ASIP cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ASIP
Entrez Gene ID	492296
Full Name	agouti signaling protein
Synonyms	agouti
General protein information	Preferred Names agouti signaling protein Names agouti-signaling protein ASP agouti signal peptide agouti signaling protein, nonagouti homolog agouti switch protein
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014106843.1	XP_013962318.1	agouti-signaling protein isoform X1
XM_022408819.1	XP_022264527.1	agouti-signaling protein isoform X1
XM_014106845.1	XP_013962320.1	agouti-signaling protein isoform X1
NM_001007263.1	NP_001007264.1	agouti-signaling protein precursor
XM_014106843.2	XP_013962318.1	agouti-signaling protein isoform X1
XM_022408820.1	XP_022264528.1	agouti-signaling protein isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ASIP	NP_996674.1
Mus musculus (house mouse)	a	NP_056585.2
Pan troglodytes (chimpanzee)	ASIP	NP_001129099.1
	LOC709156	XP_001097667.1
Homo sapiens (human)	ASIP	NP_001663.2
Canis lupus familiaris (dog)	ASIP	NP_001007264.1
Rattus norvegicus (Norway rat)	Asip	NP_443211.1

Gene Ontology
Bibliography

Related articles in PubMed

Characterization of different 5'-untranslated exons of the ASIP gene in black-and-tan Doberman Pinscher and brindle Boxer dogs.
Ciampolini R, Cecchi F, Spaterna A, Bramante A, Bardet SM, Oulmouden A
Animal genetics44(1)114-7(2013 Feb)

A SINE insertion causes the black-and-tan and saddle tan phenotypes in domestic dogs.
Dreger DL, Schmutz SM
The Journal of heredity102 Suppl 1S11-8(2011 Sep-Oct)

Mutations in the melanocortin 1 receptor, ?-defensin103 and agouti signaling protein genes, and their association with coat color phenotypes in Akita-inu dogs.
Oguro-Okano M, Honda M, Yamazaki K, Okano K
The Journal of veterinary medical science73(7)853-8(2011 Jul)

Linkage and segregation analysis of black and brindle coat color in domestic dogs.
Kerns JA, Cargill EJ, Clark LA, Candille SI, Berryere TG, Olivier M, Lust G, Todhunter RJ, Schmutz SM, Murphy KE, Barsh GS
Genetics176(3)1679-89(2007 Jul)

Agouti sequence polymorphisms in coyotes, wolves and dogs suggest hybridization.
Schmutz SM, Berryere TG, Barta JL, Reddick KD, Schmutz JK
The Journal of heredity98(4)351-5(2007 Jul-Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results of this study suggest that differential expression of ASIP determine pigment-type switching in a MC1R and K allele-dependent manner in dogs.
Title: Characterization of different 5'-untranslated exons of the ASIP gene in black-and-tan Doberman Pinscher and brindle Boxer dogs.

A SINE insertion in intron 1 of ASIP causes the black-and-tan and saddle tan phenotypes in domestic dogs.
Title: A SINE insertion causes the black-and-tan and saddle tan phenotypes in domestic dogs.

2 key mutations in the MC1R gene and the CBD103 gene are associated with the phenotypic discriminations among brindle, red/sesame, and white coats.
Title: Mutations in the melanocortin 1 receptor, ?-defensin103 and agouti signaling protein genes, and their association with coat color phenotypes in Akita-inu dogs.

Mc1r is epistatic to variation at Agouti or K and the epistatic relationship between Agouti and K depends on the alleles being tested.
Title: Linkage and segregation analysis of black and brindle coat color in domestic dogs.

Coyote-dog hybrids were heterozygous for 2 mutations causing fawn coat color in dogs.
Title: Agouti sequence polymorphisms in coyotes, wolves and dogs suggest hybridization.

The following ASIP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ASIP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe33806	NM_001007263.1 Latest version!	Canis lupus familiaris agouti signaling protein (ASIP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OCe42634	XM_014106845.1 Latest version!	Canis lupus familiaris agouti signaling protein (ASIP), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
OCe42634	XM_022408820.1 Latest version!	Canis lupus familiaris agouti signaling protein (ASIP), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
OCe42634	XM_014106843.2 Latest version!	Canis lupus familiaris agouti signaling protein (ASIP), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
OCe42634	XM_022408819.1 Latest version!	Canis lupus familiaris agouti signaling protein (ASIP), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
View Old Accession Versions >>
OCe42634	XM_014106843.1	Canis lupus familiaris agouti signaling protein (ASIP), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ASIP ( NM_001007263.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001007264.1 Canis lupus familiaris agouti signaling protein (ASIP), mRNA.

Price & Availability↑

CloneID	OCe33806
Clone ID Related Accession (Same CDS sequence)	NM_001007263.1
Accession Version	NM_001007263.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Canis lupus familiaris(dog)
Product	agouti-signaling protein precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY714374.1. On Sep 1, 2005 this sequence version replaced XM_850514.1. ##Evidence-Data-START## Transcript exon combination :: AY714374.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4547738, SAMEA4547739 [ECO:0000348] ##Evidence-Data-END##

ATGAATATCT TCCGCCTACT CCTGGCCACC CTGCTGGTCT CCCTGTGCTT CCTCACTGCC 
TACAGCCACC TGGCTGAGGA AAAGCCCAAG GATGACAGGA GCCTAAGGAG CAACTCCTCT 
GTGAACCTTT TGGATTTCCC TTCTGTCTCT ATTGTAGCAC TGAACAAGAA ATCCAAAAAG 
ATCAGCAGAA AAGAGGCGGA AAAGAAGAGA TCTTCTAAGA AAAAGGCTTC GATGAAGAAC 
GTGGCTCGTC CCCGGCCCCC GCCACCCACC CCCTGCGTGG CCACTCGCAA CAGCTGCAAG 
TCCCCGGCGC CCGCCTGCTG TGACCCCTGC GCCTCCTGCC AGTGCCGCTT CTTCCGCAGC 
GCCTGCACCT GCCGCGTTCT CAGTCCCAGA TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001007264.1
CDS	142..537
Translation	MNIFRLLLATLLVSLCFLTAYSHLAEEKPKDDRSLRSNSSVNLLDFPSVSIVALNKKSKKISRKEAEKKRSSKKKASMKNVARPRPPPPTPCVATRNSCKSPAPACCDPCASCQCRFFRSACTCRVLSPRC

Target ORF information:

RefSeq Version	NM_001007263.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris agouti signaling protein (ASIP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001007263.1

ORF Insert Sequence:

ATGAATATCT TCCGCCTACT CCTGGCCACC CTGCTGGTCT CCCTGTGCTT CCTCACTGCC 
TACAGCCACC TGGCTGAGGA AAAGCCCAAG GATGACAGGA GCCTAAGGAG CAACTCCTCT 
GTGAACCTTT TGGATTTCCC TTCTGTCTCT ATTGTAGCAC TGAACAAGAA ATCCAAAAAG 
ATCAGCAGAA AAGAGGCGGA AAAGAAGAGA TCTTCTAAGA AAAAGGCTTC GATGAAGAAC 
GTGGCTCGTC CCCGGCCCCC GCCACCCACC CCCTGCGTGG CCACTCGCAA CAGCTGCAAG 
TCCCCGGCGC CCGCCTGCTG TGACCCCTGC GCCTCCTGCC AGTGCCGCTT CTTCCGCAGC 
GCCTGCACCT GCCGCGTTCT CAGTCCCAGA TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASIP ( XM_014106843.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013962318.1 Canis lupus familiaris agouti signaling protein (ASIP), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe42634
Clone ID Related Accession (Same CDS sequence)	XM_014106843.1 , XM_022408819.1 , XM_014106845.1 , XM_014106843.2 , XM_022408820.1
Accession Version	XM_014106843.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	agouti-signaling protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726101.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATATCT TCCGCCTACT CCTGGCCACC CTGCTGGTCT CCCTGTGCTT CCTCACTGCC 
TACAGCCACC TGGCTGAGGA AAAGCCCAAG GATGACAGGA GCCTAAGGAG CAACTCCTCT 
GTGAACCTTT TGGATTTCCC TTCTGTCTCT ATTGTAGCAC TGAACAAGAA ATCCAAAAAG 
ATCAGCAGAA AAGAGGCGGA AAAGAAGAGA TCTTCTAAGA AAAAGGCTTC GATGAAGAAC 
GTGTCTCATC CCCGGCCCCC GCCACCCACC CCCTGCGTGG CCACTCGCAA CAGCTGCAAG 
TCCCCGGCGC CCGCCTGCTG TGACCCCTGC GCCTCCTGCC AGTGCCGCTT CTTCCGCAGC 
GCCTGCACCT GCCGCGTTCT CAGTCCCAGA TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962318.1
CDS	86..481
Translation	MNIFRLLLATLLVSLCFLTAYSHLAEEKPKDDRSLRSNSSVNLLDFPSVSIVALNKKSKKISRKEAEKKRSSKKKASMKNVSHPRPPPPTPCVATRNSCKSPAPACCDPCASCQCRFFRSACTCRVLSPRC

Target ORF information:

RefSeq Version	XM_014106843.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris agouti signaling protein (ASIP), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014106843.1

ORF Insert Sequence:

ATGAATATCT TCCGCCTACT CCTGGCCACC CTGCTGGTCT CCCTGTGCTT CCTCACTGCC 
TACAGCCACC TGGCTGAGGA AAAGCCCAAG GATGACAGGA GCCTAAGGAG CAACTCCTCT 
GTGAACCTTT TGGATTTCCC TTCTGTCTCT ATTGTAGCAC TGAACAAGAA ATCCAAAAAG 
ATCAGCAGAA AAGAGGCGGA AAAGAAGAGA TCTTCTAAGA AAAAGGCTTC GATGAAGAAC 
GTGTCTCATC CCCGGCCCCC GCCACCCACC CCCTGCGTGG CCACTCGCAA CAGCTGCAAG 
TCCCCGGCGC CCGCCTGCTG TGACCCCTGC GCCTCCTGCC AGTGCCGCTT CTTCCGCAGC 
GCCTGCACCT GCCGCGTTCT CAGTCCCAGA TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASIP ( XM_022408819.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022264527.1 Canis lupus familiaris agouti signaling protein (ASIP), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe42634
Clone ID Related Accession (Same CDS sequence)	XM_014106843.1 , XM_022408819.1 , XM_014106845.1 , XM_014106843.2 , XM_022408820.1
Accession Version	XM_022408819.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	agouti-signaling protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATATCT TCCGCCTACT CCTGGCCACC CTGCTGGTCT CCCTGTGCTT CCTCACTGCC 
TACAGCCACC TGGCTGAGGA AAAGCCCAAG GATGACAGGA GCCTAAGGAG CAACTCCTCT 
GTGAACCTTT TGGATTTCCC TTCTGTCTCT ATTGTAGCAC TGAACAAGAA ATCCAAAAAG 
ATCAGCAGAA AAGAGGCGGA AAAGAAGAGA TCTTCTAAGA AAAAGGCTTC GATGAAGAAC 
GTGTCTCATC CCCGGCCCCC GCCACCCACC CCCTGCGTGG CCACTCGCAA CAGCTGCAAG 
TCCCCGGCGC CCGCCTGCTG TGACCCCTGC GCCTCCTGCC AGTGCCGCTT CTTCCGCAGC 
GCCTGCACCT GCCGCGTTCT CAGTCCCAGA TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022264527.1
CDS	123..518
Translation	MNIFRLLLATLLVSLCFLTAYSHLAEEKPKDDRSLRSNSSVNLLDFPSVSIVALNKKSKKISRKEAEKKRSSKKKASMKNVSHPRPPPPTPCVATRNSCKSPAPACCDPCASCQCRFFRSACTCRVLSPRC

Target ORF information:

RefSeq Version	XM_022408819.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris agouti signaling protein (ASIP), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022408819.1

ORF Insert Sequence:

ATGAATATCT TCCGCCTACT CCTGGCCACC CTGCTGGTCT CCCTGTGCTT CCTCACTGCC 
TACAGCCACC TGGCTGAGGA AAAGCCCAAG GATGACAGGA GCCTAAGGAG CAACTCCTCT 
GTGAACCTTT TGGATTTCCC TTCTGTCTCT ATTGTAGCAC TGAACAAGAA ATCCAAAAAG 
ATCAGCAGAA AAGAGGCGGA AAAGAAGAGA TCTTCTAAGA AAAAGGCTTC GATGAAGAAC 
GTGTCTCATC CCCGGCCCCC GCCACCCACC CCCTGCGTGG CCACTCGCAA CAGCTGCAAG 
TCCCCGGCGC CCGCCTGCTG TGACCCCTGC GCCTCCTGCC AGTGCCGCTT CTTCCGCAGC 
GCCTGCACCT GCCGCGTTCT CAGTCCCAGA TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASIP ( XM_014106845.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013962320.1 Canis lupus familiaris agouti signaling protein (ASIP), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe42634
Clone ID Related Accession (Same CDS sequence)	XM_014106843.1 , XM_022408819.1 , XM_014106845.1 , XM_014106843.2 , XM_022408820.1
Accession Version	XM_014106845.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	agouti-signaling protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726101.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATATCT TCCGCCTACT CCTGGCCACC CTGCTGGTCT CCCTGTGCTT CCTCACTGCC 
TACAGCCACC TGGCTGAGGA AAAGCCCAAG GATGACAGGA GCCTAAGGAG CAACTCCTCT 
GTGAACCTTT TGGATTTCCC TTCTGTCTCT ATTGTAGCAC TGAACAAGAA ATCCAAAAAG 
ATCAGCAGAA AAGAGGCGGA AAAGAAGAGA TCTTCTAAGA AAAAGGCTTC GATGAAGAAC 
GTGTCTCATC CCCGGCCCCC GCCACCCACC CCCTGCGTGG CCACTCGCAA CAGCTGCAAG 
TCCCCGGCGC CCGCCTGCTG TGACCCCTGC GCCTCCTGCC AGTGCCGCTT CTTCCGCAGC 
GCCTGCACCT GCCGCGTTCT CAGTCCCAGA TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962320.1
CDS	70..465
Translation	MNIFRLLLATLLVSLCFLTAYSHLAEEKPKDDRSLRSNSSVNLLDFPSVSIVALNKKSKKISRKEAEKKRSSKKKASMKNVSHPRPPPPTPCVATRNSCKSPAPACCDPCASCQCRFFRSACTCRVLSPRC

