATGC CAAATACAAA TGGTATCTTA AGGAACTCCA GGATGGCATT 
GGACAGCGGC AAAGTGTTGT CAGGGTATTG AATGCAACTG GGGAAGAAAT AATTCAACAG 
TCCTCAAAAA CAGATGCCAG TATTCTCCAA GAAAAACTGG GAAGCCTGAA TCTGCGGTGG 
CAGGAGGTCT GCAAACAGCT GGCAGAAAGA AAAAAGAGGC TAGAGGAACA GAAGAATATC 
TTGTCAGAAT TTCAAAGAGA TGTAAATGAA TTTGTTTTAT GGTTGGAAGA AGCGGATAAC 
GTTGCTAATA TTCCACTTGA ACCTGGAAAT GAGCAGCAGC TAAAAGAAAA ACTTGAACAA 
GTCAAGTTAC TGGCAGAAGA GTTGCCCCTG CGCCAGGGAA TTCTAAAACA ATTAAATGAA 
ACTGGAGGAA CAGTGCTTGT AAGTGCTCCC CTAAGCCCAG AAGAGCAAGA TAAACTTGAA 
AATAAGCTCA AGCAGACAAA TCTTCAGTGG ATAAAGGTTT CTAGAAATCT GCCTGAGAAG 
CAAGAAGAAA TTGAGGCACA CGTAAAAGAC CTTGGACAGC TGGAAGAGCA GTTAAATCAT 
CTGCTTCTAT GGCTGTCTCC TATTAGGAAT CAGTTGGAAA TTTACAATCA GCCAAATCAA 
ACAGGACCAT TTGACATCAA GGAAATTGAA GTAGCAGTTC AAGCTAAACA GCCGGATGTG 
GAAGGGATTT TGTCTAAAGG GCAGCATTTG TACAAGGAAA AACCAGCCAC TCAGCCAGCG 
AAGAGAAAGC TGGAAGATCT CAGCTCTGAT TGGAAGGTGG TAACTCAGTT GCTTCAAGAG 
CTGCGGGCAA AGCAGCCTGG CCCAGCTCCT GGACTGACCA CTGTCAGAGC CCCTCCCAGT 
CAGACTGTTA CTCTGGTGAC ACAACCCGCG GTTACCAAGG AAACTGCCAT CTCCAAACTA 
GAAATGCCAT CTTCATTGCT GTTGGAGGTA CCTGCACTGG CAGATTTCAA CCGAGCTTGG 
ACAGAACTTA CCGACTGGCT GTCTCTGCTT GATCGAGTTA TAAAATCACA GAGGGTGATG 
GTGGGTGATC TTGAAGACAT TAACGAGATG ATCATCAAGC AGAAGGCAAC GCTGCAGGAT 
TTGGAACAGA GGCGCCCCCA GTTGGAAGAA CTCATTACCG CTGCCCAGAA TTTGAAAAAC 
AAGACCAGCA ATCAAGAGGC TAGAACAATC ATTACTGATC GAATTGAAAG AATTCAGAGT 
CAGTGGGATG AAGTACAGGA ACATCTTCAG AACCGGAGGC AACAGTTGAA TGAAATGTTA 
AAGGATTCCA CACAATGGCT GGAAGCTAAA GAGGAGGCTG AGCAGGTGTT GGGGCAGGCC 
AGAGCCAAGC TTGAGTCATG GAAGGAGGCT CCCTACACAG TAGATGCAAT CCAAAAGAAA 
ATCACAGAAA CCAAGCAGTT GGCCAAAGAC CTCCGCCAGT GGCAGATAAA TGTAGATGTT 
GCAAATGATT TGGCACTGAA ACTTCTCCGA GATTATTCTG CAGATGATAC CAGAAAAGTA 
CACATGATAA CAGAGAACAT CAATGCCTCT TGGGCAAGCA TCCATAAAAG ATTGAGTGAG 
CGAGAGGCTG CTCTGGAAGA AACCCACAGA TTACTGCAAC AGTTCCCCTT GGACCTGGAG 
AAGTTCCTTG CCTGGCTTAC AGAAGCCGAA ACAACTGCCA ACGTCCTGCA GGATGCCACC 
CATAAGGAAA GGCTTCTAGA AGATTCCAAG GGAGTAAGAG AGCTGATGAA ACAATGGCAA 
GATCTCCAAG GAGAAATCGA AGCTCACACA GATATCTATC ACAACCTGGA CGAAAATGGC 
CAAAAAGTCC TGAGATCCCT GGAAGGTTCT GACGATGCAG CCTTGTTGCA AAGACGTTTG 
GATAACATGA ACTTCAAGTG GAGCGAACTT CGGAAAAAGT CTCTCAACAT TAGGTCTCAC 
TTGGAAGCCA GTTCTGACCA GTGGAAGCGT CTGCACCTTT CTCTTCAGGA ACTTCTGGTA 
TGGCTCCAGC TGAAAGATGA TGAGTTAAGC CGGCAGGCAC CCATTGGAGG AGACTTTCCA 
GCGGTGCAGA AGCAGAATGA TGTACACAGG GCCTTCAAGA GGGAATTGAA AACGAAAGAA 
CCTGTAATCA TGAGTACTCT TGAGACTGTA CGAATATTTC TGACAGAGCA GCCTTTAGAA 
GGACTAGAGA AACTCTACCA GGAGCCCAGA GAGCTGCCTC CTGAAGAGAG AGCCCAGAAT 
GTCACACGGC TCCTACGAAA GCAAGCTGAG GAGGTCAACA CTCAGTGGGA AAAACTGAAC 
GTGCACTCTG CAGACTGGCA GAGAAAAATA GACGAGGCCC TCGAAAGACT CCAGGAGCTT 
CAGGAAGCAA CAGATGAGCT GGATCTCAAA CTACGTCAGG CAGAGGTGAT CAAAGGATCC 
TGGCAGCCTG TGGGTGATCT CCTCATTGAC TCTCTCCAAG ATCACCTCGA AAAAGTCAAG 
