WRN gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_014120071.1	XP_013975546.1	Werner syndrome ATP-dependent helicase isoform X1
XM_014120075.1	XP_013975550.1	Werner syndrome ATP-dependent helicase isoform X5
XM_022404081.1	XP_022259789.1	Werner syndrome ATP-dependent helicase isoform X2
XM_014120072.1	XP_013975547.1	Werner syndrome ATP-dependent helicase isoform X2
XM_014120073.1	XP_013975548.1	Werner syndrome ATP-dependent helicase isoform X3
XM_014120074.1	XP_013975549.1	Werner syndrome ATP-dependent helicase isoform X4
XM_014120071.2	XP_013975546.1	Werner syndrome ATP-dependent helicase isoform X1
XM_014120072.2	XP_013975547.1	Werner syndrome ATP-dependent helicase isoform X3
XM_014120074.2	XP_013975549.1	Werner syndrome ATP-dependent helicase isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe294019	XM_022404081.1 Latest version!	Canis lupus familiaris Werner syndrome RecQ like helicase (WRN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCe40572	XM_014120071.2 Latest version!	Canis lupus familiaris Werner syndrome RecQ like helicase (WRN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCe40573	XM_014120072.2 Latest version!	Canis lupus familiaris Werner syndrome RecQ like helicase (WRN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCe40574	XM_014120073.1 Latest version!	Canis lupus familiaris Werner syndrome, RecQ helicase-like (WRN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCe40575	XM_014120074.2 Latest version!	Canis lupus familiaris Werner syndrome RecQ like helicase (WRN), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe40576	XM_014120075.1 Latest version!	Canis lupus familiaris Werner syndrome, RecQ helicase-like (WRN), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
View Old Accession Versions >>
OCe40572	XM_014120071.1	Canis lupus familiaris Werner syndrome, RecQ helicase-like (WRN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCe40573	XM_014120072.1	Canis lupus familiaris Werner syndrome, RecQ helicase-like (WRN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCe40575	XM_014120074.1	Canis lupus familiaris Werner syndrome, RecQ helicase-like (WRN), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

WRN ( XM_022404081.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022259789.1 Canis lupus familiaris Werner syndrome RecQ like helicase (WRN), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe294019
Clone ID Related Accession (Same CDS sequence)	XM_022404081.1
Accession Version	XM_022404081.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	Werner syndrome ATP-dependent helicase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCTAT TAAAGATGAA TGAAAAAAAA TTGGAAAGAA CTCCACAGCC GCGAAAACTT 
CCTGAATGGA TGTCTATGCA GAATGAGACA TGTACTGCAG TAGAAACAAA GGCATCGATT 
CAGAAGAGTG TTTTTGAAGA CGACCTCTCT TTCTTAGAAT TCACTGGATC TATTGTTTAT 
AGTTATGAAG CTAGTGATTG TTCTTTTCTA TCAGAAGATA TTAGCATGAA TCTGTCTACT 
GGAGGTGTGG TGGGTTTTGA CATGGAATGG CCACCAGTAT ACACTAAAAG GAAACCGAGC 
CGAGTTGCAC TGATTCAGTT ATGTGTGTCA GAGAACAAAT GTTACTTGTT TCATATTTCT 
TCTATGTCAG TTTTTCCTCA GGGGTTAAAA ATGTTGCTTG AAAATGAAGC AATTAAAAAA 
GCAGGTGTAG GAATCAAAGG AGATCAGCGG AAACTTCTAT GTGACTTTGA CATCGATTTG 
AAGAATTTTG TGGAGCTGAC AGATGTTGCC AATGAAAAGG CTGGTCTCAT CATTTATCAG 
AAATTAGAGA TTTTAGGTAA TGCTGTGGAA AGGTTTGCTA TAAATAAAAA AGAGGAGGAA 
ATCCTACCTA TTGATGTGAA GAAACAATTG ACTTCAATCT CTGAGGAGAT GATGGATCTG 
GCCAAGCATC TTCCTGACGC TTTGAAAAAA TTAGAAGATC CACAGAGGGT TTCTCTACTA 
TTAAGTGCTA TTTCAGAAAA TCTGTATTCA TTGAGGAAGA TGATAATTGG TTCTCCTGAG 
GCCGAATTGA GGCACAGCAC TAATGTTCAC TTATTATCAT TGGGAGATCC AACTGCTGAT 
GAAGTACAGC AGAAACAAAT TAGAGAACAT AAATTTTTTC CTAAAGTTAA AGATGAGACT 
TGGGACACAA CATTTGATAA TTTAATTAAA CATGATGAAG GAACCGAACC TCAAAACAAA 
GTGAAACAAA AAGAAGATGG ACTTGAAGAT GGATTAGAAG TTGGCAATAA ATTGAAAACG 
AACACAGAAA GAATTTGTTT GATGTCATTA GATATAACAG AACACGAACT CCAGATTTTG 
GAACAGCAGG CTCAGGAAAA ATTTCTTAAT GATGTCACTT GCAAATCTCC TGAGCATTTA 
TCTTCCAAAG ATAATGAAGA GGATCTATCT TATGTAATTG AAAGCGATGA AGATTTAGAA 
AAGGAAATGA TTAAGTCTTT AGAAAATTTA AATACTGGCA TGGCAGATCC AATTCATTCA 
AAATGCATAA AAATGGAAGG AAATCTGGAT GTTCCTTTTG AAGATGATGA AGATGAGATT 
GAAGCTATTG AAGAAGAAGA TGATAATGTT GAAGACTATT CATTACCAAG ACCTAATGCA 
AGGCAAATTA ATTTCCTCAA GATCTATTTT GGCCATTCCA GTTTTAAACC GGTTCAGTGG 
AAGGTGATTC ATTCTGTATT GGAAGAAAGG AGAGATAATG TTGTTGTGAT GGCAACTGGA 
TATGGAAAGA GTTTGTGCTT CCAGTACCCA CCTGTGTATT CAGGGAGGAT TGGTCTTGTC 
ATCTCTCCTC TTATTTCTTT GATGGAAGAC CAAGTGCTCC AGCTTAAGAT GTCCAATATT 
CCAGCTTGTT TTCTGGGATC AGCACAGTCA AAAAATGTTC TAGAAGATAT TAAATTAGGC 
AAATATCGGA TTGTATACAT AACTCCAGAA TTCTGTTCTG GTAACTTGTT CCTACTCCAG 
CAACTTCAGG CTGATATTGG TATCACACTT ATTGCTGTGG ATGAGGCTCA CTGTATTTCT 
GAATGGGGAC ATGATTTTAG AAGTTCATTC AGGACTTTGG GCTCCCTAAA GGCAATACTC 
CCATCGGTTC CAATTGTTGC ACTTACTGCT ACTGCAAGTT CTTCAATCCG GGAAGACATT 
GTACGCTGCT TAAACCTGAA GAATCCTCAG ATTACCTGTA CTGGTTTTGA TCGACCAAAC 
TTGTATTTAG AAGTTAGGCG AAAAACAGGA AGCATCCTTC AGGATCTTGA CCAATTTCTT 
GTCCAAAAGA CAAGTTCCAT CTGGGAATTT GAAGGTCCAA CTATCATCTA TTGTCCTTCC 
CGAAAAACAA CAGAACAAGT TACAGCTGAA CTTAGGAAAC TGAAGTTAGC CTGTGGAGCA 
TACCATGCAG GCTTGGATAT TAAATCAAGG AGAGAAATTC ATCATAGGTT CATGAGAGAT 
GAAATTCAGT GTGTTGTAGC TACCATAGCT TTTGGAATGG GCATTAATAA AGCTGACATT 
CGCAAAGTCA TTCATTATGG TGCTCCTAAG GAAATGGAAT CATATTACCA GGAGATTGGG 
AGAGCTGGCC GTGATGGACT TCAGAGTTCT TGTCATGTAC TCTGGGCTCC AGCAGACATT 
TGCTTAAATA GGCACCGCCT TAGTGAGATA ACCAGTGAAA AGTTTCGATT ATACAAATTA 
AAGATGATGG CGAAGATGGA AAAATATCTT CATTCCACAA GATGTAGGCG ACAACTCATC 
TTGTCTCATT TTGAAGACAA ACAACTACGA AAGGCCTCCG TGGGTATTAT GGGAACTGAA 
CAATGCTGTG ATAATTGCAG ATCCAGATTG GATCAATGCT CTTCCATTGA TGATTCAGAT 
GACGCATCAC AGGATTTTGG TCCACAAACA TTCCAGCTAT TATCTGCTGT GAAAATCTTA 
GGAGAAAAGT TTGGAATTGG GCTTCCGATT TTATTTCTCC AAGGATCTAA TTCCCAGCGT 
CTTGAAGATA AGTATCGCAG ACACAGTTTA TTTGGCACTG GCAAGGATCA AAAAGAGAGT 
TGGTGGAAGG CTTTGTCCCG TCAGCTCATG GCTGAAGGAT TCTTGGTAGA AGTTCCTGGG 
AGACAAAAAT TTATAAGGAT TTGCACTCTT ACAGAAAAGG GCAGATGTTG GCTTCATGAA 
GCCAATAGAG AATTTCCTAA GAAACTTAAA CTTGAAGCTA ATGAAGAATT GTGTCCGAGG 
GTGTTTCTTC TACCAAGTTC TAAAACTGTA CCACCTGGCG CTGAACAACA TTCCTCTAAT 
CGAGTACCAA TTGAATTAAC TGCAGAGAAG AAGTCTAACT TGGAGAAGAT GTATTCTTAC 
AAAGCATCTG ATAAAATTTC TTCTGGGAGT AACATTCCTG AAAAAAGCAT CATAGTACAC 
TCACCAGGAA AATCTTACAA GTCTTCAGAA CCTGTTATTT CAGCTGAAGA GCAGGAGACT 
CAGACTGTGT TGTATGGCAT ATTGGTGGAA GCCAGGCAGA AACATGCCGG TAAAATGGAT 
GTTCCTCCAG CCATTCTAGC AACAAATAAG ATACTATTGG AAATGGCCAA AATGAGACCT 
ACTACATTTG AAAATATGAA AAGGATCAAT GGTGTGTCTG AAGGCAAAGC TACTATGCTG 
ACCCCTCTGT TGGAAGTCAT CAAACATTTC TGTCAGATAA ATAATGTTCA GTCAGACCTC 
TTTTCAAGTA CAAAACCTTG GGAAGAACCA AGGAACAGTG TGGTGGAGGA AAATGAAACA 
TGTTCCCTTT CACAGTCTGC GGCCATCACG TATTCCTTAT TCCAAGAAAA GAAGATGTCT 
TTGATAAGTT TAACTGAGGA CAGGATTATG CCTCTCACAG CAGTTGGAAT GCACTTAACC 
CAAGCAGTGA AAGCTGGCTG TCCACTTGAT ATGGAGCGAG CAGGCCTGAC TCCAGAGGTT 
CAGAAGATTA TTACTGATGT TATCCGAAAC CCTCCCATCA ACTCAGATAT AAATAAAATT 
AAACTAATTA GAAAGCTGGT TCCTGAAAAC ATTGACACGT ACCTTATCCA CATGGTAATT 
GAGATCCTGG AAAAAGACTC TGACAACAGA CTGTTGTCAG ACGGGCTTTC ATGTGCTTCC 
AACAGAAAGA GATATTTCCC CAATTCTGAA GAGAACTGTT CAAGTTCTAA AAGAAGTAGG 
GAAGAAGTAG GCACTAATAC CAAGGTTGAT GGAAAACATC CTTACGATTT TTCATTGAAA 
GATGAAAGTC ACAAAAATTC CCTAAGACTA ACGTCTTGGA ATCAACCAAC CAGCGATTCA 
GAAACAGATT TATTTACAGA TTCTCACCCA CAGACTTCAT CTATAACCTC CAAGAGAAAA 
TTACCTAAGT GGTTTGCCAA AGACACTGAG ACTTTAGCTG ACACCAGCAG GAAATCAGCA 
GCCAAAGCAC AAAAGAAAGG TCTTTTTAGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022259789.1
CDS	184..4416
Translation	MSLLKMNEKKLERTPQPRKLPEWMSMQNETCTAVETKASIQKSVFEDDLSFLEFTGSIVYSYEASDCSFLSEDISMNLSTGGVVGFDMEWPPVYTKRKPSRVALIQLCVSENKCYLFHISSMSVFPQGLKMLLENEAIKKAGVGIKGDQRKLLCDFDIDLKNFVELTDVANEKAGLIIYQKLEILGNAVERFAINKKEEEILPIDVKKQLTSISEEMMDLAKHLPDALKKLEDPQRVSLLLSAISENLYSLRKMIIGSPEAELRHSTNVHLLSLGDPTADEVQQKQIREHKFFPKVKDETWDTTFDNLIKHDEGTEPQNKVKQKEDGLEDGLEVGNKLKTNTERICLMSLDITEHELQILEQQAQEKFLNDVTCKSPEHLSSKDNEEDLSYVIESDEDLEKEMIKSLENLNTGMADPIHSKCIKMEGNLDVPFEDDEDEIEAIEEEDDNVEDYSLPRPNARQINFLKIYFGHSSFKPVQWKVIHSVLEERRDNVVVMATGYGKSLCFQYPPVYSGRIGLVISPLISLMEDQVLQLKMSNIPACFLGSAQSKNVLEDIKLGKYRIVYITPEFCSGNLFLLQQLQADIGITLIAVDEAHCISEWGHDFRSSFRTLGSLKAILPSVPIVALTATASSSIREDIVRCLNLKNPQITCTGFDRPNLYLEVRRKTGSILQDLDQFLVQKTSSIWEFEGPTIIYCPSRKTTEQVTAELRKLKLACGAYHAGLDIKSRREIHHRFMRDEIQCVVATIAFGMGINKADIRKVIHYGAPKEMESYYQEIGRAGRDGLQSSCHVLWAPADICLNRHRLSEITSEKFRLYKLKMMAKMEKYLHSTRCRRQLILSHFEDKQLRKASVGIMGTEQCCDNCRSRLDQCSSIDDSDDASQDFGPQTFQLLSAVKILGEKFGIGLPILFLQGSNSQRLEDKYRRHSLFGTGKDQKESWWKALSRQLMAEGFLVEVPGRQKFIRICTLTEKGRCWLHEANREFPKKLKLEANEELCPRVFLLPSSKTVPPGAEQHSSNRVPIELTAEKKSNLEKMYSYKASDKISSGSNIPEKSIIVHSPGKSYKSSEPVISAEEQETQTVLYGILVEARQKHAGKMDVPPAILATNKILLEMAKMRPTTFENMKRINGVSEGKATMLTPLLEVIKHFCQINNVQSDLFSSTKPWEEPRNSVVEENETCSLSQSAAITYSLFQEKKMSLISLTEDRIMPLTAVGMHLTQAVKAGCPLDMERAGLTPEVQKIITDVIRNPPINSDINKIKLIRKLVPENIDTYLIHMVIEILEKDSDNRLLSDGLSCASNRKRYFPNSEENCSSSKRSREEVGTNTKVDGKHPYDFSLKDESHKNSLRLTSWNQPTSDSETDLFTDSHPQTSSITSKRKLPKWFAKDTETLADTSRKSAAKAQKKGLFS

Target ORF information:

RefSeq Version	XM_022404081.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris Werner syndrome RecQ like helicase (WRN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022404081.1

ORF Insert Sequence:

ATGAGCCTAT TAAAGATGAA TGAAAAAAAA TTGGAAAGAA CTCCACAGCC GCGAAAACTT 
CCTGAATGGA TGTCTATGCA GAATGAGACA TGTACTGCAG TAGAAACAAA GGCATCGATT 
CAGAAGAGTG TTTTTGAAGA CGACCTCTCT TTCTTAGAAT TCACTGGATC TATTGTTTAT 
AGTTATGAAG CTAGTGATTG TTCTTTTCTA TCAGAAGATA TTAGCATGAA TCTGTCTACT 
GGAGGTGTGG TGGGTTTTGA CATGGAATGG CCACCAGTAT ACACTAAAAG GAAACCGAGC 
CGAGTTGCAC TGATTCAGTT ATGTGTGTCA GAGAACAAAT GTTACTTGTT TCATATTTCT 
TCTATGTCAG TTTTTCCTCA GGGGTTAAAA ATGTTGCTTG AAAATGAAGC AATTAAAAAA 
GCAGGTGTAG GAATCAAAGG AGATCAGCGG AAACTTCTAT GTGACTTTGA CATCGATTTG 
AAGAATTTTG TGGAGCTGAC AGATGTTGCC AATGAAAAGG CTGGTCTCAT CATTTATCAG 
AAATTAGAGA TTTTAGGTAA TGCTGTGGAA AGGTTTGCTA TAAATAAAAA AGAGGAGGAA 
ATCCTACCTA TTGATGTGAA GAAACAATTG ACTTCAATCT CTGAGGAGAT GATGGATCTG 
GCCAAGCATC TTCCTGACGC TTTGAAAAAA TTAGAAGATC CACAGAGGGT TTCTCTACTA 
TTAAGTGCTA TTTCAGAAAA TCTGTATTCA TTGAGGAAGA TGATAATTGG TTCTCCTGAG 
GCCGAATTGA GGCACAGCAC TAATGTTCAC TTATTATCAT TGGGAGATCC AACTGCTGAT 
GAAGTACAGC AGAAACAAAT TAGAGAACAT AAATTTTTTC CTAAAGTTAA AGATGAGACT 
TGGGACACAA CATTTGATAA TTTAATTAAA CATGATGAAG GAACCGAACC TCAAAACAAA 
GTGAAACAAA AAGAAGATGG ACTTGAAGAT GGATTAGAAG TTGGCAATAA ATTGAAAACG 
AACACAGAAA GAATTTGTTT GATGTCATTA GATATAACAG AACACGAACT CCAGATTTTG 
GAACAGCAGG CTCAGGAAAA ATTTCTTAAT GATGTCACTT GCAAATCTCC TGAGCATTTA 
TCTTCCAAAG ATAATGAAGA GGATCTATCT TATGTAATTG AAAGCGATGA AGATTTAGAA 
AAGGAAATGA TTAAGTCTTT AGAAAATTTA AATACTGGCA TGGCAGATCC AATTCATTCA 
AAATGCATAA AAATGGAAGG AAATCTGGAT GTTCCTTTTG AAGATGATGA AGATGAGATT 
GAAGCTATTG AAGAAGAAGA TGATAATGTT GAAGACTATT CATTACCAAG ACCTAATGCA 
AGGCAAATTA ATTTCCTCAA GATCTATTTT GGCCATTCCA GTTTTAAACC GGTTCAGTGG 
AAGGTGATTC ATTCTGTATT GGAAGAAAGG AGAGATAATG TTGTTGTGAT GGCAACTGGA 
TATGGAAAGA GTTTGTGCTT CCAGTACCCA CCTGTGTATT CAGGGAGGAT TGGTCTTGTC 
ATCTCTCCTC TTATTTCTTT GATGGAAGAC CAAGTGCTCC AGCTTAAGAT GTCCAATATT 
CCAGCTTGTT TTCTGGGATC AGCACAGTCA AAAAATGTTC TAGAAGATAT TAAATTAGGC 
AAATATCGGA TTGTATACAT AACTCCAGAA TTCTGTTCTG GTAACTTGTT CCTACTCCAG 
CAACTTCAGG CTGATATTGG TATCACACTT ATTGCTGTGG ATGAGGCTCA CTGTATTTCT 
GAATGGGGAC ATGATTTTAG AAGTTCATTC AGGACTTTGG GCTCCCTAAA GGCAATACTC 
CCATCGGTTC CAATTGTTGC ACTTACTGCT ACTGCAAGTT CTTCAATCCG GGAAGACATT 
GTACGCTGCT TAAACCTGAA GAATCCTCAG ATTACCTGTA CTGGTTTTGA TCGACCAAAC 
TTGTATTTAG AAGTTAGGCG AAAAACAGGA AGCATCCTTC AGGATCTTGA CCAATTTCTT 
GTCCAAAAGA CAAGTTCCAT CTGGGAATTT GAAGGTCCAA CTATCATCTA TTGTCCTTCC 
CGAAAAACAA CAGAACAAGT TACAGCTGAA CTTAGGAAAC TGAAGTTAGC CTGTGGAGCA 
TACCATGCAG GCTTGGATAT TAAATCAAGG AGAGAAATTC ATCATAGGTT CATGAGAGAT 
GAAATTCAGT GTGTTGTAGC TACCATAGCT TTTGGAATGG GCATTAATAA AGCTGACATT 
CGCAAAGTCA TTCATTATGG TGCTCCTAAG GAAATGGAAT CATATTACCA GGAGATTGGG 
AGAGCTGGCC GTGATGGACT TCAGAGTTCT TGTCATGTAC TCTGGGCTCC AGCAGACATT 
TGCTTAAATA GGCACCGCCT TAGTGAGATA ACCAGTGAAA AGTTTCGATT ATACAAATTA 
AAGATGATGG CGAAGATGGA AAAATATCTT CATTCCACAA GATGTAGGCG ACAACTCATC 
TTGTCTCATT TTGAAGACAA ACAACTACGA AAGGCCTCCG TGGGTATTAT GGGAACTGAA 
CAATGCTGTG ATAATTGCAG ATCCAGATTG GATCAATGCT CTTCCATTGA TGATTCAGAT 
GACGCATCAC AGGATTTTGG TCCACAAACA TTCCAGCTAT TATCTGCTGT GAAAATCTTA 
GGAGAAAAGT TTGGAATTGG GCTTCCGATT TTATTTCTCC AAGGATCTAA TTCCCAGCGT 
CTTGAAGATA AGTATCGCAG ACACAGTTTA TTTGGCACTG GCAAGGATCA AAAAGAGAGT 
TGGTGGAAGG CTTTGTCCCG TCAGCTCATG GCTGAAGGAT TCTTGGTAGA AGTTCCTGGG 
AGACAAAAAT TTATAAGGAT TTGCACTCTT ACAGAAAAGG GCAGATGTTG GCTTCATGAA 
GCCAATAGAG AATTTCCTAA GAAACTTAAA CTTGAAGCTA ATGAAGAATT GTGTCCGAGG 
GTGTTTCTTC TACCAAGTTC TAAAACTGTA CCACCTGGCG CTGAACAACA TTCCTCTAAT 
CGAGTACCAA TTGAATTAAC TGCAGAGAAG AAGTCTAACT TGGAGAAGAT GTATTCTTAC 
AAAGCATCTG ATAAAATTTC TTCTGGGAGT AACATTCCTG AAAAAAGCAT CATAGTACAC 
TCACCAGGAA AATCTTACAA GTCTTCAGAA CCTGTTATTT CAGCTGAAGA GCAGGAGACT 
CAGACTGTGT TGTATGGCAT ATTGGTGGAA GCCAGGCAGA AACATGCCGG TAAAATGGAT 
GTTCCTCCAG CCATTCTAGC AACAAATAAG ATACTATTGG AAATGGCCAA AATGAGACCT 
ACTACATTTG AAAATATGAA AAGGATCAAT GGTGTGTCTG AAGGCAAAGC TACTATGCTG 
ACCCCTCTGT TGGAAGTCAT CAAACATTTC TGTCAGATAA ATAATGTTCA GTCAGACCTC 
TTTTCAAGTA CAAAACCTTG GGAAGAACCA AGGAACAGTG TGGTGGAGGA AAATGAAACA 
TGTTCCCTTT CACAGTCTGC GGCCATCACG TATTCCTTAT TCCAAGAAAA GAAGATGTCT 
TTGATAAGTT TAACTGAGGA CAGGATTATG CCTCTCACAG CAGTTGGAAT GCACTTAACC 
CAAGCAGTGA AAGCTGGCTG TCCACTTGAT ATGGAGCGAG CAGGCCTGAC TCCAGAGGTT 
CAGAAGATTA TTACTGATGT TATCCGAAAC CCTCCCATCA ACTCAGATAT AAATAAAATT 
AAACTAATTA GAAAGCTGGT TCCTGAAAAC ATTGACACGT ACCTTATCCA CATGGTAATT 
GAGATCCTGG AAAAAGACTC TGACAACAGA CTGTTGTCAG ACGGGCTTTC ATGTGCTTCC 
AACAGAAAGA GATATTTCCC CAATTCTGAA GAGAACTGTT CAAGTTCTAA AAGAAGTAGG 
GAAGAAGTAG GCACTAATAC CAAGGTTGAT GGAAAACATC CTTACGATTT TTCATTGAAA 
GATGAAAGTC ACAAAAATTC CCTAAGACTA ACGTCTTGGA ATCAACCAAC CAGCGATTCA 
GAAACAGATT TATTTACAGA TTCTCACCCA CAGACTTCAT CTATAACCTC CAAGAGAAAA 
TTACCTAAGT GGTTTGCCAA AGACACTGAG ACTTTAGCTG ACACCAGCAG GAAATCAGCA 
GCCAAAGCAC AAAAGAAAGG TCTTTTTAGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WRN ( XM_014120071.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013975546.1 Canis lupus familiaris Werner syndrome, RecQ helicase-like (WRN), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe40572
Clone ID Related Accession (Same CDS sequence)	XM_014120071.1 , XM_014120071.2
Accession Version	XM_014120071.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	Werner syndrome ATP-dependent helicase isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726087.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCTAT TAAAGATGAA TGAAAAAAAA TTGGAAAGAA CTCCACAGCC GCGAAAACTT 
CCTGAATGGA TGTCTATGCA GAATGAGACA TGTACTGCAG TAGAAACAAA GGCATCGATT 
CAGAAGAGTG TTTTTGAAGA CGACCTCTCT TTCTTAGAAT TCACTGGATC TATTGTTTAT 
AGTTATGAAG CTAGTGATTG TTCTTTTCTA TCAGAAGATA TTAGCATGAA TCTGTCTACT 
GGAGGTGTGG TGGGTTTTGA CATGGAATGG CCACCAGTAT ACACTAAAAG GAAACCGAGC 
CGAGTTGCAC TGATTCAGTT ATGTGTGTCA GAGAACAAAT GTTACTTGTT TCATATTTCT 
TCTATGTCAG TTTTTCCTCA GGGGTTAAAA ATGTTGCTTG AAAATGAAGC AATTAAAAAA 
GCAGGTGTAG GAATCAAAGG AGATCAGCGG AAACTTCTAT GTGACTTTGA CATCGATTTG 
AAGAATTTTG TGGAGCTGAC AGATGTTGCC AATGAAAAGC TGAAATGTAC AGAGACTTGG 
AGCCTCAATG GTTTGGTTAA ACACCTCTTT GGCAAACAGC TTCTGAAAGA CAGATCTATC 
CGTTGTAGCA ATTGGGGTGA TTTTCCTCTC ACTGAGGATC AGAAACTGTA TGCAGCCACT 
GATGCGTATG CTGGTCTCAT CATTTATCAG AAATTAGAGA TTTTAGGTAA TGCTGTGGAA 
AGGTTTGCTA TAAATAAAAA AGAGGAGGAA ATCCTACCTA TTGATGTGAA GAAACAATTG 
ACTTCAATCT CTGAGGAGAT GATGGATCTG GCCAAGCATC TTCCTGACGC TTTGAAAAAA 
TTAGAAGATC CACAGAGGGT TTCTCTACTA TTAAGTGCTA TTTCAGAAAA TCTGTATTCA 
TTGAGGAAGA TGATAATTGG TTCTCCTGAG GCCGAATTGA GGCACAGCAC TAATGTTCAC 
TTATTATCAT TGGGAGATCC AACTGCTGAT GAAGTACAGC AGAAACAAAT TAGAGAACAT 
AAATTTTTTC CTAAAGTTAA AGATGAGACT TGGGACACAA CATTTGATAA TTTAATTAAA 
CATGATGAAG GAACCGAACC TCAAAACAAA GTGAAACAAA AAGAAGATGG ACTTGAAGAT 
GGATTAGAAG TTGGCAATAA ATTGAAAACG AACACAGAAA GAATTTGTTT GATGTCATTA 
GATATAACAG AACACGAACT CCAGATTTTG GAACAGCAGG CTCAGGAAAA ATTTCTTAAT 
GATGTCACTT GCAAATCTCC TGAGCATTTA TCTTCCAAAG ATAATGAAGA GGATCTATCT 
TATGTAATTG AAAGCGATGA AGATTTAGAA AAGGAAATGA TTAAGTCTTT AGAAAATTTA 
AATACTGGCA TGGCAGATCC AATTCATTCA AAATGCATAA AAATGGAAGG AAATCTGGAT 
GTTCCTTTTG AAGATGATGA AGATGAGATT GAAGCTATTG AAGAAGAAGA TGATAATGTT 
GAAGACTATT CATTACCAAG ACCTAATGCA AGGCAAATTA ATTTCCTCAA GATCTATTTT 
GGCCATTCCA GTTTTAAACC GGTTCAGTGG AAGGTGATTC ATTCTGTATT GGAAGAAAGG 
AGAGATAATG TTGTTGTGAT GGCAACTGGA TATGGAAAGA GTTTGTGCTT CCAGTACCCA 
CCTGTGTATT CAGGGAGGAT TGGTCTTGTC ATCTCTCCTC TTATTTCTTT GATGGAAGAC 
CAAGTGCTCC AGCTTAAGAT GTCCAATATT CCAGCTTGTT TTCTGGGATC AGCACAGTCA 
AAAAATGTTC TAGAAGATAT TAAATTAGGC AAATATCGGA TTGTATACAT AACTCCAGAA 
TTCTGTTCTG GTAACTTGTT CCTACTCCAG CAACTTCAGG CTGATATTGG TATCACACTT 
ATTGCTGTGG ATGAGGCTCA CTGTATTTCT GAATGGGGAC ATGATTTTAG AAGTTCATTC 
AGGACTTTGG GCTCCCTAAA GGCAATACTC CCATCGGTTC CAATTGTTGC ACTTACTGCT 
ACTGCAAGTT CTTCAATCCG GGAAGACATT GTACGCTGCT TAAACCTGAA GAATCCTCAG 
ATTACCTGTA CTGGTTTTGA TCGACCAAAC TTGTATTTAG AAGTTAGGCG AAAAACAGGA 
AGCATCCTTC AGGATCTTGA CCAATTTCTT GTCCAAAAGA CAAGTTCCAT CTGGGAATTT 
GAAGGTCCAA CTATCATCTA TTGTCCTTCC CGAAAAACAA CAGAACAAGT TACAGCTGAA 
CTTAGGAAAC TGAAGTTAGC CTGTGGAGCA TACCATGCAG GCTTGGATAT TAAATCAAGG 
AGAGAAATTC ATCATAGGTT CATGAGAGAT GAAATTCAGT GTGTTGTAGC TACCATAGCT 
TTTGGAATGG GCATTAATAA AGCTGACATT CGCAAAGTCA TTCATTATGG TGCTCCTAAG 
GAAATGGAAT CATATTACCA GGAGATTGGG AGAGCTGGCC GTGATGGACT TCAGAGTTCT 
TGTCATGTAC TCTGGGCTCC AGCAGACATT TGCTTAAATA GGCACCGCCT TAGTGAGATA 
ACCAGTGAAA AGTTTCGATT ATACAAATTA AAGATGATGG CGAAGATGGA AAAATATCTT 
CATTCCACAA GATGTAGGCG ACAACTCATC TTGTCTCATT TTGAAGACAA ACAACTACGA 
AAGGCCTCCG TGGGTATTAT GGGAACTGAA CAATGCTGTG ATAATTGCAG ATCCAGATTG 
GATCAATGCT CTTCCATTGA TGATTCAGAT GACGCATCAC AGGATTTTGG TCCACAAACA 
TTCCAGCTAT TATCTGCTGT GAAAATCTTA GGAGAAAAGT TTGGAATTGG GCTTCCGATT 
TTATTTCTCC AAGGATCTAA TTCCCAGCGT CTTGAAGATA AGTATCGCAG ACACAGTTTA 
TTTGGCACTG GCAAGGATCA AAAAGAGAGT TGGTGGAAGG CTTTGTCCCG TCAGCTCATG 
GCTGAAGGAT TCTTGGTAGA AGTTCCTGGG AGACAAAAAT TTATAAGGAT TTGCACTCTT 
ACAGAAAAGG GCAGATGTTG GCTTCATGAA GCCAATAGAG AATTTCCTAA GAAACTTAAA 
CTTGAAGCTA ATGAAGAATT GTGTCCGAGG GTGTTTCTTC TACCAAGTTC TAAAACTGTA 
CCACCTGGCG CTGAACAACA TTCCTCTAAT CGAGTACCAA TTGAATTAAC TGCAGAGAAG 
AAGTCTAACT TGGAGAAGAT GTATTCTTAC AAAGCATCTG ATAAAATTTC TTCTGGGAGT 
AACATTCCTG AAAAAAGCAT CATAGTACAC TCACCAGGAA AATCTTACAA GTCTTCAGAA 
CCTGTTATTT CAGCTGAAGA GCAGGAGACT CAGACTGTGT TGTATGGCAT ATTGGTGGAA 
GCCAGGCAGA AACATGCCGG TAAAATGGAT GTTCCTCCAG CCATTCTAGC AACAAATAAG 
ATACTATTGG AAATGGCCAA AATGAGACCT ACTACATTTG AAAATATGAA AAGGATCAAT 
GGTGTGTCTG AAGGCAAAGC TACTATGCTG ACCCCTCTGT TGGAAGTCAT CAAACATTTC 
TGTCAGATAA ATAATGTTCA GTCAGACCTC TTTTCAAGTA CAAAACCTTG GGAAGAACCA 
AGGAACAGTG TGGTGGAGGA AAATGAAACA TGTTCCCTTT CACAGTCTGC GGCCATCACG 
TATTCCTTAT TCCAAGAAAA GAAGATGTCT TTGATAAGTT TAACTGAGGA CAGGATTATG 
CCTCTCACAG CAGTTGGAAT GCACTTAACC CAAGCAGTGA AAGCTGGCTG TCCACTTGAT 
ATGGAGCGAG CAGGCCTGAC TCCAGAGGTT CAGAAGATTA TTACTGATGT TATCCGAAAC 
CCTCCCATCA ACTCAGATAT AAATAAAATT AAACTAATTA GAAAGCTGGT TCCTGAAAAC 
ATTGACACGT ACCTTATCCA CATGGTAATT GAGATCCTGG AAAAAGACTC TGACAACAGA 
CTGTTGTCAG ACGGGCTTTC ATGTGCTTCC AACAGAAAGA GATATTTCCC CAATTCTGAA 
GAGAACTGTT CAAGTTCTAA AAGAAGTAGG GAAGAAGTAG GCACTAATAC CAAGGTTGAT 
GGAAAACATC CTTACGATTT TTCATTGAAA GATGAAAGTC ACAAAAATTC CCTAAGACTA 
ACGTCTTGGA ATCAACCAAC CAGCGATTCA GAAACAGATT TATTTACAGA TTCTCACCCA 
CAGACTTCAT CTATAACCTC CAAGAGAAAA TTACCTAAGT GGTTTGCCAA AGACACTGAG 
ACTTTAGCTG ACACCAGCAG GAAATCAGCA GCCAAAGCAC AAAAGAAAGG TCTTTTTAGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013975546.1
CDS	167..4549
Translation	MSLLKMNEKKLERTPQPRKLPEWMSMQNETCTAVETKASIQKSVFEDDLSFLEFTGSIVYSYEASDCSFLSEDISMNLSTGGVVGFDMEWPPVYTKRKPSRVALIQLCVSENKCYLFHISSMSVFPQGLKMLLENEAIKKAGVGIKGDQRKLLCDFDIDLKNFVELTDVANEKLKCTETWSLNGLVKHLFGKQLLKDRSIRCSNWGDFPLTEDQKLYAATDAYAGLIIYQKLEILGNAVERFAINKKEEEILPIDVKKQLTSISEEMMDLAKHLPDALKKLEDPQRVSLLLSAISENLYSLRKMIIGSPEAELRHSTNVHLLSLGDPTADEVQQKQIREHKFFPKVKDETWDTTFDNLIKHDEGTEPQNKVKQKEDGLEDGLEVGNKLKTNTERICLMSLDITEHELQILEQQAQEKFLNDVTCKSPEHLSSKDNEEDLSYVIESDEDLEKEMIKSLENLNTGMADPIHSKCIKMEGNLDVPFEDDEDEIEAIEEEDDNVEDYSLPRPNARQINFLKIYFGHSSFKPVQWKVIHSVLEERRDNVVVMATGYGKSLCFQYPPVYSGRIGLVISPLISLMEDQVLQLKMSNIPACFLGSAQSKNVLEDIKLGKYRIVYITPEFCSGNLFLLQQLQADIGITLIAVDEAHCISEWGHDFRSSFRTLGSLKAILPSVPIVALTATASSSIREDIVRCLNLKNPQITCTGFDRPNLYLEVRRKTGSILQDLDQFLVQKTSSIWEFEGPTIIYCPSRKTTEQVTAELRKLKLACGAYHAGLDIKSRREIHHRFMRDEIQCVVATIAFGMGINKADIRKVIHYGAPKEMESYYQEIGRAGRDGLQSSCHVLWAPADICLNRHRLSEITSEKFRLYKLKMMAKMEKYLHSTRCRRQLILSHFEDKQLRKASVGIMGTEQCCDNCRSRLDQCSSIDDSDDASQDFGPQTFQLLSAVKILGEKFGIGLPILFLQGSNSQRLEDKYRRHSLFGTGKDQKESWWKALSRQLMAEGFLVEVPGRQKFIRICTLTEKGRCWLHEANREFPKKLKLEANEELCPRVFLLPSSKTVPPGAEQHSSNRVPIELTAEKKSNLEKMYSYKASDKISSGSNIPEKSIIVHSPGKSYKSSEPVISAEEQETQTVLYGILVEARQKHAGKMDVPPAILATNKILLEMAKMRPTTFENMKRINGVSEGKATMLTPLLEVIKHFCQINNVQSDLFSSTKPWEEPRNSVVEENETCSLSQSAAITYSLFQEKKMSLISLTEDRIMPLTAVGMHLTQAVKAGCPLDMERAGLTPEVQKIITDVIRNPPINSDINKIKLIRKLVPENIDTYLIHMVIEILEKDSDNRLLSDGLSCASNRKRYFPNSEENCSSSKRSREEVGTNTKVDGKHPYDFSLKDESHKNSLRLTSWNQPTSDSETDLFTDSHPQTSSITSKRKLPKWFAKDTETLADTSRKSAAKAQKKGLFS

Target ORF information:

RefSeq Version	XM_014120071.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris Werner syndrome, RecQ helicase-like (WRN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120071.1

ORF Insert Sequence:

ATGAGCCTAT TAAAGATGAA TGAAAAAAAA TTGGAAAGAA CTCCACAGCC GCGAAAACTT 
CCTGAATGGA TGTCTATGCA GAATGAGACA TGTACTGCAG TAGAAACAAA GGCATCGATT 
CAGAAGAGTG TTTTTGAAGA CGACCTCTCT TTCTTAGAAT TCACTGGATC TATTGTTTAT 
AGTTATGAAG CTAGTGATTG TTCTTTTCTA TCAGAAGATA TTAGCATGAA TCTGTCTACT 
GGAGGTGTGG TGGGTTTTGA CATGGAATGG CCACCAGTAT ACACTAAAAG GAAACCGAGC 
CGAGTTGCAC TGATTCAGTT ATGTGTGTCA GAGAACAAAT GTTACTTGTT TCATATTTCT 
TCTATGTCAG TTTTTCCTCA GGGGTTAAAA ATGTTGCTTG AAAATGAAGC AATTAAAAAA 
GCAGGTGTAG GAATCAAAGG AGATCAGCGG AAACTTCTAT GTGACTTTGA CATCGATTTG 
AAGAATTTTG TGGAGCTGAC AGATGTTGCC AATGAAAAGC TGAAATGTAC AGAGACTTGG 
AGCCTCAATG GTTTGGTTAA ACACCTCTTT GGCAAACAGC TTCTGAAAGA CAGATCTATC 
CGTTGTAGCA ATTGGGGTGA TTTTCCTCTC ACTGAGGATC AGAAACTGTA TGCAGCCACT 
GATGCGTATG CTGGTCTCAT CATTTATCAG AAATTAGAGA TTTTAGGTAA TGCTGTGGAA 
AGGTTTGCTA TAAATAAAAA AGAGGAGGAA ATCCTACCTA TTGATGTGAA GAAACAATTG 
ACTTCAATCT CTGAGGAGAT GATGGATCTG GCCAAGCATC TTCCTGACGC TTTGAAAAAA 
TTAGAAGATC CACAGAGGGT TTCTCTACTA TTAAGTGCTA TTTCAGAAAA TCTGTATTCA 
TTGAGGAAGA TGATAATTGG TTCTCCTGAG GCCGAATTGA GGCACAGCAC TAATGTTCAC 
TTATTATCAT TGGGAGATCC AACTGCTGAT GAAGTACAGC AGAAACAAAT TAGAGAACAT 
AAATTTTTTC CTAAAGTTAA AGATGAGACT TGGGACACAA CATTTGATAA TTTAATTAAA 
CATGATGAAG GAACCGAACC TCAAAACAAA GTGAAACAAA AAGAAGATGG ACTTGAAGAT 
GGATTAGAAG TTGGCAATAA ATTGAAAACG AACACAGAAA GAATTTGTTT GATGTCATTA 
GATATAACAG AACACGAACT CCAGATTTTG GAACAGCAGG CTCAGGAAAA ATTTCTTAAT 
GATGTCACTT GCAAATCTCC TGAGCATTTA TCTTCCAAAG ATAATGAAGA GGATCTATCT 
TATGTAATTG AAAGCGATGA AGATTTAGAA AAGGAAATGA TTAAGTCTTT AGAAAATTTA 
AATACTGGCA TGGCAGATCC AATTCATTCA AAATGCATAA AAATGGAAGG AAATCTGGAT 
GTTCCTTTTG AAGATGATGA AGATGAGATT GAAGCTATTG AAGAAGAAGA TGATAATGTT 
GAAGACTATT CATTACCAAG ACCTAATGCA AGGCAAATTA ATTTCCTCAA GATCTATTTT 
GGCCATTCCA GTTTTAAACC GGTTCAGTGG AAGGTGATTC ATTCTGTATT GGAAGAAAGG 
AGAGATAATG TTGTTGTGAT GGCAACTGGA TATGGAAAGA GTTTGTGCTT CCAGTACCCA 
CCTGTGTATT CAGGGAGGAT TGGTCTTGTC ATCTCTCCTC TTATTTCTTT GATGGAAGAC 
CAAGTGCTCC AGCTTAAGAT GTCCAATATT CCAGCTTGTT TTCTGGGATC AGCACAGTCA 
AAAAATGTTC TAGAAGATAT TAAATTAGGC AAATATCGGA TTGTATACAT AACTCCAGAA 
TTCTGTTCTG GTAACTTGTT CCTACTCCAG CAACTTCAGG CTGATATTGG TATCACACTT 
ATTGCTGTGG ATGAGGCTCA CTGTATTTCT GAATGGGGAC ATGATTTTAG AAGTTCATTC 
AGGACTTTGG GCTCCCTAAA GGCAATACTC CCATCGGTTC CAATTGTTGC ACTTACTGCT 
ACTGCAAGTT CTTCAATCCG GGAAGACATT GTACGCTGCT TAAACCTGAA GAATCCTCAG 
ATTACCTGTA CTGGTTTTGA TCGACCAAAC TTGTATTTAG AAGTTAGGCG AAAAACAGGA 
AGCATCCTTC AGGATCTTGA CCAATTTCTT GTCCAAAAGA CAAGTTCCAT CTGGGAATTT 
GAAGGTCCAA CTATCATCTA TTGTCCTTCC CGAAAAACAA CAGAACAAGT TACAGCTGAA 
CTTAGGAAAC TGAAGTTAGC CTGTGGAGCA TACCATGCAG GCTTGGATAT TAAATCAAGG 
AGAGAAATTC ATCATAGGTT CATGAGAGAT GAAATTCAGT GTGTTGTAGC TACCATAGCT 
TTTGGAATGG GCATTAATAA AGCTGACATT CGCAAAGTCA TTCATTATGG TGCTCCTAAG 
GAAATGGAAT CATATTACCA GGAGATTGGG AGAGCTGGCC GTGATGGACT TCAGAGTTCT 
TGTCATGTAC TCTGGGCTCC AGCAGACATT TGCTTAAATA GGCACCGCCT TAGTGAGATA 
ACCAGTGAAA AGTTTCGATT ATACAAATTA AAGATGATGG CGAAGATGGA AAAATATCTT 
CATTCCACAA GATGTAGGCG ACAACTCATC TTGTCTCATT TTGAAGACAA ACAACTACGA 
AAGGCCTCCG TGGGTATTAT GGGAACTGAA CAATGCTGTG ATAATTGCAG ATCCAGATTG 
GATCAATGCT CTTCCATTGA TGATTCAGAT GACGCATCAC AGGATTTTGG TCCACAAACA 
TTCCAGCTAT TATCTGCTGT GAAAATCTTA GGAGAAAAGT TTGGAATTGG GCTTCCGATT 
TTATTTCTCC AAGGATCTAA TTCCCAGCGT CTTGAAGATA AGTATCGCAG ACACAGTTTA 
TTTGGCACTG GCAAGGATCA AAAAGAGAGT TGGTGGAAGG CTTTGTCCCG TCAGCTCATG 
GCTGAAGGAT TCTTGGTAGA AGTTCCTGGG AGACAAAAAT TTATAAGGAT TTGCACTCTT 
ACAGAAAAGG GCAGATGTTG GCTTCATGAA GCCAATAGAG AATTTCCTAA GAAACTTAAA 
CTTGAAGCTA ATGAAGAATT GTGTCCGAGG GTGTTTCTTC TACCAAGTTC TAAAACTGTA 
CCACCTGGCG CTGAACAACA TTCCTCTAAT CGAGTACCAA TTGAATTAAC TGCAGAGAAG 
AAGTCTAACT TGGAGAAGAT GTATTCTTAC AAAGCATCTG ATAAAATTTC TTCTGGGAGT 
AACATTCCTG AAAAAAGCAT CATAGTACAC TCACCAGGAA AATCTTACAA GTCTTCAGAA 
CCTGTTATTT CAGCTGAAGA GCAGGAGACT CAGACTGTGT TGTATGGCAT ATTGGTGGAA 
GCCAGGCAGA AACATGCCGG TAAAATGGAT GTTCCTCCAG CCATTCTAGC AACAAATAAG 
ATACTATTGG AAATGGCCAA AATGAGACCT ACTACATTTG AAAATATGAA AAGGATCAAT 
GGTGTGTCTG AAGGCAAAGC TACTATGCTG ACCCCTCTGT TGGAAGTCAT CAAACATTTC 
TGTCAGATAA ATAATGTTCA GTCAGACCTC TTTTCAAGTA CAAAACCTTG GGAAGAACCA 
AGGAACAGTG TGGTGGAGGA AAATGAAACA TGTTCCCTTT CACAGTCTGC GGCCATCACG 
TATTCCTTAT TCCAAGAAAA GAAGATGTCT TTGATAAGTT TAACTGAGGA CAGGATTATG 
CCTCTCACAG CAGTTGGAAT GCACTTAACC CAAGCAGTGA AAGCTGGCTG TCCACTTGAT 
ATGGAGCGAG CAGGCCTGAC TCCAGAGGTT CAGAAGATTA TTACTGATGT TATCCGAAAC 
CCTCCCATCA ACTCAGATAT AAATAAAATT AAACTAATTA GAAAGCTGGT TCCTGAAAAC 
ATTGACACGT ACCTTATCCA CATGGTAATT GAGATCCTGG AAAAAGACTC TGACAACAGA 
CTGTTGTCAG ACGGGCTTTC ATGTGCTTCC AACAGAAAGA GATATTTCCC CAATTCTGAA 
GAGAACTGTT CAAGTTCTAA AAGAAGTAGG GAAGAAGTAG GCACTAATAC CAAGGTTGAT 
GGAAAACATC CTTACGATTT TTCATTGAAA GATGAAAGTC ACAAAAATTC CCTAAGACTA 
ACGTCTTGGA ATCAACCAAC CAGCGATTCA GAAACAGATT TATTTACAGA TTCTCACCCA 
CAGACTTCAT CTATAACCTC CAAGAGAAAA TTACCTAAGT GGTTTGCCAA AGACACTGAG 
ACTTTAGCTG ACACCAGCAG GAAATCAGCA GCCAAAGCAC AAAAGAAAGG TCTTTTTAGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WRN ( XM_014120071.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013975546.1 Canis lupus familiaris Werner syndrome RecQ like helicase (WRN), transcript variant X1, mRNA.

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CloneID	OCe40572
Clone ID Related Accession (Same CDS sequence)	XM_014120071.1 , XM_014120071.2
Accession Version	XM_014120071.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	Werner syndrome ATP-dependent helicase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014120071.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCTAT TAAAGATGAA TGAAAAAAAA TTGGAAAGAA CTCCACAGCC GCGAAAACTT 
CCTGAATGGA TGTCTATGCA GAATGAGACA TGTACTGCAG TAGAAACAAA GGCATCGATT 
CAGAAGAGTG TTTTTGAAGA CGACCTCTCT TTCTTAGAAT TCACTGGATC TATTGTTTAT 
AGTTATGAAG CTAGTGATTG TTCTTTTCTA TCAGAAGATA TTAGCATGAA TCTGTCTACT 
GGAGGTGTGG TGGGTTTTGA CATGGAATGG CCACCAGTAT ACACTAAAAG GAAACCGAGC 
CGAGTTGCAC TGATTCAGTT ATGTGTGTCA GAGAACAAAT GTTACTTGTT TCATATTTCT 
TCTATGTCAG TTTTTCCTCA GGGGTTAAAA ATGTTGCTTG AAAATGAAGC AATTAAAAAA 
GCAGGTGTAG GAATCAAAGG AGATCAGCGG AAACTTCTAT GTGACTTTGA CATCGATTTG 
AAGAATTTTG TGGAGCTGAC AGATGTTGCC AATGAAAAGC TGAAATGTAC AGAGACTTGG 
AGCCTCAATG GTTTGGTTAA ACACCTCTTT GGCAAACAGC TTCTGAAAGA CAGATCTATC 
CGTTGTAGCA ATTGGGGTGA TTTTCCTCTC ACTGAGGATC AGAAACTGTA TGCAGCCACT 
GATGCGTATG CTGGTCTCAT CATTTATCAG AAATTAGAGA TTTTAGGTAA TGCTGTGGAA 
AGGTTTGCTA TAAATAAAAA AGAGGAGGAA ATCCTACCTA TTGATGTGAA GAAACAATTG 
ACTTCAATCT CTGAGGAGAT GATGGATCTG GCCAAGCATC TTCCTGACGC TTTGAAAAAA 
TTAGAAGATC CACAGAGGGT TTCTCTACTA TTAAGTGCTA TTTCAGAAAA TCTGTATTCA 
TTGAGGAAGA TGATAATTGG TTCTCCTGAG GCCGAATTGA GGCACAGCAC TAATGTTCAC 
TTATTATCAT TGGGAGATCC AACTGCTGAT GAAGTACAGC AGAAACAAAT TAGAGAACAT 
AAATTTTTTC CTAAAGTTAA AGATGAGACT TGGGACACAA CATTTGATAA TTTAATTAAA 
CATGATGAAG GAACCGAACC TCAAAACAAA GTGAAACAAA AAGAAGATGG ACTTGAAGAT 
GGATTAGAAG TTGGCAATAA ATTGAAAACG AACACAGAAA GAATTTGTTT GATGTCATTA 
GATATAACAG AACACGAACT CCAGATTTTG GAACAGCAGG CTCAGGAAAA ATTTCTTAAT 
GATGTCACTT GCAAATCTCC TGAGCATTTA TCTTCCAAAG ATAATGAAGA GGATCTATCT 
TATGTAATTG AAAGCGATGA AGATTTAGAA AAGGAAATGA TTAAGTCTTT AGAAAATTTA 
AATACTGGCA TGGCAGATCC AATTCATTCA AAATGCATAA AAATGGAAGG AAATCTGGAT 
GTTCCTTTTG AAGATGATGA AGATGAGATT GAAGCTATTG AAGAAGAAGA TGATAATGTT 
GAAGACTATT CATTACCAAG ACCTAATGCA AGGCAAATTA ATTTCCTCAA GATCTATTTT 
GGCCATTCCA GTTTTAAACC GGTTCAGTGG AAGGTGATTC ATTCTGTATT GGAAGAAAGG 
AGAGATAATG TTGTTGTGAT GGCAACTGGA TATGGAAAGA GTTTGTGCTT CCAGTACCCA 
CCTGTGTATT CAGGGAGGAT TGGTCTTGTC ATCTCTCCTC TTATTTCTTT GATGGAAGAC 
CAAGTGCTCC AGCTTAAGAT GTCCAATATT CCAGCTTGTT TTCTGGGATC AGCACAGTCA 
AAAAATGTTC TAGAAGATAT TAAATTAGGC AAATATCGGA TTGTATACAT AACTCCAGAA 
TTCTGTTCTG GTAACTTGTT CCTACTCCAG CAACTTCAGG CTGATATTGG TATCACACTT 
ATTGCTGTGG ATGAGGCTCA CTGTATTTCT GAATGGGGAC ATGATTTTAG AAGTTCATTC 
AGGACTTTGG GCTCCCTAAA GGCAATACTC CCATCGGTTC CAATTGTTGC ACTTACTGCT 
ACTGCAAGTT CTTCAATCCG GGAAGACATT GTACGCTGCT TAAACCTGAA GAATCCTCAG 
ATTACCTGTA CTGGTTTTGA TCGACCAAAC TTGTATTTAG AAGTTAGGCG AAAAACAGGA 
AGCATCCTTC AGGATCTTGA CCAATTTCTT GTCCAAAAGA CAAGTTCCAT CTGGGAATTT 
GAAGGTCCAA CTATCATCTA TTGTCCTTCC CGAAAAACAA CAGAACAAGT TACAGCTGAA 
CTTAGGAAAC TGAAGTTAGC CTGTGGAGCA TACCATGCAG GCTTGGATAT TAAATCAAGG 
AGAGAAATTC ATCATAGGTT CATGAGAGAT GAAATTCAGT GTGTTGTAGC TACCATAGCT 
TTTGGAATGG GCATTAATAA AGCTGACATT CGCAAAGTCA TTCATTATGG TGCTCCTAAG 
GAAATGGAAT CATATTACCA GGAGATTGGG AGAGCTGGCC GTGATGGACT TCAGAGTTCT 
TGTCATGTAC TCTGGGCTCC AGCAGACATT TGCTTAAATA GGCACCGCCT TAGTGAGATA 
ACCAGTGAAA AGTTTCGATT ATACAAATTA AAGATGATGG CGAAGATGGA AAAATATCTT 
CATTCCACAA GATGTAGGCG ACAACTCATC TTGTCTCATT TTGAAGACAA ACAACTACGA 
AAGGCCTCCG TGGGTATTAT GGGAACTGAA CAATGCTGTG ATAATTGCAG ATCCAGATTG 
GATCAATGCT CTTCCATTGA TGATTCAGAT GACGCATCAC AGGATTTTGG TCCACAAACA 
TTCCAGCTAT TATCTGCTGT GAAAATCTTA GGAGAAAAGT TTGGAATTGG GCTTCCGATT 
TTATTTCTCC AAGGATCTAA TTCCCAGCGT CTTGAAGATA AGTATCGCAG ACACAGTTTA 
TTTGGCACTG GCAAGGATCA AAAAGAGAGT TGGTGGAAGG CTTTGTCCCG TCAGCTCATG 
GCTGAAGGAT TCTTGGTAGA AGTTCCTGGG AGACAAAAAT TTATAAGGAT TTGCACTCTT 
ACAGAAAAGG GCAGATGTTG GCTTCATGAA GCCAATAGAG AATTTCCTAA GAAACTTAAA 
CTTGAAGCTA ATGAAGAATT GTGTCCGAGG GTGTTTCTTC TACCAAGTTC TAAAACTGTA 
CCACCTGGCG CTGAACAACA TTCCTCTAAT CGAGTACCAA TTGAATTAAC TGCAGAGAAG 
AAGTCTAACT TGGAGAAGAT GTATTCTTAC AAAGCATCTG ATAAAATTTC TTCTGGGAGT 
AACATTCCTG AAAAAAGCAT CATAGTACAC TCACCAGGAA AATCTTACAA GTCTTCAGAA 
CCTGTTATTT CAGCTGAAGA GCAGGAGACT CAGACTGTGT TGTATGGCAT ATTGGTGGAA 
GCCAGGCAGA AACATGCCGG TAAAATGGAT GTTCCTCCAG CCATTCTAGC AACAAATAAG 
ATACTATTGG AAATGGCCAA AATGAGACCT ACTACATTTG AAAATATGAA AAGGATCAAT 
GGTGTGTCTG AAGGCAAAGC TACTATGCTG ACCCCTCTGT TGGAAGTCAT CAAACATTTC 
TGTCAGATAA ATAATGTTCA GTCAGACCTC TTTTCAAGTA CAAAACCTTG GGAAGAACCA 
AGGAACAGTG TGGTGGAGGA AAATGAAACA TGTTCCCTTT CACAGTCTGC GGCCATCACG 
TATTCCTTAT TCCAAGAAAA GAAGATGTCT TTGATAAGTT TAACTGAGGA CAGGATTATG 
CCTCTCACAG CAGTTGGAAT GCACTTAACC CAAGCAGTGA AAGCTGGCTG TCCACTTGAT 
ATGGAGCGAG CAGGCCTGAC TCCAGAGGTT CAGAAGATTA TTACTGATGT TATCCGAAAC 
CCTCCCATCA ACTCAGATAT AAATAAAATT AAACTAATTA GAAAGCTGGT TCCTGAAAAC 
ATTGACACGT ACCTTATCCA CATGGTAATT GAGATCCTGG AAAAAGACTC TGACAACAGA 
CTGTTGTCAG ACGGGCTTTC ATGTGCTTCC AACAGAAAGA GATATTTCCC CAATTCTGAA 
GAGAACTGTT CAAGTTCTAA AAGAAGTAGG GAAGAAGTAG GCACTAATAC CAAGGTTGAT 
GGAAAACATC CTTACGATTT TTCATTGAAA GATGAAAGTC ACAAAAATTC CCTAAGACTA 
ACGTCTTGGA ATCAACCAAC CAGCGATTCA GAAACAGATT TATTTACAGA TTCTCACCCA 
CAGACTTCAT CTATAACCTC CAAGAGAAAA TTACCTAAGT GGTTTGCCAA AGACACTGAG 
ACTTTAGCTG ACACCAGCAG GAAATCAGCA GCCAAAGCAC AAAAGAAAGG TCTTTTTAGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013975546.1
CDS	184..4566
Translation	MSLLKMNEKKLERTPQPRKLPEWMSMQNETCTAVETKASIQKSVFEDDLSFLEFTGSIVYSYEASDCSFLSEDISMNLSTGGVVGFDMEWPPVYTKRKPSRVALIQLCVSENKCYLFHISSMSVFPQGLKMLLENEAIKKAGVGIKGDQRKLLCDFDIDLKNFVELTDVANEKLKCTETWSLNGLVKHLFGKQLLKDRSIRCSNWGDFPLTEDQKLYAATDAYAGLIIYQKLEILGNAVERFAINKKEEEILPIDVKKQLTSISEEMMDLAKHLPDALKKLEDPQRVSLLLSAISENLYSLRKMIIGSPEAELRHSTNVHLLSLGDPTADEVQQKQIREHKFFPKVKDETWDTTFDNLIKHDEGTEPQNKVKQKEDGLEDGLEVGNKLKTNTERICLMSLDITEHELQILEQQAQEKFLNDVTCKSPEHLSSKDNEEDLSYVIESDEDLEKEMIKSLENLNTGMADPIHSKCIKMEGNLDVPFEDDEDEIEAIEEEDDNVEDYSLPRPNARQINFLKIYFGHSSFKPVQWKVIHSVLEERRDNVVVMATGYGKSLCFQYPPVYSGRIGLVISPLISLMEDQVLQLKMSNIPACFLGSAQSKNVLEDIKLGKYRIVYITPEFCSGNLFLLQQLQADIGITLIAVDEAHCISEWGHDFRSSFRTLGSLKAILPSVPIVALTATASSSIREDIVRCLNLKNPQITCTGFDRPNLYLEVRRKTGSILQDLDQFLVQKTSSIWEFEGPTIIYCPSRKTTEQVTAELRKLKLACGAYHAGLDIKSRREIHHRFMRDEIQCVVATIAFGMGINKADIRKVIHYGAPKEMESYYQEIGRAGRDGLQSSCHVLWAPADICLNRHRLSEITSEKFRLYKLKMMAKMEKYLHSTRCRRQLILSHFEDKQLRKASVGIMGTEQCCDNCRSRLDQCSSIDDSDDASQDFGPQTFQLLSAVKILGEKFGIGLPILFLQGSNSQRLEDKYRRHSLFGTGKDQKESWWKALSRQLMAEGFLVEVPGRQKFIRICTLTEKGRCWLHEANREFPKKLKLEANEELCPRVFLLPSSKTVPPGAEQHSSNRVPIELTAEKKSNLEKMYSYKASDKISSGSNIPEKSIIVHSPGKSYKSSEPVISAEEQETQTVLYGILVEARQKHAGKMDVPPAILATNKILLEMAKMRPTTFENMKRINGVSEGKATMLTPLLEVIKHFCQINNVQSDLFSSTKPWEEPRNSVVEENETCSLSQSAAITYSLFQEKKMSLISLTEDRIMPLTAVGMHLTQAVKAGCPLDMERAGLTPEVQKIITDVIRNPPINSDINKIKLIRKLVPENIDTYLIHMVIEILEKDSDNRLLSDGLSCASNRKRYFPNSEENCSSSKRSREEVGTNTKVDGKHPYDFSLKDESHKNSLRLTSWNQPTSDSETDLFTDSHPQTSSITSKRKLPKWFAKDTETLADTSRKSAAKAQKKGLFS

Target ORF information:

RefSeq Version	XM_014120071.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris Werner syndrome RecQ like helicase (WRN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120071.2

ORF Insert Sequence:

ATGAGCCTAT TAAAGATGAA TGAAAAAAAA TTGGAAAGAA CTCCACAGCC GCGAAAACTT 
CCTGAATGGA TGTCTATGCA GAATGAGACA TGTACTGCAG TAGAAACAAA GGCATCGATT 
CAGAAGAGTG TTTTTGAAGA CGACCTCTCT TTCTTAGAAT TCACTGGATC TATTGTTTAT 
AGTTATGAAG CTAGTGATTG TTCTTTTCTA TCAGAAGATA TTAGCATGAA TCTGTCTACT 
GGAGGTGTGG TGGGTTTTGA CATGGAATGG CCACCAGTAT ACACTAAAAG GAAACCGAGC 
CGAGTTGCAC TGATTCAGTT ATGTGTGTCA GAGAACAAAT GTTACTTGTT TCATATTTCT 
TCTATGTCAG TTTTTCCTCA GGGGTTAAAA ATGTTGCTTG AAAATGAAGC AATTAAAAAA 
GCAGGTGTAG GAATCAAAGG AGATCAGCGG AAACTTCTAT GTGACTTTGA CATCGATTTG 
AAGAATTTTG TGGAGCTGAC AGATGTTGCC AATGAAAAGC TGAAATGTAC AGAGACTTGG 
AGCCTCAATG GTTTGGTTAA ACACCTCTTT GGCAAACAGC TTCTGAAAGA CAGATCTATC 
CGTTGTAGCA ATTGGGGTGA TTTTCCTCTC ACTGAGGATC AGAAACTGTA TGCAGCCACT 
GATGCGTATG CTGGTCTCAT CATTTATCAG AAATTAGAGA TTTTAGGTAA TGCTGTGGAA 
AGGTTTGCTA TAAATAAAAA AGAGGAGGAA ATCCTACCTA TTGATGTGAA GAAACAATTG 
ACTTCAATCT CTGAGGAGAT GATGGATCTG GCCAAGCATC TTCCTGACGC TTTGAAAAAA 
TTAGAAGATC CACAGAGGGT TTCTCTACTA TTAAGTGCTA TTTCAGAAAA TCTGTATTCA 
TTGAGGAAGA TGATAATTGG TTCTCCTGAG GCCGAATTGA GGCACAGCAC TAATGTTCAC 
TTATTATCAT TGGGAGATCC AACTGCTGAT GAAGTACAGC AGAAACAAAT TAGAGAACAT 
AAATTTTTTC CTAAAGTTAA AGATGAGACT TGGGACACAA CATTTGATAA TTTAATTAAA 
CATGATGAAG GAACCGAACC TCAAAACAAA GTGAAACAAA AAGAAGATGG ACTTGAAGAT 
GGATTAGAAG TTGGCAATAA ATTGAAAACG AACACAGAAA GAATTTGTTT GATGTCATTA 
GATATAACAG AACACGAACT CCAGATTTTG GAACAGCAGG CTCAGGAAAA ATTTCTTAAT 
GATGTCACTT GCAAATCTCC TGAGCATTTA TCTTCCAAAG ATAATGAAGA GGATCTATCT 
TATGTAATTG AAAGCGATGA AGATTTAGAA AAGGAAATGA TTAAGTCTTT AGAAAATTTA 
AATACTGGCA TGGCAGATCC AATTCATTCA AAATGCATAA AAATGGAAGG AAATCTGGAT 
GTTCCTTTTG AAGATGATGA AGATGAGATT GAAGCTATTG AAGAAGAAGA TGATAATGTT 
GAAGACTATT CATTACCAAG ACCTAATGCA AGGCAAATTA ATTTCCTCAA GATCTATTTT 
GGCCATTCCA GTTTTAAACC GGTTCAGTGG AAGGTGATTC ATTCTGTATT GGAAGAAAGG 
AGAGATAATG TTGTTGTGAT GGCAACTGGA TATGGAAAGA GTTTGTGCTT CCAGTACCCA 
CCTGTGTATT CAGGGAGGAT TGGTCTTGTC ATCTCTCCTC TTATTTCTTT GATGGAAGAC 
CAAGTGCTCC AGCTTAAGAT GTCCAATATT CCAGCTTGTT TTCTGGGATC AGCACAGTCA 
AAAAATGTTC TAGAAGATAT TAAATTAGGC AAATATCGGA TTGTATACAT AACTCCAGAA 
TTCTGTTCTG GTAACTTGTT CCTACTCCAG CAACTTCAGG CTGATATTGG TATCACACTT 
ATTGCTGTGG ATGAGGCTCA CTGTATTTCT GAATGGGGAC ATGATTTTAG AAGTTCATTC 
AGGACTTTGG GCTCCCTAAA GGCAATACTC CCATCGGTTC CAATTGTTGC ACTTACTGCT 
ACTGCAAGTT CTTCAATCCG GGAAGACATT GTACGCTGCT TAAACCTGAA GAATCCTCAG 
ATTACCTGTA CTGGTTTTGA TCGACCAAAC TTGTATTTAG AAGTTAGGCG AAAAACAGGA 
AGCATCCTTC AGGATCTTGA CCAATTTCTT GTCCAAAAGA CAAGTTCCAT CTGGGAATTT 
GAAGGTCCAA CTATCATCTA TTGTCCTTCC CGAAAAACAA CAGAACAAGT TACAGCTGAA 
CTTAGGAAAC TGAAGTTAGC CTGTGGAGCA TACCATGCAG GCTTGGATAT TAAATCAAGG 
AGAGAAATTC ATCATAGGTT CATGAGAGAT GAAATTCAGT GTGTTGTAGC TACCATAGCT 
TTTGGAATGG GCATTAATAA AGCTGACATT CGCAAAGTCA TTCATTATGG TGCTCCTAAG 
GAAATGGAAT CATATTACCA GGAGATTGGG AGAGCTGGCC GTGATGGACT TCAGAGTTCT 
TGTCATGTAC TCTGGGCTCC AGCAGACATT TGCTTAAATA GGCACCGCCT TAGTGAGATA 
ACCAGTGAAA AGTTTCGATT ATACAAATTA AAGATGATGG CGAAGATGGA AAAATATCTT 
CATTCCACAA GATGTAGGCG ACAACTCATC TTGTCTCATT TTGAAGACAA ACAACTACGA 
AAGGCCTCCG TGGGTATTAT GGGAACTGAA CAATGCTGTG ATAATTGCAG ATCCAGATTG 
GATCAATGCT CTTCCATTGA TGATTCAGAT GACGCATCAC AGGATTTTGG TCCACAAACA 
TTCCAGCTAT TATCTGCTGT GAAAATCTTA GGAGAAAAGT TTGGAATTGG GCTTCCGATT 
TTATTTCTCC AAGGATCTAA TTCCCAGCGT CTTGAAGATA AGTATCGCAG ACACAGTTTA 
TTTGGCACTG GCAAGGATCA AAAAGAGAGT TGGTGGAAGG CTTTGTCCCG TCAGCTCATG 
GCTGAAGGAT TCTTGGTAGA AGTTCCTGGG AGACAAAAAT TTATAAGGAT TTGCACTCTT 
ACAGAAAAGG GCAGATGTTG GCTTCATGAA GCCAATAGAG AATTTCCTAA GAAACTTAAA 
CTTGAAGCTA ATGAAGAATT GTGTCCGAGG GTGTTTCTTC TACCAAGTTC TAAAACTGTA 
CCACCTGGCG CTGAACAACA TTCCTCTAAT CGAGTACCAA TTGAATTAAC TGCAGAGAAG 
AAGTCTAACT TGGAGAAGAT GTATTCTTAC AAAGCATCTG ATAAAATTTC TTCTGGGAGT 
AACATTCCTG AAAAAAGCAT CATAGTACAC TCACCAGGAA AATCTTACAA GTCTTCAGAA 
CCTGTTATTT CAGCTGAAGA GCAGGAGACT CAGACTGTGT TGTATGGCAT ATTGGTGGAA 
GCCAGGCAGA AACATGCCGG TAAAATGGAT GTTCCTCCAG CCATTCTAGC AACAAATAAG 
ATACTATTGG AAATGGCCAA AATGAGACCT ACTACATTTG AAAATATGAA AAGGATCAAT 
GGTGTGTCTG AAGGCAAAGC TACTATGCTG ACCCCTCTGT TGGAAGTCAT CAAACATTTC 
TGTCAGATAA ATAATGTTCA GTCAGACCTC TTTTCAAGTA CAAAACCTTG GGAAGAACCA 
AGGAACAGTG TGGTGGAGGA AAATGAAACA TGTTCCCTTT CACAGTCTGC GGCCATCACG 
TATTCCTTAT TCCAAGAAAA GAAGATGTCT TTGATAAGTT TAACTGAGGA CAGGATTATG 
CCTCTCACAG CAGTTGGAAT GCACTTAACC CAAGCAGTGA AAGCTGGCTG TCCACTTGAT 
ATGGAGCGAG CAGGCCTGAC TCCAGAGGTT CAGAAGATTA TTACTGATGT TATCCGAAAC 
CCTCCCATCA ACTCAGATAT AAATAAAATT AAACTAATTA GAAAGCTGGT TCCTGAAAAC 
ATTGACACGT ACCTTATCCA CATGGTAATT GAGATCCTGG AAAAAGACTC TGACAACAGA 
CTGTTGTCAG ACGGGCTTTC ATGTGCTTCC AACAGAAAGA GATATTTCCC CAATTCTGAA 
GAGAACTGTT CAAGTTCTAA AAGAAGTAGG GAAGAAGTAG GCACTAATAC CAAGGTTGAT 
GGAAAACATC CTTACGATTT TTCATTGAAA GATGAAAGTC ACAAAAATTC CCTAAGACTA 
ACGTCTTGGA ATCAACCAAC CAGCGATTCA GAAACAGATT TATTTACAGA TTCTCACCCA 
CAGACTTCAT CTATAACCTC CAAGAGAAAA TTACCTAAGT GGTTTGCCAA AGACACTGAG 
ACTTTAGCTG ACACCAGCAG GAAATCAGCA GCCAAAGCAC AAAAGAAAGG TCTTTTTAGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WRN ( XM_014120072.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013975547.1 Canis lupus familiaris Werner syndrome, RecQ helicase-like (WRN), transcript variant X2, mRNA.

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CloneID	OCe40573
Clone ID Related Accession (Same CDS sequence)	XM_014120072.1 , XM_014120072.2
Accession Version	XM_014120072.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	Werner syndrome ATP-dependent helicase isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726087.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGAAA AAAAATTGGA AAGAACTCCA CAGCCGCGAA AACTTCCTGA ATGGATGTCT 
ATGCAGAATG AGACATGTAC TGCAGTAGAA ACAAAGGCAT CGATTCAGAA GAGTGTTTTT 
GAAGACGACC TCTCTTTCTT AGAATTCACT GGATCTATTG TTTATAGTTA TGAAGCTAGT 
GATTGTTCTT TTCTATCAGA AGATATTAGC ATGAATCTGT CTACTGGAGG TGTGGTGGGT 
TTTGACATGG AATGGCCACC AGTATACACT AAAAGGAAAC CGAGCCGAGT TGCACTGATT 
CAGTTATGTG TGTCAGAGAA CAAATGTTAC TTGTTTCATA TTTCTTCTAT GTCAGTTTTT 
CCTCAGGGGT TAAAAATGTT GCTTGAAAAT GAAGCAATTA AAAAAGCAGG TGTAGGAATC 
AAAGGAGATC AGCGGAAACT TCTATGTGAC TTTGACATCG ATTTGAAGAA TTTTGTGGAG 
CTGACAGATG TTGCCAATGA AAAGCTGAAA TGTACAGAGA CTTGGAGCCT CAATGGTTTG 
GTTAAACACC TCTTTGGCAA ACAGCTTCTG AAAGACAGAT CTATCCGTTG TAGCAATTGG 
GGTGATTTTC CTCTCACTGA GGATCAGAAA CTGTATGCAG CCACTGATGC GTATGCTGGT 
CTCATCATTT ATCAGAAATT AGAGATTTTA GGTAATGCTG TGGAAAGGTT TGCTATAAAT 
AAAAAAGAGG AGGAAATCCT ACCTATTGAT GTGAAGAAAC AATTGACTTC AATCTCTGAG 
GAGATGATGG ATCTGGCCAA GCATCTTCCT GACGCTTTGA AAAAATTAGA AGATCCACAG 
AGGGTTTCTC TACTATTAAG TGCTATTTCA GAAAATCTGT ATTCATTGAG GAAGATGATA 
ATTGGTTCTC CTGAGGCCGA ATTGAGGCAC AGCACTAATG TTCACTTATT ATCATTGGGA 
GATCCAACTG CTGATGAAGT ACAGCAGAAA CAAATTAGAG AACATAAATT TTTTCCTAAA 
GTTAAAGATG AGACTTGGGA CACAACATTT GATAATTTAA TTAAACATGA TGAAGGAACC 
GAACCTCAAA ACAAAGTGAA ACAAAAAGAA GATGGACTTG AAGATGGATT AGAAGTTGGC 
AATAAATTGA AAACGAACAC AGAAAGAATT TGTTTGATGT CATTAGATAT AACAGAACAC 
GAACTCCAGA TTTTGGAACA GCAGGCTCAG GAAAAATTTC TTAATGATGT CACTTGCAAA 
TCTCCTGAGC ATTTATCTTC CAAAGATAAT GAAGAGGATC TATCTTATGT AATTGAAAGC 
GATGAAGATT TAGAAAAGGA AATGATTAAG TCTTTAGAAA ATTTAAATAC TGGCATGGCA 
GATCCAATTC ATTCAAAATG CATAAAAATG GAAGGAAATC TGGATGTTCC TTTTGAAGAT 
GATGAAGATG AGATTGAAGC TATTGAAGAA GAAGATGATA ATGTTGAAGA CTATTCATTA 
CCAAGACCTA ATGCAAGGCA AATTAATTTC CTCAAGATCT ATTTTGGCCA TTCCAGTTTT 
AAACCGGTTC AGTGGAAGGT GATTCATTCT GTATTGGAAG AAAGGAGAGA TAATGTTGTT 
GTGATGGCAA CTGGATATGG AAAGAGTTTG TGCTTCCAGT ACCCACCTGT GTATTCAGGG 
AGGATTGGTC TTGTCATCTC TCCTCTTATT TCTTTGATGG AAGACCAAGT GCTCCAGCTT 
AAGATGTCCA ATATTCCAGC TTGTTTTCTG GGATCAGCAC AGTCAAAAAA TGTTCTAGAA 
GATATTAAAT TAGGCAAATA TCGGATTGTA TACATAACTC CAGAATTCTG TTCTGGTAAC 
TTGTTCCTAC TCCAGCAACT TCAGGCTGAT ATTGGTATCA CACTTATTGC TGTGGATGAG 
GCTCACTGTA TTTCTGAATG GGGACATGAT TTTAGAAGTT CATTCAGGAC TTTGGGCTCC 
CTAAAGGCAA TACTCCCATC GGTTCCAATT GTTGCACTTA CTGCTACTGC AAGTTCTTCA 
ATCCGGGAAG ACATTGTACG CTGCTTAAAC CTGAAGAATC CTCAGATTAC CTGTACTGGT 
TTTGATCGAC CAAACTTGTA TTTAGAAGTT AGGCGAAAAA CAGGAAGCAT CCTTCAGGAT 
CTTGACCAAT TTCTTGTCCA AAAGACAAGT TCCATCTGGG AATTTGAAGG TCCAACTATC 
ATCTATTGTC CTTCCCGAAA AACAACAGAA CAAGTTACAG CTGAACTTAG GAAACTGAAG 
TTAGCCTGTG GAGCATACCA TGCAGGCTTG GATATTAAAT CAAGGAGAGA AATTCATCAT 
AGGTTCATGA GAGATGAAAT TCAGTGTGTT GTAGCTACCA TAGCTTTTGG AATGGGCATT 
AATAAAGCTG ACATTCGCAA AGTCATTCAT TATGGTGCTC CTAAGGAAAT GGAATCATAT 
TACCAGGAGA TTGGGAGAGC TGGCCGTGAT GGACTTCAGA GTTCTTGTCA TGTACTCTGG 
GCTCCAGCAG ACATTTGCTT AAATAGGCAC CGCCTTAGTG AGATAACCAG TGAAAAGTTT 
CGATTATACA AATTAAAGAT GATGGCGAAG ATGGAAAAAT ATCTTCATTC CACAAGATGT 
AGGCGACAAC TCATCTTGTC TCATTTTGAA GACAAACAAC TACGAAAGGC CTCCGTGGGT 
ATTATGGGAA CTGAACAATG CTGTGATAAT TGCAGATCCA GATTGGATCA ATGCTCTTCC 
ATTGATGATT CAGATGACGC ATCACAGGAT TTTGGTCCAC AAACATTCCA GCTATTATCT 
GCTGTGAAAA TCTTAGGAGA AAAGTTTGGA ATTGGGCTTC CGATTTTATT TCTCCAAGGA 
TCTAATTCCC AGCGTCTTGA AGATAAGTAT CGCAGACACA GTTTATTTGG CACTGGCAAG 
GATCAAAAAG AGAGTTGGTG GAAGGCTTTG TCCCGTCAGC TCATGGCTGA AGGATTCTTG 
GTAGAAGTTC CTGGGAGACA AAAATTTATA AGGATTTGCA CTCTTACAGA AAAGGGCAGA 
TGTTGGCTTC ATGAAGCCAA TAGAGAATTT CCTAAGAAAC TTAAACTTGA AGCTAATGAA 
GAATTGTGTC CGAGGGTGTT TCTTCTACCA AGTTCTAAAA CTGTACCACC TGGCGCTGAA 
CAACATTCCT CTAATCGAGT ACCAATTGAA TTAACTGCAG AGAAGAAGTC TAACTTGGAG 
AAGATGTATT CTTACAAAGC ATCTGATAAA ATTTCTTCTG GGAGTAACAT TCCTGAAAAA 
AGCATCATAG TACACTCACC AGGAAAATCT TACAAGTCTT CAGAACCTGT TATTTCAGCT 
GAAGAGCAGG AGACTCAGAC TGTGTTGTAT GGCATATTGG TGGAAGCCAG GCAGAAACAT 
GCCGGTAAAA TGGATGTTCC TCCAGCCATT CTAGCAACAA ATAAGATACT ATTGGAAATG 
GCCAAAATGA GACCTACTAC ATTTGAAAAT ATGAAAAGGA TCAATGGTGT GTCTGAAGGC 
AAAGCTACTA TGCTGACCCC TCTGTTGGAA GTCATCAAAC ATTTCTGTCA GATAAATAAT 
GTTCAGTCAG ACCTCTTTTC AAGTACAAAA CCTTGGGAAG AACCAAGGAA CAGTGTGGTG 
GAGGAAAATG AAACATGTTC CCTTTCACAG TCTGCGGCCA TCACGTATTC CTTATTCCAA 
GAAAAGAAGA TGTCTTTGAT AAGTTTAACT GAGGACAGGA TTATGCCTCT CACAGCAGTT 
GGAATGCACT TAACCCAAGC AGTGAAAGCT GGCTGTCCAC TTGATATGGA GCGAGCAGGC 
CTGACTCCAG AGGTTCAGAA GATTATTACT GATGTTATCC GAAACCCTCC CATCAACTCA 
GATATAAATA AAATTAAACT AATTAGAAAG CTGGTTCCTG AAAACATTGA CACGTACCTT 
ATCCACATGG TAATTGAGAT CCTGGAAAAA GACTCTGACA ACAGACTGTT GTCAGACGGG 
CTTTCATGTG CTTCCAACAG AAAGAGATAT TTCCCCAATT CTGAAGAGAA CTGTTCAAGT 
TCTAAAAGAA GTAGGGAAGA AGTAGGCACT AATACCAAGG TTGATGGAAA ACATCCTTAC 
GATTTTTCAT TGAAAGATGA AAGTCACAAA AATTCCCTAA GACTAACGTC TTGGAATCAA 
CCAACCAGCG ATTCAGAAAC AGATTTATTT ACAGATTCTC ACCCACAGAC TTCATCTATA 
ACCTCCAAGA GAAAATTACC TAAGTGGTTT GCCAAAGACA CTGAGACTTT AGCTGACACC 
AGCAGGAAAT CAGCAGCCAA AGCACAAAAG AAAGGTCTTT TTAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013975547.1
CDS	53..4420
Translation	MNEKKLERTPQPRKLPEWMSMQNETCTAVETKASIQKSVFEDDLSFLEFTGSIVYSYEASDCSFLSEDISMNLSTGGVVGFDMEWPPVYTKRKPSRVALIQLCVSENKCYLFHISSMSVFPQGLKMLLENEAIKKAGVGIKGDQRKLLCDFDIDLKNFVELTDVANEKLKCTETWSLNGLVKHLFGKQLLKDRSIRCSNWGDFPLTEDQKLYAATDAYAGLIIYQKLEILGNAVERFAINKKEEEILPIDVKKQLTSISEEMMDLAKHLPDALKKLEDPQRVSLLLSAISENLYSLRKMIIGSPEAELRHSTNVHLLSLGDPTADEVQQKQIREHKFFPKVKDETWDTTFDNLIKHDEGTEPQNKVKQKEDGLEDGLEVGNKLKTNTERICLMSLDITEHELQILEQQAQEKFLNDVTCKSPEHLSSKDNEEDLSYVIESDEDLEKEMIKSLENLNTGMADPIHSKCIKMEGNLDVPFEDDEDEIEAIEEEDDNVEDYSLPRPNARQINFLKIYFGHSSFKPVQWKVIHSVLEERRDNVVVMATGYGKSLCFQYPPVYSGRIGLVISPLISLMEDQVLQLKMSNIPACFLGSAQSKNVLEDIKLGKYRIVYITPEFCSGNLFLLQQLQADIGITLIAVDEAHCISEWGHDFRSSFRTLGSLKAILPSVPIVALTATASSSIREDIVRCLNLKNPQITCTGFDRPNLYLEVRRKTGSILQDLDQFLVQKTSSIWEFEGPTIIYCPSRKTTEQVTAELRKLKLACGAYHAGLDIKSRREIHHRFMRDEIQCVVATIAFGMGINKADIRKVIHYGAPKEMESYYQEIGRAGRDGLQSSCHVLWAPADICLNRHRLSEITSEKFRLYKLKMMAKMEKYLHSTRCRRQLILSHFEDKQLRKASVGIMGTEQCCDNCRSRLDQCSSIDDSDDASQDFGPQTFQLLSAVKILGEKFGIGLPILFLQGSNSQRLEDKYRRHSLFGTGKDQKESWWKALSRQLMAEGFLVEVPGRQKFIRICTLTEKGRCWLHEANREFPKKLKLEANEELCPRVFLLPSSKTVPPGAEQHSSNRVPIELTAEKKSNLEKMYSYKASDKISSGSNIPEKSIIVHSPGKSYKSSEPVISAEEQETQTVLYGILVEARQKHAGKMDVPPAILATNKILLEMAKMRPTTFENMKRINGVSEGKATMLTPLLEVIKHFCQINNVQSDLFSSTKPWEEPRNSVVEENETCSLSQSAAITYSLFQEKKMSLISLTEDRIMPLTAVGMHLTQAVKAGCPLDMERAGLTPEVQKIITDVIRNPPINSDINKIKLIRKLVPENIDTYLIHMVIEILEKDSDNRLLSDGLSCASNRKRYFPNSEENCSSSKRSREEVGTNTKVDGKHPYDFSLKDESHKNSLRLTSWNQPTSDSETDLFTDSHPQTSSITSKRKLPKWFAKDTETLADTSRKSAAKAQKKGLFS

Target ORF information:

RefSeq Version	XM_014120072.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris Werner syndrome, RecQ helicase-like (WRN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120072.1

ORF Insert Sequence:

ATGAATGAAA AAAAATTGGA AAGAACTCCA CAGCCGCGAA AACTTCCTGA ATGGATGTCT 
ATGCAGAATG AGACATGTAC TGCAGTAGAA ACAAAGGCAT CGATTCAGAA GAGTGTTTTT 
GAAGACGACC TCTCTTTCTT AGAATTCACT GGATCTATTG TTTATAGTTA TGAAGCTAGT 
GATTGTTCTT TTCTATCAGA AGATATTAGC ATGAATCTGT CTACTGGAGG TGTGGTGGGT 
TTTGACATGG AATGGCCACC AGTATACACT AAAAGGAAAC CGAGCCGAGT TGCACTGATT 
CAGTTATGTG TGTCAGAGAA CAAATGTTAC TTGTTTCATA TTTCTTCTAT GTCAGTTTTT 
CCTCAGGGGT TAAAAATGTT GCTTGAAAAT GAAGCAATTA AAAAAGCAGG TGTAGGAATC 
AAAGGAGATC AGCGGAAACT TCTATGTGAC TTTGACATCG ATTTGAAGAA TTTTGTGGAG 
CTGACAGATG TTGCCAATGA AAAGCTGAAA TGTACAGAGA CTTGGAGCCT CAATGGTTTG 
GTTAAACACC TCTTTGGCAA ACAGCTTCTG AAAGACAGAT CTATCCGTTG TAGCAATTGG 
GGTGATTTTC CTCTCACTGA GGATCAGAAA CTGTATGCAG CCACTGATGC GTATGCTGGT 
CTCATCATTT ATCAGAAATT AGAGATTTTA GGTAATGCTG TGGAAAGGTT TGCTATAAAT 
AAAAAAGAGG AGGAAATCCT ACCTATTGAT GTGAAGAAAC AATTGACTTC AATCTCTGAG 
GAGATGATGG ATCTGGCCAA GCATCTTCCT GACGCTTTGA AAAAATTAGA AGATCCACAG 
AGGGTTTCTC TACTATTAAG TGCTATTTCA GAAAATCTGT ATTCATTGAG GAAGATGATA 
ATTGGTTCTC CTGAGGCCGA ATTGAGGCAC AGCACTAATG TTCACTTATT ATCATTGGGA 
GATCCAACTG CTGATGAAGT ACAGCAGAAA CAAATTAGAG AACATAAATT TTTTCCTAAA 
GTTAAAGATG AGACTTGGGA CACAACATTT GATAATTTAA TTAAACATGA TGAAGGAACC 
GAACCTCAAA ACAAAGTGAA ACAAAAAGAA GATGGACTTG AAGATGGATT AGAAGTTGGC 
AATAAATTGA AAACGAACAC AGAAAGAATT TGTTTGATGT CATTAGATAT AACAGAACAC 
GAACTCCAGA TTTTGGAACA GCAGGCTCAG GAAAAATTTC TTAATGATGT CACTTGCAAA 
TCTCCTGAGC ATTTATCTTC CAAAGATAAT GAAGAGGATC TATCTTATGT AATTGAAAGC 
GATGAAGATT TAGAAAAGGA AATGATTAAG TCTTTAGAAA ATTTAAATAC TGGCATGGCA 
GATCCAATTC ATTCAAAATG CATAAAAATG GAAGGAAATC TGGATGTTCC TTTTGAAGAT 
GATGAAGATG AGATTGAAGC TATTGAAGAA GAAGATGATA ATGTTGAAGA CTATTCATTA 
CCAAGACCTA ATGCAAGGCA AATTAATTTC CTCAAGATCT ATTTTGGCCA TTCCAGTTTT 
AAACCGGTTC AGTGGAAGGT GATTCATTCT GTATTGGAAG AAAGGAGAGA TAATGTTGTT 
GTGATGGCAA CTGGATATGG AAAGAGTTTG TGCTTCCAGT ACCCACCTGT GTATTCAGGG 
AGGATTGGTC TTGTCATCTC TCCTCTTATT TCTTTGATGG AAGACCAAGT GCTCCAGCTT 
AAGATGTCCA ATATTCCAGC TTGTTTTCTG GGATCAGCAC AGTCAAAAAA TGTTCTAGAA 
GATATTAAAT TAGGCAAATA TCGGATTGTA TACATAACTC CAGAATTCTG TTCTGGTAAC 
TTGTTCCTAC TCCAGCAACT TCAGGCTGAT ATTGGTATCA CACTTATTGC TGTGGATGAG 
GCTCACTGTA TTTCTGAATG GGGACATGAT TTTAGAAGTT CATTCAGGAC TTTGGGCTCC 
CTAAAGGCAA TACTCCCATC GGTTCCAATT GTTGCACTTA CTGCTACTGC AAGTTCTTCA 
ATCCGGGAAG ACATTGTACG CTGCTTAAAC CTGAAGAATC CTCAGATTAC CTGTACTGGT 
TTTGATCGAC CAAACTTGTA TTTAGAAGTT AGGCGAAAAA CAGGAAGCAT CCTTCAGGAT 
CTTGACCAAT TTCTTGTCCA AAAGACAAGT TCCATCTGGG AATTTGAAGG TCCAACTATC 
ATCTATTGTC CTTCCCGAAA AACAACAGAA CAAGTTACAG CTGAACTTAG GAAACTGAAG 
TTAGCCTGTG GAGCATACCA TGCAGGCTTG GATATTAAAT CAAGGAGAGA AATTCATCAT 
AGGTTCATGA GAGATGAAAT TCAGTGTGTT GTAGCTACCA TAGCTTTTGG AATGGGCATT 
AATAAAGCTG ACATTCGCAA AGTCATTCAT TATGGTGCTC CTAAGGAAAT GGAATCATAT 
TACCAGGAGA TTGGGAGAGC TGGCCGTGAT GGACTTCAGA GTTCTTGTCA TGTACTCTGG 
GCTCCAGCAG ACATTTGCTT AAATAGGCAC CGCCTTAGTG AGATAACCAG TGAAAAGTTT 
CGATTATACA AATTAAAGAT GATGGCGAAG ATGGAAAAAT ATCTTCATTC CACAAGATGT 
AGGCGACAAC TCATCTTGTC TCATTTTGAA GACAAACAAC TACGAAAGGC CTCCGTGGGT 
ATTATGGGAA CTGAACAATG CTGTGATAAT TGCAGATCCA GATTGGATCA ATGCTCTTCC 
ATTGATGATT CAGATGACGC ATCACAGGAT TTTGGTCCAC AAACATTCCA GCTATTATCT 
GCTGTGAAAA TCTTAGGAGA AAAGTTTGGA ATTGGGCTTC CGATTTTATT TCTCCAAGGA 
TCTAATTCCC AGCGTCTTGA AGATAAGTAT CGCAGACACA GTTTATTTGG CACTGGCAAG 
GATCAAAAAG AGAGTTGGTG GAAGGCTTTG TCCCGTCAGC TCATGGCTGA AGGATTCTTG 
GTAGAAGTTC CTGGGAGACA AAAATTTATA AGGATTTGCA CTCTTACAGA AAAGGGCAGA 
TGTTGGCTTC ATGAAGCCAA TAGAGAATTT CCTAAGAAAC TTAAACTTGA AGCTAATGAA 
GAATTGTGTC CGAGGGTGTT TCTTCTACCA AGTTCTAAAA CTGTACCACC TGGCGCTGAA 
CAACATTCCT CTAATCGAGT ACCAATTGAA TTAACTGCAG AGAAGAAGTC TAACTTGGAG 
AAGATGTATT CTTACAAAGC ATCTGATAAA ATTTCTTCTG GGAGTAACAT TCCTGAAAAA 
AGCATCATAG TACACTCACC AGGAAAATCT TACAAGTCTT CAGAACCTGT TATTTCAGCT 
GAAGAGCAGG AGACTCAGAC TGTGTTGTAT GGCATATTGG TGGAAGCCAG GCAGAAACAT 
GCCGGTAAAA TGGATGTTCC TCCAGCCATT CTAGCAACAA ATAAGATACT ATTGGAAATG 
GCCAAAATGA GACCTACTAC ATTTGAAAAT ATGAAAAGGA TCAATGGTGT GTCTGAAGGC 
AAAGCTACTA TGCTGACCCC TCTGTTGGAA GTCATCAAAC ATTTCTGTCA GATAAATAAT 
GTTCAGTCAG ACCTCTTTTC AAGTACAAAA CCTTGGGAAG AACCAAGGAA CAGTGTGGTG 
GAGGAAAATG AAACATGTTC CCTTTCACAG TCTGCGGCCA TCACGTATTC CTTATTCCAA 
GAAAAGAAGA TGTCTTTGAT AAGTTTAACT GAGGACAGGA TTATGCCTCT CACAGCAGTT 
GGAATGCACT TAACCCAAGC AGTGAAAGCT GGCTGTCCAC TTGATATGGA GCGAGCAGGC 
CTGACTCCAG AGGTTCAGAA GATTATTACT GATGTTATCC GAAACCCTCC CATCAACTCA 
GATATAAATA AAATTAAACT AATTAGAAAG CTGGTTCCTG AAAACATTGA CACGTACCTT 
ATCCACATGG TAATTGAGAT CCTGGAAAAA GACTCTGACA ACAGACTGTT GTCAGACGGG 
CTTTCATGTG CTTCCAACAG AAAGAGATAT TTCCCCAATT CTGAAGAGAA CTGTTCAAGT 
TCTAAAAGAA GTAGGGAAGA AGTAGGCACT AATACCAAGG TTGATGGAAA ACATCCTTAC 
GATTTTTCAT TGAAAGATGA AAGTCACAAA AATTCCCTAA GACTAACGTC TTGGAATCAA 
CCAACCAGCG ATTCAGAAAC AGATTTATTT ACAGATTCTC ACCCACAGAC TTCATCTATA 
ACCTCCAAGA GAAAATTACC TAAGTGGTTT GCCAAAGACA CTGAGACTTT AGCTGACACC 
AGCAGGAAAT CAGCAGCCAA AGCACAAAAG AAAGGTCTTT TTAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WRN ( XM_014120072.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013975547.1 Canis lupus familiaris Werner syndrome RecQ like helicase (WRN), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe40573
Clone ID Related Accession (Same CDS sequence)	XM_014120072.1 , XM_014120072.2
Accession Version	XM_014120072.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	Werner syndrome ATP-dependent helicase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014120072.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGAAA AAAAATTGGA AAGAACTCCA CAGCCGCGAA AACTTCCTGA ATGGATGTCT 
ATGCAGAATG AGACATGTAC TGCAGTAGAA ACAAAGGCAT CGATTCAGAA GAGTGTTTTT 
GAAGACGACC TCTCTTTCTT AGAATTCACT GGATCTATTG TTTATAGTTA TGAAGCTAGT 
GATTGTTCTT TTCTATCAGA AGATATTAGC ATGAATCTGT CTACTGGAGG TGTGGTGGGT 
TTTGACATGG AATGGCCACC AGTATACACT AAAAGGAAAC CGAGCCGAGT TGCACTGATT 
CAGTTATGTG TGTCAGAGAA CAAATGTTAC TTGTTTCATA TTTCTTCTAT GTCAGTTTTT 
CCTCAGGGGT TAAAAATGTT GCTTGAAAAT GAAGCAATTA AAAAAGCAGG TGTAGGAATC 
AAAGGAGATC AGCGGAAACT TCTATGTGAC TTTGACATCG ATTTGAAGAA TTTTGTGGAG 
CTGACAGATG TTGCCAATGA AAAGCTGAAA TGTACAGAGA CTTGGAGCCT CAATGGTTTG 
GTTAAACACC TCTTTGGCAA ACAGCTTCTG AAAGACAGAT CTATCCGTTG TAGCAATTGG 
GGTGATTTTC CTCTCACTGA GGATCAGAAA CTGTATGCAG CCACTGATGC GTATGCTGGT 
CTCATCATTT ATCAGAAATT AGAGATTTTA GGTAATGCTG TGGAAAGGTT TGCTATAAAT 
AAAAAAGAGG AGGAAATCCT ACCTATTGAT GTGAAGAAAC AATTGACTTC AATCTCTGAG 
GAGATGATGG ATCTGGCCAA GCATCTTCCT GACGCTTTGA AAAAATTAGA AGATCCACAG 
AGGGTTTCTC TACTATTAAG TGCTATTTCA GAAAATCTGT ATTCATTGAG GAAGATGATA 
ATTGGTTCTC CTGAGGCCGA ATTGAGGCAC AGCACTAATG TTCACTTATT ATCATTGGGA 
GATCCAACTG CTGATGAAGT ACAGCAGAAA CAAATTAGAG AACATAAATT TTTTCCTAAA 
GTTAAAGATG AGACTTGGGA CACAACATTT GATAATTTAA TTAAACATGA TGAAGGAACC 
GAACCTCAAA ACAAAGTGAA ACAAAAAGAA GATGGACTTG AAGATGGATT AGAAGTTGGC 
AATAAATTGA AAACGAACAC AGAAAGAATT TGTTTGATGT CATTAGATAT AACAGAACAC 
GAACTCCAGA TTTTGGAACA GCAGGCTCAG GAAAAATTTC TTAATGATGT CACTTGCAAA 
TCTCCTGAGC ATTTATCTTC CAAAGATAAT GAAGAGGATC TATCTTATGT AATTGAAAGC 
GATGAAGATT TAGAAAAGGA AATGATTAAG TCTTTAGAAA ATTTAAATAC TGGCATGGCA 
GATCCAATTC ATTCAAAATG CATAAAAATG GAAGGAAATC TGGATGTTCC TTTTGAAGAT 
GATGAAGATG AGATTGAAGC TATTGAAGAA GAAGATGATA ATGTTGAAGA CTATTCATTA 
CCAAGACCTA ATGCAAGGCA AATTAATTTC CTCAAGATCT ATTTTGGCCA TTCCAGTTTT 
AAACCGGTTC AGTGGAAGGT GATTCATTCT GTATTGGAAG AAAGGAGAGA TAATGTTGTT 
GTGATGGCAA CTGGATATGG AAAGAGTTTG TGCTTCCAGT ACCCACCTGT GTATTCAGGG 
AGGATTGGTC TTGTCATCTC TCCTCTTATT TCTTTGATGG AAGACCAAGT GCTCCAGCTT 
AAGATGTCCA ATATTCCAGC TTGTTTTCTG GGATCAGCAC AGTCAAAAAA TGTTCTAGAA 
GATATTAAAT TAGGCAAATA TCGGATTGTA TACATAACTC CAGAATTCTG TTCTGGTAAC 
TTGTTCCTAC TCCAGCAACT TCAGGCTGAT ATTGGTATCA CACTTATTGC TGTGGATGAG 
GCTCACTGTA TTTCTGAATG GGGACATGAT TTTAGAAGTT CATTCAGGAC TTTGGGCTCC 
CTAAAGGCAA TACTCCCATC GGTTCCAATT GTTGCACTTA CTGCTACTGC AAGTTCTTCA 
ATCCGGGAAG ACATTGTACG CTGCTTAAAC CTGAAGAATC CTCAGATTAC CTGTACTGGT 
TTTGATCGAC CAAACTTGTA TTTAGAAGTT AGGCGAAAAA CAGGAAGCAT CCTTCAGGAT 
CTTGACCAAT TTCTTGTCCA AAAGACAAGT TCCATCTGGG AATTTGAAGG TCCAACTATC 
ATCTATTGTC CTTCCCGAAA AACAACAGAA CAAGTTACAG CTGAACTTAG GAAACTGAAG 
TTAGCCTGTG GAGCATACCA TGCAGGCTTG GATATTAAAT CAAGGAGAGA AATTCATCAT 
AGGTTCATGA GAGATGAAAT TCAGTGTGTT GTAGCTACCA TAGCTTTTGG AATGGGCATT 
AATAAAGCTG ACATTCGCAA AGTCATTCAT TATGGTGCTC CTAAGGAAAT GGAATCATAT 
TACCAGGAGA TTGGGAGAGC TGGCCGTGAT GGACTTCAGA GTTCTTGTCA TGTACTCTGG 
GCTCCAGCAG ACATTTGCTT AAATAGGCAC CGCCTTAGTG AGATAACCAG TGAAAAGTTT 
CGATTATACA AATTAAAGAT GATGGCGAAG ATGGAAAAAT ATCTTCATTC CACAAGATGT 
AGGCGACAAC TCATCTTGTC TCATTTTGAA GACAAACAAC TACGAAAGGC CTCCGTGGGT 
ATTATGGGAA CTGAACAATG CTGTGATAAT TGCAGATCCA GATTGGATCA ATGCTCTTCC 
ATTGATGATT CAGATGACGC ATCACAGGAT TTTGGTCCAC AAACATTCCA GCTATTATCT 
GCTGTGAAAA TCTTAGGAGA AAAGTTTGGA ATTGGGCTTC CGATTTTATT TCTCCAAGGA 
TCTAATTCCC AGCGTCTTGA AGATAAGTAT CGCAGACACA GTTTATTTGG CACTGGCAAG 
GATCAAAAAG AGAGTTGGTG GAAGGCTTTG TCCCGTCAGC TCATGGCTGA AGGATTCTTG 
GTAGAAGTTC CTGGGAGACA AAAATTTATA AGGATTTGCA CTCTTACAGA AAAGGGCAGA 
TGTTGGCTTC ATGAAGCCAA TAGAGAATTT CCTAAGAAAC TTAAACTTGA AGCTAATGAA 
GAATTGTGTC CGAGGGTGTT TCTTCTACCA AGTTCTAAAA CTGTACCACC TGGCGCTGAA 
CAACATTCCT CTAATCGAGT ACCAATTGAA TTAACTGCAG AGAAGAAGTC TAACTTGGAG 
AAGATGTATT CTTACAAAGC ATCTGATAAA ATTTCTTCTG GGAGTAACAT TCCTGAAAAA 
AGCATCATAG TACACTCACC AGGAAAATCT TACAAGTCTT CAGAACCTGT TATTTCAGCT 
GAAGAGCAGG AGACTCAGAC TGTGTTGTAT GGCATATTGG TGGAAGCCAG GCAGAAACAT 
GCCGGTAAAA TGGATGTTCC TCCAGCCATT CTAGCAACAA ATAAGATACT ATTGGAAATG 
GCCAAAATGA GACCTACTAC ATTTGAAAAT ATGAAAAGGA TCAATGGTGT GTCTGAAGGC 
AAAGCTACTA TGCTGACCCC TCTGTTGGAA GTCATCAAAC ATTTCTGTCA GATAAATAAT 
GTTCAGTCAG ACCTCTTTTC AAGTACAAAA CCTTGGGAAG AACCAAGGAA CAGTGTGGTG 
GAGGAAAATG AAACATGTTC CCTTTCACAG TCTGCGGCCA TCACGTATTC CTTATTCCAA 
GAAAAGAAGA TGTCTTTGAT AAGTTTAACT GAGGACAGGA TTATGCCTCT CACAGCAGTT 
GGAATGCACT TAACCCAAGC AGTGAAAGCT GGCTGTCCAC TTGATATGGA GCGAGCAGGC 
CTGACTCCAG AGGTTCAGAA GATTATTACT GATGTTATCC GAAACCCTCC CATCAACTCA 
GATATAAATA AAATTAAACT AATTAGAAAG CTGGTTCCTG AAAACATTGA CACGTACCTT 
ATCCACATGG TAATTGAGAT CCTGGAAAAA GACTCTGACA ACAGACTGTT GTCAGACGGG 
CTTTCATGTG CTTCCAACAG AAAGAGATAT TTCCCCAATT CTGAAGAGAA CTGTTCAAGT 
TCTAAAAGAA GTAGGGAAGA AGTAGGCACT AATACCAAGG TTGATGGAAA ACATCCTTAC 
GATTTTTCAT TGAAAGATGA AAGTCACAAA AATTCCCTAA GACTAACGTC TTGGAATCAA 
CCAACCAGCG ATTCAGAAAC AGATTTATTT ACAGATTCTC ACCCACAGAC TTCATCTATA 
ACCTCCAAGA GAAAATTACC TAAGTGGTTT GCCAAAGACA CTGAGACTTT AGCTGACACC 
AGCAGGAAAT CAGCAGCCAA AGCACAAAAG AAAGGTCTTT TTAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013975547.1
CDS	65..4432
Translation	MNEKKLERTPQPRKLPEWMSMQNETCTAVETKASIQKSVFEDDLSFLEFTGSIVYSYEASDCSFLSEDISMNLSTGGVVGFDMEWPPVYTKRKPSRVALIQLCVSENKCYLFHISSMSVFPQGLKMLLENEAIKKAGVGIKGDQRKLLCDFDIDLKNFVELTDVANEKLKCTETWSLNGLVKHLFGKQLLKDRSIRCSNWGDFPLTEDQKLYAATDAYAGLIIYQKLEILGNAVERFAINKKEEEILPIDVKKQLTSISEEMMDLAKHLPDALKKLEDPQRVSLLLSAISENLYSLRKMIIGSPEAELRHSTNVHLLSLGDPTADEVQQKQIREHKFFPKVKDETWDTTFDNLIKHDEGTEPQNKVKQKEDGLEDGLEVGNKLKTNTERICLMSLDITEHELQILEQQAQEKFLNDVTCKSPEHLSSKDNEEDLSYVIESDEDLEKEMIKSLENLNTGMADPIHSKCIKMEGNLDVPFEDDEDEIEAIEEEDDNVEDYSLPRPNARQINFLKIYFGHSSFKPVQWKVIHSVLEERRDNVVVMATGYGKSLCFQYPPVYSGRIGLVISPLISLMEDQVLQLKMSNIPACFLGSAQSKNVLEDIKLGKYRIVYITPEFCSGNLFLLQQLQADIGITLIAVDEAHCISEWGHDFRSSFRTLGSLKAILPSVPIVALTATASSSIREDIVRCLNLKNPQITCTGFDRPNLYLEVRRKTGSILQDLDQFLVQKTSSIWEFEGPTIIYCPSRKTTEQVTAELRKLKLACGAYHAGLDIKSRREIHHRFMRDEIQCVVATIAFGMGINKADIRKVIHYGAPKEMESYYQEIGRAGRDGLQSSCHVLWAPADICLNRHRLSEITSEKFRLYKLKMMAKMEKYLHSTRCRRQLILSHFEDKQLRKASVGIMGTEQCCDNCRSRLDQCSSIDDSDDASQDFGPQTFQLLSAVKILGEKFGIGLPILFLQGSNSQRLEDKYRRHSLFGTGKDQKESWWKALSRQLMAEGFLVEVPGRQKFIRICTLTEKGRCWLHEANREFPKKLKLEANEELCPRVFLLPSSKTVPPGAEQHSSNRVPIELTAEKKSNLEKMYSYKASDKISSGSNIPEKSIIVHSPGKSYKSSEPVISAEEQETQTVLYGILVEARQKHAGKMDVPPAILATNKILLEMAKMRPTTFENMKRINGVSEGKATMLTPLLEVIKHFCQINNVQSDLFSSTKPWEEPRNSVVEENETCSLSQSAAITYSLFQEKKMSLISLTEDRIMPLTAVGMHLTQAVKAGCPLDMERAGLTPEVQKIITDVIRNPPINSDINKIKLIRKLVPENIDTYLIHMVIEILEKDSDNRLLSDGLSCASNRKRYFPNSEENCSSSKRSREEVGTNTKVDGKHPYDFSLKDESHKNSLRLTSWNQPTSDSETDLFTDSHPQTSSITSKRKLPKWFAKDTETLADTSRKSAAKAQKKGLFS

Target ORF information:

RefSeq Version	XM_014120072.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris Werner syndrome RecQ like helicase (WRN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120072.2

ORF Insert Sequence:

ATGAATGAAA AAAAATTGGA AAGAACTCCA CAGCCGCGAA AACTTCCTGA ATGGATGTCT 
ATGCAGAATG AGACATGTAC TGCAGTAGAA ACAAAGGCAT CGATTCAGAA GAGTGTTTTT 
GAAGACGACC TCTCTTTCTT AGAATTCACT GGATCTATTG TTTATAGTTA TGAAGCTAGT 
GATTGTTCTT TTCTATCAGA AGATATTAGC ATGAATCTGT CTACTGGAGG TGTGGTGGGT 
TTTGACATGG AATGGCCACC AGTATACACT AAAAGGAAAC CGAGCCGAGT TGCACTGATT 
CAGTTATGTG TGTCAGAGAA CAAATGTTAC TTGTTTCATA TTTCTTCTAT GTCAGTTTTT 
CCTCAGGGGT TAAAAATGTT GCTTGAAAAT GAAGCAATTA AAAAAGCAGG TGTAGGAATC 
AAAGGAGATC AGCGGAAACT TCTATGTGAC TTTGACATCG ATTTGAAGAA TTTTGTGGAG 
CTGACAGATG TTGCCAATGA AAAGCTGAAA TGTACAGAGA CTTGGAGCCT CAATGGTTTG 
GTTAAACACC TCTTTGGCAA ACAGCTTCTG AAAGACAGAT CTATCCGTTG TAGCAATTGG 
GGTGATTTTC CTCTCACTGA GGATCAGAAA CTGTATGCAG CCACTGATGC GTATGCTGGT 
CTCATCATTT ATCAGAAATT AGAGATTTTA GGTAATGCTG TGGAAAGGTT TGCTATAAAT 
AAAAAAGAGG AGGAAATCCT ACCTATTGAT GTGAAGAAAC AATTGACTTC AATCTCTGAG 
GAGATGATGG ATCTGGCCAA GCATCTTCCT GACGCTTTGA AAAAATTAGA AGATCCACAG 
AGGGTTTCTC TACTATTAAG TGCTATTTCA GAAAATCTGT ATTCATTGAG GAAGATGATA 
ATTGGTTCTC CTGAGGCCGA ATTGAGGCAC AGCACTAATG TTCACTTATT ATCATTGGGA 
GATCCAACTG CTGATGAAGT ACAGCAGAAA CAAATTAGAG AACATAAATT TTTTCCTAAA 
GTTAAAGATG AGACTTGGGA CACAACATTT GATAATTTAA TTAAACATGA TGAAGGAACC 
GAACCTCAAA ACAAAGTGAA ACAAAAAGAA GATGGACTTG AAGATGGATT AGAAGTTGGC 
AATAAATTGA AAACGAACAC AGAAAGAATT TGTTTGATGT CATTAGATAT AACAGAACAC 
GAACTCCAGA TTTTGGAACA GCAGGCTCAG GAAAAATTTC TTAATGATGT CACTTGCAAA 
TCTCCTGAGC ATTTATCTTC CAAAGATAAT GAAGAGGATC TATCTTATGT AATTGAAAGC 
GATGAAGATT TAGAAAAGGA AATGATTAAG TCTTTAGAAA ATTTAAATAC TGGCATGGCA 
GATCCAATTC ATTCAAAATG CATAAAAATG GAAGGAAATC TGGATGTTCC TTTTGAAGAT 
GATGAAGATG AGATTGAAGC TATTGAAGAA GAAGATGATA ATGTTGAAGA CTATTCATTA 
CCAAGACCTA ATGCAAGGCA AATTAATTTC CTCAAGATCT ATTTTGGCCA TTCCAGTTTT 
AAACCGGTTC AGTGGAAGGT GATTCATTCT GTATTGGAAG AAAGGAGAGA TAATGTTGTT 
GTGATGGCAA CTGGATATGG AAAGAGTTTG TGCTTCCAGT ACCCACCTGT GTATTCAGGG 
AGGATTGGTC TTGTCATCTC TCCTCTTATT TCTTTGATGG AAGACCAAGT GCTCCAGCTT 
AAGATGTCCA ATATTCCAGC TTGTTTTCTG GGATCAGCAC AGTCAAAAAA TGTTCTAGAA 
GATATTAAAT TAGGCAAATA TCGGATTGTA TACATAACTC CAGAATTCTG TTCTGGTAAC 
TTGTTCCTAC TCCAGCAACT TCAGGCTGAT ATTGGTATCA CACTTATTGC TGTGGATGAG 
GCTCACTGTA TTTCTGAATG GGGACATGAT TTTAGAAGTT CATTCAGGAC TTTGGGCTCC 
CTAAAGGCAA TACTCCCATC GGTTCCAATT GTTGCACTTA CTGCTACTGC AAGTTCTTCA 
ATCCGGGAAG ACATTGTACG CTGCTTAAAC CTGAAGAATC CTCAGATTAC CTGTACTGGT 
TTTGATCGAC CAAACTTGTA TTTAGAAGTT AGGCGAAAAA CAGGAAGCAT CCTTCAGGAT 
CTTGACCAAT TTCTTGTCCA AAAGACAAGT TCCATCTGGG AATTTGAAGG TCCAACTATC 
ATCTATTGTC CTTCCCGAAA AACAACAGAA CAAGTTACAG CTGAACTTAG GAAACTGAAG 
TTAGCCTGTG GAGCATACCA TGCAGGCTTG GATATTAAAT CAAGGAGAGA AATTCATCAT 
AGGTTCATGA GAGATGAAAT TCAGTGTGTT GTAGCTACCA TAGCTTTTGG AATGGGCATT 
AATAAAGCTG ACATTCGCAA AGTCATTCAT TATGGTGCTC CTAAGGAAAT GGAATCATAT 
TACCAGGAGA TTGGGAGAGC TGGCCGTGAT GGACTTCAGA GTTCTTGTCA TGTACTCTGG 
GCTCCAGCAG ACATTTGCTT AAATAGGCAC CGCCTTAGTG AGATAACCAG TGAAAAGTTT 
CGATTATACA AATTAAAGAT GATGGCGAAG ATGGAAAAAT ATCTTCATTC CACAAGATGT 
AGGCGACAAC TCATCTTGTC TCATTTTGAA GACAAACAAC TACGAAAGGC CTCCGTGGGT 
ATTATGGGAA CTGAACAATG CTGTGATAAT TGCAGATCCA GATTGGATCA ATGCTCTTCC 
ATTGATGATT CAGATGACGC ATCACAGGAT TTTGGTCCAC AAACATTCCA GCTATTATCT 
GCTGTGAAAA TCTTAGGAGA AAAGTTTGGA ATTGGGCTTC CGATTTTATT TCTCCAAGGA 
TCTAATTCCC AGCGTCTTGA AGATAAGTAT CGCAGACACA GTTTATTTGG CACTGGCAAG 
GATCAAAAAG AGAGTTGGTG GAAGGCTTTG TCCCGTCAGC TCATGGCTGA AGGATTCTTG 
GTAGAAGTTC CTGGGAGACA AAAATTTATA AGGATTTGCA CTCTTACAGA AAAGGGCAGA 
TGTTGGCTTC ATGAAGCCAA TAGAGAATTT CCTAAGAAAC TTAAACTTGA AGCTAATGAA 
GAATTGTGTC CGAGGGTGTT TCTTCTACCA AGTTCTAAAA CTGTACCACC TGGCGCTGAA 
CAACATTCCT CTAATCGAGT ACCAATTGAA TTAACTGCAG AGAAGAAGTC TAACTTGGAG 
AAGATGTATT CTTACAAAGC ATCTGATAAA ATTTCTTCTG GGAGTAACAT TCCTGAAAAA 
AGCATCATAG TACACTCACC AGGAAAATCT TACAAGTCTT CAGAACCTGT TATTTCAGCT 
GAAGAGCAGG AGACTCAGAC TGTGTTGTAT GGCATATTGG TGGAAGCCAG GCAGAAACAT 
GCCGGTAAAA TGGATGTTCC TCCAGCCATT CTAGCAACAA ATAAGATACT ATTGGAAATG 
GCCAAAATGA GACCTACTAC ATTTGAAAAT ATGAAAAGGA TCAATGGTGT GTCTGAAGGC 
AAAGCTACTA TGCTGACCCC TCTGTTGGAA GTCATCAAAC ATTTCTGTCA GATAAATAAT 
GTTCAGTCAG ACCTCTTTTC AAGTACAAAA CCTTGGGAAG AACCAAGGAA CAGTGTGGTG 
GAGGAAAATG AAACATGTTC CCTTTCACAG TCTGCGGCCA TCACGTATTC CTTATTCCAA 
GAAAAGAAGA TGTCTTTGAT AAGTTTAACT GAGGACAGGA TTATGCCTCT CACAGCAGTT 
GGAATGCACT TAACCCAAGC AGTGAAAGCT GGCTGTCCAC TTGATATGGA GCGAGCAGGC 
CTGACTCCAG AGGTTCAGAA GATTATTACT GATGTTATCC GAAACCCTCC CATCAACTCA 
GATATAAATA AAATTAAACT AATTAGAAAG CTGGTTCCTG AAAACATTGA CACGTACCTT 
ATCCACATGG TAATTGAGAT CCTGGAAAAA GACTCTGACA ACAGACTGTT GTCAGACGGG 
CTTTCATGTG CTTCCAACAG AAAGAGATAT TTCCCCAATT CTGAAGAGAA CTGTTCAAGT 
TCTAAAAGAA GTAGGGAAGA AGTAGGCACT AATACCAAGG TTGATGGAAA ACATCCTTAC 
GATTTTTCAT TGAAAGATGA AAGTCACAAA AATTCCCTAA GACTAACGTC TTGGAATCAA 
CCAACCAGCG ATTCAGAAAC AGATTTATTT ACAGATTCTC ACCCACAGAC TTCATCTATA 
ACCTCCAAGA GAAAATTACC TAAGTGGTTT GCCAAAGACA CTGAGACTTT AGCTGACACC 
AGCAGGAAAT CAGCAGCCAA AGCACAAAAG AAAGGTCTTT TTAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WRN ( XM_014120073.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013975548.1 Canis lupus familiaris Werner syndrome, RecQ helicase-like (WRN), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe40574
Clone ID Related Accession (Same CDS sequence)	XM_014120073.1
Accession Version	XM_014120073.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	Werner syndrome ATP-dependent helicase isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726087.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCTGT CTACTGGAGG TGTGGTGGGT TTTGACATGG AATGGCCACC AGTATACACT 
AAAAGGAAAC CGAGCCGAGT TGCACTGATT CAGTTATGTG TGTCAGAGAA CAAATGTTAC 
TTGTTTCATA TTTCTTCTAT GTCAGTTTTT CCTCAGGGGT TAAAAATGTT GCTTGAAAAT 
GAAGCAATTA AAAAAGCAGG TGTAGGAATC AAAGGAGATC AGCGGAAACT TCTATGTGAC 
TTTGACATCG ATTTGAAGAA TTTTGTGGAG CTGACAGATG TTGCCAATGA AAAGCTGAAA 
TGTACAGAGA CTTGGAGCCT CAATGGTTTG GTTAAACACC TCTTTGGCAA ACAGCTTCTG 
AAAGACAGAT CTATCCGTTG TAGCAATTGG GGTGATTTTC CTCTCACTGA GGATCAGAAA 
CTGTATGCAG CCACTGATGC GTATGCTGGT CTCATCATTT ATCAGAAATT AGAGATTTTA 
GGTAATGCTG TGGAAAGGTT TGCTATAAAT AAAAAAGAGG AGGAAATCCT ACCTATTGAT 
GTGAAGAAAC AATTGACTTC AATCTCTGAG GAGATGATGG ATCTGGCCAA GCATCTTCCT 
GACGCTTTGA AAAAATTAGA AGATCCACAG AGGGTTTCTC TACTATTAAG TGCTATTTCA 
GAAAATCTGT ATTCATTGAG GAAGATGATA ATTGGTTCTC CTGAGGCCGA ATTGAGGCAC 
AGCACTAATG TTCACTTATT ATCATTGGGA GATCCAACTG CTGATGAAGT ACAGCAGAAA 
CAAATTAGAG AACATAAATT TTTTCCTAAA GTTAAAGATG AGACTTGGGA CACAACATTT 
GATAATTTAA TTAAACATGA TGAAGGAACC GAACCTCAAA ACAAAGTGAA ACAAAAAGAA 
GATGGACTTG AAGATGGATT AGAAGTTGGC AATAAATTGA AAACGAACAC AGAAAGAATT 
TGTTTGATGT CATTAGATAT AACAGAACAC GAACTCCAGA TTTTGGAACA GCAGGCTCAG 
GAAAAATTTC TTAATGATGT CACTTGCAAA TCTCCTGAGC ATTTATCTTC CAAAGATAAT 
GAAGAGGATC TATCTTATGT AATTGAAAGC GATGAAGATT TAGAAAAGGA AATGATTAAG 
TCTTTAGAAA ATTTAAATAC TGGCATGGCA GATCCAATTC ATTCAAAATG CATAAAAATG 
GAAGGAAATC TGGATGTTCC TTTTGAAGAT GATGAAGATG AGATTGAAGC TATTGAAGAA 
GAAGATGATA ATGTTGAAGA CTATTCATTA CCAAGACCTA ATGCAAGGCA AATTAATTTC 
CTCAAGATCT ATTTTGGCCA TTCCAGTTTT AAACCGGTTC AGTGGAAGGT GATTCATTCT 
GTATTGGAAG AAAGGAGAGA TAATGTTGTT GTGATGGCAA CTGGATATGG AAAGAGTTTG 
TGCTTCCAGT ACCCACCTGT GTATTCAGGG AGGATTGGTC TTGTCATCTC TCCTCTTATT 
TCTTTGATGG AAGACCAAGT GCTCCAGCTT AAGATGTCCA ATATTCCAGC TTGTTTTCTG 
GGATCAGCAC AGTCAAAAAA TGTTCTAGAA GATATTAAAT TAGGCAAATA TCGGATTGTA 
TACATAACTC CAGAATTCTG TTCTGGTAAC TTGTTCCTAC TCCAGCAACT TCAGGCTGAT 
ATTGGTATCA CACTTATTGC TGTGGATGAG GCTCACTGTA TTTCTGAATG GGGACATGAT 
TTTAGAAGTT CATTCAGGAC TTTGGGCTCC CTAAAGGCAA TACTCCCATC GGTTCCAATT 
GTTGCACTTA CTGCTACTGC AAGTTCTTCA ATCCGGGAAG ACATTGTACG CTGCTTAAAC 
CTGAAGAATC CTCAGATTAC CTGTACTGGT TTTGATCGAC CAAACTTGTA TTTAGAAGTT 
AGGCGAAAAA CAGGAAGCAT CCTTCAGGAT CTTGACCAAT TTCTTGTCCA AAAGACAAGT 
TCCATCTGGG AATTTGAAGG TCCAACTATC ATCTATTGTC CTTCCCGAAA AACAACAGAA 
CAAGTTACAG CTGAACTTAG GAAACTGAAG TTAGCCTGTG GAGCATACCA TGCAGGCTTG 
GATATTAAAT CAAGGAGAGA AATTCATCAT AGGTTCATGA GAGATGAAAT TCAGTGTGTT 
GTAGCTACCA TAGCTTTTGG AATGGGCATT AATAAAGCTG ACATTCGCAA AGTCATTCAT 
TATGGTGCTC CTAAGGAAAT GGAATCATAT TACCAGGAGA TTGGGAGAGC TGGCCGTGAT 
GGACTTCAGA GTTCTTGTCA TGTACTCTGG GCTCCAGCAG ACATTTGCTT AAATAGGCAC 
CGCCTTAGTG AGATAACCAG TGAAAAGTTT CGATTATACA AATTAAAGAT GATGGCGAAG 
ATGGAAAAAT ATCTTCATTC CACAAGATGT AGGCGACAAC TCATCTTGTC TCATTTTGAA 
GACAAACAAC TACGAAAGGC CTCCGTGGGT ATTATGGGAA CTGAACAATG CTGTGATAAT 
TGCAGATCCA GATTGGATCA ATGCTCTTCC ATTGATGATT CAGATGACGC ATCACAGGAT 
TTTGGTCCAC AAACATTCCA GCTATTATCT GCTGTGAAAA TCTTAGGAGA AAAGTTTGGA 
ATTGGGCTTC CGATTTTATT TCTCCAAGGA TCTAATTCCC AGCGTCTTGA AGATAAGTAT 
CGCAGACACA GTTTATTTGG CACTGGCAAG GATCAAAAAG AGAGTTGGTG GAAGGCTTTG 
TCCCGTCAGC TCATGGCTGA AGGATTCTTG GTAGAAGTTC CTGGGAGACA AAAATTTATA 
AGGATTTGCA CTCTTACAGA AAAGGGCAGA TGTTGGCTTC ATGAAGCCAA TAGAGAATTT 
CCTAAGAAAC TTAAACTTGA AGCTAATGAA GAATTGTGTC CGAGGGTGTT TCTTCTACCA 
AGTTCTAAAA CTGTACCACC TGGCGCTGAA CAACATTCCT CTAATCGAGT ACCAATTGAA 
TTAACTGCAG AGAAGAAGTC TAACTTGGAG AAGATGTATT CTTACAAAGC ATCTGATAAA 
ATTTCTTCTG GGAGTAACAT TCCTGAAAAA AGCATCATAG TACACTCACC AGGAAAATCT 
TACAAGTCTT CAGAACCTGT TATTTCAGCT GAAGAGCAGG AGACTCAGAC TGTGTTGTAT 
GGCATATTGG TGGAAGCCAG GCAGAAACAT GCCGGTAAAA TGGATGTTCC TCCAGCCATT 
CTAGCAACAA ATAAGATACT ATTGGAAATG GCCAAAATGA GACCTACTAC ATTTGAAAAT 
ATGAAAAGGA TCAATGGTGT GTCTGAAGGC AAAGCTACTA TGCTGACCCC TCTGTTGGAA 
GTCATCAAAC ATTTCTGTCA GATAAATAAT GTTCAGTCAG ACCTCTTTTC AAGTACAAAA 
CCTTGGGAAG AACCAAGGAA CAGTGTGGTG GAGGAAAATG AAACATGTTC CCTTTCACAG 
TCTGCGGCCA TCACGTATTC CTTATTCCAA GAAAAGAAGA TGTCTTTGAT AAGTTTAACT 
GAGGACAGGA TTATGCCTCT CACAGCAGTT GGAATGCACT TAACCCAAGC AGTGAAAGCT 
GGCTGTCCAC TTGATATGGA GCGAGCAGGC CTGACTCCAG AGGTTCAGAA GATTATTACT 
GATGTTATCC GAAACCCTCC CATCAACTCA GATATAAATA AAATTAAACT AATTAGAAAG 
CTGGTTCCTG AAAACATTGA CACGTACCTT ATCCACATGG TAATTGAGAT CCTGGAAAAA 
GACTCTGACA ACAGACTGTT GTCAGACGGG CTTTCATGTG CTTCCAACAG AAAGAGATAT 
TTCCCCAATT CTGAAGAGAA CTGTTCAAGT TCTAAAAGAA GTAGGGAAGA AGTAGGCACT 
AATACCAAGG TTGATGGAAA ACATCCTTAC GATTTTTCAT TGAAAGATGA AAGTCACAAA 
AATTCCCTAA GACTAACGTC TTGGAATCAA CCAACCAGCG ATTCAGAAAC AGATTTATTT 
ACAGATTCTC ACCCACAGAC TTCATCTATA ACCTCCAAGA GAAAATTACC TAAGTGGTTT 
GCCAAAGACA CTGAGACTTT AGCTGACACC AGCAGGAAAT CAGCAGCCAA AGCACAAAAG 
AAAGGTCTTT TTAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013975548.1
CDS	89..4246
Translation	MNLSTGGVVGFDMEWPPVYTKRKPSRVALIQLCVSENKCYLFHISSMSVFPQGLKMLLENEAIKKAGVGIKGDQRKLLCDFDIDLKNFVELTDVANEKLKCTETWSLNGLVKHLFGKQLLKDRSIRCSNWGDFPLTEDQKLYAATDAYAGLIIYQKLEILGNAVERFAINKKEEEILPIDVKKQLTSISEEMMDLAKHLPDALKKLEDPQRVSLLLSAISENLYSLRKMIIGSPEAELRHSTNVHLLSLGDPTADEVQQKQIREHKFFPKVKDETWDTTFDNLIKHDEGTEPQNKVKQKEDGLEDGLEVGNKLKTNTERICLMSLDITEHELQILEQQAQEKFLNDVTCKSPEHLSSKDNEEDLSYVIESDEDLEKEMIKSLENLNTGMADPIHSKCIKMEGNLDVPFEDDEDEIEAIEEEDDNVEDYSLPRPNARQINFLKIYFGHSSFKPVQWKVIHSVLEERRDNVVVMATGYGKSLCFQYPPVYSGRIGLVISPLISLMEDQVLQLKMSNIPACFLGSAQSKNVLEDIKLGKYRIVYITPEFCSGNLFLLQQLQADIGITLIAVDEAHCISEWGHDFRSSFRTLGSLKAILPSVPIVALTATASSSIREDIVRCLNLKNPQITCTGFDRPNLYLEVRRKTGSILQDLDQFLVQKTSSIWEFEGPTIIYCPSRKTTEQVTAELRKLKLACGAYHAGLDIKSRREIHHRFMRDEIQCVVATIAFGMGINKADIRKVIHYGAPKEMESYYQEIGRAGRDGLQSSCHVLWAPADICLNRHRLSEITSEKFRLYKLKMMAKMEKYLHSTRCRRQLILSHFEDKQLRKASVGIMGTEQCCDNCRSRLDQCSSIDDSDDASQDFGPQTFQLLSAVKILGEKFGIGLPILFLQGSNSQRLEDKYRRHSLFGTGKDQKESWWKALSRQLMAEGFLVEVPGRQKFIRICTLTEKGRCWLHEANREFPKKLKLEANEELCPRVFLLPSSKTVPPGAEQHSSNRVPIELTAEKKSNLEKMYSYKASDKISSGSNIPEKSIIVHSPGKSYKSSEPVISAEEQETQTVLYGILVEARQKHAGKMDVPPAILATNKILLEMAKMRPTTFENMKRINGVSEGKATMLTPLLEVIKHFCQINNVQSDLFSSTKPWEEPRNSVVEENETCSLSQSAAITYSLFQEKKMSLISLTEDRIMPLTAVGMHLTQAVKAGCPLDMERAGLTPEVQKIITDVIRNPPINSDINKIKLIRKLVPENIDTYLIHMVIEILEKDSDNRLLSDGLSCASNRKRYFPNSEENCSSSKRSREEVGTNTKVDGKHPYDFSLKDESHKNSLRLTSWNQPTSDSETDLFTDSHPQTSSITSKRKLPKWFAKDTETLADTSRKSAAKAQKKGLFS

Target ORF information:

RefSeq Version	XM_014120073.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris Werner syndrome, RecQ helicase-like (WRN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120073.1

ORF Insert Sequence:

ATGAATCTGT CTACTGGAGG TGTGGTGGGT TTTGACATGG AATGGCCACC AGTATACACT 
AAAAGGAAAC CGAGCCGAGT TGCACTGATT CAGTTATGTG TGTCAGAGAA CAAATGTTAC 
TTGTTTCATA TTTCTTCTAT GTCAGTTTTT CCTCAGGGGT TAAAAATGTT GCTTGAAAAT 
GAAGCAATTA AAAAAGCAGG TGTAGGAATC AAAGGAGATC AGCGGAAACT TCTATGTGAC 
TTTGACATCG ATTTGAAGAA TTTTGTGGAG CTGACAGATG TTGCCAATGA AAAGCTGAAA 
TGTACAGAGA CTTGGAGCCT CAATGGTTTG GTTAAACACC TCTTTGGCAA ACAGCTTCTG 
AAAGACAGAT CTATCCGTTG TAGCAATTGG GGTGATTTTC CTCTCACTGA GGATCAGAAA 
CTGTATGCAG CCACTGATGC GTATGCTGGT CTCATCATTT ATCAGAAATT AGAGATTTTA 
GGTAATGCTG TGGAAAGGTT TGCTATAAAT AAAAAAGAGG AGGAAATCCT ACCTATTGAT 
GTGAAGAAAC AATTGACTTC AATCTCTGAG GAGATGATGG ATCTGGCCAA GCATCTTCCT 
GACGCTTTGA AAAAATTAGA AGATCCACAG AGGGTTTCTC TACTATTAAG TGCTATTTCA 
GAAAATCTGT ATTCATTGAG GAAGATGATA ATTGGTTCTC CTGAGGCCGA ATTGAGGCAC 
AGCACTAATG TTCACTTATT ATCATTGGGA GATCCAACTG CTGATGAAGT ACAGCAGAAA 
CAAATTAGAG AACATAAATT TTTTCCTAAA GTTAAAGATG AGACTTGGGA CACAACATTT 
GATAATTTAA TTAAACATGA TGAAGGAACC GAACCTCAAA ACAAAGTGAA ACAAAAAGAA 
GATGGACTTG AAGATGGATT AGAAGTTGGC AATAAATTGA AAACGAACAC AGAAAGAATT 
TGTTTGATGT CATTAGATAT AACAGAACAC GAACTCCAGA TTTTGGAACA GCAGGCTCAG 
GAAAAATTTC TTAATGATGT CACTTGCAAA TCTCCTGAGC ATTTATCTTC CAAAGATAAT 
GAAGAGGATC TATCTTATGT AATTGAAAGC GATGAAGATT TAGAAAAGGA AATGATTAAG 
TCTTTAGAAA ATTTAAATAC TGGCATGGCA GATCCAATTC ATTCAAAATG CATAAAAATG 
GAAGGAAATC TGGATGTTCC TTTTGAAGAT GATGAAGATG AGATTGAAGC TATTGAAGAA 
GAAGATGATA ATGTTGAAGA CTATTCATTA CCAAGACCTA ATGCAAGGCA AATTAATTTC 
CTCAAGATCT ATTTTGGCCA TTCCAGTTTT AAACCGGTTC AGTGGAAGGT GATTCATTCT 
GTATTGGAAG AAAGGAGAGA TAATGTTGTT GTGATGGCAA CTGGATATGG AAAGAGTTTG 
TGCTTCCAGT ACCCACCTGT GTATTCAGGG AGGATTGGTC TTGTCATCTC TCCTCTTATT 
TCTTTGATGG AAGACCAAGT GCTCCAGCTT AAGATGTCCA ATATTCCAGC TTGTTTTCTG 
GGATCAGCAC AGTCAAAAAA TGTTCTAGAA GATATTAAAT TAGGCAAATA TCGGATTGTA 
TACATAACTC CAGAATTCTG TTCTGGTAAC TTGTTCCTAC TCCAGCAACT TCAGGCTGAT 
ATTGGTATCA CACTTATTGC TGTGGATGAG GCTCACTGTA TTTCTGAATG GGGACATGAT 
TTTAGAAGTT CATTCAGGAC TTTGGGCTCC CTAAAGGCAA TACTCCCATC GGTTCCAATT 
GTTGCACTTA CTGCTACTGC AAGTTCTTCA ATCCGGGAAG ACATTGTACG CTGCTTAAAC 
CTGAAGAATC CTCAGATTAC CTGTACTGGT TTTGATCGAC CAAACTTGTA TTTAGAAGTT 
AGGCGAAAAA CAGGAAGCAT CCTTCAGGAT CTTGACCAAT TTCTTGTCCA AAAGACAAGT 
TCCATCTGGG AATTTGAAGG TCCAACTATC ATCTATTGTC CTTCCCGAAA AACAACAGAA 
CAAGTTACAG CTGAACTTAG GAAACTGAAG TTAGCCTGTG GAGCATACCA TGCAGGCTTG 
GATATTAAAT CAAGGAGAGA AATTCATCAT AGGTTCATGA GAGATGAAAT TCAGTGTGTT 
GTAGCTACCA TAGCTTTTGG AATGGGCATT AATAAAGCTG ACATTCGCAA AGTCATTCAT 
TATGGTGCTC CTAAGGAAAT GGAATCATAT TACCAGGAGA TTGGGAGAGC TGGCCGTGAT 
GGACTTCAGA GTTCTTGTCA TGTACTCTGG GCTCCAGCAG ACATTTGCTT AAATAGGCAC 
CGCCTTAGTG AGATAACCAG TGAAAAGTTT CGATTATACA AATTAAAGAT GATGGCGAAG 
ATGGAAAAAT ATCTTCATTC CACAAGATGT AGGCGACAAC TCATCTTGTC TCATTTTGAA 
GACAAACAAC TACGAAAGGC CTCCGTGGGT ATTATGGGAA CTGAACAATG CTGTGATAAT 
TGCAGATCCA GATTGGATCA ATGCTCTTCC ATTGATGATT CAGATGACGC ATCACAGGAT 
TTTGGTCCAC AAACATTCCA GCTATTATCT GCTGTGAAAA TCTTAGGAGA AAAGTTTGGA 
ATTGGGCTTC CGATTTTATT TCTCCAAGGA TCTAATTCCC AGCGTCTTGA AGATAAGTAT 
CGCAGACACA GTTTATTTGG CACTGGCAAG GATCAAAAAG AGAGTTGGTG GAAGGCTTTG 
TCCCGTCAGC TCATGGCTGA AGGATTCTTG GTAGAAGTTC CTGGGAGACA AAAATTTATA 
AGGATTTGCA CTCTTACAGA AAAGGGCAGA TGTTGGCTTC ATGAAGCCAA TAGAGAATTT 
CCTAAGAAAC TTAAACTTGA AGCTAATGAA GAATTGTGTC CGAGGGTGTT TCTTCTACCA 
AGTTCTAAAA CTGTACCACC TGGCGCTGAA CAACATTCCT CTAATCGAGT ACCAATTGAA 
TTAACTGCAG AGAAGAAGTC TAACTTGGAG AAGATGTATT CTTACAAAGC ATCTGATAAA 
ATTTCTTCTG GGAGTAACAT TCCTGAAAAA AGCATCATAG TACACTCACC AGGAAAATCT 
TACAAGTCTT CAGAACCTGT TATTTCAGCT GAAGAGCAGG AGACTCAGAC TGTGTTGTAT 
GGCATATTGG TGGAAGCCAG GCAGAAACAT GCCGGTAAAA TGGATGTTCC TCCAGCCATT 
CTAGCAACAA ATAAGATACT ATTGGAAATG GCCAAAATGA GACCTACTAC ATTTGAAAAT 
ATGAAAAGGA TCAATGGTGT GTCTGAAGGC AAAGCTACTA TGCTGACCCC TCTGTTGGAA 
GTCATCAAAC ATTTCTGTCA GATAAATAAT GTTCAGTCAG ACCTCTTTTC AAGTACAAAA 
CCTTGGGAAG AACCAAGGAA CAGTGTGGTG GAGGAAAATG AAACATGTTC CCTTTCACAG 
TCTGCGGCCA TCACGTATTC CTTATTCCAA GAAAAGAAGA TGTCTTTGAT AAGTTTAACT 
GAGGACAGGA TTATGCCTCT CACAGCAGTT GGAATGCACT TAACCCAAGC AGTGAAAGCT 
GGCTGTCCAC TTGATATGGA GCGAGCAGGC CTGACTCCAG AGGTTCAGAA GATTATTACT 
GATGTTATCC GAAACCCTCC CATCAACTCA GATATAAATA AAATTAAACT AATTAGAAAG 
CTGGTTCCTG AAAACATTGA CACGTACCTT ATCCACATGG TAATTGAGAT CCTGGAAAAA 
GACTCTGACA ACAGACTGTT GTCAGACGGG CTTTCATGTG CTTCCAACAG AAAGAGATAT 
TTCCCCAATT CTGAAGAGAA CTGTTCAAGT TCTAAAAGAA GTAGGGAAGA AGTAGGCACT 
AATACCAAGG TTGATGGAAA ACATCCTTAC GATTTTTCAT TGAAAGATGA AAGTCACAAA 
AATTCCCTAA GACTAACGTC TTGGAATCAA CCAACCAGCG ATTCAGAAAC AGATTTATTT 
ACAGATTCTC ACCCACAGAC TTCATCTATA ACCTCCAAGA GAAAATTACC TAAGTGGTTT 
GCCAAAGACA CTGAGACTTT AGCTGACACC AGCAGGAAAT CAGCAGCCAA AGCACAAAAG 
AAAGGTCTTT TTAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WRN ( XM_014120074.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013975549.1 Canis lupus familiaris Werner syndrome, RecQ helicase-like (WRN), transcript variant X4, mRNA.

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CloneID	OCe40575
Clone ID Related Accession (Same CDS sequence)	XM_014120074.1 , XM_014120074.2
Accession Version	XM_014120074.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	Werner syndrome ATP-dependent helicase isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726087.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCTTG AAAATGAAGC AATTAAAAAA GCAGGTGTAG GAATCAAAGG AGATCAGCGG 
AAACTTCTAT GTGACTTTGA CATCGATTTG AAGAATTTTG TGGAGCTGAC AGATGTTGCC 
AATGAAAAGC TGAAATGTAC AGAGACTTGG AGCCTCAATG GTTTGGTTAA ACACCTCTTT 
GGCAAACAGC TTCTGAAAGA CAGATCTATC CGTTGTAGCA ATTGGGGTGA TTTTCCTCTC 
ACTGAGGATC AGAAACTGTA TGCAGCCACT GATGCGTATG CTGGTCTCAT CATTTATCAG 
AAATTAGAGA TTTTAGGTAA TGCTGTGGAA AGGTTTGCTA TAAATAAAAA AGAGGAGGAA 
ATCCTACCTA TTGATGTGAA GAAACAATTG ACTTCAATCT CTGAGGAGAT GATGGATCTG 
GCCAAGCATC TTCCTGACGC TTTGAAAAAA TTAGAAGATC CACAGAGGGT TTCTCTACTA 
TTAAGTGCTA TTTCAGAAAA TCTGTATTCA TTGAGGAAGA TGATAATTGG TTCTCCTGAG 
GCCGAATTGA GGCACAGCAC TAATGTTCAC TTATTATCAT TGGGAGATCC AACTGCTGAT 
GAAGTACAGC AGAAACAAAT TAGAGAACAT AAATTTTTTC CTAAAGTTAA AGATGAGACT 
TGGGACACAA CATTTGATAA TTTAATTAAA CATGATGAAG GAACCGAACC TCAAAACAAA 
GTGAAACAAA AAGAAGATGG ACTTGAAGAT GGATTAGAAG TTGGCAATAA ATTGAAAACG 
AACACAGAAA GAATTTGTTT GATGTCATTA GATATAACAG AACACGAACT CCAGATTTTG 
GAACAGCAGG CTCAGGAAAA ATTTCTTAAT GATGTCACTT GCAAATCTCC TGAGCATTTA 
TCTTCCAAAG ATAATGAAGA GGATCTATCT TATGTAATTG AAAGCGATGA AGATTTAGAA 
AAGGAAATGA TTAAGTCTTT AGAAAATTTA AATACTGGCA TGGCAGATCC AATTCATTCA 
AAATGCATAA AAATGGAAGG AAATCTGGAT GTTCCTTTTG AAGATGATGA AGATGAGATT 
GAAGCTATTG AAGAAGAAGA TGATAATGTT GAAGACTATT CATTACCAAG ACCTAATGCA 
AGGCAAATTA ATTTCCTCAA GATCTATTTT GGCCATTCCA GTTTTAAACC GGTTCAGTGG 
AAGGTGATTC ATTCTGTATT GGAAGAAAGG AGAGATAATG TTGTTGTGAT GGCAACTGGA 
TATGGAAAGA GTTTGTGCTT CCAGTACCCA CCTGTGTATT CAGGGAGGAT TGGTCTTGTC 
ATCTCTCCTC TTATTTCTTT GATGGAAGAC CAAGTGCTCC AGCTTAAGAT GTCCAATATT 
CCAGCTTGTT TTCTGGGATC AGCACAGTCA AAAAATGTTC TAGAAGATAT TAAATTAGGC 
AAATATCGGA TTGTATACAT AACTCCAGAA TTCTGTTCTG GTAACTTGTT CCTACTCCAG 
CAACTTCAGG CTGATATTGG TATCACACTT ATTGCTGTGG ATGAGGCTCA CTGTATTTCT 
GAATGGGGAC ATGATTTTAG AAGTTCATTC AGGACTTTGG GCTCCCTAAA GGCAATACTC 
CCATCGGTTC CAATTGTTGC ACTTACTGCT ACTGCAAGTT CTTCAATCCG GGAAGACATT 
GTACGCTGCT TAAACCTGAA GAATCCTCAG ATTACCTGTA CTGGTTTTGA TCGACCAAAC 
TTGTATTTAG AAGTTAGGCG AAAAACAGGA AGCATCCTTC AGGATCTTGA CCAATTTCTT 
GTCCAAAAGA CAAGTTCCAT CTGGGAATTT GAAGGTCCAA CTATCATCTA TTGTCCTTCC 
CGAAAAACAA CAGAACAAGT TACAGCTGAA CTTAGGAAAC TGAAGTTAGC CTGTGGAGCA 
TACCATGCAG GCTTGGATAT TAAATCAAGG AGAGAAATTC ATCATAGGTT CATGAGAGAT 
GAAATTCAGT GTGTTGTAGC TACCATAGCT TTTGGAATGG GCATTAATAA AGCTGACATT 
CGCAAAGTCA TTCATTATGG TGCTCCTAAG GAAATGGAAT CATATTACCA GGAGATTGGG 
AGAGCTGGCC GTGATGGACT TCAGAGTTCT TGTCATGTAC TCTGGGCTCC AGCAGACATT 
TGCTTAAATA GGCACCGCCT TAGTGAGATA ACCAGTGAAA AGTTTCGATT ATACAAATTA 
AAGATGATGG CGAAGATGGA AAAATATCTT CATTCCACAA GATGTAGGCG ACAACTCATC 
TTGTCTCATT TTGAAGACAA ACAACTACGA AAGGCCTCCG TGGGTATTAT GGGAACTGAA 
CAATGCTGTG ATAATTGCAG ATCCAGATTG GATCAATGCT CTTCCATTGA TGATTCAGAT 
GACGCATCAC AGGATTTTGG TCCACAAACA TTCCAGCTAT TATCTGCTGT GAAAATCTTA 
GGAGAAAAGT TTGGAATTGG GCTTCCGATT TTATTTCTCC AAGGATCTAA TTCCCAGCGT 
CTTGAAGATA AGTATCGCAG ACACAGTTTA TTTGGCACTG GCAAGGATCA AAAAGAGAGT 
TGGTGGAAGG CTTTGTCCCG TCAGCTCATG GCTGAAGGAT TCTTGGTAGA AGTTCCTGGG 
AGACAAAAAT TTATAAGGAT TTGCACTCTT ACAGAAAAGG GCAGATGTTG GCTTCATGAA 
GCCAATAGAG AATTTCCTAA GAAACTTAAA CTTGAAGCTA ATGAAGAATT GTGTCCGAGG 
GTGTTTCTTC TACCAAGTTC TAAAACTGTA CCACCTGGCG CTGAACAACA TTCCTCTAAT 
CGAGTACCAA TTGAATTAAC TGCAGAGAAG AAGTCTAACT TGGAGAAGAT GTATTCTTAC 
AAAGCATCTG ATAAAATTTC TTCTGGGAGT AACATTCCTG AAAAAAGCAT CATAGTACAC 
TCACCAGGAA AATCTTACAA GTCTTCAGAA CCTGTTATTT CAGCTGAAGA GCAGGAGACT 
CAGACTGTGT TGTATGGCAT ATTGGTGGAA GCCAGGCAGA AACATGCCGG TAAAATGGAT 
GTTCCTCCAG CCATTCTAGC AACAAATAAG ATACTATTGG AAATGGCCAA AATGAGACCT 
ACTACATTTG AAAATATGAA AAGGATCAAT GGTGTGTCTG AAGGCAAAGC TACTATGCTG 
ACCCCTCTGT TGGAAGTCAT CAAACATTTC TGTCAGATAA ATAATGTTCA GTCAGACCTC 
TTTTCAAGTA CAAAACCTTG GGAAGAACCA AGGAACAGTG TGGTGGAGGA AAATGAAACA 
TGTTCCCTTT CACAGTCTGC GGCCATCACG TATTCCTTAT TCCAAGAAAA GAAGATGTCT 
TTGATAAGTT TAACTGAGGA CAGGATTATG CCTCTCACAG CAGTTGGAAT GCACTTAACC 
CAAGCAGTGA AAGCTGGCTG TCCACTTGAT ATGGAGCGAG CAGGCCTGAC TCCAGAGGTT 
CAGAAGATTA TTACTGATGT TATCCGAAAC CCTCCCATCA ACTCAGATAT AAATAAAATT 
AAACTAATTA GAAAGCTGGT TCCTGAAAAC ATTGACACGT ACCTTATCCA CATGGTAATT 
GAGATCCTGG AAAAAGACTC TGACAACAGA CTGTTGTCAG ACGGGCTTTC ATGTGCTTCC 
AACAGAAAGA GATATTTCCC CAATTCTGAA GAGAACTGTT CAAGTTCTAA AAGAAGTAGG 
GAAGAAGTAG GCACTAATAC CAAGGTTGAT GGAAAACATC CTTACGATTT TTCATTGAAA 
GATGAAAGTC ACAAAAATTC CCTAAGACTA ACGTCTTGGA ATCAACCAAC CAGCGATTCA 
GAAACAGATT TATTTACAGA TTCTCACCCA CAGACTTCAT CTATAACCTC CAAGAGAAAA 
TTACCTAAGT GGTTTGCCAA AGACACTGAG ACTTTAGCTG ACACCAGCAG GAAATCAGCA 
GCCAAAGCAC AAAAGAAAGG TCTTTTTAGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013975549.1
CDS	67..4059
Translation	MLLENEAIKKAGVGIKGDQRKLLCDFDIDLKNFVELTDVANEKLKCTETWSLNGLVKHLFGKQLLKDRSIRCSNWGDFPLTEDQKLYAATDAYAGLIIYQKLEILGNAVERFAINKKEEEILPIDVKKQLTSISEEMMDLAKHLPDALKKLEDPQRVSLLLSAISENLYSLRKMIIGSPEAELRHSTNVHLLSLGDPTADEVQQKQIREHKFFPKVKDETWDTTFDNLIKHDEGTEPQNKVKQKEDGLEDGLEVGNKLKTNTERICLMSLDITEHELQILEQQAQEKFLNDVTCKSPEHLSSKDNEEDLSYVIESDEDLEKEMIKSLENLNTGMADPIHSKCIKMEGNLDVPFEDDEDEIEAIEEEDDNVEDYSLPRPNARQINFLKIYFGHSSFKPVQWKVIHSVLEERRDNVVVMATGYGKSLCFQYPPVYSGRIGLVISPLISLMEDQVLQLKMSNIPACFLGSAQSKNVLEDIKLGKYRIVYITPEFCSGNLFLLQQLQADIGITLIAVDEAHCISEWGHDFRSSFRTLGSLKAILPSVPIVALTATASSSIREDIVRCLNLKNPQITCTGFDRPNLYLEVRRKTGSILQDLDQFLVQKTSSIWEFEGPTIIYCPSRKTTEQVTAELRKLKLACGAYHAGLDIKSRREIHHRFMRDEIQCVVATIAFGMGINKADIRKVIHYGAPKEMESYYQEIGRAGRDGLQSSCHVLWAPADICLNRHRLSEITSEKFRLYKLKMMAKMEKYLHSTRCRRQLILSHFEDKQLRKASVGIMGTEQCCDNCRSRLDQCSSIDDSDDASQDFGPQTFQLLSAVKILGEKFGIGLPILFLQGSNSQRLEDKYRRHSLFGTGKDQKESWWKALSRQLMAEGFLVEVPGRQKFIRICTLTEKGRCWLHEANREFPKKLKLEANEELCPRVFLLPSSKTVPPGAEQHSSNRVPIELTAEKKSNLEKMYSYKASDKISSGSNIPEKSIIVHSPGKSYKSSEPVISAEEQETQTVLYGILVEARQKHAGKMDVPPAILATNKILLEMAKMRPTTFENMKRINGVSEGKATMLTPLLEVIKHFCQINNVQSDLFSSTKPWEEPRNSVVEENETCSLSQSAAITYSLFQEKKMSLISLTEDRIMPLTAVGMHLTQAVKAGCPLDMERAGLTPEVQKIITDVIRNPPINSDINKIKLIRKLVPENIDTYLIHMVIEILEKDSDNRLLSDGLSCASNRKRYFPNSEENCSSSKRSREEVGTNTKVDGKHPYDFSLKDESHKNSLRLTSWNQPTSDSETDLFTDSHPQTSSITSKRKLPKWFAKDTETLADTSRKSAAKAQKKGLFS

Target ORF information:

RefSeq Version	XM_014120074.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris Werner syndrome, RecQ helicase-like (WRN), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120074.1

ORF Insert Sequence:

ATGTTGCTTG AAAATGAAGC AATTAAAAAA GCAGGTGTAG GAATCAAAGG AGATCAGCGG 
AAACTTCTAT GTGACTTTGA CATCGATTTG AAGAATTTTG TGGAGCTGAC AGATGTTGCC 
AATGAAAAGC TGAAATGTAC AGAGACTTGG AGCCTCAATG GTTTGGTTAA ACACCTCTTT 
GGCAAACAGC TTCTGAAAGA CAGATCTATC CGTTGTAGCA ATTGGGGTGA TTTTCCTCTC 
ACTGAGGATC AGAAACTGTA TGCAGCCACT GATGCGTATG CTGGTCTCAT CATTTATCAG 
AAATTAGAGA TTTTAGGTAA TGCTGTGGAA AGGTTTGCTA TAAATAAAAA AGAGGAGGAA 
ATCCTACCTA TTGATGTGAA GAAACAATTG ACTTCAATCT CTGAGGAGAT GATGGATCTG 
GCCAAGCATC TTCCTGACGC TTTGAAAAAA TTAGAAGATC CACAGAGGGT TTCTCTACTA 
TTAAGTGCTA TTTCAGAAAA TCTGTATTCA TTGAGGAAGA TGATAATTGG TTCTCCTGAG 
GCCGAATTGA GGCACAGCAC TAATGTTCAC TTATTATCAT TGGGAGATCC AACTGCTGAT 
GAAGTACAGC AGAAACAAAT TAGAGAACAT AAATTTTTTC CTAAAGTTAA AGATGAGACT 
TGGGACACAA CATTTGATAA TTTAATTAAA CATGATGAAG GAACCGAACC TCAAAACAAA 
GTGAAACAAA AAGAAGATGG ACTTGAAGAT GGATTAGAAG TTGGCAATAA ATTGAAAACG 
AACACAGAAA GAATTTGTTT GATGTCATTA GATATAACAG AACACGAACT CCAGATTTTG 
GAACAGCAGG CTCAGGAAAA ATTTCTTAAT GATGTCACTT GCAAATCTCC TGAGCATTTA 
TCTTCCAAAG ATAATGAAGA GGATCTATCT TATGTAATTG AAAGCGATGA AGATTTAGAA 
AAGGAAATGA TTAAGTCTTT AGAAAATTTA AATACTGGCA TGGCAGATCC AATTCATTCA 
AAATGCATAA AAATGGAAGG AAATCTGGAT GTTCCTTTTG AAGATGATGA AGATGAGATT 
GAAGCTATTG AAGAAGAAGA TGATAATGTT GAAGACTATT CATTACCAAG ACCTAATGCA 
AGGCAAATTA ATTTCCTCAA GATCTATTTT GGCCATTCCA GTTTTAAACC GGTTCAGTGG 
AAGGTGATTC ATTCTGTATT GGAAGAAAGG AGAGATAATG TTGTTGTGAT GGCAACTGGA 
TATGGAAAGA GTTTGTGCTT CCAGTACCCA CCTGTGTATT CAGGGAGGAT TGGTCTTGTC 
ATCTCTCCTC TTATTTCTTT GATGGAAGAC CAAGTGCTCC AGCTTAAGAT GTCCAATATT 
CCAGCTTGTT TTCTGGGATC AGCACAGTCA AAAAATGTTC TAGAAGATAT TAAATTAGGC 
AAATATCGGA TTGTATACAT AACTCCAGAA TTCTGTTCTG GTAACTTGTT CCTACTCCAG 
CAACTTCAGG CTGATATTGG TATCACACTT ATTGCTGTGG ATGAGGCTCA CTGTATTTCT 
GAATGGGGAC ATGATTTTAG AAGTTCATTC AGGACTTTGG GCTCCCTAAA GGCAATACTC 
CCATCGGTTC CAATTGTTGC ACTTACTGCT ACTGCAAGTT CTTCAATCCG GGAAGACATT 
GTACGCTGCT TAAACCTGAA GAATCCTCAG ATTACCTGTA CTGGTTTTGA TCGACCAAAC 
TTGTATTTAG AAGTTAGGCG AAAAACAGGA AGCATCCTTC AGGATCTTGA CCAATTTCTT 
GTCCAAAAGA CAAGTTCCAT CTGGGAATTT GAAGGTCCAA CTATCATCTA TTGTCCTTCC 
CGAAAAACAA CAGAACAAGT TACAGCTGAA CTTAGGAAAC TGAAGTTAGC CTGTGGAGCA 
TACCATGCAG GCTTGGATAT TAAATCAAGG AGAGAAATTC ATCATAGGTT CATGAGAGAT 
GAAATTCAGT GTGTTGTAGC TACCATAGCT TTTGGAATGG GCATTAATAA AGCTGACATT 
CGCAAAGTCA TTCATTATGG TGCTCCTAAG GAAATGGAAT CATATTACCA GGAGATTGGG 
AGAGCTGGCC GTGATGGACT TCAGAGTTCT TGTCATGTAC TCTGGGCTCC AGCAGACATT 
TGCTTAAATA GGCACCGCCT TAGTGAGATA ACCAGTGAAA AGTTTCGATT ATACAAATTA 
AAGATGATGG CGAAGATGGA AAAATATCTT CATTCCACAA GATGTAGGCG ACAACTCATC 
TTGTCTCATT TTGAAGACAA ACAACTACGA AAGGCCTCCG TGGGTATTAT GGGAACTGAA 
CAATGCTGTG ATAATTGCAG ATCCAGATTG GATCAATGCT CTTCCATTGA TGATTCAGAT 
GACGCATCAC AGGATTTTGG TCCACAAACA TTCCAGCTAT TATCTGCTGT GAAAATCTTA 
GGAGAAAAGT TTGGAATTGG GCTTCCGATT TTATTTCTCC AAGGATCTAA TTCCCAGCGT 
CTTGAAGATA AGTATCGCAG ACACAGTTTA TTTGGCACTG GCAAGGATCA AAAAGAGAGT 
TGGTGGAAGG CTTTGTCCCG TCAGCTCATG GCTGAAGGAT TCTTGGTAGA AGTTCCTGGG 
AGACAAAAAT TTATAAGGAT TTGCACTCTT ACAGAAAAGG GCAGATGTTG GCTTCATGAA 
GCCAATAGAG AATTTCCTAA GAAACTTAAA CTTGAAGCTA ATGAAGAATT GTGTCCGAGG 
GTGTTTCTTC TACCAAGTTC TAAAACTGTA CCACCTGGCG CTGAACAACA TTCCTCTAAT 
CGAGTACCAA TTGAATTAAC TGCAGAGAAG AAGTCTAACT TGGAGAAGAT GTATTCTTAC 
AAAGCATCTG ATAAAATTTC TTCTGGGAGT AACATTCCTG AAAAAAGCAT CATAGTACAC 
TCACCAGGAA AATCTTACAA GTCTTCAGAA CCTGTTATTT CAGCTGAAGA GCAGGAGACT 
CAGACTGTGT TGTATGGCAT ATTGGTGGAA GCCAGGCAGA AACATGCCGG TAAAATGGAT 
GTTCCTCCAG CCATTCTAGC AACAAATAAG ATACTATTGG AAATGGCCAA AATGAGACCT 
ACTACATTTG AAAATATGAA AAGGATCAAT GGTGTGTCTG AAGGCAAAGC TACTATGCTG 
ACCCCTCTGT TGGAAGTCAT CAAACATTTC TGTCAGATAA ATAATGTTCA GTCAGACCTC 
TTTTCAAGTA CAAAACCTTG GGAAGAACCA AGGAACAGTG TGGTGGAGGA AAATGAAACA 
TGTTCCCTTT CACAGTCTGC GGCCATCACG TATTCCTTAT TCCAAGAAAA GAAGATGTCT 
TTGATAAGTT TAACTGAGGA CAGGATTATG CCTCTCACAG CAGTTGGAAT GCACTTAACC 
CAAGCAGTGA AAGCTGGCTG TCCACTTGAT ATGGAGCGAG CAGGCCTGAC TCCAGAGGTT 
CAGAAGATTA TTACTGATGT TATCCGAAAC CCTCCCATCA ACTCAGATAT AAATAAAATT 
AAACTAATTA GAAAGCTGGT TCCTGAAAAC ATTGACACGT ACCTTATCCA CATGGTAATT 
GAGATCCTGG AAAAAGACTC TGACAACAGA CTGTTGTCAG ACGGGCTTTC ATGTGCTTCC 
AACAGAAAGA GATATTTCCC CAATTCTGAA GAGAACTGTT CAAGTTCTAA AAGAAGTAGG 
GAAGAAGTAG GCACTAATAC CAAGGTTGAT GGAAAACATC CTTACGATTT TTCATTGAAA 
GATGAAAGTC ACAAAAATTC CCTAAGACTA ACGTCTTGGA ATCAACCAAC CAGCGATTCA 
GAAACAGATT TATTTACAGA TTCTCACCCA CAGACTTCAT CTATAACCTC CAAGAGAAAA 
TTACCTAAGT GGTTTGCCAA AGACACTGAG ACTTTAGCTG ACACCAGCAG GAAATCAGCA 
GCCAAAGCAC AAAAGAAAGG TCTTTTTAGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WRN ( XM_014120074.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013975549.1 Canis lupus familiaris Werner syndrome RecQ like helicase (WRN), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe40575
Clone ID Related Accession (Same CDS sequence)	XM_014120074.1 , XM_014120074.2
Accession Version	XM_014120074.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	Werner syndrome ATP-dependent helicase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014120074.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCTTG AAAATGAAGC AATTAAAAAA GCAGGTGTAG GAATCAAAGG AGATCAGCGG 
AAACTTCTAT GTGACTTTGA CATCGATTTG AAGAATTTTG TGGAGCTGAC AGATGTTGCC 
AATGAAAAGC TGAAATGTAC AGAGACTTGG AGCCTCAATG GTTTGGTTAA ACACCTCTTT 
GGCAAACAGC TTCTGAAAGA CAGATCTATC CGTTGTAGCA ATTGGGGTGA TTTTCCTCTC 
ACTGAGGATC AGAAACTGTA TGCAGCCACT GATGCGTATG CTGGTCTCAT CATTTATCAG 
AAATTAGAGA TTTTAGGTAA TGCTGTGGAA AGGTTTGCTA TAAATAAAAA AGAGGAGGAA 
ATCCTACCTA TTGATGTGAA GAAACAATTG ACTTCAATCT CTGAGGAGAT GATGGATCTG 
GCCAAGCATC TTCCTGACGC TTTGAAAAAA TTAGAAGATC CACAGAGGGT TTCTCTACTA 
TTAAGTGCTA TTTCAGAAAA TCTGTATTCA TTGAGGAAGA TGATAATTGG TTCTCCTGAG 
GCCGAATTGA GGCACAGCAC TAATGTTCAC TTATTATCAT TGGGAGATCC AACTGCTGAT 
GAAGTACAGC AGAAACAAAT TAGAGAACAT AAATTTTTTC CTAAAGTTAA AGATGAGACT 
TGGGACACAA CATTTGATAA TTTAATTAAA CATGATGAAG GAACCGAACC TCAAAACAAA 
GTGAAACAAA AAGAAGATGG ACTTGAAGAT GGATTAGAAG TTGGCAATAA ATTGAAAACG 
AACACAGAAA GAATTTGTTT GATGTCATTA GATATAACAG AACACGAACT CCAGATTTTG 
GAACAGCAGG CTCAGGAAAA ATTTCTTAAT GATGTCACTT GCAAATCTCC TGAGCATTTA 
TCTTCCAAAG ATAATGAAGA GGATCTATCT TATGTAATTG AAAGCGATGA AGATTTAGAA 
AAGGAAATGA TTAAGTCTTT AGAAAATTTA AATACTGGCA TGGCAGATCC AATTCATTCA 
AAATGCATAA AAATGGAAGG AAATCTGGAT GTTCCTTTTG AAGATGATGA AGATGAGATT 
GAAGCTATTG AAGAAGAAGA TGATAATGTT GAAGACTATT CATTACCAAG ACCTAATGCA 
AGGCAAATTA ATTTCCTCAA GATCTATTTT GGCCATTCCA GTTTTAAACC GGTTCAGTGG 
AAGGTGATTC ATTCTGTATT GGAAGAAAGG AGAGATAATG TTGTTGTGAT GGCAACTGGA 
TATGGAAAGA GTTTGTGCTT CCAGTACCCA CCTGTGTATT CAGGGAGGAT TGGTCTTGTC 
ATCTCTCCTC TTATTTCTTT GATGGAAGAC CAAGTGCTCC AGCTTAAGAT GTCCAATATT 
CCAGCTTGTT TTCTGGGATC AGCACAGTCA AAAAATGTTC TAGAAGATAT TAAATTAGGC 
AAATATCGGA TTGTATACAT AACTCCAGAA TTCTGTTCTG GTAACTTGTT CCTACTCCAG 
CAACTTCAGG CTGATATTGG TATCACACTT ATTGCTGTGG ATGAGGCTCA CTGTATTTCT 
GAATGGGGAC ATGATTTTAG AAGTTCATTC AGGACTTTGG GCTCCCTAAA GGCAATACTC 
CCATCGGTTC CAATTGTTGC ACTTACTGCT ACTGCAAGTT CTTCAATCCG GGAAGACATT 
GTACGCTGCT TAAACCTGAA GAATCCTCAG ATTACCTGTA CTGGTTTTGA TCGACCAAAC 
TTGTATTTAG AAGTTAGGCG AAAAACAGGA AGCATCCTTC AGGATCTTGA CCAATTTCTT 
GTCCAAAAGA CAAGTTCCAT CTGGGAATTT GAAGGTCCAA CTATCATCTA TTGTCCTTCC 
CGAAAAACAA CAGAACAAGT TACAGCTGAA CTTAGGAAAC TGAAGTTAGC CTGTGGAGCA 
TACCATGCAG GCTTGGATAT TAAATCAAGG AGAGAAATTC ATCATAGGTT CATGAGAGAT 
GAAATTCAGT GTGTTGTAGC TACCATAGCT TTTGGAATGG GCATTAATAA AGCTGACATT 
CGCAAAGTCA TTCATTATGG TGCTCCTAAG GAAATGGAAT CATATTACCA GGAGATTGGG 
AGAGCTGGCC GTGATGGACT TCAGAGTTCT TGTCATGTAC TCTGGGCTCC AGCAGACATT 
TGCTTAAATA GGCACCGCCT TAGTGAGATA ACCAGTGAAA AGTTTCGATT ATACAAATTA 
AAGATGATGG CGAAGATGGA AAAATATCTT CATTCCACAA GATGTAGGCG ACAACTCATC 
TTGTCTCATT TTGAAGACAA ACAACTACGA AAGGCCTCCG TGGGTATTAT GGGAACTGAA 
CAATGCTGTG ATAATTGCAG ATCCAGATTG GATCAATGCT CTTCCATTGA TGATTCAGAT 
GACGCATCAC AGGATTTTGG TCCACAAACA TTCCAGCTAT TATCTGCTGT GAAAATCTTA 
GGAGAAAAGT TTGGAATTGG GCTTCCGATT TTATTTCTCC AAGGATCTAA TTCCCAGCGT 
CTTGAAGATA AGTATCGCAG ACACAGTTTA TTTGGCACTG GCAAGGATCA AAAAGAGAGT 
TGGTGGAAGG CTTTGTCCCG TCAGCTCATG GCTGAAGGAT TCTTGGTAGA AGTTCCTGGG 
AGACAAAAAT TTATAAGGAT TTGCACTCTT ACAGAAAAGG GCAGATGTTG GCTTCATGAA 
GCCAATAGAG AATTTCCTAA GAAACTTAAA CTTGAAGCTA ATGAAGAATT GTGTCCGAGG 
GTGTTTCTTC TACCAAGTTC TAAAACTGTA CCACCTGGCG CTGAACAACA TTCCTCTAAT 
CGAGTACCAA TTGAATTAAC TGCAGAGAAG AAGTCTAACT TGGAGAAGAT GTATTCTTAC 
AAAGCATCTG ATAAAATTTC TTCTGGGAGT AACATTCCTG AAAAAAGCAT CATAGTACAC 
TCACCAGGAA AATCTTACAA GTCTTCAGAA CCTGTTATTT CAGCTGAAGA GCAGGAGACT 
CAGACTGTGT TGTATGGCAT ATTGGTGGAA GCCAGGCAGA AACATGCCGG TAAAATGGAT 
GTTCCTCCAG CCATTCTAGC AACAAATAAG ATACTATTGG AAATGGCCAA AATGAGACCT 
ACTACATTTG AAAATATGAA AAGGATCAAT GGTGTGTCTG AAGGCAAAGC TACTATGCTG 
ACCCCTCTGT TGGAAGTCAT CAAACATTTC TGTCAGATAA ATAATGTTCA GTCAGACCTC 
TTTTCAAGTA CAAAACCTTG GGAAGAACCA AGGAACAGTG TGGTGGAGGA AAATGAAACA 
TGTTCCCTTT CACAGTCTGC GGCCATCACG TATTCCTTAT TCCAAGAAAA GAAGATGTCT 
TTGATAAGTT TAACTGAGGA CAGGATTATG CCTCTCACAG CAGTTGGAAT GCACTTAACC 
CAAGCAGTGA AAGCTGGCTG TCCACTTGAT ATGGAGCGAG CAGGCCTGAC TCCAGAGGTT 
CAGAAGATTA TTACTGATGT TATCCGAAAC CCTCCCATCA ACTCAGATAT AAATAAAATT 
AAACTAATTA GAAAGCTGGT TCCTGAAAAC ATTGACACGT ACCTTATCCA CATGGTAATT 
GAGATCCTGG AAAAAGACTC TGACAACAGA CTGTTGTCAG ACGGGCTTTC ATGTGCTTCC 
AACAGAAAGA GATATTTCCC CAATTCTGAA GAGAACTGTT CAAGTTCTAA AAGAAGTAGG 
GAAGAAGTAG GCACTAATAC CAAGGTTGAT GGAAAACATC CTTACGATTT TTCATTGAAA 
GATGAAAGTC ACAAAAATTC CCTAAGACTA ACGTCTTGGA ATCAACCAAC CAGCGATTCA 
GAAACAGATT TATTTACAGA TTCTCACCCA CAGACTTCAT CTATAACCTC CAAGAGAAAA 
TTACCTAAGT GGTTTGCCAA AGACACTGAG ACTTTAGCTG ACACCAGCAG GAAATCAGCA 
GCCAAAGCAC AAAAGAAAGG TCTTTTTAGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013975549.1
CDS	147..4139
Translation	MLLENEAIKKAGVGIKGDQRKLLCDFDIDLKNFVELTDVANEKLKCTETWSLNGLVKHLFGKQLLKDRSIRCSNWGDFPLTEDQKLYAATDAYAGLIIYQKLEILGNAVERFAINKKEEEILPIDVKKQLTSISEEMMDLAKHLPDALKKLEDPQRVSLLLSAISENLYSLRKMIIGSPEAELRHSTNVHLLSLGDPTADEVQQKQIREHKFFPKVKDETWDTTFDNLIKHDEGTEPQNKVKQKEDGLEDGLEVGNKLKTNTERICLMSLDITEHELQILEQQAQEKFLNDVTCKSPEHLSSKDNEEDLSYVIESDEDLEKEMIKSLENLNTGMADPIHSKCIKMEGNLDVPFEDDEDEIEAIEEEDDNVEDYSLPRPNARQINFLKIYFGHSSFKPVQWKVIHSVLEERRDNVVVMATGYGKSLCFQYPPVYSGRIGLVISPLISLMEDQVLQLKMSNIPACFLGSAQSKNVLEDIKLGKYRIVYITPEFCSGNLFLLQQLQADIGITLIAVDEAHCISEWGHDFRSSFRTLGSLKAILPSVPIVALTATASSSIREDIVRCLNLKNPQITCTGFDRPNLYLEVRRKTGSILQDLDQFLVQKTSSIWEFEGPTIIYCPSRKTTEQVTAELRKLKLACGAYHAGLDIKSRREIHHRFMRDEIQCVVATIAFGMGINKADIRKVIHYGAPKEMESYYQEIGRAGRDGLQSSCHVLWAPADICLNRHRLSEITSEKFRLYKLKMMAKMEKYLHSTRCRRQLILSHFEDKQLRKASVGIMGTEQCCDNCRSRLDQCSSIDDSDDASQDFGPQTFQLLSAVKILGEKFGIGLPILFLQGSNSQRLEDKYRRHSLFGTGKDQKESWWKALSRQLMAEGFLVEVPGRQKFIRICTLTEKGRCWLHEANREFPKKLKLEANEELCPRVFLLPSSKTVPPGAEQHSSNRVPIELTAEKKSNLEKMYSYKASDKISSGSNIPEKSIIVHSPGKSYKSSEPVISAEEQETQTVLYGILVEARQKHAGKMDVPPAILATNKILLEMAKMRPTTFENMKRINGVSEGKATMLTPLLEVIKHFCQINNVQSDLFSSTKPWEEPRNSVVEENETCSLSQSAAITYSLFQEKKMSLISLTEDRIMPLTAVGMHLTQAVKAGCPLDMERAGLTPEVQKIITDVIRNPPINSDINKIKLIRKLVPENIDTYLIHMVIEILEKDSDNRLLSDGLSCASNRKRYFPNSEENCSSSKRSREEVGTNTKVDGKHPYDFSLKDESHKNSLRLTSWNQPTSDSETDLFTDSHPQTSSITSKRKLPKWFAKDTETLADTSRKSAAKAQKKGLFS

Target ORF information:

RefSeq Version	XM_014120074.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris Werner syndrome RecQ like helicase (WRN), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120074.2

ORF Insert Sequence:

ATGTTGCTTG AAAATGAAGC AATTAAAAAA GCAGGTGTAG GAATCAAAGG AGATCAGCGG 
AAACTTCTAT GTGACTTTGA CATCGATTTG AAGAATTTTG TGGAGCTGAC AGATGTTGCC 
AATGAAAAGC TGAAATGTAC AGAGACTTGG AGCCTCAATG GTTTGGTTAA ACACCTCTTT 
GGCAAACAGC TTCTGAAAGA CAGATCTATC CGTTGTAGCA ATTGGGGTGA TTTTCCTCTC 
ACTGAGGATC AGAAACTGTA TGCAGCCACT GATGCGTATG CTGGTCTCAT CATTTATCAG 
AAATTAGAGA TTTTAGGTAA TGCTGTGGAA AGGTTTGCTA TAAATAAAAA AGAGGAGGAA 
ATCCTACCTA TTGATGTGAA GAAACAATTG ACTTCAATCT CTGAGGAGAT GATGGATCTG 
GCCAAGCATC TTCCTGACGC TTTGAAAAAA TTAGAAGATC CACAGAGGGT TTCTCTACTA 
TTAAGTGCTA TTTCAGAAAA TCTGTATTCA TTGAGGAAGA TGATAATTGG TTCTCCTGAG 
GCCGAATTGA GGCACAGCAC TAATGTTCAC TTATTATCAT TGGGAGATCC AACTGCTGAT 
GAAGTACAGC AGAAACAAAT TAGAGAACAT AAATTTTTTC CTAAAGTTAA AGATGAGACT 
TGGGACACAA CATTTGATAA TTTAATTAAA CATGATGAAG GAACCGAACC TCAAAACAAA 
GTGAAACAAA AAGAAGATGG ACTTGAAGAT GGATTAGAAG TTGGCAATAA ATTGAAAACG 
AACACAGAAA GAATTTGTTT GATGTCATTA GATATAACAG AACACGAACT CCAGATTTTG 
GAACAGCAGG CTCAGGAAAA ATTTCTTAAT GATGTCACTT GCAAATCTCC TGAGCATTTA 
TCTTCCAAAG ATAATGAAGA GGATCTATCT TATGTAATTG AAAGCGATGA AGATTTAGAA 
AAGGAAATGA TTAAGTCTTT AGAAAATTTA AATACTGGCA TGGCAGATCC AATTCATTCA 
AAATGCATAA AAATGGAAGG AAATCTGGAT GTTCCTTTTG AAGATGATGA AGATGAGATT 
GAAGCTATTG AAGAAGAAGA TGATAATGTT GAAGACTATT CATTACCAAG ACCTAATGCA 
AGGCAAATTA ATTTCCTCAA GATCTATTTT GGCCATTCCA GTTTTAAACC GGTTCAGTGG 
AAGGTGATTC ATTCTGTATT GGAAGAAAGG AGAGATAATG TTGTTGTGAT GGCAACTGGA 
TATGGAAAGA GTTTGTGCTT CCAGTACCCA CCTGTGTATT CAGGGAGGAT TGGTCTTGTC 
ATCTCTCCTC TTATTTCTTT GATGGAAGAC CAAGTGCTCC AGCTTAAGAT GTCCAATATT 
CCAGCTTGTT TTCTGGGATC AGCACAGTCA AAAAATGTTC TAGAAGATAT TAAATTAGGC 
AAATATCGGA TTGTATACAT AACTCCAGAA TTCTGTTCTG GTAACTTGTT CCTACTCCAG 
CAACTTCAGG CTGATATTGG TATCACACTT ATTGCTGTGG ATGAGGCTCA CTGTATTTCT 
GAATGGGGAC ATGATTTTAG AAGTTCATTC AGGACTTTGG GCTCCCTAAA GGCAATACTC 
CCATCGGTTC CAATTGTTGC ACTTACTGCT ACTGCAAGTT CTTCAATCCG GGAAGACATT 
GTACGCTGCT TAAACCTGAA GAATCCTCAG ATTACCTGTA CTGGTTTTGA TCGACCAAAC 
TTGTATTTAG AAGTTAGGCG AAAAACAGGA AGCATCCTTC AGGATCTTGA CCAATTTCTT 
GTCCAAAAGA CAAGTTCCAT CTGGGAATTT GAAGGTCCAA CTATCATCTA TTGTCCTTCC 
CGAAAAACAA CAGAACAAGT TACAGCTGAA CTTAGGAAAC TGAAGTTAGC CTGTGGAGCA 
TACCATGCAG GCTTGGATAT TAAATCAAGG AGAGAAATTC ATCATAGGTT CATGAGAGAT 
GAAATTCAGT GTGTTGTAGC TACCATAGCT TTTGGAATGG GCATTAATAA AGCTGACATT 
CGCAAAGTCA TTCATTATGG TGCTCCTAAG GAAATGGAAT CATATTACCA GGAGATTGGG 
AGAGCTGGCC GTGATGGACT TCAGAGTTCT TGTCATGTAC TCTGGGCTCC AGCAGACATT 
TGCTTAAATA GGCACCGCCT TAGTGAGATA ACCAGTGAAA AGTTTCGATT ATACAAATTA 
AAGATGATGG CGAAGATGGA AAAATATCTT CATTCCACAA GATGTAGGCG ACAACTCATC 
TTGTCTCATT TTGAAGACAA ACAACTACGA AAGGCCTCCG TGGGTATTAT GGGAACTGAA 
CAATGCTGTG ATAATTGCAG ATCCAGATTG GATCAATGCT CTTCCATTGA TGATTCAGAT 
GACGCATCAC AGGATTTTGG TCCACAAACA TTCCAGCTAT TATCTGCTGT GAAAATCTTA 
GGAGAAAAGT TTGGAATTGG GCTTCCGATT TTATTTCTCC AAGGATCTAA TTCCCAGCGT 
CTTGAAGATA AGTATCGCAG ACACAGTTTA TTTGGCACTG GCAAGGATCA AAAAGAGAGT 
TGGTGGAAGG CTTTGTCCCG TCAGCTCATG GCTGAAGGAT TCTTGGTAGA AGTTCCTGGG 
AGACAAAAAT TTATAAGGAT TTGCACTCTT ACAGAAAAGG GCAGATGTTG GCTTCATGAA 
GCCAATAGAG AATTTCCTAA GAAACTTAAA CTTGAAGCTA ATGAAGAATT GTGTCCGAGG 
GTGTTTCTTC TACCAAGTTC TAAAACTGTA CCACCTGGCG CTGAACAACA TTCCTCTAAT 
CGAGTACCAA TTGAATTAAC TGCAGAGAAG AAGTCTAACT TGGAGAAGAT GTATTCTTAC 
AAAGCATCTG ATAAAATTTC TTCTGGGAGT AACATTCCTG AAAAAAGCAT CATAGTACAC 
TCACCAGGAA AATCTTACAA GTCTTCAGAA CCTGTTATTT CAGCTGAAGA GCAGGAGACT 
CAGACTGTGT TGTATGGCAT ATTGGTGGAA GCCAGGCAGA AACATGCCGG TAAAATGGAT 
GTTCCTCCAG CCATTCTAGC AACAAATAAG ATACTATTGG AAATGGCCAA AATGAGACCT 
ACTACATTTG AAAATATGAA AAGGATCAAT GGTGTGTCTG AAGGCAAAGC TACTATGCTG 
ACCCCTCTGT TGGAAGTCAT CAAACATTTC TGTCAGATAA ATAATGTTCA GTCAGACCTC 
TTTTCAAGTA CAAAACCTTG GGAAGAACCA AGGAACAGTG TGGTGGAGGA AAATGAAACA 
TGTTCCCTTT CACAGTCTGC GGCCATCACG TATTCCTTAT TCCAAGAAAA GAAGATGTCT 
TTGATAAGTT TAACTGAGGA CAGGATTATG CCTCTCACAG CAGTTGGAAT GCACTTAACC 
CAAGCAGTGA AAGCTGGCTG TCCACTTGAT ATGGAGCGAG CAGGCCTGAC TCCAGAGGTT 
CAGAAGATTA TTACTGATGT TATCCGAAAC CCTCCCATCA ACTCAGATAT AAATAAAATT 
AAACTAATTA GAAAGCTGGT TCCTGAAAAC ATTGACACGT ACCTTATCCA CATGGTAATT 
GAGATCCTGG AAAAAGACTC TGACAACAGA CTGTTGTCAG ACGGGCTTTC ATGTGCTTCC 
AACAGAAAGA GATATTTCCC CAATTCTGAA GAGAACTGTT CAAGTTCTAA AAGAAGTAGG 
GAAGAAGTAG GCACTAATAC CAAGGTTGAT GGAAAACATC CTTACGATTT TTCATTGAAA 
GATGAAAGTC ACAAAAATTC CCTAAGACTA ACGTCTTGGA ATCAACCAAC CAGCGATTCA 
GAAACAGATT TATTTACAGA TTCTCACCCA CAGACTTCAT CTATAACCTC CAAGAGAAAA 
TTACCTAAGT GGTTTGCCAA AGACACTGAG ACTTTAGCTG ACACCAGCAG GAAATCAGCA 
GCCAAAGCAC AAAAGAAAGG TCTTTTTAGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WRN ( XM_014120075.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013975550.1 Canis lupus familiaris Werner syndrome, RecQ helicase-like (WRN), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe40576
Clone ID Related Accession (Same CDS sequence)	XM_014120075.1
Accession Version	XM_014120075.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	Werner syndrome ATP-dependent helicase isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726087.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCTAT TAAAGATGAA TGAAAAAAAA TTGGAAAGAA CTCCACAGCC GCGAAAACTT 
CCTGAATGGA TGTCTATGCA GAATGAGACA TGTACTGCAG TAGAAACAAA GGCATCGATT 
CAGAAGAGTG TTTTTGAAGA CGACCTCTCT TTCTTAGAAT TCACTGGATC TATTGTTTAT 
AGTTATGAAG CTAGTGATTG TTCTTTTCTA TCAGAAGATA TTAGCATGAA TCTGTCTACT 
GGAGGTGTGG TGGGTTTTGA CATGGAATGG CCACCAGTAT ACACTAAAAG GAAACCGAGC 
CGAGTTGCAC TGATTCAGTT ATGTGTGTCA GAGAACAAAT GTTACTTGTT TCATATTTCT 
TCTATGTCAG TTTTTCCTCA GGGGTTAAAA ATGTTGCTTG AAAATGAAGC AATTAAAAAA 
GCAGGTGTAG GAATCAAAGG AGATCAGCGG AAACTTCTAT GTGACTTTGA CATCGATTTG 
AAGAATTTTG TGGAGCTGAC AGATGTTGCC AATGAAAAGC TGAAATGTAC AGAGACTTGG 
AGCCTCAATG GTTTGGTTAA ACACCTCTTT GGCAAACAGC TTCTGAAAGA CAGATCTATC 
CGTTGTAGCA ATTGGGGTGA TTTTCCTCTC ACTGAGGATC AGAAACTGTA TGCAGCCACT 
GATGCGTATG CTGGTCTCAT CATTTATCAG AAATTAGAGA TTTTAGGTAA TGCTGTGGAA 
AGGTTTGCTA TAAATAAAAA AGAGGAGGAA ATCCTACCTA TTGATGTGAA GAAACAATTG 
ACTTCAATCT CTGAGGAGAT GATGGATCTG GCCAAGCATC TTCCTGACGC TTTGAAAAAA 
TTAGAAGATC CACAGAGGGT TTCTCTACTA TTAAGTGCTA TTTCAGAAAA TCTGTATTCA 
TTGAGGAAGA TGATAATTGG TTCTCCTGAG GCCGAATTGA GGCACAGCAC TAATGTTCAC 
TTATTATCAT TGGGAGATCC AACTGCTGAT GAAGTACAGC AGAAACAAAT TAGAGAACAT 
AAATTTTTTC CTAAAGTTAA AGATGAGACT TGGGACACAA CATTTGATAA TTTAATTAAA 
CATGATGAAG GAACCGAACC TCAAAACAAA GTGAAACAAA AAGAAGATGG ACTTGAAGAT 
GGATTAGAAG TTGGCAATAA ATTGAAAACG AACACAGAAA GAATTTGTTT GATGTCATTA 
GATATAACAG AACACGAACT CCAGATTTTG GAACAGCAGG CTCAGGAAAA ATTTCTTAAT 
GATGTCACTT GCAAATCTCC TGAGCATTTA TCTTCCAAAG ATAATGAAGA GGATCTATCT 
TATGTAATTG AAAGCGATGA AGATTTAGAA AAGGAAATGA TTAAGTCTTT AGAAAATTTA 
AATACTGGCA TGGCAGATCC AATTCATTCA AAATGCATAA AAATGGAAGG AAATCTGGAT 
GTTCCTTTTG AAGATGATGA AGATGAGATT GAAGCTATTG AAGAAGAAGA TGATAATGTT 
GAAGACTATT CATTACCAAG ACCTAATGCA AGGCAAATTA ATTTCCTCAA GATCTATTTT 
GGCCATTCCA GTTTTAAACC GGTTCAGTGG AAGGTGATTC ATTCTGTATT GGAAGAAAGG 
AGAGATAATG TTGTTGTGAT GGCAACTGGA TATGGAAAGA GTTTGTGCTT CCAGTACCCA 
CCTGTGTATT CAGGGAGGAT TGGTCTTGTC ATCTCTCCTC TTATTTCTTT GATGGAAGAC 
CAAGTGCTCC AGCTTAAGAT GTCCAATATT CCAGCTTGTT TTCTGGGATC AGCACAGTCA 
AAAAATGTTC TAGAAGATAT TAAATTAGGC AAATATCGGA TTGTATACAT AACTCCAGAA 
TTCTGTTCTG GTAACTTGTT CCTACTCCAG CAACTTCAGG CTGATATTGG TATCACACTT 
ATTGCTGTGG ATGAGGCTCA CTGTATTTCT GAATGGGGAC ATGATTTTAG AAGTTCATTC 
AGGACTTTGG GCTCCCTAAA GGCAATACTC CCATCGGTTC CAATTGTTGC ACTTACTGCT 
ACTGCAAGTT CTTCAATCCG GGAAGACATT GTACGCTGCT TAAACCTGAA GAATCCTCAG 
ATTACCTGTA CTGGTTTTGA TCGACCAAAC TTGTATTTAG AAGTTAGGCG AAAAACAGGA 
AGCATCCTTC AGGATCTTGA CCAATTTCTT GTCCAAAAGA CAAGTTCCAT CTGGGAATTT 
GAAGGTCCAA CTATCATCTA TTGTCCTTCC CGAAAAACAA CAGAACAAGT TACAGCTGAA 
CTTAGGAAAC TGAAGTTAGC CTGTGGAGCA TACCATGCAG GCTTGGATAT TAAATCAAGG 
AGAGAAATTC ATCATAGGTT CATGAGAGAT GAAATTCAGT GTGTTGTAGC TACCATAGCT 
TTTGGAATGG GCATTAATAA AGCTGACATT CGCAAAGTCA TTCATTATGG TGCTCCTAAG 
GAAATGGAAT CATATTACCA GGAGATTGGG AGAGCTGGCC GTGATGGACT TCAGAGTTCT 
TGTCATGTAC TCTGGGCTCC AGCAGACATT TGCTTAAATA GGCACCGCCT TAGTGAGATA 
ACCAGTGAAA AGTTTCGATT ATACAAATTA AAGATGATGG CGAAGATGGA AAAATATCTT 
CATTCCACAA GATGTAGGCG ACAACTCATC TTGTCTCATT TTGAAGACAA ACAACTACGA 
AAGGCCTCCG TGGGTATTAT GGGAACTGAA CAATGCTGTG ATAATTGCAG ATCCAGATTG 
GATCAATGCT CTTCCATTGA TGATTCAGAT GACGCATCAC AGGATTTTGG TCCACAAACA 
TTCCAGCTAT TATCTGCTGT GAAAATCTTA GGAGAAAAGT TTGGAATTGG GCTTCCGATT 
TTATTTCTCC AAGGATCTAA TTCCCAGCGT CTTGAAGATA AGTATCGCAG ACACAGTTTA 
TTTGGCACTG GCAAGGATCA AAAAGAGAGT TGGTGGAAGG CTTTGTCCCG TCAGCTCATG 
GCTGAAGGAT TCTTGGTAGA AGTTCCTGGG AGACAAAAAT TTATAAGGAT TTGCACTCTT 
ACAGAAAAGG GCAGATGTTG GCTTCATGAA GCCAATAGAG AATTTCCTAA GAAACTTAAA 
CTTGAAGCTA ATGAAGAATT GTGTCCGAGG GTGTTTCTTC TACCAAGTTC TAAAACTGTA 
CCACCTGGCG CTGAACAACA TTCCTCTAAT CGAGTACCAA TTGAATTAAC TGCAGAGAAG 
AAGTCTAACT TGGAGAAGAT GTATTCTTAC AAAGCATCTG ATAAAATTTC TTCTGGGAGT 
AACATTCCTG AAAAAAGCAT CATAGTACAC TCACCAGGAA AATCTTACAA GTCTTCAGAA 
CCTGTTATTT CAGCTGAAGA GCAGGAGACT CAGACTGTGT TGTATGGCAT ATTGGTGGAA 
GCCAGGCAGA AACATGCCGG TAAAATGGAT GTTCCTCCAG CCATTCTAGC AACAAATAAG 
ATACTATTGG AAATGGCCAA AATGAGTCAG ACCTCTTTTC AAGTACAAAA CCTTGGGAAG 
AACCAAGGAA CAGTGTGGTG GAGGAAAATG AAACATGTTC CCTTTCACAG TCTGCGGCCA 
TCACGTATTC CTTATTCCAA GAAAAGAAGA TGTCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013975550.1
CDS	167..3805
Translation	MSLLKMNEKKLERTPQPRKLPEWMSMQNETCTAVETKASIQKSVFEDDLSFLEFTGSIVYSYEASDCSFLSEDISMNLSTGGVVGFDMEWPPVYTKRKPSRVALIQLCVSENKCYLFHISSMSVFPQGLKMLLENEAIKKAGVGIKGDQRKLLCDFDIDLKNFVELTDVANEKLKCTETWSLNGLVKHLFGKQLLKDRSIRCSNWGDFPLTEDQKLYAATDAYAGLIIYQKLEILGNAVERFAINKKEEEILPIDVKKQLTSISEEMMDLAKHLPDALKKLEDPQRVSLLLSAISENLYSLRKMIIGSPEAELRHSTNVHLLSLGDPTADEVQQKQIREHKFFPKVKDETWDTTFDNLIKHDEGTEPQNKVKQKEDGLEDGLEVGNKLKTNTERICLMSLDITEHELQILEQQAQEKFLNDVTCKSPEHLSSKDNEEDLSYVIESDEDLEKEMIKSLENLNTGMADPIHSKCIKMEGNLDVPFEDDEDEIEAIEEEDDNVEDYSLPRPNARQINFLKIYFGHSSFKPVQWKVIHSVLEERRDNVVVMATGYGKSLCFQYPPVYSGRIGLVISPLISLMEDQVLQLKMSNIPACFLGSAQSKNVLEDIKLGKYRIVYITPEFCSGNLFLLQQLQADIGITLIAVDEAHCISEWGHDFRSSFRTLGSLKAILPSVPIVALTATASSSIREDIVRCLNLKNPQITCTGFDRPNLYLEVRRKTGSILQDLDQFLVQKTSSIWEFEGPTIIYCPSRKTTEQVTAELRKLKLACGAYHAGLDIKSRREIHHRFMRDEIQCVVATIAFGMGINKADIRKVIHYGAPKEMESYYQEIGRAGRDGLQSSCHVLWAPADICLNRHRLSEITSEKFRLYKLKMMAKMEKYLHSTRCRRQLILSHFEDKQLRKASVGIMGTEQCCDNCRSRLDQCSSIDDSDDASQDFGPQTFQLLSAVKILGEKFGIGLPILFLQGSNSQRLEDKYRRHSLFGTGKDQKESWWKALSRQLMAEGFLVEVPGRQKFIRICTLTEKGRCWLHEANREFPKKLKLEANEELCPRVFLLPSSKTVPPGAEQHSSNRVPIELTAEKKSNLEKMYSYKASDKISSGSNIPEKSIIVHSPGKSYKSSEPVISAEEQETQTVLYGILVEARQKHAGKMDVPPAILATNKILLEMAKMSQTSFQVQNLGKNQGTVWWRKMKHVPFHSLRPSRIPYSKKRRCL

Target ORF information:

RefSeq Version	XM_014120075.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris Werner syndrome, RecQ helicase-like (WRN), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120075.1

ORF Insert Sequence:

ATGAGCCTAT TAAAGATGAA TGAAAAAAAA TTGGAAAGAA CTCCACAGCC GCGAAAACTT 
CCTGAATGGA TGTCTATGCA GAATGAGACA TGTACTGCAG TAGAAACAAA GGCATCGATT 
CAGAAGAGTG TTTTTGAAGA CGACCTCTCT TTCTTAGAAT TCACTGGATC TATTGTTTAT 
AGTTATGAAG CTAGTGATTG TTCTTTTCTA TCAGAAGATA TTAGCATGAA TCTGTCTACT 
GGAGGTGTGG TGGGTTTTGA CATGGAATGG CCACCAGTAT ACACTAAAAG GAAACCGAGC 
CGAGTTGCAC TGATTCAGTT ATGTGTGTCA GAGAACAAAT GTTACTTGTT TCATATTTCT 
TCTATGTCAG TTTTTCCTCA GGGGTTAAAA ATGTTGCTTG AAAATGAAGC AATTAAAAAA 
GCAGGTGTAG GAATCAAAGG AGATCAGCGG AAACTTCTAT GTGACTTTGA CATCGATTTG 
AAGAATTTTG TGGAGCTGAC AGATGTTGCC AATGAAAAGC TGAAATGTAC AGAGACTTGG 
AGCCTCAATG GTTTGGTTAA ACACCTCTTT GGCAAACAGC TTCTGAAAGA CAGATCTATC 
CGTTGTAGCA ATTGGGGTGA TTTTCCTCTC ACTGAGGATC AGAAACTGTA TGCAGCCACT 
GATGCGTATG CTGGTCTCAT CATTTATCAG AAATTAGAGA TTTTAGGTAA TGCTGTGGAA 
AGGTTTGCTA TAAATAAAAA AGAGGAGGAA ATCCTACCTA TTGATGTGAA GAAACAATTG 
ACTTCAATCT CTGAGGAGAT GATGGATCTG GCCAAGCATC TTCCTGACGC TTTGAAAAAA 
TTAGAAGATC CACAGAGGGT TTCTCTACTA TTAAGTGCTA TTTCAGAAAA TCTGTATTCA 
TTGAGGAAGA TGATAATTGG TTCTCCTGAG GCCGAATTGA GGCACAGCAC TAATGTTCAC 
TTATTATCAT TGGGAGATCC AACTGCTGAT GAAGTACAGC AGAAACAAAT TAGAGAACAT 
AAATTTTTTC CTAAAGTTAA AGATGAGACT TGGGACACAA CATTTGATAA TTTAATTAAA 
CATGATGAAG GAACCGAACC TCAAAACAAA GTGAAACAAA AAGAAGATGG ACTTGAAGAT 
GGATTAGAAG TTGGCAATAA ATTGAAAACG AACACAGAAA GAATTTGTTT GATGTCATTA 
GATATAACAG AACACGAACT CCAGATTTTG GAACAGCAGG CTCAGGAAAA ATTTCTTAAT 
GATGTCACTT GCAAATCTCC TGAGCATTTA TCTTCCAAAG ATAATGAAGA GGATCTATCT 
TATGTAATTG AAAGCGATGA AGATTTAGAA AAGGAAATGA TTAAGTCTTT AGAAAATTTA 
AATACTGGCA TGGCAGATCC AATTCATTCA AAATGCATAA AAATGGAAGG AAATCTGGAT 
GTTCCTTTTG AAGATGATGA AGATGAGATT GAAGCTATTG AAGAAGAAGA TGATAATGTT 
GAAGACTATT CATTACCAAG ACCTAATGCA AGGCAAATTA ATTTCCTCAA GATCTATTTT 
GGCCATTCCA GTTTTAAACC GGTTCAGTGG AAGGTGATTC ATTCTGTATT GGAAGAAAGG 
AGAGATAATG TTGTTGTGAT GGCAACTGGA TATGGAAAGA GTTTGTGCTT CCAGTACCCA 
CCTGTGTATT CAGGGAGGAT TGGTCTTGTC ATCTCTCCTC TTATTTCTTT GATGGAAGAC 
CAAGTGCTCC AGCTTAAGAT GTCCAATATT CCAGCTTGTT TTCTGGGATC AGCACAGTCA 
AAAAATGTTC TAGAAGATAT TAAATTAGGC AAATATCGGA TTGTATACAT AACTCCAGAA 
TTCTGTTCTG GTAACTTGTT CCTACTCCAG CAACTTCAGG CTGATATTGG TATCACACTT 
ATTGCTGTGG ATGAGGCTCA CTGTATTTCT GAATGGGGAC ATGATTTTAG AAGTTCATTC 
AGGACTTTGG GCTCCCTAAA GGCAATACTC CCATCGGTTC CAATTGTTGC ACTTACTGCT 
ACTGCAAGTT CTTCAATCCG GGAAGACATT GTACGCTGCT TAAACCTGAA GAATCCTCAG 
ATTACCTGTA CTGGTTTTGA TCGACCAAAC TTGTATTTAG AAGTTAGGCG AAAAACAGGA 
AGCATCCTTC AGGATCTTGA CCAATTTCTT GTCCAAAAGA CAAGTTCCAT CTGGGAATTT 
GAAGGTCCAA CTATCATCTA TTGTCCTTCC CGAAAAACAA CAGAACAAGT TACAGCTGAA 
CTTAGGAAAC TGAAGTTAGC CTGTGGAGCA TACCATGCAG GCTTGGATAT TAAATCAAGG 
AGAGAAATTC ATCATAGGTT CATGAGAGAT GAAATTCAGT GTGTTGTAGC TACCATAGCT 
TTTGGAATGG GCATTAATAA AGCTGACATT CGCAAAGTCA TTCATTATGG TGCTCCTAAG 
GAAATGGAAT CATATTACCA GGAGATTGGG AGAGCTGGCC GTGATGGACT TCAGAGTTCT 
TGTCATGTAC TCTGGGCTCC AGCAGACATT TGCTTAAATA GGCACCGCCT TAGTGAGATA 
ACCAGTGAAA AGTTTCGATT ATACAAATTA AAGATGATGG CGAAGATGGA AAAATATCTT 
CATTCCACAA GATGTAGGCG ACAACTCATC TTGTCTCATT TTGAAGACAA ACAACTACGA 
AAGGCCTCCG TGGGTATTAT GGGAACTGAA CAATGCTGTG ATAATTGCAG ATCCAGATTG 
GATCAATGCT CTTCCATTGA TGATTCAGAT GACGCATCAC AGGATTTTGG TCCACAAACA 
TTCCAGCTAT TATCTGCTGT GAAAATCTTA GGAGAAAAGT TTGGAATTGG GCTTCCGATT 
TTATTTCTCC AAGGATCTAA TTCCCAGCGT CTTGAAGATA AGTATCGCAG ACACAGTTTA 
TTTGGCACTG GCAAGGATCA AAAAGAGAGT TGGTGGAAGG CTTTGTCCCG TCAGCTCATG 
GCTGAAGGAT TCTTGGTAGA AGTTCCTGGG AGACAAAAAT TTATAAGGAT TTGCACTCTT 
ACAGAAAAGG GCAGATGTTG GCTTCATGAA GCCAATAGAG AATTTCCTAA GAAACTTAAA 
CTTGAAGCTA ATGAAGAATT GTGTCCGAGG GTGTTTCTTC TACCAAGTTC TAAAACTGTA 
CCACCTGGCG CTGAACAACA TTCCTCTAAT CGAGTACCAA TTGAATTAAC TGCAGAGAAG 
AAGTCTAACT TGGAGAAGAT GTATTCTTAC AAAGCATCTG ATAAAATTTC TTCTGGGAGT 
AACATTCCTG AAAAAAGCAT CATAGTACAC TCACCAGGAA AATCTTACAA GTCTTCAGAA 
CCTGTTATTT CAGCTGAAGA GCAGGAGACT CAGACTGTGT TGTATGGCAT ATTGGTGGAA 
GCCAGGCAGA AACATGCCGG TAAAATGGAT GTTCCTCCAG CCATTCTAGC AACAAATAAG 
ATACTATTGG AAATGGCCAA AATGAGTCAG ACCTCTTTTC AAGTACAAAA CCTTGGGAAG 
AACCAAGGAA CAGTGTGGTG GAGGAAAATG AAACATGTTC CCTTTCACAG TCTGCGGCCA 
TCACGTATTC CTTATTCCAA GAAAAGAAGA TGTCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	WRN
Entrez Gene ID	493997
Full Name	Werner syndrome RecQ like helicase
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

Homo sapiens (human)	WRN	NP_000544.2
Mus musculus (house mouse)	Wrn	NP_035851.3
Bos taurus (cattle)	WRN	NP_001137329.2
Caenorhabditis elegans (roundworm)	wrn-1	NP_495324.2
Canis lupus familiaris (dog)	WRN	XP_539984.4
Gallus gallus (chicken)	WRN	XP_001235033.2
Xenopus tropicalis (tropical clawed frog)	wrn	XP_002939304.1
Pan troglodytes (chimpanzee)	WRN	XP_528104.2
	WRN	XP_001085031.1
Rattus norvegicus (Norway rat)	RGD1564788	XP_006253317.1
Danio rerio (zebrafish)	wrn	XP_697980.5
Oryza sativa (rice)	Os07g0681600	NP_001060655.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

WRN cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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