Target ORF information:

RefSeq Version	XM_014106845.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris agouti signaling protein (ASIP), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014106845.1

ORF Insert Sequence:

ATGAATATCT TCCGCCTACT CCTGGCCACC CTGCTGGTCT CCCTGTGCTT CCTCACTGCC 
TACAGCCACC TGGCTGAGGA AAAGCCCAAG GATGACAGGA GCCTAAGGAG CAACTCCTCT 
GTGAACCTTT TGGATTTCCC TTCTGTCTCT ATTGTAGCAC TGAACAAGAA ATCCAAAAAG 
ATCAGCAGAA AAGAGGCGGA AAAGAAGAGA TCTTCTAAGA AAAAGGCTTC GATGAAGAAC 
GTGTCTCATC CCCGGCCCCC GCCACCCACC CCCTGCGTGG CCACTCGCAA CAGCTGCAAG 
TCCCCGGCGC CCGCCTGCTG TGACCCCTGC GCCTCCTGCC AGTGCCGCTT CTTCCGCAGC 
GCCTGCACCT GCCGCGTTCT CAGTCCCAGA TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASIP ( XM_014106843.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013962318.1 Canis lupus familiaris agouti signaling protein (ASIP), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe42634
Clone ID Related Accession (Same CDS sequence)	XM_014106843.1 , XM_022408819.1 , XM_014106845.1 , XM_014106843.2 , XM_022408820.1
Accession Version	XM_014106843.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	agouti-signaling protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014106843.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATATCT TCCGCCTACT CCTGGCCACC CTGCTGGTCT CCCTGTGCTT CCTCACTGCC 
TACAGCCACC TGGCTGAGGA AAAGCCCAAG GATGACAGGA GCCTAAGGAG CAACTCCTCT 
GTGAACCTTT TGGATTTCCC TTCTGTCTCT ATTGTAGCAC TGAACAAGAA ATCCAAAAAG 
ATCAGCAGAA AAGAGGCGGA AAAGAAGAGA TCTTCTAAGA AAAAGGCTTC GATGAAGAAC 
GTGTCTCATC CCCGGCCCCC GCCACCCACC CCCTGCGTGG CCACTCGCAA CAGCTGCAAG 
TCCCCGGCGC CCGCCTGCTG TGACCCCTGC GCCTCCTGCC AGTGCCGCTT CTTCCGCAGC 
GCCTGCACCT GCCGCGTTCT CAGTCCCAGA TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962318.1
CDS	182..577
Translation	MNIFRLLLATLLVSLCFLTAYSHLAEEKPKDDRSLRSNSSVNLLDFPSVSIVALNKKSKKISRKEAEKKRSSKKKASMKNVSHPRPPPPTPCVATRNSCKSPAPACCDPCASCQCRFFRSACTCRVLSPRC

Target ORF information:

RefSeq Version	XM_014106843.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris agouti signaling protein (ASIP), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014106843.2

ORF Insert Sequence:

ATGAATATCT TCCGCCTACT CCTGGCCACC CTGCTGGTCT CCCTGTGCTT CCTCACTGCC 
TACAGCCACC TGGCTGAGGA AAAGCCCAAG GATGACAGGA GCCTAAGGAG CAACTCCTCT 
GTGAACCTTT TGGATTTCCC TTCTGTCTCT ATTGTAGCAC TGAACAAGAA ATCCAAAAAG 
ATCAGCAGAA AAGAGGCGGA AAAGAAGAGA TCTTCTAAGA AAAAGGCTTC GATGAAGAAC 
GTGTCTCATC CCCGGCCCCC GCCACCCACC CCCTGCGTGG CCACTCGCAA CAGCTGCAAG 
TCCCCGGCGC CCGCCTGCTG TGACCCCTGC GCCTCCTGCC AGTGCCGCTT CTTCCGCAGC 
GCCTGCACCT GCCGCGTTCT CAGTCCCAGA TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASIP ( XM_022408820.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022264528.1 Canis lupus familiaris agouti signaling protein (ASIP), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe42634
Clone ID Related Accession (Same CDS sequence)	XM_014106843.1 , XM_022408819.1 , XM_014106845.1 , XM_014106843.2 , XM_022408820.1
Accession Version	XM_022408820.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	agouti-signaling protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATATCT TCCGCCTACT CCTGGCCACC CTGCTGGTCT CCCTGTGCTT CCTCACTGCC 
TACAGCCACC TGGCTGAGGA AAAGCCCAAG GATGACAGGA GCCTAAGGAG CAACTCCTCT 
GTGAACCTTT TGGATTTCCC TTCTGTCTCT ATTGTAGCAC TGAACAAGAA ATCCAAAAAG 
ATCAGCAGAA AAGAGGCGGA AAAGAAGAGA TCTTCTAAGA AAAAGGCTTC GATGAAGAAC 
GTGTCTCATC CCCGGCCCCC GCCACCCACC CCCTGCGTGG CCACTCGCAA CAGCTGCAAG 
TCCCCGGCGC CCGCCTGCTG TGACCCCTGC GCCTCCTGCC AGTGCCGCTT CTTCCGCAGC 
GCCTGCACCT GCCGCGTTCT CAGTCCCAGA TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022264528.1
CDS	587..982
Translation	MNIFRLLLATLLVSLCFLTAYSHLAEEKPKDDRSLRSNSSVNLLDFPSVSIVALNKKSKKISRKEAEKKRSSKKKASMKNVSHPRPPPPTPCVATRNSCKSPAPACCDPCASCQCRFFRSACTCRVLSPRC

Target ORF information:

RefSeq Version	XM_022408820.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris agouti signaling protein (ASIP), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022408820.1

ORF Insert Sequence:

ATGAATATCT TCCGCCTACT CCTGGCCACC CTGCTGGTCT CCCTGTGCTT CCTCACTGCC 
TACAGCCACC TGGCTGAGGA AAAGCCCAAG GATGACAGGA GCCTAAGGAG CAACTCCTCT 
GTGAACCTTT TGGATTTCCC TTCTGTCTCT ATTGTAGCAC TGAACAAGAA ATCCAAAAAG 
ATCAGCAGAA AAGAGGCGGA AAAGAAGAGA TCTTCTAAGA AAAAGGCTTC GATGAAGAAC 
GTGTCTCATC CCCGGCCCCC GCCACCCACC CCCTGCGTGG CCACTCGCAA CAGCTGCAAG 
TCCCCGGCGC CCGCCTGCTG TGACCCCTGC GCCTCCTGCC AGTGCCGCTT CTTCCGCAGC 
GCCTGCACCT GCCGCGTTCT CAGTCCCAGA TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Characterization of different 5'-untranslated exons of the ASIP gene in black-and-tan Doberman Pinscher and brindle Boxer dogs.
Animal genetics44(1)114-7(2013 Feb)
Ciampolini R,Cecchi F,Spaterna A,Bramante A,Bardet SM,Oulmouden A

A SINE insertion causes the black-and-tan and saddle tan phenotypes in domestic dogs.
The Journal of heredity102 Suppl 1S11-8(2011 Sep-Oct)
Dreger DL,Schmutz SM

Mutations in the melanocortin 1 receptor, ?-defensin103 and agouti signaling protein genes, and their association with coat color phenotypes in Akita-inu dogs.
The Journal of veterinary medical science73(7)853-8(2011 Jul)
Oguro-Okano M,Honda M,Yamazaki K,Okano K

Linkage and segregation analysis of black and brindle coat color in domestic dogs.
Genetics176(3)1679-89(2007 Jul)
Kerns JA,Cargill EJ,Clark LA,Candille SI,Berryere TG,Olivier M,Lust G,Todhunter RJ,Schmutz SM,Murphy KE,Barsh GS

Agouti sequence polymorphisms in coyotes, wolves and dogs suggest hybridization.
The Journal of heredity98(4)351-5(2007 Jul-Aug)
Schmutz SM,Berryere TG,Barta JL,Reddick KD,Schmutz JK

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ASIP cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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