GCGCTTCGAG GAGAAATTAC ACCTCTGAAA GAGAATGTCA GCTACGTCAA TGACCTTGCT 
CGCCAACTCA CTACGTTGGG CATTCAGCTG TCACCATATA ACCTCAACAC TCTGGAAGAC 
CTGAACACCA GATGGAAGCT TCTGCAGGTG GCCATTGAGG ACCGCATCAG GCAGCTGCAT 
GAAGCGCACA GGGACTTTGG ACCAGCCTCC CAGCACTTCC TTTCCACTTC TGTCCAGGGT 
CCCTGGGAGA GAGCCATCTC ACCAAACAAA GTGCCCTACT ATATCAACCA CGAGACCCAA 
ACAACTTGCT GGGACCATCC CAAAATGACA GAGCTCTACC AGTCTTTAGC TGACCTGAAT 
AATGTCAGGT TCTCAGCTTA CAGGACTGCC ATGAAACTCC GAAGACTGCA GAAGGCCCTT 
TGCTTGGATC TCTTGAGCCT ATCGGCTGCA TGCGATGCCT TGGACCAGCA CAACCTCAAG 
CAAAATGACC AGCCCATGGA TATCCTGCAG GTCATTAACT GTCTGACCAC TATTTATGAT 
CGCCTAGAGC AAGAGCACAA CAATCTGGTC AACGTCCCTC TCTGCGTGGA TATGTGTCTC 
AATTGGCTGC TGAATGTTTA TGACACGGGA CGAACGGGGA GGATCCGGGT CCTGTCTTTT 
AAAACTGGCA TCATTTCTCT GTGTAAAGCC CATTTGGAAG ACAAGTACAG ATACCTCTTC 
AAGCAAGTGG CAAGTTCGAC AGGATTTTGT GACCAGCGCA GGCTGGGCCT CCTCCTGCAT 
GACTCTATCC AGATCCCAAG ACAGTTGGGT GAAGTCGCAT CGTTCGGGGG CAGTAACATT 
GAGCCGAGTG TCAGGAGCTG CTTCCAGTTT GCTAATAATA AGCCTGAGAT CGAAGCGGCC 
CTCTTCCTAG ACTGGATGCG CCTGGAGCCC CAGTCCATGG TGTGGCTGCC TGTCCTGCAC 
CGAGTGGCTG CCGCGGAAAC TGCCAAGCAC CAGGCCAAGT GCAACATCTG CAAGGAGTGT 
CCCATCATCG GATTCAGGTA CAGGAGTCTA AAGCACTTTA ATTATGACAT CTGCCAAAGT 
TGCTTTTTTT CTGGTCGAGT TGCAAAAGGC CATAAAATGC ACTATCCCAT GGTGGAATAC 
TGCACTCCGA CTACATCGGG AGAAGATGTC CGTGACTTTG CCAAGGTACT AAAAAACAAA 
TTTCGAACCA AAAGGTATTT TGCGAAGCAT CCCCGAATGG GCTACCTGCC AGTGCAGACT 
GTCTTAGAGG GGGACAACAT GGAAACTCCT GTCACTCTGA TCAACTTCTG GCCGGTAGAT 
TCTGCGCCTG CCTCGTCCCC TCAGCTTTCA CACGATGATA CTCATTCACG CATTGAGCAT 
TATGCTAGCA GGCTAGCAGA AATGGAAAAC AGCAATGGAT CTTATCTAAA TGATAGCATC 
TCTCCTAATG AGAGCATAGA TGATGAACAT TTGTTAATCC AGCATTACTG CCAAAGTTTG 
AACCAGGACT CCCCCCTGAG CCAGCCTCGT AGTCCTGCCC AGATCTTGAT TTCCTTAGAG 
AGTGAGGAAA GAGGGGAGCT AGAGAGAATC CTAGCAGATC TTGAGGAAGA AAACAGAAAT 
CTGCAAGCAG AGTATGATCG TCTAAAGCAG CAGCATGAAC ACAAAGGCCT GTCCCCACTG 
CCATCCCCTC CTGAAATGAT GCCTACTTCT CCCCAAAGTC CCCGGGATGC TGAGCTCATC 
GCTGAGGCCA AGCTGCTGCG TCAACACAAA GGCCGCCTGG AAGCCAGGAT GCAAATCCTT 
GAAGACCATA ACAAACAACT GGAATCCCAG TTACACAGGC TCAGGCAGCT GCTGGAGCAA 
CCCCAGGCAG AGGCCAAGGT GAATGGTACA ACGGTGTCTT CTCCTTCTAC CTCTCTTCAG 
AGGTCAGATA GCAGTCAGCC TATGCTGCTC CGGGTGGTCG GCAGTCAGAC TTCAGAATCC 
ATGGGCGAGG AAGACCTGCT CAGCCCTCCC CAGGACACAA GCACAGGGTT AGAGGAAGTG 
ATGGAGCAGC TCAACCACTC CTTCCCTAGT TCCAGAGGAC ACAATGTAGG AAGCCTTTTC 
CACTTGGCAG ATGATTTGGG CAGAGCGATG GAGTCCTTAG TTTCAGTCAT GACAGATGAA 
GAAGGGGCAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Whole genome sequencing reveals a 7 base-pair deletion in DMD exon 42 in a dog with muscular dystrophy.
Mammalian genome : official journal of the International Mammalian Genome Society28(3-4)106-113(2017 Apr)
Nghiem PP,Bello L,Balog-Alvarez C,L?pez SM,Bettis A,Barnett H,Hernandez B,Schatzberg SJ,Piercy RJ,Kornegay JN

