TAF1 gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_022416443.1	XP_022272151.1	transcription initiation factor TFIID subunit 1 isoform X1
XM_005641473.3	XP_005641530.1	transcription initiation factor TFIID subunit 1 isoform X2
XM_003435561.4	XP_003435609.1	transcription initiation factor TFIID subunit 1 isoform X7
XM_005641475.2	XP_005641532.1	transcription initiation factor TFIID subunit 1 isoform X3
XM_014111608.1	XP_013967083.1	transcription initiation factor TFIID subunit 1 isoform X4
XM_844234.4	XP_849327.1	transcription initiation factor TFIID subunit 1 isoform X7
XM_014111608.2	XP_013967083.1	transcription initiation factor TFIID subunit 1 isoform X5
XM_005641473.2	XP_005641530.1	transcription initiation factor TFIID subunit 1 isoform X1
XM_005641474.3	XP_005641531.1	transcription initiation factor TFIID subunit 1 isoform X3
XM_005641477.3	XP_005641534.1	transcription initiation factor TFIID subunit 1 isoform X6
XM_005641474.2	XP_005641531.1	transcription initiation factor TFIID subunit 1 isoform X2
XM_005641477.2	XP_005641534.1	transcription initiation factor TFIID subunit 1 isoform X5
XM_003435561.3	XP_003435609.1	transcription initiation factor TFIID subunit 1 isoform X6
XM_005641475.3	XP_005641532.1	transcription initiation factor TFIID subunit 1 isoform X4
XM_844234.5	XP_849327.1	transcription initiation factor TFIID subunit 1 isoform X8

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe32526	XM_022416443.1 Latest version!	Canis lupus familiaris TATA-box binding protein associated factor 1 (TAF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe45585	XM_014111608.2 Latest version!	Canis lupus familiaris TATA-box binding protein associated factor 1 (TAF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe32527	XM_005641473.3 Latest version!	Canis lupus familiaris TATA-box binding protein associated factor 1 (TAF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe32528	XM_005641474.3 Latest version!	Canis lupus familiaris TATA-box binding protein associated factor 1 (TAF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe32529	XM_005641475.3 Latest version!	Canis lupus familiaris TATA-box binding protein associated factor 1 (TAF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe32531	XM_005641477.3 Latest version!	Canis lupus familiaris TATA-box binding protein associated factor 1 (TAF1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe32532	XM_003435561.4 Latest version!	Canis lupus familiaris TATA-box binding protein associated factor 1 (TAF1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe32533	XM_844234.5 Latest version!	Canis lupus familiaris TATA-box binding protein associated factor 1 (TAF1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
OCe45585	XM_014111608.1	Canis lupus familiaris TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa (TAF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe32527	XM_005641473.2	Canis lupus familiaris TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa (TAF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe32528	XM_005641474.2	Canis lupus familiaris TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa (TAF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe32531	XM_005641477.2	Canis lupus familiaris TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa (TAF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe32532	XM_003435561.3	Canis lupus familiaris TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa (TAF1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe32529	XM_005641475.2	Canis lupus familiaris TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa (TAF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe32533	XM_844234.4	Canis lupus familiaris TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa (TAF1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

TAF1 ( XM_022416443.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022272151.1 Canis lupus familiaris TATA-box binding protein associated factor 1 (TAF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe32526
Clone ID Related Accession (Same CDS sequence)	XM_022416443.1
Accession Version	XM_022416443.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5787bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	transcription initiation factor TFIID subunit 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACCTG GCTGGGATTT GCTGCTGCCT GTAGCCGCCG GAGACACTAC CAGCGCCATC 
ATGTCAGACA CGGACAGCGA TGAAGATTCT GCTGGAGGCG GCCCATTTTC TCTAACCGGT 
TTCCTTTTTG GCAACATCAA TGGAGCCGGG CAGCTGGAGG GGGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGTC TGGGCAGCCT GATCACTGAA 
CTCACGGCAA ACGAAGAATT GGCTGGGACC GATGGTGCCC TGGTAAATGA TGAAGGATGG 
ATCAGGAGTA CAGAAGATGC TGTGGACTAT TCAGACATCA CTGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ATCAGCAGAC AATGGGGAGC TTGCAGCCCC TTTGCCACTC AGATTATGAT 
GAAGATGACT ATGATGCCGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAATGAA GAAAGATAAG GACCAGGATG GTCTTACCGG TGTGTCTGAG 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCATCACAG 
GCAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA ACACGATGCC 
ACCAAGCTGT TGCCAAGTGT CACAGAACTT TTCCCGGAAT TTCGGCCTGG GAAGGTATTA 
CGCTTCCTTC GTCTTTTCGG ACCAGGGAAG AATGTCCCAT CTGTTTGGCG GAGTGCTCGG 
AGAAAGAGGA AGAAGAAGCA CCGTGAGCTG ATACAGGAAG AACAGATCCA GGAGGTGGAG 
TGCTCAGTAG AGTCCGAAGT CAACAAGAAG TCTTTATGGA ATTATGACTA TGCTCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATTACAA TGATGGCTCC TGTTGAATCA 
AAGTTTTCCC AGTCAACTGG AGATATAGAT AAAGTGACAG ATACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ATTATGGCTT CAAACTGAGA AAAATGGACC ATGAGCCTGT GATAAAATGT 
AGAATGATGG AGGACTTGAG GAAATTTGAG GAAAACAATG GCTCTGACCT ACTGGCTGAT 
GAAAACTTCC TGATGGTGAC ACAGCTGCAC TGGGAGGATG ATATCATCTG GGATGGGGAG 
GATGTCAAGC ACAAAGGAAC AAAGCCTCAG CGTGCAAGCC TGGCAGGCTG GCTTCCTTCC 
AGCATGACTA GGAATGCCAT GGCCTACAAT GTTCAGCAAG GTTTTGCAGC CACCCTGGAT 
GATGACAAAC CTTGGTACTC CATCTTTCCC ATTGACAACG AGGAGCTGGT ATATGGACGC 
TGGGAGGACA ATATCATTTG GGATGCTCAG GCCATGCCCA GGCTGTTGGA GCCTCCTGTT 
TTGACACTTG ATCCCAATGA TGAGAACCTT ATTTTGGAAA TTCCTGATGA AAAGGAAGAG 
GCCACTTCTA ACTCCCCCTC CAAGGAAAAT AAGAAAGAAT CATCTCTGAA GAAGAGCCGA 
ATTCTCTTAG GGAAAACAGG GGTCATCAAG GAGGAACCAC AGCAGAACAT GTCTCAGCCA 
GAAGTGAAAG ATCCATGGAA TCTCTCCAAT GATGAATATT ACTACCCCAA GCAACAGGGT 
CTTCGAGGCA CCTTTGGAGG AAATATTATC CAGCACTCAA TTCCTGCAGT GGAATTACGA 
CAGCCCTTCT TTCCTACCCA TATGGGGCCC ATCAAACTTC GGCAGTTCCA TCGTCCACCC 
CTGAAGAAAT ATTCCTTCGG GGCACTCTCT CAGCCAGGTC CCCACTCAGT CCAGCCCTTG 
CTGAAGCACA TCAAAAAGAA GGCTAAGATG AGAGAACAAG AGAGACAAGC TTCTGGTGGT 
GGAGAGATGT TTTTTATGCG CACACCTCAG GACCTCACAG GCAAAGATGG AGATCTTATT 
CTTGCAGAAT ACAGTGAGGA AAATGGACCA TTAATGATGC AGGTTGGCAT GGCAACCAAG 
ATAAAAAACT ATTATAAGCG GAAACCTGGA AAAGATCCTG GGGCCCCAGA TTGTAAATAT 
GGAGAAACTG TTTACTGTCA TACATCTCCT TTCCTAGGCT CTCTTCATCC TGGTCAATTA 
CTGCAGGCAT TTGAGAACAA TCTCTTTCGT GCCCCAATTT ATCTTCATAA GATGCCAGAA 
ACTGACTTCC TTATTATTCG GACAAGACAA GGTTACTACA TTCGGGAGTT AGTGGATATT 
TTTGTGGTCG GCCAGCAATG CCCCTTGTTT GAAGTTCCTG GGCCCAACTC CAAAAGAGCT 
AATACACATA TTCGAGACTT TCTCCAGGTT TTTATTTACC GCCTCTTCTG GAAGAGTAAA 
GATCGGCCAA GGCGGATCCG AATGGAAGAT ATAAAAAAAG CCTTTCCTTC TCACTCAGAA 
AGTAGCATCC GCAAGAGGCT AAAGCTCTGC GCTGACTTCA AACGCACAGG GATGGACTCA 
AACTGGTGGG TGCTAAAGTC AGATTTTCGT TTACCAACTG AAGAGGAGAT CAGAGCTATG 
GTGTCTCCAG AGCAGTGCTG TGCTTATTAT AGCATGATAG CTGCAGAGCA GCGACTAAAG 
GATGCTGGCT ATGGTGAGAA GTCCTTTTTT GCTCCAGAAG AAGAAAATGA GGAAGATTTC 
CAGATGAAGA TTGATGATGA AGTTCGCACT GCTCCTTGGA ATACCACAAG GGCTTTCATT 
GCTGCCATGA AGGGCAAGTG TCTCTTGGAG GTGACTGGGG TGGCAGATCC CACAGGGTGT 
GGTGAAGGAT TCTCCTATGT CAAGATTCCA AACAAACCAA CACAGCAGAA GGATGATAAG 
GAGCCTCAGC CAGTGAAGAA GACAGTGACA GGAACAGATG CAGATCTCCG TCGCCTCTCA 
CTGAAAAATG CCAAGCAGCT CCTACGTAAA TTTGGTGTGC CTGAGGAAGA GATTAAAAAG 
CTGTCCCGCT GGGAAGTGAT TGATGTGGTA CGCACAATGT CAACAGAGCA AGCCCGCTCT 
GGAGAGGGGC CCATGAGTAA ATTTGCCCGT GGATCAAGGT TTTCTGTGGC TGAGCATCAA 
GAGCGTTACA AAGAGGAATG TCAGCGCATC TTTGATCTAC AGAACAAGGT TTTGTCATCA 
ACTGAAATAT TGTCAACTGA CACAGACAGC AGCTCAGCCG AAGACAGTGA CTTTGAAGAA 
ATGGGAAAGA ACATTGAGAA CATGTTGCAG AATAAGAAAA CCAGCTCTCA GCTGTCACGT 
GAACGAGAGG AGCAGGAGCG GAAGGAACTG CAGCGGATGC TACTGGCAGC AGGTTCTGCA 
GCATCAGGAA ACAATCACAG AGATGATGAT ACAGCTTCTG TGACCAGCCT TAACTCTTCT 
GCCACTGGCC GTTGTCTCAA AATTTATCGC ACATTTCGAG ATGAAGAAGG GAAAGAGTAT 
GTTCGCTGTG AGACAGTCCG AAAGCCTGCT GTCATTGATG CCTATGTGCG CATACGGACC 
ACAAAAGATG AGGAATTCAT TCGAAAGTTT GCCCTTTTTG ATGAACAGCA TCGAGAAGAG 
ATGCGAAAGG AACGGCGGAG GATTCAAGAG CAACTTAGGC GGCTCAAGCG GAATCAGGAA 
AAGGAGAAGC TTAAGGGTCC CCCTGAGAAG AAGCCTAAAA AAATGAAGGA GCGTCCTGAT 
CTAAAACTGA AATGTGGAGC TTGTGGTGCC ATTGGGCACA TGAGGACAAA CAAGTTCTGC 
CCCCTCTACT ATCAAACAAA TGCTCCACCT TCCAACCCTG TTGCCATGAC AGAGGAGCAG 
GAGGAGGAGT TGGAAAAGAC AGTCATTCAT AATGATAATG AAGAACTTAT CAAGGTTGAA 
GGGACCAAGA TCGTCCTGGG AAAACAGCTA ATTGAGAGTG CAGATGAGGT TCGAAGAAAA 
TCTCTGGTTC TCAAGTTCCC TAAACAGCAA CTTCCTCCCA AGAAGAAACG GCGAGTTGGA 
ACCACTGTTC ACTGTGACTA CTTAAATAGA CCTCATAAAT CCATCCACCG GCGCCGGACA 
GACCCAATGG TGACATTGTC CTCCATCTTG GAGTCTATCA TCAATGACAT GAGAGATCTT 
CCAAATACCT ACCCTTTCCA CACTCCAGTC AATGCAAAGG TTGTAAAGGA CTACTACAAA 
ATCATCACTC GACCAATGGA CTTACAAACA CTCCGTGAAA ATGTGCGCAA ACGCCTTTAC 
CCATCTCGGG AAGAGTTCAG AGAACATTTG GAGCTCATTG TGAAAAATAG TGCAACCTAT 
AATGGTCCGA AGCACTCATT GACCCAGATC TCTCAATCTA TGCTGGATCT CTGTGATGAA 
AAACTCAAAG AGAAAGAAGA TAAATTGGCT CGTTTAGAGA AAGCTATCAA CCCCTTGCTG 
GATGATGATG ACCAAGTAGC ATTTTCTTTC ATTCTGGACA ACATTGTCAC CCAGAAAATG 
ATGGCAGTTC CAGATTCTTG GCCATTTCAT CACCCAGTTA ATAAGAAGTT TGTTCCAGAT 
TATTACAAAG TGATTATCAG TCCAATGGAT TTAGAGACTA TACGTAAGAA TATCTCCAAG 
CACAAGTACC AGAGTCGGGA GAGCTTTCTG GATGATGTCA ACCTTATTCT GGCCAACAGT 
GTTAAGTATA ATGGGCCTGA AAGTCAGTAT ACTAAGACTG CTCAGGAGAT TGTAAATGTC 
TGTTACCAGA CATTGACTGA GTATGATGAA CATTTGACTC AACTTGAGAA GGATATTTGT 
ACAGCTAAGG AAGCGGCTTT GGAGGAAGCA GAATTAGAAA GCTTGGACCC AATGACCCCA 
GGGCCTTATA CACCTCAGGC TAAGCCTCCT GATTTGTATG ACACCAACAC ATCCCTCAGT 
ATGTCCCGAG ATGCCTCTGT ATTTCAAGAT GAGAGCAATA TGTCTGTCCT GGATATTCCC 
ACTGCCACTC CAGAAAAGCA GGTGACACAG ATGCGCCAGG GCCGAGGTAG GCTGGGCGAG 
GAAGACTCTG ATGTAGATAT TGAAGGGTAT GATGATGAGG AGGAGGATGG GAAACCTAAG 
ACTCCAGCCC CAGAAGGTGA AGATGCAGAT GGTGATCTTG CAGATGAAGA GGAAGGAAGT 
ATGCAACAGC CTCAAGCCAG TGTCCTGTAT GAGGATTTGC TTATGTCTGA AGGAGAAGAT 
GATGAAGAAG ATGCTGGGAG TGATGAAGAA GGAGACAATC CTTTCTCTGC TATCCAACTG 
AGTGAAAGTG GAAGTGACTC TGATGTTGGA TCTAGTGGGA TAAGACCCAA ACAGCCCCGC 
ATGCTTCAGG AAAACACAAG GATGGGCATG GAAAATGAAG AAAGCATGAT GTCCTATGAG 
GGAGATGGTG GGGAGGCTTC TCATGGTTTG GAGGATAGCA ATATGAGTTA TGGGAGCTAT 
GAGGAGCCTG ATCCCAAGTC GAACACCCAA GATACAAGCT TCAGCAGCAT CGGTGGGTAT 
GAGGTATCAG AGGAAGAAGA AGATGAGGAG GAAGAGCAGC GCTCTGGGCC AAGTGTACTA 
AGCCAGGTCC ACCTGTCAGA GGATGAGGAG GACAGTGAGG ATTTCCACTC CATTGCTGGG 
GACAGTGATT TGGACTCTGA TGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022272151.1
CDS	104..5890
Translation	MGPGWDLLLPVAAGDTTSAIMSDTDSDEDSAGGGPFSLTGFLFGNINGAGQLEGESVLDDECKKHLAGLGALGLGSLITELTANEELAGTDGALVNDEGWIRSTEDAVDYSDITEVAEDESRRYQQTMGSLQPLCHSDYDEDDYDADCEDIDCKLMPPPPPPPGPMKKDKDQDGLTGVSEDGEGIILPSIIAPSSLASEKVDFSSSSDSESEMGPQEASQAESEDGKLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSARRKRKKKHRELIQEEQIQEVECSVESEVNKKSLWNYDYAPPPPPEQCLSDDEITMMAPVESKFSQSTGDIDKVTDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKLRKMDHEPVIKCRMMEDLRKFEENNGSDLLADENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDKPWYSIFPIDNEELVYGRWEDNIIWDAQAMPRLLEPPVLTLDPNDENLILEIPDEKEEATSNSPSKENKKESSLKKSRILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYYIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYYSMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEILSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMLLAAGSAASGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKPAVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIISPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNGPESQYTKTAQEIVNVCYQTLTEYDEHLTQLEKDICTAKEAALEEAELESLDPMTPGPYTPQAKPPDLYDTNTSLSMSRDASVFQDESNMSVLDIPTATPEKQVTQMRQGRGRLGEEDSDVDIEGYDDEEEDGKPKTPAPEGEDADGDLADEEEGSMQQPQASVLYEDLLMSEGEDDEEDAGSDEEGDNPFSAIQLSESGSDSDVGSSGIRPKQPRMLQENTRMGMENEESMMSYEGDGGEASHGLEDSNMSYGSYEEPDPKSNTQDTSFSSIGGYEVSEEEEDEEEEQRSGPSVLSQVHLSEDEEDSEDFHSIAGDSDLDSDE

Target ORF information:

RefSeq Version	XM_022416443.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris TATA-box binding protein associated factor 1 (TAF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022416443.1

ORF Insert Sequence:

ATGGGACCTG GCTGGGATTT GCTGCTGCCT GTAGCCGCCG GAGACACTAC CAGCGCCATC 
ATGTCAGACA CGGACAGCGA TGAAGATTCT GCTGGAGGCG GCCCATTTTC TCTAACCGGT 
TTCCTTTTTG GCAACATCAA TGGAGCCGGG CAGCTGGAGG GGGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGTC TGGGCAGCCT GATCACTGAA 
CTCACGGCAA ACGAAGAATT GGCTGGGACC GATGGTGCCC TGGTAAATGA TGAAGGATGG 
ATCAGGAGTA CAGAAGATGC TGTGGACTAT TCAGACATCA CTGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ATCAGCAGAC AATGGGGAGC TTGCAGCCCC TTTGCCACTC AGATTATGAT 
GAAGATGACT ATGATGCCGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAATGAA GAAAGATAAG GACCAGGATG GTCTTACCGG TGTGTCTGAG 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCATCACAG 
GCAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA ACACGATGCC 
ACCAAGCTGT TGCCAAGTGT CACAGAACTT TTCCCGGAAT TTCGGCCTGG GAAGGTATTA 
CGCTTCCTTC GTCTTTTCGG ACCAGGGAAG AATGTCCCAT CTGTTTGGCG GAGTGCTCGG 
AGAAAGAGGA AGAAGAAGCA CCGTGAGCTG ATACAGGAAG AACAGATCCA GGAGGTGGAG 
TGCTCAGTAG AGTCCGAAGT CAACAAGAAG TCTTTATGGA ATTATGACTA TGCTCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATTACAA TGATGGCTCC TGTTGAATCA 
AAGTTTTCCC AGTCAACTGG AGATATAGAT AAAGTGACAG ATACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ATTATGGCTT CAAACTGAGA AAAATGGACC ATGAGCCTGT GATAAAATGT 
AGAATGATGG AGGACTTGAG GAAATTTGAG GAAAACAATG GCTCTGACCT ACTGGCTGAT 
GAAAACTTCC TGATGGTGAC ACAGCTGCAC TGGGAGGATG ATATCATCTG GGATGGGGAG 
GATGTCAAGC ACAAAGGAAC AAAGCCTCAG CGTGCAAGCC TGGCAGGCTG GCTTCCTTCC 
AGCATGACTA GGAATGCCAT GGCCTACAAT GTTCAGCAAG GTTTTGCAGC CACCCTGGAT 
GATGACAAAC CTTGGTACTC CATCTTTCCC ATTGACAACG AGGAGCTGGT ATATGGACGC 
TGGGAGGACA ATATCATTTG GGATGCTCAG GCCATGCCCA GGCTGTTGGA GCCTCCTGTT 
TTGACACTTG ATCCCAATGA TGAGAACCTT ATTTTGGAAA TTCCTGATGA AAAGGAAGAG 
GCCACTTCTA ACTCCCCCTC CAAGGAAAAT AAGAAAGAAT CATCTCTGAA GAAGAGCCGA 
ATTCTCTTAG GGAAAACAGG GGTCATCAAG GAGGAACCAC AGCAGAACAT GTCTCAGCCA 
GAAGTGAAAG ATCCATGGAA TCTCTCCAAT GATGAATATT ACTACCCCAA GCAACAGGGT 
CTTCGAGGCA CCTTTGGAGG AAATATTATC CAGCACTCAA TTCCTGCAGT GGAATTACGA 
CAGCCCTTCT TTCCTACCCA TATGGGGCCC ATCAAACTTC GGCAGTTCCA TCGTCCACCC 
CTGAAGAAAT ATTCCTTCGG GGCACTCTCT CAGCCAGGTC CCCACTCAGT CCAGCCCTTG 
CTGAAGCACA TCAAAAAGAA GGCTAAGATG AGAGAACAAG AGAGACAAGC TTCTGGTGGT 
GGAGAGATGT TTTTTATGCG CACACCTCAG GACCTCACAG GCAAAGATGG AGATCTTATT 
CTTGCAGAAT ACAGTGAGGA AAATGGACCA TTAATGATGC AGGTTGGCAT GGCAACCAAG 
ATAAAAAACT ATTATAAGCG GAAACCTGGA AAAGATCCTG GGGCCCCAGA TTGTAAATAT 
GGAGAAACTG TTTACTGTCA TACATCTCCT TTCCTAGGCT CTCTTCATCC TGGTCAATTA 
CTGCAGGCAT TTGAGAACAA TCTCTTTCGT GCCCCAATTT ATCTTCATAA GATGCCAGAA 
ACTGACTTCC TTATTATTCG GACAAGACAA GGTTACTACA TTCGGGAGTT AGTGGATATT 
TTTGTGGTCG GCCAGCAATG CCCCTTGTTT GAAGTTCCTG GGCCCAACTC CAAAAGAGCT 
AATACACATA TTCGAGACTT TCTCCAGGTT TTTATTTACC GCCTCTTCTG GAAGAGTAAA 
GATCGGCCAA GGCGGATCCG AATGGAAGAT ATAAAAAAAG CCTTTCCTTC TCACTCAGAA 
AGTAGCATCC GCAAGAGGCT AAAGCTCTGC GCTGACTTCA AACGCACAGG GATGGACTCA 
AACTGGTGGG TGCTAAAGTC AGATTTTCGT TTACCAACTG AAGAGGAGAT CAGAGCTATG 
GTGTCTCCAG AGCAGTGCTG TGCTTATTAT AGCATGATAG CTGCAGAGCA GCGACTAAAG 
GATGCTGGCT ATGGTGAGAA GTCCTTTTTT GCTCCAGAAG AAGAAAATGA GGAAGATTTC 
CAGATGAAGA TTGATGATGA AGTTCGCACT GCTCCTTGGA ATACCACAAG GGCTTTCATT 
GCTGCCATGA AGGGCAAGTG TCTCTTGGAG GTGACTGGGG TGGCAGATCC CACAGGGTGT 
GGTGAAGGAT TCTCCTATGT CAAGATTCCA AACAAACCAA CACAGCAGAA GGATGATAAG 
GAGCCTCAGC CAGTGAAGAA GACAGTGACA GGAACAGATG CAGATCTCCG TCGCCTCTCA 
CTGAAAAATG CCAAGCAGCT CCTACGTAAA TTTGGTGTGC CTGAGGAAGA GATTAAAAAG 
CTGTCCCGCT GGGAAGTGAT TGATGTGGTA CGCACAATGT CAACAGAGCA AGCCCGCTCT 
GGAGAGGGGC CCATGAGTAA ATTTGCCCGT GGATCAAGGT TTTCTGTGGC TGAGCATCAA 
GAGCGTTACA AAGAGGAATG TCAGCGCATC TTTGATCTAC AGAACAAGGT TTTGTCATCA 
ACTGAAATAT TGTCAACTGA CACAGACAGC AGCTCAGCCG AAGACAGTGA CTTTGAAGAA 
ATGGGAAAGA ACATTGAGAA CATGTTGCAG AATAAGAAAA CCAGCTCTCA GCTGTCACGT 
GAACGAGAGG AGCAGGAGCG GAAGGAACTG CAGCGGATGC TACTGGCAGC AGGTTCTGCA 
GCATCAGGAA ACAATCACAG AGATGATGAT ACAGCTTCTG TGACCAGCCT TAACTCTTCT 
GCCACTGGCC GTTGTCTCAA AATTTATCGC ACATTTCGAG ATGAAGAAGG GAAAGAGTAT 
GTTCGCTGTG AGACAGTCCG AAAGCCTGCT GTCATTGATG CCTATGTGCG CATACGGACC 
ACAAAAGATG AGGAATTCAT TCGAAAGTTT GCCCTTTTTG ATGAACAGCA TCGAGAAGAG 
ATGCGAAAGG AACGGCGGAG GATTCAAGAG CAACTTAGGC GGCTCAAGCG GAATCAGGAA 
AAGGAGAAGC TTAAGGGTCC CCCTGAGAAG AAGCCTAAAA AAATGAAGGA GCGTCCTGAT 
CTAAAACTGA AATGTGGAGC TTGTGGTGCC ATTGGGCACA TGAGGACAAA CAAGTTCTGC 
CCCCTCTACT ATCAAACAAA TGCTCCACCT TCCAACCCTG TTGCCATGAC AGAGGAGCAG 
GAGGAGGAGT TGGAAAAGAC AGTCATTCAT AATGATAATG AAGAACTTAT CAAGGTTGAA 
GGGACCAAGA TCGTCCTGGG AAAACAGCTA ATTGAGAGTG CAGATGAGGT TCGAAGAAAA 
TCTCTGGTTC TCAAGTTCCC TAAACAGCAA CTTCCTCCCA AGAAGAAACG GCGAGTTGGA 
ACCACTGTTC ACTGTGACTA CTTAAATAGA CCTCATAAAT CCATCCACCG GCGCCGGACA 
GACCCAATGG TGACATTGTC CTCCATCTTG GAGTCTATCA TCAATGACAT GAGAGATCTT 
CCAAATACCT ACCCTTTCCA CACTCCAGTC AATGCAAAGG TTGTAAAGGA CTACTACAAA 
ATCATCACTC GACCAATGGA CTTACAAACA CTCCGTGAAA ATGTGCGCAA ACGCCTTTAC 
CCATCTCGGG AAGAGTTCAG AGAACATTTG GAGCTCATTG TGAAAAATAG TGCAACCTAT 
AATGGTCCGA AGCACTCATT GACCCAGATC TCTCAATCTA TGCTGGATCT CTGTGATGAA 
AAACTCAAAG AGAAAGAAGA TAAATTGGCT CGTTTAGAGA AAGCTATCAA CCCCTTGCTG 
GATGATGATG ACCAAGTAGC ATTTTCTTTC ATTCTGGACA ACATTGTCAC CCAGAAAATG 
ATGGCAGTTC CAGATTCTTG GCCATTTCAT CACCCAGTTA ATAAGAAGTT TGTTCCAGAT 
TATTACAAAG TGATTATCAG TCCAATGGAT TTAGAGACTA TACGTAAGAA TATCTCCAAG 
CACAAGTACC AGAGTCGGGA GAGCTTTCTG GATGATGTCA ACCTTATTCT GGCCAACAGT 
GTTAAGTATA ATGGGCCTGA AAGTCAGTAT ACTAAGACTG CTCAGGAGAT TGTAAATGTC 
TGTTACCAGA CATTGACTGA GTATGATGAA CATTTGACTC AACTTGAGAA GGATATTTGT 
ACAGCTAAGG AAGCGGCTTT GGAGGAAGCA GAATTAGAAA GCTTGGACCC AATGACCCCA 
GGGCCTTATA CACCTCAGGC TAAGCCTCCT GATTTGTATG ACACCAACAC ATCCCTCAGT 
ATGTCCCGAG ATGCCTCTGT ATTTCAAGAT GAGAGCAATA TGTCTGTCCT GGATATTCCC 
ACTGCCACTC CAGAAAAGCA GGTGACACAG ATGCGCCAGG GCCGAGGTAG GCTGGGCGAG 
GAAGACTCTG ATGTAGATAT TGAAGGGTAT GATGATGAGG AGGAGGATGG GAAACCTAAG 
ACTCCAGCCC CAGAAGGTGA AGATGCAGAT GGTGATCTTG CAGATGAAGA GGAAGGAAGT 
ATGCAACAGC CTCAAGCCAG TGTCCTGTAT GAGGATTTGC TTATGTCTGA AGGAGAAGAT 
GATGAAGAAG ATGCTGGGAG TGATGAAGAA GGAGACAATC CTTTCTCTGC TATCCAACTG 
AGTGAAAGTG GAAGTGACTC TGATGTTGGA TCTAGTGGGA TAAGACCCAA ACAGCCCCGC 
ATGCTTCAGG AAAACACAAG GATGGGCATG GAAAATGAAG AAAGCATGAT GTCCTATGAG 
GGAGATGGTG GGGAGGCTTC TCATGGTTTG GAGGATAGCA ATATGAGTTA TGGGAGCTAT 
GAGGAGCCTG ATCCCAAGTC GAACACCCAA GATACAAGCT TCAGCAGCAT CGGTGGGTAT 
GAGGTATCAG AGGAAGAAGA AGATGAGGAG GAAGAGCAGC GCTCTGGGCC AAGTGTACTA 
AGCCAGGTCC ACCTGTCAGA GGATGAGGAG GACAGTGAGG ATTTCCACTC CATTGCTGGG 
GACAGTGATT TGGACTCTGA TGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAF1 ( XM_014111608.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013967083.1 Canis lupus familiaris TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa (TAF1), transcript variant X4, mRNA.

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CloneID	OCe45585
Clone ID Related Accession (Same CDS sequence)	XM_014111608.1 , XM_014111608.2
Accession Version	XM_014111608.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	transcription initiation factor TFIID subunit 1 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACCTG GCTGGGATTT GCTGCTGCCT GTAGCCGCCG GAGACACTAC CAGCGCCATC 
ATGTCAGACA CGGACAGCGA TGAAGATTCT GCTGGAGGCG GCCCATTTTC TCTAACCGGT 
TTCCTTTTTG GCAACATCAA TGGAGCCGGG CAGCTGGAGG GGGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGTC TGGGCAGCCT GATCACTGAA 
CTCACGGCAA ACGAAGAATT GGCTGGGACC GATGGTGCCC TGGTAAATGA TGAAGGATGG 
ATCAGGAGTA CAGAAGATGC TGTGGACTAT TCAGACATCA CTGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ATCAGCAGAC AATGGGGAGC TTGCAGCCCC TTTGCCACTC AGATTATGAT 
GAAGATGACT ATGATGCCGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAATGAA GAAAGATAAG GACCAGGATG GTCTTACCGG TGTGTCTGAG 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCATCACAG 
GCAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA ACACGATGCC 
ACCAAGCTGT TGCCAAGTGT CACAGAACTT TTCCCGGAAT TTCGGCCTGG GAAGGTATTA 
CGCTTCCTTC GTCTTTTCGG ACCAGGGAAG AATGTCCCAT CTGTTTGGCG GAGTGCTCGG 
AGAAAGAGGA AGAAGAAGCA CCGTGAGCTG ATACAGGAAG AACAGATCCA GGAGATTACA 
ATGATGGCTC CTGTTGAATC AAAGTTTTCC CAGTCAACTG GAGATATAGA TAAAGTGACA 
GATACCAAAC CAAGAGTGGC TGAGTGGCGT TATGGGCCTG CTCGACTGTG GTATGATATG 
CTGGGTGTCC CTGAAGATGG CAGTGGGTTT GATTATGGCT TCAAACTGAG AAAAATGGAC 
CATGAGCCTG TGATAAAATG TAGAATGATG GAGGACTTGA GGAAATTTGA GGAAAACAAT 
GGCTCTGACC TACTGGCTGA TGAAAACTTC CTGATGGTGA CACAGCTGCA CTGGGAGGAT 
GATATCATCT GGGATGGGGA GGATGTCAAG CACAAAGGAA CAAAGCCTCA GCGTGCAAGC 
CTGGCAGGCT GGCTTCCTTC CAGCATGACT AGGAATGCCA TGGCCTACAA TGTTCAGCAA 
GGTTTTGCAG CCACCCTGGA TGATGACAAA CCTTGGTACT CCATCTTTCC CATTGACAAC 
GAGGAGCTGG TATATGGACG CTGGGAGGAC AATATCATTT GGGATGCTCA GGCCATGCCC 
AGGCTGTTGG AGCCTCCTGT TTTGACACTT GATCCCAATG ATGAGAACCT TATTTTGGAA 
ATTCCTGATG AAAAGGAAGA GGCCACTTCT AACTCCCCCT CCAAGGAAAA TAAGAAAGAA 
TCATCTCTGA AGAAGAGCCG AATTCTCTTA GGGAAAACAG GGGTCATCAA GGAGGAACCA 
CAGCAGAACA TGTCTCAGCC AGAAGTGAAA GATCCATGGA ATCTCTCCAA TGATGAATAT 
TACTACCCCA AGCAACAGGG TCTTCGAGGC ACCTTTGGAG GAAATATTAT CCAGCACTCA 
ATTCCTGCAG TGGAATTACG ACAGCCCTTC TTTCCTACCC ATATGGGGCC CATCAAACTT 
CGGCAGTTCC ATCGTCCACC CCTGAAGAAA TATTCCTTCG GGGCACTCTC TCAGCCAGGT 
CCCCACTCAG TCCAGCCCTT GCTGAAGCAC ATCAAAAAGA AGGCTAAGAT GAGAGAACAA 
GAGAGACAAG CTTCTGGTGG TGGAGAGATG TTTTTTATGC GCACACCTCA GGACCTCACA 
GGCAAAGATG GAGATCTTAT TCTTGCAGAA TACAGTGAGG AAAATGGACC ATTAATGATG 
CAGGTTGGCA TGGCAACCAA GATAAAAAAC TATTATAAGC GGAAACCTGG AAAAGATCCT 
GGGGCCCCAG ATTGTAAATA TGGAGAAACT GTTTACTGTC ATACATCTCC TTTCCTAGGC 
TCTCTTCATC CTGGTCAATT ACTGCAGGCA TTTGAGAACA ATCTCTTTCG TGCCCCAATT 
TATCTTCATA AGATGCCAGA AACTGACTTC CTTATTATTC GGACAAGACA AGGTTACTAC 
ATTCGGGAGT TAGTGGATAT TTTTGTGGTC GGCCAGCAAT GCCCCTTGTT TGAAGTTCCT 
GGGCCCAACT CCAAAAGAGC TAATACACAT ATTCGAGACT TTCTCCAGGT TTTTATTTAC 
CGCCTCTTCT GGAAGAGTAA AGATCGGCCA AGGCGGATCC GAATGGAAGA TATAAAAAAA 
GCCTTTCCTT CTCACTCAGA AAGTAGCATC CGCAAGAGGC TAAAGCTCTG CGCTGACTTC 
AAACGCACAG GGATGGACTC AAACTGGTGG GTGCTAAAGT CAGATTTTCG TTTACCAACT 
GAAGAGGAGA TCAGAGCTAT GGTGTCTCCA GAGCAGTGCT GTGCTTATTA TAGCATGATA 
GCTGCAGAGC AGCGACTAAA GGATGCTGGC TATGGTGAGA AGTCCTTTTT TGCTCCAGAA 
GAAGAAAATG AGGAAGATTT CCAGATGAAG ATTGATGATG AAGTTCGCAC TGCTCCTTGG 
AATACCACAA GGGCTTTCAT TGCTGCCATG AAGGGCAAGT GTCTCTTGGA GGTGACTGGG 
GTGGCAGATC CCACAGGGTG TGGTGAAGGA TTCTCCTATG TCAAGATTCC AAACAAACCA 
ACACAGCAGA AGGATGATAA GGAGCCTCAG CCAGTGAAGA AGACAGTGAC AGGAACAGAT 
GCAGATCTCC GTCGCCTCTC ACTGAAAAAT GCCAAGCAGC TCCTACGTAA ATTTGGTGTG 
CCTGAGGAAG AGATTAAAAA GCTGTCCCGC TGGGAAGTGA TTGATGTGGT ACGCACAATG 
TCAACAGAGC AAGCCCGCTC TGGAGAGGGG CCCATGAGTA AATTTGCCCG TGGATCAAGG 
TTTTCTGTGG CTGAGCATCA AGAGCGTTAC AAAGAGGAAT GTCAGCGCAT CTTTGATCTA 
CAGAACAAGG TTTTGTCATC AACTGAAATA TTGTCAACTG ACACAGACAG CAGCTCAGCC 
GAAGACAGTG ACTTTGAAGA AATGGGAAAG AACATTGAGA ACATGTTGCA GAATAAGAAA 
ACCAGCTCTC AGCTGTCACG TGAACGAGAG GAGCAGGAGC GGAAGGAACT GCAGCGGATG 
CTACTGGCAG CAGGTTCTGC AGCATCAGGA AACAATCACA GAGATGATGA TACAGCTTCT 
GTGACCAGCC TTAACTCTTC TGCCACTGGC CGTTGTCTCA AAATTTATCG CACATTTCGA 
GATGAAGAAG GGAAAGAGTA TGTTCGCTGT GAGACAGTCC GAAAGCCTGC TGTCATTGAT 
GCCTATGTGC GCATACGGAC CACAAAAGAT GAGGAATTCA TTCGAAAGTT TGCCCTTTTT 
GATGAACAGC ATCGAGAAGA GATGCGAAAG GAACGGCGGA GGATTCAAGA GCAACTTAGG 
CGGCTCAAGC GGAATCAGGA AAAGGAGAAG CTTAAGGGTC CCCCTGAGAA GAAGCCTAAA 
AAAATGAAGG AGCGTCCTGA TCTAAAACTG AAATGTGGAG CTTGTGGTGC CATTGGGCAC 
ATGAGGACAA ACAAGTTCTG CCCCCTCTAC TATCAAACAA ATGCTCCACC TTCCAACCCT 
GTTGCCATGA CAGAGGAGCA GGAGGAGGAG TTGGAAAAGA CAGTCATTCA TAATGATAAT 
GAAGAACTTA TCAAGGTTGA AGGGACCAAG ATCGTCCTGG GAAAACAGCT AATTGAGAGT 
GCAGATGAGG TTCGAAGAAA ATCTCTGGTT CTCAAGTTCC CTAAACAGCA ACTTCCTCCC 
AAGAAGAAAC GGCGAGTTGG AACCACTGTT CACTGTGACT ACTTAAATAG ACCTCATAAA 
TCCATCCACC GGCGCCGGAC AGACCCAATG GTGACATTGT CCTCCATCTT GGAGTCTATC 
ATCAATGACA TGAGAGATCT TCCAAATACC TACCCTTTCC ACACTCCAGT CAATGCAAAG 
GTTGTAAAGG ACTACTACAA AATCATCACT CGACCAATGG ACTTACAAAC ACTCCGTGAA 
AATGTGCGCA AACGCCTTTA CCCATCTCGG GAAGAGTTCA GAGAACATTT GGAGCTCATT 
GTGAAAAATA GTGCAACCTA TAATGGTCCG AAGCACTCAT TGACCCAGAT CTCTCAATCT 
ATGCTGGATC TCTGTGATGA AAAACTCAAA GAGAAAGAAG ATAAATTGGC TCGTTTAGAG 
AAAGCTATCA ACCCCTTGCT GGATGATGAT GACCAAGTAG CATTTTCTTT CATTCTGGAC 
AACATTGTCA CCCAGAAAAT GATGGCAGTT CCAGATTCTT GGCCATTTCA TCACCCAGTT 
AATAAGAAGT TTGTTCCAGA TTATTACAAA GTGATTATCA GTCCAATGGA TTTAGAGACT 
ATACGTAAGA ATATCTCCAA GCACAAGTAC CAGAGTCGGG AGAGCTTTCT GGATGATGTC 
AACCTTATTC TGGCCAACAG TGTTAAGTAT AATGGGCCTG AAAGTCAGTA TACTAAGACT 
GCTCAGGAGA TTGTAAATGT CTGTTACCAG ACATTGACTG AGTATGATGA ACATTTGACT 
CAACTTGAGA AGGATATTTG TACAGCTAAG GAAGCGGCTT TGGAGGAAGC AGAATTAGAA 
AGCTTGGACC CAATGACCCC AGGGCCTTAT ACACCTCAGG CTAAGCCTCC TGATTTGTAT 
GACACCAACA CATCCCTCAG TATGTCCCGA GATGCCTCTG TATTTCAAGA TGAGAGCAAT 
ATGTCTGTCC TGGATATTCC CACTGCCACT CCAGAAAAGC AGGTGACACA GATGCGCCAG 
GGCCGAGGTA GGCTGGGCGA GGAAGACTCT GATGTAGATA TTGAAGGGTA TGATGATGAG 
GAGGAGGATG GGAAACCTAA GACTCCAGCC CCAGAAGGTG AAGATGCAGA TGGTGATCTT 
GCAGATGAAG AGGAAGGAAG TATGCAACAG CCTCAAGCCA GTGTCCTGTA TGAGGATTTG 
CTTATGTCTG AAGGAGAAGA TGATGAAGAA GATGCTGGGA GTGATGAAGA AGGAGACAAT 
CCTTTCTCTG CTATCCAACT GAGTGAAAGT GGAAGTGACT CTGATGTTGG ATCTAGTGGG 
ATAAGACCCA AACAGCCCCG CATGCTTCAG GAAAACACAA GGATGGGCAT GGAAAATGAA 
GAAAGCATGA TGTCCTATGA GGGAGATGGT GGGGAGGCTT CTCATGGTTT GGAGGATAGC 
AATATGAGTT ATGGGAGCTA TGAGGAGCCT GATCCCAAGT CGAACACCCA AGATACAAGC 
TTCAGCAGCA TCGGTGGGTA TGAGGTATCA GAGGAAGAAG AAGATGAGGA GGAAGAGCAG 
CGCTCTGGGC CAAGTGTACT AAGCCAGGTC CACCTGTCAG AGGATGAGGA GGACAGTGAG 
GATTTCCACT CCATTGCTGG GGACAGTGAT TTGGACTCTG ATGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967083.1
CDS	93..5780
Translation	MGPGWDLLLPVAAGDTTSAIMSDTDSDEDSAGGGPFSLTGFLFGNINGAGQLEGESVLDDECKKHLAGLGALGLGSLITELTANEELAGTDGALVNDEGWIRSTEDAVDYSDITEVAEDESRRYQQTMGSLQPLCHSDYDEDDYDADCEDIDCKLMPPPPPPPGPMKKDKDQDGLTGVSEDGEGIILPSIIAPSSLASEKVDFSSSSDSESEMGPQEASQAESEDGKLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSARRKRKKKHRELIQEEQIQEITMMAPVESKFSQSTGDIDKVTDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKLRKMDHEPVIKCRMMEDLRKFEENNGSDLLADENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDKPWYSIFPIDNEELVYGRWEDNIIWDAQAMPRLLEPPVLTLDPNDENLILEIPDEKEEATSNSPSKENKKESSLKKSRILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYYIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYYSMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEILSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMLLAAGSAASGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKPAVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIISPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNGPESQYTKTAQEIVNVCYQTLTEYDEHLTQLEKDICTAKEAALEEAELESLDPMTPGPYTPQAKPPDLYDTNTSLSMSRDASVFQDESNMSVLDIPTATPEKQVTQMRQGRGRLGEEDSDVDIEGYDDEEEDGKPKTPAPEGEDADGDLADEEEGSMQQPQASVLYEDLLMSEGEDDEEDAGSDEEGDNPFSAIQLSESGSDSDVGSSGIRPKQPRMLQENTRMGMENEESMMSYEGDGGEASHGLEDSNMSYGSYEEPDPKSNTQDTSFSSIGGYEVSEEEEDEEEEQRSGPSVLSQVHLSEDEEDSEDFHSIAGDSDLDSDE

Target ORF information:

RefSeq Version	XM_014111608.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa (TAF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111608.1

ORF Insert Sequence:

ATGGGACCTG GCTGGGATTT GCTGCTGCCT GTAGCCGCCG GAGACACTAC CAGCGCCATC 
ATGTCAGACA CGGACAGCGA TGAAGATTCT GCTGGAGGCG GCCCATTTTC TCTAACCGGT 
TTCCTTTTTG GCAACATCAA TGGAGCCGGG CAGCTGGAGG GGGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGTC TGGGCAGCCT GATCACTGAA 
CTCACGGCAA ACGAAGAATT GGCTGGGACC GATGGTGCCC TGGTAAATGA TGAAGGATGG 
ATCAGGAGTA CAGAAGATGC TGTGGACTAT TCAGACATCA CTGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ATCAGCAGAC AATGGGGAGC TTGCAGCCCC TTTGCCACTC AGATTATGAT 
GAAGATGACT ATGATGCCGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAATGAA GAAAGATAAG GACCAGGATG GTCTTACCGG TGTGTCTGAG 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCATCACAG 
GCAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA ACACGATGCC 
ACCAAGCTGT TGCCAAGTGT CACAGAACTT TTCCCGGAAT TTCGGCCTGG GAAGGTATTA 
CGCTTCCTTC GTCTTTTCGG ACCAGGGAAG AATGTCCCAT CTGTTTGGCG GAGTGCTCGG 
AGAAAGAGGA AGAAGAAGCA CCGTGAGCTG ATACAGGAAG AACAGATCCA GGAGATTACA 
ATGATGGCTC CTGTTGAATC AAAGTTTTCC CAGTCAACTG GAGATATAGA TAAAGTGACA 
GATACCAAAC CAAGAGTGGC TGAGTGGCGT TATGGGCCTG CTCGACTGTG GTATGATATG 
CTGGGTGTCC CTGAAGATGG CAGTGGGTTT GATTATGGCT TCAAACTGAG AAAAATGGAC 
CATGAGCCTG TGATAAAATG TAGAATGATG GAGGACTTGA GGAAATTTGA GGAAAACAAT 
GGCTCTGACC TACTGGCTGA TGAAAACTTC CTGATGGTGA CACAGCTGCA CTGGGAGGAT 
GATATCATCT GGGATGGGGA GGATGTCAAG CACAAAGGAA CAAAGCCTCA GCGTGCAAGC 
CTGGCAGGCT GGCTTCCTTC CAGCATGACT AGGAATGCCA TGGCCTACAA TGTTCAGCAA 
GGTTTTGCAG CCACCCTGGA TGATGACAAA CCTTGGTACT CCATCTTTCC CATTGACAAC 
GAGGAGCTGG TATATGGACG CTGGGAGGAC AATATCATTT GGGATGCTCA GGCCATGCCC 
AGGCTGTTGG AGCCTCCTGT TTTGACACTT GATCCCAATG ATGAGAACCT TATTTTGGAA 
ATTCCTGATG AAAAGGAAGA GGCCACTTCT AACTCCCCCT CCAAGGAAAA TAAGAAAGAA 
TCATCTCTGA AGAAGAGCCG AATTCTCTTA GGGAAAACAG GGGTCATCAA GGAGGAACCA 
CAGCAGAACA TGTCTCAGCC AGAAGTGAAA GATCCATGGA ATCTCTCCAA TGATGAATAT 
TACTACCCCA AGCAACAGGG TCTTCGAGGC ACCTTTGGAG GAAATATTAT CCAGCACTCA 
ATTCCTGCAG TGGAATTACG ACAGCCCTTC TTTCCTACCC ATATGGGGCC CATCAAACTT 
CGGCAGTTCC ATCGTCCACC CCTGAAGAAA TATTCCTTCG GGGCACTCTC TCAGCCAGGT 
CCCCACTCAG TCCAGCCCTT GCTGAAGCAC ATCAAAAAGA AGGCTAAGAT GAGAGAACAA 
GAGAGACAAG CTTCTGGTGG TGGAGAGATG TTTTTTATGC GCACACCTCA GGACCTCACA 
GGCAAAGATG GAGATCTTAT TCTTGCAGAA TACAGTGAGG AAAATGGACC ATTAATGATG 
CAGGTTGGCA TGGCAACCAA GATAAAAAAC TATTATAAGC GGAAACCTGG AAAAGATCCT 
GGGGCCCCAG ATTGTAAATA TGGAGAAACT GTTTACTGTC ATACATCTCC TTTCCTAGGC 
TCTCTTCATC CTGGTCAATT ACTGCAGGCA TTTGAGAACA ATCTCTTTCG TGCCCCAATT 
TATCTTCATA AGATGCCAGA AACTGACTTC CTTATTATTC GGACAAGACA AGGTTACTAC 
ATTCGGGAGT TAGTGGATAT TTTTGTGGTC GGCCAGCAAT GCCCCTTGTT TGAAGTTCCT 
GGGCCCAACT CCAAAAGAGC TAATACACAT ATTCGAGACT TTCTCCAGGT TTTTATTTAC 
CGCCTCTTCT GGAAGAGTAA AGATCGGCCA AGGCGGATCC GAATGGAAGA TATAAAAAAA 
GCCTTTCCTT CTCACTCAGA AAGTAGCATC CGCAAGAGGC TAAAGCTCTG CGCTGACTTC 
AAACGCACAG GGATGGACTC AAACTGGTGG GTGCTAAAGT CAGATTTTCG TTTACCAACT 
GAAGAGGAGA TCAGAGCTAT GGTGTCTCCA GAGCAGTGCT GTGCTTATTA TAGCATGATA 
GCTGCAGAGC AGCGACTAAA GGATGCTGGC TATGGTGAGA AGTCCTTTTT TGCTCCAGAA 
GAAGAAAATG AGGAAGATTT CCAGATGAAG ATTGATGATG AAGTTCGCAC TGCTCCTTGG 
AATACCACAA GGGCTTTCAT TGCTGCCATG AAGGGCAAGT GTCTCTTGGA GGTGACTGGG 
GTGGCAGATC CCACAGGGTG TGGTGAAGGA TTCTCCTATG TCAAGATTCC AAACAAACCA 
ACACAGCAGA AGGATGATAA GGAGCCTCAG CCAGTGAAGA AGACAGTGAC AGGAACAGAT 
GCAGATCTCC GTCGCCTCTC ACTGAAAAAT GCCAAGCAGC TCCTACGTAA ATTTGGTGTG 
CCTGAGGAAG AGATTAAAAA GCTGTCCCGC TGGGAAGTGA TTGATGTGGT ACGCACAATG 
TCAACAGAGC AAGCCCGCTC TGGAGAGGGG CCCATGAGTA AATTTGCCCG TGGATCAAGG 
TTTTCTGTGG CTGAGCATCA AGAGCGTTAC AAAGAGGAAT GTCAGCGCAT CTTTGATCTA 
CAGAACAAGG TTTTGTCATC AACTGAAATA TTGTCAACTG ACACAGACAG CAGCTCAGCC 
GAAGACAGTG ACTTTGAAGA AATGGGAAAG AACATTGAGA ACATGTTGCA GAATAAGAAA 
ACCAGCTCTC AGCTGTCACG TGAACGAGAG GAGCAGGAGC GGAAGGAACT GCAGCGGATG 
CTACTGGCAG CAGGTTCTGC AGCATCAGGA AACAATCACA GAGATGATGA TACAGCTTCT 
GTGACCAGCC TTAACTCTTC TGCCACTGGC CGTTGTCTCA AAATTTATCG CACATTTCGA 
GATGAAGAAG GGAAAGAGTA TGTTCGCTGT GAGACAGTCC GAAAGCCTGC TGTCATTGAT 
GCCTATGTGC GCATACGGAC CACAAAAGAT GAGGAATTCA TTCGAAAGTT TGCCCTTTTT 
GATGAACAGC ATCGAGAAGA GATGCGAAAG GAACGGCGGA GGATTCAAGA GCAACTTAGG 
CGGCTCAAGC GGAATCAGGA AAAGGAGAAG CTTAAGGGTC CCCCTGAGAA GAAGCCTAAA 
AAAATGAAGG AGCGTCCTGA TCTAAAACTG AAATGTGGAG CTTGTGGTGC CATTGGGCAC 
ATGAGGACAA ACAAGTTCTG CCCCCTCTAC TATCAAACAA ATGCTCCACC TTCCAACCCT 
GTTGCCATGA CAGAGGAGCA GGAGGAGGAG TTGGAAAAGA CAGTCATTCA TAATGATAAT 
GAAGAACTTA TCAAGGTTGA AGGGACCAAG ATCGTCCTGG GAAAACAGCT AATTGAGAGT 
GCAGATGAGG TTCGAAGAAA ATCTCTGGTT CTCAAGTTCC CTAAACAGCA ACTTCCTCCC 
AAGAAGAAAC GGCGAGTTGG AACCACTGTT CACTGTGACT ACTTAAATAG ACCTCATAAA 
TCCATCCACC GGCGCCGGAC AGACCCAATG GTGACATTGT CCTCCATCTT GGAGTCTATC 
ATCAATGACA TGAGAGATCT TCCAAATACC TACCCTTTCC ACACTCCAGT CAATGCAAAG 
GTTGTAAAGG ACTACTACAA AATCATCACT CGACCAATGG ACTTACAAAC ACTCCGTGAA 
AATGTGCGCA AACGCCTTTA CCCATCTCGG GAAGAGTTCA GAGAACATTT GGAGCTCATT 
GTGAAAAATA GTGCAACCTA TAATGGTCCG AAGCACTCAT TGACCCAGAT CTCTCAATCT 
ATGCTGGATC TCTGTGATGA AAAACTCAAA GAGAAAGAAG ATAAATTGGC TCGTTTAGAG 
AAAGCTATCA ACCCCTTGCT GGATGATGAT GACCAAGTAG CATTTTCTTT CATTCTGGAC 
AACATTGTCA CCCAGAAAAT GATGGCAGTT CCAGATTCTT GGCCATTTCA TCACCCAGTT 
AATAAGAAGT TTGTTCCAGA TTATTACAAA GTGATTATCA GTCCAATGGA TTTAGAGACT 
ATACGTAAGA ATATCTCCAA GCACAAGTAC CAGAGTCGGG AGAGCTTTCT GGATGATGTC 
AACCTTATTC TGGCCAACAG TGTTAAGTAT AATGGGCCTG AAAGTCAGTA TACTAAGACT 
GCTCAGGAGA TTGTAAATGT CTGTTACCAG ACATTGACTG AGTATGATGA ACATTTGACT 
CAACTTGAGA AGGATATTTG TACAGCTAAG GAAGCGGCTT TGGAGGAAGC AGAATTAGAA 
AGCTTGGACC CAATGACCCC AGGGCCTTAT ACACCTCAGG CTAAGCCTCC TGATTTGTAT 
GACACCAACA CATCCCTCAG TATGTCCCGA GATGCCTCTG TATTTCAAGA TGAGAGCAAT 
ATGTCTGTCC TGGATATTCC CACTGCCACT CCAGAAAAGC AGGTGACACA GATGCGCCAG 
GGCCGAGGTA GGCTGGGCGA GGAAGACTCT GATGTAGATA TTGAAGGGTA TGATGATGAG 
GAGGAGGATG GGAAACCTAA GACTCCAGCC CCAGAAGGTG AAGATGCAGA TGGTGATCTT 
GCAGATGAAG AGGAAGGAAG TATGCAACAG CCTCAAGCCA GTGTCCTGTA TGAGGATTTG 
CTTATGTCTG AAGGAGAAGA TGATGAAGAA GATGCTGGGA GTGATGAAGA AGGAGACAAT 
CCTTTCTCTG CTATCCAACT GAGTGAAAGT GGAAGTGACT CTGATGTTGG ATCTAGTGGG 
ATAAGACCCA AACAGCCCCG CATGCTTCAG GAAAACACAA GGATGGGCAT GGAAAATGAA 
GAAAGCATGA TGTCCTATGA GGGAGATGGT GGGGAGGCTT CTCATGGTTT GGAGGATAGC 
AATATGAGTT ATGGGAGCTA TGAGGAGCCT GATCCCAAGT CGAACACCCA AGATACAAGC 
TTCAGCAGCA TCGGTGGGTA TGAGGTATCA GAGGAAGAAG AAGATGAGGA GGAAGAGCAG 
CGCTCTGGGC CAAGTGTACT AAGCCAGGTC CACCTGTCAG AGGATGAGGA GGACAGTGAG 
GATTTCCACT CCATTGCTGG GGACAGTGAT TTGGACTCTG ATGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAF1 ( XM_014111608.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013967083.1 Canis lupus familiaris TATA-box binding protein associated factor 1 (TAF1), transcript variant X5, mRNA.

