CFTR cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	CFTR
Entrez Gene ID	492302
Full Name	cystic fibrosis transmembrane conductance regulator
General protein information	Preferred Names cystic fibrosis transmembrane conductance regulator Names cystic fibrosis transmembrane conductance regulator ATP-binding cassette sub-family C member 7 ATP-binding cassette transporter sub-family C member 7 cAMP-dependent chloride channel channel conductance-controlling ATPase
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001007143.1	NP_001007144.1	cystic fibrosis transmembrane conductance regulator

Pathways from BioSystems

Homologies

	CFTR	NP_001028110.1
Pan troglodytes (chimpanzee)	CFTR	NP_001073386.1
Rattus norvegicus (Norway rat)	Cftr	NP_113694.1
Canis lupus familiaris (dog)	CFTR	NP_001007144.1
Mus musculus (house mouse)	Cftr	NP_066388.1
Xenopus tropicalis (tropical clawed frog)	cftr	XP_002935002.2
Homo sapiens (human)	CFTR	NP_000483.3
Bos taurus (cattle)	CFTR	NP_776443.1
Gallus gallus (chicken)	CFTR	NP_001099136.1
Danio rerio (zebrafish)	cftr	NP_001038348.1

Gene Ontology
Bibliography

Related articles in PubMed

Naturally occurring mutations in the canine CFTR gene.
Spadafora D, Hawkins EC, Murphy KE, Clark LA, Ballard ST
Physiological genomics42(3)480-5(2010 Aug)

Comparative analyses of multi-species sequences from targeted genomic regions.
Thomas JW, Touchman JW, Blakesley RW, Bouffard GG, Beckstrom-Sternberg SM, Margulies EH, Blanchette M, Siepel AC, Thomas PJ, McDowell JC, Maskeri B, Hansen NF, Schwartz MS, Weber RJ, Kent WJ, Karolchik D, Bruen TC, Bevan R, Cutler DJ, Schwartz S, Elnitski L, Idol JR, Prasad AB, Lee-Lin SQ, Maduro VV, Summers TJ, Portnoy ME, Dietrich NL, Akhter N, Ayele K, Benjamin B, Cariaga K, Brinkley CP, Brooks SY, Granite S, Guan X, Gupta J, Haghighi P, Ho SL, Huang MC, Karlins E, Laric PL, Legaspi R, Lim MJ, Maduro QL, Masiello CA, Mastrian SD, McCloskey JC, Pearson R, Stantripop S, Tiongson EE, Tran JT, Tsurgeon C, Vogt JL, Walker MA, Wetherby KD, Wiggins LS, Young AC, Zhang LH, Osoegawa K, Zhu B, Zhao B, Shu CL, De Jong PJ, Lawrence CE, Smit AF, Chakravarti A, Haussler D, Green P, Miller W, Green ED
Nature424(6950)788-93(2003 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Naturally occurring CFTR mutations are relatively common in domestic dogs.
Title: Naturally occurring mutations in the canine CFTR gene.

The following CFTR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CFTR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe00158	NM_001007143.1 Latest version!	Canis lupus familiaris CF transmembrane conductance regulator (CFTR), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CFTR ( NM_001007143.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001007144.1 Canis lupus familiaris CF transmembrane conductance regulator (CFTR), mRNA.

Price & Availability↑

CloneID	OCe00158
Clone ID Related Accession (Same CDS sequence)	NM_001007143.1
Accession Version	NM_001007143.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4452bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-18
Organism	Canis lupus familiaris(dog)
Product	cystic fibrosis transmembrane conductance regulator
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY780429.1. On Jan 18, 2005 this sequence version replaced XM_532527.1. ##Evidence-Data-START## Transcript exon combination :: AY780429.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00013598, SAMN07419779 [ECO:0000348] ##Evidence-Data-END##

ATGCAGAGGT CGCCCCTGGA AAAGGCCAGC GTCCTCTCCA AACTTTTCTT CAGCTGGACC 
AGACCAATTT TGATAAAAGG CTATAGACAA CGCCTGGAAT TATCAGACAT ATACCAAGTC 
CCTTCTACTG ATTCTGCTGA TCATCTGTCT GAAAAATTGG AAAGAGAATG GGACAGAGAG 
CTGGCATCAA AGAAGAACCC CAAACTTATT AATGCCCTTC GGCGATGCTT TTTCTGGAGA 
TTTGCGTTCT ATGGAATCTT ATTATATTTA GGGGAAGTCA CCAAAGCAGT CCAGCCTCTC 
TTACTTGGAA GAATCATAGC TTCCTATGAT CCAGATAACA AGCAGGAACG CTCCATTGCA 
ATCTACCTAG CCATTGGCTT ATGCCTTCTC TTTATCATGA GGCCGCTGCT CCTGCACCCA 
GCCATTTTTG GCCTTCATCA CATTGGAATG CAGATCAGAA TAGCTATGTT TAGTTTGATT 
TATAAGAAGA CTTTAAAGCT GTCAAGCCGT GTTCTGGATA AAATAAGTAT TGGACAACTT 
GTTAGTCTCC TTTCCAACAA CCTGAACAAA TTCGATGAAG GACTTGCACT GGCGCATTTC 
GTGTGGATTG CTCCTCTACA AGTGACGCTC CTGATGGGGC TGCTCTGGGA TTTGTTACAA 
GCCTCTGCCT TCTGTGGTCT TGCTTTCCTC ATAGTCCTCG CCCTTGTCCA AGCTGGTTTG 
GGAAGAATGA TAATGAAGTA CAGAGATCAG AGAGCTGGAA AGATCAATGA AAGACTCGTG 
ATCACCTCAG AAGTGATTGA AAATATCCAA TCTGTTAAGG CATACTGCTG GGAGGAAGCA 
ATGGAAAAGA TAATTGAAAA CATAAGACAA ACAGAGCTGA AACTGACCCG GAAGGCAGCC 
CATGTGAGAT ACTTCAATAG CTCAGCCTTC TTCTTCTCAG GGTTCTTTGT GGTGTCTTTA 
TCTGTGCTTC CCTATGCACT GATCAAAACA ATCATCCTTC GCAAAATATT CACAACCATC 
TCATTCTGCA TCGTTTTGCG CATGGCAGTC ACTCGGCAAT TTCCCTGGGC TGTACAAACA 
TGGTATGACT CTCTTGGAGC AATAAACAAA ATACAGGATT TCTTACAAAA GCAAGAGTAT 
AAGACGTTGG AATATAACTT AACAACTACG GAAGTAATGA TGGAGAATGT AACAGCCTTC 
TGGGAGGAGG GATTTGGGGA ATTACTTGAG AAAGCAAAAG AAAACAGTAA TGACAGAAAA 
ATTTCCAATG GGGACAACAG CCTCTTCTTC AGCAACTTCT CACTTCTTGG TACTCCTGTC 
CTAAAAGACA TCAATTTCAA GATAGAAAGA GGACAGTTGT TGGCGGTTGC TGGATCTACT 
GGAGCAGGCA AGACTTCACT CCTAATGATG ATTATGGGAG AACTGGAGCC TTCAGAGGGT 
AAAATTAAGC ACAGTGGAAG AATTTCATTC TGCTCTCAGT TTTCTTGGAT TATGCCTGGC 
ACCATTAAAG AAAACATCAT CTTTGGTGTT TCCTATGATG AGTATAGATA TAGGAGTGTC 
ATCAAAGCAT GCCAACTAGA GGAGGACATT TCCAAGTTTG CAGAGAAGGA CAACATCGTT 
CTTGGAGAAG GTGGCGTCAC GCTGAGCGGA GGGCAAAGAG CAAGAATTTC CCTAGCAAGA 
GCAGTATATA AAGATGCTGA TTTATATCTC TTAGACTCTC CTTTTGGATA CCTGGATGTT 
TTAACAGAAA AAGAAATATT TGAAAGCTGT GTCTGTAAAT TGATGGCTAA CAAAACTAGG 
ATCTTGGTCA CTTCTAAAAT GGAACATTTA AAGAAAGCTG ACAAAGTCTT AATTTTACAT 
GAAGGTAGCT GCTATTTTTA TGGGACATTT TCTGAATTAC AAAGTCTCCG GCCCGACTTC 
AGCTCGAAGC TCATGGGCTA TGATTCTTTT GACCAGTTCA GTCCAGAAAG AAGAAACTCC 
ATCATCACTG AGACTTTACG GCGTTTCTCC TTAGAAGGAG ATGCGGCCGT GCCCTGGAAT 
GAAACAAAAA AGCAATCTTT TAAACAGACT GGAGAGTTTG GTGAAAAAAG GAAGAACTCT 
ATTCTTAATC CAATCAACTC CATAAGAAAA TTTTCAATTG TACAAAAGAC CCCGTTACCC 
ATGAATGGCA TCGAAGGAGA GTCTGAGGTG CCAGTAGAGC GAAGGCTGTC CTTATTTCCC 
GACTGTGAGC AAGGAGAGGC CATCCTGCCT CGGAGCAACA TGATCAACAC TGGCCCCACG 
CTCCGGAGAC AACGAAGACA GTCTGTTCTG AACCTGATGA CGCGCTCCTC CGTGAACCAA 
GGTCAGAGCA TTCACCGAAG GACAACAGCA TCCACGCGAA AAATGTCACT GGCCCCTCAG 
GCAAATCTAA CTGAAATGGA TATATATTCC AGACGGTTAT CTCAAGACAG TGGCTTGGAA 
ATAAGTGAAG AAATTAATGA AGAGGATTTA AAGGAGTGCT TTTTTGATGA TGTCGAGAGC 
ATACCACCAG TGACTACATG GAACACATAC CTTCGATATA TTACTGTCCA CAAGAACTTA 
ATTTTTGTGC TAATTTGGTG CTTAGTTATT TTTCTGGCTG AGGTGGCTGT TTCATTGGTT 
GTATTGTGGA TACTTAGAAA CTTGTCTTCT CAAGACAAAG GGAATAGCAC TCAGAGCGTA 
AACAGCAGTT ATGCAGTGAT CTTCACCAGT ACCAGCGCAT ATTATATTTT CTACATTTAT 
GTGGGAGTAG CTGATACTTT GCTTGCTCTG GGACTCTTCA GAGGTTTACC ACTGGTGCAT 
ACTCTAATCA CAGCGTCGAA AATTTTACAC CACAAAATGT TACATTCTGT TCTTCAGGCA 
CCTATGTCAA CCCTCAACAC ACTGAAAGCA GGTGGGATTC TTAATAGATT CCCCAAAGAT 
ATAGCGATTT TGGATGATCT TCTGCCCCTT ACCATATTTG ACTTCATCCA GTTGTTATTA 
ATTGTGATCG GAGCTGTGGC GGTGGTGTCA GTTTTACAGC CCTACATCTT CCTAGCGACA 
GTGCCAGTGA TAGCGGCCTT TATTATCCTG CGGGCCTACT TCCTCCACAC CTCACAGCAA 
CTCAAGCAAC TGGAATCTGA AGGCAGGAGT CCAATTTTCA CTCATCTTCT TACAAGTTTA 
AAAGGACTGT GGACACTTCG GGCCTTTGGA CGCCAGCCTT ACTTTGAGAC TTTGTTTCAC 
AAAGCTCTGA ACTTACATAC TGCCAACTGG TTCTTGTACC TGTCAACGCT GCGCTGGTTC 
CAAATGAGAA TGGAAATTAT TTTTGTCATA TTCTTCATTG CTGTTACCTT CATCTCCATT 
TTAACAACAG GTGAAGGAGA AGGAACAGTT GGTATTATTC TAACATTAGC CATGAATATC 
ATGGGTACGT TGCAGTGGGC TGTAAACCCC AGCATAGAGG TGGACAGCTT GATGCGATCG 
GTGAGCCGAG TCTTCAAGTT TATTGACATG CCTACAGAAG AAAGTAAACC ACCTACCAAG 
TCATTCAAAC CATCCAAGGA TGCCCAACTC TCAAAAGTTA CGATTACTGA GAATCGGCAT 
GTGAGGGAAG ATGATATCTG GCCCTCAGGG GGCCAAATGA CTGTCAAAGA CCTCACAGCA 
AAGTATATAG ATGGTGGGAA TGCCATATTA GAGAACATTT CCTTCTCAAT AAGTCCTGGC 
CAGAGGGTGG GCCTCTTGGG AAGAACTGGA TCAGGGAAAA GTACTTTGTT ATCTGCTCTT 
TTGAGACTGG TGAACACTGA GGGAGAAATC CAAATAGATG GCGTGTCTTG GGACTCAATC 
CCTTTGCAAG AATGGAGGAG AGCCTTTGGG GTGATCCCAC AGAAAGTATT TATATTCTCT 
GGAACATTTA GAAAAAACTT GGATCCCTAT GGACAGTGGA ATGATCAAGA AATATGGAAA 
GTTGCGGATG AGGTTGGACT CAGATCTGTG ATAGAGCAGT TTCCTGGGAA GCTCGATTTC 
GTCCTGGTGG ATGGGGGTTA CGTCCTAAGC CATGGCCACA AGCAGTTGAT GTGCTTGGCC 
AGATCTGTCC TCAGGAAGGC GAAAATCCTG CTGCTCGATG AACCCAGTGC TCATTTGGAT 
CCAATAACAT ACCAAATCAT TCGAAGAACC CTAAAGCAAG CGTTTGCAGA TTGCACAGTG 
ATCCTTTCTG AACACAGGAT AGAAGCGATG TTGGAATGTC AACGATTCTT GGTCATCGAG 
GACAGCAGGC TGCGGCAGTT CGAGTCCATC CAGAGGCTGC TGAGCGAGAG GAGCGCCTTC 
CGGCAGGCCA TCGGGCCCCC CGAGCGGCCC GGCCTCCTCC CGCACCGGCT CTCCAGCCGG 
CAGAGGTCCC CTTCCAGGAT CGCGGCCCTG AAGGAGGAGA CGGAAGACGA GGTGCAGGAC 
ACAAGGCTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001007144.1
CDS	1..4452
Translation	MQRSPLEKASVLSKLFFSWTRPILIKGYRQRLELSDIYQVPSTDSADHLSEKLEREWDRELASKKNPKLINALRRCFFWRFAFYGILLYLGEVTKAVQPLLLGRIIASYDPDNKQERSIAIYLAIGLCLLFIMRPLLLHPAIFGLHHIGMQIRIAMFSLIYKKTLKLSSRVLDKISIGQLVSLLSNNLNKFDEGLALAHFVWIAPLQVTLLMGLLWDLLQASAFCGLAFLIVLALVQAGLGRMIMKYRDQRAGKINERLVITSEVIENIQSVKAYCWEEAMEKIIENIRQTELKLTRKAAHVRYFNSSAFFFSGFFVVSLSVLPYALIKTIILRKIFTTISFCIVLRMAVTRQFPWAVQTWYDSLGAINKIQDFLQKQEYKTLEYNLTTTEVMMENVTAFWEEGFGELLEKAKENSNDRKISNGDNSLFFSNFSLLGTPVLKDINFKIERGQLLAVAGSTGAGKTSLLMMIMGELEPSEGKIKHSGRISFCSQFSWIMPGTIKENIIFGVSYDEYRYRSVIKACQLEEDISKFAEKDNIVLGEGGVTLSGGQRARISLARAVYKDADLYLLDSPFGYLDVLTEKEIFESCVCKLMANKTRILVTSKMEHLKKADKVLILHEGSCYFYGTFSELQSLRPDFSSKLMGYDSFDQFSPERRNSIITETLRRFSLEGDAAVPWNETKKQSFKQTGEFGEKRKNSILNPINSIRKFSIVQKTPLPMNGIEGESEVPVERRLSLFPDCEQGEAILPRSNMINTGPTLRRQRRQSVLNLMTRSSVNQGQSIHRRTTASTRKMSLAPQANLTEMDIYSRRLSQDSGLEISEEINEEDLKECFFDDVESIPPVTTWNTYLRYITVHKNLIFVLIWCLVIFLAEVAVSLVVLWILRNLSSQDKGNSTQSVNSSYAVIFTSTSAYYIFYIYVGVADTLLALGLFRGLPLVHTLITASKILHHKMLHSVLQAPMSTLNTLKAGGILNRFPKDIAILDDLLPLTIFDFIQLLLIVIGAVAVVSVLQPYIFLATVPVIAAFIILRAYFLHTSQQLKQLESEGRSPIFTHLLTSLKGLWTLRAFGRQPYFETLFHKALNLHTANWFLYLSTLRWFQMRMEIIFVIFFIAVTFISILTTGEGEGTVGIILTLAMNIMGTLQWAVNPSIEVDSLMRSVSRVFKFIDMPTEESKPPTKSFKPSKDAQLSKVTITENRHVREDDIWPSGGQMTVKDLTAKYIDGGNAILENISFSISPGQRVGLLGRTGSGKSTLLSALLRLVNTEGEIQIDGVSWDSIPLQEWRRAFGVIPQKVFIFSGTFRKNLDPYGQWNDQEIWKVADEVGLRSVIEQFPGKLDFVLVDGGYVLSHGHKQLMCLARSVLRKAKILLLDEPSAHLDPITYQIIRRTLKQAFADCTVILSEHRIEAMLECQRFLVIEDSRLRQFESIQRLLSERSAFRQAIGPPERPGLLPHRLSSRQRSPSRIAALKEETEDEVQDTRL

Target ORF information:

RefSeq Version	NM_001007143.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris CF transmembrane conductance regulator (CFTR), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001007143.1

ORF Insert Sequence:

ATGCAGAGGT CGCCCCTGGA AAAGGCCAGC GTCCTCTCCA AACTTTTCTT CAGCTGGACC 
AGACCAATTT TGATAAAAGG CTATAGACAA CGCCTGGAAT TATCAGACAT ATACCAAGTC 
CCTTCTACTG ATTCTGCTGA TCATCTGTCT GAAAAATTGG AAAGAGAATG GGACAGAGAG 
CTGGCATCAA AGAAGAACCC CAAACTTATT AATGCCCTTC GGCGATGCTT TTTCTGGAGA 
TTTGCGTTCT ATGGAATCTT ATTATATTTA GGGGAAGTCA CCAAAGCAGT CCAGCCTCTC 
TTACTTGGAA GAATCATAGC TTCCTATGAT CCAGATAACA AGCAGGAACG CTCCATTGCA 
ATCTACCTAG CCATTGGCTT ATGCCTTCTC TTTATCATGA GGCCGCTGCT CCTGCACCCA 
GCCATTTTTG GCCTTCATCA CATTGGAATG CAGATCAGAA TAGCTATGTT TAGTTTGATT 
TATAAGAAGA CTTTAAAGCT GTCAAGCCGT GTTCTGGATA AAATAAGTAT TGGACAACTT 
GTTAGTCTCC TTTCCAACAA CCTGAACAAA TTCGATGAAG GACTTGCACT GGCGCATTTC 
GTGTGGATTG CTCCTCTACA AGTGACGCTC CTGATGGGGC TGCTCTGGGA TTTGTTACAA 
GCCTCTGCCT TCTGTGGTCT TGCTTTCCTC ATAGTCCTCG CCCTTGTCCA AGCTGGTTTG 
GGAAGAATGA TAATGAAGTA CAGAGATCAG AGAGCTGGAA AGATCAATGA AAGACTCGTG 
ATCACCTCAG AAGTGATTGA AAATATCCAA TCTGTTAAGG CATACTGCTG GGAGGAAGCA 
ATGGAAAAGA TAATTGAAAA CATAAGACAA ACAGAGCTGA AACTGACCCG GAAGGCAGCC 
CATGTGAGAT ACTTCAATAG CTCAGCCTTC TTCTTCTCAG GGTTCTTTGT GGTGTCTTTA 
TCTGTGCTTC CCTATGCACT GATCAAAACA ATCATCCTTC GCAAAATATT CACAACCATC 
TCATTCTGCA TCGTTTTGCG CATGGCAGTC ACTCGGCAAT TTCCCTGGGC TGTACAAACA 
TGGTATGACT CTCTTGGAGC AATAAACAAA ATACAGGATT TCTTACAAAA GCAAGAGTAT 
AAGACGTTGG AATATAACTT AACAACTACG GAAGTAATGA TGGAGAATGT AACAGCCTTC 
TGGGAGGAGG GATTTGGGGA ATTACTTGAG AAAGCAAAAG AAAACAGTAA TGACAGAAAA 
ATTTCCAATG GGGACAACAG CCTCTTCTTC AGCAACTTCT CACTTCTTGG TACTCCTGTC 
CTAAAAGACA TCAATTTCAA GATAGAAAGA GGACAGTTGT TGGCGGTTGC TGGATCTACT 
GGAGCAGGCA AGACTTCACT CCTAATGATG ATTATGGGAG AACTGGAGCC TTCAGAGGGT 
AAAATTAAGC ACAGTGGAAG AATTTCATTC TGCTCTCAGT TTTCTTGGAT TATGCCTGGC 
ACCATTAAAG AAAACATCAT CTTTGGTGTT TCCTATGATG AGTATAGATA TAGGAGTGTC 
ATCAAAGCAT GCCAACTAGA GGAGGACATT TCCAAGTTTG CAGAGAAGGA CAACATCGTT 
CTTGGAGAAG GTGGCGTCAC GCTGAGCGGA GGGCAAAGAG CAAGAATTTC CCTAGCAAGA 
GCAGTATATA AAGATGCTGA TTTATATCTC TTAGACTCTC CTTTTGGATA CCTGGATGTT 
TTAACAGAAA AAGAAATATT TGAAAGCTGT GTCTGTAAAT TGATGGCTAA CAAAACTAGG 
ATCTTGGTCA CTTCTAAAAT GGAACATTTA AAGAAAGCTG ACAAAGTCTT AATTTTACAT 
GAAGGTAGCT GCTATTTTTA TGGGACATTT TCTGAATTAC AAAGTCTCCG GCCCGACTTC 
AGCTCGAAGC TCATGGGCTA TGATTCTTTT GACCAGTTCA GTCCAGAAAG AAGAAACTCC 
ATCATCACTG AGACTTTACG GCGTTTCTCC TTAGAAGGAG ATGCGGCCGT GCCCTGGAAT 
GAAACAAAAA AGCAATCTTT TAAACAGACT GGAGAGTTTG GTGAAAAAAG GAAGAACTCT 
ATTCTTAATC CAATCAACTC CATAAGAAAA TTTTCAATTG TACAAAAGAC CCCGTTACCC 
ATGAATGGCA TCGAAGGAGA GTCTGAGGTG CCAGTAGAGC GAAGGCTGTC CTTATTTCCC 
GACTGTGAGC AAGGAGAGGC CATCCTGCCT CGGAGCAACA TGATCAACAC TGGCCCCACG 
CTCCGGAGAC AACGAAGACA GTCTGTTCTG AACCTGATGA CGCGCTCCTC CGTGAACCAA 
GGTCAGAGCA TTCACCGAAG GACAACAGCA TCCACGCGAA AAATGTCACT GGCCCCTCAG 
GCAAATCTAA CTGAAATGGA TATATATTCC AGACGGTTAT CTCAAGACAG TGGCTTGGAA 
ATAAGTGAAG AAATTAATGA AGAGGATTTA AAGGAGTGCT TTTTTGATGA TGTCGAGAGC 
ATACCACCAG TGACTACATG GAACACATAC CTTCGATATA TTACTGTCCA CAAGAACTTA 
ATTTTTGTGC TAATTTGGTG CTTAGTTATT TTTCTGGCTG AGGTGGCTGT TTCATTGGTT 
GTATTGTGGA TACTTAGAAA CTTGTCTTCT CAAGACAAAG GGAATAGCAC TCAGAGCGTA 
AACAGCAGTT ATGCAGTGAT CTTCACCAGT ACCAGCGCAT ATTATATTTT CTACATTTAT 
GTGGGAGTAG CTGATACTTT GCTTGCTCTG GGACTCTTCA GAGGTTTACC ACTGGTGCAT 
ACTCTAATCA CAGCGTCGAA AATTTTACAC CACAAAATGT TACATTCTGT TCTTCAGGCA 
CCTATGTCAA CCCTCAACAC ACTGAAAGCA GGTGGGATTC TTAATAGATT CCCCAAAGAT 
ATAGCGATTT TGGATGATCT TCTGCCCCTT ACCATATTTG ACTTCATCCA GTTGTTATTA 
ATTGTGATCG GAGCTGTGGC GGTGGTGTCA GTTTTACAGC CCTACATCTT CCTAGCGACA 
GTGCCAGTGA TAGCGGCCTT TATTATCCTG CGGGCCTACT TCCTCCACAC CTCACAGCAA 
CTCAAGCAAC TGGAATCTGA AGGCAGGAGT CCAATTTTCA CTCATCTTCT TACAAGTTTA 
AAAGGACTGT GGACACTTCG GGCCTTTGGA CGCCAGCCTT ACTTTGAGAC TTTGTTTCAC 
AAAGCTCTGA ACTTACATAC TGCCAACTGG TTCTTGTACC TGTCAACGCT GCGCTGGTTC 
CAAATGAGAA TGGAAATTAT TTTTGTCATA TTCTTCATTG CTGTTACCTT CATCTCCATT 
TTAACAACAG GTGAAGGAGA AGGAACAGTT GGTATTATTC TAACATTAGC CATGAATATC 
ATGGGTACGT TGCAGTGGGC TGTAAACCCC AGCATAGAGG TGGACAGCTT GATGCGATCG 
GTGAGCCGAG TCTTCAAGTT TATTGACATG CCTACAGAAG AAAGTAAACC ACCTACCAAG 
TCATTCAAAC CATCCAAGGA TGCCCAACTC TCAAAAGTTA CGATTACTGA GAATCGGCAT 
GTGAGGGAAG ATGATATCTG GCCCTCAGGG GGCCAAATGA CTGTCAAAGA CCTCACAGCA 
AAGTATATAG ATGGTGGGAA TGCCATATTA GAGAACATTT CCTTCTCAAT AAGTCCTGGC 
CAGAGGGTGG GCCTCTTGGG AAGAACTGGA TCAGGGAAAA GTACTTTGTT ATCTGCTCTT 
TTGAGACTGG TGAACACTGA GGGAGAAATC CAAATAGATG GCGTGTCTTG GGACTCAATC 
CCTTTGCAAG AATGGAGGAG AGCCTTTGGG GTGATCCCAC AGAAAGTATT TATATTCTCT 
GGAACATTTA GAAAAAACTT GGATCCCTAT GGACAGTGGA ATGATCAAGA AATATGGAAA 
GTTGCGGATG AGGTTGGACT CAGATCTGTG ATAGAGCAGT TTCCTGGGAA GCTCGATTTC 
GTCCTGGTGG ATGGGGGTTA CGTCCTAAGC CATGGCCACA AGCAGTTGAT GTGCTTGGCC 
AGATCTGTCC TCAGGAAGGC GAAAATCCTG CTGCTCGATG AACCCAGTGC TCATTTGGAT 
CCAATAACAT ACCAAATCAT TCGAAGAACC CTAAAGCAAG CGTTTGCAGA TTGCACAGTG 
ATCCTTTCTG AACACAGGAT AGAAGCGATG TTGGAATGTC AACGATTCTT GGTCATCGAG 
GACAGCAGGC TGCGGCAGTT CGAGTCCATC CAGAGGCTGC TGAGCGAGAG GAGCGCCTTC 
CGGCAGGCCA TCGGGCCCCC CGAGCGGCCC GGCCTCCTCC CGCACCGGCT CTCCAGCCGG 
CAGAGGTCCC CTTCCAGGAT CGCGGCCCTG AAGGAGGAGA CGGAAGACGA GGTGCAGGAC 
ACAAGGCTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Naturally occurring mutations in the canine CFTR gene.
Physiological genomics42(3)480-5(2010 Aug)
Spadafora D,Hawkins EC,Murphy KE,Clark LA,Ballard ST

Comparative analyses of multi-species sequences from targeted genomic regions.
Nature424(6950)788-93(2003 Aug)
Thomas JW,Touchman JW,Blakesley RW,Bouffard GG,Beckstrom-Sternberg SM,Margulies EH,Blanchette M,Siepel AC,Thomas PJ,McDowell JC,Maskeri B,Hansen NF,Schwartz MS,Weber RJ,Kent WJ,Karolchik D,Bruen TC,Bevan R,Cutler DJ,Schwartz S,Elnitski L,Idol JR,Prasad AB,Lee-Lin SQ,Maduro VV,Summers TJ,Portnoy ME,Dietrich NL,Akhter N,Ayele K,Benjamin B,Cariaga K,Brinkley CP,Brooks SY,Granite S,Guan X,Gupta J,Haghighi P,Ho SL,Huang MC,Karlins E,Laric PL,Legaspi R,Lim MJ,Maduro QL,Masiello CA,Mastrian SD,McCloskey JC,Pearson R,Stantripop S,Tiongson EE,Tran JT,Tsurgeon C,Vogt JL,Walker MA,Wetherby KD,Wiggins LS,Young AC,Zhang LH,Osoegawa K,Zhu B,Zhao B,Shu CL,De Jong PJ,Lawrence CE,Smit AF,Chakravarti A,Haussler D,Green P,Miller W,Green ED

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CFTR cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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