Dystrophin-deficient dogs with reduced myostatin have unequal muscle growth and greater joint contractures.
Skeletal muscle614(2016)
Kornegay JN,Bogan DJ,Bogan JR,Dow JL,Wang J,Fan Z,Liu N,Warsing LC,Grange RW,Ahn M,Balog-Alvarez CJ,Cotten SW,Willis MS,Brinkmeyer-Langford C,Zhu H,Palandra J,Morris CA,Styner MA,Wagner KR

Muscular dystrophy in the Japanese Spitz: an inversion disrupts the DMD and RPGR genes.
Animal genetics46(2)175-84(2015 Apr)
Atencia-Fernandez S,Shiel RE,Mooney CT,Nolan CM

Role of dystrophin in airway smooth muscle phenotype, contraction and lung function.
PloS one9(7)e102737(2014)
Sharma P,Basu S,Mitchell RW,Stelmack GL,Anderson JE,Halayko AJ

A comparative study of N-glycolylneuraminic acid (Neu5Gc) and cytotoxic T cell (CT) carbohydrate expression in normal and dystrophin-deficient dog and human skeletal muscle.
PloS one9(2)e88226(2014)
Martin PT,Golden B,Okerblom J,Camboni M,Chandrasekharan K,Xu R,Varki A,Flanigan KM,Kornegay JN

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DMD cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence: