GRIN2B cDNA ORF clone, Canis lupus familiaris(dog)

The following GRIN2B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GRIN2B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe26829 NM_001008719.1
Latest version!
Canis lupus familiaris glutamate ionotropic receptor NMDA type subunit 2B (GRIN2B), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OCe26829 XM_014108268.1
Latest version!
Canis lupus familiaris glutamate receptor, ionotropic, N-methyl D-aspartate 2B (GRIN2B), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OCe26829 XM_014108267.1
Latest version!
Canis lupus familiaris glutamate receptor, ionotropic, N-methyl D-aspartate 2B (GRIN2B), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OCe26829 XM_022410866.1
Latest version!
Canis lupus familiaris glutamate ionotropic receptor NMDA type subunit 2B (GRIN2B), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OCe26829 XM_005636716.2
Latest version!
Canis lupus familiaris glutamate receptor, ionotropic, N-methyl D-aspartate 2B (GRIN2B), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OCe26830 XM_022410868.1
Latest version!
Canis lupus familiaris glutamate ionotropic receptor NMDA type subunit 2B (GRIN2B), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OCe26830 XM_022410867.1
Latest version!
Canis lupus familiaris glutamate ionotropic receptor NMDA type subunit 2B (GRIN2B), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OCe43518 XM_014108270.1
Latest version!
Canis lupus familiaris glutamate receptor, ionotropic, N-methyl D-aspartate 2B (GRIN2B), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OCe43518 XM_014108269.1
Latest version!
Canis lupus familiaris glutamate receptor, ionotropic, N-methyl D-aspartate 2B (GRIN2B), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OCe26828 XM_022410865.1
Latest version!
Canis lupus familiaris glutamate ionotropic receptor NMDA type subunit 2B (GRIN2B), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe26829
    Clone ID Related Accession (Same CDS sequence) XM_022410866.1 , XM_014108268.1 , XM_005636716.2 , XM_014108267.1 , NM_001008719.1
    Accession Version NM_001008719.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4458bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-16
    Organism Canis lupus familiaris(dog)
    Product glutamate receptor ionotropic, NMDA 2B precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB195992.1. On Jan 18, 2005 this sequence version replaced XM_543802.1. ##Evidence-Data-START## Transcript exon combination :: AB195992.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA103936002, SAMEA2240594 [ECO:0000348] ##Evidence-Data-END##

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    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAGCCCA GAGCAGAGTG CTGTTCTCCC AAGTTCTGGT TGGTGTTGGC TGTCCTGGCT 
    GTGTCAGGCA GCAGAGCTCG TTCTCAGAAG AGCCCCCCCA GCATTGGCAT TGCTGTCATC
    CTCGTGGGCA CTTCAGACGA GGTGGCCATC AAGGATGCCC ACGAGAAAGA TGATTTCCAC
    CATCTCTCTG TGGTTCCCCG TGTGGAGCTG GTAGCCATGA ATGAGACTGA CCCAAAGAGC
    ATCATCACCC GCATCTGTGA TCTCATGTCT GACCGGAAGA TCCAGGGCGT GGTGTTTGCT
    GACGACACAG ATCAGGAGGC CATTGCCCAG ATCCTTGACT TCATTTCAGC CCAGACTCTC
    ACCCCCATCC TGGGCATCCA CGGAGGCTCC TCTATGATAA TGGCAGATAA GGATGAATCC
    TCCATGTTCT TCCAGTTTGG CCCATCAATT GAACAGCAAG CTTCTGTAAT GCTCAATATC
    ATGGAAGAGT ATGACTGGTA CATCTTTTCT ATTGTCACCA CCTACTTCCC TGGCTACCAG
    GACTTTGTAA ACAAGATTCG CAGCACCATT GAGAACAGCT TCGTGGGCTG GGAGTTAGAG
    GAAGTCCTCC TGCTGGACAT GTCCCTGGAT GATGGAGATT CTAAGATCCA GAACCAGCTC
    AAGAAACTGC AGAGCCCCAT CATTCTTCTC TATTGTACTA AGGAAGAAGC CACGTATATC
    TTTGAAGTAG CTAACTCAGT AGGGCTGACT GGCTACGGCT ACACATGGAT TGTACCTAGT
    CTGGTGGCAG GGGATACAGA CACAGTGCCC TCGGAGTTCC CCACTGGCCT CATTTCTGTG
    TCCTATGATG AATGGGACTA TGGCCTCCCT GCCAGAGTGA GGGATGGAAT TGCCATAATC
    ACCACTGCTG CTTCCGACAT GCTGTCTGAA CACAGCTTCA TCCCTGAGCC CAAGAGCAGT
    TGTTACAACA CCCATGAGAA GAGAATCTAC CAGTCCAACA TGCTAAATAG GTATCTGATC
    AATGTCACTT TTGAGGGAAG AAATCTGTCC TTCAGTGAAG ATGGCTACCA GATGCACCCG
    AAACTGGTGA TAATCCTTCT GAACAAGGAG AGAAAGTGGG AGAGGGTGGG GAAGTGGAAA
    GACAAATCCT TGCAGATGAA GTACTACGTG TGGCCCCGAA TGTGTCCTGA GACAGAAGAA
    CAGGAGGATG ACCATCTGAG CATTGTAACC CTGGAGGAGG CCCCCTTTGT CATTGTGGAG
    AGTGTGGACC CTCTGAGTGG AACCTGCATG AGGAACACAG TCCCCTGCCA AAAACGCATA
    GTCTCTGAGA ATAAAACAGA TGAAGAGCCA GGTTACATCA AAAAGTGCTG CAAGGGGTTC
    TGTATTGACA TCCTTAAGAA AATTTCTAAA TCTGTGAAGT TCACCTATGA TCTCTACCTG
    GTTACCAATG GCAAGCATGG GAAGAAAATC AATGGAACTT GGAATGGTAT GATTGGAGAG
    GTGGTTATGA AGAGGGCTTA CATGGCGGTG GGATCACTCA CCATCAATGA GGAGCGATCT
    GAGGTGGTTG ACTTCTCTGT GCCCTTCATA GAGACTGGCA TCAGTGTTAT GGTGTCACGC
    AGCAATGGGA CTGTCTCACC ATCTGCCTTC TTAGAGCCAT TCAGTGCTGA CGTATGGGTT
    ATGATGTTCG TGATGCTGCT CATCGTCTCA GCCGTGGCTG TCTTTGTCTT CGAGTACTTC
    AGCCCTGTGG GTTACAACAG GTGCCTCGCT GATGGCAGAG AGCCAGGTGG CCCGTCTTTT
    ACCATCGGCA AAGCTATTTG GTTACTGTGG GGTCTGGTGT TTAACAACTC CGTACCCGTG
    CAGAACCCAA AGGGGACCAC TTCCAAGATC ATGGTGTCGG TGTGGGCCTT CTTTGCCGTC
    ATCTTCCTGG CCAGCTACAC TGCCAACTTA GCTGCCTTCA TGATCCAAGA AGAGTATGTG
    GACCAGGTTT CTGGCCTGAG TGACAAAAAG TTCCAGAGAC CTAATGACTT CTCACCCCCT
    TTTCGCTTTG GGACTGTGCC CAATGGCAGC ACAGAGAGGA ATATTCGCAA TAACTACGCA
    GAAATGCATG CCTACATGGG AAAGTTCAAC CAGAGGGGTG TAGATGATGC ATTGCTCTCC
    CTGAAAACAG GGAAACTGGA TGCCTTCATC TATGATGCAG CAGTGCTGAA CTACATGGCG
    GGCAGAGATG AAGGCTGCAA GCTGGTGACC ATTGGCAGTG GGAAAGTCTT TGCTTCCACT
    GGTTATGGCA TTGCCATCCA AAAAGATTCT GGGTGGAAGC GCCAGGTGGA CCTTGCTATC
    CTCCAGCTAT TCGGAGATGG GGAGATGGAG GAGCTAGAAG CTCTTTGGCT CACTGGCATT
    TGCCACAATG AAAAGAATGA GGTCATGAGC AGCCAGCTGG ACATTGACAA CATGGCAGGA
    GTCTTCTACA TGTTGGGGGC AGCCATGGCT CTCAGTCTCA TCACCTTCAT CTGTGAACAC
    CTTTTCTATT GGCAGTTCCG GCACTGCTTT ATGGGTGTCT GTTCCGGCAA GCCTGGCATG
    GTCTTCTCCA TCAGCAGAGG TATCTACAGT TGCATCCATG GAGTGGCTAT CGAGGAGCGC
    CAGTCCGTGA TGAATTCCCC CACCGCGACC ATGAACAACA CACACTCCAA CATCCTGCGC
    CTGCTCCGCA CGGCCAAGAA CATGGCCAAC CTGTCTGGTG TGAACGGCTC CCCCCAGAGT
    GCCCTGGACT TCATCCGACG TGAGTCGTCC GTCTATGACA TCTCCGAGCA CCGCCGCAGC
    TTCACGCATT CCGACTGCAA ATCCTACAAC AACCCGCCGT GCGAGGAGAA CCTCTTCAGC
    GACTACATCA GTGAGGTGGA GAGGACTTTT GGTAACCTGC AGCTGAAGGA CAGCAACGTG
    TACCAAGATC ACTATCACCA CCACCACCGG CCCCACAGCA TCGGCAGCGC CAGCTCCATC
    GACGGGCTCT ACGACTGTGA CAACCCGCCT TTCACCACCC AGCCGAGGTC CATCAGCAAG
    AAGCCCCTGG ACCTCGGCCT CCCCTCCTCC AAACACAGCC AGCTCAGTGA CCTGTACGGC
    AAATTCTCCT TCAAGAGCGA CCGCTACAGT GGCCACGATG ACTTGATCCG CTCGGACGTG
    TCTGACATCT CCACGCACAC GGTCACCTAC GGAAACATAG AGGGCAACGC CGCCAAGAGG
    CGCAAGCAGC AATACAAGGA CAGCCTGAAG AAGCGGCCGG CCTCGGCCAA GTCCCGGCGG
    GAGTTCGACG AGATCGAGCT GGCCTACCGC CGCCGGCCGC CCCGCTCCCC TGACCACAAG
    CGCTACTTCA GGGACAAGGA GGGGCTCCGG GACTTCTACC TGGACCAGTT CCGCACCAAG
    GAGAACTCCC CGCACTGGGA GCACGTGGAC CTGACGGACA TCTACAAGGA GCAGAGCGAT
    GACTTTAAGC GCGACTCGGT GAGCGGAGGA GGGCCCTGTA CCAACAGGTC TCACCTCAAA
    CACGGGGCGG GCGACAAGCA CGGCGTGGTC AGCGGGGTCC CGGCCCCTTG GGAGAAGAAC
    CTGAGCAACG TGGATTGGGA GGACCGCTCC GGGGGCAACT TTTGCCGCAG CTGCCCCTCA
    AAGCTGCACA ACTACTCGAC GGCGGTGACC GGGCAGAACT CGGGCCGGCA GGCGTGCATC
    CGGTGCGAGG CCTGCAAGAA GGCAGGGAAC CTGTACGACA TCAGCGAGGA CAACTCCCTG
    CAGGAGCTGG ACCAGCCGGC TGCCCCTGTG GCGGTGCCGT CAAACGCGCC CAGCACCAAG
    TACCCCCAGA GCCCGACCAA CTCCAAGGCC CAGAAGAAGA CCCGGAACAA GCTGCGCCGG
    CAGCACTCCT ACGACACCTT CGTGGACCTG CAGAAGGAAG AAGCCGCCTT GGCCCCGCGC
    AGCGTGAGCC TGAAAGACAA GGGCCGGTTC CTGGACGGGA GTCCCTACGC CCACATGTTT
    GAGACGCCAG CTGGCGAGAG CACCTTTGCC AACCACGAGT CCTCAGTGGC CGCCGCCGGA
    CACCGCCACC ACAACAACCC CGGCGGCGGC GGCTACATGC TCAGCAAGTC GCTCTACCCC
    GACCGGGTCA CGCAAAACCC TTTCATCCCC ACTTTTGGGG ACGACCAGTG CTTGCTCCAT
    GGCAGCAAAT CCTACTTCTT CAGGCAGCCC ACGGTGGCGG GGGCGCCGAA AGCCAGGCCA
    GACTTCCGCG CCCTTGTCAC CAACAAGCCG GTGGTCTCGG CCCTGCACGG GGCCGTGCCA
    GGCCGTTTCC AGAAAGACAT CTGTATAGGG AACCAGTCCA ACCCCTGTGT GCCTAACAAC
    AAAAACCCCA GGGCTTTCAA TGGCTCCAGC AATGGGCATG TTTATGAGAA ACTTTCTAGT
    ATTGAGTCTG ATGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001008719.1
    CDS1..4458
    Translation

    Target ORF information:

    RefSeq Version NM_001008719.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris glutamate ionotropic receptor NMDA type subunit 2B (GRIN2B), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001008719.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAGCCCA GAGCAGAGTG CTGTTCTCCC AAGTTCTGGT TGGTGTTGGC TGTCCTGGCT 
    GTGTCAGGCA GCAGAGCTCG TTCTCAGAAG AGCCCCCCCA GCATTGGCAT TGCTGTCATC
    CTCGTGGGCA CTTCAGACGA GGTGGCCATC AAGGATGCCC ACGAGAAAGA TGATTTCCAC
    CATCTCTCTG TGGTTCCCCG TGTGGAGCTG GTAGCCATGA ATGAGACTGA CCCAAAGAGC
    ATCATCACCC GCATCTGTGA TCTCATGTCT GACCGGAAGA TCCAGGGCGT GGTGTTTGCT
    GACGACACAG ATCAGGAGGC CATTGCCCAG ATCCTTGACT TCATTTCAGC CCAGACTCTC
    ACCCCCATCC TGGGCATCCA CGGAGGCTCC TCTATGATAA TGGCAGATAA GGATGAATCC
    TCCATGTTCT TCCAGTTTGG CCCATCAATT GAACAGCAAG CTTCTGTAAT GCTCAATATC
    ATGGAAGAGT ATGACTGGTA CATCTTTTCT ATTGTCACCA CCTACTTCCC TGGCTACCAG
    GACTTTGTAA ACAAGATTCG CAGCACCATT GAGAACAGCT TCGTGGGCTG GGAGTTAGAG
    GAAGTCCTCC TGCTGGACAT GTCCCTGGAT GATGGAGATT CTAAGATCCA GAACCAGCTC
    AAGAAACTGC AGAGCCCCAT CATTCTTCTC TATTGTACTA AGGAAGAAGC CACGTATATC
    TTTGAAGTAG CTAACTCAGT AGGGCTGACT GGCTACGGCT ACACATGGAT TGTACCTAGT
    CTGGTGGCAG GGGATACAGA CACAGTGCCC TCGGAGTTCC CCACTGGCCT CATTTCTGTG
    TCCTATGATG AATGGGACTA TGGCCTCCCT GCCAGAGTGA GGGATGGAAT TGCCATAATC
    ACCACTGCTG CTTCCGACAT GCTGTCTGAA CACAGCTTCA TCCCTGAGCC CAAGAGCAGT
    TGTTACAACA CCCATGAGAA GAGAATCTAC CAGTCCAACA TGCTAAATAG GTATCTGATC
    AATGTCACTT TTGAGGGAAG AAATCTGTCC TTCAGTGAAG ATGGCTACCA GATGCACCCG
    AAACTGGTGA TAATCCTTCT GAACAAGGAG AGAAAGTGGG AGAGGGTGGG GAAGTGGAAA
    GACAAATCCT TGCAGATGAA GTACTACGTG TGGCCCCGAA TGTGTCCTGA GACAGAAGAA
    CAGGAGGATG ACCATCTGAG CATTGTAACC CTGGAGGAGG CCCCCTTTGT CATTGTGGAG
    AGTGTGGACC CTCTGAGTGG AACCTGCATG AGGAACACAG TCCCCTGCCA AAAACGCATA
    GTCTCTGAGA ATAAAACAGA TGAAGAGCCA GGTTACATCA AAAAGTGCTG CAAGGGGTTC
    TGTATTGACA TCCTTAAGAA AATTTCTAAA TCTGTGAAGT TCACCTATGA TCTCTACCTG
    GTTACCAATG GCAAGCATGG GAAGAAAATC AATGGAACTT GGAATGGTAT GATTGGAGAG
    GTGGTTATGA AGAGGGCTTA CATGGCGGTG GGATCACTCA CCATCAATGA GGAGCGATCT
    GAGGTGGTTG ACTTCTCTGT GCCCTTCATA GAGACTGGCA TCAGTGTTAT GGTGTCACGC
    AGCAATGGGA CTGTCTCACC ATCTGCCTTC TTAGAGCCAT TCAGTGCTGA CGTATGGGTT
    ATGATGTTCG TGATGCTGCT CATCGTCTCA GCCGTGGCTG TCTTTGTCTT CGAGTACTTC
    AGCCCTGTGG GTTACAACAG GTGCCTCGCT GATGGCAGAG AGCCAGGTGG CCCGTCTTTT
    ACCATCGGCA AAGCTATTTG GTTACTGTGG GGTCTGGTGT TTAACAACTC CGTACCCGTG
    CAGAACCCAA AGGGGACCAC TTCCAAGATC ATGGTGTCGG TGTGGGCCTT CTTTGCCGTC
    ATCTTCCTGG CCAGCTACAC TGCCAACTTA GCTGCCTTCA TGATCCAAGA AGAGTATGTG
    GACCAGGTTT CTGGCCTGAG TGACAAAAAG TTCCAGAGAC CTAATGACTT CTCACCCCCT
    TTTCGCTTTG GGACTGTGCC CAATGGCAGC ACAGAGAGGA ATATTCGCAA TAACTACGCA
    GAAATGCATG CCTACATGGG AAAGTTCAAC CAGAGGGGTG TAGATGATGC ATTGCTCTCC
    CTGAAAACAG GGAAACTGGA TGCCTTCATC TATGATGCAG CAGTGCTGAA CTACATGGCG
    GGCAGAGATG AAGGCTGCAA GCTGGTGACC ATTGGCAGTG GGAAAGTCTT TGCTTCCACT
    GGTTATGGCA TTGCCATCCA AAAAGATTCT GGGTGGAAGC GCCAGGTGGA CCTTGCTATC
    CTCCAGCTAT TCGGAGATGG GGAGATGGAG GAGCTAGAAG CTCTTTGGCT CACTGGCATT
    TGCCACAATG AAAAGAATGA GGTCATGAGC AGCCAGCTGG ACATTGACAA CATGGCAGGA
    GTCTTCTACA TGTTGGGGGC AGCCATGGCT CTCAGTCTCA TCACCTTCAT CTGTGAACAC
    CTTTTCTATT GGCAGTTCCG GCACTGCTTT ATGGGTGTCT GTTCCGGCAA GCCTGGCATG
    GTCTTCTCCA TCAGCAGAGG TATCTACAGT TGCATCCATG GAGTGGCTAT CGAGGAGCGC
    CAGTCCGTGA TGAATTCCCC CACCGCGACC ATGAACAACA CACACTCCAA CATCCTGCGC
    CTGCTCCGCA CGGCCAAGAA CATGGCCAAC CTGTCTGGTG TGAACGGCTC CCCCCAGAGT
    GCCCTGGACT TCATCCGACG TGAGTCGTCC GTCTATGACA TCTCCGAGCA CCGCCGCAGC
    TTCACGCATT CCGACTGCAA ATCCTACAAC AACCCGCCGT GCGAGGAGAA CCTCTTCAGC
    GACTACATCA GTGAGGTGGA GAGGACTTTT GGTAACCTGC AGCTGAAGGA CAGCAACGTG
    TACCAAGATC ACTATCACCA CCACCACCGG CCCCACAGCA TCGGCAGCGC CAGCTCCATC
    GACGGGCTCT ACGACTGTGA CAACCCGCCT TTCACCACCC AGCCGAGGTC CATCAGCAAG
    AAGCCCCTGG ACCTCGGCCT CCCCTCCTCC AAACACAGCC AGCTCAGTGA CCTGTACGGC
    AAATTCTCCT TCAAGAGCGA CCGCTACAGT GGCCACGATG ACTTGATCCG CTCGGACGTG
    TCTGACATCT CCACGCACAC GGTCACCTAC GGAAACATAG AGGGCAACGC CGCCAAGAGG
    CGCAAGCAGC AATACAAGGA CAGCCTGAAG AAGCGGCCGG CCTCGGCCAA GTCCCGGCGG
    GAGTTCGACG AGATCGAGCT GGCCTACCGC CGCCGGCCGC CCCGCTCCCC TGACCACAAG
    CGCTACTTCA GGGACAAGGA GGGGCTCCGG GACTTCTACC TGGACCAGTT CCGCACCAAG
    GAGAACTCCC CGCACTGGGA GCACGTGGAC CTGACGGACA TCTACAAGGA GCAGAGCGAT
    GACTTTAAGC GCGACTCGGT GAGCGGAGGA GGGCCCTGTA CCAACAGGTC TCACCTCAAA
    CACGGGGCGG GCGACAAGCA CGGCGTGGTC AGCGGGGTCC CGGCCCCTTG GGAGAAGAAC
    CTGAGCAACG TGGATTGGGA GGACCGCTCC GGGGGCAACT TTTGCCGCAG CTGCCCCTCA
    AAGCTGCACA ACTACTCGAC GGCGGTGACC GGGCAGAACT CGGGCCGGCA GGCGTGCATC
    CGGTGCGAGG CCTGCAAGAA GGCAGGGAAC CTGTACGACA TCAGCGAGGA CAACTCCCTG
    CAGGAGCTGG ACCAGCCGGC TGCCCCTGTG GCGGTGCCGT CAAACGCGCC CAGCACCAAG
    TACCCCCAGA GCCCGACCAA CTCCAAGGCC CAGAAGAAGA CCCGGAACAA GCTGCGCCGG
    CAGCACTCCT ACGACACCTT CGTGGACCTG CAGAAGGAAG AAGCCGCCTT GGCCCCGCGC
    AGCGTGAGCC TGAAAGACAA GGGCCGGTTC CTGGACGGGA GTCCCTACGC CCACATGTTT
    GAGACGCCAG CTGGCGAGAG CACCTTTGCC AACCACGAGT CCTCAGTGGC CGCCGCCGGA
    CACCGCCACC ACAACAACCC CGGCGGCGGC GGCTACATGC TCAGCAAGTC GCTCTACCCC
    GACCGGGTCA CGCAAAACCC TTTCATCCCC ACTTTTGGGG ACGACCAGTG CTTGCTCCAT
    GGCAGCAAAT CCTACTTCTT CAGGCAGCCC ACGGTGGCGG GGGCGCCGAA AGCCAGGCCA
    GACTTCCGCG CCCTTGTCAC CAACAAGCCG GTGGTCTCGG CCCTGCACGG GGCCGTGCCA
    GGCCGTTTCC AGAAAGACAT CTGTATAGGG AACCAGTCCA ACCCCTGTGT GCCTAACAAC
    AAAAACCCCA GGGCTTTCAA TGGCTCCAGC AATGGGCATG TTTATGAGAA ACTTTCTAGT
    ATTGAGTCTG ATGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26829
    Clone ID Related Accession (Same CDS sequence) XM_022410866.1 , XM_014108268.1 , XM_005636716.2 , XM_014108267.1 , NM_001008719.1
    Accession Version XM_022410866.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4458bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product glutamate receptor ionotropic, NMDA 2B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAGCCCA GAGCAGAGTG CTGTTCTCCC AAGTTCTGGT TGGTGTTGGC TGTCCTGGCT 
    GTGTCAGGCA GCAGAGCTCG TTCTCAGAAG AGCCCCCCCA GCATTGGCAT TGCTGTCATC
    CTCGTGGGCA CTTCAGACGA GGTGGCCATC AAGGATGCCC ACGAGAAAGA TGATTTCCAC
    CATCTCTCTG TGGTTCCCCG TGTGGAGCTG GTAGCCATGA ATGAGACTGA CCCAAAGAGC
    ATCATCACCC GCATCTGTGA TCTCATGTCT GACCGGAAGA TCCAGGGCGT GGTGTTTGCT
    GACGACACAG ATCAGGAGGC CATTGCCCAG ATCCTTGACT TCATTTCAGC CCAGACTCTC
    ACCCCCATCC TGGGCATCCA CGGAGGCTCC TCTATGATAA TGGCAGATAA GGATGAATCC
    TCCATGTTCT TCCAGTTTGG CCCATCAATT GAACAGCAAG CTTCTGTAAT GCTCAATATC
    ATGGAAGAGT ATGACTGGTA CATCTTTTCT ATTGTCACCA CCTACTTCCC TGGCTACCAG
    GACTTTGTAA ACAAGATTCG CAGCACCATT GAGAACAGCT TCGTGGGCTG GGAGTTAGAG
    GAAGTCCTCC TGCTGGACAT GTCCCTGGAT GATGGAGATT CTAAGATCCA GAACCAGCTC
    AAGAAACTGC AGAGCCCCAT CATTCTTCTC TATTGTACTA AGGAAGAAGC CACGTATATC
    TTTGAAGTAG CTAACTCAGT AGGGCTGACT GGCTACGGCT ACACATGGAT TGTACCTAGT
    CTGGTGGCAG GGGATACAGA CACAGTGCCC TCGGAGTTCC CCACTGGCCT CATTTCTGTG
    TCCTATGATG AATGGGACTA TGGCCTCCCT GCCAGAGTGA GGGATGGAAT TGCCATAATC
    ACCACTGCTG CTTCCGACAT GCTGTCTGAA CACAGCTTCA TCCCTGAGCC CAAGAGCAGT
    TGTTACAACA CCCATGAGAA GAGAATCTAC CAGTCCAACA TGCTAAATAG GTATCTGATC
    AATGTCACTT TTGAGGGAAG AAATCTGTCC TTCAGTGAAG ATGGCTACCA GATGCACCCG
    AAACTGGTGA TAATCCTTCT GAACAAGGAG AGAAAGTGGG AGAGGGTGGG GAAGTGGAAA
    GACAAATCCT TGCAGATGAA GTACTACGTG TGGCCCCGAA TGTGTCCTGA GACAGAAGAA
    CAGGAGGATG ACCATCTGAG CATTGTAACC CTGGAGGAGG CCCCCTTTGT CATTGTGGAG
    AGTGTGGACC CTCTGAGTGG AACCTGCATG AGGAACACAG TCCCCTGCCA AAAACGCATA
    GTCTCTGAGA ATAAAACAGA TGAAGAGCCA GGTTACATCA AAAAGTGCTG CAAGGGGTTC
    TGTATTGACA TCCTTAAGAA AATTTCTAAA TCTGTGAAGT TCACCTATGA TCTCTACCTG
    GTTACCAATG GCAAGCATGG GAAGAAAATC AATGGAACTT GGAATGGTAT GATTGGAGAG
    GTGGTTATGA AGAGGGCTTA CATGGCGGTG GGATCACTCA CCATCAATGA GGAGCGATCT
    GAGGTGGTTG ACTTCTCTGT GCCCTTCATA GAGACTGGCA TCAGTGTTAT GGTGTCACGC
    AGCAATGGGA CTGTCTCACC ATCTGCCTTC TTAGAGCCAT TCAGTGCTGA CGTATGGGTT
    ATGATGTTCG TGATGCTGCT CATCGTCTCA GCCGTGGCTG TCTTTGTCTT CGAGTACTTC
    AGCCCTGTGG GTTACAACAG GTGCCTCGCT GATGGCAGAG AGCCAGGTGG CCCGTCTTTT
    ACCATCGGCA AAGCTATTTG GTTACTGTGG GGTCTGGTGT TTAACAACTC CGTACCCGTG
    CAGAACCCAA AGGGGACCAC TTCCAAGATC ATGGTGTCGG TGTGGGCCTT CTTTGCCGTC
    ATCTTCCTGG CCAGCTACAC TGCCAACTTA GCTGCCTTCA TGATCCAAGA AGAGTATGTG
    GACCAGGTTT CTGGCCTGAG TGACAAAAAG TTCCAGAGAC CTAATGACTT CTCACCCCCT
    TTTCGCTTTG GGACTGTGCC CAATGGCAGC ACAGAGAGGA ATATTCGCAA TAACTACGCA
    GAAATGCATG CCTACATGGG AAAGTTCAAC CAGAGGGGTG TAGATGATGC ATTGCTCTCC
    CTGAAAACAG GGAAACTGGA TGCCTTCATC TATGATGCAG CAGTGCTGAA CTACATGGCG
    GGCAGAGATG AAGGCTGCAA GCTGGTGACC ATTGGCAGTG GGAAAGTCTT TGCTTCCACT
    GGTTATGGCA TTGCCATCCA AAAAGATTCT GGGTGGAAGC GCCAGGTGGA CCTTGCTATC
    CTCCAGCTAT TCGGAGATGG GGAGATGGAG GAGCTAGAAG CTCTTTGGCT CACTGGCATT
    TGCCACAATG AAAAGAATGA GGTCATGAGC AGCCAGCTGG ACATTGACAA CATGGCAGGA
    GTCTTCTACA TGTTGGGGGC AGCCATGGCT CTCAGTCTCA TCACCTTCAT CTGTGAACAC
    CTTTTCTATT GGCAGTTCCG GCACTGCTTT ATGGGTGTCT GTTCCGGCAA GCCTGGCATG
    GTCTTCTCCA TCAGCAGAGG TATCTACAGT TGCATCCATG GAGTGGCTAT CGAGGAGCGC
    CAGTCCGTGA TGAATTCCCC CACCGCGACC ATGAACAACA CACACTCCAA CATCCTGCGC
    CTGCTCCGCA CGGCCAAGAA CATGGCCAAC CTGTCTGGTG TGAACGGCTC CCCCCAGAGT
    GCCCTGGACT TCATCCGACG TGAGTCGTCC GTCTATGACA TCTCCGAGCA CCGCCGCAGC
    TTCACGCATT CCGACTGCAA ATCCTACAAC AACCCGCCGT GCGAGGAGAA CCTCTTCAGC
    GACTACATCA GTGAGGTGGA GAGGACTTTT GGTAACCTGC AGCTGAAGGA CAGCAACGTG
    TACCAAGATC ACTATCACCA CCACCACCGG CCCCACAGCA TCGGCAGCGC CAGCTCCATC
    GACGGGCTCT ACGACTGTGA CAACCCGCCT TTCACCACCC AGCCGAGGTC CATCAGCAAG
    AAGCCCCTGG ACCTCGGCCT CCCCTCCTCC AAACACAGCC AGCTCAGTGA CCTGTACGGC
    AAATTCTCCT TCAAGAGCGA CCGCTACAGT GGCCACGATG ACTTGATCCG CTCGGACGTG
    TCTGACATCT CCACGCACAC GGTCACCTAC GGAAACATAG AGGGCAACGC CGCCAAGAGG
    CGCAAGCAGC AATACAAGGA CAGCCTGAAG AAGCGGCCGG CCTCGGCCAA GTCCCGGCGG
    GAGTTCGACG AGATCGAGCT GGCCTACCGC CGCCGGCCGC CCCGCTCCCC TGACCACAAG
    CGCTACTTCA GGGACAAGGA GGGGCTCCGG GACTTCTACC TGGACCAGTT CCGCACCAAG
    GAGAACTCCC CGCACTGGGA GCACGTGGAC CTGACGGACA TCTACAAGGA GCAGAGCGAT
    GACTTTAAGC GCGACTCGGT GAGCGGAGGA GGGCCCTGTA CCAACAGGTC TCACCTCAAA
    CACGGGGCGG GCGACAAGCA CGGCGTGGTC AGCGGGGTCC CGGCCCCTTG GGAGAAGAAC
    CTGAGCAACG TGGATTGGGA GGACCGCTCC GGGGGCAACT TTTGCCGCAG CTGCCCCTCA
    AAGCTGCACA ACTACTCGAC GGCGGTGACC GGGCAGAACT CGGGCCGGCA GGCGTGCATC
    CGGTGCGAGG CCTGCAAGAA GGCAGGGAAC CTGTACGACA TCAGCGAGGA CAACTCCCTG
    CAGGAGCTGG ACCAGCCGGC TGCCCCTGTG GCGGTGCCGT CAAACGCGCC CAGCACCAAG
    TACCCCCAGA GCCCGACCAA CTCCAAGGCC CAGAAGAAGA CCCGGAACAA GCTGCGCCGG
    CAGCACTCCT ACGACACCTT CGTGGACCTG CAGAAGGAAG AAGCCGCCTT GGCCCCGCGC
    AGCGTGAGCC TGAAAGACAA GGGCCGGTTC CTGGACGGGA GTCCCTACGC CCACATGTTT
    GAGACGCCAG CTGGCGAGAG CACCTTTGCC AACCACGAGT CCTCAGTGGC CGCCGCCGGA
    CACCGCCACC ACAACAACCC CGGCGGCGGC GGCTACATGC TCAGCAAGTC GCTCTACCCC
    GACCGGGTCA CGCAAAACCC TTTCATCCCC ACTTTTGGGG ACGACCAGTG CTTGCTCCAT
    GGCAGCAAAT CCTACTTCTT CAGGCAGCCC ACGGTGGCGG GGGCGCCGAA AGCCAGGCCA
    GACTTCCGCG CCCTTGTCAC CAACAAGCCG GTGGTCTCGG CCCTGCACGG GGCCGTGCCA
    GGCCGTTTCC AGAAAGACAT CTGTATAGGG AACCAGTCCA ACCCCTGTGT GCCTAACAAC
    AAAAACCCCA GGGCTTTCAA TGGCTCCAGC AATGGGCATG TTTATGAGAA ACTTTCTAGT
    ATTGAGTCTG ATGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022266574.1
    CDS718..5175
    Translation

    Target ORF information:

    RefSeq Version XM_022410866.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris glutamate ionotropic receptor NMDA type subunit 2B (GRIN2B), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022410866.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAGCCCA GAGCAGAGTG CTGTTCTCCC AAGTTCTGGT TGGTGTTGGC TGTCCTGGCT 
    GTGTCAGGCA GCAGAGCTCG TTCTCAGAAG AGCCCCCCCA GCATTGGCAT TGCTGTCATC
    CTCGTGGGCA CTTCAGACGA GGTGGCCATC AAGGATGCCC ACGAGAAAGA TGATTTCCAC
    CATCTCTCTG TGGTTCCCCG TGTGGAGCTG GTAGCCATGA ATGAGACTGA CCCAAAGAGC
    ATCATCACCC GCATCTGTGA TCTCATGTCT GACCGGAAGA TCCAGGGCGT GGTGTTTGCT
    GACGACACAG ATCAGGAGGC CATTGCCCAG ATCCTTGACT TCATTTCAGC CCAGACTCTC
    ACCCCCATCC TGGGCATCCA CGGAGGCTCC TCTATGATAA TGGCAGATAA GGATGAATCC
    TCCATGTTCT TCCAGTTTGG CCCATCAATT GAACAGCAAG CTTCTGTAAT GCTCAATATC
    ATGGAAGAGT ATGACTGGTA CATCTTTTCT ATTGTCACCA CCTACTTCCC TGGCTACCAG
    GACTTTGTAA ACAAGATTCG CAGCACCATT GAGAACAGCT TCGTGGGCTG GGAGTTAGAG
    GAAGTCCTCC TGCTGGACAT GTCCCTGGAT GATGGAGATT CTAAGATCCA GAACCAGCTC
    AAGAAACTGC AGAGCCCCAT CATTCTTCTC TATTGTACTA AGGAAGAAGC CACGTATATC
    TTTGAAGTAG CTAACTCAGT AGGGCTGACT GGCTACGGCT ACACATGGAT TGTACCTAGT
    CTGGTGGCAG GGGATACAGA CACAGTGCCC TCGGAGTTCC CCACTGGCCT CATTTCTGTG
    TCCTATGATG AATGGGACTA TGGCCTCCCT GCCAGAGTGA GGGATGGAAT TGCCATAATC
    ACCACTGCTG CTTCCGACAT GCTGTCTGAA CACAGCTTCA TCCCTGAGCC CAAGAGCAGT
    TGTTACAACA CCCATGAGAA GAGAATCTAC CAGTCCAACA TGCTAAATAG GTATCTGATC
    AATGTCACTT TTGAGGGAAG AAATCTGTCC TTCAGTGAAG ATGGCTACCA GATGCACCCG
    AAACTGGTGA TAATCCTTCT GAACAAGGAG AGAAAGTGGG AGAGGGTGGG GAAGTGGAAA
    GACAAATCCT TGCAGATGAA GTACTACGTG TGGCCCCGAA TGTGTCCTGA GACAGAAGAA
    CAGGAGGATG ACCATCTGAG CATTGTAACC CTGGAGGAGG CCCCCTTTGT CATTGTGGAG
    AGTGTGGACC CTCTGAGTGG AACCTGCATG AGGAACACAG TCCCCTGCCA AAAACGCATA
    GTCTCTGAGA ATAAAACAGA TGAAGAGCCA GGTTACATCA AAAAGTGCTG CAAGGGGTTC
    TGTATTGACA TCCTTAAGAA AATTTCTAAA TCTGTGAAGT TCACCTATGA TCTCTACCTG
    GTTACCAATG GCAAGCATGG GAAGAAAATC AATGGAACTT GGAATGGTAT GATTGGAGAG
    GTGGTTATGA AGAGGGCTTA CATGGCGGTG GGATCACTCA CCATCAATGA GGAGCGATCT
    GAGGTGGTTG ACTTCTCTGT GCCCTTCATA GAGACTGGCA TCAGTGTTAT GGTGTCACGC
    AGCAATGGGA CTGTCTCACC ATCTGCCTTC TTAGAGCCAT TCAGTGCTGA CGTATGGGTT
    ATGATGTTCG TGATGCTGCT CATCGTCTCA GCCGTGGCTG TCTTTGTCTT CGAGTACTTC
    AGCCCTGTGG GTTACAACAG GTGCCTCGCT GATGGCAGAG AGCCAGGTGG CCCGTCTTTT
    ACCATCGGCA AAGCTATTTG GTTACTGTGG GGTCTGGTGT TTAACAACTC CGTACCCGTG
    CAGAACCCAA AGGGGACCAC TTCCAAGATC ATGGTGTCGG TGTGGGCCTT CTTTGCCGTC
    ATCTTCCTGG CCAGCTACAC TGCCAACTTA GCTGCCTTCA TGATCCAAGA AGAGTATGTG
    GACCAGGTTT CTGGCCTGAG TGACAAAAAG TTCCAGAGAC CTAATGACTT CTCACCCCCT
    TTTCGCTTTG GGACTGTGCC CAATGGCAGC ACAGAGAGGA ATATTCGCAA TAACTACGCA
    GAAATGCATG CCTACATGGG AAAGTTCAAC CAGAGGGGTG TAGATGATGC ATTGCTCTCC
    CTGAAAACAG GGAAACTGGA TGCCTTCATC TATGATGCAG CAGTGCTGAA CTACATGGCG
    GGCAGAGATG AAGGCTGCAA GCTGGTGACC ATTGGCAGTG GGAAAGTCTT TGCTTCCACT
    GGTTATGGCA TTGCCATCCA AAAAGATTCT GGGTGGAAGC GCCAGGTGGA CCTTGCTATC
    CTCCAGCTAT TCGGAGATGG GGAGATGGAG GAGCTAGAAG CTCTTTGGCT CACTGGCATT
    TGCCACAATG AAAAGAATGA GGTCATGAGC AGCCAGCTGG ACATTGACAA CATGGCAGGA
    GTCTTCTACA TGTTGGGGGC AGCCATGGCT CTCAGTCTCA TCACCTTCAT CTGTGAACAC
    CTTTTCTATT GGCAGTTCCG GCACTGCTTT ATGGGTGTCT GTTCCGGCAA GCCTGGCATG
    GTCTTCTCCA TCAGCAGAGG TATCTACAGT TGCATCCATG GAGTGGCTAT CGAGGAGCGC
    CAGTCCGTGA TGAATTCCCC CACCGCGACC ATGAACAACA CACACTCCAA CATCCTGCGC
    CTGCTCCGCA CGGCCAAGAA CATGGCCAAC CTGTCTGGTG TGAACGGCTC CCCCCAGAGT
    GCCCTGGACT TCATCCGACG TGAGTCGTCC GTCTATGACA TCTCCGAGCA CCGCCGCAGC
    TTCACGCATT CCGACTGCAA ATCCTACAAC AACCCGCCGT GCGAGGAGAA CCTCTTCAGC
    GACTACATCA GTGAGGTGGA GAGGACTTTT GGTAACCTGC AGCTGAAGGA CAGCAACGTG
    TACCAAGATC ACTATCACCA CCACCACCGG CCCCACAGCA TCGGCAGCGC CAGCTCCATC
    GACGGGCTCT ACGACTGTGA CAACCCGCCT TTCACCACCC AGCCGAGGTC CATCAGCAAG
    AAGCCCCTGG ACCTCGGCCT CCCCTCCTCC AAACACAGCC AGCTCAGTGA CCTGTACGGC
    AAATTCTCCT TCAAGAGCGA CCGCTACAGT GGCCACGATG ACTTGATCCG CTCGGACGTG
    TCTGACATCT CCACGCACAC GGTCACCTAC GGAAACATAG AGGGCAACGC CGCCAAGAGG
    CGCAAGCAGC AATACAAGGA CAGCCTGAAG AAGCGGCCGG CCTCGGCCAA GTCCCGGCGG
    GAGTTCGACG AGATCGAGCT GGCCTACCGC CGCCGGCCGC CCCGCTCCCC TGACCACAAG
    CGCTACTTCA GGGACAAGGA GGGGCTCCGG GACTTCTACC TGGACCAGTT CCGCACCAAG
    GAGAACTCCC CGCACTGGGA GCACGTGGAC CTGACGGACA TCTACAAGGA GCAGAGCGAT
    GACTTTAAGC GCGACTCGGT GAGCGGAGGA GGGCCCTGTA CCAACAGGTC TCACCTCAAA
    CACGGGGCGG GCGACAAGCA CGGCGTGGTC AGCGGGGTCC CGGCCCCTTG GGAGAAGAAC
    CTGAGCAACG TGGATTGGGA GGACCGCTCC GGGGGCAACT TTTGCCGCAG CTGCCCCTCA
    AAGCTGCACA ACTACTCGAC GGCGGTGACC GGGCAGAACT CGGGCCGGCA GGCGTGCATC
    CGGTGCGAGG CCTGCAAGAA GGCAGGGAAC CTGTACGACA TCAGCGAGGA CAACTCCCTG
    CAGGAGCTGG ACCAGCCGGC TGCCCCTGTG GCGGTGCCGT CAAACGCGCC CAGCACCAAG
    TACCCCCAGA GCCCGACCAA CTCCAAGGCC CAGAAGAAGA CCCGGAACAA GCTGCGCCGG
    CAGCACTCCT ACGACACCTT CGTGGACCTG CAGAAGGAAG AAGCCGCCTT GGCCCCGCGC
    AGCGTGAGCC TGAAAGACAA GGGCCGGTTC CTGGACGGGA GTCCCTACGC CCACATGTTT
    GAGACGCCAG CTGGCGAGAG CACCTTTGCC AACCACGAGT CCTCAGTGGC CGCCGCCGGA
    CACCGCCACC ACAACAACCC CGGCGGCGGC GGCTACATGC TCAGCAAGTC GCTCTACCCC
    GACCGGGTCA CGCAAAACCC TTTCATCCCC ACTTTTGGGG ACGACCAGTG CTTGCTCCAT
    GGCAGCAAAT CCTACTTCTT CAGGCAGCCC ACGGTGGCGG GGGCGCCGAA AGCCAGGCCA
    GACTTCCGCG CCCTTGTCAC CAACAAGCCG GTGGTCTCGG CCCTGCACGG GGCCGTGCCA
    GGCCGTTTCC AGAAAGACAT CTGTATAGGG AACCAGTCCA ACCCCTGTGT GCCTAACAAC
    AAAAACCCCA GGGCTTTCAA TGGCTCCAGC AATGGGCATG TTTATGAGAA ACTTTCTAGT
    ATTGAGTCTG ATGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26829
    Clone ID Related Accession (Same CDS sequence) XM_022410866.1 , XM_014108268.1 , XM_005636716.2 , XM_014108267.1 , NM_001008719.1
    Accession Version XM_014108268.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4458bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product glutamate receptor ionotropic, NMDA 2B isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAGCCCA GAGCAGAGTG CTGTTCTCCC AAGTTCTGGT TGGTGTTGGC TGTCCTGGCT 
    GTGTCAGGCA GCAGAGCTCG TTCTCAGAAG AGCCCCCCCA GCATTGGCAT TGCTGTCATC
    CTCGTGGGCA CTTCAGACGA GGTGGCCATC AAGGATGCCC ACGAGAAAGA TGATTTCCAC
    CATCTCTCTG TGGTTCCCCG TGTGGAGCTG GTAGCCATGA ATGAGACTGA CCCAAAGAGC
    ATCATCACCC GCATCTGTGA TCTCATGTCT GACCGGAAGA TCCAGGGCGT GGTGTTTGCT
    GACGACACAG ATCAGGAGGC CATTGCCCAG ATCCTTGACT TCATTTCAGC CCAGACTCTC
    ACCCCCATCC TGGGCATCCA CGGAGGCTCC TCTATGATAA TGGCAGATAA GGATGAATCC
    TCCATGTTCT TCCAGTTTGG CCCATCAATT GAACAGCAAG CTTCTGTAAT GCTCAATATC
    ATGGAAGAGT ATGACTGGTA CATCTTTTCT ATTGTCACCA CCTACTTCCC TGGCTACCAG
    GACTTTGTAA ACAAGATTCG CAGCACCATT GAGAACAGCT TCGTGGGCTG GGAGTTAGAG
    GAAGTCCTCC TGCTGGACAT GTCCCTGGAT GATGGAGATT CTAAGATCCA GAACCAGCTC
    AAGAAACTGC AGAGCCCCAT CATTCTTCTC TATTGTACTA AGGAAGAAGC CACGTATATC
    TTTGAAGTAG CTAACTCAGT AGGGCTGACT GGCTACGGCT ACACATGGAT TGTACCTAGT
    CTGGTGGCAG GGGATACAGA CACAGTGCCC TCGGAGTTCC CCACTGGCCT CATTTCTGTG
    TCCTATGATG AATGGGACTA TGGCCTCCCT GCCAGAGTGA GGGATGGAAT TGCCATAATC
    ACCACTGCTG CTTCCGACAT GCTGTCTGAA CACAGCTTCA TCCCTGAGCC CAAGAGCAGT
    TGTTACAACA CCCATGAGAA GAGAATCTAC CAGTCCAACA TGCTAAATAG GTATCTGATC
    AATGTCACTT TTGAGGGAAG AAATCTGTCC TTCAGTGAAG ATGGCTACCA GATGCACCCG
    AAACTGGTGA TAATCCTTCT GAACAAGGAG AGAAAGTGGG AGAGGGTGGG GAAGTGGAAA
    GACAAATCCT TGCAGATGAA GTACTACGTG TGGCCCCGAA TGTGTCCTGA GACAGAAGAA
    CAGGAGGATG ACCATCTGAG CATTGTAACC CTGGAGGAGG CCCCCTTTGT CATTGTGGAG
    AGTGTGGACC CTCTGAGTGG AACCTGCATG AGGAACACAG TCCCCTGCCA AAAACGCATA
    GTCTCTGAGA ATAAAACAGA TGAAGAGCCA GGTTACATCA AAAAGTGCTG CAAGGGGTTC
    TGTATTGACA TCCTTAAGAA AATTTCTAAA TCTGTGAAGT TCACCTATGA TCTCTACCTG
    GTTACCAATG GCAAGCATGG GAAGAAAATC AATGGAACTT GGAATGGTAT GATTGGAGAG
    GTGGTTATGA AGAGGGCTTA CATGGCGGTG GGATCACTCA CCATCAATGA GGAGCGATCT
    GAGGTGGTTG ACTTCTCTGT GCCCTTCATA GAGACTGGCA TCAGTGTTAT GGTGTCACGC
    AGCAATGGGA CTGTCTCACC ATCTGCCTTC TTAGAGCCAT TCAGTGCTGA CGTATGGGTT
    ATGATGTTCG TGATGCTGCT CATCGTCTCA GCCGTGGCTG TCTTTGTCTT CGAGTACTTC
    AGCCCTGTGG GTTACAACAG GTGCCTCGCT GATGGCAGAG AGCCAGGTGG CCCGTCTTTT
    ACCATCGGCA AAGCTATTTG GTTACTGTGG GGTCTGGTGT TTAACAACTC CGTACCCGTG
    CAGAACCCAA AGGGGACCAC TTCCAAGATC ATGGTGTCGG TGTGGGCCTT CTTTGCCGTC
    ATCTTCCTGG CCAGCTACAC TGCCAACTTA GCTGCCTTCA TGATCCAAGA AGAGTATGTG
    GACCAGGTTT CTGGCCTGAG TGACAAAAAG TTCCAGAGAC CTAATGACTT CTCACCCCCT
    TTTCGCTTTG GGACTGTGCC CAATGGCAGC ACAGAGAGGA ATATTCGCAA TAACTACGCA
    GAAATGCATG CCTACATGGG AAAGTTCAAC CAGAGGGGTG TAGATGATGC ATTGCTCTCC
    CTGAAAACAG GGAAACTGGA TGCCTTCATC TATGATGCAG CAGTGCTGAA CTACATGGCG
    GGCAGAGATG AAGGCTGCAA GCTGGTGACC ATTGGCAGTG GGAAAGTCTT TGCTTCCACT
    GGTTATGGCA TTGCCATCCA AAAAGATTCT GGGTGGAAGC GCCAGGTGGA CCTTGCTATC
    CTCCAGCTAT TCGGAGATGG GGAGATGGAG GAGCTAGAAG CTCTTTGGCT CACTGGCATT
    TGCCACAATG AAAAGAATGA GGTCATGAGC AGCCAGCTGG ACATTGACAA CATGGCAGGA
    GTCTTCTACA TGTTGGGGGC AGCCATGGCT CTCAGTCTCA TCACCTTCAT CTGTGAACAC
    CTTTTCTATT GGCAGTTCCG GCACTGCTTT ATGGGTGTCT GTTCCGGCAA GCCTGGCATG
    GTCTTCTCCA TCAGCAGAGG TATCTACAGT TGCATCCATG GAGTGGCTAT CGAGGAGCGC
    CAGTCCGTGA TGAATTCCCC CACCGCGACC ATGAACAACA CACACTCCAA CATCCTGCGC
    CTGCTCCGCA CGGCCAAGAA CATGGCCAAC CTGTCTGGTG TGAACGGCTC CCCCCAGAGT
    GCCCTGGACT TCATCCGACG TGAGTCGTCC GTCTATGACA TCTCCGAGCA CCGCCGCAGC
    TTCACGCATT CCGACTGCAA ATCCTACAAC AACCCGCCGT GCGAGGAGAA CCTCTTCAGC
    GACTACATCA GTGAGGTGGA GAGGACTTTT GGTAACCTGC AGCTGAAGGA CAGCAACGTG
    TACCAAGATC ACTATCACCA CCACCACCGG CCCCACAGCA TCGGCAGCGC CAGCTCCATC
    GACGGGCTCT ACGACTGTGA CAACCCGCCT TTCACCACCC AGCCGAGGTC CATCAGCAAG
    AAGCCCCTGG ACCTCGGCCT CCCCTCCTCC AAACACAGCC AGCTCAGTGA CCTGTACGGC
    AAATTCTCCT TCAAGAGCGA CCGCTACAGT GGCCACGATG ACTTGATCCG CTCGGACGTG
    TCTGACATCT CCACGCACAC GGTCACCTAC GGAAACATAG AGGGCAACGC CGCCAAGAGG
    CGCAAGCAGC AATACAAGGA CAGCCTGAAG AAGCGGCCGG CCTCGGCCAA GTCCCGGCGG
    GAGTTCGACG AGATCGAGCT GGCCTACCGC CGCCGGCCGC CCCGCTCCCC TGACCACAAG
    CGCTACTTCA GGGACAAGGA GGGGCTCCGG GACTTCTACC TGGACCAGTT CCGCACCAAG
    GAGAACTCCC CGCACTGGGA GCACGTGGAC CTGACGGACA TCTACAAGGA GCAGAGCGAT
    GACTTTAAGC GCGACTCGGT GAGCGGAGGA GGGCCCTGTA CCAACAGGTC TCACCTCAAA
    CACGGGGCGG GCGACAAGCA CGGCGTGGTC AGCGGGGTCC CGGCCCCTTG GGAGAAGAAC
    CTGAGCAACG TGGATTGGGA GGACCGCTCC GGGGGCAACT TTTGCCGCAG CTGCCCCTCA
    AAGCTGCACA ACTACTCGAC GGCGGTGACC GGGCAGAACT CGGGCCGGCA GGCGTGCATC
    CGGTGCGAGG CCTGCAAGAA GGCAGGGAAC CTGTACGACA TCAGCGAGGA CAACTCCCTG
    CAGGAGCTGG ACCAGCCGGC TGCCCCTGTG GCGGTGCCGT CAAACGCGCC CAGCACCAAG
    TACCCCCAGA GCCCGACCAA CTCCAAGGCC CAGAAGAAGA CCCGGAACAA GCTGCGCCGG
    CAGCACTCCT ACGACACCTT CGTGGACCTG CAGAAGGAAG AAGCCGCCTT GGCCCCGCGC
    AGCGTGAGCC TGAAAGACAA GGGCCGGTTC CTGGACGGGA GTCCCTACGC CCACATGTTT
    GAGACGCCAG CTGGCGAGAG CACCTTTGCC AACCACGAGT CCTCAGTGGC CGCCGCCGGA
    CACCGCCACC ACAACAACCC CGGCGGCGGC GGCTACATGC TCAGCAAGTC GCTCTACCCC
    GACCGGGTCA CGCAAAACCC TTTCATCCCC ACTTTTGGGG ACGACCAGTG CTTGCTCCAT
    GGCAGCAAAT CCTACTTCTT CAGGCAGCCC ACGGTGGCGG GGGCGCCGAA AGCCAGGCCA
    GACTTCCGCG CCCTTGTCAC CAACAAGCCG GTGGTCTCGG CCCTGCACGG GGCCGTGCCA
    GGCCGTTTCC AGAAAGACAT CTGTATAGGG AACCAGTCCA ACCCCTGTGT GCCTAACAAC
    AAAAACCCCA GGGCTTTCAA TGGCTCCAGC AATGGGCATG TTTATGAGAA ACTTTCTAGT
    ATTGAGTCTG ATGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963743.1
    CDS474..4931
    Translation

    Target ORF information:

    RefSeq Version XM_014108268.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris glutamate receptor, ionotropic, N-methyl D-aspartate 2B (GRIN2B), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014108268.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAGCCCA GAGCAGAGTG CTGTTCTCCC AAGTTCTGGT TGGTGTTGGC TGTCCTGGCT 
    GTGTCAGGCA GCAGAGCTCG TTCTCAGAAG AGCCCCCCCA GCATTGGCAT TGCTGTCATC
    CTCGTGGGCA CTTCAGACGA GGTGGCCATC AAGGATGCCC ACGAGAAAGA TGATTTCCAC
    CATCTCTCTG TGGTTCCCCG TGTGGAGCTG GTAGCCATGA ATGAGACTGA CCCAAAGAGC
    ATCATCACCC GCATCTGTGA TCTCATGTCT GACCGGAAGA TCCAGGGCGT GGTGTTTGCT
    GACGACACAG ATCAGGAGGC CATTGCCCAG ATCCTTGACT TCATTTCAGC CCAGACTCTC
    ACCCCCATCC TGGGCATCCA CGGAGGCTCC TCTATGATAA TGGCAGATAA GGATGAATCC
    TCCATGTTCT TCCAGTTTGG CCCATCAATT GAACAGCAAG CTTCTGTAAT GCTCAATATC
    ATGGAAGAGT ATGACTGGTA CATCTTTTCT ATTGTCACCA CCTACTTCCC TGGCTACCAG
    GACTTTGTAA ACAAGATTCG CAGCACCATT GAGAACAGCT TCGTGGGCTG GGAGTTAGAG
    GAAGTCCTCC TGCTGGACAT GTCCCTGGAT GATGGAGATT CTAAGATCCA GAACCAGCTC
    AAGAAACTGC AGAGCCCCAT CATTCTTCTC TATTGTACTA AGGAAGAAGC CACGTATATC
    TTTGAAGTAG CTAACTCAGT AGGGCTGACT GGCTACGGCT ACACATGGAT TGTACCTAGT
    CTGGTGGCAG GGGATACAGA CACAGTGCCC TCGGAGTTCC CCACTGGCCT CATTTCTGTG
    TCCTATGATG AATGGGACTA TGGCCTCCCT GCCAGAGTGA GGGATGGAAT TGCCATAATC
    ACCACTGCTG CTTCCGACAT GCTGTCTGAA CACAGCTTCA TCCCTGAGCC CAAGAGCAGT
    TGTTACAACA CCCATGAGAA GAGAATCTAC CAGTCCAACA TGCTAAATAG GTATCTGATC
    AATGTCACTT TTGAGGGAAG AAATCTGTCC TTCAGTGAAG ATGGCTACCA GATGCACCCG
    AAACTGGTGA TAATCCTTCT GAACAAGGAG AGAAAGTGGG AGAGGGTGGG GAAGTGGAAA
    GACAAATCCT TGCAGATGAA GTACTACGTG TGGCCCCGAA TGTGTCCTGA GACAGAAGAA
    CAGGAGGATG ACCATCTGAG CATTGTAACC CTGGAGGAGG CCCCCTTTGT CATTGTGGAG
    AGTGTGGACC CTCTGAGTGG AACCTGCATG AGGAACACAG TCCCCTGCCA AAAACGCATA
    GTCTCTGAGA ATAAAACAGA TGAAGAGCCA GGTTACATCA AAAAGTGCTG CAAGGGGTTC
    TGTATTGACA TCCTTAAGAA AATTTCTAAA TCTGTGAAGT TCACCTATGA TCTCTACCTG
    GTTACCAATG GCAAGCATGG GAAGAAAATC AATGGAACTT GGAATGGTAT GATTGGAGAG
    GTGGTTATGA AGAGGGCTTA CATGGCGGTG GGATCACTCA CCATCAATGA GGAGCGATCT
    GAGGTGGTTG ACTTCTCTGT GCCCTTCATA GAGACTGGCA TCAGTGTTAT GGTGTCACGC
    AGCAATGGGA CTGTCTCACC ATCTGCCTTC TTAGAGCCAT TCAGTGCTGA CGTATGGGTT
    ATGATGTTCG TGATGCTGCT CATCGTCTCA GCCGTGGCTG TCTTTGTCTT CGAGTACTTC
    AGCCCTGTGG GTTACAACAG GTGCCTCGCT GATGGCAGAG AGCCAGGTGG CCCGTCTTTT
    ACCATCGGCA AAGCTATTTG GTTACTGTGG GGTCTGGTGT TTAACAACTC CGTACCCGTG
    CAGAACCCAA AGGGGACCAC TTCCAAGATC ATGGTGTCGG TGTGGGCCTT CTTTGCCGTC
    ATCTTCCTGG CCAGCTACAC TGCCAACTTA GCTGCCTTCA TGATCCAAGA AGAGTATGTG
    GACCAGGTTT CTGGCCTGAG TGACAAAAAG TTCCAGAGAC CTAATGACTT CTCACCCCCT
    TTTCGCTTTG GGACTGTGCC CAATGGCAGC ACAGAGAGGA ATATTCGCAA TAACTACGCA
    GAAATGCATG CCTACATGGG AAAGTTCAAC CAGAGGGGTG TAGATGATGC ATTGCTCTCC
    CTGAAAACAG GGAAACTGGA TGCCTTCATC TATGATGCAG CAGTGCTGAA CTACATGGCG
    GGCAGAGATG AAGGCTGCAA GCTGGTGACC ATTGGCAGTG GGAAAGTCTT TGCTTCCACT
    GGTTATGGCA TTGCCATCCA AAAAGATTCT GGGTGGAAGC GCCAGGTGGA CCTTGCTATC
    CTCCAGCTAT TCGGAGATGG GGAGATGGAG GAGCTAGAAG CTCTTTGGCT CACTGGCATT
    TGCCACAATG AAAAGAATGA GGTCATGAGC AGCCAGCTGG ACATTGACAA CATGGCAGGA
    GTCTTCTACA TGTTGGGGGC AGCCATGGCT CTCAGTCTCA TCACCTTCAT CTGTGAACAC
    CTTTTCTATT GGCAGTTCCG GCACTGCTTT ATGGGTGTCT GTTCCGGCAA GCCTGGCATG
    GTCTTCTCCA TCAGCAGAGG TATCTACAGT TGCATCCATG GAGTGGCTAT CGAGGAGCGC
    CAGTCCGTGA TGAATTCCCC CACCGCGACC ATGAACAACA CACACTCCAA CATCCTGCGC
    CTGCTCCGCA CGGCCAAGAA CATGGCCAAC CTGTCTGGTG TGAACGGCTC CCCCCAGAGT
    GCCCTGGACT TCATCCGACG TGAGTCGTCC GTCTATGACA TCTCCGAGCA CCGCCGCAGC
    TTCACGCATT CCGACTGCAA ATCCTACAAC AACCCGCCGT GCGAGGAGAA CCTCTTCAGC
    GACTACATCA GTGAGGTGGA GAGGACTTTT GGTAACCTGC AGCTGAAGGA CAGCAACGTG
    TACCAAGATC ACTATCACCA CCACCACCGG CCCCACAGCA TCGGCAGCGC CAGCTCCATC
    GACGGGCTCT ACGACTGTGA CAACCCGCCT TTCACCACCC AGCCGAGGTC CATCAGCAAG
    AAGCCCCTGG ACCTCGGCCT CCCCTCCTCC AAACACAGCC AGCTCAGTGA CCTGTACGGC
    AAATTCTCCT TCAAGAGCGA CCGCTACAGT GGCCACGATG ACTTGATCCG CTCGGACGTG
    TCTGACATCT CCACGCACAC GGTCACCTAC GGAAACATAG AGGGCAACGC CGCCAAGAGG
    CGCAAGCAGC AATACAAGGA CAGCCTGAAG AAGCGGCCGG CCTCGGCCAA GTCCCGGCGG
    GAGTTCGACG AGATCGAGCT GGCCTACCGC CGCCGGCCGC CCCGCTCCCC TGACCACAAG
    CGCTACTTCA GGGACAAGGA GGGGCTCCGG GACTTCTACC TGGACCAGTT CCGCACCAAG
    GAGAACTCCC CGCACTGGGA GCACGTGGAC CTGACGGACA TCTACAAGGA GCAGAGCGAT
    GACTTTAAGC GCGACTCGGT GAGCGGAGGA GGGCCCTGTA CCAACAGGTC TCACCTCAAA
    CACGGGGCGG GCGACAAGCA CGGCGTGGTC AGCGGGGTCC CGGCCCCTTG GGAGAAGAAC
    CTGAGCAACG TGGATTGGGA GGACCGCTCC GGGGGCAACT TTTGCCGCAG CTGCCCCTCA
    AAGCTGCACA ACTACTCGAC GGCGGTGACC GGGCAGAACT CGGGCCGGCA GGCGTGCATC
    CGGTGCGAGG CCTGCAAGAA GGCAGGGAAC CTGTACGACA TCAGCGAGGA CAACTCCCTG
    CAGGAGCTGG ACCAGCCGGC TGCCCCTGTG GCGGTGCCGT CAAACGCGCC CAGCACCAAG
    TACCCCCAGA GCCCGACCAA CTCCAAGGCC CAGAAGAAGA CCCGGAACAA GCTGCGCCGG
    CAGCACTCCT ACGACACCTT CGTGGACCTG CAGAAGGAAG AAGCCGCCTT GGCCCCGCGC
    AGCGTGAGCC TGAAAGACAA GGGCCGGTTC CTGGACGGGA GTCCCTACGC CCACATGTTT
    GAGACGCCAG CTGGCGAGAG CACCTTTGCC AACCACGAGT CCTCAGTGGC CGCCGCCGGA
    CACCGCCACC ACAACAACCC CGGCGGCGGC GGCTACATGC TCAGCAAGTC GCTCTACCCC
    GACCGGGTCA CGCAAAACCC TTTCATCCCC ACTTTTGGGG ACGACCAGTG CTTGCTCCAT
    GGCAGCAAAT CCTACTTCTT CAGGCAGCCC ACGGTGGCGG GGGCGCCGAA AGCCAGGCCA
    GACTTCCGCG CCCTTGTCAC CAACAAGCCG GTGGTCTCGG CCCTGCACGG GGCCGTGCCA
    GGCCGTTTCC AGAAAGACAT CTGTATAGGG AACCAGTCCA ACCCCTGTGT GCCTAACAAC
    AAAAACCCCA GGGCTTTCAA TGGCTCCAGC AATGGGCATG TTTATGAGAA ACTTTCTAGT
    ATTGAGTCTG ATGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26829
    Clone ID Related Accession (Same CDS sequence) XM_022410866.1 , XM_014108268.1 , XM_005636716.2 , XM_014108267.1 , NM_001008719.1
    Accession Version XM_005636716.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4458bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product glutamate receptor ionotropic, NMDA 2B isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005636716.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAGCCCA GAGCAGAGTG CTGTTCTCCC AAGTTCTGGT TGGTGTTGGC TGTCCTGGCT 
    GTGTCAGGCA GCAGAGCTCG TTCTCAGAAG AGCCCCCCCA GCATTGGCAT TGCTGTCATC
    CTCGTGGGCA CTTCAGACGA GGTGGCCATC AAGGATGCCC ACGAGAAAGA TGATTTCCAC
    CATCTCTCTG TGGTTCCCCG TGTGGAGCTG GTAGCCATGA ATGAGACTGA CCCAAAGAGC
    ATCATCACCC GCATCTGTGA TCTCATGTCT GACCGGAAGA TCCAGGGCGT GGTGTTTGCT
    GACGACACAG ATCAGGAGGC CATTGCCCAG ATCCTTGACT TCATTTCAGC CCAGACTCTC
    ACCCCCATCC TGGGCATCCA CGGAGGCTCC TCTATGATAA TGGCAGATAA GGATGAATCC
    TCCATGTTCT TCCAGTTTGG CCCATCAATT GAACAGCAAG CTTCTGTAAT GCTCAATATC
    ATGGAAGAGT ATGACTGGTA CATCTTTTCT ATTGTCACCA CCTACTTCCC TGGCTACCAG
    GACTTTGTAA ACAAGATTCG CAGCACCATT GAGAACAGCT TCGTGGGCTG GGAGTTAGAG
    GAAGTCCTCC TGCTGGACAT GTCCCTGGAT GATGGAGATT CTAAGATCCA GAACCAGCTC
    AAGAAACTGC AGAGCCCCAT CATTCTTCTC TATTGTACTA AGGAAGAAGC CACGTATATC
    TTTGAAGTAG CTAACTCAGT AGGGCTGACT GGCTACGGCT ACACATGGAT TGTACCTAGT
    CTGGTGGCAG GGGATACAGA CACAGTGCCC TCGGAGTTCC CCACTGGCCT CATTTCTGTG
    TCCTATGATG AATGGGACTA TGGCCTCCCT GCCAGAGTGA GGGATGGAAT TGCCATAATC
    ACCACTGCTG CTTCCGACAT GCTGTCTGAA CACAGCTTCA TCCCTGAGCC CAAGAGCAGT
    TGTTACAACA CCCATGAGAA GAGAATCTAC CAGTCCAACA TGCTAAATAG GTATCTGATC
    AATGTCACTT TTGAGGGAAG AAATCTGTCC TTCAGTGAAG ATGGCTACCA GATGCACCCG
    AAACTGGTGA TAATCCTTCT GAACAAGGAG AGAAAGTGGG AGAGGGTGGG GAAGTGGAAA
    GACAAATCCT TGCAGATGAA GTACTACGTG TGGCCCCGAA TGTGTCCTGA GACAGAAGAA
    CAGGAGGATG ACCATCTGAG CATTGTAACC CTGGAGGAGG CCCCCTTTGT CATTGTGGAG
    AGTGTGGACC CTCTGAGTGG AACCTGCATG AGGAACACAG TCCCCTGCCA AAAACGCATA
    GTCTCTGAGA ATAAAACAGA TGAAGAGCCA GGTTACATCA AAAAGTGCTG CAAGGGGTTC
    TGTATTGACA TCCTTAAGAA AATTTCTAAA TCTGTGAAGT TCACCTATGA TCTCTACCTG
    GTTACCAATG GCAAGCATGG GAAGAAAATC AATGGAACTT GGAATGGTAT GATTGGAGAG
    GTGGTTATGA AGAGGGCTTA CATGGCGGTG GGATCACTCA CCATCAATGA GGAGCGATCT
    GAGGTGGTTG ACTTCTCTGT GCCCTTCATA GAGACTGGCA TCAGTGTTAT GGTGTCACGC
    AGCAATGGGA CTGTCTCACC ATCTGCCTTC TTAGAGCCAT TCAGTGCTGA CGTATGGGTT
    ATGATGTTCG TGATGCTGCT CATCGTCTCA GCCGTGGCTG TCTTTGTCTT CGAGTACTTC
    AGCCCTGTGG GTTACAACAG GTGCCTCGCT GATGGCAGAG AGCCAGGTGG CCCGTCTTTT
    ACCATCGGCA AAGCTATTTG GTTACTGTGG GGTCTGGTGT TTAACAACTC CGTACCCGTG
    CAGAACCCAA AGGGGACCAC TTCCAAGATC ATGGTGTCGG TGTGGGCCTT CTTTGCCGTC
    ATCTTCCTGG CCAGCTACAC TGCCAACTTA GCTGCCTTCA TGATCCAAGA AGAGTATGTG
    GACCAGGTTT CTGGCCTGAG TGACAAAAAG TTCCAGAGAC CTAATGACTT CTCACCCCCT
    TTTCGCTTTG GGACTGTGCC CAATGGCAGC ACAGAGAGGA ATATTCGCAA TAACTACGCA
    GAAATGCATG CCTACATGGG AAAGTTCAAC CAGAGGGGTG TAGATGATGC ATTGCTCTCC
    CTGAAAACAG GGAAACTGGA TGCCTTCATC TATGATGCAG CAGTGCTGAA CTACATGGCG
    GGCAGAGATG AAGGCTGCAA GCTGGTGACC ATTGGCAGTG GGAAAGTCTT TGCTTCCACT
    GGTTATGGCA TTGCCATCCA AAAAGATTCT GGGTGGAAGC GCCAGGTGGA CCTTGCTATC
    CTCCAGCTAT TCGGAGATGG GGAGATGGAG GAGCTAGAAG CTCTTTGGCT CACTGGCATT
    TGCCACAATG AAAAGAATGA GGTCATGAGC AGCCAGCTGG ACATTGACAA CATGGCAGGA
    GTCTTCTACA TGTTGGGGGC AGCCATGGCT CTCAGTCTCA TCACCTTCAT CTGTGAACAC
    CTTTTCTATT GGCAGTTCCG GCACTGCTTT ATGGGTGTCT GTTCCGGCAA GCCTGGCATG
    GTCTTCTCCA TCAGCAGAGG TATCTACAGT TGCATCCATG GAGTGGCTAT CGAGGAGCGC
    CAGTCCGTGA TGAATTCCCC CACCGCGACC ATGAACAACA CACACTCCAA CATCCTGCGC
    CTGCTCCGCA CGGCCAAGAA CATGGCCAAC CTGTCTGGTG TGAACGGCTC CCCCCAGAGT
    GCCCTGGACT TCATCCGACG TGAGTCGTCC GTCTATGACA TCTCCGAGCA CCGCCGCAGC
    TTCACGCATT CCGACTGCAA ATCCTACAAC AACCCGCCGT GCGAGGAGAA CCTCTTCAGC
    GACTACATCA GTGAGGTGGA GAGGACTTTT GGTAACCTGC AGCTGAAGGA CAGCAACGTG
    TACCAAGATC ACTATCACCA CCACCACCGG CCCCACAGCA TCGGCAGCGC CAGCTCCATC
    GACGGGCTCT ACGACTGTGA CAACCCGCCT TTCACCACCC AGCCGAGGTC CATCAGCAAG
    AAGCCCCTGG ACCTCGGCCT CCCCTCCTCC AAACACAGCC AGCTCAGTGA CCTGTACGGC
    AAATTCTCCT TCAAGAGCGA CCGCTACAGT GGCCACGATG ACTTGATCCG CTCGGACGTG
    TCTGACATCT CCACGCACAC GGTCACCTAC GGAAACATAG AGGGCAACGC CGCCAAGAGG
    CGCAAGCAGC AATACAAGGA CAGCCTGAAG AAGCGGCCGG CCTCGGCCAA GTCCCGGCGG
    GAGTTCGACG AGATCGAGCT GGCCTACCGC CGCCGGCCGC CCCGCTCCCC TGACCACAAG
    CGCTACTTCA GGGACAAGGA GGGGCTCCGG GACTTCTACC TGGACCAGTT CCGCACCAAG
    GAGAACTCCC CGCACTGGGA GCACGTGGAC CTGACGGACA TCTACAAGGA GCAGAGCGAT
    GACTTTAAGC GCGACTCGGT GAGCGGAGGA GGGCCCTGTA CCAACAGGTC TCACCTCAAA
    CACGGGGCGG GCGACAAGCA CGGCGTGGTC AGCGGGGTCC CGGCCCCTTG GGAGAAGAAC
    CTGAGCAACG TGGATTGGGA GGACCGCTCC GGGGGCAACT TTTGCCGCAG CTGCCCCTCA
    AAGCTGCACA ACTACTCGAC GGCGGTGACC GGGCAGAACT CGGGCCGGCA GGCGTGCATC
    CGGTGCGAGG CCTGCAAGAA GGCAGGGAAC CTGTACGACA TCAGCGAGGA CAACTCCCTG
    CAGGAGCTGG ACCAGCCGGC TGCCCCTGTG GCGGTGCCGT CAAACGCGCC CAGCACCAAG
    TACCCCCAGA GCCCGACCAA CTCCAAGGCC CAGAAGAAGA CCCGGAACAA GCTGCGCCGG
    CAGCACTCCT ACGACACCTT CGTGGACCTG CAGAAGGAAG AAGCCGCCTT GGCCCCGCGC
    AGCGTGAGCC TGAAAGACAA GGGCCGGTTC CTGGACGGGA GTCCCTACGC CCACATGTTT
    GAGACGCCAG CTGGCGAGAG CACCTTTGCC AACCACGAGT CCTCAGTGGC CGCCGCCGGA
    CACCGCCACC ACAACAACCC CGGCGGCGGC GGCTACATGC TCAGCAAGTC GCTCTACCCC
    GACCGGGTCA CGCAAAACCC TTTCATCCCC ACTTTTGGGG ACGACCAGTG CTTGCTCCAT
    GGCAGCAAAT CCTACTTCTT CAGGCAGCCC ACGGTGGCGG GGGCGCCGAA AGCCAGGCCA
    GACTTCCGCG CCCTTGTCAC CAACAAGCCG GTGGTCTCGG CCCTGCACGG GGCCGTGCCA
    GGCCGTTTCC AGAAAGACAT CTGTATAGGG AACCAGTCCA ACCCCTGTGT GCCTAACAAC
    AAAAACCCCA GGGCTTTCAA TGGCTCCAGC AATGGGCATG TTTATGAGAA ACTTTCTAGT
    ATTGAGTCTG ATGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005636773.1
    CDS752..5209
    Translation

    Target ORF information:

    RefSeq Version XM_005636716.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris glutamate receptor, ionotropic, N-methyl D-aspartate 2B (GRIN2B), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005636716.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAGCCCA GAGCAGAGTG CTGTTCTCCC AAGTTCTGGT TGGTGTTGGC TGTCCTGGCT 
    GTGTCAGGCA GCAGAGCTCG TTCTCAGAAG AGCCCCCCCA GCATTGGCAT TGCTGTCATC
    CTCGTGGGCA CTTCAGACGA GGTGGCCATC AAGGATGCCC ACGAGAAAGA TGATTTCCAC
    CATCTCTCTG TGGTTCCCCG TGTGGAGCTG GTAGCCATGA ATGAGACTGA CCCAAAGAGC
    ATCATCACCC GCATCTGTGA TCTCATGTCT GACCGGAAGA TCCAGGGCGT GGTGTTTGCT
    GACGACACAG ATCAGGAGGC CATTGCCCAG ATCCTTGACT TCATTTCAGC CCAGACTCTC
    ACCCCCATCC TGGGCATCCA CGGAGGCTCC TCTATGATAA TGGCAGATAA GGATGAATCC
    TCCATGTTCT TCCAGTTTGG CCCATCAATT GAACAGCAAG CTTCTGTAAT GCTCAATATC
    ATGGAAGAGT ATGACTGGTA CATCTTTTCT ATTGTCACCA CCTACTTCCC TGGCTACCAG
    GACTTTGTAA ACAAGATTCG CAGCACCATT GAGAACAGCT TCGTGGGCTG GGAGTTAGAG
    GAAGTCCTCC TGCTGGACAT GTCCCTGGAT GATGGAGATT CTAAGATCCA GAACCAGCTC
    AAGAAACTGC AGAGCCCCAT CATTCTTCTC TATTGTACTA AGGAAGAAGC CACGTATATC
    TTTGAAGTAG CTAACTCAGT AGGGCTGACT GGCTACGGCT ACACATGGAT TGTACCTAGT
    CTGGTGGCAG GGGATACAGA CACAGTGCCC TCGGAGTTCC CCACTGGCCT CATTTCTGTG
    TCCTATGATG AATGGGACTA TGGCCTCCCT GCCAGAGTGA GGGATGGAAT TGCCATAATC
    ACCACTGCTG CTTCCGACAT GCTGTCTGAA CACAGCTTCA TCCCTGAGCC CAAGAGCAGT
    TGTTACAACA CCCATGAGAA GAGAATCTAC CAGTCCAACA TGCTAAATAG GTATCTGATC
    AATGTCACTT TTGAGGGAAG AAATCTGTCC TTCAGTGAAG ATGGCTACCA GATGCACCCG
    AAACTGGTGA TAATCCTTCT GAACAAGGAG AGAAAGTGGG AGAGGGTGGG GAAGTGGAAA
    GACAAATCCT TGCAGATGAA GTACTACGTG TGGCCCCGAA TGTGTCCTGA GACAGAAGAA
    CAGGAGGATG ACCATCTGAG CATTGTAACC CTGGAGGAGG CCCCCTTTGT CATTGTGGAG
    AGTGTGGACC CTCTGAGTGG AACCTGCATG AGGAACACAG TCCCCTGCCA AAAACGCATA
    GTCTCTGAGA ATAAAACAGA TGAAGAGCCA GGTTACATCA AAAAGTGCTG CAAGGGGTTC
    TGTATTGACA TCCTTAAGAA AATTTCTAAA TCTGTGAAGT TCACCTATGA TCTCTACCTG
    GTTACCAATG GCAAGCATGG GAAGAAAATC AATGGAACTT GGAATGGTAT GATTGGAGAG
    GTGGTTATGA AGAGGGCTTA CATGGCGGTG GGATCACTCA CCATCAATGA GGAGCGATCT
    GAGGTGGTTG ACTTCTCTGT GCCCTTCATA GAGACTGGCA TCAGTGTTAT GGTGTCACGC
    AGCAATGGGA CTGTCTCACC ATCTGCCTTC TTAGAGCCAT TCAGTGCTGA CGTATGGGTT
    ATGATGTTCG TGATGCTGCT CATCGTCTCA GCCGTGGCTG TCTTTGTCTT CGAGTACTTC
    AGCCCTGTGG GTTACAACAG GTGCCTCGCT GATGGCAGAG AGCCAGGTGG CCCGTCTTTT
    ACCATCGGCA AAGCTATTTG GTTACTGTGG GGTCTGGTGT TTAACAACTC CGTACCCGTG
    CAGAACCCAA AGGGGACCAC TTCCAAGATC ATGGTGTCGG TGTGGGCCTT CTTTGCCGTC
    ATCTTCCTGG CCAGCTACAC TGCCAACTTA GCTGCCTTCA TGATCCAAGA AGAGTATGTG
    GACCAGGTTT CTGGCCTGAG TGACAAAAAG TTCCAGAGAC CTAATGACTT CTCACCCCCT
    TTTCGCTTTG GGACTGTGCC CAATGGCAGC ACAGAGAGGA ATATTCGCAA TAACTACGCA
    GAAATGCATG CCTACATGGG AAAGTTCAAC CAGAGGGGTG TAGATGATGC ATTGCTCTCC
    CTGAAAACAG GGAAACTGGA TGCCTTCATC TATGATGCAG CAGTGCTGAA CTACATGGCG
    GGCAGAGATG AAGGCTGCAA GCTGGTGACC ATTGGCAGTG GGAAAGTCTT TGCTTCCACT
    GGTTATGGCA TTGCCATCCA AAAAGATTCT GGGTGGAAGC GCCAGGTGGA CCTTGCTATC
    CTCCAGCTAT TCGGAGATGG GGAGATGGAG GAGCTAGAAG CTCTTTGGCT CACTGGCATT
    TGCCACAATG AAAAGAATGA GGTCATGAGC AGCCAGCTGG ACATTGACAA CATGGCAGGA
    GTCTTCTACA TGTTGGGGGC AGCCATGGCT CTCAGTCTCA TCACCTTCAT CTGTGAACAC
    CTTTTCTATT GGCAGTTCCG GCACTGCTTT ATGGGTGTCT GTTCCGGCAA GCCTGGCATG
    GTCTTCTCCA TCAGCAGAGG TATCTACAGT TGCATCCATG GAGTGGCTAT CGAGGAGCGC
    CAGTCCGTGA TGAATTCCCC CACCGCGACC ATGAACAACA CACACTCCAA CATCCTGCGC
    CTGCTCCGCA CGGCCAAGAA CATGGCCAAC CTGTCTGGTG TGAACGGCTC CCCCCAGAGT
    GCCCTGGACT TCATCCGACG TGAGTCGTCC GTCTATGACA TCTCCGAGCA CCGCCGCAGC
    TTCACGCATT CCGACTGCAA ATCCTACAAC AACCCGCCGT GCGAGGAGAA CCTCTTCAGC
    GACTACATCA GTGAGGTGGA GAGGACTTTT GGTAACCTGC AGCTGAAGGA CAGCAACGTG
    TACCAAGATC ACTATCACCA CCACCACCGG CCCCACAGCA TCGGCAGCGC CAGCTCCATC
    GACGGGCTCT ACGACTGTGA CAACCCGCCT TTCACCACCC AGCCGAGGTC CATCAGCAAG
    AAGCCCCTGG ACCTCGGCCT CCCCTCCTCC AAACACAGCC AGCTCAGTGA CCTGTACGGC
    AAATTCTCCT TCAAGAGCGA CCGCTACAGT GGCCACGATG ACTTGATCCG CTCGGACGTG
    TCTGACATCT CCACGCACAC GGTCACCTAC GGAAACATAG AGGGCAACGC CGCCAAGAGG
    CGCAAGCAGC AATACAAGGA CAGCCTGAAG AAGCGGCCGG CCTCGGCCAA GTCCCGGCGG
    GAGTTCGACG AGATCGAGCT GGCCTACCGC CGCCGGCCGC CCCGCTCCCC TGACCACAAG
    CGCTACTTCA GGGACAAGGA GGGGCTCCGG GACTTCTACC TGGACCAGTT CCGCACCAAG
    GAGAACTCCC CGCACTGGGA GCACGTGGAC CTGACGGACA TCTACAAGGA GCAGAGCGAT
    GACTTTAAGC GCGACTCGGT GAGCGGAGGA GGGCCCTGTA CCAACAGGTC TCACCTCAAA
    CACGGGGCGG GCGACAAGCA CGGCGTGGTC AGCGGGGTCC CGGCCCCTTG GGAGAAGAAC
    CTGAGCAACG TGGATTGGGA GGACCGCTCC GGGGGCAACT TTTGCCGCAG CTGCCCCTCA
    AAGCTGCACA ACTACTCGAC GGCGGTGACC GGGCAGAACT CGGGCCGGCA GGCGTGCATC
    CGGTGCGAGG CCTGCAAGAA GGCAGGGAAC CTGTACGACA TCAGCGAGGA CAACTCCCTG
    CAGGAGCTGG ACCAGCCGGC TGCCCCTGTG GCGGTGCCGT CAAACGCGCC CAGCACCAAG
    TACCCCCAGA GCCCGACCAA CTCCAAGGCC CAGAAGAAGA CCCGGAACAA GCTGCGCCGG
    CAGCACTCCT ACGACACCTT CGTGGACCTG CAGAAGGAAG AAGCCGCCTT GGCCCCGCGC
    AGCGTGAGCC TGAAAGACAA GGGCCGGTTC CTGGACGGGA GTCCCTACGC CCACATGTTT
    GAGACGCCAG CTGGCGAGAG CACCTTTGCC AACCACGAGT CCTCAGTGGC CGCCGCCGGA
    CACCGCCACC ACAACAACCC CGGCGGCGGC GGCTACATGC TCAGCAAGTC GCTCTACCCC
    GACCGGGTCA CGCAAAACCC TTTCATCCCC ACTTTTGGGG ACGACCAGTG CTTGCTCCAT
    GGCAGCAAAT CCTACTTCTT CAGGCAGCCC ACGGTGGCGG GGGCGCCGAA AGCCAGGCCA
    GACTTCCGCG CCCTTGTCAC CAACAAGCCG GTGGTCTCGG CCCTGCACGG GGCCGTGCCA
    GGCCGTTTCC AGAAAGACAT CTGTATAGGG AACCAGTCCA ACCCCTGTGT GCCTAACAAC
    AAAAACCCCA GGGCTTTCAA TGGCTCCAGC AATGGGCATG TTTATGAGAA ACTTTCTAGT
    ATTGAGTCTG ATGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26829
    Clone ID Related Accession (Same CDS sequence) XM_022410866.1 , XM_014108268.1 , XM_005636716.2 , XM_014108267.1 , NM_001008719.1
    Accession Version XM_014108267.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4458bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product glutamate receptor ionotropic, NMDA 2B isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAGCCCA GAGCAGAGTG CTGTTCTCCC AAGTTCTGGT TGGTGTTGGC TGTCCTGGCT 
    GTGTCAGGCA GCAGAGCTCG TTCTCAGAAG AGCCCCCCCA GCATTGGCAT TGCTGTCATC
    CTCGTGGGCA CTTCAGACGA GGTGGCCATC AAGGATGCCC ACGAGAAAGA TGATTTCCAC
    CATCTCTCTG TGGTTCCCCG TGTGGAGCTG GTAGCCATGA ATGAGACTGA CCCAAAGAGC
    ATCATCACCC GCATCTGTGA TCTCATGTCT GACCGGAAGA TCCAGGGCGT GGTGTTTGCT
    GACGACACAG ATCAGGAGGC CATTGCCCAG ATCCTTGACT TCATTTCAGC CCAGACTCTC
    ACCCCCATCC TGGGCATCCA CGGAGGCTCC TCTATGATAA TGGCAGATAA GGATGAATCC
    TCCATGTTCT TCCAGTTTGG CCCATCAATT GAACAGCAAG CTTCTGTAAT GCTCAATATC
    ATGGAAGAGT ATGACTGGTA CATCTTTTCT ATTGTCACCA CCTACTTCCC TGGCTACCAG
    GACTTTGTAA ACAAGATTCG CAGCACCATT GAGAACAGCT TCGTGGGCTG GGAGTTAGAG
    GAAGTCCTCC TGCTGGACAT GTCCCTGGAT GATGGAGATT CTAAGATCCA GAACCAGCTC
    AAGAAACTGC AGAGCCCCAT CATTCTTCTC TATTGTACTA AGGAAGAAGC CACGTATATC
    TTTGAAGTAG CTAACTCAGT AGGGCTGACT GGCTACGGCT ACACATGGAT TGTACCTAGT
    CTGGTGGCAG GGGATACAGA CACAGTGCCC TCGGAGTTCC CCACTGGCCT CATTTCTGTG
    TCCTATGATG AATGGGACTA TGGCCTCCCT GCCAGAGTGA GGGATGGAAT TGCCATAATC
    ACCACTGCTG CTTCCGACAT GCTGTCTGAA CACAGCTTCA TCCCTGAGCC CAAGAGCAGT
    TGTTACAACA CCCATGAGAA GAGAATCTAC CAGTCCAACA TGCTAAATAG GTATCTGATC
    AATGTCACTT TTGAGGGAAG AAATCTGTCC TTCAGTGAAG ATGGCTACCA GATGCACCCG
    AAACTGGTGA TAATCCTTCT GAACAAGGAG AGAAAGTGGG AGAGGGTGGG GAAGTGGAAA
    GACAAATCCT TGCAGATGAA GTACTACGTG TGGCCCCGAA TGTGTCCTGA GACAGAAGAA
    CAGGAGGATG ACCATCTGAG CATTGTAACC CTGGAGGAGG CCCCCTTTGT CATTGTGGAG
    AGTGTGGACC CTCTGAGTGG AACCTGCATG AGGAACACAG TCCCCTGCCA AAAACGCATA
    GTCTCTGAGA ATAAAACAGA TGAAGAGCCA GGTTACATCA AAAAGTGCTG CAAGGGGTTC
    TGTATTGACA TCCTTAAGAA AATTTCTAAA TCTGTGAAGT TCACCTATGA TCTCTACCTG
    GTTACCAATG GCAAGCATGG GAAGAAAATC AATGGAACTT GGAATGGTAT GATTGGAGAG
    GTGGTTATGA AGAGGGCTTA CATGGCGGTG GGATCACTCA CCATCAATGA GGAGCGATCT
    GAGGTGGTTG ACTTCTCTGT GCCCTTCATA GAGACTGGCA TCAGTGTTAT GGTGTCACGC
    AGCAATGGGA CTGTCTCACC ATCTGCCTTC TTAGAGCCAT TCAGTGCTGA CGTATGGGTT
    ATGATGTTCG TGATGCTGCT CATCGTCTCA GCCGTGGCTG TCTTTGTCTT CGAGTACTTC
    AGCCCTGTGG GTTACAACAG GTGCCTCGCT GATGGCAGAG AGCCAGGTGG CCCGTCTTTT
    ACCATCGGCA AAGCTATTTG GTTACTGTGG GGTCTGGTGT TTAACAACTC CGTACCCGTG
    CAGAACCCAA AGGGGACCAC TTCCAAGATC ATGGTGTCGG TGTGGGCCTT CTTTGCCGTC
    ATCTTCCTGG CCAGCTACAC TGCCAACTTA GCTGCCTTCA TGATCCAAGA AGAGTATGTG
    GACCAGGTTT CTGGCCTGAG TGACAAAAAG TTCCAGAGAC CTAATGACTT CTCACCCCCT
    TTTCGCTTTG GGACTGTGCC CAATGGCAGC ACAGAGAGGA ATATTCGCAA TAACTACGCA
    GAAATGCATG CCTACATGGG AAAGTTCAAC CAGAGGGGTG TAGATGATGC ATTGCTCTCC
    CTGAAAACAG GGAAACTGGA TGCCTTCATC TATGATGCAG CAGTGCTGAA CTACATGGCG
    GGCAGAGATG AAGGCTGCAA GCTGGTGACC ATTGGCAGTG GGAAAGTCTT TGCTTCCACT
    GGTTATGGCA TTGCCATCCA AAAAGATTCT GGGTGGAAGC GCCAGGTGGA CCTTGCTATC
    CTCCAGCTAT TCGGAGATGG GGAGATGGAG GAGCTAGAAG CTCTTTGGCT CACTGGCATT
    TGCCACAATG AAAAGAATGA GGTCATGAGC AGCCAGCTGG ACATTGACAA CATGGCAGGA
    GTCTTCTACA TGTTGGGGGC AGCCATGGCT CTCAGTCTCA TCACCTTCAT CTGTGAACAC
    CTTTTCTATT GGCAGTTCCG GCACTGCTTT ATGGGTGTCT GTTCCGGCAA GCCTGGCATG
    GTCTTCTCCA TCAGCAGAGG TATCTACAGT TGCATCCATG GAGTGGCTAT CGAGGAGCGC
    CAGTCCGTGA TGAATTCCCC CACCGCGACC ATGAACAACA CACACTCCAA CATCCTGCGC
    CTGCTCCGCA CGGCCAAGAA CATGGCCAAC CTGTCTGGTG TGAACGGCTC CCCCCAGAGT
    GCCCTGGACT TCATCCGACG TGAGTCGTCC GTCTATGACA TCTCCGAGCA CCGCCGCAGC
    TTCACGCATT CCGACTGCAA ATCCTACAAC AACCCGCCGT GCGAGGAGAA CCTCTTCAGC
    GACTACATCA GTGAGGTGGA GAGGACTTTT GGTAACCTGC AGCTGAAGGA CAGCAACGTG
    TACCAAGATC ACTATCACCA CCACCACCGG CCCCACAGCA TCGGCAGCGC CAGCTCCATC
    GACGGGCTCT ACGACTGTGA CAACCCGCCT TTCACCACCC AGCCGAGGTC CATCAGCAAG
    AAGCCCCTGG ACCTCGGCCT CCCCTCCTCC AAACACAGCC AGCTCAGTGA CCTGTACGGC
    AAATTCTCCT TCAAGAGCGA CCGCTACAGT GGCCACGATG ACTTGATCCG CTCGGACGTG
    TCTGACATCT CCACGCACAC GGTCACCTAC GGAAACATAG AGGGCAACGC CGCCAAGAGG
    CGCAAGCAGC AATACAAGGA CAGCCTGAAG AAGCGGCCGG CCTCGGCCAA GTCCCGGCGG
    GAGTTCGACG AGATCGAGCT GGCCTACCGC CGCCGGCCGC CCCGCTCCCC TGACCACAAG
    CGCTACTTCA GGGACAAGGA GGGGCTCCGG GACTTCTACC TGGACCAGTT CCGCACCAAG
    GAGAACTCCC CGCACTGGGA GCACGTGGAC CTGACGGACA TCTACAAGGA GCAGAGCGAT
    GACTTTAAGC GCGACTCGGT GAGCGGAGGA GGGCCCTGTA CCAACAGGTC TCACCTCAAA
    CACGGGGCGG GCGACAAGCA CGGCGTGGTC AGCGGGGTCC CGGCCCCTTG GGAGAAGAAC
    CTGAGCAACG TGGATTGGGA GGACCGCTCC GGGGGCAACT TTTGCCGCAG CTGCCCCTCA
    AAGCTGCACA ACTACTCGAC GGCGGTGACC GGGCAGAACT CGGGCCGGCA GGCGTGCATC
    CGGTGCGAGG CCTGCAAGAA GGCAGGGAAC CTGTACGACA TCAGCGAGGA CAACTCCCTG
    CAGGAGCTGG ACCAGCCGGC TGCCCCTGTG GCGGTGCCGT CAAACGCGCC CAGCACCAAG
    TACCCCCAGA GCCCGACCAA CTCCAAGGCC CAGAAGAAGA CCCGGAACAA GCTGCGCCGG
    CAGCACTCCT ACGACACCTT CGTGGACCTG CAGAAGGAAG AAGCCGCCTT GGCCCCGCGC
    AGCGTGAGCC TGAAAGACAA GGGCCGGTTC CTGGACGGGA GTCCCTACGC CCACATGTTT
    GAGACGCCAG CTGGCGAGAG CACCTTTGCC AACCACGAGT CCTCAGTGGC CGCCGCCGGA
    CACCGCCACC ACAACAACCC CGGCGGCGGC GGCTACATGC TCAGCAAGTC GCTCTACCCC
    GACCGGGTCA CGCAAAACCC TTTCATCCCC ACTTTTGGGG ACGACCAGTG CTTGCTCCAT
    GGCAGCAAAT CCTACTTCTT CAGGCAGCCC ACGGTGGCGG GGGCGCCGAA AGCCAGGCCA
    GACTTCCGCG CCCTTGTCAC CAACAAGCCG GTGGTCTCGG CCCTGCACGG GGCCGTGCCA
    GGCCGTTTCC AGAAAGACAT CTGTATAGGG AACCAGTCCA ACCCCTGTGT GCCTAACAAC
    AAAAACCCCA GGGCTTTCAA TGGCTCCAGC AATGGGCATG TTTATGAGAA ACTTTCTAGT
    ATTGAGTCTG ATGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963742.1
    CDS779..5236
    Translation

    Target ORF information:

    RefSeq Version XM_014108267.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris glutamate receptor, ionotropic, N-methyl D-aspartate 2B (GRIN2B), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014108267.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAGCCCA GAGCAGAGTG CTGTTCTCCC AAGTTCTGGT TGGTGTTGGC TGTCCTGGCT 
    GTGTCAGGCA GCAGAGCTCG TTCTCAGAAG AGCCCCCCCA GCATTGGCAT TGCTGTCATC
    CTCGTGGGCA CTTCAGACGA GGTGGCCATC AAGGATGCCC ACGAGAAAGA TGATTTCCAC
    CATCTCTCTG TGGTTCCCCG TGTGGAGCTG GTAGCCATGA ATGAGACTGA CCCAAAGAGC
    ATCATCACCC GCATCTGTGA TCTCATGTCT GACCGGAAGA TCCAGGGCGT GGTGTTTGCT
    GACGACACAG ATCAGGAGGC CATTGCCCAG ATCCTTGACT TCATTTCAGC CCAGACTCTC
    ACCCCCATCC TGGGCATCCA CGGAGGCTCC TCTATGATAA TGGCAGATAA GGATGAATCC
    TCCATGTTCT TCCAGTTTGG CCCATCAATT GAACAGCAAG CTTCTGTAAT GCTCAATATC
    ATGGAAGAGT ATGACTGGTA CATCTTTTCT ATTGTCACCA CCTACTTCCC TGGCTACCAG
    GACTTTGTAA ACAAGATTCG CAGCACCATT GAGAACAGCT TCGTGGGCTG GGAGTTAGAG
    GAAGTCCTCC TGCTGGACAT GTCCCTGGAT GATGGAGATT CTAAGATCCA GAACCAGCTC
    AAGAAACTGC AGAGCCCCAT CATTCTTCTC TATTGTACTA AGGAAGAAGC CACGTATATC
    TTTGAAGTAG CTAACTCAGT AGGGCTGACT GGCTACGGCT ACACATGGAT TGTACCTAGT
    CTGGTGGCAG GGGATACAGA CACAGTGCCC TCGGAGTTCC CCACTGGCCT CATTTCTGTG
    TCCTATGATG AATGGGACTA TGGCCTCCCT GCCAGAGTGA GGGATGGAAT TGCCATAATC
    ACCACTGCTG CTTCCGACAT GCTGTCTGAA CACAGCTTCA TCCCTGAGCC CAAGAGCAGT
    TGTTACAACA CCCATGAGAA GAGAATCTAC CAGTCCAACA TGCTAAATAG GTATCTGATC
    AATGTCACTT TTGAGGGAAG AAATCTGTCC TTCAGTGAAG ATGGCTACCA GATGCACCCG
    AAACTGGTGA TAATCCTTCT GAACAAGGAG AGAAAGTGGG AGAGGGTGGG GAAGTGGAAA
    GACAAATCCT TGCAGATGAA GTACTACGTG TGGCCCCGAA TGTGTCCTGA GACAGAAGAA
    CAGGAGGATG ACCATCTGAG CATTGTAACC CTGGAGGAGG CCCCCTTTGT CATTGTGGAG
    AGTGTGGACC CTCTGAGTGG AACCTGCATG AGGAACACAG TCCCCTGCCA AAAACGCATA
    GTCTCTGAGA ATAAAACAGA TGAAGAGCCA GGTTACATCA AAAAGTGCTG CAAGGGGTTC
    TGTATTGACA TCCTTAAGAA AATTTCTAAA TCTGTGAAGT TCACCTATGA TCTCTACCTG
    GTTACCAATG GCAAGCATGG GAAGAAAATC AATGGAACTT GGAATGGTAT GATTGGAGAG
    GTGGTTATGA AGAGGGCTTA CATGGCGGTG GGATCACTCA CCATCAATGA GGAGCGATCT
    GAGGTGGTTG ACTTCTCTGT GCCCTTCATA GAGACTGGCA TCAGTGTTAT GGTGTCACGC
    AGCAATGGGA CTGTCTCACC ATCTGCCTTC TTAGAGCCAT TCAGTGCTGA CGTATGGGTT
    ATGATGTTCG TGATGCTGCT CATCGTCTCA GCCGTGGCTG TCTTTGTCTT CGAGTACTTC
    AGCCCTGTGG GTTACAACAG GTGCCTCGCT GATGGCAGAG AGCCAGGTGG CCCGTCTTTT
    ACCATCGGCA AAGCTATTTG GTTACTGTGG GGTCTGGTGT TTAACAACTC CGTACCCGTG
    CAGAACCCAA AGGGGACCAC TTCCAAGATC ATGGTGTCGG TGTGGGCCTT CTTTGCCGTC
    ATCTTCCTGG CCAGCTACAC TGCCAACTTA GCTGCCTTCA TGATCCAAGA AGAGTATGTG
    GACCAGGTTT CTGGCCTGAG TGACAAAAAG TTCCAGAGAC CTAATGACTT CTCACCCCCT
    TTTCGCTTTG GGACTGTGCC CAATGGCAGC ACAGAGAGGA ATATTCGCAA TAACTACGCA
    GAAATGCATG CCTACATGGG AAAGTTCAAC CAGAGGGGTG TAGATGATGC ATTGCTCTCC
    CTGAAAACAG GGAAACTGGA TGCCTTCATC TATGATGCAG CAGTGCTGAA CTACATGGCG
    GGCAGAGATG AAGGCTGCAA GCTGGTGACC ATTGGCAGTG GGAAAGTCTT TGCTTCCACT
    GGTTATGGCA TTGCCATCCA AAAAGATTCT GGGTGGAAGC GCCAGGTGGA CCTTGCTATC
    CTCCAGCTAT TCGGAGATGG GGAGATGGAG GAGCTAGAAG CTCTTTGGCT CACTGGCATT
    TGCCACAATG AAAAGAATGA GGTCATGAGC AGCCAGCTGG ACATTGACAA CATGGCAGGA
    GTCTTCTACA TGTTGGGGGC AGCCATGGCT CTCAGTCTCA TCACCTTCAT CTGTGAACAC
    CTTTTCTATT GGCAGTTCCG GCACTGCTTT ATGGGTGTCT GTTCCGGCAA GCCTGGCATG
    GTCTTCTCCA TCAGCAGAGG TATCTACAGT TGCATCCATG GAGTGGCTAT CGAGGAGCGC
    CAGTCCGTGA TGAATTCCCC CACCGCGACC ATGAACAACA CACACTCCAA CATCCTGCGC
    CTGCTCCGCA CGGCCAAGAA CATGGCCAAC CTGTCTGGTG TGAACGGCTC CCCCCAGAGT
    GCCCTGGACT TCATCCGACG TGAGTCGTCC GTCTATGACA TCTCCGAGCA CCGCCGCAGC
    TTCACGCATT CCGACTGCAA ATCCTACAAC AACCCGCCGT GCGAGGAGAA CCTCTTCAGC
    GACTACATCA GTGAGGTGGA GAGGACTTTT GGTAACCTGC AGCTGAAGGA CAGCAACGTG
    TACCAAGATC ACTATCACCA CCACCACCGG CCCCACAGCA TCGGCAGCGC CAGCTCCATC
    GACGGGCTCT ACGACTGTGA CAACCCGCCT TTCACCACCC AGCCGAGGTC CATCAGCAAG
    AAGCCCCTGG ACCTCGGCCT CCCCTCCTCC AAACACAGCC AGCTCAGTGA CCTGTACGGC
    AAATTCTCCT TCAAGAGCGA CCGCTACAGT GGCCACGATG ACTTGATCCG CTCGGACGTG
    TCTGACATCT CCACGCACAC GGTCACCTAC GGAAACATAG AGGGCAACGC CGCCAAGAGG
    CGCAAGCAGC AATACAAGGA CAGCCTGAAG AAGCGGCCGG CCTCGGCCAA GTCCCGGCGG
    GAGTTCGACG AGATCGAGCT GGCCTACCGC CGCCGGCCGC CCCGCTCCCC TGACCACAAG
    CGCTACTTCA GGGACAAGGA GGGGCTCCGG GACTTCTACC TGGACCAGTT CCGCACCAAG
    GAGAACTCCC CGCACTGGGA GCACGTGGAC CTGACGGACA TCTACAAGGA GCAGAGCGAT
    GACTTTAAGC GCGACTCGGT GAGCGGAGGA GGGCCCTGTA CCAACAGGTC TCACCTCAAA
    CACGGGGCGG GCGACAAGCA CGGCGTGGTC AGCGGGGTCC CGGCCCCTTG GGAGAAGAAC
    CTGAGCAACG TGGATTGGGA GGACCGCTCC GGGGGCAACT TTTGCCGCAG CTGCCCCTCA
    AAGCTGCACA ACTACTCGAC GGCGGTGACC GGGCAGAACT CGGGCCGGCA GGCGTGCATC
    CGGTGCGAGG CCTGCAAGAA GGCAGGGAAC CTGTACGACA TCAGCGAGGA CAACTCCCTG
    CAGGAGCTGG ACCAGCCGGC TGCCCCTGTG GCGGTGCCGT CAAACGCGCC CAGCACCAAG
    TACCCCCAGA GCCCGACCAA CTCCAAGGCC CAGAAGAAGA CCCGGAACAA GCTGCGCCGG
    CAGCACTCCT ACGACACCTT CGTGGACCTG CAGAAGGAAG AAGCCGCCTT GGCCCCGCGC
    AGCGTGAGCC TGAAAGACAA GGGCCGGTTC CTGGACGGGA GTCCCTACGC CCACATGTTT
    GAGACGCCAG CTGGCGAGAG CACCTTTGCC AACCACGAGT CCTCAGTGGC CGCCGCCGGA
    CACCGCCACC ACAACAACCC CGGCGGCGGC GGCTACATGC TCAGCAAGTC GCTCTACCCC
    GACCGGGTCA CGCAAAACCC TTTCATCCCC ACTTTTGGGG ACGACCAGTG CTTGCTCCAT
    GGCAGCAAAT CCTACTTCTT CAGGCAGCCC ACGGTGGCGG GGGCGCCGAA AGCCAGGCCA
    GACTTCCGCG CCCTTGTCAC CAACAAGCCG GTGGTCTCGG CCCTGCACGG GGCCGTGCCA
    GGCCGTTTCC AGAAAGACAT CTGTATAGGG AACCAGTCCA ACCCCTGTGT GCCTAACAAC
    AAAAACCCCA GGGCTTTCAA TGGCTCCAGC AATGGGCATG TTTATGAGAA ACTTTCTAGT
    ATTGAGTCTG ATGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26830
    Clone ID Related Accession (Same CDS sequence) XM_022410867.1 , XM_022410868.1
    Accession Version XM_022410867.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4119bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product glutamate receptor ionotropic, NMDA 2B isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTTCTTCC AGTTTGGCCC ATCAATTGAA CAGCAAGCTT CTGTAATGCT CAATATCATG 
    GAAGAGTATG ACTGGTACAT CTTTTCTATT GTCACCACCT ACTTCCCTGG CTACCAGGAC
    TTTGTAAACA AGATTCGCAG CACCATTGAG AACAGCTTCG TGGGCTGGGA GTTAGAGGAA
    GTCCTCCTGC TGGACATGTC CCTGGATGAT GGAGATTCTA AGATCCAGAA CCAGCTCAAG
    AAACTGCAGA GCCCCATCAT TCTTCTCTAT TGTACTAAGG AAGAAGCCAC GTATATCTTT
    GAAGTAGCTA ACTCAGTAGG GCTGACTGGC TACGGCTACA CATGGATTGT ACCTAGTCTG
    GTGGCAGGGG ATACAGACAC AGTGCCCTCG GAGTTCCCCA CTGGCCTCAT TTCTGTGTCC
    TATGATGAAT GGGACTATGG CCTCCCTGCC AGAGTGAGGG ATGGAATTGC CATAATCACC
    ACTGCTGCTT CCGACATGCT GTCTGAACAC AGCTTCATCC CTGAGCCCAA GAGCAGTTGT
    TACAACACCC ATGAGAAGAG AATCTACCAG TCCAACATGC TAAATAGATG CAGAGACAGT
    AAAGATAACA AGATTTGGAG AAAAAAGAAT AACCAAAAGA AGGGACCATT TCACAGGGCT
    GTTGATTTTG TGTATCTGAT CAATGTCACT TTTGAGGGAA GAAATCTGTC CTTCAGTGAA
    GATGGCTACC AGATGCACCC GAAACTGGTG ATAATCCTTC TGAACAAGGA GAGAAAGTGG
    GAGAGGGTGG GGAAGTGGAA AGACAAATCC TTGCAGATGA AGTACTACGT GTGGCCCCGA
    ATGTGTCCTG AGACAGAAGA ACAGGAGGAT GACCATCTGA GCATTGTAAC CCTGGAGGAG
    GCCCCCTTTG TCATTGTGGA GAGTGTGGAC CCTCTGAGTG GAACCTGCAT GAGGAACACA
    GTCCCCTGCC AAAAACGCAT AGTCTCTGAG AATAAAACAG ATGAAGAGCC AGGTTACATC
    AAAAAGTGCT GCAAGGGGTT CTGTATTGAC ATCCTTAAGA AAATTTCTAA ATCTGTGAAG
    TTCACCTATG ATCTCTACCT GGTTACCAAT GGCAAGCATG GGAAGAAAAT CAATGGAACT
    TGGAATGGTA TGATTGGAGA GGTGGTTATG AAGAGGGCTT ACATGGCGGT GGGATCACTC
    ACCATCAATG AGGAGCGATC TGAGGTGGTT GACTTCTCTG TGCCCTTCAT AGAGACTGGC
    ATCAGTGTTA TGGTGTCACG CAGCAATGGG ACTGTCTCAC CATCTGCCTT CTTAGAGCCA
    TTCAGTGCTG ACGTATGGGT TATGATGTTC GTGATGCTGC TCATCGTCTC AGCCGTGGCT
    GTCTTTGTCT TCGAGTACTT CAGCCCTGTG GGTTACAACA GGTGCCTCGC TGATGGCAGA
    GAGCCAGGTG GCCCGTCTTT TACCATCGGC AAAGCTATTT GGTTACTGTG GGGTCTGGTG
    TTTAACAACT CCGTACCCGT GCAGAACCCA AAGGGGACCA CTTCCAAGAT CATGGTGTCG
    GTGTGGGCCT TCTTTGCCGT CATCTTCCTG GCCAGCTACA CTGCCAACTT AGCTGCCTTC
    ATGATCCAAG AAGAGTATGT GGACCAGGTT TCTGGCCTGA GTGACAAAAA GTTCCAGAGA
    CCTAATGACT TCTCACCCCC TTTTCGCTTT GGGACTGTGC CCAATGGCAG CACAGAGAGG
    AATATTCGCA ATAACTACGC AGAAATGCAT GCCTACATGG GAAAGTTCAA CCAGAGGGGT
    GTAGATGATG CATTGCTCTC CCTGAAAACA GGGAAACTGG ATGCCTTCAT CTATGATGCA
    GCAGTGCTGA ACTACATGGC GGGCAGAGAT GAAGGCTGCA AGCTGGTGAC CATTGGCAGT
    GGGAAAGTCT TTGCTTCCAC TGGTTATGGC ATTGCCATCC AAAAAGATTC TGGGTGGAAG
    CGCCAGGTGG ACCTTGCTAT CCTCCAGCTA TTCGGAGATG GGGAGATGGA GGAGCTAGAA
    GCTCTTTGGC TCACTGGCAT TTGCCACAAT GAAAAGAATG AGGTCATGAG CAGCCAGCTG
    GACATTGACA ACATGGCAGG AGTCTTCTAC ATGTTGGGGG CAGCCATGGC TCTCAGTCTC
    ATCACCTTCA TCTGTGAACA CCTTTTCTAT TGGCAGTTCC GGCACTGCTT TATGGGTGTC
    TGTTCCGGCA AGCCTGGCAT GGTCTTCTCC ATCAGCAGAG GTATCTACAG TTGCATCCAT
    GGAGTGGCTA TCGAGGAGCG CCAGTCCGTG ATGAATTCCC CCACCGCGAC CATGAACAAC
    ACACACTCCA ACATCCTGCG CCTGCTCCGC ACGGCCAAGA ACATGGCCAA CCTGTCTGGT
    GTGAACGGCT CCCCCCAGAG TGCCCTGGAC TTCATCCGAC GTGAGTCGTC CGTCTATGAC
    ATCTCCGAGC ACCGCCGCAG CTTCACGCAT TCCGACTGCA AATCCTACAA CAACCCGCCG
    TGCGAGGAGA ACCTCTTCAG CGACTACATC AGTGAGGTGG AGAGGACTTT TGGTAACCTG
    CAGCTGAAGG ACAGCAACGT GTACCAAGAT CACTATCACC ACCACCACCG GCCCCACAGC
    ATCGGCAGCG CCAGCTCCAT CGACGGGCTC TACGACTGTG ACAACCCGCC TTTCACCACC
    CAGCCGAGGT CCATCAGCAA GAAGCCCCTG GACCTCGGCC TCCCCTCCTC CAAACACAGC
    CAGCTCAGTG ACCTGTACGG CAAATTCTCC TTCAAGAGCG ACCGCTACAG TGGCCACGAT
    GACTTGATCC GCTCGGACGT GTCTGACATC TCCACGCACA CGGTCACCTA CGGAAACATA
    GAGGGCAACG CCGCCAAGAG GCGCAAGCAG CAATACAAGG ACAGCCTGAA GAAGCGGCCG
    GCCTCGGCCA AGTCCCGGCG GGAGTTCGAC GAGATCGAGC TGGCCTACCG CCGCCGGCCG
    CCCCGCTCCC CTGACCACAA GCGCTACTTC AGGGACAAGG AGGGGCTCCG GGACTTCTAC
    CTGGACCAGT TCCGCACCAA GGAGAACTCC CCGCACTGGG AGCACGTGGA CCTGACGGAC
    ATCTACAAGG AGCAGAGCGA TGACTTTAAG CGCGACTCGG TGAGCGGAGG AGGGCCCTGT
    ACCAACAGGT CTCACCTCAA ACACGGGGCG GGCGACAAGC ACGGCGTGGT CAGCGGGGTC
    CCGGCCCCTT GGGAGAAGAA CCTGAGCAAC GTGGATTGGG AGGACCGCTC CGGGGGCAAC
    TTTTGCCGCA GCTGCCCCTC AAAGCTGCAC AACTACTCGA CGGCGGTGAC CGGGCAGAAC
    TCGGGCCGGC AGGCGTGCAT CCGGTGCGAG GCCTGCAAGA AGGCAGGGAA CCTGTACGAC
    ATCAGCGAGG ACAACTCCCT GCAGGAGCTG GACCAGCCGG CTGCCCCTGT GGCGGTGCCG
    TCAAACGCGC CCAGCACCAA GTACCCCCAG AGCCCGACCA ACTCCAAGGC CCAGAAGAAG
    ACCCGGAACA AGCTGCGCCG GCAGCACTCC TACGACACCT TCGTGGACCT GCAGAAGGAA
    GAAGCCGCCT TGGCCCCGCG CAGCGTGAGC CTGAAAGACA AGGGCCGGTT CCTGGACGGG
    AGTCCCTACG CCCACATGTT TGAGACGCCA GCTGGCGAGA GCACCTTTGC CAACCACGAG
    TCCTCAGTGG CCGCCGCCGG ACACCGCCAC CACAACAACC CCGGCGGCGG CGGCTACATG
    CTCAGCAAGT CGCTCTACCC CGACCGGGTC ACGCAAAACC CTTTCATCCC CACTTTTGGG
    GACGACCAGT GCTTGCTCCA TGGCAGCAAA TCCTACTTCT TCAGGCAGCC CACGGTGGCG
    GGGGCGCCGA AAGCCAGGCC AGACTTCCGC GCCCTTGTCA CCAACAAGCC GGTGGTCTCG
    GCCCTGCACG GGGCCGTGCC AGGCCGTTTC CAGAAAGACA TCTGTATAGG GAACCAGTCC
    AACCCCTGTG TGCCTAACAA CAAAAACCCC AGGGCTTTCA ATGGCTCCAG CAATGGGCAT
    GTTTATGAGA AACTTTCTAG TATTGAGTCT GATGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022266575.1
    CDS145..4263
    Translation

    Target ORF information:

    RefSeq Version XM_022410867.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris glutamate ionotropic receptor NMDA type subunit 2B (GRIN2B), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022410867.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTTCTTCC AGTTTGGCCC ATCAATTGAA CAGCAAGCTT CTGTAATGCT CAATATCATG 
    GAAGAGTATG ACTGGTACAT CTTTTCTATT GTCACCACCT ACTTCCCTGG CTACCAGGAC
    TTTGTAAACA AGATTCGCAG CACCATTGAG AACAGCTTCG TGGGCTGGGA GTTAGAGGAA
    GTCCTCCTGC TGGACATGTC CCTGGATGAT GGAGATTCTA AGATCCAGAA CCAGCTCAAG
    AAACTGCAGA GCCCCATCAT TCTTCTCTAT TGTACTAAGG AAGAAGCCAC GTATATCTTT
    GAAGTAGCTA ACTCAGTAGG GCTGACTGGC TACGGCTACA CATGGATTGT ACCTAGTCTG
    GTGGCAGGGG ATACAGACAC AGTGCCCTCG GAGTTCCCCA CTGGCCTCAT TTCTGTGTCC
    TATGATGAAT GGGACTATGG CCTCCCTGCC AGAGTGAGGG ATGGAATTGC CATAATCACC
    ACTGCTGCTT CCGACATGCT GTCTGAACAC AGCTTCATCC CTGAGCCCAA GAGCAGTTGT
    TACAACACCC ATGAGAAGAG AATCTACCAG TCCAACATGC TAAATAGATG CAGAGACAGT
    AAAGATAACA AGATTTGGAG AAAAAAGAAT AACCAAAAGA AGGGACCATT TCACAGGGCT
    GTTGATTTTG TGTATCTGAT CAATGTCACT TTTGAGGGAA GAAATCTGTC CTTCAGTGAA
    GATGGCTACC AGATGCACCC GAAACTGGTG ATAATCCTTC TGAACAAGGA GAGAAAGTGG
    GAGAGGGTGG GGAAGTGGAA AGACAAATCC TTGCAGATGA AGTACTACGT GTGGCCCCGA
    ATGTGTCCTG AGACAGAAGA ACAGGAGGAT GACCATCTGA GCATTGTAAC CCTGGAGGAG
    GCCCCCTTTG TCATTGTGGA GAGTGTGGAC CCTCTGAGTG GAACCTGCAT GAGGAACACA
    GTCCCCTGCC AAAAACGCAT AGTCTCTGAG AATAAAACAG ATGAAGAGCC AGGTTACATC
    AAAAAGTGCT GCAAGGGGTT CTGTATTGAC ATCCTTAAGA AAATTTCTAA ATCTGTGAAG
    TTCACCTATG ATCTCTACCT GGTTACCAAT GGCAAGCATG GGAAGAAAAT CAATGGAACT
    TGGAATGGTA TGATTGGAGA GGTGGTTATG AAGAGGGCTT ACATGGCGGT GGGATCACTC
    ACCATCAATG AGGAGCGATC TGAGGTGGTT GACTTCTCTG TGCCCTTCAT AGAGACTGGC
    ATCAGTGTTA TGGTGTCACG CAGCAATGGG ACTGTCTCAC CATCTGCCTT CTTAGAGCCA
    TTCAGTGCTG ACGTATGGGT TATGATGTTC GTGATGCTGC TCATCGTCTC AGCCGTGGCT
    GTCTTTGTCT TCGAGTACTT CAGCCCTGTG GGTTACAACA GGTGCCTCGC TGATGGCAGA
    GAGCCAGGTG GCCCGTCTTT TACCATCGGC AAAGCTATTT GGTTACTGTG GGGTCTGGTG
    TTTAACAACT CCGTACCCGT GCAGAACCCA AAGGGGACCA CTTCCAAGAT CATGGTGTCG
    GTGTGGGCCT TCTTTGCCGT CATCTTCCTG GCCAGCTACA CTGCCAACTT AGCTGCCTTC
    ATGATCCAAG AAGAGTATGT GGACCAGGTT TCTGGCCTGA GTGACAAAAA GTTCCAGAGA
    CCTAATGACT TCTCACCCCC TTTTCGCTTT GGGACTGTGC CCAATGGCAG CACAGAGAGG
    AATATTCGCA ATAACTACGC AGAAATGCAT GCCTACATGG GAAAGTTCAA CCAGAGGGGT
    GTAGATGATG CATTGCTCTC CCTGAAAACA GGGAAACTGG ATGCCTTCAT CTATGATGCA
    GCAGTGCTGA ACTACATGGC GGGCAGAGAT GAAGGCTGCA AGCTGGTGAC CATTGGCAGT
    GGGAAAGTCT TTGCTTCCAC TGGTTATGGC ATTGCCATCC AAAAAGATTC TGGGTGGAAG
    CGCCAGGTGG ACCTTGCTAT CCTCCAGCTA TTCGGAGATG GGGAGATGGA GGAGCTAGAA
    GCTCTTTGGC TCACTGGCAT TTGCCACAAT GAAAAGAATG AGGTCATGAG CAGCCAGCTG
    GACATTGACA ACATGGCAGG AGTCTTCTAC ATGTTGGGGG CAGCCATGGC TCTCAGTCTC
    ATCACCTTCA TCTGTGAACA CCTTTTCTAT TGGCAGTTCC GGCACTGCTT TATGGGTGTC
    TGTTCCGGCA AGCCTGGCAT GGTCTTCTCC ATCAGCAGAG GTATCTACAG TTGCATCCAT
    GGAGTGGCTA TCGAGGAGCG CCAGTCCGTG ATGAATTCCC CCACCGCGAC CATGAACAAC
    ACACACTCCA ACATCCTGCG CCTGCTCCGC ACGGCCAAGA ACATGGCCAA CCTGTCTGGT
    GTGAACGGCT CCCCCCAGAG TGCCCTGGAC TTCATCCGAC GTGAGTCGTC CGTCTATGAC
    ATCTCCGAGC ACCGCCGCAG CTTCACGCAT TCCGACTGCA AATCCTACAA CAACCCGCCG
    TGCGAGGAGA ACCTCTTCAG CGACTACATC AGTGAGGTGG AGAGGACTTT TGGTAACCTG
    CAGCTGAAGG ACAGCAACGT GTACCAAGAT CACTATCACC ACCACCACCG GCCCCACAGC
    ATCGGCAGCG CCAGCTCCAT CGACGGGCTC TACGACTGTG ACAACCCGCC TTTCACCACC
    CAGCCGAGGT CCATCAGCAA GAAGCCCCTG GACCTCGGCC TCCCCTCCTC CAAACACAGC
    CAGCTCAGTG ACCTGTACGG CAAATTCTCC TTCAAGAGCG ACCGCTACAG TGGCCACGAT
    GACTTGATCC GCTCGGACGT GTCTGACATC TCCACGCACA CGGTCACCTA CGGAAACATA
    GAGGGCAACG CCGCCAAGAG GCGCAAGCAG CAATACAAGG ACAGCCTGAA GAAGCGGCCG
    GCCTCGGCCA AGTCCCGGCG GGAGTTCGAC GAGATCGAGC TGGCCTACCG CCGCCGGCCG
    CCCCGCTCCC CTGACCACAA GCGCTACTTC AGGGACAAGG AGGGGCTCCG GGACTTCTAC
    CTGGACCAGT TCCGCACCAA GGAGAACTCC CCGCACTGGG AGCACGTGGA CCTGACGGAC
    ATCTACAAGG AGCAGAGCGA TGACTTTAAG CGCGACTCGG TGAGCGGAGG AGGGCCCTGT
    ACCAACAGGT CTCACCTCAA ACACGGGGCG GGCGACAAGC ACGGCGTGGT CAGCGGGGTC
    CCGGCCCCTT GGGAGAAGAA CCTGAGCAAC GTGGATTGGG AGGACCGCTC CGGGGGCAAC
    TTTTGCCGCA GCTGCCCCTC AAAGCTGCAC AACTACTCGA CGGCGGTGAC CGGGCAGAAC
    TCGGGCCGGC AGGCGTGCAT CCGGTGCGAG GCCTGCAAGA AGGCAGGGAA CCTGTACGAC
    ATCAGCGAGG ACAACTCCCT GCAGGAGCTG GACCAGCCGG CTGCCCCTGT GGCGGTGCCG
    TCAAACGCGC CCAGCACCAA GTACCCCCAG AGCCCGACCA ACTCCAAGGC CCAGAAGAAG
    ACCCGGAACA AGCTGCGCCG GCAGCACTCC TACGACACCT TCGTGGACCT GCAGAAGGAA
    GAAGCCGCCT TGGCCCCGCG CAGCGTGAGC CTGAAAGACA AGGGCCGGTT CCTGGACGGG
    AGTCCCTACG CCCACATGTT TGAGACGCCA GCTGGCGAGA GCACCTTTGC CAACCACGAG
    TCCTCAGTGG CCGCCGCCGG ACACCGCCAC CACAACAACC CCGGCGGCGG CGGCTACATG
    CTCAGCAAGT CGCTCTACCC CGACCGGGTC ACGCAAAACC CTTTCATCCC CACTTTTGGG
    GACGACCAGT GCTTGCTCCA TGGCAGCAAA TCCTACTTCT TCAGGCAGCC CACGGTGGCG
    GGGGCGCCGA AAGCCAGGCC AGACTTCCGC GCCCTTGTCA CCAACAAGCC GGTGGTCTCG
    GCCCTGCACG GGGCCGTGCC AGGCCGTTTC CAGAAAGACA TCTGTATAGG GAACCAGTCC
    AACCCCTGTG TGCCTAACAA CAAAAACCCC AGGGCTTTCA ATGGCTCCAG CAATGGGCAT
    GTTTATGAGA AACTTTCTAG TATTGAGTCT GATGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26830
    Clone ID Related Accession (Same CDS sequence) XM_022410867.1 , XM_022410868.1
    Accession Version XM_022410868.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4119bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product glutamate receptor ionotropic, NMDA 2B isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTTCTTCC AGTTTGGCCC ATCAATTGAA CAGCAAGCTT CTGTAATGCT CAATATCATG 
    GAAGAGTATG ACTGGTACAT CTTTTCTATT GTCACCACCT ACTTCCCTGG CTACCAGGAC
    TTTGTAAACA AGATTCGCAG CACCATTGAG AACAGCTTCG TGGGCTGGGA GTTAGAGGAA
    GTCCTCCTGC TGGACATGTC CCTGGATGAT GGAGATTCTA AGATCCAGAA CCAGCTCAAG
    AAACTGCAGA GCCCCATCAT TCTTCTCTAT TGTACTAAGG AAGAAGCCAC GTATATCTTT
    GAAGTAGCTA ACTCAGTAGG GCTGACTGGC TACGGCTACA CATGGATTGT ACCTAGTCTG
    GTGGCAGGGG ATACAGACAC AGTGCCCTCG GAGTTCCCCA CTGGCCTCAT TTCTGTGTCC
    TATGATGAAT GGGACTATGG CCTCCCTGCC AGAGTGAGGG ATGGAATTGC CATAATCACC
    ACTGCTGCTT CCGACATGCT GTCTGAACAC AGCTTCATCC CTGAGCCCAA GAGCAGTTGT
    TACAACACCC ATGAGAAGAG AATCTACCAG TCCAACATGC TAAATAGATG CAGAGACAGT
    AAAGATAACA AGATTTGGAG AAAAAAGAAT AACCAAAAGA AGGGACCATT TCACAGGGCT
    GTTGATTTTG TGTATCTGAT CAATGTCACT TTTGAGGGAA GAAATCTGTC CTTCAGTGAA
    GATGGCTACC AGATGCACCC GAAACTGGTG ATAATCCTTC TGAACAAGGA GAGAAAGTGG
    GAGAGGGTGG GGAAGTGGAA AGACAAATCC TTGCAGATGA AGTACTACGT GTGGCCCCGA
    ATGTGTCCTG AGACAGAAGA ACAGGAGGAT GACCATCTGA GCATTGTAAC CCTGGAGGAG
    GCCCCCTTTG TCATTGTGGA GAGTGTGGAC CCTCTGAGTG GAACCTGCAT GAGGAACACA
    GTCCCCTGCC AAAAACGCAT AGTCTCTGAG AATAAAACAG ATGAAGAGCC AGGTTACATC
    AAAAAGTGCT GCAAGGGGTT CTGTATTGAC ATCCTTAAGA AAATTTCTAA ATCTGTGAAG
    TTCACCTATG ATCTCTACCT GGTTACCAAT GGCAAGCATG GGAAGAAAAT CAATGGAACT
    TGGAATGGTA TGATTGGAGA GGTGGTTATG AAGAGGGCTT ACATGGCGGT GGGATCACTC
    ACCATCAATG AGGAGCGATC TGAGGTGGTT GACTTCTCTG TGCCCTTCAT AGAGACTGGC
    ATCAGTGTTA TGGTGTCACG CAGCAATGGG ACTGTCTCAC CATCTGCCTT CTTAGAGCCA
    TTCAGTGCTG ACGTATGGGT TATGATGTTC GTGATGCTGC TCATCGTCTC AGCCGTGGCT
    GTCTTTGTCT TCGAGTACTT CAGCCCTGTG GGTTACAACA GGTGCCTCGC TGATGGCAGA
    GAGCCAGGTG GCCCGTCTTT TACCATCGGC AAAGCTATTT GGTTACTGTG GGGTCTGGTG
    TTTAACAACT CCGTACCCGT GCAGAACCCA AAGGGGACCA CTTCCAAGAT CATGGTGTCG
    GTGTGGGCCT TCTTTGCCGT CATCTTCCTG GCCAGCTACA CTGCCAACTT AGCTGCCTTC
    ATGATCCAAG AAGAGTATGT GGACCAGGTT TCTGGCCTGA GTGACAAAAA GTTCCAGAGA
    CCTAATGACT TCTCACCCCC TTTTCGCTTT GGGACTGTGC CCAATGGCAG CACAGAGAGG
    AATATTCGCA ATAACTACGC AGAAATGCAT GCCTACATGG GAAAGTTCAA CCAGAGGGGT
    GTAGATGATG CATTGCTCTC CCTGAAAACA GGGAAACTGG ATGCCTTCAT CTATGATGCA
    GCAGTGCTGA ACTACATGGC GGGCAGAGAT GAAGGCTGCA AGCTGGTGAC CATTGGCAGT
    GGGAAAGTCT TTGCTTCCAC TGGTTATGGC ATTGCCATCC AAAAAGATTC TGGGTGGAAG
    CGCCAGGTGG ACCTTGCTAT CCTCCAGCTA TTCGGAGATG GGGAGATGGA GGAGCTAGAA
    GCTCTTTGGC TCACTGGCAT TTGCCACAAT GAAAAGAATG AGGTCATGAG CAGCCAGCTG
    GACATTGACA ACATGGCAGG AGTCTTCTAC ATGTTGGGGG CAGCCATGGC TCTCAGTCTC
    ATCACCTTCA TCTGTGAACA CCTTTTCTAT TGGCAGTTCC GGCACTGCTT TATGGGTGTC
    TGTTCCGGCA AGCCTGGCAT GGTCTTCTCC ATCAGCAGAG GTATCTACAG TTGCATCCAT
    GGAGTGGCTA TCGAGGAGCG CCAGTCCGTG ATGAATTCCC CCACCGCGAC CATGAACAAC
    ACACACTCCA ACATCCTGCG CCTGCTCCGC ACGGCCAAGA ACATGGCCAA CCTGTCTGGT
    GTGAACGGCT CCCCCCAGAG TGCCCTGGAC TTCATCCGAC GTGAGTCGTC CGTCTATGAC
    ATCTCCGAGC ACCGCCGCAG CTTCACGCAT TCCGACTGCA AATCCTACAA CAACCCGCCG
    TGCGAGGAGA ACCTCTTCAG CGACTACATC AGTGAGGTGG AGAGGACTTT TGGTAACCTG
    CAGCTGAAGG ACAGCAACGT GTACCAAGAT CACTATCACC ACCACCACCG GCCCCACAGC
    ATCGGCAGCG CCAGCTCCAT CGACGGGCTC TACGACTGTG ACAACCCGCC TTTCACCACC
    CAGCCGAGGT CCATCAGCAA GAAGCCCCTG GACCTCGGCC TCCCCTCCTC CAAACACAGC
    CAGCTCAGTG ACCTGTACGG CAAATTCTCC TTCAAGAGCG ACCGCTACAG TGGCCACGAT
    GACTTGATCC GCTCGGACGT GTCTGACATC TCCACGCACA CGGTCACCTA CGGAAACATA
    GAGGGCAACG CCGCCAAGAG GCGCAAGCAG CAATACAAGG ACAGCCTGAA GAAGCGGCCG
    GCCTCGGCCA AGTCCCGGCG GGAGTTCGAC GAGATCGAGC TGGCCTACCG CCGCCGGCCG
    CCCCGCTCCC CTGACCACAA GCGCTACTTC AGGGACAAGG AGGGGCTCCG GGACTTCTAC
    CTGGACCAGT TCCGCACCAA GGAGAACTCC CCGCACTGGG AGCACGTGGA CCTGACGGAC
    ATCTACAAGG AGCAGAGCGA TGACTTTAAG CGCGACTCGG TGAGCGGAGG AGGGCCCTGT
    ACCAACAGGT CTCACCTCAA ACACGGGGCG GGCGACAAGC ACGGCGTGGT CAGCGGGGTC
    CCGGCCCCTT GGGAGAAGAA CCTGAGCAAC GTGGATTGGG AGGACCGCTC CGGGGGCAAC
    TTTTGCCGCA GCTGCCCCTC AAAGCTGCAC AACTACTCGA CGGCGGTGAC CGGGCAGAAC
    TCGGGCCGGC AGGCGTGCAT CCGGTGCGAG GCCTGCAAGA AGGCAGGGAA CCTGTACGAC
    ATCAGCGAGG ACAACTCCCT GCAGGAGCTG GACCAGCCGG CTGCCCCTGT GGCGGTGCCG
    TCAAACGCGC CCAGCACCAA GTACCCCCAG AGCCCGACCA ACTCCAAGGC CCAGAAGAAG
    ACCCGGAACA AGCTGCGCCG GCAGCACTCC TACGACACCT TCGTGGACCT GCAGAAGGAA
    GAAGCCGCCT TGGCCCCGCG CAGCGTGAGC CTGAAAGACA AGGGCCGGTT CCTGGACGGG
    AGTCCCTACG CCCACATGTT TGAGACGCCA GCTGGCGAGA GCACCTTTGC CAACCACGAG
    TCCTCAGTGG CCGCCGCCGG ACACCGCCAC CACAACAACC CCGGCGGCGG CGGCTACATG
    CTCAGCAAGT CGCTCTACCC CGACCGGGTC ACGCAAAACC CTTTCATCCC CACTTTTGGG
    GACGACCAGT GCTTGCTCCA TGGCAGCAAA TCCTACTTCT TCAGGCAGCC CACGGTGGCG
    GGGGCGCCGA AAGCCAGGCC AGACTTCCGC GCCCTTGTCA CCAACAAGCC GGTGGTCTCG
    GCCCTGCACG GGGCCGTGCC AGGCCGTTTC CAGAAAGACA TCTGTATAGG GAACCAGTCC
    AACCCCTGTG TGCCTAACAA CAAAAACCCC AGGGCTTTCA ATGGCTCCAG CAATGGGCAT
    GTTTATGAGA AACTTTCTAG TATTGAGTCT GATGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022266576.1
    CDS120..4238
    Translation

    Target ORF information:

    RefSeq Version XM_022410868.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris glutamate ionotropic receptor NMDA type subunit 2B (GRIN2B), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022410868.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTTCTTCC AGTTTGGCCC ATCAATTGAA CAGCAAGCTT CTGTAATGCT CAATATCATG 
    GAAGAGTATG ACTGGTACAT CTTTTCTATT GTCACCACCT ACTTCCCTGG CTACCAGGAC
    TTTGTAAACA AGATTCGCAG CACCATTGAG AACAGCTTCG TGGGCTGGGA GTTAGAGGAA
    GTCCTCCTGC TGGACATGTC CCTGGATGAT GGAGATTCTA AGATCCAGAA CCAGCTCAAG
    AAACTGCAGA GCCCCATCAT TCTTCTCTAT TGTACTAAGG AAGAAGCCAC GTATATCTTT
    GAAGTAGCTA ACTCAGTAGG GCTGACTGGC TACGGCTACA CATGGATTGT ACCTAGTCTG
    GTGGCAGGGG ATACAGACAC AGTGCCCTCG GAGTTCCCCA CTGGCCTCAT TTCTGTGTCC
    TATGATGAAT GGGACTATGG CCTCCCTGCC AGAGTGAGGG ATGGAATTGC CATAATCACC
    ACTGCTGCTT CCGACATGCT GTCTGAACAC AGCTTCATCC CTGAGCCCAA GAGCAGTTGT
    TACAACACCC ATGAGAAGAG AATCTACCAG TCCAACATGC TAAATAGATG CAGAGACAGT
    AAAGATAACA AGATTTGGAG AAAAAAGAAT AACCAAAAGA AGGGACCATT TCACAGGGCT
    GTTGATTTTG TGTATCTGAT CAATGTCACT TTTGAGGGAA GAAATCTGTC CTTCAGTGAA
    GATGGCTACC AGATGCACCC GAAACTGGTG ATAATCCTTC TGAACAAGGA GAGAAAGTGG
    GAGAGGGTGG GGAAGTGGAA AGACAAATCC TTGCAGATGA AGTACTACGT GTGGCCCCGA
    ATGTGTCCTG AGACAGAAGA ACAGGAGGAT GACCATCTGA GCATTGTAAC CCTGGAGGAG
    GCCCCCTTTG TCATTGTGGA GAGTGTGGAC CCTCTGAGTG GAACCTGCAT GAGGAACACA
    GTCCCCTGCC AAAAACGCAT AGTCTCTGAG AATAAAACAG ATGAAGAGCC AGGTTACATC
    AAAAAGTGCT GCAAGGGGTT CTGTATTGAC ATCCTTAAGA AAATTTCTAA ATCTGTGAAG
    TTCACCTATG ATCTCTACCT GGTTACCAAT GGCAAGCATG GGAAGAAAAT CAATGGAACT
    TGGAATGGTA TGATTGGAGA GGTGGTTATG AAGAGGGCTT ACATGGCGGT GGGATCACTC
    ACCATCAATG AGGAGCGATC TGAGGTGGTT GACTTCTCTG TGCCCTTCAT AGAGACTGGC
    ATCAGTGTTA TGGTGTCACG CAGCAATGGG ACTGTCTCAC CATCTGCCTT CTTAGAGCCA
    TTCAGTGCTG ACGTATGGGT TATGATGTTC GTGATGCTGC TCATCGTCTC AGCCGTGGCT
    GTCTTTGTCT TCGAGTACTT CAGCCCTGTG GGTTACAACA GGTGCCTCGC TGATGGCAGA
    GAGCCAGGTG GCCCGTCTTT TACCATCGGC AAAGCTATTT GGTTACTGTG GGGTCTGGTG
    TTTAACAACT CCGTACCCGT GCAGAACCCA AAGGGGACCA CTTCCAAGAT CATGGTGTCG
    GTGTGGGCCT TCTTTGCCGT CATCTTCCTG GCCAGCTACA CTGCCAACTT AGCTGCCTTC
    ATGATCCAAG AAGAGTATGT GGACCAGGTT TCTGGCCTGA GTGACAAAAA GTTCCAGAGA
    CCTAATGACT TCTCACCCCC TTTTCGCTTT GGGACTGTGC CCAATGGCAG CACAGAGAGG
    AATATTCGCA ATAACTACGC AGAAATGCAT GCCTACATGG GAAAGTTCAA CCAGAGGGGT
    GTAGATGATG CATTGCTCTC CCTGAAAACA GGGAAACTGG ATGCCTTCAT CTATGATGCA
    GCAGTGCTGA ACTACATGGC GGGCAGAGAT GAAGGCTGCA AGCTGGTGAC CATTGGCAGT
    GGGAAAGTCT TTGCTTCCAC TGGTTATGGC ATTGCCATCC AAAAAGATTC TGGGTGGAAG
    CGCCAGGTGG ACCTTGCTAT CCTCCAGCTA TTCGGAGATG GGGAGATGGA GGAGCTAGAA
    GCTCTTTGGC TCACTGGCAT TTGCCACAAT GAAAAGAATG AGGTCATGAG CAGCCAGCTG
    GACATTGACA ACATGGCAGG AGTCTTCTAC ATGTTGGGGG CAGCCATGGC TCTCAGTCTC
    ATCACCTTCA TCTGTGAACA CCTTTTCTAT TGGCAGTTCC GGCACTGCTT TATGGGTGTC
    TGTTCCGGCA AGCCTGGCAT GGTCTTCTCC ATCAGCAGAG GTATCTACAG TTGCATCCAT
    GGAGTGGCTA TCGAGGAGCG CCAGTCCGTG ATGAATTCCC CCACCGCGAC CATGAACAAC
    ACACACTCCA ACATCCTGCG CCTGCTCCGC ACGGCCAAGA ACATGGCCAA CCTGTCTGGT
    GTGAACGGCT CCCCCCAGAG TGCCCTGGAC TTCATCCGAC GTGAGTCGTC CGTCTATGAC
    ATCTCCGAGC ACCGCCGCAG CTTCACGCAT TCCGACTGCA AATCCTACAA CAACCCGCCG
    TGCGAGGAGA ACCTCTTCAG CGACTACATC AGTGAGGTGG AGAGGACTTT TGGTAACCTG
    CAGCTGAAGG ACAGCAACGT GTACCAAGAT CACTATCACC ACCACCACCG GCCCCACAGC
    ATCGGCAGCG CCAGCTCCAT CGACGGGCTC TACGACTGTG ACAACCCGCC TTTCACCACC
    CAGCCGAGGT CCATCAGCAA GAAGCCCCTG GACCTCGGCC TCCCCTCCTC CAAACACAGC
    CAGCTCAGTG ACCTGTACGG CAAATTCTCC TTCAAGAGCG ACCGCTACAG TGGCCACGAT
    GACTTGATCC GCTCGGACGT GTCTGACATC TCCACGCACA CGGTCACCTA CGGAAACATA
    GAGGGCAACG CCGCCAAGAG GCGCAAGCAG CAATACAAGG ACAGCCTGAA GAAGCGGCCG
    GCCTCGGCCA AGTCCCGGCG GGAGTTCGAC GAGATCGAGC TGGCCTACCG CCGCCGGCCG
    CCCCGCTCCC CTGACCACAA GCGCTACTTC AGGGACAAGG AGGGGCTCCG GGACTTCTAC
    CTGGACCAGT TCCGCACCAA GGAGAACTCC CCGCACTGGG AGCACGTGGA CCTGACGGAC
    ATCTACAAGG AGCAGAGCGA TGACTTTAAG CGCGACTCGG TGAGCGGAGG AGGGCCCTGT
    ACCAACAGGT CTCACCTCAA ACACGGGGCG GGCGACAAGC ACGGCGTGGT CAGCGGGGTC
    CCGGCCCCTT GGGAGAAGAA CCTGAGCAAC GTGGATTGGG AGGACCGCTC CGGGGGCAAC
    TTTTGCCGCA GCTGCCCCTC AAAGCTGCAC AACTACTCGA CGGCGGTGAC CGGGCAGAAC
    TCGGGCCGGC AGGCGTGCAT CCGGTGCGAG GCCTGCAAGA AGGCAGGGAA CCTGTACGAC
    ATCAGCGAGG ACAACTCCCT GCAGGAGCTG GACCAGCCGG CTGCCCCTGT GGCGGTGCCG
    TCAAACGCGC CCAGCACCAA GTACCCCCAG AGCCCGACCA ACTCCAAGGC CCAGAAGAAG
    ACCCGGAACA AGCTGCGCCG GCAGCACTCC TACGACACCT TCGTGGACCT GCAGAAGGAA
    GAAGCCGCCT TGGCCCCGCG CAGCGTGAGC CTGAAAGACA AGGGCCGGTT CCTGGACGGG
    AGTCCCTACG CCCACATGTT TGAGACGCCA GCTGGCGAGA GCACCTTTGC CAACCACGAG
    TCCTCAGTGG CCGCCGCCGG ACACCGCCAC CACAACAACC CCGGCGGCGG CGGCTACATG
    CTCAGCAAGT CGCTCTACCC CGACCGGGTC ACGCAAAACC CTTTCATCCC CACTTTTGGG
    GACGACCAGT GCTTGCTCCA TGGCAGCAAA TCCTACTTCT TCAGGCAGCC CACGGTGGCG
    GGGGCGCCGA AAGCCAGGCC AGACTTCCGC GCCCTTGTCA CCAACAAGCC GGTGGTCTCG
    GCCCTGCACG GGGCCGTGCC AGGCCGTTTC CAGAAAGACA TCTGTATAGG GAACCAGTCC
    AACCCCTGTG TGCCTAACAA CAAAAACCCC AGGGCTTTCA ATGGCTCCAG CAATGGGCAT
    GTTTATGAGA AACTTTCTAG TATTGAGTCT GATGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe43518
    Clone ID Related Accession (Same CDS sequence) XM_014108269.1 , XM_014108270.1
    Accession Version XM_014108269.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4035bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product glutamate receptor ionotropic, NMDA 2B isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTTCTTCC AGTTTGGCCC ATCAATTGAA CAGCAAGCTT CTGTAATGCT CAATATCATG 
    GAAGAGTATG ACTGGTACAT CTTTTCTATT GTCACCACCT ACTTCCCTGG CTACCAGGAC
    TTTGTAAACA AGATTCGCAG CACCATTGAG AACAGCTTCG TGGGCTGGGA GTTAGAGGAA
    GTCCTCCTGC TGGACATGTC CCTGGATGAT GGAGATTCTA AGATCCAGAA CCAGCTCAAG
    AAACTGCAGA GCCCCATCAT TCTTCTCTAT TGTACTAAGG AAGAAGCCAC GTATATCTTT
    GAAGTAGCTA ACTCAGTAGG GCTGACTGGC TACGGCTACA CATGGATTGT ACCTAGTCTG
    GTGGCAGGGG ATACAGACAC AGTGCCCTCG GAGTTCCCCA CTGGCCTCAT TTCTGTGTCC
    TATGATGAAT GGGACTATGG CCTCCCTGCC AGAGTGAGGG ATGGAATTGC CATAATCACC
    ACTGCTGCTT CCGACATGCT GTCTGAACAC AGCTTCATCC CTGAGCCCAA GAGCAGTTGT
    TACAACACCC ATGAGAAGAG AATCTACCAG TCCAACATGC TAAATAGGTA TCTGATCAAT
    GTCACTTTTG AGGGAAGAAA TCTGTCCTTC AGTGAAGATG GCTACCAGAT GCACCCGAAA
    CTGGTGATAA TCCTTCTGAA CAAGGAGAGA AAGTGGGAGA GGGTGGGGAA GTGGAAAGAC
    AAATCCTTGC AGATGAAGTA CTACGTGTGG CCCCGAATGT GTCCTGAGAC AGAAGAACAG
    GAGGATGACC ATCTGAGCAT TGTAACCCTG GAGGAGGCCC CCTTTGTCAT TGTGGAGAGT
    GTGGACCCTC TGAGTGGAAC CTGCATGAGG AACACAGTCC CCTGCCAAAA ACGCATAGTC
    TCTGAGAATA AAACAGATGA AGAGCCAGGT TACATCAAAA AGTGCTGCAA GGGGTTCTGT
    ATTGACATCC TTAAGAAAAT TTCTAAATCT GTGAAGTTCA CCTATGATCT CTACCTGGTT
    ACCAATGGCA AGCATGGGAA GAAAATCAAT GGAACTTGGA ATGGTATGAT TGGAGAGGTG
    GTTATGAAGA GGGCTTACAT GGCGGTGGGA TCACTCACCA TCAATGAGGA GCGATCTGAG
    GTGGTTGACT TCTCTGTGCC CTTCATAGAG ACTGGCATCA GTGTTATGGT GTCACGCAGC
    AATGGGACTG TCTCACCATC TGCCTTCTTA GAGCCATTCA GTGCTGACGT ATGGGTTATG
    ATGTTCGTGA TGCTGCTCAT CGTCTCAGCC GTGGCTGTCT TTGTCTTCGA GTACTTCAGC
    CCTGTGGGTT ACAACAGGTG CCTCGCTGAT GGCAGAGAGC CAGGTGGCCC GTCTTTTACC
    ATCGGCAAAG CTATTTGGTT ACTGTGGGGT CTGGTGTTTA ACAACTCCGT ACCCGTGCAG
    AACCCAAAGG GGACCACTTC CAAGATCATG GTGTCGGTGT GGGCCTTCTT TGCCGTCATC
    TTCCTGGCCA GCTACACTGC CAACTTAGCT GCCTTCATGA TCCAAGAAGA GTATGTGGAC
    CAGGTTTCTG GCCTGAGTGA CAAAAAGTTC CAGAGACCTA ATGACTTCTC ACCCCCTTTT
    CGCTTTGGGA CTGTGCCCAA TGGCAGCACA GAGAGGAATA TTCGCAATAA CTACGCAGAA
    ATGCATGCCT ACATGGGAAA GTTCAACCAG AGGGGTGTAG ATGATGCATT GCTCTCCCTG
    AAAACAGGGA AACTGGATGC CTTCATCTAT GATGCAGCAG TGCTGAACTA CATGGCGGGC
    AGAGATGAAG GCTGCAAGCT GGTGACCATT GGCAGTGGGA AAGTCTTTGC TTCCACTGGT
    TATGGCATTG CCATCCAAAA AGATTCTGGG TGGAAGCGCC AGGTGGACCT TGCTATCCTC
    CAGCTATTCG GAGATGGGGA GATGGAGGAG CTAGAAGCTC TTTGGCTCAC TGGCATTTGC
    CACAATGAAA AGAATGAGGT CATGAGCAGC CAGCTGGACA TTGACAACAT GGCAGGAGTC
    TTCTACATGT TGGGGGCAGC CATGGCTCTC AGTCTCATCA CCTTCATCTG TGAACACCTT
    TTCTATTGGC AGTTCCGGCA CTGCTTTATG GGTGTCTGTT CCGGCAAGCC TGGCATGGTC
    TTCTCCATCA GCAGAGGTAT CTACAGTTGC ATCCATGGAG TGGCTATCGA GGAGCGCCAG
    TCCGTGATGA ATTCCCCCAC CGCGACCATG AACAACACAC ACTCCAACAT CCTGCGCCTG
    CTCCGCACGG CCAAGAACAT GGCCAACCTG TCTGGTGTGA ACGGCTCCCC CCAGAGTGCC
    CTGGACTTCA TCCGACGTGA GTCGTCCGTC TATGACATCT CCGAGCACCG CCGCAGCTTC
    ACGCATTCCG ACTGCAAATC CTACAACAAC CCGCCGTGCG AGGAGAACCT CTTCAGCGAC
    TACATCAGTG AGGTGGAGAG GACTTTTGGT AACCTGCAGC TGAAGGACAG CAACGTGTAC
    CAAGATCACT ATCACCACCA CCACCGGCCC CACAGCATCG GCAGCGCCAG CTCCATCGAC
    GGGCTCTACG ACTGTGACAA CCCGCCTTTC ACCACCCAGC CGAGGTCCAT CAGCAAGAAG
    CCCCTGGACC TCGGCCTCCC CTCCTCCAAA CACAGCCAGC TCAGTGACCT GTACGGCAAA
    TTCTCCTTCA AGAGCGACCG CTACAGTGGC CACGATGACT TGATCCGCTC GGACGTGTCT
    GACATCTCCA CGCACACGGT CACCTACGGA AACATAGAGG GCAACGCCGC CAAGAGGCGC
    AAGCAGCAAT ACAAGGACAG CCTGAAGAAG CGGCCGGCCT CGGCCAAGTC CCGGCGGGAG
    TTCGACGAGA TCGAGCTGGC CTACCGCCGC CGGCCGCCCC GCTCCCCTGA CCACAAGCGC
    TACTTCAGGG ACAAGGAGGG GCTCCGGGAC TTCTACCTGG ACCAGTTCCG CACCAAGGAG
    AACTCCCCGC ACTGGGAGCA CGTGGACCTG ACGGACATCT ACAAGGAGCA GAGCGATGAC
    TTTAAGCGCG ACTCGGTGAG CGGAGGAGGG CCCTGTACCA ACAGGTCTCA CCTCAAACAC
    GGGGCGGGCG ACAAGCACGG CGTGGTCAGC GGGGTCCCGG CCCCTTGGGA GAAGAACCTG
    AGCAACGTGG ATTGGGAGGA CCGCTCCGGG GGCAACTTTT GCCGCAGCTG CCCCTCAAAG
    CTGCACAACT ACTCGACGGC GGTGACCGGG CAGAACTCGG GCCGGCAGGC GTGCATCCGG
    TGCGAGGCCT GCAAGAAGGC AGGGAACCTG TACGACATCA GCGAGGACAA CTCCCTGCAG
    GAGCTGGACC AGCCGGCTGC CCCTGTGGCG GTGCCGTCAA ACGCGCCCAG CACCAAGTAC
    CCCCAGAGCC CGACCAACTC CAAGGCCCAG AAGAAGACCC GGAACAAGCT GCGCCGGCAG
    CACTCCTACG ACACCTTCGT GGACCTGCAG AAGGAAGAAG CCGCCTTGGC CCCGCGCAGC
    GTGAGCCTGA AAGACAAGGG CCGGTTCCTG GACGGGAGTC CCTACGCCCA CATGTTTGAG
    ACGCCAGCTG GCGAGAGCAC CTTTGCCAAC CACGAGTCCT CAGTGGCCGC CGCCGGACAC
    CGCCACCACA ACAACCCCGG CGGCGGCGGC TACATGCTCA GCAAGTCGCT CTACCCCGAC
    CGGGTCACGC AAAACCCTTT CATCCCCACT TTTGGGGACG ACCAGTGCTT GCTCCATGGC
    AGCAAATCCT ACTTCTTCAG GCAGCCCACG GTGGCGGGGG CGCCGAAAGC CAGGCCAGAC
    TTCCGCGCCC TTGTCACCAA CAAGCCGGTG GTCTCGGCCC TGCACGGGGC CGTGCCAGGC
    CGTTTCCAGA AAGACATCTG TATAGGGAAC CAGTCCAACC CCTGTGTGCC TAACAACAAA
    AACCCCAGGG CTTTCAATGG CTCCAGCAAT GGGCATGTTT ATGAGAAACT TTCTAGTATT
    GAGTCTGATG TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963744.1
    CDS112..4146
    Translation

    Target ORF information:

    RefSeq Version XM_014108269.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris glutamate receptor, ionotropic, N-methyl D-aspartate 2B (GRIN2B), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014108269.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTTCTTCC AGTTTGGCCC ATCAATTGAA CAGCAAGCTT CTGTAATGCT CAATATCATG 
    GAAGAGTATG ACTGGTACAT CTTTTCTATT GTCACCACCT ACTTCCCTGG CTACCAGGAC
    TTTGTAAACA AGATTCGCAG CACCATTGAG AACAGCTTCG TGGGCTGGGA GTTAGAGGAA
    GTCCTCCTGC TGGACATGTC CCTGGATGAT GGAGATTCTA AGATCCAGAA CCAGCTCAAG
    AAACTGCAGA GCCCCATCAT TCTTCTCTAT TGTACTAAGG AAGAAGCCAC GTATATCTTT
    GAAGTAGCTA ACTCAGTAGG GCTGACTGGC TACGGCTACA CATGGATTGT ACCTAGTCTG
    GTGGCAGGGG ATACAGACAC AGTGCCCTCG GAGTTCCCCA CTGGCCTCAT TTCTGTGTCC
    TATGATGAAT GGGACTATGG CCTCCCTGCC AGAGTGAGGG ATGGAATTGC CATAATCACC
    ACTGCTGCTT CCGACATGCT GTCTGAACAC AGCTTCATCC CTGAGCCCAA GAGCAGTTGT
    TACAACACCC ATGAGAAGAG AATCTACCAG TCCAACATGC TAAATAGGTA TCTGATCAAT
    GTCACTTTTG AGGGAAGAAA TCTGTCCTTC AGTGAAGATG GCTACCAGAT GCACCCGAAA
    CTGGTGATAA TCCTTCTGAA CAAGGAGAGA AAGTGGGAGA GGGTGGGGAA GTGGAAAGAC
    AAATCCTTGC AGATGAAGTA CTACGTGTGG CCCCGAATGT GTCCTGAGAC AGAAGAACAG
    GAGGATGACC ATCTGAGCAT TGTAACCCTG GAGGAGGCCC CCTTTGTCAT TGTGGAGAGT
    GTGGACCCTC TGAGTGGAAC CTGCATGAGG AACACAGTCC CCTGCCAAAA ACGCATAGTC
    TCTGAGAATA AAACAGATGA AGAGCCAGGT TACATCAAAA AGTGCTGCAA GGGGTTCTGT
    ATTGACATCC TTAAGAAAAT TTCTAAATCT GTGAAGTTCA CCTATGATCT CTACCTGGTT
    ACCAATGGCA AGCATGGGAA GAAAATCAAT GGAACTTGGA ATGGTATGAT TGGAGAGGTG
    GTTATGAAGA GGGCTTACAT GGCGGTGGGA TCACTCACCA TCAATGAGGA GCGATCTGAG
    GTGGTTGACT TCTCTGTGCC CTTCATAGAG ACTGGCATCA GTGTTATGGT GTCACGCAGC
    AATGGGACTG TCTCACCATC TGCCTTCTTA GAGCCATTCA GTGCTGACGT ATGGGTTATG
    ATGTTCGTGA TGCTGCTCAT CGTCTCAGCC GTGGCTGTCT TTGTCTTCGA GTACTTCAGC
    CCTGTGGGTT ACAACAGGTG CCTCGCTGAT GGCAGAGAGC CAGGTGGCCC GTCTTTTACC
    ATCGGCAAAG CTATTTGGTT ACTGTGGGGT CTGGTGTTTA ACAACTCCGT ACCCGTGCAG
    AACCCAAAGG GGACCACTTC CAAGATCATG GTGTCGGTGT GGGCCTTCTT TGCCGTCATC
    TTCCTGGCCA GCTACACTGC CAACTTAGCT GCCTTCATGA TCCAAGAAGA GTATGTGGAC
    CAGGTTTCTG GCCTGAGTGA CAAAAAGTTC CAGAGACCTA ATGACTTCTC ACCCCCTTTT
    CGCTTTGGGA CTGTGCCCAA TGGCAGCACA GAGAGGAATA TTCGCAATAA CTACGCAGAA
    ATGCATGCCT ACATGGGAAA GTTCAACCAG AGGGGTGTAG ATGATGCATT GCTCTCCCTG
    AAAACAGGGA AACTGGATGC CTTCATCTAT GATGCAGCAG TGCTGAACTA CATGGCGGGC
    AGAGATGAAG GCTGCAAGCT GGTGACCATT GGCAGTGGGA AAGTCTTTGC TTCCACTGGT
    TATGGCATTG CCATCCAAAA AGATTCTGGG TGGAAGCGCC AGGTGGACCT TGCTATCCTC
    CAGCTATTCG GAGATGGGGA GATGGAGGAG CTAGAAGCTC TTTGGCTCAC TGGCATTTGC
    CACAATGAAA AGAATGAGGT CATGAGCAGC CAGCTGGACA TTGACAACAT GGCAGGAGTC
    TTCTACATGT TGGGGGCAGC CATGGCTCTC AGTCTCATCA CCTTCATCTG TGAACACCTT
    TTCTATTGGC AGTTCCGGCA CTGCTTTATG GGTGTCTGTT CCGGCAAGCC TGGCATGGTC
    TTCTCCATCA GCAGAGGTAT CTACAGTTGC ATCCATGGAG TGGCTATCGA GGAGCGCCAG
    TCCGTGATGA ATTCCCCCAC CGCGACCATG AACAACACAC ACTCCAACAT CCTGCGCCTG
    CTCCGCACGG CCAAGAACAT GGCCAACCTG TCTGGTGTGA ACGGCTCCCC CCAGAGTGCC
    CTGGACTTCA TCCGACGTGA GTCGTCCGTC TATGACATCT CCGAGCACCG CCGCAGCTTC
    ACGCATTCCG ACTGCAAATC CTACAACAAC CCGCCGTGCG AGGAGAACCT CTTCAGCGAC
    TACATCAGTG AGGTGGAGAG GACTTTTGGT AACCTGCAGC TGAAGGACAG CAACGTGTAC
    CAAGATCACT ATCACCACCA CCACCGGCCC CACAGCATCG GCAGCGCCAG CTCCATCGAC
    GGGCTCTACG ACTGTGACAA CCCGCCTTTC ACCACCCAGC CGAGGTCCAT CAGCAAGAAG
    CCCCTGGACC TCGGCCTCCC CTCCTCCAAA CACAGCCAGC TCAGTGACCT GTACGGCAAA
    TTCTCCTTCA AGAGCGACCG CTACAGTGGC CACGATGACT TGATCCGCTC GGACGTGTCT
    GACATCTCCA CGCACACGGT CACCTACGGA AACATAGAGG GCAACGCCGC CAAGAGGCGC
    AAGCAGCAAT ACAAGGACAG CCTGAAGAAG CGGCCGGCCT CGGCCAAGTC CCGGCGGGAG
    TTCGACGAGA TCGAGCTGGC CTACCGCCGC CGGCCGCCCC GCTCCCCTGA CCACAAGCGC
    TACTTCAGGG ACAAGGAGGG GCTCCGGGAC TTCTACCTGG ACCAGTTCCG CACCAAGGAG
    AACTCCCCGC ACTGGGAGCA CGTGGACCTG ACGGACATCT ACAAGGAGCA GAGCGATGAC
    TTTAAGCGCG ACTCGGTGAG CGGAGGAGGG CCCTGTACCA ACAGGTCTCA CCTCAAACAC
    GGGGCGGGCG ACAAGCACGG CGTGGTCAGC GGGGTCCCGG CCCCTTGGGA GAAGAACCTG
    AGCAACGTGG ATTGGGAGGA CCGCTCCGGG GGCAACTTTT GCCGCAGCTG CCCCTCAAAG
    CTGCACAACT ACTCGACGGC GGTGACCGGG CAGAACTCGG GCCGGCAGGC GTGCATCCGG
    TGCGAGGCCT GCAAGAAGGC AGGGAACCTG TACGACATCA GCGAGGACAA CTCCCTGCAG
    GAGCTGGACC AGCCGGCTGC CCCTGTGGCG GTGCCGTCAA ACGCGCCCAG CACCAAGTAC
    CCCCAGAGCC CGACCAACTC CAAGGCCCAG AAGAAGACCC GGAACAAGCT GCGCCGGCAG
    CACTCCTACG ACACCTTCGT GGACCTGCAG AAGGAAGAAG CCGCCTTGGC CCCGCGCAGC
    GTGAGCCTGA AAGACAAGGG CCGGTTCCTG GACGGGAGTC CCTACGCCCA CATGTTTGAG
    ACGCCAGCTG GCGAGAGCAC CTTTGCCAAC CACGAGTCCT CAGTGGCCGC CGCCGGACAC
    CGCCACCACA ACAACCCCGG CGGCGGCGGC TACATGCTCA GCAAGTCGCT CTACCCCGAC
    CGGGTCACGC AAAACCCTTT CATCCCCACT TTTGGGGACG ACCAGTGCTT GCTCCATGGC
    AGCAAATCCT ACTTCTTCAG GCAGCCCACG GTGGCGGGGG CGCCGAAAGC CAGGCCAGAC
    TTCCGCGCCC TTGTCACCAA CAAGCCGGTG GTCTCGGCCC TGCACGGGGC CGTGCCAGGC
    CGTTTCCAGA AAGACATCTG TATAGGGAAC CAGTCCAACC CCTGTGTGCC TAACAACAAA
    AACCCCAGGG CTTTCAATGG CTCCAGCAAT GGGCATGTTT ATGAGAAACT TTCTAGTATT
    GAGTCTGATG TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe43518
    Clone ID Related Accession (Same CDS sequence) XM_014108269.1 , XM_014108270.1
    Accession Version XM_014108270.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4035bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product glutamate receptor ionotropic, NMDA 2B isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTTCTTCC AGTTTGGCCC ATCAATTGAA CAGCAAGCTT CTGTAATGCT CAATATCATG 
    GAAGAGTATG ACTGGTACAT CTTTTCTATT GTCACCACCT ACTTCCCTGG CTACCAGGAC
    TTTGTAAACA AGATTCGCAG CACCATTGAG AACAGCTTCG TGGGCTGGGA GTTAGAGGAA
    GTCCTCCTGC TGGACATGTC CCTGGATGAT GGAGATTCTA AGATCCAGAA CCAGCTCAAG
    AAACTGCAGA GCCCCATCAT TCTTCTCTAT TGTACTAAGG AAGAAGCCAC GTATATCTTT
    GAAGTAGCTA ACTCAGTAGG GCTGACTGGC TACGGCTACA CATGGATTGT ACCTAGTCTG
    GTGGCAGGGG ATACAGACAC AGTGCCCTCG GAGTTCCCCA CTGGCCTCAT TTCTGTGTCC
    TATGATGAAT GGGACTATGG CCTCCCTGCC AGAGTGAGGG ATGGAATTGC CATAATCACC
    ACTGCTGCTT CCGACATGCT GTCTGAACAC AGCTTCATCC CTGAGCCCAA GAGCAGTTGT
    TACAACACCC ATGAGAAGAG AATCTACCAG TCCAACATGC TAAATAGGTA TCTGATCAAT
    GTCACTTTTG AGGGAAGAAA TCTGTCCTTC AGTGAAGATG GCTACCAGAT GCACCCGAAA
    CTGGTGATAA TCCTTCTGAA CAAGGAGAGA AAGTGGGAGA GGGTGGGGAA GTGGAAAGAC
    AAATCCTTGC AGATGAAGTA CTACGTGTGG CCCCGAATGT GTCCTGAGAC AGAAGAACAG
    GAGGATGACC ATCTGAGCAT TGTAACCCTG GAGGAGGCCC CCTTTGTCAT TGTGGAGAGT
    GTGGACCCTC TGAGTGGAAC CTGCATGAGG AACACAGTCC CCTGCCAAAA ACGCATAGTC
    TCTGAGAATA AAACAGATGA AGAGCCAGGT TACATCAAAA AGTGCTGCAA GGGGTTCTGT
    ATTGACATCC TTAAGAAAAT TTCTAAATCT GTGAAGTTCA CCTATGATCT CTACCTGGTT
    ACCAATGGCA AGCATGGGAA GAAAATCAAT GGAACTTGGA ATGGTATGAT TGGAGAGGTG
    GTTATGAAGA GGGCTTACAT GGCGGTGGGA TCACTCACCA TCAATGAGGA GCGATCTGAG
    GTGGTTGACT TCTCTGTGCC CTTCATAGAG ACTGGCATCA GTGTTATGGT GTCACGCAGC
    AATGGGACTG TCTCACCATC TGCCTTCTTA GAGCCATTCA GTGCTGACGT ATGGGTTATG
    ATGTTCGTGA TGCTGCTCAT CGTCTCAGCC GTGGCTGTCT TTGTCTTCGA GTACTTCAGC
    CCTGTGGGTT ACAACAGGTG CCTCGCTGAT GGCAGAGAGC CAGGTGGCCC GTCTTTTACC
    ATCGGCAAAG CTATTTGGTT ACTGTGGGGT CTGGTGTTTA ACAACTCCGT ACCCGTGCAG
    AACCCAAAGG GGACCACTTC CAAGATCATG GTGTCGGTGT GGGCCTTCTT TGCCGTCATC
    TTCCTGGCCA GCTACACTGC CAACTTAGCT GCCTTCATGA TCCAAGAAGA GTATGTGGAC
    CAGGTTTCTG GCCTGAGTGA CAAAAAGTTC CAGAGACCTA ATGACTTCTC ACCCCCTTTT
    CGCTTTGGGA CTGTGCCCAA TGGCAGCACA GAGAGGAATA TTCGCAATAA CTACGCAGAA
    ATGCATGCCT ACATGGGAAA GTTCAACCAG AGGGGTGTAG ATGATGCATT GCTCTCCCTG
    AAAACAGGGA AACTGGATGC CTTCATCTAT GATGCAGCAG TGCTGAACTA CATGGCGGGC
    AGAGATGAAG GCTGCAAGCT GGTGACCATT GGCAGTGGGA AAGTCTTTGC TTCCACTGGT
    TATGGCATTG CCATCCAAAA AGATTCTGGG TGGAAGCGCC AGGTGGACCT TGCTATCCTC
    CAGCTATTCG GAGATGGGGA GATGGAGGAG CTAGAAGCTC TTTGGCTCAC TGGCATTTGC
    CACAATGAAA AGAATGAGGT CATGAGCAGC CAGCTGGACA TTGACAACAT GGCAGGAGTC
    TTCTACATGT TGGGGGCAGC CATGGCTCTC AGTCTCATCA CCTTCATCTG TGAACACCTT
    TTCTATTGGC AGTTCCGGCA CTGCTTTATG GGTGTCTGTT CCGGCAAGCC TGGCATGGTC
    TTCTCCATCA GCAGAGGTAT CTACAGTTGC ATCCATGGAG TGGCTATCGA GGAGCGCCAG
    TCCGTGATGA ATTCCCCCAC CGCGACCATG AACAACACAC ACTCCAACAT CCTGCGCCTG
    CTCCGCACGG CCAAGAACAT GGCCAACCTG TCTGGTGTGA ACGGCTCCCC CCAGAGTGCC
    CTGGACTTCA TCCGACGTGA GTCGTCCGTC TATGACATCT CCGAGCACCG CCGCAGCTTC
    ACGCATTCCG ACTGCAAATC CTACAACAAC CCGCCGTGCG AGGAGAACCT CTTCAGCGAC
    TACATCAGTG AGGTGGAGAG GACTTTTGGT AACCTGCAGC TGAAGGACAG CAACGTGTAC
    CAAGATCACT ATCACCACCA CCACCGGCCC CACAGCATCG GCAGCGCCAG CTCCATCGAC
    GGGCTCTACG ACTGTGACAA CCCGCCTTTC ACCACCCAGC CGAGGTCCAT CAGCAAGAAG
    CCCCTGGACC TCGGCCTCCC CTCCTCCAAA CACAGCCAGC TCAGTGACCT GTACGGCAAA
    TTCTCCTTCA AGAGCGACCG CTACAGTGGC CACGATGACT TGATCCGCTC GGACGTGTCT
    GACATCTCCA CGCACACGGT CACCTACGGA AACATAGAGG GCAACGCCGC CAAGAGGCGC
    AAGCAGCAAT ACAAGGACAG CCTGAAGAAG CGGCCGGCCT CGGCCAAGTC CCGGCGGGAG
    TTCGACGAGA TCGAGCTGGC CTACCGCCGC CGGCCGCCCC GCTCCCCTGA CCACAAGCGC
    TACTTCAGGG ACAAGGAGGG GCTCCGGGAC TTCTACCTGG ACCAGTTCCG CACCAAGGAG
    AACTCCCCGC ACTGGGAGCA CGTGGACCTG ACGGACATCT ACAAGGAGCA GAGCGATGAC
    TTTAAGCGCG ACTCGGTGAG CGGAGGAGGG CCCTGTACCA ACAGGTCTCA CCTCAAACAC
    GGGGCGGGCG ACAAGCACGG CGTGGTCAGC GGGGTCCCGG CCCCTTGGGA GAAGAACCTG
    AGCAACGTGG ATTGGGAGGA CCGCTCCGGG GGCAACTTTT GCCGCAGCTG CCCCTCAAAG
    CTGCACAACT ACTCGACGGC GGTGACCGGG CAGAACTCGG GCCGGCAGGC GTGCATCCGG
    TGCGAGGCCT GCAAGAAGGC AGGGAACCTG TACGACATCA GCGAGGACAA CTCCCTGCAG
    GAGCTGGACC AGCCGGCTGC CCCTGTGGCG GTGCCGTCAA ACGCGCCCAG CACCAAGTAC
    CCCCAGAGCC CGACCAACTC CAAGGCCCAG AAGAAGACCC GGAACAAGCT GCGCCGGCAG
    CACTCCTACG ACACCTTCGT GGACCTGCAG AAGGAAGAAG CCGCCTTGGC CCCGCGCAGC
    GTGAGCCTGA AAGACAAGGG CCGGTTCCTG GACGGGAGTC CCTACGCCCA CATGTTTGAG
    ACGCCAGCTG GCGAGAGCAC CTTTGCCAAC CACGAGTCCT CAGTGGCCGC CGCCGGACAC
    CGCCACCACA ACAACCCCGG CGGCGGCGGC TACATGCTCA GCAAGTCGCT CTACCCCGAC
    CGGGTCACGC AAAACCCTTT CATCCCCACT TTTGGGGACG ACCAGTGCTT GCTCCATGGC
    AGCAAATCCT ACTTCTTCAG GCAGCCCACG GTGGCGGGGG CGCCGAAAGC CAGGCCAGAC
    TTCCGCGCCC TTGTCACCAA CAAGCCGGTG GTCTCGGCCC TGCACGGGGC CGTGCCAGGC
    CGTTTCCAGA AAGACATCTG TATAGGGAAC CAGTCCAACC CCTGTGTGCC TAACAACAAA
    AACCCCAGGG CTTTCAATGG CTCCAGCAAT GGGCATGTTT ATGAGAAACT TTCTAGTATT
    GAGTCTGATG TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963745.1
    CDS79..4113
    Translation

    Target ORF information:

    RefSeq Version XM_014108270.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris glutamate receptor, ionotropic, N-methyl D-aspartate 2B (GRIN2B), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014108270.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGTTCTTCC AGTTTGGCCC ATCAATTGAA CAGCAAGCTT CTGTAATGCT CAATATCATG 
    GAAGAGTATG ACTGGTACAT CTTTTCTATT GTCACCACCT ACTTCCCTGG CTACCAGGAC
    TTTGTAAACA AGATTCGCAG CACCATTGAG AACAGCTTCG TGGGCTGGGA GTTAGAGGAA
    GTCCTCCTGC TGGACATGTC CCTGGATGAT GGAGATTCTA AGATCCAGAA CCAGCTCAAG
    AAACTGCAGA GCCCCATCAT TCTTCTCTAT TGTACTAAGG AAGAAGCCAC GTATATCTTT
    GAAGTAGCTA ACTCAGTAGG GCTGACTGGC TACGGCTACA CATGGATTGT ACCTAGTCTG
    GTGGCAGGGG ATACAGACAC AGTGCCCTCG GAGTTCCCCA CTGGCCTCAT TTCTGTGTCC
    TATGATGAAT GGGACTATGG CCTCCCTGCC AGAGTGAGGG ATGGAATTGC CATAATCACC
    ACTGCTGCTT CCGACATGCT GTCTGAACAC AGCTTCATCC CTGAGCCCAA GAGCAGTTGT
    TACAACACCC ATGAGAAGAG AATCTACCAG TCCAACATGC TAAATAGGTA TCTGATCAAT
    GTCACTTTTG AGGGAAGAAA TCTGTCCTTC AGTGAAGATG GCTACCAGAT GCACCCGAAA
    CTGGTGATAA TCCTTCTGAA CAAGGAGAGA AAGTGGGAGA GGGTGGGGAA GTGGAAAGAC
    AAATCCTTGC AGATGAAGTA CTACGTGTGG CCCCGAATGT GTCCTGAGAC AGAAGAACAG
    GAGGATGACC ATCTGAGCAT TGTAACCCTG GAGGAGGCCC CCTTTGTCAT TGTGGAGAGT
    GTGGACCCTC TGAGTGGAAC CTGCATGAGG AACACAGTCC CCTGCCAAAA ACGCATAGTC
    TCTGAGAATA AAACAGATGA AGAGCCAGGT TACATCAAAA AGTGCTGCAA GGGGTTCTGT
    ATTGACATCC TTAAGAAAAT TTCTAAATCT GTGAAGTTCA CCTATGATCT CTACCTGGTT
    ACCAATGGCA AGCATGGGAA GAAAATCAAT GGAACTTGGA ATGGTATGAT TGGAGAGGTG
    GTTATGAAGA GGGCTTACAT GGCGGTGGGA TCACTCACCA TCAATGAGGA GCGATCTGAG
    GTGGTTGACT TCTCTGTGCC CTTCATAGAG ACTGGCATCA GTGTTATGGT GTCACGCAGC
    AATGGGACTG TCTCACCATC TGCCTTCTTA GAGCCATTCA GTGCTGACGT ATGGGTTATG
    ATGTTCGTGA TGCTGCTCAT CGTCTCAGCC GTGGCTGTCT TTGTCTTCGA GTACTTCAGC
    CCTGTGGGTT ACAACAGGTG CCTCGCTGAT GGCAGAGAGC CAGGTGGCCC GTCTTTTACC
    ATCGGCAAAG CTATTTGGTT ACTGTGGGGT CTGGTGTTTA ACAACTCCGT ACCCGTGCAG
    AACCCAAAGG GGACCACTTC CAAGATCATG GTGTCGGTGT GGGCCTTCTT TGCCGTCATC
    TTCCTGGCCA GCTACACTGC CAACTTAGCT GCCTTCATGA TCCAAGAAGA GTATGTGGAC
    CAGGTTTCTG GCCTGAGTGA CAAAAAGTTC CAGAGACCTA ATGACTTCTC ACCCCCTTTT
    CGCTTTGGGA CTGTGCCCAA TGGCAGCACA GAGAGGAATA TTCGCAATAA CTACGCAGAA
    ATGCATGCCT ACATGGGAAA GTTCAACCAG AGGGGTGTAG ATGATGCATT GCTCTCCCTG
    AAAACAGGGA AACTGGATGC CTTCATCTAT GATGCAGCAG TGCTGAACTA CATGGCGGGC
    AGAGATGAAG GCTGCAAGCT GGTGACCATT GGCAGTGGGA AAGTCTTTGC TTCCACTGGT
    TATGGCATTG CCATCCAAAA AGATTCTGGG TGGAAGCGCC AGGTGGACCT TGCTATCCTC
    CAGCTATTCG GAGATGGGGA GATGGAGGAG CTAGAAGCTC TTTGGCTCAC TGGCATTTGC
    CACAATGAAA AGAATGAGGT CATGAGCAGC CAGCTGGACA TTGACAACAT GGCAGGAGTC
    TTCTACATGT TGGGGGCAGC CATGGCTCTC AGTCTCATCA CCTTCATCTG TGAACACCTT
    TTCTATTGGC AGTTCCGGCA CTGCTTTATG GGTGTCTGTT CCGGCAAGCC TGGCATGGTC
    TTCTCCATCA GCAGAGGTAT CTACAGTTGC ATCCATGGAG TGGCTATCGA GGAGCGCCAG
    TCCGTGATGA ATTCCCCCAC CGCGACCATG AACAACACAC ACTCCAACAT CCTGCGCCTG
    CTCCGCACGG CCAAGAACAT GGCCAACCTG TCTGGTGTGA ACGGCTCCCC CCAGAGTGCC
    CTGGACTTCA TCCGACGTGA GTCGTCCGTC TATGACATCT CCGAGCACCG CCGCAGCTTC
    ACGCATTCCG ACTGCAAATC CTACAACAAC CCGCCGTGCG AGGAGAACCT CTTCAGCGAC
    TACATCAGTG AGGTGGAGAG GACTTTTGGT AACCTGCAGC TGAAGGACAG CAACGTGTAC
    CAAGATCACT ATCACCACCA CCACCGGCCC CACAGCATCG GCAGCGCCAG CTCCATCGAC
    GGGCTCTACG ACTGTGACAA CCCGCCTTTC ACCACCCAGC CGAGGTCCAT CAGCAAGAAG
    CCCCTGGACC TCGGCCTCCC CTCCTCCAAA CACAGCCAGC TCAGTGACCT GTACGGCAAA
    TTCTCCTTCA AGAGCGACCG CTACAGTGGC CACGATGACT TGATCCGCTC GGACGTGTCT
    GACATCTCCA CGCACACGGT CACCTACGGA AACATAGAGG GCAACGCCGC CAAGAGGCGC
    AAGCAGCAAT ACAAGGACAG CCTGAAGAAG CGGCCGGCCT CGGCCAAGTC CCGGCGGGAG
    TTCGACGAGA TCGAGCTGGC CTACCGCCGC CGGCCGCCCC GCTCCCCTGA CCACAAGCGC
    TACTTCAGGG ACAAGGAGGG GCTCCGGGAC TTCTACCTGG ACCAGTTCCG CACCAAGGAG
    AACTCCCCGC ACTGGGAGCA CGTGGACCTG ACGGACATCT ACAAGGAGCA GAGCGATGAC
    TTTAAGCGCG ACTCGGTGAG CGGAGGAGGG CCCTGTACCA ACAGGTCTCA CCTCAAACAC
    GGGGCGGGCG ACAAGCACGG CGTGGTCAGC GGGGTCCCGG CCCCTTGGGA GAAGAACCTG
    AGCAACGTGG ATTGGGAGGA CCGCTCCGGG GGCAACTTTT GCCGCAGCTG CCCCTCAAAG
    CTGCACAACT ACTCGACGGC GGTGACCGGG CAGAACTCGG GCCGGCAGGC GTGCATCCGG
    TGCGAGGCCT GCAAGAAGGC AGGGAACCTG TACGACATCA GCGAGGACAA CTCCCTGCAG
    GAGCTGGACC AGCCGGCTGC CCCTGTGGCG GTGCCGTCAA ACGCGCCCAG CACCAAGTAC
    CCCCAGAGCC CGACCAACTC CAAGGCCCAG AAGAAGACCC GGAACAAGCT GCGCCGGCAG
    CACTCCTACG ACACCTTCGT GGACCTGCAG AAGGAAGAAG CCGCCTTGGC CCCGCGCAGC
    GTGAGCCTGA AAGACAAGGG CCGGTTCCTG GACGGGAGTC CCTACGCCCA CATGTTTGAG
    ACGCCAGCTG GCGAGAGCAC CTTTGCCAAC CACGAGTCCT CAGTGGCCGC CGCCGGACAC
    CGCCACCACA ACAACCCCGG CGGCGGCGGC TACATGCTCA GCAAGTCGCT CTACCCCGAC
    CGGGTCACGC AAAACCCTTT CATCCCCACT TTTGGGGACG ACCAGTGCTT GCTCCATGGC
    AGCAAATCCT ACTTCTTCAG GCAGCCCACG GTGGCGGGGG CGCCGAAAGC CAGGCCAGAC
    TTCCGCGCCC TTGTCACCAA CAAGCCGGTG GTCTCGGCCC TGCACGGGGC CGTGCCAGGC
    CGTTTCCAGA AAGACATCTG TATAGGGAAC CAGTCCAACC CCTGTGTGCC TAACAACAAA
    AACCCCAGGG CTTTCAATGG CTCCAGCAAT GGGCATGTTT ATGAGAAACT TTCTAGTATT
    GAGTCTGATG TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26828
    Clone ID Related Accession (Same CDS sequence) XM_022410865.1
    Accession Version XM_022410865.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4542bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product glutamate receptor ionotropic, NMDA 2B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAAGCCCA GAGCAGAGTG CTGTTCTCCC AAGTTCTGGT TGGTGTTGGC TGTCCTGGCT 
    GTGTCAGGCA GCAGAGCTCG TTCTCAGAAG AGCCCCCCCA GCATTGGCAT TGCTGTCATC
    CTCGTGGGCA CTTCAGACGA GGTGGCCATC AAGGATGCCC ACGAGAAAGA TGATTTCCAC
    CATCTCTCTG TGGTTCCCCG TGTGGAGCTG GTAGCCATGA ATGAGACTGA CCCAAAGAGC
    ATCATCACCC GCATCTGTGA TCTCATGTCT GACCGGAAGA TCCAGGGCGT GGTGTTTGCT
    GACGACACAG ATCAGGAGGC CATTGCCCAG ATCCTTGACT TCATTTCAGC CCAGACTCTC
    ACCCCCATCC TGGGCATCCA CGGAGGCTCC TCTATGATAA TGGCAGATAA GGATGAATCC
    TCCATGTTCT TCCAGTTTGG CCCATCAATT GAACAGCAAG CTTCTGTAAT GCTCAATATC
    ATGGAAGAGT ATGACTGGTA CATCTTTTCT ATTGTCACCA CCTACTTCCC TGGCTACCAG
    GACTTTGTAA ACAAGATTCG CAGCACCATT GAGAACAGCT TCGTGGGCTG GGAGTTAGAG
    GAAGTCCTCC TGCTGGACAT GTCCCTGGAT GATGGAGATT CTAAGATCCA GAACCAGCTC
    AAGAAACTGC AGAGCCCCAT CATTCTTCTC TATTGTACTA AGGAAGAAGC CACGTATATC
    TTTGAAGTAG CTAACTCAGT AGGGCTGACT GGCTACGGCT ACACATGGAT TGTACCTAGT
    CTGGTGGCAG GGGATACAGA CACAGTGCCC TCGGAGTTCC CCACTGGCCT CATTTCTGTG
    TCCTATGATG AATGGGACTA TGGCCTCCCT GCCAGAGTGA GGGATGGAAT TGCCATAATC
    ACCACTGCTG CTTCCGACAT GCTGTCTGAA CACAGCTTCA TCCCTGAGCC CAAGAGCAGT
    TGTTACAACA CCCATGAGAA GAGAATCTAC CAGTCCAACA TGCTAAATAG ATGCAGAGAC
    AGTAAAGATA ACAAGATTTG GAGAAAAAAG AATAACCAAA AGAAGGGACC ATTTCACAGG
    GCTGTTGATT TTGTGTATCT GATCAATGTC ACTTTTGAGG GAAGAAATCT GTCCTTCAGT
    GAAGATGGCT ACCAGATGCA CCCGAAACTG GTGATAATCC TTCTGAACAA GGAGAGAAAG
    TGGGAGAGGG TGGGGAAGTG GAAAGACAAA TCCTTGCAGA TGAAGTACTA CGTGTGGCCC
    CGAATGTGTC CTGAGACAGA AGAACAGGAG GATGACCATC TGAGCATTGT AACCCTGGAG
    GAGGCCCCCT TTGTCATTGT GGAGAGTGTG GACCCTCTGA GTGGAACCTG CATGAGGAAC
    ACAGTCCCCT GCCAAAAACG CATAGTCTCT GAGAATAAAA CAGATGAAGA GCCAGGTTAC
    ATCAAAAAGT GCTGCAAGGG GTTCTGTATT GACATCCTTA AGAAAATTTC TAAATCTGTG
    AAGTTCACCT ATGATCTCTA CCTGGTTACC AATGGCAAGC ATGGGAAGAA AATCAATGGA
    ACTTGGAATG GTATGATTGG AGAGGTGGTT ATGAAGAGGG CTTACATGGC GGTGGGATCA
    CTCACCATCA ATGAGGAGCG ATCTGAGGTG GTTGACTTCT CTGTGCCCTT CATAGAGACT
    GGCATCAGTG TTATGGTGTC ACGCAGCAAT GGGACTGTCT CACCATCTGC CTTCTTAGAG
    CCATTCAGTG CTGACGTATG GGTTATGATG TTCGTGATGC TGCTCATCGT CTCAGCCGTG
    GCTGTCTTTG TCTTCGAGTA CTTCAGCCCT GTGGGTTACA ACAGGTGCCT CGCTGATGGC
    AGAGAGCCAG GTGGCCCGTC TTTTACCATC GGCAAAGCTA TTTGGTTACT GTGGGGTCTG
    GTGTTTAACA ACTCCGTACC CGTGCAGAAC CCAAAGGGGA CCACTTCCAA GATCATGGTG
    TCGGTGTGGG CCTTCTTTGC CGTCATCTTC CTGGCCAGCT ACACTGCCAA CTTAGCTGCC
    TTCATGATCC AAGAAGAGTA TGTGGACCAG GTTTCTGGCC TGAGTGACAA AAAGTTCCAG
    AGACCTAATG ACTTCTCACC CCCTTTTCGC TTTGGGACTG TGCCCAATGG CAGCACAGAG
    AGGAATATTC GCAATAACTA CGCAGAAATG CATGCCTACA TGGGAAAGTT CAACCAGAGG
    GGTGTAGATG ATGCATTGCT CTCCCTGAAA ACAGGGAAAC TGGATGCCTT CATCTATGAT
    GCAGCAGTGC TGAACTACAT GGCGGGCAGA GATGAAGGCT GCAAGCTGGT GACCATTGGC
    AGTGGGAAAG TCTTTGCTTC CACTGGTTAT GGCATTGCCA TCCAAAAAGA TTCTGGGTGG
    AAGCGCCAGG TGGACCTTGC TATCCTCCAG CTATTCGGAG ATGGGGAGAT GGAGGAGCTA
    GAAGCTCTTT GGCTCACTGG CATTTGCCAC AATGAAAAGA ATGAGGTCAT GAGCAGCCAG
    CTGGACATTG ACAACATGGC AGGAGTCTTC TACATGTTGG GGGCAGCCAT GGCTCTCAGT
    CTCATCACCT TCATCTGTGA ACACCTTTTC TATTGGCAGT TCCGGCACTG CTTTATGGGT
    GTCTGTTCCG GCAAGCCTGG CATGGTCTTC TCCATCAGCA GAGGTATCTA CAGTTGCATC
    CATGGAGTGG CTATCGAGGA GCGCCAGTCC GTGATGAATT CCCCCACCGC GACCATGAAC
    AACACACACT CCAACATCCT GCGCCTGCTC CGCACGGCCA AGAACATGGC CAACCTGTCT
    GGTGTGAACG GCTCCCCCCA GAGTGCCCTG GACTTCATCC GACGTGAGTC GTCCGTCTAT
    GACATCTCCG AGCACCGCCG CAGCTTCACG CATTCCGACT GCAAATCCTA CAACAACCCG
    CCGTGCGAGG AGAACCTCTT CAGCGACTAC ATCAGTGAGG TGGAGAGGAC TTTTGGTAAC
    CTGCAGCTGA AGGACAGCAA CGTGTACCAA GATCACTATC ACCACCACCA CCGGCCCCAC
    AGCATCGGCA GCGCCAGCTC CATCGACGGG CTCTACGACT GTGACAACCC GCCTTTCACC
    ACCCAGCCGA GGTCCATCAG CAAGAAGCCC CTGGACCTCG GCCTCCCCTC CTCCAAACAC
    AGCCAGCTCA GTGACCTGTA CGGCAAATTC TCCTTCAAGA GCGACCGCTA CAGTGGCCAC
    GATGACTTGA TCCGCTCGGA CGTGTCTGAC ATCTCCACGC ACACGGTCAC CTACGGAAAC
    ATAGAGGGCA ACGCCGCCAA GAGGCGCAAG CAGCAATACA AGGACAGCCT GAAGAAGCGG
    CCGGCCTCGG CCAAGTCCCG GCGGGAGTTC GACGAGATCG AGCTGGCCTA CCGCCGCCGG
    CCGCCCCGCT CCCCTGACCA CAAGCGCTAC TTCAGGGACA AGGAGGGGCT CCGGGACTTC
    TACCTGGACC AGTTCCGCAC CAAGGAGAAC TCCCCGCACT GGGAGCACGT GGACCTGACG
    GACATCTACA AGGAGCAGAG CGATGACTTT AAGCGCGACT CGGTGAGCGG AGGAGGGCCC
    TGTACCAACA GGTCTCACCT CAAACACGGG GCGGGCGACA AGCACGGCGT GGTCAGCGGG
    GTCCCGGCCC CTTGGGAGAA GAACCTGAGC AACGTGGATT GGGAGGACCG CTCCGGGGGC
    AACTTTTGCC GCAGCTGCCC CTCAAAGCTG CACAACTACT CGACGGCGGT GACCGGGCAG
    AACTCGGGCC GGCAGGCGTG CATCCGGTGC GAGGCCTGCA AGAAGGCAGG GAACCTGTAC
    GACATCAGCG AGGACAACTC CCTGCAGGAG CTGGACCAGC CGGCTGCCCC TGTGGCGGTG
    CCGTCAAACG CGCCCAGCAC CAAGTACCCC CAGAGCCCGA CCAACTCCAA GGCCCAGAAG
    AAGACCCGGA ACAAGCTGCG CCGGCAGCAC TCCTACGACA CCTTCGTGGA CCTGCAGAAG
    GAAGAAGCCG CCTTGGCCCC GCGCAGCGTG AGCCTGAAAG ACAAGGGCCG GTTCCTGGAC
    GGGAGTCCCT ACGCCCACAT GTTTGAGACG CCAGCTGGCG AGAGCACCTT TGCCAACCAC
    GAGTCCTCAG TGGCCGCCGC CGGACACCGC CACCACAACA ACCCCGGCGG CGGCGGCTAC
    ATGCTCAGCA AGTCGCTCTA CCCCGACCGG GTCACGCAAA ACCCTTTCAT CCCCACTTTT
    GGGGACGACC AGTGCTTGCT CCATGGCAGC AAATCCTACT TCTTCAGGCA GCCCACGGTG
    GCGGGGGCGC CGAAAGCCAG GCCAGACTTC CGCGCCCTTG TCACCAACAA GCCGGTGGTC
    TCGGCCCTGC ACGGGGCCGT GCCAGGCCGT TTCCAGAAAG ACATCTGTAT AGGGAACCAG
    TCCAACCCCT GTGTGCCTAA CAACAAAAAC CCCAGGGCTT TCAATGGCTC CAGCAATGGG
    CATGTTTATG AGAAACTTTC TAGTATTGAG TCTGATGTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022266573.1
    CDS718..5259
    Translation

    Target ORF information:

    RefSeq Version XM_022410865.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris glutamate ionotropic receptor NMDA type subunit 2B (GRIN2B), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022410865.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAAGCCCA GAGCAGAGTG CTGTTCTCCC AAGTTCTGGT TGGTGTTGGC TGTCCTGGCT 
    GTGTCAGGCA GCAGAGCTCG TTCTCAGAAG AGCCCCCCCA GCATTGGCAT TGCTGTCATC
    CTCGTGGGCA CTTCAGACGA GGTGGCCATC AAGGATGCCC ACGAGAAAGA TGATTTCCAC
    CATCTCTCTG TGGTTCCCCG TGTGGAGCTG GTAGCCATGA ATGAGACTGA CCCAAAGAGC
    ATCATCACCC GCATCTGTGA TCTCATGTCT GACCGGAAGA TCCAGGGCGT GGTGTTTGCT
    GACGACACAG ATCAGGAGGC CATTGCCCAG ATCCTTGACT TCATTTCAGC CCAGACTCTC
    ACCCCCATCC TGGGCATCCA CGGAGGCTCC TCTATGATAA TGGCAGATAA GGATGAATCC
    TCCATGTTCT TCCAGTTTGG CCCATCAATT GAACAGCAAG CTTCTGTAAT GCTCAATATC
    ATGGAAGAGT ATGACTGGTA CATCTTTTCT ATTGTCACCA CCTACTTCCC TGGCTACCAG
    GACTTTGTAA ACAAGATTCG CAGCACCATT GAGAACAGCT TCGTGGGCTG GGAGTTAGAG
    GAAGTCCTCC TGCTGGACAT GTCCCTGGAT GATGGAGATT CTAAGATCCA GAACCAGCTC
    AAGAAACTGC AGAGCCCCAT CATTCTTCTC TATTGTACTA AGGAAGAAGC CACGTATATC
    TTTGAAGTAG CTAACTCAGT AGGGCTGACT GGCTACGGCT ACACATGGAT TGTACCTAGT
    CTGGTGGCAG GGGATACAGA CACAGTGCCC TCGGAGTTCC CCACTGGCCT CATTTCTGTG
    TCCTATGATG AATGGGACTA TGGCCTCCCT GCCAGAGTGA GGGATGGAAT TGCCATAATC
    ACCACTGCTG CTTCCGACAT GCTGTCTGAA CACAGCTTCA TCCCTGAGCC CAAGAGCAGT
    TGTTACAACA CCCATGAGAA GAGAATCTAC CAGTCCAACA TGCTAAATAG ATGCAGAGAC
    AGTAAAGATA ACAAGATTTG GAGAAAAAAG AATAACCAAA AGAAGGGACC ATTTCACAGG
    GCTGTTGATT TTGTGTATCT GATCAATGTC ACTTTTGAGG GAAGAAATCT GTCCTTCAGT
    GAAGATGGCT ACCAGATGCA CCCGAAACTG GTGATAATCC TTCTGAACAA GGAGAGAAAG
    TGGGAGAGGG TGGGGAAGTG GAAAGACAAA TCCTTGCAGA TGAAGTACTA CGTGTGGCCC
    CGAATGTGTC CTGAGACAGA AGAACAGGAG GATGACCATC TGAGCATTGT AACCCTGGAG
    GAGGCCCCCT TTGTCATTGT GGAGAGTGTG GACCCTCTGA GTGGAACCTG CATGAGGAAC
    ACAGTCCCCT GCCAAAAACG CATAGTCTCT GAGAATAAAA CAGATGAAGA GCCAGGTTAC
    ATCAAAAAGT GCTGCAAGGG GTTCTGTATT GACATCCTTA AGAAAATTTC TAAATCTGTG
    AAGTTCACCT ATGATCTCTA CCTGGTTACC AATGGCAAGC ATGGGAAGAA AATCAATGGA
    ACTTGGAATG GTATGATTGG AGAGGTGGTT ATGAAGAGGG CTTACATGGC GGTGGGATCA
    CTCACCATCA ATGAGGAGCG ATCTGAGGTG GTTGACTTCT CTGTGCCCTT CATAGAGACT
    GGCATCAGTG TTATGGTGTC ACGCAGCAAT GGGACTGTCT CACCATCTGC CTTCTTAGAG
    CCATTCAGTG CTGACGTATG GGTTATGATG TTCGTGATGC TGCTCATCGT CTCAGCCGTG
    GCTGTCTTTG TCTTCGAGTA CTTCAGCCCT GTGGGTTACA ACAGGTGCCT CGCTGATGGC
    AGAGAGCCAG GTGGCCCGTC TTTTACCATC GGCAAAGCTA TTTGGTTACT GTGGGGTCTG
    GTGTTTAACA ACTCCGTACC CGTGCAGAAC CCAAAGGGGA CCACTTCCAA GATCATGGTG
    TCGGTGTGGG CCTTCTTTGC CGTCATCTTC CTGGCCAGCT ACACTGCCAA CTTAGCTGCC
    TTCATGATCC AAGAAGAGTA TGTGGACCAG GTTTCTGGCC TGAGTGACAA AAAGTTCCAG
    AGACCTAATG ACTTCTCACC CCCTTTTCGC TTTGGGACTG TGCCCAATGG CAGCACAGAG
    AGGAATATTC GCAATAACTA CGCAGAAATG CATGCCTACA TGGGAAAGTT CAACCAGAGG
    GGTGTAGATG ATGCATTGCT CTCCCTGAAA ACAGGGAAAC TGGATGCCTT CATCTATGAT
    GCAGCAGTGC TGAACTACAT GGCGGGCAGA GATGAAGGCT GCAAGCTGGT GACCATTGGC
    AGTGGGAAAG TCTTTGCTTC CACTGGTTAT GGCATTGCCA TCCAAAAAGA TTCTGGGTGG
    AAGCGCCAGG TGGACCTTGC TATCCTCCAG CTATTCGGAG ATGGGGAGAT GGAGGAGCTA
    GAAGCTCTTT GGCTCACTGG CATTTGCCAC AATGAAAAGA ATGAGGTCAT GAGCAGCCAG
    CTGGACATTG ACAACATGGC AGGAGTCTTC TACATGTTGG GGGCAGCCAT GGCTCTCAGT
    CTCATCACCT TCATCTGTGA ACACCTTTTC TATTGGCAGT TCCGGCACTG CTTTATGGGT
    GTCTGTTCCG GCAAGCCTGG CATGGTCTTC TCCATCAGCA GAGGTATCTA CAGTTGCATC
    CATGGAGTGG CTATCGAGGA GCGCCAGTCC GTGATGAATT CCCCCACCGC GACCATGAAC
    AACACACACT CCAACATCCT GCGCCTGCTC CGCACGGCCA AGAACATGGC CAACCTGTCT
    GGTGTGAACG GCTCCCCCCA GAGTGCCCTG GACTTCATCC GACGTGAGTC GTCCGTCTAT
    GACATCTCCG AGCACCGCCG CAGCTTCACG CATTCCGACT GCAAATCCTA CAACAACCCG
    CCGTGCGAGG AGAACCTCTT CAGCGACTAC ATCAGTGAGG TGGAGAGGAC TTTTGGTAAC
    CTGCAGCTGA AGGACAGCAA CGTGTACCAA GATCACTATC ACCACCACCA CCGGCCCCAC
    AGCATCGGCA GCGCCAGCTC CATCGACGGG CTCTACGACT GTGACAACCC GCCTTTCACC
    ACCCAGCCGA GGTCCATCAG CAAGAAGCCC CTGGACCTCG GCCTCCCCTC CTCCAAACAC
    AGCCAGCTCA GTGACCTGTA CGGCAAATTC TCCTTCAAGA GCGACCGCTA CAGTGGCCAC
    GATGACTTGA TCCGCTCGGA CGTGTCTGAC ATCTCCACGC ACACGGTCAC CTACGGAAAC
    ATAGAGGGCA ACGCCGCCAA GAGGCGCAAG CAGCAATACA AGGACAGCCT GAAGAAGCGG
    CCGGCCTCGG CCAAGTCCCG GCGGGAGTTC GACGAGATCG AGCTGGCCTA CCGCCGCCGG
    CCGCCCCGCT CCCCTGACCA CAAGCGCTAC TTCAGGGACA AGGAGGGGCT CCGGGACTTC
    TACCTGGACC AGTTCCGCAC CAAGGAGAAC TCCCCGCACT GGGAGCACGT GGACCTGACG
    GACATCTACA AGGAGCAGAG CGATGACTTT AAGCGCGACT CGGTGAGCGG AGGAGGGCCC
    TGTACCAACA GGTCTCACCT CAAACACGGG GCGGGCGACA AGCACGGCGT GGTCAGCGGG
    GTCCCGGCCC CTTGGGAGAA GAACCTGAGC AACGTGGATT GGGAGGACCG CTCCGGGGGC
    AACTTTTGCC GCAGCTGCCC CTCAAAGCTG CACAACTACT CGACGGCGGT GACCGGGCAG
    AACTCGGGCC GGCAGGCGTG CATCCGGTGC GAGGCCTGCA AGAAGGCAGG GAACCTGTAC
    GACATCAGCG AGGACAACTC CCTGCAGGAG CTGGACCAGC CGGCTGCCCC TGTGGCGGTG
    CCGTCAAACG CGCCCAGCAC CAAGTACCCC CAGAGCCCGA CCAACTCCAA GGCCCAGAAG
    AAGACCCGGA ACAAGCTGCG CCGGCAGCAC TCCTACGACA CCTTCGTGGA CCTGCAGAAG
    GAAGAAGCCG CCTTGGCCCC GCGCAGCGTG AGCCTGAAAG ACAAGGGCCG GTTCCTGGAC
    GGGAGTCCCT ACGCCCACAT GTTTGAGACG CCAGCTGGCG AGAGCACCTT TGCCAACCAC
    GAGTCCTCAG TGGCCGCCGC CGGACACCGC CACCACAACA ACCCCGGCGG CGGCGGCTAC
    ATGCTCAGCA AGTCGCTCTA CCCCGACCGG GTCACGCAAA ACCCTTTCAT CCCCACTTTT
    GGGGACGACC AGTGCTTGCT CCATGGCAGC AAATCCTACT TCTTCAGGCA GCCCACGGTG
    GCGGGGGCGC CGAAAGCCAG GCCAGACTTC CGCGCCCTTG TCACCAACAA GCCGGTGGTC
    TCGGCCCTGC ACGGGGCCGT GCCAGGCCGT TTCCAGAAAG ACATCTGTAT AGGGAACCAG
    TCCAACCCCT GTGTGCCTAA CAACAAAAAC CCCAGGGCTT TCAATGGCTC CAGCAATGGG
    CATGTTTATG AGAAACTTTC TAGTATTGAG TCTGATGTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.