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CloneID	OCe45585
Clone ID Related Accession (Same CDS sequence)	XM_014111608.1 , XM_014111608.2
Accession Version	XM_014111608.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	transcription initiation factor TFIID subunit 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014111608.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACCTG GCTGGGATTT GCTGCTGCCT GTAGCCGCCG GAGACACTAC CAGCGCCATC 
ATGTCAGACA CGGACAGCGA TGAAGATTCT GCTGGAGGCG GCCCATTTTC TCTAACCGGT 
TTCCTTTTTG GCAACATCAA TGGAGCCGGG CAGCTGGAGG GGGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGTC TGGGCAGCCT GATCACTGAA 
CTCACGGCAA ACGAAGAATT GGCTGGGACC GATGGTGCCC TGGTAAATGA TGAAGGATGG 
ATCAGGAGTA CAGAAGATGC TGTGGACTAT TCAGACATCA CTGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ATCAGCAGAC AATGGGGAGC TTGCAGCCCC TTTGCCACTC AGATTATGAT 
GAAGATGACT ATGATGCCGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAATGAA GAAAGATAAG GACCAGGATG GTCTTACCGG TGTGTCTGAG 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCATCACAG 
GCAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA ACACGATGCC 
ACCAAGCTGT TGCCAAGTGT CACAGAACTT TTCCCGGAAT TTCGGCCTGG GAAGGTATTA 
CGCTTCCTTC GTCTTTTCGG ACCAGGGAAG AATGTCCCAT CTGTTTGGCG GAGTGCTCGG 
AGAAAGAGGA AGAAGAAGCA CCGTGAGCTG ATACAGGAAG AACAGATCCA GGAGATTACA 
ATGATGGCTC CTGTTGAATC AAAGTTTTCC CAGTCAACTG GAGATATAGA TAAAGTGACA 
GATACCAAAC CAAGAGTGGC TGAGTGGCGT TATGGGCCTG CTCGACTGTG GTATGATATG 
CTGGGTGTCC CTGAAGATGG CAGTGGGTTT GATTATGGCT TCAAACTGAG AAAAATGGAC 
CATGAGCCTG TGATAAAATG TAGAATGATG GAGGACTTGA GGAAATTTGA GGAAAACAAT 
GGCTCTGACC TACTGGCTGA TGAAAACTTC CTGATGGTGA CACAGCTGCA CTGGGAGGAT 
GATATCATCT GGGATGGGGA GGATGTCAAG CACAAAGGAA CAAAGCCTCA GCGTGCAAGC 
CTGGCAGGCT GGCTTCCTTC CAGCATGACT AGGAATGCCA TGGCCTACAA TGTTCAGCAA 
GGTTTTGCAG CCACCCTGGA TGATGACAAA CCTTGGTACT CCATCTTTCC CATTGACAAC 
GAGGAGCTGG TATATGGACG CTGGGAGGAC AATATCATTT GGGATGCTCA GGCCATGCCC 
AGGCTGTTGG AGCCTCCTGT TTTGACACTT GATCCCAATG ATGAGAACCT TATTTTGGAA 
ATTCCTGATG AAAAGGAAGA GGCCACTTCT AACTCCCCCT CCAAGGAAAA TAAGAAAGAA 
TCATCTCTGA AGAAGAGCCG AATTCTCTTA GGGAAAACAG GGGTCATCAA GGAGGAACCA 
CAGCAGAACA TGTCTCAGCC AGAAGTGAAA GATCCATGGA ATCTCTCCAA TGATGAATAT 
TACTACCCCA AGCAACAGGG TCTTCGAGGC ACCTTTGGAG GAAATATTAT CCAGCACTCA 
ATTCCTGCAG TGGAATTACG ACAGCCCTTC TTTCCTACCC ATATGGGGCC CATCAAACTT 
CGGCAGTTCC ATCGTCCACC CCTGAAGAAA TATTCCTTCG GGGCACTCTC TCAGCCAGGT 
CCCCACTCAG TCCAGCCCTT GCTGAAGCAC ATCAAAAAGA AGGCTAAGAT GAGAGAACAA 
GAGAGACAAG CTTCTGGTGG TGGAGAGATG TTTTTTATGC GCACACCTCA GGACCTCACA 
GGCAAAGATG GAGATCTTAT TCTTGCAGAA TACAGTGAGG AAAATGGACC ATTAATGATG 
CAGGTTGGCA TGGCAACCAA GATAAAAAAC TATTATAAGC GGAAACCTGG AAAAGATCCT 
GGGGCCCCAG ATTGTAAATA TGGAGAAACT GTTTACTGTC ATACATCTCC TTTCCTAGGC 
TCTCTTCATC CTGGTCAATT ACTGCAGGCA TTTGAGAACA ATCTCTTTCG TGCCCCAATT 
TATCTTCATA AGATGCCAGA AACTGACTTC CTTATTATTC GGACAAGACA AGGTTACTAC 
ATTCGGGAGT TAGTGGATAT TTTTGTGGTC GGCCAGCAAT GCCCCTTGTT TGAAGTTCCT 
GGGCCCAACT CCAAAAGAGC TAATACACAT ATTCGAGACT TTCTCCAGGT TTTTATTTAC 
CGCCTCTTCT GGAAGAGTAA AGATCGGCCA AGGCGGATCC GAATGGAAGA TATAAAAAAA 
GCCTTTCCTT CTCACTCAGA AAGTAGCATC CGCAAGAGGC TAAAGCTCTG CGCTGACTTC 
AAACGCACAG GGATGGACTC AAACTGGTGG GTGCTAAAGT CAGATTTTCG TTTACCAACT 
GAAGAGGAGA TCAGAGCTAT GGTGTCTCCA GAGCAGTGCT GTGCTTATTA TAGCATGATA 
GCTGCAGAGC AGCGACTAAA GGATGCTGGC TATGGTGAGA AGTCCTTTTT TGCTCCAGAA 
GAAGAAAATG AGGAAGATTT CCAGATGAAG ATTGATGATG AAGTTCGCAC TGCTCCTTGG 
AATACCACAA GGGCTTTCAT TGCTGCCATG AAGGGCAAGT GTCTCTTGGA GGTGACTGGG 
GTGGCAGATC CCACAGGGTG TGGTGAAGGA TTCTCCTATG TCAAGATTCC AAACAAACCA 
ACACAGCAGA AGGATGATAA GGAGCCTCAG CCAGTGAAGA AGACAGTGAC AGGAACAGAT 
GCAGATCTCC GTCGCCTCTC ACTGAAAAAT GCCAAGCAGC TCCTACGTAA ATTTGGTGTG 
CCTGAGGAAG AGATTAAAAA GCTGTCCCGC TGGGAAGTGA TTGATGTGGT ACGCACAATG 
TCAACAGAGC AAGCCCGCTC TGGAGAGGGG CCCATGAGTA AATTTGCCCG TGGATCAAGG 
TTTTCTGTGG CTGAGCATCA AGAGCGTTAC AAAGAGGAAT GTCAGCGCAT CTTTGATCTA 
CAGAACAAGG TTTTGTCATC AACTGAAATA TTGTCAACTG ACACAGACAG CAGCTCAGCC 
GAAGACAGTG ACTTTGAAGA AATGGGAAAG AACATTGAGA ACATGTTGCA GAATAAGAAA 
ACCAGCTCTC AGCTGTCACG TGAACGAGAG GAGCAGGAGC GGAAGGAACT GCAGCGGATG 
CTACTGGCAG CAGGTTCTGC AGCATCAGGA AACAATCACA GAGATGATGA TACAGCTTCT 
GTGACCAGCC TTAACTCTTC TGCCACTGGC CGTTGTCTCA AAATTTATCG CACATTTCGA 
GATGAAGAAG GGAAAGAGTA TGTTCGCTGT GAGACAGTCC GAAAGCCTGC TGTCATTGAT 
GCCTATGTGC GCATACGGAC CACAAAAGAT GAGGAATTCA TTCGAAAGTT TGCCCTTTTT 
GATGAACAGC ATCGAGAAGA GATGCGAAAG GAACGGCGGA GGATTCAAGA GCAACTTAGG 
CGGCTCAAGC GGAATCAGGA AAAGGAGAAG CTTAAGGGTC CCCCTGAGAA GAAGCCTAAA 
AAAATGAAGG AGCGTCCTGA TCTAAAACTG AAATGTGGAG CTTGTGGTGC CATTGGGCAC 
ATGAGGACAA ACAAGTTCTG CCCCCTCTAC TATCAAACAA ATGCTCCACC TTCCAACCCT 
GTTGCCATGA CAGAGGAGCA GGAGGAGGAG TTGGAAAAGA CAGTCATTCA TAATGATAAT 
GAAGAACTTA TCAAGGTTGA AGGGACCAAG ATCGTCCTGG GAAAACAGCT AATTGAGAGT 
GCAGATGAGG TTCGAAGAAA ATCTCTGGTT CTCAAGTTCC CTAAACAGCA ACTTCCTCCC 
AAGAAGAAAC GGCGAGTTGG AACCACTGTT CACTGTGACT ACTTAAATAG ACCTCATAAA 
TCCATCCACC GGCGCCGGAC AGACCCAATG GTGACATTGT CCTCCATCTT GGAGTCTATC 
ATCAATGACA TGAGAGATCT TCCAAATACC TACCCTTTCC ACACTCCAGT CAATGCAAAG 
GTTGTAAAGG ACTACTACAA AATCATCACT CGACCAATGG ACTTACAAAC ACTCCGTGAA 
AATGTGCGCA AACGCCTTTA CCCATCTCGG GAAGAGTTCA GAGAACATTT GGAGCTCATT 
GTGAAAAATA GTGCAACCTA TAATGGTCCG AAGCACTCAT TGACCCAGAT CTCTCAATCT 
ATGCTGGATC TCTGTGATGA AAAACTCAAA GAGAAAGAAG ATAAATTGGC TCGTTTAGAG 
AAAGCTATCA ACCCCTTGCT GGATGATGAT GACCAAGTAG CATTTTCTTT CATTCTGGAC 
AACATTGTCA CCCAGAAAAT GATGGCAGTT CCAGATTCTT GGCCATTTCA TCACCCAGTT 
AATAAGAAGT TTGTTCCAGA TTATTACAAA GTGATTATCA GTCCAATGGA TTTAGAGACT 
ATACGTAAGA ATATCTCCAA GCACAAGTAC CAGAGTCGGG AGAGCTTTCT GGATGATGTC 
AACCTTATTC TGGCCAACAG TGTTAAGTAT AATGGGCCTG AAAGTCAGTA TACTAAGACT 
GCTCAGGAGA TTGTAAATGT CTGTTACCAG ACATTGACTG AGTATGATGA ACATTTGACT 
CAACTTGAGA AGGATATTTG TACAGCTAAG GAAGCGGCTT TGGAGGAAGC AGAATTAGAA 
AGCTTGGACC CAATGACCCC AGGGCCTTAT ACACCTCAGG CTAAGCCTCC TGATTTGTAT 
GACACCAACA CATCCCTCAG TATGTCCCGA GATGCCTCTG TATTTCAAGA TGAGAGCAAT 
ATGTCTGTCC TGGATATTCC CACTGCCACT CCAGAAAAGC AGGTGACACA GATGCGCCAG 
GGCCGAGGTA GGCTGGGCGA GGAAGACTCT GATGTAGATA TTGAAGGGTA TGATGATGAG 
GAGGAGGATG GGAAACCTAA GACTCCAGCC CCAGAAGGTG AAGATGCAGA TGGTGATCTT 
GCAGATGAAG AGGAAGGAAG TATGCAACAG CCTCAAGCCA GTGTCCTGTA TGAGGATTTG 
CTTATGTCTG AAGGAGAAGA TGATGAAGAA GATGCTGGGA GTGATGAAGA AGGAGACAAT 
CCTTTCTCTG CTATCCAACT GAGTGAAAGT GGAAGTGACT CTGATGTTGG ATCTAGTGGG 
ATAAGACCCA AACAGCCCCG CATGCTTCAG GAAAACACAA GGATGGGCAT GGAAAATGAA 
GAAAGCATGA TGTCCTATGA GGGAGATGGT GGGGAGGCTT CTCATGGTTT GGAGGATAGC 
AATATGAGTT ATGGGAGCTA TGAGGAGCCT GATCCCAAGT CGAACACCCA AGATACAAGC 
TTCAGCAGCA TCGGTGGGTA TGAGGTATCA GAGGAAGAAG AAGATGAGGA GGAAGAGCAG 
CGCTCTGGGC CAAGTGTACT AAGCCAGGTC CACCTGTCAG AGGATGAGGA GGACAGTGAG 
GATTTCCACT CCATTGCTGG GGACAGTGAT TTGGACTCTG ATGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967083.1
CDS	104..5791
Translation	MGPGWDLLLPVAAGDTTSAIMSDTDSDEDSAGGGPFSLTGFLFGNINGAGQLEGESVLDDECKKHLAGLGALGLGSLITELTANEELAGTDGALVNDEGWIRSTEDAVDYSDITEVAEDESRRYQQTMGSLQPLCHSDYDEDDYDADCEDIDCKLMPPPPPPPGPMKKDKDQDGLTGVSEDGEGIILPSIIAPSSLASEKVDFSSSSDSESEMGPQEASQAESEDGKLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSARRKRKKKHRELIQEEQIQEITMMAPVESKFSQSTGDIDKVTDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKLRKMDHEPVIKCRMMEDLRKFEENNGSDLLADENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDKPWYSIFPIDNEELVYGRWEDNIIWDAQAMPRLLEPPVLTLDPNDENLILEIPDEKEEATSNSPSKENKKESSLKKSRILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYYIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYYSMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEILSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMLLAAGSAASGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKPAVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIISPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNGPESQYTKTAQEIVNVCYQTLTEYDEHLTQLEKDICTAKEAALEEAELESLDPMTPGPYTPQAKPPDLYDTNTSLSMSRDASVFQDESNMSVLDIPTATPEKQVTQMRQGRGRLGEEDSDVDIEGYDDEEEDGKPKTPAPEGEDADGDLADEEEGSMQQPQASVLYEDLLMSEGEDDEEDAGSDEEGDNPFSAIQLSESGSDSDVGSSGIRPKQPRMLQENTRMGMENEESMMSYEGDGGEASHGLEDSNMSYGSYEEPDPKSNTQDTSFSSIGGYEVSEEEEDEEEEQRSGPSVLSQVHLSEDEEDSEDFHSIAGDSDLDSDE

Target ORF information:

RefSeq Version	XM_014111608.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris TATA-box binding protein associated factor 1 (TAF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111608.2

ORF Insert Sequence:

ATGGGACCTG GCTGGGATTT GCTGCTGCCT GTAGCCGCCG GAGACACTAC CAGCGCCATC 
ATGTCAGACA CGGACAGCGA TGAAGATTCT GCTGGAGGCG GCCCATTTTC TCTAACCGGT 
TTCCTTTTTG GCAACATCAA TGGAGCCGGG CAGCTGGAGG GGGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGTC TGGGCAGCCT GATCACTGAA 
CTCACGGCAA ACGAAGAATT GGCTGGGACC GATGGTGCCC TGGTAAATGA TGAAGGATGG 
ATCAGGAGTA CAGAAGATGC TGTGGACTAT TCAGACATCA CTGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ATCAGCAGAC AATGGGGAGC TTGCAGCCCC TTTGCCACTC AGATTATGAT 
GAAGATGACT ATGATGCCGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAATGAA GAAAGATAAG GACCAGGATG GTCTTACCGG TGTGTCTGAG 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCATCACAG 
GCAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA ACACGATGCC 
ACCAAGCTGT TGCCAAGTGT CACAGAACTT TTCCCGGAAT TTCGGCCTGG GAAGGTATTA 
CGCTTCCTTC GTCTTTTCGG ACCAGGGAAG AATGTCCCAT CTGTTTGGCG GAGTGCTCGG 
AGAAAGAGGA AGAAGAAGCA CCGTGAGCTG ATACAGGAAG AACAGATCCA GGAGATTACA 
ATGATGGCTC CTGTTGAATC AAAGTTTTCC CAGTCAACTG GAGATATAGA TAAAGTGACA 
GATACCAAAC CAAGAGTGGC TGAGTGGCGT TATGGGCCTG CTCGACTGTG GTATGATATG 
CTGGGTGTCC CTGAAGATGG CAGTGGGTTT GATTATGGCT TCAAACTGAG AAAAATGGAC 
CATGAGCCTG TGATAAAATG TAGAATGATG GAGGACTTGA GGAAATTTGA GGAAAACAAT 
GGCTCTGACC TACTGGCTGA TGAAAACTTC CTGATGGTGA CACAGCTGCA CTGGGAGGAT 
GATATCATCT GGGATGGGGA GGATGTCAAG CACAAAGGAA CAAAGCCTCA GCGTGCAAGC 
CTGGCAGGCT GGCTTCCTTC CAGCATGACT AGGAATGCCA TGGCCTACAA TGTTCAGCAA 
GGTTTTGCAG CCACCCTGGA TGATGACAAA CCTTGGTACT CCATCTTTCC CATTGACAAC 
GAGGAGCTGG TATATGGACG CTGGGAGGAC AATATCATTT GGGATGCTCA GGCCATGCCC 
AGGCTGTTGG AGCCTCCTGT TTTGACACTT GATCCCAATG ATGAGAACCT TATTTTGGAA 
ATTCCTGATG AAAAGGAAGA GGCCACTTCT AACTCCCCCT CCAAGGAAAA TAAGAAAGAA 
TCATCTCTGA AGAAGAGCCG AATTCTCTTA GGGAAAACAG GGGTCATCAA GGAGGAACCA 
CAGCAGAACA TGTCTCAGCC AGAAGTGAAA GATCCATGGA ATCTCTCCAA TGATGAATAT 
TACTACCCCA AGCAACAGGG TCTTCGAGGC ACCTTTGGAG GAAATATTAT CCAGCACTCA 
ATTCCTGCAG TGGAATTACG ACAGCCCTTC TTTCCTACCC ATATGGGGCC CATCAAACTT 
CGGCAGTTCC ATCGTCCACC CCTGAAGAAA TATTCCTTCG GGGCACTCTC TCAGCCAGGT 
CCCCACTCAG TCCAGCCCTT GCTGAAGCAC ATCAAAAAGA AGGCTAAGAT GAGAGAACAA 
GAGAGACAAG CTTCTGGTGG TGGAGAGATG TTTTTTATGC GCACACCTCA GGACCTCACA 
GGCAAAGATG GAGATCTTAT TCTTGCAGAA TACAGTGAGG AAAATGGACC ATTAATGATG 
CAGGTTGGCA TGGCAACCAA GATAAAAAAC TATTATAAGC GGAAACCTGG AAAAGATCCT 
GGGGCCCCAG ATTGTAAATA TGGAGAAACT GTTTACTGTC ATACATCTCC TTTCCTAGGC 
TCTCTTCATC CTGGTCAATT ACTGCAGGCA TTTGAGAACA ATCTCTTTCG TGCCCCAATT 
TATCTTCATA AGATGCCAGA AACTGACTTC CTTATTATTC GGACAAGACA AGGTTACTAC 
ATTCGGGAGT TAGTGGATAT TTTTGTGGTC GGCCAGCAAT GCCCCTTGTT TGAAGTTCCT 
GGGCCCAACT CCAAAAGAGC TAATACACAT ATTCGAGACT TTCTCCAGGT TTTTATTTAC 
CGCCTCTTCT GGAAGAGTAA AGATCGGCCA AGGCGGATCC GAATGGAAGA TATAAAAAAA 
GCCTTTCCTT CTCACTCAGA AAGTAGCATC CGCAAGAGGC TAAAGCTCTG CGCTGACTTC 
AAACGCACAG GGATGGACTC AAACTGGTGG GTGCTAAAGT CAGATTTTCG TTTACCAACT 
GAAGAGGAGA TCAGAGCTAT GGTGTCTCCA GAGCAGTGCT GTGCTTATTA TAGCATGATA 
GCTGCAGAGC AGCGACTAAA GGATGCTGGC TATGGTGAGA AGTCCTTTTT TGCTCCAGAA 
GAAGAAAATG AGGAAGATTT CCAGATGAAG ATTGATGATG AAGTTCGCAC TGCTCCTTGG 
AATACCACAA GGGCTTTCAT TGCTGCCATG AAGGGCAAGT GTCTCTTGGA GGTGACTGGG 
GTGGCAGATC CCACAGGGTG TGGTGAAGGA TTCTCCTATG TCAAGATTCC AAACAAACCA 
ACACAGCAGA AGGATGATAA GGAGCCTCAG CCAGTGAAGA AGACAGTGAC AGGAACAGAT 
GCAGATCTCC GTCGCCTCTC ACTGAAAAAT GCCAAGCAGC TCCTACGTAA ATTTGGTGTG 
CCTGAGGAAG AGATTAAAAA GCTGTCCCGC TGGGAAGTGA TTGATGTGGT ACGCACAATG 
TCAACAGAGC AAGCCCGCTC TGGAGAGGGG CCCATGAGTA AATTTGCCCG TGGATCAAGG 
TTTTCTGTGG CTGAGCATCA AGAGCGTTAC AAAGAGGAAT GTCAGCGCAT CTTTGATCTA 
CAGAACAAGG TTTTGTCATC AACTGAAATA TTGTCAACTG ACACAGACAG CAGCTCAGCC 
GAAGACAGTG ACTTTGAAGA AATGGGAAAG AACATTGAGA ACATGTTGCA GAATAAGAAA 
ACCAGCTCTC AGCTGTCACG TGAACGAGAG GAGCAGGAGC GGAAGGAACT GCAGCGGATG 
CTACTGGCAG CAGGTTCTGC AGCATCAGGA AACAATCACA GAGATGATGA TACAGCTTCT 
GTGACCAGCC TTAACTCTTC TGCCACTGGC CGTTGTCTCA AAATTTATCG CACATTTCGA 
GATGAAGAAG GGAAAGAGTA TGTTCGCTGT GAGACAGTCC GAAAGCCTGC TGTCATTGAT 
GCCTATGTGC GCATACGGAC CACAAAAGAT GAGGAATTCA TTCGAAAGTT TGCCCTTTTT 
GATGAACAGC ATCGAGAAGA GATGCGAAAG GAACGGCGGA GGATTCAAGA GCAACTTAGG 
CGGCTCAAGC GGAATCAGGA AAAGGAGAAG CTTAAGGGTC CCCCTGAGAA GAAGCCTAAA 
AAAATGAAGG AGCGTCCTGA TCTAAAACTG AAATGTGGAG CTTGTGGTGC CATTGGGCAC 
ATGAGGACAA ACAAGTTCTG CCCCCTCTAC TATCAAACAA ATGCTCCACC TTCCAACCCT 
GTTGCCATGA CAGAGGAGCA GGAGGAGGAG TTGGAAAAGA CAGTCATTCA TAATGATAAT 
GAAGAACTTA TCAAGGTTGA AGGGACCAAG ATCGTCCTGG GAAAACAGCT AATTGAGAGT 
GCAGATGAGG TTCGAAGAAA ATCTCTGGTT CTCAAGTTCC CTAAACAGCA ACTTCCTCCC 
AAGAAGAAAC GGCGAGTTGG AACCACTGTT CACTGTGACT ACTTAAATAG ACCTCATAAA 
TCCATCCACC GGCGCCGGAC AGACCCAATG GTGACATTGT CCTCCATCTT GGAGTCTATC 
ATCAATGACA TGAGAGATCT TCCAAATACC TACCCTTTCC ACACTCCAGT CAATGCAAAG 
GTTGTAAAGG ACTACTACAA AATCATCACT CGACCAATGG ACTTACAAAC ACTCCGTGAA 
AATGTGCGCA AACGCCTTTA CCCATCTCGG GAAGAGTTCA GAGAACATTT GGAGCTCATT 
GTGAAAAATA GTGCAACCTA TAATGGTCCG AAGCACTCAT TGACCCAGAT CTCTCAATCT 
ATGCTGGATC TCTGTGATGA AAAACTCAAA GAGAAAGAAG ATAAATTGGC TCGTTTAGAG 
AAAGCTATCA ACCCCTTGCT GGATGATGAT GACCAAGTAG CATTTTCTTT CATTCTGGAC 
AACATTGTCA CCCAGAAAAT GATGGCAGTT CCAGATTCTT GGCCATTTCA TCACCCAGTT 
AATAAGAAGT TTGTTCCAGA TTATTACAAA GTGATTATCA GTCCAATGGA TTTAGAGACT 
ATACGTAAGA ATATCTCCAA GCACAAGTAC CAGAGTCGGG AGAGCTTTCT GGATGATGTC 
AACCTTATTC TGGCCAACAG TGTTAAGTAT AATGGGCCTG AAAGTCAGTA TACTAAGACT 
GCTCAGGAGA TTGTAAATGT CTGTTACCAG ACATTGACTG AGTATGATGA ACATTTGACT 
CAACTTGAGA AGGATATTTG TACAGCTAAG GAAGCGGCTT TGGAGGAAGC AGAATTAGAA 
AGCTTGGACC CAATGACCCC AGGGCCTTAT ACACCTCAGG CTAAGCCTCC TGATTTGTAT 
GACACCAACA CATCCCTCAG TATGTCCCGA GATGCCTCTG TATTTCAAGA TGAGAGCAAT 
ATGTCTGTCC TGGATATTCC CACTGCCACT CCAGAAAAGC AGGTGACACA GATGCGCCAG 
GGCCGAGGTA GGCTGGGCGA GGAAGACTCT GATGTAGATA TTGAAGGGTA TGATGATGAG 
GAGGAGGATG GGAAACCTAA GACTCCAGCC CCAGAAGGTG AAGATGCAGA TGGTGATCTT 
GCAGATGAAG AGGAAGGAAG TATGCAACAG CCTCAAGCCA GTGTCCTGTA TGAGGATTTG 
CTTATGTCTG AAGGAGAAGA TGATGAAGAA GATGCTGGGA GTGATGAAGA AGGAGACAAT 
CCTTTCTCTG CTATCCAACT GAGTGAAAGT GGAAGTGACT CTGATGTTGG ATCTAGTGGG 
ATAAGACCCA AACAGCCCCG CATGCTTCAG GAAAACACAA GGATGGGCAT GGAAAATGAA 
GAAAGCATGA TGTCCTATGA GGGAGATGGT GGGGAGGCTT CTCATGGTTT GGAGGATAGC 
AATATGAGTT ATGGGAGCTA TGAGGAGCCT GATCCCAAGT CGAACACCCA AGATACAAGC 
TTCAGCAGCA TCGGTGGGTA TGAGGTATCA GAGGAAGAAG AAGATGAGGA GGAAGAGCAG 
CGCTCTGGGC CAAGTGTACT AAGCCAGGTC CACCTGTCAG AGGATGAGGA GGACAGTGAG 
GATTTCCACT CCATTGCTGG GGACAGTGAT TTGGACTCTG ATGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAF1 ( XM_005641473.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005641530.1 Canis lupus familiaris TATA-box binding protein associated factor 1 (TAF1), transcript variant X2, mRNA.

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CloneID	OCe32527
Clone ID Related Accession (Same CDS sequence)	XM_005641473.3 , XM_005641473.2
Accession Version	XM_005641473.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5784bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	transcription initiation factor TFIID subunit 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005641473.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACCTG GCTGGGATTT GCTGCTGCCT GTAGCCGCCG GAGACACTAC CAGCGCCATC 
ATGTCAGACA CGGACAGCGA TGAAGATTCT GCTGGAGGCG GCCCATTTTC TCTAACCGGT 
TTCCTTTTTG GCAACATCAA TGGAGCCGGG CAGCTGGAGG GGGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGTC TGGGCAGCCT GATCACTGAA 
CTCACGGCAA ACGAAGAATT GGCTGGGACC GATGGTGCCC TGGTAAATGA TGAAGGATGG 
ATCAGGAGTA CAGAAGATGC TGTGGACTAT TCAGACATCA CTGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ATCAGCAGAC AATGGGGAGC TTGCAGCCCC TTTGCCACTC AGATTATGAT 
GAAGATGACT ATGATGCCGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAATGAA GAAAGATAAG GACCAGGATG GTCTTACCGG TGTGTCTGAG 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCATCACAG 
GCAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA ACACGATGCC 
ACCAAGCTGT TGCCAAGTGT CACAGAACTT TTCCCGGAAT TTCGGCCTGG GAAGGTATTA 
CGCTTCCTTC GTCTTTTCGG ACCAGGGAAG AATGTCCCAT CTGTTTGGCG GAGTGCTCGG 
AGAAAGAGGA AGAAGAAGCA CCGTGAGCTG ATACAGGAAG AACAGATCCA GGAGGTGGAG 
TGCTCAGTAG AGTCCGAAGT CAACAAGAAG TCTTTATGGA ATTATGACTA TGCTCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATTACAA TGATGGCTCC TGTTGAATCA 
AAGTTTTCCC AGTCAACTGG AGATATAGAT AAAGTGACAG ATACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ATTATGGCTT CAAACTGAGA AAAATGGACC ATGAGCCTGT GATAAAATGT 
AGAATGATGG AGGACTTGAG GAAATTTGAG GAAAACAATG GCTCTGACCT ACTGGCTGAT 
GAAAACTTCC TGATGGTGAC ACAGCTGCAC TGGGAGGATG ATATCATCTG GGATGGGGAG 
GATGTCAAGC ACAAAGGAAC AAAGCCTCAG CGTGCAAGCC TGGCAGGCTG GCTTCCTTCC 
AGCATGACTA GGAATGCCAT GGCCTACAAT GTTCAGCAAG GTTTTGCAGC CACCCTGGAT 
GATGACAAAC CTTGGTACTC CATCTTTCCC ATTGACAACG AGGAGCTGGT ATATGGACGC 
TGGGAGGACA ATATCATTTG GGATGCTCAG GCCATGCCCA GGCTGTTGGA GCCTCCTGTT 
TTGACACTTG ATCCCAATGA TGAGAACCTT ATTTTGGAAA TTCCTGATGA AAAGGAAGAG 
GCCACTTCTA ACTCCCCCTC CAAGGAAAAT AAGAAAGAAT CATCTCTGAA GAAGAGCCGA 
ATTCTCTTAG GGAAAACAGG GGTCATCAAG GAGGAACCAC AGCAGAACAT GTCTCAGCCA 
GAAGTGAAAG ATCCATGGAA TCTCTCCAAT GATGAATATT ACTACCCCAA GCAACAGGGT 
CTTCGAGGCA CCTTTGGAGG AAATATTATC CAGCACTCAA TTCCTGCAGT GGAATTACGA 
CAGCCCTTCT TTCCTACCCA TATGGGGCCC ATCAAACTTC GGCAGTTCCA TCGTCCACCC 
CTGAAGAAAT ATTCCTTCGG GGCACTCTCT CAGCCAGGTC CCCACTCAGT CCAGCCCTTG 
CTGAAGCACA TCAAAAAGAA GGCTAAGATG AGAGAACAAG AGAGACAAGC TTCTGGTGGT 
GGAGAGATGT TTTTTATGCG CACACCTCAG GACCTCACAG GCAAAGATGG AGATCTTATT 
CTTGCAGAAT ACAGTGAGGA AAATGGACCA TTAATGATGC AGGTTGGCAT GGCAACCAAG 
ATAAAAAACT ATTATAAGCG GAAACCTGGA AAAGATCCTG GGGCCCCAGA TTGTAAATAT 
GGAGAAACTG TTTACTGTCA TACATCTCCT TTCCTAGGCT CTCTTCATCC TGGTCAATTA 
CTGCAGGCAT TTGAGAACAA TCTCTTTCGT GCCCCAATTT ATCTTCATAA GATGCCAGAA 
ACTGACTTCC TTATTATTCG GACAAGACAA GGTTACTACA TTCGGGAGTT AGTGGATATT 
TTTGTGGTCG GCCAGCAATG CCCCTTGTTT GAAGTTCCTG GGCCCAACTC CAAAAGAGCT 
AATACACATA TTCGAGACTT TCTCCAGGTT TTTATTTACC GCCTCTTCTG GAAGAGTAAA 
GATCGGCCAA GGCGGATCCG AATGGAAGAT ATAAAAAAAG CCTTTCCTTC TCACTCAGAA 
AGTAGCATCC GCAAGAGGCT AAAGCTCTGC GCTGACTTCA AACGCACAGG GATGGACTCA 
AACTGGTGGG TGCTAAAGTC AGATTTTCGT TTACCAACTG AAGAGGAGAT CAGAGCTATG 
GTGTCTCCAG AGCAGTGCTG TGCTTATTAT AGCATGATAG CTGCAGAGCA GCGACTAAAG 
GATGCTGGCT ATGGTGAGAA GTCCTTTTTT GCTCCAGAAG AAGAAAATGA GGAAGATTTC 
CAGATGAAGA TTGATGATGA AGTTCGCACT GCTCCTTGGA ATACCACAAG GGCTTTCATT 
GCTGCCATGA AGGGCAAGTG TCTCTTGGAG GTGACTGGGG TGGCAGATCC CACAGGGTGT 
GGTGAAGGAT TCTCCTATGT CAAGATTCCA AACAAACCAA CACAGCAGAA GGATGATAAG 
GAGCCTCAGC CAGTGAAGAA GACAGTGACA GGAACAGATG CAGATCTCCG TCGCCTCTCA 
CTGAAAAATG CCAAGCAGCT CCTACGTAAA TTTGGTGTGC CTGAGGAAGA GATTAAAAAG 
CTGTCCCGCT GGGAAGTGAT TGATGTGGTA CGCACAATGT CAACAGAGCA AGCCCGCTCT 
GGAGAGGGGC CCATGAGTAA ATTTGCCCGT GGATCAAGGT TTTCTGTGGC TGAGCATCAA 
GAGCGTTACA AAGAGGAATG TCAGCGCATC TTTGATCTAC AGAACAAGGT TTTGTCATCA 
ACTGAAATAT TGTCAACTGA CACAGACAGC AGCTCAGCCG AAGACAGTGA CTTTGAAGAA 
ATGGGAAAGA ACATTGAGAA CATGTTGCAG AATAAGAAAA CCAGCTCTCA GCTGTCACGT 
GAACGAGAGG AGCAGGAGCG GAAGGAACTG CAGCGGATGC TACTGGCAGG TTCTGCAGCA 
TCAGGAAACA ATCACAGAGA TGATGATACA GCTTCTGTGA CCAGCCTTAA CTCTTCTGCC 
ACTGGCCGTT GTCTCAAAAT TTATCGCACA TTTCGAGATG AAGAAGGGAA AGAGTATGTT 
CGCTGTGAGA CAGTCCGAAA GCCTGCTGTC ATTGATGCCT ATGTGCGCAT ACGGACCACA 
AAAGATGAGG AATTCATTCG AAAGTTTGCC CTTTTTGATG AACAGCATCG AGAAGAGATG 
CGAAAGGAAC GGCGGAGGAT TCAAGAGCAA CTTAGGCGGC TCAAGCGGAA TCAGGAAAAG 
GAGAAGCTTA AGGGTCCCCC TGAGAAGAAG CCTAAAAAAA TGAAGGAGCG TCCTGATCTA 
AAACTGAAAT GTGGAGCTTG TGGTGCCATT GGGCACATGA GGACAAACAA GTTCTGCCCC 
CTCTACTATC AAACAAATGC TCCACCTTCC AACCCTGTTG CCATGACAGA GGAGCAGGAG 
GAGGAGTTGG AAAAGACAGT CATTCATAAT GATAATGAAG AACTTATCAA GGTTGAAGGG 
ACCAAGATCG TCCTGGGAAA ACAGCTAATT GAGAGTGCAG ATGAGGTTCG AAGAAAATCT 
CTGGTTCTCA AGTTCCCTAA ACAGCAACTT CCTCCCAAGA AGAAACGGCG AGTTGGAACC 
ACTGTTCACT GTGACTACTT AAATAGACCT CATAAATCCA TCCACCGGCG CCGGACAGAC 
CCAATGGTGA CATTGTCCTC CATCTTGGAG TCTATCATCA ATGACATGAG AGATCTTCCA 
AATACCTACC CTTTCCACAC TCCAGTCAAT GCAAAGGTTG TAAAGGACTA CTACAAAATC 
ATCACTCGAC CAATGGACTT ACAAACACTC CGTGAAAATG TGCGCAAACG CCTTTACCCA 
TCTCGGGAAG AGTTCAGAGA ACATTTGGAG CTCATTGTGA AAAATAGTGC AACCTATAAT 
GGTCCGAAGC ACTCATTGAC CCAGATCTCT CAATCTATGC TGGATCTCTG TGATGAAAAA 
CTCAAAGAGA AAGAAGATAA ATTGGCTCGT TTAGAGAAAG CTATCAACCC CTTGCTGGAT 
GATGATGACC AAGTAGCATT TTCTTTCATT CTGGACAACA TTGTCACCCA GAAAATGATG 
GCAGTTCCAG ATTCTTGGCC ATTTCATCAC CCAGTTAATA AGAAGTTTGT TCCAGATTAT 
TACAAAGTGA TTATCAGTCC AATGGATTTA GAGACTATAC GTAAGAATAT CTCCAAGCAC 
AAGTACCAGA GTCGGGAGAG CTTTCTGGAT GATGTCAACC TTATTCTGGC CAACAGTGTT 
AAGTATAATG GGCCTGAAAG TCAGTATACT AAGACTGCTC AGGAGATTGT AAATGTCTGT 
TACCAGACAT TGACTGAGTA TGATGAACAT TTGACTCAAC TTGAGAAGGA TATTTGTACA 
GCTAAGGAAG CGGCTTTGGA GGAAGCAGAA TTAGAAAGCT TGGACCCAAT GACCCCAGGG 
CCTTATACAC CTCAGGCTAA GCCTCCTGAT TTGTATGACA CCAACACATC CCTCAGTATG 
TCCCGAGATG CCTCTGTATT TCAAGATGAG AGCAATATGT CTGTCCTGGA TATTCCCACT 
GCCACTCCAG AAAAGCAGGT GACACAGATG CGCCAGGGCC GAGGTAGGCT GGGCGAGGAA 
GACTCTGATG TAGATATTGA AGGGTATGAT GATGAGGAGG AGGATGGGAA ACCTAAGACT 
CCAGCCCCAG AAGGTGAAGA TGCAGATGGT GATCTTGCAG ATGAAGAGGA AGGAAGTATG 
CAACAGCCTC AAGCCAGTGT CCTGTATGAG GATTTGCTTA TGTCTGAAGG AGAAGATGAT 
GAAGAAGATG CTGGGAGTGA TGAAGAAGGA GACAATCCTT TCTCTGCTAT CCAACTGAGT 
GAAAGTGGAA GTGACTCTGA TGTTGGATCT AGTGGGATAA GACCCAAACA GCCCCGCATG 
CTTCAGGAAA ACACAAGGAT GGGCATGGAA AATGAAGAAA GCATGATGTC CTATGAGGGA 
GATGGTGGGG AGGCTTCTCA TGGTTTGGAG GATAGCAATA TGAGTTATGG GAGCTATGAG 
GAGCCTGATC CCAAGTCGAA CACCCAAGAT ACAAGCTTCA GCAGCATCGG TGGGTATGAG 
GTATCAGAGG AAGAAGAAGA TGAGGAGGAA GAGCAGCGCT CTGGGCCAAG TGTACTAAGC 
CAGGTCCACC TGTCAGAGGA TGAGGAGGAC AGTGAGGATT TCCACTCCAT TGCTGGGGAC 
AGTGATTTGG ACTCTGATGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005641530.1
CDS	104..5887
Translation	MGPGWDLLLPVAAGDTTSAIMSDTDSDEDSAGGGPFSLTGFLFGNINGAGQLEGESVLDDECKKHLAGLGALGLGSLITELTANEELAGTDGALVNDEGWIRSTEDAVDYSDITEVAEDESRRYQQTMGSLQPLCHSDYDEDDYDADCEDIDCKLMPPPPPPPGPMKKDKDQDGLTGVSEDGEGIILPSIIAPSSLASEKVDFSSSSDSESEMGPQEASQAESEDGKLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSARRKRKKKHRELIQEEQIQEVECSVESEVNKKSLWNYDYAPPPPPEQCLSDDEITMMAPVESKFSQSTGDIDKVTDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKLRKMDHEPVIKCRMMEDLRKFEENNGSDLLADENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDKPWYSIFPIDNEELVYGRWEDNIIWDAQAMPRLLEPPVLTLDPNDENLILEIPDEKEEATSNSPSKENKKESSLKKSRILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYYIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYYSMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEILSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMLLAGSAASGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKPAVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIISPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNGPESQYTKTAQEIVNVCYQTLTEYDEHLTQLEKDICTAKEAALEEAELESLDPMTPGPYTPQAKPPDLYDTNTSLSMSRDASVFQDESNMSVLDIPTATPEKQVTQMRQGRGRLGEEDSDVDIEGYDDEEEDGKPKTPAPEGEDADGDLADEEEGSMQQPQASVLYEDLLMSEGEDDEEDAGSDEEGDNPFSAIQLSESGSDSDVGSSGIRPKQPRMLQENTRMGMENEESMMSYEGDGGEASHGLEDSNMSYGSYEEPDPKSNTQDTSFSSIGGYEVSEEEEDEEEEQRSGPSVLSQVHLSEDEEDSEDFHSIAGDSDLDSDE

Target ORF information:

RefSeq Version	XM_005641473.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris TATA-box binding protein associated factor 1 (TAF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005641473.3

ORF Insert Sequence:

ATGGGACCTG GCTGGGATTT GCTGCTGCCT GTAGCCGCCG GAGACACTAC CAGCGCCATC 
ATGTCAGACA CGGACAGCGA TGAAGATTCT GCTGGAGGCG GCCCATTTTC TCTAACCGGT 
TTCCTTTTTG GCAACATCAA TGGAGCCGGG CAGCTGGAGG GGGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGTC TGGGCAGCCT GATCACTGAA 
CTCACGGCAA ACGAAGAATT GGCTGGGACC GATGGTGCCC TGGTAAATGA TGAAGGATGG 
ATCAGGAGTA CAGAAGATGC TGTGGACTAT TCAGACATCA CTGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ATCAGCAGAC AATGGGGAGC TTGCAGCCCC TTTGCCACTC AGATTATGAT 
GAAGATGACT ATGATGCCGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAATGAA GAAAGATAAG GACCAGGATG GTCTTACCGG TGTGTCTGAG 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCATCACAG 
GCAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA ACACGATGCC 
ACCAAGCTGT TGCCAAGTGT CACAGAACTT TTCCCGGAAT TTCGGCCTGG GAAGGTATTA 
CGCTTCCTTC GTCTTTTCGG ACCAGGGAAG AATGTCCCAT CTGTTTGGCG GAGTGCTCGG 
AGAAAGAGGA AGAAGAAGCA CCGTGAGCTG ATACAGGAAG AACAGATCCA GGAGGTGGAG 
TGCTCAGTAG AGTCCGAAGT CAACAAGAAG TCTTTATGGA ATTATGACTA TGCTCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATTACAA TGATGGCTCC TGTTGAATCA 
AAGTTTTCCC AGTCAACTGG AGATATAGAT AAAGTGACAG ATACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ATTATGGCTT CAAACTGAGA AAAATGGACC ATGAGCCTGT GATAAAATGT 
AGAATGATGG AGGACTTGAG GAAATTTGAG GAAAACAATG GCTCTGACCT ACTGGCTGAT 
GAAAACTTCC TGATGGTGAC ACAGCTGCAC TGGGAGGATG ATATCATCTG GGATGGGGAG 
GATGTCAAGC ACAAAGGAAC AAAGCCTCAG CGTGCAAGCC TGGCAGGCTG GCTTCCTTCC 
AGCATGACTA GGAATGCCAT GGCCTACAAT GTTCAGCAAG GTTTTGCAGC CACCCTGGAT 
GATGACAAAC CTTGGTACTC CATCTTTCCC ATTGACAACG AGGAGCTGGT ATATGGACGC 
TGGGAGGACA ATATCATTTG GGATGCTCAG GCCATGCCCA GGCTGTTGGA GCCTCCTGTT 
TTGACACTTG ATCCCAATGA TGAGAACCTT ATTTTGGAAA TTCCTGATGA AAAGGAAGAG 
GCCACTTCTA ACTCCCCCTC CAAGGAAAAT AAGAAAGAAT CATCTCTGAA GAAGAGCCGA 
ATTCTCTTAG GGAAAACAGG GGTCATCAAG GAGGAACCAC AGCAGAACAT GTCTCAGCCA 
GAAGTGAAAG ATCCATGGAA TCTCTCCAAT GATGAATATT ACTACCCCAA GCAACAGGGT 
CTTCGAGGCA CCTTTGGAGG AAATATTATC CAGCACTCAA TTCCTGCAGT GGAATTACGA 
CAGCCCTTCT TTCCTACCCA TATGGGGCCC ATCAAACTTC GGCAGTTCCA TCGTCCACCC 
CTGAAGAAAT ATTCCTTCGG GGCACTCTCT CAGCCAGGTC CCCACTCAGT CCAGCCCTTG 
CTGAAGCACA TCAAAAAGAA GGCTAAGATG AGAGAACAAG AGAGACAAGC TTCTGGTGGT 
GGAGAGATGT TTTTTATGCG CACACCTCAG GACCTCACAG GCAAAGATGG AGATCTTATT 
CTTGCAGAAT ACAGTGAGGA AAATGGACCA TTAATGATGC AGGTTGGCAT GGCAACCAAG 
ATAAAAAACT ATTATAAGCG GAAACCTGGA AAAGATCCTG GGGCCCCAGA TTGTAAATAT 
GGAGAAACTG TTTACTGTCA TACATCTCCT TTCCTAGGCT CTCTTCATCC TGGTCAATTA 
CTGCAGGCAT TTGAGAACAA TCTCTTTCGT GCCCCAATTT ATCTTCATAA GATGCCAGAA 
ACTGACTTCC TTATTATTCG GACAAGACAA GGTTACTACA TTCGGGAGTT AGTGGATATT 
TTTGTGGTCG GCCAGCAATG CCCCTTGTTT GAAGTTCCTG GGCCCAACTC CAAAAGAGCT 
AATACACATA TTCGAGACTT TCTCCAGGTT TTTATTTACC GCCTCTTCTG GAAGAGTAAA 
GATCGGCCAA GGCGGATCCG AATGGAAGAT ATAAAAAAAG CCTTTCCTTC TCACTCAGAA 
AGTAGCATCC GCAAGAGGCT AAAGCTCTGC GCTGACTTCA AACGCACAGG GATGGACTCA 
AACTGGTGGG TGCTAAAGTC AGATTTTCGT TTACCAACTG AAGAGGAGAT CAGAGCTATG 
GTGTCTCCAG AGCAGTGCTG TGCTTATTAT AGCATGATAG CTGCAGAGCA GCGACTAAAG 
GATGCTGGCT ATGGTGAGAA GTCCTTTTTT GCTCCAGAAG AAGAAAATGA GGAAGATTTC 
CAGATGAAGA TTGATGATGA AGTTCGCACT GCTCCTTGGA ATACCACAAG GGCTTTCATT 
GCTGCCATGA AGGGCAAGTG TCTCTTGGAG GTGACTGGGG TGGCAGATCC CACAGGGTGT 
GGTGAAGGAT TCTCCTATGT CAAGATTCCA AACAAACCAA CACAGCAGAA GGATGATAAG 
GAGCCTCAGC CAGTGAAGAA GACAGTGACA GGAACAGATG CAGATCTCCG TCGCCTCTCA 
CTGAAAAATG CCAAGCAGCT CCTACGTAAA TTTGGTGTGC CTGAGGAAGA GATTAAAAAG 
CTGTCCCGCT GGGAAGTGAT TGATGTGGTA CGCACAATGT CAACAGAGCA AGCCCGCTCT 
GGAGAGGGGC CCATGAGTAA ATTTGCCCGT GGATCAAGGT TTTCTGTGGC TGAGCATCAA 
GAGCGTTACA AAGAGGAATG TCAGCGCATC TTTGATCTAC AGAACAAGGT TTTGTCATCA 
ACTGAAATAT TGTCAACTGA CACAGACAGC AGCTCAGCCG AAGACAGTGA CTTTGAAGAA 
ATGGGAAAGA ACATTGAGAA CATGTTGCAG AATAAGAAAA CCAGCTCTCA GCTGTCACGT 
GAACGAGAGG AGCAGGAGCG GAAGGAACTG CAGCGGATGC TACTGGCAGG TTCTGCAGCA 
TCAGGAAACA ATCACAGAGA TGATGATACA GCTTCTGTGA CCAGCCTTAA CTCTTCTGCC 
ACTGGCCGTT GTCTCAAAAT TTATCGCACA TTTCGAGATG AAGAAGGGAA AGAGTATGTT 
CGCTGTGAGA CAGTCCGAAA GCCTGCTGTC ATTGATGCCT ATGTGCGCAT ACGGACCACA 
AAAGATGAGG AATTCATTCG AAAGTTTGCC CTTTTTGATG AACAGCATCG AGAAGAGATG 
CGAAAGGAAC GGCGGAGGAT TCAAGAGCAA CTTAGGCGGC TCAAGCGGAA TCAGGAAAAG 
GAGAAGCTTA AGGGTCCCCC TGAGAAGAAG CCTAAAAAAA TGAAGGAGCG TCCTGATCTA 
AAACTGAAAT GTGGAGCTTG TGGTGCCATT GGGCACATGA GGACAAACAA GTTCTGCCCC 
CTCTACTATC AAACAAATGC TCCACCTTCC AACCCTGTTG CCATGACAGA GGAGCAGGAG 
GAGGAGTTGG AAAAGACAGT CATTCATAAT GATAATGAAG AACTTATCAA GGTTGAAGGG 
ACCAAGATCG TCCTGGGAAA ACAGCTAATT GAGAGTGCAG ATGAGGTTCG AAGAAAATCT 
CTGGTTCTCA AGTTCCCTAA ACAGCAACTT CCTCCCAAGA AGAAACGGCG AGTTGGAACC 
ACTGTTCACT GTGACTACTT AAATAGACCT CATAAATCCA TCCACCGGCG CCGGACAGAC 
CCAATGGTGA CATTGTCCTC CATCTTGGAG TCTATCATCA ATGACATGAG AGATCTTCCA 
AATACCTACC CTTTCCACAC TCCAGTCAAT GCAAAGGTTG TAAAGGACTA CTACAAAATC 
ATCACTCGAC CAATGGACTT ACAAACACTC CGTGAAAATG TGCGCAAACG CCTTTACCCA 
TCTCGGGAAG AGTTCAGAGA ACATTTGGAG CTCATTGTGA AAAATAGTGC AACCTATAAT 
GGTCCGAAGC ACTCATTGAC CCAGATCTCT CAATCTATGC TGGATCTCTG TGATGAAAAA 
CTCAAAGAGA AAGAAGATAA ATTGGCTCGT TTAGAGAAAG CTATCAACCC CTTGCTGGAT 
GATGATGACC AAGTAGCATT TTCTTTCATT CTGGACAACA TTGTCACCCA GAAAATGATG 
GCAGTTCCAG ATTCTTGGCC ATTTCATCAC CCAGTTAATA AGAAGTTTGT TCCAGATTAT 
TACAAAGTGA TTATCAGTCC AATGGATTTA GAGACTATAC GTAAGAATAT CTCCAAGCAC 
AAGTACCAGA GTCGGGAGAG CTTTCTGGAT GATGTCAACC TTATTCTGGC CAACAGTGTT 
AAGTATAATG GGCCTGAAAG TCAGTATACT AAGACTGCTC AGGAGATTGT AAATGTCTGT 
TACCAGACAT TGACTGAGTA TGATGAACAT TTGACTCAAC TTGAGAAGGA TATTTGTACA 
GCTAAGGAAG CGGCTTTGGA GGAAGCAGAA TTAGAAAGCT TGGACCCAAT GACCCCAGGG 
CCTTATACAC CTCAGGCTAA GCCTCCTGAT TTGTATGACA CCAACACATC CCTCAGTATG 
TCCCGAGATG CCTCTGTATT TCAAGATGAG AGCAATATGT CTGTCCTGGA TATTCCCACT 
GCCACTCCAG AAAAGCAGGT GACACAGATG CGCCAGGGCC GAGGTAGGCT GGGCGAGGAA 
GACTCTGATG TAGATATTGA AGGGTATGAT GATGAGGAGG AGGATGGGAA ACCTAAGACT 
CCAGCCCCAG AAGGTGAAGA TGCAGATGGT GATCTTGCAG ATGAAGAGGA AGGAAGTATG 
CAACAGCCTC AAGCCAGTGT CCTGTATGAG GATTTGCTTA TGTCTGAAGG AGAAGATGAT 
GAAGAAGATG CTGGGAGTGA TGAAGAAGGA GACAATCCTT TCTCTGCTAT CCAACTGAGT 
GAAAGTGGAA GTGACTCTGA TGTTGGATCT AGTGGGATAA GACCCAAACA GCCCCGCATG 
CTTCAGGAAA ACACAAGGAT GGGCATGGAA AATGAAGAAA GCATGATGTC CTATGAGGGA 
GATGGTGGGG AGGCTTCTCA TGGTTTGGAG GATAGCAATA TGAGTTATGG GAGCTATGAG 
GAGCCTGATC CCAAGTCGAA CACCCAAGAT ACAAGCTTCA GCAGCATCGG TGGGTATGAG 
GTATCAGAGG AAGAAGAAGA TGAGGAGGAA GAGCAGCGCT CTGGGCCAAG TGTACTAAGC 
CAGGTCCACC TGTCAGAGGA TGAGGAGGAC AGTGAGGATT TCCACTCCAT TGCTGGGGAC 
AGTGATTTGG ACTCTGATGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAF1 ( XM_005641473.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005641530.1 Canis lupus familiaris TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa (TAF1), transcript variant X1, mRNA.

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CloneID	OCe32527
Clone ID Related Accession (Same CDS sequence)	XM_005641473.3 , XM_005641473.2
Accession Version	XM_005641473.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5784bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	transcription initiation factor TFIID subunit 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005641473.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACCTG GCTGGGATTT GCTGCTGCCT GTAGCCGCCG GAGACACTAC CAGCGCCATC 
ATGTCAGACA CGGACAGCGA TGAAGATTCT GCTGGAGGCG GCCCATTTTC TCTAACCGGT 
TTCCTTTTTG GCAACATCAA TGGAGCCGGG CAGCTGGAGG GGGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGTC TGGGCAGCCT GATCACTGAA 
CTCACGGCAA ACGAAGAATT GGCTGGGACC GATGGTGCCC TGGTAAATGA TGAAGGATGG 
ATCAGGAGTA CAGAAGATGC TGTGGACTAT TCAGACATCA CTGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ATCAGCAGAC AATGGGGAGC TTGCAGCCCC TTTGCCACTC AGATTATGAT 
GAAGATGACT ATGATGCCGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAATGAA GAAAGATAAG GACCAGGATG GTCTTACCGG TGTGTCTGAG 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCATCACAG 
GCAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA ACACGATGCC 
ACCAAGCTGT TGCCAAGTGT CACAGAACTT TTCCCGGAAT TTCGGCCTGG GAAGGTATTA 
CGCTTCCTTC GTCTTTTCGG ACCAGGGAAG AATGTCCCAT CTGTTTGGCG GAGTGCTCGG 
AGAAAGAGGA AGAAGAAGCA CCGTGAGCTG ATACAGGAAG AACAGATCCA GGAGGTGGAG 
TGCTCAGTAG AGTCCGAAGT CAACAAGAAG TCTTTATGGA ATTATGACTA TGCTCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATTACAA TGATGGCTCC TGTTGAATCA 
AAGTTTTCCC AGTCAACTGG AGATATAGAT AAAGTGACAG ATACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ATTATGGCTT CAAACTGAGA AAAATGGACC ATGAGCCTGT GATAAAATGT 
AGAATGATGG AGGACTTGAG GAAATTTGAG GAAAACAATG GCTCTGACCT ACTGGCTGAT 
GAAAACTTCC TGATGGTGAC ACAGCTGCAC TGGGAGGATG ATATCATCTG GGATGGGGAG 
GATGTCAAGC ACAAAGGAAC AAAGCCTCAG CGTGCAAGCC TGGCAGGCTG GCTTCCTTCC 
AGCATGACTA GGAATGCCAT GGCCTACAAT GTTCAGCAAG GTTTTGCAGC CACCCTGGAT 
GATGACAAAC CTTGGTACTC CATCTTTCCC ATTGACAACG AGGAGCTGGT ATATGGACGC 
TGGGAGGACA ATATCATTTG GGATGCTCAG GCCATGCCCA GGCTGTTGGA GCCTCCTGTT 
TTGACACTTG ATCCCAATGA TGAGAACCTT ATTTTGGAAA TTCCTGATGA AAAGGAAGAG 
GCCACTTCTA ACTCCCCCTC CAAGGAAAAT AAGAAAGAAT CATCTCTGAA GAAGAGCCGA 
ATTCTCTTAG GGAAAACAGG GGTCATCAAG GAGGAACCAC AGCAGAACAT GTCTCAGCCA 
GAAGTGAAAG ATCCATGGAA TCTCTCCAAT GATGAATATT ACTACCCCAA GCAACAGGGT 
CTTCGAGGCA CCTTTGGAGG AAATATTATC CAGCACTCAA TTCCTGCAGT GGAATTACGA 
CAGCCCTTCT TTCCTACCCA TATGGGGCCC ATCAAACTTC GGCAGTTCCA TCGTCCACCC 
CTGAAGAAAT ATTCCTTCGG GGCACTCTCT CAGCCAGGTC CCCACTCAGT CCAGCCCTTG 
CTGAAGCACA TCAAAAAGAA GGCTAAGATG AGAGAACAAG AGAGACAAGC TTCTGGTGGT 
GGAGAGATGT TTTTTATGCG CACACCTCAG GACCTCACAG GCAAAGATGG AGATCTTATT 
CTTGCAGAAT ACAGTGAGGA AAATGGACCA TTAATGATGC AGGTTGGCAT GGCAACCAAG 
ATAAAAAACT ATTATAAGCG GAAACCTGGA AAAGATCCTG GGGCCCCAGA TTGTAAATAT 
GGAGAAACTG TTTACTGTCA TACATCTCCT TTCCTAGGCT CTCTTCATCC TGGTCAATTA 
CTGCAGGCAT TTGAGAACAA TCTCTTTCGT GCCCCAATTT ATCTTCATAA GATGCCAGAA 
ACTGACTTCC TTATTATTCG GACAAGACAA GGTTACTACA TTCGGGAGTT AGTGGATATT 
TTTGTGGTCG GCCAGCAATG CCCCTTGTTT GAAGTTCCTG GGCCCAACTC CAAAAGAGCT 
AATACACATA TTCGAGACTT TCTCCAGGTT TTTATTTACC GCCTCTTCTG GAAGAGTAAA 
GATCGGCCAA GGCGGATCCG AATGGAAGAT ATAAAAAAAG CCTTTCCTTC TCACTCAGAA 
AGTAGCATCC GCAAGAGGCT AAAGCTCTGC GCTGACTTCA AACGCACAGG GATGGACTCA 
AACTGGTGGG TGCTAAAGTC AGATTTTCGT TTACCAACTG AAGAGGAGAT CAGAGCTATG 
GTGTCTCCAG AGCAGTGCTG TGCTTATTAT AGCATGATAG CTGCAGAGCA GCGACTAAAG 
GATGCTGGCT ATGGTGAGAA GTCCTTTTTT GCTCCAGAAG AAGAAAATGA GGAAGATTTC 
CAGATGAAGA TTGATGATGA AGTTCGCACT GCTCCTTGGA ATACCACAAG GGCTTTCATT 
GCTGCCATGA AGGGCAAGTG TCTCTTGGAG GTGACTGGGG TGGCAGATCC CACAGGGTGT 
GGTGAAGGAT TCTCCTATGT CAAGATTCCA AACAAACCAA CACAGCAGAA GGATGATAAG 
GAGCCTCAGC CAGTGAAGAA GACAGTGACA GGAACAGATG CAGATCTCCG TCGCCTCTCA 
CTGAAAAATG CCAAGCAGCT CCTACGTAAA TTTGGTGTGC CTGAGGAAGA GATTAAAAAG 
CTGTCCCGCT GGGAAGTGAT TGATGTGGTA CGCACAATGT CAACAGAGCA AGCCCGCTCT 
GGAGAGGGGC CCATGAGTAA ATTTGCCCGT GGATCAAGGT TTTCTGTGGC TGAGCATCAA 
GAGCGTTACA AAGAGGAATG TCAGCGCATC TTTGATCTAC AGAACAAGGT TTTGTCATCA 
ACTGAAATAT TGTCAACTGA CACAGACAGC AGCTCAGCCG AAGACAGTGA CTTTGAAGAA 
ATGGGAAAGA ACATTGAGAA CATGTTGCAG AATAAGAAAA CCAGCTCTCA GCTGTCACGT 
GAACGAGAGG AGCAGGAGCG GAAGGAACTG CAGCGGATGC TACTGGCAGG TTCTGCAGCA 
TCAGGAAACA ATCACAGAGA TGATGATACA GCTTCTGTGA CCAGCCTTAA CTCTTCTGCC 
ACTGGCCGTT GTCTCAAAAT TTATCGCACA TTTCGAGATG AAGAAGGGAA AGAGTATGTT 
CGCTGTGAGA CAGTCCGAAA GCCTGCTGTC ATTGATGCCT ATGTGCGCAT ACGGACCACA 
AAAGATGAGG AATTCATTCG AAAGTTTGCC CTTTTTGATG AACAGCATCG AGAAGAGATG 
CGAAAGGAAC GGCGGAGGAT TCAAGAGCAA CTTAGGCGGC TCAAGCGGAA TCAGGAAAAG 
GAGAAGCTTA AGGGTCCCCC TGAGAAGAAG CCTAAAAAAA TGAAGGAGCG TCCTGATCTA 
AAACTGAAAT GTGGAGCTTG TGGTGCCATT GGGCACATGA GGACAAACAA GTTCTGCCCC 
CTCTACTATC AAACAAATGC TCCACCTTCC AACCCTGTTG CCATGACAGA GGAGCAGGAG 
GAGGAGTTGG AAAAGACAGT CATTCATAAT GATAATGAAG AACTTATCAA GGTTGAAGGG 
ACCAAGATCG TCCTGGGAAA ACAGCTAATT GAGAGTGCAG ATGAGGTTCG AAGAAAATCT 
CTGGTTCTCA AGTTCCCTAA ACAGCAACTT CCTCCCAAGA AGAAACGGCG AGTTGGAACC 
ACTGTTCACT GTGACTACTT AAATAGACCT CATAAATCCA TCCACCGGCG CCGGACAGAC 
CCAATGGTGA CATTGTCCTC CATCTTGGAG TCTATCATCA ATGACATGAG AGATCTTCCA 
AATACCTACC CTTTCCACAC TCCAGTCAAT GCAAAGGTTG TAAAGGACTA CTACAAAATC 
ATCACTCGAC CAATGGACTT ACAAACACTC CGTGAAAATG TGCGCAAACG CCTTTACCCA 
TCTCGGGAAG AGTTCAGAGA ACATTTGGAG CTCATTGTGA AAAATAGTGC AACCTATAAT 
GGTCCGAAGC ACTCATTGAC CCAGATCTCT CAATCTATGC TGGATCTCTG TGATGAAAAA 
CTCAAAGAGA AAGAAGATAA ATTGGCTCGT TTAGAGAAAG CTATCAACCC CTTGCTGGAT 
GATGATGACC AAGTAGCATT TTCTTTCATT CTGGACAACA TTGTCACCCA GAAAATGATG 
GCAGTTCCAG ATTCTTGGCC ATTTCATCAC CCAGTTAATA AGAAGTTTGT TCCAGATTAT 
TACAAAGTGA TTATCAGTCC AATGGATTTA GAGACTATAC GTAAGAATAT CTCCAAGCAC 
AAGTACCAGA GTCGGGAGAG CTTTCTGGAT GATGTCAACC TTATTCTGGC CAACAGTGTT 
AAGTATAATG GGCCTGAAAG TCAGTATACT AAGACTGCTC AGGAGATTGT AAATGTCTGT 
TACCAGACAT TGACTGAGTA TGATGAACAT TTGACTCAAC TTGAGAAGGA TATTTGTACA 
GCTAAGGAAG CGGCTTTGGA GGAAGCAGAA TTAGAAAGCT TGGACCCAAT GACCCCAGGG 
CCTTATACAC CTCAGGCTAA GCCTCCTGAT TTGTATGACA CCAACACATC CCTCAGTATG 
TCCCGAGATG CCTCTGTATT TCAAGATGAG AGCAATATGT CTGTCCTGGA TATTCCCACT 
GCCACTCCAG AAAAGCAGGT GACACAGATG CGCCAGGGCC GAGGTAGGCT GGGCGAGGAA 
GACTCTGATG TAGATATTGA AGGGTATGAT GATGAGGAGG AGGATGGGAA ACCTAAGACT 
CCAGCCCCAG AAGGTGAAGA TGCAGATGGT GATCTTGCAG ATGAAGAGGA AGGAAGTATG 
CAACAGCCTC AAGCCAGTGT CCTGTATGAG GATTTGCTTA TGTCTGAAGG AGAAGATGAT 
GAAGAAGATG CTGGGAGTGA TGAAGAAGGA GACAATCCTT TCTCTGCTAT CCAACTGAGT 
GAAAGTGGAA GTGACTCTGA TGTTGGATCT AGTGGGATAA GACCCAAACA GCCCCGCATG 
CTTCAGGAAA ACACAAGGAT GGGCATGGAA AATGAAGAAA GCATGATGTC CTATGAGGGA 
GATGGTGGGG AGGCTTCTCA TGGTTTGGAG GATAGCAATA TGAGTTATGG GAGCTATGAG 
GAGCCTGATC CCAAGTCGAA CACCCAAGAT ACAAGCTTCA GCAGCATCGG TGGGTATGAG 
GTATCAGAGG AAGAAGAAGA TGAGGAGGAA GAGCAGCGCT CTGGGCCAAG TGTACTAAGC 
CAGGTCCACC TGTCAGAGGA TGAGGAGGAC AGTGAGGATT TCCACTCCAT TGCTGGGGAC 
AGTGATTTGG ACTCTGATGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005641530.1
CDS	93..5876
Translation	MGPGWDLLLPVAAGDTTSAIMSDTDSDEDSAGGGPFSLTGFLFGNINGAGQLEGESVLDDECKKHLAGLGALGLGSLITELTANEELAGTDGALVNDEGWIRSTEDAVDYSDITEVAEDESRRYQQTMGSLQPLCHSDYDEDDYDADCEDIDCKLMPPPPPPPGPMKKDKDQDGLTGVSEDGEGIILPSIIAPSSLASEKVDFSSSSDSESEMGPQEASQAESEDGKLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSARRKRKKKHRELIQEEQIQEVECSVESEVNKKSLWNYDYAPPPPPEQCLSDDEITMMAPVESKFSQSTGDIDKVTDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKLRKMDHEPVIKCRMMEDLRKFEENNGSDLLADENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDKPWYSIFPIDNEELVYGRWEDNIIWDAQAMPRLLEPPVLTLDPNDENLILEIPDEKEEATSNSPSKENKKESSLKKSRILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYYIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYYSMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEILSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMLLAGSAASGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKPAVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIISPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNGPESQYTKTAQEIVNVCYQTLTEYDEHLTQLEKDICTAKEAALEEAELESLDPMTPGPYTPQAKPPDLYDTNTSLSMSRDASVFQDESNMSVLDIPTATPEKQVTQMRQGRGRLGEEDSDVDIEGYDDEEEDGKPKTPAPEGEDADGDLADEEEGSMQQPQASVLYEDLLMSEGEDDEEDAGSDEEGDNPFSAIQLSESGSDSDVGSSGIRPKQPRMLQENTRMGMENEESMMSYEGDGGEASHGLEDSNMSYGSYEEPDPKSNTQDTSFSSIGGYEVSEEEEDEEEEQRSGPSVLSQVHLSEDEEDSEDFHSIAGDSDLDSDE

Target ORF information:

RefSeq Version	XM_005641473.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa (TAF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005641473.2

ORF Insert Sequence:

ATGGGACCTG GCTGGGATTT GCTGCTGCCT GTAGCCGCCG GAGACACTAC CAGCGCCATC 
ATGTCAGACA CGGACAGCGA TGAAGATTCT GCTGGAGGCG GCCCATTTTC TCTAACCGGT 
TTCCTTTTTG GCAACATCAA TGGAGCCGGG CAGCTGGAGG GGGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGTC TGGGCAGCCT GATCACTGAA 
CTCACGGCAA ACGAAGAATT GGCTGGGACC GATGGTGCCC TGGTAAATGA TGAAGGATGG 
ATCAGGAGTA CAGAAGATGC TGTGGACTAT TCAGACATCA CTGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ATCAGCAGAC AATGGGGAGC TTGCAGCCCC TTTGCCACTC AGATTATGAT 
GAAGATGACT ATGATGCCGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAATGAA GAAAGATAAG GACCAGGATG GTCTTACCGG TGTGTCTGAG 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCATCACAG 
GCAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA ACACGATGCC 
ACCAAGCTGT TGCCAAGTGT CACAGAACTT TTCCCGGAAT TTCGGCCTGG GAAGGTATTA 
CGCTTCCTTC GTCTTTTCGG ACCAGGGAAG AATGTCCCAT CTGTTTGGCG GAGTGCTCGG 
AGAAAGAGGA AGAAGAAGCA CCGTGAGCTG ATACAGGAAG AACAGATCCA GGAGGTGGAG 
TGCTCAGTAG AGTCCGAAGT CAACAAGAAG TCTTTATGGA ATTATGACTA TGCTCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATTACAA TGATGGCTCC TGTTGAATCA 
AAGTTTTCCC AGTCAACTGG AGATATAGAT AAAGTGACAG ATACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ATTATGGCTT CAAACTGAGA AAAATGGACC ATGAGCCTGT GATAAAATGT 
AGAATGATGG AGGACTTGAG GAAATTTGAG GAAAACAATG GCTCTGACCT ACTGGCTGAT 
GAAAACTTCC TGATGGTGAC ACAGCTGCAC TGGGAGGATG ATATCATCTG GGATGGGGAG 
GATGTCAAGC ACAAAGGAAC AAAGCCTCAG CGTGCAAGCC TGGCAGGCTG GCTTCCTTCC 
AGCATGACTA GGAATGCCAT GGCCTACAAT GTTCAGCAAG GTTTTGCAGC CACCCTGGAT 
GATGACAAAC CTTGGTACTC CATCTTTCCC ATTGACAACG AGGAGCTGGT ATATGGACGC 
TGGGAGGACA ATATCATTTG GGATGCTCAG GCCATGCCCA GGCTGTTGGA GCCTCCTGTT 
TTGACACTTG ATCCCAATGA TGAGAACCTT ATTTTGGAAA TTCCTGATGA AAAGGAAGAG 
GCCACTTCTA ACTCCCCCTC CAAGGAAAAT AAGAAAGAAT CATCTCTGAA GAAGAGCCGA 
ATTCTCTTAG GGAAAACAGG GGTCATCAAG GAGGAACCAC AGCAGAACAT GTCTCAGCCA 
GAAGTGAAAG ATCCATGGAA TCTCTCCAAT GATGAATATT ACTACCCCAA GCAACAGGGT 
CTTCGAGGCA CCTTTGGAGG AAATATTATC CAGCACTCAA TTCCTGCAGT GGAATTACGA 
CAGCCCTTCT TTCCTACCCA TATGGGGCCC ATCAAACTTC GGCAGTTCCA TCGTCCACCC 
CTGAAGAAAT ATTCCTTCGG GGCACTCTCT CAGCCAGGTC CCCACTCAGT CCAGCCCTTG 
CTGAAGCACA TCAAAAAGAA GGCTAAGATG AGAGAACAAG AGAGACAAGC TTCTGGTGGT 
GGAGAGATGT TTTTTATGCG CACACCTCAG GACCTCACAG GCAAAGATGG AGATCTTATT 
CTTGCAGAAT ACAGTGAGGA AAATGGACCA TTAATGATGC AGGTTGGCAT GGCAACCAAG 
ATAAAAAACT ATTATAAGCG GAAACCTGGA AAAGATCCTG GGGCCCCAGA TTGTAAATAT 
GGAGAAACTG TTTACTGTCA TACATCTCCT TTCCTAGGCT CTCTTCATCC TGGTCAATTA 
CTGCAGGCAT TTGAGAACAA TCTCTTTCGT GCCCCAATTT ATCTTCATAA GATGCCAGAA 
ACTGACTTCC TTATTATTCG GACAAGACAA GGTTACTACA TTCGGGAGTT AGTGGATATT 
TTTGTGGTCG GCCAGCAATG CCCCTTGTTT GAAGTTCCTG GGCCCAACTC CAAAAGAGCT 
AATACACATA TTCGAGACTT TCTCCAGGTT TTTATTTACC GCCTCTTCTG GAAGAGTAAA 
GATCGGCCAA GGCGGATCCG AATGGAAGAT ATAAAAAAAG CCTTTCCTTC TCACTCAGAA 
AGTAGCATCC GCAAGAGGCT AAAGCTCTGC GCTGACTTCA AACGCACAGG GATGGACTCA 
AACTGGTGGG TGCTAAAGTC AGATTTTCGT TTACCAACTG AAGAGGAGAT CAGAGCTATG 
GTGTCTCCAG AGCAGTGCTG TGCTTATTAT AGCATGATAG CTGCAGAGCA GCGACTAAAG 
GATGCTGGCT ATGGTGAGAA GTCCTTTTTT GCTCCAGAAG AAGAAAATGA GGAAGATTTC 
CAGATGAAGA TTGATGATGA AGTTCGCACT GCTCCTTGGA ATACCACAAG GGCTTTCATT 
GCTGCCATGA AGGGCAAGTG TCTCTTGGAG GTGACTGGGG TGGCAGATCC CACAGGGTGT 
GGTGAAGGAT TCTCCTATGT CAAGATTCCA AACAAACCAA CACAGCAGAA GGATGATAAG 
GAGCCTCAGC CAGTGAAGAA GACAGTGACA GGAACAGATG CAGATCTCCG TCGCCTCTCA 
CTGAAAAATG CCAAGCAGCT CCTACGTAAA TTTGGTGTGC CTGAGGAAGA GATTAAAAAG 
CTGTCCCGCT GGGAAGTGAT TGATGTGGTA CGCACAATGT CAACAGAGCA AGCCCGCTCT 
GGAGAGGGGC CCATGAGTAA ATTTGCCCGT GGATCAAGGT TTTCTGTGGC TGAGCATCAA 
GAGCGTTACA AAGAGGAATG TCAGCGCATC TTTGATCTAC AGAACAAGGT TTTGTCATCA 
ACTGAAATAT TGTCAACTGA CACAGACAGC AGCTCAGCCG AAGACAGTGA CTTTGAAGAA 
ATGGGAAAGA ACATTGAGAA CATGTTGCAG AATAAGAAAA CCAGCTCTCA GCTGTCACGT 
GAACGAGAGG AGCAGGAGCG GAAGGAACTG CAGCGGATGC TACTGGCAGG TTCTGCAGCA 
TCAGGAAACA ATCACAGAGA TGATGATACA GCTTCTGTGA CCAGCCTTAA CTCTTCTGCC 
ACTGGCCGTT GTCTCAAAAT TTATCGCACA TTTCGAGATG AAGAAGGGAA AGAGTATGTT 
CGCTGTGAGA CAGTCCGAAA GCCTGCTGTC ATTGATGCCT ATGTGCGCAT ACGGACCACA 
AAAGATGAGG AATTCATTCG AAAGTTTGCC CTTTTTGATG AACAGCATCG AGAAGAGATG 
CGAAAGGAAC GGCGGAGGAT TCAAGAGCAA CTTAGGCGGC TCAAGCGGAA TCAGGAAAAG 
GAGAAGCTTA AGGGTCCCCC TGAGAAGAAG CCTAAAAAAA TGAAGGAGCG TCCTGATCTA 
AAACTGAAAT GTGGAGCTTG TGGTGCCATT GGGCACATGA GGACAAACAA GTTCTGCCCC 
CTCTACTATC AAACAAATGC TCCACCTTCC AACCCTGTTG CCATGACAGA GGAGCAGGAG 
GAGGAGTTGG AAAAGACAGT CATTCATAAT GATAATGAAG AACTTATCAA GGTTGAAGGG 
ACCAAGATCG TCCTGGGAAA ACAGCTAATT GAGAGTGCAG ATGAGGTTCG AAGAAAATCT 
CTGGTTCTCA AGTTCCCTAA ACAGCAACTT CCTCCCAAGA AGAAACGGCG AGTTGGAACC 
ACTGTTCACT GTGACTACTT AAATAGACCT CATAAATCCA TCCACCGGCG CCGGACAGAC 
CCAATGGTGA CATTGTCCTC CATCTTGGAG TCTATCATCA ATGACATGAG AGATCTTCCA 
AATACCTACC CTTTCCACAC TCCAGTCAAT GCAAAGGTTG TAAAGGACTA CTACAAAATC 
ATCACTCGAC CAATGGACTT ACAAACACTC CGTGAAAATG TGCGCAAACG CCTTTACCCA 
TCTCGGGAAG AGTTCAGAGA ACATTTGGAG CTCATTGTGA AAAATAGTGC AACCTATAAT 
GGTCCGAAGC ACTCATTGAC CCAGATCTCT CAATCTATGC TGGATCTCTG TGATGAAAAA 
CTCAAAGAGA AAGAAGATAA ATTGGCTCGT TTAGAGAAAG CTATCAACCC CTTGCTGGAT 
GATGATGACC AAGTAGCATT TTCTTTCATT CTGGACAACA TTGTCACCCA GAAAATGATG 
GCAGTTCCAG ATTCTTGGCC ATTTCATCAC CCAGTTAATA AGAAGTTTGT TCCAGATTAT 
TACAAAGTGA TTATCAGTCC AATGGATTTA GAGACTATAC GTAAGAATAT CTCCAAGCAC 
AAGTACCAGA GTCGGGAGAG CTTTCTGGAT GATGTCAACC TTATTCTGGC CAACAGTGTT 
AAGTATAATG GGCCTGAAAG TCAGTATACT AAGACTGCTC AGGAGATTGT AAATGTCTGT 
TACCAGACAT TGACTGAGTA TGATGAACAT TTGACTCAAC TTGAGAAGGA TATTTGTACA 
GCTAAGGAAG CGGCTTTGGA GGAAGCAGAA TTAGAAAGCT TGGACCCAAT GACCCCAGGG 
CCTTATACAC CTCAGGCTAA GCCTCCTGAT TTGTATGACA CCAACACATC CCTCAGTATG 
TCCCGAGATG CCTCTGTATT TCAAGATGAG AGCAATATGT CTGTCCTGGA TATTCCCACT 
GCCACTCCAG AAAAGCAGGT GACACAGATG CGCCAGGGCC GAGGTAGGCT GGGCGAGGAA 
GACTCTGATG TAGATATTGA AGGGTATGAT GATGAGGAGG AGGATGGGAA ACCTAAGACT 
CCAGCCCCAG AAGGTGAAGA TGCAGATGGT GATCTTGCAG ATGAAGAGGA AGGAAGTATG 
CAACAGCCTC AAGCCAGTGT CCTGTATGAG GATTTGCTTA TGTCTGAAGG AGAAGATGAT 
GAAGAAGATG CTGGGAGTGA TGAAGAAGGA GACAATCCTT TCTCTGCTAT CCAACTGAGT 
GAAAGTGGAA GTGACTCTGA TGTTGGATCT AGTGGGATAA GACCCAAACA GCCCCGCATG 
CTTCAGGAAA ACACAAGGAT GGGCATGGAA AATGAAGAAA GCATGATGTC CTATGAGGGA 
GATGGTGGGG AGGCTTCTCA TGGTTTGGAG GATAGCAATA TGAGTTATGG GAGCTATGAG 
GAGCCTGATC CCAAGTCGAA CACCCAAGAT ACAAGCTTCA GCAGCATCGG TGGGTATGAG 
GTATCAGAGG AAGAAGAAGA TGAGGAGGAA GAGCAGCGCT CTGGGCCAAG TGTACTAAGC 
CAGGTCCACC TGTCAGAGGA TGAGGAGGAC AGTGAGGATT TCCACTCCAT TGCTGGGGAC 
AGTGATTTGG ACTCTGATGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAF1 ( XM_005641474.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005641531.1 Canis lupus familiaris TATA-box binding protein associated factor 1 (TAF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe32528
Clone ID Related Accession (Same CDS sequence)	XM_005641474.3 , XM_005641474.2
Accession Version	XM_005641474.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	transcription initiation factor TFIID subunit 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005641474.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACCTG GCTGGGATTT GCTGCTGCCT GTAGCCGCCG GAGACACTAC CAGCGCCATC 
ATGTCAGACA CGGACAGCGA TGAAGATTCT GCTGGAGGCG GCCCATTTTC TCTAACCGGT 
TTCCTTTTTG GCAACATCAA TGGAGCCGGG CAGCTGGAGG GGGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGTC TGGGCAGCCT GATCACTGAA 
CTCACGGCAA ACGAAGAATT GGCTGGGACC GATGGTGCCC TGGTAAATGA TGAAGGATGG 
ATCAGGAGTA CAGAAGATGC TGTGGACTAT TCAGACATCA CTGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ATCAGCAGAC AATGGGGAGC TTGCAGCCCC TTTGCCACTC AGATTATGAT 
GAAGATGACT ATGATGCCGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAATGAA GAAAGATAAG GACCAGGATG GTCTTACCGG TGTGTCTGAG 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCATCACAG 
GCAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA ACACGATGCC 
ACCAAGCTGT TGCCAAGTGT CACAGAACTT TTCCCGGAAT TTCGGCCTGG GAAGGTATTA 
CGCTTCCTTC GTCTTTTCGG ACCAGGGAAG AATGTCCCAT CTGTTTGGCG GAGTGCTCGG 
AGAAAGAGGA AGAAGAAGCA CCGTGAGCTG ATACAGGAAG AACAGATCCA GGAGGTGGAG 
TGCTCAGTAG AGTCCGAAGT CAACAAGAAG TCTTTATGGA ATTATGACTA TGCTCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATTACAA TGATGGCTCC TGTTGAATCA 
AAGTTTTCCC AGTCAACTGG AGATATAGAT AAAGTGACAG ATACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ATTATGGCTT CAAACTGAGA AAAATGGACC ATGAGCCTGT GATAAAATGT 
AGAATGATGG AGGACTTGAG GAAATTTGAG GAAAACAATG GCTCTGACCT ACTGGCTGAT 
GAAAACTTCC TGATGGTGAC ACAGCTGCAC TGGGAGGATG ATATCATCTG GGATGGGGAG 
GATGTCAAGC ACAAAGGAAC AAAGCCTCAG CGTGCAAGCC TGGCAGGCTG GCTTCCTTCC 
AGCATGACTA GGAATGCCAT GGCCTACAAT GTTCAGCAAG GTTTTGCAGC CACCCTGGAT 
GATGACAAAC CTTGGTACTC CATCTTTCCC ATTGACAACG AGGAGCTGGT ATATGGACGC 
TGGGAGGACA ATATCATTTG GGATGCTCAG GCCATGCCCA GGCTGTTGGA GCCTCCTGTT 
TTGACACTTG ATCCCAATGA TGAGAACCTT ATTTTGGAAA TTCCTGATGA AAAGGAAGAG 
GCCACTTCTA ACTCCCCCTC CAAGGAAAAT AAGAAAGAAT CATCTCTGAA GAAGAGCCGA 
ATTCTCTTAG GGAAAACAGG GGTCATCAAG GAGGAACCAC AGCAGAACAT GTCTCAGCCA 
GAAGTGAAAG ATCCATGGAA TCTCTCCAAT GATGAATATT ACTACCCCAA GCAACAGGGT 
CTTCGAGGCA CCTTTGGAGG AAATATTATC CAGCACTCAA TTCCTGCAGT GGAATTACGA 
CAGCCCTTCT TTCCTACCCA TATGGGGCCC ATCAAACTTC GGCAGTTCCA TCGTCCACCC 
CTGAAGAAAT ATTCCTTCGG GGCACTCTCT CAGCCAGGTC CCCACTCAGT CCAGCCCTTG 
CTGAAGCACA TCAAAAAGAA GGCTAAGATG AGAGAACAAG AGAGACAAGC TTCTGGTGGT 
GGAGAGATGT TTTTTATGCG CACACCTCAG GACCTCACAG GCAAAGATGG AGATCTTATT 
CTTGCAGAAT ACAGTGAGGA AAATGGACCA TTAATGATGC AGGTTGGCAT GGCAACCAAG 
ATAAAAAACT ATTATAAGCG GAAACCTGGA AAAGATCCTG GGGCCCCAGA TTGTAAATAT 
GGAGAAACTG TTTACTGTCA TACATCTCCT TTCCTAGGCT CTCTTCATCC TGGTCAATTA 
CTGCAGGCAT TTGAGAACAA TCTCTTTCGT GCCCCAATTT ATCTTCATAA GATGCCAGAA 
ACTGACTTCC TTATTATTCG GACAAGACAA GGTTACTACA TTCGGGAGTT AGTGGATATT 
TTTGTGGTCG GCCAGCAATG CCCCTTGTTT GAAGTTCCTG GGCCCAACTC CAAAAGAGCT 
AATACACATA TTCGAGACTT TCTCCAGGTT TTTATTTACC GCCTCTTCTG GAAGAGTAAA 
GATCGGCCAA GGCGGATCCG AATGGAAGAT ATAAAAAAAG CCTTTCCTTC TCACTCAGAA 
AGTAGCATCC GCAAGAGGCT AAAGCTCTGC GCTGACTTCA AACGCACAGG GATGGACTCA 
AACTGGTGGG TGCTAAAGTC AGATTTTCGT TTACCAACTG AAGAGGAGAT CAGAGCTATG 
GTGTCTCCAG AGCAGTGCTG TGCTTATTAT AGCATGATAG CTGCAGAGCA GCGACTAAAG 
GATGCTGGCT ATGGTGAGAA GTCCTTTTTT GCTCCAGAAG AAGAAAATGA GGAAGATTTC 
CAGATGAAGA TTGATGATGA AGTTCGCACT GCTCCTTGGA ATACCACAAG GGCTTTCATT 
GCTGCCATGA AGGGCAAGTG TCTCTTGGAG GTGACTGGGG TGGCAGATCC CACAGGGTGT 
GGTGAAGGAT TCTCCTATGT CAAGATTCCA AACAAACCAA CACAGCAGAA GGATGATAAG 
GAGCCTCAGC CAGTGAAGAA GACAGTGACA GGAACAGATG CAGATCTCCG TCGCCTCTCA 
CTGAAAAATG CCAAGCAGCT CCTACGTAAA TTTGGTGTGC CTGAGGAAGA GATTAAAAAG 
CTGTCCCGCT GGGAAGTGAT TGATGTGGTA CGCACAATGT CAACAGAGCA AGCCCGCTCT 
GGAGAGGGGC CCATGAGTAA ATTTGCCCGT GGATCAAGGT TTTCTGTGGC TGAGCATCAA 
GAGCGTTACA AAGAGGAATG TCAGCGCATC TTTGATCTAC AGAACAAGGT TTTGTCATCA 
ACTGAAATAT TGTCAACTGA CACAGACAGC AGCTCAGCCG AAGACAGTGA CTTTGAAGAA 
ATGGGAAAGA ACATTGAGAA CATGTTGCAG AATAAGAAAA CCAGCTCTCA GCTGTCACGT 
GAACGAGAGG AGCAGGAGCG GAAGGAACTG CAGCGGATGC TACTGGCAGC AGGTTCTGCA 
GCATCAGGAA ACAATCACAG AGATGATGAT ACAGCTTCTG TGACCAGCCT TAACTCTTCT 
GCCACTGGCC GTTGTCTCAA AATTTATCGC ACATTTCGAG ATGAAGAAGG GAAAGAGTAT 
GTTCGCTGTG AGACAGTCCG AAAGCCTGCT GTCATTGATG CCTATGTGCG CATACGGACC 
ACAAAAGATG AGGAATTCAT TCGAAAGTTT GCCCTTTTTG ATGAACAGCA TCGAGAAGAG 
ATGCGAAAGG AACGGCGGAG GATTCAAGAG CAACTTAGGC GGCTCAAGCG GAATCAGGAA 
AAGGAGAAGC TTAAGGGTCC CCCTGAGAAG AAGCCTAAAA AAATGAAGGA GCGTCCTGAT 
CTAAAACTGA AATGTGGAGC TTGTGGTGCC ATTGGGCACA TGAGGACAAA CAAGTTCTGC 
CCCCTCTACT ATCAAACAAA TGCTCCACCT TCCAACCCTG TTGCCATGAC AGAGGAGCAG 
GAGGAGGAGT TGGAAAAGAC AGTCATTCAT AATGATAATG AAGAACTTAT CAAGGTTGAA 
GGGACCAAGA TCGTCCTGGG AAAACAGCTA ATTGAGAGTG CAGATGAGGT TCGAAGAAAA 
TCTCTGGTTC TCAAGTTCCC TAAACAGCAA CTTCCTCCCA AGAAGAAACG GCGAGTTGGA 
ACCACTGTTC ACTGTGACTA CTTAAATAGA CCTCATAAAT CCATCCACCG GCGCCGGACA 
GACCCAATGG TGACATTGTC CTCCATCTTG GAGTCTATCA TCAATGACAT GAGAGATCTT 
CCAAATACCT ACCCTTTCCA CACTCCAGTC AATGCAAAGG TTGTAAAGGA CTACTACAAA 
ATCATCACTC GACCAATGGA CTTACAAACA CTCCGTGAAA ATGTGCGCAA ACGCCTTTAC 
CCATCTCGGG AAGAGTTCAG AGAACATTTG GAGCTCATTG TGAAAAATAG TGCAACCTAT 
AATGGTCCGA AGCACTCATT GACCCAGATC TCTCAATCTA TGCTGGATCT CTGTGATGAA 
AAACTCAAAG AGAAAGAAGA TAAATTGGCT CGTTTAGAGA AAGCTATCAA CCCCTTGCTG 
GATGATGATG ACCAAGTAGC ATTTTCTTTC ATTCTGGACA ACATTGTCAC CCAGAAAATG 
ATGGCAGTTC CAGATTCTTG GCCATTTCAT CACCCAGTTA ATAAGAAGTT TGTTCCAGAT 
TATTACAAAG TGATTATCAG TCCAATGGAT TTAGAGACTA TACGTAAGAA TATCTCCAAG 
CACAAGTACC AGAGTCGGGA GAGCTTTCTG GATGATGTCA ACCTTATTCT GGCCAACAGT 
GTTAAGTATA ATGGGCCTGA AAGTCAGTAT ACTAAGACTG CTCAGGAGAT TGTAAATGTC 
TGTTACCAGA CATTGACTGA GTATGATGAA CATTTGACTC AACTTGAGAA GGATATTTGT 
ACAGCTAAGG AAGCGGCTTT GGAGGAAGCA GAATTAGAAA GCTTGGACCC AATGACCCCA 
GGGCCTTATA CACCTCAGCC TCCTGATTTG TATGACACCA ACACATCCCT CAGTATGTCC 
CGAGATGCCT CTGTATTTCA AGATGAGAGC AATATGTCTG TCCTGGATAT TCCCACTGCC 
ACTCCAGAAA AGCAGGTGAC ACAGATGCGC CAGGGCCGAG GTAGGCTGGG CGAGGAAGAC 
TCTGATGTAG ATATTGAAGG GTATGATGAT GAGGAGGAGG ATGGGAAACC TAAGACTCCA 
GCCCCAGAAG GTGAAGATGC AGATGGTGAT CTTGCAGATG AAGAGGAAGG AAGTATGCAA 
CAGCCTCAAG CCAGTGTCCT GTATGAGGAT TTGCTTATGT CTGAAGGAGA AGATGATGAA 
GAAGATGCTG GGAGTGATGA AGAAGGAGAC AATCCTTTCT CTGCTATCCA ACTGAGTGAA 
AGTGGAAGTG ACTCTGATGT TGGATCTAGT GGGATAAGAC CCAAACAGCC CCGCATGCTT 
CAGGAAAACA CAAGGATGGG CATGGAAAAT GAAGAAAGCA TGATGTCCTA TGAGGGAGAT 
GGTGGGGAGG CTTCTCATGG TTTGGAGGAT AGCAATATGA GTTATGGGAG CTATGAGGAG 
CCTGATCCCA AGTCGAACAC CCAAGATACA AGCTTCAGCA GCATCGGTGG GTATGAGGTA 
TCAGAGGAAG AAGAAGATGA GGAGGAAGAG CAGCGCTCTG GGCCAAGTGT ACTAAGCCAG 
GTCCACCTGT CAGAGGATGA GGAGGACAGT GAGGATTTCC ACTCCATTGC TGGGGACAGT 
GATTTGGACT CTGATGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005641531.1
CDS	1..5781
Translation	MGPGWDLLLPVAAGDTTSAIMSDTDSDEDSAGGGPFSLTGFLFGNINGAGQLEGESVLDDECKKHLAGLGALGLGSLITELTANEELAGTDGALVNDEGWIRSTEDAVDYSDITEVAEDESRRYQQTMGSLQPLCHSDYDEDDYDADCEDIDCKLMPPPPPPPGPMKKDKDQDGLTGVSEDGEGIILPSIIAPSSLASEKVDFSSSSDSESEMGPQEASQAESEDGKLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSARRKRKKKHRELIQEEQIQEVECSVESEVNKKSLWNYDYAPPPPPEQCLSDDEITMMAPVESKFSQSTGDIDKVTDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKLRKMDHEPVIKCRMMEDLRKFEENNGSDLLADENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDKPWYSIFPIDNEELVYGRWEDNIIWDAQAMPRLLEPPVLTLDPNDENLILEIPDEKEEATSNSPSKENKKESSLKKSRILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYYIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYYSMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEILSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMLLAAGSAASGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKPAVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIISPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNGPESQYTKTAQEIVNVCYQTLTEYDEHLTQLEKDICTAKEAALEEAELESLDPMTPGPYTPQPPDLYDTNTSLSMSRDASVFQDESNMSVLDIPTATPEKQVTQMRQGRGRLGEEDSDVDIEGYDDEEEDGKPKTPAPEGEDADGDLADEEEGSMQQPQASVLYEDLLMSEGEDDEEDAGSDEEGDNPFSAIQLSESGSDSDVGSSGIRPKQPRMLQENTRMGMENEESMMSYEGDGGEASHGLEDSNMSYGSYEEPDPKSNTQDTSFSSIGGYEVSEEEEDEEEEQRSGPSVLSQVHLSEDEEDSEDFHSIAGDSDLDSDE

Target ORF information:

RefSeq Version	XM_005641474.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris TATA-box binding protein associated factor 1 (TAF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005641474.3

ORF Insert Sequence:

ATGGGACCTG GCTGGGATTT GCTGCTGCCT GTAGCCGCCG GAGACACTAC CAGCGCCATC 
ATGTCAGACA CGGACAGCGA TGAAGATTCT GCTGGAGGCG GCCCATTTTC TCTAACCGGT 
TTCCTTTTTG GCAACATCAA TGGAGCCGGG CAGCTGGAGG GGGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGTC TGGGCAGCCT GATCACTGAA 
CTCACGGCAA ACGAAGAATT GGCTGGGACC GATGGTGCCC TGGTAAATGA TGAAGGATGG 
ATCAGGAGTA CAGAAGATGC TGTGGACTAT TCAGACATCA CTGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ATCAGCAGAC AATGGGGAGC TTGCAGCCCC TTTGCCACTC AGATTATGAT 
GAAGATGACT ATGATGCCGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAATGAA GAAAGATAAG GACCAGGATG GTCTTACCGG TGTGTCTGAG 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCATCACAG 
GCAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA ACACGATGCC 
ACCAAGCTGT TGCCAAGTGT CACAGAACTT TTCCCGGAAT TTCGGCCTGG GAAGGTATTA 
CGCTTCCTTC GTCTTTTCGG ACCAGGGAAG AATGTCCCAT CTGTTTGGCG GAGTGCTCGG 
AGAAAGAGGA AGAAGAAGCA CCGTGAGCTG ATACAGGAAG AACAGATCCA GGAGGTGGAG 
TGCTCAGTAG AGTCCGAAGT CAACAAGAAG TCTTTATGGA ATTATGACTA TGCTCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATTACAA TGATGGCTCC TGTTGAATCA 
AAGTTTTCCC AGTCAACTGG AGATATAGAT AAAGTGACAG ATACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ATTATGGCTT CAAACTGAGA AAAATGGACC ATGAGCCTGT GATAAAATGT 
AGAATGATGG AGGACTTGAG GAAATTTGAG GAAAACAATG GCTCTGACCT ACTGGCTGAT 
GAAAACTTCC TGATGGTGAC ACAGCTGCAC TGGGAGGATG ATATCATCTG GGATGGGGAG 
GATGTCAAGC ACAAAGGAAC AAAGCCTCAG CGTGCAAGCC TGGCAGGCTG GCTTCCTTCC 
AGCATGACTA GGAATGCCAT GGCCTACAAT GTTCAGCAAG GTTTTGCAGC CACCCTGGAT 
GATGACAAAC CTTGGTACTC CATCTTTCCC ATTGACAACG AGGAGCTGGT ATATGGACGC 
TGGGAGGACA ATATCATTTG GGATGCTCAG GCCATGCCCA GGCTGTTGGA GCCTCCTGTT 
TTGACACTTG ATCCCAATGA TGAGAACCTT ATTTTGGAAA TTCCTGATGA AAAGGAAGAG 
GCCACTTCTA ACTCCCCCTC CAAGGAAAAT AAGAAAGAAT CATCTCTGAA GAAGAGCCGA 
ATTCTCTTAG GGAAAACAGG GGTCATCAAG GAGGAACCAC AGCAGAACAT GTCTCAGCCA 
GAAGTGAAAG ATCCATGGAA TCTCTCCAAT GATGAATATT ACTACCCCAA GCAACAGGGT 
CTTCGAGGCA CCTTTGGAGG AAATATTATC CAGCACTCAA TTCCTGCAGT GGAATTACGA 
CAGCCCTTCT TTCCTACCCA TATGGGGCCC ATCAAACTTC GGCAGTTCCA TCGTCCACCC 
CTGAAGAAAT ATTCCTTCGG GGCACTCTCT CAGCCAGGTC CCCACTCAGT CCAGCCCTTG 
CTGAAGCACA TCAAAAAGAA GGCTAAGATG AGAGAACAAG AGAGACAAGC TTCTGGTGGT 
GGAGAGATGT TTTTTATGCG CACACCTCAG GACCTCACAG GCAAAGATGG AGATCTTATT 
CTTGCAGAAT ACAGTGAGGA AAATGGACCA TTAATGATGC AGGTTGGCAT GGCAACCAAG 
ATAAAAAACT ATTATAAGCG GAAACCTGGA AAAGATCCTG GGGCCCCAGA TTGTAAATAT 
GGAGAAACTG TTTACTGTCA TACATCTCCT TTCCTAGGCT CTCTTCATCC TGGTCAATTA 
CTGCAGGCAT TTGAGAACAA TCTCTTTCGT GCCCCAATTT ATCTTCATAA GATGCCAGAA 
ACTGACTTCC TTATTATTCG GACAAGACAA GGTTACTACA TTCGGGAGTT AGTGGATATT 
TTTGTGGTCG GCCAGCAATG CCCCTTGTTT GAAGTTCCTG GGCCCAACTC CAAAAGAGCT 
AATACACATA TTCGAGACTT TCTCCAGGTT TTTATTTACC GCCTCTTCTG GAAGAGTAAA 
GATCGGCCAA GGCGGATCCG AATGGAAGAT ATAAAAAAAG CCTTTCCTTC TCACTCAGAA 
AGTAGCATCC GCAAGAGGCT AAAGCTCTGC GCTGACTTCA AACGCACAGG GATGGACTCA 
AACTGGTGGG TGCTAAAGTC AGATTTTCGT TTACCAACTG AAGAGGAGAT CAGAGCTATG 
GTGTCTCCAG AGCAGTGCTG TGCTTATTAT AGCATGATAG CTGCAGAGCA GCGACTAAAG 
GATGCTGGCT ATGGTGAGAA GTCCTTTTTT GCTCCAGAAG AAGAAAATGA GGAAGATTTC 
CAGATGAAGA TTGATGATGA AGTTCGCACT GCTCCTTGGA ATACCACAAG GGCTTTCATT 
GCTGCCATGA AGGGCAAGTG TCTCTTGGAG GTGACTGGGG TGGCAGATCC CACAGGGTGT 
GGTGAAGGAT TCTCCTATGT CAAGATTCCA AACAAACCAA CACAGCAGAA GGATGATAAG 
GAGCCTCAGC CAGTGAAGAA GACAGTGACA GGAACAGATG CAGATCTCCG TCGCCTCTCA 
CTGAAAAATG CCAAGCAGCT CCTACGTAAA TTTGGTGTGC CTGAGGAAGA GATTAAAAAG 
CTGTCCCGCT GGGAAGTGAT TGATGTGGTA CGCACAATGT CAACAGAGCA AGCCCGCTCT 
GGAGAGGGGC CCATGAGTAA ATTTGCCCGT GGATCAAGGT TTTCTGTGGC TGAGCATCAA 
GAGCGTTACA AAGAGGAATG TCAGCGCATC TTTGATCTAC AGAACAAGGT TTTGTCATCA 
ACTGAAATAT TGTCAACTGA CACAGACAGC AGCTCAGCCG AAGACAGTGA CTTTGAAGAA 
ATGGGAAAGA ACATTGAGAA CATGTTGCAG AATAAGAAAA CCAGCTCTCA GCTGTCACGT 
GAACGAGAGG AGCAGGAGCG GAAGGAACTG CAGCGGATGC TACTGGCAGC AGGTTCTGCA 
GCATCAGGAA ACAATCACAG AGATGATGAT ACAGCTTCTG TGACCAGCCT TAACTCTTCT 
GCCACTGGCC GTTGTCTCAA AATTTATCGC ACATTTCGAG ATGAAGAAGG GAAAGAGTAT 
GTTCGCTGTG AGACAGTCCG AAAGCCTGCT GTCATTGATG CCTATGTGCG CATACGGACC 
ACAAAAGATG AGGAATTCAT TCGAAAGTTT GCCCTTTTTG ATGAACAGCA TCGAGAAGAG 
ATGCGAAAGG AACGGCGGAG GATTCAAGAG CAACTTAGGC GGCTCAAGCG GAATCAGGAA 
AAGGAGAAGC TTAAGGGTCC CCCTGAGAAG AAGCCTAAAA AAATGAAGGA GCGTCCTGAT 
CTAAAACTGA AATGTGGAGC TTGTGGTGCC ATTGGGCACA TGAGGACAAA CAAGTTCTGC 
CCCCTCTACT ATCAAACAAA TGCTCCACCT TCCAACCCTG TTGCCATGAC AGAGGAGCAG 
GAGGAGGAGT TGGAAAAGAC AGTCATTCAT AATGATAATG AAGAACTTAT CAAGGTTGAA 
GGGACCAAGA TCGTCCTGGG AAAACAGCTA ATTGAGAGTG CAGATGAGGT TCGAAGAAAA 
TCTCTGGTTC TCAAGTTCCC TAAACAGCAA CTTCCTCCCA AGAAGAAACG GCGAGTTGGA 
ACCACTGTTC ACTGTGACTA CTTAAATAGA CCTCATAAAT CCATCCACCG GCGCCGGACA 
GACCCAATGG TGACATTGTC CTCCATCTTG GAGTCTATCA TCAATGACAT GAGAGATCTT 
CCAAATACCT ACCCTTTCCA CACTCCAGTC AATGCAAAGG TTGTAAAGGA CTACTACAAA 
ATCATCACTC GACCAATGGA CTTACAAACA CTCCGTGAAA ATGTGCGCAA ACGCCTTTAC 
CCATCTCGGG AAGAGTTCAG AGAACATTTG GAGCTCATTG TGAAAAATAG TGCAACCTAT 
AATGGTCCGA AGCACTCATT GACCCAGATC TCTCAATCTA TGCTGGATCT CTGTGATGAA 
AAACTCAAAG AGAAAGAAGA TAAATTGGCT CGTTTAGAGA AAGCTATCAA CCCCTTGCTG 
GATGATGATG ACCAAGTAGC ATTTTCTTTC ATTCTGGACA ACATTGTCAC CCAGAAAATG 
ATGGCAGTTC CAGATTCTTG GCCATTTCAT CACCCAGTTA ATAAGAAGTT TGTTCCAGAT 
TATTACAAAG TGATTATCAG TCCAATGGAT TTAGAGACTA TACGTAAGAA TATCTCCAAG 
CACAAGTACC AGAGTCGGGA GAGCTTTCTG GATGATGTCA ACCTTATTCT GGCCAACAGT 
GTTAAGTATA ATGGGCCTGA AAGTCAGTAT ACTAAGACTG CTCAGGAGAT TGTAAATGTC 
TGTTACCAGA CATTGACTGA GTATGATGAA CATTTGACTC AACTTGAGAA GGATATTTGT 
ACAGCTAAGG AAGCGGCTTT GGAGGAAGCA GAATTAGAAA GCTTGGACCC AATGACCCCA 
GGGCCTTATA CACCTCAGCC TCCTGATTTG TATGACACCA ACACATCCCT CAGTATGTCC 
CGAGATGCCT CTGTATTTCA AGATGAGAGC AATATGTCTG TCCTGGATAT TCCCACTGCC 
ACTCCAGAAA AGCAGGTGAC ACAGATGCGC CAGGGCCGAG GTAGGCTGGG CGAGGAAGAC 
TCTGATGTAG ATATTGAAGG GTATGATGAT GAGGAGGAGG ATGGGAAACC TAAGACTCCA 
GCCCCAGAAG GTGAAGATGC AGATGGTGAT CTTGCAGATG AAGAGGAAGG AAGTATGCAA 
CAGCCTCAAG CCAGTGTCCT GTATGAGGAT TTGCTTATGT CTGAAGGAGA AGATGATGAA 
GAAGATGCTG GGAGTGATGA AGAAGGAGAC AATCCTTTCT CTGCTATCCA ACTGAGTGAA 
AGTGGAAGTG ACTCTGATGT TGGATCTAGT GGGATAAGAC CCAAACAGCC CCGCATGCTT 
CAGGAAAACA CAAGGATGGG CATGGAAAAT GAAGAAAGCA TGATGTCCTA TGAGGGAGAT 
GGTGGGGAGG CTTCTCATGG TTTGGAGGAT AGCAATATGA GTTATGGGAG CTATGAGGAG 
CCTGATCCCA AGTCGAACAC CCAAGATACA AGCTTCAGCA GCATCGGTGG GTATGAGGTA 
TCAGAGGAAG AAGAAGATGA GGAGGAAGAG CAGCGCTCTG GGCCAAGTGT ACTAAGCCAG 
GTCCACCTGT CAGAGGATGA GGAGGACAGT GAGGATTTCC ACTCCATTGC TGGGGACAGT 
GATTTGGACT CTGATGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAF1 ( XM_005641474.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005641531.1 Canis lupus familiaris TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa (TAF1), transcript variant X2, mRNA.

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CloneID	OCe32528
Clone ID Related Accession (Same CDS sequence)	XM_005641474.3 , XM_005641474.2
Accession Version	XM_005641474.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	transcription initiation factor TFIID subunit 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005641474.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACCTG GCTGGGATTT GCTGCTGCCT GTAGCCGCCG GAGACACTAC CAGCGCCATC 
ATGTCAGACA CGGACAGCGA TGAAGATTCT GCTGGAGGCG GCCCATTTTC TCTAACCGGT 
TTCCTTTTTG GCAACATCAA TGGAGCCGGG CAGCTGGAGG GGGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGTC TGGGCAGCCT GATCACTGAA 
CTCACGGCAA ACGAAGAATT GGCTGGGACC GATGGTGCCC TGGTAAATGA TGAAGGATGG 
ATCAGGAGTA CAGAAGATGC TGTGGACTAT TCAGACATCA CTGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ATCAGCAGAC AATGGGGAGC TTGCAGCCCC TTTGCCACTC AGATTATGAT 
GAAGATGACT ATGATGCCGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAATGAA GAAAGATAAG GACCAGGATG GTCTTACCGG TGTGTCTGAG 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCATCACAG 
GCAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA ACACGATGCC 
ACCAAGCTGT TGCCAAGTGT CACAGAACTT TTCCCGGAAT TTCGGCCTGG GAAGGTATTA 
CGCTTCCTTC GTCTTTTCGG ACCAGGGAAG AATGTCCCAT CTGTTTGGCG GAGTGCTCGG 
AGAAAGAGGA AGAAGAAGCA CCGTGAGCTG ATACAGGAAG AACAGATCCA GGAGGTGGAG 
TGCTCAGTAG AGTCCGAAGT CAACAAGAAG TCTTTATGGA ATTATGACTA TGCTCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATTACAA TGATGGCTCC TGTTGAATCA 
AAGTTTTCCC AGTCAACTGG AGATATAGAT AAAGTGACAG ATACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ATTATGGCTT CAAACTGAGA AAAATGGACC ATGAGCCTGT GATAAAATGT 
AGAATGATGG AGGACTTGAG GAAATTTGAG GAAAACAATG GCTCTGACCT ACTGGCTGAT 
GAAAACTTCC TGATGGTGAC ACAGCTGCAC TGGGAGGATG ATATCATCTG GGATGGGGAG 
GATGTCAAGC ACAAAGGAAC AAAGCCTCAG CGTGCAAGCC TGGCAGGCTG GCTTCCTTCC 
AGCATGACTA GGAATGCCAT GGCCTACAAT GTTCAGCAAG GTTTTGCAGC CACCCTGGAT 
GATGACAAAC CTTGGTACTC CATCTTTCCC ATTGACAACG AGGAGCTGGT ATATGGACGC 
TGGGAGGACA ATATCATTTG GGATGCTCAG GCCATGCCCA GGCTGTTGGA GCCTCCTGTT 
TTGACACTTG ATCCCAATGA TGAGAACCTT ATTTTGGAAA TTCCTGATGA AAAGGAAGAG 
GCCACTTCTA ACTCCCCCTC CAAGGAAAAT AAGAAAGAAT CATCTCTGAA GAAGAGCCGA 
ATTCTCTTAG GGAAAACAGG GGTCATCAAG GAGGAACCAC AGCAGAACAT GTCTCAGCCA 
GAAGTGAAAG ATCCATGGAA TCTCTCCAAT GATGAATATT ACTACCCCAA GCAACAGGGT 
CTTCGAGGCA CCTTTGGAGG AAATATTATC CAGCACTCAA TTCCTGCAGT GGAATTACGA 
CAGCCCTTCT TTCCTACCCA TATGGGGCCC ATCAAACTTC GGCAGTTCCA TCGTCCACCC 
CTGAAGAAAT ATTCCTTCGG GGCACTCTCT CAGCCAGGTC CCCACTCAGT CCAGCCCTTG 
CTGAAGCACA TCAAAAAGAA GGCTAAGATG AGAGAACAAG AGAGACAAGC TTCTGGTGGT 
GGAGAGATGT TTTTTATGCG CACACCTCAG GACCTCACAG GCAAAGATGG AGATCTTATT 
CTTGCAGAAT ACAGTGAGGA AAATGGACCA TTAATGATGC AGGTTGGCAT GGCAACCAAG 
ATAAAAAACT ATTATAAGCG GAAACCTGGA AAAGATCCTG GGGCCCCAGA TTGTAAATAT 
GGAGAAACTG TTTACTGTCA TACATCTCCT TTCCTAGGCT CTCTTCATCC TGGTCAATTA 
CTGCAGGCAT TTGAGAACAA TCTCTTTCGT GCCCCAATTT ATCTTCATAA GATGCCAGAA 
ACTGACTTCC TTATTATTCG GACAAGACAA GGTTACTACA TTCGGGAGTT AGTGGATATT 
TTTGTGGTCG GCCAGCAATG CCCCTTGTTT GAAGTTCCTG GGCCCAACTC CAAAAGAGCT 
AATACACATA TTCGAGACTT TCTCCAGGTT TTTATTTACC GCCTCTTCTG GAAGAGTAAA 
GATCGGCCAA GGCGGATCCG AATGGAAGAT ATAAAAAAAG CCTTTCCTTC TCACTCAGAA 
AGTAGCATCC GCAAGAGGCT AAAGCTCTGC GCTGACTTCA AACGCACAGG GATGGACTCA 
AACTGGTGGG TGCTAAAGTC AGATTTTCGT TTACCAACTG AAGAGGAGAT CAGAGCTATG 
GTGTCTCCAG AGCAGTGCTG TGCTTATTAT AGCATGATAG CTGCAGAGCA GCGACTAAAG 
GATGCTGGCT ATGGTGAGAA GTCCTTTTTT GCTCCAGAAG AAGAAAATGA GGAAGATTTC 
CAGATGAAGA TTGATGATGA AGTTCGCACT GCTCCTTGGA ATACCACAAG GGCTTTCATT 
GCTGCCATGA AGGGCAAGTG TCTCTTGGAG GTGACTGGGG TGGCAGATCC CACAGGGTGT 
GGTGAAGGAT TCTCCTATGT CAAGATTCCA AACAAACCAA CACAGCAGAA GGATGATAAG 
GAGCCTCAGC CAGTGAAGAA GACAGTGACA GGAACAGATG CAGATCTCCG TCGCCTCTCA 
CTGAAAAATG CCAAGCAGCT CCTACGTAAA TTTGGTGTGC CTGAGGAAGA GATTAAAAAG 
CTGTCCCGCT GGGAAGTGAT TGATGTGGTA CGCACAATGT CAACAGAGCA AGCCCGCTCT 
GGAGAGGGGC CCATGAGTAA ATTTGCCCGT GGATCAAGGT TTTCTGTGGC TGAGCATCAA 
GAGCGTTACA AAGAGGAATG TCAGCGCATC TTTGATCTAC AGAACAAGGT TTTGTCATCA 
ACTGAAATAT TGTCAACTGA CACAGACAGC AGCTCAGCCG AAGACAGTGA CTTTGAAGAA 
ATGGGAAAGA ACATTGAGAA CATGTTGCAG AATAAGAAAA CCAGCTCTCA GCTGTCACGT 
GAACGAGAGG AGCAGGAGCG GAAGGAACTG CAGCGGATGC TACTGGCAGC AGGTTCTGCA 
GCATCAGGAA ACAATCACAG AGATGATGAT ACAGCTTCTG TGACCAGCCT TAACTCTTCT 
GCCACTGGCC GTTGTCTCAA AATTTATCGC ACATTTCGAG ATGAAGAAGG GAAAGAGTAT 
GTTCGCTGTG AGACAGTCCG AAAGCCTGCT GTCATTGATG CCTATGTGCG CATACGGACC 
ACAAAAGATG AGGAATTCAT TCGAAAGTTT GCCCTTTTTG ATGAACAGCA TCGAGAAGAG 
ATGCGAAAGG AACGGCGGAG GATTCAAGAG CAACTTAGGC GGCTCAAGCG GAATCAGGAA 
AAGGAGAAGC TTAAGGGTCC CCCTGAGAAG AAGCCTAAAA AAATGAAGGA GCGTCCTGAT 
CTAAAACTGA AATGTGGAGC TTGTGGTGCC ATTGGGCACA TGAGGACAAA CAAGTTCTGC 
CCCCTCTACT ATCAAACAAA TGCTCCACCT TCCAACCCTG TTGCCATGAC AGAGGAGCAG 
GAGGAGGAGT TGGAAAAGAC AGTCATTCAT AATGATAATG AAGAACTTAT CAAGGTTGAA 
GGGACCAAGA TCGTCCTGGG AAAACAGCTA ATTGAGAGTG CAGATGAGGT TCGAAGAAAA 
TCTCTGGTTC TCAAGTTCCC TAAACAGCAA CTTCCTCCCA AGAAGAAACG GCGAGTTGGA 
ACCACTGTTC ACTGTGACTA CTTAAATAGA CCTCATAAAT CCATCCACCG GCGCCGGACA 
GACCCAATGG TGACATTGTC CTCCATCTTG GAGTCTATCA TCAATGACAT GAGAGATCTT 
CCAAATACCT ACCCTTTCCA CACTCCAGTC AATGCAAAGG TTGTAAAGGA CTACTACAAA 
ATCATCACTC GACCAATGGA CTTACAAACA CTCCGTGAAA ATGTGCGCAA ACGCCTTTAC 
CCATCTCGGG AAGAGTTCAG AGAACATTTG GAGCTCATTG TGAAAAATAG TGCAACCTAT 
AATGGTCCGA AGCACTCATT GACCCAGATC TCTCAATCTA TGCTGGATCT CTGTGATGAA 
AAACTCAAAG AGAAAGAAGA TAAATTGGCT CGTTTAGAGA AAGCTATCAA CCCCTTGCTG 
GATGATGATG ACCAAGTAGC ATTTTCTTTC ATTCTGGACA ACATTGTCAC CCAGAAAATG 
ATGGCAGTTC CAGATTCTTG GCCATTTCAT CACCCAGTTA ATAAGAAGTT TGTTCCAGAT 
TATTACAAAG TGATTATCAG TCCAATGGAT TTAGAGACTA TACGTAAGAA TATCTCCAAG 
CACAAGTACC AGAGTCGGGA GAGCTTTCTG GATGATGTCA ACCTTATTCT GGCCAACAGT 
GTTAAGTATA ATGGGCCTGA AAGTCAGTAT ACTAAGACTG CTCAGGAGAT TGTAAATGTC 
TGTTACCAGA CATTGACTGA GTATGATGAA CATTTGACTC AACTTGAGAA GGATATTTGT 
ACAGCTAAGG AAGCGGCTTT GGAGGAAGCA GAATTAGAAA GCTTGGACCC AATGACCCCA 
GGGCCTTATA CACCTCAGCC TCCTGATTTG TATGACACCA ACACATCCCT CAGTATGTCC 
CGAGATGCCT CTGTATTTCA AGATGAGAGC AATATGTCTG TCCTGGATAT TCCCACTGCC 
ACTCCAGAAA AGCAGGTGAC ACAGATGCGC CAGGGCCGAG GTAGGCTGGG CGAGGAAGAC 
TCTGATGTAG ATATTGAAGG GTATGATGAT GAGGAGGAGG ATGGGAAACC TAAGACTCCA 
GCCCCAGAAG GTGAAGATGC AGATGGTGAT CTTGCAGATG AAGAGGAAGG AAGTATGCAA 
CAGCCTCAAG CCAGTGTCCT GTATGAGGAT TTGCTTATGT CTGAAGGAGA AGATGATGAA 
GAAGATGCTG GGAGTGATGA AGAAGGAGAC AATCCTTTCT CTGCTATCCA ACTGAGTGAA 
AGTGGAAGTG ACTCTGATGT TGGATCTAGT GGGATAAGAC CCAAACAGCC CCGCATGCTT 
CAGGAAAACA CAAGGATGGG CATGGAAAAT GAAGAAAGCA TGATGTCCTA TGAGGGAGAT 
GGTGGGGAGG CTTCTCATGG TTTGGAGGAT AGCAATATGA GTTATGGGAG CTATGAGGAG 
CCTGATCCCA AGTCGAACAC CCAAGATACA AGCTTCAGCA GCATCGGTGG GTATGAGGTA 
TCAGAGGAAG AAGAAGATGA GGAGGAAGAG CAGCGCTCTG GGCCAAGTGT ACTAAGCCAG 
GTCCACCTGT CAGAGGATGA GGAGGACAGT GAGGATTTCC ACTCCATTGC TGGGGACAGT 
GATTTGGACT CTGATGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005641531.1
CDS	93..5873
Translation	MGPGWDLLLPVAAGDTTSAIMSDTDSDEDSAGGGPFSLTGFLFGNINGAGQLEGESVLDDECKKHLAGLGALGLGSLITELTANEELAGTDGALVNDEGWIRSTEDAVDYSDITEVAEDESRRYQQTMGSLQPLCHSDYDEDDYDADCEDIDCKLMPPPPPPPGPMKKDKDQDGLTGVSEDGEGIILPSIIAPSSLASEKVDFSSSSDSESEMGPQEASQAESEDGKLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSARRKRKKKHRELIQEEQIQEVECSVESEVNKKSLWNYDYAPPPPPEQCLSDDEITMMAPVESKFSQSTGDIDKVTDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKLRKMDHEPVIKCRMMEDLRKFEENNGSDLLADENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDKPWYSIFPIDNEELVYGRWEDNIIWDAQAMPRLLEPPVLTLDPNDENLILEIPDEKEEATSNSPSKENKKESSLKKSRILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYYIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYYSMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEILSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMLLAAGSAASGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKPAVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIISPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNGPESQYTKTAQEIVNVCYQTLTEYDEHLTQLEKDICTAKEAALEEAELESLDPMTPGPYTPQPPDLYDTNTSLSMSRDASVFQDESNMSVLDIPTATPEKQVTQMRQGRGRLGEEDSDVDIEGYDDEEEDGKPKTPAPEGEDADGDLADEEEGSMQQPQASVLYEDLLMSEGEDDEEDAGSDEEGDNPFSAIQLSESGSDSDVGSSGIRPKQPRMLQENTRMGMENEESMMSYEGDGGEASHGLEDSNMSYGSYEEPDPKSNTQDTSFSSIGGYEVSEEEEDEEEEQRSGPSVLSQVHLSEDEEDSEDFHSIAGDSDLDSDE

Target ORF information:

RefSeq Version	XM_005641474.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa (TAF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005641474.2

ORF Insert Sequence:

ATGGGACCTG GCTGGGATTT GCTGCTGCCT GTAGCCGCCG GAGACACTAC CAGCGCCATC 
ATGTCAGACA CGGACAGCGA TGAAGATTCT GCTGGAGGCG GCCCATTTTC TCTAACCGGT 
TTCCTTTTTG GCAACATCAA TGGAGCCGGG CAGCTGGAGG GGGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGTC TGGGCAGCCT GATCACTGAA 
CTCACGGCAA ACGAAGAATT GGCTGGGACC GATGGTGCCC TGGTAAATGA TGAAGGATGG 
ATCAGGAGTA CAGAAGATGC TGTGGACTAT TCAGACATCA CTGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ATCAGCAGAC AATGGGGAGC TTGCAGCCCC TTTGCCACTC AGATTATGAT 
GAAGATGACT ATGATGCCGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAATGAA GAAAGATAAG GACCAGGATG GTCTTACCGG TGTGTCTGAG 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCATCACAG 
GCAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA ACACGATGCC 
ACCAAGCTGT TGCCAAGTGT CACAGAACTT TTCCCGGAAT TTCGGCCTGG GAAGGTATTA 
CGCTTCCTTC GTCTTTTCGG ACCAGGGAAG AATGTCCCAT CTGTTTGGCG GAGTGCTCGG 
AGAAAGAGGA AGAAGAAGCA CCGTGAGCTG ATACAGGAAG AACAGATCCA GGAGGTGGAG 
TGCTCAGTAG AGTCCGAAGT CAACAAGAAG TCTTTATGGA ATTATGACTA TGCTCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATTACAA TGATGGCTCC TGTTGAATCA 
AAGTTTTCCC AGTCAACTGG AGATATAGAT AAAGTGACAG ATACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ATTATGGCTT CAAACTGAGA AAAATGGACC ATGAGCCTGT GATAAAATGT 
AGAATGATGG AGGACTTGAG GAAATTTGAG GAAAACAATG GCTCTGACCT ACTGGCTGAT 
GAAAACTTCC TGATGGTGAC ACAGCTGCAC TGGGAGGATG ATATCATCTG GGATGGGGAG 
GATGTCAAGC ACAAAGGAAC AAAGCCTCAG CGTGCAAGCC TGGCAGGCTG GCTTCCTTCC 
AGCATGACTA GGAATGCCAT GGCCTACAAT GTTCAGCAAG GTTTTGCAGC CACCCTGGAT 
GATGACAAAC CTTGGTACTC CATCTTTCCC ATTGACAACG AGGAGCTGGT ATATGGACGC 
TGGGAGGACA ATATCATTTG GGATGCTCAG GCCATGCCCA GGCTGTTGGA GCCTCCTGTT 
TTGACACTTG ATCCCAATGA TGAGAACCTT ATTTTGGAAA TTCCTGATGA AAAGGAAGAG 
GCCACTTCTA ACTCCCCCTC CAAGGAAAAT AAGAAAGAAT CATCTCTGAA GAAGAGCCGA 
ATTCTCTTAG GGAAAACAGG GGTCATCAAG GAGGAACCAC AGCAGAACAT GTCTCAGCCA 
GAAGTGAAAG ATCCATGGAA TCTCTCCAAT GATGAATATT ACTACCCCAA GCAACAGGGT 
CTTCGAGGCA CCTTTGGAGG AAATATTATC CAGCACTCAA TTCCTGCAGT GGAATTACGA 
CAGCCCTTCT TTCCTACCCA TATGGGGCCC ATCAAACTTC GGCAGTTCCA TCGTCCACCC 
CTGAAGAAAT ATTCCTTCGG GGCACTCTCT CAGCCAGGTC CCCACTCAGT CCAGCCCTTG 
CTGAAGCACA TCAAAAAGAA GGCTAAGATG AGAGAACAAG AGAGACAAGC TTCTGGTGGT 
GGAGAGATGT TTTTTATGCG CACACCTCAG GACCTCACAG GCAAAGATGG AGATCTTATT 
CTTGCAGAAT ACAGTGAGGA AAATGGACCA TTAATGATGC AGGTTGGCAT GGCAACCAAG 
ATAAAAAACT ATTATAAGCG GAAACCTGGA AAAGATCCTG GGGCCCCAGA TTGTAAATAT 
GGAGAAACTG TTTACTGTCA TACATCTCCT TTCCTAGGCT CTCTTCATCC TGGTCAATTA 
CTGCAGGCAT TTGAGAACAA TCTCTTTCGT GCCCCAATTT ATCTTCATAA GATGCCAGAA 
ACTGACTTCC TTATTATTCG GACAAGACAA GGTTACTACA TTCGGGAGTT AGTGGATATT 
TTTGTGGTCG GCCAGCAATG CCCCTTGTTT GAAGTTCCTG GGCCCAACTC CAAAAGAGCT 
AATACACATA TTCGAGACTT TCTCCAGGTT TTTATTTACC GCCTCTTCTG GAAGAGTAAA 
GATCGGCCAA GGCGGATCCG AATGGAAGAT ATAAAAAAAG CCTTTCCTTC TCACTCAGAA 
AGTAGCATCC GCAAGAGGCT AAAGCTCTGC GCTGACTTCA AACGCACAGG GATGGACTCA 
AACTGGTGGG TGCTAAAGTC AGATTTTCGT TTACCAACTG AAGAGGAGAT CAGAGCTATG 
GTGTCTCCAG AGCAGTGCTG TGCTTATTAT AGCATGATAG CTGCAGAGCA GCGACTAAAG 
GATGCTGGCT ATGGTGAGAA GTCCTTTTTT GCTCCAGAAG AAGAAAATGA GGAAGATTTC 
CAGATGAAGA TTGATGATGA AGTTCGCACT GCTCCTTGGA ATACCACAAG GGCTTTCATT 
GCTGCCATGA AGGGCAAGTG TCTCTTGGAG GTGACTGGGG TGGCAGATCC CACAGGGTGT 
GGTGAAGGAT TCTCCTATGT CAAGATTCCA AACAAACCAA CACAGCAGAA GGATGATAAG 
GAGCCTCAGC CAGTGAAGAA GACAGTGACA GGAACAGATG CAGATCTCCG TCGCCTCTCA 
CTGAAAAATG CCAAGCAGCT CCTACGTAAA TTTGGTGTGC CTGAGGAAGA GATTAAAAAG 
CTGTCCCGCT GGGAAGTGAT TGATGTGGTA CGCACAATGT CAACAGAGCA AGCCCGCTCT 
GGAGAGGGGC CCATGAGTAA ATTTGCCCGT GGATCAAGGT TTTCTGTGGC TGAGCATCAA 
GAGCGTTACA AAGAGGAATG TCAGCGCATC TTTGATCTAC AGAACAAGGT TTTGTCATCA 
ACTGAAATAT TGTCAACTGA CACAGACAGC AGCTCAGCCG AAGACAGTGA CTTTGAAGAA 
ATGGGAAAGA ACATTGAGAA CATGTTGCAG AATAAGAAAA CCAGCTCTCA GCTGTCACGT 
GAACGAGAGG AGCAGGAGCG GAAGGAACTG CAGCGGATGC TACTGGCAGC AGGTTCTGCA 
GCATCAGGAA ACAATCACAG AGATGATGAT ACAGCTTCTG TGACCAGCCT TAACTCTTCT 
GCCACTGGCC GTTGTCTCAA AATTTATCGC ACATTTCGAG ATGAAGAAGG GAAAGAGTAT 
GTTCGCTGTG AGACAGTCCG AAAGCCTGCT GTCATTGATG CCTATGTGCG CATACGGACC 
ACAAAAGATG AGGAATTCAT TCGAAAGTTT GCCCTTTTTG ATGAACAGCA TCGAGAAGAG 
ATGCGAAAGG AACGGCGGAG GATTCAAGAG CAACTTAGGC GGCTCAAGCG GAATCAGGAA 
AAGGAGAAGC TTAAGGGTCC CCCTGAGAAG AAGCCTAAAA AAATGAAGGA GCGTCCTGAT 
CTAAAACTGA AATGTGGAGC TTGTGGTGCC ATTGGGCACA TGAGGACAAA CAAGTTCTGC 
CCCCTCTACT ATCAAACAAA TGCTCCACCT TCCAACCCTG TTGCCATGAC AGAGGAGCAG 
GAGGAGGAGT TGGAAAAGAC AGTCATTCAT AATGATAATG AAGAACTTAT CAAGGTTGAA 
GGGACCAAGA TCGTCCTGGG AAAACAGCTA ATTGAGAGTG CAGATGAGGT TCGAAGAAAA 
TCTCTGGTTC TCAAGTTCCC TAAACAGCAA CTTCCTCCCA AGAAGAAACG GCGAGTTGGA 
ACCACTGTTC ACTGTGACTA CTTAAATAGA CCTCATAAAT CCATCCACCG GCGCCGGACA 
GACCCAATGG TGACATTGTC CTCCATCTTG GAGTCTATCA TCAATGACAT GAGAGATCTT 
CCAAATACCT ACCCTTTCCA CACTCCAGTC AATGCAAAGG TTGTAAAGGA CTACTACAAA 
ATCATCACTC GACCAATGGA CTTACAAACA CTCCGTGAAA ATGTGCGCAA ACGCCTTTAC 
CCATCTCGGG AAGAGTTCAG AGAACATTTG GAGCTCATTG TGAAAAATAG TGCAACCTAT 
AATGGTCCGA AGCACTCATT GACCCAGATC TCTCAATCTA TGCTGGATCT CTGTGATGAA 
AAACTCAAAG AGAAAGAAGA TAAATTGGCT CGTTTAGAGA AAGCTATCAA CCCCTTGCTG 
GATGATGATG ACCAAGTAGC ATTTTCTTTC ATTCTGGACA ACATTGTCAC CCAGAAAATG 
ATGGCAGTTC CAGATTCTTG GCCATTTCAT CACCCAGTTA ATAAGAAGTT TGTTCCAGAT 
TATTACAAAG TGATTATCAG TCCAATGGAT TTAGAGACTA TACGTAAGAA TATCTCCAAG 
CACAAGTACC AGAGTCGGGA GAGCTTTCTG GATGATGTCA ACCTTATTCT GGCCAACAGT 
GTTAAGTATA ATGGGCCTGA AAGTCAGTAT ACTAAGACTG CTCAGGAGAT TGTAAATGTC 
TGTTACCAGA CATTGACTGA GTATGATGAA CATTTGACTC AACTTGAGAA GGATATTTGT 
ACAGCTAAGG AAGCGGCTTT GGAGGAAGCA GAATTAGAAA GCTTGGACCC AATGACCCCA 
GGGCCTTATA CACCTCAGCC TCCTGATTTG TATGACACCA ACACATCCCT CAGTATGTCC 
CGAGATGCCT CTGTATTTCA AGATGAGAGC AATATGTCTG TCCTGGATAT TCCCACTGCC 
ACTCCAGAAA AGCAGGTGAC ACAGATGCGC CAGGGCCGAG GTAGGCTGGG CGAGGAAGAC 
TCTGATGTAG ATATTGAAGG GTATGATGAT GAGGAGGAGG ATGGGAAACC TAAGACTCCA 
GCCCCAGAAG GTGAAGATGC AGATGGTGAT CTTGCAGATG AAGAGGAAGG AAGTATGCAA 
CAGCCTCAAG CCAGTGTCCT GTATGAGGAT TTGCTTATGT CTGAAGGAGA AGATGATGAA 
GAAGATGCTG GGAGTGATGA AGAAGGAGAC AATCCTTTCT CTGCTATCCA ACTGAGTGAA 
AGTGGAAGTG ACTCTGATGT TGGATCTAGT GGGATAAGAC CCAAACAGCC CCGCATGCTT 
CAGGAAAACA CAAGGATGGG CATGGAAAAT GAAGAAAGCA TGATGTCCTA TGAGGGAGAT 
GGTGGGGAGG CTTCTCATGG TTTGGAGGAT AGCAATATGA GTTATGGGAG CTATGAGGAG 
CCTGATCCCA AGTCGAACAC CCAAGATACA AGCTTCAGCA GCATCGGTGG GTATGAGGTA 
TCAGAGGAAG AAGAAGATGA GGAGGAAGAG CAGCGCTCTG GGCCAAGTGT ACTAAGCCAG 
GTCCACCTGT CAGAGGATGA GGAGGACAGT GAGGATTTCC ACTCCATTGC TGGGGACAGT 
GATTTGGACT CTGATGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAF1 ( XM_005641475.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005641532.1 Canis lupus familiaris TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa (TAF1), transcript variant X3, mRNA.

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CloneID	OCe32529
Clone ID Related Accession (Same CDS sequence)	XM_005641475.2 , XM_005641475.3
Accession Version	XM_005641475.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5724bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	transcription initiation factor TFIID subunit 1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005641475.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACCTG GCTGGGATTT GCTGCTGCCT GTAGCCGCCG GAGACACTAC CAGCGCCATC 
ATGTCAGACA CGGACAGCGA TGAAGATTCT GCTGGAGGCG GCCCATTTTC TCTAACCGGT 
TTCCTTTTTG GCAACATCAA TGGAGCCGGG CAGCTGGAGG GGGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGTC TGGGCAGCCT GATCACTGAA 
CTCACGGCAA ACGAAGAATT GGCTGGGACC GATGGTGCCC TGGTAAATGA TGAAGGATGG 
ATCAGGAGTA CAGAAGATGC TGTGGACTAT TCAGACATCA CTGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ATCAGCAGAC AATGGGGAGC TTGCAGCCCC TTTGCCACTC AGATTATGAT 
GAAGATGACT ATGATGCCGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAATGAA GAAAGATAAG GACCAGGATG GTCTTACCGG TGAGAAAGTG 
GACTTCAGTA GTTCCTCTGA CTCAGAATCT GAGATGGGAC CTCAGGAAGC ATCACAGGCA 
GAATCTGAGG ATGGAAAGCT GACCCTACCA TTGGCTGGGA TTATGCAACA CGATGCCACC 
AAGCTGTTGC CAAGTGTCAC AGAACTTTTC CCGGAATTTC GGCCTGGGAA GGTATTACGC 
TTCCTTCGTC TTTTCGGACC AGGGAAGAAT GTCCCATCTG TTTGGCGGAG TGCTCGGAGA 
AAGAGGAAGA AGAAGCACCG TGAGCTGATA CAGGAAGAAC AGATCCAGGA GGTGGAGTGC 
TCAGTAGAGT CCGAAGTCAA CAAGAAGTCT TTATGGAATT ATGACTATGC TCCACCACCA 
CCTCCAGAGC AGTGTCTCTC TGATGATGAA ATTACAATGA TGGCTCCTGT TGAATCAAAG 
TTTTCCCAGT CAACTGGAGA TATAGATAAA GTGACAGATA CCAAACCAAG AGTGGCTGAG 
TGGCGTTATG GGCCTGCTCG ACTGTGGTAT GATATGCTGG GTGTCCCTGA AGATGGCAGT 
GGGTTTGATT ATGGCTTCAA ACTGAGAAAA ATGGACCATG AGCCTGTGAT AAAATGTAGA 
ATGATGGAGG ACTTGAGGAA ATTTGAGGAA AACAATGGCT CTGACCTACT GGCTGATGAA 
AACTTCCTGA TGGTGACACA GCTGCACTGG GAGGATGATA TCATCTGGGA TGGGGAGGAT 
GTCAAGCACA AAGGAACAAA GCCTCAGCGT GCAAGCCTGG CAGGCTGGCT TCCTTCCAGC 
ATGACTAGGA ATGCCATGGC CTACAATGTT CAGCAAGGTT TTGCAGCCAC CCTGGATGAT 
GACAAACCTT GGTACTCCAT CTTTCCCATT GACAACGAGG AGCTGGTATA TGGACGCTGG 
GAGGACAATA TCATTTGGGA TGCTCAGGCC ATGCCCAGGC TGTTGGAGCC TCCTGTTTTG 
ACACTTGATC CCAATGATGA GAACCTTATT TTGGAAATTC CTGATGAAAA GGAAGAGGCC 
ACTTCTAACT CCCCCTCCAA GGAAAATAAG AAAGAATCAT CTCTGAAGAA GAGCCGAATT 
CTCTTAGGGA AAACAGGGGT CATCAAGGAG GAACCACAGC AGAACATGTC TCAGCCAGAA 
GTGAAAGATC CATGGAATCT CTCCAATGAT GAATATTACT ACCCCAAGCA ACAGGGTCTT 
CGAGGCACCT TTGGAGGAAA TATTATCCAG CACTCAATTC CTGCAGTGGA ATTACGACAG 
CCCTTCTTTC CTACCCATAT GGGGCCCATC AAACTTCGGC AGTTCCATCG TCCACCCCTG 
AAGAAATATT CCTTCGGGGC ACTCTCTCAG CCAGGTCCCC ACTCAGTCCA GCCCTTGCTG 
AAGCACATCA AAAAGAAGGC TAAGATGAGA GAACAAGAGA GACAAGCTTC TGGTGGTGGA 
GAGATGTTTT TTATGCGCAC ACCTCAGGAC CTCACAGGCA AAGATGGAGA TCTTATTCTT 
GCAGAATACA GTGAGGAAAA TGGACCATTA ATGATGCAGG TTGGCATGGC AACCAAGATA 
AAAAACTATT ATAAGCGGAA ACCTGGAAAA GATCCTGGGG CCCCAGATTG TAAATATGGA 
GAAACTGTTT ACTGTCATAC ATCTCCTTTC CTAGGCTCTC TTCATCCTGG TCAATTACTG 
CAGGCATTTG AGAACAATCT CTTTCGTGCC CCAATTTATC TTCATAAGAT GCCAGAAACT 
GACTTCCTTA TTATTCGGAC AAGACAAGGT TACTACATTC GGGAGTTAGT GGATATTTTT 
GTGGTCGGCC AGCAATGCCC CTTGTTTGAA GTTCCTGGGC CCAACTCCAA AAGAGCTAAT 
ACACATATTC GAGACTTTCT CCAGGTTTTT ATTTACCGCC TCTTCTGGAA GAGTAAAGAT 
CGGCCAAGGC GGATCCGAAT GGAAGATATA AAAAAAGCCT TTCCTTCTCA CTCAGAAAGT 
AGCATCCGCA AGAGGCTAAA GCTCTGCGCT GACTTCAAAC GCACAGGGAT GGACTCAAAC 
TGGTGGGTGC TAAAGTCAGA TTTTCGTTTA CCAACTGAAG AGGAGATCAG AGCTATGGTG 
TCTCCAGAGC AGTGCTGTGC TTATTATAGC ATGATAGCTG CAGAGCAGCG ACTAAAGGAT 
GCTGGCTATG GTGAGAAGTC CTTTTTTGCT CCAGAAGAAG AAAATGAGGA AGATTTCCAG 
ATGAAGATTG ATGATGAAGT TCGCACTGCT CCTTGGAATA CCACAAGGGC TTTCATTGCT 
GCCATGAAGG GCAAGTGTCT CTTGGAGGTG ACTGGGGTGG CAGATCCCAC AGGGTGTGGT 
GAAGGATTCT CCTATGTCAA GATTCCAAAC AAACCAACAC AGCAGAAGGA TGATAAGGAG 
CCTCAGCCAG TGAAGAAGAC AGTGACAGGA ACAGATGCAG ATCTCCGTCG CCTCTCACTG 
AAAAATGCCA AGCAGCTCCT ACGTAAATTT GGTGTGCCTG AGGAAGAGAT TAAAAAGCTG 
TCCCGCTGGG AAGTGATTGA TGTGGTACGC ACAATGTCAA CAGAGCAAGC CCGCTCTGGA 
GAGGGGCCCA TGAGTAAATT TGCCCGTGGA TCAAGGTTTT CTGTGGCTGA GCATCAAGAG 
CGTTACAAAG AGGAATGTCA GCGCATCTTT GATCTACAGA ACAAGGTTTT GTCATCAACT 
GAAATATTGT CAACTGACAC AGACAGCAGC TCAGCCGAAG ACAGTGACTT TGAAGAAATG 
GGAAAGAACA TTGAGAACAT GTTGCAGAAT AAGAAAACCA GCTCTCAGCT GTCACGTGAA 
CGAGAGGAGC AGGAGCGGAA GGAACTGCAG CGGATGCTAC TGGCAGCAGG TTCTGCAGCA 
TCAGGAAACA ATCACAGAGA TGATGATACA GCTTCTGTGA CCAGCCTTAA CTCTTCTGCC 
ACTGGCCGTT GTCTCAAAAT TTATCGCACA TTTCGAGATG AAGAAGGGAA AGAGTATGTT 
CGCTGTGAGA CAGTCCGAAA GCCTGCTGTC ATTGATGCCT ATGTGCGCAT ACGGACCACA 
AAAGATGAGG AATTCATTCG AAAGTTTGCC CTTTTTGATG AACAGCATCG AGAAGAGATG 
CGAAAGGAAC GGCGGAGGAT TCAAGAGCAA CTTAGGCGGC TCAAGCGGAA TCAGGAAAAG 
GAGAAGCTTA AGGGTCCCCC TGAGAAGAAG CCTAAAAAAA TGAAGGAGCG TCCTGATCTA 
AAACTGAAAT GTGGAGCTTG TGGTGCCATT GGGCACATGA GGACAAACAA GTTCTGCCCC 
CTCTACTATC AAACAAATGC TCCACCTTCC AACCCTGTTG CCATGACAGA GGAGCAGGAG 
GAGGAGTTGG AAAAGACAGT CATTCATAAT GATAATGAAG AACTTATCAA GGTTGAAGGG 
ACCAAGATCG TCCTGGGAAA ACAGCTAATT GAGAGTGCAG ATGAGGTTCG AAGAAAATCT 
CTGGTTCTCA AGTTCCCTAA ACAGCAACTT CCTCCCAAGA AGAAACGGCG AGTTGGAACC 
ACTGTTCACT GTGACTACTT AAATAGACCT CATAAATCCA TCCACCGGCG CCGGACAGAC 
CCAATGGTGA CATTGTCCTC CATCTTGGAG TCTATCATCA ATGACATGAG AGATCTTCCA 
AATACCTACC CTTTCCACAC TCCAGTCAAT GCAAAGGTTG TAAAGGACTA CTACAAAATC 
ATCACTCGAC CAATGGACTT ACAAACACTC CGTGAAAATG TGCGCAAACG CCTTTACCCA 
TCTCGGGAAG AGTTCAGAGA ACATTTGGAG CTCATTGTGA AAAATAGTGC AACCTATAAT 
GGTCCGAAGC ACTCATTGAC CCAGATCTCT CAATCTATGC TGGATCTCTG TGATGAAAAA 
CTCAAAGAGA AAGAAGATAA ATTGGCTCGT TTAGAGAAAG CTATCAACCC CTTGCTGGAT 
GATGATGACC AAGTAGCATT TTCTTTCATT CTGGACAACA TTGTCACCCA GAAAATGATG 
GCAGTTCCAG ATTCTTGGCC ATTTCATCAC CCAGTTAATA AGAAGTTTGT TCCAGATTAT 
TACAAAGTGA TTATCAGTCC AATGGATTTA GAGACTATAC GTAAGAATAT CTCCAAGCAC 
AAGTACCAGA GTCGGGAGAG CTTTCTGGAT GATGTCAACC TTATTCTGGC CAACAGTGTT 
AAGTATAATG GGCCTGAAAG TCAGTATACT AAGACTGCTC AGGAGATTGT AAATGTCTGT 
TACCAGACAT TGACTGAGTA TGATGAACAT TTGACTCAAC TTGAGAAGGA TATTTGTACA 
GCTAAGGAAG CGGCTTTGGA GGAAGCAGAA TTAGAAAGCT TGGACCCAAT GACCCCAGGG 
CCTTATACAC CTCAGGCTAA GCCTCCTGAT TTGTATGACA CCAACACATC CCTCAGTATG 
TCCCGAGATG CCTCTGTATT TCAAGATGAG AGCAATATGT CTGTCCTGGA TATTCCCACT 
GCCACTCCAG AAAAGCAGGT GACACAGATG CGCCAGGGCC GAGGTAGGCT GGGCGAGGAA 
GACTCTGATG TAGATATTGA AGGGTATGAT GATGAGGAGG AGGATGGGAA ACCTAAGACT 
CCAGCCCCAG AAGGTGAAGA TGCAGATGGT GATCTTGCAG ATGAAGAGGA AGGAAGTATG 
CAACAGCCTC AAGCCAGTGT CCTGTATGAG GATTTGCTTA TGTCTGAAGG AGAAGATGAT 
GAAGAAGATG CTGGGAGTGA TGAAGAAGGA GACAATCCTT TCTCTGCTAT CCAACTGAGT 
GAAAGTGGAA GTGACTCTGA TGTTGGATCT AGTGGGATAA GACCCAAACA GCCCCGCATG 
CTTCAGGAAA ACACAAGGAT GGGCATGGAA AATGAAGAAA GCATGATGTC CTATGAGGGA 
GATGGTGGGG AGGCTTCTCA TGGTTTGGAG GATAGCAATA TGAGTTATGG GAGCTATGAG 
GAGCCTGATC CCAAGTCGAA CACCCAAGAT ACAAGCTTCA GCAGCATCGG TGGGTATGAG 
GTATCAGAGG AAGAAGAAGA TGAGGAGGAA GAGCAGCGCT CTGGGCCAAG TGTACTAAGC 
CAGGTCCACC TGTCAGAGGA TGAGGAGGAC AGTGAGGATT TCCACTCCAT TGCTGGGGAC 
AGTGATTTGG ACTCTGATGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005641532.1
CDS	93..5816
Translation	MGPGWDLLLPVAAGDTTSAIMSDTDSDEDSAGGGPFSLTGFLFGNINGAGQLEGESVLDDECKKHLAGLGALGLGSLITELTANEELAGTDGALVNDEGWIRSTEDAVDYSDITEVAEDESRRYQQTMGSLQPLCHSDYDEDDYDADCEDIDCKLMPPPPPPPGPMKKDKDQDGLTGEKVDFSSSSDSESEMGPQEASQAESEDGKLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSARRKRKKKHRELIQEEQIQEVECSVESEVNKKSLWNYDYAPPPPPEQCLSDDEITMMAPVESKFSQSTGDIDKVTDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKLRKMDHEPVIKCRMMEDLRKFEENNGSDLLADENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDKPWYSIFPIDNEELVYGRWEDNIIWDAQAMPRLLEPPVLTLDPNDENLILEIPDEKEEATSNSPSKENKKESSLKKSRILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYYIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYYSMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEILSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMLLAAGSAASGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKPAVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIISPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNGPESQYTKTAQEIVNVCYQTLTEYDEHLTQLEKDICTAKEAALEEAELESLDPMTPGPYTPQAKPPDLYDTNTSLSMSRDASVFQDESNMSVLDIPTATPEKQVTQMRQGRGRLGEEDSDVDIEGYDDEEEDGKPKTPAPEGEDADGDLADEEEGSMQQPQASVLYEDLLMSEGEDDEEDAGSDEEGDNPFSAIQLSESGSDSDVGSSGIRPKQPRMLQENTRMGMENEESMMSYEGDGGEASHGLEDSNMSYGSYEEPDPKSNTQDTSFSSIGGYEVSEEEEDEEEEQRSGPSVLSQVHLSEDEEDSEDFHSIAGDSDLDSDE

Target ORF information:

RefSeq Version	XM_005641475.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa (TAF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005641475.2

ORF Insert Sequence:

ATGGGACCTG GCTGGGATTT GCTGCTGCCT GTAGCCGCCG GAGACACTAC CAGCGCCATC 
ATGTCAGACA CGGACAGCGA TGAAGATTCT GCTGGAGGCG GCCCATTTTC TCTAACCGGT 
TTCCTTTTTG GCAACATCAA TGGAGCCGGG CAGCTGGAGG GGGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGTC TGGGCAGCCT GATCACTGAA 
CTCACGGCAA ACGAAGAATT GGCTGGGACC GATGGTGCCC TGGTAAATGA TGAAGGATGG 
ATCAGGAGTA CAGAAGATGC TGTGGACTAT TCAGACATCA CTGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ATCAGCAGAC AATGGGGAGC TTGCAGCCCC TTTGCCACTC AGATTATGAT 
GAAGATGACT ATGATGCCGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAATGAA GAAAGATAAG GACCAGGATG GTCTTACCGG TGAGAAAGTG 
GACTTCAGTA GTTCCTCTGA CTCAGAATCT GAGATGGGAC CTCAGGAAGC ATCACAGGCA 
GAATCTGAGG ATGGAAAGCT GACCCTACCA TTGGCTGGGA TTATGCAACA CGATGCCACC 
AAGCTGTTGC CAAGTGTCAC AGAACTTTTC CCGGAATTTC GGCCTGGGAA GGTATTACGC 
TTCCTTCGTC TTTTCGGACC AGGGAAGAAT GTCCCATCTG TTTGGCGGAG TGCTCGGAGA 
AAGAGGAAGA AGAAGCACCG TGAGCTGATA CAGGAAGAAC AGATCCAGGA GGTGGAGTGC 
TCAGTAGAGT CCGAAGTCAA CAAGAAGTCT TTATGGAATT ATGACTATGC TCCACCACCA 
CCTCCAGAGC AGTGTCTCTC TGATGATGAA ATTACAATGA TGGCTCCTGT TGAATCAAAG 
TTTTCCCAGT CAACTGGAGA TATAGATAAA GTGACAGATA CCAAACCAAG AGTGGCTGAG 
TGGCGTTATG GGCCTGCTCG ACTGTGGTAT GATATGCTGG GTGTCCCTGA AGATGGCAGT 
GGGTTTGATT ATGGCTTCAA ACTGAGAAAA ATGGACCATG AGCCTGTGAT AAAATGTAGA 
ATGATGGAGG ACTTGAGGAA ATTTGAGGAA AACAATGGCT CTGACCTACT GGCTGATGAA 
AACTTCCTGA TGGTGACACA GCTGCACTGG GAGGATGATA TCATCTGGGA TGGGGAGGAT 
GTCAAGCACA AAGGAACAAA GCCTCAGCGT GCAAGCCTGG CAGGCTGGCT TCCTTCCAGC 
ATGACTAGGA ATGCCATGGC CTACAATGTT CAGCAAGGTT TTGCAGCCAC CCTGGATGAT 
GACAAACCTT GGTACTCCAT CTTTCCCATT GACAACGAGG AGCTGGTATA TGGACGCTGG 
GAGGACAATA TCATTTGGGA TGCTCAGGCC ATGCCCAGGC TGTTGGAGCC TCCTGTTTTG 
ACACTTGATC CCAATGATGA GAACCTTATT TTGGAAATTC CTGATGAAAA GGAAGAGGCC 
ACTTCTAACT CCCCCTCCAA GGAAAATAAG AAAGAATCAT CTCTGAAGAA GAGCCGAATT 
CTCTTAGGGA AAACAGGGGT CATCAAGGAG GAACCACAGC AGAACATGTC TCAGCCAGAA 
GTGAAAGATC CATGGAATCT CTCCAATGAT GAATATTACT ACCCCAAGCA ACAGGGTCTT 
CGAGGCACCT TTGGAGGAAA TATTATCCAG CACTCAATTC CTGCAGTGGA ATTACGACAG 
CCCTTCTTTC CTACCCATAT GGGGCCCATC AAACTTCGGC AGTTCCATCG TCCACCCCTG 
AAGAAATATT CCTTCGGGGC ACTCTCTCAG CCAGGTCCCC ACTCAGTCCA GCCCTTGCTG 
AAGCACATCA AAAAGAAGGC TAAGATGAGA GAACAAGAGA GACAAGCTTC TGGTGGTGGA 
GAGATGTTTT TTATGCGCAC ACCTCAGGAC CTCACAGGCA AAGATGGAGA TCTTATTCTT 
GCAGAATACA GTGAGGAAAA TGGACCATTA ATGATGCAGG TTGGCATGGC AACCAAGATA 
AAAAACTATT ATAAGCGGAA ACCTGGAAAA GATCCTGGGG CCCCAGATTG TAAATATGGA 
GAAACTGTTT ACTGTCATAC ATCTCCTTTC CTAGGCTCTC TTCATCCTGG TCAATTACTG 
CAGGCATTTG AGAACAATCT CTTTCGTGCC CCAATTTATC TTCATAAGAT GCCAGAAACT 
GACTTCCTTA TTATTCGGAC AAGACAAGGT TACTACATTC GGGAGTTAGT GGATATTTTT 
GTGGTCGGCC AGCAATGCCC CTTGTTTGAA GTTCCTGGGC CCAACTCCAA AAGAGCTAAT 
ACACATATTC GAGACTTTCT CCAGGTTTTT ATTTACCGCC TCTTCTGGAA GAGTAAAGAT 
CGGCCAAGGC GGATCCGAAT GGAAGATATA AAAAAAGCCT TTCCTTCTCA CTCAGAAAGT 
AGCATCCGCA AGAGGCTAAA GCTCTGCGCT GACTTCAAAC GCACAGGGAT GGACTCAAAC 
TGGTGGGTGC TAAAGTCAGA TTTTCGTTTA CCAACTGAAG AGGAGATCAG AGCTATGGTG 
TCTCCAGAGC AGTGCTGTGC TTATTATAGC ATGATAGCTG CAGAGCAGCG ACTAAAGGAT 
GCTGGCTATG GTGAGAAGTC CTTTTTTGCT CCAGAAGAAG AAAATGAGGA AGATTTCCAG 
ATGAAGATTG ATGATGAAGT TCGCACTGCT CCTTGGAATA CCACAAGGGC TTTCATTGCT 
GCCATGAAGG GCAAGTGTCT CTTGGAGGTG ACTGGGGTGG CAGATCCCAC AGGGTGTGGT 
GAAGGATTCT CCTATGTCAA GATTCCAAAC AAACCAACAC AGCAGAAGGA TGATAAGGAG 
CCTCAGCCAG TGAAGAAGAC AGTGACAGGA ACAGATGCAG ATCTCCGTCG CCTCTCACTG 
AAAAATGCCA AGCAGCTCCT ACGTAAATTT GGTGTGCCTG AGGAAGAGAT TAAAAAGCTG 
TCCCGCTGGG AAGTGATTGA TGTGGTACGC ACAATGTCAA CAGAGCAAGC CCGCTCTGGA 
GAGGGGCCCA TGAGTAAATT TGCCCGTGGA TCAAGGTTTT CTGTGGCTGA GCATCAAGAG 
CGTTACAAAG AGGAATGTCA GCGCATCTTT GATCTACAGA ACAAGGTTTT GTCATCAACT 
GAAATATTGT CAACTGACAC AGACAGCAGC TCAGCCGAAG ACAGTGACTT TGAAGAAATG 
GGAAAGAACA TTGAGAACAT GTTGCAGAAT AAGAAAACCA GCTCTCAGCT GTCACGTGAA 
CGAGAGGAGC AGGAGCGGAA GGAACTGCAG CGGATGCTAC TGGCAGCAGG TTCTGCAGCA 
TCAGGAAACA ATCACAGAGA TGATGATACA GCTTCTGTGA CCAGCCTTAA CTCTTCTGCC 
ACTGGCCGTT GTCTCAAAAT TTATCGCACA TTTCGAGATG AAGAAGGGAA AGAGTATGTT 
CGCTGTGAGA CAGTCCGAAA GCCTGCTGTC ATTGATGCCT ATGTGCGCAT ACGGACCACA 
AAAGATGAGG AATTCATTCG AAAGTTTGCC CTTTTTGATG AACAGCATCG AGAAGAGATG 
CGAAAGGAAC GGCGGAGGAT TCAAGAGCAA CTTAGGCGGC TCAAGCGGAA TCAGGAAAAG 
GAGAAGCTTA AGGGTCCCCC TGAGAAGAAG CCTAAAAAAA TGAAGGAGCG TCCTGATCTA 
AAACTGAAAT GTGGAGCTTG TGGTGCCATT GGGCACATGA GGACAAACAA GTTCTGCCCC 
CTCTACTATC AAACAAATGC TCCACCTTCC AACCCTGTTG CCATGACAGA GGAGCAGGAG 
GAGGAGTTGG AAAAGACAGT CATTCATAAT GATAATGAAG AACTTATCAA GGTTGAAGGG 
ACCAAGATCG TCCTGGGAAA ACAGCTAATT GAGAGTGCAG ATGAGGTTCG AAGAAAATCT 
CTGGTTCTCA AGTTCCCTAA ACAGCAACTT CCTCCCAAGA AGAAACGGCG AGTTGGAACC 
ACTGTTCACT GTGACTACTT AAATAGACCT CATAAATCCA TCCACCGGCG CCGGACAGAC 
CCAATGGTGA CATTGTCCTC CATCTTGGAG TCTATCATCA ATGACATGAG AGATCTTCCA 
AATACCTACC CTTTCCACAC TCCAGTCAAT GCAAAGGTTG TAAAGGACTA CTACAAAATC 
ATCACTCGAC CAATGGACTT ACAAACACTC CGTGAAAATG TGCGCAAACG CCTTTACCCA 
TCTCGGGAAG AGTTCAGAGA ACATTTGGAG CTCATTGTGA AAAATAGTGC AACCTATAAT 
GGTCCGAAGC ACTCATTGAC CCAGATCTCT CAATCTATGC TGGATCTCTG TGATGAAAAA 
CTCAAAGAGA AAGAAGATAA ATTGGCTCGT TTAGAGAAAG CTATCAACCC CTTGCTGGAT 
GATGATGACC AAGTAGCATT TTCTTTCATT CTGGACAACA TTGTCACCCA GAAAATGATG 
GCAGTTCCAG ATTCTTGGCC ATTTCATCAC CCAGTTAATA AGAAGTTTGT TCCAGATTAT 
TACAAAGTGA TTATCAGTCC AATGGATTTA GAGACTATAC GTAAGAATAT CTCCAAGCAC 
AAGTACCAGA GTCGGGAGAG CTTTCTGGAT GATGTCAACC TTATTCTGGC CAACAGTGTT 
AAGTATAATG GGCCTGAAAG TCAGTATACT AAGACTGCTC AGGAGATTGT AAATGTCTGT 
TACCAGACAT TGACTGAGTA TGATGAACAT TTGACTCAAC TTGAGAAGGA TATTTGTACA 
GCTAAGGAAG CGGCTTTGGA GGAAGCAGAA TTAGAAAGCT TGGACCCAAT GACCCCAGGG 
CCTTATACAC CTCAGGCTAA GCCTCCTGAT TTGTATGACA CCAACACATC CCTCAGTATG 
TCCCGAGATG CCTCTGTATT TCAAGATGAG AGCAATATGT CTGTCCTGGA TATTCCCACT 
GCCACTCCAG AAAAGCAGGT GACACAGATG CGCCAGGGCC GAGGTAGGCT GGGCGAGGAA 
GACTCTGATG TAGATATTGA AGGGTATGAT GATGAGGAGG AGGATGGGAA ACCTAAGACT 
CCAGCCCCAG AAGGTGAAGA TGCAGATGGT GATCTTGCAG ATGAAGAGGA AGGAAGTATG 
CAACAGCCTC AAGCCAGTGT CCTGTATGAG GATTTGCTTA TGTCTGAAGG AGAAGATGAT 
GAAGAAGATG CTGGGAGTGA TGAAGAAGGA GACAATCCTT TCTCTGCTAT CCAACTGAGT 
GAAAGTGGAA GTGACTCTGA TGTTGGATCT AGTGGGATAA GACCCAAACA GCCCCGCATG 
CTTCAGGAAA ACACAAGGAT GGGCATGGAA AATGAAGAAA GCATGATGTC CTATGAGGGA 
GATGGTGGGG AGGCTTCTCA TGGTTTGGAG GATAGCAATA TGAGTTATGG GAGCTATGAG 
GAGCCTGATC CCAAGTCGAA CACCCAAGAT ACAAGCTTCA GCAGCATCGG TGGGTATGAG 
GTATCAGAGG AAGAAGAAGA TGAGGAGGAA GAGCAGCGCT CTGGGCCAAG TGTACTAAGC 
CAGGTCCACC TGTCAGAGGA TGAGGAGGAC AGTGAGGATT TCCACTCCAT TGCTGGGGAC 
AGTGATTTGG ACTCTGATGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAF1 ( XM_005641475.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005641532.1 Canis lupus familiaris TATA-box binding protein associated factor 1 (TAF1), transcript variant X4, mRNA.

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CloneID	OCe32529
Clone ID Related Accession (Same CDS sequence)	XM_005641475.2 , XM_005641475.3
Accession Version	XM_005641475.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5724bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	transcription initiation factor TFIID subunit 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005641475.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACCTG GCTGGGATTT GCTGCTGCCT GTAGCCGCCG GAGACACTAC CAGCGCCATC 
ATGTCAGACA CGGACAGCGA TGAAGATTCT GCTGGAGGCG GCCCATTTTC TCTAACCGGT 
TTCCTTTTTG GCAACATCAA TGGAGCCGGG CAGCTGGAGG GGGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGTC TGGGCAGCCT GATCACTGAA 
CTCACGGCAA ACGAAGAATT GGCTGGGACC GATGGTGCCC TGGTAAATGA TGAAGGATGG 
ATCAGGAGTA CAGAAGATGC TGTGGACTAT TCAGACATCA CTGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ATCAGCAGAC AATGGGGAGC TTGCAGCCCC TTTGCCACTC AGATTATGAT 
GAAGATGACT ATGATGCCGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAATGAA GAAAGATAAG GACCAGGATG GTCTTACCGG TGAGAAAGTG 
GACTTCAGTA GTTCCTCTGA CTCAGAATCT GAGATGGGAC CTCAGGAAGC ATCACAGGCA 
GAATCTGAGG ATGGAAAGCT GACCCTACCA TTGGCTGGGA TTATGCAACA CGATGCCACC 
AAGCTGTTGC CAAGTGTCAC AGAACTTTTC CCGGAATTTC GGCCTGGGAA GGTATTACGC 
TTCCTTCGTC TTTTCGGACC AGGGAAGAAT GTCCCATCTG TTTGGCGGAG TGCTCGGAGA 
AAGAGGAAGA AGAAGCACCG TGAGCTGATA CAGGAAGAAC AGATCCAGGA GGTGGAGTGC 
TCAGTAGAGT CCGAAGTCAA CAAGAAGTCT TTATGGAATT ATGACTATGC TCCACCACCA 
CCTCCAGAGC AGTGTCTCTC TGATGATGAA ATTACAATGA TGGCTCCTGT TGAATCAAAG 
TTTTCCCAGT CAACTGGAGA TATAGATAAA GTGACAGATA CCAAACCAAG AGTGGCTGAG 
TGGCGTTATG GGCCTGCTCG ACTGTGGTAT GATATGCTGG GTGTCCCTGA AGATGGCAGT 
GGGTTTGATT ATGGCTTCAA ACTGAGAAAA ATGGACCATG AGCCTGTGAT AAAATGTAGA 
ATGATGGAGG ACTTGAGGAA ATTTGAGGAA AACAATGGCT CTGACCTACT GGCTGATGAA 
AACTTCCTGA TGGTGACACA GCTGCACTGG GAGGATGATA TCATCTGGGA TGGGGAGGAT 
GTCAAGCACA AAGGAACAAA GCCTCAGCGT GCAAGCCTGG CAGGCTGGCT TCCTTCCAGC 
ATGACTAGGA ATGCCATGGC CTACAATGTT CAGCAAGGTT TTGCAGCCAC CCTGGATGAT 
GACAAACCTT GGTACTCCAT CTTTCCCATT GACAACGAGG AGCTGGTATA TGGACGCTGG 
GAGGACAATA TCATTTGGGA TGCTCAGGCC ATGCCCAGGC TGTTGGAGCC TCCTGTTTTG 
ACACTTGATC CCAATGATGA GAACCTTATT TTGGAAATTC CTGATGAAAA GGAAGAGGCC 
ACTTCTAACT CCCCCTCCAA GGAAAATAAG AAAGAATCAT CTCTGAAGAA GAGCCGAATT 
CTCTTAGGGA AAACAGGGGT CATCAAGGAG GAACCACAGC AGAACATGTC TCAGCCAGAA 
GTGAAAGATC CATGGAATCT CTCCAATGAT GAATATTACT ACCCCAAGCA ACAGGGTCTT 
CGAGGCACCT TTGGAGGAAA TATTATCCAG CACTCAATTC CTGCAGTGGA ATTACGACAG 
CCCTTCTTTC CTACCCATAT GGGGCCCATC AAACTTCGGC AGTTCCATCG TCCACCCCTG 
AAGAAATATT CCTTCGGGGC ACTCTCTCAG CCAGGTCCCC ACTCAGTCCA GCCCTTGCTG 
AAGCACATCA AAAAGAAGGC TAAGATGAGA GAACAAGAGA GACAAGCTTC TGGTGGTGGA 
GAGATGTTTT TTATGCGCAC ACCTCAGGAC CTCACAGGCA AAGATGGAGA TCTTATTCTT 
GCAGAATACA GTGAGGAAAA TGGACCATTA ATGATGCAGG TTGGCATGGC AACCAAGATA 
AAAAACTATT ATAAGCGGAA ACCTGGAAAA GATCCTGGGG CCCCAGATTG TAAATATGGA 
GAAACTGTTT ACTGTCATAC ATCTCCTTTC CTAGGCTCTC TTCATCCTGG TCAATTACTG 
CAGGCATTTG AGAACAATCT CTTTCGTGCC CCAATTTATC TTCATAAGAT GCCAGAAACT 
GACTTCCTTA TTATTCGGAC AAGACAAGGT TACTACATTC GGGAGTTAGT GGATATTTTT 
GTGGTCGGCC AGCAATGCCC CTTGTTTGAA GTTCCTGGGC CCAACTCCAA AAGAGCTAAT 
ACACATATTC GAGACTTTCT CCAGGTTTTT ATTTACCGCC TCTTCTGGAA GAGTAAAGAT 
CGGCCAAGGC GGATCCGAAT GGAAGATATA AAAAAAGCCT TTCCTTCTCA CTCAGAAAGT 
AGCATCCGCA AGAGGCTAAA GCTCTGCGCT GACTTCAAAC GCACAGGGAT GGACTCAAAC 
TGGTGGGTGC TAAAGTCAGA TTTTCGTTTA CCAACTGAAG AGGAGATCAG AGCTATGGTG 
TCTCCAGAGC AGTGCTGTGC TTATTATAGC ATGATAGCTG CAGAGCAGCG ACTAAAGGAT 
GCTGGCTATG GTGAGAAGTC CTTTTTTGCT CCAGAAGAAG AAAATGAGGA AGATTTCCAG 
ATGAAGATTG ATGATGAAGT TCGCACTGCT CCTTGGAATA CCACAAGGGC TTTCATTGCT 
GCCATGAAGG GCAAGTGTCT CTTGGAGGTG ACTGGGGTGG CAGATCCCAC AGGGTGTGGT 
GAAGGATTCT CCTATGTCAA GATTCCAAAC AAACCAACAC AGCAGAAGGA TGATAAGGAG 
CCTCAGCCAG TGAAGAAGAC AGTGACAGGA ACAGATGCAG ATCTCCGTCG CCTCTCACTG 
AAAAATGCCA AGCAGCTCCT ACGTAAATTT GGTGTGCCTG AGGAAGAGAT TAAAAAGCTG 
TCCCGCTGGG AAGTGATTGA TGTGGTACGC ACAATGTCAA CAGAGCAAGC CCGCTCTGGA 
GAGGGGCCCA TGAGTAAATT TGCCCGTGGA TCAAGGTTTT CTGTGGCTGA GCATCAAGAG 
CGTTACAAAG AGGAATGTCA GCGCATCTTT GATCTACAGA ACAAGGTTTT GTCATCAACT 
GAAATATTGT CAACTGACAC AGACAGCAGC TCAGCCGAAG ACAGTGACTT TGAAGAAATG 
GGAAAGAACA TTGAGAACAT GTTGCAGAAT AAGAAAACCA GCTCTCAGCT GTCACGTGAA 
CGAGAGGAGC AGGAGCGGAA GGAACTGCAG CGGATGCTAC TGGCAGCAGG TTCTGCAGCA 
TCAGGAAACA ATCACAGAGA TGATGATACA GCTTCTGTGA CCAGCCTTAA CTCTTCTGCC 
ACTGGCCGTT GTCTCAAAAT TTATCGCACA TTTCGAGATG AAGAAGGGAA AGAGTATGTT 
CGCTGTGAGA CAGTCCGAAA GCCTGCTGTC ATTGATGCCT ATGTGCGCAT ACGGACCACA 
AAAGATGAGG AATTCATTCG AAAGTTTGCC CTTTTTGATG AACAGCATCG AGAAGAGATG 
CGAAAGGAAC GGCGGAGGAT TCAAGAGCAA CTTAGGCGGC TCAAGCGGAA TCAGGAAAAG 
GAGAAGCTTA AGGGTCCCCC TGAGAAGAAG CCTAAAAAAA TGAAGGAGCG TCCTGATCTA 
AAACTGAAAT GTGGAGCTTG TGGTGCCATT GGGCACATGA GGACAAACAA GTTCTGCCCC 
CTCTACTATC AAACAAATGC TCCACCTTCC AACCCTGTTG CCATGACAGA GGAGCAGGAG 
GAGGAGTTGG AAAAGACAGT CATTCATAAT GATAATGAAG AACTTATCAA GGTTGAAGGG 
ACCAAGATCG TCCTGGGAAA ACAGCTAATT GAGAGTGCAG ATGAGGTTCG AAGAAAATCT 
CTGGTTCTCA AGTTCCCTAA ACAGCAACTT CCTCCCAAGA AGAAACGGCG AGTTGGAACC 
ACTGTTCACT GTGACTACTT AAATAGACCT CATAAATCCA TCCACCGGCG CCGGACAGAC 
CCAATGGTGA CATTGTCCTC CATCTTGGAG TCTATCATCA ATGACATGAG AGATCTTCCA 
AATACCTACC CTTTCCACAC TCCAGTCAAT GCAAAGGTTG TAAAGGACTA CTACAAAATC 
ATCACTCGAC CAATGGACTT ACAAACACTC CGTGAAAATG TGCGCAAACG CCTTTACCCA 
TCTCGGGAAG AGTTCAGAGA ACATTTGGAG CTCATTGTGA AAAATAGTGC AACCTATAAT 
GGTCCGAAGC ACTCATTGAC CCAGATCTCT CAATCTATGC TGGATCTCTG TGATGAAAAA 
CTCAAAGAGA AAGAAGATAA ATTGGCTCGT TTAGAGAAAG CTATCAACCC CTTGCTGGAT 
GATGATGACC AAGTAGCATT TTCTTTCATT CTGGACAACA TTGTCACCCA GAAAATGATG 
GCAGTTCCAG ATTCTTGGCC ATTTCATCAC CCAGTTAATA AGAAGTTTGT TCCAGATTAT 
TACAAAGTGA TTATCAGTCC AATGGATTTA GAGACTATAC GTAAGAATAT CTCCAAGCAC 
AAGTACCAGA GTCGGGAGAG CTTTCTGGAT GATGTCAACC TTATTCTGGC CAACAGTGTT 
AAGTATAATG GGCCTGAAAG TCAGTATACT AAGACTGCTC AGGAGATTGT AAATGTCTGT 
TACCAGACAT TGACTGAGTA TGATGAACAT TTGACTCAAC TTGAGAAGGA TATTTGTACA 
GCTAAGGAAG CGGCTTTGGA GGAAGCAGAA TTAGAAAGCT TGGACCCAAT GACCCCAGGG 
CCTTATACAC CTCAGGCTAA GCCTCCTGAT TTGTATGACA CCAACACATC CCTCAGTATG 
TCCCGAGATG CCTCTGTATT TCAAGATGAG AGCAATATGT CTGTCCTGGA TATTCCCACT 
GCCACTCCAG AAAAGCAGGT GACACAGATG CGCCAGGGCC GAGGTAGGCT GGGCGAGGAA 
GACTCTGATG TAGATATTGA AGGGTATGAT GATGAGGAGG AGGATGGGAA ACCTAAGACT 
CCAGCCCCAG AAGGTGAAGA TGCAGATGGT GATCTTGCAG ATGAAGAGGA AGGAAGTATG 
CAACAGCCTC AAGCCAGTGT CCTGTATGAG GATTTGCTTA TGTCTGAAGG AGAAGATGAT 
GAAGAAGATG CTGGGAGTGA TGAAGAAGGA GACAATCCTT TCTCTGCTAT CCAACTGAGT 
GAAAGTGGAA GTGACTCTGA TGTTGGATCT AGTGGGATAA GACCCAAACA GCCCCGCATG 
CTTCAGGAAA ACACAAGGAT GGGCATGGAA AATGAAGAAA GCATGATGTC CTATGAGGGA 
GATGGTGGGG AGGCTTCTCA TGGTTTGGAG GATAGCAATA TGAGTTATGG GAGCTATGAG 
GAGCCTGATC CCAAGTCGAA CACCCAAGAT ACAAGCTTCA GCAGCATCGG TGGGTATGAG 
GTATCAGAGG AAGAAGAAGA TGAGGAGGAA GAGCAGCGCT CTGGGCCAAG TGTACTAAGC 
CAGGTCCACC TGTCAGAGGA TGAGGAGGAC AGTGAGGATT TCCACTCCAT TGCTGGGGAC 
AGTGATTTGG ACTCTGATGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005641532.1
CDS	104..5827
Translation	MGPGWDLLLPVAAGDTTSAIMSDTDSDEDSAGGGPFSLTGFLFGNINGAGQLEGESVLDDECKKHLAGLGALGLGSLITELTANEELAGTDGALVNDEGWIRSTEDAVDYSDITEVAEDESRRYQQTMGSLQPLCHSDYDEDDYDADCEDIDCKLMPPPPPPPGPMKKDKDQDGLTGEKVDFSSSSDSESEMGPQEASQAESEDGKLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSARRKRKKKHRELIQEEQIQEVECSVESEVNKKSLWNYDYAPPPPPEQCLSDDEITMMAPVESKFSQSTGDIDKVTDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKLRKMDHEPVIKCRMMEDLRKFEENNGSDLLADENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDKPWYSIFPIDNEELVYGRWEDNIIWDAQAMPRLLEPPVLTLDPNDENLILEIPDEKEEATSNSPSKENKKESSLKKSRILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYYIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYYSMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEILSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMLLAAGSAASGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKPAVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIISPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNGPESQYTKTAQEIVNVCYQTLTEYDEHLTQLEKDICTAKEAALEEAELESLDPMTPGPYTPQAKPPDLYDTNTSLSMSRDASVFQDESNMSVLDIPTATPEKQVTQMRQGRGRLGEEDSDVDIEGYDDEEEDGKPKTPAPEGEDADGDLADEEEGSMQQPQASVLYEDLLMSEGEDDEEDAGSDEEGDNPFSAIQLSESGSDSDVGSSGIRPKQPRMLQENTRMGMENEESMMSYEGDGGEASHGLEDSNMSYGSYEEPDPKSNTQDTSFSSIGGYEVSEEEEDEEEEQRSGPSVLSQVHLSEDEEDSEDFHSIAGDSDLDSDE

Target ORF information:

RefSeq Version	XM_005641475.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris TATA-box binding protein associated factor 1 (TAF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005641475.3

ORF Insert Sequence:

ATGGGACCTG GCTGGGATTT GCTGCTGCCT GTAGCCGCCG GAGACACTAC CAGCGCCATC 
ATGTCAGACA CGGACAGCGA TGAAGATTCT GCTGGAGGCG GCCCATTTTC TCTAACCGGT 
TTCCTTTTTG GCAACATCAA TGGAGCCGGG CAGCTGGAGG GGGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGTC TGGGCAGCCT GATCACTGAA 
CTCACGGCAA ACGAAGAATT GGCTGGGACC GATGGTGCCC TGGTAAATGA TGAAGGATGG 
ATCAGGAGTA CAGAAGATGC TGTGGACTAT TCAGACATCA CTGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ATCAGCAGAC AATGGGGAGC TTGCAGCCCC TTTGCCACTC AGATTATGAT 
GAAGATGACT ATGATGCCGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAATGAA GAAAGATAAG GACCAGGATG GTCTTACCGG TGAGAAAGTG 
GACTTCAGTA GTTCCTCTGA CTCAGAATCT GAGATGGGAC CTCAGGAAGC ATCACAGGCA 
GAATCTGAGG ATGGAAAGCT GACCCTACCA TTGGCTGGGA TTATGCAACA CGATGCCACC 
AAGCTGTTGC CAAGTGTCAC AGAACTTTTC CCGGAATTTC GGCCTGGGAA GGTATTACGC 
TTCCTTCGTC TTTTCGGACC AGGGAAGAAT GTCCCATCTG TTTGGCGGAG TGCTCGGAGA 
AAGAGGAAGA AGAAGCACCG TGAGCTGATA CAGGAAGAAC AGATCCAGGA GGTGGAGTGC 
TCAGTAGAGT CCGAAGTCAA CAAGAAGTCT TTATGGAATT ATGACTATGC TCCACCACCA 
CCTCCAGAGC AGTGTCTCTC TGATGATGAA ATTACAATGA TGGCTCCTGT TGAATCAAAG 
TTTTCCCAGT CAACTGGAGA TATAGATAAA GTGACAGATA CCAAACCAAG AGTGGCTGAG 
TGGCGTTATG GGCCTGCTCG ACTGTGGTAT GATATGCTGG GTGTCCCTGA AGATGGCAGT 
GGGTTTGATT ATGGCTTCAA ACTGAGAAAA ATGGACCATG AGCCTGTGAT AAAATGTAGA 
ATGATGGAGG ACTTGAGGAA ATTTGAGGAA AACAATGGCT CTGACCTACT GGCTGATGAA 
AACTTCCTGA TGGTGACACA GCTGCACTGG GAGGATGATA TCATCTGGGA TGGGGAGGAT 
GTCAAGCACA AAGGAACAAA GCCTCAGCGT GCAAGCCTGG CAGGCTGGCT TCCTTCCAGC 
ATGACTAGGA ATGCCATGGC CTACAATGTT CAGCAAGGTT TTGCAGCCAC CCTGGATGAT 
GACAAACCTT GGTACTCCAT CTTTCCCATT GACAACGAGG AGCTGGTATA TGGACGCTGG 
GAGGACAATA TCATTTGGGA TGCTCAGGCC ATGCCCAGGC TGTTGGAGCC TCCTGTTTTG 
ACACTTGATC CCAATGATGA GAACCTTATT TTGGAAATTC CTGATGAAAA GGAAGAGGCC 
ACTTCTAACT CCCCCTCCAA GGAAAATAAG AAAGAATCAT CTCTGAAGAA GAGCCGAATT 
CTCTTAGGGA AAACAGGGGT CATCAAGGAG GAACCACAGC AGAACATGTC TCAGCCAGAA 
GTGAAAGATC CATGGAATCT CTCCAATGAT GAATATTACT ACCCCAAGCA ACAGGGTCTT 
CGAGGCACCT TTGGAGGAAA TATTATCCAG CACTCAATTC CTGCAGTGGA ATTACGACAG 
CCCTTCTTTC CTACCCATAT GGGGCCCATC AAACTTCGGC AGTTCCATCG TCCACCCCTG 
AAGAAATATT CCTTCGGGGC ACTCTCTCAG CCAGGTCCCC ACTCAGTCCA GCCCTTGCTG 
AAGCACATCA AAAAGAAGGC TAAGATGAGA GAACAAGAGA GACAAGCTTC TGGTGGTGGA 
GAGATGTTTT TTATGCGCAC ACCTCAGGAC CTCACAGGCA AAGATGGAGA TCTTATTCTT 
GCAGAATACA GTGAGGAAAA TGGACCATTA ATGATGCAGG TTGGCATGGC AACCAAGATA 
AAAAACTATT ATAAGCGGAA ACCTGGAAAA GATCCTGGGG CCCCAGATTG TAAATATGGA 
GAAACTGTTT ACTGTCATAC ATCTCCTTTC CTAGGCTCTC TTCATCCTGG TCAATTACTG 
CAGGCATTTG AGAACAATCT CTTTCGTGCC CCAATTTATC TTCATAAGAT GCCAGAAACT 
GACTTCCTTA TTATTCGGAC AAGACAAGGT TACTACATTC GGGAGTTAGT GGATATTTTT 
GTGGTCGGCC AGCAATGCCC CTTGTTTGAA GTTCCTGGGC CCAACTCCAA AAGAGCTAAT 
ACACATATTC GAGACTTTCT CCAGGTTTTT ATTTACCGCC TCTTCTGGAA GAGTAAAGAT 
CGGCCAAGGC GGATCCGAAT GGAAGATATA AAAAAAGCCT TTCCTTCTCA CTCAGAAAGT 
AGCATCCGCA AGAGGCTAAA GCTCTGCGCT GACTTCAAAC GCACAGGGAT GGACTCAAAC 
TGGTGGGTGC TAAAGTCAGA TTTTCGTTTA CCAACTGAAG AGGAGATCAG AGCTATGGTG 
TCTCCAGAGC AGTGCTGTGC TTATTATAGC ATGATAGCTG CAGAGCAGCG ACTAAAGGAT 
GCTGGCTATG GTGAGAAGTC CTTTTTTGCT CCAGAAGAAG AAAATGAGGA AGATTTCCAG 
ATGAAGATTG ATGATGAAGT TCGCACTGCT CCTTGGAATA CCACAAGGGC TTTCATTGCT 
GCCATGAAGG GCAAGTGTCT CTTGGAGGTG ACTGGGGTGG CAGATCCCAC AGGGTGTGGT 
GAAGGATTCT CCTATGTCAA GATTCCAAAC AAACCAACAC AGCAGAAGGA TGATAAGGAG 
CCTCAGCCAG TGAAGAAGAC AGTGACAGGA ACAGATGCAG ATCTCCGTCG CCTCTCACTG 
AAAAATGCCA AGCAGCTCCT ACGTAAATTT GGTGTGCCTG AGGAAGAGAT TAAAAAGCTG 
TCCCGCTGGG AAGTGATTGA TGTGGTACGC ACAATGTCAA CAGAGCAAGC CCGCTCTGGA 
GAGGGGCCCA TGAGTAAATT TGCCCGTGGA TCAAGGTTTT CTGTGGCTGA GCATCAAGAG 
CGTTACAAAG AGGAATGTCA GCGCATCTTT GATCTACAGA ACAAGGTTTT GTCATCAACT 
GAAATATTGT CAACTGACAC AGACAGCAGC TCAGCCGAAG ACAGTGACTT TGAAGAAATG 
GGAAAGAACA TTGAGAACAT GTTGCAGAAT AAGAAAACCA GCTCTCAGCT GTCACGTGAA 
CGAGAGGAGC AGGAGCGGAA GGAACTGCAG CGGATGCTAC TGGCAGCAGG TTCTGCAGCA 
TCAGGAAACA ATCACAGAGA TGATGATACA GCTTCTGTGA CCAGCCTTAA CTCTTCTGCC 
ACTGGCCGTT GTCTCAAAAT TTATCGCACA TTTCGAGATG AAGAAGGGAA AGAGTATGTT 
CGCTGTGAGA CAGTCCGAAA GCCTGCTGTC ATTGATGCCT ATGTGCGCAT ACGGACCACA 
AAAGATGAGG AATTCATTCG AAAGTTTGCC CTTTTTGATG AACAGCATCG AGAAGAGATG 
CGAAAGGAAC GGCGGAGGAT TCAAGAGCAA CTTAGGCGGC TCAAGCGGAA TCAGGAAAAG 
GAGAAGCTTA AGGGTCCCCC TGAGAAGAAG CCTAAAAAAA TGAAGGAGCG TCCTGATCTA 
AAACTGAAAT GTGGAGCTTG TGGTGCCATT GGGCACATGA GGACAAACAA GTTCTGCCCC 
CTCTACTATC AAACAAATGC TCCACCTTCC AACCCTGTTG CCATGACAGA GGAGCAGGAG 
GAGGAGTTGG AAAAGACAGT CATTCATAAT GATAATGAAG AACTTATCAA GGTTGAAGGG 
ACCAAGATCG TCCTGGGAAA ACAGCTAATT GAGAGTGCAG ATGAGGTTCG AAGAAAATCT 
CTGGTTCTCA AGTTCCCTAA ACAGCAACTT CCTCCCAAGA AGAAACGGCG AGTTGGAACC 
ACTGTTCACT GTGACTACTT AAATAGACCT CATAAATCCA TCCACCGGCG CCGGACAGAC 
CCAATGGTGA CATTGTCCTC CATCTTGGAG TCTATCATCA ATGACATGAG AGATCTTCCA 
AATACCTACC CTTTCCACAC TCCAGTCAAT GCAAAGGTTG TAAAGGACTA CTACAAAATC 
ATCACTCGAC CAATGGACTT ACAAACACTC CGTGAAAATG TGCGCAAACG CCTTTACCCA 
TCTCGGGAAG AGTTCAGAGA ACATTTGGAG CTCATTGTGA AAAATAGTGC AACCTATAAT 
GGTCCGAAGC ACTCATTGAC CCAGATCTCT CAATCTATGC TGGATCTCTG TGATGAAAAA 
CTCAAAGAGA AAGAAGATAA ATTGGCTCGT TTAGAGAAAG CTATCAACCC CTTGCTGGAT 
GATGATGACC AAGTAGCATT TTCTTTCATT CTGGACAACA TTGTCACCCA GAAAATGATG 
GCAGTTCCAG ATTCTTGGCC ATTTCATCAC CCAGTTAATA AGAAGTTTGT TCCAGATTAT 
TACAAAGTGA TTATCAGTCC AATGGATTTA GAGACTATAC GTAAGAATAT CTCCAAGCAC 
AAGTACCAGA GTCGGGAGAG CTTTCTGGAT GATGTCAACC TTATTCTGGC CAACAGTGTT 
AAGTATAATG GGCCTGAAAG TCAGTATACT AAGACTGCTC AGGAGATTGT AAATGTCTGT 
TACCAGACAT TGACTGAGTA TGATGAACAT TTGACTCAAC TTGAGAAGGA TATTTGTACA 
GCTAAGGAAG CGGCTTTGGA GGAAGCAGAA TTAGAAAGCT TGGACCCAAT GACCCCAGGG 
CCTTATACAC CTCAGGCTAA GCCTCCTGAT TTGTATGACA CCAACACATC CCTCAGTATG 
TCCCGAGATG CCTCTGTATT TCAAGATGAG AGCAATATGT CTGTCCTGGA TATTCCCACT 
GCCACTCCAG AAAAGCAGGT GACACAGATG CGCCAGGGCC GAGGTAGGCT GGGCGAGGAA 
GACTCTGATG TAGATATTGA AGGGTATGAT GATGAGGAGG AGGATGGGAA ACCTAAGACT 
CCAGCCCCAG AAGGTGAAGA TGCAGATGGT GATCTTGCAG ATGAAGAGGA AGGAAGTATG 
CAACAGCCTC AAGCCAGTGT CCTGTATGAG GATTTGCTTA TGTCTGAAGG AGAAGATGAT 
GAAGAAGATG CTGGGAGTGA TGAAGAAGGA GACAATCCTT TCTCTGCTAT CCAACTGAGT 
GAAAGTGGAA GTGACTCTGA TGTTGGATCT AGTGGGATAA GACCCAAACA GCCCCGCATG 
CTTCAGGAAA ACACAAGGAT GGGCATGGAA AATGAAGAAA GCATGATGTC CTATGAGGGA 
GATGGTGGGG AGGCTTCTCA TGGTTTGGAG GATAGCAATA TGAGTTATGG GAGCTATGAG 
GAGCCTGATC CCAAGTCGAA CACCCAAGAT ACAAGCTTCA GCAGCATCGG TGGGTATGAG 
GTATCAGAGG AAGAAGAAGA TGAGGAGGAA GAGCAGCGCT CTGGGCCAAG TGTACTAAGC 
CAGGTCCACC TGTCAGAGGA TGAGGAGGAC AGTGAGGATT TCCACTCCAT TGCTGGGGAC 
AGTGATTTGG ACTCTGATGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAF1 ( XM_005641477.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005641534.1 Canis lupus familiaris TATA-box binding protein associated factor 1 (TAF1), transcript variant X6, mRNA.

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CloneID	OCe32531
Clone ID Related Accession (Same CDS sequence)	XM_005641477.3 , XM_005641477.2
Accession Version	XM_005641477.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	transcription initiation factor TFIID subunit 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005641477.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACCTG GCTGGGATTT GCTGCTGCCT GTAGCCGCCG GAGACACTAC CAGCGCCATC 
ATGTCAGACA CGGACAGCGA TGAAGATTCT GCTGGAGGCG GCCCATTTTC TCTAACCGGT 
TTCCTTTTTG GCAACATCAA TGGAGCCGGG CAGCTGGAGG GGGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGTC TGGGCAGCCT GATCACTGAA 
CTCACGGCAA ACGAAGAATT GGCTGGGACC GATGGTGCCC TGGTAAATGA TGAAGGATGG 
ATCAGGAGTA CAGAAGATGC TGTGGACTAT TCAGACATCA CTGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ATCAGCAGAC AATGGGGAGC TTGCAGCCCC TTTGCCACTC AGATTATGAT 
GAAGATGACT ATGATGCCGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAATGAA GAAAGATAAG GACCAGGATG GTCTTACCGG TGTGTCTGAG 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCATCACAG 
GCAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA ACACGATGCC 
ACCAAGCTGT TGCCAAGTGT CACAGAACTT TTCCCGGAAT TTCGGCCTGG GAAGGTATTA 
CGCTTCCTTC GTCTTTTCGG ACCAGGGAAG AATGTCCCAT CTGTTTGGCG GAGTGCTCGG 
AGAAAGAGGA AGAAGAAGCA CCGTGAGCTG ATACAGGAAG AACAGATCCA GGAGGTGGAG 
TGCTCAGTAG AGTCCGAAGT CAACAAGAAG TCTTTATGGA ATTATGACTA TGCTCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATTACAA TGATGGCTCC TGTTGAATCA 
AAGTTTTCCC AGTCAACTGG AGATATAGAT AAAGTGACAG ATACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ATTATGGCTT CAAACTGAGA AAAATGGACC ATGAGCCTGT GATAAAATGT 
AGAATGATGG AGGACTTGAG GAAATTTGAG GAAAACAATG GCTCTGACCT ACTGGCTGAT 
GAAAACTTCC TGATGGTGAC ACAGCTGCAC TGGGAGGATG ATATCATCTG GGATGGGGAG 
GATGTCAAGC ACAAAGGAAC AAAGCCTCAG CGTGCAAGCC TGGCAGGCTG GCTTCCTTCC 
AGCATGACTA GGAATGCCAT GGCCTACAAT GTTCAGCAAG GTTTTGCAGC CACCCTGGAT 
GATGACAAAC CTTGGTACTC CATCTTTCCC ATTGACAACG AGGAGCTGGT ATATGGACGC 
TGGGAGGACA ATATCATTTG GGATGCTCAG GCCATGCCCA GGCTGTTGGA GCCTCCTGTT 
TTGACACTTG ATCCCAATGA TGAGAACCTT ATTTTGGAAA TTCCTGATGA AAAGGAAGAG 
GCCACTTCTA ACTCCCCCTC CAAGGAAAAT AAGAAAGAAT CATCTCTGAA GAAGAGCCGA 
ATTCTCTTAG GGAAAACAGG GGTCATCAAG GAGGAACCAC AGCAGAACAT GTCTCAGCCA 
GAAGTGAAAG ATCCATGGAA TCTCTCCAAT GATGAATATT ACTACCCCAA GCAACAGGGT 
CTTCGAGGCA CCTTTGGAGG AAATATTATC CAGCACTCAA TTCCTGCAGT GGAATTACGA 
CAGCCCTTCT TTCCTACCCA TATGGGGCCC ATCAAACTTC GGCAGTTCCA TCGTCCACCC 
CTGAAGAAAT ATTCCTTCGG GGCACTCTCT CAGCCAGGTC CCCACTCAGT CCAGCCCTTG 
CTGAAGCACA TCAAAAAGAA GGCTAAGATG AGAGAACAAG AGAGACAAGC TTCTGGTGGT 
GGAGAGATGT TTTTTATGCG CACACCTCAG GACCTCACAG GCAAAGATGG AGATCTTATT 
CTTGCAGAAT ACAGTGAGGA AAATGGACCA TTAATGATGC AGGTTGGCAT GGCAACCAAG 
ATAAAAAACT ATTATAAGCG GAAACCTGGA AAAGATCCTG GGGCCCCAGA TTGTAAATAT 
GGAGAAACTG TTTACTGTCA TACATCTCCT TTCCTAGGCT CTCTTCATCC TGGTCAATTA 
CTGCAGGCAT TTGAGAACAA TCTCTTTCGT GCCCCAATTT ATCTTCATAA GATGCCAGAA 
ACTGACTTCC TTATTATTCG GACAAGACAA GGTTACTACA TTCGGGAGTT AGTGGATATT 
TTTGTGGTCG GCCAGCAATG CCCCTTGTTT GAAGTTCCTG GGCCCAACTC CAAAAGAGCT 
AATACACATA TTCGAGACTT TCTCCAGGTT TTTATTTACC GCCTCTTCTG GAAGAGTAAA 
GATCGGCCAA GGCGGATCCG AATGGAAGAT ATAAAAAAAG CCTTTCCTTC TCACTCAGAA 
AGTAGCATCC GCAAGAGGCT AAAGCTCTGC GCTGACTTCA AACGCACAGG GATGGACTCA 
AACTGGTGGG TGCTAAAGTC AGATTTTCGT TTACCAACTG AAGAGGAGAT CAGAGCTATG 
GTGTCTCCAG AGCAGTGCTG TGCTTATTAT AGCATGATAG CTGCAGAGCA GCGACTAAAG 
GATGCTGGCT ATGGTGAGAA GTCCTTTTTT GCTCCAGAAG AAGAAAATGA GGAAGATTTC 
CAGATGAAGA TTGATGATGA AGTTCGCACT GCTCCTTGGA ATACCACAAG GGCTTTCATT 
GCTGCCATGA AGGGCAAGTG TCTCTTGGAG GTGACTGGGG TGGCAGATCC CACAGGGTGT 
GGTGAAGGAT TCTCCTATGT CAAGATTCCA AACAAACCAA CACAGCAGAA GGATGATAAG 
GAGCCTCAGC CAGTGAAGAA GACAGTGACA GGAACAGATG CAGATCTCCG TCGCCTCTCA 
CTGAAAAATG CCAAGCAGCT CCTACGTAAA TTTGGTGTGC CTGAGGAAGA GATTAAAAAG 
CTGTCCCGCT GGGAAGTGAT TGATGTGGTA CGCACAATGT CAACAGAGCA AGCCCGCTCT 
GGAGAGGGGC CCATGAGTAA ATTTGCCCGT GGATCAAGGT TTTCTGTGGC TGAGCATCAA 
GAGCGTTACA AAGAGGAATG TCAGCGCATC TTTGATCTAC AGAACAAGGT TTTGTCATCA 
ACTGAAATAT TGTCAACTGA CACAGACAGC AGCTCAGCCG AAGACAGTGA CTTTGAAGAA 
ATGGGAAAGA ACATTGAGAA CATGTTGCAG AATAAGAAAA CCAGCTCTCA GCTGTCACGT 
GAACGAGAGG AGCAGGAGCG GAAGGAACTG CAGCGGATGC TACTGGCAGC AGGTTCTGCA 
GCATCAGGAA ACAATCACAG AGATGATGAT ACAGCTTCTG TGACCAGCCT TAACTCTTCT 
GCCACTGGCC GTTGTCTCAA AATTTATCGC ACATTTCGAG ATGAAGAAGG GAAAGAGTAT 
GTTCGCTGTG AGACAGTCCG AAAGCCTGCT GTCATTGATG CCTATGTGCG CATACGGACC 
ACAAAAGATG AGGAATTCAT TCGAAAGTTT GCCCTTTTTG ATGAACAGCA TCGAGAAGAG 
ATGCGAAAGG AACGGCGGAG GATTCAAGAG CAACTTAGGC GGCTCAAGCG GAATCAGGAA 
AAGGAGAAGC TTAAGGGTCC CCCTGAGAAG AAGCCTAAAA AAATGAAGGA GCGTCCTGAT 
CTAAAACTGA AATGTGGAGC TTGTGGTGCC ATTGGGCACA TGAGGACAAA CAAGTTCTGC 
CCCCTCTACT ATCAAACAAA TGCTCCACCT TCCAACCCTG TTGCCATGAC AGAGGAGCAG 
GAGGAGGAGT TGGAAAAGAC AGTCATTCAT AATGATAATG AAGAACTTAT CAAGGTTGAA 
GGGACCAAGA TCGTCCTGGG AAAACAGCTA ATTGAGAGTG CAGATGAGGT TCGAAGAAAA 
TCTCTGGTTC TCAAGTTCCC TAAACAGCAA CTTCCTCCCA AGAAGAAACG GCGAGTTGGA 
ACCACTGTTC ACTGTGACTA CTTAAATAGA CCTCATAAAT CCATCCACCG GCGCCGGACA 
GACCCAATGG TGACATTGTC CTCCATCTTG GAGTCTATCA TCAATGACAT GAGAGATCTT 
CCAAATACCT ACCCTTTCCA CACTCCAGTC AATGCAAAGG TTGTAAAGGA CTACTACAAA 
ATCATCACTC GACCAATGGA CTTACAAACA CTCCGTGAAA ATGTGCGCAA ACGCCTTTAC 
CCATCTCGGG AAGAGTTCAG AGAACATTTG GAGCTCATTG TGAAAAATAG TGCAACCTAT 
AATGGTCCGA AGCACTCATT GACCCAGATC TCTCAATCTA TGCTGGATCT CTGTGATGAA 
AAACTCAAAG AGAAAGAAGA TAAATTGGCT CGTTTAGAGA AAGCTATCAA CCCCTTGCTG 
GATGATGATG ACCAAGTAGC ATTTTCTTTC ATTCTGGACA ACATTGTCAC CCAGAAAATG 
ATGGCAGTTC CAGATTCTTG GCCATTTCAT CACCCAGTTA ATAAGAAGTT TGTTCCAGAT 
TATTACAAAG TGATTATCAG TCCAATGGAT TTAGAGACTA TACGTAAGAA TATCTCCAAG 
CACAAGTACC AGAGTCGGGA GAGCTTTCTG GATGATGTCA ACCTTATTCT GGCCAACAGT 
GTTAAGTATA ATGGGCCTGA AAGTCAGTAT ACTAAGACTG CTCAGGAGAT TGTAAATGTC 
TGTTACCAGA CATTGACTGA GTATGATGAA CATTTGACTC AACTTGAGAA GGATATTTGT 
ACAGCTAAGG AAGCGGCTTT GGAGGAAGCA GAATTAGAAA GCTTGGACCC AATGACCCCA 
GGGCCTTATA CACCTCAGGC TAAGCCTCCT GATTTGTATG ACACCAACAC ATCCCTCAGT 
ATGTCCCGAG ATGCCTCTGT ATTTCAAGAT GAGAGCAATA TGTCTGTCCT GGATATTCCC 
ACTGCCACTC CAGAAAAGCA GGTGACACAG GAAGGTGAAG ATGCAGATGG TGATCTTGCA 
GATGAAGAGG AAGGAAGTAT GCAACAGCCT CAAGCCAGTG TCCTGTATGA GGATTTGCTT 
ATGTCTGAAG GAGAAGATGA TGAAGAAGAT GCTGGGAGTG ATGAAGAAGG AGACAATCCT 
TTCTCTGCTA TCCAACTGAG TGAAAGTGGA AGTGACTCTG ATGTTGGATC TAGTGGGATA 
AGACCCAAAC AGCCCCGCAT GCTTCAGGAA AACACAAGGA TGGGCATGGA AAATGAAGAA 
AGCATGATGT CCTATGAGGG AGATGGTGGG GAGGCTTCTC ATGGTTTGGA GGATAGCAAT 
ATGAGTTATG GGAGCTATGA GGAGCCTGAT CCCAAGTCGA ACACCCAAGA TACAAGCTTC 
AGCAGCATCG GTGGGTATGA GGTATCAGAG GAAGAAGAAG ATGAGGAGGA AGAGCAGCGC 
TCTGGGCCAA GTGTACTAAG CCAGGTCCAC CTGTCAGAGG ATGAGGAGGA CAGTGAGGAT 
TTCCACTCCA TTGCTGGGGA CAGTGATTTG GACTCTGATG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005641534.1
CDS	1..5685
Translation	MGPGWDLLLPVAAGDTTSAIMSDTDSDEDSAGGGPFSLTGFLFGNINGAGQLEGESVLDDECKKHLAGLGALGLGSLITELTANEELAGTDGALVNDEGWIRSTEDAVDYSDITEVAEDESRRYQQTMGSLQPLCHSDYDEDDYDADCEDIDCKLMPPPPPPPGPMKKDKDQDGLTGVSEDGEGIILPSIIAPSSLASEKVDFSSSSDSESEMGPQEASQAESEDGKLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSARRKRKKKHRELIQEEQIQEVECSVESEVNKKSLWNYDYAPPPPPEQCLSDDEITMMAPVESKFSQSTGDIDKVTDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKLRKMDHEPVIKCRMMEDLRKFEENNGSDLLADENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDKPWYSIFPIDNEELVYGRWEDNIIWDAQAMPRLLEPPVLTLDPNDENLILEIPDEKEEATSNSPSKENKKESSLKKSRILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYYIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYYSMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEILSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMLLAAGSAASGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKPAVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIISPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNGPESQYTKTAQEIVNVCYQTLTEYDEHLTQLEKDICTAKEAALEEAELESLDPMTPGPYTPQAKPPDLYDTNTSLSMSRDASVFQDESNMSVLDIPTATPEKQVTQEGEDADGDLADEEEGSMQQPQASVLYEDLLMSEGEDDEEDAGSDEEGDNPFSAIQLSESGSDSDVGSSGIRPKQPRMLQENTRMGMENEESMMSYEGDGGEASHGLEDSNMSYGSYEEPDPKSNTQDTSFSSIGGYEVSEEEEDEEEEQRSGPSVLSQVHLSEDEEDSEDFHSIAGDSDLDSDE

Target ORF information:

RefSeq Version	XM_005641477.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris TATA-box binding protein associated factor 1 (TAF1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005641477.3

ORF Insert Sequence:

ATGGGACCTG GCTGGGATTT GCTGCTGCCT GTAGCCGCCG GAGACACTAC CAGCGCCATC 
ATGTCAGACA CGGACAGCGA TGAAGATTCT GCTGGAGGCG GCCCATTTTC TCTAACCGGT 
TTCCTTTTTG GCAACATCAA TGGAGCCGGG CAGCTGGAGG GGGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGTC TGGGCAGCCT GATCACTGAA 
CTCACGGCAA ACGAAGAATT GGCTGGGACC GATGGTGCCC TGGTAAATGA TGAAGGATGG 
ATCAGGAGTA CAGAAGATGC TGTGGACTAT TCAGACATCA CTGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ATCAGCAGAC AATGGGGAGC TTGCAGCCCC TTTGCCACTC AGATTATGAT 
GAAGATGACT ATGATGCCGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAATGAA GAAAGATAAG GACCAGGATG GTCTTACCGG TGTGTCTGAG 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCATCACAG 
GCAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA ACACGATGCC 
ACCAAGCTGT TGCCAAGTGT CACAGAACTT TTCCCGGAAT TTCGGCCTGG GAAGGTATTA 
CGCTTCCTTC GTCTTTTCGG ACCAGGGAAG AATGTCCCAT CTGTTTGGCG GAGTGCTCGG 
AGAAAGAGGA AGAAGAAGCA CCGTGAGCTG ATACAGGAAG AACAGATCCA GGAGGTGGAG 
TGCTCAGTAG AGTCCGAAGT CAACAAGAAG TCTTTATGGA ATTATGACTA TGCTCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATTACAA TGATGGCTCC TGTTGAATCA 
AAGTTTTCCC AGTCAACTGG AGATATAGAT AAAGTGACAG ATACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ATTATGGCTT CAAACTGAGA AAAATGGACC ATGAGCCTGT GATAAAATGT 
AGAATGATGG AGGACTTGAG GAAATTTGAG GAAAACAATG GCTCTGACCT ACTGGCTGAT 
GAAAACTTCC TGATGGTGAC ACAGCTGCAC TGGGAGGATG ATATCATCTG GGATGGGGAG 
GATGTCAAGC ACAAAGGAAC AAAGCCTCAG CGTGCAAGCC TGGCAGGCTG GCTTCCTTCC 
AGCATGACTA GGAATGCCAT GGCCTACAAT GTTCAGCAAG GTTTTGCAGC CACCCTGGAT 
GATGACAAAC CTTGGTACTC CATCTTTCCC ATTGACAACG AGGAGCTGGT ATATGGACGC 
TGGGAGGACA ATATCATTTG GGATGCTCAG GCCATGCCCA GGCTGTTGGA GCCTCCTGTT 
TTGACACTTG ATCCCAATGA TGAGAACCTT ATTTTGGAAA TTCCTGATGA AAAGGAAGAG 
GCCACTTCTA ACTCCCCCTC CAAGGAAAAT AAGAAAGAAT CATCTCTGAA GAAGAGCCGA 
ATTCTCTTAG GGAAAACAGG GGTCATCAAG GAGGAACCAC AGCAGAACAT GTCTCAGCCA 
GAAGTGAAAG ATCCATGGAA TCTCTCCAAT GATGAATATT ACTACCCCAA GCAACAGGGT 
CTTCGAGGCA CCTTTGGAGG AAATATTATC CAGCACTCAA TTCCTGCAGT GGAATTACGA 
CAGCCCTTCT TTCCTACCCA TATGGGGCCC ATCAAACTTC GGCAGTTCCA TCGTCCACCC 
CTGAAGAAAT ATTCCTTCGG GGCACTCTCT CAGCCAGGTC CCCACTCAGT CCAGCCCTTG 
CTGAAGCACA TCAAAAAGAA GGCTAAGATG AGAGAACAAG AGAGACAAGC TTCTGGTGGT 
GGAGAGATGT TTTTTATGCG CACACCTCAG GACCTCACAG GCAAAGATGG AGATCTTATT 
CTTGCAGAAT ACAGTGAGGA AAATGGACCA TTAATGATGC AGGTTGGCAT GGCAACCAAG 
ATAAAAAACT ATTATAAGCG GAAACCTGGA AAAGATCCTG GGGCCCCAGA TTGTAAATAT 
GGAGAAACTG TTTACTGTCA TACATCTCCT TTCCTAGGCT CTCTTCATCC TGGTCAATTA 
CTGCAGGCAT TTGAGAACAA TCTCTTTCGT GCCCCAATTT ATCTTCATAA GATGCCAGAA 
ACTGACTTCC TTATTATTCG GACAAGACAA GGTTACTACA TTCGGGAGTT AGTGGATATT 
TTTGTGGTCG GCCAGCAATG CCCCTTGTTT GAAGTTCCTG GGCCCAACTC CAAAAGAGCT 
AATACACATA TTCGAGACTT TCTCCAGGTT TTTATTTACC GCCTCTTCTG GAAGAGTAAA 
GATCGGCCAA GGCGGATCCG AATGGAAGAT ATAAAAAAAG CCTTTCCTTC TCACTCAGAA 
AGTAGCATCC GCAAGAGGCT AAAGCTCTGC GCTGACTTCA AACGCACAGG GATGGACTCA 
AACTGGTGGG TGCTAAAGTC AGATTTTCGT TTACCAACTG AAGAGGAGAT CAGAGCTATG 
GTGTCTCCAG AGCAGTGCTG TGCTTATTAT AGCATGATAG CTGCAGAGCA GCGACTAAAG 
GATGCTGGCT ATGGTGAGAA GTCCTTTTTT GCTCCAGAAG AAGAAAATGA GGAAGATTTC 
CAGATGAAGA TTGATGATGA AGTTCGCACT GCTCCTTGGA ATACCACAAG GGCTTTCATT 
GCTGCCATGA AGGGCAAGTG TCTCTTGGAG GTGACTGGGG TGGCAGATCC CACAGGGTGT 
GGTGAAGGAT TCTCCTATGT CAAGATTCCA AACAAACCAA CACAGCAGAA GGATGATAAG 
GAGCCTCAGC CAGTGAAGAA GACAGTGACA GGAACAGATG CAGATCTCCG TCGCCTCTCA 
CTGAAAAATG CCAAGCAGCT CCTACGTAAA TTTGGTGTGC CTGAGGAAGA GATTAAAAAG 
CTGTCCCGCT GGGAAGTGAT TGATGTGGTA CGCACAATGT CAACAGAGCA AGCCCGCTCT 
GGAGAGGGGC CCATGAGTAA ATTTGCCCGT GGATCAAGGT TTTCTGTGGC TGAGCATCAA 
GAGCGTTACA AAGAGGAATG TCAGCGCATC TTTGATCTAC AGAACAAGGT TTTGTCATCA 
ACTGAAATAT TGTCAACTGA CACAGACAGC AGCTCAGCCG AAGACAGTGA CTTTGAAGAA 
ATGGGAAAGA ACATTGAGAA CATGTTGCAG AATAAGAAAA CCAGCTCTCA GCTGTCACGT 
GAACGAGAGG AGCAGGAGCG GAAGGAACTG CAGCGGATGC TACTGGCAGC AGGTTCTGCA 
GCATCAGGAA ACAATCACAG AGATGATGAT ACAGCTTCTG TGACCAGCCT TAACTCTTCT 
GCCACTGGCC GTTGTCTCAA AATTTATCGC ACATTTCGAG ATGAAGAAGG GAAAGAGTAT 
GTTCGCTGTG AGACAGTCCG AAAGCCTGCT GTCATTGATG CCTATGTGCG CATACGGACC 
ACAAAAGATG AGGAATTCAT TCGAAAGTTT GCCCTTTTTG ATGAACAGCA TCGAGAAGAG 
ATGCGAAAGG AACGGCGGAG GATTCAAGAG CAACTTAGGC GGCTCAAGCG GAATCAGGAA 
AAGGAGAAGC TTAAGGGTCC CCCTGAGAAG AAGCCTAAAA AAATGAAGGA GCGTCCTGAT 
CTAAAACTGA AATGTGGAGC TTGTGGTGCC ATTGGGCACA TGAGGACAAA CAAGTTCTGC 
CCCCTCTACT ATCAAACAAA TGCTCCACCT TCCAACCCTG TTGCCATGAC AGAGGAGCAG 
GAGGAGGAGT TGGAAAAGAC AGTCATTCAT AATGATAATG AAGAACTTAT CAAGGTTGAA 
GGGACCAAGA TCGTCCTGGG AAAACAGCTA ATTGAGAGTG CAGATGAGGT TCGAAGAAAA 
TCTCTGGTTC TCAAGTTCCC TAAACAGCAA CTTCCTCCCA AGAAGAAACG GCGAGTTGGA 
ACCACTGTTC ACTGTGACTA CTTAAATAGA CCTCATAAAT CCATCCACCG GCGCCGGACA 
GACCCAATGG TGACATTGTC CTCCATCTTG GAGTCTATCA TCAATGACAT GAGAGATCTT 
CCAAATACCT ACCCTTTCCA CACTCCAGTC AATGCAAAGG TTGTAAAGGA CTACTACAAA 
ATCATCACTC GACCAATGGA CTTACAAACA CTCCGTGAAA ATGTGCGCAA ACGCCTTTAC 
CCATCTCGGG AAGAGTTCAG AGAACATTTG GAGCTCATTG TGAAAAATAG TGCAACCTAT 
AATGGTCCGA AGCACTCATT GACCCAGATC TCTCAATCTA TGCTGGATCT CTGTGATGAA 
AAACTCAAAG AGAAAGAAGA TAAATTGGCT CGTTTAGAGA AAGCTATCAA CCCCTTGCTG 
GATGATGATG ACCAAGTAGC ATTTTCTTTC ATTCTGGACA ACATTGTCAC CCAGAAAATG 
ATGGCAGTTC CAGATTCTTG GCCATTTCAT CACCCAGTTA ATAAGAAGTT TGTTCCAGAT 
TATTACAAAG TGATTATCAG TCCAATGGAT TTAGAGACTA TACGTAAGAA TATCTCCAAG 
CACAAGTACC AGAGTCGGGA GAGCTTTCTG GATGATGTCA ACCTTATTCT GGCCAACAGT 
GTTAAGTATA ATGGGCCTGA AAGTCAGTAT ACTAAGACTG CTCAGGAGAT TGTAAATGTC 
TGTTACCAGA CATTGACTGA GTATGATGAA CATTTGACTC AACTTGAGAA GGATATTTGT 
ACAGCTAAGG AAGCGGCTTT GGAGGAAGCA GAATTAGAAA GCTTGGACCC AATGACCCCA 
GGGCCTTATA CACCTCAGGC TAAGCCTCCT GATTTGTATG ACACCAACAC ATCCCTCAGT 
ATGTCCCGAG ATGCCTCTGT ATTTCAAGAT GAGAGCAATA TGTCTGTCCT GGATATTCCC 
ACTGCCACTC CAGAAAAGCA GGTGACACAG GAAGGTGAAG ATGCAGATGG TGATCTTGCA 
GATGAAGAGG AAGGAAGTAT GCAACAGCCT CAAGCCAGTG TCCTGTATGA GGATTTGCTT 
ATGTCTGAAG GAGAAGATGA TGAAGAAGAT GCTGGGAGTG ATGAAGAAGG AGACAATCCT 
TTCTCTGCTA TCCAACTGAG TGAAAGTGGA AGTGACTCTG ATGTTGGATC TAGTGGGATA 
AGACCCAAAC AGCCCCGCAT GCTTCAGGAA AACACAAGGA TGGGCATGGA AAATGAAGAA 
AGCATGATGT CCTATGAGGG AGATGGTGGG GAGGCTTCTC ATGGTTTGGA GGATAGCAAT 
ATGAGTTATG GGAGCTATGA GGAGCCTGAT CCCAAGTCGA ACACCCAAGA TACAAGCTTC 
AGCAGCATCG GTGGGTATGA GGTATCAGAG GAAGAAGAAG ATGAGGAGGA AGAGCAGCGC 
TCTGGGCCAA GTGTACTAAG CCAGGTCCAC CTGTCAGAGG ATGAGGAGGA CAGTGAGGAT 
TTCCACTCCA TTGCTGGGGA CAGTGATTTG GACTCTGATG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAF1 ( XM_005641477.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005641534.1 Canis lupus familiaris TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa (TAF1), transcript variant X5, mRNA.

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CloneID	OCe32531
Clone ID Related Accession (Same CDS sequence)	XM_005641477.3 , XM_005641477.2
Accession Version	XM_005641477.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	transcription initiation factor TFIID subunit 1 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005641477.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACCTG GCTGGGATTT GCTGCTGCCT GTAGCCGCCG GAGACACTAC CAGCGCCATC 
ATGTCAGACA CGGACAGCGA TGAAGATTCT GCTGGAGGCG GCCCATTTTC TCTAACCGGT 
TTCCTTTTTG GCAACATCAA TGGAGCCGGG CAGCTGGAGG GGGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGTC TGGGCAGCCT GATCACTGAA 
CTCACGGCAA ACGAAGAATT GGCTGGGACC GATGGTGCCC TGGTAAATGA TGAAGGATGG 
ATCAGGAGTA CAGAAGATGC TGTGGACTAT TCAGACATCA CTGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ATCAGCAGAC AATGGGGAGC TTGCAGCCCC TTTGCCACTC AGATTATGAT 
GAAGATGACT ATGATGCCGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAATGAA GAAAGATAAG GACCAGGATG GTCTTACCGG TGTGTCTGAG 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCATCACAG 
GCAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA ACACGATGCC 
ACCAAGCTGT TGCCAAGTGT CACAGAACTT TTCCCGGAAT TTCGGCCTGG GAAGGTATTA 
CGCTTCCTTC GTCTTTTCGG ACCAGGGAAG AATGTCCCAT CTGTTTGGCG GAGTGCTCGG 
AGAAAGAGGA AGAAGAAGCA CCGTGAGCTG ATACAGGAAG AACAGATCCA GGAGGTGGAG 
TGCTCAGTAG AGTCCGAAGT CAACAAGAAG TCTTTATGGA ATTATGACTA TGCTCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATTACAA TGATGGCTCC TGTTGAATCA 
AAGTTTTCCC AGTCAACTGG AGATATAGAT AAAGTGACAG ATACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ATTATGGCTT CAAACTGAGA AAAATGGACC ATGAGCCTGT GATAAAATGT 
AGAATGATGG AGGACTTGAG GAAATTTGAG GAAAACAATG GCTCTGACCT ACTGGCTGAT 
GAAAACTTCC TGATGGTGAC ACAGCTGCAC TGGGAGGATG ATATCATCTG GGATGGGGAG 
GATGTCAAGC ACAAAGGAAC AAAGCCTCAG CGTGCAAGCC TGGCAGGCTG GCTTCCTTCC 
AGCATGACTA GGAATGCCAT GGCCTACAAT GTTCAGCAAG GTTTTGCAGC CACCCTGGAT 
GATGACAAAC CTTGGTACTC CATCTTTCCC ATTGACAACG AGGAGCTGGT ATATGGACGC 
TGGGAGGACA ATATCATTTG GGATGCTCAG GCCATGCCCA GGCTGTTGGA GCCTCCTGTT 
TTGACACTTG ATCCCAATGA TGAGAACCTT ATTTTGGAAA TTCCTGATGA AAAGGAAGAG 
GCCACTTCTA ACTCCCCCTC CAAGGAAAAT AAGAAAGAAT CATCTCTGAA GAAGAGCCGA 
ATTCTCTTAG GGAAAACAGG GGTCATCAAG GAGGAACCAC AGCAGAACAT GTCTCAGCCA 
GAAGTGAAAG ATCCATGGAA TCTCTCCAAT GATGAATATT ACTACCCCAA GCAACAGGGT 
CTTCGAGGCA CCTTTGGAGG AAATATTATC CAGCACTCAA TTCCTGCAGT GGAATTACGA 
CAGCCCTTCT TTCCTACCCA TATGGGGCCC ATCAAACTTC GGCAGTTCCA TCGTCCACCC 
CTGAAGAAAT ATTCCTTCGG GGCACTCTCT CAGCCAGGTC CCCACTCAGT CCAGCCCTTG 
CTGAAGCACA TCAAAAAGAA GGCTAAGATG AGAGAACAAG AGAGACAAGC TTCTGGTGGT 
GGAGAGATGT TTTTTATGCG CACACCTCAG GACCTCACAG GCAAAGATGG AGATCTTATT 
CTTGCAGAAT ACAGTGAGGA AAATGGACCA TTAATGATGC AGGTTGGCAT GGCAACCAAG 
ATAAAAAACT ATTATAAGCG GAAACCTGGA AAAGATCCTG GGGCCCCAGA TTGTAAATAT 
GGAGAAACTG TTTACTGTCA TACATCTCCT TTCCTAGGCT CTCTTCATCC TGGTCAATTA 
CTGCAGGCAT TTGAGAACAA TCTCTTTCGT GCCCCAATTT ATCTTCATAA GATGCCAGAA 
ACTGACTTCC TTATTATTCG GACAAGACAA GGTTACTACA TTCGGGAGTT AGTGGATATT 
TTTGTGGTCG GCCAGCAATG CCCCTTGTTT GAAGTTCCTG GGCCCAACTC CAAAAGAGCT 
AATACACATA TTCGAGACTT TCTCCAGGTT TTTATTTACC GCCTCTTCTG GAAGAGTAAA 
GATCGGCCAA GGCGGATCCG AATGGAAGAT ATAAAAAAAG CCTTTCCTTC TCACTCAGAA 
AGTAGCATCC GCAAGAGGCT AAAGCTCTGC GCTGACTTCA AACGCACAGG GATGGACTCA 
AACTGGTGGG TGCTAAAGTC AGATTTTCGT TTACCAACTG AAGAGGAGAT CAGAGCTATG 
GTGTCTCCAG AGCAGTGCTG TGCTTATTAT AGCATGATAG CTGCAGAGCA GCGACTAAAG 
GATGCTGGCT ATGGTGAGAA GTCCTTTTTT GCTCCAGAAG AAGAAAATGA GGAAGATTTC 
CAGATGAAGA TTGATGATGA AGTTCGCACT GCTCCTTGGA ATACCACAAG GGCTTTCATT 
GCTGCCATGA AGGGCAAGTG TCTCTTGGAG GTGACTGGGG TGGCAGATCC CACAGGGTGT 
GGTGAAGGAT TCTCCTATGT CAAGATTCCA AACAAACCAA CACAGCAGAA GGATGATAAG 
GAGCCTCAGC CAGTGAAGAA GACAGTGACA GGAACAGATG CAGATCTCCG TCGCCTCTCA 
CTGAAAAATG CCAAGCAGCT CCTACGTAAA TTTGGTGTGC CTGAGGAAGA GATTAAAAAG 
CTGTCCCGCT GGGAAGTGAT TGATGTGGTA CGCACAATGT CAACAGAGCA AGCCCGCTCT 
GGAGAGGGGC CCATGAGTAA ATTTGCCCGT GGATCAAGGT TTTCTGTGGC TGAGCATCAA 
GAGCGTTACA AAGAGGAATG TCAGCGCATC TTTGATCTAC AGAACAAGGT TTTGTCATCA 
ACTGAAATAT TGTCAACTGA CACAGACAGC AGCTCAGCCG AAGACAGTGA CTTTGAAGAA 
ATGGGAAAGA ACATTGAGAA CATGTTGCAG AATAAGAAAA CCAGCTCTCA GCTGTCACGT 
GAACGAGAGG AGCAGGAGCG GAAGGAACTG CAGCGGATGC TACTGGCAGC AGGTTCTGCA 
GCATCAGGAA ACAATCACAG AGATGATGAT ACAGCTTCTG TGACCAGCCT TAACTCTTCT 
GCCACTGGCC GTTGTCTCAA AATTTATCGC ACATTTCGAG ATGAAGAAGG GAAAGAGTAT 
GTTCGCTGTG AGACAGTCCG AAAGCCTGCT GTCATTGATG CCTATGTGCG CATACGGACC 
ACAAAAGATG AGGAATTCAT TCGAAAGTTT GCCCTTTTTG ATGAACAGCA TCGAGAAGAG 
ATGCGAAAGG AACGGCGGAG GATTCAAGAG CAACTTAGGC GGCTCAAGCG GAATCAGGAA 
AAGGAGAAGC TTAAGGGTCC CCCTGAGAAG AAGCCTAAAA AAATGAAGGA GCGTCCTGAT 
CTAAAACTGA AATGTGGAGC TTGTGGTGCC ATTGGGCACA TGAGGACAAA CAAGTTCTGC 
CCCCTCTACT ATCAAACAAA TGCTCCACCT TCCAACCCTG TTGCCATGAC AGAGGAGCAG 
GAGGAGGAGT TGGAAAAGAC AGTCATTCAT AATGATAATG AAGAACTTAT CAAGGTTGAA 
GGGACCAAGA TCGTCCTGGG AAAACAGCTA ATTGAGAGTG CAGATGAGGT TCGAAGAAAA 
TCTCTGGTTC TCAAGTTCCC TAAACAGCAA CTTCCTCCCA AGAAGAAACG GCGAGTTGGA 
ACCACTGTTC ACTGTGACTA CTTAAATAGA CCTCATAAAT CCATCCACCG GCGCCGGACA 
GACCCAATGG TGACATTGTC CTCCATCTTG GAGTCTATCA TCAATGACAT GAGAGATCTT 
CCAAATACCT ACCCTTTCCA CACTCCAGTC AATGCAAAGG TTGTAAAGGA CTACTACAAA 
ATCATCACTC GACCAATGGA CTTACAAACA CTCCGTGAAA ATGTGCGCAA ACGCCTTTAC 
CCATCTCGGG AAGAGTTCAG AGAACATTTG GAGCTCATTG TGAAAAATAG TGCAACCTAT 
AATGGTCCGA AGCACTCATT GACCCAGATC TCTCAATCTA TGCTGGATCT CTGTGATGAA 
AAACTCAAAG AGAAAGAAGA TAAATTGGCT CGTTTAGAGA AAGCTATCAA CCCCTTGCTG 
GATGATGATG ACCAAGTAGC ATTTTCTTTC ATTCTGGACA ACATTGTCAC CCAGAAAATG 
ATGGCAGTTC CAGATTCTTG GCCATTTCAT CACCCAGTTA ATAAGAAGTT TGTTCCAGAT 
TATTACAAAG TGATTATCAG TCCAATGGAT TTAGAGACTA TACGTAAGAA TATCTCCAAG 
CACAAGTACC AGAGTCGGGA GAGCTTTCTG GATGATGTCA ACCTTATTCT GGCCAACAGT 
GTTAAGTATA ATGGGCCTGA AAGTCAGTAT ACTAAGACTG CTCAGGAGAT TGTAAATGTC 
TGTTACCAGA CATTGACTGA GTATGATGAA CATTTGACTC AACTTGAGAA GGATATTTGT 
ACAGCTAAGG AAGCGGCTTT GGAGGAAGCA GAATTAGAAA GCTTGGACCC AATGACCCCA 
GGGCCTTATA CACCTCAGGC TAAGCCTCCT GATTTGTATG ACACCAACAC ATCCCTCAGT 
ATGTCCCGAG ATGCCTCTGT ATTTCAAGAT GAGAGCAATA TGTCTGTCCT GGATATTCCC 
ACTGCCACTC CAGAAAAGCA GGTGACACAG GAAGGTGAAG ATGCAGATGG TGATCTTGCA 
GATGAAGAGG AAGGAAGTAT GCAACAGCCT CAAGCCAGTG TCCTGTATGA GGATTTGCTT 
ATGTCTGAAG GAGAAGATGA TGAAGAAGAT GCTGGGAGTG ATGAAGAAGG AGACAATCCT 
TTCTCTGCTA TCCAACTGAG TGAAAGTGGA AGTGACTCTG ATGTTGGATC TAGTGGGATA 
AGACCCAAAC AGCCCCGCAT GCTTCAGGAA AACACAAGGA TGGGCATGGA AAATGAAGAA 
AGCATGATGT CCTATGAGGG AGATGGTGGG GAGGCTTCTC ATGGTTTGGA GGATAGCAAT 
ATGAGTTATG GGAGCTATGA GGAGCCTGAT CCCAAGTCGA ACACCCAAGA TACAAGCTTC 
AGCAGCATCG GTGGGTATGA GGTATCAGAG GAAGAAGAAG ATGAGGAGGA AGAGCAGCGC 
TCTGGGCCAA GTGTACTAAG CCAGGTCCAC CTGTCAGAGG ATGAGGAGGA CAGTGAGGAT 
TTCCACTCCA TTGCTGGGGA CAGTGATTTG GACTCTGATG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005641534.1
CDS	93..5777
Translation	MGPGWDLLLPVAAGDTTSAIMSDTDSDEDSAGGGPFSLTGFLFGNINGAGQLEGESVLDDECKKHLAGLGALGLGSLITELTANEELAGTDGALVNDEGWIRSTEDAVDYSDITEVAEDESRRYQQTMGSLQPLCHSDYDEDDYDADCEDIDCKLMPPPPPPPGPMKKDKDQDGLTGVSEDGEGIILPSIIAPSSLASEKVDFSSSSDSESEMGPQEASQAESEDGKLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSARRKRKKKHRELIQEEQIQEVECSVESEVNKKSLWNYDYAPPPPPEQCLSDDEITMMAPVESKFSQSTGDIDKVTDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKLRKMDHEPVIKCRMMEDLRKFEENNGSDLLADENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDKPWYSIFPIDNEELVYGRWEDNIIWDAQAMPRLLEPPVLTLDPNDENLILEIPDEKEEATSNSPSKENKKESSLKKSRILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYYIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYYSMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEILSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMLLAAGSAASGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKPAVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIISPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNGPESQYTKTAQEIVNVCYQTLTEYDEHLTQLEKDICTAKEAALEEAELESLDPMTPGPYTPQAKPPDLYDTNTSLSMSRDASVFQDESNMSVLDIPTATPEKQVTQEGEDADGDLADEEEGSMQQPQASVLYEDLLMSEGEDDEEDAGSDEEGDNPFSAIQLSESGSDSDVGSSGIRPKQPRMLQENTRMGMENEESMMSYEGDGGEASHGLEDSNMSYGSYEEPDPKSNTQDTSFSSIGGYEVSEEEEDEEEEQRSGPSVLSQVHLSEDEEDSEDFHSIAGDSDLDSDE

Target ORF information:

RefSeq Version	XM_005641477.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa (TAF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005641477.2

ORF Insert Sequence:

ATGGGACCTG GCTGGGATTT GCTGCTGCCT GTAGCCGCCG GAGACACTAC CAGCGCCATC 
ATGTCAGACA CGGACAGCGA TGAAGATTCT GCTGGAGGCG GCCCATTTTC TCTAACCGGT 
TTCCTTTTTG GCAACATCAA TGGAGCCGGG CAGCTGGAGG GGGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGTC TGGGCAGCCT GATCACTGAA 
CTCACGGCAA ACGAAGAATT GGCTGGGACC GATGGTGCCC TGGTAAATGA TGAAGGATGG 
ATCAGGAGTA CAGAAGATGC TGTGGACTAT TCAGACATCA CTGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ATCAGCAGAC AATGGGGAGC TTGCAGCCCC TTTGCCACTC AGATTATGAT 
GAAGATGACT ATGATGCCGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAATGAA GAAAGATAAG GACCAGGATG GTCTTACCGG TGTGTCTGAG 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCATCACAG 
GCAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA ACACGATGCC 
ACCAAGCTGT TGCCAAGTGT CACAGAACTT TTCCCGGAAT TTCGGCCTGG GAAGGTATTA 
CGCTTCCTTC GTCTTTTCGG ACCAGGGAAG AATGTCCCAT CTGTTTGGCG GAGTGCTCGG 
AGAAAGAGGA AGAAGAAGCA CCGTGAGCTG ATACAGGAAG AACAGATCCA GGAGGTGGAG 
TGCTCAGTAG AGTCCGAAGT CAACAAGAAG TCTTTATGGA ATTATGACTA TGCTCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATTACAA TGATGGCTCC TGTTGAATCA 
AAGTTTTCCC AGTCAACTGG AGATATAGAT AAAGTGACAG ATACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ATTATGGCTT CAAACTGAGA AAAATGGACC ATGAGCCTGT GATAAAATGT 
AGAATGATGG AGGACTTGAG GAAATTTGAG GAAAACAATG GCTCTGACCT ACTGGCTGAT 
GAAAACTTCC TGATGGTGAC ACAGCTGCAC TGGGAGGATG ATATCATCTG GGATGGGGAG 
GATGTCAAGC ACAAAGGAAC AAAGCCTCAG CGTGCAAGCC TGGCAGGCTG GCTTCCTTCC 
AGCATGACTA GGAATGCCAT GGCCTACAAT GTTCAGCAAG GTTTTGCAGC CACCCTGGAT 
GATGACAAAC CTTGGTACTC CATCTTTCCC ATTGACAACG AGGAGCTGGT ATATGGACGC 
TGGGAGGACA ATATCATTTG GGATGCTCAG GCCATGCCCA GGCTGTTGGA GCCTCCTGTT 
TTGACACTTG ATCCCAATGA TGAGAACCTT ATTTTGGAAA TTCCTGATGA AAAGGAAGAG 
GCCACTTCTA ACTCCCCCTC CAAGGAAAAT AAGAAAGAAT CATCTCTGAA GAAGAGCCGA 
ATTCTCTTAG GGAAAACAGG GGTCATCAAG GAGGAACCAC AGCAGAACAT GTCTCAGCCA 
GAAGTGAAAG ATCCATGGAA TCTCTCCAAT GATGAATATT ACTACCCCAA GCAACAGGGT 
CTTCGAGGCA CCTTTGGAGG AAATATTATC CAGCACTCAA TTCCTGCAGT GGAATTACGA 
CAGCCCTTCT TTCCTACCCA TATGGGGCCC ATCAAACTTC GGCAGTTCCA TCGTCCACCC 
CTGAAGAAAT ATTCCTTCGG GGCACTCTCT CAGCCAGGTC CCCACTCAGT CCAGCCCTTG 
CTGAAGCACA TCAAAAAGAA GGCTAAGATG AGAGAACAAG AGAGACAAGC TTCTGGTGGT 
GGAGAGATGT TTTTTATGCG CACACCTCAG GACCTCACAG GCAAAGATGG AGATCTTATT 
CTTGCAGAAT ACAGTGAGGA AAATGGACCA TTAATGATGC AGGTTGGCAT GGCAACCAAG 
ATAAAAAACT ATTATAAGCG GAAACCTGGA AAAGATCCTG GGGCCCCAGA TTGTAAATAT 
GGAGAAACTG TTTACTGTCA TACATCTCCT TTCCTAGGCT CTCTTCATCC TGGTCAATTA 
CTGCAGGCAT TTGAGAACAA TCTCTTTCGT GCCCCAATTT ATCTTCATAA GATGCCAGAA 
ACTGACTTCC TTATTATTCG GACAAGACAA GGTTACTACA TTCGGGAGTT AGTGGATATT 
TTTGTGGTCG GCCAGCAATG CCCCTTGTTT GAAGTTCCTG GGCCCAACTC CAAAAGAGCT 
AATACACATA TTCGAGACTT TCTCCAGGTT TTTATTTACC GCCTCTTCTG GAAGAGTAAA 
GATCGGCCAA GGCGGATCCG AATGGAAGAT ATAAAAAAAG CCTTTCCTTC TCACTCAGAA 
AGTAGCATCC GCAAGAGGCT AAAGCTCTGC GCTGACTTCA AACGCACAGG GATGGACTCA 
AACTGGTGGG TGCTAAAGTC AGATTTTCGT TTACCAACTG AAGAGGAGAT CAGAGCTATG 
GTGTCTCCAG AGCAGTGCTG TGCTTATTAT AGCATGATAG CTGCAGAGCA GCGACTAAAG 
GATGCTGGCT ATGGTGAGAA GTCCTTTTTT GCTCCAGAAG AAGAAAATGA GGAAGATTTC 
CAGATGAAGA TTGATGATGA AGTTCGCACT GCTCCTTGGA ATACCACAAG GGCTTTCATT 
GCTGCCATGA AGGGCAAGTG TCTCTTGGAG GTGACTGGGG TGGCAGATCC CACAGGGTGT 
GGTGAAGGAT TCTCCTATGT CAAGATTCCA AACAAACCAA CACAGCAGAA GGATGATAAG 
GAGCCTCAGC CAGTGAAGAA GACAGTGACA GGAACAGATG CAGATCTCCG TCGCCTCTCA 
CTGAAAAATG CCAAGCAGCT CCTACGTAAA TTTGGTGTGC CTGAGGAAGA GATTAAAAAG 
CTGTCCCGCT GGGAAGTGAT TGATGTGGTA CGCACAATGT CAACAGAGCA AGCCCGCTCT 
GGAGAGGGGC CCATGAGTAA ATTTGCCCGT GGATCAAGGT TTTCTGTGGC TGAGCATCAA 
GAGCGTTACA AAGAGGAATG TCAGCGCATC TTTGATCTAC AGAACAAGGT TTTGTCATCA 
ACTGAAATAT TGTCAACTGA CACAGACAGC AGCTCAGCCG AAGACAGTGA CTTTGAAGAA 
ATGGGAAAGA ACATTGAGAA CATGTTGCAG AATAAGAAAA CCAGCTCTCA GCTGTCACGT 
GAACGAGAGG AGCAGGAGCG GAAGGAACTG CAGCGGATGC TACTGGCAGC AGGTTCTGCA 
GCATCAGGAA ACAATCACAG AGATGATGAT ACAGCTTCTG TGACCAGCCT TAACTCTTCT 
GCCACTGGCC GTTGTCTCAA AATTTATCGC ACATTTCGAG ATGAAGAAGG GAAAGAGTAT 
GTTCGCTGTG AGACAGTCCG AAAGCCTGCT GTCATTGATG CCTATGTGCG CATACGGACC 
ACAAAAGATG AGGAATTCAT TCGAAAGTTT GCCCTTTTTG ATGAACAGCA TCGAGAAGAG 
ATGCGAAAGG AACGGCGGAG GATTCAAGAG CAACTTAGGC GGCTCAAGCG GAATCAGGAA 
AAGGAGAAGC TTAAGGGTCC CCCTGAGAAG AAGCCTAAAA AAATGAAGGA GCGTCCTGAT 
CTAAAACTGA AATGTGGAGC TTGTGGTGCC ATTGGGCACA TGAGGACAAA CAAGTTCTGC 
CCCCTCTACT ATCAAACAAA TGCTCCACCT TCCAACCCTG TTGCCATGAC AGAGGAGCAG 
GAGGAGGAGT TGGAAAAGAC AGTCATTCAT AATGATAATG AAGAACTTAT CAAGGTTGAA 
GGGACCAAGA TCGTCCTGGG AAAACAGCTA ATTGAGAGTG CAGATGAGGT TCGAAGAAAA 
TCTCTGGTTC TCAAGTTCCC TAAACAGCAA CTTCCTCCCA AGAAGAAACG GCGAGTTGGA 
ACCACTGTTC ACTGTGACTA CTTAAATAGA CCTCATAAAT CCATCCACCG GCGCCGGACA 
GACCCAATGG TGACATTGTC CTCCATCTTG GAGTCTATCA TCAATGACAT GAGAGATCTT 
CCAAATACCT ACCCTTTCCA CACTCCAGTC AATGCAAAGG TTGTAAAGGA CTACTACAAA 
ATCATCACTC GACCAATGGA CTTACAAACA CTCCGTGAAA ATGTGCGCAA ACGCCTTTAC 
CCATCTCGGG AAGAGTTCAG AGAACATTTG GAGCTCATTG TGAAAAATAG TGCAACCTAT 
AATGGTCCGA AGCACTCATT GACCCAGATC TCTCAATCTA TGCTGGATCT CTGTGATGAA 
AAACTCAAAG AGAAAGAAGA TAAATTGGCT CGTTTAGAGA AAGCTATCAA CCCCTTGCTG 
GATGATGATG ACCAAGTAGC ATTTTCTTTC ATTCTGGACA ACATTGTCAC CCAGAAAATG 
ATGGCAGTTC CAGATTCTTG GCCATTTCAT CACCCAGTTA ATAAGAAGTT TGTTCCAGAT 
TATTACAAAG TGATTATCAG TCCAATGGAT TTAGAGACTA TACGTAAGAA TATCTCCAAG 
CACAAGTACC AGAGTCGGGA GAGCTTTCTG GATGATGTCA ACCTTATTCT GGCCAACAGT 
GTTAAGTATA ATGGGCCTGA AAGTCAGTAT ACTAAGACTG CTCAGGAGAT TGTAAATGTC 
TGTTACCAGA CATTGACTGA GTATGATGAA CATTTGACTC AACTTGAGAA GGATATTTGT 
ACAGCTAAGG AAGCGGCTTT GGAGGAAGCA GAATTAGAAA GCTTGGACCC AATGACCCCA 
GGGCCTTATA CACCTCAGGC TAAGCCTCCT GATTTGTATG ACACCAACAC ATCCCTCAGT 
ATGTCCCGAG ATGCCTCTGT ATTTCAAGAT GAGAGCAATA TGTCTGTCCT GGATATTCCC 
ACTGCCACTC CAGAAAAGCA GGTGACACAG GAAGGTGAAG ATGCAGATGG TGATCTTGCA 
GATGAAGAGG AAGGAAGTAT GCAACAGCCT CAAGCCAGTG TCCTGTATGA GGATTTGCTT 
ATGTCTGAAG GAGAAGATGA TGAAGAAGAT GCTGGGAGTG ATGAAGAAGG AGACAATCCT 
TTCTCTGCTA TCCAACTGAG TGAAAGTGGA AGTGACTCTG ATGTTGGATC TAGTGGGATA 
AGACCCAAAC AGCCCCGCAT GCTTCAGGAA AACACAAGGA TGGGCATGGA AAATGAAGAA 
AGCATGATGT CCTATGAGGG AGATGGTGGG GAGGCTTCTC ATGGTTTGGA GGATAGCAAT 
ATGAGTTATG GGAGCTATGA GGAGCCTGAT CCCAAGTCGA ACACCCAAGA TACAAGCTTC 
AGCAGCATCG GTGGGTATGA GGTATCAGAG GAAGAAGAAG ATGAGGAGGA AGAGCAGCGC 
TCTGGGCCAA GTGTACTAAG CCAGGTCCAC CTGTCAGAGG ATGAGGAGGA CAGTGAGGAT 
TTCCACTCCA TTGCTGGGGA CAGTGATTTG GACTCTGATG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAF1 ( XM_003435561.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003435609.1 Canis lupus familiaris TATA-box binding protein associated factor 1 (TAF1), transcript variant X7, mRNA.

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CloneID	OCe32532
Clone ID Related Accession (Same CDS sequence)	XM_003435561.4 , XM_003435561.3
Accession Version	XM_003435561.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5679bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	transcription initiation factor TFIID subunit 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003435561.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACCTG GCTGGGATTT GCTGCTGCCT GTAGCCGCCG GAGACACTAC CAGCGCCATC 
ATGTCAGACA CGGACAGCGA TGAAGATTCT GCTGGAGGCG GCCCATTTTC TCTAACCGGT 
TTCCTTTTTG GCAACATCAA TGGAGCCGGG CAGCTGGAGG GGGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGTC TGGGCAGCCT GATCACTGAA 
CTCACGGCAA ACGAAGAATT GGCTGGGACC GATGGTGCCC TGGTAAATGA TGAAGGATGG 
ATCAGGAGTA CAGAAGATGC TGTGGACTAT TCAGACATCA CTGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ATCAGCAGAC AATGGGGAGC TTGCAGCCCC TTTGCCACTC AGATTATGAT 
GAAGATGACT ATGATGCCGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAATGAA GAAAGATAAG GACCAGGATG GTCTTACCGG TGTGTCTGAG 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCATCACAG 
GCAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA ACACGATGCC 
ACCAAGCTGT TGCCAAGTGT CACAGAACTT TTCCCGGAAT TTCGGCCTGG GAAGGTATTA 
CGCTTCCTTC GTCTTTTCGG ACCAGGGAAG AATGTCCCAT CTGTTTGGCG GAGTGCTCGG 
AGAAAGAGGA AGAAGAAGCA CCGTGAGCTG ATACAGGAAG AACAGATCCA GGAGGTGGAG 
TGCTCAGTAG AGTCCGAAGT CAACAAGAAG TCTTTATGGA ATTATGACTA TGCTCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATTACAA TGATGGCTCC TGTTGAATCA 
AAGTTTTCCC AGTCAACTGG AGATATAGAT AAAGTGACAG ATACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ATTATGGCTT CAAACTGAGA AAAATGGACC ATGAGCCTGT GATAAAATGT 
AGAATGATGG AGGACTTGAG GAAATTTGAG GAAAACAATG GCTCTGACCT ACTGGCTGAT 
GAAAACTTCC TGATGGTGAC ACAGCTGCAC TGGGAGGATG ATATCATCTG GGATGGGGAG 
GATGTCAAGC ACAAAGGAAC AAAGCCTCAG CGTGCAAGCC TGGCAGGCTG GCTTCCTTCC 
AGCATGACTA GGAATGCCAT GGCCTACAAT GTTCAGCAAG GTTTTGCAGC CACCCTGGAT 
GATGACAAAC CTTGGTACTC CATCTTTCCC ATTGACAACG AGGAGCTGGT ATATGGACGC 
TGGGAGGACA ATATCATTTG GGATGCTCAG GCCATGCCCA GGCTGTTGGA GCCTCCTGTT 
TTGACACTTG ATCCCAATGA TGAGAACCTT ATTTTGGAAA TTCCTGATGA AAAGGAAGAG 
GCCACTTCTA ACTCCCCCTC CAAGGAAAAT AAGAAAGAAT CATCTCTGAA GAAGAGCCGA 
ATTCTCTTAG GGAAAACAGG GGTCATCAAG GAGGAACCAC AGCAGAACAT GTCTCAGCCA 
GAAGTGAAAG ATCCATGGAA TCTCTCCAAT GATGAATATT ACTACCCCAA GCAACAGGGT 
CTTCGAGGCA CCTTTGGAGG AAATATTATC CAGCACTCAA TTCCTGCAGT GGAATTACGA 
CAGCCCTTCT TTCCTACCCA TATGGGGCCC ATCAAACTTC GGCAGTTCCA TCGTCCACCC 
CTGAAGAAAT ATTCCTTCGG GGCACTCTCT CAGCCAGGTC CCCACTCAGT CCAGCCCTTG 
CTGAAGCACA TCAAAAAGAA GGCTAAGATG AGAGAACAAG AGAGACAAGC TTCTGGTGGT 
GGAGAGATGT TTTTTATGCG CACACCTCAG GACCTCACAG GCAAAGATGG AGATCTTATT 
CTTGCAGAAT ACAGTGAGGA AAATGGACCA TTAATGATGC AGGTTGGCAT GGCAACCAAG 
ATAAAAAACT ATTATAAGCG GAAACCTGGA AAAGATCCTG GGGCCCCAGA TTGTAAATAT 
GGAGAAACTG TTTACTGTCA TACATCTCCT TTCCTAGGCT CTCTTCATCC TGGTCAATTA 
CTGCAGGCAT TTGAGAACAA TCTCTTTCGT GCCCCAATTT ATCTTCATAA GATGCCAGAA 
ACTGACTTCC TTATTATTCG GACAAGACAA GGTTACTACA TTCGGGAGTT AGTGGATATT 
TTTGTGGTCG GCCAGCAATG CCCCTTGTTT GAAGTTCCTG GGCCCAACTC CAAAAGAGCT 
AATACACATA TTCGAGACTT TCTCCAGGTT TTTATTTACC GCCTCTTCTG GAAGAGTAAA 
GATCGGCCAA GGCGGATCCG AATGGAAGAT ATAAAAAAAG CCTTTCCTTC TCACTCAGAA 
AGTAGCATCC GCAAGAGGCT AAAGCTCTGC GCTGACTTCA AACGCACAGG GATGGACTCA 
AACTGGTGGG TGCTAAAGTC AGATTTTCGT TTACCAACTG AAGAGGAGAT CAGAGCTATG 
GTGTCTCCAG AGCAGTGCTG TGCTTATTAT AGCATGATAG CTGCAGAGCA GCGACTAAAG 
GATGCTGGCT ATGGTGAGAA GTCCTTTTTT GCTCCAGAAG AAGAAAATGA GGAAGATTTC 
CAGATGAAGA TTGATGATGA AGTTCGCACT GCTCCTTGGA ATACCACAAG GGCTTTCATT 
GCTGCCATGA AGGGCAAGTG TCTCTTGGAG GTGACTGGGG TGGCAGATCC CACAGGGTGT 
GGTGAAGGAT TCTCCTATGT CAAGATTCCA AACAAACCAA CACAGCAGAA GGATGATAAG 
GAGCCTCAGC CAGTGAAGAA GACAGTGACA GGAACAGATG CAGATCTCCG TCGCCTCTCA 
CTGAAAAATG CCAAGCAGCT CCTACGTAAA TTTGGTGTGC CTGAGGAAGA GATTAAAAAG 
CTGTCCCGCT GGGAAGTGAT TGATGTGGTA CGCACAATGT CAACAGAGCA AGCCCGCTCT 
GGAGAGGGGC CCATGAGTAA ATTTGCCCGT GGATCAAGGT TTTCTGTGGC TGAGCATCAA 
GAGCGTTACA AAGAGGAATG TCAGCGCATC TTTGATCTAC AGAACAAGGT TTTGTCATCA 
ACTGAAATAT TGTCAACTGA CACAGACAGC AGCTCAGCCG AAGACAGTGA CTTTGAAGAA 
ATGGGAAAGA ACATTGAGAA CATGTTGCAG AATAAGAAAA CCAGCTCTCA GCTGTCACGT 
GAACGAGAGG AGCAGGAGCG GAAGGAACTG CAGCGGATGC TACTGGCAGC AGGTTCTGCA 
GCATCAGGAA ACAATCACAG AGATGATGAT ACAGCTTCTG TGACCAGCCT TAACTCTTCT 
GCCACTGGCC GTTGTCTCAA AATTTATCGC ACATTTCGAG ATGAAGAAGG GAAAGAGTAT 
GTTCGCTGTG AGACAGTCCG AAAGCCTGCT GTCATTGATG CCTATGTGCG CATACGGACC 
ACAAAAGATG AGGAATTCAT TCGAAAGTTT GCCCTTTTTG ATGAACAGCA TCGAGAAGAG 
ATGCGAAAGG AACGGCGGAG GATTCAAGAG CAACTTAGGC GGCTCAAGCG GAATCAGGAA 
AAGGAGAAGC TTAAGGGTCC CCCTGAGAAG AAGCCTAAAA AAATGAAGGA GCGTCCTGAT 
CTAAAACTGA AATGTGGAGC TTGTGGTGCC ATTGGGCACA TGAGGACAAA CAAGTTCTGC 
CCCCTCTACT ATCAAACAAA TGCTCCACCT TCCAACCCTG TTGCCATGAC AGAGGAGCAG 
GAGGAGGAGT TGGAAAAGAC AGTCATTCAT AATGATAATG AAGAACTTAT CAAGGTTGAA 
GGGACCAAGA TCGTCCTGGG AAAACAGCTA ATTGAGAGTG CAGATGAGGT TCGAAGAAAA 
TCTCTGGTTC TCAAGTTCCC TAAACAGCAA CTTCCTCCCA AGAAGAAACG GCGAGTTGGA 
ACCACTGTTC ACTGTGACTA CTTAAATAGA CCTCATAAAT CCATCCACCG GCGCCGGACA 
GACCCAATGG TGACATTGTC CTCCATCTTG GAGTCTATCA TCAATGACAT GAGAGATCTT 
CCAAATACCT ACCCTTTCCA CACTCCAGTC AATGCAAAGG TTGTAAAGGA CTACTACAAA 
ATCATCACTC GACCAATGGA CTTACAAACA CTCCGTGAAA ATGTGCGCAA ACGCCTTTAC 
CCATCTCGGG AAGAGTTCAG AGAACATTTG GAGCTCATTG TGAAAAATAG TGCAACCTAT 
AATGGTCCGA AGCACTCATT GACCCAGATC TCTCAATCTA TGCTGGATCT CTGTGATGAA 
AAACTCAAAG AGAAAGAAGA TAAATTGGCT CGTTTAGAGA AAGCTATCAA CCCCTTGCTG 
GATGATGATG ACCAAGTAGC ATTTTCTTTC ATTCTGGACA ACATTGTCAC CCAGAAAATG 
ATGGCAGTTC CAGATTCTTG GCCATTTCAT CACCCAGTTA ATAAGAAGTT TGTTCCAGAT 
TATTACAAAG TGATTATCAG TCCAATGGAT TTAGAGACTA TACGTAAGAA TATCTCCAAG 
CACAAGTACC AGAGTCGGGA GAGCTTTCTG GATGATGTCA ACCTTATTCT GGCCAACAGT 
GTTAAGTATA ATGGGCCTGA AAGTCAGTAT ACTAAGACTG CTCAGGAGAT TGTAAATGTC 
TGTTACCAGA CATTGACTGA GTATGATGAA CATTTGACTC AACTTGAGAA GGATATTTGT 
ACAGCTAAGG AAGCGGCTTT GGAGGAAGCA GAATTAGAAA GCTTGGACCC AATGACCCCA 
GGGCCTTATA CACCTCAGCC TCCTGATTTG TATGACACCA ACACATCCCT CAGTATGTCC 
CGAGATGCCT CTGTATTTCA AGATGAGAGC AATATGTCTG TCCTGGATAT TCCCACTGCC 
ACTCCAGAAA AGCAGGTGAC ACAGGAAGGT GAAGATGCAG ATGGTGATCT TGCAGATGAA 
GAGGAAGGAA GTATGCAACA GCCTCAAGCC AGTGTCCTGT ATGAGGATTT GCTTATGTCT 
GAAGGAGAAG ATGATGAAGA AGATGCTGGG AGTGATGAAG AAGGAGACAA TCCTTTCTCT 
GCTATCCAAC TGAGTGAAAG TGGAAGTGAC TCTGATGTTG GATCTAGTGG GATAAGACCC 
AAACAGCCCC GCATGCTTCA GGAAAACACA AGGATGGGCA TGGAAAATGA AGAAAGCATG 
ATGTCCTATG AGGGAGATGG TGGGGAGGCT TCTCATGGTT TGGAGGATAG CAATATGAGT 
TATGGGAGCT ATGAGGAGCC TGATCCCAAG TCGAACACCC AAGATACAAG CTTCAGCAGC 
ATCGGTGGGT ATGAGGTATC AGAGGAAGAA GAAGATGAGG AGGAAGAGCA GCGCTCTGGG 
CCAAGTGTAC TAAGCCAGGT CCACCTGTCA GAGGATGAGG AGGACAGTGA GGATTTCCAC 
TCCATTGCTG GGGACAGTGA TTTGGACTCT GATGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003435609.1
CDS	1..5679
Translation	MGPGWDLLLPVAAGDTTSAIMSDTDSDEDSAGGGPFSLTGFLFGNINGAGQLEGESVLDDECKKHLAGLGALGLGSLITELTANEELAGTDGALVNDEGWIRSTEDAVDYSDITEVAEDESRRYQQTMGSLQPLCHSDYDEDDYDADCEDIDCKLMPPPPPPPGPMKKDKDQDGLTGVSEDGEGIILPSIIAPSSLASEKVDFSSSSDSESEMGPQEASQAESEDGKLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSARRKRKKKHRELIQEEQIQEVECSVESEVNKKSLWNYDYAPPPPPEQCLSDDEITMMAPVESKFSQSTGDIDKVTDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKLRKMDHEPVIKCRMMEDLRKFEENNGSDLLADENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDKPWYSIFPIDNEELVYGRWEDNIIWDAQAMPRLLEPPVLTLDPNDENLILEIPDEKEEATSNSPSKENKKESSLKKSRILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYYIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYYSMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEILSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMLLAAGSAASGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKPAVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIISPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNGPESQYTKTAQEIVNVCYQTLTEYDEHLTQLEKDICTAKEAALEEAELESLDPMTPGPYTPQPPDLYDTNTSLSMSRDASVFQDESNMSVLDIPTATPEKQVTQEGEDADGDLADEEEGSMQQPQASVLYEDLLMSEGEDDEEDAGSDEEGDNPFSAIQLSESGSDSDVGSSGIRPKQPRMLQENTRMGMENEESMMSYEGDGGEASHGLEDSNMSYGSYEEPDPKSNTQDTSFSSIGGYEVSEEEEDEEEEQRSGPSVLSQVHLSEDEEDSEDFHSIAGDSDLDSDE

Target ORF information:

RefSeq Version	XM_003435561.4
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris TATA-box binding protein associated factor 1 (TAF1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003435561.4

ORF Insert Sequence:

ATGGGACCTG GCTGGGATTT GCTGCTGCCT GTAGCCGCCG GAGACACTAC CAGCGCCATC 
ATGTCAGACA CGGACAGCGA TGAAGATTCT GCTGGAGGCG GCCCATTTTC TCTAACCGGT 
TTCCTTTTTG GCAACATCAA TGGAGCCGGG CAGCTGGAGG GGGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGTC TGGGCAGCCT GATCACTGAA 
CTCACGGCAA ACGAAGAATT GGCTGGGACC GATGGTGCCC TGGTAAATGA TGAAGGATGG 
ATCAGGAGTA CAGAAGATGC TGTGGACTAT TCAGACATCA CTGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ATCAGCAGAC AATGGGGAGC TTGCAGCCCC TTTGCCACTC AGATTATGAT 
GAAGATGACT ATGATGCCGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAATGAA GAAAGATAAG GACCAGGATG GTCTTACCGG TGTGTCTGAG 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCATCACAG 
GCAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA ACACGATGCC 
ACCAAGCTGT TGCCAAGTGT CACAGAACTT TTCCCGGAAT TTCGGCCTGG GAAGGTATTA 
CGCTTCCTTC GTCTTTTCGG ACCAGGGAAG AATGTCCCAT CTGTTTGGCG GAGTGCTCGG 
AGAAAGAGGA AGAAGAAGCA CCGTGAGCTG ATACAGGAAG AACAGATCCA GGAGGTGGAG 
TGCTCAGTAG AGTCCGAAGT CAACAAGAAG TCTTTATGGA ATTATGACTA TGCTCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATTACAA TGATGGCTCC TGTTGAATCA 
AAGTTTTCCC AGTCAACTGG AGATATAGAT AAAGTGACAG ATACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ATTATGGCTT CAAACTGAGA AAAATGGACC ATGAGCCTGT GATAAAATGT 
AGAATGATGG AGGACTTGAG GAAATTTGAG GAAAACAATG GCTCTGACCT ACTGGCTGAT 
GAAAACTTCC TGATGGTGAC ACAGCTGCAC TGGGAGGATG ATATCATCTG GGATGGGGAG 
GATGTCAAGC ACAAAGGAAC AAAGCCTCAG CGTGCAAGCC TGGCAGGCTG GCTTCCTTCC 
AGCATGACTA GGAATGCCAT GGCCTACAAT GTTCAGCAAG GTTTTGCAGC CACCCTGGAT 
GATGACAAAC CTTGGTACTC CATCTTTCCC ATTGACAACG AGGAGCTGGT ATATGGACGC 
TGGGAGGACA ATATCATTTG GGATGCTCAG GCCATGCCCA GGCTGTTGGA GCCTCCTGTT 
TTGACACTTG ATCCCAATGA TGAGAACCTT ATTTTGGAAA TTCCTGATGA AAAGGAAGAG 
GCCACTTCTA ACTCCCCCTC CAAGGAAAAT AAGAAAGAAT CATCTCTGAA GAAGAGCCGA 
ATTCTCTTAG GGAAAACAGG GGTCATCAAG GAGGAACCAC AGCAGAACAT GTCTCAGCCA 
GAAGTGAAAG ATCCATGGAA TCTCTCCAAT GATGAATATT ACTACCCCAA GCAACAGGGT 
CTTCGAGGCA CCTTTGGAGG AAATATTATC CAGCACTCAA TTCCTGCAGT GGAATTACGA 
CAGCCCTTCT TTCCTACCCA TATGGGGCCC ATCAAACTTC GGCAGTTCCA TCGTCCACCC 
CTGAAGAAAT ATTCCTTCGG GGCACTCTCT CAGCCAGGTC CCCACTCAGT CCAGCCCTTG 
CTGAAGCACA TCAAAAAGAA GGCTAAGATG AGAGAACAAG AGAGACAAGC TTCTGGTGGT 
GGAGAGATGT TTTTTATGCG CACACCTCAG GACCTCACAG GCAAAGATGG AGATCTTATT 
CTTGCAGAAT ACAGTGAGGA AAATGGACCA TTAATGATGC AGGTTGGCAT GGCAACCAAG 
ATAAAAAACT ATTATAAGCG GAAACCTGGA AAAGATCCTG GGGCCCCAGA TTGTAAATAT 
GGAGAAACTG TTTACTGTCA TACATCTCCT TTCCTAGGCT CTCTTCATCC TGGTCAATTA 
CTGCAGGCAT TTGAGAACAA TCTCTTTCGT GCCCCAATTT ATCTTCATAA GATGCCAGAA 
ACTGACTTCC TTATTATTCG GACAAGACAA GGTTACTACA TTCGGGAGTT AGTGGATATT 
TTTGTGGTCG GCCAGCAATG CCCCTTGTTT GAAGTTCCTG GGCCCAACTC CAAAAGAGCT 
AATACACATA TTCGAGACTT TCTCCAGGTT TTTATTTACC GCCTCTTCTG GAAGAGTAAA 
GATCGGCCAA GGCGGATCCG AATGGAAGAT ATAAAAAAAG CCTTTCCTTC TCACTCAGAA 
AGTAGCATCC GCAAGAGGCT AAAGCTCTGC GCTGACTTCA AACGCACAGG GATGGACTCA 
AACTGGTGGG TGCTAAAGTC AGATTTTCGT TTACCAACTG AAGAGGAGAT CAGAGCTATG 
GTGTCTCCAG AGCAGTGCTG TGCTTATTAT AGCATGATAG CTGCAGAGCA GCGACTAAAG 
GATGCTGGCT ATGGTGAGAA GTCCTTTTTT GCTCCAGAAG AAGAAAATGA GGAAGATTTC 
CAGATGAAGA TTGATGATGA AGTTCGCACT GCTCCTTGGA ATACCACAAG GGCTTTCATT 
GCTGCCATGA AGGGCAAGTG TCTCTTGGAG GTGACTGGGG TGGCAGATCC CACAGGGTGT 
GGTGAAGGAT TCTCCTATGT CAAGATTCCA AACAAACCAA CACAGCAGAA GGATGATAAG 
GAGCCTCAGC CAGTGAAGAA GACAGTGACA GGAACAGATG CAGATCTCCG TCGCCTCTCA 
CTGAAAAATG CCAAGCAGCT CCTACGTAAA TTTGGTGTGC CTGAGGAAGA GATTAAAAAG 
CTGTCCCGCT GGGAAGTGAT TGATGTGGTA CGCACAATGT CAACAGAGCA AGCCCGCTCT 
GGAGAGGGGC CCATGAGTAA ATTTGCCCGT GGATCAAGGT TTTCTGTGGC TGAGCATCAA 
GAGCGTTACA AAGAGGAATG TCAGCGCATC TTTGATCTAC AGAACAAGGT TTTGTCATCA 
ACTGAAATAT TGTCAACTGA CACAGACAGC AGCTCAGCCG AAGACAGTGA CTTTGAAGAA 
ATGGGAAAGA ACATTGAGAA CATGTTGCAG AATAAGAAAA CCAGCTCTCA GCTGTCACGT 
GAACGAGAGG AGCAGGAGCG GAAGGAACTG CAGCGGATGC TACTGGCAGC AGGTTCTGCA 
GCATCAGGAA ACAATCACAG AGATGATGAT ACAGCTTCTG TGACCAGCCT TAACTCTTCT 
GCCACTGGCC GTTGTCTCAA AATTTATCGC ACATTTCGAG ATGAAGAAGG GAAAGAGTAT 
GTTCGCTGTG AGACAGTCCG AAAGCCTGCT GTCATTGATG CCTATGTGCG CATACGGACC 
ACAAAAGATG AGGAATTCAT TCGAAAGTTT GCCCTTTTTG ATGAACAGCA TCGAGAAGAG 
ATGCGAAAGG AACGGCGGAG GATTCAAGAG CAACTTAGGC GGCTCAAGCG GAATCAGGAA 
AAGGAGAAGC TTAAGGGTCC CCCTGAGAAG AAGCCTAAAA AAATGAAGGA GCGTCCTGAT 
CTAAAACTGA AATGTGGAGC TTGTGGTGCC ATTGGGCACA TGAGGACAAA CAAGTTCTGC 
CCCCTCTACT ATCAAACAAA TGCTCCACCT TCCAACCCTG TTGCCATGAC AGAGGAGCAG 
GAGGAGGAGT TGGAAAAGAC AGTCATTCAT AATGATAATG AAGAACTTAT CAAGGTTGAA 
GGGACCAAGA TCGTCCTGGG AAAACAGCTA ATTGAGAGTG CAGATGAGGT TCGAAGAAAA 
TCTCTGGTTC TCAAGTTCCC TAAACAGCAA CTTCCTCCCA AGAAGAAACG GCGAGTTGGA 
ACCACTGTTC ACTGTGACTA CTTAAATAGA CCTCATAAAT CCATCCACCG GCGCCGGACA 
GACCCAATGG TGACATTGTC CTCCATCTTG GAGTCTATCA TCAATGACAT GAGAGATCTT 
CCAAATACCT ACCCTTTCCA CACTCCAGTC AATGCAAAGG TTGTAAAGGA CTACTACAAA 
ATCATCACTC GACCAATGGA CTTACAAACA CTCCGTGAAA ATGTGCGCAA ACGCCTTTAC 
CCATCTCGGG AAGAGTTCAG AGAACATTTG GAGCTCATTG TGAAAAATAG TGCAACCTAT 
AATGGTCCGA AGCACTCATT GACCCAGATC TCTCAATCTA TGCTGGATCT CTGTGATGAA 
AAACTCAAAG AGAAAGAAGA TAAATTGGCT CGTTTAGAGA AAGCTATCAA CCCCTTGCTG 
GATGATGATG ACCAAGTAGC ATTTTCTTTC ATTCTGGACA ACATTGTCAC CCAGAAAATG 
ATGGCAGTTC CAGATTCTTG GCCATTTCAT CACCCAGTTA ATAAGAAGTT TGTTCCAGAT 
TATTACAAAG TGATTATCAG TCCAATGGAT TTAGAGACTA TACGTAAGAA TATCTCCAAG 
CACAAGTACC AGAGTCGGGA GAGCTTTCTG GATGATGTCA ACCTTATTCT GGCCAACAGT 
GTTAAGTATA ATGGGCCTGA AAGTCAGTAT ACTAAGACTG CTCAGGAGAT TGTAAATGTC 
TGTTACCAGA CATTGACTGA GTATGATGAA CATTTGACTC AACTTGAGAA GGATATTTGT 
ACAGCTAAGG AAGCGGCTTT GGAGGAAGCA GAATTAGAAA GCTTGGACCC AATGACCCCA 
GGGCCTTATA CACCTCAGCC TCCTGATTTG TATGACACCA ACACATCCCT CAGTATGTCC 
CGAGATGCCT CTGTATTTCA AGATGAGAGC AATATGTCTG TCCTGGATAT TCCCACTGCC 
ACTCCAGAAA AGCAGGTGAC ACAGGAAGGT GAAGATGCAG ATGGTGATCT TGCAGATGAA 
GAGGAAGGAA GTATGCAACA GCCTCAAGCC AGTGTCCTGT ATGAGGATTT GCTTATGTCT 
GAAGGAGAAG ATGATGAAGA AGATGCTGGG AGTGATGAAG AAGGAGACAA TCCTTTCTCT 
GCTATCCAAC TGAGTGAAAG TGGAAGTGAC TCTGATGTTG GATCTAGTGG GATAAGACCC 
AAACAGCCCC GCATGCTTCA GGAAAACACA AGGATGGGCA TGGAAAATGA AGAAAGCATG 
ATGTCCTATG AGGGAGATGG TGGGGAGGCT TCTCATGGTT TGGAGGATAG CAATATGAGT 
TATGGGAGCT ATGAGGAGCC TGATCCCAAG TCGAACACCC AAGATACAAG CTTCAGCAGC 
ATCGGTGGGT ATGAGGTATC AGAGGAAGAA GAAGATGAGG AGGAAGAGCA GCGCTCTGGG 
CCAAGTGTAC TAAGCCAGGT CCACCTGTCA GAGGATGAGG AGGACAGTGA GGATTTCCAC 
TCCATTGCTG GGGACAGTGA TTTGGACTCT GATGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAF1 ( XM_003435561.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003435609.1 Canis lupus familiaris TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa (TAF1), transcript variant X6, mRNA.

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CloneID	OCe32532
Clone ID Related Accession (Same CDS sequence)	XM_003435561.4 , XM_003435561.3
Accession Version	XM_003435561.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5679bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	transcription initiation factor TFIID subunit 1 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003435561.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACCTG GCTGGGATTT GCTGCTGCCT GTAGCCGCCG GAGACACTAC CAGCGCCATC 
ATGTCAGACA CGGACAGCGA TGAAGATTCT GCTGGAGGCG GCCCATTTTC TCTAACCGGT 
TTCCTTTTTG GCAACATCAA TGGAGCCGGG CAGCTGGAGG GGGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGTC TGGGCAGCCT GATCACTGAA 
CTCACGGCAA ACGAAGAATT GGCTGGGACC GATGGTGCCC TGGTAAATGA TGAAGGATGG 
ATCAGGAGTA CAGAAGATGC TGTGGACTAT TCAGACATCA CTGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ATCAGCAGAC AATGGGGAGC TTGCAGCCCC TTTGCCACTC AGATTATGAT 
GAAGATGACT ATGATGCCGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAATGAA GAAAGATAAG GACCAGGATG GTCTTACCGG TGTGTCTGAG 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCATCACAG 
GCAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA ACACGATGCC 
ACCAAGCTGT TGCCAAGTGT CACAGAACTT TTCCCGGAAT TTCGGCCTGG GAAGGTATTA 
CGCTTCCTTC GTCTTTTCGG ACCAGGGAAG AATGTCCCAT CTGTTTGGCG GAGTGCTCGG 
AGAAAGAGGA AGAAGAAGCA CCGTGAGCTG ATACAGGAAG AACAGATCCA GGAGGTGGAG 
TGCTCAGTAG AGTCCGAAGT CAACAAGAAG TCTTTATGGA ATTATGACTA TGCTCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATTACAA TGATGGCTCC TGTTGAATCA 
AAGTTTTCCC AGTCAACTGG AGATATAGAT AAAGTGACAG ATACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ATTATGGCTT CAAACTGAGA AAAATGGACC ATGAGCCTGT GATAAAATGT 
AGAATGATGG AGGACTTGAG GAAATTTGAG GAAAACAATG GCTCTGACCT ACTGGCTGAT 
GAAAACTTCC TGATGGTGAC ACAGCTGCAC TGGGAGGATG ATATCATCTG GGATGGGGAG 
GATGTCAAGC ACAAAGGAAC AAAGCCTCAG CGTGCAAGCC TGGCAGGCTG GCTTCCTTCC 
AGCATGACTA GGAATGCCAT GGCCTACAAT GTTCAGCAAG GTTTTGCAGC CACCCTGGAT 
GATGACAAAC CTTGGTACTC CATCTTTCCC ATTGACAACG AGGAGCTGGT ATATGGACGC 
TGGGAGGACA ATATCATTTG GGATGCTCAG GCCATGCCCA GGCTGTTGGA GCCTCCTGTT 
TTGACACTTG ATCCCAATGA TGAGAACCTT ATTTTGGAAA TTCCTGATGA AAAGGAAGAG 
GCCACTTCTA ACTCCCCCTC CAAGGAAAAT AAGAAAGAAT CATCTCTGAA GAAGAGCCGA 
ATTCTCTTAG GGAAAACAGG GGTCATCAAG GAGGAACCAC AGCAGAACAT GTCTCAGCCA 
GAAGTGAAAG ATCCATGGAA TCTCTCCAAT GATGAATATT ACTACCCCAA GCAACAGGGT 
CTTCGAGGCA CCTTTGGAGG AAATATTATC CAGCACTCAA TTCCTGCAGT GGAATTACGA 
CAGCCCTTCT TTCCTACCCA TATGGGGCCC ATCAAACTTC GGCAGTTCCA TCGTCCACCC 
CTGAAGAAAT ATTCCTTCGG GGCACTCTCT CAGCCAGGTC CCCACTCAGT CCAGCCCTTG 
CTGAAGCACA TCAAAAAGAA GGCTAAGATG AGAGAACAAG AGAGACAAGC TTCTGGTGGT 
GGAGAGATGT TTTTTATGCG CACACCTCAG GACCTCACAG GCAAAGATGG AGATCTTATT 
CTTGCAGAAT ACAGTGAGGA AAATGGACCA TTAATGATGC AGGTTGGCAT GGCAACCAAG 
ATAAAAAACT ATTATAAGCG GAAACCTGGA AAAGATCCTG GGGCCCCAGA TTGTAAATAT 
GGAGAAACTG TTTACTGTCA TACATCTCCT TTCCTAGGCT CTCTTCATCC TGGTCAATTA 
CTGCAGGCAT TTGAGAACAA TCTCTTTCGT GCCCCAATTT ATCTTCATAA GATGCCAGAA 
ACTGACTTCC TTATTATTCG GACAAGACAA GGTTACTACA TTCGGGAGTT AGTGGATATT 
TTTGTGGTCG GCCAGCAATG CCCCTTGTTT GAAGTTCCTG GGCCCAACTC CAAAAGAGCT 
AATACACATA TTCGAGACTT TCTCCAGGTT TTTATTTACC GCCTCTTCTG GAAGAGTAAA 
GATCGGCCAA GGCGGATCCG AATGGAAGAT ATAAAAAAAG CCTTTCCTTC TCACTCAGAA 
AGTAGCATCC GCAAGAGGCT AAAGCTCTGC GCTGACTTCA AACGCACAGG GATGGACTCA 
AACTGGTGGG TGCTAAAGTC AGATTTTCGT TTACCAACTG AAGAGGAGAT CAGAGCTATG 
GTGTCTCCAG AGCAGTGCTG TGCTTATTAT AGCATGATAG CTGCAGAGCA GCGACTAAAG 
GATGCTGGCT ATGGTGAGAA GTCCTTTTTT GCTCCAGAAG AAGAAAATGA GGAAGATTTC 
CAGATGAAGA TTGATGATGA AGTTCGCACT GCTCCTTGGA ATACCACAAG GGCTTTCATT 
GCTGCCATGA AGGGCAAGTG TCTCTTGGAG GTGACTGGGG TGGCAGATCC CACAGGGTGT 
GGTGAAGGAT TCTCCTATGT CAAGATTCCA AACAAACCAA CACAGCAGAA GGATGATAAG 
GAGCCTCAGC CAGTGAAGAA GACAGTGACA GGAACAGATG CAGATCTCCG TCGCCTCTCA 
CTGAAAAATG CCAAGCAGCT CCTACGTAAA TTTGGTGTGC CTGAGGAAGA GATTAAAAAG 
CTGTCCCGCT GGGAAGTGAT TGATGTGGTA CGCACAATGT CAACAGAGCA AGCCCGCTCT 
GGAGAGGGGC CCATGAGTAA ATTTGCCCGT GGATCAAGGT TTTCTGTGGC TGAGCATCAA 
GAGCGTTACA AAGAGGAATG TCAGCGCATC TTTGATCTAC AGAACAAGGT TTTGTCATCA 
ACTGAAATAT TGTCAACTGA CACAGACAGC AGCTCAGCCG AAGACAGTGA CTTTGAAGAA 
ATGGGAAAGA ACATTGAGAA CATGTTGCAG AATAAGAAAA CCAGCTCTCA GCTGTCACGT 
GAACGAGAGG AGCAGGAGCG GAAGGAACTG CAGCGGATGC TACTGGCAGC AGGTTCTGCA 
GCATCAGGAA ACAATCACAG AGATGATGAT ACAGCTTCTG TGACCAGCCT TAACTCTTCT 
GCCACTGGCC GTTGTCTCAA AATTTATCGC ACATTTCGAG ATGAAGAAGG GAAAGAGTAT 
GTTCGCTGTG AGACAGTCCG AAAGCCTGCT GTCATTGATG CCTATGTGCG CATACGGACC 
ACAAAAGATG AGGAATTCAT TCGAAAGTTT GCCCTTTTTG ATGAACAGCA TCGAGAAGAG 
ATGCGAAAGG AACGGCGGAG GATTCAAGAG CAACTTAGGC GGCTCAAGCG GAATCAGGAA 
AAGGAGAAGC TTAAGGGTCC CCCTGAGAAG AAGCCTAAAA AAATGAAGGA GCGTCCTGAT 
CTAAAACTGA AATGTGGAGC TTGTGGTGCC ATTGGGCACA TGAGGACAAA CAAGTTCTGC 
CCCCTCTACT ATCAAACAAA TGCTCCACCT TCCAACCCTG TTGCCATGAC AGAGGAGCAG 
GAGGAGGAGT TGGAAAAGAC AGTCATTCAT AATGATAATG AAGAACTTAT CAAGGTTGAA 
GGGACCAAGA TCGTCCTGGG AAAACAGCTA ATTGAGAGTG CAGATGAGGT TCGAAGAAAA 
TCTCTGGTTC TCAAGTTCCC TAAACAGCAA CTTCCTCCCA AGAAGAAACG GCGAGTTGGA 
ACCACTGTTC ACTGTGACTA CTTAAATAGA CCTCATAAAT CCATCCACCG GCGCCGGACA 
GACCCAATGG TGACATTGTC CTCCATCTTG GAGTCTATCA TCAATGACAT GAGAGATCTT 
CCAAATACCT ACCCTTTCCA CACTCCAGTC AATGCAAAGG TTGTAAAGGA CTACTACAAA 
ATCATCACTC GACCAATGGA CTTACAAACA CTCCGTGAAA ATGTGCGCAA ACGCCTTTAC 
CCATCTCGGG AAGAGTTCAG AGAACATTTG GAGCTCATTG TGAAAAATAG TGCAACCTAT 
AATGGTCCGA AGCACTCATT GACCCAGATC TCTCAATCTA TGCTGGATCT CTGTGATGAA 
AAACTCAAAG AGAAAGAAGA TAAATTGGCT CGTTTAGAGA AAGCTATCAA CCCCTTGCTG 
GATGATGATG ACCAAGTAGC ATTTTCTTTC ATTCTGGACA ACATTGTCAC CCAGAAAATG 
ATGGCAGTTC CAGATTCTTG GCCATTTCAT CACCCAGTTA ATAAGAAGTT TGTTCCAGAT 
TATTACAAAG TGATTATCAG TCCAATGGAT TTAGAGACTA TACGTAAGAA TATCTCCAAG 
CACAAGTACC AGAGTCGGGA GAGCTTTCTG GATGATGTCA ACCTTATTCT GGCCAACAGT 
GTTAAGTATA ATGGGCCTGA AAGTCAGTAT ACTAAGACTG CTCAGGAGAT TGTAAATGTC 
TGTTACCAGA CATTGACTGA GTATGATGAA CATTTGACTC AACTTGAGAA GGATATTTGT 
ACAGCTAAGG AAGCGGCTTT GGAGGAAGCA GAATTAGAAA GCTTGGACCC AATGACCCCA 
GGGCCTTATA CACCTCAGCC TCCTGATTTG TATGACACCA ACACATCCCT CAGTATGTCC 
CGAGATGCCT CTGTATTTCA AGATGAGAGC AATATGTCTG TCCTGGATAT TCCCACTGCC 
ACTCCAGAAA AGCAGGTGAC ACAGGAAGGT GAAGATGCAG ATGGTGATCT TGCAGATGAA 
GAGGAAGGAA GTATGCAACA GCCTCAAGCC AGTGTCCTGT ATGAGGATTT GCTTATGTCT 
GAAGGAGAAG ATGATGAAGA AGATGCTGGG AGTGATGAAG AAGGAGACAA TCCTTTCTCT 
GCTATCCAAC TGAGTGAAAG TGGAAGTGAC TCTGATGTTG GATCTAGTGG GATAAGACCC 
AAACAGCCCC GCATGCTTCA GGAAAACACA AGGATGGGCA TGGAAAATGA AGAAAGCATG 
ATGTCCTATG AGGGAGATGG TGGGGAGGCT TCTCATGGTT TGGAGGATAG CAATATGAGT 
TATGGGAGCT ATGAGGAGCC TGATCCCAAG TCGAACACCC AAGATACAAG CTTCAGCAGC 
ATCGGTGGGT ATGAGGTATC AGAGGAAGAA GAAGATGAGG AGGAAGAGCA GCGCTCTGGG 
CCAAGTGTAC TAAGCCAGGT CCACCTGTCA GAGGATGAGG AGGACAGTGA GGATTTCCAC 
TCCATTGCTG GGGACAGTGA TTTGGACTCT GATGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003435609.1
CDS	93..5771
Translation	MGPGWDLLLPVAAGDTTSAIMSDTDSDEDSAGGGPFSLTGFLFGNINGAGQLEGESVLDDECKKHLAGLGALGLGSLITELTANEELAGTDGALVNDEGWIRSTEDAVDYSDITEVAEDESRRYQQTMGSLQPLCHSDYDEDDYDADCEDIDCKLMPPPPPPPGPMKKDKDQDGLTGVSEDGEGIILPSIIAPSSLASEKVDFSSSSDSESEMGPQEASQAESEDGKLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSARRKRKKKHRELIQEEQIQEVECSVESEVNKKSLWNYDYAPPPPPEQCLSDDEITMMAPVESKFSQSTGDIDKVTDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKLRKMDHEPVIKCRMMEDLRKFEENNGSDLLADENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDKPWYSIFPIDNEELVYGRWEDNIIWDAQAMPRLLEPPVLTLDPNDENLILEIPDEKEEATSNSPSKENKKESSLKKSRILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYYIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYYSMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEILSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMLLAAGSAASGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKPAVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIISPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNGPESQYTKTAQEIVNVCYQTLTEYDEHLTQLEKDICTAKEAALEEAELESLDPMTPGPYTPQPPDLYDTNTSLSMSRDASVFQDESNMSVLDIPTATPEKQVTQEGEDADGDLADEEEGSMQQPQASVLYEDLLMSEGEDDEEDAGSDEEGDNPFSAIQLSESGSDSDVGSSGIRPKQPRMLQENTRMGMENEESMMSYEGDGGEASHGLEDSNMSYGSYEEPDPKSNTQDTSFSSIGGYEVSEEEEDEEEEQRSGPSVLSQVHLSEDEEDSEDFHSIAGDSDLDSDE

Target ORF information:

RefSeq Version	XM_003435561.3
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa (TAF1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003435561.3

ORF Insert Sequence:

ATGGGACCTG GCTGGGATTT GCTGCTGCCT GTAGCCGCCG GAGACACTAC CAGCGCCATC 
ATGTCAGACA CGGACAGCGA TGAAGATTCT GCTGGAGGCG GCCCATTTTC TCTAACCGGT 
TTCCTTTTTG GCAACATCAA TGGAGCCGGG CAGCTGGAGG GGGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGTC TGGGCAGCCT GATCACTGAA 
CTCACGGCAA ACGAAGAATT GGCTGGGACC GATGGTGCCC TGGTAAATGA TGAAGGATGG 
ATCAGGAGTA CAGAAGATGC TGTGGACTAT TCAGACATCA CTGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ATCAGCAGAC AATGGGGAGC TTGCAGCCCC TTTGCCACTC AGATTATGAT 
GAAGATGACT ATGATGCCGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAATGAA GAAAGATAAG GACCAGGATG GTCTTACCGG TGTGTCTGAG 
GATGGAGAAG GCATCATCTT GCCCTCCATC ATTGCCCCTT CCTCTTTGGC CTCAGAGAAA 
GTGGACTTCA GTAGTTCCTC TGACTCAGAA TCTGAGATGG GACCTCAGGA AGCATCACAG 
GCAGAATCTG AGGATGGAAA GCTGACCCTA CCATTGGCTG GGATTATGCA ACACGATGCC 
ACCAAGCTGT TGCCAAGTGT CACAGAACTT TTCCCGGAAT TTCGGCCTGG GAAGGTATTA 
CGCTTCCTTC GTCTTTTCGG ACCAGGGAAG AATGTCCCAT CTGTTTGGCG GAGTGCTCGG 
AGAAAGAGGA AGAAGAAGCA CCGTGAGCTG ATACAGGAAG AACAGATCCA GGAGGTGGAG 
TGCTCAGTAG AGTCCGAAGT CAACAAGAAG TCTTTATGGA ATTATGACTA TGCTCCACCA 
CCACCTCCAG AGCAGTGTCT CTCTGATGAT GAAATTACAA TGATGGCTCC TGTTGAATCA 
AAGTTTTCCC AGTCAACTGG AGATATAGAT AAAGTGACAG ATACCAAACC AAGAGTGGCT 
GAGTGGCGTT ATGGGCCTGC TCGACTGTGG TATGATATGC TGGGTGTCCC TGAAGATGGC 
AGTGGGTTTG ATTATGGCTT CAAACTGAGA AAAATGGACC ATGAGCCTGT GATAAAATGT 
AGAATGATGG AGGACTTGAG GAAATTTGAG GAAAACAATG GCTCTGACCT ACTGGCTGAT 
GAAAACTTCC TGATGGTGAC ACAGCTGCAC TGGGAGGATG ATATCATCTG GGATGGGGAG 
GATGTCAAGC ACAAAGGAAC AAAGCCTCAG CGTGCAAGCC TGGCAGGCTG GCTTCCTTCC 
AGCATGACTA GGAATGCCAT GGCCTACAAT GTTCAGCAAG GTTTTGCAGC CACCCTGGAT 
GATGACAAAC CTTGGTACTC CATCTTTCCC ATTGACAACG AGGAGCTGGT ATATGGACGC 
TGGGAGGACA ATATCATTTG GGATGCTCAG GCCATGCCCA GGCTGTTGGA GCCTCCTGTT 
TTGACACTTG ATCCCAATGA TGAGAACCTT ATTTTGGAAA TTCCTGATGA AAAGGAAGAG 
GCCACTTCTA ACTCCCCCTC CAAGGAAAAT AAGAAAGAAT CATCTCTGAA GAAGAGCCGA 
ATTCTCTTAG GGAAAACAGG GGTCATCAAG GAGGAACCAC AGCAGAACAT GTCTCAGCCA 
GAAGTGAAAG ATCCATGGAA TCTCTCCAAT GATGAATATT ACTACCCCAA GCAACAGGGT 
CTTCGAGGCA CCTTTGGAGG AAATATTATC CAGCACTCAA TTCCTGCAGT GGAATTACGA 
CAGCCCTTCT TTCCTACCCA TATGGGGCCC ATCAAACTTC GGCAGTTCCA TCGTCCACCC 
CTGAAGAAAT ATTCCTTCGG GGCACTCTCT CAGCCAGGTC CCCACTCAGT CCAGCCCTTG 
CTGAAGCACA TCAAAAAGAA GGCTAAGATG AGAGAACAAG AGAGACAAGC TTCTGGTGGT 
GGAGAGATGT TTTTTATGCG CACACCTCAG GACCTCACAG GCAAAGATGG AGATCTTATT 
CTTGCAGAAT ACAGTGAGGA AAATGGACCA TTAATGATGC AGGTTGGCAT GGCAACCAAG 
ATAAAAAACT ATTATAAGCG GAAACCTGGA AAAGATCCTG GGGCCCCAGA TTGTAAATAT 
GGAGAAACTG TTTACTGTCA TACATCTCCT TTCCTAGGCT CTCTTCATCC TGGTCAATTA 
CTGCAGGCAT TTGAGAACAA TCTCTTTCGT GCCCCAATTT ATCTTCATAA GATGCCAGAA 
ACTGACTTCC TTATTATTCG GACAAGACAA GGTTACTACA TTCGGGAGTT AGTGGATATT 
TTTGTGGTCG GCCAGCAATG CCCCTTGTTT GAAGTTCCTG GGCCCAACTC CAAAAGAGCT 
AATACACATA TTCGAGACTT TCTCCAGGTT TTTATTTACC GCCTCTTCTG GAAGAGTAAA 
GATCGGCCAA GGCGGATCCG AATGGAAGAT ATAAAAAAAG CCTTTCCTTC TCACTCAGAA 
AGTAGCATCC GCAAGAGGCT AAAGCTCTGC GCTGACTTCA AACGCACAGG GATGGACTCA 
AACTGGTGGG TGCTAAAGTC AGATTTTCGT TTACCAACTG AAGAGGAGAT CAGAGCTATG 
GTGTCTCCAG AGCAGTGCTG TGCTTATTAT AGCATGATAG CTGCAGAGCA GCGACTAAAG 
GATGCTGGCT ATGGTGAGAA GTCCTTTTTT GCTCCAGAAG AAGAAAATGA GGAAGATTTC 
CAGATGAAGA TTGATGATGA AGTTCGCACT GCTCCTTGGA ATACCACAAG GGCTTTCATT 
GCTGCCATGA AGGGCAAGTG TCTCTTGGAG GTGACTGGGG TGGCAGATCC CACAGGGTGT 
GGTGAAGGAT TCTCCTATGT CAAGATTCCA AACAAACCAA CACAGCAGAA GGATGATAAG 
GAGCCTCAGC CAGTGAAGAA GACAGTGACA GGAACAGATG CAGATCTCCG TCGCCTCTCA 
CTGAAAAATG CCAAGCAGCT CCTACGTAAA TTTGGTGTGC CTGAGGAAGA GATTAAAAAG 
CTGTCCCGCT GGGAAGTGAT TGATGTGGTA CGCACAATGT CAACAGAGCA AGCCCGCTCT 
GGAGAGGGGC CCATGAGTAA ATTTGCCCGT GGATCAAGGT TTTCTGTGGC TGAGCATCAA 
GAGCGTTACA AAGAGGAATG TCAGCGCATC TTTGATCTAC AGAACAAGGT TTTGTCATCA 
ACTGAAATAT TGTCAACTGA CACAGACAGC AGCTCAGCCG AAGACAGTGA CTTTGAAGAA 
ATGGGAAAGA ACATTGAGAA CATGTTGCAG AATAAGAAAA CCAGCTCTCA GCTGTCACGT 
GAACGAGAGG AGCAGGAGCG GAAGGAACTG CAGCGGATGC TACTGGCAGC AGGTTCTGCA 
GCATCAGGAA ACAATCACAG AGATGATGAT ACAGCTTCTG TGACCAGCCT TAACTCTTCT 
GCCACTGGCC GTTGTCTCAA AATTTATCGC ACATTTCGAG ATGAAGAAGG GAAAGAGTAT 
GTTCGCTGTG AGACAGTCCG AAAGCCTGCT GTCATTGATG CCTATGTGCG CATACGGACC 
ACAAAAGATG AGGAATTCAT TCGAAAGTTT GCCCTTTTTG ATGAACAGCA TCGAGAAGAG 
ATGCGAAAGG AACGGCGGAG GATTCAAGAG CAACTTAGGC GGCTCAAGCG GAATCAGGAA 
AAGGAGAAGC TTAAGGGTCC CCCTGAGAAG AAGCCTAAAA AAATGAAGGA GCGTCCTGAT 
CTAAAACTGA AATGTGGAGC TTGTGGTGCC ATTGGGCACA TGAGGACAAA CAAGTTCTGC 
CCCCTCTACT ATCAAACAAA TGCTCCACCT TCCAACCCTG TTGCCATGAC AGAGGAGCAG 
GAGGAGGAGT TGGAAAAGAC AGTCATTCAT AATGATAATG AAGAACTTAT CAAGGTTGAA 
GGGACCAAGA TCGTCCTGGG AAAACAGCTA ATTGAGAGTG CAGATGAGGT TCGAAGAAAA 
TCTCTGGTTC TCAAGTTCCC TAAACAGCAA CTTCCTCCCA AGAAGAAACG GCGAGTTGGA 
ACCACTGTTC ACTGTGACTA CTTAAATAGA CCTCATAAAT CCATCCACCG GCGCCGGACA 
GACCCAATGG TGACATTGTC CTCCATCTTG GAGTCTATCA TCAATGACAT GAGAGATCTT 
CCAAATACCT ACCCTTTCCA CACTCCAGTC AATGCAAAGG TTGTAAAGGA CTACTACAAA 
ATCATCACTC GACCAATGGA CTTACAAACA CTCCGTGAAA ATGTGCGCAA ACGCCTTTAC 
CCATCTCGGG AAGAGTTCAG AGAACATTTG GAGCTCATTG TGAAAAATAG TGCAACCTAT 
AATGGTCCGA AGCACTCATT GACCCAGATC TCTCAATCTA TGCTGGATCT CTGTGATGAA 
AAACTCAAAG AGAAAGAAGA TAAATTGGCT CGTTTAGAGA AAGCTATCAA CCCCTTGCTG 
GATGATGATG ACCAAGTAGC ATTTTCTTTC ATTCTGGACA ACATTGTCAC CCAGAAAATG 
ATGGCAGTTC CAGATTCTTG GCCATTTCAT CACCCAGTTA ATAAGAAGTT TGTTCCAGAT 
TATTACAAAG TGATTATCAG TCCAATGGAT TTAGAGACTA TACGTAAGAA TATCTCCAAG 
CACAAGTACC AGAGTCGGGA GAGCTTTCTG GATGATGTCA ACCTTATTCT GGCCAACAGT 
GTTAAGTATA ATGGGCCTGA AAGTCAGTAT ACTAAGACTG CTCAGGAGAT TGTAAATGTC 
TGTTACCAGA CATTGACTGA GTATGATGAA CATTTGACTC AACTTGAGAA GGATATTTGT 
ACAGCTAAGG AAGCGGCTTT GGAGGAAGCA GAATTAGAAA GCTTGGACCC AATGACCCCA 
GGGCCTTATA CACCTCAGCC TCCTGATTTG TATGACACCA ACACATCCCT CAGTATGTCC 
CGAGATGCCT CTGTATTTCA AGATGAGAGC AATATGTCTG TCCTGGATAT TCCCACTGCC 
ACTCCAGAAA AGCAGGTGAC ACAGGAAGGT GAAGATGCAG ATGGTGATCT TGCAGATGAA 
GAGGAAGGAA GTATGCAACA GCCTCAAGCC AGTGTCCTGT ATGAGGATTT GCTTATGTCT 
GAAGGAGAAG ATGATGAAGA AGATGCTGGG AGTGATGAAG AAGGAGACAA TCCTTTCTCT 
GCTATCCAAC TGAGTGAAAG TGGAAGTGAC TCTGATGTTG GATCTAGTGG GATAAGACCC 
AAACAGCCCC GCATGCTTCA GGAAAACACA AGGATGGGCA TGGAAAATGA AGAAAGCATG 
ATGTCCTATG AGGGAGATGG TGGGGAGGCT TCTCATGGTT TGGAGGATAG CAATATGAGT 
TATGGGAGCT ATGAGGAGCC TGATCCCAAG TCGAACACCC AAGATACAAG CTTCAGCAGC 
ATCGGTGGGT ATGAGGTATC AGAGGAAGAA GAAGATGAGG AGGAAGAGCA GCGCTCTGGG 
CCAAGTGTAC TAAGCCAGGT CCACCTGTCA GAGGATGAGG AGGACAGTGA GGATTTCCAC 
TCCATTGCTG GGGACAGTGA TTTGGACTCT GATGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAF1 ( XM_844234.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_849327.1 Canis lupus familiaris TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa (TAF1), transcript variant X7, mRNA.

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CloneID	OCe32533
Clone ID Related Accession (Same CDS sequence)	XM_844234.4 , XM_844234.5
Accession Version	XM_844234.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5616bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	transcription initiation factor TFIID subunit 1 isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_844234.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACCTG GCTGGGATTT GCTGCTGCCT GTAGCCGCCG GAGACACTAC CAGCGCCATC 
ATGTCAGACA CGGACAGCGA TGAAGATTCT GCTGGAGGCG GCCCATTTTC TCTAACCGGT 
TTCCTTTTTG GCAACATCAA TGGAGCCGGG CAGCTGGAGG GGGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGTC TGGGCAGCCT GATCACTGAA 
CTCACGGCAA ACGAAGAATT GGCTGGGACC GATGGTGCCC TGGTAAATGA TGAAGGATGG 
ATCAGGAGTA CAGAAGATGC TGTGGACTAT TCAGACATCA CTGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ATCAGCAGAC AATGGGGAGC TTGCAGCCCC TTTGCCACTC AGATTATGAT 
GAAGATGACT ATGATGCCGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAATGAA GAAAGATAAG GACCAGGATG GTCTTACCGG TGAGAAAGTG 
GACTTCAGTA GTTCCTCTGA CTCAGAATCT GAGATGGGAC CTCAGGAAGC ATCACAGGCA 
GAATCTGAGG ATGGAAAGCT GACCCTACCA TTGGCTGGGA TTATGCAACA CGATGCCACC 
AAGCTGTTGC CAAGTGTCAC AGAACTTTTC CCGGAATTTC GGCCTGGGAA GGTATTACGC 
TTCCTTCGTC TTTTCGGACC AGGGAAGAAT GTCCCATCTG TTTGGCGGAG TGCTCGGAGA 
AAGAGGAAGA AGAAGCACCG TGAGCTGATA CAGGAAGAAC AGATCCAGGA GGTGGAGTGC 
TCAGTAGAGT CCGAAGTCAA CAAGAAGTCT TTATGGAATT ATGACTATGC TCCACCACCA 
CCTCCAGAGC AGTGTCTCTC TGATGATGAA ATTACAATGA TGGCTCCTGT TGAATCAAAG 
TTTTCCCAGT CAACTGGAGA TATAGATAAA GTGACAGATA CCAAACCAAG AGTGGCTGAG 
TGGCGTTATG GGCCTGCTCG ACTGTGGTAT GATATGCTGG GTGTCCCTGA AGATGGCAGT 
GGGTTTGATT ATGGCTTCAA ACTGAGAAAA ATGGACCATG AGCCTGTGAT AAAATGTAGA 
ATGATGGAGG ACTTGAGGAA ATTTGAGGAA AACAATGGCT CTGACCTACT GGCTGATGAA 
AACTTCCTGA TGGTGACACA GCTGCACTGG GAGGATGATA TCATCTGGGA TGGGGAGGAT 
GTCAAGCACA AAGGAACAAA GCCTCAGCGT GCAAGCCTGG CAGGCTGGCT TCCTTCCAGC 
ATGACTAGGA ATGCCATGGC CTACAATGTT CAGCAAGGTT TTGCAGCCAC CCTGGATGAT 
GACAAACCTT GGTACTCCAT CTTTCCCATT GACAACGAGG AGCTGGTATA TGGACGCTGG 
GAGGACAATA TCATTTGGGA TGCTCAGGCC ATGCCCAGGC TGTTGGAGCC TCCTGTTTTG 
ACACTTGATC CCAATGATGA GAACCTTATT TTGGAAATTC CTGATGAAAA GGAAGAGGCC 
ACTTCTAACT CCCCCTCCAA GGAAAATAAG AAAGAATCAT CTCTGAAGAA GAGCCGAATT 
CTCTTAGGGA AAACAGGGGT CATCAAGGAG GAACCACAGC AGAACATGTC TCAGCCAGAA 
GTGAAAGATC CATGGAATCT CTCCAATGAT GAATATTACT ACCCCAAGCA ACAGGGTCTT 
CGAGGCACCT TTGGAGGAAA TATTATCCAG CACTCAATTC CTGCAGTGGA ATTACGACAG 
CCCTTCTTTC CTACCCATAT GGGGCCCATC AAACTTCGGC AGTTCCATCG TCCACCCCTG 
AAGAAATATT CCTTCGGGGC ACTCTCTCAG CCAGGTCCCC ACTCAGTCCA GCCCTTGCTG 
AAGCACATCA AAAAGAAGGC TAAGATGAGA GAACAAGAGA GACAAGCTTC TGGTGGTGGA 
GAGATGTTTT TTATGCGCAC ACCTCAGGAC CTCACAGGCA AAGATGGAGA TCTTATTCTT 
GCAGAATACA GTGAGGAAAA TGGACCATTA ATGATGCAGG TTGGCATGGC AACCAAGATA 
AAAAACTATT ATAAGCGGAA ACCTGGAAAA GATCCTGGGG CCCCAGATTG TAAATATGGA 
GAAACTGTTT ACTGTCATAC ATCTCCTTTC CTAGGCTCTC TTCATCCTGG TCAATTACTG 
CAGGCATTTG AGAACAATCT CTTTCGTGCC CCAATTTATC TTCATAAGAT GCCAGAAACT 
GACTTCCTTA TTATTCGGAC AAGACAAGGT TACTACATTC GGGAGTTAGT GGATATTTTT 
GTGGTCGGCC AGCAATGCCC CTTGTTTGAA GTTCCTGGGC CCAACTCCAA AAGAGCTAAT 
ACACATATTC GAGACTTTCT CCAGGTTTTT ATTTACCGCC TCTTCTGGAA GAGTAAAGAT 
CGGCCAAGGC GGATCCGAAT GGAAGATATA AAAAAAGCCT TTCCTTCTCA CTCAGAAAGT 
AGCATCCGCA AGAGGCTAAA GCTCTGCGCT GACTTCAAAC GCACAGGGAT GGACTCAAAC 
TGGTGGGTGC TAAAGTCAGA TTTTCGTTTA CCAACTGAAG AGGAGATCAG AGCTATGGTG 
TCTCCAGAGC AGTGCTGTGC TTATTATAGC ATGATAGCTG CAGAGCAGCG ACTAAAGGAT 
GCTGGCTATG GTGAGAAGTC CTTTTTTGCT CCAGAAGAAG AAAATGAGGA AGATTTCCAG 
ATGAAGATTG ATGATGAAGT TCGCACTGCT CCTTGGAATA CCACAAGGGC TTTCATTGCT 
GCCATGAAGG GCAAGTGTCT CTTGGAGGTG ACTGGGGTGG CAGATCCCAC AGGGTGTGGT 
GAAGGATTCT CCTATGTCAA GATTCCAAAC AAACCAACAC AGCAGAAGGA TGATAAGGAG 
CCTCAGCCAG TGAAGAAGAC AGTGACAGGA ACAGATGCAG ATCTCCGTCG CCTCTCACTG 
AAAAATGCCA AGCAGCTCCT ACGTAAATTT GGTGTGCCTG AGGAAGAGAT TAAAAAGCTG 
TCCCGCTGGG AAGTGATTGA TGTGGTACGC ACAATGTCAA CAGAGCAAGC CCGCTCTGGA 
GAGGGGCCCA TGAGTAAATT TGCCCGTGGA TCAAGGTTTT CTGTGGCTGA GCATCAAGAG 
CGTTACAAAG AGGAATGTCA GCGCATCTTT GATCTACAGA ACAAGGTTTT GTCATCAACT 
GAAATATTGT CAACTGACAC AGACAGCAGC TCAGCCGAAG ACAGTGACTT TGAAGAAATG 
GGAAAGAACA TTGAGAACAT GTTGCAGAAT AAGAAAACCA GCTCTCAGCT GTCACGTGAA 
CGAGAGGAGC AGGAGCGGAA GGAACTGCAG CGGATGCTAC TGGCAGCAGG TTCTGCAGCA 
TCAGGAAACA ATCACAGAGA TGATGATACA GCTTCTGTGA CCAGCCTTAA CTCTTCTGCC 
ACTGGCCGTT GTCTCAAAAT TTATCGCACA TTTCGAGATG AAGAAGGGAA AGAGTATGTT 
CGCTGTGAGA CAGTCCGAAA GCCTGCTGTC ATTGATGCCT ATGTGCGCAT ACGGACCACA 
AAAGATGAGG AATTCATTCG AAAGTTTGCC CTTTTTGATG AACAGCATCG AGAAGAGATG 
CGAAAGGAAC GGCGGAGGAT TCAAGAGCAA CTTAGGCGGC TCAAGCGGAA TCAGGAAAAG 
GAGAAGCTTA AGGGTCCCCC TGAGAAGAAG CCTAAAAAAA TGAAGGAGCG TCCTGATCTA 
AAACTGAAAT GTGGAGCTTG TGGTGCCATT GGGCACATGA GGACAAACAA GTTCTGCCCC 
CTCTACTATC AAACAAATGC TCCACCTTCC AACCCTGTTG CCATGACAGA GGAGCAGGAG 
GAGGAGTTGG AAAAGACAGT CATTCATAAT GATAATGAAG AACTTATCAA GGTTGAAGGG 
ACCAAGATCG TCCTGGGAAA ACAGCTAATT GAGAGTGCAG ATGAGGTTCG AAGAAAATCT 
CTGGTTCTCA AGTTCCCTAA ACAGCAACTT CCTCCCAAGA AGAAACGGCG AGTTGGAACC 
ACTGTTCACT GTGACTACTT AAATAGACCT CATAAATCCA TCCACCGGCG CCGGACAGAC 
CCAATGGTGA CATTGTCCTC CATCTTGGAG TCTATCATCA ATGACATGAG AGATCTTCCA 
AATACCTACC CTTTCCACAC TCCAGTCAAT GCAAAGGTTG TAAAGGACTA CTACAAAATC 
ATCACTCGAC CAATGGACTT ACAAACACTC CGTGAAAATG TGCGCAAACG CCTTTACCCA 
TCTCGGGAAG AGTTCAGAGA ACATTTGGAG CTCATTGTGA AAAATAGTGC AACCTATAAT 
GGTCCGAAGC ACTCATTGAC CCAGATCTCT CAATCTATGC TGGATCTCTG TGATGAAAAA 
CTCAAAGAGA AAGAAGATAA ATTGGCTCGT TTAGAGAAAG CTATCAACCC CTTGCTGGAT 
GATGATGACC AAGTAGCATT TTCTTTCATT CTGGACAACA TTGTCACCCA GAAAATGATG 
GCAGTTCCAG ATTCTTGGCC ATTTCATCAC CCAGTTAATA AGAAGTTTGT TCCAGATTAT 
TACAAAGTGA TTATCAGTCC AATGGATTTA GAGACTATAC GTAAGAATAT CTCCAAGCAC 
AAGTACCAGA GTCGGGAGAG CTTTCTGGAT GATGTCAACC TTATTCTGGC CAACAGTGTT 
AAGTATAATG GGCCTGAAAG TCAGTATACT AAGACTGCTC AGGAGATTGT AAATGTCTGT 
TACCAGACAT TGACTGAGTA TGATGAACAT TTGACTCAAC TTGAGAAGGA TATTTGTACA 
GCTAAGGAAG CGGCTTTGGA GGAAGCAGAA TTAGAAAGCT TGGACCCAAT GACCCCAGGG 
CCTTATACAC CTCAGCCTCC TGATTTGTAT GACACCAACA CATCCCTCAG TATGTCCCGA 
GATGCCTCTG TATTTCAAGA TGAGAGCAAT ATGTCTGTCC TGGATATTCC CACTGCCACT 
CCAGAAAAGC AGGTGACACA GGAAGGTGAA GATGCAGATG GTGATCTTGC AGATGAAGAG 
GAAGGAAGTA TGCAACAGCC TCAAGCCAGT GTCCTGTATG AGGATTTGCT TATGTCTGAA 
GGAGAAGATG ATGAAGAAGA TGCTGGGAGT GATGAAGAAG GAGACAATCC TTTCTCTGCT 
ATCCAACTGA GTGAAAGTGG AAGTGACTCT GATGTTGGAT CTAGTGGGAT AAGACCCAAA 
CAGCCCCGCA TGCTTCAGGA AAACACAAGG ATGGGCATGG AAAATGAAGA AAGCATGATG 
TCCTATGAGG GAGATGGTGG GGAGGCTTCT CATGGTTTGG AGGATAGCAA TATGAGTTAT 
GGGAGCTATG AGGAGCCTGA TCCCAAGTCG AACACCCAAG ATACAAGCTT CAGCAGCATC 
GGTGGGTATG AGGTATCAGA GGAAGAAGAA GATGAGGAGG AAGAGCAGCG CTCTGGGCCA 
AGTGTACTAA GCCAGGTCCA CCTGTCAGAG GATGAGGAGG ACAGTGAGGA TTTCCACTCC 
ATTGCTGGGG ACAGTGATTT GGACTCTGAT GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_849327.1
CDS	93..5708
Translation	MGPGWDLLLPVAAGDTTSAIMSDTDSDEDSAGGGPFSLTGFLFGNINGAGQLEGESVLDDECKKHLAGLGALGLGSLITELTANEELAGTDGALVNDEGWIRSTEDAVDYSDITEVAEDESRRYQQTMGSLQPLCHSDYDEDDYDADCEDIDCKLMPPPPPPPGPMKKDKDQDGLTGEKVDFSSSSDSESEMGPQEASQAESEDGKLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSARRKRKKKHRELIQEEQIQEVECSVESEVNKKSLWNYDYAPPPPPEQCLSDDEITMMAPVESKFSQSTGDIDKVTDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKLRKMDHEPVIKCRMMEDLRKFEENNGSDLLADENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDKPWYSIFPIDNEELVYGRWEDNIIWDAQAMPRLLEPPVLTLDPNDENLILEIPDEKEEATSNSPSKENKKESSLKKSRILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYYIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYYSMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEILSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMLLAAGSAASGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKPAVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIISPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNGPESQYTKTAQEIVNVCYQTLTEYDEHLTQLEKDICTAKEAALEEAELESLDPMTPGPYTPQPPDLYDTNTSLSMSRDASVFQDESNMSVLDIPTATPEKQVTQEGEDADGDLADEEEGSMQQPQASVLYEDLLMSEGEDDEEDAGSDEEGDNPFSAIQLSESGSDSDVGSSGIRPKQPRMLQENTRMGMENEESMMSYEGDGGEASHGLEDSNMSYGSYEEPDPKSNTQDTSFSSIGGYEVSEEEEDEEEEQRSGPSVLSQVHLSEDEEDSEDFHSIAGDSDLDSDE

Target ORF information:

RefSeq Version	XM_844234.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa (TAF1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_844234.4

ORF Insert Sequence:

ATGGGACCTG GCTGGGATTT GCTGCTGCCT GTAGCCGCCG GAGACACTAC CAGCGCCATC 
ATGTCAGACA CGGACAGCGA TGAAGATTCT GCTGGAGGCG GCCCATTTTC TCTAACCGGT 
TTCCTTTTTG GCAACATCAA TGGAGCCGGG CAGCTGGAGG GGGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGTC TGGGCAGCCT GATCACTGAA 
CTCACGGCAA ACGAAGAATT GGCTGGGACC GATGGTGCCC TGGTAAATGA TGAAGGATGG 
ATCAGGAGTA CAGAAGATGC TGTGGACTAT TCAGACATCA CTGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ATCAGCAGAC AATGGGGAGC TTGCAGCCCC TTTGCCACTC AGATTATGAT 
GAAGATGACT ATGATGCCGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAATGAA GAAAGATAAG GACCAGGATG GTCTTACCGG TGAGAAAGTG 
GACTTCAGTA GTTCCTCTGA CTCAGAATCT GAGATGGGAC CTCAGGAAGC ATCACAGGCA 
GAATCTGAGG ATGGAAAGCT GACCCTACCA TTGGCTGGGA TTATGCAACA CGATGCCACC 
AAGCTGTTGC CAAGTGTCAC AGAACTTTTC CCGGAATTTC GGCCTGGGAA GGTATTACGC 
TTCCTTCGTC TTTTCGGACC AGGGAAGAAT GTCCCATCTG TTTGGCGGAG TGCTCGGAGA 
AAGAGGAAGA AGAAGCACCG TGAGCTGATA CAGGAAGAAC AGATCCAGGA GGTGGAGTGC 
TCAGTAGAGT CCGAAGTCAA CAAGAAGTCT TTATGGAATT ATGACTATGC TCCACCACCA 
CCTCCAGAGC AGTGTCTCTC TGATGATGAA ATTACAATGA TGGCTCCTGT TGAATCAAAG 
TTTTCCCAGT CAACTGGAGA TATAGATAAA GTGACAGATA CCAAACCAAG AGTGGCTGAG 
TGGCGTTATG GGCCTGCTCG ACTGTGGTAT GATATGCTGG GTGTCCCTGA AGATGGCAGT 
GGGTTTGATT ATGGCTTCAA ACTGAGAAAA ATGGACCATG AGCCTGTGAT AAAATGTAGA 
ATGATGGAGG ACTTGAGGAA ATTTGAGGAA AACAATGGCT CTGACCTACT GGCTGATGAA 
AACTTCCTGA TGGTGACACA GCTGCACTGG GAGGATGATA TCATCTGGGA TGGGGAGGAT 
GTCAAGCACA AAGGAACAAA GCCTCAGCGT GCAAGCCTGG CAGGCTGGCT TCCTTCCAGC 
ATGACTAGGA ATGCCATGGC CTACAATGTT CAGCAAGGTT TTGCAGCCAC CCTGGATGAT 
GACAAACCTT GGTACTCCAT CTTTCCCATT GACAACGAGG AGCTGGTATA TGGACGCTGG 
GAGGACAATA TCATTTGGGA TGCTCAGGCC ATGCCCAGGC TGTTGGAGCC TCCTGTTTTG 
ACACTTGATC CCAATGATGA GAACCTTATT TTGGAAATTC CTGATGAAAA GGAAGAGGCC 
ACTTCTAACT CCCCCTCCAA GGAAAATAAG AAAGAATCAT CTCTGAAGAA GAGCCGAATT 
CTCTTAGGGA AAACAGGGGT CATCAAGGAG GAACCACAGC AGAACATGTC TCAGCCAGAA 
GTGAAAGATC CATGGAATCT CTCCAATGAT GAATATTACT ACCCCAAGCA ACAGGGTCTT 
CGAGGCACCT TTGGAGGAAA TATTATCCAG CACTCAATTC CTGCAGTGGA ATTACGACAG 
CCCTTCTTTC CTACCCATAT GGGGCCCATC AAACTTCGGC AGTTCCATCG TCCACCCCTG 
AAGAAATATT CCTTCGGGGC ACTCTCTCAG CCAGGTCCCC ACTCAGTCCA GCCCTTGCTG 
AAGCACATCA AAAAGAAGGC TAAGATGAGA GAACAAGAGA GACAAGCTTC TGGTGGTGGA 
GAGATGTTTT TTATGCGCAC ACCTCAGGAC CTCACAGGCA AAGATGGAGA TCTTATTCTT 
GCAGAATACA GTGAGGAAAA TGGACCATTA ATGATGCAGG TTGGCATGGC AACCAAGATA 
AAAAACTATT ATAAGCGGAA ACCTGGAAAA GATCCTGGGG CCCCAGATTG TAAATATGGA 
GAAACTGTTT ACTGTCATAC ATCTCCTTTC CTAGGCTCTC TTCATCCTGG TCAATTACTG 
CAGGCATTTG AGAACAATCT CTTTCGTGCC CCAATTTATC TTCATAAGAT GCCAGAAACT 
GACTTCCTTA TTATTCGGAC AAGACAAGGT TACTACATTC GGGAGTTAGT GGATATTTTT 
GTGGTCGGCC AGCAATGCCC CTTGTTTGAA GTTCCTGGGC CCAACTCCAA AAGAGCTAAT 
ACACATATTC GAGACTTTCT CCAGGTTTTT ATTTACCGCC TCTTCTGGAA GAGTAAAGAT 
CGGCCAAGGC GGATCCGAAT GGAAGATATA AAAAAAGCCT TTCCTTCTCA CTCAGAAAGT 
AGCATCCGCA AGAGGCTAAA GCTCTGCGCT GACTTCAAAC GCACAGGGAT GGACTCAAAC 
TGGTGGGTGC TAAAGTCAGA TTTTCGTTTA CCAACTGAAG AGGAGATCAG AGCTATGGTG 
TCTCCAGAGC AGTGCTGTGC TTATTATAGC ATGATAGCTG CAGAGCAGCG ACTAAAGGAT 
GCTGGCTATG GTGAGAAGTC CTTTTTTGCT CCAGAAGAAG AAAATGAGGA AGATTTCCAG 
ATGAAGATTG ATGATGAAGT TCGCACTGCT CCTTGGAATA CCACAAGGGC TTTCATTGCT 
GCCATGAAGG GCAAGTGTCT CTTGGAGGTG ACTGGGGTGG CAGATCCCAC AGGGTGTGGT 
GAAGGATTCT CCTATGTCAA GATTCCAAAC AAACCAACAC AGCAGAAGGA TGATAAGGAG 
CCTCAGCCAG TGAAGAAGAC AGTGACAGGA ACAGATGCAG ATCTCCGTCG CCTCTCACTG 
AAAAATGCCA AGCAGCTCCT ACGTAAATTT GGTGTGCCTG AGGAAGAGAT TAAAAAGCTG 
TCCCGCTGGG AAGTGATTGA TGTGGTACGC ACAATGTCAA CAGAGCAAGC CCGCTCTGGA 
GAGGGGCCCA TGAGTAAATT TGCCCGTGGA TCAAGGTTTT CTGTGGCTGA GCATCAAGAG 
CGTTACAAAG AGGAATGTCA GCGCATCTTT GATCTACAGA ACAAGGTTTT GTCATCAACT 
GAAATATTGT CAACTGACAC AGACAGCAGC TCAGCCGAAG ACAGTGACTT TGAAGAAATG 
GGAAAGAACA TTGAGAACAT GTTGCAGAAT AAGAAAACCA GCTCTCAGCT GTCACGTGAA 
CGAGAGGAGC AGGAGCGGAA GGAACTGCAG CGGATGCTAC TGGCAGCAGG TTCTGCAGCA 
TCAGGAAACA ATCACAGAGA TGATGATACA GCTTCTGTGA CCAGCCTTAA CTCTTCTGCC 
ACTGGCCGTT GTCTCAAAAT TTATCGCACA TTTCGAGATG AAGAAGGGAA AGAGTATGTT 
CGCTGTGAGA CAGTCCGAAA GCCTGCTGTC ATTGATGCCT ATGTGCGCAT ACGGACCACA 
AAAGATGAGG AATTCATTCG AAAGTTTGCC CTTTTTGATG AACAGCATCG AGAAGAGATG 
CGAAAGGAAC GGCGGAGGAT TCAAGAGCAA CTTAGGCGGC TCAAGCGGAA TCAGGAAAAG 
GAGAAGCTTA AGGGTCCCCC TGAGAAGAAG CCTAAAAAAA TGAAGGAGCG TCCTGATCTA 
AAACTGAAAT GTGGAGCTTG TGGTGCCATT GGGCACATGA GGACAAACAA GTTCTGCCCC 
CTCTACTATC AAACAAATGC TCCACCTTCC AACCCTGTTG CCATGACAGA GGAGCAGGAG 
GAGGAGTTGG AAAAGACAGT CATTCATAAT GATAATGAAG AACTTATCAA GGTTGAAGGG 
ACCAAGATCG TCCTGGGAAA ACAGCTAATT GAGAGTGCAG ATGAGGTTCG AAGAAAATCT 
CTGGTTCTCA AGTTCCCTAA ACAGCAACTT CCTCCCAAGA AGAAACGGCG AGTTGGAACC 
ACTGTTCACT GTGACTACTT AAATAGACCT CATAAATCCA TCCACCGGCG CCGGACAGAC 
CCAATGGTGA CATTGTCCTC CATCTTGGAG TCTATCATCA ATGACATGAG AGATCTTCCA 
AATACCTACC CTTTCCACAC TCCAGTCAAT GCAAAGGTTG TAAAGGACTA CTACAAAATC 
ATCACTCGAC CAATGGACTT ACAAACACTC CGTGAAAATG TGCGCAAACG CCTTTACCCA 
TCTCGGGAAG AGTTCAGAGA ACATTTGGAG CTCATTGTGA AAAATAGTGC AACCTATAAT 
GGTCCGAAGC ACTCATTGAC CCAGATCTCT CAATCTATGC TGGATCTCTG TGATGAAAAA 
CTCAAAGAGA AAGAAGATAA ATTGGCTCGT TTAGAGAAAG CTATCAACCC CTTGCTGGAT 
GATGATGACC AAGTAGCATT TTCTTTCATT CTGGACAACA TTGTCACCCA GAAAATGATG 
GCAGTTCCAG ATTCTTGGCC ATTTCATCAC CCAGTTAATA AGAAGTTTGT TCCAGATTAT 
TACAAAGTGA TTATCAGTCC AATGGATTTA GAGACTATAC GTAAGAATAT CTCCAAGCAC 
AAGTACCAGA GTCGGGAGAG CTTTCTGGAT GATGTCAACC TTATTCTGGC CAACAGTGTT 
AAGTATAATG GGCCTGAAAG TCAGTATACT AAGACTGCTC AGGAGATTGT AAATGTCTGT 
TACCAGACAT TGACTGAGTA TGATGAACAT TTGACTCAAC TTGAGAAGGA TATTTGTACA 
GCTAAGGAAG CGGCTTTGGA GGAAGCAGAA TTAGAAAGCT TGGACCCAAT GACCCCAGGG 
CCTTATACAC CTCAGCCTCC TGATTTGTAT GACACCAACA CATCCCTCAG TATGTCCCGA 
GATGCCTCTG TATTTCAAGA TGAGAGCAAT ATGTCTGTCC TGGATATTCC CACTGCCACT 
CCAGAAAAGC AGGTGACACA GGAAGGTGAA GATGCAGATG GTGATCTTGC AGATGAAGAG 
GAAGGAAGTA TGCAACAGCC TCAAGCCAGT GTCCTGTATG AGGATTTGCT TATGTCTGAA 
GGAGAAGATG ATGAAGAAGA TGCTGGGAGT GATGAAGAAG GAGACAATCC TTTCTCTGCT 
ATCCAACTGA GTGAAAGTGG AAGTGACTCT GATGTTGGAT CTAGTGGGAT AAGACCCAAA 
CAGCCCCGCA TGCTTCAGGA AAACACAAGG ATGGGCATGG AAAATGAAGA AAGCATGATG 
TCCTATGAGG GAGATGGTGG GGAGGCTTCT CATGGTTTGG AGGATAGCAA TATGAGTTAT 
GGGAGCTATG AGGAGCCTGA TCCCAAGTCG AACACCCAAG ATACAAGCTT CAGCAGCATC 
GGTGGGTATG AGGTATCAGA GGAAGAAGAA GATGAGGAGG AAGAGCAGCG CTCTGGGCCA 
AGTGTACTAA GCCAGGTCCA CCTGTCAGAG GATGAGGAGG ACAGTGAGGA TTTCCACTCC 
ATTGCTGGGG ACAGTGATTT GGACTCTGAT GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAF1 ( XM_844234.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_849327.1 Canis lupus familiaris TATA-box binding protein associated factor 1 (TAF1), transcript variant X8, mRNA.

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CloneID	OCe32533
Clone ID Related Accession (Same CDS sequence)	XM_844234.4 , XM_844234.5
Accession Version	XM_844234.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5616bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	transcription initiation factor TFIID subunit 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_844234.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACCTG GCTGGGATTT GCTGCTGCCT GTAGCCGCCG GAGACACTAC CAGCGCCATC 
ATGTCAGACA CGGACAGCGA TGAAGATTCT GCTGGAGGCG GCCCATTTTC TCTAACCGGT 
TTCCTTTTTG GCAACATCAA TGGAGCCGGG CAGCTGGAGG GGGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGTC TGGGCAGCCT GATCACTGAA 
CTCACGGCAA ACGAAGAATT GGCTGGGACC GATGGTGCCC TGGTAAATGA TGAAGGATGG 
ATCAGGAGTA CAGAAGATGC TGTGGACTAT TCAGACATCA CTGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ATCAGCAGAC AATGGGGAGC TTGCAGCCCC TTTGCCACTC AGATTATGAT 
GAAGATGACT ATGATGCCGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAATGAA GAAAGATAAG GACCAGGATG GTCTTACCGG TGAGAAAGTG 
GACTTCAGTA GTTCCTCTGA CTCAGAATCT GAGATGGGAC CTCAGGAAGC ATCACAGGCA 
GAATCTGAGG ATGGAAAGCT GACCCTACCA TTGGCTGGGA TTATGCAACA CGATGCCACC 
AAGCTGTTGC CAAGTGTCAC AGAACTTTTC CCGGAATTTC GGCCTGGGAA GGTATTACGC 
TTCCTTCGTC TTTTCGGACC AGGGAAGAAT GTCCCATCTG TTTGGCGGAG TGCTCGGAGA 
AAGAGGAAGA AGAAGCACCG TGAGCTGATA CAGGAAGAAC AGATCCAGGA GGTGGAGTGC 
TCAGTAGAGT CCGAAGTCAA CAAGAAGTCT TTATGGAATT ATGACTATGC TCCACCACCA 
CCTCCAGAGC AGTGTCTCTC TGATGATGAA ATTACAATGA TGGCTCCTGT TGAATCAAAG 
TTTTCCCAGT CAACTGGAGA TATAGATAAA GTGACAGATA CCAAACCAAG AGTGGCTGAG 
TGGCGTTATG GGCCTGCTCG ACTGTGGTAT GATATGCTGG GTGTCCCTGA AGATGGCAGT 
GGGTTTGATT ATGGCTTCAA ACTGAGAAAA ATGGACCATG AGCCTGTGAT AAAATGTAGA 
ATGATGGAGG ACTTGAGGAA ATTTGAGGAA AACAATGGCT CTGACCTACT GGCTGATGAA 
AACTTCCTGA TGGTGACACA GCTGCACTGG GAGGATGATA TCATCTGGGA TGGGGAGGAT 
GTCAAGCACA AAGGAACAAA GCCTCAGCGT GCAAGCCTGG CAGGCTGGCT TCCTTCCAGC 
ATGACTAGGA ATGCCATGGC CTACAATGTT CAGCAAGGTT TTGCAGCCAC CCTGGATGAT 
GACAAACCTT GGTACTCCAT CTTTCCCATT GACAACGAGG AGCTGGTATA TGGACGCTGG 
GAGGACAATA TCATTTGGGA TGCTCAGGCC ATGCCCAGGC TGTTGGAGCC TCCTGTTTTG 
ACACTTGATC CCAATGATGA GAACCTTATT TTGGAAATTC CTGATGAAAA GGAAGAGGCC 
ACTTCTAACT CCCCCTCCAA GGAAAATAAG AAAGAATCAT CTCTGAAGAA GAGCCGAATT 
CTCTTAGGGA AAACAGGGGT CATCAAGGAG GAACCACAGC AGAACATGTC TCAGCCAGAA 
GTGAAAGATC CATGGAATCT CTCCAATGAT GAATATTACT ACCCCAAGCA ACAGGGTCTT 
CGAGGCACCT TTGGAGGAAA TATTATCCAG CACTCAATTC CTGCAGTGGA ATTACGACAG 
CCCTTCTTTC CTACCCATAT GGGGCCCATC AAACTTCGGC AGTTCCATCG TCCACCCCTG 
AAGAAATATT CCTTCGGGGC ACTCTCTCAG CCAGGTCCCC ACTCAGTCCA GCCCTTGCTG 
AAGCACATCA AAAAGAAGGC TAAGATGAGA GAACAAGAGA GACAAGCTTC TGGTGGTGGA 
GAGATGTTTT TTATGCGCAC ACCTCAGGAC CTCACAGGCA AAGATGGAGA TCTTATTCTT 
GCAGAATACA GTGAGGAAAA TGGACCATTA ATGATGCAGG TTGGCATGGC AACCAAGATA 
AAAAACTATT ATAAGCGGAA ACCTGGAAAA GATCCTGGGG CCCCAGATTG TAAATATGGA 
GAAACTGTTT ACTGTCATAC ATCTCCTTTC CTAGGCTCTC TTCATCCTGG TCAATTACTG 
CAGGCATTTG AGAACAATCT CTTTCGTGCC CCAATTTATC TTCATAAGAT GCCAGAAACT 
GACTTCCTTA TTATTCGGAC AAGACAAGGT TACTACATTC GGGAGTTAGT GGATATTTTT 
GTGGTCGGCC AGCAATGCCC CTTGTTTGAA GTTCCTGGGC CCAACTCCAA AAGAGCTAAT 
ACACATATTC GAGACTTTCT CCAGGTTTTT ATTTACCGCC TCTTCTGGAA GAGTAAAGAT 
CGGCCAAGGC GGATCCGAAT GGAAGATATA AAAAAAGCCT TTCCTTCTCA CTCAGAAAGT 
AGCATCCGCA AGAGGCTAAA GCTCTGCGCT GACTTCAAAC GCACAGGGAT GGACTCAAAC 
TGGTGGGTGC TAAAGTCAGA TTTTCGTTTA CCAACTGAAG AGGAGATCAG AGCTATGGTG 
TCTCCAGAGC AGTGCTGTGC TTATTATAGC ATGATAGCTG CAGAGCAGCG ACTAAAGGAT 
GCTGGCTATG GTGAGAAGTC CTTTTTTGCT CCAGAAGAAG AAAATGAGGA AGATTTCCAG 
ATGAAGATTG ATGATGAAGT TCGCACTGCT CCTTGGAATA CCACAAGGGC TTTCATTGCT 
GCCATGAAGG GCAAGTGTCT CTTGGAGGTG ACTGGGGTGG CAGATCCCAC AGGGTGTGGT 
GAAGGATTCT CCTATGTCAA GATTCCAAAC AAACCAACAC AGCAGAAGGA TGATAAGGAG 
CCTCAGCCAG TGAAGAAGAC AGTGACAGGA ACAGATGCAG ATCTCCGTCG CCTCTCACTG 
AAAAATGCCA AGCAGCTCCT ACGTAAATTT GGTGTGCCTG AGGAAGAGAT TAAAAAGCTG 
TCCCGCTGGG AAGTGATTGA TGTGGTACGC ACAATGTCAA CAGAGCAAGC CCGCTCTGGA 
GAGGGGCCCA TGAGTAAATT TGCCCGTGGA TCAAGGTTTT CTGTGGCTGA GCATCAAGAG 
CGTTACAAAG AGGAATGTCA GCGCATCTTT GATCTACAGA ACAAGGTTTT GTCATCAACT 
GAAATATTGT CAACTGACAC AGACAGCAGC TCAGCCGAAG ACAGTGACTT TGAAGAAATG 
GGAAAGAACA TTGAGAACAT GTTGCAGAAT AAGAAAACCA GCTCTCAGCT GTCACGTGAA 
CGAGAGGAGC AGGAGCGGAA GGAACTGCAG CGGATGCTAC TGGCAGCAGG TTCTGCAGCA 
TCAGGAAACA ATCACAGAGA TGATGATACA GCTTCTGTGA CCAGCCTTAA CTCTTCTGCC 
ACTGGCCGTT GTCTCAAAAT TTATCGCACA TTTCGAGATG AAGAAGGGAA AGAGTATGTT 
CGCTGTGAGA CAGTCCGAAA GCCTGCTGTC ATTGATGCCT ATGTGCGCAT ACGGACCACA 
AAAGATGAGG AATTCATTCG AAAGTTTGCC CTTTTTGATG AACAGCATCG AGAAGAGATG 
CGAAAGGAAC GGCGGAGGAT TCAAGAGCAA CTTAGGCGGC TCAAGCGGAA TCAGGAAAAG 
GAGAAGCTTA AGGGTCCCCC TGAGAAGAAG CCTAAAAAAA TGAAGGAGCG TCCTGATCTA 
AAACTGAAAT GTGGAGCTTG TGGTGCCATT GGGCACATGA GGACAAACAA GTTCTGCCCC 
CTCTACTATC AAACAAATGC TCCACCTTCC AACCCTGTTG CCATGACAGA GGAGCAGGAG 
GAGGAGTTGG AAAAGACAGT CATTCATAAT GATAATGAAG AACTTATCAA GGTTGAAGGG 
ACCAAGATCG TCCTGGGAAA ACAGCTAATT GAGAGTGCAG ATGAGGTTCG AAGAAAATCT 
CTGGTTCTCA AGTTCCCTAA ACAGCAACTT CCTCCCAAGA AGAAACGGCG AGTTGGAACC 
ACTGTTCACT GTGACTACTT AAATAGACCT CATAAATCCA TCCACCGGCG CCGGACAGAC 
CCAATGGTGA CATTGTCCTC CATCTTGGAG TCTATCATCA ATGACATGAG AGATCTTCCA 
AATACCTACC CTTTCCACAC TCCAGTCAAT GCAAAGGTTG TAAAGGACTA CTACAAAATC 
ATCACTCGAC CAATGGACTT ACAAACACTC CGTGAAAATG TGCGCAAACG CCTTTACCCA 
TCTCGGGAAG AGTTCAGAGA ACATTTGGAG CTCATTGTGA AAAATAGTGC AACCTATAAT 
GGTCCGAAGC ACTCATTGAC CCAGATCTCT CAATCTATGC TGGATCTCTG TGATGAAAAA 
CTCAAAGAGA AAGAAGATAA ATTGGCTCGT TTAGAGAAAG CTATCAACCC CTTGCTGGAT 
GATGATGACC AAGTAGCATT TTCTTTCATT CTGGACAACA TTGTCACCCA GAAAATGATG 
GCAGTTCCAG ATTCTTGGCC ATTTCATCAC CCAGTTAATA AGAAGTTTGT TCCAGATTAT 
TACAAAGTGA TTATCAGTCC AATGGATTTA GAGACTATAC GTAAGAATAT CTCCAAGCAC 
AAGTACCAGA GTCGGGAGAG CTTTCTGGAT GATGTCAACC TTATTCTGGC CAACAGTGTT 
AAGTATAATG GGCCTGAAAG TCAGTATACT AAGACTGCTC AGGAGATTGT AAATGTCTGT 
TACCAGACAT TGACTGAGTA TGATGAACAT TTGACTCAAC TTGAGAAGGA TATTTGTACA 
GCTAAGGAAG CGGCTTTGGA GGAAGCAGAA TTAGAAAGCT TGGACCCAAT GACCCCAGGG 
CCTTATACAC CTCAGCCTCC TGATTTGTAT GACACCAACA CATCCCTCAG TATGTCCCGA 
GATGCCTCTG TATTTCAAGA TGAGAGCAAT ATGTCTGTCC TGGATATTCC CACTGCCACT 
CCAGAAAAGC AGGTGACACA GGAAGGTGAA GATGCAGATG GTGATCTTGC AGATGAAGAG 
GAAGGAAGTA TGCAACAGCC TCAAGCCAGT GTCCTGTATG AGGATTTGCT TATGTCTGAA 
GGAGAAGATG ATGAAGAAGA TGCTGGGAGT GATGAAGAAG GAGACAATCC TTTCTCTGCT 
ATCCAACTGA GTGAAAGTGG AAGTGACTCT GATGTTGGAT CTAGTGGGAT AAGACCCAAA 
CAGCCCCGCA TGCTTCAGGA AAACACAAGG ATGGGCATGG AAAATGAAGA AAGCATGATG 
TCCTATGAGG GAGATGGTGG GGAGGCTTCT CATGGTTTGG AGGATAGCAA TATGAGTTAT 
GGGAGCTATG AGGAGCCTGA TCCCAAGTCG AACACCCAAG ATACAAGCTT CAGCAGCATC 
GGTGGGTATG AGGTATCAGA GGAAGAAGAA GATGAGGAGG AAGAGCAGCG CTCTGGGCCA 
AGTGTACTAA GCCAGGTCCA CCTGTCAGAG GATGAGGAGG ACAGTGAGGA TTTCCACTCC 
ATTGCTGGGG ACAGTGATTT GGACTCTGAT GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_849327.1
CDS	1..5616
Translation	MGPGWDLLLPVAAGDTTSAIMSDTDSDEDSAGGGPFSLTGFLFGNINGAGQLEGESVLDDECKKHLAGLGALGLGSLITELTANEELAGTDGALVNDEGWIRSTEDAVDYSDITEVAEDESRRYQQTMGSLQPLCHSDYDEDDYDADCEDIDCKLMPPPPPPPGPMKKDKDQDGLTGEKVDFSSSSDSESEMGPQEASQAESEDGKLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSARRKRKKKHRELIQEEQIQEVECSVESEVNKKSLWNYDYAPPPPPEQCLSDDEITMMAPVESKFSQSTGDIDKVTDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKLRKMDHEPVIKCRMMEDLRKFEENNGSDLLADENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDKPWYSIFPIDNEELVYGRWEDNIIWDAQAMPRLLEPPVLTLDPNDENLILEIPDEKEEATSNSPSKENKKESSLKKSRILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYYIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYYSMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEILSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMLLAAGSAASGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKPAVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIISPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNGPESQYTKTAQEIVNVCYQTLTEYDEHLTQLEKDICTAKEAALEEAELESLDPMTPGPYTPQPPDLYDTNTSLSMSRDASVFQDESNMSVLDIPTATPEKQVTQEGEDADGDLADEEEGSMQQPQASVLYEDLLMSEGEDDEEDAGSDEEGDNPFSAIQLSESGSDSDVGSSGIRPKQPRMLQENTRMGMENEESMMSYEGDGGEASHGLEDSNMSYGSYEEPDPKSNTQDTSFSSIGGYEVSEEEEDEEEEQRSGPSVLSQVHLSEDEEDSEDFHSIAGDSDLDSDE

Target ORF information:

RefSeq Version	XM_844234.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris TATA-box binding protein associated factor 1 (TAF1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_844234.5

ORF Insert Sequence:

ATGGGACCTG GCTGGGATTT GCTGCTGCCT GTAGCCGCCG GAGACACTAC CAGCGCCATC 
ATGTCAGACA CGGACAGCGA TGAAGATTCT GCTGGAGGCG GCCCATTTTC TCTAACCGGT 
TTCCTTTTTG GCAACATCAA TGGAGCCGGG CAGCTGGAGG GGGAAAGCGT CTTGGACGAT 
GAGTGTAAGA AGCACTTGGC AGGCTTGGGG GCTTTGGGTC TGGGCAGCCT GATCACTGAA 
CTCACGGCAA ACGAAGAATT GGCTGGGACC GATGGTGCCC TGGTAAATGA TGAAGGATGG 
ATCAGGAGTA CAGAAGATGC TGTGGACTAT TCAGACATCA CTGAGGTAGC AGAAGATGAA 
AGCCGAAGAT ATCAGCAGAC AATGGGGAGC TTGCAGCCCC TTTGCCACTC AGATTATGAT 
GAAGATGACT ATGATGCCGA TTGTGAAGAC ATTGATTGCA AGTTGATGCC TCCTCCACCT 
CCACCTCCAG GACCAATGAA GAAAGATAAG GACCAGGATG GTCTTACCGG TGAGAAAGTG 
GACTTCAGTA GTTCCTCTGA CTCAGAATCT GAGATGGGAC CTCAGGAAGC ATCACAGGCA 
GAATCTGAGG ATGGAAAGCT GACCCTACCA TTGGCTGGGA TTATGCAACA CGATGCCACC 
AAGCTGTTGC CAAGTGTCAC AGAACTTTTC CCGGAATTTC GGCCTGGGAA GGTATTACGC 
TTCCTTCGTC TTTTCGGACC AGGGAAGAAT GTCCCATCTG TTTGGCGGAG TGCTCGGAGA 
AAGAGGAAGA AGAAGCACCG TGAGCTGATA CAGGAAGAAC AGATCCAGGA GGTGGAGTGC 
TCAGTAGAGT CCGAAGTCAA CAAGAAGTCT TTATGGAATT ATGACTATGC TCCACCACCA 
CCTCCAGAGC AGTGTCTCTC TGATGATGAA ATTACAATGA TGGCTCCTGT TGAATCAAAG 
TTTTCCCAGT CAACTGGAGA TATAGATAAA GTGACAGATA CCAAACCAAG AGTGGCTGAG 
TGGCGTTATG GGCCTGCTCG ACTGTGGTAT GATATGCTGG GTGTCCCTGA AGATGGCAGT 
GGGTTTGATT ATGGCTTCAA ACTGAGAAAA ATGGACCATG AGCCTGTGAT AAAATGTAGA 
ATGATGGAGG ACTTGAGGAA ATTTGAGGAA AACAATGGCT CTGACCTACT GGCTGATGAA 
AACTTCCTGA TGGTGACACA GCTGCACTGG GAGGATGATA TCATCTGGGA TGGGGAGGAT 
GTCAAGCACA AAGGAACAAA GCCTCAGCGT GCAAGCCTGG CAGGCTGGCT TCCTTCCAGC 
ATGACTAGGA ATGCCATGGC CTACAATGTT CAGCAAGGTT TTGCAGCCAC CCTGGATGAT 
GACAAACCTT GGTACTCCAT CTTTCCCATT GACAACGAGG AGCTGGTATA TGGACGCTGG 
GAGGACAATA TCATTTGGGA TGCTCAGGCC ATGCCCAGGC TGTTGGAGCC TCCTGTTTTG 
ACACTTGATC CCAATGATGA GAACCTTATT TTGGAAATTC CTGATGAAAA GGAAGAGGCC 
ACTTCTAACT CCCCCTCCAA GGAAAATAAG AAAGAATCAT CTCTGAAGAA GAGCCGAATT 
CTCTTAGGGA AAACAGGGGT CATCAAGGAG GAACCACAGC AGAACATGTC TCAGCCAGAA 
GTGAAAGATC CATGGAATCT CTCCAATGAT GAATATTACT ACCCCAAGCA ACAGGGTCTT 
CGAGGCACCT TTGGAGGAAA TATTATCCAG CACTCAATTC CTGCAGTGGA ATTACGACAG 
CCCTTCTTTC CTACCCATAT GGGGCCCATC AAACTTCGGC AGTTCCATCG TCCACCCCTG 
AAGAAATATT CCTTCGGGGC ACTCTCTCAG CCAGGTCCCC ACTCAGTCCA GCCCTTGCTG 
AAGCACATCA AAAAGAAGGC TAAGATGAGA GAACAAGAGA GACAAGCTTC TGGTGGTGGA 
GAGATGTTTT TTATGCGCAC ACCTCAGGAC CTCACAGGCA AAGATGGAGA TCTTATTCTT 
GCAGAATACA GTGAGGAAAA TGGACCATTA ATGATGCAGG TTGGCATGGC AACCAAGATA 
AAAAACTATT ATAAGCGGAA ACCTGGAAAA GATCCTGGGG CCCCAGATTG TAAATATGGA 
GAAACTGTTT ACTGTCATAC ATCTCCTTTC CTAGGCTCTC TTCATCCTGG TCAATTACTG 
CAGGCATTTG AGAACAATCT CTTTCGTGCC CCAATTTATC TTCATAAGAT GCCAGAAACT 
GACTTCCTTA TTATTCGGAC AAGACAAGGT TACTACATTC GGGAGTTAGT GGATATTTTT 
GTGGTCGGCC AGCAATGCCC CTTGTTTGAA GTTCCTGGGC CCAACTCCAA AAGAGCTAAT 
ACACATATTC GAGACTTTCT CCAGGTTTTT ATTTACCGCC TCTTCTGGAA GAGTAAAGAT 
CGGCCAAGGC GGATCCGAAT GGAAGATATA AAAAAAGCCT TTCCTTCTCA CTCAGAAAGT 
AGCATCCGCA AGAGGCTAAA GCTCTGCGCT GACTTCAAAC GCACAGGGAT GGACTCAAAC 
TGGTGGGTGC TAAAGTCAGA TTTTCGTTTA CCAACTGAAG AGGAGATCAG AGCTATGGTG 
TCTCCAGAGC AGTGCTGTGC TTATTATAGC ATGATAGCTG CAGAGCAGCG ACTAAAGGAT 
GCTGGCTATG GTGAGAAGTC CTTTTTTGCT CCAGAAGAAG AAAATGAGGA AGATTTCCAG 
ATGAAGATTG ATGATGAAGT TCGCACTGCT CCTTGGAATA CCACAAGGGC TTTCATTGCT 
GCCATGAAGG GCAAGTGTCT CTTGGAGGTG ACTGGGGTGG CAGATCCCAC AGGGTGTGGT 
GAAGGATTCT CCTATGTCAA GATTCCAAAC AAACCAACAC AGCAGAAGGA TGATAAGGAG 
CCTCAGCCAG TGAAGAAGAC AGTGACAGGA ACAGATGCAG ATCTCCGTCG CCTCTCACTG 
AAAAATGCCA AGCAGCTCCT ACGTAAATTT GGTGTGCCTG AGGAAGAGAT TAAAAAGCTG 
TCCCGCTGGG AAGTGATTGA TGTGGTACGC ACAATGTCAA CAGAGCAAGC CCGCTCTGGA 
GAGGGGCCCA TGAGTAAATT TGCCCGTGGA TCAAGGTTTT CTGTGGCTGA GCATCAAGAG 
CGTTACAAAG AGGAATGTCA GCGCATCTTT GATCTACAGA ACAAGGTTTT GTCATCAACT 
GAAATATTGT CAACTGACAC AGACAGCAGC TCAGCCGAAG ACAGTGACTT TGAAGAAATG 
GGAAAGAACA TTGAGAACAT GTTGCAGAAT AAGAAAACCA GCTCTCAGCT GTCACGTGAA 
CGAGAGGAGC AGGAGCGGAA GGAACTGCAG CGGATGCTAC TGGCAGCAGG TTCTGCAGCA 
TCAGGAAACA ATCACAGAGA TGATGATACA GCTTCTGTGA CCAGCCTTAA CTCTTCTGCC 
ACTGGCCGTT GTCTCAAAAT TTATCGCACA TTTCGAGATG AAGAAGGGAA AGAGTATGTT 
CGCTGTGAGA CAGTCCGAAA GCCTGCTGTC ATTGATGCCT ATGTGCGCAT ACGGACCACA 
AAAGATGAGG AATTCATTCG AAAGTTTGCC CTTTTTGATG AACAGCATCG AGAAGAGATG 
CGAAAGGAAC GGCGGAGGAT TCAAGAGCAA CTTAGGCGGC TCAAGCGGAA TCAGGAAAAG 
GAGAAGCTTA AGGGTCCCCC TGAGAAGAAG CCTAAAAAAA TGAAGGAGCG TCCTGATCTA 
AAACTGAAAT GTGGAGCTTG TGGTGCCATT GGGCACATGA GGACAAACAA GTTCTGCCCC 
CTCTACTATC AAACAAATGC TCCACCTTCC AACCCTGTTG CCATGACAGA GGAGCAGGAG 
GAGGAGTTGG AAAAGACAGT CATTCATAAT GATAATGAAG AACTTATCAA GGTTGAAGGG 
ACCAAGATCG TCCTGGGAAA ACAGCTAATT GAGAGTGCAG ATGAGGTTCG AAGAAAATCT 
CTGGTTCTCA AGTTCCCTAA ACAGCAACTT CCTCCCAAGA AGAAACGGCG AGTTGGAACC 
ACTGTTCACT GTGACTACTT AAATAGACCT CATAAATCCA TCCACCGGCG CCGGACAGAC 
CCAATGGTGA CATTGTCCTC CATCTTGGAG TCTATCATCA ATGACATGAG AGATCTTCCA 
AATACCTACC CTTTCCACAC TCCAGTCAAT GCAAAGGTTG TAAAGGACTA CTACAAAATC 
ATCACTCGAC CAATGGACTT ACAAACACTC CGTGAAAATG TGCGCAAACG CCTTTACCCA 
TCTCGGGAAG AGTTCAGAGA ACATTTGGAG CTCATTGTGA AAAATAGTGC AACCTATAAT 
GGTCCGAAGC ACTCATTGAC CCAGATCTCT CAATCTATGC TGGATCTCTG TGATGAAAAA 
CTCAAAGAGA AAGAAGATAA ATTGGCTCGT TTAGAGAAAG CTATCAACCC CTTGCTGGAT 
GATGATGACC AAGTAGCATT TTCTTTCATT CTGGACAACA TTGTCACCCA GAAAATGATG 
GCAGTTCCAG ATTCTTGGCC ATTTCATCAC CCAGTTAATA AGAAGTTTGT TCCAGATTAT 
TACAAAGTGA TTATCAGTCC AATGGATTTA GAGACTATAC GTAAGAATAT CTCCAAGCAC 
AAGTACCAGA GTCGGGAGAG CTTTCTGGAT GATGTCAACC TTATTCTGGC CAACAGTGTT 
AAGTATAATG GGCCTGAAAG TCAGTATACT AAGACTGCTC AGGAGATTGT AAATGTCTGT 
TACCAGACAT TGACTGAGTA TGATGAACAT TTGACTCAAC TTGAGAAGGA TATTTGTACA 
GCTAAGGAAG CGGCTTTGGA GGAAGCAGAA TTAGAAAGCT TGGACCCAAT GACCCCAGGG 
CCTTATACAC CTCAGCCTCC TGATTTGTAT GACACCAACA CATCCCTCAG TATGTCCCGA 
GATGCCTCTG TATTTCAAGA TGAGAGCAAT ATGTCTGTCC TGGATATTCC CACTGCCACT 
CCAGAAAAGC AGGTGACACA GGAAGGTGAA GATGCAGATG GTGATCTTGC AGATGAAGAG 
GAAGGAAGTA TGCAACAGCC TCAAGCCAGT GTCCTGTATG AGGATTTGCT TATGTCTGAA 
GGAGAAGATG ATGAAGAAGA TGCTGGGAGT GATGAAGAAG GAGACAATCC TTTCTCTGCT 
ATCCAACTGA GTGAAAGTGG AAGTGACTCT GATGTTGGAT CTAGTGGGAT AAGACCCAAA 
CAGCCCCGCA TGCTTCAGGA AAACACAAGG ATGGGCATGG AAAATGAAGA AAGCATGATG 
TCCTATGAGG GAGATGGTGG GGAGGCTTCT CATGGTTTGG AGGATAGCAA TATGAGTTAT 
GGGAGCTATG AGGAGCCTGA TCCCAAGTCG AACACCCAAG ATACAAGCTT CAGCAGCATC 
GGTGGGTATG AGGTATCAGA GGAAGAAGAA GATGAGGAGG AAGAGCAGCG CTCTGGGCCA 
AGTGTACTAA GCCAGGTCCA CCTGTCAGAG GATGAGGAGG ACAGTGAGGA TTTCCACTCC 
ATTGCTGGGG ACAGTGATTT GGACTCTGAT GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	TAF1
Entrez Gene ID	491950
Full Name	TATA-box binding protein associated factor 1
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003882	XP_308108.5
Xenopus tropicalis (tropical clawed frog)	taf1	XP_002934954.2
Canis lupus familiaris (dog)	TAF1	XP_005641531.1
Mus musculus (house mouse)	Taf1	NP_001074477.1
Danio rerio (zebrafish)	taf1	NP_001038250.1
Drosophila melanogaster (fruit fly)	Taf1	NP_996159.1
Caenorhabditis elegans (roundworm)	taf-1	NP_493426.2
Homo sapiens (human)	TAF1	NP_004597.2
Rattus norvegicus (Norway rat)	Taf1	NP_001178652.1
Bos taurus (cattle)	TAF1	XP_005228102.1
Gallus gallus (chicken)	TAF1	XP_420198.4
	TAF1	XP_002808585.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

TAF1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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